LOCUS       DAOOOG010000001        55148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_394_length_55148_cov_25.888661, whole genome shotgun sequence.
ACCESSION   DAOOOG010000001 DAOOOG010000000
VERSION     DAOOOG010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 55148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55148
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_394_length_55148_cov_25.888661"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PK242_00005"
     CDS             complement(<1..584)
                     /locus_tag="PK242_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ92440.1"
                     /translation="MGQPLHADRAPRLGVSGRIAAYFQSAQITPLLALVAFLLGLFAV
                     LVTPREEEPQIDVTMANVIVPFPGASARDVEAMVATPAEHVLSQMSDVEHVMSVSRPG
                     VAVLTVQFKVGVPRQTAVVRLYDAVGANADWLPRGLGVLPPIVKPKGVDDVPIVALTL
                     SSARQDSGAFDLERVAHSLESELKRVPGTREVYTL"
     gene            complement(584..1606)
                     /locus_tag="PK242_00010"
     CDS             complement(584..1606)
                     /locus_tag="PK242_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ92441.1"
                     /translation="MLLLAACGALVLPVSAQELPHMTVNAQAARSAQAFEGTVEAVRQ
                     SVLAAQVPGAVLELNVKAGDRVRAGQTLVRIDARAAEQGAAASQAQVVAARAALEVAA
                     AELARKRQLYERNYIAKAALEQAEAAQRAAQAQVNALQAQAGAARTQTAFHVVTAPFD
                     GVVAAVSVERGSMAMPGSPLLTLYDPAALRVSAAVPSAVVAAGVQQARIELDRARAPI
                     HPTRVQVLPTVDPGTLTQQVRAELPVGLEGVAPGLFARLWLPGTGGADAAAPQRLFVP
                     RTALVQRAEMTGLYVLGEQGQPLLRQVRLGPVQGDQVEVLAGLSAGDKVVTAPQAAVR
                     LAPAGR"
     gene            1779..2141
                     /locus_tag="PK242_00015"
     CDS             1779..2141
                     /locus_tag="PK242_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOZ92442.1"
                     /translation="MQQLPPEALEHVAHYFRTLSEATRLRILNVLGHGEMSVGDIAEK
                     VDSSVANVSRHLAQMAQHGLVARESRGNSAYYRIADPAVHQLCDLVCGNIARRFEDVA
                     STRAAFVRTARAKPTTGR"
     gene            complement(2154..2594)
                     /locus_tag="PK242_00020"
     CDS             complement(2154..2594)
                     /locus_tag="PK242_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ92443.1"
                     /translation="MYKHILVPVDGSTASMAAIDKAAGLARAYGVPVTAIFVIDPYPF
                     TGVGADFAYGQDQYLNAARAEAGAAIEAANQRLQAAGVQVDSRVVESHAVWRGILEAA
                     GAVGADLIVMGSHGRRGLEKLVLGSVAQSVLSHTKIDALVVRAS"
     gene            2786..3439
                     /locus_tag="PK242_00025"
     CDS             2786..3439
                     /locus_tag="PK242_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92444.1"
                     /translation="MPMDTINLALVGISLGLALSFRPWRLLAGGALLSPLLASLVITP
                     WLWALPWLHRMPIQLQLSGACLIGLALGWPLAVPVFCLIAVLVGLIAPVSWAQQLDMA
                     LWLGIVPATLSFGLGMVLRRWVWRNLFVYILGRAFLGTALCMFASGALAQWTGHRLAA
                     AVDPDLAMVARWLIAWGDAFMTGMMAAICVAFRPHWLATWSDWLYIAPPPSPRSNQP"
     gene            3519..3656
                     /locus_tag="PK242_00030"
     CDS             3519..3656
                     /locus_tag="PK242_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HOZ92445.1"
                     /translation="MKLWQDLFGTDYGLMSIAGIIFMIGMAIWFVRFFLRKIAQSSDQ
                     Q"
     gene            complement(3672..4289)
                     /locus_tag="PK242_00035"
     CDS             complement(3672..4289)
                     /locus_tag="PK242_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOZ92446.1"
                     /translation="MWGWFVSRFLSAPAAAAKVPTPPEARARLTSRPGDAPVQAALSA
                     QVPAPRRAAEQRRLAALQARGDDAHAYLVETALRICRMPLGAMALRDGPWLRFKAQAG
                     VAVEALPLPGSPCELAMRCADQVTVIEDLGGDPRFSGCALLAQGLAIRTWAGAPLVGA
                     SGKVWGVLWVADRRAGPLSPVQLRTLQLLARQTALLLEAAEAPRG"
     gene            4407..6809
                     /locus_tag="PK242_00040"
     CDS             4407..6809
                     /locus_tag="PK242_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOZ92447.1"
                     /translation="MGLRLLRHMAIGLAGMLALGAMAAPAFDQVRADFRPSETVLLDR
                     SGQPLHRLRTDARVRRGDWVRLDQVSPALRQALVLSEDKRFYEHSGVDWHAVGAAAWA
                     NLWHTRTRGASTLTMQLAGLLDDDLRLGSGGRSLRQKVGQVVLATQLEAQWRKDQILE
                     AYLNLVPWRGELVGIDALSRTLFAKAPHGLQDGEAAIAAALVRAPNAPAAQVARRACG
                     VLQSMRGDRAAADCVALDMQTEAALARRAFAPSDGIAPHFARRLLAATRNGSDRAPAT
                     LPSTLDGRLQRLALEALQGQLRELRGRHVADGAVIVLDNASGEVLAWVGSSGALSSAA
                     EVDGVLAPRQPGSTLKPFLYAQAIAERRITAASLLHDSPAQLHTASGLYIPRNYDLQF
                     KGWVSARTALAASLNVPAVRVGVMVSPRAFAQQLGRLGIELAESGDYYGYSLALGSAE
                     VSLLQLTNAYRALANGGRLCPVRLLPNAPGSLPLPLGEGRGETETHSRAASLSLPLPS
                     VGEGRGEGNRSPQVTATTPCTQALDAGAAFITSDILSDRNARAPTFGTDSILATRFWS
                     AVKTGTSKDMRDNWAVGYSRRYTVGVWVGNASGAAMWDVSGTSGAAPVWATLMRELHR
                     HTPSPPPAPPAGLQHVAVHYAAGLQAPRSEWFLPGTAQSEFAIETEAASADPAKAGGQ
                     KGLKTASHPSAPAGVPPRITAPQDGTILALDPDIPPAHQRLRLAAEPGSAPANSLRWW
                     VDDQPIGRGAQADWLPMPGRHRITLCDGRGRVVDERRIEVRGAQPRHPRSAGAADRLA
                     KQ"
     gene            6829..8286
                     /locus_tag="PK242_00045"
     CDS             6829..8286
                     /locus_tag="PK242_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOZ92448.1"
                     /translation="MFEQTFKNIDDILHKDAGCTSELDYTEQSSWLLFLKYLDDLEAD
                     KAAEAELAGKPYRYILDAPYRWSSWAAPQDAAGALDHNSALTGDDLRDFVNQRLFPYL
                     AGFKARASGPNTIEYKIGEVFGEIKNKLQSGYTLRDVIEQVDALRFRSQTEKHELSHL
                     YEAKIRNMGNAGRNGGEYYTPRPLIRAMIQVTKPRIGERIYDGACGSAGFLCEAFDYL
                     RSQPGLTTADLQTLQQRSFYGKEKKSLAYVIAIMNMILHGIEAPNIIHTNTLAENLAD
                     IQDQDRYDIVLANPPFGGKERKEVQQNFPIKTGETAFLFLQHFIKSLKAGGRAAIVIK
                     NTFLSNTDNASVSLRKLLLEGCNLHTVLDCPGGTFQGAGVKTVVLFFEKGAPTRSIWY
                     YQLDPGRSLGKTNPLNDADLAEFVTLQASFADSAQSWSVDASRIDAASCDLSVRNPNG
                     GEAVALRSPHEILDEIAALDAESAEALARIRELVA"
     gene            8283..9524
                     /locus_tag="PK242_00050"
     CDS             8283..9524
                     /locus_tag="PK242_00050"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOZ92449.1"
                     /translation="MNGRWPVRPLGSLCRIARGGSPRPIQAFLTDTDDGINWIKISDA
                     TASGKYIYSTVEKIKPSGVSRSRMVKEGDFLLSNSMSFGRPYIMRTMGCIHDGWLVLS
                     GYQNFFDQDFLYHLLGSPQVFQQFNQLAAGSTVRNLNIDLASRVQLPIPSLPEQRRIV
                     AILDEAFDAIATAKANTEANWQGARAVLNQCRDDALSSLAAEFGTVNLGSVCDFENGD
                     RGKNYPGKQHRVAEGVPFINAGHLTEDGIDFESMDYISPERYSLLGNGKIKPRDILFC
                     LRGSLGKFANVGDTPVGAIASSLVILRPRSPLYDKYLEIYLASSCCIEMINKYKGGAA
                     QPNLGAKDLKQFVLPLPPQSLQRQLTERLDGIKEQTHLLEAIYSEKSAALDELKKSLL
                     HLAFTGQLTARAADRQLQAVA"
     gene            9521..11827
                     /locus_tag="PK242_00055"
     CDS             9521..11827
                     /locus_tag="PK242_00055"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOZ92450.1"
                     /translation="MNEAETRAEHIDPALAAAGWGVVEGSRIRREVIAPGRLQGRGQR
                     ARADIADYVLIYRNTKLAVVEAKAWDKPHTEGLAQAKRYAAKLALRFAYATNGQQIYG
                     VDMATGAEGDVPAFPGPDALWAQTFAQANAWRDRFAAVPFEDKGGAWESRYYQEIAIR
                     RVLEAIAGGQSRLLLTLATGTGKTAIAFQIAWKLYHSRWNLGDWQAPGAPTRRPRILF
                     LADRNILADQAYNAFSAFADDALVRIAPGEIRKKGRVPRNGSVFFTIFQTFMSGPGGA
                     PHFGAYPPDFFDFIVIDECHRGGASDESQWRAILEHFAPAVQLGLTATPRRRSNADTY
                     AYFGEPVYTYSLKEGINDGYLTPFKVRQFATTLDDYTYTPDDAVVEGEVVPGKRYVEG
                     EFNRVIVIREREQYRVGLFMGMIDPREKTLVFCATQEHALMVRDLINQAKTSPDPNYC
                     MRVTADDGAMGEQFLRDFQDNEKSIPTILTTSQKLSTGVDARNVRNIVLMRPVASMIE
                     FKQIIGRGTRLFDGKDYFTIYDFVKAHHHFSDPEWDGEPVEPEPPRPRAPAPTADPGE
                     PPAVTEVPPAYRIRIKLADGKARAIQSMTATSFWGADGRPLSAAQFLEMLFGALPEFF
                     KDEDELRALWSDPATRRRLLDQLAERGFGREPLAEMQKIIEAEHSDLFDVLAYVAFAT
                     QPRTRAQRADAAKAAAAPGLSDKQQAFVDFVLAQYVQQGVDELDQDKLSPLLRLKYRA
                     LPDAFAELGRPEQIRGVFVDLQRHLYLD"
     gene            11961..12263
                     /locus_tag="PK242_00060"
     CDS             11961..12263
                     /locus_tag="PK242_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HOZ92451.1"
                     /translation="MRVIAVSTLRAFWQRHPDAEQPLNAWYEEATSATWTQPADIKAR
                     YRSASVLKNRRVVFNIKGNDYRLIVAIAYRLQIVYVKFVGTHQEYDAVDAETIESA"
     gene            12282..12653
                     /locus_tag="PK242_00065"
     CDS             12282..12653
                     /locus_tag="PK242_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOZ92452.1"
                     /translation="MDIRPIRTEADYQATLKEISGLMACDPELGTPEGDRLDILATLV
                     QAYEAKHFAIGAPDPVEAIKFRMEQSGLSVKDLEPLIGKSNRVYEVLNRKRPLTLAMI
                     RRLHQSLGIPADVLIAETAAG"
     gene            complement(12926..13678)
                     /locus_tag="PK242_00070"
     CDS             complement(12926..13678)
                     /locus_tag="PK242_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ92453.1"
                     /translation="MDRSTAGAQSLRRGLQLLRLLAEHQEDGIRLSEAMAASGLQRST
                     CHRLLACLVEEQFAERDAGGKVYRLGVDAMQLGFAAMRRVPLVDQCRALMQRLARMSG
                     DTVFLVIRQGDHCLCLHREEGPFPVKVFTTDIGGRRLLGLGAGGLAIMARLPDAEVLR
                     IYERNAAEYAQADFTRERLLRAVHQTRAQGWADITDTLTAGVSGVGVTFAAAPGAIAA
                     LSFGAITPRLPIERKRELARLLRQECPAVAGG"
     gene            13774..14577
                     /locus_tag="PK242_00075"
     CDS             13774..14577
                     /locus_tag="PK242_00075"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOZ92454.1"
                     /translation="MAFDTLAIETHGAVRRILLARPAQRNAQNWQLLDELMAALDQAR
                     TDDAIHAVVLGGQGEHFSAGHDLKQAQAERANFTVEQRWAFEELRYFDYSMKIWDFPK
                     PTIAQVQGACVAGGFMIANMCDLVVAGESAYFSDPVGHTLGAAATEVLIHPWVMGARK
                     AKELLFTGARMSAQEAQAIGMVNRVVPDAELADATLALAQQIAKAPPFGLRLIKRSIN
                     RTLDAQGLRSALAAHFDTHQLSHLSDGFLSARDRGLASAIQKNPAKAQQ"
     gene            14583..16670
                     /locus_tag="PK242_00080"
     CDS             14583..16670
                     /locus_tag="PK242_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOZ92455.1"
                     /translation="MHRLQPLLDPASIAIVGASNNPARIGGMPIAHLTKFGYAGAIYP
                     INPKYQEVFGLRCWPDIEALPAAPDLVVLAIAAPEVLPMLRRCAAKGVRAVIVYAAGF
                     AEEGERGAALQAELAAYVASTDMVVAGPNAMGFANLNTQAHTNFASVFNTAPMQAGTG
                     SVSLLTQSGNVCAAVYAIARRLGVAFSHFINTGNEACVDFAQYLEYLAQDEATDVCIG
                     YIEELRDGARFIAAAEEFARRDKLLILYKAGETDKGSEAVRSHTSALAGDQAIYRAAF
                     RQLDIIQSHDFVQMAQLAHLASYRRRRAGKRVAIITISGALGAILADKFIGAGLEVPT
                     LPAELQQQLRAGIPDYGMVANPVDVTGNVVNDPGFVRTALEALATSDAVDTVVIYAPG
                     YMLDRMAGDLCAVAAAHPRLFVAIDTGAAQSREQLRAGGVPVFEDIGVATAALAPFLL
                     WQQQRQRNRWRALRHQAPPPATALPEVLDEHQCKAVLAASGVPAAPERRARSADEAVA
                     AADALGYPVALKVLSPDIAHKTEAGGVRLHLADGAQVRAAFDQILASARAHAPQARLD
                     GVLVAPMQSGVAELIVGATRDPVFGMALTVGLGGVLTELYQDVSHRLLPVDEAMAADM
                     LAELKAYPLLTGYRGRPPADVDAACAAIAAFSRAVQALGRQVSEAEVNPLLIKASGQG
                     AAMLDALLLAPAD"
     gene            16705..17670
                     /locus_tag="PK242_00085"
     CDS             16705..17670
                     /locus_tag="PK242_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ92456.1"
                     /translation="MIKQLLAGVALAAAALCTPAQAQGYPSKPVNIVVPFAPGGATDI
                     MTRLLAERLSKRLGQPVIVENKPGAGTMIASEYVAKAPADGHTVLMAASSLGIAPSLY
                     SKVNYDPVKSFAPISLVASVVHVLEVHPSVPVKSVGELITWLKANPGKANYGSVGAGT
                     STHLESELFNTMAGVKMVHVPYKGSAPALTDLVGGSLQVMFDAWASSGPFVKDGRTRL
                     LAVTTAQRSKILPDVPTVAESGLPGYEAMPWLGLLAPAGTPDAVVKKFHDELMEVLKE
                     QAVQDKFHSLGLDIIGNTPQEFADFIKKDITKWAKVIQETGAKAN"
     gene            17754..18923
                     /locus_tag="PK242_00090"
     CDS             17754..18923
                     /locus_tag="PK242_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ92457.1"
                     /translation="MQVGYAAEDEAFRAELRAWFETHYPRFVAAWPQAPDPNALDWRR
                     AWEDYVCQAGWSGLGWPLAYGGKAWSLTRQAIFHEEQARIGAPLGVNLIGHGILAPTL
                     IHFASEAQKHRYLPGILSNREVWCQGYSEPGAGSDLAALGTRAERRGDHYLLNGHKIW
                     TSFAHIADYCFVLARTDPAAQRHKGISFLLVDMKSPGVRVQPIRQITGEADFNEVFFE
                     DVRVPLDALVGEENGGWRLAMAAASFERGTYFIPRQVRFAQEVKALQRLAIDTGADRD
                     AHLRREVTRLALDSHLLRLKGLRALTLALRGGPPGAEGSATKLHWSEAHQRLTSTAAE
                     LLGERALAGPAPGGHDPAAALWAREFLWTRAETILAGTSEVQRNILAEQMLGLPR"
     gene            18928..19989
                     /locus_tag="PK242_00095"
     CDS             18928..19989
                     /locus_tag="PK242_00095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ92458.1"
                     /translation="MDFALNQAQQMLQASARRFFADHHPLTRARQALPWSDAAQQRLW
                     ADMADMGFLALLLPEDQGGLALGLTEASLLAEEAGRQLLNLPWAASAVWLALACRAAP
                     ASAPAPLRDAVQAMLAGRRALHVVDAGDGWWDHAGQCSDIVVIRESPAPGEPLQLALI
                     DGAAVSAAPALDPTLRQAPALAPAGPWHDWALDAATRAQARTGYRLMRAAELVGVAQA
                     ALELAAGYARERVQFGRPIGSNQAIKHQLANAWMGVDNARLAVLYATAALNERLPDAR
                     LACAAAEASAIEGALQATRSALQVHGGMGFTWESDVHLYLKRAQHLATRLGGASRALQ
                     QLEAQVWDAAANAPSATPV"
     gene            complement(20210..20971)
                     /locus_tag="PK242_00100"
     CDS             complement(20210..20971)
                     /locus_tag="PK242_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOZ92459.1"
                     /translation="MNNTLIKGLRLLEVLVARAQPVGISELAQELEMGASNVHRLLQA
                     LVELGYAVNEGGRGGYRASLKVWELGAQALHKLDFRETAAPAMRMLLAETNETVHLSV
                     LSGDEVIYIDKLDSPEPVRAYSVIGGRAPAYCVATGKALLAWREQPAVQLMTVRPLQV
                     YTPTTLADTSALAMELDRVRQQGYAVNRGEWRASVWGIAAPVFDERQRVVAALGISGP
                     AERIRARGVKRLAELVVQAARQASSRQLALAGEAR"
     gene            complement(21200..22027)
                     /locus_tag="PK242_00105"
     CDS             complement(21200..22027)
                     /locus_tag="PK242_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOZ92460.1"
                     /translation="MLWKTLKVPEIETLPGPVFVRAQDIPPGYRFPSHSHPWHQLIYA
                     ISGSLVVEAAHQRVVCGPEQAVWIPTGTVHAVASRYGAQFRSLYLRTSVGRGMAARYA
                     LLQVSALLRELIREAAWLGEQRVAGVATLAYTQRVHGLILDQLPRLERVQAPLPWPQS
                     PMLRKLCEAIDRAPADADDIATWGARLGASERTLTRRFQAELGMNFREWKRRLRLFKS
                     MELLGAGMSVTATALELGYGSPSAFSYMFTREMGCSPKTYRAAPGRERQRSARSRAR"
     gene            22119..23522
                     /locus_tag="PK242_00110"
     CDS             22119..23522
                     /locus_tag="PK242_00110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ92461.1"
                     /translation="MRDTEAWSTTPRAMPADIEPPRRIGFAATALFIYLILLAGYVLS
                     YVHRTAPAAIAGELTQAFQVSGALLGTLAATYFYVYTLLQVPVGVLADTVGPRVVVTA
                     GAVVAAVGSILFGLAPGWELAAVGRLLVGMGVAVTFVALLKVCANWFPPQRFATFNGI
                     TLLAGNLGAAAAGAPLAWLVTVASWRSVFVGLGLASLALAVLTWIVVRDSPRECGFEV
                     SYAPPPAVATHWAQALRAVLANPASWPGFFVNVGVAGSFFAFAGLWVVPYLREGRGLS
                     QAVASHHASLLVLGVAAGSLVVGLLSDRLRSRIGVMRLFALMYALSWLPLVLRWEPSL
                     ALSYAWFFVMGLFVPAFVLTWTVAKEMNPPEHAGMAVSLVNVGIFLGAGILQPLAGAL
                     LDAVRPVLGPTAAWDRAIWLFAASAAAGALCTLFVRHEYPAPAPAHSRRQTGAAATAA
                     RPGAGHGSVCHSKRGNP"
     gene            23642..25981
                     /locus_tag="PK242_00115"
     CDS             23642..25981
                     /locus_tag="PK242_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HOZ92462.1"
                     /translation="MTASHWGVYEATGQGEALRLQGFARDPSPSPIGLSMLDAARGPV
                     RVRRPAVRRSWWQAMCGDAPHAAPQLRGTDPFVEVGWNEAIDLVARELDRVRLQHGNE
                     AIFGGSYGWSSAGRFHHAQSQVHRFLNAIGGYVRHADTYSLGAARVLLPHIVAPMEEL
                     FTQHTSWRVMARHTRLFITFGGVPAKNAQVSSGGATEHAVPTGLRRMAAAGVRFINIS
                     PVRDDIDTGGEVEWIPIRPGTDTALLLAMAHTLWTEKRHDAGFLASHCVGFERVAAYL
                     RGDTDGQPKSADWAARITGVPAERIVRLAREAAATRSMLNIAWSLQRSHHGEQPYWAL
                     ITVAAMLGQIGLPGGGFGVGYGSTNMMGNDNAKFSGPTLPQGHNAVRAFIPVARITDM
                     LEQPGASFDYNGQRHTYPDIRLIYWAGGNPFHHHQDLNRLLAAWQSKPETIVVHEQYW
                     TPTAKLADIVLPATTAMEREDIGYATRERYMVAMKRLIAPVGEARDDYAIFSDLAERL
                     GVREAFTEGRDARDWLSLMYEQSRQRAAQAGIELPPFDDFWTRGLLDLAQPEAEVVML
                     DRFRADPQSHPLATPSGRIELFSARIASFGYDDCPGHACWLEPLEWLGSPAAVRFPLH
                     LISDQPHTKLHSQLDHAACSQANKVDGREPLTLCRTDAEARGLKAGDLVRVFNARGAC
                     LAALRISDALRPGVARMSTGAWFDPTSWLASGPGNVERHGNPNVLTPDIGASRLSQGC
                     MAQTCLVEVERCTSPETRPTPYLLPEFVGTAHPHEPACT"
     gene            26030..27631
                     /locus_tag="PK242_00120"
     CDS             26030..27631
                     /locus_tag="PK242_00120"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HOZ92463.1"
                     /translation="MNTPVCDYLIVGGGTAGCVLANRLSADPSCRVILLEAGGPDDDK
                     WIHIPAGIRYLLREKKHNWFYMTEPSPLTNGRSVYWPRGKVLGGSSSINGMVYIRGQR
                     TDFDRWAASGATGWDWDSLFPYFLKIENQTRGADAYHAVGGPLTVSDRTNRSEVWTRF
                     IAAAEQLGIPRNPDFNGADQEGVGYYQATVGNGRRCSAAVGYLRPVKGRANLSVITGA
                     MVHHISFEQRRATGVRYEKDGHWHELHAAREVLVCGGSINSPQLLMLSGIGPAAHLQE
                     MGITPRVDAPQLGKNLQDHLQLRLTYRLNRPISFNDQFHSLAGKVGMGLEYLLRRSGA
                     IAYPTAQVGLFTRSLPELASPDIQYHFSNYTTDEKTGMPDRFPGMTFSVCHLRPESRG
                     EILLRSPDPGQHPRILPNYLATPEDCRVAVEEVRLTRRLAATQPLADIVAEELAPGPD
                     VQSDEACLDFARSNGTSIYHPVGTCRMGSDAEAVVDPALCVRGVSHLRVVDASVMPTL
                     ISGNTYAATLAIAERAADLILNTRP"
     gene            27667..28629
                     /locus_tag="PK242_00125"
     CDS             27667..28629
                     /locus_tag="PK242_00125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ92464.1"
                     /translation="MKLTPILTGLSACLMAAAAHAQSVDKYPERPIKIIIPYQAGGGT
                     DVMTRIIVEKVRERTKQPFIIETKPGGNTVIGVKALLAAPADGYTLMMTSSATFHTAP
                     IIVKNPGYDPIESFSLISYIAYTPVVFVINSSVKANNLKEFIDLAKAAPGKYALSTYG
                     RQLGTDLFQKVAGFKLNSIPYKGVEAVNAVVAGHTDAMFDGVFTALPYLKQGKTRALA
                     VMQDKRTRFAPDIPTVTELGYKSMPDIPIWYGLVGPKGVDQKIIDKLANEFKIAMESP
                     EVIEKLANLSVESVGSGPQALRKQITAEYGHYRKIADELGIQPE"
     gene            28673..29653
                     /locus_tag="PK242_00130"
     CDS             28673..29653
                     /locus_tag="PK242_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ92465.1"
                     /translation="MTHPDRLRLSLPLLLAAPQLLWSVPARAQDKWPSGPIRMVVPFP
                     PGGSSDMLGRLMADKLSALLKSNIFVENKPGGTTQIGTELVANAAPDGHTLLMASATG
                     FTALPNLRKLNYGIDSFEIAGGVADYVAVMAVRKTLPIRTVKEFIDYAKQHPGKLSFG
                     SAGEASAGHIYGLTLARDTGIQVLHVPFRGSAAAVNALVAGDVDFIIDGAVTPMVKAD
                     RVQPLAVLYRRRHPELPGVPTLEEAGFTISSTRGSGWGVLAPKGTPAAIMSRLSEAIR
                     QVLQQQDVKDALARANSVPSWQAPDEYRRSLDADRKLYAELLPAIGIRGN"
     gene            29664..31793
                     /locus_tag="PK242_00135"
     CDS             29664..31793
                     /locus_tag="PK242_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOZ92466.1"
                     /translation="MVDVSNPAPWVAPRQSVRDILHPRSVAIVGASDSEDKWGGRLLR
                     YMLRHRHPGALYPINARATELMGLPAYPSLRDCPGPVDLAILLVPRAHVRAAIEDCVA
                     KGVGCALCITAGFAETGAAGRADEQALVDLARAGGVRLIGPNCMGLMNTHHGLCATTG
                     VVMGTLDRLPVGGIGMASQSGALMGAMISRGVDIGAGFSTTVSVGNQADLDLNDFFEY
                     LIDDPKTEVITLYMEGVKDAPRFTALLERAAAAGKPVCIAKSGRSAAGAKAAASHTAS
                     LAGAWPAFEATCRRWGVYLFETIYDLLQGALLLQRGKRAASDQVAVFSGSGGGGALLT
                     DGLEAHGLAPAELSDATQQAVAAVLPSTHRQLPFDFGMLNHAAAPDPQVAGGAIGIAL
                     DRALHDPAVGAGILLLTTQPGQELVVQAAIAAADRSSKPLLFVQGAGNHGDVARQMLR
                     AAGHGYFDSPHDALKVLSVLARRKPPPPSAPPQPGVSVAADLPAGFLTEPQARRLLEG
                     AGIPTTRWRFAAGADAAAQAARELGGSVAIKAVSAHIVHKSDMGGVKLGLQGDAAVRQ
                     ACADIAAAARAAGVDTLDGYLVTEMWRADTELILGVQRDPDFGPLVMVGAGGVLVELM
                     KDVQLAPAPLSHATALAMLRQLRCLPLLSGYRGRTPADLDAIADVMVRLGALAADPSA
                     RLRELDINPLFIAGPRIAAADARATLA"
     gene            31831..32571
                     /locus_tag="PK242_00140"
     CDS             31831..32571
                     /locus_tag="PK242_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOZ92467.1"
                     /translation="MTAVRYASEGGIARITIHRPEARNAINDEVVQGMQAAWQRFAQD
                     ADDRVAVLHGAGEQAFTSGADLKALPRDIWLAMPNLSVPCDKPIIAAVSGLAVGAGAT
                     MVMLADMAVADEDARFVYPEARIGAFAGVMAGFPPRMQYKAGLEWLLTGDPVTAQRAY
                     EIGLVNRLAPHGQALEVAMQVASRIAANAPLVVQAMKSIARRTLPASPTELYYPQHRM
                     LQGIAASADLREGVASFKEKRAPRFSGR"
     gene            32603..33580
                     /locus_tag="PK242_00145"
     CDS             32603..33580
                     /locus_tag="PK242_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ92468.1"
                     /translation="MTLTRRSLHLLALAALSGAVAPALAQTSKPWPDKPIRFVTPYPP
                     GGSSDVITRFIGDGVSKILGQPVIVENKPGAAATMGTEFASRQAPDGYTFLVAPTAAL
                     ALAPYLMKNVKYTFESFDPVAKIASSYGLVTARKDAPFTDYKGMLAYAKANPGKLTFA
                     TNGVGSIVHMTAVLLHKHAGVQLTHIPYKGAGESVIDLLAGRIDLMYDPATAPRVKSG
                     DLKGLGTTSGIRNPELPNLPTLKEQGFGDGGSFSWFAMFAPKGTPAPVVNKMADAVRQ
                     VLEAPEVKAKLQLSALYPNYEDPATFAKSVRNDAETLRNVIQQEGIKLE"
     gene            complement(33751..36411)
                     /gene="mdeB"
                     /locus_tag="PK242_00150"
     CDS             complement(33751..36411)
                     /gene="mdeB"
                     /locus_tag="PK242_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate dehydrogenase"
                     /protein_id="HOZ92469.1"
                     /translation="MHAPIDSQALASSASLPADADPQETAEWRAAFEALIATQGPARA
                     CFMLDELARLARARQLGWRPELNTPALNTIAAQDQPVFPGDLAIEERLGSLIRWNALA
                     MVVRANQAYGELGGHIASYASAADLFEVGFNHFFRAREGLAPGQHRGDLVFFQPHSAP
                     GVYARAFLEGRLSEQDLLHFRQELTAPGAGARGLCSYPHPYLMPDFWQFPTGSMGIGP
                     ISSIYHARFMHYLTDRGLLDCAGRKVWGVFGDGEMDEPESTSALTLAAREGLDNLVWV
                     VNCNLQRLDGPVRGNGRIVDELERLFTGSGWNVIKLLWGSDWDGLFARDLTGTLVRTL
                     GETVDGQMQTFAAKDGRFNRDNFFGQSPELQQLAQGMTDEQIDRLKRGGHDLVKIHAA
                     YAAAHAHRGQPTVILAHTKKGYGMGAAGQGKMTTHSQKKLDEQELIAFRNRFNLPLTD
                     AQAANLEFLKPAVDSAELQYLLQRRRALGGWLPARQTLCEAVAVPALTSYAQFAVAAD
                     GKDMSTTMAFVRMLGNLLKDGQLGPRIVPIVADEARTFGMANLFKQVGIYSSVGQRYA
                     PEDIGSILSYREARDGQILEEGISEAGALASWTAAATSYSVHGLAMLPFYIYYSMFGF
                     QRVGDAIWAAADQRARGFLLGATSGRTTLGGEGLQHQDGTSHLVAATIPNCKAYDPAF
                     AGEMAVIVDAGMREMMVEQKDVFYYVTLMNENYAQPALPADAHPGVLRGCYLFSSYRR
                     TPDGRELPETAPTVTLLGSGAILTEVILAAELLVRQGIAATVLSVTSWSELARDGLAC
                     QQRRLAGADTPGAPWVTQQLHHTQGPIIAATDYVCAVPESVRAFVPEGRSYLTLGTDG
                     FGRSDTRAALRRFFGVDARALVRAARLALG"
     gene            complement(36540..37793)
                     /locus_tag="PK242_00155"
     CDS             complement(36540..37793)
                     /locus_tag="PK242_00155"
                     /EC_number="4.4.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018826 - methionine gamma-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase"
                     /protein_id="HOZ92470.1"
                     /translation="MTRSLDRAGGFSTRAIHFGYDPAEHLGALVPPIHTSATYAFPDT
                     GYGARCFTGEEKGYIYSRIANPTLALLEARLASLEQGAGAVVFASGMGAITATLWSML
                     TPGDEVLADLTLYGCTFSFLHHGIGHFGVTVRHVDMTDPEQVAAALTERTRVVYIESP
                     ANPNMRLADIAAISRLSHARGARVVVDNTYCSPYLQQPLVLGADVSLHSVTKYLGGHG
                     DLMAGAAVFADAELAERVRLYGLKDMTGAVMSAHDAHLVMRGLKTLALRMDRHCQNAQ
                     QVAERIAAHPAAAVVHYPGLPSFAQHALAQRQMRQMGGMIAFELHGGLQAGVRFMDAL
                     RLVTRAVSLGDAETLAQHPASMTHSTYTPEERAAHGIPEGLVRLSIGLEDVEDILADI
                     GQALDHALAPEGAARGGARSLSSVF"
     gene            37927..38406
                     /locus_tag="PK242_00160"
     CDS             37927..38406
                     /locus_tag="PK242_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOZ92471.1"
                     /translation="MSSPSGSFDHVDRALLEVLQRDGRATVGQLAERVSLSPSPCWRR
                     VRQLEESGVISGYHAHLDRRRIGYGVLGFVQLNLHDHTSSICAAFEREVLALPQVLAC
                     HNLSGRHDYQLEVVAPDLEYFAEFVRSRIRSLPGVREISTSFSLKEIKRTIALPLDH"
     gene            38472..39860
                     /gene="gorA"
                     /locus_tag="PK242_00165"
     CDS             38472..39860
                     /gene="gorA"
                     /locus_tag="PK242_00165"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HOZ92472.1"
                     /translation="MTDFDFDLFVIGGGSGGVRAARFAAQRGARVALAESGRMGGTCV
                     NVGCIPKKLYSYGAHYAEAFEEARGYGWQLPGAPRLDWDALKARRAQEISRLNGIYEG
                     LMTGAGVRRLQGHGRLLDAHTVDVTAPDGGRSRHRARHILLATGGTPVRPELPGHELI
                     VTSDDMFDLPRFPRHLVVVGGGYIGCEMASIFHGLGAQVTLLYRGEQILRGFDDEVRD
                     FTAAELRKAGIDLRVRTEVTRIEAAPDGTRRLTLNDGGVLQADVVLYAVGRRPNVAGL
                     GLEAVGVRMTASGAIAVDEHFATAVPGLYALGDVRGGLELTPVALAEAMALVDHLFGP
                     LPGQARRTMDYEHIPTAVFTHPPIGTVGLTEAQARARVGTIRVYRSDFRPLKHTLSGR
                     DERCLVKLIVDDASDRVLGLHMVGADAGEIVQGFAVALKCGATKAQFDATLGIHPTSA
                     EEFVTLRDVTRT"
     gene            complement(39908..40339)
                     /locus_tag="PK242_00170"
     CDS             complement(39908..40339)
                     /locus_tag="PK242_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92473.1"
                     /translation="MKAKLHGVFGLVALLCIVTFWLSTVVAELLLDQASVVFVKNMVV
                     AGMWVLIPAMAATGGSGFSLARSRRGRLVEAKQRRMKMVAVNGLLVLLPSALLLARWA
                     SAGRLDAGFYALQGLELVAGATNITLLALNLRDGRRLAGRR"
     gene            complement(40336..40806)
                     /locus_tag="PK242_00175"
     CDS             complement(40336..40806)
                     /locus_tag="PK242_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOZ92474.1"
                     /translation="MNALPQIDAISALALAVFRLNGALLHWGDRRVATLGLSSARWQM
                     LGALALADSPLSAPQVGAAMGVSRQGALKQLGVLQGLGLVQVDPNPAHRRSPLYCLTP
                     HGRKCYEQADALWRDQVAQLARQISATQARQATEVLQHIEHVLGSATADLEDAT"
     gene            complement(40864..42213)
                     /locus_tag="PK242_00180"
     CDS             complement(40864..42213)
                     /locus_tag="PK242_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOZ92475.1"
                     /translation="MRVIAGDAWAVFVGQVAVMGFAVVDTIVAGRHSDLSLATLSIGA
                     AVFVTVHVSLMGLFTALLPIWAELRGARRLGEVGGSVRQALYIWAAASLLGLAVLLQP
                     EPLLRWTDVPAGLQPVVVDYLAVLGWSLPAALLFRAFGTLNQALGRPQLVSWLQVLAL
                     AVKLPLSVWLTFGGAGVPALGVVGCAWATFAVHGLLLGLALWLLRTQPLYEPLQLWRR
                     PEPPHGPTLAAFARLGVPAALTMLVEITSFTLMALFVARLGTTATAAHQIAANVAALM
                     YMAPLSLAVASSARVGWWRGAGDEARARAVVMQSFWLAGGLGLALAAMVFVAKPWIAA
                     VYSAQAGVVLLATRLLGWVALLHAADALQTVAMFLLRCYRVTLAPFVVYGVLLWGVGL
                     AGGYLLAYHGVGPLAAQHSPVAFWQAGGGALIVVALLVWLMLGRVSRRAAASVSVTH"
     gene            42404..43567
                     /locus_tag="PK242_00185"
     CDS             42404..43567
                     /locus_tag="PK242_00185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HOZ92476.1"
                     /translation="MTDLSKITCIEDLRVLAKCRVPRMFYDYADSGSWTEGTYRANQA
                     DFAPIQLRQRVAINMEGRSTATTMIGEPVAMPVALAPTGLTGMQHADGEILAARAARA
                     FGVPFTLSTMSICSIEDVAQHAGPGFWFQLYVMRDRDFIERLIDRAKAAGCGALVITL
                     DLQILGQRHKDIKNGLSTPPKPTLANLINLATKPRWIMGMLGTPRRKFGNIHGHVKGV
                     SDMSNLGAWTADQFDPRLNWGDVEWIKKRWGGKIIIKGIMEPEDARLAVDSGADALIV
                     SNHGGRQLDGAPSAIAALPAIVDAVGTQIEVHMDSGIRSGQDVLKAWALGARGTYIGR
                     AFLWGLGAAGEAGVAKALQIIHKELDLTMAFCGHTRIESVDRRILRPGTFPTP"
     gene            43581..44534
                     /locus_tag="PK242_00190"
     CDS             43581..44534
                     /locus_tag="PK242_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ92477.1"
                     /translation="MLHPQAQALIDLLVAHGLPPTHTLTPAEARAFYRERRALTQPAP
                     PDVAELRELRLPGPAGELAARAYRPLGARPEAVLPVLVYFHGGGWVIGDLDTHDTLCR
                     ELANASGCAVVSVDYRLAPEHPFPAAFDDALAATRWVARHAGELGADPTRLAVGGDSA
                     GGNLAAAVALAARDERDAGGPLPIAFQLLIYPATDMRRGHGSHQRNGQGYILTRETMD
                     YFIGHYLPPGTSPTDWRGSPLLAASHVGLPPALVLTAGFDPLVDEGLDYARALTAGGS
                     RASYVCFGRQIHGFITMGRVLDEANSAVALCATELRRALTP"
     gene            complement(44554..45030)
                     /locus_tag="PK242_00195"
     CDS             complement(44554..45030)
                     /locus_tag="PK242_00195"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOZ92478.1"
                     /translation="MRRWYEHRIPPPVIDLACAAVMAWLAGAVPAARLWPAGGAWWVW
                     ALALGLALIGALVALAGLLEFRRARTTFNPLAPQRARTVVTRGVYRWTRNPMYLGMLV
                     VLAGWAVWLGSAAALLGLPLSVLLLNRLQIGPEERILRERFGQDYIDYQARVRRWL"
     gene            complement(45076..48063)
                     /locus_tag="PK242_00200"
     CDS             complement(45076..48063)
                     /locus_tag="PK242_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOZ92479.1"
                     /translation="MFPFSRESNDPQPAPDSPARPAAEAHPLDALTGGVFSAATSGER
                     AQRVRDWLASGPTPEQMQEVFKELSAKDKGAARALRERLDELRRAKGQEAVAAEWAAK
                     AQALLQAARLNIADALAWQRDAAKAGAPLSREPLAGLRTQLAQRVKVVEDLQQRVMVQ
                     REAAILLAQRIELLSTKPWQDAQAQQVGLAADVAQWRQQAAALLADAQWPSVDLRFPP
                     QMEAAQTQLSAVWDAFGAALAQAQAAAADAAAPLPSVPVWADELRAARGEAPAAAAEA
                     DKPARPRIDPEQRAAAHKAVEAGVMLLEQAVTAGHTKNMHSATQALRQSLKTHGRLID
                     DALEARVHAVLVSAGELEGWQRWSADKLREQLVARAEALLVKRRKKAPAQGAPDAAPA
                     PSQAESGQASATEPTAVPAAPEASASPAAPEQTAPEGQPDAIESTAAGADPASAGAEN
                     DAEPAARPLPATTLAADEQWVPAMGGRKLQEAIRKLRDEWKAADQGGPPNHALWKRFD
                     RAVSTAHAFVDEWIAGVRAQAAEHKAQREALIDEVRAWAASHADAGEGGDWKAVNRQI
                     HQFAERWRNAGHLSEKQFAELQPRWKEAIHAAALPLENMQKHSTARRQALIQEAEALG
                     AAPQLRIDAVRALQQRWQAEAQAVPLDRKHEQKLWDAFRKPIDEAFNRKSAEREKAQA
                     AMSAHDRAVLEAAKALEAANASGDAQRIRAAMAQLEAATRAQPAPGSEEKPAAAPADG
                     APEAIEIEATEVPTDAPDAPAEGAAPAPAPKPAKPLVAMRGDDRPGARKAEPAPAGRP
                     GERRPAGRDGRAGDRREGAAGRAGERGARRFDDARGPRRSDEARGPRLGDAAFRAQRD
                     AVEQAQAQLRKLAAQAHGEALTQLLGAWQARQPDQVPGVQELGRGVTPALRNAWTQAI
                     GGGAGAPAAEALLRLEMAAEVPTPAEHLQARRALQLQLLTRRNDPSPAQTWGQDTAAV
                     LAGPHDDATARRLQTALKGLLRK"
     gene            complement(48220..52443)
                     /locus_tag="PK242_00205"
     CDS             complement(48220..52443)
                     /locus_tag="PK242_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOZ92480.1"
                     /translation="MSGSLLSSPPPEPTRAAVAASPAPPERAWWPPRRWRRRLTIALV
                     GLLALWALAWLALPPLLKWQGEKIASAELGRPVHIGAVVVRPWSLQLALHDLRIDAAP
                     GAPPLLSVARLWADAELQSVFRLAPVIDALRIESPALHLTHLGEGRYDIDDLLARLAA
                     RPAAPEPAAPPRFALYNIELTDGRIDFDDRAAGRQHQVRQLRLALPFLSSLPAARQIK
                     VEPQLAFVVNGSAFDSRAQALPFDASRQTEASLQLKSFDLQPYLAYLPAALPLRLQAA
                     TLDADLRAAFAQAGAPTLSVSGNLALRGLKAVDAQGAERLAFDALELELDELRPLERR
                     LALRSVELRGPRLALHRRADGQLDLLAQGAPDATDSIAAGAHPAGSAGPSDPASASAS
                     AGPAGAADGWRLAIGRLALQGGALDFSDASTSAHGAPAARLQLQHLELAARELAWPPD
                     QPLHWEGTAQLTGAPDAGQGAATLAFEGQATRRQAELQARIDGLPLALGAPYLAGVLK
                     PRLDGQLAARLALHWAAPEQAGAPAQLTLSAEQVTLSQLALTDPDAAPAPARGRRRSA
                     LPDDARLVAAEGIELQRLHLDWPARAVRVARLGLRAPLARLAREADGRWMVERWLRQP
                     LPADAPAPTAAAPAWKLTLDELTLADGILGWHDAAVADGPVQAELTQLTLGLRQFELD
                     GRRPMPLQLAARLRAGRSEPGRLSWRGQLTLSPLALQGTADVQRLPLHALQPYFGEWL
                     NVDLLRADASFKGQLELAQTEQGARVRVTGEASLEELRAHSRPGSVVASASASTPAAG
                     DTTPPATPATPVTSAASVTPAAAAQAPRAALAPVAADRAGARSGGLGEELLSWKLLRL
                     GSVDLRLDPGQPAQLGIGETLLSDFFARVVILPDGRLNLQDVLKSEHAAGGSPGAAPA
                     TPPGAMPAAGEFSGTTHSIAAGAPTAGASAPLAAAPAALAPVVRVGPVRLEGGRVDFA
                     DRFIRPNYSADLSELAGTLGAFSSVAPDGQVQMAELNLSGRAEGSAQLRVSGRLNPLA
                     TPLALDIRGQVSELELPPLSPYAVRYAGHGIERGKLSMDVNYRIQPDGQLTASNQLVL
                     KQLQFSEPVAGAPASLPVRLATALLSDSQGVIDLDLPISGSLNDPQFSLGPVIVKAIL
                     NLIGKAITAPFTLLARALGGAGGEDLSQVAFAPGSARLDARARQQLDRVAKALAERPQ
                     LTLTVVGHARLAPEREGWRRQRLQALLAAERRAEATAAPAASAAANPAEDRSPAPDAM
                     PSGAAGADPAGAGAASDATDAMDAEQRLRLLRRLYRRADVPGRPRNAIGLLRDVPQAE
                     MEALLLAHIEVGQDAMRQLAVQRAVAVKDYLAAHGAPADRLFLGAAQAAADAAAAAPA
                     SAASAATSGSAPAAAPAWTPHAELKLATR"
     gene            53196..54017
                     /locus_tag="PK242_00210"
     CDS             53196..54017
                     /locus_tag="PK242_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOZ92481.1"
                     /translation="MTASTRLAALSSGLRTFAGDVAEGFFEITHNGLALVGVVVVLAV
                     ALLGAHPELRQQGEQQLTEWLTARKVAALGITPELGAIERATAANPRELPREQANLAL
                     WISKKYRVAPEPVAALVAEAYEAGQAAKLDPTLILAVMAIESSFNPFAQSSVGAQGLM
                     QVMTQIHSDKYEHFGGQYAAFDPKTNLRVGVKVLQEYIGRTGSVEMGLKWYVGAANLA
                     SDGGYADKVLAEHARLYQVAKGKPLPAALPGLRAQKPAPASPPEPTAPVEVATLS"
     gene            54212..>55148
                     /gene="glyA"
                     /locus_tag="PK242_00215"
     CDS             54212..>55148
                     /gene="glyA"
                     /locus_tag="PK242_00215"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOZ92482.1"
                     /translation="MFNRSSHTIAHTDPELWAAICAEHQRQEDHIELIASENYASPAV
                     LAAAGTQLTNKYAEGYPGRRYYGGCEHVDVAEQLAIDRVKQLFGAEAANVQPHCGASA
                     NIAAFMAFLKPGDTIMGMSLAEGGHLTHGMPLNMSGKWFNVVSYGLNAQEAIDYDAME
                     KKAHETRPRLIIAGASAYSLEIDFARFARVAKDVGAIFMVDMAHYAGLIAGGCYPNPV
                     PHADVVTSTTHKSLRGPRSGFILMKAEHEKAINSAVFPGLQGGPLMHIIAAKAVAFKE
                     ALQPEFKQYAAQVRKNAAVVAQTLTERGLRIVSGGT"
BASE COUNT         8671 a        20054 c        18423 g         8000 t
ORIGIN      
        1 agggtgtaga cctcgcgcgt gccgggcacg cgcttgagct cgctctccag gctgtgcgcc
       61 acccgctcga ggtcgaacgc accgctgtcc tggcgcgcgc tggacagggt cagcgcgacg
      121 atcggcacat cgtccacgcc cttgggcttg acgatgggcg gcagcacgcc caggccacgt
      181 ggcagccagt cggcgttggc gccgaccgcg tcgtacaggc gcaccactgc ggtctggcgc
      241 ggcacgccca ccttgaactg caccgtcagc accgccacgc cagggcgcga cacgctcatc
      301 acgtgctcga cgtcgctcat ctgcgacagc acatgttcgg ccggcgtcgc caccatggcc
      361 tcgacgtcgc gcgcgctggc acccgggaag ggcacgatca cgttggccat ggtgacgtcg
      421 atctgcggct cttcctcgcg cggcgtcacc agcacggcga acagaccgag caggaacgcc
      481 accagcgcca gcagcggcgt gatctgggcg ctctgaaagt acgccgcaat gcgcccggac
      541 acccccaggc gcggcgcgcg atcggcatgc aggggctgtc ccatcagcgc cccgccggag
      601 ccagccgcac cgccgcctgc ggtgccgtca ccactttgtc gcccgccgac aggccggcca
      661 gcacctccac ctggtcgccc tgcaccgggc ccagccgcac ctggcgcagc agcggctgcc
      721 cctgctcgcc cagcacgtac aggccagtca tctcggcgcg ctgcaccagc gccgtgcgcg
      781 gcacgaacag acgctgcggc gcggcagcat cggcgccacc cgtgccgggc agccacaggc
      841 gcgcgaacaa ccccggcgcc acgccttcca ggccgaccgg caactcggcg cgcacctgct
      901 gcgtcagcgt gcccggatcg accgtcggca gcacctgcac ccgcgtcggg tggatcggcg
      961 ccctggcgcg atcgagctcg atgcgcgcct gctgcacgcc cgccgccacc accgcgctgg
     1021 gcaccgccgc gctgacgcgc agcgccgccg ggtcatacag ggtcagcaag gggctgccgg
     1081 gcatggccat cgagccacgc tcgaccgaca ccgccgctac cacaccgtca aacggtgccg
     1141 tcacgacgtg aaacgccgtc tgcgtgcgcg ccgcgcccgc ctgggcctgc agcgcattca
     1201 cctgcgcctg cgcggcacgc tgcgctgcct cggcctgctc cagggcggcc ttggcgatgt
     1261 agttgcgctc gtagagctgg cgcttgcggg ccagttcggc cgccgccacc tccagtgcgg
     1321 cacgcgcggc caccacctgg gcctgactgg ccgccgcgcc ctgctcggcg gcacgggcgt
     1381 cgatgcgcac cagggtctgt ccggcgcgca cccggtcgcc cgccttcacg ttcaactcca
     1441 gcacggcgcc gggcacctgg gccgccagca ccgactggcg caccgcctcc accgtgcctt
     1501 caaacgcctg ggccgaacgt gcggcctggg cattcaccgt catgtgcggc agctcctgcg
     1561 ccgacaccgg cagcaccagc gcgccgcagg cggccagcag cagcacacct gcagcccgtg
     1621 agccacgcca cgcaaaaagg ggtttgcttg tcaccgtcat tgcgatatta tttgcgcaat
     1681 cacgcaaata tgcaatgttg caaataccca acgaccccgg ggcaccgcta gactgcgggg
     1741 aacacccgtc caacttgcag gtggttttct tggcctcgat gcagcaactt ccccccgaag
     1801 cgcttgagca tgtggcgcat tactttcgca cgctgtccga ggcaacgcgc ctgcgcatcc
     1861 tgaacgtgct ggggcatggc gagatgagcg ttggcgacat cgccgagaag gtggattcca
     1921 gcgtcgccaa cgtgtcgcgc catctggcgc agatggcgca gcacggcctg gtggcacgtg
     1981 aaagccgggg caacagcgcc tactaccgca tcgccgaccc ggccgtgcac cagttgtgcg
     2041 atctggtgtg cggcaacatc gcgcgccgct tcgaggacgt ggccagcacc cgcgccgcct
     2101 tcgtccgcac cgcgcgcgcc aaacccacga ccggccgctg aacggccacc cggtcagctg
     2161 gcgcgaacca ccagggcgtc gatcttggtg tgcgacagca cgctttgcgc cacgctgccc
     2221 agcaccagct tttcgagtcc gcggcggccg tgcgacccca tcacgatcag gtcggcacca
     2281 acggcgccgg ccgcctccag aatgccgcgc cacacggcgt gggactccac cacccggctg
     2341 tcgacctgca ccccagccgc ctgcagacgc tggttggcgg cttcgatcgc cgctcccgcc
     2401 tccgcccgcg ccgcgttcag gtactggtcc tgcccgtagg cgaagtcggc gcccacgccg
     2461 gtgaacgggt agggatcgat cacgaagatc gccgtcaccg gcacgccgta cgcccgcgcc
     2521 agccccgctg ccttgtcgat ggctgccatc gacgcggtcg atccgtcgac cggaaccaga
     2581 atgtgcttgt acatgatgga ctccttggtt gcctatgcgc ctgaaaccat tctctgatcg
     2641 catgcccggc cgcagtttga cacgcatcaa gtatcagggc ccgtcaagcc ccaacctccc
     2701 gtcacggcag cacgctgcgg atgcgccggg gcgcgcccgc ccgggttgac gacaatcggc
     2761 gtgctgcccg gcgcgcggcc acctcatgcc catggacacc atcaaccttg cactcgtggg
     2821 catcagcctg gggctggccc tgtcgtttcg accctggcgg ctgctggcgg gcggcgccct
     2881 gctctcgccc ttgctggcat cgctggtcat cacgccctgg ctgtgggccc tgccctggct
     2941 gcaccggatg ccgatccagt tgcagctgtc cggtgcctgc ctgatcgggc tggccctggg
     3001 ctggccgctg gcggtaccgg tgttctgcct gattgcggtg ctggtcggcc tgatcgcacc
     3061 ggtgagctgg gcacagcagc tggacatggc actgtggctg ggcatcgtgc cggccaccct
     3121 gtcgttcggc ctcggcatgg tgctgcggcg ctgggtctgg cgcaacctgt tcgtctacat
     3181 cctggggcgc gcgtttctgg gcaccgcgct gtgcatgttc gccagcgggg ccctggcgca
     3241 gtggacgggt caccgcctgg ccgccgcggt ggaccccgac ctggccatgg tggcgcgctg
     3301 gttgatcgcc tggggggatg ccttcatgac cggcatgatg gccgcgatct gcgtggcctt
     3361 caggccacat tggctggcca cctggtcaga ctggctgtac atcgcaccgc caccgtcgcc
     3421 ccgatcgaac cagccctgac gttgaggcgg atcaagttcg gccatgtcca taacatggat
     3481 actcgtcacg atccgatgaa ccaaagagga atgggacgat gaaactgtgg caagacctgt
     3541 tcggcaccga ctacggactg atgagcatcg ccggcatcat cttcatgatc ggcatggcga
     3601 tctggttcgt gcgcttcttt ctgcgcaaga tcgcgcagtc gtccgatcag caatgaactg
     3661 accgggcggc ttcagcccct gggcgcctcg gccgcctcca gcagcagcgc ggtctgacgc
     3721 gcgagcaact gcagcgtgcg cagctgcacc ggcgacagcg ggcccgcacg ccggtcggcc
     3781 acccagagca cgccccacac cttgcccgag gcgccgacca gcggagcgcc ggcccaggtg
     3841 cggatggcca ggccctgcgc cagcaaggcg cagccgctga accgcggatc accccccagg
     3901 tcttcgatca ccgtcacctg atcggcgcag cgcatcgcca gttcgcaggg tgagccaggc
     3961 agcggcagcg cctccacggc gaccccggcc tgcgctttga accgaagcca cggtccatcg
     4021 cgcagcgcca tcgcccccag cggcatgcgg cagatgcgca acgccgtctc caccagatag
     4081 gcatgggcgt cgtccccccg cgcctgcagg gcggccaggc gccgctgctc ggccgcgcgc
     4141 ctgggcgccg gcacctgcgc cgacaaggcg gcctgcacgg gggcatcgcc cggccgggag
     4201 gtcaggcgcg cacgcgcctc gggcggggtc ggcaccttgg cggcggccgc cggggcgctc
     4261 aaaaatctgc tgacgaacca cccccacatg cccacaccct gaatgtaact ttttgttgat
     4321 tggtcgcgat tattgcgcat ccgcagccgg tcgacaggtt tttttgcgcg gcccccgcgc
     4381 cgccaccggc ggcgacaatc ggcgccatgg gtttgcggct cttgcgacac atggccatcg
     4441 gtctggccgg catgctggcg ctgggcgcca tggcggcgcc ggccttcgac caggtgcggg
     4501 ccgacttccg gccctccgaa accgtgctgc tggatcgcag cggccagccg cttcatcgcc
     4561 tgcgcaccga cgcgcgcgtg cgccgcggcg attgggtgcg gctggaccag gtctcgccgg
     4621 ccctgcgcca ggcgctggtg ctgtcggagg acaagcgctt ctacgagcac agcggggtcg
     4681 actggcacgc cgtgggcgcc gccgcctggg ccaacctgtg gcacacgcgc acgcgtggcg
     4741 cctcgacctt gaccatgcag ctggccggcc tgctggacga cgacctgcgc ctgggcagcg
     4801 gcggtcgcag cctgcgccag aaggtcgggc aggtggtgct ggccacgcag ctcgaagcgc
     4861 agtggcgcaa ggaccagatc ctggaggcct acctgaacct ggtgccctgg cgtggcgagc
     4921 tggtcggcat cgatgcgctc tcgcgcaccc tgtttgccaa ggcgccgcat gggctgcagg
     4981 acggcgaagc cgccatcgcc gccgccctgg tgcgggcccc caacgccccc gcggcccagg
     5041 tcgcgcggcg cgcctgcggc gtgctgcaga gcatgcgtgg cgaccgcgcc gccgccgatt
     5101 gcgtggcgct ggacatgcag accgaagccg cgctggcgcg ccgcgccttc gcgcccagcg
     5161 acggcatcgc gccgcacttt gcgcgccgcc tgctcgccgc cacccgcaac ggcagcgacc
     5221 gcgcccccgc cacgctgccc agcacgctcg acggccggct gcagcgcctg gcgctggagg
     5281 cgctgcaagg ccagctgcgc gaactgcgcg gccgccatgt ggccgacggc gccgtcatcg
     5341 tgctggacaa cgccagcggc gaggtgctgg cctgggtggg ctccagcggt gcgctgagca
     5401 gcgccgccga ggtcgatggc gtgctcgccc cacgccagcc cggcagtacg ctgaaaccct
     5461 ttttgtacgc gcaggccatc gccgagcggc gcatcacggc cgcgtcgctg ctgcacgatt
     5521 cgcccgcgca actgcacacc gccagcggcc tgtacatccc gcgcaactac gacctgcagt
     5581 tcaagggctg ggtgtcggca cgcaccgcgc tggccgcctc gctcaacgtg ccggcggtgc
     5641 gcgtgggcgt gatggtctcg ccgcgcgcct ttgcgcagca actggggcgg ctgggcattg
     5701 agctggccga aagcggcgac tactacggct acagcctggc cctgggcagc gccgaggtca
     5761 gcctactgca gctcaccaac gcctatcgcg cgctggccaa tggcgggcgc ctgtgtccgg
     5821 tgcggctgct gcccaatgcc cccggatcac tccctctccc tctgggagag ggccggggtg
     5881 agaccgaaac tcactctcgc gccgcgagcc tttcactccc tctcccgtct gtgggagagg
     5941 gtcggggtga gggcaaccgg agcccgcagg tcactgccac cacgccctgc acgcaggccc
     6001 tcgacgccgg cgccgccttc atcaccagcg acatcctgtc cgaccgcaac gcccgcgccc
     6061 ccaccttcgg caccgactcg atcctggcca cgcgcttctg gagcgccgtc aagaccggca
     6121 ccagcaagga catgcgcgac aactgggcgg tcggctacag ccgccgctac accgtcggcg
     6181 tatgggtcgg caacgccagc ggcgccgcca tgtgggacgt cagcggcacc agcggcgccg
     6241 cccccgtctg ggccacgctg atgcgcgagc tgcaccggca caccccctca ccgccccccg
     6301 ccccacccgc tggcctacag cacgtcgccg tgcactacgc cgccggcctg caggcgccgc
     6361 gcagcgagtg gttcctgccc ggcaccgcac agagtgaatt cgctatagaa acagaagctg
     6421 ccagcgcaga cccagcaaag gctggaggcc aaaaaggctt gaaaacggcc agccacccca
     6481 gcgcccccgc cggcgtgccc ccgcgcatca ccgcgccgca agacggcacc atcctcgccc
     6541 tcgaccccga catccccccc gcccatcagc gcctgcgcct ggccgccgaa cccggcagcg
     6601 cccccgccaa cagcctgcgc tggtgggtgg acgatcagcc cattggccgt ggcgcgcagg
     6661 ccgactggct gccgatgccg ggcaggcatc ggatcacctt gtgcgatggg cgcgggcggg
     6721 tggtggatga aaggcggatc gaagtgcggg gcgcccagcc ccgccatccc cgctcagccg
     6781 gggccgccga tcgacttgcg aaacaatagg gcccccacga ggaacaccgt gtttgaacaa
     6841 accttcaaga acatcgacga catcctgcac aaggacgccg gctgcaccag cgaactggac
     6901 tacaccgagc aaagctcctg gctgctgttt ctcaaatacc tggacgacct ggaggccgac
     6961 aaggccgccg aggccgagct ggccggcaag ccctaccgct acatcctcga cgcaccctac
     7021 cgctggtcga gctgggccgc cccgcaagat gcggccggcg cgctggacca caacagcgcc
     7081 ctgaccggcg acgacctgcg cgacttcgtc aaccagcggc tgttcccgta cctggcgggc
     7141 ttcaaggccc gcgccagcgg ccccaacacc atcgaataca agattggcga ggtctttggc
     7201 gagatcaaga acaagctgca aagcggctac accctgcgcg acgtgatcga gcaggtggac
     7261 gcgctgcgct ttcgctcgca gaccgagaag cacgagctct cgcatctgta cgaagccaag
     7321 atccgcaaca tgggcaacgc cggtcgcaac ggcggcgagt actacacccc ccgcccgctg
     7381 atccgcgcca tgatccaagt gaccaagccc cgaatcggcg agcgcatcta cgacggcgcc
     7441 tgcggatcgg ccggctttct gtgcgaggcg ttcgattacc tcagaagcca gccgggcctg
     7501 accaccgccg acctgcagac gctgcagcag cgcagcttct acggcaagga gaaaaagagc
     7561 ctggcctacg tgatcgcgat catgaacatg atcctgcacg gcatcgaggc gcccaacatc
     7621 atccacacca acaccctggc cgagaacctg gccgacatcc aggaccagga ccgctacgac
     7681 atcgtgctcg ccaacccgcc cttcggcggc aaggagcgca aggaggtgca gcagaacttc
     7741 ccgatcaaga ccggggaaac cgccttcctg tttttgcagc acttcatcaa gagcctcaag
     7801 gccggcggcc gcgccgccat cgtcatcaag aacaccttcc tctccaacac cgacaacgcc
     7861 tcggtcagcc tgcgcaagct gctgctggag ggctgcaacc tgcacaccgt gctcgactgc
     7921 cccggcggca ccttccaggg cgcgggcgtc aagaccgtgg tgctgttctt tgaaaaaggc
     7981 gcgcccacgc gcagcatctg gtactaccag ctcgacccgg gccgcagcct gggcaagacc
     8041 aacccgctca acgacgccga cctggccgag ttcgtgacgc tacaggccag ctttgccgat
     8101 tcagcgcaaa gctggtcggt ggatgccagc cgcatagacg ccgccagctg cgacctgtcg
     8161 gtcaggaacc ccaatggcgg cgaggccgtg gcgctgcgca gcccgcacga aatcctggac
     8221 gagatcgcgg cactcgatgc cgagagtgcc gaggcgctgg cgaggattcg ggagttggtg
     8281 gcatgaatgg gagatggcca gttcgaccct tgggctcact atgccgcatt gcacgaggag
     8341 gctcgccgcg cccgattcaa gcgttcttga ccgatactga tgacgggatc aattggatca
     8401 agatcagcga tgcgacggcg agtggaaagt acatttattc cacagttgag aagatcaaac
     8461 cttcaggtgt gtcgagatca cgcatggtga aggaagggga tttcctactt tcgaattcca
     8521 tgagctttgg ccgcccatac atcatgcgga cgatggggtg catccatgac ggctggctcg
     8581 ttctttctgg gtaccagaat ttcttcgatc aagatttttt gtaccactta ctcggatcac
     8641 cacaagtctt ccaacagttc aatcaacttg cagctggatc gactgtcaga aacctgaaca
     8701 ttgatctggc cagccgcgtt cagcttccta ttccatcgct ccccgaacag cgccgcatcg
     8761 tcgccatcct tgacgaagcc ttcgacgcca tcgccaccgc caaggccaat actgaggcga
     8821 attggcaggg tgcaagggcg gtgctcaacc aatgtcggga tgacgcgctg tcctccttgg
     8881 cggccgagtt cggcaccgtg aatctcggtt cagtttgcga ttttgagaat ggagacagag
     8941 gcaaaaacta ccctggaaaa caacaccgtg tggctgaagg agtgcctttt atcaacgctg
     9001 gtcatctcac cgaagatgga atcgactttg aatcaatgga ctacatctct cctgagcgtt
     9061 attcgctgct agggaatggc aagattaagc caagagatat cctgttttgc ttgcgaggtt
     9121 ctctggggaa atttgctaac gttggcgata cccccgttgg ggcaattgca tcgtcactag
     9181 tcattttgag accgagaagt ccgctctacg acaagtattt agaaatctat cttgcgagca
     9241 gttgttgcat tgaaatgatc aacaaataca aaggcggcgc ggcacaaccg aacttgggtg
     9301 ccaaagactt aaagcagttc gtgcttccgc ttcctccaca gagcctgcag cggcaactaa
     9361 ctgagcgact tgacggaatt aaagaacaga cccatctcct agaggcaatc tactcagaga
     9421 aatctgccgc cctcgacgaa ctcaagaaat ccctgctgca cctggccttc accggccaac
     9481 tgaccgcccg ggccgccgac cggcaactgc aggccgtggc atgaacgagg ccgaaacccg
     9541 cgccgagcac atcgaccctg ccctggcggc ggcgggctgg ggcgtggtgg agggcagccg
     9601 catccgccgc gaggtgatcg cccccggccg cctgcagggg cgcggccagc gcgcgcgggc
     9661 cgacattgcc gactatgtgc tgatctaccg caacaccaag ctggcggtgg tcgaggccaa
     9721 ggcctgggac aagccccaca ccgaggggct ggcgcaggcc aagcgctacg cggccaagct
     9781 ggcgctgcgc tttgcctatg ccaccaatgg ccagcagatc tacggcgtgg acatggccac
     9841 aggtgccgag ggcgacgtgc cggccttccc cggcccggat gcgctgtggg cgcagacctt
     9901 tgcccaggcc aatgcctggc gcgaccgctt tgccgccgtg ccgttcgagg acaagggcgg
     9961 cgcgtgggaa agccgctatt accaggagat cgcgatccgc cgcgtgctgg aggccatcgc
    10021 cggcgggcaa agccgcctcc tgctgacgct ggccaccggc acgggcaaga cggcgattgc
    10081 ctttcagatc gcgtggaagc tctaccacag ccgctggaac ctgggcgact ggcaggcgcc
    10141 gggcgcgccc acgcggcgcc cgcgcatttt gtttctggcc gaccgcaaca tcctggccga
    10201 ccaggcctac aacgccttca gcgcctttgc cgacgatgcg ctggtgcgca tcgcgccggg
    10261 cgaaatccgc aagaagggcc gcgtgcccag gaacggcagc gtcttcttca ccatcttcca
    10321 gaccttcatg agcgggccgg gcggcgcgcc gcatttcggc gcgtacccgc cggacttctt
    10381 cgacttcatc gtcatcgacg aatgccaccg cggcggcgcc agcgacgaga gccagtggcg
    10441 cgccatcctg gagcactttg ccccggcggt gcagctgggc ctgacggcca cgcccaggcg
    10501 gcgcagcaac gcagacacct acgcctactt cggcgagccg gtctacacct attcgctgaa
    10561 agagggcatc aacgacggtt acctcacgcc cttcaaggtg cggcagttcg ccaccacgct
    10621 ggacgactac acctacaccc ccgacgacgc cgtggtggaa ggcgaggtgg tgcccggcaa
    10681 gcgctatgtc gagggcgagt tcaaccgcgt gatcgtgatc cgcgagcgcg agcaataccg
    10741 cgtggggctc ttcatgggca tgatcgatcc gcgcgaaaag acgctggtgt tctgcgccac
    10801 gcaggagcac gcgctgatgg tgcgcgacct gatcaaccag gccaagacca gccccgaccc
    10861 gaactactgc atgcgcgtga cggcggacga tggggcgatg ggcgagcagt tcctgcgcga
    10921 ctttcaggac aacgagaagt ccatccccac catcctcacc acctcgcaga agctctcgac
    10981 cggcgtcgat gcgcgcaacg tgcgcaacat cgtgctgatg cggccggtgg cgtcgatgat
    11041 cgagttcaag cagatcatcg ggcgcggcac gcgcctgttc gacggcaagg actacttcac
    11101 gatctacgac ttcgtcaagg cgcaccacca tttcagcgac ccggagtggg acggcgagcc
    11161 ggtggagccg gagccgccgc gcccgcgtgc gccggcgccg accgccgacc cgggcgaacc
    11221 gcctgccgtg accgaagtgc cgcccgcgta caggatcagg atcaaactgg ccgacggcaa
    11281 ggcgcgcgcg atccagagca tgacggccac cagcttctgg ggcgcggacg gccggccgct
    11341 gtcggccgcg cagttccttg aaatgctgtt cggcgcgctg cccgagttct tcaaggacga
    11401 ggacgaattg cgcgccctct ggagcgaccc ggcaacgcgc agaaggctgc tggaccagct
    11461 ggccgagcgc ggcttcggcc gcgagccgct ggccgagatg cagaagatca tcgaggccga
    11521 gcacagcgac ctgttcgacg tgctggccta tgtggccttt gccacccagc ccaggacccg
    11581 cgcccagcgc gccgacgccg ccaaggccgc cgccgcgccc ggcctgagcg acaagcagca
    11641 ggccttcgtc gatttcgtgc tggcgcagta cgtccagcag ggcgtggacg agctcgatca
    11701 ggacaagctc tcgcccctgc tgcgcctgaa gtaccgcgcg ctgcccgacg cctttgccga
    11761 actcggccgg cccgagcaga ttcgcggcgt ctttgtggac ctgcaacggc atctgtacct
    11821 ggattgagag cggccccggc tgccgcgtgc caggccgggc ggcaccgcgc aaggcgcagc
    11881 gctggcgttc ttccactggc catcccgcgt gccgcgcagg gcgcttgtcc gagtcccatt
    11941 ttgggactaa actgcaaacc gtgcgagtga ttgccgtgtc tacccttcgg gccttctggc
    12001 agcgccaccc cgacgccgag cagccgttga acgcctggta cgaggaagcc acgagcgcga
    12061 cctggaccca gcccgccgac atcaaggcgc gctaccgcag cgccagtgtg ctgaagaacc
    12121 gtcgtgtggt cttcaacatc aagggcaatg actaccggct gatcgtggcc attgcgtaca
    12181 ggttgcagat cgtctacgtg aaatttgtcg gcacccatca ggagtacgac gccgttgatg
    12241 cagaaaccat tgaatcggcc tgagcggcca cggagaaccc catggacatt cgccccatcc
    12301 gcaccgaagc agactaccag gccactctca aggagatttc gggcttgatg gcttgcgacc
    12361 ccgagctggg tacgccagag ggtgatcgcc tcgacattct ggccacgctg gtacaagcct
    12421 atgaagccaa gcactttgcc atcggcgcgc ccgacccggt ggaggccatc aaattccgga
    12481 tggagcaaag cggtctgtcc gtcaaggacc tggagccgct catcggcaag agcaaccgcg
    12541 tctacgaagt cctgaaccgc aaacgcccgc tgacgctggc catgatccgc cggttgcacc
    12601 aaagcctggg catcccggcc gatgtgctga ttgcggagac cgcggccggg tgatcggcgg
    12661 cctgggtaac ggggtcagat gggaaacggg gggaaacggg gtcagatgaa aattttctcc
    12721 caagccgggc cagtgtcccc accaggccgg ccgacgcagt cttcgaccgg ctccaggcca
    12781 agtacgccgg caagtccaag cgctgatgca actggccttc acgccgctgg tcgagcacaa
    12841 gcttgaggcg attgccgact gaatcccggc caacatccgc cgggccacac gggttgcagc
    12901 aacctttatt cggcaccgcc gggtttcacc cgcccgccac cgccggacac tcctgccgca
    12961 gcagccgcgc cagctcgcgc ttgcgctcga tcggcaggcg cggcgtgatg gcgccaaaac
    13021 tcagcgccgc gatggctccg ggcgcggcgg caaaggtgac gcccacgccc gacacgccgg
    13081 cggtcagggt gtcggtgatg tcggcccagc cctgggcgcg ggtctggtgc acggcgcgca
    13141 gcaggcgctc gcgcgtgaag tcggcctgcg cgtattccgc cgcgttgcgc tcgtagatgc
    13201 gcagcacctc cgcatcgggc aggcgcgcca tgatggccag gccgcccgcg cccaggccca
    13261 gcaggcgccg cccgccaatg tcggtggtaa acaccttgac cggaaacggc ccctcctcgc
    13321 ggtgcaggca caggcagtgg tcgccctggc ggatgaccag aaacaccgtg tcgcccgaca
    13381 tgcgcgccag ccgctgcatc agcgcgcggc actggtcgac cagcggcacg cgccgcatgg
    13441 cggcaaagcc cagctgcatg gcgtccaccc ccagccgata gaccttgcca cccgcgtcgc
    13501 gctcggcgaa ctgctcctcg accaggcagg ccagcaggcg gtggcaggtg gagcgctgca
    13561 ggcccgaggc ggccatggcc tcgctcaggc ggatgccgtc ttcctgatgc tcggccagca
    13621 ggcgcagcag ttgcagccca cggcgcaggc tttgagcccc tgcggtcgat ctgtccatga
    13681 tgtgggaatt ttttaataaa ttgtttgcag tgcaggaatg gtacttccta gactgcggcc
    13741 aagccacccc acccctaccc gagattgaca cccatggcat ttgacaccct ggcgatcgaa
    13801 acccacggcg cggtgcgccg catcctgctg gcgcgaccgg cgcagcgcaa cgcgcaaaac
    13861 tggcagctgc tcgacgagct gatggccgcg ctggatcagg cgcgcaccga cgacgccatc
    13921 cacgccgtgg tgctgggcgg gcaaggcgag catttctcgg ccgggcacga cctcaagcag
    13981 gcgcaggccg agcgcgccaa cttcacggtc gagcagcgct gggcgttcga ggagctgcgc
    14041 tatttcgact actcgatgaa gatctgggac tttcccaagc ccaccatcgc ccaggtgcag
    14101 ggcgcctgcg tggccggcgg cttcatgatc gccaacatgt gcgacctggt ggtggccggc
    14161 gagagcgcgt atttttccga cccggtgggc cacaccctgg gcgcggcggc caccgaggtg
    14221 ctgatccacc cctgggtgat gggcgcgcgc aaggccaagg agctgctgtt caccggcgcg
    14281 cgcatgagcg cgcaggaggc gcaggccatc ggcatggtca accgtgtggt gcccgacgcc
    14341 gagctggccg acgccacgct ggcgctggcg cagcagatcg ccaaggcacc gccctttggc
    14401 ctgcgcctga tcaagcgctc gatcaaccgc acgctggacg cgcagggcct gcgcagcgcg
    14461 ctggcggcgc acttcgacac gcaccagctg tcgcacctga gcgatggctt tctgagcgcg
    14521 cgcgaccgcg gcctggcaag cgccatccag aagaacccgg ccaaggcgca gcagtgaccg
    14581 gcatgcatcg tctgcaaccc ctgctcgacc cggcctcgat cgccatcgtc ggcgccagca
    14641 acaacccggc gcgcatcggc ggcatgccga tcgcccattt gacgaagttc ggctacgccg
    14701 gcgcgatcta cccgatcaac cccaagtacc aggaggtgtt tggcctgcgc tgctggcccg
    14761 acatcgaagc gctgcccgcc gcgcccgatc tggtggtgct ggccattgcc gcgcccgagg
    14821 tgctgcccat gctgcgccgc tgcgcggcca agggcgtgcg cgccgtcatc gtctatgcgg
    14881 cgggctttgc cgaagaaggc gagcgcggcg cggcgctgca ggccgagctg gccgcctacg
    14941 tggccagcac cgacatggtg gtggccggcc ccaacgccat gggctttgcc aacctcaaca
    15001 cccaggcgca caccaacttt gcctcggtat tcaacaccgc gcccatgcag gcgggcacgg
    15061 gcagcgtcag cctgctgacg caaagcggca acgtgtgcgc ggcggtgtac gccatcgcgc
    15121 gccggctggg cgtggccttc agtcacttca tcaacaccgg caacgaagcc tgcgtggact
    15181 ttgcccagta cctggagtac ctggcgcagg acgaggccac cgacgtctgc atcggctaca
    15241 tcgaggagct gcgcgacggc gcgcgtttca tcgccgccgc cgaggaattt gcgcggcgcg
    15301 acaagctgct catcctgtac aaggcgggcg agaccgacaa gggcagcgag gcggtgcgct
    15361 cgcacacctc ggcgctggcg ggcgaccagg ccatttaccg cgctgccttt cgtcagctcg
    15421 acatcatcca gagccacgac ttcgtgcaga tggcgcagct ggcgcatctg gccagctacc
    15481 gccggcgccg cgccggcaag cgggtggcca tcatcaccat cagcggggcg ctgggcgcca
    15541 tcctggccga caagttcatc ggcgccgggc tggaagtgcc caccctgccc gccgagctgc
    15601 agcagcagct gcgcgccggc atcccggact acggcatggt cgccaacccg gtggacgtga
    15661 ccggcaacgt ggtcaacgac ccgggcttcg tgcgcaccgc gctggaagcg ctggccacca
    15721 gcgacgcggt ggacacggtg gtgatctacg cccccggcta catgctcgac cgcatggccg
    15781 gcgacctgtg cgccgtcgcg gccgcgcacc ccaggctgtt cgtggccatc gacaccggcg
    15841 ccgcccagag ccgcgagcag ctgcgcgccg gcggcgtgcc ggtgttcgag gacatcggcg
    15901 tggccaccgc cgcgctggcg ccctttctgc tgtggcagca gcagcgccag cgcaaccgct
    15961 ggcgggcgct gcgccaccag gcgccaccgc ccgccaccgc gctgcccgag gtgctggatg
    16021 agcaccagtg caaggccgtg ctggccgcca gcggcgtgcc cgccgcgccc gagcgccgcg
    16081 cgcgcagcgc cgacgaggcg gtggccgcgg ccgacgccct gggctacccg gtggcgctca
    16141 aggtgctgtc gcccgacatc gcccacaaga ccgaagccgg cggcgtgcgc ctgcacctgg
    16201 ccgatggcgc gcaggtgcgc gccgccttcg accagatcct ggccagcgcg cgcgcccacg
    16261 cgccgcaggc gcgcctcgac ggcgtgctgg tggcgcccat gcagtctggc gtcgccgagc
    16321 tgatcgtcgg cgccacgcgc gacccggtgt ttggcatggc gctcaccgtc ggcctgggcg
    16381 gcgtgctgac cgagctgtac caggacgtca gccaccgcct gctgccggtg gacgaggcca
    16441 tggccgccga catgctggcc gagctcaagg cctatccgct gctgaccggc taccgcggcc
    16501 gcccgccggc cgatgtcgat gcggcctgtg ccgccattgc cgcgttctcg cgcgccgtgc
    16561 aggcgctggg ccggcaggtg agcgaagccg aggtcaaccc gctgctcatc aaagccagcg
    16621 gccagggcgc cgccatgctg gacgcgctgc tgctggcccc cgccgattga atttcaaccc
    16681 accccaaccc caaggagact caccatgatc aagcaactgc tggccggcgt ggcgctggcc
    16741 gctgcggccc tgtgcacccc ggcacaggcc cagggctacc ccagcaaacc ggtcaacatc
    16801 gtcgtgccgt tcgcccccgg tggcgccacc gacatcatga cgcgcctgct ggccgaacgc
    16861 ctgagcaagc gcctgggcca gcccgtgatc gtcgagaaca agcccggcgc cggcaccatg
    16921 atcgcgtccg agtacgtggc caaggcgccg gccgatggcc acaccgtgct gatggcggcg
    16981 tcgtcgctgg gcatcgcgcc ctcgctctac agcaaggtca attacgaccc ggtcaagagc
    17041 ttcgcgccga tctcgctggt ggcctcggtg gtgcacgtgc tggaagtgca ccccagcgtg
    17101 ccggtcaaga gcgtcggcga gctgatcacc tggctcaagg ccaatccggg caaggccaac
    17161 tacggctcgg tcggcgccgg cacctccacc cacctggaat ccgagctgtt caacaccatg
    17221 gccggtgtga agatggtgca cgtcccttac aaaggcagcg ccccggcgct gacggacctg
    17281 gtgggtggca gcctgcaggt gatgttcgac gcctgggcct cgtccggccc cttcgtcaag
    17341 gacggcagaa cgcgcctgct ggccgtgacc acggcgcagc gctccaagat cctgcccgac
    17401 gtgcccaccg tggccgagtc cggcctgccc ggctacgaag ccatgccctg gctgggcctg
    17461 ctggccccgg ccggcacgcc cgacgccgtg gtcaagaagt tccacgacga gctgatggaa
    17521 gtgctcaagg agcaggcggt gcaggacaag ttccacagcc tgggcctgga catcatcggc
    17581 aacacgccgc aggagtttgc cgacttcatc aagaaggaca tcaccaagtg ggccaaggtg
    17641 atccaggaaa ccggcgccaa ggccaactga agccagctcc acgaatgcaa tcattttgat
    17701 agctgttagc gcctgcccag cgggcgctgc ggcttgaaag caaaggaact ctgatgcagg
    17761 tcggctacgc cgccgaggac gaagcctttc gcgccgagct gcgcgcgtgg ttcgagaccc
    17821 actacccgcg cttcgtcgcc gcctggcccc aggcgcccga ccccaacgcc ctggactggc
    17881 gtcgcgcctg ggaagactac gtctgccagg ccggctggtc cggcctgggc tggccgctgg
    17941 cctacggcgg caaggcctgg tcgctcacgc gccaggccat ctttcacgag gagcaggcac
    18001 gcatcggcgc gccgctgggc gtcaatctga tcggccacgg cattctggcg cccacgctaa
    18061 tccacttcgc cagcgaggcg caaaagcacc gctacctgcc cggcatcctg tccaaccgcg
    18121 aggtctggtg ccagggctat tccgaaccgg gcgcgggctc cgatctggcg gcgcttggta
    18181 cgcgcgccga gcgccgcggc gaccactacc tgctgaacgg ccacaagatc tggacctcgt
    18241 tcgcgcacat cgccgactac tgcttcgtgc tggcgcgcac cgaccccgca gcccagcgcc
    18301 acaagggcat cagctttctg ctggtggaca tgaaaagccc cggcgtgcgc gtgcagccga
    18361 tccgccagat caccggcgag gccgatttca acgaagtctt ctttgaagac gtgcgcgtgc
    18421 cgctggacgc gctggtgggt gaggaaaacg gcggctggcg cctggccatg gcggccgcca
    18481 gcttcgagcg tggcacctat ttcattccgc gtcaggtgcg ctttgcgcaa gaggtcaagg
    18541 cgctgcagcg cctggccatc gacaccggcg ccgatcgcga cgcgcacctg cgccgcgagg
    18601 tcacgcgcct ggcgctggac agccacctgc tgcgcctgaa gggcttgcgc gcgctcacgc
    18661 tggcgctgcg cggcggcccg cccggcgccg agggctcggc caccaagctg cactggagcg
    18721 aggcgcacca gcgcctgacc agcaccgcgg ccgagctgct gggcgagcgc gccctcgctg
    18781 gccccgcgcc cggcgggcac gacccggcgg ccgccctgtg ggcgcgcgaa tttttgtgga
    18841 cgcgcgccga aaccattctg gccggcacct ccgaagtgca acgcaatatc ctggccgagc
    18901 agatgctggg cctgcccagg taagcccatg gactttgccc tgaaccaagc gcagcagatg
    18961 ctgcaggcgt cggcgcgccg cttttttgcc gaccaccacc ccctgacccg cgcgcgccag
    19021 gccctgccct ggagcgacgc cgcccagcag cgcctgtggg ccgacatggc cgacatgggc
    19081 tttctggccc tgctgctgcc cgaggaccag ggcggcctgg ccctgggcct gaccgaagcc
    19141 agcctgctgg ccgaggaagc cggccgccag ctgctcaacc tgccctgggc cgccagtgcc
    19201 gtctggctgg cgctggcctg ccgcgccgcg cccgccagcg cgcccgcgcc cctgcgcgat
    19261 gccgtgcagg ccatgctggc cggccggcgc gccctgcacg tggtcgatgc gggcgatggc
    19321 tggtgggacc acgccggtca gtgcagcgac atcgtcgtga ttcgcgaaag ccccgcgccg
    19381 ggcgagccgc tgcagctggc gctgatcgat ggcgccgccg tgagcgccgc gcccgcgctc
    19441 gaccccacgc tgcgccaggc tccggcgctg gcgcctgccg ggccctggca cgactgggcg
    19501 ctggacgctg ccacccgcgc ccaggcgcgc accggctacc ggctgatgcg cgcggccgag
    19561 ctggtcggcg tggctcaggc tgcgctcgaa ctggccgccg gctacgccag ggagcgcgtg
    19621 cagtttggcc gcccgatcgg cagcaaccag gccatcaagc accagctggc caacgcctgg
    19681 atgggcgtgg acaacgcgcg cctggccgtg ctgtacgcca ccgccgcgct gaacgagcgc
    19741 ctgcccgacg cccgcttggc ctgcgccgcc gccgaggcga gcgccatcga gggcgcgctg
    19801 caggccaccc gcagcgccct gcaggtgcac ggcggcatgg gcttcacctg ggagagcgac
    19861 gtgcacctgt acctcaagcg cgcccagcac ctggccaccc gcctgggcgg cgccagccgc
    19921 gcgttgcagc agctggaggc gcaggtctgg gatgccgcgg cgaatgcgcc atctgcgaca
    19981 ccggtttgaa ggcgtggacc cgtgtcgcgc cgccgatggt cgaaaacccg acctcggcgc
    20041 tgacatgact acagcatcgc actcgaattg ccattgtttc gatagcgttc ggcgcagtcc
    20101 agaaaagcgc cagaagccaa tttcaatcgg attgtcggtc accgcccggc accacaccgg
    20161 ccccttgcgg tggtaggccg cgctccgacc caaagccccc gcgtccgcgc tatcgtgcct
    20221 cgcccgccag cgccagctgc cgtgacgaag cctgtcgcgc ggcctgcacc accagttccg
    20281 ccaggcgctt gacgccacgc gcccggatgc gctctgccgg gcctgagatg ccgagcgccg
    20341 ccacgacgcg ctggcgctca tcgaacaccg gcgccgcaat gccccagacg ctggcgcgcc
    20401 attcgcctcg gttgacggcg taaccctgct gccgcacgcg atcgagctcc atcgccagcg
    20461 ccgaagtgtc ggccagcgtg gtcggcgtgt agacctgcag gggccgcacg gtcatcagct
    20521 gaaccgccgg ctgttcgcgc caggccagca gcgccttgcc cgtggccacg caataggccg
    20581 gtgcgcggcc cccgatgacg ctgtaggcgc gcaccggctc agggctgtcg agcttgtcga
    20641 tgtagatcac ctcgtcgcca ctgagcaccg acagatgcac cgtttcgttg gtttccgcca
    20701 gcagcatgcg catggccggc gctgcggttt cacggaaatc cagtttgtgc agcgcctgcg
    20761 cccccagctc ccacaccttg agcgaggccc gataaccacc acgcccgccc tcgttgacgg
    20821 catagccgag ctcgaccagc gcctgcagca agcggtgcac gttgctggcg cccatctcca
    20881 gctcctgggc cagctcgctg attcccacgg gctgtgcgcg tgcaaccagc acctcgagca
    20941 agcgcagccc cttgatcaga gtgttgttca ttcgatcacc gccctcaatt tctatatgtc
    21001 ggaacaatta tctcatttac agaatttttc aacacagctt ccgaatcggc aacgggcacc
    21061 cgcgatccgc atgttgtggc cgtcaccgct cccgcatggc cacgccaacc cgtgccatcg
    21121 cccctgacct gcgcacattg gccgcgggag tgcgccttcc agccccctgc gcggcagaca
    21181 catggcaccg gtcacggcat caacgggccc gggaccgcgc tgaacgctgg cgctcacggc
    21241 ccggtgcagc ccgataggtc ttggggctgc atcccatttc gcgcgtgaac atgtaggaaa
    21301 acgcggacgg tgaaccgtag cccagttcga gcgccgtcgc cgtcacgctc atgccggcgc
    21361 ccagcagttc catcgacttg aacaggcgca ggcgccgctt ccattcgcga aagttcatgc
    21421 ccagttcggc ctggaaacgg cgcgtcagcg tgcgctccga cgcacccagg cgcgcgcccc
    21481 atgtggcgat gtcatcggcg tcggccggcg cacgatcgat cgcctcgcac agcttgcgca
    21541 acatgggaga ctgcggccag ggcaacggcg cctgaacgcg ttccagccgc ggcagttgat
    21601 cgaggatcag accgtgcacc cgttgcgtat aggccagtgt ggcgacgccc gccacacgct
    21661 gctcgcccag ccacgccgcc tcacggatca actcgcgcag caaggcagac acctgcagca
    21721 acgcatagcg cgccgccatg ccccgaccca cggaagtgcg caaatacaga ctgcgaaatt
    21781 gcgcgccgta gcgcgacgcg accgcgtgca cggtaccggt cggaatccac accgcctgct
    21841 cgggcccgca gaccacgcgc tgatgggcgg cctcgacgac cagtgatccg ctgatggcat
    21901 agatgagttg atgccatgga tgcgagtgag acgggaatcg gtagcccggc gggatgtcct
    21961 gcgcgcgcac gaacaccggc ccgggcaagg tctcgatttc cggcaccttc agcgtcttcc
    22021 acagcatcgc aagccttgag caaccccttg tcgcttcatc gacatattct gtctctttat
    22081 cgaaagcgca caagccggcc gctgcctaca gtctccacat gcgagacaca gaagcttggt
    22141 cgacaacgcc tcgcgcgatg cctgcggata tcgaaccgcc gcgccgcatc gggtttgcgg
    22201 ccactgcgct gttcatttac ctgatcctgc tggccggcta tgtgctgtcc tacgtgcacc
    22261 gcacggcgcc ggcggcgatc gcaggcgagc tgacccaggc cttccaggtc agcggcgcgc
    22321 tgctcggcac gctcgccgcg acctacttct acgtctacac cttattgcag gtgccggtgg
    22381 gggtgctcgc cgataccgtc ggcccacgcg ttgtggtcac tgccggcgca gtggtggccg
    22441 ccgtcggctc gatcctgttc gggctggcgc ccggctggga actggccgcc gtcggccgct
    22501 tgctggtggg catgggcgtg gcggtgacct tcgtggccct gctcaaggtg tgtgccaact
    22561 ggtttccacc acaacgcttt gcaaccttca acggcatcac cctgcttgcc ggcaacctgg
    22621 gcgccgcggc cgccggcgca cccctggcct ggctggtgac ggtggcgtcc tggcgatcgg
    22681 tgttcgtcgg cctgggcctg gcctcgctcg cgctggccgt gctgacatgg atcgtggtgc
    22741 gcgacagtcc ccgcgagtgc ggattcgagg tgtcttacgc cccgccgccc gcggtcgcaa
    22801 cgcactgggc acaggcgctg cgcgccgtgc tggccaatcc ggccagctgg ccaggcttct
    22861 tcgtcaacgt tggggtggcc ggcagcttct tcgcctttgc cggcctgtgg gtggtgccct
    22921 acctgcgcga aggccgcggc ctgtcgcagg cggtggccag ccatcacgcc agcctgctgg
    22981 tgctcggcgt ggcggccggc tcgctcgtgg ttggtctgct gtccgatcgt ctgcgcagcc
    23041 gcatcggcgt gatgcgcttg ttcgccctga tgtacgcgct ctcgtggctg cccctggtgc
    23101 tgcgatggga accgtcgctg gcgctcagct acgcctggtt cttcgtcatg ggcctgttcg
    23161 tgccggcctt cgtgttgacg tggactgtag cgaaggaaat gaacccgcct gaacacgcag
    23221 gcatggccgt ctcgctcgtc aacgtcggca ttttcctggg ggccgggatc ctgcagccgc
    23281 tggccggtgc tctgctcgac gcggtcaggc ctgtgctggg gccgacggcg gcctgggacc
    23341 gagcgatctg gttgttcgca gccagcgccg ctgccggcgc cttgtgcacc ctgttcgtac
    23401 gacacgagta tccagcccct gcgccagcgc attcacgccg tcagaccggg gcggcagcaa
    23461 cggcggctcg acccggcgca ggccacggat cagtgtgtca ctcaaaaaga gggaatcctt
    23521 aattccctta tttggaatca tagtgtcatt ttacggaatc acttaggatg ccacgcctgc
    23581 cgccgatcca agccatccct ggaccatgat gacccccgtc acgcccccca cagccatgcc
    23641 cttgacggct tcccactggg gggtctatga agccacgggc cagggcgagg cgttgcgact
    23701 gcagggattt gcacgcgacc cctcgccctc gccgatcggc ctgtcgatgc tcgacgccgc
    23761 gcgcggtccg gtgcgcgtgc gtcgacctgc ggtacgtcgc agctggtggc aggccatgtg
    23821 cggcgatgcg ccgcacgcgg caccacagct gcgcggcacc gacccgttcg tcgaggtggg
    23881 ctggaacgag gcgatcgacc tggtggcgcg tgagctcgat cgcgtgcgcc tgcagcatgg
    23941 caacgaagcg atcttcggtg gctcctacgg ctggtccagc gcagggcgct ttcaccacgc
    24001 gcagagtcag gtgcatcggt ttctcaacgc catcggcggc tacgtccgac acgccgacac
    24061 ctacagcctg ggtgcggccc gggtgctgct gccgcacatc gtggcaccga tggaagagct
    24121 gttcacccag cacaccagct ggcgcgtcat ggcgcggcac accaggctgt tcatcacctt
    24181 tggtggcgtg ccggccaaga acgcgcaggt cagttcgggc ggggccaccg agcacgcggt
    24241 ccccaccggg ctgcgccgca tggccgctgc gggggtgcgc ttcatcaaca tcagtccggt
    24301 gcgcgacgac atcgacaccg gcggcgaggt ggagtggatc cccattcgcc cgggcaccga
    24361 caccgccctg ctgctggcca tggcgcacac gctgtggacg gaaaaacgtc acgacgccgg
    24421 cttcctggcc agccactgcg tcggctttga acgcgttgcc gcctacctgc ggggcgacac
    24481 cgacggacaa cccaagagcg ccgactgggc ggcccggatc accggcgtgc cggccgagcg
    24541 catcgtccgc ctggcacggg aagcggccgc cacgcgcagc atgctcaaca tcgcctggtc
    24601 gctgcaacgc tcccaccacg gcgagcagcc ttactgggcc ctgatcaccg tggccgccat
    24661 gctgggccag atcgggctgc ccggcggcgg cttcggtgtg ggctatggca gcaccaacat
    24721 gatgggcaac gacaacgcca agttctccgg cccgacgctg ccgcagggac acaacgcggt
    24781 ccgcgccttc attccggtgg cgcgcatcac cgacatgctg gagcagcccg gtgccagctt
    24841 cgactacaac ggccagcgcc acacctaccc cgacattcgc ctgatctact gggccggcgg
    24901 caatccgttc caccaccatc aggacctcaa tcgcctgctc gccgcctggc agtccaagcc
    24961 ggaaaccatc gtcgtccacg agcagtactg gacccccacc gcgaagctgg ccgacatcgt
    25021 gctgccggcc accacggcga tggagcgcga agacatcggc tacgccacgc gcgagcgcta
    25081 catggtggca atgaagcggc tcatcgcgcc ggtgggcgag gcgcgcgacg actacgccat
    25141 cttcagcgat ctggccgaac gcctgggcgt gcgcgaggcg ttcacggagg gacgcgacgc
    25201 ccgcgactgg ctgagcctga tgtacgaaca gtcgcggcag cgcgcggctc aggccggcat
    25261 cgagctgccc ccgttcgacg acttctggac ccggggcctg cttgacctgg cacagcccga
    25321 ggccgaagtc gtgatgctcg accgcttccg cgccgacccg cagagccacc cgctggccac
    25381 gcccagcggc cgcatcgaac tgttcagcgc gcgcatcgcc agcttcggct acgacgactg
    25441 tcccggacac gcctgctggc tggaaccgct ggaatggctc ggcagcccag ccgcagtccg
    25501 gtttccgctg cacctgatca gcgatcaacc ccacaccaag ctgcacagcc agctcgacca
    25561 cgccgcctgc agccaggcca acaaagtcga cggccgtgag ccgctgaccc tgtgccgcac
    25621 cgacgccgag gcccgcggac tcaaggccgg tgacctggtg cgcgtgttca atgcccgcgg
    25681 cgcctgcctg gcggcactgc gcatctccga cgcgctgcgt cccggcgtcg ctcgcatgtc
    25741 gaccggggca tggttcgacc cgacaagctg gctggccagc ggccccggca atgtcgaacg
    25801 acacggcaac cccaacgtgc tgacgccgga catcggcgct tcgcgactgt cccagggctg
    25861 catggcccag acctgtctgg tcgaagtgga gcgctgcacg tccccggaga ccaggccaac
    25921 gccttacctg ctgcctgaat tcgtcggcac cgcacaccct catgagccgg cctgcacctg
    25981 aaacgctgga actcagggca ccaccacctc acccatcgaa acagacgaga tgaatacccc
    26041 agtttgcgac tacctgatcg tcggcggcgg caccgccggc tgcgtgctcg ccaaccggct
    26101 gtcggccgac ccgtcgtgtc gcgtgatcct gctggaagcc ggtggcccgg acgacgacaa
    26161 atggatccac atcccggccg gcatccgcta cctgctgcgg gaaaaaaagc acaactggtt
    26221 ctacatgacc gagcccagcc cgctgaccaa cggccggtcg gtgtactggc cgcgcggcaa
    26281 agtgctgggc ggctcgagca gcatcaacgg catggtctac atccgtggcc agcgcacgga
    26341 cttcgaccgc tgggccgcat cgggcgccac cggctgggac tgggacagcc tgtttccgta
    26401 cttcctgaag atcgaaaacc agacccgggg cgccgacgcc taccacgcgg tcggcggccc
    26461 gctgacggtg tcggaccgga ccaatcgcag cgaagtctgg acgcgcttca tcgcggcggc
    26521 ggaacagctg ggcattcccc gcaacccgga cttcaacggt gccgaccagg agggcgtcgg
    26581 ctactaccag gccacggtag gcaacggccg ccgctgcagt gcggccgtgg ggtatctgcg
    26641 gccggtgaag ggtcgcgcca acctgagcgt catcaccggg gcgatggtgc accacatcag
    26701 cttcgagcag cggcgcgcca ccggtgtgcg ctacgaaaag gacggccact ggcatgagct
    26761 gcacgccgcg cgcgaggtgc tcgtctgcgg cggttcgatc aattcaccgc agctgctgat
    26821 gctgtcgggc atcgggccgg cagcccacct tcaggagatg ggcatcacgc cacgggtgga
    26881 cgcaccgcag ctgggcaaga acctgcagga tcacctgcag ttgcgcctga cctaccgact
    26941 caaccgtccg atctcgttca acgaccagtt ccattcgctg gccggcaaag tcggcatggg
    27001 gctggaatat ctgctgcgcc gcagcggcgc catcgcctac ccgacggccc aggtcgggct
    27061 gttcaccaga agcctgcccg agcttgcgag ccccgacatc caataccact tctccaacta
    27121 caccaccgac gaaaaaaccg gcatgccgga ccgctttccg ggcatgacct tcagtgtctg
    27181 tcacctgcgg cccgaaagcc gcggcgagat cctgctgcgc agtccggacc cgggccaaca
    27241 cccccggatc ctgcccaact acctggccac gccggaagac tgccgcgtgg ccgtcgagga
    27301 ggttcgcctc acgcgccggc tggccgccac gcaaccgctg gccgacatcg tcgccgaaga
    27361 gctggccccc ggccccgacg tgcagagcga cgaagcgtgc ctggatttcg cccgctcgaa
    27421 tggcacctcc atctaccacc cggtcggcac ctgccgcatg ggcagcgacg ccgaggcggt
    27481 ggtcgacccg gcgctgtgcg tgcgcggcgt cagtcacctg cgggtggtgg acgcgtcggt
    27541 gatgccgacg ctgatttccg gcaacaccta cgccgccacc ctggccatcg ccgaacgcgc
    27601 ggccgacctg atcctgaaca cccgcccgta ggcgccaacc ccatacccac actgaaggag
    27661 caagccttga aactcacccc catcctcacc ggcctgtccg cctgcctgat ggccgcggca
    27721 gcgcacgccc agtcggtcga caagtacccc gagcggccca tcaagatcat cattccgtac
    27781 caggccggtg gcggcacgga tgtgatgacg cgcatcatcg tcgagaaggt gcgcgagcgc
    27841 accaagcagc ccttcatcat cgaaaccaaa cccggaggca acaccgtcat cggcgtcaag
    27901 gccctgctgg ccgcgcccgc cgacggctac acgctgatga tgaccagcag cgccaccttc
    27961 cacaccgcac ccatcattgt caagaaccct ggctacgacc ccatcgagtc gttctcgctc
    28021 atcagctaca tcgcctatac cccagtggtg ttcgtcatca attcgtcggt gaaggccaac
    28081 aacctgaagg aattcatcga tctggccaag gccgcgccgg gcaagtacgc gctcagcacc
    28141 tacggacgac agctgggcac cgacctgttc cagaaggttg ccggcttcaa actcaactcc
    28201 atcccgtaca agggcgtgga agcggtcaat gccgtggttg ctggccacac ggatgcgatg
    28261 ttcgatggcg tcttcaccgc cctgccctac ctcaagcagg gcaagacgcg cgcactggcc
    28321 gtgatgcagg acaaacgcac ccgcttcgca cccgacattc ccacggtcac cgagctgggc
    28381 tacaagtcca tgcccgacat cccgatctgg tacggcctgg tgggccccaa gggcgtcgac
    28441 cagaaaatca ttgacaagct ggccaacgag ttcaagatcg ccatggagtc gcccgaagtg
    28501 atcgagaagc tggccaacct cagcgtcgaa tccgtcggct cgggcccgca ggccttgcgc
    28561 aagcagatca ccgccgagta tggccactac cgcaagattg ccgacgaact gggcatccag
    28621 cccgaatgac gccgcgccgc gccgatcgac gcccggcccc acggagacac ccatgacaca
    28681 ccccgaccgc ctgagactga gcctgccgct gctgctggct gccccgcaac tgctgtggtc
    28741 cgtgcccgca cgggcgcagg acaaatggcc gtccgggccg atccgcatgg tggtgccgtt
    28801 tccgcccggc ggttcatcgg acatgctggg tcgcctgatg gcagacaagc tcagcgcgct
    28861 gctgaagtcc aacatcttcg tcgagaacaa gcccggtggc accacgcaga tcggcaccga
    28921 gctggtggcc aacgcggccc ccgacggcca caccctgctg atggcctctg ccaccgggtt
    28981 cacggccctg cccaacctgc gcaagctgaa ctacggcatc gacagcttcg agatcgccgg
    29041 cggcgtggcc gactacgtcg ccgtcatggc ggtgcgcaag accttgccga tccgcaccgt
    29101 caaggagttc atcgactacg ccaagcagca tcccggcaag ctgtcgttcg gctcggccgg
    29161 tgaggcgtcg gcgggccaca tctacggcct gacactggcg cgcgacaccg gcattcaggt
    29221 gctgcatgtg ccatttcgcg gttcggcggc ggccgtcaac gccctggtgg ccggcgacgt
    29281 cgacttcatc atcgacggcg ccgtcacccc catggtcaaa gccgatcgcg tgcagccgct
    29341 ggcggtgctg tatcggcgtc gccaccccga gctgcccggc gtgccgacgc tggaggaagc
    29401 cgggttcacc atcagctcca cccgcggttc gggctggggc gtgctggcgc ccaagggcac
    29461 accggcggcc atcatgtcgc ggttgtccga ggcgatccgt caggtgctgc agcagcagga
    29521 cgtgaaggac gcgctggcac gggccaactc ggtgccttcg tggcaggcgc cggacgagta
    29581 tcgacgcagc ctggatgccg accgcaagct gtacgccgag ctgctgccgg cgatcggcat
    29641 tcgcggcaac tgaggcaccg gtcatggtcg acgtctccaa cccggcgccg tgggttgcac
    29701 cgcgccagag cgtccgcgac atcctgcacc cgcgcagcgt ggccatcgtg ggggcttccg
    29761 acagcgaaga caaatggggc gggcgcctgc tgcgctacat gctgcggcat cgccacccgg
    29821 gcgcgctcta ccccatcaac gcccgtgcca ccgagctcat gggcctgccg gcctaccctt
    29881 cgctgcgcga ctgccccggc ccggtggacc tggcgattct gctggtgccg cgcgcgcatg
    29941 tccgcgcggc catcgaggac tgcgtggcca aaggtgtggg ctgcgcgctg tgcatcacgg
    30001 ccgggtttgc cgagaccggc gcggccggcc gcgccgacga acaggcgctg gtcgacctgg
    30061 cgcgcgccgg cggtgtgcgc ctgatcggcc ccaactgcat gggcctgatg aacacccacc
    30121 acgggctgtg tgccaccacc ggcgtggtga tgggcaccct cgacaggctg ccggtcggcg
    30181 gcatcggcat ggccagccag agtggtgcgc tgatgggcgc gatgatttcg cgcggggtcg
    30241 acatcggggc tgggttctcg accacggtct cggtcggcaa ccaggccgac ctcgacctga
    30301 acgacttctt cgaatacctg atcgacgatc cgaagaccga ggtcatcacg ctctacatgg
    30361 aaggcgtgaa ggacgcgccg cgcttcaccg cccttctgga gcgcgccgcc gccgcgggca
    30421 aaccggtgtg catcgccaag tcggggcgca gcgcggccgg cgccaaggcc gccgcctcgc
    30481 acaccgccag cctggccggc gcctggcccg ccttcgaggc gacctgccgc cgctggggtg
    30541 tctacctgtt cgagaccatc tacgatctgc tgcagggcgc gctgctgctg caacgcggca
    30601 agcgggcggc gtcggatcag gtggccgttt tttcgggctc cggcgggggc ggtgcgctgc
    30661 tgaccgacgg tctggaagcg cacggcctcg ccccggccga gctgtcggac gccacccagc
    30721 aggccgtcgc cgccgtgttg ccgtccacgc accgccagtt gccgttcgac ttcggcatgc
    30781 tcaaccatgc ggcagcgccc gatccgcagg tggcgggcgg cgcaatcggc atcgccctgg
    30841 accgcgccct gcacgacccc gcggtcggcg ccggcatcct gctgctgacc acccagcccg
    30901 gccaggagct ggtggtgcag gcggccatcg ccgccgccga ccgctcctcc aagcccctgc
    30961 tgttcgtcca gggcgccggc aaccacggcg acgtggcgcg ccagatgttg cgcgcggccg
    31021 gccacggcta cttcgacagc ccgcacgacg cgctgaaggt gctgtcggtg ctggcgcggc
    31081 gcaagccgcc accgcccagc gcgccaccgc agcccggcgt cagcgtcgcc gccgatctgc
    31141 cggccggttt cctgaccgaa cctcaggccc gccgcctgct ggaaggggcg ggcatcccga
    31201 ccacacgctg gcggttcgcc gccggcgccg acgccgcggc ccaggccgcg cgcgaacttg
    31261 gcggcagcgt ggccatcaag gcggtcagcg cgcacatcgt gcacaagagc gacatgggcg
    31321 gcgtcaagct cggcctgcag ggcgatgccg ccgtgcgcca ggcctgcgcc gacatcgccg
    31381 cagcggcgcg cgcggccggc gtcgataccc ttgacggcta cctggtcacc gagatgtggc
    31441 gcgccgacac cgagttgatc ctgggtgtgc agcgcgaccc cgacttcggc ccgctggtga
    31501 tggtgggtgc cgggggcgtg ctggtcgagc tgatgaagga cgtgcagctg gcgcccgctc
    31561 cgctgtcgca cgccacggcg ctggcgatgc tgcgacagct gcgttgcctg ccgctgctga
    31621 gcggctaccg cggccgcacg ccggccgacc tggacgccat cgccgacgtc atggtgcgcc
    31681 tgggcgcgct ggcggccgac cccagcgccc ggctgcgcga actcgacatc aacccgctgt
    31741 tcatcgccgg gccgcgcatc gcggcggccg acgcgcgcgc cacgctggcc tgatgcacct
    31801 accaccgaac agcttgcacc tgaagcaacc atgaccgccg tacgctacgc ctccgagggc
    31861 ggcatcgccc gcatcaccat ccaccggccc gaagcccgca acgccatcaa cgacgaggtg
    31921 gtgcagggca tgcaggccgc atggcaacgc ttcgcccagg acgccgacga ccgcgtggcg
    31981 gtgctgcacg gcgcgggcga gcaggccttc acgtcgggcg ccgacctgaa agcgctgccg
    32041 cgcgacatct ggctggccat gcccaacctg tcggtgccgt gcgacaagcc gatcatcgcc
    32101 gccgtcagcg gcctggccgt cggtgccggc gccaccatgg tgatgctggc cgacatggcg
    32161 gtggccgacg aggacgcccg cttcgtctac ccggaggccc ggatcggcgc ctttgccggt
    32221 gtgatggctg gttttccgcc ccgcatgcaa tacaaggccg gcctggaatg gctgctgacc
    32281 ggcgaccccg tgacggccca gcgcgcctac gagatcggcc tggtcaaccg cctggcgccg
    32341 cacggtcagg cgctggaggt ggccatgcag gtggccagcc ggatcgccgc caacgcaccg
    32401 ctggtggtgc aggccatgaa gtcgatcgcg cggcgcacgc tgccggcttc gccgaccgag
    32461 ctgtattacc cgcagcaccg catgctgcaa ggcatcgccg ccagcgccga cctgcgcgaa
    32521 ggcgtggcct cgttcaagga aaagcgcgcc ccgcgcttca gcggccgctg atccccgcac
    32581 tcaaccccaa ggagcattca ccatgaccct cacccgtcga tccctgcacc tgctggcgct
    32641 ggccgcgctg tccggtgccg tcgcgcccgc gctggcccag acgtccaagc cctggccgga
    32701 caaacccatc cgcttcgtca caccgtaccc gcccggcggc tcgtcggacg tcatcacgcg
    32761 cttcatcggc gatggcgtct ccaagatcct tggccagccg gtgatcgtcg agaacaagcc
    32821 cggcgccgcc gccaccatgg gcaccgagtt cgcgtcgcgc caggcgcccg atggctacac
    32881 cttcctggtg gcgcccaccg ccgccctggc gctggcgccc tacctgatga agaacgtcaa
    32941 gtacaccttc gagagcttcg acccggtggc caagatcgct tcctcctacg gtctggtgac
    33001 ggcccgcaag gacgcgccgt ttaccgatta caagggcatg ctggcctacg ccaaggccaa
    33061 ccccggcaag ctcacgttcg ccaccaacgg cgtgggctcc atcgtgcaca tgaccgcggt
    33121 gctgctgcac aagcacgccg gcgtgcagct cacccacatc ccgtacaagg gggcgggcga
    33181 gtcggtgatc gacctgctgg ccggccgcat cgacctcatg tacgacccgg ccacggcacc
    33241 gcgcgtgaag tccggcgacc tcaaggggct gggcaccacc agcggcatcc gcaaccccga
    33301 actgcccaac ctgccgacgc tcaaggagca aggctttggc gacggtggct cgttcagctg
    33361 gtttgccatg ttcgcaccga agggcacgcc ggcgccggtg gtcaacaaga tggccgacgc
    33421 cgtgcgacag gtgctggaag cgccggaagt caaggccaag ctgcagctgt cggcgctgta
    33481 ccccaactac gaggacccgg cgacctttgc caagtcggtc aggaacgacg cggaaacgct
    33541 gcgcaacgtg atccagcagg aaggcatcaa gctggagtga ccttcaaagc ggccgttgac
    33601 cgttggtcag cttcgcaaag ctgggacgaa cgcagggatg ggcggcctgg tggatcgtca
    33661 gctggtgagt caggctcagc tgcgccgcca gaccctgcag ggatgcgcct gcggacgcac
    33721 aacagccgca gccagccgcc gcgttcagac tcagcccagc gccagtcgtg ccgcccgcac
    33781 cagggcccgc gcgtcgacgc cgaagaagcg ccgcagcgcc gcacgggtgt cgctgcgccc
    33841 gaagccgtcg gtgcccagcg tgaggtagct gcgcccttcg ggcacgaacg cccgcacgct
    33901 ctccggcacg gcgcacacgt agtcggtggc ggcgatgatc ggcccctggg tgtggtgcag
    33961 ctgctgggtg acccagggcg cgccgggcgt gtccgcgccg gccagacggc gctgctggca
    34021 ggccaggcca tcgcgcgcca gttcgctcca gctggtgacg ctgagcacgg tggcggcaat
    34081 gccctgacgc accagcagct cggcggccag gatcacttca gtcaggatgg cgccggagcc
    34141 gagcaaggtc accgttgggg ccgtttctgg cagttcgcgc ccgtccggcg tgcgccgata
    34201 gctactaaat agatagcacc cacgcaacac ccctggatgg gcatccgccg gcagcgcggg
    34261 ctgggcgtag ttctcgttca tgagcgtgac gtaatagaac acgtccttct gctccaccat
    34321 catctcgcgc atgccggcgt ccacgatgac cgccatctcg cccgcaaagg ccgggtcgta
    34381 ggccttgcag ttggggatgg tggcggccac caggtggctg gtgccgtcct ggtgctgcag
    34441 gccctcgccg cccagggtgg tgcgcccgct ggtggcgccc agcagaaagc cgcgcgcgcg
    34501 ctggtcggcc gcagcccaga tggcgtcgcc cacgcgctga aagccgaaca tcgagtagta
    34561 gatgtagaac ggcagcatgg ccaggccgtg cacgctgtag ctggtggccg cggccgtcca
    34621 gctggccagc gcaccggctt cgctgatgcc ttcttccaga atctggccgt cgcgcgcctc
    34681 gcggtaggac aggatggagc cgatgtcctc cggcgcgtag cgctggccga cgctgctgta
    34741 gatgcccacc tgtttgaaca ggttggccat gccgaaggtg cgcgcctcgt cggcgacgat
    34801 gggcacgatg cgcggcccga gctgtccatc cttgagcagg ttacccagca tgcgcacgaa
    34861 ggccatggtg gtgctcatgt ccttgccgtc ggcggccacg gcaaactgcg cgtagctggt
    34921 cagggccggt acggccaccg cctcacacag cgtctgacgg gccggcaacc acccgcccag
    34981 ggcgcgccgg cgctgcagca ggtactgcag ctcggcgctg tcgaccgccg gcttgaggaa
    35041 ctccaggttg gcggcctgcg cgtcggtcag cggcaggttg aagcggttgc gaaacgcgat
    35101 caactcctgc tcatcgagct tcttctggct gtgcgtggtc atcttgccct gtccggccgc
    35161 gcccatgccg tagcccttct tggtgtgcgc caggatcacg gtgggctggc cgcggtgggc
    35221 atgggcggcc gcataggcgg catggatctt caccaggtcg tggccgccgc gcttgaggcg
    35281 gtcgatctgc tcgtcggtca tgccctgggc cagttgctgc agctcggggc tctggccgaa
    35341 gaagttgtcg cggttgaagc gcccgtcctt ggcggcgaag gtctgcatct ggccgtccac
    35401 ggtctcgccc agcgtgcgca ccagggtgcc ggtgaggtcg cgcgcgaaca ggccgtccca
    35461 gtcgctgccc cacagcagct tgatgacgtt ccagccgctg ccggtgaaca ggcgctccag
    35521 ctcgtcgacg atgcggccgt tgccgcgcac cgggccgtcc aggcgctgca ggttgcagtt
    35581 gaccacccag accaggttgt ccaggccctc gcgcgcggcg agcgtgaggg cgctggtgct
    35641 ctcgggctcg tccatctcgc cgtcgccgaa cacgccccac accttgcgac cggcacagtc
    35701 cagcagaccg cggtcggtca ggtagtgcat gaagcgggcg tggtagatgc tgctgatcgg
    35761 gccgatcccc atcgagccgg tgggaaactg ccagaagtcc ggcatcaggt aggggtgcgg
    35821 atagctgcac aggccgcgcg cgccggcgcc gggcgccgtc agctcctggc ggaagtgcag
    35881 caaatcctgc tcggacagcc gcccttcgag aaaggcgcgg gcgtagacgc cgggggcgct
    35941 gtgcggctgg aagaacacca gatcgccccg gtgctggccc ggcgccagac cctcgcgggc
    36001 gcggaagaag tggttgaagc cgacctcgaa cagatcggcc gcgctggcgt agctggcgat
    36061 gtggccgccc agctccccat aggcctggtt ggcgcgcacc accatggcca gcgcgttcca
    36121 gcggatcagc gagccgagcc gctcctcgat cgccagatcg cccggaaaca ccggctggtc
    36181 ctgcgccgcg atggtgttga gcgccggcgt gttgagctcg ggccgccagc ccagctggcg
    36241 cgcgcgcgcc aggcgcgcca gctcgtccag catgaaacac gcgcgcgccg ggccctgagt
    36301 ggcgatcagc gcctcgaacg cggcgcgcca ttcggcggtt tcctgcgggt cggcatcggc
    36361 gggcagggac gcggaagacg ccagggcctg ggaatcgatc ggagcatgca tggtggcgac
    36421 tcagtggact gcacagaaaa accatcgcgg cgcgccggca cacaccgtcg ccgaccgcgc
    36481 catcgtcggc atgatcccgc agcgcctgtc ggtggtcaat gcgggccggc ggcaccggct
    36541 cagaacacgc tgctcaggct gcgcgccccg ccacgggcgg cgccctccgg cgccagcgcg
    36601 tggtccagcg cctgaccgat gtcggccagg atgtcctcca cgtcctccag gccaatggac
    36661 agccgtacca gcccttccgg aatgccatgc gcggcgcgtt cttcgggggt gtaggtcgag
    36721 tgcgtcatgc tggccgggtg ctgcgccagg gtctcggcat cgcccaggct gacggctcgc
    36781 gtcaccaggc gcagggcgtc catgaagcgc acacctgcct gcaggccgcc gtgcagctcg
    36841 aacgcgatca tgccgcccat ctggcgcatc tggcgctgcg ccagcgcgtg ctgcgcaaag
    36901 cttggcagtc cagggtagtg caccacggcc gccgccggat gtgcggcgat ccgctcggcc
    36961 acctgctgcg cgttctgaca gtggcgatcc atgcgcagtg ccagggtctt gagaccgcgc
    37021 atcaccaggt gggcgtcatg ggccgacatc acggcaccgg tcatgtcctt caggccgtac
    37081 aggcgcacgc gctcggccag ttcggcgtcg gcaaacacgg ccgcgccggc catcaggtca
    37141 ccgtggccgc ccaggtactt ggtgaccgaa tgcaggctga cgtcggcccc cagcaccagc
    37201 ggctgctgca ggtagggcga gcagtaggtg ttgtccacca ccacccgcgc tccgcgggca
    37261 tgcgacagac gcgagatggc ggcgatgtcg gccagtcgca tgttggggtt ggcgggcgat
    37321 tcgatgtaga ccacgcgtgt gcgctcggtg agcgccgccg ccacctgctc gggatcggtc
    37381 atgtcgacgt ggcgcaccgt gacgccgaag tgcccgatgc cgtggtgcag gaacgaaaag
    37441 gtgcagccgt acagcgtcag gtcggcaagc acctcgtcac ccggcgtcag catggaccac
    37501 agcgtggccg tgatggcgcc catgccggag gcgaacacca ccgcacccgc gccctgctcc
    37561 aggctggcca gacgcgcctc cagcagcgcc agcgtggggt tggcgatgcg gctgtagatg
    37621 tagcccttct cctcgccggt gaagcagcgc gcgccgtagc ccgtgtcggg aaaggcgtag
    37681 gtggccgagg tgtggatggg cggcaccagc gcacccagat gctcggccgg gtcgtagccg
    37741 aagtgaatgg cgcgggtgct gaacccaccc gctcgatcaa gggacctggt catgaaagaa
    37801 tctcctgcag cgtcaaatca gaagcgtatt ttttcctttt acgacgggca agaattggct
    37861 atcttgatgc ttttatggca gcacgatagc agtttgtttt tttttcattg ttgtttgggg
    37921 caaatcatgt cttcaccctc cggctccttc gatcatgtgg atcgcgccct gctcgaagtg
    37981 ctgcagcgcg acggccgcgc cacggtgggc caactggccg aacgggtctc gctgtcgccc
    38041 tcgccctgct ggcggcgcgt gcgccagctg gaagaaagcg gcgtcatcag cggctaccac
    38101 gcccacctcg accgtcggcg catcggctat ggcgtgctgg gcttcgtcca gttgaacctg
    38161 cacgatcaca cgtccagcat ctgcgccgcg ttcgagcgtg aggtgctggc gctgccgcag
    38221 gtgctggcct gccacaacct gtccggccgc cacgactacc agctcgaagt ggtggcgccc
    38281 gatctggagt acttcgccga gttcgtgcgc agccgcatcc gcagcctgcc tggcgtgcgc
    38341 gagatttcca ccagcttctc gctcaaggag atcaaacgca ccattgccct gccgctcgac
    38401 cactgaaccc aggctgaatc ccaccctgca tacactgcgg ccattcaagg cgtgcaagga
    38461 ccacaaacac catgacggac ttcgacttcg accttttcgt catcggcggc ggctcgggcg
    38521 gcgtgcgggc ggcgcgcttc gcggcgcagc gcggcgctcg cgtggcgctg gccgaaagcg
    38581 gtcgcatggg cggcacctgc gtcaacgtgg gctgcatccc caagaagctc tacagctatg
    38641 gcgcgcacta cgccgaggcc ttcgaggagg cgcgcggcta cggctggcag ctgcccggcg
    38701 cgccacggct ggactgggac gcgctgaagg cccgccgagc gcaggaaatc tcgcgtctga
    38761 acggcatcta tgaaggtttg atgaccggcg ccggcgtgcg gcgtctgcaa gggcatgggc
    38821 ggctgctcga cgcccacacg gtcgacgtga cggcgccgga cggcggccgc agccgccacc
    38881 gcgcgcgcca catcctgctg gccaccggcg gcacgccggt gcgacccgag ctgcccgggc
    38941 acgagctgat cgtcacttcg gacgacatgt tcgacctgcc gcggtttccg cgccacctgg
    39001 tggtggtggg cggaggctac atcggctgcg agatggccag catcttccac ggtctgggcg
    39061 cgcaggtcac cctgctgtac cggggcgagc agatactgcg cggcttcgac gacgaggtgc
    39121 gcgacttcac cgcggccgag ctgcgcaagg ccggcatcga tctgcgcgtg cgcaccgagg
    39181 tcacgcgcat cgaggccgcg cccgacggca cgcgccgcct gaccctgaac gacggcggcg
    39241 tgctgcaggc cgacgtggtg ctctatgccg tcggccgccg gcccaacgtg gcgggcctcg
    39301 gcctcgaagc cgtgggcgtg cggatgaccg ccagcggcgc cattgcggtg gacgagcact
    39361 tcgccaccgc ggtgcccggc ctgtacgccc tgggcgacgt gcgcggcggg ctggagctga
    39421 ccccggtggc gctggccgag gcgatggccc tggtcgacca cctgttcggc cccctgcccg
    39481 gccaggccag gcgcaccatg gactacgagc acatcccgac cgccgtgttc acccatccgc
    39541 ccatcggcac cgtgggcctg accgaagccc aggcgcgcgc gcgggtcggc accatccgcg
    39601 tctatcgcag cgacttccgg ccgctcaagc acaccctgtc ggggcgcgat gagcgctgtc
    39661 tggtcaagct gatcgtcgat gacgccagcg accgcgtgct gggcctgcac atggtcggcg
    39721 ccgacgccgg cgagatcgtg cagggctttg ccgtggccct gaaatgcggc gccaccaagg
    39781 cgcaattcga cgccaccctg ggcatccacc ccaccagcgc ggaagaattc gtgacgctgc
    39841 gggacgtgac gcgcacctga gcagtgcgca tgaacccctc gtgtccatct tctgaaccgg
    39901 ccagcgctca gcgtcgaccg gccaggcgcc ggccatcgcg caggttcagc gccagcaacg
    39961 tgatgttggt ggccccggcc accaactcca gcccctgcag cgcatagaag ccagcgtcca
    40021 gccggcccgc gctggcccat cgcgccagca gcaaggcact gggcagcagc accagcaggc
    40081 cgttgaccgc caccatcttc atgcgccgct gcttggcttc gaccagccgg ccgcgccgcg
    40141 agcgcgccaa cgagaagcca ctgccgccgg ttgcggccat cgccgggatc agcacccaca
    40201 tgccggccac caccatgttc ttcacgaaca ccacgctggc ctggtcgagc agcagctcgg
    40261 cgacgaccgt cgacagccag aacgtgacaa tgcacagcag cgccaccaga ccgaacacac
    40321 catgcagttt cgctttcatg tcgcatcctc caagtcggcg gtggcgctgc ccagcacgtg
    40381 ctcgatgtgc tgcagcacct cggtggcttg acgcgcctgc gttgccgaaa tctgtctggc
    40441 cagctgcgcg acctgatcgc gccacagcgc atcggcctgc tcgtagcact tgcgcccgtg
    40501 tggcgtaagg caatagagcg gcgagcgccg gtgcgccggg ttgggatcga cctgcaccaa
    40561 gcccagcccc tgcaacacgc ccagttgctt gagcgcgccc tggcgagaca cgcccatcgc
    40621 cgcacccacc tggggcgcgc tcagaggcga gtccgccagc gccagcgcac ccagcatctg
    40681 ccagcgcgcg ctgctcagcc ccagcgtcgc cacccgtcga tcaccccagt gcagcaaggc
    40741 gccattcagg cgaaacaccg ccagcgcaag tgcgctgatc gcatcgatct gcggcagggc
    40801 attcatggtt caggcttcgg ccgtgatatt gacaaccagt ttacccattt gggaaaccgg
    40861 ttgtcaatgc gtgacggaga ccgaagccgc ggcgcgccgg ctgacgcgcc ccagcatcag
    40921 ccagaccagc agcgccacca cgatcagcgc gccgccgccg gcctgccaga aggccaccgg
    40981 cgaatgctgc gcggccagcg gcccgacgcc gtggtacgcc agcaggtagc cgccggccag
    41041 gcccacgccc cacagcagca caccgtagac cacgaacggc gccagcgtga cgcggtagca
    41101 gcgcagcagg aacatggcca ccgtctgcaa ggcgtcggcc gcatgcagca gcgccaccca
    41161 gcccagcagc cgcgtcgcca gcagcaccac cccggcctgc gccgaataga ccgccgcgat
    41221 ccacggtttt gcaacaaaaa ccatagctgc cagcgcaagt cccaagccgc ctgcaagcca
    41281 gaacgactgc atgacgacgg cacgcgcccg cgcctcgtcg ccggccccgc gccaccagcc
    41341 cacgcgggcg ctggaggcca ccgccagcga cagcggcgcc atgtacatca gcgcggcgac
    41401 gttggcggcg atctggtgcg ccgccgtcgc cgtggtgccc agccgcgcca cgaacagcgc
    41461 catcagggtg aaggacgtga tctccaccag catggtcagc gccgccggca cgcccaggcg
    41521 cgcaaaggcc gccagcgtcg ggccatgcgg cggttcgggg cgccgccaca gttgcagcgg
    41581 ctcatacagc ggctgggtgc gcagcagcca cagcgccagc cccagcaaca ggccatgaac
    41641 ggcgaaggtg gcccaggcgc agcccaccac gcccagggcc ggcacaccgg cgccgccaaa
    41701 ggtcagccac accgacagcg gcagcttgac ggccagcgcc agcacctgca accagctcac
    41761 cagctgcggc cggcccagcg cctggttgag cgtgccgaag gcacgaaaca gcagtgccgc
    41821 cggcaggctc cagcccagca cggccaggta gtcgaccacc accggctgca acccggccgg
    41881 cacgtcggtc cagcgcaaca gcggctccgg ttgcagcagc accgccagac ccagcagact
    41941 ggccgcggcc cagatgtaca gcgcctgccg caccgagccg ccgacctcgc ccagccggcg
    42001 cgcgccgcgc agctcggccc agatcggcag cagcgccgtg aacaggccca tcagcgatac
    42061 atgcacggtg acgaacacgg ccgcgccgat cgacagcgtg gccagcgaca ggtcggaatg
    42121 ccgccccgcc acgatggtgt cgaccaccgc aaaacccatc accgcgacct ggccgacgaa
    42181 caccgcccag gcgtcgccgg cgatcacccg cagatcgctt cggtcggcac gcgcggcagg
    42241 ctggcaggag tcgctggcgg tcgtcacggc gcgcattgtg gcagtgaccg tgcgccgcaa
    42301 ccggacggcg agccacccgg gcgacagggc gggcgcgctg cggcgccagg ccatcgccgg
    42361 cgcacccagg cgctacgatg gtgcctcgcg aggagagcac cccatgaccg acctgagcaa
    42421 gatcacctgc atcgaagacc tgcgcgtgct ggccaagtgc cgcgtgccgc gcatgttcta
    42481 cgactacgcc gattccggca gctggaccga aggcacctac cgcgccaacc aggccgactt
    42541 tgcacccatc cagctgcgcc agcgcgtggc catcaacatg gaagggcgca gcaccgccac
    42601 caccatgatc ggcgagccgg tggccatgcc ggtggccctg gcccccaccg gcctgaccgg
    42661 catgcagcac gccgacggcg agatcctggc ggcgcgcgcg gcgcgggcgt ttggcgtgcc
    42721 cttcacgctg tcgaccatga gcatctgctc gatcgaggac gtggctcagc acgccggtcc
    42781 gggcttctgg tttcagctct acgtgatgcg cgaccgcgac ttcatcgagc gcctgatcga
    42841 ccgcgccaag gccgccggct gcggcgcgct ggtgatcacg ctggacttgc agatcctcgg
    42901 ccagcgccac aaggacatca agaacggcct gtccaccccg cccaagccga ccctggccaa
    42961 cctgatcaac ctggccacca agccgcgctg gatcatgggc atgctgggca cgccgcggcg
    43021 caagttcggc aacatccacg gccatgtgaa gggcgtgtcg gacatgagca acctgggcgc
    43081 ctggacggcc gaccagttcg acccgcgcct gaactggggc gacgtcgaat ggatcaagaa
    43141 gcgctggggc ggcaagatca tcatcaaggg catcatggag ccggaagatg cgcgcctggc
    43201 ggtcgactcg ggcgccgacg cgctgatcgt cagcaaccat ggcggccgcc agctcgacgg
    43261 cgcgccctcg gccatcgccg ccctgcccgc catcgtggac gccgtgggca cgcagatcga
    43321 ggtgcacatg gacagcggca tccgcagcgg ccaggacgtg ctcaaggcct gggcgctggg
    43381 cgcgcgcggc acctacatcg gccgcgcctt cctgtggggc ctgggtgcgg ccggcgaggc
    43441 gggcgtcgcc aaggcgctgc agatcatcca caaggagctg gacctgacca tggccttctg
    43501 cggtcacacg cgcatcgaaa gcgtggatcg gcgcattctg cggcccggca cctttcccac
    43561 cccctgaacc cgaggcgccc atgctgcacc cccaagccca ggccctgatc gatctgctgg
    43621 tggcccatgg cctgccaccc acccacacgc tgacgccggc cgaagcgcgc gccttctacc
    43681 gcgagcgccg cgcgctgacg cagcccgccc cgcccgacgt ggccgagctg cgcgagctgc
    43741 gcctgccggg cccggcgggc gagctggcgg cgcgcgccta ccggccgctg ggcgccaggc
    43801 ccgaggccgt gctgccggtg ctggtgtact ttcacggcgg cggctgggtg atcggcgatc
    43861 tggacacgca cgacacgctg tgtcgcgagc tggccaacgc cagcggctgc gccgtggtgt
    43921 cggtggacta ccggctggca ccggagcacc cgtttcccgc cgccttcgac gacgcgctgg
    43981 cggccacgcg ctgggtggcg cgccacgccg gcgagctggg cgccgacccg acccgcctgg
    44041 cggtgggcgg cgacagtgcc ggcggcaacc tggcggccgc cgtggcgctg gccgcgcgcg
    44101 acgaacgcga cgccggcggc ccgctgccga tcgcctttca gctgctgatc tacccggcca
    44161 ccgacatgcg ccgtggccat ggctcgcacc agcgcaacgg ccagggctac atcctcacgc
    44221 gcgagaccat ggactacttc atcggtcact acctgccgcc cggcacctcg cccaccgact
    44281 ggcgcggctc gccgctgctg gcggccagcc acgtcggcct gccgccggcg ctggtgctga
    44341 cggccggctt cgacccgctg gtggatgaag ggctggacta cgcgcgcgcc ctgaccgccg
    44401 gtggcagccg cgccagctac gtgtgcttcg gccgccagat ccacggcttc atcaccatgg
    44461 gccgggtgct cgacgaagcc aacagcgccg tcgccctgtg cgccaccgag ctgcgccgcg
    44521 cgctgacgcc gtgacgcccg ccgcgccgcc ggctcacagc cagcgccgca cgcgcgcctg
    44581 gtagtcgatg tagtcctgcc cgaagcgctc gcgcaggatg cgctcctcgg ggccgatctg
    44641 cagccggttg agcagcagca ccgacagcgg cagccccagc agcgcggccg cgctgcccag
    44701 ccacacggcc cagccggcca gcaccaccag catgcccagg tacatggggt tgcgcgtcca
    44761 gcggtacacc ccgcgggtca ccacggtgcg cgcgcgctgc ggcgccagcg ggttgaaggt
    44821 ggtgcgcgcg cggcgaaatt cgagcagccc ggccagcgcc accagcgcgc cgatcagcgc
    44881 cagcccgagt gccagcgccc acacccacca ggcgccaccg gccggccaca gccgcgcggc
    44941 tggcacggcg ccggccagcc aggccatcac ggccgcgcag gccaggtcga tcaccggcgg
    45001 cgggatccgg tgttcgtacc agcgcctcat cgcggccctt ccaaactatt gaaaacagag
    45061 cacccggcgc acgtcctact tgcgcagcag ccctttcagg gcagtctgca ggcggcgtgc
    45121 cgtggcgtcg tcgtgcgggc cggccagcac ggcggcggtg tcctgacccc aggtctgggc
    45181 cggcgacggg tcgttgcgcc gcgtcagcaa ctgcagttgc agcgcgcgcc gcgcctgcag
    45241 atgttcggcc ggggtcggca cctcggccgc catttccagc cgcagcaggg cttcggccgc
    45301 cggcgcgccg gcgccgccgc cgatggcctg cgtccaggcg ttgcgcagcg ccggcgtgac
    45361 gccgcggccc agctcctgca cgccgggcac ctggtcgggc tggcgcgcct gccaggcgcc
    45421 cagcagctgg gtcagcgcct cgccatgggc ctgcgcggcc agcttgcgca gctgcgcctg
    45481 ggcctgctcg acggcgtcgc gctgggcgcg gaaggcggcg tcgcccagac gcgggccacg
    45541 cgcctcgtcc gagcggcgcg ggccgcgagc gtcgtcgaag cggcgcgcac cgcgctcgcc
    45601 cgcacggccg gccgcacctt cgcgccggtc acccgcgcgc ccgtcgcgac cagcggggcg
    45661 gcgctcgccc gggcggccgg ccggcgccgg ctcggccttg cgggcaccgg ggcggtcgtc
    45721 gccgcgcatc gccaccaggg gcttggcggg cttgggcgcg ggggccggcg cggcgccctc
    45781 ggcgggggcg tccggggcgt cggtgggcac ctcggttgct tctatttcga tagcttctgg
    45841 cgcgccatcg gcgggcgctg cggccggttt ttcttcagat cccggggcag gctgggcacg
    45901 cgtggcggct tccagctggg ccatggcggc gcggatgcgc tgcgcgtcgc cgctggcgtt
    45961 ggccgcctcc agcgccttgg cggcttcgag cacggcgcgg tcgtgcgcgc tcatcgccgc
    46021 ctgcgccttt tcgcgctcgg cgctcttgcg gttgaaggcc tcgtcgatcg gcttgcggaa
    46081 ggcgtcccac agcttctgct cgtgcttgcg atccagcggc acggcctggg cctcggcctg
    46141 ccagcgctgc tgcagcgcgc gcacggcgtc gatgcgcagc tgcggcgcgg cgcccagcgc
    46201 ttcggcctcc tggatcagcg cctggcggcg cgcggtgctg tgcttctgca tgttttcgag
    46261 cggcagcgcg gcggcgtgga tggcttcctt ccagcgcggc tgcagttcgg cgaactgctt
    46321 ttccgacagg tggccggcgt tgcgccagcg ctcggcgaac tggtggattt ggcggttgac
    46381 ggccttccag tcgccgccct cgccggcgtc ggcgtggctg gcggcccagg cgcgcacttc
    46441 gtcgatcagt gcctcgcgct gcgccttgtg ctcggccgcc tgggcgcgca cgccggcgat
    46501 ccactcgtcg acgaacgcat gggcggtgct gacggcgcgg tcgaagcgct tccacagggc
    46561 gtggttgggc ggcccgccct ggtcggcggc tttccattcg tcgcgcagct tgcggatcgc
    46621 ctcctgcagc ttgcgaccgc ccatggcggg cacccactgc tcgtcggcgg ccagcgtggt
    46681 ggcgggcaac ggacgggcgg cgggttcggc atcgttttct gccccggcgc ttgcagggtc
    46741 tgcgccggca gctgtgcttt cgatagcatc cggttgaccc tctggcgccg tctgctcggg
    46801 cgccgccggg ctggcggagg cttcgggggc ggcggggacg gcggtgggtt cggtggcgct
    46861 ggcctggcca gactcggcct gcgacggcgc cggcgcggcg tcgggcgcgc cctgggccgg
    46921 cgccttcttg cggcgcttga ccagcagggc ctcggcgcgc gccaccagct gctcgcgcag
    46981 cttgtcggcg ctccagcgct gccagccttc cagctcgccg gccgacacca gcacggcgtg
    47041 cacgcgcgct tccagcgcgt cgtcgatcag gcggccgtgc gtcttgagcg actggcgcag
    47101 cgcctgggtg gcgctgtgca tgttcttggt gtggccggcg gtcaccgcct gctccagcag
    47161 catgacgccg gcttcgaccg ccttgtgggc ggcggcgcgc tgctcggggt cgatgcgcgg
    47221 ccgcgccggt ttgtcggctt cggcggcggc ggccggcgcc tcgccgcggg cggcgcgcag
    47281 ttcgtcggcc cacaccggca ccgaaggcag cggcgccgca gcgtcggcgg ccgccgcctg
    47341 ggcctgtgcc agcgcggcgc cgaaggcgtc ccacacggcg ctcagctggg tctgtgcggc
    47401 ctccatctgc ggcggaaagc gcagatcgac gctgggccac tgcgcgtcgg ccagcagggc
    47461 ggcggcctgc tggcgccact gcgcgacgtc ggccgccagc ccgacctgct gcgcctgcgc
    47521 gtcctgccac ggcttggtgg acagcagctc gatgcgctgg gccagcaaga tggcggcctc
    47581 gcgctgcacc atgacgcgct gctgcaggtc ttccaccacc ttgacgcgct gcgccagctg
    47641 ggtgcgcagg ccggccagcg gctcgcgcga cagcggcgcg ccggccttgg cggcgtcgcg
    47701 ctgccaggcc agggcgtcgg cgatgttcag gcgcgccgcc tgcagcagcg cctgggcctt
    47761 ggccgcccat tcggcggcca ccgcttcctg gcccttggcg cggcgcagct cgtcgagccg
    47821 ttcgcgcagc gcgcgggcgg cgcccttgtc cttggccgac aactccttga acacctcctg
    47881 catctgctcg ggcgtcggcc cgctggccag ccagtcgcgc acgcgctggg cgcgctcgcc
    47941 cgaggtggcg gcggaaaaca cgccgccggt cagcgcgtcc agcgggtggg cctcggcggc
    48001 cggccgggcg gggctgtccg gcgcgggttg cgggtcgttc gattcgcgtg agaaggggaa
    48061 catgctgaca ggtgctcttc gacaaaaggc cgagcgcgcc gacctggcgc gcccgatggg
    48121 cgtattttcg ccgggaattg gccgctcgcc gatcgcccca ccgatccggc caccgggaag
    48181 gcggtcgcag ccggccgggc ggccgcgccg ccaccggcct cagcgcgtgg ccagcttgag
    48241 ttcggcgtgc ggcgtccagg ccggcgcggc ggccggcgcg gaccccgaag tggcggccga
    48301 cgcggcgctg gccggcgccg ccgcggccgc gtccgccgcc gcctgcgcgg cgcccagaaa
    48361 cagccgatcg gccggcgccc cgtgcgccgc caggtagtcc ttcaccgcca ccgcgcgctg
    48421 caccgccagc tggcgcatgg cgtcctggcc gacttcgatg tgcgccagca gcagcgcctc
    48481 catctcggct tgcggcacgt cgcgcagcag gccgatggcg ttgcgcggcc ggcccggcac
    48541 gtcggcgcgg cggtacaggc gccgcagcag gcgcaggcgc tgctcggcgt ccatcgcatc
    48601 tgttgcatcc gacgcggccc cagcgcccgc cggatcagcg ccggcagctc ctgatggcat
    48661 agcatccggg gccgggctgc ggtcctcggc cgggttggcg gcggcgctgg cggcgggcgc
    48721 ggcggtggct tcggcgcgcc gctcggccgc cagcagggcc tgcaggcgct ggcgccgcca
    48781 gccctcgcgc tcgggcgcca gacgggcatg gccgaccacg gtcagcgtca gctgcgggcg
    48841 ctcggccagc gccttggcca cgcggtcgag ctgctggcgc gccctggcat cgagccgggc
    48901 gctgccgggc gcgaaggcga cctgcgacag atcctcgcca cccgcgccgc ccagcgcgcg
    48961 tgccagcagg gtgaagggcg cggtgatggc cttgccgatc aggttgagga tggccttgac
    49021 gatcaccggc ccgagcgaaa actgcgggtc gttgagcgag ccgctgatcg gcaggtcgag
    49081 gtcgatcacg ccctggctgt cggacagcag ggcggtggcc aggcgcaccg gcaggctggc
    49141 cggcgcgccg gccaccggct cgctgaactg cagctgcttg agcaccagct ggttgctggc
    49201 cgtgagctgg ccgtcgggct ggatgcggta gttcacgtcc atgctcagct tgccgcgctc
    49261 gatgccgtgg ccggcgtagc gcaccgcgta gggcgacagc ggcggcagct ccagctcgct
    49321 cacctggccg cggatgtcca gcgccagcgg cgtcgccagc gggttgagcc ggccgctcac
    49381 ccgcagctgg gccgagcctt cggcacggcc gctcaggttc agctcggcca tctgcacctg
    49441 gccgtccggc gccaccgagc tgaaggcgcc cagggtgccg gccagctcgc tcaggtcggc
    49501 cgagtagttg gggcggatga agcggtcggc aaaatccacc cgaccacctt cgagccgcac
    49561 cggccccacc cgcacgaccg gcgccagcgc cgccggtgcg gcggccaatg gggcgctggc
    49621 gcccgcggtg ggtgcgccgg cagcgatcga atgggtggtg ccgctgaatt ccccggcggc
    49681 cggcatggcg cccggcgggg tggctggggc ggcgccgggc gagccgcccg cggcgtgctc
    49741 ggacttgagc acgtcctgca ggttcaggcg gccgtcgggc aggatcacga cgcgggcgaa
    49801 gaagtccgac agcagggtct cgccgatccc gagctgcgcc ggctggccgg gatccagccg
    49861 caggtcgacg ctgcccaggc gcagcagctt ccagctcagc agctcctcgc ccaggccgcc
    49921 cgagcgagcg ccggcgcggt cggcggccac cggcgccagc gcggcgcgcg gcgcctgggc
    49981 ggcggcggcg ggcgtgaccg aggcggccga tgtgacgggc gtggccggtg tggccggtgg
    50041 ggtggtgtcg ccggccgccg gggttgacgc cgatgccgac gccaccacgc tgcccggccg
    50101 gctgtgggca cgcagctctt ccaggctggc ctcgccggtc acgcgcacgc gcgccccctg
    50161 ctcggtctgc gccagctcga gctggccctt gaaactggcg tcggcacgca gcagatcgac
    50221 gttgagccac tcgccgaagt acggctgcag ggcatgcagg ggcaggcgct gcacgtcggc
    50281 cgtgccctgc agcgccagcg gcgacagcgt cagctgaccg cgccagctca gccgccccgg
    50341 ctcgctgcga ccggcgcgca gccgcgccgc cagctgcagc ggcatcggcc gccggccgtc
    50401 cagctcgaac tggcgcaggc ccagcgtcag ctgcgtcagc tcggcctgca ccgggccgtc
    50461 cgccaccgcg gcgtcgtgcc agccgagtat gccgtcggcc agcgtcagct catcgagcgt
    50521 cagcttccag gccggcgccg cggcggtggg cgcgggcgcg tcggccggca gcggctggcg
    50581 cagccagcgc tcgaccatcc agcgcccgtc ggcctcgcgc gccagccgcg ccagcggcgc
    50641 gcgcagaccg agccgcgcca cgcgcacggc gcgcgccggc cagtccaggt gcaaccgctg
    50701 cagctcgatc ccctcggccg ccaccaggcg agcgtcgtcg ggcagcgcac tgcgccggcg
    50761 cccgcgcgcc ggtgccggcg cggcgtcggg gtcggtcagg gccagctggc tcagcgtcac
    50821 ctgttcggcg ctgagtgtga gttgcgccgg cgccccggcc tgctcgggcg cggcccagtg
    50881 cagcgccagc cgggccgcca gttgaccgtc caggcgcggc ttcagcacgc cggccaggta
    50941 gggcgcgccc agggccagcg gcaggccatc gatgcgcgcc tgcagctcgg cctgccggcg
    51001 cgtggcctgg ccctcgaagg ccagggtcgc cgcgccctgg ccggcgtccg gcgcaccggt
    51061 cagctgggcc gtgccctccc agtgcagcgg ctggtccggc ggccaggcca gctcgcgcgc
    51121 ggccagctcc aggtgttgca gctgcaggcg cgcggccggc gcgccgtgcg cgctggtcga
    51181 ggcgtcgctg aaatccagcg cgccaccttg cagcgccagc cgcccgatcg ccagccgcca
    51241 gccgtcggcc gcccctgcgg ggccagccga ggcgctcgcc gatgcagggt cggatgggcc
    51301 tgcagagccc gccggatgtg cgccagcagc tattgaatca gtagcatccg gggcgccctg
    51361 ggcaagcagg tcgagctggc cgtcggcgcg ccggtgcagc gccagccgcg gcccgcgcag
    51421 ctcgaccgag cgcagggcca gccggcgctc cagcggtcgc agctcgtcca gttcgagttc
    51481 gagcgcgtcg aaggccagcc gctcggcccc ctgggcatcg accgccttca gcccgcgcag
    51541 cgccaggttg ccgctgacgc tcagcgtggg ggcgccggcc tgcgcgaacg cggcgcgcag
    51601 gtcggcgtcc agcgtggccg cctgcagccg cagcggcagc gccgccggca ggtaggccag
    51661 gtagggctgc aggtcgaacg acttcagctg caggctggct tcggtctggc gcgaggcatc
    51721 gaacggcagc gcctgcgcgc gcgagtcgaa ggcgctgcca ttgaccacga aggccagctg
    51781 cggctccacc ttgatctggc gcgccgccgg caggctgctc agaaacggca gcgccagccg
    51841 caactggcgc acctggtgct gccggccggc cgcgcggtcg tcgaagtcga tgcgcccgtc
    51901 ggtcagctcg atgttgtaga gcgcaaagcg cggcggcgcc gcgggttcgg gcgccgccgg
    51961 ccgcgccgcc agccgcgcca gcaggtcgtc gatgtcgtag cgcccctcgc ccaggtgcgt
    52021 caggtgcagc gcgggcgact cgatgcgcag ggcatcgatg acgggcgcca ggcgaaacac
    52081 cgattgcagc tcggcgtcgg cccacaggcg cgccacgctc agcagcggcg gcgcgcccgg
    52141 cgcggcgtcg atgcgcaggt catggagcgc cagctgcaac gaccagggcc gcaccaccac
    52201 cgcgccgatg tgcaccggcc gccccagctc ggcactggcg atcttctcgc cctgccactt
    52261 gagcagcggc ggcagcgcca gccaggccag cgcccacagc gccagcaggc cgaccagggc
    52321 gatggtcagg cggcggcgcc agcgccgggg gggccaccag gcccgctccg gcggggcggg
    52381 cgacgcggcg accgccgcgc gggtgggctc ggggggtggg gaagacagca gagaaccaga
    52441 catcgggcac ggattgtcgc cctcgacctg gcgccctcca cctgcgacat ggcaaccgcc
    52501 tgcaacaggc gcgcgcccat gagaaagccc ggcgtttcgg cctgcggccg gcccgccggg
    52561 ctttcaggca gcgtgacaaa agcgtcgtgg tctgccaggg tccgcatcgg aaagcttgct
    52621 gtttccggct gcggcgggaa gcaagagatt gcttcctttc aacggctgac ggcgggtcca
    52681 ttcgctgtat cggctacagc cgacggatgc ttgccaaccc atgcccgggt cacccgggcg
    52741 acttcactgt acccagccgg cgccgccaga ccaagcgcaa atattcaatc cgccggccca
    52801 cccgcattgc ccgcgccaat cggcgcgcgc agcccgccag atgccgccat cgcctgctcc
    52861 cacggacgga gcagccacca ggcgcatccg tttcttatat cgaaattaga atcaaaactc
    52921 cagatttcag ggttgaccag gtattttgag cactcctaga atcgtctttt ccgacaaagt
    52981 ttgtgggaaa ccctgagacg tctgcccgac cgggcagtcc cggtctgacg acacctcccg
    53041 tcccgcgcgc ctgctgtcac cggcgcacgt cagccgcttc aggacccgac caagagactg
    53101 acaccaccgc gcaccctcgt tgctcccttg tcctgcgcgc cccgtggcgc gccggcctgt
    53161 gacctggtgc cgtgtgtcgt gaatcaagga gaaccatgac agcgtccaca cgccttgccg
    53221 ctctttcaag cggcctcagg accttcgccg gtgacgtggc ggaaggcttt ttcgaaatca
    53281 cgcacaacgg tctggccctg gtgggcgtcg tggtggtgct ggccgtggcc ctgctcggcg
    53341 cccaccccga actgcgccag cagggcgagc agcagttgac cgagtggctg acggcgcgca
    53401 aggtcgccgc tctgggcatc acgcccgaac tgggcgccat cgagcgcgcc accgccgcca
    53461 acccgcgcga actgccgcgc gaacaggcca acctggcgct gtggatcagc aagaaatacc
    53521 gcgtcgcgcc cgagccggtg gcggcgctgg tggccgaggc ctatgaagcc ggccaggccg
    53581 ccaagctgga cccgaccctg atcctggccg tgatggcgat cgaatcgagc ttcaacccct
    53641 ttgcccaaag ctcggtcggc gcccaggggc tgatgcaggt gatgacgcag atccactctg
    53701 acaagtacga gcatttcggc ggtcagtacg cggccttcga ccccaagacc aacctgcgcg
    53761 tgggcgtgaa ggtgctgcag gaatacatcg gccgcaccgg ctcggtcgaa atgggcctga
    53821 agtggtatgt gggggcggcc aacctggcca gcgacggcgg ttacgccgac aaggtgctgg
    53881 ccgaacacgc ccgcctgtac caggtcgcca agggcaagcc gctgccggcg gcgctgcccg
    53941 gcctgcgcgc gcagaagccg gcccctgcca gcccgcccga accgacggcg ccggtcgagg
    54001 tcgccaccct gtcctgaagc cgtggccgtg gcggccctag gctaaactga cgcccgtgcg
    54061 caactggcga taggcgcgcg gcccacgggc cggcgtcgac gtgggccacc acggggaagc
    54121 gcaccgcccg ccgtgcgcgg caggcgcgtt ccgccgttcg cctgggccgc cccggtccga
    54181 caccgggagc gacgtcaccc caaggaccgc catgttcaac cgttccagcc acaccatcgc
    54241 ccacaccgac cccgagctct gggccgccat ctgcgccgag caccagcgcc aggaagacca
    54301 catcgagctg attgccagcg agaactacgc ctcgccggcg gtgctggccg ccgccggcac
    54361 gcagctgacc aacaagtacg ccgaaggcta tccgggccgc cgctactacg gtggctgcga
    54421 gcatgtcgac gtggccgagc agctggccat cgaccgcgtc aagcagctgt tcggcgccga
    54481 ggccgccaac gtgcagccgc actgcggcgc cagcgccaac attgccgcct tcatggcgtt
    54541 cctcaagccg ggcgacacca tcatgggcat gagcctggcc gaaggcggcc acctgaccca
    54601 cggcatgccg ctgaacatgt cgggcaaatg gttcaacgtg gtcagctacg gcctgaacgc
    54661 gcaggaagcc atcgactacg acgcgatgga aaaaaaggcg cacgaaaccc gccccaggct
    54721 gatcatcgcc ggcgccagcg cctacagcct ggagatcgac ttcgcccgct ttgcccgcgt
    54781 ggccaaggac gtgggcgcca tcttcatggt cgacatggcg cactacgccg gcctgatcgc
    54841 cggtggctgc taccccaacc cggtgccgca cgccgacgtg gtcaccagca ccacccacaa
    54901 gagcctgcgc ggcccgcgct cgggcttcat cctgatgaag gccgaacacg aaaaggccat
    54961 caacagcgcg gtgttccccg gcctgcaggg cggcccgctg atgcacatca tcgccgccaa
    55021 ggcggtggcc ttcaaggaag cgctgcagcc cgagttcaag caatacgcgg cccaggtgcg
    55081 caagaacgcc gccgtggtgg cccagacgct gaccgagcgc ggcctgcgca tcgtcagcgg
    55141 cggcacgc
//
LOCUS       DAOOOG010000002        54297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_402_length_54297_cov_27.086206, whole genome shotgun sequence.
ACCESSION   DAOOOG010000002 DAOOOG010000000
VERSION     DAOOOG010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 54297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54297
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_402_length_54297_cov_27.086206"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PK242_00220"
     CDS             <1..640
                     /locus_tag="PK242_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3394 domain-containing protein"
                     /protein_id="HOZ92483.1"
                     /translation="LRTVILPFIWIFNPQLLLLDVHGWGELVLVVSACTLAMLGFAAL
                     TMNWLRIRLTWWEFALLALAVVMLFRPGFFMDRLAPEHTRVPAAQVLEVARALPDQGT
                     LVLVIKGTTLEGEEVEKTVAVQLGAQGPDGRARLADAGLTLMTLGDAVQVGAVRFGSQ
                     ARKSGFEQGWDIAHVQVPTDRPSKNWFLLPGMLLMALVWGLQGRRMRRTAPA"
     gene            complement(718..1461)
                     /locus_tag="PK242_00225"
     CDS             complement(718..1461)
                     /locus_tag="PK242_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOZ92484.1"
                     /translation="MLPILYQDAQLVAVDKPAGLLVHRTGLDAGETRFAVQWLRQQIG
                     RPVWPVHRLDKGTSGVLLLALDASCAATLAQDWAGGGVRKTYVAMVRGWPPAAGLIDH
                     PLARLPDDARGARGLERQPALTRFRTLARLALPLPDRGFAQTRCARIELQPDTGRRHQ
                     LRRHCKHMAHPIIGDATHGKGALNRAVAALLGSQRLWLHAQSLVLRHPVSGAPLHIEA
                     APGPEWALWAPWTPTWCDAPRHAASRSDI"
     gene            1460..2233
                     /gene="yaaA"
                     /locus_tag="PK242_00230"
     CDS             1460..2233
                     /gene="yaaA"
                     /locus_tag="PK242_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOZ92485.1"
                     /translation="MLFLLSPAKALDYDTPLPADLPHTQPLFVPEATALIEVLRQQTP
                     AQIASLMKLSDALASLNVARYQAWRPRFGPRQARQAVLAFNGDVYEGLQARTLTASQL
                     DWLQQHLCILSGLYGVLRPLDGMRPYRLEMGTALATERGRNLYQFWGPRIADHLNQRL
                     AGQPAPIVVNLASQEYFKAVDRWVLQARVIDCVFEVRQGGAFKVVGVHAKRARGLMMR
                     YAAQHRIASPQRLQGFDLEGYAFAPAASTPDRLVFRRSA"
     gene            2242..3090
                     /locus_tag="PK242_00235"
     CDS             2242..3090
                     /locus_tag="PK242_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HOZ92486.1"
                     /translation="MSQTITPELRQWIVDQARAGHSADAVLQSMKSSGWNEDVAIEAM
                     ESTLRGHLDQQAQAQGLPPAVPVPEPDVADSPLYLDGGDRQVAVLGSLALPRVVILGG
                     LLSDQECDALIAAARPRLSRSLTVATQTGGSEVNPDRTSQGMFFQRGESELVARIEAR
                     IARLIRWPVENGEGIQVLHYLPGTEYKPHYDYFDPKEPGTATIVKRGGQRVGTVVMYL
                     SEPEKGGGTSFPDVFFEVAPKRGNAVFFSYERPHPSTKTLHGGSPVIEGEKWIATKWL
                     REREFR"
     gene            3144..4052
                     /locus_tag="PK242_00240"
     CDS             3144..4052
                     /locus_tag="PK242_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ92487.1"
                     /translation="MQIEANGIRIEVEDSGGDGPVVMLLMGLGGQLIHWPAAFVQALV
                     DAGWRVLRLDNRDAGLSSHFSAHGVPAIPWVAAQAWLGRRPRVPYTLGDMARDALGVL
                     DALGVARAHVVGLSMGAMIAQRLALLAPARLWSLTCISGCSGARGLMKPQASVLRVAA
                     GKPRAGDDAALAAYYLNFLRAIASPRYPPSDAELRDVLARTARRHAPHSDATTRQLAA
                     ILADGGRAGQLARIRCPTLVVHGADDPWVPPACAHDTARRIAGAQPALIPDMAHDLAP
                     AGHPEIVRRVLAVLLPFLPSAQVHTP"
     gene            4049..4906
                     /gene="queF"
                     /locus_tag="PK242_00245"
     CDS             4049..4906
                     /gene="queF"
                     /locus_tag="PK242_00245"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575038.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HOZ92488.1"
                     /translation="MTTPATPHDSPLGRASAYSDQYDPTLLFPIARAPQRAQLGIQGQ
                     PPFFGADWWTAYELSWLNARGKPQLAIAHIMVPCDSTHIIESKSFKLYLNSFNGTRID
                     RTEELVRRLRQDVSAAIWQGGPVQAAAGVRLIAPDDFDREAVHELDGVNLDRLDIDVD
                     GWDEPAPQCLSAAFDEQPVEEALTSRLLKSNCPVTGQPDWGSVQIRYAGPQIDQAGLL
                     RYLISFRRHNDFHEHCVERMFTDIWERCRPARLSVYARYTRRGGLDINPWRTSHPGPL
                     PPNVRTARQ"
     gene            complement(5102..5950)
                     /locus_tag="PK242_00250"
     CDS             complement(5102..5950)
                     /locus_tag="PK242_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOZ92489.1"
                     /translation="MNIPALTCFQLSVSDHIAHLVLSRPEAMNTMGPAFWRELDQALE
                     LLQRDGAARALVISSTGKHFSAGMALDVFGGAIQMDDAHPEGRAAIADLLADLQATFT
                     RLETLRIPVIVAIQGGCVGGAVDMVTAACIRYATQDAFFCVQEINIGMVADVGTLQRL
                     PKLVPLAVVKELAYTGRRMPAARAREVGLVNEVFDSADACLAAAMQCAREIAAKPPVA
                     IWGSKQVIHYARDHTVDDSLRQMGWVQAAIWSNAHVREAVTAMKQQRAGAFPPLATLT
                     RFTDHD"
     gene            complement(5947..6246)
                     /locus_tag="PK242_00255"
     CDS             complement(5947..6246)
                     /locus_tag="PK242_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92490.1"
                     /translation="MLTAWCLNELQRIKAWLVAHKPSHPLEYHSFDAVLTLWMMGWIG
                     WLPVLLFDEVLWAWPLCLLGMWAPALYVRWRARAHAAGRLRCDWLGALASTTVRV"
     gene            complement(6343..7557)
                     /gene="dinB"
                     /locus_tag="PK242_00260"
     CDS             complement(6343..7557)
                     /gene="dinB"
                     /locus_tag="PK242_00260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOZ92491.1"
                     /translation="MDAFYASVEWLRYPQLKGLPMVIGGGRRREDELIARLNRQRPER
                     VWTAADLAEIPVDCFPLLRDYVGRGVITTASYAARQFGIGSAMGLMKAARLCPQAILL
                     PADFDEYRRCSRAFKRVILDIAPVMEDRGIDEVYIDFTQVPGGQREGGRVLARLLQKS
                     IADATGLSCSIGVAPNKLLAKMASEFDKPGGISIVRDSDLQSLIWPLPCRKINGIGPK
                     TDARLQQHGIHRIGDLAARERAWLIAQFGASHGAWLHDAAWGRDERPVVTDSEPVSLS
                     RETTFERDLHAVHDRAELGAVFTRLAEAVAADLQRKGYAGKTIGIKLRFDDFRIATRD
                     QTVAEPTQDARRIRQIAGQCLKRVELSRRLRLLGVRVGSLVRADRLSAASDEASNHDL
                     PLFQQAPAAPEN"
     gene            complement(7658..8974)
                     /gene="fahA"
                     /locus_tag="PK242_00265"
     CDS             complement(7658..8974)
                     /gene="fahA"
                     /locus_tag="PK242_00265"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HOZ92492.1"
                     /translation="MSTLNDTHDPHLTSWVTSANDGRTDFPIQNLPFAIFRRRGGSEP
                     WRGGVAIGDQIVDLAAAAQAGLFHGEAQAAAQAGAEPSLNRLMSMGHQAASSLRKALS
                     KALQSGSPQQAALQPCLVPQAEAEYDLPCRIGDYTDFYTSVHHATNIGKQFRPDNPLL
                     PNYKWVPIGYHGRSSSIGVSGQAFHRPRGQVKPPDADAPSLKPSARMDIELELGVFIG
                     ASNALGAPVALDEAEAHVFGLVLFNDWSARDIQPWEYQPLGPFLSKNFASTISPWVVT
                     MEALAPFRTPFTRPADDPQPLPYLASPAHSAAGGLDIELEALIQTARMRAAGSPPTRI
                     THTNYRHAYWTAAQMVAHHTVNGCNLQPGDLLGTGTLSGPTLDQACALIELTTGGKHP
                     IALPNGETRSWLEDGDTIVLRGWCARPGAARIGFGECVGTVLPARD"
     gene            9441..11063
                     /locus_tag="PK242_00270"
     CDS             9441..11063
                     /locus_tag="PK242_00270"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ92493.1"
                     /translation="MASFEENHVLTLDSQAPQATENAASDNPFQALGLAPELLAAVAE
                     LGYSAPTDVQRQAIPLALQAHPEGANDLMVSSQTGSGKTAAFLLPVLHTLLDMQREAQ
                     AREQAAWDARVAEALARGEPAPRKPRRKNPLNPRQFKPAVPGALVLCPTRELAQQVAR
                     DAIDLVRHCRGLRIANVVGGMPYGQQIAQLQNAALVVATPGRLLDLQRSGQIVLDQVR
                     FLVVDEADRMLDLGFADALAEIHQLTAQRQQTMMFSATFAAPIQQLALRVMHDGGARV
                     RKVQVATPQQSHANIRQELYWADSPEHRRRLLDYWLRDPSIDQAIVFACTQIECDQLA
                     ADLQQSGFAAVALHGALSQALRNRRLRALREGKVQILVATDVAARGIDVPSITHVINH
                     GLPMKAEDYTHRIGRTGRAGRDGLAVTLAEFRDRRRLAEIEAYTRQPFRPLVVAGLEP
                     TRHFPPVSEPRAPRRPAAHDGGARRAGGPRGAARPTRDRQAEFSPAARLAGAGSYQNK
                     SSPGASARPRAAAGRARAGGAPRPYTPGRAGR"
     gene            11067..11666
                     /locus_tag="PK242_00275"
     CDS             11067..11666
                     /locus_tag="PK242_00275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ92494.1"
                     /translation="MGADASRWRIAPLAGADVPALLQVQAACYGPALLEDAAVYARRL
                     ASPAHCGLGLHAGGALAAYLAAHWSVWGAVTPLHGDFAPQAAADTLYLHDLAVRPEHA
                     GQGLGQRLLAAAWRQARARGVRQAALVSVQGTREFWQRQGFVVALRQPPALASYGSQA
                     LYMTRRLDDAAEPGSTGPINGRAPARAPGSSGPAGPAPA"
     gene            complement(11602..13029)
                     /locus_tag="PK242_00280"
     CDS             complement(11602..13029)
                     /locus_tag="PK242_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOZ92495.1"
                     /translation="MTTDTPLLDLLRQTVGAPHVLTEGDLTAYEQDWRRRVRGRALAV
                     VRPGSTAEVAAVVRACAAHGVAIVPQSGNTGLSVGSVPDDSGTQLLLNLGRLDRVRAI
                     DRANLTLTAEAGCVLQRLQAAAQDAGLLYPLSLGSEGSCMLGGNLGANAGGTQVLRYG
                     NTRELCLGLEVVTAQGEVWDGLSGLRKDNTGYDLRDLFIGSEGTLGIITAATMKLYPR
                     PAATLTAWAAVPSLAAALELLGLAQRHLGAGLTGFEVMGQFALGLVDRHLPQLRVPLW
                     RDTPWCVLLENADQESQAHARARFEPLLEAALDAGCVSDAVVAESIAQARALWHIRES
                     IPLAQAEEGLNIKHDIAVPVSRVPDFVAETEALLARQIPGVRLVVFGHLGDGNLHFNV
                     QAPEGGDAAQFLRQQEERVNLLVFDAVARHQGSISAEHGIGSLKAALLPRYKSPVALT
                     LMRAIKQALDPQGLMNPGRVLARDR"
     gene            complement(13090..13485)
                     /locus_tag="PK242_00285"
     CDS             complement(13090..13485)
                     /locus_tag="PK242_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HOZ92496.1"
                     /translation="MPAHPSPAVQATRVVAVASLLALMALGLAWELWLAPLRPGGSWL
                     ALKVLPLALALPGLLRYRMQTCRGLALFVWLYFVEGVVRATSDHGVSARCALAQVALV
                     LLLFAACAAQVRLRQRAARAAAAGPAVSH"
     gene            13508..14752
                     /locus_tag="PK242_00290"
     CDS             13508..14752
                     /locus_tag="PK242_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HOZ92497.1"
                     /translation="MQFDALAARAQAFLTELTDFPVRPTAHTLWQRFRQDRLGLTASA
                     LTFTTVLALVPFVAVALSVFTAFPAFGQLQQALQQWLIDSLIPASIADSVMGYLTQFA
                     GQASELGLLGFAGLTLVSLSLMLTIDKTLNQIWRSSQTRPLSQRILVYWTALTLGPLV
                     MGASLAISSYVVTASRGWLPDLPGSVDAALALLQLLLMVGSLTLLYRLVPNTPVRWRH
                     ALAGALFVSVCLGLARQGLGWYLTRMPTYSVIYGTLATLPILLLWIYLGWLIVLFGAV
                     IAAYLPSLLSGVARRGGGPGWDFRLALELLQALAAARDGAGRGLTLPQLAQALRVDAL
                     QLSAAMAALTGLDWVGQLGTRSENEEPRHVLLIEPRRQLLRPLVERLLLAPDEALAGW
                     WARGLRSEATVADVLSPAATDS"
     gene            complement(14795..15898)
                     /gene="mutY"
                     /locus_tag="PK242_00295"
     CDS             complement(14795..15898)
                     /gene="mutY"
                     /locus_tag="PK242_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOZ92498.1"
                     /translation="MNGGGAELAARVMAWQRRQGRHDLPWQGTRDPYRVWLSEIMLQQ
                     TQVTTVLAYYPRFLARFPDVERLAAASEDEVLGLWSGLGYYRRARHLHRCARDVVAHH
                     GGRFPASAAELARLPGIGRSTAAAIAAFCFGERAAILDGNVKRVLTRALGFGHDLALA
                     RHEQALWVQAEALLPRTDLRRAMPVYTQGLMDLGASVCHRRRPDCPRCPLHDLCVAAA
                     AGQPERYPVKRRRVARQRLELWLLLARDAQGRVWLQQRPEHGIWARLFCLPLFDDRAT
                     LLASLPARVHGQAVTEPPRRHVLTHRDLDLHPVSVRLRATDLRHADGRWFGADEWPAL
                     GLPAPVRRLLAAPAAAPQAPRGRLGEVRPSVQK"
     gene            complement(15895..17814)
                     /locus_tag="PK242_00300"
     CDS             complement(15895..17814)
                     /locus_tag="PK242_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOZ92499.1"
                     /translation="MSSRLKNLAAWLRQQRLLDDATDESLQRLVEQLRSDKVMVAFVA
                     EFSRGKSELINAVFFAGYGRRIMPASAGRTTMCPTELAYDAELPPCLRLLPIETRLQP
                     QALMEWRMAPEQWTRVDLDVNDPQQLASAMEKVSEVRYVDQAQARALGFWHDDRPEDN
                     PPVNAQGLVEVPRWRHALINMAHPLLRQGLVVLDTPGLNAIGAEPELTVNLIPQAQAV
                     VFILAADTGVTKSDLGIWREHVATMQDSSSSRFVVLNKIDALWDELSTPAQIEAQIAR
                     QCADTARTLALQDDQVLALSAQKGLLAKVRGDAALLADSRLPHFEDLLGQQVLGARHA
                     MLHDAIGAGVARAQAEAERTLAMRGRELSDQTIELNGLRGKNHQVIHQMRLRIEQEKA
                     DFDRSAASVLAVRSVHLKLLREVFQLLGNASIKRELAELNAALREPGLKLGIKKSYAD
                     GFERLRGVIRQVQALEGEIHAMLKAAFVQLNTDYGFALQAAEPPAMERFVSDLDAVER
                     SHLRYLGVGHMLQLARAGFSEKLVRALLTRVRSIFETALGDIELWNKATTSQLDTQLR
                     ERRRAYGRRIDAVERIQDAAGSLDQRIHDLQQEQQALQATAQRLRELGHELRRASATD
                     AADTAPPELTEVVPA"
     gene            complement(17965..18780)
                     /gene="mutM"
                     /locus_tag="PK242_00305"
     CDS             complement(17965..18780)
                     /gene="mutM"
                     /locus_tag="PK242_00305"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOZ92500.1"
                     /translation="MPELPEVEVTRLSFADRIRDARILAARPGLPLRWPLGCAPEQLE
                     DRTVQDVRRRGKYLLLHLDQGVLLLHLGMSGSLSFDAAPAAPGRHDHFDLVTTHGTLR
                     LHDPRRFGAVVWSASLQLDPARKLLASLGMEPLGERFDPAAFHAGLRGRRAPIKQVLL
                     AGDVVVGVGNIYASEALFQAGIRPTVRADRLSRARADRLHAAVRQVLARAVERGGSTL
                     RDFSSATGESGHFQLEAAVYGRAGQPCLQCATPVRLIRQGQRSTYFCPRCQRA"
     gene            18939..20588
                     /locus_tag="PK242_00310"
     CDS             18939..20588
                     /locus_tag="PK242_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ92501.1"
                     /translation="MPETSRLNARLLYELLLAELLAREGEAQQGAAYMLNAARRTGDE
                     ALFKRATELAIQSRAGPAALEATRAWRQAFPDSAQAGQYELQVLIVLGRVAETEEPTR
                     HFLATLPEAERLSFITALPALYQRVPDKAEAARVIERALDGALSQPALAPAAWTSIGR
                     LRLQAGDKAGALAAATLGQKADASSEWPALLGLQLMGSGETQAEALVERHLSTAQARP
                     ELFIAYARALIEQGRGGDAHRQLDRLIARAPEHPEAWLVRGALYADERKDAEAEADLR
                     RYLSLLEATPATPDAEPPEGSAHARMMLAGIAERRGELTQAQQWLASVDSPEQALAVQ
                     TRLAQILARQGRLEEARQSIRAAPERSPEDARLKLLAEAQLLRDHGQPGQAYELLNGA
                     LQQTPDEETLLYEAAMAAERVGRFEVMERLLRRLIELKPGASHAYNALGYALADRNLR
                     LPEARTLIEKAVQLSPNDAYIQDSLGWVQFRQGNLRQARQTLEAAFRKRPDAEIAAHL
                     GEVLWILGEREAALRVWREGQRLEADNETLRKTLLRLRVRL"
     gene            20633..21082
                     /locus_tag="PK242_00315"
     CDS             20633..21082
                     /locus_tag="PK242_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HOZ92502.1"
                     /translation="MIAGCAHQTGANAPFDSQTSHWSGRLGLTVASEPPQSYSAGFAL
                     SGNALVGELMLTSPLGSALAVMRWQPGQALLQQGEQTARFESLDALVEQVTGTPLPVR
                     ALFGWLHGQPQSVPGWQADLSGLPDGRLFAQRLMPLPTAELRLVLDR"
     gene            21097..21954
                     /gene="ispE"
                     /locus_tag="PK242_00320"
     CDS             21097..21954
                     /gene="ispE"
                     /locus_tag="PK242_00320"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOZ92503.1"
                     /translation="MRAFYDLPAPAKLNLFLHVTGRRADGYHLLQSAFMLIDWCDTLH
                     VERRAGGALSRQDLGPTLPADDLILRAARALQQASGCAEGAHIMVHKRIPAQAGLGGG
                     SSDAATTLLALNRLWRLGLNRAQLARIGLALGADVPFFLHGTHGWVEGIGERIRTLQL
                     PPARFVVVKPPQGLETAAIFRDPALKRDSKPAIIAGFAADPYGFGHNDLQPVAERLCP
                     QVGQGLALLAQHGLHGRMTGSGSAVFAPVVPGAMTGVTEVQVPPDWGLRWCSNLEFHP
                     LGGWAPGEV"
     gene            21994..22070
                     /locus_tag="PK242_00325"
     tRNA            21994..22070
                     /locus_tag="PK242_00325"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22028..22030,aa:Gln,seq:ttg)
     gene            22157..23134
                     /locus_tag="PK242_00330"
     CDS             22157..23134
                     /locus_tag="PK242_00330"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOZ92504.1"
                     /translation="MQPTDSSDFLVFTGNANPTLAAEIAYNLGISLGAADVGRFSDGE
                     VTVEIKQNVRARDVFVVQSTCAPTNDNLMELLIMVDALKRASAERISAVIPYFGYARQ
                     DRRPRSTRVPISAKVVANMLQAVGVNSVLTMDLHADQIQGFFDIPVDNIYASPVLLGD
                     LRQQNYDDLLVVSPDVGGVVRARALAKQLDCDLAIIDKRRPKANESEVMHVIGDIEGR
                     NCVVMDDMIDTAGTLVKAAEVLKQRGAKKVYAYCTHPIFSGPAIERIAKGSALDEVVV
                     TNTIPLSAAAAACPKIRQLSVAPLIAQTIQRIARGESVMRLFQEQENLF"
     gene            23229..23846
                     /locus_tag="PK242_00335"
     CDS             23229..23846
                     /locus_tag="PK242_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOZ92505.1"
                     /translation="MKFVAFERSKQGTGASRRLRNAGKVPGIVYGGEGEPQLIELDHN
                     ALWHAIKKEAFHSSVLDMELGGKTAKVLLRDVQVHPYKQLVLHVDFQRVSAKQKIHMK
                     VPLHYRGEEESNAVKLDKCLINHILNELEISCLPADLPEAIEVDLSKLEKGATVTLKD
                     IALPKGVEPVLRGHSAEDTVLVSVVTPAAEAAEGEAPAAAEGDKK"
     gene            23961..24608
                     /gene="pth"
                     /locus_tag="PK242_00340"
     CDS             23961..24608
                     /gene="pth"
                     /locus_tag="PK242_00340"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOZ92506.1"
                     /translation="MIKLFVGLGNPGPEYAATRHNAGFWWIDQVARQLGVRLVPERAF
                     HGLVARASVQGASVWLLQPQTFMNLSGKSVAALARFYKVAPQEILVAHDELDLPAGEA
                     KLKQGGGHAGHNGLRDIHAQLGSSDYWRLRLGIGHPGNRQEVVGWVLKKPPLDEQIGI
                     EQAIDRAARAFALLAAGDMTAATQAIHTSRPPRPKPPRKPPPSPADAGASERPDV"
     gene            25062..25706
                     /gene="lolA"
                     /locus_tag="PK242_00345"
     CDS             25062..25706
                     /gene="lolA"
                     /locus_tag="PK242_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HOZ92507.1"
                     /translation="MHVPRLSAVALVWFALGAPVARADGLQNLDAFIRHSRSGSADFT
                     QTVTAPAKPGQPARVKTSSGRFEFQRPSRFRFDYQKPFVQTIVADGKTLWLYDADLNQ
                     VTQRAQAQVLGQTPAALIAAAPDLRALQAEFTLAAAPDRDGLQWVSATPKQRDGQLQQ
                     VLVGFDGERLAALEIVDSLGQRSVLKFGRIEVNPALPASTFEFKPPAGADVLRQ"
     gene            complement(25729..26601)
                     /locus_tag="PK242_00350"
     CDS             complement(25729..26601)
                     /locus_tag="PK242_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOZ92508.1"
                     /translation="METTQDIKTSSLTSIQDAWATLQAHAQNGTPLEPAAYRLAFADP
                     EFLLRPETRGVRFQLELLKPDLDLSAAGVHSTVVVYGSARFVAPDEAAQQLASARTSG
                     DAAALAAAELAVRNAVYYAQAREFARLVATHSERQRAEDRLFICTGGGPGIMEAANRG
                     AHDVGMPSVGLNIALPHEQHTNAYVTPYLAFKFHYFALRKMHFMMRAKALVVFPGGFG
                     TLDELFEVLTLVQTRKARPVPIVLCGSSYWSRLLNLDMLVEEGAIAPEDLQLYRVVDT
                     PAQAWDAIQSFYRL"
     gene            complement(26870..27424)
                     /gene="efp"
                     /locus_tag="PK242_00355"
     CDS             complement(26870..27424)
                     /gene="efp"
                     /locus_tag="PK242_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOZ92509.1"
                     /translation="MKIAQEIRAGNVIMHGKDPMIVLRTEYARGGRGAATVRMKLKAL
                     LSNMGTEVVFKADDKMDQVILDHKECTYSYFADPMYVFMDGEYNQYEVEAGNMGDALS
                     YLEDGMAVEVVFYDGKAISVELPTSVEREITWTEPAVKGDTSGKVLKPAKIATGFEVA
                     VPLFVNQGDRIEIDTRTGEYRRRV"
     gene            complement(27559..28734)
                     /gene="earP"
                     /locus_tag="PK242_00360"
     CDS             complement(27559..28734)
                     /gene="earP"
                     /locus_tag="PK242_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HOZ92510.1"
                     /translation="MAPPRRALPHNASVPEPHPPLRWDIFCQVIDNHGDLGVCWRLAT
                     ALAAREQQVRLWVDDARALDWMAPAATRPTGVQVLPWTRPLDPMRLAGLPPADVWVEA
                     FGCQIATEFIAAGAERISAANHKGLKPPVWINLEYLSAEGYVERMHGLPSPVLQGPAR
                     GWTKWFFYPGFTPATGGLLREDDLPARQAAFDRRAWLAGQGIDWQGERLISLFCYEPP
                     ALATWIAQLRGSPEPTRLLVTAGRARQAVEQVLEQENRLQRPSSQRQQLSISYLPRFT
                     QIAYDELLWACDLNFVRGEDSLVRALWAGQPLVWHIYPQPEDNAHHAKLRAFLDWLEA
                     PASLRQFHLAWNGIDGGTLPAPDLPAWRRGVQAARQRLHAQTDLATQLLRFVARQTL"
     gene            28760..30736
                     /gene="uvrC"
                     /locus_tag="PK242_00365"
     CDS             28760..30736
                     /gene="uvrC"
                     /locus_tag="PK242_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOZ92511.1"
                     /translation="MTDAPAPDDAPPPPPAAPRHRPELLAQVAALPGLPGVYRYYDAA
                     DNVLYVGKARNLKRRVSSYFQKNHGGTRIGHMVGKIARLETTVVRSEAEALILENNLI
                     KQLAPRYNILFRDDKSYPYLKITGSEGVPDRGEGAPRPEAYARMTYYRGATDRRSRYF
                     GPYPSAWAVKETIELLQKVFRLRTCEDTVFAHRTRPCLLYQIKRCTAPCVRLIGQADY
                     AADVAQAERFLRGQTQQLLTEMERRMMAHADALEFEQAAAIRNQIQSLSRVLHQQAVE
                     VGGEADVDIIAVKVQGGRACVNLAMVRGGRHLGDRAHFPSQVEDAHALAGEREDAELT
                     SVEAQVLDAFIAQHYLALPTPPQLITSEPVTRTLLQALSDKEERRISATHAPRGQRRV
                     WLEMAQQNAALQLARLLAEEGSQQARTRALAEALELPTEALDQLRIECFDISHTAGEA
                     TQASCVVFEGHRMASSEYRRYKIDGITPGDDYAAMRQVLQRRYGKIAEALREQGNAAR
                     VRMPDLVLVDGGKGQVSVAREVFEALALDLSLIVGVEKGEGRKVGLEELVFADGRPKV
                     YLGHDSAALMLVAQIRDEAHRFAITGMRAQRAKVRVGGSQLEDIPGVGPRKRARLLQR
                     FGGVRGVEAASIDDLATVEGISRALAEDIYRALH"
     gene            30864..31430
                     /gene="pgsA"
                     /locus_tag="PK242_00370"
     CDS             30864..31430
                     /gene="pgsA"
                     /locus_tag="PK242_00370"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOZ92512.1"
                     /translation="MFFTLPTLLTWTRIVAIPLIVGVFYLPLAPHTANLVATVMFMVF
                     AWTDWLDGFLARKLNQTSAFGAFLDPVADKFLVCASLLVLVHLQRTDVFVALIIIGRE
                     IAISALREWMAKIGASKSVAVHMVGKLKTAVQMVAIPFLLYDGVLLERIDARWWGNLL
                     IWVAAVLTVWSMVYYLRKALPEIRARVQ"
     gene            31438..32355
                     /locus_tag="PK242_00375"
     CDS             31438..32355
                     /locus_tag="PK242_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ92513.1"
                     /translation="MNPSSPDAGGATLIRLMPAVFVVIWATGFIVARYGMPHAPPFSF
                     LLLRYAFSVACFLPWIVLARVAWPRTGLEWLHLGVTGVLMHGGYLGGVWAAVKGGMGS
                     GLSALIVGIQPVLTAVWLAGMGGAGARVSARQWSGLLLGFAGLVLVVWRKLTHGAPGD
                     HVTATNLAWAVLALVSITAGTLYQKRFVRPCDVRTANTVQLLAAALVTAPLAWLEPES
                     VRWNGQVVGALAWSVLGLTLGGSSLLYLLIQRGAASSVASLMYLVPPTTAVMAWALFG
                     EPIGVTTLAGIALTAFGVSLVVRPAAGRP"
     gene            32667..32939
                     /locus_tag="PK242_00380"
     CDS             32667..32939
                     /locus_tag="PK242_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOZ92514.1"
                     /translation="MNKTELIEHIAKNADISKAAATRALEAAIGAVKTTLRKGGTVSL
                     VGFGTFAVGKRAARSGRNPRTGATIKIKAAKVPKFRPGKALKDALN"
     gene            32959..33034
                     /locus_tag="PK242_00385"
     tRNA            32959..33034
                     /locus_tag="PK242_00385"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32992..32994,aa:Val,seq:tac)
     gene            33119..35041
                     /locus_tag="PK242_00390"
     CDS             33119..35041
                     /locus_tag="PK242_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOZ92515.1"
                     /translation="MFDFVRRHTKLIMGVLFLLIIPSFVLFGIEGYTRFNDGATKVAT
                     VNGQPVTQMEWDSAHRLEIDRLRQGNPNLDLSLLDSPALKYATLERLVHERVLAAAAA
                     DEHLIATDARLAAELQRDPTIASLRRADGTLDMERYQQLLAAQGLTPAGFEANARHEL
                     SSRQVLGGVSETGFSSAAQAQVAMGAFQERREARVQRFAPAEFADKVQPTDADLQSYH
                     QTHASRYQAPETASVEYLVLDLESLKKSVSVSEQDLRTYYEQNAASFGTLETRRASHI
                     LLSAARDAPAAEREKAKAAATALLAQLRQAPDSFAELARKSSQDDASAASGGDLGRFE
                     RNKGMDPAIAQAAFGLAKVGDMSEVVESDFGFHIVRLTEIKAAEVPPFENMRAQLEDQ
                     LRTRLAQQQFSEMADEFRNLVYEQSDSLKPAADKLHLTIQTADKLSPTPAPGASGPLA
                     NPKFLGALFAADAVDKKRNTEAIDLGGNQIASGRIVSHTPAHARAFDEVRTEVRQAFV
                     RERGAELARQEGQARLKAWTAQPASATSLGSALVLSRDEPQGQPMALVEAVLRADPAK
                     LPGFVGVDLGAEGFAVARVDKVLAAPTQSAEQTAQSRARYDQLWGLAEARSYYELLKA
                     KYKAQILVPAPAPTAR"
     gene            complement(35449..35524)
                     /locus_tag="PK242_00395"
     tRNA            complement(35449..35524)
                     /locus_tag="PK242_00395"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35489..35491),aa:His,seq:gtg)
     gene            35649..36560
                     /locus_tag="PK242_00400"
     CDS             35649..36560
                     /locus_tag="PK242_00400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOZ92516.1"
                     /translation="MLKSDHLNDAQALLNLNDLPAAPSRRRALRLGVGMGYAAAALPV
                     MAQTAIRTPTDGLTVGEVNIDVAGFSMPAFRAQPAGRSGLPVVLVLSEIFGVHEYIAD
                     TAVRLARAGYLAIAPELFVRQGDAQSYGEMAKLMSEVIAKVPDAQVMGDLDATVAWAA
                     QHGGNSDKLAVTGFCWGGRQTWLFAAHHPKVKAGVAWYGRLVGTATPLTPRHPVDVAA
                     QLKAPVLGLYGGADTGIPLDTVEQMKAALAAAGTQGNAAARGSAFVVYPDAPHAFHAD
                     YRPSFRKEAAEDGWKRALAWFKQHGVA"
     gene            complement(36899..37759)
                     /locus_tag="PK242_00405"
     CDS             complement(36899..37759)
                     /locus_tag="PK242_00405"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOZ92517.1"
                     /translation="MTYTHLITVAELQALQASGAPLMVFDCSGELGNPARADAMFAEQ
                     HIAGSRQAHLERDLSAHPPAEAINGGRHPLPPRERFAAWLSAVGFANPMQAVVYDRNG
                     CNYCGRLWWMFKWAGHDAVAVLDGGLQAWLAAGGAVASGPAAEHPATRFQLQPPRRRL
                     LHVHEVQAGLGQPGQTVIDARAAARFRGDEEPLDPVAGHIPGALNRPYVDNLDGRGCF
                     KAPAQLRAEFDALLAGRDPAGVVHHCGSGVSAVPNLLAMELAGYAPAALYAGSWSEWC
                     RTPGLPVARG"
     gene            complement(37774..38661)
                     /locus_tag="PK242_00410"
     CDS             complement(37774..38661)
                     /locus_tag="PK242_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ92518.1"
                     /translation="MQAAWMVAASLFFALMSVCIKFASPYFGPLEIVCYRGAIGVLFM
                     WALCRSRGVRLHTSVPMMHVWRNVVGVSALVCWFYAIAHLPLATAMTLNYMSGVWVAA
                     FLVGGTLLMGQLADIRRQGPLVMAVMAGFAGVVMMLRPTLEQNQLFAGLIGLLSGLMS
                     ALAYIQVAALGRVGEPEARTVFYFSLGAALAGGIGMQFFDTHPLHTVQALWLLPVGIL
                     AALGQLCMTRAYTRGATMVVANLQYSGIVFAALFGLLLFGDQLPLIGWLGMATIVASG
                     IAATVLRARALPNPPAESH"
     gene            complement(38782..39918)
                     /locus_tag="PK242_00415"
     CDS             complement(38782..39918)
                     /locus_tag="PK242_00415"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HOZ92519.1"
                     /translation="MSDLSLQLQQAASQLPVHSYFDPALYEREKKLLFESGPRYVGHA
                     LAVPEAHDYFVLPQEAGGRALLRDAQGQIQLVSNCCRHRQAIMLGGRGNLLAEGKGHA
                     GGNIVCPIHRWTYSSRGELLGAPHFERDPCLNLSNYPLRQWNGLLFDDNGRDIAADLA
                     GMTTAKDLSFDGYVLDHVELHECNYNWKTFIEVYLEDYHVGPFHPGLGNFVTCDDLKW
                     QFGGEYSVQTVGLAPSFGKPGSDVYRQWHEVLLKYREGKLPERGAIWLTYYPHIMVEW
                     YPHVLTVSTLHPTGVDRTLNMVEFYYPEEIACFEREFVEAQRAAYMETAIEDDEIAER
                     MDAGRKALMQRGDNEVGPYQSPMEDGMQHFHEWYRRAMGMRVTT"
     gene            40159..40398
                     /gene="xseB"
                     /locus_tag="PK242_00420"
     CDS             40159..40398
                     /gene="xseB"
                     /locus_tag="PK242_00420"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOZ92520.1"
                     /translation="MSRKAASAPAQPASYGEAVQQLEQLIAGLESGQLPLEDLLSHYQ
                     RGTELLRYCRDRLQAVEDQVRVLDERGDVKPWMPE"
     gene            40395..41291
                     /locus_tag="PK242_00425"
     CDS             40395..41291
                     /locus_tag="PK242_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOZ92521.1"
                     /translation="MNARDFPTWSDRLLTRVEQALVDWVPVDAPAGLGEAMRYAVLDG
                     GKRLRPLLVLAAAEAVQGHAAAALRAACAVELIHAYSLVHDDMPCMDNDVLRRGKPTV
                     HVRFGQAQALLAGDALQALAFELLTPEGDEVPASMQAALCGLLARAAGATGMAGGQAI
                     DLDSVGRVLTEAQLQAMHRLKTGALLQAAVHMGAACATPALPAHTRAALDQYGAALGL
                     AFQVVDDILDVTADSAQLGKTAGKDAAADKPTYVSLLGLTQAQALAAQLHTQAHQALD
                     ASGLTALAPLRWLADRVVKREN"
     gene            41294..43198
                     /gene="dxs"
                     /locus_tag="PK242_00430"
     CDS             41294..43198
                     /gene="dxs"
                     /locus_tag="PK242_00430"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOZ92522.1"
                     /translation="MSQALLESIHSPADLRRLPRAQLKGLADELRACVLTNVARTGGH
                     LSSNLGTVELTIALHYVFQTPHDRLVWDVGHQTYPHKILTGRRERMPTLRQLGGMSGF
                     PRRDESEYDTFGTAHSSTSISAALGMALAARQKGEDRHAVAIIGDGAMTAGMAFEALN
                     NAGVERDARLLVVLNDNDMSISPPVGALNRYLAQLMSGHFYAAARSVGKTVLGTVPPL
                     FELARRLEQGAKGMVVPATLFEKFGFNYIGPIDGHDLDSLIPTLENIRDLMRDKAGPQ
                     FLHVVTKKGQGYKLAEADPVAYHGPGKFDPAVGLVKPSAPAAPTFTQVFGQWLCDMAA
                     HDRKLVGITPAMREGSGMVEFHKRFPDRYYDVGIAEQHAVTFAAGLACEGLKPVVAIY
                     STFLQRAYDQLIHDVAIQNLPVVFALDRAGLVGADGATHAGNYDIAFLRCVPNVSLAC
                     PADERECRQLLTTAYEQSHPVAVRYPRGAGAGVAPLAQLTGLPFGKGEVRRQGKGVAI
                     LAFGTLLYPALAAAERLDATVVNMRWVKPLDAELLLQVAASHDALVTIEEGAIMGGAG
                     SAVAEALAAAGVVKPLLHLGLPDRFIEHGDPARLLALEGLDATGIEGSVRSHLGSWLM
                     PPQRNLKAVS"
     gene            43314..44396
                     /gene="dctP"
                     /locus_tag="PK242_00435"
     CDS             43314..44396
                     /gene="dctP"
                     /locus_tag="PK242_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOZ92523.1"
                     /translation="MDRRSLIKRAGIAGVLAAGVAPAVHAQAAVRWRIATSFPKSLET
                     LHGCAVKFSETVKALSGGKFEVSVHAAGELVPAFGIVDALADDSLEMGETASYYYTGK
                     HPAFAFGCAVPFGFTAQQMQAWKRHGNGRKLMDELYDKYNFFGLSALSTGTQMGGWYR
                     KEMKTPADFKGLKMRLGGGVFGESMAKLGVVAQNMPVGEVYQALEKGTLDAVEFVGPY
                     DDEKLGFNKVAKYYYYGGWWEGSAELEFFINKKAFAKLSPENQAIVRAAADVAYADGT
                     SKYMHLNPISLKRLVGSGTQLKRFPKPVVEAGFKAAQAVYQEHSGKDALFKKIYEDMR
                     AFQRDQILWNRISELAFTQDMSTLKI"
     gene            complement(44485..46347)
                     /locus_tag="PK242_00440"
     CDS             complement(44485..46347)
                     /locus_tag="PK242_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOZ92524.1"
                     /translation="MEFIAANFAPVMFAGLIVFLILGFPVAFSLGAAGLFFALVGVEL
                     GVFTSAIYSWLPQRLIGIMSNETLLAVPFFTLMGLILERSGMAEDLLETVGQVFGPLR
                     GGLALAVVLVGAMLAATTGVVAASVISMGLISMPIMLRYGYDRRLASGVIAASGTLAQ
                     IIPPSLVLIVLADQMGKSVGDMYKGAFIPGFALMGLYILWVFALAIFKPSMVPALPPE
                     ARTFREPNGHSGYRSLGVLALLSTAVAVVLARNMEAVHTWWQGTPVEFVPTDEKIVVA
                     MCGGTFVAWLIALVNRITRMGLLSQLAERVTFVLIPPLLLIFLVLGTIFLGVATPTEG
                     GAMGAMGALIMAAMRRRINMDLLKQALGTTTKLSSFVMFILIGATIFSMVFQAADGPV
                     WVEHLMAKLPGGQIGFLIFVNVMIFLLAFFLDFFELSFIVVPLLAPIADKMGIDLIWF
                     GVLLAVNMQTSFMHPPFGFALFFLRSVAPEKPYVDHVTGKTIEPVTTMQIYKGAIPFL
                     VLQLTMVAILIAFPGLVTSNLDKKVDVDMKAVGEQMFEQLNQDSEAEGLGEGREDGIG
                     TQEPGSSSEESPAIEGLDAPADPSSPEAGQEGNQGQEDDDPVKALERAAGKPSS"
     gene            complement(46371..46967)
                     /locus_tag="PK242_00445"
     CDS             complement(46371..46967)
                     /locus_tag="PK242_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOZ92525.1"
                     /translation="MQALLKFSRAVDWLNASLGRFVIWLIFGATIISALNAVVRKAFD
                     TSSNAFLEVQWYLFAWSFLAAAGYTLLHREHVRIDVLNSRLPKKVQLWIEILGYVLFL
                     TPVCVIVLWMSTPIVFERIASGEMSPNPGGLIRWPVWAALPIGFVLLLLQGWSELIKC
                     IAFLRGQGPDPTVKALDKTAEQELADALRQQQSASQTH"
     gene            47123..47863
                     /locus_tag="PK242_00450"
     CDS             47123..47863
                     /locus_tag="PK242_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ92526.1"
                     /translation="MSTLAESARAALQLIASLDPLLWSVVGRSLAVSGLACLLACGVG
                     LLAGASLAVARFRGREAVLTLLHTLLALPSVVVGLLVYLLLSRSGPLGFLGWLFSFQA
                     MVLAQAVLVVPVIAALTRQTVEDADLRHGEQLASLGAGPCLRGLLLAWDERCALLMVL
                     MAAFGRAVSEVGAVMIVGGNIDGFTRVMTTAIALETSKGDLPLALGLGMVLLSVVLLL
                     NAAMAGLRHWRARTDALAPGAVQGRWAA"
     gene            47860..48603
                     /locus_tag="PK242_00455"
     CDS             47860..48603
                     /locus_tag="PK242_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ92527.1"
                     /translation="MSRPAGPPGAAAPLIRLQDVGVRFGRVQALQGVGLEIAAGECVA
                     LIGGNGSGKSTLLRVIHGLIAPTEGQLVVPARRAQAMLFQRPWMMRTTVQRNVALGLW
                     LRGLPWRQAQERALGALERVQLAELASRRAPGLSGGQQQRVALARAWAQQAQLLLLDE
                     PTASLDPHARLEVEALMRAFTGTAPDAAPLTMVFASHHLGQVRRLAQRVICLDQGRVL
                     ADLPVAQFFDHARLSSVAPVAAAFVQGEM"
     gene            48603..49430
                     /locus_tag="PK242_00460"
     CDS             48603..49430
                     /locus_tag="PK242_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOZ92528.1"
                     /translation="MQKFRNFWPLALLQTALAAILTIAFPVASAQTIVMASTTSTEQS
                     GLFAHLLPQFRQASGIDVKVVAVGTGQALDIARRGDADVLFVHDPKAEEQFVTDGWGL
                     KRHPVMYNDFVLVGPRSDPAGARGRDIVQAMRQLAGAHAPFISRGDKSGTHSAELRFW
                     AQAGLAEARGRGYRECGCGMGPALNMAAASGAYVLADRGTWLNFRNRADLAILVEGDP
                     RLFNPYGVLVVNPARHPQVKAAEAQKFVDWVTSPAGQAAIASYRIGGQALFFPDARP"
     gene            49517..50716
                     /locus_tag="PK242_00465"
     CDS             49517..50716
                     /locus_tag="PK242_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOZ92529.1"
                     /translation="MACFPSWRTLCHHALGVALGLLLLPAQAQIRVSINPGDQIEADR
                     VGIFNAWKGALDAGLRKAGASAAQARFSSDATADMNAGRANVFDLMVGPAPVIGSAVR
                     YGYVPVVGATQKARAVLVAAEGSAIADWAQSKGKRLGMPGQDSVVTYLLRGEVQAGNT
                     TLARQYPSIYQTRYQDALLTCLELRRCDVVAVEEVVAKRWAASGKKVKVVWQSREVPG
                     LSVALHKDAQVSADTLRSALMAELARLDAFGYTGTLTVKDFEYVSTLGYFTPRTLEGA
                     TLIEDPAQVESLLAAGARYIDARNQEEFAAGHVPGAILVPYVEKSAKDPGYDPKLDQF
                     DLQRLGDNKAQPLVFGCNGPECWKSFKASRAALQAGYTKVYWFRTGVPAWRSSGRPLD
                     RGDAKAG"
     gene            complement(50725..51831)
                     /gene="modC"
                     /locus_tag="PK242_00470"
     CDS             complement(50725..51831)
                     /gene="modC"
                     /locus_tag="PK242_00470"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HOZ92530.1"
                     /translation="MTQGTGHDIELRAQVRMGSGFRLGVELRLPGQGVTALFGPSGCG
                     KTTLLRAVAGLTRPSPGRVAVGGDVWQDDAHRIWRPTHRRPLGLVFQEASLFEHLTVQ
                     GNLDFGLQRVPAAERRVALPQAIELLGLGPMLARRPAQLSGGERQRVAIARALATSPR
                     LLLMDEPLASLDAARKAELLPYFERLTRELRLPMLYVTHALDEVIRLADHLVLLRDGE
                     VVAHGPVEALLTRPDVARTQGDAASALVSAQVLSFDPHYQLLQVAFDHGVLHCVHSAG
                     TPPRTPGQRVRLRVQARDVSLCLSAAHDTSILNVLPARVRSLAEDGPAQVLVALDLGA
                     TPLLARVTRKSAEALALAPGQPVYAQIKGVAVLD"
     gene            complement(51828..52514)
                     /gene="modB"
                     /locus_tag="PK242_00475"
     CDS             complement(51828..52514)
                     /gene="modB"
                     /locus_tag="PK242_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOZ92531.1"
                     /translation="MLDATSLAAVRLSIELALATTCILLVLGTPLAWWLARGDAGALA
                     WLRTPVRTLVAMPLVLPPSVLGFYLLVMLGPQGPLGRLTQALGWGTLAFSFWGLLLGS
                     LVYSLPFVVQPLVNAFEAIGRRPLEAAATLRAGPLDAFLHVALPLARPGYLTGAVMGF
                     AHTLGEFGVVLMIGGNIPGQTRVISVQIYDHVEALEYTQAHWLAGGMLVFSFLVLLTL
                     HLTQSRKAPA"
     gene            complement(52624..53379)
                     /gene="modA"
                     /locus_tag="PK242_00480"
     CDS             complement(52624..53379)
                     /gene="modA"
                     /locus_tag="PK242_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOZ92532.1"
                     /translation="MRPIRTLVTLAAVMVASWSAHAETVHVAVAANFTEPAKALAAIL
                     EKTTGHEARLSFGASGAFYTQIKNGAPFDVFLSADDERPARLEKEGDTVPGSRFTYAT
                     GQLVLWSAKPGLVDDKGEVLQGGQFNKLAIANPKNAPYGAAAVEAMNRLGLQATLQPK
                     LVTGESIGQTYNFVATGNADLGFVAMSQVLQGGRLKGGSMWVVPGSLHAPIVQDAVIL
                     KRGQGNPAAKAWMALLSSPRTKDLIRSYGYAVK"
     gene            complement(53451..>54297)
                     /locus_tag="PK242_00485"
     CDS             complement(53451..>54297)
                     /locus_tag="PK242_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOZ92533.1"
                     /translation="PRNAPSLVNAAYQATLDWSNPAPRTLEQQMHTPLFGEQPVEMGV
                     NDTNRDAIVQRLKDDARYPPRFAQAFGGEAMPIHWDNVIKAIAAFQRTLVAAGSRYDQ
                     ALAGTATLTESEQRGQRLFFGERALCAQCHSGPNLGGGAFVSAQGRVGEPGFYNIGLF
                     NIGGTGAYPTNNRGLLEFTGRPEDMGRFRAPSLRNVALTAPYLHDGSVATLEAVVALH
                     AAGGRVLGNGPYEGDGRANPYKDPRIDQIDIDAQDQADLVAFLRALTDDRMLARPEFA
                     DPFVP"
BASE COUNT         8240 a        18626 c        19242 g         8189 t
ORIGIN      
        1 gctgcgcacc gtgatcctgc ccttcatctg gatcttcaat ccgcagctgc tgctgctcga
       61 cgtgcatggc tggggcgagc tggtgctggt ggtgtcggcc tgcacgctgg ccatgctggg
      121 ctttgccgcg ctcaccatga actggctgcg catccgcctg acctggtggg aattcgcgct
      181 gctggcgctg gccgtggtga tgctgttccg gccgggtttc ttcatggacc ggctggcgcc
      241 ggaacacacc cgtgtcccgg cggcccaggt gctggaggtg gcgcgcgcgc tgcccgacca
      301 aggcacgctg gtgctggtca tcaagggcac cacgctggaa ggcgaggagg tcgagaagac
      361 cgtggcggtg caactgggcg cgcaaggtcc ggacggtcgc gcgcgcctgg ccgacgccgg
      421 cctgacgctg atgacgctgg gcgacgcggt gcaggtgggg gcggtgcgct tcggctcgca
      481 ggcgcgcaaa tcgggcttcg aacagggctg ggacatcgcc cacgtgcagg tgccgaccga
      541 ccgcccgagc aagaactggt tcctgctgcc gggcatgctg ctgatggccc tggtctgggg
      601 cctgcagggc cggcgcatgc ggcgcaccgc gcccgcctga tacgaactcg ctttcaacat
      661 gacccatggg caatgacgga tcaccttgct gcgcctcgat gacccatcgg tcatgggtca
      721 tatgtccgaa cgcgatgccg catgacgcgg cgcatcacac catgtcggcg tccagggtgc
      781 ccacagcgcc cattcggggc cgggcgccgc ttcgatgtgc agcggcgcgc cgctgaccgg
      841 atggcgcagc accagcgact gcgcgtgcag ccacagacgc tgcgagccca gcagtgccgc
      901 gaccgcccgg ttcagcgcac ccttgccatg cgtggcgtcg ccgatgatgg ggtgcgccat
      961 gtgcttgcag tggcggcgca gctggtggcg gcggccggtg tcgggctgca gctcgatgcg
     1021 ggcgcagcgc gtctgggcaa agccacggtc gggcagcggc agcgccagcc gcgccagggt
     1081 gcgaaaacgg gtcagggccg gctgccgttc caggccgcgc gcgccgcggg cgtcgtccgg
     1141 caggcgcgcc agcgggtggt cgatcaggcc cgctgcgggc ggccagccgc gcaccatggc
     1201 cacataggtc ttgcgcacgc cgccgccggc ccagtcctgc gccagggtgg cggcgcagct
     1261 ggcgtcgagc gcgagcagca gcaccccgct ggtgcccttg tccagccggt gcaccggcca
     1321 caccggccgg ccgatctgct ggcgcagcca ttgcacggca aagcgggttt caccggcgtc
     1381 caggccggtg cggtgcacca gcaggccggc gggcttgtcg accgcgacca gctgcgcgtc
     1441 ctggtacaaa atcggcagca tgttgttcct attgtctccc gccaaggcgc tcgactacga
     1501 cacgccgctg ccggccgatc tgccgcacac gcagccgctg ttcgtgccgg aagctacggc
     1561 actgatcgag gtgctgcgcc agcagacgcc ggcacagatc gcgtccctca tgaagctgag
     1621 cgatgcgctg gccagcctga acgtggcgcg ctaccaggcc tggcgcccgc gcttcggccc
     1681 gcgccaggcg cgccaggccg tgctggcctt caacggcgac gtctacgaag ggctgcaggc
     1741 gcgcacgctg accgcctccc agctggactg gctgcagcag cacctgtgca tcctgagcgg
     1801 cctgtacggc gtgttgcgcc cgctggacgg catgcggccc taccggctgg agatgggcac
     1861 cgccctggcc accgagcgcg ggcgcaacct gtaccagttc tggggcccgc gcatcgccga
     1921 ccacctgaac cagcggctgg ccggtcagcc ggcgccgatc gtcgtcaacc tggcgtcgca
     1981 ggagtacttc aaggcggtcg atcgctgggt gctgcaggcg cgcgtgatcg attgcgtgtt
     2041 cgaggtgcgc cagggcggcg ccttcaaggt cgtcggtgtg cacgccaagc gtgcgcgcgg
     2101 gctgatgatg cgctatgcgg cgcagcaccg gatcgcctcg ccgcagcggc tgcagggctt
     2161 cgacctggaa ggctatgcgt tcgcgccggc ggcgtccacg cccgaccggc tggtgtttcg
     2221 ccgctccgca tgaggttgcc catgtcccag accatcaccc ccgagttgcg ccagtggatc
     2281 gtcgatcagg cgcgtgccgg ccacagcgcc gatgcggtgt tgcagtcgat gaagagctcg
     2341 ggctggaacg aggacgtggc catcgaggcc atggaaagca cgctgcgcgg ccacctggac
     2401 cagcaggcgc aggcgcaggg cctgccgccg gcggtgccgg tgcccgagcc cgacgtggcc
     2461 gattcgccgc tctacctgga cggcggcgac cgccaggtgg cggtgctggg ttcgctggcg
     2521 ctgccgcgcg tggtgatcct gggcggcctg ctgtcggacc aggaatgcga tgccctgatt
     2581 gccgccgcgc gcccgcgcct gagccgttcg ctgacggtgg ccacccagac cggcggctcc
     2641 gaggtcaacc ccgaccgcac cagccagggc atgttctttc agcgcggcga aagcgagctg
     2701 gtggcgcgca tcgaggcgcg catcgcgcgg ctgatccgct ggccggtgga aaacggcgaa
     2761 ggcatccagg tgctgcacta cctgccgggc accgaataca agccgcacta cgactacttc
     2821 gaccccaagg agcccggcac cgccaccatc gtcaagcgcg gcggccagcg cgttggcacg
     2881 gtggtgatgt acctgagcga gcccgagaag ggcggtggca ccagcttccc ggacgtgttc
     2941 ttcgaggtgg cgcccaagcg cggcaacgcg gtgttcttca gctacgagcg gccgcacccg
     3001 tcgaccaaga cgctgcacgg cggctcgccg gtcatcgagg gcgagaagtg gatcgccacc
     3061 aagtggctgc gcgagcgcga gttccgttga tcggttgagc atcaaatcgg cctcccgcgc
     3121 gtacccagca agcgccagca gctatgcaaa tcgaagcaaa cgggatccgc atcgaggtcg
     3181 aggactcggg cggcgacggc cccgtggtga tgctgctcat gggcctgggc gggcagctca
     3241 tccactggcc ggcggcgttc gtgcaggccc tggtcgatgc cggctggcgc gtgctgcgcc
     3301 tggacaaccg cgacgccgga ctgtccagcc acttcagcgc ccacggcgtg cccgccatcc
     3361 cctgggtggc ggcgcaggcc tggctggggc ggcgcccgcg cgtgccctac accctgggcg
     3421 acatggcgcg tgacgcgctg ggcgtgctcg atgccctggg cgtggcgcgg gcccacgtgg
     3481 tgggcctgag catgggtgcc atgatcgccc agcgcctggc gctgctggcg cccgcgcgcc
     3541 tgtggagcct gacctgcatc agcggctgca gcggtgcgcg cggcctgatg aagccgcagg
     3601 ccagcgtgct gcgcgtggcg gcgggcaaac cgcgcgccgg cgacgacgcc gccctggcgg
     3661 cctattacct gaacttcctg cgcgccatcg ccagcccccg ctacccgccc agcgacgccg
     3721 aactgcgcga cgtgctggcc cgcaccgcgc ggcgccacgc gccccacagc gacgccacca
     3781 cgcgccagct ggcggccatc ctggccgacg gcgggcgcgc cggccagctg gcgcgcatcc
     3841 gctgccccac cctggtggtg cacggcgccg acgacccctg ggtgccgccg gcctgcgccc
     3901 acgacaccgc gcgccgcatc gccggtgccc agccggcgct gatccccgac atggcgcacg
     3961 atctggcgcc cgccggccac ccggaaatcg tgcggcgcgt gctggccgtg ctgctgccgt
     4021 ttctgccatc cgcccaggtt cacaccccat gaccactccc gccaccccgc acgattcacc
     4081 gctgggccgc gccagcgcct acagcgatca gtacgacccg acgctgctgt ttccgatcgc
     4141 acgcgcgccg cagcgggcgc agctcggcat ccagggccag ccgccgttct tcggcgccga
     4201 ctggtggacg gcctacgagc tgtcctggct gaatgcgcgc ggcaagccgc agctggccat
     4261 cgcccacatc atggtgccgt gcgacagcac ccacatcatc gagagcaagt cgttcaagct
     4321 gtacctgaac agcttcaacg gcacgcgcat cgaccgcacc gaggagctgg tgcggcgcct
     4381 gcgccaggac gtcagcgccg ccatctggca gggcggcccg gtgcaggcgg cggcgggcgt
     4441 gcggctgatc gcgccggacg acttcgaccg cgaggcggtg cacgagctcg acggcgtcaa
     4501 cctggaccgt ctggacatcg acgtcgacgg ctgggacgag ccggcgccac agtgcctgag
     4561 cgcggccttc gacgagcagc cggtggaaga agcgctcacc agccgcctgc tcaagagcaa
     4621 ctgtcccgtc accggccagc ccgactgggg cagcgtgcag atccgctacg ccggcccgca
     4681 gatcgaccag gccgggctgc tgcgctacct catcagcttt cgccgccaca acgacttcca
     4741 cgagcactgc gtggagcgca tgttcaccga tatctgggag cgctgccggc ccgcccggct
     4801 gagcgtgtac gcccgctaca cgcgccgcgg cgggctggac atcaacccct ggcgcaccag
     4861 ccaccccggg ccgctgccgc ccaacgtgcg cacggcgcgc cagtagggcg gctcgagcgg
     4921 gcagcggccg ccctgcatcc gacgcatgcg gcttggcgtt caaagacctg acccacgaca
     4981 ccggcagcgc cggcgcgagc catgacccat gggtcgtatt gaacctagaa acggcagttg
     5041 gccgagcacg gcacgggcgg gcgcgtccaa ccgccgtttc tgggttgaat gcgagctcgc
     5101 atcagtcgtg gtcggtgaag cgcgtcagcg tggccagtgg cgggaacgcg ccggcgcgct
     5161 gctgcttcat ggccgtcacg gcctcgcgca cgtgggcgtt gctccagatg gcggcctgca
     5221 cccagcccat ctggcgcagg ctgtcgtcga ccgtatggtc gcgcgcgtag tggatcacct
     5281 gcttgctgcc ccagatggcc accggcggct tggcggcgat ctcgcgcgcg cactgcatgg
     5341 cggcggccag gcaggcgtcg gcgctgtcga acacctcgtt gaccagaccg acctcgcgtg
     5401 cgcgcgcggc cggcatgcgg cggccggtgt aggccagctc cttgaccacc gccagcggca
     5461 ccagcttggg caggcgctgc agcgtgccca cgtcggccac catgccgatg ttgatttcct
     5521 gcacgcagaa gaaggcgtcc tgcgtggcat agcggatgca ggcggccgtc accatgtcca
     5581 ccgcgccgcc cacgcagccg ccctggatgg cgacgatgac cgggatgcgc agcgtctcca
     5641 gtcgggtgaa ggtggcctgc aggtcggcca gcaggtcggc gatggcggcg cgcccttcgg
     5701 ggtgggcgtc gtccatctgg atggcgccgc caaagacgtc gagcgccatg ccggcggaaa
     5761 aatgcttgcc ggtgctggag atcaccagcg cgcgcgccgc gccgtcgcgc tgcagcagct
     5821 ccagcgcctg gtcgagctcg cgccagaacg ccggacccat ggtgttcatg gcctcggggc
     5881 gactgagcac caggtgggcg atgtggtcgg aaacggaaag ctgaaaacag gtcagggcag
     5941 gaatgttcat acccgcactg tagtggacgc cagcgccccc agccagtcgc agcggagacg
     6001 gccggcggcg tgggcgcggg cgcgccagcg gacgtacagc gccggcgccc acatgcccag
     6061 cagacacagc ggccaggccc acagcacctc gtcgaacagc agcaccggca accagccgat
     6121 ccagcccatc atccacaggg tcagcacggc gtcgaagctg tggtactcca gcgggtggct
     6181 cggcttgtgc gccaccagcc aggccttgat gcgttgcaat tcgttcagac accacgcggt
     6241 cagcatgatc gtctcctgtc gtgccgtcag gaaactgtaa gcttttgccg ggggcaggtc
     6301 aaaccgtgcc ggtgaacagg acggcgcgca gtctcggaat gctcagtttt caggagctgc
     6361 cggcgcttgc tggaacagcg gcagatcgtg attcgacgcc tcatcgctgg cggccgacag
     6421 ccgatcggcg cgcaccagcg agccgacccg cacgcccagc aggcgcagcc ggcgcgacag
     6481 ctcgacccgc ttgaggcact ggccggcgat ctggcggatg cggcgcgcat cctgcgtcgg
     6541 ctcggccacc gtctggtcgc gcgtggcaat gcgaaagtcg tcgaagcgca gcttgatgcc
     6601 gatggtcttg ccggcatagc ccttgcgctg caggtcggcg gccaccgcct cggccagccg
     6661 ggtgaagacg gcccccagct cggcccggtc gtgcacggcg tgcaggtcgc gctcgaaggt
     6721 ggtttcgcgg ctcaggctga ccggctcgct gtcggtgacc accgggcgct cgtcgcggcc
     6781 ccaggcggcg tcgtgcagcc aggcgccgtg gctggcgccg aactgggcga tcagccaggc
     6841 gcgctcgcgc gccgccagat cgccgatgcg gtggatgccg tgctgctgca ggcgcgcgtc
     6901 ggtcttgggg ccgatgccgt tgatcttgcg gcagggcagg ggccagatca ggctttgcag
     6961 gtcgctgtcg cgcacgatgc tgatgccgcc cggcttgtcg aactcgctgg ccatcttggc
     7021 cagcagcttg ttgggggcca cgccgatcga gcagctcagg ccggtggcgt cggcaatgct
     7081 tttttgcagc agccgcgcca gcacgcgtcc gccttcgcgc tggccgccgg gcacctgggt
     7141 gaagtcgatg tagacctcgt cgatgccgcg atcttccatc accggcgcga tgtccaggat
     7201 gacgcgcttg aaggcgcgcg agcagcggcg gtattcgtcg aagtcggccg gcagcaggat
     7261 ggcctgcggg cacagccgcg ccgccttcat caggcccatg gccgagccga tgccgaactg
     7321 gcgcgccgcg tagctggcgg tggtgatgac gccgcggccc acgtagtcgc gcagcagcgg
     7381 aaagcagtcg accgggatct cggccagatc ggccgccgtc cagacgcgct cggggcgctg
     7441 ccggttgagc cgcgcgatca gctcgtcctc gcgccgccgc ccgccgccga tgaccatcgg
     7501 caggcccttg agctgcgggt agcgcagcca ctcgacggac gcgtagaacg cgtccatgtc
     7561 caggtgggcg atgcggcgcg gcagcggttc ggtcacgcgc ccaagtgtag ggcggcggcg
     7621 ccgcgccggt gccggccggc cgcggctgga acccctttca gtcccgggcc ggcagcacgg
     7681 tgccgacgca ttcgccgaag ccgatgcgcg ctgcgccggg ccttgcgcac cagccgcgca
     7741 gcacgatggt gtcgccgtct tccagccagc tgcgggtttc gccgttgggc agggcgatcg
     7801 ggtgcttgcc gccggtggtc agctcgatca gcgcgcaggc ctggtccagc gtggggccgg
     7861 acagcgtgcc cgtgcccagc aggtcgcccg gctgcaggtt gcagccattg acggtgtggt
     7921 gcgccaccat ctgcgccgcc gtccagtagg cgtggcggta gttggtgtgg gtgatgcggg
     7981 tgggcgggct gcccgccgcg cgcatgcggg cggtctggat cagggcttcg agttcgatgt
     8041 ccaggccgcc ggccgcgctg tgggccgggc tggccaggta gggcagcggc tgcgggtcgt
     8101 ccgccggccg ggtgaacggc gtgcgaaagg gcgccagcgc ctccatcgtc accacccagg
     8161 gcgagatggt ggaggcgaag ttcttggaca ggaagggccc cagcggctgg tattcccagg
     8221 gctggatgtc gcgcgccgac cagtcgttga acagcaccag gccgaacaca tgcgcctcgg
     8281 cctcgtccag ggcgacgggc gcgcccagcg cgttggaggc gccgatgaac acgcccagct
     8341 ccagctcgat gtccatgcgg gcgctgggtt tgaggctcgg cgcgtcggcg tcgggcggtt
     8401 tgacctgccc gcgcggacgg tgaaacgcct ggcccgacac cccgatcgac gaggagcggc
     8461 cgtggtagcc gatcggcacc cacttgtagt tgggcagcag cgggttgtcg gggcggaact
     8521 gcttgccgat gttggtggcg tggtgcaccg aggtgtagaa atcggtgtag tcgccgatgc
     8581 ggcagggcag gtcgtattcg gcctcggcct gcggcaccag gcagggttgc agcgcggcct
     8641 gctggggcga gcccgattgc aatgctttcg atagcgcctt gcgcaagctg gatgcagcct
     8701 ggtggcccat cgacatcaat cgattgaggc tgggttcggc gcccgcctgg gctgcggcct
     8761 gggcctcgcc gtggaacagg ccggcctgcg ccgccgccgc cagatcgacg atctggtcgc
     8821 cgatggccac gccgccgcgc cagggctcgc tgccgccgcg ccggcggaag atggcgaacg
     8881 gcaggttctg gatcggaaag tcggtgcggc cgtcgttggc cgaggtgacc cagctcgtca
     8941 ggtggggatc atgggtgtcg ttcagcgtgg acatgggggc tcctgcaagg cggctggcgc
     9001 gtcgcggcgc caggcggtcg cgcgaggata gcgcagccac gccggcggcg gcgcgcccgc
     9061 gccgcaaggc ccgtcgccgc ggggggtgct gccggcgcgg cccgggccgg cgggcgccgg
     9121 ccagcgcgca acgggattcg atgcgataat gctgggtttc gcggcagggc acaggcccac
     9181 gcgattcatc agagcagacc gcatcttcct tcctgcctgc ggccgccacc ggtttttcgg
     9241 cggccagcat tccagccact ttgggccctc atcggggcca ggtcgttgtt tccgaagacc
     9301 agccgccgcc accgtgggcg ctgggacttc gcgctgtcgg atggtgggtg tcgattcatg
     9361 aacccggcgc gcccaggcgc gccaccatcc gacacgccgg gcgcgcaggg cgccgtggcg
     9421 tgcgagaaag tgcatgtccc atggcctcgt tcgaggaaaa ccacgtcctg acgctggaca
     9481 gtcaagcgcc gcaagctact gaaaacgcag catccgacaa tcccttccag gccctcggcc
     9541 tggcgcccga gctgctggcg gcggtggccg agctgggcta cagcgcgccc accgacgtgc
     9601 agcggcaggc cattccgctg gcgctgcagg cgcaccccga gggcgccaac gacctgatgg
     9661 tcagcagcca gaccggcagc ggcaagacgg cggccttcct gctgccggtg ctgcacaccc
     9721 tgctggacat gcagcgcgag gcccaggcgc gcgagcaggc cgcctgggac gcccgcgtgg
     9781 ccgaagccct ggcgcgcggc gagccggcgc cccgcaagcc gcgccgcaag aacccgctca
     9841 acccgcgcca gttcaagccc gccgtgccgg gtgcgctggt gctgtgtccg acgcgcgagc
     9901 tggcgcagca ggtggcgcgc gacgccatcg acctggtgcg ccactgccgc ggcctgcgca
     9961 tcgccaacgt ggtgggcggc atgccctacg gccagcagat cgcccagctg cagaacgccg
    10021 ccctggtggt ggccacgccg ggtcgcctgc tggacttgca gcgcagcggc cagatcgtgc
    10081 tggatcaggt gcgctttctg gtggtggacg aggccgaccg catgctcgac ctcggctttg
    10141 ccgacgccct ggccgagatc caccagctga ccgcccagcg ccagcagacc atgatgttca
    10201 gcgccacctt cgcggcgccg attcagcagc tggcgctgcg cgtcatgcac gacggcggcg
    10261 cccgcgtgcg caaggtgcag gtggccacgc cgcagcagag ccacgccaac atccgccagg
    10321 agctgtactg ggccgacagc cccgagcacc gtcgccgcct gctggactac tggctgcgcg
    10381 acccgtcgat cgaccaggcc atcgtgttcg cctgcaccca gatcgagtgc gaccagctgg
    10441 ccgccgacct gcagcaaagc ggctttgccg ccgtggcgct gcacggcgcg ctcagccagg
    10501 cgctgcgcaa ccggcgcctg cgcgcgctgc gcgagggcaa ggtgcagatc ctggtggcca
    10561 ccgacgtggc ggcgcgcggc atcgacgtgc ccagcatcac ccacgtcatc aaccatggcc
    10621 tgccgatgaa ggccgaggac tacacgcacc gcatcggccg caccggccgc gccgggcgcg
    10681 acggcctggc ggtcacgctg gccgagttcc gcgaccgccg ccggctggcc gagatcgagg
    10741 cctacacgcg ccagccgttc cgtccgctgg tggtcgccgg gctggagccg acgcggcatt
    10801 tcccgcccgt cagcgagccg cgcgcgccgc gccggccggc cgcgcacgat ggcggggcgc
    10861 gccgcgctgg cgggccgcgc ggggctgccc ggccgacccg cgatcgtcag gccgaattca
    10921 gccctgccgc ccgtctggcg ggcgccggca gctatcaaaa caagagcagc ccgggcgcgt
    10981 cggcgcgccc gcgtgcggcc gccggccggg cccgcgccgg tggcgcgccg cgcccctaca
    11041 ccccggggcg cgcgggtcgc tgagccatgg gcgccgacgc gtcgcgctgg cgcattgcgc
    11101 cgctggccgg cgccgacgtg ccggcgctgc tgcaggtgca ggccgcctgc tacggcccgg
    11161 cgctgctgga agacgccgcc gtgtacgccc ggcgcctggc cagcccggcg cactgcggcc
    11221 tgggcctgca cgccggcggc gcgctggcgg cctacctggc ggcgcactgg tcggtgtggg
    11281 gcgcggtcac gccgctgcac ggcgacttcg cgccgcaggc cgcggccgac acgctctacc
    11341 tgcacgatct ggcggtgcgt cccgagcacg ccggccaggg cctgggccag cgcctgctgg
    11401 cggcggcctg gcgtcaggcg cgcgcgcgtg gcgtgcgtca ggcggcgctg gtgtcggtgc
    11461 agggcacacg cgagttctgg cagcgccagg gcttcgtggt ggcgctccgc cagccgccgg
    11521 cgctggccag ctacggcagc caggcgctgt acatgacgcg caggctggac gacgccgccg
    11581 agcctggatc cacaggcccg atcaacggtc gcgcgccagc acgcgccccg ggttcatcag
    11641 gccctgcggg tccagcgcct gcttgatggc gcgcatcagc gtcagcgcca ccggcgactt
    11701 gtagcgcggc agcagcgccg ccttcaggct gccgatgccg tgctcggcgc tgatcgagcc
    11761 ctggtgccgc gccacggcgt cgaacaccag gaggttgacg cgctcttcct gctggcgcag
    11821 gaactgggcc gcgtcgccgc cttcgggcgc ctgcacgttg aagtgcaggt tgccgtcgcc
    11881 caggtggccg aacacgacca gccgcacgcc cgggatctgg cgcgccagca gggcttcggt
    11941 ctcggccacg aagtcgggca cgcgcgacac cggcacggcg atgtcgtgct tgatgttcag
    12001 gccttcttcg gcctgcgcca gcggaatgct ctcgcggatg tgccacagcg cacgggcctg
    12061 ggcgatgctt tcggccacca cggcgtcgct gacgcagccg gcgtccagcg cggcttccag
    12121 cagcggctcg aagcgcgcac gcgcatgggc ctggctttcc tggtcggcgt tttccagcaa
    12181 cacgcaccag ggcgtgtcgc gccacagcgg cacgcgcagc tgcggcaggt gtcgatccac
    12241 cagaccgagc gcgaattggc ccatcacctc gaagccggtc agccccgcgc ccaggtggcg
    12301 ctgcgccagc cccagcagct ccagcgccgc cgccagcgag ggcacggcgg cccaggcggt
    12361 cagcgtggcg gcggggcgcg ggtagagctt catcgtggcg gcggtgatga tgcccagcgt
    12421 gccttcgctg ccgatgaaca ggtcgcgcag gtcgtagccg gtgttgtcct tgcgcaggcc
    12481 cgacaggccg tcccagacct cgccctgcgc ggtcaccacc tccagcccca ggcacagctc
    12541 gcgcgtgttg ccgtaacgca gcacctgcgt gccgccggcg ttggcgccca ggttgccgcc
    12601 cagcatgcag ctgccttccg agcccaggct gagcgggtac agcaggccgg cgtcttgcgc
    12661 cgcagcctgc agccgctgca gcacgcagcc ggcctcggcc gtcaacgtca ggttggcgcg
    12721 gtcgatggcg cgcaccctgt ccaggcggcc caggttcagc agcagctggg tgccgctgtc
    12781 gtccggcacc gagccgaccg acaggccggt gttgccgctc tgcggcacga tggccacgcc
    12841 gtgcgcggcg caggcgcgca ccaccgctgc cacctcggcc gtgctgcccg ggcgcaccac
    12901 cgccagcgcg cggccgcgca cgcggcggcg ccagtcctgc tcgtacgccg tcaggtcgcc
    12961 ctcggtcagc acatggggtg cgcccaccgt ctggcgcagc agatccagca agggggtgtc
    13021 ggtggtcatg tcaataactc ctgaattgat agctgccagc gcttgccggg caagcgctgg
    13081 atgccgaaat caatgcgaaa ccgcggggcc ggcggcggcc gcgcgcgccg ctcgctggcg
    13141 cagccgcacc tgcgccgcac aggcggcgaa cagcagcagc accagcgcca cctgcgccag
    13201 ggcgcagcgc gccgacacac cgtggtcgct ggtggcgcgc accacgcctt cgacgaagta
    13261 cagccagacg aacagcgcca ggccgcgaca ggtctgcatg cgatagcgca gcagcccggg
    13321 cagcgccagc gccagcggca gcaccttcag ggccagccac gagccgcccg ggcgcagcgg
    13381 cgccagccac agctcccacg ccaggccgag cgccatcagc gccagcaggc tggcgacggc
    13441 gacgacgcgg gtggcctgca cggcaggcga ggggtgggcg ggcatgggcg gtggcatcat
    13501 aacgagcatg cagttcgatg cgctggcggc gcgggcccag gcctttctga ccgagctcac
    13561 cgactttccg gtgcggccca ccgcgcacac gctgtggcag cgcttccggc aggaccggct
    13621 gggcctgacg gccagcgcgc tgaccttcac cacggtgctg gcgctggtgc ccttcgtggc
    13681 cgtggcgctg tcggtgttca ccgcctttcc ggccttcggg cagttgcagc aggcgctgca
    13741 gcagtggttg atcgacagcc tgatcccggc cagcatcgcc gacagcgtga tgggctacct
    13801 gacccagttc gccggccagg ccagcgagct cgggctgctg ggctttgccg ggctgaccct
    13861 ggtgtcgctg tcgctcatgc tgaccatcga caagacgctc aaccagatct ggcgcagcag
    13921 ccagacgcgc ccgctgagcc agcgcatcct ggtgtactgg acggcgctga cgctggggcc
    13981 gctggtgatg ggcgccagcc tggccatcag ctcctacgtg gtgacggcct cgcgcggctg
    14041 gctgcccgac ctgccgggca gcgtggacgc ggcgctggcg ctgctgcagc tgctgctgat
    14101 ggtgggcagc ctgacgctgc tgtaccgcct ggtgccgaac acgccggtgc gctggcgtca
    14161 cgcgctggcc ggcgcgctgt tcgtcagcgt ctgcctgggg ctggcgcggc agggactggg
    14221 gtggtacctg acgcgcatgc ccacctattc ggtcatctac ggtaccctgg ccacgctgcc
    14281 gatcctgctg ctgtggatct acctggggtg gctgatcgtg ctgttcgggg ccgtcatcgc
    14341 cgcctacctg cccagcctgc tgtcgggcgt ggcgcggcgc ggcggcgggc cgggctggga
    14401 cttccggctg gcgctcgagc tgctgcaggc gctggccgcc gcgcgcgatg gcgccgggcg
    14461 cggtctgacg ctgccgcagc tggcgcaggc gctgcgggtg gacgctctgc agctgtctgc
    14521 ggccatggcg gcgctgaccg ggctggactg ggtcggccag ctgggcaccc gcagcgaaaa
    14581 cgaggagccg cgccatgtgc tgctgatcga gccgcgccgc cagctgctgc gcccgctggt
    14641 ggagcgcctg ctgctggcgc ccgacgaggc gctggccggc tggtgggcgc gtggcctgcg
    14701 cagcgaggcg acggtggccg atgtgctgtc gccggcggcc accgattcat gagaaacatg
    14761 gcctgagccg gcgaccagca agcgccagca gctattattt ttgaactgaa ggacgcactt
    14821 cgcccagcct gccgcggggc gcctggggcg ccgcggccgg tgccgccagc aggcggcgca
    14881 ctggcgctgg cagccccagc gccggccatt cgtcggcgcc gaaccagcgc ccgtcggcgt
    14941 ggcgcagatc ggtggcgcgc agccgcacgc tgaccgggtg caggtccagg tcgcggtgcg
    15001 tcagcacatg gcggcgcggc ggctcggtga cggcctgccc gtgcacccgc gccggcaggc
    15061 tcgccagcag ggtcgcccga tcgtcgaaca gcggcaggca gaacaggcgc gcccagatgc
    15121 cgtgctcggg ccgctgctgc aaccacacgc ggccctgcgc gtcgcgcgcc agcagcagcc
    15181 acagctcgag ccgctggcgc gccacgcgcc ggcgcttgac cgggtagcgc tcgggctggc
    15241 ctgcggcggc cgccacgcag aggtcgtgca gcgggcagcg cgggcagtcg gggcggcgcc
    15301 ggtggcacac gctggcaccc aggtccatca ggccctgggt gtagaccggc atggcgcgcc
    15361 gcaggtcggt gcgcggcagc agggcctcgg cctgcaccca cagggcctgc tcgtggcgcg
    15421 ccagcgccag gtcgtggcca aagcccaggg cgcgcgtcag cacgcgcttg acgttgccgt
    15481 ccaggatggc cgcgcgctcg ccgaagcaga acgccgcgat cgccgccgcc gtcgagcggc
    15541 cgatgccggg cagccgcgcc agctcggccg cgctggccgg aaagcgcccg ccatggtgcg
    15601 ccaccacgtc gcgcgcgcag cggtgcaggt ggcgcgcacg gcggtagtag cccaggccgc
    15661 tccacaggcc cagcacctcg tcctcgctgg ccgcggccag ccgttcgacg tcgggaaagc
    15721 gcgccagaaa gcgcgggtag taggccagca ccgtggtcac ctgggtctgc tgcagcatga
    15781 tttccgacag ccacacgcga tagggatcgc gcgtgccttg ccagggcagg tcgtggcggc
    15841 cttggcggcg ctgccaggcc atgacgcgcg cggccagctc ggcgccgccg ccgttcacgc
    15901 cgggacgacc tcggtcagct ccggcggggc ggtgtcggca gcgtcggtgg cgctggcgcg
    15961 gcgcagctcg tgccccagct cgcgcaggcg ctgggccgtg gcctgcaggg cctgctgctc
    16021 ctgctggagg tcgtggatgc gctggtccag gctgccggcc gcgtcctgga tgcgttcgac
    16081 cgcgtcgatg cggcgaccgt aggcgcgtcg ccgctcgcgc agctgggtgt cgagctggct
    16141 ggtggtggcc ttgttccaca gctcgatgtc gcccagggcg gtttcgaaga tcgagcgcac
    16201 ccgcgtcagc agcgcacgca ccagcttctc ggagaagccg gcgcgcgcca gctggagcat
    16261 gtgacccacg cccaggtagc gcaggtggct gcgctcgacc gcgtccaggt cgctgacaaa
    16321 gcgctccatg gcgggcggct cggcggcctg cagcgcaaac ccgtagtcgg tgttgagctg
    16381 gacaaacgcc gccttcagca tggcgtggat ctcgccttcc agcgcctgca cctgccggat
    16441 cacgccgcgc agccgctcaa agccgtcggc gtaggacttc ttgatgccga gcttcaggcc
    16501 cggctcgcgc agggcggcgt tcagctcggc cagctcgcgc ttgatcgacg cattgcccag
    16561 cagctggaac acctcgcgca gcagcttcag gtgcaccgaa cgcacggcca gcacgctggc
    16621 ggcgctgcgg tcgaagtcgg ccttctcctg ctcgatgcgc aggcgcatct ggtggatcac
    16681 ctggtggttc ttgccgcgca ggccgttgag ctcgatggtc tggtcggaca gctcgcgccc
    16741 gcgcatggcc agggtgcgtt ccgcctcggc ctgggcacgg gccacgccgg caccgatggc
    16801 gtcgtgcagc atggcgtggc gcgcgcccag cacctgctgg cccagcagat cctcgaagtg
    16861 cggcaggcgg ctgtcggcca gcagggccgc atcgccgcgc accttggcca gcaggccctt
    16921 ctgcgccgac agcgccagca cctgatcgtc ctgcagcgcc agggtgcgcg cggtgtcggc
    16981 gcattggcgg gcgatctgcg cttcgatctg cgccggcgtg ctgagctcgt cccacagggc
    17041 gtcgatcttg ttcagcacca cgaagcgcga actgctgctg tcctgcatgg tggccacatg
    17101 ctcgcgccag atgccgaggt cggacttggt cactccggtg tcggccgcca ggatgaacac
    17161 cacggcctgc gcctgcggga tcaggttcac cgtcagctcg ggttcggcgc cgatggcgtt
    17221 cagccccggg gtgtccagca ccaccaggcc ctgcctgagc agcgggtgcg ccatgttgat
    17281 gagggcatgg cgccagcgcg gcacctccac caggccctgg gcgttgaccg gcgggttgtc
    17341 ttccgggcgg tcgtcgtgcc agaagccgag cgcacgcgcc tgcgcctggt cgacgtaacg
    17401 cacctcggat accttctcca tggcactggc cagctgctgc gggtcgttca cgtccagatc
    17461 gacccgcgtc cactgctccg gcgccatgcg ccattccatc agggcctggg gctgcaggcg
    17521 cgtttcgatc ggcagcaggc gcaggcaggg cggcagctcg gcgtcgtagg ccagctcggt
    17581 cgggcacatg gtggtgcgcc cggcgctggc cggcatgatg cggcgaccgt agccggcaaa
    17641 gaacaccgca ttgatcagct ccgacttgcc acgcgagaac tcggccacga aggccaccat
    17701 caccttgtcg gagcgcaact gctcgaccag gcgctgcagg ctttcgtcgg tcgcgtcgtc
    17761 cagcaggcgc tgctgccgca gccaggccgc caggttcttc aggcgcgaac tcatttcgcg
    17821 ccgccatgcc ccgtgcctgt cgaactgttc gctgaaagcc gccaccatgc cactccggat
    17881 cgttgggacc atattacatg gtgtaacccg gcttggcatg gtcacgacgc gcgaccgggt
    17941 ccgcgggtcg tgagcaagtt cgcgtcaggc gcgctggcag cgggggcaga agtaggtgga
    18001 acgctggccc tggcggatca gccgcaccgg tgtggcgcat tgcagacagg gttgcccggc
    18061 gcgcccatag accgcggctt cgagctgaaa gtgcccgctc tcgccggtgg cgctggaaaa
    18121 gtcgcgcaag gtgctgccgc cgcgctcgac cgcccgcgcc agcacctgcc gcaccgccgc
    18181 atgcaggcgg tcggcgcggg cgcgcgacag ccggtcggcg cgcacggtgg gccggatgcc
    18241 ggcctgaaac agcgcctcgg aggcgtagat gttgcccacg ccgaccacca cgtcgccggc
    18301 cagcagcacc tgcttgatcg gcgcacggcg gccgcgcagt ccggcatgga aggccgccgg
    18361 atcgaaccgc tcgcccagcg gctccatgcc cagcgacgcc agcaacttgc gcgccgggtc
    18421 gagctgcagg ctggccgacc acaccacggc gccgaagcgg cgtgggtcgt gcaggcgcag
    18481 cgtgccgtgc gtggtcacca ggtcgaaatg atcgtgtcgc cccggcgccg ccggtgcggc
    18541 gtcaaagctc aggctgcccg acatgcccag gtgcagcagc agcacgccct gatcgaggtg
    18601 cagcagcagg tacttgccgc gccgccgcac gtcctgcacc gtgcggtcct ccagctgctc
    18661 cggggcacag cccagcggcc agcgcagcgg cagaccgggg cgcgcggcca ggatgcgcgc
    18721 gtcgcggatg cggtcggcga agctcagccg cgtgacttcg acttcaggca gctccggcat
    18781 ggcgttcggg gcgggcagtg gcatcgctgg gcatcattta ttatggatcg atgatgcggt
    18841 ttcaacggct acttggcggg gcggtgctgg cgtgtgcggc atgggcggcc gccgcccagg
    18901 ctcctgctgc accacaggcc ggcgctgccc aggcgggcgt gcccgagacc tcgcgtctga
    18961 acgccaggct gctgtacgag ctgctgctgg ccgagctgct ggcgcgcgag ggcgaggccc
    19021 agcagggcgc cgcctacatg ctgaacgcgg cccggcgcac gggcgacgag gccttgttca
    19081 agcgcgccac cgagcttgcc atccagtcgc gcgccggccc tgcggcactg gaagccacgc
    19141 gggcctggcg acaggcgttc ccggattcgg cccaggccgg ccagtacgag ctgcaggtgc
    19201 tgatcgtgct cggtcgggtg gccgagaccg aggagccgac gcggcatttc ctggccaccc
    19261 tgccggaggc cgagcgcctg agcttcatca cggcgctgcc cgcgctctac cagcgcgtgc
    19321 ccgacaaggc cgaggccgca cgcgtgatcg agcgcgcgct ggacggcgcg ctcagccagc
    19381 cggcgctggc gccggcggcc tggaccagca tcggccggct gcgtctgcag gccggcgaca
    19441 aggccggtgc gttggcggcc gcgaccctgg gccagaaggc cgacgcatcc tcggagtggc
    19501 cggccctgct cgggctgcaa ctgatgggca gcggcgaaac ccaggccgaa gccctggtcg
    19561 agcgccatct gtccactgcg caggcgcggc ccgagctgtt catcgcctac gcgcgtgcgc
    19621 tgatcgagca gggccgcgga ggcgacgccc atcgccagct ggaccgcctg atcgcgcggg
    19681 cgccggagca cccggaggcc tggctggtgc gcggggcgct gtatgccgac gagcgcaagg
    19741 acgccgaggc cgaagccgac ctgcgccgct acctgagcct gctggaggcc acaccggcga
    19801 cgccggacgc cgagccgccc gaaggcagcg cccacgcccg catgatgctg gcgggcatcg
    19861 ccgagcggcg cggcgagctg acgcaggcgc agcagtggct ggcctcggtc gattcgcccg
    19921 agcaggcact ggccgtgcag acgcgcctgg cgcagatcct ggcgcgccag ggtcggctgg
    19981 aggaggcgcg ccagtccatc cgtgcggcgc ccgagcgcag ccccgaggat gcccggctca
    20041 agctgctggc cgaagcccag ttgctgcgcg atcacggcca gcccgggcag gcctacgaac
    20101 tgctcaacgg cgcgctgcaa cagacccccg acgaggaaac cctgctctac gaagccgcca
    20161 tggcggccga gcgcgtggga aggttcgagg tgatggaacg cctgctgcgc cgcctgatcg
    20221 aactcaagcc cggcgcctcg cacgcctaca acgcgctcgg ctatgcgctg gccgatcgca
    20281 acctgcgcct gcccgaggcc agaaccctga tcgagaaggc ggtgcagctg tcgcccaacg
    20341 acgcctacat ccaggacagc ctgggctggg tgcagtttcg acagggcaac ctgcgccagg
    20401 cacgccagac cctcgaagcc gccttccgca agcgccccga cgccgagatc gccgcccacc
    20461 tgggcgaggt gctgtggatc ctgggcgagc gcgaggccgc gctgcgcgtg tggcgcgaag
    20521 gccagcgcct ggaggccgac aacgagacgc tgcgcaaaac cctgctgcgt ctgcgggtgc
    20581 ggctgtgaag gccgccgtcc ggcgcctcgg cggcgccctg tgtgctattt taatgatagc
    20641 tggttgcgca caccagacgg gcgcaaacgc cccatttgac tcgcaaacca gccattggag
    20701 cgggcgcctg gggctgaccg tggcgtccga gccgccgcag tcgtactcgg ccggttttgc
    20761 gctcagcggc aacgccctgg tgggcgagct gatgctcacc tcgcccctgg gcagcgccct
    20821 ggccgtgatg cgctggcagc ccggtcaggc gctgctgcag cagggcgagc agaccgcccg
    20881 cttcgaatcg ctcgacgcgc tggtggagca ggtcaccggc acgccgctgc cggtgcgcgc
    20941 gctgtttggc tggctgcacg gtcagccgca gtcggtgccc ggctggcagg ccgacctgag
    21001 cggtctgccc gacgggcggc tgttcgccca gcgcctgatg cccctgccca cggccgaatt
    21061 gcggctggtg ctggaccgct gaggcgcgga accgggatgc gcgcgttcta cgacctgccg
    21121 gcgccggcca agctgaacct gttcctgcac gtcaccggcc ggcgcgcgga cggctatcac
    21181 ctgctgcaat cggccttcat gctgatcgac tggtgcgaca ccctgcacgt cgagcggcgc
    21241 gcgggcggcg ccctcagccg ccaggacctg gggccgaccc tgccggccga cgatctgatc
    21301 ctgcgcgccg cgcgcgcctt gcagcaggcc agcggctgcg ccgagggcgc gcacatcatg
    21361 gtgcacaagc gcattccggc acaggccgga ctgggcggcg gctcgtccga cgccgccacc
    21421 acgctgctgg cgctcaaccg cctgtggcgc cttggcctga accgggcgca gctggcgcgc
    21481 atcggtctgg cgctgggcgc cgacgtgccg ttcttcctgc atggcacaca tggctgggtg
    21541 gagggcatcg gcgagcgcat ccgcacgctg caactgccgc cggcgcgttt tgttgtggtg
    21601 aagccgccgc aggggctgga aacggcagcg atttttcgcg acccggccct gaagcgagac
    21661 agcaaacctg ctataatcgc aggctttgct gcagacccgt atggctttgg ccacaacgac
    21721 ctgcagccgg ttgccgagcg gctttgtccg caggttggcc aggggctggc gctgcttgcg
    21781 cagcatggcc tgcatggcag aatgaccggc tcgggcagtg cggtgttcgc tccggtggtg
    21841 ccaggcgcca tgaccggcgt gaccgaggtg caggttccgc ccgactgggg cttgcgctgg
    21901 tgcagcaatc tggagttcca cccgcttggc ggttgggcgc caggtgaagt ttgaaggggg
    21961 ttggcccggt ggtatggggt cggtttcctg tgtaggggag tagccaagtc ggttaaggca
    22021 ccggattttg attccggcat gcgagggttc gaatccttcc tcccctgcca aatgactgtg
    22081 accgggtggc gtgccagcag gcacgccatt tcggcttctt gccgtaccgt ttcagtgtca
    22141 ttccgccgca aagcgcatgc agcccaccga ctcctccgac ttcctggtgt tcaccggcaa
    22201 cgccaacccc acgctggccg ccgagatcgc ctacaacctg ggcatcagcc tgggcgcggc
    22261 cgacgtaggg cgcttttccg acggtgaggt gaccgtcgag atcaagcaga acgtgcgcgc
    22321 gcgcgacgtg ttcgtggtgc agtccacctg cgcgccgacc aacgacaacc tgatggagct
    22381 gctgatcatg gtcgacgcgc tcaagcgcgc ctcggccgaa cgcatcagcg ccgtgattcc
    22441 ctatttcggc tatgcgcgcc aggaccgccg gccgcgttcc acccgcgtgc cgatctcggc
    22501 caaggtggtg gccaacatgc tgcaggccgt cggcgtcaac agcgtgctga ccatggacct
    22561 gcacgccgac cagatccagg gcttcttcga catcccggtt gacaacatct acgcctcgcc
    22621 ggtgctgctg ggcgacctgc gccagcagaa ctacgacgac ctgctggtgg tcagccccga
    22681 cgtcggcggc gtggtgcgcg cgcgcgcgct ggccaagcag ctcgactgcg acctggccat
    22741 catcgacaag cgccgcccca aggccaacga gagcgaggtg atgcacgtca tcggcgacat
    22801 cgagggccgc aactgcgtcg tcatggacga catgatcgac accgccggca cgctggtgaa
    22861 ggcggccgag gtgctcaagc agcgcggcgc caagaaggtc tacgcctact gcacgcaccc
    22921 gatcttctcc ggccccgcca tcgagcgcat cgccaagggc agcgcgctcg acgaagtggt
    22981 ggtgaccaac accattccac tcagtgccgc ggcggcggca tgccccaaga ttcgccagct
    23041 ttccgtggcg ccgctgatcg cccagacgat ccagcgcatc gcccgcggag agtcggtgat
    23101 gcgtctgttc caggagcagg aaaacctgtt ctgaggaacc attcgaggtt cgcgcggcca
    23161 ccgtggtggc ggtgaaccgt tttcaacccg ggtcgcactg gtcgcggtcg gcccacaagg
    23221 agtacgtcat gaaattcgtc gctttcgagc gcagcaagca gggaacgggt gcgagccgcc
    23281 gtctgcgcaa tgccggcaag gtgccgggca ttgtctacgg cggtgagggt gagccccagc
    23341 tgatcgagct ggaccacaac gcgctgtggc acgccatcaa gaaggaagcc ttccactcgt
    23401 cggtgctgga catggagctg ggcggcaaga ccgccaaggt gctgctgcgc gatgtgcagg
    23461 tccatcccta caagcagctg gtgctgcacg tcgacttcca gcgcgtgtcg gccaagcaga
    23521 agatccacat gaaggtgccg ctgcactacc gtggcgagga agagtccaac gccgtcaagc
    23581 tggacaagtg cctgatcaac cacatcctga acgagctcga aatcagctgc ctgccggccg
    23641 acctgccgga agccatcgag gtcgatctgt ccaagctgga aaagggtgcg accgtcacgc
    23701 tcaaggacat cgcgctgccc aagggcgttg agccggtgct gcgtggccac agcgccgaag
    23761 atacggtgct ggtgtcggtg gtgaccccgg cggccgaggc ggctgaaggc gaagccccgg
    23821 cggccgccga aggcgacaag aagtgatcgt tgcgcgcggg ctcagcctgc cgtgatgacc
    23881 accgacggcc cgcggacagc gggccgtttt catgggcggt cctgcgggcc cggctgtggt
    23941 cacgccgata atcgcaaccc atgatcaagc tgttcgtcgg cctgggcaat cccggcccgg
    24001 aatacgctgc cacgcgccac aacgccggct tctggtggat cgaccaggtg gcgcggcagc
    24061 tgggcgtgcg gctggtgccc gagcgcgcct ttcacggtct ggtggcgcgc gccagcgtgc
    24121 agggcgccag cgtgtggctg ctgcagccgc agaccttcat gaacctgtcg ggcaagtcgg
    24181 tggcggcgct ggcgcgcttc tacaaggtgg cgccgcagga aatcctggtg gcgcacgacg
    24241 agctggatct gccggccggc gaggccaaac tcaagcaggg cggcggccac gccggtcaca
    24301 acggtctgcg cgacatccat gcgcagctgg gcagcagcga ctattggcgc ctgcggctgg
    24361 gcattggcca tccgggaaac cggcaggagg tggtcggctg ggtgctgaag aagccgccgc
    24421 tggacgaaca gatcggcatc gaacaggcca tcgatcgcgc cgcgcgcgcc tttgcgctgc
    24481 tcgcggccgg cgacatgacg gcggccacgc aggccatcca cacctccagg ccaccacgcc
    24541 ccaagccacc gcgcaagccg ccgccttccc cggccgacgc cggtgccagc gagcggcccg
    24601 atgtctagca gactgtcgga cctggaaatc gccggctgca gatcgaccgc agcgcccctt
    24661 tcttggcttc gccgcgtttc gagagcgccg cctttttccc caatggctcg gctgttgggc
    24721 tgcccatccg gcaacaacgg gcctcgctgg ggccgatgcg cgctgtcggc gctccaagtc
    24781 cgacagcctc ctggtcgcag gtgcttcgcg cgcgtcgcat gcctgcccgg ggtggtctgc
    24841 ggcgccgctc aggggcgcca tggcttcggc actgtctgcg cctggcgtcg tgcatcccat
    24901 gcccatccgt ggcgcgccgc aacgtggggt cgggttcctc ctcgttctgg cgcagcctgg
    24961 ggggcggccg ggtgtcccca tggtgtgccg ccgtatccag gcgtcaccga ccgaactttc
    25021 gccgcgctgc gtcgctcaga gtcaccatga aaccttgccc catgcatgtt cctaggctgt
    25081 ccgcggtcgc cttggtgtgg ttcgccctcg gggcgccggt ggcccgcgcc gacgggttgc
    25141 agaacctgga cgccttcatt cgccacagcc gcagcggcag cgcggatttc acgcagaccg
    25201 tgacggcacc ggccaagccg ggtcagccgg cgcgggtcaa gacgtccagc ggtcgcttcg
    25261 aattccagcg tccgagccgg ttccggttcg actaccagaa gccgttcgtg cagaccatcg
    25321 tcgccgacgg caagacgctg tggctgtatg acgccgacct caaccaggtg acgcagcgcg
    25381 cgcaagcgca ggtgctgggg cagacgccgg cggcgctgat tgccgccgcg cccgatctgc
    25441 gtgcgctgca ggccgagttc acgctggcgg cggcgccgga ccgcgacggt ctgcaatggg
    25501 tcagcgccac gccgaagcag cgcgacggtc agctgcagca ggtgctggtc ggctttgacg
    25561 gcgagcggct ggccgccctg gagatcgtcg acagccttgg ccagcgctcg gtgctgaaat
    25621 tcgggcggat cgaggtcaac ccggccttgc cggccagcac cttcgagttc aagcccccgg
    25681 ccggcgccga cgtgctgcgc cagtgagccc gccgcagggt cacccgcgtc acaggcggta
    25741 gaagctctgg atggcgtccc aggcctgggc gggtgtgtcc accacgcggt acagctgcag
    25801 gtcctcgggc gcgatggcgc cttcttccac cagcatgtcc aggttgagca ggcggctcca
    25861 gtagctgctg ccgcacagca cgatcggcac cggcctggct ttgcgcgtct gcaccagggt
    25921 cagcacctcg aacagctcgt ccagggtgcc gaaaccgccc ggaaagacca ccagcgcctt
    25981 ggcgcgcatc atgaagtgca tcttgcgcag cgcgaagtag tggaacttga acgccaggta
    26041 gggcgtgacg taggcgttgg tgtgctgttc gtgcggcagc gcaatgttca gaccgaccga
    26101 gggcatgccg acgtcgtggg cgccgcggtt ggccgcttcc atgatgcccg ggccgccgcc
    26161 ggtgcagatg aacaaccggt cctcggcgcg ctggcgctcg ctgtgggtgg ccaccagccg
    26221 tgcgaactca cgcgcctggg cgtagtagac ggcgttgcgc accgccagct cggccgcggc
    26281 aagggcggcg gcgtcgccgc tggtgcgggc gctggcgagc tgctgcgccg cttcgtcggg
    26341 cgcgacgaag cgtgcgctgc catagaccac cacggtgctg tgcacgccgg cggcgctcag
    26401 gtcgaggtcg ggcttgagca gctcgagctg aaagcgcaca ccccgggttt cggggcgcag
    26461 caggaattcg gggtcggcaa aggccaggcg gtaggccgcc ggttcgagcg gggtgccgtt
    26521 ctgcgcatgt gcctgcaacg tggcccaggc gtcctggatc gaggtgagcg acgacgtctt
    26581 gatgtcttgc gtggtttcca tgggcgcgat cttattgaac ctgtcagcag ccgttgaccg
    26641 cctgccatcg tatggcttcg gtatcgtgaa gtttgaacct ggtccactga acctggtgct
    26701 gaaaacactg aaatcggatc ggcgctgtcc gcccgatacg ggcacgctgt ggcgttgcca
    26761 atgctctcca cagcacgggc tgttgctgcg ccttgcgcct tgcatcgcct cccgtcgaca
    26821 acaaaaaagc cccgggcggg gcttttttgg gggtgcacgc tggcctgcgt cagacgcggc
    26881 ggcggtattc gccggtgcgg gtgtcgatct cgatcctgtc gccctggttg acgaacagcg
    26941 gcaccgcgac ctcgaagccg gtggcgatct tggccggctt gagcaccttg cccgaggtgt
    27001 cgcccttgac ggccggctcg gtccaggtga tttcgcgctc gacgctggtg ggcagctcga
    27061 ccgagatggc cttgccgtcg tagaacacca cttcgaccgc catgccgtct tccaggtagc
    27121 tcagggcgtc gcccatgttg ccggcctcga cctcgtactg gttgtactcg ccgtccatga
    27181 acacgtacat ggggtcggca aagtaggaat aggtgcattc cttgtggtcc aggatgacct
    27241 ggtccatctt gtcgtcggcc ttgaacacca cttcggtgcc catgttggac agcagggcct
    27301 tgagcttcat gcgcacggtg gcggcgccgc ggccgccgcg ggcgtattcg gtccgcagga
    27361 cgatcatcgg gtccttgccg tgcatgatga cgttgccggc gcggatttcc tgggcgattt
    27421 tcatggctgt gattccagtg tgtgaggtga gcgtgggccg ctcgtgcact ggctgtgaga
    27481 aagggcgtgc gcgcggcggc agcgtggcaa aagacagccg cgctcacgac ttggcagctg
    27541 attttgcaaa gccttgaatt ataacgtttg ccgcgccacg aaccgcagca gctgggtggc
    27601 cagatcggtc tgcgcgtgca ggcgctggcg ggccgcctgt acgccgcggc gccaggcggg
    27661 caggtcgggg gcgggcaggg tgccaccgtc gatgccgttc caggcgaggt ggaactggcg
    27721 caggctggcc ggcgcttcca gccagtccag aaaggcgcgc agcttggcgt ggtgggcgtt
    27781 gtcctcgggt tgcgggtaga tgtgccagac caggggctgg ccggcccaca gcgcacgcac
    27841 cagcgagtct tcgccgcgca cgaagttcag gtcgcaggcc cacagcagtt cgtcgtaggc
    27901 gatttgcgtg aaccgcggca ggtatgaaat cgatagctgc tggcgctgac tggaagggcg
    27961 ctgcagccgg ttttcctgct caagcacttg ctccaccgcc tgcctggcgc ggccggcggt
    28021 gaccagcagg cgcgtcggtt cgggcgagcc gcgcagctgc gcgatccagg tcgccagcgc
    28081 ggggggctcg tagcagaaca gcgagatcag ccgctcgcct tgccagtcga tgccttgccc
    28141 ggccagccag gcgcggcgat cgaaggcggc ctggcgcgcc ggcaggtcgt cttcgcgcag
    28201 cagcccaccg gtggccgggg tgaagccggg gtagaagaac cacttggtcc agccgcgggc
    28261 gggcccctgc agcaccggcg agggcaggcc gtgcatgcgc tcgacgtacc cctcggcgct
    28321 caggtactcg aggttgatcc ataccggtgg tttcaagcct ttgtggttcg cagcgcttat
    28381 cctttcagcg ccggcagcta tgaattcagt agcaatctgg cagccgaagg cttccaccca
    28441 gacatcggcc ggtggcagcc cggccagccg catggggtcc agcggtcgcg tccacggcag
    28501 cacctgcacg ccggtcgggc gtgtggccgc gggcgccatc cagtccagcg cgcgcgcgtc
    28561 gtccacccac aggcgcacct gctgctcacg cgccgccagc gccgtcgcca ggcgccagca
    28621 gacgcccagg tcgccgtggt tgtcgatgac ctgacagaag atgtcccagc gcagcggggg
    28681 gtggggttcg ggcacgctgg cattgtgggg caaggcccgc cgtggcggcg ccatggcgtg
    28741 gcttcgtaca ctgcgcacca tgaccgatgc cccggcgccc gacgacgcgc cgcccccgcc
    28801 acccgccgcg ccgcgccacc gccccgagct gctggcgcag gtggcggcgc tgcccggtct
    28861 gcccggcgtg taccgctact acgatgcggc cgacaacgtg ctgtacgtgg gcaaggcgcg
    28921 caacctcaag cgccgcgtca gcagctactt tcagaaaaac cacggcggca cgcgcatcgg
    28981 ccacatggtc ggcaagatcg cgcggctgga aaccaccgtg gtgcgctccg aggccgaggc
    29041 gctgattctg gaaaacaacc tgatcaagca gctggcgccc agatacaaca tcctgtttcg
    29101 ggacgacaag agctacccct acctgaagat caccggcagc gaaggcgtgc cggaccgcgg
    29161 cgagggcgcg ccccgacccg aggcctacgc gcgcatgacc tactaccgcg gcgccaccga
    29221 ccgccgcagc cgctacttcg gtccctatcc cagtgcctgg gcggtcaagg aaaccatcga
    29281 gcttttgcaa aaggtgtttc gcctgcgcac ctgcgaggac acggtgttcg cccatcgcac
    29341 gcggccctgc ctgctctacc agatcaagcg ctgcaccgcg ccctgcgtgc ggctgatcgg
    29401 ccaggccgac tacgccgccg acgtcgccca ggccgagcgc tttctgcgcg gccagacgca
    29461 gcagctgctg accgagatgg aacggcgcat gatggcgcac gccgacgcgc tggagttcga
    29521 gcaggcggcg gcgatccgca accagatcca gtcgctgtcg cgcgtgctgc atcagcaggc
    29581 ggtggaggtg ggtggtgaag ccgacgtcga catcatcgcc gtcaaggtgc agggcggacg
    29641 cgcctgcgtc aacctggcca tggtgcgcgg tgggcgccac ctgggcgatc gcgcccactt
    29701 tcccagtcag gtcgaagatg cgcacgcact ggccggcgag cgcgaggacg ccgagctcac
    29761 cagcgtcgag gcgcaggtgc tggacgcctt catcgcccag cactacctgg cgctgccgac
    29821 gccgccccag ctgatcacca gcgaaccggt gacgcgcacg ctgctgcagg ccttgtcgga
    29881 caaggaagag cgcaggatca gcgccaccca cgcgccgcgc ggccagcgcc gcgtgtggct
    29941 ggagatggcg cagcaaaacg ctgccctgca gctggcgcgc ctgctggccg aagaaggctc
    30001 gcagcaggcg cgcacgcggg cgctggccga ggcgctggag ctgcccaccg aggcactcga
    30061 tcagctgcgc atcgaatgct tcgacatcag ccacaccgcg ggcgaggcca cgcaggccag
    30121 ttgcgtggtg ttcgaaggcc atcgcatggc cagcagcgag taccggcgct acaagatcga
    30181 cggcatcacc cccggcgacg actacgccgc catgcgccag gtgctgcagc ggcgctacgg
    30241 caagatcgcc gaggcgctgc gcgagcaggg caacgccgcc cgcgtgcgca tgcccgacct
    30301 ggtgctggtc gatggcggca agggccaggt cagcgtggcg cgcgaagtgt tcgaggcgct
    30361 ggcgctcgat ctgtcgctga tcgtcggcgt cgaaaaaggc gaaggccgca aggtcgggct
    30421 ggaagagctg gtgtttgccg acggccggcc caaggtctat ctgggccacg actcggccgc
    30481 gctgatgctg gtggcgcaga tccgcgacga ggcgcaccgc ttcgccatca ccggcatgcg
    30541 ggcgcagcgc gccaaggtgc gcgtgggcgg cagccagctg gaagacatcc ccggcgtcgg
    30601 gccccgcaag cgtgcgcgcc tgctgcagcg ctttggtggc gtgcgcgggg tggaggccgc
    30661 cagcatcgac gatctggcca cggtcgaagg catctcgcgt gcgctggccg aggacatcta
    30721 ccgtgcgctg cattgaccgg attgcgcatc aaatcggcat ctggcgcagg ggtagtaagc
    30781 gcaggcagct atcgtttaca tagcatgcag ggccttggcg gagcacgctg cgtgcccgca
    30841 agccgcgtga cacaatcgcc gccatgttct tcaccctgcc cacgctgctg acctggacgc
    30901 gcatcgtcgc gattccgctc atcgtgggtg tgttctacct gccgctggcg ccgcacaccg
    30961 ccaacctggt ggccaccgtg atgttcatgg tgttcgcctg gaccgactgg ctggacggct
    31021 ttctggcccg caagctgaac cagacctcgg ccttcggcgc ctttctcgac ccggtggccg
    31081 acaaattcct ggtctgcgcc agcctgctgg tgctggtgca cctgcagcgc accgacgtgt
    31141 tcgtggcgct gatcatcatc ggacgcgaga ttgccatttc tgccctgcgc gaatggatgg
    31201 ccaagatcgg cgccagcaag agcgtggccg tgcacatggt gggcaagctc aagaccgcgg
    31261 tgcagatggt ggccatcccg ttcctgctgt acgacggcgt gctgctggag cgcatcgatg
    31321 cgcgctggtg gggcaacctg ctgatctggg tggcggcggt gctcaccgtg tggtcgatgg
    31381 tgtactacct gcgcaaggcc ctgcccgaaa tccgcgcccg cgtgcagtga cgcccgcatg
    31441 aacccttctt ccccggacgc cggcggcgcc acgttgatcc gcctgatgcc ggccgtcttc
    31501 gtcgtcatct gggccaccgg cttcatcgtc gcgcgctacg gcatgccgca tgcgccgccg
    31561 ttcagttttc tgctgttgcg ctacgcgttt tcggtggcct gcttcctgcc gtggatcgtc
    31621 ctggcccgtg tggcctggcc gcgcaccggc ctcgaatggc tgcacctggg cgtcaccggc
    31681 gtgctgatgc acggcggcta cctgggcggc gtgtgggcgg cggtcaaggg cggcatgggt
    31741 tcgggcctgt cggcgctgat cgtcggcatc cagccggtgc tcaccgccgt ctggctggcg
    31801 ggcatgggtg gggccggcgc ccgcgtcagc gcgcgccagt ggagcgggct gctgctgggc
    31861 tttgccgggc tggtgctggt ggtgtggcgc aagctgacgc atggcgcgcc gggcgaccac
    31921 gtcaccgcca ccaacctggc ctgggcggtg ctggccctgg tcagcatcac cgccggcacg
    31981 ctctatcaga agcgcttcgt gcgtccctgc gacgtgcgca ccgccaacac cgtacagctg
    32041 ctggccgcag ccctggtgac ggcgccgctg gcctggctgg agcctgaatc cgtgcggtgg
    32101 aacggccagg tggtgggggc gctggcctgg tcggtgctgg gcctgacact gggcggcagc
    32161 tcgctgctgt acctgctgat tcagcgcggc gcggcgtcca gcgtggccag cctgatgtat
    32221 ctggtgccgc ctaccacggc ggtgatggcc tgggcgctgt tcggtgagcc gatcggcgtc
    32281 accacgctgg ccggcatcgc actgacggcc tttggcgtca gtctggtggt gcggcccgcc
    32341 gccggcaggc cctaagctgg tcggcccagg ctcggccgct gtgccctggg cggcacaggc
    32401 accagtcaaa aatcacgttc agccgttgta gcggcgcagc ggcagaccgg atttcccccc
    32461 ggtggcctgg gcaattgagt ctagaatgcg tccctcgccg tctggggatt gcgtcagcgc
    32521 agaaccagtg tcacgtgggt gaccgtgtga ttgaaacgcc ctgctgcggc gatgggccaa
    32581 gcaaaggaaa accgggttgg accggcttgc cagacacgca tgccgttctg gcctggtcct
    32641 tgaatttttt cgagagggac acattcgtga acaagaccga gctgatcgag cacatcgcca
    32701 agaatgcgga catttccaag gccgccgcca cgcgtgccct ggaggcggcc atcggcgccg
    32761 tcaagaccac gctgcgcaag ggtggaacgg tgtcgctggt gggttttggc acctttgccg
    32821 tgggcaagcg tgcggcgcgc tcgggccgca acccccgcac gggcgcaacg attaaaatca
    32881 aggcagccaa ggtgcccaag ttccgtcccg gcaaggcctt gaaggacgcg ctgaactgat
    32941 gccatgtcgg tccggctggg gtgcttagct cagttggtag agcggcgccc ttacaaggcg
    33001 taggtcggcg gttcgacccc gtcagcaccc accagcatcc tccaccgatt gcagcaaagg
    33061 cgaaccccgg ttcgcctttt tcgtttttac gccaccgcgt acaccgagag tctcgcgcat
    33121 gttcgatttc gttcgccggc acaccaagct catcatgggc gtgctgttcc tgctgatcat
    33181 tccgtccttc gtgctgttcg gcatcgaggg ctacacccgt ttcaacgatg gtgccacgaa
    33241 ggtggccacg gtcaacggtc agccggtcac ccagatggaa tgggacagcg cgcatcggct
    33301 ggagatcgac cgcctgcggc agggcaaccc gaacctggac ctgagcctgc tcgattcgcc
    33361 agcgctcaag tacgcgacgc tggagcggct ggtgcacgaa cgtgtgctgg ccgccgcggc
    33421 cgccgacgag catctgatcg ccaccgacgc ccgcctggcg gccgaactgc agcgcgaccc
    33481 caccatcgcc agcctgcgcc gcgccgatgg caccctggac atggagcgct accagcagct
    33541 gctggccgcc caggggctga cgccggccgg cttcgaagcc aatgcgcgac acgagctgtc
    33601 gtcgcgccag gtgctgggcg gggtgtccga gaccgggttt tccagcgcgg cccaggccca
    33661 ggtcgccatg ggggcgtttc aggagcgccg cgaagcccgc gtgcagcgct ttgccccggc
    33721 cgagtttgcc gacaaggtcc agccgaccga cgccgatctg cagagctacc accagaccca
    33781 tgcgtcgcgc taccaggcgc cggaaaccgc cagcgtggag tacctggtgc tggacctgga
    33841 gagcctgaag aaaagcgtca gcgtgagcga gcaggacctg cgcacctact acgagcagaa
    33901 cgccgccagc ttcggtacgc tggagacgcg gcgcgccagc cacattctgc tgagcgcggc
    33961 gcgcgacgca ccggccgccg agcgcgaaaa ggccaaggcg gcggccaccg ccctgctggc
    34021 gcagctgcgc caggcgcccg acagctttgc cgagctggcg cgcaagtcct ctcaggacga
    34081 cgccagcgcc gccagcggcg gcgatctggg gcgcttcgag cgcaacaagg gcatggatcc
    34141 ggccatcgcc caggccgcgt tcgggctggc caaggtgggc gacatgagcg aagtcgtgga
    34201 aagcgatttc ggctttcaca tcgtacggct gaccgagatc aaggcggccg aggtgccgcc
    34261 gttcgagaac atgcgggccc agctggaaga ccagctgcgc acgcgcctgg cgcagcagca
    34321 gttcagtgaa atggccgacg agttccgcaa cctggtgtac gaacagtcgg acagcctcaa
    34381 gcccgcggcc gacaagctgc acctgaccat ccagacggcc gacaagctgt cgcccacgcc
    34441 ggcgccgggc gccagcgggc cgctggccaa tccgaagttc ctaggcgccc tgtttgccgc
    34501 cgacgccgtc gacaagaagc gcaacaccga ggccatcgac ctcggcggca accagatcgc
    34561 ctccggccgc atcgtgtcgc acaccccggc gcatgcgcgc gccttcgacg aagtcaggac
    34621 cgaggtgcgt caggccttcg tgcgtgaacg cggcgccgaa ctggcgcgcc aggaaggtca
    34681 ggcccgtctg aaggcctgga cggcgcagcc ggcatcggcc acctcactgg ggtcggcgtt
    34741 ggtgctgtcg cgcgacgaac cccagggcca gccgatggcg ctggtggagg cagtgctgcg
    34801 ggccgacccc gccaagctgc ccggtttcgt cggcgtcgac ctcggggcgg aagggtttgc
    34861 cgtcgcacgt gtcgacaagg tgctggctgc gccgacccag agcgccgagc agacggccca
    34921 gagccgggcg cgctacgacc agctgtgggg tctggccgag gcccgcagct actacgagtt
    34981 gctcaaggcg aagtacaagg cccagatcct agtcccggcc cccgccccga cggcgcgttg
    35041 actcgcggcc cagtcgaccc atgcatgaaa agcgccatcg gcgctttttt tgcgaccgag
    35101 cacgggcggg ccttgtgcgt caggctcagc cggatcgaag gcgggagcat cgttgcccgc
    35161 ggcgctgcag gtgtcgcgcg ctcagtggcg atcggtcagc ctaggaggct gtcgggcttg
    35221 gagcgccgaa agcgaagatc ggccccagcg agaccagttt ttgccggatt tgcagcccaa
    35281 tagccgagca attggacaaa aagccggtgc tctcgaagag cggcgaagcc aaaaatgggc
    35341 cgctgcggtc gatttgcagc cggcgatttc caagtccgac agcctcctag ggtcagaggc
    35401 cagaaaaagc ccatgaccct ttgtgcaggg ccatgggcgc cgattcggtg gggtggctga
    35461 tgggactcga acccacgacg acaggaatca caatcctgga ctctaccaac tgagctacag
    35521 ccaccactga gtaccgttga ttataggaca tacggtatgc gcgcgtggct ggccttcggg
    35581 gtgagatcct cccatcctgc gtcgatccgc tctagactgg cgctgccacc atcaagcgga
    35641 gacacagaat gctgaaatcc gatcacctga acgacgccca ggcgctgctg aacctgaacg
    35701 atctgccggc ggcgccgtcg cgccggcgcg cgctgaggct gggcgttggc atgggctacg
    35761 ccgcggccgc cctgccggtg atggcgcaga ccgccatccg cacccccacc gacggcctga
    35821 ccgtgggcga ggtgaacatc gacgtggccg gcttcagcat gccggccttt cgcgcccagc
    35881 cggccggcag gagcgggctg ccggtggtgc tggtgctgtc ggaaatcttc ggcgtgcacg
    35941 agtacatcgc cgacaccgcc gtgcgtctgg cgcgtgccgg ttacctggcg atcgcgcccg
    36001 agttgttcgt gcgccagggc gacgcgcaaa gctatggcga gatggccaag ctgatgagcg
    36061 aggtgattgc caaggtgccc gatgctcagg tgatgggcga tctggacgcc accgtggcct
    36121 gggccgcgca gcatggcggc aactccgaca aactggccgt caccggtttc tgctggggcg
    36181 ggcgccagac ctggctgttt gccgcccatc accccaaggt caaggccggc gtggcctggt
    36241 atgggcgcct ggtcggcacc gccacgccgc tgacgccccg ccacccggtg gatgtggcgg
    36301 cgcagctgaa ggcgccggtg ctgggcctgt atggcggcgc cgacaccggc atcccgctgg
    36361 acacggtcga gcagatgaag gccgcgctgg cggccgccgg cacgcagggc aacgctgcgg
    36421 ccaggggttc ggcgttcgtg gtctacccgg atgcgccgca cgccttccat gccgactacc
    36481 gcccgagctt tcgcaaggaa gcggccgagg acggctggaa gcgcgcgctg gcctggttca
    36541 agcagcacgg cgtggcctga ggtgcccgag gcgatcgccg gcgccctgcc ttgcgcgcca
    36601 ccgcaggcgc acgcatcgtg ggcctggtcg gctggcagca gggtgtgcgg tggatcggcg
    36661 ccgggcagaa cggaaaaggc ttgcgaggcg cgtcaggcaa gcgccagacg ctattgataa
    36721 tatagtgcag agtttcgcgc catatggaac atcaaaccgt gtcttttcgc ccatgatgtc
    36781 tcgttgcaaa tccgcgccat agcggagctg ctgctgtggt ttgcgcctca tcctggccag
    36841 caatccaccg gttttgtttg ttccatccag cacgcaacgc cacaagagcc atcgggcttc
    36901 agccgcgtgc caccggcagc cctggggtgc ggcaccactc gctccagctg ccggcgtaca
    36961 gcgccgccgg ggcatagccg gccagctcca tggccagcag gttgggcacg gcgctgacgc
    37021 cgctgccgca gtggtgcacc acgccggccg ggtcgcggcc ggccagcagg gcgtcgaatt
    37081 cggcgcgcag ctgggccggc gccttgaagc agccgcgccc atccaggttg tcgacgtagg
    37141 ggcggttgag cgcgccgggg atgtggccgg ccaccggatc gagcggctcc tcgtcaccgc
    37201 gaaaacgcgc ggcggcgcgg gcgtcgatca cggtctgccc gggctggccc agcccggcct
    37261 gcacctcgtg aacgtgcagc aggcggcgcc gcggtggctg cagctggaac cgggtggcgg
    37321 ggtgctcggc cgccgggccg gaggccaccg cgccgcccgc cgccagccag gcctgcaggc
    37381 cgccgtccag caccgccacc gcgtcgtgac ccgcccactt gaacatccac cacaggcggc
    37441 cgcagtagtt gcagccgttg cggtcgtaca ccaccgcctg catggggttg gcaaagccga
    37501 ccgcggacag ccaggcggca aagcgctcac gcgggggcag cgggtggcgc ccgccgttga
    37561 tggcctcggc cggcgggtgg gcgctgaggt cgcgctccag atgcgcctga cggctgccgg
    37621 cgatgtgctg ttcggcaaac atggcgtcgg cccgggccgg gttgcccagt tcgccgctgc
    37681 agtcgaacac catcagcggc gcgccgctgg cctgcagcgc ctgaagttcg gcgacggtaa
    37741 tgaggtgggt ataggtcatg ggtcagcctg ggttcagtga ctttcagccg gcgggttggg
    37801 cagggctcgg gcgcgcagca cggtggccgc gatgccgctg gcgacgatgg tggccatgcc
    37861 cagccagccg atcagcggca gctgatcgcc gaacaacagc aggccgaaca gggccgcgaa
    37921 cacgatgccc gagtattgca ggttggccac caccatggtg gcgccgcggg tgtaggcgcg
    37981 tgtcatgcac agctgcccca gcgccgccag gatgccaacc ggcagcagcc acagcgcctg
    38041 cacggtatgg agcggatggg tgtcgaagaa ctgcatgccg atgccacccg ccagcgccgc
    38101 gccgagcgag aaatagaaca ccgtgcgcgc ctccggctcg ccgacgcggc cgagcgccgc
    38161 cacctggatg taggccagcg ccgacatcag gcccgacagc agcccgatca gcccggcaaa
    38221 cagctggttc tgctccagcg tggggcgcag catcatcacc acgccggcaa agccggccat
    38281 caccgccatc accagcgggc cctggcggcg gatgtcggcc agctgcccca tcagcaacgt
    38341 gccgccgacc aggaaggccg ccacccagac gccgctcatg tagttcagcg tcatggcggt
    38401 ggccagcggc aggtgggcga tggcgtagaa ccagcacacc agcgccgaca cgccgaccac
    38461 gttgcgccac acgtgcatca tgggcacgct ggtgtgcagg cgcacgccgc gcgagcggca
    38521 cagcgcccac atgaacagca cgccgatggc gccgcgatag cacacgatct ccagcggccc
    38581 gaagtagggt gaggcgaact tgatgcacac gctcataagg gcgaagaaga gcgaggcggc
    38641 gaccatccag gcggcttgca tggcgtgtga ttatcagaca aaaatgcctg cagcgcttgt
    38701 cccatgtgcg ccagcagcta tcaataaaat agcgcctggg gcgtttgccg ccaggcgccg
    38761 cgggctcagg gtgccgcgcg ctcaggtggt gacgcgcatg cccatggcac gccggtacca
    38821 ctcgtggaag tgctgcatgc cgtcttccat cgggctctgg taggggccga cttcgttgtc
    38881 gccgcgctgc atcagggcct tgcggccggc gtccatgcgc tcggcgatct cgtcgtcctc
    38941 gatcgcggtt tccatgtagg cggcgcgctg ggcctcgacg aactcgcgct cgaagcaggc
    39001 gatctcttcg gggtaataga actccaccat gttcagcgtc ctgtccacgc ccgtcgggtg
    39061 cagggtggag acggtgagca catgcggata ccactcgacc atgatgtgcg ggtagtaggt
    39121 cagccagatc gcgccgcgct cgggcagctt gccctcgcgg tacttcagca gcacctcgtg
    39181 ccattggcgg tacacgtccg agcccggctt gccgaagctg ggcgccaggc ccacggtctg
    39241 caccgagtac tcgcccccaa actgccactt caggtcgtca caggtgacga agttgcccag
    39301 gccggggtgg aaggggccga cgtggtagtc ctccaggtag acctcgatga aggtcttcca
    39361 gttgtagttg cactcgtgca gttcgacgtg atcgagcacg tagccgtcga acgacaggtc
    39421 cttggcggtg gtcatgccgg ccaggtcggc ggcgatgtcg cggccgttgt catcgaacag
    39481 cagaccgttc cactggcgca gcgggtagtt gctgaggttc aggcaggggt cgcgctcgaa
    39541 gtgcggtgcg cccagcagct cgccgcggct ggaataggtc cagcggtgga tggggcagac
    39601 gatgttgccg cccgcatggc ccttgccttc ggccagcagg ttgccgcgcc cgcccagcat
    39661 gatggcctgg cggtggcggc agcagttgga gaccagctgg atctggccct gcgcgtcgcg
    39721 cagcagcgcg cgcccgccgg cttcctgcgg cagcacgaaa tagtcgtggg cttcgggtac
    39781 cgccagcgcg tgccccacgt agcgcgggcc ggactcgaag agcagcttct tttcgcgctc
    39841 gtacagcgcc ggatcgaaat aggaatggac gggaagttga cttgccgcct gctgtaactg
    39901 aagactcaaa tcagacatgg tgtgccgatg ttgaagactc cccctatgaa ggggcaggaa
    39961 gtaagcgcct ggcatccgag gatattgcgc gcgcgaaccc gcgcgccacg cgcgccgggg
    40021 aaccttttat tttagccgcc ttgccgcggc cggccccggg ctggccccgg gccgatcggt
    40081 cggtgggccg gtggcctggg cgacgggcgg cccgcgcctt gccgccgccg cctaaaatag
    40141 gcgcttttgt tcgcgcacat gagccgaaaa gccgcttccg ctccggccca gcctgccagc
    40201 tatggcgagg cggtgcagca gcttgaacaa ctgatcgccg ggctcgaatc cggccagctg
    40261 ccgctggaag atctgctgag ccattaccag cgtggcacgg agctgctgcg ctactgccgc
    40321 gaccgcctgc aggccgtcga agaccaggtg cgcgtgctgg acgagcgcgg tgacgtgaaa
    40381 ccgtggatgc cggagtgaac gcgcgcgatt tcccgacctg gtccgacagg ctgctgacgc
    40441 gcgtcgagca ggcgctggtg gactgggtac cggtcgatgc gcccgccggc ctgggcgagg
    40501 ccatgcgcta cgccgtgctc gacggcggca agcgcctgcg gccgctgctg gtgctggccg
    40561 ccgccgaggc ggtgcagggg cacgccgcag cggcgctgcg cgcggcctgc gcggtggaat
    40621 tgatccacgc ctactccctg gtgcacgacg acatgccatg catggacaac gacgtgcttc
    40681 gccgtggcaa gccgacggtg catgtgcgct tcggccaggc ccaggccctg ctggccggcg
    40741 acgccttgca ggcgctggcg ttcgagctgc tgacccccga gggcgatgag gtgccggcct
    40801 ccatgcaggc ggcactgtgt ggcctgctgg cgcgcgccgc cggtgcgacc ggcatggcgg
    40861 gaggtcaggc catcgacctg gacagcgtgg ggcgcgtgct gaccgaggcg cagctgcagg
    40921 ccatgcaccg gctgaagacc ggcgccctgc tgcaggccgc cgtgcacatg ggcgcggcct
    40981 gcgccacgcc ggcgctgccg gcccacaccc gggccgcact ggaccagtac ggcgcggcgc
    41041 ttggtctggc ctttcaggtg gtcgacgaca tcctcgacgt caccgccgat tccgcccaac
    41101 tgggcaagac cgccggcaag gatgccgcgg ccgacaagcc cacctacgtg tccctgctgg
    41161 gactgaccca ggcccaggcc ctggcggcgc agttgcacac ccaggcgcac caggcgctcg
    41221 acgccagcgg cctgactgcg ctggcgccgt tgcgctggct ggccgaccgc gtggtcaagc
    41281 gcgagaactg acgatgtccc aagccctgct cgaatccatc cacagcccgg ccgatctgcg
    41341 ccgcctgccg cgcgcgcagc tcaagggcct ggcggacgaa ctgcgcgcat gcgtgctgac
    41401 caacgtggcc cgcaccggcg gccacctgag ctccaacctg ggcacggtgg agctcaccat
    41461 tgcgctgcac tacgtgtttc agacgccaca cgaccggctg gtgtgggacg tcggccacca
    41521 aacctatccg cacaagatcc tgaccgggcg acgcgagcgc atgcccacgt tgcgtcagct
    41581 gggcggcatg tccggctttc cgcgccgcga cgaaagcgag tacgacacct tcggcaccgc
    41641 gcattcgtcc accagcatct cggccgcgct gggcatggcg ctggcggcgc gacagaaggg
    41701 cgaggaccgc cacgcggtgg ccatcatcgg cgacggcgcc atgaccgccg gcatggcctt
    41761 cgaggcgctc aacaacgccg gcgtcgagcg tgacgcccgc ctgctggtgg tgctcaacga
    41821 caacgacatg tccatcagcc cgccggtggg cgcgctcaac cgctacctgg cgcagctgat
    41881 gagcggccac ttctatgccg ccgccaggag cgtgggcaag acggtgctgg gcacggtgcc
    41941 gccgctgttc gagctggccc ggcggctgga gcagggcgcc aagggcatgg tggtgccggc
    42001 cacgctgttc gagaagttcg gcttcaacta catcggtccg atcgacggcc acgatctgga
    42061 ttcgctgatc ccgacgctgg aaaacatccg cgacctgatg cgcgacaagg ccgggccgca
    42121 gttcctgcac gtggtcacca aaaagggcca gggctacaag ctggccgagg ccgatccggt
    42181 ggcctaccac ggcccaggca agttcgatcc ggcggtgggc ctggtcaagc ccagcgcccc
    42241 ggcagcgccc accttcaccc aggtgttcgg gcagtggctg tgcgacatgg cggcgcacga
    42301 ccgcaagctg gtgggcatca ccccggccat gcgcgaaggt tcgggcatgg tcgaattcca
    42361 caagcgcttt ccggaccgct actacgacgt cggcattgcc gagcagcatg ccgtcacctt
    42421 tgccgccggt ctggcctgcg agggcctcaa gccggtggtg gccatctatt ccaccttcct
    42481 gcagcgcgcc tacgaccagc tgatccacga cgtcgccatc cagaacctgc cggtggtgtt
    42541 cgcgctggat cgcgcgggcc tggtcggtgc cgatggcgcc acccatgccg gcaactacga
    42601 catcgccttc ctgcgctgcg tgcccaacgt cagcctggcc tgcccggccg acgaacgcga
    42661 gtgccgtcag ttgctgacca cggcctacga gcagagccat ccggtggcgg tgcgctaccc
    42721 gcgtggtgcc ggagccggcg tggcaccgct ggctcagctg accgggctgc cgttcggcaa
    42781 gggcgaagtc cggcgccagg gcaagggggt ggccatcctg gcgttcggca cgctgctgta
    42841 ccccgcgctt gccgccgccg aacggctgga cgccacggtg gtcaacatgc gctgggtcaa
    42901 gccgctggac gccgagctgc tgctgcaggt ggcagccagc cacgacgcgc tggtgacgat
    42961 cgaggagggc gccatcatgg gtggcgccgg cagcgccgtg gccgaggcgc tggccgcggc
    43021 cggggtggtc aagccgctgc tgcacctggg cctgcctgac cgcttcatcg agcacggcga
    43081 tccggcgcga ctgctggcgc tcgagggcct ggatgccact ggcatcgagg gttcggtgcg
    43141 ctcgcacttg ggctcgtggc tgatgccgcc tcagcgcaac ctgaaagcgg tcagctgacc
    43201 attcaggcgc gccacggcgc ccggcgatcc gatcaagtga atgccctaat ggctggcgcg
    43261 gtttacaagc cgctacactc gaccggttgt tctttttttg aggagtacgc aacatggatc
    43321 gtcgttccct gatcaagcgt gccggcatcg ccggggtgct ggctgccggc gtggcgccgg
    43381 cggtgcatgc gcaggcggcc gtgcgttggc gcatagccac cagctttccg aagtcgctgg
    43441 agaccctgca tggctgcgcc gtgaagttct ccgaaaccgt caaggccctg tcgggcggca
    43501 agttcgaggt ctccgtgcat gctgcgggcg agctggtgcc ggcctttggc atcgtcgacg
    43561 ccctggccga cgattcgctg gaaatgggcg agacggcctc gtactactac accggcaagc
    43621 acccggcatt tgccttcggc tgcgccgtgc cgtttggttt caccgcccag cagatgcagg
    43681 cctggaagcg ccacggtaac ggacgcaagc tgatggacga gttgtacgac aagtacaact
    43741 tcttcggcct gagcgccctc agcaccggca cccagatggg cggctggtac cgcaaggaaa
    43801 tgaagacccc ggccgacttc aagggtctga agatgcgcct gggtggcggc gtgttcggcg
    43861 agtccatggc caagctgggc gtggtggcgc agaacatgcc ggtgggcgag gtgtaccagg
    43921 ccctggaaaa gggcacgctc gatgccgtgg agttcgtcgg cccctacgac gacgagaagc
    43981 tgggtttcaa caaggtcgcc aagtactact actacggcgg ctggtgggaa ggcagcgcgg
    44041 agctggagtt cttcatcaac aagaaggcgt ttgccaagct ctcgcccgag aaccaggcca
    44101 tcgttcgcgc cgctgccgac gtggcttatg ccgatggcac ctccaagtac atgcacctca
    44161 accccatctc gctcaagcgc ctggtgggct cgggcacgca gctcaagcgc tttcccaaac
    44221 ccgtggtgga agccggcttc aaggccgcgc aggcggtcta tcaggagcac agcggcaagg
    44281 acgccctgtt caagaagatc tacgaagaca tgcgcgcctt ccagcgcgac cagatcctct
    44341 ggaaccgcat ctccgagctg gcgttcaccc aggacatgag cacgctgaag atctgagtgt
    44401 cagggtctgg cgcaccccgg tgtacttgcc ggggggcaca aaaaaaaccg cagcctgtca
    44461 agggctgcgg ttttttcatg ccgctcagga tgacggcttg ccggccgcgc gctccagagc
    44521 cttcacgggg tcgtcgtcct cttggccctg gttgccctcc tggcctgcct ctggtgagga
    44581 cgggtcggcg ggagcgtcca ggccttcgat ggcgggtgat tcttcgctgc tgctgcccgg
    44641 ctcctgggta ccgatgccgt cttcccggcc ctcgcccaat ccttcggcct cgctgtcctg
    44701 gttgagctgc tcgaacatct gctcgcccac ggccttcatg tccacgtcga ccttcttgtc
    44761 caggttgctg gtcaccaggc cggggaaggc gatcaggatg gccaccatgg tcagctgcag
    44821 caccaggaag gggatggcgc ccttgtagat ctgcatggtg gtcacgggct cgatggtctt
    44881 gcccgtcaca tggtccacat agggcttctc gggtgcgacc gagcgcagga agaacagcgc
    44941 aaaaccaaac ggcgggtgca tgaaggaggt ctgcatgttc accgccagca gcaccccgaa
    45001 ccagatcagg tcgatgccca tcttgtcggc aatcggcgcg agcagcggga cgacgatgaa
    45061 ggacagctcg aagaagtcca ggaagaacgc caacaggaag atcatcacgt tgacgaagat
    45121 cagaaagccg atctgccctc cgggcagctt ggccatcagg tgctcgaccc acaccggccc
    45181 gtcggccgcc tggaacacca tgctgaagat ggtggcgccg atcaggatga acatcacgaa
    45241 cgacgacagc ttggtggtcg tgcccagtgc ctgcttgagc aggtccatgt tgatgcgccg
    45301 gcgcatggca gccatgatca gtgcgcccat ggcgcccatg gcgccgcctt cggtgggggt
    45361 ggccacgccc aggaagatgg tgcccagcac caggaagatg agcagcagcg gcggaatcag
    45421 cacaaaggtc acgcgctcgg ccagttgcga cagcagcccc atgcgcgtga tgcggttgac
    45481 gagcgcgatc agccaggcga cgaaggtgcc gccgcacatg gcgacgacga tcttttcgtc
    45541 ggtgggcacg aactcgacgg gcgtgccctg ccaccaggtg tgcacggcct ccatgttcct
    45601 ggccagcacc acggccacgg ccgtggacag cagggccagc acgcccagcg agcggtagcc
    45661 cgaatggcca ttgggctcgc ggaaggtgcg ggcctcgggc ggcagggccg gcaccatgct
    45721 gggcttgaag atggcgagcg caaacaccca caggatgtac aggcccatga gcgcaaagcc
    45781 ggggatgaag gcgcccttgt acatgtcgcc cacgctcttg cccatctggt cggccagcac
    45841 gatcagcacc agggatggcg ggatgatctg cgccagcgtg cccgaggcgg cgatcacgcc
    45901 cgaggccagg cgtcggtcat agccatagcg cagcatgatg ggcatggaga tcaggcccat
    45961 ggagatcacc gatgcggcca ccacgccggt ggtggccgcc agcatggcgc ctaccagcac
    46021 cacggccagc gccaggccgc cgcgcagcgg gccgaacacc tggcccacgg tctcgagcag
    46081 atcctcggcc atgccgctgc gctccagaat cagccccatg agggtgaaga agggcacggc
    46141 cagcagggtc tcgttgctca tgatgcctat gaggcgctgc ggcagccagg aatagatagc
    46201 gctggtgaac acccccagct ccacgcccac cagggcaaag aacaggcccg ccgcgcccag
    46261 gctgaaggcc acgggaaagc ccagaatcag gaacacgatc agcccggcga acatcaccgg
    46321 ggcaaagttg gcagcgatga attccatggt gcagatgctc cgcgtgcgcg tcagtgggtc
    46381 tggctggcgc tctgctgctg gcgcagcgcg tccgcgagct cctgctcggc ggtcttgtcc
    46441 agggccttca cggtcggatc cggcccctgg ccgcgcagga aggcgatgca cttgatcagc
    46501 tccgaccagc cctgcagcag cagcagcacg aagccgatcg gcagcgccgc ccacaccggc
    46561 cagcggatca ggccgccggg gttgggtgac atctcgccgg aggcgatccg ttcgaacacg
    46621 atcggcgtgc tcatccacag cacgatgacg cagaccggcg tcaggaacag cacatagccc
    46681 aggatctcga tccacagctg caccttcttg ggcaggcggc tgttgagcac gtcgatgcgc
    46741 acatgctcgc ggtgcagcag cgtgtagccc gcggcggcca gaaacgacca ggcgaacaga
    46801 taccactgca cctcgaggaa ggcgttggag ctggtgtcga aggccttgcg gaccacagcg
    46861 ttcagcgcgc tgatgatggt ggcaccgaag atcagccaga tgacgaaacg accgagcgag
    46921 gcattgagcc agtcgacggc acgcgaaaac ttgagcagcg cttgcatcgg gatctccgtg
    46981 agggggcgtg gtgcgcaggg tgggcgcgac caggggcggc ggtaaccggg tgggccatgc
    47041 cgcagacaga tcgcaggcga ttgtaatgag gcccctgggg cggcgccgta tagcatcggc
    47101 acggtctgcg ccattcgaca ccatgagcac cctggccgaa agcgcgcgcg ccgcgctgca
    47161 actcatcgct tcgctggacc cgttgctgtg gtcggtggtg ggccgatcgc tggcggtcag
    47221 cgggctggcc tgcctgctgg cctgtggggt cgggctgctg gccggtgcgt cgctcgcggt
    47281 ggcgcgcttt cgcgggcgcg aggccgtgct gacgctgctg cacaccctgc tggccctgcc
    47341 gtcggtggtg gtcgggctgc tggtgtatct gctgctgtcg cgcagcggcc cgctgggttt
    47401 tctgggttgg ctgttcagct ttcaggccat ggtgctggcg caggcggtgc tggtggtgcc
    47461 ggtgatcgcc gcgctcacgc gccagacggt ggaagacgcc gacctccggc atggcgagca
    47521 gctggcgtcg ctgggtgccg gcccttgcct gcgcgggttg ctgctggcct gggacgagcg
    47581 ctgcgccctg ctgatggtgc tgatggcggc gtttggccgg gcggtgtcgg aggtgggcgc
    47641 agtgatgatc gtcggcggca acatcgacgg cttcacgcgg gtgatgacca ccgccatcgc
    47701 gctggagacc agcaagggcg atctgccgct ggcgctgggc ctgggcatgg tgctgctgtc
    47761 ggtggtgctg ctgctcaacg ccgccatggc cggcctgcgc cattggcgag cgcgcaccga
    47821 cgctctggcc cccggcgcgg tgcagggacg gtgggccgca tgagccgacc cgccggcccc
    47881 ccgggcgcag cggcgccgct gatccgcctg caggatgtgg gcgtgcgctt cggccgggtg
    47941 caggcgctgc agggcgtggg gctggagatc gccgccggcg agtgcgtggc cctgatcggc
    48001 ggcaacggca gcggcaagag cacgctgctg cgggtgatcc acggtctgat cgcgccgacc
    48061 gagggccagc tggtggtgcc ggcgcgccgg gcgcaggcga tgctgtttca gcgcccctgg
    48121 atgatgcgca ccacggtgca gcgcaacgtc gcgctcggac tgtggctgcg cggcctgccg
    48181 tggcgccagg cgcaggagcg cgcgttgggg gcgctggagc gcgtgcaact ggccgaactg
    48241 gcgtcgcgcc gcgcgcccgg cctgtcgggc gggcagcagc agcgcgtggc gctggcgcgt
    48301 gcctgggcgc agcaggcgca actgctgttg ctggacgagc ccacggccag cctggacccg
    48361 cacgccaggc tcgaggtcga ggcgctgatg cgcgcgttca ccggcacggc gcccgacgcg
    48421 gcgccgttga ccatggtgtt cgccagccac cacctgggcc aggtcaggcg cctggcgcag
    48481 cgggtcatct gtctggacca ggggcgcgtg ctggccgatc tgcctgtggc acagttcttc
    48541 gaccacgcgc ggctgagctc ggtggcgccg gtggcggcgg ccttcgtgca aggagaaatg
    48601 tgatgcaaaa attcagaaac ttttggcctc tggcgctttt gcagactgcg ctggcagcta
    48661 tcttaacgat agcatttccg gtggcctcgg cgcaaaccat cgtgatggcc tcgaccacct
    48721 cgaccgaaca gtcggggttg tttgcccacc tgctgccgca gttccgccag gccagcggca
    48781 tcgacgtcaa ggtggtggcg gtgggcaccg ggcaggcgct ggacatcgcc cggcgcggcg
    48841 acgccgatgt gctgttcgtg catgacccga aggccgagga gcagttcgtc accgacggct
    48901 ggggcctcaa gcgccacccg gtcatgtaca acgacttcgt cctggtcggc ccgcgcagcg
    48961 acccggccgg ggcgcgcggc cgcgacatcg tgcaggccat gcggcagttg gcgggggcgc
    49021 acgcgccctt catttcgcgc ggcgacaaaa gcggcaccca cagcgccgaa ctgcgcttct
    49081 gggcccaggc gggcctggcc gaagcgcgcg ggcgtggcta tcgcgaatgc ggttgcggca
    49141 tggggccggc gctgaacatg gctgccgcca gcggcgccta cgtgctggcc gaccggggca
    49201 cttggctcaa cttcaggaac cgggccgacc tggcaatcct ggtcgaaggc gacccgcgcc
    49261 tgttcaatcc ctacggcgtg ctggtggtca acccggcacg tcacccacag gtcaaggccg
    49321 ccgaggcaca gaagtttgtc gactgggtga cgtcgcccgc cggtcaggca gccatcgcca
    49381 gctaccgcat cgggggccag gcgttgtttt tcccggacgc gcgaccttga ggcggcgtct
    49441 gaccccctga acaggtgact ggactcgcag aaaatgaaat aataagtatc cagttacact
    49501 ttcaggaggg ttggtcatgg catgtttccc ttcctggcgg acgctctgcc accatgcgtt
    49561 gggcgttgcg cttggcctgc tgctgctgcc ggcgcaggcc cagatccggg tgtcgatcaa
    49621 tccgggcgac cagatcgagg ccgatcgcgt gggcatcttc aacgcctgga agggcgcgct
    49681 ggatgccggg ctgcgcaagg ccggcgccag cgcggcgcag gcccgctttt ccagcgacgc
    49741 cacggccgac atgaatgccg gccgcgccaa cgtgttcgac ctgatggtgg ggccggcgcc
    49801 ggtcatcggc agcgccgtgc gctacggcta cgtgccggtg gtcggcgcca cccagaaggc
    49861 gcgcgccgtg ctggtggcgg ccgagggcag cgccatcgcc gactgggccc agagcaaggg
    49921 caagcgcctg ggcatgccgg gccaggacag cgtggtgacc tatctgctgc gcggcgaggt
    49981 gcaggccggc aacaccacgc tggcacgcca gtacccgtcg atctaccaga cacgctacca
    50041 ggacgccctg ctgacctgcc tggagctgcg ccgctgcgac gtggtggcgg tggaggaagt
    50101 cgtggccaag cgctgggcgg cgagcggcaa gaaggtcaag gtggtctggc agagccgcga
    50161 ggtgccgggt ctgagcgtgg cgctgcacaa ggacgcccag gtgtcggccg acaccctgcg
    50221 cagcgcactc atggccgagc tggccaggct cgatgccttt ggctacaccg gcacgttgac
    50281 ggtcaaggat ttcgagtacg tgtccacgct gggctacttc acgccgcgca ccctggaagg
    50341 cgcgaccctg atcgaagacc cggcgcaggt ggagtcgctg ctggccgctg gcgcgcgcta
    50401 tatcgacgca cgcaaccagg aagagttcgc cgccggccat gtgccgggcg ccatcttggt
    50461 gccgtacgtc gaaaagagcg ccaaggatcc gggctacgac cccaagctgg atcagttcga
    50521 cctccagcgc ctgggcgaca acaaggcgca gccgctggtg ttcggctgca acggcccgga
    50581 atgctggaag agcttcaagg ccagccgcgc cgccctgcag gccggctaca ccaaggtgta
    50641 ctggttccgc accggtgtgc cggcctggcg cagctcgggc cgcccgctcg atcgtggtga
    50701 cgccaaggcg ggctgaacgg ggcgtcagtc gagcaccgcc acgcccttga tctgggcata
    50761 caccggctgc ccgggcgcca gcgccagcgc ctcggccgac ttgcgcgtga cgcgcgccag
    50821 cagcggtgtg gcgccgaggt ccagcgccac cagcacctgc gccgggccgt cttcggccag
    50881 cgaacgcacg cgggccggca gcacgttgag aatgctggtg tcgtgcgcgg cggacaggca
    50941 caggctgacg tcacgcgcct gcacccgcag gcgcacgcgc tggcccggcg tgcgcggcgg
    51001 ggtgccggcg ctgtgcacgc agtgcaggac accgtgatcg aacgccacct gcagcaactg
    51061 gtagtgcggg tcgaagctca gcacctgtgc gctcaccagc gcgctggcgg catcgccctg
    51121 ggtgcgcgcc acgtccgggc gcgtcagcag cgcctcgacc gggccgtgcg cgacgacctc
    51181 gccatcgcgc agcagcacca ggtggtcggc caggcggatc acttcgtcga gcgcatgggt
    51241 gacgtacagc atcggcaggc gcagctcgcg cgtcagccgc tcgaagtagg gcagcagttc
    51301 ggccttgcgt gcggcatcca gcgaggccag cggctcgtcc atcagcagca ggcgcgggct
    51361 ggtggccagc gcgcgcgcga tcgccacccg ctggcgctcg ccgcccgaca gctgcgccgg
    51421 gcggcgtgcc agcatggggc ccaggcccag cagctcgatc gcctgcggca gcgccacgcg
    51481 ccgctcggcg gcgggcacgc gctgcagacc gaagtccagg ttgccctgca ccgtcaggtg
    51541 ctcgaacagg ctggcttcct gaaacaccag gcccagcggg cgccggtggg tggggcgcca
    51601 gatgcggtgc gcgtcgtcct gccacacgtc gccgcccacc gccacgcgcc ccggtgacgg
    51661 ccgcgtcagc ccggccacgg cgcgcagcag cgtggtcttg ccacagcccg aggggccgaa
    51721 cagcgccgtc acgccctggc ctggcaggcg cagctcaacc ccgagccgaa agccgctgcc
    51781 catgcgcacc tgggcgcgca gttcgatgtc gtggccggtg ccctgcgtca tgccggcgcc
    51841 ttgcgcgatt gcgtcaggtg cagggtcagc agcaccagga agctgaacac cagcatgccg
    51901 ccggccagcc agtgcgcctg ggtgtattcc agcgcctcga catggtcgta gatctgcacc
    51961 gagatgacgc gcgtctggcc ggggatgttg ccgccgatca tcagcaccac gccaaattcg
    52021 cccagggtgt gggcgaagcc catcacggcg ccggtcaggt agcccggccg cgccagcggc
    52081 agcgccacat gcaggaacgc atccagcggg ccggcgcgca gcgtggccgc ggcctccagt
    52141 gggcggcggc cgatggcttc gaaggcgttg accagcggct gcaccacgaa gggcagcgaa
    52201 tacaccagcg aacccagcaa caggccccag aagctgaacg ccagcgtgcc ccagcccagc
    52261 gcctgcgtca ggcggcccag cggaccctgt ggccccagca tgaccagcag ataaaacccc
    52321 agcaccgatg gtggcagcac cagtggcatg gccaccagcg tgcgcaccgg cgtgcgcaac
    52381 caggccagcg cgcccgcgtc gccgcgcgcc agccaccacg ccaggggcgt gcccagcacc
    52441 agcaggatgc aggtggtggc cagcgccagc tcgatcgaga gccggacggc ggcaaggctg
    52501 gtggcgtcaa gcatggggca aagcaaatgc tcaggcggcg ccggggcgcc gccgtaactc
    52561 atgttttgat agctgttggc gcttgatgtg caagcgccga aggcccattt tgccttgaag
    52621 ggcttacttc acggcgtagc cgtagctgcg gatcaggtcc ttggtgcgcg gcgagctcag
    52681 cagcgccatc caggctttgg ccgcggggtt gccctgtccg cgcttgagga tcacggcgtc
    52741 ctgcacgatg ggcgcgtgca gcgagcccgg caccacccac atcgaaccac ccttgagccg
    52801 cccaccctgc agcacctgcg acatggccac gaagcccagg tcggcgttgc cggtggcgac
    52861 gaagttgtag gtctgaccga tgctctcgcc ggtcaccagc ttgggctgga gggtcgcttg
    52921 cagacccagc cggttcatgg cctcgacggc ggccgcgccg tagggcgcat tcttggggtt
    52981 ggcgatcgcc agcttgttga actggccgcc ttggagcacc tcgcccttgt cgtccaccag
    53041 gccgggtttg gccgaccaca acaccaactg accggtggcg taggtgaagc gcgagccggg
    53101 gacggtgtcg ccttcctttt ccagccgcgc ggggcgctcg tcgtcggccg acaggaacac
    53161 gtcgaacggc gcgccgttct tgatctgcgt gtagaacgcc ccgctggcgc cgaacgacag
    53221 cctggcctcg tgtccggtgg tcttttccag aatggcggcc agcgccttgg ccggttcggt
    53281 gaagttggcc gccacggcga cgtggaccgt ttcggcgtgc gcggaccacg aggccaccat
    53341 cacggcggcc agggtcacaa gggtacggat gggacgcatg ggggaactct cctggaagcc
    53401 ggcgcggcgg cgccggtagc gttattcaac ttcgggacaa cgagtctaac tcagggaacg
    53461 aagggatcgg cgaactcggg gcgcgcaagc atgcggtcgt ccgtcagcgc acgcaggaag
    53521 gccaccaagt cggcctggtc ctgcgcgtcg atgtcgatct gatcgatccg cggatccttg
    53581 tacgggttgg cgcggccgtc gccttcgtac gggccgttgc cgagcacgcg gccgccggcg
    53641 gcatgcagcg ccaccaccgc ttccagcgtg gcgacgctgc cgtcgtgcag gtagggtgcg
    53701 gtcagcgcga cgttgcgcag gctgggcgcg cggaacctgc ccatgtcctc gggcctgccg
    53761 gtgaattcga gcaaaccccg gttgttggtc gggtaagcac cggtgccgcc gatgttgaac
    53821 aggccgatgt tgtagaaccc cggctcgccc acgcggccct gggcgctgac gaacgcaccg
    53881 ccgcccaggt tggggccgct gtggcactgc gcgcagagcg cgcgctcgcc gaagaacagg
    53941 cgctggccgc gctgctcgct ttcagtcagc gtggccgtgc cggccagcgc ctggtcgtag
    54001 cggctgccgg ccgccaccag cgtgcgctgg aaggcggcga tggccttgat cacgttgtcc
    54061 cagtggatgg gcatcgcttc gccgccaaag gcttgggcga aacgcggcgg gtagcgtgcg
    54121 tcgtccttca ggcgctgcac gatggcgtcg cggttggtgt cgttcacacc catctcgacc
    54181 ggctgctcgc cgaacagggg cgtgtgcatc tgctgctcca gcgtgcgcgg cgccgggttg
    54241 ctccagtcca gcgtggcctg gtaggcggcg ttgaccaggc tgggtgcgtt gcgcggg
//
LOCUS       DAOOOG010000003        50726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_473_length_50726_cov_25.067040, whole genome shotgun sequence.
ACCESSION   DAOOOG010000003 DAOOOG010000000
VERSION     DAOOOG010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 50726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50726
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_473_length_50726_cov_25.067040"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /gene="era"
                     /locus_tag="PK242_00490"
     CDS             <1..524
                     /gene="era"
                     /locus_tag="PK242_00490"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOZ92534.1"
                     /translation="HRRAEIAPWLKGMQERHPFAEFVPMSAKNPRDVARLLEICARYL
                     PEQPWWYGEDELTDRSEKFLASETVREKLFRLTGDELPYTSTVVIEQFKEEKSRAHGR
                     LLRIAATIVVEREGHKAMVIGQQGERLKRIGTEARQELEKLLDAKVFLEIWVKVRSGW
                     ADDEARVRSFGYE"
     gene            611..1378
                     /gene="recO"
                     /locus_tag="PK242_00495"
     CDS             611..1378
                     /gene="recO"
                     /locus_tag="PK242_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOZ92535.1"
                     /translation="MKRVVHEPGYVLHHYDWSESSLIVEAFTRAHGRVALVAKGAKRP
                     SSNFRPVLLPLQPLLLSWGGDGEIRTLKAAEWGGGQVMPRGDALLSGFYANELLLRLL
                     AREDAHPRLFDAYAALVHALAAGDAEPLAAAALRAFELLLLRETGHLPALDAQTLTLA
                     PLQASEPYRLVAEGGLRAAHADDAAWLPGQSWRRLAASLADEAPWTATLTVVVGLPGE
                     QRGSLRRQLGELLHYHGGGATLRTRQLLQDAQRLMAT"
     gene            1402..2154
                     /locus_tag="PK242_00500"
     CDS             1402..2154
                     /locus_tag="PK242_00500"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOZ92536.1"
                     /translation="MPTQLSVNVNKVALVRNTRHLGIPSVTRAAQLCLQAGAHGITVH
                     PRPDARHIRADDVHALAALMRGWPGREFNIEGNPTQNLMDFIRAVRPAQATFVPDSED
                     QFTSDHGWDLADAGVAARLRPLIDECQGLGVRVSLFMDPLPELMAAARAVGAERVELY
                     TEPYAAAWGTPEQGRQLQRYAVAAQAAGDAGLGVNAGHDLNLHNLGTFVARVPGLLEV
                     SIGHAFIADALERGYAATTEAYLSCIAQAGRS"
     gene            2213..2620
                     /gene="acpS"
                     /locus_tag="PK242_00505"
     CDS             2213..2620
                     /gene="acpS"
                     /locus_tag="PK242_00505"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOZ92537.1"
                     /translation="MIHGIGTDICDVRRIRASVERHGERFAQKILSEAELAVWRRRSA
                     RWPERGLRYLATRFSAKEAFSKAVGLGMRMPMHWRRCEIANQPSGQPCIVLHGPLKDW
                     FEARHLVAHISVSDESDYAASFCVVERLPPVRE"
     gene            2697..3752
                     /gene="nagZ"
                     /locus_tag="PK242_00510"
     CDS             2697..3752
                     /gene="nagZ"
                     /locus_tag="PK242_00510"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOZ92538.1"
                     /translation="MQHAPLIIDIAGTALTEDDGRRLAHPLVGGLILFARNWQDREQL
                     GSLCAEVRRLRPELLICVDHEGGRVQRFRSGGFTHLPPMRALGELWMQDAMRAQDAAS
                     AYGYVLGAELRACGVDLSFTPVLDLDWGPSGVIGDRAFHADARVVTMLAKSLAHGLRR
                     AGMACCGKHFPGHGFVAADSHTALPRDTRTLKAILAADAAPYGWLSSVLDAVMAAHVV
                     YPRVDARPAGYSARWLQAVLRGRLGFGGAIFTDDLSMQAARHLEGRTVTPVEAVLAAL
                     DAGCDLALLCNQSLGGGAVLDEVIDGLARAQVQGRWQPREASAARCQALLPREPAPDW
                     DALMRSPAYLHALALVP"
     gene            complement(3802..5166)
                     /gene="phoR"
                     /locus_tag="PK242_00515"
     CDS             complement(3802..5166)
                     /gene="phoR"
                     /locus_tag="PK242_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HOZ92539.1"
                     /translation="MVWRVLMFSLLQLACAAAGGLLLGPWGVVGGVVLAAWLAFGWET
                     WSGQRVIRWLRGDGAASSAPVLRGLWGEAAARMRRRLGQQEQQALASGQRLQEFLAAL
                     QASPNGVVLLDPQGHMEWFNQTAAQHFGLDAGRDLQQQIGNLLRDPNFSAYLARPDDA
                     QDLVLNARASTPERPQRLAVRLHPYGEGRRLLLSRDITQLEQAEAMRRDFVANVSHEI
                     RTPLTVLAGFIETMQSLPLSEAERARYLGLMAQQAQRMQHLVQDLLTLSRLEGSPLPG
                     FQTWTPVAALLRPCEDEAHTLSALLTPAGAAPQTLVFPTQDELRAAGELAGDPAELQS
                     ALSNLVTNAVRYTPTGGRITVQWQRLPDGGARFAVHDTGPGIAAEHLPRLTERFYRID
                     RSRSRDTGGTGLGLAIVKHVLQRHDARLHIASVPGQGSVFSVDFPAGRLRDAALARPG
                     AASA"
     gene            complement(5212..5931)
                     /gene="phoB"
                     /locus_tag="PK242_00520"
     CDS             complement(5212..5931)
                     /gene="phoB"
                     /locus_tag="PK242_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HOZ92540.1"
                     /translation="MRKLPTILIVEDEPAIAELISVNLRHNGFAPIWAEDGDSAQREL
                     DAVLPDMILLDWMLPGQSGLELSRKWRGHPRTRAVPIIMLTARGDEPDKIAGLDAGAD
                     DYITKPFSTQEMLARIRAVLRRRAPEHSGESVAIGALSLDPATHRVSHAGQPLKLGPT
                     EFKLLHYLMRNAERVHSRGQLLDKVWGDHVYIEERTVDVHVKRLREALGDAATMVETV
                     RGAGYRLTAQPDASKSIASGA"
     gene            complement(5928..6629)
                     /gene="phoU"
                     /locus_tag="PK242_00525"
     CDS             complement(5928..6629)
                     /gene="phoU"
                     /locus_tag="PK242_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOZ92541.1"
                     /translation="MTDKHLSTQFDSELNQVSARVMELGGLVESQIREAISALSHFSL
                     EAAAQVESIESRVNQMEVDIDHELITIIARRQPTARDLRLLMAFSKATANLERMGDEA
                     ARMARMVRSIIESGAARSLPSSDLRVAADLASGLLRKALDSFARLDVKAAVAILKEDD
                     LIDREFDGFVRKLITYMMEDARTISASLDLLFLAKAIERIGDHSKNIAELIIYLVEGK
                     DVRHRPVADIESTIQ"
     gene            complement(6751..7530)
                     /gene="pstB"
                     /locus_tag="PK242_00530"
     CDS             complement(6751..7530)
                     /gene="pstB"
                     /locus_tag="PK242_00530"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOZ92542.1"
                     /translation="MPPTSHPAVPSKIAVRDLNFYYGKFHALKGINLDIPEKKVTAFI
                     GPSGCGKSTLLRTFNRMFELYPEQRAEGQIVLDGENLLQSKQDVALIRAKVGMVFQKP
                     TPFPMSIYDNIAFGVKLFENLNASDMDERVEWALRKAALWNEVKDKLTQSGAGLSGGQ
                     QQRLCIARGIAVLPEVLLLDEPCSALDPISTAKIEELIAELKNDYTVVIVTHNMQQAA
                     RCSDYTAYMYLGDLVEFGATEQMFFKPERKETEDYITGRFG"
     gene            complement(7555..8445)
                     /gene="pstA"
                     /locus_tag="PK242_00535"
     CDS             complement(7555..8445)
                     /gene="pstA"
                     /locus_tag="PK242_00535"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOZ92543.1"
                     /translation="MNAATTSQKLLSRAALEQARGRRYAARKRVNQVALTLSLAAMAF
                     GVFWLVWILWETLRLGLGGLSLSLFTEMTPPPNEAGGLANALYGSFMMVLLATFVGTP
                     VGILAGIYLAEYGKRGWLAPATRFVNDILLSAPSIVIGLFVYTVVVVRFKSFSGWAGV
                     VALALIVVPVVIRTTENMLLLVPASLREAAYALGTPKWKVITMVTLRAARAGVITGVL
                     LAVARIAGETAPLLFTALNNQFWSGDMSKPLANLPVTIFKFAMSPYENWQQLAWAAVF
                     LITVAVLGLNILARVLTRQK"
     gene            complement(8442..9440)
                     /gene="pstC"
                     /locus_tag="PK242_00540"
     CDS             complement(8442..9440)
                     /gene="pstC"
                     /locus_tag="PK242_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOZ92544.1"
                     /translation="MPQPVRQSGSGQTLPPARGPVLSNSMADRLFAWLARSAALLTLA
                     TLLGILISLVVGAWPSITTYGLGFLTSSAWDPVQNDYGGLVMIYGTLATSAIALFIAV
                     PVSFGIALFLTELSPAWLKRPLGTAIELLAAVPSIVYGMWGLMVFGPLLATWVQQPLQ
                     SLLSGVPWLGALVSGPPVGIGILSAGIILAIMIIPFIASVMRDVFEVTPALLKESAYG
                     LGATTWEVIWKVVLPYTKAGVLGGIMLGLGRALGETMAVTFVIGNMNQLASLSVFEAA
                     NSITSALANEFAEAGEGLHQASLIYLGLVLFFITFVVLSASKLLLSRLQKAEGARA"
     gene            complement(9692..10723)
                     /gene="pstS"
                     /locus_tag="PK242_00545"
     CDS             complement(9692..10723)
                     /gene="pstS"
                     /locus_tag="PK242_00545"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOZ92545.1"
                     /translation="MGSVLAAAVIAPAWAQLEATGAGASFPAPLYSKWAADYHKATGV
                     KINYQSVGSSAGVKQIDAKTVDFGASDAPLTDEELAKKGLLQFPTVVGGVIPVVNIAG
                     IKAGELKLSGAVLGDIYLGKITKWNDPAITALNPGVKLPDATIAPVRRADGSGTTNAF
                     TNYLSQVNPEWKAKVGEGTAVNWPTGAGGKGNEGVAAFVGRLPNSIGYVEYAYVKQNK
                     MTYVQLQNAAGKFVSPGDANFKAAAAGADWSRSFYQVLNNKPGDASWPITSATYILMH
                     KNQDKPAQAATVLKFFEWAYQNGDATADALDYVPMPASVKELIYKSWGQIKDGAGKPV
                     APMAPVALK"
     gene            10950..11351
                     /locus_tag="PK242_00550"
     CDS             10950..11351
                     /locus_tag="PK242_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92546.1"
                     /translation="MFFHSLPPPAPVSGGADGLLARVLGALVWLMLAALGAVFALSLL
                     IWLVVMVVVSLVGSLFTGRPATVTLLWRRYRDMTREVTRSRWSPSPASSTPRADAAQA
                     TGAASAQTSVQDVSWREVPAAPAADGDVPGR"
     gene            complement(11474..13036)
                     /gene="lysS"
                     /locus_tag="PK242_00555"
     CDS             complement(11474..13036)
                     /gene="lysS"
                     /locus_tag="PK242_00555"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOZ92547.1"
                     /translation="MNQPAAPADLQAAASEHDENKLIAERRDKLRAIRAVQAAGGAVA
                     FPNDFKPKDRASQLQQAHGAIEKEVFEADLAAGRQRHASVAGRMMLKRVMGKASFATL
                     QDATGRIQIYVAREAVGEADYEAFKHWDLGDIVACEGHLFKTRTGELSIHATRIRLLT
                     KSLRPLPDKFHGVQDQELKYRQRYVDLIMDEQARARFAARSATLSAMRQFMTGHGFME
                     VETPMLHPIPGGANAKPFVTHHNALDQQMFLRIAPELYLKRLIVGGFERVFEINRSFR
                     NEGISVRHNPEFTMMEFYAAYWNYQDLMDFTEALLRDTAQQATGSLQLSYQGRAVDLS
                     QPFQRMTIRQALHTHTDIGAHVDDAAELIRRLKKLGLSEDKDRLSQRSLASLQVMYFE
                     EEVEQHLWQPTFIMEHPTEISPLARANDQRPEVTERFELYITGREMANGFTELNDAED
                     QAARFAAQASAKDAGDEEAMFYDQDFIRALEYGMPPTGGCGVGIDRFMMLLTDSQSIR
                     DVILFPALRHEA"
     gene            13238..14542
                     /locus_tag="PK242_00560"
     CDS             13238..14542
                     /locus_tag="PK242_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOZ92548.1"
                     /translation="MKAASGSQPSLTRATLTLLGGGALAQLLPLLLGPFIARLFTPEA
                     FGLYTQFATVAAALAVAASLRYEQALPLARSDDEARALLALALRLLLAAVLLGVPLGW
                     ALHALGALPLAVLLAPALATAGLLQVLMLWANRAQRFGALATSRVLHYGGAAALQLVL
                     GWALWRGAASGAEGAWALALAPLLAQLLACGGLWRPAPAGGWRALLVPASAALRTQMR
                     TVARQQRDFALINTPHALLGLLQDALAVALLVAWSGEAAAGFWGLALRYLKAPATLVG
                     GAVSQALYPRLAAARADDARRLVRRLMALLGALALGLMALLLLAGPWLFERVFGPPWR
                     EAGELARALAPYIAAHFVAAPLAVVTMAWRAQRWAFRLALLGQLALLAALALGLRQGG
                     LLGGAWAVSAVMLPYFGWYFWRLAGWRDIPSPPGVPPREGAP"
     gene            14548..15123
                     /locus_tag="PK242_00565"
     CDS             14548..15123
                     /locus_tag="PK242_00565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOZ92549.1"
                     /translation="MRAALNRLRRRLWRLLFHRMLFGEASAGVLRPATRLSPTVCIEH
                     EPTLTLADDVFIGHFCFIEASGGVRIGGGTQVTNFVSIVSHSTHRALRVAAGLSGAPL
                     EAAVRRAPIVIGERCFIGPHSTIEAGTTLGHGTLVAAHSRVRGVFPDFAVLAGSPAQV
                     VGDTRAADERWLAAHPELLAAYRRWRQGLAQ"
     gene            15120..16178
                     /locus_tag="PK242_00570"
     CDS             15120..16178
                     /locus_tag="PK242_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ92550.1"
                     /translation="MTAPHLGLLGDASSVHVQRWAREMRARGWRVSLVTARPAPIEGV
                     SQIVLRPVRRASDWLWRVGEARRALTQLQPDLVHAHYVTSYGYLAARAGLQPLVLTAW
                     GSDLLVTPRQSALKRWLTAWSLRRARLVTGDSRDLLAAARALAPGVPTQLVHWGVERS
                     RFAPVPWADKPAAEAVSPRNWEPNYRIDVVLRALAQVRAVRPEARLHLLGGGPLEADL
                     RALTGQLGLQDAVRLHGRLDDAGMAAVLARCKLSISVPESDATSVSVLESMACGLAVI
                     ASDLPANRDWLAPEALVAAGDVAALAARWLALLQDDGRAAALGAANAERIARDGDRRV
                     QMDAIDAACRRLLQEAGR"
     gene            16175..17239
                     /locus_tag="PK242_00575"
     CDS             16175..17239
                     /locus_tag="PK242_00575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ92551.1"
                     /translation="MTQDLATPPLPQLLTPGEAGLVSVVAPCRNERAHIDAFCDSALA
                     QRLPEGWRMELLVADGASDDGTRARLAERAAADARLRLLDNPRRIVSSGLNACLAQAR
                     GQVIVRMDIHTRFAPDYVAECIAALARSGADNVGGPWVARGQGGWGRAIAAAFQSRWV
                     VGGARSRDAAFEGEVDTVYLGCWPRASLARVGGFDETLVRNQDDEHNLRLRLAGGRIW
                     QSRRIHSSYRPRDSLRQLFEQQMQYGYWRPFVLRKHRQAGSVRQLVPMVFVAAGAFCA
                     LLAPVFDLGLKAWAAAYAAYLLAASAQAAHQAGEARLAWRLPWVIAAYHLGYGLGSWR
                     GVWGLWRRRAAPQAATRLTR"
     gene            17280..18440
                     /locus_tag="PK242_00580"
     CDS             17280..18440
                     /locus_tag="PK242_00580"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOZ92552.1"
                     /translation="MSVSESFLPFARPDIGEAEIDAVARALRSGWVTTGPETRAFEQE
                     FAAFLGGGVQAIAVNSATAGLHLALEAIGIGPGDEVIAPTLTFTATVEVARYLGADAV
                     LVDVDPVTLNIDPAAIEAAITPRTRAILPVHYGGLACDMAAIHAIARRYGLQVVEDAA
                     HALPASCGGTPVGQLPSAATVFSFYANKTMTTGEGGMVVTRDEALAARMRVMRLHGIS
                     RDAFDRFTSRTPAWYYEVVAPGFKYNLTDLAAALGRVQLARLPAFVQRRQQLAARYLR
                     ELAGLPLVLPPQAPAGDTHAWHLFVLRLADTARVTRDEVVQALADAGIGTSVHFVPLH
                     RQPYWRDRYALRPQDFPVAEAAYQRMLSVPLFTAMSDAEQGRVIAALRACLQ"
     gene            18437..19144
                     /locus_tag="PK242_00585"
     CDS             18437..19144
                     /locus_tag="PK242_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92553.1"
                     /translation="MTPARLAASPWPWFGWSARAWARPWRAFVRDFAGWQGECGLELG
                     AGPRSALAPLMLSLVRQVECSAQDAASLPVIEARHRDLLAPQDAARIAHGVRDLRALH
                     GQWDLIVMKSVLGGVHRLGRSSLADVHTTLEQLVARHLRPGGLLVTLDNGRTALEPLW
                     SRFGARRNGWRFFTRDEFPPATAAYGFGLLGSFSAATRLGRPGRWSDDALYGADLLLS
                     PWVRRHAVLLHVYRKPA"
     gene            19141..19770
                     /locus_tag="PK242_00590"
     CDS             19141..19770
                     /locus_tag="PK242_00590"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOZ92554.1"
                     /translation="MSTTSTDAAKRVFDLALALPALLLALPLMAAIALWVRLDSPGPA
                     WFRQARVGRGGRLFRIHKFRTMQVQAVAGGPLITVAGDARVTRAGRWLRRTKLDELPQ
                     LLDVVRGDMSLVGPRPEVPRYMALYPDEARRLILSVRPGLTDRASIEFRDEERLLAAA
                     SDPERAYVEQVMPIKQRYYLDYVAQRSLTGDVGILWHTLRAVLRRPDSR"
     gene            complement(19835..20479)
                     /locus_tag="PK242_00595"
     CDS             complement(19835..20479)
                     /locus_tag="PK242_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOZ92555.1"
                     /translation="MADAPLTLTSLPLFPLDTVLFPGGWLPLRVFEVRYLDMIGRCQR
                     AGAPFGVVALAQGQEVRTPGAPQERLQPVGTLAHVRTLTQPQPGLMLIECQGEQRFRI
                     DQPHQLRHGLWVADVSLLPPDPVVTIPDDLQAAARTLRRAHDTLRQQPQGLRLPGGDA
                     LDYDDCGWVANRWCELLPLPTATRQHLMSLDSPLLRLELVVDLLSRTGIGGAPR"
     gene            20603..21172
                     /locus_tag="PK242_00600"
     CDS             20603..21172
                     /locus_tag="PK242_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOZ92556.1"
                     /translation="MLYTIFSFLLEIGVTLVGGACLLRLYMRWRRMSFDNPVGQFVLA
                     LSDWLVQPLQRVVPPAGGLDVASLLAAWLLKLVQYALLMALLELPRWGLLPVLALLGT
                     ARLAVSVSTALVIIAAVLSWTGQRSRAHDVFERLCAPLLAPIRRRIRLVGGVDLSPLL
                     LIVLLQVLGIVLSSLQSGLLGPGVPALAG"
     gene            complement(21342..22214)
                     /gene="accD"
                     /locus_tag="PK242_00605"
     CDS             complement(21342..22214)
                     /gene="accD"
                     /locus_tag="PK242_00605"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOZ92557.1"
                     /translation="MSWLEKLLPPKIQKSDAAERRQMPEGLWIKCPDCDTVLYKTDLE
                     QNQNVCPHCGHHHRIGARERLNAFLDGEGRYEIGQEVVPVDALKFKDSRKYPERLKEA
                     LENTGETDALVVMGGAVRSISVVAAAFEFDFMGGSMGSVVGERFVRGVQTAIEQKVPF
                     ICFTATGGARMQEGLFSLMQMAKTNAALTRLAKKGLPYISVLTDPTMGGVSAGFAFVG
                     DVVIAEPKALIGFAGPRVIENTVRVTLPEGFQRAEFLQQKGAVDFICDRRELRQKVAS
                     ILAMLQRQPADAVE"
     gene            complement(22242..23051)
                     /gene="trpA"
                     /locus_tag="PK242_00610"
     CDS             complement(22242..23051)
                     /gene="trpA"
                     /locus_tag="PK242_00610"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOZ92558.1"
                     /translation="MSRIAATFEALQARGRKALIPYVTAGFPYADITPAVMHAMVAAG
                     ADVIELGVPFSDPSADGAVIQKAGERALALGIGLGQVLAMVRSFRAKDGDTPVVLMGY
                     ANPVERYDQKHGDGAFIRDAAAAGVDGVLIVDYPPEECRDFAARLRAAGLDLIFLLAP
                     TSTEARMRQVAEVASGYVYYVSLKGVTGSGALDTAEVGAMLPRIRQHVHIPVGVGFGI
                     RDAETARAIGQVADAVVIGSRLIQQIENQPHERVAAAAADFLRPMRAALDA"
     gene            complement(23048..24313)
                     /gene="trpB"
                     /locus_tag="PK242_00615"
     CDS             complement(23048..24313)
                     /gene="trpB"
                     /locus_tag="PK242_00615"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOZ92559.1"
                     /translation="MSDYHQPDASGHFGRYGGSFISETLTYAINELRDAYARYQHDPE
                     FQREFRHELAHYVGRPSPIYHAARTSRELGGAQIYLKREDLNHTGAHKINNVIGQAML
                     ARRMGKPRIIAETGAGQHGVATATICARYGLQCVVYMGSEDVKRQSPNVYRMKLLGAT
                     VVPVESGSRTLKDALNEAMRDWVANVDHTFYIIGTVAGPHPYPMMVRDFQSVIGQECL
                     TQMPELLAEQHMAARQPDAVLACVGGGSNAMGIFHPYIPFENTRLIGVEAAGQGLDSG
                     KHSASLQRGSPGVLHGNRTYILQDDNGQITETHSISAGLDYPGVGPEHAWLKDTGRAE
                     YVGATDAEALAAFHHLCRTEGIIPALESSHAFAHAMKLAPTMRPDQSILVCLSGRGDK
                     DIGTVADLDGADFYDRPSMRGHVVKGARA"
     gene            complement(24310..25041)
                     /locus_tag="PK242_00620"
     CDS             complement(24310..25041)
                     /locus_tag="PK242_00620"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOZ92560.1"
                     /translation="MTPVSLALAGARTRIKICGLTRAQDVDAAVAAGVDAIGFVRYPR
                     SPRHVDIRRAAELARRLPPMVTPVLLYVNADASEIIADCALLTGATLQFHGDESAADC
                     ARVAARCGRPYWRAARIPAGAAGAFDLIEFAHAFATAQALLLDAHVEGFGGGGKTFDW
                     SLLPPNVAAHLVLSGGLTPANVGDGLRLLRGRGRSLAVDVSSGVEAVGPDGQPLKGIK
                     DAARIFQFVAAVRAADAALAGSPPT"
     gene            complement(25038..25823)
                     /gene="truA"
                     /locus_tag="PK242_00625"
     CDS             complement(25038..25823)
                     /gene="truA"
                     /locus_tag="PK242_00625"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOZ92561.1"
                     /translation="MRIALGLSYLGTPYQGWQSQPTGQTVQDRLEAALTAFATSPIAT
                     LCAGRTDAGVHGLMQVVHFDTELERDAFSWVRGTNRFLPRDIAVQWAQPVPQEFHARA
                     SALARRYAYVVLEAPVRPSLESGRVGWAFRPLDGVAMRRAADRLIGEHDFSSFRASAC
                     QALTPVKQLQRIDIRRCGAYWRFDFEANAFLHHMVRNLMGCLVAIGQGLHPPEWIDSV
                     LAARSRDAAAPTFAPDGLYFLGPRYDARWGLPERTPAYDWLPS"
     gene            complement(25936..28653)
                     /locus_tag="PK242_00630"
     CDS             complement(25936..28653)
                     /locus_tag="PK242_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HOZ92562.1"
                     /translation="MQNKTAMTLLGSIASSHSVRQPWRKGVLASAVAIATCGVSLDAQ
                     ALALGAITVRSALGEPLRAEIEVPQISSEEAATLKANIGSPEAFRAAGVDFPAALAGA
                     RVSLQRRASGQAYLRVTGERTVNEPFLSIVVEANWANGRVVRDYTLLVDPPGRGAPPP
                     VSVTASQATAPPTSAVVHAPRAAPLPPVAAADAGSRPRRDIHPAEAPRQAVSSTGEGA
                     NVTVQRGDTAGSIARAHAIEGVSLDQMLVAMLRTNPDAFINGNVNLLRAGTVLQMPSV
                     EQAGAITRQAARRAIVAQTRDFNAYRLGLAQRSGATRVATAATGSASGGVQPEVRDSR
                     AAAPSPDRLKLSREGAQTGAESQLAQSRQAQEQSDRVAELNKNLSELARLQAASGSAT
                     STGVKLPIGTPPAAAPVAATPAAPAASVAAEPAAPTVATPASASEPASVASVPAPAPA
                     MADAASAVPAAPASAAMAEPAAVPASGVSETVTAPVAAPAPQAAPVPPTTASEEPSFL
                     DALTDNPLLPIAGAGLIALLAGFGVYRWRQSKKGDATMDSSFIESRLQPDSFFGASGG
                     QRVDTREGRPSVGSSSSMVYSPSQLDAAGDVDPVAEADVYLAYGRDMQAEEILKEALR
                     THPGRVSVHRKLAEIYARRRDARALEMVASEAWNHVKGEGADWAAIASLGSELDPDNP
                     LYKPGGVPMMRSAATATTPQPRFGADTEPQTAQLQDKRDSKPDSSSIPLDLELDLDDA
                     PVRASAVTSVPPSVGNTSAMPSHHGPEPAIAAAPEVKPVPIALPDFDLDLDTPTTVAP
                     ALAAAVATQADDEPPSVAQPYVASKAGMIDFDMEALSIDPDSRSGVDPKTEQPDDADD
                     DPVATKLALAQEFHAIGDTEGARTLVREVIAEATGSLRMRAERFLAELD"
     gene            complement(28854..29990)
                     /gene="asd"
                     /locus_tag="PK242_00635"
     CDS             complement(28854..29990)
                     /gene="asd"
                     /locus_tag="PK242_00635"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOZ92563.1"
                     /translation="MKTGNVVGLVGWRGMVGSVLMDRMQAERDFDLIEPIFFSTSNAG
                     GAAPAQARNERQLKDAHDIEALKKCDIILTCQGGDYTHAVYPKLRAAGWGGHWIDAAS
                     ALRMEKNAVIVLDPVNLPVIQNALAKGGRDWIGGNCTVSCMLMGVGALYKAGLVEWMS
                     TQTYQAASGGGAQHMRELLAQFGTLNHEVRDLLSDPTSAILEIDRKVIGKQRALDDAE
                     KANFGVPLGGSLIPWIDKDLGNGMSREEWKGMAETNKILGQGEGFDTPAVPVDGYCVR
                     IGAMRCHSQALTFKLRKDVPVADIEAMIAADNDWVKVVPNNRDATLRDLTPVAVTGTL
                     TIPVGRIRKMAMGPQYLGAFTIGDQLLWGAAEPLRRMLRILLQD"
     gene            complement(30457..31527)
                     /gene="leuB"
                     /locus_tag="PK242_00640"
     CDS             complement(30457..31527)
                     /gene="leuB"
                     /locus_tag="PK242_00640"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOZ92564.1"
                     /translation="MKIAVLPGDGIGPEITSEAVKVLQALGLKLELESAPVGGAAYEA
                     AGHPLPESTLNLARAADAILFGAVGDWKYDTLDRPLRPEQAILGLRKHLGLFANFRPA
                     ICYEQLTAASSLKPELVAGLDILIIRELTGDIYFGQPRGRRQAPDGHFPGAEEAFDTM
                     RYTRPEVERIARVAFEAARQRSRRVTSVDKANVLETFQLWKDVVTEVHKDYPDVELSH
                     MYVDNAAMQMVKAPKAFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLNDKKQGLYE
                     PSHGSAPDIAGKGVANPLATILSAAMMLRFSLGQPEAADRIEAAVRKVLAQGLRTPDI
                     WSEGTTRVGTREMGEAVVRALD"
     gene            complement(31629..32285)
                     /gene="leuD"
                     /locus_tag="PK242_00645"
     CDS             complement(31629..32285)
                     /gene="leuD"
                     /locus_tag="PK242_00645"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOZ92565.1"
                     /translation="MDAFTTHKGLVAPLDRENVDTDAIIPKQFLKSIRKTGFGPNLFD
                     EWRYLDHGEPGMDPASRKPNPDFVLNQPRYQGASILLARQNFGCGSSREHAPWALQQY
                     GFRAILAPSFADIFFNNCFKNGLLPIVLPESAVDRLFHDVAAFPGYQLTIDLQRQVVV
                     KGDGTELAFDIDPFRKHCLLGGLDDIGLTLQHKDAIARFEAERLASKPWLAHTMGRPA
                     "
     gene            complement(32476..32598)
                     /locus_tag="PK242_00650"
     CDS             complement(32476..32598)
                     /locus_tag="PK242_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HOZ92566.1"
                     /translation="MKKVLALVAAALMLAGCNTVQGVGKDVQKAGSAVENAGKK"
     gene            complement(32618..34072)
                     /gene="leuC"
                     /locus_tag="PK242_00655"
     CDS             complement(32618..34072)
                     /gene="leuC"
                     /locus_tag="PK242_00655"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOZ92567.1"
                     /translation="MTPRTLYDKIWDEHVVRTEEDGTALLYIDRHLVHEVTSPQAFEG
                     LRQAGRKVWRTSSVVATADHNTPTTGWERGYDGIEDPISKEQIVTLDANMQAHGAAAY
                     FPFMNQRQGIVHVIGPENGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLAT
                     QTLIAKKARNMLVRVNGAVAPGITAKDIVLAIIGRIGTAGGTGHTIEFAGQAIRDLSM
                     EGRMTVCNMAIEAGARAGLIAVDDKTIDYVKGRPMAPGTDPVTGRFVGGPEWDQAVAS
                     WRSLHSDDGAHFDTVVELDAQAIVPQVTWGTNPEMVVGVDARVPDPDKEKDPVQRGAM
                     ERALAYMGLIPGKPIADIHVDKVFIGSCTNSRIEDLRAAAVVVKRLGRRLAPNVKLAM
                     VVPGSGLVKQQAEAEGLDKIFTAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNF
                     EGRQGQGGRTHLVSPAMAAAAAVHGHFVDIRQIS"
     gene            complement(34353..35648)
                     /locus_tag="PK242_00660"
     CDS             complement(34353..35648)
                     /locus_tag="PK242_00660"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOZ92568.1"
                     /translation="MKLSDNKAKLSFSNGSPDIELPVYQGSIGPDVIDIRKLYGQTGM
                     FTYDPGFLSTASCQSAITYIDGDKGELLYRGYPIEQLAQKCDYLDTCYLLLNGELPNP
                     TQRSDFHKLVLKHTMVNEQMQFFLRGFRRDAHPMAVLTGLIGALSAFYHDSTDIHNPE
                     HRHISAIRLISKLPTLVAMAYKYGVGQPYMYPRNELSYAGNFLRMMFGTPCEDYKVNP
                     VLEKAMDRIFILHADHEQNASTSTVRLCGSSGTNPFAAIAAGVACLWGPAHGGANEAC
                     LNMLEDIQRNGGIAKVGEFMEQVKDKNSGVKLMGFGHRVYKNYDPRAKLMQETCNEVL
                     AELGLEKDPLFALAKQVEKIALEDDYFVQRKLYPNVDFYSGIVQRAIGIPVSLFTGIF
                     ALARTVGWIAQLNEMLADPEYKIGRPRQLFTGSPRRDVP"
     gene            complement(35699..35971)
                     /locus_tag="PK242_00665"
     CDS             complement(35699..35971)
                     /locus_tag="PK242_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HOZ92569.1"
                     /translation="MESERIDEKALGMLRWRCRRGMLENDLLIERFFQRHAEQLTASQ
                     AAALTQLMDLSDNELLDLLLRRKEPDGDLNTPDIRQLLTLLRPAAA"
     gene            complement(35998..36702)
                     /locus_tag="PK242_00670"
     CDS             complement(35998..36702)
                     /locus_tag="PK242_00670"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOZ92570.1"
                     /translation="MQRTFKIYRYDPDKDAKPYMQTVQVELDGHERMLLDALLKLKAQ
                     DPSLSFRRSCREGVCGSDAMNINGKNGLACLTNMKTLSGDIVLKPLPGLPVIRDLIVD
                     MTQFFNQYHSIKPYLQNDQPTSPSKERLQSPEEREELNGLYECILCASCSTSCPSFWW
                     NPDKFVGPAGLLQAYRFIADSRDQATGERLDNLDDPYRLFRCHTIMNCVDVCPKGLKP
                     AAAISKIKELLVRRGI"
     gene            complement(36735..38540)
                     /gene="sdhA"
                     /locus_tag="PK242_00675"
     CDS             complement(36735..38540)
                     /gene="sdhA"
                     /locus_tag="PK242_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HOZ92571.1"
                     /translation="MSYTKDQVITRKFDVVIVGAGGSGMRASLELARAGLNVACLSKV
                     FPTRSHTVAAQGGVSASLGNMSEDNWHYHFYDTIKGGDWLSDQDAVEFMCREAPNVVI
                     ELEHFGMPFDRNPDGTIYQRPFGGHTANYGEKPVQRACAAADRTGHAMLHTLYQKNVE
                     SKTNFFVEWMALDLIRNSAGDVVGVTALELETGDLYELHAKAVLLATGGAGRIFAAST
                     NAFINTGDGLGMAARAGIPLQDLEFWQFHPTGVAGAGVLLTEGCRGEGAILLNSNGER
                     FMERYAPTLKDLAPRDFVSRSMDQEIKEGRGCGPNKDYILMKLDHLGADTIRKRLPSV
                     EEIGHNFANVDITKEPIPVVPTIHYQMGGIPTNIHGQVVTWDGEKNNIVNGLYAVGEC
                     AAVSVHGANRLGTNSLLDLLVFGKSAGKHIVQFVRSYGEQAAVPADASERTLARLNQL
                     ENSTDGIYAQDLANEIRQTMQQHAGVFRTQKGMDEGVVKIAGIRERVGQVTLNDKSRV
                     WNTARMEALEVDNLIEVAQATMVSAAARHECRGAHTVYDYEHPADHPTAPLGRDDANW
                     LKHTLWDSATNSLSYKPVNMKPLTVDSIPPKVRTF"
     gene            complement(38586..38951)
                     /gene="sdhD"
                     /locus_tag="PK242_00680"
     CDS             complement(38586..38951)
                     /gene="sdhD"
                     /locus_tag="PK242_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HOZ92572.1"
                     /translation="MSTTYGNKRLVVGAHYGWRDFLVQRVSGALIAAFTLIVLLQVLF
                     TQGPIGYDTWAGIFAAQWMKALTFSVIVALIWHAWIGMNSIWLDYVKAAGVRLLLQTF
                     TVIWLVSCGGWALQALWRL"
     gene            complement(38975..39406)
                     /gene="sdhC"
                     /locus_tag="PK242_00685"
     CDS             complement(38975..39406)
                     /gene="sdhC"
                     /locus_tag="PK242_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HOZ92573.1"
                     /translation="MSEATKPRPEFRNINIFNDVRTYRLPLAGWVSILHRASGALMFL
                     LLPFIIWLFDKSVSSEISFGQFSAAFSSGLGAFPAWFVKLVVLALIWAYLHHLLAGVR
                     HLYLDISHRTTKEFGRQSAVATLVVSIVLTLALGAKLFGLY"
     gene            complement(39564..40370)
                     /locus_tag="PK242_00690"
     CDS             complement(39564..40370)
                     /locus_tag="PK242_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOZ92574.1"
                     /translation="MAHAAPSSTPTESAPPALAPATAWGGQAPAFSPLYQQIKSLILQ
                     SLQQGEWKPGEVIPSEFDLAARYKVSQGTVRKAVDELAADNLLVRRQGRGTFVATHAE
                     QQVQYRFLRLQPDRGTADSEGPAQRDFVDCKRQRASADVARLLGLRAGDPVLQARRLL
                     RFAGVPTILEDLWLPAGPFKGLTAERLSQYHGPMYALFEAEFGVRMVRADEKLRAVLP
                     DPTQAALLEVTPATPLLSVERIAYTYNDEPMELRRGLYRTDTHHYRNALS"
     gene            40559..41545
                     /locus_tag="PK242_00695"
     CDS             40559..41545
                     /locus_tag="PK242_00695"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOZ92575.1"
                     /translation="MSKKPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEI
                     PDEKAQKALKGVMMELEDCAFPLLAGMEAHSDPMTAFKDTDYALLVGARPRGPGMERA
                     DLLAANAQIFTAQGKALNAVASRNVKVLVVGNPANTNAYIAMKSAPELPARNFTAMLR
                     LDHNRAASQIAAKTGKAVKDIKKLAVWGNHSPTMYADYRFATIDGASVKDMINDQEWN
                     ANVFLPTVGKRGAAIIDARGLSSAASAANAAIDHMRDWALGTGGEWVTMGIPSQGWYG
                     IPKEVMFGFPVTCENGEYKVVEGLPIDAFSQQCIDKTLAELEGEKDGVKHLL"
     gene            41696..42682
                     /locus_tag="PK242_00700"
     CDS             41696..42682
                     /locus_tag="PK242_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOZ92576.1"
                     /translation="MTAVARHPRQVLLGAQAGAVVLPVCDHYSGVEARMRKSLQLQAE
                     MTQEFGTCVFDVTLDCEDGAPVGGEAEHAQCVTELALAAAPDARVGVRVHPVDHPAFE
                     ADMAQIAGKAGHRLAYIMIPKVESVEDVVHAERALRASFAPGLPLQVLIESPAAVHRA
                     FDIAAHPGVQSLSFGLMDFVSAHAGAIPAEGMGIEGQFTHPLVLRAKLEIASAAHAFG
                     KVPSHCVVTEFSDVQAMRMAARRAAREFGYARMWSIHPSQIRPILEAFAPDDRQIDLA
                     SAILLAAQQADWAPISHQGALHDRASYRHFWQVLERAHATGRHLPGDVAHWF"
     gene            42739..43326
                     /locus_tag="PK242_00705"
     CDS             42739..43326
                     /locus_tag="PK242_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92577.1"
                     /translation="MKPFAMTSLAIVLGLSMGAVQVASAADKPAPAKKVTTQAKKAPT
                     KKVPVKQAATSKGVPAKTAKAVEANTPVQTLSQRLTDTELEIAKTIQTGRIQCELGAD
                     VTVSADEKNPGFFHVVAGKQRYYMHPVESRTGAVRMEDNRAGAMWLQLGNKSMLMNQK
                     LGQRVADECVTPAQREFALQMKSSNQPGLLDGQTK"
     gene            43439..46030
                     /gene="acnB"
                     /locus_tag="PK242_00710"
     CDS             43439..46030
                     /gene="acnB"
                     /locus_tag="PK242_00710"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HOZ92578.1"
                     /translation="MLQAYRAHAAERAKLGIPPLALDAKQVAELIELIKNPPAGEEQF
                     LMDLLTHRVPPGVDDAAKVKASFLAAVAHGDIQVGLISKAKATELLGTMVGGYNVHPL
                     IELLDDAEVAQVAADALKKTLLMFDYFNDVATKAKAGNAKAKEVMQSWADAEWFTSRP
                     KVAEKITVTVFKVPGETNTDDLSPAPDATTRPDIPMHYLAMLKNTRPDAAFKPEQDGV
                     RGPIQFIEDLKKKGHIVAYVGDVVGTGSSRKSATNSVIWATGEDIPFVPNKRFGGVTL
                     GGKIAPIFFNTQEDSGSLPIEVDVSKMEMGDVLDIFPYAGKIEKAGEKIADFKLKSDV
                     LLDEVQAGGRINLIIGRALTGKAREFLGLPASTAFRLPQAPAESKAGFTLAQKMVGRA
                     CGLPEGQGIRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAA
                     YPKPVDVKTHRTLPEFISSRGGVALRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFGAATGVMPLDMPESVLVRFKGQLQPGVTLRDLVHAIPLYAIKAGLL
                     TVAKAGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTIKLNPEPIKEYLR
                     SNIVLMKNMIADGYQDAKTLARRIEKVEAWLANPQLLEADKDAEYAAVIEIDMNEITE
                     PIVCCPNDPDDAKFLSEVAGTKIDEAFIGSCMTNIGHFRAAAKLLGGQRDIPVKLWVA
                     PPTKMDQNELIKEGHYAAFGTAGARTEMPGCSLCMGNQAQVHEGATVISTSTRNFPNR
                     LGKNTNVFLGSAELSAIASRMGKLPTKEEYLKEMGVIDQDKASVYRYMNFDQIEEYAE
                     VAKGVAA"
     gene            complement(46380..46925)
                     /locus_tag="PK242_00715"
     CDS             complement(46380..46925)
                     /locus_tag="PK242_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HOZ92579.1"
                     /translation="MIRLNSFTTAAAVIAAGAALTACGSMNKGTATARASLAPTAAIT
                     PNPTSGSATFTALAHGVRVAGEVRGLVPGSEHGFHIHEKGDCGNNGNASGGHFNPTGG
                     MHGKFGGAGTHAGELPSLVADAAGVARFSVDVHTISLTDGAANNVVGRALVVHRDRDD
                     FSTQPAGNSGPRIACAVITRE"
     gene            complement(47164..48837)
                     /locus_tag="PK242_00720"
     CDS             complement(47164..48837)
                     /locus_tag="PK242_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOZ92580.1"
                     /translation="MPLPPPPAQRGARLSPDVIDLATRRAKTSAATPPSQPSSPARSA
                     PSARTAPPSGSPAPAALLQAPAPAPVSSTPKAPPARRPKSTPGARKARTQGPARLFVL
                     DTNVLLHDPMALFRFKEHDIFLPMIVLEELDGHKKGMSEVARNGRQTSRLLDALAGVR
                     GADMAQGLKLDATGHVEASGHLFFQTTPLDATMPAALPQGKADNQILGVVAALETQHA
                     PREVVLVSKDINMRVKARALGLHAEDYENDKTLDDGDLLYAGVLQLPTDFWTRQSKTV
                     ESWQSGSATFYRISGPVVPSLQINQFVFFEAPGEPSLYARVTEIQGKTAVLRTLKDYT
                     HLKNAVWGVTARNREQNFALNLLMDPEIDFVTLAGTAGTGKTLLALASGLTQVLDDRR
                     YTEIIMTRATVSVGEDIGFLPGTEEEKMGPWMGALDDNLEFLSKGDGSGAGEWGRAAT
                     NELIRSRVKIKSMNFMRGRTFMNKWIIIDEAQNLTPKQMKTLITRAGPGTKIICMGNL
                     AQIDTPYLTEGSSGLTYAVDRFKGWPHGGHVTLARGERSRLADFASEVL"
     gene            complement(49018..49494)
                     /locus_tag="PK242_00725"
     CDS             complement(49018..49494)
                     /locus_tag="PK242_00725"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOZ92581.1"
                     /translation="MAIVVNKPLPEFEAMATGGVKVSNSSHLGQAVVLYFYPKDNTPG
                     CTTEAMQFRDKYKDFVKAGAIVFGVSRDNMASHDSFKTKLELPFELIADTEEKMCHMF
                     GVVKNKIMYGKKVKGIERSTFLINAEGIVVQEWRGLKVPGHVEDVLKAVKVLKKAA"
     gene            complement(49611..50015)
                     /locus_tag="PK242_00730"
     CDS             complement(49611..50015)
                     /locus_tag="PK242_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HOZ92582.1"
                     /translation="MKFQPDQFGSAITSYGPGWIAIGGQRLEHSLVLRSDGEHALWNC
                     SRFDELTEAHFTLLVALQPQLVLFGSGARLRFPRPAWVRGLIEAGIGVETMDTGAACR
                     TYNILAGEGRRVVAALLVEAGTSGTSGTDRLE"
     gene            50095..>50726
                     /locus_tag="PK242_00735"
     CDS             50095..>50726
                     /locus_tag="PK242_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ92583.1"
                     /translation="MKTIHKSTKLANVLYDVRGPIVDAARQMEDEGQKIIKLNIGNLA
                     PFGFEPPEEIVRDMIRNLPDSAGYSDSKGIFSARKAVMHYTQEQGIEGVTIDDIYLGN
                     GASDLIGMATNALLSEGDEMLVPAPDYPLWTAVASLSGGKPVHYRCDEDNGWMPNLAD
                     IRARITPATRAIVVINPNNPTGALYSDELLRGIVQIAREHGLVIMADEVY"
BASE COUNT         7469 a        16655 c        18156 g         8446 t
ORIGIN      
        1 tgcaccgccg cgccgagatc gcgccctggc tcaagggcat gcaggagcgc cacccattcg
       61 ccgaattcgt gcccatgtcg gccaagaacc cgcgcgacgt ggcgcgcctg ctggagatct
      121 gcgcccgtta cctgcccgag cagccctggt ggtacggcga agacgaactg accgaccgca
      181 gcgaaaaatt cctggccagc gaaacggtgc gcgaaaagct gtttcgcctc accggcgacg
      241 agctgcccta cacctccacc gtggtgatcg agcagttcaa ggaagaaaaa agccgtgccc
      301 acggccgcct gctgcgcatc gccgccacca tcgtggtcga gcgcgaaggc cacaaggcga
      361 tggtcatcgg ccagcagggc gagcgcctca agcgcatcgg caccgaagcg cgccaggagc
      421 tcgaaaagct gctggacgcc aaggtgttcc tggaaatctg ggtcaaggtg cgctccggct
      481 gggccgacga cgaggcgcgc gtgcgcagct tcggctatga atgacagatg acgaatgacc
      541 tcgcctgcgg cttcgatggc gaatgcgcga atggccggcc gtcgtctgtc cgaagtcaat
      601 cgtcattggc atgaagcgcg tcgttcacga gcccggctac gtgctgcacc actacgactg
      661 gagcgaatcc agcctgatcg tcgaggcctt cacgcgcgcg cacggccgcg tggccctggt
      721 ggccaagggc gccaagcggc cgtcgtccaa cttccggccg gtgctgctgc cgctgcagcc
      781 gctgctgctg tcctggggtg gtgacggcga gatccgcacg ctcaaggcgg ccgagtgggg
      841 cggcgggcag gtgatgccgc gcggcgatgc gctgctgtcg ggcttttacg ccaacgagct
      901 gctgctgcgc ctgctggcgc gcgaggatgc ccacccgcgc ctgttcgacg cctatgccgc
      961 cctggtgcat gcgctggcgg cgggcgatgc cgagccgctg gcggccgccg cgctgcgcgc
     1021 cttcgagctg ctgctgctgc gcgaaaccgg gcacctgcca gcgctcgacg cccagaccct
     1081 gaccctggcg ccgctgcagg cgagcgagcc ctaccggctg gtggccgaag gcggcctgcg
     1141 cgccgcccac gccgacgacg cggcctggct gccgggccag agctggcgac gactggccgc
     1201 ctccctggcg gacgaagccc cctggaccgc caccctgacg gtggtggtcg gcctgccggg
     1261 tgaacagcgc ggcagcctgc gccgccagct tggcgagctg ctccattacc atggcggcgg
     1321 tgccacgttg cgcacgcgcc agcttctgca ggacgcccag cgcctgatgg cgacctgaga
     1381 cgcgcagcca ccgagcccgc catgcccacc cagctttccg tcaacgtcaa caaggtcgcc
     1441 ctggtgcgca acacgcgcca cctgggcatt cccagcgtca cccgcgccgc ccagctgtgc
     1501 ctgcaggccg gcgcgcacgg catcaccgtg cacccgcgcc cggacgcgcg ccacattcgc
     1561 gccgacgacg tgcatgcgct ggcggcgctg atgcgcggct ggccggggcg tgaattcaac
     1621 atcgagggca acccgacgca gaacctgatg gacttcatcc gcgccgtgcg cccggcgcag
     1681 gccaccttcg tgcccgacag cgaagaccag ttcaccagcg accacggctg ggacctggcc
     1741 gacgccggcg tggcggcccg cctgcggccg ctgatcgacg aatgccaggg cctgggcgtg
     1801 cgcgtgagcc tgttcatgga cccgctgccc gaactgatgg cggccgcgcg cgcggtcggt
     1861 gccgagcgcg tggagctcta caccgagccc tacgccgccg cctggggcac gcccgaacaa
     1921 gggcgccagc tgcagcgcta cgccgtcgcg gcgcaggcgg caggcgacgc cggcctgggc
     1981 gtcaacgccg ggcacgatct gaacctgcac aacctgggca ccttcgtggc gcgcgtgccg
     2041 gggctgctgg aagtgtcgat cggccacgcc ttcatcgccg acgcgctgga gcgcggctac
     2101 gccgccacca ccgaggccta cctgagctgc atcgcccagg ccgggcgctc ctgaatgaat
     2161 agcttgcggc gcttgctgca tgcgcgttgc aggcgaattc aagcttgatc ccatgatcca
     2221 cggcatcggc accgacatct gcgacgtgcg ccgcatccgc gccagcgtcg agcggcacgg
     2281 cgagcgcttt gcgcagaaga tcctgagcga ggccgaactg gccgtgtggc gccgccgcag
     2341 cgcccgctgg cccgagcgcg ggctgcgtta cctggccaca cgcttttcgg ccaaggaagc
     2401 gttttccaag gcggtcggcc tgggcatgcg catgcccatg cactggcgcc gctgcgaaat
     2461 cgccaaccag cccagcggcc agccgtgcat cgtgttgcac ggaccgctga aggactggtt
     2521 cgaggcccgg cacctggtgg cgcacatcag cgtgagcgac gagagcgact acgcggccag
     2581 cttctgcgtg gtcgagcgcc tgccgccggt tcgtgaatga aaagcggctc tggcgcgctc
     2641 ccggcaagcg ccagaagcta tgataattgt agtcaccgag cttaccgaaa ggccccatgc
     2701 agcacgcccc cctgatcatc gacatcgccg gcaccgccct gaccgaggac gatggtcgcc
     2761 gcctggcgca ccccctggtg ggcggcctga tcctgtttgc ccgcaactgg caggatcgtg
     2821 aacagctcgg cagcctgtgc gccgaagtca ggcgcctgcg gcccgaactg ctgatctgcg
     2881 tcgatcacga aggcgggcgc gtgcagcgct ttcgcagcgg cggcttcacc cacctgccgc
     2941 ccatgcgcgc gctgggcgag ctgtggatgc aggacgccat gcgcgcgcag gacgcggcca
     3001 gcgcctacgg ctacgtgctg ggcgccgaac tgcgcgcctg cggcgtcgat ctgtcgttca
     3061 ccccggtgct ggacctggac tggggcccct cgggggtgat cggcgaccgc gctttccatg
     3121 ccgacgcccg cgtcgtcacc atgctggcca agagcctggc gcacggcctg cggcgtgccg
     3181 gcatggcctg ctgcggcaag catttcccgg ggcatggctt cgtcgccgcc gattcgcaca
     3241 ccgcgttgcc gcgcgacacg cgcacgctca aggccatcct ggccgccgac gccgcgccct
     3301 acggctggct gagcagcgtg ctggacgcgg tgatggccgc ccacgtcgtc tatccgcggg
     3361 tggacgcgcg gccggccggc tattcggcgc gctggctgca ggcggtgctg cgcgggcggc
     3421 tggggtttgg cggcgccatc ttcaccgacg acctcagcat gcaagccgcg cgccacctcg
     3481 aaggtcgcac cgtcaccccg gtcgaggcgg tgctggccgc gctcgacgcc ggctgcgacc
     3541 tggcgctgct gtgcaaccag tcgctcggtg gcggcgcggt gctggacgaa gtgatcgacg
     3601 gcctggcgcg cgcccaggtt cagggccgct ggcagccgcg cgaggccagc gccgcgcgct
     3661 gccaggcgct gctgccgcgc gagccggcgc ccgactggga cgcgctgatg cgctcgcccg
     3721 cctacctgca cgcgctggcg ctggttccct gagccgcgcc gggtgggcgc cgcggctggc
     3781 cgcgccgtgc gagcagcgcc gtcaggccga tgcggccccg ggccgcgcca gcgccgcatc
     3841 gcgcagccgg ccggcgggga aatccaccga aaacaccgac ccctgtcccg gcacgctggc
     3901 gatgtgcagg cgggcgtcgt ggcgctgcag cacatgcttg acgatggcca ggcccaggcc
     3961 ggtgccgccg gtgtcgcgcg agcggctgcg gtcgatgcgg tagaagcgct cggtcaggcg
     4021 cggcagatgc tcggcggcga tgccggggcc ggtgtcgtgc acggcaaagc gcgcgccgcc
     4081 gtccggcagg cgctgccatt gcaccgtgat gcgcccgccc gtcggggtgt agcgcaccgc
     4141 gttggtcacc aggttggaca gcgcgctttg cagctcggcc gggtcacccg ccagctcgcc
     4201 cgctgcgcgc agctcgtcct gggtcggaaa caccagcgtc tggggcgcgg cgccagccgg
     4261 tgtgagcagc gccgacaggg tgtgcgcctc gtcctcgcac gggcgcagca gggcggccac
     4321 cggcgtccag gtctgaaagc cgggcagggg actgccttcc aggcgcgaca gcgtcagcag
     4381 gtcctgcacc aggtgctgca tgcgctgggc ctgctgcgcc atcaggccca gatagcgcgc
     4441 ccgctcggcc tctgacagcg gcagcgactg catggtttca atgaagccgg ccagcacggt
     4501 cagcggggtg cggatctcgt gtgacacgtt ggccacgaag tcgcgccgca tggcctcggc
     4561 ctgctccagc tgcgtgatgt cgcgcgacag cagcagcctg cgcccttcgc cgtaggggtg
     4621 caggcgcacg gccaggcgtt gcggccgctc gggcgtgctg gcgcgtgcgt tcagcaccag
     4681 gtcctgtgcg tcgtccgggc gcgccaggta ggcgctgaag ttcggatcgc gcagcaggtt
     4741 gccgatctgc tgctgcagat cgcgcccggc gtcgaggccg aaatgctgcg ccgccgtttg
     4801 gttgaaccat tccatgtgac cctgtggatc cagcagcacc acgccgttgg gcgaagcctg
     4861 cagcgccgcc aggaactcct gcaggcgttg cccgctggcc agcgcctgct gctcctgctg
     4921 gcccagccgc cgccgcatgc gcgctgccgc ctcgccccac agtccgcgca gcacgggggc
     4981 actggacgcc gcaccgtcgc cgcgcagcca gcggatcacg cgctgcccgc tccaggtttc
     5041 ccagccgaag gccagccagg cggcaagcac cacgccgccg accacccccc atgggcccag
     5101 cagcagcccg cccgccgcgg cgcaggccag ctgcagcaga ctgaacatca acacacgcca
     5161 gaccatgggt ttgcagcaat tctgaagaaa tcgggctcca gcgcaggcgc atcaagcgcc
     5221 ggaagctatg gatttgctag cgtctggctg ggccgtcagc cgataacccg cgccgcgcac
     5281 ggtctcgacc atggtggcgg cgtcgcccag ggcctcgcgc aggcgcttga cgtgcacgtc
     5341 cacggtgcgc tcctcgatgt agacatggtc gccccagacc ttgtcgagca gctggccgcg
     5401 gctgtgcacg cgctcggcgt tgcgcatcag gtagtgcagc agcttgaact cggtggggcc
     5461 gagcttgagc ggctggcccg cgtggctcac gcggtgcgtg gccgggtcga gcgagagcgc
     5521 gccgatggcc acgctctcgc cgctgtgctc gggcgcgcgc cggcgcagca cggcgcggat
     5581 gcgcgcgagc atttcctggg tggagaacgg cttggtgatg tagtcgtcgg cgcccgcgtc
     5641 caggccggcg atcttgtctg gctcgtcgcc gcgcgcggtc agcatgatga tgggcacggc
     5701 gcgcgtgcgc gggtggccgc gccatttgcg cgacagctcc aggccgcttt ggcccggcag
     5761 catccagtcg agcaggatca tgtcgggcag cacggcatcg agctcgcgct gggcgctgtc
     5821 gccgtcctcg gcccagatgg gcgcgaagcc gttgtggcgc aggttcacgg aaatcagctc
     5881 ggcaatcgcc ggctcgtcct cgacgatcag gatcgtgggc agctttctca ttggatggtg
     5941 gattcgatgt cggcgacggg gcggtggcgc acgtccttgc cctcgacgag gtagatgatg
     6001 agctcggcga tgttcttgga atggtcgccg atgcgctcta tggccttggc caggaacagc
     6061 agatcgaggc tggcagagat ggtgcgcgcg tcctccatca tgtaggtgat gagcttgcgc
     6121 acgaagccgt cgaactcgcg gtcgatgagg tcgtcctcct tgaggatggc gaccgcggcc
     6181 ttcacgtcca ggcgcgcgaa cgaatcgagc gccttgcgca gcaggcccga ggccaggtcg
     6241 gccgccacgc gcaggtcgga gcttggcagc gagcgcgccg cgccgctttc gatgatggag
     6301 cgcaccatgc gcgccatgcg tgcggcctcg tcgcccatgc gctcgaggtt ggccgtggcc
     6361 ttggagaagg ccatcaacag gcgcaggtcg cgcgccgtgg gctggcgccg cgcgatgatg
     6421 gtgatgagct cgtggtcgat gtccacctcc atctggttga cgcggctttc gatggactcg
     6481 acctgcgcgg ccgcctccag gctgaagtgc gagagcgcgg aaatcgcctc gcggatctgc
     6541 gattcgacca ggcccccgag ctccatcacg cgggccgaga cctggttgag ttcgctgtcg
     6601 aactgggtgg agagatgttt gtctgtcatg gtgttctttc ttgactccct cgcccccttg
     6661 ggggagaggg tgggggtgag ggggcgcagc gcctcttgcc gagaccctca cccccgccct
     6721 ctcccgcacg cgggagaggg agttccgctg tcagccaaag cggcccgtga tgtagtcttc
     6781 cgtttccttg cgctcgggct tgaagaacat ctgctcggtg gcaccgaact ccacgaggtc
     6841 acccaggtac atgtaggccg tgtagtcgct gcagcgcgct gcctgctgca tgttgtgcgt
     6901 gacgatgacg acggtgtagt cgttcttgag ctcggcgatc agctcctcga tcttggccgt
     6961 ggagattggg tccagcgccg agcagggttc gtcgagcagc agcacctcgg gcagcacggc
     7021 aatgccgcgc gcgatgcaca ggcgctgctg ttggccgccc gacaggcccg cgccgctttg
     7081 cgtgagcttg tccttgacct cgttccacag cgcggccttt ctgagcgccc attcgacgcg
     7141 ctcgtccatg tcggatgcgt tcaggttctc gaacagcttc acgccgaagg cgatgttgtc
     7201 gtagatcgac atcgggaacg gcgtgggctt ctggaacacc atgccgacct tggcacggat
     7261 cagcgccacg tcctgcttgg actgcagcag gttctcgccg tccagcacga tctggccctc
     7321 ggcgcgctgc tcggggtaga gctcgaacat gcggttgaag gtgcgcagga gcgtggactt
     7381 gccgcagccc gatggcccga tgaaggccgt gaccttcttc tcggggatgt ccaggttgat
     7441 gcccttgagc gcgtggaact tgccgtaata gaagttcagg tcacgcaccg cgatcttgga
     7501 gggcacggcg gggtgggagg tggggggcat gaatgtcttc cggaaaagaa ggggttactt
     7561 ttgacgggtc agcacgcgcg ccaggatgtt caggcccagc accgcgacgg tgatgaggaa
     7621 cacggccgcc caggcgagct gttgccagtt ctcgtagggg ctcatggcga acttgaagat
     7681 ggtcacgggc aggttggcca gcggcttgga catgtcgccg ctccagaact ggttgttcag
     7741 cgcggtgaac aagagcggcg cagtctcgcc cgcgatgcgc gccacggcca gcagcactcc
     7801 ggtgatcacg ccggcgcggg cggcgcgcag cgtcaccatg gtgatgacct tccacttcgg
     7861 cgtgcccagg gcgtaggcgg cctcgcgcag gctggcgggc acgagcagca gcatgttctc
     7921 cgtcgtgcgg atgaccacgg gcacgacgat gagcgccagg gccacgacgc cggcccagcc
     7981 ggagaagctc ttgaagcgca ccacgaccac cgtgtagacg aacaggccga tgacgatgga
     8041 gggcgccgac agcaggatgt cgttgacgaa gcgcgtggcc ggcgccagcc agccgcgctt
     8101 gccatactcg gccaggtaga tgccggccag aatgccgacg ggcgtgccca cgaaggtggc
     8161 cagcagcacc atcatgaacg agccgtagag cgcgttggcc aggccgccgg cctcgttggg
     8221 cgggggcgtc atctcggtga acagtgacag cgacaggcca cccaggccca ggcgcagcgt
     8281 ctcccacaga atccacacca gccagaacac gccgaaggcc atggcggcga gcgacagcgt
     8341 cagcgccacc tggttcacgc gcttgcgcgc cgcgtagcgc cggccgcgtg cctgctccag
     8401 tgcggcccgg ctcagcaatt tttgtgacgt ggtggcggcg ttcatgcgcg tgctccttcg
     8461 gccttctgca ggcggctcag caacagcttg gaggccgaca gcaccacgaa ggtgatgaag
     8521 aacagcacca ggcccagata gatcagcgac gcctggtgca ggccctcgcc ggcctcggcg
     8581 aactcgttgg ccagggccga ggtgatgctg ttggcggcct cgaacaccga cagcgaggcg
     8641 agctggttca tgttgccaat cacaaaggtc acggccatgg tctcaccgag ggcgcggccc
     8701 aggccgagca tgatgccgcc gagcacgccg gccttggtgt agggcaggac gaccttccag
     8761 atcacctccc aggtcgtggc gcccaggccg taggccgatt ccttgagcag cgccggagtc
     8821 acctcgaaca catcgcgcat gaccgaggcg atgaacggaa tgatcatgat cgcgaggatg
     8881 atgccggccg acagaatgcc tatgcccacg ggcgggccag agaccagcgc gcccagccag
     8941 ggcacgcccg agagcaggct ctgcagcggc tgctgcaccc aggtggccag cagcgggccg
     9001 aacaccatca gcccccacat gccatagacg atggagggca cggcggccag cagctcgatg
     9061 gccgtgccca gcgggcgctt gagccaggcc ggggacagct cggtgaggaa cagggcgatg
     9121 ccgaagctca cgggcacggc gatgaagagg gctatggccg aggtggccag cgtgccgtag
     9181 atcatcacca ggccgccgta gtcgttctgc acggggtccc aggcgctgct cgtgagaaag
     9241 cccaggccat aggtggtgat ggagggccac gcccccacca cgagggaaat caatatgccc
     9301 agcagcgtgg ccagcgtgag caatgcggcc gatcgtgcga gccaggcgaa gaggcggtcg
     9361 gccatgctgt tggagagcac cggcccgcgc gcggggggca gggtctggcc ggagccggac
     9421 tgccgcaccg gctgcggcat ggggggagcg gtcagcgcaa tggacacggg aatgcctttg
     9481 tggtcttgca tggtgcggcg gcgggcacga tgggggcgcc cgccgcccgc ggattatttc
     9541 agtctgtgct ttgtctttac tccctccccc tttgggggag ggcaggggtg ggggccagcg
     9601 gcgcttggct gaaagctgtc gttcaggccg cggcccgaaa gcccccatcc ccgccttccc
     9661 ccagaggggg aaggagccat gccaatagcg cctacttcag cgccaccggt gccatcggtg
     9721 cgaccggctt gcccgcgccg tccttgatct ggccccagga cttgtagatc agctccttga
     9781 cgctggcggg catgggcaca tagtccagcg cgtcggccgt ggcgtcgccg ttctggtagg
     9841 cccattcgaa gaacttgagc accgtggccg cctgcgccgg cttgtcctgg ttcttgtgca
     9901 tcaggatgta ggtggcgctg gtgatgggcc aggaggcatc gccgggcttg ttgttgagca
     9961 cctggtagaa cgagcgcgac cagtccgcgc ccgcggcagc ggccttgaag ttggcgtcgc
    10021 ccgggctcac gaacttgccg gccgcgttct gcagctgcac gtaggtcatc ttgttctgct
    10081 tgacgtaggc gtactccaca tagccgatgg agttgggcag gcggcccacg aaggcggcca
    10141 cgccctcgtt gcccttgccg cccgcgcccg tgggccagtt gacggccgtg ccctcgccga
    10201 ccttggcctt ccactcgggg ttgacctggc tcaggtagtt ggtgaaggcg ttggtcgtgc
    10261 ccgagccgtc ggcgcggcgc acgggggcta tcgttgcgtc gggcagcttg acgccggggt
    10321 tgagcgcggt gatcgccggg tcattccact tggtgatctt gcccaggtag atgtcgccga
    10381 gcacggcgcc cgagagcttg agctcgccgg ccttgatgcc cgcgatgttg accaccggaa
    10441 tcacgccacc gaccacggtg gggaactgca gcaggccctt cttggccagt tcctcatcgg
    10501 tcagcggggc gtcggaggcg ccgaagtcca cggtcttggc atcgatctgc ttgacgcccg
    10561 cgctcgagcc cacagactgg tagttgatct tcacgccggt ggccttgtgg tagtcggccg
    10621 cccacttgga gtacagcggc gcgggaaagc tggcgccggc gccggtggcc tccagctggg
    10681 cccacgcggg agcgatgacg gcggccgcaa gcacgctgcc catcagcgcg cggattgcaa
    10741 gttgattcat tggttcagac cttcagagtg ttgaaacaga ccacgccttg aactctagaa
    10801 agcttgtgtg acggcgctgt gacagccctg acagccctga cagccctggc ggggctggtc
    10861 gcaccctggc cgccgcttgt ccgtgacgcc agaaggcctg ctggagcgaa ctttcggggt
    10921 tcgcgacgat caggggtaac atgacacgca tgttctttca ctccctgcct ccacccgctc
    10981 cggtcagcgg cggcgccgac ggtctgctgg cgcgcgtgct gggcgcgctg gtgtggctga
    11041 tgctggccgc gctgggggcg gtgtttgccc tcagtctgct gatctggctg gtggtgatgg
    11101 tggtggtcag cctggtgggc agcctgttca ccggccggcc ggccacggtg accctgctgt
    11161 ggcgccgcta ccgcgacatg acgcgcgagg tgacgcgcag ccgctggtcg ccgtcgcccg
    11221 ccagcagcac gccgcgcgcc gacgcggccc aggccaccgg cgccgccagt gcccagacgt
    11281 cggtgcagga cgtgtcgtgg cgcgaggtac cggccgcgcc ggccgccgac ggcgacgtgc
    11341 cggggcgctg aaacccctcc accgttgcta cattaacaat agctgcctgc gctttccggg
    11401 taagcgctgg atgccgatct gaccacgatt cggccggtca gaaggcgggc atgagaggcc
    11461 cgcccacagc tgttcaggcc tcgtggcgca gcgccgggaa caggatcacg tcacggatgc
    11521 tctggctgtc ggtcagcagc atcatgaagc ggtcgatgcc gacgccgcag ccgccggtgg
    11581 gcggcatgcc gtattccagc gcgcggatga agtcctggtc atagaacatc gcctcttcgt
    11641 cgcccgcgtc cttggcgctg gcctgggcgg caaagcgcgc ggcctggtcc tcggcgtcgt
    11701 tcagctcggt gaagccgttg gccatctcgc ggccggtgat gtagagctcg aagcgctcgg
    11761 tcacctcggg ccgctggtcg ttggcgcgcg ccagcgggct gatctcggtc gggtgctcca
    11821 tgatgaaggt gggctgccac aggtgctgct ccacctcttc ctcgaagtac atcacctgca
    11881 ggctggccag gctgcgctgg ctcaggcggt ccttgtcctc gctcaggccg agctttttca
    11941 ggcgtcggat cagctcggcc gcatcgtcga cgtgggcgcc gatgtcggtg tgcgtgtgca
    12001 gcgcctgacg gatggtcatg cgctggaacg gctggctcag gtccaccgcc cgaccctggt
    12061 agctcagttg cagcgagccg gtggcctgtt gcgcggtgtc gcgcagcagc gcctcggtga
    12121 agtccatcag gtcctggtag ttccagtacg cggcgtagaa ctccatcatc gtgaactcgg
    12181 ggttgtgccg cacgctgatg ccttcgttgc gaaagctgcg gttgatctcg aacacgcgct
    12241 cgaagccgcc cacgatcagg cgcttcaagt acagctcggg cgcgatgcgc aggaacatct
    12301 gctggtccag cgcgttgtgg tgcgtcacga agggcttggc gttggcgccg ccggggatgg
    12361 ggtggagcat gggcgtttcc acctccatga agccgtgccc ggtcatgaac tggcgcatcg
    12421 ccgacagcgt ggcgctgcgg gcagcaaagc gcgcgcgcgc ctgctcgtcc atgatcaggt
    12481 cgacgtagcg ctggcggtac ttcagctcct ggtcctgcac gccgtggaac ttgtcgggca
    12541 gcgggcgcag gctcttggtc agcaggcgga tgcgcgtggc gtggatcgac agctcgccgg
    12601 tgcgggtctt gaacaggtgg ccctcgcagg ccacgatgtc gcccaggtcc cagtgcttga
    12661 aggcctcgta atcggcctcg ccgacggctt cgcgcgcgac gtagatctgg atgcggccgg
    12721 tggcgtcctg caaggtggca aaactggcct tgcccatcac gcgcttgagc atcatgcgcc
    12781 cggccacgct ggcgtgccgc tggcggccgg ccgccaggtc ggcctcgaat acttcctttt
    12841 cgatagcgcc gtgcgcttgc tggagctgcg aggcgcggtc ttttggcttg aagtcattgg
    12901 gaaaggccac ggccccgccg gcggcctgca cggcgcggat ggcgcgcagc ttgtcgcgcc
    12961 gctcggcgat cagcttgttt tcgtcgtgtt cggaagcggc ggcttgcagg tcggcgggcg
    13021 ctgcgggctg gttcatggcg tgtcgggcag ggctggggcg cgagcagggc atgggcggca
    13081 gcgataattc gccgcgaccc ttgccgatgt cgagggaaac tgtctatttt aggcgcgcgc
    13141 cgcccgtgac cctgccgctg acccgtttcc gcccgtcgac cccgcgcacc cctgccggcg
    13201 cctgcccgcg ggcgccggcg ggcctgggag cgccgcggtg aaggccgcct ccggctcgca
    13261 gccgtcgctg acccgggcca cgctcaccct gttgggcggc ggcgcgctgg cgcagctgct
    13321 gccgctgctg ctggggccgt tcatcgcccg cctgttcacg cccgaagcgt tcggcctgta
    13381 cacccagttt gccaccgtgg cggcggcgct ggcggtggcc gccagtctgc gctatgagca
    13441 ggcgctgccg ctggcacgca gcgacgacga ggcgcgcgcg ctgctggcgc tggcgctgcg
    13501 actgctgctg gcggcggtgc tgctgggcgt gccgctgggc tgggcgctgc acgcgctggg
    13561 cgccttgccg ctggcggtgc tgctggcgcc ggcgctggcc acggccggcc tgctgcaggt
    13621 gctgatgctg tgggccaatc gggcccagcg tttcggtgcc ctggccacca gccgggtgct
    13681 gcactacggc ggcgcggcgg cgctgcaact ggtgctgggc tgggccctgt ggcgcggcgc
    13741 ggccagcggg gccgagggcg cctgggcgct ggcgctggct ccgctgctgg cgcagctgct
    13801 ggcctgcggc gggctgtggc ggcccgcgcc ggccggcggc tggcgggcgc tgctggtgcc
    13861 ggcgtcggcc gcgctgcgca cgcagatgcg gacggtggcg cggcagcagc gcgactttgc
    13921 tctcatcaac acgccgcacg ccctgctggg cctgctgcag gacgcgctgg cggtggcgct
    13981 gctggtggcc tggtcgggcg aggcggcggc cggcttctgg ggcctggcgc tgcgctacct
    14041 gaaggcgccg gccacgctgg tggggggggc ggtgtcgcag gcgctgtacc cgcgcctggc
    14101 ggcggcccgg gccgacgacg cgcgtcgcct ggtgcgccgg ctgatggccc tgctgggggc
    14161 gctggccctg gggctgatgg cgctgctgct gctggccggg ccgtggctgt tcgagcgcgt
    14221 gttcggcccg ccctggcgcg aggcgggcga gctggcgcgt gcgctggcgc cctacatcgc
    14281 ggcgcatttc gtggccgcgc cgctggcggt ggtgaccatg gcctggcgcg cccagcgctg
    14341 ggcgtttcgg ctggcgctgc tgggtcagct ggcgcttctg gccgcgctgg cgctgggtct
    14401 gcgccagggg gggctgctgg gcggcgcctg ggcggtttcg gcagtgatgc tgccgtactt
    14461 cggctggtac ttctggcgcc tggccggctg gcgcgacatt ccatcgcccc cgggcgtgcc
    14521 gccgcgcgag ggtgccccat gatcgcgctg cgtgccgcgc tcaaccgtct gcgccgccgg
    14581 ctgtggcggc tgctgtttca ccgcatgctg ttcggcgaag ccagtgccgg cgtgctgcgc
    14641 ccggccacgc gactgtcgcc cacggtgtgc atcgagcacg agccgacgct gacgctggcc
    14701 gacgacgtgt tcatcggcca tttctgcttc atcgaggcca gtggcggcgt gcgcatcggt
    14761 ggcggcacgc aggtgaccaa cttcgtcagc atcgtcagcc acagcacgca ccgcgcgctg
    14821 cgggtggcgg ccgggttgtc gggcgcgccg ctggaggcgg cggtgcggcg cgcgccgatc
    14881 gtgatcggtg aacgctgctt catcggcccg cacagcacca tcgaggccgg caccacgctc
    14941 ggccacggca cgctggtggc ggcgcacagc cgggtgcgtg gggtgtttcc ggacttcgcc
    15001 gtgctggccg gcagtccggc gcaggtggtg ggcgacacgc gcgcggccga cgagcgctgg
    15061 ctggcggccc atcccgaact gctggcggcg tatcggcgct ggcgccaggg gctggcgcag
    15121 tgacggcgcc gcatctgggc cttctgggcg acgccagcag cgtgcacgtg cagcgctggg
    15181 cgcgtgagat gcgcgcgcgc ggctggcgcg tgtcgctggt gacggcgcgg ccggcgccca
    15241 tcgagggcgt gtcgcagatc gtgttgcgcc cggtgcggcg cgccagcgac tggctgtggc
    15301 gcgtgggcga ggcgcggcgc gcgctgaccc agttgcagcc cgacctggtg catgcgcact
    15361 acgtcacctc ctacggctac ctggcggcgc gcgccggcct gcaaccgctg gtgctcacgg
    15421 cctggggcag tgacctgctg gtcacgccgc gacagagcgc gctcaagcgc tggctgacgg
    15481 cctggagcct gcgccgcgca cgcctggtga cgggcgactc gcgcgacttg ctggctgcgg
    15541 cgcgggcgct ggcgccgggt gtgccgaccc agctggtgca ctggggcgtg gagcgcagcc
    15601 gcttcgcacc cgtgccctgg gccgacaagc cggcggccga ggccgtgagc ccgcgcaact
    15661 gggagcccaa ctaccgcatc gacgtcgtgc tgcgcgcgct ggcgcaggtg cgcgccgtgc
    15721 gcccggaagc gcgcctgcac ctgctgggcg gtggcccgct ggaggccgat ctgcgcgccc
    15781 tgaccggaca gctggggctg caggacgcgg tgcgcctgca cggccggctc gacgacgccg
    15841 gcatggccgc ggtgctggcg cgctgcaagc tgtcgatcag cgtgcccgag agcgacgcca
    15901 cctcggtgtc ggtgctggag agcatggcct gcggcctggc ggtgatcgcc agcgacctgc
    15961 cggccaaccg cgactggctg gcgcccgagg cgctggtggc ggccggcgac gtggcggcgc
    16021 tggcggcgcg ctggctggcg ctgctgcagg acgacggccg cgccgcggcg ctgggcgcgg
    16081 ccaacgccga acgcatcgcg cgcgacgggg atcggcgcgt gcagatggac gccatcgacg
    16141 ccgcctgccg gcgcctgctg caggaggccg gccgatgacg caggatctgg ccacgccgcc
    16201 gctgccgcag ctgctgacgc cgggcgaggc cgggctggtg agcgtggtgg caccgtgccg
    16261 caacgagcgg gcccacatcg acgcgttctg cgactcggcg ctggcccagc gcctgcccga
    16321 aggctggcgc atggagctgc tggtggccga cggcgccagc gacgacggca cgcgcgcgcg
    16381 gctggccgag cgcgccgccg ccgatgcccg gctgcgcctg ctggacaacc cgcggcgcat
    16441 cgtctccagc ggtctgaacg cctgcctggc gcaggcgcgc gggcaggtga tcgtgcgcat
    16501 ggacatccac acccggttcg cccccgacta tgtcgccgaa tgcatcgccg cgctggcgcg
    16561 cagcggtgcc gacaacgtgg gcggcccctg ggtggcgcgc gggcagggcg gctgggggcg
    16621 ggccatcgcg gcggcgtttc agagccgctg ggtggtgggc ggggcgcgct cgcgcgatgc
    16681 ggcgttcgaa ggcgaggtcg ataccgtcta cctgggctgc tggccacgcg cgagcctggc
    16741 gcgcgtcggc ggctttgacg aaaccctggt gcgcaaccag gacgacgagc acaacctgcg
    16801 cctgcggctg gccggtggtc gcatctggca gagccggcgc attcactcca gctaccggcc
    16861 gcgcgattcg ctgcgccagc tcttcgagca gcagatgcaa tacggctact ggcgcccctt
    16921 cgtgctgcgc aagcaccggc aggcgggctc ggtgcggcag ctcgttccta tggtttttgt
    16981 agctgctggc gctttctgcg cgctgcttgc tccggtgttt gatcttggtc tgaaggcctg
    17041 ggccgcggcc tatgccgcct acctgctggc ggccagcgcg caggcggcgc accaggccgg
    17101 cgaggcgcgt ctggcgtggc ggctgccgtg ggtgatcgcc gcctaccacc tgggctatgg
    17161 cctgggcagc tggcgcggcg tctggggcct gtggcgccgg cgcgccgccc cgcaggcggc
    17221 gacccgcctg acacgctgac acaggcgccg tggcgcgcag cgcagacaat agcggcttca
    17281 tgagcgtttc cgaaagcttt cttccctttg cccgccccga catcggtgag gccgaaatcg
    17341 acgccgtggc gcgcgcgctg cgctccggct gggtcaccac cgggcccgag acgcgcgcct
    17401 tcgagcagga gttcgccgct ttcctgggcg gcggcgtgca ggcgatcgcc gtcaattccg
    17461 ccaccgccgg cctgcacctg gcgctggagg cgatcggcat cggcccgggc gacgaggtga
    17521 tcgcccccac gctgaccttc accgccaccg tcgaggtggc gcgctacctg ggcgccgacg
    17581 cggtgctggt ggacgtcgat ccggtcaccc tgaacatcga tccggccgcc atcgaggccg
    17641 ccatcacgcc gcgcacgcgc gccatcctgc cggtgcacta cggcggcctg gcctgcgaca
    17701 tggcggccat tcacgcgatc gcgcgtcgct acggcctgca ggtggtcgag gacgcggcgc
    17761 acgcgctgcc cgccagctgc ggcggcacgc cggtggggca gttgcctagc gcggcaacgg
    17821 tgttcagctt ctacgccaac aagaccatga ccaccggcga gggcggcatg gtggtgacgc
    17881 gcgacgaagc gctggccgcg cgcatgcgcg tgatgcgcct gcacggcatc agccgcgacg
    17941 ccttcgaccg cttcaccagc cgcacgccgg cctggtatta cgaggtggtg gcgccgggct
    18001 tcaagtacaa cctgaccgac ctcgccgccg cgctcgggcg cgtgcaactg gcgcgcctgc
    18061 cggccttcgt gcaacggcgc cagcagctgg cggcacgtta cctgcgcgag ctggccgggc
    18121 tgccgctggt gctgccgccc caggcgccgg ccggcgacac gcacgcctgg cacctgttcg
    18181 tgctgcgcct ggccgacacc gcgcgggtga cgcgcgacga ggtggtgcag gcgctggcgg
    18241 acgccggcat cggcaccagc gtgcatttcg tgccgctgca ccgccagccc tactggcgcg
    18301 accgctatgc gctcaggccg caggattttc cggtggccga ggcggcctac cagcgcatgc
    18361 tcagcgtgcc tctgttcacc gccatgagcg atgcggagca ggggcgcgtg atcgcggcgc
    18421 tgagggcctg cctgcaatga cgccggcccg cctggctgct tcgccctggc cctggtttgg
    18481 ctggagcgcg cgcgcctggg cgcggccctg gcgtgcgttc gtgcgcgact tcgccggctg
    18541 gcagggcgag tgcgggctgg agctcggcgc cggtccgcgc tcggcgctgg cgccgttgat
    18601 gctgagcctg gtgcggcagg tggaatgctc ggcccaggac gcggccagcc tgccggtcat
    18661 cgaggcgcgc caccgcgacc ttctggcacc gcaggacgcc gcccgcatcg cccacggcgt
    18721 gcgagatctg cgggcgctgc acgggcaatg ggacctgatc gtcatgaagt cggtgctggg
    18781 cggcgtgcac cggctgggcc gctcctcgct ggccgacgtg cacaccaccc tggagcaact
    18841 ggtggcccgg cacctgcgcc cgggcggcct gctggtgacg ctggacaacg ggcgcaccgc
    18901 gctggagccg ctgtggtcgc gctttggcgc gcgccgcaac ggctggcgct ttttcacgcg
    18961 cgacgagttc ccgccggcga cggcggcgta cggcttcggc ctgctgggca gcttctcggc
    19021 cgccacccgc ctgggtcggc cggggcgctg gagcgacgac gcgctgtacg gcgccgatct
    19081 gttgctgtcg ccctgggtgc ggcgccacgc cgtgctgctg cacgtctacc gcaagccggc
    19141 atgagcacca cgagcaccga cgccgccaag cgcgtgttcg acctggccct ggcgttgccg
    19201 gccctgctgc tggcgctgcc gctgatggcc gccatcgcgc tgtgggtgcg gctcgactcg
    19261 cccgggccgg cgtggttccg acaggcgcgt gtggggcgcg gcgggcggct gtttcgcatc
    19321 cacaagttcc gcaccatgca ggtgcaggcc gtggccggcg ggcccctgat caccgtggcc
    19381 ggcgacgccc gcgtgacgcg ggccgggcgc tggctgcgac gcaccaagct ggatgagctg
    19441 ccgcagttgc tggatgtggt tcgcggcgac atgagcctgg tcggcccgcg ccccgaggtg
    19501 ccgcgctaca tggcgctgta cccggacgag gcgcggcgcc tgatcctgtc ggtgcgccct
    19561 ggcctgaccg accgggcgtc gatcgaattt cgggacgaag agcggctgct ggccgccgcc
    19621 agcgatcccg agcgtgccta cgtcgagcag gtcatgccga tcaagcagcg ctactacctg
    19681 gactacgtgg cgcagcgcag cctgaccggc gatgtgggca ttctgtggca cacgctgcgg
    19741 gccgtgctgc ggcgtccgga ttccagatga aatcggcctt tgacgcccgt ctgtcatgcg
    19801 ccgcaagcta taaaaagaat agtatccgga agcctcagcg gggcgcgccg ccgatgccgg
    19861 tgcggctcag cagatcgacc accagctcca gccgcagcag gggactgtcc aggctcatca
    19921 ggtgctgacg cgtggcggtg ggcagcggca gcagctcgca ccagcggttg gccacccagc
    19981 cgcagtcgtc gtagtccagc gcgtcgccgc cgggcagccg caggccctgg ggttgctggc
    20041 gcagggtgtc gtgcgcgcgg cgcagggtgc gggcggcggc ctgcaggtcg tccggaatgg
    20101 tcaccaccgg gtcgggcggc agcaggctga cgtcggccac ccacaggcca tggcgcagct
    20161 ggtgcggctg gtcgatgcga aagcgctgtt cgccctggca ttcgatcagc atcagccccg
    20221 gctgcggttg cgtcagcgtg cgcacatgcg ccagcgtgcc gaccggctgc agccgctcct
    20281 gcggtgcgcc cggggtgcgc acttcctgac cctgcgccag cgccaccacg ccgaacggcg
    20341 cgccggcgcg ctggcagcgc ccgatcatgt ccaggtagcg cacctcgaac acccgcagcg
    20401 gcagccagcc gcccggaaac agcacggtgt cgagcggaaa cagcggcagg ctggtgaggg
    20461 tgagcggagc gtcggccatg gggcagggtg gtcgaccggc gccgcggggc gggcggacgg
    20521 cggccgctat catcgcacgc ggggatcggc gtgcgatggg cctggacgcc aggccgtgcc
    20581 gatcccgtgg ccgtcgcccc gcatgctcta cacgatcttt tcctttctgc tcgaaatcgg
    20641 cgtcaccctg gtcgggggcg cctgcctgct gcggctttac atgcgctggc ggcgcatgag
    20701 cttcgacaac ccggtgggcc agttcgtgct ggcgctgtcg gactggctgg tgcagccgct
    20761 gcagcgcgtg gtgccgcccg ccggcgggct ggacgtggcc agtctgctgg ctgcctggct
    20821 gctcaagctg gtgcagtacg cgctgctgat ggcgctgctg gagctgccgc gctggggtct
    20881 gctgccggtg ctggcgctgc tgggcacggc ccggctggcg gtgtcggtgt cgacggcgct
    20941 ggtgatcatc gcggccgtgc tgtcgtggac cggccagcgc agccgggcgc acgacgtgtt
    21001 cgagcgcctg tgcgcgccgc tgctggcgcc gatccgccgc cgcatccggc tggtcggcgg
    21061 ggtggatctg tcgccgctgc tgctgatcgt gctgctgcag gtgctgggca tcgtgctcag
    21121 ctcgttgcag tcgggcctgc tgggtccggg ggtgccggcg ctggctggct gatgcggctg
    21181 cgcgttcaca tgcatgaccc aagggtcatt ttgaacgcga gctcgcatga ggcgctgccc
    21241 gctcggccct gcggcccgct ggccggttct gccaccaccc cgaattgcta tcaatgtgat
    21301 agctttcggc gttgcaccca tgggcgccgc agcctgtttt tttactcaac ggcgtcggcc
    21361 ggctggcgct gcagcatcgc caggatggag gccaccttct ggcgcagttc gcgccggtcg
    21421 cagatgaaat cgaccgcgcc tttctgctgc aggaattcgg cacgctgaaa gccttcgggc
    21481 agggtcacgc gcacggtgtt ttcgatcacg cgcgggccgg caaagccgat cagcgccttg
    21541 ggctcggcga tcaccacgtc gcccacgaag gcaaagccgg ccgagacgcc gcccatggtc
    21601 gggtcggtca gcacgctgat gtagggcagg cccttcttgg ccaggcgggt gagcgcggcg
    21661 ttggtcttgg ccatctgcat caggctgaac aggccctcct gcatgcgcgc gccgccggtg
    21721 gcggtaaagc agatgaaggg cactttctgc tcgatcgcag tctgcacgcc gcgcacgaag
    21781 cgttcgccca ccacgctgcc catggagccg cccatgaagt cgaattcgaa cgccgccgcc
    21841 accacgctga tgctcctgac ggcgccgccc atgacgacca gggcgtcggt ttcgccggtg
    21901 ttttccagcg cctccttcag gcgctcgggg tacttgcggc tgtccttgaa cttcagggcg
    21961 tcgaccggca ccacctcctg cccgatctcg tagcgaccct cgccgtcgag gaaggcgttc
    22021 aggcgctcgc gcgcgccgat gcggtggtgg tggccgcagt gcgggcagac gttctggttc
    22081 tgctccaggt cggtcttgta gaggacggtg tcgcagtccg ggcacttgat ccacagcccc
    22141 tcgggcatct ggcgccgctc ggcggcgtcg cttttctgga tcttgggcgg gaggagtttt
    22201 tcgagccagc tcatggcgct ttccttcttg agtggcgccg attatgcgtc cagcgcggcg
    22261 cgcatcgggc gcagaaaatc ggccgcggcg gcggccacgc gctcgtgtgg ctggttttcg
    22321 atctgctgga tcagccggct gccgatcacc accgcgtcgg ccacctgacc gatggcgcgt
    22381 gcggtctcgg cgtcgcggat gccgaaaccg acaccgaccg gaatgtggac gtgctggcgg
    22441 atgcgcggca gcatggcgcc cacctcggcg gtgtcgagcg cgcccgagcc ggtgacgccc
    22501 ttgagcgaga cgtagtacac gtagccgctg gcgacttcgg ccacctggcg catgcgcgct
    22561 tcggtggagg tgggcgccag caggaagatc aggtccagcc cggcggcgcg caggcgggcg
    22621 gcgaagtcac ggcattcctc gggcgggtag tcgacgatca gcacgccatc gacgccggcg
    22681 gccgccgcat cgcggatgaa ggcgccatcg ccgtgcttct ggtcgtagcg ttcgaccggg
    22741 ttggcgtagc ccatcagcac caccggggtg tcgccgtcct tggcgcgaaa gctgcgcacc
    22801 atggccagca cctggcccag gccgatgccc agcgccaggg cacgttcgcc cgccttctgg
    22861 atcaccgcgc cgtcggcgct ggggtcggaa aacggcaccc ccagctcgat cacgtcggcg
    22921 ccggcggcca ccatggcgtg catcacggcg ggcgtgatgt cggcgtacgg aaaaccggcc
    22981 gtgacgtagg gaatgagggc cttgcggccc cgggcctgca gggcttcgaa ggtggcggcg
    23041 atgcggctca tgccctggcc cccttcacca cgtggccgcg catggagggg cggtcgtaga
    23101 agtcggcgcc gtcgagatcg gccacggtcc cgatgtcctt gtcgccccgg cccgacaggc
    23161 acaccagaat ggattggtcg ggtcgcatgg tgggcgccag cttcatggcg tgggcgaagg
    23221 cgtggctgga ctcgagcgcc gggatgatgc cctcggtgcg gcacaggtgg tgaaaggccg
    23281 ccagcgcctc ggcgtcggtg gcgccgacgt attcggcgcg gccggtgtct ttcagccagg
    23341 cgtgctcggg gccgacgccg gggtagtcca ggccggccga gatgctgtgc gtctcggtga
    23401 tctggccgtt gtcgtcctgc aggatatagg tgcggttgcc gtgcagcacg ccggggctgc
    23461 cgcgctgcag gctggccgag tgcttgccac tgtcgaggcc ctggccggcg gcttcgacgc
    23521 cgatcaggcg cgtgttttcg aacgggatgt aggggtgaaa aatgcccatg gcgttgctgc
    23581 cgccgcccac gcaggcgagc acggcgtcgg gttgccgggc cgccatgtgc tgctcggcca
    23641 gcagctcggg catctgcgtc aggcattcct ggccgatcac gctctggaag tcacgcacca
    23701 tcatggggta ggggtggggg ccggcgacgg tgccgatgat gtagaaggtg tggtccacgt
    23761 tggccaccca gtcgcgcatg gcctcgttca gcgcgtcctt gagcgtgcgg ctgccgcttt
    23821 ccaccggcac cacggtggcg cccagcagct tcatgcggta gacgttgggg ctctggcgct
    23881 tgacgtcttc gctgcccatg tagaccacgc actgcagccc atagcgcgcg cagatggtgg
    23941 ccgtggccac gccgtgctgg ccggcgccgg tttcggcaat gatgcgcggc ttgcccatgc
    24001 ggcgcgccag catggcctgg ccgatgacgt tgttgatctt gtgcgcgccg gtgtggttga
    24061 ggtcttcgcg cttgaggtag atctgcgcgc cccccagctc acggctggtg cgcgcggcgt
    24121 ggtagatcgg cgaggggcgg cccacgtagt gcgccagctc gtggcggaat tcgcgctgga
    24181 attccgggtc atgctggtac ctggcgtagg cgtccctcag ctcgttgatg gcgtaggtca
    24241 gcgtctcgct gatgaagctg ccgccgtagc ggccgaaatg cccgctggcg tcgggctggt
    24301 ggtagtcact catgtcgggg gcgatcctgc aagggcggcg tcggccgcac gcacggcggc
    24361 gacgaactga aaaatgcggg cagcgtcctt gatgcccttc aggggctggc cgtcggggcc
    24421 gacggcttcg acgccgctgg agacgtcaac cgcgagcgag cgcccgcggc cgcgcagcag
    24481 ccgcaggcca tcgcccacat ttgcaggtgt caacccacca gacaagacga ggtgagcggc
    24541 gacgtttgga ggcagcagtg accaatcgaa tgtctttccg ccgccgccga agccctcgac
    24601 atgggcgtcg agcagcaggg cctgtgccgt ggcgaaggcg tgggcaaatt ctatcaagtc
    24661 gaacgcgccg gcggcgcccg cagggatgcg cgccgcgcgc cagtacgggc gcccgcagcg
    24721 ggccgccacc cgggcgcagt cggcggctga ttcatcacca tgaaattgga gtgtagcgcc
    24781 cgtcaggagc gcgcaatcag ctatgatttc tgaagcatcg gcgttgacgt agagcagcac
    24841 cggcgtgacc atcggcggca accggcgcgc cagttcggcg gcgcgccgga tgtcgacgtg
    24901 gcgcgggctc ctggggtagc gcacgaagcc gatcgcatcc actcccgcgg ccacggcggc
    24961 gtccacgtcc tgcgcgcgcg tcaggccgca gatcttgatg cgggtgcgcg ccccggcgag
    25021 cgcaagggaa acgggtgtca tgatggcagc caatcatagg ccggcgtgcg ctcgggcaag
    25081 ccccagcggg cgtcgtagcg cggaccgagg aaatacagtc cgtcgggcgc aaacgtgggc
    25141 gccgccgcat cgcggctgcg cgcggccagc acgctgtcga tccactcggg cgggtgcagg
    25201 ccctggccga tcgccaccag acagcccatc aggttgcgca ccatgtggtg caggaaggcg
    25261 ttggcctcga agtcgaagcg ccagtaggcg ccgcagcggc ggatgtcaat gcgctgcagc
    25321 tgcttgacgg gggtcagcgc ctggcaggcg ctggcgcgaa acgacgagaa gtcatgttcg
    25381 ccgatgagtc ggtcggccgc gcgccgcatc gccacgccat ccaggggccg gaaggcccag
    25441 ccgacgcgcc cgctctccag gctggggcgc accggcgctt ccagcacgac gtaggcgtag
    25501 cggcgcgcca gcgcgctggc gcgcgcatga aactcctgcg gcaccggctg cgcccactgc
    25561 acggcgatgt cgcgcggcag aaagcggttg gtgccgcgca cccaggagaa agcatcgcgc
    25621 tcaagctcgg tgtcgaaatg caccacctgc atcaggccat gcacgccggc gtcggtgcgg
    25681 ccggcgcaca gcgtggcgat gggggaggtg gcaaatgcgg tcagcgccgc ctccagccgg
    25741 tcctgcacgg tctgcccggt gggttggctt tgccagccct ggtagggggt gccgagatag
    25801 ctgaggccga gggcgattct cacgatggcg tgattgtgga cgaagcgtgg gctccaggag
    25861 ggctccaaaa gggcgcggcg cccatcgggg cgccgcgttg ccggagtccg gaggccaggc
    25921 ctcggtgggg acggatcagt cgagctcggc caggaaccgc tcggcccgca tgcgcaggct
    25981 gccggtggcc tcggcgatca cttccctgac cagcgtgcgc gcgccctcgg tatcgccgat
    26041 ggcgtggaac tcctgcgcca gcgccagctt ggtggccacc ggatcgtcgt cggcatcgtc
    26101 cggttgttcg gttttcggat caaccccgga gcgagagtcc ggatcgatcg acagggcctc
    26161 catgtcgaag tcgatcatgc cagccttgct cgccacatag ggctgagcaa ccgatggcgg
    26221 ttcgtcgtcg gcctgcgtgg caaccgcggc agccagggcg ggggccaccg tggtcggcgt
    26281 gtctaggtcg aggtcgaagt cgggcagcgc aatcggcacg ggcttgactt cgggcgcggc
    26341 agcgatcgcg ggctccgggc cgtggtgact cggcatggcg ctggtgttcc ccacggacgg
    26401 ggggaccgac gtcaccgcgc tggcacgcac cggggcgtcg tccaggtcga gttccagatc
    26461 cagcgggatg ctcgagctgt cgggcttgga atcgcgcttg tcctgcaatt gcgccgtctg
    26521 tggctcggta tcggcaccga accgcggctg cggtgtagtg gcggtggcgg cgctccgcat
    26581 catgggaaca ccgcccggct tgtagagggg gttgtcggga tcgagttcgc tgccgaggct
    26641 ggcgattgcc gcccagtcgg caccttctcc cttgacgtgg ttccaggctt ccgaggcgac
    26701 catttcgagg gcgcgggcgt cgcgccgtct ggcatagatc tctgccagct tgcgatgcac
    26761 gctgacccgt ccaggatggg tgcgcagtgc ttctttgagg atttcctcgg cctgcatgtc
    26821 acgtccgtag gccaggtaga cgtcggcctc ggccacggga tcgacgtcgc cggctgcgtc
    26881 gagttgactg ggcgaataga ccatggacga gctgctgccc accgacggcc gaccctcgcg
    26941 cgtgtcgacg cgctgtccac cgctggcgcc gaagaaggag tcgggctgca aacggctctc
    27001 gatgaacgag ctgtccattg tggcgtcgcc cttcttgctc tggcgccagc ggtacacacc
    27061 gaagcccgcc agcagggcga tcaaacctgc accggcgata ggcagcagcg ggttgtcggt
    27121 cagcgcatcg aggaaagacg gctcctcact cgcggtggtc ggcggcacgg gagctgcctg
    27181 cggcgccgga gcggcaactg gcgctgtcac cgtttcggag acgccggaag ccggaaccgc
    27241 tgcaggttct gccatggcgg cggaggcggg tgcagccggg acggccgaag ccgcatcggc
    27301 cattgccggt gccggtgccg gcactgaagc gacgctggcc ggttcggagg cgctggctgg
    27361 cgtcgcaacg gtgggcgccg ccggttcggc ggcgacggaa gccgcaggtg cggcgggcgt
    27421 cgctgcgacg ggtgcggctg cgggcggagt gccgatgggc aatttcacgc ccgtcgaagt
    27481 cgcgctgccc gatgcggcct gcaggcgcgc cagctcgctg agattcttgt tcaactcggc
    27541 gacgcgatcg gattgctcct gagcctggcg ggactgcgcc agttgtgact ccgctcccgt
    27601 ttgtgcgccc tcgcgcgaca gcttcaggcg atcggggctg ggcgccgccg cgcggctgtc
    27661 ccggacttca ggttgcaccc caccggacgc actgccagtg gcggccgtcg ccacgcgcgt
    27721 ggctccgctg cgctgcgcca ggccaagacg gtaggcgttg aagtcccgcg tctgggcaac
    27781 gatggcacga cgggcggctt gccgggtgat tgcgcctgcc tgctcgaccg acggcatctg
    27841 gagcacggtg cctgcccgca gcaaattgac gttgccgttg atgaacgcat cggggttggt
    27901 gcgcagcatg gcgaccagca tctgatcaag cgacacgccc tcgatggcat gcgcccgcgc
    27961 gatgctgcct gcggtatcgc cacgctgcac cgtcacgttg gcgccttcac cggtggagct
    28021 caccgcctgt cgtggcgcct cggcgggatg gatgtcgcgg cgtgggcgcg agcccgcgtc
    28081 cgccgccgcg acgggcggga ggggggccgc cctgggcgca tgcaccacgg cagatgtcgg
    28141 tggcgctgtg gcctgcgagg cggtgacgga gaccggtgga ggcgcaccac gacccggcgg
    28201 atccaccagc aaggtgtagt cgcgcactac gcggccattg gcccaattgg cctcgaccac
    28261 gatgctaagg aagggttcgt tgacggtgcg ttcaccagtc acccgcaagt aggcttgacc
    28321 gctggctcgc cgctgcaggc tcacgcgcgc cccggccagg gcggcgggga aatccactcc
    28381 ggcagcgcga aacgcttcgg gtgagccgat gttggccttc agcgtcgcgg cttcttcgga
    28441 agagatctgc ggcacctcga tctcggctct caggggctca ccgagtgccg agcggaccgt
    28501 gatggcaccc agcgccagcg cttgcgcgtc caggctcacg ccgcacgtgg caatggccac
    28561 ggccgaggcc agcacgcctt tgcgccaagg ttggcgaacg ctgtgacttg aagcaatcga
    28621 cccgagcaag gtcatagcgg tcttgttctg catgtctggt gttgggcagg ctgagcccgg
    28681 ccggaacgaa tgtggaaaac gacattaaca tcaacgtatt agcctgacaa gctgaggcac
    28741 tgtctaatcc tgcctgtagc gcctcactgc ggtgcaaggg cactgagctt gttgcaatac
    28801 gacaacgggc agcccccgtc cagggccgcc cgctgcgaga tccgcgccgc tcgtcagtcc
    28861 tgcagcagga tgcgcaacat gcggcgcagc ggctcggccg caccccacag cagctggtcg
    28921 ccgatggtga aggcgcccag gtattgcggc cccatggcca tcttgcggat gcgacccacc
    28981 gggatggtca gggtgccggt gacggccacc ggggtcagat cgcgcagggt ggcgtcgcgg
    29041 ttgttgggca ccactttcac ccagtcgttg tcggcggcga tcatggcttc gatgtcggcc
    29101 accggcacgt ccttcctgag cttgaaagtc agcgcctggc tgtggcagcg catggcgccg
    29161 atgcgcacgc agtagccgtc caccggcacc gccggcgtgt cgaagccttc gccctggccg
    29221 aggatcttgt tggtttcggc catgcccttc cattcttcac ggctcatgcc gttgcccaga
    29281 tccttgtcga tccaggggat cagcgagccg cccagcggca cgccgaagtt ggctttctcg
    29341 gcgtcgtcca gcgcgcgctg cttgccgatc accttgcgat cgatttccag aatggcgctg
    29401 gtcgggtcgg acagcaggtc gcgcacctcg tggttcaggg tgccgaactg cgccagcagc
    29461 tcgcgcatgt gctgcgcgcc accaccgctg gcggcctggt aggtctgggt gctcatccat
    29521 tcgaccaggc cggccttgta gagcgcgccc acgcccatca gcatgcagct gacggtgcag
    29581 ttgccaccga tccagtcgcg cccgcccttg gccagcgcgt tctggatcac cggcaggttg
    29641 accggatcga gcacgatgac ggcgttcttt tccatccgca gggccgaggc ggcgtcgatc
    29701 cagtggcccc cccagccggc ggcgcgcagc ttggggtaga ccgcatgggt gtagtcgcca
    29761 ccctggcagg tgaggatgat gtcgcacttt ttcagtgctt cgatgtcgtg cgcgtccttc
    29821 agctggcgtt cgtttctggc ctgggcgggc gcggcgccgc cggcgttgct ggtggaaaag
    29881 aagatgggct cgatcaggtc gaagtcccgc tcggcctgca tgcggtccat caggaccgag
    29941 ccgaccatgc cccgccagcc gacgaggcca acaacgttac cggttttcat ttgctttggc
    30001 gcctttcaaa caaagtggat ttgctctgac ctgccgggct cgtctggctc cggaggcggc
    30061 gcgcacgacg aaccgaaggc tgatcagcct tttttggtga tcgtggttgt gcggaacgtg
    30121 gacacgccag cggccggcgc ggtggccggg cggtgcagat cgcggggatc gggcgtgtgc
    30181 atggtgcgca gattctagcg caggccggcg ccgctgcggc gactgcccct gcgcggcacg
    30241 cggcacctgc ggcgtggggg cgacagtggc tggccggcgc ccgcgcctgc gcgcggaatc
    30301 atgaacaaaa tggcaactgg cgcttacgca gcaagcgcca gaagctattc aaatgatagc
    30361 ttaggaatgc ttttgcggtg ccggctgaac cccgttcggc ccctcgctga ccggcaccgc
    30421 ggcgcggcgg cgctcaggtg gtgggccggg cgatcttcag tccagcgccc ggaccacggc
    30481 ctcgcccatc tcgcgcgtgc ccacacgggt cgtgccttcg ctccagatgt cgggcgtgcg
    30541 caggccctgg gccagcacct tcctgacggc ggcttcgatg cggtcggcgg cttcgggctg
    30601 accgagcgag aagcgcagca tcatggcggc cgagaggatg gtggccagcg ggttggccac
    30661 gcccttgccg gcgatgtcgg gggcgctgcc gtggctgggc tcgtacaggc cctgcttttt
    30721 gtcgttcagg ctggccgagg gcagcatgcc gatcgagccg gtgagcatgg aggcttcgtc
    30781 cgacaggatg tcgccgaaca tgttgccggt gacgatgacg tcgaacgcct tgggcgcctt
    30841 gaccatctgc atggcggcgt tgtccacgta catgtgcgac agctccacgt ccgggtagtc
    30901 cttgtgcacc tcggtgacca cgtccttcca gagctggaag gtttccagca cgttggcctt
    30961 gtcgacgctg gtcacgcgcc gcgagcgctg acgtgccgcc tcgaaggcca cgcgggcgat
    31021 gcgctcgact tcggggcgcg tgtagcgcat ggtgtcgaag gcttcttcgg cgcccggaaa
    31081 gtgcccatcg ggggcctggc ggcggccgcg cggctggccg aagtagatgt cgccggtcag
    31141 ctcacggatg atcaggatgt cgagccccgc caccagttcg ggtttgaggc tgctggcggc
    31201 cgtgagctgc tcgtagcaga tcgcggggcg gaagttggcg aacaggccca ggtgcttgcg
    31261 caggcccagg atggcttgct cgggacgcag cggacgatcc agcgtgtcgt acttccagtc
    31321 gcccacggcg ccgaacaaaa tggcatcggc ggcccgggcc agattcaggg tggactcggg
    31381 cagcggatgg cccgcggcct cgtaggcggc gccaccgacg ggagcgcttt ccagctcgag
    31441 tttcaggccc agcgcctgca gcaccttgac ggcttcggag gtgatttcgg ggccgatgcc
    31501 gtcacccggc agaactgcga ttttcatgtg gcaaagactt cagaatggat agctgccggc
    31561 gcttgtctgg tgcgcgctgg cggccgattt ggcttggaag caaggggcgc ggccgcggcg
    31621 ccgcgccatc aggccgggcg gcccatggtg tgggccagcc agggtttgct ggccaggcgc
    31681 tcggcttcga agcgggcgat cgcgtccttg tgctgcagcg tcaggccgat gtcgtccagg
    31741 ccgcccagca ggcagtgctt gcggaagggg tcgatgtcga acgccagctc ggtgccgtcg
    31801 cccttgacga ccacctgccg ctgcaggtcg atggtgagct ggtagccggg aaaggccgcc
    31861 acgtcatgga acagcctgtc cacggccgac tcgggcagca cgatgggcag caggccgttc
    31921 ttgaagcagt tgttgaagaa gatgtcggca aagcttggcg ccaggatggc gcgaaagccg
    31981 tactgctgca gcgcccaggg ggcgtgctcg cggctcgatc cgcagccgaa gttctggcgc
    32041 gccagcagaa tgctggcccc ctggtagcgc ggctggttga gcacgaaatc ggggttgggt
    32101 ttgcgactgg ccgggtccat gccgggctcg ccgtggtcca ggtagcgcca ttcgtcgaac
    32161 aggttggggc cgaagccggt cttccggatc gatttcagga actgcttggg aatgatggcg
    32221 tcggtgtcga cgttctcgcg gtccagcggg gcgaccaggc ccttgtgggt ggtgaaagcg
    32281 tccatgtcgt gggttcgagg aaagggttgc tggggcgcgg gcagcggcgg cgcccgatga
    32341 tggacgggtg gaatgcttcg cccaagccac cgctggcgca cgcacggggc gtgcgcctgg
    32401 ggtggtcggg cgaatggcag cctggccatc gcccgccggg ggcgtgtggc cagtgcgggc
    32461 gggccgaacg tgggcttact tcttgccggc gttctcgacc gccgagccgg ccttctgcac
    32521 gtccttgccg acgccttgca cggtattgca accggccagc atcagggctg cggcaaccag
    32581 ggcgagtact tttttcatga tgagctcctt ctgggggtta agagatctgg cgaatgtcga
    32641 cgaaatggcc atgcacggcc gctgcggcgg ccatggccgg gctgaccagg tgggtgcgcc
    32701 cgccctggcc ctgacggcct tcgaaattgc ggttgctggt ggaggcgcag cgctcgcccg
    32761 gctccaggcg gtcggcgttc atggccagac acatgctgca gccgggctcg cgccactcga
    32821 agccggcggc ggtgaagatc ttgtccaggc cctcggcctc ggcctgctgc ttgaccagcc
    32881 ccgagccggg taccaccatg gccagcttca cgttcggcgc cagccggcga cccaggcgct
    32941 tgaccaccac tgcggcggcg cgcaggtctt cgatgcggct gttggtgcag ctgccgatga
    33001 acaccttgtc gacgtggatg tcggcgatcg gcttgccggg aatcagtccc atgtaggcca
    33061 gggcgcgttc catggcgccg cgctgcaccg ggtctttctc cttgtccggg tcgggcaccc
    33121 tggcgtcgac accgaccacc atttcggggt tggtgcccca ggtgacctgg ggcacgatgg
    33181 cttgggcgtc gagttcgacc acggtgtcga aatgggcgcc gtcatcgctg tgcaggctgc
    33241 gccagctggc gaccgcctgg tcccactcgg ggccaccgac gaagcggccg gtgacggggt
    33301 cggtgccggg ggccatgggg cggcccttga cgtagtcgat ggtcttgtcg tccaccgcga
    33361 tcaggccggc gcgcgcaccg gcttcgatgg ccatgttgca caccgtcata cggccttcca
    33421 tgctcaggtc gcggatcgct tggccggcga attcgatggt gtggccggtg ccgccggcgg
    33481 tgccgatcct gccgatgatg gccagcacga tgtccttggc ggtgatgccg ggtgccaccg
    33541 cgccgttcac gcgcaccagc atgttcctgg ccttcttggc gatcagcgtc tgcgtggcca
    33601 gcacgtgctc gacttcgctg gtgccgatgc cgtgcgccag cgcgccgaag gcgccgtggg
    33661 tgctggtgtg gctgtcgccg cagaccaccg tcatgccggg cagggtggcg ccgttttccg
    33721 ggccgatcac gtgcacgatg ccctggcgct ggttcatgaa cggaaagtag gcggcggcgc
    33781 cgtgggcctg catgttggcg tccagcgtga cgatctgctc cttgctgatc gggtcctcga
    33841 tgccgtcgta gccacgctcc cagccggtgg tgggggtgtt gtggtcggcc gtggcgacca
    33901 cgctgctggt gcgccagacc ttgcgcccgg cctgacgcag gccctcgaag gcctgcgggc
    33961 tggtgacttc gtgcaccagg tggcggtcga tgtagagcag ggcggtgccg tcctcttcgg
    34021 tgcggacgac gtgctcgtcc cagatcttgt cgtacagcgt tcgcggggtc atgtcggcca
    34081 ttctcgtgat tgtcaaattc tatgctgcgg ctgcgcgtcg tgggagccgc gcaggtgggc
    34141 tgtcagcctg gggcaggtgc caatgccggg ccgcgccgtc atggtcggcc gtgtgcgggg
    34201 tgcggggcag tccgaatcca tgttgtccga atggcggttg gcgcttgtgt ggcaagcgcc
    34261 agcagctatc gttttggtag tgttgtggcg gcggccatga aaaacccgcc acgcgggcgg
    34321 gtgctcggcg cgcggcgcgg cgcggcgcgg gctcagggca cgtcgcggcg cggcgagccg
    34381 gtgaagagct ggcgcgggcg gccgatcttg tactcggggt cggccagcat ttcgttgagc
    34441 tgggcgatcc agcccaccgt gcgggccagc gcgaagatgc cggtgaacag gctgacgggg
    34501 atgccgatgg cgcgctgcac gatgccggag tagaagtcca cgttcgggta gagcttgcgc
    34561 tgcacgaagt agtcgtcctc gagggcgatc ttctcgacct gcttggccag ggcgaacagc
    34621 gggtcctttt ccaggcccag ctcggccagc acctcgttgc aggtttcctg catgagcttg
    34681 gcgcgcgggt cgtagttctt gtagacgcgg tggccaaagc ccatgagctt gacgccgctg
    34741 ttcttgtcct tgacctgctc catgaactcg cccaccttgg cgatgccgcc gttgcgctgg
    34801 atgtcttcga gcatgttcag gcaggcctcg ttggcgccac cgtgggcggg gccccacagg
    34861 caggccacgc ccgcggcaat ggcggcaaac ggattcgtgc ccgaggagcc gcacaggcgc
    34921 accgtggagg tggaggcgtt ctgctcgtgg tcggcgtgca ggatgaagat gcggtccatc
    34981 gccttttcga gcaccgggtt gaccttgtag tcctcgcagg gcgtgccgaa catcatgcgc
    35041 aggaagttgc cggcgtagga cagctcgttg cgcgggtaca tgtagggctg accgacgccg
    35101 tacttgtagg ccatggccac cagcgtgggc agcttggaga tcaggcggat ggccgagatg
    35161 tggcggtgct ccgggttatg gatgtcggtg ctgtcgtggt agaaggccga cagcgccccg
    35221 atcaggccgg tcagcacggc catcgggtgg gcatcgcggc ggaaaccgcg caggaagaac
    35281 tgcatctgct cattcaccat ggtgtgcttg agcaccagct tgtggaaatc gctgcgctgg
    35341 gtggggttgg gcagttcgcc gttgagcagc aggtagcagg tgtccaggta gtcgcacttc
    35401 tgtgccagct gctcgatggg gtagccgcgg tacagcagtt cgcccttgtc gccgtcgatg
    35461 taggtgatgg cggactggca ggatgcggtg gacagaaaac ccgggtcgta ggtgaacatg
    35521 cccgtctggc cgtacagctt gcggatgtcg atgacgtccg gaccgatgct gccctggtag
    35581 accggcagtt cgatgtcggg gctgccgttg gagaaggaca gcttggcttt gttgtcggag
    35641 agtttcatgc tcggattcct tgaatggagg cagacgttga agcggagcga aaggcgcgtc
    35701 aggcggcagc aggacgcagc agggtcagca actgacggat gtcgggcgtg ttgaggtcgc
    35761 catcgggttc cttgcggcgc agcagcaggt cgagcagctc gttgtcggac aggtccatca
    35821 actgggtcag tgcggcggcc tggcttgccg tcagctgctc ggcgtggcgc tggaagaagc
    35881 gctcgatcag cagatcgttc tccagcatgc cgcgacggca gcgccacctg agcatgccga
    35941 gggctttctc gtcgatgcgt tcgctctcca tggttgcgcc ggcggatcgc agggtcgtca
    36001 gatgccccgg cgaaccagga gctccttgat cttgctgatg gccgcggccg gtttgaggcc
    36061 cttgggacac acatccacgc agttcatgat ggtgtggcag cggaacagtc ggtaggggtc
    36121 atccaggttg tccagccgtt cgccggtggc ctggtcgcgg ctgtcggcga tgaaacggta
    36181 ggcctgcagc aggccggccg ggccgacgaa cttgtccggg ttccaccaga agctcgggca
    36241 gctggtggag cagctggcgc acagaatgca ctcgtacagg ccgttgagct cctcgcgttc
    36301 ctcgggcgac tgcaggcgtt ccttggaggg cgaggtgggc tggtcgttct gcaggtaggg
    36361 cttgatcgag tggtactggt tgaagaactg cgtcatgtcc acgatcaggt cgcggatcac
    36421 cggcaggccg ggcaggggct tcagcacgat gtcgcccgac agcgtcttca tgttggtcag
    36481 gcaggccaga ccgttcttgc cattgatgtt catggcgtcc gagccgcaca cgccctcgcg
    36541 gcaggagcgg cggaacgaca gcgaagggtc ttgcgccttg agcttgagca gggcgtccag
    36601 cagcatgcgc tcgtggccgt cgagctcgac ctgcacggtc tgcatgtaag gcttggcgtc
    36661 cttgtccggg tcgtagcggt aaatcttgaa tgtgcgttgc atggtgtgtg tctttcgcgt
    36721 ggcagcgctc gggctcagaa cgtgcggacc ttgggcggga tgctgtcgac cgtcagcggc
    36781 ttcatgttca ccggcttgta gctcaggctg ttggtggcgc tgtcccacag cgtgtgcttg
    36841 agccagttgg cgtcgtcgcg gcccagcggg gcggtggggt ggtcggccgg gtgttcgtag
    36901 tcgtacaccg tgtgggcgcc gcggcattcg tgacgcgcgg cggccgagac catggtggcc
    36961 tgcgccacct cgatcaggtt gtcgacctcg agcgcttcca tgcgggcggt gttccagacc
    37021 ctggacttgt cattgagcgt gacctgtccg acccgctcgc ggatgccagc gatcttgacc
    37081 acgccttcgt ccatgccctt ctgcgtgcgg aacacgccgg catgctgctg catggtctgg
    37141 cggatttcgt tcgccagatc ctgcgcgtag atgccgtcgg tggagttttc gagctggttc
    37201 aggcgcgcca gcgtgcgctc gctggcgtcg gccggtaccg ccgcctgttc gccgtagctt
    37261 ctgacgaact gcacgatgtg tttgccggcc gacttgccga acaccagcag gtccaagagc
    37321 gagttggtgc ccaggcggtt ggcaccgtgc accgacaccg cggcacattc gcccacggcg
    37381 tacaggccgt tgacgatgtt gttcttctcg ccgtcccagg tcacgacctg gccgtggatg
    37441 ttggtcggga tgccgcccat ctggtagtgg atggtgggca cgacggggat gggctccttg
    37501 gtgatgtcca cgttggcgaa gttgtggccg atctcctcca ccgagggcag gcgcttacgg
    37561 atggtgtccg cgcccaggtg gtcgagcttc atcaggatgt agtccttgtt cggaccgcag
    37621 ccacggcctt ccttgatctc ctggtccatc gagcgggaca cgaagtcacg cggagccagg
    37681 tccttgagcg tgggcgcata gcgctccatg aagcgttcgc cgttgctgtt gagcaggatg
    37741 gcgccctcgc cgcggcagcc ttcggtcagc agcacgcccg cgccggccac gccggtgggg
    37801 tggaactgcc agaactccag atcctgcagc ggaatgccgg cgcgcgccgc catgcccaag
    37861 ccgtcgccgg tgttgatgaa ggcgttggtc gatgcggcaa agatgcggcc cgcaccgccc
    37921 gtggccagca gcacggcctt ggcgtgcagc tcgtacaggt cgccggtttc cagttcgagg
    37981 gccgtgacgc cgacgacgtc gccggcgctg ttgcggatca ggtccagcgc catccactcg
    38041 acgaagaagt tggtcttgga ctcgacgttc ttctggtaca gcgtgtgcag catggcgtgg
    38101 ccggtacggt cggccgcggc gcaggcgcgt tgcacgggct tttcgccgta gttggccgtg
    38161 tggccgccga aggggcgctg gtagatggtg ccgtcggggt tgcggtcgaa cggcatgccg
    38221 aaatgttcga gctcgatcac cacgttgggt gcttcgcggc acatgaactc gacggcatcc
    38281 tggtcggaca gccagtcacc gcccttgatg gtgtcgtaga agtggtagtg ccagttgtcc
    38341 tcgctcatgt tgcccagcga cgccgacacg ccgccctggg cggccacggt gtgcgagcgg
    38401 gtcgggaaga ctttggacag gcaggcgacg ttcaggccgg cgcgcgcgag ttcgagcgag
    38461 gcgcgcatgc cggagccgcc ggcgccgacg atcaccacgt cgaacttgcg ggtgatgact
    38521 tggtctttgg tgtaactcat ggtcgtggtt cagtgatgct tgtcatcggg tacggaacgc
    38581 ggcgctcaca gacgccacag cgcctgcagg gcccagccgc cgcaactcac cagccagatg
    38641 acggtgaacg tctgcagcag caggcgcacg ccggcggcct tgacgtagtc cagccagatg
    38701 ctgttcatgc cgatccaggc gtgccagatc agtgcgacga tgacggagaa ggtcaaggcc
    38761 ttcatccact gggcggcaaa gatgccggcc caggtgtcgt agccgatggg gccctgggtg
    38821 aacagcacct gcagcagcac gatcagcgtg aaggccgcga tcagcgcgcc gctgacgcgc
    38881 tgcaccagaa aatcgcgcca gccgtaatgg gcgccgacca ccagtcgctt gttgccgtaa
    38941 gtcgtggaca tgttgttgct cctggcaggt cagatcaata caggccaaac agcttggcgc
    39001 cgagggccag cgtgaggacg atgctcacca ccagcgtggc gacggccgac tgacggccga
    39061 actccttggt ggtgcgatgc gagatgtcga ggtacaggtg acgcacgccc gccagcaggt
    39121 gatgcaggta ggcccagatc agcgccagca ccaccagctt gacgaaccag gccggaaacg
    39181 cccccaggcc ggacgagaaa gcggcgctga actggccgaa cgaaatctcc gacgacacgg
    39241 atttgtcgaa cagccagatg atgaagggca gcagcagaaa catcagcgcc ccgctggctc
    39301 ggtgcaggat ggacacccag ccggccaggg gcaaccggta ggtgcgcacg tcgttgaaga
    39361 tgttgatgtt gcggaattcg ggccgcggct tggtggcttc gctcatggga actctcgtgg
    39421 gaagggattg cagagttgca attcttggag ggggagtgca aattctattg caatgcagca
    39481 agctgacggg acgattgcag gggagggtcg gcaggtcgat cgagcaggag catgagccgc
    39541 gtcaactcag cgtcaactca gtgtcaactc agtgcattgc ggtagtggtg ggtgtcggtg
    39601 cggtacaggc cgcggcgcag ttccatcggc tcgtcgttgt aggtgtaggc aatgcgttcg
    39661 acgctcagca acggggtggc gggcgtgacc tccagcagcg cggcctgcgt cgggtcgggc
    39721 agcactgcgc gcagcttttc gtcggcgcgc accatgcgca cgccgaactc ggcttcgaac
    39781 agggcgtaca tcgggccgtg gtactgcgac aggcgctcgg cggtcagtcc cttgaacgga
    39841 ccggcgggca gccacagatc ttcgaggatg gtcggcacac cggcaaagcg cagcaggcgg
    39901 cgcgcctgca gcaccggatc gcccgcgcgc agaccgagca ggcgcgccac gtcggcgctg
    39961 gcgcgctgtc gcttgcagtc gacgaagtcg cgctgggccg gcccttcgct gtcggccgtg
    40021 ccgcggtccg gttgcaggcg cagaaagcgg tactgcacct gttgctcggc gtgggtggcc
    40081 acaaaggtgc cacgcccctg gcggcgcacc agaaggttgt cggccgccag ctcgtccacc
    40141 gccttgcgca ccgtgccctg gctgaccttg tagcgcgcgg ccagatcgaa ctcgctcggg
    40201 atcacctcgc cgggcttcca ttcgccctgt tgcaggctct gcaggatcag gctcttgatc
    40261 tgctgataca gagggctgaa cgccggcgcc tgcccacccc aggcggtggc aggtgcaagc
    40321 gccggcgggg cgctttcggt cggggtggaa gagggtgctg cgtgggccat gtcgggtgtc
    40381 agtctgacgt aggactggat ggcgatgata tcttatataa gacacaagac aaattgacga
    40441 atcagggttt tcgcggatac acttggacgg tcgatttctc gtcctgcagg ggtgccagcc
    40501 gtggctgccc gcgccctgtg gtcagccact tttcaccaca accttctgga gttttcccat
    40561 gagcaagaag cccgttcgcg tcgccgtcac cggcgctgct ggtcagatcg gctacgccct
    40621 gctgtttcgc atcgcctcgg gcgaaatgct gggcaaggac cagccggtga tcctgcaact
    40681 gctggagatc ccggacgaga aggcgcagaa ggcgctcaag ggcgtgatga tggaactgga
    40741 agactgcgcc ttcccgctgc tggccggcat ggaagcgcac agcgacccga tgaccgcctt
    40801 caaggacacc gactacgcgc tgctggtggg tgcgcgtccg cgcggccccg gcatggagcg
    40861 cgccgacctg ctggccgcca acgcccagat cttcaccgcc cagggcaagg cgctgaacgc
    40921 cgtggccagc cgcaacgtca aggtgctggt ggtgggcaac ccggccaata ccaacgccta
    40981 catcgccatg aagagcgcgc ccgagctgcc ggccaggaac ttcaccgcca tgctgcgcct
    41041 ggaccacaac cgcgcggcca gccagatcgc tgccaagacc ggcaaggccg tcaaggacat
    41101 caagaagctc gccgtgtggg gcaaccactc gcccaccatg tacgccgatt accgcttcgc
    41161 caccatcgat ggcgccagcg tgaaggacat gatcaacgac caggaatgga acgccaacgt
    41221 gttcctgccc acggtgggca agcgtggcgc cgccatcatc gacgcacgcg gcctgtcgtc
    41281 ggccgccagt gccgccaacg ccgccatcga ccacatgcgt gactgggcgc tgggcacggg
    41341 cggcgaatgg gtgaccatgg gcatcccgtc gcagggctgg tatggcatcc ccaaggaggt
    41401 gatgttcggc tttcccgtca cctgcgagaa cggcgaatac aaggtggtcg aagggctgcc
    41461 gatcgatgcc ttcagccagc agtgcatcga caagacgctg gccgagctcg aaggcgagaa
    41521 ggacggcgtc aagcacctgc tgtgaggatg agggctgctg tcgccgaacc gatggggttt
    41581 cgttgtgaaa gaccttggag gccatgtctg gcaagcgcca gcagctcttg ttttgatagt
    41641 ttgccgatgc cgttgcggcg ccgcgctcgc gccgcttgac accaccgcct gatccatgac
    41701 tgccgtcgcg cgccatcccc gacaggtgct gctgggtgcc caggccggtg ccgtggtgct
    41761 gccggtgtgt gaccactaca gcggcgtcga agcgcgcatg cgcaagagcc tgcagctgca
    41821 ggccgagatg acgcaggagt tcggcacctg cgtgttcgac gtcacgctgg actgcgagga
    41881 cggcgccccg gtgggtggcg aagccgagca cgcccagtgc gtgaccgaac tggcactggc
    41941 cgccgcgccc gacgcacgcg tgggcgtgcg cgtgcatccg gtcgatcacc cggcgttcga
    42001 agccgacatg gcgcagatcg cgggcaaggc cgggcaccgg ctggcctaca tcatgatccc
    42061 caaggtggag tcggtggagg atgtggtgca cgccgagcgc gcgctgcggg ccagctttgc
    42121 gccgggcctg ccgttgcagg tgctgatcga gtcgccggcg gcggtgcacc gtgccttcga
    42181 catcgccgcg cacccgggcg tgcagtcgct cagcttcggt ctgatggatt tcgtctcggc
    42241 gcacgccggc gccatcccgg ccgaaggcat gggcatcgag gggcagttca cgcacccgct
    42301 ggtgctgcgc gccaagctcg aaatcgcatc ggcggcccac gcgttcggca aggtgccctc
    42361 gcactgtgtg gtgaccgagt tcagcgacgt gcaggccatg cgcatggccg cgcggcgcgc
    42421 ggcgcgcgag ttcggctacg cgcgcatgtg gagcatccac cccagtcaga tccggcccat
    42481 tctggaggcc tttgcgcccg acgaccggca gatcgacctt gccagcgcca tcctgctggc
    42541 cgcgcagcag gccgactggg cacccatcag ccaccagggt gccttgcacg atcgcgccag
    42601 ctaccgccat ttctggcagg tgctggagcg tgctcacgcc accgggcgcc acttgcccgg
    42661 cgatgtggcc cactggttct gaccccatgc catgtcatga tgagtggttg ttgaaacatt
    42721 cctgaaagcg agttccacat gaagcctttt gccatgacca gcctggccat cgttcttggc
    42781 ctttccatgg gtgcggtcca ggttgcgtcg gcggccgaca aaccggcgcc ggccaagaag
    42841 gtcacgaccc aggccaagaa agcgccgacc aagaaggtgc cggtcaagca ggcagccacc
    42901 agcaagggtg tcccggccaa gacggccaag gcggtcgagg ccaacacccc cgtgcagacg
    42961 ctgagccagc gcctgaccga caccgaactg gagatcgcca aaaccataca gaccggtcgc
    43021 atccagtgcg agcttggtgc cgatgtgacg gtgtccgccg acgagaagaa ccccggtttc
    43081 ttccacgtgg tggcgggcaa gcagcgctac tacatgcacc cggtggaaag ccgcaccggc
    43141 gccgttcgca tggaagacaa ccgcgccggc gccatgtggc tgcagctggg caacaagtcc
    43201 atgctgatga accagaagct gggccagcgc gtcgccgacg agtgcgtgac gccggcgcag
    43261 cgcgagtttg cgttgcagat gaagtccagc aatcaacccg gtctgctgga cggacagacc
    43321 aaatagattt tctgccggcg gctgccggtt tggcctcgtg cgcaggtgcg gggcgagccg
    43381 gctcgcgcgc gggtacctca cgcgtcccat cgatatcacc cagtcaggag aacacagcat
    43441 gctgcaagcc taccgagccc acgccgccga gcgcgccaaa ctgggcatcc cgcccctggc
    43501 gcttgatgcc aagcaggtcg ccgagctgat cgagctcatc aagaacccgc cggccggcga
    43561 agagcaattc ctgatggatc tgctcacgca ccgcgtgccc ccgggcgtgg acgacgccgc
    43621 caaggtcaag gccagctttc tggccgccgt cgcgcatggc gacatccagg tgggcctgat
    43681 ctccaaggcc aaggccaccg agctgctggg caccatggtg ggcggctaca acgtgcaccc
    43741 gctgatcgag ctgctcgacg acgccgaagt ggcccaggtg gccgcggacg cgctcaagaa
    43801 gacgctgctc atgttcgact acttcaacga cgtggccacc aaggccaagg ccggcaacgc
    43861 gaaggccaag gaagtcatgc agagctgggc cgacgccgag tggttcacca gccgtcccaa
    43921 ggtggccgag aagatcaccg tcaccgtgtt caaggtgccc ggcgagacca acaccgacga
    43981 cctctcgccc gcgcccgacg ccacgacgcg ccccgacatc cccatgcact acctggcgat
    44041 gctcaagaac acgcgccccg acgcggcctt caagcccgag caggacggcg tgcgcggccc
    44101 catccagttc atcgaggacc tgaagaagaa gggccatatc gtcgcctacg tgggtgacgt
    44161 ggtgggtacg ggctcgagcc gcaagtcggc caccaacagc gtgatctggg ccacgggcga
    44221 ggacatcccc ttcgtgccca acaagcgctt cggcggcgtg acgctgggcg gcaagatcgc
    44281 tcctatcttc ttcaacacgc aagaggattc tggctcgctg cccatcgagg tcgacgtctc
    44341 caagatggaa atgggcgacg tgctcgacat cttcccctac gccggcaaga tcgagaaggc
    44401 cggtgagaag atcgccgact tcaagctcaa gagcgacgtg ctgctcgacg aggtgcaggc
    44461 cggcggccgc atcaacctga tcatcggccg cgcgctgacg ggcaaggccc gcgagttcct
    44521 gggccttccc gcctcgaccg ccttccgcct gccccaggcg cccgccgaat ccaaggccgg
    44581 cttcaccctg gcgcagaaga tggtcggccg cgcctgcggc ctgcccgaag gccagggcat
    44641 ccgccccggc acctactgcg agcccaagat gaccaccgtg ggcagccagg acacgaccgg
    44701 ccccatgacg cgcgacgagc tcaaggacct ggcctgcctg ggcttctcgg ccgacatggt
    44761 gatgcagtcc ttctgccaca ccgcggccta ccccaagccc gtggacgtca agacccaccg
    44821 cacgctgccc gagttcatca gcagccgcgg cggcgtggcc ctgcgccccg gcgacggcgt
    44881 gatccactcg tggctcaacc gcctgctgct gcccgacacc gtgggcacgg gcggtgactc
    44941 gcacacgcgc ttccccatcg gcatcagctt ccccgcgggc tcgggcctgg tggccttcgg
    45001 cgccgccacg ggcgtgatgc cgctcgatat gcccgagtcg gtgctggtgc gtttcaaggg
    45061 ccagctgcag cccggcgtga cgctgcgtga tctggtgcat gccatcccgc tgtacgccat
    45121 caaggcaggc ctgctgacgg tcgccaaggc cggcaagaag aacatcttct cgggccgcat
    45181 cctcgagatc gaaggtctgc cggacctgaa ggtggagcag gcgttcgagc tgtcggatgc
    45241 gtctgcagag cgttccgccg ccggctgcac gatcaagctc aaccccgagc cgatcaagga
    45301 gtacctgcgg tccaacatcg ttctgatgaa gaacatgatc gccgacggct accaggacgc
    45361 caagaccctg gcgcgccgca tcgagaaggt cgaggcctgg ctggccaacc cgcagctgct
    45421 cgaggccgac aaggacgccg agtacgccgc cgtgatcgag atcgacatga acgagatcac
    45481 cgagcccatc gtctgctgcc cgaacgaccc ggacgacgcc aagttcctgt ccgaagtggc
    45541 cggcaccaag atcgatgagg ccttcatcgg ctcgtgcatg accaacatcg gccatttccg
    45601 cgccgcggcc aagctgctgg gcggccagcg cgacatcccc gtcaagctgt gggtggcgcc
    45661 gccgaccaag atggaccaga acgagctcat caaggaaggc cactacgccg cctttggcac
    45721 cgcgggtgcg cgcaccgaga tgcccggctg ctcgctgtgc atgggcaacc aggcgcaggt
    45781 gcacgagggt gcgacggtga tctccacctc cacgcgcaat ttccccaacc gcctgggcaa
    45841 gaacaccaac gtgttcctgg gctcggccga gctgtcggcc atcgcctcgc gcatgggcaa
    45901 gctgccgacc aaggaggaat acctcaagga aatgggcgtg atcgaccagg acaaggccag
    45961 cgtgtaccgc tacatgaact tcgaccagat cgaggagtac gccgaggtcg ccaagggcgt
    46021 ggcggcctga acgaccgcgg gcacggtcgg caagccgcgc agccgtcagg cgcgcggctt
    46081 gttcaatgtc caatgatcct ggaaggctgt cggacttgga aatcgtcggc tgcaaatcga
    46141 ccgcagcggc ccatttttca ccggcttttt gcccaatagc tcggctcttg ggctgcaaat
    46201 ccggcaaaaa ctggtctcgc tggggccgat tctggctacg tcgctcatcg agaacgcttt
    46261 cggcgcttca agcccgacag cctcccaggt tcaacgtttc agtggactag gatgattgca
    46321 agccaaactg gcactttgcg cttgctgggc aagcgctggc agctattgaa agcgtagcac
    46381 tactcgcgcg tgatcaccgc gcaggcgatg cgcgggccgg agttgccggc cggctgggtg
    46441 ctgaagtcgt cgcgatcgcg gtgcaccacc agcgcgcggc cgacgacgtt gttggccgcc
    46501 ccgtcggtca gcgagatggt gtgcacgtcc acgctgaagc gcgccacgcc ggcagcgtcg
    46561 gccaccaggc tgggcagttc gcccgcgtgg gtgccggcgc cgccgaactt gccgtgcatg
    46621 ccgccagtgg ggttgaagtg gccgcccgag gcgttgccgt tgttgccgca gtcgcccttt
    46681 tcgtggatgt ggaagccgtg ctcgctgccc ggcaccaagc cacgaacctc gccggccacg
    46741 cgcacgccgt gcgccagcgc ggtgaaggtt gcgctgccgg aggtcgggtt gggggtgatg
    46801 gcggcggtgg gcgccaggct ggcgcgcgcc gtggcggtgc ccttgttcat gctgccgcag
    46861 gccgtgagtg cggcgccggc ggcgatgacg gccgcggccg tggtgaagct gttcaggcgg
    46921 atcatttcgg tgctctccgg tgggtgaggg aaatggctgg caggccgtcg ggctggcttg
    46981 cggatgctga ccgggcatca tacccaggct cgccgccgcg cgcaggtccg cggatgccgt
    47041 ttcggtggcc aagcggaagc ggggcgggtg cagcacccgc ctcccgatcg ctattgaaag
    47101 aatagctgcc agcgcttgac tggaaggcgc tggaagtcga tttggccaaa aaccggcggg
    47161 cgctcagagc acctcgctgg cgaagtcggc caggcgcgag cgctcgccgc gcgccagggt
    47221 gacgtggccg ccgtgcggcc agcccttgaa gcggtcgacg gcgtaggtca ggccgctgga
    47281 gccctcggtc aggtaaggcg tgtcgatctg cgccaggttg cccatgcaga tgatcttggt
    47341 gccggggccg gcgcgggtga tcagggtctt catctgcttg ggcgtcaggt tctgcgcctc
    47401 gtcgatgatg atccacttgt tcatgaaggt gcgcccgcgc atgaagttca tgctcttgat
    47461 cttgacgcgg ctgcggatca gttcgttggt ggcggcgcgt ccccattcgc ccgcgccgct
    47521 gccgtcgccc ttggacagga actccaggtt gtcgtccagc gcgcccatcc aggggcccat
    47581 tttttcctct tcggtgccgg gcagaaagcc gatgtcctca cccacgctga cggtggcgcg
    47641 ggtcatgatg atctcggtgt agcgccggtc gtccagcacc tgcgtcaggc ccgaggccag
    47701 cgccagcagc gtcttgccgg tgccggccgt gccggccagg gtgacgaagt cgatctccgg
    47761 gtccatcagc aggttgaggg cgaagttctg ctcgcggttg cgcgccgtca cgccccagac
    47821 ggcgttcttc aggtgggtgt agtccttgag tgtgcgcagc accgcggtct tgccctggat
    47881 ctcggtgacg cgggcgtaca ggctgggctc gcccggggcc tcgaagaaga cgaactgatt
    47941 gatctgcagg ctgggcacca ccggcccgct gatgcggtag aaggtggcgc tgcccgactg
    48001 ccagctctcg accgtcttgc tctggcgcgt ccagaagtcg gtgggcagct gcagcacgcc
    48061 ggcgtacagc aggtcgccgt cgtccagcgt cttgtcgttc tcgtagtcct cggcgtgcag
    48121 ccccagcgcg cgcgccttga cgcgcatgtt gatgtccttg gacaccagca ccacttcacg
    48181 cggggcatgc tgtgtctcca gcgctgccac cacgcccagg atctggttgt cggccttgcc
    48241 ctggggcagg gcggcgggca tggtggcgtc cagcggcgtg gtctgaaaga acaggtggcc
    48301 gctggcttcg acatggccgg tggcatcgag cttcagccct tgcgccatgt cggcgccccg
    48361 gacgccggcc agcgcgtcca gcaggcggct ggtctggcgc ccgttgcgcg ccacttccga
    48421 catgcccttc ttgtgcccat cgagctcctc cagcacgatc atcggcagga agatgtcgtg
    48481 ctccttgaag cgaaacagcg ccatcgggtc gtgcagcagc acattggtgt cgagcacgaa
    48541 caggcgcgcc ggcccctggg tgcgggcctt gcgtgcgcct ggcgtcgatt tggggcgccg
    48601 cgccggcggc gccttgggcg tcgagctgac gggtgccggg gccggtgcct gcagcagggc
    48661 cgccggggca ggggagcccg atggtggcgc cgtccgggcc gagggcgcag accgtgcggg
    48721 cgacgacggc tgggacggcg gggtggccgc gctggtcttg gcacggcgtg tcgccaggtc
    48781 gatcacgtcg ggagacaggc gggcgccgcg ttgggcagga gggggaggca ggggcatgct
    48841 tgtcggctca gcgagcaggg gcggtgccgg ccagcaccgg tgggctggat ggccggcggg
    48901 ccaaagaaaa agccgcctgg gctgccccgg cggctttcag cgagaggaac ggtcggtgcg
    48961 tgaacaacca cattgcggcc cattatgcat gagccggttc ggtggccggc aggcgcgtca
    49021 ggcggccttc ttgaggactt tgaccgcctt gagaacgtct tccacgtggc ccggcacctt
    49081 aaggccgcgc cattcctgca ccacgatacc ttcggcgttg atcaggaagg tgctgcgctc
    49141 gatgcccttg accttcttgc cgtacatgat cttgttcttg accacgccga acatgtggca
    49201 catcttttct tcggtatcgg cgatcagctc gaacggcagt tccagcttgg tcttgaagct
    49261 gtcgtgcgac gccatgttgt cgcggctgac gccgaagacg atggcgccgg ccttgacgaa
    49321 gtccttgtac ttgtcgcgaa actgcatggc ctcggtggtg cagccgggcg tgttgtcctt
    49381 ggggtagaag tacagaacca ccgcctgtcc gaggtgggag ctgttgctga ccttgacgcc
    49441 gccggttgcc atcgcttcga attcaggaag gggtttgttg acaacgatcg ccatatgaac
    49501 ttcaatctcc ggacggtgtt atgggtgacg cggaccacgc agcgcccatg cacagactgc
    49561 tgctgcctcg actgcgcggc gtcgggtgcg gggcctcggt ggcaagcccg tcattctaac
    49621 cgatcggtcc cgctcgtgcc gctcgtgccg gcttccacca gcagtgccgc caccacgcgg
    49681 cggccttcgc cggccagaat gttgtaggtg cggcaggcgg cgccggtgtc catggtttcc
    49741 accccgatgc ccgcttcgat caggccccgc acccaggccg ggcgcggaaa gcgcaggcgg
    49801 gcaccactgc cgaacagcac cagttgcggc tgcagcgcga ccaggagggt gaaatgcgcc
    49861 tccgtcagtt cgtcaaaacg gctgcagttc cagagcgcgt gttcgccatc gctgcgcagc
    49921 accaggctgt gttccagacg ctggccgccg atggcgatcc agccggggcc atagctggtg
    49981 atggcggagc cgaactggtc gggctggaat ttcatggcgg gcggcgtggg cggtggagcg
    50041 aatgtgttcc aattctaggt ttcccccggg atgactgccg cgcgagaggg tttcatgaag
    50101 acgatccaca agtcgaccaa gctggccaac gtgctgtacg acgtgcgcgg gccgatcgtg
    50161 gacgccgccc gccagatgga ggacgagggg cagaagatca tcaagctgaa catcggcaac
    50221 ctggcgccgt tcggcttcga gccgcccgaa gagatcgtgc gcgacatgat ccgcaatctg
    50281 ccggactcgg ccggttactc cgacagcaag ggcatttttt ccgcgcgcaa ggcggtgatg
    50341 cactacacgc aggagcaggg catcgaaggc gtcaccatcg acgacatcta cctgggcaac
    50401 ggcgccagcg acctgatcgg catggccacc aacgccctgc tgtccgaggg cgatgaaatg
    50461 ctggtgccgg cgcccgacta cccgctgtgg acggcggtgg ccagcctctc gggcggcaag
    50521 ccggtgcact accgctgcga cgaggacaac ggctggatgc cgaacctggc cgacatccgc
    50581 gccaggatca cgccggccac ccgcgccatc gtcgtcatca accccaacaa cccgaccggg
    50641 gcgctgtatt ccgacgagct gctgcgcggc atcgtgcaga tcgcgcgcga gcacggcctg
    50701 gtcatcatgg ccgacgaggt gtacga
//
LOCUS       DAOOOG010000004        43606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_624_length_43606_cov_28.319947, whole genome shotgun sequence.
ACCESSION   DAOOOG010000004 DAOOOG010000000
VERSION     DAOOOG010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 43606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43606
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_624_length_43606_cov_28.319947"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PK242_00740"
     CDS             <1..200
                     /locus_tag="PK242_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOZ92584.1"
                     /translation="PQSAILGVHATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREA
                     VLGLVAMKEALEDPARLLFDI"
     gene            271..1701
                     /gene="lpdA"
                     /locus_tag="PK242_00745"
     CDS             271..1701
                     /gene="lpdA"
                     /locus_tag="PK242_00745"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOZ92585.1"
                     /translation="MSNQQFDVIVIGAGPGGYIAAIRAAQLGQKVACIDEWKNDQGGP
                     APGGTCTNVGCIPSKALLQSSEHFEHASKHFGEHGIAVSGLKMDVAQMVARKAAVVKQ
                     NNDGILYLFKKNKVSFFHGRGSFVRAVDGGYEIAVKGAKDETLVGRQIIVATGSSARA
                     LPGVPFDEEQVLSNDGALRIAAVPKRLGLIGSGVIGLEMGSVWRRVGAEVTVLEALPT
                     FLGAVDEQIAKEARKAFDKQGLKIELGVKVGEVKVGKKGVSVAWTDAKGAAQELVVDK
                     LIVSIGRVPNTTGLNAEAVGLRLDERGAVVVDDDCRTNLPGVWAVGDVVRGPMLAHKA
                     EEEGVAVAERIAGQHGHVDFNLIPWVIYTSPEIAWVGKTEQQLKAEGAKYKAGSFPFM
                     ANGRARALGDTTGLVKFLADAATDEILGVHIVGPMASELIAEACVAMAFRASSEDIAR
                     ICHAHPSLGESMKEAALAVDKRTLNF"
     gene            1775..2875
                     /gene="zapE"
                     /locus_tag="PK242_00750"
     CDS             1775..2875
                     /gene="zapE"
                     /locus_tag="PK242_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HOZ92586.1"
                     /translation="MSDVRQAYELELKARGFQSDPAQLRAVQALQRCADDWAAYKSSR
                     SNRLKKLFNRPEIPRGVYMYGGVGRGKSFLMDCFFNAVPIRRKTRLHFHEFMREVHRE
                     LAALQGTVNPLDELGRRMAKRFKLICFDEFHVADITDAMILHRLLVALFDNGVGFVTT
                     SNFKPDDLYPGGLHRDRILPAIELLNQRMEVVNVDNGTDYRHRTLQQVQLYHTPLGAA
                     ADAAMQAAFDQLASRADEDPVLHIEAREILARRRAGGVVWFDFRALCGGPRSQNDYLE
                     IASQFHTVLLSGVPHMPVRMASEARRFTWLVDVLYDRRVKFILSAEVPPEQLYTEGPL
                     AHEFPRTVSRLNEMQSAEYLALEHRTVDTQLT"
     gene            2872..3267
                     /locus_tag="PK242_00755"
     CDS             2872..3267
                     /locus_tag="PK242_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92587.1"
                     /translation="MSRLPISLLLCTLLAACASGSLHDQHLARRAADAADAQALAATA
                     PPAGQPLAPWLQAQRARIVSERGAATRHFEEAEKACWQRFAVNDCLQQARVQRRTALD
                     RLRQQELAINDLERQRQADARRLEPRNGS"
     gene            complement(3805..4605)
                     /locus_tag="PK242_00760"
     CDS             complement(3805..4605)
                     /locus_tag="PK242_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOZ92588.1"
                     /translation="MEKGYRLSAATGLHKGDRDYQQDQVALFAHARANGCVMGVVADG
                     MGGRTGGRKAADQVMLTARQLFERYAPDSEDATAVLRQIGEEAHLIIKLTAIAAEQEP
                     HSTMAAFIINVGGECHWAHTGDSRLYHFRGARLVRRTKDQSYVQALVDQGELTEDEAA
                     VHPKSNVLLGCLGTEEAPKFDVHYIPQLQIGDALLACTDGLWHYFTPEELGSVLHSLP
                     PREASEFLIDKARTRAMGGGDNLALAIVKVEPLQTNQPVVGSAFSPLR"
     gene            4729..4932
                     /locus_tag="PK242_00765"
     CDS             4729..4932
                     /locus_tag="PK242_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HOZ92589.1"
                     /translation="MTANLHSPERRLIELRMEHADLDASIDRLAGQAPLDELMLQRLK
                     KRRLALRDAIARLQAQLEPDEPA"
     gene            4919..7000
                     /locus_tag="PK242_00770"
     CDS             4919..7000
                     /locus_tag="PK242_00770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOZ92590.1"
                     /translation="MNRPERVSPVATPGAPQGPEPGALAQAVGAAFDDHGPLSQAAEG
                     FRLREGQTRLALALARVIEQGGVLVAEAATGVGKTFAYLVPALLSGERVLLSTATKTL
                     QDQLFGRDLPRLARALGLPVSMALLKGRASYLCLHRLEMAHQTGVSSAPGVRQQLSKI
                     ERWSKITRSGDLAELPDLDERSPVIPLVTSTRENCLGADCPQFRACHVQLARRQAMAA
                     DVVVINHHLFFADLAIRESGMAELLPSVRVAVFDEAHQLNETGIQFLGQQLASGQLLD
                     FARDLLAAGLRQARGLCDWQALASAVEVATRELRLAAGAGRAAGRLRWLEAVPEGVDA
                     AAWPPALRQLHQALADAAAALATVSELAPDFVRLHQRALLLAQRAERFGQPCEPALVR
                     WVDVGASLRLVESPLDIADTVRTRLLAGPQDDGRARAWAFVSATLGDDERLSWFTEPC
                     GLQDAEVLRIGSPFDYARQAALYVPTHIARPADPAHSADVARLAGDAAARLGGRTMVL
                     TTTLRALRRIGELLGERFGAGADIEVLMQGSLPKRVLVERFRAGGSAGRPGCVLVASA
                     SFWEGVDLPGQALQLVVIDKLPFPPPGDPLVEARCRRLQAQGRSAFADHSVPEAAVAL
                     KQGAGRLIRRESDRGVLVVCDTRLVQMGYGRRLLAALPPMRRLPSPQAFEAALDELVA
                     EDATKTIAAGA"
     gene            complement(7062..7865)
                     /locus_tag="PK242_00775"
     CDS             complement(7062..7865)
                     /locus_tag="PK242_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOZ92591.1"
                     /translation="MQGARLSVAGAVAAALAVLLAACSSKPIDRTAGWSPNRIHAEAK
                     DELNAGAYDKAIPLYETLEGRAAGTTLAQQAQLDKAYALFKDGQKAEAIATLDRFMRL
                     HPASPAIDYALFLKGVINFNDELGMLSFLARQDLSERDQKAAKEAFESFNELVTRFPD
                     SRYAPDARARMRYTINALAQYEVHVARYYFTRGAYVAAINRAQQALADYRDAPSLEEA
                     LYILTQSYDRLGMTELRDDARRVLDRNYPNSTYLTHGLRGADKPWWQLW"
     gene            7964..8947
                     /locus_tag="PK242_00780"
     CDS             7964..8947
                     /locus_tag="PK242_00780"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOZ92592.1"
                     /translation="MEDAGAAAELRQLALPAELHGQRLDRALAALVPEFSRNYLRQLI
                     EAGVVHMQGQPVVKPAQKVRAGDHLAIELRPTPMSQAFVPQAMALDVVYEDQHLAVID
                     KPAGLVVHPAPGHWSGTLLNGLLARDAAAAHLPRAGIVHRLDKDTSGLLVVARSRAAM
                     DALVALIAARQVRREYLALAHRPWSGAPAREIDAAIGRDPRQRLRMAVLDPARHPARP
                     AQTRFELLQNAPQGCLLRCLLRTGRTHQIRVHLAHLGHPLVADALYGGAPAAGLTRQA
                     LHARRLAFRHPLTGQTLDFVAPLPTDLTHALSAWGLTMPADDMDPAASPAR"
     gene            9049..9852
                     /gene="scpB"
                     /locus_tag="PK242_00785"
     CDS             9049..9852
                     /gene="scpB"
                     /locus_tag="PK242_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOZ92593.1"
                     /translation="MNSTDAKRILETALICAPQPMPLRELRVLFDDELGADTLKNLLL
                     ELQDDWAQRGVELVQVASGWRFQSRPEMREYLDRLHPEKPPRYTRATLETLAIIAYRQ
                     PVTRGDIEDIRGVTVNTQLIKQLEDRGWVEVIGHRETVGRPGLYATTRQFLDDLGLES
                     LDQLPMLDTPSEQAASMFDALDQAAEDAPAAAATDAEPAATPDVEAGEPAAAPAAKAE
                     PESPSAPDADDSAAPAATPASAPEPEPEPVDDSPPSRPDTAEHEAPHVP"
     gene            9842..11203
                     /locus_tag="PK242_00790"
     CDS             9842..11203
                     /locus_tag="PK242_00790"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOZ92594.1"
                     /translation="MCPDEPTAPAADDQASATPAPARRSRTKPTSEPAQTAPNAIDMV
                     AIEADAEPTGAPTAADEAPVVIDDVLSGRFDAEADGGASDVPPKRVLLPQPEQPKLHK
                     VLAQAGLGSRLEMEQLILQGRISVNNQPAHIGQRIQFGDAVKINGKPIKLRIAPPPAR
                     VLAYHKPVGEVVTHDDPQNRPTVFRKLPRLPQGKWQSVGRLDLNTEGLLLLTNSGELA
                     NRLMHPRFGLEREYAVRVLGALSDEEKQQLLDGVPLDDGMAQFSSIQEEGQGEGANQW
                     YRVTISEGRNREVRRMIESVGHAVSRLIRIRYGAMMLPRGLRRGQWMELGERDIRALG
                     AASGTPEHVLRGGREPGPRPSEPPRRGRGDRGRGSGPRPNMAGGGRPPANTGSGKPRR
                     RDGGPAGGAGAPQPDPMKTSFGYIGADSLSRQRQSAGRGAPGARGGGGRARRGPAPGG
                     RRG"
     gene            11326..11751
                     /gene="ndk"
                     /locus_tag="PK242_00795"
     CDS             11326..11751
                     /gene="ndk"
                     /locus_tag="PK242_00795"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOZ92595.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIYARFEAAGLKIVAARMQHLSR
                     REAEQFYAVHKERPFFKDLVEFMISGPVMIQVLEGEGAIAKNRDLMGATDPKKADKGT
                     IRADFADSIDANAVHGSDAPETAAVEVAFFFSGMGVYSR"
     gene            11779..12945
                     /gene="rlmN"
                     /locus_tag="PK242_00800"
     CDS             11779..12945
                     /gene="rlmN"
                     /locus_tag="PK242_00800"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOZ92596.1"
                     /translation="MTTPVNLLDFDLEGLAAWCDTLGEKRYRATQLFRWIHQRGVAEF
                     GQMSDLARSLQGKLHGRALVAAPAVIRQHASKDGTIKWLFDVGGGNAVEAVYIPEDDR
                     GTLCVSSQAGCAVACRFCSTGHQGFSRNLSTAEIVGQLWFAEHFLRRHLKTDERVISN
                     VVMMGMGEPLQNYDRLLPALRTMLHDHGYGLSRRRVTVSTSGVVPMIERLARDCPVAL
                     AVSLHAPTDALRDHLVPLNRKYPIDELLRTCTAYLAHAPRDFITFEYCMLDGVNDSAE
                     HAQALVELMRRHAASGLRGKFNLIPFNPFPQSGLTRSPMARVQAFGKLLNDAGIVTTI
                     RKTRGDDIDAACGQLAGDVQDRTRVGVRIARQRTVALHQLAPAGAAPAPRKVSS"
     gene            12942..13772
                     /gene="pilW"
                     /locus_tag="PK242_00805"
     CDS             12942..13772
                     /gene="pilW"
                     /locus_tag="PK242_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HOZ92597.1"
                     /translation="MSLHACAPSAASRRRLLALASFGLAAWLLAACAAPPGLGDNPQE
                     LPTASDETDARRRARTRLALATGYYENGQHTVALDEQKKALQADPNFGDAYNLGGLIY
                     MALGDQALAQSHFQRAIALNPRDGDALHNLGWLQCQQGQLAAANQSFERALAVPTYPG
                     RARTLMAQGVCEARAGNLARAEAALMRSYELDAGNPVTAFNLSQLLYQRGDYTRAQFY
                     VRRLNNSELANAESLWLGIRVERRLNNRQAMEQLASQLRRRFPQSRELLAYERGAFDD
                     "
     gene            13765..14646
                     /locus_tag="PK242_00810"
     CDS             13765..14646
                     /locus_tag="PK242_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ92598.1"
                     /translation="MTEAHTGPASLHDTPAHGAGPRSAGQMLRALRESAGVDPALLAS
                     AMKVSPQKLDALENDRLDELPDVTFARALASAICRAFGTDPAPVLERMPVSAPGLRPV
                     AQAPINEPFRRSSDGPATVLAGGLSKPLAIGVIALLLGAAALWLLPTLPIQLNAPAPA
                     SEPAATDGVVRESVMPPAATSAEPPAPPAPAELSASAAAAEQAPPPAAAAPAAASTDL
                     LYFSAQAETWVTVRDASGKNLINRALNAGDSVGVSGEPPLAVTIGRKDAVEVRVRGEP
                     FDHRSISKTPVSRFEVR"
     gene            14649..15917
                     /gene="ispG"
                     /locus_tag="PK242_00815"
                     /gene_synonym="gcpE"
     CDS             14649..15917
                     /gene="ispG"
                     /locus_tag="PK242_00815"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOZ92599.1"
                     /translation="MPSPNCTPIEVARPAPRRTRQARVVWGRRVVTVGGDAPVRVQSM
                     TNTDTEDAIATAIQVKELALAGSELVRITVNTPEAAAQVPYIREQLDRMGIDVPLIGD
                     FHYNGHRLLTEFPDCAQALSKYRINPGNVGKGAKRDTQFGQMIEAALKWDKPVRIGVN
                     WGSLDQDLLAGLMDENSRRAQPWDARQVMYEALISSALGSASQAVELGMDPAQVILSC
                     KVSGVQDLIAVYRELASRGDHPLHLGLTEAGMATRGTVASSVALAILLQEGIGDTIRV
                     SLTPQPGEARTQEVVVASEILQALGLRAFVPSVTACPGCGRTTSTVFQELAKQIDDHL
                     RAQMPLWRSQYPGVEKLKVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVYIDGEK
                     AMTLRGEHIAAEFQQIVARYIEQRFGRRQP"
     gene            15994..17301
                     /gene="hisS"
                     /locus_tag="PK242_00820"
     CDS             15994..17301
                     /gene="hisS"
                     /locus_tag="PK242_00820"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOZ92600.1"
                     /translation="MSGSKQITAIKGMNDILPPASARWEWLEDVVRRVMAQHAYRNVR
                     TPIMELTQLYTRGLGEVTDIVEKEMYAFEDRADQHGRADHLALRPEGTAGLVRAVAEH
                     NLLYDGGKRLFYMGPMFRRERPQRGRYRQFHQIGAEALGFGGPEVDAELILLAVALWR
                     ELGLTGWRLELNSLGQPDERREHRAALVAYLQAHHDQLDEDARRRLHSNPLRILDTKN
                     PAMQALVEGAPKLLDYLGEASRRHFAAVQAILAAAGVSWHINPRLVRGLDYYNLTVFE
                     FVTDRLGAQGTICGGGRYDYLIEQIGGKPAPAVGWALGVERVLELLQEEGVHTLEPVP
                     DVYAIIPDAAALPQAMRCLQQLRALGVAVQMHAGSGEGMGSMKSQFKRADASGARHAL
                     IFGADELAAGEVTVKALRDGAGAQLRQPLAQPDAWADILKSTA"
     gene            17319..17996
                     /locus_tag="PK242_00825"
     CDS             17319..17996
                     /locus_tag="PK242_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ92601.1"
                     /translation="MATHLDLEEQEQLDALKHFWNRWGNLITWVLIAVLGAYAAWNGW
                     QYWERRQAGLAAVLFDEVERAAQAGDAARVERALADIKDKFGGTTYAAHAGLRGARFF
                     DEQGKTAETKAALAWVAEKAPNDGLRAVARLRLASVQLGEKAYDEALRTLEAGFPPSF
                     VALAADRRGDVLLQQGKRELAAAEFGKAYQGMAADTAGDYRRLVGIKLNALGIDPDAG
                     AKAGAAS"
     gene            17993..19132
                     /gene="bamB"
                     /locus_tag="PK242_00830"
     CDS             17993..19132
                     /gene="bamB"
                     /locus_tag="PK242_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HOZ92602.1"
                     /translation="MTARGTVRRRIACGASLLAALVLTACAGGSPRPQPAELPANIAL
                     IGVRQAWTARLPAIDFPLQVAVQGQQVTLAAADGSVVTLDAASGRELGRASAGAPLTA
                     GVGSDGRVSAVVTRDNRVVALEGGRELWRQKLGTQSYTAPLVAGGRVFVLGADRSLSA
                     FDGRNGARLWHLERPAEPLVLRQAGVLQAVGNTLVAGLAGRLVGVDPDSGSVRWEAPI
                     ASARGTNDVERLIDLVGGVSRHEGVVCARAFQTAVGCVDTASGRLRWSKAASGATGVA
                     GDGQLLFGAEADGRVLAWRADTGDRAWVNESLQWRRLTAPLVLGRSVVVGDGNGLLHF
                     LSRQDGTPLDRLSTDGTGIAATPVAAGNTLVVATRGGTVYGFVPE"
     gene            19268..20614
                     /gene="der"
                     /locus_tag="PK242_00835"
     CDS             19268..20614
                     /gene="der"
                     /locus_tag="PK242_00835"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOZ92603.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTRSRDAIVADFAGLTRDRHYGQ
                     GHLGKREYIVIDTGGFEPRAESGIYKEMAKQTRQAVAEADVVLFLVDARAGLSAQDHD
                     IADYLRRLGKPCLLVANKAEGMREGAQLAEFYELGLGAVIPVSAAHGQGVRSLLEGAL
                     DALDLPDVDEAPPDAADGVIRLAVAGRPNVGKSTLINTWLGEERLVAFDLPGTTRDAI
                     SVPFERAGRQFELIDTAGLRRKGKVFEAIEKFSVVKTLQAIESAHVVLLLLDATQGVT
                     DQDAHIAGFILEAGRAVVLAINKWDAVDDYQRELVRRSVESRLGFLKFAELHFISVRK
                     RQGLGPLWASIARAHQAAMRKLPTPQLTRTLLEAVQFQAPKRAGMYRPKLRYAHQGGM
                     NPPVIVVHGNSLEGVTDTYKRYLEGRFRQVFDLIGTPLRIEMKTTHNPYATRPDQG"
     gene            20761..21006
                     /gene="hfq"
                     /locus_tag="PK242_00840"
     CDS             20761..21006
                     /gene="hfq"
                     /locus_tag="PK242_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOZ92604.1"
                     /translation="MSNKGQLLQDPFLNTLRREHIPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNNVTQMVYKHAISTIVPGRPVNFSATGEVAESD"
     gene            21093..22289
                     /gene="hflX"
                     /locus_tag="PK242_00845"
     CDS             21093..22289
                     /gene="hflX"
                     /locus_tag="PK242_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOZ92605.1"
                     /translation="MPRTATPERAAAILVGVDLGVPHFDGQLQELGLLAETAGLQPVA
                     RLTCKRQAPDAALFVGRGKVEEIRLLAQSEGAREVLFDQALSPVQQRNLERAIGLPVN
                     DRTLVILEIFAQRARSHEGKLQVELARLQYLSTRLVRRWSHLERQQGGIGGRGGPGET
                     QIELDRRMIGEAIKRTKDRLQKVKRQRQTQRRQRERRHTFNISLVGYTNAGKSTLFNA
                     LVKARAYAADQLFATLDTTTRQLYLGQAGRSVSLSDTVGFIRDLPHGLIEAFEATLQE
                     AADADLLLHVVDAANPGHLEQIEEVQRVLREIGAAELPQLLVFNKLDELEPARRPLQL
                     KDSVTLDGRELTRIFVSARSGEGLPLLRAELEAAVQSQALEAGGDGVPAPIGHNDGLH
                     SREVDA"
     gene            22286..23632
                     /gene="hflK"
                     /locus_tag="PK242_00850"
     CDS             22286..23632
                     /gene="hflK"
                     /locus_tag="PK242_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOZ92606.1"
                     /translation="MNDRTSAPRPASWPGRIRGMFNLHDPRWGRDDEPSGQEPGQRPD
                     EPQRPPPRGQGPQAGPPDLDELWRDFNRKLGGLFGGGRGRGRNPYSGGGMPPEMKGRG
                     AGIGIGVIAAVALAIWLASGVFIVREGEQAVVTTFGRFDGIRKAGLNWRMPYPIQRHE
                     TVRVTQIRSVDVGRDSIIRSTGLRESAMLTQDENIVEIKFAVQYRLNDARAYLFESKS
                     PDDAVVQAAESAVREVVGKMTMDTAMAEERDQIAPRVRDLMQAILDRYQVGIEVVAVN
                     MQQGGVRPPEQVQAAFDDVLKAGQERERVKNEAQAYANDVVPRAVGTAARLKQEAEGY
                     KARIVAQAQGDAQRFSSVLSEYQKAPQVTRERMYLDAMQQIYGGVNKIIIDSRAGGNL
                     MYLPLDKLLQGAAGAGAAAGAAPAAAQAPAPATAPAAPAADPRARDNARTRERETR"
     gene            23648..24535
                     /locus_tag="PK242_00855"
     CDS             23648..24535
                     /locus_tag="PK242_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOZ92607.1"
                     /translation="MNRVGFIGTTVLILLALLGSTVFVVDQRQFGVVYQLGQIQRVIT
                     QPGLNFKLPPPMQNVTYIDKRLLTLDSMDTEPVLTAEKQRVVIDWYVRWRITDPQAYI
                     RNVGLDESAGALQLNRVVRNAFQEEINRRTVRELISTKRETLMADVKREVSDAVKGAK
                     PWGIDIIDVRITRADYVESITESVYRRMQAERQRVANELRSTGAAEGEQIRANADRQR
                     EVTVADAYREAEKVRGEGDAQATRIYGDAFGRDPQFAEFYRSLDAYKASIGKKGDVLV
                     IDPASSDFFRNLRGAPARR"
     gene            24553..24738
                     /locus_tag="PK242_00860"
     CDS             24553..24738
                     /locus_tag="PK242_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HOZ92608.1"
                     /translation="MDGATWLAALGLALVLEGLVPFLAPRAWRQAFSQLLQLRDGQVR
                     FFGLLALAGGLALLWLA"
     gene            24808..25971
                     /locus_tag="PK242_00865"
     CDS             24808..25971
                     /locus_tag="PK242_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOZ92609.1"
                     /translation="MSAWLLPDHIADVLPSEARHIEELRRELLDTARGYGYELVIPPL
                     IEHLESLLTGTGEALGLQTFKLVDQLSGRSLGVRADTTPQVARIDAHLLGRQAVTRLC
                     YCGPVLHTQPERPHATREPLQLGAEIYGHAGREADLEILSLALDCLRAAPVHGLSVDL
                     ADARIVRALLAGVLVDPERLTEVHLALAAKDASELARLTAAFPASARQGLCALVGLYG
                     DAGVLDEAEKALSGAPGVREVLSNLKWLASQLEGVRVSFDLADARGWGYYTGARFAVY
                     AEGAGDALLRGGRYDGVGAVFGHKLDRERPAVGFSLDLKQLVGVAAPRPLRAAIRAPW
                     GDAPGLRKAVAGLRRRGETVVCALPGHADQIDEFHCDRELIEAGGQWVIQALG"
     gene            26081..27460
                     /locus_tag="PK242_00870"
     CDS             26081..27460
                     /locus_tag="PK242_00870"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOZ92610.1"
                     /translation="MKPSTTGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHN
                     AGHTLVINGVKTALHLIPSGIMRPGVTCYIGNGVVLSVAKLLEEIAGLERAGVQVRER
                     LRISEGCPLILPLHAAMDVAREAAREQGGSEKIGTTGRGIGPAYEDKIARRALRAQDL
                     KHPERFAVKLRELLALYNHELLGYLGSARIAFNEALTPYLKDGALQFEPIYDEAMRQA
                     EQVRPMLADVSRELNEAHQQGANLLFEGAQGTLLDVDHGTYPFVTSSNTVAGNAAAGA
                     GVGPGLLHYVLGITKAYCTRVGGGPFPTELDWQTPGTPGWHMSTVGAEKGVTTGRSRR
                     CGWFDAALLKRSAQVNGLSGLCITKLDVLDGLPELQLCTGYELDGETIDILPLGADDI
                     ARCKPIYETMPGWSESTVGATRYEQLPIHARLYLQRIEHVTGVPIHVVSTSPDRDHTI
                     MLRHPFLPT"
     gene            27530..28054
                     /locus_tag="PK242_00875"
     CDS             27530..28054
                     /locus_tag="PK242_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOZ92611.1"
                     /translation="MLTEDGKHLYVSYDEYHALIEKLALKVHQSGWRFDTILCLARGG
                     MRPGDILSRIFDVPLAIMSTSSYRAEAGKVQGHLDIAHYITTPQGQIAGRVLLVDDLA
                     DTGVTLQAVVERLRNDYPPITELRTAVIWTKGSSSFQADYSVEHLPTNPWIHQPFESY
                     DGLTPDKLLAKWKV"
     gene            complement(28218..29156)
                     /locus_tag="PK242_00880"
     CDS             complement(28218..29156)
                     /locus_tag="PK242_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92612.1"
                     /translation="MFDRTRFRTLLGATALTWAAWAQAVPLSVSFDNFTYSGTVTRYA
                     TLADAQNQTNPTGGPYTIQTATNGANSTLPNARDGQISVTSQAPSAYGSDLTYMSTPW
                     YFTLVPASGNGWGNPNNTNDGFIQYYQESSPTVTGGWQPGHTQFRLTVSGGHGDDLDA
                     GRLWPAPDSGAAAISRGAFADFHLDVTANFASPATLNGSTGWYETSAMPSSFSGTLRG
                     IFLNDQPSNPTYNGYYRFNYTVTGPGSWAANNGATWSSGATSYGPSSEWAAPQAAALA
                     APAPVPGPGKIALLGLSLLLGVMAMRRSGMRLGRGF"
     gene            29408..30625
                     /locus_tag="PK242_00885"
     CDS             29408..30625
                     /locus_tag="PK242_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOZ92613.1"
                     /translation="MTDPITQLIHHPYQPPAGFAAPQPAVHKASTVFFPNVAAMRERD
                     WKHKSGYTYGLHGTPTTFLLEERIATLEGGLQCVLVPSGLAAIANVALALLQTGDEVL
                     IPDNAYGPNKALAEGELAAWGITHQYYDPMDLADLAARIGERTRLVWLEAAGSLTLEF
                     PDLIGQVRLCRARGVPCALDNTWGAGLAFNPFDLDGQGLGVDLSAHALTKYPSGGGDV
                     LMGSVTTRDEALHLKLKLTHMRLGLGVAANDAEAVLRALPSVALRYRAQDQAARALAG
                     WCAQQSAFAQVLHPALADAPGHAHWQQVCGAADARGQGAAAGLFSAIVHERYSQAQVD
                     AFCDTLKRFKLGYSWGGPISLVVPYDLAALRARGWPAHLRRGTLVRFSTGLEALVDLQ
                     ADLQQALNRALPS"
     gene            30900..31697
                     /locus_tag="PK242_00890"
     CDS             30900..31697
                     /locus_tag="PK242_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HOZ92614.1"
                     /translation="MVPLGLSDPLRWLGRGWRDFMAAPGIGLFYGVCFWLMAALLALV
                     FRSKPEYVMSIASGCLLVGPFLAMGLYEVSRRREQGVPPELAASLTCWDRHVRSMGML
                     VLVLIVLELLWGRASLVVFAVFFNTGMPSTTGVLQAIFNPDNLEFVLVYLTVGGFFAL
                     LVFSTTVVSIPMILDRDTDAISAAITSIRVVFANPLTLLWWGLLITLLIALSLLSLGL
                     GLLLVGPLLGHASWHAYRGAVQWREPPPRPADACVPAPVPAPAPLPG"
     gene            complement(31601..32779)
                     /gene="mnmH"
                     /locus_tag="PK242_00895"
     CDS             complement(31601..32779)
                     /gene="mnmH"
                     /locus_tag="PK242_00895"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOZ92615.1"
                     /translation="MSVQTIAAADALARLAEFSAIIDARSESEFAHDHLPGAVNWPSL
                     NDQQRARVGTLYKQASPFEARKLGAALVARNIADHIERELAHTSRRWRPLLYCWRGGQ
                     RSGALALVLGQIGFQVTLIEGGYKAFRVALLTDIPAQVQRLRLRVVCGPTGSGKTRLL
                     HALAAAGAQVLDLEGLAQHRASVLGLIPGQPQPSQKHFEMRVWDALRHLEPARPVYVE
                     AESKKVGNVTVPEALIAAMRASDCLRIELADDERVALLLEDYPFFVRDPDFFCQRLDT
                     LVALRGRAVIDDWKARVAAGHTDQVVRELLARHYDPGYASSTQRNFARFGQALAVPLA
                     DRSPASLRAACALILASQAPATPPDGVQPGSGAGAGTGAGTQASAGRGGGSRHCTAPR
                     "
     gene            complement(32776..33408)
                     /locus_tag="PK242_00900"
     CDS             complement(32776..33408)
                     /locus_tag="PK242_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HOZ92616.1"
                     /translation="MNRRDCIRFLLAAAPAGLAAQTAAPPLILVLGDSISAEYGIARG
                     SGWVALLEQRLAREQIAARVLNASVSGDTTAGGRSRLPALLKAHRPAVVVIELGGNDA
                     LRGLSLEMTEANLTAMTQAAQAAGAKVLLAGMQMPPNYGADFGRRFFALYPQVAKAQR
                     AALVPFLLKGVADGDHAQALFQPDRIHPTAAAHPIILDNVWPELRRLLKP"
     gene            33424..34161
                     /locus_tag="PK242_00905"
     CDS             33424..34161
                     /locus_tag="PK242_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ92617.1"
                     /translation="MTDVSHGAPAGARRAPIIAVEHVFKSVTDSSGTLDILRDIHFSL
                     AERETVAIVGASGSGKSTLLSIIAGLDTPTRGTVRIAGDDLFAVSEDARAALRARRIG
                     FVFQSFQLLGNLTALENVMLPLELAGRRDARAAATEMLARVGLAERLRHYPKVLSGGE
                     QQRVALARAFVLHPAVLLADEPTGSLDHATGEKVMALMFELNREQGTTLVLVTHDRGI
                     AARCQRRLTIDAGCIAADEQAESLSIK"
     gene            complement(34203..35684)
                     /locus_tag="PK242_00910"
     CDS             complement(34203..35684)
                     /locus_tag="PK242_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOZ92618.1"
                     /translation="MIRLPTLSPLEHAAFVAAGLLLYVLVTRARQQRRHPYAALAWVL
                     GIAAFPYLGVPLFLLFGTRKTVRPSTPRQPVPAGPWARLAPAWATGLLAALGVPEARP
                     QAALRFHADGAQALAELRALIASAERTLDVCTYVLGDDQVGAAVADELAARARAGVRV
                     RLLVDSVGSLRSRRSHDARLRAAGVRVRRFMPALRNPRRGRTNLRNHRKLVVADGLRL
                     WGGGRNLADEYFIGRAGEAPWIDLSFSAEGALATQAMALFEGDWRIALGLRRASGVPY
                     AERALRHEALLHAYQPADASAAALASAPPAASGAAPSAQVALAHWVPSGPDFHEDVLH
                     ALLVSAAFHAQQRLLLATPYFVPDEGLLEALLLAAKRGVRVTLLLPRRSNHRLADWAR
                     GRAVRALVEHGVEVRLLPTMLHAKAVVVDQALALCGSANLDSRSLFINYEAMAAFYSR
                     AEIDWLAGWITRTAADGEPASARPPGLLRDVLEGVVGALAFQL"
     gene            35928..36662
                     /gene="rlmB"
                     /locus_tag="PK242_00915"
     CDS             35928..36662
                     /gene="rlmB"
                     /locus_tag="PK242_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOZ92619.1"
                     /translation="MSTPKVLFGFHAVGVRLKTAPASVIEVYVDPGRRDARMKQFLAR
                     AAEAGVRVIEADGPRLARLCGGAGHQGVVARVQPIAQTHSLDELLDGVAEPPLLLVLD
                     GVTDPHNLGACLRVADGAGAHAVIAPKDHAAGINATVAKVASGAAETVPYLMVTNLAR
                     TLGELKERGIWVIGTSDDAPQTLYQADLKGPTALVLGAEGPGMRQLTRKSCDLLVRIP
                     MHGAVESLNVSVASAVCLYEARRQRG"
     gene            37115..38185
                     /locus_tag="PK242_00920"
     CDS             37115..38185
                     /locus_tag="PK242_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ92620.1"
                     /translation="MPSSLVARPAEPSRRLLLRLAAAGALGLAGAAAAQRPTPERTSV
                     TLAVGGKTSLPCLPLTIAEQLGYFRDEGLQVELQDHPGGGLAQQALLQGRADVAAGGF
                     EHPVLLRPRGTHCRAFVLLGRAPQLVFGVGTRALPEFRSLAQLRGRRVGVTSADSSSH
                     WFAQRLLARAGLAPTDVEYVGVGTAITPVAALREGRIDALADIDPAISLLEYRADIRV
                     VADTRSLRGTHELYGGPMPGACLYARDAFVLRYPQTVQALTNAVVRALKWLHTAGPSD
                     IVRTVPESYMHGDRSVYLSALDKAREALSPDGMASDDSVATAHRIVAQQLQATDGLRA
                     TAVGASHTNDFARRARQRFLVS"
     gene            complement(38217..39809)
                     /locus_tag="PK242_00925"
     CDS             complement(38217..39809)
                     /locus_tag="PK242_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOZ92621.1"
                     /translation="MKWTLSLTLLAGLWLAGCAAPPGPATPERDVSTATPTRAEPRGA
                     QRMPDALPPVRITELRGNHVAALETPADLWERIRRGYAMPDLQDELVQRHEQWYSTRP
                     DYIERMVERSRLYLFHVVEELELRGMPTELALLPYIESAFNPQAVSSAKAAGMWQFMP
                     GTGRDFQLTQNILRDDRRDVLDSTRAALDYLQKLHDMFGDWHLALAAYNWGQGNVKRA
                     IERNQAAGLGTGYLDLKMPAETRNYVPKLQAVKNIIAQPQRHGAVLPLIENHPFFDTV
                     DIKHDIDVDVAARLAQVRLEDFKALNPSQRKPVIFAAGTPQVLLPWNNAALFQQALAK
                     ADPGTLSSWTAWVAPQNLPSREVASRFGMDESDLREINNLPHGVVIKSGSTVLVRRAN
                     GSATAVASHVVNNAQLAYAPEIVLRRTRVRARKGDTIASVAARYDLPAATVAGWNGTQ
                     AGARLKPGQSVVMFLPVRASSAAARESADSPRSARADRSPGKTKARAAGGAKQAAAPT
                     RAARGGKAAAAAPGKGGRKARR"
     gene            complement(39806..40576)
                     /gene="gloB"
                     /locus_tag="PK242_00930"
     CDS             complement(39806..40576)
                     /gene="gloB"
                     /locus_tag="PK242_00930"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HOZ92622.1"
                     /translation="MDLIALPAFEDNYLWLLHDGQQALVVDPGDAAPVQAALRRLGLR
                     LACILVTHHHGDHTGGVTALREASGATVYGPARERIPEPFTPLAGGDRLRVLGLDFEV
                     LDVPGHTAGHIAFFARDVDGAPLLFCGDTLFSGGCGRIFEGTPAQLLASLDALAALPP
                     TTRVCAAHEYTLGNLRFARTVEPDNTALADHERQCLQRRADGRPTLPSTIGLERAINP
                     FLRSREAAVTRAVRAHSPSANDAVAVFAALRQWKNEFR"
     gene            40664..41455
                     /locus_tag="PK242_00935"
     CDS             40664..41455
                     /locus_tag="PK242_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ92623.1"
                     /translation="MHHWFQTPPGQYLLAWERAQFDAALADVFGYHALQLGLADIDAL
                     AANRMPHRWLALTALPPAADEAAPSAGRWALVSDSTALPFAEASLDLVVLPHTLELSP
                     DPHATLREVQRVLVHEGKVAIAGLNPISLWGLRQWRSHLYRGLGGGQPFLPQAGEFIG
                     HWRLRDWLQLLQFELESVSFGCWRPAVRSPRRLARYAWMDRLGARWWPVFGAAYVMLA
                     VKRTHGARLIGARWKAAPVAATAPATVAHRRADAPTLTETELEPR"
     gene            41442..41879
                     /gene="rnhA"
                     /locus_tag="PK242_00940"
     CDS             41442..41879
                     /gene="rnhA"
                     /locus_tag="PK242_00940"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOZ92624.1"
                     /translation="MNHVEIYTDGACKGNPGPGGWGVLLRSPGHEKELYGGEPQTTNN
                     RMELTAVIEGLRALKRPCEVDLYLDSQYVRQGITEWIAGWKARGWRTASKQPVKNVDL
                     WQQLDALVGGAGHRIRWHWVRGHTGHEGNERADALANRGVPRG"
     gene            42057..43322
                     /locus_tag="PK242_00945"
     CDS             42057..43322
                     /locus_tag="PK242_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ92625.1"
                     /translation="MVAVGALLALSAGAWWWQRAGGSAPAGGPVPAASAARGPGNGAA
                     GGAGGGAPPVVELATARAMVLSDSASAVGSLRARQSVVLRPEVAGRVTQLGFRDGERV
                     RRGQLLVQLDDRLALAQVQQARAELGIAESNHRRNADLAGQGFISARAVDESQAAVAV
                     ARAKLELAQASAARLKILAPFGALAGIGNVHVGDVLQAGADIVHLDDLDAMLLDFRLP
                     ERWQGRVRRGQISHVTLDALPGHAFEAVIQAIDPQIDAQGRSVAVRACIDNRQALLRP
                     GMFARVDTRFGERSGAVMLAEEAVLSQGGNLYVYRLAAGPAAGASAPEGTAEVERVPV
                     TLGARQAGLVEAVSGVAAGERIVVAGQQRLNAARTRVRLTRAADAAAGADGPAPAASA
                     AAAAPAPAAPLQWHRAGSGGNACAAAARG"
     gene            43441..>43606
                     /locus_tag="PK242_00950"
     CDS             43441..>43606
                     /locus_tag="PK242_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ92626.1"
                     /translation="MQLAEVSIRRPVLATVLSLLIVLIGLVSYSRLAVREYPKIDEPV
                     VTVSVRYVGAS"
BASE COUNT         6471 a        15530 c        15426 g         6179 t
ORIGIN      
        1 cgccgcagtc ggccatcctg ggcgtgcacg ccaccaagga ccgcgccgtg gtcgagaacg
       61 ggcaggtcgt ggtgcgcccc atgaactacc tggcgatgag ctacgaccac cgcatcatcg
      121 acggccgcga agccgtgctg ggcctggtgg cgatgaagga agcactggaa gacccggcgc
      181 gcctgctgtt cgacatctga tcggatcacc ccacggccct tggcgggccg tgcccctgtg
      241 gcgggctggg cccggaacgg aacaacgcac atgagcaacc aacaattcga cgtcatcgtc
      301 atcggcgccg gtcccggcgg ctacatcgcc gccatccgcg cggcacagct gggccagaag
      361 gtggcctgca tcgacgagtg gaagaacgac cagggcggtc cggccccggg cggcacctgc
      421 accaacgtgg gctgcatccc gtccaaggcg ctgctgcagt cgtccgagca tttcgagcac
      481 gccagcaagc acttcggtga gcacggcatc gcggtcagcg gcctgaagat ggacgtggcc
      541 cagatggtgg cgcgcaaggc cgccgtggtg aagcagaaca acgacggcat cctgtacctg
      601 ttcaagaaga acaaggtcag cttcttccac ggccgcggat cgttcgtgcg cgccgtggat
      661 ggcggctacg agatcgccgt caagggcgcc aaggatgaga ccctggtcgg caggcagatc
      721 attgtcgcca ccggctccag cgcgcgtgcg ctgcccggcg tgccgttcga cgaggagcag
      781 gtgctcagca acgacggcgc gctgcgcatc gctgccgtgc ccaagcgcct gggtctgatc
      841 ggctcgggcg tgatcggtct cgaaatgggc agtgtgtggc gccgcgtggg cgccgaagtc
      901 acggtgctgg aagccttgcc gaccttcctg ggtgcggtgg acgagcagat cgccaaggaa
      961 gcgcgcaagg ccttcgacaa gcagggcctg aagatcgagc tgggcgtcaa ggtgggcgag
     1021 gtcaaggtcg gcaagaaggg tgtcagcgtc gcctggaccg acgccaaggg cgcagcgcag
     1081 gagctggtgg tcgacaagtt gatcgtctcc atcggccgcg tgcccaacac caccggcttg
     1141 aacgccgagg ccgtgggcct caggctcgat gagcgcggtg cggtggtggt ggacgacgac
     1201 tgccgcacca acctgcccgg cgtctgggcg gtgggcgacg tggtgcgcgg ccccatgctg
     1261 gcgcacaagg ccgaggaaga gggcgtggcg gtggccgaac gcatcgccgg ccagcacggc
     1321 cacgtggact tcaacctgat tccctgggtc atctacacca gccccgagat cgcctgggtc
     1381 ggcaagaccg agcagcagct caaggccgag ggcgcgaagt acaaggccgg cagcttcccc
     1441 ttcatggcca acggccgggc ccgcgcgctg ggcgacacca ccggcctggt caagttcctg
     1501 gccgacgccg ccaccgacga aatcctgggc gtgcacatcg tcggccccat ggccagcgaa
     1561 ctgatcgccg aggcctgcgt ggcgatggcg ttccgcgcca gcagtgaaga catcgctcgc
     1621 atctgccatg cccacccctc gctgggcgag agcatgaagg aagctgccct ggcggtcgac
     1681 aagcgcaccc tcaatttctg atgaaatcgg ccattggcgc ttgtgcagaa agcgcgagca
     1741 gctatttttt tcatagcatc ggtggtctga tcgcatgagc gatgtgcgcc aggcctacga
     1801 gctcgagctg aaggcgcgtg gcttccagag cgacccggcg cagctgcgcg ccgtgcaggc
     1861 gctgcagcgc tgcgccgacg actgggcggc ctacaagtcc agccgttcca accggctcaa
     1921 gaagctcttc aaccgccccg agatcccgcg cggcgtctac atgtacggcg gggtggggcg
     1981 cggcaagagc ttcctgatgg actgcttctt caacgccgtg ccgatccggc gcaagacgcg
     2041 cctgcacttt cacgaattca tgcgtgaggt gcaccgcgag ctggcggcgc tgcagggcac
     2101 ggtcaacccg ctcgacgagc tgggccggcg catggccaag cgcttcaagc tgatctgctt
     2161 cgacgagttt cacgtcgccg acatcaccga cgccatgatc ctgcaccgcc tgctggtggc
     2221 gctgttcgac aacggcgtag gcttcgtcac caccagcaac ttcaagcccg acgacctgta
     2281 cccgggcggc ctgcaccgcg accgcatcct gccggccatc gagctgctga accagcgcat
     2341 ggaggtggtc aacgtcgaca acggcaccga ctaccgccac cgcacgctgc agcaggtgca
     2401 gctctaccac acgccgctgg gcgcggcggc cgacgccgcc atgcaggcgg ccttcgacca
     2461 gctggccagc cgcgccgacg aagacccggt gctgcacatc gaggcgcgcg agatcctggc
     2521 gcgccggcgc gccggtggtg tggtgtggtt cgactttcga gcgctgtgcg gcggcccgcg
     2581 ctcgcagaac gactacctgg aaatcgccag ccagttccac accgtgctgc tgtccggcgt
     2641 gccgcacatg ccggtgcgca tggcgtcgga ggcgcggcgc ttcacctggc tggtggacgt
     2701 gctgtacgac cgccgggtca agttcatcct gtcggccgag gtgccgcccg aacagctcta
     2761 caccgaaggt ccgctggcgc acgagtttcc gcgcaccgtc agccgcctca acgagatgca
     2821 gtcggccgag tacctggcgc tggagcatcg caccgtggac acacaactga catgagccgc
     2881 ctgcccatca gcctgctgct gtgcaccctg ctggccgcct gcgcgtccgg ctcgctgcac
     2941 gatcagcacc tggcccggcg cgccgccgac gccgccgacg cgcaggccct ggccgccacg
     3001 gcgccgccgg cgggccagcc gctggcgccc tggctgcagg cgcagcgtgc ccgcatcgtc
     3061 agcgagcgcg gcgcagccac gcgccacttc gaggaggccg agaaagcctg ctggcagcgc
     3121 tttgccgtca acgactgcct gcagcaggcg cgcgtgcagc ggcgcacggc gctggaccgg
     3181 ctgcgccagc aggaactcgc catcaacgat ctggagcgcc agcgtcaggc cgatgcgcgc
     3241 cggcttgaac ctagaaacgg cagttgaccg cttgtcatct tcgggcaggc cgcatccctg
     3301 cagggtttgg cggctccgat ggccttcacc gcctgggtga gcacgctgcg cacccacccg
     3361 caccgcgctc ggcgcgccat gcggcgttgc tccttcttgc gggcacgagc ccgcggcgtc
     3421 gttgcgtctt gcctggcgcg ccgatcacgg cacgggcggg cgcgtccaac tgccgtttct
     3481 aggaggctgt cgggcttgga gcgccgaaag cgttctcgaa gagcggcgta gccaaaatcg
     3541 gccccagcga gaccagtttt tgccggattt gcagcccaat agccgagcta ttgggcaaaa
     3601 agccggtgaa aaatgggccg ctgcggtcga tttgcagccg gcgatctcca agtccgacag
     3661 cctcctaggt tgaactggag cggcaggagt ccggcgcgac gcgccgatga gtgcatggcg
     3721 ctcaagccgc gaagcacggt gagcacatcc gcggcgcacc gctgaccgag ggggtcaacg
     3781 cgcccatgcc gggcgcgggc gcggtcagcg cagtggcgag aaggccgagc ccaccaccgg
     3841 ctggttggtc tgaagcggct cgaccttgac gatggccagc gccaggttgt cgccgccgcc
     3901 catggcgcgc gtgcgtgcct tgtcgatcag gaattcgctg gcctcgcgcg gtggcagcga
     3961 atgcagcacc gaacccagct cttccggcgt gaagtagtgc cacagcccgt cggtgcaggc
     4021 cagcagggcg tcgccgatct gcagctgggg gatgtagtgc acgtcgaact tgggcgcttc
     4081 ttcggtgccc aggcagccca gcagcacgtt ggacttggga tgcacggcgg cctcgtcttc
     4141 ggtcagctcg ccctggtcga ccagcgcctg cacgtaggac tgatccttgg tgcgacgcac
     4201 caggcgggcg ccacgaaaat ggtagagccg cgagtcgccg gtgtgcgccc aatggcattc
     4261 gccgcccacg ttgatgatga aggccgccat ggtgctgtgc ggctcctgct cggcggcgat
     4321 cgccgtcagc ttgatgatca ggtgcgcttc ctcgccgatc tggcgcagca ccgcggtggc
     4381 gtcttcgctg tcgggggcgt agcgctcgaa cagctggcgc gccgtcagca tcacctggtc
     4441 ggcggccttg cgcccgccgg tgcgcccgcc catgccgtcg gccaccacgc ccatcacgca
     4501 gccgttggcg cgcgcgtggg cgaacagggc cacctggtcc tgctggtagt cgcggtcgcc
     4561 cttgtgcaga ccggtggcgg cgctgagtcg atagcctttt tccatggtgc cccttgcaga
     4621 ggcggcgggg tacgccgcaa gtcgtattat tgaatgcggt ggcacggaag gcccggcgcc
     4681 gtgtcggcgg caagtgcaat ccttgtgctt tagtacgcaa tcttacacat gacggccaac
     4741 ctgcattccc ccgagcgccg gctcatcgag ctgcgcatgg agcatgccga tctcgacgcc
     4801 tcgatcgacc ggctggcggg tcaggcgccg ctggacgagc tgatgctgca acggctcaag
     4861 aagcgccgcc tggcgctgcg cgacgccatc gcgcggctgc aggcgcagct ggagcccgat
     4921 gaaccggcct gaacgcgtgt cgccagtggc cacccccggc gcgccgcaag ggcccgagcc
     4981 cggggcgctg gcgcaggcgg tcggggccgc cttcgacgat cacgggccgc tgtcgcaggc
     5041 ggccgagggc tttcgcctgc gcgagggcca gacccggctg gcgctggcgc tggcgcgggt
     5101 gatcgagcag ggcggggtgc tggtggccga ggccgccacc ggcgtcggca agacctttgc
     5161 ctacctggtg ccggccctgc tgtcgggcga gcgggtgctg ctgtcgaccg ccaccaagac
     5221 gctgcaggac cagctgttcg ggcgcgatct gccgcggctg gcgcgcgcgc tggggctgcc
     5281 ggtgagcatg gcgctgctca agggccgcgc cagctacctg tgcctgcacc gcctcgaaat
     5341 ggcgcatcag acaggggttt catcggcgcc tggtgtgcgc cagcagctat caaaaataga
     5401 gcgttggtcg aagatcacgc gcagtggcga cctggccgag ctgcctgatc tggacgagcg
     5461 ctcgccggtc atcccgctgg tcaccagcac gcgcgagaac tgtctgggcg ccgactgccc
     5521 gcagtttcgt gcctgtcacg tgcagctggc gcggcgccag gccatggcgg ccgacgtggt
     5581 ggtgatcaac caccacctgt tctttgccga tctggcgatc cgcgaatcgg gcatggccga
     5641 gctgctgccc agcgtgcgcg tggcggtgtt cgacgaagcg caccagctca acgaaaccgg
     5701 catccagttt ctcggccagc agctggccag cggtcagttg ctggactttg cgcgcgatct
     5761 gctggccgcc ggcctgcggc aggcgcgcgg cctgtgcgac tggcaggcgc tggccagcgc
     5821 ggtcgaggtg gccacgcgtg agctgcgcct ggcggccggt gcggggcgcg ccgccggccg
     5881 gctgcgctgg ctggaagcgg tgcccgaggg ggtcgatgcc gccgcctggc cgccggcgct
     5941 gcggcagctg caccaggcgc tggccgacgc cgccgccgcg ctggccacgg tcagcgagct
     6001 ggcccccgac ttcgtgcgcc tgcaccagcg cgcgctgctg ctggcgcagc gcgccgaacg
     6061 cttcggtcag ccgtgcgagc cggcgctggt gcgctgggta gacgttggtg cctcactgcg
     6121 gctggtggag tcgccgctgg acatcgccga caccgtgcgc acccgcctgc tggccgggcc
     6181 gcaggacgac ggccgcgcgc gcgcctgggc gttcgtgtcg gccacgctgg gcgacgacga
     6241 gcgcctgtcc tggttcaccg agccctgcgg cctgcaggac gccgaggtgc tgcgcatcgg
     6301 cagcccgttc gactacgcgc gccaggccgc gctgtacgtg ccgacccaca tcgcccgtcc
     6361 ggccgacccg gcgcacagcg ccgacgtggc gcgcctggcg ggcgacgcgg cggcccgcct
     6421 gggcgggcgc accatggtgc tgaccaccac gctgcgcgcg ctgcgccgca tcggcgagct
     6481 gctgggcgag cgcttcggcg ccggcgcgga catcgaggtg ctgatgcagg gcagcctgcc
     6541 caagcgcgtg ctggtggagc gctttcgtgc cggtggcagc gccggtcgtc cgggctgcgt
     6601 gctggtggcc tcggcctcgt tttgggaagg tgtcgacctg ccgggccagg cgctgcaatt
     6661 ggtggtgatc gacaagctgc cgtttccgcc cccgggtgac ccgctggtgg aagcgcgctg
     6721 ccgtcgcctg caagcgcagg gacgcagcgc ctttgccgac cattcggtgc ccgaggcggc
     6781 ggtggcgctc aagcagggcg cgggccggct gatccggcgc gaaagcgacc gcggcgtgct
     6841 ggtggtctgc gacacgcgcc tggtgcagat gggctacggt cgccgcctgc tggcggcact
     6901 gccgccgatg cggcggctgc cgtcgccgca ggccttcgag gccgcgctgg acgagctggt
     6961 cgccgaagat gctaccaaaa cgatagctgc tggcgcttga tgcgcaagcg ctggagccag
     7021 atctgacagc tattccgcga gatccgggct aggccgcccg ctcaccacag ctgccaccag
     7081 ggcttgtcgg cgccgcgcag gccgtgcgtc aggtaggtgc tgttggggta gttcctgtcg
     7141 agcacgcggc gcgcgtcgtc gcgcaactcg gtcatgccca gacggtcgta cgactgggtg
     7201 aggatgtaca gcgcctcttc cagcgacggc gcatcgcggt agtcggccag cgcctgctgg
     7261 gcgcggttga tcgccgccac gtaggcgccg cgcgtgaagt agtagcgcgc cacatgtact
     7321 tcgtattgcg ccagcgcgtt gatcgtgtag cgcatgcgcg cgcgcgcgtc gggggcgtag
     7381 cgcgaatcgg gaaagcgcgt caccagttcg ttgaacgatt cgaaggcttc cttggccgcc
     7441 ttctggtcgc gctcggacag atcctggcgt gccaggaaag agagcatgcc cagctcgtcg
     7501 ttgaaattga tcacgccctt gagaaacagc gcgtagtcga tcgccgggct ggccgggtgc
     7561 aggcgcatga agcgatccag cgtggctatc gcctcggcct tctggccgtc cttgaacagt
     7621 gcgtaggcct tgtccagctg cgcctgctgt gccagcgtgg tgccggcggc acggccttcc
     7681 agggtttcgt agagcgggat cgccttgtcg taggcaccgg cgttgagctc gtccttggcc
     7741 tcggcgtgga tgcggttggg gctccagccg gcggtccggt cgatcggctt ggacgagcag
     7801 gccgcaagca gcaccgccag cgccgccgcg acggcgcctg cgaccgataa tcgagcacct
     7861 tgcatgagcg gctaccttct tgaatcgagc aacggcaatt atatcgggca cccccccgtc
     7921 gaccgcaccc gctgacgccg accgggacgc cgacggcgaa ccgctggaag acgccggcgc
     7981 cgccgccgag ctgcgccagc tggcgctgcc ggccgagctg cacggccagc gcctggatcg
     8041 cgcgctggcc gcgctggtgc cggagttctc gcgcaactac ctgcgccagc tgatcgaagc
     8101 cggcgtggtg cacatgcagg ggcagccggt ggtcaagccg gcgcagaagg tgcgcgccgg
     8161 cgaccatctg gccatcgagc tgcgccccac gcccatgagc caggccttcg tgccgcaggc
     8221 catggcgctg gacgtggtgt acgaagacca gcatctggcg gtgatcgaca aaccggccgg
     8281 cctggtggtg cacccggcgc ccggccactg gagcggcacc ctgctcaacg gcctgctggc
     8341 gcgcgacgcc gccgccgccc acctgccgcg cgccggcatc gtgcaccggc tcgacaagga
     8401 caccagcggc ctgctggtgg tggcgcgcag ccgggcggcc atggacgcgc tggtggcgct
     8461 gatcgccgcg cgccaggtgc ggcgcgaata cctggcgctg gcgcaccggc cctggtccgg
     8521 cgcgccggcg cgcgagatcg acgccgccat cggccgcgac ccgcgccagc ggctgcgcat
     8581 ggcggtgctg gaccccgcgc gccatccggc caggccggcc cagacccgct tcgagctgct
     8641 gcagaacgcg ccccagggct gcctgctgcg ctgcctgctg cgcaccggtc gcacgcacca
     8701 gatccgcgtg catctggcac acctgggcca tccgctggtg gccgatgcgc tgtatggcgg
     8761 cgccccggcc gccgggctga cgcgccaggc gctgcatgcg cgccggctgg cctttcggca
     8821 cccgctgacc ggccagacgc tggatttcgt ggcgccgctg ccgaccgatc tgacgcacgc
     8881 actgagcgcc tggggcctta caatgcccgc tgacgacatg gacccggcgg ccagccccgc
     8941 gcgttgagtc catcacggcc cgcagcccgc ggcccctggc cggtgtggcc ggctcgcttg
     9001 ccgagcccgc gcgcaccggt ctccagacag acacggaaca ccctcgctgt gaattccacg
     9061 gacgccaagc gcatcctcga aaccgccctg atctgcgccc cgcagcccat gccgctgcgc
     9121 gaactgcgcg tgctgttcga cgacgaactg ggcgccgaca cgctgaagaa cctgctgctg
     9181 gagctgcagg acgactgggc ccagcgcggc gtcgagctgg ttcaggtggc cagcggctgg
     9241 cgcttccaga gccggcccga gatgcgcgag tacctcgacc gcctgcaccc cgaaaagccg
     9301 ccacgctaca cccgcgccac gctggagacg ctggccatca tcgcctaccg ccagccggtc
     9361 acgcgcggcg acatcgagga catccgcggc gtcaccgtca acacccagct catcaagcag
     9421 ctggaagacc gcggctgggt cgaagtcatc ggccaccgcg aaaccgtcgg ccgtcccggc
     9481 ctgtacgcca ccacgcgcca gttcctcgac gacctggggc tggagtcgct ggatcagctg
     9541 ccgatgctgg acaccccctc cgagcaggcc gccagcatgt tcgacgccct tgatcaggcc
     9601 gccgaggacg cgccggccgc tgccgccacc gacgccgagc cggccgccac gccggacgtc
     9661 gaggcgggtg agcccgccgc cgcgcccgcc gccaaggccg aacccgaatc cccatccgca
     9721 cccgacgccg acgacagcgc cgcgcccgcc gccaccccgg cgtcggcgcc cgagcccgag
     9781 cccgagcccg tggacgacag cccgccgtcc cgccccgaca ccgccgaaca cgaggccccc
     9841 catgtgccct gacgagccga ccgcgcctgc cgccgacgac caggcttcgg ccacccccgc
     9901 acccgcccgg cgatccagaa caaagccgac ttcggagccc gcccagactg cgccgaatgc
     9961 tattgatatg gtagcaatcg aggccgacgc cgaacccacc ggggcgccga cggccgcgga
    10021 cgaggcgccg gtcgtgatcg acgacgtgct gtccggccgc ttcgacgccg aagccgacgg
    10081 cggtgcgtcc gacgtcccgc ccaagcgcgt gctgctgccg cagcccgagc agcccaagct
    10141 gcacaaggtg ctggcccagg ccgggctggg ctcgcgcctg gagatggagc agctcatcct
    10201 gcaggggcgc atctcggtca acaaccagcc ggcccacatc ggccagcgca tccagttcgg
    10261 cgatgcggtc aagatcaacg gcaagccgat caagctgcgc atcgcgccgc cgccggcgcg
    10321 cgtgctggcc taccacaagc cggtgggcga ggtggtgacg cacgacgacc cgcagaaccg
    10381 ccccacggtg tttcgcaagc tgccgcggct gccgcagggc aagtggcagt cggtcggtcg
    10441 gctggacctg aacaccgaag gcctgctgct gctgaccaat tccggcgagc tggccaaccg
    10501 cctgatgcac ccgcgcttcg gcctggagcg cgaatacgcc gtgcgggtgc tgggcgcgct
    10561 gtccgacgag gaaaagcagc agctgctcga tggcgtgccg ctggacgacg gcatggcgca
    10621 gttcagctcg atccaggaag aagggcaggg cgaaggcgcc aaccagtggt accgcgtcac
    10681 catctccgaa ggccgcaacc gcgaggtgcg gcgcatgatc gagtccgtcg gtcacgccgt
    10741 cagccggctg atccgcatcc gctacggcgc catgatgctg ccgcgcggcc tgcggcgcgg
    10801 gcagtggatg gaactgggcg agcgcgacat ccgcgccctg ggtgcggcct cgggcacgcc
    10861 cgagcatgtg ctgcgcggcg gccgtgagcc cgggcccagg ccgtccgagc cgccgcgccg
    10921 tggtcgcggc gaccgcggcc gcggcagtgg gccacgcccc aacatggccg gtggcggccg
    10981 gccaccggcg aacaccggca gcggcaagcc gcgccggcgc gacggcgggc cggccggcgg
    11041 cgccggcgcg ccgcaacccg atcccatgaa gacctcgttc ggctacatcg gcgccgacag
    11101 cctgtcgcgc cagcgccaga gcgccggccg gggcgcaccg ggcgcgcgcg gcggtggcgg
    11161 ccgggcgcgc cgcggcccag ctcccggcgg ccggcgtggc tgagcggccg gcgccatcct
    11221 gcgaccgtcc cgcccgccgc cgcgaccgcg ggtgggccgg ttaaaatcaa tggctttgcc
    11281 cagccgggca gcagatcctt accgagaacc ttagaaagca cctccatggc tatcgaacgc
    11341 accctgtcca tcatcaagcc cgacgccgtc gccaagaacg tcatcggcca gatctacgcc
    11401 cgcttcgagg ccgccggcct gaagatcgtc gccgcccgca tgcagcacct gtcgcgccgc
    11461 gaggccgagc agttctatgc cgtgcacaag gagcgcccct tcttcaagga cctggtggag
    11521 ttcatgatct ccggcccggt gatgatccag gtgctggaag gcgagggtgc catcgccaag
    11581 aaccgcgacc tgatgggcgc caccgatccc aagaaggccg acaagggcac catccgtgcc
    11641 gacttcgccg actccatcga cgccaacgcc gtgcatggct ccgacgcgcc cgagacggcc
    11701 gccgtcgagg tggcgttctt cttctccggc atgggtgtct acagccgctg agccggcgca
    11761 aggcggcccg cgcagcgcat gaccacgccc gtcaacctgc tcgacttcga cctcgaaggc
    11821 ctggccgcct ggtgtgacac gctgggcgaa aagcgttatc gcgccaccca gctgtttcgc
    11881 tggatccatc agcgcggcgt cgccgagttc ggccagatga gcgatctggc caggtcgctg
    11941 cagggcaagc tgcacggccg cgccctggtg gcggcaccgg ccgtcatccg ccagcacgcc
    12001 tcgaaggacg gcaccatcaa gtggctgttc gacgtcggtg gcggcaacgc ggtcgaggcg
    12061 gtctacatcc cggaagacga ccgtggcacg ctgtgcgtgt cgtcgcaggc cggctgcgcc
    12121 gtggcctgtc gtttctgctc caccggtcac cagggctttt cgcgcaacct gagcacggcc
    12181 gagatcgtcg gccagctgtg gtttgccgag cattttctgc gccgccacct gaagaccgac
    12241 gagcgcgtca tctccaacgt ggtgatgatg ggcatgggcg agcccctgca gaactacgac
    12301 cgtctgctgc cggcgctgcg caccatgctg cacgaccatg gctatggcct gtcgcgccgg
    12361 cgcgtgacgg tgtccacctc gggcgtggtg cccatgatcg agcgcctggc gcgcgactgc
    12421 ccggtggcgc tggcggtgtc gctgcacgcg cccaccgacg cgttgcgcga ccatctggtg
    12481 ccgctcaacc gcaaataccc gatcgatgag ctgctgcgca cctgcaccgc ctacctggcg
    12541 cacgcgccgc gcgacttcat caccttcgaa tactgcatgc tcgacggcgt caacgactcc
    12601 gccgagcacg cccaggcgct ggtcgagctg atgcggcggc acgccgccag cggcctgcgc
    12661 ggcaagttca acctgattcc cttcaacccg ttcccgcagt cgggactgac gcgctcgccg
    12721 atggcgcgcg tgcaggcctt cggcaagctg ctgaacgacg ccggcatcgt caccaccatc
    12781 cgcaagacgc gcggcgacga catcgacgcc gcctgtggcc agctggccgg ggacgtgcag
    12841 gatcgcacgc gcgtgggcgt gcgcatcgcg cgccagcgca cggtggcgct gcaccagctg
    12901 gcgcccgcag gcgccgcccc ggcgccgaga aaggtgagtt catgagcctt catgcttgcg
    12961 ccccgagcgc ggcttcacgc cggcggctgc tggccctcgc cagcttcggt ctggcggcct
    13021 ggctgctggc cgcttgcgcc gctccgcccg gtctgggcga caacccgcag gagctgccca
    13081 ccgcatccga cgagaccgac gcccgtcggc gtgcccgcac ccggctggcg ctggccaccg
    13141 gttactacga gaacggccag cacaccgtgg cgctggacga gcagaaaaag gcgctgcagg
    13201 ccgacccgaa cttcggcgat gcctacaacc tgggcggtct gatctacatg gcgctgggtg
    13261 accaggcgct ggcgcaatcg catttccagc gcgccatcgc gctcaacccg cgcgacggcg
    13321 acgccctgca caacctgggc tggctgcaat gccagcaggg gcagctggcg gcggccaacc
    13381 agtcgttcga gcgcgcactg gcggtgccca cctacccggg ccgcgcgcgc acgctgatgg
    13441 cgcagggcgt gtgcgaggcg cgcgccggca acctggcgcg cgccgaggcg gcgctgatgc
    13501 gctcctacga gctggacgcc ggcaacccgg tgactgcctt caacctgtcg cagctgctgt
    13561 accagcgcgg cgactacacg cgggcgcagt tctacgtgcg gcgcctgaac aacagcgagc
    13621 tggccaatgc cgaatcgctc tggctgggca tccgcgtcga gcggcgtctg aacaaccgcc
    13681 aggccatgga gcagctggcg tcccagctgc gccggcgctt cccgcagtcg cgcgaactgc
    13741 tggcctacga gcgcggagcc ttcgatgact gaagcccaca ccgggccagc cagcctgcac
    13801 gacaccccgg cccacggcgc cggcccgcgt tcggccgggc agatgctgcg cgcgctgcgc
    13861 gaatcggcgg gcgtcgatcc ggcgctgctg gccagcgcca tgaaggtgtc gccgcagaag
    13921 ctcgacgcgc tcgaaaacga ccgcctggac gaactgcccg acgtcacttt cgcgcgcgcg
    13981 ctggcctcgg ccatctgccg tgccttcggc accgatccgg cgccggtgct ggagcgcatg
    14041 ccggtgtcgg cacccggtct gcgcccagtg gcgcaggcgc cgatcaacga gccgttccgt
    14101 cgcagcagcg acgggccggc gacggtgctg gcgggggggc tgtccaagcc gctggcgatc
    14161 ggtgtcatcg ccttgctgct gggggccgcg gcgctgtggt tgctgcccac gctgccgatc
    14221 cagctgaatg ctcccgcgcc ggccagtgag ccggccgcga ccgacggcgt ggtgcgcgaa
    14281 agcgtcatgc cgccggccgc caccagtgcc gagccacccg ctccgcccgc cccggccgaa
    14341 ctgtcggcca gcgcggcggc ggctgaacag gcgccgccgc cggccgctgc cgccccggcg
    14401 gcggccagca ccgatctgct gtatttcagc gctcaggccg aaacctgggt caccgtgcgc
    14461 gacgccagcg gcaagaacct gatcaaccgg gcgctgaacg ccggtgacag cgtgggcgtg
    14521 agcggtgagc cgccgctggc ggtcaccatc ggtcgcaagg atgcggtcga ggtgcgcgtg
    14581 cgcggcgagc cgttcgatca ccgctcgatc agcaagacgc cggtgtcgcg cttcgaggtc
    14641 cgatgagcat gccgtcaccc aactgcacac cgatcgaggt ggcccggccg gcgccgcgcc
    14701 gcacccgcca ggcgcgcgtg gtctggggcc ggcgtgtggt gacggtgggc ggcgacgcgc
    14761 cggtgcgcgt gcagtcgatg accaacaccg acaccgaaga tgccatcgcc accgccatcc
    14821 aggtcaagga actcgcgctg gccggcagcg agctggtgcg catcaccgtc aacacgcccg
    14881 aggcggcggc gcaggtgccc tacatccgcg agcagctcga ccgcatgggc atcgacgtgc
    14941 cgctgatcgg cgacttccac tacaacggcc accgcctgct gaccgagttc ccggattgcg
    15001 cgcaggcgct gtccaagtac cgcatcaatc ccggcaacgt cggcaagggc gccaagcgcg
    15061 acacccagtt cggccagatg atcgaggccg cgctgaagtg ggacaagccg gtgcgcatcg
    15121 gcgtcaactg gggcagcctg gaccaggacc tgctggccgg cctgatggac gaaaacagcc
    15181 gccgtgccca gccctgggac gcgcgccagg tgatgtacga ggcgctgatc agctcggccc
    15241 tgggatcggc cagccaggcg gtggagctcg gcatggaccc ggcgcaggtc atcctgtcgt
    15301 gcaaggtcag cggggtgcag gacctgatcg cggtctaccg cgagctggcc agccgcggcg
    15361 accatccgct gcacctgggc ctgaccgagg ccggcatggc gacgcgcggc acggtggcct
    15421 ccagcgtggc cctggcgatc ctgctgcagg aaggcatcgg cgacaccatc cgcgtcagcc
    15481 tcacgccgca gccgggcgag gcgcgcacgc aggaagtggt ggtggccagc gaaatcctgc
    15541 aggcgctggg gctgcgcgcc ttcgtgccca gcgtcaccgc ctgcccgggc tgcgggcgca
    15601 ccaccagcac cgtgttccag gagctggcca agcagatcga cgaccatctg cgcgcgcaga
    15661 tgccgctgtg gcgcagccag tatccgggcg tggaaaagct caaggtggcg gtgatgggct
    15721 gcatcgtcaa cggcccgggc gagagcaagc atgccgacat cggcatcagc ctgccgggca
    15781 ccggcgaggc gccggcggcg ccggtctaca tcgacggtga gaaagccatg acgctgcgcg
    15841 gcgaacacat cgcggccgag ttccagcaga tcgtggcgcg ctacatcgag cagcgcttcg
    15901 ggcgccggca accctgagcg gttttgagtc aaattcggct caaaccagca cccatcgtgc
    15961 gcaagcagct atcaaaacca tagttattca agtatgtctg gcagcaagca gatcaccgcc
    16021 atcaagggca tgaacgacat cctgccgccg gcgtcggcgc gctgggagtg gctggaggac
    16081 gtggtgcgcc gcgtgatggc gcagcacgcc taccgcaacg tgcgcacgcc catcatggag
    16141 ctgacgcagc tgtacacgcg cggcctgggc gaagtgaccg acatcgtcga gaaggagatg
    16201 tacgccttcg aggaccgcgc cgaccagcac ggccgcgccg accacctggc gctgcgcccc
    16261 gaaggcacgg ccggcctggt gcgcgccgtg gccgagcaca acctgctgta cgacggcggc
    16321 aagcggctgt tctacatggg ccccatgttc cggcgcgagc ggccgcagcg cggccgctac
    16381 cgccagtttc accagatcgg cgccgaggcg ctgggcttcg gcggccccga agtggacgcc
    16441 gaactgattc tgctggccgt ggcgctgtgg cgcgagctgg gcctgacagg ctggcggctg
    16501 gagctcaaca gcctgggtca gcccgacgag cggcgcgagc accgcgcggc gctggtggcc
    16561 tacctgcaag cccaccacga ccagctcgac gaggacgcgc gccggcgcct gcacagcaac
    16621 ccgctgcgca tcctcgacac caagaacccg gccatgcagg cgctggtcga gggcgcgccc
    16681 aagctgctgg actacctggg cgaggcctcg cgccggcatt tcgccgccgt acaggccatc
    16741 ctggcggccg ccggggtgtc gtggcacatc aacccgcgcc tggtgcgtgg cctcgactac
    16801 tacaacctga cggtgttcga gttcgtcacc gaccgtctgg gcgcgcaagg caccatctgc
    16861 ggcggcggcc gctacgacta cctgatcgag cagattggcg gcaagccggc gccggccgtg
    16921 ggctgggcgc tgggcgtcga gcgcgtgctg gaactgctgc aggaagaggg cgtgcacacg
    16981 ctggagccgg tgcccgacgt ctacgccatc atccccgacg ccgcggcgct gccgcaggcc
    17041 atgcgctgcc tgcagcagct gcgcgcgctc ggcgtggcgg tgcagatgca cgccggcagc
    17101 ggcgaaggca tgggcagcat gaagagccag ttcaagcgcg ccgacgcctc cggcgcgcgc
    17161 cacgcactga tctttggtgc cgatgagctg gccgccggcg aagtcaccgt caaggcgctg
    17221 cgcgacggcg ccggtgccca gctgcgccag ccgctggcac agccggacgc ctgggccgac
    17281 atcctaaaat cgaccgctta aacaccgaac caccacgcat ggccacccat ctcgacctcg
    17341 aagagcagga acagctcgac gccctcaagc atttctggaa ccgctggggc aacctcatca
    17401 cctgggtgct gatcgccgtg ctgggcgcct atgcggcctg gaacggctgg cagtactggg
    17461 agcggcgcca ggcgggtctg gcggcggtgc tgttcgacga ggtcgagcgc gccgcccagg
    17521 ccggcgacgc ggcgcgcgtg gagcgcgcgc tggccgacat caaggacaaa tttggcggca
    17581 ccacctacgc cgcccacgcc ggcctgcgcg gggccaggtt cttcgacgaa cagggcaaga
    17641 ccgccgaaac caaggccgcc ctggcctggg tggccgagaa ggcgcccaac gacgggctgc
    17701 gcgcggtggc gcgcctgcgc ctggcctcgg tccagctggg cgagaaggcc tacgacgagg
    17761 cgctcaggac gctggaggcc ggcttcccgc cgtcgttcgt cgcgctggcg gccgatcgcc
    17821 gcggcgacgt gctgctgcag cagggcaagc gcgagctggc ggcggccgaa ttcggcaagg
    17881 cctaccaggg catggcggcc gacaccgcgg gcgactaccg gcgcctggtc ggcatcaagc
    17941 tgaacgcgct gggcatcgac cccgacgccg gcgccaaggc gggagccgcg tcatgacggc
    18001 gcgcggtacg gtgcggcgcc ggatcgcctg cggcgccagc ctgctggctg cgcttgtgct
    18061 gacggcctgc gccggcggct caccgcggcc ccagccggcc gagttgccgg ccaacatcgc
    18121 cctgatcggc gtgcgccagg cctggacggc ccggctgccg gcgatcgact ttccgctgca
    18181 ggtggcggtg cagggccagc aggtcacgct ggccgccgcc gacggcagcg tcgtcaccct
    18241 ggacgccgct tccggccgtg aactcggtcg cgccagcgcc ggtgctccgc tgacggcggg
    18301 tgtgggcagc gatggccgcg tgagcgccgt ggtcacgcgc gacaaccgcg tggtggcgct
    18361 cgaaggcggt cgcgaactgt ggcgccagaa gttgggcaca cagagctaca ccgcgccgct
    18421 ggtggccggc gggcgcgtct tcgtgctcgg cgccgaccgc tcgctgagcg ccttcgacgg
    18481 ccgcaacggc gcccgtctgt ggcatctgga gcgtccggcc gagccgctgg tgctgcgcca
    18541 ggccggcgtg ctgcaggccg tgggcaacac cctggtggcc gggctggcgg ggcggctggt
    18601 gggggtcgat cccgacagcg gcagcgtgcg ctgggaggcg ccgatcgcct cggcacgtgg
    18661 caccaacgac gtcgagcgcc tgatcgacct ggtgggcggc gtcagccgcc acgagggcgt
    18721 ggtgtgcgcg cgggccttcc agacggcggt cggctgcgtc gacaccgcca gtggccgcct
    18781 gcgctggagc aaggcggcca gcggcgccac cggcgtggcc ggtgacggtc agctgctgtt
    18841 cggtgccgaa gccgacggcc gcgtgctggc ctggcgtgcc gacaccggcg accgcgcctg
    18901 ggtcaacgaa tccctgcagt ggcgccggct gacggcgccg ctggtgcttg gccgctcggt
    18961 ggtggtgggc gacggcaacg gcctgctgca tttcctgtcg cgtcaggacg gcacgccgct
    19021 ggatcgcctg agcaccgacg gtaccggcat cgccgccacc ccggtggcgg ccggcaacac
    19081 gctggtggtg gccacgcgcg gcggtacggt gtatggcttc gtgcccgaat gacatgggcg
    19141 ccgctgcaac ctgcctgcgc cgccggacca tgctggcggc gcctgcggac gccgccatgg
    19201 ccggcatgag ccgcgccgcc gccacgggct ggtgcggcag gccgcgctcc ggccccggat
    19261 cgacaccatg aaacccgtca ttgcgctggt ggggcggccc aatgtgggca aatcgacgct
    19321 gttcaaccgg ctcacccgca gccgcgatgc catcgtggcc gacttcgccg ggctgacgcg
    19381 cgaccggcat tacggtcagg gccacctggg caaacgcgaa tacatcgtca tcgacaccgg
    19441 cggcttcgag ccgcgggccg agagcggcat ctacaaggaa atggccaagc agacgcgcca
    19501 ggcggtggcc gaggccgacg tggtgctgtt tctggtcgac gcccgggccg gcctgtcggc
    19561 gcaggaccac gacatcgccg actacctgcg ccgcctgggc aagccctgcc tgctggtcgc
    19621 caacaaggcc gagggcatgc gcgagggggc gcagctggcc gagttctacg agctgggcct
    19681 gggcgcggtg attccggtgt cggccgcgca cggccagggc gtgcgcagcc tgctggaagg
    19741 ggcgctggac gcgctcgatc tgcccgacgt cgacgaagcg ccacccgacg ctgccgatgg
    19801 cgtgatccgc ctggcggtgg cgggccgccc caacgtgggc aagtccaccc tcatcaacac
    19861 ctggctgggc gaagagcgcc tggtggcgtt cgacctgccc ggcaccacgc gcgacgccat
    19921 cagcgtgccg ttcgagcgcg ccggtcgcca gttcgagctg atcgacaccg ccgggctgcg
    19981 tcgcaagggc aaggtgttcg aggcgatcga aaaattctcg gtggtcaaga cgttgcaggc
    20041 catcgaatcg gcccatgtgg tgctgctgct gctggacgcg acccagggtg tgaccgacca
    20101 ggatgcccac attgccggct tcatcctcga agccgggcgc gccgtggtgc tggcgatcaa
    20161 caagtgggac gcggtggacg actaccagcg cgagctggtg cggcgctcgg tggaatcgcg
    20221 cctgggcttc ctgaagttcg ccgagctgca cttcatctct gtgcgcaagc gccagggcct
    20281 ggggccgctg tgggcctcga tcgcgcgcgc ccaccaggcc gccatgcgca agctgccgac
    20341 accgcagctc acccgcaccc tgctggaggc ggtgcagttc caggcgccca agcgcgccgg
    20401 catgtaccgg cccaagctgc gctacgccca ccagggcggc atgaacccgc cggtgatcgt
    20461 ggtgcacggc aactcgctcg aaggcgtgac cgacacctac aagcgctacc tggaggggcg
    20521 ctttcgacag gtgttcgacc tcatcggcac gccgctgcgc atcgagatga agaccaccca
    20581 caacccctac gccacaaggc ctgaccaggg gtgaacggcc ccccatgccg gcttggaccg
    20641 gcctgccacg gccgctcggc ggcgcctttt ctacccggtt taccccttgt gctgggcggc
    20701 accaccccca tgtggtaagt tccatctctc ttcttacact ttccgaacac ggagaatatc
    20761 gtgagcaaca aagggcagct tctacaagac ccgtttctga acaccctgcg ccgtgagcac
    20821 atcccggtct ccatctatct ggtgaacggc atcaagctcc agggtcagat cgaatccttc
    20881 gaccagtacg tggtgctgtt gcgcaacaac gtcacccaga tggtctacaa gcacgcgatc
    20941 tccaccatcg tgcccggccg tccggtcaat ttctcggcca ccggggaagt cgcggaatcg
    21001 gattgatttt ggcctccagc gcccacccgg cgtgcgccag cagctattga ttcaatagca
    21061 atctggcgtt tctgacaagg cgctttgcca ctttgccccg taccgccacc cctgaacgcg
    21121 ctgccgccat cctggtcggg gtggatctgg gggtgcccca tttcgacggc caactgcagg
    21181 agctgggcct gctggccgaa accgccggcc tgcagccggt ggcgcgcctg acctgcaagc
    21241 gccaggcgcc cgatgcggcg ctgttcgtcg gccgcggcaa ggtcgaggag atccgcctgc
    21301 tggcgcaaag cgagggcgcg cgcgaagtgc tgttcgacca ggcgctctcg ccggtgcagc
    21361 agcgcaacct ggagcgcgcc atcggcctgc cggtcaacga ccgcaccctg gtgatcctcg
    21421 aaatcttcgc ccagcgcgcg cgcagccacg agggcaagct gcaggtcgag ctggcgcggc
    21481 tgcaatacct cagcacccgc ctggtgcgcc gctggtcgca cctggagcgc cagcagggcg
    21541 gcatcggcgg gcgcggcggc ccgggcgaaa cccagatcga actggaccgc cgcatgatcg
    21601 gtgaagccat caagcgcacc aaggatcgcc tgcagaaggt caagcgccag cgccagactc
    21661 agcggcgcca gcgcgagcgg cgccacacct tcaacatctc gctggtcggc tacaccaacg
    21721 ccggcaagtc gacgctgttc aacgccctgg tgaaggcgcg cgcctatgcc gccgaccagc
    21781 tgtttgccac gctcgacacc accacgcgcc agctctacct gggccaggcc gggcgttcgg
    21841 tatcgctgtc ggacacggtg ggcttcatcc gcgacctgcc gcacggcctg atcgaggcct
    21901 tcgaggccac gctgcaggag gcggccgacg ccgacctgct gctgcacgtg gtcgacgccg
    21961 ccaaccccgg tcacctggag cagatcgagg aggtgcagcg cgtgctgcgc gaaatcggcg
    22021 ccgccgagct gccgcagctg ctggtgttca acaagctcga cgagctggag ccggcgcgcc
    22081 gcccgctgca gctcaaggac agcgtgacgc tggacggccg cgagctgacg cgcattttcg
    22141 tcagcgcgcg cagtggcgaa ggcctgccgc tgctgcgtgc cgaactggag gcggccgtgc
    22201 agagccaggc gctggaagcg ggcggcgacg gggtgccggc gccgattggg cacaatgatg
    22261 ggttgcacag ccgagaagtc gacgcatgaa tgaccgcacc agtgccccgc gaccggcctc
    22321 atggccgggc cggatccggg gcatgttcaa cctccatgac ccgcgctggg ggcgcgacga
    22381 cgaaccgtcg gggcaggagc cgggccagcg cccggacgag ccccagcggc caccgccgcg
    22441 tggccagggc ccgcaggccg gaccgcccga cctcgacgag ctgtggcgcg acttcaaccg
    22501 caagctgggc ggcctgttcg gcggcgggcg cgggcggggt cgcaacccgt actcgggtgg
    22561 cggcatgccg cccgagatga agggccgcgg cgccggcatc ggcatcggcg tgatcgccgc
    22621 cgtggcgctg gcgatctggc tggcctcggg cgtgttcatc gtgcgcgaag gcgagcaggc
    22681 cgtggtcacc accttcggtc gcttcgacgg catccgcaag gccggcctga actggcgcat
    22741 gccgtacccg atccagcggc acgagacggt gcgggtgacg cagatccgct cggtcgacgt
    22801 ggggcgcgac agcatcatcc gcagcaccgg tctgcgcgag tcggccatgc tgacgcagga
    22861 cgagaacatc gtcgagatca agttcgccgt gcagtaccgc ctgaacgacg cgcgcgccta
    22921 cctgttcgaa agcaagagcc cggacgacgc cgtggtgcag gccgccgaaa gcgcggtgcg
    22981 cgaagtggtc ggcaagatga ccatggacac cgccatggcc gaggagcgcg accagatcgc
    23041 cccgcgcgtg cgcgacctga tgcaggccat cctcgaccgc taccaggtgg gcatcgaggt
    23101 ggtggccgtc aacatgcagc agggcggggt gcgtccgccc gagcaggtgc aggcggcgtt
    23161 cgacgacgtg ctcaaggccg gccaggagcg cgagcgcgtc aagaacgagg cccaggccta
    23221 cgccaacgac gtggtgccgc gtgccgtcgg taccgccgcc cgcctgaagc aggaggccga
    23281 aggctacaag gcccgcatcg tggcgcaggc gcagggcgat gcgcagcgct tctcgtcggt
    23341 gctgagcgaa taccagaagg cgccgcaggt cacgcgcgag cgcatgtacc tcgacgccat
    23401 gcagcagatc tacggcggcg tcaacaagat catcatcgac agccgcgccg gcggcaacct
    23461 gatgtacctg ccgctggaca agctgctgca gggtgcagca ggcgcgggcg cggcggccgg
    23521 cgcggcgccg gccgccgccc aggcaccggc gccggccacc gccccggctg cgcccgccgc
    23581 cgacccgcgc gcgcgcgaca acgcccgcac gcgcgagcgt gaaacccgct aagtcaggcc
    23641 gcaccccatg aacagagtcg gattcatcgg aaccaccgtc ctcatcctgc tggccctgct
    23701 gggctcgacc gtgttcgtgg tcgaccagcg ccagttcggc gtggtctacc agctcggtca
    23761 gatccagcgc gtcatcaccc agcccgggct gaatttcaag ctgccgccgc cgatgcagaa
    23821 cgtcacctac atcgacaagc gcctgctcac gctcgacagc atggacaccg agccggtgct
    23881 gacggccgag aagcagcgcg tggtgatcga ctggtacgtg cgctggcgca tcaccgaccc
    23941 gcaggcctac atccgcaacg tcggcctgga cgaaagcgcc ggcgccctgc agctcaaccg
    24001 cgtggtgcgc aacgccttcc aggaagaaat caaccgccgc accgtgcgcg agctgatctc
    24061 gaccaagcgc gaaaccctga tggccgacgt caagcgtgaa gtgtcggacg ccgtcaaggg
    24121 cgccaagccc tggggcatcg acatcatcga cgtgcgcatc acgcgcgccg actacgtgga
    24181 atcgatcacc gagtcggtct accgccgcat gcaggccgag cgccagcgcg tggccaacga
    24241 actgcgctcc accggcgccg ccgagggcga gcagatccgt gccaacgccg accgccagcg
    24301 cgaagtgacc gtggccgacg cctaccgcga agccgagaag gtgcgtggcg agggcgacgc
    24361 ccaggccacc cgcatctatg gcgacgcctt cggccgcgac ccgcagttcg ccgagttcta
    24421 ccgcagcctg gacgcctaca aggccagcat cggcaagaag ggcgacgtgc tggtgatcga
    24481 tccggcctcg tcggacttct tccgcaacct gcgcggcgca ccggcgcggc gctgacaccc
    24541 gggggtccga cggtggacgg tgccacctgg ctggccgcgc tcggcctggc cctggtcctg
    24601 gagggcctgg tgccgtttct ggccccgcgc gcctggcgtc aggccttcag ccagttgctg
    24661 caactgcgcg acggccaggt gcgcttcttc ggtctgctgg cgctggcggg cggtctggcc
    24721 ctgctgtggc tggcgtgacg gcgggccgcg cgcagccacg ccggtagaat cgccttttca
    24781 acagccgacg cgaaagctgc cccagccatg tccgcctggc tcctgccgga ccacatcgcc
    24841 gatgttctgc cctccgaggc ccggcacatc gaagaactcc gccgcgaact gctcgacacc
    24901 gcccgtggct atggctacga gctggtgatc ccgccgctga tcgaacacct ggaatcgctg
    24961 ctcaccggca ccggcgaagc gctgggcctg cagaccttca agctggtcga ccagctgtcg
    25021 ggccgctcgc tgggcgtgcg cgccgacacc acgccgcagg tggcacgcat cgacgcccat
    25081 ctgctgggcc gtcaggccgt cacgcggctg tgctactgcg gcccggtgct gcacacccag
    25141 ccggagcgcc cgcacgccac gcgcgagccg ctgcagctgg gcgccgaaat ctacggccac
    25201 gccggccgcg aagccgacct cgaaatcctg tcgctggcgc tggactgcct gcgcgccgcg
    25261 ccggtccatg gcctgtcggt cgacctggcc gacgcgcgca tcgtgcgcgc cctgctggcc
    25321 ggcgtgctgg tcgaccccga gcgcctgacc gaggtgcatc tggcgctggc cgccaaggac
    25381 gccagcgaac tggcccggct gaccgcggcg tttcccgcca gcgcgcgcca gggcctgtgc
    25441 gcgctggtcg gcctgtatgg cgatgcgggt gtgctggatg aggccgaaaa agccctctcg
    25501 ggcgcacctg gcgtgcgcga ggtgctatcc aatctgaagt ggctggcgag ccagctggag
    25561 ggtgtgcggg tcagcttcga tctggccgac gcgcgcggct ggggctacta caccggggcc
    25621 cgctttgccg tctatgccga aggggcgggc gacgcgctgc tgcgcggcgg ccgctacgac
    25681 ggcgtaggcg cggtgttcgg ccacaagctg gaccgcgaac ggccggctgt cggcttcagc
    25741 ctcgacctca agcaactggt gggcgtggcc gcgccgcgcc cgctgcgtgc cgccatccgc
    25801 gccccctggg gcgatgcccc gggcttgcgc aaggcggtgg cggggctgcg ccggcgcggc
    25861 gaaaccgtgg tctgcgccct gccgggccac gccgaccaga tcgacgaatt tcactgcgac
    25921 cgcgagctca tcgaggccgg cgggcagtgg gtgatccagg cgctcggatg agcccgcctg
    25981 cccgatccgc tgcgcgccgc cacggcggtg cgcgccgggc cacgccgctg gccggccggc
    26041 ccgatcccga cccccgaatc cacagcaatc aaggcaaagc atgaaaccct ccaccaccgg
    26101 acgcaacgtc gtcgtcgtcg gcacccagtg gggcgacgaa ggcaagggca agctggtcga
    26161 ctggctgacc gagagcgcgc agggcgtggt gcgcttccag ggcggccaca acgccggcca
    26221 caccctggtc atcaacggcg tcaagaccgc gctgcacctg atccccagcg gcatcatgcg
    26281 cccgggcgtg acctgctaca tcggcaacgg cgtggtgctg tccgtcgcca agctgctgga
    26341 agaaatcgcc ggcctggagc gcgccggcgt gcaggtgcgc gagcgcctgc gcatcagcga
    26401 aggctgcccg ctgatcctgc cgctgcacgc cgccatggac gtggcgcgcg aagccgcgcg
    26461 cgagcagggc ggcagcgaga agatcggcac caccggccgc ggcatcggtc cggcctacga
    26521 ggacaagatc gcccgccgcg ccctgcgcgc acaggacctg aaacaccccg agcgcttcgc
    26581 cgtcaagctg cgcgagctgc tggcgctgta caaccatgag ctgctgggct atctgggttc
    26641 ggcgcgcatc gccttcaacg aggcgctgac gccctacctg aaagacggcg cactgcagtt
    26701 cgagcccatc tacgacgagg ccatgcgcca ggccgagcag gtgcgcccca tgctggccga
    26761 cgtctcgcgc gagctgaacg aggcccacca gcagggcgcc aacctgctgt tcgaaggcgc
    26821 ccagggcacg ctgctggacg tcgaccacgg cacctacccc ttcgtcacca gcagcaacac
    26881 cgtggccggc aacgccgcgg ccggcgctgg cgtcggcccg ggcctgctgc actacgtgct
    26941 gggcatcacc aaggcctact gcacgcgcgt cggcggcggg ccgttcccga ccgagctgga
    27001 ctggcagacc cccggcaccc ccggctggca catgagcacc gtcggcgccg aaaaaggcgt
    27061 caccaccggg cgcagccggc gctgcggctg gttcgacgcc gcgctgctca agcgctcggc
    27121 ccaggtcaac ggcctgtcgg gcctgtgcat caccaagctg gacgtgctcg acggtctgcc
    27181 cgagctgcag ctgtgcaccg gctacgaact ggatggcgag acgatcgaca tcctgccgct
    27241 gggcgccgac gacatcgcgc gctgcaagcc gatctacgaa accatgcccg gctggagcga
    27301 aagcacggtc ggcgccaccc gctacgagca gctgccgatc cacgcgcggc tgtacctgca
    27361 gcgcatcgag catgtcaccg gggtgccgat ccacgtcgtt tccaccagcc ccgatcgcga
    27421 ccacacgatc atgctgcggc accccttcct gcctacctga tacaggccaa atcaccttgc
    27481 agcgcccgcc agaacggcgc caccagctat caaaagagga gagacccgca tgttgaccga
    27541 agacggcaaa cacttgtacg tgagctacga cgagtaccac gccctgatcg agaagctggc
    27601 gctcaaggtg caccagtcgg gctggcggtt cgacaccatc ctgtgtctgg cgcgcggcgg
    27661 catgcgcccg ggcgacatcc tgagccgcat cttcgacgtg ccgctggcca tcatgtccac
    27721 cagcagctac cgcgccgagg ccggcaaggt gcagggccat ctggacatcg cgcactacat
    27781 caccacgccc cagggccaga tcgccggtcg ggtgctgctg gtggacgacc tggccgacac
    27841 cggcgtcacg ctgcaggcgg tggtcgagcg cctgcgcaac gactacccgc ccatcaccga
    27901 attgcgcacc gccgtgatct ggaccaaggg cagctccagc tttcaggccg actactcggt
    27961 cgagcacctg cccaccaacc cctggatcca ccagcccttc gagagctacg acggcctgac
    28021 gcccgacaag ctgctggcca aatggaaggt gtgagctgca gggcaggcgg ggcgacggga
    28081 gttacctgtc gcccgccatc gatgacctgc gccgtgtgct cgttgcgggt tgctgacccc
    28141 gggcacgctg gtctgaatgc ctgcgtgccg ggctatgaag ttcagcgcct gctggcgcgc
    28201 cgcaccagca ggtctgatta gaagccccga cccagcctca tgccagatcg acgcatcgcc
    28261 atcaccccca gcaacaggct gagccccagc agggcgatct tgcctggtcc gggcacgggg
    28321 gccggagccg ccagggcagc agcctggggc gcggcccact cgctcgatgg accgtaactc
    28381 gtggcaccgc tgctccaggt ggcgccgttg ttggcggccc agctgcccgg cccggtcacc
    28441 gtgtagttga agcggtagta gccgttgtag gtcggattgc tgggctggtc attcaggaag
    28501 atgcctctga gcgtgccgct gaagctgctg ggcatggcac tggtttcgta ccagccggtg
    28561 cttccgttca gcgtggcggg gctggcaaag ttggcagtca cgtccagatg gaagtcggcg
    28621 aacgcgccac gcgaaatcgc agccgcaccg ctgtccggtg cgggccacag gcgaccggcg
    28681 tcgagatcgt cgccatggcc accgctgacg gtcagccgga attgagtgtg accgggctgc
    28741 cagccgcctg tcaccgtggg gctgctttcc tggtagtact ggatgaagcc gtcgttggtg
    28801 ttgttcgggt tgccccagcc gttgccactg gcgggcacca gggtgaagta ccagggagtc
    28861 gacatgtagg tcaggtccga cccatatgcg gacggtgcct ggctggtcac cgagatctgc
    28921 ccgtcgcgcg cgttgggcag ggttgagttg gcgccgttgg tcgccgtttg aatggtgtag
    28981 ggcccgccgg tcggattggt ttgattctgg gcatcggcca gcgtggcgta gcgggtgacc
    29041 gtaccgctgt aggtgaagtt gtcgaacgag acgctgagtg gcactgcctg agcccaggct
    29101 gcccaagtca gcgcggtcgc acccagcagc gtgcggaatc tggttctgtc aaacatgaaa
    29161 cctccttgga aacaattgga atgacaatac ttgtgccctg cgtcttggcg caggcttcac
    29221 cagggcgtgg caagggccga cgaattgcga tcgttcgacc gatgcgttag gctgaactgt
    29281 ttaatggtat caaatagtgt cgcggacaaa tcggctctgg cgctcgcgag tctcggtgca
    29341 tgatctgagt caaattcgag accgcgtggt caccagtcct gagttgcccc tgccccacga
    29401 tcgccccatg accgacccca tcacccagct gatccaccac ccctaccagc cgccggccgg
    29461 ctttgcggcg ccgcagccgg ccgtgcacaa ggcctcgacg gtgttcttcc cgaacgtggc
    29521 cgccatgcgc gagcgcgact ggaagcacaa gagcggctac acctacggcc tgcacggcac
    29581 gcccaccacc ttcctgctgg aagagcgcat cgccaccctg gaaggggggc tgcagtgcgt
    29641 gctggtgccc agtggcctgg ccgccatcgc caacgtggcg ctggcgctgc tgcagaccgg
    29701 cgacgaggtg ttgatccccg acaacgccta cggccccaac aaggcgctgg ccgagggcga
    29761 actggcggcc tggggcatca cgcaccagta ctacgacccg atggatctgg ccgatctggc
    29821 ggcgcgcatc ggcgagcgca cacgcctggt gtggctggaa gcggccggct cgctgacgct
    29881 ggagtttccc gacctgatcg ggcaggtgcg cctgtgccgc gcgcgtggcg tgccgtgcgc
    29941 gctggacaac acctggggcg ccggcctggc cttcaacccg ttcgatctgg acggtcaggg
    30001 cctgggggtg gacctgagcg cgcacgcgct gaccaagtac cccagcggcg gcggcgacgt
    30061 gctgatgggc agcgtcacca cgcgcgacga ggcgctgcac ctgaagctca agctgaccca
    30121 catgcggctg ggcctgggcg tggccgccaa cgacgccgaa gcggtgctgc gcgccctgcc
    30181 cagcgtggcc ctgcgctacc gggcgcagga ccaggccgcg cgggcactgg ccggctggtg
    30241 cgcgcagcag tcggcgttcg cccaggtgct gcacccggcg ctggccgatg cgcccggcca
    30301 tgcccactgg cagcaggtgt gcggcgccgc cgacgctcgc gggcagggcg cggcggccgg
    30361 cctgttcagc gcgatcgtgc acgagcgcta cagccaggcc caggtcgacg ccttctgcga
    30421 cacgctcaag cggttcaagc tcggctacag ctggggcggg ccgatcagcc tggtggtgcc
    30481 ctacgacctg gcggccctgc gcgcgcgcgg ctggccggcg cacctcaggc gcggcacgct
    30541 ggtgcgcttt tccaccggcc tggaggcgct ggtcgacctg caggccgatc tgcagcaggc
    30601 gctgaaccgc gccctgccgt cctgaagcta tagtttatgt agctgttggc gcttgttggg
    30661 cgggcgccag agtgcgattt gatgctgcgt cgccggtgtc aagcaggcgc ccgggtcatt
    30721 acccattgag tgggcatggt tttttggcta tcttgccgcc cgagcaccgc ccttcggcgg
    30781 tcacccggtc acccatgagc cagcccgaca ccgatcccac cgccgccccg ccggccgccg
    30841 ccccggcgcc ggcctatcag ccctacgtgc cgcccccggt caccaccccg caggccatca
    30901 tggtgccgct gggcctgtcc gacccgctgc gctggctggg gcgcggctgg cgcgacttca
    30961 tggccgcgcc gggcatcggc ctgttctacg gcgtgtgctt ctggctgatg gcggcgctgc
    31021 tggcgctggt gtttcgcagc aagcccgaat acgtgatgtc gatcgcctcc ggctgcctgc
    31081 tggtggggcc gttcctggcc atggggctgt acgaggtcag ccgccggcgc gagcagggcg
    31141 tgccgcccga gctggccgcc tcgctcacct gctgggaccg gcacgtgcgc agcatgggca
    31201 tgctggtgct ggtgctgatc gtgctggagc tgctgtgggg ccgcgcctcg ctggtggtgt
    31261 tcgcggtgtt cttcaacacc ggcatgccgt cgaccactgg cgtgctgcag gccatcttca
    31321 accccgacaa cctcgaattc gtgctggtct acctgaccgt gggcggcttc ttcgcgctgc
    31381 tggtgttttc caccacggtg gtgtcgatcc ccatgatcct cgaccgcgac accgacgcca
    31441 tcagcgccgc catcaccagc atccgcgtgg tgttcgccaa cccgctgacg ctgctgtggt
    31501 ggggcctgct gatcaccttg ctgatcgcgc tgtcgctgct cagcctgggt ctgggcctgc
    31561 tgctggtggg gccgctgctg gggcacgcca gctggcatgc ctaccgcggc gccgtgcagt
    31621 ggcgcgagcc gcccccgcgc ccggccgacg cctgcgtgcc ggccccggtg ccggcgccgg
    31681 cgccgctgcc cggctgaacg ccgtcaggcg gggtggccgg cgcctggctg gccaggatca
    31741 gcgcgcaggc ggcgcgcagg ctggcgggcg agcggtcggc cagcggcacc gccagggcct
    31801 ggccaaagcg cgcgaagttg cgctgcgtcg agctggcgta gcccgggtcg tagtgccggg
    31861 ccagcagctc acgcaccacc tgatcggtgt ggccggcggc cacgcgcgcc ttccagtcgt
    31921 cgatcacggc gcgcccgcgc agcgccacca gggtgtccag gcgctggcag aagaaatccg
    31981 ggtcgcggac gaagaagggg tagtcctcca gcagcagcgc cacccgctcg tcgtcggcca
    32041 gctcgatgcg caggcagtcg ctggcgcgca tggcggcgat cagcgcctcg ggcacggtga
    32101 cgttgccgac cttcttgctc tcggcctcca cgtacaccgg tcgcgccggc tccaggtggc
    32161 gcagcgcgtc ccacacccgc atctcgaagt gcttctgcga cggctgcggc tggcccggaa
    32221 tcagccccag caccgaggcg cggtgctgcg ccagcccttc caggtccagc acctgcgcgc
    32281 cggccgctgc cagcgcgtgc agcaggcgcg tcttgcccga gccggtgggg ccgcacacca
    32341 cgcgcaggcg caggcgctgc acctgcgccg gaatgtcggt caggagggcg acccggaaag
    32401 ctttgtagcc gccttcgatc agcgtcacct gaaagccgat ctgtcccagc accagcgcca
    32461 gcgcgccgct gcgctgaccg ccgcgccagc agtacagcag cgggcgccag cggcgcgagg
    32521 tgtgggccag ctcgcgctcg atgtggtcgg cgatgttgcg cgccaccagg gcggcgccca
    32581 gcttgcgcgc ctcgaacggg cttgcctgct tgtacagcgt gcccacgcgg gcgcgctgct
    32641 ggtcgttcag gctgggccag ttgacggcgc ccggcaggtg gtcgtgcgcg aattcgcttt
    32701 cgctgcgggc gtcgatgatg gcgctgaact cggccagtcg cgccagggcg tcggcggcgg
    32761 cgatggtctg gacgctcatg gtttcagcag ccggcgcagc tcgggccaga cgttgtcgag
    32821 gatgatcggg tgcgccgccg ccgtggggtg gatgcggtcg ggctggaaca gcgcttgggc
    32881 gtggtcgcca tcggccacgc ccttgagcag aaacggcacc agcgccgccc gctgcgcctt
    32941 ggccacctgc gggtagagcg cgaagaagcg ccgcccgaag tcggcgccgt agttgggcgg
    33001 catttgcatg ccggccagca gcaccttggc gccggcggcc tgcgccgcct gggtcatggc
    33061 ggtcaggttg gcctcggtca tctccagcga cagcccgcgc agcgcgtcgt tgccgcccag
    33121 ctcgatcacc acgacggccg gccggtgcgc cttcagcagc gccggcaggc gcgagcgccc
    33181 gcccgccgtg gtgtcgcccg agacgctggc gttcagcacg cgggcggcga tctgctcgcg
    33241 cgccaggcgt tgctccagca gcgccaccca gccgctgccg cgggcgatgc cgtattcggc
    33301 gctgatcgag tcgcccagca ccaggatcag gggcggcgcg gccgtctgcg cggccaggcc
    33361 cgccggcgcg gcggccagca ggaagcggat acagtctcga cgattcaaat cggatgcctt
    33421 ttcatgacgg atgtttcgca cggggcgcca gcgggcgccc gccgcgctcc catcatcgcg
    33481 gtggagcacg tgttcaagtc ggtcaccgac tccagcggca cgctggatat tctgcgcgat
    33541 atccacttca gcctggccga gcgcgagacg gtcgccattg tcggcgcctc cggctcgggc
    33601 aagagcaccc tgctgtcgat catcgccggg ctggacacgc ccacccgcgg cacggtgcgc
    33661 attgccggcg acgacctgtt tgccgtctcc gaagacgcgc gcgcggcgct gcgggcgcgt
    33721 cgcatcggct tcgtgttcca gagctttcag ctgctgggca acctgacggc gctggaaaac
    33781 gtgatgctgc cgctggagct ggccggccgg cgcgacgccc gcgccgccgc caccgagatg
    33841 ctggcgcgcg tgggcctggc cgagcgcctg cgccactacc ccaaggtgct gtcgggcggt
    33901 gagcagcagc gcgtggcgct ggcgcgcgcc ttcgtgctgc acccggcggt gctgctggcc
    33961 gacgagccca ccggcagcct cgaccacgcc accggcgaga aggtgatggc gctgatgttc
    34021 gagctcaacc gcgagcaggg caccaccctg gtgctggtca cgcacgaccg cggcatcgcg
    34081 gcgcgctgcc agcgccggct gaccatcgac gccggttgca tcgcggccga cgagcaagct
    34141 gaatcgctat cgataaagta gctgctggcg ctcaccagac gggcgctgga gcctgatttt
    34201 ccttagagct ggaaggcgag ggcgccgacc acaccttcca gcacgtcgcg cagcaggccg
    34261 ggcggccggg cggaggccgg ctcgccgtcg gcggcggtgc gggtgatcca gccggccagc
    34321 cagtcgatct cggcgcggct gtagaaggcg gccatggcct cgtagttgat gaacaggctg
    34381 cggctgtcca ggttggccga gccgcacagc gccagcgcct ggtccaccac caccgccttg
    34441 gcatgcagca tggtgggcag caggcgcacc tcgacgccgt gctccaccag cgcgcgcacc
    34501 gcgcgtccgc gcgcccagtc ggccagccgg tggttggagc ggcgcggcag cagcagcgtc
    34561 acgcgcacgc cgcgcttggc cgccagcagc agcgcttcca gcagcccctc gtccggcacg
    34621 aaatagggcg tggccagcag caggcgctgc tgcgcatgga aggcggccga caccagcagc
    34681 gcgtgcagca cgtcctcatg gaaatccggc ccgctgggca cccagtgcgc cagcgccacc
    34741 tgggccgacg gcgctgcgcc gctggcggca gggggcgcgc tggccagcgc ggcggcgctg
    34801 gcgtcggccg gctggtaggc gtgcagcagc gcctcgtggc gcagcgcgcg ctcggcatag
    34861 ggcacgcccg aggcgcgccg cagccccagg gcgatgcgcc agtcgccttc gaacagcgcc
    34921 atggcctgcg tcgccagggc gccctcggcg ctgaagctga ggtcgatcca gggcgcctcg
    34981 cccgcccggc cgatgaagta ttcgtcggcc aggttgcgcc cgccgcccca cagacgcagg
    35041 ccgtcggcca ccaccagctt gcggtggttg cgcaggttgg tgcggccgcg ccgcggattg
    35101 cgcagcgccg gcatgaagcg gcgcacgcgc accccggcgg cgcgcagccg ggcgtcgtgg
    35161 ctgcgccggc tcctgaggct gccgacgctg tcgaccagca ggcgcacgcg cacgccggcg
    35221 cgggcgcgcg cggccagctc gtcggccacc gccgcgccta cctggtcgtc gcccagcaca
    35281 taggtgcaca cgtccagcgt gcgttcggcc gacgcgatga gcgcgcgcag ctcggccagc
    35341 gcctgcgcgc catcggcatg aaagcgcagc gccgcctgcg gccgcgcctc gggcacgccc
    35401 agcgccgcca gcaggccggt ggcccaggcc ggcgccagcc gcgcccaggg gccggccggc
    35461 accggctggc gcggggtgct cgggcgcacc gtcttgcgcg tgccgaacag cagaaacagc
    35521 ggcaccccca gatagggaaa ggccgcaatc cccagcaccc aggccagcgc cgcgtagggg
    35581 tggcgccgct gctgacgcgc ccgcgtcacc agcacgtaca gcagcaggcc ggcggccacg
    35641 aaggcggcat gttccagcgg acttagcgtg ggcagacgga tcatgcgaaa cgttcagggg
    35701 cttcgggttg ctcttgaaaa catagcgtgc ggcgcccggc aggtacggcc ggcaggccgc
    35761 aatcggtctg attatgcggc gcggcgcgtg gcatcggtgc ggtgggatgc cccgcccggg
    35821 ccgctcgcat gctattgaat aactagctgc tggcgctttg ccggtgcgcg ccgcagcccc
    35881 aaaacgctca ccaaccgggg gcgccgccgg gcaccgataa tcaccccatg tccaccccca
    35941 aagtgctgtt cggcttccat gccgtcggcg tgcgtctgaa gaccgcgccc gcctccgtca
    36001 tcgaggtcta tgtcgatccc ggccggcgcg acgcgcgcat gaagcagttt ctggcgcgtg
    36061 cggccgaagc cggcgtgcgc gttatcgagg ccgatggccc gcgcctggcc cggctgtgcg
    36121 gcggcgccgg ccaccagggc gtggtggcgc gcgtgcagcc gatcgcgcag acgcattcgc
    36181 tggacgaact gctggacggc gtggccgagc cgccactgct gctggtgctc gacggcgtga
    36241 ccgacccgca caacctgggc gcctgtctgc gcgtggccga tggcgccggc gcgcacgccg
    36301 tcatcgcgcc caaggaccac gccgccggca tcaacgccac cgtcgccaag gtggccagtg
    36361 gggcggccga aaccgtgccc tacctgatgg tcaccaacct ggcgcgcacg ctgggcgagc
    36421 tcaaggagcg cggcatctgg gtcatcggca cctcggacga cgcgccgcag accctgtacc
    36481 aggccgacct gaagggcccg accgctctgg tgctgggcgc cgaaggcccc gggatgcgcc
    36541 agctcacgcg caagagctgc gacctgctgg tgcgcattcc catgcacggc gcggtggaga
    36601 gcctgaacgt ctcggtggcc agcgcggtct gcctgtatga ggcgcggcgc cagcggggct
    36661 gaagcggctt cgggttcaag attcgtgacc catgacccca tgaccgatgg accgtgggtc
    36721 attgaaccta gaaacagcag ttgaccgctt gccaacttcg ggcagaccgc atcgatgcgc
    36781 ggtctggcgg ctgcgatggc cttcaccgcc tgggtgagtc cgctgcgcac ccacccgcac
    36841 cgcgctcggc gcgccatgcg gcgttgctcc tgcttgcggg cacgagcccg cggcgtcgtc
    36901 gcgtcttgcc tgccacgccg agcgcggcac gggcgggcgc gtccaactgc cgtttctagg
    36961 ttgaacccag gttgaagcgc agcgaggtca tccttcatgt cgaaagcgag ttcgtgcgag
    37021 ccgtcgctga agtacgaatc gtaactacgc acaaacccga tgcgggaagc tgccgatgca
    37081 cgcccgcagg gcgctcggct accgtcgggc gatcatgccg tcatcgctgg tcgcccgtcc
    37141 cgccgaaccc tcgcgccgcc ttttgctgcg gctggcggcg gccggcgcgc tcggcctggc
    37201 tggcgcggcg gcggcacagc ggccgacgcc cgagcgcacc agcgtgacgc tggcggtggg
    37261 cggcaagacc tcgctgccct gtctgccgct gaccatcgcc gagcagctgg gctattttcg
    37321 tgacgagggc ctgcaggtcg aactgcagga tcaccccggc ggcggcctgg cgcagcaggc
    37381 gctgctgcag gggcgtgccg acgtcgcggc cggggggttc gagcacccgg tgctgctgcg
    37441 cccgcgcggc acccactgcc gcgccttcgt gctgctgggg cgcgcgccgc aactggtgtt
    37501 cggtgtcggc acgcgggcgc tgcccgagtt tcgttccctg gcccagctca gggggcggcg
    37561 cgtgggcgtc acctcggccg actcgtcctc gcactggttc gcccagcgcc tgctggcgcg
    37621 cgccgggctg gcgccgaccg atgtcgaata cgtcggcgtc ggcaccgcca tcacgccggt
    37681 ggcggcgctg cgcgaagggc gcatcgacgc cctggccgac atcgaccccg ccatcagcct
    37741 gctggagtac cgcgccgaca tccgcgtggt ggccgacacg cgctcgctgc gcggcacgca
    37801 cgagctgtac ggcggcccca tgccgggcgc ctgcctgtac gcgcgcgacg ccttcgtgct
    37861 gcgctacccg cagaccgtgc aggccctgac caacgcggtg gtgcgggcgc tcaagtggct
    37921 gcacaccgcc gggcccagcg acatcgtgcg caccgtgccc gaaagctaca tgcacggcga
    37981 ccgctcggtc tacctgtcgg cgctcgacaa ggcgcgcgag gccctgtcgc cggacggcat
    38041 ggccagcgac gacagcgtgg ccaccgcgca ccgcatcgtg gcgcagcagc tgcaggccac
    38101 cgacggcctg cgcgccaccg cagtgggggc gagccacacc aacgacttcg cgcggcgcgc
    38161 gcggcagcgc tttctggtgt cgtgacgcgg cgcggccacg ccgccgcggc gtcggctcag
    38221 cggcgcgcct tccggccgcc cttgccgggc gccgccgcag cggccttgcc accgcgcgcg
    38281 gcgcgcgtgg gcgcggcggc ctgcttggcg ccacccgccg ccctggcctt ggtcttgccc
    38341 ggcgaccggt cggcacgtgc cgaccgcggg ctgtcggcgc tttcgcgcgc ggcggcgctg
    38401 ctggcgcgca ccggcaggaa catcaccacc gactggcccg gcttcaggcg ggcgccagcc
    38461 tgggtgccgt tccagccggc gacggtggcg gccggcaggt cgtagcgcgc ggccacgctg
    38521 gcgatggtgt cacccttgcg tgcgcgcacc cgggtgcggc gcagcacgat ctcgggcgca
    38581 tacgccagct gggcgttgtt caccacatgg gaggcgactg cggtggcgct gccgttggcg
    38641 cgccgcacca gcacggtgga gccgctcttg atcaccacgc cgtgcgggag gttgttgatc
    38701 tcgcgcaggt cggactcgtc catgccgaag cggctggcca cctcgcgcga gggcaggttc
    38761 tgcggcgcca cccaggcggt ccacgaactc agcgtgcccg gatcggcctt ggccagcgcc
    38821 tgctgaaaca gggcggcgtt gttccagggc agcagcacct gcggcgtgcc ggcggcgaag
    38881 atcaccggct tgcgctgcga cgggttgagc gccttgaagt cctccagccg cacctgggcc
    38941 aggcgggcgg cgacgtcgac gtcgatgtcg tgcttgatgt ccaccgtgtc gaagaagggg
    39001 tggttctcga tcagcggcag caccgcgccg tggcgctgcg gctgggcgat gatgttcttg
    39061 accgcctgca gcttgggcac gtagttgcgc gtctcggccg gcatcttcag gtccaggtag
    39121 ccggtgccca ggccggcggc ctggttgcgt tcgatggcgc gcttgacgtt gccctggccc
    39181 cagttgtagg cggccagcgc caggtgccag tcgccgaaca tgtcgtgcag cttctgcagg
    39241 tagtccagcg ccgcccgggt ggagtcgagc acgtcgcgcc ggtcgtcgcg caggatgttc
    39301 tgcgtcagct ggaagtcgcg cccggtgccg ggcatgaact gccacatgcc ggccgccttg
    39361 gcgctggaca ccgcctgcgg attgaaggcc gattcgatgt agggcagcag cgccagctcg
    39421 gtcggcatgc cgcgcagctc cagctcctcg accacgtgga acaggtacag gcgcgagcgc
    39481 tcgaccatgc gctcgatgta gtccgggcgc gtgctgtacc actgctcgtg gcgctgcacc
    39541 agctcgtcct gcaggtccgg catggcgtag ccgcggcgga tgcgctccca cagatcggcc
    39601 ggcgtttcca gcgccgccac gtggttgccg cgcagctcgg tgatgcgcac cggcggcagc
    39661 gcgtccggca tgcgctgggc gccgcgcggc tcggcgcgcg tgggcgtggc ggtgctgacg
    39721 tcgcgttccg gcgtggcggg gccggggggg gcggcgcaac cggccagcca caggccggcc
    39781 agcagcgtca gagagagggt ccacttcatc ggaattcgtt cttccattga cgcagggcgg
    39841 cgaacaccgc caccgcgtcg tttgccgagg ggctgtgcgc ccgcaccgcc cgggtcaccg
    39901 ccgcttcgcg gctgcgcagg aatgggttga tcgcacgctc cagcccgatc gtcgagggca
    39961 gggtcggccg gccgtcggcg cgccgctgca ggcactggcg ctcatggtcg gccagggcgg
    40021 tgttgtccgg ctccacggtg cgcgcaaagc gcaggttgcc cagcgtgtat tcgtgtgcgg
    40081 cgcacacccg ggtggtcggc ggcagcgccg ccagcgcatc cagcgaggcc agcagctgcg
    40141 ccggcgtgcc ttcgaaaatg cgcccgcagc cacccgaaaa cagcgtgtcg ccgcagaaca
    40201 gcagcggcgc gccatccacg tcgcgggcaa agaaggcaat gtggccggcc gtgtggcctg
    40261 gcacgtccag cacctcgaag tccagcccca gcacgcgcag ccggtcaccg ccggccagcg
    40321 gggtgaacgg ctcggggatg cgctcgcgcg ccgggccgta cacggtggcg ccgctggctt
    40381 cgcgcagcgc ggtcacgccg ccggtgtgat cgccgtggtg gtgcgtgact agaatgcagg
    40441 ccagccgcag gccgagccgc ctgagcgccg cctgcaccgg cgctgcgtcg ccggggtcca
    40501 ccaccagggc ttgctggcca tcgtgcaaca accagaggta gttgtcctcg aaggcgggga
    40561 gggcgatcag atccatgtgg gggcggatta tagaaaccga tggggcggcc ttggcgcgca
    40621 gattcgagcc tcaccacgta cgccggagca tcccgtgacc ggcctgcacc actggttcca
    40681 gaccccgccc ggccagtacc tgctggcatg ggagcgcgcc cagttcgacg ccgcgctggc
    40741 cgacgtgttc ggctaccacg cgctgcaact gggcctggcc gacatcgacg ccctggccgc
    40801 caaccgcatg ccgcaccgct ggctggcact gaccgcgttg ccgccagctg cggacgaggc
    40861 ggcgcccagc gccgggcgct gggccctggt gagcgacagc accgcgctgc cctttgccga
    40921 agccagcctg gacctggtgg tgctgcccca cacgctggag ctcagccccg acccgcacgc
    40981 caccctgcgc gaagtgcagc gcgtgctggt gcacgaaggc aaggtggcga ttgccggcct
    41041 caacccgatc agcctgtggg gcctgcgcca gtggcgcagc cacctgtacc gcggcctcgg
    41101 cggcgggcag ccgtttctgc cccaagcggg cgaattcatc ggccactggc gactgcgcga
    41161 ctggctgcag ttgctgcagt tcgagctgga atcggtcagc ttcggctgct ggcgcccggc
    41221 cgtgcgcagc ccccgccggc tggcgcgcta cgcctggatg gaccgcctgg gcgcgcgctg
    41281 gtggccggtg ttcggcgccg cctatgtcat gctggcggtc aagcgcacgc atggcgcgcg
    41341 gctgatcggc gcgcgctgga aggcggcgcc cgtggccgcc acggcgccgg cgacggtggc
    41401 ccaccgccgc gccgacgccc ccaccctgac ggaaaccgaa cttgaaccac gttgagatct
    41461 acaccgacgg cgcctgcaag ggcaaccccg gccccggcgg ctggggcgtg ctgctgcgct
    41521 cgcccggcca cgaaaaagag ctgtacggcg gcgagccgca gaccaccaac aaccgcatgg
    41581 aactgacggc ggtgatcgag ggcctgcgcg cgctcaagcg cccgtgcgaa gtcgatctgt
    41641 acctggacag ccagtacgtg cgccagggca tcaccgaatg gatcgccggc tggaaggcca
    41701 ggggctggcg caccgccagc aagcagccgg tcaaaaacgt cgatctgtgg cagcagctcg
    41761 atgccctggt cggcggcgcc ggccatcgga tccggtggca ctgggtgcgc ggccacaccg
    41821 gccacgaagg caacgaacgc gccgacgcgc tggccaaccg tggcgtgccg cgcggctgaa
    41881 cggtgcgtgc aggcgccggt cgcagcgcca ccgcgcccac ctcggcgcgg ccggcgcgca
    41941 accggtaaat tacgcgcctg attcacactt gctcacagct tcgatgccag cacctttgca
    42001 cccccccatg ccagccgccg ccggagcccg ccgtggctag gcgcccgtgg gtatcgatgg
    42061 tcgccgtcgg ggcgctgctg gcgctgtcgg ccggcgcctg gtggtggcag cgcgcgggcg
    42121 gcagcgcgcc cgcgggtggc ccggtgccgg ccgccagtgc cgcgcgcggg ccgggcaacg
    42181 gcgcggccgg cggggccggt ggcggggcgc cgccggtggt cgagctggca acggcgcggg
    42241 cgatggtgct gagcgactcg gccagcgccg tcggcagcct gcgtgcgcgc cagagcgtgg
    42301 tgctgcggcc cgaggtcgcc ggacgcgtga cgcagctcgg ctttcgcgac ggcgagcgcg
    42361 tgcggcgcgg tcagttgctg gtgcagctcg acgaccggct ggcgctggcg caggtgcagc
    42421 aggcgcgggc cgaactgggc atcgccgaat ccaaccaccg gcgcaacgcc gatctggcgg
    42481 ggcagggctt catcagcgcg cgcgcggtgg acgaaagcca ggccgcggtg gcggtggcgc
    42541 gcgccaagct ggagctggcc caggccagcg ccgcgcggct gaagatcctg gcgccgttcg
    42601 gcgcgctggc cggcatcggc aacgtgcacg tgggcgacgt gctgcaggcg ggcgccgaca
    42661 tcgtgcacct ggacgacctg gacgccatgc tgctggactt tcgcctgccc gagcgctggc
    42721 aggggcgggt gcggcgcggc cagatcagcc acgtgacgct ggacgcgctg cccggtcacg
    42781 ccttcgaggc cgtgatccag gccatcgacc cgcagatcga cgcccagggc cgcagcgtgg
    42841 cggtgcgcgc ctgcatcgac aaccgccagg cgctgctgcg ccccggcatg ttcgcgcgcg
    42901 tggacacccg ctttggcgag cgcagcggcg ccgtcatgct ggccgaggag gcggtgctgt
    42961 cgcagggcgg caacctgtac gtgtaccggc tggccgcggg gccggcggcg ggcgcgtcgg
    43021 cgcccgaagg caccgccgag gtcgagcgcg tgcccgtcac cctgggggcg cgccaggccg
    43081 gcctggtcga ggcggtaagt ggcgtggccg ccggcgaacg catcgtggtg gccggccagc
    43141 agcgcctgaa cgcggcgcgc acgcgcgtgc gcctgacgcg cgcggcagac gcggccgccg
    43201 gcgccgacgg cccggcgccg gcggccagcg ccgccgcggc ggccccggcg cccgccgcac
    43261 cgctgcagtg gcaccgcgcc gggtccggcg gcaacgcctg cgcggcggcg gcgcgcggct
    43321 gagggggcat gcccatgccg acggcgccct tcgatactac gaaaatgata gctgctggcg
    43381 cttgctgcat aagcgccata gcctgttttt gcttaaaacc cagcgtgccg agcagtgccc
    43441 atgcaactgg ctgaagtctc gatccgccgc ccggtgctgg ccaccgtgct gtcgctgctg
    43501 atcgtgctga tcggcctggt cagctacagc cggctggcgg tgcgcgaata ccccaagatc
    43561 gacgagccgg tggtcaccgt cagcgtgcgc tacgtcggcg cctcgg
//
LOCUS       DAOOOG010000005        43475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_632_length_43475_cov_25.596614, whole genome shotgun sequence.
ACCESSION   DAOOOG010000005 DAOOOG010000000
VERSION     DAOOOG010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 43475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43475
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_632_length_43475_cov_25.596614"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="PK242_00955"
     CDS             complement(<1..1334)
                     /locus_tag="PK242_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HOZ92627.1"
                     /translation="MATDIEKAPEALQQLVAETDTGGRKPSGLTARLMFGLALAWAVF
                     QFWYASPLPFALGWGILNDTEARAIHLAFSLALAFLAWPAFRSSPRRHVPLVDWGLAI
                     AGAFAGAYLMLFYRELATRPGQPSVMDIVTASAGLLLLLEATRRAVGWPMAALAALFL
                     AYCVAGPWMPEVLAHKGASLSRLLSHMWLTTEGVYGIALGVSASTIFIYVLFGALLDR
                     AGGGNYMMQVSFAALGHLRGGPAKVAVVSSALNGMISGSSVSNVVSGGIFTIPMMKKA
                     GYGGVKAGAIETMSSVNGQIMPPVMGAAAFLMVEYVGIPYSDVVRHAFLPASLSYLGL
                     LYIVHLEALKLGIQPAAAAAPRPWRARLMRTLIGSSGALAVVGALYHLLQFFKSALGA
                     AAPWAVGGTLLALYLVALRQAAVSPDLPEDIDVHHPQRLETWPTVRAGLHYL"
     gene            complement(1501..2481)
                     /locus_tag="PK242_00960"
     CDS             complement(1501..2481)
                     /locus_tag="PK242_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOZ92628.1"
                     /translation="MSLRPVLKTLAAAAALSTAALAAPASAQQKFVTIGTGGVTGVYY
                     AAGGAICRLVNKDRAKHGIRCSVESTGGSVFNVNTIKAGELDLGFAQSDVQYNATKGL
                     SQFKDGGAVSDLRAVFAVHPEPFTVVARKEAGITKFEDFKGKRFNVGNPGSGTRASME
                     ELLAAMGWKLSDFSLASELKADEHGPAVCDGKIDGFFYGVGHPSANIQDPTTSCGAKL
                     VPLTGPVVDKLVADKPYYAKVSIPAGLYPNNPQATPTYGVLATVVASSKTPADTIYAV
                     TKAVFDNFDEFKKLHPALAHLKPEDMVRNGLSAPLHDGAARYYKEKGWIK"
     gene            complement(2516..3850)
                     /locus_tag="PK242_00965"
     CDS             complement(2516..3850)
                     /locus_tag="PK242_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOZ92629.1"
                     /translation="MSTTHVMHRHLHHTPPVAVGGQGVWLHDAAGRSYLDASGGAAVS
                     CLGHGHPDVLAAMHRQIDQLAYAHTSFFTTEVAEQLAEQLVRTAPAGMSHAYFVSGGS
                     EAVEAALKMARQYYLEIGQPERSHFIARRQSYHGNTLGALAVGGNEWRRRQFAPLLVD
                     VTHVSPCYPYRDQQPGETPEQYGQRLARELDEAICRVGPGKVLGFVAETVGGATAGVL
                     VPVPGYFKAVREVCDRHGVLLILDEVMCGMGRTGTLHACQQDGVVPDLMTVAKGLGGG
                     YQPIGAVLAQQRIVAAMSAGSGFFQHGHTYLGHAVACAAALAVQQVIERDGLLQKVQR
                     GAQVLQALLAERFAHHPHVGEVRGRGYFWGIELVAERAGKTPFDPARRLHARVRSEAF
                     ARGLLVYPMGGTVDGRYGDHILLAPPFIATDDELALIAERLAAAVDAAIAQG"
     gene            complement(3888..4661)
                     /locus_tag="PK242_00970"
     CDS             complement(3888..4661)
                     /locus_tag="PK242_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HOZ92630.1"
                     /translation="MSLKGKTALVTGSTSGIGLGIAIELARQGAHIVMNGFGDVDAPR
                     RQIEACGVQTAYHGADMSKPAEIEAMMAFAAEKFGGVDILVNNAGIQHVSPIEGFPTE
                     RWDAIIAINLSSAFHTTRLAIPHMKARNWGRIINIASVHGLVASKDKSAYVAAKHGIV
                     GLTKVTALELATTGVTANAICPGWVLTPLVQKQIDARAAAEKISTEQATRELLGEKEP
                     SLQFTTPEQLGAMAVFFCSAAAANVRGQAWANDGGWTAQ"
     gene            complement(4843..5484)
                     /locus_tag="PK242_00975"
     CDS             complement(4843..5484)
                     /locus_tag="PK242_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOZ92631.1"
                     /translation="MAWTQDQMAARAAQELQDGFYVNLGIGIPTLVANHVPADKEVWL
                     QSENGMLGIGPFPTADEVDADLINAGKQTVTTIKGSSIFGSHDSFAMIRGGKINLSIL
                     GAMQVTDKGDLANWMIPGKMVKGMGGAMDLVAGVARVIVLMEHVAKKKDGSTDMKILP
                     QCTLPLTGVGVVDRIITDLGVFDVTDRGLKVVELAPDVSFDEVQGKTGVPLLQ"
     gene            complement(5501..6202)
                     /locus_tag="PK242_00980"
     CDS             complement(5501..6202)
                     /locus_tag="PK242_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOZ92632.1"
                     /translation="MNKIYPSAADALKGVVHDGQQLAVGGFGLCGIPEALIDALRDSG
                     VKNLTVISNNAGVDGFGLGKLLETRQIKKMISSYVGENKEFERQFLAGELELEFTPQG
                     TLAEKLRAGGAGIPAFFTKTGVGTLVAEGKELREFDGQTYVMERSLVADVALVKADVA
                     DRSGNLRFNLTARNFNPAAAMSGKVCIVEVERIVANGELAPDDIHLPGIYVHRIVHNP
                     TPEKRIEKLTLREHK"
     gene            6292..6888
                     /locus_tag="PK242_00985"
     CDS             6292..6888
                     /locus_tag="PK242_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOZ92633.1"
                     /translation="MAIPVPAAGDPALAPPDYRLAFQLAPVGLILSRQRHIVDCNDRV
                     CDIFGAEPAQLIGQSFRILYPSADEYERFGARIAPILNRDGLYADNRVMQRVGGPQAG
                     EAFWCHVTGRAIDRRAPHAAGIWSFEDLSAQRPMGAVLTAREREVAAQLTCGLTSKEI
                     GRALGLSHRTVEVHRAKLMRKYRAGTTAELVQKLMGGL"
     gene            complement(6959..7360)
                     /gene="pcaC"
                     /locus_tag="PK242_00990"
     CDS             complement(6959..7360)
                     /gene="pcaC"
                     /locus_tag="PK242_00990"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HOZ92634.1"
                     /translation="MTLPDDPAFQHGLKKRRQVMGDDFVDRAFASATPFTEPMQHAIT
                     RNAWGDVWQRPGLDLKTRSLVTVAMLTALGRQHELKGHVRGALRNGATVAELQEVLLH
                     ASIYCGVPCAVEAFRSAAEVVDGGPGQSDKK"
     gene            7430..8023
                     /locus_tag="PK242_00995"
     CDS             7430..8023
                     /locus_tag="PK242_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428684.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HOZ92635.1"
                     /translation="MSRYPLPSLDSLPEDIRARILEVQAKAGFVPNVFLALARRPAEW
                     RAFFAYHDALMEPESAGRSSRLSKGDREMIVTATSAANGCLYCVVAHGALLRIYEKKP
                     LVADQVATNHRKADISARQRAMLDFAMKVSQHPRELGEEDFAPLHAHGFDDEDIWDIA
                     AIAAFFGLSNRMASFSNMTPNAEFYLMGRVPREKKPS"
     gene            8214..11582
                     /gene="cas9"
                     /locus_tag="PK242_01000"
     CDS             8214..11582
                     /gene="cas9"
                     /locus_tag="PK242_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655176.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOZ92636.1"
                     /translation="MTHYVFGLDIGIASVGWAVLSEQRIIDLGVRCFDKAETAKEGEP
                     LNLTRRQARLLRRRLHRRAWRLTQLARLLKREGLIADAKLFAKAPSFPDNAWQLRLQG
                     VDRLLTPLEWARVIYHLCKHRGFHWVSKAEEAKADSDKEGGRVKQGLKHTGALMAEKG
                     YRSAAEMVLAEFPGAQRNKGGQYDKALSRVLLGEEMALLFQRQRTLGSAHASERFEAL
                     ILGSGDRRSGLFWRQKPALSGADLLKMLGKCTFERNEFRAPKASFSAERHVWLTRLNN
                     LRVIIDGCSRALSDAERAVVLSLPYQTEAFKYKSLKNALVKAGLWSEEVRFGGLAYPS
                     RAQAEGENAKDPEDQALVKMPAWHELRKAFKAAGQESLWKRISTPALEGEPALLDQIA
                     TVLSVYKDGAEVVQQLRGLDLPDPDVSITVLERISFDKFSSLSLKALRRIVPLMEAGM
                     RYDEAVAQIPEYGHHSQLVKPGAEKHLYLPPFYEAQREYRSKNDRVGSMKFRDDADIP
                     RNPVVLRALNQARKVVNALIREYGSPMAVHIEMARDLSRPLEERNKVRRAQDEFRERN
                     DKAREAFESENGYRPKGAVFEKWLLYREQLGQCAYSHQPLDIGRVLDDPNYAQVDHAL
                     PYSRSYDDSKNNKVLVLTQENQNKGNRTPYEYLTALDGGQEGERWRAFVAWVQGNKSY
                     RMAKRGRLLRKNFGGDEARGFIDRNLNDTRYICRFFKNYVEEHLQLAPRADGDLNRRC
                     VVVSGQLTGFLRARWGLAKVRSDSDRHHALDAAVVAACSHGMVKALADYSRRKEIEFI
                     KEGFPDPETGEILNPTAFDHAKRFFPEPWPHFRHELQARLFTDDLAALRADMQRLGTY
                     PDDALVRLRTLFVSRAPQRRNGGAVHKETIYAQPDSLKARGGIIEKVLLKDLKLSDLD
                     NLVEPHRNERLYSAIRERLQAHAGKADKAFGPDNPLHKPDKSGNPTGPVVRSVKLVRA
                     KQSGISIRGGLAKNDSMLRVDVFTKAGKFHLVPVYVHHRVTGLPDRAIVAHKDENDWP
                     HMDETYQFLHSIYPNDLIRITQKGASFSGYYAGCDRGSGNVNLWAHDRAIAVGKDGLI
                     RGIGVKTALSLEKFHVDVLGRIYPAPPEVRCGLA"
     gene            11569..12477
                     /gene="cas1"
                     /locus_tag="PK242_01005"
     CDS             11569..12477
                     /gene="cas1"
                     /locus_tag="PK242_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOZ92637.1"
                     /translation="MVWRSVVINQPARLKREHFALVVEQEQSARVPFEDIAVIVLNHR
                     EITLTHPVLSACADYGIGLYSTGDNHQPNGIFMPFLPHSRATRMQRLQLQLDKPSTKR
                     AWASIVRAKIANQARCMELLGRGDAERLHSYVRRVRSGDAGNLEAQASSYYFPHVFGR
                     NFHRSQPGWVNGALDYGYAVMRGACARALVQHGMLPSLGLFHSSEQNAFNLADDLIEP
                     YRPVVDLHVAQQPTRSDETELRPADKVALIGLLNVDVSMPRGRMSVLASIEQAAEALA
                     RLYDGGSEQVLELPGLIGLAQHAFEM"
     gene            12513..12821
                     /gene="cas2"
                     /locus_tag="PK242_01010"
     CDS             12513..12821
                     /gene="cas2"
                     /locus_tag="PK242_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOZ92638.1"
                     /translation="MRMLVFFDLPVVTKAERRAYTLFRRFLLNDGYDMIQFSVYGRIL
                     NGSDAVDKHMKRLLANLPPEGSVRVLTVTEKQFASMKLLVGLPLFQEKKVNAAQIALF
                     "
     repeat_region   12886..13793
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12886..12922
                     /rpt_unit_seq="gagtgtagctatccggggtgagagagggagctacaac"
     gene            14044..14118
                     /locus_tag="PK242_01015"
     tRNA            14044..14118
                     /locus_tag="PK242_01015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14077..14079,aa:Arg,seq:cct)
     gene            14155..15369
                     /gene="gspF"
                     /locus_tag="PK242_01020"
     CDS             14155..15369
                     /gene="gspF"
                     /locus_tag="PK242_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HOZ92639.1"
                     /translation="MPAYTFEALQADGKSRQGVIEADSARAARALLREQALVPLTVEP
                     VTTGGSVLTRPLGGARAFNASQLAIWTRQLAGLIGAGLPLERALTALVDEAEHERQRH
                     LLAALRAEVNAGAPLARAMAQHPRDFSPVYTAVIAAGEQGGHLGLVLERLADDLEERQ
                     TLKAKLIGAALYPAIVTVVAIVIVVFLVSYVVPQVASVFAGSKQALPTLTVLMMALSQ
                     FVRAWGGWLLLALGLIAVGARLALQNDGLRQKFDAAWLRLPLIGRLARGYNAARFGAT
                     LAMLAGAGVPILRALQAAGETLHNRAMRADAEDALVMVREGAPLAAALAQKPRFPGLL
                     AMFARMGEQTGQLPAMLQRASAQLSAEVQRRALQLATVLEPLLIVVMGVVVLLIVLAV
                     MLPIIQLNQWVR"
     gene            15570..16514
                     /locus_tag="PK242_01025"
     CDS             15570..16514
                     /locus_tag="PK242_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOZ92640.1"
                     /translation="MTIALDDKLVVAISSRALFDLEEENRLFESGDPEGYMRVQLQRL
                     DTPARPGIAHAMVRKLLRFNEDGVERVEVVILSRNDPVSGMRIFNSGAAQALRLERGV
                     FTQGRAPFGYLRPLRADIFLSANADDVREALAAGFPAAHVMVDAAPASDRYPDEVRIA
                     FDGDAVLFSDQAEQVFQKKGLAAFQRHEQRKADEPLPDGPFKPFLVALHRLQQAGVPH
                     MRIRTALVTARGAPAHRRAIQTLMRWNIRVDEAMFLGGLPKGEFLREFEPDFFFDDQS
                     GHIAHAARHVPAGHVPEGVTNATPAAETSRRRRPSKRR"
     gene            16511..16906
                     /locus_tag="PK242_01030"
     CDS             16511..16906
                     /locus_tag="PK242_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HOZ92641.1"
                     /translation="MKPSFWHLPLLLLAVWASPAPAQTAAAATTAPGAARVSKGEAQA
                     LKIFRMLDADGDGRISRQEAQVGIRLKPSLAEDFRRADLNGDGHLTEQEIRSVAERRR
                     AERLERRRKEQALAARPATEPRSGSAVRP"
     gene            complement(16934..18244)
                     /locus_tag="PK242_01035"
     CDS             complement(16934..18244)
                     /locus_tag="PK242_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HOZ92642.1"
                     /translation="MSDTDTLLPVSAWPYTQILMRLALGLALGLLIGLERERRGKEAG
                     LRTFGFIGLLGTMGGALGMGYGLATLALTGLLVLILNVSSLRANQGTELTTSAAMLVT
                     GMAGIMCGRGHTIAPAAVMVIATALLAWKERLADFSMGVSEGEIRSAILLAVLAIVIY
                     PALPQGTVGPWGLIEPRAAWVAVILIAGIGFFNYVLWKMYGTRGTELSGFFGGLINSN
                     FTVIELVERVREVGVAYVGTAYRGIMLAMVAMIARNATLLLILAPEALISAFSAFLLM
                     MGASALLVGVSLRSGRRSAAGVAPEIHLDMPFSLPQALKYGAIFLLLHALGALTQRQF
                     GDAGFYFVSIVGGLLSSASSVAAAASLAAQGGLPPGTAGVGAVLASFTSIVFSLSFVL
                     RSRSGALIRQLALSMLLIAAAGLVGLLAGQFMQPWVGALLSRME"
     gene            18520..20340
                     /locus_tag="PK242_01040"
     CDS             18520..20340
                     /locus_tag="PK242_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HOZ92643.1"
                     /translation="MPMPQRLAQFQQFLRQVWRLSAPYFSASDERWRARGLLTAIVVL
                     NLGAVYMLVQINEWNRVFYDALQNKDAAVFWTQLGRFTLLAMAFVVIAVYRFYLTQLL
                     QLRWRAWMTRDLMGRWLQGQAFYRMELARYAGAAVPTTGATGLPHPGQREPDNPDQRL
                     QEDVNLFTAQTVGLSMGLLNAVVTLASFVGILWGLSGAFSFTLGERTIELPGFMVWLA
                     LLYCIAGSVLTHYIGRPLIGLNFEQQKREADFRHHLVRVREYSEAIALDRGERVEQRN
                     LDTRFTHLLANYLRLIGKQKQLVGFTNLFGQAAVVFPFIVAAPRFFSGAIQLGELMQI
                     ASAFDRVQGALSWFVENYDSLASWRATTERLTSFEAAISKQNTAAEPTQQAPAATDSG
                     VIEAHGLNLRLPTGASLLAATELRVAPGEHTLVQGPSGSGKSTLFRALAGIWPFAQGQ
                     VALPARTMFIPQRPYVPDGALRDALAYPEPASRYSDAQLQDALRDALLPALAARLDER
                     DNWTQKLSGGELQRLAIARVLLRQPAWVLADEATSALDVAAEQQLYARLKALTERTGG
                     ALVSIAHRPGVAAFHDRYWRFVPQTEGSAARYRIESAALA"
     gene            complement(20421..21053)
                     /locus_tag="PK242_01045"
     CDS             complement(20421..21053)
                     /locus_tag="PK242_01045"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOZ92644.1"
                     /translation="MTAHAHRTTRCHPRSVPRRPAGPPWRHLRRAGGTALLLSVLWGG
                     AGAVQAQVTEGVDWSLSAAAAADAGVRKLGLIAGWTQAAPLWQGTQWRLRLRHEIELA
                     AWHVPRARDLVELGYSPVLRLERPLDGAHATLFLEGSIGARLLSRTRVAPQRSLSTAF
                     QFSDMLGVGVQWGTQARSTLGLRYQHVSNLGIKEPNPGMDFVQVYYTHRF"
     gene            complement(21149..21769)
                     /locus_tag="PK242_01050"
     CDS             complement(21149..21769)
                     /locus_tag="PK242_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOZ92645.1"
                     /translation="MTDLVPLTAAVRPRGSDELAQALIHGRHTVLPRRLGLPGPDAAQ
                     TERILGAAASAPDHHTLLPWRFILVPDAARPRLAEAFAAALVQRDPQATDEQIGQARE
                     KAFRAPLLMLAVVRLRDDDADIPPHERIVSAGCAIQNLLLMAHALGFGAALTSGKALA
                     SAPLRTLFGLGEAEQALCFVSVGTVLKARPARARPLPAQYVSTLAP"
     gene            complement(21971..25594)
                     /locus_tag="PK242_01055"
     CDS             complement(21971..25594)
                     /locus_tag="PK242_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HOZ92646.1"
                     /translation="MKRDSLPGAQHALSAQPISRDVLAEKYFKPGETGLDQLYGRVAR
                     ALASVEKPELRAEWEQKFLANLHAGAIGAGRIMSAAGTDIQATLINCFVQPVGDAIQG
                     RDADGYPGIYEALREAAETMRRGGGVGYDFSRIRPKGAFVKGTHSFASGPCSYMNVFD
                     QSCSTVESAGSRRGAQMGVLRIDHPDVLEFITAKRTPGRWNNFNVSVGVSDAFMQAVI
                     DGSDWELAHKAEPGDKVKAKGAYQRADGLWVYRKVPARELWDTIMKSAYDFAEPGILF
                     LDQIGRDNNLNYTEQIEATNPCVTADTWVMTEDGPAQVADLMGRPFNAIVDGAAHPLL
                     SEGFFATGHKPVVHLQTREGHSLRLTADHRVRRVTRKTRYVIESAWEPAGQLRPGDEI
                     MLQQHRTLQGWDGAGTEAEGYLLGLLIGDGVLKEDKAVISVWAPELRQVAQGAPAHAL
                     SGADGIIAAAEAAARTLPHRADFGGFQRAVRGRAERRMASGALRHLALSHGLTPGRKS
                     VTPSMEKTSSAFHIGALRGLFDTDGSVQGAQDKGVSVRLAQSDLGTLQAAQRMLLRLG
                     IASTIYPERRPTGVRALPDGRGGLDCYRTAAQHELVVSGDNLALFADRIGFADTDKAA
                     RLTGALAQYQRQLNRERFTATVSAVTPDGSADVYDVTVADVHAFDANGLYVHNCGEQP
                     LPPYGCCDLGPIILTNFVRHPFGAGGEPSFDFDAFEQVVAVQVRALDNVLDVTFWPLD
                     QQRAESASKRRIGVGFTGLGNALSMLKLRYDREPGRQMAAEIGRRMRDAAYRASVELA
                     KEKGPFPKFQADGYLAQGTFASRLPEDIQAAIRQHGIRNSHLLSIAPTGTVSLAFADN
                     ASNGIEPAFSWGYKRNKREHDGSTSSYMVEDHAFRLYRSLIDPGATPEQPETLPEYFV
                     NALEMTAHEHVAMMQAVQPYVDTSISKTVNIPEDYPYEDFKDLYRQAWQAGLKGLATY
                     RPNSILGAVLETTPAKKEEPVAQAPAPLPVTPYDPMRTVIEKRPAGGLPAVAEKIEYW
                     TSDGQQRLYLIVTFLPVPSADGKGTIERAIEFFMPVGQSGESQQWITATMRMLSLAAR
                     GGFLDRALSDMRKVTWDRGPVRLGTYQKADGTHVPRWHDSEVAAIGYAIENLIVRRAQ
                     AAQASLFEPDELLQAEPEASASAAVPGVMAGKKCPECGAHAMIRKDGCDYCTQCGYVG
                     SCG"
     gene            complement(25894..27426)
                     /locus_tag="PK242_01060"
     CDS             complement(25894..27426)
                     /locus_tag="PK242_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOZ92647.1"
                     /translation="MSAEPAFRAVADLIREHARRDPAHPALIDDRQQLSYGELDALMD
                     RVAAALQRDGVSPGEAIVVCGLNAVPYAALYLGALRAGVVVAPLAPSSTPQALAAMVA
                     DARARVLFLDATAPAQHLPGPDRLPRVSLDGVAPGRAFADWLAPVGAQPAPVALVPEA
                     PFNIIYSSGTTGTPKGIVQSHGMRWMHVRRGAVYDYGPHGVTLLSTPLYSNTTLVVFF
                     PTLGYGGAVVLMPKFDALRYLQLAQQHRVTHTMLVPVQYQRIMALPQFGAFDLSAFRV
                     KFCTSAPFSAALKADVLARWPGGLTEFYGMTEGGGTCILDAHLHPDKLHTVGRPAQGH
                     DIRLIDEQGREVASGEAGEVVGHSPGMMTGYHNQPDKTREAEWFDAAGKRFIRTGDVG
                     RFDAEGFLTLLDRRKDMIISGGFNIYPSDLEAQLRQHPAVADVAVVGVPSERWGETPV
                     AFVVPQPGQAVEAAALMHWFNQRAGKTQRLADLHLLPELPRSPIGKVLKRDLRDGYGR
                     TG"
     gene            complement(27465..28424)
                     /locus_tag="PK242_01065"
     CDS             complement(27465..28424)
                     /locus_tag="PK242_01065"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ92648.1"
                     /translation="MKRRLLTLVVACMGAWYATAVQAQADYPNQSITMIVPYAPGGTT
                     DVLGRALAASLSRQLKQPVVVENKSGAGGTLGVVDLMRAKPDGYRLTLVPVSVFRQPY
                     VQKTPYDPIRDLTYIASFTAYDFILAVAADSPFKSVKDVVEHARGRKDAVDYGTPGKF
                     TGNQVAMALLAKAAGVSFVHVPFKGDSESTAALLGGHVKTIVSTNGVLSFMESGKVRA
                     LAIAADQRPPAFAGVPTFREAGYDVVVPSPLGLAGPKGLPSAIVQKLDAAVKAALDDP
                     DMQRVIKTYGVRTDYRDHQAYAEFARRNFEAEKSIVQSLGLND"
     gene            complement(28560..29759)
                     /locus_tag="PK242_01070"
     CDS             complement(28560..29759)
                     /locus_tag="PK242_01070"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOZ92649.1"
                     /translation="MRQAVIVSTARTPLAKSHRGEFNITPGPQLGAHAVRAAVERAGI
                     EPGLIEDLIWGCGYPEGTMGRNIGRQTALRAGLPVTVGGTVINRFCSSGLQAIAVAAG
                     RIVMDGVPAMVAGGAESISLLNPAGRGGTAHLDPWLVEHKPQLFMEMIDTADVVAARY
                     GISREDQDAFALQSQQRTAAAQAGGVFDNEIVPCKTTMAEKNKDTGEVTHKEVTVTMD
                     NCNRPTTTLAGLAGLEPVKGAGKFVTAGNASQLSDGAAACVLMEAQEAERRGLQPLGA
                     FRGLAIAGCEPDEMGIGPVFAVPRLLERTGLKVEDIDLWELNEAFASQALYCQRRLAI
                     PMERLNVNGGAISIGHPFGMTGARLAGHILLEGQRRKARGERVKWGVVTMCIGGGMGA
                     AGLFEIF"
     gene            complement(29994..32114)
                     /locus_tag="PK242_01075"
     CDS             complement(29994..32114)
                     /locus_tag="PK242_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOZ92650.1"
                     /translation="MNASQGAGPVQTRLDGDVLVVTIDNPPVNALGQAVRAGLLAAVE
                     ELERTAQARAMLIVGAGKAFIAGADIREFGKPPQPPSLPETINRMEACDKLVVCAIHG
                     PALGGGLEVAMATHYRLALPAARLGLPEVALGLLPGAGGTQRSPRLMGALPAAELMLS
                     GKHLSAKAALAAGLVDRLVEGDDPLAAGLAYVRELLAAGTAPRRTSALAVPGDKAQTL
                     AALDQLAQDTAKKTRGLFSPARIIDCVRVAVQTPFAEGAKRERELFVECLNSPQRAGL
                     VHAFFAERETAKIPEAKAAAPRPFAKLAIIGGGTMGAGITVSALDAGLSVVMIERDAE
                     SIARGQKNVEKVYDGLIAKGRMTAEAKAATMARYQPSTRYEDIADVDLVIEAVFEDIA
                     VKQAVFRELDRVCKSGCVLATNTSYLDIDAIAAVTSRPQDVIGLHFFSPANIMRLLEI
                     VVPAKVAPDVVVTAFELAKRMKKVPVRAGVCDGFIGNRILAVYRDAANAVLEDGASPY
                     EIDAAVREFGYPMGPFQVSDLAGGDIGWATRKRKAATRDPRARYVEIADRIAERGWFG
                     QKTGRGWYLYPEGARVGQQDPEVLAIVDAERQKKGIAPRAFTHEEIMRRYMAAMVNEG
                     AKVLEEGIALRPLDIDVTFLFGYGFPRHRGGPMLWADMQGLPKVLADIEEFAKEDAQF
                     WQPAALLKRLVAEGKNFASLNQRG"
     gene            32227..33147
                     /locus_tag="PK242_01080"
     CDS             32227..33147
                     /locus_tag="PK242_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOZ92651.1"
                     /translation="MDLQSLTLLVEIIDSGNLSQAARKLRMTRANVSYHLTQLEQSVG
                     AQLLRRSTRHVEPTEIGLRLYEHGRAIQNEMLAARETVTRLGQGLMGRVGISMPSGYG
                     QMVMSHWLIEFKRRYPGIVLDVVFENRVDVLRDDVDIAVRVLPEPPPHLVARELDEVR
                     YLACASSAFARTHGLPTRLEQLTTLPLITASVVGRQLSLRGYRQDERHEVLLAPTLIS
                     DHYPFLRQGILAGLGLGLVPDYVVQDVVASGAVVTALDDFRLSIFGTRMYLLHLPGRH
                     QTRAVRTCIDFLLARAAGQSAPPTPGEPPA"
     gene            complement(33309..34184)
                     /gene="htpX"
                     /locus_tag="PK242_01085"
     CDS             complement(33309..34184)
                     /gene="htpX"
                     /locus_tag="PK242_01085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HOZ92652.1"
                     /translation="MKRILLFVLTNLAVMVVVGIVTSLLGVGRYTGTNTGQLMVFSLV
                     VGFSGAIVSLLMSKSMAKMSMGVQVIAQPRNADEAWIVDTVRKFADQAGIGMPEVGIY
                     EGEPNAFATGAFKNSALVAVSTGLLRGMTREEVEAVIGHEVAHVANGDMVTMALIQGV
                     MNTFVVFASRVIGSLVDGFLRRDSESSGPGIGYYVTTIVLDILFGVLAGIIVAWFSRQ
                     REFRADRGAAQMMGQPNSMINALARLGGMHAEGAEMPKNLAAMGIAGGIGKLFSTHPP
                     MQERIAALRAFQAQP"
     gene            34393..35547
                     /gene="argE"
                     /locus_tag="PK242_01090"
     CDS             34393..35547
                     /gene="argE"
                     /locus_tag="PK242_01090"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HOZ92653.1"
                     /translation="MNTLDWLGRLVAIDTTSRNSNLGLIETVRDALTAQGLKPWLAGN
                     ADGSKANLFCTLPAADGQTQGGIILSGHTDVVPVDGQDWASDPFQLTDRGDGRLYARG
                     SCDMKGFIASSLALVPEALAAPRRKPLHLALSYDEEVGCVGAPVMLAELNRRGIRADG
                     CVVGEPTSMKPVVAHKGGYFFRCTVHGKAAHSSLTPQGCNAIEHAARLICHIRDLADA
                     WRAQGPFDAAFDVPHSTITTNLIAGGIAINTVPDACSFSYDFRHLPGHDASGIQAQVQ
                     RYVDDELLPRMRREYAGARVDIECGAAVPTLDASEQAAITQLVRALTQDQGVRKVAYG
                     TEAGQFAQAGIPAILCGPGSIEQAHKPDEYVEHAQLALCDRFLRQVLATL"
     gene            35564..37135
                     /locus_tag="PK242_01095"
     CDS             35564..37135
                     /locus_tag="PK242_01095"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ92654.1"
                     /translation="MSPTTPAACARLIAQLQHDLRASGAADVQCLETHISWLLLAGEH
                     AYKIKKPLTLDFLDFGTLAQRRAACEEELRINRRSAPELYLEVLPITGTPAAPRLGGD
                     AAQAIEWAVHMRRFPPGALLAERAARGALGPAQIDALARAVATFQAQAAQAPADSPWG
                     HAADLARLAHDNFAPLAALGAGTALAGTLQRLAHWSETEGRRLAPLMAARRAAGRVRE
                     GHGDLHLGNLLWLEGRARLFDAIEFDPALRWIDVACDIAFVFMDLHAHGLAALAWRFL
                     NAWLEHSGDFGAVARLPYDAVYRALVRAKVAALRAGQGQGGVARTAAEAEARRYVQLA
                     AQLAAPRRPWLALTVGVSGSGKSSQTQTLIERRALVRLRADVERKRLFGLAPEAASSG
                     VPGGIYDEATSERVFAHLLDEARALLQAGQPVLVDATFIRRARRAPFIALAEELGLPW
                     RLLAFDAPEQVLRQRVRERAAQGGDASEADEAVLHRQLAQRQPLTANEASHALTIDAR
                     QPVDWAAWDSALPGA"
     gene            complement(37308..38144)
                     /locus_tag="PK242_01100"
     CDS             complement(37308..38144)
                     /locus_tag="PK242_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HOZ92655.1"
                     /translation="MGIPADLSLRWPSAAALEAPWWQPYRALLAAWPPPGLAPGAPVA
                     DGLNALAAAQGDVPVRFVGQQALPEGQAYEAFIRARRQVPTRDNAHDLFNGLVWLRLP
                     RTKGRLNALEADEIARAGVGARRGPVRDACTVFDENAALLHAPDALWQALVERRWAEL
                     FGPLRPLWGQASLLVFGHAALEKLLSPYKSITAHVWRVALPFDAAGELAALDAWLAAD
                     LTPDKLARKPFVPLPLLGVPGWWPDNAQPAFYDDAEVFRPRGRARAAGGSERFFTMTN
                     DR"
     gene            38364..39518
                     /locus_tag="PK242_01105"
     CDS             38364..39518
                     /locus_tag="PK242_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOZ92656.1"
                     /translation="MPTDPRKRAFLRIAAASALGATALAGCGRKDEAGAPAAAPVAAP
                     PAAAGPLKAAWIYVGPVGNAGWSFAHDQGRLAVQAAFGDRVATSFVESVPEGADTERV
                     LRDLIGQGHTLIFGTSFGFMEAMVKLAQEHPQVRFEHATGYKTAPNLNVYDSRFYQDT
                     YLAGIIAGHMTQSGTLGYVGSVPIPEVLRNINAFTLGAQSVKPQIRTRVVWINSWYDP
                     PKETEAAQSLINAGADVLLQNTDSPAVIQAADKAGKLAFGWDSDMSAHGGAAHLASCI
                     ADWSHYYKKAVQAALDGSWKGGQVTRWGVPEGQNDLIKLSEKIPAEVRAQVEAIKAKM
                     KDGSFDVFTGPVVDVAGKERLAAGQRADDAWMGAIDFFVKGVEGQIPASK"
     gene            39576..40577
                     /locus_tag="PK242_01110"
     CDS             39576..40577
                     /locus_tag="PK242_01110"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HOZ92657.1"
                     /translation="MNLERSLWHPVALGHVVGSQPRAVTLLGEDLVLWRDGNGHAHAW
                     MDQCPHRGARLSLGRVHGGQLECPYHGWRFDGGGRCTHVPSVPDFQPGPRHGARTFDT
                     REAYGMLWVRLAAPEGEPQPIHQLPVFAAEAEPRLRKTTSGPYLVAASAPRIIENFLD
                     MAHFGYVHEGWLGSRERPEIPPYEVQATPHGVRATGCLAWQPRSSIHAASGAQVEYTY
                     EVTAPYMAVLTKVPEAGSTAIEGMNESIAMFVCPVTEESSVAWTRMAMNDFDSPDARL
                     IDFQNTIFGQDQPVLESQRPKRLPLAPAAEAHCAADRMSAAYRRYLRESGITFGVIP"
     gene            40698..41753
                     /locus_tag="PK242_01115"
     CDS             40698..41753
                     /locus_tag="PK242_01115"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOZ92658.1"
                     /translation="MSWAPPPIAAARLPALLAAAPKAELHIHIEGSLEPEMIFALAAR
                     HGIALPYADVQALRRAYAFGNLQQFLDLYYQGADVLRDEEDFYQLGRAYLARAATENI
                     VHAEIFFDPQTHTARGVPMGAVMRGLRRASDDGRRELGIGSALILCFLRHLSEREAFE
                     TLEAALPWRDDFIGVGLDSSELGHPPEKFARVFARCRELGLHVVAHAGEEGPPEYIWQ
                     ALDILKAERIDHGVQAVHDAALMRRLAQRRIPLTVCPLSNQKLQVFPDLAHHSLPQLL
                     AAGLCATVNSDDPAYFGGYVNANFQAVFAATGLDSAAAYQLAANSFEASFADAALKQR
                     WLDKLQAAFERAHDAGS"
     gene            41826..42899
                     /locus_tag="PK242_01120"
     CDS             41826..42899
                     /locus_tag="PK242_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOZ92659.1"
                     /translation="MYKNLVAALAAACCFFPLQPAQAQPPTRAAWVYVTPVLDTGWTR
                     QHELGRLAVQQALGDQVKTSYVENVAEGADAERVMRDLARQGNDIIFTTSFGYMEPAL
                     KLAQEFPRVKFEVITGVRRAPNVATANARYYEGRYLSGVAAGRMTKSNVVGYVAGFPI
                     PEILSSINAFTLGLRSVNPQATVKLVWLNEWFNPPREREAAMTLIDQGADVLAFNSAS
                     TAVMAAAEERGQLAIAYHSDMRAVAPTAQLAAVTHHWEAYDLARVRALRAGQWQSGDS
                     WGGVAAGMVRIGAFGPKVPAAVRQEVLARQKDIAAGRLQPFAGPVVDNTGRPRVPKGR
                     SASDAEILQMDYLVEGVQGSLPK"
     gene            43103..>43475
                     /gene="dcd"
                     /locus_tag="PK242_01125"
     CDS             43103..>43475
                     /gene="dcd"
                     /locus_tag="PK242_01125"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOZ92660.1"
                     /translation="MSIKSDRWIRRMAEQHGMIEPYEPGQVREVDGRRIVSYGTSSYG
                     YDIRCAPEFKIFTNIHSTVVDPKNFDEKSFVDFHGDCCIIPPNSFALARTVEYFRIPR
                     NVLTICLGKSTYARCGIIVNVT"
BASE COUNT         7010 a        15074 c        14693 g         6698 t
ORIGIN      
        1 atcaggtaat gcaggccggc gcgcaccgtg ggccaggttt ccagccgctg cgggtggtgc
       61 acgtcgatgt cttccggcag gtcggggctg acggcggcct ggcgcagcgc caccaggtac
      121 agcgccagca gcgtgccgcc caccgcccag ggtgcggcag cgcccagggc gctcttgaag
      181 aactgcagca ggtggtacag cgcccccacc accgccagtg cgccgctgct gccgatcagc
      241 gtgcgcatca gccgcgcgcg ccaggggcgc ggcgcggcgg cggcggccgg ctggatgccg
      301 agcttgagcg cctccaggtg cacgatgtag agcagcccca ggtacgacag cgaggccggc
      361 agaaaggcgt ggcgcaccac gtcggagtac ggaatgccga cgtattcgac catcaggaag
      421 gccgccgccc ccatcaccgg cggcatgatc tggccgttga ccgagctcat ggtttcgatg
      481 gcgccggcct tgacgccgcc gtagccggcc ttcttcatca tggggatggt gaagatgccg
      541 cccgacacca cgttggacac cgacgagccc gaaatcatgc cgttgagcgc cgacgacacc
      601 accgccacct tggccggccc gccgcgcagg tggcccagcg cggcaaagct gacctgcatc
      661 atgtagttgc cgccgccggc gcggtccagc aaggcgccga acagcacgta gatgaagatg
      721 gtgctggccg acacgcccag cgcgatgccg tacacgccct cggtggtcag ccacatgtgc
      781 gacagcaggc gcgacagcga cgcacccttg tgcgccagca cctcaggcat ccaggggccg
      841 gccacgcagt aggccagaaa cagcgccgcc agcgccgcca tcggccagcc gaccgcgcgc
      901 cgcgtggcct ccagcagcag cagcaggccg gcgctggcgg tgacgatgtc catgacgctg
      961 ggctggccgg gccgcgtggc cagctcgcgg tagaacagca tcaggtaggc accggcaaag
     1021 gcgccggcga tggccagccc ccaatcgacc agcggcacat ggcggcgcgg cgaggagcgg
     1081 aacgccggcc aggccagaaa cgccagcgcc agcgaaaacg ccagatggat ggcgcgcgcc
     1141 tcggtgtcgt tcaggatgcc ccagccgagg gcaaagggca gcggcgaggc gtaccagaac
     1201 tggaacaccg cccaggccag cgccagtccg aacatcagcc gcgccgtcaa gccactgggc
     1261 ttgcgcccgc cggtgtcggt ttcggccacc agctgctgca gcgcctcggg tgctttttcg
     1321 atgtcggtgg ccatggcgtg tctgtcggtt cactatgtta tggatagctg ccggcgcttg
     1381 atcggcgggc gctggagcca ctacatgcca tgaacggccg ggccatcaaa aagggcgaag
     1441 tcgccttcgc cctgatcgcc gtgcatgtgc gtgcggggca cggcccggcc gcgccatggc
     1501 ttacttgatc cagccctttt ccttgtagta gcgcgcggcg ccgtcgtgca gcggggcgct
     1561 caggccgttc ctgaccatgt cctcgggctt gaggtgggcc agcgccgggt gcagcttctt
     1621 gaactcgtcg aaattgtcga acaccgcctt ggtgacggcg tagatggtgt cggccggcgt
     1681 cttgctggag gccaccaccg tagccagcac gccgtaggtg ggcgtggcct gcgggttgtt
     1741 ggggtacagc ccggccggga tgctgacctt ggcgtagtac ggcttgtcgg ccaccagctt
     1801 gtccaccacc gggccggtca gcggcaccag cttggcaccg cagctggtgg tcgggtcctg
     1861 gatgttggcg ctggggtggc cgacgccata gaagaagccg tcgatcttgc cgtcgcacac
     1921 ggccgggccg tgctcgtcgg ccttcagctc ggaggccagc gagaagtccg acagcttcca
     1981 gcccatggcg gcgagcagct cctccatcga ggcgcgcgtg cccgagccgg ggttgccgac
     2041 gttgaagcgc ttgcccttga agtcctcgaa cttggtgatg cccgcttcct tgcgcgccac
     2101 cacggtgaac ggctccgggt ggacggcgaa cacggcgcgc agatcggaca ccgcgccacc
     2161 gtccttgaac tggctcaggc ccttggtggc gttgtactgg acgtcggact gggcaaagcc
     2221 caggtccagc tcgccggcct tgatggtgtt gacgttgaac accgagccgc cggtggattc
     2281 gaccgagcag cggatgccgt gcttggcgcg gtccttgttg accaggcggc agatggcgcc
     2341 accggcggcg tagtacacgc cggtgacccc gccggtgccg atggtgacga acttctgctg
     2401 cgccgacgcc ggcgccgcca gcgcggcggt ggacagagcg gcagcggcgg ccagggtctt
     2461 gagaacgggg cgaaggctca tgcgacaagg tctcctgtga atgaatgggg catgctcagc
     2521 cctgcgcgat cgccgcatcg acggccgcgg ccagccgttc ggcgatcagc gccagttcgt
     2581 catcggtggc gatgaacggt ggcgccagca ggatgtggtc accatagcgc ccatcgaccg
     2641 ttccgcccat gggataaacc agcaatccgc gcgcgaacgc ctcgctcctg acgcgcgcgt
     2701 gcaggcgccg cgcggggtcg aacggggttt tgccggcgcg ctcggcgacc agctcgatgc
     2761 cccagaagta accacgcccg cggacctccc ccacatgcgg gtggtgggca aacctttcgg
     2821 ccagcagcgc ttgcaggacc tgcgcgccgc gctgcacttt ttgcagcaat ccatcgcgct
     2881 cgatgacctg ctgcacggcc agcgcggcgg cgcaggcgac ggcgtggccg aggtaggtgt
     2941 ggccgtgctg gaaaaacccg ctgccggccg acatggcggc cacgatccgc tgttgcgcca
     3001 gcaccgcgcc gatcggctgg tagccgccgc ccagcccctt ggccacggtc atcaggtcgg
     3061 gcaccacgcc gtcctgctgg caggcgtgca gggtgccggt gcggcccatg ccgcacatca
     3121 cctcgtcgag gatcagcagc acgccgtggc ggtcgcacac ctcgcgcacc gccttgaagt
     3181 agcccggcac cggcaccagc acgccggcgg tggcgccgcc cacggtttcg gccacgaagc
     3241 ccagcacctt gccggggccg acgcggcaga tcgcctcgtc cagctcgcgc gccaggcgct
     3301 ggccgtactg ctcgggcgtt tcgcccggct gctggtcgcg gtaggggtag cagggcgaca
     3361 cgtgggtcac gtccaccagc agcggcgcaa actggcgccg gcgccattcg ttgccgccca
     3421 ccgccagcgc gcccagggtg ttgccgtggt agctctggcg tcgggcgatg aagtggctgc
     3481 gctcgggctg gccgatttcc aggtagtact ggcgcgccat cttgagcgcc gcctccaccg
     3541 cctccgagcc gccgctgacg aagtaggcgt ggctcatgcc cgccggcgcg gtgcgcacca
     3601 gctgctcggc cagctgctcg gccacctcgg tggtgaagaa gctggtgtgg gcgtaggcca
     3661 gctggtcgat ctgccggtgc atggcggcca gcacgtccgg gtgaccgtgc cccaggcagg
     3721 acacggcggc gccgcccgag gcgtcgaggt agctgcgccc ggcggcatcg tgcagccaca
     3781 cgccctggcc gcccacggcg accgggggcg tgtggtgcag gtgccggtgc atcacgtgcg
     3841 tggtgctcat ggccgtcggt ctccaggggt tcggcgcgcg ccggcgctca ctgcgccgtc
     3901 cagccgccgt cgttggccca ggcctggccg cgcacgttgg ccgccgccgc cgagcagaag
     3961 aacaccgcca tggcgcccag ctgttcgggg gtggtgaact gcagcgaggg ctccttctcg
     4021 cccagcaact cgcgcgtggc ctgctcggtg ctgatcttct cggccgcggc gcgcgcgtcg
     4081 atctgctttt gcaccagcgg cgtcagcacc cagccggggc agatggcgtt ggcggtgacg
     4141 ccggtggtgg ccagctccag cgcggtgacc ttggtcaggc cgacgatgcc gtgcttggcc
     4201 gccacgtagg ccgacttgtc cttcgacgcc accaggccgt gcaccgaggc gatgttgatg
     4261 atgcggcccc agttgcgcgc cttcatgtgc gggatggcca gccgcgtggt gtggaacgcc
     4321 gacgacaggt tgatggcgat gatggcgtcc cagcgctcgg tcgggaagcc ctcgatcggc
     4381 gacacatgct ggatgccggc gttgttgacc aggatgtcga cgccgccgaa cttctcggcc
     4441 gcgaaggcca tcatggcctc gatctcggcc ggcttgctca tgtcggcgcc gtggtaggcg
     4501 gtctgcacgc cgcaggcctc gatctgccgg cgcggtgcgt cgacgtcacc gaagccgttc
     4561 atgacgatat gggcgccctg cctggccagt tcgatggcga tgcccaggcc gatgccgctg
     4621 gtcgagccgg tgacgagggc ggtcttgcct ttcagagaca tgaggtgatc ctttcgtagc
     4681 tggaacatga acaccggacg caaaggacgc gcaacgtgcg caaacaacgc gaaagaaacc
     4741 aggcgttctc tttcgcgtcc ttcgcgtgat cttcgcgccc ttcgcgtccg gatggattga
     4801 agtggctgcc agccctttag ctgcttgcgc gagaagctat ggttattgga gcagcggaac
     4861 gccggtcttg ccttgcactt cgtcgaagct gacgtccggc gccagctcga ccaccttcag
     4921 gccgcgatcg gtgacgtcga acacgcccag gtcggtgatg atgcggtcga ccacgccgac
     4981 gccggtcagc ggcagcgtgc actggggcag gatcttcatg tcggtgctgc cgtccttctt
     5041 tttggcgacg tgttccatca gcacgatcac gcgcgccacg ccggccacca ggtccatggc
     5101 gccgcccatg cccttgacca tcttgcccgg gatcatccag ttggccaggt cgcccttgtc
     5161 ggtgacctgc atggcgccca ggatggacag gttgatcttg ccgccgcgga tcatggcaaa
     5221 gctgtcgtgg ctgccgaaga tcgagctgcc cttgatggtg gtgacggtct gcttgccggc
     5281 gttgatgagg tcggcgtcca cctcgtcggc cgtggggaac gggccgatgc ccagcatgcc
     5341 gttttcggat tgcagccaca cctccttgtc ggcgggcacg tggttggcca ccagcgtcgg
     5401 gatgccgatg ccgaggttga cgtaaaagcc gtcctgcagc tcctgcgccg cgcgcgcggc
     5461 catctggtct tgggtccagg ccatgtctcg atctccttga ttacttgtgc tcgcgcaggg
     5521 tgagcttttc gatgcgcttt tcgggcgtgg ggttgtgcac gatgcggtgc acgtagatgc
     5581 cgggcaggtg aatgtcgtcc ggcgccagct cgccgttggc cacgatgcgc tcgacctcga
     5641 cgatgcagac cttgcccgac atcgccgccg ccgggttgaa gttgcgcgcc gtcaggttga
     5701 agcgcaggtt gccgctgcgg tcggccacgt cggccttgac cagggccacg tcggccacca
     5761 gcgagcgctc catgacgtag gtctggccgt cgaactcgcg cagctccttg ccctcggcca
     5821 ccagggtgcc gacgccggtc ttggtgaaga aggccggaat gccggcgccg ccggcgcgca
     5881 gcttctcggc cagcgtgccc tggggggtga actccagctc cagctcgccg gccaggaact
     5941 ggcgctcgaa ctccttgttc tcgcccacgt aggagctgat catcttcttg atctggcgcg
     6001 tctccagcag cttgcccagg ccgaagccgt ccacaccggc gttgttgctg atcaccgtca
     6061 ggttcttcac gccgctgtcg cgcagcgcgt cgatcagcgc ctcggggatg ccgcacaggc
     6121 cgaaaccgcc cacggccagt tgctggccgt cgtgcaccac gcccttgagc gcgtcggcgg
     6181 ccgatggata aatcttgttc acggtctctc tccagttgca aatatcaaac gaacagcagg
     6241 ctcaggctcg gcgtgaacga tactacgtaa acgactacgt agtatttcgt aatggcgatc
     6301 cctgtccctg cggccggcga cccggcgctg gcgccaccgg actaccggct ggcctttcaa
     6361 ctggcgccgg tcggcctgat tctctcgcgc cagcgccaca tcgtcgattg caacgaccgc
     6421 gtgtgcgaca tcttcggcgc cgagccggcg caactgatcg gccagagctt tcgcattctg
     6481 tatcccagcg ccgacgaata cgagcgcttt ggcgcgcgca tcgcgccgat cctgaaccgc
     6541 gacggcctgt acgccgacaa ccgcgtgatg cagcgggtgg gcggcccgca ggccggcgag
     6601 gccttctggt gccacgtcac cggccgcgcc atcgaccgcc gcgcgccgca cgccgccggc
     6661 atctggagct tcgaggacct gagcgcccag cgaccgatgg gcgccgtgct gaccgcgcgc
     6721 gagcgcgaag tcgccgccca gctcacgtgc ggcctgacca gcaaggagat cggccgcgcc
     6781 ctgggcctga gccaccgcac cgtcgaagtg caccgcgcca agctgatgcg caagtaccgc
     6841 gccggcacca cggccgagct ggtgcaaaag ctcatgggcg ggctgtgagc cgaatcgccg
     6901 cgccttgaca aactatcaaa tcgatagctg cttgcgcttg tctggcaagc gccagagcct
     6961 attttttgtc ggattgacca gggccgccgt ccaccacctc ggcggcactg cgaaaggcct
     7021 ccaccgcgca cggcacgccg cagtagatgc tggcgtgcag cagcacctcc tgcagctcgg
     7081 cgaccgtggc gccgttgcgc agcgcgccgc gcacatggcc cttgagctcg tgctggcggc
     7141 ccagcgcggt cagcatggcc accgtcacca ggctgcgcgt cttcaggtcc agccccggcc
     7201 gctgccacac gtcgccccag gcgttgcggg tgatggcgtg ctgcatcggc tcggtgaacg
     7261 gcgtggcgct ggcgaacgcg cgatcgacga aatcgtcgcc catcacctgg cggcgcttct
     7321 tcaggccgtg ctggaacgcc gggtcgtcgg gcagtgtcat ggcggggtct cctggaggca
     7381 aaggccgtca gcataatgac ccgcaccccc accagaccag gagagcccca tgagccgcta
     7441 ccccctgccc agcctcgaca gcctgcccga agacatccgc gcccgcatcc tcgaagtgca
     7501 ggccaaggcc ggcttcgtgc ccaacgtatt tctggcgctg gcgcgccggc cggccgaatg
     7561 gcgcgccttc ttcgcctacc acgacgcgct gatggagccc gaaagcgccg gccgcagcag
     7621 ccggctgagc aagggcgacc gcgaaatgat cgtcaccgcc accagcgccg ccaacggctg
     7681 cctgtactgc gtggtggccc atggcgcgtt gctgcgcatc tacgagaaga aacccctggt
     7741 ggccgaccag gtggccacca accaccgcaa ggccgacatc agcgcgcgcc agcgcgccat
     7801 gctcgacttc gccatgaagg tcagccagca cccgcgcgag ctgggcgaag aggacttcgc
     7861 ccccctgcac gcccacggct tcgacgacga agacatctgg gacatcgccg ccatcgccgc
     7921 cttctttggc ctgtccaacc gcatggcctc gttcagcaac atgacgccga atgcggagtt
     7981 ttacctcatg gggcgggtgc cgagggaaaa aaaaccgtct taggcaccaa cggcagccgg
     8041 cgtgccgcta cgctcaagga agccacgcgg atcgcggaga aagggaaagg cctgacaggt
     8101 tgccccgtca ggcctttggg tacggacggg gtctttcgac ccctccttat tgcagcaacg
     8161 gctctcaccc cggataacca caatcgggac agagtatagc aagagggaat ttcatgacgc
     8221 actacgtgtt tgggctggac atcggcatcg cctcggtggg ctgggccgta ctcagcgagc
     8281 agcgcatcat cgacttgggc gtgcgctgct ttgacaaggc cgagacggcc aaggaaggcg
     8341 agcccctcaa tctcacccgt cgacaggcac gcctgctgcg ccggcgcctg catcgaaggg
     8401 cgtggcgcct cacgcagctt gcgcgcctgc tcaaacgcga gggcttgatc gcggatgcca
     8461 agctgttcgc caaggcgccc agcttccctg acaatgcctg gcagcttcgc ctgcaggggg
     8521 tggatcgcct gttgacaccg ctggagtggg cgcgtgtgat ctatcacctg tgcaaacacc
     8581 gcggctttca ctgggtcagc aaggccgaag aagccaaggc cgacagtgac aaggaaggcg
     8641 ggcgcgtgaa gcaagggctg aaacacaccg gcgcactcat ggctgaaaag ggctaccgca
     8701 gcgcggccga aatggtgctg gctgagttcc ccggcgccca gcgcaacaag ggcgggcaat
     8761 acgacaaggc tctctctcgc gtgctgctgg gcgaagaaat ggcccttctg ttccagcgcc
     8821 agcgcacgct gggtagcgcg catgcgagcg aacggttcga ggccttgatt ctgggcagcg
     8881 gagatcgacg cagtggcttg ttctggcggc agaagccagc gctctccggc gcggacttgc
     8941 tcaagatgct cggcaagtgc acgtttgaga ggaatgaatt tcgcgccccc aaagccagtt
     9001 tcagcgccga gcgccatgtc tggctcaccc gcctgaacaa cctgcgcgtg atcattgacg
     9061 gatgttcgcg agcgctgagc gacgccgagc gcgcggtcgt cttgagcctg ccctaccaga
     9121 ccgaggcgtt caagtacaag tcattgaaga acgccctcgt gaaggcagga ctatggagcg
     9181 aggaagtgcg ttttggtggc ctggcctacc ccagtcgcgc gcaggccgaa ggcgagaatg
     9241 ccaaagaccc agaagatcag gcgctggtga agatgcccgc ctggcacgaa ctgcgcaagg
     9301 ccttcaaggc cgcaggacag gaatccctat ggaaacgcat cagcacccct gccctggaag
     9361 gagaaccggc gctgctcgat cagattgcca ccgtgctcag cgtctataag gatggcgcag
     9421 aagtcgttca gcaactgcgg ggcctcgacc tgcccgaccc tgacgtttcc atcacggtgc
     9481 tggagcgaat cagttttgac aagttctcca gcctgtcgct caaggcgctg cggcgcatcg
     9541 tgccgctgat ggaggcaggc atgcgctacg acgaggccgt ggcgcagatc cctgagtacg
     9601 gccatcacag tcagcttgtg aagccgggtg ctgaaaagca tctgtatctg cctcctttct
     9661 atgaggccca gcgtgaatac cgcagcaaga atgatcgcgt gggctccatg aaattccgcg
     9721 acgatgccga cattccgcgc aaccccgtcg tactgcgtgc gctcaaccag gcgcgcaagg
     9781 tggtcaatgc cttgattcgt gaatatggca gtcccatggc cgtgcatatc gagatggcac
     9841 gcgatctctc gcgaccgctg gaggagcgca acaaagtcag acgagcgcag gacgagttcc
     9901 gcgagcgcaa tgacaaggcg cgcgaagcgt tcgaaagcga gaacggctac cggcccaagg
     9961 gtgccgtgtt cgaaaaatgg ctgctctatc gcgagcaact gggccaatgc gcctacagcc
    10021 atcagccgct ggatattggg cgcgtgctgg acgacccgaa ctatgcccag gtcgatcacg
    10081 cgctgcccta ttcacgcagc tacgacgaca gcaagaacaa caaggtgctg gtgcttaccc
    10141 aagagaacca gaacaagggc aaccgcacgc cctacgaata tctgaccgcc ctcgatggcg
    10201 gtcaggaagg cgagcgctgg cgcgccttcg tcgcctgggt gcagggcaac aagagctacc
    10261 gcatggccaa gcgtggccgc ctgctgcgca agaatttcgg cggggacgaa gcacgcggtt
    10321 ttatcgaccg caacctgaac gacacgcgct acatctgccg gttcttcaag aactacgtcg
    10381 aggaacatct gcaactggcg ccgcgtgcgg atggtgatct gaaccgccgc tgcgtcgtgg
    10441 tcagcggcca gctcacgggc tttctgcggg cacgctgggg cctggccaag gtgcgcagtg
    10501 acagcgaccg tcatcatgca ctcgacgccg cggtggtagc cgcctgctct catggcatgg
    10561 tcaaggcatt ggcagactac tcgcgtcgca aggaaatcga gttcatcaag gagggctttc
    10621 cggacccgga aaccggcgaa atcctgaatc cgacggcatt cgaccatgcc aagcggtttt
    10681 tccctgagcc gtggccgcac ttccgccatg aactgcaggc acggctgttt accgacgact
    10741 tggctgcgct gcgcgcagac atgcagcgtc tcggcaccta tcctgacgat gcacttgtgc
    10801 gcttgcgcac gctgtttgtc tcgcgcgcgc cgcagcggcg caacggtggt gcagtccaca
    10861 aggagaccat ctacgcgcag cccgacagcc tcaaagcccg gggcggcatc atcgagaagg
    10921 tgctgctgaa agacctcaag ctttctgacc tcgacaacct ggtcgaaccg caccgcaatg
    10981 aacgcttgta ctccgccatt cgtgagcgac tgcaagcgca tgctggcaag gccgacaagg
    11041 ccttcggccc ggacaacccg ctgcacaaac cagacaagag cggcaacccc accggcccag
    11101 tggtgcgcag cgtcaagctt gtgcgtgcca aacaaagcgg catctcgatc cgcggcggtc
    11161 ttgccaagaa cgacagcatg ctgcgggtgg atgtgttcac caaggcaggc aaatttcacc
    11221 tggtgccggt gtatgtgcac catcgggtca ccgggttacc ggatcgggcc atcgtggcac
    11281 acaaggatga gaacgactgg ccacatatgg acgagacata ccagtttctt cattcaattt
    11341 atccgaatga ccttataaga atcactcaga aaggcgcttc ctttagcggc tattacgccg
    11401 gatgtgaccg cggatctgga aacgtgaacc tttgggcaca cgaccgcgct atagccgtcg
    11461 gcaaagatgg gctgattcgc ggtatcggcg tcaaaacagc cctgtccctg gagaaatttc
    11521 acgttgacgt gcttggccgc atctaccccg ctcctcctga ggtccgttgt ggtctggcgt
    11581 agcgtagtca tcaaccagcc ggcccgactc aagcgcgaac atttcgcgct ggtcgtcgag
    11641 caggagcaaa gtgcccgtgt gccgttcgag gacattgccg tcatcgtgct caaccatcgc
    11701 gaaatcacgt tgacgcatcc ggtcctctcg gcctgcgccg actacggtat aggcctgtac
    11761 agcaccggcg acaaccatca gcccaatggc atcttcatgc catttctacc gcacagccgc
    11821 gccacccgca tgcagcggtt gcaactgcaa ctggacaagc cctccaccaa gcgcgcatgg
    11881 gccagcatcg tgcgcgccaa gatcgccaac caggctcggt gcatggaact gctcggccgc
    11941 ggcgatgccg aacggctgca ttcctacgtg cggcgcgtgc gctcgggcga cgccggcaac
    12001 ctggaagcgc aggccagcag ctactacttt ccgcatgtgt ttggtcgcaa ctttcaccgc
    12061 agccaaccgg gctgggtcaa tggggcactg gactatggct acgccgtcat gcgcggcgcc
    12121 tgcgcgcgcg cactggtgca gcacggcatg ctgcccagcc ttggtctgtt tcacagcagc
    12181 gagcagaacg ccttcaacct ggcggacgac ttgatcgaac cctaccgccc ggtggtcgac
    12241 ctgcatgtgg cccagcaacc caccaggtcc gacgaaaccg aactgcgacc cgcggacaag
    12301 gtggccctga tcggtctgct gaacgtcgat gtgagcatgc cgcgtggccg catgagcgtg
    12361 ctcgcgtcca tcgagcaggc agccgaggcg ctggcccgcc tgtatgacgg tggtagtgaa
    12421 caggtgctgg aattgcccgg cctgattggc ctggcgcagc acgcgttcga gatgtagcgc
    12481 agcacgccat ggcgcaggac acgaggcggt tcatgcgcat gctggtgttc tttgacttgc
    12541 ccgtggtcac caaggccgag cggcgcgctt acacgctgtt ccggcgcttt ctgctcaacg
    12601 acggctacga catgatccag ttctcagtct acggccgcat cctgaacggc agcgatgcgg
    12661 tggacaagca catgaagcgc ctgctggcca acctgccccc tgagggatca gtacgggtgc
    12721 tgacagtcac cgaaaagcag ttcgcgagca tgaaactgct ggtcggtctg cccctgtttc
    12781 aggaaaaaaa ggtcaacgca gcgcaaattg cgctgttctg aaacgcattt cggaagaaca
    12841 aaaacccgcg ctccgtcaag cggagcgcgg gattcgcaag ggccagagtg tagctatccg
    12901 gggtgagaga gggagctaca acttgcatgg caaagcctcg ttttttaacc atgagtgtag
    12961 ctatccgggg tgagagaggg agctacaacc gaacttgtcc atgatcgcgg ccaccttgag
    13021 agtgtagcta tccggggtga gagagggagc tacaactacg cacggcgtgt acacgtgcat
    13081 caaggcgagt gtagctatcc ggggtgagag agggagctac aacgcgcagc tcgcgtcgca
    13141 cgccggccag gtcgagtgta gctatccggg gtgagagagg gagctacaac gaacatcaag
    13201 ctgaaagacc tggacccgaa gagtgtagct atccggggtg agagagggag ctacaacctg
    13261 cactcacgat ggcagcagca cgctgacgag tgtagctatc cggggtgaga gagggagcta
    13321 caacgaagat ccgggagggc atcgtcaccc gagagagtgt agctatccgg ggtgagagag
    13381 ggagctacaa cgacgccgga tgcggtcacg gtggtggaaa tgagtgtagc tatccggggt
    13441 gagagaggga gctacaactc gtgttgatgg cgaaccaggc ggtattgtga gtgtagctat
    13501 ccggggtgag agagggagct acaactcata acttcggcgg ttgtttgacc aatgcgagtg
    13561 tagctatccg gggtgagaga gggagctaca accccggcca gcgttgtact gctgaccggc
    13621 gcgagtgtag ctatccgggg tgagagaggg agctacaact cgagtccccg ttgctcgaca
    13681 acgtgacgtg agtgtagcta tccggggtga gagagggagc tacaactgag cgctccatga
    13741 gcgtcgagac cctgcagagt gtagctatcc ggggtgagag agggagcggt gacctttgcc
    13801 aggcgggtaa acccgccggg caaaggtcag cgcttcgcgc aggtcaacca aagcagatcc
    13861 tttggtaccc atggaaggcg cttcaccaat cgtcgctgct cgcgcgtatc gatgctgggc
    13921 agcaaggcaa cttaacggcg tgagtcagct cgaccgtgca agccacgtgt gcctgcggcc
    13981 cgttgggcac ccccgcgctc catcccgccc acggcacccc ccgatacaat gcccgctccg
    14041 cgcgccgccg tagttcaatg gatagaacga gcgcctccta agcgctagat acaggttcga
    14101 ttcctgtcgg tggtgccacc cgcgccacgt cgctgccgcc cgccgtccac gtccgtgccc
    14161 gcctacacct tcgaagccct gcaagccgat ggcaagtccc gccagggggt gatcgaggcc
    14221 gacagcgccc gcgccgcgcg cgccctgctg cgcgaacagg cgctggtgcc gctgacggtg
    14281 gagccggtga ccaccggcgg ctcggtgctg acgcgcccgc tgggcggtgc gcgcgccttc
    14341 aacgccagcc agctggcgat ctggacgcgc cagctggccg gcctgatcgg cgccggtctg
    14401 ccactggagc gggcgctcac cgccctggtg gacgaggccg agcacgagcg ccagcgccac
    14461 ctgctggcgg cgctgcgcgc cgaggtcaac gccggcgcgc cgctggcgcg ggccatggcg
    14521 cagcacccgc gcgacttctc gccggtgtac acggccgtca tcgcggcggg cgaacagggc
    14581 ggccatctgg gcctggtgct ggagcgcctg gccgacgacc tggaagagcg ccagacgctc
    14641 aaggccaaac tgatcggcgc ggcgctgtac ccggccatcg tgaccgtggt ggcgatcgtg
    14701 atcgtggtgt ttctggtcag ctacgtggtg ccgcaggtgg cttcggtgtt cgccggcagc
    14761 aagcaggccc tgcccacgct cacggtgctg atgatggcgc tcagccagtt cgtgcgcgcc
    14821 tggggcgggt ggttgctgtt agctcttggt ttgatagctg ttggcgcacg cctggccttg
    14881 caaaacgacg gtttgcgcca gaagttcgac gccgcctggc tgcgcctgcc gctgatcggc
    14941 cggctggcgc gcggctacaa cgcggcgcgc tttggcgcca ccctggccat gctggccggt
    15001 gccggcgtgc cgattctgcg cgcgctgcag gcggccggcg agacgctgca caaccgcgcc
    15061 atgcgcgccg acgccgagga cgcgctggtc atggtgcgcg aaggcgcgcc gctggcggcg
    15121 gcgctggcgc aaaagccccg ctttcccggc ctgctggcca tgttcgcccg catgggcgag
    15181 cagaccggcc agttgcccgc gatgctgcaa cgcgccagcg cccagttgtc ggccgaagtg
    15241 cagcgccgcg ccctgcagct ggccaccgtg ctggagccgc tgctgatcgt ggtgatgggc
    15301 gtggtggtgc tgctgatcgt gctggcggtg atgctgccga tcatccagtt gaaccagtgg
    15361 gtgcggtgat gcacgtcgcg cacgagcttg ccgaccgcgc ccccacaccc gtgcgccgag
    15421 gaatcatccg agtccttctg cgttctctgc gtcatcgttg cgccctctgc gttcggatgt
    15481 cccatgccat gcggccgcaa tgaccgtcgc ttgcagtcag gttgcgcccc cgcgctcgcc
    15541 tcggcggcgc gggcggctac catgctgaca tgaccatcgc cctggacgac aagctggtcg
    15601 tcgccatctc ctcgcgcgcg ctgttcgatc tggaagagga aaaccgcctg ttcgaaagcg
    15661 gcgaccccga aggctatatg cgcgtgcagc tgcagcggct ggacaccccg gcgcgccccg
    15721 gcatcgcgca cgccatggtg cgcaagctgc tgcgcttcaa cgaagacggg gtcgagcgcg
    15781 tcgaggtggt gatcctgtcg cgcaacgacc cggtcagcgg catgcgcatc ttcaactccg
    15841 gcgcggcgca ggcgctgcgg ctggagcgcg gcgtgttcac ccaggggcgc gcgcccttcg
    15901 gctacctgcg gccgctgcgc gccgacatct ttctgtcggc caacgccgac gacgtgcgcg
    15961 aggcgctggc ggccggcttt ccggccgcgc acgtgatggt cgacgccgcc ccggccagcg
    16021 accgttaccc ggacgaggtg cgcattgcct tcgatggcga cgcggtgctg ttttccgacc
    16081 aggccgagca ggtgttccag aagaaggggc tggcggcgtt ccagcgccac gagcagcgca
    16141 aggccgacga gccgctgccc gacggcccct tcaagccctt tctggtcgcg ctgcaccggc
    16201 tgcagcaggc cggcgtgccg cacatgcgca ttcgcaccgc cctggtgacc gcgcgcggcg
    16261 cccccgcgca ccgccgcgcc atccagaccc tgatgcgctg gaacatccgg gtcgacgaag
    16321 ccatgttcct gggcggcctg cccaagggcg agttcctgcg cgagttcgaa cccgacttct
    16381 tcttcgacga ccagagcggc cacatcgccc acgccgcccg ccacgtgccg gccggtcacg
    16441 tgcccgaagg tgtgaccaat gccacgccgg cggcggaaac ttcgcgccgc cgccgcccct
    16501 ccaagcgccg atgaaaccgt ccttctggca cctgcccctc ctgctgctgg ccgtctgggc
    16561 ctcgcccgcg ccggcccaga ccgctgccgc ggcgaccacc gcgcccggcg ccgcccgcgt
    16621 cagcaagggc gaggcgcagg cactgaagat cttccgcatg ctcgacgccg acggcgacgg
    16681 ccgcatctcg cgtcaggagg cgcaggtagg cattcgcctc aagccttcgc tggccgagga
    16741 cttccggcgt gccgacctga acggcgacgg ccacctgacc gagcaggaga tccgcagcgt
    16801 cgccgaacgc cgccgcgccg agcgactgga gcgccggcgc aaggagcagg cgctggccgc
    16861 acgccccgcc accgagccgc gcagcggcag cgccgtacgc ccctgagcct ttcatcggcc
    16921 gctggcatga cggtcattcc atgcggctga gcagggcgcc gacccaaggc tgcatgaact
    16981 gccccgccag caggcccacc agaccggcgg cggcgatcag cagcatcgac agcgccagct
    17041 ggcggatcag cgcgccgctg cgcgagcgca gcacgaacga cagactgaac acgatgctgg
    17101 tgaacgaggc cagcaccgcg cccaccccgg ccgtgcccgg cggaaggccg ccctgcgccg
    17161 ccagcgaggc ggccgccgcc accgagctgg cgctggacag caggccaccg acgatgctga
    17221 cgaagtaaaa cccggcgtcg ccgaactgcc gctgcgtcag cgcgcccagc gcgtgcagca
    17281 gcagaaagat cgccccgtac ttgagcgcct gcggcagcga aaacggcatg tccaggtgaa
    17341 tttcgggcgc caccccggcg gccgaacggc gaccgctgcg caggctgacc cccaccagca
    17401 gcgcgctggc ccccatcatc agcaggaagg cggagaaggc gctgatgagc gcctcgggcg
    17461 ccaggatcag cagcagcgtg gcgttgcgcg cgatcatcgc caccatcgcc agcatgatgc
    17521 cgcggtaggc cgtgccgacg taggccacgc ccacctcgcg cacccgttcg accagctcga
    17581 tgacggtgaa gttgctgttg atcaggccac caaaaaagcc cgacagctcg gtgccgcgcg
    17641 tgccgtacat cttccacagc acgtagttga agaagccgat gccggcgatc aggatcaccg
    17701 ccacccaggc ggcgcgcggc tcgatcagcc cccagggacc gacggtgccc tgcggcagcg
    17761 ccggatagat cacgatggcc agcaccgcca gcaggatggc cgagcggatc tcgccctccg
    17821 acacgcccat ggagaagtcg gccaggcgct ctttccaggc cagcagcgca gtggcaatca
    17881 ccatcaccgc cgccggcgcg atggtgtggc cacgcccgca cataatgccg gccatgccgg
    17941 tgaccagcat ggcggccgag gtggtcagct cggtgccctg gttggcacgc aacgacgaga
    18001 cgttgaggat cagcaccagc agaccggtca gtgccagcgt ggccagcccg tagcccatgc
    18061 ccagcgcccc gcccatggtg cccagcaggc cgatgaagcc gaaggtgcgc aggccggcct
    18121 ccttgccgcg gcgctcgcgc tccagcccga tcagcaaccc cagcgccagc cccagcgcca
    18181 gccgcatgag gatctgggtg tagggccagg cactgacggg cagcagggta tcggtgtcgg
    18241 acatccgggc gatcttgccc tatggatggg gcctgcgcaa tatgacaaag tcgcgcgcgt
    18301 gggcccgccg cgacgggcct tcagccccgc ttatggattc ggcctttgga tgtcttgaat
    18361 accgttcggg ctgagcttgt cgaagccgtt ggacaggctc agggcgaatg ggtcggattt
    18421 caagaccgga agcaagtcaa cgccccaagc catggttagc atggctccct gctccctgct
    18481 ccctgctccc tgctccctgc tccctgctcc ctgcccacca tgcccatgcc ccaacgcctt
    18541 gcccagttcc agcagtttct gcgccaggtc tggcgcctgt cggcacccta cttcagcgcc
    18601 tcggacgaac gctggcgcgc ccgcgggctg ttgacggcca tcgtcgtgct caacctgggc
    18661 gcggtgtaca tgctggtgca gatcaacgaa tggaaccgcg tgttctacga cgcgctgcag
    18721 aacaaggacg ccgccgtgtt ctggacccag ctggggcgct tcacgctgct ggcgatggct
    18781 ttcgtggtga ttgccgtcta tcgcttctac ctgacgcagc tgctgcaact gcgctggcgc
    18841 gcctggatga cgcgcgacct gatgggccgc tggctgcagg gccaggcctt ctaccgcatg
    18901 gagctggcgc gctacgccgg cgccgccgtg cccaccaccg gcgccaccgg cctgccccac
    18961 cccggccagc gcgagcccga caaccccgac cagcgcctgc aggaagacgt gaacctgttc
    19021 accgcgcaga ccgtggggct gtcgatgggc ctgctcaacg ccgtggtcac gctggccagc
    19081 ttcgtcggca ttctgtgggg gctgtcgggc gcgttctcgt tcacgctggg tgaacgaacg
    19141 atcgagctgc caggcttcat ggtgtggctg gcgctgctgt actgcatcgc cggcagcgtg
    19201 ctgacgcact acatcggccg cccgctgatc gggctgaact tcgagcagca aaagcgcgag
    19261 gccgactttc ggcatcacct ggtgcgggtg cgtgaataca gcgaggccat cgccctggac
    19321 cgcggcgagc gggtggagca gcgcaacctg gacacgcgct tcacccacct gctggccaac
    19381 tacctgcgcc tgatcggcaa gcagaagcag ctggttggct tcaccaacct gttcggtcag
    19441 gcggcggtgg tgttcccgtt catcgtcgcc gcgccgcgct ttttttccgg cgccatccag
    19501 ctgggcgagc tgatgcagat cgccagcgcc ttcgaccgcg tgcagggcgc gctgtcctgg
    19561 ttcgtcgaaa actacgacag cctggccagc tggcgcgcca ccaccgagcg cctgaccagc
    19621 ttcgaagccg cgatttcaaa gcaaaatacg gccgcagagc ccacccagca agcgccagca
    19681 gctactgatt caggagttat cgaagcgcat ggcctgaacc tgcgcctgcc caccggagcc
    19741 agcctgctgg ccgcaaccga gctgcgcgtg gcgccgggtg agcacacgtt ggtgcagggg
    19801 ccgtcgggca gcggcaagtc cacgctgttt cgtgccctgg ccggcatctg gcccttcgcg
    19861 caagggcagg tggcgctgcc ggcgcgcacc atgttcatcc cgcagcggcc ctacgtcccg
    19921 gacggcgcac tgcgcgacgc gctggcctac cccgaaccgg ccagccgcta cagcgacgcg
    19981 cagctgcagg acgcgctgcg cgacgccctg ctgccggcgc tggcggcgcg gctggatgag
    20041 cgtgacaact ggacgcagaa gctcagcggt ggcgagttgc agcgcctggc cattgcccgc
    20101 gtgctgctgc ggcaaccggc ctgggtgctg gccgacgaag ccaccagcgc gctcgacgtg
    20161 gcggccgagc agcagctcta tgcgcgcctg aaggcgctca ccgaacggac cggcggcgcc
    20221 ctggtatcca tcgcccaccg gcccggcgtg gcggcctttc acgaccggta ctggcgcttc
    20281 gtgccgcaaa ccgaagggtc cgccgcacgc taccgcatcg agtcggcggc gctggcctga
    20341 gccccagggt caggcatggg tggccagatc ggccgcatcg cccgtcccat cggcgccacc
    20401 agccacaccc aatgggagca tcagaagcgg tgcgtgtagt acacctgcac gaagtccatg
    20461 ccggggttgg gctccttgat gcccaggttg gacacatgct ggtagcgcag ccccagcgtg
    20521 gagcgcgcct gcgtgcccca ttgcacgccg acgcccagca tgtcggagaa ctggaacgcc
    20581 gtgctgagcg aacgctgggg cgccacccgg gtgcgggaca gcaggcgggc accgatcgag
    20641 ccttcgagga acagcgtcgc atgtgcgccg tcgagcgggc gctccagccg cagcaccggc
    20701 gaatagccca gctccaccag gtcgcgcgcc cgcggcacat gccaggccgc cagctcgatc
    20761 tcgtggcgca gccgcaggcg ccactgggtg ccctgccaca acggcgccgc ctgggtccag
    20821 ccggcaatca ggccgagctt gcgcacaccg gcatcggccg ctgcggcggc cgacaggctc
    20881 cagtccaccc cttcggtcac ctgggcctgc acggcgccgg cgccacccca caggacggac
    20941 agcagcagcg ccgtgccgcc agcccggcgc aggtggcgcc atggcgggcc ggcgggccgg
    21001 cgcggcacgc tgcgcgggtg gcagcgggtg gtacggtggg cgtgtgccgt catggcggca
    21061 tcgtacacaa gccgcagcct gggcgaaacc gcctgtaccg cgcgcgcaac aagcgccgcc
    21121 agctatcaaa aacgcaacgt acgaagcctc aaggcgccag cgtgctgacg tactgcgccg
    21181 gcagcggtcg cgcccgcgcc ggcctggcct tgagcaccgt gcccacgctg acgaagcaca
    21241 gcgcctgttc cgcctcgccc agcccgaaca gcgtgcgcag gggtgccgac gccagcgcct
    21301 tgccgctggt cagcgccgcc ccgaagccca gcgcgtgcgc catcagcagc aggttctgaa
    21361 tcgcgcagcc ggccgagacg atgcgttcgt gcggcggaat gtcggcatcg tcgtctcgca
    21421 ggcgcaccac cgccagcatc agcaagggag cgcgaaacgc cttctcgcgc gcctgcccga
    21481 tctgctcgtc cgtggcctgg gggtcgcgct gcaccagggc cgccgcgaac gcctcggcca
    21541 ggcgcgggcg cgccgcgtcc ggcaccagga tgaagcgcca gggcagcagc gtgtgatggt
    21601 cgggcgcgct ggcggcggcg cccaggatgc gctcggtctg cgctgcatcc gggccgggca
    21661 ggcccaggcg gcgcggcagc accgtgtggc gaccgtggat cagcgcctgt gccagctcgt
    21721 cgctgccgcg cgggcgtacg gcggcggtca ggggaacgag gtcggtcatg cccggcagcc
    21781 tggcctgacg ctcgaatcgc cgggtctgca caaggtcacc ggtgggggca cagggtgtac
    21841 caggatcatc gtcatacgca cagtcaagcc cgggcattcg gtccgtcacg acgtccctgc
    21901 cacgaacccg ttccggcccc gtcgccggcc gcgaaccatg tccgcaccct acaccggcac
    21961 gcagcagcca tcagccgcag ctgcccacgt agccgcactg cgtgcagtag tcgcagccgt
    22021 ccttgcggat catggcgtgg gcgccgcatt cggggcattt cttgcccgcc atcacgcccg
    22081 gcacggcggc cgaggctgac gcctcgggct cggcctgcag caactcgtcc ggctcgaaca
    22141 gcgatgcctg cgcggcctgg gcacggcgga cgatgaggtt ttcgatggcg tagccgatgg
    22201 ccgccacttc gctgtcgtgc cagcgcggca cgtgggtgcc gtcggccttc tggtaggtgc
    22261 ccaggcgcac cgggccgcgg tcccaggtga ccttgcgcat gtccgacagc gcgcggtcca
    22321 gaaaaccgcc gcgcgccgcc aggctgagca tgcgcatggt ggcggtgatc cactgctgcg
    22381 actcgccgct ctgccccacc ggcatgaaga attcgatggc gcgttcgatg gtgcccttgc
    22441 cgtcggcgct gggcaccggc aggaaggtga cgatcaggta caggcgctgc tggccatcgc
    22501 tggtccagta ttcgatcttt tcggccaccg ccggcaggcc accggccggg cgcttttcga
    22561 tcaccgtgcg catggggtcg tagggggtga cgggcagcgg cgccggcgcc tgggccaccg
    22621 gctcctcctt cttggccggc gtggtttcga gcaccgcacc caggatgctg ttggggcggt
    22681 aggtggccag ccccttcaac ccggcctgcc aggcctggcg gtacagatcc ttgaagtcct
    22741 cgtaggggta atcctcgggg atgttgacgg tcttgctgat cgaggtgtcc acatagggct
    22801 gcaccgcctg catcatcgcc acatgctcgt gcgcggtcat ttcgagcgcg ttgacgaagt
    22861 actcgggcag cgtctcgggc tgctcgggcg ttgcgccggg atcgatcagg ctgcggtaca
    22921 ggcggaacgc gtggtcttcc accatgtagc tgctggtgct gccgtcgtgc tcgcgcttgt
    22981 tgcgcttgta gccccagctg aaggccggtt cgatgccgtt gctggcgttg tcggcaaagg
    23041 ccaggctgac ggtgccggtg ggcgcgatcg acagcaggtg gctgttgcgg atgccatgct
    23101 ggcggatggc ggcctggatg tcctcgggca ggcggctggc gaaggtgccc tgggccaggt
    23161 agccgtcggc ctggaacttg ggaaacggcc ccttttcctt ggccagctcg accgaggcgc
    23221 ggtaggccgc gtcgcgcatg cgccggccga tctcggccgc catctggcgc cccggctcgc
    23281 ggtcatagcg cagcttgagc atgctgagcg cattgcccag gccggtgaag ccgaccccga
    23341 tgcggcgctt ggaggcgctt tcggcgcgct gctgatcgag cggccagaaa gtgacgtcga
    23401 gcacgttgtc gagggcgcgc acctgcaccg ccaccacctg ctcgaaggcg tcgaagtcga
    23461 agctcggctc gccgccggcg ccgaacgggt gacgcacgaa gttggtcagg atgatggggc
    23521 cgaggtcgca gcagccgtag ggcggcagcg gctgttcgcc gcagttgtgc acgtacaggc
    23581 cgttggcgtc gaaggcgtgc acgtcggcga cggtgacgtc atagacatcg gcactgccat
    23641 ccggcgtgac ggcgctgacg gtggcggtga aacgttcgcg gttcagttgg cgctggtatt
    23701 gggccagggc gccggtcaag cgcgccgcct tgtccgtgtc ggcaaaaccg atgcgatcgg
    23761 cgaacagggc caggttgtcg ccgctgacca ccagttcgtg ctgcgccgcc gtgcggtagc
    23821 aatccaggcc accgcggcca tcgggcagcg cgcgcacgcc ggtcgggcgc cgctcggggt
    23881 aaatcgtgct ggcgatgccc aggcgcagca gcatgcgctg cgccgcctgc aaggtgccca
    23941 gatcgctttg cgccaggcgc acgctcacgc ccttgtcttg cgcgccctgc accgagccgt
    24001 cggtgtcgaa cagaccgcgc agcgcaccga tgtggaacgc cgacgaggtt ttctccatcg
    24061 acggcgtgac gctcttgcgc cccggcgtga ggccgtggct caatgccaga tgccgcaagg
    24121 cgccgctggc catgcggcgt tctgcgcgac cgcggaccgc acgctggaag ccgccgaagt
    24181 cggcgcggtg cggcagggtg cgggcggccg cctcggcggc ggcgatgatg ccgtcggcgc
    24241 cgctcagcgc atgcgccggc gcgccttgcg ccacctggcg cagctcgggc gcccagaccg
    24301 agatgacggc cttgtcctcc ttcagcacac catcgccgat cagcaggcca agcaggtagc
    24361 cctcggcctc ggtgcccgcg ccgtcccagc cttgcagcgt gcggtgctgc tgcagcatga
    24421 tttcatcgcc cgggcgcagc tggccggcgg gctcccacgc actctcgatc acgtagcgtg
    24481 tcttgcgcgt gacgcggcgc acgcggtggt ccgcggtcag gcgcaggctg tgcccttcgc
    24541 gggtttgcag gtggaccacc ggtttgtggc cggtggcgaa gaagccctcg ctgagcagcg
    24601 gatgggccgc gccgtcgacg atggcgttga agggccgacc catcaggtcg gccacctggg
    24661 cggggccatc ctcggtcatg acccaggtat cggcggtgac gcaggggttg gtggcctcga
    24721 tctgctcggt gtagttgagg ttgttgtcgc gcccgatctg gtcgagaaac aggatgcccg
    24781 gttcggcaaa atcgtaggcc gacttcatga tggtgtccca cagctcgcgc gccggcacct
    24841 tgcggtacac ccacagaccg tcggcgcgct ggtaggcccc cttggccttg accttgtcgc
    24901 ccggctcggc cttgtgcgcc agctcccagt cgctgccgtc gatcacggcc tgcatgaagg
    24961 cgtcggacac gcccacggag acgttgaagt tgttccagcg cccgggcgtg cgcttggcgg
    25021 tgatgaactc cagcacatcg gggtgatcga tgcgcagcac gcccatctgc gcgccgcggc
    25081 gcgagccggc gctttcgacc gtcgagcacg actgatcgaa cacgttcatg tagctgcacg
    25141 ggccgctggc gaacgagtgc gtgcccttga cgaaggcgcc cttggggcgg atgcggctga
    25201 aatcgtagcc cacgccgccg ccgcggcgca tggtttcggc ggcctcgcgc agagcctcgt
    25261 agatgccggg gtagccgtcg gcgtcgcggc cctggatggc gtcgcccacc ggctgcacga
    25321 agcagttgat cagcgtggcc tggatgtcgg taccggcggc gctcatgatg cgaccggcac
    25381 cgatggcgcc ggcatgcagg ttggccagaa acttctgttc ccattcggcg cgcagctcgg
    25441 gcttttcgac gctggccagg gcgcgcgcca cccggccgta gagctggtcg agcccggttt
    25501 caccgggctt gaaatacttc tcggccagca cgtcgcggct gatcggctgc gcgctcaggg
    25561 catgctgcgc gccgggcagg gaatctcttt tcatgcactc atctcctcgg atcgacaggt
    25621 cgacctgggc acgcccggtc gtggctgccg acattcttgc acctccggaa ttcgccatcc
    25681 gggacgcaac tgtgctacct gcgccacagg ccctccggat agctatgaaa tcaggagctg
    25741 cttgcgctga tcagacaagc gccagggcca gttttttcgc aacttgctga agccggaacc
    25801 gagcgccccg aagtgcgctg caccagtgcc gcccggctgg ccagccaggg ccacggcgcg
    25861 cacggaaacg ccgcgccgcc gcagcccggc ggctcagccc gtgcgcccgt agccgtcgcg
    25921 cagatcgcgc ttgagcacct tgccgatcgg gctgcgcggc agttcgggca gcaggtgcag
    25981 gtcggccagg cgctgggtct tgccggcgcg ctggttgaac cagtgcatca gggcggccgc
    26041 ttcgaccgcc tgcccgggct gcggcacgac aaacgccacc ggcgtttcgc cccaccgctc
    26101 ggagggcaca ccgaccaccg ccacgtcggc caccgccggg tgctggcgca actgggcctc
    26161 cagatcgctc gggtagatgt tgaacccgcc gctgatgatc atgtccttgc gccggtccag
    26221 cagcgtcaga aagccttcgg cgtcgaagcg ccccacatcg ccggtgcgga tgaagcgctt
    26281 gccggcggcg tcgaaccact cggcctcgcg ggttttgtcg ggctggttgt ggtagccggt
    26341 catcatgccg ggcgagtggc cgaccacttc gccggcctcg cccgaggcca cctcgcgccc
    26401 ttgctcgtcg atcagccgaa tgtcgtgacc ttgggccggc cggcccacgg tgtgcagttt
    26461 gtccggatgc aggtgggcgt ccaggatgca ggtgccgccg ccttcggtca tgccgtagaa
    26521 ctcggtcagg ccgcccggcc agcgcgccag cacgtcggcc ttgagggccg ccgaaaacgg
    26581 tgcgctggtg cagaacttga cccggaacgc ggacagatcg aatgcgccga actgcggcag
    26641 cgccatgatg cgctggtact gcaccggcac cagcatggtg tgcgtgacgc ggtgctgctg
    26701 cgccagttgc aggtaacgca gggcgtcgaa cttgggcatc agcaccaccg cgccgccata
    26761 gcccagggtg gggaagaaca ccaccagcgt ggtgttggag tacagcggcg tggacagcag
    26821 cgtgacgccg tgcggcccgt agtcgtacac cgcgccgcgg cgcacgtgca tccagcgcat
    26881 gccgtgcgac tgcacgatgc ccttgggcgt acccgtggtg ccggacgaat agatgatgtt
    26941 gaagggcgct tcgggcacca gcgccaccgg ggcgggttga gcccccaccg gcgccagcca
    27001 gtcggcaaag gcgcgcccgg gcgccacgcc atcgagcgaa acccgcggca gccggtccgg
    27061 cccgggcagg tgctgggccg gcgcggtggc gtcgagaaac agcacgcgcg cccgcgcatc
    27121 ggccaccatg gcggccagcg cctgcggcgt cgatgacggc gccagcggcg ccaccaccac
    27181 cccggcgcgc agcgcgccca ggtacagcgc cgcatacggc acggcgttga gcccgcacac
    27241 cacgatcgcc tcgcccggcg acacaccatc gcgctgcagc gccgcggcca cgcggtccat
    27301 cagcgcgtcc agctcaccat agctcagctg ctgccgatcg tcgatcaggg ccgggtgggc
    27361 cgggtcgcgc cgcgcatgct cgcggatcag gtcggcaacg gcacggaaag cgggttcggc
    27421 ggacatggcg gacggggctg cggtcggtcg gcaccagggc gtgttcagtc gttcaggccg
    27481 agcgactgca cgatggattt ctcggcctcg aaattcctgc gtgcgaactc ggcataggcc
    27541 tggtggtcgc ggtagtcggt gcgcacacca taggtcttga tgacgcgctg catgtccgga
    27601 tcgtccagcg cggccttgac cgcggcgtca agcttctgga cgatggccga cggcaggccc
    27661 ttcgggccgg ccaaccccag cggcgacggc accaccacgt cgtagccggc ttccctgaac
    27721 gtgggcacgc ctgcgaaggc cggcgggcgc tggtcggccg cgatggccag cgcccgtacc
    27781 ttgccggact ccatgaagct cagaacgccg ttggtgctga cgatggtctt cacatgaccg
    27841 cccagcaggg ccgcggtgga ttcggaatcg cccttgaacg gcacatgcac gaacgacacg
    27901 ccggcggcct tggccaacaa cgccatcgcc acctggttgc cggtgaactt gccgggcgtg
    27961 ccgtagtcca cagcgtcctt ccggccccgg gcgtgctcga ccacgtcctt gaccgacttg
    28021 aacggtgagt cggccgccac ggcaagaatg aagtcgtagg cggtgaacga ggcgatgtag
    28081 gtcagatcgc ggatcgggtc gtagggggtc ttctgaacgt agggctggcg gaacacgctg
    28141 accggcacca gcgtcaggcg gtagccgtcg ggcttggcgc gcatcaggtc gaccacgccc
    28201 agcgtgccac ccgcaccgga cttgttctcc accaccaccg gctgcttgag ttgcctggac
    28261 aggctggcag ccagcgcgcg acccagcacg tcggtggtgc cacccggcgc gtagggcacg
    28321 atcatggtga tcgactggtt gggatagtcg gcctgagcct gaaccgcggt ggcgtaccac
    28381 gcccccatgc atgccaccac cagggtgagc agacgtcgct tcatggttgc tccgctccaa
    28441 aacccgagat gttgcctttg tcgcgatccc gcggcaaccc tcggtgacga tccgaccggt
    28501 gccggtggcg tcgagcgccc acccgcacca agccggcggc accgtggccc acggccgcct
    28561 cagaagatct cgaacagtcc ggccgcgccc atgcccccgc ctatgcacat ggtcaccacg
    28621 ccccatttca cgcgctcgcc gcgtgccttg cggcgctggc cctcgagcag gatgtggccg
    28681 gccaggcgcg cgcccgtcat gccaaacggg tggcctatgg agatggcgcc gccgttgacg
    28741 ttgagccgct ccatggggat ggcaagccgc ctctggcagt acagcgcctg cgaggcaaag
    28801 gcctcgttca attcccacag gtcgatgtcc tcgaccttga ggccggtgcg ctcgagcagg
    28861 cgcggcacgg caaacaccgg gcctatgccc atctcgtcgg gctcgcagcc ggcaatggcc
    28921 aggccgcgga aggcgcccag gggctgcagg ccgcgccttt cggcctcttg ggcctccatc
    28981 agcacgcagg cggcggcgcc gtccgacagc tgcgatgcat tgcccgcggt gacgaacttg
    29041 cccgcgccct tgacgggctc gagccctgcc aggcctgcca gcgtggtcgt ggggcggttg
    29101 cagttgtcca tggtcacggt cacttccttg tgcgtgacct cgcccgtgtc cttgttcttc
    29161 tcggccatgg tggtcttgca gggcacgatt tcgttgtcga atacaccgcc ggcctgggcg
    29221 gcggccgtgc gctgctggct ttgcagcgca aaggcgtcct ggtcctcgcg cgagatgccg
    29281 tagcgcgccg ccaccacgtc ggcggtgtcg atcatctcca tgaacagctg gggcttgtgc
    29341 tcgaccagcc aggggtccag gtgggccgtg ccgccgcggc ccgcggggtt gagcagcgag
    29401 atgctctccg cgccgcccgc gaccatggcc ggcacgccgt ccatcacgat gcgcccggcc
    29461 gccacggcga tggcctgcag gcccgaggag cagaagcggt tgatcaccgt gccgcccacc
    29521 gtcaccggca ggccggcgcg cagcgcggtc tggcggccga tgttgcggcc catcgtgccc
    29581 tcggggtagc cgcagcccca gatcaggtcc tcgatcaggc cgggctctat gcccgcgcgc
    29641 tcgacggcgg cgcgcaccgc atgggcgccc agttgcgggc cgggcgtgat gttgaactcg
    29701 ccgcggtgcg acttggccag cggcgtgcgg gcggtggaaa caatgacggc ttggcgcatg
    29761 ggaggctcct tgggaatctg gtgttactat tgaattgata gctggttgcg cttgatgggc
    29821 gggcgctagc ggcatttttc ttcacaaacc aacagcgctg cgtagctacc ccctccccct
    29881 ctgggggagg gttggggtgg gggccagcgg cgctcagaac ctcggtgccg gcaagatgcc
    29941 ggcgctcccg gcccccatcc cagccttccc ccaaaggggg aaggagcaaa tcatcagccg
    30001 cgctgattca ggctggcgaa gtttttgccc tcggccacca gtcttttcag cagggccgcg
    30061 ggctgccaga actgcgcgtc ttccttggca aattcctcga tgtcggccag gaccttgggc
    30121 aggccctgca tgtcggccca cagcatgggg ccgccgcggt ggcgcgggaa gccgtagccg
    30181 aacagaaagg tcacgtcgat gtcgagcggg cgcagggcaa tgccctcctc gagcaccttg
    30241 gcgccctcgt tgaccatggc cgccatgtag cggcgcatga tctcctcgtg cgtgaaggcg
    30301 cgcggcgcga tgcctttctt ttgccgctcg gcgtccacga tggccagcac ctcggggtct
    30361 tgctggccca cgcgcgcgcc ctcggggtag aggtaccagc cacgcccggt cttctggccg
    30421 aaccagccgc gctcggcgat gcggtcggca atctccacat agcgcgccct ggggtcgcgc
    30481 gtggcggcct tgcgtttgcg cgtggcccag ccgatgtcgc cgccggccag atccgacacc
    30541 tggaacggcc ccatgggata gccgaactcg cgcaccgcgg cgtcgatctc gtaggggctg
    30601 gcgccgtcct cgagcaccgc gttggccgcg tcgcggtaga cggccaggat gcggttgccg
    30661 atgaagccgt cgcacacacc ggcgcgcacc ggcaccttct tcatgcgctt ggccagctcg
    30721 aacgccgtga ccaccacgtc gggggccacc ttggcgggca cgacgatctc cagcagccgc
    30781 atgatgttgg ccgggctgaa gaaatgcagg ccgatcacgt cctgcgggcg ggacgtcact
    30841 gcggcaatgg cgtcgatgtc gaggtaggag gtgttggtcg cgagcacgca gccgctcttg
    30901 cacacgcggt cgagctcccg gaacacggcc tgcttgaccg cgatgtcctc gaacacggcc
    30961 tcgatcacca ggtccacgtc ggcaatgtcc tcatagcgcg tcgagggctg gtagcgcgcc
    31021 atggtggcgg ccttggcctc ggccgtcatg cggcccttgg cgatcaggcc gtcgtagacc
    31081 ttctccacgt tcttctggcc gcgcgcaatc gactcggcgt cgcgctcgat catcaccacc
    31141 gacaggccgg cgtcgagcgc agacaccgtg atgcccgcgc ccatggtgcc gccgccgatg
    31201 atggccagct tcgcaaacgg gcgcggcgca gccgccttgg cctcagggat cttggcggtc
    31261 tcgcgctcgg cgaagaacgc atggaccagg ccggcgcgct gcgggctgtt caggcattcg
    31321 acgaagagct cgcgctcgcg cttcgcaccc tcggcaaacg gcgtctgcac ggccacacgc
    31381 acgcagtcga tgattcgggc gggcgagaac aggccgcgcg tcttcttggc cgtgtcctgc
    31441 gccagctggt cgagcgcggc cagggtctgg gccttgtcgc cgggcacggc cagcgcgctg
    31501 gtgcgccgcg gcgcggtacc ggccgcgagc agctcgcgca cataggccag gcccgcggcc
    31561 agcgggtcgt cgccctccac cagccggtcc accaggcctg cggccagcgc ggccttggcc
    31621 gagaggtgtt tgcccgacag catgagctcg gccgcgggca gcgcgcccat caggcgcggg
    31681 ctgcgctgcg tgccgccggc gccgggcagc aggcccaggg cgacctcagg caggcccagc
    31741 ctggccgctg gcagggccag gcggtaatgc gtggccatgg ccacctccag cccgccgccc
    31801 agcgccgggc cgtggatggc gcagacgacg agcttgtcgc aggcctccat gcggttgatg
    31861 gtttcgggca gcgagggcgg ctgcggcggc ttgccgaact cgcggatgtc ggcaccggcg
    31921 atgaaggcct tgcccgcgcc cacgatgagc atggcgcgcg cctgggcggt ccgctcgagc
    31981 tcctcgacgg cggccagcag gccggcgcgc acggcctggc ccagtgcgtt gacggggggg
    32041 ttgtcgatgg tgacgaccag cacgtcaccg tcgaggcggg tctgcaccgg gccggcgccc
    32101 tgtgaagcgt tcatgcgctt gtctccatgc gagttgtagg catccctgca ttctccccat
    32161 gggtggtttt tttgacaatc acatcagcgg ttgacaaact gtcaaacggc atttgacatt
    32221 gcaagcatgg accttcagtc gctcaccctg ctggtcgaga tcatcgacag cggcaacctc
    32281 agccaggccg cgcgcaagct gcgcatgaca cgcgccaacg tcagctacca cctgacgcaa
    32341 ctggagcaat ccgtcggcgc gcagctgctg cggcgcagca cgcgccacgt cgagccgacc
    32401 gagatcggcc tgcgcctgta tgaacatggc cgcgccattc agaacgaaat gctggccgca
    32461 cgcgagaccg tgacccgtct gggccagggc ctgatggggc gcgtgggcat cagcatgccc
    32521 agcggttacg ggcagatggt gatgtcgcac tggctgatcg agttcaagcg gcgctacccc
    32581 ggcatcgtgc tcgacgtggt gttcgagaac cgggtcgacg tgctgcgcga cgacgtggac
    32641 atcgcggtgc gcgtgctgcc cgagccgccg ccacatctgg tggcacgcga gctggacgag
    32701 gtgcgctacc tggcctgcgc ctcgtcggcc tttgcccgca cccatggcct gcccacccgc
    32761 ctggagcagc tgaccacgct gccgctgatc accgccagcg tggtcgggcg gcagctgtcg
    32821 ctgcgcggct accggcagga cgagcggcac gaggtgctgc tggcgccgac cctgatctcg
    32881 gaccactacc cgtttctgcg ccagggcatt ctggccgggc tgggtctggg cctggtgccc
    32941 gactatgtgg tgcaggacgt ggttgccagc ggtgcggtgg tgaccgcgct ggacgacttc
    33001 cggctcagca tcttcggcac ccgcatgtac ctgctgcacc tgcccggcag gcaccagacg
    33061 cgggcggtgc gcacctgcat cgactttctg ctggcgcgcg ccgccggcca gtccgcgccc
    33121 ccgacgcccg gcgagccacc ggcctgaccc gcggcgttgc cggcgcaacc gttccgccgg
    33181 tcacccaatt actatcattt caatagctgc tggcgcttgc ctgtcaagcg ccggagcctg
    33241 attttaatta aaggtgcgct gagcccgtcc ggcgcgcgtc gcatcgcagg gacgggttca
    33301 gcatcgcctc agggctgtgc ctggaacgca cgcagcgccg cgatgcgctc ttgcatcggc
    33361 gggtgggtgg aaaacagctt gccgatgccg ccggcgatgc ccatggccgc caggttcttg
    33421 ggcatttcgg cgccttcggc atgcatgccg cccaggcgcg ccagggcgtt gatcatcgag
    33481 ttgggctggc ccatcatctg cgccgcgccg cggtcggcgc ggaactcgcg ctggcgcgag
    33541 aaccaggcca cgatgatgcc ggccagcacg ccgaacagga tgtcgagcac gatggtcgtc
    33601 acgtagtagc cgatgcccgg gccgctgctt tcgctgtcgc ggcgcaggaa gccatcgacc
    33661 agcgaaccga tgacgcggct ggcgaacacc acgaaggtgt tcatcacccc ctgaatgagg
    33721 gccatggtga ccatgtcgcc gttggccacg tgggccacct cgtggccgat gacggcttcg
    33781 acctcctcgc gcgtcatgcc gcgcagcagg cccgtggaca ccgccaccag ggccgaattc
    33841 ttgaaggcgc cggtggcgaa ggcgttgggt tcgccctcgt agatgccgac ctcgggcatg
    33901 ccgatgccgg cctgatcggc gaacttgcgc acggtgtcga cgatccaggc ctcgtcggcg
    33961 ttgcgcggct gcgcgatgac ctgcacgccc atgctcatct tggccatcga cttgctcatc
    34021 agcagcgaca cgatggcgcc ggaaaagccc accaccagcg aaaacaccat caactgcccg
    34081 gtgttggtgc cggtgtagcg gcccacgccc agcaggctgg tgacgatgcc caccaccacc
    34141 atcacggcca ggttggtcag aacaaacagc aggatgcgtt tcatgaattc ccttggttga
    34201 caacaggtga cctgaaatgc ccgccccgtg cgccgcgcgc aagcggcaag cgcttcggcg
    34261 tcgccgcaag gtcggcacag gcgtgcatgt taggggcgat tggagcagat tcaaggcgcc
    34321 gggttcccac gggcgccggg ttggccaggc gctggcgctt ggcctacact gaagcacccc
    34381 cgaggagcac acgtgaacac cctggactgg ctcggccgcc tggtggccat cgacaccacc
    34441 agccgcaact ccaacctcgg cctgatcgag accgtgcgcg acgcgctcac ggcccagggc
    34501 ctgaagccct ggctggccgg caacgccgac ggcagcaagg ccaacctgtt ctgcaccctg
    34561 cccgcggcgg acggccagac gcagggcggc atcattctgt cgggccacac cgacgtggtg
    34621 ccggtggacg ggcaggactg ggccagcgac ccgttccagc tcaccgaccg cggcgacggc
    34681 cgcctgtacg cgcgcggcag ctgcgacatg aaaggtttca tcgccagcag cctggcgctg
    34741 gtgcccgagg cgctggcggc gccgcgccgc aaaccgctgc acctggcact gtcgtacgac
    34801 gaagaagtcg gctgcgtcgg cgcgccggtg atgctggccg agttgaaccg gcgcggcatc
    34861 cgcgccgacg gctgcgtggt gggcgagccg acctcgatga agccggtggt ggcacacaag
    34921 ggcggctact tcttccgctg caccgtgcat ggcaaggcgg cgcactcctc gctcacgccg
    34981 cagggctgca acgccatcga gcacgcggcg cggctgatct gccacatccg cgacctggcc
    35041 gacgcctggc gcgcgcaggg cccgttcgac gccgccttcg acgtgccgca cagcaccatc
    35101 accaccaacc tgatcgccgg cgggatcgcc atcaacaccg tgcccgacgc ctgcagcttc
    35161 agctacgact tccgccacct gcccggccac gacgccagcg gcatccaggc ccaggtgcag
    35221 cgctacgtgg acgacgagtt gctgccgcgc atgcggcgcg agtacgccgg cgcgcgggtg
    35281 gacatcgaat gcggcgccgc cgtgcccacc ctggacgcca gcgagcaggc cgccatcacc
    35341 cagctggtgc gcgccctcac gcaggaccag ggggtgcgca aggtggccta tggcaccgag
    35401 gccggccagt ttgcccaggc cggcatcccg gccatcctgt gcgggccggg atcgatcgag
    35461 caggcgcaca agcccgatga atacgtcgag cacgcgcagc tcgcgctgtg cgatcgcttt
    35521 ctgcgccagg tgctggccac gctgtgacgc gccgccacgc ctcgtgagcc ccaccacgcc
    35581 cgccgcctgc gcccgcctga tcgcccagct gcagcacgac ctgcgggcca gcggcgcggc
    35641 cgacgtgcag tgcctcgaaa cccacatttc ctggttgctg ctggcgggcg agcatgccta
    35701 caagatcaag aagccgctga cgctggactt cctggacttc ggcaccctgg cgcagcgccg
    35761 cgccgcctgc gaggaggagc tgcgcatcaa ccgccgcagc gcgcccgagc tgtacctgga
    35821 ggtgctgccg atcaccggca cgccggcggc gccgcgcctg ggtggcgatg ccgcgcaggc
    35881 gatcgaatgg gccgtgcaca tgcgccgctt tccgcccggc gccctgctgg ccgagcgcgc
    35941 cgcgcgcggc gccctggggc ccgcccagat cgacgccctg gcgcgtgcgg tggccacctt
    36001 ccaggcccag gccgcacagg caccggccga cagcccctgg ggccatgcgg ccgatctggc
    36061 gcggctggcg cacgacaact ttgcccccct ggcggcgctg ggcgcgggca ccgcgctggc
    36121 cggcacgctg cagcggctgg cgcactggag cgagaccgag ggccggcgcc tggcgccgct
    36181 gatggccgcg cgccgcgccg ccggccgggt gcgcgaaggc cacggcgacc tgcatctggg
    36241 caacctgctg tggctggagg gccgtgcgcg cctgttcgac gccatcgaat tcgatccagc
    36301 cctgcgctgg atcgacgtcg cctgcgacat cgccttcgtg ttcatggacc tgcacgccca
    36361 tggcctggcc gcgctggcct ggcgctttct gaatgcgtgg ctggagcaca gcggcgactt
    36421 cggcgccgtg gcgcggctgc cctacgacgc cgtctaccgc gccctggtgc gcgccaaggt
    36481 ggcggcactg cgcgccgggc agggccaggg tggcgtcgcg cgcaccgccg cagaagccga
    36541 agcgcgccgc tacgtgcagc tggcggcgca gctggccgcg ccgcgccggc cctggctggc
    36601 gctcaccgtg ggcgtcagcg gctcgggcaa gagcagccag acgcagaccc tgatcgagcg
    36661 gcgcgccctg gtgcgcctgc gcgccgacgt cgagcgcaag cgcctgttcg gcctggcgcc
    36721 cgaggccgcc agcagcggcg tgcccggcgg catctacgac gaagccacca gcgagcgcgt
    36781 gttcgcccac ctgctggacg aggcgcgcgc gctgctgcag gccggccagc cggtgctggt
    36841 ggacgccacc ttcatccgcc gcgcgcggcg cgcgcccttc atcgcgctgg ccgaagagct
    36901 gggcctgccc tggcgcctgc tggccttcga cgccccggag caggtgctgc gccagcgcgt
    36961 gcgtgagcgc gccgcgcagg gcggcgacgc ctccgaggcc gacgaagcgg tgctgcaccg
    37021 ccagctggcc cagcgccagc cgctgacggc gaacgaagcc agccacgcgc tgaccatcga
    37081 cgcccgccag ccggtcgact gggcggcctg ggacagcgcc ctgcccggcg cctgaaatac
    37141 tccaaattca atagctgctt gcgctgtctg gaagcgccct gaagcacgat ttcatctcga
    37201 attcaggcgc accccggctc ctacggtgtc gcgtcctcac accgggccca tgacaccggc
    37261 tgcgccggca tgacccatgc cccatgggtc agcgaaaggc agcgaggtca tctgtcgttc
    37321 gtcattgtga aaaagcgttc gcttccaccc gcagcccggg cgcggccgcg cggccggaac
    37381 acctcggcgt cgtcatagaa ggccggctgc gcgttgtccg gccaccagcc cggcacgccc
    37441 agcagcggca gcggcacgaa gggcttgcgc gccagcttgt cgggcgtcag gtcggccgcc
    37501 agccaggcgt ccagcgccgc cagctcgccc gcggcgtcga acggcagcgc cacgcgccac
    37561 acatgggcgg tgatcgactt gtagggcgaa agcagctttt ccagcgcggc gtggccgaac
    37621 accagcaggc tggcctggcc ccacagcggc cgcagcggcc cgaacagctc ggcccagcgc
    37681 cgctcgacca gcgcctgcca cagcgcgtcg ggcgcgtgca gcagggcggc gttttcgtcg
    37741 aacacggtgc aggcgtcgcg caccggcccg cggcgcgcgc ccacgccggc gcgagcgatc
    37801 tcgtcggcct ccagcgcgtt gagcctgccc ttggtgcgcg gcaggcgcag ccacaccaga
    37861 ccgttgaaca gatcgtgggc gttgtcgcgc gtgggcacct ggcgccgggc gcggatgaag
    37921 gcttcatacg cctggccctc cggcagcgcc tgctggccca caaagcgcac cggcacgtcg
    37981 ccctgcgccg ccgccagcgc gttcaggccg tcggctaccg gggcgcccgg cgccagcccc
    38041 ggcggcggcc aggccgccag cagcgcccgg tacggctgcc accacggcgc ctccagggcc
    38101 gcggcggacg gccagcgaag cgacaggtca gcggggatgc ccatggcccg attgtccgcc
    38161 cctgcggcgc cggcgggccg gcggccgtgg gctgcttctt gagtcaggtc aattcaacag
    38221 accgggcgac ctacaatggc cgtcgcgccc cacgcccggc ttccatcggc ccgttcgacc
    38281 ccgcgtgcag gggcgcccgt cgacgcccgg cccgcgccga cggatcccac agatcgggcc
    38341 atgcccaccc ctggagccgc accatgccca ccgatccgcg caaacgcgcc ttcctccgaa
    38401 tcgccgccgc ctccgccctg ggcgccaccg cactggccgg ctgcggccgc aaggacgagg
    38461 ccggcgcgcc ggccgcggcc ccggtcgccg caccaccggc cgccgccggg ccgctgaagg
    38521 ccgcctggat ctacgtcggc ccggtcggca atgccggctg gagctttgcc cacgaccagg
    38581 gtcgcctggc ggtgcaggcc gcgttcggtg accgggtcgc caccagcttc gtcgagagcg
    38641 tgcccgaggg cgccgacacc gagcgcgtgc tgcgcgacct gatcggccag ggccacaccc
    38701 tgatcttcgg caccagcttc ggcttcatgg aggccatggt caagctggcg caggagcatc
    38761 cgcaagtccg gttcgagcac gccaccggct acaagacggc accgaacctg aacgtgtacg
    38821 actcgcgctt ctatcaggac acctacctgg ccggcatcat cgccggccac atgacgcaaa
    38881 gcggcacgct gggctacgtc ggctcggtgc cgatccccga ggtgctgcgc aacatcaacg
    38941 ccttcaccct gggcgcgcaa agcgtcaagc cccagatcag aacgcgcgtg gtgtggatca
    39001 acagctggta cgacccgccc aaggaaaccg aggcggcgca gtcgctgatc aacgccggcg
    39061 ccgacgtgct gctgcagaac accgattcgc cggccgtgat ccaggccgcc gacaaggccg
    39121 gcaagctggc tttcggctgg gattcggaca tgagcgcgca tggcggcgcg gcgcacctgg
    39181 cctcgtgcat cgccgactgg agccactact acaaaaaggc ggtgcaggcg gcgctggacg
    39241 gcagctggaa gggcgggcag gtcacgcgct ggggcgtgcc cgaaggccag aacgacctga
    39301 tcaagctgtc ggaaaaaatc ccggccgagg tgcgcgccca ggtcgaggcc atcaaggcca
    39361 aaatgaagga cggcagcttc gatgtcttca ccggcccggt ggtcgacgtg gcgggcaagg
    39421 aacgcctggc cgccggccag cgcgccgacg acgcctggat gggcgccatc gacttctttg
    39481 tcaagggggt cgaaggccag atccctgctt caaaataaat agctgctggc gcaagctgga
    39541 tctgcgccag acccctgtcg gatctggagg cgaagatgaa cctggaacgc agcctgtggc
    39601 accccgtggc gctcggccac gtggtcggca gccagccgcg cgccgtcacc ctgctgggcg
    39661 aggacctggt gctgtggcgc gacggcaatg gccacgccca cgcctggatg gaccagtgcc
    39721 cacaccgcgg cgcgcgcctg tcgctcggcc gcgtgcatgg cggccagctg gaatgcccct
    39781 accacggctg gcgtttcgac ggcggcggcc ggtgcaccca tgtgccgtcc gtgcccgact
    39841 tccagcccgg cccgcgccac ggcgcgcgca ccttcgacac ccgcgaggcc tacggcatgc
    39901 tgtgggtgcg tctggctgcg cccgagggcg agccgcagcc gatccatcag ctgcccgtgt
    39961 tcgccgccga agccgagccg cgcctgcgca agaccaccag cggcccctac ctggtggccg
    40021 ccagcgcgcc gcgcatcatc gagaacttcc tcgacatggc gcacttcggc tacgtgcacg
    40081 aaggctggct gggcagccgc gagcggcccg aaatcccacc ctacgaggtg caggccacgc
    40141 cgcacggcgt gcgcgccacc ggctgcctgg cctggcagcc gcgctcgtcg atccacgccg
    40201 caagcggcgc ccaggtcgaa tacacctatg aagtcaccgc gccctacatg gcggtgctga
    40261 ccaaggtgcc cgaagccggc agcaccgcca tcgaaggcat gaacgagtcg atcgccatgt
    40321 tcgtctgccc cgtgaccgag gaaagcagcg tggcctggac gcgcatggcg atgaacgact
    40381 tcgactcgcc cgacgcgcgg ctgatcgact tccagaacac catcttcggc caggaccagc
    40441 cggtgctcga atcgcagcgc cccaagcgcc tgccgctggc gcccgccgcc gaagcgcatt
    40501 gcgccgccga ccgcatgtcg gcggcctacc gccgctacct gcgcgaaagc ggcatcacct
    40561 tcggcgtcat cccatgagcg cgccgggccg ccccaagcgc gaatcccgca gcgcgtcagc
    40621 gcggagggat accgaatgag cgcgccgggc cgccccaagc gcgaatcccg cagcgcgtca
    40681 gcgcggaggg cgcacctgtg agctgggcgc cgccacccat cgccgcagcg cgcctgccgg
    40741 cgctgctggc ggcagccccc aaggccgagc tgcacatcca catcgaaggc tcgctggagc
    40801 cggaaatgat cttcgcgctg gcggcgcgcc acggcatcgc cctgccctac gccgacgtcc
    40861 aagcgctgcg ccgcgcctac gccttcggca acctgcagca gtttctggac ctgtactacc
    40921 agggtgccga cgtgctgcgc gatgaggagg atttttatca gttggggcgc gcctaccttg
    40981 cacgagcagc tacagaaaac atagtacacg ccgaaatctt cttcgacccg cagacgcaca
    41041 ccgcccgcgg cgtgccgatg ggcgccgtga tgcgcggcct gcgccgcgcc agcgacgatg
    41101 gccggcgcga gctgggcatc ggcagcgcgc tgatcctgtg ctttctgcgc cacctgtccg
    41161 agcgcgaggc cttcgagacg ctcgaagccg ccctgccctg gcgcgacgac ttcatcggcg
    41221 tcgggctcga ttccagcgag ctgggccacc cgcccgaaaa attcgcccgc gtgtttgcgc
    41281 gctgccgcga gctgggcctg cacgtggtcg cccacgccgg cgaagaaggc ccgcccgaat
    41341 acatctggca ggcgctggac atcctgaagg ccgagcgcat cgaccacggc gtgcaggcgg
    41401 tgcacgacgc ggcgctgatg cgacggctgg cccagcggcg catcccgctc accgtctgcc
    41461 cgctgtccaa ccagaagctg caggtgtttc ccgacctggc gcaccacagc ctgccgcagc
    41521 tgctggccgc tggcctgtgc gccaccgtca acagcgacga cccggcctac ttcggcggtt
    41581 acgtcaacgc caacttccag gcggtgtttg ccgccaccgg gctggacagc gccgccgcct
    41641 accagctggc ggccaacagc ttcgaagcca gctttgccga cgcggcgctc aagcagcggt
    41701 ggctggacaa gctgcaagcc gccttcgagc gcgcgcacga cgccggctcg tagaatccgg
    41761 ccccgcagcg ctgccgcccc gacggcgccg cgtcttcatc catcggggcc atgtagagga
    41821 acaccatgta caaaaacctc gtggccgcgc tcgcggccgc ttgctgcttc ttccccctgc
    41881 aacccgcaca agcccagccg cccacgcgcg ccgcctgggt ctacgtcacc cccgtgctcg
    41941 acaccggctg gacgcgccag cacgagctgg ggcggctggc ggtgcagcag gcgctgggcg
    42001 atcaggtcaa gaccagctat gtcgagaacg tggccgaggg cgccgacgcc gagcgcgtga
    42061 tgcgcgatct ggcacgccag ggcaacgaca tcatcttcac caccagcttc ggctacatgg
    42121 agccggcgct gaagctggcg caggagttcc ccagggtgaa gttcgaggtc atcaccggcg
    42181 tcaggcgcgc gcccaacgtg gccaccgcca acgcgcgcta ctacgaaggg cgctacctgt
    42241 cgggcgtggc cgcggggcgc atgacgaagt cgaacgtggt gggctacgtg gcgggctttc
    42301 cgatcccgga gatcctgtcc agcatcaacg ccttcacgct cggtctgcgc agcgtcaacc
    42361 cccaggccac ggtcaagctg gtttggctga acgagtggtt caacccgccg cgcgagcgcg
    42421 aggccgcgat gacgctgatc gaccagggcg ccgacgtgct ggccttcaac agcgcctcca
    42481 ccgccgttat ggccgccgcc gaggagcgcg gccagctggc catcgcctac cactccgaca
    42541 tgcgcgccgt ggcgccgacg gcgcagctgg ccgccgtgac ccaccactgg gaggcctacg
    42601 acctggcccg cgtgcgcgcg ctgcgcgccg gccagtggca gagcggcgac agctggggcg
    42661 gcgtggccgc cggcatggtg cgcatcggcg ccttcggccc caaggtgccc gccgccgtgc
    42721 gccaggaagt gctggcgcgg cagaaggaca tcgccgccgg acggctgcag ccgtttgccg
    42781 gcccggtggt cgacaacacc ggcaggccgc gggtgcccaa ggggcgcagc gccagcgacg
    42841 ccgaaatcct gcagatggac tacctggtcg aaggtgtgca gggcagcttg ccgaagtaga
    42901 catccgacca gcgaacgcag aggttgcgga ggtttcgcaa agcgcacgaa cagaagagca
    42961 acttcatgga cttgttctgc gcatggcgcg cgatcccggt gttcggcaga ccgcggattc
    43021 agccgccgac aaatcaggca aaatacggcc ccagcgcttg tccattgtgc gcaagcagct
    43081 atcgatttaa gagtgacgca gcatgagcat caagagcgac aggtggatcc gccgcatggc
    43141 cgagcagcac ggcatgatcg agccctacga gcccggccag gtgcgcgagg tggacggccg
    43201 cagaatcgtc agctacggca ccagcagtta cggttacgac atccgctgcg cacccgagtt
    43261 caagatcttc accaacatcc acagcaccgt ggtcgacccg aagaacttcg acgaaaagag
    43321 cttcgtcgat tttcatggcg actgctgcat catcccgccc aacagcttcg ccctggcgcg
    43381 cacggtggag tacttccgca tcccgcgcaa cgtgctgacc atctgcctgg gcaagagcac
    43441 ctacgcgcgc tgcggcatca tcgtcaacgt cacgc
//
LOCUS       DAOOOG010000006        39185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_786_length_39185_cov_25.352159, whole genome shotgun sequence.
ACCESSION   DAOOOG010000006 DAOOOG010000000
VERSION     DAOOOG010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 39185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39185
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_786_length_39185_cov_25.352159"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="PK242_01130"
     CDS             <1..1481
                     /locus_tag="PK242_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOZ92661.1"
                     /translation="QQHCASCHGEQRLGGMGPALLPESLARLRKPEALKVIRDGRAAT
                     QMLPFASALKADEIAALAEWIYTPVTPPPRWSDADIAASREETAGWRDWPARPRWQAD
                     PMNLFVVVEGGDHHVSLLDGDRFEVIHRFASRFALHGGPKFTPDGRYVYFGSRDGWIT
                     KYDLWNLKVVAEVRAGINMRNIAVSGDGKWVMAANYLPHTVVLFDAELKHRRTYAARS
                     LDGSQSSRVSAVYDAEPRRSFVVALKDIPELWEISYDPKAEPIYDGFVHDYKMKEGLP
                     KDGFLGVRRTVLDEPLDDFFFSQNYAYALGASRPHADGSPSGQVVNLDVRRKIATLPI
                     AGMPHLGSGITFDRGDTRVLASPNLQDGVISVIDMKSWQLVKNIATPGPGFFMRSHEK
                     TPYAWTDSMMSPKAKHILTIIDKRTLAPVARIEGTPGKTLAHIEFTKDGRYALASLWE
                     DDGALIVYDAASFKEVKRLPMRKPVGKYNVFNKITRSEGTSH"
     gene            complement(1497..2195)
                     /locus_tag="PK242_01135"
     CDS             complement(1497..2195)
                     /locus_tag="PK242_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ92662.1"
                     /translation="MDLKKFNLPRYLATLPLFSNMRDEELARLASGCQLRRMERGKLV
                     FRTGEPCQEFHVTVLGQVKLFAIAPNGTEKVIELCGPGHSFAEAVMFMDMPYMISAQT
                     LTDSLILTVGKAAVLGEIAANPDFSLRMLAGLARRLHGLVKDVEAYTLHSGVQRVIGY
                     LLGDRVSESEVASESITVSLPVSKAAIASRLSLTPEYFSRVLNELETAGLIQVDKRDI
                     RIPDTARLANHQPL"
     gene            complement(2252..4219)
                     /locus_tag="PK242_01140"
     CDS             complement(2252..4219)
                     /locus_tag="PK242_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOZ92663.1"
                     /translation="MEEWVGERWHRFIWRAADRSFAHAAVRLEEVQRAVQLLFRAGGG
                     DPLVRVAPASAEKVGGPRSWLQKLAGQGERASIGTLDEQTLALPPVIAVFADAALNRD
                     LYLWLAAQAACHVATPAGWLADNLAATRRALACFPGLRTRYARLLQAHLAQRPAPERL
                     QGTPRELERAIQAALRASLDGVDAGRDTTPPAAREPCAPQDQGASLDGRARQPASVAP
                     VWLWLQAMQTAVGPSATAPEADAADGAPPRPQQQPTPQDPKRRRAQRTEDTSSKHGLV
                     MFFRAESILSWSEFVRVNRTADDEDNQDYSQVADDMQELALAPRKPGETLAARIKFDL
                     DLPSAAADDLPVGEGEPLPEWDYQRGLLLPEHCRAQRLRHRAASPDAIDSEAAGAVPA
                     LEKRLKTTSRQVRRRFEALRAAPRWQHGLTQGEELDLDAWVRHQVDRRDVAHRHVEAP
                     AVYARRVRSERSLSSLMLADLSLSTDAYARPGARVIDLVRDSLFVFGNALDAVGDPFE
                     MLGFSSVRRAVRIHELKRFGERWNDAARERVAAIKPGFYTRMGAAIRYGTQRLAARPE
                     RQRLLLILSDGKPNDLDHYEGRHGLEDTRHAVHAARQAGLVPFCVTIDQQANAYLPRL
                     FGTQGFALVHRPEQLARALTQVYVRLTAAVR"
     gene            complement(4259..4918)
                     /locus_tag="PK242_01145"
     CDS             complement(4259..4918)
                     /locus_tag="PK242_01145"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HOZ92664.1"
                     /translation="MAARLGRPLVTVSCHDDLTAADLVGRFLIGDGNTVWNDGPLARA
                     VRTGAICYLDEVVEARKDTTVVLHPLADDRRVLPIERTGELLQATPEFMLVVSYNPGY
                     QNLLKNLKPSTRQRFVALVFDYPRPEVEQRIIETEAGADAALSARLIKLATALRRLID
                     QDLEETVSTRLLVVAARLIVSGLPPLTACRAAIVDALTDDLDTASALDQVVQAVLSHD
                     A"
     gene            complement(4914..5009)
                     /locus_tag="PK242_01150"
                     /pseudo
     CDS             complement(4914..5009)
                     /locus_tag="PK242_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5198..6655)
                     /locus_tag="PK242_01155"
     CDS             complement(5198..6655)
                     /locus_tag="PK242_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOZ92665.1"
                     /translation="MSNNTILKYKSQAVAKPYFIAAIALFVGQILFGLVMGIQYIYGD
                     FLFPLIPFNVARMVHTNLLIVWLLFGFMGAAYYMVPEEAETELYSPGLARLMFWIFLV
                     AGALTVIGYLLVPYARLAELTGNNLLATMGREFLEQPLPTKVGIVIVALAMLFNISMT
                     YLKGRKTTITTVLLLGLWGLALLFLFAFVNPHNLVRDKMYWWWVVHLWVEGVWELILA
                     ALLAYVLIKTTGVDREVVDKWVYVIIAFALFTGILGTAHHYYFIGLPGYWHLVGNIFS
                     SLEPIPFFLMTMFAFRMVQRRRRDHPNQAAVLWAVGCAVLGFLGAGVWGFLHTLSFVN
                     YYTHGTQITASHGHLAFYGAYVLVVIALISYAMPALRGRIANSYQAQALEMWSFWVMS
                     IGMGVMVLALTGAGIVQVWLQRMPESGAMPFMAAQDQVVFFYWMRLWSGVVFLIGLVM
                     YVWSFFVGGAPAEETLIERRGGLMPSPAQRRLRHD"
     gene            complement(6675..7127)
                     /locus_tag="PK242_01160"
     CDS             complement(6675..7127)
                     /locus_tag="PK242_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOZ92666.1"
                     /translation="MSSTASGGFTKEMARNIFYGGSLFFILIFLGLVFHTEQRLPERT
                     NQAAMTDAVVRGKAVWEQNNCIGCHTLLGEGAYFAPELANVYQRRGGDASGAVFIKAW
                     MKAQPTGTPGRRQMPQFHLSEQQLDDLVEFLKWTGGINAEQWPPNIEG"
     gene            complement(7876..9420)
                     /gene="paaZ"
                     /locus_tag="PK242_01165"
                     /pseudo
     CDS             complement(7876..9420)
                     /gene="paaZ"
                     /locus_tag="PK242_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
     gene            complement(9568..10308)
                     /locus_tag="PK242_01170"
     CDS             complement(9568..10308)
                     /locus_tag="PK242_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ92667.1"
                     /translation="MSAPLLSLPQFSVFYGPVQAIHGIELHVGEGEIVTVIGPNGAGK
                     TTLLNAIMGLLPSQGGLNLGGHAVARPSVEIMVRHGVALVPEKRELFGEMSVEDNLLL
                     GGFALWRRGQRDQAARMKEIFGIFPRLEERRAQMASTLSGGERQMLAIGRALMSRPRL
                     LMLDEPSLGLAPLIVREVLHTVSALRRTGVSVLLVEQNARAALNVADRGYVLEMGEVA
                     VQGDAGELMHDQRVIDTYLGVGGARAAA"
     gene            complement(10305..12113)
                     /locus_tag="PK242_01175"
     CDS             complement(10305..12113)
                     /locus_tag="PK242_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HOZ92668.1"
                     /translation="MKPMHLVLAFIVLLGAAWGFLPPFTVTLLSYIGLYALVAVGLVM
                     LTGVGGMTSFGQAAFVGLGAYATAWISLSPEAAQWLGALPKGAYPWVGLLLGLAVTAA
                     VAWVLGAVTLRLSGHYLPLGTIAWGLSLYYVFGNLEMLGGFTGLSGIAPLEVFGVSLA
                     SPRRLGLVIWAVLLLAIWGLHNLLDSREGRAIRALKSGRLMAESMGVDTARQRIKVFV
                     LAALLAALSGWLYVLLQRFVNPTPFALNIGIEYLFMAVVGGSGHLWGAVLGATLITLL
                     KEQLQDWLPRLLGSSGNFEIVVFGLLMLLVLQRAPNGLWPMLAGALQRFTGAAAPRRL
                     AAGAATLAQRQLPPAGELILQADKVTKRFAGLLANNAVDLDVRAGEVHALIGPNGAGK
                     STFFNMISGVDDPTEGQVRLMGQVMQGQPSRAFAALGLGRTFQHVRLLGERSVLENVA
                     LGAHRRGRQGWLASMLRLDRAEEAAVLAEAMRQIERCGLAEVAHTPAAALPLGQQRIV
                     EIARALAGQPAILLLDEPAAGLRHLEKQGLATLLTQLRSEGLAILVVEHDMEFVMNLA
                     DRITVLEFGTVIAHGTPAEVQANPRVIAAYLGGDVL"
     gene            complement(12110..13138)
                     /locus_tag="PK242_01180"
     CDS             complement(12110..13138)
                     /locus_tag="PK242_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ92669.1"
                     /translation="MDLQIAVMLVQDGIINGAIYALMALALVLVFSVTRVIFIPQGEF
                     VAYAALAMAALQAGQSPPLLWMLLGLAALVLIVELVRQTRGAEVDWRSTLFWAVLLPL
                     LAALMLLVLKPQAMLLRSLAVLLLVTPMGPLLYRLAYRPLADATVLILLIVSVALHLA
                     LVGLGLFFFGAEGSRTEAFSDARWDLLGLTVSGQSLVILGVTAALVVGMFLFFERTIV
                     GKALRATAINRVGARLMGIPTALSGDLSFALAALIGAVSGLLIAPVTTIYYDTGFLIG
                     LKGFVAAIVGGLASYPLALAGALLVGQLESFASFWASAFKEVLVFTLIIPVLWWRSLH
                     THHVEDEE"
     gene            complement(13226..14380)
                     /locus_tag="PK242_01185"
     CDS             complement(13226..14380)
                     /locus_tag="PK242_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ92670.1"
                     /translation="MLANKKALLAAAITFGCTAAWADINVGVTLSATGPAASLGIPEK
                     NTIALLPQTIGGQKINYIVLDDASDTTQTVTNTRKLITERNVDVIIGSSTTPTSLAMI
                     DVVAEAQTPMISLGASAAIIEPQDGKKRWAFKTPQNDIMMALAIAEHMAANGVKTVGF
                     IGFSDAYGEGWYRETVKALDLKKIKLVANERFSRTDTAVTGQVLKLIGAKPDAVLVAG
                     SGTPSALPQKTLKERGYTGKYYQTHGVANNDFLRVGGKDVEGTLLPAGPVLVADQLPA
                     ANPVRKSALEYINAYEGANGKGSVSTFGGHAWDAGQILKAAIPVALKGGQPGTPQFRN
                     ALRDALEGVKEVPGAHGIFNMSASDHLGLDQRARVMVKIENGRWKYQEVK"
     gene            complement(14659..15642)
                     /locus_tag="PK242_01190"
     CDS             complement(14659..15642)
                     /locus_tag="PK242_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HOZ92671.1"
                     /translation="MAFFAIALALLLEQVRPLTSDHPAAVGLHRWVRLVGRNVDAGAA
                     QHGWLAWVLAVAVPALAVSAVHLLLSWLGGWPLALVWSVAMLYLTLGFRQFSHHFTGI
                     RDALEAGDEERARGLLAHWQHVDASTVPRSEIVRHVIEVSVLAAHRHVFGVLAWFCIG
                     ALLGLGPAGAVFFRSAEYVARYWRRKAQAADQPSSPALCLAAEQAWQLINWLPARTTA
                     LGFAIVGSFEDAIEVWRSHASRFADRNDGVILAATAGALGVRLGGASLRPLTPEGAAA
                     VPVSAAGVSGEGLPGEPPRTAHFTQVVGLVWRTVALWLLLLVLLTLAHVLG"
     gene            complement(15689..16426)
                     /locus_tag="PK242_01195"
     CDS             complement(15689..16426)
                     /locus_tag="PK242_01195"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOZ92672.1"
                     /translation="MSTPTPLSKLPNFDPRQVPVTGIDAHLPPVPAEALTLPALRRRF
                     QAPPPWQPELRQEPRFTDRVPAAAAVLVPLVDRPQGLSVLLTERSGNLSTHSGQVAFP
                     GGKVDPEDADAVAAALREAEEEVDLSRRFVDVLGQLPVYVTGTQFIVTPVVAVVQPGF
                     VLRPNPREVAHAFEVPLAWLMNPAHHRRHRLTWEGQAREWFSMPYVEPLSVVAGSGES
                     LPGAAVERFIWGATAGMLRNFYRFLSA"
     gene            complement(16528..16911)
                     /gene="rplS"
                     /locus_tag="PK242_01200"
     CDS             complement(16528..16911)
                     /gene="rplS"
                     /locus_tag="PK242_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOZ92673.1"
                     /translation="MNLIQQLEKEEIERLGKSIPEFAPGDTVIVSVNVVEGNRKRVQA
                     YEGVVIAKRNRGLNSGFTVRKISNGEGVERTFQTYSPLIAKIEVKRRGDVRRAKLYYL
                     RGLSGKKARIKEKLPGRAASPTAAD"
     gene            complement(17052..17816)
                     /gene="trmD"
                     /locus_tag="PK242_01205"
     CDS             complement(17052..17816)
                     /gene="trmD"
                     /locus_tag="PK242_01205"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOZ92674.1"
                     /translation="MRFDVITLFPELFAPFLEAGVARRAYASGQIVLKLWNPRAYAEG
                     NYRRVDDRPFGGGPGMVMLAEPLARCLAAVQAERAGGAAPVVLFSPIGQTLTHAGVER
                     WAAGAGAVLICGRYEGIDQRFIDRHVTHQISLGDFVLSGGEIAALALLDAVARLQPGV
                     LGDEGSHHQDSFNLAVDGLLDCPHYTRPEVWDGVPVPSELLSGHHARIEGWRREQRLR
                     LTARHRPDLIVAARAAGRLSAHDEVLLAQLGRGGPG"
     gene            complement(17837..18823)
                     /locus_tag="PK242_01210"
     CDS             complement(17837..18823)
                     /locus_tag="PK242_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOZ92675.1"
                     /translation="MSELHKFIFDGLPVRGQLVRLTDAWQEVLRRRAANTQTGAWPPV
                     VAEMLGEMAAATVLMQGNIKFDGALIMQIFGDGPLKLAVAEVQPDLRLRATASVVQSV
                     TPGMALPELVNRHGRGRCAITLDPRRRREGQQAYQGVVPLVDEGGAPLQTLASVLEHY
                     MRQSEQLDTTLVLAADEQVAAGLLIQRLPVQGQDNLSQSSVAREDAGSAADDDYQRIR
                     LLAHSLQRAELLSLDADTLLHRLFWQEPLLRFAPLAGHQGPRFACSCSRERVAAMLQG
                     LGQPEAESILAERGEIEVGCDFCGQQQRFDAVDVARLFTPAAQQSPQPGSVQ"
     gene            complement(18841..19365)
                     /locus_tag="PK242_01215"
     CDS             complement(18841..19365)
                     /locus_tag="PK242_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOZ92676.1"
                     /translation="MAVYELDGVAPQVDPAAWIADSAEVIGNVSLGAGASVWFGCVLR
                     GDTERMMIGEGSNIQDLSVLHADVGQPLTVGRHVTVGHKVMLHGCTVGDESLIGIGAV
                     VLNGARIGKNCLVGAGALVTEGKEFPDGSMIVGAPARVVRQLTPEQIEGLRQSARHYV
                     DNARRFQAGLKRLG"
     gene            19413..20228
                     /locus_tag="PK242_01220"
     CDS             19413..20228
                     /locus_tag="PK242_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOZ92677.1"
                     /translation="MDLRPLALAALCEADVERKLALVAALQAPAATLYIAPETALEAP
                     PGLPGRPLRPPLIDPARVPQRSVHTREGRAALVHAICHIEFNAINLALDAIWRFAGMP
                     EAYYRDWLRVAIEESHHFSLLHAHLQTHHQLRYGDFEAHDGLWAMCEKTRDDLTARMA
                     LVPRTLEARGLDATPLIQRKLRAVATPDALAACDILDLILREEIGHVAIGNHWYRWLC
                     ARQGLEPLAHYRRLTRQHRAPRLRGPFNTEARRQAGFSDDELRALSADPPTPS"
     gene            complement(20262..21467)
                     /locus_tag="PK242_01225"
     CDS             complement(20262..21467)
                     /locus_tag="PK242_01225"
                     /inference="COORDINATES: protein motif:HMM:NF020322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ92678.1"
                     /translation="MESPVGLLSQPALQAALLAAAGALAYWLKDLPRLLLQWGRRFFV
                     STLTVDSRDEFLFPALVEHMDQHPGLAGVNQFTARSVRRGTEHQSLAEDLRAGQPPRA
                     FLSPGEGVHILRVDGRWLWLRRELQVGQSVFERLSLSTLGRSPRYLQDLLQAAIDARA
                     ARETDRLSVYLPNPFHGGDWMRARLGWRRPLASVVLKAGQAEALLADLRRFLASRERY
                     AELGIPWRRGYLLYGPPGTGKTSLVTALASELHLNVCTLSLASPVVTDEKIHTLLAAV
                     PQRALLLIEDVDAFFREREAAHAQVRLSFSGFLNALDGVATQEGTLLFMTTNHLERLD
                     PALIRAGRIDEQVELGHADEDQLRRLYLKFHPDPAAAAVFARERAGQALSPAAVQGEL
                     MKRFGTDPV"
     gene            21645..27632
                     /locus_tag="PK242_01230"
     CDS             21645..27632
                     /locus_tag="PK242_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOZ92679.1"
                     /translation="MHRSPALDRALRPTLLVLCGLAASVGAHALGIRSLSPQGEVARV
                     NQVVASFDQDAVRMGDAGAPAPLRVDCDQAAAARGQGRWRSAREWVFQFEADLPPGVR
                     CRVTPEPGFQSPTGAPWTGARSYSFHTGGPFVQQLVPAPTSTIEEDQAFVLRLNGEAS
                     ADSLNAHLWCAVDGLGERVPVRLVEGAPRAALLKHLGWDKAAQQTPGRYVALQCNRRL
                     SAAARVQLVFGAGVATPSGVLNRVERRFAFTVREPFSASLSCERENAQAACMPIRPLT
                     LKFSAPVTRAQAAQARLRADKLEYEPVFDEPQDGDALIEQLRFPAPLPERRSFTLHLP
                     ADLKDASGRALTNASSFPLRVSTGPLPPLAKFAAAPFGVIERFAEGPDGPALLPLTLR
                     RVEPALQAQALHIHSLQPQSDADIIAWFKRVQRYDEAYVPRALARRDLGERLPPPVNE
                     QTRDQVESRTLSLLQGRSGLNTLTLPAAPKEGERPFEVVGVPLPPGFHVLELASQQLG
                     EALLDARYGARRPMFVRSSTLVTNLAVHFKLGRAGSLAWVTALDSGKPVADALVRVSD
                     CHGKPAAEARTDAQGIARFDRLSPEPPSCSGEDEFYADFQQAWFVSARARQAGVDELA
                     FTWSSWQRGIEPWRFNVPTSSQPQPDLRAHTVFDRALLRTGETVSMKHLLRSETPGGF
                     GLPPQMPATLVITHLGSGQTFTQPLQWRRTATGGRSAESSFAIPQAAKLGVYQVTLRH
                     GGAAGADGGDDAGDIETGSFRVEEFRLPVLQGRVAPQSGEPLVAATQVPVQVQVSYVS
                     GGPAARLPVRVSALTRGKTLSFADYEAFSFEPPRPPAAGPDRSDDAEPPDAARQDQQV
                     VADKLAVTLDAQGSGQTRIEAIAAAPQARELVLEASYADPNGEIQTLRGSTTLWPAGV
                     IAGIRADSWTSVQQTLKMQALALDLDGQPRAGVPLDVKAVARSTTSSRKRMVGGFYTY
                     DNRTETRELGTLCTGRSDTRGLLTCDIRLTHPGEVELVVSARDPQGRASQAARSVWVT
                     RQGELWFGGEDHDRIDVLPEKREYAVGDTARFQVRMPFRRATALVAVEREGVMQTQVV
                     ELRGDDPTLTLKVGPDWSPNVYVSVLALRGRLVEVPWYSFFTWGYRRPRDWWQAFWHE
                     RGDYAPPTALVDLSKPAFRLGMAEIRVGTDAHRIAVDVQADQTSYPVRGKARVRIQAR
                     LPGGQPAAHAEVAVAAVDEALLELLPNRSWDVLPAMLRRRAWGVQTATAQMEIVGRRH
                     YGRKAVPAGGDGGTAPTRELLDTLLLWQPRVVLDDQGRADIEVPLNDALSTFRVVAVA
                     DSGTALFGSGHTRLRVTQDLQIIGGLPPLVRGGDRYRALLTLRNTTTRPMQVQVSPRA
                     TLVELKPQSVDIPAGEAREVGWDVTAPPELATLRSGEILWEVEARDRASGARDALKLS
                     QRVLPAVPVTVQQATLTQLEGPLTLQLAAPAAALPGRGGVGLALQPTLADGLPGVRDW
                     LARYPYACLEQQTSVALGLRDATRWQKTAAQLPVYLDADGLAHYFPPRAGDEHRGSDT
                     LTAWLLAATHEASRLDAAFALPAALRAQMVAGLTQFVEGRIERRHWSPRGDLDVRKLA
                     ALEALSRHGAVRPAMVSSLTIAPQQWPTSAVIDWINLLRRVPGIADQRTRLQEAQQVL
                     RARLSFQGTRLAFSTEQDDHWWWLMAGGDVNAARLLLSALDDPAWQADLGRLASGLIA
                     RQQSGAWTTTTANLWGSLALEQLARQREREAVAGTTQAALGDARDKVDWSKVVRLKAD
                     EPGSAAHARSLFGAPASPGQWTGNRLLLPWPEPPGAPAPLTVTHQGSGKPWLTLQALA
                     AVPLAAPVADGYQITRSVHAVEQANPALPAGQYSRGDVLRVTLDVQARADMTWVAITD
                     PVPAGATVLGSGLGRDSDLATQGERRSGQGWLAYEERSFEAFRAYYEFLPKGKLSLSY
                     TVRLNNPGRFQLPPSRVEALYAPEMHGLTPNAPLIVH"
     gene            complement(27790..29490)
                     /locus_tag="PK242_01235"
     CDS             complement(27790..29490)
                     /locus_tag="PK242_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOZ92680.1"
                     /translation="MRPHHAFWPKRLPYSLTPPATSLWQNLAINALRYPDKLATVFFG
                     TVLSWSQLKLSAERLAARLQALGVAKGDRVLLMLQNTPQLIVAHFAILRADAVVVPIN
                     PMNKAEELKHYITDPDARVAITSADLAGELAAASDALPAAQRLAHLIVTQYSDAFDAG
                     VQGDDAPPEHWRDWLLTARALPQLAGGQVHTWRDALAFGGPLAETTVGPQDLSVLPYT
                     SGTTGLPKGCMHTHGSIMHNAVASSLWGTGHSESVVLVVVPMFHITGMVSVMHSAVYS
                     AATMVLMPRWDRELAGRLISRWQVTGWTNIPTMIIDLLASPNFASFDLSSLRTIGGGG
                     AAMPAAVAQRLEEQYGLKYAEGYGLTETAAPSHSNPPDRPKQQCLGIPFMSTDARVVD
                     PETLKELPQGETGEIVVCGPEVFAGYWKRPEATAAAFFELDGKRFFRTGDLGRVDEEG
                     YFFITDRLKRMINASGFKVWPAEVEALMFKHPAIQEACVIGFKDAYRGESVKAVVVLR
                     ASHKGQVSEQDIVEWCHANMAAYKAPRAVQFVDALPKSGTGKVMWRTLQEAEASVSVH
                     "
     gene            complement(29867..30106)
                     /locus_tag="PK242_01240"
     CDS             complement(29867..30106)
                     /locus_tag="PK242_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92681.1"
                     /translation="MTMHVWLVAATLWVSLGVPAQAQPRVEYLTEDPPKGSLPHRKVV
                     YVDDRSCPKGEVKEVTGGSERQSIPRKVRCVKRPG"
     gene            complement(30213..30668)
                     /locus_tag="PK242_01245"
     CDS             complement(30213..30668)
                     /locus_tag="PK242_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92682.1"
                     /translation="MTRKTAGTLVFGILSVLGWLLTAYLARGAGASARASMFAWAAGF
                     VALILTLHTLLYALLADRKARDADWLRRHGRAVQAEVVKIGRRGKGWRVQALAVAPTG
                     TAAPTVFKSDILRADPRPRLAVGDHIQVHLHPTDRRRYWMAIGTESKYL"
     gene            complement(30760..31098)
                     /locus_tag="PK242_01250"
     CDS             complement(30760..31098)
                     /locus_tag="PK242_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOZ92683.1"
                     /translation="MKQITAIIKPFKLEEVREALAEVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVMIEVVVTDADVDRCVEAIVKSARTGKIGDGKIFVTPVERVVRI
                     RTGEEDDAAV"
     gene            complement(31116..32774)
                     /locus_tag="PK242_01255"
     CDS             complement(31116..32774)
                     /locus_tag="PK242_01255"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOZ92684.1"
                     /translation="MSLKICVAQLNLVVGDLPGNARQIIDAAHTAHAQGARLLLTPEL
                     AISGYIAEDLFLRPAFIAACDDALNRVARETAGLGGLTIVVGHPSAVAPVDDVGPRCA
                     NAASVLCEGRRVAHYAKRLLPNYEVFDERRYFTPGQGSCVFTVDGVRVGLLICEDAWF
                     DEPAAATRQAGAELLAVINASPYHQGKGAEREAAMARRARASGLPLVYANLVGGQDEV
                     VFDGRSLAIGADGQLAGRAASFKENLFFVQASRAAAAIELKADMAPEPTPEAELWEAL
                     VLGLRDYVDKNGFAGVALGLSGGLDSALVLALAVDALGAARVRTLMMPSPYTAGMSVE
                     DAHEMARRLGVRHDELSIRPAFETLRATLAPLFAGRGEDVTEENIQARVRGVLLMALS
                     NKLGHLILTTGNKSEYAVGYCTLYGDMCGGFAPIKDVAKTTAYRLARWRNAHDPHGTG
                     ANPMPERIITRAPSAELREGQTDQDSLPPYEVLDAIVARYVEDNASIADLLAEGFAAA
                     DVERVTRLIKASEYKRQQAAVGTRVTRRSFGKDWRYPMTNRYRV"
     gene            32777..34120
                     /locus_tag="PK242_01260"
     CDS             32777..34120
                     /locus_tag="PK242_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ92685.1"
                     /translation="MQAGGRPGEYRNAAQRPADAAPALQVLDTPAALDAARWNALLAR
                     QPSPTPFMRHEYLLALHASGSAVPHTGWAPRFMLLWAGSELIAACPLYLKSHSFGEYV
                     FDWAWANAHAEHGLPYYPKAVVAVPFTPVPGTRLLARDQAARAALIAAVRDWCADQRL
                     SSLHLLFGDEADLAACEGAGLMRRSQVQFHWKNGAPPRSAAAPAALPPVGALAALGRP
                     GGGSDAPAERPFADFDDFLAALTQDKRKKIRQERRRVAQAGVRFRWAAGNDITAEDWA
                     FFYRCYRRTYLEHGNAPYLTPAFFAHMARHMADVWVLFTAEREGHPIATSLIAVGARP
                     ESARGSNDAYAGAVAYGRYWGALERVDCLHFELCYYAPLDWCIAHGVARFEGGAQGEH
                     KMARALLPVATHSAHWLAHPGFADAVARFLARESLAVDGYLGQLGERSPFRRPDG"
     gene            complement(34140..34457)
                     /locus_tag="PK242_01265"
     CDS             complement(34140..34457)
                     /locus_tag="PK242_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92686.1"
                     /translation="MKLSIARLSIGAAVLGAAVGAQAACYTVLGPKGQILSESPNPPV
                     DMSLPLHLSVPERFGPGAHMVFGLLDAPCGQEVELHDGGALTPAALRPDSRRVRTPRR
                     DRE"
     gene            complement(34523..36025)
                     /locus_tag="PK242_01270"
     CDS             complement(34523..36025)
                     /locus_tag="PK242_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HOZ92687.1"
                     /translation="MLKKIVLGLLAVLLALAAGVAGNTWRQSSRQLQVAPLAPLAIDE
                     PAVADRLAQAVRLQTVSSASDAQLNADQFRALHALLQTQFPRVHATLKREIVNELSLL
                     YTWEGADPKANAIVLLAHQDVVPVAPGTEGDWQQPPFAGLVKDGFIWGRGSWDDKGNL
                     LAQLEAVERLLAEGYRPPRTIHLAYGADEEVGGHRGAARIAELLKSRGVQADFVLDEG
                     LLVLDGVMPGLKQPAALIGVAEKGYLSVRITVTAPPGHSSMPPRPGSSAIALLSAGLD
                     RLEHAPMPAGIRGVAGEMFGTLAPEMGGLARVALSNLWLFGPLVQKQLEGGASTNAML
                     RTTTALTMLQAGNKDNVLPGRAEATVNFRMLPGETREQVLRHVRQQLARVVPEGQFEV
                     TALPGGKEASQVAPTDSAPYQMLARTVREVFPDALVAPGLMVAGTDSVHYEAISRHVF
                     KFSPIRAVSDDLPRFHGTDERLGVTHYANAIRFYHRLIPQLGVPGSSVGR"
     gene            complement(36113..37513)
                     /locus_tag="PK242_01275"
     CDS             complement(36113..37513)
                     /locus_tag="PK242_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HOZ92688.1"
                     /translation="MTEALTIAVVGHTNAGKTSLLRTLTRRVDFGQVSDRPGTTRHAE
                     AVALRLDGREAVRFIDTPGLEDSVALLDFLARQSGDSRPERVRAFLAGPEARASFEQE
                     AKVLRALLEQAGCAMLVIDTREPVLPKHRAEIEILASCAKPVMPVLNFVRDGASRLAD
                     WHRALEESNLHARAEFDVVAPFTGAEQRLYADLATLLPARRAQLQDIVEALAQAERER
                     RLAACRVIASTLIDVAAQRRSLSAEAFADPEQQQAWVRDFQAQTGRHARRAVDELLTL
                     YAFGADDAELTELPLPGGRWEDDLFNPELLKQAGRRLGLGAMIGAGMGAVADVALAGL
                     SLGAATTLGATLGGAISGGWQPLWRKLQNRLDGVQELTVEDPVLWLLAERLLALARAL
                     AQRGHAAQQRLRLGAEAAGALGDDGQALIDALSAARGHPAWERGVAGALDDAERARLL
                     EALAGALRPALERQPA"
     gene            complement(37506..38951)
                     /locus_tag="PK242_01280"
     CDS             complement(37506..38951)
                     /locus_tag="PK242_01280"
                     /inference="COORDINATES: protein motif:HMM:NF022518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HOZ92689.1"
                     /translation="MRVTTSTSPSTPVAAAVLHETVRRIEDAGPLDDAQALAQAHRMP
                     GGRGAQVLARSQLLAERLGLPVEWARWRRLGAGVLLGLALLLALADLGLARAVLGQGR
                     SINAVAAFVSLLGLHALTLVLWLLGLLLPAGRRGGAPLGRLALWLSARLPLERGPHAL
                     TLLRALGAVLGRHGLLVWLTGAISHAVWALGFVIMGAVLVFGFAFHAYALTWESTILS
                     ASFFQRFVQITGALPALLGFAVPDAAAVTQAGHAAVGATAIAQSDWAWWLIGCVVAYG
                     LLPRVLLALLCGWRWRAGQARLAQPDMADPYVRRIVARLDALEPPPQVIDAEQRSAHP
                     TYAVRAGPPGAPGSLAVLGFELPPEQPWPLPGLPTGLSAGSEPPQRIAGSTADRQAAL
                     ARLQASRPESLLLVVHGPSSPDRGTARFVREAQALCHRMALLALGAGGSSGAGRWRAW
                     LDSEGFEAVALVEQAQAAIEWIASGDAAHHD"
BASE COUNT         6063 a        14197 c        13429 g         5496 t
ORIGIN      
        1 accagcagca ctgcgccagc tgccacggcg agcagcgcct gggcggcatg ggccccgcgt
       61 tgctgcccga aagcctggcg cgactgcgca agccggaagc gctcaaggtg atccgcgacg
      121 gccgcgcggc aacccagatg ctgccgtttg ccagcgccct gaaggccgac gaaatcgccg
      181 ccctggccga gtggatctac accccggtca cgccgccgcc gcgctggagc gacgccgaca
      241 tcgccgccag ccgcgaggaa accgcaggct ggcgcgactg gccggcccgc ccgcggtggc
      301 aggccgaccc gatgaacctg ttcgtggtgg tcgaaggcgg cgaccaccac gtctcgctgc
      361 tcgacggcga ccgcttcgag gtcatccacc gctttgccag ccgctttgcc ctgcacggcg
      421 ggcccaagtt cacaccggac gggcgctacg tctacttcgg ctcgcgcgac ggctggatca
      481 ccaagtacga cctgtggaac ctgaaggtgg tggccgaggt gcgcgccggc atcaacatgc
      541 gcaacatcgc cgtctcgggc gacggcaagt gggtgatggc ggccaactac ctgccgcaca
      601 ccgtggtgct gttcgacgcc gaactgaagc accggcgcac ctatgcggcg cgctcgctcg
      661 acggcagcca gagctcgcgc gtgtcggccg tgtacgacgc cgagccgcgg cgcagcttcg
      721 tggtggcgct caaggacatc cccgaactgt gggaaatcag ctacgacccc aaggccgagc
      781 cgatctacga cggcttcgtg cacgactaca agatgaagga aggcctgccc aaggacggct
      841 ttctgggcgt gcgccgcacg gtgctggacg aaccgctgga cgacttcttc ttcagccaga
      901 actacgccta cgcgctgggc gcctcgcgcc cgcacgccga cggctcgccc agcggccagg
      961 tggtcaacct ggacgtgcgg cgcaagatcg ccactctgcc gatcgccggc atgccgcacc
     1021 tgggctcggg catcaccttc gaccgcggcg acacgcgcgt gttggccagc cccaacctgc
     1081 aggacggcgt gatcagcgtg atcgacatga agagctggca gctggtcaag aacattgcca
     1141 cccccggtcc gggctttttc atgcgcagcc acgaaaagac cccctacgcc tggaccgatt
     1201 cgatgatgag ccccaaggcc aagcacatcc tgaccatcat cgacaagcgc acgctggcgc
     1261 cggtggcccg catcgagggc acgccgggca agacgctggc gcacatcgaa ttcaccaagg
     1321 acggcaggta cgccctggcc agcctgtggg aggacgatgg cgccctgatc gtctacgacg
     1381 ccgccagctt caaggaagtc aagcgcctgc ccatgcgcaa accggtgggc aagtacaacg
     1441 tgttcaacaa gatcacgcgc tccgaaggca cctcgcactg agggcgtcga cggccgtcac
     1501 agcggctggt ggttggccag ccgggcggtg tccgggatgc ggatgtcgcg cttgtccacc
     1561 tggatcaggc cggcggtttc gagctcgttg agcacgcgcg agaaatattc cggcgtcagc
     1621 gacaggcgcg acgcgatggc cgccttgctc accggcagcg acaccgtgat cgattcggac
     1681 gccacctcgc tctcgctcac ccggtcgccc agcaggtagc cgatgacccg ctgcacgccg
     1741 ctgtgcaggg tgtaggcctc cacgtccttg accagcccgt gcaggcggcg cgccagcccg
     1801 gccagcatgc gcagcgaaaa gtcggggttg gcggcgatct cgcccagcac cgccgccttg
     1861 ccgacggtca ggatgagcga atcggtcagg gtctgcgccg agatcatgta gggcatgtcc
     1921 atgaacatga cggcttcggc aaagctgtgg ccgggcccgc acagctcgat caccttctcg
     1981 gtgccattcg gcgcgatggc gaacagcttg acctgaccca gcaccgtgac atggaactcc
     2041 tggcacggct cgccggtgcg aaacaccagc ttgccgcgct ccatccggcg caactggcag
     2101 ccgctggcca ggcgcgccag ttcctcgtca cgcatgttcg agaacaaagg cagcgtcgcc
     2161 aggtagcgcg gcagattgaa tttcttgaga tccacggcag gaacgggggc cgacgccagc
     2221 tgcgccgcgc cctctcacaa agcagacgag tctagcgcac ggccgcggtc agccgcacgt
     2281 agacctgggt cagcgcccgc gccagttgct ccggccgatg caccagcgcg aacccctggg
     2341 tgccgaacag gcgcggcagg taggcgttgg cctgctggtc gatggtgacg cagaacggca
     2401 ccaggccggc ctggcgcgcc gcatggacgg cgtggcgggt gtcttccagg ccgtgccggc
     2461 cttcgtagtg atccaggtcg ttgggcttgc cgtcggacag gatcagcagc aggcgctggc
     2521 gctccggccg tgcggccagg cgctgcgtgc cgtagcgaat ggccgcgccc atgcgggtgt
     2581 aaaagcccgg cttgatggcg gccacccgct ctcgcgcagc gtcgttccag cgctcgccga
     2641 agcgcttgag ctcatgaatg cgcacggcgc gccgcacact ggaaaagccc agcatctcga
     2701 acggatcgcc cacggcgtcc agcgcgttgc cgaacacgaa caggctgtcg cgcaccaggt
     2761 cgatcacgcg cgcgcccggc ctggcgtagg cgtcggtgga cagcgacagg tcggccagca
     2821 tcaggctgga caggctgcgc tccgagcgca cccggcgggc gtacacggcg ggcgcctcga
     2881 catgccggtg cgccacgtcg cgccgatcga cctggtggcg cacccaggcg tccaggtcca
     2941 gctcctcgcc ctgcgtcagt ccatgttgcc agcgcggcgc agcgcgcagc gcctcgaagc
     3001 gccgccgcac ctgccttgaa gtggttttca ggcgtttttc caaggctggg actgcgccgg
     3061 cagcttccga atcgatagca tccggcgagg ctgcgcggtg ccgcaaacgc tgcgcccggc
     3121 aatgctcggg cagcagcagg ccacgctggt agtcccactc gggcagcggc tcgccctcgc
     3181 ccaccggcag atcgtcggcg gcggcgctgg gcaggtcgag gtcgaacttg atgcgcgccg
     3241 ccagcgtctc gcccggcttg cgtggcgcca gtgccagttc ctgcatgtcg tccgccacct
     3301 ggctgtagtc ctggttgtcc tcgtcgtccg cggtgcggtt gacgcgcacg aactcgctcc
     3361 agctcaggat cgactcggcg cgaaagaaca tcaccaggcc atgcttgctg ctggtgtcct
     3421 cggtgcgttg cgcgcgacgg cgcttggggt cctgcggggt cggctgctgt tgcgggcggg
     3481 gcggcgcgcc gtcggcagcg tccgcctcgg gcgcggtcgc ggatgggccg accgctgtct
     3541 gcatggcttg cagccacagc cagaccggcg ccacgctggc cggctggcgg gcacggccgt
     3601 cgagcgacgc tccctgatcc tgcggggcgc acggctcgcg cgccgccggc ggggttgtgt
     3661 cacgacctgc atcgacgccg tccagggacg cgcgcagcgc cgcctggatg gcccgttcaa
     3721 gctcgcgcgg cgtgccctgc aggcgctcgg gggccgggcg ttgcgccaga tgcgcctgca
     3781 gcagccgcgc atagcgtgtg cgcaagccgg gaaagcaggc cagcgcgcgc cgcgtggccg
     3841 ccaggttgtc ggccagccaa ccggccggcg tggccacatg gcaggctgcc tgcgccgcca
     3901 gccagaggta caggtcgcga ttgagcgcgg cgtcggcaaa caccgcgatc accggcggca
     3961 gggccagcgt ctgctcgtcc agggtgccga tgctggcgcg ctcgccctgg ccggcgagct
     4021 tctgcagcca gctgcgcgga ccgcccacct tctcggcgct ggccggagcc acccgcacca
     4081 gcggatcgcc gccgccggcg cgaaacaaca gttgcaccgc gcgctgcacc tcctccagtc
     4141 gcacggccgc gtgcgcaaaa ctgcggtctg cggcgcgcca gatgaagcgg tgccagcgtt
     4201 cacccaccca ctcttccatc agggccctcc ccggcgccgg cgcgctggct tcgttgcctc
     4261 aggcgtcatg cgacagcacg gcctgcacca cttggtccag cgccgaagcg gtgtccagat
     4321 cgtcggtcag cgcgtcgacg atggcggcgc ggcaggcggt cagcggcggc aggcccgaga
     4381 cgatcagccg cgcggccacc accagcaggc gggtgctgac ggtctcctcg aggtcctgat
     4441 cgatcagacg ccgcagcgcc gtggccagct tgatcaggcg cgcactcagc gcggcgtcgg
     4501 caccggcttc ggtttcgatg atgcgctgct cgacctcggg ccgcggatag tcgaacacca
     4561 gcgccacgaa gcgctggcgg gtgctgggct tgaggttctt cagcaggttc tggtagccgg
     4621 ggttgtagga caccaccagc atgaactcgg gcgtcgcctg cagcagctcg ccggtgcgct
     4681 cgatcggcag cacgcggcgg tcgtcggcca gcgggtgcag caccaccgtg gtgtccttgc
     4741 gcgcctcgac cacctcgtcg aggtagcaga tggcgccggt gcgcacggca cgcgccagcg
     4801 ggccatcgtt ccacaccgtg ttgccgtcgc cgatcagaaa gcggcccacc aggtcggccg
     4861 ccgtcaggtc gtcgtggcaa ctgaccgtga cgagcggccg acccaggcgc gccgccatgt
     4921 gctcgacaaa gcgggtcttg ccgcagccgg tcggcccctt cagcagcacc ggcaggcgct
     4981 gccggaaggc gtgttcgaac agccggcact cgccggcctg ctcggcatag aagggggtgg
     5041 tggccgggga ggtgtcggtg cggtccatgg ggctgggtgg atgacgatgc agaaaaaaag
     5101 catcgccgcc gtgcgctgcc gatgacggga aatcctgcgg agttcggatc gtcatcgcca
     5161 ccgcgccggc ggcgaggtct cagggcgcgt caggcgctca gtcgtgcctg agccggcgct
     5221 gggcggggct gggcatcagg ccgccgcggc gctcgatcag ggtttcctcg gccggcgcgc
     5281 caccgacgaa gaagctccac acgtacatca ccaggccgat caggaacacc acgccggacc
     5341 acaggcgcat ccagtagaag aacaccacct ggtcctgcgc cgccatgaag ggcatggcac
     5401 cgctttcggg catgcgctgc agccagacct gcacgatgcc ggcgcccgtc agggccagca
     5461 ccatcacgcc catgccgatg ctcatgaccc agaacgacca catttccagc gcctgcgcct
     5521 ggtagctgtt ggcgatgcgt ccacgcagcg ccggcatggc gtagctgatc agggcaatca
     5581 ccaccagcac gtaggcgccg tagaacgcca ggtgaccgtg cgatgcggtg atctgcgtgc
     5641 cgtgggtgta gtagttgacg aagctcagcg tgtgcaggaa gccccacacg ccggcgccca
     5701 gaaaacccag caccgcgcag cccacggccc acagcacggc cgcctggttg gggtggtcgc
     5761 gccggcgccg ctgcaccatg cggaaggcga acatggtcat caggaagaac gggatcggct
     5821 ccagcgaaga gaagatgttg cccaccagat gccagtagcc cggcagaccg atgaagtagt
     5881 agtggtgcgc cgtgcccagg atgccggtga acagggcgaa ggcgatgatc acgtacaccc
     5941 atttgtccac cacctcgcgg tccacgccgg tggtcttgat cagcacgtag gccagcaggg
     6001 cggccaggat cagctcccac acgccctcca cccacaggtg caccacccac caccagtaca
     6061 tcttgtcgcg caccaggttg tgcgggttga cgaaggcgaa caggaacagc agcgccaggc
     6121 cccacaggcc gagcagcagc acggtggtga tggtggtctt gcggcccttc aggtaggtca
     6181 tgctgatgtt gaacagcatg gccagcgcca cgatgacgat gcccaccttg gtcggcagcg
     6241 gctgctccag gaactcgcgg cccatggtgg ccagcaggtt gttgccggtc agctcggcca
     6301 ggcgcgcgta gggcaccagc aggtagccga tcaccgtcag cgcaccggcg accaggaaga
     6361 tccagaacat gagccgcgcc agcccggggc tgtacagctc ggtttcggcc tcctcgggca
     6421 ccatgtagta ggccgcgccc atgaagccga acagcagcca cacgatcagc aggttggtgt
     6481 ggaccatgcg ggccacgttg aacggaatca gcggaaacag gaagtcgccg tagatgtact
     6541 ggatgcccat gaccagaccg aacaggatct ggcccacgaa cagcgcaatc gccgcaatga
     6601 agtacggctt ggccaccgcc tgcgacttgt acttgaggat ggtgttgttg ctcatctggg
     6661 ttgctcctcg gcgatcagcc ttcgatgttg ggcggccact gctcggcatt gatgccgcca
     6721 gtccacttga ggaactcgac caggtcgtcg agttgctgct cgctcaggtg aaactgcggc
     6781 atctggcgcc ggcccggggt gccagtgggc tgcgccttca tccaggcctt gatgaagacg
     6841 gcgcccgagg cgtcgccacc acggcgctgg tagacgttgg ccagctcggg cgcgaagtag
     6901 gcgccctcgc ccagcagcgt gtggcaaccg atgcagttgt tctgctccca caccgccttg
     6961 ccgcgcacca cggcgtcggt catggccgcc tggttggtgc gctcgggcag gcgctgctcg
     7021 gtatggaaca ccagtccgag aaagatcagg atgaaaaaca gcgacccgcc atagaagatg
     7081 ttgcgtgcca tctccttggt gaagccgcct gaggctgtgc tgctcatgtg cgtttcctcg
     7141 ctcatttagg tggtaacccg catagggtag aacccaggtg atacccagac cttaacttgc
     7201 gtcaagaacg agcagccgaa acccagaatt tcttgaccgt catcaaccgc aggacgcttc
     7261 tatatcgata gctgctggcg ctttctggtc aatgtcggag acctgaaatc gatctcggac
     7321 aggcatcgtg ggattggatc cccgtcgtcg gccaggcgca gcacaacgcc agcgctcccg
     7381 cggggccagg cagcgcaggc tgccaagggt taaccccaag aacggccgta ggttgctggt
     7441 catttccggg ggggccggat ccatgcaggg tttggcggct tcaacctaga aacggcagtt
     7501 ggacgccccc gcccgtgccg tgatcggcgt gccaggcaag acgcaacgcc gccgcgggct
     7561 ggtgcccgca aggaggagca acgccgcatg gcgcgccgag cgcggtgcgg gcgggtgggt
     7621 agcgggctca cccaggaggt caaggccatc gcagcggcga aaccccgcat cgatgcggcc
     7681 cgcccgaact tgacaagcgg tcaactgccg ttttaaggtt caatcgcctt cacctgtggg
     7741 gagatcgtgc tgcacacccg cccgcaccgc gctcggtacc ggggcgcggg cgggcggcca
     7801 aggtctggcg tgtgggcgcg ccgcgcaccc gcccgggccg tcctgacgct gtgccggacg
     7861 ccctcaggcc ggccgatcgc ccgtgaccgc cgccagcatg gccggcgagc cctgcaccgc
     7921 agcgcgctgc agcaggtgcc tgacggcgcg cagaccgccc agctcttcgc caccaccggc
     7981 gcgaccgggg ccgccgtgct tgaggtaggg caaggccgag ccatggccgg tggcctcggg
     8041 tgcggcggtg cgatcgagga tctgcagccg gccgtgcagc tgcgccatcg ccggtatcac
     8101 ctgcgccgcc acctgggggc tgcgcgtgac cagggtggcg gccaggctgc cctgaccctg
     8161 cgcggccagc gccagcgcct gttcgatgcc gtcgtaaggc atcagcgtgc tgaccgggcc
     8221 gaacgcctcg acgtcgtgcg cggcatgctg ctgcggctgg cgcgccagca gcagcgtggg
     8281 ctggaagaac gccccaccct gcgtgccttc acccaggggc gcaaagtcgg cgtcgccgcc
     8341 gcacaccacc tcggcgccct ggcgcagctg ggccacgcgc tcggccacat cgcgctgctg
     8401 cgcgcggctg gccagggcgc ccatgcgcac gccctccagg cgcgggtcgc ccacggtggt
     8461 cttggccagg cgctccttca accgtgcggc caccgcgtcc aggtgctgcg acggcaccag
     8521 ggcgcgccga atagcggtgc acttctggcc tgctttctgc gtcatctcgc gcacgacttc
     8581 cttcacgaac aggtcgaact cctcgtcgtc cggggtcacg tcgggcgcca ggatggcgca
     8641 gttgagcgaa tcggcctcgg cgttgaaggg aatgctgcgc tgcaccaggt tgggcgtgac
     8701 ccgcagccgc gccgcggtat cggccgagcc ggtgaaggtg accacatcct gcacctgcag
     8761 gcgatccagc agatcgcccg tgccgccgat caccagctgc agcgcaccgg cgggcaggat
     8821 gccggactcg tggatcagac gcaccagctt ttcggtcaga aagctggtgg cggtggcggg
     8881 cttgccgatg cagggcaagc cggccagaaa ggtcggcgcg aacttctcca gcagccccca
     8941 gaccgggaag ttgaaggcgt tgatgtgcac cgccacgccg ccgcgcggca ccagcacgtg
     9001 ggtgccggca aaggcgccgg tcttgcccag cggcatgctc gggccttcat gccacaggtt
     9061 gccgcaggcg ggcagctcgc gcgccatgct ggcgtaggcg aacagggtgc cggtgccgcc
     9121 ttcgatgtcg acccagccgt cggcgcgcgt ggcgccggtg tgcaacgaca gcgcatacag
     9181 ctcctccttg cgctcgccca ggtacttggc cagcgccttg agcatggcgg cgcgctgcgc
     9241 gaaatcggtc tggcgcatgg ccgccaggcc aacgctgcgc gcgtggtgca gcgcctcgcc
     9301 gaagtccagc gcctcggcat gcgtgtgggc cacggtggcg ccgtcgatgg cgctgctcag
     9361 cgcctgcgcg ggctgctggc cgatccagcg gccggcgatg aaactctgca aagtggtcat
     9421 ggcaagggct ccgttccaag ggtcggggcg gtggccggcc aggcctgtcg gcctggtcgc
     9481 cccacccgct gatgatgagt caaattgggc ttcagcgctt gtctggagag cgccgacagc
     9541 tatgctttcg atagtattcg agcgcgatca ggcggccgcg cgggcgccgc ccacacccag
     9601 gtaggtgtcg atcacgcgct ggtcgtgcat cagctcgccg gcgtcgccct gcaccgccac
     9661 ctcgcccatc tccagcacgt agccgcgatc ggccacgttg agcgcggcgc gcgcattctg
     9721 ctccaccagc agcaccgaca cgccggtgcg ccgcagcgcc gacaccgtgt gcagcacctc
     9781 gcgcacgatc agcggcgcca ggccgagcga gggctcgtcc agcatcaaca ggcgcggccg
     9841 gctcatcagg gcgcgaccga tggccagcat ctggcgctca ccgcccgaca gggtggacgc
     9901 catctgggca cgtcgctcct ccaggcgcgg gaagatgccg aagatctcct tcatgcgcgc
     9961 cgcctggtcg cgctggccac gccgccacag ggcaaagccg cccagcagca ggttgtcctc
    10021 gaccgacatc tcgccgaaca gctcgcgctt ttccggcacc agggccacgc cgtggcgcac
    10081 catgatctcc accgacgggc gcgccaccgc atgcccgccc aggttcagcc cgccctgcga
    10141 gggcagcagg cccatgatgg cgttgagcag cgtggtcttg ccggcgccgt tggggccgat
    10201 cacggtgacg atctcgccct cgcccacatg caactcgatg ccatggatgg cctggaccgg
    10261 gccgtagaag accgaaaact gcggcaggct cagcagtggc gcactcacag cacgtccccc
    10321 cccaggtagg ccgcgatcac gcgcgggttg gcctgtacct cggccggcgt gccgtgggcg
    10381 atcacggtgc cgaactccag caccgtgatg cggtcggcca ggttcatgac gaattccatg
    10441 tcgtgctcca ccaccaggat ggccagcccc tcgctgcgca actgcgtcag caacgtggcc
    10501 agaccctgct tttcgagatg ccgcagaccg gccgccggct cgtccagcag caggatggct
    10561 ggctgcccgg ccagcgcgcg cgcgatttcg acgatgcgct gctggcccag tggcagcgcc
    10621 gcggccgggg tgtgggccac ctcggccagg ccgcagcgct cgatctgccg catggcctcg
    10681 gccagcaccg ccgcctcttc ggcacggtcc agccgcagca tgctggccag ccagccctgg
    10741 cggccgcgcc ggtgcgcgcc cagagccacg ttttccagca cgctgcgctc gcccagcagg
    10801 cgcacatgct ggaaggtgcg gcccaggccc agcgcggcga aggcgcgcga gggctggccc
    10861 tgcatcacct gccccatcag gcgcacctgg ccttcggtcg gatcgtcgac gccggagatc
    10921 atgttgaaga aggtgctctt gccggcgccg ttggggccaa tcagcgcatg cacctcaccg
    10981 gcgcgcacat ccagatcgac cgcgttgttg gccagcaggc cggcaaagcg cttggtgacc
    11041 ttgtcggcct gcaggatcag ttcgcccgcc ggtggcagct ggcgctgcgc cagcgtggcc
    11101 gcgccggcgg ccagccgccg gggtgccgcc gctccggtga agcgctgcag ggcgccagcc
    11161 agcatcggcc acagcccgtt gggcgcgcgc tgcagcacca gcagcatcag caggccgaac
    11221 accacgattt cgaaattgcc gctgctgccc agcaggcgcg gcagccagtc ctgcagctgc
    11281 tccttcaaca gcgtgatcag ggtggcgccc agcaccgcac cccacaggtg accggagcca
    11341 ccgaccaccg ccatgaacag gtactcgatg ccgatgttga gcgcgaacgg cgtcgggttg
    11401 acgaagcgct gcagcagcac gtacagccag cccgacagcg ccgccagcag cgccgccagc
    11461 acgaacacct tgatgcgctg gcgcgccgtg tccacaccca tcgactcggc catcagccgc
    11521 ccgctcttga gcgcccggat ggcccggccc tcgcgcgaat ccagcaggtt gtgcaggccc
    11581 cagatggcca gcagcagcac cgcccagatc accaggccga gccgccgcgg cgaggccagc
    11641 gagacgccga acacctccag cggcgcgatg cccgacagcc cggtgaagcc gcccagcatc
    11701 tccaggttgc cgaacacgta gtacaggctc agcccccagg cgatggtgcc cagcggcagg
    11761 tagtggcccg acagccgcag cgtgacggcg cccagcaccc aggccaccgc cgccgtcacc
    11821 gccagtccca gcagcagccc gacccagggg taggcgccct tgggcagtgc ccccagccac
    11881 tgcgccgcct cgggcgacag gctgatccag gcggtggcgt aggcccccag gccgacgaag
    11941 gccgcctggc caaacgaggt catgccgccc acgccggtca gcatcaccag acccacggcc
    12001 accagcgcat acaggccgat gtagctcagc agcgtgacgg tgaacggtgg cagaaagccc
    12061 caggccgcgc ccagcaggac gatgaaggcc agaaccaggt gcatcggctt cattcctcgt
    12121 cctccacgtg gtgcgtgtgc agcgagcgcc accagagcac cggaatgatc agcgtgaaca
    12181 ccagcacttc cttgaacgcg ctggcccaga acgaggcaaa cgattccagc tgtcccacca
    12241 gcagcgcgcc ggccagcgcc agcgggtagc tggccagccc accgacgatg gcggccacga
    12301 agcccttcag gccgatcaga aagccggtgt cgtagtagat ggtggtcacc ggcgcgatca
    12361 gcaggccgga gacggcgccg atcagcgccg ccagcgcgaa gctcaggtca cccgacagcg
    12421 ccgtcgggat ccccatcagg cgcgcgccga cccggttgat ggcggtggcg cgcagcgcct
    12481 tgccgacgat ggtgcgctcg aagaacagga acatgcccac caccagcgcc gccgtcaccc
    12541 ccaggatcac cagcgactgg cccgacaccg tcaggcccag caggtcccag cgggcgtcgg
    12601 agaaggcctc ggtgcgggag ccctcggcgc cgaagaagaa cagtcccagc ccgaccagcg
    12661 ccaggtgcag cgccaccgac acgatcagca ggatcagcac cgtggcgtcg gccagcggcc
    12721 ggtacgccag ccggtacagc agcggcccca tgggagtgac cagcagcagc accgccagcg
    12781 agcgcagcag catggcctgc ggcttgagca ccagcagcat cagcgccgcc agcagcggca
    12841 gcagcacggc ccagaacagc gtgctgcgcc agtccacctc ggcgccgcgc gtctggcgca
    12901 ccagctcgac gatcagcacc agcgccgcca gccccagcag catccacagc agcggcggcg
    12961 actgaccggc ctgcagcgcc gccatcgcca gcgcggcgta ggccacgaac tcaccctgcg
    13021 gaatgaagat gacgcgggtg accgagaaca ccaacaccag cgccagcgcc atcagggcat
    13081 aaatggcgcc attgatgatg ccgtcctgca ccaacatcac ggcgatctga agatccatgc
    13141 gagcggcgcc gggcaagcgg cgcattcggt tgaaaacgtg cgcggcgacc ctcggtcgcc
    13201 gcgccgtggc ctgaaggccc cgggtttact tgacttcctg gtacttccag cggccgtttt
    13261 cgatcttcac catcacgcgg gcgcgctggt ccagacccag gtggtcggag gccgacatgt
    13321 tgaagatgcc atgcgcgccg ggcacctcct tgacgccttc cagggcatcg cgcagcgcgt
    13381 tgcggaactg cggcgtgccg ggctggccgc ccttgagcgc caccgggatg gccgccttca
    13441 ggatctggcc ggcgtcccag gcgtggccac cgaaggtgga cacgctgccc ttgccgttgg
    13501 cgccctcata ggcgttgatg tattccagcg ccgacttgcg caccgggttg gcggccggca
    13561 gctggtcggc caccagcacc gggccggccg gcagcaacgt gccttccacg tccttgccgc
    13621 ccacacgcag gaagtcgttg ttggccacgc cgtgggtctg atagtacttg ccggtgtagc
    13681 cgcgctcctt gagcgttttc tgcggcagcg ccgagggcgt gccggaaccg gccaccagca
    13741 cggcgtccgg cttggcgccg atcagcttca gcacctggcc ggtgacggcg gtgtcggtgc
    13801 gcgagaagcg ctcgttggcc accagcttga tcttcttcag gtccagcgcc ttgaccgtct
    13861 cgcggtacca gccctcgcca taggcgtcgg aaaagccgat gaagccgacc gtcttgacgc
    13921 cgttggcggc catgtgctcg gcgatcgcca gcgccatcat gatgtcgttc tgcggcgtct
    13981 tgaaggccca gcgcttcttg ccgtcctgcg gctcgatgat ggcggccgag gcgccgagcg
    14041 aaatcatggg cgtctgcgct tcggccacga cgtcgatcat ggccagtgac gtgggcgtgg
    14101 tggacgagcc gatgatgacg tcgacgttgc gctcggtgat cagcttgcgc gtgttggtca
    14161 ccgtctgggt ggtgtcggag gcgtcatcga gcacgatgta gttgatcttc tgcccgccga
    14221 tggtctgcgg cagcagcgcg atggtgttct tctcggggat gcccagcgaa gcggccggcc
    14281 cggtggccga caaggtgacg ccgacgttga tgtcggccca ggcggcggtg cagccgaagg
    14341 tgatggcggc agcaagcagc gccttcttgt ttgcgagcat gtgggtggtc tccaggagaa
    14401 agttgaacgg cgggcatcga ctcggcccga gcccaataaa cagaccgatc ggtcggcgaa
    14461 tgattctacg tgcgactgac gcaggtcaaa gccatgctct cgtcggtgat ttccctagcc
    14521 gcgcggcgcg ccgcagcgtc cccacgcggc cgaccgaggc gcccaacaac cccgcagcct
    14581 gggctgcggc gcgcatgggc agcgcccagc cggtgagccg gagcggccga gcgggcacgc
    14641 ggggtcgttc acacctgctc agcccagcac atgggccagt gtcagcagca ccagcaacag
    14701 cagccacagc gccacggtgc gccacaccag gcccaccacc tgggtgaaat gggccgtgcg
    14761 cggcggctcg ccgggcagcc cctcgcccga cacgccggcc gcgctgaccg gcaccgccgc
    14821 ggcgccctcg ggtgtcagcg gccgcagcga ggcaccgccc agtcgcaccc ccagcgcacc
    14881 cgccgtggcc gccagaatga cgccgtcgtt gcgatcggca aagcgcgacg cgtggctgcg
    14941 ccagacttcg atcgcgtcct cgaagctgcc gacgatggca aatcccaggg cggtggtgcg
    15001 tgccggcagc cagttgatga gctgccatgc ctgctcggcc gccaggcaca gcgccgggct
    15061 gctgggctgg tcggccgcct gcgccttgcg gcgccagtaa cgcgccacgt actcggcgct
    15121 gcggaagaac accgccccgg ccggccccag ccccagcagc gctccgatgc agaaccaggc
    15181 cagcacaccg aacacatggc gatgcgcggc cagcaccgaa acctcgatca catggcgcac
    15241 gatctcgctg cgcggcaccg tgcttgcgtc cacgtgctgc cagtgcgcca gcaggccgcg
    15301 ggcgcgctcc tcgtcgccgg cttccagggc gtcccggatg ccggtgaaat ggtggctgaa
    15361 ctgccgaaaa cccaacgtca ggtagagcat ggccacgctc cagaccagcg ccagcggcca
    15421 gccgcccagc cacgacagca gcaggtgcac ggcgctcacc gccagcgccg gcacggccac
    15481 cgccagcacc caggccagcc agccatgctg ggccgcgccg gcgtccacgt tgcgcccgac
    15541 caggcgcacc cagcggtgca ggccgaccgc tgccggatgg tccgacgtca ggggacgcac
    15601 ctgctccagc agcagcgcca gcgcgatcgc aaaaaaggcc atggcccgat gatagtgagc
    15661 caccgacagg tgccagaggc agggcggctc aggcggatag aaatctgtag aagttgcgca
    15721 acatgccggc ggtggcaccc cagatgaagc gctccaccgc tgcgcccggc agcgactcgc
    15781 cactgcccgc caccacgctc aggggctcca cgtagggcat ggaaaaccac tcccgcgcct
    15841 gcccctccca ggtcaggcga tggcgtcggt ggtgggccgg attcatcagc cacgccagcg
    15901 gcacctcgaa ggcatgggcc acctcgcgcg ggttggggcg cagcacgaag ccgggctgaa
    15961 ccaccgccac caccggcgtg acgatgaact gcgtaccggt cacgtacacc ggcagttgcc
    16021 ccagcacgtc gacgaagcgg cgcgacagat cgacctcctc ttcggcttcg cgcagcgccg
    16081 ccgccaccgc gtcggcatcc tccggatcga ccttgccacc gggaaaggcc acctgaccgg
    16141 aatgggtgga aaggttcccc gaacgctcgg tcagcagcac cgacagcccc tgcggccggt
    16201 ccaccagcgg caccagcacg gccgccgcgg ccggtacgcg gtcggtgaag cgcggctcct
    16261 ggcgcagttc gggctgccac ggcggcggcg cctggaagcg ccggcgcagc gcaggcagcg
    16321 tcagcgcctc ggccggcacc ggcggcagat gggcatcgat tcccgtcacc ggcacctggc
    16381 gcggatcgaa gttgggcagc ttggacagcg gggtgggcgt actcatggcg cacatgccgg
    16441 ccagctcgcc agcggtcgtc cggacatgaa aaaaccgcct gagccagggg ccgcaggcgg
    16501 ttccgattgt gagcgcaaag ctgccgatca gtcggcggcg gtcggcgaag ccgcgcggcc
    16561 ggggagcttt tccttgatgc gcgccttctt gccgctcagg ccgcgcaggt agtacagctt
    16621 ggcacggcgc acatcgccgc ggcgcttgac ttcgatcttg gcgatcagcg ggctgtaggt
    16681 ctggaaggtg cgctcgacgc cctcgccgtt ggagatcttg cgcaccgtga agccactgtt
    16741 caggccgcgg ttgcgcttgg caatcaccac gccttcgtag gcttgcacgc gcttgcggtt
    16801 gccttcgacg acgttgacgc tgacgatcac ggtgtcgccg ggggcgaact cggggatgct
    16861 tttgccgagg cgctcgatct cttccttttc gagctgctgg atcaggttca tggtttcctc
    16921 atctctcgat cgtgcgcacg ctacgctagg ggcggccaca ctgaacatgc atccagcgtg
    16981 ttgaatctgt gccgcggctg gcagaggatc gaaaagcctt tgattatagc ctgaccgtcg
    17041 ccttcaggcc atcaccctgg tccgccgcgc ccaagctggg ccagcagcac ctcgtcgtgc
    17101 gcgctcaggc gcccggcggc gcgcgcggcc acgatcaggt ccgggcggtg gcgcgccgtc
    17161 aggcgcaagc gctgctcgcg ccgccagccc tcgatgcgcg catggtgtcc cgacagcagc
    17221 tcggacggca ccggcacccc gtcccagacc tcgggccgcg tgtagtgcgg gcagtccagc
    17281 aggccatcga cggccaggtt gaaactgtcc tggtggtggc tgccctcatc gcccagcacc
    17341 cccggctgca ggcgcgccac cgcgtccagc agcgccagtg ccgcgatttc gccgcccgac
    17401 agcacgaaat cgcccaagct gatctggtgc gtcacatggc ggtcgatgaa gcgctggtcg
    17461 atgccctcgt agcggccgca gatcagcacg gcgccggcac cggcggccca gcgctcgacg
    17521 ccggcgtgtg tcagggtctg gccgatgggc gaaaacagca ccacgggggc ggcgccgccc
    17581 gcgcgctcgg cctgcaccgc cgccaggcag cgcgccagcg gctcggccag catcaccatg
    17641 ccggggccac cgccgaaggg acggtcgtcg acgcggcggt agttgccctc ggcgtacgcg
    17701 cgcgggttcc acagtttcag gacgatctgg ccactggcgt aagcccggcg ggcgacacca
    17761 gcttccagaa acggagcaaa cagttcagga aacagggtga tgacgtcaaa gcgcagcgcg
    17821 ccgccggcgc ggtcgctcac tgcaccgacc ccggttgcgg cgactgctgg gccgccgggg
    17881 tgaacaggcg cgccacatcg acggcgtcga agcgctgctg ctgaccgcag aagtcgcagc
    17941 cgacctcgat ttcgccgcgt tcggccagga tcgattcggc ctcgggttgg cccagcccct
    18001 gcagcatcgc cgccactcgc tcgcggctgc acgagcacgc aaagcgcggc ccctggtggc
    18061 cggccagcgg ggcgaagcgc agcagcggct cctgccagaa caggcggtgc aacagggtgt
    18121 cggcatccag gctcagcagc tcggcgcgct gcaggctgtg cgccagcagg cggatgcgtt
    18181 gatagtcgtc gtcggccgcg ctgccggcgt cctcgcgcgc cacgctgctt tgcgacaggt
    18241 tgtcctggcc ctgcaccggc aggcgctgga tcagcaaacc ggcggccacc tgctcgtcgg
    18301 ccgccagcac cagcgtggtg tcgagctgtt cggactggcg catgtagtgc tccagcacgc
    18361 tggccagcgt ctgcaacggc gcaccgccct cgtccaccag tggcaccacg ccctggtagg
    18421 cctgctggcc ttcgcgccgg cgacgcggat cgagcgtgat ggcacagcgg ccgcggccgt
    18481 ggcgattgac cagctcgggc agcgccatgc ccggtgtcac gctctgcacc accgaggcgg
    18541 tggcgcgcag gcgcaggtcg ggctgcacct cggccaccgc cagcttgagc gggccgtcgc
    18601 cgaagatctg catgatcagc gcgccgtcga acttgatgtt gccctgcatc agcacggtgg
    18661 ccgcggccat ctcgcccagc atttcggcca ccaccggtgg ccaggcgccg gtctgggtgt
    18721 tggcggcgcg ccggcgcagc acctcctgcc aggcgtcggt caggcgcacc agctggccgc
    18781 gcaccggcag gccgtcgaag atgaatttgt gcagttcgga catcggaggc agatggggct
    18841 tcagccaagc cgcttcaagc cagcctgaaa gcgccgcgcg ttgtcgacgt agtggcgcgc
    18901 gctctggcgc aggccctcga tctgctcggg cgtgagctgg cgcaccaccc gcgccggcgc
    18961 gccgacgatc atgctgccgt ccggaaattc cttgccctcg gtcaccagcg caccggcgcc
    19021 gaccaggcag ttcttgccga ttcgggcgcc gttgagcacc accgcgccga tgccgatcag
    19081 cgactcgtcg ccgacggtgc agccgtgcag catcaccttg tggccgacgg tgacgtggcg
    19141 gccgacggtc agcggctggc cgacgtcggc atgcagcacg ctcaggtcct ggatgttgct
    19201 gccctcgccg atcatcatgc gttcggtgtc gccgcgcagc acgcagccga accacacgct
    19261 ggcgccggcg cccagactga cgttgccgat cacctcggcc gaatcggcga tccaggccgc
    19321 cggatccacc tgcggcgcca cgccgtccaa ctcatacacc gccatcgctg gtctccttcg
    19381 ctcgctgctg cgcgaaacat agaattctac gaatggacct gcgccccctg gccctggcgg
    19441 ccctttgcga agccgatgtg gaacgaaaac tggctctggt ggccgccctg caagcgccgg
    19501 cagctacgct ttacatagcg cccgagaccg ctctcgaggc gccacccggc ctgcccggtc
    19561 gtcccttgcg gccgccgctg atcgatccgg cgcgcgtacc gcagcgcagc gtgcacacgc
    19621 gcgagggccg tgcggcgctg gtgcacgcca tctgccacat cgaattcaac gccatcaacc
    19681 tggcgctgga cgccatctgg cgctttgccg gcatgcccga ggcgtattac cgcgactggt
    19741 tgcgcgtggc gatcgaggaa tcgcaccact tcagcctgct gcacgcccat ctgcagaccc
    19801 accaccagct gcgctacggc gacttcgagg cacacgacgg gctgtgggcg atgtgcgaaa
    19861 aaacccgtga cgacctcacc gcccgcatgg cgctggtgcc gcgcacgctg gaggcgcgcg
    19921 gcctggacgc cacgccgctg atccagcgca agctgcgcgc cgtggccacg cccgacgcgc
    19981 tggccgcctg cgacatcctc gacctgatcc tgcgcgagga aatcggccat gtggctatcg
    20041 gcaaccactg gtaccgctgg ctgtgcgcac gccagggcct ggagccgctg gcgcactacc
    20101 gccggctgac gcgccagcac cgcgccccgc gcctgcgcgg gccgttcaac accgaggccc
    20161 gtcgacaggc cggcttcagc gacgacgaac tgcgcgcgct gagcgccgac ccgccaaccc
    20221 ccagctgagg cggcgccaaa ccagccctgg cgcgcctgcc gtcagaccgg atcggtgccg
    20281 aagcgcttca tcagctcgcc ctgcaccgcc gccggcgaca acgcctgccc ggcccgctcg
    20341 cgcgcgaaca cggccgccgc cgccgggtcg gggtggaact tcaggtacag gcgccgcagc
    20401 tggtcctcgt ccgcgtggcc cagttcgacc tgctcgtcga tgcggccggc gcggatcagc
    20461 gccgggtcca gccgctccag gtggttggtg gtcatgaaca gcaacgtgcc ctcctgcgtg
    20521 gccacaccgt ccagcgcgtt cagaaagccg ctgaacgaca gtcgcacctg cgcgtgcgcg
    20581 gcctcgcgtt cgcgaaagaa cgcgtccacg tcctcgatca gcagcagcgc gcgctgcggc
    20641 accgccgcca gcagagtgtg gatcttctcg tcggtgacga cgggcgaggc caggctgagc
    20701 gtgcacacgt tcaggtgcag ctcgctggcc agcgccgtca ccagcgaggt cttgccggtg
    20761 cccggcggcc cgtacagcag gtagccgcgc cgccacggaa tgcccagctc ggcatagcgc
    20821 tcgcggctgg ccagaaagcg ccgcaggtcg gccagcagcg cctcggcctg cccggccttc
    20881 agcaccaccg aggccagcgg ccggcgccag cccagtcgcg cgcgcatcca gtcgccaccg
    20941 tgaaaggggt tgggcaggta gaccgacagc cggtcggtct cgcgcgccgc gcgggcgtcg
    21001 atggcggctt gcagcaggtc ctgcaggtag cgcggcgaac gccccagcgt cgacaggctg
    21061 aggcgctcga acacgctctg gcccacctgc agttcgcgcc gcagccacag ccagcggccg
    21121 tccacgcgca ggatgtgcac gccttcgccc gggctcagaa aggcgcgcgg cggctgaccg
    21181 gcgcgcaggt cctcggccag gctctggtgc tcggtgccgc ggcgcacgct gcgcgcggtg
    21241 aactggttga cgcccgccag gcccgggtgc tgatccatgt gctcgaccag cgccggaaac
    21301 aggaactcgt cgcgcgaatc caccgtcagg gtgctgacga agaagcgccg gccccattgc
    21361 agcagcagcc gcggcaggtc cttgagccag tacgccagcg cgccggccgc agccagcagc
    21421 gccgcttgca gcgcgggttg ggacagcagg ccgaccgggc tttccatggg cgcgctcgca
    21481 tcagatgaaa gcccgatcgt accccagagc ttgccgatcg ggcgccagca gctatcgctt
    21541 taggagtccg cccggcccgc cccgacgccg cccgcgccag tccaccaccc acgcacggca
    21601 ggcttttgct acgctgccat ccattgccca ccccgacgtc cgccatgcac cgctcacccg
    21661 ccctggaccg cgccctgcgc cccaccctgc tcgtcctgtg cggcctggcc gccagcgtcg
    21721 gcgcgcacgc cctcggcatc cgctcgctca gcccgcaggg cgaggtggcg cgcgtgaacc
    21781 aggtggtggc cagtttcgac caggacgcgg tgcgcatggg cgatgccggc gcgccggcgc
    21841 cgctgcgggt ggactgcgac caggccgccg ccgcgcgcgg ccaggggcgc tggcgcagcg
    21901 cgcgcgagtg ggtgtttcag ttcgaggccg acctgccgcc cggcgtgcgc tgccgcgtca
    21961 ccccggagcc cgggttccag tcgcccaccg gcgccccatg gacgggggcc cggagctatt
    22021 cattccacac tggcgggccg ttcgtgcaac agctggtgcc ggccccgacg tcgaccatcg
    22081 aggaagacca ggccttcgtg ctgcggctca atggcgaagc cagcgccgac agcctgaacg
    22141 cccacctgtg gtgcgccgtc gacggcctgg gcgagcgcgt gccggtgcgg ctggtcgaag
    22201 gcgcgccgcg cgccgcgctg ctcaagcact tgggctggga caaggcggcg cagcagaccc
    22261 cgggccgcta cgtcgcgctg cagtgcaacc gccgcctgag cgccgccgcg cgcgtgcagc
    22321 tggtgttcgg cgccggcgtg gccacgccca gcggcgtgct caaccgcgtc gagcggcgct
    22381 ttgccttcac cgtgcgcgaa ccgttcagcg ccagcctcag ctgcgagcgc gaaaacgccc
    22441 aggccgcctg catgccgatc cgtccgctga cgctgaagtt cagcgccccg gtcacgcgcg
    22501 cccaggctgc acaggcgcgg ctgcgcgccg acaagctgga gtacgagccg gtgtttgacg
    22561 agccgcagga cggcgacgcg ctgatcgagc aactgcgctt tcccgccccg ctgcccgagc
    22621 gccgcagttt caccctgcac ctgccggccg atctgaagga cgccagcggc cgcgccctga
    22681 ccaatgccag cagcttcccg ctgcgggtgt ccaccggccc gctgcccccg ctggccaagt
    22741 tcgccgccgc gccgttcggc gtcatcgaac gctttgccga aggccccgat ggcccggccc
    22801 tgctgccgct gacgctgcgc cgcgtggaac cggcgttgca ggcgcaggcc ctgcacatcc
    22861 acagcctgca accgcaaagc gacgccgaca tcattgcctg gttcaagcgc gtgcagcgct
    22921 acgacgaggc ctacgtgccg cgcgcgctgg cccggcgcga cctgggtgaa cggctgccgc
    22981 cgccggtcaa cgagcagaca cgcgatcagg tcgagtcgcg cacgctgtcg ctgctgcagg
    23041 gccgcagcgg gctcaacacc ttgacgctgc cggccgcgcc caaggaaggc gagcgcccgt
    23101 tcgaggtggt tggcgtgccg ctgccacccg gctttcatgt gctggaactg gcctcgcagc
    23161 agctgggcga agcgctgctg gacgccaggt acggtgcccg gcgcccgatg ttcgtgcgca
    23221 gctcgaccct ggtgaccaac ctggcggtgc atttcaagct cgggcgcgcc ggctcgctgg
    23281 cctgggtgac ggcgctggac agcggcaagc cggtggccga cgccctggtg cgcgtgtccg
    23341 attgccacgg caagccagcg gcggaggcgc gcaccgacgc ccaaggcatc gcccgcttcg
    23401 atcgcctgtc gcccgagccg cccagctgct cgggcgagga cgagttctac gccgactttc
    23461 agcaggcctg gttcgtcagc gcgcgtgccc ggcaggccgg tgtcgacgag ctggccttca
    23521 cctggtcgtc ctggcagcgc ggcatcgaac cgtggcgctt caacgtaccc accagcagcc
    23581 agccgcagcc cgacctgcgc gcccacacgg tgttcgaccg cgcgctgctg cgcaccggtg
    23641 aaacggtgtc gatgaagcac ctgctgcgca gcgagacgcc tggcggcttc ggcctgccgc
    23701 cgcagatgcc ggccacgctg gtcatcaccc acctgggcag tggacaaacg ttcacgcagc
    23761 cgctgcagtg gcgccgcacc gccaccggcg ggcgcagcgc cgaaagcagc ttcgccatcc
    23821 cgcaggcggc caaactgggc gtctatcagg tgacgctgcg ccacggcggc gcagcgggtg
    23881 cggacggcgg cgacgacgcc ggcgacatcg aaaccgggag ctttcgcgtc gaggagtttc
    23941 gcctgccggt gctgcagggc cgcgtcgccc cgcagtcggg cgagccgctg gtggccgcca
    24001 cccaggtgcc ggtgcaggtg caggtcagct acgtctccgg cgggccggcg gcgcggctgc
    24061 cggtgcgggt gtcggcgctg acgcgcggca agacgctgtc ctttgccgac tacgaggcct
    24121 tcagcttcga gccgccgcgc ccgcccgccg ccgggcccga ccgcagcgac gacgccgaac
    24181 cgccggacgc ggcacgccag gaccagcagg tggtggccga caagctggcc gtcacgctcg
    24241 acgcgcaggg cagcggccag acccgcatcg aagccatcgc cgccgcgccg caggcacgcg
    24301 agctggtgct ggaagccagc tacgccgacc ccaacggcga aatccagacc ctgcgcggca
    24361 gcaccacgct gtggccggcc ggcgtgatcg ccggcatccg tgccgacagc tggacctcgg
    24421 tgcagcagac gctcaagatg caggcgctgg cgctcgacct ggacggccag ccacgcgccg
    24481 gcgtgccgct ggacgtcaag gccgtggcgc gcagcaccac cagcagccgc aagcgcatgg
    24541 tgggcggctt ctacacctac gacaaccgca ccgaaacgcg cgagctgggc acgctctgca
    24601 ccggccgcag cgacacgcgc ggcctgctga cctgcgacat ccggctgacg cacccgggcg
    24661 aggtcgagct ggtggtcagc gcgcgcgacc cccagggccg cgccagccag gccgcgcgca
    24721 gcgtgtgggt gacgcgccag ggcgagctgt ggttcggcgg cgaggaccac gaccgcatcg
    24781 acgtgctgcc cgaaaagcgc gagtacgcgg tcggcgacac cgcgcgcttt caggtgcgca
    24841 tgccgtttcg gcgcgccacc gccctggtgg cggtggagcg cgagggcgtg atgcagaccc
    24901 aggtggtgga gcttcgcggc gacgacccga cgctgacgct gaaggtcggg ccggactggt
    24961 cgcccaacgt ctacgtcagc gtgctggcgc tgcgcgggcg gctggtcgag gtgccctggt
    25021 acagcttctt cacctggggc taccggcgcc cgcgcgactg gtggcaggcg ttctggcacg
    25081 agcgcggcga ctacgccccg cccaccgcgc tggtggacct gagcaagccc gccttccgtc
    25141 tgggcatggc cgagatccgc gtcggcaccg acgcccaccg catcgccgtc gacgtccagg
    25201 ccgaccagac cagctacccg gtgcgcggca aggcgcgcgt gcgcatccag gcgcgcctgc
    25261 cgggcggtca gccggccgcc cacgccgagg tggcggtggc ggcggtggac gaggcgctgc
    25321 tggagctgct gcccaaccgc agctgggacg tgctgccggc catgctgcgg cgccgcgcct
    25381 ggggcgtgca gaccgccacc gcgcagatgg agatcgtcgg ccgccgccac tacggtcgca
    25441 aggcggtgcc cgccggcggc gatggcggca ccgcgcccac gcgcgagctg ctggacacgc
    25501 tgctgctgtg gcagccgcgc gtggtgctcg acgaccaggg ccgcgccgac atcgaggtac
    25561 cgctgaacga cgccctgagc accttccgcg tggtggccgt ggccgacagc ggcacggccc
    25621 tgttcggcag cggccacacc cgtctgcgcg tgacccagga cctgcagatc atcggcggcc
    25681 tgccgccgct ggtgcgcggc ggcgaccggt accgtgccct gctgacgctg cgcaacacca
    25741 ccacccggcc gatgcaggtg caggtcagcc cgcgcgccac cctggtcgag ctgaagccgc
    25801 agagcgtcga catcccggcc ggcgaggcgc gcgaggtggg ctgggacgtc accgccccgc
    25861 ccgagctggc gacgctgcgc agcggcgaga tcctgtggga agtcgaggcc cgtgaccgcg
    25921 cgagcggcgc gcgcgacgcg ctcaagctca gccagcgcgt gctgccggcg gtgcccgtga
    25981 cggtgcagca ggccacgctg acgcagctcg aaggcccgct gacgctgcag ctggccgcgc
    26041 ccgccgcggc cctgcccggg cgcggtggcg tcgggctggc gctgcagccg acgctggccg
    26101 atggcctgcc cggggtgcgc gactggctgg cgcgctatcc ctacgcctgc ctggagcagc
    26161 agaccagcgt cgccctcggc ctgcgcgatg cgacgcgctg gcagaagacc gccgcccagc
    26221 tgccggtcta cctggacgcc gacggcctgg cgcattactt cccgccgcgc gccggtgacg
    26281 agcaccgcgg cagcgacacc ctcaccgcct ggctgctggc ggccacgcac gaggcgtcgc
    26341 gcctggacgc ggcctttgcc ctgccggccg cgttgcgcgc gcagatggtg gccggcctga
    26401 cccagttcgt cgaaggccgc atcgagcgcc ggcactggtc gccacgcggc gacctggacg
    26461 tgcgcaagct ggccgcactg gaagcgctgt cgcgccatgg cgccgtgcgc ccggccatgg
    26521 tcagcagcct gaccatcgcg ccccagcaat ggcccaccag cgccgtgatc gactggatca
    26581 acctgctgcg gcgcgtgccc ggcatcgccg accagcgcac gcggctgcag gaggcccagc
    26641 aggtgctgcg cgcgcgcctg tccttccagg gcacgcgcct ggcgttcagc accgagcagg
    26701 acgaccactg gtggtggctg atggcaggcg gcgacgtcaa cgccgcgcgc ctgctgctga
    26761 gcgcgctgga cgacccggcc tggcaggccg atctgggccg cctggccagc ggcctgatcg
    26821 cgcgccagca gagcggcgcc tggaccacca ccaccgccaa cctgtggggc agcctggcgc
    26881 tggagcaact ggctcgccag cgcgaacgcg aggccgtggc cggcaccacc caggccgccc
    26941 tgggcgacgc gcgggacaag gtcgactggt ccaaggtggt gcgcctgaag gccgacgagc
    27001 cgggcagcgc cgcgcacgcg cgcagcctgt tcggtgcccc cgccagcccc ggccagtgga
    27061 ccggcaaccg cctgctgctg ccctggcccg aaccgcccgg cgccccggcg ccgctgaccg
    27121 tgacgcacca gggcagcggc aagccctggc tgacgctgca agcgctggcc gcggtgccgc
    27181 tcgccgcccc ggtggccgat ggctaccaga tcacgcgcag cgtgcatgcc gtcgagcagg
    27241 ccaaccctgc gctgccggcg ggccagtact cgcgcggcga cgtgctgcgc gtgacgctgg
    27301 acgtgcaggc gcgcgccgac atgacctggg tcgccatcac cgacccggtg ccggccggcg
    27361 ccaccgtcct gggcagtggc ctggggcgcg attccgacct tgccacccag ggtgagcggc
    27421 gcagcggcca gggctggctg gcctatgagg aacgcagctt cgaggccttc cgcgcctact
    27481 acgagtttct gcccaagggc aagctcagcc tgagctacac cgtgcgtctg aacaaccccg
    27541 gtcggttcca gttgccgccc agccgggtgg aggcgctgta cgcccccgaa atgcacggcc
    27601 tgacgcccaa cgcaccgctc atcgtgcact gagcccaggg tgaatcgagg gcctcagaat
    27661 gctatcttta agatagcttc tggcgcttgc cggataagcg ctagcgacat cttttgccag
    27721 ggttcaaccc attgctccgg ctgcccgaag ttgacaggcg gtcaactgcc gtttccgggt
    27781 tcaataggtt cagtgaaccg agacgctggc ctcggcttcc tgcagggtgc gccacatcac
    27841 cttgccggtg ccgctcttgg gcagggcgtc gacgaactgc accgcgcgcg gcgccttgta
    27901 ggcggccatg ttggcgtggc accactcgac gatgtcctgc tcgctgacct gccccttgtg
    27961 gctggcgcgc agcaccacca ccgccttgac cgactcgccc cggtaggcgt ccttgaagcc
    28021 gatcacgcag gcttcctgga tcgccgggtg cttgaacatc agcgcctcga cctcggccgg
    28081 ccagaccttg aagccgctgg cgttgatcat gcgcttcagg cgatcggtga tgaagaagta
    28141 gccctcctcg tcgacgcggc ccagatcgcc ggtgcggaag aagcgcttgc cgtccagctc
    28201 gaagaacgcc gctgccgtgg cctcggggcg cttccagtag ccggcgaaca cctcggggcc
    28261 gcacaccacg atctcgccgg tctcgccctg cggcagttcc ttgagcgtct cggggtcgac
    28321 cacgcgcgcg tcggtgctca tgaacgggat gcccaggcat tgctgcttgg gccggtcggg
    28381 cgggttgctg tgcgagggcg cggcggtttc ggtcaggcca tagccttcgg cgtacttcag
    28441 gccgtactgc tcttccagcc gctgggccac cgccgccggc atggcggcgc cgccgccgcc
    28501 gatggtgcgc aggctggaca ggtcgaagct ggcgaagttg gggctggcca gcaggtcgat
    28561 gatcatggtc ggaatgttcg tccagcccgt cacctgccag cgcgaaatca ggcgcccggc
    28621 cagttcgcgg tcccagcgcg gcatcagcac catggtggct gcgctgtaga cggccgagtg
    28681 catcaccgac accatgccgg tgatgtggaa catggggacc accaccagca ccaccgattc
    28741 cgaatgcccc gtgccccaca ggctggaggc caccgcgttg tgcatgatcg agccatgcgt
    28801 gtgcatgcag cccttgggca ggccggtggt gccgctggtg tagggcagca ccgacaaatc
    28861 ctgcgggccg accgtggttt cggccagcgg gccaccgaag gccagcgcgt cgcgccaggt
    28921 gtgcacctgg ccgccggcga gctggggcag cgcgcgcgcg gtcagcagcc agtcgcgcca
    28981 gtgctcgggc ggggcgtcgt cgccctgcac gccggcgtcg aaagcgtcgc tgtactgcgt
    29041 cacgatcagg tgcgccagcc gctgggctgc gggcagcgcg tcggaggcgg ccgccagctc
    29101 gcccgccaga tcggcgctgg tgatggccac ccgggcgtcg ggatcggtga tgtagtgctt
    29161 gagctcctcg gccttgttca tcgggttgat cggcaccacc accgcgtcgg cgcgcagaat
    29221 ggcaaagtgc gccacgatca gctgcggcgt gttttgcagc atcagcagca cgcgatcgcc
    29281 cttggccacg cccagcgcct gcaggcgcgc ggccaggcgc tcggcagaga gcttcagctg
    29341 cgaccagctg agcaccgtgc cgaagaacac ggtggcgagc ttgtcagggt agcgcagcgc
    29401 attgatggcc aggttctgcc acaacgaggt ggctggcggg gtcaggctgt agggcaggcg
    29461 cttgggccag aaagcatgat gggggcgcac ggatcggtct cctgtgtggc ctcgcgacga
    29521 ggtcagtcaa ggttgaactg ggcgcaacat acatcaaaca tcgacgtctg cgtagccggc
    29581 tgtctcccgg gcgaccttcg ccgttcggct gccatcgtcc gtgcgcgggc acgcggtgcg
    29641 ccgttgcggg tccgccgaac ccgagccagc gtgcccgtgc tgcaaggccc cgtcccggac
    29701 ggcctgcaag gcgcattcgt cgccgggtcc cggcactgaa cctagaaacg gccgttgacc
    29761 gcttgtcaac ttcgggcagg ccgcatccgt gcagggtctc gcggctgcga tgaccttcac
    29821 ctgctgggtg agagcgctac gcacccaccc gcgccgaggc ggccgctcaa ccgggccgct
    29881 tgacgcaacg caccttgcgc ggaatcgact ggcgttcact gccgccggtg acctccttca
    29941 cctcgccctt gggacagctg cgatcgtcca cgtaaaccac cttccgatgc ggcaggctgc
    30001 cctttggagg gtcctccgtc aggtactcca ctcgcggctg cgcctgggcg ggcaccccca
    30061 gcgaaaccca cagcgttgcg gcaaccaacc agacatgcat cgtcatctct cgtcctccgt
    30121 cgggccacgc atggcgaacg cgacccggcc agtcaccatc gtgcgcccgt cattgaagac
    30181 cggccgaact ccgggcgcac cccaggccct gatcaaagat acttgctttc ggtcccgatg
    30241 gccatccagt accggcgtcg atcggtcgga tgcagatgga cctggatgtg atctccgacc
    30301 gccagccggg ggcgcggatc ggcacgcagg atgtcgctct tgaacacggt gggggcggcg
    30361 gtgccggtgg gcgccacggc gagcgcctgc acgcgccagc ccttgccgcg gcggccgatc
    30421 ttgaccacct cggcctgtac cgcccggccg tgccgccgca accagtcggc gtcgcgcgcc
    30481 ttgcggtcgg ccagcaacgc atacaacagc gtatgcagcg tcagtatcag ggcaacgaaa
    30541 ccggcagccc acgcgaacat cgacgctctg gccgacgcgc ccgcaccacg ggccagatag
    30601 gcggtcagca gccaccccag gacactcagg atgccgaaca ccagggtgcc ggcagttttt
    30661 cgagtcatgg cagcgcccgg tgcacgcaca ggcccggtca gaagcgggcg acgccccacg
    30721 cgccgcgccg aggcagtggc cagctaccca tggcgtcggt cagacggcag cgtcgtcttc
    30781 ttcgccggtg cggatgcgca ccacccgttc caccggggtg acgaagatct tgccgtcacc
    30841 gatcttgccg gtgcgggcgg acttgacgat ggcctcgacg cagcgatcca cgtcggcatc
    30901 ggtcaccacc acctcgatca tcaccttggg caggaagtcc accacgtatt cggcgccacg
    30961 gtacagctcg gtatggccct tctggcgacc gaagcccttg acttcggtga ccgtcaggcc
    31021 ggtgacgccg acctcggcca gcgcctcgcg cacttcctcc agcttgaatg gtttgatgat
    31081 ggcggtgatc tgtttcatgg atgctcctga agcggtcaga ctcggtatct gttggtcata
    31141 ggataacgcc agtccttgcc aaaactgcgg cgggtgacgc gggtgccgac ggccgcctgc
    31201 tggcgcttgt attcgctggc cttgatgagc cgagtgacgc gctccacatc ggctgcggca
    31261 aagccctcgg ccagcagatc ggcgatgctg gcattgtcct cgacgtagcg ggccacgatg
    31321 gcgtccagca cctcgtaggg cggcaggctg tcctggtcgg tctggccctc gcgcagttcg
    31381 gcgctgggcg cgcgcgtgat gatgcgctcg ggcatcgggt ttgcgcccgt gccgtggggg
    31441 tcgtgcgcgt tgcgccagcg cgccagccgg taggcggtgg tcttggcaac gtccttgatc
    31501 ggcgcgaagc caccgcacat gtcgccgtac agcgtgcagt agccgaccgc gtattcgctc
    31561 ttgttgccgg tggtcaggat caggtggccg agcttgttgg acagcgccat cagcagcaca
    31621 ccgcgcacgc gcgcctggat gttttcttcc gtcacgtcct cgccgcggcc ggcaaacagc
    31681 ggcgccaggg tggcgcgcag ggtctcgaag gccggccgga tcgacagctc gtcgtgccgc
    31741 acgcccaggc gccgcgccat ctcgtgcgcg tcctccacgc tcatgccggc ggtgtagggg
    31801 ctgggcatca tcagggtgcg cacccgggcg gcacccaggg catcgaccgc cagtgccagc
    31861 accagggccg agtccagccc gccggacagc cccagcgcca cgccggcgaa gccgttcttg
    31921 tccacgtagt cgcgcagacc aagcaccagc gcctcccaca gctcggcctc cggcgtcggc
    31981 tcgggggcca tatctgcttt caattcgata gctgccgccg cacgactggc ctgcacgaag
    32041 aacagatttt ccttgaaact ggcggcacgg ccggcgagct gaccatcggc gccgatggcc
    32101 agcgagcggc catcgaacac cacctcgtcc tgcccgccca ccaggttggc gtacaccagc
    32161 ggaagaccgc tggcgcgagc gcggcgcgcc atcgccgcct cgcgctcggc gcccttgccc
    32221 tggtggtagg gcgaagcgtt gatgaccgcc agcaactcgg cgccggcctg gcgcgtggcg
    32281 gcggcgggtt catcgaacca ggcgtcttcg cagatcagca ggcccacccg cacgccatcg
    32341 accgtgaaca cgcaactgcc ctgtccgggg gtgaagtagc gccgctcgtc gaacacttcg
    32401 tagttgggca gcaggcgctt ggcgtaatgc gccacccgcc ggccttcgca cagcacgctg
    32461 gcggcattgg cgcagcgcgg cccgacgtcg tcgaccggcg ccaccgcgct cggatgcccc
    32521 accacgatgg tcagcccgcc caaccccgcc gtctcgcggg ccacccgatt cagggcatca
    32581 tcgcaggcgg cgatgaaagc cgggcgcagg aacaggtctt cggcgatgta accgctgatg
    32641 gccagctccg gcgtcagcag caggcgtgcg ccctgggcgt gggcggtgtg ggcagcgtcg
    32701 atgatctgcc tggcgttgcc cggcaggtcg cccaccacga ggttgagctg ggcgacgcag
    32761 atcttgagag acatgaatgc aggcaggtgg cagaccgggc gagtatcgta acgcggcgca
    32821 gcggccggcc gacgcggcgc ccgcgctgca ggtgctggac acgccggccg cgctcgacgc
    32881 cgcgcgctgg aacgccctgc tggcgcgtca gcccagcccc acgcccttca tgcgccacga
    32941 atacctgctg gcgctgcacg ccagcggcag cgccgtcccg cacaccgggt gggcgccgcg
    33001 cttcatgctg ctgtgggccg gcagcgagct gatcgccgcc tgtccgctgt acctgaaaag
    33061 ccattccttc ggcgaatacg tgttcgactg ggcctgggcc aacgcccacg ccgaacacgg
    33121 cctgccctac taccccaagg cggtggtggc cgtgcccttc acgcccgtgc ccggcacgcg
    33181 cctgctggcg cgcgaccagg cggcgcgcgc ggcgctgatc gccgccgtgc gcgactggtg
    33241 cgccgaccag cggctgtcgt cgctgcacct gctgttcggt gatgaagccg atctggccgc
    33301 ctgcgaaggc gccggcctga tgcggcgcag ccaggtgcag tttcactgga aaaatggagc
    33361 gcccccacgc tccgcggctg cgcctgctgc gctgcccccc gtgggggccc tcgccgcctt
    33421 ggggcggccc ggcggcggct cggacgcgcc ggcggagcgt ccgtttgccg atttcgacga
    33481 cttcctggcc gcgctgacgc aggacaagcg caagaagatc cgccaggagc ggcgccgcgt
    33541 ggcccaggcc ggggtgcgtt ttcgctgggc tgcgggcaac gacatcacgg ccgaggactg
    33601 ggccttcttc taccgctgct accggcgcac ctacctggag cacggcaacg cgccctacct
    33661 cacgccggcc ttctttgccc acatggcgcg ccacatggcg gacgtctggg tgctgttcac
    33721 ggccgagcgc gaaggccacc cgatcgctac cagtttgata gctgtcggcg cacgcccaga
    33781 aagcgctaga ggctcaaacg atgcctatgc cggagcggtg gcctacggcc gctactgggg
    33841 cgcgctggag cgggtggact gcctgcactt cgagctgtgc tactacgcgc cgctggactg
    33901 gtgcatcgca cacggcgtgg cgcgcttcga aggcggcgcc cagggtgaac acaagatggc
    33961 gcgcgccctg ctgccggtgg ccacgcacag cgcccactgg ctggcgcacc ccggctttgc
    34021 cgacgccgtg gcgcgctttc tggcgcgcga atcgcttgcc gtcgatggct atctgggcca
    34081 gttgggcgag cgctcgccgt tccggcgacc ggacggctga ggcgcgcgcg cgccgccgct
    34141 cattcgcggt cgcggcgcgg ggtgcgcacg cgccggctgt cggggcgcag cgccgccggg
    34201 gtcagcgcgc cgccgtcgtg cagctcgact tcctgcccgc agggcgcatc gagcagaccg
    34261 aacaccatgt gggcgcccgg gccgaagcgc tccggcacgc tcaggtgcag cggcagcgac
    34321 atgtccaccg gcgggttcgg gctttccgac aggatctgac ccttgggccc cagcacggtg
    34381 tagcaggcgg cctgcgcgcc cacggcggcg cccagcacgg cggcgccgat cgacagcctg
    34441 gcaatggaaa gtttcatggt ggtcccccga ggttgaacgg ccggcgtcgg caccggcccc
    34501 gctgacgctg agtgtatgcc gctcagcgcc cgacgctgct gcccggcacc cccagttgcg
    34561 ggatcaggcg gtgatagaaa cggatcgcgt tggcgtaatg cgtcacgccc agccgttcgt
    34621 cggtgccgtg aaagcgcggc aggtcgtcgc tgacggcgcg gatgggcgag aacttgaaca
    34681 catgccggct gatggcctcg tagtgcaccg aatcggtgcc cgccaccatc agccccggcg
    34741 ccaccagggc gtcgggaaac acctcgcgga ccgtgcgcgc cagcatctgg tagggggccg
    34801 aatcggtggg cgcgacctgc gaggcttcct tgccgccagg cagggcggtc acttcgaact
    34861 ggccttcggg caccacccga gcgagctgct ggcgcacgtg gcgcagcacc tgctcgcgcg
    34921 tttcgcccgg cagcatgcgg aagttgaccg tggcctcggc gcggccgggc agcacgttgt
    34981 ccttgttgcc ggcctgcagc atggtcagcg cggtggtggt gcgcagcatg gcgttggtgc
    35041 tggccccgcc ttccagctgc ttctgcacca gcgggccgaa cagccacagg ttggacagcg
    35101 ccacgcgcgc caggccgccc atctcgggcg ccagggtgcc gaacatctcg cccgccacgc
    35161 cgcggatgcc ggccggcatc ggcgcgtgct ccagccggtc caggccggcg ctcagcaggg
    35221 cgatggcgct gctgccgggc ctgggcggca tggacgagtg cccgggcggc gccgtcaccg
    35281 tgatgcgcac cgacagatag cccttttcgg ccacgccgat cagcgccgcc ggctgcttca
    35341 ggccgggcat cacgccgtcg agcaccagca gcccctcgtc cagcacgaag tcggcctgca
    35401 cgccgcgcga cttcagcagc tcggcgatgc gggccgcgcc acggtggcca ccgacctcct
    35461 cgtcggcgcc ataggccaga tggatggtgc gcggcggccg gtagccctcg gccagcaggc
    35521 gctcgaccgc ctccagctgc gccagcaggt tgcccttgtc gtcccaggag ccgcggcccc
    35581 agatgaaacc gtccttcacc agcccggcaa acggcggctg ctgccagtcg ccctcggtgc
    35641 cgggcgccac cggcaccacg tcctggtgcg ccagcagcac gatggcgttg gctttcgggt
    35701 cggcgccttc ccaggtgtag agcaggctca gctcgttgac gatttcgcgc ttgagcgtgg
    35761 cgtgtacgcg cggaaactgg gtctgcagca gcgcgtgcag ggcgcggaac tgatcggcgt
    35821 tgagctgcgc gtcgctggcc gaagacacgg tctgcagccg caccgcctgc gccagccgat
    35881 cggccaccgc gggctcgtcg atcgccagcg gcgccagcgg cgccacctgc agctggcgcg
    35941 acgactggcg ccaggtgttg cccgccaccc ccgccgccag cgccagcagc acggccagca
    36001 ggcccagcac gatcttcttc agcatcagcg gtctcccgga attcgtgagc gatggtaacc
    36061 gcagcgcggc gcacggcggc gccccattca gcggccgcgg ccgcaggcaa gatcaagccg
    36121 gctggcgctc gagcgctggc cgcagcgcgc cggccagcgc ctccagcagg cgggcgcgct
    36181 cggcatcgtc cagtgcgccg gcgacgccgc gctcccacgc cgggtggccg cgggcggcgc
    36241 tcagcgcgtc gatcagcgcc tgcccgtcgt cgcccagggc gccggcagct tcggcgccca
    36301 ggcgcagccg ctgctgcgcc gcatggccac gctgcgccag cgcgcgcgcc agcgccagca
    36361 ggcgctcggc cagcagccac agcaccgggt cttccaccgt cagctcctgc acgccatcca
    36421 gacggttctg cagcttgcgc cacagcggct gccagccgcc ggagatagcg ccacccaggg
    36481 tggcgcccag ggtggtggcg gcgcccagcg acagaccggc cagcgccacg tcggccaccg
    36541 cgcccatgcc ggcaccgatc atggcgccca ggcccaggcg cctgccggcc tgcttgagca
    36601 gctcgggatt gaacaggtcg tcttcccagc ggccgccggg cagcggcagc tcggtcagct
    36661 cggcgtcgtc ggcgccgaag gcgtacagcg tcagcagctc gtcgacggcg cggcgcgcgt
    36721 ggcggccagt ctgggcctga aagtcacgca cccaggcctg ctgctgctcg gggtcggcaa
    36781 acgcctcggc cgacaggctg cggcgctggg cggcaacgtc gatcagcgtg ctggcgatga
    36841 cgcggcaggc cgccaggcgg cgctcgcgct cggcctgcgc cagcgcttcg acgatgtcct
    36901 gcaactgcgc gcggcgcgcc ggcagcaggg tggccaggtc ggcgtacagg cgctgctcgg
    36961 cgccggtgaa gggcgccacc acgtcgaact cggcacgcgc gtgcagattg ctttcttcca
    37021 gcgcgcggtg ccagtcggcc aggcggctgg cgccgtcgcg cacgaagttg agcaccggca
    37081 tcaccggctt ggcgcagctg gccaggattt cgatttcggc gcggtgcttg ggcagcaccg
    37141 gctcgcgcgt gtcgatcacc agcatggcgc agccggcctg ctccagcagg gcgcgcagca
    37201 ccttggcctc ctgctcgaag ctggcgcgcg cctcgggccc ggccaggaag gcgcgcacgc
    37261 gctcggggcg cgaatcgccg ctctggcgcg ccagaaaatc cagcagcgcc accgaatctt
    37321 ccagccccgg cgtgtcgatg aagcgcaccg cctcgcgccc gtccagccgc agcgccaccg
    37381 cctcggcatg gcgcgtggtg ccggggcgat ccgacacctg gccaaagtcg acccggcgcg
    37441 tgagcgtgcg cagcagcgaa gtcttgcccg cgttggtatg gccgacgacg gcgatggtga
    37501 gcgcttcagt catggtgtgc ggcgtcgccg gatgctatcc attcgatagc tgcttgcgct
    37561 tgctccacaa gcgccacagc ctcaaaaccc tctgaatcca gccaggcgcg ccagcgccct
    37621 gccccgctcg acccacccgc ccccagcgcc agcagcgcca tgcggtgaca cagcgcctgc
    37681 gcctcgcgca cgaagcgcgc cgtcccgcgg tcggggctgg acgggccatg caccaccagc
    37741 agcagcgact cgggccggct ggcctgcagg cgcgccagcg cggcctgccg atcggcggtg
    37801 ctgccggcga tgcgctgggg tggctcgctg cctgcagaca aaccggtggg caagccgggc
    37861 agcggccagg gctgctcggg cggcagctcg aaacccagca ccgccagcga acccggcgcg
    37921 ccgggcgggc cggcacgcac ggcgtaggtt ggatgggcgc tgcgctgctc ggcgtcgatc
    37981 acctgcggcg gcggctccag cgcgtcgagc cgcgccacga tgcggcgcac gtacgggtcg
    38041 gccatgtccg gctgcgccag ccgcgcctgc ccggcgcgcc agcgccagcc gcacagcagc
    38101 gccagcagca cccgcggcag caggccatag gccaccacgc agccgatcag ccaccaggcc
    38161 caatcgctct gggcgatggc ggttgcaccc acggcggcat ggcccgcctg cgtcaccgcc
    38221 gccgcgtcgg gcacggcaaa gcccagcagc gccggcagcg cgcccgtgat ctgcacgaag
    38281 cgttggaaga acgacgcgct caggatggtg ctctcccagg tcagcgcgta ggcatgaaag
    38341 gcaaagccga acaccagcac cgcccccatg atgacaaagc ccagcgccca caccgcatgg
    38401 ctgatcgcgc ccgtcagcca caccagcagg ccgtggcggc ccaacaccgc gcccagcgca
    38461 cgcagcagcg tcagcgcatg cggcccgcgc tccagcggca ggcgcgccga cagccacagc
    38521 gccagccgcc ccagcggcgc cccgccgcgc cgtccggccg gcagcaacag gcccagcagc
    38581 cacagcacca gcgtcagcgc gtgcagcccc agcagactga cgaacgccgc caccgcgttg
    38641 atgctgcgcc cctggcccag caccgcccgc gccagcccca gatcggccag cgcgagcaac
    38701 agcgccagcc ccagcagcac ccccgcgccc agccgccgcc agcgcgccca ttcaaccggc
    38761 agccccagcc gctcggccag caactgcgag cgcgccagca cctgcgcacc ccgtccgccc
    38821 ggcatgcggt gcgcctgcgc cagcgcctgg gcgtcgtcca gcggaccggc gtcttcgatg
    38881 cgacgcacgg tttcgtgcag gactgctgcg gcgacgggcg tgctgggact ggtggatgtg
    38941 gtgacgcgca tgagggcgtg aagtgctgca aagcatgata gcgagggacc tgcaccgcaa
    39001 acggattggc cggcgcaacg accgacgccc atcgcccggt ccgatcacgc ttcagcgcat
    39061 gcgcactaac tgatccacct cgatcatcga ccgctgcccg actgcaccgt tgcgcgtatc
    39121 ctgcgcctgg tgtgcaaccg ctacgagtcc ccccaagccc gcgaaatcga acgcctctgg
    39181 cacgt
//
LOCUS       DAOOOG010000007        38809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_806_length_38809_cov_21.714481, whole genome shotgun sequence.
ACCESSION   DAOOOG010000007 DAOOOG010000000
VERSION     DAOOOG010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 38809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38809
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_806_length_38809_cov_21.714481"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PK242_01285"
     CDS             <1..296
                     /locus_tag="PK242_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOZ92690.1"
                     /translation="VDAFQGKEFDLVFLSVVRANDVVIPDQKEGEERERLLNSKYGHL
                     RLANRMNVAMSRQRKLLVAVGAKHMAEGPESEEGVPALAAFLKLCREEMAHGR"
     gene            286..1749
                     /locus_tag="PK242_01290"
     CDS             286..1749
                     /locus_tag="PK242_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92691.1"
                     /translation="MAADRYLRFDVRRPPGGRPLLWPVRVWKVLYPTNRALKLNLFQQ
                     AILGLARARCQDSSEMAELLGLDRELVAFIIATQLIPNGWMTTSGSVTSQGERVLEEA
                     QDASEEVRMGYAYQDAISGNWLPRFTEELPEIEAKRVDERGYPVFLRDLDSGKEDRPF
                     RLNHFREDALDTAALFDAFQRYRTDHDHAKQRDEELSARVRIESLSFVEDSAQPMWLW
                     TWIFPDEAGPQPWLVADPFGLQQAASWLRKPLQDVLPRNDGLARYIADAVGETRSNDL
                     SAEEWLRSLENQIDLTLLADYNWSRKVPIVEGYLASVLRRRSVLASQERSWQEDVTSL
                     LIETHNLAESVLQWLLKQFSVDVRRLPDKSQDNKWTKQIAVQTLQALPIAKLDDELVR
                     RLASQNLREIRNALIRGNSSLKALMFAALLSTIEHSDHPFLKLSADELRLTRLLDMAD
                     ARNKKAGHSGSERIDREEAMEHADFVIEWVQIFKGWY"
     gene            1634..4123
                     /locus_tag="PK242_01295"
     CDS             1634..4123
                     /locus_tag="PK242_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ92692.1"
                     /translation="MLATKKPGIQVARESIEKKLWNTLTLSSNGFKFLRAGIEMAKHN
                     KGNKAPVAQNANTAQSAVASAWQKATAGIGSLASLVQGDASAVVLPDLEKDQVSELDK
                     VEPEKKIELERLLADISDAAKRVRDLHEDAQKRSDDLARKEEAFSRRSDENKKRTEEI
                     NKLDEELKQRQVEIKKSESSLADKERELEKRELDARSGFAAQNVAALSDLKKEIVELE
                     SRKTEIQLGILQAEQKARDDESARASEVMEREAEIANKERALQQQQRRLDAEWKELER
                     ERVQIREDSLREASQEIARLTDARLRAEARLEKVYRDWTLTEQKLEQYREFSEALAGR
                     SAREILDELAAQKRRVTQLENQIANSQDDQLQFENEALRKLRDEQKAQLDEVHFDLAD
                     AKAQLHRLRLGVSDKENLEREKRALEKNNQILSARLNDLGKTVDDLTQSQQSEKPFPQ
                     MAWMDSASTADWIKDRKAKELSLPEQDVPDLKKFSVELQHRIAQAEEGAILHFSLEDI
                     QLLIAGLAMSQLHIFQGISGTGKTSLAKAFAKAVGGHCTDIAVQAGWRDRDDLLGHYN
                     AFEKRFYERDCLQGLYRAQTAAYKDRCNIILLDEMNLSRPEQYFAEFLSALEKNDPRD
                     RLISLSESQLPNAPALLVEGRRIRVPHNVWFVGTANHDETTNEFADKTYDRAHVMTLP
                     RHEAGFKVEPKPKASFSYGSLMERFDAAVTQNADEVSELLAELTTGPLTSILQDRFDL
                     GWGNRLERQAMRFVPVYMAAGGRKEDALDHLLASRVFRRGKVTGRYDATIDDLAAIEQ
                     ALTTVWKGWKSEPRRSINLLAEDRRRKERGA"
     gene            4120..5943
                     /locus_tag="PK242_01300"
     CDS             4120..5943
                     /locus_tag="PK242_01300"
                     /inference="COORDINATES: protein motif:HMM:NF021352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2357 domain-containing protein"
                     /protein_id="HOZ92693.1"
                     /translation="MMFLDRLTGGKLSRLPDALLPGRYCLLEPLLVNGHSRLQQGDLL
                     SDDGSGVIRTQDTESQVLAIDQSDDDQADCDLSAEAIISIAGKIAHAHDANVSPMLPA
                     EMAAQCALEELERDLAVVLRDGHLHSISDRPRRDLRYDDLVAPVARARRLATSALSHL
                     ASHSDCWQQRTLSGVQPRKVLARFSEDDYAIYENRLYKRLLDRLDRHLARRLARIRGV
                     NSRLERALEFQDSEQTHFRLRQDICRLWGESYLDDKTGTQLEAGKRALSDLESQLRVI
                     RGLKQRGLYSLVPPASVVPAQVHRTNILNHDPHYRHLPPLWEKLKDDREDRLLPPEER
                     LARQLQLQLAYVSYVGLVIRRALERYGLRRVDGNFVFSWAGRSFVLRRDVHDWVVTQS
                     QGSTLRIVPIAWFGASINISESLAPGRIVCWPGGPTSVASPQSLPVSPLDLYVVEKVG
                     KLIDEWMLRQLLQGHGRKLGPLPTPAKRLTEAWPEQFESISSTHVRLLAPVDAQKAAE
                     LKASLKGSANSQVEDAVWDAIEQIATLAELCGHQARFVRGQQQDFYCQCGTCQATWSL
                     KTTGKRRHFSMRPKGASAIPEDDGFLWSGRDWLEFDLDSEQ"
     gene            6342..6683
                     /locus_tag="PK242_01305"
     CDS             6342..6683
                     /locus_tag="PK242_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOZ92694.1"
                     /translation="MSYQIKELLLADGDSPFAQWFGSLEAVAAAKVRVAVSRMEQGNL
                     SNVEWFRGIGEYKIDWGPGLRIYLAKDGLKIIILIGGGTKKRQQQDIDQAVALWDDYK
                     RRKASTQKGGK"
     gene            6685..7005
                     /locus_tag="PK242_01310"
     CDS             6685..7005
                     /locus_tag="PK242_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOZ92695.1"
                     /translation="MALTRDFKETVAARVQNDPAFAQALLDEAITLFVNGEPESAKLI
                     LRDLVNATVGFEALAEEIHKPAKSLHRMLSQSGNPTMSNISAVFAAIKRALKVEVRTQ
                     VVMA"
     gene            complement(7008..7460)
                     /locus_tag="PK242_01315"
     CDS             complement(7008..7460)
                     /locus_tag="PK242_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92696.1"
                     /translation="MKSKVQALGGPIARERRDGGQVKLSTFIPLKIRRRGASKVVVRP
                     DGAVESPGKVASQIDQPLLVALTRAFYWQQLLDDGVVGSGSEIAQREGLHHSTVNELL
                     RLTLLEPAIIQTILAGQQPRCMSLLWFQRNPLPTDWMAQRAVVAGFDA"
     gene            complement(7457..8767)
                     /locus_tag="PK242_01320"
     CDS             complement(7457..8767)
                     /locus_tag="PK242_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOZ92697.1"
                     /translation="MYCRVSSDERLDQSFNSIDAQKEAGQAFIKSQSHEGWIAVADDY
                     DDGGFSGGNMERPALKRLLADIQLGKIDIVVVYKIDRLSRSLADFARMVDVFDRHRVS
                     FSAVTQQINSATSMGRLMLNVLLSFAQFEREVTGERIRDKIALAKAKGMWMGRPLPLG
                     YDVRDRLLMINEVEAALVRRIFDDFVTMRSATLMAKTYGAEGVLTKGGKPFTKQTIYK
                     MLHNRMYLGKIVHKGQSFPGQHQAIITQAQWDAAHALIATDGVERRRETNDRQREPVL
                     LRGLLFTSDGERLVPSYTKKKGKTYRYYSPVKHRRLGAWASQHGSLPAAPIEDLVTQQ
                     IVAALSAPHIVQAVWDRMQHIRPDLTEPQVVLPMRNLAGLWRELFPAEQCRLAKLLIE
                     RVVIADGGLEIIWRDQGWQELAGELLPGTIGAELQEWERQEVEA"
     gene            8767..9896
                     /locus_tag="PK242_01325"
                     /pseudo
     CDS             8767..9896
                     /locus_tag="PK242_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097922.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            9966..10337
                     /locus_tag="PK242_01330"
     CDS             9966..10337
                     /locus_tag="PK242_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOZ92698.1"
                     /translation="MTRVFKTRHFQRWMRKTELTDAVLCKAVQEMAAGLIDADLGGSV
                     VKKRVGLAGRGKRGGVRTLVATNKGNRWFFVFGFEKNERANVSDEELEGLQTIAADLL
                     ARTGPQLDEAVADGALQEICQ"
     gene            10334..10651
                     /locus_tag="PK242_01335"
     CDS             10334..10651
                     /locus_tag="PK242_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOZ92699.1"
                     /translation="MTTKTKSRILEAVHESASDLHRLGFIDKRKMQKYDALCLEPVQD
                     YDAEKVKALRERLHLSQAVLASVLNTSTSTVRKWEVGDKRPSGPSQKLLDILERKGLE
                     AVL"
     gene            complement(11249..11336)
                     /locus_tag="PK242_01340"
     tRNA            complement(11249..11336)
                     /locus_tag="PK242_01340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11300..11302),aa:Ser,seq:tga)
     gene            complement(11452..11622)
                     /gene="rpmG"
                     /locus_tag="PK242_01345"
     CDS             complement(11452..11622)
                     /gene="rpmG"
                     /locus_tag="PK242_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOZ92700.1"
                     /translation="MATKGGREKIKLESTAGTGHFYTTSKNKKTMPEKMSIMKFDPKA
                     RKHVEYKEVKLK"
     gene            complement(11643..11876)
                     /gene="rpmB"
                     /locus_tag="PK242_01350"
     CDS             complement(11643..11876)
                     /gene="rpmB"
                     /locus_tag="PK242_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOZ92701.1"
                     /translation="MARVCEVTGKKPMVGNNVSHANNKTKRRFLPNLQYRRFWVESEN
                     RWVRLRVSGAALRLIDKIGIDAVLADMRARGQA"
     gene            complement(12078..13040)
                     /gene="trxB"
                     /locus_tag="PK242_01355"
     CDS             complement(12078..13040)
                     /gene="trxB"
                     /locus_tag="PK242_01355"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOZ92702.1"
                     /translation="MTAQHAKVLILGSGPAGYTAAVYAARANLHPLLVTGMAQGGQLM
                     TTTEVDNWPADVDGVQGPDLMQRFQAHAERFQTDIVFDHIHTADLSSRPFRLTGDSAS
                     YTCDSLIIATGASARYLGLPSEQAFMGRGVSACATCDGFFYRDQDVCVIGGGNTAVEE
                     AVYLSNIARKVTLVHRRDKFRAEPILVDKLMDKVSAGKIELRTFHVLDEVLGDDSGVT
                     GIRIRHVDSDATEDIRLQGCFIAIGHSPNSEIFKGQLDMDGGYIRTQGGLSGFATQTS
                     VPGVFAAGDVQDHVYRQAVTSAGTGCMAALDAQRFLEQQAERGA"
     gene            13181..13855
                     /locus_tag="PK242_01360"
     CDS             13181..13855
                     /locus_tag="PK242_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ92703.1"
                     /translation="MTMLSSLELIRRVPLFSMLTDAQAVSVADAVVKRRFKRGEVIVE
                     QGKKTNALFILLNGRARVVTADSRGREVILATLRPGDHIGEMSLIDNEPHSATVRAEV
                     QTDVLMLGRTEFARCLPENSSMAYAVMRGLVQRLRQADRKIESLALMDVYGRVARALL
                     EFASEVGDGALLIRERLSRQDIAKMVGASREMVSRVMKDLEERGFIQTQDDGAILVKD
                     RLTTLS"
     gene            13912..16251
                     /locus_tag="PK242_01365"
     CDS             13912..16251
                     /locus_tag="PK242_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOZ92704.1"
                     /translation="MAYPLNTFNGAPAAPTVRSGVGRFAQEVFLVLGLALIIFLVMAL
                     LSYSPQDAAWSTTGAGGPPHNWAGKLGGWLADLAYMLLGFSAWWCVAAVVRVWWGGVR
                     RWMHGGSPAAPAAGDSPWSRLRSARATFWVGLALLLAASTGLEWSRLYRWDHALPGSA
                     GGILGALGGPLAVHWLGFTGSALLGIAVLVLGMALVFRFSWGRLAEHIGAAVFALLES
                     RRERRALAQDLALGKQAARERQEVVHQEREVIEDQHPTPVLIEPPVVQVPKSERVVKE
                     KQKPLFTELPDSPLPQVDLLDGAPARQETVAPETLEMTSRLIEKKLKDFGVEVRVVAA
                     APGPVITRYEIEPATGVKGSQVVNLAKDLARSLSLVSIRVIETIPGKNYMALELPNAR
                     RQSIRLSEILGSQVYNDARSLLTVGLGKDIVGNPVVADLARMPHVMVAGTTGSGKSVG
                     INAMILSLLYKAEPKDVRLLMIDPKMLEMSVYEGIPHLLAPVVTDMKQAANGLNWCVA
                     EMERRYKLMSKAGVRNLAGFNQKIDDAKARGEFIYNPFSLTPEDPEPLERLPYIVVII
                     DELADLMMVVGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIAF
                     QVSSKIDSRTILDQMGAEALLGMGDMLYMASGTGLPVRVHGAFVSDDEVHRVVEYLKS
                     QGEPNYIEGVLEGGTAEGDAGAFGEGGDAGGEKDPMYDQAVEVVLKNRKASISLVQRH
                     LKIGYNRAARLVEDMENAGLVSAMTADGKRDILVPARPE"
     gene            complement(16303..16554)
                     /locus_tag="PK242_01370"
     CDS             complement(16303..16554)
                     /locus_tag="PK242_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HOZ92705.1"
                     /translation="MALMITDDCINCDVCEPECPNQAIAMGEEHYEIDPDKCTECVGH
                     FDQPQCVQVCPVDCIPVNPQRMEDPQQLLDKFRRLLGQA"
     gene            16641..17609
                     /locus_tag="PK242_01375"
     CDS             16641..17609
                     /locus_tag="PK242_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ92706.1"
                     /translation="MSYFLFKRILTLLATLAATSVIVFAVLEVLPGNAAQVLMGPDAD
                     PAAVAAKVVELGLDRPPLARYVDWMRGLLRGDMGNSYAYGSPVAELIAERLLLTVPLA
                     VLAMTLTALLALAVGVYAASQHNRLGDTGLMALAQVGIAIPNFWFAILLILLFSVKLQ
                     WLSAGGFDGWYFDDGVWAGLWQGLKALILPAVALALVQAAILARFTRSAVLEVLREDF
                     VRTARAKGLSRRATLWGHVLRNAMIPVITVMGLQSAELLAGTIVVESVFYLPGLGRLI
                     FQSIANRDLIVVRNCVMLLAAMVVVVNFIVDVLYAVIDPRVKASEV"
     gene            17773..18648
                     /locus_tag="PK242_01380"
     CDS             17773..18648
                     /locus_tag="PK242_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ92707.1"
                     /translation="MSGTLRLAAAVGAAPGFWARARRHPGFVIGALLTLALLLSAALS
                     YVWTPYSVYEVQMDDKLLGPDARHWLGTDAFGRDVVSLLLVGARASILVGVIAVGIGL
                     VFGAALGLLASARRGWVEELIMRVADFGFAFPAILSAIMLTAVYGPGIVNAIIAIGIY
                     GIPAFARITRAAANAVWSRDYVQAARASGKGAWAITWQHVLPNILAVLIVQATIRFAV
                     AILAEAALSYLGLGTQPPQPSWGRMLAEAQSLMFDAPLLAVWPGLAIALSVLGLNLLG
                     DGLRDVLDPRLQGSR"
     gene            18692..19024
                     /locus_tag="PK242_01385"
     CDS             18692..19024
                     /locus_tag="PK242_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92708.1"
                     /translation="MTLTVKSPPYLEQSLGQQSAAEGRSLSELWRDALTADLASARHA
                     HGDEVLAHKRAGRIARRFARMPLNPAGASRAEAAARRGLRRILGIDAACIVRRDSRGR
                     PLPNLLSS"
     gene            19029..20039
                     /locus_tag="PK242_01390"
     CDS             19029..20039
                     /locus_tag="PK242_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ92709.1"
                     /translation="MALLQAEHLRVSLRTHRGSATAVRDVSFALERGDTLGLIGESGC
                     GKSMTALALMGLQPDNATVQGSLRLDGQELLGLPDRDWRRLRGNRIAMIFQEPMTALN
                     PVHRVGAQIAEPLRLHRGLSAQAARQRAIELMARVGIPEPARRIDSFPHQFSGGQRQR
                     IMIAMALACEPDVLIADEPTTALDVTVQQQILALIRELVAERGMALVLISHDLGLIAQ
                     NVRRMLVMYGGAVVESGPAAELFRQPAHPYTRGLLAARPNLAQRLHAHRSDRLPTIAG
                     SVPDLFGLPPGCPFAGRCAYTEPACLTDPAPPVALTASHRFWCRRADTVLHAPESIAG
                     GA"
     gene            20039..20857
                     /locus_tag="PK242_01395"
     CDS             20039..20857
                     /locus_tag="PK242_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ92710.1"
                     /translation="MDKRDNHFLLETRELERRYTLPRESLFRTPGQVHALRGVSLQVT
                     AGRSLGIVGESGSGKSTLARLVMALERPTAGQVLLQGRDLHALSPDELRAARRDFQMV
                     FQDPYGSLDPRRRIWRTVAEPAAALEGADRAEQRERAAAMLTAVGLHADALDQYPHEF
                     SGGQRQRIAIARALITRPKLIVADEAVSALDVSVQAQVLNLMADLRDAHGVTFLFISH
                     DLAVVAHLCQDVAVMQGGRVVEHGPVRQVLREPRHPYTRQLLDAVPVSALLAAV"
     gene            21082..22569
                     /locus_tag="PK242_01400"
     CDS             21082..22569
                     /locus_tag="PK242_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ92711.1"
                     /translation="MKRRDFALSLPALAASTHLPALAQAGKSTMVLGMTLEPPGLDPT
                     AGAASAIAEVVHYNILETLTKINPDGSVSPLLAERWEVSPDLRTITFYLRKGVKFSNG
                     EPFNAQAVRFSFTRAAGDKSTNKDKRTFAAMESVSAVDEHTVVIINKEPDPDFLFYMG
                     QATAVIVEPKSADGNNARPVGTGPYELDNWAKGSSITLKAWPGFRNASAIRIKRATFR
                     FISDPAAQVAAVLAGDVDAFPRVAPRGASQFKANPRLQTLITGSRAKTVLAINNARKP
                     LNDVRVRRAISMAIDRKAVIAGAADGYGVPIGSHYVPGAPGYVDTTGINPYDPEKAKA
                     LLKEAGVTTPLELSLVLPPPSYARQGGEVIVAQLAKIGITAKVQSVEWAQWLSNTFGG
                     PKNYDLTIISHVEPFDLGNFAKPDYYWGYQNPKFNELYTRIKNTPTAAERNKLLGDLQ
                     KLLANDAVLAWLFQPQGVTVANKRVQGLWKESPIFVNDLSSLSWG"
     gene            22645..24069
                     /locus_tag="PK242_01405"
     CDS             22645..24069
                     /locus_tag="PK242_01405"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOZ92712.1"
                     /translation="MTEPLHDLSAHALLAGYRARHFSPVDVLDSVLHHVERWEPHIAA
                     SWLLRPDAVRAQARASEARWRRGEPCGALDGVPVTIKDNIATAGDPVPLGTRAVPLLP
                     ATADAPPAARLREAGAILLCKTTMPDYGMLSSGLSTFHRLSRNPWDLALTPGGSSAGA
                     GAGAAAGYGPLHLGTDIGGSIRLPAAWCGIVGLKPSLGRVPIDPPYMGRAAGPMTRSV
                     QDAALLMQVLSQPDARDCMSLPPQAIDWSLAWRQDKPLKGLRIGLLLQAGCGLPVEPA
                     IRAAVEQAARLLEGQGAIVTPWAPFMTPELLAGLDHFWRMRSLLDLDALSAPARASVL
                     PVIRDWAESARGMSGADLFRAYAATHATRVATSQAMQGLDYLISPVSPNAPAPAEWPS
                     PTNDPLRGLEHIGFTVPFNMGEQPAISINCGYMASPATPGAMLPIGLQIAGQRFDDLG
                     VLQLARAFEQLRGPQRPWPAPPAP"
     gene            24134..24988
                     /locus_tag="PK242_01410"
     CDS             24134..24988
                     /locus_tag="PK242_01410"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HOZ92713.1"
                     /translation="MSFPHNADLHCHSVVSDGTLTPEQLAARARANGVDLWSLTDHDE
                     VGGQQRALEAARAEGLRYLTGVEVSVTFADETVHIVGLGFDHTDPRLAEGLADTRDGR
                     GPRAVEMGEQLARVGIAGAYEGALRYVGNPRLISRTHFARFLVETGVCRDTAEVFRRY
                     LVEGKPGYVPHRWARLGDAVRWITQSGGVAVIAHPARYDISPTVEYALFSEFKQHGGQ
                     AVEVITGSHTAAEAARYAAMAREFGLAASRGSDFHSPDESHTDLGQLPPLPADLTPVW
                     TLLANRIH"
     gene            complement(25174..25815)
                     /locus_tag="PK242_01415"
     CDS             complement(25174..25815)
                     /locus_tag="PK242_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOZ92714.1"
                     /translation="MKARSLQDLKQLRTQLAEREARAAQAQAERAAAQQRARADQDLF
                     ARAIGATQPLPAHGRVVHAPPAVAPEPRQRQRDEQAVLRESISDEFDTSTLLHTDEHL
                     SFRRPGIGPDVAAKLRRGHWAVQGEIDLHGLRTDEAREALAQFLREAQRAGLRCVRVV
                     HGKGLGSPGRTPVLKAKVQVWLVQKAEVLAFVQAKPLEGGAGALLVLLAASIR"
     gene            complement(25812..26489)
                     /gene="radC"
                     /locus_tag="PK242_01420"
     CDS             complement(25812..26489)
                     /gene="radC"
                     /locus_tag="PK242_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOZ92715.1"
                     /translation="MSLKDLPADARPREKLLARGAAALSDAELLALLLRTGTAGRGVL
                     QMAQELLDRFGGIAGLLHASADDLGRFKGLGGSAKRAELIAVLELARRALAEQLKEKP
                     ALDSASAVKQYLQLHLGQRPHEVFAALFLDAQHRLVALEELFRGTLTQTSVYPREVVL
                     RALHHHAAAVVLAHNHPSGQVAPSRADEALTQTLKAALSLVDVRVLDHIIVAPGQALS
                     MAEQGLL"
     gene            26546..26989
                     /locus_tag="PK242_01425"
     CDS             26546..26989
                     /locus_tag="PK242_01425"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOZ92716.1"
                     /translation="MSTVEPGAFLTLHYRLSGPQGDVINTFGGPPATLSLGAGELSPA
                     IEQRLIGLAEGARATFELPAGEAFGERSPDMIQWLARQDLDELGDPQERYAVGDVIQL
                     PTPDGQGQFGAVVQAVRDDGALRLDFNHPLAGQPVTFEVQVIGIL"
     gene            26989..28035
                     /gene="ispH"
                     /locus_tag="PK242_01430"
                     /gene_synonym="lytB"
     CDS             26989..28035
                     /gene="ispH"
                     /locus_tag="PK242_01430"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOZ92717.1"
                     /translation="MPQSHGRSITITRKAAAADGEAAAAPPVDAHILLAEPRGFCAGV
                     DRAIEIVERALVKFGAPIYVRHEIVHNTYVVNDLKAKGAIFIEELAEVPPGATLVFSA
                     HGVPKAVEREAQARGFRVFDATCPLVSKVHVEVAKLHREGYEFIMIGHKGHPEVEGTM
                     GQLPGGIHLVEEVQDVARIRPTQTERLAVVTQTTLSVDDAAEITAAVKARFPMVREPK
                     QQDICYATQNRQDAVKLLSREVDVVIVVGSPTSSNSNRLREVALKLGTAAHMVDSADD
                     LKPEWVQGRARIGLTAGASAPDVLVRQVIERLRALGAVSVRQLAGIEETMKFPLPKGL
                     KLDSASEDGLAHLR"
     gene            28145..29455
                     /gene="serS"
                     /locus_tag="PK242_01435"
     CDS             28145..29455
                     /gene="serS"
                     /locus_tag="PK242_01435"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOZ92718.1"
                     /translation="MLDPLLLRKDLDGVVARLLTRKNPQPFLDVAAFQALEAERKAIQ
                     TRTEELQAQRNSASKQIGQRKAKGESADDLMAQVASIKDELDASAARLERIQDELQTL
                     LLAVPNLPHDSVPVGADEAANHEVRRWAPHGLAPRSFDFAVRDHVELGEPLGLDFETG
                     AKLAGSRFAFMRGPVARLHRALAQFMLDVQTGEHGYTECYTPYIVNAETLRGTGQLPK
                     FEADLFAAKKGGQEGEGEPLYLIPTSEVSLTNLVRGEILAEADLPLQLTAHTPCFRSE
                     AGSAGRDTRGMIRQHQFDKVEMVQIVHPERSYEALEQMVTHAEAILQKLELPYRVVLL
                     CTGDMGFGASKTYDLEVWVPAQNTYREISSVSNCEAFQARRMQARFKTAQGKNELVHT
                     LNGSGLAVGRALVAVLENHQNADGSIGIPRALQPYLGGIERLAP"
     gene            29502..29591
                     /locus_tag="PK242_01440"
     tRNA            29502..29591
                     /locus_tag="PK242_01440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29536..29538,aa:Ser,seq:cga)
     gene            29973..30104
                     /locus_tag="PK242_01445"
                     /pseudo
     CDS             29973..30104
                     /locus_tag="PK242_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
     gene            complement(30040..31452)
                     /locus_tag="PK242_01450"
     CDS             complement(30040..31452)
                     /locus_tag="PK242_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ92719.1"
                     /translation="MRASTLAQRLTRALVLRLALVWLLCVLAVAYYLNHEIEENFDAE
                     LAESAHRMFDVAMFQLQRGAPAGAAGQAPRADAVIADDPLFADAPLVYQLVDAQGRLL
                     LRSRQAPAQALAVPLEPGFHDTNQWRVHVAQHPEQALFFLLADPLSERNHEWRETLLV
                     LLVAAVLALAALAWALPGVVTRELGVLERLQRQIRRRGGGDLRPLTLTGLPAELRAVG
                     EDVNLLLQRLDQALDVERALAANAAHELRTPLATARLRLQTALEHGLHRADVQAAVDA
                     LRTLSQRTEKLLQLSRAESSAPLSREPVDLVRLAATVAQEFWQDERAADRLELITATD
                     GEHCTALGDFDALAIALRNLVENALHYAAPGPVEILVGPGPMLAVRDHGPGVSADRLK
                     SLQQRHVRQSADRAGYGLGLSIVATIAAKHDAQLTLTSPLPHESGGFEARMQWTVATP
                     PERASSRAAAPPSAPESPPR"
     gene            complement(31449..32120)
                     /locus_tag="PK242_01455"
     CDS             complement(31449..32120)
                     /locus_tag="PK242_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ92720.1"
                     /translation="MRVLLVEDDLALSDAVCGYLRAKAFVVDATASLSEAEAALHGAQ
                     YAAVLLDLHLPDGDGLALLPQLRALQQRPIVIVLTARDQVSDRIRGLDAGADDYLIKP
                     YDPGELLARLRAVERRRSAAETPVLVLGDLQIDLAREQVRRASVPIALTQKEWALLRV
                     LATRPGRIHTRENLQDALYGWGDETDSNTLEVFISRLRRKLGRTHIETVRGLGYRLHS
                     EPAAA"
     gene            32208..32906
                     /locus_tag="PK242_01460"
     CDS             32208..32906
                     /locus_tag="PK242_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOZ92721.1"
                     /translation="MFDVLWRRLALVLLPLVPLLLWDASGLDLPLARLFGSAQGFAWR
                     SHPSYVLLLHEVPRALSMAALLALAWGAWRPWGFLRTMGAADRRQLAFSVALAMLSIT
                     ALKRLATTSCPWDLAEFGGVAHYVSHWIWGVRDGGPGHCFPAGHASAGFGYVAGWFVL
                     RRAVPRLATGWLLASLVLGCALGLSQQVRGAHYMSHTLWTAWFCWALGLLVDEAVQRW
                     RSRTRARDTNLNET"
     gene            33055..34707
                     /locus_tag="PK242_01465"
     CDS             33055..34707
                     /locus_tag="PK242_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HOZ92722.1"
                     /translation="MATPLTAQQLVLRLSLYLVLAANWPLWLQIMRIGGAPSQYLRSM
                     AALAVLIVCGMVAIFAFFAWARGMRLLWWLVVLVAALAQYYMLTYQVVMDPGMAANVL
                     QTDAREAADLLSLRMLLTVLAVLVLPSWWLLRVRIVAMGPLAQLWRNLVMLVLALAVA
                     AGTVAATARDLAPLMRSHPQLRYLMNPLASLYSTPVAALRPVFARSRTLVPMTQGAAL
                     GTSYAAGARPMLFVLVVGETARADHFGINGYGRDTTPELARRDVLSWREVRSCGTSTL
                     ASVPCMFSPLGKDAFESRDNEYENLLDVAQTAGMAVLWLDNQSGCKGVCDRVAHASAS
                     EALPRDAQAALCPGGECGDDALLHDLDARLAALPAAQRERGVLLVLHQMGSHGPAYHK
                     RSHGAAKRFLPECRTEVLADCAQQELVNAYDNSIAATDLFLGKTIDWLQARSARYDTG
                     LLYLSDHGESLGEYGLFLHGMPYNMAPDVQKHVPMVAWLDDALLRRERLSDACLRAGA
                     NAPLTHDNLYHTMLGLLDVQSPTYTRALDAFADCRRVALAAR"
     gene            34975..35940
                     /locus_tag="PK242_01470"
     CDS             34975..35940
                     /locus_tag="PK242_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ92723.1"
                     /translation="MNLDHGIHVIDTGFVRPHLDAAYLIVERGRGAFIDCGVNHSVPH
                     LLAALAQAGLTPGDVDWLILTHVHLDHAGGAGELLRQLPQARLVVHPRGARHMIDPSR
                     LWAGAAAVYGEAEMQRSYGRLLPVPAERVVAAPDGHVVELAGRPLVCLDTPGHARHHN
                     AIHDLASGCVFSGDTFGVSYREFDTEQGAFILPTTSPVELDPPALHRSIDRLLALQPP
                     AICLTHYGPVAPPARLAQDLHAQLDAMVELAHAAAPQPSDERHRSLRAGLLELYARRA
                     AQHGWRGGRERLAELLAVDIELNAQGLGVWLDQSTRMTKDPKNDS"
     gene            complement(36200..36802)
                     /locus_tag="PK242_01475"
     CDS             complement(36200..36802)
                     /locus_tag="PK242_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOZ92724.1"
                     /translation="MNPADALSAADWLTLWLHFLSLSLLAVGGAITTAPDMHRYLVGS
                     QHWLSDAQFNASIAIAQAAPGPNVLFVALVGWHVGLNAGGGPAGGWHAQGLALLGAAV
                     AMLGILLPSGVLTYSATRWAHQRRELRAVRAFKAGLAPIVIALLMATGWLLSTAHGQP
                     ARDWPLWLLTAATTVLVWRTRLHLLWLIGAGALLGMLGWI"
     gene            complement(36799..37386)
                     /locus_tag="PK242_01480"
     CDS             complement(36799..37386)
                     /locus_tag="PK242_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOZ92725.1"
                     /translation="MSATPPAAAGDGTLGAPRSRADLFWSFSWLALQGFGGVLAVVQR
                     ELVERKRWLTREQFVEDWAVAQIMPGPNVVNLSLMIGDRHFGLSGALAALAGMLTLPT
                     LAVVLLAALYSNVADVPLVQGALRGMGAVAAGLILGTGIKLIGALRANPLGMPACIGL
                     ALATFVAIGLLRLPLGPVLLVLGGAACVWAWRRLP"
     gene            complement(37484..37837)
                     /gene="arsC"
                     /locus_tag="PK242_01485"
     CDS             complement(37484..37837)
                     /gene="arsC"
                     /locus_tag="PK242_01485"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722955.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HOZ92726.1"
                     /translation="MATVTIYHNPHCSTSRKALELLRSRGIEPTIVQYLKTPPSAAEL
                     QAIARASGAPLRALVRTKQPEYLAQGLDDATLTDAQLAAALHATPVLIERPIVVTARG
                     ARLGRPVENILDLLD"
     gene            complement(37917..>38809)
                     /locus_tag="PK242_01490"
     CDS             complement(37917..>38809)
                     /locus_tag="PK242_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOZ92727.1"
                     /translation="LLVDLQDLGCRIYTFITTLRYVLEAAAAHGKAVWVLDRPNPVGR
                     PVEGLTLRAGWESFVGAGPLPMRHGLTLGELGHWFIAQLGLQVEYRVIAMEGWRPDAA
                     PGHGWPLERTWVNPSPNAPNLWMARAYAGTVMLEGTTLSEGRGTTRPLELFGAPDIDT
                     ARLLADMRALAPDWLRGCRLRECGFEPTFHKHAGQMCAGVQIHTEDATHYDHTAFQPW
                     RLQALAFKALRRQAPDYPLWRDFPYEYETRLAIDVINGGPLLRQWVDDAAATPAELDA
                     LTRADEQAWRAARTPFMIYE"
BASE COUNT         6546 a        12677 c        12978 g         6608 t
ORIGIN      
        1 cggttgacgc gtttcaaggc aaagagtttg acctcgtctt cctgtccgta gtgcgagcta
       61 acgacgtggt gattcccgat cagaaggaag gggaagagcg cgaacgattg ctaaacagca
      121 agtatggaca cttgcggttg gcaaatcgca tgaacgtggc gatgagtcgc cagaggaagc
      181 tcctcgtcgc cgtcggtgcc aaacacatgg cagaaggacc cgagtccgag gaaggggtcc
      241 cggctctggc tgcattcctc aagctttgca gagaggagat ggcgcatggc cgctgaccgc
      301 tatcttcgct tcgatgtccg tcgccccccg ggtgggcggc cgcttctgtg gcctgtccgc
      361 gtttggaagg tcctttatcc aacaaatcgt gccctcaagt tgaacctgtt ccagcaggcg
      421 atccttggac tggcaagggc cagatgccag gacagttcgg aaatggctga gttgctgggg
      481 ctggaccgag agctggtggc attcatcatt gcaactcaac tgatcccgaa cggctggatg
      541 accacctcgg gatcagtcac ctcgcaaggt gagcgtgtcc tcgaagaggc tcaggatgcc
      601 agcgaggaag tccgcatggg gtacgcatat caggacgcga tttcggggaa ctggctgccg
      661 cgattcactg aagaacttcc agagatcgaa gcgaagcgag tcgatgaacg tggttaccca
      721 gtgttcctgc gagacctcga ttccggaaag gaggatcgcc ccttccgcct caatcacttc
      781 cgagaggacg cactggacac agcggcactc tttgatgcgt ttcagcgcta tcgaacggat
      841 cacgaccacg ccaagcagcg tgatgaggag ctttcggcac gtgtccgcat tgagagcctg
      901 agctttgtcg aagattcggc gcagccgatg tggttatgga catggatttt tcccgacgag
      961 gctgggccac aaccctggtt ggttgccgat ccattcgggt tgcagcaggc tgcgtcgtgg
     1021 ttgcgcaaac ccctgcagga cgtcttgccg cgaaacgatg gacttgcaag atacatcgct
     1081 gacgcggtag gcgagacgag atcgaacgat ttgtcggcag aggagtggct ccgatccctg
     1141 gagaatcaga tcgacttgac actgcttgca gactacaact ggtcgcggaa ggtgccgatt
     1201 gttgagggat acctcgccag cgttctgaga cgaagatccg tgctggctag tcaggagagg
     1261 tcttggcagg aggatgtgac tagccttctc attgagactc acaacctggc agaaagcgtg
     1321 ctgcagtggc tgttgaagca attttccgtt gatgtccgca gacttccaga taaaagccaa
     1381 gacaacaagt ggacgaagca aatcgccgta caaacattgc aggcgctccc catagcaaaa
     1441 ctcgacgacg aattagttag acgccttgcg tcacagaatt tgcgagaaat tagaaatgcg
     1501 ctgatccgag gcaatagctc actgaaagct ctgatgtttg ctgcactgct ttctaccatc
     1561 gagcactccg accacccctt cctcaagcta tccgctgatg agcttcgact gactcgtctg
     1621 cttgatatgg cagatgctcg caacaaaaaa gccgggcatt caggtagcga gagaatcgat
     1681 agagaagaag ctatggaaca cgctgacttt gtcatcgaat gggttcaaat ttttaagggc
     1741 tggtattgaa atggcaaaac acaataaggg gaacaaagcc ccagtcgcgc aaaacgcaaa
     1801 tactgcacaa agtgcggtcg catcggcatg gcagaaagca actgctggaa ttggctccct
     1861 tgcatcgctg gtgcaaggcg atgcttccgc tgtggtactc cctgacttgg agaaagatca
     1921 ggtcagcgag ttggataagg tagagcccga gaaaaaaatc gagctcgaga ggttgctggc
     1981 ggatatctct gatgccgcaa agagagttcg tgatctacat gaagacgcac agaagcgctc
     2041 cgatgacctg gctcgcaagg aggaggcatt ctcccgccga agcgatgaga acaagaagcg
     2101 aaccgaagag atcaacaaac tcgacgaaga gttgaagcaa agacaagttg agataaaaaa
     2161 gtccgaatca agtttggccg ataaggagcg tgaacttgag aagcgagagc tggatgcgcg
     2221 ttccggtttt gccgcccaaa acgtggccgc actcagtgat ctcaagaaag aaatcgtgga
     2281 gcttgagtct aggaagactg agattcaact cggcatcctt caagcggagc agaaagcacg
     2341 tgacgatgag tccgcgcgcg catccgaagt gatggaacgt gaagcggaga ttgcaaacaa
     2401 ggagagagct ctacagcagc agcaacggcg actagatgca gagtggaaag agcttgagcg
     2461 tgaacgggta cagatccgcg aagattcact tcgcgaggcc agtcaggaaa ttgccagatt
     2521 gaccgatgct cggctacgcg cagaggcacg ccttgaaaag gtttaccgtg attggacatt
     2581 gactgaacag aaactagagc aataccggga gttctcagag gcattggcag ggaggtctgc
     2641 ccgcgagata cttgacgagt tggctgctca gaaacgtcga gtcactcaat tggaaaacca
     2701 aattgcaaac agtcaggatg atcagttgca atttgagaac gaggcgctgc gaaaacttcg
     2761 tgatgagcaa aaggctcaac tcgacgaggt tcattttgat ttggctgatg ctaaagctca
     2821 gcttcatcgc ctccgcctcg gcgtgagtga caaagagaac ctcgaaagag aaaagcgtgc
     2881 cctggaaaag aacaatcaga tcttgagtgc aaggctcaat gacttgggca aaacggtaga
     2941 tgacctaacc cagtcacagc aatctgagaa gccgttccct caaatggcat ggatggattc
     3001 cgcatccact gccgattgga ttaaggatcg caaggccaag gagttgagcc tgccggagca
     3061 agacgttcct gatctgaaaa aattctcggt tgaattgcag caccggatcg ctcaagcgga
     3121 ggaaggcgcg atactgcatt tcagcctcga agatattcag ttacttattg ccgggttggc
     3181 catgagccag cttcacatct ttcagggaat cagcggcacg ggtaaaacga gcctggcgaa
     3241 ggcgttcgca aaggctgttg gagggcactg cactgacatt gccgtccaag ctggctggcg
     3301 agaccgtgat gatctactag ggcactacaa cgcattcgag aagcgcttct atgagcgtga
     3361 ctgcctgcaa ggtctctacc gtgcgcaaac agccgcctac aaggaccggt gcaacatcat
     3421 cttgctggac gagatgaacc tgtcgcgccc ggagcagtat tttgctgaat tcctctcggc
     3481 gctcgaaaag aatgacccaa gagatcgact catcagtttg tcggagagcc agttgccaaa
     3541 cgcaccagcg ctgctggtcg agggtcgaag aattcgggtt ccccacaacg tttggtttgt
     3601 aggcactgct aaccacgatg aaacgacgaa cgagttcgct gacaaaacct acgaccgtgc
     3661 tcatgtcatg acgcttcccc gccacgaagc tggattcaag gtggagccga agccgaaggc
     3721 cagctttagc tatggatcat tgatggagcg tttcgatgct gctgtgacac aaaacgctga
     3781 tgaggtttca gagttgttgg cggagttgac cacgggcccg ctcacaagca tcttgcagga
     3841 tcgctttgac ctgggttggg gcaatcgact cgaacgtcag gccatgcgat ttgtccctgt
     3901 ttacatggct gccggcggtc gcaaagagga tgctctggac catttgcttg ccagtagagt
     3961 attcagacgc gggaaggtca ctggcagata cgatgcgacc atcgatgatc tagccgccat
     4021 cgaacaagcg ctgaccacag tatggaaggg gtggaaatcg gagcctcgcc gttccattaa
     4081 cttgttggct gaagaccgtc gtcgtaagga acgtggggca tgatgtttct ggaccgcctt
     4141 acgggtggca aactcagccg cctacccgac gcacttctgc cggggcggta ttgcttgctt
     4201 gagccactcc tggtcaatgg acacagccgc ttgcagcaag gtgatctgct ctcagatgac
     4261 ggctcgggcg taatccggac tcaagacaca gaatcgcaag tgctcgccat cgatcagtct
     4321 gacgacgatc aggcggactg cgatctgtcg gcagaagcga ttatttccat cgccgggaaa
     4381 atcgctcacg cacatgatgc aaatgtctca cccatgctgc ccgctgagat ggcagcccaa
     4441 tgcgcgctcg aagaactcga gcgcgacttg gcggtggtcc tcagagacgg tcatctgcat
     4501 tcaatatctg accgcccccg cagagacctt cgatatgacg acttggttgc gccagtggcg
     4561 cgagctcgac gcctcgcgac ttcggcatta agtcacctgg catcacactc tgattgctgg
     4621 cagcagcgta cgctcagcgg tgttcaaccg cgcaaggttc ttgcacgttt cagcgaggac
     4681 gactatgcca tctatgagaa caggctctac aagcgcttac tggatcggct agatcgacac
     4741 cttgccagga gattggctcg catccgggga gtcaattcac gactggagcg agctttggag
     4801 ttccaagact ctgagcagac gcattttcga cttcgccagg atatttgtcg cctttggggc
     4861 gagtcatatc ttgatgacaa gactgggacg cagttggagg caggaaagcg tgcactcagc
     4921 gacttggagt ctcagttgcg tgtaatcaga ggactcaagc aaagggggct ttattctctg
     4981 gtgcctcctg catctgtggt tcctgcccaa gttcaccgga caaacattct caaccacgac
     5041 ccgcactatc gtcatctgcc cccgctttgg gagaagttga aagatgatcg agaagaccgg
     5101 ctcttacccc cggaagagcg cttggcgcga caactgcaat tgcagctggc ttacgtcagt
     5161 tacgttggtc ttgtgatcag gcgggcgctt gagcggtatg gtctgcgcag agtggatgga
     5221 aatttcgtct tcagttgggc gggacggagt ttcgtactcc gacgtgatgt acatgattgg
     5281 gtcgtaactc agtctcaggg atctaccctg aggatcgtgc cgattgcctg gttcggcgcg
     5341 tcaataaata tcagtgagag tctggcgcct ggtcgcatcg tgtgttggcc aggcgggcca
     5401 accagtgtcg catcgccgca gagtcttcct gtctctccgc tggaccttta tgtcgtggag
     5461 aaggtgggga agttgatcga tgagtggatg ttgcggcaat tgctccaagg tcatggccga
     5521 aaattggggc cgttgcccac accggccaaa aggctaaccg aggcttggcc agaacagttt
     5581 gaatctattt cgtctaccca tgtcaggctc ctcgcgccag tcgatgctca gaaagcagcc
     5641 gaattgaagg cttcactcaa aggctcagct aattcgcaag ttgaggatgc cgtctgggac
     5701 gccatcgaac agatcgcaac tcttgctgag ctttgtggcc atcaggcgcg attcgtgcga
     5761 gggcagcagc aggacttcta ctgccaatgc ggaacgtgcc aggcaacttg gtcgctcaag
     5821 acaacaggga aaaggcgaca cttttctatg cggccgaagg gggcatctgc cataccagaa
     5881 gatgacggct ttctttggtc tgggagggat tggctggagt tcgatttgga ttcagaacag
     5941 tgacggtggc caaatcgtcg aatccacgcc ctcaaggcca aggtgttact atctggctct
     6001 gaaagcggac gcgggttcgg ttccgtgttc cgccaccaat cacaagggtc tggatagtct
     6061 ccaggccctt tttttgcctg aaatccccac gccacgcggg gttcctgcca cttgtgctgc
     6121 gggtggccac cagccgaaac gcccaaaatt ggccactttc tcggccgggt ccgtctcttt
     6181 tctctgtttg gcctgcgggt aggcgcagat ggcggtcttg caaaatcaac aatttacgcg
     6241 cgccggttca tcgtcaacca agaccagcgg tcaagcatca aaccccgcca tctggtggtc
     6301 gcactgattg catgggaact tatacgttac tatcatgact catgagctac cagatcaaag
     6361 aactgctact ggccgatggc gacagtccct ttgcgcagtg gttcgggtcg ctggaagccg
     6421 tcgccgccgc caaggtacgc gtggcggtga gccggatgga acaaggcaac ctgtccaacg
     6481 tggaatggtt ccgtggtatc ggcgagtaca agatcgattg ggggccgggg ctgcgcattt
     6541 atctggccaa ggacggtttg aagatcatca tcctgatcgg cggcggcacc aagaagcgcc
     6601 agcagcagga catcgaccag gcggtggcgt tgtgggacga ctacaagcgc cgcaaggcat
     6661 caacccagaa aggagggaag tgaaatggca ctgacccgcg atttcaaaga aaccgtggcc
     6721 gcgcgcgtgc agaacgatcc ggccttcgct caagccctgt tggacgaggc catcactctg
     6781 ttcgtcaacg gcgagccgga atcggccaag ctgatcctgc gcgacctcgt caacgccacc
     6841 gtcggcttcg aggcgctggc cgaagaaatt cacaagcccg ccaagagcct gcaccggatg
     6901 ctctcccagt cgggcaaccc gaccatgagc aacatctcgg cggtgtttgc agccatcaag
     6961 cgtgcgctca aggtcgaggt gcgcacgcag gtggtgatgg cctgacttca ggcatcaaac
     7021 cccgccacca ccgcccgctg cgccatccag tcggtcggca gcggattgcg ctggaaccac
     7081 agcaggctca tgcaccgggg ctgctggcca gccaggatgg tctggatgat ggcaggctcc
     7141 agcaaggtca ggcgcagcag ttcattgacc gtcgagtggt gcaggccttc gcgctgggca
     7201 atctcgctgc cactgccgac cacgccgtcg tcgagcaact gctgccagta gaaggcccgc
     7261 gtcaaggcca ccagcaaggg ctggtcgatc tggctggcga ccttgcccgg cgactcgacc
     7321 gccccatccg gccgcaccac caccttgctg gcgccgcgcc tcctgatctt cagcgggatg
     7381 aaggtggaga gtttcacctg cccgccatcg cgccgttcac gggcgatggg gccgcccaaa
     7441 gcctgtactt tcgatttcat gcctccacct cctgtcgctc ccattcctgc aattccgcgc
     7501 cgatggtgcc gggcaacaat tccccggcca actcctgcca gccctgatcg cgccagatga
     7561 tttccaaccc gccgtcggca atcaccacgc gctcgatcag gagttttgcc aaacggcact
     7621 gttccgcagg gaatagctcc cgccacaggc ccgcgagatt gcgcatcggc agcacgactt
     7681 gcggctcggt gagatcaggc cggatgtgct gcatccgatc ccacaccgcc tgcacgatgt
     7741 gtggggccga cagcgccgct acgatctgct gcgtcaccag atcctcaatc ggcgcggcag
     7801 gcaacgagcc gtgctgactg gcccacgcgc ccaagcgccg gtgtttgacg gggctgtaat
     7861 agcggtaggt cttgcccttc tttttcgtgt agctgggcac cagccgttcg ccgtcggacg
     7921 tgaacagcag accgcgcagt aacaccggtt cccgctggcg gtcattggtc tcgcgccgcc
     7981 gttcgacgcc atcggtggcg atcagcgcgt gggcggcatc ccattgcgcc tgcgtgatga
     8041 tggcctgatg ctggccgggg aaactctgcc ccttgtgcac gatcttgccc aggtacatcc
     8101 ggttgtgcag catcttgtag atggtctgct tggtgaaggg cttgccgccc ttggtgagca
     8161 cgccttcggc gccataggtc ttggccatca gcgtggccga gcgcatcgtc acgaagtcat
     8221 cgaagatgcg ccgcaccaga gcggcttcga cttcattgat catcagcagg cggtcgcgca
     8281 cgtcgtaccc gagtggcaac ggtcgaccca tccacatccc cttggccttt gcaagggcga
     8341 tcttgtcgcg gatgcgctcg ccggtcacct cacgctcgaa ctgggcgaag gacagcagca
     8401 cgttgagcat cagccggccc atcgaggtgg ccgagttgat ctgctgggtg acggcgctga
     8461 agctgacgcg gtggcgatca aacacatcga ccatccgcgc aaagtccgcc agcgagcgtg
     8521 acaggcggtc gattttgtag acgaccacga tgtcgatctt gcccaactgg atgtcggcca
     8581 gcaggcgctt caaggcaggc cgttccatat tgccgccgga gaagccaccg tcgtcgtaat
     8641 catcggccac ggcaatccag ccctcgtggc tctggctctt gatgaaggcc tgcccggcct
     8701 ctttctgggc atcgatggaa ttgaaggact ggtcgaggcg ttcatcgctg gaaacacggc
     8761 agtacaccca tcgccgtcgg cttcagcggc acgctgcgtc cagaccagga agctgccgtg
     8821 gcggcgatgc tgggtcacga tgcgggtgta ctgtgcgcgc caacggcctt tggcaaaacg
     8881 gtgacggccg cagcgatcat cgccccgcgc ggcgtcaaca ccttggttct ggtgcaccgc
     8941 acggaactgc tcaagcagtg gcaggtgcgc cttgccgcat tcctggacgc aggcaaggat
     9001 gtgatcggcg tcatcggcgg cggcaagagc aagcctaccg gccagatcga catcgcggtg
     9061 atgcaatcgc tttcgcgtca aggcgaggtc gctcccctgg tggaaagcta cgggcaggtg
     9121 atcgtggacg aatgccacca tgtcggtgca gcgtccttcg atgccatcct gcggcgtgca
     9181 aaggcgaaac acgtggtcgg cctgacggca acgccgattc gccgcgatgg gcaacagccc
     9241 atcatcttca tgcagtgcgg accgatccgg cacaaggcgg cgcagccgct tgatgcgccg
     9301 cacgatctgg cggtgacgcc gcagttcatc gagtcgccga ttgctctgcc agcggaggcg
     9361 ggcatccagg atgtgttcag gcatctggca caggacgcgg cccgcacggc agccatcgcc
     9421 agcgcgatca ccgacgcctt tcggcaaggc cgcaaggtgc tggcgctgac cgaacgcacc
     9481 gagcacatcg acgccattcg caccgcgttg gcgggtcggg tgccgcagcc gttcgtgttg
     9541 acggccggat gtcaaaaaag cagcgcaccg cgttgatcgc cgcgctcgat gccttgcagc
     9601 cggacgcgcc gcgcgttctg ctggctaccg gtaagctggt tgacgaaggc tttgaccatc
     9661 caccactgga caccttggtg ctggcgatgc ccgtctcgtg gaaaggcacg ctgcagcaat
     9721 acgccgctcg cctgcaccgg gagcacgcca gcaagaccga cgtgcgcatc atcgacttcg
     9781 tcgacacggg gcatccggcc ttgctgcgga tgtgggacaa gcgccagcgt ggctatcggg
     9841 cgatggggta tcgcgtcgat tcgggtgcgc cgtctgtcgg ccgtttcgaa tggtgatgtc
     9901 ggctgggcag ggtgccggaa caggcgggta gacaatctat gctactcggt ggcacaataa
     9961 agttgatgac gcgagtattc aaaactcgcc acttccagcg atggatgcgc aagacagagc
    10021 tcaccgatgc cgtcctgtgc aaggccgttc aggagatggc ggctggcctg atcgacgctg
    10081 acctcggcgg cagcgtggtg aagaaacgtg tcgggttggc ggggcgcggc aagcgtggtg
    10141 gcgtgcgcac gctggtcgcc accaacaagg gcaaccgttg gtttttcgtg ttcggcttcg
    10201 agaagaacga gcgggcgaac gtcagcgacg aggagctcga aggcttgcag accatcgccg
    10261 ccgacctgct ggctcgaaca ggcccgcaac tggatgaagc cgttgccgac ggcgctttgc
    10321 aggagatttg ccaatgacca ccaagaccaa gagccgcatc ctcgaagccg ttcacgaatc
    10381 ggccagcgat ctgcatcgcc tgggattcat cgacaagcgc aagatgcaga aatacgacgc
    10441 gctgtgcctg gagccggtgc aggactacga cgccgaaaag gttaaggcgc tgcgcgagcg
    10501 attgcacctg agccaagccg tgctggccag tgtgttgaac accagcacgt ccactgttcg
    10561 caagtgggag gtgggcgaca aacgccccag tgggccgtcg cagaagctgc tggacatcct
    10621 cgagcgcaag gggttggaag cggttctttg atccgcccat tgccgctcac gcccgctgca
    10681 tagctattca agcgacagag acggagttga actcgtcaac catccgccac cataatgtga
    10741 aatcccaacc cttatccggt tgggattttt tttgcccgtt cccccagtgt tggcgcggtt
    10801 tcgggccttg gcctcttgag cacgcccctc ccgaagtcgc cattccgggc acgcttttcc
    10861 ccgtgtttct ctctctgttc tctgttgccc tcttgagcat tcaaggcccg aagtcgtgcg
    10921 ttggcgcggg tttgcgggcc atcggtttgc atcttcgtct gctcgggagc gcgcgaccgg
    10981 gacagcgaag ggcgtaaaaa aaccgccttg cggcggcgtg ttggtgcggc acggacacct
    11041 tgccggcagg ggccgcgtga gcgggtgcgc cggacgcgct ttagtcggtc gagtcgcgcg
    11101 ttccaaagca accaccgaca gccggcgggg ccttggttgg gcccgtggcc ggcctcttcc
    11161 ccgtcacgtc aagcgccgtt cacggcatgg gttagaacgt gggtagaaaa ctgaaatcgg
    11221 cctgaaaccc gcatgaacac taggccactg gcggaggcgg tgagattcga actcacgaac
    11281 ggttgcccgt tgccggtttt caagaccggt gcaatcgacc actctgccac acctccgcgt
    11341 cgatgccgac gggcgattct agcggctgct cgcttgccgg acagccaaaa agaaacccgc
    11401 cggtcaggcg ggttggttca ggtcagcggg ctccatggcc ctctggctgc gtcacttcag
    11461 tttgacttct ttgtattcaa catgcttgcg agccttgggg tcgaacttca tgatcgacat
    11521 cttctcgggc atggtcttct tgttcttgct ggtggtgtag aagtgaccgg taccggccgt
    11581 ggattccagt ttgatctttt cgcgtccgcc tttggtggcc atgatctgta tcctttgcta
    11641 gcttaggctt ggccgcgcgc ccgcatgtcg gcgagcacgg cgtcaatgcc gatcttgtcg
    11701 atcaggcgca gagcagcgcc agacacgcgc aggcgcaccc agcggttctc gctctcgacc
    11761 cagaagcggc ggtactgcag gttcggcagg aaccggcgct tggttttgtt gttggcgtgg
    11821 gaaacgttgt tcccgaccat gggcttcttg cccgtgactt cgcacacgcg tgccatgagt
    11881 ggttgactcc agtccatccg gccttggctg acgcccactg cctcacatga agcagccaac
    11941 tcgccttggc ctcacctcgc cagatatggg tggcggtagc ccatcagaat gccggcgcgt
    12001 cagcacaatg ctgactgcgt cgggcaaagc aaagccaggc attatagctc gattggcagc
    12061 ggctcaggcg ccgcggctca ggcgccgcgc tcggcctgct gctccagaaa gcgctgggca
    12121 tccagcgcag ccatgcagcc ggtgccggcg ctggtgacgg cctggcggta gacatggtcc
    12181 tgaacatcgc cggcggcgaa cacgcccggc acgctggtct gcgtggcaaa accactcagt
    12241 ccgccctggg tgcggatgta gccgccgtcc atgtcgagtt gccccttgaa aatttcagag
    12301 ttgggcgaat gtccgatggc gatgaagcag ccctgcagac ggatgtcctc ggtggcgtcg
    12361 ctgtcgacat gccggatgcg gatgccggtc acgccactgt cgtcacccaa cacttcatcc
    12421 agcacatgaa aggttcgcag ctcgatcttg ccggcgctga ccttgtccat cagcttgtcc
    12481 accagaatcg gttcggcacg gaacttgtcc cggcgatgca ccagcgtgac tttgcgtgcg
    12541 atgttggaca aatacaccgc ctcttccacg gcggtgttgc cgccgccgat gacgcagacg
    12601 tcctgatcgc gatagaagaa cccgtcgcag gtggcgcagg ccgacacgcc gcgccccatg
    12661 aaggcctgtt ccgacggcag gcccagatac cgcgccgaag cgccggtggc gatgatcagg
    12721 ctgtcacagg tgtagctggc gctgtcgccg gtgagccgaa acggtcgtga cgacagatcg
    12781 gcggtgtgaa tgtggtcaaa cacgatgtcg gtctgaaagc gctcggcatg ggcctgaaag
    12841 cgctgcatca ggtcggggcc ctgcacgcca tcgacatcgg cgggccagtt gtccacttcg
    12901 gtggtggtca tcaactggcc gccctgggcc atgccggtga ccagcagggg gtgcagattg
    12961 gcgcgtgcgg cgtacacggc cgccgtgtat ccggccgggc cggagccgag gatgagaact
    13021 ttcgcgtgtt gggcggtcat cggtttgcct tgggaattcg cggaggctgc gctacgatat
    13081 gtggaaatgt gcagtgttct gcatgtgact acgaggtggg tgcagcgcag ccgggattct
    13141 agggattccg ggccggtgcc agacttggag atgaattgaa atgaccatgc tgtccagtct
    13201 cgaattgatt cgccgggtgc cgctgttctc catgctgacc gacgcacagg cggtgtccgt
    13261 ggcggacgcg gtggtcaagc gccgcttcaa gcgcggcgag gtcatcgtcg agcagggcaa
    13321 gaagaccaat gcattgttca tcctgctgaa cggtcgcgca cgcgtggtga ccgccgacag
    13381 ccgtggtcgc gaagtgatcc tggcgacgct gcggccaggc gaccacatcg gtgaaatgag
    13441 cttgatcgac aacgagccgc actcggcgac cgtgcgcgct gaagtgcaga ccgatgtgct
    13501 gatgctgggc cggaccgaat ttgcccgctg cctgccggaa aactcctcaa tggcctacgc
    13561 cgtgatgcgt ggtctggtgc aacggctgcg ccaggccgac cgcaagatcg agtcgctggc
    13621 gctgatggac gtttatggtc gcgtggcgcg cgccttgctg gagtttgcct ccgaggtcgg
    13681 cgacggcgcg ctgctgattc gcgagcggct gtcgcgccag gacattgcca agatggtcgg
    13741 tgcctcgcgc gaaatggtca gccgcgtgat gaaggatctg gaggagcgtg gcttcatcca
    13801 gacgcaggac gatggcgcga ttctggtcaa ggaccgcttg accaccctga gctgatccgg
    13861 cggcgcccgg gcgaacctgg ggtcgcggtt ccccaggcga tgctgttttc gatggcttat
    13921 ccactcaata ccttcaatgg cgccccggcg gcgccgacgg tgcgttccgg tgtcgggcgc
    13981 tttgcccagg aagtctttct ggtgctgggg ctggcactga tcatttttct ggtcatggcg
    14041 ctgctcagtt attcgcccca ggatgccgcg tggtccacca ccggcgcagg cgggccgccg
    14101 cacaactggg ccggcaagct gggtggctgg ctggcggatc tggcctacat gctgctgggc
    14161 ttttcggcgt ggtggtgcgt cgccgccgtg gtccgggtct ggtggggcgg ggtgcggcgc
    14221 tggatgcacg gtggctcgcc ggccgcaccg gcggctgggg attcgccttg gtcgcgcctg
    14281 cggtcggcgc gcgccacctt ctgggtcggt ctggccttgc tgctggctgc cagcacaggc
    14341 ctggaatggt cgcgcctgta ccgctgggac cacgcgctgc cgggatcggc gggcggcatc
    14401 ctgggggcgc tgggtgggcc gctggcggtg cactggctgg ggttcacggg gtcggcgttg
    14461 ctgggcatcg ccgtgctggt gctgggcatg gcgctggtgt tccggttttc ctgggggcgc
    14521 ctggctgagc acatcggcgc cgcggtattt gccctgctcg agtcacgccg ggagcgccgc
    14581 gcgctggctc aggatttggc gctgggcaag caggccgcgc gcgagcgcca ggaggtggtg
    14641 catcaggagc gcgaggtgat cgaggaccag cacccgacgc cggtgctgat cgagccgccg
    14701 gtggtgcagg tgcccaagag cgagcgcgtg gtcaaggaga agcagaagcc cctgttcacc
    14761 gagttgcccg acagcccctt gccgcaggtc gacctgctcg acggcgcgcc ggcacgccag
    14821 gaaaccgtgg cgcccgagac gctggagatg accagccgtc tgatcgagaa aaagctcaag
    14881 gacttcggcg tcgaggtgcg cgtggtggcg gccgcgcccg gcccggtgat cacccgctac
    14941 gagatcgagc cggccaccgg cgtcaagggc tcgcaggtgg tgaacctggc caaggatctg
    15001 gcgcgttcgc tgtcgctggt gtcgatccgc gtgatcgaga ccatcccggg caagaactac
    15061 atggcgctgg agctgcccaa tgcccggcgc cagtcgatcc ggctgtccga gattctgggc
    15121 tcgcaggtct acaacgacgc ccgcagcttg ctgacggtgg gtctgggcaa ggacatcgtc
    15181 ggcaaccccg tggtggccga tctggccagg atgccgcacg tgatggtggc cggcaccacc
    15241 ggctcgggca agtcggtggg catcaatgcc atgatcctga gcctgctgta caaggccgag
    15301 cccaaggacg tgcgcctgct gatgatcgac cccaagatgc tggaaatgtc ggtctacgaa
    15361 ggcattccgc acctgctggc gccggtggtc accgacatga agcaggccgc caatggcctg
    15421 aactggtgcg tcgccgaaat ggagcggcgc tacaagctga tgagcaaggc cggggtgcgc
    15481 aacctggccg gcttcaacca gaagatcgac gacgccaagg cgcgcggcga gttcatctac
    15541 aaccccttca gcctgacgcc cgaagacccc gagccgctcg agcgcctgcc ctacatcgtg
    15601 gtcatcatcg acgagctggc cgacctgatg atggtggtgg gcaagaagat cgaggagctg
    15661 atcgcccgcc tggcgcagaa ggcgcgcgcc gccggcatcc acctgatcct ggccacccag
    15721 cgccccagcg tggacgtgat caccggcctg atcaaggcca acattcccac gcgcatcgcg
    15781 ttccaggtca gcagcaagat cgacagccgc accatcctcg accagatggg cgccgaggcg
    15841 ctgctgggca tgggcgacat gctctacatg gccagcggca ccggcctgcc ggtgcgcgtg
    15901 cacggcgcct tcgtcagcga tgacgaggtg caccgcgtgg tcgaatacct caagagccag
    15961 ggcgagccca actacatcga aggcgtgctc gaaggcggca cggcggaggg cgatgcgggt
    16021 gccttcggcg aaggcggcga cgccggcggc gagaaggacc cgatgtacga ccaggcggtc
    16081 gaggtggtgc tgaaaaaccg caaggccagc atctcgctgg tgcagcgcca cctgaagatc
    16141 ggttacaacc gcgccgcgcg cctggtcgaa gacatggaaa acgccggact ggtgagcgcc
    16201 atgacggccg acggcaagcg cgacatcctg gtacctgcac gtccagaatg acgctggcgc
    16261 gcctgcagca agcgcgggca gctatttaat caggagcatc cgtcaggcct gcccaagcag
    16321 acggcggaac ttgtccagca actgctgcgg gtcttccatg cgctgtgggt tgacgggaat
    16381 gcagtccacc gggcagacct gcacacactg tggctggtcg aagtgaccga cgcattcggt
    16441 gcacttgtcg gggtcgatct cgtagtgctc ttcacccatg gcgatcgcct gattggggca
    16501 ttcgggttcg cagacgtcgc agttgatgca gtcgtcggtg atcatcaggg ccatgcttgc
    16561 gctcctgtgg gcgggttcga gggcgcgccg gacgcggcgc ccgatgtatt gataatgcca
    16621 cgctgcaccg gttgccgcgc atgagctatt tcctcttcaa acgcattctg acgctgctgg
    16681 cgacgctggc ggccaccagc gtgatcgtgt tcgcggtgct ggaggtgctg ccgggcaacg
    16741 ccgcccaggt gctgatgggg cccgacgccg atccggccgc cgtggccgcc aaggtggtcg
    16801 agctgggctt ggaccggcct ccgctggcac gctatgtcga ctggatgcgc ggcttgctgc
    16861 gtggcgacat gggcaactcc tacgcctatg gctcgccggt ggccgagctg atcgccgagc
    16921 gcctgctgct gaccgtgccg ctggcggtgc tggccatgac cctgacggcg ctgctggcgc
    16981 tggcggtcgg ggtgtacgcg gcgtcgcagc acaaccgtct gggcgacacc ggcctgatgg
    17041 cgctggcgca ggtgggcatc gccataccca atttctggtt cgcgatcctg ctgatcctgc
    17101 tgttttcggt caagctgcaa tggctgtcgg ccggcggctt cgacggctgg tacttcgatg
    17161 acggcgtgtg ggccgggctg tggcagggcc tgaaggcgct gatcctgccg gccgtggcgc
    17221 tggcgctggt gcaggcggcg atcctggcgc gcttcacccg ttcggcggtg ctggaggtgc
    17281 tgcgcgagga cttcgtgcgc accgcgcgcg ccaagggttt gagccgccgg gccacgctgt
    17341 ggggccatgt gctgcgcaac gccatgatcc cggtgatcac cgtgatgggc ctgcagtcgg
    17401 ccgagctgct ggccggcacc atcgtggtcg agagcgtgtt ctacctgccc ggcctggggc
    17461 ggctgatctt ccagagcatc gccaaccgcg acctgatcgt ggtgcgcaac tgcgtgatgc
    17521 tgctggccgc catggtggtg gtggtcaact tcatcgtcga tgtgctgtat gccgtgatcg
    17581 atccgcgggt gaaagcgagc gaggtatgag tcggccattc accacccaac cttcgtgcgc
    17641 catggacgcc cggatggggc agggcaggcg gaacttgcca gggcgattcc tggtttgccc
    17701 ggcgaaacca ttgcgcccca tgcgcatggg tgtcggctcg atcgaccgca gtggcgtgga
    17761 gcgatcgcgg tcatgagcgg cacccttcgg cttgccgccg ccgtcggcgc cgcccctggc
    17821 ttctgggcgc gcgcgcggcg ccaccctggc tttgtcatcg gcgcgctgct gacgctggcg
    17881 ctgctgctgt cggctgcgct gtcgtatgtg tggacgccgt actccgtcta cgaggtgcag
    17941 atggacgaca agctgctggg gcccgacgcg cgccactggc tgggcaccga cgccttcggg
    18001 cgagatgtgg tgtcgctgct gctggtgggc gcgcgcgcct ccatcctggt gggcgtgatc
    18061 gcggtcggca tcggcctcgt gttcggggct gcgctgggcc tgctggcgtc ggcgcggcgc
    18121 ggttgggtgg aagagctgat catgcgggtg gcggatttcg gtttcgcctt cccggccatc
    18181 ctgtcggcca tcatgctgac ggcggtgtac gggccgggca tcgtcaacgc catcattgcc
    18241 atcggcatct acggcatccc cgccttcgcg cgcatcaccc gcgccgccgc caacgccgtc
    18301 tggagccgcg actacgtgca ggccgcgcgc gccagcggca agggcgcctg ggccatcacc
    18361 tggcagcatg tgctgcccaa cattctggcg gtgctgatcg tgcaggccac catccgcttt
    18421 gccgtcgcca tcctggccga ggcggcgctg tcctacctgg gtctgggcac ccagccgccg
    18481 cagcccagct ggggccgcat gctggccgag gcgcagagcc tgatgttcga cgccccgctg
    18541 ctggcggtat ggccggggct ggcgatcgcg ctgtcggtgc tggggctgaa ccttctgggc
    18601 gacgggctgc gcgacgtgct ggatccgcgc ctgcaagggt cgcgctgaat cggtcatgca
    18661 agtggtaata ccaagtcgct acagtccagc catgaccctc accgtcaaaa gcccgcccta
    18721 ccttgagcag tcactgggcc agcaaagtgc ggccgagggg cgcagtctga gcgagctgtg
    18781 gcgcgacgcg ctgaccgccg atctggccag cgcccgccac gcccacgggg acgaggtgct
    18841 ggcgcacaag cgggcgggcc gcatcgcccg gcgctttgcc agaatgccgc tcaatccggc
    18901 tggtgcgtcg agggccgaag ccgccgcgcg ccgcgggctg cgccgcatcc tgggcatcga
    18961 cgccgcttgc atcgtgcgcc gcgacagccg cggtcgcccc ctgcccaacc tgctgtcgag
    19021 ctgaagccgt ggccctgctg caagccgaac acctgcgcgt cagcctgcgc acccaccgcg
    19081 gcagcgccac cgcggtgcgc gacgtcagct ttgcgctgga gcgtggcgac accctgggcc
    19141 tgatcggcga atcgggctgc ggcaagtcga tgaccgcact ggccctgatg ggcctgcagc
    19201 ccgacaacgc caccgtgcag ggcagcctgc gcctggacgg ccaggagctg ctgggcctgc
    19261 ccgaccgcga ctggcgccgc ctgcgtggca accgcatcgc catgatcttt caggagccca
    19321 tgacggcgct caacccggtg caccgcgtgg gcgcccagat cgccgagccg ctgcggctgc
    19381 accgcggcct gtcggcccag gcggcgcgcc agcgcgccat cgaactgatg gcacgcgtgg
    19441 gcatcccgga gccggcccga cgcatcgaca gcttcccgca ccagttctcc ggtggccagc
    19501 gccagcgcat catgatcgcc atggcgctgg cctgcgagcc cgacgtgctg atcgccgacg
    19561 aacccaccac cgcgctcgac gtcaccgtgc agcagcagat cctggcactg atccgcgagc
    19621 tggtggccga acgcggcatg gcgctggtgc tgatctcgca cgacctcggc ctgatcgcgc
    19681 agaacgtgcg ccgcatgctg gtcatgtatg gcggtgcggt ggtcgaaagc gggccggccg
    19741 ccgagctgtt ccggcagccg gcccatccct acacgcgcgg cctgctggcg gcccggccca
    19801 acctggcgca gcgcctgcat gcgcaccgca gcgaccgcct gcccaccatt gccggcagcg
    19861 tgcccgatct gttcggcctg ccgcccggct gcccgttcgc cggccgctgt gcctacaccg
    19921 agccggcgtg cctgaccgac ccggccccgc cggtggcgct gacggcgtcg caccgcttct
    19981 ggtgccggcg tgccgacact gttcttcatg ctcctgaatc aatagctggt ggcgcttgat
    20041 ggataagcgg gacaaccact ttttgcttga aacccgtgag ctcgagcggc gctacacgct
    20101 gccgcgcgag tcgctgttca gaacgccggg ccaggtgcat gcgctgcgcg gcgtgtcgct
    20161 gcaggtgacg gccgggcgca gcctgggcat cgtcggcgaa tcgggttccg gcaagagcac
    20221 gctggcgcgc ctggtgatgg cactcgagcg ccccaccgcc ggccaggtgc tgctgcaagg
    20281 gcgcgacctg cacgctctgt cgcccgatga gctgcgcgcg gcgcggcgcg acttccagat
    20341 ggtgtttcag gacccctacg gctcgctcga cccgcgccgg cgcatctggc gcaccgtggc
    20401 cgagccggcc gccgcgctcg aaggcgccga ccgcgccgag cagcgcgagc gcgccgccgc
    20461 catgctcacc gccgtcggcc tgcacgccga tgcgctcgac caatacccgc acgagttttc
    20521 cggtggccag cgccagcgca tcgccatcgc gcgcgccctg atcacccgcc ccaagctgat
    20581 cgtggccgac gaagcggtga gcgcgctcga cgtctcggtg caggcgcagg tgctcaacct
    20641 gatggccgac ctgcgcgacg cgcacggcgt caccttcctg ttcatcagcc acgacctggc
    20701 ggtggtggcc cacctgtgcc aggacgtggc ggtgatgcag ggcggccggg tggtcgaaca
    20761 tggcccggtg cgccaggtgc tgcgcgagcc gcgccacccc tacacacgcc agctgctcga
    20821 cgccgtgccc gtgtctgcgc tgctcgccgc cgtctgagaa ggtgcctgcg cccctcgtgc
    20881 ccgccgtgat cgccccggca cgcccgctcg gcgtggccaa ggttcgccgc cgcccctgcg
    20941 acggccgcgc ctggcgcctt ggtcggcggc gctggggcgc tccctttggg caccctggac
    21001 gcgcgcccgc cttctgagcg cgcgccgccg cgcctgcgct acgatgcacc tccatttcat
    21061 ccacctcgag gagatctgcc gatgaaacgc cgcgacttcg ccctcagcct gcccgcgctg
    21121 gccgccagca cccatctgcc ggcgctggcc caggccggca agagcaccat ggtgctgggc
    21181 atgacgctgg agccgccggg gctggacccg acggccggcg ccgcctcggc catcgccgag
    21241 gtggtgcact acaacatcct ggagacgctc accaagatca accccgacgg cagcgtctcg
    21301 cccctgctgg ccgagcgctg ggaggtatcg cccgacctgc gcaccatcac cttctatctg
    21361 cgcaagggcg tgaaattcag caacggcgag ccgttcaacg cccaggcggt gcgcttcagc
    21421 ttcacgcgcg ccgccggcga caagagcacc aacaaggaca agcgcacctt cgccgccatg
    21481 gaaagcgtca gcgccgtcga cgagcacacc gtggtgatca tcaacaagga gcccgacccg
    21541 gacttcctgt tctacatggg ccaggccacc gccgtcatcg tcgaacccaa gagcgccgac
    21601 ggcaacaacg cccggccggt gggcaccggt ccctatgagc tggacaactg ggccaagggc
    21661 tcgtccatca ccctcaaggc ctggcccggc tttcgcaacg ccagcgccat ccggatcaag
    21721 cgcgccacct tccgcttcat cagcgacccg gcggcccagg tggcggcggt gctggccggc
    21781 gacgtcgacg ccttcccgcg cgtggccccg cgcggcgcgt cgcagttcaa ggccaacccg
    21841 cgcctgcaga ccctcatcac cggctcgcgc gccaagaccg tgctggccat caacaacgcc
    21901 cgcaagccgc tcaacgacgt gcgcgtgcgg cgtgccatca gcatggcgat cgaccgcaag
    21961 gccgtcatcg ccggcgccgc cgacggctac ggcgtgccca tcggcagcca ctacgtgccc
    22021 ggtgcgcccg gctacgtcga caccaccggc atcaacccct acgacccgga aaaggccaag
    22081 gccctgctca aggaagccgg cgtcaccacg ccgctggagc tgtcgctggt gctgccgccg
    22141 ccgtcctacg cacgtcaggg tggcgaagtg atcgtggccc agctggccaa gatcggcatc
    22201 accgccaagg tgcagtcggt ggaatgggcg cagtggctgt ccaacacctt cggcggcccc
    22261 aagaattacg acctgaccat catcagccac gtcgaaccgt tcgatctggg caacttcgcc
    22321 aagcccgact attactgggg ctaccagaac ccgaagttca acgagctcta cacccggatc
    22381 aagaacaccc ccaccgccgc cgagcgcaac aagctgctgg gcgatctgca gaagctgctg
    22441 gccaacgacg ccgtgctggc ctggctgttc cagccgcagg gcgtgacggt ggccaacaag
    22501 cgcgtgcagg gcctgtggaa ggaatcgccg atcttcgtca acgacctctc gtcactcagc
    22561 tggggttgaa cgtttgctat taaatatgga gctgctggcg cacactggat aagcgccagc
    22621 ggctcttttt gcttgaactt cagaatgacc gaaccgttgc acgatctgtc cgcacacgcg
    22681 ctgctggcgg gctaccgggc gcgccacttc tcgccggtcg acgtgctgga cagcgtgctg
    22741 caccacgtcg aacgctggga gccgcacatc gccgccagct ggctgctgcg gcccgacgcg
    22801 gtgcgcgcgc aggcgcgtgc gtccgaggcg cgctggcggc gcggcgagcc ctgcggcgcg
    22861 ctggacggcg tgccggtcac catcaaggac aacatcgcca ccgccggcga cccggtgccg
    22921 ctgggcacgc gcgccgtgcc cctgctgccg gcaaccgccg acgcaccacc ggccgcgcgc
    22981 ctgcgcgaag ccggcgccat cctcctgtgc aagaccacca tgcccgacta cggcatgctg
    23041 tcctcgggcc tgtccacctt ccaccggctg tcgcgcaatc cgtgggacct ggcgctgacg
    23101 ccgggtggct ccagcgccgg tgccggtgcc ggtgccgccg ccggctacgg gccgctgcac
    23161 ctgggcaccg acatcggcgg ttcgatccgc ctgccggccg cctggtgcgg catcgtcggc
    23221 ctcaagccca gcctgggccg ggtgccgatc gatccgccct acatgggccg cgccgccggc
    23281 cccatgacgc gcagcgtgca ggacgcggcg ctgctgatgc aggtgctcag ccagcccgac
    23341 gcgcgcgact gcatgagcct gccgccgcag gccatcgact ggtcgctggc ctggcgccag
    23401 gacaagccgc tcaagggcct gcgcatcggc ctgctgctgc aagccggctg cggcctgccg
    23461 gtggagccgg ccattcgcgc cgccgtcgaa caggccgccc ggctgctcga agggcagggc
    23521 gccatcgtca cgccgtgggc acccttcatg acgcccgagc tgctggccgg gctggaccac
    23581 ttctggcgca tgcgctcgct gctcgatctg gacgcgctga gcgcgccggc gcgcgccagc
    23641 gtgctgccgg tgatccgcga ctgggccgaa agcgcgcgcg gcatgagcgg cgccgacctg
    23701 tttcgtgcct acgccgccac ccacgccacg cgcgtggcca ccagccaggc catgcagggg
    23761 ctggactacc tgatctcgcc ggtttcgccc aacgcaccgg cgccggccga atggccttcg
    23821 cccaccaacg acccgctgcg cgggctggag cacatcggct tcaccgtgcc cttcaacatg
    23881 ggcgagcagc cggccatctc gatcaactgc ggctacatgg cgtcgcccgc cacgccgggc
    23941 gccatgctgc cgatcggcct gcagatcgcc ggccagcgct tcgacgacct gggcgtgctg
    24001 caactggcgc gcgccttcga gcagttgcgc gggccgcagc gcccctggcc cgcgccgccg
    24061 gcgccctgaa gctccgcgct ggccaacacc ccgccggcga cgccgtcttg cccgccttcg
    24121 tagaatcgac ggcgtgagct ttccgcacaa cgccgacctg cactgccatt ccgtcgtttc
    24181 cgacggcacg ctgacgcccg aacaactggc cgcgcgtgcg cgcgccaacg gcgtggatct
    24241 gtggtcgctg accgaccacg acgaagtcgg tggccagcag cgcgcgctgg aggcggcacg
    24301 cgccgagggg ctgcgctacc tgaccggggt ggaagtgtcg gtcacctttg ccgacgagac
    24361 ggtgcacatc gtcggtctgg gcttcgacca caccgaccca cgactggccg aaggcctggc
    24421 cgacacgcgc gacggccgcg gcccgcgcgc cgtcgagatg ggcgagcagc tggcccgcgt
    24481 gggcatagcc ggcgcctacg agggtgcgct caggtacgtc ggcaacccgc gcctgatctc
    24541 gcgcacgcat ttcgcgcgct ttctggtcga aaccggcgtc tgccgcgaca ccgccgaggt
    24601 gtttcgccgc tacctggtcg agggaaagcc cggctacgtg ccgcaccgct gggcccgact
    24661 gggcgacgcc gtgcgctgga tcacccagag cggcggcgtg gcggtgatcg cccacccggc
    24721 gcgctacgac atctcgccga ccgtcgaata cgcgctgttc tcggagttca agcagcatgg
    24781 cgggcaggcg gtggaagtca tcaccggcag ccacaccgcc gccgaagccg ccaggtacgc
    24841 cgccatggca cgcgaattcg gtctggccgc ctcgcgcggc agcgacttcc acagccccga
    24901 cgagagccac accgacctcg gccaactgcc accgttgccg gctgacctga cacccgtctg
    24961 gaccttgctg gcaaaccgta ttcactaggc ttttgcgctt gtctggcaag cgccagcagc
    25021 tcctgaaatc atagtgaacg ccgttcatgg tccagcggct tgccgtgcat cccgacaacc
    25081 cacaccgcgc tcgccgcgcc atgcggcgtt gctcctgctt gcgggcctgc tgaacgcgcc
    25141 tgacgatcgc cagccccgtg ggacggtgaa ccgtcaacgg atggaggcgg ccagcagcac
    25201 cagcagcgcc cccgcgccgc cttccagcgg cttggcctgc acgaaggcca gcacttcggc
    25261 cttttgcacc agccagacct gcaccttggc cttgagcacc ggcgtgcggc cgggcgagcc
    25321 cagacccttg ccatgcacca cgcgtacgca gcgtagtccg gcgcgctggg cctcgcgcaa
    25381 gaactgcgcc agcgcctcgc gcgcctcgtc ggtgcgcagc ccgtgcaggt cgatctcacc
    25441 ctgcaccgcc cagtggccgc ggcgcagctt ggccgccaca tcggggccaa tcccgggacg
    25501 gcgaaagctc aggtgctcgt cggtgtgcag cagggtgctg gtatcgaact cgtcggagat
    25561 cgattcgcgc agcaccgcct gctcgtcgcg ctggcgctga cgcggctcgg gcgccaccgc
    25621 gggcggcgcg tgcaccaccc ggccatgcgc aggcagcggc tgcgtggcgc cgatggcgcg
    25681 ggcaaacagg tcctggtcgg cgcgggcgcg ctgctgcgcc gcggcgcgct cggcctgcgc
    25741 ctgcgccgcg cgcgcctcgc gctcggccag ctgggtgcgc agctgcttca gatcctgcag
    25801 ggagcgagct ttcacagcaa cccctgctcc gccatcgaca gcgcctgccc gggcgcgacg
    25861 atgatgtggt cgagcacgcg cacgtccacc agggacagcg cggctttcag ggtctgcgtc
    25921 agcgcctcgt cggcgcggct gggtgcgacc tggccgctgg ggtggttgtg tgccagcacc
    25981 acggcggcgg cgtggtggtg cagggcgcgc agcaccacct cgcgcgggta gacgctggtc
    26041 tgcgtcagcg tgccgcgaaa cagttcttcc agcgcaacca agcggtgctg cgcgtccagg
    26101 aacagggcgg cgaacacctc gtggggacgt tgccccaggt gcagctgcag gtactgcttg
    26161 acggcgctgg cgctgtccag agccggtttt tccttgagct gctcggccag cgcgcggcgc
    26221 gccagctcga gcacggcgat cagctcggcc cgcttggcgc tgccgcccag gcccttgaag
    26281 cgccccaggt cgtcggcgct ggcgtgcagc aggccggcga tgccgccaaa ccggtccagc
    26341 agctcctgcg ccatctgcag cacgccgcgc cccgcggtgc cggtgcgcag cagcagcgcc
    26401 agcagctcgg cgtcactcag cgcagcggcg ccgcgcgcca gtagcttttc gcgcgggcgc
    26461 gcgtcggcgg gaaggtcttt gagcgacatg cggggtccgg gggcgggggt tttctagaat
    26521 cggggcagtt tatcgggaca catccatgag cactgtcgag ccaggcgcct ttctgaccct
    26581 gcactatcgc ctgtcgggcc cgcagggcga cgtgatcaac accttcggcg ggccgccggc
    26641 cacgctgtcg ctgggcgcgg gcgagctctc gccggcgatc gagcagcgcc tgatcggcct
    26701 ggccgagggc gcgcgcgcca cgttcgagct gcccgccggc gaggcctttg gcgagcgcag
    26761 cccggacatg atccagtggc tggcacgcca ggatctggac gagctgggcg acccgcagga
    26821 gcgctacgcc gtgggcgacg tgatccagtt gcccacgccc gatggtcagg gtcagttcgg
    26881 cgccgtggtg caggcggtgc gcgacgatgg cgcgctgcgg ctggacttca accacccgct
    26941 ggccggccag ccggtgacct tcgaggtcca ggtgatcggg atcctgtgat gccgcagtcc
    27001 cacggccgct ccatcaccat cacgcgcaag gccgccgctg cggacggcga ggctgccgcg
    27061 gcgccgccgg tcgacgccca catcctgctg gccgagccgc gcggcttttg cgccggagtg
    27121 gaccgcgcca tcgaaatcgt cgaacgcgcg ctggtcaagt tcggcgcgcc catctacgtg
    27181 cgccacgaga tcgtgcacaa cacctacgtc gtcaatgacc tcaaggccaa gggcgcgatc
    27241 ttcatcgaag agctggccga ggtgccgccc ggcgccacgc tggtgttcag cgcgcacggc
    27301 gtgcccaagg cggtcgagcg cgaggcccag gcgcggggct ttcgcgtgtt cgacgccacc
    27361 tgcccgctgg tcagcaaggt gcatgtcgaa gtcgccaagc tgcaccgcga gggctacgaa
    27421 ttcatcatga tcggccacaa gggccacccg gaggtggaag gcaccatggg ccagttgccc
    27481 ggcggcatcc acctggtcga ggaggtgcag gacgtggcac gcatccggcc gacgcagacc
    27541 gagcgcctgg cggtggtgac ccagaccacg ctgagcgtgg acgacgcggc cgaaatcacc
    27601 gccgccgtga aggcgcgctt tcccatggtg cgcgagccca agcagcagga catctgctac
    27661 gccacccaga atcggcagga cgcggtcaag ctgctgtcgc gcgaagtgga cgtggtgatc
    27721 gtggtcggca gccccaccag cagcaacagc aaccgcctgc gcgaggtggc gctcaagctc
    27781 ggcaccgccg cccacatggt cgacagcgcc gacgacctga agcccgaatg ggtgcagggc
    27841 cgcgcgcgca tcggcctgac ggccggcgcc tcggcgccgg acgtgctggt gcgccaggtg
    27901 atcgagcggc tgcgcgcgct gggggcggtg tccgtgcgcc agctcgccgg catcgaggaa
    27961 accatgaaat ttcccttgcc caagggcctc aagctcgaca gcgccagcga agacggtctc
    28021 gcgcatctgc gctgaaaact atgattttga tagctgttgg cgcttgccag gcgggcgcaa
    28081 acggctgctt ttgctttgaa tcgggggcgg ccggggcgcc gccgcgcgcc tctacaatcg
    28141 gaccatgctt gatccactgc tgcttcgcaa agacctcgat ggcgtcgtcg cacgcctgct
    28201 gacccgcaaa aatccccaac cctttctgga cgtcgcggcc ttccaggcgc tggaagccga
    28261 gcgcaaggcc atccagaccc gcaccgaaga gctgcaggcg cagcgcaaca gcgccagcaa
    28321 gcagatcggc cagcgcaagg ccaagggcga gagcgccgac gatctgatgg cgcaggtggc
    28381 gtcgatcaag gacgagctcg acgcttccgc cgcccggctg gagcggatcc aggacgagct
    28441 gcagacgctg ctgctggccg tgcccaacct gccgcacgac agcgtgcccg tgggcgccga
    28501 cgaagccgcc aaccacgagg tgcgccgctg ggcgccgcat gggctggcgc cgcgcagctt
    28561 cgacttcgcg gtgcgcgacc acgtcgagct gggcgagccg cttggcctgg acttcgagac
    28621 cggcgccaag ctggccggca gccgctttgc cttcatgcgc ggcccggtgg cgcggctgca
    28681 ccgcgcgctg gcgcagttca tgctggacgt gcagaccggc gaacacggct acaccgagtg
    28741 ctacacgccc tacatcgtca atgccgagac cttgcgcggc accggccagc tgcccaaatt
    28801 cgaagccgac ctgtttgccg ccaagaaggg cggccaggag ggcgagggcg agccgctgta
    28861 cctgatcccc acctccgagg tctcgctcac caatctggtg cgcggcgaaa tcctggccga
    28921 agccgatctg cccctccaac tgaccgcgca cacgccgtgc ttccgctccg aagccggcag
    28981 cgccgggcgc gacacgcgcg gcatgatccg ccagcaccag ttcgacaagg tcgagatggt
    29041 gcagatcgtg catcccgagc gcagctacga ggcgctggag cagatggtca cccatgccga
    29101 ggccatcctg cagaaactgg agctgcccta ccgcgtggtg ctgctgtgca ccggcgacat
    29161 gggcttcggc gccagcaaga cctacgatct cgaagtctgg gtgccggcac agaacaccta
    29221 ccgcgaaatc tcctcggtca gcaactgcga agccttccag gcgcgccgca tgcaggcgcg
    29281 cttcaagacc gcgcagggca agaacgagct ggtgcacacc ctcaacggca gcggcctggc
    29341 ggtgggccgc gcgctggtgg cggtgctgga aaaccaccag aacgccgacg gctccatcgg
    29401 catcccgcgt gcgctgcagc cgtatctggg cggcatcgag cgcctggcgc cctgaggcgg
    29461 gtgcgcggca cctgctagaa tctgcctttc gacacgaagc aggagaggtg gcagagtggt
    29521 cgaatgcgcc ggactcgaaa tccggtatac ggtttaccgt atcgagggtt cgaatccctc
    29581 cctctccgcc acagacaaag aaagcccgct atgcaaacga tagcgggctt tttcttatcc
    29641 tgatccctgt cggcaatttg tgggccgttt gctcaaatct gccaggaact tgatcggttt
    29701 gcgggaatcc cctcaggtga tccagcacgg tgccgctttg ggtgatgtgg atgcgttctg
    29761 cgggcttgtt gcttgcgaac tccgcaggtg ctgtgcgaac cgtgtggcgg catcaccgga
    29821 agacgtgggg cgcaagaaac tgacccgtca tcagcatgcc cgcgcgtgcc cggttctgtt
    29881 cggcaatcca tggaatcccc cggcgccccg aaagggcgaa tcaataggaa ccacaccaaa
    29941 cagggccaag gtcacttcgt ctcatagcgg tcgatgcctg gaccaccatg gaacgcgtgg
    30001 accggcctgg cgtgctggcc gccgccggtg gcgccacggt catctgggtg gactttccgg
    30061 cgcagaaggc ggcgccgctg cccgactgga tgcgcgttct ggtggtgtag ccaccgtcca
    30121 ttgcatgcgc gcctcaaagc cgccgctctc gtgcggcagg ggtgatgtca aggtgagctg
    30181 ggcatcgtgc ttggctgcga tggtggccac gatggacagg cccagcccgt agcccgcgcg
    30241 gtccgcactc tggcgcacat ggcgctgctg cagggacttg agccggtccg cgctcacgcc
    30301 ggggccgtgg tcgcgcaccg cgagcatggg gccggggccg acgaggatct ccaccgggcc
    30361 cggggccgcg tagtggagcg cgttctccac caggttgcgc agggcgatgg ccagggcgtc
    30421 gaagtcgccc agggccgtgc aatgttcccc atcggtggcc gttatcaact ccagccggtc
    30481 ggccgcgcgc tcgtcctgcc agaactcctg cgccaccgtg gcggcaagcc gcaccagatc
    30541 gacgggttcg cgcgagagcg gggcgctgga ttccgcgcgc gagagctgca acagcttttc
    30601 ggtgcgctgg ctcagggtgc gcagcgcatc gaccgcggcc tgcacgtctg cgcggtgcag
    30661 gccatgctcc agcgcggtct gcagccgcag ccgggccgtg gccagcggtg tgcgcagctc
    30721 gtgcgcggcg ttggcggcca gggcgcgctc cacgtccagc gcctggtcca ggcgctgcag
    30781 caacaggttc acgtcctcgc ccacggcgcg cagttcggcc ggcaggcccg tcagcgtcag
    30841 cggccgcagg tcgccgccgc cgcgccggcg gatttgccgc tgcagccgct ccagcacccc
    30901 gagctcgcgc gtgacgacgc cgggcagggc ccaggccagt gccgccagcg cgagtacggc
    30961 cgccaccagc agcaccagca gcgtctcgcg ccattcgtgg ttgcgctccg acaatgggtc
    31021 ggccagcaga aaaaaaagcg cctgctccgg gtgctgcgcg acatgcacgc gccattggtt
    31081 cgtgtcgtga aagccgggct cgagcggcac ggccagcgcc tgcgccggcg cctgacgcga
    31141 gcgcagcagc agccggccct gggcgtcgac aagctggtag acgagaggcg cgtcggcaaa
    31201 cagcggatcg tctgcgatca cggcatcagc acgcggcgcc tggcccgccg cacccgccgg
    31261 cgcgccgcgc tggagctgga acatggccac gtcgaacatg cggtgcgcgc tctcggccag
    31321 ctcggcatcg aagttctctt cgatctcgtg gttcaagtaa taggcgacgg ccagcacgca
    31381 cagcagccac accagggcca gccgcagcac cagggccctg gtcaggcgtt gcgcgagcgt
    31441 gctggccctc atgcggcggc cggctcgctg tgcagcctat agcccaggcc gcgcactgtc
    31501 tcgatgtgcg tgcgcccgag cttgcggcgc aggcggctga tgaacacctc cagcgtgttg
    31561 ctgtcggtct cgtcgcccca gccgtagagg gcgtcctgca ggttttcgcg cgtgtggatg
    31621 cggccggggc gcgtggccag cacgcgcagc agggcccatt ccttctgcgt cagcgcaatg
    31681 ggcacgctgg cgcggcgcac ctgctcgcgc gccaggtcga tctgcaggtc gcccagcacc
    31741 agcaccggag tctcagccgc gctgcggcgg cgctccaccg cgcgcagccg cgcaagcagc
    31801 tcgccggggt cgtagggctt gatgaggtag tcgtccgcgc ccgcgtccag gccgcgtatg
    31861 cggtcgctga cctgatcgcg tgcggtgagc acgatgacga tggggcgctg ctgcagcgcg
    31921 cgcaactggg gcagcagggc caggccgtcg ccgtcgggca gatgcaggtc gagcagcacg
    31981 gccgcgtatt gcgcgccatg cagcgccgcc tcggcctcgc tcaggctggc cgtggcatcg
    32041 accacgaagg ccttggcgcg cagatagccg cacacggcgt cggacagggc cagatcgtct
    32101 tcgaccagca gcacacgcat ggggactccg tagatttttc cgatgcggaa agtgtagtgg
    32161 gcggggcgtt catggcgcgt tcaggatggc ggcataggct tgggcccatg ttcgatgttc
    32221 tatggcgcag actggcgctt gtcttgctgc cgctcgtgcc gctgctcctg tgggatgcgt
    32281 cgggtctgga tcttccgctg gcgcggctgt tcggctcggc ccagggcttt gcctggcgca
    32341 gccacccaag ctatgtgctg ctgctgcacg aggtgccgcg cgccctcagc atggcggcgc
    32401 tgctggcgct ggcctggggc gcatggcggc cgtgggggtt tctgcgcacc atgggggcgg
    32461 cggacaggcg ccaactcgcg ttcagcgtgg cgctggccat gctgtccatc acggcgctca
    32521 agcgcctggc gacgacgagc tgcccctggg acctggcgga gtttggcggt gtggcgcact
    32581 atgtgtcgca ctggatctgg ggcgtgcgcg atggtggccc gggccattgc tttccggcgg
    32641 gccatgcatc ggccgggttc ggctacgtgg cgggctggtt cgtgctgcgc cgcgccgtac
    32701 cgcgcctggc aaccggctgg ttgttggcga gccttgtcct gggctgcgcg ctgggcctgt
    32761 cgcagcaggt gcgcggcgcg cactacatga gtcacacgct ctggacggca tggttctgct
    32821 gggccttggg cctgctggtg gacgaggccg tgcagcgctg gcgcagcaga acccgtgcgc
    32881 gggatacaaa cctgaacgaa acctgagcgc ctcgttcagc cagccttcag agcacgacag
    32941 cacgatgggc ccatgctcag tcttgcctct gccaacaccg gctccccatc ctctcccacc
    33001 tctttcaatt tgctccagcc cctgcgggcc ttgctgcgcc gcgtggacgc ctggatggcc
    33061 acgcccttga cggcccagca gctggtgctg cgcctgtcgc tctatctggt gcttgcggcc
    33121 aactggccgc tgtggctgca gatcatgcgc attggcggcg cgcccagcca gtacctgcgc
    33181 tccatggcgg cgctggcggt gctcatcgtc tgcggcatgg tggccatttt tgcctttttt
    33241 gcctgggcgc gcggcatgcg gctgctgtgg tggctggtgg tgctggtcgc tgcgctggcg
    33301 cagtactaca tgctgaccta ccaggtggtc atggatccgg gcatggcggc caatgtgctg
    33361 cagaccgacg cccgcgaggc ggccgacctg ctgagcctgc gcatgctgct gaccgtgctg
    33421 gccgtgctcg tgctgcccag ctggtggctg ctgcgcgtgc gcattgtggc catggggccg
    33481 ttggcccagc tgtggcgcaa cctcgtgatg ctggtgctgg ccctggccgt ggccgcaggc
    33541 acggtggccg ccaccgcacg cgacctggcg ccgctgatgc gcagccaccc gcagctgcgc
    33601 tatctgatga acccgctggc cagcctgtat tcgacgcccg tggcggccct gcggccggtg
    33661 tttgcgcgca gccgcaccct ggtgcccatg acccaggggg ccgcgctggg gacatcctat
    33721 gcggcgggcg ccaggcccat gctgttcgtg ctggtggtgg gagagacggc gcgcgcggac
    33781 catttcggca tcaacggcta tggccgcgac accacgcccg agctcgcgcg ccgcgacgtg
    33841 ctgagctggc gcgaggtgcg ctcctgcggc acgagcacgc tggcctccgt gccctgcatg
    33901 ttctcgccgc tgggcaagga cgcgttcgag tcgcgcgaca acgaatacga gaacctgctg
    33961 gacgtggccc agaccgccgg catggccgtg ctgtggctgg acaaccagtc cggctgcaag
    34021 ggggtgtgcg atcgcgtggc gcatgccagc gcgtccgagg ctctgccgcg cgatgctcag
    34081 gccgccctgt gccccggtgg cgaatgcggg gacgacgccc tgctgcacga cctggacgcg
    34141 cgcctggccg ccttgcccgc ggcgcagcgc gagcgcggcg tgctgctggt gctgcaccag
    34201 atgggcagcc atgggccggc ctaccacaag cgctcgcacg gcgccgccaa gcgctttttg
    34261 cccgaatgcc gcaccgaggt gctggccgat tgcgcccagc aggagctggt caacgcctac
    34321 gacaactcca tcgcggcaac ggacctcttc ctgggcaaga ccatagactg gctgcaggca
    34381 cgctcggcgc gctacgacac gggcctcttg tacctgagcg accacggcga gtcgctgggc
    34441 gaatacggtc tgttcctgca cggcatgccc tacaacatgg cgcccgacgt gcaaaagcat
    34501 gtgcccatgg tggcctggct cgatgacgcc ctgctgcgcc gtgagcgctt gtcggacgcc
    34561 tgcctgcgcg ccggcgccaa tgcgccgctc acgcacgaca acctctacca caccatgctg
    34621 ggcctgctgg atgtgcagtc gcccacgtac acaagggcgc tcgatgcctt tgccgactgt
    34681 cgcagggtgg cgctggccgc gcgttgagtc acttgcgttg ggtgggttgc gcttgcgccg
    34741 ggcgtttccc tgggtcatgg gcggttcagg ccggtacggc gtgacggcac gcgcaacgga
    34801 ggaatcttcg cttgctgacg tgcgcgcctc aggtcatggc tgccttgcaa ggcagttcac
    34861 atgttggtag gtctcccaag cccggggccc ggccgggcct ccatgcgcgg cgccaggcgg
    34921 acggaatcgc cggtggtcgg atcgagcggt cgcgatggcc gtacagtggc gggcatgaac
    34981 ctcgaccacg gtatccacgt catcgacacc ggcttcgtgc gcccgcacct ggacgcggcc
    35041 tatctgatcg tcgagcgcgg gcgcggtgcc ttcatcgact gcggcgtcaa ccattcggtg
    35101 ccacacctgc tggcggcgct cgcgcaggcc gggttgacgc cgggcgacgt ggactggctg
    35161 atcctgaccc acgtgcatct ggaccacgcc ggcggcgccg gcgagctgct gcgccagttg
    35221 ccgcaggcgc gcctggtggt gcacccgcgc ggcgcgcgcc acatgatcga ccccagccgc
    35281 ctgtgggcgg gcgcggcggc cgtgtatggc gaggccgaga tgcagcgcag ctacggccgc
    35341 ctgctgccgg tgccggccga gcgcgtggtg gcggcgcctg acggccatgt ggtcgagctg
    35401 gccgggcgcc cgctggtgtg cctggacacg cccggccacg cgcgccacca caacgccatc
    35461 cacgacctgg cctcgggctg cgtgttcagc ggcgacacct ttggcgtgtc ctaccgcgaa
    35521 ttcgacaccg agcagggcgc cttcattctg cccaccacct cgccggtgga gctcgatccg
    35581 ccggcactgc accgctcgat cgaccgcctg ctggcgctgc agccgccggc catctgcctg
    35641 acccactacg gaccggtggc gccgcccgcc cgtctggcgc aggatctgca cgcgcagctc
    35701 gacgccatgg tcgagctggc ccacgccgcc gcgccgcagc cgtcggacga gcgccaccgg
    35761 agcctgcgcg ccgggctgct ggagctgtac gcccgccgcg ccgcgcagca cggttggcgc
    35821 ggtggccggg agcgcctggc cgagctgctg gcggtcgaca tcgagttgaa cgcgcagggg
    35881 ctgggtgttt ggctggatca gtcgacgcgg atgacgaaag accctaaaaa cgacagttga
    35941 ccgcttgtca actttgggca ggtcgcatcg atgcggggtc tggcggctgc gatggacttc
    36001 acctcccggg tgaacacgct acgcccccgc ccgcaccgcg ctcgccgcat ggcgcggcga
    36061 tcacggcacg ggcgggcgcg tccaactgcc gtttccagga tgacggatga cctcgcggcg
    36121 cttcgatggg tcgtggcgcg tgggtcgtgg gcgcgtcagg ccggggatgg tgagcgcggc
    36181 gtacccgtgg ctgggccctt cagatccagc ccagcattcc cagcagcgcg ccggcgccga
    36241 tcagccacag caggtgcagc cgggtgcgcc acaccagcac ggtggtggcg gcggtcagca
    36301 gccacagtgg ccagtcgcgc gccggctggc cgtgggcggt gctcagcagc cagccggtgg
    36361 ccatcagcag ggcgatcacg atgggcgcca ggccggcctt gaacgcgcgc acggcgcgca
    36421 gctcgcgccg ctggtgcgcc cagcgggtgg cgctgtaggt cagcacgccc gagggcagca
    36481 gaatgcccag catggccacc gccgcaccca gcagggccag cccctgcgcg tgccagccgc
    36541 cggcggggcc gccaccggcg ttcagcccca cgtgccagcc caccagggcg acgaacagca
    36601 cgttgggccc gggcgcggcc tgggcgatgg cgatcgaggc gttgaactgc gcatcgctca
    36661 gccagtgctg cgagcccacc aggtagcggt gcatgtcggg cgcggtggtg atggcgccgc
    36721 ccaccgccag cagtgacagc gacagaaaat gcagccacag cgtcagccag tcggccgcgc
    36781 tcagggcgtc ggccgggttc atggcagccg cctccaggcc cagacgcagg ccgcgccgcc
    36841 cagcaccagc agcacgggcc ccagcggcag ccgcagcagg ccgatggcga cgaaggtcgc
    36901 cagcgccagg ccgatgcagg ccggcatgcc cagggggttg gcgcgcagcg caccgatcag
    36961 cttgatgccg gtgcccagaa tcagcccggc cgccaccgcg cccatgccgc gcagtgcgcc
    37021 ctgcaccagc gggacgtcgg cgacgttgct gtagagcgca gccagcagca cgaccgccag
    37081 cgtgggcagg gtcagcatgc cggccagcgc cgccagcgcg cccgacaggc cgaaatggcg
    37141 gtcaccgatc atcagcgaca ggttgaccac gttggggccc ggcatgatct gcgccaccgc
    37201 ccagtcttcg acgaactgct cgcgcgtgag ccagcgcttg cgctccacca gctcgcgctg
    37261 caccaccgcc agcacgccgc caaagccctg cagtgccagc cagctgaagg accagaacag
    37321 gtcggcgcgc gaccgtggcg cgccgagggt gccgtcaccg gcggcagcgg ggggggtggc
    37381 gctcataccg gttccggttc ggagcaagga ttcacgtccc tcgcgccatc atcgacgcgc
    37441 agcgaggtca tgggacatgg atcatcgtga atgcgagccc tcatcagtcc agcaggtcca
    37501 gaatgttctc gaccggccgc cccagccgcg cgcccctggc cgtgaccacg atgggccgct
    37561 cgatcagcac cggcgtggcg tgcagggcgg cggccagttg ggcgtcggtg agcgttgcat
    37621 cgtccaggcc ctgcgccagg tactcgggct gcttggtgcg caccagggcg cgcagcggcg
    37681 cgccgctggc gcgggcgatg gcctgcaact cggcggcgct gggcggcgtc ttgaggtact
    37741 ggacgatggt gggctcgatg ccgcggctgc gcagcagctc cagcgccttg cgcgaggtgc
    37801 tgcaatgggg gttgtggtag atcgtgacgg tggccatgcg ggtgctccga tagggctggg
    37861 ctgactacag taatgatagc ttttggcgct tgctggataa gcgctggagc cgtgtttcat
    37921 tcataaatca tgaatggcgt gcgggccgcc cgccaggcct gctcgtcggc gcgcgtcagc
    37981 gcgtcgagct cggccggcgt ggccgccgcg tcgtccaccc actggcgcag cagcggcccg
    38041 ccgttgatga cgtcgatggc cagccgggtt tcgtactcgt aaggaaagtc gcgccacagc
    38101 gggtagtccg gcgcctgccg ccgcagcgcc ttgaaggcca gcgcctgcag gcgccagggc
    38161 tgaaaggcgg tgtggtcgta gtgcgtcgcg tcctcggtgt ggatctgcac gccggcgcac
    38221 atttgcccgg cgtgcttgtg aaaggtcggc tcgaagccgc attcgcgcag ccggcagccg
    38281 cgcagccagt cgggcgccag tgcgcgcatg tcggccagca ggcgcgcggt gtcgatgtcg
    38341 ggcgcaccaa acagctccag cgggcgcgtg gtgccgcggc cttcgctcag cgtggtgcct
    38401 tccagcatca cggtgccggc gtaggcgcgc gccatccaca ggttgggcgc gttggggctg
    38461 gggttgaccc aggtgcgttc gagcggccag ccgtggccgg gcgcggcgtc gggccgccag
    38521 ccttccatgg cgatgacgcg gtattcgacc tgcagcccga gctgcgcgat gaaccagtgg
    38581 cccagctcgc ccagcgtcag gccgtggcgc atcggcagcg ggccggcgcc gacgaagctt
    38641 tcccagccgg cgcgcagcgt cagcccctcg accgggcggc cgaccgggtt ggggcggtcc
    38701 agcacccaca ccgccttgcc gtgcgcggcc gcggcttcca gcacgtagcg cagggtggtg
    38761 atgaaggtgt agatgcggca gcccaggtcc tgcagatcga ccagcagca
//
LOCUS       DAOOOG010000008        36830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_890_length_36830_cov_27.229096, whole genome shotgun sequence.
ACCESSION   DAOOOG010000008 DAOOOG010000000
VERSION     DAOOOG010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 36830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36830
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_890_length_36830_cov_27.229096"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..2135)
                     /locus_tag="PK242_01495"
     CDS             complement(183..2135)
                     /locus_tag="PK242_01495"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOZ92728.1"
                     /translation="MHVRAFLDQSIRFSTLALCALGTLLALVWMALAGPGPWSTALML
                     LCAVVVGVGVYDLQQTRHAILRNYPILGHLRFALEFIRPEIRQYFIEGDVEAGEPFTR
                     AQRSVVYQRAKGVPDVRPFGTKLDVGANGYEWINHSMQTTHIESHDFRIWIGGQPGVP
                     REGVAPCTQPYNASVFNISAMSFGALSANAILALNLGARMDGFAHDTGEGGISRYHRQ
                     HGGDLIWEIGSGYFGCRNDDGSFSPERFARQAVDPQIKMIEIKISQGAKPGHGGMLPG
                     AKVTPEIAAARGIPVGQDCVSPSAHSAFSTPIELMHFIARLRQLSGGKPVGFKLCIGH
                     IWEWFAIVKAMLETDITPDYIVVDGAEGGTGAAPIEFADHMGAPVQEGLNLVSNTLIG
                     VNLRHRIKIGAAGKVINSFDLARMFALGADWCNSGRGFMMALGCIQAQVCHTGFCPTG
                     ITTQDPLRERALVVADKATRVAQYHANTLHALKELLQAAGLMHPDQLYTHHIVRRIDA
                     TRVRLLSAMMPTMRFGAILNDLEHQHNVYRLYWPLADAHSFSLHPPTAEELAASPGIR
                     PTIAGRPASAVQIAEELTRRRLPTPAPVSHLVPEQARTFHSSAVSPSQVPPEWTGSGS
                     DLLAAAMQLQDEHAHDAQAHADDRRP"
     gene            complement(2308..4467)
                     /locus_tag="PK242_01500"
     CDS             complement(2308..4467)
                     /locus_tag="PK242_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOZ92729.1"
                     /translation="MELQTLRYAIEDTASGPVATITFDEQGSPVNTMCRQWQDDLVAV
                     TDQVLHDKARLRGLILASAKKTFFAGADLKGVMRTTQADATSVFTEIERIKKCFRSLE
                     TLGIPVVTCINGSALGGGMETALVGHHRIAVDDPKLQLGLPELTLGLIPGGTGITKFT
                     RLLGLMGAQPYIMESKLFGPREALEIGVVHELVASSDELRPRALAWIAEHPQAAHPWD
                     VKGYKMPGGTPANPKIAGALVVAPAMLKLKTRGRYPAPEAALAAMVEGAQVDYDTASR
                     IESRYLASLMTGPVARNMINTFFFNLNAIKSGQSRPQVATRFKPQKVGILGAGMMGAG
                     IAWAQASRGIATVLKDVSPDKAEQGKAYSAKLTQKRVDKGRMSAPEQQALLARITPTA
                     DAADLKGCELIIEAVFEQRELKAKVTQEAEPMLAEGGFFASNTSTLPISGLARASRDA
                     RKFVGIHFFSPVDKMKLVEIIRGRQTDDETVARAYDYVLALGKLPIVVNDSRGFYTSR
                     TFGTFVMEGAAMLGEGIPAAAIENAAVQAGLPVGPLAVLDETALSLSVHVLDQTRADF
                     AAEGKTYQATPGELLVERMVKELGRPGRAGGGGFYDYPQGGRKHLWPQLKALFEKPDT
                     AWSVQDIQDRLLYRQSVETARCLAEGVLTSVHDANIGSIFGIGFPAWTGGAMQFIYGQ
                     GIEAFEQRCAALAARHGDGFRITDEVAATLRRLQPRY"
     gene            complement(4563..5768)
                     /locus_tag="PK242_01505"
     CDS             complement(4563..5768)
                     /locus_tag="PK242_01505"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOZ92730.1"
                     /translation="MTEAYVFDAVRTPRGKGKKDGSLHEVKPIDLLAGTLEALRTRHD
                     FDTAAVDDVVMGIVSPIGEQGSVLPKVAALKAGWDWQCAGVQLNRFCASGLEAVNLAA
                     QKVRSGWEDLVVAGGVESMSRVPIGSDGGAWAQDPATNSATDFVPQGIGADLIATLEG
                     FTRQDVDAYAVESQQRAARARASGCFAGAVVPVKDFLGQTILSEDEFIKPRTTLEALA
                     GLKPSFEQLGAMGFDAVAIGRYPQVERIDHVHHAGNSSGIVDGAAAVLIGSEAAARTH
                     GLTPRARIVATAVSGADPTIMLTGPMPATRKVLAKAGLTIDQIDLFEVNEAFAAVPMK
                     FMKAFGVPHDKVNVNGGAIAMGHPLGATGAMILGTLIDELHRRGLRYGLATLCVGGGM
                     GIATIVERL"
     gene            complement(5910..8678)
                     /locus_tag="PK242_01510"
     CDS             complement(5910..8678)
                     /locus_tag="PK242_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HOZ92731.1"
                     /translation="MLTDLQLAQIMRHASTRRRAQCLDPLNRAMQAHRIDANLQRIAA
                     FLAQLGHESGELQFLEELWGPTPAQKRYEPPGELARRLGNTQRGDGRRFKGRGPIQIT
                     GRANYARYGELLGLDLVGQPEQAADPEVGFEIAALFWDRNGLNALADAGDFETITRRI
                     NGGLNGLADRQRLHALALQVLQGGLPEPVGSSASTLPVAAAAARRRARTRAGSAALRL
                     ARGHEAMVTDQAERPDALAATRTPRGRRPATGAMPSRRLDARPDTLDFRDQMFMPTLV
                     EVPSHVALGEYLEWQVPVLDQGTEGACTGYGLATVAHYLLRRRRVVADATPVSPRMLY
                     ELARRYDEWPGEDYDGSSARGAMKGWYKHGVCAETLYPSRPRGGRRTRPVPPAGGLDE
                     ARVSDALRRPLGAYFRVNHKDLVAMHSAIAEVGVLYASCTVHAGWSAVGADGVIGPST
                     RILGGHAFAIVAYDERGFWVQNSWGPGWGRNGFGLLSYDDWLEHGTDVWVARLGAPIT
                     LQRGASTASIHARSAAQSVGYSFAELRPHIVSVGNNGTLKAGGDYGSTPEELQRVFEQ
                     DIPRVSAGWAVPRVLLYAHGGLVSEQAGVQRLAEYRPLLLDAQVYPLAFIWRTDYWTT
                     ITNIIKDAVSRRRPEGLLDATKDFMLDRLDDALEPLARRLTGKSAWDEMKQNALAASA
                     RGGAAERVADLLLALRARLPGLEVHLVGHSAGSVLLAPLVDLLGARGLVVKTCTLWAP
                     ACRVELFTRHYQPALESRQIEKMALFALTDKVEQDDHCAGIYHKSLLYLISHAFEERP
                     RIPLFRDGEPFLGMERHIDRALMQRLGVDLVLAPNTEPADSLSASGAMHHGDFDDDAR
                     TVTATFRRVAPSVAAARPPASAPAGPRQAGADLLSHDLGALDPLFHRSAAALREQRQW
                     IDLRTRAA"
     gene            9122..10594
                     /locus_tag="PK242_01515"
     CDS             9122..10594
                     /locus_tag="PK242_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOZ92732.1"
                     /translation="MYKHCFRMACCAAALLTAGCASMAPPHERPIVAMPPTFASATPA
                     DVGTQTVAALGWRSYFTDPALTRLIEMALAHNPDLRTAVLRVQEARATYGVQRSAQLP
                     TVGVGLDASRTRTPGDLSPSGSAVTANQFQASVGISSWEIDFWGRIASLNEAALQSYL
                     ATDAARRAASVSLIAQVADGWLGLREAQERLRLAQASAANQRESLRIFTRRLEVGAAS
                     YLELTQVQTLLVQAELLVAQMQQQRDARRLSLNTLLGDLSDGSTVATEANTPLDDTMI
                     APVAPGLPSDLLTRRPDVLAAEHRLQGANANIGAARAAFFPQISLTAFGGTASTELER
                     LFRGANRVWGFSPSLSLPIFDAGRKRANLALAEVRKNLAVVQYEQTVQNAFREVADAL
                     SAQHWALRQVEVHQRALAIERERARLSRLSYDHGASSFLEVLDAERNLISAEQQLVQT
                     RRALLSSRVALYAALGGGSHDVDETPPGPTSASPANFTRR"
     gene            10600..11673
                     /locus_tag="PK242_01520"
     CDS             10600..11673
                     /locus_tag="PK242_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOZ92733.1"
                     /translation="MISSIKNKLLAVLAIAALAAGGWYAWQASTAKVDKPSFVAGNGR
                     IEATEVDIATKLAGRVNDILVTEGAFVKAGQVLATMQVQTLEAQLREAQAQRAQAVTS
                     VAAAEAQLAMRHSDKAALAAQVHQAAAERNAAQRRLARSETLVREGATSVQALDDDRT
                     RMESAVAAVAAAQAKVTSADASIGAAKAQVVSAQSQVRALDATLERIQAEIADSQLKA
                     PRDGRVQFVVAQPGEVLGAGGRVMNMLDVSDVYMTFFLPTEAAGRVALGADVRLVLDA
                     APDFVIPAKVSFIASAAQFTPKTVETASERQKLMFRVRARIDRQLLLEHLEHVKTGLP
                     GEAWVRLDPAQDWPTRLALKARP"
     gene            11670..14414
                     /gene="rbbA"
                     /locus_tag="PK242_01525"
     CDS             11670..14414
                     /gene="rbbA"
                     /locus_tag="PK242_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HOZ92734.1"
                     /translation="MTGLANAPVVRVEALRLRHGKVQALAGVSLELPAGRMVGLIGPD
                     GVGKSSLLSLLAGARAVQEGRVEVLGGDMASKRHRDIVGPRIAYMPQGLGKNLYPTLS
                     VEENLQFFARLFGHDRAERRRRIDELTQATGLHPFLDRPAGKLSGGMKQKLGLCCALI
                     HDPDLLILDEPTTGVDPLARAQFWDLIANIRTGRPGMSVVVATAYMDEAQGFDWLVAM
                     DDGRVLATGTPAELLERTGSDALEAAFIALLPESRKRGHAPVVIPPLQADADDIAIEA
                     KDLTMRFGDFVAVDRVSFRIRRGEIFGFLGSNGCGKSTTMKMLTGLLPASAGQAWLFG
                     REVDPRDIATRNRVGYMSQAFSLYSELTVRQNLVLHARLFHVAETDIPARVAEMVERF
                     GLGPVLDELPESLPLGIRQRLSLAVAMVHKPELLILDEPTSGVDPVARDAFWRLLVEL
                     SRRDRVTIFISTHFMNEAERCDRMSMMHAGKVLDSAAPAKLVEKRGAATLEQAFIGYL
                     VEAGGATPASAAPIGAVAAAAPSREAAAHARRPFSLQRLYSYLWREALELQRDPVRAT
                     LALAGSIILMIVMGFGIGNDVAGIRYAVLDRDQSALSNSYLLDIGGSRYFQEQPPIAD
                     YDELDRRMRNGELTLAIEIPPGFGRDLLRGRQVAIGAWFDGAMPQLAETVRGYIQGMH
                     QHWLADQARGMQGGHTTGRISVENRFRYNPDVKSLPAMVPAVIPLLLMMLPAMLTALA
                     VVREKEMGSIINLYVTPVTRTEFLLGKQLPYVALAMVNFALMTLLAVTVFEVPIKGSV
                     LALVLASVLFCLISTGMGLLASAVTRSQIAAMFFAMVGTLIPTTQFGGLMDPVSSLEG
                     GARVIGEIYPASHMFIISRGVFNKALGFGDLSANFWPLLLAVPVIVGTAIALLRKQEV
                     "
     gene            14417..15544
                     /locus_tag="PK242_01530"
     CDS             14417..15544
                     /locus_tag="PK242_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ92735.1"
                     /translation="MWQRLSNIYHLGVKELWSLWRDPIMLVLTVFSFTFAVYSAASSM
                     PETLNNAPIAIVDEDQSQLSQRIVSAFYPPQFTAPFLIDQSRVDPGMDSGVYTFALII
                     PPNFQRDVLAGRDAQVQLNTDATRMSQAFNGSGFIQQVVLGEVQEFVQRYRAGAEPAV
                     GLELRARFNPALEKSWFGGLAQIINQITMLSIVLAGAALIREREHGTVEHLLVMPVTP
                     AEIMLAKIWSMGLVVLLASAVSLHVVVRWALGVPVAGSLPLFFFGAALLLFATTSMGI
                     FMATVARNMPQFGMLVMLVLLPLQMLSGGQSPRESQPGWLQDLMLAAPTTQFVKFGQA
                     ILFRGAGIEVVWVQLLAMALIGAALFAFSLARFRKTIGQMA"
     gene            complement(15686..17647)
                     /gene="htpG"
                     /locus_tag="PK242_01535"
     CDS             complement(15686..17647)
                     /gene="htpG"
                     /locus_tag="PK242_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOZ92736.1"
                     /translation="MSTQTHAFQAEVANLLHLVTHSLYSNKEIFLRELISNASDACDK
                     LRFEALADSSLYGDQPELRVRVAFDRNARTLSISDTGVGMSEQETIEHLGTIAKSGTR
                     EFMSRLTGDQKQDAQLIGQFGVGFYSGFIVADRITVETRRAGLPPEQGVRWVSSGSGD
                     FSVERLTLPERGTRVILHLREDALEFADGWRLKELIGKYSDHIALPIQMEGEKWEDGK
                     EEGQPGQMVKTGDWETVNQATALWTRPRKDVTDEQYRDFYQQVAHDWQPPLAWSHNRV
                     EGSTEYTQLLYLPSHAPMDLWDRDKKPGVKLYVRRVFIMDDAEQLLPRYLRFVKGVVD
                     SADLPLNVSRELLQESRDVRSIREGNTRRVLTMLEELAKAPAEAPAEAPAEAAGGEPA
                     TDVQNPPPAGDRYATFWREFGAVLKEGLGEDHANRERIARLLRYASTASDTPAVSLAD
                     YKARMKDGQKAIYYIAADTLAAASHSPQLEIFRKKGIEVLLMTDRVDEWALSYLREFD
                     GTPLQSVAKGAVDLGELQNEAEKQAAEAAAEAVRPLLDKLKDVLKDSVEDVRVTTRLV
                     DSPACLVVKDSGISLQLARVLKQAGQPVPDTRPVLEINPEHALVKKLDGHARFDDLAQ
                     VLFDQALLAEGGLPDDPAGYVRRVNALLV"
     gene            complement(17940..19238)
                     /locus_tag="PK242_01540"
     CDS             complement(17940..19238)
                     /locus_tag="PK242_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ92737.1"
                     /translation="MTPTPPPGPDRPKQAARKRWRAADLRGAARLATQATMGVTRIVE
                     GVHRSVLGTLGLPGAAAPGRTRGITGMVYRSIQGVTLGVDAALQAALRRLEPWLERAQ
                     GAPDESAEAERDAVLAALNGVLGDRLAADDNPLALPMALRQHGRALELAALHAQGAAT
                     GRVLLLIHGLCMNDRQWLRSGHDHGAHLARTLGYTPVYLRYNTGLHISVNGRALAQHL
                     EALLASWPVPVQELGILAHSMGGLVARSAVWHATQTGQRWPAQLRHLIFLGTPHHGAP
                     LARAGPWVDLLLASTPYSRPFTRLGHLRSAGISDLRYGHVLDADWQGRDRLARSPDTR
                     TPVPLPDGVACHAVAATLAAQRSPLAERLVGDGLVPLRSALGAHDNPARCLAFAPADQ
                     YVVAQRGHLDLLSDAAVARQLVRWLTPPGERDPATATAER"
     gene            complement(19265..20011)
                     /locus_tag="PK242_01545"
                     /pseudo
     CDS             complement(19265..20011)
                     /locus_tag="PK242_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            complement(20157..20864)
                     /locus_tag="PK242_01550"
     CDS             complement(20157..20864)
                     /locus_tag="PK242_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ92738.1"
                     /translation="MAHPSALHERRRPLNAAELAGVPWLSHLKAEEFDRAAPELRISL
                     AEPGELVCRRGRPVTYWFGVVDGLLKMSTETAEGQTITYSGLAPGGWFGEGTVIKREP
                     YRYNVSALRRSLVAGLPADTFHWLLDHSIGFNRFIMNQLNERLGHFIGTLEADRTPKP
                     EQRVARSIAALVNPVLFPGVGEVLRITQQELAYLVGLSRQRVNEALQSLSAQQLLRIE
                     YGGLRILDLAALRSRDF"
     gene            21008..22957
                     /locus_tag="PK242_01555"
     CDS             21008..22957
                     /locus_tag="PK242_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ92739.1"
                     /translation="MATTFPRLLQKHAAERPGDPALREKEYGIWQTWSWQRALDEVRW
                     LACGLTALGFTPGQNLAIVGDNRPHLYLMFLATQSLRGVPVPMYQDAVATEMAFVLRD
                     AEVHFALAEDQEQVDKLLELRAATGEQAVPGLTHIIYDDPRGMRNYRQPGLISVDELM
                     ALGRAHEREHPQLFADLVAQGQPDDVAVILYTSGTTGRPKGVCQTHAAFVASARGGVE
                     TDGLKAGDDVLSYLPPAWVGDFLFSMAQWLVAGFTINCPESSDTVGIDMREIGPTYYF
                     APPRVFEGMLTSVSIRMEDAARFKQRLHAHFMGVARRVGSDLLDGKPVGLADRLQYAI
                     GNLLVYGPLKNAMGLSRIRVAYTAGAAIGPDLFRFFRSIGVNLKQLYGQTETCAYVCI
                     QRNGQVKLESVGQAAPGIELKIADSGEVLVKGVSVLKEYYKRPDATAEVIDENGYFRT
                     GDAGVLDRDGHLRIIDRAKDVGKLSGGAMFAPNYIENKLKFFPQIKEAVCFGHEREQV
                     CAFINIDFEAVGNWAERQGLPYAGYVDLAGKPEVLAMMKDCVEQVNAGLAAEEGMQDT
                     QIARFLVLHKELDPDDDELTRTRKVRRGFIAEKYGVLIDALYSGRAEQFIETAVKFED
                     GRAGKVSATLKILDARTYPPVRSAA"
     gene            22954..23814
                     /locus_tag="PK242_01560"
     CDS             22954..23814
                     /locus_tag="PK242_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ92740.1"
                     /translation="MNTMATETIAVSAHQTGAAAGSDLQSGGKKIGDVILDVQNISLR
                     FGGVKALTDISFNVREHEIRSIIGPNGAGKSSMLNCINGVYTPSEGSITFRGKTFSHM
                     NSRQVAEMGVARTFQNLALFKGMSVIDNIMTGRNLKIKSNLLMQALRIGPAEREEIAH
                     REFVEHIIDFLEIQAHRKTPVGQLPYGLQKRVDLGRALAMEPQVLLLDEPMAGMNVEE
                     KQDMSRFILDVNDEFGTTIVLIEHDMGVVMDISDRVVVLDYGKKIGDGTPDEVRGNED
                     VIRAYLGTSH"
     gene            23835..24764
                     /locus_tag="PK242_01565"
     CDS             23835..24764
                     /locus_tag="PK242_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ92741.1"
                     /translation="MGFFLETLFGGLMTGMLYSLVALGFVLIFKASGVFNFAQGAMVL
                     FAALAMARLAEWLPQWLGFENRVLANLLAFALAALIMWAVAWLIERLVLRHLVNQEGA
                     TLLMATLGITYFLDGLGQTLFGSASYQINIGMPKDPMFLFENVFQGGVLVNKEDLVAA
                     LIAAALVTGLTLFFQKTKTGRALRAVADDHQAAQSIGIPLSRIWVIVWTVAGVVALVA
                     GMIWGSKLGVQFSLATVALRALPVVILGGLTSVPGAIVGGLIIGVGEKLSEVYLGPFV
                     GGGIEIWFAYVLALVFLLFRPQGLFGEKIIDRV"
     gene            24833..25909
                     /locus_tag="PK242_01570"
     CDS             24833..25909
                     /locus_tag="PK242_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ92742.1"
                     /translation="MFYRENGQFKTSYRADQQIFPIAQDRWAVLLLVACAFVVVPLLA
                     SEYLFLSVLIPFLIMAMAAIGVNILVGYCGQISLGSGAFMAVGAYAAYNMYARIPGMQ
                     MLVAVLTGGAFAALVGIVFGIPSLRVRGLYLAVATLAAQFVCDWAFLRISWFTNHSTS
                     GSVSVSNLHVLGLPVETPAQKYLFCLSLLVVFGLLAKNLVRSAVGREWMAIRDMDVAA
                     AVIGIRPMYAKLSAFAVSSFIVGVAGALWGFVNLGSWEPAAFSVDRSFQLLFMVIIGG
                     LGSILGSILGAAFIIVLPIVLNLLLPWLGSLFGLEVSIAAVEHTQVMLFGALIVWFLI
                     VEPHGLAKLWSVGKQKLRIWPFPH"
     gene            25966..27288
                     /locus_tag="PK242_01575"
     CDS             25966..27288
                     /locus_tag="PK242_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ92743.1"
                     /translation="MKFRQLTLAAAAALTLGASASAMAQAQQFFPVLSGRTGPVAPNA
                     TPWANGHNDYMKLVNARGGINGVMTLVEECETAYATDKGVECYERLKGKHGGATVFQP
                     LSTGITFALTEKVPGDKISLITSGYGRSDTADGGVFKWNFPLIGHYWVAGDTVLQHIA
                     KKEGGWDKLKGKKFAIVYHDSPFGKELLPIVQERAKMHGFELQLLPVPAPGVEQKAIW
                     LQVRQQRPDFVIMQTWGVMTPTAIKEAVATGYPREKMFGTWWSGAEPDLKDVGAAAKG
                     YNAVMMQHGAEPQSAVVKEILEKVHAKGQGTGPKEEVGSVLYMRGVMGAMLAVEGVRA
                     AQERFGKGKVMTGEQARWGYENLNLTQAKLDALGFKGVMRPVSTSCQDHIGSGWARVH
                     TWDGSKFVWSSDWLEADQQIIKPMVKQSADKYAAEKKLTRRTAADCGS"
     gene            27413..28228
                     /locus_tag="PK242_01580"
     CDS             27413..28228
                     /locus_tag="PK242_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ92744.1"
                     /translation="MTTASAPIVLTVNGIEVIYNHVILVLKGVSLTVPESGIVAILGG
                     NGAGKTTTLRAVSNLLKGERGEVTKGSIELRGERIENLSPAELVKRGVVQVMEGRHCF
                     AHLTIEENLLTGAYTRSDKGEIAANLDKVYTYFPRLKTRRTSQAAYTSGGEQQMCAIG
                     RALMCNPSMVLLDEPSMGLAPQIVEEVFNIVRDLNTKERTTFLLAEQNTNMALKYADY
                     GYIMESGRIVMDGKASDLASNEDVKEFYLGVGGGERKSFKDVKSYKRRKRWLA"
     gene            28333..29583
                     /locus_tag="PK242_01585"
     CDS             28333..29583
                     /locus_tag="PK242_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ92745.1"
                     /translation="MTEYFDALETRDPAAREAALLAALPRQLAHAQQHSAAFADILAG
                     VDAAAVTSRAALARLPVTRKSELLARQQASRAAGRDPFGGFATGRWGSAMRRVYASPG
                     PIYEPEGCASDYWRTARALYAAGFRAGDLVHNAFSYHMTPGAFILEAGALAVGCTVFP
                     AGTGQTEQQLQAIAELRPQAYVGTPSFLRILFEKAAEGGADIRSLTKAMVSAEPFPPS
                     LRDWFAERGMTAYQSYATADVGLIAYETAARQGLVLDEGVIVEIVRPGTGDPVPEGEV
                     GEVVVTVLNPDYPLIRFGTGDLSAVLAGPCPTGRTNTRIKGWLGRADQTTKIRGMFVH
                     PGQVAEIVRRFPGVGRARLVVSGAMANDQMTLRVEAADLPGLAERIADAVRDVTKLRG
                     QIELVAPGSLPNDGKVIEDARSYE"
     gene            29706..30695
                     /locus_tag="PK242_01590"
     CDS             29706..30695
                     /locus_tag="PK242_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOZ92746.1"
                     /translation="MSPLFKLSIATVAALAALGAQAQAFPAAGKTVTLIVPFAAGGPT
                     DKVARDLAEALRKPLGGATVVIDNAAGAGSTIGAAKAFRAAPDGYTLLVTHIGMATTP
                     ALYRKLPYNVEEFEYLGLINEVPMVVVSKPGLPANNWAELKAWIGSNKGKVNLANAGL
                     GAASHLCGLMLQQALATEMTTIAYKGTAPAMTDLMGGQVDIMCDQTTNTTSQIEGKKI
                     KAFAVTTPKRITMPPVYKDLPTLEEVGMKGFNVSIWHGLYAPKGTPAAVVKVLNDALQ
                     AALKDADFIKKEEALGAVVAHDDRVTPAGHKKFVTAEVAKWGAVIKAAGQYAD"
     gene            30757..31134
                     /locus_tag="PK242_01595"
     CDS             30757..31134
                     /locus_tag="PK242_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92747.1"
                     /translation="MNRPLRLVDSITELRPGADTGCLAVSGSHGGISSAHYALAAQPM
                     LAVFNDAGVGKDAAGLAALPFLQRHGIAACTVAHTSARIGEARSTLHDGVVSHCNAQA
                     LSLGIEPGQACAALIRQLQGSGG"
     gene            31215..32153
                     /locus_tag="PK242_01600"
     CDS             31215..32153
                     /locus_tag="PK242_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ92748.1"
                     /translation="MAWAQGAWPSKPVRIVVPFAPGGTTDILARALAPELSKAFGQQF
                     IVDNRAGAGGNVGAEVVAKSPGDGYTLLMGTVGTHGINRALYPRLPYDPIKDFAPITL
                     VASVPNVMEMNADKAAQLGIHNVQDFIRYVKAHPGRLNMASSGNGTSIHLAGELFKHM
                     TGVFMTHIPYRGSGPALLDMVAGNMDVMFDNLPSSMAQIKAGKLKPLAVTSARRSAAL
                     PDVPTVEEAGGPALKGYEASSWFGLLAPAGTPADVVSRLQQESAKALASPAVKEKLLA
                     QGAVPGGIPPGEFALMIADEHAKWAKVVKASGAKVD"
     gene            complement(32214..32558)
                     /locus_tag="PK242_01605"
     CDS             complement(32214..32558)
                     /locus_tag="PK242_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HOZ92749.1"
                     /translation="MLYKFKSQAAADVIMLKASAEELLKIIGKPASPTGIVTVDQAPA
                     AIAALKAEIERREAQGQGHAAPTSDDEARTQATADEFARQELVTLRQRAAPFIELLQI
                     SSAAGKDVVWGV"
     gene            32723..35053
                     /locus_tag="PK242_01610"
     CDS             32723..35053
                     /locus_tag="PK242_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HOZ92750.1"
                     /translation="MKIVCIGGGPAGLYFALLMKRLNPAHDITVIERNRPYDTFGWGV
                     VFSDATMDNMRQWDAPTADAIQQAFNHWDDIELLFKGQVIRSGGHGFVGIGRKKLLNI
                     LQARCEALGVRLVFEQEVDDDAAFADADLIVASDGINSRIRTLHQAQFRPELVVRPNR
                     YIWLGTRKLYDAFTFIFEKTEHGWFQAHIYKFDQHTSTFIVECPEPVWRAHGLDQATQ
                     EASVAFCEKLFARHLQGERLMTNARHLRGSAWLNFTRVKCDRWWLRNANGAHVVLMGD
                     AVHTAHFAIGSGTKLALEDAIGLTREFKRLGDSPAQIEQVLSDYQASRSVEALRLQNA
                     AWNAMEWFEVCGERYCDQLEPPQFMYSMLTRSQRISHENLRLRDRQWLEGYERWFARR
                     DGLAVADAARPPPPMFTPYTVRGVTLKNRVVVSPMAQYSCVDGVPGDYHLVHLGARAL
                     GGAGLVMAEMTCVSPEGRITPGCPGLYNAEQKAAWQRIADWIHEHSDARFGMQIGHAG
                     PKASTRLAWDGTDLPLPQGNWPIVSASPQQYLPGISQRARALTQAEMQVILQQFVQAA
                     QSAADIGVDWLELHCAHGYLLSAFISPLTNQRTDEYGGSLENRLRFPLEVFRAMRAVW
                     PPQRPMSVRISATDWVEGGTTPADAVAIARAFKEAGADLIDCSSGQVSKQEQPVFGRM
                     WQTPFADRIRQEAGIATMAVGAISEADHVNSIIAAGRADLCAVARPHLANPAWTLTEA
                     ARIGYADMTWPRPYFQGKRQLEAGFERARAAGGAPI"
     gene            35064..35528
                     /locus_tag="PK242_01615"
     CDS             35064..35528
                     /locus_tag="PK242_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOZ92751.1"
                     /translation="MDLEARAHAEHPEALRLWLRLLTCTQMVERDLRNRLREAFDTTL
                     PRFDLMAQLERVPEGLRMNELSRRLMVTGGNVTGITDQLEAEGLVERVPVEGDRRAFH
                     VRLTPAGRQQFQAMAQAHEQWIVDAFAGLTSAEIRTLHQLLGRVKQHFLETT"
     gene            35525..36382
                     /locus_tag="PK242_01620"
     CDS             35525..36382
                     /locus_tag="PK242_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HOZ92752.1"
                     /translation="MKHYIPSANPMRPQYRAQAAYQARHFAWQCDADGVATVTLDRPE
                     RKNPLTFDSYGELRDLFNGLRHATDVKVVVVTGAGGNFCSGGDVHDIIGPLTKMSMPE
                     LLEFTRMTGDLVKAMRGCPQPVIGAIDGVCAGAGAMMALACDLRYGTAATKTAFLFTR
                     VGLAGADMGACALLPRVIGQGRASELLFTGRAMNADEGLAWGFFNALHGSGELLVKAQ
                     EMARQLAAGPMFGHAMTKNMLQQEWAMTIDQAIEAEAQAQAICMQTQDFRRAYEAFAA
                     RQKPVFGGD"
     gene            36382..>36830
                     /locus_tag="PK242_01625"
     CDS             36382..>36830
                     /locus_tag="PK242_01625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ92753.1"
                     /translation="MSTGILQPTALRPSTSHLGLPFFDEAHRRIAAELPDWTNAQGVD
                     ESDDRAACRDWVRRLGAGGWLRYCVPGDCGGALPQLDSRALVLLRETLAFYSPLADFA
                     FAMQGLGSGAISIGGSASQRQAYLPQVARGARIAAFALSEPEAGSDVA"
BASE COUNT         5668 a        12411 c        12838 g         5913 t
ORIGIN      
        1 ggcatggcca accacttcta ccgcgagcgc gtggcccagc acctgctgat cggcattcgc
       61 gccatcgaac gcctgcgcga aaacggcctg caccgtctgc acagccgcaa gctgcgcagc
      121 tttgccgagg tggcgttcca gtgatccggc gccgctgacc cgcggctttc agcgcgcgcc
      181 gatcacggcc gccggtcgtc ggcgtgcgcc tgggcgtcgt gcgcgtgctc gtcctgcagc
      241 tgcatggcgg cggccagcag gtcgctgccg gagccggtcc attccggcgg gacctgtgag
      301 ggcgagacgg cgctgctgtg gaaggtcctg gcctgctccg gcaccaggtg gctcaccggc
      361 gcgggtgtgg gcagccgccg gcgcgtcagc tcttcggcga tctgtaccgc cgaggccggg
      421 cggccggcga tggtcggccg gatgccgggg cttgccgcca gctcttcggc cgtgggcgga
      481 tgcagcgaga agctgtgcgc gtcggccagc ggccagtaca gccgatagac gttgtgctgg
      541 tgctccaggt cgttcaggat ggcaccgaag cgcatggtcg gcatcatggc cgacagcagc
      601 cgcacgcggg tggcgtcgat gcggcgcacg atgtggtggg tgtagagctg gtcggggtgc
      661 atcaggccgg ccgcctgcag cagctccttg agcgcgtgca gggtgttggc gtggtactgg
      721 gccacgcgcg tggccttgtc ggccaccacc agcgcgcgct cgcgcagcgg gtcctgggtg
      781 gtgatgccgg tggggcaaaa ccctgtgtgg cacacctgcg cctggatgca gcccagcgcc
      841 atcatgaagc cgcgccccga attgcaccag tcggcgccca gcgcgaacat gcgcgccagg
      901 tcgaacgagt tgatcacctt gccggcggcg ccgatcttga tgcgatggcg caggttgacg
      961 ccgatcagcg tgttgctgac caggttcaac ccctcctgca ccggcgcgcc catgtggtcg
     1021 gcaaactcga tcggcgccgc gccggtgccg ccttcggcgc catcgaccac gatgtagtcg
     1081 ggcgtgatgt cggtctccag catggccttg acgatggcga accattccca gatgtggccg
     1141 atgcacagct tgaaaccgac cggcttgccg cccgacagct ggcgcaggcg ggcgatgaag
     1201 tgcatcagtt cgatcggcgt cgagaaggcg ctgtgcgccg acggcgagac gcagtcctga
     1261 ccgaccggga tgccgcgtgc ggcggcgatt tcgggcgtga ccttggcgcc cggcagcatg
     1321 ccgccgtgcc cgggcttggc gccctggctg atcttgattt cgatcatctt gatctgcgga
     1381 tcgacggcct gtctggcaaa gcgctcgggg ctgaagctgc cgtcgtcgtt gcggcagccg
     1441 aagtagccgg agccgatctc ccagatcagg tcgccgccgt gctggcggtg gtagcggctg
     1501 atgccgccct cgccggtgtc gtgcgcgaag ccatccatcc tggcgcccag gttgagcgcc
     1561 aggatggcgt tggccgacag cgcgccgaaa ctcatggccg agatgttgaa tacgctggcg
     1621 ttgtacggct gcgtgcaggg cgccacgcct tcgcgcggca cgccgggctg accgccgatc
     1681 cagatgcgga agtcgtgcga ttcgatatgg gtggtctgca tggagtggtt gatccactcg
     1741 tagccgttgg cgccgacgtc cagcttggtg ccgaaggggc gcacgtccgg cacccccttg
     1801 gcgcgctgat acaccacgct gcgctgcgcg cgcgtgaacg gttcgccggc ctcgacgtcg
     1861 ccttcgatga agtactgacg gatttccggc cggatgaatt cgagcgcgaa gcgcaggtgc
     1921 ccgaggatcg ggtagttgcg caagatggcg tggcgcgtct gctgcaggtc atagacaccc
     1981 acgcccacca ccacggcgca cagcagcatc agtgccgtcg accatggacc ggggccggcc
     2041 aaggccatcc acaccagcgc gagcagggtg cccagcgcgc acagggccag cgtgctgaag
     2101 cggatcgact ggtccagaaa ggcccgaacg tgcatggcag agctccggtg ttggccgaga
     2161 gcgctcctgc cgatccgcca gccgccacag cgggctgagc acagggcagg cgccctcgga
     2221 tcacatctta gccatgcccc gcccggtgga aaaccccggg cgcgggcgct gcgccggccg
     2281 cgctggcagg ggcggccggg cgccggctca gtaccggggc tgcaggcgcc gcagcgtggc
     2341 cgccacctcg tcggtgatgc ggaacccgtc gccatggcgg gccgccagcg cggcgcagcg
     2401 ctgctcgaag gcctcgatgc cctgaccgta gatgaactgc atggcgccgc cggtccaggc
     2461 gggaaagccg atgccgaaga tcgagccgat gttggcgtcg tgcacgctgg tcagcacgcc
     2521 ttcggccagg cagcgcgcgg tctcgaccga ctggcgatag agcaggcggt cctggatgtc
     2581 ctgcacgctc caggccgtgt cgggtttctc gaacagcgcc ttcagctggg gccataggtg
     2641 cttcctgccg ccctgcgggt agtcgtagaa gccgccaccg ccggcccggc ccgggcgccc
     2701 cagctccttg accatgcgct cgaccagcag ctcgccgggc gtggcctggt aggtcttgcc
     2761 ttcggccgcg aagtcggcac gggtctgatc cagcacatgc accgacagcg acagcgcggt
     2821 ttcgtccagc accgccagcg gccccaccgg cagaccggcc tgcacggcgg cgttctcgat
     2881 ggcggcggcg gggatgccct cgcccagcat ggccgcgcct tccatcacga aggtgccgaa
     2941 ggtgcggctg gtgtagaagc cgcgcgagtc gttcaccacg atgggcagct tgcccagcgc
     3001 cagcacgtag tcgtaggcgc gcgccacggt ttcgtcgtcg gtctggcggc cgcggatgat
     3061 ttccaccagc ttcatcttgt ccaccgggct gaagaagtgg atgccgacga acttgcgcgc
     3121 atcgcggctg gccctggcca ggccgctgat cggcagggtg ctggtgttgc tggcaaaaaa
     3181 gccgccctcg gccagcatgg gttcggcctc ctgcgtgacc ttggccttca gctcgcgttg
     3241 ctcgaacacg gcttcgatga tcagctcgca gcccttcagg tcggccgcgt cggcggtggg
     3301 cgtgatgcgc gccagcagcg cctgctgctc gggcgcgctc atgcggccct tgtcgacgcg
     3361 cttttgcgtc agtttggcgc tgtaggcctt gccctgctcg gctttgtcgg gcgacacgtc
     3421 tttcagcacg gtggcgatgc cgcggctggc ctgggcccag gcgatgccgg cgcccatcat
     3481 gccggcaccc agaatgccca ccttctgcgg cttgaagcgc gtggccacct gcgggcgcga
     3541 ctggccgctc ttgatggcgt tcaggttgaa gaaaaaggtg ttgatcatat tgcgcgccac
     3601 cgggccggtc atcaggctgg ccaggtagcg gctttcgatg cgcgaggcgg tgtcgtagtc
     3661 cacctgcgcg ccttcgacca tggcggccag cgcggcctcg ggcgcggggt agcgcccacg
     3721 cgtcttgagc ttgagcatgg ctggcgccac caccagcgca ccggcgatct tggggttggc
     3781 gggggtgccg ccaggcatct tgtagccctt gacgtcccag ggatgcgccg cctgcgggtg
     3841 ctcggcgatc caggccaggg cgcgcgggcg cagctcatcg ctgctggcca ccagctcgtg
     3901 caccacgccg atctccagcg cctcgcgcgg gccgaacagc ttgctctcca tgatgtaggg
     3961 ctgcgcgccc atcaggccca gcaggcgggt gaatttggtg atgccggtgc cgccgggaat
     4021 caggcccagc gtcagctcgg gcaggcccag ctgcagtttg gggtcgtcca ccgcgatgcg
     4081 gtggtggccg accagcgccg tctccatgcc gccgcccagt gcgctgccgt tgatgcaggt
     4141 gaccaccggg atgcccaggg tctccagtga gcggaagcac ttcttgatgc gttcgatctc
     4201 ggtgaacacg ctggtcgcgt ccgcttgcgt ggtgcgcatc acgcccttga ggtcggcgcc
     4261 ggcgaaaaag gttttcttgg ccgaggcgag gatcaggccc cgcaggcgcg ccttgtcgtg
     4321 cagcacctgg tcggtcaccg ccaccaggtc gtcctgccat tgccggcaca tggtgttgac
     4381 gggcgagccc tgctcgtcaa aggtgatggt ggcgacgggg ccgctggccg tgtcttcgat
     4441 cgcgtagcgc agtgtctgga gttccatgat gactatgaaa ttgatagctg ccggcgcttg
     4501 accatcaagc gccggaggcc gatgagattc tgatgttcag gggcggcacc gcagcgcgcg
     4561 gatcacagcc gctcgacgat ggtggcgatg cccatgccgc cacccacgca cagcgtggcc
     4621 aggccgtagc gcaggccgcg ccggtgcagc tcgtcgatca gcgtgcccag gatcatggcg
     4681 ccggtggcgc ccagcgggtg gcccatggcg atggcgccgc cgttgacgtt gaccttgtcg
     4741 tgcggcacgc cgaacgcctt catgaacttc atgggcacgg cggcaaaggc ctcgttcact
     4801 tcgaacaggt cgatctgatc gatcgtcagg cccgccttgg ccagcacctt gcgtgtggcc
     4861 ggcatcgggc cggtcagcat gatggtcggg tctgcgcccg agacggcggt ggcgacgatg
     4921 cgtgcgcgcg gggtcaggcc atgggtgcgc gccgccgctt cactgccgat cagcaccgct
     4981 gccgccccat cgacgatgcc cgaagagttg cccgcgtggt gcacatggtc gatgcgctcg
     5041 acctggggat agcggccgat ggccacggcg tcgaaaccca tcgcccccag ctgctcgaac
     5101 gaaggtttga ggccggccag cgcctccagc gtggtgcgcg gcttgatgaa ttcgtcctcg
     5161 gacaagatgg tttgtcccag aaagtccttc accggcacca cggcgccggc aaagcagccg
     5221 ctcgcgcgcg cccgcgctgc gcgctgctgc gactcgacgg cataggcgtc cacgtcctgg
     5281 cgtgtgaagc cttcgagtgt ggcaatcagg tcggccccga tgccttgcgg cacgaagtcg
     5341 gtggcactgt tggtggccgg gtcctgtgcc caggcgccgc cgtcggagcc gatgggcacc
     5401 cggctcatgc tttccacgcc gccagccacc accaggtctt cccagccgct cctgaccttc
     5461 tgtgccgcca ggttgaccgc ctccaggccc gaggcgcaga agcggttgag ctgcacgccc
     5521 gcgcattgcc agtcccagcc ggccttcagc gccgccacct tgggcagcac cgaaccctgc
     5581 tcaccgatcg gcgagacgat gcccatcacc acgtcgtcga ccgcggcggt gtcgaaatcg
     5641 tggcgcgtgc gcagcgcctc cagcgtgcca gccagcagat cgatcggctt gacctcgtgc
     5701 aggctgccgt ccttcttgcc cttgccgcgc ggcgtgcgca cggcgtcgaa tacgtaggct
     5761 tcggtcatgg gaagtctttc gtgcgtaagt gaagtggggg gagcgtcagt atagatattt
     5821 tccaaagcga tcgcttgata aaaatagatc gggcattccc gcagcggctg gcgccgatcc
     5881 gaaggtgggt acgggccgtt cgcacccgct caggcggcgc gggtgcgcag atcgatccac
     5941 tggcgctgct cgcgcagggc cgcggccgag cggtgaaaca gcgggtccag ggcgcccaga
     6001 tcgtgcgaca gcaggtcggc gcccgcctgg cgcggccccg ccggggcgct ggccggcggc
     6061 cgcgccgccg ccacgctggg tgcgacgcgc cggaaggtgg cggtgacggt gcgcgcgtcg
     6121 tcgtcgaagt cgccgtggtg catggcaccc gaggccgaca gcgaatcggc cggctcggtg
     6181 ttgggggcca gcaccaggtc cacgcccagg cgctgcatca gcgcgcggtc gatgtggcgc
     6241 tccatgccga ggaacggttc gccgtcgcga aacaggggga tccgcggccg ctcctcgaag
     6301 gcgtgggaga tcaggtacag cagcgacttg tggtagatgc cggcgcagtg gtcgtcctgc
     6361 tcgaccttgt cggtgagggc gaacagggcc atcttttcga tctggcgaga ctccagcgcg
     6421 ggctggtagt gccgggtgaa cagctcgacc cggcaggccg gcgcccacag ggtgcaggtt
     6481 ttcaccacca ggccgcgcgc gcccagcaga tcgaccagcg gcgccagcag aaccgagccg
     6541 gcgctgtggc cgaccaggtg cacttccagc ccgggcaggc gggcacgcag cgccagcagc
     6601 agatcggcca cgcgctcggc cgcgccgccc cgggcgctgg cggccagggc gttctgcttc
     6661 atctcgtccc aggccgattt gccggtgagc cggcgcgcca gcggctccag ggcatcgtcg
     6721 agccggtcga gcatgaagtc cttggtggcg tcgagcaacc cttcgggccg gcgccggctg
     6781 accgcgtcct tgatgatgtt ggtgatggtg gtccagtagt cggtgcgcca gatgaaggcc
     6841 agcgggtaca cctgggcgtc cagcagcagc gggcggtatt cggccagccg ctgcaccccg
     6901 gcctgctcgc tcaccaggcc gccatgggca tacagcagca cgcgcggcac cgcccagccg
     6961 gcgctgacgc gcgggatgtc ttgctcgaac acgcgctgca gctcctcggg cgtgctgccg
     7021 tagtcaccgc cggccttgag cgtgccgttg ttgccgacgc tgacgatgtg aggtcgcagc
     7081 tcggcgaacg agtaacccac cgactgcgcg gcactgcggg catggatgga ggcggtcgac
     7141 gcgccgcgct gcagcgtgat gggggcaccc agacgggcca cccagacgtc ggtgccgtgc
     7201 tccagccagt cgtcgtagct cagcaggcca aagccgttgc ggccccagcc cgggccccag
     7261 gaattctgga cccagaagcc gcgctcgtcg taggccacga tggcaaaggc gtgaccgccc
     7321 aggatgcgtg tcgaagggcc gatcacgccg tcggcgccga ccgccgacca gccggcgtgc
     7381 accgtgcagc tggcatagag cacgccgacc tcggcgatcg ccgagtgcat ggccaccagg
     7441 tccttgtggt tgacgcggaa gtaggccccc agcgggcggc gcagcgcgtc gctgacgcgc
     7501 gcctcgtcca ggccgccggc gggcggcacc gggcgggtgc gtcggccgcc gcgcgggcgg
     7561 ctggggtaga gcgtttcggc gcacacgccg tgcttgtacc agcccttcat ggcgccgcgc
     7621 gcgctggagc cgtcgtagtc ctcgcccggc cattcgtcgt agcggcgtgc cagttcgtac
     7681 agcatgcgcg ggctgaccgg cgtggcgtcg gccaccacac gccggcgccg cagcaggtag
     7741 tgcgccaccg tggccaggcc gtagccggtg caggcgcctt cggtgccctg gtcgagcacc
     7801 ggcacctgcc attcgaggta ctcgcccagc gccacgtggg agggcacctc gaccagcgtg
     7861 ggcatgaaca tctggtcgcg aaagtccagc gtgtccggtc gcgcgtccag gcggcgcgag
     7921 ggcatcgcgc cggtcgccgg gcggcggccg cggggcgtgc gggtggctgc gagggcgtct
     7981 ggccgctcgg cctggtcggt caccatcgct tcgtgtccgc gcgccagccg cagcgccgcc
     8041 gagccggcgc gggtgcgcgc gcggcgccgg gccgctgcgg cggccaccgg cagcgtggag
     8101 gcgctgctgc cgaccggctc gggcagaccg ccttgcagca cctgcagggc cagcgcgtgc
     8161 aaccgctggc ggtcggccag gccgttcagc ccgccgttga tgcgccgtgt gatggtctcg
     8221 aagtcgcccg cgtcggccag ggcattgagg ccgttgcggt cccagaacag ggcggcgatc
     8281 tcgaagccga cctcagggtc ggcggcctgc tcgggctggc ccaccagatc cagcccgagc
     8341 agctcgccgt agcgtgcgta attggcgcgt ccggtgatct ggatcggccc gcgccccttg
     8401 aagcgccggc cgtcgccgcg ctgggtgttg ccgaggcggc gcgccagctc gccgggcggt
     8461 tcgtagcgct tttgcgccgg ggtggggccc cacagctcct ccaggaactg caactcgccc
     8521 gattcatgcc ccaactgcgc caggaaggcg gcgatccgct gcaggttggc gtcgatccgg
     8581 tgcgcctgca tggcgcggtt gagcggatcg aggcattgcg cccggcggcg cgtggatgcg
     8641 tggcgcatga tctgcgcgag ctgcaggtcg gtcagcatgg cggtctcctc ggtgcgcgcc
     8701 agccggcctt ggtgccggga ttgcgccaac aagccctcca gcgcatcgtc cggccatgat
     8761 agagcgcgca tggcggcgtg accagcggcg ccgtgcgtcg tctgccgtcg gcgtctgggg
     8821 ctgcggcgcg ttcaaaggca tgggccagat cacggcccat tccgtggtgg tgcaccgacg
     8881 ctgctgcgac agggtaggag aaggcaggcc atgccaggta cctggcgggc cacccaggcg
     8941 gatccgggca ggacaacgcc atgaggagga acaggcatca ggcccagcgt ccggacggca
     9001 tcgcgtcagc gaaggcgatc cacttcgcat tcgatgccga cggatgccca tcttggaaga
     9061 cgggcactac catcgacgat ctgttcctgg atcgatggcc catgcgatag gctccagagg
     9121 tatgtacaag cactgctttc gaatggcctg ctgtgcggcg gccctgctga ctgcagggtg
     9181 cgcctcgatg gcgccaccgc atgagcgacc catcgtcgcc atgcccccca ccttcgcgtc
     9241 tgcaacgccc gctgatgtgg gcacgcaaac cgtcgccgcc ctcggctggc gcagctattt
     9301 caccgatccc gcgctcacgc gactgatcga gatggccctg gcccataacc ccgacctgcg
     9361 gactgccgtg ctgcgcgtgc aggaagcgcg ggccacctac ggcgtgcagc gcagcgcgca
     9421 gctgccgacg gtgggtgtgg gcctggacgc gagccgcacg cgcacaccgg gcgacctgag
     9481 cccgagtggc agtgcggtga cggccaacca gttccaggcc agcgtgggca tcagcagttg
     9541 ggagatcgat ttctggggcc gcatcgccag cctcaacgag gcggcgctgc agagctatct
     9601 ggcgaccgat gcggcgcgcc gggcggcaag cgtgtcgctg atcgcccagg tggccgacgg
     9661 ctggctgggc ttgcgtgaag cgcaggaacg actgcgcttg gcacaggcca gcgcggccaa
     9721 ccagcgcgag tcgctgcgca tcttcacacg gcgcctggag gtgggggcgg cttcgtatct
     9781 ggagttgacc caggtgcaga cgctgctggt tcaggccgag ttgctggtgg cgcaaatgca
     9841 gcagcagcgc gacgcgcggc ggctgtccct gaacacgttg ctgggcgatc tgtcggacgg
     9901 cagcactgtg gcgaccgaag ccaacacgcc gctggacgac accatgatcg cacctgtggc
     9961 gcctggtctg ccatcggacc tgctgacccg ccggcccgac gtgctggccg cggagcatcg
    10021 gttgcagggc gccaacgcca acatcggcgc ggcgcgtgct gcgttttttc cgcagatcag
    10081 cctgacggct ttcggtggaa cggccagcac cgagctggaa cggcttttcc ggggcgccaa
    10141 ccgcgtctgg ggcttttctc ccagcctgtc cctgcccatc ttcgacgccg ggcgcaagcg
    10201 cgccaacctg gcattggccg aggtgcgcaa gaacctggcc gtggtgcagt atgagcagac
    10261 cgtgcagaac gccttccgcg aggttgccga cgccctgtcg gcccagcatt gggccttgcg
    10321 ccaggtggag gtgcaccagc gtgccctggc gatcgaacgc gaacgtgccc gtctgtcgcg
    10381 attgagctac gaccacggcg cgtccagctt tctcgaggtg ctcgacgccg agcgcaacct
    10441 gatctccgcc gaacagcaac tggtgcagac gcgccgtgcg ttgttgtcca gccgtgtggc
    10501 gctttacgcc gcgctggggg gcggatcaca cgacgtggac gagacgccgc ccggtccgac
    10561 gtctgcctcc cccgccaact tcacccgtcg ctgatcgcca tgatctcctc catcaagaac
    10621 aagttgcttg ccgtcctcgc catcgctgcg ctcgctgcgg gtggatggta tgcgtggcag
    10681 gcatccaccg ccaaggtgga caagccgtcc tttgtcgccg gcaacggtcg catcgaagcc
    10741 accgaggtgg acatcgccac caagctggcg gggcgcgtga acgacatcct cgtcacggaa
    10801 ggggctttcg tgaaagccgg gcaggtgctg gcgaccatgc aggtgcagac cctggaggcg
    10861 cagttgcgcg aggcgcaggc acagcgcgca caggccgtga cctccgtggc agcagccgag
    10921 gcgcaactgg ccatgcggca cagcgacaaa gccgcgctgg ccgctcaggt gcatcaggcc
    10981 gcggccgaac gcaatgccgc gcagcgccgc ttggcgcgct ccgaaaccct ggtgcgggag
    11041 ggggccacct cggtccaggc gctggacgac gatcgcaccc gcatggagag cgccgttgcc
    11101 gccgttgccg ccgcacaggc gaaggtgact tcggccgacg cgtccatagg cgccgccaaa
    11161 gcgcaggtgg tgagcgcgca atcgcaggtc agggcgctgg acgccacgct ggagcgcata
    11221 caggcggaga tcgctgacag tcagctcaag gcaccgcgcg atggccgcgt ccagttcgtc
    11281 gtggcgcagc ctggcgaggt gctgggtgcg ggcggccggg tgatgaacat gctcgatgtc
    11341 tccgacgtct acatgacatt cttcctgccc accgaggcgg cgggccgggt cgcgcttggt
    11401 gcggatgtgc gcctcgtgct ggatgccgcg ccggacttcg tgatcccggc caaggtttcc
    11461 ttcatcgcca gtgcggcgca gttcacgccc aagacggtgg agacggcgag cgagcgccag
    11521 aagctgatgt tccgggtgcg cgcccggatc gatcggcagt tgctgctcga gcacctcgaa
    11581 cacgtcaaga ccggcttgcc cggcgaggcc tgggttcgcc tcgatccggc gcaggactgg
    11641 ccgacccggc tggcgttgaa ggcaagacca tgacagggct ggccaacgca cctgtggtgc
    11701 gcgtggaagc gttgcgcctg cgccatggca aggtgcaggc gctggcgggc gtgagcctgg
    11761 agttgccggc cggccgcatg gtggggctga tcggccccga cggcgtgggc aagtccagcc
    11821 tgctgtcgct gctggccggc gcgcgcgcgg tgcaggaagg gcgcgtcgag gtgctgggcg
    11881 gcgacatggc cagcaagcgc caccgcgaca ttgtcggccc gcgcattgcc tacatgccac
    11941 aggggctggg caagaacctc taccccacgc tgtcggtgga ggaaaacctg caattcttcg
    12001 cgcgcctgtt cggccatgac agggccgagc gccgccggcg catcgacgag ctgacgcaag
    12061 ccaccggcct gcaccccttc ctcgatcgtc cggcgggcaa gctgtcgggc ggcatgaagc
    12121 agaagctggg gctgtgctgc gcgctgatcc acgacccgga tctgctgatc ctcgacgagc
    12181 cgaccaccgg tgtcgatccg ctggcgcgcg cgcagttctg ggacctgatc gcgaacatcc
    12241 gcaccggtcg ccccggcatg agcgtggtgg tcgccacggc ctacatggac gaggcccagg
    12301 gcttcgactg gctggtggcg atggacgacg gccgggtgct ggccaccggc acgccggccg
    12361 agctgctcga acgcaccggc agcgatgcgc tggaggccgc cttcattgcg ctgctgcccg
    12421 aaagcaggaa gcgcggccac gcgccggtgg tgatcccgcc gctgcaggcc gatgccgacg
    12481 acatcgcgat cgaagccaag gacctcacca tgcgcttcgg tgatttcgtc gcggtcgacc
    12541 gcgtgagctt tcgcatccgc cgcggcgaga tcttcggctt cctcggctcc aacggctgcg
    12601 gcaagtcgac cacgatgaag atgctgaccg gcctcctgcc ggccagcgcg ggtcaggcct
    12661 ggctgttcgg ccgcgaggtc gatcccaggg acatcgccac ccgcaaccgc gtcggctaca
    12721 tgtcgcaggc gttctcgctc tacagcgaac tgacggtgcg gcagaacctg gtgctgcacg
    12781 cgcggctctt ccacgtcgcc gagaccgaca tcccggcgcg tgtggccgag atggtcgaac
    12841 gcttcggtct tgggccggtg ctggacgagc tgcccgaatc gctgccgctg ggcatccgcc
    12901 agcggctgtc gctggcggtg gcgatggtgc acaagcccga gctgctgatc ctggacgaac
    12961 cgacctcggg cgtcgacccg gtggcgcgcg atgccttctg gcgcttgctg gtcgagctgt
    13021 cgcggcgcga ccgggtgacc atcttcatct ccacccactt catgaacgag gccgagcgtt
    13081 gcgaccgcat gtcgatgatg cacgcgggca aggtgctcga cagtgccgcc ccggccaagc
    13141 tggtggaaaa gcgcggcgcg gcgaccctgg agcaagcctt catcggctac ctggtggagg
    13201 ccggcggcgc aacgccggcc agcgcggccc cgatcggcgc cgtggcggct gctgcgccgt
    13261 cgcgggaggc ggcagcgcac gcgcgcagac ccttcagcct gcagcgcctg tacagctacc
    13321 tgtggcgtga agcgctggag ctgcagcgcg acccggtgcg cgccacgctg gcgctggccg
    13381 gctcgatcat cctgatgatc gtcatggggt ttggtattgg caacgacgtt gccggcatcc
    13441 gctacgctgt gctcgatcgc gaccagagcg cgctcagcaa cagctatctg ctcgacatcg
    13501 gtggttcgcg ctactttcag gagcagccgc ccatcgccga ctacgacgaa ctcgaccggc
    13561 gcatgcgcaa cggcgaactc acgctggcga tcgagatccc acccggtttc ggccgtgacc
    13621 tgctgcgcgg gcgccaggtt gccatcggcg cctggttcga cggcgcgatg ccgcaactgg
    13681 ccgagaccgt gcgtggctac atccagggca tgcatcagca ctggctggcc gaccaggcgc
    13741 gtggcatgca gggcggccac acgacgggcc gcatctcggt cgaaaaccgc tttcgctaca
    13801 accccgacgt caagagcctg ccggcgatgg tgccggcggt gatcccgctg ctgctgatga
    13861 tgctgcccgc catgctgacg gcgctggcgg tggtgcgcga gaaggagatg ggatcgatca
    13921 tcaacctgta cgtcactccg gtcacccgca ccgagttcct gctcggcaag caactgccct
    13981 acgtggctct ggccatggtc aactttgcgt tgatgactct gctggcggtg accgtgttcg
    14041 aggtaccgat caagggcagc gtgctcgcgc tggtgctggc ctcggtgttg ttctgcctga
    14101 tttccaccgg catgggcctg ctggcctcgg cggtgaccag gagccagatc gccgcgatgt
    14161 tcttcgccat ggtcggaacc ctgattccga ccacgcaatt tggcggcttg atggacccgg
    14221 tgtcctcact ggaggggggt gcacgcgtca tcggcgagat ctaccccgcc agccacatgt
    14281 tcatcatcag ccgcggcgtg ttcaacaagg cgcttggctt tggcgacctg tcggccaact
    14341 tctggccgct gctgctggcg gtgccggtga tcgtcggcac cgcgatcgcg ctgctccgca
    14401 agcaggaggt ctgagcatgt ggcagaggct gtccaacatc taccacctgg gcgtcaagga
    14461 actgtggagc ctgtggcgcg acccgatcat gctggtgctg accgtgttct ccttcacctt
    14521 cgcggtgtat tcggcggcgt cctcgatgcc cgaaaccctg aacaacgcgc cgatcgccat
    14581 cgtcgacgag gaccagtcgc agctttcgca gcgcatcgtc agcgcgttct acccgccgca
    14641 gttcacggcg ccgttcctga tcgaccagtc gcgggtcgac ccgggcatgg actcgggcgt
    14701 ctacaccttc gccctgatca ttccacccaa tttccagcgt gacgtgctgg ccgggcgcga
    14761 cgcgcaggtc cagctcaaca ccgacgccac ccgcatgagc caggccttca acggcagtgg
    14821 cttcatccag caggtggtgc tgggcgaggt gcaggagttc gtgcagcgct accgcgccgg
    14881 cgccgagccg gcggtgggcc tggagttgcg cgcacgcttc aacccggcgc tggagaagtc
    14941 ctggttcggc ggcctggcgc agatcatcaa ccagatcacc atgctgtcga tcgtcctggc
    15001 cggtgcggcg ctgattcgcg agcgcgagca cggcaccgtc gagcacctgc tggtgatgcc
    15061 ggtgacgccg gccgagatca tgctcgccaa gatctggtcc atggggctgg tggtgctgct
    15121 ggcgtcggcc gtgtcgctcc acgtcgtggt gcgctgggcg ctgggcgtgc cggtggccgg
    15181 ctcgctgccg ctgttcttct tcggcgccgc gctgctgttg ttcgccacca cgtcgatggg
    15241 catcttcatg gccacggtcg cgcgcaacat gccgcagttc ggcatgctgg tgatgttggt
    15301 gctgctgccg ctgcagatgc tctcgggagg tcagtcgccg cgcgagagcc aacccggctg
    15361 gctgcaggac ctgatgctcg ctgcgccgac cacccagttc gtcaagttcg gccaggcgat
    15421 cctgtttcgc ggcgccggca tcgaggtggt gtgggtgcag ctgctggcca tggcactgat
    15481 tggcgcggcg ctgttcgcct tctcgctggc gcgctttcgc aagaccatcg gccagatggc
    15541 gtgatggccg ctgcctgcag gccgacgaca aacgtcttcc atgccgggca ccccgtgggt
    15601 ctgactgcgg cgggtgggtg cccgcccgaa ggagccacgc cgcatggcgc gacgagcacg
    15661 ctgcgggcgc ctgccgtttc taggctcaga ccagcagcgc gttgacccgt cgcacgtagc
    15721 cggccgggtc gtcgggcagg ccgccttcgg ccagcagcgc ctggtcgaac aggacctgag
    15781 ccagatcgtc gaagcgcgca tgaccgtcga gcttcttcac cagcgcgtgc tcggggttga
    15841 tttcgagcac cgggcgggtg tcgggcaccg gctggccagc ctgtttgagc acacgcgcca
    15901 gctgcaggct gatgccgctg tccttgacca ccaggcaggc cggcgagtcg accaggcggg
    15961 tggtgacgcg cacgtcttcc acgctgtcct tgagcacgtc cttgagcttg tccagcagcg
    16021 gcctgacggc ttcggcggcg gcttcggcgg cctgcttttc ggcttcgttc tgcagctcgc
    16081 ccagatccac cgcacccttg gccaccgact gcagcggcgt gccgtcgaat tcgcgcaggt
    16141 aggacagcgc ccactcgtcc acgcggtcgg tcatcagcag gacttcgatg cccttcttgc
    16201 ggaagatttc gagctgcggg ctgtggctgg ccgccgccag ggtgtcggcg gcgatgtagt
    16261 agatggcttt ctggccgtcc ttcatgcgcg ccttgtagtc ggccagcgag acggcgggcg
    16321 tgtcgctggc ggtggaggcg tagcgcagca ggcgggcgat gcgctcgcgg ttggcgtggt
    16381 cttcgcccag gccttccttg agcacggcgc cgaattcgcg ccagaaggtg gcgtacctgt
    16441 cgccggcggg cggcgggttc tgcacgtcgg tggccggctc gccaccggcc gcttcggcgg
    16501 gcgcttcggc gggcgcttcg gcgggcgcct tggccagctc ttccagcatg gtcagcacgc
    16561 ggcgcgtgtt gccttcgcgg atcgaccgca cgtcgcggct ttcctgcagc agctcgcggc
    16621 tgacgttgag cggcaggtcg gccgaatcga ccacgccctt gacgaagcgc aggtagcgcg
    16681 gcagcagctg ctcggcgtcg tccatgatga acacgcgcct gacgtacagc ttgacgccgg
    16741 gcttcttgtc gcggtcccac aggtccatcg gcgcgtggct gggcaggtac agcagctggg
    16801 tgtattcggt gctgccttcg acgcggttgt ggctccaggc cagcggcggc tgccagtcgt
    16861 gcgcgacctg ctggtagaag tcccggtatt gctcgtcggt cacgtccttc ctggggcgcg
    16921 tccagagggc ggtggcctgg ttgacggttt cccagtcccc ggtcttgacc atctggccgg
    16981 gctggccctc ttccttgccg tcttcccact tctcgccttc catctggatc ggcagggcga
    17041 tgtggtcgga gtacttgccg atcagctcct tcaggcgcca gccgtcggcg aattccagcg
    17101 cgtcttcgcg caggtgcagg atcacgcgcg tgccgcgctc gggcagggtg aggcgctcga
    17161 cgctgaagtc gccgctgccg ctgctgaccc agcgcacacc ctgttccggc ggcaggccgg
    17221 cgcggcgcgt ttcgaccgtg atgcggtcgg cgacgatgaa gcccgaatag aagccgacgc
    17281 cgaactggcc gatcagctgc gcgtcctgct tctggtcgcc ggtcaggcgg ctcatgaact
    17341 cgcgcgtgcc gctcttggcg atggtgccca gatgttcgat ggtctcctgc tcgctcatgc
    17401 cgacgccggt gtcgctgatg ctcagcgtgc gggcgttcct gtcgaacgcc acgcgcacgc
    17461 gcagctcggg ttggtcgccg tacaggctgc tgtcggccag cgcctcgaag cgcagcttgt
    17521 cgcaggcgtc ggaggcgttg gaaatcagct cgcgcaggaa gatttccttg ttggagtaca
    17581 gcgaatgggt gaccaggtgc agcaggttgg ccacttcggc ctggaaggca tgggtttggg
    17641 tgctcatggt gcgggatagc cgttcatggg tgaaacaacg ggaggtgcag cgcgcttcgt
    17701 tcgcgtcggt caatgcggcg cgccgtgccg caactggggt tgcggggcgg attttcaagc
    17761 cgatggtgcg ccacgggggc agaatcgcta ttgaaaaagt agctgctggc gctggattga
    17821 agagcgccga atgcgatttt gtcttcggat cgggccgagg ttccggtgcc ggccacgccg
    17881 ccttgcatcg cgttcgcatc cagggcgcgc cgcgtggcag aggcgaagtc ggcgacgcct
    17941 cagcgctcgg cggtggccgt ggccgggtcg cgctcgcccg gcggcgtcag ccagcgcacc
    18001 agctggcgcg ccaccgccgc gtcgctgagc aggtccaggt ggccgcgctg ggcgaccacg
    18061 tactggtcgg cgggggcaaa ggccaggcag cgtgcggggt tgtcgtgcgc gcccagcgcg
    18121 ctgcgcaggg gcaccagccc gtcgccgacc agccgctcgg ccagcgggct gcgctgcgcc
    18181 gccagcgtgg cggccacggc gtggcacgcc accccgtcgg gcagcggcac gggggtgcgg
    18241 gtgtcggggc tgcgggccaa acgatcacgg ccctgccagt cggcgtcgag cacatggccg
    18301 tagcgcaggt cgctgatgcc ggcgctgcgc aggtgcccca ggcgcgtgaa gggacggctg
    18361 tacggggtgc tggccagcag caggtcgacc cacgggccgg cgcgcgccag cggtgcgccg
    18421 tggtgcggcg tgcctagaaa gatcaggtgc ctgagctgcg ccggccagcg ttggccggtc
    18481 tgcgtcgcgt gccacacggc gctgcgcgcc accagcccgc ccatgctgtg cgccaggatg
    18541 ccgagttcct gcaccggcac cggccagctg gccagcagcg cctccagatg ctgggccagc
    18601 gcgcgcccgt tgaccgagat gtgcagcccg gtattgtagc gcaggtagac gggggtgtag
    18661 cccagcgttc gcgccaggtg tgcgccatgg tcatggcccg aacgcagcca ttggcggtcg
    18721 ttcatgcaca ggccatggat cagcagcagc acccggcccg tggccgcgcc ttgcgcgtgc
    18781 aacgccgcca gctcgagtgc gcgcccgtgc tggcgcaggg ccatcggcaa ggccagcggg
    18841 ttgtcgtcgg ccgccaggcg gtcgcccagc acgccgttca gcgccgccag cacggcatcg
    18901 cgctcggcct cggccgactc gtcgggcgcg ccctgcgccc gctccagcca gggttcgagc
    18961 cgccgcagcg cggcctgcag cgcggcgtcc acccccagcg tcacgccctg gatgctgcgg
    19021 tagaccatgc cggtgatgcc gcgcgtgcgg cccggtgcgg ccgcgccggg caggccgagt
    19081 gtgcccagca ccgaccggtg cacgccttcg acgatgcgcg tcacgcccat cgtggcctgg
    19141 gttgccaggc gcgccgcacc gcgcaggtcg gccgcgcgcc accgctttcg cgcggcctgc
    19201 tttggcctgt cgggtccggg cggtggggtc ggggtcatgg tggcaagggc tggctgcggt
    19261 ggggtcagaa gccctcgtgc gcgcccaggt accgccactg accgaccggc agctcgccca
    19321 ggcgcacctg gccgatgcgt acgcgcttga gtccggtgac cttcaggccg accagctcgc
    19381 acatgcggcg gatctggcgc ttcttgccct cgcgcagcac gaagcgcagc tgctcggggt
    19441 tttgccagtc gacctgggcg ggcttgagcg gctggccgtc caggctcagg ccatggcgca
    19501 ggcgcgccag ctgtgcggcc ggaaaggcgg cctgcacgtt ctcggtctgc gcgccgtagc
    19561 tgacgcgcac caggtattcc ttgtcgatcg tcgagtcttc gccgatcaac tggcgcgcca
    19621 cgcggccgtc ctgggtcagc accagcagac cgaccgagtc gatgtccagc cgcccggcgg
    19681 gggccagacc gcgcagctgg gccggcgcaa agcgggtgcg gctggcgtcg ccgcgccaat
    19741 ggctgcgcgc gtggatcagg gtcacggcgg gctggtggcc gtcttcggcc tggccgctga
    19801 cgtagccgac cggcttgtgc agcaggatgg tcacgcgttc ggcctgggcg gcgtcggcgc
    19861 gccggtccac acgcacctgg tcggccgtgg tcaccttcag gcccatcacc gccacctggc
    19921 cgttgacgcg cacccagccg cgctcgatcc agtcgtcggc ttcgcggcgc gaacacagcc
    19981 ccagatcggc caggcgcttg ttgaggcgga tcgacgccgg cgccgcgccg gcggcggcgg
    20041 tgtcggggcg gggcgcacgg cgaaagcggg cggggtcgga ggtcatgggc ggtgttgaat
    20101 actacacaaa taatagctgc tggcgcttgt tggtaaagcg atagaggccg atttgatcaa
    20161 aaatcccttg accgcagcgc ggccagatcg aggatgcgca gcccgccata ttcgatgcgc
    20221 agcagctgct gggccgacag cgattgcagc gcttcgttga cgcgctggcg cgacaggccc
    20281 accaggtagg ccagctcctg ctgcgtgatg cgcagcactt cgcccacgcc gggaaacagc
    20341 accggattga ccagcgccgc gatgctgcgc gccacgcgct gctcgggttt gggcgtgcgg
    20401 tcggcttcca gtgtgccgat gaagtgtccc aaccgctcgt tgagctgatt catgatgaag
    20461 cgattgaagc cgatcgagtg gtcgagcagc cagtggaagg tgtcggccgg cagaccagcc
    20521 accaggctgc gccgcagcgc actgacgttg tagcggtagg gctcgcgctt gatcaccgtg
    20581 ccttcgccga accagccgcc aggcgcgagg cccgagtagg tgatggtctg tccttcggcg
    20641 gtttcggtgc tcatcttcag cagcccgtcg acgacgccga accagtaggt caccgggcgg
    20701 cccctgcggc acaccagctc gcccggctcg gccagcgaga tgcgcagctc gggcgcggcg
    20761 cggtcgaact cctcggcctt gaggtgcgac agccagggca cacccgccag ctcggccgcg
    20821 ttcagcgggc ggcggcgttc atgcagggcg gacggatggg ccatgtgcgg gaaatcccgg
    20881 gtcggatttg cccgcaattg tcgttcaaac gacaacccag cgtcaaagtg gcgccgtacc
    20941 atgcgcgtcg atcgaacgca tgcgcctggg gccacgccag gcgctcgaat cacaggagac
    21001 gcgccccatg gcgaccacct ttccccgcct gctgcagaag cacgccgccg agcgaccggg
    21061 cgatccggcg ctgcgcgaga aggaatacgg catctggcaa acctggagct ggcagcgcgc
    21121 gctcgacgaa gtgcgctggc tggcctgcgg cctgacggcg ctgggcttca cgccgggaca
    21181 gaacctggcc atcgtgggcg acaaccggcc gcacctgtac ctgatgttcc tggccacgca
    21241 gtcgctgcgc ggcgtgccgg tgccgatgta ccaggacgcg gtggccaccg aaatggcctt
    21301 cgtgctgcgc gacgccgaag tgcatttcgc cctggccgag gaccaggagc aggtcgacaa
    21361 gctgctggag ctgcgcgccg ccaccggcga gcaggccgtg ccgggcctga cccacatcat
    21421 ctacgacgac ccgcgcggca tgcgcaacta ccgccagccg ggcctgatca gcgtcgacga
    21481 gctgatggcg ctgggtcggg cgcacgaacg ggagcatccg cagctgttcg ccgacctggt
    21541 ggcccagggc cagcccgacg acgtggcggt gatcctctac acctcgggca ccaccgggcg
    21601 acccaagggg gtgtgccaga cccacgcggc gtttgtcgcc tcggcgcgcg gcggggtcga
    21661 aaccgatggg ctgaaggccg gcgacgacgt gctgtcgtat ctgccgccgg cctgggtggg
    21721 tgacttcctg ttttcgatgg cgcagtggct ggtggcgggt ttcaccatca actgcccgga
    21781 atcgagcgat acggtcggca tcgacatgcg cgagatcggg ccgacgtatt acttcgcgcc
    21841 gccgcgcgtg ttcgagggca tgctgacttc ggtgtcgatc cgcatggaag atgcggcgcg
    21901 cttcaagcag cgcctgcacg cccatttcat gggcgtggcg cggcgcgtcg gctccgatct
    21961 gctggacggc aagccggtgg gcctggccga ccggctgcaa tacgccatcg gcaacctgct
    22021 ggtctacggt ccgctgaaga acgccatggg cctgtcgcgc atccgcgtcg cctacaccgc
    22081 gggcgcggcc atcgggccgg acctgttccg cttctttcgc tccatcggcg tcaacctcaa
    22141 gcagctctac ggtcagaccg aaacctgcgc ctacgtctgc atccagcgca acggtcaggt
    22201 caagctcgaa agcgtcgggc aggccgcgcc cggcatcgaa ctgaagatcg ccgacagcgg
    22261 cgaagtgctg gtcaagggcg tttcggtgct caaggaatac tacaagcgcc ccgacgccac
    22321 cgccgaggtg atcgacgaga acggctactt cagaaccggc gacgccggcg tgctggaccg
    22381 cgacggccac ctgcgcatca tcgaccgtgc caaggacgtg ggcaaactgt ctggcggcgc
    22441 catgttcgcg cccaactaca tcgagaacaa gctcaagttc ttcccgcaga tcaaggaagc
    22501 cgtgtgcttc ggccacgagc gcgagcaggt ctgtgccttc atcaacatcg acttcgaggc
    22561 cgtgggcaac tgggccgagc gccagggcct gccgtatgcc ggctacgtcg atctggccgg
    22621 caagcccgag gtgctggcca tgatgaagga ctgcgtcgag caggtcaacg ccggtctggc
    22681 cgccgaagag ggcatgcagg acacgcagat cgcccgcttc ctggtgctgc acaaggaact
    22741 ggaccccgac gacgacgagc tgacgcgcac gcgcaaggtg cggcgcggct tcatcgccga
    22801 gaagtacggc gtgctgatcg atgcgctcta cagcgggcgc gccgagcagt tcatcgagac
    22861 cgcggtcaaa ttcgaggacg ggcgcgccgg caaggtgtcg gccacgctga agatcctcga
    22921 cgcccgcacc tacccgcccg tccggagcgc ggcatgaaca cgatggctac tgaaacaata
    22981 gctgtcagcg cgcaccagac gggcgctgca gccggatccg atctgcaatc cgggggcaag
    23041 aagatcggcg acgtgatcct cgacgtgcag aacatcagcc tgcgattcgg cggcgtcaag
    23101 gcgctgaccg acatcagctt caacgtgcgc gagcacgaga tccgttccat catcggcccc
    23161 aacggcgccg gcaagtcgtc gatgctcaac tgcatcaacg gcgtctacac gcccagcgaa
    23221 ggcagcatca ccttccgtgg caagaccttc agccacatga acagccgcca ggtcgccgag
    23281 atgggcgtgg cgcgcacctt ccagaacctg gcgctgttca agggcatgag cgtgatcgac
    23341 aacatcatga ccgggcgcaa cctgaagatc aaaagcaacc tgctgatgca ggcgctgcgc
    23401 atcggcccgg ccgagaggga agagatcgca caccgcgagt tcgtcgagca catcatcgac
    23461 ttcctggaga tccaggcgca ccgcaagact ccggtgggcc agctgcccta cggcctgcaa
    23521 aaacgcgtcg acctgggccg cgcgctcgcc atggagccgc aggtgctgct gctggacgag
    23581 cccatggctg gcatgaacgt cgaggaaaag caggacatga gccgcttcat cctcgacgtg
    23641 aacgacgagt tcggcaccac catcgtgctg atcgagcacg acatgggcgt ggtgatggac
    23701 atcagcgacc gcgtggtggt gctggactac ggcaagaaga tcggtgacgg cacgcccgac
    23761 gaagtgcgcg gcaacgagga cgtgatccgt gcctacctgg gcaccagcca ctgagcgcgc
    23821 gaggaagcac aaccatggga ttctttctgg aaacgctgtt cggcggcctg atgacgggca
    23881 tgctgtattc gctggtggcg ctgggcttcg tgctgatctt caaggcctcg ggcgtgttca
    23941 atttcgccca gggcgcgatg gtgctgttcg cggcgctggc catggcgcgc ctggccgagt
    24001 ggctgccgca gtggttgggc ttcgaaaacc gggtgctggc caacctgctg gcctttgcgc
    24061 tggcggcgct gatcatgtgg gccgtggcct ggctgatcga gcgcctggtg ctgcgccatc
    24121 tggtcaacca ggaaggcgcc acgctgctga tggcgacgct gggcatcacc tactttctgg
    24181 acggcctggg ccagacgctg ttcggcagcg ccagctacca gatcaacatc ggcatgccca
    24241 aggacccgat gttcctgttc gagaacgtct tccagggcgg cgtgctggtg aacaaggaag
    24301 acctggtggc ggcgctgatc gccgccgcgc tggtgaccgg tctgaccctg ttcttccaga
    24361 agaccaagac cggccgggcg ctgcgcgcgg tggccgatga ccaccaggcc gcgcagtcga
    24421 tcggcatccc gctcagccgc atctgggtca tcgtgtggac ggtggccggc gtggtggccc
    24481 tggtggccgg catgatctgg ggcagcaagc tgggcgtgca gttctcgctg gccacggtgg
    24541 cgctgcgcgc gctgccggtg gtgattctgg gggggctgac cagcgtgccg ggggccatcg
    24601 tcggcgggtt aatcatcggc gtcggcgaga agctgtccga ggtctacctg ggcccgttcg
    24661 tcggcggcgg catcgagatc tggtttgcct atgtgctggc gctggtgttt ctgctgttcc
    24721 ggccgcaggg cctgttcggc gaaaaaatca tcgaccgcgt ttgacatcgc tcctgttccg
    24781 atagcttctg gcgcgcgttg aataagccct gaccaccgat tcgagaccca ccatgttcta
    24841 cagagaaaac ggccagttca agaccagcta ccgagccgac cagcagatct tcccgatcgc
    24901 ccaggatcgc tgggcggtgc tgctgctggt ggcctgtgcc ttcgtggtgg tgccgttgct
    24961 ggccagcgaa tacctgttcc tgtcggtgct gatcccgttt ctgatcatgg ccatggcggc
    25021 catcggcgtg aacatcctgg tcggctactg cggccagatt tcgctcggtt caggcgcctt
    25081 catggcggtg ggcgcctacg cggcctacaa catgtatgcg cgcatcccgg gcatgcagat
    25141 gctggtggcg gtgctgaccg gcggtgcctt tgcggccctg gtgggcatcg tgttcggcat
    25201 tcccagcctg cgcgtgcgcg gcctgtacct ggcggtggcc acgctggcgg cgcagttcgt
    25261 gtgcgattgg gccttcctgc gcatcagctg gttcaccaac cacagcacct ccgggtcggt
    25321 gtcggtgtcc aaccttcatg tgctgggcct gccggtggag acgccggcgc agaagtacct
    25381 gttctgcctg agcctgctgg tggtgttcgg cctgctggcc aagaacctgg tgcgcagcgc
    25441 cgtggggcgc gaatggatgg ccatccgcga catggacgtg gccgcggcgg tgatcggcat
    25501 ccggcccatg tacgccaagc tgagcgcgtt cgcggtctcc agcttcatcg tgggcgtggc
    25561 gggggcattg tgggggttcg tcaacctcgg ctcgtgggag ccggccgcct tttcggtcga
    25621 tcgctcgttt cagctgctgt tcatggtgat catcggcggt ctgggctcga tcctgggttc
    25681 gatcctgggt gccgcgttca tcatcgtgct gccgatcgtg ctcaatctgc tgctgccctg
    25741 gctgggttcg ctgttcgggc tcgaggtgtc gatcgccgcc gtcgagcaca cccaggtgat
    25801 gctgttcggc gcactcatcg tctggttcct gatcgtcgag ccgcatggcc tggccaagct
    25861 gtggagcgtg ggcaagcaga agctgcgcat ctggcctttc ccccactgac cgggcaccac
    25921 agcgcccgca aacccaacca ccgttttttt cctaggaggc aaaccatgaa gttccgtcaa
    25981 ctgacccttg ccgccgcggc ggccctcacg ctgggcgcct cggcgtcggc catggcccag
    26041 gcacagcagt tctttccggt gctcagcggc cgcaccgggc cggtggcgcc caacgccacg
    26101 ccctgggcca acggccacaa cgactacatg aaactggtca acgcgcgcgg cggcatcaac
    26161 ggcgtcatga ccctggtgga agaatgcgaa accgcctacg ccaccgacaa gggcgtggaa
    26221 tgctatgaac gcctgaaggg caagcacggt ggcgccacgg tgttccagcc gctgtccacc
    26281 ggcatcacgt tcgcactgac cgaaaaggtg ccgggcgaca agatctcgct catcacctcc
    26341 ggctatggcc gcagcgacac cgccgatggc ggcgtgttca agtggaactt cccgctcatc
    26401 ggtcactact gggtggcggg cgacacggtg ctgcagcaca tcgccaagaa ggaaggcggc
    26461 tgggacaagc tcaagggcaa gaagttcgcc atcgtctacc acgacagccc gttcggcaag
    26521 gagctgctgc cgatcgtgca ggagcgcgcc aagatgcacg gcttcgagct gcaactgctg
    26581 ccggtgccgg cccctggtgt cgagcagaag gcgatctggc tgcaggtgcg tcagcagcgg
    26641 cccgatttcg tcatcatgca gacctggggc gtgatgaccc ccaccgccat caaggaggcc
    26701 gtggccaccg gctatccgcg cgagaaaatg ttcggcacct ggtggtcggg cgccgagcct
    26761 gacctgaagg acgtcggcgc cgccgccaag ggctacaacg ccgtcatgat gcagcacggt
    26821 gccgagccgc agtccgccgt ggtgaaggaa atcctcgaga aggtgcacgc caagggccag
    26881 ggcaccggtc ccaaggaaga ggtgggcagc gtgctgtaca tgcgcggcgt gatgggcgcc
    26941 atgctggcgg tcgagggcgt gcgcgcggcc caggagcgct tcggcaaggg caaggtcatg
    27001 accggcgagc aggcacgctg gggctatgaa aacctgaacc tgacccaggc caagctggac
    27061 gcgctgggct tcaagggcgt gatgcgcccg gtgtccacca gctgccagga ccacatcggt
    27121 tccggctggg cgcgcgtgca cacctgggac ggcagcaagt tcgtctggtc ttccgactgg
    27181 ctggaggccg accagcagat catcaagccg atggtcaagc agtcggccga caaatacgcc
    27241 gccgagaaga agctgacccg ccgcaccgcc gccgactgcg gcagctgagc cagcggcagg
    27301 agcgagccgc cgcccaccgc agggtgcgcc cgcacgcgcg tgcgggtggc gctgcggcgc
    27361 tcgctgcacc ggcgttcgcc cgtcgcgcac gggcgccatt ggagcctaga tcatgaccac
    27421 cgcatccgcc cccatcgtgc tgaccgtcaa cggcatcgaa gtcatctaca accacgtcat
    27481 cctggtgctc aagggggttt cgctgacggt gccggaaagc ggcatcgtcg ccatcctggg
    27541 cggcaacggc gccggcaaga cgaccacgct gcgcgctgtc tccaacctgc tcaagggcga
    27601 gcgcggtgaa gtgaccaagg gttcgatcga gctgcgcggc gagcgcatcg agaacctctc
    27661 gccggccgaa ctggtcaagc gtggcgtggt gcaggtgatg gaagggcgcc actgcttcgc
    27721 ccacctgacg atcgaggaaa acctgctgac cggcgcctat acgcgcagcg acaagggcga
    27781 gatcgccgcc aacctggaca aggtctacac ctacttcccg cgcctgaaga cccggcgcac
    27841 cagccaggcg gcctacacct cgggtggcga gcagcagatg tgcgccatcg ggcgcgccct
    27901 gatgtgcaac cccagcatgg tgctgctcga cgagccctcc atggggctgg cgccgcagat
    27961 cgtcgaagag gtgttcaaca tcgtgcgcga cctcaacacc aaggagcgca ccaccttcct
    28021 gctggccgag cagaacacca acatggcgct gaagtacgcc gactacggct acatcatgga
    28081 aagcggccgc atcgtgatgg acggcaaggc cagcgacctg gccagcaacg aggacgtcaa
    28141 ggagttctac ctcggcgtcg gtggcggcga gcgcaagagc ttcaaggacg tcaagagcta
    28201 caagcggcgc aagcgctggc tggcctgagc gtcatccgac aggcgtgcgt cgcagtccag
    28261 atatctgagt caaatcgaac gctggcgctt gtggatgctg cgccgacagc tccagttttt
    28321 agagcattca tcatgaccga atactttgac gcgctcgaaa cccgcgaccc cgcggcgcgt
    28381 gaggcggcgc tgctggcggc gctgccccgg cagctggcgc atgcccagca gcacagcgcg
    28441 gcgttcgccg acattctggc cggcgtcgat gccgccgcgg tgacctcgcg cgccgcgctg
    28501 gcgcgcctgc cggtgacgcg caagagcgag ctgctggcgc gccagcaggc cagccgcgcc
    28561 gctggccgtg acccgttcgg cggttttgcc accgggcgct gggggtcggc catgcggcgc
    28621 gtctatgcca gcccgggccc gatctacgaa cccgagggct gcgccagcga ttactggcgc
    28681 accgcgcgcg cgctgtacgc ggccggcttt cgcgccggcg atctggtgca caacgccttc
    28741 agctaccaca tgacgccggg tgccttcatc ctcgaagccg gggcgctggc ggtaggctgc
    28801 acggtgtttc cggccggcac cggtcagacc gagcagcagt tgcaggccat cgccgagctg
    28861 cggccgcagg cctacgtcgg cacgcccagc tttctgcgca tcctgttcga gaaggcggcc
    28921 gagggcggcg ccgacatccg cagcctgacc aaggccatgg tcagcgccga accgtttcca
    28981 ccctcgctgc gcgactggtt cgccgagcgc ggcatgacgg cctaccagag ctacgccacc
    29041 gccgacgtcg gtctgatcgc ctacgagacg gcggcgcgtc agggcctggt gctggacgag
    29101 ggcgtgatcg tcgaaatcgt acgccccggc accggcgacc ctgtgcccga gggcgaggtg
    29161 ggcgaagtgg tggtcacggt gctgaacccg gactacccgc tgattcgctt tggcaccggc
    29221 gatctgtcgg cggtgctggc cggcccgtgc cccaccgggc gcaccaacac ccgcatcaag
    29281 ggctggctgg ggcgcgccga ccagaccacc aagatccgcg gcatgttcgt gcacccgggc
    29341 caggtggccg agatcgtcag gcgtttcccg ggggtggggc gagctcgcct ggtggtcagc
    29401 ggcgccatgg ccaacgacca gatgacgctg cgcgtcgaag cggccgacct gcccggtctg
    29461 gccgagcgca tcgccgacgc cgtgcgcgac gtcaccaagc tgcgtggcca gatcgagctg
    29521 gtcgccccgg gcagtctgcc caacgacggc aaggtgatcg aggacgcgcg cagctacgaa
    29581 taaaccgcgg ggatgcgctt ttttggcgcg gcctcgggga attcccggtg cgcgcggggc
    29641 tggcccaggc gtttcgaatc gataccattg gccgtttcat tccctcattg caaggagctg
    29701 ctgtcatgtc acccctgttc aagctgagca tcgccaccgt cgccgccctg gctgcgctgg
    29761 gcgcccaggc ccaggccttc ccggccgcgg gcaagaccgt caccctgatc gtcccctttg
    29821 ccgccggcgg ccccaccgac aaggtggcgc gcgacctggc cgaggcgctg cgcaagccgc
    29881 tgggcggtgc caccgtggtg atcgacaacg cggccggcgc cggcagcacc atcggcgccg
    29941 ccaaggcctt ccgcgccgca ccggatggct acaccctgct ggtcacccac atcggcatgg
    30001 ccaccacgcc ggcgctgtac cgcaagctgc cctacaacgt cgaggagttc gagtacctgg
    30061 gcctcatcaa tgaggtgccg atggtggtgg tcagcaagcc gggcctgccg gccaacaact
    30121 gggccgagct gaaggcctgg atcggcagca acaagggcaa ggtcaatctg gccaacgccg
    30181 gcctgggcgc cgcctcgcac ctgtgcggcc tgatgctgca acaggcgctg gccaccgaaa
    30241 tgaccaccat cgcctacaag ggcacggcgc cggccatgac cgacctgatg ggcggccagg
    30301 tcgacatcat gtgcgaccag accaccaaca ccaccagcca gatcgagggc aagaagatca
    30361 aggccttcgc ggtcaccacg cccaagcgca tcaccatgcc gccggtctac aaggacctgc
    30421 cgacgctgga agaggtgggc atgaagggct tcaacgtctc gatctggcac ggcctgtacg
    30481 cgcccaaggg cacgccggcg gcggtggtca aggtgctcaa cgacgccctg caggcggcgc
    30541 tcaaggacgc cgacttcatc aagaaggaag aagccctggg cgcggtggtg gcgcacgacg
    30601 atcgcgtgac gccggccggc cacaagaaat tcgtcaccgc cgaagtcgcc aagtggggtg
    30661 cggtgatcaa ggcggccggg cagtacgccg actgagaccg ggcggcgcgg cccacgcccg
    30721 gccgcgtggc gggcgtggcg cccaccaggc ggcaccatga accgacctct gcggctggtg
    30781 gactcgatca ccgagctcag gcccggcgcc gacaccggct gcctggcggt cagcggctcg
    30841 cacgggggca tcagctcggc gcactacgcg ctggcggcgc agccgatgct ggccgtcttc
    30901 aacgatgccg gcgtgggcaa ggacgccgcc gggctggcgg ccctgccgtt cctgcagcgg
    30961 cacggcatcg ccgcctgcac cgtggcccac accagcgcgc gcatcggcga ggcgcgcagc
    31021 accctgcacg acggtgtggt cagccactgc aacgcccagg cgctaagcct gggcatcgag
    31081 ccggggcagg cgtgcgccgc gctgatccgg caacttcaag gctctggagg atgaacatgg
    31141 cacaaggttt caaagcaaat cggcgtctgg cgcttgccgg gattgcgctg gcagctacgc
    31201 aatttctagc aaacatggcc tgggcacagg gcgcctggcc gagcaagccg gtcaggatcg
    31261 tggtgccgtt cgcccccggc ggcaccaccg acatcctggc gcgcgccctg gcgccggagc
    31321 tgagcaaggc gttcggccag cagttcatcg tcgacaaccg cgccggtgcc ggcggcaacg
    31381 tcggggccga ggtggtggcc aagtcgccgg gcgacggcta caccctgctg atgggcacgg
    31441 tcggcacgca cggcatcaac cgtgcgctct accccaggct gccctatgac ccgatcaagg
    31501 acttcgcccc catcaccctg gtggccagcg tgcccaacgt gatggagatg aacgccgaca
    31561 aggcggcgca gctgggcatc cacaacgtgc aggacttcat ccgctacgtc aaggcccacc
    31621 ctggcaggct gaacatggct tccagcggca acggcacctc gatccacctg gccggcgagc
    31681 tgttcaagca catgaccggg gtcttcatga cccacattcc ctaccgtggc tccggcccgg
    31741 cgctgctcga catggtggcc ggcaacatgg acgtgatgtt cgacaacctg ccatcgtcga
    31801 tggcgcagat caaggccggc aagctcaagc cgctggccgt gaccagcgcc cggcgctctg
    31861 ccgccctgcc cgacgtgccg acggtggagg aggccggtgg cccggccctc aagggttacg
    31921 aggccagcag ctggttcggg ctgctggcgc cggccggcac gccggccgat gtggtgagcc
    31981 gactgcagca ggaatcggcc aaggcgctgg cctcgccggc cgtgaaggaa aagctgctgg
    32041 cccagggcgc ggtgccgggt ggcattccgc ccggcgaatt cgccctcatg attgccgacg
    32101 aacacgccaa gtgggccaag gtggtcaagg cctcgggcgc caaggtcgat tgagcgcggc
    32161 ggcggcccgg gccggccgct cgataggcgg ggtggggccg gagggccgcg ccgtcagacc
    32221 ccccacacca cgtctttgcc ggccgcgctg ctgatctgca gcagctcgat gaacggcgcg
    32281 gcgcgctgac gcagcgtcac cagctcctgg cgtgcaaatt catcggcggt ggcctgcgtg
    32341 cgcgcctcgt cgtcgctggt cggtgcggcg tgcccctgtc cctgcgcctc gcggcgctcg
    32401 atctcggcct tcagggcggc gatcgcggcc ggcgcctgat ccacggtgac gatgccggtc
    32461 gggctcgcgg gtttgccgat gatcttgagc agctcttcgg ccgaggcctt gagcatgatg
    32521 acgtcggcgg cggcctgcga cttgaatttg taaagcatgg ctggggtctc cggttgatgg
    32581 cgcgcccatg gcgcctgtat ccgattgtgc cccgtccggg taactaccaa gccgcccgac
    32641 gtcaaatcct cttgacctga tttagttgag acataaacta tttttgcatc aaccaaatcg
    32701 gctgtggctc aggagacgga gcatgaagat tgtgtgcatc ggaggcggac cggcgggcct
    32761 gtacttcgca ctgctgatga agcggctcaa cccggcgcat gacatcacgg tgatcgagcg
    32821 caaccggccc tacgacacct ttggctgggg ggtggtgttt tccgatgcca ccatggacaa
    32881 catgcgccag tgggacgccc ccacggccga cgccatccag caggccttca accactggga
    32941 cgacatcgag ctgctgttca aggggcaggt gatccgctcc ggcggccacg gcttcgtcgg
    33001 catcggccgc aagaagctgc tcaacatcct gcaggcgcgc tgcgaggcgc tgggcgtgcg
    33061 gctggtgttc gagcaggagg tcgacgacga tgccgcgttt gccgacgccg acctgatcgt
    33121 ggccagcgac ggcatcaact ccagaatccg cacgctgcat caggcgcagt ttcgacccga
    33181 actggtggtg cgcccgaacc gctacatctg gctgggcacc cgcaagctct acgacgcctt
    33241 caccttcatt ttcgagaaaa ccgaacacgg ctggttccag gcccacatct acaagttcga
    33301 ccaacacacc tccaccttca tcgtcgaatg ccccgagccg gtgtggcggg cgcacgggct
    33361 ggaccaggcg acgcaggagg cgtcggtggc gttctgcgaa aagctgttcg cccgccacct
    33421 gcagggggag cgcctgatga ccaacgcgcg ccacctgcgt ggctcggcct ggctcaactt
    33481 cacgcgcgtc aagtgcgacc gctggtggct gcgcaacgcc aacggggccc atgtggtgct
    33541 gatgggcgac gccgtgcaca cggcgcattt cgccatcggc tcaggcacca agctggcgct
    33601 ggaggacgcc atcgggctga cgcgcgagtt caagcgcctg ggcgacagcc cggcacagat
    33661 cgagcaggtg ctaagcgact accaggcgtc gcgcagcgtc gaggcgctgc gcctgcagaa
    33721 cgccgcctgg aacgcgatgg agtggttcga ggtctgtggc gaacgctact gcgaccagct
    33781 ggagccgccg cagttcatgt attccatgct cacgcgcagc cagcgcatca gccacgagaa
    33841 cctgcgcctg cgcgaccggc agtggctgga aggctatgag cgctggttcg ctaggcgcga
    33901 cggtctggcc gtggccgatg ccgcccggcc gccgccgccg atgttcaccc cctacaccgt
    33961 gcgtggtgtg acgctgaaga accgcgtggt ggtgtcgccg atggcgcagt actcctgcgt
    34021 cgacggcgtg ccgggcgact atcacctggt gcacctgggc gcacgcgccc tgggcggcgc
    34081 cggcctggtg atggccgaga tgacctgcgt cagccccgaa ggccgcatca cccccggctg
    34141 tcccggcctc tacaacgccg agcagaaagc cgcctggcag cgcatcgccg actggatcca
    34201 cgagcacagc gacgccaggt tcggcatgca gatcggccac gccggcccca aggcctcgac
    34261 ccgcctggcc tgggacggca ccgacctgcc gctgccgcag ggcaactggc ccatcgtctc
    34321 ggcgtcgccg cagcagtacc tgccgggcat cagccagcgg gcccgggcgc tgacgcaggc
    34381 cgaaatgcag gtcatcctgc agcagttcgt gcaagccgcg cagagcgcgg ccgacatcgg
    34441 cgtcgactgg ctggagctgc actgtgcgca cggctacctg ctgtcggcct tcatctcccc
    34501 gctcaccaac cagcgcacgg acgaatacgg cggtagcctg gaaaaccgcc tgcggtttcc
    34561 gctcgaagtg ttccgggcga tgcgtgccgt gtggccgccg cagcggccaa tgtcggtgcg
    34621 catctcggcc accgactggg tcgaaggcgg caccacgccg gccgatgcgg tggccatcgc
    34681 gcgcgccttc aaggaggccg gtgcggatct gatcgactgc tccagcgggc aggtcagcaa
    34741 gcaggagcaa ccggtgttcg gccgcatgtg gcagacgccg ttcgccgacc gcattcgcca
    34801 ggaggcaggc atcgccacca tggccgtcgg tgccatcagc gaagccgacc atgtcaacag
    34861 catcatcgcc gccggccgcg ccgacctgtg cgcggtggct cggccgcacc tggccaaccc
    34921 ggcctggacc ctgaccgagg cggcccgcat cggctacgcc gacatgacct ggccacgccc
    34981 gtatttccag ggcaagcgcc agctcgaagc cgggttcgaa cgcgcccggg ccgccggcgg
    35041 cgccccgatc tgaggaagcg acgatggacc tggaagcgcg cgcccacgcc gaacaccccg
    35101 aagcgctgcg cctgtggctg cgcctgctga cctgcaccca gatggtcgag cgcgacctgc
    35161 gcaaccgctt gcgcgaggcg ttcgacacca cgctgccgcg cttcgacctg atggcgcagc
    35221 tcgaacgcgt gcccgagggc ctgcgcatga acgagctgtc acgccgcctg atggtcaccg
    35281 gcggcaacgt caccggcatc accgatcagc tggaggccga aggcctggtc gagcgcgtgc
    35341 cggtcgaagg cgaccggcgc gcctttcatg tgcggctcac gccggccggc cgccagcagt
    35401 ttcaggccat ggcccaggcg cacgagcaat ggatcgtcga tgccttcgcc ggtctgacct
    35461 cggccgagat ccgcaccctg caccagctgc tgggccgggt gaagcaacat tttctggaga
    35521 ccacgtgaag cactacatcc cttcagccaa cccgatgcgg ccgcagtacc gggcgcaggc
    35581 ggcctaccag gcgcgccact ttgcctggca gtgcgacgcc gacggcgtgg ccaccgtcac
    35641 gctcgatcgt cccgagcgca agaacccgct cacgtttgac agctacggcg agctgcgcga
    35701 tttgttcaat ggcctgcgcc acgccaccga cgtcaaggtg gtggtggtga cgggggcggg
    35761 cggcaacttc tgctcgggcg gcgacgtgca cgacatcatc gggccgctga ccaagatgag
    35821 catgcccgag ctgctggagt tcacgcgcat gacgggtgat ctggtcaagg ccatgcgggg
    35881 ttgcccgcaa cccgtcatcg gcgccatcga cggcgtgtgc gccggcgccg gcgccatgat
    35941 ggcgctggcc tgcgatctgc gctacggcac ggccgccacc aagaccgcct ttctgttcac
    36001 tcgcgtcggc ctggccggcg ccgacatggg cgcctgcgcg ctgctgccgc gcgtgatcgg
    36061 ccagggccgc gccagcgagc tgctgttcac cggccgcgcg atgaacgcgg acgaaggcct
    36121 ggcctggggc tttttcaacg ccttgcacgg cagtggcgaa ctgctggtca aggcccagga
    36181 gatggcgcgc cagctggctg cggggcccat gtttggccac gccatgacca agaacatgct
    36241 gcagcaggaa tgggccatga cgatagatca ggcgatcgaa gccgaggccc aggcccaggc
    36301 catctgcatg cagacccagg acttccgccg cgcctacgaa gcctttgccg ccaggcaaaa
    36361 gccggtgttc gggggtgact gatgagcacc ggcatcctgc aacccacggc tttgcggccg
    36421 tccaccagtc acctgggcct gcccttcttc gatgaagcgc atcggcgcat tgccgccgag
    36481 ttgcccgact ggaccaatgc ccagggtgtg gacgaaagcg acgaccgcgc cgcctgccgc
    36541 gactgggtgc ggcgcctggg cgcgggcggc tggctgcgct actgcgtgcc gggcgactgc
    36601 ggcggcgcgc tgccccagct cgattcgcgc gccctggtgc tgctgcgcga gaccctggcc
    36661 ttttattcgc cgctggccga ctttgccttt gccatgcagg gcctgggcag cggggcgatt
    36721 tcgatcggcg gctcggccag ccagcgccag gcctatctgc cgcaggtggc ccgcggcgcc
    36781 aggatcgccg cctttgccct gtccgagccg gaggccggct ccgacgtcgc
//
LOCUS       DAOOOG010000009        33712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_1069_length_33712_cov_23.185845, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000009 DAOOOG010000000
VERSION     DAOOOG010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 33712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33712
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1069_length_33712_cov_23.185845"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PK242_01630"
     CDS             <1..734
                     /locus_tag="PK242_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ92754.1"
                     /translation="VAGAAPPASMIEGMERLGFDLTHVYGLTEVYGPATVCAKHESWE
                     QLPIGERAALNARQGVRYHLQRDARVLDPETLQPVPMDGETMGEIMFRGNIAMKGYLK
                     NPGATEEAFRGGWFHSGDLAVQYPDGYIKIKDRSKDIIISGGENISSIEVEDVLYRHP
                     AVLAAAVVAKPDPKWGETPCAFLELKPGAEVSEADIVAHCKQHLAGFKVPRAIVFGEL
                     PKTSTGKIQKFELRKQAGSAAAIDV"
     gene            complement(834..1202)
                     /locus_tag="PK242_01635"
     CDS             complement(834..1202)
                     /locus_tag="PK242_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HOZ92755.1"
                     /translation="MSADALPQWLQWLIAGLLLASGVFALAAAWGLVRLSSFYQRMHP
                     PALVYVGSSWCVTLASILFFSGQQQGPQLHVWIIIILLSITVPITTVLLSRAALFRHR
                     VAGRTDVPEALRPQAPAPQD"
     gene            complement(1199..1477)
                     /locus_tag="PK242_01640"
     CDS             complement(1199..1477)
                     /locus_tag="PK242_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HOZ92756.1"
                     /translation="MSPLLSGALALTLGAYAVAMLIALWRVLRGPTAQDRVLALDMIY
                     VIAMLIMLVLGIRHASDLYFEAALLIALFGFVGSAAMAKFLLRGEVIE"
     gene            complement(1474..1965)
                     /locus_tag="PK242_01645"
     CDS             complement(1474..1965)
                     /locus_tag="PK242_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOZ92757.1"
                     /translation="MRRWLPAPLLTAALILLWLVLNRPFSLGQLPLALLFGVAAPALL
                     APLRPARPRIRHPLKVLRLILTVGHDVLVSSLDVFWTLVRNRSRPVHSRFVVIPLTLR
                     DPTALASLTMITTVVPGTVWCELARDCSAMLLHVWHVPDEAEFVAFFKRRYEQPLKEI
                     FES"
     gene            complement(1962..3641)
                     /locus_tag="PK242_01650"
     CDS             complement(1962..3641)
                     /locus_tag="PK242_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HOZ92758.1"
                     /translation="MPHLVVAPILLPMLTAALMLLLGDTRRPLKAALNVLASAAGVLV
                     AAALVLQVQRSGAAQSIGVYLPGNWPVPYGIVLVADRLSAMMLLLGSLLGLAGVLYAS
                     ARWHRAGVHFHPLYQLQLMGLAGAFLTADLFNLFVFFEIMLTASYGLLLHGSGWPRVR
                     AGLHYIAINLMASSLFLIGVAMLYGITGTLNMADMAIIVPTIAEDDRGLLHAGAAILA
                     IAFLVKAAAWPFNAWLPAAYASAAAPVAALFAVMTKLGVYAILRLWTLLFPQDAGISA
                     LFGGPVLIGLGMLTLGFGVIGVIAVQQLGRMAAFCAIVSSGTLLAAIGFGRPAVTGGA
                     LYYALGSTLAVGAFFLLVELIDRARDVEEQPVYVGESDPDDEPFAFPVDLVPTHGVNL
                     DDDEEALIGRAIPAAMAFLGLAFIVCALTIAGLPPMAGFVGKVVMLSALLNPAGFGVQ
                     APVSPAAWLLLALLVTGGLLTIIAMSRAGIRCFWAPAGRPPPRLRIIETLPIALLLLP
                     IGVLVWQADAVLRYANATARHLHDPRDYIGSVMAARPQPSPHASDQGGLRR"
     gene            complement(3668..4060)
                     /locus_tag="PK242_01655"
     CDS             complement(3668..4060)
                     /locus_tag="PK242_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HOZ92759.1"
                     /translation="MEVILALAIGVLGGCGVWLVLRPRTFQVLMGLSLLSYAVNLFIF
                     AMGSLFEDKPPIVQPGLTPSLEHYTDPMPQALVLTAIVIGFATSALFLVVLMAARGLA
                     DNDHVDGDNVDDNTALPLRRDDAGGPPR"
     gene            complement(4062..6989)
                     /locus_tag="PK242_01660"
     CDS             complement(4062..6989)
                     /locus_tag="PK242_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HOZ92760.1"
                     /translation="MDLIYLLLLPFAGSLVAALLPTHARNAAAGWAALVGAAALVWVA
                     TRYPALQGGAVLRQELRWLPSAGIDFSLRMDGFAWMFALLVTGIGTLVALYARYYMSR
                     EDPVPRFFSFFLAFMGAMLGVVLSGNVVQLAFFWELTSLFSFLLIGYWHHRKDARRGA
                     RMALTVTGAGGLALLAGVLLLGHIAGSYRLDDILGAGEQIRAHRLYVPMMLLVLLGAL
                     TKSAQFPFHFWLPHAMAAPTPVSAYLHSATMVKAGVFLLARLWPALAGSEAWFWIVAS
                     AGLLTLLLGALLAMFQNDLKGLLAYSTISHLGLITLLLGLGSELAAVAAVFHIMNHAT
                     FKASLFMAVGIIDHETGTRDLRRLSGLWQAMPITGTLAFVASAAMAGVPLLNGFLSKE
                     MFFAETVHLSRNPTLDIGLPVVATLAGVFAVVYSLRFAVGVFLGPPARDLPLTPHEPS
                     RWMRAPVEVLVLVCLLVGVLPQWAIGAALELAARPVVGGAMPAYSLAIWHGINKPLLM
                     SLIALVGGVAMFLWGRQRLAAGDLRLLALPRRLDGQRLFQRLLLGLDRASRGLLRRLS
                     TPRLQPQLLVVVLAAIVLGAAALWPTGISWGERERVPGSAAFALLWVLGGLAALGTAH
                     QAKFHRLAALIMLGAVGLVVSLSFVWLSAPDLALTQLTVEVVTTILFLLGLRWLPKRM
                     PQPGQRLHPRVRARRLRDLLVAGVAGTGMAALAYAMMTRPAPQSIAPFFIDRALPEGG
                     GNNVVNVMLVDFRAFDTLGEITVLGIVALTVYALLRRFRPPREMATLPPQQRAIASDL
                     VSDLINPRTAQDTALGYMLVPAVLVRLLLPVAAVIAMHLFMRGHNQPGGGFVAGLVVA
                     IAFIAQYIVAGTLWVEAHLQLKVIRWIALGLLLAALTGLGSLPLGFPLLTTHTAHLHL
                     PLLGDVHLPSALFFDLGVFAVVVGATLLILTALAHQSVRAYRQAPETMATDAPAHRLP
                     EVG"
     gene            7162..7839
                     /locus_tag="PK242_01665"
     CDS             7162..7839
                     /locus_tag="PK242_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ92761.1"
                     /translation="MRMLLVEDDAMIGEVVLDALRAEHHAVDWVRDGAMALTALATAP
                     YDLLLLDLGLPRQDGLSVLRQLRADHQRLPVLIATARDAVADRVAGLDAGADDYIVKP
                     YDLDELLARVRALLRRADGRAEPAYEWGEVRIVPSTREVTLRGQPVQLSAREWAVLEP
                     LLARPGRVLSRQQLEDKLYAWRDEVSSNAVEVYIHGVRKKLGAGLIENVRGLGYLVPV
                     APPESGS"
     gene            7899..9236
                     /locus_tag="PK242_01670"
     CDS             7899..9236
                     /locus_tag="PK242_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ92762.1"
                     /translation="MKTPAPSLRARLTALLLLAVLLTAALQAAITWRTALAETEAVFD
                     AQMERIALSLTGGLAASVLGDDALAATRGAEELIIQVWRADGIMLYRSPSARLLPPQT
                     VIGFSHLRAGDEDWRVYALETPSQVIQIAQAERARRRIAGQLALRAVLPVALLAPLLM
                     LIVWWVVGRALAPIERLRRQLAARGAADLAPLPTDGLPAEVLPLVTGMNGLLARLSAA
                     WQQLADFTADAAHELRSPLAALRLQVQSLQRADTEQARAVATGRLLAGVDRATRLVEQ
                     LLALARHDGDADAVREPVDLPVLARQALQEADAEAQARGIALRLEGDASLWLDARPQA
                     LAVLLRNLIDNALRHTPAGGQVCVAVQRRPDGVAELAVEDSGPGIPAAERQRVLDRFY
                     RVPGAAGHGSGLGLAIVQAVARRHGAQVVVQASPRLGGARLAVIWPAAAGPED"
     gene            9396..10886
                     /locus_tag="PK242_01675"
     CDS             9396..10886
                     /locus_tag="PK242_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOZ92763.1"
                     /translation="MNRLTLKSSRLFAALLAAGVIGGASVAAVQELTPAQAQPSAAVQ
                     AVAPAAVATPDFAAIAARYGGAVVNISTRGVQPVADEGADSGPGARIDPGLQDFLRRF
                     GLPPGAFQFAPPGPGGPGAVPMHGQGSGFIVSPDGVILTNAHVVRNARTVNVKLTDRR
                     EFQAKVLGRDDKTDVAVLKIDATGLPTVPLGSSQALRPGDWVLAIGSPFGFENTVTVG
                     VVSAKGRSLPDDSAVPFIQTDVAVNPGNSGGPLFNARGEVVGINSQIYSRSGGYQGVS
                     FAIPIELARHVEQQLVAHGKVEHARLGVTIQSVNQALADSFKLPKPEGALVAQVEPGS
                     AAERAGLKAGDVILRANGQAIVDSGDLPARIALARPGDKLALEVWRQGRPEQLSATLQ
                     GARPADTALAQGSAASHGKLGLSLRPLQADEKRQTRLDEGLVVEQASGAAALAGVQPG
                     DVVLSINGQPASSVEKLRAVLAKADKSVALLIERDGQQLFVPVPLA"
     gene            complement(11180..11647)
                     /gene="comJ"
                     /locus_tag="PK242_01680"
     CDS             complement(11180..11647)
                     /gene="comJ"
                     /locus_tag="PK242_01680"
                     /inference="COORDINATES: protein motif:HMM:NF022486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComJ"
                     /protein_id="HOZ92764.1"
                     /translation="MLADFVVSIDHGQVVVHGEGEPGAGLLWTDEHVAQGFAWSEKLL
                     TLGVPDHDGECRIQVELVPEATVSAQALWAVQMPLEVTQPLHVGALFERHRVVVPNGR
                     YALLYQALPGTQGEAYVLRLSLAATPQPAFRILRTGGDVTADAVLRRDAQLLG"
     gene            complement(11790..12059)
                     /locus_tag="PK242_01685"
     CDS             complement(11790..12059)
                     /locus_tag="PK242_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92765.1"
                     /translation="MPLPTRRLAAIALLATLIASPTLALAKKEGAGKTPRSGAGSRGV
                     RLKPSPTATYASGESPAERQRNEDARLRRECRGRPNAGACLGYTR"
     gene            12144..13319
                     /locus_tag="PK242_01690"
     CDS             12144..13319
                     /locus_tag="PK242_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOZ92766.1"
                     /translation="MRQAVLNLEESKIREVANAGMGRADVLPFWFGESDEVTPEAVRA
                     AGIASLQAGETFYSHNLGLPELREAVAAYASRLHGELAPQRLAITSGGVNALIVAMQA
                     LIDAGDEVLAVTPVWPNLTAQPRILGAQLRCVALQPDAEGAWQLDVPALLAAITPRTR
                     LLVLNSPNNPTGWTLTRAEQQAILAHCRQTGTWILSDEVYERLYFRDDTANGAAPSFL
                     DIAAPDDRLVVVQSFSKAFLMTGWRLGWLVMPPAMTPQVGKLLEFNTSCAPVFVQRAA
                     LVALAQTEAITPRIVAHLRHCRDTLVPLLQAVPGVQVGRAPGGMYAFLRLVGQNDSLQ
                     LAKRLVQEAGLGLAPGVAFAPQAEGWLRWCFASRDVARLRQGVDRLHHWLRTAAPAP"
     gene            13316..14332
                     /locus_tag="PK242_01695"
     CDS             13316..14332
                     /locus_tag="PK242_01695"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOZ92767.1"
                     /translation="MNPGSRPRRRVPALSGASLSRRLRLAQLDLFEKVLETGSILAAS
                     RELAITQPAVSKSLRELEQQLGGALFVRGKHGVTLTELGQLFARHARAMTSGLQDMAD
                     ELNAWHGGTAGRVVVGAMLSATVALLPEAISRLRDMAPDVLVTIRVGPNSVLFPALAR
                     GEIDLMVGYLPANASAATKDAAPGTRLVHVPLYDEGMSVVVGPDHPLAHRTDVRVAEL
                     HRFEWIVPTPDAVAYPAVRALFEREGLSLPRSRVESISILTNVGLLARRPMVALMPHS
                     VATRLASAGLVVVLPLPSLGSFSALGYTRRAEQGMNMALQHLIDALHAVGDALQRGAG
                     LDLS"
     gene            14476..15963
                     /locus_tag="PK242_01700"
     CDS             14476..15963
                     /locus_tag="PK242_01700"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HOZ92768.1"
                     /translation="MGRSFALPATLGSLRNYVDGEFVETGRTFDNVSPIDGSVLAKVH
                     EADAALVDRAVRAARRALDEGPWGRTRVEQRAEALHRVADIIARRFDEFLQAEVADTG
                     RPVEQARKLDVYRGIANFRSFADLGKTAHGEFYETHLPDGSELINYTKRKPLGVVASI
                     SPWNLPILSLTWKAAPALICGNTIVCKPSEETPSSAVLIAEAFHEAGVPPGVFNVVHG
                     FGPGSAGEALTTHPGIDAVTFTGESRTGAAIMQAVAPGVKEVSFELGGKNAAIVFADC
                     DFEPTVEGMARALFTNTGQVCLCPERVFVERSIFERFVAALKAKTESLKIGWPDEPDV
                     AMGSLISREHREKVLSYFRLAVEEGATVVTGGGVPHFGDARDNGAFVQPTIWTGLPDS
                     ARCVREEVFGPVCHLSPFDSEDEVLRRANDSDYGLAATVWTSNLSRAHRVAPQLHVGI
                     VWVNTWFSRDLRTPFGGTRLSGIGREGGRWSLDFYSETTNICIKV"
     gene            15996..17249
                     /gene="kynU"
                     /locus_tag="PK242_01705"
     CDS             15996..17249
                     /gene="kynU"
                     /locus_tag="PK242_01705"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HOZ92769.1"
                     /translation="MPITLQQCQTWDQADELAALRDEFDLPAGTIYLDGNSLGAMPRN
                     ALARAQDIIARQWGVDLINSWNKAGWWQLPTRLGDQLAPLVGAGAGEIVVTDTTSLNL
                     YKVLANAVRIQQRRQPERKVILAERDAFPTDLYMVQGLLGFMDRGYTLELIDRPEDLP
                     SRLGEQTAVVLLSHVNYRTGYQWDLSAASQQAHAAGALIVWDLCHSIGAVPIDLEAAD
                     ADFAVGCTYKYLNGGPGSPAMLWAKPRHRADFWQPLSGWWGHARPFDMAVDYAPDRGI
                     RGFLCGTQPIVSLGLVACGLEIFAKTDMHKLRRKSLALTDLFIALVEQECAGQGLTLV
                     TPREHAQRGSHVSFRHPEGYAVVQALIARGVIGDYREPEVARFGVTPLYLRFVDIWDA
                     VQHLKAVLTQREWDQPRYRQRGAVT"
     gene            17275..17730
                     /locus_tag="PK242_01710"
     CDS             17275..17730
                     /locus_tag="PK242_01710"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOZ92770.1"
                     /translation="MTATFNRSTQASVVAGKATPRGRFPHIKRAGDFLFVSGTSSRRP
                     DNSFAGVQVDALGVTNLDIRAQTRAVIENIRDILLSQGAQLSDLVEAQVFLVNMNDFG
                     GFNETWAEFFDYEGPTRTTVAVHQLPHPHLLIEIKATAYHPRPGTGQPR"
     gene            17742..18305
                     /gene="nbaC"
                     /locus_tag="PK242_01715"
     CDS             17742..18305
                     /gene="nbaC"
                     /locus_tag="PK242_01715"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HOZ92771.1"
                     /translation="MQPKYGLPKNLMDWVGAHSDRLRPPVCNAALFPDGNYIINMVGG
                     GNTRTDFHDNPTEEIFYQIRGTAYLNIWDRGKFDRIDLKEGDVWLMEPHLQHSPQRPD
                     PHGLCFLVELKRPQGAIDTLNWYCPRCAALVWGASMELKDLVADLPATYQKFYALSDA
                     ERTCPHCGTVHPGKDYAAWHAIREAHA"
     gene            18302..19321
                     /locus_tag="PK242_01720"
     CDS             18302..19321
                     /locus_tag="PK242_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOZ92772.1"
                     /translation="MSGALSMIDIHAHFLPQIGQQEAAAVERERAPWLRIDAGGETGQ
                     IMVGEREFRPVYRALWDADFRVAELDAQGIALQLVCATPIMFGYAWEAGRAADWAAQM
                     NDKALSFCARHPTRLKALAQVPLQDTARACAEASRAMAAGCVGVQIGNHVGDKDLDHP
                     DLVDFLIHCADHRIPVLVHPWDMMGGPGRMKQWMLPWLVSMAAETQLGMMSLILSGAL
                     ERIPTSLRICFAHGGGSFAYLLGRADNAWRQRDIVRKDCPRPPSEYARRLHTDAIVFD
                     PRALHLLIDVMGVDNVMLGSDYPFPLGEQQVGSLVRGVDFLSGSQRAQILSDNARRFF
                     ALPEG"
     gene            19370..20533
                     /locus_tag="PK242_01725"
     CDS             19370..20533
                     /locus_tag="PK242_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ92773.1"
                     /translation="MKSTVRNVIAAAALSMLASHALADVKIGFMATMSGSAAALGQDM
                     YDGFMLALEQNGGKLGGQKIDLIKEDDQLKPDVGVQIVQKFIEKDKVDIVAGVTFSNV
                     MMAIAKPLADADMTYVGSNAGPAPLAGKQCSKGFFFASWQNDQQAEVMGAFANDKGYK
                     KAYLLAPNYQSGRDQIAGFKRQYKGSVVGEVYTQLNQPDYSAELAQLQAASPDVVYVF
                     YPGGMGVNFIKQYRQAGLLGKVPLVSASTVDGTTLPALKEIALGAVTGTFWGPDFNNA
                     TSKAFVAAFEKKYNRIPSQYAAQAYDSALLIDSALKKTGGKVADKQALRTALRAADYA
                     SLRGPFKFDVNGFPIQDFHVFEVAKDSAGRVSLKTIATPLRAHRDAYVGECKL"
     gene            20787..21707
                     /locus_tag="PK242_01730"
     CDS             20787..21707
                     /locus_tag="PK242_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ92774.1"
                     /translation="MTPELLLIQTLNGLQFGVLLFLMAAGLTLVFGIMSFVNLAHGAL
                     YMFGAYFAAAAWNASGSFLLAVLGGFGGAFVLGALVDRVGLFALYPRDHLDHVLATFG
                     LVLFANELARMIWGASPVFMELPESLAASVPLLGINYPVYRMLIIVVGLAVAACLYLL
                     IERTRVGMLIRAGASNRAMVSALGVNIGWLNTFIFALGAGLAGLAGAMVGPIMSVQPG
                     MGDPILITTLVVIVIGGIGSIRGAFLGALVVGVVDTVGRTLVPALLRGALAPEMANTL
                     GPALSSLAVYVLMAVVLALKPDGLFPVRKR"
     gene            21821..22951
                     /locus_tag="PK242_01735"
     CDS             21821..22951
                     /locus_tag="PK242_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ92775.1"
                     /translation="MASATPCGRRPAASPRVDGRADHGAGGRAQRLFPGLKGRNMVSS
                     SVGVHAADRLAPTLLGLSKARWRTVLTVLLAAFFVLVPLYAWLTGDSYVLILMGRILV
                     FALAAVGLNLALGYGGMVSFGHAMYLGIGAYVVAIASHHGLDNGLVHLLLVAALTVAV
                     AIPVGLIALRTQGIAFIMITLAFAQMLFFVAVGLKQYGGDEGLSIVHLSRFGALTGNK
                     TALYLSMLLALGLALWLLRRVVASRFGLVLRATMINERRVRAVGTPPLGYRLAGYVAS
                     ALLCALAGYFLANLTSFASPAYMAWTVSGELIVMVLLGGMGTLVGPVVGALVFLLLEE
                     GLKALTDHWLVIMGPVIVVIVLFLKNGLWGIVSDVPAARGGH"
     gene            22953..23717
                     /locus_tag="PK242_01740"
     CDS             22953..23717
                     /locus_tag="PK242_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ92776.1"
                     /translation="MTQSQPLLSLRGLVKRYGGLLVTDHVDLDVLPGEVHAIIGPNGA
                     GKTTLIGQIVGEVASDEGSIRLQGQSIDGLSVPERARAGLGRSYQITSVIGAFTVLEN
                     VILAVQGCLGHAFHFWRPASETAELVERADEVIERVGLSAQRDVHAAALAYGQQRQLE
                     MAMALAMQPSVLLLDEPMAGMGAVESSQLVALLQRLKGQYGILLVEHDMGAVFALADR
                     ISVLFHGKVICCGDPATVKAHPLVREAYLGDEEIPA"
     gene            23720..24418
                     /locus_tag="PK242_01745"
     CDS             23720..24418
                     /locus_tag="PK242_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ92777.1"
                     /translation="MLEVEGLAAGYGQSQVLFDIGLAVAPGQCVTLLGRNGMGKTTTI
                     KTIMGLLRPWGGVVRWKGRAVQGRPPHAMARLGLGLVPEGRQVFPNLTVRENLVATGR
                     GGGSSHAWTLARVHALFPRLAERASNFGNQLSGGEQQMLAIGRALMTNPELLILDEAT
                     EGLSPLVRREIWLALRQIKAEGMAILVVDKNLAPLLELAERHHIVEKGQVAWSGSSEE
                     LRDQRSLMQRYLSA"
     gene            24712..25119
                     /gene="speD"
                     /locus_tag="PK242_01750"
     CDS             24712..25119
                     /gene="speD"
                     /locus_tag="PK242_01750"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOZ92778.1"
                     /translation="MVASGATLRSAHAVARMAPGLHLIGDLYGCRGDTRLMTDAATLE
                     AFCKQAVADAGLTTVGSLFHSFGEGEGVTGAVVLAESHLALHTWPEDNYVTLDVYVCS
                     YTNDNSAKAERLFDALMQAFQPADPHLHRVVRA"
     gene            25128..26087
                     /gene="speE"
                     /locus_tag="PK242_01755"
     CDS             25128..26087
                     /gene="speE"
                     /locus_tag="PK242_01755"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOZ92779.1"
                     /translation="MSASIGTGGQFAVEQLSPDFGFYLRTTGLLAERRSSMQHIEIVD
                     TPLFGRAMRIDGCFMTSERDEFFYHEPMVHLPAIAHGDPRQALVVGGGDGGAAHHLLR
                     YPNMARVVLAELDRDVIDMARAWLPAVHRGAWDDPRLELRLGDGRAFIESCESRFDQI
                     VLDLTDPFGPAVALYTCEFYRACRRALRPGGVLSLHVQSPIHRGATMARLLASLRSVF
                     PVVRPFLQYVPLYGTLWAMAMASDRADPLALTAAEVDARLARHGLNDLQLYSGDTHLA
                     LLSLPPFVRRLLAEPARPVVDGDSLDDPSLDPGAERTLRLVRG"
     gene            26191..26748
                     /locus_tag="PK242_01760"
     CDS             26191..26748
                     /locus_tag="PK242_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOZ92780.1"
                     /translation="MKKLLIAVALGLAGWSAGAVETGISAREAYEKVQSNAPGVLFVD
                     VRDPVEIMFIGFTDVVHTNIPFMMVDRSKWDEQRGIFRLYPNPDFVAQIKAELARRGM
                     GMDAEVITLCRSGSERGEPSARLLRENGLPNARYVINGFQGAALKDGPQAGLRIQNGW
                     QNSGLPWSAKMSPGKVYRVDRQPGN"
     gene            26884..27150
                     /gene="rpsO"
                     /locus_tag="PK242_01765"
     CDS             26884..27150
                     /gene="rpsO"
                     /locus_tag="PK242_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOZ92781.1"
                     /translation="MISKENKAAVVKDNARAANDTGSPEVQVALLTARINELTPHFKE
                     HAKDHHGRRGLLRMVSRRRKLLDYLKAKDADRYLALIAKLGLRK"
     gene            27366..29648
                     /gene="pnp"
                     /locus_tag="PK242_01770"
     CDS             27366..29648
                     /gene="pnp"
                     /locus_tag="PK242_01770"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOZ92782.1"
                     /translation="MSMFNKITKSFQWGDKTVVMETGEIARQATGAVLVDIDGTVILA
                     TVAASKSAKPGQDFFPLTVDYIEKTYAAGKIPGSFFKREAKPSELETLTSRLIDRPIR
                     PLFPEGFFNEVHVVIHTVSLNPEVDADIAAMIGVSAALSISGIPFNGPIGAARVGYIN
                     GEYVLNPGPTARKSSQLDLVVAGTEAAVLMVESEADQLSEDVMLGAVVFGHQQGQIAI
                     NAIHELVREAGKPLWADNGTWVPEAKDQAFIAQVNAAAEGPLRAAYQIRSKQARTQAC
                     REAYAGVKAALTEQGVAFDPVKLDNLLFEIEARIVRGQILAGEPRIDGRDTRTVRPIE
                     IRNSVLPRTHGSALFTRGETQALVVTTLGTERDAQRIDALAGEFEDRFLFHYNMPPFA
                     TGEVGRMGSTKRREVGHGRLAKRALIPLLPAKDEFPYTIRVVSEITESNGSSSMASVC
                     GGCLSLMDAGVPMKAHVAGIAMGLIKEDNKFAVLTDILGDEDHLGDMDFKVAGTTAGI
                     TALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQEAMGQARTEISSYAPKLYTMKI
                     NPEKIRDVIGKGGATIRALTEETGTTIDIAEDGTITIASTDAAKADHARRRIEEITAE
                     AEVGKVYEGPVTKLLEFGALVNILPGKDGLLHISQIAHERVEKVSDYLQEGQVVKVKV
                     LETDDKGRIKLSMKALLERPAHLEGSDERPPRGDRPPRGERGERRERAPREDRGEREE
                     RPAPAAGAPEAAPELPSPQI"
     gene            29709..30728
                     /locus_tag="PK242_01775"
     CDS             29709..30728
                     /locus_tag="PK242_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOZ92783.1"
                     /translation="MKCIEIREPGEPAVLVPAERPLPQAGPGELRIRVSASGVNRPDV
                     LQRAGHYPAPPGASDLPGLEVAGVIESGDAAALSAAGLQLGQRVCALVAGGGYAEVCV
                     APVAQCLPVPAGCSDVEAAALPETFFTVWSNVFDRGRLQAGETLLVQGGTSGIGVTAI
                     QLAKAAGATVIVTAGSDDKCAAALRLGADHAINHRTQDFAQQALQLTGGRGVDVILDM
                     VAGDYVQREVECLAEDGRLVIIAVQGGTRSQFNAGLVLRRRLTITGSTLRPRPVAFKA
                     AIAQALRQRVWPWLEQGRVRPVIHQVWPALEAGDGLPSGAARAHALMESGQLIGKLVL
                     TWSQP"
     gene            30725..31477
                     /gene="tpiA"
                     /locus_tag="PK242_01780"
     CDS             30725..31477
                     /gene="tpiA"
                     /locus_tag="PK242_01780"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOZ92784.1"
                     /translation="MSERKKLVAGNWKMNGSLAANQALVQALRQGVGQPACDVAVCPP
                     SVYLGQLQQLLAGQTAIALGAQDLSQHEGGAFTGDVSATMLKDFGVRYAIVGHSERRQ
                     HQGETDLQVALKVRRALAAGITPIVCVGETLREREEGLTEFIVKRQLSAVLHLNGHAI
                     NETVVAYEPVWAIGTGQTATPAQAQSVHAVLRAQLRAAGAQAEHVRILYGGSMNAGNA
                     AELLAQPDIDGGLIGGAALKAPDFLTIVAAAA"
     gene            31518..31961
                     /gene="secG"
                     /locus_tag="PK242_01785"
     CDS             31518..31961
                     /gene="secG"
                     /locus_tag="PK242_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOZ92785.1"
                     /translation="MNVILNLILVVQLLSALAMIGLILVQHGKGADMGTAFGSGASGS
                     LFGATGGANFLSRTTAVLATVFFVCTLALAYFGFSVRSAPADSGSVLDRAAVTAPAAG
                     ASAAAPATAADAIPSGGTPAPATEPSRPATPAAPAASGAGQIPGQ"
     gene            32088..32172
                     /locus_tag="PK242_01790"
     tRNA            32088..32172
                     /locus_tag="PK242_01790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32122..32124,aa:Leu,seq:gag)
     gene            32231..32590
                     /locus_tag="PK242_01795"
     CDS             32231..32590
                     /locus_tag="PK242_01795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOZ92786.1"
                     /translation="MNLAQYLPVLLFILVGLAVGALLLGMGNFLKPHKADAAKNSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVSLREVGLVGFVAVLIFLAI
                     LVVGFVYEWKKGALDWE"
     gene            32630..33109
                     /locus_tag="PK242_01800"
     CDS             32630..33109
                     /locus_tag="PK242_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOZ92787.1"
                     /translation="MNSEFQQKGFLLTGVDAAVNWAKTGSIWPVTFGLACCAVEMMHA
                     AASRYDIARFGAEVFRASPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGCDRVVPVDVYVPGCPPTAEALLYGIIQLQQKVRRTHTIARV"
     gene            33112..>33712
                     /locus_tag="PK242_01805"
     CDS             33112..>33712
                     /locus_tag="PK242_01805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOZ92788.1"
                     /translation="MTKHAIAPEVLNDTVASALGELVQHIDVARGEVTVSVSAGEYHQ
                     VMRLLRDAAGCRFEQLIDLCGVDYSTYGDGRWEGPRFAVVVHLLSVSLNQRLRVRVFC
                     TDDEFPVIRSVTDLWSGADWFEREAFDLYGIVFEGHADLRRILTDYGFIGHPFRKDFP
                     LTGHVEMRYDAERQRVIYEPVTIEPREITPRVIREDNYGG"
BASE COUNT         5382 a        11297 c        11778 g         5255 t
ORIGIN      
        1 tggtggcggg cgcggcgccg ccggcctcga tgatcgaggg catggagcgc ctgggctttg
       61 atctgaccca cgtctacggc ctgaccgagg tttatggccc ggccaccgtc tgcgccaagc
      121 acgagtcctg ggagcagctg cccatcggcg agcgtgcggc gctcaacgcc cgccagggcg
      181 tgcgctacca cctgcagcgc gacgcgcgcg tgctcgaccc cgagaccctg cagccggtgc
      241 cgatggacgg cgaaaccatg ggcgaaatca tgttccgcgg caacatcgcc atgaagggct
      301 acctgaagaa ccccggtgcc accgaggaag ccttccgtgg cggctggttc cacagcggcg
      361 acctggcggt gcagtacccc gacggctaca tcaagatcaa ggaccgcagc aaggacatca
      421 tcatttcggg cggcgagaac atctcttcca tcgaggtcga agacgtgctg taccgccacc
      481 ccgccgtgct ggccgcggcg gtggtcgcca agcccgaccc gaagtggggc gaaacgcctt
      541 gcgcctttct tgagctcaaa cccggtgccg aggtcagcga agccgacatc gtggcgcact
      601 gcaagcagca cctggccggc ttcaaggtgc cgcgcgccat cgtgttcggc gagctgccca
      661 agaccagcac cggcaagatc cagaagttcg agctgcgcaa acaggcgggt tcggccgcgg
      721 cgatcgatgt ctgaatttgc tatcaggaat atagctgttg gcgcagtttg aacaagcgct
      781 gagcggcgat tcgatgtctg atcgccaaaa gggggtgtgg ggcacccgcc ggttcagtcc
      841 tgtggcgccg gtgcctgcgg ccgcagtgcc tccggcacgt cggtgcggcc ggcgacgcgg
      901 tggcgaaaca gcgccgcgcg gctcagcagc acggtggtga tcggcaccgt gatggacaac
      961 aggatgatga tgatccacac gtgcagctgc ggcccctgct gctggcccga aaagaacagg
     1021 atcgaagcca gggtcacgca ccaggaggag cccacgtaca ccagggccgg tgggtgcatg
     1081 cgctggtaga agctggacag ccgcaccagg ccccaggcgg ccgccagagc gaacacgccg
     1141 ctggccagca gcaggccggc gatcagccac tgcagccatt gcggcagggc gtcggcgctc
     1201 attcgatcac ctcgccgcgc agcaggaact tggccatcgc cgccgaaccg acgaaaccga
     1261 acagggcgat cagcagggcg gcctcgaaat acaggtcgct ggcgtggcgg atgcccagca
     1321 ccagcatgat cagcatggcg atgacgtaga tcatgtccag cgccagcacg cggtcctgtg
     1381 ccgtcgggcc gcgcagcacg cgccacaggg cgatcagcat ggcgacggcg taggcaccca
     1441 gcgtcagggc cagcgcgccg gagagcaggg ggctcatgac tcgaagatct ccttcagcgg
     1501 ttgttcgtag cgtcgtttga agaaggcgac gaattcggcc tcatcgggca catgccacac
     1561 gtgcagcagc atggcgctgc agtcgcgcgc cagttcgcac cagacggtgc cgggcaccac
     1621 ggtggtgatc atggtgagcg aggccagcgc ggtagggtcg cgcagcgtca gcggaatcac
     1681 gacgaagcgg ctgtggacgg gtcggctgcg gttgcgcacc agggtccaga acacgtccag
     1741 gctcgacacc aacacgtcgt gacccacggt caggatcagt cgcagcacct tcagcgggtg
     1801 ccgtatccgc gggcgcgccg ggcgcagcgg tgccagcagc gccggcgccg ccacgccgaa
     1861 cagcagcgcc agcggcagct gtcccaggct gaacggccgg ttcagcacca gccacagcag
     1921 gatcagcgcc gccgtcagca gcggtgcggg cagccagcgt ctcatcggcg cagtcctccc
     1981 tggtcgctgg cgtggggcga aggctgcggc cgcgccgcca tcacgctgcc gatgtagtcg
     2041 cgtgggtcgt gcaggtggcg cgcggtggcg ttggcgtagc gcagcacggc gtcggcctgc
     2101 cacaccagca cgccgatggg cagcagcagc agggcgatcg gcagggtttc gatgatgcgc
     2161 aggcgcggcg gtggccggcc ggcgggcgcc cagaagcagc gtatgccggc gcgcgacatg
     2221 gcgatgatgg tcagcaaccc gcccgtcacc agcagcgcca gcagcagcca ggcggccggc
     2281 gaaacgggtg cctgcacgcc gaagccggcc gggttgagca gcgccgacag catcaccacc
     2341 ttgccgacga agccggccat gggcggcagg ccggcaatgg tgagcgcgca gacgatgaag
     2401 gccagcccca ggaaggccat ggcggccgga atggcgcggc cgatcagcgc ctcttcgtcg
     2461 tcgtcgaggt tgacgccgtg cgtgggcacc agatcgaccg ggaaggcaaa cggttcgtcg
     2521 tccgggtcgg actcgcccac gtacaccggc tgctcttcga cgtcgcgtgc gcggtcgatc
     2581 agctccacca gcagaaagaa ggcgcccacc gccagcgtgg agcccagcgc gtagtacagc
     2641 gcgccgcccg tcaccgccgg ccgaccgaag ccgattgcgg ccagcagcgt gcccgacgac
     2701 acgatggcac agaacgccgc catgcgcccc agctgctgca ccgcgatcac gccgatcacg
     2761 ccgaagccca gggtgagcat gcccagcccg atcagcaccg ggccgccgaa caacgcggag
     2821 atgccggcgt cctgcgggaa cagcagcgtc cacaggcgca ggatggcgta cacgcccagc
     2881 ttggtcatga ccgcaaacag cgccgccacc ggcgccgccg cgctggcata ggcggccggc
     2941 agccaggcat tgaacggcca ggcggcggcc ttgaccagga aggcgatcgc caggatggcg
     3001 gcgccggcgt gcagcagacc gcgatcgtcc tcggcgatgg tcggcacgat gatggccatg
     3061 tcggccatgt tgagcgtgcc ggtgatgccg tacagcatgg ccaccccgat caggaacagg
     3121 ctggacgcca tcaggttgat ggcgatgtag tgcaggccgg cgcgcacgcg cggccaaccg
     3181 gacccatgca gcagcaggcc gtaggaggcc gtcagcatga tctcgaagaa cacgaacagg
     3241 ttgaacaggt cggccgtcag gaaggcgccg gccaggccca tcagctgcaa ctggtacagc
     3301 gggtgaaagt gcaccccggc ccggtgccag cgcgcgctgg cgtagagcac gccggccagc
     3361 ccgagcaggc tgcccagcag cagcatcatg gccgacagcc ggtcggccac cagcacgatg
     3421 ccatagggca cgggccagtt gccaggcagg tagacgccga tcgactgcgc cgcgccactg
     3481 cgctgcacct gcagcaccag cgcggcagcc accagcacgc cggcggcgct ggccagcacg
     3541 ttgagcgcgg ccttcagcgg ccggcgcgtg tcgcccagca gcagcatcag cgcggccgtc
     3601 agcatgggca acaggatggg cgccaccacc aggtgcggca tcagggactc gatccacgcg
     3661 gacaggttca ccggggtggc cctccggcgt cgtcgcgacg caggggcagg gccgtgttgt
     3721 cgtcgacgtt gtcgccgtcc acgtggtcgt tgtcggccag gccgcgcgcg gccatcagca
     3781 ccaccagaaa cagggccgag gtggcaaagc cgatcacgat ggcggtcagc accagcgcct
     3841 gcggcatcgg gtcggtgtag tgctccaggc tgggagtcag gccgggctgc acgatggggg
     3901 gcttgtcctc gaacaggctg cccatggcaa agatgaacag gttgaccgcg tacgacagca
     3961 gcgacaaccc catcagcacc tggaaggtgc gcggacgcag caccagccac acaccgcaac
     4021 cgcccagcac gccgatggcc agggccagga tcacttccat ctcagccgac ctccggcagg
     4081 cggtgggcgg gagcgtcggt ggccatcgtc tccggtgcct ggcgatacgc gcgcaccgac
     4141 tggtgcgcca gcgccgtcag gatcagcagc gtggcgccca ccaccacggc gaataccccc
     4201 aggtcgaaaa acagcgcgct gggcaggtgg acatcgccca gcagcggcag gtgcaggtgc
     4261 gcggtgtgcg tggtcagcag cggaaagccc agtggcagcg agcccagacc ggtgagcgcg
     4321 gccagcagca ggccgagggc gatccagcgg atcaccttca gttgcagatg cgcttccacc
     4381 cacagcgtgc cggccacgat gtattgggcg atgaaggcga tcgccaccac cagaccggcg
     4441 acgaagccac ccccgggctg gttgtgcccg cgcatgaaca ggtgcatggc gatcaccgcc
     4501 gccaccggca gcagcaggcg caccagcacc gccggcacca gcatgtagcc cagcgccgtg
     4561 tcctgtgccg tgcgcggatt gatcaggtcg ctcaccagat cggaggcgat ggcgcgttgc
     4621 tgcggcggca gcgtggccat ctcgcgcggc ggacgaaagc gccgcagcag ggcgtacacg
     4681 gtgagcgcca cgatgcccag cacggtgatt tcgcccaggg tgtcgaaggc acggaagtcc
     4741 accagcatca cgttgaccac gttgttgccg ccgccttcgg gcagcgcgcg gtcgatgaag
     4801 aagggcgcga tgctctgcgg cgccgggcgc gtcatcatgg cgtaggccag cgccgccatg
     4861 ccggtgccgg caaccccggc caccagcagg tcgcgcaggc gccgtgcgcg cacccgcggg
     4921 tgcaggcgct ggcctggctg cggcatgcgc ttgggcagcc agcgcagacc cagcaaaaac
     4981 aggatggtgg tgaccacctc caccgtcagc tgggtcagcg ccaggtcggg cgccgacagc
     5041 cagacgaagc tcaggctcac caccaggccc actgcgccca gcatgatcag tgccgccagc
     5101 cggtggaact tggcctggtg agcggtgccc agggcggcca gcccaccgag cacccacagc
     5161 agggcaaacg ccgcgctgcc gggcacgcgc tcgcgttcgc cccagctgat gccggtcggc
     5221 cacagcgccg ccgcgcccag cacgatggcc gccagcacca ccaccagcaa ctgtggctgc
     5281 aggcgtggcg tgctcagacg cctgagcagg ccgcggctgg cgcggtccag acccagcagc
     5341 aggcgctgga acagccgctg cccgtccagc cggcgcggca gcgccagcag ccgcaggtcg
     5401 cccgcggcca ggcgctgccg gccccacagg aacatggcca cgccgccgac cagggcgatc
     5461 aggctcatca gcagcggctt gttgatccca tgccagatcg ccaggctgta ggccggcatc
     5521 gccccgccca ccaccgggcg ggccgccagc tccagcgccg cgccgatggc ccattgcggc
     5581 agcacgccca ccagcaggca caccagcacc agcacctcga ccggtgcgcg catccagcgc
     5641 gacggttcgt ggggcgtcag cggcaggtcc ctggccggcg ggccgaggaa cacgccgacc
     5701 gcgaaacgca gcgaatacac cacggcaaaa acgccggcca gcgtggcgac gacgggcaag
     5761 ccgatgtcga gcgtcgggtt gcggctcagg tgcacggttt cggcaaagaa catttccttg
     5821 gacagaaagc cgttcaagag cggcacgccg gccatggcgg cgctggccac gaacgccagc
     5881 gtgccggtga tcggcatggc ctgccacagg ccggacaggc gccgcagatc gcgggtgccg
     5941 gtttcgtggt cgatgatgcc gacggccatg aacagcgagg ccttgaaggt ggcgtggttc
     6001 atgatgtgga acaccgccgc caccgccgcc agctcgctgc ccaggcccag cagcagggtg
     6061 atcaggccca ggtggctgat ggtcgagtag gccagcagcc ccttgaggtc gttctggaac
     6121 atcgccagca gggcgcccag cagcagcgtc aacaggccgg cgctggcgac gatccagaac
     6181 caggcttcgc tgcccgccag cgccggccac agccgggcca gcaggaacac cccggccttg
     6241 accatggtgg ccgaatgcag gtaggccgac accggcgtgg gcgccgccat ggcgtgcggc
     6301 agccagaagt ggaacggaaa ctgtgcgctc ttggtcagcg cccccagcag caccagcagc
     6361 atcatgggga cgtacaggcg gtgcgcccgg atctgttcgc ccgcacccag gatgtcgtcc
     6421 agccggtagc tgcccgcgat gtgacccagc agcagcacac cggccagcag cgccaggccg
     6481 ccggcgccgg tcacggtgag cgccatgcgt gcgccgcggc gggcgtcctt gcggtggtgc
     6541 cagtagccga tcagcaggaa ggaaaacagg ctggtcagct cccagaagaa cgccagttgc
     6601 accacattgc ccgacagcac cacgcccagc atggcgccca tgaaggcgag gaagaacgaa
     6661 aaaaagcgcg gcaccgggtc ttcgcggctc atgtagtagc gcgcgtacag tgccaccagg
     6721 gtgccgatgc cggtcaccag cagggcgaac atccaggcaa agccgtccat gcgcagcgag
     6781 aagtcgatgc cggccgaggg cagccagcgc agctcctggc gcagtacggc gccgccctgc
     6841 agcgccgggt agcgcgtggc cacccacacc agcgccgcgg cgcccaccag ggccgcccag
     6901 cccgccgccg cgttgcgcgc atgcgtgggc agcagcgccg ccaccaggct gccggcgaag
     6961 gggagcaaca acaggtagat gaggtccatc gggtggctgg ggcgatcggc ggggcgggcg
     7021 cggcggcagg tgttcccggg tcgccgctgc agcaccgaat ataccagtgc cattcatggc
     7081 ttgggcgctt gtgggacaag cgccagaagc tatcaaaata ggagttttgt ggaagctgtc
     7141 gtgcggcctc acaatgccgg catgcggatg ctgctggtgg aagacgacgc catgatcggc
     7201 gaggtggtgc tggacgcgct gcgcgccgag caccacgcgg tggactgggt gcgtgacggc
     7261 gccatggcgc tgacggcgct ggccaccgcc ccctacgatc tgctgctgct cgacctgggc
     7321 ctgccgcgcc aggacggtct gagcgtgctg cggcagctgc gcgccgacca ccagcggctg
     7381 ccggtgctga tcgccaccgc gcgcgacgcc gtggccgacc gcgtggccgg gctggacgcc
     7441 ggcgccgacg actacatcgt caagccctac gacctggatg aactgctggc gcgcgtgcgt
     7501 gcgctgctgc gccgcgccga cgggcgcgcc gagccggcct acgaatgggg cgaggtgcgc
     7561 atcgtcccca gcacgcgcga agtcacgctg cgcggccagc cggtgcagct gtcggcgcgc
     7621 gaatgggcgg tgctggaacc cctgctggca cgcccgggcc gggtgctgtc gcgccagcag
     7681 ctggaagaca agctctacgc ctggcgcgac gaggtcagca gcaacgcggt ggaggtctac
     7741 atccacggcg tgcgcaagaa gctgggcgcc gggctgatcg agaacgtgcg cggtctgggc
     7801 tacctggtgc cggtggcgcc gcctgagtcg ggttcatgag tgctcctgga atggaagctg
     7861 ccagcgtaag tccatcaagc gctgagagcc aaaaagactt gaagacgccg gccccttcgc
     7921 tgcgcgcccg cctgacggcg ctgctgctgc tggcggtgct gctgacggcg gcgctgcagg
     7981 cggccatcac ctggcgcacg gcgctggccg agaccgaggc ggtgttcgat gcgcagatgg
     8041 agcgcatcgc gctgtcgctc accggcggcc tggccgccag cgtgctgggc gacgacgcgc
     8101 tggcggccac gcgcggcgcc gaggagctga tcatccaggt ctggcgcgcc gacggcatca
     8161 tgctgtaccg ctcgccctcg gcgcggctgc tgccgccgca gacggtgatc ggcttttccc
     8221 acctgcgcgc cggcgacgag gactggcgcg tgtacgcgct ggagacaccc agccaggtga
     8281 tccagatcgc gcaggccgag cgcgcgcgcc ggcgcatcgc cggccagttg gcgctgcgcg
     8341 cggtgctgcc ggtggcgctg ctggcgccgc tgctgatgct gatcgtgtgg tgggtggtgg
     8401 ggcgcgcgct ggcgccgatc gagcgcctgc gccgccagtt ggccgcgcgt ggcgccgccg
     8461 acctggcacc gctgccgacc gacggtctgc cggccgaagt gttgccgctg gtgaccggga
     8521 tgaacgggct gctggcgcgc ctgtcggccg cctggcagca actggccgac ttcaccgccg
     8581 acgccgcgca cgagctgcgc tcaccgctgg ccgcgctgcg tctgcaggtg cagagcctgc
     8641 agcgcgccga caccgagcag gcgcgcgccg tggccaccgg gcggctgctg gccggggtcg
     8701 atcgcgccac gcgcctggtc gagcagttgc tggcgctggc gcgccacgac ggcgatgcgg
     8761 acgcggtgcg cgagccggtc gatctgcccg tgctggcgcg ccaggcgctg caggaggccg
     8821 acgccgaggc gcaggcgcgc ggcatcgccc tgcggctgga aggtgatgcg tcgctgtggc
     8881 tggacgcccg gccgcaggcg ctggcggtgc tgctgcgcaa cctgatcgac aacgccttgc
     8941 gccacacgcc ggcgggcggg caggtgtgcg tggcggtgca gcgtcggccc gacggcgtgg
     9001 ccgagctggc ggtggaagac agcggccccg gcatcccagc ggccgagcgc cagcgcgtgc
     9061 tcgaccgctt ctaccgtgtg ccgggcgccg ccggccacgg cagcggcctg ggcctggcga
     9121 tcgtgcaggc ggtggcacgt cggcatggcg cgcaggtggt ggtgcaagcc tcgccgcgcc
     9181 tgggcggggc gcggctggcg gtgatctggc cggccgccgc cgggcctgag gactgatggt
     9241 gttttaggca tccagagcac gccagacaag cgccaaaagc tattgaaatc atagtattca
     9301 gcagccctgg cgtggtctta agcctggcct aattcccatc catcacagtg ccgccacggc
     9361 tccgctgacg gagcgtcttg ctgaaaggaa ctgtgatgaa ccgtttgacc ttgaaatcct
     9421 cacgtctgtt tgccgccctg ctggcggcgg gggtgatcgg cggcgccagc gtggcggcgg
     9481 tgcaggagct gacgcccgcc caggcgcagc cgagcgcggc cgtgcaggcg gtggcgccgg
     9541 cggccgtggc gacacccgac ttcgccgcca ttgccgcgcg ctacggcggc gccgtcgtca
     9601 acatcagcac ccgcggcgtg cagccggtcg ccgacgaggg cgccgacagc ggcccgggcg
     9661 ctcggatcga cccggggctg caggactttc tgcgccgctt cggcttgccg cccggcgcgt
     9721 tccagttcgc cccgcccggc cccggcgggc ccggcgcggt gccgatgcat ggtcagggct
     9781 cgggcttcat cgtcagcccg gacggcgtga tcctgaccaa cgcccacgtg gtgcgcaacg
     9841 ccaggaccgt caacgtcaag ctgaccgacc ggcgcgagtt ccaggccaag gtgctggggc
     9901 gcgacgacaa gaccgacgtg gcggtgctga agatcgatgc caccgggctg cccaccgtgc
     9961 cgctgggcag cagccaggcg ctgcgccccg gcgactgggt gctggcgatc ggctcgccgt
    10021 tcggcttcga gaacaccgtg acggtgggcg tggtcagtgc caagggccgc tcgctgccgg
    10081 acgacagcgc ggtgcccttc atccagaccg acgtggcggt caaccccggc aactcgggcg
    10141 ggccgctgtt caatgcgcgc ggcgaggtgg tgggcatcaa ctcgcagatc tacagccgct
    10201 cgggcggcta ccagggggtg tcgttcgcca tcccgatcga gctggccagg cacgtcgagc
    10261 agcagctcgt cgcgcacggc aaggtcgagc acgcacgcct gggcgtgacc atccagtcgg
    10321 tcaaccaggc cctggccgac tcgttcaagc tgcccaaacc cgagggcgcg ctggtggcgc
    10381 aggtcgagcc tggcagcgcg gccgagcgcg ccggcctgaa ggcgggcgac gtgatcctgc
    10441 gcgccaatgg ccaggcgatc gtcgattcgg gcgacctgcc cgcccgcatc gcgctggccc
    10501 ggcccggcga caagctggcg ctggaggtct ggcgccaggg ccggcccgag cagctgagcg
    10561 ccacgctgca gggcgcgcgg cctgccgaca ccgcgctggc gcagggcagc gcggccagcc
    10621 acggcaagct ggggctgtca ctgcggccgc tgcaagccga cgagaagcgc cagaccaggc
    10681 tggacgaggg tctggtggtc gagcaggcca gcggcgccgc cgcgctggcc ggggtgcagc
    10741 cgggcgacgt cgtgctgtcg atcaacggcc agccggccag cagcgtcgag aagctgcgcg
    10801 ccgtgctggc caaggcggac aagtcggtgg cgctgctgat cgagcgtgac ggccagcaac
    10861 tgttcgtgcc ggtgccgctg gcttgaaacc tgcccggctt ctgaatgacc cacgacctcg
    10921 cggcgcttcg atcaccgcag gggaaggggt catcaatatc aaatcgggct ctggcgcttg
    10981 ctgggcaagc gccggcagct atcgttggtg aagtttccgg gagggtgcac gatcggtgcc
    11041 acacggtgcc ccggccaggt cccgatcggg tggggctggc cgcgctgctg cctgcgggca
    11101 cgcgttggcg gcggcgtcgc gccttgccgg gcgcgccgat cgcgttgcgg gcaggtgcgc
    11161 cctgcggccg cttgcaggct cagcccagca actgcgcgtc gcggcgcaac acggcatcgg
    11221 cggtcacgtc accgcctgtg cgcaggatgc ggaacgcggg ctgcggcgtg gccgccaagc
    11281 tcagccgcag cacataggcc tcaccctgcg tgccgggcag cgcctggtag agcagggcgt
    11341 agcgaccgtt cggcacgacg acgcggtgac gctcaaaaag cgccccgacg tgcagcggct
    11401 gggtgacctc cagcggcatc tgcaccgccc acagcgcctg tgcgctgact gtggcctcgg
    11461 gcaccagttc gacctggatg cggcactcgc cgtcgtggtc cggcacgccc agagtcagca
    11521 gcttttcgct ccaggcaaaa ccctgcgcca catgctcgtc ggtccacagc aggccggcgc
    11581 ccggctcgcc ttcgccgtgg accaccacct ggccatgatc gatgctcacc acgaaatccg
    11641 ccaacatcgt tgcggtgtcc tggtcggtgt ggggtagggg gcggtgtggg ggcatcggtg
    11701 ttcggatgcc gatgtcagat aaaaaatggc tccggcgctt gctgggcaag cgccagcagc
    11761 tatgcattca ggagttatca gggccgcttt cagcgcgtgt aacccaggca ggcgccggcg
    11821 ttggggcggc cgcggcattc gcggcgcagg cgcgcgtcct cgttgcgctg gcgctcggcc
    11881 ggcgattcgc cgctggcata ggtggcggtg ggcgaaggct tcaggcgcac gccgcgtgag
    11941 ccggcgccgc tgcgcggtgt cttgcccgcg ccttctttct tggccagggc cagcgtgggc
    12001 gaggcgatca gcgtggcgag cagggcgatg gcggccaggc ggcgcgtggg caggggcatg
    12061 gtggtctttc cgctggtgca gggccgcgct cgcacgggat atgcgatgct cgcgccctgc
    12121 cgaattaaat gagtccgtca ccgatgcgtc aagccgtcct gaacctcgaa gaatccaaga
    12181 tccgcgaagt cgccaacgcc ggcatgggcc gcgccgacgt gctgccgttc tggttcggcg
    12241 agagcgacga ggtcacgccc gaggcggtgc gcgccgccgg catcgcctcg ctgcaggcgg
    12301 gcgagacctt ctattcccac aacctgggcc tgcccgagct gcgcgaggcg gtggcggcct
    12361 acgccagccg cctgcacggc gagctggcgc cgcagcggct ggccatcacc tcgggcgggg
    12421 tgaacgcgct gatcgtcgcc atgcaggcgc tgatcgacgc cggcgacgag gtgctggccg
    12481 tcacccccgt gtggcccaac ctgacggcgc agccgcgcat cctgggtgcc cagctgcgtt
    12541 gtgtggcgct gcaacccgac gccgaaggcg cctggcagct cgacgtgccg gcattgctgg
    12601 ccgccatcac gccgcgcacg cggctgctgg tgctgaactc gcccaacaac ccgaccggct
    12661 ggacgctgac gcgggccgag cagcaggcga tcctggcgca ttgccgccag accggcacct
    12721 ggatcctgtc ggacgaggtc tacgagcgtc tgtacttccg cgacgacacc gccaacggcg
    12781 cggcgccgag ctttctcgac atcgccgcgc cggacgaccg gctggtggtg gtgcagagct
    12841 tttccaaggc ctttctgatg accggctggc gcctgggctg gctggtgatg ccgccggcca
    12901 tgacgccgca ggtcggcaag ctgctggagt tcaacacctc ctgcgcgcca gtgttcgtgc
    12961 agcgcgccgc cctggtggcg ctggcgcaga ccgaggccat cacgccgcgc atcgtggcgc
    13021 acctgcggca ctgtcgcgac accctggtgc cgctgctgca ggccgtgccc ggggtgcagg
    13081 tcggtcgcgc gcccggcggc atgtacgcct ttctgcgcct tgtcggccag aacgattcgt
    13141 tgcagctggc caagcgcctg gtgcaggaag ccgggctggg gctggcgccg ggcgtggcgt
    13201 ttgcgccgca ggccgagggc tggctgcgct ggtgctttgc ctcgcgcgac gtggcgcggc
    13261 tgcggcaggg cgtggatcga ctgcaccact ggctgcggac cgccgcgccc gccccatgaa
    13321 cccggggtcg cgcccgcgcc ggcgtgtgcc agcgctgtcc ggtgcgtcgt tgtcgcgacg
    13381 gctgcgtctg gcgcagctcg acctgttcga aaaggtgctg gaaaccggct ccattctggc
    13441 cgcttcgcgc gagctggcca tcacccagcc ggcggtcagc aagtcgctgc gcgagctgga
    13501 acagcagctc ggcggggccc tgttcgtgcg cggcaaacac ggcgtgacac tgacggagtt
    13561 gggccagctc ttcgcccgcc acgcgcgtgc catgacctcc ggtctgcaag acatggccga
    13621 cgaactcaac gcctggcacg ggggcaccgc tggacgcgtg gtggtggggg ccatgctgag
    13681 tgccacggtg gcgctgctgc cggaggcgat ttcgcgcttg cgcgacatgg caccggacgt
    13741 cctggtgacg attcgggtgg ggcccaattc ggtgttgttt ccggccttgg cgcgcggcga
    13801 gatcgatctc atggtggggt atctgccggc caatgcgtcg gccgccacaa aggatgccgc
    13861 tcccggcacg cgcctggtgc atgtgccgct gtacgacgaa ggcatgagcg tggtggtcgg
    13921 acccgaccac ccgctggcgc accggaccga cgtgcgcgtg gccgaactgc accggttcga
    13981 gtggatcgtg ccgacaccgg acgccgtggc ctatccggcc gtgcgcgcct tgttcgagcg
    14041 cgaagggctg agtctgccgc gcagccgcgt cgaatcgatc tccatcctga ccaacgtggg
    14101 tctgctcgcg cgtcgcccga tggtggcgct gatgccgcac tcggtggcta cgcgcttggc
    14161 cagtgccggt ctggtggtcg ttctgccgct gcccagcctg gggtcattca gcgcgctcgg
    14221 ctatacccgg cgcgccgagc agggaatgaa catggccctg cagcatctga tcgatgcctt
    14281 gcatgcggtc ggcgacgcct tgcagcgggg cgcaggtttg gatctgtcct gagcggcggg
    14341 ctcggcgaac atctatatcc gagagttata gatgagtcac ccgaactcat ttgccagccg
    14401 caggtggcct gcctagactg cgcgatatca gacgtcacgg ggcaggtgtg acccggacgg
    14461 cacgaggagc gcgtcttggg acgatctttc gcactgcccg ccacgctggg ctcgctgcgc
    14521 aactatgtgg atggcgaatt cgtagaaacc ggcaggacct tcgacaacgt cagcccgatc
    14581 gacggcagcg tgctggccaa ggtgcacgag gccgacgccg ccctggtcga ccgcgccgtg
    14641 cgcgccgcgc gccgcgcgct ggatgaaggc ccctggggcc gcaccagggt ggagcagcgc
    14701 gccgaggcgc tgcaccgcgt ggccgacatc attgcccggc gcttcgacga gtttctgcag
    14761 gccgaggtgg ccgacaccgg acgcccggtg gagcaggcgc gcaagctcga cgtctaccgc
    14821 ggcatcgcca acttccgcag ctttgccgac ctgggcaaga ccgcgcacgg cgagttctac
    14881 gaaacccacc tgcccgacgg cagcgagctg atcaactaca ccaagcgcaa accgctgggc
    14941 gtggttgcca gcatctcgcc ctggaacctg cccatcctct cgctcacctg gaaggcggcg
    15001 ccggcgctga tctgcggcaa caccatcgtg tgcaagccgt ccgaggaaac gccctcgtcg
    15061 gcggtgctga tcgccgaggc cttccacgag gctggcgtgc cgccgggcgt gttcaacgtg
    15121 gtgcatggct tcggccccgg ctcggccggc gaggcgctga ccacgcaccc gggcatcgat
    15181 gccgtcacct tcaccggcga atcgcgcacc ggcgcggcca tcatgcaggc cgtggcgccg
    15241 ggggtgaagg aagtctcgtt cgagctgggt ggcaagaacg ccgccatcgt gtttgccgac
    15301 tgcgatttcg aacccacggt ggagggcatg gcgcgcgcgc ttttcaccaa caccggccag
    15361 gtgtgtctgt gtcccgagcg ggtgttcgtc gagcgatcga tcttcgagcg cttcgtcgcc
    15421 gcgctcaagg ccaagaccga gtcgctcaag atcggttggc ccgacgagcc ggacgtggcc
    15481 atgggctcgc tgatctcgcg cgagcaccgc gagaaggtgc tgtcctactt caggctggcg
    15541 gtggaagagg gcgccaccgt ggtcaccggc ggcggcgtgc cgcacttcgg cgacgcgcgt
    15601 gacaacggcg ccttcgtgca gcccaccatc tggaccggcc tgcccgacag cgcgcgctgc
    15661 gtgcgcgagg aggtgttcgg cccggtctgc cacctgagcc ccttcgacag cgaggacgag
    15721 gtgctgcgcc gcgccaacga cagcgactac ggcctggccg ccaccgtctg gaccagcaac
    15781 ctgtcgcgcg cacaccgcgt ggcaccccag ctgcacgtgg gcatcgtctg ggtcaacacc
    15841 tggttctcgc gcgatctgcg cacgcccttt ggcggcacca ggctgtcggg catcgggcgc
    15901 gagggcgggc gctggtcgct cgacttctat tccgaaacca ccaacatctg catcaaggtc
    15961 tgatgcccgt cgccagcctt gcccaaggag ccgtcatgcc gatcaccctg caacagtgcc
    16021 agacctggga ccaggccgac gaactggccg ctttgagaga cgaattcgac ctgcccgcag
    16081 gcaccatcta cctcgacggc aattcgctgg gcgccatgcc ccgcaacgcg ctggcgcggg
    16141 cgcaggacat catcgcccgt cagtggggcg tcgatctgat caacagctgg aacaaggccg
    16201 gctggtggca gctgccgacc cggctcggtg accagctggc gccgctggtc ggggcgggcg
    16261 cgggcgagat cgtggtcacc gacaccacct cgctcaacct gtacaaggtg ctggccaacg
    16321 cggtgcgcat tcagcagcgt cggcagcccg agcgcaaagt gattcttgcc gagcgtgacg
    16381 cctttccgac cgacctgtac atggtgcagg gcctgctggg cttcatggac cgtggctaca
    16441 cgctggagct gatcgaccgc ccagaagacc tgccctcgcg gctgggcgag caaaccgcgg
    16501 tggtgctgct ttcgcacgtc aactaccgca ccggctacca gtgggacctg tccgccgcca
    16561 gccagcaggc ccacgccgcc ggcgcgctga tcgtctggga cctgtgccac tcgatcggcg
    16621 ccgtgccgat cgacctcgag gccgccgacg ccgactttgc cgtcggctgc acctacaagt
    16681 acctcaacgg cggccccggc tcgcccgcca tgctgtgggc caagccgcgc caccgggccg
    16741 atttctggca gccgctgtcg ggctggtggg gccatgccag gcccttcgac atggcggtcg
    16801 actacgcgcc ggatcggggt atccgcggct tcctgtgcgg tacccaaccc atcgtctcgc
    16861 tgggcctggt ggcctgcggc ctggagatct tcgccaagac cgacatgcac aagctgcggc
    16921 gcaagtcgct ggcgctgacc gacctgttca tcgccctggt cgagcaggag tgcgccggcc
    16981 agggcctgac cctggtcacc ccgcgcgagc acgcgcagcg cggcagccat gtcagtttcc
    17041 gccaccccga gggctacgcc gtggtgcagg cgctgatcgc gcgcggcgtg atcggcgact
    17101 accgcgagcc cgaggtggcg cgctttggtg tgaccccgct gtacctgcgc tttgtcgaca
    17161 tctgggatgc ggtgcagcac ctcaaggcag tgctgaccca gcgcgaatgg gaccagcctc
    17221 ggtaccgcca acgtggcgcc gtgacgtgat cgcagcatcc tgaaggaaac ctccatgacc
    17281 gccactttca accgctcaac ccaggccagc gtggtggccg gcaaagccac gccgcgcggg
    17341 cgctttccgc acatcaagcg ggcgggcgac tttctttttg tgtcgggcac cagctcgcgc
    17401 cggccggaca acagcttcgc tggcgtgcag gtcgacgcgc tgggcgtgac caacctggac
    17461 atccgggcgc agacgcgcgc cgtgatcgag aacattcgcg acatcctgct cagccagggt
    17521 gcgcagctgt ccgacctggt ggaagcccag gtgttcctgg tcaacatgaa cgatttcggc
    17581 ggcttcaacg aaacctgggc agagtttttc gactacgaag gcccgacgcg caccacggtg
    17641 gcggtgcatc agctgcccca cccgcatctg ctgatcgaaa tcaaggccac cgcctaccac
    17701 ccccggcccg gcacgggcca gccacgctga aggagacgac catgcagccg aaatacggcc
    17761 tccccaagaa cctgatggac tgggtcggcg cccacagcga ccgcctgcgg ccaccggtgt
    17821 gcaatgcggc cctgtttcct gacggcaact acatcatcaa catggtgggt ggtggcaaca
    17881 cgcgcaccga cttccatgac aacccgacgg aagaaatctt ctatcagatc cggggcaccg
    17941 cctacctcaa catctgggac cgtggcaagt tcgatcgcat cgacctgaag gagggcgacg
    18001 tctggctgat ggagccgcat ctgcagcact cgccgcagcg ccccgacccc catggcctgt
    18061 gctttctggt cgagctcaag cgaccccagg gcgccatcga cacgctcaac tggtactgcc
    18121 cccgatgtgc ggctctggtc tggggagcct cgatggagct gaaagatctg gtggccgacc
    18181 tgccggccac ctaccagaag ttctacgccc tgagcgacgc cgaacgcacc tgtccgcact
    18241 gcggcaccgt gcaccctggc aaggactacg ccgcgtggca cgccatccgt gaggcccacg
    18301 catgagcggc gccttgtcga tgatcgatat ccatgcgcat ttcctgccgc agatcggcca
    18361 gcaggaagcc gccgcggtgg agcgcgagcg ggcgccctgg ctgcgcatcg acgccggtgg
    18421 cgaaaccggc cagatcatgg tgggcgagcg cgagttccga cccgtctatc gggcgttgtg
    18481 ggacgcggat ttccgcgtcg ccgaactgga cgcccagggc attgcgctgc agctcgtgtg
    18541 cgccacgccg atcatgttcg gctacgcctg ggaggccggg cgtgccgctg actgggcggc
    18601 gcagatgaac gacaaggcgc tgtcgttctg cgcccgtcac ccgacacggc tgaaggcgtt
    18661 ggcccaggtg ccgctgcagg acaccgcacg ggcgtgcgcg gaagccagcc gggccatggc
    18721 cgccggttgc gtgggtgtgc agatcggcaa ccacgtgggc gacaaggacc tggaccaccc
    18781 cgacctggtc gacttcctga ttcactgcgc ggaccaccgc attccggtgc ttgtccaccc
    18841 ctgggacatg atgggcgggc ccgggcgcat gaagcaatgg atgctgccct ggctggtgtc
    18901 gatggccgcc gagacacagc tgggcatgat gtcgctgatc ctctccggcg cgctggagcg
    18961 cattccgaca tccctgcgta tctgctttgc ccacggtggc ggcagttttg cctatctgct
    19021 gggccgcgcc gacaacgcct ggcggcagcg cgacatcgtg cgcaaggatt gcccgcgccc
    19081 gccgtccgag tacgcgcgcc gcctgcatac cgacgccatc gtgttcgacc cgcgggcctt
    19141 gcacctgttg atcgacgtga tgggggtgga caacgtcatg ctcggctcgg actacccgtt
    19201 cccgctgggc gagcagcagg tcgggtcgct ggtgcgtggg gtcgacttcc tgtcgggcag
    19261 ccagcgcgcg cagatcctga gcgacaacgc gcgccgcttc ttcgccctgc ccgaggggtg
    19321 agcggcacac gctatatttc aaccgtcaaa caacaactgg agacaacaga tgaaatccac
    19381 tgtgcgcaac gtgattgcag ccgcggcact gtccatgctg gcgagccacg ccctggccga
    19441 cgtgaagatc gggttcatgg ccaccatgtc gggcagcgcc gccgcgctgg gtcaggacat
    19501 gtacgacggc ttcatgctgg cgctggagca gaacggcggc aagctcggcg gccagaagat
    19561 cgatctgatc aaggaagacg atcagctcaa acccgatgtg ggcgtgcaga tcgtgcagaa
    19621 gttcatcgag aaggacaagg tggatatcgt tgccggcgtg acgttttcca acgtcatgat
    19681 ggccattgcc aaaccgctcg cggacgccga catgacctac gttggctcca acgccggtcc
    19741 cgcgccgctg gcgggcaagc agtgcagcaa gggtttcttc ttcgcctcct ggcagaacga
    19801 tcagcaggcg gaagtcatgg gcgccttcgc caacgacaag ggctacaaga aggcctacct
    19861 gctggcgccc aactaccagt cggggcgtga ccagatcgcc ggcttcaagc gccagtacaa
    19921 gggcagcgtg gtgggcgagg tgtacacgca gctgaatcaa cccgattact ccgccgaact
    19981 ggcgcagctg caggccgcca gcccggacgt ggtgtacgtg ttctatcccg gcggcatggg
    20041 cgtgaacttc atcaagcagt accggcaggc cggcctgctg ggcaaggtcc ccctggtgtc
    20101 cgcctcgacg gtggacggta ccacgttgcc ggccctcaag gagatcgcgc tgggcgccgt
    20161 caccggcacg ttctgggggc ctgacttcaa caacgccacc agcaaggcct tcgtcgccgc
    20221 gttcgagaag aaatacaacc gcatcccctc gcagtacgcc gcacaggcgt acgactcggc
    20281 attgctgatc gacagcgcgc tcaagaagac cggcggcaag gtggcggaca agcaggcgct
    20341 gcgcacggcg ctgcgggcgg ccgactacgc ctcgctgcgc gggcccttca agttcgatgt
    20401 caacggattt ccgatccagg atttccatgt cttcgaggtc gccaaggaca gtgctggccg
    20461 ggtctcgctc aagaccatcg ccacgcccct cagggcacac cgtgacgcct atgtgggcga
    20521 atgcaagctc tgatgccttc accgctcgaa agggttgacg ccgatgcgga ccgtccgcat
    20581 gcggtgcatg cgttgggtcg ggctgaccgt tcgggccggc gcacgcgcct gggcatggcg
    20641 ggcgagcggt cgtcgccggc aggggcggtg ccgcgggcag gtgacggccc gatgcccgca
    20701 ggccgatgca cggcgctgca acccggttgc gcggcaaggt gagcccgtgc ctgtgatgtc
    20761 gtcccgcgtg gatcgccggg tgccgcatga cacctgagct gctgctgata cagacgctca
    20821 atggcctgca gttcggggtg ctgctgtttc tgatggcggc cggcctgacg ctggtgttcg
    20881 gcatcatgag cttcgtcaac ctggcgcacg gcgcgctcta catgttcggc gcctacttcg
    20941 ccgccgccgc ctggaacgcc agcggatcct tcctgctggc ggtgctgggg ggctttggtg
    21001 gagcgttcgt gcttggcgcg ctggtcgatc gcgtcggtct gtttgccctc tacccgcgcg
    21061 accacctgga ccatgtgctg gccaccttcg gtctggtgct gtttgccaac gaactggcgc
    21121 gcatgatctg gggcgcctcg ccggtgttca tggaactgcc cgaatcgctt gcggcttccg
    21181 tgcccttgct gggcatcaac tacccggtgt accggatgct gatcatcgtc gtcgggctgg
    21241 ccgtggcggc gtgtctgtat ctgctgatcg agcgcacgcg cgtgggcatg ctgatccgtg
    21301 ccggtgccag caaccgcgcc atggtgtcgg cactgggggt gaacatcggc tggctcaaca
    21361 cattcatctt cgcgctgggc gccggtctgg ccggtctggc gggcgccatg gtgggcccga
    21421 tcatgtcggt gcagccgggc atgggtgatc cgatcctgat caccacgctg gtggtcatcg
    21481 tgatcggggg catcgggtcg atccgtggtg ccttcctggg cgcgctggtg gtcggcgtgg
    21541 tcgataccgt ggggcgcacg ctggtgccgg cgctgctgcg cggcgcgctg gcgcccgaaa
    21601 tggccaacac gctggggccg gcgctgtcgt cgctggccgt ctatgtgctg atggcggtgg
    21661 tgctggcgct caagccggat ggcctgtttc cggtcaggaa gcgctgagcc cagcaacggt
    21721 cctctatcac ttcccctcat cctgagagcc gcatgaacac tgggattcac tgcttcgctg
    21781 gcctgcacct gccgggtgag tgcgcggccc gaccgtcagc atggcgtctg ccacgccttg
    21841 cgggcggcgc cctgcggcgt cgccgcgtgt tgacggtcgc gccgatcatg gcgcgggcgg
    21901 acgcgcccag cggctgtttc cgggactgaa ggggcggaac atggtttcgt cttcggtggg
    21961 tgtgcacgct gcggatcgcc tggcaccgac gttgctgggg ctttccaagg cgcgctggcg
    22021 aaccgtcctc acggtgttgc tggcggcctt cttcgtgctg gtgccgctgt acgcctggct
    22081 gaccggcgac agctacgtcc tgatcctgat gggccgcatt ctggtgtttg cgctggctgc
    22141 ggtgggactg aatctggcgt tggggtacgg aggcatggtg agtttcgggc atgccatgta
    22201 cctcggcatc ggcgcatatg tggtggccat cgcttcgcac catggcctgg acaatgggct
    22261 ggtgcatctg ctgctggtgg ctgccctcac ggtggcggtt gccatcccgg tggggctcat
    22321 tgcgctgcgc acccagggca ttgcgttcat catgatcacg ctggcgtttg cgcagatgtt
    22381 gttcttcgtc gccgtaggtc tgaagcagta tggcggcgac gaggggttga gcatcgtcca
    22441 tctgtctcgc ttcggcgccc tgacggggaa caagaccgcc ttgtacctgt cgatgctgct
    22501 tgcgctgggc ctggcgctgt ggctgttgcg tcgcgtcgtg gcgtcgcgtt ttggcctggt
    22561 gctgcgcgcc acgatgatca atgaaaggcg ggtgcgcgcg gtcggcacgc cgccactggg
    22621 ctatcgcctg gcgggctatg tggcgtcggc cctgctgtgc gccctggcgg gttatttcct
    22681 ggccaacctg acctcgtttg cgtcgccggc ctacatggcc tggacggttt cgggcgagct
    22741 catcgtgatg gtgttgctgg gcggcatggg cacgctggtg gggccggtcg taggggcgct
    22801 ggtgtttctg ctgctggagg aggggctcaa ggcgctcacc gaccactggc tggtcatcat
    22861 gggtccggtc atcgtcgtca tcgtgctgtt tctgaagaac gggctgtggg gcatcgtctc
    22921 cgacgtgccg gcagccaggg gagggcattg aagtgacgca gtcgcagccc ttgctgagcc
    22981 tgcggggtct ggtcaagcgc tacggtggct tgctggtgac cgatcatgtc gacttggacg
    23041 tgctgcccgg cgaggtgcat gccatcatcg gccccaatgg cgctggcaag accaccctga
    23101 tcggccagat cgtgggagag gtggccagcg acgagggcag catccggctg caggggcagt
    23161 ccatcgacgg actgagcgtg cccgagcgcg cccgcgcggg ccttgggcgc tcctatcaga
    23221 tcacctcggt catcggcgct ttcaccgtgc tggaaaacgt gattcttgcg gtgcagggct
    23281 gcctgggtca tgccttccat ttctggcgac cggccagcga aacggctgaa ctggtggagc
    23341 gtgccgatga ggtgatcgag cgggtcgggc tgagtgcgca gcgtgatgtc catgcggcgg
    23401 cgctcgccta cggtcagcaa cgccagctcg aaatggccat ggctctggcc atgcagccca
    23461 gcgtgttgct gctggacgag cccatggcgg gcatgggggc ggtcgaatcg tcgcaactgg
    23521 tggctctgtt gcagcgcctg aaggggcagt acggcattct gctggtcgag cacgacatgg
    23581 gggcggtgtt tgccctggcc gatcgcatca gcgtgctgtt tcacggcaag gtcatctgct
    23641 gcggtgaccc tgcgaccgtc aaggcgcacc cactggtgcg ggaagcctat ctgggcgacg
    23701 aggagattcc ggcgtgaacc tgcttgaggt ggaaggcctt gccgccggct acggtcagag
    23761 ccaggtgctg ttcgacatcg gcctggcggt ggcacccggt caatgcgtca ccttgctggg
    23821 gcgcaacggc atgggcaaga ccaccaccat caagaccatc atggggcttc tgcgcccatg
    23881 gggtggcgtg gtgcgctgga aggggcgcgc cgtccagggg cgcccccccc atgcgatggc
    23941 gcggctcggc ctgggcctgg tgccggaagg ccggcaggtg tttcccaacc tcaccgtgcg
    24001 cgagaacctg gtggccaccg ggcgcggcgg cggttcgtcc catgcctgga cgctggcgcg
    24061 ggtgcacgcg ctgtttcccc gcctggccga acgggcgtcc aacttcggca accagctgtc
    24121 cggcggcgag cagcagatgc tggccattgg acgtgctctg atgaccaacc ctgagttgct
    24181 catcctggac gaggccacgg aagggttgtc gcccctggtg cggcgtgaga tctggcttgc
    24241 gctgcgacag atcaaggccg aaggcatggc gattctggtg gtcgacaaga acctggcgcc
    24301 gttgctggaa ctggctgagc gccaccacat cgtcgagaag ggccaggtgg cctggagcgg
    24361 cagctccgag gaactgcgcg accagcggtc cctgatgcag cgctacctgt cggcttgaac
    24421 agggcagcct ttggtgcagc aggtttgcct tcggccgaca aaggcattaa actgctaggt
    24481 tacccgggcc cttggcggcc cgggccatct tccgcccagt gctgcaaccc gcttcggtgt
    24541 gagcgcagcc gtgttgggcg gactttcgtt tccgcgcagg cgcgccgttt tcggatgggg
    24601 cctggtcagc ggtttccgtg gccgctgccg ctgggcggcg tgccctgttt gccaccttgc
    24661 tcgtggagat gtgtcatgcg cgttctgcct cacaccgatt cccccacccc cgtggttgcc
    24721 tcaggcgcca cgctgcgcag cgcgcatgcg gttgcgcgca tggcgcccgg tctgcatctg
    24781 attggcgatc tgtacggctg tcgtggcgac acccgactga tgaccgacgc cgccacgctg
    24841 gaggcctttt gcaagcaggc cgtggccgac gccggcctga ccacggtggg cagcctgttt
    24901 cacagctttg gtgaaggcga gggcgtgacc ggggccgtgg tgctggccga gtcgcacctg
    24961 gccctgcaca cctggcccga agacaactac gtcacgctcg acgtgtacgt gtgcagctac
    25021 accaacgaca actctgccaa ggccgagcgc ctgttcgacg ccttgatgca ggcctttcag
    25081 ccggccgatc cgcatctgca ccgcgtcgtg cgcgcatgag cgcgccgatg agcgcgtcga
    25141 tcggcaccgg cggccaattc gccgtcgagc agttgtcgcc cgacttcggc ttctatctgc
    25201 gtaccaccgg gttgctggcc gagcgccgct cgtccatgca gcacatcgaa atcgtggaca
    25261 cgccgctgtt cggccgcgcc atgcgcatcg acggttgctt catgacctcg gagcgcgacg
    25321 agttcttcta tcacgagccg atggtgcacc tgcccgccat cgcccatggc gatccgcgcc
    25381 aggcgctggt cgtgggcggg ggcgacggtg gcgcggcgca ccatctgctg cgctacccga
    25441 acatggcgcg ggtggtgctg gccgagctgg accgtgacgt gatcgacatg gcgcgcgcat
    25501 ggctgccggc cgtgcatcgc ggcgcttggg atgaccctcg cctggagctg cgcctgggtg
    25561 acggccgcgc cttcatcgag tcgtgcgaga gccggttcga ccagatcgtg cttgatctga
    25621 ccgacccttt cggccccgcc gtggcgctgt acacctgcga gttctaccgt gcctgccggc
    25681 gtgcgctgcg gcccggtggg gtgctgtcgc tgcacgtgca gtcgccgatc catcgcggcg
    25741 cgaccatggc gcgcctgctg gcgtcgctgc gcagcgtgtt cccggtggtg cgaccgtttc
    25801 tgcagtacgt gccactgtac gggacgttgt gggcgatggc gatggcgtcc gaccgcgccg
    25861 acccgctggc gctgacggcg gccgaggtcg acgcccgtct ggcgcgccac ggcctgaacg
    25921 atctgcagct ctacagcggc gacacgcacc tggcgctgct cagcctgccg ccgttcgtgc
    25981 ggcgcctgct ggccgagccg gcgcgcccgg tggtggacgg cgacagcctg gacgatccga
    26041 gcctggaccc cggcgcagag cgcacgctgc ggctggtgcg cggctgagcc gcgcccgcag
    26101 cggtgtcggt cgcgggcgaa attctacaat ttgcgtaatt gcgcaaacag tcgtagcata
    26161 cgaccctcgt caacttgtgg agtccatgcg atgaagaagc tgctgatcgc ggtggcgctg
    26221 gggctggccg gctggtccgc cggggccgtg gagacgggca tttcagcccg cgaggcgtat
    26281 gaaaaggtgc agagcaatgc cccgggtgtg ctgttcgtcg acgtgcgcga cccggtggaa
    26341 atcatgttca ttggcttcac cgatgtggtg cacaccaaca tcccgttcat gatggtggac
    26401 cgctcgaaat gggacgagca gcgcgggata ttccggctct acccgaaccc cgatttcgtg
    26461 gcgcagatca aggccgagct ggcgcgccgc ggcatgggca tggatgccga ggtcatcacc
    26521 ctgtgccgct cgggcagtga gcgcggtgag cccagcgcgc gtctgctgcg cgaaaacggc
    26581 ctgccgaacg cgcgctacgt gatcaacggc tttcagggcg cggcgctcaa ggacgggccg
    26641 caggccggcc tgcgcatcca gaacggctgg caaaacagtg gcctgccctg gagcgccaag
    26701 atgagtccgg gcaaggtgta ccgcgtcgac cgccagcccg gcaactgagt cgccggtacc
    26761 agtagggtgc gggcagggtg ctgaccgcgc tataatttca aggctttgcg tgttgcagag
    26821 cgcacgtggc gtgcagttcc ggcgcgccac gcaagtcaaa ccctttggaa cgaggacatt
    26881 gagatgatct ccaaggagaa caaggccgcc gtggtcaaag acaacgcgcg cgccgccaat
    26941 gacaccggca gccccgaagt tcaggtggcg ctgctgacgg cgcgcatcaa cgagctgacg
    27001 ccgcacttca aggaacacgc caaggaccac cacggccgcc gcggcctgct gcgcatggtc
    27061 agccgccgtc gcaagctgct ggactacctg aaggccaagg acgccgaccg ctacctggcc
    27121 ctgatcgcca agctgggtct gcgcaagtaa gcccgtgatg agcgcaaacg cctgacgcct
    27181 cccagggctc aggcgttttt gtcttttttc aacccggagc gcggggtttt cagaagctga
    27241 agctgtgtca ttccacgacc tttcgtcctt caccggcagg ttctggaatg gcatcgtgtt
    27301 ctgaaacaaa tccggctcca acgtttgtgc agcaagcgca aacagctatc aatataggag
    27361 caatcatgag catgttcaac aagatcacca agtcgtttca gtggggcgac aagacggtgg
    27421 tgatggaaac cggcgagatc gcccgccagg ccaccggtgc cgtgctggtc gacatcgacg
    27481 gcaccgtgat tctggccacc gtggctgcca gcaagagcgc caagccgggg caggacttct
    27541 tcccgctgac ggtcgactac atcgaaaaga cctacgccgc cggcaagatc ccgggcagct
    27601 tcttcaagcg cgaggccaag ccgagcgagc tggagacgct gacgtcccgc ctgatcgacc
    27661 gcccgatccg cccgctgttc cccgaaggtt tcttcaacga agtgcatgtg gtgatccaca
    27721 ccgtctcgct caaccccgag gtggatgccg acatcgcggc catgatcggc gtcagcgctg
    27781 cgctgtcgat cagcggcatc ccgttcaacg gcccgatcgg tgccgcgcgg gtcggttaca
    27841 tcaacggcga atacgtgctc aacccgggtc cgacggcgcg caagagcagc cagctcgacc
    27901 tggtggtggc tggcaccgag gccgccgtgc tgatggtgga aagcgaagcc gatcaactgt
    27961 ccgaggacgt gatgctgggc gccgtggtgt tcggtcacca gcagggccag atcgccatca
    28021 acgccattca cgagctggtg cgcgaagccg gcaagccgct gtgggccgac aacggcacct
    28081 gggtgccgga agccaaggac caagccttca tcgcccaggt caacgccgcc gccgagggcc
    28141 cgctgcgcgc cgcctaccag attcgcagca agcaggcgcg cacccaggct tgccgcgagg
    28201 cctatgccgg cgtcaaggcc gcgctgaccg agcagggcgt ggcgttcgac ccggtcaagc
    28261 tcgacaacct gctgttcgag atcgaggcgc gcatcgtgcg cggccagatc ctggccggcg
    28321 agccgcgcat cgacggccgc gacacgcgca ccgtgcgccc catcgaaatc cgcaacagcg
    28381 tgctgccgcg cacccacggc tcggcgctgt tcacgcgcgg cgagacgcag gcgctggtcg
    28441 tcaccacgct gggcaccgag cgcgacgcgc agcgcatcga cgcgctggcc ggcgagttcg
    28501 aggaccgctt cctgtttcac tacaacatgc cgccgtttgc cacgggcgag gtgggtcgca
    28561 tgggctcgac caagcgccgc gaggtcggcc acggtcgcct ggccaagcgc gcactgatcc
    28621 cgctgctgcc agccaaggat gagttcccct acaccatccg cgtggtgagc gagatcaccg
    28681 aatccaacgg ctccagctcg atggcctcgg tgtgcggtgg ctgcctgtcg ctgatggacg
    28741 ccggcgtacc catgaaggcg cacgtggccg gcatcgccat gggcctgatc aaggaagaca
    28801 acaagttcgc cgtgctcacc gacatcctgg gcgacgagga ccacctgggc gacatggact
    28861 tcaaggtggc cggcaccacc gccggcatca ccgcgctgca gatggacatc aagatccagg
    28921 gcatcaccaa ggagatcatg caggtggcgc tggcgcaggc caaggaagcg cgcatgcaca
    28981 tcctgggcaa gatgcaggag gccatgggcc aggcgcgcac cgagatctcg agctacgcgc
    29041 ccaagctcta cacgatgaag atcaaccccg agaagatccg cgacgtgatc ggcaagggcg
    29101 gcgccaccat ccgcgccctg accgaagaaa ccggcaccac catcgacatc gccgaggacg
    29161 gcaccatcac catcgccagc accgacgccg ccaaggccga ccacgcccgg cgccgcatcg
    29221 aggagatcac cgccgaggcc gaagtgggca aggtctacga aggcccggtc accaagctgc
    29281 tggagttcgg cgccctggtc aacatcctgc ccggcaagga cggcctgctg cacatcagcc
    29341 agatcgccca cgagcgcgtc gaaaaggtca gcgactacct gcaggaaggc caggtggtca
    29401 aggtcaaggt gctggagacc gacgacaagg gccgcatcaa gctgtcgatg aaggcgctgc
    29461 tggagcgccc ggcgcatctg gaaggcagcg acgagcgtcc gccgcgcggc gatcgtcccc
    29521 cgcgcggtga gcgtggcgag cgcagggagc gtgcgccgcg tgaggatcgc ggtgagcgcg
    29581 aagagcgtcc ggcacccgcc gccggcgcac ctgaggccgc ccccgagctg ccgtcgccgc
    29641 agatctgatt gctatataaa gcatagcttt tggcgcagta cggacgagcg ctggagccct
    29701 atctggccat gaaatgcatc gagatccgcg aacccggcga gccggccgtg ctggtgccgg
    29761 ccgagcgccc gctgccgcag gccggcccgg gtgagctgcg gatccgcgtc agcgcctcgg
    29821 gcgtcaatcg cccggacgtg ctgcagcgcg ccggccacta cccggcgccg cccggtgcgt
    29881 ccgacctgcc cgggctggaa gtggccgggg tgatcgagtc cggcgacgcc gctgcgctgt
    29941 ctgcggccgg cttgcagctc ggccagcgcg tgtgcgcgct ggtggccggt ggcggctatg
    30001 ccgaggtttg cgtggccccg gtggcgcagt gcctgccggt gcctgcgggc tgctccgacg
    30061 tcgaggcggc ggcgctgccg gaaacctttt tcactgtctg gagcaacgtg ttcgaccgcg
    30121 ggcgcctgca ggccggcgaa accctgctgg tgcagggcgg caccagtggc atcggcgtca
    30181 ccgccatcca gctggccaag gctgcgggcg ccaccgtgat cgtcaccgcc ggttcggacg
    30241 acaagtgtgc cgccgcactc cgcctgggcg ccgaccacgc catcaaccac cgcacgcagg
    30301 acttcgcgca gcaggcgctg cagctcaccg gcgggcgcgg cgtcgacgtc atcctcgaca
    30361 tggtggcggg cgactacgtg cagcgcgagg tcgaatgcct ggccgaagac ggtcgcctgg
    30421 tcatcatcgc cgtgcagggc ggcacgcgca gccagttcaa cgccggtctg gtgttgcgcc
    30481 ggcgcctgac gatcaccggc tccaccctgc ggccacgccc ggtcgcgttc aaggccgcca
    30541 ttgcgcaggc cctgcgccag cgcgtctggc cgtggctgga gcaggggcgt gtcaggccgg
    30601 tgattcacca ggtatggccg gcgctggagg cgggcgatgg cctgcccagc ggcgctgccc
    30661 gggcccatgc cctgatggaa agcggtcagc tgatcggcaa gcttgtgctg acctggagcc
    30721 aaccatgagc gaaagaaaga aactcgttgc cggcaactgg aagatgaacg gcagtctggc
    30781 ggccaaccag gcgctggtgc aggcgctgcg ccagggtgtc ggccagccgg cgtgcgacgt
    30841 cgccgtgtgc ccgccctcgg tctatctggg gcagctgcag caactgctgg cgggccagac
    30901 ggcgattgcg ctgggcgcgc aggacctgtc gcagcacgaa ggcggtgcct tcaccggtga
    30961 cgtctcggcc accatgctga aagacttcgg cgtgcgctac gccatcgtgg gccacagcga
    31021 acggcgccag caccagggcg agaccgacct gcaggtggcg ctcaaggtca ggcgtgcgct
    31081 ggcggccggc atcacgccca tcgtctgcgt cggcgagacg ctgcgcgagc gcgaagaggg
    31141 cctgaccgaa ttcatcgtca agcgccagct gtcggcggtg ctccatctca acggtcacgc
    31201 catcaatgaa accgtggtcg cctatgaacc cgtgtgggcc atcggtaccg gccagaccgc
    31261 cacgccggcg caggcgcaga gcgtgcatgc ggtgctgcgt gcgcaactgc gcgccgccgg
    31321 tgcccaggcc gaacacgtgc gcatcctcta cgggggcagc atgaacgcgg gcaacgccgc
    31381 cgagctgctg gcgcagcccg acatcgacgg cgggctgatc gggggcgccg ccctcaaggc
    31441 cccggatttc ctgaccatcg tggccgccgc cgcatgacgg gcgtgcgcca catgctattg
    31501 attttggagt aactggaatg aatgtcatct tgaacctgat cctggtggtg cagctgctgt
    31561 cggcactggc gatgatcggt ctgatcctgg tgcagcatgg caagggtgcc gacatgggca
    31621 cggccttcgg cagcggcgcc tcgggcagcc tgttcggggc caccggcggc gccaacttcc
    31681 tgtcgcgcac cacagcggtg ctggccacgg tgttcttcgt ctgcaccctg gcgctggcgt
    31741 atttcggctt cagcgtgcgc agcgcgccgg ccgacagtgg cagcgtgctg gaccgtgccg
    31801 cggtcacggc gccggccgcc ggtgcctcgg cggcggcgcc cgccaccgcg gccgacgcca
    31861 tcccgtccgg cggcacgccg gcaccggcca ccgagccgtc caggcccgcc acaccggcgg
    31921 caccggccgc cagcggagcg ggtcagattc cgggccagta aggggctcgt cagcccttga
    31981 aaaatcgacc gtttcctctc tgtaacggcc ggttttcagg gtaaactctt agctgctcct
    32041 gcgagtgcga aggcgttcac gctgacgtcg cgcaagagcc acccaatgcc gtcgtggtga
    32101 aattggtaga cacgctatct tgagggggta gtggcgaaag ctgtgcgagt tcgagtctcg
    32161 ccgacggcac cagaacgaca acgaaccgcg ggtgtcccag gtccgtggtt cacccagacg
    32221 aagaagcccg atgaatctcg ctcagtatct ccccgtcctg ctgttcatcc tggtgggtct
    32281 ggctgtcggg gctctcctgc tgggcatggg caacttcctc aagccgcaca aggccgatgc
    32341 ggccaagaac tcgccctacg aatgcggctt cgaagccttc gaggacgcgc gcatgaagtt
    32401 cgacgtgcgc tactacctgg tggcgatcct tttcatcctg ttcgacctcg agatcgcgtt
    32461 cctctttccc tgggcggtgt cgctgcgtga ggtgggcctg gtcgggtttg ttgccgtgct
    32521 catctttctg gccatcctgg tggtcggctt cgtctacgag tggaagaagg gcgcgctcga
    32581 ttgggagtga gccgcgcgcc cggcgcgcgc catgacagga catccgcaca tgaattcaga
    32641 gtttcagcaa aagggcttct tgctcaccgg cgtggacgcg gcggtgaact gggccaagac
    32701 gggctccatc tggccggtga ctttcggtct ggcctgttgc gcggtggaga tgatgcacgc
    32761 cgccgcatcg cgctatgaca ttgcccggtt tggcgccgaa gtgtttcgcg ccagtccgcg
    32821 ccagtccgac ctgatgatcg ttgccggcac tctgtgcaac aagatggcgc cggcactgcg
    32881 caaggtgtat gaccagatgg ccgagccgcg ctgggtgctc agcatgggct cctgcgccaa
    32941 cggtggcggc tattaccact acagctattc cgtggtgcgc ggctgcgacc gcgtggtgcc
    33001 ggtggacgtc tacgttccgg gctgcccccc gacggccgag gcgctgctgt acgggatcat
    33061 ccagctgcag cagaaggtgc gccgcaccca caccatcgcg cgggtatgag catgaccaag
    33121 cacgccattg cccctgaagt tctgaacgac accgtggcca gcgcgctggg cgagctggtg
    33181 cagcacatcg acgtggcccg aggtgaggtc acggtcagtg tgtcggcggg cgaataccac
    33241 caggtgatgc gcctgttgcg cgacgccgcc gggtgccgtt tcgaacagtt gatcgacctg
    33301 tgcggggtgg attactccac ctatggcgat ggccgttggg aaggcccgcg ctttgccgtc
    33361 gtcgtgcatc tgctgtcggt cagtctgaac cagcgcctgc gtgtgcgtgt gttctgcacc
    33421 gacgatgaat tcccggtcat ccgctcggtc accgacctct ggagcggggc cgactggttc
    33481 gagcgcgagg ccttcgacct ctacggcatc gtgttcgagg ggcacgccga cctgcgtcgc
    33541 atcctgaccg actatggctt catcggtcat ccgttccgca aggatttccc gctgaccggt
    33601 catgtcgaga tgcgctacga cgccgagcgc cagcgcgtga tctacgagcc ggtgacgatc
    33661 gagccgcgcg aaatcacgcc gcgcgtgatc cgcgaagaca actacggcgg cc
//
LOCUS       DAOOOG010000010        31575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_1208_length_31575_cov_25.258058, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000010 DAOOOG010000000
VERSION     DAOOOG010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 31575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31575
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1208_length_31575_cov_25.258058"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PK242_01810"
     CDS             complement(<1..748)
                     /locus_tag="PK242_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOZ92789.1"
                     /translation="MATALTDKLSGLVKKISGQARITESNVQDMLREVRMALLEADVA
                     LPVVRDFIARVKDKALGQEVLGSLKPGQALVGIVNRELAATMGEGVSDLNLAAQPPAV
                     ILMAGLQGAGKTTTTAKLARHLIQKRKKKVLTVSGDVYRPAAIEQLKTVTQQAGADWF
                     PSSPEQQPVEIARAALDHAKKHFYDVLLVDTAGRLAIDEALMQEIKALHAALNPVETL
                     FVVDAMQGQDAANTARAFKEALPLTGIVLTK"
     gene            852..1661
                     /gene="ccsA"
                     /locus_tag="PK242_01815"
     CDS             852..1661
                     /gene="ccsA"
                     /locus_tag="PK242_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOZ92790.1"
                     /translation="MILASASPVSLGLAAAAALAYAVPALAARALGAAGARRVLLLAW
                     LLHGAQLAWGVLGSEPRFGFAPALSITVWLVLTVYAIESQLYPQMTARWALAGFGTLA
                     ILLALLFPGAHPPATGSPLLPLHWALGIAAYGLLAAAVAHGWLMQRAEDQMRHATQTV
                     DGGVPLLTLERLMFRFLGAGFVLLTLTMLAGALFGEQLYGASYTGWRWDHKRVFTLLS
                     WLTFGTLLAGRWWFGWRGRRAVRVLYIGAALLLLGYVGSRFVLEVLLGRVA"
     gene            1658..1885
                     /locus_tag="PK242_01820"
     CDS             1658..1885
                     /locus_tag="PK242_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HOZ92791.1"
                     /translation="MKLLIVLLAVLAAVWLWRRGRRPSAPPPSDRRGRGQALPMVRCD
                     RCGVHLPGADAVRGHRGSYCCVAHRRDAEGA"
     gene            1885..3624
                     /locus_tag="PK242_01825"
     CDS             1885..3624
                     /locus_tag="PK242_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOZ92792.1"
                     /translation="MSSRRAHEQATWFGTQLPTAPSLPALTLRADADPSPGFARLWSA
                     FMSARVLIALALLGLQLVLHVLARPGSPWLLGTSVAYALFTIAVRVMGEPRGPGRAFD
                     SQWLPTVGVDLLFFSALQLQPTPSPINYTPLLALPVLMAAIMGTRALALGTAALVTLM
                     LLGHAAWAASGSSWANTAELLQAGLTGAGLLVLALLTNQMAMRLAREEALARRNRVEA
                     HVQAMVNAMVIEALPDGVLVIDTEHLVRAANPAARLMLGSDLDVTPPVFSLEDNPAWG
                     QLKRIVRQTFSDAPVDATEVTLHHEDRMSSHLQVRTQRTPSIGDSGVSLCVMFLQDLR
                     ELEARLRTEKLASMGRMSAAVAHEIRNPLAAISQANALLDEDLHEPLQKRLADMVRQN
                     AERLQHIVDDVLDVARVQGQGGEFSPVPALALDAEVEDFCNEWAAQHGQVQRLLLRLG
                     AGDAHVQFAPEHLRRVLVNLLDNAARYASRRAGAIQVETLAVRHGPVRLMVWSDGPAM
                     EAAVQRHLFEPFFSSESRSSGLGLYICRELCERHGASIAHERNARAQGGQSHEGNEFF
                     ISFRRAHSGHSTF"
     gene            3647..5185
                     /locus_tag="PK242_01830"
     CDS             3647..5185
                     /locus_tag="PK242_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOZ92793.1"
                     /translation="MNASVTMPGAASILVVDDEPDLRTLYELTLLREGHRVSSAASLA
                     EARALLATQAYDLVVTDMRLTDGLGLELLREMAAAQRRERSIVITAYGSAENAVEALK
                     CGAFDYLTKPIDLKQFRGVVGAALQARSATPADDAAPAREPAPPTPAATPAPAAPAAA
                     ASGPGAVALARLVGESAAMRAVKQRIAKVARSMAPVLVCGESGTGKELVARAIHACSH
                     RSEGPMVPVNCGAIPENLLEAEFFGARKGSYTGATQDRVGFFQAARGGTLFLDEIGEL
                     PLAMQAKLLRAIQERRVRPLGETQEEPVDVRIVSATHKDLAAEVQAGRFRQDLYYRLN
                     VIEIVVPPLRERREDLPALCQTLLGRIAADAGIAVPTLSARQLQDIRTAPLEGNVREL
                     ENLLHRAVALGEAGASGFGELESGTPSPQAATDSIAISGPTVAPPDAPRTPPHTDLPS
                     NLESWLDAREREVLVRALDETGFNRTAAAARLGLNLRQMRYRMARLGITAPSDDEPGP
                     DEGK"
     gene            5172..5780
                     /gene="ampD"
                     /locus_tag="PK242_01835"
     CDS             5172..5780
                     /gene="ampD"
                     /locus_tag="PK242_01835"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HOZ92794.1"
                     /translation="MKASEPSASAPRRAAWQGGWYRAARALPSPNHGPRPAGSEVDLI
                     VVHSISLPPGEYGGDQVQALFTNTLDWDAHPYFQPLRGLQVSAHFYVRRDGRLWQFVS
                     CDARAWHAGVSCFRGRENCNDYSVGVELEGLEGGPFEPAQYATLARLCRALARRYPIE
                     AIAGHQHIAPGRKHDPGPGFDWRHLQGLLRWSARRFADGALG"
     gene            6106..9018
                     /locus_tag="PK242_01840"
     CDS             6106..9018
                     /locus_tag="PK242_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HOZ92795.1"
                     /translation="MQTPHTLPASVAGPPGTLAHDRAGTPTATTQTLTHYQIIRRNGA
                     VVGFEPAKIAVAMMKAFLAVHGTPGAASASVRETVEQLTQTVVRALLRSRPAGGTFHI
                     EDVQDQVELGLMRGGHHEVARAYVLYRERRAQERARQAAGSQPAAPVLHVLQKGELVA
                     LDVPALEALLNQACANLDGAVQSAPILAETLRNLYDGVPVEEVYKAAILAARTLIEKE
                     PDYSFATARLLLHTIAAEVLGEAVDLPTLASRYAGNFPDFIRQGVAGELLDERLLQFD
                     LARLGAALKPERDLQFDYLGLQTLYDRYFLHLNKRRIELPQAFFMRVAMGLALNEIDR
                     EARAIEFYELLSSFDFMSSTPTLFNSGTRRPQLSSCYLTTVPDELEGIYESIKENALL
                     SKHAGGLGNDWTRVRALGSHIKGTNGESQGVVPFLKVANDTAVAVNQGGKRKGAVCAY
                     LETWHLDIEEFLELRKNTGDDRRRTHDMNTANWVPDLFMRRVVEGGTWTLFSPVDVPD
                     LHELCGAAFEQAYVAYEARAARGEISPSRTLPAVDLWRKMLSMLFETGHPWITFKDAC
                     NLRSPQQHVGVVHSSNLCTEITLNTSDSETAVCNLGSVNLAQHLRDGRIDHDKLRKTV
                     STAMRMLDNVIDINYYAVKKARDANLRHRPVGLGIMGFQDALYALRLPYASDAAVQFA
                     DESMEAVCYHAYWASTELAAERGRYSSYRGSLWDQGVLPLDSLGLLERARGGHVEVDR
                     SSTLDWDALRAKMARDGMRNANCVAIAPTATISNIVGVDASIEPSFGNLSVKSNLSGE
                     FTVINQHLVRELKRLDLWDEVMVMDLKHFDGSLRAIDRVPADIRQLYATAFEIEPHWL
                     VEAAARRQKWIDQAQSLNLYMAGASGRKLDETYKLAWRRGLKTTYYLRTTSATQAEKS
                     TITSGRLNAVPHGDDEALAMRARAAPTTDVAATVAAQPATDIRFCAIDDPGCEACQ"
     gene            9152..10294
                     /locus_tag="PK242_01845"
     CDS             9152..10294
                     /locus_tag="PK242_01845"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HOZ92796.1"
                     /translation="MLTWDHETTLTTPQPRLQPVTESAPSTTRATDAHSGVATGSPRR
                     VNAADKRIINGQTDVNQLVPFKYKWAWEKYLATCANHWMPQEINMNRDIALWKDPNGL
                     TEDERRIVKRNLGFFVTADSLAANNIVLGTYRHVTAPECRQFLLRQAFEEAIHTHAYQ
                     YIVESLGLDEGEIFNAYHEIPSIRDKDEFLIPFIDAIMDPDFRTGTPEADQRLLKSLI
                     VFACLMEGLFFYVGFTQILALGRQNKMVGAAEQYQYILRDESMHCNFGIDLINQIKLE
                     NPHLWTPAFKAEVRALFEHAVELEYRYAEDTMPRGVLGMNASMFKGYLRHIANRRATQ
                     IGLDALYPNAENPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD"
     gene            10477..10932
                     /locus_tag="PK242_01850"
     CDS             10477..10932
                     /locus_tag="PK242_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HOZ92797.1"
                     /translation="MATAKKAPAKKAPAKKAAPAKKAPAKKAAPAKKAAPAKKAAPAK
                     KAAPAKKAPAKKAAPAKKAAPAKKAAPAKKVAAKKAAPAKKAAPAKKAAPAKKVAAKK
                     AAPAKKAAPAKKPAAKKAAPAKKAAAKKPAAQTTLSPQAAWPFPTASKP"
     gene            complement(11068..11796)
                     /gene="mtgA"
                     /locus_tag="PK242_01855"
     CDS             complement(11068..11796)
                     /gene="mtgA"
                     /locus_tag="PK242_01855"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOZ92798.1"
                     /translation="MGAVLRRILLLAVLGLLLLQLFFVGRVALMVVLDPRSTSFERSE
                     AWRLASTGTLRWRQQWRDYAQISDHLKRAVIASEDDGFANHEGVDWDAIEKAWQRNAA
                     AEEAAAERAAQNRPARPIRIRGGSTITQQLAKNLLLSGERSLWRKGQELVLTFALEQL
                     LDKRRILELYLNHVEWGEGVFGAEAAAQHYFRKSAAQLTAAESARLAVMLPRPRYFEK
                     LPRSAYLASRTRTILARMRSAELP"
     gene            complement(11861..12757)
                     /locus_tag="PK242_01860"
     CDS             complement(11861..12757)
                     /locus_tag="PK242_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOZ92799.1"
                     /translation="MLRSYKTLSTLQWALGLSVLLHAVLLTVRFVDPEGFKRLFQETT
                     LDVVLVNAASDEKPVKAQAIAQSSLAGGGEAADRRMASTPLPPSPHEAPGDAPVTENQ
                     RRIDAMLQQQEQLLAQVRQQLAAMPKPDPRQLSEDPEARAQEERRQQLARLLAAIEKR
                     VEEENSRPRKRYLSPATLGTTYAVYYDSMRRKIEAEGTANFPQMAGRKLYGELIMALL
                     INHDGRILDARVVRGSGQRALDKLAELIAARAAPFGNFTAAMRKDTDQFDVTARFRFT
                     REQTLETMLQADPLATSVRELK"
     gene            complement(12757..14826)
                     /locus_tag="PK242_01865"
     CDS             complement(12757..14826)
                     /locus_tag="PK242_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOZ92800.1"
                     /translation="MHVLFDEAGKFLAGRILSEAEASAQVELDSGKRVKVKAANQLLR
                     FDKPAPAELLAQAQAQAADVDPALAWEFAPEDEFGFAELAADYFGGGPGPVQQAAALI
                     ALHEAPHYFRRAGKGRFRKAPADVVAQALAAIEKKKQLAAQIAAWADALAQGQCPAPV
                     REQLYRILFRPDRNAPEYKAVVEASRATQLAPLALLQRAGAIESAYQFHWQRFLFENF
                     PQGTGFARLAAPVIREELPLAPVAAFSIDDSATTEIDDALSLQGLGTGTVVLGIHIAA
                     PGLAIQPGDALDDVARARLSTVYMPGYKITMLPDEVVDAYTLAEGAPVPAVSLYVTLD
                     EATLEIQDRQTRLERVPIAANLRHDQLDALITEDWLQGASVDGQIDPQRLPVEREQLS
                     FLYQLSRHLKARREQVRGKPETFTRPDYSFRLVGNDGAVPTGAEQVQITTRSRGAPLD
                     LIVAELMILANSSWGEWLKALGVPAIYRSQASLLPGIKVRMGARALPHAGLGVPSYAW
                     STSPLRRYVDLVNQWQIIAAARHGTAAALVAPFKPKDAALLAIISAFDAAYSAYGAHQ
                     ASMERFWTLRYLHQQGITELNASLLREQTGGAWLVRADELPLVFTVMGAQGLPRGTRV
                     RVRLGQLDDIALDVHGTVLEVLQDQAAVPTAAEAEDDDEGAAAGPIAIAVELDETEGA
                     RTDNPPS"
     gene            complement(14864..15391)
                     /locus_tag="PK242_01870"
     CDS             complement(14864..15391)
                     /locus_tag="PK242_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOZ92801.1"
                     /translation="MTPTPTPVRYDRISRWLHWGMAGLFAWIFVSAGAHALAERSALD
                     ALLWPTHKPVGTLLLALLLARVAWALARRRRPPAVSALARLGHLGLYALMLAVPAIGL
                     LRQWGSGRAFAPFGLPLLPGFDVSQKVEWTLQLGRLLHGNLGWALLALVLGHVAAALW
                     HERAGHTPVLARMAG"
     gene            complement(15529..16113)
                     /locus_tag="PK242_01875"
     CDS             complement(15529..16113)
                     /locus_tag="PK242_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HOZ92802.1"
                     /translation="MPTTHLLYLHGFRSSPQSAKARQMAARVRADHPGVTWWCPQLPP
                     SPREAAALIAQGSADWPAAHMAVVGSSLGGYYATWLAQRRGCRAVVLNPAVDPARDLA
                     AHIGEQTAWHDPAERFLFRPEYVDELRALAPGPLQAPERFLAVIAQGDELLDWREMAA
                     RYAGAALRLIEGSDHALSDFEHHIDAVLSFLDLL"
     gene            16207..17364
                     /gene="rodA"
                     /locus_tag="PK242_01880"
     CDS             16207..17364
                     /gene="rodA"
                     /locus_tag="PK242_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOZ92803.1"
                     /translation="MAVVFDKPSLWQRLWPLLRGFDLLLLVAVLVLAGMGLMTMYSSG
                     FDHGTRFADHARNMLIAAGIMFVVAQVPPQRLMSLAVPVYTLGVGLLVAVALFGITKK
                     GAQRWINVGVVIQPSELLKIATPLMLAWWFHRREGQTRPVDFLVAFALLAAPVGLIMK
                     QPDLGTALLVGAAGLAVIFFAGLPWKLVLPPVLLGALGVGLLLAYEGSLCADGVDWRV
                     LHEYQRQRVCTLLDPTRDPLGKGFHTLQGMIAIGSGGLWGKGFMQGTQTHLEFIPERT
                     TDFIFAAFSEEFGLAGNLALIAGFVFLILRGLMIAAEGPTLFARLLAAALTTIFFAYA
                     FVNMGMVSGMLPVVGVPLPFISYGGTAMVTLGLALGMLMSIAKARRTLMQS"
     gene            17498..17839
                     /locus_tag="PK242_01885"
     CDS             17498..17839
                     /locus_tag="PK242_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOZ92804.1"
                     /translation="MAGKFELKKSKNGKFFFNLLASNGQVILSSEMYEAKASAVNGIE
                     SVKKNGADAAHYEKLTGKDGSPYFTLKAGNGQVIGQSQMYASETTRDNGIQSCVSHAP
                     GATVDDQTAQA"
     gene            18023..19267
                     /locus_tag="PK242_01890"
     CDS             18023..19267
                     /locus_tag="PK242_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HOZ92805.1"
                     /translation="MKLLEPILAQAAAISQLRRDIHAHPELCFEEVRTADLVAAKLAE
                     WGIATHRGMGTTGVVGIVHGRDGGKSGRAIGLRADMDALPMTEFNTFAHASRHHGKMH
                     ACGHDGHVAMLLAAGQQLAATRDFDGTVYLIFQPAEEGGGGAREMVKDGLFDRFPMEA
                     VFGMHNWPGMEAGRFAVSAGPVMGSSNEFRIVITGKGGHAAMPHTGIDPLPIACQVVL
                     GFQTIISRHKKPVDPGVISVTMVHAGEATNVVPDACEIQGTVRTFSFETLDLIEQRMQ
                     AIAEGVSAAFGAQCTFEFTRNYPPTINTAYEAEFCRRVMAGIVGEANAVPQEPTLGAE
                     DFAYMLLEKPGAYCFIGNGMGAHRGRYEGGGHDAGPCTLHNPHYDFNDDLIPLGGTYW
                     VQLAQQWLAHQPPADRPLPRKG"
     gene            19440..20546
                     /locus_tag="PK242_01895"
     CDS             19440..20546
                     /locus_tag="PK242_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOZ92806.1"
                     /translation="MIPIPQAFAASYAQARVKFLEAAASASTQIESHRHPLPGRDGEL
                     LALDLALDGDAQSERLLIVSSACHGVEGFCGSGVQVFALHDADWRQQARDAGVAVLYL
                     HALNPWGFSHLRRVTHEGVDLNRNFPDFSQPLPVNTAYRQLHPWLLPPQWPPDAAHRA
                     AVQDFIAREGVAAWQTAISGGQYEFPTGLFYGGHAPTWSHQTLRGVLARRAGRARRVA
                     WIDLHTGLGPPGVGERIWAGGQNAAGVARARRWWGEQVTSIHDGSSTSPPLTGLMWSA
                     IDAAAPQAEYTGIALEFGTEPILEILDALRGDHWLAQHPEAPPALAADIKARLRRAFY
                     TDTDAWKGQIVSQARQALMQAVQGLSEGGRGTAA"
     gene            complement(20572..21573)
                     /gene="rssA"
                     /locus_tag="PK242_01900"
     CDS             complement(20572..21573)
                     /gene="rssA"
                     /locus_tag="PK242_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HOZ92807.1"
                     /translation="MTHPPPAHAAFARRAPVIGLALGSGSARGWAHFGVLRALTDCGI
                     RPGVVCGASIGALVGAAHVLGLTERFERWVLGLRRRDVVSFMDISTGGGLLKGEKLMR
                     FFRSQFTDRDFDALETPFAAVATALHTGEEVWLRQGSVVDAVRASIAMPGLFTPVWHQ
                     GRLLVDGGLVNPVPVSLARALGADIVIAVDLNADLIGRHLRHGPAQAAVPASEPSIGW
                     LQNLQRSLGWHRDEAAAQDATPPIPPVLEVLTSGIHIMQVRITRSRMSGDPPEFTIAP
                     RLSHLSLLDFHRAHEAIEAGEQAAQESLGLLRELGLCPPTRETTPAAPPDAASGATG"
     gene            complement(21634..22062)
                     /locus_tag="PK242_01905"
     CDS             complement(21634..22062)
                     /locus_tag="PK242_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOZ92808.1"
                     /translation="MTEPTACPEPPWRHILRDVPDALIYADAEGVIRAWNAGAEALFG
                     FSAAEALGASLDIIIPERLREPHWVAYRRAMARGATRGGAEVRTTRGTHRDGRKLYVD
                     MSFGVVRDDHGQVLGSVAMARDATARHLAEMARRTATGGT"
     gene            22215..23042
                     /locus_tag="PK242_01910"
     CDS             22215..23042
                     /locus_tag="PK242_01910"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOZ92809.1"
                     /translation="MSTPTPTRMPTLYIPHGAGPCFFMDWNPPDTWARTAAFLRGIPD
                     SLPAAPTAILLVTAHWLAPQPSLGSAAQPGLLFDYHGFPPHTYELSYPAPGAPALARR
                     AAALLTDAGFAPRLDDARGYDHGSFIPLKLAFPAADVPVLQMSLLRSLDAGEHLRLGQ
                     ALAPLRDEGVLIVGSGMSYHHMRGYGDPRSTPVAAEFDRWLAETVALPAAERHARLTD
                     WARASGPAGRLSHPAQAEEHLLPLHVAAGAAGDSRGRRVFTDRVMAVDISAFRFDDS"
     gene            complement(23298..24173)
                     /locus_tag="PK242_01915"
     CDS             complement(23298..24173)
                     /locus_tag="PK242_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOZ92810.1"
                     /translation="MTRPAFLLLTLSTVMLGLASAASAGPLALWRALATLLLACLAHA
                     GGNVLNDYHDARSGADAANRDALSPFTGGARLIQDGQVTERDTARWALWLLLATSAGG
                     VVLASVSGPGLWLIGAAGLVLAWAYSAPPLALMARGLGEAAVAACWWLVVLGADYVQR
                     GALAALPALLGGGLALLVANLLLINGFPDAPADARVGKRTLVVRLGPAAAARLYLGLA
                     LLAHAVVPLAVLAGWAPTGALAGLLSLPLSLVAGGLLWRHRRQAARLRPAIGLTIAAA
                     NLHALALAAGLVLGR"
     gene            24342..24761
                     /locus_tag="PK242_01920"
     CDS             24342..24761
                     /locus_tag="PK242_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOZ92811.1"
                     /translation="MPESDALQSHDEARMRLRRRVATLLWRALALLSLLLGGVGAMLP
                     VLPTVPFLLLAAWAASKGWPALEIWLLNHRRLGPPLVQWRERGAVSRPSKWLSSIMMG
                     GSAIGMQFLPQIPLALRLGAPAVMLAVAIWLWRRPEA"
     gene            24787..25308
                     /locus_tag="PK242_01925"
                     /pseudo
     CDS             24787..25308
                     /locus_tag="PK242_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            25492..25929
                     /locus_tag="PK242_01930"
     CDS             25492..25929
                     /locus_tag="PK242_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOZ92812.1"
                     /translation="MAGAPLAPDEHGVRAAVGYAGDMSPATAWAWVQAGRAALVDVRT
                     DAEREWVGFVPGALAVAWKQWPGMALNPDFDAQMRAAGQGGRPLLLICRSGVRSIAAA
                     RRATELGLTAYNVTEGFEGDPDQHAQRGHLGGWRHAGLPWRQN"
     gene            complement(25957..26643)
                     /locus_tag="PK242_01935"
     CDS             complement(25957..26643)
                     /locus_tag="PK242_01935"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HOZ92813.1"
                     /translation="MSAFQTRCRPMVGFTGDHPMKPSRIASLVCATLVTAAAQAANVN
                     LPGGTLVIPGTSPAGASFTYTGTLTQADTIAFSQTGNPCLQSGNTYCVNGAGVIVVAG
                     TSPVGASTGFGSWTYGALLMQISGVGTVQVWPTNAANGLGSGSPPTGLTLPTTTLAAL
                     GFPAFSQANPTITFTVADTNFGDNSESFTLTQAAAPAAVAPVPTLAEWSLGALALLVG
                     LGGAARARRR"
     gene            complement(26748..28196)
                     /locus_tag="PK242_01940"
     CDS             complement(26748..28196)
                     /locus_tag="PK242_01940"
                     /inference="COORDINATES: protein motif:HMM:NF005686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOZ92814.1"
                     /translation="MHAAAMPTDPTELDASALSAAIHARQLSCRELMAACLARIQRLN
                     PRHNALVSLRPDDELLAEADACDAELARGHSRGWLHGIPQAIKDLAHAAGLPTTLGSP
                     LMKDFIARDDGLMTTRMKAAGCIVIGKSNTPEFGLGSHTFNPVFGTTLNAYDPSKSAG
                     GSSGGAAVALALRLLPVADGSDFMGSLRNPAAWNNLFGLRPSQGRVPMAPAQDVWISQ
                     LGTEGPMARSVRDLARLLATQAGWSPDAPLSIAAGADLASASMPLNLKGLRIGWLADV
                     DAYLPLEAGVLDTCTQALARFERAGSVVEPARLGMSPERVWDAWLVWRRVLVASRIAP
                     FLLKTGNRAHIKPEALWEHDQAQGCSAAEFMAASVTRSAFYQRMRALLARHDVLALPS
                     AQVWPFDARERWPQQIAGRTMDTYHRWMEVVIYASFAGLPCISVPAGFGSKGLPMGLQ
                     LIGQPQGDASLLRVAAAYEALIGDWLATMPPV"
     gene            complement(28307..28789)
                     /gene="lysM"
                     /locus_tag="PK242_01945"
     CDS             complement(28307..28789)
                     /gene="lysM"
                     /locus_tag="PK242_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HOZ92815.1"
                     /translation="MGLFSFIKEAGEKLFGHKEVEQAVATGAPNLAELNQKAADAIKT
                     YVETQNLGLSGLTVGFDGASGKVTLGGNAPSQEAAEKAGLAAGNVASVSDVDNNLQYP
                     AGTASQYHDVVRGDTLSAIAKKYYGDANKYPVIFEANKPMLSHPDKIYPGQKLRIPPL
                     "
     gene            28904..29683
                     /locus_tag="PK242_01950"
     CDS             28904..29683
                     /locus_tag="PK242_01950"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOZ92816.1"
                     /translation="MTFLAIDVGNTRLKWTLYERPAVGARVLAQGVEFLEQLDRLAEG
                     PWADLPEPSRMLGCIVAGQAVKHRVQEQMEHWGVEPRWVVASESEAGLVNGYDFPSRL
                     GADRWVAMVGARHHMLQRGPARPVLLVMVGTAVTVEAIDAQGRFLGGLIIPGHGIMLR
                     ALESGTAGLHVPTGDVVEFPTNTSDALTSGGTFAIAGACERMFQHLFKRCAAEPLCLM
                     TGGAGWKMLPAMSRPYELIESMIFDGLLVIAEAREALNGGL"
     gene            complement(29758..31221)
                     /gene="tldD"
                     /locus_tag="PK242_01955"
     CDS             complement(29758..31221)
                     /gene="tldD"
                     /locus_tag="PK242_01955"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HOZ92817.1"
                     /translation="MNTRNIKHHHLATARALLLDPYGLSERELDATLATIARHRVDDA
                     DLFFQYTRTEGWSLEEGIVKTGSFSIDQGVGVRAVTGERTAFAYSDDLSPGSLQDAAC
                     TVRAISAAAGVGRVDRRRRRASGRNRPLYAPIDPIATLDSGAKVALLEMVEQRARARD
                     PRVVQVMAGLASEYDVVLVARLDGTLAADVRPLVRLSVTVVAEQGGRREIGSSGGGGR
                     FGLTHFDAGRVAQYVDEAVAAALTNLESRPAPAGEMTVVLGPGWPGVLLHEAVGHGLE
                     GDFNRKGSSAFSGRIGQRVAAKGVTVLDDGTLADRRGSLNVDDEGHVTQRNVLIEDGI
                     LRSYLQDTLNARLMKVKPTGNGRRESYAHIPMPRMTNTYMLGGDRDPREIVASIRKGL
                     YATNFGGGQVDITSGKFVFSASEAYWVENGRIQYPVKGATLIGSGPESLKKISMIGND
                     MRLDSGVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTG"
     gene            complement(31341..31453)
                     /gene="rrf"
                     /locus_tag="PK242_01960"
     rRNA            complement(31341..31453)
                     /gene="rrf"
                     /locus_tag="PK242_01960"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         4843 a        11237 c        10837 g         4658 t
ORIGIN      
        1 tcttggtcag cacgatgccg gtgagcggca gcgcttcctt gaaggccctg gcggtattgg
       61 ccgcgtcctg gccctgcatg gcgtcgacca cgaacagggt ctcgacgggg ttcagggcgg
      121 cgtgcagggc cttgatctcc tgcatcagtg cctcgtcgat cgccagccgg ccggcagtat
      181 cgaccagcag cacgtcgtag aagtgctttt tggcgtggtc cagcgcggcg cgggcgatct
      241 cgaccggctg ctgctcgggc gaggacggga accagtcggc gccggcctgc tgcgtcacgg
      301 tcttgagctg ttcgatggcg gcgggacgat agacgtcgcc cgacaccgtc agcaccttct
      361 tcttgcgctt ttgaatcagg tggcgcgcca gcttggcggt ggtggtggtc ttgcccgcgc
      421 cctgcaggcc ggccatcagg atcaccgccg ggggctgcgc ggcgaggttg aggtcgctca
      481 cgccctcgcc catggtggcg gccagctcgc ggttgacgat gcccaccagc gcctggccgg
      541 gcttcaggct gcccagcacc tcctggccca gcgccttgtc cttgacgcgg gcgatgaagt
      601 cgcgcaccac cggcagggcc acgtcggcct ccagcagggc catgcgcacc tcgcgcagca
      661 tgtcctgcac gttggattcg gtgatgcggg cctggccgct gatcttcttg accaggccgg
      721 agagcttgtc ggtgagggcg gtggccattc gagcggttcg cgccgcacag ggcgcgccaa
      781 aagcgtgtga aaacccgcgg cggccggccc gtccgcgctg gacgagaggg gcaagccgct
      841 aaactttgcc catgattcta gccagcgcct ctcccgtcag cctggggctc gccgccgccg
      901 ccgccctggc ctacgcggtg ccggcgctgg ccgcacgcgc cctgggcgcc gcaggcgcgc
      961 ggcgcgtgct gctgctggcc tggctgctgc acggcgcaca actggcctgg ggcgtgctgg
     1021 gcagcgaacc gcgcttcggc tttgcaccgg cgctgtccat caccgtatgg ctggtgctga
     1081 ccgtgtacgc cattgaaagc cagctctacc cgcagatgac cgcgcgctgg gcactggccg
     1141 gcttcggcac gctggcgatc ctgctggcgc tgctgtttcc gggcgcccat ccgccggcca
     1201 ccggctcgcc cctgctgccg ctgcactggg ccctgggcat tgccgcctac ggcctgctgg
     1261 ccgccgccgt ggcccatggc tggctgatgc agcgcgccga agaccagatg cgccacgcca
     1321 cgcagacggt cgacgggggc gtgccgctgc tgacgctgga gcgcctgatg ttccgctttc
     1381 tgggcgccgg cttcgtgttg ctgacgctga ccatgctggc cggcgccttg ttcggcgagc
     1441 agctctacgg cgccagctac accggctggc gctgggacca caagcgggtg ttcaccctgt
     1501 tgtcctggct gaccttcggc acgctgctgg ccgggcgctg gtggttcggc tggcgcgggc
     1561 ggcgtgcggt gcgcgtgctg tacatcggcg cggcgctgct gctgctgggc tacgtcggct
     1621 cgcgcttcgt gctggaagtg ctgctggggc gggtcgcgtg aagttgctga tcgtgctgct
     1681 ggccgtgctg gcggccgtct ggctgtggcg gcgtggacgc cggccgtcgg ccccaccacc
     1741 gtccgaccgg cgcggccggg ggcaggccct gcccatggtg cgctgtgacc gctgcggcgt
     1801 gcacctgccg ggggcggacg cggtgcgcgg ccaccggggc agctactgtt gcgtggcgca
     1861 ccgccgcgac gccgaaggcg cctgatgtcg tcccgccgcg cgcacgagca ggccacctgg
     1921 ttcggcaccc agctgccgac ggcgccgtca ctgccggccc tgaccctgag ggccgacgcc
     1981 gatccctcgc ccggctttgc ccggctgtgg tcggccttca tgtcggcccg ggtgctgatc
     2041 gcgctggcgc tgctgggtct gcagctggtg ctgcatgtgc tggcacgacc gggctcgccc
     2101 tggctgctgg gcaccagcgt cgcctacgcg ctgttcacca tcgccgtgcg ggtgatgggt
     2161 gagccgcgcg gccccgggcg tgccttcgac agccagtggc tgcccacggt gggggtcgat
     2221 ctgctgtttt tctcggcgct gcagttgcag cccaccccgt cgcccatcaa ctacacgccc
     2281 ttgctggcgc tgccggtgct gatggccgcc atcatgggca cgcgcgccct ggcgctgggc
     2341 acggcggcgc tggtcacgct gatgctgctg ggccacgcag cgtgggcggc cagcggctcg
     2401 tcctgggcca acaccgccga gctgttgcag gcgggcctga ccggcgccgg cctgctggtg
     2461 ctggcgctgc tgaccaacca gatggccatg cggctggcac gcgaggaagc gctggcgcgc
     2521 cgcaaccggg tcgaggccca tgtgcaggcg atggtcaacg ccatggtgat cgaagccctg
     2581 cccgacggcg tgctggtgat cgacaccgag catctggtgc gggcggccaa cccggcggcg
     2641 cggctgatgc tggggtcgga cctggacgtg acgccgccgg tgttcagcct ggaggacaac
     2701 cccgcctggg gacagctcaa gcgcatcgtc cgtcagacct tcagtgacgc gccggtcgat
     2761 gccaccgaag tgaccctgca ccatgaagat cgcatgagca gccacctgca ggtgcgcacc
     2821 cagcgcacac catcgatcgg cgacagtggc gtcagcctgt gcgtgatgtt cctgcaggac
     2881 ctgcgcgagc tggaagcgcg cctgcgcacc gagaaactgg cgtcgatggg ccgcatgtcg
     2941 gccgccgtgg cgcacgagat ccgcaacccg ctggcggcca tcagccaggc caacgccctg
     3001 ctcgacgagg atctgcacga accgttgcag aaacgtctgg ccgacatggt gcggcagaac
     3061 gccgagcgcc tgcagcacat cgtggacgac gtgctggatg tcgcccgggt gcagggccag
     3121 ggcggcgagt tcagcccggt gccggcgctg gcgctcgacg ccgaggtgga agacttttgc
     3181 aacgaatggg cggcccagca tggtcaggtg cagcggctgc tgctgcggct gggcgccggc
     3241 gacgcccacg tgcagttcgc gcccgagcac ctgcggcgcg tgctggtcaa cctgctggac
     3301 aacgctgcgc gctacgcgtc gcgccgcgcc ggcgccatcc aggtggagac gctggcggtg
     3361 cggcacggcc cggtacggct gatggtctgg agcgacggcc cggccatgga ggcggcggtg
     3421 cagcgccacc tgttcgaacc ctttttctcg tccgaaagcc gctccagcgg cctgggcctg
     3481 tacatctgcc gcgaactgtg cgagcgccac ggcgccagca tcgcccacga gcgcaacgcg
     3541 cgcgctcagg gcgggcaaag ccacgagggc aacgaattct tcatcagctt ccggcgtgcc
     3601 cacagcggac actcgacttt ctgattctgg agctctgcgc gcagccatga acgcttccgt
     3661 gaccatgccc ggcgccgcca gcatcctggt ggtggacgac gaacccgacc tgcgcaccct
     3721 gtacgagctg acgctgctgc gcgagggcca tcgggtgagc tcagcggcgt cgctggccga
     3781 ggcacgggcg ctgctggcca cccaggccta cgacctggtc gtcaccgaca tgcgcctgac
     3841 cgacggcctg gggctggaac tgctgcgcga gatggcggcg gcccagcggc gcgaacgcag
     3901 catcgtcatc accgcctacg gctcggccga gaacgcggtc gaagccctca agtgcggcgc
     3961 cttcgactac ctgaccaagc cgatcgacct caaacagttc cgcggcgtgg tcggcgcggc
     4021 gctgcaagcc cggagcgcca ccccggccga cgacgccgcg cccgcgcgcg aaccggcccc
     4081 accgaccccg gctgccacgc cggcgcccgc cgcgcccgct gccgccgcca gcggccccgg
     4141 cgccgttgcg ctggcgaggc tggtggggga atcggccgcc atgcgcgcgg tcaagcagcg
     4201 catcgccaag gtggcccgca gcatggcgcc ggtgctggtg tgcggcgaat cgggcaccgg
     4261 caaggagctg gtggcgcgcg ccatccacgc ctgcagccac cgcagcgagg gccccatggt
     4321 gccggtgaac tgcggcgcca tcccggagaa cctgctggag gccgagttct tcggcgcccg
     4381 caagggctcc tacaccggcg ccacgcagga ccgcgtgggc ttctttcagg cagcgcgcgg
     4441 cggcacgctg tttctcgacg agatcggcga gctgccgctg gccatgcagg ccaagctgct
     4501 gcgcgccatc caggagcggc gcgtgcgtcc gctgggcgaa acccaggaag agccggtcga
     4561 cgtacgcatc gtcagcgcca cccacaagga cctggcggcc gaggtgcagg ccgggcgctt
     4621 ccggcaggac ctgtactacc gcctgaacgt gatcgaaatc gtggtgccgc ccctgcgcga
     4681 gcggcgcgaa gacctgccgg ccctgtgcca gaccctgctg gggcgcatcg ccgccgatgc
     4741 cggcatcgcc gtacccacgt tgtcggcacg ccagttgcag gacatccgca ccgccccgct
     4801 ggagggcaac gtgcgcgaac tggaaaacct gctgcaccgc gccgtcgccc tgggcgaagc
     4861 cggcgcttcc gggttcgggg agcttgaatc cggcacccca tcaccgcagg cagctaccga
     4921 ttcaatagcg atctcgggtc ccacggtggc gccacccgac gccccccgga cgccgccgca
     4981 caccgacctg cccagcaacc tggaaagctg gctggacgcg cgcgagcgcg aggtgctggt
     5041 gcgcgcgctc gacgaaaccg gcttcaaccg caccgccgcc gcggcccggc tgggcctgaa
     5101 cctgcgccag atgcgctacc gcatggcgcg cctgggcatc accgccccga gcgacgacga
     5161 accaggcccc gatgaaggca agtgaaccca gcgcaagcgc gccgcgccgc gccgcctggc
     5221 agggtggctg gtaccgggcg gcgcgcgccc tgccctcgcc caaccacggc ccacgcccgg
     5281 cgggcagcga ggtcgacctg atcgtcgtgc actcgatcag cctgccgccc ggcgagtacg
     5341 gcggcgacca ggtgcaggcc ctgttcacca acacgctgga ctgggacgcg cacccctact
     5401 tccagccgtt gcgggggttg caggtgtcgg cgcatttcta cgtgcggcgc gatggtcggc
     5461 tgtggcagtt cgtcagctgc gacgcgcgcg cctggcatgc cggggtgtcg tgctttcgcg
     5521 ggcgcgagaa ctgcaacgac tactcggtgg gcgtcgagct cgaaggcctg gaaggcggac
     5581 ccttcgagcc ggcgcagtac gccacgctgg cccggctgtg ccgcgcgctg gcgcggcgct
     5641 acccgatcga ggccatcgcc gggcaccagc acatcgcgcc cggccgcaag cacgaccccg
     5701 ggccaggctt cgactggcgg cacctgcaag gcctgctgcg ctggagcgcc aggcgctttg
     5761 ccgacggcgc cctggggtaa tccacgagtt cggcgccgcc gcgcccgcat cgaccgggca
     5821 ccgcgccttg atccatgcgc cctggcggcg caaatcctgc gacagaccgc gcccctgcgt
     5881 ggcgcgcgcc cgcccacccg cgccgagcct gacggcgcgc ccgctgcggg cctcgggtgc
     5941 tccggcacca ggctacgcgt ttgcccgcac acactacccc ttgtgcattg aacgattagc
     6001 aaacacaaga tatagtgtcc gtcgtcgcgg ccacctggcg ccggccagac cggcgcgtca
     6061 gcgcgtcgcg catcgacatt ccgccaccca tccatcgagg accccatgca gacaccgcac
     6121 accctcccgg ccagcgtcgc cggccccccg ggcacactcg cgcacgaccg cgccggcaca
     6181 ccgaccgcga ccacgcagac gctgacgcac taccagatca tccggcgcaa cggggccgtg
     6241 gtcgggttcg agcccgccaa gatcgcggtg gccatgatga aggccttcct ggccgtgcac
     6301 ggcacacccg gcgcggcttc ggccagcgtg cgcgaaacgg tggagcaact gacgcagacc
     6361 gtggtgcgcg ccctgctgcg ttcgcgcccg gccggcggca ccttccacat cgaggacgtg
     6421 caggaccagg tcgagctggg cctgatgcgc ggcggccacc acgaggtggc gcgcgcctac
     6481 gtgctctacc gcgaacgccg cgcgcaggag cgggcgcgcc aggcggccgg cagtcagccg
     6541 gccgcgccgg tgctgcacgt gctgcaaaaa ggggagcttg tggcgctgga tgtgcctgcg
     6601 ctggaggccc ttttgaatca ggcctgcgcc aacctggacg gcgccgtgca gagcgccccc
     6661 atcctggccg aaaccctgcg caacctgtac gacggcgtgc cggtcgagga ggtctacaag
     6721 gccgccatcc tggccgcgcg cacgctgatc gagaaagagc ccgactacag cttcgccacg
     6781 gcgcgcctgc tgctgcacac catcgccgcc gaggtgctgg gcgaggcggt ggacttgccg
     6841 acgctggcca gccgctacgc cggcaacttc cccgacttca tccgccaggg cgtggccggc
     6901 gagctgctcg acgagcgcct gctgcagttc gacctcgcgc gcctgggcgc ggcgctcaag
     6961 cccgagcgcg acctgcagtt cgactacctg ggcctgcaga cgctgtacga ccgctacttc
     7021 ctgcacctga acaagcgccg catcgagctg ccgcaggcct tcttcatgcg ggtggccatg
     7081 ggtctggcgc tgaacgagat cgaccgcgag gcgcgcgcca tcgagttcta cgagctgctg
     7141 tcgtcgttcg acttcatgag cagcacgccg acgctgttca acagcggcac gcggcgcccg
     7201 cagctgtcga gctgctacct gaccacggtg cccgacgagc tggagggcat ctacgagtcg
     7261 atcaaggaaa acgccctgct gtccaagcac gccggcggcc tgggcaacga ctggacgcgc
     7321 gtgcgcgcac tgggcagcca catcaagggc accaacggcg aatcgcaggg ggtggtgccg
     7381 tttctgaaag tggccaacga caccgcggtg gccgtcaacc agggcggcaa gcgcaagggg
     7441 gctgtgtgcg cctacctgga aacctggcat ctggacatcg aggagtttct ggagctgcgc
     7501 aagaacaccg gcgacgaccg ccgccgcacg cacgacatga acaccgccaa ctgggtgcct
     7561 gatctgttca tgcgccgggt ggtggaaggc ggcacgtgga cgctgttctc gccggtggac
     7621 gtgcccgacc tgcacgagct gtgcggcgcc gccttcgagc aggcctacgt cgcctacgaa
     7681 gcgcgcgcgg cgcgcggcga gatcagcccc agcaggacgc tgccggcggt cgatctgtgg
     7741 cgcaagatgc tgtcgatgct gttcgagacc ggccacccct ggatcacctt caaggacgcc
     7801 tgcaacctgc gcagcccgca gcagcacgtc ggggtggtgc attccagcaa cctgtgcacc
     7861 gaaatcacgc tcaacaccag cgacagcgaa accgcggtgt gcaacctggg ttcggtcaac
     7921 ctggcacagc acctgcgcga cggtcgcatc gatcacgaca agctcaggaa gacggtttcg
     7981 acggccatgc gcatgctcga caacgtcatc gacatcaact actacgccgt caagaaggcg
     8041 cgcgacgcca acctgcgcca tcgcccggtc ggtctgggca tcatgggctt tcaggacgcg
     8101 ctctacgcgc tgcgcctgcc ctacgccagc gacgcggcgg tgcagttcgc cgacgaatcg
     8161 atggaagcgg tctgctacca cgcctactgg gcttcaaccg aactggcggc cgagcgcggg
     8221 cgctacagca gctaccgcgg ctcgctgtgg gatcagggcg tgctgccgct cgacagcctg
     8281 ggattgctgg agcgcgcgcg cggcggccac gtcgaggtgg accgcagcag cacgctggac
     8341 tgggacgcac tgcgcgccaa gatggcgcgc gacggcatgc gcaacgccaa ctgcgtggcc
     8401 attgcgccca ccgccaccat cagcaacatc gtcggcgtcg acgcctcgat cgagccgagc
     8461 ttcggcaacc tgtcggtcaa gagcaacctg tcgggcgaat tcaccgtcat caaccagcac
     8521 ctggtgcgcg agctcaagcg cctggatctg tgggacgagg tgatggtgat ggacctcaag
     8581 cacttcgacg gttcgctgcg cgcgatcgac cgcgtgccgg ccgacatccg ccagctctac
     8641 gccaccgcgt tcgagatcga gccgcactgg ctggtcgaag cggcggcgcg gcgtcagaag
     8701 tggatcgatc aggcgcagag cctgaacctg tacatggccg gcgcctcggg ccgcaagctc
     8761 gacgaaacct acaagctggc ctggcggcgt ggcctcaaga ccacctacta cctgcgcacc
     8821 accagcgcca cgcaggccga gaagtcgacc atcacctcgg gtcgcctgaa cgccgtgccg
     8881 catggcgatg acgaggcgct ggccatgcgc gcgcgcgccg cgccgaccac cgacgtggcc
     8941 gcgaccgttg ctgcgcagcc cgccaccgac atccgcttct gcgccatcga cgacccgggc
     9001 tgcgaagcct gtcaatgaag cacccatcgc tgcgaggccg cgttcaacga tgcctcgctg
     9061 caacacacct accccatcca tcgggccgca gcgtgacgga atgctttgca tttcgtggcg
     9121 ttgctcccat aatcggagca ttggacaaat catgttgacc tgggatcacg agaccactct
     9181 caccacgccg cagccgcgcc tgcagccggt gaccgagtcc gcgccttcca ccacccgcgc
     9241 caccgacgca cacagcggcg tcgccacggg cagcccgcgc cgcgtcaacg ccgccgacaa
     9301 gcgcatcatc aacggccaga ccgacgtcaa ccagctggtg ccgttcaagt acaagtgggc
     9361 ctgggaaaag tacctggcca cctgcgccaa ccactggatg ccgcaggaaa tcaacatgaa
     9421 ccgcgacatc gcgctgtgga aagacccgaa cggtctgacc gaagacgagc gccgcatcgt
     9481 caagcgcaac ctcggcttct tcgtcaccgc cgactcgctg gccgccaaca acatcgtgct
     9541 gggcacctac cgccacgtca cggcgcccga gtgccggcag ttcctgctgc gccaggcctt
     9601 cgaggaagcc atccacaccc acgcctacca gtacatcgtc gaatcgctgg ggctggacga
     9661 aggcgagatc ttcaacgcct accacgagat cccgtcgatc cgcgacaagg acgaattcct
     9721 gatcccgttc atcgacgcga tcatggaccc cgatttccgc accggcacgc ccgaggccga
     9781 ccagcgcctg ctgaagtcgc tgatcgtctt tgcgtgcctg atggaaggcc tgttcttcta
     9841 cgtcggcttc acgcagatcc tcgcgctggg ccggcagaac aagatggtcg gcgcggccga
     9901 gcagtaccag tacatcctgc gcgacgagtc gatgcactgc aacttcggca tcgacctgat
     9961 caaccagatc aagctggaaa acccccacct gtggacgccg gccttcaagg ccgaggtgcg
    10021 cgccctgttc gagcacgcgg tcgagctgga gtaccgctac gccgaagaca ccatgccgcg
    10081 cggcgtgctg ggcatgaacg cctccatgtt caagggctac ctgcgccaca ttgccaatcg
    10141 gcgcgccacc cagatcggtc tggacgcgct gtaccccaac gccgagaacc cgttcccctg
    10201 gatgagcgag atgatcgatc tgaaaaagga acgcaacttc ttcgaaacgc gcgtcatcga
    10261 gtaccagacc ggcggcgcgc tgtcgtggga ctgacccccg ctgaaccgaa tgtgcacccg
    10321 attcgccccg accgccgaca gcctgctgtc gtcggttcgg tgcgcccctg ttcatggtgt
    10381 tggggcctgc cccagcacca tgaatcgctt cgcacgcgcc aacccgcgtg aagcgtttcc
    10441 tgctgttgaa cgttcaactt gatcgaggag aaaaaaatgg caactgcgaa gaaagcacct
    10501 gcgaagaagg ccccggcgaa gaaagccgct ccggccaaga aggccccggc gaagaaagcc
    10561 gctccggcca agaaagccgc tccggccaag aaagcggcac cggccaagaa ggccgcgccc
    10621 gccaagaagg caccggcgaa gaaagctgcg ccggccaaga aggccgcacc ggcgaagaaa
    10681 gccgcacccg ccaaaaaggt agccgccaag aaagcggcac cggccaagaa ggccgcaccg
    10741 gcgaagaaag ccgcgcccgc caaaaaggta gccgccaaga aagcggcacc ggccaagaag
    10801 gccgcgcccg ccaagaagcc ggctgccaag aaagccgcgc cagccaagaa agcagcggcc
    10861 aagaagccgg ctgcgcaaac cacgctgagc ccgcaggcag cgtggccgtt tccgacggcc
    10921 agcaagccct gaccggtgat ggcgcacccg gccctgaacg gccgggtcgg ttcaccatgg
    10981 cccgccacgg cccagccgtt ggcgggcttt ttcctttctg cgcgcgggtg caggccgcca
    11041 ccttcagcct ggtgcagtgt ttcgggctca gggcagttcg gcgctgcgca tgcgcgccaa
    11101 gatggtgcgg gtgcggctgg ccaggtaggc cgaacgcggc agcttctcga aatagcgcgg
    11161 gcgcggcagc atgacggcca gccgggccga ctccgcggcc gtcagttgcg ccgcgctctt
    11221 gcggaagtag tgctgcgccg ccgcttcggc gccgaacacg ccctcgcccc actccacatg
    11281 gttgaggtag agctccagga tgcggcgctt gtccagcagc tgctccagtg cgaaggtgag
    11341 caccagctcc tgccccttgc gccacaggct gcgctcgccc gacagcagca ggttcttggc
    11401 cagctgctgg gtgatggtcg agccgccacg gatgcggatc gggcgcgccg gccggttctg
    11461 cgccgcgcgc tcggccgcgg cttcctcggc ggcggcgttg cgctgccagg ccttttcgat
    11521 ggcgtcccag tccaccccct cgtggttggc aaagccgtcg tcctcggacg cgatgacggc
    11581 gcgcttgagg tgatcgctga tctgcgcgta gtcgcgccac tgctggcgcc agcgcagcgt
    11641 gccggtgctg gccaggcgcc aggcttcgga acgctcgaag ctggtggagc gcgggtcgag
    11701 caccaccatc agcgccacgc ggccgacgaa gaacagctgc aacagcagca ggcccagcac
    11761 ggccagcagc aggatgcggc gcagcacagc ccccatggtc gatgcccgtc ccgccgtcag
    11821 accacgcccg gcggtcggtg ggtggcgggg cgcgggtggt tcatttcaac tcccgcaccg
    11881 aggtcgccag cgggtcggcc tgcagcatgg tctccagggt ctgctcgcgc gtgaacctga
    11941 agcgcgcggt gacgtcgaac tggtcggtgt ccttgcgcat ggcggcggtg aagttgccga
    12001 acggggcggc gcgcgccgcg atcagctcgg ccagcttgtc cagcgcacgc tggcccgagc
    12061 cgcgcaccac ccgggcgtcg agaatgcggc catcgtggtt gatcagcagc gccatgatca
    12121 gctcgccata cagcttacgc ccagccatct gcggaaagtt ggcggtgccc tcggcctcga
    12181 tcttgcgccg catgctgtcg tagtagaccg cgtaggtggt gcccagcgtg gcggggctga
    12241 ggtagcgctt gcgcgggcgc gagttctctt cctcgacccg cttttcgatc gccgccagca
    12301 ggcgcgccag ttgctggcga cgctcttcct gggcgcgcgc ctcgggatct tcgctcagct
    12361 ggcgcgggtc gggcttgggc atggccgcca gttgctggcg cacctgggcc agcagctgct
    12421 cctgctgctg cagcatggcg tcgatgcggc gctggttctc ggtgaccggc gcatcgcccg
    12481 gcgcttcgtg ggggctgggc ggcagcggcg tgctggccat gcgccgatcg gccgcttcgc
    12541 cgccaccggc caggctggac tgggcgatgg cctgggcctt gacgggcttc tcgtccgatg
    12601 cggcgttgac cagcaccacg tcgagcgtgg tctcctgaaa caggcgcttg aagccctcgg
    12661 gatcgacgaa acgcaccgtc agcagcaccg cgtgcagcag caccgacagg ccgagcgccc
    12721 attgcagcgt ggacagcgtt ttatacgaac gaagcattac gagggcggat tatcggtgcg
    12781 cgcgccttcg gtttcgtcca gctccaccgc aatggcgatc ggcccggcgg cggcgccctc
    12841 gtcgtcgtcc tcggcctcgg cggctgtggg caccgccgcc tggtcctgca acacctcgag
    12901 gacggtgccg tgcacgtcga gcgcgatgtc gtcgagctgg cccagccgca cccgcacccg
    12961 ggtgccacgc ggcaggccct gcgcgcccat cacggtaaac accagcggca gctcgtcggc
    13021 gcgcaccagc caggcgccgc cggtctgctc gcgcagcagg ctggcgttca gctcggtgat
    13081 gccttgctgg tgcaggtagc gcagcgtcca gaagcgttcc atgctggcct ggtgcgcgcc
    13141 ataggcgctg taggcggcat cgaaggcgct gatgatggcc agcagcgccg cgtccttggg
    13201 cttgaacggc gccaccagcg ccgccgccgt gccgtggcgt gcggcggcga tgatctgcca
    13261 ctggttgacc aggtcgacgt agcggcgcag cggcgaggtg ctccaggcgt agctgggcac
    13321 acccaggccg gcgtgcggca gcgcccgcgc gcccatgcgc accttgatgc cgggcagcag
    13381 gctggcctgg ctgcggtaga tggccggcac gcccagggcc ttgagccatt cgccccagct
    13441 gctgttggcc aggatcatga gttcggcgac gatcagatcc agcggcgcgc cgcgcgagcg
    13501 ggtggtgatc tgcacctgct cggcgccggt gggcacggcg ccgtcgttgc cgaccaggcg
    13561 gaagctgtag tccggccgcg tgaaggtttc cggcttgccg cgcacctgct cgcgccgggc
    13621 cttcaggtgc cggctcagtt gatataaaaa tgatagctgt tcgcgctcga caggcaagcg
    13681 ctgggggtcg atttgaccat caaccgaagc gccttgcagc cagtcctcgg tgatcagcgc
    13741 gtcgagctgg tcgtggcgca ggttggcggc gatcggcacc cgctccagcc gcgtctggcg
    13801 gtcctggatc tccagcgtgg cttcgtccag cgtgacatac agcgacaccg ccggcaccgg
    13861 cgcgccttcg gcgagcgtgt aggcgtcgac cacctcgtcg ggcagcatgg tgatcttgta
    13921 gcccggcatg tagacggtgg acaggcgcgc gcgcgccacg tcgtccagcg cgtcgcccgg
    13981 ctgaatggcc aggccgggcg ccgcgatgtg gatgcccagc accaccgtgc cggtgcccag
    14041 cccctgcagc gacagcgcgt cgtcgatttc ggtggtggcc gagtcgtcga tcgaaaacgc
    14101 cgccaccggc gccagcggca gctcctcgcg gatgaccggc gcggccagcc gggcaaaacc
    14161 cgtgccctgc gggaagttct cgaacagaaa gcgctgccag tggaactggt aggccgactc
    14221 gatggcgccg gcgcgctgca gcagcgccag cggcgccagc tgggtggcgc ggctggcctc
    14281 gaccacggcc ttgtattcgg gggcgttgcg gtccgggcgg aacaggatgc ggtagagctg
    14341 ctcgcgcacc ggcgccgggc actggccctg cgccagcgcg tcggcccagg cggcgatctg
    14401 ggccgcgagc tgctttttct tctcgatcgc cgccagcgcc tgcgccacga cgtcggccgg
    14461 cgccttgcga aagcgcccct tgccggcgcg gcggaagtag tgcggcgcct cgtgcagcgc
    14521 gatcagcgcc gccgcctgct gcaccgggcc ggggccgccg ccgaagtagt cggccgccag
    14581 ctcggcaaac ccgaactcgt cttccggcgc gaattcccag gccagggccg ggtcgacgtc
    14641 ggcggcctgg gcctgcgcct gggccagcag ctcggccggc gccggcttgt cgaagcgcag
    14701 caactggttg gcggccttga ccttgacgcg cttgccggag tccagctcca cctgcgccga
    14761 ggcctcggcc tcggaaagaa tgcggccggc aagaaacttg ccggcctcgt cgaacagtac
    14821 atgcatggga cgccgattgt cccactccct gccgcgccgg cgctcagccg gccatgcgcg
    14881 ccagcacggg cgtgtggccg gcgcgctcgt gccacagcgc cgccgccacg tggcccagca
    14941 ccagggccag caaggcccaa cccaggttgc cgtgcagcag ccggcccagc tgcagcgtcc
    15001 attccacctt ctggctgacg tcgaagcccg gcagcagcgg caggccgaac ggcgcaaacg
    15061 cgcgtcccga gccccactgg cgcagcagcc cgatggccgg cacggccagc atcagggcgt
    15121 acaggcccag gtgccccaga cgggccagcg cgctcaccgc cggcggccgg cggcgccgcg
    15181 ccagtgccca ggcgacacgc gccagcagca gggccagcag cagggtgccg accggtttgt
    15241 gggtgggcca cagcagcgcg tccagcgccg agcgctcggc cagcgcatgc gcgccggcgg
    15301 agacgaagat ccaggcgaac agcccggcca tgccccagtg cagccagcgg ctgatgcggt
    15361 cgtagcggac gggggtgggg gtgggggtca tcgtgcaagc ctttccgggg ttcgggtgcc
    15421 ggtgatgctg tcacccgccg gtgaagcccg tccgtctgcc gtgtcaagcg gttgtaaagg
    15481 cctttggcgc atgcccaaac tgcgccggaa gctatagtta cgatagcgtt acagaaggtc
    15541 cagaaagctc agcacggcgt cgatgtggtg ctcgaagtcg ctcagcgcgt ggtcgctgcc
    15601 ctcgatcagc cgcagcgccg cgccggcgta gcgcgccgcc atctcgcgcc agtccagcag
    15661 ctcgtcgccc tgggcgatga cggccagaaa gcgctcgggc gcctgcagcg ggccgggtgc
    15721 cagggcgcgc agctcgtcca cgtattcggg gcggaacaag aagcgctcgg ccgggtcgtg
    15781 ccaggcggtc tgctcgccga tgtgcgccgc caggtcgcgc gccggatcga ccgccgggtt
    15841 gagcaccacc gcccggcagc cgcgccgctg ggccagccag gtggcgtagt aaccgcccag
    15901 cgaggagccc accaccgcca tgtgcgcggc cggccagtcg gcgctgccct gcgcgatcaa
    15961 cgccgcggcc tcgcggggcg agggcggcag ctgcgggcac caccaggtca cgcccgggtg
    16021 gtcggcgcgc acgcgcgccg ccatctggcg cgccttggcc gattgcggcg aggagcgaaa
    16081 tccgtgcagg tacagcaaat gggtggtcgg catgcgggca atgctatggc tttggaagcc
    16141 tgacatttga acccaatcca taccggcgcc gcaggctgcg ctgcggggcg gcgggataat
    16201 ggcgccatgg ccgtggtgtt cgacaaaccg tcgctctggc agcgtctgtg gccgctgctg
    16261 cgcggcttcg acctgctgct gctggtggcg gtgctggtgc tggccggcat gggtctgatg
    16321 accatgtatt cctccggctt cgaccacggt acgcgctttg ccgaccacgc ccgcaacatg
    16381 ctgatcgccg ccggcatcat gttcgtggtg gcgcaggtgc cgccgcagcg gctgatgagc
    16441 ctggcggtgc cggtctacac cctgggcgtg ggcctgctgg tggcggtggc gctgttcggc
    16501 atcaccaaga agggggcgca gcgctggatc aacgtcggcg tggtgatcca gcccagcgag
    16561 ctgctgaaga tcgccacccc gctgatgctg gcctggtggt ttcaccgccg cgagggccag
    16621 acgcgcccgg tcgacttcct ggtggcgttc gcgctgctgg ctgcgcccgt ggggctgatc
    16681 atgaagcagc ccgacctggg caccgccctg ctggtgggcg ccgccgggct ggcggtgatc
    16741 ttctttgccg gcctgccctg gaagctggtg ctgccgccgg tgctgctggg cgcgctgggc
    16801 gtgggcctgc tgctggccta cgagggcagc ctgtgcgccg acggcgtgga ctggcgcgtg
    16861 ctgcacgaat accagcgcca gcgcgtgtgc accctgctcg accccacgcg cgaccccctg
    16921 ggcaagggct ttcacacgct gcagggcatg atcgccatcg gctcgggcgg gctgtggggc
    16981 aagggcttca tgcagggcac gcagacgcac ctggagttca ttcccgagcg caccaccgac
    17041 ttcatcttcg ccgccttctc ggaggaattc ggcctggctg gcaacctggc gctgatcgcc
    17101 ggcttcgtgt ttctgatcct gcgcgggctg atgatcgccg ccgaggggcc gacgctgttc
    17161 gcccgcctgc tggcggcggc gctgaccacc atcttctttg cctacgcctt cgtcaacatg
    17221 ggcatggtca gcggcatgct gccggtggtg ggcgtgccgc tgcccttcat cagctacggc
    17281 ggcacggcca tggtcacgct cgggctggcg ctgggcatgc tgatgtcgat cgccaaggcg
    17341 cggcgcaccc tgatgcagag ttgacgccac gtcggtgtgc ggcattgcac gccggcgtgc
    17401 cccgaagtcg cccggacggc ccctcttggc cccacaatgc cgggcagccg ggctgggccc
    17461 ggcgcatcca ccccccatca ctcaaggagc gacattcatg gcaggcaagt tcgaactcaa
    17521 gaagtccaag aacggcaagt tcttcttcaa cctgctggcc agcaatggtc aggtcatcct
    17581 cagcagcgag atgtacgagg ccaaggccag cgcggtcaac ggcatcgaat cggtgaagaa
    17641 aaacggcgcc gacgccgcgc actacgaaaa actgaccggc aaggacggct cgccctactt
    17701 cacgctcaag gccggcaacg gccaggtgat cggccagagc cagatgtatg ccagcgagac
    17761 gacgcgcgac aacggcatcc agtcctgcgt cagccatgcc cccggcgcca cggtcgacga
    17821 ccagaccgcc caggcctgac cgggtcagcc ccagccagcc agccgcctgc gggcggcttt
    17881 tttgcgtccg gcacgccgcg gcggccttgt tttcagtggt gttttttgcc gctacggcgt
    17941 acccaccaag cgccagcaac tattgtaatc atagcgttct gacggatatg caaggccctg
    18001 cgccaacggc tagactgggg ccatgaaact gctcgaaccc atcctggcgc aggccgcggc
    18061 catctcccaa ctgcggcgcg acatccacgc ccaccccgag ctgtgcttcg aagaggtgcg
    18121 caccgccgac ctggtggcgg ccaagctggc cgagtggggt attgccaccc accgcggcat
    18181 gggcaccacc ggtgtggtcg gcatcgtgca cgggcgtgac ggcggcaagt cgggccgcgc
    18241 catcggcctg cgtgccgaca tggacgcgct gccgatgacc gaattcaaca ccttcgccca
    18301 cgccagccgc caccacggca agatgcatgc ctgcggccac gacggccatg tggccatgct
    18361 gctggccgcc ggtcagcagc tggcggccac gcgcgacttc gatggcacgg tgtacctgat
    18421 cttccagccc gccgaagaag gcggcggcgg cgcgcgcgaa atggtcaagg acggcctgtt
    18481 cgaccgcttc ccgatggaag cggtgttcgg catgcacaac tggccgggca tggaagccgg
    18541 caggtttgcg gtctcggccg gcccggtgat gggctccagc aacgaattcc gcatcgtcat
    18601 caccggcaag ggcggccacg cggccatgcc ccacaccggc atcgacccgc tgccgatcgc
    18661 ctgccaggtg gtgctgggtt ttcagaccat catctcacgc cacaagaagc cggtggatcc
    18721 gggcgtcatc tcggtcacca tggtccacgc cggcgaggcc accaacgtgg tgcccgacgc
    18781 gtgcgagatc cagggcacgg tgcgcacctt cagcttcgag acactcgatc tgatcgagca
    18841 gcgcatgcag gccatcgccg aaggggtgag cgccgccttc ggcgcccagt gcacgttcga
    18901 attcacgcgc aactacccgc ccaccatcaa caccgcctac gaggccgagt tctgccgccg
    18961 cgtgatggcc ggcatcgtcg gcgaagccaa cgccgtaccg caggaaccca ccctgggcgc
    19021 cgaggacttc gcctacatgc tgctggaaaa acccggcgcc tactgcttca tcggcaacgg
    19081 catgggcgcg caccgcgggc gctacgaagg cggtggccat gacgccggcc cctgcaccct
    19141 gcacaacccg cactacgact tcaacgacga tctgatcccg ctgggcggca cctattgggt
    19201 gcagctggcg cagcagtggc tggcgcacca gcccccggcc gaccgcccgc tgccacgcaa
    19261 gggctgagcc ggtgtgagcg aacctttcat gcccgctgcg accgccgcaa cgcagccgcc
    19321 cgacgtggcc cgtggccgcc gtggcgcagg cgccggcggc gcgcggcgcc ctgatcggcc
    19381 gcgtcgcggc gtacccctgg cgtgcccgac atgcatgccc agctgctgag cctgccgcca
    19441 tgatcccgat cccgcaggcc tttgccgcca gctacgccca ggcgcgcgtc aagttcctcg
    19501 aagcggcggc cagcgccagc acgcagatcg agagccaccg ccatcccctg cccggccgcg
    19561 acggcgagtt gctggcgctg gacctggcgc tcgatggtga cgcgcagagt gagcgcctgc
    19621 tcatcgtcag cagcgcctgc cacggggtcg agggcttttg cggctcgggg gtgcaggtgt
    19681 ttgccctgca cgacgccgac tggcgccagc aggcgcgcga cgccggtgtg gcggtgctct
    19741 acctgcatgc gctcaacccc tggggcttct cgcacctgcg gcgcgtgacg cacgaagggg
    19801 tggacctgaa ccgcaacttc cccgacttca gccagccgct gccggtcaac accgcctatc
    19861 gccagctgca tccgtggctg ctgccgcccc agtggccgcc cgacgccgcc caccgcgccg
    19921 cggtgcagga cttcatcgcg cgcgagggcg tggccgcctg gcagaccgcc atctcgggcg
    19981 gccagtacga attccccacc ggcctgttct acggcggcca cgcgccgacc tggagccacc
    20041 agaccctgcg cggcgtgctg gcgcggcgcg ccggacgcgc gcgccgcgtg gcctggatcg
    20101 acctgcacac cggcctgggc ccgccgggcg tgggcgagcg catctgggcc ggcggccaga
    20161 acgccgccgg cgtggcgcgc gcgcgccgct ggtggggcga gcaggtgaca tcgatccacg
    20221 acggctcctc caccagccca ccgctcaccg gcctgatgtg gtcggccatc gacgccgccg
    20281 cgccgcaggc cgaatacacc ggcatcgcgc tcgaattcgg caccgagccg atcctcgaga
    20341 ttctcgacgc cctgcgcggc gaccactggc tggcccagca ccccgaggcg ccgccggcgc
    20401 tggcggccga tatcaaggcg cgcctgcgtc gtgccttcta caccgacacc gacgcctgga
    20461 agggccagat cgtcagccag gcgcgccagg cgctgatgca ggcggtgcag gggctgagcg
    20521 aaggcggccg cggcacggcg gcctgaggcc ttgccctgcc accgcgccgg atcatccggt
    20581 ggcccccgat gccgcatcgg gcggggcagc gggcgtggtt tcgcgcgtcg gcggacacag
    20641 gcccagttca cgcagcaggc ccaggctttc ctgcgccgcc tgctcgcccg cctcgatggc
    20701 ctcgtgcgca cgatggaaat ccagcagcga caggtgcgac aggcgcggcg cgatggtgaa
    20761 ttccggcggg tcaccactca tgcggctgcg cgtgatgcgc acctgcatga tgtggatgcc
    20821 gctggtcagc acctccagca ccggcgggat cggcggtgtc gcatcctggg cggcggcctc
    20881 gtcgcggtgc cagcccagac tgcgctgcag gttctgcagc cagccgatcg acggctcgct
    20941 ggccggcacg gcggcctggg ccgggccgtg gcgcaggtgg cgcccgatca ggtcggcgtt
    21001 cagatcgacc gcgatcacga tgtcggcgcc cagcgcgcgc gccagtgaca ccggcaccgg
    21061 attgaccagc ccgccatcga ccagcaggcg cccctggtgc cacaccggcg tgaacaggcc
    21121 cggcatggcg atcgaggcgc gcacggcatc caccaccgag ccctggcgca gccagacttc
    21181 ctcgccggtg tgcagcgcgg tggcgacggc ggcaaacggc gtctccaggg catcgaagtc
    21241 acggtcggtg aactggctgc ggaaaaatcg catcagcttc tcgcccttga gcaggccacc
    21301 gccggtgctg atgtccatga acgacaccac atcgcgccgg cgcaggccca gcacccagcg
    21361 ctcgaagcgc tcggtcagcc ccagcacatg agccgcgccc accagcgccc cgatcgaggc
    21421 gccgcacacc acgccgggac ggatgccgca gtccgtcagc gcacgcagca caccgaaatg
    21481 tgcccagccg cgcgccgaac cgctgcccag cgccagaccg atgacgggcg cgcgtcgggc
    21541 aaaggcggcg tgagcgggtg gcgggtgggt catgcgtgcc gggctgcgtg ggacagggac
    21601 agtgtcgcac gggcggggcc gtgcggctgc cgctcaggtg ccgccggtgg cggtgcgccg
    21661 tgccatctcg gccagatggc gcgccgtggc gtcgcgcgcc atggccaccg aacccagcac
    21721 ctggccgtgg tcgtccctga ccacgccaaa gctcatgtcg acgtacagct tgcggccgtc
    21781 gcggtgcgtg ccgcgcgtgg tacgcacctc ggcgccgccg cgggtggcgc cgcgcgccat
    21841 ggcgcgccgg tacgccaccc agtgcggctc gcgcagccgt tccggaatga tgatgtccag
    21901 gctggcgccc agcgcttcgg cggcgctgaa gccaaacagc gcctcggcac ccgcattcca
    21961 ggcgcggatc acgccttcgg catcggcata gatcagcgca tcgggcacgt cgcgcaggat
    22021 gtggcgccag ggcggttcgg ggcaggcggt cggttcggtc atggcggcaa cggcgcaggc
    22081 ggacgaagaa ggcaggcccg catcctgccc ccaggcggcg cccgtggcaa gggcaggggc
    22141 ttgcggccgg tggcacccgc ctgcgcgcgc gctgccggac aatcgcgcca tcgcccaccc
    22201 gcggccacct caccatgtcc acgcccacgc cgacccgcat gccgacgctg tacatcccgc
    22261 acggcgccgg accctgcttc ttcatggact ggaacccgcc cgacacctgg gcgcgcaccg
    22321 ccgccttcct gcgcggcatt ccggacagcc tgccggccgc gccgaccgcc atcctgctgg
    22381 tgacggcgca ctggctggcg ccccagccca gcctgggcag cgccgcgcag cccggcctgc
    22441 tgttcgacta ccacggcttt ccgccccaca cctacgaact gagctacccc gcccctggtg
    22501 caccggcgct ggcccggcgc gccgccgccc tgctgaccga tgccggcttc gcgccgcggc
    22561 tggacgatgc gcgcggctat gaccatggca gcttcattcc gctcaagctc gcctttccgg
    22621 ccgccgacgt gccggtgctg cagatgtcgc tgctgcgcag cctggacgcg ggcgagcacc
    22681 tgcggctggg tcaggcgctg gcgccgctgc gcgacgaggg cgtgctgatc gtcggcagcg
    22741 gcatgagcta ccaccacatg cgcggctatg gcgatccgcg ctcgaccccg gtggcggccg
    22801 agttcgatcg ctggctggct gagacggtgg ccctgccggc ggccgagcgc cacgcccgac
    22861 tgaccgactg ggcgcgcgcc agcggccccg ccgggcgtct gagccacccg gcgcaggccg
    22921 aagagcacct gctgccgctg cacgtggcag ctggcgcggc cggcgacagc cgcggccgcc
    22981 gggtgttcac cgaccgcgtg atggcggtgg acatctcggc gttccgtttc gatgattcct
    23041 gaacccagaa acggcagttg accgcttgtc aacttcgggc gggccgcatc gatgcggggt
    23101 ctggcggctg cgatggcctt cacctcctgg gtgagcacgc tgcgcaccca cccgcaccgc
    23161 gctcggcgcg ccatgcggcg ttgctcctgc ctgcgggcag gagcccgcgg cgtcgtcgcg
    23221 tcttgcctgg cacgccgatc acggcacggg cgggcgcgtc caacggccgt ttctaggagg
    23281 ctgaaaccgc gtccgcctca gcgccccagc accagccccg ccgccagcgc cagggcgtgc
    23341 aggttggcgg cggcaatggt caggccgatg gccggccgca gccgcgccgc ctgccggcga
    23401 tggcgccaca ggagcccgcc ggcgaccagt gacagcggca gcgacagcag gccggccagc
    23461 gcgcccgtcg gggcccaccc ggccagtacc gccagcggca ccaccgcgtg cgccagcagc
    23521 gccagcccca ggtacaggcg cgccgccgcg gccggcccca ggcgcaccac cagggtgcgc
    23581 ttgccgaccc gcgcatcggc gggcgcgtcg ggaaacccgt tgatcagcag caggttggcc
    23641 accagcagcg ccagcccgcc acccagcagc gccggcaggg cggccagggc gccgcgctgc
    23701 acgtagtccg cccccagcac caccagccac cagcaggcgg ccaccgcggc ttcgcccagc
    23761 ccgcgcgcca tcagcgccag gggcggggcc gaataggccc aggccagcac cagcccggcg
    23821 gcgccgatca gccacaggcc agggccgctg acgctggcca gcaccacccc gccggcgctc
    23881 gtggccagca gcagccagag tgcccagcgc gcggtgtcgc gctcggtcac ctggccgtcc
    23941 tgaatcaggc gcgcaccgcc ggtgaagggc gacagcgcgt cccggttggc ggcgtcggca
    24001 ccgctgcggg cgtcgtgata gtcgttcaac acgttgcccc cggcgtgcgc cagacaggcc
    24061 agcagcagcg tcgccagcgc gcgccagagt gccagcggtc cggcgctggc ggcgctggcc
    24121 agcccgagca tgacggtcga caacgtcagc agcagaaagg ccggtcgcgt catgcgccag
    24181 cgcagccacg cctggtggcg ccagcgcgca gcggccggcg cctgcgaacc cccacccgat
    24241 ggcgcctggg gcgcgccccg ggtaagatgc gatgaatcgt tcataagcta ctattttaat
    24301 agctgctggc gcttgaacat caagcgccgc aagccgattt gatgcccgaa tctgacgccc
    24361 tgcaaagcca cgacgaggcg cgcatgcgac tgcgccggcg cgtcgccacg ctgctgtggc
    24421 gcgcgctggc gctgctgagc ctgctgctgg gtggcgtcgg ggccatgctg ccggtgttgc
    24481 cgacggtgcc gtttctgctg ctggccgcct gggccgccag caagggctgg ccggcgctgg
    24541 agatctggct gctcaaccac cgccggctcg gcccgccgct ggtgcagtgg cgcgaacgcg
    24601 gggcggtgtc gcgcccatcc aagtggctga gttcgatcat gatgggtggc agcgccatcg
    24661 gcatgcagtt tctgccgcag attccgctgg cgctgcgcct cggcgcgccg gcggtgatgc
    24721 tggcggtggc catctggctg tggcgacggc ccgaggcctg agcgccgccc gcctacactg
    24781 gcggccatgc acccgatcca gctctttgac ccgaccagct gcacctacac ctacctgctg
    24841 gccgaccccg ccacgcgcca ggcggtgatc atcgacccgg tcgacggcca gctcgagcgc
    24901 gacctggcgc tgctggagcg cgagggcctg acgctgaagt ggacgctgga aacccacgcc
    24961 catgccgacc acatcaccag cgccgcgcgc ctggtggaac tgaccggcgc gcacagcggc
    25021 gctccggccg cctgcgaggt cggcaccgcc gccgtgcagc tgcgcgacgg cgacacgctg
    25081 gacttcggcc gcgagcggct gcgcgtgctg cacacgcccg gccacacggc aggcagcgtc
    25141 tgctatctgt ggcagccgcg ccaggcgggc gcaccggcgc agctgttcac cggcgacacg
    25201 ctgctgatcg acggctgcgg gcgcaccgac tttcagtcgg gcgacgccgg ccagctgtac
    25261 gacagcatca cgcaaaagct gttcacgctg gccgacgaca cccgcgtctg gccggggcac
    25321 gactacgaag gccgcacgca ttccagcgtg gggcacgagc gcgctcacaa cgcgcgcctg
    25381 gccggccgca cgcgggccga gttcgtggcc ctgatgctgg cattgaacct gccgcacccg
    25441 cgccgcatcg acgaggcgct gcccgccaac cggcgctcgg gcctgcacca gatggcgggt
    25501 gcgccgctgg cgccggacga gcacggcgtg cgcgccgccg tgggctatgc cggcgatatg
    25561 tcgcccgcca cggcctgggc ctgggtgcag gccggccgcg ccgcactggt cgacgtgcgc
    25621 accgacgccg aacgcgagtg ggtcggcttt gtgcccggtg cgctggccgt ggcctggaag
    25681 cagtggcccg gcatggcgct caaccctgac tttgatgcgc agatgcgcgc cgccggccag
    25741 ggtggccggc cgctgctgct gatctgccgc agcggcgtgc gctccatcgc cgccgcgcgc
    25801 cgcgccaccg agctgggcct gacggcctac aacgtgaccg aaggttttga gggcgacccc
    25861 gaccagcacg cccagcgcgg ccacctgggc ggctggcggc atgcggggtt gccgtggcgg
    25921 cagaactgac ggtgcaaccg gccgggcgcc agcggctcag cgccgccggg cacgggctgc
    25981 gccgcccagc ccgaccagca gcgccagcgc gcccaggctc cattcggcca atgtgggcac
    26041 cggtgccacc gccgccgggg cggccgcctg agtgagggtg aagctttcgc tgttgtcgcc
    26101 gaagttggtg tcggcaacgg tgaaggtgat ggtcgggttg gcttgggaaa acgccggaaa
    26161 cccgagcgcc gccagcgtcg tggtcggcag cgtcagcccg gtgggcggcg agccggagcc
    26221 gagcccgttg gcggcattgg tcggccacac ctgtacggtg ccgacacccg aaatctgcat
    26281 cagcaacgcc ccgtaggtcc agctgccaaa accggtcgac gcccccacag gcgaagtacc
    26341 ggccaccacg atcacgccgg cgccattgac gcaataggtg ttgccggact gcaggcaggg
    26401 gttgccggtc tggctgaagg caatcgtgtc ggcctgcgtc agcgtgccgg tgtaggtgaa
    26461 tgacgcgccg gcggggctgg tgcccgggat caccagcgta ccccccggaa ggttgacgtt
    26521 ggcggcctgg gcagccgccg tgacgagggt ggcgcacacc agcgatgcga tccggcttgg
    26581 cttcatggga tggtctccgg taaaaccgac cattggccgg catcttgttt ggaaagcaga
    26641 caccccatcg actgcaatgc ccgcgcggtc atggtatcgg cgccaccgac gaacgccaat
    26701 gaatgtaact tttatttacg gtgtcgccac gggctgcacc atgcccgtca gaccggaggc
    26761 atcgtcgcca gccagtcgcc gatcagcgct tcatacgcgg cagccacgcg cagcagcgag
    26821 gcgtcaccct ggggctgacc gatcagctgc aggcccatgg gcaggccttt ggagccgaag
    26881 ccggcgggca cgctgatgca gggcaggccg gcgaagctgg cgtagatcac cacttccatc
    26941 cagcggtggt aggtgtccat ggtgcggccg gcgatctgct gcggccagcg ttcgcgggcg
    27001 tcgaacggcc agacctgggc gctgggcagg gccagcacgt cgtggcgcgc cagcagtgcg
    27061 cgcatgcgct gataaaaggc gctgcgggtg acgctggcgg ccatgaactc ggcggcgctg
    27121 cagccttgcg cctggtcgtg ctcccacagc gcctcgggct tgatgtgggc tcggttgccg
    27181 gttttcagca agaacggcgc gatgcgcgag gccaccagca cgcgccgcca gaccagccag
    27241 gcgtcccaca cacgctcggg cgacatgccc agccgcgccg gctcgaccac gctgccggcg
    27301 cgctcgaagc gcgccagcgc ctgcgtgcag gtgtccagca caccggcctc cagcggcaga
    27361 tatgcgtcca cgtcggcaag ccagccgatg cgcaggcctt tcaagttcaa cggcatgctg
    27421 gcgcttgcca gatctgcgcc ggcagctatc gaaagaggag catctggaga ccagccggcc
    27481 tgcgtggcca acagccgcgc cagatcgcgc acgctgcgcg ccatcggccc ttcggtgccc
    27541 agctggctga tccagacgtc ctgcgcgggc gccatgggca cgcggccctg cgaggggcgc
    27601 aggccgaaca ggttgttcca ggccgccggg ttgcgcaggc tgcccatgaa gtcggagccg
    27661 tcggccaccg gcagcaggcg cagcgccagc gccaccgccg cgccgccgct ggagccgccg
    27721 gccgacttgc tcgggtcgta ggcgttcagc gtggtgccga acaccgggtt gaaggtgtgc
    27781 gagccgaggc cgaactcggg cgtgttgctt ttgccgatga cgatgcagcc ggccgccttc
    27841 atgcgtgtgg tcatcaggcc gtcgtcgcgg gcaatgaagt ccttcatcag cggcgaaccc
    27901 agcgtggtgg gcaggccggc ggcgtgggcc aggtccttga tcgcctgcgg gatgccgtgc
    27961 agccagccgc gcgagtggcc gcgcgccagt tcggcgtcac aggcgtcggc ctcggccagc
    28021 agttcgtcgt cggggcgcag cgacaccagc gcgttgtggc gcgggttgag gcgctggatg
    28081 cgcgccagac aagccgccat cagctcgcgg caggacagct ggcgcgcatg gatggcggcc
    28141 gaaagcgcgc tggcgtccag ctcggtcggg tcggtgggca tggcggcggc gtgcatggcg
    28201 ccactctacg ccagcagcag ccatgaaaaa aggcatccga agatgccttt tcatgaacga
    28261 aatcaggctc cggcgcttgc agggcaagcg ccataagcta ttgatttcag agcggcggga
    28321 tgcgcagctt ctggcccggg tagatcttgt ccgggtggct cagcatcggc ttgttggctt
    28381 cgaagatcac cgggtacttg ttggcatcgc catagtattt cttggcgatg gcgctcagcg
    28441 tgtcaccgcg caccacgtcg tggtattgcg aagcggtgcc ggccgggtac tgcaggttgt
    28501 tgtcgacatc gctgacgctg gccacgttgc cagcggccag gccggccttt tcggccgctt
    28561 cctggctggg cgcattgcca cccagcgtga ccttgccgct ggcgccatca aagccgaccg
    28621 tcagacccga caggcccagg ttctgcgtct cgacgtaggt cttgatggcg tcggcagcct
    28681 tctggttcag ctcggccagg ttgggtgcgc ccgtggccac agcctgctcc acctccttgt
    28741 ggccaaacag cttttcgccg gcttccttga tgaagctgaa cagtcccata caggttctcc
    28801 ttggttgttg atgacaggaa cctttcactc taacgcagga cgatcgcgcg gcgccatgtc
    28861 ttcggtatcc aggcgtcaac gaatgcgctt atattgcgaa cccatgactt ttttggcgat
    28921 tgacgtcggc aacacccggc tgaaatggac gctgtatgag cgcccggcgg tcggtgcacg
    28981 ggtgctggcg cagggcgtgg agtttctgga gcagctcgat cggctggccg aaggaccctg
    29041 ggccgacctg cccgaaccca gccgcatgct gggctgcatc gtcgccggcc aggccgtcaa
    29101 gcaccgcgtg caggagcaga tggagcattg gggcgtggag ccacgctggg tggtggcgtc
    29161 ggaatcggaa gccgggttgg tcaacggcta cgacttcccc tcgcgcctgg gcgccgaccg
    29221 ctgggtggcc atggtcggcg cgcgccacca catgctgcag cgcggaccgg cccgcccggt
    29281 gctgctggtg atggtcggca ccgccgtgac ggtggaggcc atcgacgcgc aggggcgctt
    29341 tctgggcggg ttgatcattc ccgggcacgg catcatgctg cgtgcgctcg agtccggcac
    29401 ggccggcctg catgtgccca ccggcgacgt ggtggagttc cccaccaaca ccagcgatgc
    29461 gctgaccagc ggcggcacct tcgccatcgc cggcgcctgc gagcgcatgt tccagcacct
    29521 gttcaaacgc tgtgcggccg agccgctgtg cctgatgacg ggcggcgccg gctggaagat
    29581 gctgccggcc atgagccggc cctatgagtt gatcgagagc atgatcttcg acggtctgct
    29641 ggtcatcgcc gaggcacgcg aggccctgaa cggcgggttg tagcgagcgc tgcgggccct
    29701 gcccgccgcc tcgcatggcg cacctgacgc gcgcagcgac gcctaccgtc gggccactca
    29761 accggttccg cccaccgtca gaccgtcgat gcgcagcgtg ggctgaccga ccccgaccgg
    29821 cacgctttgg ccttccttgc cacaggtacc gacacccgag tccaggcgca tgtcgttgcc
    29881 gatcatgctg atcttcttga gcgattcggg cccgctgccg atgagcgtgg cgcccttgac
    29941 cggatattga atccggccgt tctcgaccca gtaggcctcg ctggccgaaa acacgaactt
    30001 gccgctggtg atgtcgacct ggccgccccc gaagttggtg gcgtacaggc ccttgcggat
    30061 gctggcgacg atttcgcgcg ggtcgcggtc gccgcccagc atgtaggtgt tggtcatgcg
    30121 gggcatcggg atgtgggcgt agctctcacg ccgcccgttg ccggtgggtt tgaccttcat
    30181 caggcgggca ttcagggtgt cctgcagata ggaccggagg atcccatctt cgatcaggac
    30241 attgcgctgc gtcacatggc cctcgtcgtc cacgttcagg ctgccgcggc gatcggccag
    30301 ggtgccgtca tcgagcaccg tcacgccttt ggcggcgacg cgctggccga tgcggccgct
    30361 gaaagcgctg gagcccttgc ggttgaagtc accctccagc ccgtggccga ccgcctcgtg
    30421 caacaacacg cccggccaac ccgggcccag gaccacggtc atttcgccgg ccggcgccgg
    30481 ccttgattcg aggttggtca aggcggcggc gaccgcctcg tccacgtact gcgccacccg
    30541 accggcatcg aagtgcgtca gcccaaagcg cccgccaccg ccggacgagc cgatctcgcg
    30601 ccgtcccccc tgctcggcca ccaccgtgac cgacagccgc accagcggac gcacatcggc
    30661 cgccagcgta ccatcaaggc gcgcgaccag caccacgtcg tattcgctcg ccagccctgc
    30721 catcacctgc accacgcgcg gatccctggc acgcgcgcgt tgctccacca tttcaagcaa
    30781 ggccaccttg gccccgctgt ccaacgtggc gatcggatcg atcggcgcat acagggggcg
    30841 attgcgcccg gacgcacgcc ggcgacgccg atcgacgcgc cccacccccg cagcagccga
    30901 aatggcacgc accgtgcatg ccgcatcctg cagcgaaccg ggagaaagat cgtccgaata
    30961 cgcgaacgcc gtgcgctcac ccgttaccgc ccgcaccccg acgccctggt cgatcgaaaa
    31021 actccctgtc ttgacgatcc cctcttccag gctccaaccc tcggtgcgcg tgtactgaaa
    31081 gaacaggtcg gcgtcgtcga cacggtgacg ggcgatggtc gccagcgtgg catccagctc
    31141 ccgctcggac agtccatacg ggtccagcaa caatgcgcgc gccgtggcca gatggtgatg
    31201 cttgatattg cgtgtgttca tcgagggcat tctaggctgt gctaccgaga agcgtctttc
    31261 actccgctcc gcaaggggcg atgcggccgc ccaaaagaaa acgcccagtc attgctgact
    31321 gggcgtttct tgctgtaata gcctgacgat gtcctacttt cacacgggaa tccgcactat
    31381 catcggcgct aaggtgtttc actgtcctgt tcgggatggg aaggagtggg accacctcgc
    31441 tatggtcatc aggcataact tgttgtcatt cagcaagata ctgaacaacc aatccataga
    31501 gcaaatcagc ttgatttgaa tgcgtcactt ggcataacaa ccttgatcga cgatcaaagt
    31561 tatagggtca agccg
//
LOCUS       DAOOOG010000011        30886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_1258_length_30886_cov_30.031916, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000011 DAOOOG010000000
VERSION     DAOOOG010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 30886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30886
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1258_length_30886_cov_30.031916"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="PK242_01965"
     CDS             complement(<1..1199)
                     /locus_tag="PK242_01965"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOZ92818.1"
                     /translation="MANPSRMANAIRALAMDAVQQAKSGHPGAPMGMADMAVALWTRH
                     LRHNPANPQWADRDRFVLSNGHASMLLYALLHLSGYELPLQELKDFRQLHSKTAGHPE
                     VGLTPGVETTTGPLGQGVTNAVGMALAEKLLAAEFNRPGHAIVDHHTYVFLGDGCLME
                     GVSHEACALAGAWKLNKLIALYDDNGISIDGQVAPWFVDDVPARFAAYRWNVIGPIDG
                     HDVDAVDRAIAQARRSSDRPTLIVCKTHIGKGSPNRQDSAKAHGEPLGTEEIALTRAA
                     LGWDAAPFEIPADVGAAWDARAAGQAREAEWTQRLAAYQAAHPELAAELLRRLRGELP
                     PNFHQVAVDAAVAAHQKAETVASRKASQIALEAFTAALPELLGGSADLTGSNLTNTSH
                     TPALRVDA"
     gene            1365..1814
                     /locus_tag="PK242_01970"
     CDS             1365..1814
                     /locus_tag="PK242_01970"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOZ92819.1"
                     /translation="MDPCTSPDIPAGFHPLQAGGGFMAFNGPLYLRHQGDDVRFGLRV
                     EPRHCNPMGNLHGGMMASFCDMLLPLSVHRKGAGLGQRFLPTISLQIDYLAPAPLGCW
                     IEGTAELLRATRTLVFAQGLVTADGEPCARASGVFKIGPEMPTELAG"
     gene            complement(1841..2332)
                     /locus_tag="PK242_01975"
     CDS             complement(1841..2332)
                     /locus_tag="PK242_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOZ92820.1"
                     /translation="MFDVTPLSATDRSEQYAELARQVDGLLHGETQLIANAANFAALV
                     FHALPELNWCGFYFAEGQELVVGPFQGKPACVRIAFGRGVCGTAAATRQTQVVPDVHA
                     FAGHIACDSASRSEIVVPLLRRDGSLLGVWDVDSPRPARFDEVDRVGMQALCARFLRA
                     CAL"
     gene            complement(2358..3707)
                     /locus_tag="PK242_01980"
     CDS             complement(2358..3707)
                     /locus_tag="PK242_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92821.1"
                     /translation="MKRLLITVALSFGAHLPAQAWSNHALASYRAFEPMPEVAQAPAV
                     LAEPLEQFLAAQAQPIADLLQAQDAWAQAHLPHYPPLPAALRFDPAVASQGPQALRRA
                     FLTALRVSPDSRLELYVQPDPWGPEPQAERLPHDAVSALPLRDKDGEKHRFVRLHAGQ
                     AVAPIAVLASASDEPDYGMDLNLWQDSPSSWGPGYGFGHIPFGNPSLDFATQAPFHMG
                     YYHESGLIYAAAGFLKRTYPLLRAHQYQGLAQLAFRRGHPYWGWRFAGLALHYVQDLT
                     QPYHASLAPGYSSARLIGIQLLALLGRPGAKNDMVVRLSNRHLVLERYESQMIQASAQ
                     ARKAGPLEQALRDTAADASYGPWSDTTLRDEVARQAHAAGETVTAQLLATVPAGYVDD
                     PTFDFGVHAGGIDLMAEVAQRGAPAKQALEQTLATLMRHFGVHSRRLVRAILQGAAP"
     gene            complement(3720..5282)
                     /locus_tag="PK242_01985"
     CDS             complement(3720..5282)
                     /locus_tag="PK242_01985"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOZ92822.1"
                     /translation="MTAPCLLAIDCGTQSVRALLFDLQGQLLARAQVAIDSYRAPEPG
                     WMEGAPEAFWQHLCAACHRLWATGTVPREAIAGVVLTTQRAVPVTLDADGQPTRPSMI
                     WLDQRRAARAPRLPWWWEAALTAVGMRAAVRHFQHEAEACWIAQHQPELWARTRHFLL
                     LSGYLNWRLCGRLADSVAAQVGYVPFDYRRGRWAAPWDWKWHALPVRRAMLPELLPPG
                     ARLGTVSAEAAQASGIPAGLPLIAGAADKACEVLGAGCLSPEVACLSYGTAATVNITT
                     ARYLEATRFIPPYPAAVPGHFNPEVQITRGFWMVRWFREQFGQHEERLARERGVAPET
                     LFDELVGTVPPGAQGLTLQPFWNPGIKQPGPEAKGAIIGFGDVHTRAHLYRAILEGLA
                     YALREAAERVERRRGIAIERVRVAGGGSQSDAALQITANVFNRPAERPHLYETSGLGA
                     AIIASVGLGLQPDFASAVRAMTRVGQVFAPQPEHAHTYERLYRRVYLRMYRQLAPLYR
                     DIRDITGYPSRD"
     gene            complement(5476..6393)
                     /locus_tag="PK242_01990"
     CDS             complement(5476..6393)
                     /locus_tag="PK242_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ92823.1"
                     /translation="MDLSSIDLNLLRILHRLLQERHVSRAAQDLGLSQPAVSNALRRL
                     RVQLGDDLLVRSAAGMQPTPLAQRLAGPIAQALELLDDALRAQAPFDPADSVRQFTLA
                     MSDVGELYFLPVLMRALAQLAPGVTLRTTALDSPGLGPSLSTGEIDLALGSLPRLQGG
                     FYQQSLFRQGYLCLMRNGHPMAEGPLTRAAFLACEHVRVEAPGTGHGRVDSEFERRGI
                     RRRIRLSVPNYVALGHVLASTDLIATVPERFAERVCAALALRTHPLPVRIASGVIRQF
                     WHQRSHRDSGHEWLRRQVSSLFADSATRA"
     gene            6514..7806
                     /locus_tag="PK242_01995"
     CDS             6514..7806
                     /locus_tag="PK242_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HOZ92824.1"
                     /translation="MPSTSLDWPAGDTSRVPFAIYTNEQIHRKELQRFFYRGHWCYVG
                     LEAEVPRPGDFKRTVVGERSVILSRAADGQLHCFENVCAHRGMQFCRKRHGSGMKEFV
                     CPYHQWSYTLSGELQGVPLRRGVRQDGRVLGGMPADFDTKAHGLTRLKVATRGGVVFA
                     SFDHDVEPLEDYLGPTILPYFDRLFNGRQLKILGYNRQRIPGNWKLMQENIKDPYHPG
                     LLHTWFVTFGLWRADNKAALRMDAHHRHAAMISTRGNAGKQSDVSQVSSFKAAMELED
                     KRFLDIVHEDWWGEPTVVMTTVFPSVIFQQQVNSVSTRHIQPDGHGHFDFVWTHFGFA
                     DDTAEMTARRLRQANLFGPAGFVSADDGEAIELSQCGFTQKPGHHALVEMGGRGVEET
                     DYMATETLIRGMYDYWRKVMERPDQASDAVAPQEGAQA"
     gene            7803..8279
                     /locus_tag="PK242_02000"
     CDS             7803..8279
                     /locus_tag="PK242_02000"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HOZ92825.1"
                     /translation="MRLDFDTWQALVQLQADYAAVVDGGDWDRWPRFFTEDGVYKLQP
                     RENHERGFPLCTLAFDCQAAMRDRVYGIKETLFHDPYYQRHVVGAPLVHAADSDGIRC
                     ESHYAVFRTKLDGLSSVFNVGRYLDRVVRTPLGLKLAERLVVYDSEMIPNSIIYPI"
     gene            8295..8606
                     /locus_tag="PK242_02005"
     CDS             8295..8606
                     /locus_tag="PK242_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HOZ92826.1"
                     /translation="MSQWIDVAAEDDIFEGAAIAVTPIGHDIALYKTEAGEVFATHNI
                     CSHGHARLCEGFLEGHEIECPFHQGRFDVRTGQATLAPCVDPIRSYPVKIEAGRVWVQ
                     L"
     gene            complement(8673..10466)
                     /locus_tag="PK242_02010"
     CDS             complement(8673..10466)
                     /locus_tag="PK242_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOZ92827.1"
                     /translation="MKLPQIRELATQLRFAPEEGRIWMDERRMVLVHSDTFADLREEL
                     LASLGYEMTRGLLTRIYFAAGQRDAASVLKRLGPGASLKEVLNTGGRMHALQGYLLPE
                     HVGRGLAAGDLRSDDYYGEALWKDSLEDEAHVARHGVGQHAVCWNAVGYCSGYLSYCA
                     QRTIVVREVECRASGGNHCKIVAQPAAQWDNVEEDLRFLLPQPVGRRARIVAPVADGG
                     PTTALAQSAVTDSRDDDRAAPRSLAETAFVGASTAFSVLRHKIDRVAPTQATVLLLGE
                     SGVGKSLIARQVHRRSQRAQAQLIEVNCAAIPEALVESELFGVERGAFSGASQARAGR
                     FEAAHGGTLFLDEVATLSPTTQGKLLRVLQSGALERLGSNQTRVTDVRVIAATNEDLK
                     QAVRQGRFREDLYFRLNVFPVVVPPLRERRDDIPLLVQALLQRFAPLHGRGHVGITSR
                     ALQALIHHDWPGNIRELENVIERAVIMLHEGEMLDLQHLSYADDTLNTPSYFGLDDTG
                     QLMINTDYDFGASQSPASTSAASPAALDMNRLADDLLRQGPGQLAAMERALVRSAVRQ
                     AGGNITRAAALLGLSRAQTEYRYRKQQSAPG"
     gene            10465..10731
                     /locus_tag="PK242_02015"
     CDS             10465..10731
                     /locus_tag="PK242_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92828.1"
                     /translation="MSTTTCGICRTGARGVLSGMAGRGCCAMIHKNLHEIMKSINFGT
                     WVASDMRGITRGERGKPTKAQRQTEGFPLDLGQSFLAGTVLAEL"
     gene            10775..11674
                     /locus_tag="PK242_02020"
     CDS             10775..11674
                     /locus_tag="PK242_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ92829.1"
                     /translation="MASISNLGYLVLGVKDLEAWKQFAVDTIGLQPGRSEPGKLLALR
                     MDGYEQRIVLERHDDDDMLAAGWELDTQEDLNAFAAQLRQQGVAVQQGDAALAKERRV
                     EDLYFCTDPNGIRHEFYVGAQRASMVNPFHSSKLVGGFSTGRLGMGHFVAAANDAQET
                     IAFCKQVLGIKISDFIRGELAPGVLLDVAFFHARTGRHHSMATVKIPFPYPKRIHHIM
                     VEVDNPDDVGLAYDRCVQAGYTINMGLGRHPNDRMFSFYVHTPSGFLLEFGAGGIVVD
                     DADWEIKSYTRLSEWGHHHPEPH"
     gene            11808..12668
                     /gene="bphD"
                     /locus_tag="PK242_02025"
     CDS             11808..12668
                     /gene="bphD"
                     /locus_tag="PK242_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
                     hydrolase"
                     /protein_id="HOZ92830.1"
                     /translation="MLIHTDEDTSRFITIDEPGLENFQLHYNDAGSGPAVIMLHGGGP
                     GASGWSNFYKNIDHFVDHGFRVLLLDCPGFHKSDELVSDQVRPLLNARAVKGLMDGLG
                     IERAHLLGNSLGGATALHFALEFPERLDRLVLMGPGGLGHSIMQANPQEGIRHMIKLY
                     KQPSYAHFESMLEVFVYNPAAITEELRQGRWANIQARPNHLSNFLRCVALSPIATWDM
                     MARAHQIQHPTLITWGRDDRFVPIDHGLRLLHTLGKAELHVFSQCGHWAQWEHAPAFN
                     ELVINFLKRA"
     gene            12688..14454
                     /locus_tag="PK242_02030"
     CDS             12688..14454
                     /locus_tag="PK242_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOZ92831.1"
                     /translation="MNTVVTDVLVIGTGPAGAATALCLARAGVKVLAVSKYHATSPDP
                     RAHITNQRTMEFLRDFGLEEAAKKIAMPWEDMGEHVFGRSLVGEEWGRIPAWASSAIA
                     QGEHMAASPSRYCDLPQLYLEPLLVNDAGLRGADIRFKTEYLSHTQDAEGVTATLLDR
                     VSGSEYQVRAKYLVGADGGRSKVAADIGLEFDGAMGLGETGNINIEIQADLSQFCAHR
                     RSDMYWMLQTGENLMGLPQGPGIAVLRMVRPMDRWVCVVGYALAKGLPSLKHEDLLPF
                     VHRTIGSDQVPVKILSVSTWTTNRQYALTNTQGRVFCIGDAVHRHTPFGGLGSNTCIQ
                     DAYNLSWKLAMVIQGQAGASLLNTYQDERAPIAKQITEHAFNGGASMGPLLMALGVPP
                     GSGPEAMEQAVARLKEPSAEGAERRAKLRQAMDGTLNGFGRAHGIELNQRYRSSAIVS
                     DGTPDPGFARDPVQYHQASSRPGAHLPHAWVTRGQRKISTFDLCGQGRFTLLTGLSGM
                     AWKATAEQAAAALGIELVVHVIGFGQEVVDSHGDYLRISEVEESGALLVRPDLMVAWR
                     AANADSTQLAQLPSVLRQILGR"
     gene            14607..15383
                     /locus_tag="PK242_02035"
     CDS             14607..15383
                     /locus_tag="PK242_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOZ92832.1"
                     /translation="MPHPAPRALILAAGRGERMRPLTDRCPKPLLPLRGKPLMQWALE
                     ALLRAGVRELVVNTAWLGQQIEDHFAAQRLSDVRQQLSISEHIGLHFSSEGRDFGGAL
                     ETAGGIVRALPLLAPGARDVFWVAAGDVFAPDFDFAADAVRRFVASDRLAHLWLVPNP
                     AHHPAGDFAIGADGLAQNDAPAGGRRHTFSTIALYRRALFEPPWCDIPAGNPQGVAAP
                     LAPLLRRAIAAGRVGAELYQGPWTDVGTPERLAALNAGVS"
     gene            15392..16780
                     /locus_tag="PK242_02040"
     CDS             15392..16780
                     /locus_tag="PK242_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOZ92833.1"
                     /translation="MSHPTPYAARRARVAAQLGPDAIALIPTAPARPRNRDSDHPYRH
                     DSYFYYLTGFTEPDAWLVLTGDGRSTLFCPPKDLEREIWDGIRLGPEAAPAALGVDEA
                     YSVHELNARLPGLLENRRSLWFPFAIHEGLSARVDGWLQQVRARVRYGALCPGEQHDL
                     CRVLDELRLIKDAGELATMRRAGQISAAAHVRAMQRSAAMLRAGQDVREYHLEAELLH
                     EFRQHGAQSPAYPSIVAAGANACVLHYRAGDAAIRAGELVLIDAGCELDGYASDITRT
                     FPADGRFAGPQRALYELVLASQQAAIAATRPGARFTDPHEAAVRVLTQGMLDLGLIDK
                     GQHGTLEDAIAHRAYFAHYMHRTGHWLGMDVHDCGSYVEPGEVGQPSPRRDPLSGELI
                     QDRPSRILRPGMVLTIEPGLYVRPSEKVPSAFWNIGIRIEDNAVVTADGCELLSRGVP
                     VEPDAIEALMRA"
     gene            complement(16850..17485)
                     /locus_tag="PK242_02045"
     CDS             complement(16850..17485)
                     /locus_tag="PK242_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92834.1"
                     /translation="MNRWQRSAWPLACLLLIVASALAGLAVASGRLPPELLSWRRGDW
                     PHAPWTLWSGALLHRLAPHALANMLALAALAVLGAALGARPRDALALLVAWPLGTLGL
                     RCWPQVAAYWGLSGPIHAAAAVLALRALTAADTRWLGLLLGGGLSVKLALEHGWESPI
                     AFDAGWGFNVVFAAHLSGAIAGALLAVALNVAGWLRLASASATDQRSSHGK"
     gene            complement(17686..20511)
                     /gene="glnE"
                     /locus_tag="PK242_02050"
     CDS             complement(17686..20511)
                     /gene="glnE"
                     /locus_tag="PK242_02050"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HOZ92835.1"
                     /translation="MTPADVQGSHGDGESLLSDFRGLPGSAAHSRLVQRLRRRYADEL
                     PLLPAGTPTRASMQDCLTALAARGHALGPSLRILRQLVMERLVVLDCEQGAPLHDITG
                     AVTELAELALDAAFTDAFAQLDERHGEPLTEAGQRPRFWVIGMGKLGARELNVSSDID
                     LIYLYEEDGQTRGRADGRGQISVHEYFGRAARLAQTLIGDTTEHGFVFRVDLALRPNG
                     ESGPIVCSLDALEEYMLAQGREWERLAWLKSRALAPRADLDGDAARALRAVVLPFVFR
                     KYLDYNVFESLRTLHRQIREHASKRSAGRPERANDVKLSRGGIREIEFTVQLLQVVRG
                     GQFPELRTRPTLAALERVAHAGLMPPDTADALARAYVFLRRVEHRIQYLDDQQTHVLP
                     TQDEDLDWIARTLGYPSACPFLADLCAHRERVAHEFDGLLGGDAPCKSCGKKSADAPA
                     PAPHDLPAVLDAFPERVRERLAPWQDHPRVRALREEALGRLLRLLQRTAAWLDEGRVS
                     DDAVLRLADWVEPLLRRESYLALLLERPAVHERLLRVLGTARWPARYVMQHPGVIDEL
                     AGPGLLSGRFDALAFEQELERRRQALASTGEDDDESLLNLLRRAHHAETFRTLVRDVE
                     RRITVEQVADDLSALADAILRLTTRWCWPRLRQRHREQPRFAIIAYGKLGGLELGYGS
                     DLDLVFVFDDEHEDAPDIYAALARKLINWLTAQTREGDLYEIDTALRPNGNSGLLVTS
                     FDAYADYQQNRGSNTAWTWEHQAMTRARLLAPRLAADAAAPGGAAAEPAFDLSARFQA
                     VRHAVLTAPRDHAALRAEIIAMRERLQAAHPVRAGLFDLKHSPGGMIEAEFATQYLVL
                     AHTREHPELEPNLGNIALLIRAEQVGLLPAGVGQGAADAYRHLRHLQHLARLDEQSTQ
                     IDPATVTEQRAAIQRLWQAVFTPASRA"
     gene            20631..24701
                     /locus_tag="PK242_02055"
     CDS             20631..24701
                     /locus_tag="PK242_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HOZ92836.1"
                     /translation="MRWVVRGVGVLLALVLLAAAILHWVIVPRVDEWRPRLERLASQA
                     IAAPVQIGAIEAQSNGLVPSVALRDVRVLDPDGRPALQVPRVLAAFSVLSLGTLGLEQ
                     LVIDAPELELRRTRDGRLLAGGIDLSGDATGDTRAADWFFSQDEIIVRDGRVRWVDEQ
                     RAAPPVQLSAVLFVSRNGYGRHQLRLDATPEPAWGERFTLIGQFRQRVFSRHPGYWRE
                     WDGQAFASAPRIDVSRLRQYVDLTADWGVDVQAGHGALRLWLEQKQGRPVAATADLAL
                     GAVSVTLGPRLAPLAFSELTGRLAWRDPGDGMTLGTRDLRFVDADGLTWPGGNLRLAL
                     HDAAGRHPGGGEFSGDRLDLAALAKIAQRLPLPPAVHERLQAHPVQGLVERIEARWQG
                     PLEAPTDWRVQTRVSGLALAAPAQATPATGEPVAGIPGIEGAALELEATPAGGRASLA
                     IADGALTFPGVFEEPRIPLARLDLRGRWRVQGEQLAVEVEQLDLRNADATGSFKARWQ
                     TLAGAQGAARFPGLLDLSGSFSRANGARVHRYLPLVIPAEARHYVRDAIVRGDARDVA
                     VRVRGDLRQLPFDQHPEAGEFRFAGPVQGVTMAYVPRALQPAGQPPWPALEGLAGELI
                     FERNSMRVRQASARVQGHPGWRFTRVQADIDDLQHARVRVEAEGRGVLASALDIVRGS
                     PVAGFIEHALDRASASGDASLQLGLDLPIDAIDRSRVNGRVALAGNELRIAPEAPLLT
                     QAQGVVTFSETGFAVADARVQLLGGQARLSGGSTAAAQGGPTVLLRASGSASAEGLRE
                     MADWAPLPALARQATGSASYEAQIGFRGGEPEVRVTSDLRGLAFDLPAPLDKPADAAW
                     PLRYDSGPVAGSAGRQRLRVEVAQRLAIEYEHAADSDRVLRGLIGLGPRATALALPAS
                     GVQAGIELPHLDVERWRDALQRVLGGGATLSAEGGRLPSQWTLRTAELVVDGRSLNDV
                     VASGTQQGGQWRGEVQARELAGRIDFREGADGRAGKLSARLARLSIPAGGSDDRTLLA
                     QPPAQIPALDVVVEQFELRGKALGRLEIQAVNHDVAGPAAAGETLQNWELSRLSLQAP
                     EAHLTAHGRWAALARGPGLPLDPRGPRAPEDRRRTQLEFKLDIRDAGKLLARLDMPDV
                     LARGRGELRGGLGWRGTPFSPHYPSMAGQLHLDVDAGQFLKADPGVAKLLGVLSLQAL
                     PRRLTLDFRDLFSTGFAFDFIRGDVEVQAGIARTNNLQMKGPSAAVLMDGEADLDHET
                     QRLRVLVVPEIDAGTAALVATAINPAIGIGAFVAQLVLKRPLIKAATREFQISGRWDD
                     PQVTPLGAPAADAQTPAPATPPTPRSATDTPEEERS"
     gene            24698..25528
                     /locus_tag="PK242_02060"
     CDS             24698..25528
                     /locus_tag="PK242_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOZ92837.1"
                     /translation="MKVAAIQMVSGSRVEDNLHRAQALLAQAAQAGAELAVLPEYFCL
                     MGQRDTDKLALAEPLGDGPIQRWLADSARALGLWLVGGTLPLAPDGAAPPLTHCRNAT
                     LVFSPAGERVAVYDKIHLFQFDDGTRRYDEAAVLRPGRQPVRFDLPSRDGHRWRVGLS
                     ICYDLRFPELYRQHAADGAELLLVPSAFTHVTGQAHWEVLLRARAIENLCGVVAAAQG
                     GTHDNGRRTWGHSMVVDPWGQVLAVQDEGAAVVLAELEPAQLAACRARLPALRHRVLG
                     "
     gene            25525..27579
                     /locus_tag="PK242_02065"
     CDS             25525..27579
                     /locus_tag="PK242_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOZ92838.1"
                     /translation="MSATAPVRAAASPDPGSAAALRRLRAGAGRRWLLWGVLSLTVVG
                     LLAIAVWLARGHEIEQVQRALERDSLEAVQVLRERLGRNLQDLHGLGAAPADPEAWSA
                     GAARLLETHREWLRLEWRGAALEPRAARDSPFHTPLPAEVREAMAPELQRLCQQASVQ
                     GEAIYAPPSFVLRAGGLGREVMTLCLPLQDGARTQGFVLASYTLDELLRLLPTSLTRG
                     QAVAFTELDGTRLALHGAARRGARLFVAQQTLDLPGAPMLLRLEGGRPLPDVFPNVLT
                     ALVTLLSMALLTALGLLARDIRRRQQAEAELAEALAFRKAMEDSLITGLRARDLQGRI
                     TYVNPAFCAMVGYTPQELIGQSTPAPYWPPERAHEYGARQALRLSGQAAAPREGVETE
                     FMRKDGTHFPVLIFETPLITAQGQHSGWMGAVVDMTAQRRAEESSRASLERLQASARL
                     ATVGEMASLMSHELNQPLAAIASYASGSLNLLPAGAGGDAATARELRTALERIAEQAG
                     RAGRVIHSVRELVRQRSTAPQAVAPRALVDAVLPLVQLQARKLGASVRVQVAPELPAV
                     WCEPTMIEQVLLNLARNGLQAMAEVPGERLLTLQARLPTAPAGPALLEFGVADTGTGM
                     DAAVVERLFTPFFTTKPEGMGLGLSLCRTVIEQHGGTLVHAPNLPRGTLFRFTLAVAD
                     AP"
     gene            complement(27629..28591)
                     /locus_tag="PK242_02070"
     CDS             complement(27629..28591)
                     /locus_tag="PK242_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ92839.1"
                     /translation="MQVNPTPARSRAVLGQVSDMDLRLLQVFKAVVECGGLSAAELEL
                     NIGTSTVSRHVKDLETRLGLTLCRRGRGGFAVTPEGQRVYDETRRLLAAVDGFRASVD
                     DIHQRMGGTLAVALFDKTVSNPQARIHAAIADFTQQAPDVALHLHVGSIQTIERGVMD
                     GSFAVGVIPAHRASASLRYQRLFGETMHLYCARPHALFDSEHSALDWPALRARQDFAG
                     LGYHSPNMELAHGQRLRRGATAYDQEAIATLILSGRFIGFLPDHYAAAFVQRGQLRAV
                     APERLHYDCDFVSVLRRSPQPARAALAFHRALLAAHADAMTHDA"
     gene            28703..30058
                     /locus_tag="PK242_02075"
     CDS             28703..30058
                     /locus_tag="PK242_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOZ92840.1"
                     /translation="MSVTATVPAPAATRRDAAWLEAHWMPFTGNRQFKRDPRLIVAAQ
                     GAYFTDADGRQVFDGLSGLWCSGLGHGRREIAEAVGRQAAQLDYAPAFQFGHPLSFEL
                     ANAIKQRTPQGLDHVFFTASGSEAADTALKMARAYWRARGQGGKTRLIGREKGYHGVN
                     FGGISVGGMVGNRKLFGQGIEADHLPHTQPPAGTFAKGQPAEGGVALADRLLELIALH
                     DASNIAAVIVEPFSGSAGVVIPPVGYLQRLREICTQHDILLIFDEVITGFGRCGAMTG
                     SDAFGVVPDLMTFAKQVTNGVQPLGGVVASRAIYDTFMAAGGPDYMLEFAHGYTYSAH
                     PVACAAGLAALELLEREDGPGRVKALSAHFEAAVHSLRGARHVLDIRNYGLAAGFTIA
                     PVAGEPARRPYDIAMACWKKGFYVRYGGDTIQLAPPFISEKAEIERLVNALGDALAEV
                     A"
     gene            complement(30381..>30886)
                     /locus_tag="PK242_02080"
     CDS             complement(30381..>30886)
                     /locus_tag="PK242_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ92841.1"
                     /translation="GLDLPVYPVKGYSLTVPLLDPANAPRSTVMDETYKVAITRFDQR
                     IRVGGMAELGGFDLRLNPRRRETLELVLGNLFTGGDIPRAEFWTGLRPMTPDSTPVVG
                     ATRYANLLLNTGHGTLGWTMAAGSGKLVADLTLGRQSDISTAGLGLERYGRSQAGPRS
                     LPRHVPA"
BASE COUNT         4485 a        10813 c        11051 g         4537 t
ORIGIN      
        1 gcgtcgacgc gcagcgccgg cgtgtggctg gtgttggtga ggttggagcc ggtcaggtcg
       61 gcgctgccac ccagcagctc gggcagcgcg gcggtgaagg cctccagcgc gatctggctg
      121 gccttgcggc tggccacggt ctcggctttc tggtgcgccg ccacggcggc gtccaccgcc
      181 acctgatgga agttcggcgg cagctcgccg cgcaggcggc gcagcagctc ggccgccagc
      241 tccgggtgcg cggcctggta ggccgccagg cgctgcgtcc actcggcttc gcgggcttgg
      301 ccggcggcgc gcgcatccca ggcggcgccg acgtcggccg ggatctcgaa cggtgcggcg
      361 tcccagccca gcgcggcgcg ggtgagggcg atttcctcgg tccccagcgg ctcgccatgc
      421 gccttggcgc tgtcctggcg gttcgggctg cccttgccga tgtgggtctt gcagacgatc
      481 agcgtcggcc ggtcgctgct gcggcgcgcc tgcgcgatgg cgcgatcgac ggcgtcgacg
      541 tcgtggccgt cgatcggccc gatcacattc caccgatagg cggcaaagcg cgccggcacg
      601 tcatcgacga accagggtgc gacctgaccg tcgatgctga tgccgttgtc gtcgtacagc
      661 gcaatcagct tgttcagttt ccaggccccg gccagcgcgc aggcctcgtg gctgacgccc
      721 tccatcaggc agccgtcgcc caggaacacg taggtgtggt gatcgacgat ggcgtggccg
      781 gggcggttga actcggccgc cagcagcttc tcggccagcg ccatgccgac cgcgttggtc
      841 acgccctggc ccagcgggcc ggtggtggtt tccacgccgg gggtcagacc gacctcgggg
      901 tggccggcgg tcttgctgtg caactggcgg aagtccttca gttcctgcag cggcagctcg
      961 tagcccgaca gatgcagcag ggcgtacagc agcatcgacg catggccgtt ggacagcacg
     1021 aagcggtcgc ggtcggccca ctgcgggttg gccgggttgt gcctgaggtg ccgcgtccac
     1081 aacgccacgg ccatgtcggc catgcccatg ggggcgcccg ggtggcccga cttggcttgt
     1141 tgtacggcgt ccatggccag ggcgcggatc gcgttggcca tgcgggaagg atttgccatg
     1201 gagcggctcc ggaaggcggt gcaggagggg ggaaaccggc gattttaccg ggccccccag
     1261 ggccggcgcg gcgacgccgc gcatggcccc gcaagccggg cggctgtcgc ctgcggcacg
     1321 cgcgccgcac cgccgccacg tggcgcgggc ttatcctgcg ccccatggac ccttgcactt
     1381 caccggacat cccggccggc tttcatccgc tgcaggcggg cggcggcttc atggccttca
     1441 acggcccgct ctatctgcgg caccagggcg acgacgtgcg cttcggcctg cgggtggagc
     1501 cgcgccactg caatcccatg ggcaacctgc acggcggcat gatggccagc ttctgcgaca
     1561 tgctgctgcc gctgtcggtg caccgcaagg gcgccgggct cggccagcgt tttctgccca
     1621 ccatcagcct gcagatcgac tacctggcgc cggccccgct gggctgctgg atcgagggca
     1681 cggccgagct gctgcgcgcc acccgcacgc tggtgttcgc gcagggcctg gtgaccgccg
     1741 atggcgaacc ctgcgcgcgc gccagcgggg tgttcaagat cgggcccgag atgccgaccg
     1801 agctggccgg ctgagccgcc gcgctggggc tggcggagcg tcagagcgcg caggcgcgca
     1861 gaaagcgcgc gcacagcgcc tgcatgccga cgcggtcgac ctcgtcgaag cgcgccggcc
     1921 gcgggctgtc cacgtcccac acgcccagca ggctgccgtc acggcgcagc agcggcacca
     1981 cgatttccga gcgcgaggcg ctgtcgcagg cgatgtggcc ggcaaaggca tgcacgtccg
     2041 gcaccacctg ggtctggcgc gtggccgccg ccgtgccgca caccccgcga ccgaaggcga
     2101 tgcgcacgca ggccggtttg ccctgaaacg gcccgaccac cagctcctgg ccttcggcaa
     2161 agtagaagcc gcaccagttc agctcgggca gggcgtgaaa caccagcgcg gcaaagttgg
     2221 cggcgttggc gatcagctgc gtttcgccgt gcagcagccc gtcgacctgg cgcgccagct
     2281 cggcgtattg ctcggatcgg tcggtggcgg acaggggggt gacgtcgaac atgggtcagg
     2341 gtggatcggt gcttgtgtca gggtgcggcg ccttgcagga tggcgcgcac cagccgccgg
     2401 ctgtggacgc cgaaatggcg catcagcgtg gccagggtct gttccagcgc ctgcttggcg
     2461 ggcgcgccgc gctgcgccac ctcggccatc aggtcgatgc cgccggcgtg cacgccgaag
     2521 tcgaacgtcg ggtcatcgac gtagccggcg ggcacggtgg ccagcagctg cgccgtcacc
     2581 gtttcgccgg cggcgtgcgc ctggcgcgcc acttcgtcgc gcagcgtggt atcgctccac
     2641 gggccgtagc tggcgtcggc cgcggtgtcg cgcagcgcct gctccagcgg gccggccttg
     2701 cgggcctggg cgctggcctg gatcatctgg ctttcgtaac gctccagcac caggtggcgg
     2761 ttggacaggc gcaccaccat gtcgttcttg gcgccgggcc gtcccagcag ggccagcagc
     2821 tggatgccga tcaggcgcgc gctcgagtag cccggcgcca ggctggcgtg gtagggctgg
     2881 gtcaggtcct gcacgtagtg cagcgccagc ccggcaaagc gccagcccca gtacgggtgg
     2941 ccgcggcgaa aggccagctg cgccagtccc tggtactggt gggcgcgcag cagcgggtag
     3001 gtgcgtttca gaaacccggc cgccgcgtag atcaggcccg actcgtggta gtagcccatg
     3061 tgaaacggcg cctgggtggc gaagtccagg ctggggttgc cgaacgggat gtggccgaag
     3121 ccgtagcccg ggccccagct ggaggggctg tcctgccaca ggttcaggtc catgccgtag
     3181 tcgggttcat cgctggccga cgccagcacg gcgatcgggg ccacggcctg gccggcgtgc
     3241 aggcgcacga agcggtgctt ttcgccgtcc ttgtcgcgca gcggcagcgc gctgacggcg
     3301 tcgtgcggca ggcgctcggc ctgcggctcg gggccccagg ggtcgggctg cacgtacaac
     3361 tccagccggc tgtcgggcga cacgcgcagc gccgtcagga acgcccgccg cagcgcctgc
     3421 ggaccctggc tggccaccgc cgggtcaaag cgcagggccg ccggcagcgg cgggtagtgc
     3481 ggcagatgcg cctgcgccca ggcgtcctgc gcctgcagca ggtcggcgat cggctgggcc
     3541 tgcgcggcca ggaactgctc cagcggctcg gccagcacgg ccggggcctg ggccacctcg
     3601 ggcatgggct cgaaggcgcg gtagctggcc agcgcgtggt tgctccaggc ctgggcggga
     3661 agatgggcgc cgaagctcag cgcgacggtg atcagcagac gtttcatggg cagggcggtt
     3721 cagtcgcgtg aagggtaacc cgtgatgtcg cggatgtcgc ggtacagcgg cgccagctgg
     3781 cggtacatgc gcaggtacac gcgccggtac aagcgctcgt aagtgtgcgc atgctcgggc
     3841 tggggcgcaa acacctggcc cacgcgcgtc atggcgcgca ccgcgctggc gaagtcgggc
     3901 tgcagcccca gccccacgct ggcgatgatg gccgcgccca ggccgctggt ctcgtacagg
     3961 tgcgggcgtt cggccggccg gttgaacacg ttggcggtga tctgcagcgc ggcgtcgctc
     4021 tgcgagccgc cgccggccac gcgcacccgt tcgatggcga tgccgcggcg ccgctcgacg
     4081 cgctcggccg cctcgcgcag cgcgtaggcc agtccttcca ggatggcgcg gtacaggtgg
     4141 gcgcgcgtgt gcacgtcgcc aaagccgatg atggcgccct tggcttcggg gccgggttgc
     4201 ttgatgcccg ggttccagaa cggctgcagc gtcaggccct gggcgcccgg cggcacggtg
     4261 cccaccagtt cgtcgaacag ggtctcgggc gccaccccgc gctcgcgcgc caggcgctct
     4321 tcgtgctgac cgaactgctc ccggaaccag cgcaccatcc agaagccgcg cgtgatctgc
     4381 acctcggggt tgaagtggcc cggcacggcg gccgggtagg gcgggatgaa gcgcgtggct
     4441 tccaggtagc gtgcggtggt gatgttgacc gtggccgccg tgccgtagga caggcaggcc
     4501 acctcgggcg acaggcagcc cgcgcccagc acctcgcagg ccttgtcggc ggcgccggcg
     4561 atcagcggca ggccggccgg aatgccgctg gcctgcgccg cctcggcgct cacggtgccc
     4621 agccgcgcgc cgggcggcag cagctcgggc agcatggcgc ggcgcaccgg cagcgcgtgc
     4681 catttccagt cccagggcgc ggcccagcgc ccgcgccggt agtcgaacgg cacatagccc
     4741 acctgcgccg ccaccgagtc ggccagccgc ccgcacaggc gccagttcag gtagcccgac
     4801 agcagcagaa agtgcctggt gcgcgcccac agctcgggct ggtgctgggc gatccagcag
     4861 gcctcggcct cgtgctggaa gtggcgcacc gccgcgcgca tgccaaccgc cgtcagcgcc
     4921 gcctcccacc accagggcag gcgcggcgcc cgggcggcgc ggcgctggtc gagccagatc
     4981 atcgacgggc gcgtcggctg gccgtcggcg tccagcgtca ccggcaccgc gcgctgggtg
     5041 gtcagcacca cgccggcgat cgcctcgcgc ggcaccgtgc cggtggccca cagccgatgg
     5101 caggcggcgc acaggtgctg ccagaaggcc tcgggcgcgc cttccatcca gcctggctcg
     5161 ggcgcgcggt agctgtcgat ggccacctgc gccctggcca gcagctggcc ctgcaggtcg
     5221 aacagcagcg cgcgcacgct ctgcgtgccg cagtcgatgg ccagcaggca gggcgcggtc
     5281 atggggtggt ggggtggcgg cgcaggcgat gtagtcgcac cgcctcgcct gcaggcgcgt
     5341 gtgccatcgt aacccgagca agcgcggcag ccggggcacg cggcagccgg gtgcgggtgg
     5401 tggtgaacat gttcgccgcc ggatgaatct gatctgccag cgccgtcgcg acgccgacag
     5461 gtcgggcgag agtcatcagg ctcgggtggc gctgtcggcg aacaggctcg atacctgccg
     5521 ccgaagccat tcgtggccgc tgtcgcgatg tgaacgctgg tgccagaact ggcgaatgac
     5581 accggaggca atgcgcacgg gcaggggatg cgtgcgcagc gccagcgcgg cgcagacccg
     5641 ttcggcgaag cgctccggca ctgtggcaat caggtcggtg ctggccagca cgtggcccaa
     5701 cgccacgtaa ttcggcaccg acagccgaat ccgccgcctg atgcctcggc gctcgaactc
     5761 gctgtcaacg cggccgtgac cggtgcccgg agcctccacg cgcacgtgct cgcaggccag
     5821 gaacgcagcg cgcgtcagcg ggccctcagc catcgggtgc ccattgcgca tcaggcaaag
     5881 atagccttgg cgaaacagcg attgctggta gaagcctcct tgcaggcgag gcagcgaacc
     5941 cagcgccagg tctatctcgc cggtggacag cgatggcccc agcccggggc tgtccagcgc
     6001 tgtggtgcgc aaggtgactc cgggcgccag ctgggccaga gctcgcatga gcacgggcaa
     6061 gaaatagagc tctcccacat cggacatggc cagcgtgaac tggcgcacgc tgtcggctgg
     6121 gtcgaatggc gcctgcgcac gcagcgcatc gtcgagcagt tccagcgctt gtgcgatggg
     6181 gccagccaag cgttgggcca ggggcgtggg ctgcatgcca gccgcgctgc gcaccagcag
     6241 atcgtccccc agctgcacgc gcagtctgcg cagcgcattg ctcaccgccg gctgcgacag
     6301 acctaggtct tgcgcggcgc gtgaaacgtg gcgctcctgt agcaggcgat gcaggatacg
     6361 cagcaggttg aggtcaatgg aggacaaatc catctcgaca ttattctcaa acgaaatatc
     6421 acacattcca gatgcgagct tgaggcaggg actcatgctc ggcatcatgt caggcaaggc
     6481 gcgtgcctgc atcacttacg gagacttggc ttgatcccgt ccacatcgct tgattggccc
     6541 gctggcgaca ccagccgcgt gccattcgcc atctacacca acgagcaaat ccaccgcaag
     6601 gagttgcagc gcttcttcta ccgtggccac tggtgctacg tcggtctgga ggctgaagtg
     6661 ccccgtcctg gcgatttcaa gcgcaccgtg gtgggtgagc gctcagtgat cctcagtcgt
     6721 gccgcagatg gccagctgca ttgtttcgag aacgtgtgcg cacaccgcgg catgcagttc
     6781 tgtcgcaagc ggcatggcag cggcatgaag gagttcgtct gcccctacca ccagtggagc
     6841 tatacgctca gtggcgaact gcagggggtg ccgctgcgtc gtggcgtgcg ccaggatggg
     6901 cgggtgttgg gcggcatgcc cgcggatttc gacaccaagg cgcatggttt gacacggctc
     6961 aaagtcgcca cccggggtgg tgtcgttttc gccagcttcg accatgatgt ggaaccgctg
     7021 gaggattacc tgggccctac gatcctgccc tacttcgatc gattgttcaa cggacgtcaa
     7081 ttgaagatcc ttggctacaa ccgccagcgc atcccaggca actggaagct gatgcaggaa
     7141 aacatcaagg acccctatca cccgggtttg ctgcatacct ggttcgtcac tttcggtctt
     7201 tggcgcgccg acaacaaggc agcgctcagg atggatgcgc accatcgcca cgcggccatg
     7261 atctcgacgc gcggcaatgc cggcaagcag tccgatgtgt cgcaagtgtc gagcttcaag
     7321 gccgccatgg aactggagga caagcgcttc ctggatatcg tgcacgagga ctggtggggt
     7381 gagccgacgg tggtcatgac caccgtgttc cccagcgtga tcttccagca gcaggtcaac
     7441 agcgtgtcga cgcggcacat ccagccggac ggccatggcc atttcgactt cgtctggacg
     7501 cacttcggct ttgccgacga tacggccgag atgacggcgc gccgtctgcg ccaggccaac
     7561 ctgttcggtc cggccggttt cgtgtcggcc gacgacggcg aagcgatcga gctgtcgcaa
     7621 tgcggcttca cccagaagcc ggggcaccac gcgctggtcg agatgggcgg gcgcggcgtc
     7681 gaggagaccg actacatggc caccgagacg ctgatccgtg gcatgtacga ctactggcgc
     7741 aaggtcatgg agcgccctga ccaggcgtcc gatgcggtcg cgccgcaaga gggggcgcag
     7801 gcatgaggct cgatttcgac acctggcagg cgctggtgca gttgcaggcc gactatgccg
     7861 ccgtcgtcga tggtggcgac tgggaccgct ggccccggtt tttcaccgag gacggtgtgt
     7921 acaagctgca accacgcgaa aaccatgagc gcggctttcc gctgtgcacc ctggcctttg
     7981 actgccaggc ggccatgcgc gatcgcgtct acggcatcaa ggagacgctg ttccatgacc
     8041 cgtactacca gcgccacgtg gttggcgcgc cgctggtgca cgccgcagac agcgacggca
     8101 tccgctgcga aagccactac gcggtgttcc gcaccaagct cgacggactg tccagcgtgt
     8161 tcaacgtggg ccgctacctg gaccgcgtgg tgcgcacgcc gctcgggttg aagctggccg
     8221 agcgccttgt ggtgtacgac agcgagatga ttccgaactc catcatctac ccgatctgag
     8281 caggaaatcc gaacatgagc caatggatcg acgtcgccgc cgaggacgac atcttcgagg
     8341 gcgcggccat cgccgtcacg ccgatagggc acgacatcgc gctctacaag accgaggcgg
     8401 gcgaagtctt cgccactcac aacatctgct cgcatggcca tgcgcggctg tgcgagggtt
     8461 tcctcgaagg tcacgagatc gagtgcccgt tccaccaggg gcggttcgac gtgcgcaccg
     8521 gccaagccac tctggcccca tgtgtcgacc cgatccgcag ctatcctgtc aagattgagg
     8581 ctgggcgcgt ctgggtccag ctctgacccc agcctaagac gcgggcggcc agggtcggag
     8641 gcctcgcctc ctttgcccgc gtctacgcga cgtcatcccg gcgcgctttg ctgcttcctg
     8701 taccggtatt cggtttgtgc ccgcgacaga cccagcagtg cggcggcacg agtgatgttg
     8761 ccccccgcct gccgcacggc gctgcgcacc agggcgcgct ccatggcggc cagctgaccg
     8821 gggccctggc gcagcaagtc atcggccaac cggttcatgt ccagtgccgc aggactggcc
     8881 gcactcgtcg atgccggtga ctggcttgcg ccgaagtcgt aatcggtgtt gatcattaac
     8941 tgccccgtgt cgtccaggcc gaagtagctc ggggtgttca gggtgtcatc ggcgtaggac
     9001 aggtgctgca agtccagcat ttcgccttca tgcagcatga tgacggcgcg ttcgatgacg
     9061 ttttccagct ctcggatatt gcccggccag tcgtggtgga tcagtgcttg cagtgcccgc
     9121 gacgtgatcc caacgtggcc acgcccgtgt agcggggcaa agcgctgcag cagcgcctgc
     9181 accagcagtg gaatgtcgtc gcgccgctcg cgcagtggcg gcaccaccac cggaaagaca
     9241 ttgaggcgaa agtaaaggtc ttctcgaaag cgtccctgtc ggacggcctg cttaaggtct
     9301 tcgttggtgg cggcaatcac gcgtacgtcg gtcacgcggg tctgattgct gcccagacgc
     9361 tccagcgcac cgctttgcag cacgcgcagc aacttgccct gggtggtcgg gctgagcgtt
     9421 gcgacctcgt ccagaaacag cgtgcctcca tgcgcggcct caaagcgccc ggcgcgggcc
     9481 tgactggcgc ccgagaaggc cccgcgctcg accccaaaca gctccgactc caccagcgcc
     9541 tctgggatgg cggcgcagtt gacttcaatc agttgcgcct gggcgcgctg gctgcgtcgg
     9601 tgcacctggc gtgcgatcag gctttttccc accccgcttt cgcccagcag cagcaccgtg
     9661 gcctgggtgg gggcgacgcg gtcgatcttg tgccgcagca cggaaaaggc ggtcgaggcg
     9721 ccaacaaagg cggtttctgc cagcgaccgc ggcgccgcgc ggtcatcgtc tctgctgtcg
     9781 gtgacggcgc tctgggcaag ggcagtggtg ggcccgccat cggcgacggg ggcgacgatt
     9841 ctggcgcgcc ggcccacggg ctgcggcagc agaaaacgca gatcctcctc cacgttgtcc
     9901 cattgggcgg caggctgagc gacgatcttg cagtggttgc caccgctggc gcggcactcg
     9961 acctcgcgca ccacaatggt gcgttgcgcg cagtagctca ggtagcctga gcagtagccc
    10021 acggcattcc agcagacggc gtgctgaccc acgccatggc gggcaacatg tgcctcgtct
    10081 tcgagcgagt ccttccacaa ggcctcgccg taatagtcgt cgctgcgcaa gtcgccggcg
    10141 gccaacccac ggccgacatg ctcaggcagc agatagcctt gtagggcgtg catgcgtcca
    10201 ccggtgttga gcacctcttt cagactcgcg cccggaccca gcctcttcag cacgctggcg
    10261 gcatcgcgct ggccggcagc gaagtagatg cgcgtgagca gcccgcgcgt catttcatag
    10321 cccaggctgg ccagcagctc ctcgcgcagg tcggcaaagg tgtcgctgtg caccaggacc
    10381 atacgccgct catccatcca gatgcgccct tcctcgggcg caaatcgcaa ctgcgtcgcc
    10441 agctcgcgga tttgcggcag cttcatgtcc accaccactt gcggcatttg caggacgggg
    10501 gcgcggggtg tgctatcggg catggcgggc cgcggctgct gcgcaatgat tcacaagaat
    10561 cttcatgaaa ttatgaaatc catcaatttt ggaacctggg tcgcctcaga tatgcgaggg
    10621 ataacccggg gcgagcgagg aaaacccaca aaagcgcagc ggcaaacaga gggttttccc
    10681 ctggatttgg gtcaatcctt cctggccggc acggttctcg cagagctctg atatccctga
    10741 ccgcgtgggc ggggtcgttt ataggagaca gacgatggca agcatttcga acctggggta
    10801 tctggtgctg ggtgtgaagg acctggaagc ctggaagcaa ttcgccgtgg acaccatagg
    10861 cctgcaaccc gggcgcagcg agcccggcaa actgctggcg ctgcgcatgg atggctacga
    10921 acagcgcatc gtgctggagc gtcatgacga cgatgacatg ctggccgccg gctgggagct
    10981 cgatacccag gaagatttga atgcctttgc cgcccagctg cggcagcagg gtgtggccgt
    11041 gcaacagggc gacgcggcgc tggccaaaga gcggcgcgtg gaagacctgt acttctgcac
    11101 cgaccccaac ggcatccggc acgagttcta cgtcggcgcg caacgcgcca gcatggtcaa
    11161 cccctttcat tccagcaagt tggtgggcgg tttttccact ggccgcctgg gcatggggca
    11221 tttcgtggca gcggccaacg atgcgcagga aaccatcgcc ttttgcaagc aggtgctggg
    11281 catcaagatc agtgacttca ttcgtggtga gctggccccg ggcgtgctgc tggacgtggc
    11341 gtttttccat gctcgcaccg ggcggcacca ctccatggcc acggtgaaga tccccttccc
    11401 ctatcccaag cgcatccacc acatcatggt cgaggtggac aaccccgacg acgtcggtct
    11461 ggcctacgac cgctgcgtgc aggcgggtta caccatcaac atggggttgg gtcgccatcc
    11521 caatgaccgc atgttctcgt tctacgtgca cacaccttca gggttcttgc tggagtttgg
    11581 tgccggcggc atcgtggttg acgacgccga ctgggagatc aagagctaca cccgcctgag
    11641 cgaatggggc caccaccacc ccgagccgca ctgaaaggcc aggcgccatg caatggcttg
    11701 atgtcgagaa gaacagccgc gccaccgcga ccattctgcg ccagcagcca tcacgactga
    11761 atgagtacca gcggtgcgca gcactgcgaa tgactgttga gatcaccatg cttatccata
    11821 ccgacgaaga caccagccga ttcatcacca tcgacgaacc cggcctggaa aacttccaac
    11881 tgcactacaa cgacgccggc agcggccccg cggtcatcat gctgcacggt ggtggcccag
    11941 gcgccagcgg ctggagcaac ttctacaaga acatcgacca cttcgtggac catggctttc
    12001 gcgtgctgct gctggattgc ccgggcttcc acaagagcga cgagctggtg tccgaccagg
    12061 tgcgcccgct gctcaatgcc cgtgccgtca agggtctgat ggacggcctg ggcatcgagc
    12121 gggcgcacct gctgggcaac tcactgggcg gggccacggc gctgcacttc gcgctggaat
    12181 tccccgagcg cctggaccgt ctggtgctga tggggcccgg cggcctgggc cacagcatca
    12241 tgcaggccaa cccgcaagag ggcatacgcc acatgatcaa gctctacaag caacccagct
    12301 atgcccactt cgaaagcatg ctggaggtgt ttgtctacaa ccccgccgcc atcaccgaag
    12361 agctgcgaca gggtcgctgg gccaacatcc aggcgcgacc caatcacctg agcaacttcc
    12421 tccggtgtgt ggcgctgtcg cccattgcca cctgggacat gatggcccgg gcgcaccaga
    12481 ttcaacaccc cacgctgatc acctgggggc gcgacgatcg cttcgtgccc atcgaccacg
    12541 gcctgcgcct attgcacacc ctgggcaagg ccgagctgca tgtgttttcg caatgtggcc
    12601 actgggctca gtgggagcat gcgccagcgt tcaatgagct ggtgatcaat ttcttgaaac
    12661 gcgcttgaca cacaagggga tgctgctatg aacaccgtag tgaccgacgt actcgtcatc
    12721 ggcactggcc ccgccggtgc cgccaccgcc ctgtgcctgg cgcgcgcggg cgtgaaggtg
    12781 ctggccgtca gcaaatacca cgccacctca cccgacccgc gcgcccacat caccaaccag
    12841 cgcaccatgg agttcctgcg cgacttcggt ctcgaagagg cggccaagaa aatcgccatg
    12901 ccctgggagg acatgggcga acatgtgttt ggccggtcgc tggtgggcga ggagtggggg
    12961 cgtattccgg cctgggccag ctcggccatc gcccagggcg agcacatggc cgccagcccc
    13021 agccgctatt gcgacctgcc ccagctgtac ctggagccgc tgctggtcaa cgacgccggt
    13081 ctgcgcggtg ccgacatccg tttcaagacc gaatacctgt cgcacactca ggacgccgag
    13141 ggcgtgaccg ccaccttgct ggaccgtgtc agcggcagcg agtaccaggt gcgcgccaaa
    13201 tacctggtgg gcgccgacgg cggccgctcc aaggtggcgg ccgatatcgg gctggagttc
    13261 gacggagcca tgggcctggg cgagaccggc aacatcaaca tcgaaatcca ggccgatttg
    13321 tcgcagttct gcgcccaccg ccgcagcgac atgtactgga tgctgcaaac cggcgagaac
    13381 ctgatgggtc tgccccaggg ccccggcatc gcggtgctgc gcatggtgcg ccccatggac
    13441 cgctgggtct gcgtggtggg ctatgcgctg gccaagggcc tgccctcgct caagcacgaa
    13501 gacctgttgc ccttcgtgca ccgcaccatc ggcagcgacc aggtgccggt aaagatcctg
    13561 tcggtctcca cctggaccac caaccgtcaa tacgcgctga ccaataccca gggacgggtg
    13621 ttctgcatcg gcgacgccgt gcaccgccac accccgtttg gcggtctggg ctccaacacc
    13681 tgcattcagg atgcctacaa cctgagctgg aagctggcca tggtcatcca aggccaggcc
    13741 ggcgccagcc tgctgaacac ctaccaggac gagcgcgcgc cgattgccaa gcaaatcacc
    13801 gagcacgcct tcaacggcgg tgccagcatg ggcccgctgc tgatggcgct gggcgtgccc
    13861 ccgggcagcg gccccgaggc catggagcag gctgtggcgc ggctgaagga gcccagcgcc
    13921 gaaggggccg agcgccgcgc caagctgcgc caggccatgg acggcaccct gaacggcttt
    13981 ggccgtgccc acggcataga gctcaatcag cgctaccgct ccagcgccat cgtcagcgac
    14041 ggcacgcccg accctggctt tgcacgcgac ccggtgcagt accaccaagc cagcagccgc
    14101 cccggcgcgc acctgccgca cgcctgggtg acgcgcgggc agcgcaagat ctccaccttc
    14161 gacctgtgcg gccaagggcg cttcaccctg ctgacgggcc tgtctggcat ggcctggaag
    14221 gccaccgccg aacaggccgc agccgcccta ggcatcgagc tggtggtgca cgtcattggc
    14281 tttggccagg aggtggtgga cagccacggc gactatttgc gcatcagcga agtcgaggaa
    14341 agcggcgcac tgctggtgcg ccccgacctc atggttgcct ggcgcgcggc caatgccgat
    14401 tccacccagc tggctcagct gccgagcgtg ctgcggcaga ttcttgggcg ctgacccgcc
    14461 gctccctttc cggctgcgtg cgacctttca ggccgggtgg ttgaccggca tcggtgcctg
    14521 cgcctgcagg gtgtaaggtg aacccttgcc gaaggtgttc gaccttgggg gcgggaggcc
    14581 gtctgctgat ccactacagt cgcccgatgc cccatcccgc cccccgcgcc ctgatcctgg
    14641 ccgccggccg cggcgagcgc atgcgtccgc tcaccgaccg ctgtcccaaa ccgctgctgc
    14701 cgctgcgcgg caagccgctg atgcagtggg cgctggaggc gctgctgcgc gcgggcgtgc
    14761 gggagctggt ggtcaatacc gcctggctgg gtcagcagat tgaagatcat tttgcggccc
    14821 agcgcttgtc cgacgtgcgc caacagctat cgatttcaga gcacatcggg ttgcatttct
    14881 cgtccgaagg gcgcgatttc ggcggcgcgc tggagaccgc ggggggcatc gtgcgcgccc
    14941 tgccgctgct ggcgccgggc gctcgcgacg tgttctgggt cgctgccggc gacgtgttcg
    15001 cgcccgactt cgactttgcc gccgacgcgg tgcggcgctt cgtcgccagc gaccgcctgg
    15061 cccacctgtg gctggtgccc aacccggcgc accatccggc gggcgacttc gccatcggcg
    15121 ccgacgggct ggcgcagaac gacgccccgg cgggcgggcg gcgccacacc ttcagcacca
    15181 tcgcgctgta ccggcgcgcg ctgttcgagc cgccctggtg cgacatcccg gccggcaacc
    15241 cgcagggcgt ggcggcgccg ctggcgccgc tgttgcgccg cgccatcgcc gccggtcggg
    15301 tgggcgccga gctgtaccaa ggcccctgga ccgacgtcgg cacgcccgag cggctggcgg
    15361 cgctgaacgc cggcgtgtca tgatcgacgc catgagccac cccacgcctt acgccgcccg
    15421 ccgcgcccgc gtcgccgccc agctgggccc cgacgccatc gccctcattc ccaccgcgcc
    15481 cgcgcgcccg cgcaaccgcg acagcgacca cccgtaccgc cacgacagct acttctatta
    15541 cctgacgggg ttcaccgagc cggacgcctg gctggtgctg accggcgacg gccgcagcac
    15601 gctgttttgt ccacccaagg acctggagcg cgaaatctgg gacggcatcc gtctgggccc
    15661 cgaggcggcg ccggccgcgc tgggcgtgga cgaggcctat tcggtccacg agctgaacgc
    15721 gcgcctgccc ggcctgctgg aaaaccgccg cagcctgtgg tttccgttcg ccatccatga
    15781 ggggctgtcg gcgcgcgtcg acggctggct gcagcaggtg cgcgcgcgcg tgcgctacgg
    15841 ggcgctgtgc ccgggcgagc agcacgacct gtgccgcgtg ctggacgagc tgcgcctgat
    15901 caaggacgcc ggcgagctgg ccaccatgcg ccgcgccggc cagatcagcg ccgccgccca
    15961 cgtgcgcgcc atgcagcgca gcgccgccat gctgcgcgcc ggccaggacg tgcgcgaata
    16021 ccacctggaa gccgagctgc tgcacgaatt ccgccagcac ggcgcgcagt cgccggccta
    16081 ccccagcatc gtggccgccg gcgccaacgc ctgtgtgctg cactaccgcg ccggcgacgc
    16141 cgccatccgc gccggcgagc tggtgctgat cgacgccggc tgcgagctcg acggctacgc
    16201 cagcgacatc acgcgcacct ttccggccga cggccgcttt gccggcccgc agcgcgcgct
    16261 gtacgaactg gtgctggcca gccagcaggc tgccatcgcc gccacccggc cgggcgcgcg
    16321 cttcaccgac ccgcacgagg cggcggtgcg ggtgctgacg cagggcatgc tggatctggg
    16381 cctgatcgac aagggccagc acggcacgct ggaagacgcc atcgcccacc gcgcctactt
    16441 tgcgcactac atgcaccgca ccggccactg gctgggcatg gacgtgcacg actgcggcag
    16501 ctacgtggag ccgggcgagg tcggccaacc gagcccgcgg cgcgacccgc tgtcgggcga
    16561 gctgatccag gaccgcccca gccgcatcct gcgccccggc atggtgctga ccatcgagcc
    16621 cggcctgtac gtgcgcccga gcgagaaggt gcccagcgcg ttctggaaca tcggcatccg
    16681 catcgaggac aacgccgtcg tcaccgccga cggctgcgag ctgctgtcgc gcggcgtgcc
    16741 ggtcgagccg gacgccatcg aggcgttgat gcgggcctga tgcgggggcg cgttccgatc
    16801 accaatgacg aatgaccttg ctgcgcttcg atgaccgaag ggtcacgcgt catttgccat
    16861 ggcttgagcg ctgatccgtg gcgctggcac tcgccagtcg cagccagccc gccacgttca
    16921 gcgccacggc cagcagcgcc cccgcgatgg cgccgctcag gtgggcggcg aacaccacgt
    16981 tgaagcccca gccggcgtcg aaggcgatgg gcgactccca gccgtgctcc agcgccagct
    17041 tgacgctcag cccgccgccc agcagcagac cgagccagcg cgtgtccgcg gccgtcagcg
    17101 cgcgcagcgc cagcactgcg gcggcggcgt ggatcgggcc cgacaggccc cagtaggcgg
    17161 ccacctgcgg ccagcagcgc aggcccagcg tgcccagcgg ccaggccacc agcagcgcca
    17221 gcgcgtcgcg cggccgcgcc cccagcgccg cgcccagcac cgccagcgcc gccagcgcca
    17281 gcatgttggc cagggcgtgc ggcgccagcc ggtgcagcag ggcgccgctc cacagcgtcc
    17341 agggcgcgtg cggccagtcg ccacgccgcc agctcagcag ctcgggcggc agccggccgc
    17401 tggccaccgc cagcccggcc agcgcgctgg cgacgatcag cagcaggcag gccagcggcc
    17461 aggcgctgcg ctgccagcgg ttcatggggc gcccacccgg cccatcggcg gccgctgcat
    17521 gctatgaata aatgagctgc tggcgcgcac cagtcgggcg ccagaggcca aaaccattcg
    17581 gaaatcggtg gggtggtgcg gccgcggcac gcggacgccc gagcggtggt cctgagcgaa
    17641 gccgcgacct gccatgccca tgggtggtgc gtgccgtgcg ccttgtcacg cccggctggc
    17701 gggcgtgaac accgcctgcc acagccgctg gatggcggcg cgctgctcgg tcacggtggc
    17761 cgggtcgatc tgggtgctct gttcgtccag ccgcgccagg tgctgcaggt ggcgcaggtg
    17821 gcggtaggcg tcggcggcgc cctggccgac gccggcgggc agcagcccga cctgctcggc
    17881 gcggatcagc agcgcgatgt tgcccaggtt gggctccagt tccggatgct cgcgcgtgtg
    17941 ggccagcacc aggtactggg tggcgaactc ggcctcgatc atgccgccgg ggctgtgctt
    18001 gaggtcgaac aggccggcgc gcaccgggtg cgccgcctgc aggcgctcgc gcatggcgat
    18061 gatttcggcg cgcagcgcgg cgtggtcgcg cggcgcggtc agcacggcgt ggcgcacggc
    18121 ttgaaagcgt gcgctcaggt cgaacgccgg ctcggccgcg gcgccgccag gcgcggcggc
    18181 gtccgccgcc aggcgcggcg ccagcaggcg ggcgcgcgtc atggcctggt gttcccaggt
    18241 ccaggcggtg ttgctgccgc ggttttgctg gtagtcggcg taggcgtcga agctggtcac
    18301 cagcaggccc gagttgccgt tcgggcgcag cgcggtgtcg atctcgtaca ggtcgccctc
    18361 gcgcgtctgc gcggtcagcc agttgatcag cttgcgcgcc agcgcggcat agatgtcggg
    18421 cgcgtcctcg tgctcgtcgt cgaacacgaa caccaggtcc aggtcgctgc cgtagcccag
    18481 ctccagcccg cccagcttgc cgtaggcgat gatggcaaag cgcggctgct cgcggtggcg
    18541 ctggcgcagg cgcggccagc accagcgggt ggtcaggcgc aggatcgcgt cggccagcgc
    18601 cgacaggtcg tcggccacct gctcgacggt gatgcggcgc tcgacgtcgc gcaccagggt
    18661 gcgaaaggtc tcggcgtggt gggcgcggcg cagcaggttc agcaggcttt cgtcgtcgtc
    18721 ttcgccggtg ctggccaggg cctgacggcg gcgctccagt tcctgctcga acgccagcgc
    18781 gtcaaagcgc cccgacagca ggccggggcc ggccagctcg tcgatcacgc cggggtgctg
    18841 catgacgtag cgcgccggcc agcgcgccgt gcccagcacg cgcagcaggc gttcgtgcac
    18901 cgccgggcgc tccagcagca gggccaggta gctttcgcgc cgcagcagcg gctcgaccca
    18961 gtcggccagg cgcagcacgg cgtcgtcgct gacgcggccc tcgtccagcc aggcggcggt
    19021 gcgctgcagc aggcgcagca ggcggcccag ggcctcctcg cgcagcgcgc gcacgcgcgg
    19081 gtgatcctgc cacggggcca ggcgttcgcg cacgcgctcg ggaaaggcgt cgagcaccgc
    19141 gggcagatcg tgcggcgccg gcgcgggggc gtcggcgctt ttcttgccgc aggacttgca
    19201 gggggcgtcg ccgcccagca gaccgtcgaa ctcgtgcgcc acgcgctcgc ggtgggcgca
    19261 cagatcggcc agaaaggggc aggcgctggg gtagcccagg gtgcgggcga tccagtccag
    19321 gtcttcgtcc tgggtgggca gcacatgggt ctgctggtcg tccaggtact ggatgcggtg
    19381 ctcgacgcgg cgcaggaaca cgtaggcgcg cgccagcgcg tcggcggtgt cgggcggcat
    19441 caggccggcg tgcgccacgc gctccagcgc cgccagggtg gggcgggtgc gcagttcggg
    19501 gaactggccg ccgcgcacca cctgcagcaa ctgcaccgtg aattcgattt cgcggatgcc
    19561 gccgcgcgag agcttgacgt cgttggcgcg ctcgggccgg ccggcgctgc gcttgctggc
    19621 gtgctcgcgg atctggcggt gcagcgtgcg cagcgactcg aacacgttgt agtcgaggta
    19681 cttgcggaac acgaagggca gcaccacggc gcgcagcgcc cgcgcggcgt cgccgtcgag
    19741 gtcggcgcgc ggcgccagcg cacggctctt gagccaggcc aggcgttccc actcgcggcc
    19801 ctgcgccagc atgtattcct ccagcgcgtc gagcgagcag acaatggggc cggactcgcc
    19861 attggggcgc agcgccagat cgacgcggaa cacgaacccg tgctcggtgg tgtcgccgat
    19921 cagcgtctgg gccaggcggg cggcgcggcc aaagtattcg tgcaccgaga tctggccgcg
    19981 accgtcggcg cggccgcggg tctggccgtc ttcctcgtac aggtagatca ggtcgatgtc
    20041 gctggagacg ttgagctcgc gcgcgcccag cttgcccatg ccgatgaccc agaagcgcgg
    20101 gcgctggccg gcctcggtca gcggctcgcc gtggcgctcg tcgagctggg cgaaggcgtc
    20161 ggtgaaagcg gcgtccagcg ccagctcggc cagctcggtg acggcgccgg tgatgtcgtg
    20221 caggggcgca ccctgttcgc agtccagcac caccaggcgc tccatcacca gctggcgcag
    20281 gatgcgcagg ctcgggccca gcgcgtggcc gcgtgcggcc agcgccgtca ggcagtcttg
    20341 catcgacgcc cgcgtggggg tgcccgcagg cagcagcggc agctcgtcgg cgtagcggcg
    20401 gcgcagccgc tgcaccaggc gcgaatgggc ggcgctgccc ggcagaccgc gaaagtcgct
    20461 taacaaagat tcaccgtcgc cgtgagaacc ttgcacatcg gcaggggtca taatttctgt
    20521 cgtttctccg aggcgccagc cggcgcgcgc cgcgcctgcg ctgggccttg ctcctgtctt
    20581 cgttagcaag ccgcttggtc caatcgttct tccaccttgc cgccgtcctg atgcgctggg
    20641 tcgtccgcgg tgtgggcgtg ttgctggcgc ttgtgctgct ggccgcggcg atcctgcact
    20701 gggtcattgt gccgcgtgtc gacgaatggc gcccgcggct ggagcggctg gcctcgcagg
    20761 ccatcgcggc gccggtgcag atcggcgcca tcgaagcgca gtcgaacggc ctggtgccct
    20821 cggtggcgct gcgcgacgtg cgcgtgctcg acccggatgg ccgcccggcg ctgcaggtgc
    20881 cgcgcgtgct ggcggcgttc tcggtgctgt cgctgggcac cctggggctg gagcagctgg
    20941 tgatcgacgc ccccgagctg gagctgcgcc gcacccgtga cggccggctg ctggccggtg
    21001 gcatcgacct gtcgggtgac gccaccggcg acacccgcgc ggccgactgg ttcttctcgc
    21061 aggacgaaat catcgtgcgc gatggccgcg tgcgctgggt ggacgagcag cgcgccgcgc
    21121 cgccggtgca actgagcgcg gtgctgttcg tttctcgcaa cggctacggc cgccaccagt
    21181 tgcggctgga cgccacgccc gagccggcct ggggcgagcg cttcaccctg atcggtcaat
    21241 tccgccagcg cgtgttctcg cgccacccgg gctactggcg cgagtgggac ggccaggcct
    21301 tcgccagcgc gccgcgcatc gacgtttcgc ggctgcgcca gtacgtcgat ctgacggccg
    21361 actggggcgt cgacgtgcag gccggccacg gcgcgctgcg cctgtggctg gagcagaagc
    21421 agggccgccc ggtggccgcc acggccgatc tggcgctggg cgcggtgtcg gtcacgctgg
    21481 ggccgcgcct ggcgccgctg gccttcagcg aactcaccgg gcgcctggcc tggcgcgatc
    21541 ccggcgacgg catgacgctg ggcacgcgcg atctgcgctt cgtcgacgcc gatggcctga
    21601 cctggccggg cggcaacctg cggctggcgc tgcacgacgc cgccggccgc cacccgggcg
    21661 ggggcgagtt cagcggcgac cggctcgacc tggcggcgct ggccaagatc gcgcagcgcc
    21721 tgccgctgcc gccggcggtg cacgaacggc tgcaggcgca cccggtgcag ggcctggtcg
    21781 agcgcatcga ggcgcgctgg cagggcccgc tggaggctcc gaccgactgg cgcgtgcaga
    21841 cccgggtcag cggcctggcg ctggccgcgc cggcgcaggc gacacccgcc accggcgagc
    21901 cggtggccgg catccccggc atcgagggtg cggcgctgga gctggaggcc acgcccgccg
    21961 gcggccgcgc cagcctggcc atcgccgacg gcgcgctgac ctttcccggt gtgttcgaag
    22021 agccgcgcat cccgctggcg cggctcgacc tgcgcggacg ctggcgcgtg cagggcgagc
    22081 agctcgcggt ggaggtcgag cagctcgatc tgcgcaacgc cgacgccacc ggcagcttca
    22141 aggcgcgctg gcagacgctg gccggcgccc agggcgcggc gcgctttccg ggcctgctgg
    22201 acctgtcggg cagcttcagc cgtgccaacg gcgcgcgcgt gcaccgctac ctgccgctgg
    22261 tgatcccggc cgaggcgcgc cactacgtgc gcgacgccat cgtgcgcggt gacgcgcgcg
    22321 acgtggcggt gcgcgtgcgt ggcgacctgc gccagctgcc gttcgaccag cacccggagg
    22381 cgggcgaatt ccgcttcgcc gggccggtgc agggcgtcac catggcctac gtgccgcgcg
    22441 ccctgcagcc ggccgggcag ccaccctggc cggcgctgga ggggctggcc ggcgagctga
    22501 tcttcgagcg caacagcatg cgggtgcgcc aggccagtgc ccgcgtgcag ggccaccccg
    22561 gctggcgctt cacgcgcgtg caggccgaca tcgacgacct gcagcacgcc cgcgtgcggg
    22621 tcgaagccga agggcgcggc gtgctggcct cggcgctcga catcgtgcgc ggctcgccgg
    22681 tggccggctt catcgagcac gcgctggacc gtgccagcgc cagcggcgac gccagcctgc
    22741 agctcgggct cgacctgccg atcgacgcca tcgaccgatc cagggtgaac ggccgcgtcg
    22801 cgctggccgg caacgagctg cgcatcgccc ccgaggcgcc gctgctgacg caggcgcagg
    22861 gcgtggtgac cttcagcgaa accggctttg ccgtcgccga cgcccgcgtg cagctgctgg
    22921 gcgggcaggc gcggctgagc ggcggcagca ccgcggcggc gcagggtggc cccacggtgc
    22981 tgctgcgcgc cagcggcagc gccagcgccg agggcctgcg cgagatggcc gactgggccc
    23041 cgctgccggc actggcgcgc caggccaccg gcagcgccag ctacgaggcg cagatcggct
    23101 ttcgcggtgg cgagcccgag gtgcgcgtga ccagcgacct gcgcggcctg gccttcgatc
    23161 tgccggcgcc gctcgacaag ccggccgacg ccgcctggcc gctgcgctac gacagcggcc
    23221 cggtggccgg cagcgccggg cgccagcgcc tgcgtgtgga agtggcgcag cggctggcga
    23281 tcgaatacga gcatgccgcc gacagcgacc gcgtgctgcg cggcctgatc gggctgggcc
    23341 cgcgggccac ggcgctggcc ttgccggcca gcggcgtgca ggccggcatc gagctgccgc
    23401 acctggacgt cgaacgctgg cgcgacgccc tgcagcgggt gctgggcggc ggcgccacgc
    23461 tgtcggccga aggcggccgg ctgcccagtc agtggacgct gcgcaccgcc gagctggtgg
    23521 tcgacgggcg cagcctgaac gacgtcgttg ccagcggcac ccagcagggc ggccagtggc
    23581 gcggcgaggt gcaggcgcgc gagctggccg gccgcatcga ctttcgcgaa ggcgccgacg
    23641 gccgtgccgg caagctgtcg gcgcggctgg cgcgcctgag cattccggcc ggcggcagcg
    23701 acgaccgcac cctgctggcg cagccgccgg cccagatccc ggcgctggac gtggtggtgg
    23761 agcagttcga gctgcgcggc aaggcgcttg ggcggctgga aatccaggcc gtcaaccatg
    23821 acgtggcggg ccccgcagcc gccggtgaaa cgctgcaaaa ttgggagctg tcgcggctgt
    23881 ccctgcaagc gccagaggcc catctgaccg cccatggccg ctgggcggcg ctggcccgcg
    23941 gccccggcct gccgctggat ccgcgcggcc cgcgcgcgcc cgaggaccgg cgccgcacgc
    24001 agctggagtt caagctcgac atccgcgacg ccggcaagct gctggcgcgg ctggacatgc
    24061 ccgacgtgct ggcgcgcggg cgcggcgagc tgcgcggcgg tctgggctgg cgcggcacgc
    24121 cgttttcgcc gcactacccc agcatggccg gccagctgca cctggacgtc gacgccggcc
    24181 agttcctcaa ggccgacccc ggggtggcca agctgctggg cgtgctcagc ctgcaggcgc
    24241 tgccgcggcg gctgacgctg gacttccgcg acctgttttc gaccggcttc gcgttcgact
    24301 tcatccgcgg cgacgtcgag gtgcaggccg gcatcgcccg caccaacaac ctgcagatga
    24361 aaggccccag cgcggcggtg ctgatggacg gcgaggccga cctcgaccac gagacgcagc
    24421 gtctgcgcgt gctggtggtg cccgagatcg acgccggcac ggcggccctg gtggccaccg
    24481 ccatcaaccc ggccatcggc atcggcgcct tcgtggcgca actggtgctc aagcgcccgc
    24541 tcatcaaggc ggccacgcgc gagtttcaga tcagcgggcg ttgggacgac ccgcaggtga
    24601 cgccgcttgg cgcgccggcc gccgacgccc agacccccgc gccggcgacg ccgccgacgc
    24661 cgcgctcggc gaccgacacc cccgaggagg aacgctcatg aaagtcgccg ccatccagat
    24721 ggtctcgggc tcgcgggtcg aagacaacct gcaccgcgcc caggccctgc tggcgcaggc
    24781 cgcgcaggcc ggcgccgagc tggcggtgct gccggaatac ttctgcctca tgggtcagcg
    24841 cgacaccgac aagctggccc tggccgagcc gctgggcgac ggcccgatcc agcgctggct
    24901 ggccgacagc gcgcgcgcgc tcggcctgtg gctcgtgggc ggcacgctgc cgctggcacc
    24961 ggacggcgcg gcgccgccgc tcacccactg ccgcaacgcc acgctggtgt tttcgcccgc
    25021 cggcgagcgg gtggcggtgt acgacaagat ccacctgttc cagttcgacg acggcacgcg
    25081 ccgctacgac gaggcggcgg tgctgcggcc cgggcgtcag ccggtgcgct tcgacctgcc
    25141 gtcgcgcgac ggccaccgct ggcgcgtcgg tctgagcatc tgctacgacc tgcgctttcc
    25201 cgagctgtac cgccagcacg ccgccgacgg cgccgagctg ctgctggtgc ccagcgcctt
    25261 cacccacgtc accggtcagg cgcactggga ggtgctgctg cgcgcacgcg ccatcgagaa
    25321 cctgtgcggc gtggtcgccg ccgcccaggg cggcacgcac gacaacggcc gccgcacctg
    25381 gggccacagc atggtggtcg acccctgggg ccaggtgctg gcggtgcagg acgaaggcgc
    25441 ggcggtggtg ctggccgagc tggagccggc ccagctggcg gcctgccgcg cccgcctgcc
    25501 ggcgctgcgg catcgggtgc tggggtgagc gccacggcgc cggtgcgcgc ggccgccagc
    25561 cccgaccccg gcagcgccgc cgcgctgcgc cggctgcgcg ccggcgccgg gcggcgctgg
    25621 ctgctgtggg gcgtgctcag tctgacggtg gtcggcctgc tggcgatcgc cgtgtggctg
    25681 gcgcgcgggc acgagatcga acaggtgcag cgggcgctgg agcgcgacag cctggaagcg
    25741 gtgcaggtgc tgcgcgagcg cctggggcgc aacctgcagg acctgcacgg gctgggcgca
    25801 gcgcccgccg accccgaggc ctggagcgcc ggcgccgccc gcctgctgga gacgcaccgc
    25861 gagtggctgc ggctggagtg gcgcggcgcg gcgctggagc cgcgcgcggc gcgcgacagc
    25921 ccgttccaca ccccgctgcc ggccgaagtg cgcgaggcca tggcgcccga gctgcagcgc
    25981 ctgtgccagc aggccagcgt gcagggcgag gcgatctacg cgccgcccag cttcgtgctg
    26041 cgcgccggcg ggctggggcg cgaggtgatg acgctgtgtc tgccgctgca ggatggcgca
    26101 cggacccagg gcttcgtgct ggccagctac acgctggacg agctgctgcg cctgctgccg
    26161 accagcctga cgcgcggcca ggccgtggcc ttcaccgagc tggacggcac ccggctggcc
    26221 ctgcacggtg cggcgcggcg cggcgcgcgc ctgttcgtgg cgcagcagac gctggacctg
    26281 cccggcgcgc ccatgctgct gcggctggag ggcgggcggc cgctgccgga cgtgttcccc
    26341 aacgtgctca cggcgctggt gaccctgctg tcgatggcgc tgttgacggc gctgggcctg
    26401 ctggcgcgcg atatccgccg gcgccagcag gccgaggccg agctggccga ggcgctggcc
    26461 tttcgcaagg cgatggaaga ctcgctcatc accggcctgc gcgcgcgcga cctgcagggc
    26521 cgcatcacct acgtcaaccc ggcgttctgc gccatggtcg gctacacgcc gcaggagctg
    26581 atcggccagt ccacgcccgc gccctactgg ccacccgaac gcgcccacga atacggcgcg
    26641 cgccaggcgc tgcgcctgtc cggccaggcg gcggcgccgc gcgagggcgt ggaaaccgag
    26701 ttcatgcgca aggacggcac gcactttccg gtgctgatct tcgagacccc gctgatcacc
    26761 gcccagggcc agcacagcgg ctggatgggc gccgtggtgg acatgacggc gcagcgccgg
    26821 gccgaagaat cctcgcgcgc cagcctggag cgcctgcagg ccagcgcgcg cctggccacg
    26881 gtgggcgaaa tggcctcgct catgagccac gagctgaacc agccgctggc ggccattgcc
    26941 agctacgcca gcggctcgct caacctgttg cccgccggtg cgggcggcga cgctgccacc
    27001 gcacgcgagc tgcgcaccgc gctggagcgc atcgccgagc aggccgggcg tgccgggcgg
    27061 gtgatccaca gcgtgcgcga gctggtgcgc cagcgcagca ccgcgccgca ggcggtggcg
    27121 ccgcgcgccc tggtcgacgc cgtgctgccg ctggtgcagc tgcaggcgcg caagctgggc
    27181 gccagcgtgc gggtgcaggt ggcgcccgag ctgcccgccg tctggtgcga gccgaccatg
    27241 atcgagcagg tgctgctgaa cctggcgcgc aacggcctgc aggccatggc cgaggtgccg
    27301 ggcgagcgcc tgctgaccct gcaggcgcgc ctgccgaccg cgcccgccgg ccccgcactg
    27361 ctggagttcg gcgtcgccga caccggcacc ggcatggatg cggcggtggt cgaacgcctg
    27421 ttcaccccct tcttcaccac caaacccgag ggcatgggcc tgggtctgtc gctgtgccgc
    27481 accgtgatcg agcagcatgg cggcacgctg gtgcatgcgc ccaacctgcc gcgcggcacg
    27541 ctgttccgct tcacgctggc ggtggccgat gcgccatgac cagacgcccg gccgtggctt
    27601 cgtgcgccgc tgcccaagcg ctgtagggtc aggcgtcgtg cgtcatggcg tcggcgtgcg
    27661 ccgccagcag cgcccgatgg aatgccaacg ccgcgcgcgc cggctgcggc gagcggcgca
    27721 gcacgctgac gaagtcgcag tcgtagtgca gccgctcggg cgccacggcg cgcagctggc
    27781 cgcgctgcac gaaggcggcg gcgtagtggt cgggcagaaa accgatgaag cggcccgaca
    27841 ggatcagcgt ggcgatcgcc tcctggtcgt aggcggtggc gccgcgccgc agccgctggc
    27901 cgtgcgccag ctccatgttg ggtgagtggt agcccaggcc ggcaaagtcc tggcgcgccc
    27961 gcagggcagg ccagtccagc gccgaatgct ctgaatcgaa gagcgcatgg ggccgggcgc
    28021 agtacaggtg catggtctcg ccgaacagcc gctggtagcg caggctggcc gaggcgcggt
    28081 gcgccggaat cacgccgacg gcgaagctgc cgtccatcac cccgcgttcg atggtctgga
    28141 tgctgcccac gtgcaggtgc agcgccacgt ccggcgcctg ctgcgtgaag tcggcgatgg
    28201 ccgcgtggat gcgcgcctgc gggttgctga cggtcttgtc gaacagcgcc accgccagcg
    28261 tgccgcccat gcgctggtgg atgtcgtcga cgctggcgcg aaagccgtcc accgccgcca
    28321 gcaggcgccg cgtctcgtcg tacacgcgct ggccctcggg cgtgacggca aagccgccgc
    28381 gcccgcgccg gcacagcgtc aggcccaggc gggtttccag gtccttgacg tggcggctga
    28441 cggtgctggt gccgatgttg agctccagct cggccgccga caggccgccg cattcgacca
    28501 ccgccttgaa cacctgcagc aggcgcaggt ccatgtcgct cacctgaccc agcacggcgc
    28561 ggctgcgggc aggggtggga ttgacttgca tgatccggca actgaacttc gatatttgcg
    28621 tatttaaacg gctaacgacg gcgccgacaa tggcgttgtt ccgcccgtcc gggcccaagc
    28681 tgccgatccg aggagacccc ccatgagcgt cactgccacc gttcctgccc ccgccgccac
    28741 ccgccgcgac gccgcctggc tcgaggcgca ctggatgccc tttaccggca accgccagtt
    28801 caagcgcgac ccgcgcctga tcgtggctgc ccagggggcc tatttcaccg acgccgacgg
    28861 ccgccaggtg ttcgacggcc tgtccggact gtggtgcagt ggcctgggcc atggccggcg
    28921 cgagatcgcc gaggccgtgg gccgccaggc cgcgcagctg gactacgcgc cggcgttcca
    28981 gttcggccac ccgctgtcgt tcgagctggc caacgccatc aagcagcgca cgccccaggg
    29041 gctggatcac gtgttcttca ccgccagcgg ctcggaggcc gccgacaccg cgctgaagat
    29101 ggcgcgcgcc tactggcgcg cccggggcca gggcggcaag acgcgcctga tcgggcgcga
    29161 aaagggctac cacggggtca acttcggcgg catctcggtc ggcggcatgg tgggcaaccg
    29221 caagctgttc ggtcagggca tcgaggccga tcacctgccg cacacccagc cgccggccgg
    29281 caccttcgcc aagggccagc cggccgaggg tggggtggcg ctggccgatc gcctgctgga
    29341 gctgatcgcc ctgcacgacg ccagcaacat cgccgccgtc atcgtcgagc cgttttccgg
    29401 ctcggccggg gtggtgattc cgccggtcgg ctacctgcag cgcctgcgcg agatctgcac
    29461 ccagcacgac atcctgctga tcttcgacga ggtcatcacc ggcttcggcc gctgcggcgc
    29521 catgaccggc agcgacgcct ttggcgtggt gcccgacctc atgacctttg ccaaacaggt
    29581 caccaacggc gtgcagccgc tgggtggcgt ggtggccagc cgggcgatct acgacacctt
    29641 catggccgcc ggcggccccg actacatgct ggagttcgcc cacggctaca cctacagcgc
    29701 gcacccggtg gcctgcgccg ccggtctggc ggcgctggag ctgctcgagc gcgaagacgg
    29761 ccccggccgc gtgaaggccc tgagcgcgca tttcgaagcc gccgtgcaca gcctccgggg
    29821 cgcccgccat gtgctggaca tccgcaacta cggcctggcc gccggcttca ccatcgcccc
    29881 ggtggcgggc gagccggcgc gacgcccgta cgacatcgcc atggcctgct ggaagaaggg
    29941 cttttacgtg cgctacggcg gcgacaccat ccagctggcg ccgcccttca tcagcgaaaa
    30001 ggccgagatc gagcgcctgg tgaacgcgct gggcgacgcc ctggccgaag tggcctgagc
    30061 gggcgggtgg ttccgggcct gacggatgac gaaatgacct cgctgcgcgt cgatgaccca
    30121 tgggtcatgg gtcgttggcg gggatggcgc ctggcgttcg aggtccggaa actcctattt
    30181 tgatagctgc cagcgcttgc cagtaaagcg ccagagcctg atttgccatc aaacccgggg
    30241 ccaaccacgg aggccggccg cgccttggcg tgctggccga cacgcgcgcg atggtcgatg
    30301 cgccgcaggg gcgtgcgacg ctccggatgg ttgcgtccag ccccgcccgc ggatggcgcg
    30361 ggacgcggca gcccgtgccg tcaggccggc acgtgccgcg gcagcgagcg cgggccggcc
    30421 tggctgcggc cgtagcgctc caggcccagc ccggcggtcg agatgtcgct ctgacgcccc
    30481 agcgtcaggt cggccaccag cttgcccgag ccggccgcca tggtccagcc cagggtgccg
    30541 tggccggtgt tgagcagcag gttggcgtag cgcgtggcgc cgaccaccgg cgtgctgtcg
    30601 ggcgtcatgg ggcgcaggcc ggtccagaac tcggcgcgcg ggatgtcgcc gccggtgaac
    30661 aggttgccca gcaccagctc cagcgtttcg cgccggcgcg ggttcaggcg caggtcgaag
    30721 ccgcccagct cggccatgcc gccgacgcgg atgcgctggt cgaagcgggt gatggcgacc
    30781 ttgtaggttt cgtccatcac ggtggagcgc ggcgcgttgg ccgggtcgag cagcggcacg
    30841 gtgagcgaat agcccttgac cgggtacacc ggcagatcga gcccca
//
LOCUS       DAOOOG010000012        30022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_1339_length_30022_cov_24.226182, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000012 DAOOOG010000000
VERSION     DAOOOG010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 30022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30022
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1339_length_30022_cov_24.226182"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PK242_02085"
     CDS             complement(<1..923)
                     /locus_tag="PK242_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ92842.1"
                     /translation="MSLSVPPAQLVHFSTRDVEPGLRRQAWHEIAHRWVDFRPAPDVP
                     LDAELTILSSDVCTLGATRSSAYEMRTGSRRAERDDMVVLTLMQSGELVPEAFSRKGA
                     GALSLCVPREMGAFLWGQDARQAFLTLPRSDVVAALGHEPRTALLTTHCALAPALASQ
                     MNHVALLLGQSGRVDAEEYAGLLDATRALALLALRNLGRQGAVVDLPDRTESLHAGRR
                     AAALRFMELHAHRHELDAAAIARGAGCSRTRLYEAFAAQDQTVMGVLRELRLQRARTL
                     VERGAQLHVGALSWRCGFADQSAFSKLFRAR"
     gene            1139..1894
                     /locus_tag="PK242_02090"
     CDS             1139..1894
                     /locus_tag="PK242_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92843.1"
                     /translation="MSTRRFLRTLTASVALLAGAHAAAAPYATTYTGAIANSQITANA
                     PDGAAFTLTLVFDNGGSSAASQNWDIGQLTCGFWRWRVDDTRSVTVALDMSGGLAQGA
                     GSVSTNAAGELTAVYASVDTGGPLAWADYDVSGLPAGSAIGWFADGNPQVFGLMANGG
                     GGSFDDGTGPGGGVQMTPGRWQAPLPFTGACDASAVPPTPPGPPAPVAVPTLPWWGLV
                     VLGALLARVATRRRFLNHLRLKRPTSQRQQLSH"
     gene            1914..3239
                     /locus_tag="PK242_02095"
     CDS             1914..3239
                     /locus_tag="PK242_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOZ92844.1"
                     /translation="MQDPSLHTPHRRHFMAIAASLALPVAAQDKPASKLERGSTRVAG
                     FADAEMDFQLMRQLGTARYGGASVGECFALARRIQSGMPASWVAAFSAAAARQEADAR
                     QRAQRGHALSAREQYLVACNSYRAAEYYSGVHSPEHARLGLKSRECFLAALRGADAGV
                     EEVSLPWGDTPLPAYYVRSGATRGRVGKTLMIISGYDGTLEETWLSYGRAALERGYHL
                     MLFAGPGQMDTLRFHPRMAFVPDYERIGRLALEHVLKRPETDPDRIALMGISYGGYFA
                     TRIAAHEPRVRALIANSPIIDLHAYMTSFVGFDPSDMPDDQDVRLADIDQIPDAAIPP
                     QTREMMRSLMLRMGQGSFKQTYQRLREFRVDDDGLRRIRCPALALVGTGEGAEPLAQA
                     ERFVQTVGGPVTRHVFTAEEGADGHCQTGNLAYSAAVSMDWLDELFDKA"
     gene            3236..4819
                     /locus_tag="PK242_02100"
     CDS             3236..4819
                     /locus_tag="PK242_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOZ92845.1"
                     /translation="MKRDQTMRPGLARLLRLAAAALATAAAVACGGGDAADASVRQAA
                     AQLDTLVPQWMARTGVPGVAVSVVHGGRTVYARGFGVRRVDEAARVDADTAFPLASVS
                     KSLAATVMAARIPPDAGWDTPIQQLLPTFGLAYPDAQDNARLTLGDLFAHRSGLPDHA
                     GDQLEDLGYGRTEVLARLRHARLNPYGSYAYTNFGLTAAAEALARSLGTDWATLSEQA
                     LYQPLGMTHTSSRHADLQARSNRAWGHVQANLSYDSYGTQPARYVVQQPQRQPDAQSP
                     AGGASASAADMARWMALVLAGGRWQGQQLLDPVALQAALAARPGGTYGYGFNVGPDPH
                     GHPSVSHSGAFLLGAHTAFILWPEAKLGITVLTNAQPRGLAEAIALAFGELALGDAAD
                     GVLTADWLAVMQGRMRGLYQPLGQYAGQPPPETPAPPQPLARYAGRYTNAYYGSAQVT
                     PNADGKTLDLVLGPAKVLHRLRHWSGDSFVFELQGENAPPGSVSAVRFAPDERAYMQI
                     EYYAEDLTRGRFDRDADTP"
     gene            4896..7007
                     /locus_tag="PK242_02105"
     CDS             4896..7007
                     /locus_tag="PK242_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92846.1"
                     /translation="MPISSAHCCRPTPVAAALALALTLALTACGGDDDPISGTPPPGS
                     GDPAANKSIVCKDYDNQDVLVKPKTITIRNNSEGPIYPVLATSMNLKNEWVMACQRTN
                     ELIATDAVYKLYVNDGQGIPPGSEVTLTLPLYSELSPGKYVTWWNGGRVLLADRNKRL
                     RGSVDKPTNTPPSVDCKGTGTTCNLSTYTSPVQFQEDVFAQLSEYTFGDSIIPPGQST
                     RLLKPENVGYNISYVDHVYMPVAIGVRGNPYIGYSGSARKLSDFRATLRSFVQPGSLG
                     EGWPLYNMSELRLPGGYNIFAQRGGYLLEDPDVPVKPADGKNPPVLTVLKCLEKDVDC
                     ATPEAQRNMQWGQAVQNIQDLWGSCVDWGSEDISQYTRKKYPQDCPAPQAMREELALI
                     KDFFAQNHKQYLAMYANQQCVGDPEGSRKIPAHVAEFKYWEAIKHIYGWVPFNEGCGA
                     DANPLAKTTVHGRDHAYVQTLYIEDLQYNYRQAAVQANPKLAFNPYVKLIHDDLGMSA
                     YGFSVDDAVGFMSEQGDGLVFTVGGTEGLENDQAFNYADGFSLGLGVPLEIDGKLNIQ
                     MIKKYGVCSLGAEPADPNCEQNKQDMIMPVGRLVSGFRVGTVASYPLKVRFTDMEDNV
                     YTVLVREKFAKCTGPLSACPSNRAQIVDKAACSVITPQGTKHPQSDTWCANANPNQAR
                     DNEQAVVKNHLSYPKPVKWLP"
     gene            7110..>7554
                     /locus_tag="PK242_02110"
     CDS             7110..>7554
                     /locus_tag="PK242_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92847.1"
                     /translation="MKRTSIGLAAVAALALPLAAQAATQTFTTPGSHSWTVPPGVTHI
                     RVQALGGGGGGGDGVANDGQSASGGPGGLVTSGNLSVTPGQTLTIVVGGGGGASGELE
                     GMGQGGGGGGASSVQVGSTWIVAGGGGGGSYYNDNSVSDTGGWGQG"
     assembly_gap    7555..7654
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7655..10431
                     /locus_tag="PK242_02115"
     CDS             <7655..10431
                     /locus_tag="PK242_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92848.1"
                     /translation="LAAHAAMDDEDFERYLLTAGKGTPITPETIKSLSVGSSINSAQS
                     TYNLRPIFDLGRLKEDGRLNVDTTRSQQKTVLRVTSNKAASTSAEQLNLDVSASGSVG
                     RESFGAKASAAVHHANTRRDTKSDGTVSVQMVSANTNNLVSILSSGFSGSENFTGYLI
                     GTKLTDEQLRGYVATTESTPTGICGGKPYTTSVKVSRYKLSDTDPYANIQILTRMENV
                     FADLKAQYELCADASIQTVLVRQMTELRGKIESAIADFYAFNGDAFVSQTTSMNQAIG
                     SGTMSFTTKDGNAEEQYGASVSAQYQTLTSGVGGSVTVQYYKQHGWAEALLNVQVSAE
                     AKPAGVADTTAWVSSIQTMLKDQGSSLVPPMGNLPKDPGVKLPEPVGLRHDEEAPPDV
                     AFTSYDQWKQYQQDKKDKKGVKDEVVLEQVQQQIENEPLRINDDNPQLKATQGGDAYE
                     QLIAELDALKERATAPQAPPQGNDGNLVRFDKMFVSGFETLPYDAVIPQLRPDLDIPG
                     IDQGMGSFPNIMTAMLMSEKLGRLDSYLAFLANISVSNVTPEMSERYHQFFQKASARA
                     YDLVGLALSQGTDMTPAVLAGYKKAMLGTASAPTQSELYKSLQDIDYYNYVVDTLLDP
                     ARGKAWFVAPGGYIPMRWKNDDRGGAELVTWTGLAPARGIAPGKESVVAVDFNNPNAD
                     PLTLYRNGMKILQTPWYPVYVFNQGQAPALVFVQHFGAYQAIYGARWVTRPYDGDNPV
                     KPSLVPQWQDYQPLGTRDMRTVMTGANNSFLGQFMNWDYSVYFPNSSNDPVRLRKYNA
                     LVLHLPADYQESDGQTMYTAAHSAAVRDFSHLAYDTQSRTDATYNAFRQPTHGRKMRR
                     MSDGAVVDTTHWTLGNGALTSVMLLPLNTTTTSESLTQGLNYAPQRAPMDVVTPTSLD
                     LVNKLAVLLE"
     gene            complement(10525..11940)
                     /gene="dnaB"
                     /locus_tag="PK242_02120"
     CDS             complement(10525..11940)
                     /gene="dnaB"
                     /locus_tag="PK242_02120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOZ92849.1"
                     /translation="MSSVFPPGYADLGDDAPTDRQVAQLRVPPHSHEAESSVLGGLLL
                     DNGAWDRVGDVLVDADFYRFEHRLVYGAIGKLVAESKPADVITVHEELQRQGKGDEVG
                     GLAYLNSLAQYVPSASNIRRYAEIVRERSILRQLVSASDDIATSAFNTQGRPVAKILD
                     DAEGKIFKIGEQSSRMKQGFQAMDSLVVQLLDRVQEMADNPNDVTGVPTGFIDLDRMT
                     AGLQPGDLVVLAARPSMGKTAFAINIAEHVALHEQLPVAVFSMEMGASQLAVRIVGSI
                     GRIDQGHLRTGKLTDEEWPRLTEAIERLRNVSLHIDETPGLTPSELRANARRLARQCG
                     KLGLIVVDYLQLMSGSSSGDENRATELGEISRGLKALAKELQCPVIALSQLNRSVEQR
                     TDKRPMMSDLRESGAIEQDADIITFIYRDDYYNKDSREPGVAEIIIGKQRNGPTGTVK
                     LAFIKPLTRFESLAMGSSDDY"
     gene            complement(12134..12586)
                     /gene="rplI"
                     /locus_tag="PK242_02125"
     CDS             complement(12134..12586)
                     /gene="rplI"
                     /locus_tag="PK242_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOZ92850.1"
                     /translation="MQIILLDKVTNLGNLGEIVKVKDGYARNFLIPSGRARRATEAAK
                     AEFEARRAELEKQAAEKLAAAQAVGEKLNGQTIKLTQKAGVDGRLFGSVTNHDIADEL
                     KKQGYDVHKSQVRLPAGPIKTVGDNTITVSLHTDVSAEITVSVYGETA"
     gene            complement(12602..12883)
                     /gene="rpsR"
                     /locus_tag="PK242_02130"
     CDS             complement(12602..12883)
                     /gene="rpsR"
                     /locus_tag="PK242_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOZ92851.1"
                     /translation="MATFKKFNKDKRPKRNTQSLLFRRKRFCRFTVAGVEEIDYKDVD
                     TLRDFIAENGKIVPARLTGTRAIYQRQLNTAIKRARFLALLPYTDQHRV"
     gene            complement(12887..13189)
                     /gene="priB"
                     /locus_tag="PK242_02135"
     CDS             complement(12887..13189)
                     /gene="priB"
                     /locus_tag="PK242_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HOZ92852.1"
                     /translation="MNQLRLQARIVEIGALRHTPAGLPALDLRLEHESTQQEAGQSRQ
                     VRAALRAVALGAMAERLGRQVIGSQACFSGFLASPQRTRSVVFHIQDFQQDQFQGA"
     gene            complement(13221..13583)
                     /gene="rpsF"
                     /locus_tag="PK242_02140"
     CDS             complement(13221..13583)
                     /gene="rpsF"
                     /locus_tag="PK242_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOZ92853.1"
                     /translation="MRHYEIILLIHPDQSEQVPAMLERYKGMITAGGGKIHREEDWGR
                     RQLAYQINKLAKAHYLCLNIEADQAVLGELEHAFRFNDAVLRHLTVQKKKAETAPSSM
                     MKTVEREEARKAQQAEAN"
     gene            complement(13737..14534)
                     /gene="ybgF"
                     /locus_tag="PK242_02145"
     CDS             complement(13737..14534)
                     /gene="ybgF"
                     /locus_tag="PK242_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOZ92854.1"
                     /translation="MAIFRVTSLRAAVLALACLAGQGAQAQLFGDDQARQAILDLRQR
                     FDQAVAAQNRLVDENAQLRRGMLDLQQQIETLRAELARARGQDEQLARDLADLQKAQS
                     TQQQGWDERLRKLEQEASARMSDPNAAAGAPPVASDGAAASEKQDYDAALAVFRAGDF
                     KGAQTGFANFIKRYPRSTLAPSALFWLGNAQYATRDYKEAITNFRSLLTAAPSHARAP
                     EAMLAIANCQIELKDTRAARGTLESLIKTYPQSEAAQAGKDRLARLK"
     gene            complement(14542..15081)
                     /gene="pal"
                     /locus_tag="PK242_02150"
     CDS             complement(14542..15081)
                     /gene="pal"
                     /locus_tag="PK242_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOZ92855.1"
                     /translation="MFKRYLMVAALAAVLAGCGSNVKLDPPIEGSASAGASAAGQGGA
                     SGQGAGQSTVTQVQAGAAGADAAGPNGVGRVIYFDFDSFVVKSEYQSLVDQHARFLQA
                     NRGRSVALEGHTDERGSREYNLALGQKRAEAVRRALTLVGASDAQVEAVSFGEEKPAA
                     AGASEDAYAKNRRVEIRYR"
     gene            complement(15141..16442)
                     /gene="tolB"
                     /locus_tag="PK242_02155"
     CDS             complement(15141..16442)
                     /gene="tolB"
                     /locus_tag="PK242_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HOZ92856.1"
                     /translation="MKSALLDKFSLAPWQRRGFVTALAAAPFSAALAQFRVEVSGVGL
                     TQLPIALVPFRGEDAAPQKISAIVRADLERSGQFRAVDAGGQSMDETTRPEMSLWRQR
                     TADALAGGSVTRLADGRWDVRFRLWDVVRGQDLGGLGYVVPQADLRLAAHKIADYIYE
                     KLTGDKGVFSTRLAYVTKGGSRYTLWVADSDGENAQAALGSPEPIISPVWSPDGGQLA
                     YVSFESRKPVIYVHDVASGRRRLLANFKGSNSAPAWSPDGRSLAVTLSRDGGSQLYLI
                     SASGGEPRRLTQSQSIDTEPAFSPDGASIYFVSDRGGAPQIYRMPVGGGAAQRVTFSG
                     SYNVSPAISPDGRYLAYVSRTNGYRLHVMELASGTTTAVTDTAEDESPSFAPNSRLIV
                     YATRQGGREALMTTTLDGKIKARLAGQGGDIREPAWGPYQR"
     gene            16662..18479
                     /gene="msbA"
                     /locus_tag="PK242_02160"
     CDS             16662..18479
                     /gene="msbA"
                     /locus_tag="PK242_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HOZ92857.1"
                     /translation="MAAPPAPAPPASPSAQQPLGRRLARLWTYFREYRLGWLIAALGT
                     LVSALTEASVPALLKPLLDEGFTQGSLPIWVVPLAIVGLFALRGFAHFASQYALARIA
                     NDGMVKLRRQLFDRLLVAELSLFARQSASQLANTLVYEVQTGTTLLVQALLNLARDSF
                     SVVALLAYLLYLNWSLTLIVLVLVPIVALIMKTMSRRLYRYTRLSQTTTDELAYVVEE
                     NVLAHRMVRLHGAQGQQAGRFDTLSRQLRGLSIKATSASAAAMPLAQMAAAVALSVVI
                     GIALHQGQSGAGQRITVGGFVSFIGAMLMLIQPLRRLTDVVGPVTRGLAALERGLDLM
                     AQVQPEAGPAATATSAASRALGELSLREVTVTYHGAAEPVLQQLSLHVRAGEMVALVG
                     PSGAGKTTLINLLPRFVRPSSGQVLLDGQDIADWPLAALRAQFALVSQDVTMFNDSIA
                     ANVALGAPSIDRERVQRCLQDANLADHVATLPRGIDTVLGHNATELSGGQRQRLAIAR
                     ALYRDAPVLLLDEATSALDTESERLVQQAITRAMAGRTTLVIAHRLSTIEHADRIVVM
                     ERGRIVEQGSHAELTAAGGLYARLHGQPSASDAGSPGNS"
     gene            18724..19959
                     /locus_tag="PK242_02165"
     CDS             18724..19959
                     /locus_tag="PK242_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOZ92858.1"
                     /translation="MSPHSALRLHVPEPTGRPGAKTDFSYLSISPAGAVRQPPIDTAA
                     ADTADLATSLVRVLDSEGRAVGPWAPAIHPERLRRGLRAMLKTRLFDARMLLAQRQKK
                     LSFYMQCLGEEAIAVAHAQALREGDMCFPTYRQQGLLLSRDDISLVELICQLMSNERD
                     PIKGRQLPVMYSYKRAGFFSISGNLATQMPQAVGWAMASAIKGDTKIASAWIGDGSTA
                     ESDFHTSLTFAHVYRAPVIINVVNNQWAISTFQSIAGGEGTTFAQRGVGVGIASLRVD
                     GNDFLAVYAASQWAAERARANHGPTLIEWETYRAGPHSTSDDPSKYRPADDWQRFPLG
                     DPIERLKQHLIAIGEWSQAQHEATHKALEAEILAAQKEAESHGSLLDGRVPSAATIFD
                     DVYRDLPEHLRRQRQQMGV"
     gene            19963..21003
                     /locus_tag="PK242_02170"
     CDS             19963..21003
                     /locus_tag="PK242_02170"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOZ92859.1"
                     /translation="MEQAVQHATATPMTMIAALRSAMDVMMARDDNVIVYGEDVGYFG
                     GVFRVTEGLQAKYGKTRCFDAPISESGIVGTGIGMAAYGLRPVVEIQFADYVYPATDQ
                     IVSELARLRHRSAGDFSAPMVIRMPCGGGIYGGQTHSQSPEAFFTHVCGLRTVMPSNP
                     YDAKGLLISAIESDDPVIFLEPKRLYNGPFDGHHDRPVVPWSKHELGKVPEGHYRVPL
                     TSAAIFRPGKAVTVLTYGTMVWVSECAARETGIDAEIIDLRSIWPLDLDTIVESVRKT
                     GRCVVVHEATRTSGFGAELVALVQEHCFWQLEAPIERVTGWDTPYPHAQEWAYFPGPG
                     RVGEALKRTLEV"
     gene            21007..22305
                     /locus_tag="PK242_02175"
     CDS             21007..22305
                     /locus_tag="PK242_02175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOZ92860.1"
                     /translation="MGTYVIRVPDIGEGIAEVELVAWHVQPGDAVSEDQHLADVMTDK
                     ATVEVPSPVAGKVLTLGGGVGQVMAVGAELVRLDVEGAGNFKENEASAPAPSAPAAPV
                     SIATEVIAQAPDAAPRAPAAAAARGSVAAPRPVAGRRKEGERPLASPAVRRRALDLGV
                     ELRFVHGSGPAGRIGHQDLDAYVAGQGQAMLTGASPYAERHGEEAVPVIGLRRKIAQK
                     MQEAKRRIPHFSYVEEIDVTELEALRQQLNQRHGATRGKLTLLPFLARAMVCSLRDFP
                     QINARYDDDAGVVTRHAPVHLGVATQTDGGLMVPVLRHAESLDLWAFAAGLTRVTEAA
                     RTGKAAREELSGSTITITSLGPLGGVVTTPVINHPEVAIVGVNRIIERPMLRGGQVVA
                     RQLMNLSSSFDHRVVDGMDAARFIQAMRALLETPALLFVE"
     gene            22315..23715
                     /gene="lpdA"
                     /locus_tag="PK242_02180"
     CDS             22315..23715
                     /gene="lpdA"
                     /locus_tag="PK242_02180"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOZ92861.1"
                     /translation="MTKEVTTKLLIIGGGPGGYVAGIRAGQLGIPTMLVEGASLGGTC
                     LNIGCIPSKALIHAAHEFDQARAQATGSPTGIRVQRPQIDIAQTVRWKDGIVQRLTGG
                     VGGLLRRAGVQVLKGWAEIEDGKTVTVKPHQGEPMRVRCEHLLLATGSEPVELPSMPF
                     GGAIWSSTDALSPDLLPRRLVVVGAGYIGLELGMAYRKLGVDVTVVEAAQRVLPSYDE
                     ELTEPVLQALQKQGVVLHLGCSVAGWDAQHGVHVRIDARAEDFALPADRVLVAVGRRP
                     RTAGFGLESLQLDMAGRHIAIDAHGRTSMRNVWAIGDVTGEPMLAHRAMAQGECVAEQ
                     IAGRNRRFEPMAIPAVCFTDPEVVVTGKTPGEARAAGMDCIDAAFPFAANGRAMTLEA
                     ASGFVRVVARRDNHLILGWQAVGKGVAELAAAFSQSIEMGARLEDVGHTIHAHPTLGE
                     AVQEAALRALGQALHI"
     gene            complement(24091..25122)
                     /locus_tag="PK242_02185"
     CDS             complement(24091..25122)
                     /locus_tag="PK242_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOZ92862.1"
                     /translation="MTLQATVSMSWVDAVLAEAERQGVGRGRLLAQAGIAPEELQQER
                     WPIDHITRLWHAAVACTQDAGFGLAAGSRAGPDNFNVVGELLRASATLREAIAAVQKY
                     QRLISDGGRFQMVAGARASWLIYHPRQGTLAFSPHQVEAVLATVLAFVRNALGQRPGA
                     GVRPLQVQFSQPRVGPLAGYREVFACPLAFEQAFNGLLLDNAVLDAPLPHADPHRARA
                     HQREAAAHLAGLSQGGALAQELRAWMAAALASRVPTRAEAAAALGVSERTLARRLRAQ
                     GISFAELLDGVRRDAALQAVGQTTRPLADIGQALGFAEPAVFWRAFKRWSGSTPAAWR
                     AAQRADRQP"
     gene            complement(25133..26146)
                     /locus_tag="PK242_02190"
     CDS             complement(25133..26146)
                     /locus_tag="PK242_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOZ92863.1"
                     /translation="MDSATQLILGAAVGVAVMRRRTAAWKAALWGGVAGTLPDLDVLI
                     AHGDPILNMVLHRAESHALFWLTLFSLPFAWGVARVQGEWGQWRRWWLALWLALVTHP
                     LLDAFTVYGTQLLLPLTDYPVALGSVFIIDPAVTLPWLVGLVVVLARRGDVRGQRANL
                     WGLALGTAVLLAGVGLQARVEHLARASLAAQGVHPERVLVTPAPLSMLLWRVVAMEGD
                     GYYEGFHGLLDGPGPMRFERFDRGARLEAAVAAIDGAQRIRRFSHGFYKLHESAGGQL
                     VITDLRMGLEPHYSFAFAVAQRGSPFAPLPVAQTVGGAVPVGPALAWLGRRMRGEQVA
                     PPR"
     gene            complement(26538..27863)
                     /locus_tag="PK242_02195"
     CDS             complement(26538..27863)
                     /locus_tag="PK242_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92864.1"
                     /translation="MTKHLLILGAGVDRTAGIDFPLANTLLADVTRFLDGPGKDVETA
                     LRDMLPGLRFSFNSMIARAVDKIATREPHEQKAMVQRVQDAIAHLGEDQEGIRKHGEL
                     IIRLFNKLATIAEESQLDADTEALIREVFPKDADELIDSDSILDIHKLSLSDTFKTVL
                     KRTLKLGLSSERHEVAAALGADMLNIETLLIEKFLGFYNDKPADIKNYLYIAWALWAY
                     LVAKQKEVLEAHAASPLPFYGKVPDDLRAITLNYTAFLEQSLGAAKTIYFHGGLSDYV
                     RMDTRDLLPVDDILNCDPAKFIREVVTPNVDVSADDLRQQRHVIPALVPPLRLKPILS
                     HRYIELWSQASEWVKQAEHVIVVGYSFNNADEHFNDILRCHPDRRIDIVVPEATSTHF
                     LARMEKVFGTAANQYNKIKVNGFDALKAKKVRLIAAKAGEVNLSELFAN"
     gene            28120..29694
                     /locus_tag="PK242_02200"
     CDS             28120..29694
                     /locus_tag="PK242_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ92865.1"
                     /translation="MDPFGIVALVVAGGWLIGKLNDDNKPKPPPPSGRAPPSPTSSPG
                     ARGVYHPVAPDRVHRPAKQAQAEDAIEVLPEYKLVRDLVRAGFPITFVTGGAGTGKST
                     FVHWLTSQFNGTVLLTAPTGIAALNINGKTLHSLCKLPPAWITKRDIRYAGRDIVQHA
                     QLLIIDEISMVNANLLDAVSAFFCKNRDSDAPFGGLPVVLVGDLFQLPPVVNGQTRHL
                     FEKHYPTSKFFGARLLRDAPYYALELHKAFRQADQTFVDLLAKLREGVDVADSIAELN
                     RLCTITDRPPDQAVWLSPRNKEVDARNWAELRRLPAPSRFYQGKLTGRFRDDRLPSPK
                     RLELRIGAQVMFTKNGGRWVNGTIGVVVGLLDGRVEVRLHETGTVVDVGPVTWEQFDY
                     HLNQQTNDIERTVVGEYQQIPLMLAWSVTIHKSQGKTIDRVHIDLGAGAFETGQTYVA
                     LSRCRSLSALTLSRPLRESDVLVDAESRQFYGKLRELIRTVPPQAMAQKMGVAWGNTP
                     APAPASRKCLPLANKA"
BASE COUNT         5092 a        10439 c         9754 g         4637 t
ORIGIN      
        1 aagcgggcgc gaaacagctt gctgaaggcc gactggtcag cgaagccgca gcgccaggac
       61 agcgcgccca catgcagctg cgcccctcgt tccaccagcg tccgggcgcg ctgcaggcgc
      121 agctcgcgca gcacgcccat cacggtctgg tcctgcgcgg caaacgcttc atagaggcgg
      181 gtgcgcgagc agcccgcgcc gcgcgcgatg gcggcggcgt ccagttcatg gcggtgggca
      241 tgcaattcca tgaagcgcag cgcagcagca cggcggccgg cgtgcaggct ttcggtccgg
      301 tcgggcaggt cgacgacggc gccctggcga cccaggttgc gcagcgccag cagcgccagc
      361 gcgcgcgtgg cgtccagcag gccggcgtac tcttcggcat ccacccggcc gctttgcccc
      421 agcagcaagg ccacgtggtt catctggctg gccagcgccg gagcgagcgc gcagtgcgtg
      481 gtcagcagcg cggtgcgtgg ctcgtgcccg agcgcggcga ccacatcgct gcgcggcagc
      541 gtcagaaagg cctgacgcgc atcctgcccc cagagaaacg cgcccatctc gcgcggcacg
      601 cacagactca gggcgcccgc acccttgcgg gaaaaggctt cgggcaccag ttcgcccgac
      661 tgcatcagcg tcagcaccac catgtcatcg cgttcggcgc gacgcgagcc ggtgcgcatt
      721 tcgtaggccg acgagcgggt ggccccgagg gtacacacat cgctggacag gatggtcagc
      781 tcggcgtcca gcggcacatc cggcgcaggc ctgaaatcca cccagcggtg ggcgatctcg
      841 tgccaggcct gccgacgcag gccgggctcg acatcgcggg tgctgaagtg gaccagctgg
      901 gcaggcggca cagacaggga catggcctcc attgtatgaa cgcgggacgc atttcccaca
      961 cctgggacgc ctgcacccaa ggtcgccgtg gcggcgcatc ggtccgtaac tagacttttc
     1021 cacacccctt ttattgcaac cggcgccgga tctcggcaat gcatgtcaaa ggctccgtcg
     1081 ccggattggc cggcgcaggc agccgtgtgc gcgagcgggc tctgacggaa cgaactggat
     1141 gtccacgcgg cgatttctgc gcaccctgac ggcctcggtc gcgctgctgg ctggggcgca
     1201 tgcggcggcg gcaccctacg ccaccaccta caccggcgcc atcgccaact cgcagatcac
     1261 cgccaatgcg cccgatggcg cggccttcac cctcaccctg gtgtttgaca acggcggcag
     1321 tagcgccgcc agccagaact gggacatcgg ccagttgact tgcggcttct ggcgctggcg
     1381 cgtggacgac acccgcagcg tgaccgtggc gctggacatg agcggtggcc tggcgcaggg
     1441 tgcaggcagc gtcagcacca acgcagccgg ggagttgacg gcggtgtatg cctcggtcga
     1501 taccggcggc ccgctggcct gggccgacta cgacgtcagc ggcctgcctg cgggctcggc
     1561 catcggctgg tttgccgatg gcaacccgca ggtctttgga ctcatggcga acggaggcgg
     1621 cggcagcttc gacgacggca ccggccccgg cggcggcgtg cagatgaccc ctggccgctg
     1681 gcaagcgcca ctgcccttca cgggtgcctg cgatgccagc gccgtgccgc ccactccgcc
     1741 cggcccgccc gcgccggtgg ccgttccgac gctgccctgg tggggcctgg ttgtgctcgg
     1801 agccctgctg gcgcgggtcg ccacgcgccg caggtttctg aaccatttac ggctgaagcg
     1861 ccctaccagt cagcgccaac agctatcaca ttaagagcaa ttcgagacct gccatgcaag
     1921 accccagcct gcacaccccg caccgtcgcc acttcatggc catcgccgcc agcctggccc
     1981 tgcccgtggc cgcgcaggac aagcccgcca gcaagctgga gcgcggcagc acccgcgtgg
     2041 ccggatttgc cgatgccgaa atggacttcc agctgatgcg ccagctggga acggcgcgct
     2101 acggcggcgc gtcggtgggc gagtgcttcg cgctggcgcg gcgcatacag agcggcatgc
     2161 cggccagctg ggtggcggcg ttcagcgccg ccgccgcgcg ccaggaggcc gacgcgcgcc
     2221 aacgcgcaca gcgcggccac gccctcagcg cgcgcgagca atacctggtg gcgtgcaaca
     2281 gctaccgcgc cgccgagtac tacagcggcg tgcacagccc cgaacacgcg cgcctggggc
     2341 tcaagagccg cgaatgcttt ctggccgccc tgcgcggggc ggatgcaggg gtggaagaag
     2401 tctcgctgcc ctggggcgac acgccgctgc cggcgtacta cgtgcgctct ggcgccaccc
     2461 gaggccgcgt cggcaagacg ctgatgatca tcagcggcta tgacggcacg ctggaagaaa
     2521 cctggctgtc ctacggccgc gccgcgctgg agcgcggcta ccacctgatg ctgttcgccg
     2581 gccccgggca gatggacacg ctgcgctttc atccccgcat ggctttcgtg ccggactacg
     2641 agcggattgg ccggctggca ctggagcacg tgctcaagcg cccggaaacc gacccggacc
     2701 gcatcgcgct gatgggcatc agctacggcg gctacttcgc cacccgcatc gcggcgcacg
     2761 agccgcgcgt gcgcgccctg atcgccaact cgcccatcat cgacctgcac gcctacatga
     2821 cctcattcgt cggttttgac ccgtccgaca tgcctgacga ccaggacgtg cgcctggccg
     2881 acatcgatca gattccggac gcggccattc ccccgcaaac ccgcgaaatg atgcgcagcc
     2941 tgatgttgcg catggggcag ggcagtttca agcaaactta tcagcgcctg cgcgaatttc
     3001 gcgtagacga cgacggcctg cgccgcatcc gctgccccgc gctggccctg gttggcaccg
     3061 gcgaaggcgc tgaaccgttg gcgcaggccg aacgcttcgt gcagaccgtc ggtggcccgg
     3121 tgacgcgaca cgtcttcacc gccgaagaag gcgccgacgg ccactgccag accggcaacc
     3181 tggcctactc cgccgccgtg tcgatggact ggctggacga gctgttcgac aaggcatgaa
     3241 gcgcgaccaa accatgcgtc ccggtctggc ccggctgctt cgcctcgctg ccgcagccct
     3301 ggccacggcg gccgcggttg cctgcggcgg cggcgatgcc gccgatgcct ccgtgcgcca
     3361 ggccgccgcg cagctcgaca ccctggtgcc gcagtggatg gcgcgcaccg gcgtgcccgg
     3421 tgtggccgtg tcggtggtgc atggcgggcg cacggtctac gcacgcggct tcggcgtgcg
     3481 ccgggtggat gaagcagctc gggtggatgc cgacacggcg ttccctctgg cttccgtttc
     3541 caagtcgctc gctgccaccg tcatggccgc gcgcatacca cccgatgcgg gttgggacac
     3601 gcctatccag cagcttctgc ccacattcgg gctggcctac cccgacgcgc aggacaacgc
     3661 ccgtctgacg ctgggcgatc tgttcgccca ccgcagcggc ctgcccgacc acgcgggcga
     3721 tcagcttgaa gacctgggtt atggacgcac cgaggtgctg gcgcgtctgc gccacgcccg
     3781 cctcaacccc tacggcagct acgcctacac caactttggc ctgaccgccg ccgccgaggc
     3841 gctggcccgg tccctgggca ccgactgggc cacgctgtcc gaacaggcct tgtaccagcc
     3901 actgggcatg acgcacacca gctcgcgcca cgccgacctg caagcgcgca gcaaccgcgc
     3961 ctggggccat gtgcaggcca atctgagcta cgacagctac ggcacccagc ccgcccgcta
     4021 tgtcgtgcag cagccccagc gccagccgga cgcccagtcg cccgccggcg gcgccagcgc
     4081 cagcgcggcc gacatggcgc gctggatggc gctggtgctg gccggggggc gctggcaggg
     4141 ccagcagttg cttgatccgg tcgcgctgca ggcggccctg gcggcacgcc ccggcggcac
     4201 ttacggctac ggcttcaacg tcgggcccga cccgcatggc caccccagcg tatcccattc
     4261 gggcgccttc ctgctggggg cgcacaccgc cttcatcctg tggcccgagg ccaagctggg
     4321 catcaccgtg ctcaccaacg cccagccgcg cggcctggcc gaggcgatcg ccctggcctt
     4381 tggcgaactg gccctgggcg acgcggcgga cggcgtgctg accgccgact ggctggccgt
     4441 gatgcagggc aggatgcgcg ggttgtacca gccgctgggt cagtacgccg gacaaccgcc
     4501 gcccgaaacg cccgcaccgc cccagcccct ggcccgctat gccgggcgct acaccaacgc
     4561 gtactacggc agcgcccagg tgacccccaa cgccgatggc aaaaccctgg accttgtgct
     4621 cgggccggca aaggtgctcc accggctgcg ccactggagc ggcgactcgt tcgtgttcga
     4681 gctgcaggga gaaaacgccc cgccgggctc cgtctcggcc gtccgtttcg cgccggacga
     4741 acgcgcgtac atgcagatcg agtactacgc cgaagacctg acacgcgggc gtttcgaccg
     4801 cgacgccgac accccctgac cgagcccaat cgaccgacaa cccaccacga ccgacaaccc
     4861 accacgaccg tcaaacaaca caaggaacct ctgaaatgcc catctcttct gcccactgct
     4921 gtcgcccaac ccccgtagca gccgcgctgg cgctggcgct cacccttgcc ctgactgcct
     4981 gcggcggcga cgacgacccc atttcgggca cgcccccacc gggcagtggt gatcccgcgg
     5041 ccaacaaatc catcgtctgc aaggactatg acaaccagga cgtgctggtc aagcccaaga
     5101 ccatcaccat ccgcaacaac tccgaaggcc ccatttaccc cgtgctggcc accagcatga
     5161 acctcaagaa cgagtgggtc atggcctgcc agcgcaccaa cgagctgatc gccaccgacg
     5221 cggtctacaa gctctacgtc aacgacggcc agggcatccc gcccggctct gaagtgacct
     5281 tgacgctgcc gctgtacagc gaactctcgc ccggcaaata cgtcacctgg tggaacggcg
     5341 ggcgcgtgct gctggccgac cgcaacaagc gcctgcgcgg cagcgtggac aagcccacca
     5401 acacgccccc cagcgtggac tgcaagggca caggcaccac ctgcaacctc agcacctaca
     5461 ccagcccggt gcagttccag gaggacgtgt ttgcgcagct gtccgaatac accttcggcg
     5521 actccatcat cccgcccggg cagagcactc ggctgctcaa gcccgagaac gtgggctaca
     5581 acatctccta cgtcgatcac gtctacatgc ccgtggccat tggcgtgcgg ggcaacccct
     5641 acatcggcta cagcggctcg gccaggaagc tgtccgactt ccgcgccacg ctgcgcagct
     5701 tcgtccagcc tggcagcctg ggcgaaggct ggccgctcta caacatgagc gagctgcgcc
     5761 tgcccggcgg ctacaacatc tttgcccagc gcggtggcta cctgctggaa gacccggacg
     5821 tgcccgtcaa gcccgccgac ggcaagaacc cgcccgtgct gacggtgctg aaatgcctgg
     5881 aaaaagacgt cgactgcgcc acgcccgagg cccagcgcaa catgcaatgg ggccaggcgg
     5941 tgcagaacat ccaggacctg tggggttcgt gcgtcgattg gggcagcgag gacatctcgc
     6001 agtacacccg caagaagtac ccgcaggact gccccgcgcc gcaggccatg cgcgaggagc
     6061 tggcgctcat caaagacttc ttcgcccaga accacaaaca gtacctggcg atgtacgcca
     6121 atcagcagtg cgtgggcgac cccgagggct cgcgcaagat tccggcgcac gtggccgagt
     6181 tcaagtactg ggaggccatc aagcacatct acggctgggt gcccttcaac gaaggctgcg
     6241 gcgcagatgc caacccgctg gccaagacca ccgtccacgg ccgcgaccat gcctacgtgc
     6301 agacgctcta catcgaagac ctgcaataca actacaggca ggccgccgtg caggccaacc
     6361 ccaaactggc cttcaacccc tacgtcaagc tgatccacga cgacctgggc atgagcgcct
     6421 atggcttctc ggtcgatgac gccgtgggct tcatgagcga gcagggcgac ggcctggtgt
     6481 tcaccgtcgg cggcaccgag gggttggaaa acgaccaggc gttcaactac gccgacggct
     6541 tctcgctggg cctgggcgtg ccgctggaga tcgacggcaa gctcaacatc cagatgatca
     6601 agaagtacgg cgtgtgctcg ctgggcgccg agccggccga ccccaattgc gagcagaaca
     6661 agcaggacat gatcatgccc gtgggccggc tggtcagcgg ctttcgcgtc ggcaccgtgg
     6721 cctcgtaccc tttgaaggtg cgcttcaccg acatggaaga caacgtctac accgtgttgg
     6781 tgcgcgagaa attcgccaag tgcaccggcc cgctgtcggc ctgccccagc aaccgcgcgc
     6841 agatcgtgga caaggccgcg tgcagcgtca tcaccccgca gggcaccaag cacccgcagt
     6901 ccgacacctg gtgcgccaac gccaacccca accaggcgcg cgacaacgaa caggcggtgg
     6961 tcaagaacca cctgagctac ccgaagccgg tgaagtggct gccgtgaccc tgcacggcgc
     7021 atcccccctc cgggggagat gcgtaaagac aaaaacgctt tcagccattg ataggcaatc
     7081 gatggcagct atcgattcag gagcataaga tgaaaagaac aagcattggc ctggccgccg
     7141 tggcggccct ggcactgccg ctggcagccc aggcggccac ccagaccttc accacacccg
     7201 gcagccactc gtggacggtg ccgcccggcg tcacccacat ccgcgtgcag gcgctgggcg
     7261 gtggtggtgg cggcggcgac ggcgtggcaa atgacggtca atccgcgtcg ggcggccccg
     7321 gcggtctggt gaccagcggc aacctttccg tcacgcccgg ccagaccctc accatcgtcg
     7381 tgggcggtgg cggcggcgcc agcggcgagc tggaaggcat gggtcaaggc ggcggcggcg
     7441 ggggagccag cagcgtacag gtgggcagca cctggatcgt cgccggcggc ggcggtggtg
     7501 gctcctacta caacgacaac agcgtctccg ataccggcgg ctggggccag ggcgnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccctgg ccgcgcacgc cgccatggac
     7681 gacgaagact tcgagcgcta cctgctgacc gctggcaagg gcacccccat cacgcccgag
     7741 accatcaagt cgctctctgt cggctccagc atcaactcgg cccagtccac ctacaacctg
     7801 cgccccatct tcgatctggg tcggctcaag gaagacggcc ggctgaacgt ggacaccacc
     7861 cgttctcagc aaaagaccgt gctgcgcgtc accagcaaca aagccgccag cacgtccgcc
     7921 gaacagctca acctcgatgt ttcggcctct ggctccgttg gccgcgaatc tttcggcgcc
     7981 aaggcttcag ccgccgttca ccatgccaac acccggcgcg acaccaagtc cgacggcact
     8041 gtcagcgtgc agatggtgtc ggccaacacc aacaacctcg tctccatcct gagcagcggc
     8101 ttttccggca gcgagaactt caccggctac ctgatcggca ccaagctgac cgacgagcag
     8161 ttgcgcggct acgttgccac taccgagagc acgcccaccg gcatctgcgg cggcaaaccc
     8221 tacaccacca gcgtcaaggt cagccgctac aagctgtccg acaccgaccc ttacgccaac
     8281 atccaaatcc tcacgcgcat ggagaacgta ttcgccgatc tgaaggcgca atacgaactg
     8341 tgcgccgatg catccatcca gaccgtgctg gtgcgccaga tgaccgagct gcgcggcaag
     8401 atcgaaagcg ccatcgccga cttctacgcc ttcaacggtg acgccttcgt ctcgcagacc
     8461 acctcgatga accaggccat cggcagcggc acgatgtcgt tcacgaccaa ggacggcaac
     8521 gctgaggagc agtacggcgc ctcggtatcg gcccaatacc agacgctgac cagcggggtc
     8581 ggcggcagtg tcaccgtgca gtactacaag cagcacggct gggccgaggc gctgctgaac
     8641 gtgcaggtct cggccgaggc caagcccgcc ggcgtggccg acaccacggc ctgggtcagc
     8701 agcatccaga ccatgctcaa ggaccagggc agctccctgg tgccgcccat gggcaacctg
     8761 cccaaggatc cgggcgtgaa gctgcccgaa ccggtgggcc tgcgccacga cgaggaagcc
     8821 ccgccggacg tggccttcac ctcctacgac cagtggaagc agtaccagca ggacaaaaag
     8881 gacaagaagg gcgtcaagga cgaggtcgtg ctggagcagg tgcagcagca aatcgaaaac
     8941 gagccgctgc gcatcaacga cgacaatccg caactgaaag ccacgcaggg cggcgacgcc
     9001 tacgaacagc tcatcgccga gctggacgcg ctcaaggagc gtgccacggc cccgcaggcc
     9061 ccaccgcagg gcaatgacgg caacctggtg cgcttcgaca agatgttcgt cagcggcttc
     9121 gaaacgctgc cctacgacgc cgtcatcccc cagctgcgcc ccgacctgga catacccggt
     9181 atcgaccagg gcatggggag cttccccaac atcatgaccg ccatgctgat gagcgaaaag
     9241 ctcggccggc tcgattcgta cctggccttt ctcgccaaca tctcggtcag caacgtcacg
     9301 cccgagatga gtgagcgcta ccaccagttc ttccagaagg cgtccgcacg cgcctacgac
     9361 ctggtgggcc tggccctgag ccagggcacc gacatgacgc ccgccgtgct ggccggctac
     9421 aagaaggcca tgctcggcac cgcatccgcc ccgacgcaaa gcgagctgta caagagcctg
     9481 caggacatcg actactacaa ctacgtggtg gacaccctgc tcgaccccgc ccggggcaag
     9541 gcgtggttcg tcgcgccggg cggctatatc cccatgcgct ggaagaacga cgaccgcggc
     9601 ggcgcggaac tggtcacctg gaccgggctg gcgccagcac ggggaatagc ccccggcaag
     9661 gaaagcgtgg tggccgtgga cttcaacaac cccaacgccg acccgctgac gctctaccgc
     9721 aacggcatga aaatcctgca gacgccctgg tacccggtct atgtcttcaa ccagggccag
     9781 gcgccggcgc tggtgttcgt gcagcacttc ggcgcctacc aggccatcta cggcgcccgg
     9841 tgggtgacgc ggccctacga cggcgacaac cccgtcaagc cgagcctggt gccccagtgg
     9901 caggactacc agccgctggg cacgcgggac atgcgcacgg tgatgacggg cgccaacaat
     9961 tcctttctgg ggcagttcat gaactgggac tactcggtgt acttcccgaa ttccagcaac
    10021 gaccctgtgc gcttgcgcaa gtacaacgcg ctcgtgctgc atctgcccgc cgattaccaa
    10081 gagagtgacg ggcagaccat gtacaccgcc gcgcactcgg cggcggtgcg cgatttcagc
    10141 catctcgctt acgacactca gtcgagaacc gacgccacct acaacgcctt ccgccagccc
    10201 acccatggcc gcaagatgcg gcgcatgagc gacggcgcgg tggtggacac cacgcactgg
    10261 acgctgggca acggcgcact cacctccgtg atgctgctgc cgctgaacac caccaccacg
    10321 agcgagagcc tgacgcaggg actgaactat gcgcctcagc gcgcgccgat ggacgttgtg
    10381 acccccacca gcctcgacct cgtcaacaaa ctggccgtgc tgctggagtg aaccgggcct
    10441 gccgcaggca acggcaggct gtttgaaaaa tcactttttg atagctgcta gcgcttatcc
    10501 agaaagcgct ggggccacaa acggtcagta gtcgtcgctg ctgcccatgg ccaggctctc
    10561 gaagcgcgtg agcggcttga tgaaggccag cttgacggtg ccggtggggc cgttgcgctg
    10621 tttgccgatg atgatctcgg ccacgccggg ctcgcgcgag tccttgttgt agtagtcgtc
    10681 gcggtagatg aacgtgatga tgtcggcgtc ctgctcgatg gcgccggact cgcgcaggtc
    10741 gctcatcatg gggcgcttgt cggtgcgctg ctcgacgctg cggttgagct gcgacagcgc
    10801 gatcaccggg cactgcagct ccttggccag ggctttcagg ccgcgcgaga tttcgcccag
    10861 ctcggtggcg cggttctcgt cgccgctgct gctgccgctc atcagctgca ggtagtcgac
    10921 cacgatcagg cccagcttgc cgcactggcg cgccaggcgc cgcgcgttgg cgcgcagttc
    10981 ggacggcgtc aggcccggtg tttcgtcaat atgcaggctg acgttgcgca gccgctcgat
    11041 ggcttcggtc aggcgcggcc attcctcgtc ggtcagcttg ccggtgcgca ggtggccctg
    11101 gtcgatgcgg ccgatggaac cgacgatgcg caccgccagc tgcgaagcgc ccatttccat
    11161 cgaaaacacc gccaccggca gctgctcgtg cagcgccaca tgctcggcga tgttgatggc
    11221 aaacgccgtc ttgcccatcg acgggcgggc cgccagcacc accagatcgc ccggctgcag
    11281 gccggcggtc atgcggtcga ggtcgatgaa gccggtcggc accccggtga cgtcgttcgg
    11341 gttgtcggcc atctcctgca cacggtcgag caactgaacc accaggctgt ccatggcctg
    11401 aaagccctgc ttcatgcgcg agctctgttc gccgatcttg aagatcttgc cttcggcgtc
    11461 gtccaggatc ttggccaccg gccggccctg ggtgttgaag gcgctggtgg cgatgtcgtc
    11521 gctggcactg accagctggc gcaggatcga gcgctcgcgc acgatctcgg cgtagcggcg
    11581 gatgttgctg gcgctgggca cgtactgcgc cagcgagttg aggtaggcca gaccacccac
    11641 ctcgtcgccc ttgccctggc gctgcagctc ttcgtgcacc gtgatcacgt cggccggctt
    11701 gctctcggcc accagcttgc cgatggcgcc atacaccaga cggtgctcga aacggtagaa
    11761 atcggcgtcg accagcacgt cgcccacccg atcccaggcg ccgttgtcca gcagcaggcc
    11821 gcccagcacg ctggattcgg cctcatggga atgcggcggc acgcgcaatt gggcgacctg
    11881 gcggtcggtg ggggcgtcgt cacccagatc ggcgtagccg gggggaaata cggaagacat
    11941 gcggcaggca gacagacaat ccgcccatgc tagcgcgcgc ggccggggct gtcactggac
    12001 aaggctgtgg acaaacggtg cagcgccggt gaacgccgca gccagagctg tggacatcgc
    12061 ggcaccgcaa aagacaaggg ccgcctgccg tgtctgcggc gggcggcccg ggctgggcct
    12121 ggagcggcgg cgctcaggcg gtttcgccgt acaccgagac cgtgatctcg gcgctcacgt
    12181 cggtgtgcag cgacaccgtg atggtgttgt cgcccacggt cttgatcggg ccggcaggca
    12241 ggcgaacctg cgatttgtgc acgtcgtagc cctgtttctt cagctcgtcg gcgatgtcgt
    12301 ggttggtgac cgagccgaac agacggccgt cgacgccggc cttctgggtc agcttgatgg
    12361 tctggccgtt gagcttctcg cccaccgcct gggcagcggc cagcttttcg gcggcctgct
    12421 tctcgagttc ggcgcgacgc gcctcgaact cggccttggc cgcctcggtg gcgcggcgcg
    12481 cacgcccgga ggggatcagg aagttgcggg catagccgtc cttgaccttg acgatttcac
    12541 ccaggttgcc caggttggtg accttgtcca gaagaatgat ttgcatggtt tgatcctcct
    12601 atcagacgcg gtgctggtcg gtgtagggca gcagggccag aaagcgcgcg cgcttgatgg
    12661 cggtgttgag ctgacgctga tagatggcgc gcgtgccggt caggcgcgcg ggcacgatct
    12721 tgccgttttc ggcgatgaag tcgcgcaggg tgtcgacgtc cttgtagtcg atctcttcca
    12781 cgcccgccac cgtgaagcgg cagaagcgct tgcgacggaa cagcagcgac tgggtgttgc
    12841 gcttgggacg cttgtccttg ttgaatttct tgaacgtggc catttgttag gctccttgaa
    12901 actgatcttg ctgaaaatcc tggatgtgga acaccaccga cctggtgcgc tgcgggctgg
    12961 cgagaaaacc gctgaagcag gcctgcgatc cgatgacctg ccggcccagc cgttcagcca
    13021 tcgcacccag agccaccgcc ctgagcgccg cccggacctg cctggactgg cctgcttcct
    13081 gctgggtcga ctcgtgctcg agccggagat ccagcgccgg caggccggca ggggtgtggc
    13141 gcagcgcacc gatttcgacg atgcgggcct ggaggcgcaa ctggttcacc gccctgcccc
    13201 gaggcaccgc gcacgccgcg tcagttggcc tccgcctgct gggccttgcg ggcctcctcg
    13261 cgctcgacgg tcttcatcat ggaagacgga gccgtctcgg ccttcttctt ctgcaccgtc
    13321 aggtggcgca gcacggcgtc attgaagcgg aaggcgtgtt ccagctcgcc cagcacggcc
    13381 tggtcggcct cgatgttgag gcacagatag tgggccttgg ccagcttgtt gatctggtag
    13441 gccagctgac gacggcccca gtcttcctcg cggtggatct tgccgccgcc ggcggtgatc
    13501 atgcccttgt agcgctccag catggccgga acctgctcgc tttgatccgg gtggatcagc
    13561 aaaatgattt catagtgacg catgaatgct ccttgtggat gaacgggccc gaggccgcca
    13621 gcggactcag gcaacaaccg cccccggcgt cagatcgggg tgcggcaagg caaccaataa
    13681 ttataaacgc aagtccggcc gcgcaccggc caggcgcgtg cgggcaccgt ctggcgtcac
    13741 ttcaggcgtg ccaggcggtc cttgcccgcc tgggccgcct ccgactgcgg gtaggtcttg
    13801 atcagcgact ccagcgtgcc gcgcgcggct cgtgtgtcct tcagctcgat ctggcagttg
    13861 gcgatcgcca gcatggcctc gggcgcccgc gcgtggctgg gcgccgcggt gagcagcgaa
    13921 cggaagttgg tgatggcttc cttgtagtcg cgcgtggcgt actgggcgtt gcccagccag
    13981 aacaaggccg acggcgccag tgtgctgcgc ggatagcgct tgatgaaatt ggcaaaaccg
    14041 gtctgcgcgc ctttgaagtc gcccgcacgg aacaccgcca gcgcggcgtc gtagtcctgt
    14101 ttttcactgg cggccgcacc gtcgctggcc acgggaggcg cccccgcggc ggcgttcgga
    14161 tcgctcatcc gtgcgctggc ctcctgctcc agcttgcgca ggcgctcgtc ccagccctgc
    14221 tgctgcgtcg actgcgcttt ctgcaggtcg gccagatcgc gtgccagctg ttcgtcctgg
    14281 ccgcgcgctc gggccagctc cgcgcgcagg gtttcgatct gctgctgcag atccagcatg
    14341 ccacggcgca gctgtgcgtt ttcgtccacc agccggttct gcgccgccac cgcctggtcg
    14401 aagcgctggc gcagatcgag gatggcctgg cgcgcctggt cgtcgccgaa cagctgggcc
    14461 tgcgccccct gcccggccag acaggccagc gccagcaccg cggcacgcag gctcgtcacc
    14521 ctgaagatcg ccatcgagcg ctcagcggta gcggatttcg acacggcggt tcttggcata
    14581 ggcatcttcg ctggcgccag cggctgccgg cttctcctcg ccgaaactga ccgcttccac
    14641 ctgcgcgtcg ctggccccca cgagcgtcag ggcacggcgc acggcctcgg cacgcttctg
    14701 gcccagcgcc aggttgtatt cccggctgcc gcgctcgtcg gtgtggcctt ccagcgccac
    14761 gctgcggcca cggttggcct gcaggaagcg cgcgtgctgg tcgaccagcg actggtactc
    14821 ggacttgacg acaaagctgt cgaaatcgaa gtagatcaca cggcccacgc cgttcgggcc
    14881 tgcggcatca gcgccggccg cacccgcctg cacctgggtc accgtgctct ggccggcacc
    14941 ctggccgctg gcaccgccct gtccggcagc gctggcgcca gcgctggcgc tgccctcgat
    15001 cggcggatcc agcttgacgt tggagccgca accggccagc acggccgcca gggcagccac
    15061 catcagatat cgcttgaaca tgaaatcact cctgaactga acacgacaat tcgatgcacg
    15121 acccacgcgg ccatcgctca tcagcgctgg tacggtcccc aggccggttc gcggatgtca
    15181 ccgccctggc ccgccagacg ggccttgatc ttgccgtcca gcgtggtggt catgagggcc
    15241 tcgcggccgc cctggcgcgt ggcgtagacg atcaggcgac tgttgggcgc aaagctgggg
    15301 ctctcgtcct cggcggtgtc ggtgaccgcg gtggtggtgc cgctggccaa ctccatcacg
    15361 tggagcctgt agccattggt gcgcgacaca taggccaggt agcgaccgtc gggactgatg
    15421 gcgggcgaga cgttgtagct gccgctgaag gtgacgcgct gcgcggcgcc gccgcccacc
    15481 ggcatgcggt agatctgcgg cgcgccgcca cggtcgctga cgaagtagat gctggcaccg
    15541 tcgggcgaga acgcgggctc ggtgtcgatg ctttgcgact gcgtcaggcg gcggggctcg
    15601 ccgccgctgg cgctgatcag gtacagctgc gagccaccgt cgcgcgacag cgtgaccgcc
    15661 agactgcggc cgtcgggcga ccaggccggc gcgctgttgg agcccttgaa gttggccagc
    15721 aggcgccggc ggccgctggc cacgtcgtgc acgtagatga ccggtttgcg cgactcgaac
    15781 gacacgtacg caagctgccc gccgtcgggc gaccagacgg gcgagatgat cggctcgggg
    15841 ctgcccagcg cggcctgggc gttttcgccg tcggagtcgg ccacccacag agtgtagcgg
    15901 ctgccgccct tggtgacgta ggccagtcgg gtggaaaaca cacccttgtc accggtcagc
    15961 ttctcgtaga tgtagtcggc gatcttgtgc gccgccagcc gcagatcggc ctgcggcacg
    16021 acgtagccca gtccaccgag gtcctgcccg cgcaccacgt cccacaggcg aaagcgcacg
    16081 tcccaacggc cgtcggccag gcgggtcaca ctgccaccgg ccagcgcgtc ggccgtgcgc
    16141 tggcgccaca gcgacatctc gggccgggtg gtttcgtcca tggactggcc gccggcatcg
    16201 acggcgcgga actggccact gcgctccagg tcggcgcgca cgatggcgct gatcttctgc
    16261 ggcgccgcgt cttcgccgcg aaacgggacc agggcaatgg gcaactgggt caggccgaca
    16321 cccgaaacct caacccgaaa ctgtgccagc gccgccgaga acggcgccgc cgccagtgcc
    16381 gtgacgaacc cgcggcgttg ccatggggca agggaaaact tgtcgagaag agcagatttc
    16441 ataggcacag gccgcagcgc catcacatcc gagcaatgca catcggcgcc ggcagatcgc
    16501 caaagccggg caatggtaac aagcccaacc ggggcggcgt ttcagaatgt gatttggtgc
    16561 cagcgacgtg tgggaagttc cacgcatttt ccaccgcggc gtgaccgccg caggggctgt
    16621 cgtggggcct tcgtagaatc cacgcacccc gcaccccgcc catggccgcc ccacccgctc
    16681 ccgccccgcc cgcgtcaccg tctgcgcagc agcccctggg ccggcgactg gcacggctgt
    16741 ggacctactt ccgcgaatac cgcctgggct ggctgatcgc cgccctgggc accctggtga
    16801 gcgcgctgac ggaagcctcg gtgccggccc tcctcaagcc gctgctggac gagggcttca
    16861 cccagggcag cctgccgatc tgggtggtgc ccctggccat cgtcgggctg ttcgccctgc
    16921 gcggttttgc ccacttcgcc agccagtacg cactggcgcg catcgccaac gacggcatgg
    16981 tcaagctgcg ccggcagttg ttcgaccgcc tgctggtggc cgagctgtcg ctgttcgcgc
    17041 gccagagcgc cagccagctc gccaacacgc tggtctacga agtgcagacc ggcaccacct
    17101 tgctggtgca ggcgctgctg aacctggcgc gtgacagttt ctccgtcgtc gcgctgctgg
    17161 cctacctgct gtacctgaac tggtcgctga cgttgatcgt gctggtgctg gtgccgatcg
    17221 tggcgctgat catgaagacc atgtcgcgcc gcttgtaccg ctacacccgg ctgtcgcaga
    17281 ccaccaccga cgagctggcc tatgtggtgg aagaaaacgt gctggcccac cgcatggtgc
    17341 gtctgcacgg cgcgcagggg cagcaggccg gccgcttcga caccctcagc cgccagctgc
    17401 gcgggctgtc gatcaaggcc accagcgcct cggccgccgc catgccgctg gcgcagatgg
    17461 cggcggcggt ggcgctgtcg gtggtgatcg gcattgccct gcaccagggc cagagcggcg
    17521 ccgggcagcg catcacggtg ggcggtttcg tgtccttcat cggcgccatg ctgatgctga
    17581 tccagccgct gcgccgcctg accgacgtgg tcgggccggt cacgcgcgga ctggcggcgc
    17641 tggagcgtgg cctggacctg atggcccagg tgcaacccga agccggcccc gccgccaccg
    17701 caacgagcgc ggcgtcgcgc gcccttggcg agctgtcact gcgcgaggtc acggtgacgt
    17761 accacggtgc ggccgagccg gtgctgcagc agctcagcct gcacgtgcgg gccggcgaga
    17821 tggtggccct ggtggggccg tcgggcgccg gcaagacgac gctgatcaac ctgctgccgc
    17881 gcttcgtgcg accgagctcg gggcaggtgc tgctcgatgg ccaggatatc gccgactggc
    17941 ctttggcggc gctgcgggcg cagtttgccc tggtcagcca ggacgtgacg atgttcaacg
    18001 acagcatcgc cgccaacgtg gcgctgggcg cgccgtcgat cgaccgcgag cgtgtgcagc
    18061 gctgcctgca ggacgccaac ctggccgacc acgtggccac gctgcccagg ggcatcgaca
    18121 ccgtgctggg ccacaacgcc accgaactgt cgggcggcca gcgccagcgg ctggccatcg
    18181 cgcgcgcgct gtaccgcgac gcgccggtgc tgctgctgga cgaagccacc tcggcgctcg
    18241 acaccgaaag cgaacggctg gtgcagcagg ccatcacacg cgccatggcc gggcgcacca
    18301 cgctggtcat cgcgcaccgg ctgtccacca tcgaacacgc cgaccgcatc gtggtgatgg
    18361 agcgcggccg catcgtcgag caaggcagcc acgccgagct gacggccgct ggcgggctgt
    18421 atgcgcggct gcacgggcag ccgtcggcgt cggacgccgg cagcccgggt aactcctgat
    18481 ttcatagctg ctggcgcgct ccagataagc gccggcaccc catttcgttc atcaccgcac
    18541 gggctgaacc ggcgtcggcg gcggcaggtt ccgtgtgcga ggggctggcg cacagacccc
    18601 acagacccta cgatggcccc tgcgccaccg cgcaccgatg gcaagcgttc gtgcgccgtg
    18661 atccggcgcc ctgtcttgtc cgacgacaaa cgcacgacac gatcgtgccg atggagaccg
    18721 agcatgtcac cacactcagc cctcaggctg catgtgcccg agccgacggg acggccgggc
    18781 gccaagaccg atttttccta cctcagcatc tcaccggccg gtgcggtgcg ccaacccccg
    18841 atcgacaccg ccgcggccga caccgccgac ctggccacca gcctggtgcg cgtgctcgac
    18901 agcgagggcc gcgccgtcgg tccctgggcg ccagccattc accccgagcg cctgcgccgc
    18961 ggcctgcggg ccatgctgaa gacgcgcctg ttcgacgccc gcatgttgct ggcgcagcgc
    19021 cagaaaaagc tgtcgttcta catgcagtgt ctgggcgagg aagccatcgc cgtggcccat
    19081 gcacaggcgc tgcgcgaagg cgacatgtgc tttcccacct accgccagca gggcctgctg
    19141 ctgtcgcgcg acgacatctc gctggtggag ctgatctgcc agctgatgag caacgagcgc
    19201 gacccgatca aggggcgcca gctgccggtg atgtattcct acaagcgcgc cggcttcttt
    19261 tcgatctccg gcaacctggc cacgcagatg ccgcaggccg tgggctgggc catggcctcg
    19321 gcgatcaagg gcgacaccaa gattgcctcg gcctggatcg gcgacggctc caccgccgag
    19381 agcgactttc acacctcgct caccttcgcc catgtgtacc gcgcgccggt gatcatcaac
    19441 gtggtcaaca accagtgggc catctccacc ttccagtcga ttgccggcgg cgagggcacc
    19501 accttcgccc agcgcggcgt gggtgtgggc atcgcctcgc tgcgcgtcga cggcaacgac
    19561 ttcctggccg tgtacgcggc ctcgcaatgg gcggccgagc gtgcgcgcgc caaccatggc
    19621 ccgacgctga tcgagtggga aacctaccgc gccggcccgc attccacctc cgacgacccg
    19681 tccaagtacc gcccggccga cgactggcag cgctttccgc tgggtgatcc gatcgaacgc
    19741 ctgaagcagc acctgatcgc catcggcgaa tggagccagg cccagcacga agccacccac
    19801 aaggcgctgg aagccgagat cctggcggca cagaaggagg ccgaaagcca cggcagcctg
    19861 ctcgacggcc gcgtgcccag cgccgcgacc atcttcgacg acgtgtaccg ggacctgccc
    19921 gagcacctgc gccggcagcg ccagcagatg ggggtatgag ccatggagca agctgtccag
    19981 cacgccaccg ccacccccat gaccatgatc gccgcgctgc gctcggcaat ggacgtgatg
    20041 atggcccgcg acgacaacgt catcgtctat ggcgaggacg tggggtactt cggcggcgtg
    20101 ttccgcgtca ccgaaggcct gcaggccaag tacggcaaga cgcgctgctt cgacgcgccg
    20161 atctccgaat ccggcatcgt cggcaccggc atcggcatgg ccgcctacgg cctgcgcccg
    20221 gtggtggaga tccagttcgc cgattacgtc tacccggcca ccgaccagat cgtgtcggag
    20281 ctggcgcggc tgcgccaccg ctcggccggc gacttcagcg cgccgatggt gatccgcatg
    20341 ccctgcggcg gcggcatcta cggcggacag acgcacagcc agagccccga ggcctttttc
    20401 acccacgtct gcggcctgcg caccgtcatg cccagcaacc cgtacgacgc caagggcctg
    20461 ctgatctcgg ccatcgaaag cgacgacccg gtgatcttcc tggagcccaa gcgcctgtac
    20521 aacggcccct tcgacggcca ccacgaccgg ccggtggtgc cctggtccaa gcatgagctg
    20581 ggcaaggtgc ccgaaggcca ctaccgcgtg ccgctcacca gcgccgccat cttccgcccc
    20641 ggcaaggcgg tgacggtgct gacctacggc accatggtct gggtcagtga gtgcgccgcg
    20701 cgcgaaaccg gcatcgacgc cgagatcatc gacctgcgct cgatctggcc gctggacctg
    20761 gacaccatcg tcgaatcggt ccgcaagacc ggccgctgcg tggtggtgca tgaggccacg
    20821 cgcaccagcg gcttcggcgc cgaactggtg gcactggtgc aggagcactg cttctggcaa
    20881 ctggaagccc cgatcgaacg cgtcaccggc tgggacacgc cctacccgca cgcgcaggaa
    20941 tgggcgtact tccctggtcc gggccgcgtc ggcgaggccc tcaagcgcac gctggaggtc
    21001 tgaaccatgg gtacctacgt gatccgcgtg cccgacatcg gcgagggcat tgccgaggtg
    21061 gaactggtgg cctggcatgt gcagccgggc gatgccgtga gcgaagacca gcacctggcc
    21121 gacgtgatga ccgacaaggc cacggtggaa gtgccctccc cggtggcggg caaggtgctg
    21181 acgctgggcg gtggcgtcgg ccaggtcatg gcggtcggcg ccgagctggt gcgcctggac
    21241 gtcgaaggcg ctggcaactt caaagaaaat gaggcttcag cgcccgcccc atctgcgccg
    21301 gcagctcctg tttcaatagc aaccgaggtg atcgcccaag ccccggacgc agcaccgcgc
    21361 gcacccgcgg ccgccgcagc gcgcggctcg gtggccgcgc cgcgccctgt cgccggccgc
    21421 cgcaaggagg gcgagcgccc gctcgcctcg cctgcggtgc gccggcgtgc gctcgacctg
    21481 ggggtggagc tgcgcttcgt gcacggcagc ggcccggccg gtcgcatcgg gcaccaggac
    21541 ctggacgctt acgtcgccgg acagggtcag gccatgctga cgggggcgtc gccctacgcc
    21601 gagcgccatg gggaagaagc cgtccccgtc atcggtctgc gccgcaagat cgcgcagaag
    21661 atgcaggagg ccaagcgccg catcccgcac ttcagctatg tcgaggaaat cgacgtcacg
    21721 gaactggagg cgctgcgcca gcagctcaac cagcgccacg gcgccacccg cggcaagctg
    21781 accctgctgc cgtttctggc gcgcgccatg gtgtgcagct tgcgcgactt cccgcagatc
    21841 aacgcccgct acgacgacga cgccggcgtg gtcacgcgcc atgcgccggt gcatctgggc
    21901 gtggccacgc agaccgatgg cggcctgatg gtgccggtgc tgcgccatgc cgagagcctg
    21961 gacctgtggg cctttgccgc cggcctgacc cgcgtcaccg aggcggcccg caccggcaaa
    22021 gccgcgcgcg aggagctgag cggctcgacc atcaccatca ccagcctggg gccgctgggc
    22081 ggcgtggtca ccacgcccgt catcaaccac cccgaggtgg ccatcgtcgg tgtcaaccgc
    22141 atcatcgagc gacccatgct gcgtggcggt caggtggtgg cgcgccagct gatgaacctg
    22201 tcgtcctcgt tcgaccaccg cgtggtcgac ggcatggacg cggcgcgctt catccaggcc
    22261 atgcgcgccc tgctcgaaac cccggccctg ctgttcgtgg agtaggcacc ccaaatgacc
    22321 aaggaagtca cgaccaagct gctcatcatc ggcggcggcc caggcggcta cgtcgccggc
    22381 atacgcgccg gccagctggg catccccacc atgctggtcg aaggcgccag cctgggcggc
    22441 acgtgtctca acatcggctg catcccgtcc aaggcgctga tccacgcggc ccacgagttc
    22501 gaccaggcgc gcgcgcaggc aaccggctcg cccaccggca tccgggtgca gcgcccgcag
    22561 atcgacatcg cgcagaccgt gcgctggaag gacggcatcg tgcagcgcct gacgggcggg
    22621 gtcggcggcc tgctgcgcag ggccggcgtg caggtgctca agggctgggc cgagatcgaa
    22681 gacggcaaga ccgtgaccgt caaaccgcac cagggcgagc ccatgcgcgt gcgctgcgag
    22741 cacctgctgc tggccaccgg ctccgaaccg gtggagctgc ccagcatgcc gttcggcggc
    22801 gccatctggt catccaccga cgcgctctcg cccgatctgc tgccccggcg cctggtggtg
    22861 gtcggcgccg gctacatcgg cctggagctg ggcatggcct accgcaagct cggcgtggac
    22921 gtgacggtgg tcgaagccgc ccagcgcgtg ctgcccagct acgacgagga actgaccgag
    22981 ccggtgctgc aagcgctgca gaagcagggc gtggtgctgc acctgggatg cagcgtggcc
    23041 ggctgggacg cccagcacgg tgtgcacgtg cgcatcgacg cccgcgccga ggactttgcg
    23101 ctgccggccg accgcgtgct ggtggccgtg ggtcggcgcc cgcgcaccgc gggctttggc
    23161 ctggagtcgc tgcagctgga catggccggg cgccacatcg ccatcgacgc gcacggccgc
    23221 acctcgatgc gcaacgtctg ggccatcggc gacgtcaccg gcgagcccat gctggcgcac
    23281 cgcgccatgg cccagggcga atgcgtggcc gagcagatcg ccggcaggaa ccggcgcttc
    23341 gagcccatgg ccattcccgc cgtgtgcttc accgaccccg aggtggtggt gacgggcaag
    23401 acgcccggcg aggcccgcgc cgccggcatg gactgcatcg acgccgcctt cccgttcgcc
    23461 gccaacggcc gcgccatgac gctggaggct gccagcggct tcgtgcgcgt ggtggcgcgc
    23521 cgcgacaacc acctgatcct gggctggcag gccgtgggca agggcgtggc cgaactggcc
    23581 gccgccttca gccagtcgat cgagatgggc gcgcggctgg aagacgtagg ccacaccatc
    23641 cacgcccacc cgacgctggg cgaagcggtg caggaagcgg cgctgcgcgc gctggggcag
    23701 gcgctgcaca tctgaggcgg gcgcttggcc gcgcccgtcc ggttacaagc caaatcgggc
    23761 gccagcgctt gtcagtgaag cgccaacagc tattctattg gtagcatttt cagctgaacc
    23821 ctgtgctcgc cccgccaccg gctcgaccct ctgctcaatc cgcaggcagc gcccgccagc
    23881 ccaataccgg cgcggcttgc gcggcttgtt cagggggttt tccacagccc ggcccacagt
    23941 tggggtgcat aagtgcggca agccacgcca gtgcaacgcc cctgccgcgt ggcgttggca
    24001 tgacgaatga cttcgccgct gcgcggcatc aatgacaaaa ggggatggcg ccgttgacat
    24061 tggtcatgcg aaccgcagcg agctcattcg tcagggctgg cgatcagcgc gctgcgccgc
    24121 gcgccaggcg gcgggcgtgc tgccactcca gcgcttgaag gcgcgccaga acaccgctgg
    24181 ctcggcaaag cccagggcct ggccgatgtc ggccagcggg cgcgtggtct gcccaacggc
    24241 ctgcagcgcc gcgtcgcgcc gcacgccgtc cagcagctcg gcaaagctga tgccctgcgc
    24301 ccgcaggcgg cgcgccaggg tgcgttcgct cacgcccagc gcggcggcgg cctcggcccg
    24361 cgtgggcacg cggctggcca gcgccgcagc catccaggcg cgcagctcct gcgccagcgc
    24421 cccgccctgg ctcaggccgg ccagatgcgc cgccgcttcg cgctggtgcg cgcgggcgcg
    24481 gtgcgggtcg gcatgcggca gcggcgcgtc cagcacggcg ttgtccagca gcaggccgtt
    24541 gaaggcctgc tcgaacgcca gcgggcaggc aaacacctcg cgatagccgg ccagcggccc
    24601 cacgcgcggc tggctgaact gcacctgcag cggccgcaca cccgcgcccg ggcgctggcc
    24661 cagtgcattg cgcacaaagg ccagcacggt ggccagcacc gcctccacct ggtgcgggct
    24721 gaaggccagc gtgccctggc gcgggtggta gatcagccag ctggcgcgcg cgcccgccac
    24781 catctgaaag cgcccgccgt cgctgatcag gcgctggtac ttctgcaccg ccgcaatcgc
    24841 ctcgcgcagc gtggcgctgg cgcgcagcag ctcgcccacc acgttgaagt tatccggccc
    24901 ggcccggctg ccggcggcca ggccaaagcc cgcgtcctgc gtgcaggcca ccgccgcgtg
    24961 ccacaggcgg gtgatgtggt cgatcggcca gcgttcttgc tgaagttcct cgggtgcgat
    25021 accggcctgc gccagcaagc gcccacgccc cacgccctgg cgctcggcct cggccagcac
    25081 cgcatcgacc cagctcatgg agaccgtggc ctgcaaggtc atggcccggc cctcaacgcg
    25141 gcggcgccac ctgctcgccc cgcatgcgcc gacccagcca ggccagcgcc ggcccgaccg
    25201 gcacggcgcc acccaccgtc tgcgccaccg gcagcggcgc aaagggcgat ccgcgctgcg
    25261 ccaccgcaaa ggcgaagctg tagtgcggct ccagccccat gcgcaggtca gtgatcacca
    25321 gctggccgcc cgcgctctcg tgcagcttgt aaaagccgtg gctgaagcgc cggatgcgct
    25381 gcgcgccgtc gatggccgcc accgccgctt ccaggcgcgc gccgcggtca aagcgctcaa
    25441 agcgcatggg cccgggccca tcgagcaggc catgaaagcc ctcgtagtag ccatcgccct
    25501 ccatagccac cacgcgccac agcagcatgg acagcggcgc gggcgtgacc agcacgcgct
    25561 caggatgaac gccctgcgcg gccaggctgg cgcgcgcaag atgctccacc cgcgcctgca
    25621 gccccacacc ggccagcagc accgccgtgc ccagcgccag cccccacaga ttggcgcgct
    25681 ggccgcgcac atcgccccgg cgcgccagca ccaccaccag gccgacgagc cacggcagcg
    25741 tcacggccgg gtcgatgatg aacacgctgc ccagcgccac cgggtagtcg gtcaacggca
    25801 gcagcaactg cgtgccatag accgtgaacg cgtcgagcag agggtgggtg accagggcca
    25861 gccacagcgc cagccaccag cgccgccact gcccccattc gccctgcaca cgcgccaccc
    25921 cccaggcgaa cggcagcgag aacagcgtga gccagaacag cgcatggctc tcggcgcggt
    25981 gcagcaccat gttgaggatc gggtcgccgt gggcgatgag cacgtccaga tcgggcagcg
    26041 tgccggccac gccgccccac agcgcggcct tccacgcggc cgtgcgccgg cgcatcacgg
    26101 ccacgccgac ggcggcgccg aggatgagtt gggttgcgga atccatggga agaattgtgc
    26161 gctgccagcc cgcgctcaaa gatgagactg gacatactca cggctgctgc gcaggaacgg
    26221 ggaaatttgt atggatgacc tgaagggaca ggggacatcg ttgccagcgg tgcgacgtga
    26281 tgtgtcgcac aaggcagtaa gagcagccac agactgcgga cattgggctg ccccaggatt
    26341 cgcccgatcc agcgattacc gccctcttcc acgatgaggc aaaccctctt ggaccggttt
    26401 tcgcagcatg acgccttgcg aagaaggctt gaggcggtgg accgcccaat tgcagtgcaa
    26461 ggaattcccg agggcctgct gggcgaagtg ctttgcgaat tgagtcgata gctatgtcgg
    26521 gttgcaatgc ttctcgatca gttggcaaaa agctcggaca gattgacttc gcctgccttc
    26581 gcggcgatca gtcggacctt ctttgccttc agagcgtcaa agccgtttac cttgattttg
    26641 ttgtactggt tggcagctgt accaaacacc ttctccatcc gtgccaagaa gtgtgtggat
    26701 gtcgcttctg gaaccacgat gtcgatccga cggtcggggt ggcaccgaag gatgtcgttg
    26761 aagtgctcgt cagcgttgtt gaaggagtac cccacgacga tgacatgttc agcctgcttg
    26821 acccattcgg acgcttggct ccatagctcg atgtaccggt gggaaagaat cggcttcaac
    26881 cgaagcggtg gcaccagggc gggaatcaca tgccgttgct gtcgaaggtc atcggcactg
    26941 acatccacgt tcggggtcac gacttctcga atgaacttcg cagggtcgca attgaggatg
    27001 tcatcgaccg gaagcaggtc gcgtgtatcc atgcggacgt aatcgctcaa tccaccatgg
    27061 aagtagatgg tttttgcggc gccgagactt tgctcaagga aggcggtgta gttcagcgtg
    27121 atcgcgcgga ggtcgtcggg caccttgccg tagaaaggca acgggcttgc ggcatgcgct
    27181 tcgagaactt ccttctgctt cgcaacgagg taggcccaca gtgcccaggc gatgtacagg
    27241 tagtttttga tgtcggcagg cttgtcgttg tagaagccca agaatttttc gatcagcagc
    27301 gtttcgatgt tgagcatgtc cgcgcccagc gcagccgcga cctcgtggcg ctcggagctg
    27361 agccccagct tcaaggttct tttcaacacc gtcttgaagg tgtcggacag cgagagcttg
    27421 tgaatgtcga ggatcgaatc gctgtcgatc agttcgtcgg catccttcgg gaacacctcg
    27481 cgaatcaagg cctcggtgtc ggcatcaagt tggctctctt cagcgatggt ggccagcttg
    27541 ttgaacaggc ggatgatgag ttcgccatgc ttacgaatgc cttcttggtc ttccccaaga
    27601 tgcgcgatgg cgtcttgcac cctttgaacc atagccttct gctcgtgggg ctccctggtg
    27661 gcgatcttgt cgacagcgcg ggcgatcatg ctgttgaagc tgaatcgcag tccggggagc
    27721 atgtcgcgca gcgccgtctc gacgtcctta ccagggccat cgagaaaacg agtcacgtcg
    27781 gccaacaagg tgttggccag tggaaagtca atgccggccg ttcggtcaac gccagcgccc
    27841 aagatcagaa ggtgttttgt cattcttttt ccatgaagtt tgatgttcta gttacttcac
    27901 caactccatc aactaaccga acacccttag tcaccgcgcc cgccttcctg gcgccggatg
    27961 ccacgtggtg gggtgatttc tacatatgcg gttgagagca gacaggcgtg cggtacggcc
    28021 ttgcgtgcga ggaacctctt tatacttggt ttgaaacatt cagcatcata agtttcgtta
    28081 ttccaaggtt tgtagaaacc ccacattgga ggatggagca tggatccttt cgggatcgtc
    28141 gctctggtgg ttgccggtgg ttggctcatc ggcaagctca acgacgacaa caagccgaag
    28201 ccacccccgc cgagtgggcg ggccccgcca tcacctactt catcaccagg agcgcggggt
    28261 gtctatcacc ccgtcgcccc ggatcgggtt caccggcctg ccaaacaggc acaggccgaa
    28321 gacgccatcg aagtcctgcc cgaatacaag ctggtgcgtg atctggtgcg cgccggcttc
    28381 ccgatcacct ttgtcacagg tggtgcgggc accggcaaaa gcaccttcgt gcactggctg
    28441 acctcacagt tcaacggcac ggtgctcctg acggcgccga ccggcattgc cgctctcaac
    28501 atcaacggca agacactgca ctcgctgtgc aaactgccac ccgcctggat caccaagcgg
    28561 gacatccgct acgcggggcg cgacatcgtc cagcacgccc aactcttgat cattgacgag
    28621 atttcgatgg tcaacgcaaa tctgctggac gccgtcagcg ccttcttctg caagaaccgc
    28681 gactccgacg cccccttcgg cggcctgccc gtcgtgctgg tcggtgactt gttccaattg
    28741 ccgcccgtgg tcaacggtca gacacggcac ctcttcgaaa agcactaccc cacatccaaa
    28801 ttctttggcg cgcggctgtt gcgcgatgcc ccctactacg cactggagct gcacaaagcc
    28861 ttccgccagg cggatcaaac atttgtcgac ttgctggcca agctgcgcga aggcgtagac
    28921 gtggccgaca gcattgccga actcaatcga ctctgcacca ttacagaccg accgcccgac
    28981 caagccgtct ggctgtctcc acgcaacaag gaggtggacg cccgcaactg ggccgagctg
    29041 cgcaggctgc ccgccccatc gcgcttctac caaggcaaac tgaccggccg gttcagggat
    29101 gaccgcctgc catcgcccaa gaggctggag ctgcgcatcg gcgcgcaggt catgtttacc
    29161 aagaacggcg ggcgttgggt caacggcacc attggcgtcg tggttggctt gctggacggc
    29221 cgcgtggaag tgcggctgca cgaaaccggc accgtcgtgg acgttgggcc ggtgacatgg
    29281 gagcagtttg actaccacct caaccagcaa accaacgaca tcgagcgcac cgtggtgggc
    29341 gaataccagc agataccgct gatgctggcc tggtcggtca ccatccacaa aagccagggc
    29401 aaaaccattg accgggtgca catcgacctg ggggcaggcg cattcgagac cgggcagacc
    29461 tatgtggcgc tgagccgatg ccgctcgctc agcgccctga ccctgtcgcg ccccctgcgc
    29521 gaatcggacg tgctggtcga tgccgaatcc aggcagttct acggcaagct gcgcgagctg
    29581 atccgcacgg ttccgccgca ggccatggcg cagaagatgg gggtggcgtg gggtaacaca
    29641 cctgcgccag cgccggcgtc acgcaagtgc ctgccgctgg ccaacaaagc atagccagcc
    29701 gaccgctggc gcaaaaacac agacggtcgg cgcaaagcgg acggtgtggt agcagccccc
    29761 ctggccgcac cggtacagca gcgcggctgg gaatatgtgg tgctggcttc cggccggttg
    29821 cgctgacccg cgcacggcac agcgccccgg gattgacctc caaagctaat ttctctgtcc
    29881 tccaaagtca atcacaacca cccccgcgcc gctatctttg cccccaggag acgactggaa
    29941 tggcaacaac agcatccgac accctggtca tcggcggcgg cctggcgggc atcgtcacgg
    30001 ctttggaact gctgcgcgcg gg
//
LOCUS       DAOOOG010000013        26711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_1637_length_26711_cov_28.433111, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000013 DAOOOG010000000
VERSION     DAOOOG010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 26711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26711
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1637_length_26711_cov_28.433111"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="PK242_02205"
     CDS             complement(<1..112)
                     /locus_tag="PK242_02205"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HOZ92866.1"
                     /translation="MSAATLVEIDVRNIPPYQRHQQIFAQVDALQVGQALQ"
     gene            complement(199..1410)
                     /locus_tag="PK242_02210"
     CDS             complement(199..1410)
                     /locus_tag="PK242_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HOZ92867.1"
                     /translation="MTQRSAPLMSIDDRAQPPAALPGWPLLRLGFRPFYLGTALLACV
                     SIPLWIAAFLGHMPLRLPFPILLWHAHEMLFGFAAGVVIGFLLTAVRAWTGLETPRGP
                     VLGLLALLWLAARLAAVLAPYPLFAVLDLLLLPAVAFFLLRVLIRAGNRRNIPLISLL
                     LAMALANAVFHLSVLGLIAVPAMSALYAELALILMVVSIMTGRVVPMFTKNVTPGLVI
                     QVSRRFELTLLAVTAVTLALWVLGAPAWLVLPASALSAALHALRLWKWHPRVTLRRPI
                     LWILHFSYAWMPVGFALLALAQPGWVAPSLAVHAFGVGVIGGLIIGMITRTARGHTGR
                     PLQASRGEVVAYALVMLATVLRVLVPAVVPAWYVRALDSAAGLWALAFAIYLVIYVPW
                     LTRTRLDGKDG"
     gene            complement(1533..2228)
                     /locus_tag="PK242_02215"
     CDS             complement(1533..2228)
                     /locus_tag="PK242_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOZ92868.1"
                     /translation="MLKLYIGNKNYSSWSMRPWVLMKQAGIAFEEVQLRFDSFSGDSQ
                     FKQRLGRISPAGKVPVLTDGELVVWDTLAIAEYLAEKFPELHLWPRSAPERARARSVC
                     AEMHSGFGALRSACGMNIEAQLPEAGAIIWRDRPDVRADVARIVEMWQELLAMHGGPM
                     LFGRFGIADAFYAPVVMRLKTYALPVPDDVAAYMARLCALPGVAAWIEGALAEQDFLP
                     FEEPYRAARPASA"
     gene            complement(2250..3218)
                     /locus_tag="PK242_02220"
     CDS             complement(2250..3218)
                     /locus_tag="PK242_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HOZ92869.1"
                     /translation="MGKRILVLGGSGFVGRHLIEHLQRLGVRVTVPTRRRDAAPWLWS
                     LPRVDVVPADVTDPAHLGALLAGHQAVVNLIAVLHGDARRFEQVHVELPRRIAAACAA
                     SGVRRLVHISALGAAADGPSRYQRSKAQGEAVLQQAAVDGALALTLLRPSVIYGSGDH
                     LLNLFAGLQRLFPLIPLASAEARFQPVWVRDVAQALVRCLQDDTTAGRTYELCGPEVW
                     TLRELVRAAGMWAGVRGGRGRPIVGLPAALGRLQALAMECLPGEPLMSRDNLDSMRVD
                     NVASGQLPGLAELGITPAPLSAVGPMIVGADHPAARLDAYRRSAGR"
     gene            complement(3295..5235)
                     /locus_tag="PK242_02225"
     CDS             complement(3295..5235)
                     /locus_tag="PK242_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOZ92870.1"
                     /translation="MLALPADAAARDAVLLDMRDAHRRGDKARLTALLPQARGHALEP
                     WAAYWELKARLEDAGATEVQDFLARYAGSYQEDRLRNDWLLLLGKRRDWAAFRAEYPR
                     YRMRDDRELRCHAVSADLAAGLAPGPQAVEQVRRDWLAQRDADDGCLYAAGLLHQTGL
                     LPYQVLWKKARLAMEANRPALARAAVALDAPDAAAELASIAQSAPRYLGGRANTTTHN
                     RQELATLALVRLATQAPDQAAQQLEERWAALLDREERNWLWGVIGKWSALAPSERAHG
                     YFSRVTREGDLSDELLAWKVRAALRQGDWRAVLRSVDAMQDAAADDGAWLYWKARAQQ
                     ALARHDDERAAARAGFERLTAQVGRQGFYEKLALEALQRPIVAPPAPAPLTEAEREAA
                     RRHPGLGRALLAIALGLRGEGVREWNYWTNLHRPGGMGERELYAAADLACARQVWDRC
                     INTSERTRSFADFGQRFPTPHREAVQRQAQAIGLDAAYVYGLIRQESRFVTDARSGVG
                     ASGLMQVMPATARWTARRIGLSGFVPAQINDLDTNILIGTSYLKLALDDFQGSLPLAA
                     AAYNAGPGRPRAWRNGPLLDGAVWAENIPFNETRDYVKKVLSNTVDYAGLLTGQPQSL
                     AARLGRVGPLAPGQADPSMGLP"
     gene            5410..6000
                     /locus_tag="PK242_02230"
     CDS             5410..6000
                     /locus_tag="PK242_02230"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOZ92871.1"
                     /translation="MSKFDKKALRERLVRERLHLEDRLARAAALQDIMRIWLLGRPDT
                     VVGAYWPIKGEFDPLPALHRWKEDGELMDEPQRRRIGLPVIDKVHKTLTFHVWYPGCP
                     MEEDAYGIPKPKDTELIVPTLLFVPCVGYGPGGYRLGYGGGFYDRTLAALQPRPYTVG
                     LGFTQGFVDDFEPEPHDQPLDAILNDNGVVWPVMRA"
     gene            6155..7360
                     /gene="ppk2"
                     /locus_tag="PK242_02235"
     CDS             6155..7360
                     /gene="ppk2"
                     /locus_tag="PK242_02235"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOZ92872.1"
                     /translation="MSSANHQPQGSASSRKNVAPKTGKTSKTAASSRRANGDLRAVRL
                     GDTPPSLTARAIEDHVVAHATAGARDAQAQAALRLVNDPAAGTPSERASALRALLDGA
                     SPDDRKALRKVLKANGRDTLATKSNPDEELAADWRDGGYPYEHLLRRATYEQQKFKLQ
                     VELLKLQNWVKETGQRVVIVFEGRDAAGKGGTIKRFMEHLNPRGARVVALEKPSEIER
                     GQWYFQRYVQHLPTHGEIVLFDRSWYNRAGVERVMGFCSEREYQDFMHQAPEFERHLV
                     SSGIHLIKFWFSVSRAEQRRRFKERENHPLKQWKLSPIDKASLDKWDDYTRAKEAMFF
                     ETDTADSPWTVIKSDCKKRARLNAMRYVLHKLPYASKDLSQIGKLDPLIVGRAHVVYE
                     RGERPQALM"
     gene            7508..8044
                     /gene="ruvC"
                     /locus_tag="PK242_02240"
     CDS             7508..8044
                     /gene="ruvC"
                     /locus_tag="PK242_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOZ92873.1"
                     /translation="MRILGIDPGLRTTGFGVLDAEGPRLRYVASGVIQTGSAAQGNLP
                     ARLKLLFDGITELQARYTPDVAAVEIVFVNVNPQATLLLGQARGACLTALVAADLPVA
                     EYTALQMKQAVAGHGKAAKTQVQAMVQRLLGLPAAPRPDAADALGLAITHAHAGPAVA
                     RLHAAGAERTTSSMYRRR"
     gene            complement(8140..9063)
                     /gene="lpxC"
                     /locus_tag="PK242_02245"
     CDS             complement(8140..9063)
                     /gene="lpxC"
                     /locus_tag="PK242_02245"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOZ92874.1"
                     /translation="MLAQRTLKSLTRAVGVGLHSGERVELTLRPAPPDTGIVFRRIDL
                     PQPVDIPVNAQAVTDTRLASTLSVGDAKVRTVEHLMSACAGLGLDNLYIDITAEEVPI
                     LDGSASSFVYLLQSAGIEWQPAPRRFLRVLMPVEVREGEGAQLKWARLTPHHGFTLSF
                     EIDFQHPAVDATQQQVTFDMGSGRYSRDIARARTFAFSKDVEMMRASGLALGGGLDNA
                     IVMDDTRVLNEGGLRYAEEFAKHKILDAIGDLYCVGHPMLASYTAFRSGHALNNQLLR
                     TLLARRDAWDIVSFEDERQAPTGFAQLARAW"
     gene            complement(9159..10388)
                     /gene="ftsZ"
                     /locus_tag="PK242_02250"
     CDS             complement(9159..10388)
                     /gene="ftsZ"
                     /locus_tag="PK242_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOZ92875.1"
                     /translation="MSIEMIETDGFHSGTQIKVIGVGGGGSNAVEHMISRQVQGVEFI
                     CANTDAQALSRCSAGMTVQLGTSGLGAGSKPDKGREAAEAAADDIRQAIRGAHMLFIT
                     AGMGGGTGTGAAPVIARIAKDMGILTVGVVTKPFEWEGGRRLTNAEAGLTELEANVDS
                     LVVVLNEKLLEVLGDDITQEEAFAQANDVLKNAVGGIAEIINDYGFVNVDFEDVRTVM
                     GEPGKAMMGTATASGPDRARIAAEQAVACPLLEGIDLSGAKGVLVLVTASKGSLKLNE
                     SKEAMNTIRAYASPDAHTIYGAAYDDALGDEIRVTVVATGLSRQGVRRQPITVVQGGM
                     RTGTDNVPFNVPTVGQAVGGSGMPGAHAAQPDYGNVAVPSVWRTNRTQAAAKVDALSS
                     GGMDDFEIPAFLRKQAD"
     gene            complement(10555..11784)
                     /gene="ftsA"
                     /locus_tag="PK242_02255"
     CDS             complement(10555..11784)
                     /gene="ftsA"
                     /locus_tag="PK242_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOZ92876.1"
                     /translation="MAKEYKDLIVGLDIGTAKIMVVVGEVLAGGDLKLAGLGVAPSNG
                     LKRGAVVNIDATVASIQQALKEAELMADCKIGRVYTGITGSHIRGINSSGMVPIRDKE
                     VTPADVARVVEAAKAINISSDQRLLLVEPQEFVIDGQDVREPVGMSGMRLDAKVHIVT
                     GAQSAAENIIKCVRRCGLEVDQLMLNPLASSQAVLTDDERELGVACVDVGAGTTDVAI
                     FAGGAIRHTAVIPIAGDLITSDIAMALRTPTMDAEDIKVAHGCAKQLLADAETQVEVP
                     GLGDRTPRMLSRQALAGVIEPRVEEIFQLVQQVIRDSGFEELLSSGVVLTGGSAVMPG
                     MIELGEDIFLKPVRRGVPKYSRALSDMVAQPRAATVMGLMEEARAARLRGFKVAQQGG
                     GMKSAFGRLKDFIVGNF"
     gene            complement(11817..12611)
                     /locus_tag="PK242_02260"
     CDS             complement(11817..12611)
                     /locus_tag="PK242_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HOZ92877.1"
                     /translation="MTDPLPMPLDVRLMNLTTALLATGLVLACVAAALWWVLRNPAFA
                     IRQIVVTGDTAHHSAASLRATVMPRLAGNFFTLDLAATQAAFQSVPWVRKAVVQREFP
                     GRLNVTLEEHVAVGHWGEEGDHLVNRYGEVFEATADEPGERELPALRGPEGQAGTVLE
                     MYRLLNPLLEPLGAEIAELTLQGRGNWRAELDGGAVIELGHGAPAELAARLRQFAGTV
                     KEVAARHQRPVAAVEAADLRHVGGYALRLRGVGTVRPDAQPTAPRR"
     gene            complement(12608..13585)
                     /locus_tag="PK242_02265"
     CDS             complement(12608..13585)
                     /locus_tag="PK242_02265"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOZ92878.1"
                     /translation="MQPHSVQFGKVAVLMGGRSAEREVSLMSGQGVLAALRARGVDAH
                     AFDPAEQPMDELRRQGFARCFIALHGRFGEDGTVQGALELLGIPYTGAGVMTSSMAID
                     KVMTKRVWLAEGLPTPRYVLLHAGECTPEQVRAVPDALGLPLIVKPMREGSSLGVTKV
                     TSRDQMARAVAEAARLDADVLCEIFIAGDEVTCPVLGAGTRARALPVIHIVAPEGQYD
                     YQNKYFTDVTDYRVPCDLPPGEEAAVQALVLKAYRVLGCRGWGRIDVMIDGVTRQPWL
                     LEINTSPGMTGHSLVPMAARAAGIDYETLCLQLLADAALDYAPAAGARA"
     gene            complement(13596..15014)
                     /gene="murC"
                     /locus_tag="PK242_02270"
     CDS             complement(13596..15014)
                     /gene="murC"
                     /locus_tag="PK242_02270"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOZ92879.1"
                     /translation="MRHAIRHIHFVGIGGAGMSGIAEVLHNLGYVISGSDLADGAALH
                     RLSQLGIRTFVGHAAAHVEGADAVVTSTAVKPDNPEVLVARERRIPVVPRAIMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASVLAEGGLDPTFVIGGRLNSAGANARLGSGDHIVVE
                     ADESDASFLNLLPVLAVVTNIDADHMETYGHDFGRLKKAFIDFLHRMPFYGTAILCLE
                     SPAVRDIAGQLSCPVTSYGFAADAQVRAVDVRAVGGQMHFTVQRRNGVQLPDLPVVLN
                     LAGEHNVLNALAAIAVAVELNLPDAAVQKALAEFKGVGRRFQRYGEWPAPHGGRFTLI
                     DDYGHHPVEMAATLAAARGAFPGQRLVLAFQPHRYTRTRDCFEDFVAVLGQADHVLLT
                     EVYAAGEEPIVAADGRSLARALRVAGRVEPVFVDDVAELPRVVAEQARAGDVVLCMGA
                     GSIGGVPAKIVDMLQKTEHNPD"
     gene            complement(15011..16075)
                     /gene="murG"
                     /locus_tag="PK242_02275"
     CDS             complement(15011..16075)
                     /gene="murG"
                     /locus_tag="PK242_02275"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOZ92880.1"
                     /translation="MKQKCALVMAGGTGGHIFPGLAVADGLRARGWRVHWLGAPGSME
                     QQLVPPRGYPFEAIDFGGVRGKGLVTLALLPLRLLRAFAQSIGVIRRVRPDVLVGLGG
                     YITFPGGLMGVALGKPLVLHEQNSVAGMANRVLAGVADRVFTAFPQVLRKGRWIGNPL
                     RPEFAAQPAPAQRFAGRHGPLRVLVVGGSLGAQALNDVVPRALALLPPDARPQVRHQS
                     GARQIDALRANYATAGVQAELLPFIDDMAQALAEADLVICRAGATTVTEIAAIGAAAL
                     FVPFPHAVDDHQTTNARFLVDQGGGWLVRQSELSPESLAQMLQNMERSALMERALKAK
                     TFQKTQAVEEMVAACEELAA"
     gene            complement(16072..17319)
                     /gene="ftsW"
                     /locus_tag="PK242_02280"
     CDS             complement(16072..17319)
                     /gene="ftsW"
                     /locus_tag="PK242_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOZ92881.1"
                     /translation="MPSDGGKKRRAARARQMDELPVRIGGMPYARTTSTPTRVLGFDQ
                     SLIWVCVALLAWGLVMVYSASIALPDNPRFSRYAPGHFVLRHAFWIVLSFVAALIAFQ
                     VRMDWWEKVAPWLFVSALGLLIAVLIPHVGTVVNGARRWISLGPINFQPSELAKMAVL
                     LYAADYMVRKMDVKERFFRAVLPMGVAVGVVGLLLLAQPDMGAFLVIAVIAMGILFLG
                     GVNARMFFLIAAILVVAFAVMIWASPWRRERIFAYLDPFSEDHALGKGYQLSHALIAI
                     GRGEIFGVGLGGSIEKLHWLPEAHTDFLLAVIGEEFGLVGLVCVIFLFFWLTRRIMHI
                     GRQAIALDRVFSGLVAQGIGLWLGFQAFINIGVNLGALPTKGLTLPFMSYGGSAILAN
                     MVAIAIVLRVDVENRRLMHGGRA"
     gene            complement(17334..18857)
                     /gene="murD"
                     /locus_tag="PK242_02285"
     CDS             complement(17334..18857)
                     /gene="murD"
                     /locus_tag="PK242_02285"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOZ92882.1"
                     /translation="MNLLQDRHVLVLGLGLSGLAMARWCARQGASVTVADTRAEPPQL
                     AALRECLPAARFICGALDATLLEDASVRAVYRSPGLAPASIATLVQAARERGLPVGGE
                     LELFMQALQELGQARGYQPRLLAVTGTNGKTTVTALTGQLVERAGPSVAVAGNIGPSL
                     LDTLQARLDGEALPEVWVLELSSFQLDGVAGFEPTAATVLNVTQDHLDWHGDMASYAA
                     AKARIFGEHGVMVLNRNDPVVIGMRPAVPARSFKGPRPTPRRVVTFGADLPDAPGDFG
                     IEMVSGMAWLVRAQEADETQRRRKDAELELHLQRLMPADALRIRGRHNAVNALAALAL
                     ASSAGGALGPMLYGLREYRGEPHRVESVGVLEGVEYFDDSKGTNVGATVAALEGLGAE
                     RRLVVILGGDGKGQDFSPLAAPVSRFARAAVLIGRDAPQVHAALQQAGVELLSAEDMG
                     AAVALARSRAQAGDAVLLSPACASLDMYANYVARADAFRAAVREQALAAGVELEVLA"
     gene            complement(18861..20039)
                     /gene="mraY"
                     /locus_tag="PK242_02290"
     CDS             complement(18861..20039)
                     /gene="mraY"
                     /locus_tag="PK242_02290"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOZ92883.1"
                     /translation="MLPSLATWLQTLSPDFGFFRVFQYLTFRALMAAMTALVIGIALG
                     PWVIRRLTALKIGQPVRGYAMQSHLVKSGTPTMGGVLILLSIAVSTLLWFDWSNRFVW
                     IVMLVTFGFGAIGWVDDWRKVVNKDPEGMRSREKYFWQSVIGLVAAFYLVFSISEASN
                     ARVWALFVNWVSSGFSIDLPPAAGLLVPFFKEINYPLGVLGFVILSYLVIVGSSNAVN
                     LTDGLDGLAIMPVILVGSALGVFAYVTGNAVYARYLLIPHIPGTGELLIFCAALAGAG
                     LAFLWFNAHPAEVFMGDVGALALGGALGTIAVIVRQEIVLAIMGGIFVVEALSVMLQV
                     GWFKYTKRRYGEGRRLLKMAPLHHHFEKNGWAETKVVVRFWIITMLLCLAGLSSLKLR
                     "
     gene            complement(20029..21438)
                     /gene="murF"
                     /locus_tag="PK242_02295"
     CDS             complement(20029..21438)
                     /gene="murF"
                     /locus_tag="PK242_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOZ92884.1"
                     /translation="MSAMALSLQELAGWLTGARLVGDGAVRCARVHSDTRSLQAGDLF
                     VALKGERFDAASFLGEAAAQGAVAALCDERAEARLVAAGLPGVLVPDVRLALGELAHR
                     WRRRFDGPLIAVTGSNGKTTVTQMVASIFRAWQGTAALSTEGNLNNDIGLPLTLLRLR
                     PQHRVAVVELGMNHPGEIAQLAAIAAPTVALVNNAQREHQEFMASVEAVARENGAVLT
                     ALPADGVAVFPAADAFSPLWRSLAGGRRVLDFGAGAAVHLSQATWAVDHWAVSVATPV
                     GQFDGALHVAGRHNLHNALAATACALAAGAPLAAIARGLGEFRPVKGRSRTVLLRQRG
                     RAITLVDDTYNANPDSVRAVIDVLAELPGPRLLVLGDMGEVGEQGPAFHAEVGAYARQ
                     RGVEHLLAHGPQAIHAAMAFGGGRHFDDIGALIDAVRTHLPQCATLAVKGSRFMRMER
                     VVEALSAATGPTAEDAHAA"
     gene            complement(21435..22940)
                     /locus_tag="PK242_02300"
     CDS             complement(21435..22940)
                     /locus_tag="PK242_02300"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOZ92885.1"
                     /translation="MALAQCHHPTEAARWLRARVTGRLHADSRQVEPGDGFIAWPGAA
                     VDGRRFVAAALAQGARACLVEHEGVQAFGFEQPQVASYAHLKADTGPIAAAYYGEPSQ
                     ALQVVAVTGTNGKTSTAWWTSWALSKTELPTLSPCALVGTLGVGLPQALQVTGMTTPD
                     PVLLQRNFRELVDAGVRACAIEASSIGIAERRLDGTAIRVAVFTNFTQDHLDYHGSMQ
                     AYWQAKAALFDWPGLQAAVIHADDPRAGELVAKAQARGLDVWTVARERPARLRATDIA
                     YADAGLHWVVCEGEQRAALRTTLVGEYNIDNLMGVLATLRALGVPLQQAVAACTDLPA
                     VPGRMERIGLPGAPLAVVDYAHTPDAIDKALRALRPLAAQRGGRLWCVFGCGGNRDAS
                     KRPLMAQAAEAAADRLVVTSDNPRHEDPQAIIDVIGAGLRRPGAARFEVDRAQAIAAA
                     LTEAQPADVVLIAGKGHEDYQEVRGQRTPFSDQAVARRVLQRRVATGEAPA"
     gene            complement(22942..24678)
                     /locus_tag="PK242_02305"
     CDS             complement(22942..24678)
                     /locus_tag="PK242_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOZ92886.1"
                     /translation="MRDIRYTSSPLLASKTPVWRSKFIVAMLALAFLVLIGRAAYVQV
                     FGNAFFQRQGQVRFARTLELPASRGRILDRNGLLLASSVVAPSIWAIPEDVDRDPARL
                     RELARLLSMPLAELNKKLSDEDKTFVWLKRQVDESVARQIAELKLAGIYQRKEYKRKY
                     PEGEAAAHVVGFANVENQGQEGAELSFEKRLAGRAGSRRVIKDRLGRVVEAVGEEVPP
                     VDGQDVQLSIDSKVQFFAYQKLRDAVTSNKARSGSVVVLDAQTGEVLALANYPSYSPD
                     NRRHLSGAQLRNIAVTDTFEPGSTMKPVTVAMALEAGKVTPQTLIATAPGSYKLDKFT
                     IRDTHNYGTLTVEGVVQKSSNVGSLKIAQRLSAQEMWETYTALGYGRKPELAFPGAAA
                     GRVRHWKSWRPVEQATMSYGYGLSASLFQMAHSYTAFSHDGRVIPATLLKAPEGASVE
                     GVRVFSEKNARAVRKMLALAAGPGGTGQRAQTVGYSVGGKSGTARKQVGKGYASNRYR
                     AWFTGMAPIEQPRVIVAVMIDEPSNGVYYGGAVAAPVFSEVVQQTLRIMGVQPDMAVK
                     PAIVAEQVEESF"
     gene            complement(24675..24980)
                     /gene="ftsL"
                     /locus_tag="PK242_02310"
     CDS             complement(24675..24980)
                     /gene="ftsL"
                     /locus_tag="PK242_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOZ92887.1"
                     /translation="MTRLSIVLLVAVMVSALYVVRVQYDSRRLFTELDRASAEAHRLE
                     TERARLEVEKRAEATSLRVEKLARDKLAMRSITPAITEYVTSSLALPAASAPQGERP"
     gene            complement(24977..25909)
                     /gene="rsmH"
                     /locus_tag="PK242_02315"
     CDS             complement(24977..25909)
                     /gene="rsmH"
                     /locus_tag="PK242_02315"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOZ92888.1"
                     /translation="MSAWSHTTVLLNEAVDALVTDPRGRYVDATFGRGGHARQILRRL
                     APEGRLTAFDKDPEALAEAAHIRDARFAIRHQGFAALGEWPPHSVAGVLMDLGISSPQ
                     IDNPARGFSFRADGPLDMRMDPTRGQSAADWLAHADERQIAQVIRDYGEERFAVAIAK
                     AIVARRQAGRAVASTLELAELVAGAVKTREPGQNPATRTFQAVRIFVNAELEELEQAL
                     EAAVQVLQPGGRLVVISFHSLEDRIVKQFIARHARAPVDRRAPFAEPAPPRLRALGKS
                     RPGAAELAANPRARSAIMRVAERTAAPTHHGGRA"
     gene            complement(25922..26350)
                     /gene="mraZ"
                     /locus_tag="PK242_02320"
     CDS             complement(25922..26350)
                     /gene="mraZ"
                     /locus_tag="PK242_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOZ92889.1"
                     /translation="MFQGASSLSLDAKGRLSVPTRHRDVLAATASGQLTITKHPDGCL
                     MVFPRPEWEKFRERLIQLPMSAQWVKRIFLGSAMDVELDGTGRVLVSPELRQAAGLTR
                     EVMLLGMGRHFELWDKLTYEAKEAQAMQAGLPGSFEDFTF"
BASE COUNT         4220 a         9634 c         9051 g         3806 t
ORIGIN      
        1 tctgcagcgc ctggcccacc tgcagcgcgt ccacctgggc aaagatctgc tggtgacgct
       61 gatacggcgg aatgttgcgc acgtcgattt cgacgagggt ggcggcgctc atggatcaac
      121 ctttctgaaa gagctgcgaa gaagcgggtt gtgcccggac gcccgcggcg cccggaacgg
      181 aaaaccgggg gccggcgctc agccgtcctt gccatccagc cgcgtgcgcg tcagccaggg
      241 cacatagatc accaggtaga tggcaaaggc cagcgcccac agcccggcgg ccgaatccag
      301 cgcccgcacg taccaggccg gcaccaccgc cggcaccagc acgcgcagca cggtggccag
      361 catcaccagc gcgtaggcca cgacctcgcc gcgcgaggcc tgcagcggcc ggccggtgtg
      421 gccgcgcgcg gtgcgcgtga tcatgccgat gatcagcccg ccgatcacgc cgacgccgaa
      481 cgcatgcacc gccagcgacg gcgccaccca gcccggctgc gccagcgcca gcagcgcaaa
      541 gccgaccggc atccaggcgt aggaaaaatg caggatccac aggatcgggc ggcgcagcgt
      601 cacacgcgga tgccacttcc acaggcgcag cgcgtgcagc gcggcagaca gggcgctggc
      661 gggcagcacc agccaggccg gcgcacccag cacccacagc gccagcgtga cggcggtgac
      721 ggccagcagc gtcagctcga agcggcgcga cacctggatc accaggccgg gcgtcacgtt
      781 cttggtgaac atgggcacca cgcggccggt catgatgctg accaccatca ggatcagcgc
      841 cagctcggcg tacagggccg acatggcggg cacggcgatc agccccagca ccgacaggtg
      901 gaacaccgcg ttggccagcg ccatcgccag cagcagcgaa atcagcggga tgttgcgccg
      961 gttgccggcg cggatcagca cgcgcagcag gaagaacgcc accgccggca gcagcagcag
     1021 atcgagcacg gcgaacagcg ggtacggcgc cagcacggcc gccagccgcg ccgccagcca
     1081 caacagcgcc agcaggccca gcaccggccc gcgcggcgtc tccagcccgg tccaggcgcg
     1141 cacggcggtc agcagaaagc cgatcaccac gccggcggca aagccgaaca gcatttcgtg
     1201 cgcgtgccac agcaggatcg gaaacggcag ccgcagcggc atgtggccca ggaaggccgc
     1261 gatccacagc gggatcgaca cgcaggccag cagcgccgtg cccaggtaga agggccgaaa
     1321 gcccagccgc agcagcggcc agcccggcag cgcggccggg ggctgcgcgc ggtcatcgat
     1381 cgacatgagg ggagcggaac gctgggtcat ggagggctgg ccggcggcag cggcgaaggg
     1441 gccggcggca tcggaatgtc aaaagctgca ttttgcatga atctttaagg cctgccaaat
     1501 ccggggccgg gcgggcgccg gcgcgccccg gttcaggccg acgccggtcg ggccgcgcgg
     1561 tacggttctt cgaacggcag gaagtcctgc tccgccagcg cgccttcgat ccaggcggcg
     1621 acgccgggca gcgcgcacag gcgcgccatg taggcggcca cgtcgtccgg caccggcagg
     1681 gcgtaggttt tcagccgcat caccaccggg gcgtagaagg cgtcggcgat gccgaagcgg
     1741 ccgaacagca tcggcccgcc gtgcatggcc agcagttcct gccacatctc cacgatgcgc
     1801 gccacgtcgg cgcgcacgtc gggccggtcg cgccagatga tggcgccggc ctcgggcagc
     1861 tgcgcttcga tgttcatgcc gcaggcgctg cgcagcgcgc caaagccgct gtgcatttcg
     1921 gcgcacacgc tgcgggcgcg ggcgcgctcg ggggcgctgc gcggccacag gtgcagttcg
     1981 ggaaatttct cggccaggta ctcggcgatg gccagcgtgt cccacaccac cagctcgccg
     2041 tcggtgagca ccggcacctt gccggccggg ctgatgcggc ccaggcgctg cttgaactgc
     2101 gagtcgcccg agaacgagtc gaagcgcagc tgcacttcct cgaacgcgat gccggcctgc
     2161 ttcatcagca cccaggggcg catggaccag gacgaatagt tcttgttgcc gatgtagagc
     2221 ttgagcatgc ggggcccttc cagaaattct cagcgcccgg cgctgcgccg gtaggcgtcc
     2281 aggcgcgcgg ccggatggtc ggcaccgacg atcatggggc cgacggccga cagcggcgcc
     2341 ggcgtgatgc ccagctcggc caggccgggc agctggccgc tggcgacgtt gtccacccgc
     2401 atcgaatcga ggttgtcgcg gctcatcagc ggttcgccgg gcaagcattc catggccagc
     2461 gcctgcagcc gtcccagcgc cgccggcagg ccgacgatcg ggcgcccgcg cccgccgcgc
     2521 acgccggccc acatgccggc cgcgcgcacc agctcgcgca gcgtccagac ttcgggcccg
     2581 cacagctcgt aggtgcgccc ggcggtggtg tcgtcctgca ggcagcgcac cagcgcctgc
     2641 gccacgtcgc gcacccacac cggctgaaag cgcgcctcgg cgctggccag cgggatcagc
     2701 ggaaacagcc gctgcagccc ggcgaacagg ttcagcaggt ggtcgccgct gccgtagatg
     2761 acgctggggc gcagcagcgt cagcgccagc gcgccgtcga cggcggcctg ctgcagcacg
     2821 gcttcgccct gggccttgct gcgctggtag cgcgacgggc catcggcggc cgcgcccagg
     2881 gcgctgatgt gcaccagccg ccgcacgccg ctggcggcgc aggcggcggc gatgcggcgc
     2941 ggcagctcca catgcacctg ctcgaagcgc cgcgcatcgc cgtgcagcac ggcgatcagg
     3001 ttgaccaccg cctggtgacc ggccagcagc gcgcccaggt gcgccgggtc ggtgacgtcg
     3061 gccggcacca catcgacacg cggcaggctc cacagccagg gcgcggcgtc gcgccgccgc
     3121 gtgggcacgg tcacgcgcac gcccagccgc tgcaggtgtt cgatcaggtg gcggccgaca
     3181 aaccccgaac cgcccagaac caggatccgc ttgcccatgg aatgactcct gatttgatag
     3241 ctgtcggcgc actacagaag cgcgccgaag cccgattgaa cctaaaatcg tgactcaggg
     3301 caagcccatg ctcgggtcgg cctgccccgg cgccagcggc cccacccgac ccagccgcgc
     3361 cgccagcgac tgcggctgtc cggtgagcag gccggcatag tcgacggtgt tggaaagcac
     3421 tttcttgacg tagtcgcggg tttcgttgaa ggggatgttt tcggcccaca ccgcgccgtc
     3481 cagcagcggg ccgttgcgcc aggcgcgcgg tcggccgggg ccggcgttgt aggcggcggc
     3541 ggccagcggc agcgagccct gaaagtcgtc cagcgccagc ttcaggtagc tggtgccgat
     3601 caggatgttg gtgtccaggt cgttgatctg cgcgggcacg aagccgctca ggccgatcct
     3661 gcgcgccgtc cagcgcgcgg tggcgggcat gacctgcatc aggcccgagg cgccgacgcc
     3721 cgagcgcgcg tccgtcacga agcggctttc ctgccggatc aggccataca cataggcggc
     3781 gtccagcccg atggcctgcg cctggcgctg cacggcttcg cggtgcgggg tcggaaagcg
     3841 ctgcccgaag tcggcgaagc tgcgcgtgcg ctcgctggtg ttgatgcagc ggtcccagac
     3901 ctggcgcgcg caggccagat cggcggcagc gtacagctcg cgctcgccca tgccgcccgg
     3961 gcggtgcagg ttggtccagt aattccattc gcgcacgcct tcgccgcgca ggcccagggc
     4021 gatggccagc aacgcgcggc cgaggccggg gtggcgacgc gccgcttcgc gctcggcctc
     4081 ggtcagcggc gccggggcgg gcggcgccac gatgggccgt tgcagcgcct ccagcgccag
     4141 cttttcgtag aagccctgac gcccgacctg ggccgtcagg cgttcgaagc cggcgcgcgc
     4201 ggcggcgcgc tcgtcgtcgt ggcgcgccag cgcctgctgg gcacgcgcct tccagtacag
     4261 ccaggcgcca tcatcggccg ccgcgtcctg catggcgtcg acgctgcgca gcacggcgcg
     4321 ccagtcgccc tggcgcaggg cggcgcgcac cttccaggcc agcagctcgt cgctcaggtc
     4381 gccctcgcgc gtgacgcggc tgaagtagcc gtgcgcgcgc tcggacggcg ccagcgccga
     4441 ccacttgccg atcacgcccc acagccagtt gcgctcctcg cggtccagca gcgcggccca
     4501 gcgctcttcc agctgctgcg cggcctggtc gggagcctgc gtggccagcc gcaccagcgc
     4561 cagcgtggcc agctcctgcc gattgtgcgt ggtggtgttg gcgcgcccgc ccaggtagcg
     4621 cggcgcgctc tgggcgatgc tggccagctc ggcggcggcg tcgggcgcgt ccagcgccac
     4681 ggcggcgcgt gccagcgccg gccggttggc ctccatcgcc aggcgggcct tcttccacag
     4741 cacctggtag ggcagcagcc cggtctggtg cagcaggccg gcggcgtaca ggcagccgtc
     4801 gtcggcgtcg cgctgcgcca gccagtcgcg ccgcacctgc tcgaccgcct gcgggccggg
     4861 cgccaggccg gcggcgaggt cggccgacac cgcgtggcag cgcagttcgc ggtcgtcgcg
     4921 catgcggtag cgcgggtatt cggcgcgaaa cgccgcccag tcgcgccgct tgcccagcag
     4981 cagcagccag tcgttgcgca ggcggtcttc ctggtagctg ccggcatagc gggccaggaa
     5041 atcctgcacc tcggtcgcgc cggcgtcttc caggcgcgcc ttgagttccc agtaggcggc
     5101 ccagggctcc agcgcatggc cgcgcgcctg gggcagcagg gccgtcagcc gcgccttgtc
     5161 gccgcgccgg tgggcgtcgc gcatgtcgag caggaccgca tcgcgcgccg ccgcgtcggc
     5221 gggcagggcc agcatcggca ccaccgggct ggcgtcggca gcctgcgacg aggcggcggt
     5281 cggctccggc ggctgcggcg ccgccgccgg ttgcgcccag gccaccgtca tgcccagcag
     5341 cgccaggccg gccccagccc gtgcggcgcg tgaggatgtc agaatcgttg gccaacgcat
     5401 cgaccgatta tgagcaagtt cgacaagaaa gccctgcgcg aacgcctggt gcgcgagcgc
     5461 ctgcatctgg aggaccggct ggcgcgggcc gcggcgctgc aggacatcat gcgcatctgg
     5521 ctgctgggcc gcccggacac ggtggtgggc gcctactggc cgatcaaggg cgagttcgac
     5581 cccctgcccg ccctgcaccg ctggaaggaa gacggcgagt tgatggacga accacaacgc
     5641 cgccgcatcg gactgccggt gatcgacaag gtgcacaaga cgctgacgtt ccacgtctgg
     5701 taccccggct gtccgatgga agaagacgcc tacggcattc ccaagcccaa ggacaccgag
     5761 ctgatcgtgc ccaccctgct gttcgtgccc tgcgtcggct acgggccggg cggctaccgg
     5821 ctgggctatg gcggcggctt ctacgaccgc acgctggcgg cgctgcagcc gcggccatac
     5881 acggtgggac tgggtttcac gcagggcttc gtcgacgact tcgagcccga gccgcacgac
     5941 cagccactgg acgcgatcct gaacgacaac ggcgtggtgt ggccggtcat gcgagcgtga
     6001 cacgctgcac ggcgccggca cgcgccactc acgtttcttg acctacctca agctgaatcc
     6061 cagtacccgg atggaaagta cgggtattgg ggcaacacgc cgccgcaggt ggctggcccg
     6121 tccccttgtc gaaccttcat cgccccaccc ttccatgagc tccgccaacc accagcccca
     6181 gggttctgcg tccagccgca agaacgtcgc tccgaaaaca ggcaagacca gcaagaccgc
     6241 cgccagctcc cggcgggcca acggcgacct gcgcgcggtg cgcctgggcg ataccccgcc
     6301 ctcgctgacg gcgcgggcca tcgaagacca cgtggtcgca cacgccaccg ccggggcccg
     6361 cgacgcccag gcgcaagccg cgctgcgcct ggtcaacgac ccggccgccg gcacgccgag
     6421 cgagcgcgcc agcgccctgc gcgcgctgct cgacggcgcc tcgcccgacg atcgcaaggc
     6481 cctgcgcaag gtgctcaagg ccaatggacg cgataccctc gcaaccaagt ccaatcccga
     6541 cgaggaactg gcggcggact ggcgcgacgg tggctatccc tacgaacacc tgctgcgtcg
     6601 cgccacttac gagcaacaga agttcaagct gcaggtcgaa ctgctcaagt tgcagaactg
     6661 ggtcaaggag accggccagc gcgtggtgat cgtgttcgaa gggcgcgacg ccgccggcaa
     6721 gggtggcacc atcaagcgct tcatggagca cctcaatccg cgcggagcgc gtgtcgtcgc
     6781 cctggaaaaa cccagcgaga tcgagcgcgg gcagtggtac tttcagcgct acgtgcagca
     6841 cctgcccacc catggcgaga tcgtgctgtt cgaccgcagc tggtacaacc gcgccggcgt
     6901 cgaacgcgtg atgggttttt gcagcgaacg tgagtaccag gacttcatgc accaggcgcc
     6961 cgagttcgaa cgtcacctgg tcagcagcgg catccacctg atcaagttct ggttctcggt
     7021 cagccgtgcc gagcagcggc gtcgcttcaa ggagcgcgaa aaccaccccc tcaagcagtg
     7081 gaagctcagc cccatcgaca aggccagcct cgacaagtgg gacgactaca cacgcgccaa
     7141 ggaggccatg ttcttcgaga ccgacaccgc cgactcgccc tggacggtca tcaagagcga
     7201 ctgcaagaag cgcgcccgcc tcaacgccat gcgctacgtg ctgcacaagc tgccctacgc
     7261 cagcaaggac ctgtcgcaga tcggcaagct agacccgctg atcgtggggc gcgcccacgt
     7321 ggtgtacgag cgcggcgaac gcccgcaggc gctgatgtga aggccgacgg gcgctggccg
     7381 ggtcgccccg gattctggag ctatcgcagc acagcgccca agggtagcgc gcgagcagct
     7441 atcattatga aagcgccctg ctgatcgaca accggccgca tccactccgg ggcgcgacac
     7501 ccaccccatg cgaatcctcg gcatcgaccc cggcctgcgc accaccggct ttggcgtgct
     7561 cgacgccgag ggcccgcgcc tgcgttacgt cgccagcggc gtgatccaga ccggcagcgc
     7621 cgcacagggc aacctgccgg cgcggctcaa gctgctgttc gacggcatca ccgagctgca
     7681 ggcgcgctac acgcccgacg tggcggcggt cgagatcgtg ttcgtcaacg ttaaccccca
     7741 agccacgctg ctgctgggcc aggcgcgcgg cgcctgcctg accgcgctgg tggccgccga
     7801 tctgccggtg gccgaataca ccgcgctgca gatgaaacag gccgtcgccg gccacggcaa
     7861 ggccgccaag acccaggtgc aggccatggt gcagcgtctg ctgggcctgc ccgccgcacc
     7921 gcgccccgac gccgccgacg ccctgggcct ggccatcacc cacgcgcacg ccggccctgc
     7981 cgtcgcccgc ctgcacgcgg cgggcgccga gcgcaccacc agcagcatgt accggcgccg
     8041 ttgagctgaa aattgccttt ggcgcgcatg cagtaagcgc caacagctac aatttcagta
     8101 gcgtattgaa gctgcgcaac gatggttcgc cggcacggct caccaggcgc gggccaactg
     8161 ggcgaagccg gtcggggcct ggcgctcgtc ttcgaagctg acgatgtccc aggcatcgcg
     8221 gcgcgccagc aaagtgcgca gcagctggtt gttcagggca tgcccggagc ggaaggcggt
     8281 gtaactggcc agcatggggt ggcccacgca gtacagatca ccgatggcgt cgaggatctt
     8341 gtgcttggcg aattcctcgg cgtagcgcag gccgccttcg ttcagcaccc gggtgtcgtc
     8401 catgacgatg gcgttgtcca gcccgccacc cagggccaga ccgctggcgc gcatcatttc
     8461 cacgtccttg ctgaaggcga aggtgcgggc gcgcgcaatg tcgcggctgt acctgcccga
     8521 acccatgtcg aacgtgacct gctgctgcgt ggcatcgacg gccgggtgct ggaagtcgat
     8581 ctcgaagctg agtgtgaagc cgtggtgcgg cgtcaaccgg gcccacttga gctgcgcgcc
     8641 ttcgccttcg cgcacctcca ccggcatgag cacgcgcaaa aacctgcgcg gcgccggctg
     8701 ccattcgatg ccggcgctct gcagcaggta gacgaaggac gaggccgagc cgtcgaggat
     8761 gggcacttcc tcggccgtga tgtcgatgta cagattgtcc agaccgaggc cggcgcaggc
     8821 cgacatcagg tgctcgacgg tgcgcacctt ggcatcgccc accgacagcg tagacgccag
     8881 ccgggtgtcg gtgacggcct gcgcgttgac cgggatgtcc accggctgcg gcaggtcgat
     8941 gcggcgaaac acgatgccgg tgtccggcgg cgccggccgc agcgtcagtt ccacgcgctc
     9001 gccgctgtgc agccccacac ccaccgcgcg ggtgagcgac ttgagcgtgc gttgggccag
     9061 catagggggg cgattttaca aagccatcgg caggcgcgcc gggcgtggtg cagggcgcgg
     9121 gcggctggcg ccacacgccc cgcctgcggc ctcgacgctc aatcggcctg cttgcgcagg
     9181 aaggccggga tctcgaagtc gtccatgccg ccggaggaca gcgcgtccac cttggccgcc
     9241 gcctgcgtgc ggttggtgcg ccacacgctg ggcaccgcca cgttgccata gtcgggctgg
     9301 gcggcatggg cccccggcat gcccgacccg cccacggcct gaccgaccgt gggcacgttg
     9361 aacggcacgt tgtcggtgcc ggtgcgcatg cccccctgca ccacggtgat gggctggcga
     9421 cgcacgccct ggcgcgacag accggtggcc accacggtga cgcggatctc gtcgcccagc
     9481 gcatcgtcgt acgccgcgcc atagatggtg tgggcgtcgg gcgaggcata ggcgcggatg
     9541 gtgttcatcg cctccttgga ctcgttcagc ttgagcgatc ccttggatgc ggtgaccagc
     9601 accagcacgc ccttggcgcc ggacaggtcg atcccctcca gcagcgggca ggccaccgcc
     9661 tgctcggcgg cgatgcgagc gcggtcgggc ccgctggcgg tggcggtacc catcatggcc
     9721 ttgcccggct cgcccatcac ggtgcgcacg tcctcgaaat cgacgttgac gaagccgtag
     9781 tcgttgatga tttcggcgat gccgccgacg gcgttcttca gcacgtcgtt ggcctgggca
     9841 aaggcctctt cctgggtgat gtcatcgccc agcacttcga gcagtttttc gttcagcacc
     9901 acgaccagcg aatcgacgtt ggcttccagc tcggtcaggc cggcctcggc gttggtcagg
     9961 cgccgaccgc cctcccattc gaacggcttg gtgaccacgc cgacggtcag gatgcccatg
    10021 tccttggcga tgcgggcgat caccggcgcg gcgccggtgc cggtgccgcc ccccatgccg
    10081 gcggtgatga acagcatgtg cgcgcctcgg atcgcctgac ggatgtcgtc ggccgcggcc
    10141 tcggccgcct cgcggccctt gtcgggcttg ctgccggccc ccaggccgct ggtgcccagc
    10201 tgcaccgtca tgccggcgct gcagcggctg agcgcctgcg catcggtgtt ggcgcagatg
    10261 aactccaccc cctgcacctg gcgcgagatc atgtgctcga cggcgttgct gccgccccca
    10321 ccgaccccga tcaccttgat ctgcgtgccg gaatggaaac cgtcggtttc aatcatttcg
    10381 atgctcatgc tggactcctt ttcttgccta cctgctttgg attgcgaaca ctggaatggg
    10441 gacgaaggcg ccacgcggtg gctgcgccgg cacgcctgcg ccagggcggg ccggtgctct
    10501 gccagcggtg ggaagcggcg tgcgccggcg atgcctgatc ctgcgtgtgt ttcatcagaa
    10561 gttccccacg atgaaatcct tcagacgacc gaaggcgctc ttcatgccgc cgccctgctg
    10621 cgccaccttg aagccgcgca ggcgcgccgc gcgggcttct tccatcagac ccatcacggt
    10681 ggcggcacgt ggctgcgcca ccatgtcgga cagggcgcgc gaatacttgg gcacgccgcg
    10741 ccgcaccggc ttgaggaaga tgtcctcgcc caactcgatc atgccgggca tcaccgcact
    10801 gccgccggtc agcaccacgc cggaagacag cagttcctcg aaaccggagt cgcggatcac
    10861 ctgctgcacc agctggaaga tctcctcgac gcgcggctcg atcacgccgg ccagcgcctg
    10921 acgcgacagc atgcgcggcg tgcgatcgcc cagccccggc acctcgacct gggtctcggc
    10981 gtcggccagc aactgcttgg cgcagccgtg cgccaccttg atgtcttcgg cgtccatggt
    11041 cggcgtgcgc aacgccatgg cgatgtcgct ggtgatcaga tcgcccgcga tcgggatcac
    11101 cgccgtgtgg cggatcgcgc caccggcaaa gatggcgacg tcggtggtgc cggcgcccac
    11161 gtccacacag gccacgccca gctcgcgctc gtcgtcggtc agcaccgcct ggctggacgc
    11221 cagcgggttg agcatcagct gatccacctc cagcccgcag cggcgcacgc acttgatgat
    11281 gttctcggcc gcgctctgcg cgcctgtgac gatgtgcacc ttggcgtcca gccgcatgcc
    11341 gctcatgccg accggttcgc gcacgtcctg gccgtcgatc acgaattcct gcggctcgac
    11401 cagcagcagg cgctggtcgc tcgagatgtt gatggccttg gccgcctcga ccacgcgcgc
    11461 cacgtcggcg ggggtcactt ccttgtcgcg gatcggcacc atgccgctgg aattgatgcc
    11521 gcggatgtgg ctgccggtga tgccggtgta gacgcggccg atcttgcaat cggccatcag
    11581 ttcggcttcc ttgagcgcct gctggatgct ggccacggtg gcgtcgatgt tgaccacggc
    11641 gccgcgcttg aggccgttgc tgggcgccac ccccaggccg gcgagcttga gatcgccgcc
    11701 ggccagcact tcgcccacca ccaccatgat cttggcggtg ccgatgtcca ggccgacgat
    11761 caggtccttg tactcttttg ccatgtaggt ttcgctcttg ttctcgtctt gtcgcctcaa
    11821 cgccgggggg ccgtcggctg ggcgtcgggg cgcaccgtgc cgaccccgcg caaccgcagc
    11881 gcgtagccgc ccacatgccg cagatcggcg gcctcgacgg ccgccaccgg ccgctgatgg
    11941 cgcgccgcca cttccttgac cgtgccggca aactgccgca ggcgggccgc cagctcggcc
    12001 ggcgcaccgt gaccgagctc gatcaccgcg ccaccgtcca gttcggcacg ccagttgcca
    12061 cgcccctgca gggtcagctc ggcgatctcg gcccccagcg gctccagcag cgggttcagc
    12121 aggcggtaca tctcgagcac cgtgccggcc tgcccttcgg gaccacgcag ggcgggcagc
    12181 tcgcgctcgc cgggttcgtc ggccgtggcc tcgaacacct cgccgtaccg gttgaccagg
    12241 tgatcgccct cttcgcccca atgcccgacc gccacgtgct cttccagcgt gacgttcagt
    12301 cgccccggga actcgcgctg caccaccgcc ttgcgcaccc agggcacgga ctggaacgcc
    12361 gcctgcgtgg ccgccagatc gagcgtgaag aagttgcccg ccaggcgcgg catgacggtg
    12421 gcgcgcaggc tggcggcgct gtggtgcgcc gtgtcgccgg tgaccacgat ctgccgaatg
    12481 gcgaacgccg ggttgcgcag cacccaccac agggccgccg ccacgcaggc cagcaccaga
    12541 ccggtggcca gcagcgcggt ggtcaggttc atcagcctga cgtccagcgg catgggcagc
    12601 gggtcggtca tgcgcgtgct cccgctgccg gggcgtagtc cagcgcggca tcggccagca
    12661 gctgcaggca cagggtttcg tagtcgatgc cggccgcgcg cgcggccatc ggcaccagcg
    12721 aatgcccggt catgccgggc gaggtgttga tttccagcag ccagggctgg cgcgtgacgc
    12781 cgtcgatcat cacgtcgatg cgtccccagc cgcggcagcc cagcacgcgg taggccttga
    12841 gcaccagcgc ctggacggcg gcctcctcgc cgggcggcag gtcgcagggc acgcggtaat
    12901 cggtgacgtc ggtgaagtat ttgttctggt agtcgtactg gccttcgggg gccacgatgt
    12961 ggatcaccgg cagcgcgcgc gcccgggtgc cggcacccag caccggacag gtgacttcgt
    13021 cgccggcgat gaaaatctcg cacaacacgt cggcgtccag gcgcgcggcc tccgccaccg
    13081 cccgcgccat ctggtcgcgg ctggtgacct tggtgacgcc gagcgacgaa ccttcgcgca
    13141 tcggcttgac gatcagcggc agccccagcg catcgggcac cgcacgcacc tgctcgggcg
    13201 tgcattcgcc ggcgtgcagc agcacgtagc gcggcgtcgg cagaccttcg gccagccaga
    13261 cgcgcttggt catcaccttg tcgatggcca tgctggaggt catcaccccg gcgccggtgt
    13321 agggaatgcc cagcagctcc agcgcgccct gcaccgtgcc gtcttccccg aaacgcccgt
    13381 gcagggcgat gaagcagcgc gcaaaaccct ggcgcctgag ctcgtccatc ggctgctcgg
    13441 ccggatcgaa ggcatgcgcg tcgacgccgc gcgcgcgcag cgccgccagc acgccctgac
    13501 cggacatcag ggacacctcc cgctcggccg agcgaccgcc catcaggacc gcgacttttc
    13561 cgaattgaac gctgtgtggc tgcatggtga tcggctcagt ccggattatg ctcagttttt
    13621 tgtagcatat caacgatttt cgcgggcacc ccaccgatcg aaccggcgcc catgcacaac
    13681 accacgtcgc cagcgcgggc ctgctcggcg accacgcgcg gcagctcggc cacgtcgtcg
    13741 acgaacaccg gctccacccg gccggccacg cgcagcgcgc gcgccagcga acgcccgtcg
    13801 gcggccacga tgggctcctc accggcggcg tagacctcgg tcagcagcac gtggtcggcc
    13861 tggcccagca cggcgacgaa gtcctcgaag caatcgcggg tgcgggtgta gcggtgcggc
    13921 tggaaggcca gcaccagccg ctgccccggg aaggcgccac gcgccgccgc cagggtggcg
    13981 gccatctcga ccgggtggtg accgtagtcg tcgatcaggg tgaaacgtcc gccgtgcggg
    14041 gccggccact cgccgtagcg ctgaaagcgt cggcccacgc ccttgaactc ggccagcgcc
    14101 ttctgcaccg cggcgtcggg caggttcagc tcgaccgcca ccgcgatggc cgccagcgcg
    14161 ttgagcacgt tgtgctcgcc agccaggttg agcaccaccg gcaggtcggg caactgcacg
    14221 ccgttgcgcc gctgcaccgt gaagtgcatc tgcccgccga ccgcgcgcac atccaccgca
    14281 cgcacctggg cgtcggcggc gaagccatag ctggtgaccg ggcagctcag ctggccggcg
    14341 atgtcgcgca ccgcagggct ctccaggcac aggatggcgg tgccgtagaa cggcatgcgg
    14401 tgcaggaagt cgatgaacgc cttcttgagc cggccgaagt cgtgcccgta ggtctccatg
    14461 tggtcggcgt cgatgttggt gacgaccgcc agcaccggca gcaggttcag gaacgaagcg
    14521 tcggattcat cggcctccac cacgatgtgg tccccggagc ccaggcgcgc gttggcgcca
    14581 gcgctgttca ggcgcccgcc gatcacgaag gtggggtcca gcccaccctc ggccagcacg
    14641 ctggcgacca ggctggtggt ggtggtcttg ccgtgcgtgc cggcgatggc gatgccctgc
    14701 ttcaggcgca tcagctcggc cagcatgatg gcgcgcggca ccaccgggat gcggcgctcg
    14761 cgcgccacca gcacctcggg gttgtcgggc ttgacggcgg tggaggtgac gaccgcgtcg
    14821 gccccttcca catgcgcggc ggcgtggccc acgaacgtgc ggatgcccag ctgcgacagg
    14881 cggtgcagcg cggcaccgtc ggccaggtcg gagccggaga tgacgtagcc caggttgtgc
    14941 agcacctcgg caatgccgct catgccggcg ccgccgatgc cgacgaagtg gatgtgacgg
    15001 atggcgtgtc tcatgcggcc agctcctcgc aggccgcgac catctcttcg accgcctgcg
    15061 tcttttggaa ggttttggcc ttcagcgccc gctccatcag cgccgaacgc tccatatttt
    15121 gtagcatctg ggccaacgac tcgggactca gttcgctctg ccgcaccagc cagccgcccc
    15181 cctgatcgac caggaagcgg gcgttggtgg tctgatggtc gtcgaccgcg tgcggaaacg
    15241 gcacgaacag cgccgccgcg ccgatggcgg cgatctcggt cacggtggtg gcaccggcgc
    15301 ggcagatcac cagatcggcc tcggccaggg cctgcgccat gtcgtcgatg aaaggcagca
    15361 gctcggcctg cacgccggcg gtggcgtagt tggcacgcaa tgcgtcgatc tggcgcgcgc
    15421 cgctctggtg acggacctgc gggcgcgcgt cgggcggcag cagggccaat gcgcgcggca
    15481 ccacgtcgtt gagcgcctgc gcgcccaggc tgccgccgac caccagcaca cgcagcggcc
    15541 cgtggcgacc ggcaaagcgc tgcgccggtg cgggctgggc ggcgaactcc gggcgcagcg
    15601 ggttgccgat ccagcgcccc ttgcgcagca cctgcgggaa ggccgtgaag acgcggtcgg
    15661 ccacgcccgc cagtacccgg ttggccatgc cggcgaccga attctgctcg tgcagcacca
    15721 gcggtttgcc cagtgccaca cccatcaggc cgcccgggaa agtgatgtag ccgcccaggc
    15781 ccaccagcac gtcgggccgc acgcgccgga tcacgccgat gctctgcgca aacgcccgca
    15841 gcaggcgcag cggcagcagc gccagcgtga ccaggccctt gccgcgcacg ccgccgaaat
    15901 cgatcgcctc gaacgggtag ccccgcggcg gcaccagttg ctgctccatg ctgcccggcg
    15961 cgcccagcca gtgcacgcgc cagccgcgcg cgcgcagacc gtcggccacc gccagccccg
    16021 ggaagatgtg ccccccggtg ccgccggcca tcaccagcgc acatttctgc ttcatgcgcg
    16081 gcctccatgc atcaggcgac ggttttccac gtccacgcgc agcacgatgg cgatcgccac
    16141 catgttggcc aggatggccg aaccgccgta gctcatgaag ggcagcgtca accccttggt
    16201 cggcaacgca cccaggttga cgccgatgtt gatgaaggcc tggaacccga gccacaggcc
    16261 gatgccctgc gccaccaggc cggagaacac gcggtccagc gcgatcgctt ggcggccgat
    16321 gtgcatgatg cggcgggtca gccagaaaaa cagaaagatc acgcacacca ggccgaccag
    16381 cccgaactcc tcgccaatca ccgccagcag aaagtcggtg tgcgcttcgg gcagccagtg
    16441 cagcttctcg atgctgcccc ccagaccgac accgaagatc tcgccgcgcc cgatggcgat
    16501 cagcgcgtgc gacaactggt agcccttgcc cagtgcatgg tcctcgctga atggatcgag
    16561 gtaggcgaag atgcgctcgc gccgccaggg cgaagcccag atcatgaccg cgaaggccac
    16621 caccaggatg gccgcgatga gaaagaacat gcgggcgttg acgccaccca gaaacaggat
    16681 gcccatggcg atgaccgcga tcaccaggaa ggcgcccatg tcgggctgcg ccagcagcag
    16741 cagcccgacc acgccgaccg ccacgcccat gggcagcacg gcgcggaaga agcgctcctt
    16801 gacgtccatc ttgcgcacca tgtagtcggc cgcatacagc agcacggcca tcttggccag
    16861 ctcggacggc tggaaattga tcggaccgag cgagatccag cgtcgcgcgc cgttcaccac
    16921 cgtgccgaca tgcgggatca gcaccgcgat cagcagcccc agcgcgctga cgaacagcca
    16981 gggcgcgact ttttcccacc agtccatgcg cacctgaaag gcgatcaggg ccgcgacgaa
    17041 ggacagcacg atccagaacg cgtgccgcag cacgaaatgg cctggcgcat accggctgaa
    17101 gcgcgggttg tcgggcaggg cgatgctggc cgaatacacc atcaccagac cccaggccag
    17161 caacgccacg cacacccaga tcagcgactg gtcgaacccc agcacgcgcg tgggcgtact
    17221 ggtggtgcgc gcataaggca tgccgccgat gcgcaccggc agctcgtcca tctggcgcgc
    17281 gcgcgcggcg cgccgcttct tgccgccgtc cgacggcatc ggcgcagtgg cgctcatgcc
    17341 agcacctcca gctccacccc cgccgccagc gcctgctcac gcaccgcggc gcggaaggca
    17401 tcggcgcgcg ccacgtagtt ggcgtacatg tccaggctgg cgcaggccgg cgacagcagc
    17461 accgcatcgc ccgcctgggc gcgcgagcgc gccagggcca cggcggcacc catgtcctcg
    17521 gccgacagca gctccacccc ggcctgctgc agcgccgcgt gcacctgggg cgcgtcgcgc
    17581 ccgatcagca ccgccgcgcg cgcgaagcgc gacaccggcg ccgccagggg agaaaagtcc
    17641 tgccccttgc cgtcgccgcc caaaatcacc accagccggc gctcggcacc caggccttcc
    17701 agcgccgcca ccgtggcccc gacgttggtg cctttgctgt cgtcgaagta ctcgacgccc
    17761 tccagcacac cgaccgactc cacccggtgc ggttcgcccc ggtactcgcg caggccgtac
    17821 agcatggggc cgagcgcgcc gccggcgctg ctggccaggg ccagcgcggc cagtgcgttg
    17881 acggcattgt ggcgaccgcg gatgcgcagc gcgtcggccg gcatcaggcg ctgcaggtgc
    17941 agttcgagct cggcgtcctt gcgccggcgc tgggtctcgt cggcctcctg cgcgcgcacc
    18001 agccaggcca tgcccgacac catctcgatg ccgaagtcgc ccggcgcatc gggcaggtcg
    18061 gcgccgaagg taacgacgcg gcgcggcgtc ggccgcgggc ccttgaacga cctggccggc
    18121 accgccggcc gcatgccgat caccaccgga tcgttgcggt tcagcaccat cacgccgtgc
    18181 tcgccgaaga tgcgcgcctt ggcggcggcg tagctcgcca tgtcgccatg ccagtccaga
    18241 tggtcctgcg tgacgttgag caccgtggcc gcggtgggct cgaagccggc cacgccatcg
    18301 agctggaagc tcgacagctc cagcacccag acctcgggca gcgcctcgcc gtcgagccgc
    18361 gcctgcagcg tgtcgagcag gctgggaccg atgttgccgg ccaccgccac cgacgggccg
    18421 gcgcgctcga ccagctgtcc ggtgagcgcc gtcaccgtgg tcttgccgtt ggtgccggtc
    18481 accgccagca gcctgggctg atagccgcgc gcctgcccaa gctcctgcag cgcctgcatg
    18541 aacagctcca gctcgccgcc gaccggcagg ccccgctcgc gcgccgcctg caccagcgtg
    18601 gcgatgctgg caggtgccag cccgggactg cggtagacgg cgcgcacgct ggcgtcttcg
    18661 agcagcgtgg cgtcgagcgc gccacagatg aagcgggcgg ccggcaggca ctcgcgcagc
    18721 gcggccagct gaggcggctc ggcgcgcgtg tcggccaccg tgaccgaggc gccctgacgg
    18781 gcacaccagc gggccatggc caggcccgac aggcccaggc ccagcaccag cacgtgacga
    18841 tcttgcagca ggttcatcgg ctacctcagc ttgagcgacg acaaacctgc caggcacagc
    18901 agcatggtga tgatccagaa gcgcaccacc accttggtct cggcccagcc gttcttctcg
    18961 aagtggtggt gcagcggcgc catcttgagc aggcgccggc cttcgccgta gcgccgcttg
    19021 gtgtacttga accagcccac ctgcagcatc accgacagcg cctcgacgac gaagatgccg
    19081 cccatgatgg ccagcacgat ctcctgccgc acgatgacgg cgatggtgcc cagcgccccg
    19141 cccagcgcca gcgcgccgac atcgcccatg aagacctccg ccgggtgggc gttgaaccac
    19201 aggaaggcca gtccggcccc ggccagtgcg gcgcagaaga tcagcagttc gcccgtgccc
    19261 ggaatgtgcg ggatcagcag gtagcgcgca tacacggcgt tgccggtgac gtaggcaaac
    19321 acacccagtg ccgaaccgac caggatcacc ggcatgatgg ccaggccatc cagcccgtcg
    19381 gtcaggttga cggcgttgct ggagccgacg atcaccaggt agctcaggat cacgaagccc
    19441 agcaccccca gcgggtagtt gatctccttg aagaacggca ccagcagacc ggcggccggt
    19501 ggcaggtcga tgctgaagcc ggaactcacc cagttgacga acagcgccca gacgcgcgcg
    19561 ttggacgcct cggaaatgct gaacaccagg tagaaggcag cgaccagacc gatcaccgac
    19621 tgccagaagt acttctcgcg cgagcgcatg ccttccgggt ccttgttgac caccttgcgc
    19681 cagtcgtcca cccagccgat ggcaccaaag ccaaaggtga ccagcatcac gatccacacg
    19741 aagcggttgg accagtcgaa ccacagcagc gtggagacgg cgatcgacag caggatcagc
    19801 accccgccca tggtgggcgt gccactcttg accaggtgcg actgcatggc gtagccgcgc
    19861 accggctggc cgatcttcag cgccgtcaga cggcggatca cccaggggcc cagcgcaatg
    19921 ccgatcacca gcgccgtcat ggccgccatc agcgcgcgga aggtgaggta ctggaacacg
    19981 cggaagaagc cgaagtccgg cgacagcgtc tgcaaccagg tggccaggct aggcagcatg
    20041 ggcgtcctcc gcggttgggc cggtggcggc cgacagcgcc tccaccacgc gctccatgcg
    20101 catgaagcgc gaccccttga ccgccagcgt ggcgcactgc ggcaggtggg tgcgcacggc
    20161 gtcgatgagc gcgccgatgt cgtcgaaatg ccgccctccc ccgaaggcca tggcggcgtg
    20221 gatggcctgc ggcccgtgcg ccagcaggtg ctccacgccg cgctggcggg cgtaggcgcc
    20281 gacttcggcg tggaaggccg ggccctgctc gcccacctcg cccatgtcgc ccagcaccag
    20341 caggcgcggg ccgggcagct cggccagcac gtcgatcacg gcgcgcaccg agtccgggtt
    20401 ggcgttgtag gtgtcgtcga ccagggtgat ggcacgacca cgctggcgca gcagcacggt
    20461 gcgcgaacgg cccttgaccg ggcggaactc gcccagaccg cgggcgatgg ccgccagcgg
    20521 tgccccggcg gccagcgcac aggcggtggc tgccagcgcg ttgtgcaggt tgtgacggcc
    20581 cgccacatgc agggcgccgt cgaactggcc gaccggcgtg gcgacgctca ccgcccagtg
    20641 gtcgaccgcc caggtggcct gggacaggtg cacggcagcg ccggcgccaa agtcgagcac
    20701 acgacggcca ccggccagtg agcgccagag cggactgaac gcatcggccg ccgggaacac
    20761 cgccacgcca tcggccggca gcgcggtcag cacggcgccg ttttcgcgcg ccaccgcctc
    20821 cacgctggcc atgaattcct ggtgctcacg ttgggcgttg ttgaccagcg ccacggtggg
    20881 tgcggcgatg gccgccagtt gcgcaatctc gccgggatgg ttcatgccca gctccaccac
    20941 cgccacccgg tgctgcgggc gcaggcgcag cagggtcagc ggcagtccaa tgtcgttgtt
    21001 caggttgcct tcggtcgaca gcgcggctgt cccctgccag gcgcggaaga tcgaggcgac
    21061 catctgcgtc accgtggtct tgccgttgct gccggtgacg gcgatcagcg ggccgtcgaa
    21121 acggcggcgc caccggtgcg ccagctcgcc cagggcaagg cggacgtcgg gcaccagcac
    21181 gcccggcaga ccggcggcca ccagccgggc ctcggcgcgc tcgtcgcaca acgccgccac
    21241 cgccccctgc gccgccgcct cgcccaggaa gctcgcggca tcaaaacgct cgcccttgag
    21301 cgccacgaac aggtcaccgg cctgcaggct gcgggtgtcg gaatgcacgc gcgcgcagcg
    21361 cacggcgccg tcgccgacca ggcgtgcgcc ggtcagccag ccggccagct cctgcaggct
    21421 cagtgccatg gcgctcatgc cggcgcctcc cccgtggcga cgcgccgctg cagcacccgg
    21481 cgcgccacgg cctggtcgga gaacggcgtg cgttgcccgc gcacctcctg atagtcctcg
    21541 tggcccttgc cggcgatcag caccacgtcg gccggctgcg cttcggtcag ggccgccgca
    21601 atggcctggg cgcggtccac ctcgaaccgt gccgcgcccg ggcggcgcag gccggcgccg
    21661 atgacgtcga tgatggcctg cgggtcctcg tggcgcgggt tgtcgctggt caccaccagc
    21721 cgatcggccg ccgcctcggc cgcctgcgcc atcagcgggc gcttgctggc gtcgcggttg
    21781 ccgccgcagc cgaacacgca ccacaggcgc ccaccgcgct gcgccgccag cggtcgcagg
    21841 gcgcgcaggg ccttgtcgat ggcatccggc gtgtgggcgt aatcgaccac cgccagcggc
    21901 gcaccgggca ggccgatgcg ctccatgcgc cccggcaccg caggcaggtc ggtgcaggcg
    21961 gccacggcct gctgcagcgg cacgcccagc gcccgcagcg tggccagcac gcccatcagg
    22021 ttgtcgatgt tgtactcgcc caccaaagtg gtgcgcaggg cggcgcgctg ctcgccctcg
    22081 cacaccaccc agtgcagacc tgcgtcggca taggcgatgt cggtggcgcg caggcgcgcc
    22141 gggcgctccc gcgccacggt ccagacgtcc agcccgcgcg cctgtgcctt ggccaccagt
    22201 tcgccggccc gcgggtcgtc ggcgtggatc accgccgcct gcagacccgg ccagtcgaac
    22261 agcgcagcct tggcctgcca gtaggcctgc atgctgccgt ggtagtcgag gtggtcctgg
    22321 gtgaagttgg tgaacaccgc cacgcggatc gcggtgccgt ccaggcgccg ctccgcaatg
    22381 ccgatggacg aggcctcgat ggcgcaggcg cgcacccccg cgtcgaccag ctcacgaaag
    22441 ttccgctgca gcagcaccgg gtcgggcgtg gtcatgccgg tgacctgcag cgcctgcggc
    22501 agacccacac ccaaagtacc caccagcgcg cacggggaaa gcgtcggaag ctctgttttt
    22561 gatagcgccc agctggtcca ccaggcggtc gaggtcttgc cgttggtgcc ggtcaccgcc
    22621 accacctgga gcgcctgcga cggctcgccg tagtaggccg ctgcgatcgg accggtgtcg
    22681 gccttcaggt gcgcgtagct ggccacctgt ggctgttcga agccgaacgc ctgcaccccc
    22741 tcgtgttcga ccaggcaggc gcgtgcaccc tgtgccagcg ccgcggccac gaaccgccgg
    22801 ccgtccacgg ccgctcccgg ccaggcgatg aagccgtcgc cgggctccac ctggcggctg
    22861 tcggcgtgca gccgacccgt cacgcgggca cgcagccagc gggcggcttc ggtcgggtgg
    22921 tggcactggg caagggccat gtcagaacga ctcctcgacc tgctcggcca cgatggcggg
    22981 cttgaccgcc atgtcgggct gcacgcccat gatgcgcagc gtctgctgca ccacttcgct
    23041 gaacaccggc gccgccacgg cgccgccgta gtacacgccg ttgctgggct cgtcgatcat
    23101 cacggcgacg ataacgcgcg gctgctcgat cggcgccatg ccggtgaacc aggcgcggta
    23161 ccggttgctg gcataaccct tgccgacctg cttgcgcgcc gtgcccgact tgccgcctac
    23221 cgaatagccg acggtctgcg cccgttggcc agtgccaccg gggccggcgg ccagtgccaa
    23281 catcttgcgc acggcgcgcg cgttcttttc ggaaaacacg cgcaccccct ccacgctggc
    23341 gccttcgggc gctttcagca gcgtggccgg aatcacccgc ccgtcgtgcg agaacgcggt
    23401 gtaggaatgc gccatctgga acagcgacgc cgacaggccg tagccatagg acatggtggc
    23461 ctgttccacc gggcgccagc tcttccagtg acgcacccgg cctgccgccg cgccgggaaa
    23521 ggccagctcc ggcttgcggc cataaccgag cgcggtgtag gtttcccaca tttcctgcgc
    23581 gctcaggcgc tgggcgatct tcagcgagcc gacgttgctg gacttctgca ccacgccctc
    23641 gacggtcagg gtgccgtagt tgtgggtgtc gcggatggtg aacttgtcca gcttgtagct
    23701 gcccggtgcg gtggcgatca acgtctgcgg cgtcaccttg ccggcctcca gcgccatggc
    23761 cacggtgacc ggcttcatgg tcgagccggg ctcgaaggtg tcggtgacgg cgatgttgcg
    23821 cagctgcgcc ccgctcaggt ggcgacggtt gtcgggcgaa tagctggggt agttggccag
    23881 cgccagcact tcgccggtct gggcgtcgag caccaccacg ctgcccgacc tggccttgtt
    23941 cgatgtgacg gcgtcgcgca gcttctgata ggcgaagaac tgcaccttgg agtcgatcga
    24001 cagctgcacg tcctgaccgt ccaccggcgg gacttcctcg cccaccgctt cgaccacgcg
    24061 tcccagccgg tccttgatga cgcggcgcga gccggcgcgc ccggccaggc gcttttcgaa
    24121 cgacagctcg gcgccctcct gcccctggtt ctccacgttg gcaaaaccca ccacgtgggc
    24181 ggcggcttcg ccctccgggt acttgcgctt gtattccttg cgctgataga tgccggccag
    24241 cttgagctcg gcgatctgcc gcgccaccga ctcgtccacc tggcgcttga gccagacgaa
    24301 ggtcttgtcc tcatcggaca gcttcttgtt cagctcagcc agcggcatgc tcaacaggcg
    24361 cgccagctcg cgcagcctgg ccgggtcgcg atccacgtcc tccggaatcg cccagatgct
    24421 gggcgccacc acgctggacg ccagcagcag gccgttgcga tcgaggatgc gtccgcggct
    24481 ggccggcagc tccagggtgc gggcaaagcg cacctgaccc tggcgctgaa agaaggcgtt
    24541 gccgaacacc tgcacatagg cggcgcgccc gatcagcacc aggaaggcca gcgccagcat
    24601 ggcgacgatg aacttgctgc gccacaccgg cgtcttggac gccagcagcg ggctggaggt
    24661 gtagcggatg tccctcatgg ccgctcgcct tgcggcgccg acgccgccgg cagcgccagc
    24721 gacgaggtca cgtattcggt gatggccggc gtgatgctgc gcatggccag cttgtcgcgc
    24781 gccagcttct cgacgcgcag cgaggtggcc tcggcgcgct tttccacctc cagtcgggcg
    24841 cgctcggtct ccagacgatg cgcctcggcg ctggcccggt cgagctcggt gaacaggcgg
    24901 cgcgagtcgt actgcacgcg caccacgtac agcgccgaca ccatgacggc caccagcagc
    24961 acgatggaca ggcgcgtcat gcccggcccc cgtgatgcgt gggcgccgcg gtgcgctcgg
    25021 ccacccgcat gatggcgctg cgggcgcgcg ggttggcggc cagttcggcc gcaccggggc
    25081 gcgatttgcc cagcgcgcgc aaccgtgggg gcgccggttc ggcaaacggc gcgcggcgat
    25141 ccaccggcgc gcgcgcgtgg cgtgcgatga actgcttgac gatgcggtct tccagcgagt
    25201 ggaagctgat caccaccagc cggcccccgg gttgcagcac ctgcacagcg gcctctagcg
    25261 cctgttcgag ctcttcaagc tcggcgttga cgaaaatccg aacagcctga aatgtgcgcg
    25321 ttgcagggtt ctggcccggc tcgcgggttt tgaccgcgcc agccacgagc tcggccagct
    25381 cgagggtgga tgcaacagcg cgccccgcct gtcggcgagc gacaatcgcc tttgcaatgg
    25441 cgacagcaaa ccgttcctcg ccgtagtcac ggatcacctg cgcaatctgc cgttcgtcgg
    25501 catgggccag ccagtcggcc gcgctctggc cgcgcgtggg atccatgcgc atgtccagcg
    25561 gcccgtcggc acgaaaagaa aaaccccgcg cggggttgtc gatctgcggt gagctgatgc
    25621 ccagatccat caacaccccc gccacgctgt gcggtggcca ctcgcccagc gcagcaaacc
    25681 cctgatggcg gatggcaaaa cgcgcgtccc ggatgtgcgc cgcctcggcc agcgcctcgg
    25741 ggtccttgtc gaaggccgtc aaccggccct cgggcgccag ccgccgcagg atctgccgcg
    25801 catgcccgcc gcggccgaag gtggcgtcca cgtagcggcc gcgcgggtcc gtgaccaggg
    25861 catcgaccgc ctcgttcaac aggaccgtgg tgtgtgacca tgcgctcact cgcccccgcc
    25921 cctagaaggt gaagtcctcg aacgagccgg gcagcccggc ctgcatggcc tgggcttcct
    25981 tggcttcgta ggtgagcttg tcccacagct cgaaatgccg ccccatgccc agcagcatca
    26041 cctcgcgcgt caaccctgcg gcctggcgca gttcgggcga caccagcacg cggcccgtgc
    26101 cgtcgagctc cacatccatc gcactgccca gaaagatgcg cttgacccac tgcgccgaca
    26161 tgggcagctg gatcaggcgt tcacggaact tctcccattc ggggcgcgga aacaccatca
    26221 gacagccgtc cgggtgcttg gtgatggtga gctgaccact ggcggtcgcc gccaggacgt
    26281 cacgatgccg ggtcggcacc gacagcctcc cctttgcatc cagactcaac gatgacgccc
    26341 cttgaaacac gctctatccc cgtggcaaag gcagcaaccg ggaagaaatg gcactttccc
    26401 ccacttaact gcacttattt gcactgtagc aggaatcggc cgcgccacaa caggggtcaa
    26461 caaattattt caatgaaatc aacgcgttag ccaacttctt cactggttat ccaaggcgaa
    26521 aactctttca aatgagcgac ttagatcgga tagtgaaagt cagatcgatc caggttgtgg
    26581 ataacctgcc ctggcgcgcg ggacaagccg ggactttgcc ctggcgatcg ggcgccatgc
    26641 cgtcaggcat gcggcggcgc ctcataagct atatttttta tagctcttgg cgcttgctgg
    26701 gagctcgctc a
//
LOCUS       DAOOOG010000014        26420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_1684_length_26420_cov_18.175232, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000014 DAOOOG010000000
VERSION     DAOOOG010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 26420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26420
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1684_length_26420_cov_18.175232"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="PK242_02325"
     CDS             <1..114
                     /locus_tag="PK242_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynurenine formamidase"
                     /protein_id="HOZ92890.1"
                     /translation="ENLVLDAVPEGDYELIALPLKLSTADASPVRAVLRAL"
     gene            131..910
                     /locus_tag="PK242_02330"
                     /pseudo
     CDS             131..910
                     /locus_tag="PK242_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407237.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            912..1712
                     /locus_tag="PK242_02335"
                     /pseudo
     CDS             912..1712
                     /locus_tag="PK242_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1648..2811
                     /locus_tag="PK242_02340"
     CDS             1648..2811
                     /locus_tag="PK242_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOZ92891.1"
                     /translation="MRCWPHCKAHNSPLPPGFHRPDPASALQKSLPMTSSLLQRLGIA
                     LPIIQGPMTGSDTPALAAAVSAAGGLGMLGCGMRSPAAMAEAAAAVRQQTDRPFGMNL
                     FVQATPNPDEATVQAAMERLAPLYAELGLQPQRPTQWCEDFAAQFEALVALRPAVASF
                     TFGILDAAQVARLHAAGCLVVGTATTVAEARAWAQVGADVVCASGTEAGGHRGTFLGD
                     FTASQVGTLALVPQCVDAVDIPVIAAGGIMDGRGIAAAQALGAQAVQMGTAFLACPES
                     GIGPAYRQALAQAQDTDTRLTRVFSGRPARGIVNAMMEALAAEEDRVPAYPVQNALTG
                     ALRRAAAAQGRTSHLSLWAGQGVGAVRALPAGELVAVLADEWRAACGRLAAAA"
     gene            3650..3922
                     /locus_tag="PK242_02345"
                     /pseudo
     CDS             3650..3922
                     /locus_tag="PK242_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            3978..4256
                     /locus_tag="PK242_02350"
     CDS             3978..4256
                     /locus_tag="PK242_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOZ92892.1"
                     /translation="MNVTFDPAKDAANVAKHGVSLTEAAGFEWDTAIVWPDTRNDYGE
                     ARMVALGYIGLRIMAVVFVDRPADKPNERRVISLRKANSREVKRYAET"
     gene            4243..4524
                     /locus_tag="PK242_02355"
     CDS             4243..4524
                     /locus_tag="PK242_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOZ92893.1"
                     /translation="MPKLKAGTILPTPAEDAAITAAALADTDAQPFTDAEWEQVKPLA
                     RRGRPLGSGSKAQVTLRLDVEVLEKFKASGDGWQTRINEALKSWVRTHA"
     gene            4674..5567
                     /locus_tag="PK242_02360"
     CDS             4674..5567
                     /locus_tag="PK242_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92894.1"
                     /translation="MKFAYPEYGVHELKPYEIKENGVYITSAFRDLCPPGTVFNWTPA
                     DEMGWPGSPNPDTAPFLPIPFDAAQLAAAMIDGAGRSIAYSMEHRLGYPLDDDALNRF
                     SDRMRWMHDALAEAYQLAAAAQLVVGEFDHQAEASAYALAGQYEDAADAANEREGVFA
                     PDVSEDERRTRRDRAVASVLPLQVALDSAMDASAKEWRQWRTAMVRQLLNPATPEAAA
                     AQLKANPPEPLMAQSASTAPGNGKVWTPERLADAKAYREKHGTKKTAEHFGVSEQLIR
                     QKLPSEKAIPKGYSAFTHRPK"
     gene            5721..5930
                     /locus_tag="PK242_02365"
     CDS             5721..5930
                     /locus_tag="PK242_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOZ92895.1"
                     /translation="MAAKTIQLTPLALETRTALPTPEAAGHLNRAQQTLRIWACREDG
                     PIRPIRINGRLAWPVAELRRILGVA"
     gene            5930..6115
                     /locus_tag="PK242_02370"
     CDS             5930..6115
                     /locus_tag="PK242_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92896.1"
                     /translation="MEALYQHPDGMGAIAFDAATSRLFLLNEAEALSAWAYIGPTGLR
                     VLACCLLAAADEVEAKQ"
     gene            6271..6474
                     /locus_tag="PK242_02375"
     CDS             6271..6474
                     /locus_tag="PK242_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92897.1"
                     /translation="MNHPEFEARTQSWRLGAVVFQLRTLGWPVETIEIPSPTDECPHR
                     IIARYHLAPHHVAQALAQLGAAR"
     gene            6471..8660
                     /locus_tag="PK242_02380"
     CDS             6471..8660
                     /locus_tag="PK242_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ92898.1"
                     /translation="MMADTERARAALQSIPPDCDRDTWVRAGMAAHAAGLDFEDFDTW
                     SAQAGNYDARAARDTWRSFKAGKGVGAGTLHRMAGEHGHQQERPSKPPKRAATPPPAP
                     RPGMAPADVWARCEPATWAHPYIERKAAAGVPLDALRVVPQGDALRIGGHPMAGALAV
                     PAYAADGRLQSLQLIPPKGEKMNLAGAPMAGASFTVGTPGDGPLYIVEGIGQAWACWQ
                     ATGRAAVCCFGWGNVGTVAKQLQGRALVLVPDVGKEAQAEAIARELGCSVAAMPQGEA
                     DNFDCNDLAQRDGADVLAELLESAQSFKPEPLLTPVDVAGVLTHPSPPPRFVWNGYCP
                     RGVVTLLGAHGGTGKSTIALMLAAAVATGRELFGIATDAAPVVFASFEDGAHIVRHRL
                     AHICQCWGLEPAELAGLHIVDGTANPELFACEGRGPGDVTPAYAELLALVKQTGAGLV
                     IVDNASDAYGGDEIQRRQVRAFMRALAVIAREGDAAVLLLAHVDKGTSRRERSDTEGY
                     SGSTAWHNSARSRIFMSRDDGVLTLAHQKNQFGRMREPLALQWPEGGLPQAASSNPFS
                     ERLQGRADDERAAALLRLIVEFEGREQYCSPALFARNNVHALLKSEPAFLALKLRADD
                     TRRIVNQCQRAGWLEVLDYRSPDRKERQRWAVTQTGRDFINLPAPTAPTAPTYEDGAQ
                     GAGGAPTAPTYVGGMGDSARTNEGASAGAEAGLTAPTCTTTEGHTHD"
     gene            8653..9039
                     /locus_tag="PK242_02385"
     CDS             8653..9039
                     /locus_tag="PK242_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOZ92899.1"
                     /translation="MIDCLIQGKLFAAPQQRTSKAGKPFATAKLIAAAGDGERLFVNV
                     IAFDAAAVAALLALGAGDALALAGTATPRVYIKDGEPRASLDVTAQQVLTAHHVARKR
                     SAMQAPDRAAPERDRRFDAWEAARAD"
     gene            9029..9217
                     /locus_tag="PK242_02390"
     CDS             9029..9217
                     /locus_tag="PK242_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92900.1"
                     /translation="MLTDYIAHALRCPRCTAAARLPGARRCRPGAELWRNYLAALPPP
                     VQYDSDKRRAARRTAQGP"
     gene            9269..9526
                     /locus_tag="PK242_02395"
     CDS             9269..9526
                     /locus_tag="PK242_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92901.1"
                     /translation="METQYDKLMRPRAIASPPARGFNGPLAAKIAQHQAIAAALAQCP
                     GASLATLAVSEHERNAIRNRAGRIAQARREQAARPGAPARR"
     gene            9551..10243
                     /locus_tag="PK242_02400"
     CDS             9551..10243
                     /locus_tag="PK242_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92902.1"
                     /translation="MSEKLKPLSEHPDYHNAAERLAHFHRELAAAQAEAARIDVERLA
                     APGQRPADDPLARADALLSGAEPTPALSLRAGKNQELIAALRKAIAAQAIVLRDIARA
                     HAADVREQSTAEHIKLAQAVLNAADALVQANEAEAGFRQELAALGYDGAVPGMSYAPP
                     PERAVDLTEGYGLAWRTEVSDYCRTGRTAAELDGAKRDADAAVQAAATAAQAARPKKA
                     PAVDASKGELVN"
     gene            10487..10615
                     /locus_tag="PK242_02405"
     CDS             10487..10615
                     /locus_tag="PK242_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92903.1"
                     /translation="MQLPERADPLQWLLAAMNCPALPLRLRADVAAFLLPYCRARK"
     gene            complement(10864..10939)
                     /locus_tag="PK242_02410"
     tRNA            complement(10864..10939)
                     /locus_tag="PK242_02410"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10904..10906),aa:Lys,seq:ttt)
     gene            complement(10964..11776)
                     /locus_tag="PK242_02415"
     CDS             complement(10964..11776)
                     /locus_tag="PK242_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOZ92904.1"
                     /translation="MSAAAVEADLERRFGGLARLFGAEGAARIRAAHVAVVGIGGVGS
                     WAAEGLARSGVGQITLIDLDHVAESNVNRQIHALTPTFGQAKVQAMRERIGQIHPACV
                     VHAVEDFVAPTNWPQLLPAPVHAVIDACDQVSAKLAMAAWARERGLCFVTVGAAGGKR
                     HGERVEVADLAEVTHDPLLAQLRYRLRRQHGAPTGGKRLGVTAVFSRESVRLPEACEA
                     PQQVDSSLNCHGYGSSVAVTATFGLCAAGWVLDRLSQEGQKNQAIICGLPSD"
     gene            complement(11773..12891)
                     /locus_tag="PK242_02420"
     CDS             complement(11773..12891)
                     /locus_tag="PK242_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HOZ92905.1"
                     /translation="MPQPPDLDLLLRAVLLGTFALSAVFGAIMHRTGFCTMGAISDVV
                     NMGETTRLRQWALAAGVATLGFGVLAAGGWVQPADTVYAGPRWLWLSGLVGGLLFGVG
                     MVLASGCGARMLVRLGGGNLKGLIVFLVMGLASYATLRGITAVLRDRTVDQVEWVFNV
                     PATLGHLAASAGGWPLPVGLLGAALLVGGLLMAWALTGRGFINGNNLLAGLGLGGVIV
                     ALWWLSGHLGYLPEHPDTLEPAYLGTRSGRMEALTFTGPLAHTIDWFIFFSDRSQRLS
                     LGVVAVFGLVAGAALHALASRRFRWEGFRTTEDLANHLIGGTLMGVGGVTALGCTIGQ
                     GLSGISNLSLTSFTALAGIVGGAVLALKYQGWRLERSL"
     gene            13318..16206
                     /locus_tag="PK242_02425"
     CDS             13318..16206
                     /locus_tag="PK242_02425"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOZ92906.1"
                     /translation="MSESLSQPGLASLSKSFEPAAIEARWGPAWEQAGLGRAGYRGSG
                     QPDAGAAARGENFAIQLPPPNVTGTLHMGHAFNQTIMDSLTRYHRMRGFNTAWVPGTD
                     HAGIATQIVVERQLQEQGQSRHDLGRKNFVARVWDWKQQSGDTITRQMRRMGASVDWS
                     REYFTMDDRLSKVVTETFVQLYEQGLIYRGKRLVNWDPELKTAVSDLEVESEEEDGFL
                     WHIAYPLADGSGERLVVATTRPETMLGDVAVMVHPEDERYQRFIGKAVTLPLVGRAIP
                     VIADDYVDKDFGTGVVKVTPAHDANDYAVGQRHGLPMIGVLNLDATINDNAPAIFRGL
                     NRFAARKAIVADLEAQGLLVETRKHKLMVPRCARTGQIIEPMLTDQWFVAMNKAPTLP
                     AAHGARPGAGVATPPDARSIAQKAIDAVQSGQVRFVPEQWVNTYNQWMNNIQDWCISR
                     QLWWGHQIPAWYDEEGNVYVARNEAEAQAQAPGKTLRRDEDVLDTWYSSALVPFSTLG
                     WPEPTEDLNLYLPSSVLVTGHDIIFFWVARMIMMTTHFTGQVPFKDVYIHGLVLDAHG
                     KKMSKSEGNVLDPVDLIDGIALEPLLAKRTTGLRKPETAPKVRENTKKEFPDGIPGYG
                     ADALRFTFAAMATLGRTVNFDSKRCEGYRNFCNKLWNASRFVLMNCEGADCGVEGDTA
                     IERSAADRWIVSLLQQTEAAVAQGFADYRLDQVAQTIYDFVWNEYCDWYLEIAKVQIQ
                     TGNAAQQRATRRTLIRVLEAILRLAHPVIPFVTEALWQVVAPIAGKAGDSIATQPYPQ
                     AQPVHIDEAAQRQIGHLKALVDACRNLRGEMGVSPAQRLPLYALGEADFIRDQAPVLQ
                     ALAKLSEVKVFEHEADWAAAAQAAPVAVVGQARLALFMAVDVAAEKARLGKEAGRLEG
                     EIAKAQGKLNNPAFAAKAPPAVIEQENRRVAEFGAALAKIRDQLQRLG"
     gene            complement(16392..17357)
                     /locus_tag="PK242_02430"
     CDS             complement(16392..17357)
                     /locus_tag="PK242_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ92907.1"
                     /translation="MDILLLEELVADARQWLEARHHVDYRPELLEDEALLRGRLYKVD
                     ALVVPPRLKINAQLLDYAPRLAAVGRIHDGTENLDFEACQKRHVRVIQASSATVRASA
                     EHQLCTLMQLFRSGGQVGPSLSLRRGAPGREINDSVIGLFGLAPPAHVLATMLVPLGA
                     RVVGYDPAVHRNAELWRRLGVQPMALADMLEVADAVSMQVIYASRYRGLVNDRVLAAC
                     KPGQLWTSISRPSLFDLDALAGALRSGRIGALMMDSDDERLTAPDSPLRHAPNVRITP
                     RLAPYTQESHLRGSWYLVDRIHEALMLNETRSQRGTLDSEPVPLD"
     gene            17464..18351
                     /gene="galU"
                     /locus_tag="PK242_02435"
     CDS             17464..18351
                     /gene="galU"
                     /locus_tag="PK242_02435"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOZ92908.1"
                     /translation="MTASRQVLTAVFPVAGLGTRFLPATKASPKEMLPIVDKPLIQYA
                     VEEAYAAGVRHMVFVTGRSKRAIEDHFDTAYELESELEAGGKADLLAMVRAVQPDDMD
                     CTYVRQPRSLGLGHAVLCAAHIVGEQPFAVLLADDLMVGPADGQPVLAQMADAFESLG
                     RSVLAVQQVPADQVRRYGIVAGTPAGERLISIERIVEKPAPEQAPSRMGVAGRYILTP
                     RIFDEIRQQPPGAGGEIQLTDAIARLMHKERVYAYQYSGKRYDCGSKEGFLEATVELA
                     LQHPQMGAPFRDYLKTLSL"
     gene            complement(18476..18703)
                     /locus_tag="PK242_02440"
     CDS             complement(18476..18703)
                     /locus_tag="PK242_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOZ92909.1"
                     /translation="MDIAKEVDARGLNCPLPILKAKKALAELQSGQLLRVLSTDGGAL
                     RDFQAFARQTGNELVEQSTEGEVTAHVLRRR"
     gene            complement(18718..19266)
                     /locus_tag="PK242_02445"
     CDS             complement(18718..19266)
                     /locus_tag="PK242_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOZ92910.1"
                     /translation="MSPFRYCPCCATPLAMIVQEEDSGPRERLRCPACDYTHWNNPVP
                     VLAAIIEYRGQVLLARNAAWPSKMFALITGFMEAGETPQDGIVREIKEETQLDTRSLA
                     LVGVYDFQRMNQVIIAFHAVCEGEVRLSPELADWRLYDPPQLKCWPAGTGYALADWLR
                     ARGHEPVFFTPEENAERRRGLD"
     gene            complement(19337..20239)
                     /gene="cysM"
                     /locus_tag="PK242_02450"
     CDS             complement(19337..20239)
                     /gene="cysM"
                     /locus_tag="PK242_02450"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HOZ92911.1"
                     /translation="MDYPTIEDAIGRTPLVRLQRLGAQEHAARGSVVLAKLEGNNPAG
                     SVKDRPALSMIRRAEERGEIRPGDTLIEATSGNTGIALALAAAIRGYRMVLIMPEDLS
                     VERAQTMKAFGAELILTPRSGGMEYARDLADKMVAQGKGRVLDQFANPDNPRIHYETT
                     GPELWQQTGGRITHFVSAMGTTGTITGVSRFLKEQNPAVRIIGAQPAEGSRIPGIRKW
                     PEEYLPRIYDPAAVDELTLVSQADAEDMARRLAREEGIFGGISSGGAAWVAQQVAARE
                     QGATVVFVVCDRGDRYLSTGVFPA"
     gene            20281..20367
                     /locus_tag="PK242_02455"
     tRNA            20281..20367
                     /locus_tag="PK242_02455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20315..20317,aa:Leu,seq:caa)
     gene            complement(20416..21681)
                     /locus_tag="PK242_02460"
     CDS             complement(20416..21681)
                     /locus_tag="PK242_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ92912.1"
                     /translation="MDFAYSTRVQALRQQVQRFMDDLILPSHRDWLRWSEAGAYPLDV
                     IEPLKARARAAGLWNLFLPGLRDDEPGTRLSNLEYAPLAEIMGRVPWASEVFNCSAPD
                     TGNIELLQLFATPAQRAHWLRPLMEGTMRSCFAMSEPDVASSDPTNLGTRIARDGDHW
                     VLNGRKWFITGAAHPQCRLAIVMGVSDAAPDVPAHRRHSLLLVPMDAPGLEIVRNIPV
                     MQHQAIEGHCELVFRDVRVPADHLLGTAGEGFAMAQARLGPGRVHHCMRTIGQCELAL
                     ELMAERALERMAFGRHLSDFANVQDWIACSRLEIDQARLLVLRAAWRMDTEGNAAART
                     DVSAIKVVAAQLQTRVLDRAMQVFGAVGLTPDTPLAALWSWGRALRFLDGPDEVHLRT
                     VAREELRRSRAHRGDNVLHLQRWMPPESL"
     gene            complement(21794..22714)
                     /locus_tag="PK242_02465"
     CDS             complement(21794..22714)
                     /locus_tag="PK242_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOZ92913.1"
                     /translation="MPSNQNPYGALPARFRRPRVLIVGCGDVGSRAARALPPRVRVLA
                     LARSAEAAAALRQQAIVPLAGDLDQAASLDRLAGIATHVLHLAPPPAVGEGDPRTAAL
                     LRALARRRAPLALVYGSTSGVYGDCGGAWVDETRPVRPQTARARRRVAAETWVRAFGR
                     AAGVRASILRIPGIYAPDRPGGTPRERLQRGTPVLAPADDVFTNHIHADDLARAFVLA
                     LWRGRPQRVVHACDDSQLKMGDYVDLAADLYGLPRPPRIDRQAADAQLPATLLSFMSE
                     SRRMHNTRLKRELRLRLRHPDVHTGLLPKT"
     gene            22764..23807
                     /locus_tag="PK242_02470"
     CDS             22764..23807
                     /locus_tag="PK242_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HOZ92914.1"
                     /translation="MTHTITVEPSGRSFQAEAGEPMLTAGIRQGIGLPYGCKDGACGS
                     CKVKKLSGQVTLGPHQDKALSAQEEADGYVLTCCGVATSDVVLESRQVTHAGAFPIKK
                     MPARVALLERLSHDVMRVRLQLPANDIIQYHAGQYVEFILRDGSRRSYSMANAPHTLI
                     ESGSPMVDLHIRHMPGGKFTDHVFGAMKEKEILRIEGPYGSFHLRDESPKPIVLLASG
                     TGFAPIKAIIEHMQFMGITREAVLYWGGRRPQDLYLDAWVRAKTQEMPHLRYVPVVSD
                     ALPEDGWGGRTGFVHRAVLQDLADLSGHQVYACGAPIVVDSARRDYVEQAGLPAEEFY
                     ADAFTSEADKHAG"
     gene            complement(23878..24204)
                     /locus_tag="PK242_02475"
     CDS             complement(23878..24204)
                     /locus_tag="PK242_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92915.1"
                     /translation="MINRWLSVAVTTACMGVGVAHAQGVATPGVDQRQINQERRIEQG
                     VASGQLTPREAARMQRQQNRVAAAEADAKADGRVTATERARLHAMQDRNSRRIHRQKH
                     DRQRVR"
     gene            24355..25335
                     /locus_tag="PK242_02480"
     CDS             24355..25335
                     /locus_tag="PK242_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ92916.1"
                     /translation="MNRRSLLPVLSVALLASAIGAAPAAWAQGKPIRLIVPYAPGGPL
                     DVTARALAERVQPALGNVVVENKPGAGGNIGSDLVAKAAPDGSTIGIAAVATHAINPW
                     LFSKMPYDAGKDFAPITQMVRVPNVLVINADTAQRLGIRTLADLIAYARKNPGKLNYG
                     SGGNGSAGHLAGEMFKQGAGIFAVHIPYNGANPAQLALLAGQVDYNIDNLAAAAANIK
                     AGKIKALAVTSARPSPLLPGVPAMAETIKGFEVDTWWGLVAPAGTPREVIDRLNAAFT
                     DALKAPETQQRFAQLMAEPVPTTPEQFGQLMTRERARYQPVVKRSGAKVD"
     gene            complement(25422..26138)
                     /locus_tag="PK242_02485"
     CDS             complement(25422..26138)
                     /locus_tag="PK242_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ92917.1"
                     /translation="MAQTLLKVSGLKVAYGGIQAVKGVDFEVREGELVSLIGSNGAGK
                     TTTMKAITGSLPLAGGDIEYLGKSIKGQGPWDLVRQGLAMVPEGRGVFTRMSITENLL
                     MGAYIRSDKAGVAQDIERVFSIFPRLRERKDQLAGTMSGGEQQMLAMGRALMCRPKVL
                     LLDEPSMGLSPIMVDKIFEVVQDVYAQGVTVLLVEQNASRALALANRGYVMESGLVSM
                     SGSGKDLLADPKVRAAYLGE"
     gene            complement(26140..>26420)
                     /locus_tag="PK242_02490"
     CDS             complement(26140..>26420)
                     /locus_tag="PK242_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ92918.1"
                     /translation="ARALATDPQLVALDEPAAGMNATEKVQLRALIDRIRKDGRTILL
                     IEHDVKLVMGLCDRVTVLDYGKQIAEGTPAEVQKNEKVIEAYLGTGGH"
BASE COUNT         4415 a         9326 c         8736 g         3943 t
ORIGIN      
        1 gaaaacctgg tgctcgacgc cgtgcccgag ggcgactacg aactcatcgc cctgccgctc
       61 aagctgagca ccgccgatgc ctcgcccgtg cgcgccgtgc tgcgcgccct gtgacccctg
      121 ccccaccgcc atgcaagccc tgctcgacgc cattgccgcc acccctttgc ccaccgaggc
      181 ctgccgcatc ttccatggcc gtggcggcct gcaccccggc tgcgaaggct ggacgctgga
      241 cgcctacccg cccgtgtggg tgttcaccag ctttgaaccc gccgacgacg ccctgctggc
      301 cgccgtgcac caggccctgc aagcgcgctg ggccacactg gcacccggcg ccgcactgca
      361 ctgggtgtac cagtgccgcc acccgcagca acgcgaaacc cgcaccatgg caggccaggt
      421 gcccgaaccg catgtggtgc aggaagacgg cgcacgctac ctggtgcatg tgctgcgcgg
      481 gcagaaccac ggcctgttcc tggacatggc cgaaggccgc cgctgggtgc agcgccacgc
      541 cgcgcagcac cccggcctgc gcgtgctcaa cctgtttgcc tacacctgcg cgttttcggt
      601 ggcggcgctg cgcgcaggcg cggcccaggt ggtcaacgtg gacatggccg ggggcgcact
      661 ggccacgggg cggcagaacc accggctcaa tgggctggag gccggcgccc tgttctggga
      721 ccacgacatc ttcagctcct ggggcaagat cacgcgccac ggcccctacg gcctggtcat
      781 catcgacccg cccagccacc agaaaggcag cttcgtcgcc accaaggact acgcgcgcct
      841 gatgcgccgc ctgcccgacc tgctggcccc cggcggccac gccctgctgt gcctcaacgc
      901 ccccgaactg gaacgtgacc acgcggattc agcagctgga agagcagctg ggcacgccgc
      961 tgttcttgcg ccaagggcgg cgcatggtgc tcacaccggc cggcgaatcc ctgcgcggct
     1021 acgccgaccg cctgctggcc ctggccgaag aggcccgcca ggccgtgcgc ccgcagcagc
     1081 cgggtgggcg cctgcgcctg ggcgccatgg aaagcacggc cgctgcgcgc ctgccccagc
     1141 cgctggcgca actgcatgcc cggtggcccg acgtggtgct ggacttgtcc accggaccct
     1201 cccgacaact ggtggagcaa gtggccgccc acacgctcga tgccgccctg gtcgcctggc
     1261 caccacctgg gctggaaacc gatacgccgg tggaacgcac cccggtgttt caagaatcgc
     1321 tgctgctggc cctgcccgcg caccacccac cggtgcaaag ccccgccgat ttgcaattgc
     1381 acacgctggc cgccttcaca cgcggctgta cctaccgcca catcggagaa gcatggatgc
     1441 aaggcgcccg cggcacagcg ccccaggtgc tggagctggc gtcttaccac gccatcatcg
     1501 cctgcgtggc ggcggggcgc tgcgcgggtg tggttcccca ggccgtatgg gacttgatgc
     1561 gcgagccccc ggccctgcgc ctggtacccc tgcagacctg cgacacggtg ctggtgcgcc
     1621 ggcgcggcta ccagtcgccc gcgctggatg cgctgctggc cgcactgcaa ggcacacaac
     1681 agccctcttc cgcctggatt tcatcgccct gatcccgcct ctgcccttca aaagagcctg
     1741 cccatgacct cttcacttct ccagcgcctt ggcattgcat tgcccatcat ccagggcccg
     1801 atgacgggct cggacacccc cgccctggct gctgccgtgt cggccgcagg tgggctgggc
     1861 atgctgggct gcggcatgcg ctcgcccgcc gccatggccg aggccgctgc cgccgtgcgc
     1921 cagcaaaccg accggccctt tggcatgaac ctgtttgtgc aagccacgcc caaccccgac
     1981 gaggccaccg tgcaagcggc gatggaacgc ctggcgccgc tgtacgccga gctgggcctg
     2041 cagccccagc gccccacgca atggtgcgaa gactttgccg cccagttcga ggcgctggtg
     2101 gccctgcgcc cggcagtggc aagcttcacc ttcggcatct tggacgctgc ccaggtggcc
     2161 cgcctgcacg cggccggctg cttggtcgtg ggcacggcca ccaccgtggc cgaagcgcgc
     2221 gcctgggccc aagtgggggc cgatgtcgtg tgcgccagcg gcaccgaagc tgggggccac
     2281 cggggcacgt tcctgggcga tttcacggca agccaggtag gcaccctggc gctggtgccg
     2341 cagtgcgtgg acgccgtaga catcccggtg attgccgcgg gcggcatcat ggacggccgg
     2401 ggcattgcag ctgcacaggc gctgggcgcg caggccgtgc agatgggcac ggcctttctg
     2461 gcgtgcccgg aatccggcat cggtcccgcg taccggcagg ccctggccca ggcccaagac
     2521 accgacacgc ggctcacccg cgttttctcg ggccgcccgg cgcgcggcat tgtcaacgcg
     2581 atgatggagg ccctggccgc cgaggaagac cgcgtgccag cctaccccgt gcaaaacgcg
     2641 ctgacaggcg ccctccgccg cgcggcggcc gcgcaggggc gcaccagcca cctatcgctg
     2701 tgggcagggc aaggggtggg ggccgtgcgg gccctgccag caggtgagct ggtggcggtg
     2761 ctggccgatg agtggcgtgc agcctgcggg cgcctggccg cggcggcgta accgtcggac
     2821 taggaggggc ggctccctac aatcgggccg caccgccagc cctccatgcc ccacatcatt
     2881 gccaacccct tcacgctgac agagttcctg ctcgacagcc tgccccccga agagcgcgcg
     2941 ggcgtcctgg tccaccccaa acgcatcaaa ggggtcgggt actcgctgct gaaggcgctg
     3001 gacgcctggt gcccgtttcg cctgccgggg tttcaaccct tccctgccgc atacctgcgg
     3061 gcactggaag ccattccggc gcaggcgccg gtgctgatct tcggcatcga gaacatcaag
     3121 gacctgcgca tcctgcgcaa atacttgcgt acgcggcgca ttgcgctgtt cacctggaac
     3181 ccggtgattg actaccagca gaaccaccag gtgcggcagc tgcacatgca gcagctcaag
     3241 gggctggggt tccacatttt tacgttcgac cccgacgacg cacagcggta cggcctcacg
     3301 ctcacgcagc aggtgtaccg ctcggtggaa tcttttcgcc aggatgttcc agccgaatgg
     3361 gacatttact ttctggggca ggacaagaac cgcttcgata cgctgcgcgc cttggcgctg
     3421 cagtggcagc cgctgggttt gcgcacctgc attcgcatgg tgcccgaacc cggaagaagc
     3481 taccccgcca cgcccggcgt ggaagtgctg ccccgcagca ttggctacac cgacaacatc
     3541 gacacgatca accgctcacg ctgcctgctc gaaatcaccc aggccaacca gagcggcctg
     3601 accgtgcggt gcctggaagc cctgttcttc cacaagaagc tgcatggaca aggccgtgaa
     3661 cgaggcgggg gtagggtacc tgacattcca cggcttacgc cgcaccttca cccagacagg
     3721 ccggggtgtg gtgcccgctg gcatcgtggc ccaaatccaa gggcacaaat ccagcgccac
     3781 cgctgagggt tacgacattc gcacccttga cgagctccgc cccgagcttg agcgaatcga
     3841 ggcccacatc cttgcactgg ccggggtgca attcgacgcc caggctgaac cgggaaagct
     3901 gcgagtggtg agcgctgcat gaaaaacgca actacaaata atccttgaac ttcaaataaa
     3961 cgtagctaca ctaaaccatg aatgtgacgt tcgacccggc caaggatgcc gccaatgtgg
     4021 caaaacacgg cgtctcactg accgaggcgg caggctttga gtgggataca gccatagtgt
     4081 ggccggacac gcgcaacgac tatggagagg ctcgtatggt ggcgctgggg tacatcggtt
     4141 tgcgcatcat ggccgtggtg tttgttgacc gcccagccga caagccgaac gagcgccgcg
     4201 ttatcagttt gagaaaggcc aacagccgag aggtgaaacg ctatgccgaa acttaaagcc
     4261 ggaaccatcc tgccgacccc tgccgaggat gcagcaatta ccgccgctgc gctggcagac
     4321 actgacgccc agcccttcac ggatgctgaa tgggaacagg tcaagccgct ggcgcgccgt
     4381 ggccgcccgc tgggcagtgg cagcaaagca caggtcacat tgcgcctgga cgtggaggtg
     4441 ttggagaagt tcaaagcatc gggcgatggc tggcaaaccc gcatcaatga ggcgctgaaa
     4501 agctgggtgc ggacgcacgc ctgaaccgtt tttgcctcag ctaggcaagg tcgcaaactt
     4561 gccgaaaagc cggttcctcc cgctggtctg cttgggcttc tattaccggg agcaaccttc
     4621 gggaggatgc agtgggccaa cgcttgtatg actcggccac cgccgaagcc ttgatgaaat
     4681 tcgcttaccc cgagtatggg gtgcatgaac tcaagcccta cgagatcaag gagaacgggg
     4741 tgtacatcac ctcagctttt cgagacctgt gcccgcctgg aactgtcttt aactggacac
     4801 ctgccgacga aatgggatgg cctggcagcc ctaatcctga tacagcaccg ttcctgccca
     4861 tcccattcga cgctgcgcaa cttgccgccg caatgattga cggtgcaggc cgatccatcg
     4921 cgtattcgat ggagcatcgc cttggctatc cgctcgacga tgatgccctg aacaggtttt
     4981 cagaccgcat gcgatggatg catgatgcac ttgcagaagc ctaccaactg gccgctgctg
     5041 cgcaattggt cgttggtgaa ttcgaccacc aggccgaggc cagcgcctac gctttggctg
     5101 gtcaatacga agatgccgcc gacgcggcca acgagcgcga aggggtgttt gcaccagacg
     5161 tttccgaaga tgaacgccgc actcggcgcg atcgcgcagt cgcttctgtt ttgcctttac
     5221 aagtggcatt agacagtgcg atggacgcca gcgccaaaga gtggcggcag tggcgcacag
     5281 ccatggtgcg ccaactattg aacccagcaa cgcctgaagc cgcagcagca cagctcaagg
     5341 ccaacccgcc cgagcccttg atggcccaga gcgccagcac tgcgccgggc aatggcaagg
     5401 tgtggacgcc cgagagactg gcggatgcga aggcatatcg ggagaagcac ggcaccaaaa
     5461 agaccgctga acatttcgga gtttcagagc aattgattcg gcaaaaactg ccgagcgaaa
     5521 aggcaatccc gaagggatac agcgccttca cgcaccggcc caagtaagcg caaggggttg
     5581 cggtaggggt tgcgcttggt ggcttgaaag ccgcatgaaa taagggttcg cgtcccgcaa
     5641 tggggctgcg ccgttgaccg caacaggggt tgcgcccaaa tagtccagaa ccatgtttaa
     5701 cgaacccaga gggtttacac atggccgcta agactattca actcaccccg ctggcgctgg
     5761 aaacgcgcac tgcacttcct acacctgagg ctgcggggca cctcaatcgt gcgcaacaaa
     5821 cgctcagaat ttgggcgtgc cgcgaggatg gacccattcg ccccattcgc atcaatgggc
     5881 gcttggcctg gcctgtggcg gaactgcgcc gcatcctggg ggtggcgtaa tggaagcgct
     5941 gtaccaacac cccgacggca tgggcgcaat cgcctttgat gctgcaacgt cacgcctgtt
     6001 tctgctgaac gaagccgagg ccctgagcgc atgggcctat atcggcccga ccggcctgcg
     6061 cgtgctggcc tgctgcctgc tggccgctgc cgatgaagtg gaggcgaagc aatgagcgcc
     6121 ccaaaacaga aaaccccggc gccgtccaaa gcattcgggg tttccagcga acagctttcg
     6181 tttctgccgc cgccgccatt ttcgcccatc tggcccacga aaggaaccct tgccgaccgc
     6241 gcgctggggt acttcatgga cggcgcccgc atgaaccacc ccgagtttga ggcacgcacg
     6301 caaagctggc gactgggggc cgtggtgttc caactgcgca cgctgggctg gccggtggaa
     6361 accatcgaaa tcccgagccc gacggatgaa tgcccgcacc gcatcattgc ccgctaccac
     6421 ctggcgccgc accacgtcgc ccaggcgctg gcgcaactgg gggccgcgcg atgatggcgg
     6481 acaccgaacg cgcacgggcc gcgctgcaat ccatcccgcc cgactgcgac cgcgatacat
     6541 gggtgcgcgc tggcatggcc gcccatgccg ccggcctgga cttcgaggac ttcgacacct
     6601 ggagcgccca ggccggcaac tatgacgccc gcgccgcccg tgacacctgg cgcagtttca
     6661 aggccggcaa gggtgtaggc gctggcacgc tgcaccgcat ggccggcgag cacgggcacc
     6721 agcaagaaag gcccagcaag ccgcctaaac gggccgctac gccaccgccc gcgccgcgcc
     6781 caggcatggc ccctgctgac gtgtgggcgc gctgtgagcc cgctacgtgg gcgcacccgt
     6841 atatcgagcg caaggccgcc gctggcgtgc cgctcgatgc cctgcgcgtg gtgccccagg
     6901 gtgacgcgct gcgcatcggt gggcacccga tggccggcgc gctggccgtg cccgcctacg
     6961 ctgccgacgg caggctgcaa agcctgcaac tcattccgcc caagggcgag aagatgaacc
     7021 tggccggcgc acccatggcc ggcgcatcgt tcaccgtggg cacgccgggc gacgggccgc
     7081 tctacatcgt cgagggcatc gggcaagcat gggcctgctg gcaagcgacg ggccgcgccg
     7141 ccgtgtgctg tttcggctgg ggcaacgtgg gcacggtggc gaagcagctg caaggccgcg
     7201 ccctggtgct ggtgcccgac gtgggcaagg aggcccaggc cgaagccatc gcgcgcgagc
     7261 tgggttgcag cgtggccgcc atgccccagg gcgaggcgga caacttcgac tgcaacgacc
     7321 tggcgcaacg cgacggggcc gacgtgctgg ccgagctgct ggaatctgcc cagtcattca
     7381 agcccgaacc gctgctgacg ccggttgacg tggccggcgt gctgacgcac ccgagcccgc
     7441 cgccgcgctt tgtgtggaat ggctactgcc cgcgtggtgt tgtcacgctg ctgggggccc
     7501 atgggggcac gggcaaaagc accatcgccc tgatgctggc cgccgccgtg gcgacgggcc
     7561 gcgagctgtt cgggattgcc accgacgccg cgccggtggt gttcgccagt ttcgaggatg
     7621 gcgcgcacat cgtgcgccac cgcctggccc acatctgcca atgctggggc ctcgagcctg
     7681 ccgagctggc cggcctgcac atcgttgacg gcacggcaaa ccccgagctg ttcgcctgcg
     7741 agggacgcgg gccgggtgac gtgacacccg cctatgcgga gctgctggcg ctggtgaagc
     7801 aaaccggggc cgggctggtg attgtggaca acgccagcga cgcctacggg ggcgatgaaa
     7861 tccagcgccg gcaggtacgg gctttcatgc gcgcgctggc cgtgattgcc cgcgaaggcg
     7921 acgccgccgt gctgctgctg gcccacgtgg acaagggcac gtcacggcgc gagcgcagcg
     7981 acaccgaagg ctattcaggt tcgaccgcct ggcacaacag cgcccgaagc cgcatcttca
     8041 tgagccgcga cgatggcgtg ctgacgctgg cgcaccagaa aaaccaattc ggcaggatgc
     8101 gcgagccgct ggcgctgcaa tggcccgagg ggggattgcc gcaagctgca tcaagcaacc
     8161 cgttttcgga gcgcctgcag ggccgcgccg atgatgaacg cgccgccgcg ctgctgcgcc
     8221 tgattgtcga gtttgagggc cgcgaacagt attgcagtcc cgcattgttc gcccgcaaca
     8281 acgtgcacgc gctgctgaaa agcgaaccgg cgtttctggc cctgaagctg cgcgccgacg
     8341 atacccgccg catcgtgaac cagtgccagc gtgcgggctg gcttgaagtg ttggactacc
     8401 gcagtcccga caggaaagag cgccagaggt gggccgtcac ccaaacgggc cgggacttca
     8461 tcaacttgcc cgcgcccacc gcgcccaccg cgcccaccta tgaggatggc gcgcaaggcg
     8521 cagggggtgc gcccaccgcg cccacctacg tagggggtat gggggatagc gcgcgcacaa
     8581 atgagggcgc atcggctggt gcagaagccg gcctgactgc gcccacctgc accaccacgg
     8641 agggacacac ccatgattga ctgcctcatc caaggcaagc tgttcgccgc gccgcagcag
     8701 cgcaccagca aggccggcaa gcccttcgcc accgcaaagc tgattgcagc cgctggtgac
     8761 ggtgaacgcc tgttcgtgaa cgtcatagcc tttgacgccg cagcagtggc cgccctgctg
     8821 gcgctgggtg cgggtgacgc cctggccctg gccggcaccg caacaccgcg cgtctacatc
     8881 aaggacggcg agccgcgcgc aagcctggac gtgacggcgc agcaggtgct gaccgcccac
     8941 cacgtcgcgc gcaagcgcag cgccatgcaa gcaccagacc gcgctgcacc tgagcgtgac
     9001 cgacggtttg acgcttggga ggccgcccgt gctgactgac tacattgccc acgccctgcg
     9061 ctgcccacgc tgcaccgccg ccgcccgcct gcctggtgcc cgccgctgcc gccctggtgc
     9121 cgagctgtgg cgcaactacc tggccgcgct gccgccgccc gtgcagtacg acagcgacaa
     9181 gcgacgggcc gcacgccgca ccgcgcaggg gccgtgaccg ctggccgccc gtcaccccgt
     9241 caaacccgat tcattgaaag gaacaagcat ggaaacccag tacgacaagc tgatgcgccc
     9301 gcgtgccatc gcatccccgc ccgcccgagg cttcaatggg ccgctggccg caaagattgc
     9361 ccagcaccaa gccatcgccg ccgcgctggc gcaatgcccc ggcgcaagcc tggcaacgct
     9421 ggccgtatcc gagcacgagc gcaacgccat ccgcaatcga gcgggccgca tcgcccaggc
     9481 gcgccgtgag caggccgcac gccctggtgc gcctgcacgt cgataaccac caccgaaagg
     9541 acgaaccacc atgagcgaga aactcaaacc cctgtccgaa cacccggact atcacaacgc
     9601 tgccgagcgc ctggcgcact ttcaccgtga gctggccgcc gcccaggctg aggccgcccg
     9661 cattgacgta gagcgcctgg cagcgcctgg acaacgcccg gcagatgacc cgctcgcacg
     9721 ggctgatgca ctgctttctg gtgccgagcc gacgcccgcc ctgtccctgc gcgccggcaa
     9781 gaaccaagag ctgattgccg cgctgcgcaa ggccatcgcc gcccaggcca tcgtgctacg
     9841 ggacattgcc cgagcgcacg cggcagacgt tcgcgagcaa tcgacagccg agcacatcaa
     9901 gctggcgcag gccgtgctga acgccgccga tgcgctggtg caggcgaacg aggctgaggc
     9961 cggtttccgg caagaactcg ccgcgctggg ctatgacggc gcagtccccg gaatgagcta
    10021 cgcgccacca cctgagcgcg cagtcgattt gaccgagggc tatggcctgg cctggcgcac
    10081 cgaagtctcg gactactgcc gcacgggccg caccgccgct gaactggacg gagcgaagcg
    10141 cgacgccgat gccgccgtgc aggccgcagc gacagccgcc caggccgcac gcccgaagaa
    10201 ggcgccggca gttgacgcga gcaagggcga actggtgaac tgacgcggga gcagtccgtc
    10261 gggacttgcc cgacagccgg caggggcgtc aaccccgtgg ccggcacctt cacccggtgg
    10321 catggaaaag tctagagccc ttcgccgctg ggagccgccc tgttcctcac gcgcagatta
    10381 aatccaccgt ggagccgctt tcaagatggc ccgtaccgcc cgacatagcc cgaactgggg
    10441 aggcgcacgc ccaggcgctg gccgcaagcc ccgcccgccg ctgttggtgc aactgcccga
    10501 gcgtgccgac ccgctgcaat ggctactggc cgcgatgaac tgccccgccc tgccgctgcg
    10561 cctgcgggcc gatgttgccg ctttcctgct gccctactgc cgcgccagaa agtgaagccg
    10621 cccaggaacc gcgtacaagc ccgttgtggg cttttttctt gtctggtgtg acgtggatac
    10681 cgtgagcccc tgcgagcgca ccacggggcc gcaatcgagc gcgccgcacc cgtcaccacc
    10741 tgctggccgc tggcccttgc gtcaaggccg catatttccc gaagttgcct acagattgcc
    10801 tacagactac cgggaagaaa aaaccgcact acattttctg tagcgcggtt tccttatgtg
    10861 gcttggtggg tcgtgcagga ttcgaacctg cgaccaacgg attaaaagtc cgctgctcta
    10921 ccagctgagc taacgacccc cggtgccgat caaccgactt gcgtcagtca cttggcaagc
    10981 cgcagattat agcctgattt ttttgcccct cctgagacag ccgatcgagc acccagccgg
    11041 ccgcacacag gccgaaggtg gccgtgacgg cgacactgga gccgtagccg tggcagttca
    11101 gtgagctgtc gacctgctgg ggcgcctcgc aggcctcggg caggcgcacg ctctcgcggc
    11161 tgaacaccgc cgtcaccccg aggcgcttgc cgcccgtggg cgcgccgtgc tgacggcgca
    11221 ggcggtagcg cagctgggcc agcagcggat cgtgcgtgac ctcggcgagg tcggccacct
    11281 cgacccgttc accatgtcgc ttgccgccgg cggctccgac ggtgacaaag cacaggccgc
    11341 gctcgcgcgc ccaggccgcc atcgccagct tggccgacac ctggtcgcag gcgtcgatga
    11401 cggcatgcac cggcgccggc agcagctgcg gccagttggt cggggccacg aagtcctcca
    11461 ccgcatgcac cacgcaggcc ggatggatct gccctatgcg ctcgcgcatg gcctgcacct
    11521 tggcctggcc gaacgtcggt gtcagcgcat ggatctgacg gttgacgttg gactcggcca
    11581 catggtcgag gtcgatgagc gtgatctgcc cgacaccgct gcgcgccagg ccttcggcgg
    11641 cccaggagcc gacgccgccg atgccgacca ccgccacgtg ggcggcgcgg atgcgcgccg
    11701 cgccctcggc gccaaacagc cgcgccagac cgccgaagcg gcgttccaga tcggcctcga
    11761 cggccgccgc gctcacagcg atcgctccag gcgccagccc tggtatttca gcgccagcac
    11821 ggcgccgccg acaatgcccg ccagcgcggt gaagctggtc aggctgaggt tggagatgcc
    11881 gctcagcccc tggccgatgg tgcagcccag cgccgttacc ccgccgacac ccatcagcgt
    11941 gccgccgatc aggtggttgg ccagatcttc ggtggtgcga aaaccttccc agcgaaagcg
    12001 ccgcgacgcc agcgcatgca gcgccgcccc ggccaccagg ccgaacaccg ccaccacgcc
    12061 caggctcagg cgctggctgc ggtcgctgaa gaagatgaac cagtcgatgg tgtgcgccag
    12121 cgggccggtg aaggtcagcg cctccatgcg gccgctgcgc gtgcccaggt aagccggctc
    12181 cagcgtatcc gggtgttcgg gcaggtagcc caggtggcca ctcagccacc acagggcgac
    12241 gatgacaccg cccaggccca ggccggccag caggttgttg ccgttgatga agccgcgccc
    12301 ggtgagcgcc caggccatca gcaagccgcc caccagcagc gccgccccca gcaggcccac
    12361 cggcagcggc cagccgcccg cgctggcggc caggtgtccc agtgtggccg ggacgttgaa
    12421 cacccattcc acctggtcca ccgtgcggtc gcgcagcacc gccgtgatgc cgcgcaaggt
    12481 ggcgtaggac gccagcccca tgaccagaaa cacgatcagg cccttcaggt tgccgccgcc
    12541 caggcgcacc agcatcctgg cgccgcaacc cgaggccagc accatgccga cgccgaacag
    12601 cagcccaccc accaggcccg acagccacag ccagcgcggg ccggcgtaca ccgtgtcggc
    12661 cggctgcacc cagccaccgg cggccagcac gccgaaaccc agcgtcgcca ccccggcggc
    12721 cagcgcccac tggcgcagcc gggtggtctc gcccatgttc acgacgtcgc tgatcgcacc
    12781 catggtgcaa aagccggtgc ggtgcatgat agcgccaaac accgccgaca gcgcgaaggt
    12841 gccgagcagc acggcgcgca gcagcaggtc gaggtcgggc ggttgcggca tggggcgcga
    12901 ttctattgaa cctagaagcg gcagttggac gcgcccgccc gtgccgtgat cggcacgcga
    12961 cgacgccgcg gcctcgtgcc cgcaagtagg agcaacgccg catggcgcgg cgagcgcggt
    13021 gcgggcgtgc tcatccattc ggcgagcgcc tgccccgtgt tcgcccgccc cttgcgggtg
    13081 gcaaggcgtg ccgccccgcc gcgcctgatc gcagcaccca agcgctccga aatactatcg
    13141 ttttgatagc tttcagcgct tgccaatcaa gcgccagaag ccttttttac acttcattgg
    13201 gaggtgccgc ccaagcctgg gtgcccctgc gcgcgccgct agaatggacg gtttttgcgc
    13261 ccggccggct gcgctgcggc gcgttcctct ccgttcgcgg cccaggccgc ctctgccatg
    13321 tctgaatcgc tgtcgcaacc gggcctcgcg tccctgtcca agtccttcga acccgccgcc
    13381 atcgaggcgc gctggggccc cgcgtgggag caggcgggcc tggggcgggc gggctaccgt
    13441 ggcagcggcc agccggacgc cggcgccgcc gcgcgtggcg agaacttcgc cattcagttg
    13501 ccgccgccca acgtcaccgg cacgctgcac atggggcatg cgttcaacca gaccatcatg
    13561 gacagcctga cgcgctacca ccgcatgcgc ggcttcaaca ccgcctgggt accgggcacc
    13621 gatcacgccg gcatcgccac ccagatcgtg gtggagcgcc agttgcagga gcagggccag
    13681 agccgccacg acctggggcg caagaacttc gtcgcccgcg tgtgggactg gaagcagcag
    13741 tcgggcgaca ccatcacccg ccagatgcgc cgcatgggcg ccagcgtgga ctggagccgc
    13801 gaatacttca ccatggacga cagactgtcc aaggtggtga ccgaaacctt cgtgcaactg
    13861 tacgagcagg gcctgatcta ccgcggcaag cgcctggtca actgggaccc cgaactcaag
    13921 accgccgtca gcgaccttga ggtcgaaagc gaggaagaag acggctttct gtggcacatc
    13981 gcctatccgc tggccgacgg cagcggcgag cgcctggtgg tggccaccac gcggcccgag
    14041 accatgctgg gcgacgtggc ggtgatggtg caccccgagg acgagcgcta ccagcgcttc
    14101 atcggcaagg cggtgacgct gccgctggtg ggccgcgcca tcccggtcat cgccgacgac
    14161 tacgtggaca aggacttcgg caccggcgtg gtcaaggtca cgccggcgca cgacgccaac
    14221 gactacgcgg tgggccagcg ccacggcctg ccgatgatcg gcgtgctgaa cctggacgcg
    14281 acgatcaacg acaacgcgcc ggcgatcttc cgcggcctga accgctttgc cgcccgcaag
    14341 gccatcgtcg ccgacctcga ggcccagggc ctgctggtcg aaaccagaaa gcacaagctg
    14401 atggtgccgc gctgcgcccg caccggccag atcatcgagc cgatgctcac ggaccagtgg
    14461 ttcgtggcca tgaacaaggc ccccacgctc cctgctgcgc acggtgcgcg gcccggcgcc
    14521 ggggtcgcaa cgccgcctga tgctcgatcc atcgcgcaga aggccatcga cgccgtgcag
    14581 tccggccagg tgcgcttcgt gcccgagcag tgggtcaaca cctacaacca gtggatgaac
    14641 aacatccagg actggtgcat ctcgcgccag ctctggtggg gccatcagat cccggcctgg
    14701 tacgacgaag agggcaacgt ctacgtcgcc cgcaacgagg ccgaggcgca ggcccaggca
    14761 cccggcaaga cgctgcgccg cgatgaagac gtgctggaca cctggtactc gtcggccctg
    14821 gtgcccttct ccaccctggg ctggcccgaa ccgaccgagg acctcaacct gtacctgccc
    14881 agcagcgtgc tggtcaccgg ccacgacatc atcttcttct gggtcgcccg gatgatcatg
    14941 atgaccacgc acttcaccgg ccaggtaccg ttcaaggacg tctacatcca cggcctggtg
    15001 ctggacgcgc acggcaagaa gatgagcaag agcgaaggca acgtgctcga cccggtcgat
    15061 ctgatcgacg gcatcgcgct ggagcccctg ctggccaagc gcaccaccgg cctgcgcaag
    15121 cccgagaccg cgcccaaggt gcgcgagaac accaagaagg aattccccga cggcatcccc
    15181 ggctacggcg ccgacgcgct gcgcttcacc tttgccgcca tggccacgct ggggcgcacc
    15241 gtcaacttcg acagcaagcg ctgcgagggc taccgcaact tctgcaacaa gctctggaac
    15301 gccagccgct tcgtgctgat gaactgcgaa ggtgccgact gcggcgtcga gggcgacacc
    15361 gcgatcgagc gctcggccgc cgaccgctgg atcgtcagcc tgctgcagca gaccgaagcc
    15421 gccgtggccc agggctttgc cgactaccgg ctcgaccagg tggcgcagac gatctatgac
    15481 ttcgtctgga acgagtactg cgactggtac ctggaaatcg ccaaggtgca gatccagacc
    15541 ggcaacgcgg cccagcagcg cgccacgcgc cgcacgctga tccgtgtgct ggaagccatc
    15601 ctgcggctgg cccaccccgt catccccttc gtcaccgagg cgctgtggca ggtggtggcg
    15661 ccgatcgccg gcaaggcggg cgacagcatc gccacccagc cctacccgca ggcgcagcca
    15721 gtccacatcg acgaggcggc gcagcgccag atcgggcatc tgaaggcgct ggtcgacgcc
    15781 tgccgcaacc tgcgcggcga aatgggcgtc tcacccgccc agcgcctgcc gctgtacgcg
    15841 ctgggcgagg ccgacttcat ccgggaccag gcgccggtgc tgcaggcgct ggccaagctg
    15901 agcgaggtga aggtgttcga gcacgaagcc gactgggccg ccgccgcgca ggcggcgccg
    15961 gtggcggtgg tgggtcaggc gcggctggcg ctgttcatgg cggttgacgt ggctgccgag
    16021 aaggcccgtc tgggcaagga agcgggccgg ctggaaggcg aaatcgccaa ggcacagggc
    16081 aagctgaaca acccggcctt cgccgccaag gcgccgccgg ccgtgatcga gcaggaaaac
    16141 cggcgcgtgg ccgaattcgg tgcggcgctg gccaagatcc gcgatcagtt gcagcgcctg
    16201 ggctgaaagc ggcccgtcgc gccggccggt cggcaggcaa gaagggattc cgggcgcccg
    16261 agggggagcg ccgccgcgtg gcggtttcag gcgccaggca ccgccgtgac ccgggccacg
    16321 gccagcatgg gcaacaacca gcgtgccttg ggggcggtcc aggcgggcat gggctgcccg
    16381 cccacacctt ctcagtccag cggaaccggc tcgctgtcca gtgtgccgcg ctggctgcgg
    16441 gtttcgttca gcatcagcgc ctcatggatg cggtcgacca ggtaccagct gccgcgcagg
    16501 tgcgattcct gcgtgtaggg cgccagccgg ggcgtgatgc gcacgttggg cgcgtgacgc
    16561 agcggcgaat cgggggccgt caggcgctcg tcgtcgctgt ccatcatgag cgcgccgatg
    16621 cgccccgagc gcagcgcgcc ggccagggcg tcgaggtcga acagcgacgg gcggctgatg
    16681 ctggtccaca gctgccccgg cttgcaggct gccagcacac gatcgttgac cagaccccgg
    16741 tagcgtgagg catagatgac ctgcatcgac accgcgtcgg ccacctccag catgtcggcc
    16801 agcgccatcg gctgcacgcc cagccggcgc cacagttcgg cgttgcggtg caccgccggg
    16861 tcgtagccca ccacgcgcgc acccaacggc accagcatgg tggccagcac gtgcgccggc
    16921 ggcgccaggc cgaacaggcc gatcacgctg tcgttgatct cgcgccccgg tgcgccacga
    16981 cgcagcgaca gcgagggtcc aacctgcccg ccggagcgaa acagctgcat caaggtgcac
    17041 agctggtgct cggcgctggc gcgcacggtg gcgctggagg cctggatcac acgcacatgg
    17101 cgcttctggc aggcttcgaa atccaggttc tcggtaccgt cgtggatgcg gcccacggcc
    17161 gccaggcgcg gcgcgtagtc cagcagctgg gcgttgattt tgaggcgcgg cggcaccacc
    17221 agcgcgtcga ccttgtacag cctaccgcgc agcaaggcct cgtcctccag cagctctggc
    17281 cgataatcca cgtggtgccg cgcttccagc cactggcgtg cgtcggccac cagttcctcc
    17341 agcaggagaa tatccaagcg ttccctcttc gagttgtcgt tttgacctgc cttgccggcg
    17401 tgtgctggcc gtgtcagagg ggcagacagt cagcttgtca ctttaccacc ccgccacccg
    17461 atcatgaccg catcgcgcca ggttctcacc gccgtgttcc ccgtcgccgg cctgggcacg
    17521 cgcttcctgc cggccaccaa ggccagcccg aaggaaatgt tgcccatcgt cgacaaaccg
    17581 ctgatccagt acgccgtcga agaagcctac gccgccggcg tgcgccacat ggtgttcgtc
    17641 accggccgca gcaagcgcgc catcgaggac cacttcgaca ccgcctacga actggaaagc
    17701 gagctggagg ccggcggcaa ggccgatctg ctggccatgg tgcgcgccgt gcagcccgac
    17761 gacatggact gcacctatgt gcgccagccg cgctcgctgg gcctgggcca tgccgtgctg
    17821 tgcgcggcgc acatcgtggg cgagcagccg ttcgcggtgc tgctggccga cgacctgatg
    17881 gtcggcccgg ccgacggcca gccggtgctg gcgcagatgg ccgacgcctt cgagtcgctc
    17941 ggccgttcgg tgctggcggt gcagcaggtg ccggccgatc aggtgcgccg ctacggcatc
    18001 gtggccggca cgccggccgg cgagcggctg atcagcatcg aacgcatcgt cgaaaagccg
    18061 gcccccgagc aggcgccttc gcgcatgggg gtggcgggcc gctacatcct gacgccgcgc
    18121 atcttcgacg agatccgcca gcagccgccc ggggccggcg gcgagatcca gctcaccgac
    18181 gccatcgccc gcctgatgca caaagagcgc gtctacgctt accagtacag cggcaaacgc
    18241 tacgattgtg gtagcaaaga aggctttctg gaagccaccg tggagctggc cctgcagcac
    18301 ccgcagatgg gcgcgccgtt tcgcgactac ctgaagacgc tcagcctctg acgcgggacg
    18361 cgacggtggc ggcccgcttg cgctcttggc gtgcgccgca gggcctgcgg ggcggcgtgg
    18421 cttcgtcggc cagcgcctgg cggacgcgtt gcgcggccgt cgctgggccg acggatcagc
    18481 gccgccgcag tacgtgggcg gtcacctcgc cctcggtgct ctgctcgacc agttcgttgc
    18541 cggtctggcg ggcaaaggcc tgaaagtcgc gcagcgcgcc gccgtcggtc gacagcacgc
    18601 gcagcagttg cccgctttgc agctcggcca gcgccttttt ggccttgagg atgggcagcg
    18661 ggcaattcag cccgcgcgcg tcgacttcct tggcgatatc catcggacga ttgtcggtca
    18721 atccagaccg cggcgtcgct cggcgttttc ttcgggcgtg aaaaataccg gctcatgccc
    18781 gcgcgcgcgc agccagtcgg ccagggcgta gccggtgccg gccggccagc acttgagctg
    18841 gggcgggtcg tacaggcgcc agtcggccag ttcgggcgac agccgcacct cgccctcgca
    18901 gacggcatga aaggcgatga tgacctggtt catgcgctgg aagtcgtaca cgcccaccag
    18961 cgccagcgag cgcgtgtcga gctgggtttc ttccttgatt tcgcgcacga tgccgtcctg
    19021 cggcgtctcg cccgcctcca tgaagccggt gatgagcgcg aacatcttgc tcggccaggc
    19081 ggcgttgcgc gccagcagca cctggccgcg gtattcgatg atggccgcca gcaccggcac
    19141 cgggttgttc cagtgcgtgt agtcgcaggc cgggcagcgc aggcgctccc tggggccgct
    19201 gtcttcttcc tgcacgatca tggccagcgg cgtggcgcag caggggcaat agcggaacgg
    19261 gctcatgtta ctatattatt catagctgct ggcgcttgtc agtaaagcgc cagcggccgg
    19321 ttggattgtg aaagcctcag gccgggaaca cgccggtgga caggtagcgg tcgccccggt
    19381 cgcacaccac gaagaccacg gtcgccccct gctcgcgcgc cgccacctgc tgcgccaccc
    19441 aggccgcgcc gccggaggag atgccgccga atatgccctc ctcgcgcgcc agccggcgcg
    19501 ccatgtcctc ggcgtcggcc tggctgacca gggtcagctc gtccaccgcg gcggggtcgt
    19561 agatgcgtgg caggtattcc tcgggccact tgcggatgcc cgggatgcgc gagccctcgg
    19621 ccggctgcgc gccgatgatg cgcacggccg ggttctgttc cttcagaaag cgcgacacgc
    19681 cggtgatggt gccggtggtg cccatggccg agacgaagtg cgtgatgcgc ccgccggtct
    19741 gctgccacag ctcggggccg gtggtttcgt agtggatgcg cgggttgtcg gggttggcga
    19801 actgatccag cacgcggccc ttgccctgcg ccaccatctt gtcggccagg tcgcgcgcgt
    19861 actccatgcc gcccgagcgc ggggtgagga tcagctcggc gccgaaggcc ttcatggtct
    19921 gggcgcgctc gaccgacagg tcctccggca tgatcagcac catgcggtag ccgcggatcg
    19981 ccgctgccaa ggccagcgcg atgccggtgt tgccgctggt ggcttcgatc agggtgtcgc
    20041 cggggcggat ctcgccacgt tcttcggcgc ggcggatcat cgacagcgcc ggtcggtcct
    20101 tgaccgagcc ggccgggttg ttgccttcca gcttggccag caccacgctg ccgcgcgcgg
    20161 cgtgctcctg cgcgcccagg cgctgcagcc gcaccagcgg cgtgcgccca atcgcatcct
    20221 cgatcgtcgg gtagtccatg ccatgcactg tgccataatt tttggctgtg ctatcgcgct
    20281 gcccgggtgg cgaaatcggt agacgcagca gactcaaaat ctgccggtgg caacaccgtg
    20341 ccggttcgat tccggccccg ggcaccacat tgccgcaagc cgcacgcctg catcgggcgt
    20401 gcggcttgcc tgctttcaca ggctttcggg cggcatccag cgctgcaggt gcagcacgtt
    20461 gtcgccgcgg tgcgcgcggc tgcggcgcag ctcttcgcgt gccacggtgc gcagatgcac
    20521 ttcatcgggg ccgtccagaa agcgcagcgc ccggccccag ctccacagtg cggccagcgg
    20581 cgtgtcgggc gtcaggccga cggcaccgaa cacctgcatg gcgcgatcga gcacgcgcgt
    20641 ctgcaactgc gctgccacca ccttgatggc cgagacgtcg gtgcgggcgg cggcgttgcc
    20701 ctcggtgtcc atgcgccagg cggcgcgcag caccagcagg cgcgcctggt cgatttcaag
    20761 ccggctgcag gcgatccagt cctgcacgtt ggcgaagtcc gacaaatgcc tgccgaaggc
    20821 catgcgctcc agggcgcgct cggccatcag ttccagcgcc agctcgcact ggccgatggt
    20881 gcgcatgcag tggtgcacgc gccccggccc cagccgcgcc tgggccatgg caaagccctc
    20941 gccggcggtg cccagcaagt gatcggccgg cacgcgcacg tcacgaaaca ccagctcgca
    21001 atgcccttcg atggcctggt gctgcatcac cgggatgttg cgcacgatct ccagacccgg
    21061 cgcgtccatc ggcaccagca gcaggctgtg tcggcggtgc gcgggcacat cgggggcggc
    21121 atcggacacg cccatcacga tggccaggcg gcattggggg tgcgcggcgc cggtgatgaa
    21181 ccacttgcgg ccgttcagca cccagtggtc gccatcgcgc gcaatgcgtg tgcccaggtt
    21241 ggtggggtcg gatgaggcga cgtccggctc gctcatggca aagcaggagc gcatcgtgcc
    21301 ttccatcagc ggccgcagcc agtgcgcgcg ctgcgccggg gtggcgaaca gctgcagcag
    21361 ctcgatgttg ccggtgtctg gcgcgctgca gttgaacacc tcgctggccc agggcacgcg
    21421 acccatgatt tcggccagcg gcgcgtattc gaggttgctc aggcgggtgc cgggttcgtc
    21481 gtcgcgcaga ccgggcagga acaggttcca cagaccggcg gcgcgggcgc gcgccttcag
    21541 cggctcgatc acgtcgagcg ggtaggcacc ggcctcggac cagcgcagcc agtcgcggtg
    21601 cgaaggcagg atcaggtcgt ccatgaaacg ctgcacctgc tgacgcaacg cctgcacccg
    21661 ggtcgaatag gcaaagtcca tggacttgac tgtacgcaag cagcaccacg gcgccagtcc
    21721 tgtgcacgac acgggcctgg ccaagtctta tgatcaaatc aggctctcgc gcttgtccag
    21781 aaagcgccag cagctatgtt tttggtagca atccggtgtg cacgtcgggg tggcgcaggc
    21841 gcaggcgcag ctcgcgcttg aggcgcgtgt tgtgcatgcg gcgcgattcg ctcatgaacg
    21901 acagcagcgt tgccggcagc tgcgcgtctg cggcctggcg gtcgatgcgc ggcggccgcg
    21961 gcaggccgta caggtcggcc gccagatcga cgtaatcgcc catcttcagc tggctgtcgt
    22021 cgcaggcatg caccacgcgc tgcggccggc cgcgccacag cgccagcaca aaggcgcgcg
    22081 ccagatcgtc ggcgtggatg tggttggtga acacgtcgtc ggccggcgcc agcaccggcg
    22141 tgccccgctg caggcgctcg cgcggcgtgc cgccggggcg gtcgggcgcg tagatgccgg
    22201 gaatgcgcag gatgctggcg cgcacgccgg cggcgcggcc aaaggcacgc acccaggttt
    22261 cggccgccac gcggcgccgc gcacgcgccg tctgcggccg cactgggcgc gtctcgtcca
    22321 cccaggcgcc gccgcagtcg ccgtacacgc cgctggtgga gccatacacc agcgccagcg
    22381 gcgcgcgccg ccgggccagc gcgcgcagca gcgcggcggt gcgcgggtcg ccctcaccca
    22441 ccgcgggcgg cggcgccaga tggagcacat gggtggcgat gccggccagc cggtccaggc
    22501 tggcggcctg gtccagatcg ccggccagcg gcacgatggc ctgctggcgc agcgccgccg
    22561 ccgcctcggc cgagcgcgcc agcgccagca cgcgcacgcg tggcggcaac gcccgtgccg
    22621 cccgcgagcc gacgtcgccg caaccgacga tcagcacgcg cgggcgccga aagcgcgccg
    22681 gcagcgcgcc gtaagggttc tggtttgagg gcaaaatctt cgctttcttc cgccatgcaa
    22741 gaccgccaca ggataaccaa ggcatgaccc acaccatcac cgtcgaaccg agcggccgca
    22801 gctttcaggc cgaagcgggc gaacccatgc tcaccgccgg catccgccag ggcatcggcc
    22861 tgccctatgg ctgcaaggac ggcgcctgcg gctcgtgcaa ggtgaagaag ctctccggcc
    22921 aggtgacgct cggcccgcac caggacaagg ccctgtcggc ccaggaagaa gccgacggct
    22981 acgtgctgac ctgctgcggc gtggccacca gcgacgtggt gctggagtcg cgccaggtca
    23041 cgcacgccgg cgcctttccg atcaagaaga tgccggcgcg cgtggcgctg ctggagcgcc
    23101 tgtcgcacga cgtgatgcgg gtgcggctgc aactgccggc caacgacatc atccagtacc
    23161 acgcgggcca gtacgtcgaa ttcatcctgc gcgacggcag ccgccgcagc tacagcatgg
    23221 ccaatgcgcc gcacacgctg atcgaaagcg gctcgcccat ggtcgacctg cacatccgcc
    23281 acatgccggg cggcaagttc accgaccatg tgttcggcgc catgaaggaa aaggaaatcc
    23341 tgcgcatcga agggccctac ggcagctttc acctgcgcga cgaatcgccc aagccgatcg
    23401 tgctgctggc ctcgggcacg ggttttgcgc ccatcaaggc catcatcgag cacatgcagt
    23461 tcatgggcat cacgcgcgag gccgtgctgt actggggtgg ccggcgcccg caggacctgt
    23521 acctggacgc ctgggtgcgc gccaagacgc aggagatgcc gcacctgcgc tacgtgccgg
    23581 tggtgtcgga cgcgctgccc gaagacggct ggggcgggcg caccggcttc gtgcaccgcg
    23641 cggtgctgca ggatctggcc gacctgtcgg gccatcaggt gtacgcctgc ggcgcgccga
    23701 tcgtggtcga ttcggcgcgc cgggactatg tcgagcaggc cggcctgccg gccgaagagt
    23761 tctacgccga cgccttcacc agcgaagccg acaagcacgc cggctgacgg cagtctgtgt
    23821 cgccggggct ttccctgcgg tggcccggcg ttcgtccaca ccttcgggca cgacaggtca
    23881 gcgcacgcgc tggcgatcgt gcttctggcg atggatacga cggctgttgc gatcctgcat
    23941 ggcgtgcaga cgggcgcgct cggtggcggt cacccgcccg tccgccttgg cgtcagcttc
    24001 ggccgccgcc acccggttct gctggcgctg catgcgcgcc gcctcgcgcg gcgtcagctg
    24061 acccgaggcc acgccctgct cgatgcgacg ctcctgattg atctgacgct gatccacgcc
    24121 cggggtggcg accccttgcg catgggccac gcccacaccc atgcacgccg tggtcacggc
    24181 cacgctcagc catcgattga tcatgatgtt tctccttcaa gtgtctgatg gaacgggtcg
    24241 gcgacccgcc cacactgcac aacgcagggc gcggcgcccc gccgacgagg cgcaggcaaa
    24301 gctcgcgtaa agttcgtgcg tccgttgttc acttcttttc acacgctcat gaccatgaat
    24361 cgccgctcgc tccttcctgt tctgtcggtg gctctgcttg ccagcgccat cggcgccgcg
    24421 cccgcggcct gggcgcaagg caagccgatc cggctgatcg tgccctacgc ccccggcggc
    24481 ccgctcgacg tcacggcccg cgcactggcc gagcgggtgc agcccgcgct cggcaacgtg
    24541 gtggtcgaaa acaaacccgg cgccggcggc aacatcggct ccgacctggt ggccaaggcc
    24601 gcgcccgacg gcagcaccat cggcatcgcg gcggtggcca cgcacgccat caacccctgg
    24661 ctgttcagca agatgcccta cgacgccggc aaggacttcg cccccatcac ccagatggtg
    24721 cgtgtgccca acgtgctggt catcaacgcc gacaccgcgc agcgcctggg catccgcacc
    24781 ctggccgacc tgatcgccta cgcccgcaag aaccccggca agctgaacta cggctcgggc
    24841 ggcaacggct cggcgggcca cctggccggc gagatgttca agcagggcgc gggcattttt
    24901 gccgtgcaca tcccgtacaa cggcgccaac ccggcgcagc tggcactgct ggccggccag
    24961 gtcgactaca acatcgacaa cctggcggcg gcggcggcca acatcaaggc cggcaagatc
    25021 aaggcgctgg ccgtcaccag cgcccgcccc tcgcccctgc tgccgggcgt gcccgccatg
    25081 gccgagacca tcaagggctt cgaggtcgat acctggtggg gtctggtggc gcccgcgggc
    25141 acgccgcgcg aagtgatcga ccggctcaac gccgccttca ccgacgcact caaggcgccc
    25201 gaaacgcagc agcgctttgc ccagctgatg gccgagccgg tgccgaccac gcccgagcaa
    25261 ttcggccagt tgatgacgcg cgagcgcgcc cgctaccagc cggtggtcaa gcgctcgggt
    25321 gccaaggtgg actgacccta gaaacggcag cagcccgccc cgaaccacaa aaaagccacg
    25381 ccgccagggc gtggctttgg cgttggggca cggcagcggc gttattcgcc caggtaggcc
    25441 gcgcgcactt tcgggtcggc cagcaggtcc ttgccgctgc cgctcatgct gaccaggccc
    25501 gactccatca cgtagccgcg gttggccagc gccagcgcgc ggctggcgtt ctgctccacc
    25561 agcagcacgg tgacgccctg ggcgtagacg tcctgcacca cctcgaaaat cttgtccacc
    25621 atgatgggcg acaagcccat ggacggctcg tccagcagca gcaccttggg ccggcacatc
    25681 agcgcccggc ccatggccag catctgctgt tcgccgcccg acatggtgcc ggccagctgg
    25741 tccttgcgct cacgcaggcg cgggaagatg ctgaacacgc gctcgatgtc ctgcgccaca
    25801 ccggccttgt cgctccggat gtaggcaccc atcagcaggt tttcggtgat gctcatgcgc
    25861 gtgaacacgc cgcggccttc gggcaccatg gccaggccct ggcgcaccag atcccacggt
    25921 ccctggccct tgatggactt gcccaggtac tcgatgtcgc caccggccag cggcagcgag
    25981 ccggtgatgg ccttcatggt ggtggtcttg ccggcgccgt tggagccgat cagcgacacc
    26041 aactcgcctt cgcgcacctc gaagtccacg cccttgacgg cctggatgcc gccataggcc
    26101 accttcaggc cgctgacttt caacagtgtc tgtgccatgt cagtgacctc ccgtgcccag
    26161 ataggcctcg atgactttct cgttcttctg cacctcggcc ggcgtgccct cggcgatctg
    26221 cttgccgtag tcgagcaccg tgacgcggtc gcacaggccc atcaccagct tcacgtcgtg
    26281 ctcgatcagc agaatggtgc ggccgtcctt gcggatgcgg tcgatcagcg cgcgcaactg
    26341 caccttctcg gtggcgttca tgcccgcggc cggctcgtcc agcgccacca gttgcgggtc
    26401 ggtggccagc gcgcgggcga
//
LOCUS       DAOOOG010000015        25372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_1828_length_25372_cov_23.412608, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000015 DAOOOG010000000
VERSION     DAOOOG010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 25372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25372
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1828_length_25372_cov_23.412608"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="PK242_02495"
     CDS             <1..1527
                     /locus_tag="PK242_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ92919.1"
                     /translation="GQDVLLLDNDDTVSIGSRGVCYAKRALEVLDRMGCGDKFVDKGV
                     SWDVGRTFLREGEVFNFNLRPEADHHRPGMINLQQYYLEEYLVERARQLPRLQMRFKN
                     NVISVVPGADQVTVRVETPDGPYSLTTDWLVVADGARSPIRTMMGLDIEGKIFMDRFL
                     IADVVMKADFPAERWFWFDPPFHPNQSVLLHKQADNVWRIDFQLGWQADPVEERKPEN
                     VIPRIKAMLGDEREFELEWVSIYTFQCRRMNSFNHGRVLFVGDAAHQVSPFGARGANS
                     GIQDTDNLVWKLKLVMDGKAPPTLLDSYTEERGFAADENIMNSTRSTDFITPKSNTSR
                     TFRNAVLELAAQHPFARALVNSGRLSVPSWLASSSLNTPDTDAFQGQMIPGAPMADAP
                     VRTAGGDGWLLHQVGNRFQALHYVDDAAALDTDTTRALSQLASHGIDVEPIVVARRGQ
                     APAGIRTLIDGKGCMAQRYDLQPGTTYLLRPDQHVAARWRTLNAASVKAALARATGNT
                     "
     gene            1554..1778
                     /locus_tag="PK242_02500"
     CDS             1554..1778
                     /locus_tag="PK242_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HOZ92920.1"
                     /translation="MPLNTQPNFSEAGRRYFQAYSPGDDFYEALIDTHRDLSDEQSAM
                     VNARLILLLANHIGDIGILRQAMQIAREGV"
     gene            1820..2374
                     /locus_tag="PK242_02505"
     CDS             1820..2374
                     /locus_tag="PK242_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ92921.1"
                     /translation="MSMLKGVHHVAYRCKNAKETVEWYKKYLDAEFVLAIAENEVPST
                     KEPDPYMHIFIDLGGGNILAFFELPTRAPMIAPSDANTPSWTQHLAIKVDSLDTMMKA
                     KARLEADGIEVIGPTDHTIFKSIYFRDPSGHRLELAVNTATPEMDKALDDVKWDMLEE
                     WDRTKKAPTHARWMHDGSGRVGPH"
     gene            complement(2467..4398)
                     /locus_tag="PK242_02510"
     CDS             complement(2467..4398)
                     /locus_tag="PK242_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOZ92922.1"
                     /translation="MIGIVQRVLRAGFMGAEAVFNRAFGDRMNPFYHLGKISFFLFWI
                     IGATGLILYAFFDTSVEGAYQSVETITHSLWGLGGVLRSLHRYASDAMVLTMLLHMLR
                     YFAFDRLRGFRAFSWVTGVGLLWLTYVAGVNGFMLPWDRMAQFVTQASFEWMDWLPGF
                     KGRLIRNFILPEHVSNRLFSLLVFIHIGVSLLLLLVMWIHVQRVPKAAMHPPRPITVA
                     LSVTLLVLSLAVPVFSQGPADLHTMPLRLSFDWFLLTVFPLIQSGAMGAVWVLLVGST
                     AFGVMLPWLTLRRSAHAPRQLTIHPGPARVMARAGETLLDAGLRAELALPYDCRAGGC
                     GVCVCTVLNGKVDLGAYQDAVLTPAMRARGQALMCCAVALEDVEIEVEGVATLARDEA
                     SELRHLQARVQRLERISPTVMRVVLALPAGQTLPFTAGQYINIILEDGAKRAFSFANA
                     PADPAQPFPLPTDNLIELHVRLIEGGRFTTHVFEAMKEGDVVDFEGPIGRFTLRDSER
                     PILMIAGATGFAPIKSILEDAFRRGIRRPIELYWGVRSQEDLYLQDLLAQWQERHANF
                     RYVPVLSDLAPDDPWPGRRGFVHQALLADHPDLTGYEVYACGSTRMVEAAVPDFIAHG
                     LGEQFCFSDAFLPTAGQGS"
     gene            complement(4395..5870)
                     /locus_tag="PK242_02515"
     CDS             complement(4395..5870)
                     /locus_tag="PK242_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOZ92923.1"
                     /translation="MNPDTAWTPKNLRLPSPTVRAERVTASTPARFELPAVTAAGRSL
                     ARGWLWLALGALLGSGLFSVLLVLARTPGINAWLPAGDFFRVALVVHVDLSVLVWFVA
                     IAGLLWSLNQRPPGSPRSRLLLRAPLWLCGLGALGMGLAAFVDPGEAVMANYIPMLDS
                     AGFRAALWVFAAGALWLVLLTMARPVPIGPAVDGTGALRFGLHAGAVAAGVALLAFAW
                     SLVVVPTTLPAKAYYEILFWGGGHALQFTWTLLMLVAWLVLVQSCGGRVPLSPRIVVV
                     LFLIALVSVFVTPLAYLMHDVSSVEHRDMHTWGMRFGGGVAIAPLVLAVLLALLPMRG
                     LAPVQRPLRAAVLASMLLFIAGGVIGLTIQGSNVKIPAHYHGCIVGVTLALMGLVYRL
                     LPELGYAAPRGRLAVAQPWLYGVGQLLHIVGLMWSGGYGVQRKVAGAEQVLRSTGEIA
                     GMGLMGLGGLLAIVGGLLFALVVVRAMRAEPTAGLDRRAAP"
     gene            complement(5867..6418)
                     /locus_tag="PK242_02520"
     CDS             complement(5867..6418)
                     /locus_tag="PK242_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92924.1"
                     /translation="MTTPSRPEPREGRSTAAPGAAQWAGQVLLYGLFALAIGVFSQWP
                     TYRPIDSDQAVIKISVTRLGQPVGECRRLSPDELAKLPPNMRDPLQCPRERSPLTMEV
                     DVGGQRVLQRVAPPSGLSKDGSASIYERLVVAAGPQQIDVHFKDDVRPGAVTYHRQAS
                     VNLVPGQVLVVDFDAEKGGITLQ"
     gene            complement(6396..7967)
                     /locus_tag="PK242_02525"
     CDS             complement(6396..7967)
                     /locus_tag="PK242_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOZ92925.1"
                     /translation="MSEHAPASIRSWGLSGWRALERLFDSAFGSGLNPLRHLGALGFL
                     ALWLLVASGIVLFILFDTSVTGAYASVEGLARLPLGLGHLLRGLHRYAADVLMLVMLL
                     HIVREWLHGHERGVRRFHWLTGVPLVAFCFVSAIGGFWLIWDQLSQYSALATAEWLDR
                     LPFLTSPLARNFLTAQAVSDRLFSLFIFIHLGVPVLLLFGLWFHIQRLAHATVLPPRA
                     LMLGLLGVLALLALARPVVSHAPAALGRVPAALQLDWLLLWLHPLTDVTSAAFTWALV
                     IGAMLLLLALPWLPQPARAAVAVVNPDHCSGCRRCLVDCPYAAITMVPHPDRRAGREL
                     AQVDADLCVGCGICAGACPSSTPFRRIEQLVTGIDMPQLPVDALRQRLRQDLSRATAA
                     QPLVVFACDHGAAAGGAAAGDVSTYSLLCTGQLPPSFVEYALRDGAAGVVVATCRAGG
                     CEFRLGQRWTLERMQRQREPHLRQHIPAERLELVAAGPGDAVELRAAVERLRVRVHGL
                     SDLPRIHHDDYTLQT"
     gene            complement(7925..8830)
                     /locus_tag="PK242_02530"
     CDS             complement(7925..8830)
                     /locus_tag="PK242_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOZ92926.1"
                     /translation="MATRLLRGCVAVGAIWLALAGGPALADGPGAAARAPGEVPSLDV
                     GEAVRLAEAAVGRTVPDFEMRDRRGKPVRLSDFRGKPLLVSFIYTGCFEICPTQTRTL
                     YESVKGLDVMLGQDQFNVVSIGFNQPFDAPEAMRAFAAQHRIQHRNWEFLSPRADQVD
                     ALTRAFGFSWVATPAGFDHVLGVTVVDADGRIHSQVLGDIVRADRLGVPLRRLLLYDQ
                     PLPALGQFENIVERVRILCTVYDEETGEYRYDYKLILVVLSGVLFFLSTLIYLGLEWR
                     NQRRQRRQLRAPCPSTPQPASGHGV"
     gene            complement(8850..9767)
                     /gene="cyoE"
                     /locus_tag="PK242_02535"
     CDS             complement(8850..9767)
                     /gene="cyoE"
                     /locus_tag="PK242_02535"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOZ92927.1"
                     /translation="MNRVDPSAAAMVMATRPATAGWRLVLSVFKLRIGTLIMLTAIAG
                     LAIVPDGGATPWQSLVLALSVLVASASAGAFNQYVEADSDRLMARTRTRAFASGALQR
                     GPGWLVLMAAMLAGAVGAAAWWVNLPAALFVLLGALTYAVVYTLGLKRRTPWNIVIGG
                     AAGGFAALAGAAAANPQVGPLGLLLALVLLLWTPPHFWSLAIANEAQYAAAGVPMLPV
                     VVGKARAARTVHRCTVVLVAVSLLPLAFGAGWAYGLGAVVGGLNFLRKTAALARAPHE
                     RARAMGAFFASMMQLSAIIAGVLIDAALR"
     gene            complement(9826..11526)
                     /locus_tag="PK242_02540"
     CDS             complement(9826..11526)
                     /locus_tag="PK242_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOZ92928.1"
                     /translation="MTAVATTYRTCPRSGLQFERQAEMLIKAHAFVGVVWLLIGGLLA
                     IGVVLTRWPAVRWLEADTFYMVLTAHGLDMLIFWIIFFEIAILHFCSSTLLRCRIATP
                     KIAWLGFALMLIGSILNNVAVFRGGSSVMMTSYVPMMAEPSFYLGIILFAVGALIGCF
                     VFFGTLVVARREKTYEGSIPLVTFGALTAAIIAVFTLLSGAMIIVPAWLMSLGLMNVD
                     PLIYRTVWWALGHSSQQINVAAHVSVWYLVAAIALGAKPMSERVSRTAFLLYILFLQL
                     ASAHHLLADPGVGTAWKVVNTSYFMYLAVLASMLHGLTVPGAMEVAQRQKGYTKGLFE
                     WLRKAPWGNPTFSGVFISIIGFGFLGGITGVMMGTEQLNLIIHNTIYVPGHFHATVVV
                     GTTLTFMALTYYLIPVLFKREMIAPKLAQWQPYLFGFSMYFFCLVMMGAGTLGVSRRH
                     WDMAFQGAALAYEWPGAAYLMMALVGIGGIAAIAGGAIYIYITVGSLLWGRKLDAGAS
                     SPKFTPLARATPSVVAQSYGSSGFVAPGTFVLAMVFLVAFVLYYFINWKYLSTLWGLS
                     "
     gene            complement(11537..12076)
                     /locus_tag="PK242_02545"
     CDS             complement(11537..12076)
                     /locus_tag="PK242_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HOZ92929.1"
                     /translation="MSAILPPSDRLWYKHPIDRVEGTWLAIALVWCLILFFMMVGWHI
                     WGKQNLSTETYRTTPDKFMAKAQAVVDKYTVRTETDEKIPVVAPPPGSDVYLLGRLWS
                     FWPILELEKGKTYRLHITAMDYNHGLSLQPANINIQLVPGFEHVVTVTPNQSGTYGLV
                     CNEYCGIGHHRMVSRLYVK"
     gene            complement(12095..12265)
                     /locus_tag="PK242_02550"
     CDS             complement(12095..12265)
                     /locus_tag="PK242_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92930.1"
                     /translation="MSPHDHDALNDPNAPIPWMQQLLDSPFLLLLLGVLIPMVLYNLW
                     GVVEILLLPVAK"
     gene            12614..13465
                     /locus_tag="PK242_02555"
     CDS             12614..13465
                     /locus_tag="PK242_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOZ92931.1"
                     /translation="MLSVITVSAFIRLSQSGVGCADWPVCYEQAAGHASAHPWLGLAR
                     ATHRVTASAMLLVVLGLLVATGRRTPDLRHERRLALGVLLLTLWLAGLGALMRGASAP
                     AVVLGNLLGGLATLALCGRLLVCGSDRTAPAPSLRRWAWWAMLLVFFQAAFGGLIDAR
                     HAASSCQHLSECADLARAAGWDWRVLSPWPAASAAGELAPQARGAWLHLLHRLGALLL
                     VPVLATLAWKALRQGFSIPAIGVFVLLVLELTLGSLLAATGFPLPLALAHNLVAALLL
                     TLLAGLA"
     gene            complement(13590..14882)
                     /locus_tag="PK242_02560"
     CDS             complement(13590..14882)
                     /locus_tag="PK242_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOZ92932.1"
                     /translation="MSQQWKFETQSVHAGYTPDPTTKSVAVPIYQTVAYAFDSAQHGA
                     DLFDLKVPGNIYTRIMNPTTDVLEKRVAALEGGIGALAVASGQAAILYALQTITEAGD
                     NIVSSTALYGGTYNLFAHTLPQYGIQTRFADHRDPASFAKLIDERTKAVYVETIGNPA
                     GNITDIASVAAIAHQHGVPLIVDNTVGTPYLIRPFEHGADIVVHSLTKYLGGHGTSLG
                     GAIVDSGKFPWAEHKARFKRLNEPDVSYHGVVYTEALGAAAYIARARVVPLRNTGAAL
                     SPFNAWQILQGIETLALRMDRICDNTLKVAQYLSQHPKVAWVSYAGLPDHEDHALAQH
                     YMQGRASGLFTFGVKAAQGGREAGARFLDALQLFTRLVNIGDTRSLATHPASTTHRQL
                     SPEELAKAGVREETVRLCIGIEHIDDLIADLDQALAKV"
     gene            15180..15452
                     /locus_tag="PK242_02565"
     CDS             15180..15452
                     /locus_tag="PK242_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HOZ92933.1"
                     /translation="MARMVHCIKLGTEAEGLDFPPYPGELGKRIWENVSKQAWQEWLR
                     HQTMLVNENRLNMADQRARDYLKRQMENHFFGAGADHVAGYVPPGS"
     gene            15639..17630
                     /gene="mnmC"
                     /locus_tag="PK242_02570"
     CDS             15639..17630
                     /gene="mnmC"
                     /locus_tag="PK242_02570"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HOZ92934.1"
                     /translation="MPDPIVHWRADGTPTSTRFDDVYRSAGSDGQAGLAQARQVFLRG
                     CGLLPEGDAAAAWAGAAHWAVLETGFGLGLNFLAAWQAWRQDPQRPARLFYSAVEAWP
                     PTADDLRRSAAPYPELRPLAEQLATQWHGLRPGVHRLGFDAEQVQLTLAVGEVQAALA
                     ELGGGHDSLWLDGFSPARNPEMWQLHTLKAAARLLRRGARAATWCVAAQVRRDLQTCG
                     FEVERVPGLPPKRHALRARYAPRWPTALERTAAPAVRPGRCAVIGAGLAGASVAWSLA
                     QRGWQVTVLDRAAQPARGASGLPAGVIAPHVSPDERPLSRLTQAGARATLARAALLLQ
                     GGIDFAASGVLERHAPGARRLPEPAQGSNAVAHDSLDADHPLTRQRAAAASVPLDPHR
                     RALWHTHAGWIRPPALVRAMLQAPGVVWRANTAVARIEPAADGWQLRGADGTLLAAAD
                     LVVVAAGFDSLALAGAPLPLHALRGQVAFGPMPGGAADAALPAFPVNGLGSLIGHLPG
                     ADGPWWITGSTFERARTLPDCTDADHASNRQRLAELLPAAAAALDAQWSDGRAQAWAA
                     VRATLPDRLPAVGAWPSQAPPDLMPKPAPAPYQQAPAAIDSEAPTARLPLQLCTGLGA
                     RGLTLAVLCGELLAAGLHGEPLPLARSLAQRLRAERFLS"
     gene            17648..18517
                     /locus_tag="PK242_02575"
     CDS             17648..18517
                     /locus_tag="PK242_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOZ92935.1"
                     /translation="MSARHLWLALVVVAIWGSNFVVIKLALAHLPPLLLATLRFALAA
                     VPLVLLVRRPPVSWGNLALYGLAIGVGQFGLLFFVMTRHISPGLASLVIQSQVFFTVG
                     LAMLLSGERVRPYQVVAALIAVAGIVLIASRTDGQTTPLGLALTLAAALSWAVGNIAS
                     RAAGPVDVLAYVAWSSLLAVPPLAVLSLTLEGWPRIAAGLVQADWATWAAVLWQSVGN
                     TIFGYGVWAWLLARYPAATVAPFSLLVPVFGMGASAALLAEPLPAWKLAAAALVMGGL
                     ALNLLWPRWRFNH"
     gene            complement(18818..19948)
                     /locus_tag="PK242_02580"
     CDS             complement(18818..19948)
                     /locus_tag="PK242_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOZ92936.1"
                     /translation="MQVQLKLTVAAALALVAGLASAQEQVVKIASVAPMSGGQAHYGK
                     DNGNGVTMAIEDLNKQNITIGGKKIKWEAVIEDDAADPKQGAAVAQKLCDMKVNGVVG
                     HLNSGTTIPASKIYHECGLPMVTGAATNPDLTKPGYKQIFRIIANDSALGAGMARYAK
                     DVLKLKSVAVIDDRTAYGQGLADIFANTAKSLGLQVVERQFTTDKATDFMAILTAIKA
                     KKPEGIFFGGMDPQAGPMLRQMDQLGMSKTPFMGGDGVCTVKLAELSAGAKSLANAAC
                     AEGGTSLQKMPGGMEWKKRYDAKFPNQYQVYSPYTYDATMLLADAMKRADSVDPKVYL
                     AKLAESNFKGITSTIAFESNGEVKNAAITISTYPGGKKTPLQ"
     gene            complement(20044..20445)
                     /locus_tag="PK242_02585"
     CDS             complement(20044..20445)
                     /locus_tag="PK242_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92937.1"
                     /translation="MNPDDEPSARPPPRGVPTLTEVVQAAAPPSEAPAPASLPQPDSP
                     VSPTSHVALEPAVPAQDPVDALLERLGPELERQIAEAVGRVLHEQMLGFNSRVRKAVA
                     DVVRDTVAASLLQAGGRSDVSENPENQPSDT"
     gene            complement(20449..20880)
                     /locus_tag="PK242_02590"
     CDS             complement(20449..20880)
                     /locus_tag="PK242_02590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HOZ92938.1"
                     /translation="MTAVAFHFNVPDKLGYACRLLRKAYAAGGPVGVVAPSGMLSELD
                     TRLWAFSALDFVPHCLADAPASMRAVTPIVLAEDCAQLPPATAVLVNLGPQVPDGFER
                     FGRVIELVTGDDDDRAQARQRWRHYAGRGLEIQRHDLAAGA"
     gene            complement(20907..22445)
                     /locus_tag="PK242_02595"
     CDS             complement(20907..22445)
                     /locus_tag="PK242_02595"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOZ92939.1"
                     /translation="MDFELQSLRLADAAARKSPALMVLVADGFAGGEDALSALIARTV
                     ASGDFKTEAGAQLFVYGAAGIVPERVVLAGIGDGSPARVRKAVGTAMGALRGGPAKAV
                     TLCFAQPAEADALRAAVLALADASYTYSATKSKPNDKKEPPRIGKAVLGVPEPGPLRE
                     ALQQAVAVARGVELAREWANRPGNHATPTLLADAARQLGKLPRIGCEVLGPKEVARLG
                     MGAFQAVAQGSRQELRFIVLRYDGAPRSQAPVVLVGKGITFDTGGISIKPAPEMDEMK
                     FDMSGAASVLGVFRALAELRPALNVVGLIPACENMPDGGAIKPGDVVTSMSGQTIEIL
                     NTDAEGRLVLCDALTYAERFKPCAVIDIATLTGACVIALGGVRSGMFASDDALAQALQ
                     SAGEQALDPCWRMPLDDEYGEALKSNFADVGNVGGRPAGAVTAAKFLQRFADKFSWAH
                     LDIAGTAWKGGSAKGATGRPVGLLVHYLLMQAGRPVKAGGEPTKRAKTKGQAGARAGK
                     GGKA"
     gene            22469..23566
                     /gene="lptF"
                     /locus_tag="PK242_02600"
     CDS             22469..23566
                     /gene="lptF"
                     /locus_tag="PK242_02600"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HOZ92940.1"
                     /translation="MLFDSSIRKELGRAFSATLVVLVTVVMTVLLIRTLGQASAGRVN
                     PSEVMLVMGYTVLGHLATILTLSLFVAITATLTRMYAASEMVVWFASGQGLAAFLRPV
                     LRFAWPVLLVVAVLALAVWPWANHQIRELRDRYQARGDIERVAPGRFIESAGGQRVFF
                     IDKDRADAETGHNIFIATNEHGKETVTTAQRGRLEVIKGERFVVLDHGHRLELDSATG
                     QTRVSEFAEYGNRISTRLVGPTANDSPTMKPTLTLLSQPTPRHQGELSWRIGLILAAL
                     NCAVFALAATRVNPRVGRSAGLVFALFAFTTYYNLLTFGEGWIARGRVGLVAYLLLLH
                     GGVLLLSLGWLLARHHGWGSAWRRRLNGGGA"
     gene            23563..24669
                     /gene="lptG"
                     /locus_tag="PK242_02605"
     CDS             23563..24669
                     /gene="lptG"
                     /locus_tag="PK242_02605"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOZ92941.1"
                     /translation="MNTIRHLIYREVLRAVGFVALAFLALFFFFDLVDALGQLERGAG
                     RGYHAGHALLNVALQVPSHLYELLPIAVLIGTIYVMAQLAASSQFTILRTSGLGPLRA
                     LGMLLVLALGFVALTFVCGDYVAPEANRAVQNLQARFLGDITVGRTGAWLKERRDDSQ
                     YAVNVGTMSADGTLNQVQIFQFSIADGRLLTMTKASRGHFSDQGWTLQAVEQRNFEQV
                     PQAEPRVQVQRLDEWVWPNALSQDTVAAALLNPNRMQTLDLFRYIRHLQSNQQSAQRY
                     EIEFWRKVFYPLSCLVMVVLALPFAYLHFRTGNITGYVFVGVLVGISFFLLNNVFGFI
                     GNLRNWEPWLAAAAPGVIYSVLSLAAFGWLVLRQ"
     gene            24672..25055
                     /locus_tag="PK242_02610"
     CDS             24672..25055
                     /locus_tag="PK242_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HOZ92942.1"
                     /translation="MQGPDRHGIVLFAHGSREAAWRAPIEAVAERIRQLAPGRPVACA
                     YLEHTAPDLPTAVDRLLAGGADAVTVWPMFLGVGRHAREDLPRLLQVLRTQHPQVRFS
                     LQPAISEHPEVLAAMAHAMLDTPSS"
     gene            25099..>25372
                     /locus_tag="PK242_02615"
     CDS             25099..>25372
                     /locus_tag="PK242_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ92943.1"
                     /translation="MNLHQFRFVQEAARRNLNLTEAAKALHTSQPGVSKAIIELEEEL
                     GIDIFTRHGKRLKRITEPGQHVLDSIAIILREVGNLKRIGEQYSAQD"
BASE COUNT         4155 a         8981 c         8475 g         3761 t
ORIGIN      
        1 ggccaggacg tgctgctgct ggacaacgac gacaccgtct ccatcggctc gcgcggcgtg
       61 tgctatgcca agcgcgcgct ggaggtgctc gaccgcatgg gctgcggcga caagttcgtc
      121 gacaagggcg tgagctggga cgtcggccgc accttcctgc gcgaaggcga agtgttcaac
      181 ttcaacctgc gccccgaggc cgaccaccac cgcccgggca tgatcaacct gcagcagtac
      241 tacctggagg agtacctggt cgagcgcgcg cgccagctgc cccggctgca gatgcgcttc
      301 aagaacaacg tcatctcggt cgtgccgggc gccgaccagg tgacggtgcg cgtcgagacc
      361 ccggacggcc cctactcgct caccaccgac tggctggtgg tggccgacgg cgcgcgcagc
      421 ccgatccgca ccatgatggg gctggacatc gagggcaaga tcttcatgga ccgcttcctc
      481 atcgccgacg tggtgatgaa ggccgacttt ccggccgaac gctggttctg gttcgacccg
      541 ccctttcacc ccaaccagag cgtgctgctg cacaagcagg ccgacaacgt ctggcgcatc
      601 gacttccagc tcggctggca ggccgacccg gtggaagagc gcaagcccga gaacgtgatc
      661 ccgcgcatca aggccatgct gggcgacgaa cgcgaattcg agctcgaatg ggtcagcatc
      721 tacaccttcc agtgccggcg catgaacagc ttcaaccacg ggcgcgtgct gttcgtcggc
      781 gacgccgcgc accaggtgtc gcccttcggc gcccgcggcg ccaacagcgg catccaggac
      841 accgacaacc tggtctggaa gctcaagctg gtgatggacg gcaaggcgcc gcccaccctg
      901 ctggacagct acaccgaaga gcgcggcttc gcggcggacg agaacatcat gaactcgacc
      961 cgttcgaccg acttcatcac acccaagagc aacaccagcc gcaccttccg caacgcggtg
     1021 ctggagctgg cagcgcagca cccgttcgcc cgggcgctgg tcaattcggg gcggctgtcg
     1081 gtgcccagct ggctggccag ctccagcttg aacacgcccg acaccgacgc tttccagggc
     1141 cagatgatcc ccggcgcgcc gatggccgac gcccccgtgc ggaccgccgg cggcgacggc
     1201 tggctgctgc accaggtcgg caaccgcttc caggcgctgc actacgtgga cgacgccgcc
     1261 gcgctcgaca cggacaccac ccgggcgctg agtcagctgg ccagccacgg catcgacgtc
     1321 gaacccatcg tggtggctcg ccggggccag gcaccggccg gcatccggac cctgatcgat
     1381 ggcaagggct gcatggccca gcgttacgac ctccaacccg gcaccaccta cctgctgcgc
     1441 cccgaccagc atgtggccgc gcgctggcgc accctgaacg ccgccagcgt caaggccgcc
     1501 ttggcacgcg ccaccggcaa cacctgagcc ccgaacccca ggagacacgc accatgcccc
     1561 tgaacaccca acccaacttc agcgaagccg gccgccgcta ctttcaggcc tactcgcctg
     1621 gcgacgactt ctacgaagcg ctgatcgaca cccaccgcga cctgagcgac gagcagagcg
     1681 ccatggtcaa cgcgcgcctg atcctgctgc tggccaacca catcggcgac atcggcatcc
     1741 tgcgccaggc catgcagatc gcgcgcgaag gcgtataacc gcgccacccc caaccattga
     1801 cccaagcaag gagactccca tgagcatgct gaaaggcgtc caccacgtgg cctatcgctg
     1861 caagaacgcc aaggaaaccg tcgagtggta caagaagtac ctggacgccg agttcgtgct
     1921 ggccatcgcc gaaaacgaag tgccttcgac caaggagccc gacccctaca tgcacatctt
     1981 catcgacctg ggcggcggca acatcctggc cttcttcgag ctgcccaccc gggcgccgat
     2041 gatcgcgccc tcggacgcca acaccccgtc ctggacccag cacctggcga tcaaggtcga
     2101 cagcctggac accatgatga aggccaaggc gcgactggaa gccgacggca tcgaggtgat
     2161 cggcccgacc gaccacacca tcttcaagtc gatctacttc cgcgacccat cgggccaccg
     2221 gctggagctg gccgtcaaca ccgccacgcc cgaaatggac aaggcgctcg acgacgtgaa
     2281 gtgggacatg ctggaggaat gggaccgcac caagaaggcc cccacccacg cccgctggat
     2341 gcacgacggc agcggccgcg tcggcccgca ctgagccgat ccacgatccg ccagaccccg
     2401 acgagagcgg cagcccgatc gctgccgcta tttttttcat agctactggc gcttgccacg
     2461 cgccgctcag ctcccctgcc ccgccgtggg caggaaggca tccgagaagc agaactgctc
     2521 gcccaggcca tgcgcgatga agtcgggcac cgccgcctcg accatccggg tggagccgca
     2581 ggcatagacc tcgtagccgg tcagatcggg gtgatcggcc agcagcgcct ggtgcacgaa
     2641 cccgcgccgg cccggccagg ggtcgtccgg cgccagatcg gacagcaccg gcacgtagcg
     2701 gaagttcgca tggcgctcct gccactgcgc cagcaggtcc tgcaggtaca gatcctcctg
     2761 cgaacgcacg ccccagtaca gctcgatcgg gcggcgaatg ccgcggcgga aggcgtcttc
     2821 cagaatgctc ttgatgggtg cgaacccggt cgccccggcg atcatcagga tcgggcgctc
     2881 gctgtcgcgc agggtgaagc ggccgatcgg cccttcgaag tcgaccacgt cgccttcctt
     2941 catggcttcg aacacgtggg tggtgaagcg cccgccctcg atcaaccgca cgtgcagctc
     3001 gatcaggttg tcggtcggca gcggaaaggg ttgcgccgga tcggccggcg cgttggcgaa
     3061 cgaaaacgcc cgcttggcac cgtcttccag gatgatgttg atgtactgcc cggcggtgaa
     3121 aggcagcgtc tggccggccg gcagcgccag gaccacccgc atcaccgtgg gtgaaatgcg
     3181 ctccagccgc tgcacccttg cctgcaggtg gcgcagttcg ctggcttcgt cgcgcgccag
     3241 cgtggccaca ccttcgacct cgatctccac gtcttccagc gccacggcgc agcacatcag
     3301 ggcctggccg cgcgcccgca tggccggggt cagcaccgcg tcctggtatg cacccaggtc
     3361 caccttgccg ttgagcacgg tgcagacaca cacgccgcag ccgcctgcgc ggcagtcgta
     3421 gggcaaggcg agctcggcgc gcaggccggc gtccagcagg gtttcgccgg cgcgcgccat
     3481 cacgcgcgcc gggccggggt ggatggtcaa ttgccgcggc gcatgggcgc tgcgccgcag
     3541 ggtcagccac ggcagcatca caccaaacgc ggtgctgccg accagcagca cccagaccgc
     3601 gcccatggca ccggactgaa tcagcggaaa caccgtcagc aggaaccagt cgaagctgag
     3661 cctgagcggc atggtgtgca gatcggccgg cccctggctg aacaccggca ccgccagcga
     3721 cagcaccagc agcgtgaccg acagggccac ggtgatcggc cggggcggat gcatggccgc
     3781 cttgggcacc cgctgcacat ggatccacat cactagcagc agcagcaggg acacgccgat
     3841 gtggatgaac accagcagcg agaacaggcg attgctgaca tgctcgggca ggatgaagtt
     3901 gcggatcagg cgccccttga agcccggcag ccagtccatc cactcgaagc tggcctgggt
     3961 gacgaactgg gccatccggt cccagggcag catgaagccg ttcaccccgg ccacgtaggt
     4021 cagccacagc agaccgaccc ctgtgaccca ggaaaaggcc cggaacccgc gcagccggtc
     4081 gaaggcaaag tagcgcaaca tgtgcagcag catggtcagc accatcgcat ccgaggcgta
     4141 gcggtgcagc gaacgcagca cgccgcccag tccccacagg ctgtgggtga tggtctccac
     4201 cgactggtag gcgccttcca cgctggtgtc gaagaaggca tacaggatca ggcccgtggc
     4261 gccgatgatc cagaacagga agaacgaaat cttgccgagg tggtagaagg ggttcatgcg
     4321 gtcaccgaag gcgcggttga acaccgcttc ggcgcccatg aagccggctc gcagcacgcg
     4381 ttgcacgatg ccgatcatgg cgcggccctc ctgtccagac cggccgtggg ttcggcccgc
     4441 atcgcccgca ccaccaccag ggcaaacagc aggccgccga cgatcgccag cagcccgccc
     4501 agccccatca gccccatgcc ggcgatctcg ccggtgctgc gcagcacctg ctcggcaccc
     4561 gccaccttgc gctgcacgcc atagccgccc gaccacatca ggccgacgat gtgcagcaac
     4621 tgccccaccc cgtacagcca gggctgggcg acggccagcc gcccccgggg cgcggcgtag
     4681 cccagctccg gcagcagccg gtacaccagg cccatcagcg ccagcgtgac gcccacgatg
     4741 cagccgtggt agtgggccgg aatcttgacg ttgctgccct gaatcgtcag cccgatcacg
     4801 ccgccggcga tgaacaacag catggacgcc agcaccgccg cgcgcaacgg gcgctgcacc
     4861 ggcgccagcc cgcgcatcgg cagcagcgcc agcagcaccg ccagcaccag cggcgcgatg
     4921 gccacgccac caccgaagcg catgccccag gtatgcatgt cgcgatgctc gacgctgctc
     4981 acgtcatgca tcagatacgc cagcggcgtg acgaacacgc tcaccagggc gatcaggaac
     5041 agcaccacca cgatgcgcgg actgagcggc acccgcccgc cgcaggactg caccagcacc
     5101 agccaggcca ccagcatcag cagcgtccag gtgaactgca gtgcgtgacc accgccccag
     5161 aacagaatct cgtaataagc cttggccggc agcgtggtcg gcaccaccac cagcgaccag
     5221 gcaaacgcca gcagggcgac gcccgcggcc accgcgccgg catgcagccc gaagcgcaag
     5281 gcgcccgtgc catcgaccgc cggaccgatc ggcaccgggc gcgccatggt cagcagcacc
     5341 agccacagcg cgccggcggc gaacacccac agcgcggcgc gaaacccggc gctgtccagc
     5401 atcgggatgt agttggccat gaccgcttcg ccggggtcca cgaacgcggc cagccccatg
     5461 cccagcgcgc ccagaccgca cagccacagc ggcgcgcgca gcagcaggcg gctgcgcggc
     5521 gagccgggcg gcctctggtt caggctccac agcaggccgg cgatggccac gaaccagacc
     5581 agcaccgaca ggtcgacgtg aaccaccagc gccacacgaa agaagtctcc ggccggcagc
     5641 caggcattga tgccgggcgt gcgcgccagc accagcagca ccgaaaacag accagatcca
     5701 agcagcgcgc ccagcgccag ccacagccag ccacgcgcca gcgagcgccc ggccgcggtg
     5761 acggccggca gctcgaagcg cgccggcgtc gaagccgtca cgcgctcggc gcgcacggtg
     5821 ggtgacggca ggcgcagatt cttcggggtc catgcggtat cggggttcat tgcagcgtga
     5881 tgccgccttt ctcggcgtca aagtccacca ccagcacctg tccgggcacc agattgacac
     5941 tggcctggcg gtggtaggtc accgcgccgg ggcgcacgtc gtccttgaag tgcacgtcga
     6001 tctgttgcgg gccggcggcc accaccaggc gctcgtagat cgacgccgag ccgtccttgg
     6061 acaggccgga tggcggcgcc acgcgctgca gcacgcgttg gccgccgaca tcgacctcca
     6121 tcgtcagcgg cgaacgctcg cgcgggcact gcagtggatc gcgcatgttg ggtggcagct
     6181 tggccagctc gtcgggactc aggcgccggc attcgcccac cggctgaccc agacgcgtga
     6241 cgctgatctt gatgaccgcc tgatcactgt cgattggccg gtaggtcggc cactgagaaa
     6301 acacgccgat cgccagcgcg aacaggccat acagcagcac ctggcccgcc cattgggcgg
     6361 cgcccggcgc tgcggtggag cggccttcac ggggttcagg tctggagggt gtagtcatcg
     6421 tggtggattc tcggaagatc ggaaagccca tgcacgcgca cgcgcaaacg ctcgaccgcg
     6481 gcgcgcagct cgaccgcatc gcccggaccc gccgccacca gctccaggcg ctcggcgggg
     6541 atgtgctggc gcaggtgcgg ctcgcgctgc cgctgcatgc gctccagcgt ccagcgctgg
     6601 cccaggcgaa actcgcagcc gcctgcccgg caggtcgcca ccacgacgcc ggccgcacca
     6661 tcgcgcagcg cgtattcgac gaaggacggc ggcagttggc cggtgcacag caggctgtag
     6721 gtgctgacgt cgcctgccgc ggcgccaccg gccgccgccc cgtggtcgca ggcaaacacc
     6781 accagcggtt gcgcggcggt cgccctgctc aggtcctgtc gcaggcgctg gcgcagggca
     6841 tcgacgggca gttgcggcat atcgatgccg gtcaccagtt gctcgatgcg gcgaaacggc
     6901 gtcgacgacg ggcaggcgcc ggcacagatg ccgcagccga cgcacaggtc cgcatcgacc
     6961 tgggccagct cgcgcccggc ccgccgatcg ggatggggca ccatggtgat cgcggcgtag
     7021 ggacagtcca ccaggcagcg ccggcagccg ctgcagtggt cggggttgac caccgccacc
     7081 gccgcgcgtg ccggctgcgg cagccagggc agcgccagca gcagcagcat ggcaccgatc
     7141 accagcgccc aggtgaaggc ggccgaggtc acgtcggtga gcggatgcag ccacagcagc
     7201 agccagtcga gctgcaacgc ggccggcacc cgccccagcg ccgcgggcgc atggctcacc
     7261 accggacggg ccagcgccag cagcgccagc acgcccagca ggcccagcat cagggcgcgc
     7321 ggcggcagca ccgtggcgtg ggccagccgc tggatgtgaa accacaggcc gaacagcagc
     7381 agcaccggca cgcccagatg gatgaagatg aagagcgaga acagacggtc gctgaccgcc
     7441 tgcgcggtaa ggaaattgcg cgccagcggg ctggtcagga aaggcagccg atcgagccac
     7501 tcggccgtcg ccagcgccga atactggctg agctgatccc agatcagcca gaacccgcca
     7561 atggcgctca cgaagcagaa cgccaccagg ggcacgccgg tcagccagtg aaagcggcgc
     7621 accccgcgct catgcccatg cagccactcg cgcacgatgt gcagcagcat gaccagcatc
     7681 agcacgtcag ccgcatagcg gtgcagcccc cgcaacagat ggcccagccc aagcggcagc
     7741 cgagccagcc cctcgaccga tgcgtaggcg cccgtcaccg aggtgtcgaa caggatgaac
     7801 agcacgatgc cgctggccac cagcagccac agcgccagaa accccagcgc gcccagatgc
     7861 cgcagcgggt tcaggccgga accgaatgca gagtcgaaca gccgctcgag cgcccgccag
     7921 ccactcagac cccatgaccg gatgctggct ggggcgtgct cggacatggc gctcgcaact
     7981 ggcgccgctg gcgccgctga ttgcgccact ccagccccag atagatcaag gtggaaagaa
     8041 agaacaacac gccgctgagc acgaccagga tcagcttgta gtcgtagcgg tattcgccgg
     8101 tttcttcgtc gtagaccgtg cacaggatgc gcacgcgctc gacgatgttc tcgaactgcc
     8161 ccagcgccgg cagcggctgg tcgtacagca gcagccggcg cagcggcaca cccaggcgat
     8221 cggcccgcac gatgtcgccc agcacctgac tgtggatgcg cccatcggca tcgaccacgg
     8281 tgacccccag cacatgatcg aagccggcgg gggtggccac ccagctgaag ccgaacgcac
     8341 gcgtcaaggc gtccacctgg tccgcacggg gactcagaaa ctcccagttg cgatgctgga
     8401 tgcggtgctg ggcggcaaac gcgcgcatcg cttccggcgc atcgaatggt tgattgaagc
     8461 cgatgctgac gacgttgaac tggtcctggc ccagcatcac gtccagtccc ttgacggatt
     8521 cgtacagcgt ccgtgtctgc gtcgggcaga tctcgaagca cccggtgtag atgaagctga
     8581 ccagcaaggg cttgccgcga aagtccgaca ggcgcaccgg cttgccgcgc cgatcgcgca
     8641 tctcgaagtc gggcaccgtg cgccccacgg cagcctcggc caggcgcacc gcttcgccca
     8701 catcgagcga gggcacctcg ccgggcgccc gcgccgccgc ccccggaccg tccgccagcg
     8761 ccggcccgcc agccagcgcc agccagatcg cccccacggc tacgcagccg cgcagcaggc
     8821 gtgtggccac gcgctgcgga cgagcctgct cagcgcaatg cggcatcgat cagcaccccg
     8881 gcgatgatgg cactcagttg catcatcgaa gcgaagaacg cgcccatcgc cctggcccgt
     8941 tcatgcgggg cacgcgccag cgctgcggtc ttgcgcagga agttcagccc gcccacgacc
     9001 gcccccagcc cgtaggccca gcccgccccg aacgccagcg gcagcagcga cacggccacc
     9061 agcacaaccg tgcagcggtg cacggtgcgc gccgcacgcg ccttgcccac caccaccggc
     9121 agcatgggta caccggcggc tgcgtactgt gcctcgttgg cgatcgccag gctccagaaa
     9181 tgcggcggcg tccacagcag cagcaccagc gccagcagca gacccagcgg ccccacctga
     9241 gggttggcgg ccgccgcgcc cgccagggcc gcaaagccgc cggcggcccc gccgatgacg
     9301 atgttccacg gcgtgcggcg cttcagaccc aaggtgtaga ccacggcgta ggtcagcgcg
     9361 cccagcagca cgaacagcgc cgccggcagg ttgacccacc acgcggcggc gccgaccgcc
     9421 ccggccagca tggcggccat cagcaccagc cagcccggcc cgcgctgcag ggcgccgctg
     9481 gcaaaggccc gcgtgcgggt acgtgccatc aggcggtcgc tgtcggcctc cacatactga
     9541 ttgaaggcgc cggcgctggc cgaagccacc agcaccgaca gcgccagcac cagagactgc
     9601 cacggcgttg cgccgccgtc gggcacgatc gccaggccgg cgatggcggt gagcatgatg
     9661 agcgtgccga tgcgcagctt gaacacactg agcaccagcc gccagccggc ggtggcgggc
     9721 cgtgtcgcca taaccatggc ggcggctgac gggtcgactc ggttcatgtg cgcctcctgc
     9781 tcgttggcga ccctcgtcac gccggacgag ggtcacgccc caccatcagc tcaggcccca
     9841 gagggtcgag aggtacttcc agttgatgaa gtagtacagc acgaaggcca ccaggaacac
     9901 catcgccagc acgaaggtgc cgggtgccac gaagccgctg gacccgtagg actgggccac
     9961 caccgaaggc gtggcacgcg ccaggggggt gaacttcggg ctgctggcgc cggcgtcgag
    10021 cttgcgtccc cacagcagcg aaccgacggt gatgtagatg tagatggcgc cgccggcgat
    10081 cgcggcgatg ccgccgatgc ccaccagcgc catcatcagg taggcggcac ccggccattc
    10141 ataggccagc gcggcaccct ggaacgccat gtcccagtgg cgacgcgaca cgcccagggt
    10201 acccgcaccc atcatcacca ggcagaagaa atacatcgag aagccgaaca ggtacggctg
    10261 ccactgcgcc agcttgggcg cgatcatctc gcgcttgaac agcaccggga tcaggtagta
    10321 ggtcagcgcc atgaaggtca gcgtggtgcc caccaccacc gtggcatgga agtgcccagg
    10381 cacatagatg gtgttgtgga tgatcaggtt gagctgctcg gtgcccatca tcacgccggt
    10441 gatgccgccc aggaagccga aaccgatgat cgagatgaac acgccggaga acgtcgggtt
    10501 gccccagggc gccttgcgca gccactcgaa caggcccttg gtgtagccct tctggcgctg
    10561 cgccacttcc atcgcgcccg gcaccgtcag accgtgcagc atcgaggcca gcaccgccag
    10621 gtacatgaag tagctggtgt tgacgacctt ccaggccgtg cccacgcccg ggtcggccag
    10681 caggtggtgg gcgctggcca gttgcaggaa caggatgtag agcaggaagg cggtgcggct
    10741 gacgcgctcg ctcatgggtt tggcgcccag ggcgatggcc gccaccagat accagaccga
    10801 cacgtgagcc gccacgttga tctgctgcga cgaatgcccc agcgcccacc acacggtgcg
    10861 gtagatcagc gggtcgacgt tcatcaggcc cagcgacatc agccaggccg ggacgatgat
    10921 catggcgccc gacagcagcg tgaagacggc aatgatggcg gccgtcaggg cgccgaaggt
    10981 caccagcgga atcgagcctt cataggtctt ttcgcgccgc gccaccacca gggtgccgaa
    11041 gaacacgaag cagccgatca gggcgccgac ggcaaacagg atgatgccca ggtagaagct
    11101 gggttcggcc atcatcggca catagctggt catcatgacg ctggagccgc cacggaacac
    11161 cgccacgttg ttgaggatgg agccgatcag catcagcgca aagccgagcc aggcgatctt
    11221 gggcgtggcg atgcgacagc gcagcaaggt cgatgaacag aaatgcagga tcgcgatctc
    11281 gaagaagatg atccagaaga tcagcatgtc cagaccgtgt gcggtcagca ccatgtagaa
    11341 ggtgtcggcc tcgagccagc gcacggccgg ccagcgcgtc agcacgacgc cgatcgccag
    11401 caggccgccg atcagcagcc agaccacgcc cacgaaggcg tgggccttga tcagcatctc
    11461 ggcctggcgc tcgaactgca gcccggagcg agggcaggta cggtaagtgg ttgccacggc
    11521 ggtcatccct gtctcctcat ttcacgtaca ggcggctgac catccgatgg tggccaatgc
    11581 cgcagtattc gttgcacacc agcccatagg tgccggattg gttgggcgtc accgtcacca
    11641 catgctcgaa gccgggcacc agctggatgt tgatgttggc cggctgcagc gacaggccgt
    11701 ggttgtagtc catggccgtg atgtgcaggc ggtaggtctt gcccttttcc agctccagga
    11761 tcggccagaa cgaccacagg cgccccagca gatagacgtc gctgccaggc ggcggagcca
    11821 ccaccgggat cttttcgtcg gtctcggtgc gcaccgtgta cttgtcgacc acggcctgtg
    11881 ccttggccat gaacttgtcg ggtgtggtgc gataggtttc ggtggagagg ttctgcttgc
    11941 cccagatgtg ccagccgacc atcatgaaaa acagaatcag acaccagacc agcgcaatgg
    12001 ccagccaggt gccctcgacg cggtcgatcg ggtgcttgta ccagagccga tctgacgggg
    12061 gaagaatggc gctcatgtga gtgttctcct ggagttactt ggccacgggc agaagcagga
    12121 tctcaaccac cccccacagg ttgtagagaa ccatcgggat cagcaccccc agcaacagca
    12181 acagaaaggg gctgtcgagc agttgctgca tccacgggat tggggcgttg gggtcgttca
    12241 gggcgtcatg gtcatgcggg ctcatgggtt ctcctccgga ggggtgggcg cgtgagcagc
    12301 ggttcctcag cgccggatcg cattctttac ccgaccaaac caatcggact tgccgcagat
    12361 caacaaatcc cggtcagacc ggccagtgcc ccctcgggca aaccccggct tgaccatctt
    12421 tccagtaggg gcgtgccacc gctaacatgc gctccacccg ctgccgccca cacccaggcg
    12481 cgcgccaccc atgcccaaca ccgctcctgt gccgaccccc cagcccctgc ccgacaccca
    12541 gcccgagcat ctcgatccgt tgcagcgccg ccgccgacgc atacgctcgc tggcagcgct
    12601 ggcctggctg ctgatgctga gcgtgatcac cgtcagcgcc ttcatccggc tgtcgcagtc
    12661 gggcgtggga tgcgcagact ggccggtctg ctacgaacag gcggccggac acgccagcgc
    12721 ccacccatgg ctggggctgg cgcgcgccac ccaccgcgtg acggccagcg caatgctgct
    12781 ggtggtgctg ggtctgctgg tggccacggg gcgacgcacg ccggacctgc gccacgagcg
    12841 ccggctggcg ctgggggtgc tgctgctgac cctgtggctg gccggcctgg gcgcgctgat
    12901 gcgcggcgcc agtgcgccgg cggtggtgct gggcaacctg ctgggtggcc tggccacgct
    12961 ggccctgtgt gggcggctgc tggtgtgtgg cagcgaccgc acggcgcccg caccgtccct
    13021 gcggcgctgg gcttggtggg cgatgctgct ggtgtttttt caggccgcct tcggcggcct
    13081 gatcgacgcc cggcacgccg ccagcagctg ccagcacctg tccgaatgcg ccgacctggc
    13141 gcgcgccgcc ggttgggact ggcgtgtgct gtcgccctgg cccgccgcca gcgccgcagg
    13201 cgagctggcg ccgcaggcgc gtggcgcctg gctgcacctg ctgcatcggc tcggcgccct
    13261 gctgctggtg cccgtgctgg cgacgctggc gtggaaggcc ctgcgccagg gcttcagcat
    13321 cccggccatc ggcgtgtttg tgctgctggt gctggaactc acgctgggca gcctgctggc
    13381 agccaccggt ttcccgctgc cgctggcgct ggcccacaac ctggtggccg ccctgctgct
    13441 cacgctgctg gccgggctgg cctgagcgaa ttgcacaccg ccacacgcaa caagctcaaa
    13501 agcgcccaga agcgaccgtc gatcatcgcc gcgtgctgga tgcaggctac gctctggcga
    13561 tgtcatgaat tccgagatct tcaataggat cagaccttgg ccagcgcctg atccaggtcg
    13621 gcgatcaggt cgtcgatgtg ctcgatgccg atgcacaggc gcaccgtctc ttccctgacg
    13681 ccggccttgg ccagctcctc gggcgagagc tggcggtgcg tggtgctggc cgggtgcgtg
    13741 gccagcgagc gcgtatcgcc gatgttgacc agtcgggtga acagctgcaa cgcgtccaga
    13801 aagcgcgcgc cggcctcgcg cccgccctgc gccgccttga cgccgaaggt gaacaggccc
    13861 gaggcgcgcc cctgcatata gtgctgggcc agtgcgtggt cttcgtggtc gggcagcccg
    13921 gcgtagctga cccaggcgac cttgggatgc tggctcaggt actgcgccac cttcagggtg
    13981 ttgtcgcaga tgcggtccat gcgcagcgcc agggtttcga tgccctgcag gatctgccag
    14041 gcgttgaacg gcgagagcgc ggcgccggtg ttgcgcagcg gcacgacccg cgcgcgggcg
    14101 atgtaggccg ccgcgcccag cgcctcggtg tagaccacgc cgtggtagct cacgtccggc
    14161 tcgttcaggc gcttgaagcg cgccttgtgc tcggcccagg ggaacttgcc gctgtcgacg
    14221 atggcgccgc ccagcgaggt gccgtggccg cccaggtact tggtcagcga gtgcaccacg
    14281 atgtcggcgc cgtgctcgaa cggccggatc aggtagggcg tgccgacggt gttgtcgacg
    14341 atcagcggca cgccgtgctg gtgggcgatg gcggccacgc tggcgatgtc ggtgatgttg
    14401 ccggccgggt tgccgatggt ttccacatac accgccttgg tgcgctcgtc gatcagcttg
    14461 gcaaagctgg ccgggtcgcg gtgatcggca aagcgggtct ggatgccgta ctgcggcaag
    14521 gtatgggcaa acaggttgta ggtgccgccg tacagcgcgg tgctgctgac gatgttgtcg
    14581 cccgcctcgg tgatggtctg cagcgcgtac aggatggccg cctggccgct ggccaccgcc
    14641 agcgcgccga tgccaccttc cagcgccgcc acgcgctttt cgagcacgtc ggtggtcggg
    14701 ttcatgatgc gggtgtagat gttgcccggc accttcagat cgaacaggtc ggcgccgtgc
    14761 tgcgcgctgt caaaggcgta ggccacggtc tggtagatcg gcaccgccac cgacttggtg
    14821 gtcggatccg gcgtgtagcc ggcatgaacg gactgggtct caaacttcca ttgctgcgac
    14881 atgagggaac tccttggaac accgaaattg ctacattgaa catagctgcc ggcgcttgct
    14941 ggatgcgcgc tacagccatt tttgttcaag aatccatggg gtctggccga gccaccgcca
    15001 gtggcccgac ccgagccgac agtctagcgc tgtcaggtac ctatagaagc cagaacaagg
    15061 cttgcggcaa ggccacgcgc gcggtgcggg cggcaaagct tgctacagtt ccgccaccgc
    15121 gcccgcgcca gcgggtcgtc cccaacgcga acaccgtcga acctcacaag gaccttccca
    15181 tggcacgcat ggttcattgc atcaagctcg gcaccgaggc cgagggcctg gactttccgc
    15241 cctacccggg cgagctgggc aagcgcatct gggaaaacgt cagcaagcag gcctggcagg
    15301 aatggctgcg ccaccagacc atgctggtca atgaaaaccg cctcaacatg gccgaccaga
    15361 gggcgcgcga ttacctcaag cgccagatgg aaaaccactt cttcggcgcc ggcgccgacc
    15421 acgtggccgg ctacgtgccg cccggcagct gaggcgggcg cgctccgcat cgggcccgaa
    15481 cgcgcgctgc ctggctgcag cagggttcgt gggtgacgcg ccgccgtctg cgtcggcatg
    15541 gccccggcag gccctcgggc ccgcaccaag ttcatggtca aatcagcctc tggcgcgctc
    15601 gcaacaagcg ccaacagcta ttgttttgat agtatttcat gcctgacccg atcgtccact
    15661 ggcgcgccga cggcaccccc accagcacgc gcttcgacga tgtgtaccgc agcgccggca
    15721 gcgacggcca ggctgggctg gcgcaggcac ggcaggtgtt cctgcgcggc tgcggcctgc
    15781 tgcccgaggg cgacgccgcc gcagcctggg ccggtgccgc gcactgggcg gtgctggaaa
    15841 ccgggttcgg cctgggcctg aactttctgg ccgcctggca ggcctggcga caggatccgc
    15901 agcggcctgc gcggctgttc tacagcgcgg tcgaagcctg gccgcccacc gccgacgacc
    15961 tgcggcgcag cgccgcgccc tatcccgagc tgcggccgct ggccgagcag ctggcgacgc
    16021 agtggcatgg cctgcggccg ggcgtgcatc ggctgggctt cgacgccgag caggtgcagc
    16081 tgacgctggc ggtaggtgag gtccaggcag cgctggccga actcggcggc ggtcacgaca
    16141 gcctgtggct ggacggcttc agtcccgccc gcaaccccga gatgtggcag ctgcacaccc
    16201 tcaaggcggc ggcccggctg ctgcggcgcg gcgcccgcgc cgccacctgg tgcgtggccg
    16261 cccaggtgcg ccgggacctg cagacctgcg gcttcgaggt cgaacgcgtg cccggcctgc
    16321 cacccaagcg ccacgccctg cgcgcccgct acgccccgcg ctggcccacc gccctggaac
    16381 gcaccgccgc gccggccgtg cgcccgggcc gctgcgccgt catcggcgcc ggcctggccg
    16441 gggcctcggt ggcctggagc ctggcgcagc gcggctggca ggtgacggtg ctggatcgcg
    16501 ccgcgcagcc ggcccgcggt gccagcggcc tgccggccgg agtgatcgcg ccccatgtct
    16561 cgcccgacga gcgcccgctc tcgcgcctga cgcaggccgg tgcgcgcgcc acgctggcac
    16621 gcgccgcgct gctgctgcaa ggcggcatcg acttcgccgc cagcggcgtg ctggagcgcc
    16681 acgcgcccgg cgcgcggcgc ctgcccgagc cggcgcaagg ctccaacgcc gtcgcccacg
    16741 acagcctgga cgcggaccac ccgctgacgc gccagcgcgc cgccgccgcc agcgtaccgc
    16801 tcgacccaca ccggcgcgcc ctgtggcaca cacacgccgg ctggatccgc ccaccggcgc
    16861 tggtgcgcgc catgctgcag gcgcccggcg ttgtgtggcg tgccaacacg gcggtggcgc
    16921 gcatcgagcc cgccgccgac ggctggcagc tgcgcggcgc cgacggcacc ctgctggccg
    16981 cggccgatct ggtggtggtg gccgccggct tcgattcgct ggcgctggcc ggcgccccgc
    17041 tgccgctgca cgccttgcgc ggccaagtcg ccttcgggcc gatgccgggc ggcgcggccg
    17101 acgcggcgct gccagccttt ccggtcaacg ggctgggcag cctgatcggc cacctgccgg
    17161 gcgccgacgg cccctggtgg atcaccggct ctaccttcga gcgcgcccgg acgctgcccg
    17221 actgcaccga cgccgaccac gccagcaacc gccagcgcct ggccgagctg ctgccggcgg
    17281 ccgccgccgc gctggacgcg caatggtccg acggccgggc ccaagcctgg gcggcggtgc
    17341 gcgccaccct gcccgaccgc ctgccggcgg tcggcgcctg gccgagccaa gcaccgccgg
    17401 atttgatgcc aaaaccagct ccagcgccct accagcaagc gccagcagct attgattcag
    17461 aagcacccac ggcgcgcctg ccgctgcagc tgtgcaccgg cctgggtgcg cgcggcctga
    17521 ccctggcggt gctgtgcggc gagctgctgg cggccgggct gcacggcgag ccgctgccgc
    17581 tggcgcgctc gctggcgcaa cgcctgcgtg ccgaacggtt cctgtcatga gtccttcccg
    17641 taccgcgctg agcgcgcgcc acctgtggct ggccctggtg gtggtcgcca tctggggcag
    17701 caacttcgtc gtcatcaagc tggcgctggc gcacctgccg ccgctgctgc tggccacgct
    17761 gcgctttgcc ctggccgccg tgccgctggt gctgctggtg cggcgcccgc cggtgtcctg
    17821 gggcaacctg gcgctgtacg ggctggccat cggcgtcggc cagttcgggc tgctgttctt
    17881 cgtcatgacg cgccacatct cacccgggct ggcttcgctg gtgatccagt cgcaggtgtt
    17941 cttcacggtc gggctggcca tgctgctgag cggcgagcgg gtgcgcccct accaggtggt
    18001 ggcggcgctg atcgccgtcg ccggcatcgt gctgatcgcc agccgtaccg acggccagac
    18061 cacgccactg ggactggcgc tgacgctggc cgccgccctg tcctgggcgg tcggcaacat
    18121 tgccagccgc gccgccggcc cggtcgacgt cctggcctac gtggcctggt ccagcctgct
    18181 ggcggtgcca ccgctggccg tgctcagcct gaccctggag ggctggccac gcatcgccgc
    18241 cggattggtg caggcggact gggccacctg ggccgccgtg ctgtggcagt cggtcggcaa
    18301 caccatcttc ggctacggcg tctgggcctg gctgctggca cgctacccgg cggcgacggt
    18361 ggcgccgttt tcgctgctgg tgccggtgtt cggcatgggc gcttcggcgg cgctgctggc
    18421 cgagccgctg cctgcctgga agctggccgc cgccgcgctg gtgatgggcg ggctggcgct
    18481 caacctgctg tggccacgct ggcgcttcaa ccattgaacc tagaagcggc agttgaccgc
    18541 tggtcaactt cgggcagacc gcatcgatgc ggggtctggc ggctgcgatg gccttcacct
    18601 cctgggtgag cacgctacgc acccacccgc accgcgctcg gcgcgccatg cggcgttgct
    18661 cctgcttgcg ggcacgagcc cgcggcggcg tcgcgtcttg cctggcacgc cgatcacggc
    18721 acgggcgggc gcgtccaacc gccgtttcta ggttgaacct aggatgaaaa aagccgcccg
    18781 aaggcggctt ttcaggacga ccgggtcgtc acgccgatca ctgcagcggc gttttcttgc
    18841 cacccgggta ggtggaaatg gtgatggcgg cgttcttgac ttcgccgttg gattcgaagg
    18901 cgatggtcga ggtgatgccc ttgaagttgg actcggccag cttggccagg tagaccttcg
    18961 gatcgaccga gtcggcgcgc ttcatggcgt cggccagcag catggtggcg tcgtaggtgt
    19021 aggggctgta gacctgatac tggttcggga acttggcgtc gtagcgcttt ttccattcca
    19081 tgccgccggg catcttctgc agcgaggtgc cgccctcggc gcaggccgcg ttggccagcg
    19141 acttggcgcc agcggacagc tcggccagct tcacggtgca cacgccgtcg ccacccatga
    19201 acggggtctt gctcatgccc agctgatcca tctggcgcag catcgggccg gcctgcgggt
    19261 ccatgccgcc aaagaagatg ccttcgggct tcttggcctt gatggcggtc aggatggcca
    19321 tgaagtcggt cgccttgtcg gtggtgaatt ggcgctcgac cacctgcaga cccagcgact
    19381 tggcggtgtt ggcgaagatg tcggccagac cctggccgta ggcggtgcgg tcatcgatga
    19441 cggccaccga cttcagcttg agcacgtcct tggcatagcg cgccatgccg gcgcccaggg
    19501 cgctgtcgtt ggcgatgatg cggaagatct gcttgtagcc cggcttggtc aggtcggggt
    19561 tggtggcggc gccggtgacc atgggcaggc cgcactcgtg atagatcttg gacgccggga
    19621 tggtggtgcc cgagttcagg tgaccgacca cgccgttgac cttcatgtcg cacagcttct
    19681 gcgccaccgc agcgccctgc ttgggatcgg cggcgtcgtc ttcgatcacg gcttcccact
    19741 tgatcttctt gccgccgatg gtgatgttct gcttgttcag gtcttcgatc gccatcgtca
    19801 cgccgttgcc gttgtccttg ccatagtgcg cctggccgcc cgacatgggc gccacgctgg
    19861 cgatcttgac gacctgttcc tgggcgctgg ccaggccggc aacgagggcc agagcagcgg
    19921 ccacggtcag tttcagttga acctgcatga tgggaaatac ctccgatgaa atgaagccca
    19981 cgactgggct tctggggcgc gaacgatacg aggtttttgg gctaccggcc actagggaac
    20041 acgctaggtg tcggaaggct gattctcagg gttttcactg acatccgaac gcccgccggc
    20101 ctgcagaagg ctggccgcca ccgtgtcgcg caccacatcg gccaccgcct tgcgcactcg
    20161 gctgttgaaa cccagcatct gttcgtgcag cacacggccg accgcctcgg cgatctggcg
    20221 ctccagttcg ggccccagcc gctccagcag cgcatccacc gggtcctgcg ccggcacggc
    20281 cggctccagg gccacatgcg aggtcggcga gaccggcgag tccggctgcg gcaggctcgc
    20341 gggcgcaggc gcttcagacg gcggcgccgc ggcctgcacc acctcggtca gggtcggcac
    20401 cccacgcggc ggcggtcgcg ccgacggttc gtcgtccggg ttcatggctt acgctccggc
    20461 ggccagatca tggcgttgaa tctccagtcc gcgcccggcg tagtggcgcc agcgctggcg
    20521 cgcctgcgcg cgatcgtcat cgtcacccgt gaccagctcg atcacacgcc cgaaacgctc
    20581 gaagccgtcc ggcacctgag ggcccaggtt gaccagcacc gcggtcgccg gcggcagctg
    20641 ggcgcaatct tcggccagca cgatcggcgt caccgcccgc atcgatgccg gcgcgtcggc
    20701 caggcaatgg ggcacgaaat cgagcgcgga aaaggcccac aaccgggtgt cgagctcgct
    20761 cagcatcccg ctcggcgcca ccacccccac cgggccaccg gctgcgtacg ccttgcgcag
    20821 caagcggcag gcgtagccca gcttgtccgg cacgttgaag tgaaacgcca ctgcggtcat
    20881 cgcggccacc gggtcggcag catgtgtcag gccttgccgc cttttccggc cctggcgccg
    20941 gcctgaccct tggttttggc acgcttggtc ggctcgccac cggccttgac cggccgtccc
    21001 gcctgcatca gcaggtaatg caccagcagc cccaccgggc ggccggtggc gcccttggcg
    21061 ctgccgccct tccaggcggt accggcgatg tccaggtgag cccagctgaa cttgtcggca
    21121 aagcgctgca ggaacttggc cgccgtcact gcgccggccg ggcgcccgcc cacgttgccc
    21181 acatcggcaa agttgctctt gagcgcctcg ccatattcgt cgtccagcgg catgcgccag
    21241 caagggtcga gcgcctgctc gccagcgctc tgcagggcct gggccagcgc atcgtcgctg
    21301 gcaaacatgc cgctgcgcac gccgcccagg gcgatcacgc aggccccggt cagggtggcg
    21361 atgtcgatca ccgcacatgg cttgaagcgc tcggcatagg tcagcgcgtc gcacagcacc
    21421 agccgtcctt cggcgtcggt gttcaggatt tcgatggtct gaccgctcat gctggtgacc
    21481 acatcgcccg gcttgatggc gccaccgtcg ggcatgttct cgcaggccgg gatcaggccc
    21541 accacgttga gcgccggcct cagctcggcc agcgcgcgga acacgcccag cacgctggcg
    21601 gcgccactca tgtcgaattt catctcgtcc atctcgggcg ccggcttgat cgagatgccg
    21661 ccggtgtcga aggtgatgcc cttgcccacc agcaccaccg gcgcctgcga ccggggcgcg
    21721 ccgtcgtagc gcagcacgat gaagcgcaac tcctgccgcg acccctgcgc caccgcctgg
    21781 aaggcgccca tgcccaggcg cgccacctcc ttcggaccca gcacctcgca gccgatgcgt
    21841 ggcagcttgc ccagttgccg ggccgcgtcc gccagcaggg tcggcgtggc atggttgcct
    21901 ggccggttgg cccattcgcg cgccagttcg acgccgcgcg ccaccgccac cgcctgctgc
    21961 aacgcctcgc gcagcgggcc cggttccggc acccccagca cggccttacc gatgcgcggc
    22021 ggctccttct tgtcattcgg tttgctcttg gtggcgctgt aggtgtagct ggcgtcggcc
    22081 agggccagca cggcggcgcg cagcgcatcg gcttcggcgg gctgggcaaa gcacagcgtg
    22141 acggccttgg ccgggccacc gcgcagcgcg cccatggccg tgcccaccgc cttgcgcacc
    22201 cgggccggac tgccgtcgcc gatgccggcc agcaccacgc gctcgggcac gatgcctgcc
    22261 gcgccataga cgaacagctg ggcgccggcc tcggtcttga agtcgccgct ggccacagtg
    22321 cgcgcgatca gcgccgacag cgcatcttcg ccaccggcga agccgtcggc caccagcacc
    22381 atcagggcgg gcgacttgcg ggcggccgcg tcggccagac gaagggattg aagctcgaag
    22441 tccataattg ccgttttcct ttcgactgat gttattcgat tcctccattc gcaaggagct
    22501 cggccgggcc ttcagtgcca cgctggtggt gctggtcacg gtggtgatga cggtgctgct
    22561 gatccgcacg ctgggccagg cctcggccgg ccgcgtcaac ccctcggaag tgatgctggt
    22621 gatgggctac accgtgctcg ggcacctggc caccatcctc accctgagcc tgttcgtcgc
    22681 catcaccgcc acgctgacgc gcatgtacgc ggccagcgag atggtggtgt ggttcgccag
    22741 cggccagggc ctggcggcgt ttctcaggcc cgtgctgcgc tttgcctggc cggtgctgct
    22801 ggtggtggcg gtgctggcgc tggcggtgtg gccctgggcc aatcaccaga ttcgcgagct
    22861 gcgcgaccgc taccaggcgc gtggcgacat cgagcgggtg gcgccggggc gcttcatcga
    22921 gtcggccggg gggcagcgcg tgttcttcat cgacaaggac cgtgccgacg ccgagaccgg
    22981 ccacaacatc ttcatcgcca ccaacgaaca cggcaaggaa accgtgacca cggcgcagcg
    23041 cgggcgcctg gaggtgatca agggggaacg cttcgtggtg ctcgaccacg gccaccggct
    23101 ggagctcgac agcgccactg gccagacgcg ggtgagcgag ttcgccgaat acggcaaccg
    23161 catcagcacg cgcctggtgg gaccgaccgc caacgattcg cccaccatga agccgacgct
    23221 gaccctgctg agccagccca cgccccgcca tcagggtgag ttgtcgtggc gcatcgggct
    23281 gatcctggcg gcgctcaact gcgcggtgtt cgcgctcgcc gccacgcgcg tgaacccgcg
    23341 cgtggggcgc agcgccggac tggtgttcgc gctgttcgcc ttcaccacct actacaacct
    23401 gctgacgttc ggcgaaggct ggatcgcccg gggccgcgtc gggctggtgg cctatctgct
    23461 gctgctgcac ggcggcgtgc tgctgctgtc gcttggctgg ctgctggcgc gccaccacgg
    23521 ctggggcagc gcctggcgcc gccggctgaa cgggggtggc gcgtgaacac catccgccat
    23581 ctgatctacc gggaggtgct gcgtgccgtc ggcttcgtgg cgctggcgtt tctggcgctg
    23641 ttcttcttct tcgacctggt cgacgccctg ggtcagcttg agcgcggcgc cggacgcggc
    23701 taccacgcag gccacgcgct gctgaacgtg gcgctgcagg tgcccagcca cctgtacgag
    23761 ctgctgccga tcgcggtgct gatcggcacc atctacgtga tggcgcagct ggccgccagc
    23821 tcgcagttca ccatcctgcg caccagcggt ctggggccgt tgcgggcgct gggcatgctg
    23881 ctggtgctgg cgctgggctt cgtggcgctg accttcgtgt gcggcgacta cgtggccccg
    23941 gaggccaacc gcgcggtgca gaacctgcag gcgcgctttc tgggcgacat cacggtgggg
    24001 cgcaccggcg cctggctgaa ggagcggcgc gacgacagcc agtacgcggt caacgtgggc
    24061 accatgtcgg ccgacggcac gctcaaccaa gtgcagatct ttcagttcag catcgccgac
    24121 ggacgcttgc tgaccatgac caaggccagc cgtggccatt tcagcgatca gggttggacg
    24181 ctgcaggctg tggagcagcg caacttcgag caggtgccgc aggccgaacc gcgggtgcaa
    24241 gtgcagcgtc tggacgaatg ggtctggccc aacgcgctgt cgcaggacac cgtggccgcc
    24301 gccctgctca accccaaccg catgcagacg ctggacctgt tccgctacat ccgccacctg
    24361 cagagcaacc agcagagcgc gcagcgctac gagatcgagt tctggcgcaa ggtgttctac
    24421 ccgctcagct gcctggtgat ggtggtgctg gcgctgccgt tcgcctacct gcactttcgc
    24481 accggcaaca tcaccggcta cgtgttcgtc ggcgtgctgg tgggtatcag cttcttcctg
    24541 ctcaacaacg tgttcggctt cattggcaat ctgcgcaact gggagccctg gctggcggcg
    24601 gcggcgcctg gcgtgattta ttcggtgctg tcgctggccg ccttcggctg gctggtgctg
    24661 cggcaatagc catgcaaggg cccgatcgcc acggcatcgt gctgttcgcg cacggctcgc
    24721 gcgaagcggc atggcgcgcg ccgatcgaag ccgtggccga gcgcatccgc cagcttgcgc
    24781 cgggccgccc ggtggcctgc gcctatctgg agcacaccgc gcccgatctg cccaccgccg
    24841 tggaccggtt gctggccggc ggtgccgacg ccgtcaccgt ctggcccatg ttcctgggcg
    24901 tcggccgcca tgcgcgcgaa gacctgccgc ggttgctgca ggtgctgcgc acgcagcacc
    24961 cgcaggtgcg tttcagcctg cagccggcga tctcggagca ccccgaggtg ctggccgcca
    25021 tggcccatgc gatgctggat acgccctcgt cttagagcga ttcagccata atgatggagt
    25081 tctatattcg gaatctccat gaatcttcat cagttccgtt tcgttcagga ggccgcccga
    25141 cgcaacctga acctgaccga ggccgccaag gcgctgcaca cctcgcagcc gggcgtgtcc
    25201 aaggccatca tcgagctgga agaagagctc ggcatcgaca tcttcacgcg ccacggcaag
    25261 cgcctcaagc gcatcaccga gccgggccag catgtgctgg acagcatcgc catcatcctg
    25321 cgcgaggtcg gcaacctcaa gcgcatcggc gagcagtaca gcgcccagga ca
//
LOCUS       DAOOOG010000016        25245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_1840_length_25245_cov_22.518857, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000016 DAOOOG010000000
VERSION     DAOOOG010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 25245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25245
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1840_length_25245_cov_22.518857"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PK242_02620"
     CDS             <1..1115
                     /locus_tag="PK242_02620"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOZ92944.1"
                     /translation="EKLAEHKTFIQSLPYMDRLDYVSMMCNEHAYCLAIEKLLGIEVP
                     IRAQYIRVMFAEITRLLNHLMWLGSHGHDCGASTMLLYTFREREDLFDMYEAVSGARM
                     HAAYFRPGGVYRDLPDSMALYKSSKIRSSKSLDAINANRQGSLLDFIDDFTKRFPVLM
                     DEYETLLTDNRIWKQRTVGIGVVTPERALNLSLTGPMLRGSGIAWDLRKTQPYDVYAA
                     LDFDIPVGKTGDSYDRYLVRVQEMRESNRIIKQCVDWLRANPGPVIVDNHKVAPPPRE
                     RMKSGMEDLIHHFKLFSEGMHVPEGEAYAAVEHPKGEFGIYLISDGANKPYRMKIRAP
                     GFAHLSAFDELTKGHMLADAVAIIGTMDVVFGEIDR"
     gene            1112..1630
                     /gene="nuoE"
                     /locus_tag="PK242_02625"
     CDS             1112..1630
                     /gene="nuoE"
                     /locus_tag="PK242_02625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOZ92945.1"
                     /translation="MSSAVAPSTATPFSQATLARFAREVAKYPADQKQSAVMACLAIV
                     QQEQGWVSPDSERAVAAYLGMPVMAVHEVTTFYNMYNQQPVGRFKLNVCTNAPCMFAG
                     GPQALEHLCETLGVQPGETTADGLFTVQACECLGACGDAPVMLVNDRHMCSFMSNDKL
                     DQLVNGLRHAED"
     gene            1634..2980
                     /gene="nuoF"
                     /locus_tag="PK242_02630"
     CDS             1634..2980
                     /gene="nuoF"
                     /locus_tag="PK242_02630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOZ92946.1"
                     /translation="MARVQDILAQFQARGLETCFHGRHIHPQIYAGLDGTNWGLKDYE
                     ARGGYQALRKVLGQDGGPGMTQDEVIAELKASSLRGRGGAGFPTGLKWSFMPRNFPGQ
                     KYLVCNSDEGEPGTFKDRDILMHNPHAVIEGMAIAAYAMGASVGYNYIHGEIFRVYER
                     FEAAIEQAREAGYLGQGIMGSGFDFQLHAHHGFGAYICGEETALLESLEGKKGQPRFK
                     PPFPASFGLYGKPTTINNTETFAATPWIIRHGGQAYLECGKPNNGGTKIFSIQGDVAR
                     PGNYEIPLGTPFAKLLELAGGVRGGKTLKAVIPGGSSSPVLPADIIMQCTMDYDSIAK
                     AGSMLGSGAVIVMDESRDMVETLRRLSYFYMHESCGQCTPCREGTGWLWRMVDRIQEG
                     QGRPEDLDKLDNVAENIMGRTICALGDAAAMPVRAMIKHFRPEFAAKIDAAQKLTS"
     gene            3004..5118
                     /gene="nuoG"
                     /locus_tag="PK242_02635"
     CDS             3004..5118
                     /gene="nuoG"
                     /locus_tag="PK242_02635"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HOZ92947.1"
                     /translation="MVELEIDGKKVEVPEGSMVMHAAEKADIYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPMPACATPVTQGMVVRTKSDKAIKAQKSVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGASGSRYGDEKRVVAPKNVGPLISMKEMTRCIHCTRCVRFGQEI
                     AGAMELGMSNRGEHSEIETFVGQSVDSELSGNMIDICPVGALTSKPFRFSARTWELSR
                     RKSISPHDSTGANLVVQVKNHQVMRVVPFENEAVNECWIADRDRFSYEALNGEQRLTK
                     PMLKQDGVWREVDWQTALEHVANGLNEIRASHGAGRIGALVSPHSTLEELHLAASLVR
                     GLGSENIDHRLRHSEFPAPEGVRWLGTSVASLSELQSVLVVGSNLRKDHPLFAQRIRQ
                     AARHGCAVAAITSRALLKTDTAWAMPLRAVQAVEAGDWAQALADVATVLAADKGVPAP
                     AAGQATDSARALAGSLQSGERKAILLGNAAAHHAQASVLLALCQWIGEQTGATVGWLT
                     EAANTVGAQVVHAVPGKGGLNAAQMLAGDVDALLLLACEPGADSAPLQRQGCGLVVTL
                     SPFKANLDISDVLLPIAPFTETSGTFINAEGRVQSFHAVVRPLGDTRPGWKVLRVLAD
                     LLQVVQPAFETSQEVLAHALGGSVSEFVPAARLSNRTTTAIQIRAGAVDVSPVGIYQL
                     DGIVRRAPSLQQTADALEGASA"
     gene            5115..6191
                     /gene="nuoH"
                     /locus_tag="PK242_02640"
     CDS             5115..6191
                     /gene="nuoH"
                     /locus_tag="PK242_02640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOZ92948.1"
                     /translation="MIDAIYNVGLQLFAGSWWSGIAWPVVWSLIKIVVLLLPLMGAVA
                     YLTLWERRLLGFMQARLGPNRVGPAGLLQPIADAVKLLTKEIIRPTAAAKGLFFIGPV
                     MAIMPALAAWVVIPFGPEAALANINAGLLLVLAITSIEVYGVIIAGWASNSKYPILGA
                     LRASAQMVSYEIALGFCFLVVLMVSGSMNLTDIVMGQAKGMAADKGLGFLSWNWLPLL
                     PIFMVYLISVVAEVNRHPFDMAEGEAEIVAGHMVEYSGMGFATFFLAEYASMWLVSIL
                     AVLMFLGGWLPPIGALDFIPGWIWLGIKTFIVVSMFIWIRATFPRYRYDQIMRLGWKI
                     FIPVTLIWLIVVGLWMHSPWNIWH"
     gene            6206..6715
                     /gene="nuoI"
                     /locus_tag="PK242_02645"
     CDS             6206..6715
                     /gene="nuoI"
                     /locus_tag="PK242_02645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOZ92949.1"
                     /translation="MASVTASPFSLRDFLKSFVLVELFKGMALTGRYAFRRKVTIQYP
                     EEKTPLSPRFRGLHALRRYDNGEERCIACKLCEAVCPAMAITIEAGERADGSRRTTRY
                     DIDLTKCIFCGFCEESCPVDSIVETHILEYHGEKRGDLYFTKDMLLAVGDRYESEIAA
                     AKAADARYR"
     gene            6750..7421
                     /locus_tag="PK242_02650"
     CDS             6750..7421
                     /locus_tag="PK242_02650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOZ92950.1"
                     /translation="MDVKTGFFYVFALVMLFSAFRVITARNPVHAVLYLMLTFSQAAG
                     IWLLLKAEFLGIALVLVYLGAVMVLFLFVVMMLDIHIDTVRQGFWRHVPLALAIGALI
                     TLEMAVVLFGGFRLSEAPQVPETMTNAAGQVVQYSNTKVLGTLLYTKYLYPVEIAAVL
                     LLIAMVAAIALTLRERKDTKKVDPSLQVRVKASDRLQVLKLAPTQAPLPVEPPAAAIV
                     EEVKP"
     gene            7418..7726
                     /gene="nuoK"
                     /locus_tag="PK242_02655"
     CDS             7418..7726
                     /gene="nuoK"
                     /locus_tag="PK242_02655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOZ92951.1"
                     /translation="MTLTLAHFLTLGAILFSLAVVGIFLNRKNIIVLLMAIELMLLAI
                     NMNFVAFSHYLGDMHGQVFVFFILTVAAAEAAIGLAILMLLFRAKSSINVDEINSLKG
                     "
     gene            7734..9755
                     /gene="nuoL"
                     /locus_tag="PK242_02660"
     CDS             7734..9755
                     /gene="nuoL"
                     /locus_tag="PK242_02660"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOZ92952.1"
                     /translation="MSQTLSAATLLAVPLAPLAGSLIAGILGTAFGGNRIGRTASHTV
                     TILGVLIAFVLSALTLQSVIAGARFEGTLYEWMVVGGLKMEVGFLIDSLTATMMCVVT
                     FVSLMVHIYTIGYMADDDGYNRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSY
                     LLIGFWFNRPTAIFANLKAFLVNRVGDFGFILGIGLIAAYAGSLNYAEVFGKAGQLAN
                     LGFPGTDWLLVTVICICLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAG
                     IFMVARMSPLFELSDAALNFVLVIGAITALFMGFLGIIQNDIKRVVAYSTLSQLGYMT
                     VALGASAYSVAVFHLMTHAFFKALLFLAAGSVIIGLHHNQDIRWMGGVRKYMPITWIT
                     FLLGSLALIGTPFFSGFYSKDAIIEAVHASKLPGAGFAYFAVLAGVFVTAFYSFRLYF
                     LVFHGQERYDQNPDAHHHDDHHGHGHDAKPHETPWVVWLPLVLLAIPSVVIGAITLMP
                     MLFGDFFRLNDVIHVDAARHPAMAVLAEKIHGWVPMALHGFVAPPFWLALAGVACAYY
                     MYMVNPALPAAIQRAFRPVYSLLENKYYLDWFNENVVARGARLLGTGLWKAGDRALID
                     GLLVNGSWRLVGWVSGMVRWIQSGYLYHYALVMILGIFVLMTYFVWLNK"
     gene            9774..11249
                     /locus_tag="PK242_02665"
     CDS             9774..11249
                     /locus_tag="PK242_02665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOZ92953.1"
                     /translation="MGWLSLAIWTPIVFGAVLLALGRDSQAQLVRWIALIGSVVSLLI
                     TLPLYTGFRLGTAEMQFVEKMPWISRFNVNYYLGVDGISLWFVLLTAFITVIVVIAGW
                     QVIQRKVNQYMAAFLILSGLMVGVFAALDGVLFYVFFEATLIPMYLIIGIWGGPRKIY
                     AAFKFFLYTLLGSMLMLVAFIYLYAVSGGSFDVLTWHKLPLSATAQTLLFFAMFAAFA
                     VKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKLGAYGFLRFSMPILPDASREWAWLM
                     IALSLIAVIYIGLVALVQQDMKKLVAYSSVAHMGFVTMGFFIFNDLGVTGGLVQMIAH
                     GFVSGAMFLCIGVLYDRVHSREISSYGGVVNSMPRFGAFALLFFMANCGLPGTAGFIG
                     EWMVILATVRANFWLGLLLATALITGAAYSLWMFKRVYFGAVANDDVRAMPDIDGREF
                     LVLALLAIAVLWMGLYPKPFTDVMDASVVNLLQHVANSKLN"
     gene            11260..12747
                     /gene="nuoN"
                     /locus_tag="PK242_02670"
     CDS             11260..12747
                     /gene="nuoN"
                     /locus_tag="PK242_02670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HOZ92954.1"
                     /translation="MIDRFSWMAVYPEIVLLVMACVITLVDLLDRNPRRLAAYVLSIL
                     TLLVLAVLTGTYARGGQTWYGFEGMVVSDPMTNWLKCFAALAVAVSLVYARTYVTGRG
                     MLRGAEWYVLSLLSLLGMYVLISANNFLLVYLGLECMTLSGYALVALRRDHVTSIEAA
                     MKYFVLGAMASGFLLYGLSMLYGATGTLDIGGVFKAVASGQIKHQVLVFGVVFVVAGL
                     AFKFGAVPFHMWLPDVYEGAPTAVTLMIGGAPKLAAFAMAVRLLVEGLLPLAIDWQQM
                     LAVLAVASLLIGNLAAIAQTNLKRMLAYSTIAQMGFVLLGLMAGVVNHSALAAANAYS
                     SAMFYVVAYVLTTLVSFGIILLLSREGFESEEIVDFAGLNQRSPLYAGLMAICLFSLA
                     GIPPMVGFQAKLAVLQALVSTGLPSYIGLAVFAVMMSLVGAFYYLRVVKVMYFDEPLS
                     SAPIQAPFDMRAVLSVNGLLLLVLGIVPGSLMALCAKAVVQMLAT"
     gene            12750..13055
                     /locus_tag="PK242_02675"
     CDS             12750..13055
                     /locus_tag="PK242_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HOZ92955.1"
                     /translation="MSQNLSIWLLILLALVAANLPFVNHRLMAVFPLRTAAKGLAWRL
                     LELLLWYGFVIGVGMAIERAQGQNQPQGWEFYAVTAALFLTLAFPGFVYRYLVRRGR"
     gene            13055..13648
                     /locus_tag="PK242_02680"
     CDS             13055..13648
                     /locus_tag="PK242_02680"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOZ92956.1"
                     /translation="MASDLRETRVGGQELFKGDFLHAMRDRVRLPDGQLTQREYIVHP
                     GAVMIVPWLQGPDGSLQLVLERQYRYPVQQTIVEFPAGKLDPGEAPLVCAQRELREET
                     GYTAREWARAGLLHPVVSYSTEFIEVWFARGLQAGERRLDEGEFLEVFTASPEQLYAW
                     CRDGEITDAKTLIGALWLQNVASGAWSLNWQPADPAA"
     gene            13656..14132
                     /locus_tag="PK242_02685"
     CDS             13656..14132
                     /locus_tag="PK242_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HOZ92957.1"
                     /translation="MKVLNLQCAHGHAFEGWFASEDDFQDQRTRQLVECPLCGDVEVT
                     KLPSAPRLSLSGASAADTASSASATGPTAAAASVANLPPAALQAAWMHLVRQVMARTE
                     DVGERFAEEARRMHYGETEDRGIRGQATPAETEALLEEGIAVMPLPLPPGFKGTLQ"
     gene            complement(14248..14967)
                     /gene="fnr"
                     /locus_tag="PK242_02690"
     CDS             complement(14248..14967)
                     /gene="fnr"
                     /locus_tag="PK242_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HOZ92958.1"
                     /translation="MTPQAIKVACSNCNLRELCMPMGLSDTEMGKLDDLVATRRKVKR
                     GTSLFANGDRFTALYAIRTGFFKTCVATADGRDQVTGFQMAGEVIGLDGIVGDRHTCD
                     AVALEDAEVCVMPYDRIEELSREVQALQSHVHKIMSREIVREHGVMLLLGSMRAEERL
                     AAFLLNLVQRLHARGFSQSELVLRMTREEIGSYLGLKLETVSRTFSKFAEEGIVEVKQ
                     RHVRILNTEALRAIVNAPSCS"
     gene            15161..16543
                     /gene="hemN"
                     /locus_tag="PK242_02695"
     CDS             15161..16543
                     /gene="hemN"
                     /locus_tag="PK242_02695"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOZ92959.1"
                     /translation="MQAITPELLTKFDVPGPRYTSYPTADRFVEAFGEPDYLRALDQR
                     RTGPVAGARPLSLYVHIPFCESLCYYCACNKIITKHHDRSAPYLRYLSKEVDLHVQHL
                     GAGQSVSQLHLGGGTPTFFSDDELGELMSMLRRNFNLVAGGEYSIEVDPRTVDATRLQ
                     TLARLGFNRLSFGVQDFDPLVQKAVHRIQPAEQVFALVDAARRIGFESINVDLIYGLP
                     RQTPESFDRTLAQVAQLRPDRIALYGYAHLPERFKPQRRIVVVDLPGAANKVAMLARA
                     LAAFDAAGYDYIGMDHFALPDDALAVAKRQGRLHRNFQGYSTQPDCDLIGLGVSAIGK
                     IGATYSQNAKALEDYQDLLDQGHLPVVRGLAVSRDDLLRRAVIMALMCQGEVLYESIE
                     LAHLIRFQDYFASELETLRGMQAQGLVRLTDTGIEVTPMGWFFVRGVAMVFDKYLQAD
                     RTRARFSKII"
     gene            16543..17226
                     /locus_tag="PK242_02700"
     CDS             16543..17226
                     /locus_tag="PK242_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOZ92960.1"
                     /translation="MQSSLAVTALVMGLAGGPHCIAMCGAACAGIGQAAAPRSTQALA
                     LFQLGRLTGYAALGALAAATMQGLGWLTVQSAALRPVWTMVHVAAVLLGLSLLAFARQ
                     PVWLEAGARRVWARVRTATQSLGLVAPLGLGVAWALLPCGLLYSALMVAALAGSVVGG
                     AAVMALFALGSGLWLGLGPWLLLRLGRSGRGAWSMRVAGLALAATSAWGLWMGLVHDQ
                     APWCVVPTP"
     gene            complement(17314..18138)
                     /locus_tag="PK242_02705"
     CDS             complement(17314..18138)
                     /locus_tag="PK242_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOZ92961.1"
                     /translation="MPIEPLLFIELAALGLATGFLAGLLGIGGGMIMVPFISTMLVGR
                     EVAPGLAVKMAIATSMATIIFTSMSSVRAHHKKGAVRWDIVKRLAPGIVLGGLVASLG
                     VFALLKGAALYFVFAGFVAFSAVQMYLDKKPAASRQLPGTTGQLAAGSVIGFLSGLVG
                     AGGGFVSVPFMTWCNVAIHNAVATSAALGFPIALSNVLGYIVAGQGLQGLPAYSFGYL
                     WLPALVVIASCSVLMAPVGARAAHALPVKKLKRAFAGVLFVLALYMLNKGIGALTG"
     gene            complement(18470..18805)
                     /locus_tag="PK242_02710"
     CDS             complement(18470..18805)
                     /locus_tag="PK242_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOZ92962.1"
                     /translation="MKQVEVRIMGQAYLLAAPEGGEQRLQQAVQRVDAAMCKIRDAGR
                     IKARDRIAVLAALNLAFELTDAPRPAGAATTAPTDTPAGADEPASQAALGALLARLDQ
                     ALGNDGQLL"
     gene            complement(18802..19065)
                     /locus_tag="PK242_02715"
     CDS             complement(18802..19065)
                     /locus_tag="PK242_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HOZ92963.1"
                     /translation="MTTPPLIEQLSERVERLLVRHEELQRTNDLLAQQVQALTHERDN
                     LRSRLMAARARIDSLIDRLPATAGVLSGEATPDTPPAGKEATR"
     regulatory      19125..19393
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            19488..21320
                     /locus_tag="PK242_02720"
     CDS             19488..21320
                     /locus_tag="PK242_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ92964.1"
                     /translation="MSLRLQSFRPAALPLALACAFCAHAAQDTTVAQLAAPSLPDTVV
                     TATRVAQPLTDVLADVTVLGDEQIRASGAVSIGDLLARQPGLELARNGGLGSATSVYL
                     RGAETRFTAVYIDGIRIDSQATGGAPWEAIALGQVERIEIVRGPAAAVYGSDAIAGVV
                     QIFTRKGEGPFAPYVGVGLGSHRSGKLEAGFSGRNGALDYALGAARELSRGYNSMPAG
                     NPDRDGYRQSSASGRLGWNINDAHRLELSGTYTDIDAQYDGFLPGLDDHGLNRLGTLG
                     AAWSAQWSKRYSTRLSVSESDQRYETVPSPYLTKTRLRNYLLQNEWREGGHLITATLE
                     RREDHLVNAPIAQGRHQNAIALGYGFSGGPHTLQVNLRHDRDSEFGGQTTGGVAYGYG
                     FAPGWRATVAAGTAFRVPTLYQRFSEYGQPALQPEKGRNVELGLHWAQGDSRFGVSVW
                     RNRVSNLIVFGAPGPCAGAFGCYENAGRARLQGVTISGAHQLGGVNLSAALDLQQPRN
                     TLTDKLLPRRAQRLLKLSADIRVGGWTLGAEWQASSHRWDNAANTRRLAGYGIVNLYA
                     STTLARDWQVLARLDNLGDRDYQLAHGYATPGRSLFVGLRWTPR"
     gene            21344..21925
                     /locus_tag="PK242_02725"
     CDS             21344..21925
                     /locus_tag="PK242_02725"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOZ92965.1"
                     /translation="MDVARSELILGGQKSGKSRRAESLAAAWLAAAPTHRAVLIATAG
                     AHDAEMRARIARHQRERAARVPGLVTHEAPHELAAAIAQAGGAETLRIVDCLTLWLTQ
                     RLMPLPGHESHGPAALDGQAPAAIIIEALRSCPGPIVLVGNEIGLGVIPLGAEVRAFV
                     DALGQLNQAVAARCQRVTLMAAGLPLTLKDAAP"
     gene            21922..22788
                     /locus_tag="PK242_02730"
     CDS             21922..22788
                     /locus_tag="PK242_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOZ92966.1"
                     /translation="MSRWLLALLAWVWVGLVSAQELRDDAGQPLRLDAAPRRIVSLLP
                     SLTETVCALGACERLVGVDRYSNWPASVQTLPQVGGGLDPNIEAVVALKPDLVLIGTS
                     SRAALRLRALGLNVLALEPRTRAELRRVTQRLGTALRLDGADALARRIDDGVAAAAAA
                     LPVAARGQRVYYEITPAPHAAGQGSFIHELLLALGLVNVIDDPALGPFPRLNPELVVR
                     ADPDLIMVSQGGAADMARRPGWAGLRALREGRVCEFNAAQRDILVRPGPRMPEAARLM
                     ADCAARRPLQNR"
     gene            22824..23927
                     /locus_tag="PK242_02735"
     CDS             22824..23927
                     /locus_tag="PK242_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOZ92967.1"
                     /translation="MNLHTRAMDAPAHAATAALPPQGGARVRWLALALLALALLGLGL
                     GASVGSTGFDSLLRAWHDAVAWQIVWDIRLPRTLGAWLAGALLGLAGAVAQGLFRNPL
                     ADPFLLGSASGAALAVALTFAALGAGGLVAGGHGVALLQAASPWLARLGFTGAAFVGA
                     LGGVLLALVLAQGVQQTLRLLLAGVVVGYVLGAVRELVQLAQPDVLQAMQGFSLGVTG
                     FVGWGACAVMAAVLAPLLPLAWALARGLDALSLGEATARSLGLPLGLLRALLIGVLAL
                     ATGTAVAQTGLIGFVGLVAPHLARALARCTHGPLLLLASLMGGLLLTAADIAARLLIA
                     PQELPVGVLTAVLGGLYLLWLMHRRGRAGGALQ"
     gene            24008..24733
                     /locus_tag="PK242_02740"
     CDS             24008..24733
                     /locus_tag="PK242_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ92968.1"
                     /translation="MALELPQGRWCAVVGPNGAGKSTLLRVLAGLLPCQGQISLWGRP
                     LHDWPARARARQLAWLGQQEGGGEDLLAWDIAMLGRLPHRPWLADASAHDQAIVADAL
                     RSMQVWALRGRRLGELSGGERQRVLLARLLAVQAPLLLMDEPLAHLDPPHQADWLAVV
                     RAQVAAGGTVVSVLHELNMALAADDIVVLRAGRLLHHGPAACAHTHAALRAAFDGRIS
                     IRQLERQWVALPQPDRSEPDHAD"
     gene            24723..>25245
                     /locus_tag="PK242_02745"
     CDS             24723..>25245
                     /locus_tag="PK242_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOZ92969.1"
                     /translation="MQIESPPTEQRYERPAGERRGLVIVHTGDGKGKSTAAFGLALRA
                     FGRQHVHGKQVVIYQFMKVPTARFGEHRAFEQLGLPVHGLGDGFSWKSQDLEHSAQLA
                     REGWQKARAAILDGQHFLVVLDEITYPLIYGWLPLGDVLHTLRQRPREVHVCLTGRRC
                     PPELIELADTVTEM"
BASE COUNT         3780 a         8478 c         8596 g         4391 t
ORIGIN      
        1 ccgagaagct ggccgagcac aagaccttca tccagtcgct gccctacatg gaccgtctcg
       61 actacgtgtc catgatgtgc aacgagcacg cctactgtct ggcgatcgaa aagctgctcg
      121 gcatcgaagt gccgatccgg gcgcagtaca tccgcgtcat gttcgccgag atcacgcgcc
      181 tgctcaacca cctgatgtgg ctgggttcgc acggccacga ctgcggcgcc tcgaccatgc
      241 tgctctacac cttccgtgag cgggaggacc tgttcgacat gtacgaagcg gtgtcgggcg
      301 cgcgcatgca cgcggcgtat ttccgccccg gtggcgtgta ccgtgacctg cccgacagca
      361 tggcgctgta caagtccagc aagatccgca gcagcaagtc gctggacgcg atcaacgcca
      421 accgccaggg ttcgctgctg gacttcatcg acgacttcac caagcgtttc ccggtcctga
      481 tggacgaata cgagacgctg ctcaccgaca accgcatctg gaagcagcgc accgtgggca
      541 tcggcgtggt gacgcccgag cgcgcactca acctgagcct gaccggcccc atgctgcgcg
      601 gctccggcat cgcgtgggac ctgcgcaaga cgcagcccta cgacgtctac gctgccctgg
      661 atttcgacat cccggtgggc aagacgggcg acagctacga ccgctacctg gtgcgcgtgc
      721 aggaaatgcg cgaatccaac cgcatcatca aacagtgtgt cgactggctg cgtgccaacc
      781 ctggtccggt gatcgtggac aaccacaagg tggcgccgcc gccgcgggaa cgcatgaagt
      841 cgggcatgga ggacctgatc caccacttca agctgttttc cgaaggcatg cacgtgccgg
      901 agggcgaagc ctacgcggcg gtcgaacacc ccaagggcga gttcggcatc tacctcatca
      961 gcgatggcgc caacaagccc tatcggatga agatccgagc gcccggtttt gcccatctgt
     1021 ccgcgttcga tgagctgacc aagggtcaca tgctggccga cgccgtggcc atcatcggga
     1081 cgatggacgt cgtgtttgga gagatcgatc gatgagcagt gcagttgccc cttccactgc
     1141 gacaccgttt tcgcaagcca cgctggcgcg ttttgcgcgc gaggtcgcca agtaccctgc
     1201 cgaccagaag cagtcggccg tgatggcctg cctggccatc gtgcagcagg agcagggctg
     1261 ggtcagcccc gacagcgaac gggcggtggc ggcctacctg ggcatgccgg tgatggcggt
     1321 gcatgaggtc acgaccttct acaacatgta caaccagcag ccggtcggcc gcttcaagct
     1381 gaacgtgtgc accaacgcgc cgtgcatgtt cgccggcggg ccgcaggcgc tggagcacct
     1441 gtgcgagacg ctcggcgtgc agccgggtga aaccaccgcc gatggcctgt tcacggtgca
     1501 ggcctgcgag tgcctgggtg cctgtggtga cgcgccggtg atgctggtca acgaccgcca
     1561 catgtgcagc ttcatgtcca acgacaagct ggatcagctg gtcaatggcc tgcgccacgc
     1621 ggaggactga ttcatggctc gtgtgcaaga catcctcgcg cagttccagg ccaggggcct
     1681 ggagacctgc ttccacggtc gccacatcca tccacagatt tatgccgggc tggacggcac
     1741 caactggggc ctgaaggact acgaagcacg cggcggctac caggcgctgc gcaaggtgct
     1801 gggccaggac ggtgggcccg gcatgacgca ggacgaggtg atcgccgagc tgaaggcgtc
     1861 gagcctgcgt ggccgcggcg gcgcggggtt cccgaccggc ctcaagtgga gcttcatgcc
     1921 gcgcaacttc ccgggccaaa agtacctggt gtgcaactcc gacgagggcg aaccgggtac
     1981 cttcaaggac cgcgacatcc tgatgcacaa cccgcatgcg gtgatcgagg gcatggcgat
     2041 cgccgcctac gccatgggtg ccagcgtggg ctacaactac atccacggcg agatcttccg
     2101 ggtctacgaa cgctttgaag ccgccatcga gcaggcgcgc gaggccggct acctgggcca
     2161 gggcatcatg ggcagcggct tcgatttcca gttgcacgcg caccacggct tcggtgccta
     2221 catctgcggc gaggaaaccg cgctgctgga gtcgctcgag ggcaagaagg gtcagccgcg
     2281 cttcaagccg ccgttcccgg ccagtttcgg tctgtacggc aagccgacca ccatcaacaa
     2341 cacggaaacc tttgcggcca cgccctggat cattcgccac ggcggtcagg cctacctgga
     2401 gtgcggcaaa cccaacaacg gcggcaccaa gatcttctcc atccagggcg acgtggcgcg
     2461 ccccggcaac tacgaaattc cgctcggcac gccgtttgcc aagctgctcg aactggccgg
     2521 tggcgtgcgc ggcggcaaga cgctgaaggc ggtcatcccg ggcggctcgt cctcgccggt
     2581 gctgccggcc gacatcatca tgcaatgcac gatggactac gactccatcg ccaaggccgg
     2641 ctccatgctg ggctcgggtg ccgtgatcgt gatggacgag tcgcgcgaca tggtcgagac
     2701 cctgcgccgc ctgtcgtact tctacatgca tgagtcctgc ggccagtgca cgccctgccg
     2761 cgaaggcacc ggctggctgt ggcgcatggt cgatcggatc caggaaggcc aggggcgccc
     2821 cgaggacctg gacaagctcg acaacgtggc cgagaacatc atgggccgca ccatctgcgc
     2881 gctgggcgat gccgccgcga tgccggtgcg cgccatgatc aagcacttcc ggcccgaatt
     2941 cgcggccaag atcgacgccg cacagaagct gacgagctga agcgaacgga atcggacaag
     3001 cccatggttg aactcgaaat agacggcaag aaggtcgagg tgcctgaagg cagcatggtg
     3061 atgcatgcgg ccgagaaggc ggacatatac atcccgcact tctgctatca caagaagctg
     3121 tccattgccg ccaactgccg catgtgcctg gtggacgtcg aaaaggctcc caagcccatg
     3181 ccggcttgcg ccacccccgt gacgcagggc atggtggtgc gtaccaagag cgacaaggcg
     3241 atcaaggcgc agaagtcggt gatggagttc ctgctcatca atcacccgct cgactgcccg
     3301 atctgcgacc agggcggcga atgccagctg caggacctgg cggtcggcta tggggcctcc
     3361 ggctcgcgct acggcgacga aaagcgcgtg gtcgcgccca agaatgtcgg gccgctcatt
     3421 tccatgaagg agatgacgcg ctgcattcac tgcacccgct gcgtccgctt cggccaggag
     3481 atcgccggcg cgatggaatt gggcatgtcc aaccggggcg aacactccga gatcgagacc
     3541 ttcgtcgggc agtcggtcga ttccgaactg tcgggcaaca tgatcgatat ctgtccggta
     3601 ggcgcactca ccagcaaacc gtttcgcttc agtgcccgta cctgggagct gtcgcgccgc
     3661 aagagcatca gcccgcacga ctcgaccggc gccaacctgg tggtgcaggt caagaaccat
     3721 caggtgatgc gcgtcgtccc gttcgagaac gaggccgtca acgaatgctg gatcgccgat
     3781 cgcgaccgct tctcctacga agcgctgaac ggcgagcaac gcctgaccaa gcccatgctc
     3841 aagcaggacg gcgtgtggcg ggaggtcgac tggcagaccg cgctggagca cgtggccaac
     3901 ggcttgaacg agatccgtgc cagccacggc gcgggccgca tcggtgccct ggtcagtccg
     3961 cacagcacgc ttgaagaact gcatctggcg gcttcgctgg tgcgcgggtt gggcagcgaa
     4021 aacatcgacc atcgcctgcg ccacagcgaa ttccccgcgc ccgagggggt gcgctggctg
     4081 ggtacttcgg tcgcctccct gtccgagttg caatcggtgc tggtggtggg gtcgaacctg
     4141 cgcaaggacc atccgctgtt cgcgcaaaga attcggcagg cagcccgcca tggctgtgcg
     4201 gtggcagcta tcacttcaag agcattgttg aagaccgaca cggcctgggc catgccgctg
     4261 cgcgccgtgc aggcggtcga agcgggcgac tgggcgcagg ccctggccga tgtggcgacg
     4321 gtgctggcgg ccgacaaggg cgtgccggcc ccggcggctg gtcaggccac cgattcggcc
     4381 cgcgcgctgg ccggcagcct gcagtcgggt gagcgcaagg cgatcttgct gggcaatgcg
     4441 gcggcacacc acgctcaggc ttcggtgttg ctggccttgt gccaatggat cggcgagcag
     4501 accggcgcca ccgtgggttg gctcaccgag gcggccaaca cggtgggcgc acaggtggtg
     4561 cacgccgtac cgggcaaggg cggcctgaac gccgcccaga tgctggcggg cgatgtggac
     4621 gcgctgctgc tgctggcctg cgagccaggc gccgattcgg cgcccctgca gcggcagggc
     4681 tgcggcctgg tggtcacgct cagtccgttc aaggccaacc tggacatcag cgacgtgctg
     4741 ctgccgatcg ccccgttcac cgaaacctcg ggcaccttca tcaatgccga gggcagggtg
     4801 cagagcttcc atgccgtggt gcggccgctg ggcgatacgc gcccgggctg gaaggtgctg
     4861 cgcgtgctgg ccgacctgct gcaggtggtg caaccggcct tcgagacctc gcaggaggtg
     4921 ctggcccatg cgctgggcgg cagcgtgtcc gagttcgtgc cggccgcacg cctgagcaac
     4981 cgcacgacga ccgcaatcca gatccgcgcg ggcgctgtgg acgtctcccc tgtgggcatc
     5041 taccagctcg acggcatcgt gcgccgggcg ccgtcgctgc agcagaccgc cgacgccctg
     5101 gaaggagcaa gcgcatgatc gacgccatct acaacgtcgg cctgcagctg tttgccggca
     5161 gctggtggag cggcatcgcc tggccggtgg tctggagcct gatcaagatc gtcgtgctgc
     5221 tgctgccgct gatgggcgcg gtggcgtacc tgaccctgtg ggagcggcgg ctgctgggtt
     5281 tcatgcaggc gcgtctgggg cccaatcggg tcggtccggc cggcttgctg caacccatcg
     5341 ccgacgccgt caagctgctg accaaggaaa tcattcgtcc cacggcagcg gccaaggggc
     5401 tgttcttcat cggtcccgtc atggccatca tgccggcgct ggccgcctgg gtggtgatcc
     5461 cgttcggccc cgaggctgcg ctggccaaca tcaatgccgg tctgctgctg gtgctggcga
     5521 tcacctcgat cgaggtctat ggcgtcatca tcgccggctg ggcttccaac tccaaatacc
     5581 cgatcctggg tgccctgcgc gcatcggcgc agatggtcag ctacgagatc gcgctgggct
     5641 tctgcttcct ggtggtgctg atggtgtcgg gcagcatgaa cctgaccgac atcgtgatgg
     5701 ggcaggccaa gggcatggcg gccgacaagg gcctcggctt cctgtcctgg aactggctgc
     5761 cgctgttgcc gatcttcatg gtctacctga tctcggtggt ggccgaggtc aaccgccatc
     5821 cgttcgacat ggccgagggt gaggccgaga ttgtggccgg gcacatggtc gagtattccg
     5881 gcatgggctt tgccaccttc ttcctggccg agtacgccag catgtggctg gtgtcgatcc
     5941 tcgccgtgtt gatgttcctg ggcggctggt tgcctccgat cggtgcgctg gacttcattc
     6001 cgggatggat ctggctgggc atcaagacct tcatcgtggt gtcgatgttc atctggatcc
     6061 gtgccacctt cccacgttac cgctacgacc agatcatgcg cctgggctgg aagatcttca
     6121 tcccggtgac gctcatctgg ctgatcgtcg tgggcctgtg gatgcacagc ccctggaaca
     6181 tctggcactg accgcagggt tcgtcatggc ttccgtcact gcttcaccgt tttcgctccg
     6241 cgatttcctc aagagcttcg tgctcgtgga attgttcaag ggcatggccc tgaccgggcg
     6301 ctacgccttc cggcgcaagg tcaccatcca gtaccccgag gaaaaaaccc cgctgtcgcc
     6361 gcgctttcgc ggcctgcatg cgctgcgccg ctacgacaat ggcgaagagc gctgcatcgc
     6421 ctgcaagctg tgcgaggccg tatgcccggc catggccatc accatcgagg ccggcgagcg
     6481 cgccgacggt tcgcgtcgca ccacccgcta cgacatcgat ctgaccaagt gcatcttctg
     6541 cggcttctgc gaagagagct gtccggtcga ctcgatcgtc gaaacccata tcctcgagta
     6601 ccacggcgag aagcggggtg acctgtattt caccaaggac atgctgctgg ccgtgggcga
     6661 tcgctacgaa tctgaaatcg ccgccgccaa ggcggcagat gccaggtacc gctgacccgc
     6721 gctggctggc ccaccactag acaagaccca tggacgtcaa gaccggcttt ttctacgtgt
     6781 tcgccctggt gatgctgttt tcggcattcc gggtgatcac cgcgcgcaac ccggtgcacg
     6841 cggtgctgta cctgatgctc accttctcgc aggcggccgg catctggctg ctgctgaagg
     6901 cggagttcct cggcatcgcg ctggtgttgg tctacctcgg cgcggtgatg gtgttgttcc
     6961 tgttcgtggt gatgatgctg gacatccaca tcgacaccgt gcggcagggc ttctggcgcc
     7021 atgtgccgct ggcgctggcg atcggtgcct tgatcacgct cgaaatggcg gtggtgctgt
     7081 tcggtggctt ccgcctcagc gaagcgcccc aggtgcccga gaccatgacc aatgccgcgg
     7141 ggcaggtggt gcagtactcc aacaccaagg tgctgggtac gctgctctac accaagtacc
     7201 tgtatccggt ggaaatcgcc gcggtcctgt tgctgatcgc catggtggcg gccatcgcgt
     7261 tgaccctgcg tgagcgcaag gacaccaaga aggtcgatcc cagcctgcag gtcagggtca
     7321 aggcgagcga ccgcctgcag gtgctcaagc tggcgcccac gcaggcgccg ctgccggtcg
     7381 agccgcccgc cgcggccatc gtggaggagg tcaaaccatg acgctgacgc tggcccactt
     7441 tctgacgctg ggcgccatcc tgttttctct cgccgtggtg gggatctttc tcaaccgcaa
     7501 gaacatcatc gtgctgctca tggccatcga actgatgctg ctggccatca acatgaattt
     7561 cgtggcgttt tcgcattacc tgggcgatat gcacggtcag gtgttcgtgt tcttcatcct
     7621 gaccgtggcg gcggccgaag cggccatcgg cctggcgatc ctgatgctgc tgttccgcgc
     7681 caagtccagc atcaacgtgg acgagatcaa ttcgttgaag ggttaacaga gtcatgagtc
     7741 agaccctttc tgcagccaca ctgctggcgg tgccgctggc gcccctggcg ggctcgctga
     7801 tcgcgggcat cctgggcacc gccttcggcg gcaaccgcat cggccgcacg gcctcgcaca
     7861 cggtcaccat cctcggggtg ctgatcgcgt tcgtgctgtc ggcgctgacc ctgcaaagcg
     7921 tgatcgccgg tgcacgcttc gaaggcaccc tctacgagtg gatggtggtg ggcggactga
     7981 agatggaagt cggcttcctg atcgacagcc tgaccgccac catgatgtgc gtggtgacct
     8041 tcgtgtcact gatggtgcac atctacacca tcggctacat ggccgacgac gatggctaca
     8101 accgcttctt ctcgtacatc tcgctgttca cgttctcgat gttgatgctc gtgatgagca
     8161 acaacttcct gcaactgttc ttcggctggg aagccgtggg cctggtgtcc tatctgctga
     8221 tcggcttctg gttcaaccgg ccgacggcca tcttcgccaa cctgaaggct ttcctggtca
     8281 accgcgtggg cgacttcggc ttcatcctcg gcatcggtct gatcgccgcc tacgccggca
     8341 gcctgaacta cgccgaggtg ttcggcaagg cgggtcagct tgcgaacctg ggctttccag
     8401 ggacggactg gctgctggtc accgtgatct gcatctgcct gttcataggc gccatgggca
     8461 aatccgccca gttcccgctg cacgtgtggc tgcccgactc gatggaaggt ccgacaccca
     8521 tttccgccct gatccacgcg gcgaccatgg tgaccgcggg catcttcatg gtggcgcgca
     8581 tgtcgccgct gttcgagctg tcggatgcgg cgctgaactt cgtgctggtg atcggtgcga
     8641 tcacggcgct gttcatgggc tttctgggca tcatccagaa cgacatcaag cgcgtggtgg
     8701 cctattccac cctgtcgcag ctggggtaca tgacggtggc gctgggtgcg tcggcctatt
     8761 cggtggctgt gttccatctg atgacgcatg ccttcttcaa ggccctgctg ttcctggcgg
     8821 cgggctcggt gatcatcggc ctgcaccaca accaggacat ccgctggatg ggtggcgtgc
     8881 gcaagtacat gccgatcacc tggatcacct tcctgctggg ctcgctggcg ctgatcggca
     8941 cgccgttctt ctccggcttc tactcgaagg acgccatcat cgaggcggtg catgccagca
     9001 agctgcccgg cgccgggttc gcgtacttcg cggtgctggc cggggtcttc gtcaccgcct
     9061 tctattcgtt ccgtctgtac ttcctggtct ttcacggtca ggagcgctac gaccagaacc
     9121 ccgacgccca ccaccatgac gatcaccacg gccatggcca cgatgcgaaa ccgcacgaga
     9181 cgccctgggt ggtctggttg ccgctggtgc tgctggccat tccctccgtg gtgatcggcg
     9241 ccatcacgct gatgccgatg ctgttcggcg atttcttccg gctcaacgac gtgatccatg
     9301 tggatgcggc gcgccatcct gccatggcgg tgctggccga gaagatccac ggctgggtcc
     9361 cgatggcgct gcacggcttc gtggcaccgc cgttctggct ggcgctggct ggcgtggcat
     9421 gcgcctacta catgtacatg gtgaacccgg ccctgccggc cgccattcag cgcgccttcc
     9481 ggcccgtcta cagcctgctg gagaacaagt attacctgga ctggttcaac gaaaacgtgg
     9541 tggcgcgggg cgcgcgtctg ctcggcaccg gtctgtggaa ggcgggcgat cgtgcgttga
     9601 tcgacggcct gctggtcaat ggctcctggc gcctggtggg ttgggtgtcc ggcatggtgc
     9661 gctggatcca gtcgggctac ctctatcact acgccctggt gatgatcctg ggcatcttcg
     9721 tgctgatgac gtacttcgtc tggctcaaca agtaaggaag acgggataag aacatgggtt
     9781 ggttgagcct tgccatctgg acgccgatcg tcttcggcgc cgtgttgctg gcgctgggac
     9841 gggacagcca ggcacagctg gtgcgctgga ttgcactcat cggctccgtg gtcagcctgc
     9901 tgatcacgct gccgctgtac accggctttc gcctgggcac cgccgagatg cagttcgtcg
     9961 agaagatgcc ctggatctcg cgcttcaatg tcaattacta ccttggcgtg gacggcatct
    10021 cgctgtggtt cgtgctgctg acggcgttca tcaccgtcat cgtcgtgatc gccggctggc
    10081 aggtgatcca gcgcaaggtg aatcagtaca tggccgcgtt cctgatcctc tcgggtctga
    10141 tggtcggcgt gtttgcggcg ctggacgggg tgctgttcta cgtcttcttc gaggccacgc
    10201 tgattccgat gtacctgatc atcggcatct ggggcgggcc acgcaagatc tacgccgcgt
    10261 tcaagttctt cctctacacg ctgctgggct cgatgctgat gctggtggcg ttcatctacc
    10321 tgtatgcggt ctcgggtggc agcttcgacg tgctcacctg gcacaagctg ccgttgtcgg
    10381 caacggcgca gacgctgctg ttctttgcca tgttcgcggc gtttgccgtg aaggtgccca
    10441 tgtggccggt gcacacctgg ctgcccgacg cgcacgtgga agcgcccacc ggcggttcgg
    10501 tggtgctggc cgccatcatg ctgaagctgg gtgcctacgg ttttctgcgc ttttccatgc
    10561 cgatcctgcc cgacgcctcg cgcgagtggg cgtggctgat gatcgcgctg tcgctcatcg
    10621 cggtgatcta catcgggctg gtggcgttgg tgcagcagga catgaagaag ctggtggcct
    10681 attcgtcggt cgctcacatg ggcttcgtga ccatgggttt cttcatcttc aacgacctgg
    10741 gcgtcaccgg tggcctggtg cagatgatcg cgcacggctt cgtgtccggt gccatgttcc
    10801 tgtgcatcgg cgtgctgtac gaccgggtcc attcgcgcga gatctccagc tacggcgggg
    10861 tggtcaattc gatgccacgc ttcggcgcct tcgcgctgct gttcttcatg gccaactgcg
    10921 gtctgccggg caccgccggc ttcatcggtg agtggatggt gatcctggcc acggtgcgcg
    10981 ccaacttctg gctcggtttg ctgctggcca cggcgctgat caccggtgcc gcctacagcc
    11041 tgtggatgtt caagcgggtc tacttcggtg cggtcgccaa cgacgacgtc cgtgccatgc
    11101 ccgacatcga tgggcgcgag ttcctggtgc tggcgctgct ggccatcgcc gtgctgtgga
    11161 tgggcctgta tccgaaaccc ttcaccgacg tcatggacgc ttcggtggtc aacctgctcc
    11221 agcacgtggc gaattccaag ctgaactgag gacgaggaca tgattgacag attcagttgg
    11281 atggccgtct atcccgagat cgtgctgctg gtcatggcat gcgtgatcac gctggtggat
    11341 ctgctggacc gcaacccgcg ccggctggcg gcctatgtgc tgtccatcct gacgctgctg
    11401 gtgctggcgg tgctgaccgg cacctacgcg cggggcggcc agacctggta cgggttcgag
    11461 ggcatggtgg tcagcgaccc gatgaccaac tggctcaagt gcttcgcggc gctggcggtg
    11521 gccgtctcgc tggtctacgc gcgcacctac gtcaccggtc gtggcatgtt gcgcggcgcc
    11581 gagtggtacg tgctcagtct gttgtcgttg ctcggcatgt acgtgctgat ctcggccaac
    11641 aacttcctgc tggtctatct gggcctggag tgcatgacgc tgtccggcta cgccctggtg
    11701 gcgctgcggc gcgaccacgt gacctcgatc gaggccgcca tgaagtactt cgtgctgggc
    11761 gccatggctt ccggtttcct tctgtatggg ctgtccatgc tctacggcgc caccggcacg
    11821 ctggacatcg gcggtgtctt caaggccgtg gcctcgggtc agatcaagca ccaggtgctg
    11881 gtgttcggcg tggtgttcgt ggtggccggg ctggcgttca agttcggtgc cgtgccgttt
    11941 cacatgtggc tgccggacgt ctacgaaggc gcgcccaccg cggtcacgct gatgatcggc
    12001 ggcgcgccca agctggccgc gttcgccatg gccgtgcgcc tgctggtgga gggcctgctg
    12061 ccgctggcca tcgactggca gcagatgctg gcggtgctgg cggtggcttc gctgctgatc
    12121 ggcaacctgg cggccatcgc gcagaccaac ctcaagcgca tgctggcgta ttccaccatc
    12181 gcgcagatgg gcttcgtgct gctgggcctg atggcgggcg tggtcaacca cagtgcgctg
    12241 gcggccgcca acgcctacag ctcggccatg ttctacgtcg tggcctatgt gctgaccacg
    12301 ctggtgagct tcggcatcat cctgctgctg tcgcgcgagg gcttcgaaag cgaggagatc
    12361 gtcgatttcg ccggcctgaa ccaacgcagc ccgctgtacg ccggcctgat ggccatctgc
    12421 ctgttctcgc tggccggcat tccgccgatg gtcggcttcc aggccaagct ggcggtgctg
    12481 caggccctgg tcagcaccgg cctgccgtcc tacattggcc tggcggtgtt cgccgtcatg
    12541 atgtcgctgg tgggtgcgtt ctactacctg cgcgtggtca aggtgatgta cttcgacgag
    12601 ccgctgtcga gcgcgccgat ccaggcgccg ttcgacatgc gcgcggtgct gtccgtcaac
    12661 ggcctgctgc tgctggtgct gggcatcgtg ccgggctcgc tgatggcgct gtgcgccaag
    12721 gccgtggtcc agatgcttgc gacttgagga tgtcgcagaa cctctccatc tggctgctga
    12781 ttttgctggc cctggtggcc gccaatctgc cgtttgtcaa ccatcgtctg atggcggtgt
    12841 ttcccctgcg cacggcggcc aagggtctgg cgtggcgcct gctggagctg ctgctctggt
    12901 atggcttcgt gatcggcgtc ggcatggcga tcgagcgcgc ccagggtcag aaccagcccc
    12961 agggctggga gttctatgcc gtcacggcgg cgctgttcct gaccctggcg tttccgggct
    13021 tcgtctatcg ctacctggtg cgtcgggggc gttgatggcg tccgatttgc gcgagacccg
    13081 cgtcggtggg caggagctgt tcaagggcga cttcctgcac gccatgcgtg accgggtgcg
    13141 gctgcccgat ggtcagctca cacagcgcga atacatcgtg cacccaggcg cggtgatgat
    13201 cgtgccgtgg ctgcaggggc cggatgggtc gctgcaactg gtgctggaac gacagtatcg
    13261 ctatccggtg cagcagacca tcgtcgagtt tccggccggc aagctcgatc cgggcgaagc
    13321 gcctctggtc tgcgctcagc gcgaactgcg cgaggagacc ggctacaccg cccgcgagtg
    13381 ggcgcgggcc ggcctgttgc atccggtggt ctcctactcg accgagttca tcgaggtttg
    13441 gttcgcccgt ggcctgcagg ccggcgagcg gcggctcgac gagggtgagt tcctcgaggt
    13501 cttcacggct tcccccgagc agttgtatgc ctggtgccgg gatggggaga tcaccgacgc
    13561 caagaccctg atcggggcct tgtggctgca gaatgtggcc agcggcgcct ggtcgctgaa
    13621 ctggcaaccg gccgatccgg cagcatgacc ccgcaatgaa agtcctcaac ctgcaatgtg
    13681 cccacggcca cgcgttcgag ggctggttcg cgtccgagga cgacttccag gaccagcgca
    13741 cgcggcagct tgtcgaatgc ccgctgtgcg gtgacgtcga ggtgaccaag ctgcccagcg
    13801 ccccgcggct cagcctgagc ggcgccagtg cggccgacac cgcatcgtct gcgtcggcaa
    13861 ccgggccgac cgctgcagcg gcctcggtgg ccaacctgcc accggcggcg ctgcaggccg
    13921 cctggatgca cctggtgcgc caggtcatgg cacgcaccga agacgtgggc gagcgctttg
    13981 ccgaagaagc gcgccgcatg cactacggcg aaaccgaaga ccgtggcata cgcgggcagg
    14041 ccacgccggc cgagaccgaa gcgctgctgg aggagggcat cgccgtcatg ccgctgcccc
    14101 tgccaccggg cttcaagggt acgttgcaat agtcctagat gacttcgata gctgccggcg
    14161 ctttcctgaa ggggccggag cttgattggg caccgagggc cgcgcctggc cgccagcctc
    14221 gaccaaagcc ggctgtgggg ccccgtctca gctgcaactg ggggcgttga cgatggcgcg
    14281 cagcgcttcg gtgttcagga tgcgcacgtg gcgctgcttg acttcgacga tgccttcctc
    14341 ggcgaacttc gagaaggtgc ggctcacggt ttccagcttc agccccaggt agctgccaat
    14401 ttcctcgcgc gtcatgcgca gcaccagctc cgactgcgag aagccgcgcg catgcaggcg
    14461 ttgcaccagg ttcagcagga aggccgccag gcgctcttcg gcgcgcatgc tgcccagcag
    14521 cagcatcacg ccatgctcgc gcacgatctc gcggctcatg atcttgtgca cgtgcgactg
    14581 cagcgcctgc acctctcgcg acagctcttc gatgcggtcg tagggcatga cgcagacttc
    14641 ggcgtcttcc agcgccaccg catcgcaggt gtggcggtcg cccacgatgc cgtccaggcc
    14701 gatcacctcg ccggccatct gaaagcccgt cacctgatcg cgcccgtccg cggtggcgac
    14761 gcaggtcttg aagaagccgg tacggatggc gtacagcgcg gtgaagcggt cgccgttggc
    14821 aaacagcgag gtgccgcgct tgaccttgcg ccgagttgcc accaggtcgt cgagcttgcc
    14881 catttccgtg tccgacaggc ccatcggcat gcacagctcg cgcaggttgc agttggagca
    14941 ggcgactttg atggcttgtg gagtcatgcg ggcgtcgggt ggtcagtgcc gggcacggta
    15001 catgcgcggt gacgctgcat tctaggcgat cggggctggt gccggtggat tcttgatgcg
    15061 gctcaaaatc catccgtgcc ggaacgggta cttgatcgcc gtcaaggcgc gatcgcgtca
    15121 gcccggcgac agttgagcct gacgtcaaag gacttattgg atgcaagcca ttacccccga
    15181 acttctcacc aagttcgacg tgccggggcc gcgttacacc tcctacccga ccgcagatcg
    15241 ctttgtcgag gccttcggcg agcccgacta cctgcgggcg ctggatcagc ggcgcaccgg
    15301 gccggtggcc ggtgcgcggc cgctgtcgct gtacgtgcac atcccgttct gcgaatcgct
    15361 gtgctactac tgcgcctgca acaagatcat caccaagcac cacgaccgca gcgcgcccta
    15421 cctgcgctac ctgagcaagg aggtggacct gcacgtgcag cacctcggcg ccggccagtc
    15481 ggtcagccag ctgcatctgg gcgggggcac gcccaccttc ttcagcgacg acgagctggg
    15541 cgagctgatg agcatgctgc gccgcaactt caacctggtg gcgggcggtg aatattccat
    15601 cgaagtcgat ccgcgcaccg tggacgcaac gcgcctgcag accttggcgc ggctgggctt
    15661 caaccgcctg tcgttcggcg tgcaggactt cgatccgctg gtgcagaagg cggtgcaccg
    15721 cattcagccg gccgaacagg tgttcgccct ggtggacgcc gcgcggcgca tcggcttcga
    15781 gtcgatcaac gtcgatctga tctacggcct gcccaggcag acgcctgaat ccttcgatcg
    15841 cacgctggcc caggtggccc agttgcgacc cgaccgcatt gcgctgtacg gctacgcgca
    15901 cctgcccgag cgcttcaagc cgcagcggcg catcgtggtg gtcgatctgc cgggcgccgc
    15961 caacaaggtg gccatgctgg cgcgcgcgct ggcggcgttc gacgccgccg gctacgacta
    16021 catcggcatg gaccacttcg cgctgcccga cgacgcgctg gccgtggcca agcgccaggg
    16081 gcggttgcac cgcaacttcc agggctacag cacgcagccc gactgcgacc tgatcggtct
    16141 gggcgtgtcg gcgatcggca agatcggtgc cacctacagc cagaacgcca aggcgctgga
    16201 agactaccag gacctgctgg accagggcca cctgccggtg gtgcgcggcc tggcggtgtc
    16261 gcgcgacgac ctgctgcgcc gcgccgtcat catggccctg atgtgccagg gcgaggtgct
    16321 gtacgaatcg atcgagctgg cgcacctgat ccgctttcag gactacttcg ccagcgaact
    16381 ggaaaccctg cgcggcatgc aggcgcaggg cctggtcagg ctcaccgaca ccggcatcga
    16441 agtcacgccc atgggctggt tcttcgtgcg cggcgtggcc atggtgttcg acaagtacct
    16501 gcaggccgac cgcacccggg cgcggttttc taaaatcatc tgatgcaatc ttccctggcc
    16561 gtgaccgccc tggtcatggg gctggccggc ggtccgcact gcatcgccat gtgcggtgcg
    16621 gcctgcgccg gcatcgggca ggcggcggcg ccgcgaagca cccaggccct ggcgctgttc
    16681 cagctcggcc ggctgaccgg ctatgcggcg ctgggcgcgc tggccgccgc caccatgcag
    16741 ggcctgggct ggctcacggt gcagtcggcc gccttgcgcc cggtgtggac catggtgcac
    16801 gtggctgcgg tgctgctggg tctgtcgctg ctggcgttcg cgcgccagcc ggtctggctt
    16861 gaagccggcg cgcggcgcgt ctgggcacgc gtgcgcaccg ccacgcagtc gctcggcctg
    16921 gtggctccgc tgggcctggg tgtggcctgg gcgctgctgc cctgcgggct gctgtactcg
    16981 gcgctgatgg tggcggccct ggccggcagc gtggtcggtg gcgcggccgt catggccttg
    17041 ttcgccctcg gctccggcct gtggctgggt ctggggccgt ggctgctgct gcggctgggc
    17101 cggtccgggc gcggcgcctg gagcatgcgc gtggccgggt tggcgctggc cgccacctcg
    17161 gcctgggggc tgtggatggg gctggtgcac gaccaggcgc cctggtgcgt ggtgcccacg
    17221 ccctgacagt tgctattatt tgggtagctg ctggcgcttg ctgcacaagc gctggggcca
    17281 gaaaagcctg tgattctggc cgggcggccc cggtcagccc gtcagcgcgc cgatgccctt
    17341 gttcagcatg tacaaggcca gcacgaacaa cacgccggca aaggcgcgct tgagcttctt
    17401 caccggcagc gcgtgcgcgg ccctggcgcc caccggcgcc atcagcacgc tgcacgaagc
    17461 gatcaccacc agcgccggca gccacaggta gccgaacgaa taggccggca gcccctgcag
    17521 gccctggccg gcgacgatgt agcccagcac gttcgacagc gcaatcggaa agcccagcgc
    17581 cgcgctggtg gccaccgcgt tgtggatggc cacgttgcac caggtcatga agggcacgct
    17641 gacgaagccg ccgccggcgc ccaccaggcc cgacagaaag ccgatcacgc tgccggccgc
    17701 cagctggccg gtggtgcccg gcaactggcg gctggcggcc ggcttcttgt ccaggtacat
    17761 ctgcacggcc gagaaggcca cgaagccggc gaacacgaag tacagcgccg cccccttgag
    17821 cagcgcaaac acgcccaggc ttgccaccag gccgcccagc acgatgccgg gggccaggcg
    17881 cttgacgatg tcccagcgca ccgccccctt cttgtggtgc gcgcgcacgc tgctcatgga
    17941 ggtgaagatg atggtggcca tcgaggtggc gatggccatc ttcaccgcca ggccgggcgc
    18001 cacctcgcgc cccaccagca tggtcgagat gaagggcacc atgatcatgc cgccgccgat
    18061 gcccagcagg ccggccagaa agccggtggc caggcccagc gcggccagtt cgatgaaaag
    18121 tagcggttcg atcggcatcg gggtggtggc atcgagcttg cctggcgcgg cacgggcgtc
    18181 aagcaaaaag gccggacacg cgccggcctg ggaaatgagg tgtctgcgaa tgccaccgga
    18241 ccgtcatcct gaacgcgggg gttcaggtgg gcgaggtcag actgatcttg ggttcatctg
    18301 acgcgagacg atcacgccga ccagacgatg ttccaagccc gggctcatac gagcattggt
    18361 tcaaggaaat ataaagcccg gcatgcaccg cagacggctc gattctatcg cctcgggcgt
    18421 ccgtcgctgc gcgtgccgcc cggcggggtg gggcggccgg cgcacgcggc tacagcaact
    18481 ggccgtcgtt gcccagcgcc tggtccagcc gggccagcag ggcgcccagc gcggcctggc
    18541 tggcgggctc gtcggcgccc gccggggtgt cggtgggcgc cgtggtggcg gcaccggcgg
    18601 ggcgcggcgc gtcggtcagt tcaaaggcca ggttcagcgc cgccagcacg gcgatgcggt
    18661 cgcgcgcctt gatgcggccg gcatcgcgga tcttgcacat ggcggcatcg acccgttgca
    18721 ccgcctgctg caggcgctgc tcgccgcctt ccggcgccgc cagcaggtag gcctggccca
    18781 tgatgcgcac ttcgacctgc ttcatcgcgt ggcctccttg cctgcgggtg gcgtatcggg
    18841 cgtggcttcg ccgctcagca cgccggcggt ggccggcagg cgatcgatca gcgaatcgat
    18901 gcgcgcgcgc gccgccatca ggcgcgatcg caggttgtcg cgctcgtgcg tcagcgcctg
    18961 cacctgctgg gccagcaggt cgttggtgcg ctgcagttcc tcgtggcgca cgagcaggcg
    19021 ctcgacgcgc tcggacagct gttcgatcag gggcggggtc gtcatcggga gggcaccgtg
    19081 tcgctgaagg cgccccgatt gtaggcggcg cgcgacaggc gcccgctaca atcgccaccg
    19141 ttggtgctcg cgcgccgtga ctctcacggc cgcagttcaa cgggaagcag gagggcatgc
    19201 cgaccgaccc ggtcgccgcc taacctgcgc tgcccccgca acggtaagcg gatggcgtcg
    19261 tgacccgtca cggcgccgac tcctgcctca caaggtcact ggcgccgcgt cctcgacgcg
    19321 ctgccgggaa ggccaggcga tcacggagcc aattccgcca gcccggatac cggccaacga
    19381 ggcgacgtcg cccgcgcagt gcccgccagg cctgccgtgc ctcgtcatgt cgccccattt
    19441 gccggcgggg aagccggccg gggctgctgc atcccgtgtt tcccgtcatg tcccttcgtc
    19501 tgcaatcctt ccggccggct gcgctgccgc tggcgctcgc ctgtgccttc tgcgcccatg
    19561 cggcgcagga caccaccgtc gcccagctgg ccgcgcccag cctgcccgac accgtggtga
    19621 ccgccacccg cgtggcccaa cccctgaccg acgtgctggc cgacgtcacc gtgctcggcg
    19681 acgagcagat ccgcgccagc ggcgcggtca gcatcggcga tctgctggcg cgccagccgg
    19741 ggctggagct ggcccgcaac ggcggcctcg gcagcgccac cagcgtgtac ctgcgcgggg
    19801 ctgaaacccg cttcaccgcc gtctacatcg acggcatccg catcgactcg caggccaccg
    19861 gcggcgcccc ctgggaggcc atcgcgctgg gccaggtcga acgcatcgaa atcgtgcgcg
    19921 gcccggccgc cgccgtgtac ggctcggacg ccatcgcggg cgtggtgcag atcttcaccc
    19981 gcaagggcga aggcccgttc gcaccctatg tcggcgtggg cctgggcagc caccgcagcg
    20041 gcaagctgga ggccggcttt tcgggccgca acggcgcgct cgactacgcc ctgggcgccg
    20101 cgcgcgagct gagccgcggc tacaacagca tgccggcggg caaccccgac cgcgacggct
    20161 accgccagag ctcggccagc ggccgcctgg gctggaacat caacgacgcc caccggctcg
    20221 aactgagcgg cacctacacc gacatcgacg cccagtacga cggctttctg cccggcctgg
    20281 acgaccacgg cctgaaccgc ctgggcaccc tgggcgccgc ctggtcggcg cagtggagca
    20341 agcgctacag cacccgcctg tcggtgagtg aatccgacca gcgctacgaa accgtgcctt
    20401 cgccctatct caccaagacg cgcctgcgca actacctgct tcagaacgaa tggcgcgaag
    20461 gcggccacct gatcaccgcc acgctggagc ggcgcgaaga ccatctggtc aacgccccga
    20521 tcgcgcaggg caggcaccag aatgccatcg ccctgggcta cggcttcagc ggcgggccgc
    20581 acaccctgca ggtcaacctg cgccacgacc gcgacagcga gttcggcggc cagaccaccg
    20641 gtggcgtggc ctacggctac ggctttgcgc ccggctggcg cgccaccgtg gcggccggca
    20701 cggcgtttcg cgtgcccacg ctgtaccagc gcttttccga atacggccag cctgcgctgc
    20761 agccggaaaa gggccgcaac gtcgagctgg ggctgcactg ggcgcagggc gacagccgct
    20821 tcggcgtcag cgtctggcgc aaccgcgtca gcaacctgat cgtgttcggc gcccccggcc
    20881 cctgcgccgg cgctttcggc tgctacgaaa acgccggccg cgcgcgcctg cagggcgtga
    20941 cgatcagcgg cgcacaccag ctgggcggcg tcaacctgag cgccgcgctt gacctgcagc
    21001 aaccccgcaa cacgctcacc gacaagctgc tgccgcggcg tgcgcagcgc ctgctcaagc
    21061 tcagcgccga tatccgtgtg ggcggctgga cgctgggcgc cgaatggcag gccagcagcc
    21121 accgctggga caacgccgcc aacacccgtc gcctggccgg ctacggcatc gtcaacctgt
    21181 acgccagcac caccctggcg cgcgactggc aggtgctggc gcgcctggac aacctgggcg
    21241 acagggacta ccagctggcg cacggctacg ccacgcccgg ccgcagcctg ttcgtcggcc
    21301 tgcgctggac gccgcgctga gcgcggcaag cggggcagcg ggcatggacg tcgcgcgcag
    21361 cgagctgatc ctgggcggcc agaagagcgg caagtcgcgc cgcgccgaaa gcctggccgc
    21421 cgcctggctg gcggccgcgc ccacgcaccg ggcggtgctg atcgccaccg ccggcgcgca
    21481 cgacgccgag atgcgcgccc gcattgcccg ccaccagcgt gagcgcgccg cgcgcgtgcc
    21541 cgggctggtc acgcacgagg cgccgcacga actggccgcc gccatcgccc aggccggcgg
    21601 cgccgaaacg ctgcgcatcg tcgactgcct gaccctgtgg ctcacgcagc gtctgatgcc
    21661 gctgcccggt catgaatccc atgggcctgc ggcgcttgat gggcaagcgc cggcagctat
    21721 cattattgaa gcactgcgaa gctgccccgg ccccatcgtg ctggtcggca acgaaatcgg
    21781 cctgggcgtg atcccgctcg gcgccgaggt gcgcgccttc gtcgacgcgc tgggccagct
    21841 caaccaggcg gtggccgcgc gctgccagcg cgtcacgctg atggccgccg ggttgccgct
    21901 caccctgaag gacgccgccc catgagccgg tggctgctgg ccctgctggc ctgggtctgg
    21961 gtcggcctgg tgtcggccca ggagctgcgc gacgacgccg gccagccgct gcgcctggac
    22021 gccgcgccca ggcgcatcgt cagcctgctg ccttcgctga ccgaaaccgt gtgcgcgctg
    22081 ggcgcctgcg agcgcctggt cggcgtcgac cgctactcca actggccagc gagcgtgcag
    22141 accctgcccc aggtcggggg cgggctggac ccgaacatcg aggcggtggt ggcgctcaaa
    22201 cccgacctgg tgctgatcgg cacctcctcg cgcgcggcgc tgcggctgcg cgccctgggc
    22261 ctgaacgtgc tggcgctgga gccgcgcacg cgcgccgagc tgcgccgcgt cacgcagcgg
    22321 ctgggtaccg cgctgcgcct ggacggcgcc gacgccctgg cgcggcgcat cgacgacggc
    22381 gtggcggccg ctgcggcggc gctgccggtg gcggcgcgcg gccagcgcgt ctactacgaa
    22441 atcaccccgg cgccgcacgc cgccgggcag ggcagcttca tccacgagct gctgctggcg
    22501 ctgggcctgg tcaacgtcat cgacgatccg gcgctggggc cgttcccgcg cctcaacccc
    22561 gagctggtgg tgcgcgccga ccccgacctc atcatggtca gccagggcgg ggcggccgac
    22621 atggcgcgcc gccccggctg ggcaggcctg cgcgcgctgc gggaaggccg ggtgtgcgaa
    22681 ttcaacgccg cccagcgcga catcctggtg cgccccggcc cgcgcatgcc cgaggcggca
    22741 cgcctgatgg cggactgcgc ggcgcgccgc ccacttcaaa accgatagct ggccgcgccg
    22801 aacggaactt ggctgacggc ccgatgaacc tgcacactcg cgccatggac gcgcccgccc
    22861 acgccgccac cgccgctctg ccaccgcagg gcggggcgcg cgtgcgctgg ctggcgctgg
    22921 ccctgctggc gctggcgctg ctcggtctgg ggctgggggc cagtgtcggc agcaccggtt
    22981 tcgacagcct gctgcgcgcc tggcacgacg ccgtcgcctg gcagatcgtc tgggacatcc
    23041 gcctgccgcg caccctgggc gcctggctgg ccggcgcgct gctggggctg gccggcgcgg
    23101 tggcgcaggg gctgtttcgc aacccgctgg ccgacccgtt cctgcttggc agcgcctcgg
    23161 gcgcggcgct ggcggtggcg ctgacctttg ccgcgctggg cgccggcggc ctggtggcgg
    23221 gcggccacgg cgtggcgctg ctgcaggcgg cctcgccctg gctggcgcgc ctgggcttca
    23281 ccggcgccgc cttcgtcggc gcgctcggcg gcgtgctgct ggcgctggtg ctggcgcagg
    23341 gcgtgcagca gacgctgcgt ctgctgctgg ctggcgtggt ggtgggctac gtgctgggcg
    23401 ccgtgcgcga gctggtgcaa ctggcccagc ccgacgtgct gcaggccatg cagggcttca
    23461 gcctgggtgt caccggcttc gtcggctggg gcgcctgcgc cgtcatggcc gccgtgctgg
    23521 cgccgctgct gccgctggcc tgggcgctgg cgcgcgggct cgacgcgctc agcctgggcg
    23581 aagccacggc gcgcagcctg ggcctgccgc tgggcctgct gcgcgcgctg ctgatcggcg
    23641 tgctggcgct ggccaccggc accgcggtgg cgcagaccgg cctgatcggc ttcgtcggcc
    23701 tggtggcgcc gcacctggcg cgcgcgctgg cgcgctgcac gcacgggccg ttgctgctgc
    23761 tggccagcct gatgggcggc ctgctgctga ccgcggccga catcgcggcg cgcctgctga
    23821 tcgcgccgca ggagctgccg gtgggcgtgc tcacggccgt gctgggcggg ctttacctgc
    23881 tgtggctgat gcaccgccgc gggcgcgccg ggggggcgtt gcagtgaccc aacttgctac
    23941 gcaaaccata gctgtcggcg cacgtctggc aagcgccagc atcggaaaac accagatact
    24001 gcacgacatg gcgctggagc tgccgcaggg ccgctggtgc gccgtcgtcg ggcccaacgg
    24061 cgccggcaaa tcgaccctgc tgcgcgtgct ggcggggctg ctgccgtgcc agggccagat
    24121 cagcctgtgg ggccgcccgc tgcacgactg gccggcgcgc gcgcgcgccc gccagctggc
    24181 ctggctgggc cagcaggaag gcggcggcga agacctgctg gcctgggaca tcgccatgct
    24241 cgggcggctg ccgcatcgcc cctggctggc cgatgccagc gcgcacgacc aggccatcgt
    24301 cgccgacgcc ctgcgctcca tgcaggtctg ggcgctgcgc gggcggcggc tgggcgagct
    24361 gtcgggtggc gagcgccagc gcgtgctgct ggcgcgcctg ctggcggtgc aggcgccgct
    24421 gctgctgatg gacgagccgc tggcgcacct ggacccgccg caccaggccg actggctggc
    24481 cgtggtgcgc gcccaggtgg ccgccggcgg caccgtggtc agcgtgctgc acgagctgaa
    24541 catggcgctg gcggccgacg acatcgtggt gctgcgcgcc ggccgcctgc tgcaccacgg
    24601 ccccgccgcc tgcgcccaca cccacgccgc gctgcgggcg gcgttcgacg gccgcatcag
    24661 cattcgccag ctcgaacgcc aatgggtggc gctgccccaa cccgatcgaa gcgagcctga
    24721 ccatgcagat tgaatctccc cccaccgaac aacgctacga gcgccccgca ggtgaacgcc
    24781 gcggtctggt catcgtgcac accggcgacg gcaagggcaa aagcacggcc gccttcggcc
    24841 tggcgctgcg cgcgttcggc cgccagcatg tgcacggcaa gcaggtggtc atctatcagt
    24901 tcatgaaggt gccgaccgcg cgctttggcg aacaccgggc gttcgagcaa ctcggcctgc
    24961 cggtgcacgg cctgggcgac ggcttttcat ggaagagcca ggacctggag cactcggccc
    25021 agctggcgcg cgaaggctgg cagaaggcgc gggcagcgat cctggacggc cagcactttc
    25081 tggtggtgct ggacgaaatc acctacccgc tgatctacgg ctggctgccg ctgggcgacg
    25141 tgctgcacac cctgcgccag cgcccgcgcg aggtgcacgt gtgcctcacg ggccggcgct
    25201 gcccgcccga gctgatcgag ctggccgaca cggtgacgga gatga
//
LOCUS       DAOOOG010000017        24633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_1912_length_24633_cov_25.457197, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000017 DAOOOG010000000
VERSION     DAOOOG010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 24633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24633
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1912_length_24633_cov_25.457197"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PK242_02750"
     CDS             complement(<1..694)
                     /locus_tag="PK242_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOZ92970.1"
                     /translation="MAQITIEHHGKVTLFTINRPARKNAICKDTALELQAGFAEFDAS
                     EQRVAVITGAGNDAFSSGADVSNLPELWRCIPGSGFTTDKPIIAATAGWVVGGAMVLT
                     MMCDLIVAAENTRFSYPEARLGFTGGMIAGLAARIPHKAAMDLMLLGRTMDAQRAYGI
                     GLVSDVVPTGKQLEIAMEQAQTLAASAPLVLTTIKRFVNQHVLPKSPAEAMSETLRQL
                     NVVRESADIKEGM"
     gene            complement(734..2860)
                     /locus_tag="PK242_02755"
     CDS             complement(734..2860)
                     /locus_tag="PK242_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOZ92971.1"
                     /translation="MTSPTSSHSLKSLLNPRSVAVIGASEDQTKFGGRLYKTLLQHHY
                     DGAVYPINPTRDQLFGLKTYPSVTDTPQAPDMVVMALPRDKVKDEIAACAARGAKAGI
                     IITSKFSDAGPEGLALEREVVATAAAHGMRLIGPNCLGLISPANKLVLCSSPAVNVPQ
                     LIEAPIGFISQSGALMGTLFDRSYGMGIGFSHCVSVGNQADLELNDFVEFLIEDERTQ
                     VICSYVEGIKTPQRFVALARRARAAGKPWLMVKAGATDDGRRAAYSHTASLAGDFAAL
                     KAICERENVVLMDDPLDMLSLARAMVRYPRRQVRNVAVFTTSGGGGAITADQLSRAGV
                     GLSTFSDATMAALSEHYSEGQGANPIDVGGRKPDATSDIGAVTAELALADPGVDLGLM
                     VLTTAPDVPGLTRQMADGADRPAAGGKPTLYVMLPGRVAAPAREFLVQRSLPYVDTLA
                     EAMAVLRGWKAWSTYQEPAAPVRPADMAATPVPAAGTLGEAASKTLLGQAGIPVNIER
                     LVRSADEAAEAAAELGFPLVLKVVSPDIAHKSDVGGVALNILDPDDLRRRLALMQASV
                     ARAAPSARIDGFSLQRQEAGELELIVGARLDPQFGPQVLVGAGGVLVELLKDIAVLPA
                     PVDAASARRAVENLKIAPLLRAYRGRAALDVDAAVDVIVRLGWLAHDLAGSSGRDFEI
                     EVNPLKLRQQGHGAVAVDARARIAQA"
     gene            complement(2894..3652)
                     /locus_tag="PK242_02760"
     CDS             complement(2894..3652)
                     /locus_tag="PK242_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOZ92972.1"
                     /translation="MSQPELLSRRDGPVLHLQLHRPEVGNALSAALVQALTEAVDAAT
                     HDVSVRLLVLSGAGRHFCTGFDLSDLDRETDDSLLARFTRVELLLQAVHAAPFATLAL
                     AHGRTMGAGADLFAACGDRWIVGDAGFAFPGAAFGLVLGTVRLTELVGAPHAAAWIEG
                     GQRIDADQALACGLATRHIETEQIDAELGRWGARVNRLDAPTQRAIQAAIAAGRRPRG
                     DAGDAQDLARLVRSAARPGLRDRIAAYRAAQRKG"
     gene            complement(3649..4242)
                     /locus_tag="PK242_02765"
     CDS             complement(3649..4242)
                     /locus_tag="PK242_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HOZ92973.1"
                     /translation="MAAESPLLTFVAAMTRLVESGPADEARLLDGARPLMKALVAQDD
                     WLPDAMAAAHPQYYQQHLLYGDPLDRFSLVSFVWGPGQKTPVHDHTVWGIIGMLRGAE
                     CAQRYTRDHQGRVTACGAEETLRPGDIDVVSPTLGDIHVVRNGLEDQVSISIHLYGGN
                     IGRIARHVFVPETDTVKDFVSGYSNTLSPNLWARQAA"
     gene            complement(4242..5441)
                     /locus_tag="PK242_02770"
     CDS             complement(4242..5441)
                     /locus_tag="PK242_02770"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOZ92974.1"
                     /translation="MTRSAFDSAAASGSAAPLLPLAGVRVVEFCTTASGPFSTMLLSD
                     MGADVVKIEPPTGDGLRQWPPLNQGYSENFASLNRNKRSAVLNLKDPQDVALARRLIL
                     EADVLVENNRPGVMDRLGLGYAALSAEKPELLFCSISAYGQSGPRASEGGFDLTIQAA
                     AGVMSVTGEPAGAPVKCGVPLSDFASGLYAAFSIASMLAHVRAGGRGAHIDIPMFGCT
                     LGIAALQTSEYFGNGRSPGKLGSAHPRNAPYQAFQAQDGYFAIAAGNHKLWLDVLRVV
                     QMPALAEDPRFLSTTDRARNQQALKEILEEVFTQRPVAHWIDAFNQAGVPHARINDYA
                     AALADPQTVHMGWVQPLTLPNGQQTRTFASPVRLNGQGFPIRRSPPELGQHTQEIRAE
                     CARSEVN"
     gene            complement(5542..6291)
                     /locus_tag="PK242_02775"
     CDS             complement(5542..6291)
                     /locus_tag="PK242_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ92975.1"
                     /translation="MAAVNPPALRIDQLIVQRGSAQVVNGVSFSVPHGGSLGIVGESG
                     CGKSTILRAVAGIDTQWTGRIEIAGRPIGVQRSLADRRAMQMVFQDPLAALNPSHTID
                     EILREPLIVHRMPDQDARIVQALQTVALPLAVRFRFPGQLSGGQRQRVCIARALLVEP
                     QVLLLDEPTSALDVSVQAEVLNLLSRLRRERQQAFVLVSHDLHVVAHMCDQIAFMVGG
                     QFTDVLSRDRLRGATLHHPEAQRLLDAALTH"
     gene            complement(6279..7136)
                     /locus_tag="PK242_02780"
     CDS             complement(6279..7136)
                     /locus_tag="PK242_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ92976.1"
                     /translation="MNHIPRDSAPPLLDIDNLRISFQTAHGRTEAVRGISLRMGRERV
                     AVVGESGSGKSITFRSLFGLLPATASVRADRMQLQGQDLLSLDRRDLRAIRGRRIGMV
                     VQDPKQGLDPLMTIGRQLIEMLRLHRKVPAAGAYQRAVELLAEVHIRDPKRVMGLYPH
                     EVSGGMAQRAMLAMILSAEPELLIADEATSALDAVVRHRILDLIEEQVRLRGMGLVLI
                     SHDLDLVARYADRILVMYAGRVMEELRGGELSAAQHPYTRGLLACRPSLDHPGQDLPV
                     LQREASWLQ"
     gene            complement(7144..8049)
                     /locus_tag="PK242_02785"
     CDS             complement(7144..8049)
                     /locus_tag="PK242_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ92977.1"
                     /translation="MNPSSTLRQWLASDTPATRTQAKLGAIYRLARRFLTHPIGVLGL
                     AMVASIVLAAGLAPWLPLADPIAQSLDQRLLPPSAAHWLGTDTLGRDILARVVHGARP
                     TLAIVALVLVISVPVGLLVGAAAGLWGGWIDAVLMRVADVFLAFPRLILAIAVAAALS
                     AGLWTAVIAIAATGWPPYARVARAEIAALRRSEFVQAARAIGVSPLRLLFRHALPLSL
                     PSAFVRAALDAPGIVLIVAGLGFLGVGLPPPQPEWGAMVAEGRAIVFEAWWVSTLPGI
                     MILWLSLGFNFVGDAMRDVVDPGVK"
     gene            complement(8046..9131)
                     /locus_tag="PK242_02790"
     CDS             complement(8046..9131)
                     /locus_tag="PK242_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009914885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ92978.1"
                     /translation="MATAAADDGVDIAPRRRLRSVRLRRGLRWLGATLLTSCITLFGL
                     LVVTFALGRVLPADPVLRIVGDRASQEQYDEVFRQLRLDRPLTEQFAAYVGDMVRGDF
                     GLSLVTRNPIGSDIARYFPATFELATVAIILGVVFGIPIGVVCAQYANRLPDHLGRML
                     SLIGYSVPVFWLGIVGLLVFYARLEWVAGPGRLDTAYRYSVPEVTHLMLIDTVLAGEW
                     DAFRNALSHLVLPASLLGLIALGYIARMTRSFLLWQLRQDYTTVLRVKGLSEAAIIWR
                     HALFNAAGPVLAIVALTYAYLLEGAVLTETVFAWPGLGMYITDSLFASDVPAVLTATL
                     LVGVIFMVVNIASEVIQAALDPRSRLA"
     gene            complement(9137..10732)
                     /locus_tag="PK242_02795"
     CDS             complement(9137..10732)
                     /locus_tag="PK242_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ92979.1"
                     /translation="MKKSMRQVLSTSVLAACLSATATWAATPADTLVIARDIGTILSL
                     DPQEAFEIAPGDALNSLYLRLVQHDPADFSKIVPGVAQSWQAAPDGKQIVFQIRKDLK
                     FQSGNPVTAEDAAFSLQRGILLDKQPAIILRQFGWTKDNVKQRVRADGDKLVLSFDQP
                     YSTELILSALSAAIASVVDMKLVMSNERNGDLGNGWLRTRSAGAGAYKLVEWKAKDVV
                     VLEAFPGFKGGSPAKLKRVILRHIAEPATQRLLLEKGDVDVASDLKADQLQSIKGNAN
                     LKMIQIPRGTVYYLALNTANPNLSKPEVWQAMRWLIDYDGIANKLFSGQYRVNQSAVA
                     RGTPGALTEEPYKLDPAKAKALLAKAGFPNGFSMSLETSGASPYHEVSQSLQSTFAQG
                     GIKVELRMGEPSQVMTRYRERRHDALVFVWAPDYSDPSSTLEFFSRNTDDSDKASNKN
                     APWRAHWLTPQLTAEADKASHEVDDAKRMKIYADIQRKLRDESPFTFLIQKLEPIAMR
                     TNVHNYSGAVTFDSTPYWMIEKR"
     gene            complement(10801..12231)
                     /locus_tag="PK242_02800"
     CDS             complement(10801..12231)
                     /locus_tag="PK242_02800"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOZ92980.1"
                     /translation="MYESLALYIDGEFIQGGGRVEQPVLDPATGNTIGQLPHATRADL
                     DRALAAAERAFATWRHVSPMERSAILRKVGQLSRERAKDIGRNITLDQGKPLAEAVGE
                     VLRCADHCDWHAEECRRIYGRVIPPRTPNVRQFVLREPIGVCAAFTPWNFPYNQAIRK
                     IAAAIGAGCTIVIKGAEDAPSAVVAIARLFHEAGLPPGVLNVVWGVPHEVSEHLIASP
                     IVRKVSFTGSVPVGKQLAALAGSHMKRITMELGGHSPVIVCADADVERAATMLAASKF
                     TNAGQVCVSPTRFYIQEPVWDRFMASFTELSRQIKVGPGLEADTRMGPLAHARRVTAM
                     AEFVEDAAARGARIETGGTRLGEVGNFFAPTVIAAPPDDARIMQAEPFGPIAACVPFK
                     DLDEALQRANSLPFGLSSYAFTSNTRHALQIQNGLEAGMVNINHFGQALPETPFGGIK
                     DSGMGSEGGSETFDGYLVTKFVTQMD"
     gene            complement(12279..13664)
                     /locus_tag="PK242_02805"
     CDS             complement(12279..13664)
                     /locus_tag="PK242_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOZ92981.1"
                     /translation="MSSTPSPNWQKDIDHVVHPYSNLDAHDRRGPVVITRGDGCYVED
                     ENGKRYLEGMSGLWCASLGFSEQRLIDAAMAQFRTLPYYHLFNHRSHPPGIALAEALT
                     AIAPAGLNHVLFANSGSEANDAAVKLVWYYNNQLGRPSKKKIISRINGYHGVTVASGS
                     LTGLVHVHRDFDLPIPQVLHTDCPSHYHFALPSETEAQFAERLANNLEQLILKEGPDT
                     VAAFIAEPINGSGGVIVPPAGYFDRVQAVLKKYDILFIVDEVICGFGRTGQMFGSDSF
                     QLKPDMMTVAKGLSGAYQPISGLLITDQIHEALKAGSRKLGAFAHGLTYAAHPVAAAV
                     ALETIRLYQERRIVEHVQALSPYFFQRLQGLADHPLVGEVRGMGLLAAVEITADKASK
                     TPFTPDKAVGAWIQNKAMEHGVIVRAIANCIALCPPLISERRHIDELVDGLTRAVDAA
                     QVQFGASLATS"
     gene            complement(13828..14856)
                     /locus_tag="PK242_02810"
     CDS             complement(13828..14856)
                     /locus_tag="PK242_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOZ92982.1"
                     /translation="MTTRLLVGAISGEATRLATHVLHRLLSRPVPLPARPWPWAPGAE
                     RERQDDPQPLLGFTRLLSHAAQQSGRQQLGLEMAAFDAEPPQGIFSELYTSAPTLGDA
                     LRALCRYFPVSQTGTTVALTQSHGIAHLSYQIRDPAVGNPLHDAAYTLGKMARSMRRC
                     IGPSWRLDHVTLAMPAPGGADAYQRFFGAPVVCSAAYTGLHFAAEVLERPIPTADLER
                     FRNFCALLELRMPNQATPGLLADALRTWMSYAASEGRATLEHAASDFGVTPRTLQRRL
                     KEQGVGFQDLLAQVRMQTACRLLTESRLPVTCIAEQLGFSETSAFTRAFRSHARVSPR
                     AYRQAAAH"
     gene            complement(15149..16414)
                     /locus_tag="PK242_02815"
     CDS             complement(15149..16414)
                     /locus_tag="PK242_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ92983.1"
                     /translation="MSAAATPSDASLIDSRTAAWRLLVVVLLVTLGNSAMYVVSVVLP
                     AVQAEFGIGRAGASLPYTLMMVCLGLGGLWTGLWADRWGIARVLWLGAAAVGAGYLLA
                     ALSGNVWLFGLAHGLLGLLGGAATFAPLMADTGLWWHRRRGIAMAICASGNYIAGTLW
                     PPLAQWGITHYGWRPTYVALGLVCGVGMALLALAMRRRPPALSAHPASHGAAAARATP
                     WPPERPFGLTPAQAQWLLCVAGVACCVAMSMPQVHIVAYCTDLGYGAARGAEMLSLML
                     ASGIVSRLVSGWICDHIGGIRTLLLGSALQGVALLLFLPVDGLVPLYVVSALFGLFQG
                     GIVPSYAIIVREHFPAREAGARTGAVIMATLLGMALGGWLSGWVFDLTGSYRAAFLNG
                     IGWNLLNLAIASWLLWRTRGPGLRLAATP"
     gene            complement(16429..16746)
                     /locus_tag="PK242_02820"
     CDS             complement(16429..16746)
                     /locus_tag="PK242_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOZ92984.1"
                     /translation="MTTSHIANVTLTTQANVYFDGKCVSHGFQQADGSRASVGVILPA
                     TLSFNTGAAEVMECVAGGCEYRLAGETSWKSSGPGECFSIPANSSFEIRVAEVYHYIC
                     RFG"
     gene            16810..17640
                     /locus_tag="PK242_02825"
     CDS             16810..17640
                     /locus_tag="PK242_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOZ92985.1"
                     /translation="MNPPRTRFVLIETSHAGNVGAVARAMKVMGFDDLVLVRPRWPDV
                     LSRPEAIERASGAGDVLARARCVATLDEALDGMTHLCATAMTPRDFGPPTRAPRAHFD
                     LLLKQELSAHVPQAPALDFDLEAGQGVALLFGSERYGMRNEDVYRCHVALSIPTDPAY
                     GSLNLGAAVQLIAYDWRQALAARFGGFDIAPAGAAPRHADAAQLGAMLAHWEQALVAI
                     GFLDPAAPKKLMPRLQQLFNRAQPTLEEVHILRGIARAMQRAASSGQQDDGAPAPRPR
                     "
     gene            17654..18424
                     /gene="cysE"
                     /locus_tag="PK242_02830"
     CDS             17654..18424
                     /gene="cysE"
                     /locus_tag="PK242_02830"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOZ92986.1"
                     /translation="MLSRIRSYTQAILERDPAARSVWDVVFSYPGFHALVLHRLAHPL
                     WNAGWHWLARWISHWGRFLTGIEIHPGAKIGERVFIDHGMGVVIGETAEIGDDCTIYQ
                     GVTLGGTSLAKGSKRHPTLGKGVVVGASSQILGGFTVGDGAKIGSSAVVTKPVPAGAT
                     AVGNPARIILADDQTRREEAAAKLGFSAYGVTQSDDPVSQALRGLIDGTATHEHQIAL
                     LWQAVEKLACQIDPDCVPGDASRQETFEADKLNQLVGK"
     gene            complement(18447..18704)
                     /locus_tag="PK242_02835"
     CDS             complement(18447..18704)
                     /locus_tag="PK242_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HOZ92987.1"
                     /translation="MTRFTPMQTPSSPWRRLALAAALLAGLTSAGCASHPTNAQIGTG
                     VGAVVGGATGHVLFGGPVGTIGGAAAGALIGHEVGKNQRRR"
     gene            complement(18921..19514)
                     /gene="mog"
                     /locus_tag="PK242_02840"
     CDS             complement(18921..19514)
                     /gene="mog"
                     /locus_tag="PK242_02840"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HOZ92988.1"
                     /translation="MNTCDPVTIGIVSISDRASSGAYEDKGLPALQEWLGRALKNPIR
                     FEPRLIPDEQVEISRTLIELVDAGCALVLTTGGTGPAPRDVTPEATLAVAEKLMPGFG
                     EQMRQISLAFVPTAILSRQVAVIRGQSLIINLPGQPKAIAETLEGLKDADGRPQVHGI
                     FAAVPYCIDLIGGPYLETDETVCKAFRPKNAQRPVRA"
     gene            complement(19548..20228)
                     /gene="yjgA"
                     /locus_tag="PK242_02845"
     CDS             complement(19548..20228)
                     /gene="yjgA"
                     /locus_tag="PK242_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HOZ92989.1"
                     /translation="MSRKPRKGYYVRGQFVAEGSELDEELRREQLADGPSKTELKAQS
                     AELQALGEQLLSLRADLLQPLALPARLLEALQELQRITNFEGKRRQSQYVGKLMRQLP
                     GEDVAAIRAALHEQRQGAARDTLRLHAAERWRERLVADDAALSAWIGQFPDSDAQQLR
                     ALVRQARKDAAAPADAAAARARGQAARHGRAWRELFQLIQAALARAEAAGPNPDDEVP
                     YSDDTRAG"
     gene            20271..21644
                     /gene="pmbA"
                     /locus_tag="PK242_02850"
     CDS             20271..21644
                     /gene="pmbA"
                     /locus_tag="PK242_02850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HOZ92990.1"
                     /translation="MQDTSQPQAGFSYSRARFEELVDTAIAHARALGATDAGAEASEG
                     VGLSVGVRMGELETVERNRDKSLGVTVYLGQRRGNASTSDFSRAAIERTVQAAYDIAR
                     FTAEDPAAGLPDPADVAQPHEQRDLDLFHPWSIDSEQAAALALACERAALAVDRRITN
                     SEGASVSAQQSHFFSAHTHGFRGGYASSRHSLSVAPIAGRGEDMQRDYWYSSMRDAAE
                     LAAPEAVGRYAAERALSRLGSRKIATGECPVLFESPLAAGLLGSFVQAVSGGALYRRT
                     SFLLDSLGKPVFADHIDVGEDPFVRSGKGSSPFDEEGVRVQPRQVVEAGRLQGYFLST
                     YSARKLGLKTTGNAGGSHNLTLSSRRTRAGDDLDAMLRQLGTGLFVTELMGQGVNYVT
                     GDYSRGASGFWVENGRIAYPVQEITIAGNLRDMFRGIQAVGADTYTYGAKTVGSILID
                     RMTVAGN"
     gene            complement(21761..23707)
                     /locus_tag="PK242_02855"
     CDS             complement(21761..23707)
                     /locus_tag="PK242_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HOZ92991.1"
                     /translation="MTLNPLHSGWRRWCAGALLLLAPLLGWAQADPPDRVAYLSALEG
                     AAQIASDGRLLQSARLNWPVSTGTRLILDPGTRAELHGGWSAVRLAERADLSVTRLDD
                     DAVQLALTAGSLSLRVRQLQPGERIEIDTPQLALVADQPGEYRLDVDPHGDTTRLTVH
                     AGSATVYGGAGQSSRVGPHQQVSYRGRDLLVTQAAPALRDALDQWAATRDALEDRSRS
                     AQHLPRELPGYAQLDAWGEWAQDATYGAVWYPSVTAADWAPYRHGRWAWIEPWGWTWV
                     DDAPWGFAPSHYGRWTQIGHRWAWVPGRFGPRPVYAPALVAFVGGASGSLGWGVALGS
                     GAPAAAWFPLAPGEPWTPHYHASDRYRRRLNWDDERERVRPPPDSFYFQRRPGAISVA
                     PVDHFGGGGDRGRRPHFGDGSRLPPGVLNESRVVAPPPRAYVPGMATQAPLPRPGGWP
                     TAPAPRPPQAVGPGHDVAPPPGAARPPRPGDGVQRPERPLPPRTPDLPGSDRRERDRW
                     LRDPDTRGQLPVPPNDAAARQQQLQRQLQQRQEALVRERQQREAQLAPVAPAAPARAP
                     QPPMQAAPHPFERPAAPLRQPPPTESRAAPPRLPPVFESRPMPPREAGAIPPREPQRR
                     ERESEPRERPGRAPTLRSAEGMGG"
     gene            complement(23932..24387)
                     /locus_tag="PK242_02860"
     CDS             complement(23932..24387)
                     /locus_tag="PK242_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOZ92992.1"
                     /translation="MTLKDQITEDMKTAMRAKDSARVGTIRLLQAALKQKEVDERVTL
                     DDAAVIAIVDKLIKQRRDSIAAFTQAGRQDLADKEAAEMQVLQAYLPARLSADDIAAE
                     VRAIVAELGAQGPGDMGKVMGAVKARLAGKADMAAASAAVKAALSGANS"
     gene            complement(24466..>24633)
                     /gene="rpsU"
                     /locus_tag="PK242_02865"
     CDS             complement(24466..>24633)
                     /gene="rpsU"
                     /locus_tag="PK242_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOZ92993.1"
                     /translation="LRRFKRTIEKLGLLTELRAREFYEKPTAERKRKKAAAVKRHHKR
                     VRSMQLPKKLY"
BASE COUNT         3748 a         8170 c         8940 g         3775 t
ORIGIN      
        1 ccatgccttc cttgatgtcg gcgctttcgc gcaccacgtt gagctggcgc agggtttccg
       61 acatcgcttc ggccgggctc ttgggcagca cgtgctggtt gacgaagcgc ttgatggtgg
      121 tgagcaccag cggtgccgac gcggccaggg tctgagcctg ttccatggcg atttcgagct
      181 gtttgccggt cggcaccacg tcgctcacca ggccgatgcc gtaggcgcgc tgcgcgtcca
      241 tggtgcggcc cagcagcatc aggtccatgg ccgccttgtg cggaatgcgc gccgccagcc
      301 cggcgatcat gccgccggtg aagcccaggc gtgcctcggg gtaggagaac ctggtgtttt
      361 cagcggcgac gatcaggtcg cacatcatgg tcagcaccat ggcgccgccc accacccaac
      421 cggcggtggc ggcgatgatg ggcttatcgg tggtgaagcc cgagccgggg atgcagcgcc
      481 acagttcggg caggttgctg acgtcggcgc cggatgaaaa ggcgtcgttg ccggcgccgg
      541 tgatgaccgc cacgcgctgt tcggaggcgt cgaattcggc aaagccggcc tgcaactcca
      601 gcgccgtgtc cttgcagatg gcgttcttgc gcgccgggcg gttgatggtg aacagggtca
      661 ccttgccatg atgttcgatg gtgatttgcg ccatggataa ctcctgaagc tggggaatgg
      721 aaaagacggt gaatcaggcc tgggcgatgc gcgcccgcgc atccacggcc accgcgccgt
      781 ggccctgctg ccgcagcttg agggggttga cctcgatctc gaagtcgcgc ccggaagacc
      841 cggccagatc gtgcgccagc cagcccaggc gcacgatgac gtcgaccgcc gcgtccacgt
      901 ccagggcagc gcgacctcgg taggcgcgca gcagcggggc gatcttcagg ttctcgaccg
      961 cccggcgcgc gctggcggcg tcgacggggg cgggcagcac ggcgatgtcc ttgagcagct
     1021 ccaccagcac cccaccggca cctaccagca cctgggggcc gaactgcggg tcgaggcggg
     1081 cgccgacgat cagctccagt tcgcccgctt cctgtcgctg cagtgaaaag ccgtcgatgc
     1141 gggcgctggg tgcagcgcgg gcgacgctgg cctgcatcag cgccaggcgg cggcgcaggt
     1201 cgtcagggtc gaggatgttc agggccacgc cgccgacgtc ggacttgtgg gcgatgtcgg
     1261 gcgagaccac cttgagcacc agcggaaagc ccagctcggc cgccgcctct gcggcttcgt
     1321 ccgcgctgcg caccaggcgc tcgatgttga cggggatgcc agcctggccc agcagcgtct
     1381 tggacgccgc ttcgcccagg gtgccggcgg cggggaccgg cgtggcggcc atatcggccg
     1441 ggcgcaccgg cgcggccggc tcctggtaag tcgaccaggc cttccagccg cgcagcacgg
     1501 ccatggcctc ggccagcgtg tccacgtagg gcaggctgcg ctgcaccagg aactcgcgcg
     1561 cgggtgccgc cacgcggccg ggcagcatga cgtacagcgt cggcttgccg ccggccgccg
     1621 ggcggtcggc accgtcggcc atctggcgcg tcaggccggg cacgtcgggc gcggtggtca
     1681 gcaccatcag gccgaggtcc acaccggggt cggccagcgc cagctcggcc gtgacggcgc
     1741 cgatgtcgct ggtggcgtcc ggcttgcggc cgccgacgtc gatcgggttg gcgccctgac
     1801 cttcggagta gtgctcggac agcgcggcca tggtggcgtc gctgaaagtg ctcaggccga
     1861 cgccagcgcg cgagagctgg tcggccgtga tggcgccacc gccaccggag gtggtgaaga
     1921 ccgcgacgtt gcgcacctgg cgccgcggat agcgcaccat ggcccgggcc aggctcagca
     1981 tgtccagcgg gtcgtccatc aggaccacgt tctcgcgttc gcagatcgcc ttgagggcgg
     2041 cgaagtcgcc cgccagactg gcggtgtgtg agtaggcggc gcggcgcccg tcgtcggtgg
     2101 cgccggcctt gaccatcagc cagggcttgc cggcagcgcg cgcacggcgc gccagcgcga
     2161 caaagcgctg tggcgtcttg atgccctcga catagctgca gatcacctgc gtgcgttcgt
     2221 cctcgatcag gaattcgacg aaatcgttca gttccaggtc ggcctggttg cccaccgaca
     2281 cgcagtgcga gaacccgatg cccatgccgt aggagcggtc gaacagcgtg cccatcaggg
     2341 cgccgctctg gctgatgaag ccgatcggcg cctcgatcag ctgcggtacg ttgaccgccg
     2401 gcgacgagca cagcaccagc ttgttggcag ggctgatcag gcccaggcag tttggcccga
     2461 tcagtcgcat gccgtgcgcg gcggcggtgg ccaccacctc gcgctccagg gccaggcctt
     2521 cggggccggc gtcggagaac ttcgaggtga tgatgatgcc ggccttggcg ccgcgcgccg
     2581 cgcaggcggc gatctcgtcc ttgaccttgt cgcgcggcag ggccatgacc accatgtcgg
     2641 gcgcctgcgg cgtgtcggtc acgctggggt aggttttgag cccgaacagt tggtcgcggg
     2701 tcggattgat ggggtagacc gcaccgtcgt agtggtgctg caacagcgtc ttgtagaggc
     2761 ggccgccgaa cttggtctgg tcttcggacg cgccgatgac ggccaccgag cgggggttga
     2821 gcagggattt gaggctgtgg ctggaagtgg gcgaggtcac gatgtcgatc cggcaggaga
     2881 tgggaaggag ggctcagccc ttgcgctggg cggcccggta ggcggcgatt cggtcgcgca
     2941 gtccggggcg ggcggcggaa cgcaccaggc gcgccaggtc ctgggcgtct ccggcgtcac
     3001 cacgtggccg gcggccggcg gcgatggcgg cctggatggc gcgctgggtg ggcgcgtcga
     3061 gccggttcac gcgggcgccc caccgaccca gttcggcatc gatctgttcg gtctcgatgt
     3121 gccgtgtggc cagaccgcag gccagggcct ggtcggcgtc gatgcgctgg ccgccttcga
     3181 tccacgcggc ggcgtggggc gcgccgacca gttcggtcag acgcaccgtg ccgagcacca
     3241 gaccgaaggc ggcgccagga aaggcaaaac cggcgtcgcc gacgatccag cgatcgccgc
     3301 aggcggcaaa caggtcggca cccgcaccca tggtgcggcc atgcgccagc gcgagcgtgg
     3361 caaacggcgc ggcgtgcacg gcctgcagca gcagctcgac ccgtgtgaag cgcgccagca
     3421 ggctgtcgtc ggtctctcga tcgaggtccg acaggtcgaa cccggtgcag aagtgtcggc
     3481 ccgcacccga cagcaccagc aggcgcacgc tgacatcgtg agtggcggcg tccaccgctt
     3541 cggtcagcgc ctgaaccagc gcggccgaca gcgcattgcc gacctcgggc cggtgcagct
     3601 gcaggtgcag cacagggccg tcgcgccggc tcagcagttc tggctggctc atgccgcttg
     3661 cctggcccac aggttgggcg acagcgtgtt cgaatagccc gagacgaagt ccttgaccgt
     3721 gtctgtctcg ggcacgaaga cgtggcgtgc aatgcgtccg atgttgccgc cgtacaggtg
     3781 gatgctgatc gacacctggt cttccaggcc attgcgaacc acgtggatgt cgcccagcgt
     3841 cggagacacc acgtcgatgt cccccggccg cagggtttct tccgcgccgc aggccgtgac
     3901 ccgtccctgg tgatcgcgcg tgtagcgctg cgcgcattcg gcgccacgca gcatgccgat
     3961 gatgccccac accgtgtggt catggaccgg cgttttctgt ccaggccccc agacgaagct
     4021 gaccagtgaa aaacgatcca gcgggtcacc gtacagcaga tgctgctggt agtactgggg
     4081 gtgcgcagcc gccatggcgt cgggcagcca gtcgtcctgc gccaccaggg ccttcatcag
     4141 gggccgggca ccgtcgagca gcctggcctc gtcggccggc ccggactcca ccaggcgcgt
     4201 catggcggcg acaaaggtca gcaagggcga ttcggcagcc atcagttcac ctccgatcgg
     4261 gcgcattcgg cgcggatctc ttgcgtgtgc tgccccagtt cggggggcga gcggcggatc
     4321 gggaagccct gaccgttcag gcgcaccggt gaagcgaagg tgcgggtctg ctggccgttg
     4381 ggcaacgtca gcggctgcac ccagcccatg tggacggtct gcggatcggc cagcgccgcg
     4441 gcgtagtcgt tgatgcgggc gtgcggcacg ccggcttggt tgaaggcgtc gatccagtgc
     4501 gccaccggcc gctgggtgaa cacttcctcg aggatctcct tgagcgcctg ctggtttcgg
     4561 gcccggtcgg tggtgctgag gaagcggggg tcttcggcca gtgccggcat ctgcaccacg
     4621 cgcagcacgt ccagccacag cttgtggttg cccgcggcga tggcgaagta gccgtcctgc
     4681 gcctggaacg cctggtacgg cgcattgcgc gggtgggccg agcccagttt gccggggctg
     4741 cggccgttgc cgaagtattc gctggtctgc agcgcggcaa tgcccaaggt gcagccgaac
     4801 atcgggatgt cgatgtgggc accgcgcccg ccggcgcgca cgtgcgccag catggaggcg
     4861 atggaaaacg cggcatacag gccggaggcg aagtccgaca gcgggacgcc gcacttgacg
     4921 ggcgcacccg ccggttcgcc ggtgacgctc atcaccccgg ccgcggcctg gatcgtcagg
     4981 tcgaagccgc cttcgctggc gcgcggaccg ctctggccgt aggccgagat ggagcagaac
     5041 agcaactccg gtttttcggc cgacagggcg gcgtaaccca gccccaggcg atccatgacg
     5101 ccggggcggt tgttctcgac cagcacgtcc gcttccagga tcagccggcg cgccagcgca
     5161 acgtcctgcg ggtccttgag gttgagcacg gccgagcgct tgttgcggtt gagcgaggca
     5221 aagttttcgc tgtagccctg attgagtggc ggccactgac gcaggccgtc cccggtgggt
     5281 ggctcgatct tcaccacgtc ggcgcccatg tccgacagca gcatggtgga gaaggggccc
     5341 gacgcggtgg tgcagaactc caccacgcgc acaccggcca ggggcagcag cggcgcagcg
     5401 gagccgctcg cggcggcaga gtcgaaggca gatcgggtca tgtgatgcgg cgttccatga
     5461 agatggaggc gatgatagtt aattcacaaa tggaaaacta actttccaaa aaacggaacg
     5521 aagagtcggg tcgagcctgc ctcagtgcgt cagtgcggca tccagcagcc gctgtgcctc
     5581 ggggtgatgc agcgtggcgc cgcgcaagcg atcgcgtgac agcacatcgg tgaactgacc
     5641 gccgaccatg aacgcgatct ggtcgcacat gtgggccacc acatgcaggt cgtggctgac
     5701 cagcacgaac gcctgctggc gctctcggcg caggcgcgac agcaggttca gcacctcggc
     5761 ctgcaccgac acatcgagcg ccgaggtcgg ctcgtccagc agcagcacct gcggctcgac
     5821 cagcagcgcc cgggcgatgc acacccgctg gcgctgcccg cccgacagct gccctggaaa
     5881 gcggaaccgc acggcgagcg gcagggccac ggtctgcagc gcctgcacga tgcgtgcgtc
     5941 ctgatcgggc atgcggtgca cgatcagcgg ttcgcgcagg atctcgtcga tggtgtggct
     6001 ggggttgagc gcagccaggg gatcctgaaa caccatctgc atcgcccggc ggtcggccag
     6061 cgagcgctga accccgatgg gccggccggc aatctcgatc cggccggtcc actgcgtgtc
     6121 gatgccggcg acggcgcgca agatggtgct cttgccgcaa cccgattcgc cgacgatacc
     6181 caggctgccg ccatgcggaa ccgagaagga cacgccgttg accacctggg cgctgccgcg
     6241 ctgcacgatg agctggtcaa tgcgcagcgc gggcgggttc actgcagcca tgacgcctcc
     6301 cgttgcagca ccggcagatc ctgacccggg tgatcgagcg aggggcggca ggccagcaga
     6361 ccgcgcgtgt aggggtgttg cgcggcgctc agttcgccgc cgcgcagctc ttccatgacc
     6421 cgtcccgcgt acatgaccag gatccggtcc gcgtagcgcg ccaccagatc gaggtcgtgg
     6481 ctgatcagca ccagccccat gccgcgcagg cgcacctgct cctcgatcag atccagaatg
     6541 cggtggcgca ccacggcgtc aagggccgat gtggcttcgt cggcgatcag cagttcgggc
     6601 tcggccgaca gaatcatggc cagcatcgcg cgctgcgcca tgccgccgga gacttcgtgc
     6661 gggtacagac ccatgacccg cttcggatcg cggatgtgga cctcggccag cagctcgacg
     6721 gcccgctggt aggcgcccgc cgctggtacc ttgcgatgca gcctgagcat ttcgatcagc
     6781 tgcctgccaa tcgtcatcaa gggatccaga ccctgcttgg ggtcctgaac caccatgccg
     6841 atgcgccggc cccgaatggc ccgcaagtcg cgccgatcga gcgacagcag gtcctggccc
     6901 tgcaactgca tccggtcggc gcgcacgctg gcggtcgccg gcagcaggcc gaacagggag
     6961 cgaaaggtga tcgacttgcc ggagcccgac tctccgacca cggccacccg ctcacgtccc
     7021 atgcgcaggc tgatcccgcg cacggcttcg gtgcgtccgt gcgcggtctg gaagctgatg
     7081 cgcaggttgt cgatgtccag caagggaggg gcgctgtcgc gtgggatgtg gttcatgaga
     7141 agctcacttc acgccagggt cgaccacgtc acgcatggcg tcgccgacga agttgaagcc
     7201 caggctcagc cagagaatca tgatgccggg cagggtggac acccaccacg cctcgaacac
     7261 gatggcgcga ccttcggcca ccatggcgcc ccactcgggc tgcggcggcg gcaggccaac
     7321 gcccagaaag cccaggccag ccacgatcag cacgatgccc ggagcgtcca gcgccgcgcg
     7381 cacgaaggcc gatggcagac tcagcggcag cgcatgccgg aacagcaacc gcagcggtga
     7441 cacgccgatg gcgcgggccg cctgcacgaa ctcggaccgg cgcagggcgg cgatctcggc
     7501 gcgtgccacg cgggcgtagg ggggccaacc cgtggcggcg atggcgatca ccgccgtcca
     7561 caggccggcg ctgagcgcgg cggccacggc aatcgccagg atcaggcgcg ggaacgccag
     7621 aaacacgtcg gccacgcgca tcagcaccgc atcgatccag ccgccccaca gcccggcagc
     7681 ggcgcccacc agcaggccga cgggcacgct gatcaccagc accagggcga cgatggccag
     7741 cgtcggacgg gcgccgtgca ccacgcgggc gaggatgtcg cgacccagcg tgtcggtgcc
     7801 cagccagtgc gcggccgatg gtggcagcag gcgctgatcc agcgactggg cgatcggatc
     7861 ggcaagcggc agccatggcg caagcccggc ggccaacacg atcgaggcca ccatggccag
     7921 acccagcaca ccgatggggt gggtcaggaa gcggcgcgcc agccggtaga tggcaccgag
     7981 cttggcctgt gtgcgtgtgg ccggcgtatc ggaagccagc cattgccgta aggtggaaga
     8041 ggggttcatg ccagacgtga cctcggatcc agcgccgcct gaatcacttc gctggcgatg
     8101 ttgacgacca tgaagatgac gccgaccagc agcgtcgcgg tgagcaccgc cggcacatcg
     8161 gaggcgaaca gcgaatcggt gatgtacatg cccagccccg gccaggcaaa cacggtttcg
     8221 gtcagcaccg cgccttccag caggtaggcg taggtcagcg cgacgatggc cagcaccggc
     8281 ccggcggcgt tgaacagcgc atggcgccag atgatcgccg cttcggacag gcccttgaca
     8341 cgcagcaccg tggtgtagtc ctgccgcaat tgccacagca ggaagctgcg ggtcatgcgt
     8401 gcgatgtagc ccagcgcgat cagccccaac aggctggcgg gcaacaccag gtgcgagagg
     8461 gcattgcgga aggcgtccca ttcaccggcc agcacggtgt cgatcaacat caggtgcgtc
     8521 acctcgggca ccgaatagcg gtaggcggta tcgagccgcc ccggcccggc gacccactcg
     8581 agtctggcgt aaaacaccag cagaccgacg atgccgagcc agaacacggg gaccgaatag
     8641 ccgatcagag aaagcattcg gcccaggtga tcgggcaggc ggttggcgta ttgcgcgcag
     8701 acgacgccga tcggtatccc gaacaccaca cccaggatga tggccaccgt cgccagctcg
     8761 aacgtggcgg gaaagtagcg cgcgatgtcc gaaccaatgg ggttgcgcgt gaccagcgac
     8821 agtccgaagt cgccgcgcac catgtcaccc acataggcgg cgaactgctc ggtcagtggc
     8881 cggtccaggc gcagttggcg gaacacctcg tcgtactgct cctgcgaggc gcgatcgccg
     8941 acgatgcgca gcaccggatc ggccggcagc acgcgcccca gggcgaaggt gaccaccagc
     9001 agaccgaaca gcgtgatgca tgaggtcagc agggtggcac ccagccagcg caggccacgc
     9061 cgcagccgca ccgagcgcag gcgccggcgt ggggcgatgt cgacgccgtc gtcggctgct
     9121 gcagtggcca tcgggttcac cgcttctcaa tcatccagta gggcgtggag tcaaacgtga
     9181 cggcgcccga gtagttgtgc acgttggtgc gcatggcaat cggctccagc ttctggatca
     9241 ggaaggtgaa cggcgactcg tcacgcagct tgcgctggat gtcggcgtag atcttcatgc
     9301 gcttggcgtc atcgacctcg tgcgaggcct tgtccgcctc ggcggtcagc tgaggcgtca
     9361 gccagtgggc gcgccagggc gcatttttgt tgctggcctt gtccgagtca tcggtgttgc
     9421 ggctgaagaa ctccagggtc gagctggggt cggaatagtc gggcgcccag acgaacacca
     9481 gcgcgtcgtg tcgacgctca cggtagcgcg tcatcacctg agacggctcg cccatgcgca
     9541 gctccacctt gatgccgccc tgggcgaagg tcgactgcag cgactgcgac acttcgtgat
     9601 aggggctggc acccgaggtt tcgagcgaca tcgagaaccc gtttggaaag ccggccttgg
     9661 ccagcagggc cttggccttg gccggatcga gcttgtaggg ctcttcggtc agggcgccgg
     9721 gggtgcccct ggccacggcc gactggttga cccggtactg gccggaaaac agtttgttgg
     9781 caatgccgtc gtagtcgatc agccagcgca tcgcctgcca gacttcgggt ttggacaggt
     9841 tggggttggc cgtgttgagg gccaggtagt agacggtgcc acgcggaatc tgaatcatct
     9901 tcaggttggc gttgcccttg atcgactgca gctggtctgc cttcaggtcg gaggccacgt
     9961 ccacatcgcc cttttccagc agcaggcgct gcgtcgcagg ctcggcgatg tgtcgaagga
    10021 tgacgcgctt gagcttggcc ggtgagccgc ccttgaagcc cgggaaggcc tccagcacca
    10081 ccacgtcctt ggccttccat tccaccagct tgtaggcgcc ggcgcctgcc gatcgggtgc
    10141 gcagccagcc gttgcccaga tcgccgttgc gttcgttgga catcaccagc ttcatgtcca
    10201 ccaccgaagc gatggcggcg ctgagcgcgc tgaggatcag ctcggtcgag tagggctggt
    10261 caaagctcag caccagcttg tcgccatcgg cgcgtacccg ctgcttgacg ttgtccttgg
    10321 tccagccgaa ctggcgcagg atgatggcgg gttgcttgtc cagcaggatg ccgcgctgca
    10381 gcgagaacgc ggcgtcttcg gccgtcaccg ggttgcccga ctggaacttc agatccttgc
    10441 ggatctggaa cacgatctgc ttgccgtcgg gtgcggcctg ccagctctgc gccacgcccg
    10501 ggacgatctt ggaaaaatcg gcgggatcgt gctgcaccag gcgcaggtag aggctgttga
    10561 gtgcgtcgcc cggggcgatt tcaaacgcct cctggggatc caggctgagg atggtgccaa
    10621 tgtcgcgggc gatgaccagc gtgtcggcgg gggtggctgc ccaggtcgcc gtagcgctca
    10681 ggcaggcggc cagtacggag gtgctcagaa cttgtctcat cgattttttc atggggaagg
    10741 tcctctggtg gatggagtca gggtgtgaca tcgcgtcggt gttggtcgca ggcaagcggg
    10801 tcagtccatc tgggtcacga acttggtgac caggtagccg tcaaaggttt cgctgccgcc
    10861 ttcgctgccc atgccgctgt ccttgatgcc gccaaacggc gtttcaggca gcgcctggcc
    10921 gaagtggttg atgttgacca tgccggcctc caggccgttc tggatctgca gggcgtggcg
    10981 cgtgttgctg gtgaaggcgt aggacgacag gccgaacggc aggctgttgg cgcgctggag
    11041 ggcctcgtcc aggtccttga acgggacgca ggcggcaatg gggccaaacg gctcggcctg
    11101 catgatgcgg gcgtcgtcgg gtggcgcggc aatcacggtc ggagcgaaga agttgccgac
    11161 ctcgcccagc cgcgtgccgc cggtttcgat gcgggcgccg cgcgcggcag cgtcttcgac
    11221 gaactcggcc atcgccgtca cgcggcgcgc gtgggccagc ggtcccatgc gcgtgtccgc
    11281 ctccagtccc ggcccgacct tgatctggcg gctgagttcg gtgaagctgg ccatgaagcg
    11341 atcccagacc ggctcctgga tgtagaagcg cgtgggcgag acgcagacct gcccggcgtt
    11401 ggtgaacttg gacgcggcca gcatggtggc cgcccgctcg acgtcggcat cggcgcacac
    11461 gatgaccggc gaatggccgc ccagctccat cgtgatgcgc ttcatgtggc tgcccgccag
    11521 ggcggccagc tgcttgccca ccggcaccga gccggtgaag gacaccttgc gcacgatcgg
    11581 tgaggcgatc aggtgctccg atacctcatg gggcacgccc cacaccacgt tcagcacgcc
    11641 ggggggcagc ccggcttcgt ggaacagccg cgcaatggcc accacggcac tcggggcatc
    11701 ctcggcaccc ttgatgacga tggtgcagcc cgccccgatg gccgcggcga tcttgcggat
    11761 cgcctggttg tagggaaagt tccagggcgt gaaggcggcg cacacgccga tcggctcgcg
    11821 cagcacgaac tgccgcacgt tcggcgtgcg cggcggaatc acgcggccat agatgcggcg
    11881 gcactcctcg gcgtgccagt cgcaatggtc ggcgcagcgc agcacttcgc ccacagcctc
    11941 ggccagtggt ttgccctgat cgagcgtgat gttgcggccg atgtccttgg cgcgttcacg
    12001 gctgagctga ccgaccttgc gcaggatggc gctgcgctcc atcggcgaga catggcgcca
    12061 ggtggcgaag gcgcgctcgg ccgccgccag cgcgcgatcc agatcggccc gggtcgcatg
    12121 cggcagctgg ccgatggtgt tgccggtggc ggggtccagc acgggttgct cgacccggcc
    12181 gccgccctgg atgaattcgc cgtcgatgta tagggccaag gattcgtaca tgagggtttg
    12241 cgctccggtt cgatgcttcc aatgcgggtg tctgtcggtc aggaggtggc gaggcttgcg
    12301 ccgaactgca cctgggctgc gtcgacggct cgggtcagcc cgtccaccag ctcgtcgatg
    12361 tgccggcgtt cgctgatcag tggtgggcac agtgcaatgc agttggcgat ggcgcgcacg
    12421 atcacgccgt gttccatcgc cttgttctga atccatgcgc cgaccgcctt gtcgggcgta
    12481 aaaggggttt tgctggcttt gtcggccgtg atttcgacgg ccgccagcag gcccatgccc
    12541 cgcacttcac ccaccagcgg gtggtcggcc agaccctgga gtcgctggaa gaaatagggc
    12601 gacagggcct gtacatgttc gacgatcctg cgttcctggt agagcctgat ggtttcgagc
    12661 gccaccgcag cggccaccgg gtgggccgcg taggtcaggc cgtgggcaaa ggcgcccagc
    12721 ttgcggctgc cggccttcag ggcttcgtgg atctggtcgg tgatcagcag gccggagatc
    12781 ggctggtagg cgcccgacag gcccttggcc acggtcatca tgtccggctt gagctggaag
    12841 ctgtcgctgc cgaacatctg gcccgtgcga ccgaaaccgc agatcacctc gtcgacgatg
    12901 aacaggatgt cgtatttctt cagaacggct tgcacgcgat cgaaatagcc ggccggcggc
    12961 acgatcacgc cgccggaacc gttgatgggc tcggcgatga aagccgccac cgtgtccggg
    13021 ccttccttga ggatcaactg ctccaggttg ttggccaggc gttcggcaaa ctgcgcttcg
    13081 gtttcgctcg gcagcgcgaa gtggtagtgg ctggggcagt cggtgtgcag cacctgcggg
    13141 atcggcaggt cgaaatcgcg gtgcacgtgc accagaccgg tcaggctgcc cgaggccacg
    13201 gtgacgccgt ggtagccgtt gatgcgcgag atgatctttt tcttgctggg tcggccgagc
    13261 tggttgttgt agtaccagac cagcttgacc gctgcgtcgt tggcttcgga gcccgagttg
    13321 gcgaacagca catggttgag ccccgccggc gcgatggcgg tgagcgcctc ggccagggcg
    13381 atgccgggcg ggtgtgagcg atggttgaac aggtggtagt agggcagggt gcgaaactgc
    13441 gccatcgcgg cgtcaatcag gcgctgttcg gaaaacccca gtgaagcgca ccagaggccg
    13501 gacatgcctt ccaggtaacg cttgccgttt tcatcctcga catagcagcc gtcaccgcgc
    13561 gtgatgacca cggggccgcg ccggtcgtgg gcgtcgaggt tggagtaagg atgcacgacg
    13621 tgatcgatat ccttttgcca gttggggctg ggggtgctgc tcatgaggtg gccttgacgt
    13681 tggggttcaa tcggatgcca ggtcccgaag cgagcggctt acgcgcgaag ctgtggctcg
    13741 tgggagcgga cttgcagcgt accccgcgag cgccggcagg acttgaccga tcacgacaat
    13801 cgatcccctt gcggcggtcg atgctgctca gtgggccgcg gcctggcggt acgcgcgtgg
    13861 gctgacgcgc gcatggctgc ggaaggcgcg cgtgaaggcg ctggtttcgc tgaagcccag
    13921 ttgttcggca atgcaggtga cgggcagccg gctttccgtc agcaggcggc acgcggtctg
    13981 catgcgcacc tgcgctagca ggtcctgaaa accgacgccc tgttccttca gccgccgctg
    14041 cagcgtgcgc ggcgtgacgc cgaagtcgct ggcggcgtgc tccagcgtgg cccgcccctc
    14101 gctggcggca tagctcatcc aggtccgcaa ggcgtcggcg agcaggcctg gtgtggcctg
    14161 gttcggcatg cgcagctcca gcagggcgca gaaattgcga aatcgttcga ggtcggcggt
    14221 cgggatgggg cgctccagca cctcggccgc gaaatgcagg ccggtgtatg ccgcactgca
    14281 aaccacgggc gcgccgaaga agcgctgata ggcgtcggcg ccgccggggg ccggcatcgc
    14341 cagcgtgacg tgatcgagcc gccaggatgg accgatgcag cggcgcatgg agcgcgccat
    14401 cttgcccagg gtgtaggcgg catcgtgcaa cgggttgccg accgccggat cgcggatctg
    14461 gtaggacaga tgggcgatgc cgtgagactg cgtcagcgcc accgtggtgc cggtctggct
    14521 gaccgggaag tagcggcaaa gggcgcgcag cgcgtcgccc agcgtggggg cggaggtgta
    14581 aagctcgctg aagatgccct ggggcggctc ggcatcgaag gcggccattt ccaggcccag
    14641 ttgctggcgg ccactttgct gcgcggcgtg gctcagcagg cgcgtgaagc ccagcaaggg
    14701 ctgcgggtca tcctggcgct cgcgttcggc gcctggcgcc cagggccagg gcctcgcggg
    14761 caggggcacc gggcggctca acaggcggtg caggacgtgc gtggccaggc gagtggcctc
    14821 accgctgatg gcgccaacca gaagtcttgt ggtcatgggc gggcgtccga cctgggaagt
    14881 gcattgaatt ccgtaaaatg gaaagtgaat tttaatatag caaattaacc aaaaccatta
    14941 gggtgaaccc cggcgtgcag cgggtggctt gggcgctgtg gatcgctcgg gcgggttcat
    15001 ggggcgtgcc ggcagcgggt cgatggccgc agcttgtgcc tgccgggggg gcgccgtcaa
    15061 gtggcaacgc gccgccgccc ggcatgcgcg gggtgggcgg ggggctgcgc ggtgcccgca
    15121 gcgcccgatc gcaggcgctg gcgtgcgatc agggtgtggc cgccagccgc aggccgggcc
    15181 cgcgcgtgcg ccacagcagc cagctggcaa tggccaggtt cagcaggttc cagccgatgc
    15241 cgttgaggaa ggcggcgcgg tagctgccgg tcaggtcgaa cacccagccg ctcagccagc
    15301 ctcccagcgc catgcccagc agcgtggcca tgatgaccgc gccggtgcgg gcgccggctt
    15361 cgcgtgccgg aaaatgctcg cgcacgatga tggcgtagga cggcacgatg ccgccctgaa
    15421 acagcccgaa cagggccgac accacgtaca gcggcaccag cccgtcgacc ggcaggaaca
    15481 gcagcagcgc cacgccctgc agggccgaac ccagcagcag cgtgcggatg ccgccgatgt
    15541 ggtcgcagat ccagcccgac accaggcggc tgacgatgcc gctggcgagc atcagcgaca
    15601 gcatctcggc gccgcgcgcc gcgccgtagc ccaggtcggt gcaataggcc acgatgtgca
    15661 cctgcggcat cgacatggcc acgcagcagg ccacgccggc cacgcacagc agccattggg
    15721 cctgtgccgg cgtcaggccg aacgggcgtt cggggggcca gggcgtggcg cgtgccgcgg
    15781 cggcgccgtg gctggccgga tgggccgaca gcgccggcgg gcgccgccgc atggccagcg
    15841 ccagcagcgc catgccgacg ccacacacca ggcccagcgc cacgtaggtg ggccgccagc
    15901 cgtagtgggt gatgccccat tgcgccagcg gcggccacag cgtgccggcg atgtaattgc
    15961 cactggcgca gatggccatg gcgatgccgc gtcgccggtg ccaccacagg ccggtgtcgg
    16021 ccatcagcgg cgcgaaggtg gcggccccgc ccagcaggcc cagcaggccg tgcgccagac
    16081 cgaacagcca cacgttgccc gacagcgccg ccagcaggta accggcgccg accgccgccg
    16141 cgcccagcca cagcacgcgc gcgatgcccc agcggtcggc ccacaggccg gtccacaagc
    16201 cgcccagccc caggcagacc atcatcaggg tgtagggcag cgaggcgccg gcgcggccga
    16261 tgccgaattc ggcctgcacc gccggcagca ccaccgacac cacgtacatg gcgctgttgc
    16321 ccagcgtcac cagcaacacc accaccagca ggcgccaggc ggcggtgcgc gagtcgatca
    16381 ggctggcgtc ggacggcgtg gcggcggcgg acatggttgc ctgcgcggtc agccgaagcg
    16441 gcagatgtag tgatagacct cggcaacgcg gatctcgaag ctggagttgg ccgggatgct
    16501 gaagcactcg cccggcccgg acgacttcca ggacgtttcg ccggccagac ggtattcgca
    16561 gccgccggcc acgcattcca tgacttcggc cgcgccggtg ttgaagctga gggtggcggg
    16621 caggatcacg cccacgctgg cccggctgcc gtcggcctgc tgaaagccgt gcgagacgca
    16681 cttgccgtcg aagtagacgt tggcctgggt ggtgagggtg acgttggcga tgtgcgaggt
    16741 ggtcatggat cggtgacagg gttgcaggaa caagccgcgc atgttatcga ggcggcgaca
    16801 atcctgccca tgaacccccc gcgcacccgc ttcgtgctga tcgaaaccag ccacgccggc
    16861 aacgtgggcg cggtggcgcg cgccatgaag gtgatgggtt tcgacgacct ggtgctggtg
    16921 cgaccccgct ggcccgatgt gctgtcgcgt cccgaggcga tcgagcgcgc cagcggcgcc
    16981 ggcgacgtgc tggcgcgcgc gcgctgtgtg gccacgctgg acgaggcgct cgacggcatg
    17041 acgcacctgt gcgccacggc gatgacgccg cgcgacttcg ggccgcccac gcgggcgccg
    17101 cgcgcgcact tcgatttgct attgaaacag gagctgtcgg cgcacgtccc gcaagcgcca
    17161 gcgctcgatt ttgatcttga agcggggcag ggcgtggcgc tgctgttcgg ctccgaacgc
    17221 tacggcatgc gcaacgagga cgtgtaccgc tgtcatgtcg cgctcagcat cccgaccgat
    17281 ccggcctacg gctcgctcaa cctcggcgcc gccgtgcagc tgatcgccta cgactggcgt
    17341 caggcgctgg cggcgcgctt cggcggcttc gacatcgcgc cggccggcgc cgcgccgcgc
    17401 cacgccgacg cggcgcagct gggcgccatg ctggcgcact gggagcaggc gctggtggcc
    17461 atcggctttc tggatccggc cgcgcccaag aagctgatgc cgcgcctgca gcagctgttc
    17521 aaccgcgccc agccgacgct ggaagaggtg cacatcctgc gcggcatcgc ccgcgccatg
    17581 cagcgggcgg cgtcgtccgg ccagcaggac gacggcgcgc cggcgccgcg tccgcgctag
    17641 acttcgcacc ccgatgttgt cccgcatccg ttcctacacc caggccatcc tggagcgcga
    17701 tcccgccgcc cgcagcgtgt gggacgtggt gttttcgtac ccgggctttc atgccctggt
    17761 gctgcaccgg ctggcccacc cgctgtggaa cgccggctgg cactggctgg cgcgctggat
    17821 ctcgcactgg gggcgcttcc tgaccggcat cgagatccac ccgggcgcca agatcggcga
    17881 gcgtgtgttc atcgatcacg gcatgggtgt ggtgattggc gagaccgccg agatcggcga
    17941 cgactgcacc atctaccagg gcgtcacgct gggcggcacc tcgctggcca agggcagcaa
    18001 gcgccacccg acgctgggca agggcgtggt ggtgggggcg tccagccaga ttctgggcgg
    18061 cttcaccgtg ggcgacggcg ccaagatcgg ctccagcgca gtggtcacca agccggtgcc
    18121 ggcgggcgcc acggcggtgg gcaacccggc gcgcatcatc ctggccgacg accagacgcg
    18181 gcgcgaggaa gccgcggcca agctgggctt ctcggcctac ggcgtgacgc agagcgacga
    18241 cccggtgagc caggcgctgc gcggtctgat cgacggcacc gccacgcacg agcaccagat
    18301 cgccctgctg tggcaggcgg tggaaaagct ggcctgccag atcgacccgg actgcgtgcc
    18361 gggcgatgcc tcgcggcagg aaaccttcga ggccgacaag ctcaaccagc tggtcggcaa
    18421 gtgacggtgc gccgcaggcg cgggcctcag cgccggcgct ggttcttgcc gacttcgtga
    18481 ccgatcaggg cgccggcggc ggcgccaccg atggtgccca cagggccgcc gaacagcaca
    18541 tggccggtgg caccgcctac caccgcaccg acgccggtgc cgatctgggc gttggtcggg
    18601 tggctggcgc agccggcgct ggtcaggccg gccagcaggg cggcggcgag cgccaggcgg
    18661 cgccaggggg acgatggggt ctgcatgggg gtgaagcgtg tcatggcggg catgatcgcc
    18721 cgcatccccc gttggggtgt gacaaacccg gcgcacttgc aaccagaggt cgcacggcgt
    18781 gggtggcgcg gttacgatgg gagaccgatt ttgcggtgtt tccgggctcc agcgcttgac
    18841 tgacctgcgc cagcagctcc cgattcaata gcacatatgg tattgggcca gcgcccggcc
    18901 cggcgtggac ggcagcggcc tcaggcccgg accgggcgct gcgcgttctt gggccggaag
    18961 gccttacaga cggtctcgtc ggtttccagg taggggccgc cgatcaggtc gatgcagtag
    19021 ggcacggcgg caaagatgcc atgcacctgc ggccggccgt cggcgtcctt cagcccttcc
    19081 agcgtctcgg cgatggcctt gggctgcccg ggcaggttga tgatcaggct ctggccgcgg
    19141 atcaccgcca cctgacggct caggatggcg gtcggcacga aggccaggct gatctggcgc
    19201 atctgctcgc caaagccggg catgagcttt tcggccaccg ccagcgtggc ctcgggcgtg
    19261 acgtcgcgcg gcgccgggcc ggtgccgccg gtggtcagca ccagggcgca gccggcgtcc
    19321 accagctcga tcagggtgcg gctgatttcc acctgctcgt cggggatcag gcgcggttcg
    19381 aaacggatcg gattcttcag cgcccggccc agccattcct gcagcgcggg cagccccttg
    19441 tcttcatagg cgccgctgga ggcgcggtcg ctgatggaga cgatgccgat ggtgacgggg
    19501 tcgcaggtgt tcatcgaagt tgctatgtga atggtagctg gtggcgctca gccggcgcgg
    19561 gtgtcgtcgc tgtagggcac ttcatcgtca gggttggggc cggccgcttc ggcacgcgcc
    19621 agcgccgcct ggatgagctg gaacagctcg cgccaggcac gtccgtggcg cgctgcctgg
    19681 ccgcgggcgc gcgccgcggc ggcgtcggcg ggcgcggcgg cgtccttgcg cgcctgacgc
    19741 accagcgcgc gcagctgctg cgcatcgctg tccggaaact ggccgatcca ggcgctcagc
    19801 gcggcgtcgt cggcgaccag gcgctcgcgc cagcgctcgg cggcgtgcag gcgcagggtg
    19861 tcgcgcgcgg cgccctggcg ctgttcgtgc agcgcggcgc ggatggcggc cacgtcttcg
    19921 cccggcagct ggcgcatcag cttgccgacg tactggctct ggcgccgctt gccctcgaag
    19981 ttggtgatgc gctgcagctc ctgcagcgcc tccagcagcc gcgcaggcag cgccagcggc
    20041 tgcagcaggt cggcgcgcag gctcagcaac tgctcgccca gggcctgcaa ctcggcgctc
    20101 tgggccttca gctcggtctt gctggggccg tcggcgagct gctcgcgccg cagttcttcg
    20161 tccagctcgc tgccttcggc cacgaactgg ccgcgcacgt aatagccttt tctgggtttg
    20221 cgagacatgg gtgaagcggt caaaagtcgg ccagtatgat agccgccacc atgcaagaca
    20281 cctcccagcc ccaggcgggc ttttcctatt cgcgcgcccg gttcgaggag ctggtggaca
    20341 cggccatagc ccacgccagg gcgctgggcg ccaccgacgc cggtgccgaa gcgtccgagg
    20401 gcgtgggcct gagtgtgggc gtgcgcatgg gcgagctcga gacggtcgag cgcaaccgcg
    20461 acaagtcgct gggcgtgacg gtctacctgg gccagcggcg cggcaacgcc agcacctcgg
    20521 acttttcacg cgccgccatc gagcgcaccg tgcaggcggc gtacgacatc gcgcggttca
    20581 ccgccgaaga tccggccgcc ggcctgcccg atccggccga tgtggcccag ccgcacgagc
    20641 agcgcgatct ggacctgttt catccctgga gcatcgacag cgaacaggcg gcggcgctgg
    20701 cgctggcctg cgagcgcgcc gccctggcgg tggaccggcg catcaccaac agcgaaggcg
    20761 ccagcgtgtc ggcgcagcaa agccacttct tcagcgccca cacgcatggc tttcgcggcg
    20821 gctacgccag ctcgcgccac agcctttcgg tggcgccgat cgccggcagg ggcgaggaca
    20881 tgcagcgcga ctactggtac agctcgatgc gcgacgccgc cgagctggcc gcgcccgagg
    20941 ccgtgggccg ctacgccgcc gagcgggcgc tgagccgcct gggcagccgc aagatcgcca
    21001 ccggcgaatg tccggtgctg ttcgagtcgc cgctggcggc cggcctgctg ggcagcttcg
    21061 tgcaggcggt cagtggcggc gcgctgtacc ggcgcacctc gttcctgctg gattcgctgg
    21121 gcaagccggt gtttgccgac cacatcgacg ttggcgaaga cccgttcgtg cgcagcggca
    21181 agggctcctc gccgttcgac gaggaaggcg tgcgcgtgca gccgcgccag gtggtcgaag
    21241 cgggccgcct gcagggctac tttctcagca cctattcggc gcgcaagctg ggcctgaaga
    21301 ccaccggcaa cgccggcggc tcgcacaacc tgacgctgag ctcgcgccgc acccgggccg
    21361 gcgacgatct ggacgccatg ctgcgccagc tcggcaccgg cctgttcgtc accgagctga
    21421 tgggccaggg cgtgaactac gtcaccggcg actattcgcg tggcgccagc ggcttctggg
    21481 tcgaaaacgg ccgcatcgcc tacccggtgc aggaaatcac catcgccggc aacctgcgcg
    21541 acatgttcag gggcatccag gccgtcgggg ccgacaccta cacctacggt gccaagaccg
    21601 tcgggtcgat cctgatcgac cgcatgactg tggcgggcaa ctgatcctgt ggctttgaac
    21661 cgttcaacca gcgcttgacc catgcgcctg ccgtcatgca ggcgcagcgc ggtcatgggt
    21721 catccgtcat ggggcgcaac gcgttttcgc gctgcgccgc tcagccgccc atgccttccg
    21781 cagaacgcag ggtgggcgcc cgcccggggc gttcgcgggg ctcggactcg cgctcacggc
    21841 gctgcggttc gcgcggcggg atggcacccg cctcgcgcgg cggcatcggt cgagactcga
    21901 acaccggcgg caggcgcggc ggcgccgcgc ggctttccgt cggcggcggc tgccgcagcg
    21961 gcgcggcggg gcgctcgaac gggtgcggcg cggcctgcat gggcggctgc ggcgcccggg
    22021 ccggggcggc gggcgcgact ggcgccagct gcgcttcacg ctgctgacgc tcgcgcacca
    22081 gggcttcctg gcgctgctgc aactgccgct gcagctgctg ttggcgcgcc gcggcatcgt
    22141 tcggcggcac cggcagctgg ccacgggtgt cgggatcgcg cagccagcgg tcgcgctcgc
    22201 ggcggtcgct gcccggcaga tcgggcgtgc gcggcggcag cggccgttcg ggccgctgga
    22261 cgccatcgcc ggggcggggc gggcgtgcgg ccccgggtgg cggcgccacg tcgtgacccg
    22321 ggccgacggc ctgcgggggg cggggggcgg gtgcggtggg ccacccgccg gggcgcggca
    22381 gcggcgcctg cgtggccatg cccgggacgt aggcgcgcgg cggcggcgcc accacacggc
    22441 tttcgttcag cacgcccggg ggcaggcggc tgccgtcgcc gaaatggggc cggcgaccgc
    22501 gatctccgcc accgccgaaa tggtcgaccg gcgccacgct gatggcgccc ggccggcgct
    22561 ggaagtagaa gctgtccggc ggcggccgca cgcgttcgcg ctcgtcgtcc cagttgaggc
    22621 ggcggcggta gcggtcgctg gcatggtagt gcggcgtcca gggctcgccc ggggcgagcg
    22681 ggaaccaggc cgccgccggt gcgccgctgc ccagcgctac accccagccc aggctgccgc
    22741 tggcgccacc gacgaaggcc accagcgccg gcgcgtatac cgggcgcggc ccgaagcggc
    22801 cgggcaccca ggcccagcgg tggccgatct gggtccagcg gccgtagtgc gagggggcaa
    22861 aaccccaggg cgcgtcgtcc acccaggtcc agccccaggg ctcgatccag gcccagcggc
    22921 cgtggcggta gggcgcccag tcggccgcgg tgacgctggg gtaccagacg gcgccgtagg
    22981 tggcgtcctg cgcccattca ccccaggcgt cgagctgggc gtagccgggc agctcgcgcg
    23041 gcaggtgctg ggccgagcgc gaacggtctt ccagcgcgtc gcgcgtggcg gcccactggt
    23101 cgagcgcatc gcgcagcgcc ggcgccgcct gggtgacgag cagatcgcgc ccgcggtagc
    23161 tcacctgctg gtgcgggccg acccgggacg actgtccggc gccgccgtac acggtggcgc
    23221 tgccggcatg caccgtcagg cgcgtggtgt cgccatgcgg gtcgacgtcc agccggtatt
    23281 cgcccggctg gtcggccacc agcgccagtt gcggggtgtc gatctcgatg cgctcacccg
    23341 gctgcagctg gcgcacacgc agcgacaggc tgccggcggt gagcgccagc tggacggcgt
    23401 cgtcgtcgag ccgggtgacg ctcaggtcgg cacgctcggc cagacgcacg gcgctccagc
    23461 cgccgtgcag ctcggcgcgc gtgccggggt cgaggatgag acgggtgccg gtgctcaccg
    23521 gccagttcag gcgggcgctc tgcagcaggc ggccgtcgct ggcgatctgc gccgcgcctt
    23581 ccagtgcgct gaggtaggcc acgcggtccg gcgggtcggc ctgcgcccag ccgagcagcg
    23641 gtgccagcag cagcaacgcg ccggcgcacc agcggcgcca gccggagtga agggggttca
    23701 gtgtcatggc ctgcatcggg ccggcgcagg aagcctgggc tgcaccggct ccgattcaac
    23761 gcgccggcat ggcgggcggt ttgccgcgcc atgtgtcggg cgtgcaaagt cgggggtgcc
    23821 gtttgcgtcc aaatgtgacg accggcccag ccggggggcg ctgcccggcc cttggtttgt
    23881 gagacggatc ggccgctggc gcttgccggg agagcgccag cagctattgt ttcaggagtt
    23941 tgcgccgctc agggcggctt tgaccgcggc gctcgccgca gccatgtcgg ccttgcccgc
    24001 cagccgcgcc ttgacggcgc ccatgacctt gcccatgtcg cccgggcctt gggcgcccag
    24061 ttcggccacg atggcgcgca cctcggcggc gatgtcgtcg gccgacaggc gcgccggcag
    24121 gtaggcctgc agcacctgca tttccgcggc ttccttgtcg gccaggtcct gccggccggc
    24181 ctgggtgaag gcggcgatgc tgtccctgcg ctgcttgatc agcttgtcga cgatggcgat
    24241 gacggcggcg tcgtccaggg tcacgcgctc atcgacctcc ttctgcttga gcgccgcctg
    24301 cagcaggcgg atggtgccca cccgcgcact gtccttggcg cgcatggcgg tcttcatgtc
    24361 ttcggtgatc tggtccttga gggtcatggc ggtggtccgg acgaaaagca aaaaggcccg
    24421 ctgagcgtcc ccggcgagcc tttgaagggt ggcgcaagaa gccgatcagt acagcttctt
    24481 gggcagctgc atgctgcgca cgcgcttgtg gtggcgcttg acggcggcgg ccttcttgcg
    24541 cttgcgctcg gcggtgggct tttcgtagaa ctcgcgggcg cgcaactcgg tcagcaggcc
    24601 cagcttttcg atggtgcgct tgaagcggcg cag
//
LOCUS       DAOOOG010000018        24407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_1940_length_24407_cov_26.980371, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000018 DAOOOG010000000
VERSION     DAOOOG010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 24407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24407
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1940_length_24407_cov_26.980371"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="PK242_02870"
     CDS             complement(<1..1299)
                     /locus_tag="PK242_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOZ92994.1"
                     /translation="MPDPVCNLYRQAKMNAPAYEHNGQPCTREQFYAVACDPRRSVAV
                     EACAGAGKTWVLVSRILRALLAGSASHEILAITFTKKAAGEMRERLQQWLADFARPRA
                     DESPAQWQARLDAELIARGIEPQRLPDQRKQLSNLYQNLLAQGRPVQIRTFHSWFAAL
                     LQSVPLALLDDLGLPLRYELLEDDEDAVAEVWRPFLRAVDGDAALRADYLALVAELGR
                     HNAHQALEGALARRVEFALADAAGAVDEAVASAASLYPQFAGFDDPTDNLLTDAAHAR
                     WHAWSSALGVEKNKTPRNAADAVVQAFLLESDGAARLARLRKAFFVADEDRLTQHLKA
                     YPAAQQAEEELLALLAAEQQHNARQHHQRMVRLTRALIDCFAELKRARGWIDIPDIER
                     AALQLLANHELSAWVQQRLDARIRHLLIDEFQDTNPLQWQA"
     gene            complement(1433..3988)
                     /locus_tag="PK242_02875"
     CDS             complement(1433..3988)
                     /locus_tag="PK242_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOZ92995.1"
                     /translation="MNAIAVGDEPLGAAAAPAPWRALLAEVRQQLHQLAAHPARSVVL
                     LPYAQLLPLAARLWAAQFPDSFAPRFETTRSWAERAGLFVPGPHDLVFDPARDLLTAA
                     TLLEGAGLASQRTALAGPLLEQASLLGRLAASLPPALRPDWAERARAALPAAASGPLA
                     LESRLAHIAIAWAAHSDYASDVLFAPRTTAALDALVIVPGLQTDALTTHLAEHLSEKA
                     VLLAFDAPAPGAAVALHRCLDAEDEAERAAACVLRHLHAGRTPVALVATDRGLTRRIN
                     ALLAARGVQAGAALHDETGWRLSTTHAGAALMAALRACAPAASTDAVLDWLKLAPAFD
                     GGAVAALEHALRQRAVRGWAQAAQITQGQPLTTQIEALRAPLAAPRPLTRWLADVRAL
                     LQGCGLWPLLAQDAAGSALIEALALDDAHGSAWRDWPPAQRRMGLGEFTRWVSEALEA
                     ASYRAPQIGAPAVVVLPLAQLLARPFAAVVLPGADEQRLPAAPEPPGPWSDAQRAALH
                     LPTRADLQREQAAAWVLALGAPQLDVLWRHGDDSGQSLLASPLVLALDFALNPGASAG
                     VVDAPDPRTPRSVAAAPTERPRPSGAPLPTQPLSASRYQQLRECPYRFFALRQLGLQG
                     DDELDLDVDKRDWGIWLHAVLAAFHTALAASPGADRVALIEQAAQQVTAQTLDGSFEP
                     SEFMPFAAAWPALRDAYLNWLADHEADGFAFAAAEVERSGPCGTLQLRGRIDRIDRDA
                     HGAPLLIDYKTERGEKTSARVKAGSEDTQLPFYALLSGLDAPRAAYLNLPERGEPKLH
                     ELPDLAERAAQLYEGIERDMTQIAAGAPLPALGEGSVCDWCEVRGLCRKDFWG"
     gene            4090..4422
                     /gene="trxA"
                     /locus_tag="PK242_02880"
     CDS             4090..4422
                     /gene="trxA"
                     /locus_tag="PK242_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HOZ92996.1"
                     /translation="MASDLIKHVSDSSFEADVLKSDKPVLVDFWAEWCGPCRAIAPTL
                     DELAGAYDGKLQIAKINVDDNRAVPAKFGIRGIPTLMVFKNGQLAATKVGALTKAQLT
                     QFIDQQLA"
     gene            4669..5934
                     /gene="rho"
                     /locus_tag="PK242_02885"
     CDS             4669..5934
                     /gene="rho"
                     /locus_tag="PK242_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOZ92997.1"
                     /translation="MHLNELKVLHVSELLKQAEALEIDNASRMRKQELMFAIIKKRAK
                     GGEQVFADGVLEILPDGFGFLRSPDTSYTASTDDIYISPSQVRRFNLHTGDMIEGEVR
                     IPKDGERYFALTKLDMVNGGPAEQNKHKVMFENLTPLFPREQMRLERENFKGEENITG
                     RIIDVIAPIGKGQRALIVAPPKSGKTVMMQTIAHAISANYPDSHMMVLLVDERPEEVT
                     DMQRSVKGEVIASTFDEPAARHVHVAEMVIERAKRLVELKKDVVILLDSITRLARAYN
                     NVVPSSGKVLTGGVDANALHRPKRFLGAARNVEEGGSLTIIATALIDTGSRMDEVIFE
                     EFKGTGNSEIHLDRRLYEKRVFPSIQLNRSGTRREELLLAPEVLQKTRILRQFMYNMD
                     EIEAMEMVIKSMKATKTNVEFFDMMRRGG"
     gene            6014..6268
                     /locus_tag="PK242_02890"
     CDS             6014..6268
                     /locus_tag="PK242_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOZ92998.1"
                     /translation="MREGIHPNYREVCFVDLSNGFKFVTRSCVNTKETVKMDDGRELP
                     LFKLDTSSESHPFYTGTQKSVDNMGGRVEKFRNRYGKVAR"
     gene            6360..8069
                     /locus_tag="PK242_02895"
     CDS             6360..8069
                     /locus_tag="PK242_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ92999.1"
                     /translation="MNRPSPAIVTQDAVRRLPRWALLLLCVAYVIPGYVGRAPWKNAD
                     LASFGYMLALRDAGDGQAWLHPTLMGVAPDSPALLPYWLGAWAMRWLQPLGLAPDVAV
                     RLPFVLLLAGTLVASWSAMYLLARHPSAQPVAFAFGGEAEPRDYARALADGSLLALIA
                     TLGLAQFSHETTPVLAQLFFGTLVFYGLAALATRPRSAGLALACGLPGLTLSGAPTTA
                     LLYAGLSALALALPGPTHVPAAVRRGALWLLPALMAACLALALTLGLWPTELPAWRSR
                     WSDWRSLGRLLLWFTWPAWPLVLWTLWRWRRQLLAPRQQRHLGLPLLIASVPLLSAVF
                     TLAGDRALLLALPALAALAALALPTFSRGAAALIDWFTVLFFSGWAIVIWVVWLAMQT
                     GVPAKPAANVARLAPGFEAPFQWPALVAALAGTLAWVALARWRTGRHRTALWKSLVLP
                     AAGATLCWLLLMTLWLPALDYGRSFAPQMREVRRWVGDAPCVQVHGLGTPQLVGVGFH
                     GGWPTVAAQGPARCPWLLVDVDAQASLPAAVHLHEWQLLTRIRRPTDDNESLLVFRRV
                     DGL"
     gene            8125..9156
                     /locus_tag="PK242_02900"
     CDS             8125..9156
                     /locus_tag="PK242_02900"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HOZ93000.1"
                     /translation="MPDLAYPDPTHWPGRAAPSAAPADDRWAVIDLGSNSFRLEIVRL
                     TDGQFERLDYLKETVRQGGGLGPDGRLSAQALQAGWDCLARFGQALQGFAPWRVRAIA
                     TQTLREARNGDEFLARGQRLLGFPIEVISGQEEARLIYRGVVQRLPASHERRLVLDVG
                     GRSTELVVGDGPQPRVMTSRLLGSVAWSMAHFGDQRLSAAQFERAEAAARTVLADAVA
                     SFGRPHWDAAYGCSGTVGAVADALRLGGLVTRRHRVTRAGLLQLRAQLIAAGDSRTLQ
                     LPGIKPERRAVIGGGVSVLCALFALLDIDRLRYVPGALRHGALHDWLERPQAGQARSA
                     APAARSARR"
     gene            complement(9222..9971)
                     /locus_tag="PK242_02905"
     CDS             complement(9222..9971)
                     /locus_tag="PK242_02905"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOZ93001.1"
                     /translation="MSLAPERSLGFAGASNFRDLGGYAGAGGLPVRWRRLFRSDHLAG
                     LTPQDAQQFQRLGVTRVFDFRGEQERAAVPYDLPGVTQHALTIEPTVVQRMKDLLDAG
                     EAITPERTVALMQQTYRGFVHDNADRFAELFAHLLADDAPLVFHCTAGKDRTGFAAAL
                     ILLALDVPRPVVMHDYLLTNAFYRMPAAHDSRAPREVLQVLWRVQEDFLNAALDIVEQ
                     EDGGVRGYLSRQLRVGEAERQRLQALYLAGA"
     gene            complement(10314..11330)
                     /locus_tag="PK242_02910"
     CDS             complement(10314..11330)
                     /locus_tag="PK242_02910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOZ93002.1"
                     /translation="MSLRPLGPFTVAAIGLGCMNICHAYGEPPPREQAVALLHAALDA
                     GVTHFDSAALYGFGASETLLGEVLGPRRQHITLASKCGMTVEAGDGSVRPGRVIDGRP
                     EALRATCEGSLRRLRTDVIDLYYLHRWDKQVPIEDSVGALADLVRAGKIRAIGLSEVS
                     AATLRRAHAVHPIAALQSEYSLWTRNPEIAALAACRELGVSFVAFSPVARGFLAGALP
                     DQAAVDALPDKDLRRPMPRFAPEHYARNLRLLHGFRAIAAELGCTPAQLALAWLLHQG
                     EDILPIPGTRSIAHLHENLGASGVRLSPEVLNRLEALINPATVSGHRYAEQARREIDT
                     EDSV"
     gene            complement(11386..12345)
                     /locus_tag="PK242_02915"
     CDS             complement(11386..12345)
                     /locus_tag="PK242_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOZ93003.1"
                     /translation="MSKTINVALAGAGAFGIKHLDGIRNIDGVRVVSLVGRELDKTKE
                     VAAKYGISHVTTDLADSLALPELDAVILCTPTQMHAAQAMQCLQAGKHVQVEIPLCDK
                     LADGQQVAALQKQTGLVAMCGHTRRFNPSHQYVHQKIAAGEFHIQQMDVQTYFFRRTN
                     MNALGQPRSWTDHLLWHHAAHTVDLFAYQAGSPIVQANAIQGPIHKELGIAMDMSIQL
                     KAANGAICTLSLSFNNDGPLGTFFRYIGDSATYIARYDDLVNGKEEKIDVSQVAVSMN
                     GIELQDREFFAAIREGREPNASVAQVLPCYEVLHRLEQQLNAT"
     gene            complement(12401..13261)
                     /locus_tag="PK242_02920"
     CDS             complement(12401..13261)
                     /locus_tag="PK242_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HOZ93004.1"
                     /translation="MGARITASVYSSHVPAIGAAIDHGKTAEPYWAPMFQGFEFSKRW
                     MKEHLPDVIFLVYNDHATNFDLSVIPTFAIGTAAEFEPADEGYGPRPVPRVKGHPRLA
                     SHIAQSVIQQDFDLTIVNQLKVDHGLTVPMNLMFGTPAEWPCAVIPFHVNVVQYPVPS
                     GARCFALGQAIRRAIESYDEPLKVQIWGTGGMSHQLQGPRAGLINAEWDNRFLDRLIA
                     DPKGLSAMPHIDYVREAGSEGIELVMWLIARGAMADVAGGPAPKVAHRFFHVPASNTA
                     VGYLILENQA"
     gene            complement(13397..13765)
                     /gene="ligA"
                     /locus_tag="PK242_02925"
                     /pseudo
     CDS             complement(13397..13765)
                     /gene="ligA"
                     /locus_tag="PK242_02925"
                     /inference="COORDINATES: protein motif:HMM:NF009917.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
     gene            complement(13919..14848)
                     /locus_tag="PK242_02930"
     CDS             complement(13919..14848)
                     /locus_tag="PK242_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOZ93005.1"
                     /translation="MSKPTSGDFTKTAGWLDWYDGPSTPRFRLPAGSVDAHCHVFGPG
                     AEFPYAPERKYTPCDAGKAQLFALRDHLGFARNVIVQATCHGADNRALVDALQASGGR
                     ARGVATVRRSVTDDELQALHAAGVRGVRFNFVKRLVDFTPKDELMEIAGRIARLGWHV
                     VIYFEAVDLPELWDFFTALPTTVVVDHMGRPDVGKPVDGPEFALFCKFMREHANVWSK
                     VSCPERLSVSGPPALSGEQAAYRDVVPFARHIVESFPDRVLWGTDWPHPNLKNHMPDD
                     GLLVDFIPHIAPTPELQRKLLVDNPMRLYWPEA"
     gene            complement(14859..15542)
                     /gene="ligK"
                     /locus_tag="PK242_02935"
     CDS             complement(14859..15542)
                     /gene="ligK"
                     /locus_tag="PK242_02935"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HOZ93006.1"
                     /translation="MYELGVVYRNIPRADGATADALAQFGSATVHEAMGRVGLLKPYM
                     RPIYPGAQVSGTAVTVLLQPGDNWMLHVAAEQIRPGDIVVAAVTAECTDGYFGDLLAT
                     SFQARGARALIIDAGVRDVRTLQQMGFPVWSKAISAKGCIKATLGSVNIPVVCAGMLV
                     TPGDVIVADDDGVVCVPAARAVLTLEAARKREANEGDKREIFASGTLGLDYYKMREGL
                     AKAGLRYID"
     gene            complement(15546..16574)
                     /locus_tag="PK242_02940"
     CDS             complement(15546..16574)
                     /locus_tag="PK242_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOZ93007.1"
                     /translation="MVIDCHGHYTTAPKALEQWRNRQIAGIQDPAAMPRVDELRISDD
                     ELRESIENNQLRLMRERGSDLTIFSPRASFMAHHIGDFQVSSTWAAICNELCHRVSQL
                     FPEHFIGAAMLPQSPGVDPASCIPELERCVRDYGFVGINLNPDPSGGHWTSPPLTDRH
                     WYPIYEKMVEWDIPAMIHVSTSCNACFHTTGAHYINADTTAVMQLIQGDLFKDFPTLR
                     FIIPHGGGAAPYHWGRYRGLAQELKKPLLQEHLLNNLFFDTCVYHQPGIDLLTKVIPV
                     DNILFASEMIGAVRGIDPETGHYFDDTRRYVQATANLNDEQRYKVYEGNARRVFPRLD
                     AALKARGR"
     gene            16717..17625
                     /locus_tag="PK242_02945"
     CDS             16717..17625
                     /locus_tag="PK242_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOZ93008.1"
                     /translation="MDLRQLEYFVRVAELGSFSRAALALDVAQPALSRQVRLLEVELR
                     QNLLTRHGRGAMPTEAGQVLLEHARGILHQIEHARDELARLRGGLAGRVTLGLPPSVA
                     RALAVPLVQAFGGQLPEAQLSITEALSASMQEQLASGRLDIAVLYNAQPVPGLDIAPL
                     ARDELLLVQHAGARADAAPATLRDLAQLPLVIPSRPNAIRMHVETQMAAAGCTPQVAL
                     EIDGVATILALVEGGAGSAILSRHALAGTAHPEAFALRRIATPDGQPLQIPLYTALSA
                     LRPASATRQAALTLLRAQALTHLGSF"
     gene            complement(17712..18158)
                     /locus_tag="PK242_02950"
     CDS             complement(17712..18158)
                     /locus_tag="PK242_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93009.1"
                     /translation="MPLRSVGLVAALLLAGHAQAAHGYQGRVHGSFAGHAIDVPVVCE
                     RPRLAGQRGNWFYAQSDPPTHEYAQDRNGDGVAVTVSFSGQEAMFMLYLAGQDHNFGN
                     TRREQIVLTEGGFVLTMSASRYEGKGRLRRKVGEDEIRLAVDCPQR"
     gene            18343..19281
                     /gene="rpoH"
                     /locus_tag="PK242_02955"
     CDS             18343..19281
                     /gene="rpoH"
                     /locus_tag="PK242_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HOZ93010.1"
                     /translation="MTQKVSSPSSNAVALTNPWSVVPPLGNLDAYISAVNRLPMLTLE
                     EEQRYARELRDTGSLEAAGRLVLSHLRLVVATSRQYLGYGLPHADLIQEGNVGLMKAV
                     KRFDPDQGVRLVSYALHWIKAEIHEYILKNWRLVKVATTKAQRKLFFNLRSMKQGFKA
                     ELDADLAHRDALTEEQIDVVARELNVKPDEVREMETRLAGGDVLLDPAPADDGEQAFG
                     PIAYLSDERHEPTAMLESAQRDVLATEGIATALRSLDERSRRIVTERWLNVNDDGSGG
                     MTLHELAAEYGVSAERIRQIEVAAMKKMRTALADYA"
     gene            19589..20584
                     /locus_tag="PK242_02960"
     CDS             19589..20584
                     /locus_tag="PK242_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ93011.1"
                     /translation="MHPTFTRRRLLAGAAVCAGLLAPLTTSHAQTAAAWPGKPLRFIV
                     GFPAGSSPDLTARALAEPLEKALGQPVVVENRVGAGGNIGGDAVAKATDGHTIGLMIN
                     GNLTIARLLNPALRYDPATDFAPISLIGVAPLVLVAPASAPGSDARSFLDAARAAGSK
                     WSYGSPGVGTVGHLGMELLKARTGIAPVHVPYPGYPQVFNALVGGDLQLSMLPPALAQ
                     TQIKAGKLRGIGVTGSGRSPLAPELPSLLDAGVKQFNLEIWNAVVAPAGMPRAHVARL
                     AEAISTIVRAPEMRQRLFQQGWQAVGSSPEGLANRIQADTRVLGQIIRTQGIRVD"
     gene            complement(20675..21286)
                     /locus_tag="PK242_02965"
     CDS             complement(20675..21286)
                     /locus_tag="PK242_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOZ93012.1"
                     /translation="MDRRLAIKNLAAGALLVSAWALLTACQPDRPNFHGIDITGVDYA
                     RDLRVRDFNGQPRTLADFRGKVVVIFFGYTQCPDVCPTTMGEMLRVKQLLGADGDKLQ
                     VLFLSLDPERDTPEVLKAYMGSFDPGFLGLYAGSADELAALAKDFKVFYKKVEGSTPG
                     SYTMDHTASSYIYDPQGRLRLVSRYGTRPEDVAADVRQLLGGA"
     gene            complement(21289..22203)
                     /gene="cyoE"
                     /locus_tag="PK242_02970"
     CDS             complement(21289..22203)
                     /gene="cyoE"
                     /locus_tag="PK242_02970"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOZ93013.1"
                     /translation="MSAPGTAAVLPSMPIWKQYYVLTKPRVVQLIVFCALIGMVLAVP
                     GLPDAAALQRMLIACVGIWLVAAAAAAFNCLVEKAIDARMRRTSWRPTARGELSAAQA
                     LGFSALLCALGSAVLYLWVNPLTMWLTFATFVGYAIIYTVILKPLTPQNIVIGGASGA
                     MPPVLGWAAMANAVEPQALILFLIIFLWTPPHFWALALYRVEDYRKSGLPMLPVTHGN
                     EFTRLQVLLYTLILLAACLLPFIYRMSGWLYLVAALVLNAGFIGYAFQLWRRYSDALA
                     RKTFRFSLIHLSLLFAALLVDHYLPWTR"
     gene            complement(22200..23336)
                     /locus_tag="PK242_02975"
     CDS             complement(22200..23336)
                     /locus_tag="PK242_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOZ93014.1"
                     /translation="MMDATPLYNLSPALRLMGMGLVVALGPLAWLWLRHQGAGPARRL
                     QALTLLTLFLTFDLVMFGAFTRLTDSGLGCPDWPGCYGQASPVGARVQIDAAEAAMPT
                     GPVTHGKAWVEMIHRYLATGVGVLILVLTLATWRQWRRQRAAAATDIVHPGWPLLTLL
                     WVCAQGAFGALTVTMKLFPAIVTLHLLGGVGLLVLLVVQAESYRRSAGAAPEPLAAGL
                     RALLWLSAGAVALQIALGGWVSTNYAVLVCNTFPMCQGSWWPPMDFAQGFELWRPLGE
                     SADGSRLEFHALTAIHYTHRLFAYAVFLLLGLLAWRLARAGRLAAQRRWVLGLAALQL
                     LTGLSNVILDWPLLAAVLHTGGAAALMTVLTWALCASHRPELSR"
     gene            complement(23333..23983)
                     /locus_tag="PK242_02980"
     CDS             complement(23333..23983)
                     /locus_tag="PK242_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93015.1"
                     /translation="MTVHNLPSPEALLAQQARRTRAGRWQMLLLALVCAAPVIASYYS
                     YYVVRPEARRSFGELIEPQRPLPVASATDLSGRPAALSALKGQWLLISVGGGACDAVC
                     ENHLYLQRQLREGLGKDKDRLDWVWLVSDDARLPEPLLPALGQATVLRVPPDVLGGWL
                     APAPGRALPDHLYVVDPMGHWMMRFPAGLDKQSAGRAKRDLERLMRASSSWDRAGR"
     gene            complement(24012..>24407)
                     /locus_tag="PK242_02985"
     CDS             complement(24012..>24407)
                     /locus_tag="PK242_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HOZ93016.1"
                     /translation="VLVQRGWAPRHRSERETLPPVQTPDGEVQVHGRVAEHPPRLYQL
                     GQEGLGAIRQNLDLDAYRAETGLPLAGLMVWQTGAPSEGLRRDWPEAASGVDKHYGYA
                     FQWFGLCALIGLLFVWFQVVRPIYRTRRA"
BASE COUNT         3699 a         8498 c         8569 g         3641 t
ORIGIN      
        1 cgcctgccat tgcagcgggt tggtgtcctg aaactcgtcg atcagcaggt ggcggatgcg
       61 cgcatccagc cgctgctgca cccaggccga cagctcgtga ttggccagca gttgcaaggc
      121 ggcgcgctcg atgtcgggta tgtcgatcca gccgcgtgcg cgcttgagct cggcaaagca
      181 gtcgatcagc gcgcgcgtca ggcgcaccat gcgctggtgg tgctggcggg cattgtgctg
      241 ctgctcggca gccagcagcg cgagcagttc ctcttcggcc tgctgcgcgg ccgggtatgc
      301 cttcaggtgc tgggtcaggc ggtcctcgtc ggccacaaaa aacgccttgc gcagccgggc
      361 caggcgcgcc gcgccatcac tctcaagcaa aaaggcctgc accaccgcgt cggccgcgtt
      421 ccggggcgtc ttgtttttct ccacccccag cgcgctggac caggcatgcc agcgcgcatg
      481 ggccgcgtcg gtcagcaggt tgtcggtcgg atcgtcaaaa cccgcaaact gcggatatag
      541 gctggcggcg ctggccaccg cctcatccac agcccccgcc gcatcggcca gcgcaaattc
      601 cacgcgccgc gccagcgcgc cttccagcgc ctgatgcgcg ttgtggcggc ccagctcggc
      661 caccagcgcc aggtagtcgg cacgcagggc ggcgtcgcca tcgaccgccc gcagaaaggg
      721 ccgccagacc tcggccacgg cgtcttcgtc gtcttccagc agctcgtagc gcaggggcag
      781 gcccaggtca tccagcagcg ccagcggcac gctttgcagc agcgcggcaa accagctgtg
      841 aaaggtgcgg atctgcaccg ggcggccctg ggcgagtagg ttttgataca aatttgatag
      901 ctgtttgcgc tgatctggca agcgttgggg ctcgattcct cttgcaatca gttcggcgtc
      961 taggcgcgcc tgccactgcg cgggcgactc gtctgcacgc ggccgagcaa agtcggccag
     1021 ccactgctgc aggcgctcgc gcatctcgcc tgccgccttc ttggtgaagg tgatggccag
     1081 aatttcgtgc gacgcgctgc ccgccagcag cgcgcgcagg atgcgcgaga ccagcaccca
     1141 ggtcttgccc gcgccggcgc aggcctccac cgccacgctg cggcgcggat cgcaggccac
     1201 cgcataaaac tgctcgcgcg tgcagggctg gccgttgtgt tcgtaggcgg gtgcgttcat
     1261 cttggcctga cggtacaagt tgcagacggg atccggcatg cagcgtgacc agaggccgcg
     1321 gagcaggcca tgccagcagc agccgtggcc ggggtttccc tggccactgg ctgcgccccc
     1381 tcgagggggg aggcgcaaca gcgcgcagcg cgtgaagcca cgggggtggg cgtcatcccc
     1441 agaaatcctt gcgacacagg ccgcgcacct cgcaccagtc gcacacgctg ccctcgccca
     1501 acgcgggcag gggcgcgccg gcggcgatct gcgtcatgtc gcgctctatg ccttcgtaca
     1561 gctgggcggc gcgctcggcc agatcgggca gttcgtgcag cttgggctcg ccgcgttcgg
     1621 gcaggttgag gtaggcggcg cgcggcgcgt ccaggcccga cagcagggca tagaagggca
     1681 gctgcgtgtc ttcgctgccc gctttgacac gggcgctggt tttttcaccc cgctcggtct
     1741 tgtagtcgat cagcagcggc gccccgtggg cgtcgcggtc gatgcggtcg atgcgcccgc
     1801 gcagctgtag cgtgccgcag gggccgctgc gctccacctc ggcggcggca aaggcaaagc
     1861 cgtcggcctc gtggtcggcc agccagttca gataggcatc gcgcagcgcc ggccaggcgg
     1921 cggcaaaggg catgaattcg ctgggctcga aggagccgtc cagcgtctgc gccgtcacct
     1981 gctgggcggc ctgctcgatc agcgccacgc ggtcggcgcc ggggctggcg gccagcgcgg
     2041 tgtgaaaggc ggccagcacc gcgtgcagcc agatccccca gtcgcgcttg tccacgtcca
     2101 ggtccagctc gtcatcgccc tgcaacccca gctggcgcag cgcgaagaag cggtacgggc
     2161 attcgcgcag ctgctgatag cggctggcgc tgagcggctg cgtgggcagc ggcgcgccgc
     2221 tgggccgcgg gcgttcggtg ggcgcggcgg ccacgctgcg gggcgtgcgc ggatcgggcg
     2281 catcgaccac gccggccgaa gcgcctggat tcagcgcgaa gtccagcgcc agcaccagcg
     2341 gcgaggccag cagcgactgg ccgctgtcgt cgccgtggcg ccacagcacg tcgagctgtg
     2401 gcgcgcccag cgccagcacc caggccgcgg cctgttcgcg ctgcagatcg gcgcgggtgg
     2461 gcaggtgcag ggcggcgcgc tgcgcgtcgc tccaggggcc gggcggctcg ggcgcggcgg
     2521 gcaggcgctg ctcgtcggcg ccgggcagca ccacggcggc aaacgggcgt gccagcagct
     2581 gcgccagcgg cagcaccacc acggcgggcg cgccaatctg tggggcacgg tagctggcgg
     2641 cttccagcgc ctcgctgacc cagcgcgtga actcgcccag ccccatgcgg cgctgcgccg
     2701 gtggccagtc gcgccaggcc gagccgtgcg cgtcgtccag cgccagcgcc tcgatcaggg
     2761 cgctgccagc ggcgtcctgc gccagcagcg gccacaggcc gcagccctgc agcagcgcgc
     2821 gcacatcggc cagccagcgc gtcagcgggc gcggcgcggc cagcggcgcg cgcagcgcct
     2881 cgatctgcgt ggtgagcggc tggccctggg tgatctgcgc ggcctgcgcc cagccgcgca
     2941 cggcgcgctg gcgcaaggcg tgctccagcg cggcgacggc gccgccgtca aaggccggcg
     3001 ccagcttgag ccagtccagc acggcatcgg tgctggcggc cggcgcgcag gcgcgcagcg
     3061 cggccatcag cgcggcgccg gcgtgggtgg tggacaggcg ccagccggtt tcgtcgtgca
     3121 gcgccgcgcc cgcctgcacg ccgcgcgcgg ccagcagggc gttgatgcgg cgcgtcaggc
     3181 cgcggtcggt ggccaccagc gcaaccggcg tgcggccggc gtgcaggtgg cgcagcacgc
     3241 aggcggcggc gcgctcggcc tcgtcttcgg cgtccaggca gcggtgcagc gcaacggccg
     3301 cgcccggcgc gggcgcgtca aacgccagca gcacggcttt ttcgctcagg tgctcggcca
     3361 ggtgggtggt cagcgcatcg gtctgcaggc ccggcacgat caccagcgcg tccagcgcgg
     3421 cggtggtgcg cggggcaaac aacacgtcgc tggcgtagtc cgaatgcgcg gcccaggcga
     3481 tggcgatgtg cgccagccgg ctctccagcg ccagcggccc gctggcggcc gccggcaacg
     3541 cggcgcgggc gcgctcggcc cagtcggggc gcagcgccgg cggcaggctg gcggccagcc
     3601 ggcccagcag gctggcctgc tccagcagcg ggccggccag ggcggtgcgc tggctggcca
     3661 ggccggcgcc ttccagcaag gtggcggcgg tgagcaaatc gcgcgcgggg tcgaacacca
     3721 gatcgtgcgg gccgggcacg aacaggccgg cacgctcggc ccagctgcgc gtggtctcga
     3781 agcgcggcgc gaaactgtcc ggaaactgcg ccgcccacag gcgcgcggcc agcggcagca
     3841 gctgggcata gggcagcagc accacgctgc gcgccgggtg cgccgccagc tggtgcaatt
     3901 gctggcgcac ctcggccagc agcgcgcgcc agggcgccgg cgcggcagcg gcgcccaggg
     3961 gttcatcgcc aacagctatg gcattcatag cgatcggcga tgccgcgcgg caaacggggg
     4021 catggggctt tctgtcacaa tccgggctac tttatccgct tggatcaccc cgtttctcaa
     4081 ggatgcccga tggccagtga cctgatcaaa cacgtgagcg acagctcctt cgaagccgac
     4141 gtcctgaagt ccgacaagcc ggtgctggtg gacttctggg ccgaatggtg cggcccctgc
     4201 cgcgccatcg cccccacgct ggacgagctg gccggcgcct acgacggcaa gctgcagatc
     4261 gccaagatca acgtcgacga caaccgcgcc gtgccggcca agttcggcat ccgcggcatc
     4321 ccgacgctga tggtgttcaa gaacggccag ctggcggcca ccaaggtcgg cgcgctgacc
     4381 aaggcgcaac tgacgcagtt catcgaccag caactggcct gagcggccgt cggcagccgg
     4441 cggcgcccac gccccggccg acggcgcgat ttttcctgcc ataatccctc ctcgaaagcg
     4501 gacccacagg taagggttcg ccggtgcccc ggtcacgggg cgcccgttcc ggagcctcgt
     4561 ttctgctggc tccgacagcc tccccagctt cctcgccatt tttcagcccc ccttggtctg
     4621 gccgatccac attccaggat cgatgtccac cgcaagacgc cgattgccat gcacctgaac
     4681 gaactcaaag tactgcacgt gtccgaactg ctcaagcagg ccgaggcgct ggagatcgac
     4741 aacgccagcc gcatgcgcaa gcaggagctg atgttcgcca tcatcaaaaa gcgcgccaag
     4801 ggcggcgagc aggtgtttgc cgacggcgtg ctggagatcc tgcccgacgg cttcggcttc
     4861 ctgcgcagcc ccgacaccag ctacaccgcc agcaccgacg acatctacat cagccccagc
     4921 caggtgcgcc gcttcaacct gcacaccggc gacatgatcg agggcgaggt gcgcatcccc
     4981 aaggacggcg agcgctactt tgcgctgacc aagctcgaca tggtcaacgg cggcccggcc
     5041 gagcagaaca agcacaaggt gatgttcgag aacctcacgc cgctgttccc gcgcgagcag
     5101 atgaggctcg agcgcgagaa cttcaagggc gaggagaaca tcaccggccg catcatcgac
     5161 gtcatcgccc ccatcggcaa gggccagcgc gcgctgatcg tggcgccgcc caagagcggc
     5221 aagacggtga tgatgcagac catcgcgcac gccatcagcg ccaactaccc ggacagccac
     5281 atgatggtgc tgctggtcga cgaacgcccc gaggaagtga ccgacatgca gcgctcggtc
     5341 aagggcgagg tgattgcctc caccttcgac gagccggcgg cgcgccacgt gcatgtggcc
     5401 gagatggtga tcgagcgcgc caagcgcctg gtcgagctca agaaagacgt ggtgatcctg
     5461 ctcgactcca tcacccgcct ggcacgcgcc tacaacaacg tggtgccctc cagcggcaag
     5521 gtgctgaccg gcggcgtcga cgccaacgcc ctgcaccgcc ccaagcgctt tctgggcgcg
     5581 gcgcgcaacg tcgaggaagg cggcagcctg accatcattg ccaccgccct gatcgatacc
     5641 ggcagccgca tggacgaggt gatcttcgag gaattcaagg gcaccggcaa cagcgaaatc
     5701 cacctggacc gccgcctgta cgaaaagcgc gtgttcccca gcatccagct caaccgctcg
     5761 ggcacgcggc gcgaggagct gctgctggcg cccgaggtgc tgcaaaagac ccgcatcctg
     5821 cgccagttca tgtacaacat ggacgagatc gaggcgatgg aaatggtcat caagagcatg
     5881 aaggcgacca agaccaacgt cgagttcttc gacatgatgc ggcgcggcgg ctgaacccct
     5941 ataattgccg gtttcgcgga aagtacgccc gatgtccggg cgcggctgcc gtaaccacct
     6001 caaggaattc atcatgcgtg aaggcattca ccccaactac cgcgaagtgt gcttcgtgga
     6061 cctgtccaac ggcttcaagt tcgtcacccg ctcctgcgtc aacaccaagg aaacggtgaa
     6121 gatggacgac ggtcgcgagc tgccgctgtt caagctcgac acctccagcg aatcgcaccc
     6181 cttctacacc ggcacccaga agtcggtcga caacatgggc ggtcgcgtcg agaagttccg
     6241 caaccgctac ggcaaggtcg cccgctgacg tcacacggcg ccgcactctg gccagccagg
     6301 gcagcacggg caaccgcgct gccctttttt tgtttggccg gccgccgccc tgcccgccct
     6361 tgaaccgtcc cagccccgcc atcgtcaccc aggacgcggt ccggcgcctg ccgcgctggg
     6421 cgctgctgct gctgtgcgtg gcctatgtga ttcccggcta cgtgggccgc gcgccgtgga
     6481 agaacgccga tctggcctcg ttcggctaca tgctggcact gcgcgacgcc ggcgacgggc
     6541 aggcgtggct gcaccccacg ctgatgggcg tggcgcccga cagcccggcc ctgctgccct
     6601 actggctggg cgcctgggcg atgcgctggc tgcagccgct cggcctggcc cccgacgtcg
     6661 ccgtgcgcct gccgtttgtg ctgctgctgg ccggcaccct ggtggccagt tggtcggcca
     6721 tgtacctgct ggcacgccac cccagcgcgc agccggtggc ctttgccttc ggtggcgaag
     6781 ccgagccgcg cgactacgcg cgtgcgctgg ccgacggcag cctgctggcg ctgatcgcca
     6841 ccctggggct ggcgcagttt tcgcacgaaa ccacgccggt gctggcgcag ctgttctttg
     6901 gcaccctggt gttctacggg ctggcggcgc tggcgacccg cccccggagc gccggtctgg
     6961 cgctggcctg cggcctgccc gggctgacgc tgtcgggcgc ccccaccacc gccctgctgt
     7021 acgccggact gagcgcgctg gcgctggcgc tgcccggccc cacccatgtg ccggcggcgg
     7081 tgcggcgcgg cgccctgtgg ctgctgccgg cgctgatggc cgcctgcctg gcgctggcgc
     7141 tcaccctggg actgtggccc accgagctgc cggcctggcg cagccgctgg agcgactggc
     7201 gctcgctcgg ccgcctgttg ctgtggttca cctggccggc ctggccgctg gtgctgtgga
     7261 ccctgtggcg ctggcgccgc cagttgctgg caccgcgcca gcagcgccac ctggggctgc
     7321 cgctgctgat cgccagcgtg ccgctgctga gtgcggtgtt caccctggcc ggcgaccggg
     7381 cgctgttgct ggcgctgccg gcgctggcgg cgctggcggc gctggcgctg cccaccttca
     7441 gccgcggtgc ggcagcgctg atcgactggt tcacggtgct gttcttcagc ggttgggcga
     7501 tcgtgatctg ggtggtgtgg ctggccatgc agaccggcgt gccggccaag ccggcggcca
     7561 acgtggcgcg gctggcgcca ggcttcgaag cgcccttcca gtggccggcc ctggtcgccg
     7621 cgttggccgg cacgctggcc tgggtggcgc tggcgcgctg gcgcaccggt cggcatcgca
     7681 ccgcgctgtg gaagagcctg gtgctgccgg ccgccggcgc caccctgtgc tggctgctgc
     7741 tgatgacgct gtggctgccg gcgctggact acggccgcag cttcgcgccg caaatgcgcg
     7801 aagtgcgccg ctgggtgggc gacgccccct gcgtgcaggt gcatggactg ggcacgccgc
     7861 agctggtggg cgtgggattt catggcggct ggccgaccgt ggccgcgcag ggcccggcgc
     7921 gctgcccctg gctgctggtg gacgtggacg cccaggccag cctgccagcg gcagtgcacc
     7981 tgcacgagtg gcagctgctg actcgcatcc ggcgtcccac ggacgacaat gaaagcctgc
     8041 tggtgtttcg ccgtgtggat ggcctgtaag ctatcattta catagctgct ggcgctttct
     8101 gcaattgccc tggacgtcgt tttcatgcct gacctggctt accctgatcc gacccactgg
     8161 cccggccggg cggcgccaag cgccgcgccg gcggacgacc gctgggcggt gatcgatctg
     8221 ggctccaaca gcttccggct ggagatcgtg cgcctgaccg acgggcagtt cgagcgcctc
     8281 gactacctca aggaaaccgt gcgccagggt ggcgggctgg ggcccgacgg ccgcctgtcg
     8341 gcgcaggcgc tgcaggccgg ctgggactgc ctggcgcgtt ttggccaggc cctgcagggg
     8401 tttgcgccct ggcgggtgcg cgccatcgcc acccagaccc tgcgcgaggc ccgcaacggc
     8461 gacgaattcc tggcccgcgg ccagcggttg ctgggctttc ccatcgaagt catcagcggg
     8521 caggaagaag cccgcctgat ctaccgcggc gtggtgcagc gcctgccggc gtcgcacgag
     8581 cggcgcctgg tgctggacgt cggcgggcgc tcgaccgagc tggtggtcgg tgacgggccg
     8641 cagccgcgcg tcatgacttc ccgcctgctg ggcagcgtgg cctggtcgat ggcgcatttc
     8701 ggcgatcagc ggctgtcggc cgcacagttc gagcgcgccg aggccgccgc ccggacggtg
     8761 ctggccgatg ccgtggccag cttcggccgg ccgcactggg atgccgccta cggctgctcc
     8821 ggcaccgtgg gcgcggtggc cgacgccttg cgcctgggcg gcctggtgac ccggcggcac
     8881 cgggtcacgc gcgccggcct gctgcagttg cgggcgcagc tgatcgccgc cggcgacagt
     8941 cgcacgctgc agctgcccgg catcaagccc gagcgccggg ccgtgatcgg tggcggtgtg
     9001 tcggtgctgt gcgcgctgtt cgcgctgctg gacatcgacc ggctgcgcta cgtgcccggc
     9061 gccctgcgcc acggcgcgct gcacgactgg ctggagcgcc cgcaggcggg ccaggcacgc
     9121 agcgccgccc cggcggcccg cagcgcgcgc cgctgacgcc agccccgtcg ctcacgcgga
     9181 ttcgcgttca actgccgttt ccagaataaa gcaggttcgc atcaggcacc cgccaggtac
     9241 agcgcctgca gtcgctggcg ctcggcctca cccacgcgca actggcgcga caggtagccg
     9301 cgcacaccgc cgtcttcctg ctcgacgatg tcgagcgcgg cgttcagaaa gtcttcctgc
     9361 acgcgccaca gcacctgcag cacctcgcgc ggggcgcggc tgtcgtgcgc ggcgggcatg
     9421 cggtagaacg cattggtcag caggtagtcg tgcatcacca ccgggcgcgg cacgtccagc
     9481 gccagcagga tcagggcggc ggcaaagccg gtgcggtcct tgccggcggt gcagtgaaac
     9541 accagcggcg cgtcgtcggc cagcagatgg gcgaacagtt cggcaaagcg gtcggcgttg
     9601 tcgtgcacga agccgcggta ggtctgctgc atcagcgcca ccgtgcgctc gggggtgatg
     9661 gcctcgcccg cgtccagcag gtccttcatg cgctgcacca cggtgggttc gatggtcagc
     9721 gcgtgctgcg tcacgccggg caggtcgtag ggcacggcgg cgcgttcctg ctcaccgcgg
     9781 aagtcgaaca cgcgcgtgac gcccaggcgc tggaactgct gcgcatcctg cggcgtcagc
     9841 ccggccagat ggtcggagcg aaacaacctg cgccagcgca ccggcagacc gcccgcgccg
     9901 gcgtagccgc ccaggtcgcg aaagttgctg gcaccggcaa agccaagaga tcgttcgggc
     9961 gcgagggaca tgggcgaggc atccacagaa accagcctcg attgtcggtc acgggcggac
    10021 gccgcgcatg acacgggtgt gaccgaggcc tgaggcgacg cgctcagcct agactcagcc
    10081 tagaaacggc agttgaccgc ttgtcaacgt cgggcagacc gcatcgatgc ggggtctggc
    10141 ggctgcgatg gccttcacct cctgggtgag cacgctacgc acccacccgc accgcgctcg
    10201 gcgcgccatg cggcgttgct cctgcttgcg ggcaggagcc cgcggcggcg gcgcgtcttg
    10261 cctggcaccc cgatcacggc acgggcgggc gcgtccaacc gccgtttcta ggctcagacg
    10321 ctgtcttcgg tgtcgatctc gcgccgcgcc tgctcggcgt agcgatggcc gctgacggtg
    10381 gcggggttga tcagcgcctc gaggcggttg agcacctcgg gcgacaggcg cacaccgctg
    10441 gcgcccaggt tctcgtgcag gtgagcgatg ctgcgcgtgc cgggaatcgg caggatgtcc
    10501 tcgccctggt gcagcagcca ggccagcgcc agctgcgccg gggtgcagcc cagctcggcg
    10561 gcgatggcgc gaaagccgtg cagcagccgc aggttgcgcg cgtagtgctc gggcgcaaag
    10621 cgcggcatgg ggcggcgcag gtccttgtcg ggcagcgcgt cgacggccgc ctggtcgggc
    10681 agcgcgccag ccagaaagcc gcgcgccacc gggctgaagg ccacgaagct gacgcccagc
    10741 tcgcggcagg cggccagcgc ggcgatttcg gggttgcgcg tccacagcga gtattcggat
    10801 tgcagcgccg cgatggggtg cacggcgtgg gcgcgccgca gcgtggcggc cgacacttcc
    10861 gacaggccga tggcgcggat cttgccggcg cgcaccaggt cggccagcgc ccccacgctg
    10921 tcttcgatcg gcacctgctt gtcccagcgg tgcaggtagt agaggtcgat gacgtcggtg
    10981 cgcaggcgcc gcaacgagcc ttcgcaggtg gcgcgcagcg cctcgggccg gccgtcgatg
    11041 acgcggccgg gccgcacgct gccgtcgccg gcctccaccg tcatgccgca cttgctggcc
    11101 agggtgatgt gctggcgccg cgggcccagc acctcgccca gcagggtttc gctggcgccg
    11161 aagccgtaca gcgcggcgct gtcgaaatgc gtgacgccag catccagcgc ggcgtgcagc
    11221 agcgccacgg cctgctcgcg cggcggcggc tcgccgtagg cgtggcagat gttcatgcag
    11281 cccaggccga tggccgcgac ggtgaagggg ccgagagggc gcaacgacat gttcaaaatc
    11341 aaaatgggct gcagcgcacg tcaggcaagc gccggcagct atgcttcaag tagcgttgag
    11401 ctgctgctcc agccggtgca gcacctcgta gcagggcagc acctgggcca cgctggcgtt
    11461 gggctcgcgg ccctcgcgga tggcggcgaa gaactcgcgg tcctgcagct cgatgccgtt
    11521 catcgacacc gccacctggg acacgtcgat cttttcttcc ttgccattga ccaggtcgtc
    11581 gtagcgcgcg atgtaggtgg cgctgtcgcc aatgtagcga aagaaggtgc ccagcgggcc
    11641 gtcgttgttg aagctcaagg acagcgtgca gatcgcgcca ttggccgcct tgagctggat
    11701 actcatgtcc atggcgatgc ccagctcctt gtggatcggg ccctggatgg cgttggcctg
    11761 cacgatcggg ctgccggcct gatacgcgaa cagatcgacc gtgtgcgccg cgtggtgcca
    11821 cagcaggtgg tcggtccacg agcgcggctg gcccagggcg ttcatgttgg tgcggcgaaa
    11881 gaagtaggtc tgcacgtcca tctgctggat gtggaattcg cccgccgcga tcttctggtg
    11941 aacgtactgg tggctggggt tgaagcgccg ggtgtggcca cacatggcga ccaggccggt
    12001 ttgcttctgc agcgcggcca cctgttggcc gtcggccagc ttgtcgcaca gcgggatctc
    12061 gacctgcacg tgcttgccgg cctgcaggca ctgcatggcc tgggcggcgt gcatctgggt
    12121 gggggtgcac aggatgacgg cgtcgagctc cggcagggcc aggctgtcgg ccaggtcggt
    12181 ggtcacgtgg gaaatgccgt acttggcagc cacctccttc gtcttgtcca gctcgcgacc
    12241 gaccagcgag acgacccgca cgccgtcgat gttgcggatg ccgtccaggt gcttgatgcc
    12301 gaaggcgccg gcgccggcca gcgcaacgtt gatggtcttg ctcatgatgc tcctataaca
    12361 atagcttctg gcgcttgcca gacaagcgcc gcaagccgat tcaagcttga ttttccagga
    12421 tcaggtagcc caccgccgtg ttggacgccg gcacatggaa gaagcggtgc gccaccttgg
    12481 gcgcggggcc gcccgccacg tcggccatgg cgccgcgcgc gatcagccac atcaccagct
    12541 cgatgccctc gctgccggct tcgcgcacgt agtcgatgtg cggcatggcg gacaggccct
    12601 tcgggtcggc gatcaggcgg tccaggaagc ggttgtccca ctcggcgttg atcaggccgg
    12661 cgcgcgggcc ctgcaactgg tggctcatgc cgccggtgcc ccagatctgc accttcagcg
    12721 gctcgtcgta gctttcgatg gcgcggcgga tcgcttggcc cagcgcaaag cagcgcgcgc
    12781 ccgagggcac cgggtactgc accacgttga cgtggaacgg aatcaccgcg cagggccatt
    12841 cggcgggggt gccgaacatc aggttcatcg gcaccgtcag gccgtgatcg accttgagct
    12901 ggttgacgat ggtgaggtcg aagtcctgct ggatcaccga ctgcgcgatg tgcgaggcca
    12961 ggcgcgggtg gcccttgacc ctgggtaccg ggcgcgggcc gtagccttcg tcggcgggct
    13021 cgaattcggc cgcggtgccg atggcgaagg tggggatgac gctgaggtcg aagttggtgg
    13081 cgtggtcgtt gtagacgaga aagatgacgt cgggcaggtg ctccttcatc caccgcttgc
    13141 tgaactcgaa accctggaac atgggcgccc agtagggctc ggccgtcttg ccgtggtcga
    13201 tggcggcgcc gatcgccggc acatgcgagg aatacaccga ggcggtgatg cgggcgccca
    13261 tggttgctat tgaattggca gctgtctgcg cttgctggac ggacgctgcc agcccatttt
    13321 gtgccttggc ctgccaggcc ttccaggctt cggactggct catcgggccc tgggcgtcgc
    13381 cgccctcgcc cttgtagcgg ttgccctcgg ggtggcggcc gccgcgcacc atcatgtcgc
    13441 ggtactgctg ctcggtcatg ccggtcatgc tgcccgccat ctgctgaaac gacaggccgt
    13501 cggtggcgcc cagcttggac aggaagtaga tgttgccgcc ggtcgccatc atccagttca
    13561 ggtcgcgcgc gcgcaccgcc gccttctgct cgtcggtcat gggccactcg gccaggtagg
    13621 cgtcctcgtc ctgcttgaag cgggcgcggt tgtcggcctt catcaggctc atgcagaact
    13681 ggttgagcca gtagcccttg cggctctggt cggcatcgaa gatggtggtg ccgggaacgt
    13741 cgagataggg tttgttgaga gacatcgcca ttgctcctgc gcagtcagcg cgatcgatca
    13801 tcaatgaagc cggaggccgc ggagcaggcc ataccagcga acgccgtggc cggacctgcg
    13861 ccggccactg gcgttgtccc cctccggggg aaggcgcgaa gcgactcagg gggaatcatc
    13921 atgcctcggg ccagtacagg cgcatcgggt tgtccaccag cagcttgcgt tgcagctcgg
    13981 gcgtgggcgc gatgtgcggg atgaagtcca ccagcagccc atcgtcgggc atgtggttct
    14041 tgaggttggg gtgtggccag tcggtgcccc acagcacgcg gtccggaaag ctttcgacga
    14101 tgtggcgggc gaacggcacc acgtcgcggt aggcggcctg ctcgccgctg agcgccggcg
    14161 ggccgctgac cgacaggcgt tcggggcagc tcaccttgct ccacacgttg gcgtgctcgc
    14221 gcatgaactt gcaaaacagc gcgaactcgg ggccgtcgac cggtttgccg acgtcggggc
    14281 ggcccatgtg atcgaccacc acggtggtgg gcagggcggt gaagaagtcc cacagctcgg
    14341 gcagatcgac cgcctcgaag tagatcacca cgtgccagcc cagccgggcg atgcggccgg
    14401 cgatctccat cagttcgtcc ttgggcgtga agtcgaccag gcgcttgacg aagttgaagc
    14461 gcacgccgcg cacgccggcc gcgtgcaggg cctgcagttc gtcgtcggtg acgctgcggc
    14521 gcaccgtggc cacgccgcgc gcccggccgc cggaggcttg cagcgcatcc accagcgccc
    14581 ggttgtcggc gccgtggcag gtggcctgca cgatcacgtt gcgcgcaaag ccgagatggt
    14641 cgcgcagcgc gaacaactgg gccttgccgg cgtcgcaggg cgtgtacttg cgctcgggcg
    14701 cgtagggaaa ttcggcgccc gggccgaaca cgtggcagtg cgcgtccacg ctgccggccg
    14761 gcagcctgaa acgcggggtg ctggggccgt cgtaccaatc gagccaaccg gccgtcttgg
    14821 tgaagtcgcc gctggtgggt ttgctcatcg cgcggccctc aatcgatgta gcgcagcccg
    14881 gccttggcca gcccttcgcg catcttgtag tagtccagcc ccagcgtgcc gctggcgaag
    14941 atctcgcgct tgtcgccttc gttggcctcg cgcttgcgcg ccgcttccag cgtcagcacc
    15001 gcgcgcgcgg cgggcacgca gaccacgccg tcgtcgtcgg ccacgatcac atcgcccggc
    15061 gtcaccagca tgccggcgca cacgacgggg atgttgaccg agcccagcgt ggccttgatg
    15121 cagcccttgg cgctgatcgc cttgctccag accggaaagc ccatctgctg cagggtgcgc
    15181 acgtcgcgca cgccggcgtc gatgatgagg gcgcgcgcgc cgcgggcctg gaagctggtg
    15241 gccagcaggt cgccgaagta gccgtcggtg cattcggccg tgaccgccgc caccacgatg
    15301 tcgcccgggc ggatctgctc ggccgccacg tgcagcatcc agttgtcgcc cggctgcagc
    15361 agcacggtga cggccgtgcc gctgacctgc gcgccggggt agatgggccg catgtagggc
    15421 ttgagcaggc cgacgcggcc catggcctcg tgcacggtgg cgctgccgaa ctgcgccagc
    15481 gcgtcggccg tggcgccgtc ggcgcgcggg atgttgcgat acaccactcc gagttcatac
    15541 atggctcatc tccccctggc cttgagtgcc gcgtccagcc gcggaaacac gcgccgcgcg
    15601 ttgccttcgt agaccttgta gcgctgctcg tcgttcaggt tggcggtggc ctgcacgtag
    15661 cgccgggtgt cgtcgaagta gtgcccggtt tcaggatcga tgccgcgcac ggcaccgatc
    15721 atctcgctgg cgaacaggat gttgtcgacc ggaatcacct tggtcagcag gtcgatgccc
    15781 ggctggtgat agacgcaggt gtcgaagaac aggttgttca gcaggtgctc ctgcagcagg
    15841 ggctttttca gctcctgcgc caggccgcgg tagcgccccc agtggtaggg cgcggcgccg
    15901 ccaccgtgcg ggatgatgaa gcgcagcgtc gggaagtcct tgaacagatc gccctgaatg
    15961 agctgcatca cggcggtggt gtcggcgttg atgtagtgcg ccccggtggt gtgaaagcac
    16021 gcgttgcagg acgtgctgac gtggatcatg gccgggatgt cccactcgac cattttttcg
    16081 tagatcgggt accagtggcg gtcggtcagc ggcgggctgg tccagtgacc gccggaaggg
    16141 tcggggttca ggttgatgcc cacgaagccg tagtcgcgca cgcaccgctc cagctcggga
    16201 atgcagctgg ccggatcgac cccgggcgac tgcggcagca tggcggcacc aatgaaatgc
    16261 tcgggaaaca gctggctgac gcggtggcac aactcattgc agatcgccgc ccaggtgctg
    16321 gagacctgaa agtcgccgat gtgatgcgcc atgaagctgg cgcgcgggct gaagatggtc
    16381 aggtcgctgc cgcgctcgcg catcaggcgc agctggttgt tctcgatcga ttcgcgcagt
    16441 tcgtcgtcgc tgatccgcag ctcgtcgacg cgcggcatcg ctgccgggtc ctgaatgccg
    16501 gcgatctgcc ggttgcgcca ctgttcgagc gccttgggcg cggtggtgta gtggccgtgg
    16561 cagtcgatga ccagggggtg gtgcaagctc atggtgttcc tctgctgacg ctggcgcatt
    16621 gtgcgcagcg gcagggccgc tgaccagcca cccaccccac tagcagctag agcaaaatgg
    16681 catacctgaa tcacagccat gtcgtaaatt gggaccatgg acctgcgtca actcgaatac
    16741 ttcgtccgcg tggccgagct gggcagcttc agccgcgccg ccctggcgct ggacgtggcc
    16801 cagcccgcgc tcagccgcca ggtgcgcctg ctggaagtgg agctgcgtca gaacctgctg
    16861 acacgccacg gccgcggcgc catgcccacc gaagccggcc aggtgctgct ggagcacgcg
    16921 cgcggcatcc tgcaccagat cgaacacgcg cgcgacgagc tggcgcgcct gcgcggcggc
    16981 ctggccggtc gcgtgaccct gggcctgccg cccagcgtgg cgcgcgccct ggcggtaccg
    17041 ctggtgcagg ccttcggcgg gcagttgccc gaagcccagc tgtcgatcac cgaggcgctg
    17101 tcggccagca tgcaggagca actggccagc gggcggctgg acatcgccgt gctctataac
    17161 gcccagccgg tgcccgggct ggacatcgcg ccgctggcgc gcgacgagct gctgctggtg
    17221 cagcacgccg gcgcccgcgc cgacgccgct cctgccacgc tgcgcgacct tgcgcagctg
    17281 cccctggtca tccccagccg gcccaacgcc atccgcatgc acgtcgagac gcagatggcc
    17341 gcggccggct gcacgccgca ggtggcgctg gagatcgatg gcgtggccac catcctggcg
    17401 ctggtcgaag gcggcgccgg cagcgccatc ctgtcgcgcc acgccctggc cggcaccgcg
    17461 caccccgagg cctttgcgct gcgccgcatc gcgacgccgg acggccagcc gctgcagatc
    17521 ccgctctaca ccgccctcag cgccctgcgc ccggccagcg ccacccgcca ggcggcgctg
    17581 acgctgctgc gcgcccaggc gctgacgcat ctgggcagct tctgaaatac taccaaaact
    17641 atagctgctt gcgcagtatt ggtgcgcctt taagcccagt ttgatctgga acgccggcga
    17701 tccggcgggg ctcagcgctg agggcagtcg acggccaggc ggatctcgtc ttcgccgacc
    17761 ttgcggcgca gccggccctt gccttcgtag cgcgaggcgc tcatggtgag cacgaagccg
    17821 ccttcggtga gcacgatctg ctcacgccgg gtgttgccga agttgtggtc ctggcctgcc
    17881 aggtagagca tgaacatggc ctcctgaccg ctgaacgaga ccgtcaccgc cacgccgtcg
    17941 ccgttgcggt cctgggcgta ttcgtgcgtc ggcgggtcag actgcgcgta gaaccagttg
    18001 ccccgctgcc ccgccagcct ggggcgctcg cagaccaccg gcacatcgat ggcatgcccg
    18061 gcaaagctgc cgtgcacgcg gccctggtag ccgtgggcgg cctgtgcgtg gccggccagc
    18121 agcagcgcgg ccaccaggcc gacagatcgc aagggcatgg tcggggtcct cttttgtaag
    18181 gcgcaggcga tgcgcgcgag tgtagcgggc gcagccgcgc gcccggcccg gcgcgcccgc
    18241 cggcccggtc agccatggtt cgggcgcggg cggaactttg cccttagcac tccctccaat
    18301 cgagtgctaa aatacttgag ccacacgaaa ggacattcaa cgatgaccca gaaggtatcc
    18361 agccccagca gcaacgccgt cgcactgacc aacccctggt cggtggtgcc tccgttgggc
    18421 aatctggacg cctacatcag cgcggtcaat cgcctgccga tgctgacgct ggaggaagag
    18481 cagcgctacg cccgcgagct gcgcgacacc ggcagcctgg aagccgccgg tcggctggtg
    18541 ctgtcgcacc tgcggctggt ggtggccacc tcgcgccagt acctgggcta tggcctgccg
    18601 cacgccgacc tgatccagga aggcaacgtc ggcctgatga aggccgtcaa gcgcttcgac
    18661 cccgaccagg gcgtgcgcct ggtgagctac gcgctgcact ggatcaaggc cgagatccac
    18721 gaatacatcc tgaagaactg gcgcctggtg aaggtcgcca ccaccaaggc gcagcgcaag
    18781 ctgttcttca acctgcgctc catgaagcag ggtttcaagg ccgaactcga cgccgatctg
    18841 gcgcaccgcg acgccctgac cgaggagcag atcgacgtcg tggcgcgcga gctgaacgtc
    18901 aagcccgacg aggtgcgcga aatggaaacc cgcctggccg gcggcgacgt gctgctggat
    18961 ccggcgccgg ccgacgacgg cgaacaggcc ttcggcccca tcgcctacct gtcggacgag
    19021 cgccacgagc cgaccgccat gctcgaatcg gcccagcgcg acgtgctggc caccgaaggc
    19081 atcgccaccg ccttgcgcag cctggacgag cgcagccgcc gcatcgtcac cgagcgctgg
    19141 ctgaacgtca acgacgacgg ctcgggcggc atgacgctgc atgagctggc ggccgaatac
    19201 ggcgtcagcg ccgagcgcat ccgccagatc gaggtggcgg ccatgaagaa aatgcgcacc
    19261 gcactggccg actacgcctg agccgcgcgc ctgcgcggcc tgcccctgcg ccccgcccac
    19321 agccccgtcg gcggggtttt tcatgccgcc agcgggggct cgggcacgcc ggccctgcgc
    19381 gaagagctat agtctttgaa tctgacaacg gccgtggccc gcgggtgcgc ttggcgagag
    19441 gcgcatccga accggtctcg cggcgtcgca ccctgcctgg cacgccgagc gcggcgcggg
    19501 cgggcacgtc caacggtcgt tttcaggttg ccgttgccgg ttccccggcg cccgtccctc
    19561 tctgaccttt gccctgcacc gcccgaccat gcaccccacg ttcacccgcc gccgtctgct
    19621 cgcgggcgcc gccgtctgcg ccggcctgct cgcccccctg accaccagcc acgcacagac
    19681 tgccgcagcc tggcccggca aaccgctgcg cttcatcgtc ggctttccgg ccggctcctc
    19741 gcccgacctg acggcgcgcg cactggccga gccgctggaa aaagccctgg gccagccggt
    19801 ggtggtggaa aaccgtgtcg gcgccggcgg caacatcggc ggcgacgccg tggccaaggc
    19861 caccgacggt cacaccatcg gcctgatgat caatggcaac ctgaccatcg ccaggctgct
    19921 caacccggcc ctgcgctacg acccggcgac cgacttcgcc cccatcagcc tgatcggcgt
    19981 ggcgcccctg gtgctggtgg cgccggccag cgcgcccggc agcgacgccc gcagcttcct
    20041 ggacgccgcg cgcgccgccg gctcgaagtg gagctacggc tcgcccgggg tcggcaccgt
    20101 cggccacctg ggcatggagc tgctgaaggc gcgcaccggc atcgccccgg tgcatgtgcc
    20161 ctaccccggc tacccgcagg tgttcaacgc cctggtcggc ggcgacctgc aactgtccat
    20221 gctgccaccg gcgctggcgc agacgcagat caaggccggc aagctgcgcg gcatcggggt
    20281 caccggcagc ggccgcagcc cgctggcgcc cgaactgccc agcctgctgg acgccggcgt
    20341 gaagcagttc aacctggaaa tctggaacgc cgtggtggcg ccggccggca tgccgcgcgc
    20401 ccatgtggcc cggctggccg aggccatcag caccatcgtg cgcgcaccgg aaatgcgcca
    20461 gcgcctgttt cagcagggct ggcaggcggt gggctcgtcg cccgaagggc tggccaaccg
    20521 catccaggcc gacacccgcg tgctgggcca gatcatccgc acgcagggca ttcgggtcga
    20581 ctgagggtca ccctgccacg atcatgaaac gatagcgcct ggcgcacgcc ctgcaagctc
    20641 tggcgctggt tttgaccgcc ctgcgggcag gccgtcaggc gccgccgagc agctggcgca
    20701 cgtcggccgc cacgtcctcg ggccgggtac cgtagcggct gaccaggcgc agccggccct
    20761 gcgggtcgta gatgtagctg gaggccgtgt ggtccatggt gtagctgccc ggcgtgctgc
    20821 cctcgacctt cttgtagaac accttgaagt ccttggccag cgccgccagc tcgtcggcgg
    20881 agccggcgta caggcccagg aagccggggt cgaagctgcc catgtaggcc ttgagcacct
    20941 cgggcgtgtc gcgctcgggg tccaggctga ggaacagcac ctgcagcttg tcgccatcgg
    21001 cgcccagcag ttgcttgacg cgcagcatct cgcccatggt ggtggggcag acgtcgggac
    21061 actgggtgta gccgaagaag atcaccacca ccttgccgcg gaagtcggcc agcgtgcgcg
    21121 gctggccatt gaagtcgcgc acgcgcaggt cccgggcgta gtcgaccccg gtgatgtcga
    21181 ttccgtgaaa gttcggcctg tccggctggc aagcggtcaa caaggcccag gcgcttacca
    21241 gcaaagcgcc agcagctaga tttttaatag caagacgacg atccatgttc aacgggtcca
    21301 gggcaggtag tgatcgacca gcagcgccgc aaacagcagc gacaggtgga tcagcgagaa
    21361 gcggaaggtc ttgcgcgcca gcgcgtcgga gtagcggcgc cagagctgga aggcatagcc
    21421 gatgaagccg gcgttcaaca ccagcgccgc caccagatac agccagccgc tcatgcggta
    21481 gatgaagggc agcaggcagg ccgccagcag gatgagggtg tagagcagca cctgcaggcg
    21541 cgtgaactcg ttgccgtgcg tcaccggcag catcggcagg ccgctcttgc ggtagtcctc
    21601 cacccggtac agcgccagcg cccagaagtg cggcggcgtc cacaagaaga tgatcaggaa
    21661 caggatcagc gcctgcggct ccaccgcgtt ggccatggcg gcccagccca gcaccggcgg
    21721 catggcgccc gaggcgccgc cgatgacgat gttctgcggc gtcagcggct tgaggatgac
    21781 ggtgtagatg atggcgtagc cgacgaaggt ggcaaacgtc agccacatgg tcagcgggtt
    21841 gacccacagg tacagcacgg ccgagccgag cgcgcacagc agtgccgaaa accccagcgc
    21901 ctgcgcggcc gacagctcgc cgcgcgcggt ggggcgccag ctggtgcgcc gcatgcgcgc
    21961 gtcgatggcc ttttcgacca ggcagttgaa ggccgccgcc gccgccgcca ccagccagat
    22021 gccgacgcag gcgatcagca tgcgctgcag cgccgctgcg tccggcaggc ccggcaccgc
    22081 cagcaccatg ccgatcaggg cgcagaacac gatcagctgc accacgcgcg gcttggtcag
    22141 cacgtagtac tgcttccaga tcggcatgga cggcagcacg gcggcggtac cgggggcgct
    22201 catcgggaca gctccggccg gtggctggcg cacagggccc aggtcagcac cgtcatcagc
    22261 gccgcggcgc cgccggtgtg cagcacggcg gccagcagcg gccagtccag aatcacgttg
    22321 gacaggccgg tcagcaactg cagcgccgcc agccccagca cccagcgccg ctgcgccgcc
    22381 agccggccgg cgcgcgccag ccgccaggcc agcaggccca gcagcaggaa cacggcgtag
    22441 gcgaacagcc ggtgcgtgta gtggatggcg gtgagcgcat ggaactccag ccgcgagccg
    22501 tccgccgact cgcccagcgg ccgccacagc tcgaagccct gggcaaagtc catcggcggc
    22561 caccagctgc cctggcacat cgggaaggtg ttgcacacca gcacggcgta gttggtgctg
    22621 acccagccgc ccagcgcgat ctgcagcgcc accgcaccgg ccgacagcca cagcagcgca
    22681 cgcaggccgg cagccagcgg ctcgggcgcg gcaccggcgc tgcggcggta gctttcggcc
    22741 tgcaccacca gcagcaccag caggcccacg ccgcccagca ggtgcagggt gacgatggcc
    22801 ggaaacagct tcatggtgac cgtcagcgcg ccaaaggcac cctgggcgca gacccacagc
    22861 aacgtcagca gcggccagcc cgggtgcacg atgtcggtcg cggcggcggc gcgctggcgt
    22921 cgccactggc gccaggtggc cagggtcagc accaggatca gcacgcccac cccggtggcc
    22981 aggtagcggt ggatcatctc gacccaggcc ttgccgtgcg tcaccgggcc ggtcggcatg
    23041 gcggcctcgg cggcgtcgat ctgcacacgc gcgccgaccg ggctggcctg accgtagcag
    23101 ccgggccagt cggggcagcc cagaccggaa tcggtcaggc gcgtgaaggc gccgaacatc
    23161 accaggtcga aggtcagaaa cagcgtcagc agggtcagcg cctgcaggcg ccgcgccggg
    23221 ccagcgccct ggtggcgcag ccacagccag gccagcgggc ccagcgccac caccaggccc
    23281 atgcccatca ggcgcaacgc ggggctgagg ttgtacagcg gcgtggcgtc catcatcggc
    23341 ccgcccgatc ccagctggag gaggcgcgca tcagccgctc cagatcgcgt ttggcgcggc
    23401 cggcgctctg cttgtccagc ccggcgggga aacgcatcat ccagtggccc atggggtcga
    23461 ccacgtacag gtggtcgggc agcgcccgcc ccggcgccgg cgccagccag ccgcccagca
    23521 cgtcgggcgg cacacgcagc accgtggcct ggcccagcgc cggcaacagc ggctcgggca
    23581 ggcgcgcgtc gtcgctgacc agccagaccc agtccagccg gtccttgtcc ttgcccaggc
    23641 cttcgcgcag ctggcgctgc aggtacaggt ggttttcaca caccgcatcg cacgcgccgc
    23701 cgcccacgct gatcagcaac cactggccct tgagggcaga cagtgccgcc gggcgcccgc
    23761 tcaggtcggt ggcgctggcg accggcagcg gccgctgcgg ctcgatcagc tcgccaaaac
    23821 tgcgccgcgc ctcgggccgc accacgtagt agctgtagta ggaggcgatc accggcgccg
    23881 cgcacaccag cgccagcagc agcatctgcc agcgaccggc gcgcgtgcgc cgtgcctgct
    23941 gcgccagcag agcttcgggc gaaggcaggt tgtgcacggt catgccgaac gcctggttgg
    24001 cccggccgct ctcaggcgcg tcgggtacgg tagataggtc tgacaacttg aaaccacaca
    24061 aaaagcaggc cgatgagggc gcacaggccg aaccactgga acgcatagcc gtagtgcttg
    24121 tcgacaccac tggcggcctc gggccagtcg cggcgcaggc cctcggatgg cgcgccagtc
    24181 tgccacacca tcaggccggc cagcggcagg ccggtttcgg cacggtaggc gtcgaggtcc
    24241 agattctgcc ggatcgcccc gagcccctcc tgccccagtt gataaagcct cggcggatgt
    24301 tcggccacgc gaccatgcac ctgcacctcg ccgtccggcg tctgcaccgg cggcagcgtc
    24361 tcgcgctcgc tgcggtggcg cggcgcccag ccgcgctgca ccagcac
//
LOCUS       DAOOOG010000019        23491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_2064_length_23491_cov_28.235706, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000019 DAOOOG010000000
VERSION     DAOOOG010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 23491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23491
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2064_length_23491_cov_28.235706"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PK242_02990"
     CDS             <1..604
                     /locus_tag="PK242_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93017.1"
                     /translation="RRFARTGSELELDLPDTIRSTAANAGWLDIRMVPERHNAVKVLL
                     LMDVGGTMDEHIQRVEELFSAVKAEMKHLEFYYFHNCVYDFMWKNNRRRFAEKFPTWD
                     IIRKYNKDYKLIFVGDATMSPYEILQPGGSVEYNNEEPGAEWLQRLTHAFPKFVWINP
                     EPQGVWQYRQSIAIIQQLMGQRMFPLTLKGLEDAMRMLSK"
     gene            complement(617..2107)
                     /locus_tag="PK242_02995"
     CDS             complement(617..2107)
                     /locus_tag="PK242_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOZ93018.1"
                     /translation="MIDEARFAQRACAPAPARRASPATWIGRCLMLSLALLTGCSTVR
                     PWINTPMAQEQAQVSHVNRTRDPSLVMAVTISGGGARAAAFGYGVLRALDEVRFDLNG
                     KHLTLLEATDLISGVSGGSIVSAYYAAFGREGLSNFEPEFLRKNFQQGLISLLTHPGS
                     LHELSSPWFGRSNLLQRQLDVLYRGMTFADMEARPRHPQLIVTATDLTRGNGFEFTWD
                     QFALICSDLGQVPLSFAVAASSAVPIVLSPLTVKNYANQCPADVSASVAHSAGVGASG
                     VADYRQRLYRAQINEYLDASKRPYIHLVDGGLADNLGVQRLLDRAVMSGGLRQTFREV
                     DIPPGSVRKVVLVVVNAERDPTRNLDLSDRVPGVFDVADALLFGTGARATHETQEFLN
                     DVVRAWHKEVDRRGRSPETDVFAPDAEIHVITVNLRDVSVDRRPRLLQVETAFSIPDA
                     EVTELIAAGRDVLRASPAFQRLTQSLNARIEAADAVPAGPAAERVD"
     gene            2348..4258
                     /locus_tag="PK242_03000"
     CDS             2348..4258
                     /locus_tag="PK242_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOZ93019.1"
                     /translation="MTRPFVDFARRRGWLAAALLACLLALPGCALLPSTDPARADASG
                     ERPAPAPQRSDTARDAPTASAFDLRVECDDAELRTLVERHAELLRYRAITDLDEAELA
                     RLLVLAERDVRQLLATEGYFSPVVKLRRDGTRGARPTVVIAIEPGAPTTVARVDIGFE
                     GQIASDDDADAVAQRRAIVDGWSLDQGRRFTQQRWSDAKTDALRALVARRYPRGRIAY
                     SLADVDAAGGRAHLHLRLDSGPLFRLGPAQVQGAQRYPPELAERLSWLRPGQVYDQKQ
                     LVDAQQRLTGSGYYDSAYLSIDPEGDAAAVPVSFTVTEARRHKVQLGVGYSTDAGPRL
                     TLEHRDNRAFASSWRSQTRLNLNRKTPLLQAELTSLPDAGGWRWGALARYQRQDDGLL
                     NTLSRTLRIGRSRIEATYDRNVYLNYEHASVTGSATRTVPDALLGDGAALSAGYAWTG
                     RHFDRLPLPTRGWGLGLDASAGITTVGARRPFARLTAHWLGIVPLAGGASRLALRAEG
                     GAVIADRRARLPGSHLFRTGGDTTVRGYGWRRIGMPVGQGLVGPGRYLALGSVEWQRP
                     ILQDRWPGLLEHSLFIDVGSVANRADRLRPHWGVGTGLRMITPVGPMQLDIAYGLKSK
                     EIRLHMTVGLVF"
     gene            4369..8292
                     /locus_tag="PK242_03005"
     CDS             4369..8292
                     /locus_tag="PK242_03005"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOZ93020.1"
                     /translation="MRWPQRLLAGLLAGLLAAAGLAAGGLWLWAGSEGSLATALAWVG
                     RHQPLQAEGVRGTLRGGGTLQRLVWSQDGLQLQAEQAEWRWHPGALLWRRLHIERLSA
                     HRLRIDDQRPADRPTARPPEALTLPLQVRVAELRVDELHWRGPPALRLDDIAGHYAYD
                     GGQHRLTLERARFEQGRYRLHAAATAHAPIRLDLALTGALSATVPGGAAALPLTLQAT
                     LRGPLTELQAQADLQAAAPGTAPEPPALPPLLGFAARAPQQQPRAPTPPAAAAQASPQ
                     ARLSARLTPWAAQPVAEAHARVHALDLAALWSQAPHTRLNGALDLMPLPGTAQTGWAL
                     VADLTNPAAGPWDQGRLPVQALQADALWQDDTARVRTLKARAGGGTLELDGHWRRATE
                     AAPPWQIDARLSGVDPAQLHGQLAAFPIDGTARLRGAGHATDFELALQARTSPAPAAA
                     PPARDTLAHGLRALRLQSAHAQGRWDAGLLRLERLQLRSDDAELSGHAQWRLQGSDAP
                     GGRAELRLSAPGLRASAQGELHAQRGGGQLSAELSDAARALAWARRLPGSQRWLGDAQ
                     AQGRATLQARWRGGWRDPTLDARLSVPKLDWRGAGSPPLQLRELQLTADGRLAQARLD
                     LRGQIAQGERRLRLALRASGGRSTPDTPLAQASWRVQVEPWQAEWHDPALGAGAWQLA
                     GQGVLPLRWSPARGGQFEAGAGTLTITSPAPASQARLSWEPLRWQAGELDSRGRLSGL
                     PQQWLERLAGTPLQDAGLSGDLVFDGAWQATLGRQLRLDARLARARGDLTLQLTDADT
                     GVRSRLAAGLRQAQLSLRSDGPALTLDLLWDSERAGRVEGQLRTALVAGSDADGRTRW
                     SWPPDAALAGSLRAQLPQIAAWSPLAPPGWRLGGALAAELQLAGTRAAPSVTGSLSAD
                     GVALRSVVDGLQFKDGRLRARLDGARLRIDEFSLAGVGASGSGGRLIASGEAAWRDGR
                     PQAQLNATLQRLRVSARDDRQLSVSGALQAALDGRALRTTGRLTVDQALIVLPDDSRP
                     TLGDDVIVRGPGGQVRAGRQADGRAPPPSGAPDAPARTPLNAHVDVQIDLGPEFRLRG
                     MGIDTRLAGLLTLTADGPLTGLPHVRGTVRTVGGTFHAYSQQLRIERGQLLFQGAADN
                     PALDILALRPQLGHDQRVGVQVAGTALLPRVRLYSAPALPDNQTLAWLLLGRPAPDTG
                     AEAAMLQSAALALLGGREGRGLAARFGLDELSFSGTGDGELNQASVTLGKRLSERLYA
                     AYEHSIAGTGGTLMIFYELSRRWSLRGQAGAENSALDLIFKLSFD"
     gene            complement(8323..8757)
                     /locus_tag="PK242_03010"
     CDS             complement(8323..8757)
                     /locus_tag="PK242_03010"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOZ93021.1"
                     /translation="MKIELPEHKQLVFEMTIPIRWGDMDAMGHINNAVYFRYMETARI
                     DWLHGIGCQPDPGGEGVVIVNAFCNFIRQFTYPGDILIKTFVSHLGRSSFDTWATMER
                     TDQPGVVCATGGATVVWVNFPAQKSAPLPDWLRQRLSPAPGG"
     gene            complement(8851..9633)
                     /locus_tag="PK242_03015"
     CDS             complement(8851..9633)
                     /locus_tag="PK242_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ93022.1"
                     /translation="MSYSIDLSGRVALVTGASGGLGAQFARTLARAGAGVVLASRRLE
                     KLKDLRARIEGDGGDAHVVECDVTDVHSIRAAVAHAETEMGSIDILVNNSGVSTTQRI
                     QDVSEQDYDFVFDTNVRGAFFVAQEVGKRMLARARGAAPGSFTGGRIINIASMAGLRA
                     LPQIGVYCMSKAAVIQMTRAMALEWGRHGINVNAICPGYIDTEINHHHWQTEKGRKLI
                     DMLPRKRVGEPADLDAVLMMLASDQSHFVNGAVIAADDGFAL"
     gene            9795..11495
                     /locus_tag="PK242_03020"
     CDS             9795..11495
                     /locus_tag="PK242_03020"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HOZ93023.1"
                     /translation="MTPEQIIQQYGPREAMEYDVVIVGAGPSGLSAAIRIKQRAAEQG
                     QEVSVVVLEKGSEPGAHILSGAVMDPKALTELIPDWKAKGAPLNQPVTDDAYVFLSET
                     GGTRVPGVLLPPFAHNHGNYIVSLGNVVRWLAEQAEALGVEIFPGFAAAEVLFNDDGS
                     VKGVATGNMGVGKDGEPTENFQLGMELHAKYTIFAEGARGHLGKQLIARFKLDAQSDP
                     QSYAIGIKELWEIDPSRHTPGFVMHTAGWPMDEHTYGGAFLYHAEDHKVYCGYVVGLN
                     YRNPYLSPFEEFQRWKTHPRIKWYFTDDRGQVNAKRLSYGARAITAGGVLALPRTIFP
                     GGALVGCDAGYLNVSRIKGSHAAIKTGSLAGEAACDAVLAGRQHDELTAYPEAFDHSW
                     LAAELNKDRNFKNWFKKGLTVGTVMNGFEQYVLRGHAPWTLHRAEPDHVQLKAAAECA
                     PIDYPKPDGKLTFDRLSSVFISNTNHEENQPAHLTLKDPGVPVNVNLAKFAGPEGRYC
                     PAGVYEFVPDEARGGNAQRLQINAQNCVHCKTCDIKDPTQNIVWVTPEGGGGPNYSGM
                     "
     gene            complement(11542..12342)
                     /locus_tag="PK242_03025"
     CDS             complement(11542..12342)
                     /locus_tag="PK242_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HOZ93024.1"
                     /translation="MPRFAANLSMLYNELDFLDRFAAAASDGFTAVEYLFPYAWAPEQ
                     LSALLRQHGLRQVLFNAPPGEWDAGERGLACIPGREGEFHAGLERAIAYARALDCPRI
                     HVMAGLMPAGVDAATLRATYVANLRGAAEQAAPHGIDILIEPINPRDMPGFYLQRQDQ
                     AHALLDAIDRPNVKVQMDLYHCQIVEGDLAMKIRHHLPGGRVGHIQIAGVPQRHEPDL
                     GEIHHPYLFELLDRLGYEGWIGCEYRPARGAVPGGTSDGLGWLRPYLA"
     gene            complement(12353..13015)
                     /locus_tag="PK242_03030"
     CDS             complement(12353..13015)
                     /locus_tag="PK242_03030"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HOZ93025.1"
                     /translation="MSEARAREEIVRVGRSLFERGYVHATAGNISVRLADGYLITPTD
                     ACLGTLDPARLARLDAQGRQTGGDPASKTLALHRRIYEASAGTAAPAGCVVHSHSTHL
                     VACSLRADPAAAELLPPLTPYFVMKVGRVPHIPYHRPGAPEAAEAVARAIARHAEAGH
                     PIRAVMLARLGPNVWHDTPAAAMATLEELEETARLWLLAPAAPLNTAQIDELRRAFNA
                     FW"
     gene            complement(13027..14310)
                     /locus_tag="PK242_03035"
     CDS             complement(13027..14310)
                     /locus_tag="PK242_03035"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOZ93026.1"
                     /translation="MTPPILLGCIADDFTGATDLANNLVRAGMRVVQTIGVPEAPLAA
                     DAAADAVVVALKSRTIAADQAVAQSLAALRWLRAQGARQIYFKYCSTFDSTPAGNIGP
                     VTEALMQALDCDFTIATPAFPDNGRTVFKGYLFVGDQLLSESGMRQHPLTPMTDANLV
                     RVLQAQTRRRVGLIEQRTLAAGSDAIRARIADLRAEGVGIAIVDAVSNDDLWRLGPAL
                     AELPLVTAGSGVAIALPANFGLAPAAQAAALPAASGLRAVLAGSCSQATQAQVAHFIA
                     CGHPALALDPLRMAAGDAVVRQALDWAQGRLAADAPLLVYSTAEPAAVQAIQQRLGVA
                     RAGELVEQTLAAIARGLVGLGVRQLVVAGGETSGACVQALGVAQLRIGAQIDPGVPWC
                     HAASPLAPQGLHIALKSGNFGGEDFFTRAFAVLRP"
     gene            complement(14307..15215)
                     /locus_tag="PK242_03040"
     CDS             complement(14307..15215)
                     /locus_tag="PK242_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOZ93027.1"
                     /translation="MSDRHVGVIGLGAMGLGIACTLRQRGYRVRACDARAGVAAAFAA
                     EGGVACTTPAEVANGVRVVVSVVVNAAQTEQVLFGSDGAAAAMAPGSVFVMCSTVDPN
                     WSMALEARLAQHGILYVDGPISGGAARAASGQMTMMTAATPAAYAAADAVLEAMAGKV
                     YRLGQRAGAGSQVKIINQLLAGVHIAAAAEAMALGLREGVDPAALYEVITHSAGNSWM
                     FENRMAHVLAGDYTPLSAVDIFVKDLGLVLDTARASKFPLPLAATAHQMFMQASSAGH
                     ALEDDSAVIKIFPGIELPAPTDKERP"
     gene            15380..16408
                     /locus_tag="PK242_03045"
     CDS             15380..16408
                     /locus_tag="PK242_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOZ93028.1"
                     /translation="MHIVITGGAGFLGVRLARALLQRDALSLAGAAPRPLTRLTLVDR
                     VAPPAALTQDPRVRVVTGELDALLTPDASGQTRAVPTDSDAVVHLAAAVSGECEADFD
                     LGLRSNLDATRALLQTCRALGTRPLFVFSSSLAVFGHTPEQPMPAIIEDDTLPTPQNS
                     YGTQKYMSELLVADCTRKGFVRGRNVRLMTVSVRPGRPNAAASSFLSGIVREPLAGQR
                     AVCPVAPDTRVALSSPARTIEGLVRALEASDEDWGPRTALNLPALSTTAGDMVAALAR
                     VAGPEVAARVDWVADAGIARIVGAWPAGMNARRAARLGLRADASFDDIIRDHLRENPV
                     PPAAAARP"
     gene            16480..17451
                     /locus_tag="PK242_03050"
     CDS             16480..17451
                     /locus_tag="PK242_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOZ93029.1"
                     /translation="MKLHQLALGLGLALGLVAGAQAQTTMKISISVAQNSHQGIAIDT
                     FAKEVEKRTGGRYKIQTFYSGALGGERESIEAVQLGTQELAFSSTGPVPNFVPEVKIL
                     DVPFLFRDKAHARAVLDGPIGQDMLAKFEPKGFKALAWAENGFRHMTNSKRDVKTPED
                     LKGLKMRTMENPVHVAAYKGFGIITTPMAFPEVFTALQQGTVDGQENPLSVIISAKFD
                     QVQKHLTLTGHVYSPAIFLMNKGAFDKLSAADKQAFIEAAKEGTKANRARVDEDDAKG
                     VADLRAKGMTVIDTIDKAKFVSALAPVNAQFEKDFGKANLDRIRNFK"
     gene            17556..18050
                     /locus_tag="PK242_03055"
     CDS             17556..18050
                     /locus_tag="PK242_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOZ93030.1"
                     /translation="MKAFILTLEGWTTRIAMVLACLMLALAAALGVFQIFTRFVLEQP
                     AEWSEVLIRFSLIWMVFLGIPMAFRQGAMVSVDALYRAVPTGAKRVLDLVVALAALAL
                     MLIVLWWGWDYAVRGSVQTMAGLESLSMFWAYLAMPVGAAFSLLGILANYLDPRRMEL
                     ETAQ"
     gene            18121..19395
                     /locus_tag="PK242_03060"
     CDS             18121..19395
                     /locus_tag="PK242_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOZ93031.1"
                     /translation="MTSAMLITMVLGFALSVSVAVSIGLAAIVGIQASQAHMLISAKE
                     MFNAINKFPLAAIPFFILAGNLMETGGISRRLVEFAKSLVGGVQGGLPMTCVLTCMIF
                     AAVSGSSVATTFAIGAILIPALIKHGYPTTWAAALQATSAELGVIIPPSIPMILFGVS
                     AEVSIGELFIAGFGPGLLISGALMLFVWLYCKWQGWGRNDGDGRLGVVAATRQAAWAL
                     LMPVIILGGIYGGIFTPTEASAVAVLYALVVGMLIYREIGVKDLYVTLRKSVVSSAVI
                     MFIIANAGLFAFLITRAGVPEAIGHWLEQVLQNPALFLLGVNAALFVIGMFIETSAAI
                     IVLAPILVPVAQRFGIDPVHFGLVMVVNLALGMITPPFGVNLFAACTVARVSLDKIIR
                     HLVPFVGVVLACLMVITYVPTLSLALRDLVYR"
     regulatory      19421..19501
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      19502..19593
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            19653..20783
                     /gene="gcvT"
                     /locus_tag="PK242_03065"
     CDS             19653..20783
                     /gene="gcvT"
                     /locus_tag="PK242_03065"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOZ93032.1"
                     /translation="MSAADSPLLKTPLHALHLELGARMVPFAGYEMPVQYQAGLIAEH
                     RHTREAAGLFDVSHMGQLKLIGPDAAAALETLMPVDVQGLAENKQRYGLLLTDEGTII
                     DDLMFVNRGDHLFLIVNGACKAGDIAHMQARIGSRCLVQPLPDQALLALQGPQAVGAL
                     QRLVAGVEKLVFMTGGAFDWLGTPLYVTRSGYTGEDGFEISLPGERAEAFARALLAQP
                     EVKPIGLGARNSLRLEAGLCLYGNDIDHTTSPVEAGLNWAMQKVRRSGGARAGGFPGA
                     ARILAQLDGTQPVERQRIALMARERVPVREPAELRNLDGQQVGQVTSGLLSPTLDKPI
                     AMGYVRPGFATIGTELFAMVRGKPVPMEVVATPFVPNRYHRG"
     gene            20849..21217
                     /gene="gcvH"
                     /locus_tag="PK242_03070"
     CDS             20849..21217
                     /gene="gcvH"
                     /locus_tag="PK242_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOZ93033.1"
                     /translation="MSLKYTEDHEWLKIEGDVATVGITHHAQDALGDVVFVELPEVGR
                     HYAQKDAAGVVESVKAAADVYMPVSGEVLEVNEALREDPSLANSDPQGAGWFFRIRLS
                     EPAQAGALMDEAAYTAFSAG"
     gene            21367..>23491
                     /gene="gcvP"
                     /locus_tag="PK242_03075"
     CDS             21367..>23491
                     /gene="gcvP"
                     /locus_tag="PK242_03075"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOZ93034.1"
                     /translation="MPPMSQPTLPELENPGEFIARHIGPSPAEEAHMLSVIGEASRRA
                     LIDSILPPDIARRTPMDLPPACSEADALAELKAIASRNRHQMKSFIGQGYYGCHLPGV
                     ILRNILENPAWYTAYTPYQAEISQGRMEALVNFQTMVTDLTGMDIANASMLDEATAAA
                     EAMTLARRTVKAKGQVIVLSGDCHPQTIEVMRTRAAPLGLTLRLANSAAEWDAAIAAD
                     DYFAALIQYPASSGWLMDWSDEVEKIHAKGAAAIFAADLLALTLLKTPGEMGADIVVG
                     STQRFGMPMGAGGPHAAYMACRDDYKRSMPGRLVGVSVDAHGQPAYRLALQTREQHIR
                     REKATSNICTAQVLPAVIASMYAVYHGPVGLTRIAERTARLTAILKAGLAQLGFTHVH
                     HDSAFDTLCLKTEGATDSIAARAVQMGANLRKAWDSCLCVSLDETSTRADVELLWRIF
                     AGDARALPGFDALAATAPALIPAALRRTSGFLTHPVFNTHHSETAMLRYIRQLSDKDL
                     ALDRSMIPLGSCTMKLNATSEMIPITWPEFANVHPFAPADQRQGYAALDRQLRAWLSS
                     ITGYADVSLQPNAGSQGEYAGLLAIRAYHAAQGQGQRDICLIPASAHGTNPASAMMVG
                     LKVVVTQCDEQGNVDLADLQAQCEKHAAHLACVMITYPSTHGVFETQVKRLCEIVHQH
                     GGRVYLDGANLNAMVGLAAPGEFGGD"
BASE COUNT         3527 a         8578 c         8157 g         3229 t
ORIGIN      
        1 gcgccggttc gcccgcaccg gcagcgagct ggaactggac ctgcccgaca ccatccgctc
       61 caccgccgcc aacgccggct ggctggacat ccgcatggtg cccgagcgcc acaacgccgt
      121 gaaggtgctg ctgctgatgg acgtgggcgg caccatggac gagcacatcc agcgcgtgga
      181 agagctgttt tccgccgtca aggccgagat gaagcacctg gagttctatt acttccacaa
      241 ctgcgtctac gacttcatgt ggaagaacaa ccggcgccgc tttgccgaga agttcccgac
      301 ctgggacatc atccgcaagt acaacaagga ctacaagctg atcttcgtcg gcgacgcgac
      361 gatgagcccc tacgagatcc tgcagccggg cggcagcgtc gaatacaaca acgaggagcc
      421 cggcgccgaa tggctgcagc gcctcaccca cgccttcccg aagttcgtct ggatcaaccc
      481 cgagccgcag ggcgtgtggc agtaccgtca gagcatcgcc atcatccagc aactgatggg
      541 ccagcgcatg tttccgctga ccctgaaggg cctcgaggac gcgatgcgca tgctgagcaa
      601 ataaccgcac agcgcgtcag tcgacgcgct cggccgccgg gccggccggc acggcatctg
      661 cggcctcgat gcgggcgttc agcgactgcg tcagccgctg gaaggccggc gaggcccgca
      721 gcacgtcacg gccggcggcg atcagctcgg tgacctcggc atccgggatc gagaacgcgg
      781 tctccacctg cagcaggcgc gggcgccggt ccaccgacac gtcgcgcagg ttcacggtga
      841 tcacatggat ctcggcgtcg ggcgcaaaca cgtcggtctc gggcgagcgg ccgcggcggt
      901 cgacctcctt gtgccaggca cgcaccacgt cgttgaggaa ttcctgcgtc tcgtgggtcg
      961 cgcgcgcacc ggtgccaaac agcagggcgt cggccacgtc gaagacgccc ggcacccggt
     1021 cgctcaggtc gagattgcgc gtggggtcgc gctcggcgtt gaccaccacc agcaccacct
     1081 tgcgcacgct gccgggcggg atgtcgacct cgcgaaaggt ctggcgcagc ccgccgctca
     1141 tcaccgcgcg gtccagcagg cgctgcaccc ccaggttgtc ggccaggccg ccatccacca
     1201 ggtggatgta ggggcgcttg ctggcatcca ggtattcgtt gatctgcgcg cgataaaggc
     1261 gctggcggta gtcggccacg ccgctggcgc cgaccccggc gctgtgcgcc accgacgccg
     1321 acacgtcggc cgggcactgg ttggcatagt tcttcaccgt gagcgggctg agcacgatcg
     1381 gcaccgccga cgaggccgcc accgcaaacg acagcggcac ctgccccagg tccgagcaga
     1441 tcagcgcgaa ctgatcccag gtgaactcga agccgttgcc gcgcgtcagg tcggtcgcgg
     1501 tcacgatcag ctgcgggtgg cgcgggcgtg cctccatgtc ggcaaaggtc atgccgcgat
     1561 acagcacgtc gagctggcgc tgcaacaggt tggagcggcc gaaccagggc gagctgagct
     1621 cgtgcaggct gccggggtgg gtcagcagcg agatcaggcc ctgctggaag ttcttgcgca
     1681 ggaactcggg ctcgaaattg gacaggccct cgcgcccgaa ggccgcgtaa tacgccgaca
     1741 cgatggagcc gcccgacacg ccgctgatca ggtcggtcgc ctccagcagg gtcaggtgct
     1801 tgccattcag gtcgaagcgc acctcgtcca gggcgcgcag cacgccatag ccaaacgccg
     1861 ccgcgcgcgc cccgccaccc gaaatggtga ccgccatcac caggctgggg tcgcgcgtgc
     1921 ggttgacgtg cgagacctgc gcctgctcct gggccatcgg cgtgttgatc cacggtcgca
     1981 cggtcgaaca acccgtcagc agcgccaggc tcagcatcag gcagcgcccg atccaggtgg
     2041 ccgggctggc gcggcgtgcc ggggcaggcg cgcaggcgcg ctgggcgaaa cgtgcttcgt
     2101 cgatcatgac ttgattctat gggcagccga cgatcaccag ccgacgcccg agacccccga
     2161 tcgctgccgc gcgggagcgt gtggacgtct tgtttgctat cctattcgta gctgtcggcg
     2221 caacacccgt gcgatccatc ggcaatctgg ttttcgatgt gccactccac gccgccggcc
     2281 ccaggaccct gcgcggggcc agggtggcgc gccgcgtcgg cccggcacgc ccactaaact
     2341 cgcgccggtg acgcgccctt tcgttgactt tgcccgccgt cgcgggtggc tggcagccgc
     2401 cctgctggcc tgcctgctgg cgctgcccgg ctgcgccctg ctgccgagca ccgaccccgc
     2461 gcgcgcagac gccagcggcg agcgcccggc cccggcgccc cagcgcagcg acaccgcacg
     2521 cgacgcgccg accgccagcg ccttcgacct gcgcgtcgag tgcgacgacg ccgagctgcg
     2581 caccctggtg gaacgtcacg ccgaactgct gcgctaccgc gccatcaccg acctggacga
     2641 ggccgagctg gcgcgcctgc tggtgctggc cgaacgcgac gtgcgccagc tgctggccac
     2701 cgagggttac ttcagccccg tggtgaagct gcggcgcgac ggcacccgcg gcgcacggcc
     2761 gacggtggtg atcgccatcg agcccggcgc ccccaccacc gtggcgcgcg tggacatcgg
     2821 gttcgaaggc cagatcgcca gcgacgacga cgccgacgcg gtggcgcagc ggcgcgccat
     2881 cgtggacggc tggtcgctgg accagggccg ccgcttcacc cagcagcgct ggtcggacgc
     2941 caagaccgac gccctgcgcg ccctggtggc gcggcgctac ccgcgcggcc gcatcgccta
     3001 cagcctggcc gacgtggacg ccgccggcgg ccgcgcccac ctgcacctgc ggctggactc
     3061 ggggccgctg ttccggctcg ggccggcgca ggtacagggc gcgcagcgct acccgcccga
     3121 gctggccgag cgcctgtcct ggctgcgccc gggccaggtg tacgaccaga agcagctggt
     3181 ggacgcccag cagcgcctga ccggcagcgg ctactacgac tcggcctacc tcagcatcga
     3241 ccccgagggc gatgcggccg ccgtgccggt cagcttcacc gtcaccgaag ccaggcgcca
     3301 caaggtgcag ctgggtgtcg gctacagcac cgacgccggc ccgcgcctga cgctcgagca
     3361 ccgcgacaac cgcgcgttcg ccagttcgtg gcgcagccag acgcgcctga acctcaatcg
     3421 caagacgccg ctgctgcagg ccgaactgac ctcgctgccc gatgccggcg gctggcgctg
     3481 gggcgcgctg gcgcgctacc agcggcagga cgacggcctg ctcaacaccc tctcgcgcac
     3541 gctgcgcatc ggccgcagcc gcatcgaggc gacgtacgac cgcaacgtct acctgaacta
     3601 cgagcacgcc agcgtgaccg gctcggccac ccgcaccgtg cccgacgcgc tgctgggcga
     3661 cggcgccgca ctcagcgccg gctacgcctg gaccggccgc cacttcgacc gcctgccgct
     3721 gcccacccgc ggctggggcc tgggtctgga cgccagcgcc ggcatcacca ccgtcggcgc
     3781 gcgccgcccg ttcgcgcgcc tgaccgcgca ctggctgggc atcgtcccgc tggccggcgg
     3841 cgccagccgg ctggcgctgc gcgccgaagg cggcgcggtg atcgccgacc ggcgcgcgcg
     3901 cctgcccggc agccacctgt ttcgcaccgg tggcgacacc acggtgcgcg gctacggctg
     3961 gcggcgcatc ggcatgccgg tgggccaggg cctggtcggg ccgggccgct acctggcgct
     4021 gggcagcgtc gaatggcagc gccccatcct gcaggaccgc tggcccggcc tgctggagca
     4081 cagcctgttc atcgacgtcg gcagcgtggc caaccgcgcc gaccggctgc gcccgcactg
     4141 gggcgtgggc accggactgc gcatgatcac cccggtgggg ccgatgcagc tggacatcgc
     4201 ctacggcctc aagtcgaaag aaatccgcct gcacatgacc gtgggcctgg tcttctgatg
     4261 gctgcgcaga tccctccgga tgctcctgat tcaatagctg tcggcgcacg accagaaagc
     4321 gctgcaagcc aatttgattc acagccgccg gctcggcgcc gtggcctggt gcgctggccg
     4381 cagcgcctgc tggccggcct gctggccggc ctgctggcgg ctgccgggct ggccgccggc
     4441 gggctctggc tgtgggccgg cagcgaaggc tcgctggcca ccgcgctggc ctgggtcggc
     4501 aggcaccagc cactgcaggc cgaaggcgtg cgcggcacgc tgcgcggcgg cggcacgctg
     4561 cagcgcctgg tctggtcgca ggacggcctg cagctgcagg ccgagcaggc cgagtggcgc
     4621 tggcaccccg gcgccctgct gtggcgccgt ctgcacatcg agcggctgtc ggcgcaccgg
     4681 ctgcgcatcg acgaccagcg ccccgccgac cgaccgaccg cccgcccgcc cgaggcgctc
     4741 acgctgccac tgcaggtgcg cgtggccgag ctgcgggtgg acgagctgca ctggcgcggc
     4801 ccacccgccc tgcgtctgga cgacatcgcc ggccactacg cctatgacgg cggccagcac
     4861 cggctgacgc tggagcgcgc ccgcttcgag cagggccgct accggctgca cgccgccgcc
     4921 accgcccatg cgccgatccg gctcgacctg gcgctgaccg gcgcgctcag cgccaccgtg
     4981 ccgggcggcg ccgctgcgct gccgctgacg ctgcaggcca cgctgcgcgg cccgctgacc
     5041 gagctgcagg cgcaggccga cctgcaggcg gcggcgcccg gcaccgcacc cgagccgccc
     5101 gccctgccgc cgctgctcgg ctttgccgcg cgcgcgccgc agcagcagcc ccgggcgccg
     5161 acgccgcccg ccgcggcggc gcaggcgtcg ccccaggcgc gcctcagcgc ccgcctcacc
     5221 ccctgggccg cacagccggt ggccgaggcg catgcccgcg tgcacgcgct cgacctggcc
     5281 gcgctgtggt cccaggcgcc acacacgcgg ctgaacgggg cgctcgacct catgcccctg
     5341 cccggcaccg cccagaccgg ctgggccttg gtggccgacc tcaccaaccc cgccgccggc
     5401 ccctgggacc agggccgcct gccggtgcag gcgctgcagg ccgatgccct gtggcaggac
     5461 gacaccgccc gcgtgcgcac actcaaggcg cgcgccggcg gcggcaccct ggagctggac
     5521 ggccactggc gccgcgccac cgaagcggcg ccgccctggc agatcgacgc ccgcctgagc
     5581 ggcgtcgatc cggcccagct gcacgggcag ctggccgcct tcccgatcga tggcacggcg
     5641 cgcctgcgtg gcgccggcca cgccaccgac ttcgagctgg cgctgcaggc ccgcaccagc
     5701 cccgcgcccg ccgctgcgcc gcctgcccgc gacacgttgg cgcacgggct gcgcgcgctg
     5761 cgcctgcagt ccgcccacgc ccaggggcgc tgggacgccg gcctgctgcg gctggagcgc
     5821 ctgcagctgc gcagcgacga cgccgagctg agcggccacg cccagtggcg cctgcagggc
     5881 agcgacgcgc cgggcgggcg cgccgagctg cgcctgagcg cacccgggct gcgcgccagc
     5941 gcccagggcg agctgcatgc ccagcgcggc ggcggccagc tgagcgccga gctgagcgac
     6001 gccgcccgcg ccctggcctg ggcgcgccgc ctgccgggca gccagcgctg gctgggcgac
     6061 gcccaggccc agggccgcgc cacgctgcag gcgcgctggc gcggtggctg gcgcgacccg
     6121 acgctggacg cccgcctgag cgtgccgaag ctggactggc gcggcgccgg cagcccgccg
     6181 ctgcagctgc gcgagctgca gctgacggcc gacgggcggc tggcgcaggc gcggctcgac
     6241 ctgcgcggcc agatcgccca gggcgaacgg cgcctgcgcc tggccctgcg cgccagcggc
     6301 ggccgcagca cgccggacac gccgctggcc caggccagct ggcgcgtgca ggtggagcca
     6361 tggcaggccg agtggcacga tccggcgctg ggcgcaggcg cctggcagct ggccggccag
     6421 ggcgtgctgc cgctgcgctg gtcgccggcg cgcggcggcc agttcgaagc cggcgccggc
     6481 acgctcacca tcacctcacc ggccccggcg tcgcaggcgc ggctgagctg ggagccgctg
     6541 cgctggcagg ccggcgagct ggacagccgc gggcgcctga gcggcttgcc acagcagtgg
     6601 ctggaacgcc tggccggcac gccgctgcag gacgccggcc tgtccggcga cctggtgttc
     6661 gacggcgcgt ggcaggccac gctcgggcgc cagctgcggc tggacgcccg cctggcgcgc
     6721 gcgcgcggcg acctcacgct gcagctcacc gacgccgaca ccggcgtgcg ctcgcgcctg
     6781 gccgccggtc tgcgccaggc gcagctgagc ctgcgcagcg acgggccggc gctcaccctg
     6841 gacctgctgt gggacagcga gcgcgccggc cgcgtggaag gccagttgcg caccgccctg
     6901 gtggccggca gcgacgccga cggccgcacc cgctggtcct ggccgccgga cgccgcgctg
     6961 gccggcagcc tgcgtgcgca gctgccgcag atcgccgcct ggtcgccgct ggcgccgccg
     7021 ggctggcgcc tgggcggcgc gctggcggcc gagctgcaac tggccggcac gcgcgccgcg
     7081 ccgtcggtca ccggcagcct cagcgccgac ggggtggcgc tgcgctcggt ggtcgatggg
     7141 ctgcaattca aggacggccg gctgcgcgcg cggctggacg gtgcgcggct gcgcatcgac
     7201 gagttctcgc tggccggcgt cggtgcgtcg ggcagcggcg gccggctgat cgccagcggc
     7261 gaagccgcct ggcgcgacgg ccgtccgcag gcgcagctga acgccacgct tcagcggctg
     7321 cgcgtcagcg cgcgtgacga ccgccagctc agcgtctccg gcgcgctgca ggcggcgctg
     7381 gacggccgcg ccctgcgcac caccggtcgc ctgacggtgg atcaggcgct gatcgtgctg
     7441 cccgacgaca gccggccgac gctgggcgac gacgtgatcg tgcgcgggcc gggtggccag
     7501 gtgcgcgccg gccgacaggc cgacggccgc gcgccaccgc cgtcgggcgc ccccgacgcg
     7561 cctgcgcgca cgccgctgaa cgcgcatgtc gacgtgcaga tcgacctcgg ccccgagttc
     7621 cgcctgcgcg gcatgggcat cgacacgcgg ctggccggcc tgctgacgct gaccgccgac
     7681 ggcccgctga ccggcctgcc gcacgtccgc ggcacggtgc gcaccgtggg cggcaccttc
     7741 cacgcctaca gccagcagct gcgcatcgaa cgaggacaac tgctgttcca gggcgccgcc
     7801 gacaacccgg cactggacat cctggccctg cgcccgcagc tcggccacga ccagcgcgtg
     7861 ggcgtgcagg tggccggcac ggcgctgctg ccgcgcgtgc gcctgtactc ggcgccggcg
     7921 ctgcccgaca accagacgct ggcctggctg ctgctgggcc gcccggcgcc cgacaccggc
     7981 gccgaagccg ccatgctgca gtcggccgca ctggccctgc tgggcgggcg cgagggccgc
     8041 gggctggcgg cacgcttcgg gctggacgag ctgagctttt ccggcaccgg cgacggcgag
     8101 ctgaaccagg ccagcgtgac cctgggcaag cgcctgtccg agcgcctgta cgccgcctac
     8161 gagcacagca tcgccggcac cggcggcacg ctgatgatct tctacgagct gtcgcgccgc
     8221 tggtcgctgc gcggccaggc cggcgccgag aactcggcgc tggacctgat cttcaagctg
     8281 tcgttcgact gagcccggtg gacggccagc gcgccagcgc cgtcaaccgc cgggggcggg
     8341 gctgaggcgc tggcgcagcc agtcgggcag cggggccgat ttctgcgccg gaaagttgac
     8401 ccacaccacg gtggcgccgc cggtggcgca caccacgccg ggctggtcgg tgcgctccat
     8461 ggtggcccag gtgtcgaacg agctgcgacc caggtggctg acgaaggtct tgatcaggat
     8521 gtcaccgggg taggtgaact ggcggatgaa gttgcagaag gcgttgacga tgaccacgcc
     8581 ctcgccgccc ggatcgggct ggcagccgat gccgtgcagc cagtcgatgc gcgccgtctc
     8641 catgtagcga aagtacaccg cgttgttgat gtgccccatg gcgtccatgt caccccagcg
     8701 gatcgggatg gtcatttcaa acaccagttg cttgtgttcg ggaagctcga ttttcatggt
     8761 ctggcggcgg cggcgccggt gcgccgacag cctgggtgaa ctattgaaat gatagctgct
     8821 ggcgcttgct gcaaaagcgt cagcaaccga tcagagtgca aaaccgtcgt cggccgcgat
     8881 caccgcgccg ttgacgaaat ggctctggtc cgaagccagc atcatcagca ccgcgtccag
     8941 atcggccggc tcgcccacgc gcttgcgcgg cagcatgtcg atcagtttcc tgcccttctc
     9001 ggtctgccag tggtggtggt tgatctcggt gtcgatgtag ccggggcaga tggcgttgac
     9061 gttgatgccg tggcgccccc attccagcgc catggcgcgg gtcatctgga tcaccgcggc
     9121 cttgctcatg cagtacacgc cgatctgcgg cagcgcgcgc aggcccgcca tgctggcgat
     9181 gttgatgatg cgcccgccgg tgaagctgcc cggcgcggcc ccgcgtgcgc gcgccagcat
     9241 gcgcttgccc acttcctggg cgacgaagaa ggcgccgcgc acgttggtgt cgaacacgaa
     9301 gtcgtagtcc tgctcgctca cgtcctggat gcgctgggtg gtggacacgc cggagttgtt
     9361 gaccaggatg tcgatcgagc ccatttcggt ctcggcgtgg gccaccgcgg cgcggatgga
     9421 gtgcacgtcg gtcacgtcgc actcgaccac atgggcatcg ccgccgtcgc cctcgatgcg
     9481 cgcgcgcagg tctttcagct tctccaggcg tcggctggcc agcaccacgc cggcgccggc
     9541 cctggccagg gtgcgcgcga actgcgcccc caggccgccc gaggcgccag tgaccagggc
     9601 cacgcggccg gacaaatcga tgctgtagct catggaaagc actccggatc agaaagggtg
     9661 aggaaccagg gagacagacc gcccgccggg gcgcgagcgg cgctgcataa aatcgcctgc
     9721 acggccgacc ggcccggtgc gggcgcgcga cgaccatgcc gcccattatt tcaaaccccc
     9781 acgaggaagc gcccatgacc cccgagcaga tcatccagca gtacggcccg cgcgaagcca
     9841 tggagtacga cgtggtcatc gtcggtgccg gaccgtccgg cctgtcggcg gccatccgca
     9901 tcaagcagcg cgccgccgag cagggccagg aggtgtcggt ggtggtgctg gaaaagggca
     9961 gcgagccggg cgcccacatc ctgtcgggcg cggtgatgga cccgaaggca ctgaccgagc
    10021 tgatccccga ctggaaggcc aagggcgcgc ccctgaacca gcccgtgacc gacgacgcct
    10081 acgtgttcct gagcgagacg ggcggcacgc gcgtgccggg ggtgctgctg ccgccattcg
    10141 cccacaacca cggcaactac atcgtcagct tgggcaacgt ggtgcgctgg ctggccgagc
    10201 aggccgaggc gctgggggtg gagatcttcc ccggctttgc cgccgccgag gtgctgttca
    10261 acgacgacgg cagcgtcaag ggcgtggcca ccggcaacat gggcgtcggc aaggacggcg
    10321 aaccgaccga gaacttccag ctcggcatgg aactgcacgc caagtacacc atcttcgccg
    10381 aaggcgcgcg cggccacctg ggcaagcagc tgatcgcgcg cttcaagctc gacgcgcaga
    10441 gcgacccgca gagctacgcc atcggcatca aggagctgtg ggaaatcgac cccagccgcc
    10501 acacccccgg cttcgtcatg cacaccgccg gctggccgat ggacgagcac acctatggcg
    10561 gcgccttcct ctaccacgcc gaggaccaca aggtctactg cggctacgtg gtgggcctga
    10621 actaccgcaa cccctacctc agcccgttcg aggagttcca gcgctggaag acccacccgc
    10681 gcatcaagtg gtacttcacc gacgaccgcg gccaggtcaa cgccaagcgc ctgtcctacg
    10741 gcgcgcgcgc catcaccgcc ggcggcgtgc tggccctgcc gcggaccatc ttcccgggcg
    10801 gtgcgctggt cggctgcgac gccggctacc tgaacgtcag ccgcatcaag ggcagccacg
    10861 ccgccatcaa gaccggctcg ctggccggcg aagccgcctg cgacgccgtg ctggccgggc
    10921 gccagcacga tgaactcacc gcctaccccg aggccttcga ccacagctgg ctggcggccg
    10981 aactcaacaa ggaccgcaac ttcaagaact ggttcaagaa gggcctgacg gtcggcacgg
    11041 tgatgaacgg cttcgagcag tacgtgctgc gcggccacgc accctggacg ctgcaccgcg
    11101 ccgaaccgga ccatgtgcag ctgaaggccg cagccgagtg cgcgccgatc gactacccca
    11161 agcccgacgg caagctgacc ttcgaccgcc tgtccagcgt gttcatcagc aacaccaacc
    11221 acgaagaaaa ccagccggcg cacctgacac tgaaggaccc gggcgtgccg gtcaacgtca
    11281 acctggcgaa gttcgccggc ccggaaggcc gctactgccc ggccggggtg tacgaattcg
    11341 tgcccgacga agcccgcggc ggcaacgccc agcggctgca gatcaacgcc cagaactgcg
    11401 tgcactgcaa gacctgcgac atcaaggacc cgacgcagaa catcgtctgg gtcacgcccg
    11461 aaggcggcgg cgggccgaac tactccggca tgtgagcgcc tgcccgggcc gccaggccgg
    11521 gccatggcgc cggaggccgg ttcaggccag gtagggacgc agccagccca ggccgtcgct
    11581 ggtgccaccc ggcaccgcgc cacgcgccgg gcggtactcg cagccgatcc agccttcgta
    11641 gcccagccgg tcgagcagct cgaacaggta ggggtggtga atttcgccca ggtccggctc
    11701 gtggcgttgc ggcacaccgg cgatctggat gtggcccaca cgacctcctg gcagatggtg
    11761 gcggatcttc atcgccagat cgccctcgac gatctggcag tggtacaggt ccatctgcac
    11821 cttcacgttc ggccggtcga tggcgtcgag cagcgcgtgg gcctggtcct ggcgctgcag
    11881 atagaagccg ggcatgtcgc gcgggttgat cggctcgatc aggatgtcga tgccgtgcgg
    11941 cgcggcctgc tcggcggcgc cgcgcaggtt ggcgacatag gtggcgcgca gcgtggcggc
    12001 gtcgacaccc gccggcatca aaccggccat cacgtggatg cgcgggcaat ccagcgcgcg
    12061 tgcataggcg atggcgcgct ccaggccggc atggaattcg ccttcgcgcc ccgggatgca
    12121 ggccaggccg cgttcgcccg cgtcccattc gcccggcggc gcgttgaaca acacctgccg
    12181 cagcccgtgc tggcgcagca gcgcgctcag ttgctctggc gcccaggcgt agggaaacag
    12241 gtattccacc gccgtgaaac cgtcgctggc ggcagcagca aagcggtcga ggaaatccag
    12301 ttcgttgtag agcatcgaca ggttggcggc aaagcgcggc atgggtggtt cctcaccaga
    12361 aggcgttgaa ggcgcggcgc agttcgtcga tctgcgccgt gttcaggggg gcggccggtg
    12421 ccagcaacca cagccgggcg gtttcttcca gctcctccag cgtggccatg gcggcggccg
    12481 gggtgtcgtg ccagacgttg gggcccagcc gcgccagcat cacggcgcgg atcgggtgac
    12541 cggcttcggc atgccgggcg atggcccggg ccacggcctc ggcggcctcg ggcgcgccag
    12601 gccggtggta ggggatatgg ggcacgcggc cgaccttcat cacgaaatag ggcgtgagcg
    12661 gcggcagcag ttcggccgcc gccgggtcgg cccgcagcga gcaggccacc agatgggtgc
    12721 tgtggctgtg gaccacgcag ccggccggcg ccgccgtgcc ggcgctggcc tcgtagatgc
    12781 gccggtgcag cgccagggtc ttgctggcgg ggtcgccgcc ggtctggcgc ccctgggcgt
    12841 ccagtcgcgc cagtcgggca gggtcgagtg tgcccaggca ggcgtcggtg ggcgtgatca
    12901 ggtagccgtc ggccagccgc acgctgatgt tgccggccgt ggcatgcacg tagccgcgct
    12961 cgaacagcga gcggccgacg cgcacgattt cctcgcgggc ccgggcctcg ctcatgggcg
    13021 cggggctcat ggccgcagca ccgcgaacgc ccgggtgaag aaatcctcac caccgaaatt
    13081 gcccgatttg agggcgatgt gcaggccctg cggcgccagc ggggatgccg cgtggcacca
    13141 tggcacgccc gggtcgatct gcgcgccgat gcgcagttgc gcgacgccca gcgcctgcac
    13201 gcaggcgccc gaggtttcgc cgccggccac caccagctgg cgcacgccca gcccgaccag
    13261 cccgcgcgcg atggccgcca gggtctgctc caccagctcg cccgcccgcg ccacgcccag
    13321 gcgctgctgg atggcctgca cggcggccgg ctcggcggtc gaatacacca gcagcggcgc
    13381 gtccgcggcc agccgtccct gcgcccagtc gagcgcctgg cgcaccaccg cgtcgccggc
    13441 ggccatgcgc agcggatcga gcgccagcgc cgggtgaccg caggcgatga aatgcgccac
    13501 ctgggcctgg gtggcctgtg agcagctgcc cgccagcacc gcccgcaggc ccgacgcggc
    13561 gggcagcgcg gcggcctgcg ccgccggcgc cagtccgaaa ttggccggca gcgcgatcgc
    13621 cacgccggag ccggcagtca ccagcggcag ctccgccagc gccgggccga gccgccacag
    13681 gtcatcgttg gacaccgcat cgacgatggc gatgcccacc ccttcggcgc gcaggtcggc
    13741 aatgcgggcg cggatggcgt cgctgccggc cgccagcgtg cgctgctcga tcaggccgac
    13801 ccgccggcgc gtctgcgcct gcagcacgcg caccaggttg gcgtcggtca tgggcgtcag
    13861 cgggtgctgg cgcatgccgc tctcgctcag cagctggtcg ccgacgaaca ggtaaccctt
    13921 gaacacggtg cgcccgttgt cgggaaaggc cggcgtggcg atggtgaagt cgcagtccag
    13981 cgcctgcatc agcgcctcgg tgacgggccc gatgttgccg gccggcgtgc tgtcgaaggt
    14041 ggagcagtac ttgaagtaga tctggcgcgc accctgcgcc cgcagccagc gcagcgccgc
    14101 cagcgactgc gccaccgcct ggtcggcggc gatggtgcgc gacttcaggg ccaccaccac
    14161 ggcgtcggcg gcggcgtcgg cggccagcgg cgcctcgggc acgccgatgg tctgcaccac
    14221 gcgcatgccg gcgcgcacca ggttgttggc caggtcggtg gcaccggtga agtcgtcggc
    14281 gatgcagccc agcaggattg ggggcgtcat gggcgctcct tgtcggtcgg cgccggcagt
    14341 tcgatgccgg gaaaaatctt gatcaccgcg ctgtcgtcct ccagcgcgtg gccggcgctg
    14401 gaagcctgca tgaacatctg gtgcgcagta gccgccagcg gcagcggaaa cttgctggcg
    14461 cgcgccgtgt ccagcaccag gcccaggtcc ttgacgaaga tgtcgaccgc cgacagcggc
    14521 gtgtagtcgc ccgccagcac gtgggccatg cggttttcga acatccagct gttgccggcg
    14581 ctgtgggtga tgacctcgta caaagccgcc gggtccacgc cctcgcgcag gcccagcgcc
    14641 atggcctcgg cggcggcggc gatgtgcacc cccgccagca gttggttgat gatctttact
    14701 tggctgccgg caccggcgcg ctggccgagg cgatagacct tgccggccat ggcctccagc
    14761 accgcatcgg ccgcggcgta ggcggccggt gtggcggccg tcatcatggt catctggccg
    14821 ctggcggcac gcgccgcgcc gccagaaatc gggccgtcca cgtacaggat gccatgctgt
    14881 gccaagcggg cttcgagggc catcgaccag ttgggatcga cggtggagca catcacgaac
    14941 acgctgcccg gcgccatggc cgcagcagca ccatcgctgc cgaacagcac ctgctccgtc
    15001 tgggcggcgt tgaccaccac ggagaccacc acgcgcacgc cgttcgccac ctcagccggt
    15061 gtggtgcagg ccacgccgcc ctcggcagca aaggccgccg ccacgccggc acgggcgtcg
    15121 caggcacgca cccgatagcc gcgctgacgc agcgtgcagg cgatgcccag gcccatggcg
    15181 cccaggccga tcacgccgac gtgtcgatca ctcatggaag ggttcctcgt gccggtgcct
    15241 gccggtgggt ccggcggcag ccgggattct gacccgaaac ccggcctgcg cgccacgcgc
    15301 cggccgcacc gggccggcgc ctatcatcgg catccgcacg gcatacctgc cccgccgtgc
    15361 ctgcaccgag gagacgaaca tgcacatcgt gatcaccggc ggcgccggct ttctgggcgt
    15421 gcggttggcg cgcgcactgc tgcagcgcga cgcgctgtcg ctggcgggtg ccgcgccacg
    15481 cccgctcacc cggctgaccc tggtcgaccg cgtggcgccg ccggcggcgc tgacgcagga
    15541 cccgcgtgtg cgcgtggtga ccggcgagct ggacgcgctg ctgacgccgg acgccagcgg
    15601 tcagacgcgc gccgtgccga cggacagcga cgccgtggtg catctggcgg cggcggtgag
    15661 cggcgaatgc gaggccgact tcgacctcgg cctgcgcagc aacctggacg ccacgcgcgc
    15721 cctgctgcag acctgccggg ccctgggcac gcggccgctg ttcgtgttct cgtcctcgct
    15781 ggcggtattc ggccacacgc cggagcagcc gatgccggcc atcatcgagg acgacaccct
    15841 gccgacaccg caaaacagct atggcacgca gaagtacatg agcgagctgc tggtggccga
    15901 ctgcacccgc aagggtttcg tgcgcgggcg caacgtgcgc ctgatgacgg tgagcgtgcg
    15961 gccgggccgg cccaatgcgg cggcgtccag cttcctgtcg ggcatcgtgc gcgaacccct
    16021 ggccggccag cgcgccgtgt gcccggtggc gcccgacacc cgggtggcgc tgtcgtcgcc
    16081 cgcgcgcacc atcgaaggcc tggtgcgtgc gctggaggcc agcgacgaag actggggacc
    16141 gcgcaccgcg ctcaacctgc cggccctgtc gaccaccgcc ggcgacatgg tggcggcgct
    16201 ggcgcgcgtg gccggccccg aggtggcggc ccgggtggac tgggtggccg acgccggcat
    16261 cgcccgcatc gtcggcgcct ggccggccgg catgaacgcg cgccgcgccg cgcggctggg
    16321 cctgcgggcg gacgccagct tcgacgacat catccgcgac cacctgcgcg aaaacccggt
    16381 gccgcccgca gcagcggcac ggccataatg accggccgtg gtcgccgccg ctcggggcgt
    16441 gccgaccggc attcatcatc gatctgagga gtcgtatcca tgaagttgca ccaactggcc
    16501 ctgggcctcg gtctggcact gggactggtg gcgggcgcgc aggcgcagac caccatgaag
    16561 atcagcattt cggtggcgca gaactcgcac cagggcatcg ccatcgacac cttcgccaag
    16621 gaggtcgaaa agcgcaccgg cggtcgctac aagatccaga ccttctacag cggcgccctg
    16681 ggcggcgagc gcgaatcgat cgaggcggtg caactgggca cgcaggagct ggcgttcagc
    16741 tccaccggcc cggtgcccaa cttcgtgccc gaggtcaaga tcctcgacgt gcccttcctg
    16801 ttccgcgaca aggcgcatgc gcgcgccgtg ctggacggcc cgatcggcca ggacatgctg
    16861 gccaagttcg agcccaaggg cttcaaggcg ctggcctggg ccgagaacgg ctttcgccac
    16921 atgaccaaca gcaagcgcga cgtcaagacg ccggaggacc tcaagggcct gaagatgcgc
    16981 accatggaga acccggtgca cgtggcggcc tacaagggct tcggcatcat caccacgccg
    17041 atggccttcc ccgaggtgtt caccgcgctg cagcagggca cggtggacgg ccaggagaac
    17101 ccgctgtcgg tcatcatctc ggccaagttc gaccaggtgc aaaaacacct gacgctgacc
    17161 ggccacgtct actcgcccgc catcttcctg atgaacaagg gcgccttcga caagctgagc
    17221 gcggccgaca agcaggcctt catcgaagcc gccaaggaag gcaccaaggc caaccgcgcg
    17281 cgggtcgacg aggacgacgc caagggcgtg gccgacctgc gcgccaaggg catgacggtg
    17341 atcgacacca tcgacaaggc caagttcgtg tcggcgctgg cgccggtcaa tgcccagttc
    17401 gagaaggact tcggcaaggc caacctggac cgcatccgca acttcaagtg agctgccggc
    17461 ggcgctggcg tcgcctcgca gaatactatt gttttgatag cttctggcgc ccgactgtcc
    17521 tgcgcagcgg gcgtttttta tcataggttc gcggcatgaa agcgttcatc ctgaccctgg
    17581 agggctggac cacccgcatc gccatggtgc tggcctgtct gatgctggcg ctggccgcgg
    17641 cactgggggt gttccagatc ttcacccgct tcgtgctgga gcagccggcc gagtggagcg
    17701 aggtgctgat ccgcttttcg ctcatctgga tggtgttcct cggcataccg atggcgttcc
    17761 gtcagggcgc catggtgagc gtggacgcgc tctaccgcgc cgtgcccacc ggtgccaaac
    17821 gggtgctgga cctggtggtg gcgctggcgg cgctggcgct gatgctgatc gtgctgtggt
    17881 ggggctggga ctacgcggtg cgcggcagcg tgcagaccat ggcggggctg gaatcgctgt
    17941 cgatgttctg ggcctacctg gcgatgccgg tgggcgccgc gttctcgctg ctgggcattc
    18001 tggccaacta cctggatccg cgccgcatgg aactggagac cgcccaatga acgccgcccc
    18061 atgccgcttc gggtcaaccc gccggcccct cggccatgac agggcgcgcg gaggtgccgc
    18121 atgacttccg ccatgctgat caccatggtg ctgggctttg cgctcagcgt ctcggttgcg
    18181 gtgtcgatcg ggctggcggc catcgtcggc atccaggcca gtcaggcgca catgctgatt
    18241 tcggccaagg agatgttcaa cgccatcaac aagtttccgc tggcagcgat cccgtttttc
    18301 atcctggccg gcaacctgat ggaaaccggc ggcatctcgc gccggctggt ggaattcgcc
    18361 aagagcctgg tgggcggtgt gcagggtggc ctgcccatga cctgcgtgct gacctgcatg
    18421 atctttgccg cggtgtcggg ctcgtcggtg gccaccacct tcgccatcgg cgccatcctg
    18481 attccggcgc tcatcaagca cggctacccg accacctggg ccgccgcact gcaggccacc
    18541 agcgccgagc tcggcgtcat cattccgccc tcgattccga tgatcctgtt cggcgtctcg
    18601 gccgaggtgt cgatcggcga gctgttcatc gccggcttcg gccccggcct gctgatttcg
    18661 ggcgcgctga tgctgttcgt gtggctctac tgcaagtggc agggctgggg caggaacgac
    18721 ggcgacgggc gtctgggtgt cgtggccgcc acgcgccagg ccgcctgggc gctgctgatg
    18781 ccggtcatca tcctgggcgg catctacggc ggcatcttca cccccaccga ggcctcggcg
    18841 gtggccgtgc tgtacgccct ggtggtgggc atgctgatct accgcgaaat cggcgtcaag
    18901 gacctgtacg tgacgctgcg caagtcggtg gtgtcgtcgg cggtgatcat gttcatcatc
    18961 gccaacgccg gcctgttcgc cttcctgatc acgcgcgccg gcgtgcccga agccatcggc
    19021 cactggctgg aacaggtgct gcagaacccc gccctgttcc tgctgggcgt gaacgcggcg
    19081 ctgttcgtga tcggcatgtt catcgagacc agcgccgcca tcatcgtgct ggcgccgatc
    19141 ctggtgccgg tggcgcagcg cttcggcatc gacccggtgc atttcggcct ggtgatggtg
    19201 gtcaacctgg cgctgggcat gatcacgccg ccgttcggcg tcaacctgtt tgccgcctgt
    19261 accgtggcgc gcgtgtcgct cgacaagatc atccgccacc tggtgccctt cgtgggcgtg
    19321 gtgctggcct gcctgatggt gatcacctac gtccccacgc tgtcgctggc gctgcgcgat
    19381 ctggtgtacc ggtagtaccg gtaggcgtcg gggcgctaga atcgcgccgt caggagagag
    19441 cgcccgcagt ccaggcgccg ccgaaggccg aacgctcagg caaaaggact ggcacacatg
    19501 attctgctgg agagaggccg cccacaccgg cggcccaccg aaggggcaag ggccaaccgg
    19561 ctcgaatctc tcaggtaaag aggacagcag ggttccaggc ggggccagcg tgccttgccg
    19621 atgcccccca tcgcattcca ggagccccgc ccatgtccgc cgccgacagc cccctgctga
    19681 aaaccccgct gcacgccctg cacctcgaac tgggcgcgcg catggtgccc tttgccggct
    19741 acgagatgcc ggtgcagtac caggctggcc tgatcgccga acaccgccac acgcgcgagg
    19801 cggccggcct gttcgacgtc tcgcacatgg gccagctcaa gctgatcggc ccggatgccg
    19861 ccgcagcgct ggaaaccctg atgccggtcg acgtgcaggg cctggccgag aacaagcagc
    19921 gctacggcct gctgctgacc gacgagggca ccatcatcga cgacctgatg ttcgtcaacc
    19981 gcggtgacca cctgttcctg attgtgaatg gcgcctgcaa ggccggcgac atcgcccaca
    20041 tgcaggcgcg catcggcagc cgctgcctgg tgcagcccct gcccgatcag gccctgctgg
    20101 ccctgcaggg cccgcaggcc gtcggcgcgc tgcagcgcct ggtggccggc gtcgagaagc
    20161 tggtgttcat gaccggcggc gccttcgact ggctgggcac gcccttgtac gtgacccgca
    20221 gcggctacac cggcgaggac ggtttcgaga tttcgctgcc cggcgagcgc gccgaggcct
    20281 tcgcccgcgc cctgctggcg cagcccgagg tcaagcccat cggcctgggc gcacgcaatt
    20341 cgctgcgcct ggaagccgga ctgtgcctgt atggcaacga catcgaccac accaccagcc
    20401 cggtcgaagc cggcctgaac tgggccatgc agaaggtgcg caggagtggc ggcgcgcgcg
    20461 ccggcggctt tccgggggcg gcgcgcattc tggcccagct cgacggcacg cagccggtgg
    20521 aacgccagcg catcgccctg atggcccgcg agcgcgtacc ggtgcgcgaa ccggccgaac
    20581 tgcgcaacct ggacggccag caggtcggcc aggtcaccag cggcctgctc agcccgacgc
    20641 tcgacaagcc catcgccatg ggctacgtgc gccccggttt cgccaccatc ggcaccgagc
    20701 tgttcgccat ggtgcgcggc aagccggtgc ccatggaggt ggtggccacg cccttcgtgc
    20761 cgaaccgcta ccaccgcggt tgacccaagc aaaaccggcc gcagcgcccg tccaacctgc
    20821 gccagcaact catttttcag gagcaaacat gagcctcaag tacaccgaag accacgaatg
    20881 gctgaagatc gagggcgacg tcgccaccgt cggcatcacc caccatgcgc aggacgcgct
    20941 gggcgacgtg gtgttcgtcg agctgcccga ggtcggccgc cactatgccc agaaggacgc
    21001 ggccggcgtg gtcgagagcg tcaaggccgc cgccgacgtc tacatgccgg tcagcggcga
    21061 agtgctcgaa gtcaacgagg cgctgcgcga agaccccagc ctggccaaca gcgacccgca
    21121 gggcgccggc tggttcttca ggattcgcct gagcgagccg gcgcaggccg gcgcgctgat
    21181 ggacgaagcc gcctacaccg ccttcagcgc cggctgaagc ccgggcgcgc tgccgcccca
    21241 cccgcgccac gcccggagcg cggcgccgcg ccgccgacgc agggccaccc ccacggcctg
    21301 tgcttgcgcg cctgcagcgc acccgccgcg cccgcgccaa ccccctttcc ttcgccgctt
    21361 gcatcgatgc cacccatgag ccagcccacc ctgcccgaac tggaaaaccc cggtgaattc
    21421 atcgcccgcc acatcggccc cagcccggcc gaggaggccc acatgctgtc ggtcatcggc
    21481 gaggcctcgc gccgggcgct gatcgactcg atcctgccgc cggacatcgc gcgccgcacg
    21541 ccgatggatc tgccgccggc ctgcagcgaa gccgacgcgc tggccgaact caaggccatc
    21601 gccagccgca accggcacca gatgaagagt ttcatcggcc agggctacta cggctgccac
    21661 ctgccgggcg tgatcctgcg caacatcctg gagaaccccg cctggtacac cgcctacacg
    21721 ccctaccagg ccgaaatcag ccagggccgc atggaggcgc tggtcaactt ccagaccatg
    21781 gtgaccgacc tgaccggcat ggacatcgcc aacgcctcca tgctggacga agccaccgcc
    21841 gccgccgagg ccatgacgct ggcacgccgc accgtcaagg ccaagggcca ggtgatcgtg
    21901 ctgtcgggcg actgccatcc gcagaccatc gaggtcatgc gcacgcgcgc cgcgccgctg
    21961 ggcctgacgc tcaggctggc caactcggcc gccgagtggg acgccgccat cgccgccgac
    22021 gactactttg ccgccctgat ccagtacccc gccagcagcg gctggctgat ggactggagc
    22081 gacgaggtcg agaagatcca cgccaagggc gcggccgcca tctttgccgc cgacctgctg
    22141 gcgctgaccc tgctcaagac gccgggcgag atgggcgccg acatcgtggt gggcagcacc
    22201 cagcgctttg gcatgcccat gggcgcgggc ggcccgcacg ccgcctacat ggcctgccgc
    22261 gacgattaca aacgctccat gcccggccgc ctggtcggcg tcagcgtgga tgcgcacggc
    22321 cagccggcct accggctggc gctgcaaacg cgcgaacagc acatccgccg cgaaaaagcc
    22381 accagcaaca tctgcaccgc gcaggtgctg ccggcggtga tcgccagcat gtacgcggtc
    22441 taccacggcc ccgtcggcct gacgcgcatc gccgagcgca cggcacgcct gacggcgatc
    22501 ctgaaggccg gtctggcgca gctcggcttc acccacgtcc atcacgacag cgccttcgac
    22561 acgctctgcc tgaagaccga aggcgctaca gattcaatag ctgctcgcgc agttcagatg
    22621 ggcgccaacc tgcgaaaagc ctgggattcc tgcctgtgcg tgtcgctgga cgaaaccagc
    22681 acccgcgccg acgtcgagct gctgtggcgc atcttcgccg gcgacgccag ggcgctgccc
    22741 gggttcgacg cgctggccgc cacggcgccg gcgctgatcc cggccgcgct gcgtcgcacc
    22801 agcggctttc tcacccaccc ggtgttcaac acccaccatt ccgagacggc catgctgcgc
    22861 tacatccgcc agctgtccga caaggacctg gcgctggacc gcagcatgat cccgctgggc
    22921 agctgcacca tgaagctcaa cgccaccagc gagatgatcc ccatcacctg gccggaattc
    22981 gccaacgtcc acccgtttgc cccggccgac cagcgccagg gctatgccgc gctcgaccgg
    23041 cagctgcgcg cctggctgtc gtccatcacc ggctacgccg acgtcagcct gcagcccaac
    23101 gccggcagcc agggcgaata cgccggcctg ctggcgatcc gcgcctacca cgccgcgcag
    23161 ggccagggcc agcgcgacat ctgcctgatc ccggccagcg cccatggcac caacccggcc
    23221 agcgccatga tggtgggcct gaaggtggtg gtgacccaat gcgacgagca gggcaacgtc
    23281 gacctggccg acctgcaggc ccagtgcgaa aagcacgcgg ctcacctggc ctgcgtgatg
    23341 atcacctacc ccagcacgca cggcgtgttc gaaacccagg tcaagcggct gtgcgagatc
    23401 gtccaccagc atggcggccg cgtctacctg gacggtgcca acctgaacgc catggtcggc
    23461 ctggccgcgc cgggcgagtt cggcggcgac g
//
LOCUS       DAOOOG010000020        23273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_2106_length_23273_cov_27.830606, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000020 DAOOOG010000000
VERSION     DAOOOG010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 23273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23273
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2106_length_23273_cov_27.830606"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PK242_03080"
     CDS             complement(<1..620)
                     /locus_tag="PK242_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOZ93035.1"
                     /translation="MKIEVRDVPPRAAWALEQAGVHPLLARLYAARGVSSAAELDDGL
                     AQLLPPDRLQGAQAAAVLLADAIAAGRRICIVADYDCDGATACAVGLRGLRLLGATQL
                     DYLVPDRVIDGYGLTPPIAERVAARGAELLITVDNGIASVEGVARAKALGLQVLVTDH
                     HLPGGALPPADGIVNPNQPGCGFESKALAGVGVMFYVLLALRAELRA"
     gene            complement(617..1567)
                     /locus_tag="PK242_03085"
     CDS             complement(617..1567)
                     /locus_tag="PK242_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HOZ93036.1"
                     /translation="MSAAPAWPHVLIPDAVLALPDGAAAPPWPELPALRALARQLRLA
                     ERLTLDEDSPATPFEVALARAHGLPDTPGRTPWAAFESGTSGTPCAWLLPCHWQMGMN
                     DVSVLHPAELGLDERESRALLASVEPLLRDDGLTLRYLRPDAWLVQGELLRGLSCWSL
                     RRALTRPVTRQQLAQAPTEAQRRTLRRLQSEWQMLLYTHPVNEAREGARRWPVNALWI
                     GGAGELPQAIAPRADVQVCRELLDLPARLSLHDWQRAWQAIDANALAPLLQTVRGGAP
                     ARLTLCGPRRALTLSSAQGLTQKIMSQISPLRLSNLRDQL"
     gene            1634..2902
                     /locus_tag="PK242_03090"
     CDS             1634..2902
                     /locus_tag="PK242_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HOZ93037.1"
                     /translation="MSLAQSLPYEMLLGWRYTRAGRGTRRNGFISFISAASMLGIALG
                     VAALIVVLSVMNGFRKDVTERMLSVISHVEVFSPDGEALPDPARTLAEVRANPEVVGA
                     APFIGAQALLARGESMQGTIVRGIDPAREPFVTEVAAQLQATLLPRLVPGSYGILLGS
                     DLARKLGVAEGDAVTLIAPSGQVTPAGVVPRLKQMTVVGTFSSGHYEYDSALAMLHID
                     DAARIFRLQGPTGIRLKLRDLHRARSVADQLAASLSGHLLILDWTRQNRTWFAAVQLE
                     KRMMFIILTLIVAVAAFNLVSTLVMTVQDKRADIAILRTLGASPASIMKVFMVQGALV
                     GVIGTLGGLALGLAIAFHIDVIVPFLERLFHASFLPKDVYLISQMPSDPQAGDIVPIG
                     VISLLLSFLATLYPSWRASRVNPAEALRYE"
     gene            2895..3623
                     /gene="lolD"
                     /locus_tag="PK242_03095"
     CDS             2895..3623
                     /gene="lolD"
                     /locus_tag="PK242_03095"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HOZ93038.1"
                     /translation="MSEVAIENIAAGADPASAGIVLEAESLGRRFHEGPLDVTVLQGV
                     DLRVRAGETLAIVGQSGSGKSTLLHLLGGLDAPSSGRVRLLGQDLGSLSPAAQGELRN
                     RHLGFVYQFHHLLPEFSALENVAMPLWIRRQPRAEAAEAARHMLARVGLQERVRHRPS
                     ELSGGERQRVAIARALVTRPACVLADEPTGNLDRATADAVFALMMELAAQHGTAFIVV
                     THDAQLAGRCARVLRLVQGRLGPV"
     gene            3768..4502
                     /locus_tag="PK242_03100"
     CDS             3768..4502
                     /locus_tag="PK242_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOZ93039.1"
                     /translation="MESDQEVFARWERLIQFRLLADSQEPTRTVPEQVAAQVGDRIIG
                     GALAPGARIGEQELADEFAISRGPVREAIRILEREGLVQVLPRRGAVVTSLSAQELRE
                     LFEIRAGLVEIALRKVMGAGVPPELLAITRAGVARLEALAELPEGGDAYAETTYRLIQ
                     IGAHFAGNERLRRMVVALSLQSLRYSKLGLASVARRQRSVKLWRQALQALERGDVERT
                     LWLARQRSQESADEAARRLAEPSGGS"
     gene            complement(5127..6692)
                     /locus_tag="PK242_03105"
     CDS             complement(5127..6692)
                     /locus_tag="PK242_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ93040.1"
                     /translation="MIAGRLTTWAAAQHGARTAVVFGEQRYSHAEIEARSNRFAQAAL
                     ALGLQPGERLAVLLDNSVESLDSGFGAEKAGLTYVALNARHTLVEQLDILADAQASAV
                     LVGPRHGELAAAFAQAAGHQLPELRHVLGLGVDAPGVTDWRLATAAAPDHPPAIEIGP
                     DHLMRIAYTSGTTGRPKGVAYTLERWQARLANHFLAMEYGLSVDDAMLHVGPLTHAAG
                     VYLFPCFLRGARNVVLDQFDPATLLATIERERITHLMLVPTMMHRLVEAIEGGARGDW
                     SSLRRIHYGTAPTPVPLIRRAQAIFGPILRQQYGMTEAVQPLCVLYPHEHQGTSADGA
                     DEPIGSCGRPTANVRIVLRDTQGREVAPGEVGEITIAHEGIGKVQYWRRPELLAETVR
                     DGWYYSGDLGRLDRQGFLHIVGRNKDMIISGGFNVYAREVEDALSTHPAVLEAAVLGL
                     PDPQWGELVAAAVVLRADCTASAEALMAHCGERIAGYKKPRRIAFVEALPRNLAGKVQ
                     KPALRERFTSQGL"
     gene            complement(6689..6907)
                     /locus_tag="PK242_03110"
     CDS             complement(6689..6907)
                     /locus_tag="PK242_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOZ93041.1"
                     /translation="MPRIHLPSEVTGTVWKVEVSAGDPVSADQTLVILESMKMEIPLT
                     APRAGVVLELLVAEGESVAEGQTVVVLE"
     gene            7055..8044
                     /locus_tag="PK242_03115"
     CDS             7055..8044
                     /locus_tag="PK242_03115"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ93042.1"
                     /translation="MQFRFLAGLMLGGLCLAATALAQTAWKPSERINYIIGVAPGGTV
                     DLYARAIKDSLERLKLTGGEPVIVENKVGAGGALAMNALRAGHGNAHYLGTFHTGAIA
                     GAVTGLIKADPREFMPVAMLVEETSVVAVRADSPLRSGTDLVAALKADPTALRIAVAP
                     ALGQNIHMAIAKPLHVAGVAIDKLTIAPFRSSGESVTALIGGHVDVVSATAPVVLPQV
                     ANGKLRILASAAPVPGSGPLAGVKTWREQGVPADYVSYNGVTLPPGVHAEQIRFWEEA
                     LRKVSQDPQWRALVEKSGNQPIFRGYVESHRYMMSEWDAAAELARTLGLKGGR"
     gene            8041..9624
                     /locus_tag="PK242_03120"
     CDS             8041..9624
                     /locus_tag="PK242_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOZ93043.1"
                     /translation="MSAQQGQTLAPAGEWAAELAELQQRRASALAMGGAQALARMRDS
                     GRMNARERIAALIDQGSFHEMGTLAGKGHYDAQGRYSHTDPTNAIVGTGRIDGRKVAL
                     HVDDYSIRAGSSEGTIADKWIYIERLAHQLRMPLVRLVDSAGGSVKLLMQLGGTKIPE
                     YTTWPAQELLQTVPVVGVALGACAGQGAVKVLSSHFSVLVRGQAQVMAAGPHVVRQAY
                     GVEVDKNELGGHLAQRKSALVHNEAEDEADALAQVRRFLSYLPRSVHELAPVVACDDD
                     PHRADDWLKDAVPRDRRKIYDPRRILAAVFDQGSLFEIGRWQGGSLLTLLGRLNGVPV
                     GVIANDPKVQGGAMTLQAAYKMERHTRLCSQFGLPVVNFVDQPGNATGLEAELAGTLL
                     GAVRLGEALAHCRSPWVSILMRRCFGMAGALHGPKGGARLNHRFAWPSARWGSIPIEG
                     GVMAAHKAEIEAAPDPAARRAELEAHYLAMTSPFRTAEKFGVLDIIDPRETRRVLCDW
                     VEDAWYVVRGDLAQRSAGA"
     gene            complement(9704..10798)
                     /locus_tag="PK242_03125"
     CDS             complement(9704..10798)
                     /locus_tag="PK242_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOZ93044.1"
                     /translation="MNFAWPAFLKWPAAIPQPAFLWPHMLWLLALLPLLVLLYAWLLR
                     RRRKLALRYASLSIVRQAQGRGPGWRRHLPPLLLLLALAALLLASARPVAVIALPSNQ
                     QTIILAMDVSASMRATDVEPNRLVAAQEAAKTFLKELPRDVKVGIVAFAGSAQVAQQP
                     TLNREDLVTAIERFQMQRATATGNAIVISLATLFPHAGIELQQLSPDRMRGGWGRSLD
                     QPPPKQNEFVPVPPGSYSSAAIIMLTDGQRTTGVDPLDAAKMAAERGVKVYTVGIGTV
                     DGEIIGFEGWSMRVRLDEETLKTIAQKTAAEYFYAGTAQDLKKVYQSLSSRLTLEKKE
                     TEVSSLFALAGALLALLAGGLSLLWFGRVL"
     gene            complement(10820..11839)
                     /locus_tag="PK242_03130"
     CDS             complement(10820..11839)
                     /locus_tag="PK242_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOZ93045.1"
                     /translation="MLRWLRPRRPPPHAVPNPARADPQPGPAAADTEALLRRLEWTVL
                     RRLDGVLQGDVRTLMRGSGLDLADLREYQAHDDVRHIDWNVTARLQTPHVRVYTEDRE
                     MAAWFLLDLSPSVDFGSGQRLKREVLAEFVAVLARLLTRHGNRVGALLYGARVEQVIP
                     ARASRRHVLHLLHAIGHRPAPAPTAEAGTTRLAELLRAAGHLVHRRATLFVVSDFISA
                     PGWEQPLAQLASRHDVVAVRLLDPLERALPDLGLMTLRDAESGEQMLVDTHDAGFRQR
                     FAALAARHEDELRQTLARAGVDALELSTEGALTDAIVRFVELRRQRARLAAGEGLPAH
                     LRKAG"
     gene            complement(11874..12893)
                     /locus_tag="PK242_03135"
     CDS             complement(11874..12893)
                     /locus_tag="PK242_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOZ93046.1"
                     /translation="MTDKDNPRLETDPATAELMERILYEVKRVVVGQDRFLERVMVAL
                     LAGGHLLVEGVPGLAKTLTVKTLAAVLQGQFRRIQFTPDLVPADLIGTRIYNQKTGEF
                     STALGPVFTHLLLADEINRAPAKVQSALLEVMQERQVTIAGQTHFVPEPFLVMATQNP
                     IETEGTYPLPEAQVDRFMMKVLVDYPSDEEEFVIAQRVTGPRIEVQAVASMEQLAALQ
                     QQCRQVYVDPSLTQYAVRLVAATRRPADVGLKELAPMLGYGASPRATIGLIEGARALA
                     LMRGRAYALPEDMSDLVPDVLRHRLVLSYEALSEGLSADQIVLRLMAKVAPPAKPLAH
                     EPLAA"
     gene            complement(12919..13839)
                     /locus_tag="PK242_03140"
     CDS             complement(12919..13839)
                     /locus_tag="PK242_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOZ93047.1"
                     /translation="MRRSALYSRSSPAPVVAPDAPAPAPALAPERARRRPHLGAALRH
                     PGALWAAVLALALALALGLDLGAWRRPAPRQLTQKDIDDAVLHTATHKVLPSAAARAA
                     AAVAPSLVRVEGVVEQKKGRKGQPELGRGIGTGVVIIDNGTILTNLHVVQGAERITVT
                     FADGLESEAGIVNMQPENDLAVLRAHKLPDDLQAATMRSTGDLAPGDEVVAIGYPFGI
                     GPSVSAGVVSGLGRGFRSPDGGQLMRNLIQFDAAANPGNSGGPLVTMDGQVVGIVTAI
                     LNPTPARTFLGIGFAVPIENAAAGAGQIPF"
     gene            13924..14325
                     /locus_tag="PK242_03145"
     CDS             13924..14325
                     /locus_tag="PK242_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93048.1"
                     /translation="MGAMTVLRALLVWVLLLLLAIANGVLREAVLIPRLGGPSGLVLS
                     GLLLSALILVLAWWLLPWIGVRGHGPLLLTGLGWLALTLGFEFTFGLWRGRTLAELLA
                     AYRFEGGNLWSLVLLVTAGAPWLAGRLRRCT"
     gene            14353..15207
                     /locus_tag="PK242_03150"
     CDS             14353..15207
                     /locus_tag="PK242_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOZ93049.1"
                     /translation="MAAWIDTHCHLDAPEFSADVAAVRARAATENVAICVIPAVEPAA
                     FEAVRALAHRFGDAYALGIHPMYVARAPDDALARLEAALARHADDPRLVAVGEIGLDL
                     FVPELGVAPLRARQQHFFIEQLRLARRHDLPVLLHLRRAVDAVLQVLREVGVAGGIAH
                     AFSGSRQQADQLLALGFRLGFGGAVTYPRASRLRALAAELPASAIVMETDAPDIPPHW
                     LYTTAEARAQGQPQGRNEPGELPRIGAELAALRGVSAADWADLSTANALAALPRLRRL
                     WGAGEHDR"
     gene            15587..17041
                     /locus_tag="PK242_03155"
     CDS             15587..17041
                     /locus_tag="PK242_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ93050.1"
                     /translation="MTTTVDTPAGSPAATPTADAAPRRRGLGVTLTLLLIAALVGSAW
                     WLVQRARAPEAAAPAAAPGGAGGPGPGSASVTVGAAHAQRGELPIVIDALGTVTPPVT
                     ATLVPQVSGVLTEVLFTEGQMVSKGQVLARIDARPYEQALAQARGQRAKDEAQLAAAR
                     VTLARYQTLWQQDSIARQDVDTQAALVKQLEGVVEADRASERAAQLNVGFASIRAPIG
                     GRIGLRAIDPGNLVSTGSAGGIATITQIAPIDVVFSVPQDRIAAVLAAQRRGRLPVQA
                     FDRARSQPLAEGSFLTLDNQVSTATGTVRAKARFANADGALFPNQFVNARLQLGHDSG
                     VLVPVTAVRTGPQGDYVYVIDDERVARMRPVRRGMATVDQVLIVQGLQAGERVVTEGG
                     DRVKDGGKLRLAGESPGPGGAAGMRPGAAPAPAPTAPAAIASGAGEAGAAAPPWLERL
                     PPELRDKVRAMSPEERRAFLQQRRAAASAADSGR"
     gene            17068..20241
                     /locus_tag="PK242_03160"
     CDS             17068..20241
                     /locus_tag="PK242_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ93051.1"
                     /translation="MNVSRLFIQRPVATALCMLAIVLAGLLGLRLLPVAALPQVDYPT
                     IQVQTLYPGGSPDVMSRTVTAPLERQLGQMSGLERMSSVSSAGVSLITLQFALHLNMD
                     AAEQQVQAAMNAAATLLPADLPTPPVYAKVNPADAPILQLAVTSDSLPLPEVQNMVNT
                     RLALKISQINGVGLVTLAGGQRPAVRVQANLQALAAMGLGLDAISSAISAGNSSSAKG
                     SFDGPTRQYTINANDQLLTAHEYRELIVAFVAGQPVRLKDVAEVVDGAENRRLGAWVA
                     IKNDSASRTPDARQSPGTSEIELPLTPAIVLNVQRQPGANVIATVDAIRRQLPELRQS
                     LPDSVRLTVLSDRTQGIRASVAHVELELGLAVVMVVLVIFAFLHSARATLIASIAVPI
                     SLAGTLAAMVLLGYSLNNLSLMALTIATGFVVDDAIVMIENISRHRELGKPPLQAALA
                     GAGEIGFTIISLTVSLIAVLIPLLFMQEVIGRLFREFAVTLAITILFSALVSLTLVPM
                     MSARWLEPLGQAHGPVGARMQAWLDATIAGYDRALRWVLARQPLTLALALLTLAVTVL
                     LYAGMPKGLFPTQSTGQLQGLLQAAPEVSFERMSALQGAAAQVVLRDPAVRSVSSVVG
                     VDAANNSALASGRLVINLRPRTPWGDTQATVMQRLRRQVGEQVAGVTLYLQPTQDLTI
                     DAVSGPTAQRFDLEGVDTALVDGWARRLVARLQRLPELRHVTTDAGATGLAAMVRVER
                     DTAARLGVTASNLDNALYNAFGQRIVSTIFSETNQYRVILEAARAVDASLRTLRELPV
                     RTSVGGTTPLEAFARVVEEPSPLQVQRVDQYPAATVGFDTAPGVSLGAAVSAIEAAVR
                     AEGLPAALTLRMTGAAGAFQQSLGNQLWLILAAVVCVYIVLGVLYESYVHPLTILSTL
                     PSAGVGALLALWLTGHPLDVVGIIGLVLLIGIVKKNAIMMIDFAIEAERAGKSALEAI
                     HQAALLRFRPILMTTLAALAAAVPLMLSWGDGGELRRPLGIAIFGGLVLSQLLTLFTT
                     PVIYLWFDRLGQRLAGRAPVGEVGHAD"
     gene            20393..>23273
                     /locus_tag="PK242_03165"
     CDS             20393..>23273
                     /locus_tag="PK242_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ93052.1"
                     /translation="MNLSRPFIHRPIATVLLTLGLALAGMAAYFVLPVARLPAVEFPV
                     IFVNASMAGASPADMARTVATPLERTLGTISGVNEMTSSSNTGSTRVVLQFDLGRDID
                     SAARDVQAAINAARIDLPASLRSNPTYRKANPAAQPFMTLALTSTTRTPGQIYDAVSN
                     IVSQRLQQVDGVGDVELGGGSLPAVRVQLNPFALHELGIGAEDVRAAIQAHNANRPKG
                     VIEVGGSADGRALQVLTPAPGLRAADYQDLIVALRDGHEVRLSQVARVIDSVQDTRTR
                     GMFNGQPAIVVNITQQPTANLIETADAIDALLPQLREQLPSDIELHVAVDRTGSVRAS
                     VREVEITLVIAVLLVVGVVGLFLRTLRAALIPAVATVVSLLGTLGVMYVLGFSLNNLT
                     LMALTVATGFVVDDAIVVLENITRHLEQGVARRRAALQGAREVGFTVLTISVSLVAVF
                     IPLLFMGGQVGRMFREFAVTLSAAVLISLLISLTTTPMLCALLLRPPAAGTPPRQSVA
                     GRLRQRLGGWARGSQRSLTRAYAGALDWALASPWLVVAVLLVVIGLNGYLFSAITKGF
                     FPDQDNGLMTGGLRADQSISSGAMADKLQQAVDILRADPSVGTVVAFSGGWRSGGGFL
                     SAALKPPGQRPPGESTRAVIQRLRPQLGRITGLRVFLNPVQELRIGGRSSNSSYQYTL
                     KTDNQADLRNWTRRLSEQMKRDARLTEIDDDQSEHSVETFVQIDRERAARLGVALPGI
                     DATLYDAFGQRQVATMFSALNQYAVVLEWAPEHARAPQALHDVMLPVAPAVSARLAGQ
                     AAPVASATSANPAQRGASTGQPISFTPAGMVPLPAIAEFSERAVPTSVWHDGGELSST
                     LSFDLADGVSIEQGRQAVLDAVDAVGLPNNVRGVFSGAAAQAQQTQAQQVLLIGAAIV
                     VIYIVLGILYESLIHPITVLTTLPSAGFGAVLALLLLKMEFSIMAL"
BASE COUNT         3193 a         8095 c         8557 g         3428 t
ORIGIN      
        1 gcgcgcagct cggcgcgcag cgccagcagc acgtagaaca tcacgcccac gccggccagc
       61 gccttgctct cgaagccgca gcccggctgg ttggggttga cgatgccgtc ggccggcggc
      121 agcgcgccgc ccggcaggtg gtggtcggtc accagcacct gcaggcccag cgccttggcg
      181 cgcgccacgc cttcgacgct ggcgatgccg ttgtccacgg tgatcagcag ctcggcgccg
      241 cgcgcggcca cgcgctcggc gatcggcggc gtcaggccgt agccgtcgat cacgcggtcg
      301 ggcaccagat agtccagctg cgtggcgccc agcaggcgca ggccgcgcag gccgacggcg
      361 caggcggtgg cgccgtcgca gtcgtagtcg gcgacgatgc agatgcgccg cccggcggcg
      421 atggcgtcgg ccagcagcac ggcggcggcc tgcgcgccct gcagccggtc gggcggcagc
      481 aactgcgcca ggccgtcgtc gagctcggcg gccgaactga cgccgcgcgc tgcgtacagg
      541 cgcgccagca gcgggtgtac gccggcctgc tcgagcgccc aggcggcgcg cggggggacg
      601 tcgcggactt caattttcat agctggtcgc gcagattgga caagcgcaag gggctgattt
      661 ggctcatgat cttctgggtc aggccctggg cgctcgacag cgtcagcgcg cggcgcgggc
      721 cgcacagcgt gagccgcgcc ggggcgccgc cgcgcaccgt ctgcagcagc ggggccagcg
      781 cgttcgcgtc gatcgcctgc caggcgcgct gccagtcgtg caggctcagg cgggccggca
      841 gatcgagcag ttcgcggcac acctgcacgt cggcccgcgg ggcgatggcc tggggcagct
      901 cgccggcgcc gccgatccac agtgcgttga ccggccagcg ccgcgcgcct tcgcgcgctt
      961 cgttgacggg gtgggtgtag agcagcatct gccattcgct ttgcaggcgg cgcagcgtgc
     1021 ggcgctgcgc ttcggtgggc gcctgcgcca gttgctggcg cgtgaccggc cgggtcagcg
     1081 cccgccgcag cgaccagcag ctcaggccgc gcagcagctc gccctggacc agccaggcgt
     1141 cggggcgcag gtagcgcagc gtcaggccgt cgtcgcgcag cagcggctcg acgctggcca
     1201 gcagggcgcg cgattcgcgc tcgtccagcc ccagctcggc cgggtgcagc acgctgacgt
     1261 cgttcatgcc catctgccag tggcagggca gcagccaggc gcagggggtg ccgctggtgc
     1321 cgctttcgaa ggcggcccag ggcgtgcgtc cgggggtgtc gggcaggccg tgggcgcgcg
     1381 ccagcgccac ctcgaacggg gtggccgggc tgtcttcgtc cagcgtcagg cgctcggcca
     1441 ggcgcagctg gcgcgccagg gcgcgcagcg ccggcagctc gggccagggc ggcgccgcgg
     1501 cgccgtcggg cagggccagc acagcatcgg ggatcaggac atgcggccag gcaggtgcag
     1561 cggacatggc cgcattgtcc gggatgccac aatgccgggc ttgtcctgca cggcgtgccc
     1621 cgccttccac ccgatgtccc tggcccagag cctgccctac gaaatgctgc ttggctggcg
     1681 ctacacgcgc gccgggcgcg gcacgcggcg caacggcttc atctcgttca tctcggcggc
     1741 gtcgatgctg ggcattgcgc tgggcgtggc ggcgctgatc gtggtgctgt cggtgatgaa
     1801 cggctttcgc aaggacgtca ccgagcgcat gctcagcgtg atttcgcacg tcgaggtgtt
     1861 ttcgcccgac ggcgaggcgc tgcccgaccc ggcgcgcacc ctggccgagg tgcgcgccaa
     1921 ccccgaggtg gtgggcgccg cgcccttcat cggcgcgcag gcgctgctgg cgcgcggcga
     1981 gtccatgcag ggcaccatcg tgcgtggcat cgacccggcg cgcgagccct tcgtcaccga
     2041 ggtggcggcg cagctgcagg ccacgctgct gccgcgcctg gtgccgggca gctacggcat
     2101 cctgctgggc agcgacctgg cgcgcaagct gggcgtggcc gagggcgatg cggtgacgct
     2161 gatcgcaccc agcggccagg tcacccccgc cggcgtggtg ccgcgcctca agcagatgac
     2221 ggtggtcggc accttcagca gcggccacta cgaatacgac tcggcgctgg ccatgctgca
     2281 catcgacgac gcggcacgca tcttccgcct gcaggggccg accggcatcc gcctgaagct
     2341 gcgcgatctg caccgggcgc gcagcgtggc cgaccagctg gccgcgtccc tgagcggcca
     2401 cctgctgatc ctcgactgga cgcgccagaa ccgcacctgg ttcgccgccg tgcaactgga
     2461 aaagcgcatg atgttcatca tcctcacgct catcgtcgcc gtggccgcct tcaacctggt
     2521 cagcacgctg gtgatgacgg tgcaggacaa gcgcgccgac atcgccatcc tgcgcaccct
     2581 gggcgccagc ccggccagca tcatgaaggt gttcatggtg cagggcgcgc tggtgggcgt
     2641 gatcggcacc ctgggcgggc tggcgctggg cctggcgatc gccttccaca tcgacgtcat
     2701 cgtgccgttc ctcgaacgcc tgttccacgc cagcttcctg cccaaggacg tgtacctgat
     2761 cagccagatg cccagcgacc cgcaggccgg cgacatcgtt ccgatcggcg tcatctcgct
     2821 gctgctgtcg ttcctggcca cgctctaccc gagctggcgc gccagccgcg tcaaccccgc
     2881 cgaggcgctg cgctatgagt gaagtagcta ttgaaaacat agctgctggc gcagatccag
     2941 caagcgctgg catcgttttg gaggctgaaa gcctgggccg gcgctttcat gagggcccgc
     3001 tcgacgtgac ggtgctgcag ggcgtcgatc tgcgcgtgcg cgccggtgaa acgctggcca
     3061 tcgtgggcca gtccggctcc ggcaagagca ccctgctgca cctgctgggc gggctggacg
     3121 cccccagcag cgggcgcgtg cggctgctgg ggcaggacct cggcagcctg tcgccggcgg
     3181 cgcagggcga gctgcgcaac cgccacctgg gcttcgtcta ccagtttcac cacctgctgc
     3241 ccgaattcag cgcgctcgaa aacgtcgcca tgccgctgtg gatccgccgc cagccgcgcg
     3301 ccgaggcggc cgaggcggca cgccacatgc tggcgcgcgt gggtctgcag gagcgcgtgc
     3361 ggcaccgtcc gtccgagctg tcgggcggcg agcgccagcg cgtggccatc gcgcgcgccc
     3421 tcgtcacccg cccggcctgc gtgctggccg acgagcccac cggcaacctc gaccgcgcca
     3481 ccgccgacgc ggtgtttgcg ctgatgatgg agctggcggc ccagcacggc acggccttca
     3541 tcgtggtcac gcacgacgcc cagctggcgg ggcgctgcgc cagggtgctg cggctggtgc
     3601 agggccggct cgggccggtg tgacctgtgc ccccttgggc ggccgtggcc tggtggccga
     3661 gggagcggcc gacccggcag cctgccggcg ccacccatgc gcgccaccgc ctgggccgga
     3721 tttatgattg tcgacaatca tgaaacaagg cgtacccgat ggcgagcgtg gagtctgacc
     3781 aggaagtgtt cgcgcgctgg gagcgcctga ttcagttccg gctgctggcc gacagccagg
     3841 agcccacccg caccgtgccc gagcaggtgg ccgcgcaggt gggcgaccgc atcatcggtg
     3901 gcgcgctggc gccgggcgcg cgcatcggcg agcaggaact ggccgacgaa ttcgccatca
     3961 gccgcgggcc ggtgcgcgaa gccatccgca tcctggagcg cgaaggattg gtgcaggtgc
     4021 tgccgcgccg cggcgcggtg gtgaccagcc tgtcggcaca ggagctgcgc gagctgttcg
     4081 agatccgtgc cggcctggtc gagatcgcgt tgcgcaaggt gatgggcgcg ggcgtgccgc
     4141 ccgagctgct ggccatcacg cgcgccggcg tggcccggct cgaggcgctg gccgaactgc
     4201 cggagggcgg cgatgcctat gccgagacga cctaccggtt gatccagatc ggagcgcatt
     4261 tcgccggcaa cgagcgcctg cgccgcatgg tggtggcgct gtcgctgcag agcctgcgct
     4321 acagcaagct gggcctggcc tcggtggcgc ggcgtcagcg ctcggtgaag ctgtggcgcc
     4381 aggcgctgca ggcgctggag cgcggcgacg tggaacggac cctgtggctg gcgcgccagc
     4441 gcagtcagga gtcggccgat gaagccgcgc gccggctggc cgagccgtcg ggcggcagct
     4501 gagcttcagc cggcctgttc tagtccactg aatcactgaa atcggatcgg cgctgtccgc
     4561 ccgatacggg cacgctgcgg cgttgcccat gctcgccaca gcacgggctg tggctgcgct
     4621 ttgcgccttg catcgcctcc cgtctcgggt gtccctcacc gcccatttca acctagaaac
     4681 ggcagttgac cgctggtcaa cttcgggcag accgcatcga tgcggggtct ggcggctgcg
     4741 atggccttca cctcctgggt gagcgcgctg cgcacccacc cgcaccgcgc tcgccgcgcc
     4801 atgcggcgtt gctcctgctt gcgggcagga gcgcgcagcg gcgtcgcgtc ttgcctggca
     4861 caccgatcac ggcacgggcg ggcgcgtgca acggccgttt ctgggaggct gtcgggcttg
     4921 gagcgccgaa accgttctcg aagagcggcg tagccaaaat cggctccagc gagaccagtt
     4981 tttgccggat ttgcagccca atagccgggc tcttgggcaa aaaatccggt gaaaaatggg
     5041 ccgctgcggt cgatttgcag ccggcgattt ccaagtccga cagtcttcta ggttaacgct
     5101 atgcagggcg accccttgcc tagcgtttac aggccctggc tggtgaagcg ttcgcgcaag
     5161 gcgggcttct gcaccttgcc ggccaggttg cgcggcagcg cctcgacgaa ggcgatgcgc
     5221 cgcggtttct tgtagccggc gatgcgctcg ccgcagtggg ccatcagcgc ctcggcgctg
     5281 gcggtgcagt cggcacgcag caccaccgcg gccgcgacca gctcgcccca ctgcgggtcg
     5341 ggcaggccca gcacggcggc ctccagcacg gcggggtggg tgctcagggc atcctccacc
     5401 tcgcgtgcgt agacgttgaa tccgccgctg atgatcatgt ccttgttgcg gccgacgatg
     5461 tgcagaaagc cctgtcggtc caaccgcccg aggtcgccgc tgtagtacca gccatcgcgc
     5521 acggtctcgg ccagcagctc gggacggcgc cagtactgca ccttgccgat gccttcgtgg
     5581 gcaatggtga tttcgcccac ctcgccgggc gcgacctcgc gcccctgggt gtccctgagc
     5641 acgatgcgca cgttggccgt gggtcgcccg caggagccga tcggttcgtc cgcgccgtcg
     5701 gcgctggtgc cctggtgctc atgcggatag agcacgcaca gcggctgcac cgcctcggtc
     5761 atgccgtact gctggcgcag gatcgggccg aagatcgcct gggcgcgccg gatcagcggc
     5821 accggggtgg gcgccgtgcc gtagtgaatg cgccgcagcg aggaccagtc accgcgcgcg
     5881 ccgccttcga tggcctccac cagccggtgc atcatggtcg gcaccagcat caggtgcgtg
     5941 atgcgctcgc gctcgatggt ggccagcagc gtggcggggt cgaactgatc cagcaccacg
     6001 ttgcgcgcac cgcgcagaaa acagggaaac aggtacaccc cggcggcgtg ggtcagcggt
     6061 ccgacgtgca gcatggcgtc gtccaccgac agaccgtatt ccatggccag aaagtggttg
     6121 gccaggcgcg cctgccagcg ctccagcgtg taggccacac ccttgggccg accggtggta
     6181 ccactggtgt aggcgatgcg catcaggtgg tcgggtccga tctcgatggc cggcgggtga
     6241 tccggtgccg ccgcggtggc cagccgccag tcggtcactc cgggggcgtc cacaccgaga
     6301 ccgagcacgt ggcgcagttc gggcagctgg tggccggcgg cctgggcgaa ggccgccgcc
     6361 agctcgccgt ggcgtggccc gaccagcacc gcgctggcct gtgcatcggc cagaatgtcc
     6421 agctgttcga ccagggtgtg gcgggcattc agggccacgt aggtgaggcc ggccttttcg
     6481 gcgccaaagc cgctgtcgag gctctcgacc gagttgtcca gcaacaccgc cagccgctcg
     6541 ccgggttgca ggcccagggc cagcgccgcc tgcgcgaaac ggttgctccg ggcctcgatc
     6601 tcggcgtggc tgtagcgttg ttcaccgaac accaccgcgg tgcgcgcgcc gtgctgcgcg
     6661 gcggcccagg tggtgagtcg tccggcgatc attccagcac cacgaccgtc tggccctcgg
     6721 ccacgctctc gccctcggcc accagcagct ccagcaccac accggcgcgg ggggcggtga
     6781 gcgggatttc catcttcatg ctctccagga tgaccagggt ctggtcggcg ctcaccgggt
     6841 caccggcgct cacctcgact ttccagacgg tcccggtgac ttcggacggc agatggatgc
     6901 gcggcatggg attctcctga atgtgggtgc ccgcgatgat agacaacatc aagattgttg
     6961 acaatcgcta tgttgggtga ttcaatcggc accgacgttg ccgccttgcc cctgtcctgg
     7021 cgcggccacg ccgtcccatc gaagaggaga tgtcatgcaa tttcgattcc tggccggtct
     7081 gatgctcggc ggtctgtgcc tggccgccac ggccctggcc caaacggcat ggaagcccag
     7141 cgaacgcatc aactacatca ttggcgtggc accgggcggc acggtggatc tgtatgcgcg
     7201 tgccatcaag gacagcctgg agcgcctgaa actgaccggc ggcgaacccg tgatcgtgga
     7261 gaacaaggtg ggtgccggcg gcgccctggc gatgaacgcg ctgcgcgccg gccacggcaa
     7321 cgcccattac ctgggcacgt tccataccgg cgccatagcc ggcgcggtga ccgggttgat
     7381 caaggccgat ccgcgtgaat tcatgccggt ggccatgctg gtggaagaga cctcggtggt
     7441 ggcggtgcgc gccgattcgc cgttgcgcag cggcaccgat ctggtggccg cgctcaaggc
     7501 cgacccgacg gcactgcgca tcgcggtggc gccggcgctg gggcagaaca tccacatggc
     7561 gatcgccaag ccgctgcatg tggcgggggt ggcgatcgac aagctgacca tcgcgccgtt
     7621 tcgctcatcc ggtgagtccg tgacggcgct gatcggcggt cacgtggacg tggtgtcggc
     7681 cactgcgccg gtggtgctgc cgcaggtggc caacggcaag ctgcgcatcc tggccagcgc
     7741 ggcgcccgtg ccgggcagcg ggccgctggc gggggtgaag acctggcgtg aacagggcgt
     7801 gccggccgac tacgtgagct acaacggggt cacgctgccg ccaggcgtcc atgccgaaca
     7861 gatccggttc tgggaggagg cgttgcgcaa ggtctcgcag gacccgcagt ggcgcgcgct
     7921 ggtcgaaaaa tcgggcaacc agccgatctt ccgcggctat gtcgaatccc accgctacat
     7981 gatgagcgaa tgggacgcgg ccgccgaact ggcccgcacg ctgggcctga agggcggccg
     8041 atgagcgcgc aacaaggcca gacgctggcg ccggccgggg aatgggcggc cgaactggcc
     8101 gagctgcagc agcgccgcgc cagcgcgctg gccatggggg gggcgcaggc gctggcacgc
     8161 atgcgcgaca gcggccgcat gaacgcgcgc gaacgcatcg ctgccctgat cgaccagggc
     8221 agttttcacg aaatgggcac cctggccggc aagggccact atgacgcgca agggcgctac
     8281 agccataccg acccgaccaa cgccatcgtc ggcaccgggc gcatcgacgg gcgcaaggtg
     8341 gcgctgcacg tggacgacta cagcatccgt gccggcagct ccgagggcac catcgccgac
     8401 aagtggatct acatcgagcg cctggcgcac cagctgcgca tgccgctggt gcggctggtc
     8461 gattcggccg gcggcagcgt caagctgctg atgcagctgg gcggcaccaa gatccccgaa
     8521 tacaccacct ggccggcgca ggagctgctg cagaccgtgc cggtggttgg cgtggcgctg
     8581 ggcgcctgcg ccgggcaggg ggcggtcaag gtgctgtctt cgcatttttc ggtgctggtg
     8641 cgcgggcagg cccaggtcat ggccgccggg ccccatgtgg tgcggcaggc ctacggggtc
     8701 gaggtcgaca agaacgagct gggtggccac ctggcgcagc gcaagagcgc cctggtgcac
     8761 aacgaggccg aggacgaggc cgacgcgctg gcgcaggtgc ggcgctttct gtcctatctg
     8821 ccgcgcagcg tgcatgagtt ggcgccggtg gtcgcctgcg acgacgatcc gcaccgcgcg
     8881 gacgactggc tcaaggacgc cgtcccacgc gaccggcgca agatctacga cccacgccgc
     8941 attctggcgg cggtgttcga tcagggcagc ctgttcgaga tcgggcgctg gcagggcggc
     9001 tcgctgctca ccctgctggg gcgtctgaac ggcgtgccgg tgggcgtgat cgccaacgac
     9061 cccaaggtgc agggcggcgc catgacgctg caggcggcct acaagatgga acgccacacg
     9121 cggctgtgca gccagttcgg gctgccggtg gtcaatttcg tcgatcagcc cggcaacgcc
     9181 accggtctgg aggccgagct cgccggcacc ctgctgggtg cggtgcggct gggcgaagcc
     9241 ctggcgcatt gccgctcacc ctgggtgtcg atcctgatgc gccgctgctt cggcatggcc
     9301 ggtgcgttgc atgggcccaa gggcggcgcg cggctgaatc accgctttgc ctggccgtcg
     9361 gcgcgctggg gctcgatccc gatcgagggc ggcgtgatgg ccgcgcacaa ggccgagatc
     9421 gaagccgcgc ccgacccggc cgccaggcgc gccgaactgg aggcgcacta tctggccatg
     9481 acctcgccgt ttcgcaccgc cgagaagttc ggcgtgctcg acatcatcga cccgcgtgag
     9541 acgcggcgcg tgctgtgcga ttgggtcgag gatgcgtggt acgtggtgcg aggtgacttg
     9601 gcgcagcgca gcgcaggtgc atgaggggca gcgatcgcta tataaacgat agctgctggc
     9661 gcatgttggg cgggcgctga ggcccaaacg gtcatgaaat ccgtcagagc acgcggccga
     9721 accacagcag cgacaacccg cccgccaaca gcgccagcag cgcgccggcg agggcgaaca
     9781 gcgacgacac ctcggtttcc ttcttctcca gggtcaggcg gctcgacagg ctctggtaga
     9841 ccttcttcag gtcctgcgcg gtgccggcgt agaagtactc ggcggcggtc ttctgggcga
     9901 tggtcttgag ggtttcctcg tccagccgca cgcgcatcga ccagccttcg aagccgatga
     9961 tctcgccatc gaccgtgccg atgcccacgg tgtagacctt gacgccgcgc tcggcggcca
    10021 tcttggcggc gtccagcgga tcgacgccgg tggtgcgctg gccatcggtc agcatgatga
    10081 tggcggccga gctgtaggag cccggcggca ccggcacgaa ctcgttctgc ttgggtggcg
    10141 gttgatccag cgagcgaccc cagccgccgc gcatgcggtc ggggctgagc tgctgcagct
    10201 cgatgccggc gtggggaaac agcgtggcca gcgaaatcac gatggcgttg ccggtggcgg
    10261 tggcgcgctg catctggaag cgctcgatgg cggtgaccag gtcctcgcgg ttgagcgtgg
    10321 gctgctgcgc cacctgggcc gagccggcaa aggccacgat gcctaccttg acgtcgcgcg
    10381 gcagctcctt caggaaggtc ttggctgcct cctgcgccgc caccaggcgg ttgggctcga
    10441 cgtcggtggc gcgcatgctg gccgacacgt ccatcgccag gatgatggtc tgctggttgg
    10501 agggcagggc gatcaccgcc accggccgcg cgctggccag cagcagggcc gccagcgcca
    10561 gcagcagcag cagtggcggc aggtggcggc gccagccggg gccgcggccc tgcgcctgcc
    10621 gcacgatcga caggctggcg tagcgcaacg ccagcttcct gcgccggcgc agcagccagg
    10681 cgtacagcag caccagcagc ggcagcagcg ccagcagcca cagcatgtgc ggccacagga
    10741 aggcgggctg cgggatcgcc gcaggccact tcaggaaggc gggccaggcg aagttcatga
    10801 gaccttcatt ttgatccgat cagccggcct tgcgaaggtg cgccggcagc ccctcgccgg
    10861 cggccaggcg ggcgcgctgg cggcgcagct cgacgaagcg cacgatggcg tcggtcagcg
    10921 caccctcggt ggacagctcc agcgcgtcga cgccggcgcg cgccagcgtc tggcgcagct
    10981 cgtcctcgtg gcgggccgcc agggcggcaa agcgctgccg aaagccggcg tcgtgcgtgt
    11041 ccaccagcat ctgctcgccg ctctcggcgt cgcgcagcgt catcaggccg aggtcgggca
    11101 gcgcacgttc gagcggatcg agcaggcgca ccgccaccac gtcgtggcgg ctggccagtt
    11161 gcgccagcgg ctgttcccag ccgggcgcgc tgatgaagtc cgacaccacg aacaaggtgg
    11221 cgcgccggtg caccaggtgg ccggccgcgc gcagcagctc ggccaggcgc gtggtccccg
    11281 cctcggccgt cggggccggc gccggccggt ggccgatggc gtgcagcaga tgcagcacat
    11341 ggcggcggct ggcacgcgcc gggatcacct gctcgacccg cgcgccatag agcagggcgc
    11401 cgacgcggtt gccgtggcgc gtcagcaggc gcgccagcac ggcgacgaat tcggccagca
    11461 cctcgcgctt gagccgctgg ccggaaccga aatcgaccga ggggctgagg tccagcagga
    11521 accaggccgc catctcgcgg tcttcggtgt agacgcgcac atgcggggtc tgcaggcgcg
    11581 ccgtgacgtt ccagtcgatg tggcgcacgt cgtcgtgggc ctggtattcg cgcaggtcgg
    11641 ccagatccag cccgctgccg cgcatcaggg tgcgcacgtc gccctgcagc acgccgtcca
    11701 ggcgcctgag caccgtccat tcgagccggc gcagcagggc ctcggtgtcg gccgcggccg
    11761 gcccgggctg cgggtcggcg cgcgccgggt tgggcaccgc gtgcggcggt ggccggcgtg
    11821 ggcgcagcca gcgcagcatc agcggcctcg tggcgcagcg cggtggtcca tgctcaggcg
    11881 gccagcggct cgtgcgccag cggcttggcc ggcggcgcca ccttggccat caggcgcagc
    11941 acgatctggt cggccgacaa gccctccgac agcgcctcgt aggacagcac cagccggtgg
    12001 cgcagcacgt ccggcaccag gtcgctcatg tcttcgggca gggcgtaggc gcgcccgcgc
    12061 atcagcgcca gcgccctggc gccttcgatc aggccgatgg tggcgcgcgg gctggcgccg
    12121 tagccgagca tgggcgccag ctccttgagc ccgacgtcgg ccggccggcg cgtggccgcc
    12181 accaggcgga cggcgtactg cgtgagcgag gggtcgacgt acacctgccg gcattgctgc
    12241 tgcagggcgg ccagctgctc catgctggcc accgcctgca cctcgatgcg cgggccggtc
    12301 acgcgctggg cgatcacgaa ctcctcctcg tcgctcgggt agtccaccag caccttcatc
    12361 atgaagcggt cgacctgcgc ctcgggcagc gggtaggtgc cctcggtctc gatcgggttc
    12421 tgcgtcgcca tcaccaggaa cggctcgggc acgaagtggg tctggccggc aatggtgacc
    12481 tggcgctcct gcatcacctc cagcagcgcg ctctgcacct tggccggcgc gcggttgatc
    12541 tcgtcggcca gcagcaggtg ggtgaacacc gggcccagcg cggtgctgaa ctcgcccgtc
    12601 ttctggttgt agatgcgggt gccgatcagg tcggccggca ccaggtcggg cgtgaactgg
    12661 atgcgccgga actggccctg cagcacggcc gccagcgtct tgacggtgag cgtcttggcc
    12721 agccccggca cgccctcgac cagcaggtgg ccgccggcca gcagggccac catcacgcgc
    12781 tccaggaagc ggtcctggcc gaccaccacg cgcttgacct cgtagaggat gcgctccatc
    12841 agctcggccg tggccgggtc ggtctccaaa cgcgggttgt ccttgtccgt catgcgcggc
    12901 atgctcctga aaacggggtt agaagggaat ctgcccggcc ccggcggcgg cgttttcgat
    12961 cggcaccgca aagccgatgc ccaggaaggt gcgcgccggc gtggggttga ggatggcggt
    13021 gacgatgccc accacctggc cgtccatggt caccagcggc ccgcccgagt tgcccgggtt
    13081 ggcggcggcg tcgaactgga tcaggttgcg catcaactgg ccgccgtcgg gcgagcgaaa
    13141 gccgcgcccc aggcccgaca ccacgccggc cgacaccgag ggcccgatgc cgaacgggta
    13201 gccgatcgcc accacctcgt cgccgggcgc cagatcgccg gtggagcgca tggtggcggc
    13261 ctgcaggtcg tcgggcagct tgtgcgcgcg cagcaccgcc aggtcgttct cgggctgcat
    13321 gttgacgatg ccggcctcgc tctccaggcc gtcggcgaag gtcacggtga tgcgctcggc
    13381 gccctgcacc acgtgcaggt tggtcaggat ggtgccgttg tcgatgatca ccacgccggt
    13441 gccgatgccg cggcccagtt caggctggcc tttgcggccc ttcttctgtt cgaccacgcc
    13501 ttccacccgc accagcgaag gtgccaccgc ggccgcagcg cgcgccgccg ccgagggcag
    13561 taccttgtgc gtggcggtgt gcagcacggc gtcgtcgatg tccttttgcg tcagctggcg
    13621 cggcgccggg cgccgccagg cgcccaggtc gaggcccagc gccagcgcca gcgccagcgc
    13681 caggaccgcc gcccacagcg cgccggggtg gcgcagcgcc gcccccaggt gtgggcgccg
    13741 gcgcgcacgc tcgggcgcca gcgccggcgc cggcgccggg gcatcgggcg ccaccactgg
    13801 ggcaggggac gatcggctgt acagcgcgct gcgacgcatg ggctgacccc gggctggtga
    13861 atgacgaagc tcacggtacc atgattcggc acgccccggt agcggggtgg tcatggaggt
    13921 ggcatgggcg cgatgacggt gttgcgggcg ctgctggtgt gggtcctgct gctgttgctg
    13981 gcgatcgcca acggcgtgct gcgcgaggcc gtgctgatcc cgcgcctggg cgggccatcc
    14041 ggcctggtgc tgagcggcct gctgctgtcg gcgctgatcc tggtgctcgc ctggtggctg
    14101 ctgccctgga tcggtgtgcg cggccacggg ccgctgctgc tgaccggcct gggctggctg
    14161 gcgctgaccc tgggcttcga gttcaccttt ggtctgtggc gcggccgcac gctggccgaa
    14221 ctgctggccg cctatcggtt cgagggcggc aacctgtggt cgctggtgtt gttggtgacg
    14281 gccggcgcgc cgtggctggc cggccggctg cggcgctgta cctgacggtc gcggctgcgg
    14341 catgatgcgc gcatggccgc ctggatcgac acccactgcc acctggacgc cccggagttt
    14401 tcggctgatg tggccgccgt gcgtgcgcga gcagctaccg aaaacgtagc gatctgcgtg
    14461 atcccggcgg tcgagccggc ggccttcgag gcggtgcgcg cgctggcgca ccgctttggc
    14521 gatgcctacg cgctgggcat ccacccgatg tacgtggcgc gcgccccgga cgacgcgctg
    14581 gcgcggctgg aggcggcgct ggcgcgccat gccgacgacc cgcgcctggt ggcggtgggc
    14641 gagatcgggc tggatctgtt cgtgcccgaa ctgggcgtgg cgccgctgcg tgcgcgccag
    14701 cagcatttct tcatcgagca gctgcggctg gcgcggcgcc acgacctgcc ggtgctgctg
    14761 cacctgcggc gcgcggtcga cgcggtgttg caggtcctgc gcgaggtcgg cgtggccggc
    14821 ggcatcgccc atgccttcag cggcagccgc cagcaggccg atcagttgct ggcgctgggc
    14881 ttcaggctgg gctttggcgg tgcggtgacc tacccgcgcg ccagccgcct gcgcgcgctg
    14941 gcggccgagc tgccggccag cgccatcgtg atggaaaccg acgcgcccga catcccgccg
    15001 cactggctct acaccaccgc cgaagcgcgc gcgcagggcc agccccaggg ccgcaacgag
    15061 ccgggcgagt tgccgcgcat cggcgccgag ctggcggcgc tgcgtggcgt ctcggcggcc
    15121 gactgggccg acctcagcac cgccaacgcg ctggcggcgc tgccgcgtct gaggcgactg
    15181 tggggggccg gggagcacga tcgatgacga tgacccatga ggcagggcga aggacctcgc
    15241 tgcgcttgga cggcgggggc gcctcggggc atgggggcgc cgtgggcgct cgttgaacgc
    15301 gcagcgccgt cggcctgcgg gggcgacgtg gcacacccac cacgtcaagg ctatcgtttt
    15361 catagctgct tgcgcagtct ggacaagctt cagaggcatt ttttgcatgg aatccggcgg
    15421 cgctggcgcg cgctggcgcg cgctggcgcg ggcgcggcgg ggtcggtggg gctttgcata
    15481 tcttcacctg gcgctgacgg gcggcccggg gccgggccgc tacgatgcgc gctgaagcgc
    15541 cggcgcctgt ccctgcgcgc gcgcggcccg ccatgcattg cccaccatga ccaccaccgt
    15601 cgacacaccc gcaggctcac ccgccgccac cccgaccgcc gacgccgcac cgcgccgccg
    15661 cggcctgggc gtcacgctca cgctgctgct gatcgccgcg ctggtgggca gcgcctggtg
    15721 gctggtgcag cgcgcgcgcg cgcccgaggc ggcggccccc gcggcagcgc cgggcggcgc
    15781 cggtggcccg gggccgggca gcgccagcgt caccgtgggc gcggcgcacg cccagcgcgg
    15841 cgaactgccg atcgtcatcg acgccctggg cacggtgacg ccgccggtca ccgccaccct
    15901 ggtgccgcag gtgtcgggcg tgctgaccga ggtgctgttc accgaggggc agatggtgag
    15961 caagggccag gtgctggcgc gcatcgacgc ccgaccctat gaacaggcgc tggcgcaggc
    16021 gcgcggccag cgcgccaagg acgaagcgca gctggccgcc gccagggtga cgctggcgcg
    16081 ctaccagacg ctgtggcagc aggactcgat cgcgcgccag gacgtcgaca cccaggcggc
    16141 gctggtcaag cagctcgaag gcgtggtcga ggccgaccgc gccagcgagc gcgcggccca
    16201 gctcaatgtc ggctttgcca gcatccgcgc gccgatcggc gggcgcatcg gcctgcgcgc
    16261 catcgacccc ggcaacctgg tcagcaccgg cagcgccggc ggcattgcca ccatcacgca
    16321 gatcgcgcct atcgacgtgg tgttctcggt gccgcaggac cgcatcgccg ccgtgctggc
    16381 ggcccagcgg cgcgggcgcc tgccggtgca ggcgttcgac cgcgcgcgca gccagccgct
    16441 ggccgaaggc agctttctga cgctggacaa ccaggtcagc accgccaccg gtacggtgcg
    16501 tgccaaggcc cgctttgcca acgccgacgg cgcgctgttt cccaaccagt tcgtcaacgc
    16561 ccggctgcag ctggggcacg acagcggcgt gctggtgccg gtgacggcgg tgcgcaccgg
    16621 cccgcagggt gactacgtgt acgtgatcga cgacgagcgg gtggcgcgca tgcgcccggt
    16681 caggcgcggc atggcgacgg tcgatcaggt gctgatcgtc cagggcctgc aggccggcga
    16741 gcgcgtggtg accgaaggcg gcgaccgcgt caaggacggc ggcaagctgc gcctggcggg
    16801 cgaatcgccg ggcccgggcg gcgccgccgg catgcgcccc ggtgcggcgc cagcgcccgc
    16861 cccgacggcg ccggcagcta tcgcatcagg agcaggtgag gccggcgcgg ctgccccgcc
    16921 gtggctggag cgcctgccgc cggagctgcg cgacaaggtg cgcgccatgt cgcccgagga
    16981 gcgccgggcc ttcctgcagc agcgccgcgc ggcggccagc gcggccgatt cgggccgctg
    17041 aggcggcggc agcaccggcc cctcgtcatg aacgtctcgc gcctgttcat ccagcgcccg
    17101 gtggcgacgg cgctgtgcat gctggccatc gtgctggccg gcctgctggg cctgcggctg
    17161 ctgccggtgg cggcgctgcc gcaggtcgac tacccgacca tccaggtgca gacgctctac
    17221 cccggcggca gccccgacgt gatgagccgc accgtgacgg cgccgctgga gcgtcagctg
    17281 ggccagatgt ccgggctgga acgcatgagc agcgtcagca gcgccggcgt gtcgctgatc
    17341 acgctgcagt tcgcgctgca cctgaacatg gacgccgccg agcagcaggt gcaggcggcc
    17401 atgaacgccg ccgccacgct gctgccggcc gacctgccca cgccgccggt gtacgccaag
    17461 gtgaacccgg cggacgcgcc gatcctgcag ctggccgtca cctccgacag cctgccgctg
    17521 cccgaggtgc agaacatggt caacacccgg ctggcgctga agatcagcca gatcaacggc
    17581 gtcggcctgg tgacgctggc cggcggccaa cgccccgccg tgcgggtgca ggccaacctg
    17641 caggcgctgg cggccatggg cctggggctg gacgccatca gcagcgccat cagcgctggc
    17701 aacagcagct cggccaaggg cagcttcgac ggcccgacgc gccagtacac catcaacgcc
    17761 aacgaccagc tgctgacggc gcacgaatac cgcgagctga tcgtcgcctt cgtggccggc
    17821 cagccggtgc gcctgaagga cgtcgccgag gtggtggacg gcgccgaaaa ccgtcgcctg
    17881 ggggcctggg tggctatcaa aaacgatagc gcctcgcgca cgccggatgc gcgccagagc
    17941 cccggaacat ctgaaatcga gctgccgctc acgccggcca tcgtgctcaa cgtgcagcgc
    18001 cagccgggcg ccaacgtgat cgccaccgtc gacgccatcc ggcgccagct gcccgagctg
    18061 cggcaaagcc tgcccgacag cgtgcggctg accgtgctga gcgaccgcac gcagggcatc
    18121 cgcgcctcgg tggcgcacgt ggagctggag ctgggcctgg cggtggtgat ggtggtgctg
    18181 gtgatctttg cctttctgca cagcgcgcgc gccacgctga tcgccagcat cgcggtgccg
    18241 atctcgctgg ccggcacgct ggcggccatg gtgctgctgg gctattcgct caacaacctg
    18301 agcctgatgg cgttgaccat cgccaccggc ttcgtggtgg acgacgccat cgtgatgatc
    18361 gagaacatct cgcgccaccg cgagctgggc aagccgccgc tgcaggcggc gctggcgggg
    18421 gcgggcgaga tcggcttcac catcatctcg ctgacggtgt cgctgatcgc ggtgctgatc
    18481 ccgctgctgt tcatgcagga ggtgatcggc cggctgttcc gtgaattcgc cgtcaccctg
    18541 gccatcacca tcctgttttc cgcgctggtg tcgctgaccc tggtgcccat gatgtcggcg
    18601 cgctggctgg agccgctggg tcaggcccat ggcccggtgg gcgcccgcat gcaggcgtgg
    18661 ctggacgcca ccatcgccgg ctacgaccgc gcgctgcgct gggtgctggc gcgtcagccg
    18721 ctgacgctgg cgctggcgct gctgacgctg gccgtcaccg tgctgctgta cgcgggcatg
    18781 cccaaggggc tgttcccgac ccagagcacc ggtcagctgc agggcctgct gcaggcggcg
    18841 ccggaggtgt cgttcgagcg catgtcggcg ctgcagggtg cggcggcgca ggtggtgctg
    18901 cgcgatccgg cggtgcgctc ggtcagttcg gtggtggggg tggacgcggc caacaacagc
    18961 gcgctggcga gcgggcggct ggtcatcaac ctgcggccgc gcacgccctg gggcgacacg
    19021 caggcgaccg tcatgcagcg gctgcgccgg caggtgggcg agcaggtggc cggcgtcacc
    19081 ctgtacctgc agccgacgca ggacctgacc atcgacgccg tcagcggccc gacggcgcag
    19141 cgcttcgacc tggagggggt ggacacggcg ctggtcgatg gctgggcgcg gcgcctggtg
    19201 gcgcgcctgc agcgcctgcc cgagctgcgc cacgtcacca ccgacgccgg cgccaccggc
    19261 ctggccgcca tggtgcgggt cgagcgcgac accgccgcac gcctgggcgt gacagccagc
    19321 aacctggaca acgcgctcta caacgccttt ggccagcgca tcgtctccac catcttcagc
    19381 gaaaccaacc agtaccgcgt gatcctggag gccgcgcgcg ccgtggacgc cagcctgcgc
    19441 accctgcgcg agctgccggt gcgcaccagc gtcggcggca ccacgccgct ggaagccttt
    19501 gcgcgcgtgg tggaagagcc gtcgccgctg caggtgcagc gggtcgacca gtaccccgcc
    19561 gccaccgtgg gcttcgacac cgcgccgggc gtcagcctgg gggcggcggt gagcgccatc
    19621 gaggcggcgg tgcgcgccga gggcctgccg gcggcgctga cgctgcgcat gaccggtgcc
    19681 gccggcgcct tccagcagtc gctgggcaac cagctgtggc tgatcctggc ggcggtggtg
    19741 tgcgtctaca tcgtgctggg cgtgctgtac gagagctacg tgcacccgct gacgattctg
    19801 tcgacgctgc cctcggccgg cgtgggcgcg ctgctggcgc tgtggctgac cggccatccg
    19861 ctggacgtgg tgggcatcat cggcctggtg ctgctgatcg gcatcgtgaa gaagaacgcg
    19921 atcatgatga tcgacttcgc catcgaggcc gaacgcgccg gcaagtcggc cctggaggcg
    19981 attcaccagg cggcgctgct gcgtttccgg ccgatcctga tgaccaccct ggcggcgctg
    20041 gccgccgccg tgccgctgat gctgagctgg ggcgacggcg gcgagctgcg ccggccgctg
    20101 ggcatcgcca tctttggcgg tctggtgctg tcgcagctgc tgacgctgtt caccacgccg
    20161 gtgatctacc tgtggttcga ccgcctgggc cagcggctgg ccggtcgcgc cccggtgggg
    20221 gaggtggggc atgccgactg aggcgctgca cggcgggcca agcgctactg ctttggtagc
    20281 tggtggcgct tgctgggcgg gcgttgcggg ctttttttgt cgcttgtggc tggcgccctc
    20341 gaccgtctgg ggagaggccc gggtccgcat gcccccggcg gggcgctgag ccatgaacct
    20401 ctcgcgcccc ttcatccacc ggcccatcgc caccgtgctg ctcaccctcg ggctggcgct
    20461 ggccggcatg gcggcgtact tcgtgctgcc ggtggcgcgg ttgccggcgg tggagtttcc
    20521 ggtcatcttc gtcaacgcca gcatggccgg cgccagcccg gccgacatgg cgcgcaccgt
    20581 cgccacgccg ctggaacgca ccctgggcac catctcgggc gtcaatgaaa tgacctccag
    20641 cagcaacacc ggctcgaccc gcgtggtgct gcagttcgat ctggggcgcg acatcgacag
    20701 cgccgcgcgc gacgtgcagg cggccatcaa cgccgcccgc atcgacctgc cggccagcct
    20761 gcgcagcaac ccgacctacc gcaaggccaa tccggcggcg cagcctttca tgacgctggc
    20821 gctgacttcg accacgcgca cgccggggca aatctacgac gccgtcagca acatcgtcag
    20881 ccagcgcctg cagcaggtcg atggcgtggg cgacgtggag ctgggcggcg gctcgctgcc
    20941 ggcggtgcgg gtgcagctca accccttcgc gctgcacgag ctgggcatcg gcgccgagga
    21001 cgtgcgcgcc gccatccagg cgcacaacgc caaccgcccc aagggcgtga tcgaagtcgg
    21061 cggcagcgcc gacgggcgcg cgctgcaggt gctgaccccc gcgcccggcc tgcgcgcggc
    21121 cgattaccag gacctgatcg tggccctgcg cgacggccac gaggtgcgcc tgtcgcaggt
    21181 ggcgcgcgtg atcgacagcg tgcaggacac gcgcacgcgc ggcatgttca acggccagcc
    21241 ggcgatcgtg gtcaacatca cgcagcagcc caccgccaac ctgatcgaga ccgccgacgc
    21301 catcgacgcg ctgctgccgc agctgcgcga gcagctgccc agcgacatcg agctgcacgt
    21361 ggcggtcgac cgcaccggct cggtgcgcgc ctcggtgcgc gaagtcgaga tcaccctggt
    21421 gatcgcggtg ctgctggtgg tgggtgtggt ggggctgttc ctgcgcacgc tgcgggcggc
    21481 gctgattccg gcggtggcca cggtggtgtc gctgctgggc acgctgggtg tgatgtacgt
    21541 gctgggcttc agcctgaaca acctgaccct gatggcgctg acggtggcca ccggcttcgt
    21601 ggtggacgac gccatcgtgg tgctggaaaa catcacccgc cacctggagc agggcgtggc
    21661 gcggcgccgc gcggcgctgc agggcgcgcg cgaggtcggc ttcacggtgc tgaccatcag
    21721 cgtgtcgctg gtggcggtgt tcattccgct gctgttcatg ggcgggcagg tggggcgcat
    21781 gttccgcgag ttcgccgtca cgctgtcggc ggcggtgctg atttcgctgc tgatctcgct
    21841 caccaccacg cccatgctgt gcgcgctgct gctgcgcccg ccggcggcgg gcacgccacc
    21901 gcgccagagc gtcgccggcc ggctgcgcca gcgcctgggc ggctgggcgc gcggcagcca
    21961 gcggtcgctg acgcgcgcct acgccggcgc gctggactgg gcgctggcct cgccctggct
    22021 ggtggtggcg gtgctgctgg tggtgatcgg gctgaacggc tacctgttct cggccatcac
    22081 caagggcttc ttcccggatc aagacaacgg cctgatgacc ggcgggctgc gtgccgacca
    22141 gagcatctcc tccggcgcca tggccgacaa gctgcagcag gcggtcgaca tcctgcgcgc
    22201 cgatccgtcg gtgggcacgg tggtggcgtt ttcgggcggc tggcgctcgg gcggcggctt
    22261 tctgtcggcg gcgctcaaac cacccggcca gcggccgccg ggcgagagca cgcgcgccgt
    22321 catccaacgg ctgcgcccgc agctggggcg catcaccggg ctgcgcgtgt ttctcaaccc
    22381 ggtgcaggag ctgcgcatcg gcgggcgcag cagcaacagc agctaccagt acacgctcaa
    22441 gaccgacaac caggccgacc tgcgaaactg gacgcgccgc ctgtccgagc agatgaagcg
    22501 cgacgcccgc ctgaccgaga tcgacgacga ccagagcgag cacagcgtgg agaccttcgt
    22561 gcagatcgac cgtgagcgcg ccgcccgcct gggcgtggcg ctgccgggca tcgacgccac
    22621 gctgtacgac gccttcggcc agcgccaggt ggccactatg ttcagcgcgc tcaaccagta
    22681 cgcggtggtg ctggaatggg cgccggagca tgcgcgcgcg ccgcaggcgc tgcacgacgt
    22741 gatgctgccg gtggcgccgg cggtgtcggc gcggctggcg ggccaggcgg cgccggtggc
    22801 cagcgccacc tcggccaacc cggcgcagcg cggggcctcc acagggcagc cgatttcgtt
    22861 cacgccggcc ggcatggtgc cgctgccggc gatcgccgag ttcagcgagc gcgccgtgcc
    22921 caccagcgtc tggcacgacg gtggcgagct gtccagcacg ctgtcgttcg acctcgccga
    22981 tggggtgtcg atcgagcagg gccgccaggc ggtgctggac gcggtcgatg ccgtcggcct
    23041 gcccaacaac gtgcgcggcg tcttcagcgg cgcggcggcg caggcgcagc agacgcaggc
    23101 gcagcaggtg ctgctgatcg gcgccgccat cgtggtgatc tacatcgtgc tgggcatcct
    23161 gtacgaaagc ctgatccacc ccatcaccgt gctgaccacg ctgcccagcg ccggcttcgg
    23221 cgccgtgctg gcgctgctgc tgctgaagat ggagttttcc atcatggcgc tga
//
LOCUS       DAOOOG010000021        22473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_2228_length_22473_cov_29.682666, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000021 DAOOOG010000000
VERSION     DAOOOG010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 22473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22473
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2228_length_22473_cov_29.682666"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PK242_03170"
     CDS             <1..1145
                     /locus_tag="PK242_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOZ93053.1"
                     /translation="GVGVMFYVLLALRAELRARGRFSAATQPRLDSLLPLVALGTVAD
                     VVPLDANNRRLVAQGLRRIRAGQMPVGLAALFAVAGRATPRAGSFDFGFALGPRINAA
                     GRLADMTLGIELLLTDDAARAAELAATLDAINRERREIEGGMREQALQIAEGLFEPHA
                     DPPAALAVFDPDFHEGVVGIVAARLKDRLHRPTFVFAASGAPGREHELKGSGRSIAGF
                     HLRDALDLVAKRHPGVLLRFGGHAMAAGCTIDEHQFDTFERALQQVATEWLDAATLTR
                     RLHTDGALPPEYRRPDVVDLLHDAVWGQGFAPPVFSEELEIVGQRLVGEKHLALKLRH
                     QGQPVDGIWFGRTEPLPARALLAFRLEADEWQGVRRVRLLIEGLGA"
     gene            1304..2077
                     /locus_tag="PK242_03175"
     CDS             1304..2077
                     /locus_tag="PK242_03175"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HOZ93054.1"
                     /translation="MAAFDDEKVLSVHHWTDRLFSFTTTRSQSLRFSNGHFTMIGLRV
                     NGKPLLRAYSIVSPNYEETLEFLSIKVPDGPLTSRLQHIQPGDSIIVGHKPTGTLLID
                     YLLPGKRLYLFGTGTGLAPFMSIIRDPDTYEKFDQVILVHGCRLVSELAYRDYITQEL
                     PRHEFLGELVSSQLRYYPTVTREPFPTQGRIPELIESGRMARDLGLPDLHPQLDRVML
                     CGSPEMLKSIKEVLEKRGFKEGNTTTPGDFVVERAFVEQ"
     gene            complement(2111..2956)
                     /locus_tag="PK242_03180"
     CDS             complement(2111..2956)
                     /locus_tag="PK242_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOZ93055.1"
                     /translation="MHQRKPLDRLLLATALLLAHWGVGAQDLQAQMAQPLELKPVAFS
                     DEIKDSIGLFTNVANTVFKPAGDGPFPATVLMHTCGGLKGPPNAHMKQHAQSLLAAGH
                     VVLVIDSFGPRGFDSCATRVLSHSAGIADAYAALALLAGKPFVDKSRIYQAGYSWGAF
                     VSTHLASPQSARLVGSELRFAATVANYGSCAFKQYQLVLSDTDRPLLMLMGGRDAEVP
                     ASACFPLLEQMKAAGKPVEWHLYPQATHAWDKPGLPARGYVYDAQVTQDATARMVAFF
                     AAGGR"
     gene            complement(3033..3533)
                     /gene="coaD"
                     /locus_tag="PK242_03185"
     CDS             complement(3033..3533)
                     /gene="coaD"
                     /locus_tag="PK242_03185"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOZ93056.1"
                     /translation="MSQPVIAVYPGTFDPITLGHEDIVRRAARLFERVVIAVATAHHK
                     KTLFTLDERLAITREALADLPQVSVVPFTGLVTAFAVAQGARVMVRGVRSVTDFDYEA
                     QLAGMNRALVPEVDTIYLTPSSATQHISSTLVREISMLGGDVAQFVSPPVLAHLMRRH
                     GERGRP"
     gene            complement(3587..4195)
                     /gene="rsmD"
                     /locus_tag="PK242_03190"
     CDS             complement(3587..4195)
                     /gene="rsmD"
                     /locus_tag="PK242_03190"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOZ93057.1"
                     /translation="MSQPSKPAARGRAALPRQVRVIGGQWRRHLLPVPDRPGLRPTPD
                     RVRETLFNWLGQTLTGWQVVDAFAGTGALGLEAASRGAAEVLLVERDDGLAAGLRALK
                     QRLGAAMVRVQAGDGVAALAALAPASVDLVLLDPPFDQPALLAPALTAAARAVRPGGF
                     VYLETPQRWDDAALDALGLALHRHLKAGAVHAHLLQRPAPSA"
     gene            4383..4928
                     /locus_tag="PK242_03195"
     CDS             4383..4928
                     /locus_tag="PK242_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOZ93058.1"
                     /translation="MSAKPQNPVDTDAPLTNFSHCHAGILRHLDDFGQLPALLEPAQR
                     LRQIAERFVEFFREAVFEHHQEEERELFPAVLASAAPGAERERVQQMVDQLTREHRDV
                     EAQWKRLEPQIKKLAKGQPADVDPQDVRSLVAQYQAHARFEEQEFLPLSEQILSRNSD
                     HMAALGLSLHMRHQPAFLSHI"
     gene            complement(5119..5940)
                     /locus_tag="PK242_03200"
     CDS             complement(5119..5940)
                     /locus_tag="PK242_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOZ93059.1"
                     /translation="MTTPIPRTPPPEGCIDTQVIDDQILLIGLNRPAKRNGWTPRMFR
                     ELAEAYTRLDDDPTLRVGVLHAFGEHFTAGLDLPLVVEHLQRGEPLTPYGLVEPHELG
                     LPGYRRRQKPMIAAVQGICFTVGIELMLAADIAIAADNCRFSQMEVQRAILPAGGATL
                     RMAERAGVGNAMLHLLTADQFDSAEALRCHFVQKVVPAAELLDSALTLARRIAAQAPQ
                     AVVATRLNVLKAIELGQAAAVADFGPVQRRLAASEDAAEGVRAFIEKRPARFTGR"
     gene            complement(5987..7351)
                     /locus_tag="PK242_03205"
     CDS             complement(5987..7351)
                     /locus_tag="PK242_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ93060.1"
                     /translation="MKTIAVRALLVCSGAFFHSVSAWAAIPIQHWTQPSGAQVWLVES
                     PAIPMLDVQLDFDAGSRRDPPAQAGLASATALLAGKGVAARGEQPALDENQLGEAWAD
                     LGASFGASAGADRMSFALRTLTDAELLRQAVQLAARELGEPAWPEPVWQRERARLSAA
                     IQEARTRPATQAALAFQQAVYGRHPYGQDSTPETLARIQVADLQAFYRQHVQPCRARV
                     SLVGAVDRAQADALVRELLGRLPSAGACAPLPPVAEVAPLQAAREIRIPFQSAQAHVL
                     IGQPGYKRSDPDFFALLVGNHILGGGGFTSRLMREVREQRGLSYSVYSDFAPGLHAGA
                     FSIGLQTRPDQADAAVAVVRQVLADFVAQGPSADELKAAQDNLIGGFALRLDGNRKLL
                     ANVANIAWNGLPLDYLEHWSARVAAVTLADIRAALQRKLQPERMVTVVVGAPEAGQRN
                     GNDR"
     gene            complement(7425..8852)
                     /locus_tag="PK242_03210"
     CDS             complement(7425..8852)
                     /locus_tag="PK242_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ93061.1"
                     /translation="MNFRPAFLLPVLLAALLGACASAPPPAATDAASPAAAAGAVQRF
                     TLDNGLTVIVKPDRRAPTAVHMLWLRVGAMDEVDGTSGVAHVLEHMMFKGSERLAPGE
                     FSRRVAQLGGRENAFTSLDYTGYYQQVPAARLGEVMALEAERFAHLRYSDEVFAKELE
                     VVKEERRLRTEDNPRALLFEQQRAVGFIASPYRRPVVGWMGDLQAMTADDARAFHRQW
                     YVPANAAVVVVGDVEPAAVLALAQRHYGGMAARPLPGRKPRPEPAQLGLRRIEVKAPA
                     EQAVVSLAFRVPRLDGLEPGADNDDALALTVLAAVLDGYSGARLERALTQGPDRVADS
                     ASAGNGLLGRGPQWFTLGGVPATGKSAAQVEAALRAEVARVAEQGVSPAELARVKTQW
                     VASEVYKRDSVMGQAQELGHLWVQGLPLDADQRLVARLRQVTAAQVQAVARKYFGDDQ
                     LTVATLVPQPRAAGATRPAPAPTRH"
     gene            8959..10035
                     /gene="selD"
                     /locus_tag="PK242_03215"
     CDS             8959..10035
                     /gene="selD"
                     /locus_tag="PK242_03215"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOZ93062.1"
                     /translation="MNSPAAAPATPTPEPRLTSLSHGGGCGCKIAPGVLSAILKDTLA
                     MPMPPELLVGIQTADDAAVYRLNDEQALVATTDFFMPIVDDPFDFGRIAAANALSDVY
                     AMGGTPIMALALVGMPINVLSLQTIGRILEGGASVCRDAGIPVAGGHTIDSVEPIYGL
                     VALGLVHPERVKTNSGAQVGDRLVLGKPIGVGVQSAALKKGALPAAGYAAMLASTTQL
                     NRAGPALARLPGVHAITDVTGFALAGHALELARGAGVTVHIDWPSVPLLPGVGQLAAQ
                     GMVTGASGRNWAAYGHDVALPAGFGDSQRALLTDPQTSGGLLVACDAASVDEVLATFR
                     HSGFAQAAVIGRIEPAGASPLVVA"
     gene            10282..11421
                     /locus_tag="PK242_03220"
     CDS             10282..11421
                     /locus_tag="PK242_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93063.1"
                     /translation="MTQQLPRPHATPAVAGGIRLGQATATVNEALQLRRYLLALGLTE
                     HVTRQDGIWRLDPEAAQRWLPAGDTLGLHQRLAARWQPDALELRRQIVVAMLAGPWVF
                     EFPSCAELRAAVRVRERIVRAARQTALAFRTEAAERPAEDWCYHPDTGFTLRPGRGLI
                     AALRKATQPRVSGRLYDFSCYRATEYVILLALAQEAARSNPALLARLQRQWECKAIMS
                     GSFHDTFLREYGHQDTPLPTRYYVPGDRLWFRNPDEASADATGYEGSWVFYLGHGEFS
                     DFWRAGRTYTLESKCLEIYHWRDGLYRDARTGASQIDEGRVAQQVAATLRDPAGHARI
                     QASMQRYREPRGCYTDAGGCIDTTREYPRCICPADCEIDLPDAPI"
     gene            11456..12634
                     /gene="ftsY"
                     /locus_tag="PK242_03225"
     CDS             11456..12634
                     /gene="ftsY"
                     /locus_tag="PK242_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOZ93064.1"
                     /translation="MFSFFRRKKAAEPAPENAVPEAAPADPAAELAEAATPLRADELE
                     AQALAAAPEEGPHPAPSVAIETEAAGAAPTSAEVEFDHESANLAQPTPAARSGWLDRL
                     KSGLKKTGSSITTVFTGTRIDEALYEDLETALLMADAGVPATETLLADLKRRVKEARA
                     TEPAQVRALLQDAIADLLAPLEKPLVVGQYHPTVIMVAGVNGAGKTTSIGKLTRHLAE
                     AGATVLLAAADTFRAAAREQLAIWADRNAVEIISQQGADPSAVAFDAVAAGRARGRDV
                     VLVDTAGRLPTQKHLMEELAKIRRVVTKADVTAPHEVLLVIDGNTGQNALAQVRAFDE
                     AAQLSGLIVTKLDGTAKGGVLCAIAREKPVPVYFIGVGERLEDLQTFRAREFAQALVG
                     "
     gene            12738..13829
                     /locus_tag="PK242_03230"
     CDS             12738..13829
                     /locus_tag="PK242_03230"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ93065.1"
                     /translation="MSVNPPRKHLVLLGAGRAHMQVLGSLARQGSGHLAVTLVAPHPY
                     YIERAMLAGYVAGDYALEQLRLPLDRLIERSGASFVPAHVIALDPDGRRVQLSSGDAL
                     PYDVLSINLEPALERERIEQAMPGARANALFTHPLETFVHLWPQLLALAGERALQVAV
                     VGDGLPAVELALAVAHAVAAPHGSRVTLVAGSAPLLTDPSSALQRRLLARLKQSHITL
                     LHDQCTGIASGVMQLASGATLACDAPLLATGGGTPGWLMQAGLLANDAGEPLVNERLQ
                     SDSHRQIFIVPDDAPAEVGPVLDANLRTAVGGGSFRKAPGEPPRLRIVSCGSGQAVAA
                     WGPLGLEGREVWHWKDRRDRRQLAALFAV"
     gene            complement(14134..14910)
                     /locus_tag="PK242_03235"
     CDS             complement(14134..14910)
                     /locus_tag="PK242_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOZ93066.1"
                     /translation="MHLAPADTIAGLVKGLAVLESFDTERQRLNATQAAQRAGLTRAA
                     ARRHLLTLAHVGYLESDGQFFWLSPRALRLAGAYLASARLPRVVQPALNRLAAATQDV
                     YSAVVLDGDEVVIIARSAGLGAPGTRLLAYGMHLGARLPAHTTSTGRVLLAALPRPAL
                     TDWLAHHQALPRLTPHTTTGTRAFKALLAQVARQDWALSSEENELGVHALAVPLRNAQ
                     GATVAALNVIVPPARFSDALMQQELLPRLREAARELRQML"
     gene            15032..16228
                     /gene="pobA"
                     /locus_tag="PK242_03240"
     CDS             15032..16228
                     /gene="pobA"
                     /locus_tag="PK242_03240"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HOZ93067.1"
                     /translation="MTTTRTQVAIVGAGPSGLLLGQLLHKAGIDAVIVERVSGDYVLG
                     RIRAGILEQVCIDLMDEAGVGARMHQEGLVHGGIELLYDGQRHRVDLNKYSGGKNVMV
                     YGQTELTRDLMDARAAAGLTTVYEAHHTAVHDFDTARPYVTYVKNGVTHRIDCDFIAG
                     CDGFHGVCRASAPRSAIREYEKVYPFGWLGLLSDTPPVCDELIYVNSERGFALCSQRS
                     KTRSRYYLQVPLTHHVEDWSDDAFWQELRLRLDDEARERLVTGPSLEKSIAPLRSFVT
                     EPLRFGRMFLAGDAGHIVPPTGAKGLNLAATDVKYLYDGLVEYYRDRSEAGLDAYSAK
                     ALARIWRAERFSWWFTQLMHRFPHDEQITAKFQHAELDYLMHSEAGLRTIAENYVGLP
                     LDFGQR"
     gene            16393..17304
                     /locus_tag="PK242_03245"
     CDS             16393..17304
                     /locus_tag="PK242_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOZ93068.1"
                     /translation="MPVPAPIRPLRGTRIVSLALNLPGPAALMRLHALGARCLKVEPP
                     GPGRTAAGRPIPGDPMGQYDVGAYGVLHQGIRTVSMDLKSERGRARLATELAGAELLL
                     TSFRPSALAKLGLGWRALHRAYPTLSLVEIVGAPGERAEEPGHDLTYLAEAGLVTGTE
                     LPPTLLADMAGALLAVEAALAALMQARASGRGVHRQVALSDAAHWLALPRNWGLTLPS
                     GAVGGAHAGYRVYPCKDGRVAIAALEPHFASALFKVAGLPAPDLRAPLAPQARDAIAA
                     WALGKTRQQLDALARQHDIPLHTLPRA"
     gene            17310..18011
                     /locus_tag="PK242_03250"
     CDS             17310..18011
                     /locus_tag="PK242_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HOZ93069.1"
                     /translation="MRGEGSIRRWDAARGFGFIQTAERRDVFFHVRDFRGAGAPTEGL
                     RVSFEAIEVGGKGPRAMDVRPLSATAGPAATTPAARPQPTAPRSASATAARARPEQVP
                     SRAHAGPPPAPHGAALAWLLMLGWLGLLAAGAWWQRLPTWLLPVMVVLNLATYLAYAG
                     DKRAAQRGHWRTPEQTLHLLALLGGWPAAWWAQQWLRHKSRKLEFRLAYWVTVVLNCA
                     ALALLVARPTWLPAP"
     gene            18119..19531
                     /locus_tag="PK242_03255"
     CDS             18119..19531
                     /locus_tag="PK242_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOZ93070.1"
                     /translation="MRERGLAPAFGPAVRQQLDTIDGPAEDEGPDIDDLTGLLWCSID
                     NDDSRDLDQLTVSETLPDGGVKLLVAIADVDALVSKDTPIDRHAQINTTSIYTSARIF
                     PMLPEKLSTDLTSLNPHQVRVATVTEMVFAPDGTLLRSHIRRARVRNQAQLAYDAVSA
                     WLEGQGPLPEAADRVPGMDDQLRTQDALAQQLRANRREQGALEFQTLQPRAEFEGERV
                     VAIRQQEQNRARQLIEEFMVATNGVTARFLAGKRRAAIRRVVRSPERWARIAEVAAER
                     GWRLPPDPDAHALEQFLAAERRRDPLRFPDLSLVIVKLMGRGEYVVERPGGPVIGHFG
                     LAVRDYSHSTAPNRRYPDLITGRLLKAALAGERPPYSDTELEFLANRCTSQEDAANKV
                     ERRLRKSEAAMLLEGQIGKVFDGVVTGQSKGSTWLRIFDPPADGKLLASRELKVGDKV
                     RARLVFTHVEHGFIDFEQID"
     gene            19593..19826
                     /locus_tag="PK242_03260"
     CDS             19593..19826
                     /locus_tag="PK242_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93071.1"
                     /translation="MGSVISFYIDQDSPGFRTTTGLLPSPEDDIAPCALSVGDTVSFP
                     NHRNRRYRVVSRHCNAGADMDEPQWLLRLEPLR"
     gene            complement(20020..20622)
                     /locus_tag="PK242_03265"
     CDS             complement(20020..20622)
                     /locus_tag="PK242_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HOZ93072.1"
                     /translation="MTRSASDVLRFWLGAHPLDSAAMQRVQAQWFRKDETFDARLREG
                     FGATIAAARAGRLDRWADTAEGRLALLIVLDQFTRNAFRGQPDGFAGDAQALALALRG
                     IELGHDQAVPPMARIFCYLPLEHAEDATLQARNVALFQALRDAPGAEPRAFFDGTLDY
                     ALRHQDVIARFGRFPHRNAILGRTSTAAELDYLAQPGAGF"
     gene            20742..>22473
                     /gene="fusA"
                     /locus_tag="PK242_03270"
     CDS             20742..>22473
                     /gene="fusA"
                     /locus_tag="PK242_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOZ93073.1"
                     /translation="MPIAAPTVAQIRNLALVGPAASGKTTLAEALLHRAGAIGAMGSV
                     ERGSTVSDHDPQEIKAQHSLQSSLLHLDHDGCRVHLMDTPGLPDFIGQSLPALEAVET
                     CAVVINAATGIEPMARRMMDWAAERQQDRLIIVNKIDAEGVDLPALLADIQAQFGSEC
                     LPLNLPDAGATKVVDCFYNRDGHSDFGAVADAHRALVEQVVEVDGDFVERYLNDGDID
                     AAELHAPLEQALREGHLIPVCFVSARSGAGVAELLDIITRLLPNPIEGNPPDFFNGEG
                     EAATLMHARPDPAAHVLAQVFKISIDPYVGRLAYVRVHQGTITPASQLFIGDGRKPFK
                     VGHLFLQQGKNHVEVPSAGPGDICTIGKIDELHYDAVLHDAAEDAHLHLRPLPFPVPV
                     HGVAIRPKKRGDEQRLWEIMAKLVAEDPCLKIERLASTHETVVYGLGELHLRLLLERL
                     RDGYKFEVDTQPPRIAYRETIAQNAAAQYRHKKQSGGAGQFGEVHLRVEPLARGAGFE
                     FVDQVKGGTIPGQFMPAVEKGVREALAEGVIAGYPVQDLRVIVHDGKHHPVDSKEVAF
                     VTAGKKAVQAA"
BASE COUNT         3362 a         8311 c         7758 g         3042 t
ORIGIN      
        1 ccggcgtggg cgtgatgttc tacgtgctgc tggcgctgcg cgccgagctg cgcgcgcgcg
       61 gccggttcag cgccgccacc cagccgcggc tggacagcct gctgccgctg gtggcgctgg
      121 gcacggtggc cgacgtggtg ccgctggacg ccaacaaccg ccgcctggtg gcgcagggcc
      181 tgcggcgcat ccgcgccggc cagatgccgg tcggcctggc ggccctgttt gcggtcgcgg
      241 gccgggccac ccccagggcc ggcagcttcg acttcggctt tgccctcggc ccgcgcatca
      301 acgccgccgg ccggctggcc gacatgacgc tgggcatcga gctgctgctg accgacgacg
      361 ccgcgcgcgc cgccgagctg gcggccacgc tggacgccat caaccgcgag cggcgcgaga
      421 tcgaaggcgg catgcgcgaa caggcgctgc agatcgccga agggctgttc gagccgcacg
      481 ccgacccccc tgccgccctg gccgtgttcg accccgactt ccacgaaggc gtggtcggca
      541 tcgtcgccgc ccgcctgaag gaccggctgc accgcccgac cttcgtgttc gccgccagcg
      601 gcgcgcccgg acgcgagcac gagctgaagg gctcgggccg ctcgatcgcc ggctttcacc
      661 tgcgcgacgc gctggacctg gtggccaagc gccatcccgg cgtgctgctg cgctttggcg
      721 gccacgccat ggcggccggc tgcaccattg acgaacacca gttcgacacc ttcgagcgcg
      781 ccctgcagca ggtcgccacc gagtggctgg acgccgccac gctgacgcgc cgactgcaca
      841 ccgatggtgc gctgccgccc gaatatcgcc gccccgacgt ggtcgatctg ctgcacgacg
      901 cggtctgggg ccagggtttc gctccgccgg tgttcagcga ggagctggag atcgtcggcc
      961 agcgcctggt gggcgaaaaa cacctggcgc tcaagctgcg tcaccagggg cagccggtcg
     1021 atggcatctg gttcggccgc accgagccgc tgcccgcgcg cgccctgctg gcgttccgac
     1081 tggaggccga cgaatggcag ggcgtgcgcc gcgtacgcct gctgatcgaa gggctgggcg
     1141 cctgatcccg cgagcgaccg tcggagaagg tgcgagcctt ccgacgcgcc tgccatccgg
     1201 gcgcgcgccg cgcgcatgac ggtatgccgg caggtcatga aggctgggcg acaatcaggg
     1261 ttttccaccc ggtgcgccgc gcgcacgttc accgcaagac gtcatggcag ccttcgacga
     1321 cgaaaaagtc ctctcggtcc accactggac cgatcgcctg ttttctttca ccaccacgcg
     1381 cagccagagc ctgcgctttt ccaacggcca cttcaccatg atcggcctgc gggtgaatgg
     1441 caagccgctg ctgcgcgcct actccatcgt cagcccgaac tacgaagaga cgctggagtt
     1501 cctcagcatc aaggtgcccg acggcccgct gaccagccgc ctgcagcaca tccagccggg
     1561 cgacagcatc atcgtcggcc acaagcccac cggcaccctg ctgatcgact acctgctgcc
     1621 cggcaagcgc ctgtacctgt tcggcaccgg caccggcctg gcgccgttca tgagcatcat
     1681 ccgcgacccc gacacctacg agaagttcga tcaggtgatc ctggtgcacg gctgccgcct
     1741 ggtgagcgag ctggcctacc gcgactacat cacccaggag ctgccccggc acgaattcct
     1801 cggcgagctg gtgagcagcc agctcaggta ctaccccacg gtgacgcgcg agcccttccc
     1861 cacccagggc cgcattcccg agctgatcga aagcggccgc atggcgcgcg atctgggtct
     1921 gcccgacctg cacccgcagc tcgaccgcgt gatgctgtgc ggcagccccg agatgctcaa
     1981 gtcgatcaag gaagtgctgg aaaagcgcgg cttcaaggaa ggcaacacca ccacgccggg
     2041 cgacttcgtg gtcgagcggg cgttcgtcga gcagtaaacc ggcctcgcca ccggccgggc
     2101 gcgcgccgcg tcagcgcccg ccggcggcaa aaaacgccac catgcgcgcc gtcgcgtcct
     2161 gagtcacctg cgcgtcgtag acgtagcccc tggccggcaa accgggcttg tcccaggcgt
     2221 gggtggcttg cggatacaga tgccactcga ccggcttgcc cgctgccttc atctgctcca
     2281 gcaagggaaa gcaggcagac gcgggcactt ctgcgtcgcg cccgcccatc agcatgagca
     2341 gcggtcgatc ggtgtccgac agcaccaact ggtattgttt gaacgcgcag ctgccgtagt
     2401 tggcgaccgt ggccgcaaag cgcagctcgg agcccaccag ccgcgcgctc tgcggcgagg
     2461 ccagatgggt cgacacgaaa gccccccagc tgtagcccgc ctgatagatg cgcgacttgt
     2521 cgacgaacgg cttgcccgcc agcagggcaa gtgccgcata ggcatcggcg atgccggcgg
     2581 aatggctgag cacacgcgtg gcgcaactgt cgaagccgcg cggaccgaag ctgtcgatca
     2641 ccagcaccac gtgaccggcc gccagcaggg actgcgcatg ctgcttcatg tgtgcgttgg
     2701 gcgggccctt caggccgccg caggtatgca tcagcacggt ggccgggaac ggcccgtcac
     2761 ccgccggctt gaacaccgtg ttggcgacgt tggtgaacag gccgatcgaa tccttgatct
     2821 catcgctgaa cgcgaccggc ttgagctcca gcggctgcgc catctgagct tgcaggtcct
     2881 gcgcgccgac gccccagtgg gcgagcagca gcgcagtggc caagagcaga cgatcaagag
     2941 gtttgcgctg gtgcatgacg gtctccttgt ggtcaggtgg tggcactccc gacgccatcg
     3001 ggctgccccc acccaggcgc caggcggcgt cttcagggac ggccgcgctc gccgtggcgg
     3061 cgcatcaggt gcgccagcac cggcggcgac acgaactgtg ccacatcgcc gcccagcatg
     3121 ctgatctcgc gcaccagggt gctgctgatg tgctgggtgg cgctgctggg ggtcaggtag
     3181 atggtgtcga cctcgggcac cagggcgcgg ttcatgccgg ccagctgcgc ttcgtaatcg
     3241 aagtcggtca cgctgcgcac gccgcgcacc atcacccgcg cgccctgcgc cacggcaaag
     3301 gcggtcacca gaccggtgaa gggcaccacg ctgacctgcg gcagatcggc cagcgcctcg
     3361 cgcgtgatgg ccagccgctc gtccagcgtg aacagggtct tcttgtggtg cgcggtggcg
     3421 acggcgatga ccacgcgctc gaacagccgc gccgcgcgcc gcacgatgtc ctcgtgtccc
     3481 agggtgatgg ggtcgaaggt gccggggtag acggcgatga cgggctggct catgggtggg
     3541 ctctccggcg cggcgggcgc tgtggcggtg atacggtggc aggcgattat gcggaaggcg
     3601 ccggccgctg cagcaggtgg gcgtgcaccg ccccggcctt gaggtggcgg tgcagtgcca
     3661 ggcccagcgc gtccagcgcg gcatcgtccc agcgttgcgg cgtctccagg tagacgaaac
     3721 cgcccggccg cacggcgcgg gcggcggccg tcagtgccgg cgccagcagc gccggctgat
     3781 cgaacggcgg gtcgagcagc accagatcga cgctggccgg cgccagcgcc gccagcgccg
     3841 ccaccccgtc gcccgcctgc acgcgcacca tggccgcccc caggcgctgt ttcagcgccc
     3901 gcaggccggc cgccaggccg tcgtcgcgct ccaccagcag cacctcggca gcgccgcgcg
     3961 aggccgcctc cagccccagc gcgccggtgc cggcaaaggc atcgaccacc tgccagccgg
     4021 tcagcgtctg gcccagccag ttgaacaggg tttcacgcac gcggtcgggc gtgggacgca
     4081 ggccgggccg atcgggcacc ggcagcaggt ggcggcgcca ctggccgccg atcacgcgca
     4141 cctgccgcgg cagggcggcg cggccacggg ccgcgggttt cgagggctgg gacatgggcg
     4201 cattgtcgcc cgcccgtcgg cgccggcgtg acgtggatca acaaagcgcg ccagcgcggg
     4261 ccgccatgcg gccgccgccg gacactcgga atagactagg gcctgtgaac gctctgcaag
     4321 gggccgtctt gcatggcgtt cgcaggcccc caagcaaatt catccgccac tggaccatcg
     4381 ccatgagcgc caagccccag aacccggtcg ataccgacgc gccgctgacc aacttctcgc
     4441 actgccatgc cggcatcctg cggcatctgg acgacttcgg ccagttgccg gcgctgctgg
     4501 agccggcgca gcggctgcgc cagatcgccg agcgctttgt cgagttcttc cgcgaagcgg
     4561 tgttcgagca ccaccaggaa gaagaacgcg agctgttccc ggccgtgctg gccagcgctg
     4621 cgccgggcgc cgagcgcgaa cgtgtgcagc agatggtgga ccagctcacg cgcgagcacc
     4681 gcgacgtcga ggcgcagtgg aagcggctgg agccgcagat caagaaactg gccaagggcc
     4741 agccggccga cgtcgacccg caggacgtca gaagcctggt ggcgcaatac caggcgcacg
     4801 cccgcttcga ggagcaggag ttcctgccgc tgtccgagca gatcctgtcg cgcaacagcg
     4861 accacatggc ggcgctcgga ctgtcgctgc acatgcggca tcagccggcg ttcctgagtc
     4921 acatctgaat tgctactttt tttgtagctg ctggcgcttg ccctgcaagg ggcaggcgcc
     4981 ttttctttgc acaatccgcg ggccgggcgg gtgccgccgc tcctgcggcc tggcgttcaa
     5041 aaccatgacc catgacccat gacccgttgg tcatcgaagg gcagcgtggt cattggtcat
     5101 tcttagcgcg cgttcgtatc agcgcccggt aaagcgtgcc gggcgttttt cgatgaaggc
     5161 gcgcacgccc tcggcggcgt cctcgctggc ggccaggcgg cgctgcaccg gaccgaagtc
     5221 ggccaccgcg gcggcctggc ccagttcgat cgccttgagc acgttcagtc gcgtggccac
     5281 caccgcctgc ggcgcctggg cggcgatgcg gcgcgccagc gtcagcgcgc tgtccagcag
     5341 ttcggccgcc ggcaccacct tctgcacgaa gtggcaacgc aaagcctcgg cgctgtcgaa
     5401 ctggtcggcc gtcagcaggt gcagcatggc gttgccgacg ccggcacgct cggccatgcg
     5461 cagcgtggcg cccccggccg gcaggatggc gcgctgcact tccatctgcg agaagcggca
     5521 gttgtcggcg gcgatcgcga tgtcggccgc cagcatcagc tcgatgccga cggtgaagca
     5581 gatgccctgc accgccgcga tcatcggttt ctggcgccgc cggtagcccg gcaggcccag
     5641 ctcgtgcggc tccaccaggc cgtagggggt gagcggctcg ccgcgctgca ggtgctccac
     5701 caccaggggc agatccagcc cggcggtgaa gtgttcgcca aaggcgtgca gcacgcccac
     5761 gcgcagcgtc gggtcgtcgt ccagccgggt gtaggcctcg gccagctcgc ggaacatgcg
     5821 cggcgtccag ccattgcgct tggccggacg gttgaggccg atcagcagga tctggtcatc
     5881 gatcacctgg gtgtcgatgc agccttcggg cggcggggtg cgggggatcg gggtggtcat
     5941 gggtgggctc cttcagggca ggctggaatc agatgatcgt cattcgtcac cggtcattcc
     6001 cgttgcgctg gcccgcttcc ggcgccccga ccaccaccgt gaccatgcgc tcgggttgca
     6061 gcttgcgctg cagggcggcg cggatgtcgg ccagggtgac ggcggccacg cgcgcgctcc
     6121 agtgctccag gtagtccagc ggcaaaccgt tccaggcgat gttggcgacg ttggccagca
     6181 gcttgcggtt gccgtccagg cgcagcgcaa agccgccgat caggttgtcc tgagccgcct
     6241 tcagctcgtc ggcgctgggg ccctgggcca cgaagtcggc cagcacctgg cgcaccaccg
     6301 ccaccgccgc gtcggcctgg tcggggcggg tctgcaggcc gatgctgaag gccccggcgt
     6361 gcaggccggg cgcgaagtcg ctgtacacgc tgtaggacag gccgcgctgc tcgcgcactt
     6421 cgcgcatcag gcgcgaggtg aagccgccgc cgcccaggat gtggttgccc accagcaggg
     6481 caaagaagtc ggggtcgctg cgcttgtagc cgggctggcc gatcagcacg tgcgcctgcg
     6541 ccgactggaa cgggatgcgg atctcgcgcg ccgcctgcag cggcgccacc tcggccaccg
     6601 gcggcagcgg cgcacaggcg ccggccgacg gcagtcgccc cagcagctcg cgcaccagcg
     6661 cgtcggcctg cgcgcggtcg accgcgccca ccaggctgac ccgggcgcgg cagggctgca
     6721 catgctggcg ataaaaggcc tgcaggtcgg ccacctggat gcgcgccagc gtctcgggcg
     6781 tgctgtcctg gccgtagggg tggcggccgt acaccgcctg ctggaacgcc agcgccgcct
     6841 gggtggccgg gcgcgtgcgc gcttcctgga tggcggccga caggcgtgcg cgctcgcgct
     6901 gccacacggg ctcgggccag gccggctcgc ccagctcgcg cgcggccagc tgcaccgcct
     6961 ggcgcagcag ctcggcatcg gtcagcgtgc gcagcgcaaa gctcatgcgg tcggcgccgg
     7021 cgctggcgcc gaacgaagcg cccaggtcgg cccaggcctc gccgagctgg ttctcgtcca
     7081 gcgccggctg ctcgccgcgt gcggccacgc ccttgccggc cagcagcgcg gtcgcgctgg
     7141 ccaggccggc ctgcgccggt gggtcgcgcc ggctgccggc gtcgaagtcg agctgcacgt
     7201 ccagcatcgg gatggccggg ctttcgacca gccagacctg ggccccactg ggctgggtcc
     7261 agtgctggat cgggatcgcc gcccacgcgg ataccgaatg aaaaaaggct ccagagcata
     7321 ccaggagcgc gcgaacagct attgttttca tagtcttcat ggcgcttggc gcagaaggtg
     7381 gcaaggggtt gcgaatgccc gaagggagcg cccgacgccg cccttcaatg gcgcgtcggc
     7441 gcgggcgccg ggcgcgtggc gccggcggcg cgcggctgcg gcaccagggt ggcgacggtg
     7501 agctggtcgt cgccgaaata cttgcgcgcc accgcctgca cctgcgccgc cgtgacctgg
     7561 cgcagccgcg ccaccaggcg ctggtcggcg tccagcggca ggccctgcac ccacaggtgg
     7621 cccagctcct gcgcctggcc catcaccgag tcgcgcttgt agacctcgct ggccacccac
     7681 tgggtcttga cgcgcgccag ctcggccggg ctgacgccct gctcggccac gcgcgccacc
     7741 tcggcacgca gcgccgcctc gacctgcgcc gcgctcttgc cggtggccgg cacgccgccg
     7801 agcgtgaacc attgcggccc gcgcccgagc aggccgttgc cggcgctggc cgaatcggcc
     7861 acgcggtccg ggccctgggt cagcgcgcgc tccagccgcg cgccgctgta gccgtccagc
     7921 acggcggcca gcacggtgag ggccagcgcg tcgtcgttgt cggcgcccgg ctccaggccg
     7981 tcgagccgcg gcacgcgaaa ggccagcgac accacggcct gctcggccgg cgccttgacc
     8041 tcgatgcggc gcaggccgag ttgggctggc tcggggcgcg gcttgcgccc gggcagcggg
     8101 cgcgccgcca tgccgccgta gtggcgctgc gccagcgcca gcacggcggc cggctcgacg
     8161 tcgccgacca ccaccacggc ggcgttggcc ggcacgtacc actggcgatg gaaggcgcgt
     8221 gcgtcgtcgg ccgtcatggc ctgcaggtcg cccatccagc ccaccaccgg gcggcggtag
     8281 ggcgaggcga tgaagccgac cgcgcgctgc tgctcgaaca gcagcgcgcg cgggttgtcc
     8341 tcggtgcgca ggcggcgctc ttccttgacc acctccagct ccttggcgaa cacctcgtcg
     8401 ctgtagcgca ggtgggcgaa gcgctcggcc tccagcgcca tcacctcgcc cagcctggcc
     8461 gccggcacct gctggtagta gccggtgtag tccaggctgg tgaaggcgtt ctcgcgcccg
     8521 cccagctgcg ccacgcggcg cgaaaactcg cccggcgcca gccgctcgct gcccttgaac
     8581 atcatgtgct ccagcacatg ggccacgccg ctggtgccgt ccacctcgtc catggcgccc
     8641 acgcgcagcc acagcatgtg caccgccgtc ggggcgcgac ggtcgggttt gacgatcacc
     8701 gtcaggccgt tgtcgagggt gaagcgctgc accgcgccgg cggcggcggc cggcgacgct
     8761 gcatcggtgg cggccggcgg cggcgcggac gcgcaggcgc ccagcagcgc ggccagcagc
     8821 acaggcagca ggaaagcggg tctgaagttc atgtcgtcgt gcagagatcg gttcggcggc
     8881 ccggattccc cgcccgcggc cggcagcggc gattgtcgcc gcgccgccgg ggggccgcgg
     8941 ctgccacaat ggcggcccat gaactcgccc gccgccgccc ccgccacgcc cacccccgaa
     9001 ccgcgcctga cctcgctctc gcatggcggc ggctgcggct gcaagatcgc ccccggcgtg
     9061 ctgtcggcca tcctcaagga caccctggcc atgccgatgc cgcccgagct gctggtaggc
     9121 atccagaccg ccgacgacgc cgccgtctac cgcctcaacg acgagcaggc gctggtggcc
     9181 accaccgact tcttcatgcc catcgtcgac gacccgttcg acttcggccg catcgccgcc
     9241 gccaacgcgc tgtccgacgt ctacgccatg ggcggcacgc ccatcatggc gctggcgctg
     9301 gtgggcatgc cgatcaacgt gctgtcgctg cagaccatcg gccgcatcct ggaaggcggc
     9361 gccagcgtct gtcgcgacgc cggcatcccg gtggccggcg gccacaccat cgactcggtg
     9421 gagccgatct acggcctggt cgccctgggc ctggtgcacc ccgagcgcgt caagaccaac
     9481 agcggcgccc aggtgggcga ccggctggtg ctgggcaagc cgatcggcgt cggcgtgcaa
     9541 tcggccgcgc tgaaaaaggg tgccctgccg gccgccggct acgccgccat gctcgccagc
     9601 accacccagc tcaaccgtgc cggcccggcg ctggcgcggc tgccgggcgt gcacgccatc
     9661 accgacgtca ccggctttgc cctggccggc cacgcgctgg agctggcgcg cggcgccggc
     9721 gtgacggtgc acatcgactg gcccagcgtg cccctgctgc ctggcgtggg ccaactggcc
     9781 gcgcagggca tggtgaccgg cgcctcgggg cgcaactggg ccgcctacgg acacgacgtg
     9841 gcgctgccgg ccggctttgg cgacagccag cgcgccctgc tgaccgaccc ccagaccagc
     9901 ggcggcctgc tggtggcctg cgacgccgcc agcgtggacg aggtgttggc gaccttccgg
     9961 cacagcggct tcgcgcaggc ggcggtgatc ggccgcatcg agcctgccgg cgcctccccg
    10021 ctggtggtgg cctagggcct gttaacgccg aggggcgggc tgtgccgagc ttccctgcgg
    10081 gttgcgccgc aaaggaaccg tctgcggcgt tgcccgcgct tgcaaggcac acgccttggc
    10141 tggcgcacgg gcgccttgca tccagcccct ttgggaagct acgcgatccg aatgcatagc
    10201 gtaagcaggc cctgagccgc gcagcgcatc agggcattcc ccagcagcgg gccggggccg
    10261 gcgtgcgcat actgcgcgcc catgacccag cagctgccgc gcccccacgc cacgccggcc
    10321 gtcgcgggcg gcattcgcct ggggcaggcg acagcgacgg tcaatgaagc gctgcagctg
    10381 cgccgctatc tcctcgcact ggggttgacc gaacatgtga cccgccagga cggcatctgg
    10441 cggcttgacc ccgaagccgc gcaacgctgg ctgcccgcag gcgacaccct ggggctgcac
    10501 cagcggctgg cggcacgctg gcagcccgat gcgctggagc tgcgtcggca gatcgtggtg
    10561 gccatgctgg ccggcccctg ggtgttcgag tttccgtcct gcgccgaact gcgggccgcc
    10621 gtgcgcgtgc gcgaacgcat cgtgcgggcc gctcgccaga ccgccctggc gttccgcacc
    10681 gaggccgccg aaagaccggc cgaagactgg tgctaccacc cggacaccgg tttcacgttg
    10741 cgccccgggc gcggtctgat cgcggccctg cgcaaggcca cccagccccg ggtctcgggc
    10801 cgtctgtacg atttttcctg ctatcgcgcc accgaatacg tcatcctgct ggccctggcc
    10861 caggaagcgg cgcgcagcaa cccggccctg ctggcacgcc tgcagcgtca gtgggaatgc
    10921 aaggccatca tgtcgggcag ctttcacgac accttcctgc gcgaatacgg ccatcaggac
    10981 accccgttgc cgacgcgcta ctacgtcccc ggcgaccggc tgtggtttcg caaccccgac
    11041 gaagcctccg ccgacgccac cgggtacgaa ggctcctggg tgttctacct gggccatggc
    11101 gagttcagcg acttctggcg tgccggcaga acctacacgc tggagagcaa gtgtctggag
    11161 atctaccact ggcgcgacgg cctgtaccgc gacgcccgca ccggcgccag ccagatcgac
    11221 gaaggccggg tggcacagca ggtggccgcc acgctgcgtg accctgccgg gcacgcacgc
    11281 attcaggcgt cgatgcagcg ctaccgcgaa cccaggggct gctacaccga cgccggcggc
    11341 tgcatcgaca ccacgcgcga atacccgcgc tgcatctgcc ccgccgactg cgagatcgac
    11401 ctgcccgacg ccccgatctg acctgcacca cgcatgggct gcctagaatc ggtccatgtt
    11461 cagcttcttc cgccgcaaga aagccgccga gccggcaccc gaaaacgccg tacccgaggc
    11521 cgcacccgcc gacccggcgg ccgaactggc cgaagcggcc acgcccctga gggcggacga
    11581 actggaagcc caggcgctgg ccgccgcgcc cgaggagggg ccacatccag cgccctcggt
    11641 tgctatcgaa acagaagctg ctggcgcagc acccacaagc gctgaggtcg aatttgatca
    11701 tgaatccgcg aacctggcgc agcccacccc cgccgcccgc agcggctggc tcgaccgcct
    11761 gaagagcggc ctgaagaaaa ccggcagcag catcaccacc gtcttcaccg gcacgcgcat
    11821 cgacgaggcg ttgtacgaag acttggaaac cgcgctgctg atggccgacg ccggcgtgcc
    11881 ggccaccgaa accctgcttg ccgacctgaa gcgccgcgtc aaggaggcgc gcgccaccga
    11941 gccggcccag gtcagggcgc tgctgcagga cgccatcgcc gacctgctgg cgccgctgga
    12001 aaagccgctg gtggtggggc agtaccaccc caccgtcatc atggtggccg gcgtcaacgg
    12061 tgccggcaag accaccagca tcggcaagct cacgcgccac ctggccgagg ccggcgccac
    12121 ggtgctgctg gcggccgccg acacctttcg cgcggcggcg cgcgagcagc tggcgatctg
    12181 ggccgaccgc aacgcggtcg aaatcatcag ccagcagggc gccgacccct cggccgtggc
    12241 cttcgacgcc gtggccgccg gccgcgcgcg cggccgcgac gtggtgctgg tcgacaccgc
    12301 cggccgcctg ccgacgcaaa agcacctgat ggaagagctg gccaagatcc gccgcgtggt
    12361 caccaaggcc gacgtcaccg cgccgcacga agtgctgctg gtgatcgacg gcaacaccgg
    12421 gcagaacgcg ctggcgcagg tgcgcgcctt cgacgaggcg gcccagctca gcgggctgat
    12481 cgtcaccaag ctcgacggca ccgccaaggg cggcgtgctg tgcgccatcg cgcgcgaaaa
    12541 gccggtgccg gtgtacttca tcggcgtggg cgagcggctg gaagacctgc agaccttccg
    12601 cgcacgcgag tttgcccagg cgctggtcgg ctgagccgct ctgaacgcgc cccccaggcc
    12661 caccttctgt gcgccgcccg ctgcaaccga atgcaaacac cggcgtgccc ccgcgccctg
    12721 tcgatacact cgggcgcatg agcgtcaatc ccccccggaa acacctggtg ctgctgggcg
    12781 cgggccgtgc ccacatgcag gtgctgggct cgctggcgcg ccagggcagt ggacatctgg
    12841 cggtcaccct ggtggccccg cacccctact acatcgaacg cgccatgctg gccggctacg
    12901 tggccggcga ctacgcgctg gaacagcttc gcctgccgct cgaccggctg atcgaacgca
    12961 gcggcgccag cttcgtgccg gcgcatgtga tcgcgctcga ccccgacggg cgccgcgtgc
    13021 agctgtccag cggcgacgcc ctgccctacg acgtgctgtc gatcaacctg gagccggcgc
    13081 tggagcgcga gcgcatcgaa caggccatgc ccggcgcccg cgccaacgcg ctgttcaccc
    13141 atccgctgga gaccttcgtg cacctgtggc cgcagctgct ggcgctggcc ggcgagcgcg
    13201 cgctgcaggt ggcggtggtg ggcgacggcc tgccggcggt cgagctggcc ctggccgtgg
    13261 cacacgcagt ggcggcgccg cacggcagcc gcgtcaccct ggtggccggc agcgcgccgc
    13321 tgctgaccga cccgtccagc gcgctgcagc ggcgcctgct ggcgcgcctg aagcagtcgc
    13381 acatcacgct gctgcacgac cagtgcaccg gcatcgccag cggcgtcatg caactggcca
    13441 gcggcgccac cctggcctgc gacgcgccgc tgctggccac cggcggcggc acgcccggct
    13501 ggctgatgca ggccgggctg ctggccaacg acgccggcga accgctggtc aacgaacggc
    13561 tgcagagcga cagccaccgc cagatcttca tcgtgcccga cgacgcaccg gccgaagtcg
    13621 gcccggtgct cgacgccaac ctgcgcaccg ccgtgggcgg cggcagcttc cgcaaggccc
    13681 ccggcgagcc gccgcgcctg cgcatcgtca gctgcggcag cggccaggcg gtggccgcct
    13741 ggggcccgct ggggctggaa ggccgcgaag tctggcactg gaaggaccgg cgcgaccggc
    13801 gccagctggc ggcgctgttt gccgtctgat ccgaccgctg catcacgaat gacctcgctg
    13861 cgcttgcatg gcgccctcac ccagcaggtg aaggctccga agtggcgcaa ggtcagagtt
    13921 catgcggcat tcccaagggt tgcaagcggt caaccgccgt ttctaggagg ctgtcgggct
    13981 tggagcgccg aaagcgagaa tcggccccag cgagaccagt ttttgccgga tttgcagccc
    14041 aataggtgag ctattgggca aaaagccggt gaaaaatggg ccgttgcggt cgatttgcag
    14101 ccggcgatct ccaagtccga cagcctccta ggttcatagc atctggcgca gctcgcgcgc
    14161 ggcttcgcgc aggcgtggca gcagctcctg ctgcatcagc gcgtcgctga agcgcgccgg
    14221 cggcacgatg acgttgagcg ccgccacggt ggcgccctgg gcgttgcgca gcggcaccgc
    14281 cagcgcgtgc acgccgagct cgttttcctc gctcgacagc gcccagtcct ggcgcgccac
    14341 ctgcgccagc agcgccttga aggcgcgcgt gccggtggtg gtgtgcgggg tcaggcgcgg
    14401 cagcgcctgg tggtgcgcca gccagtcggt cagcgcgggc cggggcagcg ccgccagcag
    14461 cacgcgcccg gtggacgtgg tgtgcgccgg caggcgcgcg cccaggtgca tgccgtaggc
    14521 cagcagccgc gtgccgggcg cccccaggcc ggcgctgcgg gcgatgatca ccacctcgtc
    14581 gccgtccagc accacggccg aatacacgtc ctgcgtggcc gccgccaggc ggttgagcgc
    14641 cggctggacg acgcgcggca gccgcgccga cgccaggtag gcgccggcca gccgcagcgc
    14701 gcgcgggctg agccagaaga actggccgtc gctttccagg tagccgacat gggccagcgt
    14761 cagcagatgg cggcgcgcgg cggcgcgcgt caggccggcg cgctgcgcgg cctgggtggc
    14821 gttcaggcgc tggcgttcgg tgtcgaaact ctccagcacc gccagcccct tgacgaggcc
    14881 ggcaatggtg tccgcaggcg ccaggtgcat tgcgcgatga tcgcacagtt tgcgcggtga
    14941 tcgcgcagcg gtgcggtgcc cgcactggcg gccggcgccg ccggcgccta agctcacggc
    15001 tttcccgcct tcacgcacga ggagacacac cgtgaccacc acccgcaccc aggtagccat
    15061 cgtcggcgcc ggcccgtccg gcctgctgct cggccagttg ctgcacaagg ccggcatcga
    15121 cgccgtgatc gtcgagcgcg tcagcggcga ctacgtgctg ggccgcatcc gcgccggcat
    15181 cctggagcag gtctgcatcg acctgatgga cgaagccggc gtgggcgcgc gcatgcacca
    15241 ggaaggcctg gtgcatggcg gcatcgaatt gctgtacgac ggccagcgcc atcgggtcga
    15301 cctgaacaag tacagcggcg gcaagaacgt gatggtgtac ggccagaccg agctgacgcg
    15361 cgacctgatg gacgcgcgcg ccgccgccgg cctgaccacc gtctacgaag cgcaccacac
    15421 cgcggtgcac gacttcgaca cggctcggcc gtacgtcacc tacgtgaaga acggcgtcac
    15481 acaccgcatc gactgcgact tcatcgccgg ctgcgacggt tttcacggtg tgtgccgcgc
    15541 cagcgcgccg cgcagcgcca tccgcgaata cgagaaggtc tacccgttcg gctggctggg
    15601 cctgctgtcg gacacgccgc cggtctgcga cgagctcatc tacgtcaact ccgagcgcgg
    15661 ttttgccctg tgcagccagc gcagcaagac gcgcagccgc tactacctgc aggtgccgct
    15721 gacgcaccac gtggaggact ggagcgacga cgccttctgg caggaactgc ggctgcgcct
    15781 ggacgacgag gcgcgcgagc gcctggtcac cggccccagc ctggaaaaga gcatcgcccc
    15841 gctgcgcagc ttcgtcaccg agccgctgcg ctttggtcgc atgttcctgg ccggcgacgc
    15901 cggccacatc gtgccgccca ccggcgccaa gggcctgaac ctggcggcca ccgacgtcaa
    15961 gtacctgtac gacggcctgg tcgagtacta ccgcgatcgc agcgaggccg gcctggacgc
    16021 ctactcggcc aaggccctgg cccgcatctg gcgcgccgag cgcttctcct ggtggttcac
    16081 ccagctgatg caccgcttcc cgcacgacga gcagatcacc gccaagttcc agcacgccga
    16141 gctggactac ctgatgcatt ccgaagccgg cctgcgcacc atcgccgaaa actacgtcgg
    16201 cctgccgctg gacttcggcc agcgctgacg cgccccacgg ccggccaagg ccagcgcggc
    16261 caccagacgc gagcgtgggt cggcgctcgc gcgcatggca ggcggccggc cgccgccttc
    16321 gggttcaagg caaaatggct tgcggcgcgc caccgtggtg cgccagcagc tatcactttg
    16381 caagtccatc ccatgcctgt acctgccccc atccgacccc tgcgcggcac gcgcatcgtc
    16441 agcctggcgc tcaacctgcc cggccccgcg gcgctgatgc gcctgcacgc gctgggcgcg
    16501 cgctgcctca aggtcgagcc gcccggcccc ggccgcacgg cggccgggcg gccgattccg
    16561 ggcgacccga tgggccagta cgacgtcggc gcctacggcg tgctgcacca gggcatccgc
    16621 accgtgtcga tggacctcaa aagcgagcgc gggcgcgccc ggctggccac cgagctggcg
    16681 ggcgccgagc tgctgctgac ctcgtttcgc ccctcggcgc tggccaagct gggcctgggc
    16741 tggcgcgcgc tgcaccgcgc gtacccgacg ctgtcgctgg tggaaatcgt cggcgcgccg
    16801 ggcgagcgcg ccgaagagcc cggccacgac ctgacctacc tggccgaagc cggcctggtc
    16861 accggcaccg agctgccccc caccctgctg gccgacatgg ccggcgcgct gctggcggtg
    16921 gaggccgcgc tcgccgccct gatgcaggcg cgcgccagcg gccgcggcgt gcaccgccag
    16981 gtggccctgt cggacgccgc gcactggctg gcgctgccgc gcaactgggg cctgaccctg
    17041 cccagcggcg ccgtcggcgg cgcgcacgcg ggctaccgcg tctacccctg caaggacggg
    17101 cgcgtggcca tcgccgcgct ggagccgcac tttgcctccg cgctgttcaa ggtggcgggc
    17161 ctgcccgcgc ccgacctgcg cgcccccttg gcgccgcagg cgcgcgacgc catcgccgcc
    17221 tgggcgctgg gcaagacgcg ccagcagctc gacgcgctgg cgcgccagca cgacatcccg
    17281 ctgcacaccc tgccgcgcgc ctgagcccca tgcgcggcga aggcagcatc cggcgctggg
    17341 atgcggcgcg cggcttcggc ttcatccaga ccgccgagcg gcgcgacgtg ttcttccacg
    17401 tgcgcgactt ccgtggcgcc ggcgcgccga ccgaaggcct gcgcgtcagc ttcgaagcga
    17461 tcgaggtcgg cggcaaaggc ccgcgcgcca tggacgtgcg gccactgtcg gccaccgccg
    17521 gcccggccgc gacgaccccg gcggcgcgcc cgcagcccac ggcgccccgc agcgccagcg
    17581 ccacggccgc gcgtgcccgg ccggagcagg tgcccagccg ggcgcacgcc ggcccgccgc
    17641 ccgccccgca cggcgccgcg ctggcctggc tgctgatgct gggctggctg ggcctgctgg
    17701 cggccggcgc ctggtggcaa cgcctgccga cctggctgtt gccggtcatg gtggtgctca
    17761 acctggccac ctacctggcc tacgccggcg acaagcgcgc cgcgcaacgc ggccactggc
    17821 gcacccccga gcagacgctg cacctgctgg ccctgctggg cggctggccc gccgcctggt
    17881 gggcgcagca gtggctgcgc cacaaatcgc gcaagctcga attccgcctc gcctactggg
    17941 tcaccgtggt gctgaactgc gccgccctgg cgctgctggt ggcgcgcccc acgtggctgc
    18001 ccgcaccctg acgccccacc tatgatgtca cccggacgcg cgccaccagg gagccgacgt
    18061 gaacaacaac ctccatcccc accagaagga agagctggcc gagctggccg tgatcgccat
    18121 gcgcgagcgc ggactggcgc ccgcgttcgg ccccgccgtg cgccagcagc tcgacaccat
    18181 cgacggcccg gccgaagacg aaggccccga catcgacgac ctgaccgggc tgctgtggtg
    18241 ctcgatcgac aacgacgatt cgcgcgacct cgaccagctc accgtcagcg aaaccctgcc
    18301 cgacggcggc gtgaagctgc tggtggccat cgccgacgtc gacgccctgg tgagcaagga
    18361 cacgccgatc gaccgccacg cgcagatcaa caccaccagc atctacacct cggcgcgcat
    18421 ctttccgatg ctgccggaaa agctcagcac cgacctgacc tcgctcaacc cgcaccaggt
    18481 gcgcgtcgcc accgtcaccg aaatggtgtt cgcccccgac ggcacgctgc tgcgcagcca
    18541 catccggcgc gcgcgcgtgc gcaaccaggc gcagctggcc tacgacgccg tgtcggcctg
    18601 gctcgaaggc cagggcccgc tgcccgaagc ggccgaccgc gtgcccggca tggacgacca
    18661 gctgcgcacc caggacgcgc tggcgcagca gctgcgcgcc aaccggcgcg agcagggtgc
    18721 gctggagttc cagaccctgc agccacgcgc cgaattcgaa ggcgagcgcg tggtcgccat
    18781 ccgccagcag gagcagaacc gcgcgcgcca gttgatcgag gagttcatgg tcgccaccaa
    18841 cggtgtgacg gcgcgctttc tggccggcaa gcggcgcgcc gccatccgcc gcgtggtgcg
    18901 ctcgcccgag cgctgggcgc gcatcgccga agtcgcggcc gagcgcggct ggcgcctgcc
    18961 accggacccc gacgcgcacg cactggagca gttcctggcc gccgagcgcc ggcgcgaccc
    19021 gctgcgcttt cccgacctgt cgctcgtcat cgtcaagctg atggggcgcg gcgaatacgt
    19081 ggtcgagcgc ccgggcgggc cggtgatcgg ccacttcggg ctggcggtgc gcgactactc
    19141 gcactccacc gcgcccaacc ggcgctaccc cgacctgatc accggccggc tgctgaaggc
    19201 cgcgctggcg ggcgagcggc cgccctactc cgacaccgag ctcgaattcc tggccaaccg
    19261 ctgcaccagc caggaagacg cggccaacaa ggtcgagcgg cgcctgcgca agtccgaagc
    19321 cgcgatgctg ctggaaggcc agatcggcaa ggtgttcgac ggcgtggtga ccggccagtc
    19381 caagggcagc acctggctgc gcatcttcga cccgccggcc gacggcaagc tgctggccag
    19441 ccgcgaactg aaggtcggcg acaaggtgcg cgcgcgcctg gtgttcaccc acgtcgagca
    19501 cggcttcatc gacttcgagc agatcgactg accccctggc caacgccggc cgcgcgctgc
    19561 acaatggccg cacctgtggc gaggaggcca gcatgggcag cgtgatcagc ttctacatcg
    19621 accaggacag ccccggtttt cgcaccacca ccggcctgct gcccagcccc gaggacgaca
    19681 tcgcaccctg cgcgctgagc gtgggcgaca ccgtcagctt tccgaaccac cgcaatcgcc
    19741 gctaccgcgt ggtcagccgc cattgcaacg ccggcgccga catggacgag ccgcagtggc
    19801 tgctgcggct cgaaccgctg cgctgacaga tcctgttttt atagctgccg gcgcttgcca
    19861 gacaagcgcc ggcgccattt tttgcttgca aatggcgatg ggccctgggg ccgcctccac
    19921 cgcttcgcgt tcaagcccat gacaccggcc gcaccggccg gacccacgac cccttggtca
    19981 tcgaagcgca gcgaggtcat gcgccatgca gcgctcgcat cagaaccccg cccccggctg
    20041 cgccagatag tccagctcgg ccgccgtgct ggtgcgcccg aggatggcgt tgcggtgcgg
    20101 aaagcggcca aagcgcgcga tcacgtcctg gtgccggagc gcgtagtcca gcgtgccgtc
    20161 gaagaacgcc cgcggctcgg cgccgggcgc gtcgcgcagc gcctgaaaca gcgccacgtt
    20221 gcgcgcctgc agggtggcgt cctcggcgtg ctccagcggc aggtagcaga agatgcgcgc
    20281 catcggcggc accgcctggt cgtgccccag ctcgatgccg cgcagcgcca gggccagcgc
    20341 ctgcgcgtcg ccggcaaaac cgtcgggctg gccgcgaaag gcgttgcggg tgaactggtc
    20401 aagcacgatc agcagcgcca ggcgcccttc ggcagtgtcg gcccagcggt cgagccgccc
    20461 ggcgcgcgcc gcggcgatgg tggcgccaaa gccttcgcgc aaacgggcgt cgaaggtctc
    20521 gtccttgcga aaccactggg cctgcacgcg ctgcatggcg gcgctgtcca gtgggtgggc
    20581 gccgagccag aagcgcagca cgtcggaggc ggatcgggtc atgccggcat tgtggcgccg
    20641 acccgaccgg cccgctgacc ggcatcagga aatgcccgca cgccccttgt gccgggccgg
    20701 ccgccacact acgctgcgag aaaccgagga gacccagagc catgcccatt gccgccccca
    20761 ccgtcgcaca gatccgcaac ctggccctgg tcgggcccgc cgccagcggc aagaccacgc
    20821 tggccgaagc cctgctgcac cgggccggtg ccatcggcgc catgggcagc gtagagcgcg
    20881 gcagcaccgt gagcgaccac gacccgcagg agatcaaggc ccagcattcg ctgcaatcgt
    20941 cgctgctgca cctggaccac gacggttgcc gcgtgcacct gatggacacc cccggcctgc
    21001 ccgatttcat aggccagagc ctgccggcgc tggaggcggt ggaaacctgc gccgtcgtca
    21061 tcaacgccgc caccggcatc gagcccatgg cccggcgcat gatggactgg gccgccgagc
    21121 gccagcagga ccgcctgatc atcgtcaaca agatcgacgc cgaaggggtg gacctgcccg
    21181 ccctgctggc cgacatccag gcccagttcg gcagcgaatg cctgccgctc aatctgcccg
    21241 acgccggcgc gaccaaggtg gtggattgct tctacaaccg tgacggccac agcgacttcg
    21301 gcgccgtggc cgacgcccac cgcgcgctgg tcgagcaggt ggtcgaggtc gatggcgact
    21361 tcgtcgagcg ctatctgaac gacggcgaca tcgacgccgc cgagctgcac gccccgctgg
    21421 agcaggcgct gcgcgagggc cacctgatcc cggtgtgctt cgtctcggcg cgcagcggcg
    21481 ccggcgtggc cgagctgctc gacatcatca cccgcctgct gcccaacccg atcgagggca
    21541 atccgcccga cttcttcaac ggcgaaggcg aggccgccac cctgatgcac gcccgccccg
    21601 acccggcggc ccatgtgctg gcgcaggtgt tcaagatcag catcgacccc tacgtggggc
    21661 gcctggccta tgtgcgcgtg caccagggca ccatcacccc ggcgtcgcag ctgttcatcg
    21721 gcgacggacg caagccgttc aaggtcggcc atctgttcct gcagcagggc aagaaccatg
    21781 tggaggtgcc cagcgccggg ccgggcgaca tctgcaccat aggcaagatc gacgagctgc
    21841 actacgacgc cgtgctgcac gacgccgccg aagacgccca cctgcacctg cgcccgctgc
    21901 catttccggt gccggtgcac ggcgtggcca tccggcccaa gaagcgcggc gacgaacagc
    21961 gcctgtggga aatcatggcc aaactcgtgg ccgaagaccc ctgcctgaag atcgagcgcc
    22021 tggccagcac gcacgaaacc gtggtctacg gcttgggcga gctgcacctg cgcctgctgc
    22081 tcgaacgcct gcgggacggc tacaaattcg aggtcgacac ccagccgccg cgcatcgcct
    22141 accgcgaaac catcgcccag aacgccgccg cgcagtaccg ccacaaaaag cagagcggcg
    22201 gcgccggcca gtttggcgag gtgcacctgc gcgtcgagcc gctggcgcgc ggcgccggct
    22261 tcgagtttgt cgaccaggtg aagggcggca ccatccccgg ccagttcatg cccgcggtgg
    22321 aaaaaggcgt gcgcgaggcc ttggccgagg gcgtcatcgc cggctacccg gtgcaggacc
    22381 tgcgcgtgat cgtgcacgac ggcaagcacc acccggtgga cagcaaggag gtggccttcg
    22441 tcaccgccgg caagaaggcc gtgcaggccg cca
//
LOCUS       DAOOOG010000022        21378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_2402_length_21378_cov_27.981241, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000022 DAOOOG010000000
VERSION     DAOOOG010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 21378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21378
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2402_length_21378_cov_27.981241"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..4196)
                     /gene="purL"
                     /locus_tag="PK242_03275"
                     /gene_synonym="purI"
     CDS             complement(138..4196)
                     /gene="purL"
                     /locus_tag="PK242_03275"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOZ93074.1"
                     /translation="MTLHIHFFDGGNALSDFRLRQLLPRLQAVAPAVEGVAARHVHLA
                     GFDAAPAAAELERLAALLDGGDAPAGEWPQGEGLVVTPRLGTVSPWASKATDIARNCG
                     LALHRLERLVEYRIWLKPALIKRQQLSQQERTAIAGLLHDRMTESVLPDREAARALLS
                     TLAPEPLAQVDVLGGGRAALERANRDWGLALAADEIDYLLDAFTRLGRNPTDVELMMF
                     AQANSEHCRHKIFNAQFSIDGVAQERSLFAMIRNTHQASPQHTVVAYSDNAAVMEGGE
                     VQAFQVKSPFEADQASAGSYQKQSMRRHVLMKVETHNHPTAISPFPGASTGAGGEIRD
                     EGATGRGAQPKAGLTGFSVSKLWDSPHGRPEHMASALQIMTEGPLGGAAFNNEFGRPN
                     LLGYFREYELNVGAPGHQVLRGYHKPIMIAGGLGHIDARLTHKLEFPAGSLLIQLGGP
                     GMRIGMGGGAASSLASGANAAQLDFDSVQRGNPEIQRRAQEVITQCQALGAANPILAI
                     HDVGAGGLSNAFPELVNDAGRGARFDLRAVPLEESGLAPKEIWCNESQERYVLAIAPE
                     SLPLFEQLCARERCPLAVVGVATDERELIVADDDRDPAPVRMPMEVLLGKPPKMQRDV
                     QRVQRQFTPLQLDELKLQDAVIEVLSHPTVASKRFLVTIGDRTVGGLTHRDQMVGPWQ
                     VPVADCAVTLADYAGFAGEAMSMGERTPLAALDAPASARMAVAEAITNLLAAPIELPR
                     VKLSANWMAACGEPGEDADLYAAVQAVGMALCPQLGIAIPVGKDSLSMRTQWQDADGH
                     ARKVVSPVSLIASAFVSLADVRGTLTPQLDRHEPDSSLILIDLGHGRHRLAGSVLAQT
                     LGQSGCPQVDGVPDLDDPQDLKALVAAINQLRAEGRILAYHDRSDGGLLACVAEMAFA
                     GQVGVALNIDLLVNEGDGISDSRMESGEGKDWARQVSGRRDLLTLKALFAEELGVVLQ
                     VATRERDAVLQVLRAHGLARLSHVIGKTRPEASPIAKGKGCLSVWRDTREVFSASLHD
                     LQQVWDSVSWRIARERDHPDCADAEHAAAGAPGDPGLHLALSEEFKQKLALAQSEQAP
                     AATKNIVIGGARPKVAVLREQGVNSHVEMAWAFSEAGFQAHDVHMTDLQSGRVRLADF
                     RGLVVCGGFSYGDTLGAGVGWARSITFHAGLSAQFQAFFDRRDTFGLGVCNGCQMFAE
                     LADIIPGAQHWPRFTTNRSERFEARLSMVEVLDSPSILLAGMAGSRLPIAVSHGEGYA
                     NFSRRGDAGQVIAAMRFVDHHGQPTERYPFNPNGSLGGLTAVTTADGRFTAMMPHPER
                     VFRNIQMSWTDGDRSDFSPWMQIWRNARRWVG"
     gene            4364..5185
                     /gene="map"
                     /locus_tag="PK242_03280"
     CDS             4364..5185
                     /gene="map"
                     /locus_tag="PK242_03280"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOZ93075.1"
                     /translation="MSITIKDEAGIAAMREACRLAAEVLDTLTPLIQPGITTLEIDRL
                     AAQAMAAQGTRSATVGYQPPGYPPYPAHVCTSVNQVVCHGIPADKPLKKGDIVNVDVT
                     VITPDGWYGDTSRMFEIGDCSIAARRLCALTYQAMWLGIEQVRAGARLGDIGHAIQKF
                     VEGHGLSVVREFCGHGIGQNFHEEPQVLHYGRPGTGVELEPGMIFTIEPMVNVGRRDI
                     KELPDGWTIVTKDRSLSAQWEHTVLVTPTGYEVLSLSAGAPAIPDFVKGVARAAA"
     gene            5228..7882
                     /locus_tag="PK242_03285"
     CDS             5228..7882
                     /locus_tag="PK242_03285"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HOZ93076.1"
                     /translation="MSERAAASCAEAPPGDYRGRKKALLDALRASGPGTRGVRAALTR
                     LSALADDALRALWAQRGFDDGPALLAVGGYGRGELFPYSDIDVLLLLPDGCDAAQDAA
                     LRARIEGFITDCWDAGLEIGSAVRSIGDCLQAGAEDVTVQTALLEARCMAGSRALFDA
                     FRQRHQAALAPLDFFSAKRLELRQRHVKYEDTPYALEPNCKESPGGLRDLQIILWTAR
                     AAGIGGRWDDLHRAGLVTAHELRQLRHNEALLSSIRCRLHLVAGRREDRLVFDLQNAV
                     AESFGLSHRLAADGRVLLRASEVLMRRYYWAAKAVTQLNQILLQNLEEHLRAAHGEPA
                     PALRRINPRFFDRDGLIEVASDELYQRQPHAVLETFLLYTQTPGVKGLSARTLRALYN
                     ARSVMNHAFRADPVNRRTFLAILQQPQGITHAMRLMNQTSVLGRTLWVFRRIVGQMQH
                     DLFHVYTVDQHILMVLRNVRRFFMAEHAHEYPFCSQLAAGWDKPWLLYVAALFHDIAK
                     GRGGDHSRLGALEVRRFCAEHGVAGADAELLEFLVREHLSMSRIAQSEDLSDPEVIAA
                     FARQVGNERQLTALYLLTVADLRGTSPKVWNAWKGKLLEDLYRLTVRSLGGRAPDADA
                     VIEARKREALRELTPAGGPPALPPPLWEQLQVSYFMRHDAGDIAWHARELSPALAGSA
                     ATAPDQPLRATTVRARLSPVGEGLQVLVHASDRPDLFARICGWFERSGFSILEAKIHT
                     TRDVWALDTFQIVRPEAPEDYAEFIDAVQAELPPAIDSQGPLPAPRLGRLSRRVRSFP
                     VAPRVDLRPDEKGQRWLLSVTASDRMGLLYGIARVLAAHQIDVELARITTLGERVEDS
                     FLLQGAQLQHARTQLAIEQELLDVLEPA"
     gene            8247..9083
                     /locus_tag="PK242_03290"
     CDS             8247..9083
                     /locus_tag="PK242_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOZ93077.1"
                     /translation="MNPLASPSRRLLLASAAATALLWGCSKTETPAPAAPEPAAPAAA
                     TAPGLPARIVIGLDDNFPPMGFRDDKNQLVGFDIDLAREAGKRLGIEVEFKPIDWNAK
                     EAELSGKRVDVLWNGLTITEERKKNIAFTAPYMQNHQIVVVKADAAIKAKADLAGKVV
                     GVQDGSSAVEAIEKDAATAQSFKQLKKYGDNVTALMDLSAGRVDAVVLDEVVGRYYSA
                     KKPGEYAVLDEHFGTEDYGVGLRQDDAALRTRLDEVLTAMKGDGSGARIAEQWFGKNI
                     LK"
     gene            9257..9910
                     /locus_tag="PK242_03295"
     CDS             9257..9910
                     /locus_tag="PK242_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOZ93078.1"
                     /translation="MDYLLRILPPLLEGAGVTLGLFVVTLALSLPLGLALALARLSRW
                     GALRRAVSGFVWLMRGTPLMLQLLFVYYALPFVPVIGVRLPDYPAAVVAFTLNYAAYF
                     AEVLRAGIQSIDRGQYEGAKALGMSYAQTMRRIVMPQVWRGSLPPLANETITLVKDTS
                     LIYVLALNDLLRAARGIVQRDFSITPFVVAAAFYLLMTLVLTWIFQRLERRHAAHVA"
     gene            9900..10676
                     /locus_tag="PK242_03300"
     CDS             9900..10676
                     /locus_tag="PK242_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOZ93079.1"
                     /translation="MSPEQRPPMVRASGLHKHFGALHVLRGVSLTVERGEVVAILGPS
                     GSGKSTLLRCLNHLETIERGGIEIAGETLASADAAGASSYPSDSEVRRICQRTGMVFQ
                     HFNLFPHLSVLGNLIEAPITVRKLARAEVVPRAEALLAKVGLADKRDVYPGRLSGGQK
                     QRVAIARALAMQPDILLFDEPTSALDPELTGEVLRTMRQLAEERMTMLVVTHEMAFAR
                     EVAHRVVFMDAGQIVEDRPAADFFAAPRHERARAFLAHML"
     gene            complement(10704..15185)
                     /locus_tag="PK242_03305"
     CDS             complement(10704..15185)
                     /locus_tag="PK242_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HOZ93080.1"
                     /translation="MSNAPPRLTIDFPDSLPVSARRAEIEAALRAHQVVIVCGETGSG
                     KTTQLPKIALAMGRGRLNARPGERGKLIGHTQPRRIAATSVAKRIAEELRTPLGEVVG
                     YKVRFGDRLTPGASVKLMTDGILLAETQTDPLLNAYDTLIIDEAHERSLNIDFLLGYI
                     KQILPRRPDLKLIVTSATIDADRFARHFAMPRPAPGRPKPGVAPSGGSDPHEVGERGG
                     LVPAPGRPKPGVAPSGGSDPHEVGERGGLVPAPGRPKPGVAPSGGSDPHEVGERGGLV
                     PAPVLQVSGRMYPVEQRWRPFEESREHDLNDAIAAAVDELWVGPPQGDILVFLPGERE
                     IREAADHLRAHLSHRPLTRGAEVLPLFARLSQAEQDRVFEPHGAPRIVLATNVAETSL
                     TVPGIRYVIDPGTARVKRYSYRSKVEQLLVEPISQAAAGQRAGRCGRVADGICIRLYD
                     EQDFAGRPRFTDPEILRSSLAAVILRMKSLRLGGEGGRVEDFPFLDMPSGRAIADGVQ
                     LLQELGALDEAQQLTALGTELARLPLDPRVGRMILEARARGALREVLVIAAALSVPDV
                     RDRPLEQQAQADQAHAKFDDEKSEFSGYLRLWKWLHDARGGTGPHASRFAAALPPRGA
                     AAGLGRPGAGRSLPMTRAQMAASEGQTAARKPANAAALPVAQRAAAALAQARQTLSKK
                     ELPALSGQGLDSDLIQNAEASAVHKLSNRQWEQLLRQNFISVRRVREWRDIHTQLHTV
                     VAEHKWRENDQPSGYEALHKSLLAGLLGNVGCKLEGDDAQSGEYLGARGIKFHRHPGA
                     HLSKRPGKWIVCAELVETTRLFGRGIAAIEPQWLEEVGGHLLKKQLLEPHWEKKAQDV
                     VALERATLYGLLVYGGRRRSFGAVDPRAAREIFIRAALVGGDWPEEWARRLPFLLANQ
                     RTIAKVEELEHKSRRQDVLVDEELIYAFYDSQVPADICNGRDFERWWREASRAKPNLL
                     LLTREELMRHEAAGITTSAFPKMVRLGGVDCAASYLHQPGDARDGLTVTVPLFVLNQV
                     SEARCEWLVPGMLKDKIQALLKSLPQKPRSRFVPLPDSAARLAEILGAPEVFGHGSLT
                     DALLRRVREATSLDVKRSDFKLDMLPAHLFMNFRVVDEHGRQRGMGRHLAALKAELGG
                     EARGAFQALAALKGAVPGAAPAAAPGPQDSEQNQAPAQAGKAPAAIKNVVTEIASTDI
                     RYTGWDFGVLPELMEIRQRGQVLVGFPALIDAGDAVTIEVFDEPELAAARHRQGLRRL
                     CALQIRDALKYLEKNVPDLQKMAVAYMQVGRTADGAGGGTAEELRQQIIDVALERAFL
                     VEPLPASADAFRQRVDEGRARLTLITGEVARLAGAILAEHAVALRKIKDTKGAPEATR
                     DAAEQLQRLMPRNFIAATPWPALQHFVRYLKAITLRLDKLRADPARDAQRMAEVRAQD
                     QRFGRLVAARKGQQDARMQELRWLLEELRVSLFAQELRTPQPVSVKRLEKVWAQMAG"
     gene            15245..15424
                     /locus_tag="PK242_03310"
     CDS             15245..15424
                     /locus_tag="PK242_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93081.1"
                     /translation="MKTRPSAPQTAPAPRNHVALALAARAGSGAAGRHQRATGAQRRA
                     DRVALQQALRRGDGH"
     gene            complement(15418..16128)
                     /locus_tag="PK242_03315"
     CDS             complement(15418..16128)
                     /locus_tag="PK242_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOZ93082.1"
                     /translation="MNRPPPPDHPPATEPIAAIVMAAGAGSRFGFRPKGLLQRDGEAL
                     LARQLRLLAQAGVSRRVVVLGHHAGDITALLRKLRAQPAQAGLSWVVNPAPDDGPGSS
                     LRCGLASLPEDLDGVLVLLGDQPLLEPQDLRAVLQAWAERAPGIELVLPLHAGQPGHP
                     LVFGPQARQAVMAAQGGAGLREWRRAHPEQVQALPVDHPRCTTDIDTEADLSTLAAAH
                     GVHLRWPAGPARDQHRNQ"
     gene            complement(16125..17165)
                     /locus_tag="PK242_03320"
     CDS             complement(16125..17165)
                     /locus_tag="PK242_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOZ93083.1"
                     /translation="MENLDVTVLRALRDWRTAGRQALLVTVVRTWGSSPRPIGSIMAL
                     CEDGAVMGSVSGGCIEDDLIARYAHPGGADALPRSGPPQFTKYGITADEAHRFGLPCG
                     GTLELLLEFDPDGAALSELVRGLDSGRLMHRRVRLADGRVRLEPADKPTELVLTDTEL
                     VNTFGPEYRMLIIGAGQMSEYLATMALFCGFAVTVCDPRDEYRSSWSVPGVRVVASMP
                     DDTVAEMQPDARTCVVALTHDPKLDDLALLQALDTPAFYIGAIGSRRNNAARHQRLIE
                     HMGQTEASLKHLRGPIGIYIGSKTPPEIAVSVMAEVLAVKNGAPLPRDLDVAYVKDQQ
                     GQTAAGSACLLP"
     gene            complement(17194..17766)
                     /locus_tag="PK242_03325"
     CDS             complement(17194..17766)
                     /locus_tag="PK242_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HOZ93084.1"
                     /translation="MEMQGSKALAITQQQAWDALNDPEVLKLCIPGCDSIEATGEGAY
                     ALVNAIKVGPVSAKFKGSLQLADVQAPDSYTIHFEGSGGAAGFGKGSAQVTLAPNATG
                     CELGYTVNATVGGKIAQVGQRLIDGVAKSMAESFFKRFEEEMQRRHPAAEAAVPAAPA
                     AAAADGAGVPLWVWILVAIVAILGVAWALR"
     gene            complement(17785..19014)
                     /locus_tag="PK242_03330"
     CDS             complement(17785..19014)
                     /locus_tag="PK242_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOZ93085.1"
                     /translation="MTRVAPDAPARRSLLGDARRGKLADNVVAFARALRRAGVPLDAA
                     RIALAQQALQCVGVERRGDVGAALEAVLVSREQDRAVFRELFDAFFRDPDLAHKLLAQ
                     MLPRAEAPAAPPPQRPRVREALQPPRPPGAAPRTQPDQSVDLDAAMTASERVRLRQAD
                     FNQLSASEYTLVERLVRDIALAVPEMRARRTRAGWRGPRVHWGRTLQGAAHSGGEVMA
                     LHRLQRRRQPLPLLLLVDVSGSMERYARLLLAFLHAATRQVQGQRVRRDVFTFGTRLT
                     DLTAAFRCSDTDAMLQQAGGAIEDFAGGTRLGDSLAVLRQRHARRLVGRRTLVLIVSD
                     GLDTGEPDVLARELAWLRRHTRRLLWLNPLLRFDGYTPSARGAAVLHRACDGMLAVHN
                     ISRLHDLARGIAQILKT"
     gene            complement(19011..19934)
                     /locus_tag="PK242_03335"
     CDS             complement(19011..19934)
                     /locus_tag="PK242_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOZ93086.1"
                     /translation="MSPTPASAASDPLSSIDALIAALQTAGYFADRRLATAVFLALRL
                     QRPLLLEGEPGVGKTELAKALAQVLGRELLRLQCYDGLEQREALYEWNYAAQLLHLRA
                     AEGRGTVEEAEREVYQPHYLIRRPLLQALQAPAPGAVLLIDEVDRADEPFEAFLLEYL
                     GEYQVSIPELGTVRAVATPLTVLTSNRTRELNDAVKRRCLYHWLDYPERERELAIVRA
                     RVPEAPAALAEQVAGFVHRLRSAPYAGHFQRAPGIAESVEWAKALVALDALLLDPEVV
                     QSTAGVLFKQREDVAALSAELITEALSPEAA"
     gene            complement(20170..20961)
                     /locus_tag="PK242_03340"
     CDS             complement(20170..20961)
                     /locus_tag="PK242_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOZ93087.1"
                     /translation="MYAFTYERPTSQADAARLAKAGGKLLAGGQTLLGSMKLRLASPE
                     QVVDLGAVKDLAGVRKDGNALVIGAMTRHADVAASADVRATIPALAQLASGIGDRQVR
                     AMGTLGGSVANNDPAADYPAAVLGLGATVVTSTRQIAADDYFQGLFATALDEGELITA
                     IRFPQPRRAAYMKFRQPASLFALIGVFVAQTDAGVRVAVTGGGNGVFRHAGLEQALSR
                     NFSPEAVAGVTIDAGELNGDLHGSAEYRAHLIGVMTQRAVAKALA"
     gene            complement(20972..>21378)
                     /locus_tag="PK242_03345"
     CDS             complement(20972..>21378)
                     /locus_tag="PK242_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOZ93088.1"
                     /translation="VRVDRFTAVDDFGVIINPMIVEGQVHGGLVQGMGQALMEHGVYD
                     QGSGQLLTGSYMDYAMPRAHDFPEFRLGHVCTPCTHNPIGSKGCGEAGAIGSPPAVIN
                     AVLDALKPLGVKDLDMPATPHRVWQAIQAAKA"
BASE COUNT         2954 a         7678 c         7567 g         3179 t
ORIGIN      
        1 cggcgcatga atcggtctcc cggggtgaca acaaaggggc aaatgtagtg catgtcaccg
       61 catggcgacg gcgcccgccc ccgagggagc gcgggcaccg gcgcgccacg gcggccgggt
      121 cgaccgacct gcggcgctca ccccacccag cgccgcgcat tgcgccagat ctgcatccag
      181 gggctgaagt cgctgcggtc gccgtcggtc cagctcatct ggatgttgcg gaacacgcgc
      241 tcggggtgcg gcatcatggc ggtgaagcgg ccgtcggcgg tggtgacggc ggtcaggccg
      301 ccgaggctgc cgttggggtt gaacgggtag cgctcggtgg gctggccgtg gtggtcgacg
      361 aagcgcatgg cggcgatcac ctggccggcg tcgccgcggc ggctgaagtt ggcgtagccc
      421 tcgccgtgcg agacggcgat cggcagccgg ctgccggcca tgccggccag caggatgctg
      481 gggctgtcca gcacttcgac catcgacagc cgcgcctcga agcgttcgct gcggttggtg
      541 gtgaagcgcg gccagtgctg ggcgccgggg atgatgtcgg ccagctcggc gaacatctgg
      601 cagccgttgc acacgcccag cccgaaggtg tcgcggcggt cgaagaaggc ctgaaactgc
      661 gccgacagcc cggcgtggaa ggtgatggag cgcgcccagc cgacgcccgc gcccagcgtg
      721 tcgccgtagc tgaagccgcc acacaccacc aggccgcgga agtcggccag ccgcacgcga
      781 ccgctttgca ggtcggtcat gtgcacgtcg tgggcctgaa agccggcttc gctgaaggcc
      841 caggccattt cgacgtgcga gttgacgccc tgctcgcgca gcaccgccac cttcggccgg
      901 gcaccgccga tgactatgtt ttttgtagct gccggcgctt gttccgactg cgccagagcc
      961 agtttttgtt tgaattcttc ggacagggcc agatgcaggc ccgggtcgcc cggcgcaccg
     1021 gcggcggcgt gttcggcgtc ggcgcagtcg gggtggtcgc gctcgcgcgc gatgcgccag
     1081 ctgacgctgt cccacacctg ctgcagatcg tgcaggctgg cgctgaacac ttcgcgcgtg
     1141 tcgcgccaca cgctcaggca gcccttgccc ttggcgatgg gcgaggcctc gggccgcgtc
     1201 ttgccgatca catggctgag ccgcgccaaa ccgtgggcgc gcagcacctg cagcacggcg
     1261 tcgcgctcgc gggtggccac ctgcagcacc acgcccagtt cctcggcgaa tagggccttg
     1321 agcgtcagca ggtcgcgccg gccgctgacc tggcgcgccc agtccttgcc ctcgcccgat
     1381 tccatgcggc tgtcgctgat gccgtcgccc tcgttgacca gcaggtcgat gttgagcgcc
     1441 acgccgacct ggccggcaaa cgccatttcg gccacgcagg ccagcaggcc gccgtcgctg
     1501 cggtcgtggt aggccaggat ccggccttcg gcgcgcagct ggttgatggc ggcgaccagg
     1561 gccttgaggt cctgcgggtc gtccaggtcg ggcacgccgt cgacctgcgg gcagccgctc
     1621 tggcccaggg tctgcgccag cacgctgccg gccaggcggt ggcggccgtg gcccaggtcg
     1681 atcaggatca ggctgctgtc gggctcatgg cggtcgagct ggggcgtgag cgtgccgcgc
     1741 acgtcggcca ggctgacaaa ggcgctggcg atcaggctga ccggcgagac caccttgcgc
     1801 gcgtgtccgt cggcgtcctg ccactgggtg cgcatcgaca ggctgtcctt gcccaccgga
     1861 atggcgatgc ccagctgcgg gcacagcgcc atgccgaccg cctgcacggc ggcgtagagg
     1921 tcggcgtcct cgcccggctc gccgcaggcg gccatccagt tggccgacag cttgacgcgc
     1981 ggcagctcga tgggcgccgc cagcaggttg gtgatggctt cggccaccgc catgcgcgcc
     2041 gacgcggggg cgtcgagcgc ggccagcggc gtgcgctcgc ccatgctcat ggcctcgccg
     2101 gcgaagccgg cgtagtcggc cagggtgacg gcgcagtcgg ccaccggcac ctgccagggg
     2161 ccgaccatct ggtcgcggtg cgtcaggccg ccgaccgtgc ggtcgccgat ggtgaccaga
     2221 aagcgcttgc tggccaccgt ggggtggctg agcacctcga tcacggcgtc ctgcagcttc
     2281 agctcatcga gctgcagggg ggtgaactgg cgctgcacgc gctgcacgtc gcgctgcatt
     2341 ttgggtggct tgccgagcag cacctccatc ggcatccgca ccggcgccgg gtcgcgatcg
     2401 tcgtcggcga cgatcagctc gcgctcgtcg gtggccacgc ccaccaccgc cagcgggcag
     2461 cgttcgcgcg cgcacagctg ctcgaacagc ggcagcgact cgggcgcgat ggccagcacg
     2521 tagcgttcct ggctttcgtt gcaccagatt tccttgggcg ccaggccgga ttcttccagc
     2581 ggcacggcgc gcagatcgaa gcgcgccccg cgcccggcgt cgttgaccaa ctccggaaag
     2641 gcgttggaca gcccgcccgc gcccacgtcg tggatcgcca gaatggggtt ggccgcgccc
     2701 agcgcctggc actgggtgat gacttcctgc gcgcggcgct ggatttcggg gttgccgcgc
     2761 tgcaccgaat cgaaatcgag ctgcgccgcg ttggcgccgc tggccagcga cgaggcggcg
     2821 ccgccgccca tgccgatgcg catgcccgga ccgcccagct ggatcagcag gctgccggcg
     2881 gggaattcga gcttgtgcgt cagccgcgcg tcgatgtgcc ccaggccgcc ggcgatcatg
     2941 atcggcttgt ggtagccgcg cagcacctgg tggccgggtg cccccacgtt cagttcgtac
     3001 tcgcgaaagt agcccagcag gttgggccgg ccgaactcgt tgttgaaggc cgccccgccc
     3061 agcgggcctt cggtcatgat ctgcagcgcg ctggccatgt gctcggggcg cccgtgcgga
     3121 ctgtcccaca gcttgctgac gctgaagccg gtcagccccg ccttgggctg ggcaccgcgg
     3181 ccggtggcgc cttcgtcgcg aatctcgccg ccggcgccgg tgctggcgcc aggaaacggg
     3241 ctgatcgccg tggggtggtt gtgggtttcc accttcatca gcacatgccg ccgcatgctt
     3301 tgtttttgat agctgccggc gcttgcctga tcggcttcga acggcgattt gacttgaaac
     3361 gcctggacct cgccgccttc catcacggcg gcgttgtcgc tgtaggccac caccgtatgc
     3421 tgcgggctgg cctggtgcgt gttccggatc atggcgaaca ggctgcgctc ctgcgccacg
     3481 ccgtcgatgc tgaactgggc gttgaagatc ttgtgccggc agtgctcgct gttggcctgg
     3541 gcgaacatca tcagctcgac gtcggtgggg ttgcgcccca ggcgggtgaa ggcgtccagc
     3601 aggtagtcga tttcgtccgc cgccagcgcc aggccccagt cgcggttggc gcgctccagc
     3661 gccgcgcggc cgccgcccag cacatcgacc tgggccagcg gctcgggcgc cagggtcgac
     3721 agcagcgcgc gcgccgcctc gcggtcgggc agcacgcttt cggtcatgcg gtcgtgcagc
     3781 agcccggcga tcgcggtgcg ctcctgctgt gatagctgct ggcgcttgat cagagccggt
     3841 ttgagccaga ttcggtattc aaccaggcgt tccagccgat gcagcgccag accgcagttg
     3901 cgggcgatgt cggtggcctt gctggcccag ggcgagaccg tgcccagccg cggcgtgacg
     3961 accaacccct cgccctgcgg ccactcgccc gcgggggcgt cgccgccgtc cagcaaggcc
     4021 gccaggcgct ccagctcggc ggcggcgggg gcggcgtcga agccggccag gtgcacgtga
     4081 cgcgcggcca cgccctccac cgccggtgcc accgcctgca ggcggggcag cagctggcga
     4141 aggcggaagt cactgagggc gttgccgccg tcgaagaagt ggatgtgcag ggtcacgtcg
     4201 ggaggcctgg gtggggcagt ggcgcgcggc gccgggtcgg ggcgactggc cgctgactgg
     4261 ggccagacgc gaagccgtcg attttatccg ccgccccggg cgcgacaggg cgcgacctgg
     4321 acggcgcagg cgcgtcgcgc gcccctctgg gataatttgc cccatgtcaa tcaccatcaa
     4381 agacgaagcc ggcatcgccg ccatgcgcga agcctgccgc ctggcggccg aggtgctgga
     4441 caccctgacg ccgctgatcc agccgggcat caccacgctc gaaatcgacc gcctggccgc
     4501 ccaggccatg gccgcccagg gcacgcgctc ggccaccgtc ggctaccagc cgcccggcta
     4561 tccgccctac ccggcgcatg tgtgcacctc ggtcaaccag gtggtgtgcc acggcatccc
     4621 ggccgacaag ccgctcaaga agggcgacat cgtcaacgtc gacgtcaccg tcatcacgcc
     4681 cgacggctgg tacggcgaca ccagccgcat gttcgagatc ggcgactgct cgatcgccgc
     4741 ccgccgcctg tgcgccctga cctaccaggc gatgtggctg ggcatcgagc aggtgcgcgc
     4801 cggcgcccgc ctgggcgaca tcggccacgc gatccagaag ttcgtcgaag gccacggcct
     4861 gtcggtggtg cgcgagttct gcggccacgg catcggccag aacttccacg aagagccgca
     4921 ggtgctgcac tacggccgcc ccggcaccgg ggtggagctg gagccgggca tgatcttcac
     4981 catcgagccc atggtcaacg tcggccggcg cgacatcaag gaactgcccg acggctggac
     5041 catcgtcacc aaggaccgca gcctgtcggc gcagtgggag cacacggtgc tggtcacgcc
     5101 caccggctac gaggtgctga gcctgtcggc cggcgcgccg gcgattcccg atttcgtcaa
     5161 gggcgtggcg cgcgccgccg cctgagcacg gatcaaccct ccgccccacc ttctgaaccc
     5221 gtcccgcatg tccgagcgcg ccgccgcctc ctgcgccgag gcgccacccg gcgactaccg
     5281 ggggcgcaag aaggcgctgc tggacgcgct gcgcgccagc ggccccggca cacgcggcgt
     5341 gcgcgccgcc ctcacccgcc tgagcgcgct ggccgacgac gcgctgcgcg cgctgtgggc
     5401 gcagcgcggc ttcgacgatg ggccggccct gctggcggtg ggcggctacg gccgcggcga
     5461 gctgttcccc tattccgaca tcgacgtgct gctgctgctg ccggacggct gcgatgcggc
     5521 gcaggacgcg gcgctgcgcg cgcgcatcga aggcttcatc accgactgct gggacgccgg
     5581 gctggaaatc ggctcggcgg tgcgcagcat cggcgactgc ctgcaggccg gcgccgaaga
     5641 cgtgacggtg cagaccgcgc tgctggaggc gcgctgcatg gccggcagcc gcgcactgtt
     5701 cgacgccttc cgccagcgcc accaggcggc gctggcgccg ctggacttct tcagcgccaa
     5761 gcggctggag ctgcgccagc gccacgtcaa gtacgaagac acgccctacg cgctggagcc
     5821 caactgcaag gaatcgcccg gcggcctgcg cgacctgcag atcatcctgt ggacggcgcg
     5881 cgccgccggc atcggcgggc gctgggacga cctgcaccgc gccggcctgg tgaccgcgca
     5941 cgagctgcgc cagctgcgcc acaacgaggc gttgctgtcc agcatccgct gtcgcctgca
     6001 cctggtggcc gggcggcgtg aagaccggct ggtgttcgac ctgcagaacg cggtggccga
     6061 atccttcggc ctgagccacc gcctggccgc cgacggccgc gtgctgctgc gcgccagcga
     6121 ggtgctgatg cggcgctact actgggccgc caaggcggtg acccagctca accagatcct
     6181 gctgcagaac ctggaagagc acctgcgcgc cgcccacggc gagccggcgc cggcgctgcg
     6241 acgcatcaac ccgcgcttct tcgaccgcga cggcctgatc gaagtcgcca gcgacgagct
     6301 ctaccagcgc cagccgcacg cggtgctgga aacctttctg ctctacaccc agacgcccgg
     6361 cgtcaagggc ctgtcggcgc gcaccctgcg cgcgctctac aacgcgcgca gcgtgatgaa
     6421 ccacgccttc cgcgccgacc cggtcaatcg ccgcaccttc ctggccatcc tgcagcagcc
     6481 gcagggcatc acccacgcca tgcggctgat gaaccagact tccgtgctgg ggcgcacgct
     6541 gtgggtattt cggcgcatcg tcgggcagat gcagcacgac ctgttccacg tctacacggt
     6601 ggaccagcac atcctgatgg tgctgcgcaa cgtgcggcgc ttcttcatgg ccgagcacgc
     6661 gcacgaatac ccgttctgct cgcagctggc ggccggctgg gacaagccct ggctgctgta
     6721 cgtggcggcg ctgttccacg acatcgccaa ggggcgcggg ggcgaccact cgcgcctggg
     6781 cgcgctggag gtgcggcgct tctgcgccga gcacggcgtg gccggcgccg acgccgaact
     6841 gctcgaattt ctggtgcgcg agcacctgag catgagccgc atcgcgcaga gcgaggacct
     6901 gagcgacccc gaggtgatcg ccgccttcgc gcgccaggtc ggcaacgaac gccagctcac
     6961 cgcgctgtac ctgctgaccg tggccgacct gcgcggcacc agccccaagg tgtggaacgc
     7021 ctggaagggc aagctgctgg aagacctgta ccgcctgacg gtgcgcagcc tgggcgggcg
     7081 agcgcccgac gccgacgccg tgatcgaggc gcgcaagcgc gaggcgctgc gcgagctcac
     7141 ccccgccggc ggcccgcccg ccctgccgcc accgctgtgg gagcagttgc aggtcagcta
     7201 cttcatgcgc cacgacgccg gcgacatcgc ctggcatgcg cgcgagctgt cgcccgccct
     7261 cgccggcagc gcggccacgg cgccagacca gccgctgcgt gccaccacgg tgcgcgcccg
     7321 cctgtcgccg gtgggcgaag ggctgcaggt gctggtgcac gccagcgacc ggccggacct
     7381 gttcgcgcgc atctgcggct ggttcgagcg cagcggcttt tccatcctgg aggccaagat
     7441 ccacaccacg cgcgacgtct gggcgctgga caccttccag atcgtgcgcc ccgaggcgcc
     7501 cgaggactac gccgagttca tcgacgccgt gcaggccgag ctgccgcccg ccatcgacag
     7561 ccaggggccg ctgccggcgc cgcgcctggg gcgcttgtcg cggcgcgtca ggagctttcc
     7621 ggtggccccg cgggtggacc tgcggcccga cgaaaagggc cagcgctggc tgctgtcggt
     7681 caccgccagc gatcgcatgg gcctgctgta tggcattgcc cgcgtgctgg cggcgcacca
     7741 gatcgacgtc gagctggccc ggatcaccac cctgggcgag cgcgtggaag acagctttct
     7801 gctgcaaggg gcccagctgc agcacgcacg cacccagctg gccatcgagc aggaactgct
     7861 ggacgtgctg gagccggcct gacaactcct gtttcgatag ctgctggcgc ttgctgcata
     7921 aggcgtggag gcgcaaaaag cacttgaccg acaccggcca cggcaccgcg cccgccgccc
     7981 cggcggccag tgcagtgttt cgcgcggtca ggcacttgta gaagcctttt tctggcttcc
     8041 cgcagggcga gccgccaagg ggccatctgc cttgttgcgt ggccttgaca tggctccacc
     8101 atgcctgcgg ccacgcgccg cgcagctggc cccttggcga ctcgctataa gtacctgaca
     8161 gcgcgaaaca ctgcactaac attacgggtt ttcccgtagc gcgctgcggc gcgccccgtt
     8221 cgattcccca tcccccgagc ctgagcatga acccgcttgc ctccccttcc cgccgcctgc
     8281 tgctggccag cgccgccgcc accgccctgc tgtggggctg cagcaagacc gaaaccccgg
     8341 ccccggccgc acccgagcct gccgcgcccg cggccgccac tgcgcctggg ctgccggcca
     8401 gaatcgtcat cggcctggac gacaacttcc cgcccatggg ttttcgcgac gacaagaacc
     8461 agctggtcgg cttcgacatc gacctggcgc gcgaggccgg caagcgcctg ggcatcgaag
     8521 tcgagttcaa gccgatcgac tggaacgcca aggaggccga actcagcggc aagcgcgtcg
     8581 acgtgctgtg gaacggcctg accatcaccg aagagcgcaa gaaaaacatc gccttcaccg
     8641 cgccctacat gcagaaccac cagatcgtcg tggtcaaggc cgacgcggcg atcaaggcca
     8701 aggccgatct ggccggcaag gtggtgggcg tgcaggacgg cagcagcgcc gtcgaggcca
     8761 tcgagaaaga cgccgccacc gcccagagct tcaaacagct caagaagtac ggcgacaacg
     8821 tgacggcgct gatggacctg tcggccggcc gcgtcgacgc cgtggtgctg gacgaggtgg
     8881 tgggccgcta ctacagcgcc aagaagccgg gcgaatacgc cgtgctggac gagcacttcg
     8941 gcaccgagga ctacggcgtc ggcctgcgcc aggacgatgc cgccctgcgc acgcggctgg
     9001 acgaggtact gacggccatg aagggcgacg gcagcggcgc caggattgcc gagcagtggt
     9061 ttggcaagaa catcttgaag tgacccaccc ccatggctcc acgcgctgcg cgatgttgcg
     9121 cctcccccct caagggggcg cagcctgtgg ccaggcaaac cccggccacg gctgccgcgg
     9181 gcatggcgtg ctccgcagcc acgcgactcg tttcgggtca accacctgac ccccaaaaac
     9241 cgcaccagca tcggtgatgg actacctgct gcgcatcctg ccaccgctgc tcgaaggcgc
     9301 cggcgtcacg ctgggcctgt tcgtggtcac gctggcgctg tcgctgccgc tcggcctggc
     9361 gctggcgctg gcgcggctgt cgcgctgggg cgcgctgcgc cgcgccgtca gcggcttcgt
     9421 ctggctgatg cgcggcacgc cgctgatgct gcagctgctg ttcgtctact acgccctgcc
     9481 cttcgtgccg gtgatcggcg tgcgcctgcc cgactacccg gcggcggtgg tcgccttcac
     9541 gctcaactac gcggcctact ttgccgaagt cctgcgcgcc ggcatccagt cgatcgaccg
     9601 cggccagtac gaaggcgcca aggcgctggg catgagctac gcccagacca tgcgccgcat
     9661 cgtcatgccc caggtgtggc gcggcagcct gccgccgctg gccaacgaga ccatcaccct
     9721 ggtcaaggac acctcgctga tctacgtgct ggcgctcaac gacctgctgc gcgccgcgcg
     9781 cggcatcgtg cagcgcgact tcagcatcac gcccttcgtg gtcgccgccg ccttctacct
     9841 gctgatgacg ctggtgctga cctggatctt tcagcgcctg gagcgccgcc acgccgccca
     9901 tgtcgcctga acaacgcccc cccatggtgc gcgccagcgg cctgcacaag cactttggcg
     9961 cgctgcacgt gctgcgcggc gtgtcgctga cggtcgagcg cggcgaggtg gtggccatcc
    10021 tggggccgtc gggctcgggc aaatcgaccc tgctgcgctg cttgaaccac ctcgaaacga
    10081 tcgagcgcgg tggcatcgag atcgcaggcg aaacgctcgc cagcgccgat gcggcgggcg
    10141 ccagcagcta tccttctgat agcgaagtgc gccgcatctg ccagcgcacc ggcatggtgt
    10201 tccagcattt caacctgttc ccgcacctga gcgtgctggg caacctgatc gaggcgccga
    10261 tcacggtgcg caagctggcg cgcgccgagg tcgtgccgcg cgccgaggcg ctgctggcca
    10321 aggtgggtct ggccgacaag cgcgacgtct accccggccg gttgtcgggc ggccagaagc
    10381 agcgcgtggc gatcgcgcgg gcgctggcca tgcagcccga catcctgctg ttcgacgagc
    10441 cgaccagcgc gctcgacccc gaactcaccg gcgaggtgct gcgcaccatg cgccagctgg
    10501 ccgaagagcg catgaccatg ctggtggtca cgcacgagat ggcgtttgcg cgcgaggtgg
    10561 cgcaccgcgt ggtgttcatg gacgccggcc agatcgtcga agaccggcca gcggccgact
    10621 tcttcgccgc gccccgacac gagcgcgccc gcgccttcct ggcccacatg ctgtgagccg
    10681 gcggcgtcct gaggtggccc cgttcaacct gccatctgcg cccacacctt ttccagccgc
    10741 ttgacgctga ccggctgcgg cgtgcgcagc tcctgcgcga acaggctcac gcgcagctcc
    10801 tcgagcagcc aacgcaactc ctgcatgcgc gcatcctgct ggcccttgcg ggcggccacc
    10861 aggcgcccga agcgctggtc ctgcgcccgc acctcggcca tgcgctgggc gtcgcgcgcc
    10921 gggtcggcgc gcagcttgtc caggcgcaag gtgatggcct tcaggtagcg cacgaagtgc
    10981 tgcaacgccg gccagggggt ggcggcgatg aagttcctgg gcatcagccg ctgcagctgc
    11041 tcggccgcgt cgcgcgtcgc ctcgggcgcg cccttggtgt ccttgatctt gcgcagcgcc
    11101 acggcgtgct cggccaggat ggcgccagcc agtcgcgcca cttcgccggt gatcagggtc
    11161 aggcgcgcgc ggccttcgtc gacgcgctgg cggaacgcat cggcactggc gggcagcggc
    11221 tcgaccagaa aggcgcgctc cagcgccacg tcgatgatct gctggcgcag ctcctcggcg
    11281 gtgccgccac cggcgccgtc ggccgtgcgg ccgacctgca tgtaggcgac cgccatcttc
    11341 tgcagatcgg ggacgttctt ttccaggtac ttcagggcgt cgcggatctg caatgcgcac
    11401 aggcggcgca ggccctggcg gtggcgcgcg gcggccagct cgggctcgtc gaacacctcg
    11461 atggtgacgg cgtcgccggc gtcgatgagc gccggaaacc ccaccagcac ctgaccgcgc
    11521 tgacggatct ccatcagctc gggcagcacg ccgaagtccc agccggtgta gcggatgtcg
    11581 gtggaggcga tctcggttac tacgttcttg atagctgctg gcgctttccc agcctgcgct
    11641 ggagcctgat tttgctctga atcctgcggg cctggcgcgg ccgccggcgc ggccccgggg
    11701 acggcgccct tgagcgccgc cagggcctga aaggcgccgc gcgcctcgcc gcccagctcg
    11761 gccttcagcg ccgccagatg gcgtcccatg ccgcgctggc ggccgtgctc gtcgaccacg
    11821 cggaagttca tgaacaggtg cgccggcagc atgtcgagct tgaagtcgct gcgcttgaca
    11881 tccaggctgg tggcctcgcg cacgcgcctg agcagcgcgt cggtcaggct gccgtggccg
    11941 aacacctcgg gcgcgcccag gatctcggcc agccgggcgg cgctgtcggg cagcggcacg
    12001 aagcggctgc gcggcttttg cggcaggctt ttcagcagcg cctggatctt gtccttcagc
    12061 atgccgggca ccagccattc gcagcgcgcc tcgctcacct ggttgagcac gaacagcggc
    12121 accgtcaccg tcaggccgtc cctggcgtcg cccggttggt gcaggtagct ggcggcgcag
    12181 tccacgccgc ccaggcgcac catcttggga aaggcgctgg tggtgatgcc ggcggcctcg
    12241 tggcgcatca gctcctcgcg cgtgagaagc agcaggttgg gtttggcgcg gctggcttcg
    12301 cgccaccagc gctcgaagtc gcgcccgttg cagatgtcgg ccgggacctg gctgtcgtag
    12361 aaggcgtaga tcagctcctc gtcgaccagc acgtcctggc ggcggctctt gtgctccagc
    12421 tcctcgacct tggcgatggt gcgctggttg gccagcagaa agggcaggcg ccgcgcccac
    12481 tcctcgggcc agtcgccgcc caccagcgcc gcgcggatga agatctcgcg cgcggcgcgc
    12541 ggatcgaccg caccgaagct gcggcgccgg ccgccgtaga ccagcaggcc atagagcgtg
    12601 gcgcgctcca gcgccaccac gtcctgcgcc ttcttttccc agtgcggctc cagcagctgc
    12661 tttttcagca gatggccgcc gacctcttcc agccactgcg gctcgatggc ggcgatgccg
    12721 cggccgaaca ggcgggtggt ttccaccagc tcggcgcaga cgatccactt gcccggccgc
    12781 ttgctcaggt gcgcgcccgg atggcggtgg aacttgatgc cgcgcgcgcc caggtattcg
    12841 ccgctctggg cgtcgtcgcc ctccagcttg cagccgacgt tgcccagcag ccccgccagc
    12901 agcgatttgt gcagcgcctc gtagcccgaa ggttggtcgt tttcgcgcca cttgtgctcg
    12961 gccaccacgg tgtgcaactg ggtatgaatg tcgcgccact cgcgcacccg gcgcacgctg
    13021 atgaagttct ggcgcagcag ttgctcccac tggcggttgc tcagcttgtg caccgccgac
    13081 gcttcggcgt tctgaatcaa atcggaatca agcccttgtc cagaaagcgc cggaagctcc
    13141 ttttttgata gtgtctggcg tgcttgggca agggccgctg ccgcgcgctg ggccactggc
    13201 agcgcggccg cattggcggg tttgcgcgcg gccgtctgcc cttcgctggc tgccatctgc
    13261 gcgcgggtca taggcagtga ccggccggcg ccgggccgcc ccaagccggc cgcggccccc
    13321 ctggggggca gcgcagcagc gaagcgcgaa gcgtgggggc ctgttccacc gcgcgcgtcg
    13381 tgcagccact tccacagccg caggtagccg gaaaactcgc tcttctcgtc gtcgaacttg
    13441 gcgtgcgcct gatcggcctg ggcctgctgc tccagcggcc ggtcgcgcac gtcgggcacg
    13501 ctcagcgcgg cggcgatcac cagcacctcg cgcagcgcgc cgcgcgcgcg tgcctccaga
    13561 atcatgcggc cgacgcgcgg gtccagtggc aggcgcgcca gctccgtgcc cagcgcggtg
    13621 agctgctgcg cctcgtccag cgcgcccagc tcctgcagca gttgcacgcc gtcggcgatg
    13681 gcccggcccg agggcatgtc gaggaagggg aagtcctcca cccgcccgcc ctcgccgccc
    13741 aggcgcaggc tcttcatgcg caggatcacg gccgccagtg aggagcgcag gatctccggg
    13801 tcggtgaaac gcgggcggcc ggcgaagtcc tgctcgtcgt acaggcggat gcagatgccg
    13861 tcggccacgc gcccgcagcg gccggcgcgc tggcccgccg ccgcctggct gatcggctcg
    13921 accagcaact gctccacctt gctgcgataa ctgtagcgct tgacgcgcgc cgtgccgggg
    13981 tcgatcacgt agcggatacc cggcaccgtg agcgaggtct cggccacgtt ggtggccagc
    14041 acgatgcgcg gcgcgccgtg cggctcgaac acgcggtcct gctcggcctg cgacagccgc
    14101 gcgaacagcg gcagcacctc ggcgccgcgc gtcagcggcc ggtgcgacag gtgcgcgcgc
    14161 aggtggtcgg cggcttcgcg gatctcgcgc tcgccgggca gaaacaccag gatgtcccct
    14221 tgcggagggc ccacccaaag ctcatccacc gcagcggcga tggcgtcgtt caggtcgtgt
    14281 tcgcggcttt cctcgaacgg gcgccagcgc tgctccaccg ggtacatgcg ccccgacacc
    14341 tgcagcaccg gcgcagggac aaggccccca cgctccccca cttcgtgtgg gtcgctgccc
    14401 cccgaggggg ccacgcccgg cttggggcgg cccggcgccg ggacaaggcc cccacgctcc
    14461 cccacttcgt gtgggtcgct gccccccgag ggggccacgc ccggcttggg gcggcccggc
    14521 gccgggacaa ggcccccacg ctcccccact tcgtgtgggt cgctgccccc cgagggggcc
    14581 acgcccggct tggggcggcc cggcgccggg cggggcatcg cgaaatgccg ggcaaagcgg
    14641 tcggcgtcga tggtggccga ggtgacgatc agcttcaaat cgggccttcg cggcaggatc
    14701 tgtttgatgt agcccagcag gaagtcgatg ttcaggctgc gctcgtgcgc ctcgtcgatg
    14761 atcagcgtgt cgtaggcgtt gagcagcggg tcggtctgcg tctcggccag cagaatgccg
    14821 tcggtcatca gcttgacgct ggcgcccggc gtcaggcggt cgccaaagcg caccttgtaa
    14881 cccaccacct cgcccagcgg cgtgcgcagt tcttcggcaa tgcgcttggc gacactggtg
    14941 gcggcgatgc ggcgcggctg ggtgtgaccg atcagcttgc cgcgctcgcc cggccgcgcg
    15001 ttcagccgcc cgcgccccat ggccagggcg atcttgggca gctgcgtggt cttgcccgag
    15061 ccggtttcgc cgcacacgat gaccacctgg tgcgcgcgca gcgcggcctc gatctcggcg
    15121 cggcgcgccg acaccggcag gctgtcgggg aaatcgatgg tcaggcgggg tggcgcgttg
    15181 ctcatggggc tggggattat ccgcgtgcgg aactccgggc gccggatgga gtcatgttcc
    15241 tgccatgaaa acccgtccgt ccgcacccca gaccgccccg gcgccgcgca accacgttgc
    15301 gctggcgctg gccgcgcgcg ccggcagcgg cgcggccggc cggcaccagc gcgccaccgg
    15361 cgcgcagcgc cgcgccgacc gggtggcgct gcagcaggcg ctgcgccggg gcgacggtca
    15421 ctgattcctg tgctgatcgc gcgcggggcc ggcgggccag cgcaggtgca ccccgtgcgc
    15481 ggccgccagc gtggagaggt cggcctcggt gtcgatgtcg gtggtgcagc gtgggtgatc
    15541 gacgggcagc gcctgcacct gctcggggtg cgcgcggcgc cattcgcgca ggccggcgcc
    15601 gccttgcgcc gccatcacgg cctggcgcgc ctgcgggccg aacaccagcg gatggcccgg
    15661 ctggccggcg tgcagcggca gcaccagctc gatgccgggc gcccgctcgg cccaggcctg
    15721 cagcacggcg cgcaggtcct gcggctccag cagcggctga tcgcccagca gcaccagcac
    15781 gccgtccaga tcttcgggca ggctggccag gccgcagcgc agggaagaac cggggccgtc
    15841 gtcgggcgcg gggttgacga cccagctcag cccggcctgc gccggctgtg cgcgcagctt
    15901 ccgcagcagg gccgtgatgt cgcccgcgtg atgacccagc acgaccaccc gacgcgacac
    15961 cccggcctgc gccagcaggc gcagctgacg cgccagcagc gcttcgccgt cacgttgcag
    16021 caggcccttg ggcctgaagc caaaacggct tccggcgccc gccgccatga cgatggcagc
    16081 tatcggttcg gtagcaggcg ggtgatccgg cggcggcggt cggttcacgg aagcaggcag
    16141 gccgagccgg ccgccgtctg gccctgctgg tccttgacgt aggccacgtc caggtcgcgc
    16201 ggcagcggcg cgccgttctt cacggccagc acctcggcca tcacgctgac ggcgatctcg
    16261 ggcggcgtct tgctgccgat gtagatgccg atcgggccgc gcaggtgctt gagcgaggcc
    16321 tcggtctgcc ccatgtgctc gatcagccgc tggtggcggg cggcgttgtt gcgccggctg
    16381 ccgatggcgc cgatgtagaa cgccggcgtg tccagcgcct gcagcagcgc cagatcgtcc
    16441 agcttggggt cgtgcgtgag cgccaccacg caggtgcgcg cgtcgggctg catctcggcc
    16501 acggtgtcgt cgggcatgct ggccaccacg cgcacgccgg gcacgctcca gctgctgcgg
    16561 tattcgtcgc gcgggtcgca cacggtgacg gcaaagccgc agaacagcgc catggtggcc
    16621 aggtattcgc tcatctggcc ggcgccgatg atcagcatgc ggtactcggg gccgaaggtg
    16681 ttgaccagct cggtgtcggt cagcaccagt tcggtcggct tgtcggccgg ctccagcctc
    16741 accctgccgt cggccaggcg cacccggcgg tgcatcaggc ggccgctgtc caggccacgg
    16801 accagctcgc tcagcgccgc gccatcgggg tcgaactcca gcagcagctc cagcgtgcca
    16861 ccgcacggca gaccgaagcg gtgcgcttcg tccgcggtga tgccgtactt ggtgaactgc
    16921 ggcgggccgc tgcgcggcag cgcgtcggcg ccgcccgggt gggcgtagcg cgcgatcagg
    16981 tcgtcctcga tgcagccgcc cgagaccgag cccatgaccg cgccgtcctc gcacaaggcc
    17041 atgatcgagc cgatcggccg tggcgacgag ccccaggtgc gcaccacggt gaccagcagg
    17101 gcctgccggc ccgccgtccg ccagtcgcgc agggcgcgca gcacggtgac atccaggttt
    17161 tccatgcgac acctcctgtg gccagtgacc cggtcagcgc agcgcccagg ccacacccag
    17221 tatggccaca atcgccacca gaatccagac ccacagcggc acgccggcgc cgtcggccgc
    17281 cgccgccgcg ggcgcggcgg gcaccgccgc ctcggccgcc gggtggcggc gctgcatctc
    17341 ctcttcgaag cgcttgaaga agctctcggc catcgacttg gccacgccgt cgatcagccg
    17401 ctggccgacc tgggcgatct tgccgccgac ggtggcgttg acggtgtagc caagctcgca
    17461 gccggtggcg ttgggcgcca gggtgacctg ggcgctgccc ttgccgaagc cggcggcacc
    17521 gccgctgcct tcgaaatgga tggtgtagct gtcgggcgcc tgcacatcgg ccagttgcag
    17581 cgagcccttg aacttggccg acaccgggcc gaccttgata gcgttgacca gcgcataggc
    17641 gccctcgccg gtggcctcga tgctgtcgca gccggggatg cacagtttca gcacctcggg
    17701 gtcgttgagc gcgtcccagg cctgttgctg ggtgatggcc agggccttgc tgccttgcat
    17761 ttccatgggg tgggtccttt cctttcaagt cttcaggatc tgggcgatgc cgcgcgccag
    17821 atcgtgcagg cggctgatgt tgtgcaccgc cagcatgccg tcgcaggcgc ggtgcagcac
    17881 ggcggcaccg cgcgcgctgg gggtgtagcc gtcgaagcgc agcagcgggt tgagccacag
    17941 caggcggcgc gtgtggcgcc tgagccaggc cagctcgcgc gccagcacgt ccggctcgcc
    18001 ggtgtccagg ccatcgctga cgatcagcac caaggtgcgc cgcccgacca ggcgccgcgc
    18061 atggcgctgg cgcagcacgg ccagcgaatc gcccaggcgc gtgccgccgg cgaagtcctc
    18121 gatggcgccg ccagcctgct gcagcatggc gtcggtgtcg ctgcaccgga aggccgccgt
    18181 caggtcggtc aaccgggtgc cgaaggtgaa cacgtcgcgc cgcacgcgct gcccctgcac
    18241 ctggcgcgtg gcggcgtgca gaaaggccag cagcaggcgc gcgtagcgct ccatcgaacc
    18301 cgacacgtcg accagcagca gcagcggcag cggctggcgg cggcgctgca ggcgatgcag
    18361 cgccatcact tcgccgccgc tgtgggcggc gccctgcagc gtgcggcccc agtgcacgcg
    18421 cggaccgcgc cagccggcgc gcgtgcgccg ggcgcgcatc tcgggcacgg ccagtgcgat
    18481 gtcgcgcacc agccgttcga ccagggtgta ttcgctggcg ctgagctggt tgaagtcggc
    18541 ctgccgcagg cgcacccgct cgctggccgt catagcggcg tcgagatcga cgctctggtc
    18601 gggctgggtg cgcggcgccg cccctggcgg cctgggcggc tgcagggcct cgcgcacgcg
    18661 cgggcgctgg ggcggcggcg ccgccggcgc ctcggcgcgc ggcagcatct gggccagcag
    18721 cttgtgggcc aggtcggggt cacgaaagaa ggcgtcgaac agctcgcgga acacggcgcg
    18781 gtcctgctcg cggctgacca gcacggcctc cagcgccgca cctacgtcgc cgcgccgctc
    18841 cacccccacg cattgcagcg cctgctgcgc cagcgcgatg cgcgcggcgt cgagtggcac
    18901 gccggcgcgg cgcagcgcgc gcgcaaaggc caccacgttg tcggccagct tgccgcggcg
    18961 cgcgtcgccc agcagcgaac ggcgggccgg cgcgtccggc gccacgcggg tcacgcggcc
    19021 tccggcgaca gcgcctcggt gatcagctcg gcagacagcg ccgccacgtc ctcgcgctgc
    19081 ttgaacagca cgccggcggt gctctgcacc acctcgggat cgagcagcag cgcgtccagc
    19141 gccaccagcg ccttggccca ttccacgctt tcggcgatgc cgggggcgcg ctggaagtgg
    19201 cccgcgtagg gggcgctgcg cagccggtgc acgaagccgg ccacctgttc ggccagcgcc
    19261 gccggtgcct cgggcacgcg ggcgcgcacg atggccagct cacgttcgcg ctcggggtag
    19321 tcgagccagt ggtacaggca gcgacgcttg acggcgtcgt tgagctcgcg cgtgcggttg
    19381 ctggtgagca cggtcagcgg cgtggcgacg gcgcgcacgg tgccgagttc ggggatgctg
    19441 acctggtact cgcccaggta ttcgagcagg aaggcttcga acggctcgtc ggcgcgatcg
    19501 acctcgtcga tcagcagcac ggcaccgggc gccggtgcct gcagcgcctg cagcagcggg
    19561 cggcggatca ggtagtgggg ctggtagacc tcgcgctcgg cctcctccac cgtgccgcgc
    19621 ccttccgccg cacgcaggtg cagcagttgc gcggcgtagt tccattcgta cagggcttcg
    19681 cgctgctcca ggccgtcgta gcactgcagg cgcagcagct cgcgccccag cacctgggcc
    19741 agggccttgg ccagctcggt cttgcccacg cccggctcgc cttcgagcaa cagcgggcgc
    19801 tggagcctga gcgccagaaa caccgccgtg gccaggcgcc ggtcggcaaa gtagcccgcc
    19861 gtctgcagcg cggcgatgag ggcgtcgatg gacgacagcg ggtcggacgc ggcggacgcc
    19921 ggggtgggtg acatgaagct tccgaaggtg aatggaagat actacgaatt cgatagctgc
    19981 ttgcgcttgc tggatgcgcg ccggagcaac tttttttcat gaagtccggc gtgccgctgc
    20041 ggccccgggg cgaggcctgt tcgcacaggg gcgcggctgg cgaccggcgg cagcagctgc
    20101 cgtgctgccg cccgcccggc gtcgccgcct tccgcctgac cgcgccaggc gccgcagccg
    20161 cacccagggt caggccagcg ccttggcgac ggcgcgctgc gtcatgacgc ctatcaggtg
    20221 cgcgcggtat tcggcgctgc cgtgcaggtc gccgttgagc tcgccggcat cgatcgtgac
    20281 gccggccacc gcctcggggc tgaagttgcg gctgagcgcc tgctccagtc cggcgtggcg
    20341 gaacacgccg ttgccgccgc cggtcacggc cacgcgcacg ccggcgtcgg tctgcgccac
    20401 gaacacgccg atcagcgcga acagcgaggc cggctgacgg aacttcatgt aggccgcgcg
    20461 acgcggctgc ggaaagcgaa tggcggtgat cagctcgccc tcgtccagcg cggtggcgaa
    20521 caggccctgg aagtagtcgt cggcggcgat ctggcgcgtg ctggtgacca cggtggcgcc
    20581 caggccgagc acggccgccg ggtagtcggc cgccgggtcg ttgttggcca ccgatccgcc
    20641 cagcgtgccc atggcgcgca cctggcggtc gccaatgccg ctggccagct gcgccagcgc
    20701 cggtatggtg gcgcgcacgt cggcgctcgc ggccacgtcg gcgtggcgcg tcatggcgcc
    20761 gatgaccagc gcgttgccgt ccttgcgcac gccggccaga tccttgacgg cgcccaggtc
    20821 caccacctgc tcgggggaag ccaggcgcag tttcatcgat cccagcaggg tctgcccgcc
    20881 ggccagcagc ttgccgcccg ccttggccag cctggcggcg tcggcctggc tggtgggacg
    20941 ctcgtaggtg aatgcgtaca tgtgtgtctc ctcaggcttt ggccgcttga atcgcctgcc
    21001 acacgcggtg cggcgtggcg ggcatgtcca gatccttcac gcccaggggt ttgagcgcgt
    21061 ccagcacggc gttgatcacc gccggcggcg agccgatggc gcccgcttcg ccgcagccct
    21121 tgctgccgat cgggttgtgc gtgcagggcg tgcagacatg gccgagccgg aactcgggga
    21181 agtcgtgcgc gcgcggcatg gcgtagtcca tgtagctgcc ggtgagcagc tggccgctgc
    21241 cttggtcgta cacgccgtgc tccatcaggg cctgacccat gccctgcacc agaccgccgt
    21301 gcacctggcc ttcgacgatc atcgggttga tgatgacgcc gaagtcgtcc accgcggtga
    21361 agcggtccac ccgcacca
//
LOCUS       DAOOOG010000023        21373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_2407_length_21373_cov_24.312834, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000023 DAOOOG010000000
VERSION     DAOOOG010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 21373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21373
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2407_length_21373_cov_24.312834"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..111)
                     /locus_tag="PK242_03350"
     tRNA            complement(38..111)
                     /locus_tag="PK242_03350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77..79),aa:Gly,seq:ccc)
     gene            complement(155..655)
                     /locus_tag="PK242_03355"
                     /pseudo
     CDS             complement(155..655)
                     /locus_tag="PK242_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            complement(658..1409)
                     /locus_tag="PK242_03360"
                     /pseudo
     CDS             complement(658..1409)
                     /locus_tag="PK242_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
     gene            complement(1486..2823)
                     /gene="hpnE"
                     /locus_tag="PK242_03365"
     CDS             complement(1486..2823)
                     /gene="hpnE"
                     /locus_tag="PK242_03365"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HOZ93089.1"
                     /translation="MKRADGSALCVAIVGAGWAGMAAAVRAVELGHDVTVFEAARQLG
                     GRARGLPLTLPDGRELMVDNGQHILIGAYIDCLRLMRSLGVDPGQALLRRPLTLMYPD
                     GSGLALPDWPAPLDAAAGILAARGWSWRDKRALLAAALGWRRAGFVCPPQLSVADLCR
                     ALPPRLRAEFIEPLCVSALNTPMAEASAQVFLRVLRDALFGVRGGSNLLLPRVDLGAL
                     LPQAAARWLQARGARVLTGQRVAALQWQPGGWAIDGQAFDQVILATSAPHAAQVGMAT
                     APSAPDSIADSLRAWAAGTQGLAHRAITTVYAQAAPPAAGARLLRAPMLALASGPSAP
                     AQFVFERDAIAPPADAPTGLLAFVVSNSAGERDALQAALLAQAQAQLGLALTPLLCVT
                     ERRATFACTPGLQRPLARIAPGLLAAGDYIEGPYPATLEGAVRSGLHAAQAIG"
     gene            complement(3040..3894)
                     /gene="hpnD"
                     /locus_tag="PK242_03370"
     CDS             complement(3040..3894)
                     /gene="hpnD"
                     /locus_tag="PK242_03370"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HOZ93090.1"
                     /translation="MTAPPQSPQDYVQQKAAASGSSFYYAFLFLPPPRRAAITAFYAF
                     CREVDDVVDEVSDPGVAATKLAWWQSEVGRAFGGQPTHPALQALMPHAAAFGIESRQL
                     LAVIEGCQMDLTQTRYLDFAQLQRYCHLVAGVVGEVSARIFGQQDQRTTAYAHRLGLA
                     FQLTNILRDVGEDARRGRIYLPVDELQRFEVKAHEILNGQHSDRFERLMRFQAERAHR
                     LYDEALALLPAADRRSQKPGLMMASIYRTLLREIEADGFQVLRQRIALTPLRKLWLAW
                     KMQALGRF"
     gene            complement(3971..4861)
                     /gene="hpnC"
                     /locus_tag="PK242_03375"
     CDS             complement(3971..4861)
                     /gene="hpnC"
                     /locus_tag="PK242_03375"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HOZ93091.1"
                     /translation="MSARPSPPADAASALPAAVAHYENFPVASWLCPPRLRPPIGAIY
                     HFARTADDIADEGEAAPARRLADLAAYRADLHAVAAGRSPSARWPQVFGPLAAQMRAH
                     ALPLQPLDDLLSAFAQDVAFTRDGAAYADMAELLDYCRRSANPVGRLLLHLYGVRDER
                     ALAESDAICSALQLINFWQDLSVDIPRGRYYLPLADCAAHGVRIRQPARDFAALRSDP
                     NTINLIATCANHARATMQKGANLVHRVPGRAGWELRAVVQGGLRILDKIAAQGFDTLA
                     RRPTVGKADAPLIAWRMLWM"
     gene            4967..6211
                     /locus_tag="PK242_03380"
     CDS             4967..6211
                     /locus_tag="PK242_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ93092.1"
                     /translation="MLTNRPLAPAGAPAAATVSRRRSTGAVGAALLAGLCATLLAGCG
                     KAPPAAEPVRAVKLITVAPAGLQTAPEYVGEVRARVESRLGFRVAGKITRRAVDVGQR
                     VAAGQLLAQLDARDYQLAADAARAQLSAATTQRDLAAADLKRFQGLREQGFVSAAEIE
                     RRSAQLQAAQATLEQARAQLNAQGNQTGYTQLLADAPGVVTAVEAEVGQVVAAGTPVV
                     RIAQDGARDAVFAVPEDRVAAVRVGQPVGVRAWAGGSQWPGTVREVAASADPVTRTFA
                     VKVALAGDPPPLGATVYVAPAADERVAAGVIKLPTSALRHDGNGSAVWLYDAASGSVR
                     LQPVQLAGPDGNEVVVASGLTPGMQVVATGVHVLTPGQKVSVYVPNVAPAGAEVGSHA
                     MKPIANGAGGGAAASAAPAASR"
     gene            6228..9485
                     /locus_tag="PK242_03385"
     CDS             6228..9485
                     /locus_tag="PK242_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ93093.1"
                     /translation="MPHQSPIDGAADAAATPAKPFNISRWALEHSALTRYLMVVLMLL
                     GVAAYFQLGQDEDPPFTFRVMVVRAFWPGATARQVADQVADKIERTLQEVPYADKIRS
                     YSKPGEALIIFQLKDSSPPRDVPQLWYTVRKKVGDMRQTLPAGVQGPFFNDDFGDVYG
                     VIYALSGEGFSPAELKTFADDARQRLLRVKDVAKVELFGVQDEKVHVEISQKRLAQLG
                     LDMGQVLGQLGSQNAIESAGAVQTPLDVVQVRVAGQFNDLEQLRAMPIRAPNGRQLRL
                     GDIASVTRGYVDPPQVKVRHDGREVIALGVSMAKGGDIIALGKGLREASAQIERTLPL
                     GVSLAQIQDQPGAVASSINEFVKVLIEAVVIVLAVSFLSLGLHKRPGQHPLWRRWTLD
                     VRPGLVVGITIPLVLAVTFLAMHYWGIGLHKISLGSLIIALGLLVDDAIIAVEMMVRK
                     LEEGYDKLRAATFAYEITAIPMLTGTLITAVGFLPIGMAKSTTGEYTFAIFAVTVVAL
                     LVSWVASVVFVPYLGAWLLRLPPHVQAARAEALNRGVPVTEEPHELFDTPFYNRFRAA
                     VSWCVAHRWLTIGATVLVFGLGLFGMTRVQQQFFPDSVRPEILVDLWAPEGTAFVATE
                     EVARRVEARLRGEPGVRGVTAWVGSGVPRFYLPLDQIFPQSNVAQLIVVAQDLAERNR
                     LRLDLAALLAREFPEVRARVKFLPNGPPVAYPVQFRVLGPDPATLRARADEVRAALQA
                     HPNTRGVNDNWNESVKVVRLEIDQAKARALGVSSQSIAQAGRTLLAGSTIGQFREGDK
                     LIDIVLRQPLAERDAITDLAYTYLPTASGQSIPLTQIARPVFDWEPGVLWRENRDYAI
                     TVNADVAEGLQGATVTFALQPELQAMQARWHAAGQSAYRIEVAGAVEESSKGSSSIAA
                     GIPAMLFITFTLLMLQLHSFSRALLVFITGPLGIAGVAAALLLLNRPFGFVALLGVIA
                     LMGMIQRNSVILIDQIEKDRERGVPAWDAIVEAAVRRLRPIVLTAAAAVLAMVPLSRS
                     VFWGPMAVAIMGGLIVATALTLLALPAMYAAWFRVRPPARENPAPAPVSGRSSAS"
     gene            9557..9643
                     /locus_tag="PK242_03390"
     tRNA            9557..9643
                     /locus_tag="PK242_03390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9591..9593,aa:Leu,seq:tag)
     gene            9673..10986
                     /gene="tig"
                     /locus_tag="PK242_03395"
     CDS             9673..10986
                     /gene="tig"
                     /locus_tag="PK242_03395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOZ93094.1"
                     /translation="MAVNVETLEKLGRKMTLTLPVETIQSEVDARLRKLARTVKMDGF
                     RPGKVPLSVVARQYGYSVQYEVMNDKVGEAFFNAANEANLRVAGQPTITEKDGAPEGQ
                     MAFDAVFEVFPDVKIGDLSVAEIEKLSADVGDEAIDRTLEILRKQRRSFAQRAKDAPA
                     EDGDRVTVDFAGKIDGEPFEGGKAEDFQFVIAEGQMLKEFEDAVRGMKVGESKTFPLA
                     FPADYHGKDVAGKTADFLVTVNKIEAAHLPEVNEQLAKSLGVKDGTVEGLRADIRKNL
                     EREVKFRLNQRNKQAVMDALVAKAELDIPNSSVQAELDRMVQSARADLKARGIKDVEK
                     LPIPEDGFRPQAEQRVRLGLVLAELVKANDLQAKPEQIRAQVEELAASYEKPEEVVRY
                     YYADNRRLADVEAMVIENNVTEFVLGRAKVTEKKLGFDELMGQQG"
     gene            11155..11766
                     /gene="clpP"
                     /locus_tag="PK242_03400"
     CDS             11155..11766
                     /gene="clpP"
                     /locus_tag="PK242_03400"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOZ93095.1"
                     /translation="MSQYDTPSNLGLIPMVIEQSGRGERSYDIYSRLLRERVVFLVGE
                     VNDQTANLVVAQLLFLESENPDKDISLYINSPGGSVTAGMSIYDTMQFIKPDVSTMCL
                     GFAASMGAFLLAAGAKNKRYSLPNSKVMIHQVLGGARGQATDIEIHARDILRTKDQMN
                     RILAERTGQPLEKIQRDTERDYFMTADEAKDYGIVDQVIANRP"
     gene            11954..13222
                     /gene="clpX"
                     /locus_tag="PK242_03405"
     CDS             11954..13222
                     /gene="clpX"
                     /locus_tag="PK242_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOZ93096.1"
                     /translation="MADKKGSSSSEKTLYCSFCGKSQHEVKKLIAGPSVFICDECIDL
                     CNDIIREEVPEGAAEGEARSDLPTPADIKANLDNYVIGQEKAKRTLSVAVYNHYKRLK
                     HKEDAKGGDVELAKSNILLIGPTGSGKTLLAQTLARQLDVPFVMADATTLTEAGYVGE
                     DVENIISKLLQSCNYDVERAQRGIVYIDEIDKISRKADNPSITRDVSGEGVQQALLKL
                     IEGTMASIPPQGGRKHPNQDFLQIDTTNILFICGGAFAGLEKVIEARTEASGIGFGAT
                     VRSKAQRSITEVFQEVEPEDLIKYGIIPELVGRMPVVATLAELTEDALVQILTEPKNA
                     LVKQFGRLLQMEGVELEIRPSALKAIARKAIARKTGARGLRSIMEQALIDTMFELPHT
                     ANVAKVVVDESTIEDAQPPLLVYREAAKKA"
     gene            13481..15916
                     /gene="lon"
                     /locus_tag="PK242_03410"
     CDS             13481..15916
                     /gene="lon"
                     /locus_tag="PK242_03410"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOZ93097.1"
                     /translation="MSGTTPLPATPIDLPLLPLRDVVVFPHMVIPLFVGRPKSIKALE
                     TAMAADRRIMLVAQKTAAKDEPSVTDMFEVGCVSTILQMLKLPDGTVKVLVEGQQRAT
                     VTHIEDGEAHFVATVTPLDGEAADKDNAEVEALRRAVMQQFDQYVKLNKKIPPEILTS
                     IASIDEPGRLADTIAAHLPLKLENKQLVLDLASVKDRLDNLFEQLEREVDILNVDKKI
                     RGRVKRQMEKNQRDFYLNEQVKAIQKELGEGEEGADIEEIEKKIKAARMPKEARKKAD
                     AELKKLKLMSPMSAEATVVRNYIDVLVGLPWAKKTKIKHDLGYAEQVLNEDHYGLEKV
                     KDRILEYLAVQQRVDKVKAPILCLVGPPGVGKTSLGQSVAKATGRKYVRMALGGMRDE
                     AEIRGHRRTYIGAMPGKVLQSLNKVGTRNPLFLLDEIDKLGMDFRGDPSSALLEVLDP
                     EQNHTFSDHYVEVDFDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKTNIAIK
                     YLLPKQMKNNGVKDEELSVSEDAVRDIVRYYTREAGVRSLERELSKICRKVVKALQLG
                     EMKPKVVVTPENLNDFLGVRKFSFGRAEQQNQVGQVVGLAWTEVGGDLLTIEAATMPG
                     KGNVQRTGQLGDVMKESVEAARTVVRSRARVLGIKDEVFEKKDIHIHVPDGATPKDGP
                     SAGIAMTTAIVSALTGIPVRGEVAMTGEITLRGEVTAIGGLKEKLLAALRGGIKTVII
                     PEENVKDLQEIPANVKQGLKIIPVKWIDKVLEVALERQPVALTDEEAEPAQVGSTAPL
                     AKPKSGRGAAVKH"
     gene            16074..16149
                     /locus_tag="PK242_03415"
     tRNA            16074..16149
                     /locus_tag="PK242_03415"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16107..16109,aa:Gly,seq:gcc)
     gene            16376..18319
                     /locus_tag="PK242_03420"
     CDS             16376..18319
                     /locus_tag="PK242_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOZ93098.1"
                     /translation="MALSDLTVRQARTTGKRYTLSDNDCLSLMVSAAGGKSWIFRYYW
                     LGKQKRLSLGGYPALSLREARAERDKAQALLARGVDPQIERDQRRHAAKLAGEYTFKN
                     VFDAWVEHRRKELKEGRQSTLSQILRIFNKDVLPTLGKMSIYDIRRPQLVGVVAAIEK
                     RKAFTTAEKVRTWFNQLFRYALVIAEGLEVNPAADLDVVAEPKPPVAHNPYLHLPEMP
                     EFLQKLRLYNPRGWQTQLGVRLLFLTGVRTGELRLAEPEQFDLDRGLWIIPPQVVKQL
                     QDEMRKAGKRPQDVPPYIVPLSLQAIEIVRYLLGVMRPAQKYLLSHRSELKKRISENT
                     LNKAVQLMGYEGRLTGHGIRGTISTALNEIGYPKIWVDAQLSHSDPNKVSSAYNHAKY
                     VEPRRRMMQDWADRLDLLEQGEVEAASVHLTIRIDGVPAMAEVEEAMSAVPTVAKPAV
                     PGVPPVVATPIVVTPNSGGITFQRLSQVPQPPVHVPEPEVSAIQREREEMLAIYESPN
                     NLPVPLFGKLAGKSKDQINRELKAGKLLSISLGNRGQRVPDWQLVPLKHKLAQVLMNQ
                     CPHANSWKLYHMLTQPHPDLGDRAAIDAVTPTNVPAIIRVIMGDYQRHEDGPEAVALR
                     PIPEDVRLSVRRLVDSAAVLEGA"
     gene            complement(18466..19500)
                     /gene="mobH"
                     /locus_tag="PK242_03425"
                     /pseudo
     CDS             complement(18466..19500)
                     /gene="mobH"
                     /locus_tag="PK242_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
     gene            19812..20438
                     /locus_tag="PK242_03430"
     CDS             19812..20438
                     /locus_tag="PK242_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HOZ93099.1"
                     /translation="MPTAIEFIADRLPRVTVEDVRRFADTVEIRDAPAFAAELQAFIH
                     ERVEAVTLPANLEGETVAQALERKAAALRADTRWAPTETDVQRGRAALLAAFNQPDNL
                     PPAEFAKLADKSRQQIYKDILARRLLALNVGPRGQKLPDWQLDPVKQQLTQTVLQEVE
                     GIDHWTIYRALSEPLEGLGGRSPVDAVTHGTIDDVAEAVFNVLGVQVH"
     gene            20451..21083
                     /locus_tag="PK242_03435"
     CDS             20451..21083
                     /locus_tag="PK242_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HOZ93100.1"
                     /translation="MSHELPSFLIDAGELLQHVSRVVYRGSPLYYGRSSTNRYDDPAQ
                     AYGVLYLGRDLPTALMESVFHKHQWLADTKRSIALKEVQSRMVRAVGVMDDVLLADLT
                     APGVMAGYFGLNLEQLASRDYTHTQRVSAQVHAMLGDDGQALFDGVLYPSRNNYPAKS
                     IALFERAGAKVGVIEDIDMVDHVDWPRFVATYRIGVEPDPGPVEPEDEAS"
     gene            21168..>21373
                     /locus_tag="PK242_03440"
     CDS             21168..>21373
                     /locus_tag="PK242_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3742 family protein"
                     /protein_id="HOZ93101.1"
                     /translation="MNTTTRTSTAERLGRAFGRGWRAYARGERRASNWLVSKGMPRAG
                     ATALVWVAATIGNLREASIAGVVVG"
BASE COUNT         3657 a         7127 c         7385 g         3204 t
ORIGIN      
        1 cgttcgggca ccgtccgcag cacatggaaa ccgacattgg agcgggcgat gggaatcgaa
       61 cccacgtctt gagcttggga agctcaggtc ctgccattga acgacgcccg cggtgaagag
      121 cgccgattct agcggagcgc gcgccgcctg actttcaatc ctgcaggatc tggccgtcgt
      181 gctcgacgta gggcgggtac tggtggccgg tgctgctgct ttcggcctgc gaggcggcga
      241 tgaaggcgcc gcgctgcacg tcgaacacgt gcacctcgcc atcctctatc acgtagtgcc
      301 agccgtgcag cgcgatgcgg ccatcctgca cgcgccggcg caccatcggg tagtccatca
      361 ggcgctcgag ttgcagcacc accgcgcgct gctcggtgcg gcgcagcgcc tcgtccgaga
      421 cccgcaccgg cagcgtcgcg gggcggccca ggtcgagcca gcgctgcagg ttgtgcgcct
      481 cggccggcac ctcgccgtac agcgccttga tggcgccgca atggctgtgg ccgcagacga
      541 tgatgcggct gacttcgagg ctgagcacgg cgaactcgat cgccgccgcg gtgccgtggt
      601 ggccgtgcga gccatcgtgc ggcggcacga aggcgccgac gttgcgcacc atgaacggct
      661 ggtggcttcg gcggccagcg cgctggccgt gtacagcgcg gtcaggcgct ggttgtgttc
      721 ctctcgcgca ccttgcgctc caggttctgg tgcgaagcct gcagcgtgcg cgtcatcagg
      781 ttgaagccgt cggccagctg gccgaattcg tcgtcggtgt cgacctcgag tcgggtgccg
      841 aggtcgccct ggcgcacccg tgccagcgcc tgctgcagcc gcgtcaccgg gttgaggacc
      901 agcaaatggc tgagcgccat gaaagccacc acggcggcga tcgccagcgc catcatgaac
      961 agctggaaca ggtgcagcgt ggcggtccag cgcatgatcc gccattcgat cgcctgcacg
     1021 aaatcgtcga tttcgctgac gaaggcgtcg ccgcgcgcca ccgcggcggc gccgtcgtcg
     1081 gccggcaggt tgagcgccga ttcggcctgc agcgccagcc actgggcgcg gatgtgcccg
     1141 tactgggcgc gcgtttcgtc gttccatggc accgccaggg ggcgcacggg gtcgcccgag
     1201 cgcagcagct ccaggccatc gtcgaagcgc tggcgcaggc gcgccagttc ggccggcgat
     1261 tcattctgct gtgccagcag gatgcgcagc atgttcatgc gcaggcggcc ggcttcgttg
     1321 acggcggcgg cgccgccttc cagctgccag gtgacccaca gcgtgaagct gatcgacacc
     1381 agcgccacga gcagcaggga catgcccatg gcgagcacct tggtcgacag cggcggcttc
     1441 ttgcgcttgc agcagtttaa ggacgaacgg cgcgcggcgc agccgtcagc ctatcgcctg
     1501 cgcggcgtgc aggccgctgc gcaccgcgcc ttccagcgtg gccgggtacg ggccttcgat
     1561 gtagtcgccg gcggccagca ggccgggcgc gatgcgcgcc agcggccgct gcagacccgg
     1621 cgtgcaggcg aaggtggcgc ggcgctcggt cacgcacagc agcggcgtca gcgccaggcc
     1681 gagctgcgcc tgcgcctgcg ccagcagcgc agcctgcagc gcgtcgcgct cgccggcgct
     1741 gttgctgacg acgaaggcca gcaggccggt gggcgcgtcg gccggcggcg cgatggcgtc
     1801 gcgctcgaac acgaactgcg ccggcgccga cgggccgctg gccagcgcca gcatcggcgc
     1861 gcgcagcagc cgcgcgccag cagcgggcgg cgccgcctgg gcgtacacgg tggtgatcgc
     1921 tcggtgcgcc aggccctggg tgccggcagc ccacgcgcga agcgagtctg ctattgaatc
     1981 aggagctgat ggcgccgttg ccatcccgac ctgcgccgcg tggggcgccg aagtggcgag
     2041 gatcacctgg tcgaacgcct ggccgtcgat ggcccagccg ccgggttgcc actgcagcgc
     2101 cgccacgcgc tgaccggtca gcacgcgggc gccgcgcgcc tgcagccagc gcgccgcggc
     2161 ctgcggcagc agcgcgccca gatcgacccg cggcaacaac aggttcgagc cgccgcgcac
     2221 gccgaacagc gcgtcgcgca gcacgcgcag gaacacctgc gcgctggcct cggccatcgg
     2281 tgtgttgagc gccgatacgc acagcggctc gatgaactcg gcgcgcaggc gcggcggcag
     2341 cgcgcggcac agatcggcca cgctgagctg cggcgggcac acaaagccgg cgcgccgcca
     2401 gccgagcgcg gccgccagca gcgcccgctt gtcgcgccac gaccagccgc gcgcagccag
     2461 gatgccggcc gcggcgtcga gcggcgccgg ccagtcgggc agggccaggc cgctgccgtc
     2521 ggggtacatc agcgtcagcg gccggcgcag cagcgcctgc ccggggtcga cgccgaggct
     2581 gcgcatcagc cgcaggcagt cgatataggc gccgatcagg atgtgctggc cgttgtcgac
     2641 catgagctcg cgcccgtccg gcagcgtcag cggcaggccg cgcgcgcgcc cgccgagctg
     2701 gcgcgcagct tcgaacaccg tcacgtcatg ccccagctcg accgcgcgca ccgccgccgc
     2761 catgccggcc cagccggcgc cgacgatggc gacgcacagg gcgcttccgt cagcccgctt
     2821 catgggtgat atcggctcgt agcccgcgcc gataaagcgc aaacagctgc cgaaaaaata
     2881 gcaagcaagg tttgagcctg cgcggcgtcg taccgctggc gcggtgtcca cgcaggggtc
     2941 gtgaagccgc gccgcgcgcc atgccgggga aggccgtcgt gcgcccggcg cctgctggcg
     3001 ccacggcggg ccccggccgc ggcgaggcga gtgccggcct caaaaccgcc ccagcgcctg
     3061 catcttccag gccagccaca gcttgcgcag cggggtcagg gcgatgcgct ggcgcagcac
     3121 ctgaaagccg tccgcctcga tctcgcgcag cagcgtgcgg tagatgctgg ccatcatcag
     3181 gcccggcttc tggctgcgcc ggtcggcggc cggcagcagc gccagcgcct cgtcgtacag
     3241 gcggtgcgcg cgctcggcct gaaagcgcat cagccgctcg aagcggtccg aatgctggcc
     3301 gttcaggatt tcgtgcgcct tgacctcgaa gcgctgcaac tcgtccaccg gcaggtagat
     3361 gcggccgcgc cgcgcgtctt cgccgacgtc gcgcaggatg ttggtgagct gaaacgccag
     3421 ccccagccgg tgcgcgtagg ccgtggtgcg ctggtcctgc tggccgaaga tgcgcgccga
     3481 gacttcgccc accacgccgg ccaccaggtg gcagtagcgc tgcagctgcg cgaagtcgag
     3541 gtagcgcgtc tgcgtcaggt ccatctggca gccttcgatc acggccagca gctggcgcga
     3601 ctcgatgccg aaggcggccg cgtgcggcat cagcgcctgc agcgccgggt gcgtcggctg
     3661 gccgccgaag gcgcgcccga cttccgactg ccaccaggcc agcttggtgg ccgccacgcc
     3721 agggtcgctg acctcgtcga ccacgtcgtc cacctcgcgg cagaaggcat agaacgccgt
     3781 gatggcggcg cggcgcggcg gcggcagaaa caggaaggcg taataaaagc tgctgcccga
     3841 agcggcggcc ttctgctgca cgtagtcttg cggggattgc ggtggcgcgg tcacgcgggc
     3901 gattgtccca tcgggtacgg tcgggccggc cgagcggcct gagcgcgcgg cgcgccggcc
     3961 gtgcgggggc tcacatccac agcatgcgcc aggcaatcag cggcgcgtcg gctttgccga
     4021 cggtgggacg gcgtgccagc gtgtcgaagc cctgcgcggc gatcttgtcg aggatgcgca
     4081 ggccgccctg caccacggcg cgcagctccc agccggcgcg gcccggcacg cgatgcacca
     4141 gattggcgcc tttttgcatg gtggcccgcg cgtggtttgc gcaggtagct atcaagttga
     4201 tagtgttcgg gtcgctgcgc agggccgcga aatcgcgcgc cggctggcgg atgcgcacgc
     4261 cgtgcgcggc gcagtcggcc agcggcaggt agtagcgtcc gcgcggaatg tccacgctca
     4321 ggtcctgcca gaaattgatg agttgcagcg cgctgcagat ggcgtcgctc tcggccagcg
     4381 cgcgctcgtc gcgcacgcca tacaggtgca gcagcagccg cccgaccggg ttggccgagc
     4441 ggcggcagta gtcgagcaac tcggccatgt cggcgtaggc ggcgccgtcg cgcgtgaagg
     4501 ccacgtcctg cgcgaaagcc gacagcaggt cgtccagcgg ctgcagcggc agcgcgtggg
     4561 cacgcatctg cgccgccagc ggcccgaaca cctgcggcca gcgcgcggac gggctgcggc
     4621 cagccgccac cgcgtgcaga tcggcccggt acgccgccag gtcggccagg cggcgcgccg
     4681 gcgcggcttc gccctcgtcg gcgatgtcgt cggcggtgcg cgcgaagtgg tagatggcgc
     4741 cgatcggcgg gcgcaggcgc ggcgggcaaa gccacgacgc gaccgggaaa ttctcgtagt
     4801 gcgccaccgc ggcgggcagc gcgctggcgg cgtcggcggg cggcgaaggg cgggcggaca
     4861 tgggccgggc gattgtgccc gccaggcgcg gccgccggcc ggcgtccggc tttacactcg
     4921 cgggccggac cattagatta ctaaccggtc agtcattaac gtgagcatgc ttactaaccg
     4981 cccactggcg ccggctggcg ccccggccgc cgcgaccgtg tcgcgccggc gctcgaccgg
     5041 cgccgtcggc gccgcgctgc tggccggcct gtgcgccacg ctgctggccg gctgcggcaa
     5101 ggcgccgcca gcggccgaac ctgtgcgcgc cgtcaagctg atcacggtgg cgccggcggg
     5161 gctgcagacg gcgcccgaat acgtcggcga ggtgcgcgcg cgggtcgaat ctcggctcgg
     5221 ctttcgcgtc gccggcaaga tcacccggcg cgcggtcgat gtcggccagc gtgtggcggc
     5281 aggccagctg ctggcgcagc tcgatgcgcg cgactaccag ctggcggccg acgcggcccg
     5341 cgcgcaactg agcgccgcca ccacgcagcg cgacctggcc gcggccgacc tcaagcgctt
     5401 tcaggggctg cgcgagcagg gcttcgtcag cgccgccgaa atagagcgcc gcagcgccca
     5461 gctgcaggcc gcgcaggcga cgctggagca ggcgcgcgcc cagctgaacg cgcagggcaa
     5521 ccagaccggc tacacccagc tgctggccga cgcgccgggc gtggtcaccg ccgtcgaggc
     5581 cgaggtcggc caggtggtgg cggccggcac gccggtggtg cgcatcgcgc aagacggcgc
     5641 tcgcgacgcc gtgttcgccg tgcccgaaga ccgcgtggcc gcagtgcgcg tcggccagcc
     5701 ggtcggcgtg cgcgcctggg ccggcggcag ccaatggccc ggcacggtgc gcgaagtcgc
     5761 cgccagcgcc gacccggtga cccgcacgtt tgccgtcaag gtggcgctgg ccggcgaccc
     5821 gcctccgctg ggagctactg tttacgtagc cccggcggcg gacgagcgtg tcgccgccgg
     5881 cgtcatcaag ctgcccacca gtgcgctgcg ccacgacggc aacggcagcg cggtgtggct
     5941 gtacgacgcc gccagcggca gcgtgcgttt gcagccggtg cagctggccg gcccggacgg
     6001 caacgaggtc gtcgtggcca gcggcctgac cccgggcatg caggtggtgg ccaccggcgt
     6061 gcatgtgctg acgcccggtc agaaggtgag cgtttatgtc ccgaatgtgg cgccggccgg
     6121 cgccgaagtt ggctctcacg ctatgaaacc aatagctaat ggcgccggtg gcggggccgc
     6181 ggcgagcgcc gcgccggctg ccagccgctg accgacgggc cgccgccatg ccgcaccaga
     6241 gcccgatcga cggcgctgcc gacgccgccg ccacgcctgc caagccgttc aacatctcgc
     6301 gctgggcgct ggaacactcg gcgctcacgc gctacctgat ggtggtgctg atgctgctcg
     6361 gcgtggcggc ctacttccag ctgggccagg acgaagaccc gccgttcacc tttcgcgtga
     6421 tggtggtgcg cgccttctgg ccgggtgcca cggcgcgcca ggtggccgac caggtggccg
     6481 acaagatcga gcgcacgctg caggaagtgc cttacgccga caagatccgc agctacagca
     6541 agcccggcga ggcgctgatc atcttccagc tcaaggacag ctcgccgccg cgcgacgtgc
     6601 cgcagctctg gtacacggtg cgcaaaaagg tcggcgacat gcgccagacg ctgcccgcgg
     6661 gcgtgcaggg gccgttcttc aacgacgact tcggcgacgt ctacggcgtc atctacgcgc
     6721 tgtcgggcga gggcttttcg ccggccgagc tgaagacctt cgccgacgat gcgcgccagc
     6781 gcctgctgcg cgtgaaggac gtcgccaagg tcgagctgtt cggcgtgcag gacgagaagg
     6841 tccacgtcga gatctcgcag aagcgcctgg cccagctcgg cctggacatg ggccaggtgc
     6901 tgggccagct cgggtcgcag aacgcgatcg agtcggccgg cgccgtgcag acgccgctcg
     6961 acgtggtgca ggtgcgcgtg gcgggccagt tcaacgacct cgagcagctg cgcgcgatgc
     7021 cgatccgcgc ccccaacggt cgccagctgc gccttggcga catcgccagc gtcacccgcg
     7081 gctacgtcga tccgccgcag gtcaaggtgc gccacgacgg ccgcgaagtg atcgcgctcg
     7141 gcgtgtcgat ggccaagggc ggcgacatca tcgcgctcgg caagggcctg cgcgaagcct
     7201 cggcacagat cgaacgcacc ctgccgctgg gcgtgtcgct ggcgcagatc caggaccagc
     7261 cgggcgcggt ggccagttcg atcaacgagt tcgtcaaggt gttgatcgag gcggtggtga
     7321 tcgtgctggc ggtgagcttt ctgtcgctcg ggctgcacaa gcgccctggt cagcacccgc
     7381 tgtggcgccg ctggaccctg gacgtgcgcc ccgggctggt ggtgggcatc accattccac
     7441 tggtgctggc ggtgaccttc ctggccatgc actactgggg catcgggctg cacaagatct
     7501 cgctcggctc gctgatcatt gccctgggcc tgctggtgga cgacgccatc atcgcggtcg
     7561 agatgatggt gcgcaagctg gaggagggct acgacaagct gcgcgccgcc accttcgcct
     7621 acgagatcac cgccatcccg atgctgaccg gcacgctgat caccgccgtg ggcttcctgc
     7681 cgatcggcat ggccaagtcg accaccggcg aatacacctt cgccatcttc gccgtcacgg
     7741 tggtggcgct gctggtcagc tgggtggcct cggtggtgtt cgtgccctac ctgggcgcct
     7801 ggctgctgcg cctgccgccg cacgtgcagg cggcgcgcgc agaggcgctc aaccgcggcg
     7861 tgccggtgac cgaagagccg cacgagctgt tcgacacgcc gttctacaac cgctttcgcg
     7921 ccgcggtgag ctggtgcgtg gcgcaccggt ggttgaccat tggcgccacg gtcttggtgt
     7981 tcggcctcgg cctgttcggc atgacgcgcg tgcagcagca gttcttcccg gattcggtgc
     8041 ggccggaaat cctggtcgac ctgtgggcgc ctgaaggcac cgccttcgtc gccaccgagg
     8101 aggtcgcgcg ccgcgtcgag gcgcgcctgc gcggcgagcc gggcgtgcgc ggcgtcaccg
     8161 cctgggtcgg ctcaggggtg ccgcgttttt atctgccgct cgaccagatc tttccgcaga
     8221 gcaacgtggc gcagcttatc gtggtggcgc aggacctggc tgagcgcaac cggctgcggc
     8281 tcgacctggc ggcgctgctg gcgcgcgaat ttcccgaggt gcgcgcgcgc gtgaaattcc
     8341 tgcccaacgg gccgccggtg gcctacccgg tgcagttccg cgtgctcggg cccgacccgg
     8401 ccacgctgcg tgcgcgcgcc gacgaggtgc gggccgcgct gcaagcgcac cccaacacgc
     8461 gcggcgtgaa cgacaactgg aacgaatcgg tcaaggtggt gcggctggag atcgaccagg
     8521 ccaaggcgcg cgcgctcggc gtcagcagcc agtcgatcgc gcaggccggg cgcacgctgc
     8581 tggccggcag caccatcggg cagttccgcg agggcgacaa gctgatcgac attgtgctgc
     8641 gccagccgct ggccgagcgc gatgccatca ccgacctggc atacacctac ctgccgacgg
     8701 cgagcggcca gtcgattccg ctcacgcaga tcgcgcgccc ggtgttcgac tgggagcccg
     8761 gcgtgctgtg gcgcgagaac cgcgactacg cgatcaccgt caacgccgac gtcgccgagg
     8821 gactgcaggg cgccaccgtg accttcgcgc tgcagcccga gctgcaggcg atgcaggcgc
     8881 gctggcatgc ggctggccag tcggcgtacc gcatcgaagt ggccggcgcg gtggaggaaa
     8941 gcagcaaggg gtcgtcgtcg atcgcggccg gcattccggc catgctgttc atcaccttca
     9001 cgctgctgat gctgcagctg cactcgttca gccgcgcgct gctggtgttc atcaccgggc
     9061 cgctgggcat cgcgggcgtg gcggcggcgc tgctgctgct gaaccggccg ttcggcttcg
     9121 tggcgttgct cggggtgatc gcgctgatgg ggatgattca gcgcaactcg gtgatcctga
     9181 tcgaccagat cgagaaggac cgcgagcgcg gcgtgccggc ctgggacgcc atcgtcgagg
     9241 cggcggtgcg ccggctgcgg ccgatcgtgc tgacggcggc ggcggccgtg ctggccatgg
     9301 tgccgctgtc gcgcagcgtg ttctggggcc cgatggcggt cgccatcatg ggcgggctga
     9361 tcgtggccac cgcgctcacg ctgctggcgc tgccggccat gtacgcggcc tggtttcgcg
     9421 tcaggccgcc ggcgcgggaa aacccggcgc cagcgcccgt gagcgggcgt tcgtcggcaa
     9481 gctaaaatag ccggttcgcc agaatccgac gggcggctcg ccacgcgcga tccgcccgcg
     9541 tcatttgaaa cgccgtgcgc gggtggcgaa attggtagac gcaccaggtt taggtcctga
     9601 cgccagcaat ggtgtggggg ttcgagtccc cccccgcgca ccacccatca caacttagga
     9661 agcgagaacc ccatggccgt gaacgtggaa acccttgaaa agctcgggcg caagatgacg
     9721 ctcacgctgc ctgtcgagac catccagagc gaggtcgacg cccgcctgcg caagctggcc
     9781 cgcaccgtca agatggacgg ctttcgcccc ggcaaggtgc cgctgagcgt ggtggcacgc
     9841 cagtacggct attcggtgca gtacgaggtg atgaacgaca aggtcggtga ggccttcttc
     9901 aacgccgcca acgaagccaa tctgcgggtg gccggccagc cgacgatcac cgagaaggac
     9961 ggcgcgcccg aggggcagat ggcgttcgac gccgtgttcg aggtgtttcc cgatgtcaag
    10021 atcggcgacc tgtccgtcgc cgagatcgag aagctgtcgg ccgacgtcgg cgacgaggcg
    10081 atcgaccgca cgctcgagat cctgcgcaag cagcgccgca gcttcgccca gcgcgccaag
    10141 gatgcgccgg ccgaggacgg cgaccgcgtc acggtcgact tcgccggcaa gatcgacggc
    10201 gagccgttcg aaggcggcaa ggccgaggac ttccagttcg tcatcgccga ggggcagatg
    10261 ctcaaggagt tcgaggatgc ggtgcgcggc atgaaggtcg gcgaaagcaa gaccttcccg
    10321 ctggccttcc cggccgacta tcacggcaag gacgtggccg gcaagacggc cgacttcctg
    10381 gtcaccgtga acaagatcga agccgcccac ctgcccgaag tgaacgagca gctggccaag
    10441 tcgctcggcg tgaaggacgg caccgtcgaa ggcctgcgcg ccgacatccg caagaacctc
    10501 gagcgcgagg tgaagttccg cctcaaccag cgcaacaagc aggcggtgat ggacgcgctg
    10561 gtcgccaagg ccgagctcga catccccaat tccagcgtgc aggccgaact cgaccgcatg
    10621 gtgcagtcgg cccgcgccga cctgaaggcg cgcggcatca aggacgtcga gaagctgccg
    10681 atccccgagg acggcttccg tccgcaggcc gagcagcgcg tgcgcctggg cctggtgctg
    10741 gccgaactgg tcaaggccaa cgacctgcag gccaagccgg agcagatccg cgcccaggtc
    10801 gaagaactgg ccgccagcta cgagaagccg gaagaggtgg tgcgctacta ctacgccgac
    10861 aaccgccggc tggccgacgt cgaggcgatg gtgatcgaga acaacgtgac cgagttcgtg
    10921 ctcggccgcg ccaaggtcac cgagaagaag ctcggcttcg acgagctgat gggtcagcag
    10981 ggctgattta ctcagctttt aatagctgct ggcgcaaatc tggcgccggc ccgcggccga
    11041 tgacgcactg aactggccgg cgcagcggcg gcaccggcgc cgcagcgaca atggggcttg
    11101 gaatcgcacg gttccggccc catttgcatg cacagcacac cccttggagc tttcatgagc
    11161 caatacgaca cccccagcaa cctcggcctc atcccgatgg tcatcgagca gtccggccgc
    11221 ggcgagcgct cgtacgacat ctattcgcgc ctgctgcgcg agcgcgtggt gttcctggtg
    11281 ggcgaagtca acgaccagac cgccaatctg gtggtggcgc agctgctgtt cctggaaagc
    11341 gagaatcccg acaaggacat ctcgctgtac atcaactctc ccggcggcag cgtcacggcc
    11401 ggcatgtcga tctacgacac catgcagttc atcaagcccg atgtctcgac catgtgcctg
    11461 ggcttcgcgg ccagcatggg cgccttcctg ctggcagccg gcgccaagaa caagcgctac
    11521 agcctgccca acagcaaggt catgatccac caggtgctgg gcggggcgcg cggccaggcc
    11581 accgacatcg agatccatgc gcgcgacatc ctgcgcacca aggaccagat gaaccgcatc
    11641 ctggccgagc gcaccggcca gccgctggaa aaaatccagc gcgacaccga acgcgactac
    11701 ttcatgaccg ccgacgaggc caaggactac gggatcgtcg atcaggtcat cgccaaccga
    11761 ccctgagcgc ccccggcggc gcgagactct gagcgccccc cgcggcgcga gactctgagc
    11821 gcgcccggcg gcgcgcgact gcggccggcc tttcccccct tcggtgcatg cccctggcgg
    11881 cagcgtgctg gcgcacgcgg ccgcgagtct gatttggtta tcatgcgccg atcccccacg
    11941 agactgacac ctgatggccg acaagaaagg ctcttcttcc agcgaaaaga ccttgtactg
    12001 ctcgttctgc ggcaagagcc agcacgaggt caagaagctg atcgccggcc cctcggtatt
    12061 catctgcgac gagtgcatcg acctgtgcaa cgacatcatc cgcgaagagg tgcccgaggg
    12121 cgccgccgag ggtgaagcca ggagcgacct gccgacgccg gccgacatca aggccaacct
    12181 cgacaactac gtgatcggcc aggaaaaggc caagcgcacg ctgtcggtcg cggtctacaa
    12241 ccattacaag cgtctcaagc acaaggaaga cgccaagggg ggtgacgtcg aactggccaa
    12301 gagcaacatc ctgctgatcg ggccgaccgg ctcgggcaag acgctgctgg cgcagacgct
    12361 ggcgcgccag ctcgacgtgc ccttcgtgat ggccgacgcg accacgctga ccgaagccgg
    12421 ctacgtcggc gaggacgtcg agaacatcat cagcaagctg ctgcaaagct gcaactacga
    12481 cgtcgagcgc gcccagcgcg gcatcgtcta catcgacgag atcgacaaga tcagccgcaa
    12541 ggccgacaac cccagcatca cgcgcgacgt ctcgggcgag ggcgtgcagc aggcgttgct
    12601 taagctgatc gaaggcacca tggccagcat cccgccccag ggcgggcgca agcatccgaa
    12661 ccaggatttc ctgcagatcg acaccaccaa catcctgttc atctgcggcg gcgcgttcgc
    12721 cgggctcgaa aaggtcatcg aggcacgcac cgaggcctcc ggcatcggct tcggcgcgac
    12781 cgtgcgcagc aaggcgcagc gctcgatcac cgaggtgttt caggaggtcg agccggaaga
    12841 cctgatcaag tacggcatca tccccgagct ggtgggccgc atgccggtgg tggcgacgct
    12901 ggccgaactg accgaggacg cgctggtgca gatcctgacc gagccgaaga acgcgctggt
    12961 caagcagttc ggccgcttgc tgcagatgga aggcgtcgag ctcgagatcc gccccagcgc
    13021 gttgaaggcg atcgcgcgca aggcgatcgc ccgcaagacc ggggcgcgcg ggctgcgctc
    13081 gatcatggag caggcgctga tcgacaccat gttcgagttg ccccacaccg ccaacgtggc
    13141 caaggtggtg gtcgacgagt ccaccatcga ggacgcccag ccgccgctgc tggtgtaccg
    13201 cgaagcggct aagaaggcct gaaggcgcct gcgcggcgtc ggccgtcgct tggcgcgacc
    13261 gccgcagggc acggcttggc cgctggccag gcggctgagg ctgtaaatgg ctgaatgccc
    13321 gcgcccatat tgcctatgca gctacttatt tgatagctaa cggcggcgcc gcccgcgcgg
    13381 gtgcgctggg tttaacgggc cttttcacgc ggcctgcgcc gctgggttga aaatggcgtc
    13441 tgagcagcca tctctggctg aatcacccga aggattctcg atgtccggaa ccaccccgtt
    13501 gcccgccacc ccgatcgacc tgccgctgct gccgctgcgc gacgtggtgg tgtttcctca
    13561 catggtcatc ccgctgttcg tcggtcggcc caagagcatc aaggcgctgg agaccgcgat
    13621 ggcggccgac cgtcgcatca tgctggtggc acagaagacg gcggccaagg acgagccgtc
    13681 ggtcaccgac atgttcgaag tcggctgcgt ctcgacgatc ctgcagatgc tcaagctccc
    13741 ggacggcacg gtgaaggtgc tggtcgaagg ccagcagcgc gccaccgtca cgcacatcga
    13801 ggacggcgag gcgcatttcg tcgccaccgt gacgccgctc gacggtgaag ccgcggacaa
    13861 ggacaacgcc gaggtcgagg cgttgcgccg tgccgtgatg cagcagttcg accagtacgt
    13921 taagctgaac aagaagattc cgcccgagat cctgacctcg atcgccagca tcgacgagcc
    13981 gggccggctg gccgacacca ttgccgcgca cctgccgctc aagctcgaga acaagcagct
    14041 ggtgctcgac ctggccagtg tgaaggaccg tctggacaac ctgttcgagc agctcgagcg
    14101 cgaagtcgac atcctcaacg tcgacaagaa gatccgcggc cgcgtcaagc gccagatgga
    14161 aaagaaccag cgtgacttct acctcaacga gcaggtcaag gcgatccaga aggagctcgg
    14221 cgaaggagaa gagggcgcgg acatcgagga aatcgagaag aagatcaagg ccgcgcgcat
    14281 gcccaaggag gcgcgcaaga aggccgacgc cgagctgaag aagctcaagc tgatgtcgcc
    14341 gatgtcggcc gaggcgaccg tggtgcgcaa ctacatcgac gtgctggtcg gtctgccgtg
    14401 ggccaagaag accaagatca agcacgacct gggctacgcc gagcaggtgc tcaatgaaga
    14461 ccactacggc ctggaaaaag tcaaggaccg catcctcgag tacctggccg tgcagcaacg
    14521 ggtggacaag gtcaaggccc ccatcttgtg cctggtcggc ccacccggcg tgggcaagac
    14581 ttcgctcggt caatcggtgg ccaaggccac cgggcgcaag tacgtgcgca tggcactggg
    14641 cggtatgcgc gacgaggccg aaatccgcgg ccaccggcgc acctacatcg gcgccatgcc
    14701 cggcaaggtg ctgcagagcc tgaacaaggt cggcacgcgc aacccgctgt tcctgctcga
    14761 cgagatcgac aagctgggca tggacttccg cggcgacccg tcgagcgcgc tgctcgaggt
    14821 gctggacccg gagcagaacc ataccttcag cgaccactac gtcgaggtcg atttcgacct
    14881 gtcggacgtc atgttcgtgg cgacttcgaa ctcgatgaac attccgccgg cgctgctgga
    14941 ccggatggag gtcatccgcc tgtcgggcta taccgaggac gagaagacca acatcgccat
    15001 caagtacctg ctgcccaagc agatgaagaa caacggcgtg aaggacgagg agctgtcggt
    15061 gtctgaagac gccgtgcgcg acatcgtgcg ctattacacg cgcgaagcgg gcgtgcgctc
    15121 gctcgagcgc gagctgtcga agatctgccg caaggtggtc aaagcgctgc agctgggcga
    15181 gatgaagccg aaggtggtgg tcacgccgga aaacctgaac gacttcctgg gcgtgcgcaa
    15241 gttcagcttc ggccgtgccg agcagcagaa ccaggtcggc caggtggttg gcctggcctg
    15301 gaccgaggtc ggcggcgacc tgctcaccat cgaggccgcc accatgccgg gcaagggcaa
    15361 cgtgcagcgc accggtcagc tcggcgacgt catgaaagaa tcggtcgagg ccgcacgcac
    15421 cgtggtgcgc tcgcgggcgc gcgtgctggg catcaaggac gaggtgttcg agaagaagga
    15481 catccacatc catgtgcccg atggcgccac gcccaaggac gggccgagcg ccggcatcgc
    15541 catgaccacc gcgatcgtct ccgccctcac cggcattccg gtgcgcggcg aagtggcgat
    15601 gaccggcgag atcaccctgc gcggcgaagt cacggccatc ggcggcctga aggaaaagct
    15661 gctggccgcg ctgcgtgggg gcatcaagac ggtgatcatc ccggaagaga acgtcaagga
    15721 cctgcaggag atcccggcca acgtgaagca aggtctgaag atcatccccg tcaaatggat
    15781 cgacaaggtg ctggaggtgg cgctggaacg ccagccggtg gcgctcacgg acgaagaggc
    15841 ggaaccggcg caggtcggca gcaccgcgcc gctggccaaa cccaagtcgg gccgcggcgc
    15901 ggcggtgaag cactgagccc gcaagcggca atgggccgct cgaacccttg gggcgggcgc
    15961 atccctgcag ggtttggcgg cttcgaggct tcgctggcct gcacctgcat aaaaaaattt
    16021 cgagccgaag gtgacaagtc ctgaaattca ggctatactg caaggcttgc aacgcgggaa
    16081 tagctcagtt ggtagagcgc aaccttgcca aggttgaggt cgcgagttcg agcctcgttt
    16141 cccgctccaa gttatgatct acagacttcc actgacgtct gtaagtcgtt gaaaagaggg
    16201 gcttcggccc cttttttcat tccagcgagc gccacggaag tccacccaca gctaccgttt
    16261 ttgagtgacc aattgaggca caagaatacg gttgggacgg ctaccggata gttgctgggt
    16321 ctgagcggga accatgacga gcattaggcc taagtcctaa cttaggagcc tcgaaatggc
    16381 actctctgat ctgaccgtcc ggcaggcaag gaccaccggc aagcgctaca ccctctccga
    16441 caacgactgc ctgagcctga tggtctctgc cgcaggcggc aagtcgtgga tcttccgcta
    16501 ctactggctg ggcaagcaga agcgcttgtc cctcggcggc tatcccgccc tcagcttgcg
    16561 cgaggcccgc gccgagcggg acaaggccca ggccctgctc gccagggggg tcgatcccca
    16621 aatcgaacgc gatcagcgtc ggcacgcagc caagctggcg ggcgaataca ccttcaagaa
    16681 cgtcttcgat gcctgggtcg agcatcgccg caaggaactc aaggaaggcc gtcaaagcac
    16741 gctctcgcag atcctgcgca tcttcaacaa ggacgtgctg ccgaccctgg ggaagatgtc
    16801 gatttacgac attcgtcggc cccagctcgt gggcgtcgtg gcggcgatcg agaaacgcaa
    16861 ggcgttcact accgccgaga aggtccgcac ctggttcaat caactgttcc gctatgccct
    16921 ggtcatcgcc gaagggctgg aagtcaaccc ggctgccgat ctggacgttg tggccgagcc
    16981 caagcctccc gtggcccaca acccctacct gcacctgccc gagatgccgg agttcctcca
    17041 gaagctccgg ctctacaacc ctcgtggctg gcagacccag cttggcgtcc gcctgctgtt
    17101 cctgacgggt gtgcgcacgg gcgagctgcg tttggcggaa cctgaacagt tcgacctcga
    17161 ccgtggcttg tggatcatcc cgccgcaggt ggtcaagcag ctccaggacg aaatgcgcaa
    17221 ggccggcaag cggccgcagg acgtgccgcc ctacatcgtg cccctgtccc tgcaggccat
    17281 cgagatcgtg cgctatctcc tgggcgtgat gcgtccggcg cagaagtacc tgctgtcgca
    17341 tcgcagcgaa ctcaagaagc gcatcagcga gaacaccctc aacaaggccg tgcagctcat
    17401 ggggtatgag gggcgtctga ccggccacgg catccgcggc accatctcga ccgcgctcaa
    17461 cgagatcggt tacccgaaga tttgggtgga cgcgcagctt tcgcactccg accctaacaa
    17521 ggtcagctcg gcctacaacc acgccaagta cgtggagccg cgacgccgca tgatgcagga
    17581 ctgggccgat cgcctcgacc tgctcgaaca gggcgaagtg gaagccgcga gcgtgcacct
    17641 gaccatccgt atcgacgggg tgccggcgat ggcggaagtg gaggaagcga tgagtgcggt
    17701 ccccacggtg gccaagcccg ctgtccctgg cgtgccgcca gtggtggcca cgcccatcgt
    17761 cgtgaccccg aatagcggcg gaatcacgtt ccaacggctg tcgcaggtgc cgcagcctcc
    17821 ggtgcatgtc ccggagccgg aggtgtccgc gatccagcgc gagcgcgagg aaatgctggc
    17881 catctacgag tcgccgaaca atctgccggt cccgctgttc ggcaagctgg ccggcaagtc
    17941 caaggaccag atcaaccgcg agctgaaggc gggcaagctg ctgtcgatca gcctgggcaa
    18001 ccgggggcag cgggttcccg actggcaact agtgccgctc aagcacaaac tggcccaagt
    18061 gctcatgaac caatgcccgc acgcgaattc ttggaagctg taccacatgc tgacccagcc
    18121 tcaccccgac ctgggtgatc gtgcggctat cgatgcggtc acgccgacta acgtgcccgc
    18181 gatcatccgc gtcataatgg gtgactacca gcgccacgag gatgggcccg aggccgttgc
    18241 cttgcggccc attcccgagg atgtgcggct gagcgtccgt cggctagtgg acagcgcggc
    18301 agtacttgag ggggcttaac cccattcaaa ctacggccgg ggttatagct ccggccatgg
    18361 aggcggcgcg ccttccttgg tccagccaca tcacagatcc agatgttcag gccgttgggt
    18421 tgcttgcgat gcacttgcag tcgttcgaat cgtttttgca cccattacgg tcgcgcgcca
    18481 gatggccttg ccgtcgagcg tcggctgcaa catgccgtgg tcctggagca cattgaacac
    18541 tgcggtgttg ttcgcaggga tgccatcgac accctgggac agcaggtgcg cgcgcagctt
    18601 gtccgatacc gtcttgctca ccagccacag cgcgtcctcg gtgagccagc catcggaggc
    18661 ttcgggctgg ttcagtttca gctcttcctt cagcaggtag cgcagcccgt cgaccagctt
    18721 gcgttggagc gagtgcttgg gcgcggccat ggcgcgcgcg ggatcgccgc ccagttcctg
    18781 ggccacggat gcgcggtcgg cttgcacgac cagctcgccc agcaccccgg cgtgctcgta
    18841 ctgcccagcc aggacgtaga gcagcggcgc ccacagggat cgatagccac tgagccagtc
    18901 cagcagatcg cgatcgagca attgccggta gagcaagcct gttgcggcgc tatggagccg
    18961 atactcgcgg tcgtcgcggt agcggaagcg gtacggttgg cgcagcgggc cgtgccatgg
    19021 gtgccaggtg ctgccgtcgg ccagctcgac gtgcagatcg acggccacct tgccgatgtc
    19081 atgaagcagt gcggcatagg cgacggccgc agtccaggct tcggcctgcg ccgcctggtc
    19141 ttcggggttg gcgccgatgg gcagcagatg ggactgccgc agtttcatgc tgtaggcgac
    19201 gatttccagg ccgtggtcga gcatgccgcc cggataggcg tggtggtgcg cttcggaggc
    19261 cgggaaagcc tggaccagct cggcgtagcg ctccagcggg gcgcggtaga gggtggcgaa
    19321 ctgccggcgc gagagcgacg tgcgctgcca gatgtgttcc agcagcttct gccggcgcgg
    19381 ggtggccagc agcgatgcgg ccgactccgg ccgcatcaac cctttcggga gatcgacggc
    19441 gggagctggt gacggcgcgg cagcgaccgg gggccgtttt cgctggaaca gggagagcat
    19501 gtcggtgtcc tgatggcggg ccgggcggga ggcctttttg ccttttcgag gtagggcctt
    19561 tccccttgca ccccattccc ttgccgtttc gaccctttgg cctttaaccg tttcggtata
    19621 gcggggcctg ggttgcggct ccagcgtcaa tgtggaaccc gcacgaatgg attgggcggc
    19681 gacccggcat cggccccggc ctatgctgcc aagacgctac caaggtaggc ttccactaag
    19741 gcgacaaagc tgacaagagc gacaaaggcg actatagtga accctgtctc aaacctacct
    19801 gggagctgac tatgcccact gccatcgaat tcattgccga ccgtcttccg cgcgtcacgg
    19861 tggaggatgt gcgccgcttc gccgataccg tcgaaatccg ggatgcccca gcgttcgcgg
    19921 ccgagttgca ggcgttcatt cacgagcgcg tggaagcggt gacgctgccc gccaacctcg
    19981 aaggcgaaac ggtggcgcag gccttggagc gcaaggcggc cgcgctgcgc gccgacacgc
    20041 gctgggcacc gactgaaacc gacgtccagc gaggccgcgc cgcgctgctg gcagccttta
    20101 accagccgga caacctgccg cctgccgagt tcgccaagct ggccgacaag tcgcgccagc
    20161 aaatctacaa ggacatcctc gcgcgccggc tgctggcgct gaacgtgggg ccgcgcggcc
    20221 agaagctacc cgactggcag ctcgacccgg tgaagcagca gttgacccaa accgtgctcc
    20281 aggaggtcga gggcatcgac cactggacga tctaccgcgc gctgtccgaa ccgctcgaag
    20341 gcttgggcgg tcgctcgccg gtggatgcgg tgacgcatgg cacgatcgac gacgtggccg
    20401 aggccgtgtt caacgtgctg ggcgttcagg tgcattgaag cgagatcgcc atgagccacg
    20461 agctgccttc gttcctgatc gatgccggtg aactgctcca gcatgtgagc cgcgtcgtct
    20521 atcggggcag cccgctgtac tatggccgca gcagcaccaa tcgctacgat gacccggccc
    20581 aggcctacgg ggtgctctac ctggggcgcg acctgcccac ggcgctgatg gagtcggtgt
    20641 ttcataagca ccagtggctt gcggacacga agcgctcgat cgcgctgaaa gaagtccaga
    20701 gccggatggt gcgcgcagtg ggcgtgatgg acgacgtgct tctggccgac ctcacggcgc
    20761 cgggcgtcat ggcgggctac ttcggcctga acctggagca gttggccagc cgcgactaca
    20821 cgcacacgca gcgggtgtcc gcccaggtgc atgcgatgct cggagatgac ggccaggcgc
    20881 tgttcgacgg ggtgctctat ccgtcgcgca acaactatcc cgccaagagc atcgccctgt
    20941 tcgagcgtgc gggagcgaag gttggcgtca tcgaggacat cgacatggtg gaccatgtgg
    21001 actggccgcg gttcgttgcc acctaccgca tcggcgtgga acccgatccc ggcccggtgg
    21061 agccggagga cgaagcgtcc tgaagcagca agaaagcgca ttgaagcctc aaaccgaatg
    21121 aagtggagca aactggaata ggcattcctc aacagcagga gacgaccatg aacacgacga
    21181 cccgcaccag caccgcagaa cgtctcggcc gcgcctttgg ccgcggatgg cgtgcctatg
    21241 cgcgcggcga acggcgggcg tcgaactggt tggtgtccaa gggcatgccg cgagctggcg
    21301 ctaccgcgct cgtgtgggta gccgccacca ttggcaacct gcgcgaggcc agcatcgcgg
    21361 gtgtcgtcgt agg
//
LOCUS       DAOOOG010000024        20982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_2494_length_20982_cov_29.208439, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000024 DAOOOG010000000
VERSION     DAOOOG010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 20982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20982
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2494_length_20982_cov_29.208439"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="PK242_03445"
     CDS             <1..111
                     /locus_tag="PK242_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOZ93102.1"
                     /translation="GGCCGTTPEHIGAIARAVGPLAPRGVQAAGFYKEAA"
     gene            complement(208..1089)
                     /locus_tag="PK242_03450"
     CDS             complement(208..1089)
                     /locus_tag="PK242_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ93103.1"
                     /translation="MTTLTRRQLAGLLLLTLMWGLNWPIMKLSLRELAPLHFRALTMG
                     LGALVLAAYFAARGLRLWPRGTAEWRDVLVLGLPNILGWHALSIIGVAHLPAGRAAIL
                     GFTMPVWTVLLSVLFYGERLSARLLLAVGAVLAGITLLLWGELAQLAGRPAGIAWMQG
                     AALAWALGTIWMRRAHLTLPAESLVVWMMAWSALVLALLAWWLEPPQRASLSAAVWLS
                     LLWSVLINYGAAQVIWFALARALPAATSAMSVMAVPLVGTLSAPLIVGEWPGWHDLAA
                     MVCVVVAIGTVLLRPNR"
     gene            complement(1127..2077)
                     /locus_tag="PK242_03455"
     CDS             complement(1127..2077)
                     /locus_tag="PK242_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOZ93104.1"
                     /translation="MKTVTFRQLRVFVEVARHLSFVRAAEALHLTPPAVTMQIKELES
                     AIELPLFNREGRKVSLTTVGEYFLVYAKRLLATLKDADDAMARFRKLESGALSIGLVS
                     TAKYFVPQLLTRFRDEHPGVELKLLVGSNREQLVAMLQQGDVDLAIMGRPPKEMATRA
                     EPFAAHPHVFVAPPRHPILAVSQPSLAALARFPLIVREAASGTRALMDRFFQDHRVEP
                     RIAMEMPSNETIKQAVMAGMGLSFLSLHTIGLELRCGLLERVPVEHTPLVRTWNVVHM
                     QSKLLSPAAEALRYFILEHGEAHLAAHDRALLAADSAPSV"
     gene            2170..2865
                     /gene="rpe"
                     /locus_tag="PK242_03460"
     CDS             2170..2865
                     /gene="rpe"
                     /locus_tag="PK242_03460"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOZ93105.1"
                     /translation="MDHRIAPSILSADFARLGEEVRDVIAAGADWIHFDVMDHHYVPN
                     LTMGPAVAQAIKPHCKRADGTPVPLDVHLMVQPVDALAAAFAQAGADLITFHPDASTH
                     VDRTLQLIRAAGCQAGLAFNPAAPIDVLEWVIDRVDLVLVMSVNPGFGGQTFIDGTLP
                     KLRRLRQWIDAEHERSGRTIRLEVDGGIKADNIRRVADAGADTFVAGSAIFGQPDYRA
                     VIERMRAELERAR"
     gene            2910..3998
                     /locus_tag="PK242_03465"
     CDS             2910..3998
                     /locus_tag="PK242_03465"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOZ93106.1"
                     /translation="MPLSERQTLTQYLIEQRRRFPGASGELNALLLDVAIACKGIARA
                     VAQGELGADPASANADAGDVNVQGEVQKRLDVISNQMFLRQTEWSGNLAGMASEEMAQ
                     PYPIPARYPRGKYLLVFDPLDGSSNLDVNGAVGSIFSILRAPPEVVGAHRDLTEADFL
                     QPGTCQVAAGYALYGPVTMLVLSVGSGVVGFTLDAHIGEFKLTHPNIAVPEDANEFAI
                     NTSNSRFWEPPVKRYIDECLAGRSGPRGKDFNMRWIASMVAEAHRILMRGGVFMYPRD
                     TKDPSKPGRLRLLYEANPIGFIMEQAGGRASTGRQNVLGVAPSSLHQRIGLVFGSRNE
                     VERIERYHQEPARKAPAPLFAERSLFRE"
     gene            4024..4908
                     /locus_tag="PK242_03470"
     CDS             4024..4908
                     /locus_tag="PK242_03470"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HOZ93107.1"
                     /translation="MSERNPIIAITGSSGAGTSSVTRTFENIFRREGVTAAVIEGDSF
                     HRYDRKEMKARQAEAERNGNKHFSHFGPENNLFSELEQLFRSYSETGTGRRRKYLHDP
                     VEAAPYQQEPGTFTPWEPLPEGTELLFYEGLHGAVVTPEVNVARYPDLLIGVVPVINL
                     EWIQKLYRDKNVRGYSAEAVTDTILRRMPDYVNYICPQFAHTHVNFQRVPVVDTSNPF
                     IARDIPAADESICVIRFANPKGIDFPYLLNMIHNAWMSRANTIVVPGGKMDLAMQLIF
                     TPFIWRMMERRRHAMGGL"
     gene            4908..6950
                     /gene="tkt"
                     /locus_tag="PK242_03475"
     CDS             4908..6950
                     /gene="tkt"
                     /locus_tag="PK242_03475"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOZ93108.1"
                     /translation="MAAHATERVVRGSAGCSDPRANALRALAIDAVQQASSGHPGAPM
                     GMAEMAVALWARHLRHSPANPQWPNRDRFVLSNGHASMLLYALLHLSGYDLPVEEIRR
                     FRQLHSRTPGHPEVGITPGVETTTGPLGQGIGNAVGMALAEQMLAREFNRPGHTIVDH
                     HTYAFVGDGCLMEGVSHEACALAGVWGLGKLIALYDDNGISIDGPVHGWFRDDTPSRF
                     RAYGWEVIGPVDGHDIGAVEQAIAQARAGADRPTLIVCRTTIGQGSPARAGTARAHGE
                     PLGAEEVVATRAAIGWTADAFDVPPAVRAQWDATTAGRAHEAAWQAKFDAYRDAHPAL
                     AAEFERRVRGELPATFGATLHAAIETLGATTDKVASRKASQQVLGLLAPKVPEMFGGS
                     ADLTGSNLTDFPGADQRHLSYGVREFGMAAIMNGVALHGGFIPYGGTFLTFSDYSRNA
                     IRMAALMKQRVIHVFTHDSIGLGEDGPTHQAVEHVPALRLIPHLDVWRPADNVETAVA
                     WGAALQRRDGPTVLALSRQGLPQLTRPTQAEAMARGGYVFAEPPQELPAQAVIIATGS
                     ELQLARQAQQLLWAEGLPARVVSMPSTSVFDRQDAAYREHVLPSQLPTVAVEAAHPDF
                     WRKYVGRRGGVLGVATFGESAPAEDLYRHFGLTAERVAAAVRACVRAAAPVGGAGS"
     gene            6950..7615
                     /gene="gph"
                     /locus_tag="PK242_03480"
     CDS             6950..7615
                     /gene="gph"
                     /locus_tag="PK242_03480"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548220.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HOZ93109.1"
                     /translation="MRAVLFDLDGTLMETAPELTDALNDTLRRHDLPAVDEARVRGWI
                     GDGARALLAKAFAHAGATPAAANAAWPGFHRDYSARCGTRSSLFPGARELLARLSKAG
                     IRTALLTNKESAFAHRLLARHDLASAFDLIVAGDTLAVKKPDPGVVMHALQKLHIEPG
                     QALLLGDSLTDVRTARAAGIAVRIVRHGYPQGELAGADAPDAFIDHFDDFDPGACRHA
                     PAT"
     gene            7643..8680
                     /gene="fba"
                     /locus_tag="PK242_03485"
     CDS             7643..8680
                     /gene="fba"
                     /locus_tag="PK242_03485"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348752.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HOZ93110.1"
                     /translation="MALVSLRQVLDHAAEHGFGVPAFNVNNLEQIQAIMEAAQATDSP
                     VILQASAGARNYAGEAYLRHMVLAAVETHPDIPVVLHQDHGASPAVCMQAIRSGFTSV
                     MMDGSLQEDARTPASYAYNVDVTRRVCAMAHAVGVSVEGELGCLGSLETGEAGEEDGS
                     GAQGKLSHDMLLTDPAQARDFVAATGVDALAIAIGTSHGAYKFSRQPTGDMLAIDRIA
                     SIHAAIPHTHLVMHGSSSVPQEWLAIIRKHGGTIKETYGVPVEEIQRGIAHGVRKINI
                     DTDIRLAMTGAMRRLFAEQPAEFDPRKAMAEARRAAKGICQARFEAFGCAGHAGRMRP
                     IPLDAMALRYR"
     gene            8812..9558
                     /locus_tag="PK242_03490"
     CDS             8812..9558
                     /locus_tag="PK242_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOZ93111.1"
                     /translation="MLRALIWDVDGTVAETEADGHRIAFNQAFEACGLDWHWDLPTYG
                     QLLEVTGGRERLLAYMDRRDDAPASPAGRAALARAVHLRKNAFYADLVARGGIPARPG
                     VRRLMDECQRQEVRLAIATTTSTSNVEALFSSLFGPTWRDRFAAIVCAEDAPRKKPDP
                     QAYLLALRRLGVTPQEAFAIEDSPNGLRAARAAGLRCGITCSRYFADAGFEGAAWLRP
                     DLETPRPMTLQTLQALDGAAESETRPRGRP"
     gene            complement(9628..11958)
                     /locus_tag="PK242_03495"
     CDS             complement(9628..11958)
                     /locus_tag="PK242_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93112.1"
                     /translation="MSQTARFDAYGKAPPPPAAAASRHAALRERLDCRFPAIDDVFDD
                     CIDEALRTLTPAGMAAYLDQARWFGKLGRGAQPVLQFLAAWPAVARCCGESSLAPLCA
                     AAAAFQKSPNGPAIVTLLHSLPAVARQLPAPHLLAQYLALTVELMQRTSLSIHGRHAT
                     EPSPGLPAFLQQAGVLVALVPMEGLRRWVQEGIRLHGAHPQRQIDYFSLTSADARALL
                     QRERRGTLLIDVERRLALGLRGLWDVALPLVPYPVHVDHPTGQPLLPHLQDDGLRLPE
                     VLDDRAGVSALQRYRAMLAHLAGHLRWSTPQLADNLSPLQRLALECFEDARVDHLVLR
                     RYPGLRPALLALHPMPAEDACDAARVSCLRHRLVCLSRALLDPAHPYRDATLLAFAGR
                     FRAALRDGVSSTREMAVLALDWSAATRRASDQRARVQFEDTLVDWRDDNRHLWTFIEP
                     GDEEDTGAEPRRRAPVADEGGLPPRLYPEWDHLTRGHRPDWVSVYDILQPPGQAGDVD
                     ALLAQYAPVARHLKRRIDLLKPQDRTRLRHQEEGTELDLDLALRAIADWRAGAQPDPR
                     IHTSTRTDGRDISVLLLMDLSQSVNQTVGQPHTGAPAGGAPTVLSLSRAAAALLGWAI
                     AALGDELAIAGFQSNTRQEVRYLHLKGFTEAWGDAPKARLAAAQGAWSTRMGAALRHA
                     GHLLSGRRADKRLLLVLTDGEPADIDVDDPQYLIADAAQAVRELDRQGLYTHCISLDA
                     RADAYVSRIFGQRWTVIDRVEQLPRRLPELFLALTR"
     gene            complement(12105..12908)
                     /locus_tag="PK242_03500"
     CDS             complement(12105..12908)
                     /locus_tag="PK242_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HOZ93113.1"
                     /translation="MNPLADWRVAAEPFYAPQGGEIELFEAAYAARLPVMLKGPTGCG
                     KSRFVEHMAWKLQRPLVTVACNEDMTAADLVGRFLLEPDGTRWQDGPLTTAARHGAIC
                     YLDEVVEARQDTTVVIHALTDHRRALPLDKKGELVRAHADFQLVVSYNPGYQSLMKDL
                     KTSTRQRFAALDFDYPEAALEARIVVAETGIDAAVAAALVQVAGAARRLKGHGLDEGI
                     STRLVVYAATLIRQGIDARAACRMAMVRPITDDTDIRESLDHAIDAVFE"
     gene            complement(13031..14413)
                     /locus_tag="PK242_03505"
     CDS             complement(13031..14413)
                     /locus_tag="PK242_03505"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase"
                     /protein_id="HOZ93114.1"
                     /translation="MDQSNRYADLSLREEDLIRGGKHILVAYKMRPKAGHGYLAAAAH
                     FAAESSTGTNVEVSTTDEFTKGVDALVYLIDEVSEEMRIAYPLDLFDRNLIDGRMMIV
                     SFLTLAIGNNQGMGDIEYAKMYDFWVPPRALQLFDGPSKDISDLWRVLGRPLKDGGYI
                     AGTIIKPKLGLRPEPFAQAAYEFWLGGDFIKNDEPQGNQVFAPMKQVMPLVRDAMKRA
                     MDRTGEAKIFSANITADDHSEMLARGEYILETFGPDADKVAFLVDGYVGGPGMVTTAR
                     RWFPNQYLHYHRAGHGAVTSPQSKRGYTAYVLAKMSRLQGASGIHVGTMGYGKMEGEK
                     DDRAIAYIIERDSYTGPAYHQEWLGMRPTTPIISGGMNALRLPGFFENLGHGNVINTA
                     GGGSYGHIDSPADGARSLRQAYECWKAKADPIEWAREHREFARAFDSFPGDADKLYPG
                     WRDKLAGVHR"
     gene            complement(14610..15851)
                     /locus_tag="PK242_03510"
     CDS             complement(14610..15851)
                     /locus_tag="PK242_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HOZ93115.1"
                     /translation="MHPSGPHPLWFCLALALPAMASAQSPAMPAGAERIDAATAWQAC
                     LGQPDAQRLACFDGWAHGQRQLIEAIAQKAQSVEATPTRNAAPSAQAAAVRGELAQAL
                     ATTQPERADGALVSGSAGIVGVGLEQGCRDRQFSDLSRFWELESGSSCPTFGLRAFHP
                     TTASIAAGDHINRLPTSPNPANSAITPIDYGKREMRLALSVRTKLAGGLLTPAGGTLR
                     DSLWAAYTQQSYWQVFNGSLSRPFRNTDHMPELIYVYPTTLKLPGGWLWRYSGIGLAH
                     QSNGQSDPLSRSWNRAYLMAGFERGNALNLQLRLWKRLPEDSAKDNNPDIVRHIGRGD
                     LSLAWNVDAKNTLRATYTGSFNRYGSGRLEWTRSLGDGWGNSFSSLRLYTALFHGYGD
                     SLLDYNFKRTVFSIGLSLVDF"
     gene            16109..16552
                     /locus_tag="PK242_03515"
     CDS             16109..16552
                     /locus_tag="PK242_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HOZ93116.1"
                     /translation="MTPSPLLFPLQGALRRVLYVGLYEALAIAVVTVAFVAGDRSLGS
                     ASGVAVGSSLIAVLWNLVFNAAFERWESRQRVRGRSRARRLAHAIGFEGGLALWLVPF
                     MAWWLEVGLWQALLLDLGLLLFFLAYTFVFTWVFDRLFGLPRAAA"
     gene            16565..17770
                     /locus_tag="PK242_03520"
     CDS             16565..17770
                     /locus_tag="PK242_03520"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HOZ93117.1"
                     /translation="MLELADIQQAAERVRGQVLDTPCVESRTLSQLTGTQLFLKMENL
                     QFTASFKERGACNKLAQLTDQEKARGVIAMSAGNHAQGVAYHAERLGIPATIVMPSFT
                     PGVKVARTRGFGARVILHGDSLEAAYQHARTLAEAQNLVFVHPYDDLAIMAGQGTLAL
                     EMLAAQPELDTLVVPVGGGGLISGIATAAKALRPGIRVIGVQTQRYPAMVNAVQGTRH
                     AQGGNTIAEGIAVGAPGAHCLEVIRQRVDDLLLVDEGDIEQAIVLLLEIEKTLAEGAG
                     AAALAALLRHGERFAGRKVGMVLSGGNIDPQLLAQIIGRGLVRSGRLIRIVISARDVP
                     GSLARITALVAEAGANVDEVHHQRAFTLLAAQNVEIEMVLQTRGHEHVQELLQRLRGA
                     GLEVRLATA"
     gene            17870..18019
                     /locus_tag="PK242_03525"
     CDS             17870..18019
                     /locus_tag="PK242_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93118.1"
                     /translation="MPPPPSAPQAPHRHPPLLLRSAGWRLGVAAALGALLLLLWRWAL
                     AGASH"
     gene            18035..18823
                     /gene="aztA"
                     /locus_tag="PK242_03530"
     CDS             18035..18823
                     /gene="aztA"
                     /locus_tag="PK242_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HOZ93119.1"
                     /translation="MSAPILLHDLTLGYDGHPAVHHVSGVIEAGALLAVVGPNGAGKS
                     TLIKGLAGLLKPLGGRIEGLAGQRVAYLPQQATLERGFPVTVGEFAAMGLWRECGAFG
                     GFSPAQRQRVREALAQVGLEGHARQPIDTLSGGQLQRTLFARLMLQDADVLLLDEPFA
                     AIDQRTTDELLALLRQWHAGGKTVLAVLHDLHQVRASFPRTLMLARELRAWGDTADVL
                     TPANLQGLRVMPEAFDEHAPVCEAAPGHDHSHHGRAHHHAEHDR"
     gene            18889..19716
                     /locus_tag="PK242_03535"
     CDS             18889..19716
                     /locus_tag="PK242_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOZ93120.1"
                     /translation="MRRALVGCLALSLGCAPVGVFLLLRRMSLTGDAMAHAILPGAAI
                     GYLLSGLSLTAMSLGGVAAGLLVAVGSGLVARSTVLREDASLAAFYLLSLALGVLIVS
                     TRGSSVDLLHVLFGSVLALDDAALGLLVGIALLTLATLALLYRPLVLECLDAQFLRSV
                     SRFSPVAHYGFLVLVVINLVAGFHALGTLMAVGIMVLPAATARLWVRGVPALMALAAG
                     LALAACVVGLLLSYHLDWPTSPTIVLCLGGVYLLSLVAGRFGAWRQGQHGRQRHLRG"
     gene            19811..20728
                     /locus_tag="PK242_03540"
     CDS             19811..20728
                     /locus_tag="PK242_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOZ93121.1"
                     /translation="MRRHLVRATLALLTALPALAQGAAPLRVVASFSLLADMVRQVGG
                     DRVTVSALVGPGADAHVFQPTPAQASQVGQAQLVVTNGLGYEGWLPRLLRSAGYRGPQ
                     VVAARGIEPLPATPGHGHGGHGRDAHGHTDPHAWQSVPHAMVYVKNIADGLCAADPAG
                     CADYRAGAARYSAELKALDAEIRAAWAPIPAPRRLVITAHAAYAYYGAAYGVRFLAPQ
                     GVSTDSEASARGVAQLVRQIRQQGIRALFIENIADPRLIEQIARETGVRPAGRLYADA
                     LTEADGPAPSYVALMRANTRALTQAVGGP"
     regulatory      20742..20840
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
BASE COUNT         3252 a         7326 c         7279 g         3125 t
ORIGIN      
        1 ggcggctgct gcggcaccac gcccgagcac atcggcgcca tcgcccgcgc cgtggggccg
       61 ctggcgccgc gcggggtgca ggcggcgggg ttttacaagg aagcggcctg aagggcgggc
      121 gccccggtgc ctggggctcg ctgaattttt tgtgaaacag ggcgtcaacc cttgctggat
      181 attcccgatc agctcttgat tttgtagcta tcggttgggc cgcagcaaca ccgtgccgat
      241 ggccaccacc acgcagacca tcgccgccag gtcgtgccaa ccaggccatt cgcccacgat
      301 cagcggggcg ctcagggtgc cgaccagcgg caccgccatc acgctcatgg cgctggtggc
      361 cgcaggcagg gcgcgcgcca gcgcaaacca gatcacctgc gccgcgccgt aattgatcag
      421 caccgaccac agcaggctga gccagaccgc ggcgctcaag gatgcccgct gcggcggctc
      481 cagccaccat gccagcagcg ccagcaccag cgccgaccag gccatcatcc acaccaccag
      541 cgactcggcc ggcagggtca gatgcgcgcg ccgcatccag atggtgccca aggcccaggc
      601 cagcgctgcg ccctgcatcc aggcgatgcc cgccgggcgg ccggccaact gggccagctc
      661 gccccacagc agcagggtga tgcccgccag cacggcgccc accgccagca gcagacgcgc
      721 gctgaggcgc tcgccgtaga acagcaccga cagcagcacc gtccacaccg gcatggtgaa
      781 gcccaggatg gcggcccgac cggcgggcag atgggccacg ccgatgatcg acagcgcgtg
      841 ccagcccaga atgttgggca gacccagcac caggacgtcg cgccactcgg cggtgccgcg
      901 cggccacagg cgcaggccac gggcggcaaa gtaggccgcc agcaccagcg cacccaggcc
      961 catggtgagg gcacggaagt gcagcggcgc cagctcgcgc agcgacagct tcatgatggg
     1021 ccagttcagg ccccacatca gggtcagcag cagcaggccg gcgagctggc ggcgcgtgag
     1081 ggtggtcatc ggcgccagct taccgagccc agcaggcagc ccgggttcac accgatggcg
     1141 cagagtctgc cgcgagcagg gcgcgatcgt gcgcggcaag atgtgcctcg ccatgctcga
     1201 ggatgaaata gcgcagcgcc tcggctgccg gcgacagcag cttggactgc atgtgcacca
     1261 cgttccaggt gcgcaccaac ggcgtgtgct cgaccggcac ccgttccagc aggccgcagc
     1321 gcagctccag gccgatggtg tgcagcgaca gaaagctcag gcccatgccg gccatcaccg
     1381 cctgcttgat ggtctcgttg ctcggcatct ccatggcgat gcgcggctcg acccggtggt
     1441 cctgaaagaa gcggtccatc agtgcgcgcg taccggaagc ggcctcgcgc acgatgagcg
     1501 ggaacctggc cagcgccgcc agcgatggtt ggctgacagc caggatggga tgtcgcggcg
     1561 gtgcgacaaa gacatgcggg tgcgcggcaa agggctcggc gcgcgtggcc atctccttcg
     1621 gtgggcggcc catgatcgcc agatccacgt caccttgctg cagcatcgcc accagctgct
     1681 cgcggttgct gcccaccagc agcttgagct cgacgccggg gtgttcgtcg cgaaaacgcg
     1741 tcagcagctg cggcacgaaa tacttggccg tgctgaccag tccgatcgac aaggcccctg
     1801 attccagctt gcgaaagcgc gccatcgcat cgtcggcgtc cttcagcgtc gcgagcaacc
     1861 gcttggcgta gaccagaaag tactcaccga ccgtggtgag cgacaccttg cgcccctcgc
     1921 ggttgaacag cggcagctcg atcgccgact ccagctcctt gatctgcatc gtcaccgccg
     1981 gcggcgtcag atgcagcgcc tcggccgcgc gcacgaagct caggtgcctg gccacttcga
     2041 caaaaaccct gagctgtcgg aaggtgacgg tcttcattca gggattctga atcgaaagcc
     2101 aaagatatgt gaatttacca taagcatggt gccgcctaca ttgaaaaaaa gacgcatgcc
     2161 gcagcaccca tggaccatcg cattgccccg tcgatccttt ctgccgattt cgcccgcctg
     2221 ggcgaggagg tgcgcgatgt catcgccgcc ggtgccgact ggatccactt cgatgtgatg
     2281 gaccaccact acgtgcccaa cctgaccatg ggcccggctg tggcgcaggc catcaagccc
     2341 cattgcaagc gtgccgacgg caccccggtg ccgctggacg tgcacctgat ggtgcagccg
     2401 gtcgacgcgc tggccgctgc ctttgcccag gccggcgccg acctgatcac cttccatccc
     2461 gacgccagca cgcacgtgga ccgcaccttg cagctgatcc gggcggccgg ctgtcaggcc
     2521 gggctggcgt tcaacccggc ggcacccatc gatgtgctgg agtgggtgat cgacagggtc
     2581 gatctggtgc tggtgatgag cgtcaacccc gggttcggcg ggcagacttt catcgacggc
     2641 acgctgccca agctgcgacg gctgcgtcag tggatcgacg ccgaacacga acggtccgga
     2701 cgcacgatcc ggctggaagt ggatggcggc atcaaggccg acaacatccg ccgcgtggcc
     2761 gatgcggggg ccgacacctt cgtggccggc agcgcgatct tcggccagcc cgactaccgc
     2821 gccgtcatcg aacgcatgcg tgccgagctc gaacgcgccc gctgaccgca cccgaacccc
     2881 accgacgcga caccccgagg aagtccgcca tgccgctgtc cgaacgccag accctgaccc
     2941 agtacctgat cgagcagcgg cgccgctttc cgggtgccag cggcgagctc aacgcgctgc
     3001 tgctggatgt ggcgatcgcc tgcaaaggca tcgcacgggc ggtggcccag ggcgagctgg
     3061 gtgcggaccc ggcaagcgcg aacgcggatg ccggcgacgt caacgtacag ggcgaagtgc
     3121 aaaagcgcct ggacgtgatc tccaaccaga tgttcctgcg tcagaccgag tggtcgggca
     3181 acctcgcggg catggcctcc gaggagatgg cgcaacccta cccgatccct gcgcgctatc
     3241 cgcgcggcaa gtatctgctg gtgttcgacc cgctcgacgg ctcgagcaac ctcgacgtga
     3301 acggtgccgt cggcagcatc ttctcgatcc tgcgcgcgcc gccggaggtg gtcggcgccc
     3361 accgcgacct caccgaagcg gactttctgc agcccggcac ctgccaggtc gccgccggct
     3421 acgcgctcta cgggccggtg acgatgctcg tgctcagcgt gggcagtggc gtggtcggct
     3481 tcacgctcga cgcccacatc ggcgagttca agctcaccca cccgaacatc gcggtgcccg
     3541 aggacgccaa cgagtttgcg atcaacacct ccaacagccg cttctgggaa ccaccggtca
     3601 agcgctacat cgacgagtgc ctggccggca ggagcgggcc gcgcggcaag gacttcaaca
     3661 tgcgctggat cgccagcatg gtggccgagg cgcaccgcat cctgatgcgc ggcggtgtct
     3721 tcatgtaccc gcgcgacacc aaagacccga gcaagccggg ccgcctgcgc ctgctgtacg
     3781 aggccaatcc gatcggcttc atcatggaac aggctggcgg tcgtgccagc accggccgcc
     3841 agaacgtgct cggcgtggcg ccgagcagcc tgcatcagcg catcggcctg gtgttcggct
     3901 ccaggaacga agtcgagcgc atcgaacgct accaccagga acccgctcgc aaggcgccgg
     3961 cgccgctgtt cgccgagcgc agcctgtttc gcgagtgaag gcgtacacga cacaggagag
     4021 tccatgtccg aacgaaatcc catcatcgcc atcaccggct cctcaggggc aggcacgtct
     4081 tcggtgacgc gcaccttcga gaacatcttc cgccgagagg gcgtgaccgc cgcggtcatc
     4141 gaaggcgaca gcttccatcg ctacgaccgc aaggagatga aggcacgcca ggccgaggcc
     4201 gagcgcaacg gcaacaagca tttcagccac ttcggcccag agaacaacct gttctccgaa
     4261 ctcgaacaac tgtttcgcag ctacagcgaa accggcaccg gccgccgccg caagtacctg
     4321 cacgacccgg tggaagcggc gccgtaccag caggaacccg gcaccttcac gccctgggaa
     4381 cccctgcccg aaggcaccga gctgctgttc tacgaaggtc tgcatggcgc cgtggtcacg
     4441 ccggaagtca acgttgcccg ctacccggac ctgctgatcg gtgtggtgcc ggtgatcaac
     4501 ctggagtgga tccagaagct ctaccgcgac aagaacgtgc gcggctactc ggccgaggcg
     4561 gtgaccgaca ccatcctgcg ccgcatgccc gactacgtca actacatctg cccgcagttc
     4621 gcgcacacgc acgtcaactt ccaacgcgtg ccggtggtcg acaccagcaa ccccttcatc
     4681 gcacgcgaca ttccggccgc cgacgagagc atctgcgtca ttcgcttcgc caacccgaag
     4741 ggcatcgact ttccgtacct gctcaacatg atccacaacg catggatgag ccgcgccaac
     4801 acgatcgtgg tgccgggcgg caagatggat ctggccatgc agctgatctt cacgcccttc
     4861 atctggcgca tgatggagcg gcggcgccac gccatgggag gactgtgatg gcggcccatg
     4921 ccaccgaacg tgtcgtccga ggcagcgcgg gctgcagcga cccacgcgcc aacgcgctgc
     4981 gtgcgctggc catcgacgcc gtgcagcaag ccagctccgg acaccccggc gcaccgatgg
     5041 gcatggccga gatggccgtg gcgctgtggg cccgccatct gcgacacagc ccggccaacc
     5101 cgcagtggcc caaccgggat cggttcgtgc tgagcaatgg ccatgcgtcg atgctgttgt
     5161 atgcgctgct gcacctgagc ggctatgacc tgccggtgga ggagatccga cgctttcgcc
     5221 agctgcacag cagaacgccg gggcaccccg aggtcggcat cacgcccggc gtggaaacca
     5281 ccaccggccc gctcggccag ggcatcggca acgcggtcgg catggcgctg gccgagcaga
     5341 tgctcgcccg cgagttcaac cggccgggac acaccatcgt cgaccaccac acctacgcct
     5401 tcgtcggcga cggctgcctg atggaaggcg tcagccatga agcctgtgcg ctcgccggcg
     5461 tctggggcct cggcaagctg atcgcgctct atgacgacaa cggcatcagc atcgacgggc
     5521 ccgtgcacgg ctggttccgt gacgatacgc cgtcgcgctt tcgcgcctat ggctgggagg
     5581 tgatcggtcc ggtcgatggc cacgacatcg gcgccgtcga gcaggccatc gcacaggccc
     5641 gggcgggcgc cgatcgaccg accctgatcg tctgccgcac caccatcgga caaggctcgc
     5701 cagcgcgcgc cggcaccgcc cgggcgcatg gcgagccgct cggcgcggaa gaggtggtcg
     5761 ccacccgggc ggcgatcggc tggacggcgg atgccttcga cgtgccgccc gcggtgcgtg
     5821 cgcagtggga tgcgacgacg gcgggccgcg cgcacgaggc cgcgtggcag gcgaagttcg
     5881 acgcctaccg cgacgcccat ccggcactgg ccgccgagtt cgagcgccgt gtccggggcg
     5941 agctgccggc gacgttcggc gccacactgc acgccgccat cgagaccctg ggtgccacga
     6001 ccgacaaggt ggcttcgcgc aaggcctcgc agcaggtgct gggcctgctg gcgcccaagg
     6061 tgccggagat gttcggtggc agcgccgacc tcaccggatc gaacctgacc gactttcccg
     6121 gtgccgacca gcgccacctg agctatggcg tgcgcgagtt cggcatggcc gccatcatga
     6181 acggggtggc gctgcatggc ggcttcatcc cctacggcgg caccttcctg accttcagcg
     6241 actacagccg caacgccatc cgcatggcgg cgctgatgaa gcagcgcgtg atccacgtct
     6301 tcacgcacga ctccatcggc ctgggcgagg acggccccac acaccaggcg gtcgagcatg
     6361 tgccggcgtt gcgactgatc ccgcacctcg acgtctggcg cccggccgac aacgtcgaga
     6421 ccgccgtggc ctggggcgcg gcgctgcaac ggcgtgacgg ccccacggtg ctggcgctgt
     6481 cgcgccaggg gttgccgcag ctgacacggc ccacccaggc cgaagccatg gcgcgcggcg
     6541 gttacgtgtt cgccgaaccg ccgcaggagc tgcccgctca ggcggtgatc atcgccaccg
     6601 gctcggagct gcagctggca cggcaggcgc agcagctgtt gtgggccgaa ggactgccgg
     6661 cacgtgtggt gtcgatgccg agcaccagcg tattcgaccg ccaggacgcc gcgtaccgcg
     6721 agcatgtcct gccaagccag ctgccgacgg tggcggtgga agccgcacac ccggacttct
     6781 ggcgcaagta cgtcggccgt cggggcggcg tgctgggggt cgcaacgttc ggcgaatccg
     6841 cccccgccga ggatctgtac cggcacttcg gcctgacggc agagcgcgtc gccgccgcgg
     6901 tgcgcgcctg cgtccgggcg gcggcacctg tcggtggggc aggctcctga tgcgggccgt
     6961 cctgttcgac ctcgacggca cgctgatgga gaccgcaccg gagttgaccg acgcgttgaa
     7021 cgacacgctg cgccggcacg acctgcccgc ggtcgacgag gcgcgcgtgc gcggctggat
     7081 cggtgacggc gcgcgcgcgt tgctggccaa ggccttcgcg cacgccggcg caacgccggc
     7141 agcggcgaac gccgcctggc ccgggttcca ccgcgactac agcgcccgct gcggcacccg
     7201 atcgtccctg tttcccggtg cgcgcgagct gctcgcccgc ctgagcaagg cgggtatccg
     7261 gacggcactg ctcaccaaca aggaatccgc cttcgcacac aggctgctgg cgcgccacga
     7321 tctcgccagc gccttcgacc tcatcgtcgc cggggacacg ctggccgtga agaaacccga
     7381 tccgggcgtg gtcatgcacg cgctgcagaa gctgcacatc gaacccggcc aggcgctgct
     7441 gctcggcgat tcgctcaccg atgtgcgcac cgcgcgcgcg gcaggcatcg ccgtgcggat
     7501 cgtgcgccac ggttacccgc agggcgagct ggcgggtgcc gacgcgcccg acgccttcat
     7561 cgaccatttc gacgacttcg atcccggcgc atgcaggcac gcgcccgcga cctagttttc
     7621 aacgtcaacc caggagttcc caatggccct cgtctcactg cgccaggtgc tggatcacgc
     7681 cgccgaacac ggctttggcg tgccggcttt caacgtcaac aacctggagc aaattcaggc
     7741 catcatggaa gccgcccagg ccaccgactc gccggtgatc ctgcaggctt cggccggcgc
     7801 gcgcaattac gccggcgaag cttacctgcg ccacatggtg ctggccgccg tggaaaccca
     7861 tcccgacatc ccggtggtgt tgcaccagga ccatggcgcc tcgcctgccg tgtgcatgca
     7921 ggcgatccgt tcgggcttca cgagcgtgat gatggacggc tcgctgcagg aagatgccag
     7981 gacgccggca agctacgcgt acaacgtcga cgtcacacgc cgtgtctgcg cgatggcgca
     8041 cgccgtgggc gtgtcggtcg agggcgagct cggctgcctg ggctcccttg agaccggcga
     8101 agccggcgag gaagacggca gcggcgccca gggcaagctg agccacgaca tgctgctcac
     8161 cgacccggcg caggccagag actttgtggc ggccaccggc gtggacgcgc tggcgatcgc
     8221 catcggcacc agccacgggg cctacaaatt cagccgccag cccaccggcg acatgctggc
     8281 gatcgaccgc atcgccagca tccacgccgc cataccgcac acgcacctgg tgatgcatgg
     8341 ctcgtcgagc gtgccgcagg agtggctcgc catcatccgc aagcacggtg gcacgatcaa
     8401 ggagacctac ggcgtgccgg tcgaggagat ccagcgcggc attgcccatg gggtacgcaa
     8461 gatcaatatc gacaccgaca tccgcctggc aatgaccggc gcgatgcgca ggctgttcgc
     8521 cgagcaaccg gccgaattcg atccgcgcaa ggccatggcc gaggcccggc gggcggccaa
     8581 aggcatctgc caggcacgct tcgaagcgtt cggctgcgcc ggacacgccg gccgcatgcg
     8641 gccgattccg ctggatgcga tggccctgcg ctaccgctga acccgaaccc ggccgttgat
     8701 cgctggccag acgccggagc ggcatatggg gccacaccgc ttccctgacc ttcaccgggt
     8761 gggcggacgc gcgcggacga cccgcgaatc ggacagggag cacactgcgt catgttgcgc
     8821 gcattgatct gggatgtgga cggtacggtg gccgaaaccg aggccgatgg ccatcggatc
     8881 gcgttcaacc aggccttcga ggcgtgcggg ctcgattggc actgggacct gccgacttac
     8941 ggccagttgc tggaagtgac cggcggccgc gagcgcctgc tcgcctacat ggacaggcgc
     9001 gacgatgcgc cggccagccc cgcaggccgt gccgccctgg cacgcgcggt gcacctgcgc
     9061 aagaacgcgt tctatgccga tctggtggcg cgcggcggca tcccggcccg tcccggcgtg
     9121 cgccggctga tggacgaatg ccaacgccaa gaggtgcggc tggcgattgc caccaccacc
     9181 agcacgagca acgtcgaggc cctgttttcc agtctgttcg gcccgacctg gcgcgatcgc
     9241 ttcgctgcca tcgtctgcgc cgaagacgcc ccacgcaaga agcccgaccc gcaggcctac
     9301 ctgctggcgc tgcgtcggct cggcgtcacg ccccaagagg ccttcgccat cgaggactcg
     9361 cccaacggcc tgcgggccgc gcgagcggcc ggcctgcgct gcggcatcac ttgcagcagg
     9421 tatttcgccg acgccgggtt cgaaggagcg gcatggctgc ggcccgacct cgagacccca
     9481 aggcccatga ccttgcaaac cctccaggcg ctggatggcg ccgcagaaag cgaaacccgg
     9541 ccgcgcggcc gaccatgaac aagaagatcc cagccgctcg ggtgcccggg gcgcctgcgg
     9601 cgcgcgaggg gcctgttcgg catgacccta acgcgtcagg gccaggaaca gttcgggcag
     9661 ccgccgcggc agctgttcga cacggtcgat caccgtccag cgctggccaa agatgcggct
     9721 gacgtaggca tcggcccgcg cgtccagact gatgcagtgc gtgtacagac cttgccggtc
     9781 gagctcgcgc accgcctgcg cggcgtcggc gatcaggtac tgcggatcgt ccacatcgat
     9841 atcggcgggt tccccgtcgg tcagcacgag cagcagccgc ttgtcggccc tgcgtcccga
     9901 cagcaggtgt ccggcgtggc gcaatgccgc ccccatgcgc gtcgaccagg ccccctgcgc
     9961 cgccgccagg cgggctttgg gcgcgtcgcc ccacgcttcg gtgaacccct tcaggtgcag
    10021 gtagcgcacc tcctgtcggg tgttggactg aaacccggcg atggccagtt cgtcgcccaa
    10081 ggccgcgatc gcccagccca gcagcgccgc tgcggctcgg ctgagcgaca acaccgttgg
    10141 cgcgccgccg gcgggggcgc cggtgtgcgg ctgcccgacc gtctgattga ccgattgcga
    10201 cagatccatc agcaacagca ccgaaatgtc acgcccgtcg gtgcgtgtgc tggtgtggat
    10261 gcgcgggtcg ggctgggcgc cggcgcgcca gtcggcgatg gcgcgcagcg ccagatcgag
    10321 gtcgagttcg gtgccctctt cctggtggcg cagtcgcgtg cgatcctgcg gcttgagcag
    10381 atcgatccgc cgcttgaggt gccgtgccac cggcgcgtac tgtgcgagca gcgcgtcgac
    10441 atcgccggcc tggcctgggg gctgcaggat gtcgtacacg ctcacccagt cgggccgatg
    10501 gccgcgagtc aggtgatccc actcgggata caggcgcggc ggcagaccgc cttcatcggc
    10561 caccggagcg cggcgccgcg gctcggcgcc ggtgtcttcc tcgtcgcccg gttcgatgaa
    10621 ggtccacaga tggcggttgt cgtcgcgcca gtcgaccagc gtgtcttcga actgcacgcg
    10681 cgcccgctga tcgctcgcgc gtcgcgtggc ggccgaccag tcgagcgcca gcacggccat
    10741 ctcgcgggtg gacgacacgc cgtcgcgcag ggcggcacga aaacgcccgg caaaggcgag
    10801 cagtgtcgca tcgcggtacg gatgggccgg atcgagcagc gcgcgcgaca ggcagaccag
    10861 ccggtgacgc aggcacgaca cgcgggccgc atcgcaagcg tcctcggccg gcatggggtg
    10921 cagcgccagc agcgccggcc gcagaccggg gtagcggcgc agcaccagat gatcgacacg
    10981 cgcatcctcg aagcactcca gcgccaggcg ctgcagcggg ctcaggttgt cggccagctg
    11041 cggtgtgctc cagcgaagat gccctgccag gtgggccagc atggcgcggt agcgctgcag
    11101 ggcggaaact ccggcgcgat cgtccagcac ctcgggcagg cgcaggccgt cgtcctgcag
    11161 gtgcggcagc aggggctggc cggtcggatg gtcgacatgc accgggtacg gcaccagcgg
    11221 cagcgccaca tcccacagcc cacgcaggcc cagtgcgagc cgacgctcga cgtcgatcag
    11281 cagagtgccg cggcgctcac gctggagcag cgcgcgggcg tctgccgagg tcaggctgaa
    11341 atagtcgatc tggcgctgcg ggtgcgcgcc atgcagacgg atcccttcct gcacccagcg
    11401 ccgcaagccc tccatcggca ccagggccac cagcaccccc gcctgctgca gaaaggctgg
    11461 caggccaggg ctcggttcgg tggcgtggcg gccatggatg gaaaggctgg tgcgctgcat
    11521 cagctcgacc gtcaacgcca ggtactgcgc cagcagatgc ggcgccggaa gctgccgcgc
    11581 caccgccggc aggctgtgca gcagggtcac gatggcgggt ccgttgggcg acttctggaa
    11641 cgccgccgcg gccgcgcaca gcggcgccag cgaagactcg ccacagcaac gcgccaccgc
    11701 gggccaggcg gcgagaaact gcagcaccgg ctgggcgccg cgccccagct tgccgaacca
    11761 gcgcgcctgg tcgaggtagg ccgccatgcc agccggggtg agcgtgcgca gggcctcgtc
    11821 gatgcagtcg tcgaacacat cgtcgatggc gggaaagcga cagtcgaggc gctcgcgcag
    11881 tgccgcatgg cgcgacgccg cggccgccgg cggcggcggc gccttgccgt aggcatcgaa
    11941 gcgggccgtc tggctcatcg acagtcacac cgcgatggcg tgccgggctc gaccaggccg
    12001 ggctttgccg cctggcctac cgcccgtgat cgagggaccg acccccgccc ggctgcggcc
    12061 gccatcagac gtcgcattgg cgtcgctgca ggcatgcgat cagctcattc aaacacggca
    12121 tcgatcgcgt ggtccagcga ctcgcggatg tcggtgtcgt cggtgatcgg tcgcaccatg
    12181 gccatgcggc aggccgcgcg tgcatcgatg ccctgccgga tcagcgtggc ggcatagacc
    12241 accaggcgcg tcgaaatgcc ctcgtcgaga ccatggccct tgagtcgacg cgccgcacca
    12301 gccacctgca ccagcgcggc agcgacggct gcgtcgatcc cggtttcggc aaccacgatg
    12361 cgcgcctcca gcgcagcctc gggatagtcg aagtccaggg cggcaaagcg ctgccgcgtc
    12421 gaggtcttga ggtccttcat caggctctgg tagcccgggt tgtacgacac gacgagctgg
    12481 aagtcggcgt gcgcgcgcac cagttcgccc ttcttgtcga gcggcagcgc acgtcggtgg
    12541 tcggtgaggg cgtggatgac gaccgtggtg tcctggcgcg cctccaccac ctcgtccagg
    12601 tagcagatgg caccgtgacg cgcggccgtc gtcagcggcc catcctgcca gcgcgtgccg
    12661 tcgggttcga gcaggaagcg ccccacgagg tcggccgcgg tcatgtcctc gttgcacgcc
    12721 accgtgacca gggggcgttg cagcttccag gccatgtgct cgacgaaacg gctcttgccg
    12781 cagccggtgg gacccttgag catgaccggc aggcgcgccg cataggcggc ctcgaacagc
    12841 tcgatctcac cgccctgcgg ggcatagaag ggctcggcgg cgacgcgcca gtcagcgagt
    12901 gggttcatga cacggcggga cgttggccag gttggacggc gcctgccggt cgatcaccga
    12961 gcgcgatggt cggcacgcgc cgcacaccga cggacctgct ggggtgacag gcacgggcgc
    13021 cgccgtgggt tcagcggtgc acgcccgcca gcttgtcgcg ccagccggga tacagcttgt
    13081 cggcgtcgcc ggggaaggag tcgaacgcgc gcgcgaactc gcggtgctcg cgcgcccatt
    13141 cgatcgggtc ggccttggcc ttccagcatt cgtaggcctg gcgcagactt cgggcgccat
    13201 cggccggcga gtcgatgtga ccatagctgc cgccgccggc ggtgttgatg acgttgccgt
    13261 ggcccaggtt ttcgaagaag ccaggcaggc gcagcgcgtt catgccgccg ctgatgatgg
    13321 gcgtggttgg cctcatgccc agccactcct ggtgatacgc ggggccggtg tagctgtcgc
    13381 gctcgatgat gtaggcaatg gcgcggtcgt ccttctcgcc ttccatcttg ccgtagccca
    13441 tggtgccgac gtggatgccg gaggccccct gcaaccggct catcttggcc agcacgtagg
    13501 cggtgtagcc gcgcttgctc tgcgggctgg tcaccgcccc gtggccggcg cggtggtagt
    13561 gcaggtactg gttggggaac cagcggcgcg cggtggtgac catgccgggc ccgcccacgt
    13621 agccgtcgac caggaacgcc accttgtcgg cgtccgggcc gaaggtttcg aggatgtact
    13681 cgccgcgcgc gagcatttcg ctgtggtcgt cggcggtgat gttggccgag aagatcttcg
    13741 cttcaccggt cctgtccatg gcccgcttca tcgcgtcgcg aaccagcggc atcacctgct
    13801 tcatcggcgc gaagacctgg ttgccctgcg gttcgtcgtt cttgatgaag tcgccgccga
    13861 gccagaactc gtaggccgcc tgggcaaacg gttcgggccg caggccaagc ttgggcttga
    13921 tgatcgtgcc ggcgatgtag ccgccatcct tcagcgggcg gccaagcacg cgccacaggt
    13981 cgctgatgtc cttgctcgga ccgtcgaaca actgcagggc acgcggcggc acccagaagt
    14041 cgtacatctt ggcgtactcg atgtcgccca tgccctggtt gttgccgatc gccagcgtca
    14101 gaaacgagac gatcatcatc cggccatcga tgaggttgcg atcgaacagg tcgagggggt
    14161 aggcgatgcg catttcctcg ctgacctcgt cgatcaggta gacgagcgca tcgacccctt
    14221 tggtgaactc gtcggtggtc gagacctcga cgttggtgcc ggtcgatgac tcggccgcga
    14281 agtgcgccgc cgccgccagg tagccgtgcc cggccttcgg ccgcatcttg taggccacca
    14341 ggatgtgttt gccgccgcgg atcaggtctt cttctcgcag gctcaggtcg gcatagcggt
    14401 tggattggtc catctggggc tcctggggtg ggttcgctgg gatgcgaggc actttagaag
    14461 accgattgaa taagtacaac taaaataatt tggtgcttga attaagtatt tattgaattc
    14521 accccgccca cctggccgaa cttgaacacg gccgccgggc gcgaccgccc gtgccgtgag
    14581 cggtgcgcct ggtacgacgt gccgccgtgt cagaagtcga ccagactgag gccgatcgaa
    14641 aacaccgtgc gtttgaagtt gtagtccagc aggctgtcgc catagccgtg aaacagcgcc
    14701 gtgtacaggc gcaggctgga aaagctgttg ccccagccgt cgcccagcga gcgcgtccat
    14761 tccagccggc ccgagccgta gcggttgaag ctgccggtgt aggtggcgcg cagcgtgttc
    14821 ttggcgtcca cgttccaggc caggctgagg tcgccgcgcc cgatgtggcg cacgatgtcg
    14881 gggttgttgt ccttggcgct gtcttccggc aggcgcttcc acagccgcag ttgcaggttc
    14941 agcgcgttgc cgcgctcgaa gcccgccatc aggtaggcgc ggttccagct gcgcgacagc
    15001 gggtcgctct ggccgttgga ctggtgcgcc aggccgatgc cgctgtagcg ccacagccag
    15061 ccgccgggca gcttgagcgt ggtcgggtag acgtagatca gctcgggcat gtggtcggtg
    15121 ttgcgaaacg gccgcgacag gctgccgttg aacacctgcc agtacgactg ctgggtgtag
    15181 gcggcccaca gcgaatcgcg caacgtgccg ccggcggggg tgagcagccc gccagccagc
    15241 ttggtgcgca ccgacagtgc cagccgcatt tcgcgcttgc cgtagtcgat cggcgtgatg
    15301 gcgctgttgg ccgggttggg cgaggtcggc aggcggttga tgtggtcgcc ggcggcgatc
    15361 gacgccgtcg tcggatgaaa ggcgcgcagg ccgaaggtcg ggcagctgga gcccgactcc
    15421 agttcccaga agcgcgacag gtcggaaaac tggcggtcgc ggcagccctg ctccaacccg
    15481 acgccgacga tgccggccga gcccgacacc agggccccgt cggcgcgctc gggctgcgtc
    15541 gtggccagcg cctgggccag ttcgccacgc accgccgccg cctgcgcact gggtgccgca
    15601 ttgcgggtgg gcgtggcctc gaccgactgc gccttctgtg cgatggcttc gatcagctgc
    15661 cgctgtccgt gcgcccagcc gtcgaaacag gccaggcgct gcgcgtcggg ctggcccaga
    15721 caggcctgcc aggcggtggc ggcgtcgatg cgttcggcgc cggccggcat ggcgggcgat
    15781 tgcgcgctgg ccatggcggg cagcgccagg gccagacaga accacagggg gtgggggccg
    15841 gaagggtgca tgtgaaacca gaacgatggg cagcgcaaaa agaccgacgg gccagcaccg
    15901 gcccgcgggc accgattgtg cgtgccgcgg cggcgcgcct gcatccagtt gtgtccacgg
    15961 ccccggcggc gcaggtcggc cgcggtttat gaacccaatc cggctccgac gcccgaacca
    16021 tctgcgccaa aagctattgt tttcatagta catgggcatg cggaccgacg ctgcagctac
    16081 cgccagcggc tggttacagt gacgctccat gacgccttct ccactgctgt ttccgctgca
    16141 gggcgcgctg cggcgcgtgc tgtacgtcgg cctgtacgag gcgctggcga tcgcggtcgt
    16201 caccgtggcc ttcgtcgctg gcgaccgcag cctgggttcg gccagcggcg tggcggtggg
    16261 cagctcgctg atcgcggtgc tgtggaacct ggtgttcaac gccgccttcg aacgctggga
    16321 gtcgcgccag cgcgtgcgcg ggcgcagccg ggcgcggcgg ctggcgcatg ccatcggctt
    16381 cgagggcggg ctggcgctgt ggctggtgcc cttcatggcc tggtggctgg aggtgggcct
    16441 gtggcaggcg ctgctgctgg acctggggtt gctgctgttc tttctggcct acaccttcgt
    16501 cttcacctgg gtgttcgacc gcctgtttgg acttccgcgc gccgccgcgt gacaatgccg
    16561 caccatgctt gaacttgccg acatccagca ggccgccgag cgcgtgcgcg gtcaggtgct
    16621 ggacaccccc tgcgtcgaat cgcgcacgct gtcgcagctc accggcacgc agttgttcct
    16681 gaagatggaa aacctgcagt tcaccgcctc gttcaaggag cgcggggcct gcaacaagct
    16741 ggcgcagctg acggaccagg aaaaggcacg cggcgtgatc gccatgagcg ccggcaacca
    16801 cgcccagggg gtggcctacc acgccgagcg gctgggcatt ccggccacca tcgtcatgcc
    16861 cagcttcacc cccggggtga aggtggcgcg cacgcgcggc ttcggcgccc gggtgatcct
    16921 gcacggcgac tcgctggaag cggcctacca gcacgccaga accctggccg aagcgcagaa
    16981 cctggtgttc gtccacccct acgacgacct ggccatcatg gccgggcagg gcacgctggc
    17041 gctggaaatg ctggccgccc agccggagct ggacaccctg gtggtgccgg tgggcggcgg
    17101 cggcctgatc tccggcatcg ccaccgcggc caaggcgctg cggcccggca tccgggtgat
    17161 cggcgtgcag acccagcgct atccggccat ggtcaacgcg gtgcagggca cgcgccatgc
    17221 gcagggcggc aacaccatcg ccgaaggcat tgccgtgggc gcgccgggcg cgcactgcct
    17281 ggaggtgatt cgccagcgcg tcgacgacct gctgctggtg gacgaaggcg acatcgagca
    17341 agccatcgtg ctgctgctgg agatcgaaaa gaccctggcc gaaggtgcgg gcgcggcggc
    17401 gctggcggcc ctgctgcgcc acggcgaacg ctttgccggc cgcaaggtcg gcatggtgct
    17461 gtcgggcggc aacatcgacc cgcagctgct ggcgcagatc atcggccgcg ggctggtgcg
    17521 ttcggggcgg ctgatccgca tcgtcatcag cgcgcgcgac gtgcccggct cgctggcgcg
    17581 catcaccgcc ctggtggccg aagccggcgc caacgtcgac gaggtgcacc accagcgcgc
    17641 cttcaccctg ctggcggcgc agaacgtgga gatcgagatg gtgctgcaga cgcgcggcca
    17701 cgagcatgtg caggagctgc tgcagcgcct gcgcggcgcc gggttggagg tgcggctggc
    17761 caccgcctga cccggcccgg ggcggcggcc ggccgatctg cgctatgctc gctcgccttg
    17821 cggtcgccgg cccgttggca gggcggcgcc cgcacctgtc tgccgcccca tgccaccgcc
    17881 gccctccgca ccccaggccc cgcaccgcca cccgccgctg ctgctgcgtt cggccggctg
    17941 gcgcctgggc gtggccgcgg cgctgggcgc actgctgctg ctgctgtggc gctgggcgct
    18001 ggcgggcgcc tcgcactgac ggatccgagc tgaattgtct gcgccgatcc tcctgcacga
    18061 cctgacgctg ggctatgacg gccacccggc ggtgcatcac gtgtcgggcg tgatcgaggc
    18121 cggcgccctg ctggccgtgg tggggcccaa cggcgccggc aagtccaccc tcatcaaggg
    18181 cctggccggg ctgctcaagc cgctgggcgg gcgcatcgag gggctggccg gccagcgcgt
    18241 ggcctacctg ccgcagcagg ccacgctgga gcgcggcttt cccgtcacgg tgggcgaatt
    18301 cgcggccatg gggctgtggc gggaatgcgg ggcgttcggc ggcttctcgc ccgcccagcg
    18361 ccagcgcgtg cgcgaggcgc tggcgcaggt ggggctggaa ggtcacgcac gccagcccat
    18421 cgacaccctg tcgggcgggc agttgcagcg cacgctgttt gcacgcctga tgctgcagga
    18481 cgccgacgtg ctgctgctgg acgagccctt tgccgccatc gaccagcgca ccaccgacga
    18541 gctgctggcg ctgctgcgcc agtggcacgc cggcggcaag acggtgctgg cggtgctgca
    18601 cgatctgcac caggtgcgcg ccagcttccc gcgcacgctg atgctggcgc gcgagctgcg
    18661 ggcctggggc gacaccgcgg acgtgctgac accggccaac ctgcagggcc tgcgcgtgat
    18721 gcccgaggcc ttcgacgagc acgcgccggt gtgcgaggcc gcccccggcc acgaccacag
    18781 ccaccatggc cgcgcccacc atcacgcgga gcacgaccgg tgagcggcga gctgctggag
    18841 ggcggcctgt gggcgctgct ggtggcaccg ctgaccgagt tcggcttcat gcgccgggcg
    18901 ctggtgggct gcctggcgct gtcgctgggc tgcgcgccgg tgggcgtgtt cctgctgctg
    18961 cggcgcatga gcctgaccgg cgacgccatg gcgcacgcca tcctgccggg cgcggccatc
    19021 ggctacctgc tgtcgggcct gtcgctgacg gcgatgtcgc tgggcggggt ggcggccggc
    19081 ctgctggtgg ccgtgggctc ggggctggtg gcgcgctcca ccgtgctgcg cgaggacgcc
    19141 agcctggccg ccttctacct gctgtcgctg gcgctggggg tgctgatcgt gtccacgcgc
    19201 ggctccagcg tcgatctgct gcacgtgctg ttcggcagcg tgctggcgct ggacgacgcc
    19261 gcgctcgggc tgctggtggg catcgccctg ctgacgctgg ccacgctggc gctgctgtac
    19321 cggccgctgg tgctggaatg tctggatgcg cagtttctgc gcagcgtcag ccgcttttcg
    19381 cccgtcgccc actacggctt tctggtgctg gtggtgatca acctggtggc cggctttcac
    19441 gccctgggca cgctgatggc ggtgggcatc atggtgctgc cggcagccac cgcgcgcctg
    19501 tgggtgcgcg gcgtgccggc gctgatggcg ctggccgcag ggctggcgct ggccgcctgc
    19561 gtggtcgggc tgctgctgtc ctaccacctg gactggccga ccagcccgac catcgtgctg
    19621 tgcctgggcg gggtgtacct gctgtcgctg gtggcgggac gcttcggcgc ctggcgccag
    19681 ggccagcatg ggcggcagcg ccatctgcgc ggctgagctg gccgggccgc atcgaaatgc
    19741 atcatcaaat cggcatccgg cgcacatcag gaaagcgcaa acagctatgc aattgatagt
    19801 acaccgacgg atgcgcaggc accttgtgcg cgccaccctg gccctgctga cggcgctgcc
    19861 cgcgctggcc cagggcgcgg cgccactgcg cgtggtggcc agcttcagtc tgctggccga
    19921 catggtgcgg caggtcggcg gcgaccgcgt gacggtgagc gcgctggtcg gccccggcgc
    19981 cgacgcccat gtgttccagc ccacgccggc ccaggcgagc caggtcggtc aggcgcagct
    20041 ggtggtgacc aacggcctgg gctacgaagg ctggctgccg cgcctgctgc gcagcgccgg
    20101 ctaccgcggc ccgcaggtgg tggccgcccg cggcatcgag ccgctgccgg ccacgcccgg
    20161 ccacggtcac ggcggtcacg gccgcgacgc gcatggccac accgacccgc acgcctggca
    20221 aagcgtgccg cacgccatgg tctacgtgaa gaacatcgcc gacggcctgt gcgcggccga
    20281 cccggccggc tgcgccgact accgtgccgg cgcggcgcgc tacagcgccg agctgaaggc
    20341 gctggacgcc gagatccgcg ccgcctgggc ccccatcccg gcaccgcgcc gcctggtcat
    20401 caccgcacac gccgcctacg cctactacgg cgcggcctat ggcgtgcgct ttctggcgcc
    20461 gcagggcgtg agcaccgaca gcgaggcctc ggcccggggt gtggcgcagc tggtgcggca
    20521 gatccgccag cagggcatac gggcgctgtt catcgaaaac atcgccgacc cgcgactgat
    20581 cgagcagatc gcccgtgaaa ccggcgtgcg tcccgccggc cggctgtacg ccgacgcctt
    20641 gaccgaagcc gacggcccgg cgcccagcta cgtcgccctg atgcgcgcca atacccgcgc
    20701 cctgacccag gccgtgggcg gcccgtaaaa tcggccccct cgccgccaag gagcgttgca
    20761 gcgggcaccg cgccgggcgc ggccggcccg tcaggcttgg tggcggttgc ccgtctgaac
    20821 cgcaacgacg ctcacctcga ccgacccgat ccaggtgagt gccatgtccg ctgccgcccc
    20881 gccccccccg ccgccgatgc gtctgtccgg cctggagccg ctgaccctcg gcgaaggcac
    20941 gctgttcgtc aacgtcggcg agcgcaccaa cgtcaccggc tc
//
LOCUS       DAOOOG010000025        20824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_2517_length_20824_cov_31.632577, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000025 DAOOOG010000000
VERSION     DAOOOG010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 20824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20824
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2517_length_20824_cov_31.632577"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PK242_03545"
     CDS             complement(<1..643)
                     /locus_tag="PK242_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOZ93122.1"
                     /translation="MNAPLHHELPTPLLDAAQARARIDAQWDGDILRQLTDYIAIPAK
                     SPVFDPDWAAHGFIDAVVRQAAGWVEAQKVPGLTLEVVRLPGRTPVIFFEIPATRPGS
                     TQTALLYGHLDKQPEFTGWRADLGPWTPKLEDGKLYGRGGADDGYAVYAAIAAVQALK
                     AQQLPHSRLVGLIETSEESGSPDLLPYIDALRPRLGDVGLVVCLDSGAGNYDQF"
     gene            728..1429
                     /locus_tag="PK242_03550"
     CDS             728..1429
                     /locus_tag="PK242_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOZ93123.1"
                     /translation="MNTLNDFPITRKWPARHPERLQLYSLPTPNGVKVSIALEELGLP
                     YEAHRVSFDTQEQLSPEFLSLNPNNKIPAILDPHGPGGRPLALWESGAILLYLADKTG
                     QLLSQDPATRWHTVQWLMWQMGGAGPMFGQLGFFHKFAGKDWDDKRPRDRYVAESARL
                     LGVLDRQLQGRDWVLGSDYSIADIAIFPWVRNLVGFYEAGELVGFERFTQVRRVLDAF
                     VARPAVQRGLQVPAG"
     gene            complement(1504..1860)
                     /locus_tag="PK242_03555"
     CDS             complement(1504..1860)
                     /locus_tag="PK242_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOZ93124.1"
                     /translation="MSEPTQREWRFYIDDMIDFAGRVLAYTRDVDEGAFVANGLIYDA
                     TLRNLELIGEAATHVPDEVRHAHAEIPWRMLVATRNRLIHGYLGLDDDTLWSIVHDEV
                     PALLRQLEALKAASAR"
     gene            complement(1853..2143)
                     /locus_tag="PK242_03560"
     CDS             complement(1853..2143)
                     /locus_tag="PK242_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOZ93125.1"
                     /translation="MNRRPVLELLRRSKPELQARFGVTRLALFGSAARDGMHAGSDVD
                     VLVAFDGPATAPRYFGVQFYLEDLLGMPVDLVTEKALRPRLRPFVEKDLARV"
     gene            2218..2574
                     /locus_tag="PK242_03565"
                     /pseudo
     CDS             2218..2574
                     /locus_tag="PK242_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
     gene            2657..3082
                     /locus_tag="PK242_03570"
     CDS             2657..3082
                     /locus_tag="PK242_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93126.1"
                     /translation="MQATLKPEPEAGHDPCTQLLVRSWRLIMVRGVECPLVTHEFRCA
                     CGLDGREVLRAFCAFLCALAFSQRRRLWVNPPGEPRLTADETDMLRLIAAAQNGCAPL
                     LDAQLSGLARQPLRAALEHSVRELGALLQARQLRLPRPA"
     gene            3291..4838
                     /locus_tag="PK242_03575"
     CDS             3291..4838
                     /locus_tag="PK242_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HOZ93127.1"
                     /translation="MSPLLRLLSLGAAGAALLAGCALPPLPERTASRALDEGEALQTS
                     LGRALAPQVSRHPGRSGIHLLSDPLDAFAARMLLAHQAERSLDVQYYIWRGDHTGTLL
                     LHELVRAAERGVRVRLLLDDGGTGGLDRELAALAQHPRIEVRLFNPLLPRWLKPLGYL
                     TDFARVNRRMHNKSFTADNQASIVGGRNVGDEYFGATEGVVFADLDVLAVGPIVSEVS
                     ADFDRYWASASSWPAQDVLPAADPSTVPALAARAGALAQSDAGRAYAQAAQRTPFMTD
                     LLNQRLALQWSRVDMVSDDPAKGLAQAQRSGLLVDQLHRALGVPQRSVALVSPYFVPT
                     NAGAEALIRLQQRGVKVLILTNAYEASDVPLVHAGYARHRTALLQAGVALYEMQRLAP
                     AARGSLVRPNRLGSSGSSLHAKVFAVDGQRAFVGSFNFDPRSALLNTELGFVIHSPEL
                     ARQIEQTFDTEVPRSAYTLRLSESGQIEWLSAAEPPVLQRDEPGSTWWSRIMLGLLSL
                     LPIDGLL"
     gene            complement(4954..5718)
                     /locus_tag="PK242_03580"
     CDS             complement(4954..5718)
                     /locus_tag="PK242_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ93128.1"
                     /translation="MPNTHLLIGQGAHKVIALHGWFGHARGWGPLTQHLNGSDFSYAF
                     MDQRGYGGMRGSGGPYTVEQIAQDALALADQLGWQRFSLIGHSMGGSAIQQVLVAAPG
                     RVRALVAITPVSAAGVPFDEQGWQLFTAAGSDPAARRGIIDFTTGNRLTGTWLDAMVA
                     STQQHSDDAAVAGYLLSWARTAFVEKIQGQQLPVLVLPGEHDPALGEAACRATWMQHY
                     PNAQLELVRNAGHYPMDEAPIILATQIEKFLATVPA"
     gene            complement(5828..6412)
                     /locus_tag="PK242_03585"
     CDS             complement(5828..6412)
                     /locus_tag="PK242_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93129.1"
                     /translation="MPPPSAPDRRRACQRLLGAALLSPLLPALLACAGGASGGWLLPV
                     ARLDELLARRFPVTRSLAGLADLTLHSPRLSLLPAANRVATALDLVLTERLGGRRYTG
                     AIELDSALQLDLQQGAVRLSAVRVQRLGLDQLPPAAQTLLARHAPPVVEQLLEGLVIY
                     RLPPERLATLRAAGRGAPPQLRVQPDGLRLELAP"
     gene            complement(6537..7877)
                     /locus_tag="PK242_03590"
     CDS             complement(6537..7877)
                     /locus_tag="PK242_03590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOZ93130.1"
                     /translation="MKVTVFGTGYVGLVQGAVLADVGHHVLCIDVDEAKVAALRAGRI
                     PIHEPGLDALVRAGQAAGRLRFSTDAAEGVAHGELLFLAVGTPPDEDGSADLQHVLAV
                     ARSIATHMTVPKTLVNKSTVPVGTADRVAEVVRAVLRERGVQIAFDVVSNPEFLKEGA
                     AVADCQRPDRIVIGTASAAAEAQLRELYAPFNRNHDRMVVMDVKSAELTKYAANAMLA
                     TKISFINEIASLAERLGADVEAVRRGIGSDPRIGYHFIYPGMGYGGSCFPKDVQALIH
                     TAQSVGFEPELLQAVERRNQAQRQRLGQRIVAHYGGTLAGKTIAVWGLAFKPDTDDMR
                     EAPSRTLLAQLWAAGARVRAHDPVAMDEARRIFGARPDLVLCDSPSEALTGADALAIV
                     TEWKQFRVPDFDQLAQRLADRIVFDGRNLYEPALLARHGLAYESIGRPGVRPLT"
     gene            complement(7888..9414)
                     /locus_tag="PK242_03595"
     CDS             complement(7888..9414)
                     /locus_tag="PK242_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOZ93131.1"
                     /translation="MNLTAAPGVPLVRSPVTHPLAWLLALALAHVAVRVAISPALKWD
                     EAEQMLWSQQLALGYGAQPPLYTWLQWGVNRLVGPSVLALSLLKHALLALTYALMWLA
                     GRELLAPRGAWWAAASLMLLPTLGWYSVRDHTHTVLVTAMACGAWWLLLRIVRQPRPL
                     EFAALGLVCGLGMLAKYSFALVAGAMLLAALSEPQARRALLSRGWWWAPLVGLLVVLP
                     HLGWLLSHLSEATAGTLSKMEIDAARGPLKGARHLLQGVAGTLLLFALFALWAFGGAW
                     WRSPRQPALPWAQRVFGRYLLLVLVALLGMVLAGVTEFKGRWLLPLLCVVPLAAFAAR
                     PELQQHPRGARYTGAIVAMALLILLAAGLRPWFSGLRGEVDELNHPAAELGAALSAAG
                     YDGRSPIIAADHMMAGLLRTRFPRASVGSCTVKDDDPLSDCVAALVARARAAGQGWLL
                     VSRTDRLEPDWWALATARLPARPVRSIELPLRMVRDGTPPAHYQYLWQPAAPAPDQPQ
                     "
     gene            9497..10528
                     /locus_tag="PK242_03600"
     CDS             9497..10528
                     /locus_tag="PK242_03600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ93132.1"
                     /translation="MSTDRSTAPDVSIVVPIYNEVDNLPDLVERIAAAMAGQALSFEL
                     LAVDDGSSDGSRARLRELAAGRPWLRPVLLARNYGQSSALQAGFDRVRGRYVVTLDAD
                     LQNEPGDIPLLLERLENDPEVDMVSGWRKDRQDAELSRKLPSRIANRLISRATGVHLH
                     DYGCALKAYRRPIIDRIRLYGELHRFIPSLAREAGARITEVPVRHHARTRGVSKYGID
                     RTFRVILDLILIVFFMRYRQRPLHAFGGLGLWLAAPGGLILVWLLLQKLLGEDIGGRP
                     LLLVGVMLVLMGAQLIAAGLIGELLIRVYHEAGGAPQFHATELRPDDEPQQASAPVPP
                     APTATNKIA"
     gene            10525..11454
                     /locus_tag="PK242_03605"
     CDS             10525..11454
                     /locus_tag="PK242_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOZ93133.1"
                     /translation="MKRPLKAVLRALLGLALLAGVLTLADPARVLAQWRQADPAWLLA
                     GLLAAIGSNAVSALRWRALARWLGAELSAREAARWYFQAIGLNTLLPGAVVGGDLYRA
                     VMLRRAGQATAAAGWSVLLDRLSGLWMLCAIGALGAAACAPVLGPWLHLPPAPLAALL
                     LAGSALWLALPWALPALGRARPGSGGWLAPLRLAAARPDFRRQLLWQALASAAVQGLS
                     AAALAAGALALGVVQPLAVWAWAIAPVFLMAALPVSVGGWGTREAAAVAALAPFGVAP
                     AAAVGVGVIYGLYGLAQGALGALAFGLPRVATE"
     gene            complement(11633..12760)
                     /gene="hppD"
                     /locus_tag="PK242_03610"
     CDS             complement(11633..12760)
                     /gene="hppD"
                     /locus_tag="PK242_03610"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HOZ93134.1"
                     /translation="MPPASPAATTWDNPMGTDGFEFIEYAAPDPQAMGALFERMGFAA
                     VARHRHKNVTLYRQGEINFIINAEPDSFAQRFARLHGPSICAIAFRVQDAKAAYERAI
                     SLGAWGYAGQAGPGELNIPAIKGIGDSIIYLIDRWRGKNGAREGDLGNIGFYDVDFYP
                     LPGVQGGAAVNPEGYGMTVIDHLTHNVHKGRMDEWASFYERLFNFREVRYFDIEGQAT
                     GVKSKAMTSPCGKIRIPINEEGNEQKGQIQEYLDLYKGEGVQHIALGCKDIYHAVDQL
                     GLAGVKLLNTSDTYYELLPGRIPDLKEPIAELQARNILVDGHDGELLLQIFSENQLGP
                     IFFEFIQRKGNEGFGEGNFKALFETMELDQMRRGVLTQKPA"
     gene            12962..13438
                     /locus_tag="PK242_03615"
     CDS             12962..13438
                     /locus_tag="PK242_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOZ93135.1"
                     /translation="MTAALDRLDRAILARLQTDGRETHEAIGQQVGLSASAVLRRIRR
                     LEEAGVIDRYVALLRPESLGLGLSAYVNVRLEKRAEHAKRNPMDEFRASVQGWPEVVE
                     CVALTGEMDFLLHLVVQDMGAYSRFMMDTLLRHPSVQDCKTSFVMDRVKTTTALPI"
     gene            13627..14322
                     /locus_tag="PK242_03620"
     CDS             13627..14322
                     /locus_tag="PK242_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ93136.1"
                     /translation="MNQLHPPVIDTPIDPAPRRPSRRSAVVPIREVGPEHRERIARHL
                     LALDEHDRYLRFGYAAGDAQIRQYVDGLNFERDHVFGIFNRRLTLIAMAHLAYPNDFA
                     SAGFAEFGVSVAKHARGRGYGKRLFERAAIHAVNDGVQTLYIHALSENTAMLRIARQA
                     GAVVEHAGSESQAHLSLPPATFASRVSELLADQVGQVDFGLKTAASQARQLLATVQEV
                     RAAVRDGQHRSDV"
     gene            14459..15349
                     /locus_tag="PK242_03625"
     CDS             14459..15349
                     /locus_tag="PK242_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOZ93137.1"
                     /translation="MADPPSARNPERSNEREDKRSLFQKLIEFIRPGPDSTHELIETL
                     ADAEDKEVINAESRVMLEGVLRLSDMTAGDAMVAAPRMDLLDIDAPYEQLLRDVITTA
                     HSRFPVYQGERENIIGILLAKDLLKLQRAPELNLRTLLRPAMFIPESKGLSDLLRAFR
                     ISRNHLAMVVDEFGRTAGLITIEDVLEQIVGEIEDEFDIDADEGDIFALADHSWRVAG
                     DTPLERVNEAFGVRLGADPQGDLEAEFDTVGGLIAHEMGHVPKRGEHHRMGTLDFHVL
                     HTRGGAVRWFKVVRVPPTEA"
     gene            15349..16944
                     /gene="lnt"
                     /locus_tag="PK242_03630"
     CDS             15349..16944
                     /gene="lnt"
                     /locus_tag="PK242_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOZ93138.1"
                     /translation="MRRVHALASPVAALLAGVLQALSIADPWQGAPRWWLQTFSLAWL
                     VWQLVHQAERQSSWSASTWERPQTAPAWRRALALAGLFALGWLAGSFWWLFTSMHTYG
                     GLHPLLAAAAVLALAGFLALYYVVAGAAFVALAPTRRPVRAALLFAALWTLAELARGR
                     WLTGFPWGAGGYAQVDGPLAFLARYVGVYGVGFVGAALAALLALAPQVRWRQPRTLAG
                     TAALLALAGSLWGGRQMALDTPGAPALTSAVELLQGNIPQDQKFIPGTGMADALRWYG
                     AQLQASTAPLVVAPETAIPLLPRQLPEGYWEALRARYAQGPQAALLGVPLGDLEHGYT
                     NSVVGLAPGQAAPYRYDKHHLVPFGEFIPPLFRWFTELMRIPLGDFSRGAVGQLSFVW
                     QGQRLAPNICYEDLFGEELAARFIDPATAPTAFVNLSNIAWFGDGVAIDQHLAIGRMR
                     ALEFERPMLRATNTGATAIIDHRGQVTHRLARATRGVLRGQFEGRSHLTPYAAWAARL
                     GLWPLWLGCAALVVAALARQRRG"
     gene            17000..17512
                     /gene="fabA"
                     /locus_tag="PK242_03635"
     CDS             17000..17512
                     /gene="fabA"
                     /locus_tag="PK242_03635"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HOZ93139.1"
                     /translation="MADSFSYDQLIASGEGRLFGPEAGRLPLPPMLMFDRITHIDDDG
                     GAHGLGRIEAELDVKPGLWFFACHFQGDPVMPGCLGLDAMWQLIGFYLTWLRLPGRGR
                     ALGAGEVKFTGEVGPDVKLVRYEIDIKRVIKRKLNMAIGDARLLADGKEIYVANDLRV
                     GLFTREGQAS"
     gene            17509..18729
                     /gene="fabB"
                     /locus_tag="PK242_03640"
     CDS             17509..18729
                     /gene="fabB"
                     /locus_tag="PK242_03640"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HOZ93140.1"
                     /translation="MSTQRRVVITGAGIVSCIGNDLDTVEASLRAGRSGIRAVPRFTE
                     MGLRSQVAGVPEIDLEARIDRKELRFMGDAAAYAQIALADAIAQAGLTPAQVSHPRTG
                     LIMGSGGGSPANQIEAADTLREKGIRRVGPYQVTRCMSSTVSACLATNFKVKGINYSI
                     TSACSTSAHCIGAAAQQIAWGMQDVMFAGGGEELSWGMAMLFDGMGAMSSKYNATPDK
                     ASRAYDAHRDGFVIAGGGGAVVLESLEHAQARGATILAEVVGFGATSDGEDMVAPSGD
                     GAIACMRQAIDGLDAPIDYINTHGTSTPVGDMQEVRAMRAVFGDAVPPFSSTKSLTGH
                     SLGATGVQEAIYCLIMLQKGFIAGSVNVETPDPELGAMPLVTATRDARLSTVLSNSFG
                     FGGTNASLVLRRWP"
     gene            18836..19756
                     /gene="glyQ"
                     /locus_tag="PK242_03645"
     CDS             18836..19756
                     /gene="glyQ"
                     /locus_tag="PK242_03645"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOZ93141.1"
                     /translation="MLTFQQIILSLQQYWADQGCALLQPYDMEVGAGTSHTATFLRAI
                     GPEPWRAAYVQPSRRPKDGRYGDNPNRLQHYYQYQVVLKPAPANILELYLGSLQALGF
                     DLKKNDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCRPATGEITY
                     GLERLAMYLQGVDNVYNLVWTEGPDGSKLTYGDVYHQNEVEQSTYNFEHSDADFLFTA
                     FAAHERQALHLIGQQLALPAYEQVLKAAHTFNLLDARGAISVTERAAYIGRIRTLARA
                     VAQSYYDSRQRLGFPLAPRAWVEQIEKPAA"
     gene            19768..>20824
                     /gene="glyS"
                     /locus_tag="PK242_03650"
     CDS             19768..>20824
                     /gene="glyS"
                     /locus_tag="PK242_03650"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOZ93142.1"
                     /translation="MTTQNLLVELFVEELPPKALRQLGEAFATTLAAQLQAQGLTAAD
                     SRVTPYASPRRLAAHITHVAAKAADRAVQLKLMPVSVGLDAQGQATPALLKKLQALGA
                     EPTVVPQLKRQPDGKAEALFLDSVVTGATLDSGLQKALDEAIARLPIPKVMTYQLQAG
                     CARPGWDSVQFVRPAHGLVALHGETVVPVTALGLRAGRATHGHRFEAASDPVELASAD
                     DYALDLRQQGAVIASFEERRATLEAQIKAAATQAGPALTAIKDEALLDEVTALVERPN
                     VLVCAFEPEFLQVPQECLILTMKANQKYFPLLDAQGRLTHRFLVVSNITPDDPSAVIA
                     GNERVVRPRLADAKFFFD"
BASE COUNT         3021 a         7316 c         7399 g         3088 t
ORIGIN      
        1 agaactggtc gtagttgccg gcgcccgagt ccaggcacac caccaggccg acgtcgccca
       61 ggcgcgggcg cagggcgtcg atgtaaggca gcaggtcggg cgagccgctt tcttcgctgg
      121 tctcgatcag gccgaccagg cgcgagtgcg gcaactgctg cgccttcagc gcctgcacgg
      181 cggcgatggc ggcgtacacc gcatagccgt cgtcggcgcc gccgcggccg tacagcttgc
      241 cgtcttccag cttgggcgtc cacggcccca ggtcggcgcg ccagccggtg aattcgggct
      301 gcttgtcgag gtggccgtac agcagcgcgg tctgcgtgct gccgggtcgg gtggccggga
      361 tctcgaaaaa gatcaccggc gtgcgcccgg gcaggcgcac cacctccagt gtcaggccgg
      421 gcaccttctg cgcctcgacc cagccggcgg cctggcgcac cacggcgtcg atgaagccgt
      481 gcgccgccca gtccgggtcg aacaccggcg acttggccgg gatggcgatg tagtcggtga
      541 gctggcgcag gatgtcgccg tcccactggg cgtcgatgcg cgcgcgcgcc tgggcggcgt
      601 ccagcagggg ggtgggcagt tcgtggtgca ggggggcgtt catggcggca caagcgggtg
      661 gaagatgatc ggcgcacttt acccctgctg ccgccagtgc gccagccacc tgcgtatatt
      721 ggccgccatg aacaccctga acgacttccc catcacccgc aaatggccgg cgcgccaccc
      781 cgagcggctg cagctgtatt cgctgcccac gcccaacggc gtcaaggtgt cgatcgcgct
      841 ggaggagctg ggcctgccct acgaggcgca ccgcgtcagc ttcgacacgc aggagcagct
      901 gtcgcccgag ttcctgtcgc tcaacccgaa caacaagatc cccgccatcc tggacccgca
      961 cggccccggc gggcggccgc tggcgctgtg ggaatcgggc gccatcctgc tgtacctggc
     1021 cgacaagacc ggccagctgc tgtcgcagga ccccgccacg cgctggcaca ccgtgcagtg
     1081 gctgatgtgg caaatgggcg gcgccggccc catgttcggc cagctcggct tcttccacaa
     1141 gtttgccggc aaggattggg acgacaagcg cccgcgtgac cgctacgtgg ccgaaagcgc
     1201 gcgcctgctg ggcgtgctgg accgccagct gcagggccgc gactgggtgc tgggcagcga
     1261 ctacagcatc gccgatatcg ccatcttccc ctgggtgcgc aacctggtgg gcttttacga
     1321 agcgggcgag ctggtcggct tcgagcgctt cacccaggtg cgccgcgtgc tggacgcctt
     1381 cgtcgcccgg ccggcggtgc agcgcggcct gcaagtcccg gcggggtgac cttcggggtg
     1441 cggcggcggc gccgggacga tctggcactg cgtgagtgtg tgcccccccg cagcactgac
     1501 ggtctatctg gcgctggcgg ccttcagagc ctccaattgc cgcagcaagg cgggcacctc
     1561 gtcgtgcacg atgctccaga gcgtgtcatc gtcaaggccg agatacccgt gaatcagtcg
     1621 attgcgcgtc gccacgagca tccgccacgg aatctcggca tgcgcatgac gcacctcgtc
     1681 cgggacatgc gtggctgcct cgccaatcag ctcgagattg cgcaaggttg cgtcgtagat
     1741 caggccattg gcgacgaaag ccccctcgtc cacatcccgc gtataggcga gcacgcgccc
     1801 cgcgaagtcg atcatgtcgt cgatatagaa ccgccattca cgctgagtcg gctcagacac
     1861 gggccagatc cttctcgacg aacggacgca accgtggccg caacgccttc tcggtcacca
     1921 gatcaacggg catgcccagc aaatcctcca gatagaactg cacgccgaaa tagcgcggcg
     1981 ccgtggcggg gccatcaaag gcgaccagca cgtccacatc gctgccagcg tgcatcccat
     2041 cccgcgcggc agagccgaac aaggccaacc tggtcacgcc aaagcgcgcc tgcagttcgg
     2101 gcttgctgcg ccggagcagt tcgagtacag gtcgtcggtt catgtcgctg cttctgaagg
     2161 aggtttggct tcaaattctc gcaaatcgag gcggccgtgg ccacgcgctg gcacgccctg
     2221 cagtggctga tgtggcaaat gggcggcgcc ggccccatgt tcggccagct cggcttcttc
     2281 cacaagtttg ccggcaagga ttgggacgac aagcgcccgc gtgaccgcta cgtggccgaa
     2341 agcgcgcgcc tgctgggcgt gctggaccgc cagctgcagg gccgcgactg ggtgctgggc
     2401 agcgactaca gcatcgccga tatcgccatc ttcccctggg tgcgcaacct ggtgggtttt
     2461 tacgaggcgg gcgagctggt cggcttcgag cgctttgtcc aggtgcggcg cgtgctggac
     2521 gccttcgtcg cccggccggc ggtgcagcgg ggcctgcaag tcccggcggc ctgaggccac
     2581 ccgtcgcagg gcttgcaaat gagaattatt cgcatgtata gtcgtgcccc ataccaacga
     2641 ccgcgaggca cggcacatgc aggcaaccct gaaaccggaa cccgaggcgg gccacgaccc
     2701 ctgcacgcag ctgctggtgc gctcgtggcg gctgatcatg gtgcgcggcg tcgaatgccc
     2761 gctggtgacg cacgaattcc gctgcgcctg cggcctggac gggcgcgagg tgttgcgcgc
     2821 tttctgcgcc tttctgtgcg cgctggcgtt ttcgcagcgg cgccgcctgt gggtcaaccc
     2881 gccgggcgag ccgcggctga ccgccgatga aaccgacatg ctgaggctga tcgcggcggc
     2941 gcagaacggc tgcgcgccgc tgctcgacgc ccagttgagc gggctggcac gccagccgct
     3001 gcgcgccgcg ctggagcaca gcgtgcgcga gctgggcgcc ctgctgcagg cgcgccagct
     3061 gcgcctgccg cggccggcct gaggccgtcc aggcgcagcc agccagcggc gcccgacctc
     3121 ctggtgtggt gttgcgtgct tgagggaacc cccataagac cgctggactt ttggccggga
     3181 tgaggcgcag accacagcca tagctccgcc atggcgcgga tttgcaccca tggccgttga
     3241 ggatggcttg ggcgcacggg ttaccatgaa ccccctgctc gggcccaccg gtgtcacccc
     3301 ttctacgctt gctgtccctg ggcgcggccg gcgccgcctt gctggccggc tgcgccctgc
     3361 caccgcttcc cgagcggacg gcatcacgcg cgctcgatga gggcgaggcg ctgcagactt
     3421 cactgggccg cgccctggcc ccgcaggtct cgcggcaccc gggccgcagc ggcatccacc
     3481 tgctgtccga cccgctcgat gccttcgcgg cgcgcatgct gctggcccac caggccgagc
     3541 gcagcctcga cgtgcagtac tacatctggc gcggcgacca caccggcacc ctgctgctgc
     3601 acgagctggt ccgcgcggcc gaacgcgggg tgcgcgtgcg cctgctgctg gacgacggcg
     3661 gcaccggcgg cctcgaccgc gaactggcgg cgctggcgca gcatccccgc atcgaggtgc
     3721 ggctgttcaa tcctctcctg ccgcgctggc tcaagccgct gggctacctg accgacttcg
     3781 cccgcgtcaa ccggcgcatg cacaacaagt cgttcaccgc cgacaaccag gccagcatcg
     3841 tgggcgggcg caacgtgggc gatgaatact tcggtgccac cgagggcgtg gtgtttgccg
     3901 acctggatgt gctggcggtc ggccccatcg tgtccgaggt gtcggccgac ttcgaccgct
     3961 actgggccag cgcatcgtcc tggcccgccc aggacgtcct gccggcggcc gatccgagca
     4021 ccgtgcccgc gctggccgcc cgtgcaggcg cgctggccca gtccgacgcc ggccgcgcct
     4081 atgcccaggc ggcgcagcgc acgcccttca tgaccgatct gctgaaccag cggctggcgc
     4141 tgcaatggag ccgcgtggac atggtgagcg acgatccggc caagggcctg gcgcaggcgc
     4201 agcgcagcgg cctgctggtc gatcaactgc accgcgcgct gggcgtgccg cagcgctcgg
     4261 tggccctggt gtcaccgtat ttcgtgccca cgaacgcggg cgccgaggcc ttgatacgtc
     4321 tgcagcagcg cggcgtgaag gtgctcatcc tgaccaacgc ctacgaggcc agcgacgtgc
     4381 ccctggtgca cgccggctac gccaggcacc gcacggcgct gctgcaggcc ggcgtggcgc
     4441 tgtacgagat gcagcgcctc gcgccggcgg cccggggctc gctcgtgcgt ccgaatcggc
     4501 tgggcagctc gggctcgagc ctgcacgcca aggtcttcgc ggtcgacggc cagcgcgcct
     4561 tcgtcggctc gttcaacttc gacccgcgct cggcgctgct caacaccgag ctgggcttcg
     4621 tcatccacag ccccgagctg gcgcgccaga tcgaacagac tttcgacacc gaggtgccgc
     4681 gcagcgccta caccctgcgc ctgtccgaga gcgggcagat cgagtggctg agcgccgctg
     4741 agccgccggt gctccagcgc gacgaaccgg gcagcacctg gtggagccgc atcatgctgg
     4801 gcctgctgtc gctgctgccg atcgacgggc tgctgtaggg tcgccgcgcg ccatggcgcg
     4861 catcgccgga acccgcacga tccgagccaa atcggcatcc agcgcccatc cagtcagcgc
     4921 cggcagctat gtttttgata gtttgcgcgg cgactacgcc ggcaccgtgg cgaggaattt
     4981 ctcgatctgc gtggccagga tgatgggtgc ctcgtccatc gggtagtggc cggcgttgcg
     5041 caccagctcg agctgcgcgt tcgggtagtg ctgcatccag gtggcgcggc aggcggcttc
     5101 gcccagtgcg gggtcgtgtt caccgggcag caccagcacc ggcagctgct ggccctggat
     5161 cttctcgacg aaggcggtcc gggcccagga cagcaggtag ccggccaccg ccgcgtcgtc
     5221 cgaatgctgc tgcgtgctgg ccaccatggc gtccagccag gtgccggtga ggcggttgcc
     5281 ggtggtgaag tcgatgatgc cgcggcgcgc cgccgggtcg ctgccggcgg cggtgaacag
     5341 ttgccagccc tgctcgtcga acggcacgcc ggcggccgac acgggggtga tggccaccag
     5401 cgcgcgcacg cgcccgggcg cggccaccag cacctgctgg atggcgctgc cgcccatgga
     5461 atggccgatg agcgaaaagc gctgccagcc cagctggtcg gccagggcca gcgcgtcctg
     5521 cgcgatctgc tcgacggtgt agggcccgcc cgagccgcgc atgccgccgt agccgcgctg
     5581 gtccatgaag gcgtagctga agtcgctgcc gttcaggtgc tgggtcagcg ggccccagcc
     5641 gcgcgcgtgg ccgaaccagc cgtgcagcgc gatgaccttg tgcgcgccct ggccgatcag
     5701 caggtgggtg ttgggcatgg catgtctcct tgggtggtgg tggatatgag gtgctgcgat
     5761 catcatacga aggtcgcagg cacgaccggg cggtctccgc gggccggcgc gatgggtgcg
     5821 ccgcggctca gggcgccagc tccaggcgca ggccgtcggg ctgcacgcgc agctggggcg
     5881 gcgcgccgcg cccggccgcg cgcagggtgg ccaggcgttc gggcggcagg cggtagatga
     5941 ccagcccttc cagcagctgc tcgaccacgg gcggggcgtg gcgcgccagc agcgtctgcg
     6001 cggcgggcgg cagctggtcg aggccgaggc gctgcacgcg caccgcgctc aggcgcacgg
     6061 cgccctgctg caggtcgagc tgcagggcgc tgtcgagctc gatggcgccg gtgtagcggc
     6121 gcccgcccag gcgctcggtc agcaccagat cgagcgcggt ggccacgcgg ttggccgccg
     6181 gcagcaggct caggcgcggc gagtgcaggg tcagatcggc caggccggcc aggctgcggg
     6241 tgaccggaaa gcgccgcgcc agcagctcgt ccagccgcgc gacgggcagc agccagccgc
     6301 cggacgcgcc gccggcgcag gccagcaggg ccggcagcag cgggctcagc agggccgcgc
     6361 cgagcaggcg ctggcaggcg cggcgacggt ctggggcgga tggcggtggc atggcgcaca
     6421 agatacccga agcgccggcg gcaggcgact gccgacaccc aggcttaagc gcccaatcgg
     6481 ccgttggggc ttatccatcc tgcgccaaca gctatccaat cgatagtcat ggaagctcag
     6541 gtgagcggcc gcacgccggg ccggccgatg gattcgtaag ccaggccgtg gcgcgccagc
     6601 agggccggtt cgtacaggtt gcggccgtcg aacacgatgc ggtcggccag ccgctgtgcc
     6661 agctggtcga agtcgggcac gcgaaactgc ttccactcgg tgacgatggc cagcgcatcg
     6721 gcgccggtca gcgcctcgga cgggctgtcg cacagcacca ggtcgggccg ggcgccgaag
     6781 atgcggcgcg cctcgtccat ggccaccggg tcgtgcgcgc gcacccgggc gccggcggcc
     6841 cacagctggg ccagcagggt gcggcttggc gcctcgcgca tgtcgtcggt gtcgggcttg
     6901 aaggccaggc cccagacggc gatggtcttg cccgccagcg tgccaccgta gtgcgccacg
     6961 atgcgctggc cgagccgctg gcgctgcgcc tggttgcgcc gctccaccgc ctgcagcagc
     7021 tcgggctcga agccgaccga ctgcgcggtg tggatcagcg cctgcacgtc cttgggaaag
     7081 cagctgccgc cgtagcccat gccggggtag atgaagtggt agccgatgcg cgggtcgctg
     7141 ccgatgccgc ggcgcacggc ctcgacgtcg gcgcccaggc gctcggccag gctggcgatt
     7201 tcgttgatga agctgatctt ggtggccagc atggcgttgg cggcgtactt ggtcagctcg
     7261 gcgcttttca cgtccatcac caccatgcgg tcgtggttgc ggttgaaggg cgcgtacagc
     7321 tcgcgcaact gcgcctcggc ggcggcgctt gcggtgccga tgacgatgcg gtcgggccgc
     7381 tggcagtcgg ccacggccgc gccttccttg aggaattcgg ggttggagac gacgtcgaag
     7441 gcgatctgca ccccgcgctc gcgcagcacg gcgcgcacca cctcggccac gcggtcggcg
     7501 gtgcccaccg gcacggtgga cttgttgacc agggtcttgg gcaccgtcat gtgggtggcg
     7561 atgctgcgcg ccaccgccag cacatgctgc aggtcggcgc tgccgtcttc gtcgggcggg
     7621 gtgccaacgg ccaggaacag cagctcgcca tgcgccacgc cctcggcggc gtcggtggaa
     7681 aagcgcagcc gcccggccgc ctggccggca cgcaccaggg cgtccaggcc gggttcgtgg
     7741 atcgggatgc ggccggcgcg cagcgccgcc accttggcct cgtcgacgtc gatgcacagc
     7801 acatggtggc cgacgtcggc cagcacggcg ccttgcacca ggccgacgta gccggtgccg
     7861 aatacggtga ctttcatggg cttgggctca ctgaggttgg tcgggggccg gcgcggccgg
     7921 ctgccacagg tactggtaat gcgccggtgg cgtgccgtcg cgcaccatgc gcaagggcag
     7981 ttcgatgctg cgcaccggcc gcgccggcag gcgcgccgtg gccagcgccc accagtcggg
     8041 ctccagccgg tcggtgcgcg acaccagcag ccagccctgg ccggccgccc gggcacgcgc
     8101 caccagggcc gccacgcagt cgctcagcgg gtcgtcgtcc ttgacggtgc aggagccgac
     8161 cgaggcgcgc ggaaagcgcg tgcgcagcag gccggccatc atgtggtcgg cggcaatgat
     8221 ggggctgcgg ccgtcgtagc cggcggcgct gagggcggcg cccagttcgg ccgccgggtg
     8281 gttgagctcg tcgacctcgc cgcgcaggcc actgaaccag ggccgcagac cggccgccag
     8341 caggatcagc agcgccatgg cgacgatggc gccggtgtag cgcgcgccgc gcgggtgctg
     8401 ctgcagctcg ggccgcgccg cgaacgccgc cagcggcacc acgcacagca gcggcagcag
     8461 ccagcggccc ttgaactcgg tcaccccggc cagcaccatg cccagcagcg ccaccagcac
     8521 cagcagcaga tagcggccga acacccgctg cgcccagggc agggccggct gccgcggcga
     8581 gcgccaccag gcgccaccaa acgcccacag cgcgaacagc gcgaacagca gcagggtgcc
     8641 ggccacgccc tgcagcaggt ggcgcgcgcc cttgagcggg ccgcgggcgg cgtcgatctc
     8701 catcttgctc agggtgccgg cggtggcctc ggacaggtgc gacagcagcc agcccaggtg
     8761 cggcagcacc accagcagcc ccaccagcgg cgcccaccac cagccgcgcg acagcagggc
     8821 gcgccgcgcc tgcggctccg acagcgcggc cagcagcatg gcgccggcca ccagggcaaa
     8881 gctgtacttg gccagcatgc ccaggccgca caccaggccc agggcggcga actcgagcgg
     8941 gcgcggctgg cgcacgatgc gcagcagcag ccaccaggca ccgcaggcca tggcggtgac
     9001 cagcacggtg tgggtgtggt cgcgcaccga ataccagccc agggtcggca gcagcatcag
     9061 gctggcggcg gcccaccagg ccccgcgcgg cgccagcagc tcgcgcccgg ccagccacat
     9121 gagggcgtag gtcagcgcca gcagcgcgtg cttgagcagc gacagcgcca gcacgctggg
     9181 gccgaccagc cgattgacgc cccattgcag ccaggtgtag agcggcggct gcgcgccgta
     9241 gcccagcgcc agttgctgcg accacagcat ctgctctgcc tcgtcccact tgagcgccgg
     9301 cgaaatggcc acccgcaccg ccacgtgcgc cagcgccagc gccagcagcc aggccagggg
     9361 atgggtgacg ggcgagcgca caagcggcac gccgggcgcg gcggtgaggt tcatcggagt
     9421 cggggtggca ccgtgccgtc acccgccgtg gcggcgcaag gcacaatcgc gcgattatcc
     9481 cgccagcacc cgccccatga gcaccgaccg ctccacggcg cccgatgtgt ccatcgtggt
     9541 gccgatctac aacgaagtcg acaacctgcc cgatctggtc gaacgcatcg ccgccgccat
     9601 ggccgggcag gcgctcagct tcgagctgct ggcggtggac gatggctcca gcgacggcag
     9661 tcgcgcccgc ctgcgcgagc tggccgccgg ccgcccctgg ctgcgcccgg tgctgctggc
     9721 gcgcaactac ggccagtcga gcgcgctgca ggccggcttc gaccgcgtgc gcgggcgcta
     9781 cgtggtgacg ctggacgccg acctgcagaa cgaaccgggc gacatcccgc tgctgctcga
     9841 acggctcgaa aacgaccccg aggtggacat ggtgagcggc tggcgcaagg accgccagga
     9901 cgccgagctg tcgcgcaaac tgccctcgcg catcgccaac cgcctgattt cgcgcgccac
     9961 cggcgtgcac ctgcacgact acggctgcgc gctcaaggcc taccgccgcc ccatcatcga
    10021 ccgcatccgc ctgtatggcg agctgcaccg cttcatccct tcgctggcca gggaggccgg
    10081 ggcgcgcatc accgaggtgc cggtgcgcca ccatgcgcgc acgcgcgggg tgtccaagta
    10141 cggcatcgac cgcaccttcc gcgtcatcct cgacctgatc ctgatcgtgt tcttcatgcg
    10201 ctaccgccag cgtccgctgc acgcctttgg cgggctgggg ctgtggctgg cggcgccggg
    10261 cgggctgatc ctggtctggc tgctgctgca aaagctgctg ggcgaggaca tcggcgggcg
    10321 gccgctgctg ctggtgggcg tgatgctggt gctgatgggg gcgcagctga tcgccgccgg
    10381 gctgatcggc gagctgctga tccgggtcta ccacgaggcc ggcggggcgc cccagttcca
    10441 tgccaccgag ctgcgtccgg acgatgagcc acaacaggct tcagcgcccg tcccacctgc
    10501 gccgacagct actaataaga tagcgtgaag cgccccctca aggccgtgct gcgggccctg
    10561 ctgggcctgg cgctgctggc cggggtgctg acgctggccg atccggcgcg ggtgctggcg
    10621 cagtggcgtc aggccgatcc ggcctggctg ctggcgggcc tgctggcggc catcggctcc
    10681 aacgcggtgt cggcgctgcg ctggcgcgcg ctggcgcgct ggctgggcgc cgagctgagc
    10741 gcgcgcgagg cggcgcgctg gtacttccag gccatcggcc tgaacaccct gctgccgggc
    10801 gcggtggtgg ggggcgatct gtaccgggcc gtgatgctgc gccgcgccgg gcaggcgacg
    10861 gcggcggccg gctggtcggt gctgctggac cgcctgagtg ggctgtggat gctgtgcgcc
    10921 atcggcgctc tgggggcggc cgcctgtgcg ccggtgctgg ggccctggct gcacctgccg
    10981 ccggcgccgc tggcggcgct gctgctggcc ggcagcgccc tgtggctggc gctgccctgg
    11041 gccttgccgg cgctggggcg cgcgcgcccg ggcagcggcg gctggctggc gccgctgcgg
    11101 ctggcggcgg cgcggcccga ctttcgccgc cagctgctgt ggcaggcgct ggcctcggcg
    11161 gcggtgcagg ggctgtcggc ggcggcgctg gcggccggcg cgctggcgct gggcgtggtg
    11221 cagccgctgg cggtgtgggc ctgggccatc gcgccggtgt tcctgatggc ggcgctgccg
    11281 gtcagcgtgg gcggctgggg cacgcgcgag gcggcggcgg tggcggcgct ggcgccgttc
    11341 ggcgtggcgc cggcggcggc ggtgggggtg ggggtgatct acggcctgta cggcctggcc
    11401 cagggcgcgc tgggcgccct ggccttcggt ctgccgcgag ttgctacgga ataaatagct
    11461 gctggcgctt gaccatcaag cgctggcacc cgttttgact cctgacttgg gtcgggccga
    11521 ggaggcccgc catagcgaac gcagtgcacg cagaaacagc caatgaatgc ttcttgcgcc
    11581 ttcggcgtac ggatgcccag gggttgtcgc ggcggcgccg ccggcgccgg cctcaggcgg
    11641 gcttctgggt cagcacgccg cggcgcatct ggtcgagctc catggtctcg aacagggcct
    11701 tgaagttgcc ttcgccaaag ccttcgttgc ccttgcgctg gatgaactcg aagaagatcg
    11761 ggcccagctg gttctcgctg aagatctgca gcagcagctc gccgtcgtgg ccgtcgacca
    11821 ggatgttgcg cgcctgcagc tcggcgatcg gctccttcag gtccgggatg cgccccggca
    11881 gcagctcgta gtaggtgtcg ctggtgttca gcagcttgac gccggccagg ccgagctggt
    11941 cgaccgcgtg gtagatgtcc ttgcagccca gcgcgatgtg ctgcacaccc tcgcccttgt
    12001 agagatcgag gtactcctgg atctggccct tctgctcgtt gccttcttcg ttgatcggga
    12061 tgcggatctt gccgcagggg ctggtcatgg ccttgctctt gacgccggtg gcctggccct
    12121 cgatgtcgaa gtagcgcacc tcgcggaagt tgaacaggcg ctcgtagaag ctggcccatt
    12181 cgtccatgcg gcccttgtgc acgttgtgcg tgaggtgatc gatcaccgtc atgccgtagc
    12241 cctcggggtt gactgcggcg ccgccctgca cgcccggcag cgggtagaaa tcgacgtcat
    12301 agaagccgat gttgccgaga tcgccctccc gggcaccgtt cttgccgcgc cagcggtcga
    12361 tcaggtagat gatggagtcg ccgatgccct tgatggccgg gatgttcagc tcacccgggc
    12421 cggcctggcc agcgtagccc caggcgccca ggctgatggc gcgctcgtag gccgccttgg
    12481 cgtcctgcac gcgaaaggcg atggcgcaga tgctgggtcc gtgcaggcgc gcaaagcgct
    12541 gcgcaaagct gtcgggctcg gcgttgatga tgaagttgat ctcgccctgg cggtacaggg
    12601 tgacgttctt gtggcggtgg cgcgccacgg cggcaaaacc catgcgctcg aacagcgcgc
    12661 ccatggcctg aggatcggga gcggcgtatt cgatgaactc gaagccatcg gtacccatgg
    12721 ggttgtccca ggtcgtggcg gcggggctgg cggggggcat ggcttgtctc ctgaaagaag
    12781 tggtgggcag gcaaatcatg aactgtagag cgcccgcctg cgcgaattcc agccagttgc
    12841 accggccatt cccgccgttg cgcaaggaaa atgcttgacc aaggtcattt cgcgccggat
    12901 tgatgcaaga atcctcccgt cacgcccgat atcgtccttc caccctgagc ccaccgcagc
    12961 catgaccgcc gccctcgacc gcctcgaccg cgccatcctg gcacgcctgc agaccgacgg
    13021 gcgcgaaacg cacgaggcca tcggccagca ggtcgggttg tcggccagcg ccgtgctgcg
    13081 gcgcatccgg cggctggaag aggccggggt gatcgaccgc tacgtggccc tgctgcggcc
    13141 ggaatcgctc gggctggggc tgagcgccta cgtcaacgta cggctggaaa aacgcgccga
    13201 gcacgccaag cgcaacccga tggacgaatt ccgcgccagc gtgcagggct ggcccgaggt
    13261 ggtggaatgc gtggccctga ccggcgagat ggactttctg ttgcacctgg tggtgcagga
    13321 catgggggcg tactcgcgct tcatgatgga cacgctgctg cgtcacccct cggtgcagga
    13381 ctgcaagacc agcttcgtga tggaccgtgt caagaccacc acggcgctgc cgatctagga
    13441 ggctgtcgaa cgccgttcgg gctgaacctg tcgaagccct tcgacaggtt caggatgaac
    13501 ggcggtgcac cagaagctgc acattcttgc gtctcgcagc gctgcggcgt gatcggttac
    13561 acagtcggcg cgtcagggtg acgattggag ttttctctct agagccgcgt ccctaccatt
    13621 gggtgcatga accagcttca cccacccgtc atcgacaccc cgatcgaccc ggccccgcgc
    13681 cgcccgtccc gacggagcgc cgtggtgccg atccgcgagg tcgggcccga gcaccgggaa
    13741 cgcattgcac gccatctgct ggcgctggac gagcacgacc gctacctgcg cttcggttac
    13801 gctgcgggcg acgcccagat ccgccagtac gtggacggac tgaacttcga gcgcgaccac
    13861 gtgttcggca tcttcaaccg ccgcctgacg ctgatcgcca tggcgcacct ggcctacccg
    13921 aacgatttcg cgtcggccgg ctttgccgag ttcggcgtct cggtggccaa gcacgcccgc
    13981 gggcgcggct acggcaagcg cctgttcgag cgcgccgcca tccacgccgt caacgacggc
    14041 gtgcagaccc tgtacatcca cgccctgagc gagaacaccg ccatgctgcg catcgcgcgc
    14101 caggccggtg cggtggtcga acatgccggc agcgaaagcc aggcccacct gagcctgccg
    14161 ccggccacct tcgccagccg cgtcagcgag ctgctggccg atcaggtcgg tcaggtcgat
    14221 ttcggtctga agaccgccgc ctcgcaggcg cgccagctgc tggccacggt gcaggaggtg
    14281 cgcgcggcgg tgcgcgacgg ccagcatcgg tcggacgtgt gacgtgggtg cgcgttcagc
    14341 gcgtaccgtg aagaccggcg ccgcgccctg aacggcgcct gccggccggc gccggcgcgg
    14401 cggggcacat gggctatcct tgcccttgtc acaacttgag cacgccctgc acccgaccgt
    14461 ggccgacccc ccttccgcgc gcaaccccga acgctcgaac gagcgcgagg acaagcgcag
    14521 cctgtttcag aagctgatcg agttcatccg tcccggcccc gactcgacgc acgagctgat
    14581 cgagacgctg gccgacgccg aggacaagga ggtcatcaac gccgaaagcc gcgtcatgct
    14641 ggaaggcgtg ctgcgcctgt ccgacatgac cgccggcgac gccatggtgg ccgccccgcg
    14701 catggacctg ctggacatcg acgcgcccta tgaacagctg ctgcgcgacg tcatcaccac
    14761 cgcgcattcg cgctttccgg tctaccaggg cgagcgcgag aacatcatcg gcatcctgct
    14821 ggccaaggac ctgctcaagc tccagcgcgc gcccgagctg aacctgcgca cactgctgcg
    14881 gccggccatg ttcatccccg aaagcaaggg cctgagcgac ctgctgcgcg cgtttcgcat
    14941 cagccgcaac cacctggcca tggtggtcga cgagttcggc cgcacggccg gcctgatcac
    15001 catcgaggac gtgctggagc agatcgtcgg cgagatcgag gacgagttcg acatcgacgc
    15061 cgacgagggc gacatcttcg ccctggccga ccacagctgg cgcgtggccg gcgacacgcc
    15121 gctggagcgc gtcaacgaag ccttcggcgt gcgcctgggc gccgacccgc agggcgacct
    15181 ggaggccgag ttcgacaccg tcggcggcct gatcgcgcac gaaatgggcc atgtgcccaa
    15241 gcgcggcgag caccaccgca tgggcacgct ggactttcac gtgctgcaca cgcgcggcgg
    15301 cgcggtgcgc tggttcaagg tggtgcgcgt gccgccgacc gaagcctgat gcgccgggtg
    15361 cacgccctgg cctcgccggt ggcggcgctg ctggccggcg tgctgcaggc gctgtcgatc
    15421 gccgacccct ggcagggcgc gccgcgctgg tggctgcaga ccttcagcct ggcctggctg
    15481 gtgtggcagc tggtgcatca ggccgagcgc cagagctcct ggagcgccag cacctgggag
    15541 cgcccgcaga ccgccccggc ctggcgccgc gcgctggcgc tggccggcct gttcgccctg
    15601 ggctggctgg ccggcagctt ctggtggctg ttcacctcca tgcacaccta tggcggcctg
    15661 cacccgctgc tggcggcggc ggcggtgctg gcgctggcgg gctttctggc gctgtactac
    15721 gtcgtggcgg gcgccgcctt cgtggcgctg gcgccgacgc gccggccggt gcgcgcggcg
    15781 ctgctgttcg ccgcgctgtg gaccctggcc gagctggcgc gcggccgctg gctgaccggc
    15841 tttccctggg gcgcgggcgg ctacgcccag gtggacgggc cgctggcgtt tctggcgcgc
    15901 tacgtggggg tgtacggggt cggcttcgtc ggcgcggcgc tggccgccct gctggcgctg
    15961 gcgccccagg tgcgctggcg ccagccgcgc accctggcgg gcaccgctgc gctgctggcg
    16021 ctggccggca gcctgtgggg cgggcgccag atggcgctgg acacgcccgg cgcgccggcg
    16081 ctgaccagcg cggtcgagct gctgcagggc aacatcccgc aggatcagaa gttcatcccc
    16141 ggcaccggca tggccgatgc gctgcgctgg tacggcgcgc agttgcaagc cagcacggca
    16201 ccgctggtgg tggcgccgga aaccgccatt ccgctgctgc cgcgccagtt gcccgagggc
    16261 tactgggagg cgctgcgggc gcgctacgcc caggggccgc aggcggcgct gctgggcgtg
    16321 ccgctgggcg atctggagca tggctacacc aactcggtgg tcgggctggc gccggggcag
    16381 gcggcgccct accgctacga caagcaccat ctggtgccgt tcggcgagtt cattccgccg
    16441 ctgtttcgct ggttcaccga gctgatgcgg atcccgctgg gcgatttctc gcgcggcgcc
    16501 gtcggccaac tgtcgttcgt gtggcagggc cagcggctgg cgcccaacat ctgctatgag
    16561 gatctgttcg gcgaggagct ggcggcgcgc ttcatcgacc cggccacggc ccccacggcc
    16621 ttcgtcaacc tgagcaacat cgcctggttc ggcgacggcg tggcgatcga ccagcacctg
    16681 gccatcggcc gcatgcgcgc gctggagttc gagcgcccca tgctgcgcgc caccaacacc
    16741 ggcgccaccg ccatcatcga ccaccgcggc caggtgacgc accggctggc gcgcgccacg
    16801 cgcggcgtgc tgcgcggaca gttcgaaggc cgcagccacc tcacgcccta cgccgcctgg
    16861 gccgcgcgtc tggggctgtg gccgctgtgg ctgggctgcg cggcgctggt ggtggccgcc
    16921 ctggcccggc aacgccgcgg ctgaggcgcg agcggcgcgg cgccgggcgc ggccgcagcc
    16981 ctcaggcaca atgcgcggca tggctgattc tttttcctac gaccagttga ttgcctcggg
    17041 ggagggccgc ctgttcgggc ccgaggccgg tcgcctgccg ctgccgccga tgctgatgtt
    17101 cgatcgcatc acgcacatcg acgacgacgg tggcgcgcat gggctgggac gcatcgaagc
    17161 cgagctggac gtcaagccgg gcctgtggtt cttcgcctgc cactttcagg gcgacccggt
    17221 gatgcccggc tgcctggggc tcgatgccat gtggcagctg atcggctttt acctgacctg
    17281 gttgcgcctg ccggggcgcg ggcgcgccct gggcgccggc gaggtcaagt tcaccggcga
    17341 ggtcggcccc gacgtcaagc tggtgcgcta cgaaatcgac atcaagcgcg tcatcaagcg
    17401 caagctcaac atggccatcg gcgacgcccg cctgctggcc gacggcaagg aaatctacgt
    17461 cgccaacgac ctgcgcgtgg gtctgttcac gcgggagggg caagcgtcat gagcacccag
    17521 cggcgcgtcg tgatcaccgg cgcgggcatc gtctcgtgca tcggcaacga tctggacacg
    17581 gtggaagcct cactgcgcgc cgggcgctcg ggcatccggg cggtgcccag attcaccgag
    17641 atgggcctgc gcagccaggt ggccggcgtg cccgagatcg acctggaagc gcgcatcgac
    17701 cgcaaggagc tgcgcttcat gggcgacgcc gccgcctacg cgcagatcgc gctcgcggac
    17761 gccatcgccc aggcgggcct cacccccgcg caggtcagcc acccgcgcac cgggctcatc
    17821 atgggctcgg gcggcggctc gccggccaac cagatcgagg ccgccgacac gctgcgcgaa
    17881 aaaggcatcc gccgcgtcgg gccctaccag gtcacgcgct gcatgagcag cacggtgtcg
    17941 gcctgcctgg ccaccaactt caaggtcaag ggcatcaact actccatcac ctcggcctgc
    18001 tccacctcgg cgcactgcat cggcgcggcg gcgcagcaga tcgcctgggg catgcaggac
    18061 gtgatgttcg ccggcggcgg cgaagagctt tcctggggca tggccatgct gttcgacggc
    18121 atgggcgcca tgtccagcaa atacaacgcc acgcccgaca aggcctcgcg cgcctacgac
    18181 gcccaccgcg acggcttcgt gatcgccggc ggcggcggcg ccgtggtgct ggaaagcctg
    18241 gagcacgccc aggcgcgcgg tgccaccatc ctggccgaag tcgtgggctt cggggccacc
    18301 agcgacggcg aggacatggt ggcgccctcg ggcgacgggg cgattgcctg catgcgccag
    18361 gcgatcgacg ggctggatgc gccgatcgac tacatcaaca cccacggcac ctccacgccc
    18421 gtgggcgaca tgcaggaagt gcgcgccatg cgcgcggtgt tcggcgacgc cgtgccgccg
    18481 ttttccagca ccaagtcgct caccggccat tcgctgggcg ccaccggcgt gcaggaggcg
    18541 atctactgcc tcatcatgct gcaaaagggc ttcatcgccg gctcggtgaa cgtcgagacg
    18601 cccgaccccg agctcggcgc catgccgctg gtgaccgcca cgcgcgacgc ccggctgagc
    18661 accgtgctgt ccaacagctt cggcttcggc ggcaccaacg ccagcctggt gctgcggcgc
    18721 tggccctagt ttactacgtt ttttatagct atcggcgctt gcccatcaag cgctggggcc
    18781 acatttcatt cagaacacta aaataccggg tttgcccgct ccggtacgca ccgccatgct
    18841 cacttttcag caaatcatcc tgtcgctaca gcagtactgg gccgatcagg gctgcgcgct
    18901 gctgcagccg tacgacatgg aagtgggcgc cggcaccagc cacaccgcca cttttttgcg
    18961 cgccatcggc cccgagccct ggcgcgccgc ctacgtgcag cccagccgcc gccccaagga
    19021 cggccgctac ggcgacaacc ccaaccgcct gcagcactac taccagtacc aggtggtgct
    19081 caagccggcg ccggccaaca tcctggagct gtacctgggc agcctgcagg cgctggggtt
    19141 cgacctgaag aagaacgaca tccgcttcgt cgaggacgac tgggagaacc ccacgctggg
    19201 cgcctggggc ctgggctggg aggtgtggct caacggcatg gaagtgaccc agttcaccta
    19261 cttccagcag gtcggcggaa tcgactgccg gcccgccacc ggcgagatca cctacggcct
    19321 ggagcgcctg gccatgtacc tgcagggggt ggacaacgtc tacaacctgg tctggaccga
    19381 agggccggac ggcagcaagc tgacctatgg cgacgtctat caccagaacg aggtcgagca
    19441 gtccacctac aacttcgagc acagcgacgc cgacttcctg ttcaccgcct ttgccgcgca
    19501 cgagcggcag gcgctgcacc tgatcggcca gcagctggcg ctgccggcct atgagcaggt
    19561 gctcaaggcc gcccacacct tcaacctgct ggacgcgcgc ggcgccattt ccgtgaccga
    19621 gcgcgccgcc tacatcggcc gcatccgcac cctggcgcgc gccgtggcgc agagctatta
    19681 cgacagccgc cagcgcctgg gctttccgct ggcaccgcgc gcgtgggtcg agcagatcga
    19741 aaaacccgcc gcatgagcat gaccgatatg accacgcaga acctgcttgt cgagctgttc
    19801 gtcgaggagc ttccccccaa ggccctgcgg cagctgggcg aggcctttgc caccaccctg
    19861 gcggcgcagc tgcaggcgca gggcctgacg gcggcggact cgcgcgtcac gccctacgcc
    19921 agcccgcgcc gcctggcggc gcacatcacg catgtggcgg ccaaggcggc cgaccgcgcg
    19981 gtgcagctca agctgatgcc ggtcagcgtc ggcctggacg cccagggtca ggccacgccg
    20041 gcgctgctca agaagctgca ggcgctgggc gccgagccca cggtggtgcc gcagctcaag
    20101 cgccagccgg acggcaaggc cgaggcgctg ttcctggaca gcgtggtcac cggcgccacg
    20161 ctggacagcg gcttgcagaa ggcgctggac gaagccatcg cgcgcctgcc gatccccaag
    20221 gtcatgacct accagctgca ggccggctgt gcccgcccgg gctgggacag cgtgcagttc
    20281 gtgcgcccgg cgcacggcct ggtggcgctg cacggcgaaa ccgtggtgcc ggtcacggcg
    20341 ctgggcctgc gcgccggccg cgccacgcac ggccaccgct tcgaggccgc cagcgacccg
    20401 gtggagctgg ccagcgccga cgactacgcc ctcgacctgc gccagcaggg cgcggtgatc
    20461 gccagcttcg aggagcgccg cgccaccctc gaagcacaga tcaaagcggc tgcaacgcag
    20521 gcggggcctg cgctgacagc tatcaaagac gaagcattgc tggacgaggt gacggccctg
    20581 gtcgagcgcc ccaacgtgct ggtctgcgcg ttcgagcccg aattcctgca ggtgccgcag
    20641 gaatgcctga tcctgaccat gaaggccaac cagaagtact ttccgctgct ggacgcacag
    20701 ggccggctga cgcaccgctt cctggtggtc agcaacatca cgccggacga cccgagcgcc
    20761 gtgatcgccg gcaacgagcg cgtggtgcgc ccgcgcctgg ccgacgccaa gttcttcttc
    20821 gacc
//
LOCUS       DAOOOG010000026        20811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_2519_length_20811_cov_28.167181, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000026 DAOOOG010000000
VERSION     DAOOOG010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 20811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20811
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2519_length_20811_cov_28.167181"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PK242_03655"
     CDS             complement(<1..148)
                     /locus_tag="PK242_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOZ93143.1"
                     /translation="MNQNKRPNIIYIVADDLGYADLGCYGGRDEPWGPVSPVLDRLAA
                     QGLRF"
     gene            complement(302..1213)
                     /locus_tag="PK242_03660"
     CDS             complement(302..1213)
                     /locus_tag="PK242_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HOZ93144.1"
                     /translation="MSAPAHAQNDAPLRLIVPYAPGGSSDRAARIVADKLTARLGAPV
                     VVENKTGAGGRLAMQQSRGLPASQNVLVLANPATMVVAPLVFKDNGYDPDRDFRPVSQ
                     VSDYEFGVAVSTAVPVKEIGHLLAWLRANPEQANFGVPATGSLPHFFALMTGERAQIK
                     AQVVGYRGSGPLLTDLIGGQIPVAFDTFDTLLPQHEAGKLRILGSSGARRSPFAAGVP
                     TFRESGLDLVASGWNAFFAPASMPAARVASLGRAIREVMQEQDTRRKFHDARMVPVAS
                     SPEQTAAMLKAYRAQWAPVVQKSGYQP"
     gene            1358..1843
                     /locus_tag="PK242_03665"
     CDS             1358..1843
                     /locus_tag="PK242_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOZ93145.1"
                     /translation="MPVARLHQPLTLDDMVLYVLWNIQAGAGRAVVRLCENEFGITRR
                     QWRLLAHLAQHEGLQPSELALHVGLDRARTSRALTGLVHKQLVRRLPRPGNRREATLQ
                     LTAEGAALHARLLPRVADINRLLLSVLSEAEIDQLADALARLQAQARRMGGQANSAEA
                     A"
     gene            complement(2062..2406)
                     /locus_tag="PK242_03670"
     CDS             complement(2062..2406)
                     /locus_tag="PK242_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93146.1"
                     /translation="MNPQDTPDKSADATERTIETARRYANEALDRADAGVRNLREDVQ
                     PAIDALSARVQELAERGKALASQTSAQTREKLNEYSEKTSTYVAQQPLKSMAIAAAAG
                     AALALLLGRRRD"
     gene            complement(2468..3262)
                     /locus_tag="PK242_03675"
     CDS             complement(2468..3262)
                     /locus_tag="PK242_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOZ93147.1"
                     /translation="MDDRQLLRYSRHLLLDDIGVEGQQRLLDAHALIVGAGGLGSPVA
                     LYLASAGVGQLTLVDHDTVDLTNLQRQIMHSQGRIGLPKVASAAEAIQAINPEVRVHT
                     VARRGDLPLFCALAAEADVVLDCSDNFATRQALNQACVASGRPLVSGAAIGFDGQVSV
                     YDLRRPDAPCYACLFPPSQTVEEVRCATMGVFAPLVGIIGTLQAAEALKLLAGIGRSL
                     SGRLLMLDARQMEWTEVRVPRQPACPVCRGRDSVPAASRQADPASS"
     gene            complement(3292..4725)
                     /locus_tag="PK242_03680"
     CDS             complement(3292..4725)
                     /locus_tag="PK242_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOZ93148.1"
                     /translation="MSQKTRIAGWIAVGALAGALTTVSLQTVARGTLAPLPLEELQQL
                     AAVFGMIKSDYVEPVDEKKLITDAISGMVSSLDPHSQYFDKKSFKEFREGTTGRFVGV
                     GIEITQEDGLVKVVSPIEGSPADRAGLKSGDLITKVDDTAVKGLSLNEAVKRMRGEPG
                     TKVLLTILRKDENRTFPVTITREEIRTQSVKAKVVEPGYAWLRLSQFQERTVDDFVTK
                     LEDIYRREPNLKGLVLDLRNDPGGLLDAAVAVSAAFLPEGVTVVSTNGQIEESKSIYK
                     ARPEDYLRRRGVDPLRRLPAALKNVPLVVLVNEGSASASEIVAGALQDHKRAIVMGSQ
                     TFGKGSVQTVRPLGPDTALKITTALYYTPSGKTIQAKGIVPDILIDETAEGSPFAVLR
                     MREADLDKHLNGGEAEKKDPALEKEREAAMKRLEEESRKPAAQRRPPEFGSAQDFPLQ
                     QALAKLKGQPVQVSKTLALRKEEPKTD"
     gene            complement(4863..5609)
                     /gene="gpmA"
                     /locus_tag="PK242_03685"
     CDS             complement(4863..5609)
                     /gene="gpmA"
                     /locus_tag="PK242_03685"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOZ93149.1"
                     /translation="MYKLVLIRHGESTWNLENRFTGWTDVDLTPLGIEQACAAGRLLK
                     AEGYDFDVAYTSVLTRAIRTLHLALDEMNRLWLPTVKHWRLNERHYGALQGLNKAETA
                     RQYGDQQVLVWRRSYDTPPPALTPDDPRCERSDPRYARLNPGEVPLTECLKDTVARVL
                     PFWNESMAPAIRSGKRLLVSAHGNSIRALVKYLDGISDDDIVGLNIPNGIPLVYELDA
                     DLRPLRPHYYLGDADAIARAAAAVASQGRA"
     gene            5786..6190
                     /locus_tag="PK242_03690"
     CDS             5786..6190
                     /locus_tag="PK242_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOZ93150.1"
                     /translation="MDFLLANWMLILVALTSGTMLLLPLLQGGGAGLTPTAAVQLINR
                     DKAVVVDVCGTDEFAAGHVAGARSIPLHELEARLPGSVKNKATPLVLVCASGMRSGRA
                     VAIARKLGYENAQSLAGGLKAWREANLPVERG"
     gene            6218..6475
                     /gene="grxC"
                     /locus_tag="PK242_03695"
     CDS             6218..6475
                     /gene="grxC"
                     /locus_tag="PK242_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HOZ93151.1"
                     /translation="MPTVRMYATATCPYCLRAKQLLAHKGVTAIEEIRVDLEPAQRRA
                     MMALTGRHTVPQIFIGDTHVGGCDDLMALEARGGLDALLAG"
     gene            6596..7048
                     /gene="secB"
                     /locus_tag="PK242_03700"
     CDS             6596..7048
                     /gene="secB"
                     /locus_tag="PK242_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HOZ93152.1"
                     /translation="MADQDPVFNIQRVYLKEASLEQPNSPAILLEQQQPAVEISLGVN
                     AENVADGIFEITVTATVQTKIEDRTVFLCECKQAGIFEVRNVPDEQMGAVIGIACPQI
                     VYPYLRGNVADLIQRGGFPPVHLAEINFQQMYEQQQAAQADGASALPQ"
     gene            7094..8119
                     /locus_tag="PK242_03705"
     CDS             7094..8119
                     /locus_tag="PK242_03705"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOZ93153.1"
                     /translation="MKIIVIGAGAWGTALAVSAARNPAGHQVVLWARDGAQAQAMAEQ
                     RENARYLPGIPLPRELQVRGGEVAGLLQAVPQAELLIVATPVAGLRDTLRLLRGVPVP
                     VGWLCKGFEPAQDPGAAHPFGLMPHEIQRQVAPELRAGALSGPSFAQEVARGQPTALV
                     AASRHADVRDALVAGFHGPTLRVYANDDLIGVEVGGAVKNVLAIATGLADGLNLGLNA
                     RAALITRGLTEMARLGLALGARADTFMGLSGLGDLVLTATGDLSRNRRVGLLLAEGKT
                     LEEAVRSLGHVAEGVYSARTVWQRAQHLGVEMPITESVVALLDGRLKPVEAVAALMGR
                     DPKGELL"
     gene            complement(8498..9595)
                     /locus_tag="PK242_03710"
     CDS             complement(8498..9595)
                     /locus_tag="PK242_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ93154.1"
                     /translation="MFLRIDQVEVRYPGAAQPAVQGATLGLRAGDIGVLIGPSGCGKT
                     TLLRAVAGLEPVSAGTIHLGDTLVSSARVHLAPEQRRIGMVFQDYALFPHLDVAHNVA
                     FGIRHLPAAARAARVAEVLGLVDLAGSQGRYPHELSGGQQQRVALARAMAPAPGLLLL
                     DEPFSNLDVDLRERLAHELRGILKAAGATALFVTHDQLEAFAIGDQIGVMHQGRLHQW
                     DDAYSLYHRPATRFVADFIGHGVFVPAQIVAHEGDGAQLDTPLGPLRHLTDCPLPGAY
                     PQGRCELLLRADDIVHDDAAPVQAQIVRKAFRGSEFLYTLRLVSGETVLAHVPSHHNH
                     RVGEWIGIRPEVDHVVTFPVGADHPVSPGAG"
     gene            9804..11453
                     /locus_tag="PK242_03715"
     CDS             9804..11453
                     /locus_tag="PK242_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOZ93155.1"
                     /translation="MLAFLLRPVPTLLLVLLAGALALPVLVVLGSWVQWDAQTAQILR
                     EMAATVLPEYLWVTVQLALMVGLGVVAMGVPAALLVTVFDFPGRRSFEWLLLLPLAMP
                     AYVTAYAYTDFLQFSGPLQTWLRASFGLQGRLLPEVRSVWGAAWVFAFSLYPYVYLLA
                     RTALVERAPQLMEAARLLGASLPRRLREVALPMARPAVAAGTALALMEVLADYGVSVY
                     FGIQTFTAGIYKAWLSMDNRIAAAQLATVLLALVVLLLWLEQRAQRRLRFASASAARA
                     GSGEARPVRLRGRRAALAWLLCLLPVLLGFVLPVLFMLRPLSADWSVLPWERFLVWAW
                     NSVRLGGMTALLALGAAWLLAFGLRRRPSLLRRGAVQLISLGYAVPGAVVVVGLLLPV
                     GWLQARWPGSGAGALVTASVLGLLWAYLVRFVSVALQSVQSGYARVSHSLDESARLLG
                     ASGLELALQVHWPLLRRAGLAAALLVFVDVMKELPATIVLRPFNTDTLAVVANQLARD
                     ERLGEAALPSLALVLVGLVPVVLLSRTLRAERRAQGDAARV"
     gene            11484..11825
                     /locus_tag="PK242_03720"
     CDS             11484..11825
                     /locus_tag="PK242_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOZ93156.1"
                     /translation="MDPTDPGRPDAALEADQVFELAAEVFRVMSAPMRLKIISALCNG
                     EKNVGQLLAEIRTTQPNMSQHLNTLYQAGVVGKRRDGVQIYYRIINDRVVNLCRAVCT
                     QIAMETDGDPA"
     gene            11971..12282
                     /locus_tag="PK242_03725"
     CDS             11971..12282
                     /locus_tag="PK242_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I cytochrome c"
                     /protein_id="HOZ93157.1"
                     /translation="MKPILLSGFAAAALALVASAAQAQTDPLQVRSWAAACANCHGTN
                     GHAQTGNEPLAGGNKDEMLKKLLDFKRGAKPATIMHQLSKGYSDEQLAAIAAWFAAQK
                     K"
     gene            12294..13568
                     /locus_tag="PK242_03730"
     CDS             12294..13568
                     /locus_tag="PK242_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOZ93158.1"
                     /translation="MDRRQFIQAGSAASVLGLTAGCATTGGGAGGKVVVIGGGYGGAT
                     AAKYIRMWSEGRVEVTLVEPGESFISCPISNLVLGGSKQMADITTPYDNLSRRHGVKM
                     VRDRVSAIDADKRQVRLASGGVLPYDRLVVSPGVDFMWDALPGLKKPGAQDKVLHAWK
                     AGPQTVALRKQLEAMPDGGVYALSIPLAPYRCPPGPYERACQVAHYFSQAKPKSKVLI
                     LDANDDVTSKGPLFKKAWADRYKGIIEYRPKHALTDVDAATGTLKFEFNDDIKAQVLN
                     VIPPMRAGDVAHHAGLTTANKRWCDVDWLTMESKAAKNVHILGDALQIAPAMPKSGHM
                     ANQHGKVAAAAIVALLSGRQPNPLPIYNNTCYSFVSDTDVVHVASVHAYDADKKTMVP
                     VAGSGGVSSAASELEGRYAMAWARNIWADSLA"
     gene            13706..14281
                     /locus_tag="PK242_03735"
     CDS             13706..14281
                     /locus_tag="PK242_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ93159.1"
                     /translation="MLAALALAPALAQDSGEARARKIIGGSCFLCHGAEGESASEVFP
                     RLAGQNAEYIAKQLENFKSGKRKSTAMASMVTELTPEDMVALGRFYAARPPHKEAAQD
                     AALAGVGRYIYQSGNKWSGVPSCASCHGAEGAGSTTLPRLAGQHAAYLETQLKQFSKR
                     ERNNDNAVMHTVVEKMTGLEMAAVSEYLSGK"
     gene            complement(14384..15493)
                     /locus_tag="PK242_03740"
     CDS             complement(14384..15493)
                     /locus_tag="PK242_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HOZ93160.1"
                     /translation="MAIDLTSLVEQHGSGTVLAVGGALIGMLFGFFAQRSRFCLRAAV
                     IEFWRGQFAEKLSVWLLAFAAAVMAVQALILAGALNVSEARQLANRGSLSGALVGGLV
                     FGAGMIMTRGCASRLLVLSANGNLRALLSGLVFAVTAQAALAGALAPLRSEISSWWTV
                     DGGAARDLLARVGAGHADGLAFGGLWLVVAIVFSLRSGNRPWMWIGGIGSGLAVALAW
                     WFTYAVAHASFDPVQMQGLTFSGPSAEWLMRVLASPAPAWGFDAGMLPGVFVGSALGA
                     LVGRDFKVEGFKDGYSMARYIAGAVLMGFGSMLAGGCAVGAGMTGGAIFALTAWLTLA
                     GMWAGGGLMHRLLDEPVQTSQPASQQAPVVLPVAP"
     gene            15539..16525
                     /locus_tag="PK242_03745"
     CDS             15539..16525
                     /locus_tag="PK242_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ93161.1"
                     /translation="MRPVIDRRQLLQRCACLALGGMGAAWARYAEAADTPASAPAMQA
                     REAAPGCWYVEGLSALGSSANQNFISNAGFVVTSSGVVVIDALGSPALAERLLAEIRK
                     VTAQKVTHVIVTHYHADHIYGLQVFEALGARILAHQAAREYLNSDTARLRLQASREEL
                     APWVDARTRLVPATEWIHERRELTVGDTTLVLQPVGPAHTPEDLAVYLPGVKALYAGD
                     LVFRSRIPFVGQADSRNWIVSLDRLLAFDTRVIIPGHGPVSTEARQDMQLTRDYLVYL
                     RKTMGAAARDMTPFDEAYRATDWSMYEHLPLFGPANRMNAYNTYLQMEHEPQ"
     gene            16522..17016
                     /locus_tag="PK242_03750"
     CDS             16522..17016
                     /locus_tag="PK242_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93162.1"
                     /translation="MNTPSRFSPARRHGLQALALLGLGAGWAPPAQAAGKTLPLSASL
                     ADELARALKARQPLLVMVSLHRCPWCEEVRNNYLAPMRAQDGLPVVQLDMLSPRATRD
                     LQGAATTHDALVRAWDVKVAPTVLFLGPRGKEVADRLVGGSPDFYSAYLDRRLALARE
                     AVVA"
     gene            17184..17519
                     /locus_tag="PK242_03755"
     CDS             17184..17519
                     /locus_tag="PK242_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93163.1"
                     /translation="MTRRLPLRLGLIGALLALAGAAAAQVPAIFKDADLKLGEKLIVE
                     HKCNECHARKWADDGKAIYRPQGRVNTPGLLRGMIDQCSLELNLGLFPEEVTAIAAVL
                     NRDHYRFKP"
     gene            17616..18995
                     /gene="soxC"
                     /locus_tag="PK242_03760"
     CDS             17616..18995
                     /gene="soxC"
                     /locus_tag="PK242_03760"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HOZ93164.1"
                     /translation="MPDDQLQPAPASVVGGRLRKAPESFLDAAGIRTVFAEGQRGRRD
                     FIRGAFAAALAGGAAARAGAQPASVGEGDPAILNLPAHSTGLGQPVVTDGYGRPSKYE
                     GNVQRRQSPGLTQTNQASVSFAPLQSLFGIVTPSGVHFERHHQGWWDIDPAQHRLMLN
                     GMVKTPKVFTMDELMRLPSVSRFHFIECGANTGMEWGNVAVPTCQYSHGMLSCSEFTG
                     VPLITLLELAGADLKGGKFVLAEGADGSSMTRTIPMELVKSGQVLVAYGQNGEMLRPE
                     NGYPLRLVVPGVQGVSWVKYLRRIEVGDQPWGSKDEAIHYVDLMPDGTHRQYTSIQEC
                     KSVITTPSGGQVLLDKGFYCISGLAWSGRGKIKRVDVSTDGGRNWRTARLESPVMDKC
                     LTRFNLDWVWDGKPTIVQSRAMDDTGYVQPSMRQLREVRGNRSIYHNNAIQSWLVQEN
                     GEVKNVQVS"
     gene            18979..20052
                     /locus_tag="PK242_03765"
     CDS             18979..20052
                     /locus_tag="PK242_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ93165.1"
                     /translation="MFRFREALVGALIATSVALPALAQDKYPGIGRAATPKEVAAWDI
                     DVRPDFKGLPPGSGSVARGQDLWEERCAVCHGVFGESNTIFSPLVGGTTADDVKTGRV
                     ATLKRADYPGRTTLMKVPTVSTLWDYIHRAMPWDKPKSLKPDEVYALTAFLLNLAHVV
                     PDSFTLSDKNIAEVQQKLPNRNGMSSAHAMWPGAELPGRQAKPDVQAKPCMSNCGPAP
                     VVSSVLPEHARNNHGNLREQNRTVGPQRGSDTTKPEGWLGDAAGPVKVVGSAGADSPN
                     AAAIALTQKHACTACHNQSGKLVGPGFTDIAARHAGKADYLAGKIKAGGSGAWGEIPM
                     PPQELPEADTKAIAAWLAAGAAK"
     gene            20141..20599
                     /gene="soxY"
                     /locus_tag="PK242_03770"
     CDS             20141..20599
                     /gene="soxY"
                     /locus_tag="PK242_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HOZ93166.1"
                     /translation="MQTRRQTLKQSAVVAGLLAGTGLFPQYAQAFNKAAFEAKNLGDA
                     LKALGASGAPAASGSVTLTAPDIAENGAVVPLAVASSLANVKQLVLLVEKNPNALVAV
                     FNVRPEVDASFATRAKMGETSDVYAVAITGDGKAHFAKKEVKVTLGGCGG"
BASE COUNT         3168 a         7289 c         7262 g         3092 t
ORIGIN      
        1 tgaagcgcag gccttgggcc gccagccggt ccagcaccgg cgacaccggc ccccacggct
       61 cgtcacgccc gccgtagcag cccaggtcgg cgtagcccag gtcgtcggcg acgatgtaga
      121 tgatgttggg acgcttgttc tggttcatgg atgcgatgga ggcttgtagg gtcagcgccg
      181 gaaggtcagg cccagggtgg tgcgaatcag tttgagggtt cgagacatgg gtgatgcgcc
      241 gcgatgcggc gagccaggag ggaaggaagc ggggatgggc ggccggcgcc gcccggcggc
      301 gtcagggctg gtagcccgac ttttgcacca ccggcgccca ctgcgcgcgg taggccttga
      361 gcatggcggc ggtctgctcg gggctgctgg ccaccggcac cattcgcgcg tcgtggaact
      421 tgcgccgcgt gtcctgctcc tgcatcacct cgcggatggc ccgccccagg ctggccacgc
      481 gcgcggccgg catgctggcc ggggcgaaga aggcgttcca gccgctggcc accagatcca
      541 gccccgactc cctgaaggtc ggcacgccgg cagcgaaggg cgagcgccgc gcgccggagg
      601 aacccaggat gcgcagcttg cccgcctcgt gctgcggcag cagggtgtcg aaggtgtcga
      661 aggcgaccgg gatctggccg ccgatcaggt cggtcagcag cggccccgag ccgcggtaac
      721 ccaccacctg ggccttgatc tgcgccctct cgccggtcat gagggcgaag aaatgcggca
      781 ggctgccggt ggccggcacg ccgaaattgg cctgttcggg gttggcgcgc agccaagcca
      841 gcaggtgccc gatctccttg accggcacgg ccgtggagac ggcaacgccg aattcgtagt
      901 cgctgacctg cgacaccggc cggaaatccc tgtccgggtc gtagccgtta tccttgaaca
      961 ccagcggcgc caccaccatg gtcgccgggt tggccagcac cagcacgttc tggctggcgg
     1021 gcaggccgcg gctttgctgc atggccaggc ggccgccggc gccggtcttg ttttcgacca
     1081 ccaccggtgc gcccagcctg gcggtcagct tgtcggccac gatgcgggcg gcgcggtcgc
     1141 tggagccacc gggggcgtag ggaacgatca gacgcagggg ggcgtcattc tgagcgtggg
     1201 ccggggcgct catcgcgccc acggccagac tggtgccggc cagcagcagg gatcgtcggt
     1261 tcaaggcttg tctcctgagg gtgcctacta caaaatgagc gactgtagtt tgatgaccat
     1321 caattgacaa aatcaatcat tctcaggatt aacccgtatg cccgtggctc gcctgcacca
     1381 gcccctgacg cttgacgaca tggtgctcta cgtgctgtgg aacatccagg cgggcgccgg
     1441 tcgggcggtg gtgcgtttgt gcgagaacga gttcggcatc acgcggcgcc agtggcgact
     1501 gctggcgcat ctggcgcagc acgagggact gcagccgtcg gagctggcac tgcacgtggg
     1561 cctggatcgc gcgcgcacct cgcgggcctt gaccgggctg gtgcacaagc aactggtgcg
     1621 gcgcctgccc cggccgggca accgacgtga agccacgctg caactgacgg ccgaaggcgc
     1681 ggccctgcac gccaggctgt tgccccgggt ggccgacatc aaccggcttc tgctgtcggt
     1741 gctcagcgag gcggaaatcg accagctggc cgatgcgctg gcccggttgc aggcgcaggc
     1801 gcgccggatg ggaggccagg ccaactccgc cgaagccgcc tgagcttgcc gggcggcgcg
     1861 ccgccgatcc gatttcagtg tttcagtgga ctaggaggct gtcggacttg gaaatcgccg
     1921 gctgcaaatc gaccgcagcg gcccattttt caccggcttt ttgcccaata gctcagctat
     1981 tgggctgcaa atccggcaaa aactggtctc gctggggacg attctcgctt tcggcgctcc
     2041 aagcccgaca gcctcctagg atcagtcgcg ccgtcggccc agcagcaggg ccagggcggc
     2101 gccggcggcg gcggcgatgg ccatggattt gagcggctgc tgggccacgt aggtgctggt
     2161 cttttcgctg tactcgttca gcttctcgcg cgtttgcgcc gaggtctggg acgccagcgc
     2221 cttgccgcgc tcggccagtt cctgcacccg cgccgacagg gcgtcgatgg ctggctgcac
     2281 gtcctcgcgc aggttgcgca cgccggcgtc ggcgcggtcc agggcctcgt tggcgtagcg
     2341 gcgcgcggtt tcgatggtgc gctcggtggc gtcggccgac ttgtcagggg tgtcttgcgg
     2401 gttcatgggc atctcctgtg agggtgcgag gggcaaaggg cgattgttgc acccggcgcg
     2461 gtggcggtca ggacgaagcc gggtcggcct ggcggctggc ggccggcacg ctgtcgcggc
     2521 cgcggcacac cgggcaggca ggctggcgcg gcacgcgcac ctcggtccat tccatctggc
     2581 gcgcgtccag catcagcagc cggccgctca gcgagcgccc gatcccggcc agcagcttga
     2641 gcgcctcggc cgcctgcagg gtaccgatga tgcccaccag cggcgcgaac acgcccatgg
     2701 tggcgcagcg cacctcttcc acggtctggc tgggcgggaa caggcaggca tagcagggcg
     2761 cgtcggggcg gcgcaggtcg tacaccgaca cctggccgtc gaagccgatg gcggcgccgg
     2821 acaccagcgg ccggccgctt gccacgcagg cctggttgag cgcctggcgg gtggcgaagt
     2881 tgtcgctgca gtccagcacc acgtcggctt cggcggccag ggcgcaaaac agcggcaggt
     2941 cgccgcggcg tgccacggtg tgcacacgca cctcgggatt gatggcctgg atggcttcgg
     3001 cggccgaggc caccttgggc aggccgatgc gcccctggct gtgcatgatc tggcgctgca
     3061 ggttggtcag atcgaccgtg tcgtgatcga ccagggtcag ctgcccgacg cccgcgctgg
     3121 ccaggtacag cgccactggc gagcccaggc caccggcgcc gacgatcagc gcgtgcgcgt
     3181 ccagcagccg ctgctgaccc tcgacgccga tgtcgtcgag cagcaggtgg cgcgaatagc
     3241 gcagcagttg ccggtcgtcc atcggcgccc gtcccgcgct gcggcgcggg ctcaatcggt
     3301 cttgggctct tccttgcgca gcgccagcgt cttgctgacc tggaccggct ggcccttgag
     3361 cttggccagc gcctgctgca gcgggaagtc ctgcgcgctg ccgaattcgg gcgggcggcg
     3421 ctgggcggcc ggcttgcgcg attcttcttc gaggcgcttc atggcggctt cgcgctcttt
     3481 ttcgagcgcc gggtccttct tctcggcctc gccgccgttc aggtgcttgt cgagatcggc
     3541 ctcgcgcatg cgcagcacgg cgaacggact gccttcggcg gtttcgtcga tcaggatgtc
     3601 gggcacgatg cccttggcct ggatggtctt gccgctgggc gtgtagtaga gcgcggtggt
     3661 gatcttgagc gccgtgtcgg ggccgagcgg gcgcaccgtc tgcaccgagc ccttgccgaa
     3721 ggtctggctg cccatgacga tggcgcgctt gtggtcctgc agggcgccgg ccacaatttc
     3781 gctggccgag gccgaaccct cgttgaccag aaccaccagc ggcacgttct tgagcgccgc
     3841 cggcaggcgc cgcagcggat cgacgccacg gcggcgcagg tagtcttcgg ggcgggcctt
     3901 gtagatggac ttgctttcct cgatttggcc gttggtcgac accaccgtca caccctcggg
     3961 caggaaggcg gccgataccg cgaccgccgc gtccagcagg ccgcccgggt cgttgcgcag
     4021 atcgagcacc aggcccttca ggttgggctc gcgccggtag atgtcttcca gcttggtgac
     4081 gaagtcgtcc accgtgcgct cctggaactg cgacaggcgc agccaggcgt agccgggctc
     4141 gaccaccttg gccttgaccg actgggtgcg gatttcctcg cgcgtgatgg tgaccgggaa
     4201 ggtgcggttc tcgtccttgc gcaggatggt cagcagcacc ttggtgcccg gttcgccgcg
     4261 catgcgcttg accgcctcgt tcaggctcag gcccttgacg gcggtgtcgt ccaccttggt
     4321 gatcaggtcg cccgacttca ggccggcgcg gtcggccggc gagccttcga tgggcgagac
     4381 caccttgacc agcccgtctt cctgggtgat ttcgatgccg acgccgacga agcggccggt
     4441 ggtgccttcg cggaattcct tgaagctctt cttgtcgaaa tactgcgagt gcggatccag
     4501 gctggacacc atgcccgaga tggcgtcggt gatgagcttt ttctcgtcca ccggctccac
     4561 gtagtcggac ttgatcatgc cgaacaccgc cgccagctgc tgcagctctt ccagcggcag
     4621 cggggccagc gtgccgcgcg ccacggtctg cagcgacacg gtggtcaacg cgccggccag
     4681 cgcgcccacg gcgatccatc cggcaatcct ggttttctga ctcatggtct gggggttcct
     4741 gcgtcgcaga ggctcaatat atacctaacg gcctgcgttg gggtccggcc gggcgtgtga
     4801 agttccgcaa cgcggccgcg ggcgacgcgg gggctgaccg cgccgggcgc ctggatgcgg
     4861 cctcaggcgc ggccctggct ggccaccgcg gcggcggcgc gcgcgatggc gtcggcatcg
     4921 cctaggtagt aatgcgggcg cagcgggcgc agatcggcgt ccagctcgta caccagcggg
     4981 atgccgttgg ggatgttcag gcccacgatg tcgtcgtcgg agatgccgtc caggtacttg
     5041 accagagcgc ggatcgagtt gccgtgcgcc gacaccagca ggcgcttgcc ggagcggatg
     5101 gccggcgcca tggattcgtt ccagaacggc agcacgcgcg ccaccgtgtc cttcaggcac
     5161 tcggtcagcg gcacttcgcc cgggttcagg cgcgcgtagc gtgggtcgct gcgctcgcag
     5221 cgcgggtcgt cgggcgtcag cgccggcggc ggggtgtcgt agctgcggcg ccagaccagc
     5281 acctgctggt cgccgtactg gcgcgcggtt tcggccttgt tcaggccctg cagggcgccg
     5341 tagtggcgct cgttcaggcg ccagtgcttg acggtcggca gccacaggcg gttcatttcg
     5401 tccagcgcca ggtgcagggt gcggatggcg cgcgtcagca cgctggtgta ggcgacgtcg
     5461 aagtcgtagc cctcggcctt cagcagccgg ccggcggcgc aggcctgctc gatgcccagc
     5521 ggcgtcaggt cgacgtcggt ccagccggtg aagcggtttt cgaggttcca ggtcgattcg
     5581 ccatggcgaa tcagcacgag cttgtacatg ggcagcggta cggcagggtc aggcaggtgg
     5641 ggcgggccgt ggcggcgccg gcaaaggccg cgcaggcacg gggcataacc cgccatttta
     5701 gaatccccgt ccgtgcaggc ggccgcgggc cgccccgggg cggccccatg gtcgcccgaa
     5761 caccctcaag agaaggaccg acgacgtgga ttttctgctg gcgaactgga tgctgatcct
     5821 ggtggcgctg acctccggca ccatgctgct gctgccgctg ctgcagggcg gcggcgccgg
     5881 cctgacgccg accgctgcgg tgcagctgat caaccgcgac aaggcggtgg tggtcgatgt
     5941 ctgcggcacc gacgaattcg ccgccggcca tgtggcgggc gcgcgcagca tcccgctgca
     6001 cgagctggag gcgcggctgc ccggcagcgt gaagaacaag gccacgccgc tggtgctggt
     6061 gtgcgcctcg ggcatgcgct ccggccgggc ggtggcgatc gccaggaagc tgggctacga
     6121 gaacgcgcag tcgctggccg gcgggctgaa ggcctggcgc gaggccaacc tgccggtcga
     6181 gcgcggctga gccatggccg tcgatccgag ccccgccatg cccaccgtgc gcatgtacgc
     6241 caccgccacc tgcccctact gcctgcgcgc caagcagctg ctggcgcaca agggcgtgac
     6301 cgccatcgag gaaatccgcg tcgatctgga gccggcgcag cgccgcgcga tgatggcgct
     6361 gaccggtcgc cacaccgtgc cgcagatctt cattggcgac acccacgtgg gcggctgcga
     6421 cgacctgatg gcgctggagg cgcgcggcgg gctggacgcg ctgctggccg gctgagcccc
     6481 tgccggccgg ggtgagcccc tctgagataa tggccggctt ttcacgacgg gcgcgccgcc
     6541 tcggcggccg cgccacgccg gctttcaccc ccttcccatc ccagagagaa atcccatggc
     6601 cgatcaggac cccgtgttca acatccagcg tgtctacctg aaggaggcct cgctggagca
     6661 gcccaattcg cccgccatcc tgctggagca acagcagccg gcggtcgaaa tcagcctggg
     6721 cgtcaacgcc gagaacgtgg ccgacggcat cttcgaaatc accgtcaccg ccaccgtgca
     6781 gaccaagatc gaagaccgga cggtgttcct gtgcgaatgc aagcaggccg gcatcttcga
     6841 ggtgcgcaac gtgcccgacg agcagatggg cgcggtgatc ggcatcgcct gcccgcagat
     6901 cgtctacccc tacctgcgcg gcaacgtggc cgacctgatc cagcgcggcg gcttcccgcc
     6961 cgtgcacctg gccgagatca acttccagca gatgtacgag cagcagcagg cggcccaggc
     7021 cgacggcgcc tcggccctgc cgcagtaagc atcaaatctg cctcccgcgc ttgatgggta
     7081 agcgccagca gctatgaaaa tcatagttat cggtgcgggt gcctggggca cggcgctggc
     7141 cgtcagtgcg gcgcgcaacc cggccggtca ccaggtggtg ctgtgggcgc gcgacggcgc
     7201 gcaggcgcag gccatggccg agcagcgcga gaacgcgcgc tacctgcccg gcattccgct
     7261 gccgcgcgag ctgcaggtgc gcggcggcga agtggccggg ctgctgcagg ccgtgccgca
     7321 ggccgagctg ctgatcgtcg ccaccccggt ggccgggctg cgcgacaccc tgcgcctgct
     7381 gcgcggcgtg ccggtgccgg tcggctggct gtgcaagggc ttcgagccgg cgcaggaccc
     7441 cggcgcggcg caccccttcg gcctgatgcc gcacgagatc cagcgccagg tggcgcccga
     7501 gctgcgcgcc ggcgcgctca gcggacccag ctttgcccag gaagtggcgc gcggccagcc
     7561 cacggcgctg gtggcggcca gccgccatgc cgacgtgcgc gacgccctgg tggcgggttt
     7621 tcacggcccc acgctgcgcg tgtacgccaa cgacgacctg atcggcgtcg aagtgggcgg
     7681 cgcggtcaag aacgtgctgg ccatcgccac cggcctggcc gacggcctga acctggggct
     7741 gaacgcacgc gccgccctca tcacccgcgg gctgaccgaa atggcgcgcc tggggctggc
     7801 gctgggcgcg cgcgccgaca ccttcatggg tctgtcgggt ctgggcgatc tggtgctgac
     7861 ggccaccggc gatctgagcc gcaaccgccg cgtcggcctg ctgctggccg agggcaagac
     7921 gctggaggag gcggtgcgct cgctcggcca cgtggccgag ggcgtgtaca gcgcgcgcac
     7981 cgtgtggcag cgcgcgcagc acctgggggt ggagatgccg atcaccgaaa gcgtggtggc
     8041 gctgctggac ggccgcctca agccggtgga agcggtggcg gcgctgatgg ggcgcgatcc
     8101 caagggcgag ctgctctgag cgtggcgcat cacctggctg cgcttcgatg cctggggcgg
     8161 tctcgggcgg ggtgccgggt tcctggtcgg atctgacccc ggcgcgcttg cagtcagcgc
     8221 cggcagctcc tgaattggga gcaagcaggc tcagcctgga aacggcggtt gaccgcttgt
     8281 caacctcggg cagaccgcat cgatgcgggg tcgggcggct gcgatggcca gcacctcctg
     8341 ggtgagcacg ctgcgcaccc gcccgcaccc gcccgcaccg cgctcgccgc gccatgcggc
     8401 gttgctcctt cttgcgcgca ccagcccgcg gcggcgtcgc gtcttgcctg gcacgccgat
     8461 cacggcacgg gcgggggcgt ccgaccgcct cctgggctca gccggcgccg gggctgaccg
     8521 ggtggtcggc gccgaccggg aaggtcacca cgtggtccac ctcgggccgg atgccgatcc
     8581 attcgccgac gcggtggttg tggtggctgg gcacatgcgc cagcaccgtc tcgccgctga
     8641 ccagccgcag ggtgtagagg aactccgagc cgcgaaaggc cttgcgcacg atctgggcct
     8701 gcaccggcgc ggcgtcgtcg tgcacgatgt cgtcggcgcg cagcagcagc tcgcagcggc
     8761 cctgcgggta ggcgccgggc agcgggcagt cggtcaggtg gcgcagcggg cccagcgggg
     8821 tgtccagttg cgcaccgtcg ccttcgtggg cgacgatctg cgccggcacg aacacgccgt
     8881 ggccgatgaa gtcggccacg aagcgcgtgg ccggccggtg gtagaggctg taggcgtcgt
     8941 cccactggtg cagccggccc tggtgcatga cgccgatctg gtcgccgatg gcgaacgcct
     9001 ccagctggtc gtgggtgacg aacagggcgg tcgccccggc cgccttcagg atgccgcgca
     9061 gctcgtgcgc caggcgctcg cgcaggtcca catcgaggtt ggaaaacggc tcgtccagca
     9121 gcagcaggcc cggcgccggc gccatggcgc gcgccagcgc cacgcgctgc tgctgtccgc
     9181 ccgacagctc gtgggggtag cggccctggc tgccggccag atcgaccagg cccagcacct
     9241 cggccacgcg cgccgcacgc gcggcggccg gcaggtggcg gatgccgaag gccacgttgt
     9301 gcgccacgtc caggtgcgga aacagcgcgt agtcctgaaa caccatgccg atgcggcgct
     9361 gctcgggcgc caggtgcacg cgggcgctgc tgaccagcgt gtcgcccaga tggatggtgc
     9421 cggcgctgac cggctccagc cccgccaccg cgcgcagcag cgtggtcttg ccgcagcccg
     9481 aagggccgat cagcaccccg atgtcgcccg cgcgcagccc cagcgtggcg ccctgcacgg
     9541 cgggttgtgc ggcgccggga tagcgcactt cgacctggtc gatgcgtagg aacatggtgg
     9601 gcagcgattg tagatacgta caattctcat tcagggaggc gagggccgcc gcaccccatg
     9661 ccgcagccgc ggcgcgcggc aggccgccgc gggggcgtcc catcgcccgc cagcggccac
     9721 gccgcgcgtt cggggtcaga atggcctctg gcgcgcacct gacctgcgcc agcagctata
     9781 catttgataa caatctgacc atcgtgctgg cattcctttt gcgccccgtc cccaccctgc
     9841 tgctggtgct gctggcgggc gcgctggcgc tgccggtgct ggtggttctg ggctcatggg
     9901 tgcagtggga cgcccagacg gcgcagatcc tgcgcgagat ggccgccacc gtgctgcccg
     9961 aatacctgtg ggtgaccgtg cagctggcgc tgatggtggg cctcggcgtg gtggccatgg
    10021 gcgtgccggc ggcgctgctg gtgaccgtgt tcgacttccc cggccggcgc agcttcgaat
    10081 ggctgctgct gctgccgctg gccatgccgg cctacgtcac cgcctatgcc tacaccgact
    10141 tcctgcagtt ctccgggccg ctgcagacct ggctgcgcgc cagcttcggg ctgcagggcc
    10201 ggctgctgcc cgaggtgcgc agcgtgtggg gcgcggcctg ggtgttcgcg ttctcgctct
    10261 acccctacgt ctacctgctg gcgcgcaccg cgctggtcga gcgggcgccg cagctgatgg
    10321 aagcggcgcg cctgctgggc gccagcctgc cgcgccggct gcgcgaagtg gcgctgccga
    10381 tggcgcgccc ggcggtggcc gccggcaccg cgctggccct gatggaagtg ctggccgact
    10441 acggcgtgtc ggtgtacttc ggcattcaga ccttcaccgc cggcatctac aaggcctggc
    10501 tgtcgatgga caaccgcatc gccgccgccc agctggccac ggtgctgctg gcgctggtgg
    10561 tgctgctgct gtggctggag cagcgcgccc agcgccggct gcgctttgcc agcgccagcg
    10621 cggcgcgcgc cggctccggc gaggcgcggc cggtgcgcct gcgcggccgg cgtgccgcgc
    10681 tggcctggtt gctgtgcctg ctgccggtgc tgctcggctt cgtgctgccg gtgctgttca
    10741 tgctgcgtcc gctgtcggcc gactggtcgg tgctgccctg ggagcgcttt ctggtctggg
    10801 cctggaacag cgtgcgcctg ggcggcatga cggcgctgct ggcgctgggc gcggcctggc
    10861 tgctggcctt cggcctgcgc cgccggccga gcctgctgcg gcgcggcgcg gtgcagctca
    10921 tctcgctcgg ctacgcggtg ccgggcgcgg tggtggtggt gggtctgctg ctgccggtgg
    10981 gctggctgca ggcgcgctgg ccgggcagcg gcgccggcgc cctggtcacc gccagcgtgc
    11041 tgggcctgct gtgggcctac ctggtgcgct tcgtgtcggt ggcgctgcag tcggtgcaaa
    11101 gcggctatgc gcgggtgtcg cacagcctgg acgaatcggc gcgcctgctg ggcgccagcg
    11161 ggctggagct ggcgctgcag gtgcactggc cgctgctgcg ccgcgccggc ctggcggcgg
    11221 cgctgctggt gttcgtcgac gtcatgaagg agctgcccgc caccatcgtg ctgcgcccct
    11281 tcaacaccga caccctggcg gtggtggcca accaactggc gcgcgacgag cgcctgggcg
    11341 aggcggccct gccgtcgctg gcgctggtgc tggtggggct ggtgccggtg gtgttgctga
    11401 gccgcaccct gcgcgccgag cggcgtgcgc agggcgacgc cgcgcgggta tagtcacccg
    11461 gcgccgtgca cagcgaaccc gccatggacc ccaccgaccc cggccgaccc gacgccgccc
    11521 tcgaagccga ccaggtgttc gagctggcgg ccgaggtgtt tcgcgtcatg tcggcgccga
    11581 tgcggctgaa gatcatcagc gcgctgtgca acggcgaaaa aaacgtcggc cagctgctgg
    11641 ccgagatccg caccacccag cccaacatga gccagcacct caacaccctc taccaggccg
    11701 gtgtggtggg caagcggcgc gatggggtgc agatctacta ccgcatcatc aacgaccgcg
    11761 tggtcaacct gtgccgcgcc gtctgcaccc agatcgccat ggaaaccgac ggcgacccgg
    11821 cctgacgcgc ggcaccgggc cgtgaagctg ccttcggggt taatcctgac caaggtcatg
    11881 cttcgagcgc cctatcatgc ggcgaccata taagcggttg cgaattttcc cttctgcccc
    11941 cggcagtccc aaccaggagg acccacgacc atgaaaccca ttttgctttc cggcttcgcc
    12001 gcggcggccc tggcgctggt cgccagcgcc gcgcaggcgc agaccgatcc gctgcaggtg
    12061 cgcagctggg ccgccgcctg cgccaactgc cacggcacca acggccatgc ccagaccggc
    12121 aacgagccgc tggccggcgg caacaaggac gagatgctga agaagctgct cgacttcaag
    12181 cgcggcgcca agcccgccac catcatgcac cagctcagca agggctacag cgacgagcag
    12241 ctggccgcca ttgcggcctg gttcgcggcg cagaagaaat gaggaggcac accatggatc
    12301 gtcgtcagtt cattcaggcc ggttcggccg cttccgtgct gggcctgacc gcgggctgtg
    12361 ccaccaccgg cggcggtgcc ggtggcaagg tggtggtcat cggtggcggc tacggtggcg
    12421 ccaccgcggc caagtacatc cgcatgtggt ccgagggccg tgtcgaggtc acgctggtcg
    12481 agccgggtga gagcttcatc tcctgcccga tctccaacct ggtgctgggc ggcagcaagc
    12541 agatggccga catcaccacc ccctacgaca acctgagccg ccgccatggc gtgaagatgg
    12601 tgcgcgaccg ggtcagcgcc atcgacgccg acaagcgcca ggtgcggctg gccagcggcg
    12661 gcgtgctgcc ctatgaccgg ctggtggtgt cgcccggtgt cgatttcatg tgggatgccc
    12721 tgccgggatt gaagaagccc ggcgcccagg acaaggtgct gcacgcctgg aaggccggcc
    12781 cgcagacggt ggcgctgcgc aagcagctcg aagccatgcc cgacggcggc gtgtacgccc
    12841 tcagcatccc gctggcgccc taccgctgcc cgcccggccc gtacgagcgc gcctgccagg
    12901 tggcgcacta cttcagccag gccaagccca agtccaaggt gctgatcctg gacgccaacg
    12961 acgacgtcac ctccaagggg ccgctgttca agaaggcctg ggccgaccgc tacaagggca
    13021 tcatcgaata ccggcccaag cacgcgctga ccgacgtcga tgccgccacc ggcacgctca
    13081 agttcgagtt caacgacgac atcaaggccc aggtgctgaa cgtcatcccg cccatgcgcg
    13141 ccggcgacgt ggcgcaccac gccgggctga ccaccgccaa caagcgctgg tgcgacgtcg
    13201 actggctgac catggagtcc aaggccgcca agaacgtcca catcctgggc gacgcgctgc
    13261 agatcgcccc ggccatgccc aagtccggcc acatggccaa ccagcacggc aaggtggcgg
    13321 ccgccgccat cgtggccctg ctgtcgggcc gccagcccaa cccgctgccg atctacaaca
    13381 acacctgcta cagcttcgtc agcgacaccg acgtggtgca tgtggccagc gtgcacgcct
    13441 acgacgccga caagaagacc atggtgccgg tggccggctc gggcggcgtg tcgagcgccg
    13501 ccagcgagct ggaagggcgc tacgccatgg cctgggcgcg caacatctgg gccgatagcc
    13561 tggcatgacc cccccgagtc gctgcgcgcc ttcccccctg cgagggggga caacacctgt
    13621 ggccggcgca ggtccggcca cggtgtttct ggcgtggcct gctccgcggc cttctgtccg
    13681 atggctgcgg cgcgtcggtg tcgcggtgct ggccgccctg gccctggcgc ccgcactggc
    13741 gcaagacagc ggtgaggcgc gcgcgcgcaa gatcatcggc ggctcgtgct ttctgtgcca
    13801 cggcgccgaa ggcgaatcgg ccagcgaggt gtttccgcgc ctggccggcc agaacgccga
    13861 gtacatcgcc aagcagctgg aaaacttcaa gagcggcaag cgcaagagca cggccatggc
    13921 cagcatggtg accgagctga cgcccgagga catggtggcg ctggggcgct tctacgccgc
    13981 ccgcccgccg cacaaggagg cggcgcagga cgccgcgctg gccggcgtgg ggcgctacat
    14041 ctaccagtcg ggcaacaagt ggagcggcgt gccttcgtgc gccagctgtc acggtgccga
    14101 aggggctggc agcaccacct tgccgcgtct ggccgggcag cacgccgcct acctggaaac
    14161 ccagctcaag cagttcagca agcgcgagcg caacaacgac aacgccgtca tgcacaccgt
    14221 ggtcgagaag atgaccggcc tcgaaatggc ggcggtgtcg gaatacctga gcggcaagta
    14281 accccaggtt aactcctgaa agtatagctg ctgacgcatg cgggacgcgc gccagcagcc
    14341 ttttttgctt tgaagcccca aaaggcccgc aggggcccgg cgttcagggt gccaccggca
    14401 gcaccaccgg tgcctgctgg ctggccggct ggctggtctg caccggctcg tcgagcagcc
    14461 ggtgcatcag gccgccgccg gcccacatgc cggccagcgt cagccaggcg gtgagggcga
    14521 agatggcgcc gcccgtcatg cccgcgccga cggcgcagcc accggccagc atgctgccaa
    14581 agcccatcag cacggcgccg gcgatgtagc gcgccatgct gtagccgtcc ttgaagcctt
    14641 ccaccttgaa atcgcgcccc accagcgcgc ccagcgccga gcccacgaac acgcccggca
    14701 acatgccggc gtcaaagccc caggctggcg ccgggctggc cagcacgcgc atcagccatt
    14761 cggccgacgg gccgctgaag gtcaggccct gcatctgcac cgggtcgaag ctggcgtgcg
    14821 ccacggcata ggtgaaccac caggccagcg ccaccgccag cccgctgccg atgccgccga
    14881 tccacatcca ggggcggttg ccgctgcgca gcgaaaagac gatggccacc accagccaca
    14941 ggccgccgaa cgccaggcca tcggcgtggc ccgcgcccac gcgcgccagc agatcgcgcg
    15001 cggcgccgcc gtccaccgtc caccagctcg agatctcgct gcgcagcggc gccagggcgc
    15061 cggccagcgc cgcctgggcg gtgaccgcga acaccagccc cgacagcagc gcgcgcaggt
    15121 tgccgttggc cgacagcacc agcagccggc tggcgcagcc gcgcgtcatg atcatgccgg
    15181 cgccaaacac cagcccgccc accagcgcgc ccgacaggct gccgcggttg gccagctggc
    15241 gcgcctcgct cacgttcagc gcgccggcca ggatcagcgc ctgcaccgcc atcaccgccg
    15301 ccgcgaaggc cagcagccag accgacagct tttcggcgaa ctggccgcgc cagaactcga
    15361 tcacggcggc gcgcaggcaa aagcgcgaac gctgggcgaa aaatccgaac agcatgccga
    15421 tcagcgcgcc gccgacggcc agcaccgtgc cgctgccgtg ctgctcgacc aggcttgtca
    15481 agtcgatggc cattcttgat aattagttca gcaatgactt atgaatttag ggcagatcat
    15541 gcggccagtg attgaccggc gtcaacttct ccagcgctgc gcctgtctgg cgctgggcgg
    15601 catgggggca gcctgggcgc gctacgccga agccgccgac acccccgcca gtgcgccggc
    15661 gatgcaggcg cgcgaggccg cgcccggctg ctggtacgtc gaaggcctgt cggccctggg
    15721 ctcgtcggcc aaccagaact tcatctccaa cgccggcttc gtggtcacgt cgagcggtgt
    15781 ggtggtgatc gacgccctgg gctcacccgc gctggccgag cgcctgctgg ccgagattcg
    15841 caaggtgacg gcgcagaagg tcacgcacgt catcgtcacc cactaccacg ccgaccacat
    15901 ctacggcctg caggtgttcg aggcgctggg cgcgcgcatc ctggcgcacc aggcggcgcg
    15961 cgaatacctc aactccgaca ccgcgcgcct gcgtctgcag gcctcgcgcg aggaactggc
    16021 gccgtgggtc gatgcccgca cccgcctggt gcccgccacc gaatggatcc acgagcggcg
    16081 cgagctgacc gtcggcgaca ccacgctggt gctgcaaccg gtcggcccgg cgcacacgcc
    16141 ggaggacctg gcggtgtacc tgccgggggt caaggcgctg tacgcgggcg atctggtgtt
    16201 tcgcagccgc attcccttcg tcggccaggc cgacagccgc aactggatcg tctcgctcga
    16261 tcgcctgctg gccttcgaca cccgggtcat catccccggc cacggcccgg tatcgaccga
    16321 ggcgcgccag gacatgcaac tcacgcgcga ctacctggtc tatctgcgca agaccatggg
    16381 cgccgccgcg cgcgacatga ccccgttcga tgaagcctac cgggccaccg actggagcat
    16441 gtacgaacac ctgccgctgt tcggcccggc caaccgcatg aacgcctaca acacctacct
    16501 gcagatggag cacgaaccgc aatgaacacg ccgtcccgct tctcgcccgc gcgccgccat
    16561 ggcctgcagg cgctggccct gctgggcctg ggtgccggtt gggcgccgcc ggcccaggcg
    16621 gccggcaaga cgctgccgct gtcggcttcg ctggccgacg agctggcgcg cgcactgaag
    16681 gcgcgccagc cgctgctggt gatggtcagc ctgcaccgct gcccctggtg cgaagaggtg
    16741 cgcaacaact acctggcacc catgcgcgcc caggacggcc tgccggtggt gcagctcgac
    16801 atgctcagcc cgcgcgccac gcgcgacctg cagggcgcgg ccaccacgca cgacgcgctg
    16861 gtgcgcgcct gggacgtcaa ggtcgcgccc acggtgctgt ttctcggccc gcgcggcaag
    16921 gaggtcgccg accggctggt gggcggctcg cccgacttct attccgccta cctcgaccgc
    16981 cgcctggcgc tggcccgtga agccgtggta gcctaggctg tcaggcactt attgaagcca
    17041 gacaaaggct tgcggcaagg cgcggctcgc agcccatgtt ggcggctttg aagtgtgttt
    17101 ttgggctgcg gcgcaatcca ggcaagcgcc gacagctatt ttaaggatag caaaccaacg
    17161 ccttgccgga ccggagacaa gccatgaccc gtcgccttcc cctgcgcctc ggcctgatcg
    17221 gtgcgctgct cgccctggcc ggcgcggcgg cggcccaggt gccggcgatc ttcaaggacg
    17281 ctgacttgaa gctgggcgaa aagctgatcg tcgagcacaa gtgcaacgaa tgccatgccc
    17341 gcaaatgggc cgacgacggc aaggccatct accggccgca gggtcgcgtc aacacccccg
    17401 ggctgctgcg cggcatgatc gatcagtgca gcctggagct caacctcggc ctgttcccgg
    17461 aagaagtgac cgccatcgcg gcggtgctca accgcgacca ctaccggttc aagccctgac
    17521 cccgtccacc cagcggcgat caggataaac cccagtcccg acatgtgtat gccgggctta
    17581 acatcagcac ttgcgcatat atagaactta aacccatgcc cgacgatcaa ttgcaacccg
    17641 cgcccgccag cgttgttggc gggaggctgc gcaaggcgcc cgagagcttt ctcgacgccg
    17701 ccggcatccg caccgtgttc gccgaaggcc agcgcggccg gcgcgacttc atccgtggcg
    17761 ccttcgcggc cgccctggcc ggtggcgccg ccgcccgcgc cggcgcccag ccggcgtcgg
    17821 tgggcgaggg cgaccccgcc atcctcaacc tgccggcgca cagcaccggc ctgggccagc
    17881 cggtggtgac cgacggctac ggaaggccgt ccaaatacga gggcaacgtg cagcgccgcc
    17941 agagcccggg cctcacgcag accaaccagg cctcggtgtc gttcgcgccg ctgcagtcgc
    18001 tgttcggcat cgtcacgccc agcggcgtgc acttcgaacg ccatcaccag ggctggtggg
    18061 acatcgaccc ggcccagcac cgcctcatgc tcaacggcat ggtcaagacg cccaaggtgt
    18121 tcaccatgga cgagctgatg cgcctgccca gcgtgtcgcg ctttcacttc atcgaatgcg
    18181 gcgccaacac cggcatggaa tggggcaacg tcgccgtgcc gacctgccag tactcgcacg
    18241 gcatgctgtc gtgcagcgaa ttcaccggtg tgccgctgat caccctgctg gagctggccg
    18301 gcgccgacct caagggcggc aagttcgtgc tggccgaagg cgccgacggc tcctccatga
    18361 cgcgcaccat ccccatggag ctggtcaaga gcggtcaggt gctggtggcc tacggccaga
    18421 acggcgaaat gctgcgcccc gaaaacggct acccgctgcg cctggtggtg ccgggcgtgc
    18481 agggcgtcag ctgggtcaag tacctgcgcc gcatcgaggt cggtgaccag ccctggggca
    18541 gcaaggacga agccatccac tacgtcgatc tgatgcccga cggcacgcac cgccagtaca
    18601 ccagcatcca ggaatgcaag agcgtcatca ccaccccctc gggcggccag gtgctgctgg
    18661 acaagggctt ctattgcatc agcggcctgg cctggtcggg gcgcggcaag atcaagcggg
    18721 tcgatgtctc caccgacggc gggcgcaact ggcgcacggc gcggctggaa agcccggtga
    18781 tggacaaatg cctgacgcgc ttcaacctcg actgggtgtg ggacggcaag cccaccatcg
    18841 tgcaaagccg cgccatggac gacaccggct acgtgcagcc cagcatgcgc cagctgcgcg
    18901 aagtgcgcgg caaccgctcc atctatcaca acaacgccat ccaatcgtgg ctggtgcagg
    18961 aaaacggcga ggtcaagaat gttcaggttt cgtgaggccc tcgtcggcgc cctgatcgcc
    19021 accagtgtcg cgctgccggc gctggcgcag gacaaatacc ccggcatcgg ccgcgccgcc
    19081 acgcccaagg aagtggcggc ctgggacatc gacgtgcggc ccgacttcaa gggcctgccg
    19141 cccggcagcg gcagcgtcgc ccgcggccag gacctgtggg aagagcgctg cgccgtctgc
    19201 cacggcgtgt tcggtgaatc caacaccatc ttcagcccgc tggtgggcgg caccaccgcc
    19261 gacgacgtca agaccggtcg cgtggccacc ctcaagcgcg ccgactaccc cggccgcacc
    19321 acgctgatga aggtgcccac ggtgtccacg ctgtgggact acatccaccg cgccatgccc
    19381 tgggacaagc ccaagtcgct caagcccgac gaggtctacg cgctcaccgc cttcctgctc
    19441 aacctggccc atgtggtgcc ggacagcttc accctgtccg acaagaacat cgccgaagtg
    19501 cagcagaaac tgcccaaccg caacggcatg agcagcgccc acgccatgtg gcccggcgcc
    19561 gaactgccgg gccgtcaggc caagcccgac gtgcaggcca agccctgcat gagcaactgc
    19621 ggcccggcgc cggtggtcag ctcggtgctg cccgaacacg cgcgcaacaa ccacggcaac
    19681 ctgcgcgagc aaaaccgcac cgtcggcccg cagcgcggct ccgacaccac caagcccgaa
    19741 ggctggctgg gcgacgccgc cgggccggtc aaggtggtcg gctccgccgg cgcggacagc
    19801 cccaacgccg ccgccatcgc cctgacgcag aagcacgcct gcaccgcctg tcacaaccag
    19861 agtggcaagc tggtcggccc cggcttcacc gacatcgccg ccaggcacgc cggcaaggcc
    19921 gactacctgg ccggcaagat caaggccggt ggctccggcg cgtggggcga aatccccatg
    19981 ccgccgcagg agctgcccga agccgacacc aaggccatcg cggcctggct ggcggccggt
    20041 gccgccaaat gaaatgaccc gcggaaccat tcgggaattc acccgggtca aaatcccctc
    20101 aaccccttag catccgcaaa ctttcaagga gaaatcgccc atgcaaacac gcaggcaaac
    20161 cctcaagcag agcgccgtgg tggcgggcct gctggccggc actggcctgt tcccgcagta
    20221 cgcgcaggcg ttcaacaagg cggcgttcga ggccaagaac ctgggcgacg cgctcaaggc
    20281 cctgggcgcc agcggtgccc ctgcggccag cggctcggtc accctgaccg cgcccgacat
    20341 cgccgagaac ggcgccgtgg tgccgctggc cgtggccagc agcctggcca acgtcaagca
    20401 gctggtgctg ctggtcgaga agaaccccaa cgccctggtg gcggtgttca acgtcaggcc
    20461 cgaggtcgac gccagcttcg ccacccgcgc caagatgggc gagacctccg acgtctacgc
    20521 ggtggccatc accggcgacg gcaaggccca cttcgccaag aaggaagtca aggtcaccct
    20581 gggtggctgt ggcgggtgac atgacgcccc cctctgtcgc ttcgcgcctt cccccggagg
    20641 gggacaacgc cagtggccgg cgcaggtccg gccacggcgt tcgctggcgc ggcctgctcc
    20701 gcggccttca gaacgggccg ggcgtgcatg ggcgacgagg gtgagataaa ccgaatcgta
    20761 tcgaatcagg agaacagaca tggcagatcc aatgcgaatc cgcgcgcagg c
//
LOCUS       DAOOOG010000027        20616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_2543_length_20616_cov_32.868829, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000027 DAOOOG010000000
VERSION     DAOOOG010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 20616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20616
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2543_length_20616_cov_32.868829"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PK242_03775"
     CDS             complement(<1..316)
                     /locus_tag="PK242_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93167.1"
                     /translation="MPVPTFLAWCATLLLPVVVAHLPLAHAQPAPPHTDAAEFRACLQ
                     GLREAPAFRAVTPATFATHTDGLVPDASVLLLLDRQPEFTLAPWDYLAVLVDAERVAD
                     GRA"
     gene            628..1644
                     /locus_tag="PK242_03780"
     CDS             628..1644
                     /locus_tag="PK242_03780"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOZ93168.1"
                     /translation="MDCFITGTAGFIGFHLAKLLLEQGHTVHGYDGITDYYDVRLKHA
                     RHDLLRQYPQFTATEAMLEDDATLQAAVAAAQPRAIVHLAAQAGVRYSIEHPRSYIDA
                     NIIGSFNVMEAARRQQVAHLLMASTSSVYGANTEMPFDENQKCDMPMSFYAATKKANE
                     AMAHSWAHIHGVPITLFRFFTVYGPWGRPDMALFKFTKAILDGQPIDVYNHGDMWRDF
                     TYVEDLVRAIALLIDAVPPAAAGRNPAQAIEGDSLSPVAPWRVVNIGNSDKVRLLDFI
                     DAIEQCLGQKAIRNHLPIQPGDVPASWADTQLLQRLTGYKPATGFQQGVQAFVRWYCE
                     HHRP"
     gene            complement(1708..3633)
                     /locus_tag="PK242_03785"
     CDS             complement(1708..3633)
                     /locus_tag="PK242_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOZ93169.1"
                     /translation="MSLQGLTHRLIRMPRAGKRLLALTLDAFLCAFTVWLAWALRLEH
                     WVPFGRMHAWATLGSWLLGLPVFAHFGLYASIFRYAGWNATLALGKAVAVYGGLYALV
                     FTLIAVPGIPRSIGIIQPLLMFVALGLSRVTVRLWIGDLYSELLARLNAPGVLIYGAG
                     QSGLQLAASLLRMHQQRLVGYLDDNPALQGRRLDGLPVFAPSDLDRLVREYQVGSVLL
                     AIPSASLARRKQIVELLRPHPVHVRTLPGMADLVRRGVHASDLQDLDIEDLLGRDPVR
                     PDEALVEQQLSGRTVMVTGAGGSIGSELCRQILVWRPRVLLLLEQSEYALYEIHRELL
                     AAMGEPDDADGPLHIVPLLGSVQDAARLDAILTEWHPDTVYHAAAYKHVPLVEHNPIE
                     GLRNNVWGTLECAASAERHGVGHFVLVSTDKAVRPTSVMGASKRLAEMVLQALHAQGE
                     RAGGSGRTCFTMVRFGNVLGSSGSVVPLFRQQIEQGGPVTVTHREITRYFMTIPEAAQ
                     LVIQAGAMACGGEVFVLDMGEPVRIHDLACRMIELSGQRVRRPQDAPDAEGIEIRVTG
                     LRPGEKLYEELLSDDTSVRTAHPRILKAQERFLPWDQLQTWLEQLAEHMRARRHDDIR
                     ALLQQLVEGYRPSDRTD"
     gene            complement(3997..6693)
                     /locus_tag="PK242_03790"
     CDS             complement(3997..6693)
                     /locus_tag="PK242_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HOZ93170.1"
                     /translation="MSIRNLDSLFDPQSVAVIGASERPFSVGGTLWRNMHDGGFAGPV
                     YPVNPKHRTLSGQRCYASVDDLPEAPELAVICTPAHTVVELIGQLAARGTRAAVVISA
                     GLTAEQKQAMLDAARPSLLRVLGPNCVGLLAPHARLNASFAHIPARPGELAFVSQSGA
                     LVTAMLDWAEARQIGFSHFVSLGEHADVDFGDLLDYLASDARTRAILLYIESIDQARK
                     FMSAARAAARNKPVIVIKAGRSAQGQKAAASHTGALAGADDVVDAAIARAGMLRVDSL
                     EELFLAAEILTRQTAPIGDRMTILTNGGGVGVLAADAAAAHDVPLAELSPALVGALDA
                     VLPANWSRGNPVDIIGDAPAQRYVDALQALTRHPQEVGTVLFIQAPTAIVPSTDIARA
                     LLPALRPEGGAPLPLVSCWVGGPAVAEARQLFIEAGIASYDMPEQAVAAIAMLQAYAR
                     NQAELAEAPPAEQLHNGSTPDVARVRALVQAVLASGRDMLTEPEAKAVCEAYHIPVVA
                     TRTTPADPEAAAAEAARIGFPVVIKILSEDISHKSDVGGVVLNLQDEADVRAAASAML
                     ARIRKSQPKARLDGFSVQAMVRRKQAQELIIGASIDPSFGPVILFGQGGTAVEVMKDS
                     AMALPPLNDPLARALIARTRVARLLAGYRDVPAADLDAVVATLMAVSQLLVDVPEVAE
                     LDINPLIVNHEGAIALDARVRVSPRLPAGAAHFAIAPYPAQLIETLEWKGQRLTLRPI
                     RPEDELQHRAFLESLDAEDVRMRLFYSRRTMERSELARLVQIDYTREMAFIATLPAPE
                     GGEQTLGVARALADPDNLGAEFGIILRPELKGSGLGRLLMDKLIRYQRGAGTQRLIAS
                     ALVENWRMRELARALGFAEAPTPGEPDTLRLTLELQAPSAAA"
     gene            complement(7086..9395)
                     /locus_tag="PK242_03795"
     CDS             complement(7086..9395)
                     /locus_tag="PK242_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOZ93171.1"
                     /translation="MNKPLTPAEAALRDAAREYHRNPKPGKIAVTPLKPLSNQLDLSL
                     AYSPGVAYPCLDIQADPSKAAEFTSRGNLVGVVTNGTAVLGLGDIGPLAGKPVMEGKG
                     CLFKKFAGIDVFDIELAERDPDKLVEIIASLEPTLGGINLEDIKAPECFYIERKLRER
                     MNIPVFHDDQHGTAIISAAALLNGLELVGKQIGDVKVAVSGAGAAAIACLDVMVALGV
                     KPAHVFACDSKGLIYHGRAGGFDESKARYAQPDTGARTLADVVKGADVFLGCSAPGVL
                     TGEMVKTMAAKPVILALANPEPEIRPEDAKAARPDCIIATGRSDYPNQVNNVLCFPYI
                     FRGALDCGASKITEEMKLACVHEIAALAKAETSAEVAAAYAGKDLQFGPDYIIPTPFD
                     PRLIMRIAPAVARAAFESGVASRPISDMAAYRASLERFVYQTGMVMRPVYAAAKAVAP
                     EAKRVAYAEGEDERVLRAAQIVLDDGIATPILIGRPAVIEQRIAKAGLRMRLGQDVEC
                     VNPEDDRRFRQYWETYHGLMARGGVTVDAAKAAVRRSTTIIAALMVHLGDADAMLCGT
                     LGRFDQHLEHVRDIIGVEPGAPGYATLNALMLPDRTLFVTDTYVNEDPSAELLTAIAK
                     MAADEVSNFGLPPRLAFVSHSNFGSSHRPSALKVRRARDLFRDRHPGIMCDGEMHGDS
                     ALSATLRERAVADSTLQGEANLLVCPNLDAANILFNVLKITIGSGVTIGPILLGTAAP
                     AHVLTTSSTVRRVINMTALAVAEAQARRR"
     gene            9764..10675
                     /locus_tag="PK242_03800"
     CDS             9764..10675
                     /locus_tag="PK242_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOZ93172.1"
                     /translation="MSTLTELQTPKRVRSIGHAPARHWVGDGFPVHGLFGYSGAGVAE
                     RSPFLLLDYAAPTEFAPTTQRRGVGSHPHRGFETVTIVYAGEVEHRDSAGGGGVIGAG
                     DVQWMTAGGGIIHEEFHSAAYAARGGTFEVVQLWVNLPARDKLTPARYQAITEARIPV
                     VTLPDGAGRVRVIAGAHAGTRGPASTFSPMNVLDLRLAAGHTLALPQPEGWNTLVVVR
                     RGSLQVNGEAALRATEMATLSAAGRGLTLVAGDAADAELLVLAGQPIDEPVVGYGPFV
                     MNSREQIGQAIEDFNRGRFGRLGAGAG"
     gene            10845..11147
                     /locus_tag="PK242_03805"
     CDS             10845..11147
                     /locus_tag="PK242_03805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ93173.1"
                     /translation="MTTSPITLNESTRRFEQVVDGHTAYVEFERLPGQAIAYTHTVVP
                     EALGGRGIGSALVQHVMDYARQQGLQVRPDCSFIHAWIERHPAYQPLSLAHGATPR"
     gene            11246..11827
                     /locus_tag="PK242_03810"
     CDS             11246..11827
                     /locus_tag="PK242_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOZ93174.1"
                     /translation="MPVDPVALHGRRAALALLSAGLLAGCASRPLPRASTSATASDGA
                     VTRRAPVAPVPPTQTPLALDPAAREAAVAQALLLINTPYTYGGNTPQGGFDCSGLMQY
                     VFQQVGGAATRLPRSTAQWAAATLPVDEARLARGDLVFFNTTGAPFSHMGLYVGGEQF
                     VHAPSSGGTVRKDSLASRYFATRYQGARRVFAA"
     gene            complement(12247..13428)
                     /gene="metK"
                     /locus_tag="PK242_03815"
     CDS             complement(12247..13428)
                     /gene="metK"
                     /locus_tag="PK242_03815"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOZ93175.1"
                     /translation="MANSFLFTSESVSEGHPDKVADQISDAILDAILAQDERSRVAAE
                     TLTNTGLVVLAGEITTNAHVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSN
                     DIAQGVDHASDDHLNTGAGDQGLMFGYACDETDTLMPAPIYYAHRLVERQAQLRKSGA
                     LPFLRPDAKSQVTMRYVDGRPHSIETVVLSTQHSPDQSETATRMKASFIEAVVEEIIK
                     PVLPAEWLKDTRYLVNPTGRFVVGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDP
                     TKVDRSAAYAARYVAKNIVAAGLARQCQIQLAYAIGVAEPMNITIYTEGTGAIPDEQI
                     ARLVREHFDLRPKGIIQMLDLLRPIYQKTAAYGHFGRDEPEFTWERTDKAQALRAAAR
                     L"
     gene            13573..14406
                     /locus_tag="PK242_03820"
     CDS             13573..14406
                     /locus_tag="PK242_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOZ93176.1"
                     /translation="MLALYRLAARWPLSLLHALGAALGWLSWLASPTYRRRLRDNAAL
                     AGYGFAQVRPAVAHAGRMALETPRLWFGPPVPMRWDGTELVDAAYAAGRGVVFLTPHL
                     GCFEITAQGLAERYHARYGPITVLYRPARQAALAQVVETARRREGLDTAPTTLAGVRQ
                     MIKALRAGRAVGLLPDQVPPEGQGLWSPFFGQPAYTMTLAARLIQQTGAVPLLVWGER
                     RPRGQGYDLHFRALAEPLADELAPAVAQINREMERLIRECPTQYLWGYGRYKQPREQP
                     A"
     gene            14403..15281
                     /locus_tag="PK242_03825"
     CDS             14403..15281
                     /locus_tag="PK242_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HOZ93177.1"
                     /translation="MTTHPGVWLMRALAPLPLPWLRALGDVLGRLLFALARRRRHIAL
                     VNLRLAFPAWSEAERMDVARRSFIAFAQSFIDRAWVWHAPDAVVRQRIRLTGAVDELR
                     RPEAAVLFAPHFYGMDAGGAAIMQQAVAKGGSIYSPQSGAATDAWVRAGRERFGDVVL
                     INRRDGVKPVVKSLREGRYLYLLPDMDLGPEESVFVPFFGVPAATVPSLPRLARLGRA
                     RVVPTVTRMTPGGYEVQVHPAWSGYPGDDPVADTALMNRRLEDWIRTMPEQYYWVHKR
                     FKTRPAGEPSVYGRTP"
     gene            complement(15448..16362)
                     /locus_tag="PK242_03830"
     CDS             complement(15448..16362)
                     /locus_tag="PK242_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOZ93178.1"
                     /translation="MIETWLAELPHGITLSCRGAGARGRPLMLLLHGFPEAAFIWDAL
                     MAHFAQPEHGGFRCVAPNLRGFERSSAPAEVAAYKPALMVQDVLALAAAEGVDGRIHA
                     LVAHDWGGAFAWAAAHAHPAQVDHLVIINSPHPATFARELQRSPEQQQASAYMNFLAR
                     PDAEALLAEDDYRRLWPFFTSMGAGPERFGWLTEAVRQQYRDVWRHGLTGACNLYRVT
                     PLKPPAPGQRGVTDIPQPPPERVRVEVPTLVLWALDDTALRPGLLDGLDAHVPRLTVQ
                     RMPGATHWVVHEQPQQVAAHIGAFVTTK"
     gene            complement(16509..17219)
                     /gene="yihA"
                     /locus_tag="PK242_03835"
     CDS             complement(16509..17219)
                     /gene="yihA"
                     /locus_tag="PK242_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOZ93179.1"
                     /translation="MTARPAPAPAAAAARPDAATPAAAAREALGWLHTARFLTTAAQL
                     HQLPALAVPEIAFVGRSNAGKSTAINTLTQQTQLAFASRKPGRTQHINLFALGRQGGT
                     DAVLADLPGYGYAAVPLADKQRWQRVMANYLVTRENLAAVVMLCDPRHGLQELDEILL
                     EVIRPRVEQGLPFLILLTKADKLNRSEQARALQIARLQAGGGSVMLFSAPKRQGVEDA
                     ATWLRAVVDAAANPSDAA"
     gene            17342..17959
                     /locus_tag="PK242_03840"
     CDS             17342..17959
                     /locus_tag="PK242_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ93180.1"
                     /translation="MKSLAALLTASLLAATASAADAPAAPKVDLAKGAASYATVCVAC
                     HAADGNSTVPLQPSLAQQHPEYTLKQLLEFKSGARKDPVMQGFAAMLSEDDARNISWW
                     LAAQKAKPGAASDKDLVLTGERIFRGGIPDRHIPACAGCHSPNGAGIPSQYPRLSGQH
                     TDYTVKQMVAFRDGTRANNAVMQGVAAKMNDREIKAVSDYIAGLR"
     gene            18145..20271
                     /locus_tag="PK242_03845"
     CDS             18145..20271
                     /locus_tag="PK242_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOZ93181.1"
                     /translation="MTASHSTHGYQPRHGSAAWRASVELLSSMRFAIALLTVICIASV
                     IGTVIKQHEPAPNYVNQFGPFWAELFLKLQLNAVYSAWWFLLILAFLVVSTSLCVLRN
                     TPKFLADIRSYKENIRHQSLQAFHHKAEGELDESPEVAARRIGQQLAGGGWKVRLQAR
                     DTPEGQGWMVAAKAGAANKIGYIAAHSAIVLICLGGLFDGDLFVRAMTWLGGKSVYSG
                     GGLVSQVRPEHRLPETNPAFRGNLVVSEGTQSSTAILSQSDGVLLQELPFAVELKKFI
                     VEYYSTGMPKLFASEIVIHDKATGQTQPARVEVNHPASYKGVQIYQSSFDDGGSRVKL
                     RAVPMNGAAKAFEVEGTIGGNAQITNGSDKLTLEYTALRVINVENLSEGARGSGADVR
                     KVDLGQALTQRLGAADKTVTQRELRNIGPSITYKLRDAAGQANEFHNYMLPVDMGDGG
                     AVFLLGVRASQAEGFRYLRVPADEDGAIDGFVRLRGALQDAAQREQAVRRYVRKAVDP
                     QRPELVEQLAQSASRALGLFAGAEAVNGRTTGGLQAVSDFIETAVPESERERAAEVLL
                     RILNGSLLELNDLARQAQGKPPLTESEASRRFMTQAVLALSDAPLYPAPVAFELTGFE
                     QVQASVFQVARAPGKNVVYLGCLLLILGVFAMLYVRERRLWVWLAPRGAGAQASMALS
                     SNRKLLDVDREFEQWQRRLLRRESEL"
     gene            20268..>20616
                     /locus_tag="PK242_03850"
     CDS             20268..>20616
                     /locus_tag="PK242_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOZ93182.1"
                     /translation="MNTSVATANPTSVTLDGGFFARRTWGDWLFALLVLAGALFAFQR
                     YHASMDVYEKWILAGTAPVLIGLGWFWRPLRTLMLVVAGLSLLAVLSYAGDLRRADSV
                     FWLKYFLSSQSAIL"
BASE COUNT         3004 a         7133 c         7335 g         3144 t
ORIGIN      
        1 cggcgcggcc gtcggccacc cgttcggcgt ccaccagcac cgccaggtaa tcccagggcg
       61 ccagggtgaa ctcgggctgg cggtccagca gcagcagcac cgacgcatcc ggcaccaggc
      121 cgtcggtgtg ggtggcaaag gtggcgggcg tgacggcgcg aaacgccggc gcctcgcgca
      181 gcccctgcag gcaggcgcga aactccgccg cgtcggtgtg cggcggcgcc ggctgggcgt
      241 gggccagtgg caggtgggcc accaccacgg gcagcagcaa ggtggcgcac caagccagga
      301 aggtcgggac aggcatgaca gggtccggat gctgtgttct ccgtgattgt gaccgcgcgc
      361 tggccacact cggccagccg gaatagctgc cgcgccgcac caggaagggg tggggcgcac
      421 ggcgcaatca tgtgtattca gactgctggg tggccgtcgc gatccggtcg accatgctgc
      481 tcctgtgcag cacgcccatc ggccgccggc gtcacggggg ggcacccgac tcaggtcatg
      541 gattcaagta aaatcacgcc ccagggctta ctcaacaagc gccgtaagct atcattacgg
      601 tagcatccaa cacctctggt atctttcgtg gactgcttca tcaccggcac cgccggcttc
      661 atcggctttc acctggccaa actgctgctg gagcagggcc acaccgtgca cggctacgac
      721 ggcatcaccg actattacga cgtgcgcctc aagcacgccc gccacgatct gctgcgccaa
      781 tacccgcagt tcacggccac cgaggccatg ctggaagacg acgccaccct gcaggccgcc
      841 gtggccgccg cccagccccg cgccatcgtc cacctggccg cccaggccgg cgttcgctac
      901 agcatcgagc acccgcgcag ctacatcgac gccaacatca tcggcagctt caacgtcatg
      961 gaggccgcgc gccgccagca ggtggcgcac ctgctgatgg cctccacctc gtccgtctac
     1021 ggcgccaaca ccgagatgcc gtttgatgaa aaccagaagt gcgacatgcc aatgtcgttc
     1081 tacgccgcca ccaagaaggc caacgaggcc atggcgcaca gctgggccca catccacggc
     1141 gtgcccatca ccctgtttcg cttcttcacc gtgtacgggc cctggggccg gcccgacatg
     1201 gccctcttca aattcaccaa ggccatcctg gacggccagc ccatcgacgt ctacaaccac
     1261 ggcgacatgt ggcgcgactt cacctatgtg gaagacctgg tgcgcgccat cgccctgctg
     1321 atcgacgccg tgccgcccgc ggccgcaggc cgcaacccgg cacaggccat cgagggcgac
     1381 agcctctccc cggttgcgcc ctggcgcgtg gtcaacatcg gcaacagcga caaggtgcgc
     1441 ctgctggact tcatcgacgc catcgaacag tgcctgggcc aaaaggccat tcgcaaccac
     1501 ctgcccatcc aacccggcga cgtgcccgcc tcctgggccg atacccaatt gttgcagcgg
     1561 ttgaccggtt acaagccagc caccggcttt cagcaggggg tgcaggcgtt tgtgcgttgg
     1621 tactgcgagc accatcgccc gtagaccgac tctggcgcgc gccctggcct gtgtgtggtg
     1681 caggtgggcc gcgccgccgt gtcgaggtca gtctgtccga tcgctgggcc gatagccttc
     1741 caccaactgc tgcagcaggg cacggatgtc gtcgtgtcgc cgtgcccgca tgtgctcggc
     1801 cagctgctcg agccaggtct gcaactggtc ccagggcaga aagcgctcct gcgccttcag
     1861 gatgcggggg tgggcggtgc ggacgctggt gtcgtcgctc agcagctctt cgtacagctt
     1921 ttcgccggga cgcaggccgg tgacgcgaat ctcgattcct tctgcgtcgg gcgcgtcctg
     1981 cggccggcgt acccgctgac cggacagctc gatcatgcgg caggccaggt cgtggatgcg
     2041 caccggctcg cccatgtcca gcacgaacac ctcgcccccg caggccatgg cgccggcctg
     2101 gatgaccagc tgtgccgctt ccggaatggt catgaaatag cgcgtgatct cgcgatgcgt
     2161 gacggtcacc ggtccgccct gctcgatctg ttgccgaaac agtggcacca ccgagcctga
     2221 ggagcccagc acattgccga agcgcaccat ggtgaagcag gtgcgaccgc tgccaccggc
     2281 ccgctcgccc tgcgcatgca gcgcctgcaa caccatttcg gccagccgtt tgctggcgcc
     2341 catgacgctg gtggggcgca ccgccttgtc ggtgctgacc agcacgaaat gcccgacacc
     2401 atggcgctca gccgatgcgg cgcactccag cgtgccccag acgttgttgc gcagcccctc
     2461 gatcgggttg tgctcgacca gcggtacatg tttgtaggcg gcggcgtggt agacggtgtc
     2521 ggggtgccac tcggtcagaa tggcgtccag gcgcgccgca tcctgcaccg aacccagcag
     2581 cggtacgatg tgcagcgggc cgtcggcgtc gtccggctcg cccatcgccg ccagcagttc
     2641 gcggtggatc tcgtacaacg cgtattcgct ctgctccagc agcagcagca cgcgcgggcg
     2701 ccagaccagg atctggcggc acagctcgct gccgatggag ccgccggcac ccgtgaccat
     2761 caccgtgcgg ccgctcagct gctgctccac cagggcttcg tccgggcgaa ccgggtcgcg
     2821 acccagcagg tcttcgatgt ccaggtcctg cagatcgctg gcgtgcacgc cgcgccgcac
     2881 caggtcggcc atgccgggca aggtgcgcac gtgcaccgga tgggggcgaa gcagctcgac
     2941 gatctgcttg cgccgtgcca gactggccga ggggatggcc agcagcaccg acccgacctg
     3001 gtactcgcgc accagccgat ccagatcgga cggggcgaac accggcaggc catcgagccg
     3061 gcggccctgc agcgcggggt tgtcgtccag atagccaacc aggcgctgct ggtgcatgcg
     3121 caacaggctg gcggccagtt gcaggcccga ctggccggcg ccatagatca gcacgccagg
     3181 ggcgttgagt cgggccagca gctcgctgta caggtcgccg atccacagcc gcacggtgac
     3241 ccggctcagg cccagggcga cgaacatcag cagcggctgg atgatgccta tcgagcgcgg
     3301 gataccgggc accgcgatca gcgtgaacac cagcgcgtac agccccccgt acaccgcgac
     3361 cgccttgccc agcgccagcg tggcgttcca gccggcgtag cgaaagatcg aggcatacag
     3421 cccgaagtga gcaaacaccg gcaggcccag cagccacgac ccgagcgtgg cccaggcatg
     3481 catgcgaccg aacggcaccc agtgctccag ccgcagcgcc caggccagcc agacggtgaa
     3541 ggcgcacaga aaggcgtcca gcgtcagggc cagcagcctc ttgccggcac gcggcatgcg
     3601 gatcagtcgg tgggtcaggc cctgcaagct catgccagcg ttcgcgcgtg cggaagtgcc
     3661 aggtgggggc ccgctgaccg gcggggtgca ggagttcggg cgagccgtcc agccgtcatg
     3721 cgggtttcga gggatgcgca ggcccagggt gagtcgatcg aatgccagcc cgatcgggct
     3781 ggcgtgcgcg atcttacccc ccgcgtgcgc ggcgtcgacg gcgcagggcc ggagcgcgcg
     3841 cagtcgatgg ctgccgcttc atgcggggtg cggtcgaatg gctggcgcct gatccagcgc
     3901 cgatgggcat ggcgccgtcc cgtgaacttc aaaatccata gctgctggcg ctcgccagac
     3961 gggcgccaga ggccagaaat ccttgaaacc tacagatcag gcggcggcac tgggcgcctg
     4021 cagctccagc gtcaggcgca gggtgtcggg ctcgcccggg gtgggcgctt cggcaaagcc
     4081 cagcgcgcgc gccagctcgc gcatgcgcca gttctcgacc agggcgctgg cgatcaggcg
     4141 ctgggtgccg gcgccgcgct ggtagcggat cagtttgtcc atcagcagcc ggcccaggcc
     4201 ggagcccttg agttccggcc gcaggatgat gccgaattcg gcgccgaggt tgtccgggtc
     4261 ggccagcgcg cgcgccacgc ccagggtctg ctcgccgcct tcgggcgcgg gcagggtggc
     4321 gatgaaggcc atctcgcgcg tgtagtcgat ctgcaccagc cgcgccagct cgctgcgctc
     4381 catggtgcgg cggctgtaga acaggcgcat gcggacgtcc tcggcgtcca ggctttccag
     4441 gaaggcgcgg tgctgcagct cgtcttcggg gcggatcggg cgcagcgtca ggcgctggcc
     4501 tttccattcc agggtctcga tcagttgcgc cgggtagggc gcgatggcaa agtgcgccgc
     4561 gccggccggc aggcgcgggc tgacgcgcac gcgcgcatcc agcgcaatgg cgccttcgtg
     4621 gttgacgatc agtgggttga tgtcgagctc ggccacctcg ggcacgtcga ccagcagctg
     4681 cgacaccgcc atcagggtgg cgaccacggc gtccaggtcg gccgccggca cgtcgcggta
     4741 gccggccagc aggcgcgcca cgcgggtgcg cgcgatcagc gcgcgcgcca gcgggtcgtt
     4801 cagcggcggc agcgccatgg cgctgtcctt catcacctcg accgccgtgc cgccctggcc
     4861 gaacaggatc accgggccga agctggggtc gatgctggcg ccgatgatca gctcctgcgc
     4921 ctgcttgcgc cgcaccatgg cctgcacgct gaagccgtcc agccgcgcct tcggctggct
     4981 cttgcggatg cgcgccagca tggcgctggc ggcggcgcgc acgtcggctt cgtcctgcag
     5041 gttgagcacc acgccgccga cgtcggattt gtgcgagatg tcttccgaca ggatcttgat
     5101 gaccaccgga aagccgatgc gcgccgcttc ggcggccgcc gcctcggggt cggccggcgt
     5161 ggtgcgcgtg gccaccaccg ggatgtggta ggcctcgcac accgccttgg cctcgggttc
     5221 ggtcagcatg tcgcggccgc tggccagcac cgcctgcacc agggcgcgca cgcgtgccac
     5281 gtcgggcgtg ctgccgttgt gcagctgctc ggccggcggg gcctcggcca gctcggcctg
     5341 gttgcgggcg taggcctgca gcatggcgat ggcggccacg gcctgctcgg gcatgtcgta
     5401 gctggcgatg ccggcttcga tgaacagctg gcgcgcttcg gccaccgccg ggccgccgac
     5461 ccagcagctg accagcggca gcggcgcgcc accttccggc cgcagcgccg gcagcagggc
     5521 acgcgcgatg tcggtcgagg gcacgatggc ggtgggcgcc tggatgaaca gcaccgtgcc
     5581 gacttcctgc gggtggcgcg tcagtgcctg cagcgcgtcg acgtagcgct gcgccggcgc
     5641 gtcgccgatg atgtccaccg ggttgccgcg cgaccagttg gccggcaaca ccgcgtccag
     5701 tgcgccgacc agggccgggc tcagttccgc cagtggcacg tcgtgggcgg cggcggcgtc
     5761 ggccgccagc acgcctaccc cgccgccgtt ggtcaggatg gtcatgcgat cgccgatcgg
     5821 ggcggtctgg cgcgtcagga tctcggccgc caggaacagc tcttccagac tgtccacccg
     5881 cagcatgccg gcgcgggcaa tcgccgcgtc caccacgtcg tcggcgccgg ccagcgcgcc
     5941 ggtgtgcgag gccgcggcct tctggccctg ggcgctgcgc ccggccttga tgacgatgac
     6001 cggcttgttg cgcgccgccg cgcgcgccgc cgacatgaac ttgcgtgcct ggtcgatcga
     6061 ctcgatgtac agcaggatgg cgcgtgtgcg ggcgtcgctg gccaggtagt ccagcaggtc
     6121 accgaagtcg acgtcggcgt gttcgcccag cgacacgaag tgcgaaaagc cgatctgtcg
     6181 cgcttcggcc cagtccagca tggcggtgac cagggcgccc gactgcgaga cgaaggccag
     6241 ctcgcccggc cgcgccggga tgtgggcgaa gctggcgttc agccgcgcgt gcggcgccag
     6301 caggccgacg cagttggggc ccagcacgcg cagcaggctg gggcgggcgg cgtccagcat
     6361 ggcctgcttc tgctcggccg tgaggccggc gctgatgacc accgccgccc gcgtgccacg
     6421 cgcggccagc tggccgatca gctcgaccac cgtgtgggcg ggggtgcaga tcaccgccag
     6481 ctcgggcgct tcgggcagat cgtccacgct ggcgtagcag cgctggcccg acagcgtgcg
     6541 gtgcttgggg ttgaccggat agaccgggcc ggcaaagccg ccgtcgtgca tgttgcgcca
     6601 cagcgtgccg ccgacggaaa agggtcgctc ggaggcgccg atgacggcca ccgactgagg
     6661 gtcgaacagg gaatcgaggt tgcggatgct catgacttgt cagaactcgg aaccgcacgg
     6721 ccgggcgcgt gcgtgggaag gcggccgatg gccggggcca gggtgcagcg tgtgcacaac
     6781 ccttgatcct accccgtggt ccgggccggg cttgctgccc ctgcaccacg cagtcaccgg
     6841 accaggacct gccgtccaaa cgcgccgccg cggcgaacgc catgtcagcg caaaaaaggt
     6901 cgtcagcgct tatctggcaa gcgccggcag ctacattaaa gatagcaatt cgagaatgac
     6961 ccatggccaa tgacctcgct gcgcttggtt gacagatggg tcatcggtca tgcagggcgc
     7021 agcccgcctc atgggccact cgccatcggt catcggtggt ggagcgcacg ccccaccgcc
     7081 ccaggtcagc gtcggcgcgc ctgcgcctcg gccaccgcca gcgccgtcat gttgatgacc
     7141 cgccgcacgg tggatgaggt ggtcagcacg tgcgccggcg ccgccgtgcc cagcaggatg
     7201 gggccgatgg tgacgccgct gccgatggtg atcttgagca cgttgaacag gatgttggcg
     7261 gcgtccaggt tggggcagac cagcaggttg gcctcgccct gcagcgtgct gtcggcgacg
     7321 gcgcgctcgc gcagcgtggc cgacagggcg ctgtcgccgt gcatctcgcc gtcgcacatg
     7381 atgcccgggt gccggtcgcg gaacaggtcg cgcgcgcgtc gcaccttcag cgccgacgga
     7441 cggtgcgagg agccgaagtt ggagtgcgac acgaaggcca gccggggcgg caggccgaag
     7501 ttgctgactt catcggccgc catcttggcg atcgccgtca gcagctcggc gctggggtct
     7561 tcgttgacgt aggtgtcggt gacgaacagc gtgcggtcgg gcagcatcag cgcgttcagc
     7621 gtggcgtagc ccggggcgcc gggttcgacg ccgatgatgt cgcgcacgtg ctcgaggtgc
     7681 tggtcgaagc gccccagcgt gccgcacagc atggcgtcgg cgtcgcccag gtgcaccatc
     7741 agcgcggcga tgatggtggt ggagcggcgc acggcggcct tggccgcgtc caccgtgacg
     7801 ccgccgcgcg ccatcaggcc gtgataggtc tcccaatatt ggcgaaagcg ccggtcgtcc
     7861 tcggggttga cgcattccac gtcctggccc aggcgcatgc gcaggccggc cttggcgatg
     7921 cgctgctcga tcaccgccgg gcggccgatc aggatcggcg tggcgatgcc gtcgtccagc
     7981 acgatctgcg ccgcgcgcag cacgcgctcg tcctcgccct cggcataggc cacgcgcttg
     8041 gcctcgggcg ccaccgcctt ggcggcggcg tagaccgggc gcatcaccat gccggtctgg
     8101 tagacgaagc gctccaggct ggcgcggtag gccgccatgt cgctgatcgg ccggctggcc
     8161 acgcccgact cgaacgccgc ccgtgccacc gccggcgcga tgcgcatgat caggcgcggg
     8221 tcgaacggcg tggggatgat gtagtcgggg ccgaactgca ggtccttgcc ggcgtaggcg
     8281 gcggccacct cggcgctggt ttcggccttg gccagggcgg cgatttcgtg cacgcaggcc
     8341 agcttcattt cctcggtgat cttgctggcg ccgcagtcca gcgcgccgcg gaagatgtag
     8401 ggaaagcaca ggacgttgtt gacctggttg gggtagtccg agcgtccggt ggcgatgatg
     8461 cagtcgggcc gcgcggcctt ggcgtcttcg gggcggattt cgggttcggg gttggccagc
     8521 gccaggatca cgggcttggc ggccatggtc ttgaccatct cgcccgtcag cacgccgggg
     8581 gccgagcagc ccagaaacac gtcggcgccc ttgaccacgt cggccagcgt gcgcgcgccg
     8641 gtgtcgggct gggcgtagcg cgccttcgac tcatcgaaac cgcccgcgcg gccgtggtag
     8701 atcaggccct tggagtcgca ggcgaacaca tgcgcgggct tcacgcccag cgccaccatc
     8761 acgtccaggc aggcgatggc ggcggcaccg gcgccgctga cggccacctt cacgtcgccg
     8821 atctgcttgc ccaccagctc cagcccgttg agcagggcgg cggccgagat gatggcggtg
     8881 ccgtgctggt cgtcatggaa caccgggatg ttcatgcgct cgcgcagctt gcgctcgatg
     8941 tagaagcact cgggcgcctt gatgtcttcc aggttgatgc cgcccagcgt cggctccagg
     9001 ctggcgatga tctcgaccag cttgtcgggg tcgcgctcgg ccagctcgat gtcgaacacg
     9061 tcgatgccgg cgaacttctt gaacaggcag cccttgccct ccatcaccgg cttgccggcc
     9121 agcgggccga tgtcgcccag gcccagcacg gcggtgccgt tggtcaccac gcccaccagg
     9181 ttgccgcggc tggtgaattc ggccgccttg gacgggtcgg cctgaatgtc caggcagggg
     9241 taggccacgc cgggcgagta ggccagcgac agatcgagct ggttggacag cggcttgagg
     9301 ggggtgaccg cgatcttgcc gggtttgggg ttgcggtggt attcgcgcgc agcgtcgcgc
     9361 agggcggctt cggcgggggt gagcggcttg ttcatgcggt ctccagtgcg ttgtcttcgt
     9421 ggccgggaac gtgagcctac atcagttcat ccgcgttgcc gaggtgggct ggggccgcag
     9481 aggcgccaat cgtctgccgc cggcatggcg ccgtggttgg gtaaaaaaac gggctgcagc
     9541 gcttgatggt caagcgccag cagctatcaa aatcagagta tctgtggtgc ggaccgcggt
     9601 ggccggggcg ggcgcgggtg gccacggcga ttgtttcgat tctggaacgg tgtggcggca
     9661 ccgcgcccct tggtggccgg ccgctgcgcc cctaccatcg gagcatgggc gtcgaccgtg
     9721 gggtcggcgc cgcgggccga ccggccctca ccggagcatc accatgtcca ccctcaccga
     9781 gcttcagaca cccaagcgcg tgcggtccat cggtcacgcg cccgcccgcc actgggtggg
     9841 cgacggcttt ccggtgcacg gcctgttcgg ctacagcggt gcgggcgtgg ccgagcgcag
     9901 cccctttctg ctgctggact acgccgcgcc caccgaattc gcacccacca cgcagcggcg
     9961 cggcgtcggc agccacccgc accgcggctt cgagaccgtg accatcgtct acgcgggcga
    10021 ggtcgagcac cgcgactcgg ccggcggcgg cggcgtgatc ggcgccggcg acgtgcagtg
    10081 gatgaccgcc ggcggcggca tcatccacga ggagttccac tcggccgcct acgccgcgcg
    10141 cggcgggacg ttcgaagtgg tgcagctgtg ggtcaacctg ccggcgcgcg acaagctgac
    10201 gccggcgcgc taccaggcca tcaccgaggc ccggattccg gtcgtgaccc tgcccgacgg
    10261 cgccggtcgc gtgcgcgtga tcgccggcgc ccatgccggc acccgcgggc cggccagcac
    10321 cttcagcccg atgaacgtgc tggacctgcg cctggccgcc ggtcacacac tggcgctgcc
    10381 ccagcccgag ggctggaaca cgctggtggt ggtgcgccgc ggcagcctgc aggtcaacgg
    10441 cgaggcggcc ctgcgcgcca ccgagatggc cacactgtcg gccgccggcc gcggcctgac
    10501 gctggtggcc ggcgacgccg ccgacgccga actgctggtg ctggccggcc agccgatcga
    10561 cgagccggtg gtcggctacg gccccttcgt gatgaacagc cgcgagcaga tcggccaggc
    10621 catcgaagac ttcaaccgcg ggcgctttgg ccgcctgggg gccggggcgg gctgaaaccg
    10681 gcgattgcac accggacatc cgtacgcaga agacgcagaa caaacctgaa tgcgccttgg
    10741 cgtcctctgc gcaatctctg ggtcctctgc gtatggatgt tccggaatgc ccaccaatgc
    10801 agaacgcgac accctgccgc cccaacattc actgaacgca aacaatgacc acctccccca
    10861 tcaccctgaa cgaatccacc cgccgcttcg agcaggtggt ggacggccac accgcctacg
    10921 tcgagttcga gcgcctgccc ggccaggcca tcgcctacac ccacaccgtc gtgcccgagg
    10981 cgctgggcgg gcgcggcatc ggcagcgcgc tggtgcagca cgtgatggac tacgcccgcc
    11041 agcagggcct gcaggtgcgg cccgactgca gcttcatcca cgcctggatc gagcgccacc
    11101 ccgcctacca gccgctgtcg ctggcgcacg gcgccacgcc gcgctgactg gaccagacca
    11161 gaccgggccg gcgcgctgga cagggttcgc ctgctattca taaaatagct gctcgcgctt
    11221 tctggacaag cgccggagcc ctgaaatgcc tgtagacccg gttgccctgc atggccgccg
    11281 tgcggcgctg gccctgctgt cggccggcct gctggccggc tgcgccagcc ggccgctgcc
    11341 acgcgccagc accagcgcca ccgccagcga cggcgcggtg acccggcgcg cgcccgtcgc
    11401 cccggtgccg cccacgcaga cgccgctggc gctcgacccg gccgcgcgcg aagccgccgt
    11461 ggcgcaggcg ctgctgctca tcaacacccc ctacacctat ggcggcaaca cgccgcaggg
    11521 cggcttcgat tgcagcggtc tgatgcagta tgtgttccag caggtcggcg gcgccgccac
    11581 acgcctgccg cgcagcaccg cgcaatgggc ggcggccacg ctgccggtgg acgaagcgcg
    11641 gctggcgcgc ggcgacctgg tgttcttcaa caccaccggc gcgccgtttt cgcacatggg
    11701 gttgtacgtg ggcggcgagc agttcgtgca cgcgccttcg agcggcggca cggtgcgcaa
    11761 ggacagcctg gccagccgct acttcgccac ccgctaccag ggcgcgcggc gggtgtttgc
    11821 ggcatgaacc tagaaacggc agttgcccgc ttgccaacct cgggcagacc gcatcgatgc
    11881 ggggtctggc ggctgcgatg gccttcacct gttgggtgag cgcgctgcgc acccacccgc
    11941 accgcgctcg ctgcgccatg cggcgttgct cctgcttgcg ggcaccagcc cgcggcggcg
    12001 tcgcgtcttg cctgccacgc cgatcacggc acgggcgggt acgtccaacg gccgtttcta
    12061 ggttgaaatg ggcggtgagg gacacccgag acgggaggcg atgcaaggcg caaagcgcag
    12121 ccacagcccg tgccgtggcc agcatgggca gcgccgcagc gtgcccgtat cgggcggacg
    12181 gcgccgatcc ggtttcagtc tttcagtgga cccataggat gaaaaaagcc gacgggcgcc
    12241 gtgcgcttac agcctggccg ctgcacgcag cgcttgggct ttgtcagttc tttcccaggt
    12301 gaactcgggc tcgtcgcggc cgaagtggcc gtaggcggcg gtcttctggt agatcgggcg
    12361 cagcaggtcc agcatctgga tgatgccctt ggggcgcagg tcgaagtgct cgcgcaccag
    12421 cctggcgatc tgctcgtccg gaatggcgcc cgtgccttcg gtgtagatgg tgatgttcat
    12481 cggctcggcc acgccgatgg cgtaggccag ctggatctgg cactggcgcg ccagcccggc
    12541 ggcgacgatg ttcttggcca cgtagcgggc ggcgtaggcg gcgctgcggt cgaccttggt
    12601 cggatccttg ccgctgaagg cgccgccgcc gtgcgggcag gcgccgccgt aggtgtcgac
    12661 gatgatcttg cgcccggtca ggccgcagtc gccctgcggg ccgccgacca cgaagcggcc
    12721 ggtggggttg accaggtagc gcgtgtcctt gagccactcg gcgggcagca cgggcttgat
    12781 gatttcctcg accacggctt cgatgaagct ggccttcatc ctggtggcgg tctcgctctg
    12841 gtcggggctg tgctgggtgg acagcaccac ggtctcgatg ctgtgcggcc ggccatcgac
    12901 gtagcgcatg gtcacctggc tcttggcgtc ggggcgcaga aagggcaggg cgccgctctt
    12961 gcgcagctgc gcctggcgct cgaccaggcg gtgcgcgtag tagatgggcg ccggcatcag
    13021 ggtgtcggtc tcgtcgcagg cgtagccgaa catcaggccc tggtcgccgg cgccggtgtt
    13081 caggtggtcg tcgctcgcgt ggtccacgcc ctgggcgatg tcgttgctct gcttgtcgta
    13141 ggccaccagc accgcgcagc ccttgtagtc gatgccgtat tcggtgttgt cgtagccgat
    13201 gcgcttgatg gtgtcgcgcg ccacctggat gtagtccaca tgggcgttgg tggtgatttc
    13261 gccggccagc accaccaggc cggtgttggt cagcgtttcg gcggccacgc gcgagcgctc
    13321 gtcctgggcc aggatggcgt cgaggatggc gtcggagatc tggtcggcga ccttgtcggg
    13381 gtggccctcg gaaaccgatt cggaggtgaa gagaaagctg ttcgccagtt ccatttgaat
    13441 aaactccacg ggtttgaacg ttgcgttgcc aaactgtcgg gagccctggc gaacgcttta
    13501 gcagcacttg tttaacgtcg ccctgcaagt agttccataa ctcggcgacg gcgcgatttt
    13561 actggatcca ccatgcttgc cctgtaccgc ctggcggcgc gctggccgct gtcgctgctg
    13621 cacgcgctgg gcgcggcgct gggctggctg agctggctgg cgtcgcccac ctaccggcgc
    13681 cggctgcgcg acaacgcggc gctggccggc tacggctttg cccaggtgcg cccggcggtg
    13741 gcgcacgccg gccgcatggc gctggagacg ccgcgcctgt ggttcggccc gccggtgccc
    13801 atgcgttggg acggcaccga actggtggac gccgcctacg ccgccggccg tggcgtggtg
    13861 ttcctgacgc cgcacttggg ctgcttcgag atcaccgccc agggcctggc cgagcgctac
    13921 cacgcgcgct acggccccat caccgtgctg taccgtccgg cgcgccaggc ggcgctggcg
    13981 caggtggtgg agacggcgcg ccggcgcgag gggctggaca ccgcgcccac caccctggcc
    14041 ggcgtgcgcc agatgatcaa ggcgctgcgc gccgggcgcg ccgtcggcct gctgcccgac
    14101 caggtgccgc ccgaggggca gggtctgtgg agcccgtttt ttggccagcc ggcctacacc
    14161 atgacgctgg cggcgcggct gatccagcag accggcgccg tgccgctgct ggtgtggggc
    14221 gaacggcggc cgcgcgggca gggctacgac ctgcacttcc gcgccctggc cgagccgctg
    14281 gcggacgagc tggcgcccgc ggtggcgcag atcaaccgcg agatggagcg cctgatccgg
    14341 gaatgcccga cgcagtacct gtggggctat ggccgctaca agcaaccgcg ggagcagccg
    14401 gcgtgacgac ccaccccggc gtctggctga tgcgcgcgct ggcgccgctg ccgctgccct
    14461 ggctgcgcgc gctgggcgac gtgctggggc ggctgctgtt cgcactggcg cggcggcggc
    14521 gccacatcgc gctggtcaac ctgcggctgg cctttcccgc ctggagcgag gccgagcgca
    14581 tggacgtggc gcgccgcagc ttcatcgcct ttgcccagtc cttcatcgac cgcgcctggg
    14641 tctggcacgc gcccgacgcc gtggtgcgcc agcgcatccg cctgaccggc gcggtggacg
    14701 agctgcgccg gcccgaggcg gcggtgctgt ttgcgccgca cttctacggc atggacgccg
    14761 gcggcgcggc catcatgcag caggcggtgg ccaagggcgg cagcatctac agcccgcagt
    14821 cgggcgccgc caccgacgcc tgggtgcgcg ccgggcgcga gcgcttcggc gacgtggtgc
    14881 tgatcaaccg gcgcgacggt gtcaagccgg tcgtcaagtc gctgcgcgag ggccgctacc
    14941 tgtacctgct gcccgacatg gacctggggc cggaggaatc cgtcttcgtg cccttcttcg
    15001 gcgtgccggc ggccaccgtg ccgtcgctgc cgcggctggc gcggctgggg cgcgcgcgcg
    15061 tggtgcccac cgtcacccgc atgacgcccg gcggctacga ggtgcaggtg cacccggcct
    15121 ggagcggcta cccgggcgac gacccggtgg ccgacaccgc gctgatgaac cgccggctgg
    15181 aagactggat ccgcaccatg cccgagcagt actactgggt gcacaagcgc ttcaagacgc
    15241 ggccggcggg cgagccgtcg gtgtatggac gcaccccctg agtcgccttc ggcgccatct
    15301 cccggagggg gacaacgcca gtggccggcg caggtccggc cacggcgttc gctggcctgg
    15361 cctgctccgc ggtcgttgga tggcgatggg cgaatgtcct acgccaaata ggcatttggc
    15421 gcgcttccag gctgcgcgag cagctatcta tttcgtagta acaaaggcgc cgatgtgcgc
    15481 ggccacttgc tgcggctgct cgtgcaccac ccagtgcgtg gcgccgggca tccgttgcac
    15541 ggtcaggcgc ggcacgtggg cgtccaggcc gtccagcagg ccggggcgca gcgcggtgtc
    15601 gtccagcgcc cacagcacca gggtcggcac ctccacgcgc acgcgctcgg gcggcggctg
    15661 cgggatgtcg gtgacgccgc gctgccccgg cgccgggggc ttgagtggtg tgacgcggta
    15721 caggttgcag gcgccggtca ggccatggcg ccagacgtcg cggtactgct ggcgcacggc
    15781 ctcggtcagc cagccgaagc gctccgggcc cgcgcccatc gaggtgaaga acggccacag
    15841 gcggcggtag tcgtcctcgg ccagcagcgc ctcggcgtcg ggccgggcca ggaagttcat
    15901 gtaggcgctg gcctgctgct gctccgggct gcgctgcaac tcgcgcgcga aggtggcggg
    15961 gtggggcgag ttgatgatca ccaggtggtc gacctgggcc gggtgcgcat gcgccgccgc
    16021 ccaggcaaag gcgccccccc agtcgtgcgc caccagggcg tggatgcggc cgtccacccc
    16081 ctcggcggcg gccagcgcca gcacgtcctg caccatcagc gccggcttgt aggcggccac
    16141 ctccgccggc gcgctggacc gctcgaagcc gcgcaggttg ggcgcgacgc agcgaaagcc
    16201 gccgtgttcg ggctgcgcga aatgcgccat cagcgcatcc cagatgaagg cggcctctgg
    16261 aaagccgtgc agcaacagca tcagcggccg cccgcgtgcg ccggcgccgc ggcagctcag
    16321 ggtgatgccg tggggcagtt cggcgagcca ggtttcgatc atcttgagcg ctccttgttt
    16381 gatagctgct ggcgcgcgcc aatcatgcgt tggtgccggt tttgcccgaa tgtcggcgcc
    16441 atggtagagc gcgcggtgcc ggtggcgcaa ggctcggccg tccgaccggt gacggcgcag
    16501 ccgtcgcctc aggcggcgtc ggatgggttg gcggcggcgt ccaccaccgc gcgcagccat
    16561 gtcgcggcgt cttccacgcc ctggcgcttg ggggcggaaa acagcatcac gctgccgccg
    16621 ccggcctgca ggcgggcgat ctgcagcgcc ctggcctgtt cgctgcggtt gagcttgtcg
    16681 gccttggtca gcaggatcaa aaacggcagg ccctgctcga cgcgcgggcg gatcacttcg
    16741 agcaggattt cgtcgagctc ctgcagcccg tggcgcgggt cgcacagcat gaccacggcg
    16801 gccaggttct cgcgcgtcac caggtagttg gccatcacgc gctgccagcg ctgcttgtcg
    16861 gccagcggca cggcggcgta gccgtagccc ggcagatcgg ccagcacggc gtcggtgccg
    16921 ccctggcggc ccaacgcgaa caggttgatg tgctgggtgc gccccggctt gcgcgaggcg
    16981 aaggccagct gggtctgctg cgtcagggtg ttgatggcgg tcgacttgcc ggcgttggag
    17041 cggccgacga aggcgatttc cggcacggcc agcgccggca gttggtgcag ctgggcggcg
    17101 gtggtcagaa agcgcgccgt gtgcagccag cccagcgcct cgcgcgccgc cgcggctggg
    17161 gtggcagcat cggggcgggc ggcggcggcg gcgggggctg gggcagggcg ggcggtcatg
    17221 cgaggggccg cgccatgtgg gcgccggggc ggcctggatc aaggaatggc gggtgggggc
    17281 attgtagaat cgcccggatt cacgccccgt gacaacccct tcccacaaga cgatgcgtcc
    17341 catgaaatcg cttgccgccc tgcttaccgc ctccctgctg gccgccaccg cctcggcggc
    17401 cgacgcaccc gcggcgccca aggtcgatct ggccaagggc gcggccagtt acgccaccgt
    17461 gtgcgtggcc tgccatgcgg ccgacggcaa ttccaccgtg ccgctgcagc ccagcctggc
    17521 ccagcagcac ccggaataca ccctcaagca gctgctggaa ttcaagagcg gcgcgcgcaa
    17581 ggacccggtg atgcagggct ttgccgccat gctgagcgag gacgacgcgc gcaacatttc
    17641 gtggtggctg gccgcgcaga aggccaagcc cggcgccgcc agcgacaagg acctggtgct
    17701 gaccggcgag cgcatcttcc gcggcggcat tccggaccgc cacatcccgg cctgcgccgg
    17761 ctgccacagc cccaatggcg ccggcatccc gtcgcaatac ccgcgcctgt cggggcagca
    17821 caccgactac acggtcaagc agatggtggc cttccgcgac ggcacgcgcg ccaacaacgc
    17881 cgtcatgcag ggcgtggccg ccaagatgaa cgaccgcgag atcaaggccg tgtccgacta
    17941 catcgccggc ctgcgctgag cgccggcccg gcctgcccgt gcgccaagcc gcgtccatgc
    18001 cccgggcgcg gcttttgttt tcctggccgc cgatcgtgac ccaagtcaag ataatcaccg
    18061 tgtcggtgga acccggcgcc ccagatcgac tccagccctg atggcccgac gccggacggc
    18121 atggccgccg ccgccacccc ttgcatgacc gcctcccaca gcacccacgg ctaccaaccc
    18181 cgacatggct cggccgcctg gcgcgccagc gtcgagttgc tgtcgtcgat gcgctttgcc
    18241 atcgcgctgc tcaccgtcat ctgcatcgcc tcggtgatcg gcaccgtcat caagcagcac
    18301 gagccggcgc ccaactacgt caaccagttc gggccgttct gggccgagct gttcctcaag
    18361 ctccagctca acgccgtcta cagcgcctgg tggttcctgc tgatcctggc ctttctggtc
    18421 gtcagcacca gcctgtgcgt gctgcgcaac acgcccaagt ttctggccga cattcgcagc
    18481 tacaaggaaa acatccgcca ccagagcctg caggcctttc accacaaggc cgagggcgag
    18541 ctggacgaaa gccccgaggt ggcggcgcgg cgcatcggcc agcaactggc cggcggcggt
    18601 tggaaggtgc gcctgcaggc gcgcgacacg cccgaaggcc agggctggat ggtggcggcc
    18661 aaggccggcg ccgccaacaa gatcggctac atcgcggcgc acagcgccat cgtgctgatc
    18721 tgcctgggcg ggctgttcga cggcgacctg ttcgtgcgcg ccatgacctg gctgggcggc
    18781 aagagcgtct acagcggcgg cggcctggtg tcccaggtgc ggcccgaaca ccgcctgccc
    18841 gaaaccaacc cggcgttccg cggcaacctg gtggtgtcgg agggcacgca gtcgagcacc
    18901 gccatcctca gccagtccga cggcgtgctg ctgcaggagc tgccgttcgc ggtcgagctg
    18961 aagaagttca tcgtcgagta ctactccacc ggcatgccca agctgttcgc cagcgagatc
    19021 gtgatccacg acaaggccac cggccagacg cagccggcgc gcgtcgaggt caaccacccg
    19081 gccagctaca agggcgtgca gatctaccag agcagcttcg acgacggcgg ctcgcgcgtc
    19141 aagctgcgcg ccgtgcccat gaacggcgcg gccaaggcgt tcgaggtcga gggcaccatc
    19201 ggtggcaacg cccagatcac caacggcagc gacaagctga cgctggaata cacggcgctg
    19261 cgcgtgatca acgtcgaaaa cctcagcgaa ggcgccaggg gctcgggcgc cgacgtgcgc
    19321 aaggtcgacc tgggtcaggc gctgacgcag cggctgggcg cggccgacaa gacggtgacg
    19381 cagcgcgagc tgcgcaacat cggccccagc atcacctaca agctgcgcga cgcggccggc
    19441 caggccaacg aattccacaa ctacatgctg ccggtggaca tgggcgacgg tggcgcggtg
    19501 ttcctgctgg gcgtgcgcgc cagccaggcc gagggctttc gctacctgcg cgtgccggcc
    19561 gacgaggacg gcgccatcga tggcttcgtg cgcctgcgcg gcgcgctgca ggacgcggcc
    19621 cagcgcgagc aggcggtgcg ccgctacgtg cgcaaggcgg tcgacccgca gcgccccgaa
    19681 ctggtggagc agctggcgca gtcggccagc cgcgcgctgg ggctgtttgc cggcgccgaa
    19741 gccgtcaatg gccgcaccac cggcggtctg caggcggtgt cggacttcat cgagacggcg
    19801 gtgcccgaaa gcgagcgcga acgcgccgcc gaggtgctgc tgcgcatcct caacggcagc
    19861 ctgctggagc tcaacgacct ggcgcgccag gcccagggca agccgccgct gaccgagtcc
    19921 gaagccagcc ggcgcttcat gacgcaggcg gtgctggcgc tgtcggacgc gccgctgtac
    19981 ccggcgccgg tggcgttcga gctgaccggt ttcgagcagg tgcaggccag cgtgttccag
    20041 gtggcgcgag ccccaggcaa gaatgtggtc tatctgggct gcctgttgct gatcctgggc
    20101 gttttcgcca tgctgtatgt gcgcgagcga cgcctgtggg tgtggctggc gccgcgcggc
    20161 gcgggcgcgc aggccagcat ggcgctgtcg tccaaccgca agctgctgga cgtcgaccgc
    20221 gagtttgaac agtggcagcg caggctgctg cgcagggagt ccgagctatg aacacttccg
    20281 tggccaccgc caaccccacc agcgtcaccc tggacggggg ctttttcgcg cgccgcacct
    20341 ggggcgactg gctgttcgcg ctgctggtgc tggcgggcgc gctgttcgcg ttccagcgct
    20401 accacgcgtc gatggacgtg tacgaaaagt ggattctggc cggcaccgcc ccggtgctga
    20461 tcggcctggg ctggttctgg cgcccgctgc gcacgctgat gctggtggtg gcggggctgt
    20521 cgctgctggc cgtgctttcg tatgcggggg atctgcggcg tgccgattcg gtgttctggc
    20581 tcaagtactt cctgtccagc cagtcggcca tcctgt
//
LOCUS       DAOOOG010000028        20570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_2545_length_20570_cov_23.362856, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000028 DAOOOG010000000
VERSION     DAOOOG010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 20570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20570
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2545_length_20570_cov_23.362856"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..593)
                     /locus_tag="PK242_03855"
     CDS             complement(159..593)
                     /locus_tag="PK242_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93183.1"
                     /translation="MPISAPTASRLRVARRCGLLAVAALALAGCAALTPRTPEEIVAQ
                     RVEERWDALIKGDFPKAWTYTQPAYRAIVKQADYRRTFGFAGQWRGVQVHKVSCEAER
                     CKARIRLTTRVLTPPFQGQEVAGVIDETWVREDGNWWYYQNF"
     gene            complement(658..3138)
                     /gene="gspD"
                     /locus_tag="PK242_03860"
     CDS             complement(658..3138)
                     /gene="gspD"
                     /locus_tag="PK242_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HOZ93184.1"
                     /translation="MPRFTPLPLVLACAGLLASCAATDAGPAHAPAAQVAAASPVVVA
                     PAPASAPPAPAAAAADGDTGSEPMLIRGTDRVVAPPPIAVNAPARGGTTSLKFESAPA
                     AEVVRVMLGDLLKVDYVVHPPLSGQITLTTRQPVSPDRALLLLEAALQANGTVMARDS
                     RGTYHVGAPESLKGIVSAPVLAEPGKPLPPGYGSVIVPLEYLGATEMANILRPMVAPD
                     AIVRVDTLRNLLVMRGTRAEAEGWLDLVRTFDVNLLRGMSVGVFQLKHSSAAEVEAAL
                     RLLSGGTTSGAAGAAAAAGAAGASTARPPAAAGRPAAAGTQAPAAAGQPAAPAGLGEA
                     SPLFGALRVIPIERINAVLVVTPRAAYLDEARYWIEQFDRPNLNSVEPQLFVYKVQNG
                     SADHLAELLNGIYGGTTQQSGFSGATGLAPGLTGVGASTNVNSSFASQFGANRNASQT
                     RAWGSTGGLGGTGGLLGGAAGGLGAAGGLGLSGGARGTLGAGTAGTAGAAQGPAVVTT
                     TLGQNVRVMADRINNTLLVHATPAEYERIAATLKRLDIQRAQILIEASIVEVTLGDGL
                     EYGLQWTFNGGLGDGRSGRGVIGAAAAGGTLAADGSTAAGISNAFTYTLRNAAGNISA
                     VLTALANKSLVKVMSSPSLMVLDNHTAAIQVGTQQPVNTGTTISGTSNITTTNIQYKD
                     TGVMLGVTPSVSAGDLVTLDIDQIVTDLGADSSSAATSGYPTFLQRQINSKVAVRSGE
                     TLVLGGLIKDSASSGKAGVPLLSSIPVVGALFGRHRNTADRTELLVILTPRVLRSDED
                     ARAISRELRDRMQGLLQGGAALPAPARH"
     gene            complement(3188..3379)
                     /locus_tag="PK242_03865"
     CDS             complement(3188..3379)
                     /locus_tag="PK242_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93185.1"
                     /translation="MRLLRPFVPLVLLVGSALVACGQRDAPASDSAAPASPPSSAAQR
                     GSFGGGAPKAPASAASAAH"
     gene            complement(3425..4165)
                     /locus_tag="PK242_03870"
     CDS             complement(3425..4165)
                     /locus_tag="PK242_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93186.1"
                     /translation="MNRAGTIVYLAAVGLLTFVLAALWLAPGPQARWRQWQAPAPQAP
                     QLDDVQAAQLRPNPAATANYPGVVQRPLFSPDRRPVAAASAAAPAAPPVAIEQVRLQG
                     LVAGPSLTGVMLEEQGKTRFVRVGETVADWKLERVQARAALFSRAGQQREIALKAVSP
                     EPSSGAANAPAQGRPRAPAAAPPTAAAPAAPAAAPAGVSAPPGDATSAPRAPQPAAAG
                     APAARGGFGGGAGRPRPAAPAGTTNSAR"
     gene            complement(4162..4725)
                     /gene="gspM"
                     /locus_tag="PK242_03875"
     CDS             complement(4162..4725)
                     /gene="gspM"
                     /locus_tag="PK242_03875"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOZ93187.1"
                     /translation="MTTPLARPRPEALWLLIAGALLLALLAWASWTVVAKYRQASAVL
                     AEIEPRHARLAGLLQNQELLAQTDSALQANLIEHVGTAEEDPGQTGNAALQRVRELAG
                     GRGLRVASSQTTAPRDDNGFDRIGLSLRVEGDWPALVALLRELAQQRPALYIHSVQLG
                     IQGFGHQGAPQTLFGNLELYVLKERRP"
     gene            complement(4722..5804)
                     /locus_tag="PK242_03880"
     CDS             complement(4722..5804)
                     /locus_tag="PK242_03880"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOZ93188.1"
                     /translation="MTSTTADRHRPFSLDPAQWPRQWRAAAMLALDGPLLRSLRPASR
                     VALRHADGQTRVWRLVRGQAHAVPGAPGGAELPALQLPAERVLERRLLLPPLAPDDLA
                     QAVQLDVAASSPFGAAQTVHGWSVRRAADQLVQVDVAIASRPQVEQALQAAGFDPAQA
                     PEVWVLPPAADGAGVQPVVLRGFGEDRRERLARQGLTRRLWLLALLLALLAALVITPT
                     ALVRARAQQAQQAFAALQKQAAPQLAQREALMQRLARLQAVGQVYQQQLALPPVLDLL
                     TRALPDGAWLTGLRLEGTKLVLNGQADDAAALVQRLAREPGAHDVRLASPATRGAGAT
                     KETFIIELRLEPRRHGPVQIGEGAAS"
     gene            complement(5824..6708)
                     /locus_tag="PK242_03885"
     CDS             complement(5824..6708)
                     /locus_tag="PK242_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOZ93189.1"
                     /translation="MKVRLLHPPSRTAASAGMALIAVLWIVAALSLLVVGVSATVRQQ
                     IRFVGAERDQVSGQALGEAAIALALQQLQVAPERPRGIVSASVDYAGVPITVELAPLS
                     GLISLNGAAPELLAALLQVAGGLPAAQAQALAASLVEWREGRPELALTTDPAARQPRR
                     FEAVEDLLLVPGIDYGLYARLAPLVSADLGSLARVNPEAAPPGVLAVLAQGQGGAAQQ
                     YLRQRAGGQPGADTSGFNQAFIGTAGTSLYRLTAGVPLEAGKMLHLVQDVALVAGASR
                     GAPWRVLRSRRQIVLTPG"
     gene            complement(6713..7405)
                     /locus_tag="PK242_03890"
     CDS             complement(6713..7405)
                     /locus_tag="PK242_03890"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOZ93190.1"
                     /translation="MNAALGRRRGFTLVEVLIALVLLSLLMLVLTSALRSMGQVEARV
                     EQRIEAADDFRLATQLLGEVLGQASARRHLVQAAGGPAQAPFFEAGPEALAWIGVMPA
                     RFGLGGRHYLRLALEPGEGGARLVLRYAPWNGAPAFSAWGQAEARVLAWPVSALALRY
                     LEPASGQWSPQWPPPGLTTNALPPSLLPAAVELRLDGPAPAWPPLVVALRANYATDPS
                     AVLGGFGGGAPR"
     gene            complement(7402..7845)
                     /locus_tag="PK242_03895"
     CDS             complement(7402..7845)
                     /locus_tag="PK242_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOZ93191.1"
                     /translation="MSLPRPPGGEGRGEGARQRGFSLLEILVAFAIAAMALGLLYQVS
                     GNNARQAGGLLQHERAMALAQSLLAAHETVPARGLDERGEGAGYGWYVHSRPYPTPAG
                     NAAPQAARLHELRIDVRWHDGTAERSFELSSLRPERRPQPGEVIR"
     gene            complement(8052..8564)
                     /locus_tag="PK242_03900"
     CDS             complement(8052..8564)
                     /locus_tag="PK242_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOZ93192.1"
                     /translation="MRQARAAIINIASKAGFRPPAPARARGFTLIELLVVIALMGLIA
                     GLVPLAFGRLYESAQYRDTVRAVLTELRAARQQAMSQGAETRFAVDLRARTFGVEGGP
                     QHAVPEPLVMRAVMAAETADADGRLAIHFLPRGGASGGSVDLLRPSGSGVRLRVDWFT
                     GRVEQEAIEP"
     gene            complement(8614..9051)
                     /gene="gspG"
                     /locus_tag="PK242_03905"
     CDS             complement(8614..9051)
                     /gene="gspG"
                     /locus_tag="PK242_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOZ93193.1"
                     /translation="MTPFARRQLARAARGFTLIEMLVVIAIIALLAGLVGPAVMDRLG
                     GAKSKTAGIQIADLDKALEIFKLDVGRYPSNAEGLQALVTRPSSANGWNGPYLKGGLP
                     ADPWGNAYRYANPGPNGGLEILSLGADNAPGGEGENADVRNRQ"
     gene            complement(9242..10471)
                     /locus_tag="PK242_03910"
     CDS             complement(9242..10471)
                     /locus_tag="PK242_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOZ93194.1"
                     /translation="MPEFTWRAAAADGRLIEGRSEAPAPDAVLRQLRERGLTPIQVGT
                     ATPQAAASAALGRGGQRTLKGPVTRADVLAFTGELSIMLRAGLALDNALRVLIDMSAK
                     PSVRSLLEGVLEDVKGGATFSRALTKRSELFGDFYLNMVRSGEASGQMSQVLERLVAH
                     MERLRALRESVISATLYPAILLGVAVLSLVGMLGFVVPQFERLFKDMGEALPLPTRIV
                     MALGQGFRQHGLLLAIGALLLGWLGWRWLRSPAGRAWWQRRALRLPVVGPLLYKYELT
                     LFARSLGTLLGNGVPLLTALHIATDTVGNLNLRQPLARMAPQVKEGARMVRAMESTGV
                     FEPLAINLVRVGEETGRIGPMMLELSEVLDREVETGIKRALTLLEPILILLLGVLIAA
                     IIVSILLGILSINDLAG"
     gene            complement(10575..12254)
                     /gene="gspE"
                     /locus_tag="PK242_03915"
     CDS             complement(10575..12254)
                     /gene="gspE"
                     /locus_tag="PK242_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOZ93195.1"
                     /translation="MHWPDYGERLVAAGKLPARDLERALAAQREMGGSLDAVLVSLGL
                     VSELDAAQALAEHLQLPFVPGEGFPEPLPDIDGLQPEFLQSHQVLPLGVDGHDLVVAM
                     RAPQDAFVVKALHLSTGLQVRPSVALASDIDKALRRQQDEAEAQTPDQGAAEAGDAGD
                     FVEHLRDLASEAPVIRLVNSLIGRVIELRASDIHLEPFDDGLHVRYRIDGVLHPGELV
                     PAQQGAAVSSRVKLLAHLDIAERRLPQDGRIKTRVKGRELDLRVSTVPTVWGESVVMR
                     VLDRASVRFGLGDMGFEADTLARFNQLIHRPHGILLVTGPTGSGKTTTLYAALSTLDA
                     ASQKIITVEDPVEYQLEGINQIQVHSQINLTFANALRSILRQDPDIIMIGEMRDGETA
                     QIAVQSSLTGHLVLSTLHTNTAASAVVRMQDMGVERYLITSTVNGVLAQRLVRRLCPH
                     CRTPVQPSPELWRSSGLARFVPAGQPIYQASGCEHCRGTGYQGRSAIHELLVVDETLR
                     SAILQGQDASALQALAVQAGMHTLYDDGLRKVAAGVTSLDELLRVTQDQGE"
     gene            complement(12437..13624)
                     /locus_tag="PK242_03920"
     CDS             complement(12437..13624)
                     /locus_tag="PK242_03920"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOZ93196.1"
                     /translation="MSSTPSAPRYQRNFGLDLLRSVAIGVVLMNHGYLGFFVSTGLSK
                     WEGWRAALSACAVFSIEWLFVLSGYLIGSMMIRSFETRGGWWASARDFWLRRWFRTIP
                     NYYLFLLINALIAWWGIEEGRFAWSFAVFSQNLAWAQTKPYFFAEAWSLALDEWFYFL
                     MPLLLGGVALLRRLERRQLFWAVALLLITVPTVLRSVVTPSAHFFAWDDDIRRVTLMH
                     LDATGWGVAAAILNRWHPGWWARRQGLKALLGLALTLGAVAAMHYFMRVDWRGFAGGR
                     FNDLALIVAPGVGTVLILPWLSSHQPAAGWGRWLAARIGDYSYSIYLCHFPLMLLLIH
                     ALKLWGGALGPIVGWVVLVWLVAVLAVSALVFHGFEKPVSDLRERFTRRVQAGPFSVP
                     TAT"
     gene            complement(13621..14421)
                     /locus_tag="PK242_03925"
     CDS             complement(13621..14421)
                     /locus_tag="PK242_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ93197.1"
                     /translation="MNIAASIVSHGHGAAVEALLAQLAALGEPGLARVIVTFNVPEPA
                     SAERLRGRHWPFQLHLVDNPAPLGFGANHNRAFALDARIDGGQPLFAVLNPDLRCLVN
                     PFPPLVAELQSDPRVGAAYPVQTDARGCRQDHERLRPTPLRLLRRHLLGRRMEVGAGQ
                     APDWVNAAFLLLRRPAFEAIGGFDEAFHMYCEDVDFCLRLQLAGWRLRRADAATVEHQ
                     AQRASRRRLRHLLWHARSLIRLWRSPAWRAWRASESAPASATDRAHRS"
     gene            complement(14443..15000)
                     /gene="rfbC"
                     /locus_tag="PK242_03930"
     CDS             complement(14443..15000)
                     /gene="rfbC"
                     /locus_tag="PK242_03930"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOZ93198.1"
                     /translation="MHVIPTALVGVLVLEPRVFGDDRGFFMESYNRRSFAQATGLDID
                     FVQDNHSRSRKGVLRGLHYQLRQPQGKLVRVTHGAVFDVAVDIRRGSATFGRWVGVEL
                     SADNHRQLWVPPGLAHGFVVLSDSADFLYKTTDYYAPEHERSIAWDDPAIGIDWPLAA
                     HGITAPLLSAKDRAGQPLSRAELVD"
     gene            complement(15032..15610)
                     /locus_tag="PK242_03935"
     CDS             complement(15032..15610)
                     /locus_tag="PK242_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ93199.1"
                     /translation="MHPPAPMPPSIRKARRIDGRTLALRDAGESDAAFIHALRTDPVR
                     ARFLSAVPPDPSAQTEWLRRYAADSSQAYFVIADGAAGEPLGTVRLYGARGDAFSWGS
                     WLLKAGLPSHCAIESALMTYHYGLWLGFRSAYFEVRQDNLSVWRFHEHFGATRIGADD
                     RHFHYELAADALTQALQRYRRYLPHGIRVSRD"
     gene            complement(15610..17466)
                     /locus_tag="PK242_03940"
     CDS             complement(15610..17466)
                     /locus_tag="PK242_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93200.1"
                     /translation="MPRPEPLSPALSKWWLRAGMALPLLMLGANLLAWLRWGTDLPYL
                     DDWRVYDDGSALSLDPRHLFRAVNNTISPVGLTLDVLAQRWLGGNPLPYQALSMLAVL
                     GGLLWLQWRLLGWAVRDRRQQALLFAFCVPMLQSGSYWGEQSLAYHQALPLLALLGAM
                     AWALPRPRRRAALGVFVLGAVAGLSYISGAIAALVLGACWLVLARVLRTHPTPALATR
                     IAGPGGALLLAGGLTSAMQFHLTRGDAQARDRQYMGLTYPDSPDFWAFLAGKIGRATG
                     HGFASLPLELIWVALVLLTLLAALGLTGRAALAPGRVPSVPRRRVAVVFLPLCAIVLT
                     YLVLVSLGRAGFRDPGIQGAGGVFRFGYERFHFFWVTLLFPWVAAALVPRRRTRAVPV
                     LVGTLLTALALAAVRGVFDVPSFYRSASAYRASELRCLGRQLGSGQPIRCPGYDMMGM
                     TDLTRAYIHARDIQASFVRYLPVQTRDGFGRELLDWHAAAGTEPGPWAHVRRRADGGL
                     QSEGDAQLTVAWPDAALSRRCRTLGVQLDIVTAHAETAQLYYRWSGQQSDSEARSVRQ
                     AYQPGVDGHARLELSIDSPVGFEPRLRLDPVDGQGSFRLRELRVTCRLASQP"
     gene            complement(17453..17824)
                     /locus_tag="PK242_03945"
     CDS             complement(17453..17824)
                     /locus_tag="PK242_03945"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOZ93201.1"
                     /translation="MAREALRYLLAGGANTALTYLLYLALLRLLDYRWAYVLAFLAGV
                     ALSFVLLRHVVFARPGKRFSWVYVTASHGVQLGLGLVVVEAWVAWLRGPAWLAPLAAA
                     LVCVPVIFVLQRWIFTPHAAP"
     gene            complement(17824..18285)
                     /locus_tag="PK242_03950"
     CDS             complement(17824..18285)
                     /locus_tag="PK242_03950"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HOZ93202.1"
                     /translation="MPASSVPSAPRVIDSRVTGVRLHELREVKDARGDLCVAEVGADL
                     PFAVRRSFLVYRVPSAELRGAHAHRRCGQFLQAVAGSVHVIADDGRQREEFCLDRPSL
                     GLYLPPMTWGIQHRYTQGAVLLVLASDPYDPADYIRDYDEFLALRRAGGAA"
     gene            complement(18295..19227)
                     /locus_tag="PK242_03955"
     CDS             complement(18295..19227)
                     /locus_tag="PK242_03955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ93203.1"
                     /translation="MLSLIIPVYRNEASIPELLTELQGLHQALTGALEVVFVVDGSPD
                     RCHALLRQQLPASGLNATLVLLTRNFGAFAAVRMGLEQARGDTFAVMAADLQEPPELV
                     LQMQAELARGQADVVVAERTLRHDPLLQRWQAQLFWAAYRRWVVPEMPPGGVDIFACN
                     RAFRDALLGLGESRSSLIAQIFWLGFRRASVPYERQPRRHGQSAWTWRKKLAYLADSV
                     FAFTDLPVRLLTRVGALGLLVAGVLGAVVLFGKLSGLIGVPGYAMTLIAILFFGALNL
                     FSLGIVGTYAWRAYENTKQRPQAVALRVDRFQSH"
     gene            complement(19230..20339)
                     /locus_tag="PK242_03960"
     CDS             complement(19230..20339)
                     /locus_tag="PK242_03960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOZ93204.1"
                     /translation="MASAPQVAFFSPFGAAFGVDLGACLQRVVDSGWYVLGPQVREFE
                     TAFARYVGVRHAVGVANGTDALTLALRALGIQPGQRVLTVANAGCYSSTAMAQVGADP
                     GWVDVDPARLTMAPEALAAALAEGRVAAVIVTHLYGQMADMPRLTALCQAAGVPLIED
                     CAQAHGAALHGRCAGAWGTLACFSFYPTKNLGALGDGGAVLTDDAALAERLRALRQYG
                     WTRKYEVGLPGGGNSRLDELQAAVLLAKLPHLEAANAERRAIAAGYSAAFAGLPLDCP
                     PPPGPDHAMHLYVVRTPHRAALQAHLAAHGVASDIHYPLADHRQPVRAAQPLPHLPVT
                     EAACAEVLSLPCYPGLTAPQGARVIEVVRQFFAAA"
     gene            complement(20341..>20570)
                     /locus_tag="PK242_03965"
     CDS             complement(20341..>20570)
                     /locus_tag="PK242_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOZ93205.1"
                     /translation="LDTGTHDSLLDAGQFIATLERRQGLKIACPEEVAWRKRFIDDAQ
                     LERLAAPLAKSGYGQYLRRLLQEPAQPAERR"
BASE COUNT         3112 a         7058 c         7766 g         2634 t
ORIGIN      
        1 aaagtggttg caagacccaa gcgaatcttg gaaatgcccg gtgatgctaa cacggcccgt
       61 acggactttt caatcagccc gcagtgacac gggggaacgc acggcgccga atgttgcaaa
      121 tttcgcacag tcgtcaccag gcggggttgc ctgtgcggtc agaagttctg gtagtaccac
      181 cagttgccgt cttcgcgcac ccaggtttcg tcgatcacgc ccgccacctc ctggccctgg
      241 aagggtggcg tcagcacacg ggtggtcagg cggatgcggg ccttgcagcg ctcggcctcg
      301 caactgacct tgtgcacctg cacgccacgc cactggccgg caaaaccgaa ggtccggcgg
      361 tagtcggcct gcttgacgat ggcgcggtag gcgggctggg tgtaggtcca ggccttgggg
      421 aagtcgccct tgatgagggc gtcccagcgt tcttcgacgc gctgggcgac gatttcttcg
      481 ggcgttctgg gcgtgagcgc ggcgcagccg gccagcgcca gtgcggcgac ggccagcaga
      541 ccgcaacggc gcgcaacgcg caggcgcgag gccgtggggg cggaaatggg catgggtggt
      601 tctccggaaa tatggaacaa cgagagcaat cgtgccttgg gtcggccggc gcgcggctca
      661 gtgccgggcc ggcgccggca gcgccgcgcc gccctgcagc aggccctgca tgcggtcgcg
      721 cagttcgcgg ctgatggcgc gcgcgtcttc gtcggagcgc agcacgcgcg gggtcaggat
      781 gaccagcagc tcggtgcggt cggcggtgtt gcggtggcgc ccgaacagcg cgcccaccac
      841 cgggatggac gacagcaggg gcacgccggc cttgccgctg ctggcgctgt ccttgatcag
      901 gccgcccagc accagggttt cgcccgagcg caccgccacc ttggagttga tctggcgctg
      961 caggaaggtg gggtagcccg aggtggcggc cgaggacgag tcggcgccca ggtcggtgac
     1021 gatctggtcg atgtccagcg tgaccaggtc gcccgcgctg accgagggcg tgacgcccag
     1081 catgacgccg gtgtccttgt actggatgtt ggtggtggtg atattgctgg tgccgctgat
     1141 ggtggtgccg gtgttgaccg gctgctgggt gccgacctgg atggcggcgg tgtggttgtc
     1201 gagcaccatc agcgaggggc tggacatgac cttgaccagc gacttgttgg ccagcgcggt
     1261 gagcaccgcc gagatgttgc cggcggcgtt gcgcagggtg taggtgaagg cgttgctgat
     1321 gccggccgcg gtgctgccgt cggcggccag ggtgccgccg gccgccgcgg caccgatgac
     1381 gccgcggccg ctgcgaccgt cgcccaggcc gccgttgaag gtccattgca ggccgtattc
     1441 gaggccgtcg ccgagggtga cttcgacgat cgaggcttcg atcaggattt gcgcgcgctg
     1501 gatgtccagc cgcttgaggg tggcggcgat gcgttcgtat tcggccgggg tggcgtgcac
     1561 cagcagggtg ttgttgatgc ggtcggccat gacgcgcacg ttctgcccca gggtggtggt
     1621 gaccaccgcc ggcccctgcg ccgcgccggc ggtgcctgcg gtgccggcgc cgagggtgcc
     1681 gcgcgcgccg ccgctcaggc ccaggccacc ggccgcgccc agaccgccgg cggcgccgcc
     1741 cagcagcccc ccggtgccgc ccagaccgcc cgtgctgccc caggcccgcg tctgcgaggc
     1801 gttgcggttg gcgccgaact ggctggcgaa gctgctgttg acgttggtgc tggcgccgac
     1861 gccggtgagg cccggcgcca ggccggtggc gccgctgaag ccgctttgct gggtggtgcc
     1921 gccgtagatg ccgttgagca gctcggccag gtggtcggcc gagccgttct gcaccttgta
     1981 gacgaacagt tgcggctcga cgctgttgag gttggggcgg tcgaactgct cgatccagta
     2041 gcgcgcctcg tccaggtagg cggcgcgcgg ggtgaccacc agcacggcgt tgatgcgctc
     2101 gatggggatg acgcgcaggg cgccgaacag ggggctggct tcgcccaggc cggccggcgc
     2161 ggccggctgg ccggcggccg ccggggcctg ggtgccggcc gcggccggcc ggcccgccgc
     2221 ggcggggggg cgggccgtgc tggcgccggc ggcaccggcg gcggccgcgg caccggcggc
     2281 gcccgaggtg gtgccgcccg agagcaggcg cagcgcggcc tcgacctcgg cggccgagct
     2341 gtgcttgagc tggaacacgc cgaccgacat gccgcgcagc aggttgacgt cgaaggtgcg
     2401 caccaggtcg agccagcctt cggcttcggc ccgggtgcca cgcatgacca gcaggttgcg
     2461 cagggtgtcg acgcgcacga tggcgtcggg cgcgaccatg ggccgcagga tgttggccat
     2521 ttcggtcgcg cccaggtatt cgagcggaac gatgacgctg ccgtagccgg gtggcagcgg
     2581 cttgccgggt tcggccagca ccggggcgct gacgatgccc ttgagcgatt cgggcgcgcc
     2641 gacgtggtag gtgccgcggc tgtcgcgcgc catgacggtg ccgttggcct gcagcgcggc
     2701 ttcgagcagc agcagggcgc ggtcggggct gacgggctgg cgcgtggtga gggtgatctg
     2761 gccgctgagc ggggggtgaa cgacgtagtc gaccttgagc aggtcgccca gcatgacgcg
     2821 caccacttcg gcggcggggg cggattcgaa cttgaggctg gtggtgccgc cgcgcgccgg
     2881 ggcgttgacc gcaatgggcg gcggcgccac cacgcggtcg gtgccgcgga tcagcatcgg
     2941 ctcgctgccg gtgtcaccgt cggccgcggc ggcggcaggg gcgggcggcg cgctggcggg
     3001 ggccggggcg acgaccacgg gcgacgctgc ggcgacctgg gccgcgggcg cgtgggccgg
     3061 gccggcatcg gtggcggcgc aggacgccag caggccggcg caggccagca cgaggggcaa
     3121 aggggtgaaa cggggcatgg gcttcggtgt cgatggggtc gcgaagacgg ggcgggggct
     3181 gcgcaggtca gtgcgccgcg ctggcggcgc tggcgggcgc cttgggcgcg ccgccgccga
     3241 aactgccgcg ctgggcggca ctggaaggcg ggctggcggg cgcggccgaa tcggaggcgg
     3301 gggcgtcacg ctggccacag gccaccaggg cgctaccgac cagcaacacg agcggaacga
     3361 aggggcgaag caggcgcatg gcagtacgaa gcggtgagtg aggatgggca ggggcggtcc
     3421 aggatcatcg cgccgagttg gtggtgccgg caggcgcggc gggcctgggg cggccggcac
     3481 caccgccgaa gccgccgcgg gcggccggcg cacccgcggc agcgggctgg ggcgcgcggg
     3541 gcgccgaagt ggcgtcgccg ggaggggcgg acacgcccgc cggcgcagca gcaggggcgg
     3601 caggggcggc agcggttggg ggcgccgcgg cgggcgcgcg cgggcgtccc tgcgctggcg
     3661 cattggcggc accggacgac ggctcggggc tgaccgcctt cagggcgatc tcgcgctgct
     3721 ggcctgcacg gctgaacagg gcggcgcggg cctgcacgcg ctcgagcttc cagtcggcga
     3781 cggtttcgcc cacacgcacg aagcgggtct tgccctgctc ttccagcatg acgccggtca
     3841 aggatggtcc ggccaccagg ccttgcagcc ggacctgttc aatggccacg gggggcgccg
     3901 ccggcgcggc ggcgctggcg gcggcgacgg ggcggcggtc ggggctgaac agcgggcgct
     3961 ggacgacgcc ggggtagttg gcggtcgccg ccgggttggg ccgcaactgg gcggcctgca
     4021 cgtcgtccag ctggggggcc tggggtgcgg gcgcctgcca ctggcgccag cgcgcctgtg
     4081 gaccgggcgc cagccacagc gccgccagca cgaaggtcag caggccgacg gcggcaaggt
     4141 agacgatggt gccggcgcgg ttcatggccg acgctccttg agcacgtaga gctccaggtt
     4201 gccgaacagg gtctggggcg cgccctggtg gccaaagccc tggatgccga gttgaacgct
     4261 gtggatgtag agcgcggggc gttgctgcgc cagctcgcgc agcagcgcca ccagggccgg
     4321 ccagtcgcct tcgacgcgca gcgacaggcc gatgcggtcg aagccgttgt cgtcgcgcgg
     4381 ggcggtggtc tggctgctgg ccacacgcag gccgcgcccg ccggcgagtt cgcgcacgcg
     4441 ctgcagggcg gcgttgccgg tctggccagg gtcttcctcg gccgtgccga catgttcaat
     4501 caaattggcc tgcagcgcgc tgtcagtctg tgcaagcagc tcttgatttt gtagcaaacc
     4561 ggcaagccgg gcatggcgcg gctcgatctc ggccagcacg gcgctggcct ggcggtactt
     4621 ggccaccacg gtccaggacg cccaggccag cagcgccagc agcagcgcgc cggcgatcag
     4681 cagccacagg gcttcggggc gtgggcgggc gagcggggtg gtcatgaggc ggcgccctcc
     4741 ccgatctgca ccgggccatg gcggcgcggt tcgagccgga gttcgatgat gaaggtctcc
     4801 ttggtggcgc cggcgccgcg ggtggcgggc gaggccaggc gcacgtcgtg ggcgccgggt
     4861 tcgcgcgcca gccgctgcac cagggcggcg gcgtcgtcgg cctggccgtt gagcaccagc
     4921 ttggtgcctt ccaggcgcag gccggtgagc caggcgccgt cgggcagggc gcgggtgagc
     4981 aggtccagca ccggcggcag ggccagttgc tgctggtaga cctggccgac ggcctgcagg
     5041 cgcgccagcc gctgcatcag cgcctcgcgc tgggccagct ggggcgcggc ctgcttttgc
     5101 agcgcggcaa acgcctgctg ggcctgctgg gcgcgcgccc gaaccagcgc cgtgggggtg
     5161 atgaccaggg ccgccagcag cgccagcagc agcgccagca gccacagccg gcgtgtcagg
     5221 ccctggcgcg ccaggcgttc gcgccggtct tcgccgaagc cgcgcagcac caccggctgc
     5281 acgccggcgc cgtcggccgc cggcggcagc acccagactt cgggcgcctg cgcggggtcg
     5341 aagccggccg cctgcagcgc ctgctcgacc tgcgggcgcg aggcgatggc gacgtcgacc
     5401 tggacgagtt gatcggcggc gcggcgcacc gaccagccgt gcacggtctg ggcggcgccg
     5461 aacgggctgg aggcggccac gtccagctgc acggcctgcg ccagatcgtc gggcgccagg
     5521 gggggcagca gcaggcggcg ctccagcacg cgctcggccg gcagttgcag ggccggcagc
     5581 tcggccccgc cgggggcacc cggcacggca tgggcctggc cgcgcaccag gcgccagacg
     5641 cgggtctgac cgtcggcgtg gcgcagcgcc acgcggctgg ccggccgcag gctgcgcagc
     5701 agcgggccgt ccagcgccag catggcggcg gcgcgccact ggcgcggcca ctgggcgggg
     5761 tcgaggctga agggacggtg gcggtcggcg gtggtggagg tcatgcgggg gaaaggtccg
     5821 ggctcatccg ggtgtcagga cgatctggcg gcggctgcgc agcacgcgcc agggcgcgcc
     5881 gcgcgatgcg ccggccacca gggccacgtc ctggacgagg tgcagcattt tgcctgcttc
     5941 cagcggcacg ccggccgtga ggcggtaaag agacgtgcct gcggtgccga tgaacgcctg
     6001 attgaaaccg ctggtgtcgg ccccgggctg accgccggcg cgctggcgca ggtattgctg
     6061 ggcggcgccg ccctggccct gcgccagcac cgccagcacg ccaggcggcg cggcttcggg
     6121 gttgacgcgg gccaggctgc cgaggtcggc actgaccagc ggcgccagcc gggcgtacag
     6181 gccgtagtcg atgccgggca ccagcagcag gtcctcgacg gcttcgaagc ggcgcggctg
     6241 gcgggcggcg gggtcggtgg tgagggccag ctcgggtcgg ccttcgcgcc attcgaccag
     6301 gctggcggcc agcgcctggg cctgggctgc cggcaggccg ccggccacct gcagcagcgc
     6361 cgccagcagc tcgggcgcgg cgccgttgag cgagatcagg ccgctgagcg gcgccagttc
     6421 gacggtgatg ggcacaccgg cgtagtcgac gctggcgctg acgatgccac gcgggcgctc
     6481 gggcgcgacc tggagctgct gcagcgccag cgcgatggcg gcttcgccca gcgcctggcc
     6541 gctgacctgg tcgcgttcgg caccgacgaa ccggatctgc tggcgcacgg tggcgctgac
     6601 gccgaccacc agcaggctga gggcagcgac gatccacagc acggcgatca gcgccatgcc
     6661 ggcgctggcg gcggtccggg atggggggtg caggaggcgg accttcatgg catcaacgcg
     6721 gggcaccgcc accgaagccg cccagcacgg ccgagggatc ggtggcgtag ttggcgcgca
     6781 gcgccaccac cagcggcggc caggccggtg ccgggccgtc caggcgcagt tcgacggccg
     6841 ccggcagcag gctgggcggc agggcgttgg tggtcaggcc gggcggcggc cactgcgggc
     6901 tccactggcc gctggccggc tccaggtagc gcagcgccag cgcgctgacc ggccaggcca
     6961 gcacgcgcgc ctcggcctga ccccaggcgc tgaaggcggg cgcgccgttc cagggggcgt
     7021 agcgcagcac caggcgcgcg ccgccctcgc ccggctccag cgccaggcgc aggtaatgcc
     7081 tgccacccag gccgaagcgc gccggcatga cgccgatcca ggccagcgcc tcgggcccgg
     7141 cctcaaagaa cggcgcctgg gccgggccgc cggccgcctg caccaggtgg cggcgcgccg
     7201 acgcctggcc cagcacttcg cccagcaact gggtggcgag gcgaaagtcg tcggcggcct
     7261 cgatgcgctg ctcgacgcgc gcttcgacct ggcccatgct gcgcagcgcg ctggtgagca
     7321 ccagcatcag cagcgacagc aagaccaggg cgatcagcac ctcgaccagg gtgaagccgc
     7381 ggcggcggcc gagcgcggcg ttcatcggat cacctcaccc ggctgcggcc gtcgctcggg
     7441 gcgcaggctg ctgagctcga agctgcgctc ggcggtgccg tcgtgccagc gcacgtcgat
     7501 ccgcagctcg tgcaggcgcg ccgcctgcgg cgccgcgttg ccggccggcg tggggtaggg
     7561 tcggctgtgc acgtaccaac cgtagccggc gccttcgccg cgctcgtcca gcccacgcgc
     7621 cggcacggtt tcgtgggcag ccagcagcga ctgcgccagc gccatggcgc gctcgtgctg
     7681 cagcagaccg cccgcctggc gcgcgttgtt gccgctgacc tggtagagca ggcccagcgc
     7741 catggcggca atggcgaagg cgaccaggat ttcgagcagc gaaaagccgc gctggcgcgc
     7801 cccctcaccc ctgccctctc ccccgggggg gcgagggagc gacaccgcca gcgggggagc
     7861 gtgggggcaa accccttccg ccgccgggcc gccccaaggc ggaagcggcc ccctcggggg
     7921 gcagcgcacc tcgcgcagcg ggggagcgtg ggggcaaacc ccttccgccg ccgggccgcc
     7981 ccaaggcgga agcggccccc tcggggggca gcgcacctcg cgcagcgggg gagcgtgggg
     8041 gcgaacaagc ctcatggctc gatggcctcc tgctcgacgc ggccggtgaa ccagtccacg
     8101 cgcaggcgca cgccgctgcc cgaggggcgc agcagatcga cgctgccacc actggcaccg
     8161 ccgcgcggca ggaagtggat ggccaggcgg ccgtcggcgt cggcggtttc ggccgccatg
     8221 acggcgcgca tcaccagggg ctcgggcacg gcgtgctgcg ggccgccttc gacgccgaag
     8281 gtgcgcgccc gcagatcgac ggcgaagcgg gtctcggcgc cctggctcat cgcctgctgg
     8341 cgcgcggcgc gcagctcggt cagcacggca cgcacggtgt cgcggtactg cgccgattcg
     8401 tacaggcggc cgaaggccag cggcaccagg ccggcgatca ggcccatgag ggcgatcacc
     8461 accagcagct cgatcagggt gaagccgcgc gcgcgcgccg gcgccggtgg tcgaaaccct
     8521 gccttcgatg ctatatttat aatagctgct cgcgcttgcc gcataagcgc tagagcctga
     8581 tttttcttat aagttcaggt ggatcgccag gatttactga cggttgcgca cgtcggcgtt
     8641 ttcgccctcg ccgccggggg cgttgtcggc gcccagcgac aggatttcca gcccgccatt
     8701 ggggccgggg ttggcgtagc ggtacgcgtt gccccagggg tcggcgggca ggccgccctt
     8761 gaggtagggg ccgttccagc cgttggccga ggacggccgg gtgaccagcg cctgcaggcc
     8821 ctcggcgttg ctggggtagc ggccgacgtc gagcttgaag atttcgagcg ccttgtccag
     8881 gtcggcgatc tggatgccgg cggtcttgct cttggcgccg cccaggcggt ccatcacggc
     8941 cgggccgacc aggccggcca gcagggcgat gatggcgatc accaccagca tctcgatcag
     9001 ggtgaagccg cgcgcggcgc gggcgagttg acggcgagca aagggggtca tgaaaaatgt
     9061 cctctcaaca gggcaagcta tttgaaatgg gttgtgccgc gcaagcgcac gcgtccgatg
     9121 gccgcagagc agaccaagcc agcgatcgcc ctcgccggga tcccccggcc gacggcgatg
     9181 cccccctgga gggaggaggc gcaacagcgc gcagcgcgtg gagccaaggg ggtgggccat
     9241 gtcatccggc aaggtcgttg atggacagaa tgcccagcag gatcgagacg atgatggcgg
     9301 cgatcagcac gcccagcaac aggatcagga tgggctccag cagcgtgagg gcgcgcttga
     9361 tgccggtttc gacctcgcgg tcgagcacct cggacagctc cagcatcatc gggccgatgc
     9421 ggccggtttc ctcgcccact cggaccaggt tgatggccaa aggttcaaac acgccggtgc
     9481 tttccatggc gcgcaccatg cgggcgccct ccttcacctg cggcgccatt ctggccagcg
     9541 gctggcgcag attgaggttg cccacggtgt cggtggcgat gtgcagcgcg gtcagcagcg
     9601 gcacgccgtt gcccagcagg gtgccgagcg agcgggcaaa cagcgtcagc tcgtatttgt
     9661 agagcagcgg accgaccacc ggcaggcgca gcgcgcggcg ctgccaccag gcgcgcccgg
     9721 ccggcgagcg cagccagcgc cagcccagcc agcccagcag cagcgcgccg atggccagca
     9781 gcagcccgtg ctggcgaaag ccctgcccga gcgccatgac gatgcgcgtg ggcagcggca
     9841 gcgcttcgcc catgtccttg aacaggcgct cgaactgcgg caccacgaag cccagcatgc
     9901 cgaccagcga cagcacggcc acgcccagca ggatggccgg atacaaggtg gcggaaatga
     9961 cgctttcgcg cagggcgcgc agccgctcca tgtgcgccac caggcgttcg agcacctgcg
    10021 acatctggcc gctggcctcg cccgagcgca ccatgttgag gtagaagtcg ccgaacagct
    10081 cgctgcgctt ggtcagcgcg cgactgaagg tggcgccgcc cttgacgtct tccagcacgc
    10141 cttccagcag gctgcgcacg ctgggcttgg cgctcatgtc gatcagcacg cgcagcgcgt
    10201 tgtccagcgc caggccggcg cgcagcatga tggagagctc gccggtgaag gccagcacgt
    10261 cggcgcgcgt gaccggcccc ttgagcgtgc gctggccgcc gcggcccagc gccgccgaag
    10321 ccgccgcctg cggcgtggcg gtgccgacct ggatcggggt caggccgcgc tcgcgcagct
    10381 ggcgcagcac ggcgtcgggc gcgggcgctt cgctgcgccc ctcgatcagc cggccatcgg
    10441 cggcggcggc acgccaggtg aattcaggca tcagacgcag gccacggcgc aggcgccgcc
    10501 gttcgtgccc gctgccgcag acagcaacgg gttggggtga ggaaaccgcc gcggggcagg
    10561 cgcaggtgcg cgcatcactc cccctgatcc tgcgtcacgc gcagcagttc gtccaggctg
    10621 gtgacgccgg cggccacctt gcgcagaccg tcgtcgtaca gggtgtgcat gccggcctgc
    10681 accgccagcg cctgcagcgc gctggcgtcc tgaccctgca ggatggcgct gcgcagcgtc
    10741 tcgtcgacca ccagcagttc gtggatggcg ctgcggccct ggtagccggt gccgcggcag
    10801 tgctcgcagc cgctggcctg gtagatgggc tggccggccg gcacaaagcg cgccaggccg
    10861 gagctgcgcc acagttcggg cgacggctgc accggcgtcc tgcagtgcgg gcacaggcgg
    10921 cgcaccaggc gctgcgccag cacgccgttg acggtggagg tgatgaggta gcgctccacc
    10981 cccatgtcct gcatgcgcac cacggcgctg gcggcggtgt tggtgtgcag ggtggacagc
    11041 accaggtggc cggtcaggga cgattgcacg gcgatctggg cggtttcgcc gtcgcgcatc
    11101 tcgccgatca tgatgatgtc ggggtcttgc cgcaggatgg agcgcagcgc gttggcgaag
    11161 gtcaggttga tctggctgtg cacctggatc tggttgatgc cttcgagctg gtattcgacc
    11221 gggtcctcga ccgtgatgat cttctggctg gccgcgtcca gcgtggacag tgcggcgtac
    11281 agcgtggtgg tcttgcccga gccggtgggg ccggtgacca gcaggatgcc gtgcggccgg
    11341 tggatcagct ggttgaagcg cgccagcgta tcggcctcga accccatgtc gcccaggccg
    11401 aagcgcacgc tggcgcggtc gagcacgcgc atgaccacgc tttcgcccca cacggtgggc
    11461 acggtggaga cgcgcagatc cagctcgcgc cccttgacgc gggtcttgat gcggccgtcc
    11521 tgcggcaggc ggcgctcggc gatgtccagg tgcgccagca gcttgacgcg cgagctgacg
    11581 gcggcgccct gctgggccgg caccagctcg cccgggtgca gcacgccgtc gatgcggtag
    11641 cgcacgtgca ggccgtcgtc gaagggctcg aggtggatgt ccgacgcgcg cagctcgatc
    11701 acgcggccga tcaggctgtt gaccaggcgg atcaccggcg cctcgctggc gaggtcgcgc
    11761 aggtgctcga cgaaatcgcc ggcatcgccg gcctcggccg ccccctgatc gggcgtctgc
    11821 gcctcggcct cgtcctgctg gcgccgcagc gccttgtcga tgtccgaagc cagcgccacg
    11881 ctgggacgca cctgcaggcc ggtgctcagg tgcagcgcct tgacgacgaa ggcgtcctgc
    11941 ggcgcgcgca tggccaccac caggtcgtgc ccgtccacac ccagcggcag cacctggtgg
    12001 gattggagga attcgggttg caggccgtcg atgtcgggca gcggctcggg aaagccctcg
    12061 cccggtacga agggcagttg caggtgctcg gccagggcct gcgcggcgtc cagctcggac
    12121 accagcccca ggctgaccag cacggcgtcg agcgagccgc ccatctcgcg ctgcgccgcc
    12181 agcgcgcgct ccaggtcgcg cgccggcagc ttgccggcgg ccaccaggcg ctcgccatag
    12241 tcaggccagt gcatggacgg cgcggacagg tcggaagcgg gtcgggtgga cggcaggggg
    12301 atgggttcgg ttcggttcat gtggctgggg cgcgcgcatc accgggcggg ccggcgcacc
    12361 agcggggatt ctacggggcg gccccagggt ctttcgcctg ggtcgcgatg acgacccccc
    12421 cgccgcgccg taaccactac gtcgccgttg gcacactgaa gggccctgcc tggacacgcc
    12481 gggtgaaacg ctcgcgcagg tcggagacgg gcttttcgaa cccgtggaac accagcgccg
    12541 acaccgccag cacggccacc agccagacca gcaccaccca gccgacgatc ggccccagcg
    12601 ccccgcccca gagcttgagc gcatggatca ggagcagcat gagcgggaaa tggcacaggt
    12661 agatcgagta cgaatagtcg ccgatgcgcg ccgccagcca gcggccccag ccggcggcgg
    12721 gctgatggct ggacagccag ggcaggatca gcaccgtgcc gacgccaggg gcgacgatca
    12781 gcgccaggtc gttgaagcgc ccgccggcga agccgcgcca gtccacccgc atgaaatagt
    12841 gcatggcggc cactgccccc agggtcaggg ccaggccgag cagcgccttg aggccctgcc
    12901 ggcgcgccca ccagccgggg tgccagcggt tgagaatggc cgcggccaca ccccaaccgg
    12961 tggcgtccag gtgcatcagg gtgacgcggc ggatgtcgtc gtcccaggcg aagaaatggg
    13021 ccgacggcgt gaccacgctg cgcagcacgg tgggcacggt gatcagcagc agtgccacgg
    13081 cccagaacag ctggcggcgc tccaggcgcc gcagcagggc cacaccgccc agcagcaacg
    13141 gcatgaggaa atagaaccac tcgtccagcg ccagcgacca ggcctcggca aagaaatacg
    13201 gcttggtctg ggcccaggcg aggttctggg aaaacacggc aaacgaccag gcaaagcgcc
    13261 cctcctcgat gccccaccag gcgatcaggg cattgatcag caggaacagg tagtagttgg
    13321 ggatggtgcg aaaccagcga cgcagccaga aatcgcgcgc gctggcccac cagccgccgc
    13381 gggtttcaaa gctgcggatc atcatcgagc cgatcaggta gccgctgagc acgaacagcc
    13441 actcgatgga aaacaccgcg caggcgctga gggcggcgcg ccagccctcc cacttcgaca
    13501 ggccggtact gacgaagaac cccaggtagc cgtggttcat cagcaccaca ccgatggcga
    13561 ccgaacgcag caggtcgaga ccgaagttgc gctggtagcg cggggcggaa ggtgtcgagc
    13621 tcatgaacgg tgggcgcggt cggtggcgct ggccggcgcg ctttcgctgg cgcgccaggc
    13681 gcgccacgcg ggcgagcgcc agagccggat caggctgcga gcatgccaca gcaggtggcg
    13741 cagacggcgt cgactggcgc gttgcgcctg gtgctcgacc gtcgccgcgt cggcgcgccg
    13801 cagccgccag ccggccagtt gcaggcgcag gcagaagtcc acgtcctcgc agtacatgtg
    13861 aaacgcctcg tcgaagcccc cgatggcctc gaaggccggc cggcgcagca gcaagaaggc
    13921 ggcgttgacc cagtcgggcg cctgaccggc gccgacctcc atgcgccgac ccagcagatg
    13981 ccggcgcagc agccgcagcg gcgtggggcg caggcgctca tggtcctgac ggcaaccgcg
    14041 cgcgtcggtc tgcaccgggt aggccgcgcc gacgcgggga tcgctttgca gctcggccac
    14101 cagcggcgga aacgggttga ccaggcagcg caggtcgggg ttgagcaccg cgaacagcgg
    14161 ctggccgcca tcgatgcgcg cgtcgagcgc aaaggcgcgg ttgtggttgg ccccaaaacc
    14221 cagcggtgcc gggttgtcga cgaggtgcag ctggaacggc cagtggcgcc cacgcaaccg
    14281 ctcggccgac gccggctcgg ggacgttgaa ggtgacgatc acgcgcgcca gcccgggctc
    14341 acccagcgcc gcgagctgcg ccagcagcgc ctcgaccgcg gcgccatgac cgtggctgac
    14401 gatggaggcg gcgatgttca ccagagatcc cggggctgcc tttcaatcca ccagttccgc
    14461 ccgcgacagg ggctgcccgg cccgatcctt ggccgacagc agcggcgcgg tgatgccgtg
    14521 cgcggccagc ggccagtcga tgccgatggc ggggtcgtcc caggcgatgc tgcgctcgtg
    14581 ctcgggggcg tagtagtcgg tggtcttgta caggaagtcg gcgctgtcgc tcagcaccac
    14641 gaagccgtgc gccaggccgg gcggcaccca aagctggcgg tggttgtcgg cgctgagctc
    14701 gacgccgacc cagcggccga acgtggctga gccgcggcgg atgtccaccg cgacgtcgaa
    14761 cacggcaccg tgggtcaccc gcaccagctt gccctgcggc tggcgcagct ggtagtgcag
    14821 gccacgcagc acgcccttgc ggctgcggct gtggttgtcc tgcacgaagt cgatgtccag
    14881 gccggtcgcc tgggcgaagc tgcggcggtt gtagctctcc atgaaaaaac cacggtcgtc
    14941 gccgaacact cggggttcga gcaccaggac gccgacaagt gcggtgggta tgacgtgcat
    15001 aggggttctc caggacggca gcgcagtcgg ttcagtcgcg gctgacgcgt atgccgtgcg
    15061 gcaggtagcg ccggtagcgc tgcagggcct gcgtcagtgc gtcggcggcc agttcgtaat
    15121 gaaagtgccg atcgtcggcg ccgatacggg tcgcgccgaa atgttcgtga aaccgccaga
    15181 cgctgagatt gtcctgcctg acttcgaaat aggccgagcg aaatcccagc cacaggccat
    15241 agtggtacgt catgagcgcc gactcgatgg cgcagtgaga cggcagcccg gccttgagca
    15301 gccaggagcc ccagctgaag gcgtcgcccc gcgcgccgta caggcgcacc gtgcccaggg
    15361 gctcgcccgc cgcgccatcg gcgatgacga aatacgcctg actcgaatcg gcggcatagc
    15421 ggcgcagcca ctcggtttgc gccgacggat cgggcggcac ggccgacaga aagcgcgcgc
    15481 gcacggggtc ggtgcgcagg gcatggatga aggcggcgtc ggactcgccc gcgtcgcgca
    15541 aggccagcgt gcgtccgtcg atgcgccggg ccttgcggat cgacggcggc atgggggctg
    15601 gtgggtgcat cacggctggc tcgccagacg acaggtgacg cgcagctcgc gcagccggaa
    15661 gctgccctgg ccatccaccg gatccaggcg cagacggggt tcgaacccca ccgggctgtc
    15721 gatggaaagt tccagccgcg catgcccatc cacgcccggc tggtaggcct ggcgcacgga
    15781 gcgcgcctcg ctgtcgctct gctggcccga ccatcggtag tacagctgtg cggtttcggc
    15841 gtgcgcggtg acgatgtcca gttgcacacc cagcgtgcgg cagcgccgcg acaaagcagc
    15901 gtcgggccat gcgaccgtga gctgggcgtc gccctcgctc tgcagcccgc catcggcccg
    15961 tcgccggaca tgggcccatg gcccgggctc cgtccccgcc gccgcgtgcc agtccagcaa
    16021 ctcacggcca aaaccgtcgc gcgtctgcac cggcaggtaa cgcacgaaag aggcctggat
    16081 gtcgcgcgcg tggatgtagg cgcgggtcag gtcggtcatg cccatcatgt cgtagccggg
    16141 gcagcgaatg ggctgcccgc tgccgagctg acgccccaga cagcgaagct cactggcgcg
    16201 gtacgccgag gccgatcggt agaacgatgg cacgtcgaac acaccgcgca ctgcggccag
    16261 cgccagcgcc gtcagcaatg tcccgaccag gaccggcacc gcccgtgtcc gccgccgggg
    16321 aaccagggcg gcggcgaccc acggaaacag cagcgtgacc cagaaaaagt gaaagcgctc
    16381 atagccgaac cggaacaccc cgcctgcgcc ctggatgcct gggtcgcgaa acccggcccg
    16441 gcccaggctg accagcacca gataggtcag cacgattgcg cacaagggca ggaacaccac
    16501 ggcgacgcga cgccgcggaa cgctcggcac gcggccgggc gccagcgctg cgcgccctgt
    16561 caggcccagc gccgccagca gggtcagcag caccagcgcg acccagatca gctccagcgg
    16621 gagcgacgcg aaaccatgcc cggtggcccg cccgatcttg cccgccagga atgcccagaa
    16681 gtcgggcgaa tcggggtaag tcaggcccat gtactgccga tccctggcct gcgcatcgcc
    16741 acgcgtcaga tggaactgca tggcgctggt caacccgccc gccagcagca gcgcgccacc
    16801 ggggccggcg atgcgggtgg ccagcgccgg cgtcgggtgc gtgcgcaaca cccgcgccag
    16861 caccagccag cacgcgccca gcaccagggc cgcgatggcg ccggaaatgt aggacaggcc
    16921 ggccaccgca cccaggacga acacccccag ggcggcgcgg cgccgcggcc gtggcaacgc
    16981 ccacgccatg gcacccagca gcgccagcaa gggcaaggcc tggtgatagg ccaggctctg
    17041 ttcgccccag tagctgcccg attgcagcat gggaacacag aaggcgaaca gcaatgcctg
    17101 ctggcgtcgg tcccggaccg cccagcccaa cagccgccac tgcagccaca gcaacccacc
    17161 cagcaccgcc agcatggaca gcgcctggta gggcaagggg ttgccaccca gccagcgctg
    17221 cgccagcacg tccagggtca ggccgaccgg cgagatggtg ttgttgacgg cgcgaaacag
    17281 atgccgcgga tcgagcgaca gggcgctgcc gtcgtcgtag acgcgccagt cgtccaggta
    17341 cggcaggtcg gtgccccagc gcagccaggc cagcaggttg gcgcccaaca tcagcagcgg
    17401 cagcgccatg ccggcgcgca gccaccactt cgacagcgcc ggggacaggg gttcagggcg
    17461 cggcatgcgg ggtgaagatc cagcgctgca gaacaaagat gaccggcaca cacaccagcg
    17521 ccgccgccag cggcgccagc caggccggac cgcgcagcca ggccacccag gcctcgacca
    17581 ccaccaggcc caggcccagt tgcacgccgt ggctggcggt gacatagacc cacgaaaagc
    17641 gcttgcccgg gcgcgcgaac acgacatgcc gcaacagcac gaaggacagc gcaacaccgg
    17701 ccaggaacgc cagcacatag gcccagcggt agtccagcaa tcgcagcagc gccaggtaca
    17761 gcaggtaggt cagcgcggtg ttggccccac ccgccagcag gtagcgcagg gcttcgcgcg
    17821 ccatcacgca gcgcctcccg cgcgtcgcag tgccaggaat tcgtcgtagt cgcggatgta
    17881 gtcggccgga tcgtacgggt ccgacgcaag caccagcagc acggcgccct gcgtgtacct
    17941 gtgctgaatg ccccaggtca tgggcggcag gtacaggccc aggctgggcc ggtcgaggca
    18001 gaactcttca cgctgccggc catcgtcggc gatgacgtgg accgaaccgg cgacggcctg
    18061 caggaactgg ccgcaccggc ggtgcgcgtg ggcgccgcgc agttcggcgc tgggtaccct
    18121 gtagacgagg aaactgcgac gcacggcaaa cggcagatcg gcgccgacct cggcgacgca
    18181 caggtcgccg cgtgcgtcct tcacctcgcg cagttcgtgc aggcgcaccc ccgtgacgcg
    18241 gctgtcgatg acgcgcggcg cgctggggac ggatgacgct ggcatgacgt gcggctagtg
    18301 ggactggaag cgatcgaccc gcaaggcgac ggcctgcggg cgctgcttgg tattttcgta
    18361 ggcgcgccag gcgtaggtgc cgacgatgcc caggctgaac aggttgagcg caccgaagaa
    18421 caggatggcg atcagcgtca tggcgtagcc aggcacaccg atcagcccgg aaagcttgcc
    18481 gaacagcacc accgcaccca gcacgccggc caccagcagg cccagcgccc ccacgcgcgt
    18541 gagcaggcgc accggcaggt cggtgaaggc gaacacgctg tcggccaggt aggccagctt
    18601 cttgcgccag gtccaggccg actggccgtg acggcgcggc tggcgctcgt aaggcacgct
    18661 ggcgcgccga aagcccagcc agaagatctg cgcgatcagc gagctgcgac tttcacccaa
    18721 gccgagcagg gcgtcgcgaa aggcgcggtt gcaggcaaag atgtcgacgc cgccgggcgg
    18781 catctcgggc accacccaac gccggtaggc ggcccagaac agctgggcct gccagcgctg
    18841 cagcagcggg tcgtggcgca gggtgcgctc ggccaccacc acgtcggcct ggccgcgcgc
    18901 cagctcggcc tgcatctgca acaccagctc gggcggctcc tgcaggtcgg cggccatcac
    18961 ggcaaaggtg tcgccgcgcg cctgctccag gcccatgcgc acggcggcaa aggcgccgaa
    19021 attgcgcgtg agcagcacca gggtggcgtt caggccgctg gcgggcagct gctggcgcag
    19081 cagcgcgtgg cagcggtcgg ggctgccgtc gaccacgaac accacctcca gcgcgccggt
    19141 cagcgcctgg tgcaacccct gcagctcggt cagcagctcc ggaatcgaag cctcgttgcg
    19201 gtagaccggg atgatcaggc tgagcatggt caggcagccg cgaagaattg gcgcacgacc
    19261 tcgatgacac gcgcgccctg cggcgctgtc agtccagggt agcagggcag cgacagcacc
    19321 tcggcacagg ccgcctcggt gaccggcagg tgcggcagcg gctgggcggc acgcaccggc
    19381 tgacggtggt cggccagcgg gtagtgaatg tcgctggcca cgccgtgcgc cgccagatga
    19441 gcctgcaggg cggcgcggtg cggggtgcgc acgacgtaca ggtgcatcgc gtggtccggc
    19501 ccaggcggcg gcgggcagtc cagcggcaag ccggcgaagg ccgcgctgta gccggcggcg
    19561 atggcgcggc gctcggcatt ggcggcctcc aggtggggca gcttggccag cagcaccgcg
    19621 gcctgcagct cgtccagacg gctgttgcca ccaccgggca gaccgacttc gtacttgcgt
    19681 gtccagccgt actggcgcag ggcgcgcagg cgttcggcca gcgcggcgtc gtcggtcaac
    19741 acggcgccgc cgtcgcccag ggcgcccagg ttcttggtgg ggtagaagct gaaacaggcc
    19801 agcgtgcccc aggcgccggc gcagcgcccg tgcagcgcgg cgccatgcgc ctgcgcgcag
    19861 tcttcgatca gcggtacgcc ggcagcctgg cacagcgccg tcaggcgcgg catgtcggcc
    19921 atctggccgt acaggtgggt gacgatgaca gccgccacgc ggccctccgc cagcgcagcg
    19981 gccagcgcct cgggcgccat cgtcaggcgc gcgggatcga catcgaccca gccgggatcg
    20041 gcccccacct gggccatggc ggtgctgctg taacaccctg cattggccac ggtgagcacg
    20101 cgctggccgg gttgtatgcc cagggcgcgc agggccagcg tcagggcgtc ggtgccgttg
    20161 gccacgccca ccgcgtgccg cacgccgaca taccgggcaa acgccgtctc gaattcgcgc
    20221 acctgcggcc ccagcacgta ccagccgctg tcgaccacgc gctgcagaca ggcgcccaga
    20281 tcgacgccga aggcggcccc gaacggggag aagaacgcca cctgcggcgc gctggccatg
    20341 tcagcgccgc tccgccggct gggccggctc ctgcagcaag cgccgcaggt actggccgta
    20401 gccgctcttg gccagcggcg ccgccaggcg ctcgagctgg gcgtcgtcga tgaagcgctt
    20461 gcgccaggcg acttcttccg ggcaggcgat cttcaggccc tggcgccgct ccagggtggc
    20521 gatgaactgg ccggcatcga gcaggctgtc gtgggtgccg gtgtccagcc
//
LOCUS       DAOOOG010000029        19386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_2841_length_19386_cov_27.704930, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000029 DAOOOG010000000
VERSION     DAOOOG010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 19386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19386
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2841_length_19386_cov_27.704930"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PK242_03970"
     CDS             complement(<1..242)
                     /locus_tag="PK242_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ93206.1"
                     /translation="MTTPTGFLTGKKLLITGVLSNRSIAYGIARACRAQGAELAFSYV
                     GERFKDRITEFAAEFDSRLVFDCDVGSDEQIERMFR"
     gene            280..2073
                     /locus_tag="PK242_03975"
     CDS             280..2073
                     /locus_tag="PK242_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOZ93207.1"
                     /translation="MRIFSAVLLAAFSLPAWAAHGYALWGELKYPPGFAHFDYVNPAA
                     PKGGEIRLVGNSRASTFDKYNPFTIKGSAPTYLADLLFDSLLAGSLDEAGAAYGLLAE
                     DVSVAPDRLSATFRLRAQARFHNGDPVLARDVQHSFQTLIGPHTSPAYKTLLAEVAGV
                     DVLDERTVRFRFRQPNRELPLTVGGLPVFSHQWGAGKPFDQVVMDEPIGSGPYRIGPV
                     KFGKDITYVRDARYWARDLNVRRGTGNFDRVQVRIYRDNTARLEALKAGEFDLMAFYS
                     AGDWARRVNGPKFRSGELVKGEFRHRLPTGFQSYVLNSRRPLLHDVRVRQALGLAIDY
                     EWMNRQMFYQSYTRVRGIFGNTECAASGMPDEAQLALMTPFRADIPPAAFGPAFEPPR
                     TDGSDNGLRENLRQAQRLLAEAGWRVQGGVLTNAQGQPLVLEYLDSNEGGARVVTPWM
                     RNLEKLGIRLNFRPVDFALYQQRLQKFDFDITTIAYQGTLNPGQEYADLFGSRAADTE
                     DSGNLAGVKNPAIDSLISHMVGARAHPEFLAACRALERVVSHSHYLLPQWYSPVHRLA
                     YNAWRLDKPAVVPPYFRGEAWAIDTWWARRR"
     assembly_gap    2079..2178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2352..3290)
                     /locus_tag="PK242_03980"
     CDS             complement(2352..3290)
                     /locus_tag="PK242_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HOZ93208.1"
                     /translation="MSDTATASFTASALHGIAPLPHLGVIRAHGEDAAHFLQGQLTQD
                     VTGLKPGQARLAALCNPKGRMLASFQVLRRADDELLLVTSADTLAATLKRLSMFVLRA
                     RLKLSDATAELALRGLLGNATTSVAAGAHQTSAGAQNDPAFTIALPAADGAPRALWLA
                     AAGTPPPAGAELSEADWLAAEVRAGVARITRPVVEAFVPQMLNYESVDGVHFQKGCYP
                     GQEVVARSQFRGAIKRRAFVGWADGAVQAGQEVFSASAPEQPAGLIAQAAAVAGGTAV
                     IAALRLDALPTALAVGHAAGVPLRDLHIPYPLREDL"
     gene            3338..4336
                     /gene="mltG"
                     /locus_tag="PK242_03985"
     CDS             3338..4336
                     /gene="mltG"
                     /locus_tag="PK242_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOZ93209.1"
                     /translation="MRTLLKLLALLLALALAAGAGAWWWTQQPLPLAADALEISVPEG
                     ASLRTAAQRAVDGGVQTPAALLAGYFRLAARGQTIKPGDYALTRGMTAAELLGKLVRG
                     ERIVLTVKLLEGWTFRQLRQALAQAPRLRHDTQDMTPAQIMQALGRPGVAPEGRFFPD
                     TFHYFRNASELDILRQSMQLMEQRLQAAWDARAPDLPLKSADEALVLASIVEKETGRA
                     QDRAEIAGVFINRLRIGMRLQTDPSVIYGLGEGFDGDLKRSHLSADTPYNTYTRAGLP
                     PTPIAMPGKAALLAAVQPAATQALYFVARGDGSSHFSRTLDEHNRAVQRYQRGGSR"
     gene            4336..4992
                     /gene="tmk"
                     /locus_tag="PK242_03990"
     CDS             4336..4992
                     /gene="tmk"
                     /locus_tag="PK242_03990"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOZ93210.1"
                     /translation="MAQHGRFISFEGIDGAGKSTHIEALAQALREAGRTVVRTREPGG
                     TPLAEKIRALALHEAMDALTETLLMFAARRDHLTQVIVPALARGEVVLCDRFTDATFA
                     YQGGGRGFDWESLSLLERLAQTVQAPGADLAHEGMVQPDLTIWFDLPPAVAAERLAGT
                     RVPDRFEAQPAAFFEAVRAGYARRASQQPARFARIDADQPREAVWRAVHGAVQARGWL
                     "
     gene            4989..5993
                     /locus_tag="PK242_03995"
     CDS             4989..5993
                     /locus_tag="PK242_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOZ93211.1"
                     /translation="MSGAALAPWIARQHATLRAQRGHAWLLQGPSGLGQFELALALVR
                     AWLCDQPTPDGACGLCPSCHGIDVHTHPDLVVLMPETEMLARGWPLPEKAQAEIDDKK
                     RKPSREIRVDAMREAIEFAQRTSGRGRGKAVLVYPAERMNAVTANALLKTLEEPPGDA
                     RFVLATDAAHLLLPTIRSRCLSHTLAWPPADEALAWLATQGLAGPQAAALLQAAGGRP
                     AEALALAEGKDSARRWSQLAPALARGDVAALADLPLPEAVARLQKLCHDLLALQVGAA
                     PRFFAPADLPRQPLSLLALTRWWQQLAQSARTAEHPLNAGLATEFLVSSARQALNSGR
                     "
     gene            6022..6393
                     /locus_tag="PK242_04000"
     CDS             6022..6393
                     /locus_tag="PK242_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOZ93212.1"
                     /translation="MSTTPNAAASTPRPSVIQLAIKEKAALYAAYIPMFAEGGIFIPS
                     TRDYRLGDDVYVLITLPDDPQRFPIAGKVAWITPARSVGNRTQGIGIRFPKDEKSAQL
                     KTKIEQILGTALGSDRPTQTI"
     gene            6472..7278
                     /locus_tag="PK242_04005"
     CDS             6472..7278
                     /locus_tag="PK242_04005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOZ93213.1"
                     /translation="MFTDSHCHLTFPELAGDIPAVRAAMAAARVTRALCICTTMEEFP
                     AVQALASGHDNFWATVGVHPDSEQVHEPDVEELVAATTRPRVVAVGETGLDYYQMDER
                     KGGRSIADMGWQRERFRTHIRAARRARVPLVIHTRAAADDTLAILREEGEDGSAGSAG
                     GVFHCFTETRAVARAALDMGFYVSLSGIVTFRNARELHEVATLIPDDRLLIETDAPYL
                     APVPHRGKLNTPAYVPLVAARLAELRGTRTERIGELTSANFTRLFSRVAP"
     gene            7284..7952
                     /locus_tag="PK242_04010"
     CDS             7284..7952
                     /locus_tag="PK242_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOZ93214.1"
                     /translation="MLRRLFLTALLGLCSAAALADAHSDEFFRAIRQDNDAAVRSLLA
                     RGFDPNTKDEKGTPGLVMALQEGSLRVADLLIEHPRLVPESRNAADESPLMMAALKGH
                     TELVRRLIARDADVNKPGWAPLHYAATGGHLDIMRQLLEAHAFIDAESPNGTTPLMMA
                     AFYGTPEAVKLLIEAGADIGMRNQKGMTALDFAQRAERQNSIELLQTALRWKLRGGAP
                     RGQW"
     gene            complement(7972..9234)
                     /locus_tag="PK242_04015"
     CDS             complement(7972..9234)
                     /locus_tag="PK242_04015"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HOZ93215.1"
                     /translation="MLARLWAAPLLCCGFRPFFLGAALWAALAMGLWMLMLTTTLPLV
                     PGPLSGSAWHAHEMVWGFGMAAVTGFLLTAVPEFTATPEFKRRDTALLFGLWLAARLF
                     GLVGLALLPALWVPLTAALNLALVASLAWRSLPRLLRQPLRPHQGFALALLALLGVEA
                     GFWWAALGGRDAMPWVNACVGLMMVLIVLAQSRISMRLLRGMLDHLGVHQADYRAPPP
                     RRRLAIFCIALLTGTELLGVAEPTLGWLALAASAAVLALMSEWHFGRALGHRWVWPLY
                     AVYLCMAAGYALLGLAWLGAPWAPSAGRHALTVGSMGLGVLAVMNIAGRIHAGLPLDD
                     RRWRVVASALLVAAVGARVLASTPWAAGGTLAAWWLAALAWAVAWLLYAGFAWRHLSG
                     PRTDGGTGCEEPLDAAAPAALRPPLAAD"
     gene            complement(9260..10090)
                     /locus_tag="PK242_04020"
     CDS             complement(9260..10090)
                     /locus_tag="PK242_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ93216.1"
                     /translation="MTALALRGVGHRFGPETVLEGIDLQIAPGEVLALLGRSGCGKTT
                     LLRIAAGLLTPTEGQMQTDFSRSAIVFQQPSLLPWKRLLDNIALGLKARGLPRSECHA
                     RAAALAAQVGLRPEDHAKFPAELSGGMQSRAALARAFAVDPALLLLDEPFSALDVGLR
                     AEMHALLARQVGERGCAALLVTHDLMDAVRLAQQLVLLRPDPGRVLARFHLDRPLAAR
                     DDAWVYRQTARLLAHRLVREAFNLPPPVDAPAALPAHEALRASPHPDIPQQVRRHGCG
                     "
     gene            complement(10087..10920)
                     /locus_tag="PK242_04025"
     CDS             complement(10087..10920)
                     /locus_tag="PK242_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOZ93217.1"
                     /translation="MADRRTAADLTDTRRARWPARVGHYLWSGWTGAASVLLLVAAWD
                     ALARQLGPLVLPTPQETLASLTQILRSPAAWLDLGATARRALYGYGLSVLAGAVLGMA
                     AGASITATMMARPLVTLLIGMPPIAWLVLALLWFGSGDATPVFTVFVACFPIIFVGAL
                     QGTRTLDGRLRELAQAYRLPLTQRFADVYLPHIASYLFAACITALGVSWKVVLMAELL
                     ATSDGAGAQLAATRSHLDMGGTLAWVSAVVGLLLAAEYLLLEPIRREMERWRADGGGA
                     A"
     gene            complement(10930..11931)
                     /locus_tag="PK242_04030"
     CDS             complement(10930..11931)
                     /locus_tag="PK242_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ93218.1"
                     /translation="MTCRRRFLQCTALLGAAPGALLGARAATLPELRLSGPPASVSNP
                     LIHLAAQGRVPGLAERVRYLPWRDPDQLRALALRGEADFVAMPTNVAANLYNRGVPLK
                     LVNVSVWGILFLVSRDAERRTLADFRGEEIAMPFREDMPDLVFQEAAQALGLDPKKDF
                     RLRYVASPLDAMQLLITRRVRHALLAEPAVSMALRKTQSFPVSAIAPELHRAVDIQQE
                     WARAFRRRPRIAQAGIVALGAAAQDAALIARFRDAYAGATAWCQSQRAACGAEVAAVE
                     SRLSAEAVADSLGAAPLDAVPAAQARDELLFLFERLFKRHPQLLGGKLPDAGFLAGA"
     gene            complement(11937..14054)
                     /locus_tag="PK242_04035"
     CDS             complement(11937..14054)
                     /locus_tag="PK242_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ93219.1"
                     /translation="MKLPTACPTPTDASRAKLTFRRPRGPLPNLRPTPLALALLGLCA
                     SAQAQTAAPAPADNQLPEIAVTAKGYAADTLDTPASIDVIDAQEIRRKGATSLGQALQ
                     GEPGLAATSDGSASLNPVVRGLKKESLALMVDGMRLNSAQPYGAIGSFVSLGLAERVE
                     VLRGPASVLYGSGALGGAINVLLPQARFDAGVSGRLGLRAASGDRSVGASGVVNWSGG
                     DHALMLGAAGADHGNYRAGGQRVERTGYEQHALIGQYRYRIDGAQQIRLSLQRENLND
                     VWYPGSTQPNANPMVGSTTIHSPRQSRQLMEIGYSHKGRGPVNFDARLYRQSVDRTIN
                     AWANGPLQRDTTTNDVAFDTTGLDVRADWAANEHHLISVGFNGWRMTGSPDRRIFNNG
                     SYVRNNPFEDGWVQAAGLYVQDDMRFGALGVVAGLRYDRVQGNAASVANGSVTTGLKR
                     GDGAVSGNLGLIYELDPLLRPYASLSRGFRPGEMRERFEASPRGDGYFYLGNPQIRPE
                     IAQQIEIGVKGKSEQLEWFAAVYRNRISDYITGRATGQTQNGTPVRQTINLGSVRISG
                     LELGARWQFARGHWLGLGYSRLRGRNNDFNEPLYQMPADELNLSWEGRLGAGWSADAR
                     VRHAWRQDRVATVFARGLEDATPGFTTLDIGATWARGPHQLRLAVTNLTDRKYHEHLA
                     EGLAGREVPAPGRSLVVSYQLQF"
     gene            14449..16281
                     /locus_tag="PK242_04040"
     CDS             14449..16281
                     /locus_tag="PK242_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOZ93220.1"
                     /translation="MSTLRNTLDFLLYDWLAADRLTERPRFADHSRETFDAVLDTCER
                     IAREKYAPFNRLVDTEEPRTETAPDGSLRVVLPRATHEARTAYAESGMLAAAQDHEHG
                     GMQLPYLVEAAANAFFGKASISIGGNLLTVGNANLLMAHGTPLQKQVFAANEFNGRWA
                     GTMCLSEPQAGSSLSDIATRAELEDASDPLGPRYRLRGNKMWISAGEHEMTENIVHLV
                     LAKIPGADGKLVPGVKGISLFIVPKRLVDTEGRLTGVRNDVALAGLNHKLGWRGTTNT
                     LLNFGEGKFPVDGQPGAVGYLVGQPGEGLRCMFHMMNEARIGIGMAATMLGLAGYEAS
                     LDYAKTRIQGRPIGGSGKDAAQPPVRLIEHADIRRMLLAQKSWCEGALALLLYCARLV
                     DEQHTGAPEAADEARLLLEVLTPVAKSWPSENCLEANSLAIQIHGGYGYTRDFPVEQY
                     WRDNRLNMIHEGTHGIQAMDLLGRKVRMEQGRGLALLAARVQATIARAQTHAALQPDA
                     AALGAALDRIQQATRAAWADADANAALANAVPYMQAFGHVVIAWIWLELSLASSTTKT
                     IAAGAGVAGARTYFYRYELPKIDAWLRVVESRDLTCAQLPEEAF"
     gene            16281..16583
                     /locus_tag="PK242_04045"
     CDS             16281..16583
                     /locus_tag="PK242_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOZ93221.1"
                     /translation="MLKHIVMWKFKDQAEGADKAANLAKAVALLEGCADLVPGTLRLE
                     VATAQPGLEATYDLVLYSEFEGRAALDAYQNHPTHVAVKPFLRAVVSERQCMDYEL"
     gene            16604..17398
                     /locus_tag="PK242_04050"
     CDS             16604..17398
                     /locus_tag="PK242_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ93222.1"
                     /translation="MTRTVQQLFDLKGKTALITGGSRGLGLQMAHALGEAGARLMLSS
                     RKAGDLEQAVAELQAAGIDARWIAADCSKEEDTRRLADETLERMGAIDILVNNAGASW
                     GAAAEEHPVAAWDKVMNLNVRGYFILSQQVANGWMIPNKAGRIINIASIAGLNGNPPE
                     MKTLAYNTSKTAVIGFTRTLAAEWGRFGITVNAICPGFFRTKMANGLIDQLGEGKMAA
                     AAPLGRLGDEEDLKGITLLYASDAGKHITGQWLAVDGGVSVVLGAA"
     gene            17524..17958
                     /locus_tag="PK242_04055"
     CDS             17524..17958
                     /locus_tag="PK242_04055"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOZ93223.1"
                     /translation="MSLDFGAHIPFVHHLGFTLERFEGGASTLHFAPRAEHCNSFGVA
                     HGGALMTFLDVVMATAARSANPGSGIVTIEMKTSFMRPAPAAGGVLVGQGRLAHRTRS
                     MAFVEGSVFDAEGQLCAQSTGTFKYVPRAQPTGQPAGPLPTD"
     gene            18047..19063
                     /locus_tag="PK242_04060"
     CDS             18047..19063
                     /locus_tag="PK242_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HOZ93224.1"
                     /translation="MPTNRQILLDNRPQGEATASNFRLVSSETPALRDGQVLVRHHYL
                     SLDPYMRGRMNDGKSYAQPQPLGEVMIGGTVGEVLQSRHPRFQPGDAVVGMGGWQEYS
                     VVDADQPGLLRKVDTTHVPLSYYLGAVGMPGVTAWYGLTQIIAPQAGETVVISAASGA
                     VGSAFAAVAKARGCRVVGIAGGPEKCRYAQEELHFDACIDYRQHPDIKSMAQALKAAC
                     PQGVDGYFENVGGYLLDAVLLRTNAFARIAVCGMIAGYDGQPLPLQNPALILINRLKV
                     QGFIVSEHMDLWPQALQELGSLVAQGRLRPRESIALGIESAPEAFLGLLKGRNFGKQL
                     VKLI"
     gene            19092..>19386
                     /locus_tag="PK242_04065"
     CDS             19092..>19386
                     /locus_tag="PK242_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation response protein"
                     /protein_id="HOZ93225.1"
                     /translation="MSTVTHEVLNQPTPLQDINLFEGHQALRDALRLHAPGLDTASLA
                     SLGKLAGSAEMQRHARLANVHTPELRTHDRFGHRVDEVEFHPSYHALMSAATAA"
BASE COUNT         2971 a         6907 c         6784 g         2624 t
ORIGIN      
        1 tcgcggaaca tgcgctcgat ctgctcgtcg ctgccgacgt cgcagtcgaa caccagcctg
       61 gagtcgaatt cggcggcgaa ctcggtgata cggtccttga agcgctcgcc gacgtagctg
      121 aaggccagct cggccccctg tgcgcggcag gcgcgggcaa tgccgtaggc gatggaccgg
      181 ttggacagca cgccggtgat cagcagtttc ttgcccgtca gaaagccggt gggggtggtc
      241 atgcgctagc tcctggtaaa tcagcggcag aattgtcgca tgcgcatttt ctccgccgtg
      301 ctgctggcgg ccttcagtct gcccgcctgg gccgcccacg gctacgcgct gtggggcgag
      361 ctgaagtacc cgcccggctt tgcccatttc gactacgtca acccggccgc gcccaagggc
      421 ggcgaaatcc gtctggtggg caattcgcgc gcctccacct tcgacaagta caaccccttc
      481 accatcaagg gcagcgcgcc gacctacctg gccgatctgc tgttcgacag cctgctggcc
      541 ggctcgctgg acgaagccgg cgctgcctac ggcctgctgg ccgaggacgt cagcgtggcg
      601 cccgaccgcc tgtcggccac cttccgcctg cgcgcccagg cgcgctttca caacggcgac
      661 ccggtgctgg cgcgcgacgt gcagcacagc tttcagaccc tgatcggccc gcacacttcg
      721 cccgcctaca agacgctgct ggccgaggtg gcgggggtgg acgtgctgga cgagcgcacc
      781 gtgcgctttc gctttcgcca gcccaaccgc gaactgccgc tgacggtggg cggcctgccg
      841 gtgttcagcc accaatgggg cgccggcaag cccttcgacc aggtggtgat ggacgagccc
      901 atcggcagcg ggccctaccg catcgggccg gtgaagttcg gcaaggacat cacctacgtg
      961 cgcgacgccc gctattgggc gcgcgacctg aacgtgcgcc gcggcaccgg caacttcgac
     1021 cgcgtgcagg tcaggatcta ccgcgacaac accgcccggc tggaggcgct gaaggcgggc
     1081 gaattcgacc tcatggcgtt ctattcggcc ggcgactggg cgcggcgcgt caacgggccg
     1141 aagttccgca gcggcgagct ggtcaagggc gagttccgcc accggctgcc caccggcttt
     1201 caaagctacg tgctcaacag ccgccggccg ctgctgcacg acgtgcgcgt gcgccaggcg
     1261 ctgggcctgg cgatcgacta cgaatggatg aaccggcaga tgttctacca gtcgtacacg
     1321 cgggtgcgcg gcatcttcgg caacaccgaa tgcgccgcca gcggcatgcc cgacgaggcc
     1381 cagctggcgc tgatgacccc gtttcgcgcc gacatcccgc cggcggcctt cggcccggcc
     1441 ttcgagccgc cgcgcaccga cggcagcgac aacggcctgc gcgagaacct gcgccaggcc
     1501 cagcgcctgc tggccgaggc cggctggcgc gtgcagggcg gcgtgctgac caacgcccag
     1561 ggccagcccc tggtgctgga gtacctggac agcaacgaag gcggcgcgcg cgtggtcacg
     1621 ccctggatgc gcaacctcga aaagctgggc atccgcctga acttccgccc ggtggacttc
     1681 gcgctgtacc agcagcgctt gcagaagttc gacttcgaca tcaccaccat cgcctaccag
     1741 ggcacgctca acccgggaca ggaatacgcc gacctgtttg gcagccgcgc cgccgacacc
     1801 gaggattcag gcaacctggc cggcgtgaag aaccccgcca tcgacagcct gatcagccac
     1861 atggtgggcg cgcgcgcgca ccccgagttc ctggccgcct gccgcgcgct ggagcgcgtg
     1921 gtcagccaca gccactacct gctgccgcag tggtattcac ccgtgcaccg gctggcctac
     1981 aacgcctggc ggctggacaa gccggccgtg gtgccgccgt acttccgcgg cgaggcctgg
     2041 gccatcgaca cctggtgggc gcgtcggcgt tgaaggcgnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnntc cgcggcgagg cctgggccat cgacacctgg tgggcgcgtc
     2221 ggcgttgaag gcggcgccgg gggtgggcgt acagcgcctt cacccggaag atgaaggctc
     2281 cgaagtggcg caaggtcgca gttcatgcgc cattcccaac ggttgcaggc ggtcaactgc
     2341 cgtttccagg ttcaaaggtc ctcgcgcagc gggtagggaa tgtgcaggtc gcgcagcggc
     2401 acgccggcgg cgtggccgac ggccagcgcg gtgggcagcg cgtccagccg cagcgcggcg
     2461 atcaccgccg tgccgccggc cacggcggcg gcctgggcga tcaggccggc cggctgctcg
     2521 ggcgcgctgg cgctgaacac ttcctgcccc gcctgcaccg cgccgtcggc ccagccgacg
     2581 aaggcgcgcc gcttgatggc gccgcgaaac tggctgcgcg ccaccacctc ctgcccgggg
     2641 tagcagcctt tctgaaaatg caccccgtcc acgctttcgt agttgagcat ctgcggcacg
     2701 aaggcctcga ccaccggccg cgtgatgcgc gccacgccgg cgcgcacctc ggcggccagc
     2761 cagtcggcct cgctgagttc ggctccggcc ggcggcggcg tgccggccgc cgccagccag
     2821 agcgcgcgcg gtgcgccgtc ggcggccggc agggcgatcg tgaatgctgg gtcgttttgg
     2881 gctccagcgc ttgtctgatg ggcgccagca gctactgaag tggtagcgtt tccgagcagg
     2941 ccgcgcaacg ccagttcggc cgtggcatcg ctcagcttca gcctggcgcg cagcacgaac
     3001 atgctcaggc gcttgagcgt ggccgccagc gtgtcggcgc tggtgaccag cagcagctcg
     3061 tcgtcggcgc ggcgcagcac ctgaaagctg gccagcatgc gccccttggg gttgcacagg
     3121 gccgccaggc gcgcctggcc cggcttcagc cccgtcacgt cctgcgtcag ctggccctgc
     3181 agaaagtgcg ccgcgtcttc cccgtgggcg cggatcacgc ccaggtgggg caggggagcg
     3241 atgccgtgca gggccgaggc ggtgaaggaa gcggttgcgg tgtccgacat gggctgaatt
     3301 atcatggccg gttcccattc acgccctgcg ccgactcgtg cgaaccctgc tgaaactgct
     3361 ggcattgctg ctggcgctgg ccctggcggc cggcgccggg gcctggtggt ggacgcagca
     3421 gccgctgccg ctggccgccg acgcgctgga aatcagcgtg cccgaaggcg cctcgctgcg
     3481 cacggcggcg cagcgcgcgg tggacggcgg cgtgcagacc ccggcggcgc tgctggcggg
     3541 ctacttccgg ctggcggcgc gcgggcagac catcaagccg ggcgactacg cgctgacgcg
     3601 cggcatgacg gcggccgagc tgctgggcaa gctggtgcgc ggcgaacgca tcgtgctcac
     3661 cgtcaagctg ctggagggct ggactttccg ccagttgcgt caggcgctgg cgcaggcgcc
     3721 gcggctgcgc cacgacaccc aggacatgac gccggcgcag atcatgcagg cgctgggccg
     3781 gcccggcgtg gcgccggagg ggcgtttctt tcccgacacc ttccattact ttcgcaacgc
     3841 cagcgagctg gacatcctgc gccagtccat gcagctgatg gagcagcgcc tgcaggccgc
     3901 ctgggacgcg cgcgcgcccg acctgccgct gaaaagcgcc gacgaggcgc tggtgctggc
     3961 cagcatcgtc gaaaaggaaa ccggccgcgc ccaggaccgg gccgagatcg ccggcgtctt
     4021 catcaaccgg ctgcgcatcg gcatgcggct gcagaccgac ccgagcgtga tctacggcct
     4081 gggcgagggg ttcgacggcg acctcaagcg cagccacctg agcgccgaca ccccctacaa
     4141 cacctacacc cgcgccggtc tgccgcccac accgatcgcc atgcccggca aggcggcgct
     4201 gctggcggcg gtgcagccgg cggccacgca ggcgctgtat ttcgtggcgc gtggcgacgg
     4261 cagcagccac ttcagccgca cgctggacga acacaaccgc gccgtccagc ggtaccagcg
     4321 cggggggagc cggtgatggc gcagcacggg cggttcatca gcttcgaagg catcgacggg
     4381 gccggcaagt cgacccacat cgaggcgctg gcccaggcgc tgcgcgaggc cgggcgcacc
     4441 gtggtgcgca cgcgcgaacc cggcggtacg ccgctggccg agaaaatccg cgcgctggcg
     4501 ctgcacgagg cgatggacgc gctgaccgaa acgctgctga tgttcgccgc ccggcgcgac
     4561 catctgaccc aggtgatcgt tccggcgctg gcgcgcggcg aggtggtgct gtgcgaccgc
     4621 ttcaccgacg ccaccttcgc ttaccagggc ggcgggcgcg ggttcgactg ggagtcgcta
     4681 tcgttattgg agcgtctggc gcagactgtg caagcgccag gggccgattt ggcccatgaa
     4741 ggaatggtcc agcctgacct caccatctgg ttcgatctgc cgcccgcggt ggcggccgaa
     4801 cggctggccg gcacccgcgt gcccgaccgc ttcgaggccc agccggccgc cttcttcgag
     4861 gccgtgcgtg ccggctacgc gcgccgcgcc agccagcagc cggcgcgctt tgcccgcatc
     4921 gacgccgacc agccgcgcga ggcggtgtgg cgtgccgtcc acggcgccgt gcaggcgcgg
     4981 ggttggctgt gagcggcgcc gccctggctc cctggatcgc gcgccagcac gcgacgctgc
     5041 gggcccagcg cggccacgcc tggctgctgc aggggccgtc gggcctgggc cagttcgagc
     5101 tggcgctggc gctggtgcgc gcctggctgt gcgaccagcc gacgccggac ggcgcctgcg
     5161 gcctgtgccc gagctgccac ggcatcgacg tgcacaccca ccccgacctg gtggtgctga
     5221 tgcccgaaac cgagatgctg gcgcgcggct ggccgctgcc cgagaaggcg caggccgaga
     5281 tcgacgacaa gaagcgcaag cccagccgcg agatccgcgt cgacgccatg cgcgaagcga
     5341 tcgaattcgc ccagcgcacc agcggccgtg gccggggcaa ggcggtgctg gtgtacccgg
     5401 ccgagcgcat gaacgcggtc accgccaacg cgctgctcaa gacgctggag gagccgccgg
     5461 gcgacgcccg cttcgtgctg gcgaccgacg ccgcgcacct gctgctgccc accatccgca
     5521 gccgttgcct gagccacacc ctggcctggc cgccggccga cgaggccctg gcctggctgg
     5581 ccacccaggg tctggcgggc ccgcaggccg ccgccctgct gcaggccgcc ggcggccgac
     5641 cggccgaggc gctggcgctg gccgaaggca aggacagcgc gcgccgctgg tcgcagctgg
     5701 cgccggcgct ggcgcgcggc gacgtggccg ccctggccga tttgccgctg ccggaggcgg
     5761 tggcgcggct gcagaagctg tgccatgacc tgctggcgct gcaggtgggc gcggcgccgc
     5821 gctttttcgc gccggccgat ctgccgcgcc agccgctgtc gctgctggcg ctgacgcgct
     5881 ggtggcagca gctggcgcag tcggcgcgca cggcggaaca cccattaaat gccggtttgg
     5941 cgacagaatt cctggtcagc agcgcccgtc aggccctaaa ctccggccgc tgacccttct
     6001 gtcgcgccca cgcccatcgc catgtcgacc accccgaatg cggcggcttc cacgccccgg
     6061 cccagcgtca tccagctggc gatcaaggaa aaggcggccc tgtacgccgc ctacattccc
     6121 atgttcgccg aaggcggcat cttcattcct tcgacgcgcg actaccggct gggcgacgac
     6181 gtctacgtgc tcatcaccct gccggacgac ccacagcgtt tcccgatcgc cggcaaggtg
     6241 gcctggatca cgcccgcgcg ctcggtcggc aaccgcaccc agggcatcgg catccgcttc
     6301 ccgaaagacg aaaaatcggc ccagctcaag accaagatcg aacagatcct gggtaccgcc
     6361 ctgggctccg accgccccac gcagaccatc tgaacgcccg ggttcctgcc gggctgctag
     6421 catcggcgcc gcgttctgat gcggcccctg gccgcaggcg cggccatccg catgttcacc
     6481 gattcccact gccacctgac ctttcccgaa ctggcgggtg acatccccgc cgtgcgcgcc
     6541 gccatggccg ccgcccgcgt gacgcgcgcg ctgtgcatct gcaccacgat ggaagagttc
     6601 cccgccgtgc aggcgctggc cagcggccac gacaacttct gggccacggt gggcgtgcat
     6661 cccgacagcg agcaggtgca tgaaccggac gtcgaggagc tggtggccgc caccacccgg
     6721 ccgcgcgtgg tggcggtggg cgaaaccggc ctcgattact accagatgga cgagcgcaag
     6781 ggcggccgca gcattgccga catgggctgg cagcgcgaac gctttcgcac ccacatccgc
     6841 gccgcgcggc gggcccgcgt gccgctggtg atccacaccc gcgccgccgc cgacgacacc
     6901 ctggccatcc tgcgcgaaga aggcgaggac ggcagcgccg gcagcgccgg cggggtgttt
     6961 cactgcttca ccgagacccg ggccgtggcg cgcgccgcgc tcgacatggg tttctacgtc
     7021 tcgctgtcgg gcatcgtcac cttcaggaat gcgcgcgagc tgcacgaggt ggcgaccctg
     7081 atccccgacg accgcctgct gatcgaaacc gacgcgccct atctggcgcc ggtgccgcac
     7141 cgcggcaagc tcaacacgcc ggcctacgtg cccctggtgg cggcgcggct ggccgagctg
     7201 cgcggcaccc gcaccgaacg catcggtgaa ctgaccagtg ccaacttcac ccgcctgttt
     7261 tcgagggttg ccccatgagc gccatgcttc gccgcctgtt cctgaccgcc ctgctgggcc
     7321 tgtgcagcgc cgccgcgctg gccgacgccc acagcgacga attctttcgc gccatccggc
     7381 aggacaacga cgccgccgtg cgcagcctgc tggcgcgcgg ctttgacccc aacaccaagg
     7441 acgaaaaagg cacgcccggg ctggtgatgg cgctgcagga aggctcgctg cgcgtggccg
     7501 acctgctgat cgagcacccg cgcctggtgc ccgagagccg caacgccgcc gacgaaagcc
     7561 cgctcatgat ggcggcgctg aaaggccaca ccgagctggt gcgccggctc atcgcgcgcg
     7621 atgccgacgt caacaagccc ggctgggcgc cgctgcacta cgccgccacc ggcgggcact
     7681 tggacatcat gcgccagttg ctggaagcgc atgccttcat cgacgccgaa tcgcccaacg
     7741 gcaccacgcc gctgatgatg gcggccttct acggtacgcc cgaggcggtc aagctgctga
     7801 tcgaggccgg cgccgacatc ggcatgcgca accagaaggg catgacggcg ctcgacttcg
     7861 cccagcgcgc cgagcgccag aactcgatcg agctgctgca gaccgcgctg cgctggaagc
     7921 tgcgtggtgg cgcaccacgc ggccagtggt gaacgccggc gccgggcgcg ctcaatcggc
     7981 ggccaagggc ggccgcagtg ccgccggcgc ggccgcgtcc agcggttcct cgcagccggt
     8041 gccaccgtcg gtgcgcggcc cgctcaggtg gcgccaggca aagccggcgt agagcagcca
     8101 ggccacggcc caggccagcg ccgccagcca ccacgccgcc agcgtgccgc ccgccgccca
     8161 gggcgtgctc gccagcacgc gcgcacccac cgccgccacc agcagggcgc tggccaccac
     8221 gcgccagcgg cgatcgtcga gcggcaggcc ggcgtggatg cggccggcga tgttcatcac
     8281 cgccagcacg cccaggccca tgctgcccac ggtcagggca tggcgcccgg ccgaaggcgc
     8341 ccacggcgcc cccagccagg ccaggcccag cagggcatag ccggccgcca tgcacaggta
     8401 gaccgcgtag agcggccaca cccagcggtg cccgagggcg cggccgaaat gccattcact
     8461 catcagcgcc agcaccgccg ccgacgccgc cagcgccagc cagcccagcg tgggctcggc
     8521 cacgcccagc agctccgtcc ccgtcagcag cgcgatgcag aagatcgcca gccggcgccg
     8581 gggtggaggt gcgcggtagt cggcctggtg cacgcccagg tggtccagca tgccgcgcag
     8641 caggcgcatg gatatgcggc tttgcgccag cacgatcagc accatcatca ggccgacgca
     8701 ggcgttgacc cagggcatgg cgtcgcgccc gccgagcgcg gcccaccaga agcccgcctc
     8761 cacgcccagc agggccagca gcgccagcgc aaagccctgg tgcgggcgca ggggctggcg
     8821 cagcaggcgc ggcaggctgc gccaggccag gctggcgacc agcgccaggt tcagcgctgc
     8881 cgtcaggggc acccacaggg cgggcagcag ggccaggccg accagcccga acaggcgcgc
     8941 cgccagccag agaccgaaca gcagcgcggt gtcgcgccgc ttgaattccg gcgtggcggt
     9001 gaattccggc accgccgtca gcagaaaccc cgtcaccgcg gccatgccga aaccccacac
     9061 catttcatgg gcgtgccagg cgctgccgct gagcggcccc ggcaccagcg gcagggtcgt
     9121 ggtcagcatc agcatccaca agcccatggc cagcgccgcc cacagcgccg ctccgagaaa
     9181 gaagggccga aagccgcagc acagcagtgg cgcagcccac agacgcgcaa gcaaggggcg
     9241 cggggccgcg ccgcggggtt cagccacagc catggcgacg cacctgctgg gggatgtcgg
     9301 ggtgggggct ggcccgcagc gcctcgtggg ccggcagcgc ggccggcgca tccaccggcg
     9361 gcggcaggtt gaaggcttcg cgcaccagtc ggtgcgccag caggcgcgcg gtctgccggt
     9421 agacccaggc gtcgtcgcgc gcggccagcg gcctatccaa gtgaaagcgc gccagcacgc
     9481 ggccggggtc ggggcgcagc agcaccagtt gctgtgccag gcgcacggcg tccatcagat
     9541 cgtgcgtgac cagcagggcg gcgcagccgc gctcgccgac ctgccgcgcc agcagggcgt
     9601 gcatctcggc gcgcaggccg acgtcgagcg ccgaaaacgg ctcgtccagc agcagcagcg
     9661 ccggatcgac cgcgaacgcg cgtgccagcg cggcgcggct ctgcatgccg ccggacagct
     9721 cggccgggaa cttggcgtgg tcttccgggc gcaggccgac ctgcgctgcc agcgcggcgg
     9781 cgcgcgcgtg gcattcgctg cgcggcaagc cgcgcgcctt caggcccagc gcgatgttgt
     9841 cgagcagacg cttccagggc agcaggctgg gttgctggaa cacgatggcg ctgcgcgaga
     9901 agtcggtctg catctgaccc tcggtgggcg tcagcaggcc ggcggcgatg cgcagcagtg
     9961 tggtcttgcc gcagcccgag cggcccagca gggccagcac ctcgcccggg gcgatttgca
    10021 ggtcgatccc ctccagcacc gtctcggggc cgaagcggtg gcccacgccg cgcagcgcca
    10081 gcgccgtcat gccgcgcctc cgccgtcagc gcgccagcgc tccatctcgc gtcggatcgg
    10141 ctccagcagc aggtactcgg ccgccagcag caggccgacc acggcgctca cccaggccag
    10201 cgtgccgccc atgtcgaggt ggctgcgggt ggccgccagt tgcgcgccgg cgccgtcgga
    10261 ggtggccagc agctcggcca tcagcaccac cttccacgac acccccagcg ccgtgatgca
    10321 ggcggcgaac aggtaggagg cgatgtgcgg caggtagacg tcggcaaagc gctgcgtcag
    10381 cggcaggcga taggcttgcg ccagctcgcg cagacggccg tcgagcgtgc gcgtgccctg
    10441 cagggcgccg acgaagatga tcgggaagca ggccacgaag acggtgaaca ccggcgtcgc
    10501 gtcgccgctg ccgaaccaca gcagggccag caccagccag gcgatcggcg gcatgccgat
    10561 cagcaacgtg accagcggcc gcgccatcat ggtggcggtg atcgaggcgc cggccgccat
    10621 gcccagcacg gcgccggcca gcaccgacag gccgtagccg tagagggcgc gccgcgccgt
    10681 ggctcccagg tccagccagg cggcgggcga tcgcaggatc tgggtcaggc tggccagggt
    10741 ctcctgcggc gtcggcagca ccagcggccc cagctggcgc gccagcgcgt cccaggcggc
    10801 caccagcagc agcacgctgg cggcgccggt ccagccgctc cacagatagt ggccgacgcg
    10861 cgccggccag cgggcgcggc gggtgtcggt caggtcggcg gcggtgcggc ggtcggccat
    10921 cgatgcgctt caggcgccgg caaggaagcc ggcgtccggc agtttgccac ccagcagctg
    10981 tgggtggcgc ttgaacaggc gctcgaacag gaacagcagc tcgtcgcgcg cctgcgccgc
    11041 cggcacggcg tccagcggcg cggcgcccag gctgtcggcc acggcttcgg ccgacaggcg
    11101 cgactcgacc gccgccacct cagcgccgca ggcggcgcgc tggctctggc accaggcggt
    11161 ggcgccggcg taggcgtcac ggaagcgggc aatcagcgcc gcgtcctgcg ctgccgcccc
    11221 cagcgccacg atgccggcct gggcgatgcg cggccggcgc ctgaaggcgc gtgcccattc
    11281 ctgctggatg tcgacggcgc gatgcaactc gggggcgatg gcgctgaccg ggaaggactg
    11341 cgtcttgcgc agcgccatcg acaccgccgg ctcggccagc agggcgtggc gcacgcgccg
    11401 cgtgatgagc agctgcatgg cgtccagcgg cgaggcgacg tagcgcaggc ggaagtcctt
    11461 tttcgggtcc agccccaggg cctgcgccgc ttcctgaaac accagatcgg gcatgtcctc
    11521 gcgaaacggc atggcgattt cctcgccgcg gaagtcggcc agcgtccggc gttcggcatc
    11581 gcgcgagacc aggaacagga tgccccacac cgacacgttg accagcttca gcggcacgcc
    11641 ccggttgtac aggttggcgg cgacgttggt cggcatggcg acgaagtcgg cctcgccacg
    11701 cagcgccagc gcgcgcagct ggtcggggtc gcgccagggc aggtagcgca cgcgttcggc
    11761 caggccgggc acgcggccct gggcggccag gtggatcagc gggttgctga cgctggccgg
    11821 cgggccggac aggcgcagct cgggcagcgt ggcggcacgc gcccccagca gcgcgccggg
    11881 cgcggcaccc agcagcgcgg tgcattgcag gaaacgacgt ctacaggtca tggcgattag
    11941 aactggagct ggtagctgac gaccagcgag cggcccggcg ccggcacttc gcgtcctgcc
    12001 aggccctcgg ccaggtgctc atggtatttg cggtcggtca ggttggtgac ggccaggcgc
    12061 agctggtgcg ggccgcgcgc ccaggtggcg ccgatatcga gcgtggtgaa gccgggcgtg
    12121 gcgtcttcca gcccacgtgc gaacacggtg gcgacgcggt cctggcgcca ggcgtggcgc
    12181 actcgcgcgt cggcgctcca gccggcgccc aagcggcctt cccagctcag gttaagctcg
    12241 tcggcgggca tttggtagag cggttcgttg aagtcgttgt tgcggccgcg cagacgggaa
    12301 tagcccaggc ccagccagtg gccacgcgca aactgccagc gggcgcccag ctccaggccg
    12361 gagatgcgca cgctgcccag gttgatggtc tggcgcaccg gggtgccgtt ctgggtctgg
    12421 ccggtggcgc gaccggtgat gtagtcgctg atgcggttgc ggtagacggc ggcgaaccac
    12481 tccagctgct cggacttgcc cttgacgccg atctcgatct gctgggcgat ctcgggccgg
    12541 atctgcgggt tgcccaggta gaagtagccg tcgccgcgcg gcgaagcctc gaagcgctcg
    12601 cgcatctcgc ccggccggaa gccgcgcgac aggctggcgt aggggcgcag cagcgggtcg
    12661 agttcgtaga tcaggcccag gttgcccgac acggcgccgt cgccgcgctt cagaccggtg
    12721 gtgacgctgc cgttggccac cgaggcggcg ttgccctgca cccggtcgta acgcagaccg
    12781 gccaccacgc ccagcgcgcc gaagcgcatg tcgtcctgca cgtacaggcc ggcggcctgc
    12841 acccagccgt cctcgaacgg gttgttgcgc acgtagctgc cgttgttgaa gatgcggcgg
    12901 tcgggcgagc ccgtcatgcg ccagccgttg aagccgacgc tgatcagatg gtgttcgttc
    12961 gccgcccagt cggcgcggac gtccaggccg gtggtgtcga aggcgacgtc gttggtggtg
    13021 gtgtcacgct gcagcgggcc gttggcccag gcgttgatgg tgcgatcgac gctctggcgg
    13081 tacagccgtg catcgaagtt gaccggcccg cgccccttgt ggctgtagcc gatctccatc
    13141 agctggcgcg actggcgcgg cgagtgaatg gtggtggagc ccaccatggg gttggcgttg
    13201 ggctgggtcg aaccggggta ccagacgtcg ttcaggttct cgcgctgcag cgacaggcgg
    13261 atctgttggg cgccgtcgat gcggtagcgg tactggccga tcagcgcgtg ctgctcgtag
    13321 ccggtgcgct cgacgcgctg cccgccggcg cggtagttgc cgtggtcggc accggcagct
    13381 cccagcatca gggcgtggtc accgccgctc cagttcacca cgccgctggc accgacgctg
    13441 cggtcgccac tggccgcgcg caggcccagg cggccggaga cgccggcgtc gaaacgcgcc
    13501 tggggcagca acacgttgat ggcgccgccc agcgcgcccg agccatacag caccgaggcc
    13561 ggcccgcgca gcacttccac ccgctcggcc aggccgagcg agacgaagct gccgatggcg
    13621 ccatagggct gggccgagtt caggcgcatg ccgtcgacca tcagcgccag cgattccttc
    13681 ttgagcccgc gcaccactgg gttcaggctg gcgctgccgt cgctggtggc ggccaggccg
    13741 ggctcgccct gcagggcctg gcccaggctg gtggcgccct tgcgccggat ctcctgggcg
    13801 tcgatgacgt cgatcgacgc cggggtgtcc agcgtatctg ccgcgtagcc cttggccgtg
    13861 acggcgattt cgggcagctg gttgtcggct ggcgctggcg ccgcggtctg ggcctgcgcg
    13921 ctggcgcaca ggccaagcag cgccagggca agcggggtcg ggcgaagatt cggcagtggt
    13981 ccgcggggcc ggcggaaggt gagcttggct cggctggcgt cggtgggcgt cgggcaggcg
    14041 gtgggcagct tcatgggagg gtccgtaacg gcgatggagg aagggcaatc gctggcggac
    14101 agaccgggct gctggctggc tgatgaaggt caccgatgcc ccggcggcgc gcgctgagcg
    14161 ctcgcatgcc gccagcgggc gcacgcccgc gcgcccatgc cgtggcaggg gtggggcgat
    14221 cgggggctgt cgggtgcatc ggttcgaatg cgaatgatta tcattgaatc attcaccatt
    14281 cgacaccgtt caaccttgat ctgaaacaag ttcacccgag agtggggcgt gaaagggtgg
    14341 ccgtggcgcc aggggccgcg gcggggccgg gcgcctccgt gccgggcggc atggcccacg
    14401 tcacgcagcg gacaagcgca gcgcaggggg cgccccataa tcggcgcgat gtccaccctg
    14461 cgcaacaccc tcgactttct gctctacgac tggctggcgg ccgaccggct gaccgagcgc
    14521 ccacgatttg ccgaccactc gcgcgagact ttcgacgccg tgctcgacac ctgcgagcgc
    14581 atcgcgcgcg aaaagtacgc ccctttcaat cgcctggtcg acaccgaaga accacgcacc
    14641 gaaaccgcac ccgatggcag cctgcgcgtg gtgctgccac gcgccaccca cgaggcgcgc
    14701 accgcctacg ccgaatccgg catgctggcg gcggcgcagg atcatgagca cggcggcatg
    14761 cagctgccct acctggtcga ggcggcggcc aacgccttct tcggcaaggc ctcgatcagc
    14821 atcggcggca acctgctcac cgtgggcaac gccaacctcc tgatggcgca cggcacgccg
    14881 ctgcaaaaac aggtgtttgc cgccaacgaa ttcaatggcc gctgggccgg caccatgtgc
    14941 ctgagcgagc cgcaggccgg ctccagcctg agcgacatcg ccacgcgcgc cgagctggaa
    15001 gacgccagcg acccgctggg cccgcgctac cgcctgcgtg gcaacaagat gtggatctcg
    15061 gccggcgagc acgagatgac tgaaaacatc gtgcacctgg tgctggccaa gatccccggc
    15121 gccgacggca agctggtgcc gggcgtcaag ggcatttcgc tgttcatcgt gcccaagcgg
    15181 ctggtggaca ccgaaggccg cctgaccggc gtgcgcaacg atgtcgcctt ggccggcctg
    15241 aaccacaaac tgggctggcg cggcaccacc aacacgctgc tcaactttgg cgagggcaag
    15301 ttcccggtcg acggccagcc cggcgccgtg ggctacctgg tgggccagcc gggcgagggc
    15361 ctgcgctgta tgttccacat gatgaacgag gcgcgcatcg gcatcggcat ggccgccacc
    15421 atgctggggt tggccggcta cgaggcttcg ctcgactacg ccaagacgcg catccagggc
    15481 cggcccatcg gtggtagcgg caaagatgcc gcccagccgc cggtgcgcct gatcgagcac
    15541 gccgacatcc gccgcatgct gctggcgcag aaaagctggt gcgaaggcgc gctggcgctg
    15601 ctgctgtact gcgcccgtct ggtcgacgag cagcacaccg gcgcgcccga agcggccgac
    15661 gaggcgcgcc tgctgctgga ggtgctgacc cccgtggcca agagctggcc gagcgaaaac
    15721 tgcctggagg ccaacagcct ggccatccag atccacggcg gctacggcta cacgcgcgac
    15781 ttcccggtcg agcagtactg gcgcgacaac cgcctgaaca tgatccacga aggcacgcac
    15841 ggcatccagg ccatggacct gctggggcgc aaggtgcgca tggagcaggg ccgggggctg
    15901 gcgctgctgg ccgcgcgcgt gcaggccacc attgcgcggg cgcagacgca cgcggcgctg
    15961 cagcccgacg ccgccgcgct gggcgccgcg ctcgatcgca ttcagcaggc cacccgcgcc
    16021 gcctgggcag acgccgacgc caacgccgcg ctggccaacg ccgtgcccta catgcaggcc
    16081 ttcggccatg tggtcatcgc ctggatctgg ctggagctgt cgctcgcttc aagtactaca
    16141 aaaacaatag ctgctggcgc aggtgtggcg ggcgccagaa cctattttta tcggtatgaa
    16201 ttgcccaaaa tcgacgcctg gctgcgcgtg gtggaaagcc gcgacctgac ctgcgcccaa
    16261 ctgcccgagg aggccttctg atgctcaagc acatcgtcat gtggaaattc aaggaccagg
    16321 ccgaaggcgc cgacaaggcc gccaacctgg ccaaggccgt cgctctgctg gagggttgtg
    16381 ccgatctggt gcccggcacg ctgcggctcg aggtggcgac cgcccagccg gggctggaag
    16441 ccacctacga cctggtgctc tacagcgagt ttgaaggccg cgcggcgctg gatgcctatc
    16501 agaaccaccc cacccacgtg gccgtcaaac cctttctgcg cgccgtggtg agcgagcgcc
    16561 agtgcatgga ctacgagctc tgaacccgcg accaggatcc gacatgaccc gcaccgtaca
    16621 gcaactgttc gaccttaagg gcaagaccgc cctcatcacc ggcggctcgc gcggcctggg
    16681 cttgcagatg gcgcacgccc tgggcgaggc cggcgcgcgc ctgatgctca gctcgcgcaa
    16741 ggcgggcgac ctggagcagg ccgtggccga gctgcaggcc gccggcatcg acgcgcgctg
    16801 gatcgccgcc gactgcagca aggaagaaga cacgcgccgc ctggccgacg aaacgctcga
    16861 acgcatgggc gccatcgaca tcctggtcaa caacgccggc gccagctggg gcgcggcggc
    16921 cgaggagcac ccggtggccg cctgggacaa ggtgatgaac ctgaacgtgc gcggctactt
    16981 catcctcagc cagcaggtcg ccaacggctg gatgattccc aacaaggccg ggcgcatcat
    17041 caacatcgcc tccatcgccg gcctgaacgg caacccgccc gagatgaaga ccctcgccta
    17101 caacaccagc aagaccgccg tcatcggctt cacgcgcacg ctggcggccg agtgggggcg
    17161 ctttggcatc accgtcaacg ccatctgccc gggttttttc cgcaccaaaa tggccaatgg
    17221 cctgatcgac cagctgggcg agggcaagat ggccgccgcc gcgccgctgg gccgcctggg
    17281 cgatgaggaa gacctgaagg gcatcaccct gctgtacgcc agcgacgccg gcaagcacat
    17341 caccggccag tggctggcgg tggacggcgg cgtcagcgtg gtgctggggg cggcatgaat
    17401 ctggcaccca cgctcctccc ccgctgcgcg tggtgcgctg ccccccgagg gggctccggc
    17461 cgccttggga gcggctcggc ggcggccggg ctgcctttca caagcgtcaa ctgaactttg
    17521 accatgagcc tggacttcgg cgcccacatt cccttcgtcc atcacctggg cttcacgctg
    17581 gagcgcttcg aaggcggcgc atcaacgctg cacttcgcgc cccgggccga gcactgcaac
    17641 tccttcggcg tcgcccacgg cggcgcgctg atgacctttc tggacgtcgt catggccacc
    17701 gccgcgcgca gcgccaaccc cggcagcggc atcgtcacca tcgagatgaa aaccagcttc
    17761 atgcgaccgg ctccggcggc cggcggcgtg ctggtggggc agggccggct ggcgcaccgc
    17821 acgcgcagca tggcgttcgt cgaaggctcg gtgttcgatg ccgaaggtca gctgtgcgcc
    17881 cagtccaccg gcaccttcaa gtacgtgcca cgcgcccagc cgacgggcca gcccgccggg
    17941 ccattgccca ccgactgacg ctgctttcag gttttttcgg ccgccagcgc ccaccagaca
    18001 agcgccagca gctattcttt ccatagtcca ccgatcgagg acccgcatgc ccaccaaccg
    18061 ccagatcctg ctcgacaacc gcccgcaggg cgaagccacg gccagcaact ttcgcctggt
    18121 gtccagcgaa accccagcgc tgcgcgacgg ccaggtgctg gtgcgccacc attacctgag
    18181 cctggacccg tacatgcgcg ggcgcatgaa cgacggcaag agctacgccc agccccagcc
    18241 gctgggcgag gtgatgatcg gcggcacggt gggcgaggtg ctgcagtcgc gccacccgcg
    18301 ctttcagccg ggcgatgccg tggtgggcat gggcggctgg caggaataca gcgtggtcga
    18361 cgccgaccag cccggcctgc tgcgcaaggt cgacaccacc catgtgccgc tgtcgtacta
    18421 cctgggggcg gtcggcatgc cgggcgtcac cgcctggtac gggctgacgc agatcatcgc
    18481 cccgcaggcg ggcgaaaccg tggtgatcag cgccgccagc ggcgccgtcg gcagcgcttt
    18541 cgcggccgtg gccaaggcgc gcggctgccg ggtggtgggc atcgccggcg gcccggaaaa
    18601 gtgccgctac gcgcaggagg agctgcactt cgacgcctgc atcgactacc gccagcaccc
    18661 cgatatcaag tccatggcgc aggcgctgaa ggccgcctgc ccgcagggcg tcgacggcta
    18721 tttcgagaac gtcggtggct acttgctcga cgccgtgctg ctgcgcacca acgccttcgc
    18781 gcgcatcgcc gtgtgcggca tgatcgccgg ctacgacggc cagccgctgc cgctgcagaa
    18841 cccggctttg atcctcatca accggctcaa ggtgcagggc tttatcgtca gcgagcacat
    18901 ggacctctgg ccgcaggcgc tgcaggagct gggttcgctg gtggcgcagg gcaggttgcg
    18961 cccgcgcgaa tcgatcgccc tgggcatcga aagcgcgcca gaggcctttc tgggcttgct
    19021 caagggccgc aacttcggca agcaactggt gaaactgatc tgatcgaccg tttgccttcg
    19081 gagacccgcc catgagcacc gtcacgcacg aggtactgaa ccagcccacg ccgctgcagg
    19141 acatcaacct gttcgagggc catcaggccc tgcgcgacgc gctgcgcctg catgcccccg
    19201 ggctcgacac cgccagcctg gcgtccctgg gcaagctggc cggcagcgcc gagatgcagc
    19261 gccacgcgcg cctggccaac gtgcacacgc ccgagctgcg cacgcacgac cgctttggcc
    19321 accgggtgga tgaggtcgag ttccacccca gctaccacgc cctgatgtcg gccgccaccg
    19381 ccgccg
//
LOCUS       DAOOOG010000030        19135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_2919_length_19135_cov_28.470231, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000030 DAOOOG010000000
VERSION     DAOOOG010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 19135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19135
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2919_length_19135_cov_28.470231"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="PK242_04070"
     CDS             <1..1265
                     /locus_tag="PK242_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOZ93226.1"
                     /translation="FAMSELAVASSRKARLTAMEESGDAGASAALRLMEQPTQFLSTV
                     QVGITSIGMLNGIVGEAAFSGPVADGLTRAGLSAGAASIAATALVVVAITFTTIVFGE
                     LVPKRIGQMFPETVARWIARPMGWLASAAKPFVALLSLCTQATLKLLRIDESHGRAVT
                     EEEISASLEEGVDAGLIEEHEHQMVRNVFHLDDRSLISLMVPRSEIDWLKAEASVAEA
                     LDFVAGQGERKAHSWYPVCDGGLDNVIGIISVARLLELGRHDQDLLRAHVIPPTFVPE
                     TLSALELLEQLRARSGRMMLVVDEYGVLQGLITQHDFLEAITGELNPGAHIEAWAIPL
                     GSGGWQLDGMMPISEMKARLDIDGDLPHEDRGRYNTVAGLLMAEAGRLPAVGDVIDCA
                     GWSFEVTGLDGRRIDKVLARPLEISEST"
     gene            1342..1635
                     /locus_tag="PK242_04075"
     CDS             1342..1635
                     /locus_tag="PK242_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HOZ93227.1"
                     /translation="MATYQELLKQQAALAVQVEEARKREIADAVARVRQIVNEYDLTE
                     KDVFPAGRRAATPRMAGKVAPKYRDPATGQTWTGRGKPPKWIDGKDRNLFRIG"
     gene            1847..2173
                     /gene="grxD"
                     /locus_tag="PK242_04080"
     CDS             1847..2173
                     /gene="grxD"
                     /locus_tag="PK242_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HOZ93228.1"
                     /translation="MDQTTQQHIEGLVKGNDIVLFMKGDANFPMCGFSGRAVQILKAC
                     GVDTSAVRTVNVLDDEAVRQGIKEYSQWPTIPQLYVRGEFIGGSDIMMEMYESGELQQ
                     LVGGKS"
     gene            complement(2243..3280)
                     /locus_tag="PK242_04085"
     CDS             complement(2243..3280)
                     /locus_tag="PK242_04085"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HOZ93229.1"
                     /translation="MFDVYIREAGARFGLGEQALTVAQMLLADMTSPDTGGLMGFLER
                     FKSAGLGPVIQSWLGGGPSAQPISNSQLETVLGASGGLLSRLTGLLDAPRDNVTTAIG
                     YLLPAFVGRLTPGGSLPSSLPPEVLSLAAAGRDLLTAPAAASAPVAAGQPGMLRWLPW
                     VIVAAAALIGLAYCGKQEQATPSAPATPAVDAASTAAPAAGASAAADAASPAAPSASP
                     AAAAASGPEPGAAVEALTDAASRPALQVFFDSGKAEVHPDLAARAADLLAYVKANQGV
                     QVVIAGFNDPTGDAAANAELSRQRAQAVQAALVAAGLAAERTVLDKPADTTATTSSLA
                     AARRVDVTLRP"
     gene            3514..4815
                     /locus_tag="PK242_04090"
     CDS             3514..4815
                     /locus_tag="PK242_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOZ93230.1"
                     /translation="MSLTQSLLLIALLIAVSAFFSLAEIALAASRRLRLRQLADDGDA
                     RALRVLRVQEQPGFYFTTVQIGLNAVAILGGIVGEGLLGPYLAELLRLWLPERTAANL
                     SLVGSVAAVTSLFVVFADLVPKRLGMAEPERVAMAMVGPMGHVLLLFKPIVWLYTACT
                     DLVMRLLGLPMQRDERITPDDILALAEAGTQAGVLDRPGQQVIENVFELDTRTVTSAM
                     TPRDRIAWLRHDADDAEIRLRIAAEPFSTYPVCEGELDRVLGYVDAKDLFQRVLHQRP
                     IELTEPALLHKVVVVPDSLSLSEVLHQFRQQHEDFALIVNEYSTVVGVVTLNDVMSTV
                     MGELVTPEDEEPIVLRDDGSWLIDGHTPMADVQRALNLDEPPSAEAYDTLAGFLMVML
                     RRVPRRTDSVQWGGHRFEVMDVDNYRIDQVLVTRSERSAPE"
     gene            complement(4855..5433)
                     /locus_tag="PK242_04095"
     CDS             complement(4855..5433)
                     /locus_tag="PK242_04095"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOZ93231.1"
                     /translation="MPGALIFQERALAGGQLEEPLADAVRTALSSAIANDAPPLPVFA
                     STDARLRYLRWLSGMSDRLYSRKKDFNTRIEFLQTVWYESQRAGLETSLVLGLIQVES
                     AFRKYAVSSVGARGYMQVMPFWSRVIGDGDAGKLFHMQTNLRFGCVILRHYLERERGD
                     LFMALGRYNGSRGRPQYPNAVFGAQKRWVLSA"
     gene            complement(5505..7250)
                     /locus_tag="PK242_04100"
     CDS             complement(5505..7250)
                     /locus_tag="PK242_04100"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOZ93232.1"
                     /translation="MKASQFYISTLKEAPADAEIVSHQLMTRAGMIKKLGAGIYTYMP
                     MGLRVIRKVEAIVREEMNRAGAVECVMPVVQPGELWSESGRFQTMGPELMRIRDRHER
                     DFVVQPTSEEAVTDIARQDLRSYKQLPRNLYQIQTKFRDERRPRFGLMRGREFIMKDA
                     YSFDRDEPGAKRSYQAMARAYRAIFDRFGLRYRAVAADSGAIGGDLSEEFQVIAATGE
                     DAIVYCPGSDYAANMEKAEALAPAGPRPAPAQALTNTPTPGKATCAEVAELLGLPLAR
                     TVKSLVLATDELDAQGAVAKTQIWLLLLRGDHDMNEIKAAKVPGLNAGFRFATVPEIQ
                     AHFGTPPGYLGPIGLKRPVKVVADREVALMADWVCGANAEGHHLTGVNWGRDLPEPDV
                     VADLRNVVAGDASPDGRGVLAIERGIEIGHIFYLGTKYSRAMNATFLDEDGKPKPFEM
                     GCYGIGITRLPAAAIEQNHDARGIIWPDAIAPFTVVICPVGMDRSESVKTAAEALYAE
                     LLAAGVDVILDDRGERPGVMFADWELIGVPHRVTIGDKGLKDGQVEYQHRRDSTATRV
                     PVDGIAAFVTGRLAV"
     gene            7358..8008
                     /locus_tag="PK242_04105"
     CDS             7358..8008
                     /locus_tag="PK242_04105"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HOZ93233.1"
                     /translation="MLDREGFRPNVGIILLNQKNQVFWGKRIRTHSWQFPQGGIDRGE
                     SPEQAMYRELHEEVGLQPEHVRIVARTRDWLRYEVPDRFIRRDARGHYKGQKQIWYLL
                     QLIAQDWQLNLRATSHPEFDAWRWNDYWVPLDVVVEFKRGVYEMALTELARYLPRTEN
                     RNRYLRGGVRARDGGELPAIQTRDRLARAASAAIALPPGATLEGVPRPPFGPPDKP"
     gene            8005..8547
                     /locus_tag="PK242_04110"
     CDS             8005..8547
                     /locus_tag="PK242_04110"
                     /inference="COORDINATES: protein motif:HMM:NF020331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HOZ93234.1"
                     /translation="MSRRLRPPGRRAWLACLLAACAGLFSAPGAAQYADLDRADWQED
                     PVPPPPAYSLERLLEIDMPKTASVRMGIDPQTLSVNHQSGVVRYVVVARGTSAVNASY
                     EGIRCTTGEFRVYARQVQGGEWTPSTDSGWKSMRGQSSVLVQHPLRLARDGLCLGPSA
                     RQTVSEMVRELKTGNRSLYY"
     gene            complement(8599..9744)
                     /gene="proB"
                     /locus_tag="PK242_04115"
     CDS             complement(8599..9744)
                     /gene="proB"
                     /locus_tag="PK242_04115"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOZ93235.1"
                     /translation="MTQNSVSEVLRQAQRIVIKVGSSLVTNEGRGLDETAIGEWCRQM
                     ARLAGQADLPREVVMVSSGAIAEGMKRLGWTQRPSEVNELQAAAAVGQMGLAQMYETK
                     LREQGLRSAQVLLTHADLADRERYLNARSTLLTLLEHRVLPVINENDTVVTDEIKVGD
                     NDTLGALVANLIEADLLVILTDQKGLYTADPRANPQAEFVHEARAGDPALERMAGGAG
                     SSIGKGGMLTKILAAKRAARSGASTVIAWGREPDVLLRLSRGERIGTLLVAPTQKLQA
                     RKQWMADHLQLRGALVIDDGAVAKLRGAGKSLLPIGMTQVEGAFARGDVVAIRDARGA
                     EVARGLANYSSAEARLLCRRPSGDITVLLGYMAEPEMVHRDNMVLIR"
     gene            complement(9784..10863)
                     /gene="obgE"
                     /locus_tag="PK242_04120"
     CDS             complement(9784..10863)
                     /gene="obgE"
                     /locus_tag="PK242_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOZ93236.1"
                     /translation="MKFVDEAFIDVAAGDGGNGCSSFRREKFREFGGPDGGNGGRGGH
                     VFALADPNLNTLVDFRYARRHEARRGQHGMGSDMFGAAGDDITLRMPVGTIISDADTG
                     EVLHELLQPGQAVLVAKGGDGGFGNLHFKSSTNRAPRQKTPGWPGEKRSLKLELKVLA
                     DVGLLGMPNAGKSTFIAAVSNARPKIADYPFTTLYPNLGVVRVGPEQSFVVADIPGLI
                     EGASEGAGLGHQFLRHLQRTRLLLHIVDIAPFDEGVDPVVQARAIVAELKKYDPSLHA
                     KPRWLVLNKLDMVPPAEREARVREFVRRLRWKGPVFQISALTREGCEPLIQAVYQFVR
                     AQQVADAPAEPPDPRFADAAEPHQK"
     gene            complement(10954..11214)
                     /gene="rpmA"
                     /locus_tag="PK242_04125"
     CDS             complement(10954..11214)
                     /gene="rpmA"
                     /locus_tag="PK242_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOZ93237.1"
                     /translation="MAQKKGGGSTRNGRDSKPKMLGVKVFGGQTVSAGSIIVRQRGTK
                     FHAGSNVGVGKDHTLFALVDGQVSFATKGALNKHVVQVTPAA"
     gene            complement(11221..11550)
                     /gene="rplU"
                     /locus_tag="PK242_04130"
     CDS             complement(11221..11550)
                     /gene="rplU"
                     /locus_tag="PK242_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOZ93238.1"
                     /translation="MYAVIKTGGKQYRVAAGEKIKVEQIAADVGQEITLDQVLAVGNG
                     SDLTVGAPLVAGASVTAKVVSHGKHDKVRIFKMRRRKHYQKRQGHRQGYTELEIGAIN
                     GAGASKE"
     gene            11751..12734
                     /locus_tag="PK242_04135"
     CDS             11751..12734
                     /locus_tag="PK242_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOZ93239.1"
                     /translation="MTTSQNPTARALALAADDMRQVDAIISRRLDSDVPLVGQVAGYI
                     VSSGGKRLRPVLLLLMCQALGCLHPQRHTLAAVVEFIHTATLLHDDVVDESTLRRGRA
                     TANQTFGNPASVLVGDFLYSRAFQMMVESHSMRVMQVLADATNVIAEGEVLQLMNMHD
                     ASLDEAAYLRVIRSKTAKLFEASCRLAAILANSPPALEQACAEYGQSLGTAFQVIDDV
                     LDYDGDSGEMGKNLGDDLREGKATLPLILAMQLGNADEARIVRQAIETGNTQQLPRII
                     DIVRCTGALDATRAAAAAQAERAMQAARALPANEATNSLLELAAQLLQRRS"
     gene            12757..12833
                     /locus_tag="PK242_04140"
     tRNA            12757..12833
                     /locus_tag="PK242_04140"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12791..12793,aa:Pro,seq:cgg)
     gene            12994..14733
                     /gene="pilB"
                     /locus_tag="PK242_04145"
     CDS             12994..14733
                     /gene="pilB"
                     /locus_tag="PK242_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HOZ93240.1"
                     /translation="MAIADPVSKTSLPAALPGVGRALVMAGKITQQTADDAFRKAKAA
                     NRPFIAELIQSGAISAAELAHTMSQAFSAPLLDVDALDIEHLPKGLLDPKLSAHHRIV
                     ALNKRGSRLIIATADPSDHEAAEKVKFATQMGIEWVIGEHDKLSRLIEVLGKSLSDAM
                     DSIIGGDFEFDEASLASDAQTEDSDTTQTEVEDAPVVKFLHKMMVDAVNMRASDLHFE
                     PYEHQYRVRFRIDGELREIASPPTAIKEKLASRIKVLSRLDISEKRVPQDGRMKLKMG
                     ADRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYEPEEKERLLKAIERPYGMI
                     LVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAVA
                     LRAFLRQDPDIIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPATLTRMRNMGIP
                     PFNIASSVILITAQRLARRLCTQCKAPVDIPRETLREAGFADNELDGSWTPYGPVGCA
                     ACHNGYKGRVGIYEVMPISEEIQRIILADGSELEIAEQARREGVRSLRAAGLHKVKTG
                     VTSLEEVLGVTNQ"
     gene            14773..15996
                     /locus_tag="PK242_04150"
     CDS             14773..15996
                     /locus_tag="PK242_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOZ93241.1"
                     /translation="MATQVAGAKGVKEFVFEWEGKDRNGRVVRGETRAGGENQVQATL
                     RRQGIFVTKIKKRRMRAGKRIKPKDIAIFTRQLATMMKAGVPLLQSFDIVARGNPNPS
                     VTKLLNDIRADVETGTSLSAAFRKYPLYFNSLYCNLVEAGEAAGILEDLLDRLATYME
                     KTEAIKSKIKSALMYPIAVIVVAFVVVAIIMIFVIPAFKEVFSSFGADLPAPTLFVIA
                     MSEFFVQWWWLIFGGLGFGVYFFMQAWKRNEKMQMFMDRLLLKIPIFGELIDKAVVAR
                     WTRTLSTMFAAGVPLVESLDSVGGAAGNSLYKDATDKIQTEVATGTSLTAAMTNANVF
                     PSMVLQMTAIGEESGALDHMLGKAADFYEAEVDEMVAGLSSLMEPIIIVVLGTVIGGI
                     VVSMYLPIFKLGQVV"
     gene            15996..16838
                     /locus_tag="PK242_04155"
     CDS             15996..16838
                     /locus_tag="PK242_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOZ93242.1"
                     /translation="MPDGLVVMAAVGGLFGLLVGSFLNVVIHRLPQMMERQWAAECAE
                     LNGQPAAPQAPFNLLVPRSRCGHCGHPIRWYENIPVLSYLALRGKCSACGTPIGLRYP
                     LVELATGLLFGWCVWRWGWSWTAAAWCLFAAMLVALTLIDWDTTLLPDDLTLPLLWLG
                     LIAATLGLTGVRLSDAVWGAVAGYLSLWLVYWGFKLATGKEGMGFGDFKLFAALGAWF
                     GWQALVPIILMASVIGALVGIGMKLLSALREGGVVPFGPFLALGGITAMVLGPQTILR
                     AVGL"
     gene            16841..17449
                     /gene="coaE"
                     /locus_tag="PK242_04160"
     CDS             16841..17449
                     /gene="coaE"
                     /locus_tag="PK242_04160"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868017.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOZ93243.1"
                     /translation="MALRIGLTGGIGSGKSTVAAMLVEQGAALIDADAISRACTAAGG
                     AALPAIAREFGPQLIAADGALDRAAMRELVFRDPGARQRLEAIVHPLVGAETERQARQ
                     ALAQGRRLLVFDVPLLVESIERWRQRVDRIVVVDCEPETQIARVMARNQLPRAQVEQI
                     LAAQASRAQRLAVADAVIFNGADVTLQALRAQVRQLVDAFGI"
     gene            17478..18233
                     /gene="zapD"
                     /locus_tag="PK242_04165"
     CDS             17478..18233
                     /gene="zapD"
                     /locus_tag="PK242_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HOZ93244.1"
                     /translation="MILYEYPFNERIRTYLRLEHLFGRLAQLMPRDAPLDHHFALITL
                     FEALDVCARSDLKSDVLKDLDKQKTHLASFRGNPAVSEASLETMLASLEEAFEQLNQQ
                     HGKPGHELTDNEWLMAIRSRAVIPGGTCGFDLPAYHAWQHGQAAARRTELHRWAATLA
                     PMARAIALLLQILRDSGAPQKVMASAGQFQQTLPQGRSFQLLRLRIEASTQLIPEISG
                     NRLMVSVRLMRQDADGKLQLAREDAAFELALCL"
     gene            18254..18493
                     /locus_tag="PK242_04170"
     CDS             18254..18493
                     /locus_tag="PK242_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HOZ93245.1"
                     /translation="MPPPDAAPPSLPPRSVRCPACGGVSRYAADNPYRPFCSARCRQH
                     DLGAWASESFRLEAQEADGTDQAAPAHPSAPRFPQ"
     gene            complement(18550..19017)
                     /locus_tag="PK242_04175"
     CDS             complement(18550..19017)
                     /locus_tag="PK242_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOZ93246.1"
                     /translation="MSPVPNPVLTADGHLRRAQAGARSVVDVAVGVLQRPDGRFLLTS
                     RPAGKVYAGYWEFPGGKLEPGESVEAALARELHEELGLDIDPADVRRWREQLVDYPHA
                     LVRLHFCRLYRWGGTLQMREGQAFAWERLPVRVAPVLPGALPVLDWLQVERTA"
BASE COUNT         3101 a         6606 c         6361 g         3067 t
ORIGIN      
        1 tgttcgccat gtcggaactg gccgtggcct ccagccgcaa ggcgcggctg acggccatgg
       61 aagaaagcgg cgacgccggc gccagcgcag ccctcaggct gatggaacag ccaacgcagt
      121 ttctgtccac cgtgcaggtg ggcatcacct cgatcggtat gctcaacggc atcgtcggcg
      181 aagccgcctt cagcggcccg gtggccgacg ggctgacccg cgccggcctg agcgcgggtg
      241 ccgcctccat cgccgccacg gcgctggtgg tggtggccat caccttcacc accatcgtgt
      301 tcggcgaact ggtgcccaag cgcatcgggc agatgtttcc ggaaaccgtg gcgcgctgga
      361 tcgcgcgccc catgggctgg ctggcaagcg ccgccaagcc cttcgtggcg ctgctgtcgc
      421 tgtgcacgca ggcgacgctg aaattgctgc gcatcgacga gtcgcacggg cgtgccgtca
      481 ccgaggaaga gatttcggcc agcctggaag agggcgtgga cgccggcctg atcgaagaac
      541 acgagcacca gatggtgcgc aacgtgtttc acctggatga ccgttcgctg atctcgctga
      601 tggtgccgcg cagcgaaatc gactggctca aggccgaggc cagcgtggcc gaggcgctcg
      661 attttgtcgc cggccagggc gaacgcaagg cccactcctg gtatccggtc tgcgatggcg
      721 gtctggacaa cgtgatcggc attatctcgg tggcccgcct gctggaattg gggcgccatg
      781 accaggattt gctgcgcgcc catgtgatac ccccaacttt cgtgccggaa accctcagcg
      841 cgctcgaatt gctggaacag ctgcgtgccc gctccggccg catgatgctg gtggtggacg
      901 aatatggcgt gctgcagggc ctgatcaccc agcatgattt cctggaagcc atcaccggcg
      961 aactcaaccc gggcgcccat atcgaggcct gggccatccc cctgggcagc ggcggctggc
     1021 aactggacgg catgatgccg atttcggaaa tgaaggcccg gctcgacatc gacggcgacc
     1081 tgccgcacga agaccgcggc cgctacaaca ccgtggccgg cctgctgatg gccgaagccg
     1141 gccggttgcc ggccgtaggc gacgtcatcg actgcgccgg ctggtctttc gaggtgaccg
     1201 ggctggatgg gcgtcgcatc gacaaagtgc tggcccgccc gctggaaata tccgaatcca
     1261 cctgagccat tcgattattg attcggggtt gccgatcgga tatattccga ccgcccatat
     1321 acatcagaaa gcgagaatat catggcaacc tatcaggaac tcctgaaaca gcaggcggcg
     1381 ctggccgtcc aggtggaaga agcacgcaag cgtgaaattg ccgacgccgt ggcccgggta
     1441 cgtcagatcg tgaatgaata tgacctgacc gaaaaagatg ttttccccgc cggccgccgc
     1501 gcggcgacgc ctagaatggc cggcaaggtg gcccccaaat accgcgatcc ggccaccggt
     1561 cagacctgga ccggccgcgg caagccgccc aagtggattg acggcaaaga ccgcaacctg
     1621 ttccggatcg gctgaaaccg gttgcaccgc accaggcaaa ggcgcccatc agggcgcctt
     1681 tttcatgacc ccgaccgacc gccgcgggcc ggcgcatcag cgacacgcag atgcgccccg
     1741 tgccaacgct gccgctgcgc gccgcgcctg gcacgatccc cctgctgcgg ctgtggcagg
     1801 tcgaaggtgg aataatcgga accatcacat tgaaggaacc ccagtcatgg atcagaccac
     1861 gcagcagcac atcgaaggcc tcgtcaaggg caacgacatc gtgctgttca tgaaaggtga
     1921 tgcgaacttc ccgatgtgcg gcttttccgg ccgcgccgta cagatcctga aagcctgcgg
     1981 cgtcgacacc agcgccgtca ggaccgtcaa cgtgctggat gacgaggcgg tgcgtcaggg
     2041 catcaaggaa tacagccagt ggcccaccat cccgcagctg tacgtccgcg gtgaattcat
     2101 cggcggctcg gacatcatga tggaaatgta cgagtcgggc gaactgcagc agctggtcgg
     2161 cggcaagagc tgactcggca ccgccgcgcc ccgcaccacc acggacgccg cggcgtccgt
     2221 ttttgttgtg cccctgccgg cgtcacggac gcagcgtcac gtccacccgg cgcgcggccg
     2281 ccagactgga cgtggtggcc gtggtgtcgg ccggcttgtc cagcaccgtg cgctcggctg
     2341 ccagaccggc cgccaccagc gcggcctgca ccgcctgggc gcgctggcgc gacagctcgg
     2401 cgttggcggc ggcgtcgccg gtcgggtcgt tgaagccagc gatgacgacc tgcaccccct
     2461 ggttggcctt cacgtaggcc agcagatcgg cggcgcgcgc cgccagatca gggtgcacct
     2521 cggccttgcc ggaatcgaag aacacctgca gcgcagggcg tgacgccgcg tcggtcagag
     2581 cctccacagc ggcgcccggc tcgggcccgg aggccgcggc agcggccggg ctggcgctgg
     2641 gcgcagccgg tgacgccgcg tcggcggccg cggacgcccc ggcggcaggc gcggccgtgc
     2701 tcgccgcgtc gacggccggc gtcgccggtg cgctgggggt cgcctgctcc tgcttgccgc
     2761 agtacgccag accgatcaac gccgcggccg ccacaatcac ccagggcagc caccggagca
     2821 tgccgggctg gcccgccgcc accggtgcgc tggccgcggc aggcgcggtc agcagatcgc
     2881 gccctgccgc cgccagactg agcacctcgg gcggcagcga cgacggcagg ctgcccccag
     2941 gcgtcaggcg cccgacgaag gcaggcagca ggtagccgat cgcggtggtg acgttgtcgc
     3001 gcggggcgtc cagcagcccc gtcaggcgcg acagcagccc gccactggcg cccagcacgg
     3061 tttccagctg gctgttgctg atcggctgtg cgctcgggcc accgccgagc caggactgga
     3121 tgacgggccc caggccggcg gatttgaaac gctccagaaa acccatcaac ccgccggtgt
     3181 cggggctggt catgtcggcc agcagcatct gggccacggt cagggcctgc tcgcccaggc
     3241 caaaacgggc accggcttca cgaatgtaga cgtcgaacat ggagatcctt caatggtggc
     3301 ttgtgtggat gggccgcacg caagcgcgcc ggcgtttcgg gcgggcgtct ggcccgtggt
     3361 ggatcgcaaa aattatagga agccgccgcc cacggcctgt gaccgcctgc ttccgccggc
     3421 cggtggccgc tgtgtacact gccctgcggc gcgtcgtgcc cgccgggccg acaaaagcgt
     3481 gcccgccgcg ccattggcgg ccaccccgga ttcatgagcc tgacccaaag cctgctgctg
     3541 atcgcacttc tcattgccgt cagcgcgttt ttttcgctgg ccgagatcgc cctggccgcc
     3601 tcgcgtcgtc tgcgcctgcg tcagctggcc gacgacgggg atgcgcgtgc gctgcgcgtg
     3661 ctgcgggtgc aggagcagcc ggggttttac ttcaccacgg tgcagatcgg cctgaacgcc
     3721 gtcgccatcc tgggcggcat cgtgggtgaa ggtctgctgg gaccgtacct ggccgagctg
     3781 ctgcggctgt ggttgcccga acggacggcg gcgaacctgt cgctggtcgg ctcggtggcg
     3841 gcggtgacct cgctgttcgt ggtgtttgcc gacctggtgc ccaaacgcct gggcatggcc
     3901 gagcccgaac gcgtggccat ggccatggtg gggccgatgg ggcatgtgct gctgctgttc
     3961 aagcccatcg tctggctcta caccgcctgc accgacctgg tgatgcgcct gctgggtctg
     4021 cccatgcagc gcgacgagcg catcacgccg gatgacatcc tggcgctggc cgaagccggc
     4081 acgcaggccg gcgtgctgga ccggcccggg cagcaggtga tcgagaacgt gttcgagctg
     4141 gacacgcgca ccgtcaccag cgccatgacg ccgcgcgacc gcatcgcctg gctgcgccac
     4201 gacgccgacg acgccgaaat ccgcctgcgc atagcggccg agccgttttc cacctacccg
     4261 gtgtgcgagg gcgaactgga ccgggtgctg ggctacgtgg acgccaagga cctgtttcag
     4321 cgcgtgctcc accagcgccc gatcgagctg accgaaccgg cgctgctgca caaggtggtg
     4381 gtggtgcccg acagcctgag cctgtcggag gtgctgcacc agttccgcca gcagcacgag
     4441 gatttcgccc tgatcgtcaa cgaatacagc accgtcgtcg gcgtggtcac gctcaacgac
     4501 gtcatgagca cggtgatggg cgagctggtc acgcccgagg acgaggagcc gatcgtgctg
     4561 cgcgacgacg gctcctggct gatcgacggc cacacgccaa tggccgacgt gcagcgcgcg
     4621 ctgaacctgg acgagccgcc cagcgccgag gcctacgaca ccctggccgg ctttctgatg
     4681 gtgatgctgc gccgcgtgcc gcgccgcacc gacagcgtgc agtggggcgg ccaccgcttc
     4741 gaggtgatgg acgtggacaa ctaccggatc gaccaggtgc tggtcacccg cagtgagcgt
     4801 tcagccccgg agtgactact attttgatag ctgctggcgc aaaccagaca agcgtcaggc
     4861 cgacaaaacc catcttttct gggccccgaa cacggcgttc ggatactgcg gtcggccgcg
     4921 cgagccgttg tagcgcccca gcgccatgaa caggtcgccg cgctcacgct ccaggtagtg
     4981 gcgcaggatc acgcagccga agcgcaggtt ggtctgcatg tgaaacagct tgccggcgtc
     5041 gccgtcaccg atcacccggc tccagaacgg catcacctgc atgtagccac gcgcgcccac
     5101 gctggacacc gcgtacttgc gaaacgcgct ttcgacctgg atcagcccca gcaccagcga
     5161 ggtctcgagc ccggcgcgct gcgactcata ccagaccgtc tgcagaaatt cgatgcgggt
     5221 gttgaagtcc ttcttgcggc tgtagaggcg gtcgctcatg cccgacagcc agcgcaggta
     5281 gcgcaggcgc gcgtcggtgg aggcaaacac cggcagcggc ggcgcatcgt tggcgatggc
     5341 cgaggacagc gccgtgcgca ccgcgtcggc cagcggctcc tccagctggc cgccggccag
     5401 cgcgcgctcc tgaaaaatga gcgcgccggg cacggccatc aagcctcgga tgagctgcct
     5461 acgatccacg tccgacgcac ctccagaccg ctcggccccg gcgttcacac cgccagccgg
     5521 cccgtcacga aggcggcgat gccgtccacc ggcacccgag tggcggtgct gtcgcggcga
     5581 tgctggtatt ccacctgtcc gtccttgagg cctttgtcac cgatggtgac acggtgcggc
     5641 acaccgatca actcccagtc ggcaaacatc acacccggtc gctcgccgcg atcgtccagg
     5701 atcacgtcca ccccggcggc cagcagttcg gcgtacaggg cttcggcggc ggtcttgacg
     5761 gactcgctgc ggtccatgcc gaccgggcag atgaccaccg tgaagggggc gatggcatcg
     5821 ggccagatga tgccgcgcgc gtcgtggttc tgctcgatgg cggccgccgg caggcgcgtg
     5881 atgccgatgc cgtagcaacc catctcgaac ggtttgggct tgccgtcctc gtccaggaag
     5941 gtggcgttca tggcgcggct gtacttggtg cccaggtaga agatgtggcc gatctcgatg
     6001 ccgcgctcga tcgccagcac gccgcgcccg tcgggcgagg catcgccggc gaccacattg
     6061 cgcaggtcgg ccaccacatc cggctcgggc aggtcgcgcc cccagttcac gcctgtcagg
     6121 tgatggccct cggcattggc gccgcacacc cagtcggcca tcagcgccac ctcgcggtcg
     6181 gccaccacct tgaccggccg cttcaggccg atcgggccca ggtagccggg cggcgtgcca
     6241 aagtgcgcct gaatctcggg cacggtggca aagcgaaagc cggcgttcaa ccccggcacc
     6301 ttggcggcct tgatctcgtt catgtcgtgg tcgccgcgca gcagcagcag ccagatctgg
     6361 gtcttggcca cggcaccttg cgcgtccagc tcgtcggtgg ccagcaccag cgacttgacg
     6421 gtgcgcgcca gcggcaggcc cagcagttcg gccacttcgg cgcaggtggc cttgccgggc
     6481 gtgggcgtgt tcgtcagggc ctgcgcgggc gccgggcgcg ggccggcggg cgccagcgcc
     6541 tcggccttct ccatgttggc ggcgtagtcg ctgccggggc agtagacgat ggcgtcttcg
     6601 ccggtggcgg cgatgacctg gaattcctcg ctcagatcgc cgccgatggc cccggaatcg
     6661 gccgccaccg cgcggtagcg caggccaaag cggtcaaaga tggcgcggta ggcgcgcgcc
     6721 atggcctgat agctcctttt ggcgcccggt tcgtcgcggt cgaagctgta ggcgtccttc
     6781 atgatgaatt cgcgcccgcg catcaggccg aagcgcggcc ggcgctcgtc gcggaacttg
     6841 gtctggatct ggtacaggtt gcgcggcagc tgcttgtagc tgcgcaggtc ctggcgggcg
     6901 atgtcggtca ccgcctcctc gctggtgggc tgcaccacga agtcgcgctc gtggcggtcc
     6961 cggatgcgca tcagctccgg gcccatggtc tggaagcgcc cactttcact ccacagctcg
     7021 cccggctgaa ccaccggcat cacgcactcg accgcgccgg cgcggttcat ctcttcgcgc
     7081 acgatggcct cgaccttgcg gatcacgcgc aggcccatcg gcatgtaggt gtagatgccg
     7141 gcacccagct tcttgatcat gccggcacgg gtcatgagct ggtggctcac gatttcggcg
     7201 tcggccgggg cttctttcag ggtgctgatg tagaactggg aggctttcat ggggggtctg
     7261 gcgcagtcgg ggcgccgtgc aagcgggttt gtgcccactt gccgggcagc ctgcgccgtg
     7321 cataatggac acaattttta gattttgagg tttgattatg cttgaccggg aaggctttcg
     7381 cccgaacgtc ggcatcatcc tgctcaacca gaagaatcag gtattttggg gcaagcgcat
     7441 ccgcacccac tcctggcaat ttccgcaagg cggcattgac cggggcgaat cgcccgagca
     7501 ggccatgtac cgggaactgc acgaggaagt ggggctgcag ccggaacatg tgcgcatcgt
     7561 ggcccgaacc cgcgactggt tgcgctacga ggtgcccgac cggttcatcc gccgcgacgc
     7621 gcgcggccac tacaagggcc agaagcagat ctggtatctg ttgcagttga tcgcccagga
     7681 ctggcagctg aacctgcgcg ccaccagcca ccccgagttc gacgcctggc gctggaacga
     7741 ttactgggtg ccgctggacg tggtggtgga attcaagcgc ggcgtctacg aaatggcgct
     7801 gaccgagctg gcccgctatc tgccgcgcac cgagaaccgc aaccgttacc tgcgtggcgg
     7861 cgtgcgtgcc cgcgacggcg gcgaactgcc cgccatccag acccgcgatc gcctggcccg
     7921 tgccgcctcg gcggccatcg ccctgccccc tggcgcgacg ctggagggcg tgccccgtcc
     7981 gcctttcggc ccgccggaca agccatgagc cgccgcctgc gtcccccggg gcggcgcgcc
     8041 tggctggcct gcctgctggc ggcctgcgcc ggcctgttca gcgcgcccgg cgcggcccag
     8101 tatgccgacc tggatcgggc cgactggcag gaagacccgg tgccgccgcc accggcctac
     8161 agcctggagc ggctgctgga gatcgacatg cccaagacgg cgtcggtgcg catgggcatc
     8221 gacccgcaga ccctgagcgt caaccatcag agcggcgtgg tgcgctacgt ggtggtggcg
     8281 cgcggcacgt cggccgtcaa cgccagctac gaaggcatcc gttgcaccac cggcgagttc
     8341 cgcgtgtacg cgcgccaggt gcaagggggc gaatggacgc ccagcaccga cagcggctgg
     8401 aaaagcatgc gcggccagtc cagcgtgctg gtgcagcacc cgctgcgcct ggcccgcgat
     8461 ggcctttgcc tcggccccag cgcgcggcag acggtgtcgg aaatggtgcg cgagctcaag
     8521 accggcaacc gctcgctcta ctactgagac taagcggccg cgccacaccg gtgctgcaac
     8581 gtcaccgcct ggccgcgctc agcggatcag caccatgttg tcgcggtgca ccatctccgg
     8641 ctcggccatg tagcccagca gcaccgtgat atcgcccgac ggccgccggc acagcaggcg
     8701 cgcctcggcg ctggagtagt tggccagccc gcgcgccact tcggcgccgc gggcgtcgcg
     8761 gatggccacc acgtcgccac gcgcgaatgc gccctcgacc tgcgtcatgc cgatcggcag
     8821 caggctcttg ccggcgccac gcagcttggc cacggcgccg tcatcgatca ccagcgcccc
     8881 ccgcagctgc agatggtcgg ccatccactg cttgcgcgcc tgcagcttct gggtcggcgc
     8941 caccagcagg gtgccgatgc gctcgccccg cgacaggcgc agcagcacgt ccggctcccg
     9001 cccccaggcg atcacggtcg aggcgcccga acgcgccgcc cgcttggccg ccaggatctt
     9061 ggtcagcatg ccgcctttgc cgatgctgga gccggcgcca ccggccatgc gttccagggc
     9121 aggatcgccg gcccgcgcct catgcacgaa ctcggcctgc gggttggcgc gcgggtcggc
     9181 ggtgtacagg cccttctggt cggtcagaat gaccagcagg tcggcttcga tcaggttggc
     9241 caccagggcg cccagagtgt cgttgtcgcc gaccttgatc tcgtcggtga ccaccgtgtc
     9301 gttctcgttg atgaccggca gcacacggtg ctccagcagg gtcagcagcg tcgagcgggc
     9361 gttcaggtag cgctcgcggt cggccaggtc ggcgtgcgtc agcagcacct gggcgctgcg
     9421 caggccctgc tcgcgcagct tggtttcata catctgcgcc agccccatct ggccgaccgc
     9481 ggcagcggcc tgcagttcat tgacctcgct cggtcgctgg gtccagccca ggcgcttcat
     9541 gccctcggcg atggcgccgc tggacaccat gacgacttcg cgcggcaggt cggcctggcc
     9601 ggccagccgc gccatctggc gacaccattc gccgatggcg gtctcgtcca ggccgcgccc
     9661 ctcgttggtg accaggctgg agcccacctt gatgacgatg cgctgcgcct gacgcagcac
     9721 ctccgatacc gaattctgag tcatttgagg cgatagcact tgccaggcaa gcgccaacag
     9781 ctatcatttt tgatgtggtt cagcggcgtc ggcaaagcgc ggatcgggtg gctcggccgg
     9841 tgcgtcggcc acctgctgcg cacgtacgaa ctggtacacc gcctggatca agggctcgca
     9901 gccttcacgc gtgagggccg agatctgaaa caccggcccc ttccagcgca ggcggcgcac
     9961 gaactcgcgc acgcgggctt cgcgttcggc gggcggcacc atgtcgagct tgttgagcac
    10021 cagccagcgc ggcttggcgt gcagcgacgg gtcgtacttc ttcagctcgg ccacgatggc
    10081 gcgcgcctgc accaccggat ccacgccctc gtcgaacggc gcgatatcga cgatgtgcag
    10141 cagcaggcgc gtgcgttgca gatggcgcag gaactgatgg cccaggccgg cgccttcgct
    10201 ggcaccttcg atcaggcccg ggatgtcggc caccacgaag ctctgctccg gccccacgcg
    10261 caccacacca aggttggggt acagggtggt gaacgggtag tcggcgatct tcggccgtgc
    10321 gttgctgacg gcggcaatga aggtcgactt gccggcattg ggcatgccca gcaggccgac
    10381 gtcggccagc accttcagct ccagcttcag gctgcgtttt tcacctggcc agccgggtgt
    10441 cttctggcgt ggagcgcggt tggtcgagct cttgaaatgc aggttgccga aaccgccgtc
    10501 gccacccttg gccaccagca ccgcctggcc cggctgcagc aactcgtgca acacctcgcc
    10561 cgtgtcggcg tcgctgatga tggtgcccac cggcatgcgc agcgtgatgt cgtcgccggc
    10621 ggcgccgaac atgtcggagc ccatgccgtg ctggccgcgc ctggcctcgt gccggcgcgc
    10681 gtagcgaaaa tccaccaggg tgttcaggtt ggggtcggcc agcgcgaaca catgcccgcc
    10741 gcgcccgcca ttgccgccgt cggggccgcc gaactcgcgg aatttctcgc gccggaacga
    10801 ggagcagccg ttgccgccgt cgccggcagc cacgtcgatg aaggcttcgt caacgaattt
    10861 catgcggcgc tccggtcggt cgggcctgcg tcagaaacac caaagccccg ccggcgggcg
    10921 gggcttgtgg cctggacgca cgggaaaagt gcctcaagcg gcaggcgtca cctgcacgac
    10981 gtgcttgttg agcgcgccct tggtggcgaa cgacacctga ccgtccacca gggcgaacag
    11041 ggtgtggtcc ttgccgacgc cgacgttgga gccggcatgg aacttggtgc cgcgctggcg
    11101 cacgatgatc gagccggcgc tcaccgtctg gccgccaaac accttcacgc ccagcatctt
    11161 gggcttggaa tcgcggccgt ttcgcgtgga gccgccgcct tttttctgtg ccatggtgtg
    11221 ttactccttg gacgcgccgg cgccgttgat ggcgccgatc tcgagttcgg tatagccctg
    11281 gcgatggccc tggcgcttct gatagtgctt gcggcgacgc atcttgaaga tgcgcacctt
    11341 gtcgtgcttg ccgtgcgaca cgaccttggc cgtcacgctg gcacccgcca ccaggggagc
    11401 gccgaccgtc agatcgctgc cgttgccgac agcgagcacc tgatcgagcg tgatttcctg
    11461 gccaacgtcc gcagcaatct gttctacttt gattttttcg ccggcagcca cgcgatactg
    11521 cttgccaccg gtttttatga ccgcgtacat gatctcaagt cctttgatcg attgatccac
    11581 cagacggatt tctggcggaa ccgggcatta tagcgatctg cgcgaatgcc gcccagcccg
    11641 actccgtagg agcgcgccag gggcagccac cagccaccgc aaacctataa tcggcgccgc
    11701 tcgtggcgtg ccgcgcacgc cacgcccgaa gtcccacgcc cctgccgctc ttgaccacct
    11761 cccagaaccc gaccgcgcgt gcgctggccc tggctgccga cgacatgcga caggtcgacg
    11821 ccatcatttc gcgccggctc gattccgacg tgcccctggt gggccaggtg gcaggctaca
    11881 tcgtctcttc cggcggcaag cgcctgcggc cggtgctgct gctgctgatg tgccaggcgc
    11941 tcggctgcct ccacccgcag cgtcacacgc tggcggcggt ggtcgagttc atccacaccg
    12001 ccaccctgct gcacgacgac gtggtcgacg aatcgaccct gcggcgcggg cgcgccaccg
    12061 ccaaccagac gttcggcaac cctgccagcg tgctggtggg tgacttcctg tactcacgcg
    12121 ccttccagat gatggtcgaa agccacagca tgcgcgtcat gcaggtgctg gccgatgcca
    12181 ccaacgtcat cgccgagggc gaggtgctgc agctgatgaa catgcacgac gcctcgctgg
    12241 acgaggcggc ctacctgcgc gtcatccgct ccaagaccgc caaactgttc gaagccagct
    12301 gccgtctggc ggcgatcctg gccaacagcc cgcccgccct ggagcaggcc tgcgccgagt
    12361 atggccagtc gctgggcacc gccttccagg tcatcgacga cgtgctggac tacgacggcg
    12421 acagtggcga aatgggcaag aacctgggcg acgatctgag ggagggcaag gccacgctgc
    12481 cgctgatcct ggccatgcaa ctgggcaacg ccgacgaggc ccggatcgtg cgccaggcga
    12541 tcgagaccgg caacacgcag cagctgcccc gcatcatcga catcgtgcgc tgcacgggtg
    12601 cgctggacgc cacccgtgca gcggccgcgg cccaggcgga acgtgccatg caagcggcac
    12661 gcgcactgcc cgccaatgag gccaccaaca gtttgctaga attggcggct cagctattgc
    12721 aacggcgctc gtgagcagcg tgcagccggg caccatcggg gtgtagctta gcctggtaga
    12781 gcgctacgtt cgggacgtag aggccggagg ttcgaatcct ctcaccccga ccacttttcc
    12841 ccaccttttt cttttctggc agccaccctg ggcggtggtc cggaggcgaa tgtgtcgatt
    12901 gatgcacaca ttcaggggcg atcatcgttt acagaaattc accgatcagc gatgatcgaa
    12961 agccgttttt agccttttgg gtaacgtttt tccatggcca tcgccgatcc cgtctccaaa
    13021 accagcctgc ctgccgccct gcccggcgtg gggcgggcgt tggtgatggc tggcaagatc
    13081 acccagcaga ccgccgacga cgcgttccgc aaggccaagg cggccaatcg accgttcatt
    13141 gccgaactga tccagtcggg ggccatctcg gcggcggagc tggcgcacac catgtcgcag
    13201 gcgttttcgg cgccgctgct cgacgtcgac gcgctcgaca tcgagcacct gcccaagggg
    13261 ctgctcgacc ccaagctgtc ggcccaccac cgcatcgtgg cgctcaacaa gcgcggcagc
    13321 cgtctgatca tcgccacggc cgacccgtcc gaccacgaag cggcggaaaa ggtcaagttc
    13381 gccacccaga tgggcatcga gtgggtgatc ggcgagcacg acaagctgtc acgactgatc
    13441 gaggtgctcg gcaagtcgct gtccgacgcc atggacagca tcatcggcgg cgacttcgag
    13501 ttcgacgaag cctcgctcgc gtcggatgcc cagacggagg attccgacac cacgcagacc
    13561 gaggtcgagg atgcgccggt cgtcaagttc ctgcacaaga tgatggtcga cgccgtcaac
    13621 atgcgcgcct ccgacctgca cttcgagccc tacgagcacc agtaccgcgt gcggtttcgc
    13681 atcgacggcg aactgcgcga gatcgcctcg ccgcccaccg ccatcaagga aaagctggcc
    13741 tcgcgcatca aggtcctgtc gcgtctggac atctccgaaa agcgcgtgcc gcaggacggg
    13801 cgcatgaagc tcaagatggg tgccgaccgg gtgatcgact tccgggtcag cacgctgcct
    13861 acgctgtttg gcgaaaagat cgtgatccgt atcctggatc cgagcagcgc ccggctcggc
    13921 atcgacgccc tcggctacga gcccgaggaa aaagagcgcc tgctgaaggc catcgagcgc
    13981 ccctacggca tgatcctggt caccggcccc accggctcgg gcaagacggt gtcgctctac
    14041 acctgcctga acctgctcaa caagccgggc gtcaacatcg ccaccgccga agacccgtcg
    14101 gaaatcaacc tgccgggcgt caaccaggtc aacgtcaacg agcgggccgg actgaccttc
    14161 gcggtggcgc tgcgtgcctt cctgcgccag gacccggaca tcatcatggt cggtgaaatt
    14221 cgcgacctgg aaaccgccga catcgccatc aaggcggcgc agaccggtca cctggtgctc
    14281 tcgacgctgc acaccaacga cgcccccgcc acgctgacgc gcatgcgcaa catgggcatt
    14341 ccgcccttca acatcgcttc cagcgtgatc ctgatcacgg cgcagcggct ggcgcgacgc
    14401 ctgtgcacgc agtgcaaggc gccggtggac atcccgcgcg aaacactgag ggaagccgga
    14461 ttcgctgaca acgagctgga cggcagctgg acgccctacg gcccggtcgg ctgcgcggcc
    14521 tgtcacaacg gctacaaggg gcgcgtcggc atctacgaag tcatgccgat cagcgaagaa
    14581 atccagcgca tcatcctggc cgatggcagc gagctcgaga tcgccgagca ggcgcggcgc
    14641 gaaggcgtgc gctcgctgcg cgcggcggga ctgcacaagg tgaaaacggg agtgacgtca
    14701 ctggaggaag tgctgggcgt gaccaaccag tagagtcggc acccctgacc aggactgaac
    14761 acagggcatt tcatggcaac tcaagtcgct ggcgccaagg gcgtcaagga attcgtcttc
    14821 gagtgggaag gcaaggaccg caacggccgg gtggtgcgcg gcgaaacccg ggccgggggc
    14881 gagaaccagg tccaggccac gctgcgtcga cagggcatct tcgtcaccaa gatcaagaag
    14941 cggcgcatgc gcgcgggcaa gcgcatcaag cccaaggaca tcgccatctt cacgcgccag
    15001 ctggccacca tgatgaaggc cggcgtgccg ctgctgcagt cgttcgacat cgtggcccgc
    15061 ggcaacccca accccagcgt caccaagctg ctcaacgaca tccgcgccga cgtcgaaacc
    15121 ggcacctcgc tgtcggcggc gttccgcaag tacccgctgt acttcaacag cctgtactgc
    15181 aacctggtcg aagccggcga ggcggccggt atcctggaag acctgctgga tcggctggcc
    15241 acctacatgg agaagaccga ggccatcaag tccaagatca agtcggcgct gatgtacccg
    15301 atcgcggtga tcgtggtggc cttcgtggtg gtggccatca tcatgatttt cgtcattccg
    15361 gcgttcaagg aggtgttcag ctccttcggc gccgacctgc cggcgcccac gctgttcgtc
    15421 attgccatga gcgagttctt cgtccagtgg tggtggctga tcttcggcgg gctgggtttc
    15481 ggcgtctact tcttcatgca ggcctggaag cgcaacgaga agatgcagat gttcatggac
    15541 cgtctgctgc tgaagatccc gatcttcggt gaattgatcg acaaggccgt ggtggcgcgc
    15601 tggacgcgca cgctgtcgac catgttcgcc gccggcgtgc cgctggtcga atcgctcgac
    15661 tcggtgggcg gcgctgccgg caactcgctc tacaaggacg ccaccgacaa gatccagacc
    15721 gaagtcgcca ccggcaccag cctgaccgcg gccatgacca acgccaacgt gttccccagc
    15781 atggtgctgc agatgaccgc catcggcgag gaatccggcg cgctcgacca catgctgggc
    15841 aaggcagccg acttctacga ggccgaggtc gacgagatgg tggccggcct gtccagcctg
    15901 atggagccca tcatcatcgt cgtgctgggc accgtgatcg gcggcatcgt ggtgtcgatg
    15961 tacctgccga tcttcaagct cggccaggtg gtctgatgcc cgacggtctg gtggtcatgg
    16021 ccgccgtggg cggcctgttc ggtctgctcg tcggcagctt cctgaacgtg gtcatccacc
    16081 gcctgcctca gatgatggag cggcagtggg cggccgaatg tgccgaactg aacggccagc
    16141 ccgccgcccc gcaggcgccg ttcaacctgc tggtgccacg ctcgcgctgc ggccattgcg
    16201 ggcacccgat ccgctggtac gaaaacatcc ctgtgctgag ctacctggcg ctgcgcggca
    16261 agtgctcggc ctgcggcacg ccgatcggcc tgcgctaccc gctggtggag ctggcgaccg
    16321 gtctgctgtt cggctggtgc gtgtggcgct ggggctggag ctggacggcc gccgcctggt
    16381 gcctgttcgc ggccatgctg gtggcgctga cgctgatcga ctgggacacc accctgctgc
    16441 ccgacgacct gacgctgccg ctgctgtggc tgggcctgat cgccgccacc ctgggcctga
    16501 cgggcgtgcg cctgagcgac gcggtgtggg gcgcggtggc cggctacctg tcgctgtggc
    16561 tggtgtactg gggcttcaag ctggccaccg gcaaggaagg catgggcttt ggcgacttca
    16621 agctgttcgc cgcactgggc gcctggttcg gctggcaggc cctggtgccg atcatcctga
    16681 tggcctcggt catcggcgcg ctggtgggca tcggcatgaa gctgctcagc gcgctgcgcg
    16741 aaggcggggt ggtgcccttc gggccgtttc tggccctggg cggcatcacc gccatggtgc
    16801 tggggccgca gaccatcctg cgcgcggtcg gcctgtagcc atggcgctgc gcatcggcct
    16861 gaccgggggc atcggcagcg gcaagagcac ggtggcggcc atgctggtcg aacagggcgc
    16921 cgccctgatc gacgccgacg ccatctcgcg cgcctgcacc gccgccggtg gcgcggccct
    16981 gcccgccatc gcgcgcgaat tcggcccgca gctgatcgcg gccgatggcg cactggaccg
    17041 cgccgccatg cgcgagctgg tgttccgtga ccccggcgcg cgccaacggc tggaagccat
    17101 cgtccaccca ctggtcggcg ccgaaaccga gcgccaggcc cggcaggcgc tggcccaggg
    17161 gcgccgcctg ctggtgttcg acgtgccgct gctggtcgag tcgatcgagc gctggcgcca
    17221 gcgggtggat cgcatcgtgg tggtcgactg cgaacccgag acccagatcg cccgcgtgat
    17281 ggcgcgcaac cagctgccgc gggcccaggt cgagcagatc ctggcggcgc aggccagccg
    17341 cgcacaacgg ctggcggtgg ccgatgcggt gatcttcaac ggtgccgacg tgacgctgca
    17401 ggcgctgcgc gcccaggtgc gtcaactggt cgatgccttc gggatatgat gcgggcgacc
    17461 ctgacaaggc gccacgcgtg atcctgtacg agtacccttt caacgaacgc atccgcacct
    17521 accttcggct ggagcacctg ttcggccgtc tggcccagct gatgccgcgc gacgcgccgc
    17581 tggaccatca cttcgccctc atcaccctgt tcgaggcact ggacgtgtgt gcccgttcgg
    17641 acctgaagtc cgacgtgctc aaggacctgg acaagcagaa gacccatctg gccagctttc
    17701 gcggcaaccc ggcagtctcc gaggcctcgc tggagaccat gctggccagc ctggaagagg
    17761 cgtttgaaca gctcaaccag cagcacggca agccgggcca cgagctgacc gacaacgagt
    17821 ggctgatggc catccgcagc cgcgccgtca ttcctggcgg cacctgcggc ttcgacctgc
    17881 cggcctacca cgcctggcag cacggccagg cggcggcgcg gcgcaccgag ctgcaccgct
    17941 gggccgccac gctggcgccg atggcgcgcg ccatcgcgct gctgctgcag atcctgcgcg
    18001 attccggcgc gccacagaag gtgatggcca gcgccggcca gtttcagcag acgctgccgc
    18061 aaggccggag ctttcagctg ctgcgcctgc gcatcgaggc atcgacgcag ctgatccccg
    18121 agatcagcgg caaccgcctg atggtgtcgg tgcgcctgat gcggcaggac gccgacggca
    18181 agctgcagct ggcgcgcgag gacgcggcct tcgagctggc actgtgcctc tgatcccgtg
    18241 ttgcgcccgc cccatgcccc cgcccgacgc cgccccgccc agcctgccgc cccgcagcgt
    18301 gcgctgccct gcctgcggtg gcgtcagccg ctacgccgcc gacaacccgt atcgcccgtt
    18361 ctgcagcgcg cgctgccgcc agcacgacct gggcgcctgg gccagcgaga gctttcgcct
    18421 cgaggcccag gaagcggacg gcaccgacca agctgcaccc gcccatccat cggcaccccg
    18481 gtttccgcaa tgaacagggc tccggcgctt tcctgacaag cgccgccagc tatataaaca
    18541 atagcattct caggccgtgc gctcgacctg cagccaatcc agcaccggca gagcgcctgg
    18601 cagcaccggc gccacgcgga ccggcagccg ctcccaggca aaggcctggc cctcgcgcat
    18661 ctgcagtgtg ccgccccacc ggtacagccg gcagaaatgc agccgcacca gcgcatgcgg
    18721 atagtccacc agctgctcgc gccagcgccg cacgtcggcc gggtcgatgt ccagacccag
    18781 ctcctcgtgc agctcgcgcg ccagcgccgc ctcgacgctc tcgcccggct ccagcttgcc
    18841 ccccggaaat tcccaatagc ccgcatacac cttgccggcc gggcgcgagg tcagcagaaa
    18901 gcgcccgtcc ggccgttgca gcacgccgac cgccacgtcg accaccgacc gcgcaccggc
    18961 ctgcgcgcgc cgcaggtggc cgtcggcggt cagcacgggg ttcggcactg ggctcatggc
    19021 gtgaccggtg cagccgccgg cggctcaacc tagatacggc cgttggaggc gcgtgccagc
    19081 gccgtgatcg gtgtgccagg caagggtgag cgccgcggct ccaaaggcgc ctgcg
//
LOCUS       DAOOOG010000031        18827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_3005_length_18827_cov_31.489239, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000031 DAOOOG010000000
VERSION     DAOOOG010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 18827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18827
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3005_length_18827_cov_31.489239"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PK242_04180"
     CDS             <1..653
                     /locus_tag="PK242_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HOZ93247.1"
                     /translation="LAPHSLTLTMVGASLLWVGWFGFNAGSAGAANAIAGLAFVNTVL
                     ATAAATLSWIALEAMHKGKASMLGAASGAVAGLVAVTPAAGFVGPMGAIVMGVIAGAC
                     GLWGVSGLKRLLRVDDAFDVFGVHGVCGIVGAILTGVFAAQGLGGTGGLTPDSFSMGA
                     QVWIQIKSVLFTIVWSAVVSLIAFKIADLVLGLRVSEEDERQGLDISSHGETAYSR"
     gene            754..1824
                     /gene="glcE"
                     /locus_tag="PK242_04185"
     CDS             754..1824
                     /gene="glcE"
                     /locus_tag="PK242_04185"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HOZ93248.1"
                     /translation="MEAALQSLAAKITQAAARGRPLRIRGGGTKDFYGQQLLGELLDA
                     RPLAGVLAYEPSELVVTVGAGTRLRELEALLAEAGQHLPFEPPRFGVAGTVGGAVAAG
                     LSGPARASVGAARDFVLGAQLINGRGERLNFGGQVMKNVAGYDISRLLAGSLGTLGLI
                     TQLSLKVLPRAPAEATLVFALSETSARQQLNRWCGQPLPLSASAWADGQLWVRLRGAQ
                     AALASACRQMGGERLDEPRAQRQWDALRDQTLPFFRLAPDEALWRVSVPDTASPLNLG
                     PTLVEWHGAQRWLRLPPAEAPRVREVAARLGGHATLFRGGDGVTPVFTPLTPPLARIH
                     QALKREFDPAGIFCPGRLTPDF"
     gene            1824..3065
                     /gene="glcF"
                     /locus_tag="PK242_04190"
     CDS             1824..3065
                     /gene="glcF"
                     /locus_tag="PK242_04190"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HOZ93249.1"
                     /translation="MQTALAPEYQGTALGQEAEAILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGHAPTRETQLHLDRCLTCLNCESTCPSGVQYGHLIDIGRQVV
                     EAKVARPPAEARTRALLRRGLSSPLFGPAMRLGQSLRGLLPATLRAKVPAPRPAGAWP
                     RRTHARKVLLLEGCVQPSMAPGINAATARVLDAAGIECVLARGQGCCGAIKYHLADGA
                     GGLAQMRANIDAWWPHIERGAVEAIVINASGCGAMVKDYGHLLRHEPPYAERAARVSA
                     MARDVSELLPDLVAALRGRVRPPAEVLAWHAPCTLQHGQRLRGGVEQHLGALGFRLRP
                     AAQEAHLCCGSAGTYSVLQPELSLRLRERKLGQLLDEGAADQPAAILSANIGCITHLQ
                     AGTEVPVRHWIELLDQALHAT"
     gene            3148..4746
                     /locus_tag="PK242_04195"
     CDS             3148..4746
                     /locus_tag="PK242_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HOZ93250.1"
                     /translation="MTGIQDWTLPYASRRSPVLGRQVVSTSQPLAAQAGLRMLLAGGN
                     AVDAALAAAMALTVVEPSGCGIGSDAFAIVWDGQALHGLNASGRAPRAWTPDYFARLG
                     GIPDKGWNAVTVPGAVSGWIALWRRFGRLPLAQLAQPAIDYARHGFPVSPTIATLWTL
                     GAERLGAQPGFAECFLPAGRPPRAGELFRSEAHARTLEDIVATEGESFYRGALAARMA
                     AHARACGGAMTEDDLGAHQADWVGTLAQRFGDSVVHEIPPNGQGIAALMALGMLDELG
                     IGDAPVDSADSLHLQIEAMKLALADVYHYNADIDHMALAPGQLLERGYLRQRAALIDR
                     QRAGEPTWGTPRPGGTVYLAAADAQGMMVSFIQSNYMGFGSGVVVPATGISLQNRGHG
                     FSSATGHANQVGPGKRPSHTIIPAFAMHADGTPQMAFGVMGGPMQHQGHVQMALRVLR
                     HGQNPQAAADAPRWRVTGGRGVALEPAVDAGVVQALRARGHDVRLESGHGVFAFGGAQ
                     LVLRQDGHYVAGSDPRKDGGAVAF"
     gene            4935..5945
                     /locus_tag="PK242_04200"
     CDS             4935..5945
                     /locus_tag="PK242_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93251.1"
                     /translation="MRHPYTLIGLTPSERALLESLFALERDTSGAMVPTPDPREAHLI
                     VVNGDDHTLVEQLRQDNPRALIVLAGHPAGAPVTGLPVLRRPFDMPGVIEVLSGLDWP
                     AGLPCVGAGEFDRTFVSSAPTSSQLDTGAPPAVDPQAFAPTTASAPLAPAPQGRAVAV
                     PDRAPVSSRAAWARSELAPPPWSAAAAAAPGGFVDDREAEVLVVTGALGERSLTLPRG
                     IRRLGLRVRLLDGPAAVLAALDQGAASFVFLDQLSLGEQLLPLARALAARRAGNGAPP
                     HVVVVARRGTPLDRLRARLAGCHWMQVPIDGPRLVAFFARRGLRPRDGVGPAFSSTPD
                     DQ"
     gene            complement(6031..6933)
                     /locus_tag="PK242_04205"
     CDS             complement(6031..6933)
                     /locus_tag="PK242_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ93252.1"
                     /translation="MAAAHPPSALLPALALLGNALVWGLSWIAFKALHQHGLHPLWST
                     AVVFGVALLALLAWQPASLRAFARHPQLLVLAAAAGVTNIGFNWAVTLGDVVRVTLLF
                     YLMPVWSVGLAWWLLGERPTRAALLRLVLALAGLVLVLHRPGAPWPWPRGAADQLALL
                     GGAGFALTNVLLRRWRATPPPARVLAMFGGGFATATGLALAGLAGGLSPLALSTHGLA
                     WLPWVLGLTLAFLAGNLALQYGAARLPARVTALIMLAEVVFASASAVLLGAATPTRWT
                     WAGGVLILLAALLSILPMPRGRAG"
     gene            complement(7139..8419)
                     /gene="lplT"
                     /locus_tag="PK242_04210"
     CDS             complement(7139..8419)
                     /gene="lplT"
                     /locus_tag="PK242_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HOZ93253.1"
                     /translation="MRRGFYTIMAAQFFSSLADNALFVAAVELLRGSGAAEWQRAALV
                     PMFALFYVVLAPWVGAFADSRPKGRVMFLSNAIKIVGCLAMLFGGHPLLAYAIVGLGA
                     AAYSPAKYGILTELLPPSQLVRANGWIEGLTIASIILGVLLGGQLVGPQLAPRLLGLD
                     LPGLHSGIDTPAEAAIASLVLVYAIAAAINLRIPHTGVRMRPLPARTLSLLPDFWRCN
                     ARLWRDKLGQISLATTTLFWGVSGNMRYIVLAWAAAALHYSTTQAAGLVGVVAIGTAV
                     GAVLASMRVRLDRAIGVMPLGVLMGALVVLMVFIDRLWLAVPFLIFMGALGGYIVVPM
                     NALLQHRGHNLMGSGRSIAVQNFNEQACILGLGALYSASAGAGLSAFGAIALFGAVVI
                     AFMLLIQRWHRHNLRRYPGKVERLLNIARRDRGH"
     gene            8549..9628
                     /gene="alr"
                     /locus_tag="PK242_04215"
     CDS             8549..9628
                     /gene="alr"
                     /locus_tag="PK242_04215"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOZ93254.1"
                     /translation="MPRPIQATIHTGALRHNLERLRQAAPGAQLWAVVKANAYGHGIE
                     RVFEGLRGADGFALLDLAEAERVRALGWRGPILLLEGVFEPRDLELCSRLSLWHAVHD
                     DAQIDWLAMHKTHQPHHVFLKLNSGMNRLGFAPERLRAAHARLSALPQVGEISLMTHF
                     SDADGARGIAAQWAVFQRVTEGLPGERSVCNSAATLRHAQDAAVRADWVRPGIAAYGG
                     SPDFPAHDAAHWGLQPAMTLRSRLIATGSLQAGDSIGYGSTFTAERPMRVGVVACGYA
                     DGYPRHAPTGTPVLVDGVRTRLLGRVSMDMLSVDLEPVPQAGPGSAVTLWGQGLPIDE
                     VAHAAGTIGYELMCALAPRVPVQVD"
     gene            complement(9771..10529)
                     /locus_tag="PK242_04220"
     CDS             complement(9771..10529)
                     /locus_tag="PK242_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOZ93255.1"
                     /translation="MDLLLVSLASLLAGGIDAIVGGGGLVLVPALFAVYPNAAPATLF
                     GTNKSASVWGTGIATFQYARRVQMRWAVLGPAALAALAGSFLGAWAVTLIDPTFLRRA
                     LPLILLAVLLYTLAKKDLGRTHAPRHERGRETLLACAIGAVIGGYDGFFGPGTGSFFI
                     FLFVRLLGYDFLHASAAAKLLNVATNVAAIALFAAKGHVWWQVGLLMAAANVTGSLIG
                     TRLALRHGAGFVRGVFIVVVGALILKTGYDAFLR"
     gene            complement(10883..12235)
                     /gene="argG"
                     /locus_tag="PK242_04225"
     CDS             complement(10883..12235)
                     /gene="argG"
                     /locus_tag="PK242_04225"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOZ93256.1"
                     /translation="MSHTILQSLPAGQKVGIAFSGGLDTSAALLWMRQKGAVPYAYTA
                     NLGQPDESDYDAIPRKALEYGAEKARLVDCRTQLANEGIAALQCGAFHIQTAGATYYN
                     TTPLGRAVTGTMLVNAMKEDDVNIWGDGSTYKGNDIERFYRYGLLANPALKIYKPWLD
                     QRFIDELGGRKEMSEFMQKAGFDYKMSAEKAYSTDSNMLGATHEAKDLEELSSGIRIV
                     NPIMGVAFWKPEVEVKAEEVRVRFEEGRPVALNGREIADPVELFLEANRIGGRHGLGM
                     CDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQ
                     GRWFDPQAIMLRETAQRWVAGAVTGEVTLELRRGNDYSILNTESPNLTYAPERLSMEK
                     VENAPFTPLDRIGQLTMRNLDITDTRGKLAVYSRSGLLQLGEGSMLPQLGHDSSASRK
                     "
     gene            complement(12426..12737)
                     /locus_tag="PK242_04230"
     CDS             complement(12426..12737)
                     /locus_tag="PK242_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOZ93257.1"
                     /translation="MPSRPTAIPAELVELLDDAALSLHELARACAMSPEWVHAHVQAG
                     VLQPLGGGASTEWRFASLSLRRARRIAQLEQVYDADPQLAALTADLMEEVIALRQRLD
                     G"
     gene            complement(12877..13860)
                     /locus_tag="PK242_04235"
     CDS             complement(12877..13860)
                     /locus_tag="PK242_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOZ93258.1"
                     /translation="MQFKDYYQILGVSHSASPEDIKKAYRKLARRYHPDVSKEPDAAR
                     RMAEVNEAHTVLSDPEKRAAYDSVGAQAWAAGARSGDDVRPPPGWNTGYEFSDGAAPG
                     FGPHGEGGEFSEFFEQLFGRARAGARGARPGAGAQAPLRGQDHHARIELELIDAYRGA
                     ERAIHLRGAHLDAQGQVRPQERTLQVRIPQGVKPGQLIRLAGQGEPGWHGGPAGDLFL
                     EVQFRPDARWRIDGRDVTQRLRVTPWEAALGGAVQVATPDGKTVEVGVPAGSGSGRKL
                     RLKGRGIPSASAPGDLLLELELAVPGAVTDAQKAAWARLAEAYPGFDPRGA"
     gene            complement(14033..14941)
                     /locus_tag="PK242_04240"
     CDS             complement(14033..14941)
                     /locus_tag="PK242_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ93259.1"
                     /translation="MSARREHLDGRAIAILLVCCLFWGVQQVMVKATLPELAPVFQAA
                     LRFVGATALLWLWCRWRGVPLFQRDGTLGAGLLAGALFAGEFAAMYLGLQYTTASRLT
                     VFLYTSPFWVALVLPWLVPAERLHRLQWLGLACAFAAVLFALREGFAQGGAGPTWRGD
                     LLALAAGALWGLTTVSIRATRLVRVAPEKMLFYQVAVSAVVLPLLSLALGEPWRWRWS
                     AFAASSLALQTVVGAFASYLAWMWLLAHYPATRMSAFSFLTPIFALMAGALWLGEPIT
                     VSLVVALALVALGMLLVNRRAPARSD"
     gene            complement(15152..15661)
                     /locus_tag="PK242_04245"
     CDS             complement(15152..15661)
                     /locus_tag="PK242_04245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOZ93260.1"
                     /translation="MPHALRAHQPAFSAQEFRAALGMFATGVTIVSARSADGALVGLT
                     ANSFNSVSLDPPLVAWSLGLRSLSLHAFRRGSHYAINVLAAEQQELALRFAAPVGDRW
                     AGVAWHPGVAGAPLIEGAVAHFECFNRSRHDEGDHVLFIGQVEHCAHRPGAAPLLFHG
                     GRFYTEHPL"
     gene            15769..16563
                     /locus_tag="PK242_04250"
     CDS             15769..16563
                     /locus_tag="PK242_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOZ93261.1"
                     /translation="MALLPSFATLGHGPPVVLLHGSGGGARSFAPQLESLASLGFRAI
                     AWDMPGYGQSAPIEPYGFKGLAERGAALIEALAPLTGGAPVALVGHGLGGMVAQELVL
                     RRPDLVRQLVLVASVAAVSADDGYGRHVAQALAWLDEGRDMGWIAAERVPRLIGPGAL
                     ASGVRLATHCQATVPAPTWRRALLALAGFDRRAALARIAVPTLLVAGELDPLAPPPVL
                     RAMAAQISGAHSERLAHTGHLPQLERPQAFDRLLLPFLRHARAWRH"
     gene            complement(16637..17059)
                     /locus_tag="PK242_04255"
     CDS             complement(16637..17059)
                     /locus_tag="PK242_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ93262.1"
                     /translation="MKILLAVDGSAFTKKMLAYLTTHLDLFSGDIEYTALTVHGALPP
                     RARAAVGREIVETYYAEEAAKVLDPVVKFLTRHQLNVKTLSKVGNAGELIAKTAESGK
                     FDLIVMGSRGHGALGGLVMGSVATKVLASCEVPVLLVR"
     gene            complement(17286..17414)
                     /locus_tag="PK242_04260"
                     /pseudo
     CDS             complement(17286..17414)
                     /locus_tag="PK242_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            17579..17767
                     /locus_tag="PK242_04265"
     CDS             17579..17767
                     /locus_tag="PK242_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93263.1"
                     /translation="MTMWNVWIRHLTTLFAPLTRAPRHGPGVPASLLERADACAGRDP
                     RGAADLRAAALNYLGVVR"
     gene            17802..18080
                     /locus_tag="PK242_04270"
     CDS             17802..18080
                     /locus_tag="PK242_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93264.1"
                     /translation="MSTTSLTLGRTAVREPQFEPARLDHWLPRPQTGGPSMSQLLRAR
                     WNQLRQRLAQARSEREIQALADLDHRVQRDLQTARDLAEWKVPHRPFV"
     gene            complement(18228..>18827)
                     /locus_tag="PK242_04275"
     CDS             complement(18228..>18827)
                     /locus_tag="PK242_04275"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HOZ93265.1"
                     /translation="IDAFIFGGRRSTTVPLVTEARDWQEGVYMAATMGSETTAAAFGA
                     QGVVRRDPFAMLPFCGYNMADYFGHWLKLGQRLQAAGARLPAIYCVNWFRKDAAGKFV
                     WPGYGENMRVLKWMIDRVEGRAAGQPTMFGVAPRHDEINWSGLSFSPAQFDTVTRIDA
                     DVWAQEFALHDELFTKLAQGLPPELLATRQALAQRLEAA"
BASE COUNT         2649 a         6794 c         6818 g         2566 t
ORIGIN      
        1 cgctggcccc gcacagcctg acgctgacca tggtgggcgc ctcgctgctg tgggtgggct
       61 ggttcggctt caacgccggc tcggccggtg ccgccaacgc catcgccggt ctggccttcg
      121 tcaacaccgt gctggccacg gcggccgcca cgctgagctg gatcgcgctg gaggcgatgc
      181 acaagggcaa ggcctcgatg ctgggcgcgg cctcgggcgc ggtggccggc ctggtggccg
      241 tgacgccggc ggccggcttc gtcggcccga tgggcgccat cgtcatgggc gtgatcgccg
      301 gcgcctgcgg cctgtggggc gtcagcggcc tcaagcgcct gctgcgggtg gacgacgcct
      361 tcgacgtgtt cggcgtgcac ggcgtgtgcg gcatcgtcgg cgccatcctg accggcgtgt
      421 tcgcggccca gggcctgggc ggcaccggcg gcctgacgcc cgacagcttc agcatgggcg
      481 cgcaggtgtg gatccagatc aagagcgtgc tgttcaccat cgtctggtcg gccgtggtgt
      541 cgctgatcgc cttcaagatc gccgacctgg tgctgggcct gcgcgtcagc gaggaagacg
      601 agcgccaggg cctggacatc agcagccacg gcgagaccgc ctacagccgc tgacggcgcc
      661 cgcttctcca aggtcttcat ggcccgcccg ccggcgggcc tttttttcgt cccgggcgcg
      721 gcccgcgcag cgggctgccg ctaccatgac ggcatggaag ccgcattgca gtccctggcc
      781 gcaaagatca cgcaggccgc cgcgcgcggc cgtccgctgc gcatccgcgg cggtggcacc
      841 aaggatttct atggccagca gctgctcggc gagttgctgg acgcgcgccc gctggccggc
      901 gtgctggcct acgaaccgag cgagctggtg gtcaccgtgg gggccggcac gcgcctgcgc
      961 gagctggaag cgctgctggc cgaagccggc cagcacctgc ccttcgagcc accacgcttt
     1021 ggcgtcgccg gcaccgtcgg cggcgcggtg gcggccgggc tgtcggggcc ggcacgcgcc
     1081 agcgtcggcg cggcgcgcga cttcgtgctg ggcgcgcaac tgatcaacgg ccggggcgag
     1141 cggctgaact tcggcggcca ggtgatgaag aacgtggccg gctacgacat cagccggctg
     1201 ctggccggct cgctgggcac cctggggctg atcacccagc tcagcctgaa ggtgctgccg
     1261 cgcgcgccgg ccgaggccac gctggtgttc gcgctgtcgg aaaccagcgc ccgccagcag
     1321 ctcaaccgct ggtgcggtca gccgctgccg ctgagcgcca gcgcctgggc cgacggccag
     1381 ctgtgggtgc gcctgcgcgg cgcccaggcg gcgctggcca gcgcctgccg gcagatgggc
     1441 ggcgagcggc tggacgagcc gcgcgcccag cgccagtggg acgcgctgcg cgaccagacg
     1501 ctgccgttct ttcgcctggc gccggacgag gcgctgtggc gcgtgtcggt gcccgatacc
     1561 gccagcccgc tcaacctggg gccgacgctg gtcgagtggc atggcgcgca gcgctggctg
     1621 cgcctgccgc cagccgaggc gccgcgcgtg cgcgaggtgg cggcgcggct gggcggccac
     1681 gccacgctgt ttcgcggcgg cgacggcgtc accccggtgt tcaccccgct gacgccgccg
     1741 ctggcgcgca tacaccaggc gctcaagcgc gagttcgacc cggccggcat cttctgtccc
     1801 ggccgcctga ccccggattt ctgatgcaga ccgcactggc ccccgaatac caaggcaccg
     1861 cactcggcca ggaggccgag gccatcctgc gcaaatgcgt gcactgcggc ttctgcaccg
     1921 ccacctgccc gacctaccag ctgctgggcg acgagctgga cggcccgcgc gggcgcatct
     1981 acctgatcaa gcaggtgctg gaaggccacg ccccgacgcg cgaaacccag ctgcacctgg
     2041 accgctgcct gacctgcctg aactgtgagt ccacctgccc cagcggcgtg cagtacggcc
     2101 acctgatcga catcggccgc caggtggtgg aagccaaggt ggcgcgcccg ccggccgagg
     2161 cgcgcacccg cgccctgctg cgccgcggcc tgagctcgcc gctgttcggc cccgccatgc
     2221 gcctggggca gtcgctgcgc ggcctgctgc cggccacgct gcgcgccaag gtgccggcgc
     2281 cgcggccggc cggcgcctgg ccgcgccgca cgcacgcgcg caaggtgctg ctgctggaag
     2341 gctgcgtgca gccatccatg gcgccgggca tcaacgccgc caccgcgcgc gtgctggacg
     2401 ccgccggcat cgaatgcgtg ctggcgcgcg gccagggctg ctgtggcgcc atcaagtacc
     2461 acctggccga cggcgccggc gggctggcgc agatgcgcgc caacatcgac gcctggtggc
     2521 cacacatcga gcgcggcgcg gtcgaggcca tcgtcatcaa cgcctccggc tgcggcgcca
     2581 tggtcaagga ctacggtcac ctgctgcgcc acgagccacc gtatgccgag cgcgccgcgc
     2641 gcgtatcggc catggcgcgc gacgtcagcg agctgctgcc cgatctggtg gcggcgctgc
     2701 gcggccgcgt gcgcccgccg gccgaggtgc tggcctggca cgccccgtgc acgctgcagc
     2761 atggccagcg gctgcgcggc ggcgtcgagc agcacctggg cgcgctgggc tttcggctgc
     2821 gcccggcggc gcaagaggcg cacctgtgct gcggctcggc cggcacctat tcggtgctgc
     2881 agcccgagct gtcgctgcgg ctgcgcgagc gcaagctggg ccagctgctg gatgagggcg
     2941 cggccgacca gccggcggcc atcctgtcgg ccaacatcgg ctgcatcacc cacctgcagg
     3001 ccggcaccga ggtgccggtg cggcactgga tcgagctgct cgaccaggcc ctgcatgcta
     3061 cttaattgat agctgttggc gcttgtctgt cgggcgccag agccgttttc ttgctcttgt
     3121 ctcaagcgcc ttaccaagga ttcgcccatg accggcattc aggactggac cctgccctac
     3181 gcctcgcgcc gctcgccggt gctggggcgg caggtggtca gcacctcgca gccgctggcc
     3241 gcccaggccg ggctgcgcat gctgctggcc ggcggcaacg cggtggacgc agcgctggcc
     3301 gccgccatgg cgctgacggt ggtcgaaccc agcggttgcg gcatcggcag cgacgccttc
     3361 gccatcgtct gggacggcca ggccctgcac ggcctgaacg cttcgggccg cgcgccccgg
     3421 gcctggacgc cggactactt tgcgcgcctg ggcggcatcc ccgacaaggg ctggaacgcc
     3481 gtcacggtgc cgggcgcggt gtcgggctgg atcgcgctgt ggcgccgctt cgggcggctg
     3541 ccgctggcgc agctggcgca gccggcgatc gactacgcgc gccacggctt tccggtctcg
     3601 cccaccatcg ccacgctgtg gacgctgggc gccgagcggc tgggcgccca gcccggcttt
     3661 gccgaatgct ttctgcccgc cggccggccg ccgcgggccg gcgagctgtt ccgcagcgag
     3721 gcccatgcgc gcacgctgga ggacatcgtc gccaccgagg gcgagtcgtt ctaccgtggc
     3781 gcgctggccg cgcgcatggc cgcccacgcg cgcgcctgcg gcggcgccat gaccgaggac
     3841 gacctgggcg cgcaccaggc cgactgggtg ggcacgctgg cgcagcgctt cggcgactcg
     3901 gtggtgcacg agatcccgcc caacggccag ggcatcgccg ccctgatggc gctgggcatg
     3961 ctggacgagc tgggcatcgg tgacgcgccg gtggacagcg ccgacagcct gcacctgcag
     4021 atcgaggcca tgaagctggc gctggccgat gtctatcact acaacgccga catcgaccac
     4081 atggcgctgg cgcccgggca gctgctggag cgcggctacc tgcgccagcg cgccgccctg
     4141 atcgaccgcc agcgcgccgg cgagccgacc tggggcacgc cgcgccccgg cggcacggtg
     4201 tacctggcgg cggccgacgc ccagggcatg atggtgtcct tcatccagtc caactacatg
     4261 ggcttcggct ccggcgtggt ggtgcccgcc accggcatca gcctgcagaa ccgcggccac
     4321 ggcttcagca gcgccaccgg ccacgccaac caggtggggc cgggcaagcg cccctcgcac
     4381 accatcatcc cggccttcgc catgcacgcc gacggcacgc cgcagatggc cttcggcgtc
     4441 atgggcgggc cgatgcagca ccagggccat gtgcagatgg cgctgcgcgt gctgcgccac
     4501 ggccagaacc cgcaggccgc cgccgacgcg ccgcgctggc gcgtcaccgg cgggcgcggc
     4561 gtggcgctgg agccggcggt ggacgccggc gtggtgcagg cgctgcgcgc gcgcgggcac
     4621 gacgtccgcc tggaaagcgg gcacggcgtg ttcgccttcg gcggtgccca gctggtgctg
     4681 cgccaggacg ggcactacgt ggccggctcc gacccgcgca aggacggcgg cgcggtggcg
     4741 ttctgaagca cggcgcgccg gcggcgtgca aaagtcacgt cggcgcggcg cgaccgccac
     4801 gccgaggccg agcaagcacg catcggccgc ctgggtgagg cgcaccgccc cagcagtgcc
     4861 gccgcgccgc gccgcggcgc cccacaatcc cgccgcccgc caccgtggcg ggccaacccc
     4921 tgcatcgcca ccgcatgcgc cacccctaca ccctgatcgg tctgacgccg tccgaacgcg
     4981 ccctgctgga gtcgctgttc gccctcgagc gggacaccag cggggccatg gtgccgacgc
     5041 cggatccgcg cgaggcccac ctgatcgtgg tcaacggcga cgaccacacc ctggtcgagc
     5101 aactgcgcca ggacaacccg cgggcgctga tcgtgctggc cggccacccc gcgggcgcgc
     5161 cggtcaccgg actgccggtg ctgcgccgcc cgttcgacat gccgggcgtg atcgaagtgc
     5221 tgagcggcct ggactggccg gccgggctgc cgtgcgtggg ggcgggcgag ttcgaccgca
     5281 ccttcgtctc ttcggcgccg accagcagcc agctcgacac cggcgccccg ccggcggtcg
     5341 acccccaggc gtttgcgccc accaccgctt cggcgccgct ggcgcctgcg ccgcaaggtc
     5401 gcgccgtagc cgtacccgac cgggccccgg tgtcctcgcg cgccgcctgg gcccgcagcg
     5461 agctggcgcc gccgccctgg agcgccgcag cagccgctgc gccaggcggc ttcgtcgacg
     5521 accgcgaggc cgaggtgctg gtggtcaccg gcgcactcgg cgagcgctcg ctcaccctgc
     5581 cgcgtggcat ccggcgcctg ggcttgcggg tgcggctgct ggacggcccc gcggccgtcc
     5641 tggcggccct ggaccagggc gcggcatcct ttgtcttcct cgaccagctg tcgctgggcg
     5701 agcagctgct gccgctggcg cgcgcgctgg cggcgcggcg tgccggcaac ggtgcgccgc
     5761 cgcacgtggt ggtggtggcg cggcgcggca ccccgctcga tcgcctgcgc gcccgcctgg
     5821 ccggctgcca ctggatgcag gtgccgatcg acgggccgcg gctggtggcc ttcttcgcgc
     5881 gccgcggcct gaggccgcgc gacggcgtcg ggcctgcatt cagctcaacg cccgatgacc
     5941 aatgacgcat gacgccttcg tcatcgaagc gcagcgaggt cattcgtcat tgggcatggc
     6001 tcagggcttg agcgccaggc cgcaccaccg tcaacccgct cgcccacgcg gcatcggcag
     6061 gatcgacagc agcgccgcca gcaggatcag cacgccgccg gcccaggtcc agcgggtggg
     6121 cgtggcggcg cccagcagca ccgcggaggc gctggcgaac accacctcgg ccagcatgat
     6181 gagcgccgtc acccgtgccg gcagccgggc ggcgccgtac tgcagggcca ggttgccggc
     6241 cagaaaggcc agcgtcaggc ccagcaccca gggcagccag gccagcccgt gcgtgctcag
     6301 ggccagcggc gacaggccgc cggccaggcc ggccagcgcc agtccggtgg cggtggcgaa
     6361 accgccgccg aacatggcca gcacgcgcgc cggcggcggg gtggcgcgcc agcgccgcag
     6421 cagcacgttg gtgagcgcaa aacccgcccc gcccagcagg gccagctggt cggccgcgcc
     6481 gcgcggccac ggccagggcg cgccgggccg atgcagcacc agcaccagcc cggccagggc
     6541 gagcaccagc cgcagcagcg ccgcgcgcgt gggccgctcg cccagcagcc accaggccag
     6601 accgaccgac cacaccggca tcaggtagaa cagcagggtc acgcgcacca cgtcgcccag
     6661 cgtcaccgcc cagttgaagc cgatgttggt cacccccgcc gcggccgcca gcaccagcag
     6721 ttgcgggtgg cgcgcgaagg cgcgcaggct ggccggctgc caggccagca gcgccagcaa
     6781 cgccacgccg aacaccaccg ccgtgctcca cagcgggtgc aggccgtgct ggtgcagggc
     6841 cttgaaggcg atccaggaca gaccccaaac cagcgcgttg cccagcaggg ccagcgccgg
     6901 cagcagggcc gacggcggat gggcggcggc catgggaggc atgccgggga tcaccctgca
     6961 cgcggccggc gcagccgaac gccgctggcg tgatcgacgt cccaggccgg gcgcggcacg
     7021 cgccgagcat gcgggcgcgc cgcctgccca cccctcaggc aaaggcccgc caggccgcaa
     7081 gttcgcctgt gcatacggat ttcaggaaga tgacaagcct caacggccgc tgtgaggttc
     7141 aatgtccgcg atcgcgccgg gcgatgttca gcaggcgttc gaccttgccc gggtagcggc
     7201 gcaggttgtg gcggtgccag cgctggatca gcagcatgaa ggcgatcacc acggcgccga
     7261 acagcgcgat ggcgccgaac gccgacagcc cggcgccggc cgaggcgctg tagagcgcgc
     7321 ccaggcccag gatgcaggcc tgttcgttga agttctgcac cgcgatggag cgcccggagc
     7381 ccatcaggtt gtggccgcgg tgctgcagca gcgcgttcat cggcaccacg atgtagccgc
     7441 ccagcgcgcc catgaagatc aggaacggca cggccagcca cagccggtcg atgaacacca
     7501 tcagcaccac cagcgccccc atcagcacgc ccagcggcat gacgccgatg gcgcggtcca
     7561 gccgcacccg catcgaagcc agcaccgccc cgacggcggt gccgatggcc accaccccca
     7621 ccaggccggc cgcctgggtg gtgctgtagt gcagcgcggc ggcggcccag gccagcacga
     7681 tgtagcgcat gttgcccgac acgccccaga acagcgtggt ggtggccagc gagatctggc
     7741 ccagcttgtc gcgccacagg cgcgcgttgc agcgccagaa gtcgggcagc agcgacagcg
     7801 tgcgcgccgg caacgggcgc atgcgcacgc cggtgtgggg gatgcgcagg ttgatggcgg
     7861 cggcgatggc gtacaccagc accaggctgg cgatcgccgc ctcggccggc gtgtcgatgc
     7921 cgctgtgcag gcccggcaga tccagcccca gcaggcgcgg cgccagctgc gggccgacca
     7981 gctgtccgcc cagcagcacg cccaggatga tggaggcgat cgtcaggccc tcgatccagc
     8041 cgttggcgcg caccagctgc gaaggcggca gcagctcggt caggatgccg tacttggccg
     8101 gcgaatacgc cgccgcgccc agcccgacga tggcgtaggc cagcagcggg tgaccgccga
     8161 acagcatggc caggcagccg acgatcttga tcgcgttgct gaggaacatg acgcgcccct
     8221 tgggacgcga atcggcgaac gcgcccaccc agggcgccag caccacatag aacagcgcga
     8281 acatcggcac cagcgccgcg cgctgccatt cggccgcacc actgccgcgc agcagttcga
     8341 cggcggcgac gaacagggcg ttgtcggcga gcgagctgaa gaactgcgcc gccatgatgg
     8401 tgtagaagcc ccgtctcatg ggcgctgggc cggacacgcc gggccgtggc agatccgaac
     8461 caaagaagta agctcctgta tcggtgcgcg gttatagcac gcgccggcgg tgccagaatc
     8521 gcgctccatc gtccccagcc accctgtcat gccccgcccg atccaagcca ccatccacac
     8581 cggcgcgctg cgccacaacc tcgaacgcct gcgccaggcc gcgcccggtg cccaactgtg
     8641 ggcggtggtc aaggccaacg cctacggcca cggcatcgag cgcgtgttcg aaggcctgcg
     8701 cggcgccgac ggctttgccc tgctcgatct ggccgaggcc gaacgggtgc gcgcgctggg
     8761 ctggcgcggc ccgatcctgc tgctggaagg cgtgttcgag ccgcgcgacc tggagctgtg
     8821 ctcgcgcctg tcgctgtggc acgcggtgca cgacgacgcc cagatcgact ggctggccat
     8881 gcacaagacg caccagccgc accatgtgtt cctgaagctg aactcgggca tgaaccggct
     8941 gggcttcgcg cccgagcgcc tgcgcgccgc ccacgcgcgg ctgtcggcgc tgccgcaggt
     9001 gggcgagatt tcactgatga cccatttcag cgacgccgat ggcgcgcgcg gcatcgccgc
     9061 ccagtgggcg gtgttccagc gcgtcaccga gggcctgccg ggcgagcgca gcgtctgcaa
     9121 cagcgccgcc accctgcgcc acgcgcagga cgcggcggtg cgcgccgact gggtgcgccc
     9181 cggcatcgcc gcctatggcg gctcgcccga ctttccggcg cacgatgcgg cccactgggg
     9241 cctgcagccg gccatgacgc tgcgctcgcg gctgatcgcc accggcagcc tgcaggccgg
     9301 cgacagcatc ggctacggct ccaccttcac cgccgagcgg ccgatgcgcg tcggcgtggt
     9361 cgcctgcggc tacgccgacg gctacccgcg ccacgccccc accggcacgc cggtgctggt
     9421 cgatggcgtg cgcacccgcc tgctggggcg cgtcagcatg gacatgctga gcgtcgatct
     9481 ggagcccgtg ccacaggccg gccccggcag cgcggtgacg ctgtggggcc agggcctgcc
     9541 gatcgacgag gtggcgcacg ccgccggcac catcggctac gagctgatgt gcgcgctggc
     9601 gccgcgcgtg ccggtgcagg tcgactgagc ccgaatggta ttcaagacat cccaaggccg
     9661 catccatcga cgcgatcggc gatcagaaca ggcgccaacg cccatccggc aagcgccagg
     9721 agctctgatt tgaatagcgt tcgtggcacc cgtggcagcg gcgccgccag tcagcgcagg
     9781 aaggcgtcgt agccggtctt gaggatcagc gcccccacca ccacgatgaa caccccgcgc
     9841 acgaagccgg cgccgtgcct gagcgccagc cgggtgccga tcaggctgcc ggtgacgttg
     9901 gcggcggcca tcagcagccc cacctgccac cagacgtggc ccttggcggc gaacagggcg
     9961 atggcggcga cgttggtggc gacgttgagc agcttggcgg cggccgaggc gtgcagaaag
    10021 tcgtagccca gcaggcgcac gaacaggaag atgaagaaac tgccggtgcc ggggccgaag
    10081 aagccgtcgt agccgccgat cacggcgccg atggcgcagg ccagcagggt ctcgcgcccg
    10141 cgctcatggc gcggcgcgtg ggtgcggccc aggtccttct tcgccagcgt gtacagcagc
    10201 accgccagca ggatcagcgg cagggcgcgg cgcaggaagg tggggtcgat caacgtcacc
    10261 gcccaggcgc ccagaaacga acctgccagc gccgccagcg ccgccggccc cagcaccgcc
    10321 cagcgcatct gcacgcgccg ggcgtactgg aaggtggcga tgccggtgcc ccagaccgag
    10381 gcgctcttgt tggtgccgaa cagcgtggcc ggggcggcgt tcgggtagac cgcgaacagc
    10441 gccggcacca gcaccagccc gccgccgccg acgatggcgt cgatgccgcc ggccagcagc
    10501 gaggccagcg agacgaggag caggtccatg aatgcccgaa gaactattga tttgatagct
    10561 tttggcgcag gtctggcaag cgccagaggc caaaaccgtg tgaattatcg tatgggttcg
    10621 gccatgggct gtcttgagct ccagattccg aggccagagg gcatccgtca accgggcaag
    10681 aaaaaagggc gccacatggg cgccctgcaa gtcggtgttg acggaaatga tgatctgttg
    10741 tggtggtctt tgggtttgtc tcctcggccc ttcggcgtgc gcacacgcct gagtgcccgc
    10801 actgtaagcg accgcccggc ggccggccat tggtgcttgc ccgtagggga tttccgccaa
    10861 ggcaggactg gtgagcaatt gctcactttc gtgatgcgct gctgtcgtgg ccgagctggg
    10921 gcagcatcga gccttcgccc agctgcaaca ggccgctgcg gctgtagacc gccagcttgc
    10981 cgcgcgtgtc ggtgatgtcg aggttgcgca tggtcagctg gccgatgcgg tcgagcgggg
    11041 tgaagggcgc gttttcgacc ttttccatgc tcaggcgctc gggcgcgtag gtcaggttgg
    11101 gcgactcggt gttgaggatg ctgtagtcgt tgccgcggcg cagctccagc gtgacttcgc
    11161 cggtgacggc gccggccacc cagcgctggg cggtttcgcg cagcatgatg gcctgcgggt
    11221 cgaaccagcg gccctggtag agcaggcggc ccaggcgcag gccgttgatg cggtactgct
    11281 cgatggtgtc ttcgttgtgg atgccggtga ccaggcgctc gtaggcgatg tgcagcagcg
    11341 ccatgccggg cgcttcgtag atgccgcggc tcttggcttc gatgatgcgg ttttcgatct
    11401 ggtcgcacat gcccaggccg tggcgcccgc cgatgcggtt ggcttccagg aacagctcca
    11461 ccgggtcggc gatctcgcgg ccgttgagcg ccaccggccg cccttcctcg aagcgcacgc
    11521 gcacctcttc ggccttgacc tcgacctcgg gcttccagaa cgccacgccc atgatcgggt
    11581 tgacgatgcg gatgccgctg gacagctctt ccaggtcctt cgcctcgtgg gtcgcgccga
    11641 gcatgttgga atcggtcgaa taggcctttt ctgccgacat cttgtagtca aaaccggcct
    11701 tctgcatgaa ttccgacatt tccttgcggc cgcccagctc gtcgatgaag cgctggtcga
    11761 gccagggctt gtagatcttc agcgccgggt tcgccagcaa cccgtagcgg tagaagcgtt
    11821 cgatgtcatt acccttgtat gtgctgccat cgccccagat gttgacgtca tcttccttca
    11881 tcgcgttgac cagcatggtg ccggtcacgg cccgcccgag cggcgtggtg ttgtagtagg
    11941 tggcgccggc ggtctggatg tggaaggcgc cgcactgcag ggcggcgatg ccttcgttgg
    12001 ccagctgggt gcggcagtcc accagacggg ctttttcggc accgtattcg agcgccttgc
    12061 gcgggatggc gtcgtagtcg gactcgtcgg gctggcccag gttggcggtg taggcgtagg
    12121 gcacggcgcc tttctggcgc atccacagca gggcggccga ggtgtccagg ccgcccgaga
    12181 aggcgatgcc gaccttctgg ccggcgggaa gcgattgcag gatggtgtgg gacatgggct
    12241 ggggcggcgg gcgggcgccc gcgcggcggc tgcgggggtt ggaaaaagcg cgattgtccc
    12301 gcctttttgg cgcgcgccgc cggcggcgcc tgccggcggt ccggtgcgtt gcggccaaat
    12361 cggcctctga cgcacaacca gcgagcgctg gcagctacgc tttcaatagc gtatggcggc
    12421 gccgctcaac cgtccaggcg ctggcgcagg gcgatcacct cttccatcag gtcggccgtc
    12481 agggcggcca gctgcgggtc ggcgtcgtac acctgctcca gctgggcgat gcggcgcgcg
    12541 cggcgcagac tgaggctggc gaagcgccac tcggtgctgg cgccgccgcc cagcggctgc
    12601 agcacgccgg cttgcacatg ggcgtgcacc cattcggggc tcatggcgca ggcgcgcgcc
    12661 agttcgtgca ggctgagcgc ggcgtcgtcc agcagctcga ccagctcggc ggggatggcg
    12721 gtggggcggg aaggcatggc ggtcatcctc tgcgtggccg cggggcgtgc ccgcccatcg
    12781 ggcgcaggcc agccaagcgg cgaaatcgga acatcatgcg ggtctgtaac aggtgacggg
    12841 tgatcgacgg tcgttcagcg agcggtgcgg ccgcgttcag gcgccgcgcg ggtcgaagcc
    12901 ggggtaggcc tcggccagcc gcgcccaggc cgccttctgg gcgtcggtga cggcgccggg
    12961 cacggccagt tcgagctcca gcagcagatc gcccggcgcg ctggccgagg ggatgccgcg
    13021 gcctttcagg cgcagcttgc ggccgctgcc ggagccggcc ggcacgccca cttccaccgt
    13081 cttgccatcg ggcgtggcga cctgcaccgc gccgcccagg gcggcttccc agggcgtcac
    13141 gcgcagccgc tgggtgacgt cgcggccatc gatgcgccag cgcgcatcgg gccggaactg
    13201 cacttccagg aacagatcgc cggccggccc gccgtgccag cccggctcgc cctgaccggc
    13261 cagtcggatc agctggcctg gcttgacgcc ctgcgggatg cgcacctgca gcgtgcgctc
    13321 ctgcggccgc acctggccct gggcgtcgag gtgcgcgccg cgcaggtgga tggcgcgttc
    13381 ggcaccgcgg taggcgtcga tcagttccag ctcgatgcgc gcatggtggt cctggccgcg
    13441 cagcggcgcc tgcgcgccgg cgcccggccg ggcaccgcgg gcaccggcac gggcgcggcc
    13501 gaacagctgc tcgaagaatt cgctgaattc gccgccctcg ccgtgcgggc cgaagccggg
    13561 cgcggcgccg tcggaaaact cgtagccggt gttccagccc ggcggcgggc gcacgtcgtc
    13621 gcccgagcgc gcgccagccg cccaggcctg cgcgcccacg ctgtcgtagg cggcgcgctt
    13681 ttcggggtcg gacagcacgg tgtgggcttc gttgacctcg gccatgcgcc gggcggcgtc
    13741 gggttccttg ctgacgtcgg ggtggtagcg gcgcgccagc ttgcgatagg ccttcttgat
    13801 gtcctccggg ctggcgctgt ggctgacgcc caggatctgg tagtagtcct tgaactgcat
    13861 gcgtttgcct gtgtgtgcca gggcggccac cgaaaccggg ccacccgtcg gcatttatat
    13921 gagggtccgg ccccgggctg caagcggcgc cgggcgggca tcccgcatgc tatgcttaat
    13981 atagctaccg gcgcttgttg cgcctgcgcc ggtgaccgat ttgaaaaccg gctcagtcgg
    14041 agcgcgccgg cgcgcggcgg ttgaccagca gcatgcccag cgccaccagc gccagcgcca
    14101 ccaccaggct gacggtgatc ggctcgccca gccacagcgc gccggccatg agggcgaaga
    14161 tcggcgtcag aaaggaaaac gccgacatcc gcgtggccgg gtaatgcgcc agcagccaca
    14221 tccaggccag gtagctggcg aaggcgccca ccaccgtctg cagcgccagc gagctggcgg
    14281 caaacgcgct ccagcgccag cgccagggct cgcccagcgc cagcgacagc agcggcagca
    14341 ccacggcgct gacggccacc tggtagaaca gcatcttctc gggcgccacg cgcaccagcc
    14401 gggtggcacg gatgctgacc gtggtcagcc cccacagggc accggccgcc agcgccagca
    14461 ggtcgccgcg ccaggtcggc ccggcgccgc cctgcgcaaa gccttcgcgc agcgcgaaca
    14521 gcaccgccgc gaaggcgcag gccaggccca gccactgcag gcggtgcagg cgctcggccg
    14581 gcaccagcca gggcagcacc agcgcgaccc agaacggcga ggtatagaga aacaccgtca
    14641 gccgcgaggc ggtggtgtat tgcaggccca ggtacatggc ggcgaattcg ccggcgaaca
    14701 gcgccccggc cagcaggccc gcgcccaacg tgccgtcgcg ctggaacagc ggcacgccgc
    14761 gccagcggca ccacagccac agcagcgcgg tggcgccgac gaagcgcagc gcggcctgga
    14821 acaccggcgc cagctcgggc agcgtggcct tgaccatcac ctgctgcacg ccccagaaca
    14881 ggcagcagac cagcaggatg gcgatcgccc gcccgtccag gtgctcgcgg cgcgcgctca
    14941 tggcaagccc tgcgcgctga cgcgctgcgg gattcgcgct tgggagcggc ccggcgcgct
    15001 tattcggtgt ccctccgcgc tgacgcgctg cgggattcgc gcttgggagc ggcccggcgc
    15061 gcttattcgg tgtccctccg cgctgacgcg ctgcgggatt cgcgcttggg agcggcccgg
    15121 cgcgctcatg cgccgggccc tgcggcggcg ctcacagcgg atgctccgtg tagaaccggc
    15181 cgccgtgaaa cagcagcggc gcggcgcccg ggcggtgcgc gcaatgctcc acctggccga
    15241 tgaacagcac gtggtcgccc tcgtcgtggc ggctgcggtt gaagcattcg aaatgcgcca
    15301 ccgcgccttc gatcagcggc gccccggcca ccccggggtg ccaggccacg ccggcccagc
    15361 ggtcgcccac cggcgccgca aagcgcagcg ccagctcctg ctgctcggcc gccagcacgt
    15421 tgatggcgta gtgcgagccg cggcgaaacg cgtgcagcga cagcgagcgc aggcccaggc
    15481 tccaggccac caggggcgga tcgagcgaga ccgagttgaa ggaattggcc gtcagcccca
    15541 ccagcgcgcc gtcggcgctg cgcgcgctga cgatggtcac gccggtggcg aacatgccca
    15601 gcgcggcgcg aaattcctgc gcggaaaaag cgggctggtg ggcgcgaagg gcgtggggca
    15661 tgggcgggcg gcgtgggcag ccggccatga tagcgaggcg gccggcgccg cgtgtgcgcc
    15721 ggggtgatcg ccctggcccg gcacccgccg gcggcgtacc atggccccat ggcgctgctg
    15781 ccctccttcg ccaccctggg tcacggcccg ccggtcgtgc tgctgcatgg ttcgggcggc
    15841 ggtgcccgca gcttcgcgcc gcagctcgag tcgctggcca gcctgggctt tcgcgccatc
    15901 gcctgggaca tgccgggcta cggccagagc gcaccgatcg agccctacgg cttcaagggg
    15961 ctggccgagc gtggcgcggc gctgatcgag gcgctggcgc cgctgaccgg cggcgcgccg
    16021 gtggccctgg tcggccacgg cctgggcggc atggtggcgc aggagctggt gctgcgccgc
    16081 cccgatctgg tgcgccagct ggtgctggtg gccagcgtcg ccgcggtgtc ggccgacgac
    16141 ggctacggcc gccacgtggc gcaggcgctg gcctggctgg acgaggggcg cgacatgggc
    16201 tggatcgccg ccgagcgcgt gccccggctg atcggcccgg gggcgctggc cagcggcgtg
    16261 cggctggcca cccactgcca ggccaccgtg cccgccccga cctggcgacg tgccctgctg
    16321 gcgctggccg gcttcgaccg ccgcgccgca ctggcgcgca ttgccgtgcc cacgctgctg
    16381 gtggcgggcg agctcgatcc gctggcgccg ccgccggtgc tgcgcgccat ggcggcgcag
    16441 atcagcggcg cgcacagcga acggctggca cacaccggcc acctgccgca gctggagcgc
    16501 ccgcaggcgt tcgaccgcct gctgctgccc tttctgcgcc acgcgcgggc ctggcggcac
    16561 tgaattttgc tattaagata atagctgtcg gcgcttgccg ggagcgcgcc agagcccgat
    16621 tcaactcagt atcggttcag cgcaccagca gcaccggcac ttcgcagctg gccagcacct
    16681 tggtggccac cgagcccatc accaggccac ccagcgcgcc gtggccgcgc gagcccatga
    16741 cgatgaggtc gaatttgccg ctttcggccg tcttggcgat caactcgccg gcattgccga
    16801 ccttggacag cgtcttcacg ttcagctggt ggcgggtcag gaacttgacc accgggtcca
    16861 gcaccttggc cgcttcttcg gcgtagtacg tctccacgat ctcgcgtccc accgccgcgc
    16921 gcgcccgcgg cggcagcgcg ccgtgcaccg tcagcgcggt gtattcgatg tccccggaaa
    16981 acaggtccag gtgcgtggtc aggtaggcga gcatcttctt ggtgaaggcg ctgccgtcga
    17041 cggcaagcag aatcttcatc gcgcggtcct cgaaattgcg ggttgggggg cggacagtgt
    17101 aagcgccccc tggcgggggc cgtccatgac ggcgctcaag cctcgcgctg gcgcgccggc
    17161 gccacgctca cccaggcgtc ggggacgaag ccgtgccgct cgcccttgct cgcgcagggc
    17221 ctgctgacgc tatgcaaggg gaccgcgttg cttcgccaag ggaagtgctg ctcgccccgc
    17281 cccggcagcg ccgagccgcg ctggcaggca ccgacgtcgc ccgccagcac cagcaggtcg
    17341 gccaccggcg ccggccgcgg tacgacgtcg ggctggcgct ccgggtgcag gtcgggccgc
    17401 agttggatgt tcacgacgga cggcgcagtc gatggcgcgc cgccggtgtg acacgaaccc
    17461 cttcccgcca tggggtcttg gtgtaaaccc ttaggggtta accctaggtg cgactacaat
    17521 ttctccatcg gccaaaagcc ttgctggaga acgcccgacg tgacccgaac ggcgtgtgat
    17581 gaccatgtgg aatgtctgga tccgccatct gacgaccctg ttcgccccgc tcaccagggc
    17641 gccccggcac ggcccggggg tgcccgccag cttgctggag cgcgccgacg cctgtgccgg
    17701 gcgtgacccc cggggcgccg ccgacctgcg cgccgcggcc ctgaactatc tgggtgtggt
    17761 gcgctgagcc tcacccgtca tcgcgaacca aggaacccac catgagcacc accagcctga
    17821 ccctcggccg cacggccgtc cgtgaaccgc agttcgaacc cgcccgcctg gaccactggc
    17881 tgccgcgccc gcagaccggc ggcccgagca tgagccagct gctgcgcgca cgctggaacc
    17941 agctgcgcca gcgcctggcg caggcgcgca gcgaacgcga aatccaggcc ctggccgacc
    18001 tcgaccaccg cgtgcagcgc gacctgcaga cggcgcgcga cctggccgaa tggaaagtgc
    18061 cgcaccgccc gttcgtctga accgcaccgg caaggtggct gcgccgcctg ggccgaccaa
    18121 cgaaaagccg cccgcgggcg gcttttttgc gtgccggtgc gacacggcgt caagccatca
    18181 cgcctgctga cccctgaccc aagggtgagg ggtcgggcgg ggcgccgtca ggccgcctcc
    18241 agccgctgcg ccagcgcctg tcgggtggcc agcagctcgg gcggcaggcc ctgggccagc
    18301 ttggtgaaca gctcgtcgtg cagggcgaat tcctgtgccc agacgtcggc gtcgatgcgg
    18361 gtgacggtgt cgaactgggc ggggctgaag ctcaggccgc tccagttgat ctcgtcatgg
    18421 cgcggggcga cgccaaacat ggtgggctga ccggcggcgc ggccttcgac gcggtcgatc
    18481 atccacttga gcacgcgcat gttctcgccg tagccgggcc agacgaactt gccggcggcg
    18541 tccttgcgga accagttgac gcagtagatg gcgggcaggc gcgcgccggc ggcctgcagc
    18601 cgctggccca gcttcagcca gtggccgaag tagtcggcca tgttgtagcc gcagaagggc
    18661 agcatggcga acgggtcgcg ccgcaccacg ccctgggcgc caaaggcggc ggcggtggtc
    18721 tcggagccca tggtggcggc catgtacacg ccctcctgcc agtcgcgcgc ctcggtcacc
    18781 agcggcacgg tggtggagcg gcggccgccg aagatgaagg cgtcgat
//
LOCUS       DAOOOG010000032        18199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_3165_length_18199_cov_26.179894, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000032 DAOOOG010000000
VERSION     DAOOOG010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 18199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18199
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3165_length_18199_cov_26.179894"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PK242_04280"
     CDS             complement(<1..481)
                     /locus_tag="PK242_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOZ93266.1"
                     /translation="MSTLTIGLLLFVAMLLLMAIRVPIAVAMFVPGAVGYVILSGWDP
                     LLAHLRGAVYGRVSVYDLSVIPLFMLMGGFAVHGGLSKALFDFANALLGRFKGGMAMA
                     GILACAAFGAISGSTVATTATIAQVAYPEMRRISYSGRLATAALATGGTMGVLLPPSV
                     "
     gene            complement(478..1005)
                     /locus_tag="PK242_04285"
     CDS             complement(478..1005)
                     /locus_tag="PK242_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOZ93267.1"
                     /translation="MTADNRMREGPVGHLLHVLCDICAVVGGAVLIAMAIMTVVSVIG
                     RTFFDSPILGDVELVQLGLAVCVATFLPYTQFRSANIIVDFFTTRASARSQRWMDGLG
                     CLLYAGMTALIAWRVYAGGVMSQENQEASMLMNLPLWIPYMLMVPGLVLCTLVGLYQA
                     VIYWTQPAAAEGDAA"
     gene            1526..1939
                     /gene="gloA"
                     /locus_tag="PK242_04290"
     CDS             1526..1939
                     /gene="gloA"
                     /locus_tag="PK242_04290"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HOZ93268.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTQVLGMQLLRTSENPEYRYSLAFV
                     GYEGGNPGQAEIELTYNWGTDSYDLGTAYGHIAIGVPDAYAACERIRAAGGQVTREAG
                     PVKGGTTVIAFVTDPDGYKIELIQRAEGAAGGGLR"
     gene            complement(2043..3104)
                     /gene="tolA"
                     /locus_tag="PK242_04295"
     CDS             complement(2043..3104)
                     /gene="tolA"
                     /locus_tag="PK242_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HOZ93269.1"
                     /translation="MNVAAIERAEFAPPPQGGFGRAFGLALAAHLLLILALTWGLRWK
                     NEPQDLAVQAELWSPTVQRAAPKAAPRAPAPQVEPPPPPAAPPQPRPEPALKPPPPQV
                     DEQAQRDADIALARKKQEEARQAAAQREQARREREKKEQAEQERRELAQKKAAEEQKR
                     KQDELRKLAAKKQAEEAEQRRQAAAEAKKKAEAQKRAEAEAERKAEAQAKARAAAQAR
                     AAEDARHKANVARSLAMAGSADSEGGSAAGKGRGGGSAGGQDERDAGPSGNYAGRIRA
                     AVRPNIVFTDDAPGNPVAEVEVRTDQVGNIISRRITRSSGVRTWDDAVLRALDRTEKL
                     PSDNGRYWNPMLITFRLRD"
     gene            complement(3115..3486)
                     /locus_tag="PK242_04300"
     CDS             complement(3115..3486)
                     /locus_tag="PK242_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HOZ93270.1"
                     /translation="MNEINMVPFIDVMLVLLIIFMVTAPLITPGVINVPSAGQSSKAP
                     DQRVDVQIDAEGKLSLAGQIQRSGLGDKEAVAAIRALLADNPELPVMIAADSELRYQQ
                     VMNMMKLLREAGVQRVGLAVK"
     gene            complement(3530..4243)
                     /gene="tolQ"
                     /locus_tag="PK242_04305"
     CDS             complement(3530..4243)
                     /gene="tolQ"
                     /locus_tag="PK242_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOZ93271.1"
                     /translation="MNQDLSIIQLVLHASFVVQLVMLLLLVVSITSWAAIIRKLRSFK
                     QTRALNDEFERDFWSGTSLNDLYASAVQNAKHAGPMERIFASGMREYQKLRERRIGDP
                     SALLDGARRAMRASQQRELDEAESNLSFLASVASVSPYVGLFGTVWGIMHAFTGLASL
                     QQVTLATVAPGIAEALVATAIGLFAAIPAVVAYNRFARELDRLAIKLETFIEEFSNIL
                     QRNLGVHAPAAHSATPTGY"
     gene            complement(4240..4656)
                     /gene="ybgC"
                     /locus_tag="PK242_04310"
     CDS             complement(4240..4656)
                     /gene="ybgC"
                     /locus_tag="PK242_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HOZ93272.1"
                     /translation="MAEFEFPIRVYWEDTDAGGIVFYANYLKFFERARTEWLRAGGVH
                     QRSLREQVGGMFVVVESQVKYLRPARLDDELVVTARLQEAGRAGLVIDQQAFVRRQAP
                     EPRELLCRARIRVGWVDAVTLKPARIPPDILKTLPT"
     gene            complement(4668..5885)
                     /locus_tag="PK242_04315"
     CDS             complement(4668..5885)
                     /locus_tag="PK242_04315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOZ93273.1"
                     /translation="MRISARAQRIEPFYVMEMAKSAAAIAREAAHGDRPMIYLNIGEP
                     DFTAPPLVQAAAERAIRDGHTQYTQATGLPLLRERISQWYADRFGLRVPARRIVVTAG
                     ASAALQLACLALIEAGDEILMPDPSYPCNRQFVSMAEGRAVLLPSTAAERFQLSADKV
                     RQAWGAHTRGVMLASPSNPTGTSIHPDELARIHAVVSERGGITLIDEIYLGLSYDDTY
                     GHSALALDDQIISINSFSKYFNMTGWRLGWLVVPDALVEVVEQIAQHLFICASTVSQH
                     AALACFEPDSLAEYERRRAEFRARRDWFVPQLGALGLTVPVMPDGAFYAWADCSAACD
                     RMFAGRSEYNSEMQHPGSWEFAFELMKRAHLAVTPGRDFGHAETARCVRFSTANSMAQ
                     LHTAIERLKAVLA"
     gene            complement(5882..6424)
                     /gene="nusB"
                     /locus_tag="PK242_04320"
     CDS             complement(5882..6424)
                     /gene="nusB"
                     /locus_tag="PK242_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOZ93274.1"
                     /translation="MSPAPTTPRAAGAAPRPGEGGTRKSSVKSPRSRAREFALQGLYQ
                     HLVGGGDAAAIDVFTRELQGFAKADAAHFDALLHGCIELADDLDALIVPLLDRRLADI
                     SPIERGCMWIGAYEFLKCPDVPWRVVLNECIELAKDFGGTDGHKYVNAVLNGLAPQLR
                     APEVAADRARVRAAKGRDAT"
     gene            complement(6421..6885)
                     /gene="ribH"
                     /locus_tag="PK242_04325"
     CDS             complement(6421..6885)
                     /gene="ribH"
                     /locus_tag="PK242_04325"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOZ93275.1"
                     /translation="MLGADKGQTLSLDGSALRIGIVQARFNEGVTQALADACLGELAA
                     LGVPAAQIVHHTVPGALEVPLALQWLARRGECHALIALGCIIRGETYHFELVANESGA
                     GVTRVALDLDIPVANAILTTENLAQAQARQTDKGRDAARVAVEMALLKAGLR"
     gene            complement(6897..8033)
                     /gene="ribBA"
                     /locus_tag="PK242_04330"
     CDS             complement(6897..8033)
                     /gene="ribBA"
                     /locus_tag="PK242_04330"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOZ93276.1"
                     /translation="MNAPLSSPAAASTISPVPDIVAELAAGRMVILVDEEDRENEGDL
                     ILAADHVTPEAINFMARFGRGLICLTLTRERCERLRLPPMVTRNGTKMGTAFTVSIEA
                     AEGVTTGISAADRARTVQAAVAAQARADDLVQPGHIFPLQAVDGGVLMRAGHTEAGCD
                     LAAMAGCTPAAVICEIMKDDGTMARLPDLQLFAAEHGLKIGTIADLIEYRSRTESLIE
                     KIASRPLRTAHGEFIAHAYRDQPSGALHLGLVLGQWQPGDTVPVRVHEPLSVLDALEV
                     QRGSHSWSVHESLQHIQSAGRGVAVFLNCGEDAGQLLAQFQGTARAAHAPERGRMDLR
                     SYGVGAQILRDLGVQRMQLLGTPRRLPSMAGGYGLEVTGYITKD"
     gene            complement(8307..9572)
                     /locus_tag="PK242_04335"
     CDS             complement(8307..9572)
                     /locus_tag="PK242_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOZ93277.1"
                     /translation="MEPVRLMREMFEAAIASAQPARCVPAHLPNPAEVRGRLIVVGAG
                     KASAAMARAVEEHWRGPLDGLVVTRYGYHVPCQHIRIVEAAHPVPDAAGLQAAERMLG
                     LVSGLTPDDVVLCLISGGGSSLLPLPLPGITLADKQQVNRALLASGASIGEMNTVRRH
                     LSAIKGGRLAAACHPARVITLVLSDVPGDVPMDIASGPTVGDPTTCADALDIVQRYGI
                     ELPAPVRVLLDSGRGESVKPDDARLAGHTTRMVAAPQQALEVAAQVARRHGYAAHILS
                     DAVEGEAREVGKVLAALALQVAGRGQPFTAPCAILSGGETTVTLRGQGRGGRNVECLL
                     SLAIATRGHARIHALMGDTDGVDGAQEVAGAHIGPDTLARAFAQGIHPRRSLDDNDAH
                     GFFQALGDAVVTGPTLTNVNDFRVILVNA"
     gene            complement(9964..10599)
                     /gene="pdxH"
                     /locus_tag="PK242_04340"
     CDS             complement(9964..10599)
                     /gene="pdxH"
                     /locus_tag="PK242_04340"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOZ93278.1"
                     /translation="MKDIAHLRKSYERAELSEDASHADPGRQFAQWFEEARQSELPEP
                     NAMTLATVGSDLRPSTRVVLIKEFDERGIVWYTNYESRKGRQLAGNPYAALQFHWVEL
                     ERVVRIEGLVEQVSEAESDAYYASRPLDHRLGAWASPQSQVIPGRAWLAAEAAKVGAR
                     FLLNPPRPPHWGGFRLRPDRWEFWQGRKSRLHDRLCYRLEAGGWVRERLAP"
     gene            complement(10765..11415)
                     /locus_tag="PK242_04345"
     CDS             complement(10765..11415)
                     /locus_tag="PK242_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOZ93279.1"
                     /translation="MRSADAPLPPAYADFAHLRAPGRDPLRLLEHTRAQWHRRAGPQA
                     DLWVFGYASLIWRPDFDFSERHATRVHGWHRALAMWSRVNRGTPEQPGLVFALLPGGS
                     CQGMVFRVPRGTADAVFDTLWAREMPSGVYDARWLPCATPHGIVWALAFTLSRRSPSF
                     TGALTPEQYRHIFRHARGRYGTTLDYARRTLETLGAHGIHDRRLRTLLSLVDDEEA"
     gene            11460..12023
                     /locus_tag="PK242_04350"
     CDS             11460..12023
                     /locus_tag="PK242_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ93280.1"
                     /translation="MSEQREEERGVVWAVLIGVVLLAIGLAVGMGLYRTGKGVAPAAA
                     SSAANANANAGGTAAGGAAGAPANTAAMTSGEVGDGAAIRVENGVVKFYFASGSAELA
                     AGAAEALGEVVKGVAAGKKAVISGFHDVTGDPVRNEELAKQRALAVRDALAALGIGED
                     KIDLRKPAVTTASGSNAEARRVEVHLE"
     gene            complement(12352..15849)
                     /gene="dnaE"
                     /locus_tag="PK242_04355"
     CDS             complement(12352..15849)
                     /gene="dnaE"
                     /locus_tag="PK242_04355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOZ93281.1"
                     /translation="MFVHLRLHTEFSVVDGTTRLDDIVQAAADDAQPALAITDLGNLF
                     GAIKFYKAARGAGVKPIIGAEVLLEGQGSDPEAPTRVLLLVTSGQGYLNLSELLARAW
                     TQGVVKGQAVVRWAWLGELADGLILLSGAQAGPVGQALAQGGVEQARALALQLAELFP
                     HRFYLELQRAGRADDEAHVAAAVQLAARLKLPVVATHPVQFARADDYEAHEARVCIAE
                     GEILGNPRRVRRFTPEQYFKSTAEMQQLFADLPAALSNAAEIARRCNLTLALGKPRLP
                     DFPVPQGHTVETYFRQTSTDGLEQRLAHLYPDPSQRDAERPRYLQRLEFEIDTILKMG
                     FPGYFLIVGDFINWAKNHGCPVGPGRGSGAGSLVAYALKITDLDPLQYKLLFERFLNP
                     ERVSMPDFDIDFCQANRDRVIDYVKDKYGRDAVSQIATFGTMAARAAIRDVGRVLDMS
                     YTFCDGISKLIPNKPGQHITIDGAMKVEPLLAERYRKEDEVRTLIELAQKLEGMTRNV
                     GMHAGGVLIAPGKLTDFCPLYQQPGSEAAVSQYDKDDVEAVGLVKFDFLGLATLTILE
                     IAKELIVERHPGQQHFAYENLPLDDAPTYHLFAQGRTEAVFQFESRGMQGMLRDARPT
                     RLEDLIALNALYRPGPMDLIPSFVARKHGREEVVYPHPLVADMLSETYGIMVYQEQVM
                     QTAQILGGYSLGGADLLRRAMGKKKPEEMAQHRQIFREGAARNGISEGKADEIFDLME
                     KFAGYGFNKSHAAAYSLLAYHTAWLKVHYTAEFYCANMTVEMDNTDKLKVLYEDAQRE
                     GMRFESPDINRGGYRFEPVSNTLIRYGLGAIKGTGQQAIEAIVAAREGRGECPAGAER
                     GPFTSLFDFCRRVDRTRVNKRTVEALIRAGAFDALQLNRAALAASIDLAFDFAAASEA
                     NVHQGGLFDLMEDGHGSSSQEPELPAVTPWGVKERLSLEKTAIGFYLSGHLFDEVERE
                     VRQFARRSIAELADSREPQLLAGIVGDLRIINGQRGKLALFRLDDKSGVIEARAEEAL
                     IHRHRHILKDDEFIVVMGKAMPDRFSGGLQLNVTQIWDLAQARCRFGKYLRVAVAGKA
                     PEVRRLLAEYPPQVEHTEHGELLRGLPVRLLLERRGSEGGVMAQLHLGEQAQFFPSDA
                     ALASWTAQADEGRAMVVYE"
     gene            15997..17064
                     /locus_tag="PK242_04360"
     CDS             15997..17064
                     /locus_tag="PK242_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ93282.1"
                     /translation="MTPPRPHLSHRIAVVGAGIAGLACARTLRQAGHEVAVFEAAPQP
                     GGRMATVQTAFGGFDAGVQYFTVRDERFMQALQATASDVVRRWSANAVRVLDERGRVV
                     EAAPPAPESHWVAVPAMDRLPQRWAEPLVAAGALHLDTRVWRVAHDRLSPGRWQLHTD
                     GPGEDHPVQAGFDQVLLAIPPQQANELLQASTLSTAPFAALGAVDVAPCWTLMLAYPN
                     ATQPGLHTLGPQWNAARSTHHRVSWLARESSKPGRVAVERWTVQASAAWSREHERDDP
                     ARVSAKLLRAFAEITGIHATPAHTEALLWRQARTLAPLGRTHLWDGAGSIGLCGDWCL
                     GCRVEDAFVSGLELALAVIAA"
     gene            17105..18058
                     /gene="gluQRS"
                     /locus_tag="PK242_04365"
     CDS             17105..18058
                     /gene="gluQRS"
                     /locus_tag="PK242_04365"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HOZ93283.1"
                     /translation="MYVGRFAPSPTGPLHAGSLVAALASWLDARAHDGRWLVRIEDVD
                     TPRCVPGADRQILQQLAACGLPPDQPPVWQSGRSAHYQAALDALVTAGAAYPCGCSRK
                     DIEQAQAARGLRRQRGVELPYPGSCRPERGGLQGKPARAWRLHTGVHESNPPPGQSGQ
                     APAAIVSEATNEAIVWHDRRLGPQRQDVAHEVGDFVLRRADGLWAYQLAVVVDDAAQG
                     VTHVVRGADLADNTPRQILLQRRLGLATPRYLHTPLVLAADGEKLSKQHGAQALDVSD
                     PLRTLQRAAAVLALDPKAGTSVPEALAAWTAQWRQRWVQAD"
BASE COUNT         2781 a         6352 c         6263 g         2803 t
ORIGIN      
        1 tcaccgaagg cggcagcagc acgcccatgg tgccgccggt ggccagcgcc gccgtcgcca
       61 ggcgccccga atagctgatg cgacgcattt cgggataggc cacctgggcg atggtggcgg
      121 tggtcgccac ggtggagccg gaaatggcgc cgaaggccgc gcaggccagg atgccggcca
      181 tcgccatgcc gcccttgaag cgccccagca gcgcgttggc aaagtcgaac agcgccttgg
      241 acaagccgcc atgcacggca aaaccgccca tcagcatgaa cagcgggatc accgacaggt
      301 cgtacaccga cacccggccg tacaccgccc cgcgcaggtg cgccagcagc gggtcccagc
      361 ccgacaggat cacgtaaccc accgcgccgg gcacgaacat ggcgacggca atcggcaccc
      421 ggatcgccat cagcagcagc atggcgacga acagcagcaa accgatggtc agggtgctca
      481 tgcggcgtct ccctcggccg cggcgggctg cgtccagtag atcaccgcct ggtacaggcc
      541 gaccagggtg cacagcacca ggccgggcac catcagcatg tagggaatcc agagcggcag
      601 gttcatcagc atcgaggctt cctggttctc ctggctcatc acgccaccgg catacacccg
      661 ccaggcgatc agcgccgtca tgccggcata cagcaggcag cccaggccgt ccatccagcg
      721 ctggctgcgc gccgaggccc tggtggtgaa gaagtcgacg atgatgttgg cgctgcgaaa
      781 ctgcgtgtac ggcaggaagg tggccacgca caccgccagg ccgagctgaa ccagctccac
      841 atcacccagg atggggctgt cgaagaaggt acggccgatc acgctcacca ccgtcatgat
      901 ggccatggcg atcagcaccg ccccgccgac cacggcgcag atgtcgcaca gcacgtgcag
      961 caggtgcccc accggcccct cgcgcattcg attgtctgcc gtcatcccgg ctcatctcct
     1021 cttgagtatc cgtggccgta cccgacagcc gcaagcgcac gccgccacag gacagcgttg
     1081 acgggcggca agtctaacca gtctccaccc gtccacacac aggccaatcc ctacgcgggg
     1141 ccgcttattc tatggcaaat agattcgctc atgtttaacc tggaaacggc agttggccgc
     1201 ttgtcatctt cgggcagacc gcatcgatgc ggggtctggc ggctgcgatg gcctgcgcct
     1261 cctgggtgag cacgctgcgc acccacccgc accgcgctcg gcgcgccatg cggcgttgct
     1321 cctccttgcg cgcaggagcc cgcggcgtcg tcgcgtcttg catggcacac cgatcacggc
     1381 ccgggcgggc gcgtccaacg gccgtgtcta ggtttcatcg tgcgggttgt cgtgtattga
     1441 aggtgcaagc tcggttcgac acatcccgtg gcggtggcgg gcgcccggcc agcggcgatc
     1501 cggccgccga cggcacaatg gcggcatgag acttctgcac accatgcttc gtgtcggcga
     1561 tctgcaacgt tcgatcgact tctacaccca ggtgctcggc atgcagctgc tgcgcacctc
     1621 ggagaacccg gagtaccgct actcgctggc gttcgtcggc tacgaaggcg gcaacccggg
     1681 acaggcggag atcgagctga cctacaactg gggcaccgac agctacgacc tgggcaccgc
     1741 ctacggccac atcgccatcg gcgtgcccga tgcctacgcc gcctgcgagc gcatccgtgc
     1801 cgccggcggc caggtcacgc gcgaggccgg gccggtcaag ggcggcacca cggtgatcgc
     1861 cttcgtcacc gatccggacg gctacaagat cgagttgatc cagcgcgccg aaggcgccgc
     1921 cggcggcggg ctgcgctgag gcgccgccgc agggttcaag gtttttttgc ctctggcgct
     1981 tgctggtatt gcgccgacag ctatgttttt aatagcgaac tgaagcgccg ccctgctccg
     2041 gctcagtcac gcagcctgaa ggtgatcagc atcgggttcc agtagcgccc gttgtccgag
     2101 ggcagcttct cggtgcggtc gagcgcgcgc agcaccgcgt cgtcccaggt cctgacgccg
     2161 ctggagcgcg tgatgcggcg gctgatgatg ttgccgacct ggtcggtgcg cacctcgacc
     2221 tcggccaccg ggttgcccgg cgcatcgtcg gtgaacacga tgttgggtcg caccgcggcg
     2281 cggatgcgac ccgcgtagtt gcccgaggga ccggcgtcgc gctcatcctg acccccggcg
     2341 ctgccgccgc cccggccctt gccggcggcg ctgccgcctt cgctgtcggc gctgccggcc
     2401 atggccagcg agcgcgccac gttggccttg tgccgtgcgt cctcggccgc cctggcctgg
     2461 gccgccgccc tggccttggc ctgcgcttcg gccttgcgtt cggcctccgc ctcggcgcgc
     2521 ttttgcgcct cggccttctt ctttgcctcg gcagcggcct gccggcgctg ttcggcttct
     2581 tcggcctgtt tcttcgcggc cagcttgcgc agctcgtcct gcttgcgctt ttgctcttcg
     2641 gcggccttct tctgggccag ctcacggcgc tcctgctcgg cttgctcctt tttctcgcgt
     2701 tcgcgccttg cctgttcgcg ctgggcggcg gcctggcggg cctcttcctg cttcttgcgc
     2761 gccagcgcga tgtcggcgtc gcgctgcgcc tgctcgtcca cctgaggggg cggcggcttg
     2821 agcgccggct cgggcctggg ctgcggcggc gccgcaggag gtggcggcgg ctcgacctgc
     2881 ggcgcaggcg cgcgcggcgc cgccttgggc gcagcccgct ggaccgtggg cgaccacagc
     2941 tcggcctgca ccgccagatc ctgcggttcg ttcttccagc gcaggcccca ggtgagtgcc
     3001 aggatcagca gcaggtgcgc cgccagcgcc agcccgaagg cgcgtccgaa gccgccctgc
     3061 ggcggcggcg cgaactcggc gcgttcgatg gcggcgacgt tcatggcgcg gccctcactt
     3121 gacggccaga ccgacgcgct gcacaccggc ttcgcgcagc agcttcatca tgttcatcac
     3181 ctgctggtag cgcagctcgc tgtcggcagc gatcatcacc ggcaactccg ggttgtcggc
     3241 cagcagcgcc cggatggcgg ccaccgcctc cttgtctccc aggccgctgc gctgaatctg
     3301 gccggccagc gagagcttgc cttcggcgtc gatctgcacg tccacccgtt ggtcgggcgc
     3361 cttgctcgac tggccggcgc tgggcacgtt gatgacgccg ggcgtgatca gcggcgccgt
     3421 caccatgaag atgatcagca gcaccagcat cacgtcgatg aacggcacca tgttgatctc
     3481 gttcatggcg cggcggcctc ggccgcggga gctgacggcg ggcacggctt cagtatccgg
     3541 tgggggtggc cgaatgcgcc gccggagcat gcacgcccag gttgcgctgc aggatgttgg
     3601 agaactcctc gatgaaggtc tccagcttga tcgccaggcg atcgagctcg cgcgcgaagc
     3661 ggttgtaggc caccaccgcc gggatggcgg cgaacaggcc gatggcggtg gccaccaggg
     3721 cctcggcaat gcccggcgcc acggtggcca gcgtcacctg ctgcaggctg gccaggccgg
     3781 tgaaggcgtg catgatgccc cacacggtgc cgaacaggcc gacgtaggga ctgaccgagg
     3841 ccaccgaggc caggaacgac aggttggatt cggcctcgtc cagttcgcgc tgctggctgg
     3901 cgcgcatggc gcggcgcgcc ccgtcgagca gcgcgctggg atcgccgatg cggcgctcgc
     3961 gcagcttctg gtactcgcgc atgccgctgg cgaagatgcg ctccatcggc ccggcgtgtt
     4021 tggcgttctg caccgccgag gcgtacaggt cgttcagact ggtgccggac cagaagtcgc
     4081 gctcgaactc gtcgttgagc gcccgcgtct gcttgaacga gcgcagcttg cggatgatcg
     4141 cggcccagct ggtgatggac accaccagca gcagcagcat caccagctgc accacgaagc
     4201 tggcgtgcag caccagttga atgatggaaa ggtcctggtt catgtaggca gcgttttcag
     4261 aatgtcgggc ggaatgcgag cgggtttcag ggtgacggca tcgacccagc cgacacggat
     4321 gcgggcacgg cacagcagct cgcgtggctc gggcgcctgg cgtcgcacga aggcctgctg
     4381 atcgatcacc aggcccgccc gaccggcctc ttggagccgc gcggtgacca caagttcatc
     4441 gtccagccgc gccgggcgca ggtatttcac ctgcgactcg accaccacga acatgccgcc
     4501 cacctgctcg cgcaggctgc gctggtgaac gcccccggca cgcagccatt cggtgcgtgc
     4561 gcgctcgaag aacttcaggt agttggcgta gaacacgatg ccgccggcat cggtgtcttc
     4621 ccagtagacg cggatcggaa actcgaattc cgccatgcac caccgcctca ggccagcacc
     4681 gccttcagcc gctcgatggc ggtgtgcagc tgcgccatcg agttggcggt ggaaaagcgc
     4741 acgcagcgcg cggtctcggc atgcccgaag tcgcgccccg gcgtgaccgc caggtgcgcg
     4801 cgcttcatca attcaaaggc gaattcccaa ctgcccggat gctgcatttc gctattgtat
     4861 tcggagcggc cggcgaacat cctgtcacag gcggcggagc aatcggccca ggcgtagaag
     4921 gcgccgtcgg gcatcaccgg caccgtcagg cccagggcgc ccagctgcgg cacgaaccag
     4981 tcgcgccgcg ccctgaattc ggcgcgccgg cgctcgtact cggccaggct gtcgggctcg
     5041 aagcaggcca gcgccgcgtg ctgcgacacg gtgctggcgc agatgaacag gtgctgggcg
     5101 atctgctcga ccacttcgac cagcgcatcg ggcaccacca gccagcccag gcgccagccg
     5161 gtcatgttga agtacttgct gaagctgttg atgctgatga tctggtcgtc cagcgccagc
     5221 gcgctgtggc cgtaggtgtc gtcgtaggac agaccgaggt agatctcgtc gatcagggtg
     5281 atgccaccgc gctcgctcac caccgcgtgg atgcgcgcca gctcgtcggg gtgaatcgag
     5341 gtgccggtgg ggttggaggg cgacgccagc atgacgccgc gcgtgtgtgc gccccaggcc
     5401 tggcgcacct tgtcggcact gagctgaaag cgctcggccg cggtggacgg cagcagcacc
     5461 gcacggccct cggccatgct gacgaactgg cggttgcagg ggtagctggg atcgggcatc
     5521 aggatctcgt cgcccgcctc gatcagcgcc aggcaggcca gctgcagcgc cgccgaggcg
     5581 ccggcggtga cgacgatgcg acgcgccggc acccgcagcc cgaagcggtc ggcgtaccac
     5641 tggctgatgc gctcgcgcag cagcggcagc ccggtggcct gggtgtactg ggtgtgaccg
     5701 tcgcggatgg cccgctcggc cgccgcctgc accaggggcg gcgcggtgaa atcgggctcg
     5761 ccgatgttca ggtagatcat cggccgatcg ccgtgcgcgg cttcgcgggc gatggcggcg
     5821 gccgacttgg ccatctccat cacgtagaac ggctcgatgc gctgggcgcg ggcagagatc
     5881 ctcatgtcgc gtcccggccc ttggcggcgc gcacccgggc gcggtcggcg gccacctcgg
     5941 gcgcgcgcag ctgcggcgcc aggccgttga gcacggcgtt gacgtatttg tggccgtcgg
     6001 tgccgccgaa gtccttggcc agttcgatgc actcgttgag caccacgcgc cagggcacgt
     6061 cggggcactt gaggaattcg taggcaccga tccacatgca gccgcgctcg atgggcgaga
     6121 tgtcggccag ccggcggtcc agcagcggca cgatcagcgc gtcgaggtcg tcggccagct
     6181 cgatgcagcc gtgcaacagg gcgtcgaaat gcgccgcatc ggccttggca aagccttgca
     6241 gctcgcgcgt gaagacgtcg atcgcggccg cgtcgccacc gccgaccagg tgctggtaca
     6301 ggccctgcag ggcaaattcg cgcgcgcgcg aacgcggcga tttgaccgaa gacttgcgcg
     6361 tgccgccttc gccggggcgc ggcgcggcgc cggccgcgcg cggcgtggtg ggtgccgggc
     6421 tcatcgcagg cccgccttca gcagcgccat ttcgaccgcc acccgggccg catcgcgccc
     6481 tttgtcggtc tgccgcgcct gggcctgggc caggttctcg gtggtcagga tggcgttggc
     6541 caccgggatg tcgaggtcca gcgccacgcg cgtgacgccg gccccggact cgttggcgac
     6601 cagttcgaaa tggtaggtct cgccgcgaat gatgcagccc agcgcgatca gcgcatggca
     6661 ttcgccgcgc cgcgccagcc actgcaaggc cagcggcact tcgagcgcac ccggcacggt
     6721 gtggtggacg atctgggccg ccggcacacc caatgcggcc agctcgccca ggcaggcgtc
     6781 ggccagggcc tgggtcacgc cttcgttgaa gcgcgcctgc acgatgccga tgcgcagcgc
     6841 gctgccgtcg agtgaaagag tctgtccttt gtcggcacca agcatgggcg tccttctcaa
     6901 tccttcgtga tgtagccggt cacttccagg ccgtagccgc ccgccatgct gggcaggcgc
     6961 cgcggcgtgc ccagcagctg catgcgctgc acgcccaggt cgcgcaggat ctgcgcgccc
     7021 acgccatagc tgcgcaggtc catgcgcccg cgctcagggg cgtgggcggc gcgcgccgtg
     7081 ccctggaact gcgccagcag ctgaccggcg tcttcgccgc agttgaggaa cacggccacg
     7141 ccgcgcccgg cgctctggat gtgctgcagg ctttcgtgca cgctccagga atggctgccg
     7201 cgctgcacct ccagcgcatc cagcaccgac agcggctcgt gcacgcgcac cggcacggta
     7261 tcgccgggct gccactgccc cagcaccaga cccagatgca gcgcgccgct gggctggtct
     7321 cggtaggcgt gggcgatgaa ttcgccatgt gcggtgcgca gcgggcgcga ggcgattttt
     7381 tcgatcagcg attcggtgcg gctgcggtac tcgatcaaat cggcgatggt gccgatcttc
     7441 agcccgtgct cggcggcaaa cagctgcagg tcgggcaggc gcgccatggt gccgtcgtcc
     7501 ttcatgatct cgcagatcac cgccgccggc gtacagccgg ccatggcggc cagatcgcag
     7561 ccggcctcgg tgtggccggc gcgcatcagc acgccgccgt cgacggcctg cagcgggaag
     7621 atgtggccgg gctgcaccag gtcgtcggcg cgggcctggg ccgccaccgc ggcctgcacg
     7681 gtgcgcgccc ggtcggcggc gctgatgccg gtggtgacgc cttcggcggc ttcgatggag
     7741 accgtgaagg cggtgcccat cttggtgccg ttgcgcgtca ccatgggcgg caggcgcagg
     7801 cgctcgcaac gctcgcgcgt gagggtcagg cagatcaggc cgcgaccgaa gcgcgccatg
     7861 aagttgatcg cctcgggcgt gacatggtcg gccgccagga tcaggtcgcc ttcgttttcg
     7921 cgatcctctt cgtcgaccag aatcaccatg cggccggcgg caagctcggc cacgatgtcg
     7981 ggcaccggcg agatggtcga agcagcagcg ggggaagaca ggggagcgtt catggcggtc
     8041 acgttcggtg cggggcgacc gatcgggcgg cggggaaacc cgcattatcg gcgcattgcc
     8101 cacgccagca ggacgaggga gcgcaaagaa cctgcggagc ctgcgtgtca actactatga
     8161 ttacgatagc tgctcgcgct tgccaggtga gcgctggagg catattctgc gcatgaaaca
     8221 tcggcccagt tcagcaaccg cacgccgcca gcccgtatct ggacaggtgg gcgccgggtt
     8281 cgccggcggc gctgcaccgc ggccggtcag gcgttgacca ggatgactcg aaagtcgttg
     8341 acgttggtca gggtgggccc ggtgaccacc gcatcgccca gcgcctgaaa gaagccatgg
     8401 gcgtcgttgt cgtccaggct gcggcgcgga tggatgcctt gcgcgaaggc gcgtgccagg
     8461 gtgtccgggc cgatgtgcgc gccggcgacc tcctgcgcgc cgtccacgcc atcggtgtcg
     8521 cccatcaggg cgtggatgcg cgcgtgaccg cgcgtggcga tggccagcga cagcaagcac
     8581 tcgacgttgc gtccgccgcg cccctgaccg cgcagtgtga cggtggtctc gccgcccgac
     8641 aggatggcgc aaggggccgt gaacggctgg ccacgcccgg ccacctgcag cgccagcgcc
     8701 gccagcacct tgccgacctc gcgcgcctcg ccttcgacgg cgtcgctcag gatgtgcgcg
     8761 gcgtagccat gccgccgtgc cacctgcgcc gccacctcca gggcctgctg cggagccgcg
     8821 accatgcgcg tggtgtggcc cgccagtcgc gcatcgtcgg gcttgacgga ctcgccgcgc
     8881 ccgctgtcca gcagcacacg caccggcgcg ggcaactcga tgccgtaacg ctgcacgatg
     8941 tccagcgcat cggcgcaggt ggtgggatcg cccaccgtgg gaccggaggc gatgtccatc
     9001 ggcacgtcgc ccggcacgtc cgacagcacc agcgtgatca cgcgcgccgg gtggcaggcc
     9061 gcggccaggc gcccgccctt gatggccgac agatgccggc gcacggtgtt catctcgcca
     9121 atgctggcac cgctggccag cagggcacgg ttgacctgct gtttgtcggc cagcgtgatg
     9181 ccgggcaggg gcaagggcag caaggacgag ccgcccccgg agatcaggca cagcaccaca
     9241 tcgtccgggg tcaggcccga gacgaggccg agcatgcgct cggccgcctg cagcccggcg
     9301 gcatcgggca ccgggtgcgc cgcttcgacg atcctgatgt gctgacacgg cacgtggtag
     9361 ccgtagcgcg tcaccaccag gccatcgagc gggccgcgcc agtgctcttc caccgcgcgc
     9421 gccatcgcgg ccgacgcctt gccggcgccc accacgatca gtcgaccccg cacttcagcc
     9481 gggttcggca ggtgagcagg cacgcagcgc gccggttgcg ccgaggcgat ggcggcctcg
     9541 aacatctcgc gcatgaggcg aacgggctcc atggcctagg gcctgttcac gctatgcaag
     9601 tggactgggt gcaagatgcc cacaccatgc cgaagcggcc ccggcctgcg ctgcggcatg
     9661 acatgcaatg cctttgccgc aacgacacgc tgccggcacc cggcggcacc aggccgcgcg
     9721 gcggcgaaac gctgcgcaaa cgcgggttcg cgaaagcgtc ggttcaggat cgctgcaggc
     9781 gggggcatgg aagcatcctg gcaagggccc agcggtgaag accccccatc ttgccagagg
     9841 ccggccgctc gcacccgacg cggctgccga tccgggtcac gcgcagcgaa tcgccacccc
     9901 aaccggccat cggccgccct ggcgcagctg aggccgggcg ggtcgctgcc gcacgttcgg
     9961 gcatcagggc gccaggcgtt cgcgcaccca gccgcccgct tccagtcggt agcacagacg
    10021 gtcgtgcagg cggctcttgc ggccttgcca gaactcccag cggtccggcc tgaggcgaaa
    10081 gccaccccaa tgcggcggtc tgggcgggtt gagcaaaaac ctggcgccga ccttggccgc
    10141 ctcggcagcc agccaggcac gtccgggaat gacctggctt tgcgggctgg cccaggcccc
    10201 caggcggtgg tccaacgggc ggctggcgta gtaggcatcg ctttcagcct cgctcacctg
    10261 ctccaccagg ccttcgatgc gcaccacccg ttccagttcg acccagtgaa actgcagcgc
    10321 ggcgtacggg ttgccggcca gctgacgccc cttgcggctt tcgtagttgg tgtaccagac
    10381 gatgccgcgt tcatcgaatt ccttgatgag caccacgcgc gtggaaggcc gcaagtcgct
    10441 gcccacggtg gccagcgtca tggcattggg ctcgggcaac tcgctctggc gtgcctcctc
    10501 gaaccactgg gcaaactgcc gacccggatc ggcgtgcgag gcgtcctcgg acagctcggc
    10561 acgctcataa ctcttgcgca gatgagcgat gtctttcatc cgcggcagta taggcagatc
    10621 atgcgccgtc ccgtcccgga agctcgaagc gcatgccgcg tgcccggcgc gcccgatcca
    10681 ggcgccaggc gcagccgcag caccggccac ctcccgcgtg tgttccggga ggtccgagcg
    10741 ggcatcaggg acgttcgccc ccgctcacgc ctcttcatcg tcgaccaggc tcagcagcgt
    10801 gcgcaggcgg cgatcgtgga tgccatgggc gcccagggtt tccagcgtgc gacgggcata
    10861 gtccagcgtg gtgccgtagc gcccgcgggc atggcggaag atgtggcgat actgctctgg
    10921 cgtcagcgcc cccgtgaagg agggactgcg gcgcgacagg gtgaacgcca gcgcccacac
    10981 gatgccgtgc ggcgtggcac agggcagcca gcgcgcgtcg tacacgccgc tgggcatctc
    11041 gcgcgcccaa agcgtgtcga acaccgcatc ggccgtgccg cgcggcaccc gaaacaccat
    11101 gccctggcag cttccgcccg gcaacagcgc gaacaccagc cccggttgct cgggcgtgcc
    11161 gcggttgacg cggctccaca tggccagcgc gcggtgccag ccatgcacgc gggtggcatg
    11221 gcgctcactg aagtcgaagt ccggccgcca gatcagcgag gcatagccga acacccacag
    11281 atcggcttgc ggaccggcgc gacggtgcca ctgggcacgc gtgtgctcca gcagccgcag
    11341 cggatcgcgc ccaggggcgc gcaggtgcgc aaagtcggcg taagcaggcg gcagcggtgc
    11401 gtcggcggag cgcatgacgg gaatttatac aatggacgcc atttttctgg agcccatcaa
    11461 tgagtgaaca aagggaagaa gagcgcggcg tcgtctgggc cgtgctgatc ggtgtggtgc
    11521 tgctggccat cggcctggcc gtcggcatgg ggctgtaccg cacgggcaag ggcgtggctc
    11581 ccgcggccgc ctccagcgcg gccaacgcca acgccaatgc aggcggcacg gctgccggcg
    11641 gtgcggcggg tgctccggcc aacaccgcgg ccatgaccag tggcgaggtg ggcgatggcg
    11701 cagcgatccg cgtcgagaac ggcgtggtca agttctactt cgccagcggc agcgccgaac
    11761 tcgcggcagg tgctgccgaa gcgctgggcg aggtcgtcaa aggcgtggcc gccggcaaga
    11821 aggcggtgat cagcggcttt cacgacgtca ccggcgaccc ggtcaggaac gaggagctgg
    11881 ccaagcagcg tgcgttggcg gtccgcgacg cgctggccgc actcggcatc ggcgaggaca
    11941 agatcgacct gaggaaaccc gccgtcacca ccgccagcgg cagcaacgcc gaggcgcgcc
    12001 gcgtcgaagt ccacctcgag tgacaactca aagcggcagt tgaccgcttg tcttcttcgg
    12061 gcgggccgca tcgatgcggg gtttcgcggc tccgatggcg ttgccttcac ctgttgggtg
    12121 accacgctac ccacccgagg gtcgcggccc atactggccg cggctcaggc tgcggctggg
    12181 cttggcgtct ggatcggcct cttgggtgcg cgaccctcgg gcacgcggaa cgcacccccg
    12241 ccttctgagg cgacggcgcc gcagacgccc ccgcgcacga aacccggccc gacccaggcc
    12301 gggttttttt tcatgcgccg gcgaacacga cgctccggca agcgcgccgc gctattcgta
    12361 caccaccatc gcccggcctt cgtcggcctg tgccgtccag ctggccagcg ccgcatcgct
    12421 gggaaagaat tgcgcctgct cacccaggtg caactgggcc atgacgccac cttccgagcc
    12481 gcgccgctcc agcagcaggc gcacgggcag accacgcagc agctcgccgt gctcggtgtg
    12541 ctccacctgg ggcgggtact cggccagcag gcggcgcacc tcgggtgcct tgccggccac
    12601 cgccacgcgc aggtacttgc cgaaccggca gcgcgcctgg gccaggtccc agatctgggt
    12661 gacgttcaac tgcagaccgc cggagaagcg gtcgggcatg gccttgccca tgacgacgat
    12721 gaattcgtcg tccttcagga tgtgacggtg tcgatggatc agcgcttcct cggcccgcgc
    12781 ctcgatcacg cccgacttgt cgtccaggcg aaacagcgcc agcttgccac gctgtccgtt
    12841 gatgatgcgc agatcgccca cgatgccggc cagcaactgc ggttcgcggc tgtcggccag
    12901 ttcggcgatg gaacggcgcg cgaactgccg cacctcgcgc tccacctcgt cgaacagatg
    12961 gcccgacagg tagaagccga tggccgtctt ctccagcgac aggcgctcct tcacacccca
    13021 gggcgtgacc gccggcagtt cgggctcctg tgaactggag ccgtgaccgt cttccatcag
    13081 gtcgaacaac ccgccctggt ggacattggc ttcactggcg gcggcgaaat cgaacgccag
    13141 gtcgatcgag gctgccagcg ccgcgcggtt cagctgcagg gcatcgaatg ccccggcgcg
    13201 aatcagcgcc tccaccgtgc gcttgttgac gcgcgtgcgg tcgacccggc ggcagaaatc
    13261 gaacaggctg gtgaatggtc cgcgttctgc accggcgggg cactcgccgc gcccttctcg
    13321 tgcggcgacg atggcttcga tggcctgctg gcccgtgccc ttgatggcgc ccagcccgta
    13381 gcgaatcagg gtgttgctga ccggctcgaa gcgatagccg ccacggttga tgtccggaga
    13441 ctcaaagcgc atgccctcgc gctgggcgtc ctcgtacaac accttcagct tgtcggtgtt
    13501 gtccatctcc acggtcatgt tggcacagta gaactcggcc gtgtagtgca ccttcagcca
    13561 agccgtgtgg taggccagca gcgaataggc ggcggcgtga gacttgttga aaccgtagcc
    13621 agcgaacttc tccatcaggt cgaagatctc gtcggccttg ccctcggaga taccgttcct
    13681 ggccgcgccc tcgcggaaaa tctggcggtg ctgcgccatc tcttcgggct tcttcttgcc
    13741 catggcacgg cgcagcaggt cggcaccgcc caggctgtag ccgcccagga tctgcgcggt
    13801 ctgcatgacc tgctcctggt agaccatgat gccgtaggtc tccgacaaca tgtcggccac
    13861 cagcgggtgc ggatacacca cttcctcgcg cccgtgcttg cgcgccacga agctggggat
    13921 caggtccatc ggccccggac ggtacagggc gttgagcgcg atcaggtctt ccagccgcgt
    13981 cggtcgcgcg tcgcgcagca tgccctgcat gccgcggctt tcaaactgga acaccgcttc
    14041 ggtgcgcccc tgagcgaaca gatggtaggt tggcgcatcg tcgagcggca ggttttcgta
    14101 ggcgaagtgc tgctgacccg gatgccgttc gacgatcagc tccttggcga tttccagaat
    14161 ggtcagcgtg gccagcccca ggaagtcgaa cttcaccagt ccgaccgcct ccacgtcgtc
    14221 cttgtcgtat tggctgaccg ccgcctcgct gccgggctgc tggtagagcg ggcagaagtc
    14281 ggtcagcttg cccggcgcga tcagcacgcc gccggcgtgc atgccgacgt tgcgcgtcat
    14341 gccctccagc ttctgggcca gctcgatcag cgtgcgcacc tcgtcttcct tgcggtagcg
    14401 ctcggccagc aggggctcga ccttcatggc gccatcgatg gtgatgtgct ggccgggctt
    14461 gttggggatc agcttgctga tgccgtcgca gaaggtgtag ctcatgtcga gcacacgtcc
    14521 gacgtcgcgg atcgcggcgc gcgccgccat ggtgccaaag gtggcgatct gactcactgc
    14581 gtcccggccg tacttgtcct tgacgtaatc gatcacgcgg tcgcggttgg cctgacagaa
    14641 gtcgatgtcg aagtcgggca tcgacacccg ttcggggttc aggaaccgct cgaacagcag
    14701 cttgtactgc agcggatcga ggtcggtgat cttcagcgcg tacgccacca gcgagccggc
    14761 acccgatcca cgacccggcc ccaccgggca gccatggttc ttggcccagt tgatgaagtc
    14821 gcccacgatc aggaagtagc ccggaaagcc catcttcagg atggtgtcga tttcgaactc
    14881 cagccgttgc aggtagcgcg gtcgctcggc gtcacgctgc gacgggtcgg ggtacaggtg
    14941 cgccaggcgc tgctccagcc cgtcagtgga agtctggcgg aaataggttt ccaccgtgtg
    15001 cccttgcggc accgggaaat ccggcagacg gggcttgccc agcgccaacg tgaggttgca
    15061 gcggcgcgcg atttcggccg cgttgctcag cgccgcgggc aggtcggcga acagttgctg
    15121 catctcggcc gtggacttga aatactgctc cggcgtgaag cggcgcacgc gccgcgggtt
    15181 gcccagaatc tcaccctcgg cgatgcacac cctggcttcg tgcgcctcgt agtcgtcggc
    15241 acgggcgaac tgcaccgggt gcgtggccac cacgggcaac ttcaagcggg cggccagctg
    15301 cacggccgcg gccacatgcg cctcgtcgtc ggcgcgccct gcgcgctgca gctccaggta
    15361 gaagcggtgg gggaacagct cggccagttg cagggccagc gcgcgggcct gttccacacc
    15421 gccctgcgcc agggcctgcc cgaccgggcc cgcctgcgcg cccgacagca ggatcaggcc
    15481 gtcggccagc tcgcccagcc aggcccagcg caccaccgcc tgccccttga ccacgccctg
    15541 ggtccaggca cgcgccagca gctccgacag gttcaggtag ccctgcccgc tggtcaccag
    15601 cagcagcacg cgggtgggcg cttccgggtc cgacccctgc ccttccagca gcacctcggc
    15661 accgatgatg ggcttgacgc cagcgccgcg cgcggccttg tagaacttga tggcgccaaa
    15721 caggttgccc aggtcggtga tggcgagtgc gggctgagcg tcgtcggcag cggcctggac
    15781 gatgtcgtcc aggcgagtcg tgccatcgac gacggagaac tcggtgtgca ggcgcagatg
    15841 aacgaacatg ccccgcattg tcgggcaagg cacgggcggc ctgccgcgca cgggtggtcc
    15901 gaggcggcgg ccgggcgggc acggtgtcgc agctggcggc tcagcacgcc ggagcgcgcc
    15961 cggatcgaac cccgcccgag cgtgtagagt tgccgcatga cgccccctcg cccccacctc
    16021 tcgcaccgca tcgccgtcgt tggcgccggc attgccggac tggcctgtgc ccgcaccctg
    16081 cgacaggccg ggcacgaggt cgccgtgttc gaagccgctc cccaaccggg cgggcgcatg
    16141 gccaccgtgc agaccgcctt cggcggcttc gacgccgggg tgcagtactt caccgtgcgc
    16201 gacgaacgct tcatgcaggc gttgcaggcc acggcatccg acgtggtacg acgctggagc
    16261 gccaacgccg tgcgtgtgct cgacgaacgc ggccgcgtgg tggaggccgc cccgccggcg
    16321 cccgaatctc actgggtggc cgtgcccgcc atggatcgcc tgccccagcg ctgggccgag
    16381 ccactggttg cggccggcgc gctgcacctg gacacccgcg tctggcgcgt ggcgcacgac
    16441 cggctctcgc cagggcgctg gcagctgcat accgacggcc cgggcgaaga ccatccggtg
    16501 caggccggct ttgaccaggt tctgctggcc atcccgcccc agcaggcgaa cgagctgttg
    16561 caggcttcga ccctcagcac agcgccgttc gctgccctgg gagcggtcga cgtggcgccg
    16621 tgctggaccc tgatgctggc ctaccccaac gccacgcagc cagggctgca cacgctgggc
    16681 ccgcagtgga atgcggcgcg cagcacgcac caccgcgtca gttggctggc gcgcgaatcg
    16741 tccaagcccg ggcgcgtggc ggtcgagcgc tggacggtgc aggccagcgc cgcctggtca
    16801 cgcgaacacg agcgcgacga cccggcgcgc gtcagcgcca agctgctgcg cgcctttgct
    16861 gaaatcaccg gcatccatgc cacgccggcg cacaccgaag ccctgctgtg gcgccaggcg
    16921 cgcaccctgg cgccgctggg ccgcacgcac ctgtgggacg gcgcaggcag catcggcctg
    16981 tgtggtgact ggtgcctcgg ctgccgggtc gaagacgcct tcgtttccgg gctggagctg
    17041 gcgctggccg tgatcgccgc ctgaacgggc cgatcgacgc cgggccccga cgccctacgc
    17101 cgccatgtac gtcggccgct tcgccccttc acccaccgga ccactgcacg ccggctcgct
    17161 ggtggcggcg ctggccagct ggctggacgc acgcgcccac gacgggcgct ggctggtgcg
    17221 tatcgaggac gtggacacgc cgcgctgcgt gcccggcgcc gatcggcaga tcctgcagca
    17281 gctggccgcc tgtgggttgc ctcccgatca accgccggtg tggcaatccg ggcgcagcgc
    17341 gcactaccag gccgcgctcg acgcgctggt caccgccggc gcggcctacc cctgcggctg
    17401 ttcacgcaag gacatcgagc aggcgcaggc cgcgcgcggc ctcaggcgcc agcgcggggt
    17461 ggagctgccc taccccggca gctgccgccc cgagcgcggc ggactgcaag gcaagcctgc
    17521 gcgggcctgg cggctgcaca ccggagttca tgaatcaaat ccacctccag ggcaatccgg
    17581 gcaagcgcca gcagctatcg ttagtgaagc aaccaacgag gccattgtct ggcacgaccg
    17641 gcgcctgggg ccgcaacggc aggatgtggc gcatgaggtc ggcgacttcg tgctgcgccg
    17701 cgccgacggc ctgtgggcct atcagctggc ggtggtggtg gacgacgccg cccagggcgt
    17761 gacccacgtg gtgcgcggcg ccgacctggc cgacaacacg ccgcgccaga tcctgctgca
    17821 gcgcaggctg ggcctggcca caccacgcta cctgcacaca ccgctggtgc tggccgccga
    17881 cggcgaaaaa ctcagcaagc agcatggtgc gcaagcgctc gacgtgtccg acccgctgcg
    17941 gacgctgcaa cgcgccgcag cggtgctggc actggacccc aaggccggca cctcggtgcc
    18001 cgaagcgctt gccgcctgga ccgcccagtg gcggcagcgc tgggtgcagg ccgactgagc
    18061 ctagtgctga gccgacggcg cacaaagaga ttgagccgac ggcgcacaaa gaaaaggccc
    18121 gacctgacgg ccgagccttt gaatttggtg ggtcgtgcag gattcgaacc tgcgaccaac
    18181 ggattaaaag tccgctgct
//
LOCUS       DAOOOG010000033        17773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_3287_length_17773_cov_25.275878, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000033 DAOOOG010000000
VERSION     DAOOOG010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 17773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17773
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3287_length_17773_cov_25.275878"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..521)
                     /locus_tag="PK242_04370"
     CDS             complement(192..521)
                     /locus_tag="PK242_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOZ93284.1"
                     /translation="MTHVVTEACINCKYTDCVDVCPVDCFKEGPNFLTIDPDECIDCA
                     VCIPECPVNAIFAEEDLPKDQLHMTQINAELARSPNYRTLTKRKDPLPDHEAWASRTD
                     KLPELVR"
     gene            complement(883..2373)
                     /locus_tag="PK242_04375"
     CDS             complement(883..2373)
                     /locus_tag="PK242_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HOZ93285.1"
                     /translation="MSTTAVHPVDERLPTGKLTALGLQHVLVMYAGAVAVPLIVGRAL
                     KLGPQEVAFLISADLFCCGIATLIQALGATQWFGIRLPVMMGVTFAAVGPMVAIANGN
                     PGTDGARMLFGAIIGAGIISILIAPVVSRMLRFFPPVVTGTIIAVIGINLMRVGINWI
                     FGNPVGPTAPLTLSPEHTEWLSTMRSAGATGQPGVLPVPQGLTLAPTVPNPNYAKLSG
                     VAVSAIVLASILLIAKFAKGFIANISVLLGIVIGGIVATATGMMNFDKVGKAAWFDVV
                     TPFHFGTPIFDPVMILTMTLIMIVVMIESTGMFLALGEMTDRRIDQPMLSRGLRTDGL
                     GTLIGGIFNTFPYTSFSQNVGLVAVTGVKSRFVCVAGGLILIVLGLLPKMAALVESLP
                     TVVLGGAGIVMFGMVAATGIRILGGVDYTHNRNNALIVAISIGVGMIPLVAPRYLQWM
                     PHAIHPLIESGIVLASIAAVLLNLFFNGARADTEASIRAARQAEAH"
     gene            complement(2425..3693)
                     /locus_tag="PK242_04380"
     CDS             complement(2425..3693)
                     /locus_tag="PK242_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOZ93286.1"
                     /translation="MTTATDTYARLDAWIDQHFDEQVRFLQQLVRVPTDTPPGNNAPH
                     AERTAELIAAWGFEAERHPVPEQEVRDYGMQSVTNLIVRRPYGKGGRTIALNAHGDVV
                     PPGEGWTHDPYGAEIEGGQMFGRATAVSKSDFSTFSFAVRALEAVAKPTRGTVELHFT
                     YDEEFGGLLGPGWLLAQGLTRPDLMIAAGFSYEVVTAHNGCLQMEVTVQGRMAHAAVP
                     HTGVDALQAAVFIMNALYAENSAYKKVSSKVPGIRHPYLNIGRIEGGTNTNVIPGKVV
                     LKLDRRMIPEEKPAKVEARIRKVIEGATRDFNRWRGVKGEDAVRTDVRRILLAHAMTP
                     LPGNRPLVEAIQKHGEAVFGARPPAVGTPLYTDVRLYVERGIPGVIYGAGPRSVLESH
                     AKRADERVDLQDLRRATKVIARALADLLRD"
     gene            complement(3748..5538)
                     /gene="uraD"
                     /locus_tag="PK242_04385"
     CDS             complement(3748..5538)
                     /gene="uraD"
                     /locus_tag="PK242_04385"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HOZ93287.1"
                     /translation="MPYTLDQLNAAPLAEAERMLDGLYEHTPWIAAQALQARPFQSLA
                     QLKQAMAGALTRASVDAQLALIRAHPELAGKAMEAGTLTSESTGEQQRAGLTACTPEE
                     LARIRQLNADYQARFGFPFVLCVRGPRGTGLAKQQILDTFERRLHHHPDFERAEALRQ
                     IHRIVEIRLADKFGAPPTLGEQVWDWHEKLAQHSDPGCAEAGQLTVTYLTDAHRACAQ
                     RISRNMRDAGFDDVHIDAVGNVVGRYKADPGARNPKTLLTGSHYDTVRNGGKYDGRLG
                     IFVPLACVQELARTGRRLPFDLEVVGFAEEEGQRYKATFLGSGALIGDFRPEWLDQRD
                     ADGISMREAMQHAGLCVDDIPKLKRHAADYLGFIEVHIEQGPVLNELNLPLGVVTSIN
                     GSVRYQGAYIGQASHAGTTPMDRRRDAACAAAELALYLERRAAQDGDSVGTIGMLTVP
                     NGSINVVPGRCNFSLDLRAPSDAQRDALVSDVLAEAQAIAERRGVRLQLEETMRAAAA
                     PCDAALQQRWADAVEALGLPVFRMPSGAGHDAMKLHEIMPQAMLFVRGENAGISHNPL
                     ESTSAEDMALAVHAMQHLLDGMARDTLSQS"
     gene            complement(5545..6501)
                     /gene="puuE"
                     /locus_tag="PK242_04390"
     CDS             complement(5545..6501)
                     /gene="puuE"
                     /locus_tag="PK242_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HOZ93288.1"
                     /translation="MTRPSYDTTAAYPRDLAGYGRDAPHARWPGGARLAVQFVLNYEE
                     GGENCVLHGDAASEQFLSEMFNPAAYPDRHLSMEGIYEYGARAGVWRILREFDARGWP
                     LTVFGVASALARQPEVLRAFQARGDEIACHGLKWIHYQNVDEATERAHMAEALALIER
                     LSGTRPLGWYTGRDSPNTRRLVVDDGRLLYDSDYYGDDLPFWMKVRRSDGAVVPHLVV
                     PYTLDCNDMRFALPQGYSHAEPFFQYMKDSFDALYAEGDPAGLDRPKMLSIGMHCRLL
                     GRPGRITALQRFLDHVARHPDVWVCRRIDIARHWIATHLPQD"
     gene            complement(6507..7205)
                     /locus_tag="PK242_04395"
     CDS             complement(6507..7205)
                     /locus_tag="PK242_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOZ93289.1"
                     /translation="METLSSTDAIVQALTHAIVDHRLQPGSKLGEQALADHFGVSRTL
                     VRQALFQLSQKRLVTLAPARGAFVASPSADEARQVFAVRRMLELELTRAFVRQASPAD
                     IDALRAHVAAEQAAVAGGDVAGRTELLGDFHVRMAELLGNAVLAEVLRDLLARCAIAI
                     LMYQSSHAAHDSAAEHAALVDCFAQGNVTQAVKLMRAHLDHVEAGLQLDQPAPTNDIR
                     TALMPTSQAPAAIV"
     gene            7279..7632
                     /gene="uraH"
                     /locus_tag="PK242_04400"
     CDS             7279..7632
                     /gene="uraH"
                     /locus_tag="PK242_04400"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HOZ93290.1"
                     /translation="MGLSTHVLDTMHGTPAAGMAVALYETHGEVATLLRRFTLNADGR
                     NPDGPLFDNASMKVGTYRLSFDVAGYFRARGVALPQPPFLDQVHLDFGIAQVDQHYHV
                     PLLCSPWSYSTYRGS"
     gene            7715..8530
                     /gene="xdhC"
                     /locus_tag="PK242_04405"
     CDS             7715..8530
                     /gene="xdhC"
                     /locus_tag="PK242_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HOZ93291.1"
                     /translation="MRELAELLARLPQAPAALVTVVRSAGSVPRELGAWLAVFGDGVV
                     LGTIGGGHLELEAIERARAALAAGPFEPEERRCALGPSLGQCCGGVVWLRQERIEAAH
                     IEALRTRLMPATRPLALFGGGHVGRAIVRLLEELPFAVRWVDSRDEVFPAVLPLNVQA
                     EHSDPVQAAVAELPAGSAVLVMSFSHAEDLEVIAACLARQRERGDLSHIGLIGSRTKW
                     ARFGHRLAERGFSQAELARVSCPIGLPEIRGKQPAVVALAVVAQLLALGLAPG"
     gene            complement(8723..8881)
                     /locus_tag="PK242_04410"
     CDS             complement(8723..8881)
                     /locus_tag="PK242_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93292.1"
                     /translation="MGNKIVTEAMRKATPPLPGAPRVKQADGQRRSLERGVHTRSKKN
                     PGKRPHQG"
     gene            9145..10353
                     /locus_tag="PK242_04415"
     CDS             9145..10353
                     /locus_tag="PK242_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HOZ93293.1"
                     /translation="MEAYLLDWANLLLRWLHVITAIAWIGSSFYFVFLDSSLLAPTAD
                     DLKAKGVDGAIWSVHGGGFYHAQKYMVAPKGGIDSHLHWFYWESYWTWISGFLLFVVL
                     YLWNAGIFLVDKQVFDWSPLAAGIGALVFLAAFWFIYDAICRIFGQRANGDRIVGALV
                     LAVVVCSSWIAFQLFSGRAAFLMVGASLATAMSANVFFWIIPGQRKVVAAMTSGQPYN
                     PADYAIYGKRGKQRSVHNTYFTLPVLVAMLSNHYSFIYSHPQRWAILLVLMLAGALIR
                     LFFVQRHGYKLGRVGNPWPYAAAGVVLLLALIAWLKPAPAVPAAALPAKVSYAQIQPI
                     LAQRCYVCHGEAVQQKNLRVDSEALVSANAQLIYQQVVASKLMPLANSTQMTEDERAL
                     VGRWFQGQGK"
     gene            complement(10403..10651)
                     /locus_tag="PK242_04420"
     CDS             complement(10403..10651)
                     /locus_tag="PK242_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOZ93294.1"
                     /translation="MSIIVTLVVGFIVGLIARAIMPGNQSMGLIMTTILGIVGSLVAG
                     YGGAALGLYAAGTPAGWIASVVGAIIVLWIYGMVSKKG"
     gene            complement(10881..13022)
                     /locus_tag="PK242_04425"
     CDS             complement(10881..13022)
                     /locus_tag="PK242_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ93295.1"
                     /translation="MIQLSNVSLRRGARLLLDGASVTLHPGEKVGLVGRNGAGKSSLF
                     ALLSGALHEDSGDCAMPAGWRLAQVAQQLPETEDSTTDFVLQGDERLSELRRQLSRAE
                     QTQDGMAIAQAHSDLHDAGAHDARARAEALILGLGFQPHELARPVNRFSGGWRMRLQL
                     ARALMAPSDLLLLDEPTNHLDLDALVWLEGWLARYAGTLIVISHDREFLDAVTRVTLH
                     IEGGRLTRYGGNYSAFEDLRAEQLSQQQAAWARQQDKIAHLQKFIDRFKAKATKARQA
                     QSRVKALERMERIAPVLAEAEFQLEFKAPLSLPNPMLAIRDAAFGYRADAIKKEAAGA
                     DPASPSGASGTESAAPPAYATSATVLAEQAARGELPPGLRPGDRVILRGVSRTVLAGQ
                     RIGILGANGQGKSTLVKTIARELAPLAGSVTEGKGLAIGYFAQQELDVLHTDSTPLEH
                     MVRLARQSGREASEEAREQQLRNYLGTFNFPGDMVNQPVGQFSGGEKARLVLAMIVWQ
                     RPNLLLLDEPTNHLDLATREALAVALNEFEGTLMLVSHDRALLRSVCDEFWLVARGSV
                     QPFDGDLEDYQRHLLDEARRLRERVVACAPETIADGASQESARAPKPAEPPLNPAEQR
                     RAQAQRRQQLAEQTRPLRRALAQAEQRMEQLGAERSALEQRLSSPLPAGDIADAGRRL
                     KALAEELQTQEDEWLRLSGEIEAIEQGAAAT"
     gene            complement(13019..13891)
                     /gene="prmB"
                     /locus_tag="PK242_04430"
     CDS             complement(13019..13891)
                     /gene="prmB"
                     /locus_tag="PK242_04430"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOZ93296.1"
                     /translation="MTTLTQLITDTERQLSAAGVAFGHGTTNARDEAAWLVLHALGLP
                     LDTDLSGSEGNQPVSPAGQAQAAMLLKARIDTRQPLAYLTGQAWLQGVPFHVDARAIV
                     PRSLIAELIADGGIDPWLSERTRRVLDLCTGNGSLAVLAALAWPEVQVDAADLSRDAL
                     AVARLNVERHGLQQRIRLIESDGLAACPGPYDLIVCNPPYVNAASMAALPPEFRAEPA
                     LALAGGADGMDFIRPLLRQAPAHLNAGGVLVLEIGHERAHFDAAFPQLQPVWLDTSAG
                     DAQVLLLTREALAP"
     gene            complement(13944..15131)
                     /gene="dapE"
                     /locus_tag="PK242_04435"
     CDS             complement(13944..15131)
                     /gene="dapE"
                     /locus_tag="PK242_04435"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HOZ93297.1"
                     /translation="MTATRRLAEALIARPSVTPADGGCIDLIAERLAPLGFEPEFIDR
                     GPPEWRVRNLWSKRVPAQYQQAPQAIETIVFAGHVDVVPTGPVEAWTSPPFTPTERAG
                     RLYGRGASDMKGAIAAFVVALEEFLAARPEAPLALALLLTSDEEGRALDGTRVVCEWL
                     AARGERLHACIVGEPTAQRHSGDMVKNGRRGSLSGRLVVRGVQGHVAYPHLARNPIHQ
                     LAPALAELAATAWDAGNAHFQPTGWQVSNLHAGTGAGNVIPGQAVVDFNFRYGTASTP
                     ASLQARLQAVLQRHGLDHTLEWTLGAEPFLTPPGSLLDAVQAAIAAETGLQPELSTTG
                     GTSDGRFLARICPQVIELGSPNASIHQIDEHIELAELERLKNIYRRVLEQLAAQARVA
                     QLR"
     gene            complement(15315..16586)
                     /locus_tag="PK242_04440"
     CDS             complement(15315..16586)
                     /locus_tag="PK242_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOZ93298.1"
                     /translation="MPKPVASTTMMDRILHLMAEKKASDVYLSAHAPATIRINGVSMP
                     INNQVLPPEATLNLLMDVVPPNRIEELKETGELNMAIPIYGVGNFRLSAMRQRGTYAA
                     VIRFISPEIPDLDSLNLPSILKHLVMEKRGLVLMVGATGNGKSTTLAAMLDHRNRNAT
                     GHILTIEDPIEYVFTNHKSLINQREVGTDTAALQIGLKNALRQAPDVIMIGEIRDRET
                     MTAAIAYAQSGHLCLATLHANNSYQALNRVLSFYPVEVRPTLLGDLSAALKSIVSQRL
                     VRTTEGSRVPAVEVLLNTKLVADLIEKSDFSGVKDAMDNAIAEGSQTFEQDLARLILE
                     NRIDRKEGLANADSPTNLMWRLQNQTVRHLARKPEEEERPSDEPSFTDITLDVRPSGF
                     GKSGFSRSGYRDSGFQDSKVGSFSDSHIGDA"
     gene            16937..17488
                     /locus_tag="PK242_04445"
     CDS             16937..17488
                     /locus_tag="PK242_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93299.1"
                     /translation="MIYAKSESGLQAVKDRHAVDLTRAQRSVLILIDGKRSVREVLEA
                     TATLGVTATDVELLVQRGLVVVASGGMSLNGSAGGASLNGSSGGAGRVTATGDGAASG
                     SGDPAAPDLTDDERARRYRVAYPMATQLTAKLGLKGFRLNLAVEEAAGFEGLVDLLPR
                     LRSALGSEGVRPLESVLIGDPLQ"
BASE COUNT         2566 a         5765 c         6440 g         3002 t
ORIGIN      
        1 gcttgcggcg gcgttgcgcc tcttgccaag gccgccagca tgggctgcga gccgcacctt
       61 gccgcaagcc ttgttctggc ttctgtaagt gcctgacagc gcgagacact gcaccggtac
      121 cccaaaaaca ggagcggccg gcgcaggccc agcctgcgcc agccgccatt ctgatgcaga
      181 cattcagggc gtcagcgcac cagctcgggc agcttgtcgg tgcggctggc ccaggcttcg
      241 tggtcgggca gcggatcctt gcgtttggtc agcgtccggt agttgggcga acgggccaac
      301 tcggcgttga tctgcgtcat gtgcaactgg tccttgggca ggtcttcctc ggcaaagatg
      361 gcgttgaccg ggcactcggg aatgcagacg gcgcagtcga tgcactcatc ggggtcgatg
      421 gtgagaaagt tggggccttc cttgaagcag tcgacggggc aaacgtccac gcaatcggtg
      481 tatttgcagt tgatgcaggc ttcggtgacg acgtgggtca tggtcagaac tcgtgggcaa
      541 agcgtgaaag caggcaggcg ccggtctgaa tgctcagcgc cggcacctgc agccatgcgc
      601 gaattttaag agggggcatc ggcggccggt cgccgtgcgg ttttggacgg tggtctgact
      661 gccggtcact tgatgcaagt caattggcga acggtcgaaa ttgcccggcg ggctgcccgc
      721 gcactgtagc caccctgcgg cccggatcca accattcgtt tgttctggcc atatgcgcca
      781 gcggcataag cgcgccaggt ggttgcctaa ccgtcggcac acgaacgaaa ccggccaccg
      841 cgggccggtt cgtgggttgg ggcagcgctc agccagcgag catcagtgcg cttcggcctg
      901 tcgggcggcg cgaatggaag cttcggtgtc ggcgcgggcg ccgttgaaga acaggttcag
      961 cagcacggcg gcgatggagg ccagcacgat gcccgactcg atcagcgggt ggatggcgtg
     1021 cggcatccac tgcaggtacc tgggcgccac cagcgggatc atgccgacac cgatggagat
     1081 cgccacgatc agcgcgttgt tgcggttgtg ggtgtagtcc accccaccca ggatgcggat
     1141 gccggtggcc gccaccatgc cgaacatcac gatgccggcg ccgcccagca ccacggtggg
     1201 cagcgactcc accagcgccg ccatcttggg cagcaggccc agcacgatca ggatgaggcc
     1261 gccggccacg cagacgaagc ggctcttgac gccggtgacc gccaccaggc cgacgttctg
     1321 cgagaagctg gtgtagggga aggtgttgaa gatgccgccg atcagcgtgc ccagaccgtc
     1381 ggtgcgcagg ccgcgcgaga gcatcggctg gtcgatcctg cggtcggtca tctcgcccag
     1441 cgcgaggaac atgccggtcg attcgatcat caccacgatc atgatcagcg tcatggtcag
     1501 gatcatcacc gggtcgaaga tcggcgtgcc gaagtgaaac ggcgtcacga cgtcgaacca
     1561 ggcggccttg ccgactttgt cgaagttcat catgccggtg gcggtggcca cgatgccgcc
     1621 gatgacgatg cccagcagca ccgagatgtt ggcgatgaag cccttggcga acttggcgat
     1681 cagcaggatc gaggccagca cgatggccga caccgccacc cccgacagct tggcgtagtt
     1741 ggggttgggc acggtcggcg ccagcgtcag gccctgcggc accggcagca ccccgggctg
     1801 gccggtggcg ccggccgaac gcatggtgct gagccattcg gtgtgctcgg gcgagagggt
     1861 cagcggtgcg gtcgggccga ccgggttgcc gaagatccag ttgatgccca cgcgcatcag
     1921 gttgatgccg atgaccgcga tgatggtgcc ggtgaccacc ggcggaaaga agcgcagcat
     1981 gcggctgacc accggcgcga tcaggatcga gatgatgccg gcgccgatga tggcgccgaa
     2041 cagcatgcgc gccccgtcgg tgccgggatt gccgttggcg atcgccacca tcgggccgac
     2101 ggcggcgaag gtcacgccca tcatcaccgg caggcggatg ccgaaccact gcgtggcgcc
     2161 cagcgcctgg atcagggtgg cgatgccgca gcagaacagg tcggccgaga tcaggaaggc
     2221 cacctcctgc ggccccagct tgagcgcgcg gccgacgatc agcggcacgg cgacggcgcc
     2281 cgcgtacatc accagcacat gttgcagccc gagggccgtg agtttgccgg tgggcagacg
     2341 ttcatcgacg ggatggacgg cggtggtgct catgttgtct cctgagggtt gtcgtcgttg
     2401 agcaagagaa gggggggcgg tcggtcagtc gcgcagcagg tcggccagcg cgcgtgcgat
     2461 gaccttggtg gcgcggcgca gatcctgcag gtcgacgcgc tcgtcggcgc gcttggcgtg
     2521 gctttccagc acgctgcgcg ggccggcgcc gtagatcacg ccggggatgc cgcgctcgac
     2581 atacaggcgc acgtcggtgt agagcggtgt gccgacggcc ggcgggcgcg cgccaaacac
     2641 cgcctcgccg tgcttctgga tcgcctcgac cagcggccgg ttgccgggca gcggcgtcat
     2701 ggcgtgggcc agcaggatgc ggcgcacgtc ggtgcgcacg gcgtcctcgc ccttcacacc
     2761 gcgccagcgg ttgaagtcgc gcgtggcgcc ttcgatgacc ttgcggatgc gcgcctcgac
     2821 cttggctggc ttttcctccg ggatcatgcg gcggtcgagc ttgagcacca ccttgccggg
     2881 gatcacgttg gtgttggtgc cgccctcgat gcggccgatg ttgaggtagg ggtgccggat
     2941 gcccggcacc ttggagctga ccttcttgta ggcgctgttt tcggcataga gcgcgttcat
     3001 gatgaacacc gccgcctgca gcgcgtcgac gccggtgtgc ggcaccgccg cgtgcgccat
     3061 ccggccctgc accgtcactt ccatctgcag gcagccgttg tgggcggtga cgacttcgta
     3121 gctgaagccg gcggcgatca tcaggtcggg ccgggtcaac ccctgcgcca gcagccagcc
     3181 ggggcccagc aggccgccga attcctcgtc gtaggtgaaa tgcagctcga cggtgccgcg
     3241 cgtgggcttg gccaccgctt ccagcgcgcg cacggcgaag ctgaaggtgg aaaagtcgct
     3301 cttgctcacc gcggtggcgc ggccgaacat ctggccgccc tcgatttcgg cgccatacgg
     3361 gtcatgcgtc cagccttcgc cgggcggcac cacgtcgccg tgggcgttga gcgcgatcgt
     3421 tcggccaccc ttgccgtagg gacggcgcac gatcaggttg gtgacgcttt gcatgccgta
     3481 gtcgcgcact tcctgctcgg gcaccggatg gcgctcggcc tcgaagcccc aggcggcgat
     3541 cagctcggcc gtgcgttcgg catgcggtgc gttgttgccc ggcggcgtgt cggtgggcac
     3601 gcgcaccagt tgctgcagga agcgcacctg ctcgtcgaag tgctggtcga tccaggcgtc
     3661 gagccgggcg taggtgtcgg tggcagtggt catggcgggg tcttgaatga aaaatggctc
     3721 tggcgcccga tgggtaagcg ccggcagcta tgattgtgat agcgtatcgc gcgccatgcc
     3781 gtccagcagg tgctgcatgg cgtgcaccgc cagcgccatg tcttcggcgc tggtggattc
     3841 gagtgggttg tggctgatgc cggcgttctc gccacgcacg aacagcatgg cctgcggcat
     3901 gatttcgtgc agcttcatgg cgtcgtggcc ggcgccgctg ggcatgcgaa acaccggcag
     3961 gcccagcgcc tccaccgcgt cggcccagcg ctgctgcagc gctgcgtcgc agggtgcggc
     4021 ggcggcgcgc atggtttctt cgagctgcag gcgcacgccg cggcgctcgg cgatcgcctg
     4081 ggcctcggcc agcacgtcgc tcaccagggc gtcgcgctgc gcatcgctgg gggcgcggag
     4141 gtccaggctg aagttgcagc gccccggcac cacgttgatc gatccattgg gcacggtcag
     4201 catgccgatg gtgccgaccg agtcgccgtc ctgcgcggcg cggcgctcca gatacagcgc
     4261 cagctcggcc gcagcgcagg cggcgtcgcg ccggcggtcc atgggcgtgg tgccggcgtg
     4321 gctggcctgg ccgatgtagg caccctggta gcgcacgctg ccgttgatgg aagtcaccac
     4381 gcccagcggc aggttcagtt cgttcagcac cgggccctgc tcgatgtgca cctcgatgaa
     4441 gcccaggtag tcggcggcgt ggcgcttgag cttggggatg tcgtccacgc acaggccggc
     4501 gtgctgcatg gcctcgcgca tgctgatgcc gtcggcgtcg cgctggtcca gccattcggg
     4561 gcgaaagtca ccgatcagcg cgcccgaacc caggaaggtg gccttgtagc gctggccttc
     4621 ttcctcggca aagccgacca cttcgaggtc gaacggcagg cggcgcccgg tgcgcgccag
     4681 ctcctgcacg caggccaggg gcacgaagat gcccaggcgc ccgtcgtact tgccgccgtt
     4741 gcgcaccgtg tcgtagtggc tgccggtgag cagggtcttg gggttgcgcg cccccgggtc
     4801 ggccttgtag cggcccacca cgttgccgac ggcgtcgatg tgcacgtcgt cgaacccggc
     4861 atcgcgcatg ttgcggctga tgcgctgggc gcaggcgcgg tgcgcgtcgg tcaggtaggt
     4921 gacggtcagc tggccggcct cggcgcagcc ggggtcgctg tgctgggcca gcttctcgtg
     4981 ccagtcccac acctgttcgc ccagcgtggg cggagcgccg aacttgtcgg ccaggcggat
     5041 ctcgacgatg cggtggatct ggcgcagcgc ctcggcgcgc tcgaagtcgg ggtggtggtg
     5101 caggcggcgt tcgaaggtgt cgaggatctg ctgcttggcc aggcccgtgc cgcgcgggcc
     5161 gcgcacgcac agcacgaacg gaaagccgaa gcgcgcctga tagtcggcgt tgagctggcg
     5221 gatgcgcgcc agctcctcgg gcgtgcaggc ggtgaggccg gcgcgctgct gctcgccggt
     5281 cgattcgctg gtcagcgtgc cggcttccat ggccttgccg gccagttccg ggtgggcgcg
     5341 gatcagcgcc agctgggcgt ccacgctggc gcgcgtcagc gcgccggcca tggcctgctt
     5401 gagctgcgcc agcgattgga acggtcgcgc ctgcagcgcc tgggcggcga tccagggcgt
     5461 gtgctcgtac aggccgtcga gcatgcgctc ggcttcggcc agcggtgccg cgttgagttg
     5521 atccagcgtg tagggcatgg gcggtcagtc ctgaggcagg tgggtggcga tccagtggcg
     5581 cgcgatgtcg atgcggcggc acacccacac gtccggatgc cgtgccacat ggtccagaaa
     5641 gcgctgtagc gcggtgatgc gcccgggacg cccgagcaga cggcagtgca tgccgatgct
     5701 cagcatcttg gggcggtcca gaccggccgg gtcaccctcc gcgtacaggg cgtcgaagct
     5761 gtccttcatg tactgaaaga acggctcggc gtggctgtag ccttgcggca gggcaaagcg
     5821 catgtcgttg cagtccagcg tgtagggcac caccaggtgc ggcaccaccg cgccgtcgct
     5881 gcggcgcacc ttcatccaga acggcaggtc gtcgccgtag tagtcgctgt cgtacagcag
     5941 ccggccgtcg tcgaccacca ggcggcgcgt gttggggctg tcgcggccgg tgtaccagcc
     6001 cagcgggcgc gtgcccgaca ggcgttcgat gagcgccagc gcctcggcca tgtgggcgcg
     6061 ctcggtggct tcgtcgacgt tctggtagtg gatccacttc agaccgtgac aggcgatctc
     6121 gtcgccgcgc gcctgaaagg cccgcagcac ctcgggctgg cgcgccaggg cgctggccac
     6181 gccgaacacc gtcagcggcc agccgcgggc gtcgaactcg cgcaggatgc gccacacgcc
     6241 ggcgcgcgcg ccgtactcgt agatgccctc catgctcaga tggcggtcgg ggtaggcggc
     6301 cgggttgaac atctcggaca gaaactgctc gctggcggcg tcgccgtgca gcacgcagtt
     6361 ttcgccgcct tcttcgtagt tcaggacgaa ttgcaccgca aggcgcgccc cgcccggcca
     6421 gcgggcgtgc ggggcgtcgc ggccgtagcc ggccaggtcg cgtgggtagg cggcggtggt
     6481 gtcgtaggag ggtcgtgtca tgctattcaa acaatagctg ctggcgcttg actggtgggc
     6541 atcaaagctg ttctgatgtc gtttgtgggg gccggctggt cgagctgcag gccggcttcc
     6601 acatggtcca ggtgggcgcg catcagtttc accgcctgcg tcacgttgcc ctgggcaaaa
     6661 cagtccacca gcgccgcgtg ctcggcggcc gaatcgtgcg cggcgtggct gctctggtac
     6721 atcaggatgg cgatggcgca gcgcgccagc aggtcgcgca gcacctcggc cagcacggcg
     6781 ttgcccagca gttcggccat gcgcacgtgg aagtcgccca gcaactcggt gcggccggcc
     6841 acgtcgccac cggccaccgc cgcctgctcg gccgccacgt gcgcgcgcag ggcgtcgatg
     6901 tcggccgggc tggcctggcg cacgaaggcg cgcgtgagct ccagctccag catgcggcgc
     6961 accgcgaaca cctgccgcgc ctcgtcggcc gacgggctgg cgacgaaagc gccgcgcgcc
     7021 ggcgccagcg tcaccaggcg cttctgggac agctggaaca gggcctgccg caccagcgtg
     7081 cgcgagacgc caaaatggtc cgccagcgcc tgctcaccca gcttgctgcc gggttgcagt
     7141 cggtgatcga cgatggcgtg ggtcagcgcc tgaacgatcg catcggtgct ggacagggtt
     7201 tccatggccc gccatgatag gcataattcc aaaaattgta tacaaccctg tgcacaatgc
     7261 attcaaggag tggttgatat gggactcagc acgcatgttc tcgacaccat gcacggcacg
     7321 cccgcggccg gcatggcggt ggcgctgtat gaaacgcacg gcgaagtggc gacgctgctc
     7381 aggcgcttca cgctcaacgc cgacggccgc aacccggacg gcccgctgtt cgacaacgcc
     7441 agcatgaagg tcggcaccta tcgtctgagc ttcgacgttg ccggctactt ccgcgcgcgt
     7501 ggcgtggcgc tgccgcagcc gccgtttctg gaccaggtgc acctggactt cggcatcgcc
     7561 caggtcgatc agcactacca cgtgccactg ctgtgcagcc cctggagcta ttcgacctac
     7621 cgcggctcgt gagcgcgccc gctcaggccc cgggtggccg gcatgaccgg gcggccggcc
     7681 cggccgcggc cccccatggg cgcggcacac catgatgcgc gagctggccg agctgctggc
     7741 gcgcctgcca caggcccccg cggcgttggt gacggtggtg cgcagcgctg gctcggtgcc
     7801 gcgcgagctc ggcgcctggc tggccgtgtt cggcgatggc gtggtgctgg gcaccatcgg
     7861 cggcgggcac ctggagctgg aggccatcga gcgcgcccgg gcggcgctgg ccgccggccc
     7921 gttcgagccc gaggagcggc gctgcgcgct cggccccagc ctgggccaat gctgtggcgg
     7981 cgtggtgtgg ctgcgccagg agcgcatcga ggcggcgcac atcgaggccc tgcgcacccg
     8041 gctgatgccc gccacccgac cgctggcgct gttcggcggc ggccatgtgg ggcgcgccat
     8101 cgtgcgcctg ctggaggagc tgccgtttgc cgtgcgctgg gtggacagcc gcgacgaggt
     8161 gtttccggcc gtgctgccgc tgaatgtgca ggccgagcat tccgacccgg ttcaggcggc
     8221 ggtggccgag ctgccggcgg gcagcgcggt gctggtgatg agcttcagcc atgccgagga
     8281 cctggaggtc atcgccgcct gtctggcgcg ccagcgcgag cggggtgacc tgagccacat
     8341 cggcctgatc ggcagccgca ccaaatgggc ccgctttggc catcgcctgg ccgaacgcgg
     8401 cttcagccag gccgaactgg cgcgcgtgag ctgcccgatc ggtctgccgg agatccgggg
     8461 caagcagccg gcggtggtgg cgctggcggt ggtggcgcaa ctgctggcgc tggggctggc
     8521 gccgggctga agccaaaaaa atggcgcccg ctgggcgcca tggctgaggg cgggcgaagg
     8581 ccgctggacg ctcagccctg atgcgggcgc ttgcccggat tcttcttgct gcgcgtgtgc
     8641 acgccgcgct ccaggctgcg gcgccaaaaa aatggcgccc gctgggcgcc atggctgagg
     8701 gcgggcgaag gccgctggac gctcagccct gatgcgggcg cttgcccgga ttcttcttgc
     8761 tgcgcgtgtg cacgccgcgc tccaggctgc ggcgctggcc atcggcctgc ttgacgcggg
     8821 gggcgccggg cagcggcggg gtggccttgc gcatggcctc ggtgacgatc ttgttgccca
     8881 tggtgtgatc cttgtgaagt ggtgtgtgga cgaaaaccct atattgtcgc cgattcgcag
     8941 cccatcgtgg caagctcggc acaagggaaa acctgggcgc caggcccacc gaactgcata
     9001 cccaattgta tacaatccga ccgagatcgc agcggtgccc gagagtcgtc catgaggcag
     9061 tgcgtgacgc cccccgatag cgcgatctcc tgaaagcggc ttgcagcgcc cgcggtggca
     9121 agcccgaaga agggacactc acgcatggaa gcatatcttc tcgactgggc caacctgctg
     9181 ctgcgctggc tgcacgtcat caccgccatc gcctggatcg gctcgtcctt ctacttcgtc
     9241 ttcctggaca gcagcctgct ggcgcccacc gccgacgacc tgaaggccaa gggcgtcgac
     9301 ggcgccatct ggtcggtgca cggcgggggc ttttatcacg cgcaaaagta catggtggcg
     9361 cccaagggcg gcatcgacag ccacctgcac tggttctact gggaaagcta ctggacctgg
     9421 atctccggct ttttgctgtt cgtcgtgctg tacctgtgga acgccggcat cttcctggtc
     9481 gacaagcagg tgttcgactg gtcgccgctg gccgccggca tcggcgcgct ggtgttcctg
     9541 gcggcgttct ggttcatcta cgacgccatc tgccgcatct tcggtcagcg tgcgaacggc
     9601 gaccgcatcg tcggcgcgct ggtgctggcg gtggtggtgt gctcgtcgtg gattgcgttc
     9661 cagctgtttt ccggccgtgc ggcgtttctg atggtgggcg cgtcgctggc caccgccatg
     9721 agcgccaacg tgttcttctg gatcatcccc ggccagcgca aggtggtggc ggccatgacc
     9781 agcggccagc cctacaaccc ggccgactac gccatctacg gcaagcgcgg caagcagcgc
     9841 agcgtgcaca acacctactt cacgctgccg gtgctggtgg ccatgctcag caaccactac
     9901 agcttcatct acagccaccc gcagcgctgg gcgattctgc tggtgctgat gctggccggc
     9961 gcgctgattc gcctgttctt cgtgcagcgc cacggctaca agctgggccg cgtgggcaac
    10021 ccctggccct atgcggccgc cggcgtggtg ctgctgctgg cgctgatcgc ctggctcaag
    10081 ccggccccgg cggtgcccgc ggcggccctg ccggccaagg tgagctacgc gcagatccag
    10141 ccgatcctgg cgcagcgctg ctacgtctgc cacggcgagg ccgtgcagca gaagaacctg
    10201 cgcgtggaca gcgaggcgct ggtcagcgcc aacgcccagc tgatctacca gcaggtggtg
    10261 gccagcaagc tgatgccgct ggccaattcg acccagatga ccgaggacga gcgcgccttg
    10321 gtgggccgct ggttccaagg tcagggcaag tgagcccgac gcgccgccgg cgtgggttgc
    10381 cggtggcggg cagacgcgac gctcagccct tcttgctcac catgccgtag atccacagca
    10441 cgatgatggc gccgaccacc gaggcgatcc agccagccgg cgtgccggct gcgtacaggc
    10501 ccagggcggc gccgccgtag ccggccacca gcgagccgac gatgcccagg atggtggtca
    10561 tgatcaggcc catgctctgg ttgcccggca tgatggcgcg cgcgatcagg ccgacgatga
    10621 agccgacgac gagggtgacg atgatgctca tgttggatgc cttccctggt ggatgaacgc
    10681 tggacggcct gcactgtacc gcacccatcg gtcgtccaca cgggcgcgcg gggcgcgcgg
    10741 cggccgccac ggcgcgccgt cggtggcgtg acggttcatg ccaactcgca tgaccaacga
    10801 ccaacgacca atgacggatg acctcgctgc gccgcgaggt ccaaggggtc atgcgtcgcg
    10861 gtttttgaac gccaagccca tcaggttgcg gcggcgcctt gctcgatggc ttcgatctca
    10921 cccgacaggc gcagccactc gtcttcctgc gtctgcagct cctcggccag cgccttcagt
    10981 cgccggccgg cgtcggcgat gtcgccggcg ggcagggggc tggacaggcg ttgctcgagc
    11041 gcgctgcgct cggcgccgag ctgttccatg cgctgctcgg cctgcgccag tgcgcgccgc
    11101 agcggccggg tctgctccgc cagctgctgg cgccgctgcg cctgggcgcg ccgctgctcg
    11161 gccggattga gtggcggttc agcaggtttt ggggccctgg cgctttcctg gcttgcgccg
    11221 tcagctatgg tttcaggagc gcacgcgacg acccgctcgc gcaaccggcg cgcttcatcc
    11281 agcaggtggc gctggtagtc ttccaggtcg ccgtcgaacg gctgcacgct gccgcgcgcc
    11341 accagccaga actcgtcgca cacgctgcgc agcagggcgc ggtcgtggct gaccagcatc
    11401 agcgtgcctt cgaactcgtt gagcgccacg gccagcgcct cgcgcgtggc caggtccagg
    11461 tggttggtgg gctcgtccag cagcagcagg ttggggcgct gccagacgat catggccagc
    11521 accaggcgcg ccttttcgcc gccgctgaac tggcctaccg gctggttgac catgtcgccc
    11581 gggaagttga aggtgcccag gtagttgcgc agttgctgct cgcgcgcctc ttcgctggct
    11641 tcgcgcccgc tctggcgcgc cagccgcacc atgtgctcga gcggcgtgct gtcggtgtgc
    11701 agcacgtcga gttcctgctg ggcgaagtag ccgatggcca ggcccttgcc ctcggtcacg
    11761 ctgccggcca ggggcgccag ctcgcgcgcg atggtcttga ccagggtgga tttgccttgc
    11821 ccgttggcac ccaggatgcc gatgcgctgg ccggccagca cggtgcggct gacgccgcgc
    11881 aggatcacgc ggtcgccggg ccgcaggccg ggcggcagct cgccgcgcgc ggcctgttcg
    11941 gccagcacgg tggcgctggt ggcgtaggcc gggggtgccg cactttcagt gccggaagcg
    12001 ccgcttggac ttgccgggtc tgcgccagca gcttcttttt tgatagcgtc ggcacggtag
    12061 ccgaaagccg cgtcgcggat cgccagcatg gggttgggca ggctgagcgg ggccttgaac
    12121 tcgagctgga actcggcctc ggccagcacc ggggcgatgc gttccatgcg ctccagcgcc
    12181 ttgacgcggc tttgcgcctg gcgcgccttg gtggccttgg ccttgaagcg gtcgatgaac
    12241 ttctgcaggt gggcgatctt gtcctgctgg cgcgcccagg ccgcctgctg ctggctgagc
    12301 tgctcggcgc gcaggtcttc gaaggcgctg tagttgccgc cgtagcgggt caggcgcccg
    12361 ccctcgatgt gcagcgtgac gcgggtgacg gcgtcgagga actcgcggtc gtggctgatg
    12421 acgatcagcg tgccggcata gcgcgccagc cagccttcca gccacaccag cgcgtccagg
    12481 tcgaggtggt tggtgggttc gtcgagcagc agcaggtcgc tgggcgccat cagcgcgcgc
    12541 gccagttgca gtcgcatgcg ccagccgccc gaaaagcggt tgaccggccg cgccagctcg
    12601 tgcggctgaa agcccaggcc caggatcagc gcctcggcgc gcgcgcgggc gtcgtgcgcg
    12661 ccggcgtcgt gcaggtcgct gtgggcctgg gcgatcgcca tgccgtcctg ggtttgctcg
    12721 gctctggaca gctggcggcg cagctcggac aggcgctcgt cgccttgcag cacgaaatcg
    12781 gtggtgctgt cttcggtttc gggcaactgc tgtgccacct gggccaggcg ccagccggcg
    12841 ggcatggcgc agtcgccgct gtcttcgtgc agcgcgccgc tcagcagcgc gaacaggctg
    12901 gacttgccgg cgccgttgcg gccgaccagg ccgacctttt cgcccgggtg cagggtgacg
    12961 ctggcgccgt ccagcagcaa ccgggcgccg cggcgcaggc tgacgttgct gagctggatc
    13021 atggcgccag cgcctcgcgc gtgagcagca gcacctgcgc gtcgccggcg ctggtgtcca
    13081 gccacaccgg ttgcagctgc gggaatgcgg cgtcgaagtg cgcgcgttcg tggccgatct
    13141 ccagcaccag cacgccgccg gcgttcaggt gcgccggcgc ctggcgcagc agcgggcgga
    13201 tgaagtccat gccatcggcg ccgccggcca gcgccagcgc gggctcggcg cgaaactcgg
    13261 gcggcagcgc ggccatgctg gcggcgttga cgtagggcgg gttgcacacg atcaggtcgt
    13321 aggggccggg gcaggcggcc aggccgtcgc tttcgatcag ccggatgcgc tgctgcaggc
    13381 cgtggcgctc gacgttcagg cgcgccaccg ccagcgcatc gcggctcagg tcggccgcat
    13441 ccacctgcac ctcgggccag gccagggcgg ccagcacggc caggctgccg ttgccggtgc
    13501 acaggtcgag cacgcggcgt gtgcgttcgg acagccaggg gtcgatgccg ccgtcggcga
    13561 tcagttcggc gatcaggctg cgcggcacga tggcgcgcgc gtccacatga aacggcacgc
    13621 cttgcagcca ggcctgcccg gtcaggtagg ccagcggctg gcgggtgtcg atgcgcgctt
    13681 tcaaaagcat agctgcttgc gcttgaccgg cgggcgacac gggctgattt ccttcggaac
    13741 cgctgaggtc ggtgtcgagc ggcaggccga gcgcgtgcag caccagccag gcggcttcgt
    13801 cgcgggcgtt ggtggtgccg tggccaaagg ccacgcccgc cgcgctgagc tggcgttcgg
    13861 tgtcggtgat gagttgggtg agtgtggtca tgcggaaaac ggcgccggcg ggtgcgtggc
    13921 cggcgcaccg ctcggggtga aggtcaacgc agctgcgcca cccgggcctg ggcggccagc
    13981 tgctccagca cgcggcggta gatgttcttc agccgctcca gctcggccag ctcgatgtgt
    14041 tcgtcgatct ggtggatgct ggcgttgggc gatcccagct cgatcacctg cgggcagatg
    14101 cgggccagaa agcgcccgtc gctggtgccg ccagtggtgg acaactcggg ctgcaggccg
    14161 gtttcggcgg cgatggcggc ctgcaccgcg tccagcaggc tgcccggcgg ggtgaggaag
    14221 ggctcggccc ccagcgtcca ctcgagcgtg tggtcgagcc cgtggcgttg cagcaccgcc
    14281 tgcagccgcg cctgcaggct ggccggcgtg gaggcggtgc cgtagcggaa gttgaagtcc
    14341 accaccgcct ggcccgggat tacgttgccg gcgccggtgc cggcgtgcag gttgctgacc
    14401 tgccagccgg tgggctgaaa gtgggcgttg ccggcgtccc aggcggtggc ggccagttcg
    14461 gccagggcgg gcgccagctg gtggatcggg ttgcgcgcca agtgcggata ggccacgtgg
    14521 ccctgcacgc cgcgcaccac cagccggccg ctgagcgagc cgcggcggcc gttcttgacc
    14581 atgtcgccgc tgtggcgctg ggcggtcggc tcgccgacga tgcaggcgtg cagccgctcg
    14641 ccgcgcgcgg ccagccactc gcacaccacg cgggtgccat ccagggcgcg gccttcttcg
    14701 tcgctggtca gcagcagggc gagggccagc ggcgcctcgg gccgggcggc gaggaactcc
    14761 tcgagcgcca ccacgaaggc ggcgatggcg cccttcatgt cgctggcgcc acgcccgtac
    14821 agccggcccg cgcgctcggt gggggtgaag ggcgggctgg tccaggcttc gaccgggccg
    14881 gtcggcacca cgtccacatg gccggcaaat actatcgttt caatagcttg cggcgcttgt
    14941 tggtattgcg ctggaacccg tttggaccac agattgcgga cgcgccattc gggcggcccg
    15001 cggtcgatga attcgggctc gaacccgagc ggcgccaggc gttcggcgat caggtcgatg
    15061 cagccgccgt cggccggcgt gacggagggg cgggcgatca gggcttcggc caggcggcgc
    15121 gtggcggtca tggaggggtg aggctggggc gcggcggtga gcaggcccgc attctttcat
    15181 gccccggcgc gcgcggcggg gatgcaggcc ggcatcggcg tgccgccgga cgacccatga
    15241 cgccccgcct tgcccctgac ccatgggtca tcgaagcgcc gcgaggtcat tcgacatgcg
    15301 tcagggatgg acccttaggc atcgccgatg tgtgaatcgg aaaagctgcc gaccttggag
    15361 tcctggaagc ccgaatcgcg gtagccgctg cggctgaacc cggacttgcc gaagccgctg
    15421 gggcgcacgt cgagcgtgat gtcggtgaag gacggttcgt cgctcgggcg ctcttcttct
    15481 tcgggcttgc gcgccaggtg gcggacagtc tggttctgca gtcgccacat caggttggtg
    15541 ggcgagtcgg cgttggccag gccttccttg cggtcgatgc ggttttccag gatcaggcgc
    15601 gccagatcct gctcgaaggt ctgcgagcct tcggcgatgg cgttgtccat ggcgtccttg
    15661 acgccggaga agtcgctctt ctcgatcagg tcggccacca gcttggtgtt gagcagcacc
    15721 tccaccgccg gcacacggct gccttcggtg gtgcgcacca gacgctggga aacgatcgat
    15781 ttgagtgccg ccgacaggtc tcccagcagg gtggggcgca cctcgaccgg gtagaaactg
    15841 agcacgcggt tgagcgcctg gtaactgttg ttggcgtgca gcgtggccag gcacaggtgg
    15901 cccgactgcg cgtaggcgat ggctgcggtc atggtctcgc ggtcgcggat ctcgccgatc
    15961 atgatcacgt cgggcgcctg ccgcagggcg ttcttcagcc cgatctgcag cgccgccgtg
    16021 tcggtgccca cctcgcgctg attgatgagc gacttgtggt tggtgaagac gtattcgatc
    16081 ggatcctcga tggtcaggat gtgaccagtg gcgttgcggt tgcggtggtc cagcatggcc
    16141 gccagggtgg tggacttgcc gttgccggtg gcgcccacca tcagcaccag gccgcgcttt
    16201 tccatcacca ggtgcttgag gatgctgggc aggttgagcg agtccaggtc cgggatctcg
    16261 gggctgatga agcggatcac cgccgcgtag gtgccgcgct ggcgcatggc cgacagacgg
    16321 aaattgccca cgccgtaaat cgggatcgcc atgttcagct ccccggtctc cttcaactcc
    16381 tcgatgcggt tgggcggcac cacgtccatc agcaggttca gcgtcgcctc ggggggcagc
    16441 acctggttgt tgatcggcat gctgacgccg ttgatccgga tggtggccgg tgcgtgggcc
    16501 gacaggtaca cgtccgaggc ctttttttcg gccatcaggt gcaggatgcg gtccatcatg
    16561 gtggtggaag caacaggctt gggcatggca gtgactcgga tgctggacga tggcgcgccg
    16621 gtcgacgcgg gtaggtccat ggcggggcct gccggctcgc gccctgaacc agcctcaata
    16681 ggcaaaacgg tttcattgta ggagcgcaac gaccatcgtg aggccccgac atgccgagaa
    16741 tttcacgctc ggcaccctgt tcaccacaga ccaatcgaca ccagacgttg cgctggtgct
    16801 agtgtctctc gcacaggaac gatccaggaa tgcggcacac cggactactg gaaagtccta
    16861 acaaggaaag cgttgccaag caaggcgttt ccaattgtaa tgtaatcatt tggattgata
    16921 tcctgactgt ctgaacatga tttacgccaa atccgaatct ggcctgcagg cggtcaagga
    16981 tcgccatgcg gtggacctga cccgagcgca gcgctcggtg ctgatattga ttgacggcaa
    17041 acgcagcgtg cgcgaagtgc tggaggccac ggcgacgctg ggtgtgacgg cgacagacgt
    17101 cgagctgctg gtgcaacgcg ggctggtggt ggtggccagc ggcggcatgt cgctcaacgg
    17161 tagcgcgggc ggtgcctcgc tgaacgggtc gtccggcgga gccgggcgcg tgacggcgac
    17221 cggtgatggc gccgcttccg ggtcgggtga tcctgcggca cccgatctga cggacgacga
    17281 acgcgctcgg cgctaccgtg tggcctatcc catggccacc cagctgacgg ccaaactggg
    17341 actcaagggc ttccggttga acctggcggt ggaggaagca gctggctttg agggtctggt
    17401 ggacctgttg ccgcgcctgc gcagtgccct gggcagcgag ggtgtgcgtc ctctggagag
    17461 cgtgctgatc ggtgatccgc tgcagtgacc cgggagatgc ggcatcgtgg gttcgatcgg
    17521 gtgcccggtg tgccctcgcc aacatcgagc agaagcgacc tccagcgttt gctgcaaaag
    17581 cgcacgcagc tatcaatagc atagtgcatc ggatgatcgg cgagcgatcg taggcgcact
    17641 tgtcgcaggt cgcgcgcgct atcattgaca gtttccgcat ttcaccgtcg gcccgcgggc
    17701 ctgtccagcg ccagtgcgtc tcaattccat caagctgtcg ggcttcaagt ccttcgccga
    17761 acccaccaac ttc
//
LOCUS       DAOOOG010000034        17105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_3488_length_17105_cov_25.269736, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000034 DAOOOG010000000
VERSION     DAOOOG010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 17105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17105
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3488_length_17105_cov_25.269736"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PK242_04450"
     CDS             complement(<1..759)
                     /locus_tag="PK242_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOZ93300.1"
                     /translation="MSAAPTPGTLYLVPTPLDHGCPLQAPLTDALPQGTIEVAARLSH
                     WISENARSTRAFLKRVAALVPLAQPLQAQHIQQLPHAIHKQGDHRRAGTWDARPLLAS
                     ALQGHDLGLVSEAGMPAVADPGSSVVRAAHDLGIEVRPLVGPVSMLLALAASGLNGQS
                     FAFVGYLPQEASRRATRLRELEAWAQQSGQSQLFIETPYRNAALLQALLQQLRPDTRL
                     AVCSGLTLAQQRCTSLRVADWRLRSAALTADLSAQ"
     gene            complement(756..1364)
                     /locus_tag="PK242_04455"
     CDS             complement(756..1364)
                     /locus_tag="PK242_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOZ93301.1"
                     /translation="MPTDTTDASAAPPLVLGSTSPYRRELLSRLRLPFTTAAPQVDET
                     PLPHETPAALARRLALAKAREVAARHPQAIVIGADQVADLAGEPLGKPGSHERAVAQL
                     QRMSGQTVVFQTALSVVCLASGFEQSELAPVEVRFRALDAGEIERYLRAEQPYDCAGS
                     AKSEGLGIALLEAIHNDDPTALVGLPLIRTARLLRAAGLRLP"
     gene            1391..1942
                     /locus_tag="PK242_04460"
     CDS             1391..1942
                     /locus_tag="PK242_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOZ93302.1"
                     /translation="MSRSFDARRLDVAALAEAGASLSGADELQNYERLALEAQGADKD
                     LTVEWKVQGGRRQAVDGQWRPSLHLQAQTRLPLTCQRCLGPVATPLVVDRHLVFVESE
                     ARAAALDDASDDDVLALTPALDLRELIEDELLLALPLVPRHEVCPDAVRLSAHSEGFD
                     AAPPQRPHPFAALAALKQGGKGR"
     gene            2100..2282
                     /gene="rpmF"
                     /locus_tag="PK242_04465"
     CDS             2100..2282
                     /gene="rpmF"
                     /locus_tag="PK242_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOZ93303.1"
                     /translation="MAVQQNKKSPSKRGMHRSHNALSVPGIAVEPTTGETHLRHHISP
                     NGFYRGRQVIKSKTEA"
     gene            2468..3505
                     /gene="plsX"
                     /locus_tag="PK242_04470"
     CDS             2468..3505
                     /gene="plsX"
                     /locus_tag="PK242_04470"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOZ93304.1"
                     /translation="MIVLAVDCMGGDHGPAVTLPACRQFLDRHPEAELLLVGQPEALA
                     GLTHERARVIAASEVVTMDDAVEVALRRKKDSSMRVAIQQVKDGAAQAAVSAGNTGAL
                     MAVARYLLKTLEGIDRPAIATQIPNQKGGATTVLDLGANVDCTAEHLLQFAVMGSALV
                     SVLNDDPQPTVGLLNIGEEAIKGSEEIKRAGELLRAAGDAGDLNFVGNVEGNDIFKGT
                     ADIVVCDGFVGNVALKTIEGLASMIGGFMKEEFSRNLLTKAAAVVAYPVLAALKRRMD
                     HRRYNGAALLGLRGLVFKSHGSADALAFEQALVRAYDAASHQLLARVQQRIAHAAPLL
                     AASGAAAAPAA"
     gene            3547..4527
                     /locus_tag="PK242_04475"
     CDS             3547..4527
                     /locus_tag="PK242_04475"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOZ93305.1"
                     /translation="MSRRYSRITGTGSYLPPRRVTNADLVAELASRGIETSDEWIVER
                     TGIRARHLAAPDVPASELAVPAARQALAAAGLQPGDIDLIIVATSTPDMVFPSTAAIV
                     QHKLGMAGCPVFDVQAVCSGFVYALTVADALIANGTARRALVIGAEVFSRILDWSDRT
                     TCVLFGDGAGAVVLEASDRPGILATDLHADGKYVDILCVPGGVSGGQVLGDPLLKMDG
                     QAVFKLAVGVLDKAARAVLAKAGKSEADIDWLIPHQANLRIMHGTARKLHLPLDKVIV
                     TVNEHGNTSAASIPLALDHGVRSGQIQPGQTLLLEGVGGGFTWGAVLLNM"
     gene            4588..5538
                     /gene="fabD"
                     /locus_tag="PK242_04480"
     CDS             4588..5538
                     /gene="fabD"
                     /locus_tag="PK242_04480"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOZ93306.1"
                     /translation="MKPFAFVFPGQGSQAVGMLDAWGDHPAVRDTLAEASAALGEDLG
                     ALIAQGPKEALALTTNTQPVMLVAGVASWRAWRAEGGALPAAVAGHSLGEYSALVAAE
                     VLTLAQAVPLVRLRAAAMQQAVPVGTGAMAAILGLDAQKVIAGCAEATAAMGQAGAEV
                     VEAVNFNDPAQTVIAGSKAAVDKACEVLKGLGAKRALPLPVSAPFHSSLMKPAAEQLK
                     AALAALELGAPRIPVVNNIDVAVEQEADRIRDALYRQAFGPVRWVECVQKIRALGIDT
                     VVECGPGKVLAGMTKRIDAGLTGAALYDPATLAEVKALLG"
     gene            5566..6315
                     /gene="fabG"
                     /locus_tag="PK242_04485"
     CDS             5566..6315
                     /gene="fabG"
                     /locus_tag="PK242_04485"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOZ93307.1"
                     /translation="MADTPFAGQVALVTGASRGIGAAIAAQLAERGLKVIGTATTEDG
                     AARIGAALAAHPGCRGAALDVNDAAAAEALIEAIVKEHGALHVLVNNAGITRDMLAMR
                     LKDEDWDAVLDTNLKAVFRMSRAVMRPMMKQRYGRIVNITSVVGASGNPGQANYAAAK
                     AGVAGMTRALARELGSRGITVNCVAPGFIATDMTAALGEEQHKALAAQIPLGRLGQPA
                     DIAAAVAYLASPEAGYVTGQELHVNGGMFMA"
     gene            6418..6654
                     /gene="acpP"
                     /locus_tag="PK242_04490"
     CDS             6418..6654
                     /gene="acpP"
                     /locus_tag="PK242_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOZ93308.1"
                     /translation="MSDIEARVKKIIAEQLGVEESQVTNEKAFVADLGADSLDTVELV
                     MALEDEFGIEIPDEDAEKITTVQAAIDYAVSKKA"
     gene            6818..8062
                     /gene="fabF"
                     /locus_tag="PK242_04495"
     CDS             6818..8062
                     /gene="fabF"
                     /locus_tag="PK242_04495"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOZ93309.1"
                     /translation="MNRRRVVVTGLGCVSPVGNTVAESWASLLAGRSGIDLITRFDAS
                     AFACRIAGEVRGLDISPHISAKEARTMDTFIHYGIVAAAEAVQDAGLATGEALDDEAA
                     TRIGCVVGSGIGGLPLIEETQTEYAARGPRRISPFFVPASIVNMVAGHVSMKFGFKGP
                     NLAIATACTTGLHCIGEAGRLIEYGDADVMIAGGAESTVSPLGVGGFAAMRALSTRND
                     DPKTASRPWDKDRDGFVLGEGAGILVLEAYEHAKARGARIYAELAGFGMSADAGHMTA
                     PNMDGPRRAMVAALRNAGLNADQVQYVNAHGTSTPLGDINESNAIKAALGDAARRAVV
                     SSTKSMTGHLLGGAGGIESVFSVLALHHQKVPPTINLFNQDPECDLDYCANSARDMKI
                     DAVLKNNFGFGGTNGSLVFRRL"
     gene            8148..8606
                     /locus_tag="PK242_04500"
     CDS             8148..8606
                     /locus_tag="PK242_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93310.1"
                     /translation="MSYPVGRSAALARVLLGLAAAGWLALGLTYFLAPELIQLAPAAM
                     LSIAINALVTLGLWRFWRAQEARQLRWTGERWLLHTPGTEPDRGGEGAGVEVRLDAQR
                     WLLLRFRDPAEAGAHWLWAQASHDPARWHLLRCALYSAAPSPAATQASRP"
     gene            8653..9273
                     /gene="rpoE"
                     /locus_tag="PK242_04505"
     CDS             8653..9273
                     /gene="rpoE"
                     /locus_tag="PK242_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HOZ93311.1"
                     /translation="MAPPPAPADSDALLVERAAAGDTRAYELLVIKYQRRIERLIGRL
                     VRDVDLVPDIAQETFLRAWRALPQFRGEAQFYTWLYRIAVNTAKKSLLELKRDPVLTE
                     SALHTSDDDDETSRRENELISESTPESELAAREIAAAVNAAVEALPEDLRQAITLREI
                     EGLSYEEIAHTMNCPIGTVRSRIFRAREAISARVKPLLSHQTGKRW"
     gene            9302..9952
                     /locus_tag="PK242_04510"
     CDS             9302..9952
                     /locus_tag="PK242_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HOZ93312.1"
                     /translation="MNARIDQTLDLEAISALMDGQLRDDDAARALGMMTASGQARESW
                     QLYHLVGDVLRSADLAACGQDRAFAARLAATLAAQRPAEVAAPRAPALSPHALTRPAA
                     NDGLFGWRMVAGLASFAAVAVIGWGMLGDLGPQRQGGAQLAQGAAAPRTALQTVAGPS
                     PALSTAEPDEIRVADGPAVMLRDPRLDELLQAHRQVSATPALGGFVLNATYEGAGR"
     gene            10021..11007
                     /locus_tag="PK242_04515"
     CDS             10021..11007
                     /locus_tag="PK242_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HOZ93313.1"
                     /translation="MVSLAGAQSIGQFGGGAPAGVVGPSQRSVAEWLARLQQGARVPS
                     YIGTFVVSSASGAMSSSRIWHVCEGDVQLERVDALTGVPRSTLRRNDTVMTFLPDARV
                     VTLDQRQLGAVFPNLLNLAQGLPVADYYDARELGRGRVAGCDAEIVQLLPRDEWRYGY
                     RIWSERRTGLVLKTQTLNAHGRVLEQAAFSEVQLDAPVQAEQLRQMMANTQGYRIVKA
                     EHARTTPQAEGWQLTTMVPGFEARNCYRRPPSASASLVQCLFSDGLATVSLFMQPLDP
                     ARHQQEGAVAMGATQTLTRRVVDRSGQWWVTAVGEVPPATLQAFVRGLERIP"
     gene            11064..12539
                     /locus_tag="PK242_04520"
     CDS             11064..12539
                     /locus_tag="PK242_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOZ93314.1"
                     /translation="MNKLSLAALAVPMLLSATLNLPLAAQAQAPAAPIVRGLPDFTDL
                     VEQVGPAVVNIRTVERVRTGIGRNGPMMDEEMQEFFRRFFGLPLPGVPRQGPRQQEPQ
                     EPAEREVPRGVGSGFILTADGFVMTNAHVVDGADEVLVTLTDKREFKARIVGADKRTD
                     VAVVKIEASGLPAVRIGDVSRLKVGEWVMAIGSPFGLDNTVTAGIVSAKQRDTGDYLP
                     FIQTDVAVNPGNSGGPLINMRGEVVGINSQIYSRSGGFMGISFAIPIDEAMRVSEQLR
                     ASGRVTRGRIGVQIDQVSKEVAESIGLGKPQGALVRGVESGSPADKAGVEAGDIITRF
                     DGRVIDKASDLPRLVGNTKPGTRSALTVFRRGQTRELNITIAELEPEQTARRPSQRGS
                     APPKASEATQVLGLTVTELTEAQKKELKVRHGVRVEAATDAGARAGLREGDVILSVGN
                     AEVRSVRDFEAAVAKAERGKPISMLVQRGELVQFVLVRPGR"
     gene            13016..14824
                     /gene="lepA"
                     /locus_tag="PK242_04525"
     CDS             13016..14824
                     /gene="lepA"
                     /locus_tag="PK242_04525"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOZ93315.1"
                     /translation="MDHIRNFSIIAHIDHGKSTLADRLISRCGGLSDREMSEQVLDSM
                     DIEKERGITIKAQTAALHYQARDGRVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTALELGVEVLPVLNKMDLPQADPDNAKAEIEDVIGIDASEAIA
                     ISAKTGMGIDDVLEQIVAKVPPPRGQAQAPLRAMIIDSWFDAYVGVVMLVRVVDGRLA
                     KGERFKMMASGSAYEANQLGVFTPAQQAREVLEAGEVGYIIAGIKELKAAKVGDTITL
                     EKKLPGNLGPADRPLPGFKEIQPQVFAGLYPTEANQYDQLRDALEKLQLNDASLHFEP
                     EVSQALGFGFRCGFLGLLHMEIVQERLEREFDQDLITTAPSVVYEVEKADGEVIQVEN
                     PSKMPDQGKIHEIREPIVTVHLYMPQDYVGPVMTLANQKRGVQINMQYHGRQVMLTYE
                     LPLGEIVLDFFDKLKSVSRGYASMDYEFKEYRPSDVVKVDILLNGEKVDALSIIVHRS
                     QAQFRGRAVAAKMREIISRQQFDVAIQAAIGANIIARENIKALRKNVLAKCYGGDITR
                     KRKLLEKQKAGKKRMKQIGSVEVPQEAFLAILQVED"
     gene            14824..15792
                     /gene="lepB"
                     /locus_tag="PK242_04530"
     CDS             14824..15792
                     /gene="lepB"
                     /locus_tag="PK242_04530"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOZ93316.1"
                     /translation="MPLLTAVILAAFVAYAAAWYVGILQGNFALLLLLATLVTGAYWL
                     GERFVFLPRRRAAAQALEAQAAQRRSELAAQGITQVDEGELTQARERVLAQPWWLDWT
                     AGLFPVIAVVFLLRSFVFEPFKIPSGSMVPTLLVGDLILVNKYTYGLKLPVIHTRLTQ
                     GQPVQRGDVVVFRYPPQPSVDYIKRVVGLPGDEVAYLNKQLTINGQPVPQTAVSDFFD
                     EQAMEYFKQFDERLGVTNHRIIVDGRRPAFVGGASDFEYKDNCRYSVEGVRCKVPPGH
                     YFMMGDNRDNSLDSRFWGFVPDGHIVGKAFFVWMNFGNLKRIGSFH"
     gene            15818..16186
                     /locus_tag="PK242_04535"
     CDS             15818..16186
                     /locus_tag="PK242_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HOZ93317.1"
                     /translation="MKHQGPSSQRGISFFGLLFLGVVLALLAIVGARVVPTASEFMTI
                     QKAVKKAAADGDTVPAVRASFDRTASVDYITSISGKDLEVTKQGDKVVVNFAYDKEIP
                     LFGPAYLLIKYRGSSAGGYD"
     gene            16245..16904
                     /gene="rnc"
                     /locus_tag="PK242_04540"
     CDS             16245..16904
                     /gene="rnc"
                     /locus_tag="PK242_04540"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOZ93318.1"
                     /translation="MQERLQYHFRDESLLERALTHRSFSADHNERLEFLGDSVLGLGV
                     AHMLYQALGGGAEGDLSRLRAQLVRQDSLHQLALHLGLPPLLRLGEGELRSGGQRRPS
                     ILADALEALIGAVYLDGGHAPADALVRRLFEQVEISPAMSAAAKDAKTALQEWLQARK
                     MRLPQYEVVRVLGAAHRQTFEVRCQVAEQNLEVLGQGASRRAAEQCAAASMLEQLQNR
                     P"
     gene            16901..>17105
                     /locus_tag="PK242_04545"
     CDS             16901..>17105
                     /locus_tag="PK242_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOZ93319.1"
                     /translation="MNTPEIIADGADPERAGARSGTPSAPDPGASVQRCGLIAIVGKP
                     NVGKSTLLNALVGQKISITSRKAQ"
BASE COUNT         2704 a         5770 c         5950 g         2681 t
ORIGIN      
        1 ctgagccgac aggtctgccg tcagtgccgc tgaccgtagg cgccagtcgg ccacgcgcag
       61 gctggtgcag cgttgctgcg ccagcgtcag cccgctgcac accgccagcc gggtgtcggg
      121 tcggagctgc tgcagcaggg cctgcagcag ggctgcgttg cggtagggcg tttcgatgaa
      181 caactggctt tggccgcttt gctgtgccca ggcctccagt tcgcgcaggc gcgtggcgcg
      241 gcggctggcc tcctgcggca gatagcccac gaacgcgaag ctctggccgt tcagcccgct
      301 ggcggccagc gccagcagca tcgacaccgg accgaccagg ggccgcacct cgatgcccag
      361 atcgtgggcg gcgcgcacca ccgaactgcc agggtcggcc accgcgggca tgccggcttc
      421 gctgaccaag cccaggtcgt ggccctgtag ggccgaggcc agcagcgggc gggcgtccca
      481 ggtgccggcg cgccggtgat cgccctgttt gtggatggcg tgcggcagct gctggatgtg
      541 ctgcgcctgc agcggttgcg ccagcggcac cagcgcggcg acgcgcttga gaaaggcgcg
      601 ggtcgatctg gcgttctcgc tgatccagtg cgacaggcgc gcggcgacct cgatcgtgcc
      661 ctggggcagg gcgtcggtca ggggcgcctg gagggggcag ccatggtcca gcggcgttgg
      721 caccaggtac agcgtgccgg gggtcggggc cgcgctcatg gcagccgcag cccggcggcg
      781 cgcagcagcc gggcggtgcg gatcagcggc aggccgacca gcgccgtggg gtcgtcgttg
      841 tggatggcct ccagcagggc gatgcccagg ccttcgctct tggcgctgcc ggcgcagtcg
      901 tagggttgtt cggcgcgcag gtaacgctcg atctcgcccg cgtccagcgc gcgaaagcgc
      961 acctcgaccg gcgccagctc gctctgctcg aagccgctgg ccaggcacac caccgacagg
     1021 gcggtctgaa acaccacggt ctgcccgctc atgcgctgca actgggccac ggcgcgttcg
     1081 tggctgccgg gcttgcccag cggctcgccg gccaggtcgg ccacctggtc ggcaccgatg
     1141 acgatggcct gcgggtggcg ggccgccact tcgcgcgcct tggccagggc cagccggcgc
     1201 gccagcgccg ccggtgtttc gtgggggagc ggggtttcgt ccacctgcgg cgcagcggtg
     1261 gtgaacggca ggcgcaggcg cgacagcagt tcgcggcgat agggggaggt ggagcccagc
     1321 accagcggtg gcgcggcgct ggcgtcggtg gtgtcggtcg gcatggcgcc gattctctta
     1381 cactggccgc atgagcagga gctttgatgc gcggcgcctc gacgtggcgg ctttggccga
     1441 ggccggggcc agcctttcgg gcgccgatga gctacaaaat tatgagcgcc tggcgctcga
     1501 agcgcaaggc gcggacaagg atctgacggt cgaatggaag gtccagggcg ggcgccgtca
     1561 ggcggtcgat ggccaatggc ggccgtcgct gcatctgcag gcgcagacac gcttgccgct
     1621 gacctgtcag cgctgtctgg gtccggtggc gacgccgctg gtggtggacc ggcacttggt
     1681 atttgttgaa agcgaggcgc gcgccgccgc tctggacgac gcgtccgacg acgacgtgct
     1741 ggcgctgaca ccggcgctcg atctgcgcga gctgatcgag gacgagctgc tgctggcgct
     1801 gccgctggtg ccgcgtcacg aggtctgtcc ggacgccgtg cgtctgtcgg cgcacagcga
     1861 ggggttcgac gccgcaccgc cgcagcggcc ccatcccttt gctgcgctgg cggcgctgaa
     1921 gcagggcggc aagggccgat agtgcgtgcc gctgtggcgc gcccacgctt tcgcgctata
     1981 atttaaggct tcgcattcgg cggggcgcgg ccattggctg ccccaggcca ggatgcatcg
     2041 cttacccact tgcccgcgtc cacgcccgag cggcggggcc catccaacaa ggagccacca
     2101 tggctgttca gcaaaacaag aagtcgccgt ccaagcgcgg tatgcaccgt tcgcacaacg
     2161 cgttgtccgt gcccggcatc gctgtggaac cgaccaccgg cgaaacccac ctgcgccacc
     2221 acatcagccc caacggcttc taccgcggtc gccaggtcat caagtccaag accgaagcct
     2281 gatcgatggc gcgggcgcgc ggcgtgcgtg cgaccgtcgc cgttttccat ccgccgatga
     2341 ctccctggcc cggcgctacg acccgtagcc tcgggccttc ggcgtttgcg cgtccgggcg
     2401 atccagcccg gttcatggtt cgagccgtgc cgtcgcgcga cggcggcggc gctgttgcgg
     2461 ggcttgcatg atcgttctgg ccgtcgattg catggggggt gaccacggcc ccgcggtcac
     2521 cttgccggcg tgccggcagt ttctcgaccg ccaccccgag gccgagctgc tgctggtcgg
     2581 ccagccggag gcgctggcgg gcctgacgca cgagcgcgcc cgggtgatcg ccgccagcga
     2641 agtggtgacc atggacgacg cggtggaggt ggccctgcgt cgcaagaagg attcgtccat
     2701 gcgcgtggcc atccagcagg tcaaggacgg cgcggcccag gcggcggtgt cggccggcaa
     2761 caccggcgcg ctgatggcgg tggcgcgcta cctgctcaag acgctggagg gcatcgaccg
     2821 cccggccatc gccacccaga ttcccaacca gaagggtggc gccaccaccg tgctcgacct
     2881 gggcgccaac gtggactgca cggccgagca cctgctgcag ttcgccgtca tgggctcggc
     2941 cctggtgtcg gtgctcaacg acgacccgca gcccacggtc ggcctgctca acatcggtga
     3001 agaggccatc aagggcagcg aggagatcaa gcgcgccggc gaactgctgc gcgctgcggg
     3061 cgatgccggc gacctgaact tcgtcggcaa cgtcgagggc aacgacatct tcaagggcac
     3121 ggccgacatc gtggtgtgcg acggcttcgt tggcaacgtg gcgctcaaga ccatcgaggg
     3181 gctggccagc atgatcggcg gcttcatgaa ggaagagttc tcgcgcaacc tgctgaccaa
     3241 ggcggcggcc gtcgtcgcct atccggtgct ggccgcgctc aagcgccgca tggaccaccg
     3301 gcgctacaac ggcgccgcgc tgctggggct gcgcggtctg gtgttcaaga gccatggttc
     3361 ggccgacgcg ctggccttcg agcaggcgct ggtgcgcgcg tatgatgccg ccagccatca
     3421 attgctggcg cgcgtgcagc agcgcatcgc ccatgcggcg ccgcttctgg cggcgtccgg
     3481 ggcggctgca gcgccggctg cctgagcccg gcgcggccgc ggcccggcat ccacacaagc
     3541 atgttcatga gcagacgcta ttcccgcatc accggcaccg gcagctacct gccgccgcgc
     3601 cgcgtcacca acgcggatct ggtggccgag ctggccagcc gcggcatcga gacttcggac
     3661 gaatggatcg tcgagcgcac cggcatccgt gcgcgccatc tggcggcgcc cgacgtgccc
     3721 gccagcgagc tggcggtgcc cgcggcgcgg caggcgctgg cggcggcggg gctgcagccg
     3781 ggagacatcg acctcatcat cgtcgccacc tcgacgcccg acatggtgtt cccgtccacc
     3841 gccgccatcg tgcagcacaa gctgggcatg gccggctgcc cggtgttcga cgtgcaggcg
     3901 gtgtgcagcg gcttcgtcta cgcgctcacg gtggccgatg cgctgatcgc caatggcacg
     3961 gcgcgccgcg cgctggtgat cggcgccgag gtgttttcgc gcatcctcga ctggagcgac
     4021 cgcaccacct gcgtgctgtt cggtgacggc gccggcgccg tggtgctgga agccagcgac
     4081 cggcccggca tcctggccac cgacctgcac gccgatggca agtacgtcga catcctgtgc
     4141 gtgcccggtg gcgtctcggg cgggcaggtg ctgggcgatc cgctgctcaa gatggacggc
     4201 caggcggtgt tcaagctggc cgtcggcgtg ctcgacaagg ccgcccgcgc cgtgctggcc
     4261 aaggccggca agagcgaggc cgacatcgac tggctgatcc cgcaccaggc caacctgcgc
     4321 atcatgcacg gcaccgccag aaagctgcac ctgccgctgg acaaggtgat cgtcaccgtg
     4381 aacgagcacg gcaacacctc ggcggcgtcg atcccgctgg cgcttgacca cggcgtgcgc
     4441 agcgggcaga ttcagcccgg ccagacgctg ctgctggaag gcgtgggcgg tggcttcacc
     4501 tggggtgcgg tgctcctcaa tatgtagctg ctggcgcttg ctggataagc gctggagtgt
     4561 tttttcttca tttttcctga gcccatcatg aaaccctttg ctttcgtctt tcctggccag
     4621 ggctcgcagg ccgtgggcat gctggatgcc tggggcgacc acccggcggt gcgcgacacg
     4681 ctggccgagg cctcggccgc gctgggcgaa gacctgggcg cactgatcgc ccagggtccc
     4741 aaggaggcgc tggcgctcac caccaacacc cagccggtga tgctggtggc cggcgtggcc
     4801 agctggcgcg cctggcgcgc cgaaggtggc gcgctgccgg cggcggtggc cgggcattcg
     4861 ctgggcgagt actcggccct ggtggccgcc gaagttctga cgctggccca ggccgtgccg
     4921 ctggtgcgcc tgcgcgccgc cgccatgcag caggcggtgc cggtgggcac gggcgccatg
     4981 gccgccattc tggggctgga tgctcaaaaa gtgatcgccg gctgcgccga agccacggcc
     5041 gccatgggcc aggcgggcgc cgaagtggtg gaggcggtca atttcaatga cccggcgcag
     5101 accgtgatcg ccggcagcaa ggcggcggtc gacaaggcct gcgaggtgct caagggcctg
     5161 ggcgccaagc gcgcgctgcc actgccggtg tcggcgccgt ttcactccag cctgatgaag
     5221 ccggccgccg agcagctgaa ggcggcgctg gcggcgctcg aattgggggc gccgcgcatc
     5281 ccggtggtca acaacatcga cgtcgcggtc gaacaggagg cggaccgcat ccgcgacgcg
     5341 ctctaccgcc aggccttcgg gccggtgcgc tgggtcgagt gcgtgcagaa gatcagggcg
     5401 ctgggcatcg acaccgtggt cgaatgcggc cccggcaagg tgctggccgg catgaccaag
     5461 cgcatcgacg ccggtctgac cggcgccgcc ctgtacgacc cggccacgct ggccgaagtc
     5521 aaggcgctgc tgggctgagc cgcacgccac gaggagaccc atcacatggc agacacccca
     5581 ttcgcaggcc aggtggccct ggtcacgggc gcctcgcgcg gcattggcgc ggccatcgcg
     5641 gcgcaactgg ccgagcgcgg cctcaaggtg attggcaccg ccaccaccga agacggggcg
     5701 gcgcgcatcg gcgctgcgct ggcggcgcac cccggctgcc gcggcgccgc gctcgacgtc
     5761 aacgacgccg ccgcggccga agcgctgatc gaggccatcg tcaaggaaca cggcgcgttg
     5821 cacgtgctgg tcaacaacgc cggcatcacg cgcgacatgc tggccatgcg gctgaaggac
     5881 gaggactggg acgcggtgct ggacaccaac ctgaaggcgg tgtttcgcat gagccgcgcc
     5941 gtcatgcggc ccatgatgaa gcagcgctat ggccgcatcg tcaacatcac cagcgtggtc
     6001 ggcgcttcgg gcaacccggg gcaggccaac tacgccgccg ccaaggccgg cgtggccggc
     6061 atgacgcgcg cgctggcgcg cgagctgggc agccgcggca tcaccgtcaa ctgcgtggcg
     6121 cccggtttca tcgccaccga catgaccgcc gccctgggcg aggaacagca caaggcgctg
     6181 gcggcgcaga ttccgctcgg ccgcctgggt cagccggccg acattgccgc cgccgtggcc
     6241 tatctggcca gccccgaggc gggctacgtc acaggtcagg agctgcacgt caacggcggc
     6301 atgttcatgg cctgagccat taccatcggc cactggccgt cgcctgtcgc gtctgctccg
     6361 gtctgcgggg cggcgcggct agaatccacc atttcttact agccccagag ggaacccatg
     6421 agcgatatcg aagcacgtgt caagaaaatc atcgccgagc agctcggcgt ggaggagtca
     6481 caggtcacca acgaaaaagc cttcgtggcc gacctgggcg ccgattcgct cgacacggtt
     6541 gaactggtga tggcgctgga agacgagttc ggcatcgaaa tccccgacga ggacgccgaa
     6601 aagatcacca ccgtgcaggc ggccatcgac tacgccgtca gcaagaaggc gtaaccccca
     6661 ccgcggtttt gccgccggcg tgggcgcgac ccccagcggc cggcccgcgc cacgcagcgc
     6721 ttcgtggccg agcgcgtgcg gtcggacggt ctgagccgtc ttgcacgcac cgtccggcgg
     6781 agcacgacca cagacagaca agcaagatcg gatgaccatg aatcgacgcc gtgttgtcgt
     6841 caccggcctg ggttgcgtca gccccgtggg caacacggtg gccgaaagct gggccagcct
     6901 gctggccggc cgctcgggca tcgatctcat cacgcgcttc gacgccagcg ccttcgcctg
     6961 ccgcatcgcc ggcgaagtgc gcgggctgga catttcgccg cacatctcgg cgaaggaagc
     7021 gcgcaccatg gacaccttca tccactacgg catcgtggcc gccgccgagg cggtgcagga
     7081 cgccggcctg gccacgggcg aggcgctgga cgacgaggcc gccacgcgca tcggctgcgt
     7141 ggttggctcg ggcatcggcg gcctgccgct gatcgaagag acccagaccg agtacgccgc
     7201 ccgcggtccg cgccgcattt cgccgttttt cgtcccagcg tccatcgtca acatggtggc
     7261 tggtcatgtg tcgatgaaat tcggcttcaa ggggcccaac ctggcgatcg ccaccgcctg
     7321 caccaccggc ctgcactgca tcggcgaggc cgggcgcctg atcgaatacg gcgacgccga
     7381 tgtgatgatt gccggtggcg ccgaatccac ggtgtcgccg ctgggcgtgg gtggttttgc
     7441 cgccatgcgc gcgctgtcca cccgcaacga cgaccccaag accgcctcgc gcccctggga
     7501 caaggaccgc gacggcttcg tgctgggcga gggtgccggc atcctggtgc tcgaggcgta
     7561 cgaacacgcc aaggcgcgcg gcgcccgcat ctatgccgaa ctggcgggct tcggcatgag
     7621 cgccgacgcc ggccacatga ccgcccccaa catggacggc ccgcgccgcg ccatggtggc
     7681 tgccctgcgc aacgccggcc tgaacgccga ccaggtgcag tacgtcaacg cccacggcac
     7741 ctccaccccg ctgggcgaca tcaacgaatc caacgccatc aaggcggcgc tgggcgatgc
     7801 cgcccggcgc gcggtggtca gctccaccaa gtccatgacc ggccacctgc tgggcggcgc
     7861 cggcggcatc gaaagcgtgt tcagcgtgct ggccctgcac caccagaagg tgccgcccac
     7921 catcaatctt ttcaaccagg atcccgagtg cgacctggac tactgcgcca acagcgcgcg
     7981 cgacatgaag atcgacgcgg tgctgaagaa caacttcggc tttggcggca ccaacggttc
     8041 gctggtgttc aggcgccttt gattgcgctg cagcgcgcgc tgggcgacgc ccgacgtctc
     8101 cgcccggcgg cgcctgaccg acagcccacg tggcccacgc ccctgccgtg agttatccgg
     8161 tcggccgcag cgccgcgctg gcgcgcgtgc tgctgggcct ggcggcggcc ggttggttgg
     8221 ccctgggttt gacatatttc ttggctccag agcttataca gcttgcgcca gcagctatgc
     8281 taagcatagc aatcaatgcg ctggtgacgc ttgggctgtg gcgcttctgg cgcgcgcagg
     8341 aggccaggca actgcgctgg accggcgaac gctggctgct gcacaccccg ggcaccgagc
     8401 cggaccgggg cggcgagggc gccggtgtcg aggtgcggct ggacgcccag cgctggctgc
     8461 tgctgcgctt tcgcgacccg gccgaagcgg gtgcgcactg gctgtgggcc caggcttccc
     8521 acgatccggc gcgctggcac ctgctgcgct gtgcgctata ttcggccgcg ccttcgcctg
     8581 ccgcgaccca ggcttcgcgc ccctgaccac cggcgcgccg cctgtgctgg acccctgttc
     8641 gtgaccgatt ccatcgcccc cccgcctgcg cccgccgaca gcgatgcgct gctggtcgag
     8701 cgcgcggcgg caggcgacac gcgcgcctat gagctgctgg tcatcaagta ccagcgccgc
     8761 atcgagcgcc tgatcgggcg cctggtgcgc gacgtcgatc tggtgcccga catcgcgcag
     8821 gaaacctttc tgcgcgcctg gcgggcgctg ccgcagtttc gcggggaagc ccagttctac
     8881 acctggctgt accgcatcgc ggtcaacacg gccaagaagt cgctgctgga gctcaagcgc
     8941 gatccggtgc tgaccgagtc ggccctgcac accagcgacg acgacgatga aacttcccgg
     9001 cgggagaacg aactaatctc cgagtccacg ccggagagcg aactggcggc acgtgaaatc
     9061 gccgcagccg tcaatgccgc cgtcgaggcc ctgcccgagg atctgcgcca ggccatcacg
     9121 ctgcgcgaga tcgagggcct gagctatgaa gagattgcac acaccatgaa ctgcccgatc
     9181 ggtaccgtcc gctcgcgcat cttccgcgca cgcgaagcga tttcggctcg ggtcaagccc
     9241 ctgctgagtc accagaccgg caagcgctgg tgacggcaac ccctgcaatc ggtgatcacc
     9301 catgaacgct cgaatcgatc aaacgctgga tctggaagcc atctcggccc tgatggacgg
     9361 gcagctgcgc gatgacgacg cagcccgggc cctcggcatg atgaccgcct ccggccaggc
     9421 gcgtgaatcc tggcagctgt atcacctggt gggcgatgtg ctgcgctcgg ccgatctggc
     9481 cgcctgcggg caggatcggg ccttcgcggc acgccttgcc gcaacgctgg cggcgcagcg
     9541 accggccgag gtcgccgcgc cgcgggcgcc ggccctgtcg ccgcatgcgc tcacgcgacc
     9601 ggcggccaac gacggcctgt ttggctggcg catggtggcc ggactggcct cgtttgccgc
     9661 cgtggccgtg atcggctggg gcatgctggg cgatctcgga ccccagcgtc agggcggggc
     9721 gcagctggcg cagggcgctg ccgcgccgcg gaccgcgctg cagaccgtgg ccggtccgtc
     9781 gcctgcgctg agcaccgccg agccggatga aatccgggtg gccgacgggc cggcggtgat
     9841 gttgcgcgat ccgcgcctcg atgaactgct gcaggcgcat cgccaggtgt cggcaacgcc
     9901 ggcgctgggc ggtttcgtgc tcaacgccac ctacgagggt gcagggcgtt gaaggcgatc
     9961 gggtggggtc gggtccgcgg cggtttgcgc cgcgctgcgc tgctgtggtg tgtcggcggc
    10021 atggtcagtc tggcgggcgc gcagagcatc ggccagttcg gcggcggtgc gcccgccggg
    10081 gtggttggtc cgtcgcagcg cagcgtggcc gagtggctgg ccaggctgca acagggggct
    10141 cgggtgcctt cctacatcgg caccttcgtc gtgtcgtcgg cgtctggcgc catgtccagc
    10201 tcacgcatct ggcatgtgtg cgagggcgat gtgcaactgg agcgcgtcga tgccctgacc
    10261 ggcgtgccgc gttccaccct gcggcgcaac gacacggtga tgaccttcct gcccgatgcg
    10321 cgggtggtca cgttggatca gcgccagctg ggtgccgtgt tccccaacct gctcaatctg
    10381 gcgcaggggc tgccggtcgc cgactactat gacgcgcgtg aactggggcg cggccgcgtg
    10441 gccggctgcg atgccgagat cgtgcagctg ctgccgcgcg acgaatggcg ctacggctac
    10501 cgcatctgga gcgaacggcg caccggcctg gtgctcaaga cccagaccct gaacgcccac
    10561 ggccgggtgc tggagcaggc cgccttttcc gaggtgcagc tggacgcgcc ggtgcaggcc
    10621 gagcagctgc gccagatgat ggccaacacc cagggctacc gcatcgtcaa ggccgagcac
    10681 gccaggacca cgccgcaggc cgaaggctgg cagctgacga ccatggtgcc cggtttcgag
    10741 gcgcgcaact gctaccgccg tccgccttcg gccagcgcat cgctggtgca gtgcctgttt
    10801 tcggatgggc tggccacggt gtcgctgttc atgcaaccgc tcgatccggc gcggcaccag
    10861 caggaaggcg cggtcgccat gggcgctact cagaccctga cacgccgcgt ggtcgaccgc
    10921 tccggtcagt ggtgggtcac cgccgtcggc gaagtcccgc cagccaccct gcaggccttc
    10981 gtccgcgggc tggaacgcat tccctgagcg cggcgttcac ccccgccgcc cacgcaacga
    11041 tcaccgacat gcctttgctg aacatgaaca agctgtccct ggccgcgctg gccgtcccga
    11101 tgctgctgag cgccaccctg aatctgccgc tggccgcgca ggcccaggcg cccgcggcac
    11161 ccatcgtgcg tgggctgccg gacttcaccg atctggtcga gcaggtgggc ccggcggtgg
    11221 tcaacatccg caccgtcgag cgcgtgcgca ccggcatcgg gcgcaatggc ccgatgatgg
    11281 atgaggagat gcaggagttc ttccgacgct tcttcggcct gccgctgccc ggcgtgccgc
    11341 gccagggccc gcgccagcag gagccgcagg agccggccga gcgcgaggtg ccgcgcggcg
    11401 tggggtccgg cttcatcctc accgccgacg gcttcgtgat gaccaacgcc catgtggtgg
    11461 acggcgccga cgaagtgctg gtgacgctga ccgacaagcg cgaattcaag gctcgcatcg
    11521 tcggtgccga caagcgcacc gacgtggccg tggtcaagat cgaggccagc ggcctgccgg
    11581 ccgtgcgcat cggtgacgtc agccgcctga aggtgggcga atgggtgatg gccatcggct
    11641 cgcccttcgg tctcgacaac accgtgacag ccggcatcgt cagtgccaag cagcgcgaca
    11701 cgggcgacta cctgcccttc atccagaccg acgtggcggt caacccgggc aactcgggcg
    11761 ggccactgat caacatgcgc ggtgaggtgg tgggcatcaa cagccagatc tactcgcgct
    11821 ccggcggctt catgggcatt tcgtttgcca tcccgatcga cgaggccatg cgcgtgagcg
    11881 agcaattgcg cgccagcggt cgcgtcacgc gcggccgcat cggggtgcag atcgatcagg
    11941 tgagcaagga ggtggccgag tccatcggcc tgggcaagcc gcagggcgcg ctggtgcgtg
    12001 gcgtcgaatc cggctcgccg gccgacaagg cgggcgtcga ggcgggcgac atcatcaccc
    12061 gcttcgatgg ccgggtgatc gataaagcgt ccgatctgcc gcgcctggtg ggcaacacca
    12121 aacccggcac gcgcagcgcc ctcacggtgt tccggcgcgg ccagacgcgc gagctgaaca
    12181 tcaccatcgc cgagctggaa cctgagcaga ccgcgcgccg acccagccag cgcggcagcg
    12241 ccccacccaa ggcctctgaa gccacccagg tgctcggcct gaccgtcacc gaactcaccg
    12301 aagcccagaa gaaggagctg aaggtgcgcc acggcgtgcg cgtggaagcg gccaccgacg
    12361 ccggtgcgcg cgccggcctg cgcgaaggcg acgtgatcct gtcggtcggc aacgccgagg
    12421 tccgcagtgt gcgggacttc gaggcggcgg tggccaaggc cgagcgcggc aaacccatca
    12481 gcatgctggt gcagcgcggc gagctggtgc agttcgtgct ggtcaggcct ggtcgctgag
    12541 cctgcctggg ccggggcata acccgaaact gaggtcgggt atggggctat tcggggtgct
    12601 gacggccacc ccgaggcggc aggccagtcg ggcaggccgc ctgtggcacc gaagttacat
    12661 acgcgtttgg atagaatacg ggtcggtcaa aggtcgtgat tcgcatatga tccggccgtc
    12721 acaccgccat gtgggacgcg gttggatgat ccaccggatt tccacaagcc gctgaaagtt
    12781 gttcagaaat tatcctgcaa atcatgttga caacctgttg ataaatattc gttgcagcgt
    12841 cacaacgtgg cggagatgag attccgtatc gcttggccgg gtggtttttg cctacaatga
    12901 agtcccaacc atcgcagttg ccatagattg ttggtttgag ggcgcgccgc atcactggcg
    12961 cgcccttttt tcatgtgcgc atctcggtaa ttcaaatact tgccttcttt cgttgatgga
    13021 ccacatcaga aatttctcaa tcattgcgca catcgatcac ggcaagtcca cgcttgccga
    13081 tcggctcatc tcgcgttgcg gcggcctgtc cgaccgtgag atgagcgagc aggtgctgga
    13141 ctcgatggac atcgagaagg agcgggggat aaccatcaag gcccagaccg ccgcgctgca
    13201 ctaccaggcg cgcgatggca gggtctacaa cctcaacctg atcgacaccc cgggccatgt
    13261 ggacttctcc tacgaggtgt cgcgttcgct gtcggcgtgc gaaggggcgc tgctggtggt
    13321 cgatgccagc cagggcgtgg aggcgcagac ggtggccaac tgctacaccg cactggagct
    13381 cggcgtggaa gtgctgccgg tgctcaacaa gatggacctg ccgcaggccg acccggacaa
    13441 cgccaaggcc gagatcgagg acgtgatcgg catcgatgcc agcgaggcca tcgccatctc
    13501 ggccaagacc ggcatgggca tcgacgacgt gctggaacag atcgtcgcca aggtgccgcc
    13561 accgcgcggc caggcccagg cgccgctgcg cgccatgatc atcgattcct ggtttgacgc
    13621 ctacgtcggc gtggtgatgc tggtgcgcgt ggtcgacggc cgactggcca agggcgagcg
    13681 cttcaagatg atggccagcg gctcggccta tgaagccaac cagctcggcg tgttcacccc
    13741 ggcgcagcag gcgcgtgagg tgctggaagc gggcgaggtc ggctacatca tcgccggcat
    13801 caaggagctc aaggccgcca aggtgggcga caccatcacg ctggagaaga agctgccggg
    13861 caacctgggc ccggccgaca ggccgctgcc gggcttcaag gaaatccagc cgcaggtgtt
    13921 tgccggcctg tacccgaccg aggccaacca gtacgaccag ctgcgcgacg cgctggaaaa
    13981 gctccagctc aacgacgcct cgctgcactt cgagccggag gtcagccagg cgctgggctt
    14041 cggctttcgc tgtggctttc tgggtctgct gcacatggag atcgtgcagg agcgcctgga
    14101 gcgcgagttc gatcaggatc tgatcaccac cgcgcccagc gtggtgtacg aggtggaaaa
    14161 ggccgacggc gaggtgatcc aggtcgagaa cccctcgaag atgcccgacc agggcaagat
    14221 ccacgagatc cgcgagccca tcgtcacggt gcacctgtac atgccgcagg actatgtggg
    14281 tccggtgatg acgctggcca accagaagcg cggcgtgcag atcaacatgc agtaccacgg
    14341 ccgccaggtc atgctgacct acgagctgcc gctgggcgag atcgtgctcg acttcttcga
    14401 taagctcaag agcgtcagcc gcggctatgc ctcgatggac tacgagttca aggaatatcg
    14461 cccctccgac gtggtcaagg tcgacatcct gctcaacggc gaaaaggtcg atgcgctgtc
    14521 gatcatcgtg caccgcagtc aggcgcagtt ccgcggccgg gcggtggcgg ccaagatgcg
    14581 cgagatcatc agccgccagc agttcgacgt ggcgatccag gcggccattg gtgccaacat
    14641 catcgcgcgc gagaacatca aggcactgcg caagaacgtg ctggcaaaat gctatggggg
    14701 tgacatcacc cgcaagcgca aactattgga aaaacagaag gcgggcaaga agagaatgaa
    14761 gcagatcggt tcggtcgagg tgccgcagga ggcattcctc gccatcttgc aggtggagga
    14821 ctgatgccct tgctgaccgc cgtcattctg gccgccttcg tggcctacgc cgccgcctgg
    14881 tacgtcggca tccttcaggg caacttcgcg ttgctgctgc tgctggccac gctggtgacc
    14941 ggtgcgtact ggctgggcga gcgcttcgtg tttctgccgc gccgtcgtgc cgccgcccag
    15001 gccctggagg cgcaggcggc ccagcgccgc agcgagctgg cggcccaggg catcacccag
    15061 gtggacgagg gcgagctgac ccaggcgcgc gagcgcgtgc tggcccagcc ctggtggctg
    15121 gactggacgg ccggcctgtt cccggtgatc gcggtggtgt ttctgctgcg ctcgttcgtg
    15181 ttcgagccgt tcaagattcc ctccggctcc atggtgccga ccctgctggt gggcgatctg
    15241 attctggtga acaagtacac ctatggcctg aagctgccgg tgatccacac ccgcctgact
    15301 cagggccagc cggtgcagcg cggcgacgtg gtggtgttcc gctatccacc gcagcccagc
    15361 gtggactaca tcaagcgcgt ggtcggcctg cccggcgacg aggtggccta cctcaacaag
    15421 cagctcacca tcaatggcca gccggtgccg cagacggcgg tgtccgactt cttcgacgaa
    15481 caggcgatgg aatacttcaa gcagttcgac gaacgcctgg gcgtgacaaa tcaccggatc
    15541 atcgtcgacg ggcggcgtcc ggctttcgtg ggcggggctt cggacttcga atacaaggac
    15601 aattgccgct atagcgtcga gggtgtccgc tgcaaggtgc cgcccgggca ttacttcatg
    15661 atgggcgaca accgcgacaa ttcgctcgat tcgcgcttct ggggcttcgt gcctgacggc
    15721 cacatcgtcg gcaaggcgtt cttcgtctgg atgaacttcg gcaacctcaa gcgcattggc
    15781 tccttccatt gatcctttcc gacccttcgc gagaaaaatg aaacatcaag gtccatcgtc
    15841 ccagcgtggc atttcctttt tcggcctgct gttcctcggc gtcgtgctgg cgctgctggc
    15901 catcgtcggt gcccgggtgg tgcccacggc gtccgagttc atgacgatcc agaaggcggt
    15961 caagaaagcc gccgccgacg gcgacaccgt gcccgccgtg cgcgcctcgt tcgaccgcac
    16021 cgcctcggtg gactacatca cctccatctc cggcaaggac ctggaggtca ccaagcaggg
    16081 cgacaaggtg gtggtcaact tcgcctacga caaggaaatc ccgctgtttg gtccggccta
    16141 cctgctgatc aagtaccgtg gctcctccgc gggtggctac gactgagcgt ccgccgacgt
    16201 ccgcgcccgg ctcgtcgggt cgcgcacgtg cgccctggaa ggccctgcag gagcgtctgc
    16261 agtaccactt tcgggatgag tcgctgctcg agcgggcgct gacgcaccgc agcttctcgg
    16321 ccgaccacaa cgagcgcctg gagttcctgg gtgactcggt gctgggtctg ggtgtggccc
    16381 acatgcttta ccaggcgctc ggcgggggtg ccgaaggcga tctgtcgcgc ctgcgcgccc
    16441 agctggtgcg gcaggacagc ctgcatcagc tggcgctcca cctgggtctg ccgccactgt
    16501 tgcgcctggg cgagggcgag ctgcgctcgg gcgggcagcg ccggccctcg atcctggccg
    16561 acgcgctgga ggccctgatc ggcgccgtct acctggacgg cggccacgcg cccgccgacg
    16621 cactggtgcg ccggctgttc gagcaggtgg agatttcccc cgccatgtcg gccgccgcca
    16681 aggacgccaa gaccgccttg caggaatggc tgcaggcgcg caagatgcgc ttgccccagt
    16741 acgaggtggt gcgcgtgctc ggcgccgcgc accgtcagac cttcgaggtg cggtgccagg
    16801 tggccgaaca gaacctggaa gtgctagggc agggcgcctc gcgccgtgcc gccgagcagt
    16861 gcgccgccgc gtcgatgctg gagcaactcc agaaccgtcc atgaatactc ctgaaatcat
    16921 agctgacggc gctgatccag agcgcgctgg agcccgatct ggcactccat ccgcgcccga
    16981 tcccggggcc agcgtgcagc gctgcggcct gatcgccatc gtcggcaagc ccaacgtcgg
    17041 caagtcgacc ctgctcaacg ccctggtggg gcagaagatc agcatcacca gccgcaaggc
    17101 gcaga
//
LOCUS       DAOOOG010000035        16717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_3620_length_16717_cov_23.113012, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000035 DAOOOG010000000
VERSION     DAOOOG010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 16717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16717
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3620_length_16717_cov_23.113012"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PK242_04550"
     CDS             <1..683
                     /locus_tag="PK242_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ93320.1"
                     /translation="DWRARTGHTILERYGMSETIMLTSNPWQADARYGGQDERRGGTV
                     GFPLPGVRLRVQGEDGRDLPAGEIGGIQVKGPNVFAGYWQMPEKTAEEFTSDGFFKTG
                     DVGQQDARGYVTIVGRSKDLIISGGYNVYPAEIEGFLNELPGVAESAVVGVPHPDFGE
                     VGVAVVIARPGARPDADALLAELKARLANFKIPKRCFVETELPRNTMGKVQKNLLRQR
                     YQHLFVTA"
     gene            794..1405
                     /locus_tag="PK242_04555"
     CDS             794..1405
                     /locus_tag="PK242_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOZ93321.1"
                     /translation="MSLRALLLLLVGTAIVAFIGINWSAMTAPTDLNLVFMEIRAPLG
                     LVLLGLMALLSAVFVGLIAYTQGTVLMETRRHAKELSVQRELADKAEASRFTELRAHL
                     DREVSRLSEVIANQTRETLSRVDRAETGLRDRPVDAEIGRLVQTVEGHNRELHARIDR
                     LEIGLRERLAGQLPAPTPRPTALPNAAPLQAGGGESAGAASAS"
     gene            complement(1497..1940)
                     /locus_tag="PK242_04560"
     CDS             complement(1497..1940)
                     /locus_tag="PK242_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ93322.1"
                     /translation="MYDRILVATDGSKLSKKAVDSAIKLAALCGAELIALRVVPRYPQ
                     SYFEGSIPLAPDDVARVEKQWADQGQESVDAVKKAGEAKGVKVKAILAKSDIVSDALI
                     AAARKHKADIIVMASHGRKGVKRLLLGSETQQVLTHSEIPVLVLR"
     gene            2187..4424
                     /locus_tag="PK242_04565"
     CDS             2187..4424
                     /locus_tag="PK242_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOZ93323.1"
                     /translation="MLDERDEWLEFSRPAGAQGGLWESSVVFEGMHCAACAVTIEQAL
                     RATPGVREADISAASHRGRVVWEEGATRPSAWMQAVLRSGYRPLPAHDAFASERRQAE
                     TRRMLWRLGVAGVCMMQVMMYATPTYLASPGEIEPNFIHLLRWAQWVLSIPVVLFSCQ
                     PFFRHALLDLKLRRVSMDLPVALGMLITFVVSSLGTFEPEGLFGREVYFDSLTMFVFF
                     LLTGRWLELRLRDRTAGALEALMHRLPDSVLRQAADGAWERVSVRRVRVDDVLRVLPG
                     EVFPADGVLLRGQTAVDEALLTGESTPLSRGEGAPVIAGSHNLSAAVEMRVEQVGADT
                     RYAQIVALMAQASTSKPQIAQLADRLAKPFLIFVLLAAGLACAWWWPGDPAHALMIAV
                     AILVVTCPCALSLATPAAMLASAGALARRGVLVRRLSALEALSKVDTVVFDKTGTLTR
                     DAFVLEQVRLRDGVARAQALALAAAMAQGSLHPLSRALVQAAQDEGVEPLAVADLQEM
                     VGQGLTARGPATGPLRGQVRLGAAAWCGVQGSDAGGPVSHLSDEQGWVASFEMREHLR
                     PDARAAVEALRAAGVRVRLLSGDRPDAVRRVGQLAGIDDVQGSCLPDDKLAQMRQAQG
                     AGAVVAMVGDGLNDGPVLAGADVSFAFGHAVPLARAQADFVVLGERLGLISGSFAVAR
                     RTMRIVRQNLGWAAAYNLVGVPLAMAGWMSPWAAGLGMAASSLLVVVNALRLTKAPPM
                     PHPEH"
     gene            4424..4561
                     /gene="ccoS"
                     /locus_tag="PK242_04570"
     CDS             4424..4561
                     /gene="ccoS"
                     /locus_tag="PK242_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HOZ93324.1"
                     /translation="MDILYLLIPLSVVLVFFILAALWWAIYRGQFDDVEREGERIFEH
                     D"
     gene            4629..6065
                     /gene="ccoN"
                     /locus_tag="PK242_04575"
     CDS             4629..6065
                     /gene="ccoN"
                     /locus_tag="PK242_04575"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HOZ93325.1"
                     /translation="MTFANTQATVYNDKVVRQFAIMTIVWGVVGMLVGVIIAAQLAWP
                     ELNFGIPWLSYGRLRPLHTNAVIFAFGGSGLIGTSYYVVQRTCQVRLFSDKLAAFTFW
                     GWQLVIVAAAISLPLGYTTSKEYAELEWPIDVLITLVWVAYAVVFFGTVGTRRVRHIY
                     VANWFYGGFILAIALLHVVNSAELPVGLMKSYSAYAGVQDAMVQWWYGHNAVGFFLTA
                     GFLGMMYYFIPKQAERPVYSYRLSIVHFWALIFTYMWAGPHHLHYTALPDWAQSVGMI
                     FSLILLAPSWGGMINGIMTLSGAWHKLRDDPILRFLIVSLSFYGMSTFEGPMMSIKTV
                     NALSHYTDWTVGHVHSGALGWVGLITMGSMYYLIPRLFGQKQMHSVKAIEVHFWVATV
                     GIVLYIAAMWIAGVMQGLMWRAVNPDGTLTYTFVESVKATYPFYVLRLMGGLLYLGGM
                     LIMLWNTIKTATAGRVVPVQIPAVAAHA"
     gene            6083..6700
                     /gene="ccoO"
                     /locus_tag="PK242_04580"
     CDS             6083..6700
                     /gene="ccoO"
                     /locus_tag="PK242_04580"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HOZ93326.1"
                     /translation="MAQEKVTGHAKVETSNFLMIVLILLVVSIGGLVEIVPLFFQKST
                     TQPIEGLKPYTALQVVGRDVYVREGCYNCHSQMIRPFRAEALRYGPYSVAGEFVYDHP
                     FQWGSKRTGPDLARVGGRYSDDWHRAHLNNPRDVVPESNMPAYPWLERTAANAGTIQA
                     HMRGLRTLGAPYTDEEIAKAPEEVKGKTEMDAVIAYLQVLGIHRK"
     gene            6721..6861
                     /locus_tag="PK242_04585"
     CDS             6721..6861
                     /locus_tag="PK242_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HOZ93327.1"
                     /translation="MDINLLRSIVTVLSFVLFIGIVVWAWSARNRERFDEAAQLPFEQ
                     QD"
     gene            6895..7782
                     /gene="ccoP"
                     /locus_tag="PK242_04590"
                     /pseudo
     CDS             6895..7782
                     /gene="ccoP"
                     /locus_tag="PK242_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016722100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            7876..8172
                     /locus_tag="PK242_04595"
     CDS             7876..8172
                     /locus_tag="PK242_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ93328.1"
                     /translation="MVKFFFATGKADLAPEADKALADILEGVKAGKKAVISGFVDSTG
                     NAEQNAELAKQRAIAVRDQLKALGVGEDQVELKKPANIDAGADAQARRVEVSLG"
     gene            8461..9828
                     /gene="ccoG"
                     /locus_tag="PK242_04600"
     CDS             8461..9828
                     /gene="ccoG"
                     /locus_tag="PK242_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOZ93329.1"
                     /translation="MVSLYQAHKKVYPRSVTGLFAKWRWFFVFATQIFFYGMPWLQWG
                     ERQAMLFDLEARRFYIFGLVLYPQDVIYLTAILIISALSLFLFTAVAGRQWCGYACPQ
                     TVYTEIYLWFERMIEGDRSARMRLDAAPMSAAKFGRKALKQAVWIAFGLWTGFTFVGY
                     FTPIRELAQHVLQWNLGAWEIFWIFFYGFATYGNAGFMREQVCMYMCPYARFQSAMFD
                     KDTLIVTYDAKRGEPRGGRSKSADPKALGLGDCVNCTLCVQVCPTGIDIRNGLQYECI
                     SCAACIDVCDEVMDKVGYPRGLIRYSTEHALEQGWGRKEILRQVLRPRVLIYVAILSA
                     VVVALIASLVLRTPFKVDIVRDRATLARLVDGNQIENVYRIQVMNATEKPQTFQLSVE
                     DLPGLEIASDKSVTVDGAQSLWVPVQLRLPYGSASSGSHTIHFRVTAPGVGEVREKSI
                     FMVPR"
     gene            9834..10127
                     /locus_tag="PK242_04605"
     CDS             9834..10127
                     /locus_tag="PK242_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein FixH"
                     /protein_id="HOZ93330.1"
                     /translation="MSDPASTPPSPWWKFGHMWLVIAGPLIVVLASIVTIYLAVRTPD
                     PVYGDASRGAARSSQAKDGGDAGRMSPAMQARNHAATGGVARPAPNHGTGPNQ"
     gene            10165..10422
                     /locus_tag="PK242_04610"
     CDS             10165..10422
                     /locus_tag="PK242_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93331.1"
                     /translation="MLRERLMWIAWPAFLMAAVLEFMVFAVLDPQALTLLGEPSGWSR
                     EGVYTVTFFIFWAVMMLSGALTTLLSKSPFDVNRRQSRALR"
     gene            complement(10509..10605)
                     /gene="ffs"
                     /locus_tag="PK242_04615"
     ncRNA           complement(10509..10605)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK242_04615"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(10611..10853)
                     /locus_tag="PK242_04620"
     CDS             complement(10611..10853)
                     /locus_tag="PK242_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93332.1"
                     /translation="MMDVFESHDTIVIQTDADPRIAALVGALTDCIHPRAARLKARLA
                     ADPDDEQLGQLRAEVLNLVAMSFGLAEAERRLQAVQ"
     gene            11024..12979
                     /gene="dnaX"
                     /locus_tag="PK242_04625"
     CDS             11024..12979
                     /gene="dnaX"
                     /locus_tag="PK242_04625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOZ93333.1"
                     /translation="MSYLVLARKYRPRNFSEMVGQEHVVQALTNALTQQRLHHAYLFT
                     GTRGVGKTTVSRILAKSLNCTGADGQGGITATPCGVCPACADIDAGRFVDYTELDAAS
                     NRGVDEVQQLLEQAVYKPVQGRFKVFMIDEVHMLTNTAFNAMLKTLEEPPEYLKFVLA
                     TTDPQKVPVTVLSRCLQFNLRPMAPETVLDHLTRVLAAEAVAAEPAALRLLARAARGS
                     MRDALSLTDQAIAFGSGELRESAVRQMLGSVDRSVVFSLIGALADGDGRTVVTLSEQL
                     RASGHSAAQTLEDMASVLQRMAVQQAVPDLAVDASDPDAQETARLAARLPADETQLLY
                     SLCIHGRSELGLAPDEYTGLTMALLRLLPFRQPVPTQAQTVSATAEKKSLKTPEASPA
                     PQGASAPPAKAPAPAPVAASDDPAAAEQGAAEPGAAADARPQPVTERDAELDTQGAPP
                     ARPVPAPRPQPALQPLPVRPSNGTRFQEQNQPPAPDHIAPVAPETIAIQVREGDQEPA
                     PAPRLRVDAPPPPVVPGAEGDFWHALVQELVAREAVTALVRELALQSQLVARTDRQWT
                     LRVESESLAQSGVRERLQAALADAGHCVRLQIDSGPVSDSPARRNAIHATRRLKAAEA
                     LLLADPFVQEMMRDFGAKIVPGSIKPL"
     gene            13100..13435
                     /locus_tag="PK242_04630"
     CDS             13100..13435
                     /locus_tag="PK242_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOZ93334.1"
                     /translation="MFNKGQLAGLMKQAQAMQDNLKKAQDELALMEVEGESGAGLVKV
                     LMTAKHDVKRVTIDPSLLADDKDMLEDLVAAAFNAAVRKAEELSAEKMGKLMPAGMPG
                     LPGGMKFPF"
     gene            13580..14170
                     /gene="recR"
                     /locus_tag="PK242_04635"
     CDS             13580..14170
                     /gene="recR"
                     /locus_tag="PK242_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOZ93335.1"
                     /translation="MSDTHSLDTLIQALRRLPGVGQRSAQRMAFHLLQHDREGAQQLA
                     RALHEAVGQVRHCARCHTFTEAEVCATCSDPRRDASQLAVVETPADQAALERTGAFRG
                     RYFVLMGRLSPLDGIGPRDIGVQRLIERACDGVVREVILATNFTAEGEATAHLIGQAL
                     RSRGLAVTRLARGVPAGSELEYVDLGTIAHALVDRR"
     gene            14177..14932
                     /locus_tag="PK242_04640"
     CDS             14177..14932
                     /locus_tag="PK242_04640"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOZ93336.1"
                     /translation="MSLSASILAARQLLQEAAHIAVLSGAGISAESGVPTFRDAQTGL
                     WAHLRPEDLATEDAFRRHPQRVWDWYAERRASLAEVQPNAGHRALAEFQHRHPGRLTV
                     ITQNVDGLHQRAGSTGVLALHGSLAADRWLDPCPAGRGPTACDVARADPGRPPRCAQC
                     GNWVRPAVVWFGELLPEDQLRQAEQAASRCDAMLVVGTAGAVYPAAGLAHQARAAGAR
                     LIVVNPHPSELDGLAQLRLVGGAAQVLPPLLDF"
     gene            complement(15076..15765)
                     /locus_tag="PK242_04645"
     CDS             complement(15076..15765)
                     /locus_tag="PK242_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HOZ93337.1"
                     /translation="MLIHLKNLLSTDEVAHARRLLLADDAPWVDGARSAGGQAVQRKS
                     NRQLAQDSPSAATLRELVLAALRRDPVFFSAALPRRIFNPLFNRYEGVANHYGQHVDG
                     AVLHSQTSGEWVRSDLSCTVFLTEPDDYDGGELVVHDTFGPQAVKLPAGDAVLYPGTS
                     VHEVRPVTRGARIASFLWIESMVRGQEQRRLLFEMDMALLALRQRHGETDEAIRLTGT
                     YHNLLRMWAST"
     gene            complement(15834..>16717)
                     /locus_tag="PK242_04650"
     CDS             complement(15834..>16717)
                     /locus_tag="PK242_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ93338.1"
                     /translation="LQDTVSLTDTVKLVGGLRFDHFKGSYRNADGSLSNARTDNLWSP
                     RLGLLYQPDEASSYYISYGTSFNTSGDAYQYGVLINPNTGRSAKTPPEKSRNIEIGGK
                     WDLFDKRMLAGAALFYSEKYNERNTDPDTAATQELLSGKRHAAGMEFNLAGRITPAWE
                     LFWNHTWIPKAKIDKSNVVLNPAGTGAQVQGDRPGLTPRHSGSVWTTYRVMPQLRLGL
                     GANYRSSQNPEGQRTLKAKGFVTFDAMAEYSFTEATTLKLSVTNLSNKLYADGLYRGF
                     YQPGAPRRVELTLKHLF"
BASE COUNT         2571 a         5482 c         5866 g         2798 t
ORIGIN      
        1 acgactggcg cgcgcgcacc ggccacacca tcctggagcg ctacggcatg agcgaaacca
       61 tcatgctgac cagcaacccc tggcaggccg acgcccgcta cggcggccag gacgagcggc
      121 gcggcggcac ggtcggcttt ccgctgccgg gcgtgcgcct gcgcgtgcag ggcgaagacg
      181 gtcgcgatct gccggcgggc gaaatcggcg gcatccaggt caagggcccg aacgtgttcg
      241 ccggctactg gcagatgccc gaaaagaccg ctgaggagtt cacgtccgat ggcttcttca
      301 agaccggcga cgtcggccag caggacgcgc gcggctacgt caccatcgtc gggcgcagca
      361 aggacctgat catcagcggt ggctacaacg tctacccggc cgagatcgaa ggcttcctga
      421 acgagctgcc cggcgtggcc gaaagcgccg tggtcggcgt gccgcacccg gacttcggcg
      481 aagtcggcgt ggccgtggtg atcgcccggc ccggcgcccg gcccgacgcc gatgccctgc
      541 tggccgagct gaaggcgcgg ctggccaact tcaagatccc caagcgctgc ttcgtcgaaa
      601 ccgaactgcc gcgcaacacc atgggcaagg tgcagaagaa cctgctgcgc cagcgctacc
      661 agcatctgtt cgtcacggcg tgaagcttgc gaccgtcagg cgtggctgtc aggcgtggca
      721 ggggcacggg taaacttgcc cgctgatggt cacgcgagcc gcgtgcagat tcccaggaag
      781 gagccccacc gccatgtccc tgcgagccct gcttctgctg ctggtcggca ccgccatcgt
      841 ggccttcatc ggcatcaact ggagcgccat gacggcgccc accgacctga acctggtgtt
      901 catggaaatc cgcgccccgc tcggtctggt gctgctgggg ctgatggcgc tgctgtcggc
      961 cgtgttcgtc ggcctgatcg cctacaccca gggcacggtg ctgatggaaa cgcgccgtca
     1021 cgccaaggag ctgtcggtgc agcgtgaact ggccgacaag gccgaggcct cgcgcttcac
     1081 cgaactgcgc gcccacctgg accgcgaggt gagccggctg tccgaggtca tcgccaacca
     1141 gacacgcgag acgctgtcgc gcgtcgaccg ggccgaaacg gggctgcgcg atcgtccggt
     1201 ggacgccgag atcggccgcc tggtgcagac cgtggaaggc cacaaccgcg agctgcacgc
     1261 ccgcatcgac cggctcgaga tcgggctgcg cgagcgcctg gccgggcagc tgcccgcccc
     1321 gaccccaagg cccaccgcac tgcccaatgc ggcgccgctg caagccggtg gtggcgaatc
     1381 ggcgggcgcg gcgagcgcgt catgacgcag cgggctggcc gcatgtgacg acggccccgg
     1441 gtggcgcccg gagccgtcgt tcgggggggt gcgcggccgc ggcggccggc gcacggtcag
     1501 cgcagcacca gcactgggat ttccgagtgc gtcaacacct gctgcgtctc gctgcccagc
     1561 agcaggcgct tgacgccttt gcggccgtgc gaggccatga cgatgatgtc ggccttgtgc
     1621 ttcctggctg cagcgatcag ggcatcggag acgatgtcgg acttggccag aatcgccttg
     1681 accttgacgc ccttggcctc gccggccttc ttgaccgcgt ccacggactc ctggccctgg
     1741 tcggcccatt gcttttcgac gcgcgccacg tcgtccggcg ccagcgggat ggagccctcg
     1801 aagtagctct gcgggtagcg cggcacgacg cgcagcgcaa tgagttcggc gccacacagg
     1861 gcggccagct tgatggcgct gtccaccgct ttcttggaca gtttggagcc gtcggtggcg
     1921 acaaggatgc ggtcgtacat ggtggggatc tccagtgttg aggtatcggc gggcggcgcc
     1981 ggggagcttg cgcggatcag cggatagctt agcgcactcc gatcgcggtg ggcggtcatt
     2041 ggcgcgccct ggccggcgca ggcgccaaaa cgcgtacagt tcgtgccgtg atgcaaggcg
     2101 caagtgggac tttcccgggc tcggtggcgg gcgatgggcc cgggcagcgg ccatcggggc
     2161 cgccgcaggg cgatgccgcg tggcagttgc tcgacgaacg cgacgagtgg ctggagttca
     2221 gccgcccggc aggcgcgcag gggggactgt gggaatccag tgtggtgttc gaaggcatgc
     2281 attgcgcggc ctgcgcggtg accatcgaac aggccctgcg cgccacgccc ggcgtgcgcg
     2341 aagccgacat cagcgccgcc agccatcgcg gtcgggtggt gtgggaggag ggcgccaccc
     2401 gtccttccgc ctggatgcag gcggtgctgc gcagtggcta ccggccgctg ccggcccacg
     2461 atgccttcgc cagcgagcgc cgtcaggccg aaacccgccg catgctgtgg cgcctggggg
     2521 tggccggtgt ctgcatgatg caggtgatga tgtatgccac gccgacctac ctggcgtcgc
     2581 ccggcgagat cgaaccgaac ttcatccacc tgctgcgctg ggcgcagtgg gtgctgtcga
     2641 ttccggtggt gctgttctcg tgtcagccct tctttcgaca tgcgctgctc gacctgaagc
     2701 tgcgccgcgt cagcatggac ctgccggtgg cgctgggcat gctgatcacc ttcgtggtca
     2761 gttcgctggg cacgttcgag cccgaggggc tgttcgggcg cgaggtctat ttcgactcgc
     2821 tgaccatgtt cgtgttcttc ctgctcaccg gccgctggct ggagctgcgc ctgcgcgatc
     2881 gcacggcggg cgcactggaa gccctgatgc accgcctgcc cgacagtgtg ctgcgacagg
     2941 cggcggacgg cgcatgggag cgcgtgtcgg tgcggcgcgt gcgcgtcgac gacgtgctgc
     3001 gggtgctgcc gggcgaggtg tttccggccg atggggtgct gctgcggggc cagaccgcgg
     3061 ttgacgaagc cttgctgacc ggcgaatcga ccccgctgtc gcggggcgag ggtgcgcccg
     3121 tcattgccgg cagccacaac ctgtcggcag cggtggagat gcgggtcgag caggtcggcg
     3181 ccgacacccg ctacgcgcag atcgtggccc tgatggccca ggcgtcgacc agcaagccgc
     3241 aaattgccca gctggccgac cgcctggcca agccatttct catcttcgtg ctgctggcgg
     3301 ccggcctggc ctgcgcctgg tggtggcccg gcgatccggc gcatgcgttg atgatcgcgg
     3361 tggcgatcct ggtggtgacc tgcccgtgcg cgctgtcgct ggcgacgccc gccgccatgc
     3421 tggccagcgc cggcgcgctg gcgcgccgtg gcgtgctggt gcgtcgcctg tcggcactgg
     3481 aagcgctgtc gaaggtggac acggtggtgt tcgacaagac cggcacgctg acgcgcgatg
     3541 ccttcgtgct ggagcaggtg cgcctgcgcg acggcgtggc gcgcgcgcag gcgctggcgt
     3601 tggcggcggc catggcgcag ggcagcctgc atccgctgtc gcgcgcgctg gtgcaggcgg
     3661 cgcaggacga gggggtcgag cccctggcgg tggccgatct gcaggaaatg gtggggcagg
     3721 gcctgacggc ccgtggccct gcaaccgggc cgctgcgcgg tcaggttcgc ctgggggcgg
     3781 cggcatggtg cggcgtgcag ggcagcgacg cgggcggtcc ggtcagtcat ctgagcgacg
     3841 agcagggctg ggtggccagc tttgagatgc gcgagcatct gcgcccggac gcgcgcgctg
     3901 cggtcgaggc gttgcgcgcg gccggcgtca gggtgcggct gctgtcgggc gaccggccgg
     3961 acgcggtgcg gcgcgtcggc cagctggccg gcatcgacga tgtgcagggc agctgtctgc
     4021 ccgacgacaa gctggcgcag atgcgtcagg cccagggcgc cggggcggtc gtggccatgg
     4081 tcggcgacgg cttgaacgac gggccggtgc tggcgggcgc cgacgtgtcg ttcgcgttcg
     4141 ggcacgccgt gccgctggcg cgggcgcagg ccgacttcgt ggtgctgggc gagcgcctgg
     4201 ggctgatttc ggggtctttt gccgtggcgc gccgcaccat gcgcatcgtg aggcagaatt
     4261 tgggctgggc cgcggcctac aacctggtgg gcgtgccgct cgccatggca gggtggatgt
     4321 cgccctgggc ggcaggcttg ggcatggcgg ccagttcgtt gctggtggtg gtcaatgccc
     4381 tgaggctgac caaggccccg ccgatgccgc atccggagca ctgatggaca ttctttatct
     4441 gctgattccg ctgtcggtgg tgctggtgtt tttcatcctc gccgcgttgt ggtgggccat
     4501 ctaccgtggc cagttcgacg acgtcgagcg cgagggcgag cggattttcg agcatgattg
     4561 acgcacgtca atcaaaacca caatagcttc gcggacactg cgtagggttt ttcaagtaag
     4621 aggtggaaat gacattcgcc aacacacagg ctactgtcta taacgacaaa gtggtcaggc
     4681 aattcgccat catgacgatc gtctggggcg tggtggggat gctggtcggc gtgatcatcg
     4741 ccgcgcaact ggcatggcct gaactgaact tcggcattcc atggctcagc tatggccgct
     4801 tgcgaccgct gcacaccaac gcggtgatct tcgccttcgg cggcagcggc ctcatcggaa
     4861 cctcgtacta cgtggtgcag cgcacctgcc aggtgcggct gttctccgac aagctggcgg
     4921 cattcacgtt ctggggctgg cagctggtca tcgtcgccgc ggccatttcc ctgccgctcg
     4981 gctacaccac cagcaaggag tacgccgagc tggagtggcc gatcgacgtg ctcatcaccc
     5041 tggtctgggt ggcctacgcg gtggtgttct tcggcaccgt cggcacgcgc cgcgtgcgcc
     5101 acatctatgt ggccaactgg ttctacggcg gcttcattct ggccattgcg ctgctgcatg
     5161 tggtcaacag cgccgagctg ccggtcggac tgatgaagtc ctactcggcc tacgccgggg
     5221 tgcaggatgc gatggtgcag tggtggtatg gccacaacgc cgtgggcttc ttcctgaccg
     5281 ctggtttcct ggggatgatg tactacttca ttcccaagca ggccgagcgc ccggtctaca
     5341 gctatcggct gtccatcgtg cacttctggg cgctgatctt cacctacatg tgggccggtc
     5401 cgcaccacct gcactacacc gcgctgcccg actgggcgca gtcggtgggc atgatcttct
     5461 cgctgatcct gctggcgccc agctggggcg gcatgatcaa cggcatcatg accctctcgg
     5521 gcgcctggca caaactgcgt gatgacccga tcctgcgctt cctgatcgtc tcgctgtcgt
     5581 tctacggcat gtcgaccttc gaggggccga tgatgtcgat caagaccgtc aacgccctct
     5641 cgcactacac cgactggacc gtgggccacg tccactctgg tgccctgggc tgggtcggcc
     5701 tgatcaccat gggctcgatg tactacctga tcccgcgtct gttcgggcag aagcagatgc
     5761 acagcgtcaa ggccatcgaa gtccacttct gggtcgccac cgtgggcatc gtgctctaca
     5821 tcgccgcgat gtggattgcc ggtgtgatgc agggcctgat gtggcgcgcc gtcaaccccg
     5881 acggcaccct gacctacacc ttcgtcgaat cggtgaaggc gacctacccc ttctacgtgc
     5941 tgcgtctgat gggtggcctg ctgtatctgg gtggcatgct catcatgctg tggaacacca
     6001 tcaagacggc cacggcgggc cgtgtggtgc cggtgcagat ccctgcggtt gccgcgcacg
     6061 cctgaggagg agttgagcag tcatggcaca agaaaaagtc accggccacg ccaaggtcga
     6121 aaccagcaac tttctgatga tcgtgctgat tcttctggtg gtgtccatcg gcggattggt
     6181 cgaaatcgtt ccgctgttct tccagaagtc caccacccag ccgatcgagg ggctcaagcc
     6241 ctacaccgcc ctgcaggtgg tcggccgcga cgtctatgtg cgcgagggtt gctacaactg
     6301 ccactcgcag atgatccggc cgttccgcgc cgaggcactg cgctacgggc cgtactcggt
     6361 ggccggcgag ttcgtctacg accacccgtt ccagtggggc agcaagcgca ccggccccga
     6421 cctggcccgc gtgggtggac gctacagcga cgactggcac cgcgcccacc tgaacaaccc
     6481 gcgtgacgtg gtgcccgaat cgaacatgcc tgcctatccg tggctggagc gcaccgccgc
     6541 caacgccggc acgatccagg cccacatgcg tggtctgcgc accctgggcg caccgtacac
     6601 ggatgaggaa atcgccaagg cgccggaaga ggtcaagggc aagacggaga tggatgccgt
     6661 gattgcctac ctgcaggtgc tgggcatcca tcgcaaataa gcagcatggg agcctccgtc
     6721 atggacatca acctgttgcg cagcattgtc actgtgctgt ccttcgtgct cttcatcggc
     6781 atcgtggtct gggcctggtc cgcacgcaac cgagagcgct tcgacgaagc ggcgcaactg
     6841 ccattcgagc agcaggattg acccggagca acccgacagg caaggaattc aaaaatgagc
     6901 gactttacga gcaatttctg gtcggtgtac gtggcgggaa tcacgatcgc cggcatcatc
     6961 ggctgcctgc tgctgctgtg ggtcagtggc aagaccaagg ccatgaccga cagcgacaac
     7021 accaccgggc atgtgtggga tcaggatctg cgggagatga acaacccgct gccgcgctgg
     7081 tgggtgggcc tgttcatcat cacctgcgtg ttttcgctgg cgtacctgtt cctgtacccg
     7141 ggtctgggca cctaccaggg ttccctgaac tggtcgcaga ccgatcagtt cgagcgtgag
     7201 gtggcgcgtg gcaacgagca ggtggcgccc atctatgccg cgtttgccgg caagagcgca
     7261 gccgagctgg ccaaggatcc gcaggccatg gcgatcggcg atcgcctgtt catgaacaac
     7321 tgcgcccagt gccacggctc ggacgcgcgc ggctccaccg gttttcccaa cctgaccgac
     7381 ggtgactggc tgtggggcgg caagccggag cagattcacg aatccatcac caaggggcgc
     7441 accggcgtga tgaccccgat gggcgcggcc gtgggcagtg gggacgacgt gcgcaatctg
     7501 gccaactacg tgctcagcct gtcgggcggt ccgcacgatt cggtgcgcgc caacctgggc
     7561 aagcccaagt ttgcggtctg tgcggcctgc cacggggccg atggcaaggg caacccgctg
     7621 ttgggtgctc ccaacctgac cgacaacatc tgggtgcatg gtccgggcgt ggagtcgcat
     7681 gtcgtcaaca tgatcaacaa cggcaagacc ggcgtcatgc ctgcgtggga aagcaagttc
     7741 acacccgagc aactggcggt gttgacggcc tatgtctggg gcaagggcgg cggtgtggcg
     7801 gatgccgccg cggccgccac ctcgaaggcg ggcgcagccg ccgccgatac ggcctccgtg
     7861 gtggtcgaag gtggcgtggt caagttcttc tttgccaccg gcaaggccga tctggcgccc
     7921 gaggccgaca aggcgctggc cgacatcctg gaaggggtga aggcaggcaa gaaggccgtg
     7981 atcagcggct tcgtcgattc caccggcaat gccgagcaga atgccgaact ggccaagcag
     8041 cgggcgatcg cggtgcgtga ccagctcaag gcgctggggg tgggcgaaga ccaggtggag
     8101 ctgaagaagc cggccaacat cgatgccggc gccgacgccc aggcgcgtcg cgtcgaagtc
     8161 tccctcggct gaggtgaaac cgtgcggcgg gcgatggaaa tcttcctcgc ggccgcctcc
     8221 gggggcgggt agacccggct tggtgaccac atcacaatca caacccgcag gagacagagt
     8281 ggattcgggg ggcgcatggc gccccgtttt ccttggtcag gtggttgacg tcggcgccgc
     8341 ctggatcatc gcccgttcag ggtccgaatc ctgcatgggc cgaccactga cggacaggca
     8401 ggcgtgagca atccaacccc atctccgaag aaggtcgcgg cggcggcggg cgaagacacc
     8461 atggtgtcgc tgtatcaggc gcacaagaag gtgtatcccc gcagcgtcac cggactgttt
     8521 gccaagtggc gctggttctt cgtttttgcg actcagattt tcttctacgg catgccgtgg
     8581 ctccagtggg gcgagcggca ggccatgttg ttcgatctgg aggcgcgacg cttctacatc
     8641 ttcgggctgg tgctctaccc gcaggacgtg atctacctga ccgcgatcct gatcatttcg
     8701 gcgctgtcgc tgtttctgtt cacggcggtg gccggacgcc agtggtgtgg ctacgcctgt
     8761 ccgcagactg tctacaccga gatctacctg tggttcgagc gcatgatcga gggcgaccgt
     8821 tcagcgcgca tgcggctgga cgcggcgccg atgtcggccg ccaagttcgg gcgcaaggcg
     8881 ctcaagcagg cggtgtggat cgctttcggc ctgtggacgg gctttacctt cgtcggctac
     8941 ttcacgccca tccgcgagtt ggcgcagcat gtgctgcagt ggaacctggg cgcatgggag
     9001 atcttctgga tcttcttcta cggctttgcc acctatggca acgccggctt catgcgcgag
     9061 caggtgtgca tgtacatgtg cccgtacgcg cgcttccaga gcgcgatgtt cgacaaggac
     9121 accctgatcg tcacctacga cgccaagcgt ggcgagccgc gcggcgggcg ctccaagagc
     9181 gccgatccca aggcgctggg attgggtgac tgcgtcaact gcacgttgtg tgtgcaggtt
     9241 tgtccgaccg ggatcgacat ccgcaacggt ctgcagtatg agtgcatcag ttgcgccgcc
     9301 tgcatcgacg tctgtgacga ggtgatggac aaggtcggct atccgcgtgg cctgatccgc
     9361 tactccaccg agcatgcact ggaacagggc tggggccgca aggagatcct gcgccaggtg
     9421 ctgcgtccgc gcgtgctgat ctacgtggcc atcctgtctg cggtggtggt cgcgctgatc
     9481 gccagtctgg tgctgcgcac accgttcaag gtggacatcg tgcgcgaccg cgccaccctg
     9541 gcgcgcctgg tcgatggcaa ccagatcgaa aacgtgtacc gcatccaggt gatgaacgcg
     9601 accgaaaagc cgcagacctt ccagctttcg gtggaagacc tgccgggtct ggaaatcgcc
     9661 tccgacaaga gcgtgaccgt cgacggcgcg cagagcctgt gggtgcctgt gcagctgcga
     9721 ctgccctacg gcagcgccag cagcggctct cacaccattc atttccgcgt gaccgcgccc
     9781 ggcgtcggcg aagtgcgcga aaagtccatc ttcatggtgc cgaggtgatg accatgtccg
     9841 accctgcttc taccccaccc agcccctggt ggaaattcgg ccacatgtgg ctggtcatcg
     9901 ccgggccgct gatcgtggtg ctggcctcca tcgtcacgat ctatctggcg gtccgcacgc
     9961 ccgacccggt gtatggggat gcgtccaggg gtgccgcccg atcgtcgcaa gccaaggatg
    10021 gcggcgacgc cggccgcatg tcgcccgcca tgcaggcgcg caaccatgcg gccaccgggg
    10081 gcgtcgccag accagcgccg aaccacggca ccggcccgaa ccagtgataa tgatccaggt
    10141 caaattcaaa ggggacaaac cgcgatgctg cgagagcgac tgatgtggat tgcgtggcca
    10201 gcgtttctga tggcggccgt gctggagttc atggtgtttg cggtgctcga cccgcaggcg
    10261 ctgacgctgc ttggcgaacc ctcgggctgg tcgcgcgaag gcgtctacac cgtcacgttc
    10321 ttcattttct gggcggtgat gatgctgtcc ggcgccctca ccaccctgct gtcgaagtcg
    10381 cccttcgacg tcaatcgccg ccagtcacgc gccctgcgct gatcgcgcgg tgcggccgat
    10441 cgggtcgggc gcccggggcg ccagcccatc ccacgcgaat cgacggctgg gctggaacag
    10501 gaatgggggg tgacgagcct tgaccccggc actgactaca aagttagggc tgcttggttc
    10561 cccacctgac ccggttggct ccttcaccat gcgggaaggc ccgtcgctgg ctattgtacg
    10621 gcttgcagac ggcgttcggc ttcggccaag ccgaaactca tggccaccag gttcagcacc
    10681 tcggcgcgca actggccaag ctgttcatcg tccgggtcgg ccgccagccg tgccttcagg
    10741 cgcgctgcgc gggggtggat gcagtctgtc agggcaccga ccaaggccgc gatgcgcgga
    10801 tcggcgtcgg tctgaatgac gatcgtgtcg tggctctcga aaacgtccat catggcgtac
    10861 agagtgaacc gtggcggggc aagccatcat ggagcaagcg cttgctgcat tgcagcatat
    10921 ccggtttctg gcccgtcggg tgcattccgt tgcatttgtg cgtcggtatt cgaggcgggt
    10981 ctctaattcg ggccatgctt ttcccgccac gtagaatcgc cgcatgtcgt atctggtgct
    11041 ggcccgcaaa taccggccgc ggaatttctc cgagatggtg gggcaggagc atgtcgtcca
    11101 ggccctcacc aacgcgctga cgcagcagcg cctgcatcac gcctacctgt tcaccggcac
    11161 gcgcggcgtg ggcaagacca cggtgtcgcg catcctggcc aagtcgctca actgcaccgg
    11221 cgccgacggg cagggcggca tcaccgccac gccctgcggc gtgtgcccgg cctgtgccga
    11281 catcgacgcc ggccgctttg tcgactacac cgaactggac gccgccagca accgcggcgt
    11341 cgacgaggtg cagcagctgc tggagcaggc cgtctacaag ccggtgcagg ggcgcttcaa
    11401 ggtcttcatg atcgacgaag tgcacatgct caccaacacc gccttcaacg ccatgctgaa
    11461 gacgctggag gagccgcccg aatacctcaa attcgtgctc gccaccaccg acccgcagaa
    11521 ggtgccggtg acggtgctgt cgcgctgcct gcagttcaac ctgcgcccca tggcgcccga
    11581 gaccgtgctg gaccacctga cgcgcgtgct ggcggccgag gcggtggcgg ccgagccggc
    11641 cgcgctgcgc ctgctggcgc gcgccgcacg tggctccatg cgtgacgcgc tcagcctgac
    11701 cgaccaggcc attgccttcg gctccggtga actgcgcgaa tccgcggtgc gccagatgct
    11761 gggcagcgtg gaccgcagcg tggtgttcag cctgatcggc gcgctggccg acggcgatgg
    11821 ccgcaccgtg gtgacgctca gcgagcagtt gcgcgccagt ggccactcgg cggcccagac
    11881 gctggaagac atggccagcg tgctgcagcg catggcggtg cagcaggccg tgcccgatct
    11941 tgcggtggac gcgtccgacc ccgacgcgca ggaaaccgcc cgcctggccg cgcgcctgcc
    12001 ggccgacgaa acgcagctgc tgtacagcct gtgcatccac ggccggtccg agctgggcct
    12061 ggcgccggac gaatataccg gtctgaccat ggcgctgctg cgcttgctgc cgtttcgcca
    12121 gccggtgccg acccaggccc agacggtcag cgccacggct gaaaaaaaaa gcctgaaaac
    12181 acctgaagcc tcgcccgcgc cgcagggcgc cagcgctccg cctgcgaagg cgcccgcgcc
    12241 agcgccggtc gcggcgagcg acgaccctgc tgcggcagag cagggtgcgg cagaaccggg
    12301 tgcggccgca gacgcccggc cgcaaccggt cacggaacgc gacgccgaac tggacacgca
    12361 gggcgcgcca cccgcccgcc cggtgcccgc gccgcgcccg cagccggcgc tgcagccgct
    12421 gccggtgcgg ccgtcgaacg gcacccgctt tcaagaacaa aatcaacctc cagcgcccga
    12481 ccatattgcg ccagtagctc ctgaaacaat agcaattcaa gtgcgtgaag gagaccagga
    12541 gccggcgccg gcgccgcgcc tgcgggtcga cgccccgccg ccgccggtgg tgcccggcgc
    12601 cgaaggcgac ttctggcacg cgctggtgca ggagctggtg gcgcgcgaag ccgtgacggc
    12661 cctggtgcgc gagctggcct tgcagtcgca gctggtggcg cgcaccgatc ggcagtggac
    12721 cttgcgggtg gaaagcgagt cgctggccca gtccggcgtg cgcgagcgcc tgcaggcggc
    12781 gctggccgac gccggccact gcgtgcgcct gcagatcgac tccggcccgg tcagcgacag
    12841 cccggcgcgt cgcaacgcca tccacgccac gcgccggctg aaggcggccg aggcgctgct
    12901 gctggccgac ccgttcgtgc aggagatgat gcgcgacttt ggggcgaaaa tcgtgccggg
    12961 cagcatcaaa ccgctctgaa tgaggcgcgc cgcagacgaa cccacccgcg cggtgcgcgg
    13021 cggtgctcac cgccggccga tggcgccacc gccgcctggg ccacagcccc ggaacacgca
    13081 ttgaagaaag gaagaaccga tgttcaacaa aggacaactc gccggcctga tgaagcaggc
    13141 ccaggccatg caggacaacc tgaagaaggc gcaggacgag ctggcgctga tggaggtcga
    13201 gggcgaatcc ggcgccggcc tggtgaaggt gctgatgacc gccaagcacg acgtcaagcg
    13261 cgtcaccatc gaccccagcc tgctggccga cgacaaggac atgctggaag acctggtggc
    13321 cgccgccttc aacgccgccg tgcgcaaggc cgaggagctg tcggccgaga agatgggcaa
    13381 gctgatgccc gccggcatgc ccgggttgcc ggggggcatg aagttcccgt tctgatgacg
    13441 aatgacggcg ctggcgcgcc atgaccttgc cgctccgcag ctgcgacgac atggggcatg
    13501 ggctcgaccg ccacggcggc gccgttgccg tcatcgagcc gcaaccaggc gaggtcactt
    13561 cgtcaggggg catgccagca tgagcgacac ccacagcctc gacaccctga tccaggccct
    13621 gcggcgcctg ccgggcgtgg gccagcgctc ggcgcagcgc atggcgtttc acctgctgca
    13681 gcacgatcgc gaaggcgcgc agcagctggc gcgggcgctg cacgaggcgg tcggtcaggt
    13741 gcgtcactgc gcccgctgcc acaccttcac cgaggccgag gtgtgcgcca cctgcagcga
    13801 cccgcggcgc gacgccagcc agctggcggt ggtcgagacc ccggccgacc aggcggcgct
    13861 ggagcgcacc ggggccttcc gcgggcgcta cttcgtgctg atgggccggc tcagcccgct
    13921 ggacggcatc ggcccgcgcg acatcggcgt gcagcggctg atcgagcgcg cctgcgacgg
    13981 cgtggtgcgc gaagtgatcc tggccaccaa cttcaccgcc gagggcgagg ccacggcgca
    14041 cctgatcggg caggcgctgc gcagccgcgg cctggccgtg acgcggctgg cgcgcggcgt
    14101 gccggccggc agcgagctcg aatacgtgga cctgggcacg atcgcccacg cgctggtcga
    14161 ccggcgctga accgccatgt cgctgtccgc gtcgatcctg gctgcccgcc agctgctgca
    14221 ggaggcggcg cacatcgccg tgctcagtgg cgccggcatc agcgccgagt ccggtgtgcc
    14281 caccttccgc gacgcccaga ccggcctgtg ggcgcacttg cgcccggagg atctggccac
    14341 cgaagacgcc tttcggcgcc accctcagcg cgtgtgggac tggtacgccg agcgccgcgc
    14401 cagcctggcc gaggtgcaac ccaacgccgg ccaccgggcg ctggccgaat ttcagcaccg
    14461 ccacccgggt cggctgacgg tgatcacgca gaacgtcgac ggcctgcacc agcgcgccgg
    14521 cagcaccggc gtgctggcgc tgcatggcag cctggcggcc gaccgctggc tcgatccctg
    14581 cccggcgggg cgcggcccga ccgcctgcga tgtggcgcgg gccgaccctg gccgcccccc
    14641 gcgctgcgcc cagtgtggca actgggtgcg cccggcggtg gtctggttcg gcgagctgct
    14701 gcccgaagac cagctgcggc aggccgaaca ggccgccagc cgctgcgacg ccatgctggt
    14761 ggtcggcacc gccggcgcgg tgtatccggc ggccgggctg gcgcaccagg cgcgtgccgc
    14821 cggggcgcgt ctgatcgtcg tcaatcccca ccccagcgag ctggacgggc tggcccagct
    14881 gcggctggtc ggcggcgcgg cccaggtgct gccgcccttg ctggactttt gaccacgcgg
    14941 ccgctgcggc agcgccctgc ggtgccgcag gtgcctcgtg aattgctatt gatgcggtag
    15001 ctgctggcgc atgcctggtg ctgcctcagg ccggatttga tgctccatcg gcgcggcccg
    15061 cccggggccg cgcgctcagg tgctggccca catgcgcagc aggttgtggt aggtgccggt
    15121 caggcggatc gcctcgtcgg tttcgccgtg gcgctggcgc agcgccagca gcgccatgtc
    15181 catctcgaac agcaggcggc gctgctcctg gccgcgcacc atggattcga tccacagaaa
    15241 gctggcgatg cgcgcgcccc gggtgaccgg gcgcacctcg tgcacgctgg tgccggggta
    15301 gagcacggcg tcgcccgccg gcagcttgac ggcctgcggg ccgaaggtgt cgtgcaccac
    15361 cagctcgccg ccgtcgtagt cgtcgggctc ggtcaggaac acggtgcacg aaaggtcgct
    15421 gcgcacccat tcgccgctgg tctgtgagtg cagcacggcg ccatccacat gctggccgta
    15481 gtggttggcc acgccttcgt agcggttgaa cagcgggttg aagatcctgc gcggcagcgc
    15541 cgccgagaag aacaccgggt cgcgccgcag cgccgccagc accagctcgc gcagcgtggc
    15601 cgcgctgggg ctgtcctgcg ccagctggcg gttgctcttg cgctgcacgg cctggccgcc
    15661 ggcgctgcgc gcgccatcga cccagggtgc gtcgtcggcc agcagcagac ggcgcgcgtg
    15721 cgccacttcg tcggtgctga gcaggttctt gaggtgaatc aacatgggag attccgccaa
    15781 agacgacagc cgcccgaggg cggctgtgca ggttggatgt ggtggtcagt ccatcagaac
    15841 aggtgcttga gcgtcagctc cacccggcgt ggcgcgcccg gttggtagaa gccccggtac
    15901 agcccgtcgg cgtacagctt gttgctcagg ttggtgacgc tgagcttgag cgtggtggct
    15961 tcggtgaagc tgtattcggc catggcgtcg aaggtgacga agcccttggc cttcagggtg
    16021 cgctgacctt ccgggttctg gctgctgcgg tagttggcgc ccagccccag gcgcagttgc
    16081 ggcatcacgc ggtaggtggt ccagacgctg ccgctgtggc gcggcgtcag gcccgggcgg
    16141 tcgccctgca cctgcgcgcc ggtgccggcg gggttcagca ccacgttgct cttgtcgatc
    16201 ttggctttcg ggatccaggt gtggttccag aacagttccc aggccggcgt gatgcggccc
    16261 gccaggttga attccatgcc ggcggcgtgg cgcttgccgg acagcagctc ctgcgtggcg
    16321 gcggtgtcgg ggtcggtgtt gcgctcgttg tacttctcgc tgtagaacag ggcggcgccg
    16381 gccagcatgc gcttgtcgaa caggtcccac ttgccgccga tttcgatgtt gcggctcttt
    16441 tcgggcgggg tcttggccga gcggccggtg ttggggttga tcagcacgcc gtactgatag
    16501 gcgtcgcccg aggtgttgaa cgaggtgccg taggagatgt agtaggacga cgcctcgtcg
    16561 ggctggtaga gcagacccag gcgcgggctc cacaggttgt cggtgcgcgc gttgctgagc
    16621 gagccgtcgg cgttgcggta cgagcccttg aagtggtcga agcgcaggcc gccgaccagc
    16681 ttgacggtgt cggtgagcga gacggtgtcc tgcaggt
//
LOCUS       DAOOOG010000036        16402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_3731_length_16402_cov_22.066495, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000036 DAOOOG010000000
VERSION     DAOOOG010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 16402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16402
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3731_length_16402_cov_22.066495"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..716
                     /locus_tag="PK242_04655"
     CDS             237..716
                     /locus_tag="PK242_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HOZ93339.1"
                     /translation="MTKGSRIVLLALACTACTGALAQKIGDVSTAFQWIGPNHKIVVE
                     AYDDPLVQGVTCYVSRARTGGIKGGLGLAEDRAEASIACRQVAPVQFKEPLRREDEVF
                     SERMSILFKRLRVVRMVDAPRNTLVYLTYSDKLIEGSPQNAITAVPIDHATPIPLRP"
     gene            complement(862..957)
                     /locus_tag="PK242_04660"
     tRNA            complement(862..957)
                     /locus_tag="PK242_04660"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(921..923),aa:Sec,seq:tca)
     gene            complement(964..2865)
                     /gene="selB"
                     /locus_tag="PK242_04665"
     CDS             complement(964..2865)
                     /gene="selB"
                     /locus_tag="PK242_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOZ93340.1"
                     /translation="MIVGTAGHIDHGKTTLVRALTGVDTDRLPEEKKRGISIELGYAF
                     LEPPSGARIGFIDVPGHERLVHTMVAGASGIDFALLLVAADDGPMPQTREHLAVLSLL
                     GLTRGAVVITKADRATPERVQAVREQTRALLVGTTLESVPVLAVSAHTGAGIDDLRAL
                     LFVAAGAEQASAEAEFDSKALRLAIDRAFTLQGTGTVVTGTIHAGSVGVGDELAIVPG
                     TLRARVRSLHAQNQAVARAHAGQRCAVALAGLARDEVTRGQWLTAPAVALASERLDLR
                     LTLWPGEAQALRSGTRVQAHLGAAHVNATVALLDSDALAPGATALAQLLLQAPVGAWH
                     SERVLLRDAAASRTLAGGRVLDPLAPARYRRTPQRLAELSALGLPSAAERLTALLAVA
                     PHGVDLKRFAAAQGLAHPPDQALPAPALVQDGLALGADQAHTLAAAALAALQRFHDQQ
                     PDELGPDSARLRRLSAPRLPAPLWQGVLARLATEGQLVLHGAVVHLPSHGAQLSATEA
                     RIAQKVAPALVQAGFQGAWARDLARDAAEPEPLVRTTLARLARRGELHQIVKDLYYPP
                     DTLQRLAALARQVGAAHQGIVRAADFRDATDLGRKRAIQILEYFDRIGFTRRIGDEHR
                     LRTDCALFS"
     gene            complement(2879..4273)
                     /locus_tag="PK242_04670"
     CDS             complement(2879..4273)
                     /locus_tag="PK242_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ93341.1"
                     /translation="MTIDRRHFVAGLAGCAALAGCAAAPPVSGVGVQTAPMPAFIGQS
                     TQTLLPRGNAPRVLVVGGGWGGLTTAANLRKQAPGAEVVLIDRNPQFFSSPLSNMWLV
                     GMVDGELLTQDYLRVSEQLGYRFVQAEVQAIDRAQRRVTTSLGTIDYDWLVLAPGIRY
                     QYEAWFGNDRVAANAARTRFPCAYASNSEFLAIKRSLQDFKGGELVMNLPPPPQRCPP
                     SPYERACLIAWYFKTNKIKGHITILDHKPAITPIGPGYRAAFDELYKDYITYVPNAHV
                     QEVDPFNRRIRTRAGDQRFDHAILMAPHKAGDLAWLAGTVSDRSEGWADVDQVTMQVR
                     GDERTFVIGDAIQAVSSLPFRYYPKSAHIANRLGRIVAYQISERLAGRAPERRLPDNL
                     CYMVVNGTPGQALNVQFDYDFDPEGRVRQVQRDFNERTPALFQDDFRWLDFMFGEMFG
                     GIAPPVRPNPAFRT"
     gene            complement(4278..5765)
                     /gene="selA"
                     /locus_tag="PK242_04675"
     CDS             complement(4278..5765)
                     /gene="selA"
                     /locus_tag="PK242_04675"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOZ93342.1"
                     /translation="MTAPEQSVVHGTPAPPDAPTTAAPHALPALDRLLRLPAVAALEA
                     EHGRSLLTHEARALLAELRQQALAGQLPTAEIAPDALAGALARRLHDRLAPRLNTVIN
                     LTGTVIHTNLGRALLPEAALAHVQATMAGPNNLEYDLASGGRGDRDSIVEGLLCQLTG
                     AEAATVVNNNAAAVLLILAALARGRQVIVSRGELVEIGGAFRMPDVMTSAGARLVEVG
                     TTNRTHPHDYERAITARTALLMKVHTSNYAVQGFTRAVDEATLAPIAHAHGLPLVTDL
                     GSGALIDMTAYGLPAEPTPQQMLRAGCDLVSFSGDKLLGGPQAGLIVGRRALVERVRK
                     HPMKRALRMSKLPLAALEATLRLYLRPERLARELPTLRLLTRPLADIEATARAVQPAL
                     AAALAPRFTVETVALQGQIGSGSLPVERLPSAGLAIAPVARTGRGRALDELATALRRL
                     PVPVIGRIADDQLILDCRCLEDSTLLTRQLRQLSPQTTTVPPATK"
     gene            complement(5765..6757)
                     /gene="fdhE"
                     /locus_tag="PK242_04680"
     CDS             complement(5765..6757)
                     /gene="fdhE"
                     /locus_tag="PK242_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HOZ93343.1"
                     /translation="MTTTHPTTLPSPEEIALQAGADVAAVRAPQADTLFAERALRLRE
                     LAAGHAMRDYLLLLALVSEAQHERARRYPQVRLPDAAQIDASAATGQPLLAATAWPRE
                     PLWRTELRALLAQVLQQLPGDSPARATVQAVIGLPDQALEQQADRLLAGITLGLDMAA
                     APLIAAGLQLYWAHLVAGTASAHPGLWRHLESRGTCPCCGSLPTSSIRRVGPGMKGQR
                     YLHCTLCQTEWHMVQVMCTHCLGTQGIHYQALQALAAAAPTSERAAVEAETCDHCHHY
                     LKLLHMEREPHADPVADDLATLTLDLLVSDAGFTRHGTNLLLLFGDAEAPPGER"
     gene            complement(6830..7483)
                     /locus_tag="PK242_04685"
     CDS             complement(6830..7483)
                     /locus_tag="PK242_04685"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HOZ93344.1"
                     /translation="MKPMYLQRYSAGQRSNHWAVVACFVLAAVSGFALFHPALFWLSA
                     LVGGPQWTRILHPFLGAAMCLLFLGMFFMLAGANVWRSEDSQWLGSAGKLVSSEGGHA
                     DMPPVGKYNGGQKLVFWLFALSLLVLLISGLVVVWPQWFGGGASIGMQRMAIVLHAVA
                     AVVLVLAVIVHAYAAFWVKGTLRAMTRGSVSASWARHHHPLWYRERMQNPSSDARVK"
     gene            complement(7480..8388)
                     /gene="fdxH"
                     /locus_tag="PK242_04690"
     CDS             complement(7480..8388)
                     /gene="fdxH"
                     /locus_tag="PK242_04690"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HOZ93345.1"
                     /translation="MSSLQSQDVVARSATTTPPPQARSSTPQVAKLIDVSKCIGCKAC
                     QVACMEWNDLRDEVGSNIGVYDNPTNLTAQSWTVMRFYEAEPEQDKLEWLIRKDGCMH
                     CEDPGCLKACPSPGAIVKYANGIVDFISEHCIGCGYCIKGCPFDVPRISPQDNKAYKC
                     TLCSDRVGVGLEPACVKTCPTGAISFGTKDDMVSYGQQRAADLKERGHQNAGLYNPPG
                     VGGTHVMYVLKHADRPELAGLPKEPSISPWVSVWKGWTKPVAMAAMAGAALAGWFHYL
                     RKGPLEVPEDAEATAGGATRTEGESS"
     gene            complement(8392..11472)
                     /gene="fdnG"
                     /locus_tag="PK242_04695"
     CDS             complement(8392..11472)
                     /gene="fdnG"
                     /locus_tag="PK242_04695"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016497753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(10885..10887),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HOZ93346.1"
                     /translation="MDMNRRQFFRVSGAGLAASSLVALGFSPTAALAETRAFKLTRAS
                     VSRSTCPYCSVSCGMLIYKLGDTAKNVREHVIHVEGDPDNPVNRGTLCPKGAGVMDMI
                     NSPGRVIYPSVREAGSKEWKRISWDEALTRVARHMKADRDANFQAKNDAGTTVNRWPT
                     TGFLVCSSSSNESSYLSVKVARALGMVGIDTQARVUHAPTVTSLGPTFGRGAMTNSWT
                     DIRNTDLILVMGGNAAEAHPVGFRWAVDAMEKRKARLISVDPRFNRTSSVADLYAPLR
                     NGTDIAFLGGVINWLLSHDKIHHDYVKFNTDATFLLKPDFKLENGIFSGYDEAKRKYD
                     KASWGYQLDENGHVKVDDSLQDPLCVYQQLKKHYSRYTPEMVSRICGTPQDKFLKVCE
                     MLGEMSAPDKTSTILYALGWTQHSVGSQNIRTMAMIQLLLGNMGRPGGGVNALRGHSN
                     VQGVTDMNAYAEVLSGYLSAPTDADDTFEKYLAKRTPKPMRPNQFNYWSNFPRFHISL
                     MKSYYGKAATADNQYCYDWVPKLAGGFDALHMFELMHQGKMTGFISQGFNPLATVPNK
                     NKLSAALSKLKYLVIIDPLETETGEFWQNHGPFNDVKPESIQTEVFRLPSACFAEEEG
                     SFTNSSRIAIWKERALEPPGEAKVDSEIMARLFLKLRQMYAKDGGAFPDAIAGLAWDY
                     VQPEAPSATEVLREINGKALSDVLAPPDPNNPTAPRQVLVKAGQQVPGFAMLRDDGST
                     ACGNWLYAGCWSEAGNLTARRDVSDPTGLGVYQNWGFSWPANRRILYNRASADKNGKP
                     WSENKKYVYWNGKAWAGADVPDMRPNAAPEENVGSFIMNPEGVARLHAIGLADGPFPE
                     HYEPFETPIGVNPLHPDNPKAISNPAARVFKGDLEAFGKKEEFPYSATTYRLTEHFHT
                     WTKHARLNAIVQPESFVEISEELAKEKNIRHGDQVKVSSNRGHIVTRAVVTKRIKPWN
                     VNDQKVHLVGIPFHWGFKGQTKNGYLANTLSPFVGDANSQTPEFKAFLVNIEKV"
     gene            complement(11651..13480)
                     /locus_tag="PK242_04700"
     CDS             complement(11651..13480)
                     /locus_tag="PK242_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HOZ93347.1"
                     /translation="MALPHRPTTLAALAAATLLAGCASSLNYTPPATPVTPEGGSGWT
                     DKPGWATHKFAVAAANPLATDAGYQVLKAGGSALDAAIAVQMVLTLVEPQSSGIGGGA
                     FLLHAAGGDVTAWDGRETAPAAVDDKLFLGADGKPLPFHDGVVGGRSVGVPGTVRMLE
                     QAHRQHGRLAWAELFKPAIVLAEQGFAVSPRLHTLLKNEQHLKKDPVAARHFYDAAGN
                     PWPVGHVLKNPELAAVLKGIAARGSTALLEGPVAQAIVDKVQKHPTNPGKLTLADLAG
                     YTPKRRAPLCHELRVPASGKAYEVCGFPPPSSGAIAVGQILGILAHTPAASMAAGADG
                     LPTADWLHYYMEASRLAFADRALYVADPDFVQPPAGSWMSLLDPAYLKSRAALIGTQS
                     LKVAPPGKPVGTRTSFAPMASQPEYGTSHISIVDPFGNALAMTTTIEDQFGARQMVKG
                     FLLNNELTDFSFAPTDAQGQPIANRVQPGKRPRSSMAPTLVFEKTTDGRRGPLVMSAG
                     SPGGALIIHYTAKTLHGVLGWGLLPQQAINLPNFGSLNGPSLLEEKKFPAATVEALRS
                     RGAEVREMNLTSGLQAITRGTVHGQPTWLGGADPRREGVVMGD"
     gene            complement(13670..14428)
                     /locus_tag="PK242_04705"
     CDS             complement(13670..14428)
                     /locus_tag="PK242_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOZ93348.1"
                     /translation="MQIKDKVFIVTGGASGLGEGTARMLAARGGKVVIADMQVDKGEA
                     VAREIGGAFVKCDVSSEADGQAVVAKAVSMGKLMGLVNCAGIATAERTVGKNGPHRLD
                     GYTRTIMVNLVGTFNMIRLAADAMSRNEPEPTGERGVLISTASVAAYDGQIGQAAYSA
                     SKGGIVGMTLPIARDLARTGIRNMTIAPGIMGTPMLFGMPKEVQDALAAGVPFPSRLG
                     TPEDYAKLACHIIENDMLNGEVIRLDGAIRLAPK"
     gene            14555..14878
                     /locus_tag="PK242_04710"
     CDS             14555..14878
                     /locus_tag="PK242_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93349.1"
                     /translation="MCIHCSPHDRRDDDELRPTAEALLAATLALMTGVAEAAPGAAGV
                     RPMSLKVLANLDELARHPGLSRSMRQLAAHLGLHWQQLVQTARGEARAAGERARWVPA
                     STVVQ"
     gene            14875..15291
                     /locus_tag="PK242_04715"
     CDS             14875..15291
                     /locus_tag="PK242_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93350.1"
                     /translation="MSALEPGALQREHQLLLRHLGRVQQRVSEQMLAQAQRCAALEAE
                     LMQWRARWIVATTRRLWGLGGFDQPPAPARAVPADRSAWASATEVLCQTGCAGHAHPW
                     RDEQGRCALTASDCTRVPPVAQDASAGAVARVFSRP"
     gene            complement(15253..16344)
                     /locus_tag="PK242_04720"
     CDS             complement(15253..16344)
                     /locus_tag="PK242_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOZ93351.1"
                     /translation="MNPHRPAPTLDTDVAIIGAGPVGLFAVFQLGLLELRAHVIDALP
                     HAGGQPVELYADKPIYDIAAVPVCSGQTLTDALLRQIAPFAPQWHLGHLVESLQRLPD
                     GRFLLQTEPGRRLRARSVFIAAGVGAFLPRRLKVAGLEAFERRQLFHQAPADAALAGQ
                     HVVVVGGGEPALTQAIRLATLAAAAPASVTLLHRRDGFQAAPATQARVRALCAAGSMR
                     LRVGQVVGFEQAAGRLSALQVMGPDGSTQPLPLELLLVQQGVSPRLGPITAWQLALER
                     KQLVVDAATFQTSEPGIFAIGDINTYPGKKKLMVCGFHEATLAAYAAREHIHPHDPLP
                     FEYTTSSARLQRLLGMASGAREHAGDGAG"
BASE COUNT         2339 a         5435 c         5946 g         2682 t
ORIGIN      
        1 tgtagatctc gcccgacagc cggatgccca gctcggtgat gcccacggtg gccgtaccgt
       61 cgccggccag acgggcccac acctggtgct ccatcaggta gtgcaggtcg gcagggtgct
      121 gcagcggcgc gttcgacatg aggggtattc tggcaggccc tgggacacta ttaaacctat
      181 agctgctggc gcaagctaca cttgcgccaa cggcattttt tattggaatt gaagcgatga
      241 ccaagggttc tcggatcgtg ctgctggcgc tggcttgcac cgcctgcacc ggcgcgctgg
      301 cgcagaagat cggcgacgtc agcaccgcct tccagtggat cggccccaac cacaagatcg
      361 tcgtcgaggc ctacgacgac ccgctggtgc agggggtgac ctgctacgtt tcgcgcgcgc
      421 gcaccggcgg catcaagggc gggctggggc tggccgaaga ccgtgccgag gcttccatcg
      481 cctgtcgcca ggtggcgccg gtgcagttca aggagccgct gcggcgcgag gacgaggtgt
      541 tcagcgaacg catgtcgatc ctgttcaagc gcctgcgcgt ggtgcgcatg gtggatgcgc
      601 cgcgcaacac gctggtgtat ctgacctact ccgacaagct gatcgaaggc tcgccgcaga
      661 acgccatcac ggcggtgccg atcgatcacg ccacaccgat cccgctgcgg ccttgatgcc
      721 gggtgcgcct tcggtacctg ccgtatccag gatgaccgcg cggcgcaccg cggacgcatc
      781 ggtcatgcgg cagcgccggt gatcccaggc gcggcctgaa gcgcgtggtg tggcggtggg
      841 cggccccgtc ggacgccggg gtggcggaag ggcatgggag tcgaacccac ccggcggcgc
      901 ttggcgccac ccaccgggtt tgaagcccgg cccacccacc aggatggttg cccttccgtt
      961 gacctacgaa aacagcgcgc agtccgtgcg caatcggtgc tcgtcgccga tgcggcgcgt
     1021 gaaaccgatg cggtcgaagt attccagaat ctgaatcgcc cgcttgcggc ccaggtcggt
     1081 cgcgtcgcgg aagtcggccg cccgcacgat gccctggtgt gcggcgccca cctggcgcgc
     1141 cagggccgcc agccgctgca gggtgtcggg cgggtaatac aggtccttga cgatctggtg
     1201 cagctcgccg cggcgcgcca gccgcgccag cgtggtgcgc accagcggct cgggctcggc
     1261 ggcgtcgcgc gccagatcgc gcgcccaggc gccctgaaag ccggcctgca ccagcgccgg
     1321 cgccaccttc tgtgcaatgc gcgcttcggt ggccgacagc tgcgcgccgt ggctgggcag
     1381 gtgcaccacc gccccgtgca gcaccagttg cccttcggtc gccagacgcg ccagcacgcc
     1441 ctgccacaag ggggctggca ggcgcggtgc gctcaggcgg cgcaggcggg cgctgtcggg
     1501 gcccagctcg tcgggctgct ggtcgtgaaa acgctgcagc gccgccagcg cagcggccgc
     1561 cagcgtgtgc gcctggtcgg cgcccagggc caggccgtcc tgcaccagcg ccggcgccgg
     1621 cagcgcctgg tcgggcgggt gggccaggcc ctgggcggcg gcaaagcgct tcagatcgac
     1681 gccgtgcggc gccaccgcca gcagcgccgt caggcgctcg gcagcgctcg gcagacccag
     1741 ggcgctcagc tcggccagcc gctgcggcgt gcgccggtag cgggcggggg ccagggggtc
     1801 gagcacgcga ccgcccgcca gcgtgcggct ggctgcggca tcgcgcagca gcacgcgttc
     1861 gctatgccag gcgcccaccg gggcctgcag cagcagctgt gccagggcgg tggcgccggg
     1921 cgccagtgcg tcgctgtcca gcagagccac ggtggcgttg acgtgggccg cgcccaggtg
     1981 ggcctgcacc cgggtgcccg agcgcagcgc ctgggcttcg cccggccaca gcgtcaggcg
     2041 caggtcgagg cgctcgctgg ccagcgccac ggcgggggcg gtcagccatt gaccgcgcgt
     2101 cacctcgtcg cgcgccagcc cggccagggc cacggcacag cgctggccgg cgtgggcgcg
     2161 tgccaccgcc tggttctgcg cgtgcaggct gcgcacacgg gcgcgcaagg tgccaggcac
     2221 gatcgccagc tcgtcgccca cccccaccga gccggcgtgg atggtgccgg tgaccaccgt
     2281 gccggtgccc tgcagggtga aggcgcggtc gatcgccagg cgcagcgctt tggagtcaaa
     2341 ttcggcctcg gcgcttgcct gttctgcgcc agcagctaca aaaagaagag ctcgcaggtc
     2401 gtcgatgccg gccccggtgt gggccgacac ggccagcacc gggacgcttt cgagcgtcgt
     2461 gccgaccagc agcgcgcgcg tctgctcgcg caccgcctgc acgcgctcgg gcgtggcgcg
     2521 gtcggccttg gtgatgacga cggcgccgcg cgtcaggccc agcaggctga gcacggccag
     2581 gtgttcacgc gtctgcggca tggggccgtc gtcggccgcc accagcagca gcgcgaagtc
     2641 gatgccgctg gcgccggcca ccatggtgtg caccagccgc tcgtggcctg gcacgtcgat
     2701 gaagccgatg cgcgcgccgc tcggtggttc gaggaaggcg tagcccagct cgatgctgat
     2761 gccgcgcttt ttttcttcgg gcaggcggtc ggtgtccacg ccggtgagcg cgcgcaccag
     2821 cgtggtcttg ccgtggtcga tgtgaccggc ggtgccgacg atcatgagct cgccgcagtc
     2881 aggtgcggaa ggccgggttg ggccgcaccg gcggcgctat gccgccaaac atctctccga
     2941 acatgaagtc cagccaacgg aagtcgtcct gaaacagggc cggcgttcgt tcgttgaagt
     3001 cgcgctgtac ttggcgcacg cgaccttcag ggtcgaagtc gtagtcgaac tgaacgttga
     3061 gcgcttgccc cggcgtgcca ttgaccacca tgtagcacag gttgtcgggc aggcgccgct
     3121 cgggcgcgcg acctgccagt cgttcgctga tctggtaggc gacgatgcga cccaggcggt
     3181 tggcaatgtg ggcactcttg gggtagtagc ggaagggcag agacgaaacc gcctgaatgg
     3241 cgtcaccgat gacgaaggtg cgctcgtcgc cccggacctg catggtcacc tgatcgacgt
     3301 cggcccagcc ctcgctgcgg tcgctgacgg tgcctgccag ccaggcgagg tcgccggcct
     3361 tgtgcggtgc catcaggatg gcatggtcga agcgctgatc accggcgcgg gtacggatgc
     3421 ggcggttgaa tgggtccacc tcctgcacgt gtgcgttggg cacataggtg atgtagtcct
     3481 tgtacagctc gtcgaacgcg gctcggtagc cgggtccgat gggggtgatg gcgggcttgt
     3541 ggtcgaggat ggtgatgtgg cccttgatct tgttcgtctt gaagtaccag gcgatcaggc
     3601 aggcgcgctc gtaggggctg ggtgggcagc gttgcggggg aggcggcagg ttcatcacca
     3661 gctcgccccc tttgaagtcc tgcagcgatc gcttgatggc caggaattcg ctgttgctgg
     3721 cataggcgca gggaaagcgc gtgcgtgcgg cgttggccgc cacgcggtcg ttgccgaacc
     3781 aggcttcgta ttggtaacgg atgcccggtg ccagcaccag ccagtcgtag tcgatcgtcc
     3841 ccaggctggt ggtcacacga cgctgcgcgc ggtcgatggc ctgaacctcg gcctgcacga
     3901 accgatagcc cagctgctcc gacacacgca ggtagtcctg cgtgagcagt tcgccatcca
     3961 ccatgccgac cagccacatg ttcgacaacg ggctggagaa gaattgcggg ttgcgatcga
     4021 tcaaaaccac ttctgcgcct ggcgcctgct tgcgcaggtt ggccgccgtg gtcagaccgc
     4081 cccagccgcc gcccacgacc agcacgcgcg gcgcgttgcc tctgggaagc aaggtctggg
     4141 tcgactggcc gatgaacgcg ggcatcggcg cggtttgaac gccaacgccg gataccggag
     4201 gtgcggcggc acagcccgcc agtgcggcgc agccagccag gccggcgacg aagtggcgac
     4261 gatcgatggt catgggttta cttggttgca ggcggaacgg tcgttgtttg cggagacaac
     4321 tgccgcagct ggcgggtcag cagggtgctg tcttccaggc agcggcaatc cagaatgagc
     4381 tggtcgtcgg caatgcggcc gatgaccggc accggaagcc gacgcagggc cgtggcaagc
     4441 tcgtccagtg cgcgaccgcg gccggtcctg gccaccggcg cgatggccag gccggccgag
     4501 ggcaggcgct cgaccggcag cgagccggag ccgatctgtc cttgcagcgc caccgtctcg
     4561 accgtgaagc gcggcgccag cgccgctgcc agcgccggct gcaccgcgcg ggcggtggct
     4621 tcgatgtcgg ccagcgggcg cgtgagcagg cgcagcgtgg gcagctcgcg cgccagccgc
     4681 tcggggcgca ggtacaggcg cagcgtggct tccagggcgg ccagcggcag cttgctcatg
     4741 cgcagggcgc gcttcatggg gtgcttgcgc acgcgctcga ccagcgcccg gcggccgacg
     4801 atcaggccgg cctgcgggcc gcccagcagc ttgtcgccgc tgaagctgac cagatcgcag
     4861 ccggcgcgca gcatctgctg cggcgtcggc tcggccggca gaccgtaggc cgtcatgtcg
     4921 atcagggcgc cgctgcccag gtcggtcacc aggggcaggc cgtgggcgtg cgcgatcggg
     4981 gccagcgtgg cttcgtcgac cgcgcgggtg aagccctgca ccgcatagtt gctggtgtgc
     5041 accttcatca gcagagcggt gcgcgcggtg atggcgcgct cgtagtcgtg cgggtgggtg
     5101 cggttggtgg tgccgacctc caccagccgc gcgccggcgc tggtcatgac gtcgggcatg
     5161 cggaaggcgc cgccgatctc caccagttca ccgcgcgaga cgatcacctg ccgcccgcgc
     5221 gccagcgcgg ccaggatcag cagcaccgcg gcggcgttgt tgttgaccac ggtggcggcc
     5281 tcggcgccag tcagctggca caacagaccc tcgacgatgc tgtcgcggtc gccccggccg
     5341 ccgctggcca ggtcgtattc gaggttgttg ggcccggcca tcgtggcctg cacgtgggcc
     5401 agcgcggctt cgggcagcag ggcgcggccg aggttggtgt ggatcaccgt gccggtcagg
     5461 ttgatcacgg tgttcaggcg cggcgccagg cggtcgtgca ggcggcgcgc cagggcgccc
     5521 gccagggcgt cgggggcgat ttcggcggtc ggcaactggc cggccagggc ctgctggcgc
     5581 agttcggcca gcagggcgcg tgcctcgtgc gtgagcaggc tgcggccatg ttcggcctcc
     5641 agcgcagcaa ccgccggcag gcgcagcagg cggtcgaggg cgggcagggc gtggggggcg
     5701 gcggtggtcg gggcgtctgg cggggccggc gtaccgtgaa ccacggattg ctcaggcgcc
     5761 gtcatcagcg ctccccgggc ggcgcctcgg cgtcaccgaa cagcagcagc aggttggtgc
     5821 cgtggcgcgt gaagccggca tcggacacca gcagatcgag cgtcagtgtg gccaggtcgt
     5881 cggccaccgg gtcggcgtgc ggctcgcgct ccatgtgcag cagcttcagg tagtggtgac
     5941 agtgatcgca ggtttcggct tcgaccgcag cccgttctga cgtcggtgcg gccgcggcca
     6001 gcgcctgcag cgcctggtag tggatgccct gggtgcccag gcagtgcgtg cacatgacct
     6061 gcaccatgtg ccattcggtc tggcacaggg tgcagtgcag gtagcgctgc cccttcatgc
     6121 ccgggccgac gcgccggatg ctgctggtgg gcaggctgcc gcagcagggg caggtgccgc
     6181 ggctctccag gtgtcgccac agccccgggt gggcgctcgc ggtgcctgcc accagatgcg
     6241 cccagtacag ctgcaggccg gcggcgatca gcggcgccgc cgccatgtcc agccccagcg
     6301 tgatgccggc cagcaggcga tcggcctgtt gctccagcgc ctgatccggc aggccgatca
     6361 cggcctggac cgtggcgcgg gccgggctgt cgcccggcag ctgctgcagc acctgggcca
     6421 gcagcgcgcg cagctcggtg cgccacagcg gctcgcgcgg ccaggcggtg gccgccagca
     6481 gcggctggcc ggtggcggcg ctggcatcga tctgcgccgc gtcgggcagc cttacctgcg
     6541 ggtagcggcg cgcgcgctcg tgctgcgcct cgctcactag cgcgagcaac agcaggtagt
     6601 cgcgcatggc atggccggcg gccagctcgc gcaggcgcag cgcccgttcg gcaaacagcg
     6661 tgtcggcctg cggcgcgcgc acggcggcta cgtcggcgcc ggcctgcaga gcgatctctt
     6721 cgggcgaggg cagagtggtg ggatgggtgg tggtcaaggc aaaaaagaac tgccgccggc
     6781 gcaccgcacc ggcaggcagt gggggtgggt gggggaaatc aggacaacct catttgacgc
     6841 gcgcgtcgga cgatgggttc tgcatgcgtt cacggtacca cagcggatgg tggtgccgag
     6901 cccagctggc gctgacgctg ccgcgcgtca tggcgcgcag cgtgcccttg acccagaagg
     6961 cggcgtaggc gtgcacgatc acggccagca ccagcaccac ggcggcgacg gcgtgcagca
     7021 cgatcgccat gcgttgcatg ccgatcgatg cgccgccgcc gaaccactgc ggccagacca
     7081 ccaccagacc gctgatcagc agcaccagca ggctcagcgc gaacagccag aacaccagct
     7141 tctgcccgcc gttgtatttg cccaccggag gcatgtcggc gtgtccgcct tcggaagaca
     7201 ccagtttgcc ggccgatccc agccattgcg agtcttcgct gcgccagacg ttggcgccag
     7261 ccagcatgaa gaacatgccc agaaacagca ggcacatggc ggcacccagg aaggggtgca
     7321 ggatgcgcgt ccactgcggg ccgccgacca gggcgctcag ccagaacagc gccggatgga
     7381 acagcgcgaa gccggacacg gcggccagca cgaagcaggc caccaccgcc cagtggttgg
     7441 atcgctggcc ggcgctgtag cgttgcaggt acatcggctt catgacgact ctccttccgt
     7501 gcgggtcgcg ccacccgcgg tggcttcagc gtcctcgggg acttccagcg ggcccttgcg
     7561 caggtagtgg aaccagcccg ccagggcggc gccggccatc gcggccatgg cgaccggctt
     7621 ggtccagccc ttccagaccg acacccacgg gctgatgctc ggctccttgg gcaggccggc
     7681 gagctcgggc cggtcggcgt gcttgagcac gtacatgacg tgcgtgccgc cgacacccgg
     7741 cgggttgtac agcccggcgt tctgatggcc gcgctccttc aggtcggcgg cgcgctgctg
     7801 gccgtagctc accatgtcgt ccttggtgcc gaagctgatg gcgccggtcg ggcaggtctt
     7861 gacgcaagcc ggctccagcc ccacgccgac gcggtccgag cacagggtgc acttgtaggc
     7921 cttgttgtcc tgcgggctga tgcgtggcac gtcgaacggg cagcccttga tgcagtagcc
     7981 gcagccgatg cagtgctcgc tgatgaagtc gacgatgccg ttggcgtact ttacgatcgc
     8041 gcccggtgac gggcaggcct tcaggcagcc cgggtcctcg cagtgcatgc agccgtcctt
     8101 gcggatcagc cattcgagct tgtcctgctc gggctcggcc tcgtagaagc gcatcaccgt
     8161 ccaggactgg gcggtgaggt tggtcgggtt gtcgtacacc ccgatgttgc tgcccacctc
     8221 gtcgcgcagg tcgttccact ccatgcacgc cacctggcag gccttgcagc cgatgcactt
     8281 ggagacgtcg atgagcttgg cgacctgcgg cgtgctgctg cgcgcctgag gcgggggggt
     8341 cgtcgtggcc gagcgggcga ccacgtcctg ggattgcagg ctggacatgg cttacacctt
     8401 ttcgatgttg acgaggaagg ccttgaactc cggcgtctgc gagttggcgt cgcccacgaa
     8461 cggagacagg gtgttggcca ggtagccgtt cttcgtctga cccttgaaac cccagtggaa
     8521 ggggatgccc accaggtgca ccttctggtc gttcacgttc cagggtttga tgcgcttggt
     8581 gaccaccgcc cgcgtcacga tgtggccgcg gttgctgctg accttgacct ggtcgccgtg
     8641 ccggatgttc ttttccttcg ccagctcttc gctgatttcg acgaacgatt cgggctgcac
     8701 gatggcgttg agccgcgcgt gcttggtcca ggtgtggaaa tgttcggtca gccggtaggt
     8761 ggtggccgag tacgggaact cctccttctt gccaaaggcc tccaggtcgc ccttgaacac
     8821 ccgcgccgcc ggattgctga tggccttggg gttgtcgggg tgcagggggt tgacgccgat
     8881 cggggtttcg aacggttcgt agtgctccgg gaacgggccg tcggccaggc cgatggcgtg
     8941 cagtcgggcc acgccctcgg ggttcatgat gaacgaaccg acgttttcct cgggtgcggc
     9001 gttgggccgc atgtcgggga cgtcggcgcc ggcccaggcc ttgccgttcc agtacacgta
     9061 cttcttgttc tccgaccacg gcttgccgtt cttgtcggcg ctggcgcggt tgtacaggat
     9121 gcggcggttg gccggccagg aaaagcccca gttctggtac acgcccagac cggtggggtc
     9181 ggatacgtcg cgccgcgcgg tcaggttgcc ggcttcgctc cagcagccgg cgtacagcca
     9241 gttgccgcag gcggtggaac catcgtcgcg cagcatggca aagcccggca cctgctgacc
     9301 cgccttcacc agcacctggc gcggcgccgt ggggttgttg gggtcgggcg gtgccagcac
     9361 gtcggacagc gccttgccgt tgatttcgcg cagcacttcg gtggccgatg gggcctcggg
     9421 ctgcacgtag tcccaggcca gaccggcgat ggcgtcgggg aaggcgccgc cgtccttggc
     9481 gtacatctga cgcagcttga ggaacagccg cgccatgatc tccgaatcca ccttggcttc
     9541 gccggggggt tcgagcgcgc gctccttcca gatcgcgatg cggctggagt tggtgaagct
     9601 gccttcctct tcggcaaagc aggccgaagg cagacggaac acctcggtct ggatgctttc
     9661 gggcttgacg tcgttgaagg ggccgtggtt ctgccagaac tcaccggttt cggtttccag
     9721 cggatcgatg atgaccaggt acttcagctt ggacagcgcc gccgacagct tgttcttgtt
     9781 gggcaccgtc gccagggggt tgaacccctg cgagatgaag ccggtcatct tgccctggtg
     9841 catcagctcg aacatgtgca gcgcatcgaa gccaccggcc agcttgggca cccagtcgta
     9901 gcagtactgg ttgtccgccg tggccgcctt gccgtagtag gacttcatca ggctgatgtg
     9961 aaagcgcggg aagttgctcc agtagttgaa ctggttcggc cgcatcggct tgggcgtgcg
    10021 cttggccagg tacttttcga aggtgtcgtc ggcgtcggtg ggcgcactca ggtagcccga
    10081 cagcacctcg gcgtaggcgt tcatgtcggt cacgccctgc acgttggagt gtccgcgcag
    10141 cgcgttcacg ccgccgccgg gccgccccat gttgcccagc agcagctgga tcatggccat
    10201 ggtgcggatg ttctggctgc ccacgctgtg ctgtgtccag ccgagcgcgt agaggatggt
    10261 gctggtcttg tcgggcgcgg acatctcgcc cagcatttcg cacaccttca ggaacttgtc
    10321 ctgcggcgtg ccgcagatgc ggctgaccat ctccggcgtg tagcggctgt agtgcttctt
    10381 gagctgctgg tagacgcaca gcgggtcctg caggctgtcg tcgaccttga cgtggccgtt
    10441 ctcgtcgagc tggtagcccc agctggcctt gtcgtacttg cgcttggcct cgtcgtagcc
    10501 gctgaagatg ccgttttcca gcttgaagtc gggcttgagc aggaaggtgg cgtcggtgtt
    10561 gaacttgacg tagtcgtgat ggatcttgtc gtggctcagc agccagttga tcacgccacc
    10621 caggaaggcg atgtccgtgc cgttgcggag cggcgcgtac aggtcggcca ccgaggaggt
    10681 gcggttgaag cgcgggtcga ccgaaatcag ccttgccttg cgcttttcca tggcatccac
    10741 cgcccatcgg aagccgaccg ggtgggcttc ggcggcattg ccgcccatga cgagaatgag
    10801 atccgtattg cggatgtcag tccacgaatt ggtcatcgct ccgcgaccga acgtcgggcc
    10861 cagactggtc accgtcggtg cgtgtcagac gcgtgcttgg gtatctatcc ccaccatccc
    10921 caaggcacgt gccaccttga cggacagata gctggattca ttggacgacg aactgcacac
    10981 cagaaaaccg gtggtgggcc agcggttgac cgtggtgccg gcgtcgttct tggcctggaa
    11041 gttggcgtcg cggtcggcct tcatgtgcct ggccacgcgg gtcagcgcct cgtcccagct
    11101 gatgcgcttc cattccttgc tgccagcctc gcgcaccgac gggtagatga cgcgcccggg
    11161 cgagttgatc atgtccatga cgccggcgcc cttgggacac agcgtgccgc ggttgaccgg
    11221 gttgtcggga tcgccttcca cgtggatcac gtgttccctg acgttcttgg cggtgtcgcc
    11281 cagcttgtag atcagcatgc cgcaactgac cgagcaatac gggcaggtgc tgcgcgacac
    11341 gctggcacgg gtgagtttga aggcgcgtgt ctcggccagg gccgcggtgg gtgaaaaacc
    11401 cagcgcgacc aggctggacg cggccaagcc agctccgctg acgcggaaga actggcgccg
    11461 gttcatgtcc atggggcggt ctcctctcgt gggatgcttt gcccgtgtcg acatgcacgc
    11521 cgaccgggat gcaccagccc tcagtctagg gagggtcagg gttggtgcca ctgaggtaaa
    11581 ccctatgtaa ggagtgttgc agcgccgccc ggcacacgcc gggcgtgcgc ggcctgccgc
    11641 cgcagagggt tcagtcgccc atcaccacgc cttcacgccg tggatcggcg ccgcccagcc
    11701 aggtcggttg gccatgcacg gtgccgcggg tgatggcctg caggccgctg gtcagattca
    11761 tctcgcgcac ttccgcgccg cgcgagcgca gggcttcgac ggtggcggcg gggaacttct
    11821 tttcctccag caggctgggg ccgttgagcg aaccgaagtt gggcaggttg atggcctgct
    11881 gcggcagcag gccccagccc agcacgccgt gcagggtctt ggcggtgtag tggatgatga
    11941 gggcgccgcc cgggctgccg gcgctcatca ccagcgggcc gcggcggccg tcggtggtct
    12001 tttcgaacac cagggtgggc gccatcgacg agcgcggccg cttgccgggc tgcacgcggt
    12061 tggcgatcgg ctggccctgg gcgtcggtcg gggcgaagct gaagtcggtc agttcgttgt
    12121 tcagcagaaa gcccttgacc atctgacggg cgccaaactg gtcctcgatg gtggtggtca
    12181 tggccagcgc gttgccgaag gggtcgacga tgctgatgtg gctggtgccg tattcgggct
    12241 ggctggccat gggggcgaag ctggttcggg tgccgacggg cttgcccggc ggcgccacct
    12301 tcaggctctg cgtgccgatc agcgcagcgc ggctcttcag gtaggccgga tccagcaggc
    12361 tcatccagct gccggcgggc ggctgcacga agtcggggtc ggccacgtac agcgcacggt
    12421 cggcaaacgc cagccgcgac gcttccatgt agtagtgcag ccaatcggcc gtgggcaacc
    12481 cgtcggcgcc ggccgccatg ctggccgccg gggtgtgggc caggatgccc aggatctggc
    12541 cgaccgcgat ggcgcccgac gacggcggcg gaaagccgca cacctcatag gccttgccgc
    12601 tggccggcac gcgcagttcg tggcacagcg gcgcgcgccg cttgggcgtg tagccggcca
    12661 ggtcggccag cgtcagtttg ccggggttgg tggggtgctt ctgcaccttg tcgacgatgg
    12721 cctgcgccac cgggccttcc agcagggcgg tcgagccgcg cgcggcaatg cccttgagca
    12781 ccgccgccag ttcggggttc ttcagcacat gcccgaccgg ccaggggttg ccggcggcgt
    12841 cgtagaagtg gcgggcggcc accgggtcct ttttcaggtg ctgctcgttc ttcagcaggg
    12901 tgtgcaggcg cgggctgacg gcaaaaccct gctcggccag cacgatggcg ggcttgaaca
    12961 gctcggccca ggccagacgg ccatgctgac ggtgtgcctg ctcgagcatg cgcaccgtgc
    13021 ccggtacgcc gaccgagcgg ccgccgacca cgccgtcatg gaacggcagg ggcttgccat
    13081 cggcgcccag gaacagcttg tcgtcgacgg cggccggcgc cgtctcgcgt ccgtcccagg
    13141 ccgtgacgtc acccccggcc gcgtgcagca ggaaggcgcc gccgccgatg ccgctggact
    13201 gcggctcgac cagggtcagc accatctgca ccgcgatggc ggcatcgagc gcgctgccgc
    13261 cggccttgag cacctggtag ccggcgtcgg tggccagcgg gttggcggcg gcgacggcga
    13321 acttgtgcgt cgcccagccc ggcttgtcgg tccagcccga gccgccttcg ggggtgaccg
    13381 gtgtcgccgg cggcgtgtag ttcaagctgc tggcgcagcc ggccagcagg gtggcggcgg
    13441 ccagggcggc cagggtggtg gggcggtggg gcaaggccat gcgagtctcc tggtgggtgg
    13501 ggtgagacgg catggtaacg cacggccggt gctgcgccgg cctggcgtga ttcctggtca
    13561 aacaggcatg tggtgcttgt ctggcaagcg ccgaaagcta tcaaaacaat agcgttcggg
    13621 atgcccggcg gtggccaccg cgcagccggc cgggcggagt ggcagggcct tacttgggtg
    13681 ccagccggat ggcaccgtcc agccggatca cctcgccgtt cagcatgtcg ttctcgatga
    13741 tgtggcaggc cagcttggcg tagtcctcgg gggtgcccag gcggctgggg aagggcacgc
    13801 cggcggccag cgcgtcctgc acctccttgg gcatgccaaa cagcatcggc gtgcccatga
    13861 tgcccggcgc gatggtcatg ttgcggatgc ccgtgcgggc cagatcgcgc gcgatcggca
    13921 gcgtcatgcc gacgatgccg cccttgctgg ccgaatacgc cgcctggccg atctggccgt
    13981 cgtaggcggc cacgctggcg gtgctgatca gcacgccgcg ctcgccggtg ggctcgggct
    14041 cgttgcggct catggcgtcg gccgccagac ggatcatgtt gaaggtgccc accaggttga
    14101 ccatgatggt gcgggtgtag ccgtccagcc ggtgcgggcc gttcttgcct acggtgcgct
    14161 cggcggtggc gatgccggcg cagttgacca ggcccatcag cttgcccatc gacaccgcct
    14221 tggccaccac ggcctggccg tcggcttcgc tgctgacgtc gcacttgacg aaggcgccgc
    14281 cgatctcgcg cgccaccgcc tcgcccttgt ccacctgcat gtcggcgatg accaccttgc
    14341 caccgcgcgc cgccagcatg cgcgccgtgc cttcgcccag accggacgcg ccgccggtga
    14401 cgatgaatac cttgtccttg atctgcatgg gagttgctcc agatgaggga aacgccaatg
    14461 gtacccagca atcccgcgcg catcgttgca aataagaatt attcgcatat actgacctcg
    14521 ccctcccgct ccaccccttc accgaggcca ccctatgtgc attcactgct ccccccacga
    14581 ccgccgcgac gacgacgagc tgcggcccac cgccgaggcc ttgctggccg ccaccctggc
    14641 gctgatgacg ggcgtggccg aggctgcacc cggcgccgcc ggcgtccggc cgatgagcct
    14701 gaaggtgctg gccaacctgg atgaactggc gcgccatccc ggcctgagtc ggtccatgcg
    14761 ccagctggcc gcgcacctcg gcctgcattg gcagcagctg gtgcagaccg cgcgtggcga
    14821 agcccgcgcc gccggcgagc gggcgcgctg ggtgccggca tcgacggtgg tgcaatgagt
    14881 gcgctggagc caggcgcgct gcagcgcgag caccagctgc tgctgcgcca tctgggccgg
    14941 gtgcagcagc gcgtgagcga gcagatgctt gcgcaggcgc agcgctgtgc ggcgctggag
    15001 gccgagctga tgcaatggcg ggcacgctgg atcgtcgcca ccacgcgccg cctgtggggg
    15061 ctgggcggct tcgatcagcc gccggcgcca gcccgtgccg tgccggccga ccgctcggcc
    15121 tgggccagcg ccaccgaggt gctgtgccag accggttgcg ccggccatgc ccatccgtgg
    15181 cgcgacgagc aggggcgatg cgcgctcacc gccagcgact gcacgcgtgt gccgccggtc
    15241 gcgcaggacg cttcagccgg cgccgtcgcc cgcgtgttct cgcgcccctg aggccatccc
    15301 cagcagccgc tgcaggcgcg cgctgctggt ggtgtattcg aacggcaggg ggtcgtgcgg
    15361 gtggatgtgc tcccgcgccg cgtaggcggc cagcgtggct tcgtgaaagc cgcacaccat
    15421 gagtttcttc ttgccggggt aggtgttgat gtcaccgatg gcgaagatgc ccggctcgct
    15481 ggtctggaag gtggccgcat ccaccaccag ctgcttgcgc tccagcgcca gttgccaggc
    15541 ggtgatcgga ccgagccgcg gactcacgcc ctgctgcacc agcagcagct cgagcggcag
    15601 cggctgcgtg ctgccgtccg gccccatcac ctgcaacgcg ctcaggcgcc ccgcggcctg
    15661 ctcgaagccc accacctggc ccacccgaag gcgcatcgag ccggccgcgc acaacgcgcg
    15721 cacccgcgcc tgggtggccg gcgcggcctg aaaaccgtcg cgtcggtgca gcagggtgac
    15781 gctggcgggc gcggcggccg ccagcgtggc cagccggatg gcctgcgtca gcgccggctc
    15841 gccgccgccg acgacgacca cgtgctggcc ggccagcgcg gcgtctgccg gtgcctggtg
    15901 gaacagctgg cgccgctcga acgcttccag tccggcgacc ttcaggcgtc gcggcaggaa
    15961 ggcgcccaca ccggcggcga tgaacacgct gcgcgcccgc aggcgccggc caggctcggt
    16021 ctgaagcaga aagcgcccgt cgggcaggcg ctgcagcgac tccaccaggt gaccgaggtg
    16081 ccactggggc gcaaagggcg cgatctgacg cagcagcgcg tcggtcagcg tctgcccgct
    16141 gcacaccggc accgccgcga tgtcgtagat cggcttgtcg gcgtacaact cgaccggctg
    16201 accgccggca tggggcaggg catcgatcac atgggcgcgc agctccagca ggcccagctg
    16261 gaacaccgcg aacagcccca ccggcccggc gccgatgatc gcgacgtcgg tatcgagcgt
    16321 gggggcaggg cggtgcgggt tcatttggtg cagtgtttcg cgctgtcagg tacttatagc
    16381 gagtcgccaa ggggccagct gc
//
LOCUS       DAOOOG010000037        16372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_3742_length_16372_cov_20.182080, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000037 DAOOOG010000000
VERSION     DAOOOG010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 16372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16372
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3742_length_16372_cov_20.182080"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PK242_04725"
     CDS             complement(<1..295)
                     /locus_tag="PK242_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOZ93352.1"
                     /translation="MSTSWIERLFKLEEHGTNVRTELIAGLTTFLTMAYIIFVNPAIL
                     GDAGMPKDAVFVATCLIAALGTAVMAFYANYPIALAPGMGLNAYFAYAVVLHMG"
     gene            complement(312..1325)
                     /locus_tag="PK242_04730"
     CDS             complement(312..1325)
                     /locus_tag="PK242_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ93353.1"
                     /translation="MNTKDVAPNPAPAGSTGDRGPLERIDLHLIRVLHTVLTEGSVSR
                     AALRLGMHQPAVSAALKRLRDLAGDPLLVRHGAQMVATDAGRRMIEPAASILRTAETL
                     FTEAQRFDPRTSTQRFHIAASDYLDPLFLPHLVADIQRQAPLTRVEIHALTGEADYRA
                     QLAQGEIDLVIGNWLQPPDELHMAHLFSDEVVSLVSHRHPAARRGWSEADWLEAEHIA
                     PMPNYPGARGIIDQVLDERGLQRRIAVRCAYFGLIPQMVADSLLVLTTGRQYCERYAR
                     QLPLTILPCPVAMPPLLYYMLWHTRSQHANAARWLRERTRQVISELRTGGPRHPSTPS
                     HKP"
     gene            complement(1376..3802)
                     /gene="xdhB"
                     /locus_tag="PK242_04735"
     CDS             complement(1376..3802)
                     /gene="xdhB"
                     /locus_tag="PK242_04735"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HOZ93354.1"
                     /translation="MNHPVADPLLHAALPFADYRHNQQARLDSAAEAQAEQAGARVGI
                     SRPHESATLHVTGGATYIDDLPELAGTLHCALGLSPVAAGRLQGFDRDALAAMPGVVR
                     VLTAADIPGVNDCGSIVHDEPILCDGEIRFLGQPVFAVVAETREQARRAAALARQVLR
                     VDAAEPVLTPRQAHEKGQYVVPPMHLVRSASGLDEAGIRAAIARAPHRLSGSLDVGGQ
                     EQFYLEGQISYAIPKEGRGMHVHCSTQHPSEMQHLVAHALGVPAHAVLVECRRMGGGF
                     GGKESQSAQFACIAAVAAQALQRPVKLRLDRDDDFMVTGRRHGFWYEYEVGHDDEGRI
                     LGACLQMVSHAGHSADLSAPVMTRAICHFDNAYWLPEVAMHGYCGKTHTQSNTAFRGF
                     GGPQGAIAIEYILDSIARRLGRDPLAVRRANFYGQSERNVTPYGQTVRDNVIHELVDQ
                     LEASSDYDGRRAAIAAFNAGSPVLKRGIALTPLKFGISFNVRHFNQAGALVHVYTDGS
                     ILVNHGGTEMGQGLNTKVAQVVAHELGVGFERVRVTATDTSKVANTSATAASTGSDLN
                     GKAAQDAARQIRERLAACAAGRHGGLAAEVRFANDQVQVGGRTLDFATVVGEAYLDRV
                     QLWSDGFYATPGLSWDREKMQGHPFYYFAYGAAVSEVLVDTLTGEWKLLRADVLHDAG
                     RSLNPALDIGQVEGAFIQGMGWLTMEELVWHPKTGLLSTHAPSTYKIPTANDCPPVFN
                     VRLFEGRNAEDAIHRSKAVGEPPLLLPFSVFFAIRDAVSAAGGHRIDPPLQAPATSEA
                     ILRALQAVRA"
     gene            complement(3824..5371)
                     /gene="xdhA"
                     /locus_tag="PK242_04740"
     CDS             complement(3824..5371)
                     /gene="xdhA"
                     /locus_tag="PK242_04740"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HOZ93355.1"
                     /translation="MTTLSPPTASPRAIRFYHRGTLVSVDGVPVTRTVLDWLREQRRL
                     TGTKEGCNEGDCGACTVVLGELDASGQLRLSSVNACIQFLPTLDGKALFTVEDLQALG
                     GAAPDALHPVQQAMVDCHGSQCGFCTPGIVMSLWNTYERHQQAGTQPTRQQLADDLSG
                     NLCRCTGYRPILDAGQRMFDLPPVALDRQAVTEALRALQREPALHYAAPQPTPAGPRT
                     DHFHAPRTLQQLAELRLAKPQARLLAGSTDIGLWVNKQFRDVGDLIYVGHVAELRAIE
                     ARGDALWIGAAAALEDAWRALVRRAPTLTDVWLRFASLPIRHAGTLGGNVANGSPIGD
                     APPILMALDAAVELRRGERVRRLPLTELYLDYMKNAIEDGEFVQAIVVPDAAFRRQLR
                     GYKVSKRFDCDISALCAGMAIELDAGVVRSVRLAYGGMAATVRRAAEAEAAITGQPWS
                     EATVRTAQQALARDFTPLTDMRASSAYRLQVAQNLLQRLWLETRPENPLPPAATSVFH
                     LMPHAAA"
     gene            complement(5559..6905)
                     /gene="argA"
                     /locus_tag="PK242_04745"
     CDS             complement(5559..6905)
                     /gene="argA"
                     /locus_tag="PK242_04745"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HOZ93356.1"
                     /translation="MSTVFNFTFVPWFRSVAPYIHMHRGKTFVVGVVGEAIAAGKLAA
                     IATDLALIQSMGVKIVLVHGFRPQVNEQLQAKGHEPRYAQGMRITDEVALDCAQEAAG
                     QLRYEIEAAFSQGLPNTPMAGSRVRVISGNFLTARPMGIIDGVDFQSTGVVRKVDAAG
                     IRHSLDSGALVLISPFGFSPTGEAFNLRMEEVATSVAIALQADKLLFLTEVPGIRVHP
                     ESPAAEDNPIDTEIPLADAQALLARLPAPEQPTDTAFYLRHCVSACLHGVERSHILPY
                     ATDGALLLEIYVHDGIGSMVVDEKLESLREAGADDVTGILHLIEPFEKDGTLVKRSRT
                     DIERDIANYTILEHDGVIFACAALYPYPEAATGEMAALTVSPQSQGQGDGEKVLRRIE
                     QRAREMGLKSIFVLTTRTMHWFIKRGFQQVDPDWLPEARKRKYNWDRRSQVLVKHL"
     gene            complement(7102..7605)
                     /locus_tag="PK242_04750"
     CDS             complement(7102..7605)
                     /locus_tag="PK242_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HOZ93357.1"
                     /translation="MEHFLERVMFASRWLLAPFYIGLVGAIAILLVKFVKEFVALLPQ
                     VLTLEGGQVMVGVLTLVDVALVANLLLIIVFAGYENFVSKINTGDSEDRPEWMGHVTF
                     SDLKIKLIGSIVAISGIELLKAFVNIDSHSNQQIAWKIALHMTFVLSGVLFAVMDRLT
                     AGKSGGH"
     gene            complement(7914..9041)
                     /locus_tag="PK242_04755"
     CDS             complement(7914..9041)
                     /locus_tag="PK242_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93358.1"
                     /translation="MTPSPAPTRACRLRTLQRGIAILSLGALIAGCGGGSGDGATPPA
                     PAPTPAPAPAPAPSPAPAPAPSPGNATYGDLRAAALGVGASLNGALPFPADNAWNTDI
                     SARPVDPNSDALIASIGLDRGLHPDFGAGLYQGQPIGIPYVVVPGTQPRVAVQFTDYG
                     DESDPGPYPIPPDAPIEGGPASSGDRHVIVIDRDAGRLYEMGNSYPQADGSWRASGGA
                     VFHLDSNAVRPTAQPGWTSADAAGLPIFPGLVRYDEAALGPGGIRHALRFTVSRTRRA
                     YVPPATHWASSNTSADLPPMGMRVRLKASYVIPAGFSAETRAILTALKTYGMMVADNG
                     SNWYLSGAPDERWNNDRLVSELGQVAGRHFEVVRMEGVVTP"
     gene            9321..9656
                     /locus_tag="PK242_04760"
     CDS             9321..9656
                     /locus_tag="PK242_04760"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HOZ93359.1"
                     /translation="MTYQNDRNQCLATPSIRRLEKLIQSGAADSHHVAFTKHALARMR
                     QRGITRIVVLDALRLGCIRQQPEPDIRHPGVKCRMERLVSGVLVGAVVYLEYPAPDLT
                     VVTVIDLGV"
     gene            9659..10177
                     /locus_tag="PK242_04765"
     CDS             9659..10177
                     /locus_tag="PK242_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93360.1"
                     /translation="MYHYTDGGLRNVWLGNGYTIRDTPYGQAVAIQDLEGLTAAICDA
                     FVRQPKPLTQTEFRYLRTSGLQLSQPALGAALGVDAQTIARWEKGAYIPKMADKLVRL
                     FYLNHANGNVRLSSAFESLRAVERAAHGPQPMRVIAEAKGRHWGARLESDGDPNPQAA
                     PIRRRRQPSTTH"
     gene            10699..11001
                     /locus_tag="PK242_04770"
     CDS             10699..11001
                     /locus_tag="PK242_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HOZ93361.1"
                     /translation="MKATLLLKSREVLSTTAFVERVLWLLPEPLSGSTHRLKYRLAFV
                     VDGVCVVRYDNERGKGDHRHVGDLESDYPFSTPEVLLNDFWRDVAQWRQTHEHRDD"
     gene            10982..11338
                     /locus_tag="PK242_04775"
     CDS             10982..11338
                     /locus_tag="PK242_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOZ93362.1"
                     /translation="MNTVTIELTDRQTQDARFLQAFKTGRPQGASIAFDSVEVLWQVL
                     TPKRWQIVRTLCGAGPMSLREAARRVGRDVKAVHGDVHALLTAGVLERDAAGRIVFPY
                     DAVHVDFTVQPEKAVA"
     gene            complement(11452..16191)
                     /locus_tag="PK242_04780"
     CDS             complement(11452..16191)
                     /locus_tag="PK242_04780"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOZ93363.1"
                     /translation="MLHAALQQFNNNRLRSYEADSGLLREHAGIEETVLAGGYGYRQI
                     LELVQNGADALLEAKESGGPQSSDGRIHVQLHGSRLYVANTGAPLSEEGLDALLRSHS
                     SPKRRNQIGRFGLGFKSLLKLNGQIDVFTRSSGAIRFDPERCRDELRRQFNVKQAPGL
                     RLVWPLPPDERAKDPICAELAWAETIVRAEVDSAELLLHLQNEIRGFPAEFLLFLPIA
                     VTLTLDFGEENIRTVRVVKHRGETILQDGEATSLWNIITRDVRITESRAVADATHIHA
                     RECVPLSWAMPLEGRREESGRFWAFFPTHTQTYLPGILNAPWKLNSDRNAIIGGEWNA
                     ALMTEAASLIAEMMPNLATSDDPARPLDAFPRQLERKDEIAVPLVQSLWARLETAVVI
                     SDGTGALRSANELWRHPNDDPALAVEWQSLAGPEQQRQLIHASCLQRQRASRLNALAE
                     RIEAQTTEQTSPELRRCRPAAWFRAVASTQTDVAIGVLHLAKAFSRVNADEWGEIRPT
                     LTIIPTQSGKLATASQVVFTPVGVPVPGRDLITAELQFNPETREILRSALLVRDLDDA
                     VWISILGEALPSSNWSSITEEEGWETFWECLRNAPAGARNRFLGTHSESVRVRRRDGS
                     WVKADRVVFPGELVSDGESVPNVNILVDPDTHAKDRASLESLGVLRVPAGDVGPIRFP
                     EFTGETWLNPWLEECRSRYKETHQNSASWHYLKPYPFSMPRGFRLLTELTGVPKTRYT
                     NQLLNRLAEPAYQKSIPFGHSTMSVYPKIDVPHPLLWYVHDHGALQIGTLSAPLKAVF
                     LRRDQPILKHLPEWECTAAKLHLLNVDFPEVTVTQSDLRTFWVAMFKAIITPHSLESD
                     VLTDLWLGAAKDGVVPIELPSLTGHVPLSAAFVTTSADLAQRARTPERVVVVLDQPTM
                     QLWQKAGAQDLASLIRTEWSDIAGPLERLIDVMPELGDVLRADIRDAARCQRVTKLCL
                     GIAGSAQAAPCLMRDGVLFLDAEQLAPLSRAERLKRLFTEIAPSGWLDDSPEDALARL
                     GDTGVDKCRTYVREGATLAERLLRAAGNRVEPLLDALGQRLRDVDFVRQCTPNQLADL
                     VLAQLGPAALSTLKDTLRDEGLQPPARWNSADALGFVAAIGFPPEFASASQARREPEE
                     LVSGPIELPPLHAFQQEVFDGLRSLLANGTQRRRAVVSLPTGGGKTRVTVQAAVQLVL
                     APENTTRSVLWIAQTDELCEQAVQAFRQVWVNLGAQKTSLRIARLWGGNRNPTPSDSD
                     KPVVVVASIQTLNSRISQAELAWLRKLGLVVIDECHHAITPSYSELLRWLDAEAPRPG
                     ATPKDEPPIVGLSATPFRTDDEESQRLARRFDNRWLPSNQEQLYARLRAQGVLAQAQY
                     ESLETGADLTDEELHKLGQLREPWEGLDFENLLESINQRLADDAQRNERLVECIRASA
                     EHSILFFTNSVTHAEEMAARLNLSGIGAAAISGDTPNAARRYFLDRFQNGKIRVLCNH
                     SVLTTGFDAPRTDMVLIARQVFSPVRYMQMVGRGLRGEKNGGTASCRIVTVLDNLGRF
                     ADRHPFHFCKGLYSPAGQSRQSQSLSN"
     gene            complement(16236..>16372)
                     /locus_tag="PK242_04785"
     CDS             complement(16236..>16372)
                     /locus_tag="PK242_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ93364.1"
                     /translation="LKALETELIHQAVRDAGGRVAVAAKALGVSRATMYRRLAGGSKV
                     "
BASE COUNT         2731 a         5232 c         5521 g         2888 t
ORIGIN      
        1 agcccatgtg cagcaccacg gcataggcga agtaggcgtt cagccccatg ccgggcgcca
       61 gcgcgatcgg gtagttggcg tagaaggcca tcaccgccgt gcccagcgcg gcgatcagac
      121 aggtggcgac gaacaccgcg tccttgggca tgccggcgtc gcccaggatc gccgggttga
      181 cgaagatgat gtaggccatc gtcaggaagg tggtcaaccc ggcgatcagc tcggtgcgta
      241 cgttggtgcc gtgttcttcc agcttgaaca ggcgctcgat ccaactcgtg ctcattgtgg
      301 tggtctcctc gtcatggctt gtgggaaggg gtggacgggt ggcgtggccc cccggtgcgc
      361 agttcgctga tcacctgtcg tgtgcgttcg cgcagccagc gcgcagcgtt ggcgtgctga
      421 ctgcgggtgt gccacagcat gtagtacagc agcggcggca tggcgaccgg acagggcagg
      481 atggtgagcg gcagctgcct ggcgtagcgc tcgcagtact gccggcccgt ggtcagcacc
      541 agcaggctgt cggccaccat ttgcggtatc aggccgaaat aggcgcagcg cacggcgatg
      601 cgccgctgca ggccgcgctc gtcgagcacc tggtcgatga tgccgcgtgc tcccgggtag
      661 ttgggcatgg gggcgatgtg ctcggcctcc agccagtcgg cttcgctcca gccgcggcgc
      721 gcggccgggt ggcgatggct gaccagcgac accacttcgt cgctgaacag atgcgccatg
      781 tgcaactcgt cgggcggttg cagccagttg ccgatcacca ggtcgatctc gccctgcgcc
      841 agctgcgcgc ggtagtcggc ctcgcccgtc agcgcatgga tctccacccg cgtcagtggc
      901 gcctggcgct ggatgtcggc caccagatgg ggcagaaaca gcgggtccag gtagtcgctg
      961 gcggcgatgt gaaagcgctg cgtgctggtg cgcggatcaa agcgctgggc ctcggtgaac
     1021 agcgtctcgg ccgtgcgcag gatgctggcg gcgggctcga tcatgcggcg tccggcatcg
     1081 gtggccacca tctgcgcgcc atggcgcacc agcagcgggt cgcccgccag atcgcgcagg
     1141 cgcttgagcg ccgccgacac cgccggctgg tgcatgccca ggcgcagcgc ggcgcgcgag
     1201 acgctgcctt cggtcagcac ggtgtgcagc acgcggatca ggtgcaggtc gatgcgctcc
     1261 agcggcccgc gatcgcccgt cgagccggcg ggcgcagggt tgggcgcgac atccttggtg
     1321 ttcatgaacg ggcaactccg gattcgatgc ggcggcaaga catggcgcca gcggctcagg
     1381 cgcgcaccgc ctgcagcgcg cgcaggatgg cttcgctggt ggcgggcgcc tgcagcggcg
     1441 ggtcgatgcg gtgaccgccc gccgccgaaa cggcgtcgcg aatcgcaaag aacaccgaaa
     1501 acggcagcag cagcggcggc tcgcccacgg ccttgctgcg gtggatggcg tcttcggcgt
     1561 tgcggccctc gaacaggcgc acgttgaaca ccggcgggca gtcgttggcc gtgggaattt
     1621 tgtaggtgct gggcgcgtgg gtgctcaaga ggccggtctt ggggtgccag accagctctt
     1681 ccatggtcag ccagcccatg ccctggatga aggcaccttc gacctgcccg atgtccagcg
     1741 ccgggttgag cgagcggccg gcgtcgtgca gcacgtcggc gcgcagcagc ttccactcgc
     1801 cggtcagggt gtcgaccagc acctcgctca ccgccgcgcc gtaggcaaag tagtagaacg
     1861 ggtggccctg catcttctcg cggtcccacg acagaccggg cgtggcgtag aagccatccg
     1921 accagagctg cacgcggtcc aggtaggcct cgccgaccac ggtggcaaag tccagcgtcc
     1981 ggccacccac ctgcacctgg tcgttggcaa agcgcacctc ggcggccaac ccgccgtggc
     2041 gcccggcggc gcaggcggcc agccgttcgc ggatctggcg cgccgcgtcc tgcgccgcct
     2101 tgccattcag gtcggagccg gtggacgccg ccgtggccga ggtgttggcc accttgctgg
     2161 tgtcggtggc ggtcacgcgc acgcgctcga agcccacgcc cagctcatgc gccaccacct
     2221 gcgccacctt ggtgttcagg ccctggccca tttcggtgcc gccgtggttg accaggatcg
     2281 agccgtcggt gtagacatgc accagcgcgc cggcctggtt gaagtgcctg acgttgaagc
     2341 tgatgccgaa cttcagcggc gtcagcgcga tgccgcgctt gagcaccggg ctgccggcgt
     2401 tgaaggccgc gatggcggcg cgccggccgt cgtagtcgct gctggcctcc agctgatcga
     2461 ccagctcgtg gatcacgttg tcgcgcacgg tctggccgta gggcgtgacg ttgcgttcgc
     2521 tctgcccgta gaagttggcc cggcgcaccg ccagcgggtc gcggcccagc cggcgtgcga
     2581 tgctgtcgag gatgtattcg atggcgatcg ccccctgtgg gccgccaaag ccgcgaaacg
     2641 ccgtgttgct ctgcgtgtgg gttttgccac agtagccgtg catggccact tcgggcagcc
     2701 agtaggcgtt gtcgaagtgg cagatggcgc gcgtcatcac cggcgccgac aggtcggccg
     2761 aatggccggc gtgcgacacc atctgcaggc aggcgcccag gatgcggccc tcatcgtcgt
     2821 ggccgacctc gtactcgtac cagaagccgt ggcgccggcc ggtgaccatg aagtcgtcgt
     2881 cgcggtccag ccgcagcttg accgggcgct gcaacgcctg cgccgccacc gccgcgatgc
     2941 aggcaaactg cgccgactgc gattccttgc cgccaaagcc gccgcccatg cgccggcatt
     3001 ccaccagcac ggcgtgcgcg ggcacgccca gcgcatgcgc caccaggtgc tgcatctcgc
     3061 tcgggtgctg ggtcgagcag tgcacgtgca tgccgcgccc ttccttgggg atggcgtagc
     3121 tgatctggcc ttccaggtag aactgctcct gcccgcccac gtccaggctg ccgctcaggc
     3181 ggtgcggggc gcgggcgatg gcggcccgga tgccggcctc gtccagcccg ctggcgctgc
     3241 gcaccaggtg catgggtggc accacgtact gacccttttc gtgcgcctgg cgcggggtca
     3301 gcaccggctc ggcggcgtcc acccgcagca cctgcctggc cagcgccgcg gcgcggcgtg
     3361 cctgctcgcg cgtttcggcc accacggcaa acaccggctg gcccaggaag cggatctcgc
     3421 cgtcgcacag gatcggctcg tcgtgcacga tcgagccgca gtcgttgacg cccgggatgt
     3481 cggccgccgt gagcacgcgc accacgcccg gcatggcggc cagcgcgtcg cgatcgaagc
     3541 cctgcaggcg gccggcggcc acgggcgaca ggccgagcgc gcagtgcagg gtgccggcca
     3601 gctcgggcag gtcgtcaatg taggtcgcgc cgccggtcac gtgcagggtg gccgattcgt
     3661 gcggacggct gatgcccacg cgcgcgccgg cttgctcggc ctgggcttcg gcggcgctgt
     3721 ccaggcgggc ctgctggttg tggcggtagt cggcaaaggg cagggcggcg tgcagcaggg
     3781 ggtcggcaac agggtggttc atggtcgggc tcccggtagc cgctcaggcc gcggcgtgcg
     3841 gcatgaggtg gaacacgctg gtggcggccg gtggcagcgg gttttcgggc cgggtttcca
     3901 gccacaggcg ctgcagcagg ttctgcgcca cctgcaggcg ataggcgctg ctggcgcgca
     3961 tgtcggtcag cggcgtgaag tcgcgcgcca gcgcctgctg ggcggtgcgc acggtggctt
     4021 cgctccaggg ctgcccggtg atggccgcct cggcctcggc ggcgcgccgg acggtggcgg
     4081 ccatgccgcc gtaggccagg cgcacgctgc gcaccacgcc ggcgtccagt tcgatcgcca
     4141 tgccggcgca cagcgccgag atgtcgcagt cgaagcgctt gctgaccttg tagccgcgca
     4201 gctggcgccg gaaggcggcg tcgggcacga cgatggcctg cacgaactcg ccgtcttcga
     4261 tggcgttctt catgtagtcc aggtaaagct cggtgagcgg caggcggcgc acgcgttcgc
     4321 cccggcgcag ctcgaccgcg gcgtcgagcg ccatcaggat cggcggcgca tcgccgatcg
     4381 gcgagccgtt ggccacgttg ccgcccaggg tgccagcgtg gcggatcggc agcgaggcaa
     4441 agcgcagcca gacgtcggtc agcgtcgggg cgcggcgcac cagggcgcgc caggcgtctt
     4501 ccagtgcggc ggcggcgccg atccacagtg cgtcgccgcg cgcttcgatc gcgcgcagct
     4561 cggccacgtg gccgacgtag atcaggtcgc ccacgtcgcg aaactgcttg ttgacccaca
     4621 ggccgatgtc ggtcgagccg gccagcaggc gggcctgcgg tttggccagg cgcagctcgg
     4681 ccagctgctg caacgtgcgc ggggcgtgga agtggtcggt gcgcgggccc gcgggcgtgg
     4741 gctgcggcgc cgcgtaatgg agcgccggct cgcgctgcag ggcacgcagc gcctcggtca
     4801 cggcctgacg gtcaagcgcc accggcggca ggtcgaacat gcgttggccg gcgtcgagga
     4861 tgggacggta gccggtgcag cggcacaggt tgcccgacag gtcgtcggcc agctgctggc
     4921 gcgtgggctg ggtgccggcc tgctggtggc gttcataggt gttccacagc gacatcacga
     4981 tgcccggcgt gcagaagccg cattgcgagc cgtggcaatc caccatcgcc tgctgcaccg
     5041 ggtgcagggc gtcgggcgcg gcgccgccca gcgcctgcag gtcttcgacc gtgaacaggg
     5101 ccttgccgtc cagcgtgggc aggaactgga tgcaggcgtt cacgctgctc aggcgcagct
     5161 ggccgctggc gtcgagttcg cccagcacca cggtgcaggc gccgcagtcg ccttcattgc
     5221 agccttcctt ggtgccggtc aggcgccgct gctcgcgcag ccagtcgagc acggttcggg
     5281 tgaccggcac gccgtccacc gacaccagcg tgccgcggtg atagaagcgg atggcccggg
     5341 ggcttgcggt cgggggactg agggtcgtca tctgcggctc cggcggtcaa ggtctgcccc
     5401 ggggatgggt cagccaaccg ttgcgcccgc gatggtgcgc gggctggccg ccccgtggca
     5461 tacagggggc acgatagcgg atgtatgggt cgcgttatga ctcgggggtt aaccccgaga
     5521 ggggcgcagg gtcggcgggc gctgggtgct gccggcgttc acaggtgctt gaccagcacc
     5581 tggctgcggc ggtcccagtt gtacttgcgc ttgcgcgcct cgggcagcca gtcggggtcg
     5641 acctgctgga agccgcgctt gatgaaccag tgcatggtgc gcgtggtcag cacgaagatg
     5701 ctcttgagcc ccatttcgcg tgcgcgctgc tcgatgcgcc tgagcacctt ttcgccgtcg
     5761 ccctggccct ggctttgtgg cgacaccgtg agcgcggcca tttcgccggt ggcggcttcg
     5821 gggtaggggt agagcgcggc gcaggcgaag atcacgccgt cgtgctccag gatggtgtag
     5881 ttggcgatgt cgcgctcgat gtcggtgcgg ctgcgcttga ccagggtgcc gtccttctcg
     5941 aacggctcga tcaggtgcag gatgccggtg acgtcgtcgg cgccggcttc gcgcaggctt
     6001 tccagcttct cgtcgaccac catgctgccg atgccgtcgt gcacatagat ttccagcagc
     6061 agcgcgccgt cggtcgcgta gggcaggatg tggctgcgct ccaccccgtg cagacaggcg
     6121 ctgacgcagt ggcgcaggta gaacgcggtg tcggtgggct gctcgggggc gggcagccgc
     6181 gccagcagcg cctgcgcgtc ggccagcggg atttcggtgt cgatcgggtt gtcttcggcg
     6241 gccgggcttt cggggtgcac gcggatgccg ggcacctcgg tcaggaacaa cagcttgtcg
     6301 gcctgcaggg cgatggccac cgacgtcgcc acttcttcca tgcgcaggtt gaaggcctcg
     6361 cccgtgggcg aaaagccgaa cggtgaaatc agcaccagcg cgcccgaatc cagcgagtgg
     6421 cgaatgccgg cggcgtccac cttgcgcacc accccggtgc tctgaaagtc gacgccgtcg
     6481 atgatgccca tgggccgcgc ggtgaggaag ttgcccgaga tcacgcgcac ccgcgagccg
     6541 gccatcggcg tgttgggcag gccctggctg aaggcggcct cgatctcgta gcgcagctgc
     6601 ccggcggcct cctgggcgca gtccagcgcc acctcgtcgg tgatgcgcat gccctgcgca
     6661 tagcgcggtt cgtgcccctt ggcctgcagc tgctcgttga cctgggggcg aaagccgtgc
     6721 accagcacga tcttcacgcc catgctctgg atcagcgcca gatcggtggc gatggccgcc
     6781 agcttgccgg cggcgatggc ttcgcccacc acccccacca cgaaggtctt gccgcggtgc
     6841 atgtggatgt acggcgccac cgagcggaac cagggcacga aggtgaagtt gaagacggtg
     6901 gacatgcgcg gcctgaaggg gtgcgtgaag cccggcccag gggccggctg ccgcacagtg
     6961 taacgatccg cgcgcaccgg cggcggccct cgcggtgctg cacaactatg aaaacagtag
     7021 ctggtggcgc ttgccagaca agcgccagag gccaatctga cggtgcaacg cgccagcccg
     7081 gccggtgcgc cccggtggcc gtcagtgacc gcccgacttg cccgccgtca ggcggtccat
     7141 cacggcaaac agcacgcccg agagcacaaa ggtcatgtgc agcgcgatct tccaggcgat
     7201 ctgctggttg ctgtggctgt cgatgttgac gaaggccttc agcagttcaa tgcccgaaat
     7261 cgccacgatc gagccgatga gcttgatctt caggtcggaa aaagtgacgt gccccatcca
     7321 ttccggccga tcctcgctgt cgccggtgtt gatcttggag acaaagtttt catagccggc
     7381 aaacacgatg atgagcagca ggttggccac cagggccacg tcgaccagcg tcagcacccc
     7441 gaccatgacc tgcccgccct ccagcgtcag cacctgcggc agcagggcga cgaactcctt
     7501 cacgaacttg accagcagga tggcgatggc gcccaccaga ccgatataga aaggcgccag
     7561 cagccagcgg ctggcgaaca tgacgcgttc cagaaaatgt tccattgatt tcatgatgca
     7621 gtgcatgcca taggttcaag ccccggcgcc ggggaagggc cacggcccac ctggcgcagt
     7681 cgccacggca agccatgcag tgccgccagg gcggctcgac acttctgaaa tgatagcttc
     7741 tggcgcttgc catgcccggg ctggcggcca gaacgacccg tgattcgcag accaacccgg
     7801 gcggtccgtg cccgcgtgcc cgcccgcagg gcccgggggc gccccgatgg ccgatggctg
     7861 cccacggcag gcccaggtcg gcgccacctt gcgaaggcgg gcgcaggcgg cccctacggc
     7921 gtcaccaccc cctccatccg caccacctcg aagtggcggc ccgccacctg gcccagctcg
     7981 ctcaccagac ggtcgttgtt ccagcgctcg tcgggcgcgc cgctcaggta ccagttggag
     8041 ccgttgtcgg ccaccatcat cccgtaggtc ttcagcgccg tgaggatggc gcgcgtttcg
     8101 gccgagaagc cggcggggat cacgtagctg gccttcagcc gcacgcgcat gcccatgggc
     8161 ggcaggtcgg cgctggtgtt gctggatgcc cagtgcgtgg ccggcggcac gtaggcgcgg
     8221 cgcgtgcggc tcaccgtaaa gcgcagggcg tgccggatgc cgcccgggcc cagcgcggct
     8281 tcgtcgtagc gcaccaggcc gggaaagatg ggcaggccgg ccgcgtcggc gctggtccag
     8341 cccggctggg cggtggggcg tacggcgttg ctgtccaggt ggaacaccgc gccgcccgag
     8401 gcgcgccagc tcccatctgc ctgcgggtag ctgttgccca tctcgtacag gcggcccgcg
     8461 tcgcggtcga tcacgatcac gtgccggtcg ccgctgctgg ccggcccgcc ctcgatcggc
     8521 gcatcgggcg gaatggggta ggggccgggg tcgctctcgt cgccatagtc ggtgaactgc
     8581 accgccacgc gcggctgcgt gcccggcacc accacgtagg ggatgccgat cggctggccc
     8641 tggtacagcc cggcgccaaa gtccgggtgc aggccgcggt ccagcccgat gctggcgatc
     8701 agcgcgtcgg agttcggatc gaccgggcgc gcgctgatgt cggtgttcca ggcgttgtcg
     8761 gccgggaagg gcagcgcccc gttcagcgac gcgcccacgc ccagcgcggc ggcgcgcagg
     8821 tcgccgtagg tggcgttgcc gggggagggt gcgggtgccg gggccgggga cggggcggga
     8881 gcgggagcgg gagctggcgt gggtgctgga gcgggcggcg tggcgccatc cccgctgcca
     8941 ccaccgcaac ctgcgatcag cgcgcccagg ctgaggatgg cgatgccacg ttgcagggtg
     9001 cgcaagcggc atgcgcgggt gggtgcagga gagggagtca tgggcgtcat cttgaaggat
     9061 ggcgcagtgt gcccgagccg gcgccttcgc caccgcgtgg atgcgtttcg tgcgcggtgg
     9121 aatggtgaga aagcgtgtag agtccgtaga ttcttcaacc caaatcggcc tccagagctt
     9181 taccatcaag cgcaagcagc tatcagttca atagtaattg gctttgccgc gatggtccct
     9241 cccattcccc gttccgccgt gggagtggcg gcacacagca ggggtaatcc caacccaatc
     9301 agccagccgg atgcggtaac atgacctatc aaaatgatag gaaccaatgt ttggccaccc
     9361 catctatcag gcggctcgaa aagctgatcc aaagcggcgc cgcagacagc catcatgttg
     9421 cctttaccaa gcacgcgctg gcgcgcatga ggcagcgcgg catcacgcga attgtggtgc
     9481 tggatgcgct gcggctgggg tgcatacgcc agcagccgga gccagacatc aggcaccccg
     9541 gcgtcaagtg ccgcatggaa cggctggttt ccggcgtgct ggtgggagcc gtggtgtatc
     9601 tggagtatcc cgcccctgat ttgacagtag tcacggtgat tgatttagga gtctgagcat
     9661 gtaccactac accgacggcg gattgcgcaa cgtgtggctt ggcaacggct acaccatccg
     9721 cgacacaccc tacgggcaag ccgttgccat ccaggacctg gagggcctga ctgcggccat
     9781 ctgcgatgcc ttcgtgcggc aacccaagcc cctcacgcaa accgagtttc gctacctgcg
     9841 cacctccgga ctgcaactgt cacagccagc gctgggcgca gcgctggggg ttgacgcgca
     9901 gaccattgct cgctgggaga aaggggcata tatccccaaa atggccgaca agctcgtgcg
     9961 gctgttctac ctcaatcacg ccaacggcaa cgtccgcctg agcagcgcat tcgagtccct
    10021 gcgcgccgtc gagcgcgccg cgcacgggcc gcagcccatg cgggtcattg ccgaggccaa
    10081 gggtcggcac tggggagccc gcctggaaag cgacggtgat cccaaccccc aggctgcccc
    10141 gattcgtcgg cgaaggcagc cgtccactac ccactgaact ttccgtagtt cccaagccca
    10201 actcgccacc accagcccgc agtcgagcgg gcttttttgc gtagcttgca ccgatatgac
    10261 tcggaaaatt gtcatctgac cccaatttcc ccgcgtagcc ttctccctcg aactcgtcca
    10321 ggcgcgccca gtgcgccggc aactcgggcg aactgaagat gaggcccgga accccccgcc
    10381 ttcgactgac agcacgatgc ccggataacc cagcgctgcc ccccaaccct ccgggaacag
    10441 cgttccccga accgtagccg gctcccaggt gcccgggacc gtcgcaagaa cgtgcgcgtt
    10501 ggaccggcct ggtgcgaggg tgccgtagac gaacaggcgg tcaggcatgg cgtttcgaat
    10561 ggtagatcag gtcaatctcg actcgcctaa attttcatct gaccccaatt tccccttgat
    10621 ctgatcgcag tctcccatga atcagttcag gtgttgtttg aattgacaac agttcgctac
    10681 gttgtcagaa tcaccaacat gaaggccaca ttgctcctca aatcccgcga ggtattgagc
    10741 acgacggctt tcgtggagcg tgtgctgtgg ttgttgcccg aaccgctgtc gggcagcacg
    10801 caccgactca agtaccgcct ggcctttgtg gtggatggcg tctgcgtcgt gcgttacgac
    10861 aacgagcgcg gcaaaggcga ccatcggcac gtgggcgatt tggaatcgga ctatcccttc
    10921 agtacgcccg aggtgctgct gaacgacttc tggcgcgacg tggcgcagtg gaggcaaacc
    10981 catgaacacc gtgacgattg aactgaccga ccgccagacg caggacgcgc gcttcctgca
    11041 ggcgttcaag accggccggc cccagggcgc gtcgattgct ttcgattcgg tcgaagtcct
    11101 gtggcaggtg ctcacgccca agcgctggca gatcgttcgc accctgtgcg gcgcaggccc
    11161 gatgtcgctg cgcgaagccg cccgccgcgt gggccgcgac gtcaaggcag tgcacggcga
    11221 cgtgcatgcc ctgctgacgg ccggcgtgct ggagcgagac gctgcgggcc gaatcgtgtt
    11281 tccgtacgac gccgtgcacg tggatttcac ggtgcagccg gagaaggcgg tggcttgaag
    11341 gttccgacac agttgccgag gggaaattgg cagctgaccc gacgcatggg caccagcaat
    11401 tcttcaagcc aaattggcct gcaacgcttt accagcaagc gcgagcagct atcagttcga
    11461 tagtgactga ctttgccgcg attgccctgc cggggagtac aggcccttgc aaaagtgaaa
    11521 gggatgccga tccgcgaagc ggccgaggtt gtcgaggacc gtgacgatac ggcacgatgc
    11581 agttccgccg ttcttctcgc cgcgcaaccc ccggccgacc atctgcatgt agcgcacggg
    11641 gctgaagacc tggcgtgcaa tcaggaccat gtcggtcctg ggtgcgtcga aacctgtggt
    11701 cagtacgctg tgattgcaga gcacgcgaat tttcccgttc tggaagcgat caaggaagta
    11761 gcgccgcgca gcgttcggcg tgtcgccgct gatggctgcc gcgccgatgc ccgagagatt
    11821 caggcgtgcg gccatctctt ccgcatgggt gacggagttg gtaaagaaga ggatcgagtg
    11881 ttcagcactg gcccttatgc actccaccaa tcgctcgttg cgctgggcat cgtcagccag
    11941 acgttggttg atcgactcca gcaggttctc gaaatcaagg ccctcccaag gctcgcgcag
    12001 ttgtccgagc ttgtgcagtt cctcgtctgt cagatcagcg ccggtttcaa gcgattcata
    12061 ttgcgcttgc gcgagtacgc cttgcgcacg caggcgcgca tagagctgct cctggttaga
    12121 gggtagccaa cggttgtcga accggcgcgc taggcgctgg ctctcttcgt catctgtgcg
    12181 gaatggcgtt gcgcttaggc ccacgatcgg cggttcatcc ttgggggtcg cgccgggtct
    12241 gggtgcctca gcatccaacc accgcagcaa ctcgctgtag ctgggcgtga tggcatggtg
    12301 gcattcatcg atgaccacaa ggccaagctt gcgcagccag gcgagttccg cctggctgat
    12361 gcggctgttc agcgtctgaa tggacgctac aacgacaacc ggtttatccg agtccgatgg
    12421 cgttgggttg cggtttccgc cccaaaggcg ggcgatgcgt aagcttgttt tctgggcgcc
    12481 gagattgacc cagacctgac gaaacgcctg taccgcctgc tcgcacaact cgtcagtctg
    12541 ggcgatccag aggacgctgc gtgtcgtgtt ctccggtgcg agcacgagtt gtacggctgc
    12601 ctgtacggtc acgcgggttt tgcccccgcc tgtgggcaag ctgacaacag cccgccgtcg
    12661 ttgcgttccg ttcgcaagca gagatctcaa gccgtcgaac acctcctgtt ggaaggcgtg
    12721 tagtggtggc aattcgatcg gtccactcac caactcctcc ggctcgcgcc gggcttgaga
    12781 tgccgacgcg aattcaggag gaaagccgat ggctgccacg aacccgagtg catctgcgct
    12841 gttccagcgc gcgggtggtt gtagcccttc atcccgcagc gtgtctttca aggtgctcag
    12901 cgcggccggc cccagttgag cgagcaccag atcggcgagc tggttaggcg tgcattggcg
    12961 aacgaagtcc acgtcgcgca gccgctggcc caaggcgtcc agcagcggtt cgacccgatt
    13021 gcccgctgcg cgcagcaagc gttcggccaa cgtcgcgccc tctctgacgt atgtacgaca
    13081 tttgtcgaca ccagtgtcgc cgaggcgggc aagcgcatcc tcaggcgagt cgtcgagcca
    13141 gccggatggg gcgatctcag tgaacagccg cttcagtcgc tccgcccgcg agagcggtgc
    13201 cagttgctca gcatccagga acaatacgcc gtctcgcatc aggcaaggag ccgcttgtgc
    13261 cgagccggcg atgcccaggc agagtttggt cacgcgctgg cagcgcgctg cgtcgcgaat
    13321 atctgctcgc aagacatcgc ccagctcagg cattacatct atcaggcgct caagtgggcc
    13381 tgcgatgtcg ctccattccg ttcggatcag gctcgcgaga tcttgcgcgc cagccttctg
    13441 ccagagctgc atcgtaggtt gatcgagtac cacgaccacg cgctctggag tccgagcgcg
    13501 ctgtgccaga tcggcggagg tggttacgaa tgccgcgctc agtggaacgt ggccggtcag
    13561 cgaaggtagt tcgattggta cgacgccatc cttggcggcg ccgagccaga ggtcggttaa
    13621 cacatcgctt tcaagcgagt ggggggtgat gattgctttg aacattgcaa cccaaaaggt
    13681 gcgcaggtcg gattgcgtca ccgtcacctc agggaagtca acattcaaca ggtgaagctt
    13741 cgcggcggtg cactcccact ccggtagatg tttcaggatc ggttgatcgc ggcgtaggaa
    13801 gacggctttg aggggtgccg agagcgtgcc tatctgcaga gcgccatgat cgtgcacgta
    13861 ccacagcaac ggatggggta cgtcgatctt tgggtagacc gacattgtgc tatggccaaa
    13921 ggggatggat ttttgatagg caggttccgc gagacgattc agtagttgat ttgtataacg
    13981 ggtctttggg acgccggtta gttcagttag cagtctgaaa ccccgtggca tggagaacgg
    14041 atagggcttg aggtagtgcc aagaagcaga attctgatgc gtctccttgt agcggctgcg
    14101 acactcttca agccatggat ttagccaagt ttcgcccgta aactcaggga atcggatggg
    14161 tccaacgtcg cccgcaggca cacgcaacac acccagagat tccaacgacg ctcgatcctt
    14221 tgcgtgcgta tcaggatcga cgagtatgtt cacatttggc accgattcgc catcacttac
    14281 caactcgcca gggaatacga cgcgatccgc tttcacccaa cttccatcgc ggcggcgcac
    14341 gcgcacactc tcgctgtgcg tgcctaggaa ccgattgcgt gctcctgctg gcgcattgcg
    14401 taaacattcc cagaatgttt cccaaccctc ttcttcggtt atgctggacc agttcgaact
    14461 cggcagagcc tcaccgagga tcgatatcca cactgcgtcg tccaaatcac ggacaagaag
    14521 cgcacttctc agaatctccc gcgtctccgg gttgaattgg agttcggcgg tgatcaaatc
    14581 ccgaccgggt acgggtacgc ccaccggagt gaagacaacc tgggacgccg tggccagctt
    14641 acctgactga gtgggaatga ttgtcagagt agggcgaatt tcgccccact cgtcagcgtt
    14701 tactcgggag aacgccttcg ccagatgtag gacaccgatc gcaacgtcgg tctgggttga
    14761 tgcaactgcg cgaaaccaag cggcaggtct gcagcggcga agttccggag aggtttgttc
    14821 agtcgtttgc gcctcgatgc gctccgccag cgcattcaac cggctggcgc gctggcgctg
    14881 cagacaggat gcatgtatca actggcgctg ctgctcagga cccgcgagtg actgccattc
    14941 aactgctagc gcaggatcgt catttgggtg gcgccaaagc tcatttgcgc ttcgcagagc
    15001 gccggtgccg tctgatatga cgacggccgt ctccaatcga gcccaaagtg actgaacgag
    15061 tggtactgca atctcgtctt tccgttccag ttgacgggga aaggcatcga gcggccgtgc
    15121 agggtcatcc gacgtagcca agttaggcat catctcggcg attaagctcg ctgcctcggt
    15181 catcagcgcc gcattccact cgccaccaat gatggcattg cggtcactgt tcagcttcca
    15241 cggcgcattc aagattccag gcaaataggt ctgcgtatgt gttgggaaga aggcccagaa
    15301 ccggccggac tcttcgcgac gtccttcgag cggcatggcc catgaaaggg gcacgcattc
    15361 acgagcgtga atgtgcgtag cgtctgcaac ggcacgcgat tcagtgatac gaacgtcacg
    15421 cgtgatgatg ttccacagtg aagtggcctc cccatcctgc aggatggttt cgccacgatg
    15481 cttgaccacg cgaactgtac gtatgttttc ctcgccaaaa tcaagagtta gcgtgaccgc
    15541 aatcggaaga aacagcagga actcagccgg aaatccccga atttcgtttt gcaggtgcag
    15601 taatagttct gctgaatcga cttcggcgcg aacgatcgtc tcggcccatg caagctcagc
    15661 gcaaattgga tccttggcgc gctcgtctgg tggcagaggc catacgagcc gcaagccagg
    15721 tgcctgtttc acgttaaatt ggcgacgaag ttcgtctctg cacctttctg gatcgaaccg
    15781 aatcgctccc gaagaccgcg taaacacatc aatctgtccg ttgagcttca ggagtgactt
    15841 gaagcccaat ccgaagcgac caatttggtt gcggcgcttc ggtgacgaat gtgatcgaag
    15901 aagcgcatcc aagccttctt cactcagcgg cgcaccggtg ttggcgacat agagtcgcga
    15961 tccatgtaac tggacgtgga tacgcccgtc actggactga gggccgccac tctctttggc
    16021 ttccagcaat gcgtcggcac cgttttgaac gagctcaaga atttgccgat agccgtaacc
    16081 gcctgctaac accgtctcct cgataccggc gtgctctctc agtaggccag agtccgcttc
    16141 gtaggagcgg aggcggttat tgttgaattg ctgaagtgca gcatggagca cgaatcgaac
    16201 ctcctcattt gcttgagcgt cagcccatca attcctcata ccttgcttcc ccccgccaat
    16261 ctccgataca tcgtcgcccg gctcaccccc agcgccttgg cggcgacggc gacgcgcccg
    16321 ccggcgtcgc gcacggcctg gtggatcagc tcggtttcca gcgctttcag tg
//
LOCUS       DAOOOG010000038        15805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_3927_length_15805_cov_31.740381, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000038 DAOOOG010000000
VERSION     DAOOOG010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 15805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15805
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3927_length_15805_cov_31.740381"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PK242_04790"
     CDS             <1..448
                     /locus_tag="PK242_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOZ93365.1"
                     /translation="INYIRRNYGMLIGDPTAELIKKEIGSAFPGSEVREMEVKGRNLS
                     EGVPRSFTISSNEILEALTEPLNNIVSEVKKALEETPPELGADIAERGMMLTGGGALL
                     RDLERLLAEETGLPVLVAEEPLTCVVRGCGLALERMERLGSIFTSE"
     gene            629..1648
                     /gene="mreC"
                     /locus_tag="PK242_04795"
     CDS             629..1648
                     /gene="mreC"
                     /locus_tag="PK242_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOZ93366.1"
                     /translation="MPLDTLDRTPPPFFKQGPSALSRLVFFSALALFLMVADARFHIV
                     QPLRAAIATVLYPAQWLALQPVQMVRQLTGYMTDLKTARATEEAARRKLAEQSERAGQ
                     VEQLLLENTRLRELLELRQRVQAPGMAAQVLYDATDPYSRRVVIDKGQLQGVQPGSPV
                     LDESGVLGQVTRVYPFVSEITLLIDREQVIPVMNARTGTRSVAYGDPAPHGGAMELRY
                     MSVNEDVKEDDLLVTSGVDGVYAPGLPVARVTTVDRRSDTSFARIRCQPLARLQGVTH
                     VMVLAPLTPQVAEGPTGAATADSGAPTAASAPAQASAPASAAASAAASAPAAPRRRAA
                     TGGRP"
     gene            1645..2163
                     /gene="mreD"
                     /locus_tag="PK242_04800"
     CDS             1645..2163
                     /gene="mreD"
                     /locus_tag="PK242_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOZ93367.1"
                     /translation="MILPRGQPLLLPAHPGFIWGSLLLALMLDLLPLGRVPWMPDFLA
                     VVLVFWSIHQPRRVGLSAAFVFGLVLDVHQASLLGQHALAYAVLIYLAIMVHRRILWF
                     AVPAQALQMLPLFAAAHAITLVLRLIGGGIFPGWSVLLAPLLEALMWPIVSWLLLAPQ
                     RRAPNPDANRPL"
     gene            2345..4297
                     /gene="mrdA"
                     /locus_tag="PK242_04805"
     CDS             2345..4297
                     /gene="mrdA"
                     /locus_tag="PK242_04805"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOZ93368.1"
                     /translation="MTELRNVEAELMRFRIRLAVVALAVLLAFGLLGWRLYQLQVARH
                     DELARQAESNRTAVVPVVPHRGQITDRHGVVLATNYKAYTLEITPSRTGRGIDDTIDA
                     IAQVIEVTPRDRRRFKRLREDSRSFESLPIRTRLTDEEVARFAAQRYRFPGVEIKARL
                     FRNYPLGETGAHVIGYIGRINPKEKERIEDSDDAANYRGTDHIGKLGIEASYEKLLHG
                     TTGWEQMETSAGGRAVRRLGSHPATPGEAIRLTIDIHLQKLVEDMYGERRGALVAIDP
                     TNGEVLAFVSKPSFDPNLFVEGIDHESWDELNNSIDKPLLNRGLRGTYPPGSTYKPFM
                     GLAALETGKRAPGSVTMDGGSWTFGGHTFRSGHALGPVDLHRSIVKSSNVYYYQLAND
                     MGVNAIHDFMKPLGFGQITGIDLPGEARGVLPSTDWKRTTYRRPEQQKWYAGETISLG
                     IGQGYNNFTILQLASATATLAAGGVRHVPHLVKARQDAVSLQLHELPQPAAQPLGYRP
                     EHVALVRRAMVGVTQEGTSRAVFAGAPYLSGGKTGTAQAVTIGQKSRYNAAKLAEHQR
                     DHSLYIAFAPAEQPRIAVAAIVENAGFGAAHAAPLVRRVMDYWLAGVYPSEADIAALQ
                     RGQASAPIGTPRRVAEVTAAAPSAAP"
     gene            4680..6209
                     /locus_tag="PK242_04810"
     CDS             4680..6209
                     /locus_tag="PK242_04810"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOZ93369.1"
                     /translation="MSQDSRRCPVTHLTTDFGAPVPTNRDSLTAGPRGPLLAQDVWLN
                     EKLANFVREVIPERRMHAKGSGAFGTFTVTHDITRYTRAKIFEKVGKKTEMFARFTTV
                     AGERGAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFIRDPRQFPDLNKAVKRDPRT
                     NMRSATHNWDYWTLLPEALHQITIVMSERGIPASYRHMHGFGSHTYSFWNAKGERFWV
                     KFHFKTQQGIKNLTDAEAEALIGRDRESHQRDLYEAIARGDFPKWTMYVQVMPELDAD
                     KVAYHPFDLTKIWPHADYPLIEVGEFVLNKNPENFFADVEQSAFAPSNLVPGIGVSPD
                     RMLQARLTNYADAQRYRLGTNHHQIPVNAARCPVHSNHRDGMGRVDGNYGGALHYEPN
                     SFGQWQAQPQFAEPPLRLSGDAQHFRFHEDDANYFEQPRKLFQLMSDAQKQALFGNTA
                     RAMGDAPEFVKQRHIRNCHAADPAYAAGVAQALGLDLQKALASQKDDPMFGNPLVALP
                     V"
     gene            6254..6736
                     /locus_tag="PK242_04815"
     CDS             6254..6736
                     /locus_tag="PK242_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HOZ93370.1"
                     /translation="MNTPRINIGISEQDRAAIAQGLSRLLADTYTLYLTTHNFHWNVT
                     GPMFNTLHAMFMAQYTELWNAVDPIAERIRSLGHVAPGSYAAFGKLSSLADAPPEPPR
                     AMDMVRLLVQGHEAVARTARELFPQADAAGDEPTADLLTQRMTVHEQTAWMLRSLLEE
                     "
     gene            complement(6830..6905)
                     /locus_tag="PK242_04820"
     tRNA            complement(6830..6905)
                     /locus_tag="PK242_04820"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6870..6872),aa:Arg,seq:ccg)
     gene            complement(6925..7575)
                     /locus_tag="PK242_04825"
     CDS             complement(6925..7575)
                     /locus_tag="PK242_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOZ93371.1"
                     /translation="MARRTKEDALATRAALLDAAECVFQQRGVSRTSLADIAQAAGVT
                     RGALYWHFKDKAALFNAMMDRVTLPLGAELEGLLEQRDDPLQALCQHLDEALHQIVHD
                     EQTRRVLRIAMQKVEHAEELGPVIDRHIQMHRLNTERERQVFERAAALRQCVLPAPAA
                     QLAHGFYAMVHGLIYSWLLDEGFDLQATAAVSVQAFLRGIGLVPAQAAPSPAADGR"
     gene            7817..9106
                     /locus_tag="PK242_04830"
     CDS             7817..9106
                     /locus_tag="PK242_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ93372.1"
                     /translation="MTSKQTVSRTWARRLAPVGIAAALTLTLAACGKGQPPAAGAGGP
                     GGGMPPPEVAVLTVQPGVVPISTELPGRLEASRVAQVRARVTGILQRRVFTEGSDVKA
                     GQTLFRIDAAPYEATLQSARAQLAQAQAQLAQTSATAARYKPLVAANAVSRQEYDAAV
                     AAEKAAQAQVAAGQAAVRTASINLGYATVTAPIAGRIGRALVTEGALVNQQQDGGTPL
                     AVIQQIDPLYINITQSANQVLQLREALAAGQLARASGAEAAKVQVLLDNGQPFGQSGR
                     LLFTDLSVDPATGEVSLRAELPNPGGRLLPGMYVRVRIEQAQVEGAILLPQQAVTRGS
                     QGDSVLVVAADGSVAPRPITIRGAQGQHWIVTGGLQPGEQVMVEGAMKLMLGAKVVKP
                     VPWQPKSAAAPDGQAPAATKTEATKAPASAPAGAASN"
     gene            9119..12373
                     /locus_tag="PK242_04835"
     CDS             9119..12373
                     /locus_tag="PK242_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ93373.1"
                     /translation="MARFFIDRPIFAWVIALFLMVIGATAITQLPIAQYPPVAPPTIV
                     VNAAYPGASAQTLEDSVLAVIEREMNGAPGLAYMESVAQADGTGQITISFEPGTNADL
                     AQVEVQNRLSRATPRLPAVVNQQGVRVDKARANFLMFVMLSSSNPDVDVTGLSDYAAR
                     NVQPELQRVPGIGQVQLFGSERAMRIWIDPDKLHSYKLAPSDVASAIQAQNAQVSAGA
                     IGNQPLVAGQTMTATVVVPGQLSTPEQFGAIVLRANADGSSVRLKDVARIELGAQSYA
                     SAARLNGEPGAGMGIQLSPTGNALAAAEAVRARMSELQAFFPPGVTWTIPYDASNFIK
                     ISIRKVVETLLEAVVLVFLVMFLFLQNWRYTLIPTIVVPVALLGTFGALLAFGMSINV
                     LTMFGMVLVIGIVVDDAIVVVENVERIMSEEGLPPLAATRKAMSQISGAIIGVTAVLI
                     AVFVPLAFFTGSVGNIYRQFSAVMGVSIFFSAFMALSLTPALCGTLLKPVQAGHQQAK
                     GGFFGWFNRGFARTTRGYEGVVARLLRRSGRLMLIYLLLAAVVGWMYVRLPTAFLPQE
                     DQGNLLVNIQLPPGATTERTQTVVEQIEGYLLKQPEVQSMVAVMGFSFSGQGQNAGIS
                     FVTLKPWDERTGPQHAADAVAGRAMGALSTIKDAFIFPLNPPPIPELGVSSGFSFRLQ
                     DRGGKGHDALLAARNQMLGMAMQSKLLAGVRPNGLEDAPQLQIDIDRDRAAAQGVSFG
                     AINSVISASLGSSYVNDFPNAGRLQRVVVQADGPARMTPDDVLRLTVLNNKGQAVPLS
                     ALASTRWVTGAMQTVRYNGYPAMRIDGGAAPGYSSGEAMAEMERLASRLPEGFGFEWT
                     GQSREEKLAGNQALVLYGFAILAVVLALAALYESWSIPLAVILVVPLGVLGVLLAVTG
                     RGLLNDVYFQVGLITIIGLSAKNAILIIEFAKDLQAQGKSALESALAAAHLRFRPIVM
                     TSLAFGLGVLPLAIASGASSASQRAIGTGVMGGIVVGTTLAVFFVPIFFVVVRSLFKP
                     NQRELERSQQHAEHAGITAASADAYVAAAEQGMSEAERQALHQGAQPPLTKDPS"
     gene            12370..13878
                     /locus_tag="PK242_04840"
     CDS             12370..13878
                     /locus_tag="PK242_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOZ93374.1"
                     /translation="MSHARHRLSRAPLVLAGLVAVGLLAGCSLVPPYERPAAPIASHW
                     PDTAGAPGADTTTAADLPWQQFVRDDQLRELIGLALTSNRDLRVAVQNIEQARAQFQI
                     RRADQFPSVGASASGTRSAPNPYQALGGPSVSSSYSVGLGVSAWELDFFGRVAALKDA
                     ALASYLATEEARKSSQISLIASVASTWLQLKTDTELLALAERTLGTREQSLALTRLRF
                     EHGAASALDLRQAETLIANARATRAQQQRLRAQDIHLLTLLVGQPLPERLIPAVPALP
                     TPQSPSEPTQASATVAPAAELPAFADVPAGLPSDVLLRRPDIRAAEQQLIAANAQIGA
                     ARANFFPRITLTGSLGRVSSDLDGLFGSGGSRAWSFGPAITLPIFDAGRNQAGLDSAL
                     AGRDIAVAQYEKAIQTAFREVADALAGRATLAEQLAALQAQAEAERARFQLTDLRYRN
                     GVASSLDLLDAQRSLFAIEQALAQARLAQRANEVQLYKALGGGWSESPAAGA"
     gene            14014..14877
                     /locus_tag="PK242_04845"
     CDS             14014..14877
                     /locus_tag="PK242_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ93375.1"
                     /translation="MSDSTHDDAHTGPIKSPKQMFWVSVAAFVLPVFIIIGLVSFVTS
                     GNKTSPGASDMERAIAERLQKVGRVEIRDANRPLASGEEVYKTQCAACHATGVSGAPK
                     FQNAGDWGSRLGQGYAALLNSSLKGKGAMAPQGGGNFSDLEIGRAVVYMANAAGGKLA
                     EPAAPAAAASAADAAPAPAAAAAPAPAAAPAPAPVAAAAPAPAAAAGDAAAGKALYDK
                     ACVTCHAAGVAGAPKLGDKAAWAPRIAEGMDAMMKIAINGKGAMPPRGASTASDDELR
                     AAVQFMVDAAK"
     gene            complement(15246..15788)
                     /locus_tag="PK242_04850"
     CDS             complement(15246..15788)
                     /locus_tag="PK242_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HOZ93376.1"
                     /translation="MDDIVRQAMAKWPNVPHCHGWLGLDARGHWYLRDAQAQALGGFA
                     SGVAGARGTRLQHEGLIGFIQRNYAADAAGCWYFQNGPQRVFVELEVAPWVWRVASDG
                     AVSAHDGRPALVQAVWLDEQGRVFLRTDLGFGIVHTLDMGAVADAVEAGDWQPVAVHS
                     AELPQREGYVLSPQARSCRP"
BASE COUNT         2528 a         5769 c         5251 g         2257 t
ORIGIN      
        1 catcaactac atccgccgca actacggcat gctgatcggc gacccgacgg ccgagctgat
       61 caagaaggaa atcggcagcg ccttcccggg cagcgaggtg cgcgagatgg aggtcaaggg
      121 ccgcaacctg tccgagggcg tgccgcgcag cttcaccatc agcagcaacg agatcctcga
      181 agcgctcacc gagccgctca acaacatcgt ctccgaagtc aagaaggcgc tggaggaaac
      241 cccgcccgag ctgggcgccg acatcgccga gcgcggcatg atgctgaccg gcggcggcgc
      301 cctgctgcgc gacctggagc gtctgctggc cgaggaaacc ggcctgccgg tgctggtggc
      361 cgaggagccg ctgacctgcg tggtgcgcgg ctgcggcctg gcgctggagc gcatggagcg
      421 gctgggcagc atcttcacca gcgaatgacg ccgcgtcccg gtggccgcag gtggccggcg
      481 cgccgccacg ccccggcctg ccgccgacgc cgcccgcacg ctccgccgct tcatgcaaaa
      541 actgcttctg gcgctttacc ggcgtgcgca aatagctctt attaatgtag catcgcgtct
      601 gtcccgaccg atgtcggcct gacccgccat gccgctcgac acgctcgacc gcacgccgcc
      661 gcccttcttc aagcaagggc cgtcggcgct gtcgcggctg gtcttcttca gtgcgctggc
      721 gctgttcctg atggtggccg acgcgcgctt tcacatcgtg cagccgctgc gcgcggccat
      781 cgccaccgtg ctctacccgg cccaatggct ggcgctgcag ccggtgcaga tggtgcggca
      841 gctgaccggc tacatgaccg acctgaaaac cgcgcgcgcc accgaggagg cggcgcggcg
      901 caaactggcc gagcagtccg agcgcgccgg tcaggtcgag cagctgctgc tggaaaacac
      961 ccgcctgcgc gagctgctgg agctgcgcca gcgcgtgcag gcgccgggca tggcggcgca
     1021 ggtgctgtac gacgccaccg atccctacag ccggcgcgtg gtgatcgaca agggccagct
     1081 gcagggcgtg cagccgggct cgccggtgct ggacgagtcc ggcgtgctgg gccaggtcac
     1141 gcgcgtctac cccttcgtca gcgaaatcac cctgctgatc gaccgcgagc aggtgatccc
     1201 ggtgatgaac gcacgcaccg gcacgcgcag cgtggcttat ggtgacccgg cgccgcacgg
     1261 cggcgccatg gagctgcgct acatgtcggt caacgaggac gtcaaggaag acgatctgct
     1321 ggtgaccagt ggcgtggacg gcgtgtatgc gcccggcctg ccggtggcgc gcgtgaccac
     1381 ggtggaccgg cgcagcgaca cctcgtttgc ccgcatccgc tgccagccgc tggcgcggct
     1441 gcagggggtg acgcacgtga tggtgctggc gccgctgacg ccccaggtcg ccgaggggcc
     1501 gaccggcgcc gccaccgccg actccggcgc gccgacggcc gccagcgcac ccgcccaggc
     1561 ctcggcgccc gcttcggcag ccgcctcggc agcggcttcc gcccccgccg cgccgcgccg
     1621 gcgcgccgcc accggaggcc gcccatgatc ctgccgcgcg gtcaacccct gctgctgccg
     1681 gcccaccccg ggttcatctg gggcagcctg ctgctggcgc tgatgctcga cctgctgccg
     1741 ctgggccggg tgccatggat gcctgacttc ctggcggtgg tgctggtgtt ctggagcatc
     1801 caccagccac gccgcgtggg cctgagcgcg gccttcgtgt tcggactggt gctggacgtg
     1861 caccaggctt cgctgctggg ccagcacgcg ctggcctacg cggtgctgat ctacctggcc
     1921 atcatggtgc accggcgcat cctgtggttc gcggtgccgg cgcaggcgct gcagatgctg
     1981 ccgctgttcg cggcggcgca cgccatcacc ctggtgctgc ggctgatcgg cggcggcatc
     2041 tttccgggct ggtcggtgct gctggcgccg ctgctggagg ccctgatgtg gccgatcgtc
     2101 agctggctgc tgctggcgcc gcagcgccgc gcgcccaacc cggacgccaa tcgcccgctg
     2161 taggtggttt cgtggtcaaa taccgctttc gcgcttatct ggcaagcgca agcagctatg
     2221 catttaatag cattcgggac atgatggcac ggcactggag gcgcgcgacc ctggcacacc
     2281 gggccccggg cggcccacac gctcggcggc acggcgggcc tgcgcccgcg aggcaggcgg
     2341 caccatgacc gaactgcgca acgtcgaagc cgagctgatg cgctttcgca tccggctggc
     2401 ggtggtcgcg ctggcggtgc tgctggcctt cggcctgctg ggctggcgcc tgtaccaact
     2461 gcaggtggcg cgccacgacg aactggcgcg tcaggccgaa tccaaccgca cggcggtggt
     2521 gccggtggtg ccgcaccgcg gccagatcac cgaccgccac ggcgtggtgc tggccaccaa
     2581 ctacaaggcc tacacgctgg agatcacacc ttcgcgcacc ggccgcggca tcgacgacac
     2641 catcgacgcg atcgcccagg tgatcgaagt cacgccacgc gaccggcgcc gcttcaagcg
     2701 cctgcgcgag gattcgcgca gcttcgaatc gctgccgatc cgcacccgcc tgaccgacga
     2761 ggaggtggcg cgctttgccg cccaacgcta ccgcttcccc ggggtcgaaa tcaaggcgcg
     2821 gctgtttcgc aactacccgc tgggcgaaac cggcgcccat gtgatcggct acatcggccg
     2881 catcaacccg aaggaaaagg agcgcatcga ggattcggac gacgcggcca actaccgcgg
     2941 caccgaccac atcggcaagc tcggcatcga ggccagctac gaaaagctgc tgcacggcac
     3001 caccggctgg gagcagatgg agacctcggc cggcggccgc gccgtgcgtc gcctgggcag
     3061 ccaccccgcc accccgggcg aggccatccg gctgaccatc gacatccacc tgcaaaagct
     3121 cgtcgaagac atgtacggcg agcgccgcgg cgcgctggtg gcgatcgacc cgaccaacgg
     3181 cgaagtgctg gccttcgtca gcaagccgag cttcgacccc aacctgttcg tcgaagggat
     3241 cgaccacgaa agctgggacg aactcaacaa ctccatcgac aagcccctgc tcaaccgcgg
     3301 cctgcgcggc acctacccgc ccggctcgac ctacaagccc ttcatgggac tggcggcgct
     3361 ggaaaccggc aagcgcgccc cgggcagcgt caccatggac ggcggctcct ggaccttcgg
     3421 cggccacacc ttccgttccg gccacgcgct cgggccggtg gacctgcacc gcagcatcgt
     3481 caagtccagc aacgtgtact actaccagct ggccaacgac atgggcgtca atgccatcca
     3541 cgacttcatg aagccgctgg gttttggcca gatcaccggc atcgacctgc ctggcgaagc
     3601 gcgcggcgtg ctgccctcga ccgactggaa gcgcaccacc taccggcgcc ccgagcagca
     3661 gaagtggtac gccggcgaaa ccatctcgct gggcatcggc cagggctaca acaacttcac
     3721 catcctgcag ctggccagcg ccaccgccac gctggcggcc ggcggcgtgc gccatgtgcc
     3781 gcacctggtc aaggcccgcc aggacgcggt gagcctgcag ctgcatgagc tgccgcagcc
     3841 ggctgcccag ccgctgggct accggcccga gcacgtggcg ctggtcaggc gtgccatggt
     3901 cggcgtgacg caggagggca cctcgcgcgc ggtgttcgcc ggtgcgccct acctctcggg
     3961 cggcaagacc ggcaccgcgc aggcggtgac catcgggcag aagagccgct acaacgccgc
     4021 caagctggcc gagcaccagc gcgaccactc gctctacatc gcctttgcgc cggccgaaca
     4081 gccgcgcatt gcggtggcgg ccatcgtcga aaacgccggc ttcggggccg cccacgccgc
     4141 gccgctggtg cgccgcgtga tggactactg gctggccggc gtctacccga gcgaagccga
     4201 catcgcggcg ctgcagcgcg gccaggccag cgcccccatc gggacgccgc ggcgcgtggc
     4261 cgaagtcacc gccgccgcgc ccagcgcagc gccctgacgc cgccggcggc ccggccgccc
     4321 caacggcggc gcaccggccc gatgcctgcc ggtcaccgct gcccgaagcc accactgacg
     4381 cgccggcatg gcgtcgcgtg gctgtgctct ggcgcaccga tcgaatgcga gcgcctccaa
     4441 ctgcgttgaa cctagaaacc gcagttgacc gcttgcaacc cttggaggtg ccgcatgaac
     4501 tctgaccttg caccacttgg gagccttcac cttctgggtg aaggcgctat gcactgccgt
     4561 ttctaggttg aaccggccta atgccttgat tgaaactatt gattgttcat atcaatgcat
     4621 gcgacctatc atgacctcag tcatccaccg ccccacccat ttcctccaag gagtcaacca
     4681 tgagccaaga ttcgcgccgc tgccccgtca cccacctgac caccgacttc ggcgcccccg
     4741 tgccgaccaa tcgtgacagc ctcaccgctg gcccacgcgg cccgctgctg gcgcaggatg
     4801 tgtggctgaa cgaaaaactc gccaacttcg tgcgcgaggt gattcccgag cggcgcatgc
     4861 atgccaaggg ctcgggtgcg ttcggcacct tcacggtcac gcacgacatc acgcgctaca
     4921 cgcgcgccaa gatctttgag aaggtcggca agaagaccga gatgttcgcc cgcttcacca
     4981 ccgtcgcggg cgagcgcggt gcggccgacg ccgagcgcga catccgcggc tttgccctca
     5041 agttctatac cgaggagggc aactgggaca tggtgggcaa caacacgccg gtgttcttca
     5101 tccgcgaccc gcgccagttc cccgacctga acaaggccgt caagcgcgac ccgcgcacca
     5161 acatgcgcag cgccacgcac aactgggact actggacgct cttgcccgag gccttgcacc
     5221 agatcaccat cgtcatgagc gagcgcggca ttccggcgag ctaccgccac atgcacggct
     5281 ttggctcgca cacctacagc ttctggaatg caaagggcga gcgcttctgg gtcaagttcc
     5341 acttcaagac gcagcagggc atcaagaacc tcacggacgc cgaggccgag gcgctcatcg
     5401 gccgtgaccg cgagagccac cagcgcgatc tctacgaagc catagcacgc ggcgatttcc
     5461 ccaagtggac catgtatgtg caggtcatgc ccgagctcga tgccgacaag gtggcctacc
     5521 acccgttcga cctgaccaag atctggccgc acgccgacta cccgctgatc gaggtgggcg
     5581 agttcgtgct caacaagaac cccgagaact tcttcgctga cgtggagcag tccgcgtttg
     5641 cccccagcaa cctggtgccc ggcataggcg tgagccccga ccgcatgctg caggcgcgcc
     5701 tgaccaacta cgccgacgcg cagcgctacc gcctgggcac gaatcaccac cagatcccgg
     5761 tgaacgccgc gcgctgcccc gtgcacagca accaccgcga cggcatgggc cgggtggacg
     5821 gcaactacgg cggcgccctg cactacgagc ccaacagctt tggccagtgg caggcccagc
     5881 cccagtttgc cgagccgccg ctgcgcctgt cgggcgatgc ccagcacttc cgcttccacg
     5941 aggacgacgc caactacttt gagcagccgc gcaaactctt ccaactgatg agcgacgcgc
     6001 aaaaacaggc gctgtttggc aacaccgcgc gcgccatggg cgatgcgccc gagttcgtca
     6061 agcagcgcca catccgcaac tgccacgcgg ccgacccggc ctacgccgcc ggcgtggccc
     6121 aggcgctggg cctggatctg caaaaggccc tggcctcgca gaaggacgac cccatgttcg
     6181 gcaacccgct ggtggcgctg cccgtgtgag cggtcgccac cgtcaccatc ccatcgactt
     6241 caggagattc gccatgaaca cgccccgcat caacatcggc atcagcgaac aggatcgcgc
     6301 cgccatcgcc cagggcctgt cgcgcctgct ggccgacacc tacacgctgt acctgaccac
     6361 gcacaacttc cactggaacg tcaccgggcc gatgttcaac accctgcacg ccatgttcat
     6421 ggcgcagtac accgagctgt ggaacgctgt cgatccgatc gccgagcgca tccgctcgct
     6481 cggccatgtg gctcccggtt cctacgccgc cttcggcaag ctgtcgtcgt tggcggatgc
     6541 gccgcccgag ccaccgcgcg ccatggatat ggtgcgcctg ctggtgcagg gccatgaggc
     6601 cgtggcgcgc accgcgcgcg agctgttccc gcaggccgac gccgccggcg acgaaccgac
     6661 ggccgacctg ctgacccagc gcatgacggt gcacgagcag accgcctgga tgctgcgttc
     6721 cttactggaa gaataggcgc cagcgcccga ccagcgtgcg caagcagcta ttaactaagt
     6781 agcaatcaac aaaaaagccc tgacgctggc caaggctttc ttgatgatct ggcgcggctg
     6841 gcggggatcg aacccacgac cctcggcttc ggaggccgat actctatcca ctgagctaca
     6901 gccgcgtgca atgcaagccg ggcattatcg cccatcggcc gccggtgacg gcgcggcctg
     6961 cgccggcacc aggccgatgc cgcgcagaaa ggcctgcacc gacaccgcgg cggtggcttg
     7021 caggtcaaac ccttcgtcga gcagccagct gtagatcagc ccgtgcacca tggcgtagaa
     7081 gccgtgcgcc agctgcgccg ccggggccgg caacacgcac tggcgcagcg ccgccgcgcg
     7141 ctcgaacacc tggcgctcgc gctcggtgtt caggcggtgc atctggatgt ggcgatcgat
     7201 caccgggccc agttcctcgg catgctcgac cttctgcatg gcgatgcgca acacgcgccg
     7261 cgtctgctcg tcgtgcacga tctggtgcag cgcctcgtcc agatgctgac acagcgcctg
     7321 cagcggatcg tcgcgctgct ccagcaggcc ctccaactcg gcgcccagcg gcagcgtgac
     7381 gcggtccatc atggcgttga acagcgccgc cttgtccttg aagtgccagt acagcgcgcc
     7441 gcgcgtgacc cccgcggcct gggcgatgtc ggccagcgag gtacgtgaca ccccgcgctg
     7501 ctgaaacacg cactcggccg cgtccagcag cgcggcacgg gttgcgaggg catcctcctt
     7561 ggtgcgtcgg gccatgcaga aaaccggaaa aacgggaaga atcgctgctc gtcagcgatc
     7621 ggcacggcat ccattgtagc gcatacattc aagaatgtat gtataattcg cgccatcgtc
     7681 agttgtcccc gacgccgtgc cggcgccgcc cccagaagtg gtgccgcccg ctcaaccgct
     7741 tcggtggccg ccgctcatca tggcgtggcg ctgacggccg tgcctgccga tccctccgtc
     7801 gcaaagaaca agcctcatga cctcaaagca aaccgtctcc cgcacctggg cgcgccgcct
     7861 ggcgcccgtc ggcatcgccg ccgcgctgac cctgacgctg gccgcctgcg gcaaaggcca
     7921 gcccccggcc gccggcgccg gcgggccggg cggtggcatg ccgccgcccg aggtggcggt
     7981 gctcactgtg cagccgggcg tggtgccgat cagcaccgag ctgccgggcc ggctggaggc
     8041 ctcgcgcgtg gcccaggtgc gcgcgcgcgt caccggcatc ctgcagcggc gcgtgttcac
     8101 cgaaggcagc gatgtgaagg ccgggcagac gctgttccgc atcgacgccg ccccctatga
     8161 agcgacgctg cagagcgccc gggcccagct ggcgcaagcg caggcgcagc tggcgcagac
     8221 cagcgcgacc gctgcgcgct acaagccgct ggtggcggcc aacgcggtca gccggcagga
     8281 atacgacgcc gccgtggcgg ccgaaaaagc cgcgcaggcc caggtggcgg ccggtcaggc
     8341 ggcggtgcgc acggccagca tcaacctcgg ctacgccacc gtgacggcgc cgattgccgg
     8401 ccgcatcggc cgcgcgctgg tgaccgaagg cgcgctggtc aaccaacagc aggacggcgg
     8461 cacgccgttg gcggtgattc agcagatcga tccgctctac atcaacatca cccagtcggc
     8521 caaccaggtg ctgcagctgc gcgaggccct ggcggccggc cagctggcgc gcgccagcgg
     8581 cgccgaagcg gccaaggtgc aggtgctgct ggacaacggc cagccgttcg gccagagcgg
     8641 gcggctgctg ttcaccgacc tcagcgtcga ccccgcgacc ggcgaagtca gcctgcgcgc
     8701 cgaactgccc aacccgggcg gccggctgct gcccggcatg tacgtgcggg tgcgcatcga
     8761 gcaggcgcag gtggaaggcg ccatcctgct gccgcagcag gcggtcacgc gcggcagcca
     8821 gggcgactcg gtgctggtgg tggcggccga cggcagcgtg gcgccgcgcc cgatcaccat
     8881 ccgcggcgcc cagggccagc actggatcgt caccggtggc ctgcagccgg gtgagcaggt
     8941 gatggtcgaa ggcgccatga agctgatgct gggcgccaag gtggtcaaac ctgtgccatg
     9001 gcagccaaaa tcggctgcgg cgcccgatgg acaagcgccg gcagctacca aaacagaagc
     9061 aaccaaagcc ccggcctcgg caccggcggg cgccgcgtcg aactgagagg cgcccggcat
     9121 ggcacgtttt ttcattgacc gacccatctt cgcgtgggtg attgccctgt tcctgatggt
     9181 gatcggcgcc accgccatca cccagctgcc gatcgcgcag tacccgccgg tggcgccgcc
     9241 gaccatcgtg gtcaacgccg cctacccggg cgcctcggcg cagacgctgg aagactcggt
     9301 gctggccgtg atcgagcgcg agatgaacgg cgcgcccggc ctggcctaca tggaatcggt
     9361 ggcacaggcc gacggcaccg gccagatcac catcagcttc gagcccggca ccaacgccga
     9421 cctggcccag gtcgaggtgc agaaccgcct gtcgcgcgcc accccgcgcc tgcccgccgt
     9481 ggtcaaccag cagggcgtgc gggtggacaa ggcgcgcgcc aacttcctga tgttcgtgat
     9541 gctgtcctcg tccaacccgg acgtcgacgt caccgggctg tccgactacg ccgcgcgcaa
     9601 cgtgcagccc gagctgcagc gcgtgccggg catcggccag gtgcagctgt tcggttccga
     9661 gcgcgccatg cgcatctgga tcgacccgga caagctgcac agctacaagc tggcgccgtc
     9721 cgacgtggcc agcgccattc aggcgcagaa cgcgcaggtg tcggccggtg ccatcggcaa
     9781 ccagccgctg gtggcgggcc agaccatgac cgccaccgtg gtggtgccgg gccagctgtc
     9841 cacgccggag cagttcggcg ccatcgtgct gcgcgccaac gccgacggct ccagcgtgcg
     9901 cctgaaggac gtggcgcgca tcgagctggg ggcgcagagc tacgccagcg ccgcgcgcct
     9961 gaacggcgaa cccggcgccg gcatgggcat ccagctgtcg cccaccggca acgccctggc
    10021 ggcggccgag gccgtgcgcg cgcgcatgag cgagctgcag gcgttcttcc cgcccggcgt
    10081 gacctggacc atcccctacg acgcctccaa cttcatcaag atctcgatcc gcaaggtggt
    10141 cgagacgctg ctggaagcgg tggtgctggt gttcctggtg atgttcctgt tcctgcagaa
    10201 ctggcgctac accctgatcc ccaccatcgt ggtgccggtg gcgctgctgg gcaccttcgg
    10261 cgccctgctg gcgttcggca tgtcgatcaa cgtgctgacc atgttcggca tggtgctggt
    10321 gatcggcatc gtggtggacg acgccatcgt ggtggtcgag aacgtcgagc gcatcatgag
    10381 cgaggaaggc ctgccgccgc tggcggccac gcgcaaggcc atgagccaga tctccggcgc
    10441 catcatcggc gtgacggcgg tgctgatcgc ggtgttcgtg ccgctggcct tcttcaccgg
    10501 ctcggtgggc aacatctacc gccagttctc ggcggtgatg ggggtgtcga tcttcttctc
    10561 ggccttcatg gcgctgtcgc tcacgccggc gctgtgcggc acgctgctca agccggtaca
    10621 ggccggccac cagcaggcca agggcggctt cttcggctgg ttcaaccgcg gctttgcgcg
    10681 caccacgcgc ggctacgaag gcgtggtcgc gcgcctgctg aggcgcagcg gccggctgat
    10741 gctgatctac ctgctgctgg cggccgtggt cggctggatg tacgtgcgcc tgccgaccgc
    10801 cttcctgccg caggaagacc agggcaacct gctggtcaac atccagctgc cgcctggcgc
    10861 caccaccgag cgcacgcaga ccgtggtgga gcagatcgag ggctacctgc tcaagcagcc
    10921 cgaagtgcag agcatggtgg cggtgatggg cttctcgttc tccggccagg gccagaacgc
    10981 cggcatttcc ttcgtcaccc tcaagccctg ggacgagcgc accggcccgc agcacgccgc
    11041 cgatgccgtg gcaggccgcg ccatgggcgc gctgtcgacc atcaaggatg ccttcatctt
    11101 cccgctcaac ccgccgccga tcccggagct gggcgtcagc tcgggcttca gcttccggct
    11161 gcaggaccgc ggtggcaagg gccatgacgc cctgctggcg gcgcgcaacc agatgctggg
    11221 catggccatg cagagcaagc tgctggccgg tgtgcgcccg aacggcctgg aggacgcgcc
    11281 gcagctgcag atcgacatcg accgcgaccg cgcggccgcg cagggcgtga gtttcggcgc
    11341 catcaacagc gtcatctcgg cctcgctggg gtcgagctac gtcaacgact tcccgaacgc
    11401 cggccgcctg cagcgcgtgg tggtgcaggc cgacggaccg gcgcgcatga cgcccgacga
    11461 cgtgctgcgc ctgacggtgc tcaacaacaa gggacaggcg gtgccgctgt cggcgctggc
    11521 cagcacgcgc tgggtcaccg gcgccatgca gaccgtgcgc tacaacggct acccggccat
    11581 gcgcatcgac ggtggcgcgg cgccgggcta cagctcgggc gaggcgatgg ccgaaatgga
    11641 gcgcctggcc agccgcctgc ccgagggctt cggcttcgaa tggaccggcc agtcgcgcga
    11701 ggaaaagctg gccggcaacc aggcgctggt gctgtacggc ttcgccatcc tggcggtggt
    11761 gctggcgctg gcggcgctgt acgagagctg gtcgatcccg ctggcggtga tcctggtggt
    11821 gccgctgggc gtgctgggcg tgctgctggc cgtcaccggg cgcggcctgc tgaacgacgt
    11881 gtacttccag gtcggcctga tcaccatcat cggcctgtcg gccaagaacg ccatcctgat
    11941 catcgagttc gccaaggacc tgcaggcgca gggcaagagc gcgctggagt cggcgctggc
    12001 cgctgcccac ctgcgctttc gccccatcgt catgacctcg ctggcgttcg gcctgggcgt
    12061 gctgccgctg gccatcgcca gcggcgccag ctcggccagt cagcgcgcca tcggcaccgg
    12121 ggtgatgggc ggcattgtgg tgggcaccac gctggcggtg tttttcgtgc cgatcttctt
    12181 cgtcgtcgtg cgcagcctgt tcaagcccaa ccagcgcgag ctggagcgct cgcagcaaca
    12241 cgccgagcac gccggcatca ccgccgcctc ggccgacgcc tacgtcgcgg ccgctgaaca
    12301 gggcatgagc gaagccgaac gccaggcgct gcaccagggc gcgcaaccgc cgctcaccaa
    12361 ggatccatca tgagtcacgc ccgtcatcgc ctctcccgcg ccccgctggt gctggccggc
    12421 ctggtggcgg tcggcctgct ggccggctgc tcgctggtgc cgccctacga acgtccggcg
    12481 gcccccatcg ccagccactg gcccgacacc gccggcgcgc ccggcgccga caccacgacc
    12541 gccgccgacc tgccctggca gcagttcgtg cgcgacgacc agctgcgcga gctgatcggg
    12601 ctggccctga ccagcaaccg cgacctgcgg gtggcggtac agaacatcga acaggcgcgt
    12661 gcccagttcc agatccggcg cgccgaccag ttccccagcg tgggcgccag cgccagcggc
    12721 acgcgctcgg cccccaaccc ctaccaggcg ctgggcgggc ccagcgtgag cagcagctac
    12781 agcgtggggc tgggcgtgtc ggcctgggaa ctcgatttct tcggccgcgt ggccgcgctc
    12841 aaggacgccg cgctggccag ctacctggcc accgaggagg cgcgcaagtc gtcccagatc
    12901 agcctgatcg ccagcgtggc cagcacctgg ctgcaactga agaccgacac cgagctgctg
    12961 gcgctggccg agcgtacgct gggcacgcgc gagcagtcgc tggcgctgac gcggctgcgc
    13021 ttcgagcacg gcgccgcctc ggcgctggat ctgcgccagg ccgagaccct gatcgccaac
    13081 gcccgcgcca cgcgggccca gcagcagcgc ctgcgcgcgc aggacatcca cctgctgacg
    13141 ctgctggtgg gtcagcccct gcccgagcgg ctcatccccg ccgtcccggc cctgcccacg
    13201 ccccagagcc cgagcgagcc gacccaggcc agcgccaccg tggcgccggc ggccgagctg
    13261 cctgcctttg ccgacgtgcc ggccggcctg ccgtccgacg tgctgctgcg ccggcccgac
    13321 atccgcgccg ccgagcagca gctgatcgcc gccaacgccc agatcggtgc cgcacgcgcc
    13381 aatttctttc cgcgcatcac gctgaccggc agcctggggc gcgtcagcag cgacctggat
    13441 ggcctgttcg gctccggcgg cagcagggcc tggtcgttcg gccccgccat cacgctgccg
    13501 atcttcgacg ccgggcgcaa ccaggccggg ctcgatagcg ccctggccgg gcgggacatc
    13561 gccgtggcgc agtacgagaa agccatccag accgccttcc gcgaggtcgc cgatgccctg
    13621 gccggccgcg ccacgctggc cgagcagctg gccgcgctgc aagcgcaggc cgaggccgag
    13681 cgcgcgcgct ttcagctgac cgatctgcgc taccgcaacg gcgtggccag ctcgctcgac
    13741 ctgctggacg cccagcgttc gctgtttgcc atcgaacagg cgctggccca ggcacgactg
    13801 gcccagcgcg ccaacgaggt gcagctctac aaggcgctgg gcggcggctg gagcgagtcg
    13861 ccggccgcag gggcctgagg gcaaggcctg aggggcgtga cacgctgtcc ctataattga
    13921 tttttgtcac gcccgacgca cggcgcgccc gcagcccgcg ccgtgacgcg cacccaacca
    13981 cgagacaacc ccgaccgccc gccagaggaa gccatgagcg acagcacgca cgacgacgcc
    14041 cataccggac ccatcaagtc acccaagcag atgttctggg tcagtgtggc ggccttcgtg
    14101 ctgccggtgt tcatcatcat cggcctggtg tccttcgtca cctcgggcaa caagacctcg
    14161 ccgggcgcgt ccgacatgga acgcgccatc gccgaacgcc tgcagaaggt gggccgagtg
    14221 gaaatccgcg acgccaaccg cccgctggcc agcggcgaag aggtctacaa gacgcaatgc
    14281 gccgcctgcc acgccaccgg cgtctcgggc gcgccgaagt tccagaacgc cggcgactgg
    14341 ggctcgcgcc tgggccaggg ctacgcggcg ctgctgaact cgtcgctcaa aggcaagggc
    14401 gccatggcgc cgcagggtgg cggcaacttc agtgacctgg agatcgggcg cgccgtggtc
    14461 tacatggcca acgctgccgg cggcaaactg gccgagccgg ccgccccggc tgcagccgcc
    14521 agtgcggccg acgccgctcc ggcgcctgcc gcggctgccg cacccgcccc cgccgctgca
    14581 ccggcccccg caccggtggc cgcggcagca ccggcaccgg ccgccgccgc gggcgatgcg
    14641 gccgccggca aggccctgta cgacaaggcc tgcgtcacct gccacgcagc cggtgtggcc
    14701 ggcgcgccca agctgggcga caaggcggcc tgggcgccgc gcattgccga aggcatggac
    14761 gccatgatga agatcgccat caacggcaag ggcgccatgc cgccacgcgg cgcctccacc
    14821 gccagcgacg acgagctgcg cgccgccgtg cagttcatgg tggacgccgc caagtgatcg
    14881 gcccgcaacc ctgaacccgc tagaaaagcc gctgcttcag cggctttctt tttggttcat
    14941 tcagcccaaa agcggcagtt ggacgcgccc gcccgtgccg tgatcggcgt gccaggcaag
    15001 acgcgacgcc gccgcggact cgtgcccgca agggggagca acgccgcatg gcgcggcgag
    15061 cgcggtgcgg gtggatgtgt ggcgctctct cccagccggt gtaggccatc gcagtcgcca
    15121 aaccctgctt ggacgcggcc tgcccgaagt tgacaagcag tcaaccaccg gttccaggct
    15181 caggcctctg gcacccgatc ggcaagcgcc gatagctaca tttttgatag tgccggtcgc
    15241 cctgctcagg gcctgcacga tcgcgcctgc gggctcagta catagccttc gcgctgcggc
    15301 agctcggcgc tgtgcaccgc gacgggctgc cagtcgccgg cttccaccgc gtcggccacg
    15361 gcgcccatgt ccagcgtgtg gacgatgcca aagcccaggt cggtgcgcag aaacacgcgc
    15421 ccctgctcgt ccagccacac cgcctgcacc agtgccggac ggccgtcgtg cgccgacacg
    15481 gcgccgtcgg acgccacacg ccacacccag ggcgccactt ccagctcgac gaatacgcgc
    15541 tgcggcccgt tctggaaata ccagcagccg gccgcgtcgg cggcgtagtt gcgctgaatg
    15601 aagccgatca ggccttcgtg ctgcaagcgc gtgccgcgcg cgccggccac gccgctggca
    15661 aagcccccca gggcctgcgc ctgggcatcg cgcaggtacc agtggccgcg tgcgtccagc
    15721 cccagccagc cgtggcagtg cggcacgttc ggccacttgg ccatggcctg gcgcacgatg
    15781 tcgtccatgc cgctatctta ttgaa
//
LOCUS       DAOOOG010000039        15651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_3980_length_15651_cov_27.966402, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000039 DAOOOG010000000
VERSION     DAOOOG010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 15651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15651
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3980_length_15651_cov_27.966402"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1021
                     /locus_tag="PK242_04855"
     CDS             47..1021
                     /locus_tag="PK242_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ93377.1"
                     /translation="MNFRPIHTLLALTAAATTLLAQPAAAQDFPSKPITLVVPFGPGS
                     GTDQMARLYAKALGDEAKVSVVVDNKGGASGFIAAQHVAKAAPDGYTLMMTTNTTHAA
                     NEHLFKKLPYDPVKDFAPIGLLSTGQMLLLVRPDSPYKSLADLLAAAKKAPGKIDFGS
                     GSSSSQVAGYLLQQMAHVDMLNVPYKSNPQAITDLIGGRFAFMFADAPTALPQVQGGK
                     LRALAASSGKRLASLPDVPTVAEAGVKGYDMSYWFAAYAPAGTPPAVIAKLNQLFAKA
                     SATEEVKASLARTSGVLALGTPEGLAQFQAAESKKWGEVIRAAGIEAQ"
     gene            1018..2268
                     /locus_tag="PK242_04860"
     CDS             1018..2268
                     /locus_tag="PK242_04860"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOZ93378.1"
                     /translation="MTPQAANPTQPAGPLAGVRVLDLTTVFMGPSCTQLLGDLGADVI
                     KIEAPSGDSTRSIGPCGDIGLGPLFLGLNRNKRSVVLDLKQPAAVAALLQLARDADVF
                     ATNVRPAAMRRLGLGPDVLLAANPRLIYASMVGFSQRGPYAAKAAFDDMIQAATGLAA
                     VVGEAAGEVPRYLPITIADRSVGLYAFGVISAALYARERTGRGQSVEIPMFETMIPYV
                     LGDHLYGRTFEPARGDFSYPRLMSPNRRPYKTRDGYVCCLIYTDRHWELFLNAIGKGE
                     LLKTDPRLANIRTRTQAIDALYQLVADELAQRSTAEWRALLPESEIPIFPMHTFESLL
                     HDEHLQQTGFFQHTEHPVVGAVLETAVPSEWSDTVPRHQRPVPALGQHTTEVLREAGF
                     APADIDALLASGAARAAKPPSSTA"
     gene            2289..3068
                     /locus_tag="PK242_04865"
     CDS             2289..3068
                     /locus_tag="PK242_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOZ93379.1"
                     /translation="MTSSPEPELLSRQHDGVLWLTINRPERRNAISPAVLAGLSEALG
                     RANQDREVRAIVLTGVGDKAFCAGADLQTGQSFKFDYSEPTQGMANLFRLTRQLNVPL
                     IARVNGACMAGGMGLMAMCDMAVAAPHAVFGLPEVKVGVFPAQVLSVLSGLIGPRALT
                     ELCITGEPITAAQALQLGLINHVSDDLDAGVQALLGRFLDKSPAAIRRGLYLMKRIGT
                     MSFEQSMAFAESQIALFALTKDAAEGQAAFREKRKPRWSGK"
     gene            3065..4870
                     /locus_tag="PK242_04870"
     CDS             3065..4870
                     /locus_tag="PK242_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HOZ93380.1"
                     /translation="MNTGKIIRIGGASGFWGDSSVGAPQLVQSGQIDYLVFDYLAELT
                     MSILAAARLKKPELGYATDFVSVAMKSVLKDVVAQGIRVVSNAGGVNPEGCADALRAL
                     AAELGVQVKIATVTGDDVMPLLPALREQQPPVAELQSGAPLPARVVSANAYLGALPIK
                     AALDAGAQIVITGRCVDSAVTLGVLMHEFGWQATDYDRLAGASLAGHIIECGCQGTGG
                     LHTDWQAVPDWAHIGYPIVECAQDGSFSVTKPAGTGGLIAPQVVAEQLLYEIHDPARY
                     LLPDVVCDFTQVRLTQTGPERVQVEGARGLPPPASYKVSATYVDGFKTSAQLTIVGFD
                     AVAKARRTGEAILERVGELLQQQGLGPFSATHLEVLGAESPYGPHAQPAAAQMREAIL
                     RLTARHPAKAALELLAREIAPSGTSFAPGTTGAGGRASVSPLIRQYAFLLDKARIAPQ
                     VTLDGAAVSVPNQPPAPVEQAPEAIESIVIQEAYALPAGVESVEVPLLRLAWARSGDK
                     GDASNIGVIARKPEWLPLLRAQLTEARVKDWLAHLVQGPVTRYEVPGIHAFNFVCQQA
                     LGGGGMASLRNDALGKGMGQILLAMPVRVPMSWVN"
     gene            4891..5874
                     /locus_tag="PK242_04875"
     CDS             4891..5874
                     /locus_tag="PK242_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ93381.1"
                     /translation="MPPIHNFSRSRRASLAALAASVAAPAVWAQQDRPIRIIVGFAPG
                     SANDLIARDLARHMAESLKQPVIVENKPGAGGSLGTEAVAKAAPDGLTLGLGTSSQMV
                     MNVALYQNLPFDVERDLRQIGLVGRTPLVLVGKAAGARTLKELIANARANPGQLSYGS
                     GGIGSISHIVGEAFARAADIRLNHVPYKGNGPAMADLAGGHVDMVFDGIITAQPMAQQ
                     GRVRLLAFGGKQRNAAIPDVPTFAEQGLRDYDAYTWNCLFAPAKVADADITRLNQALN
                     AALALPAMKERLARGGADNLGPSTPAQADAFGRKERERWVPFVRSLKLTIN"
     gene            5871..6296
                     /locus_tag="PK242_04880"
     CDS             5871..6296
                     /locus_tag="PK242_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOZ93382.1"
                     /translation="MTPAAPPPEPPPVVIDTNRVLDLWLFGDPEVAPLRQAIEAGRLH
                     WMAQPAMRVELARVLTYPAVARQLLRHRRSADAVLAAFDRWVQRVPAAPPAPVRCRDP
                     DDQIFIDLAVTWRARLLSRDRQIITLARRLTPLGVTVEA"
     gene            6421..6918
                     /gene="ssb"
                     /locus_tag="PK242_04885"
     CDS             6421..6918
                     /gene="ssb"
                     /locus_tag="PK242_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOZ93383.1"
                     /translation="MASVNKVILVGNLGRDPEMRSFPNGDQVANVTLATTDKWKDKQS
                     GEPREHTEWHRLVFNGRLAEIAGQYLRKGSQIYVEGSIRTRKWQDQATGQDRYSTEIR
                     VDQMQMLGSRQGMGGPSDDGGGDGYDAPRRSAPAPRAAPAPRAAAPAARPSSGFDDMD
                     DDIPF"
     gene            6967..7914
                     /locus_tag="PK242_04890"
     CDS             6967..7914
                     /locus_tag="PK242_04890"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HOZ93384.1"
                     /translation="MSCLTSRWPRISMLMLCSLGTGGALAQTQTYATPGNYTYIVPAG
                     VTALQVTVAGGGGGGGGYDLLQTGNGGGGGSGALLQGRLPVTPGSTVQITVGGGGGGG
                     TSDSDGNPAAGGNGGAGLGSGGAGGAPGSTGGSGGGGGGGGSSSLVVGALALQAAGGG
                     GGGGGSGSLGGTAASGASAGGSAPSDANCATGTVGGTGGSPGGGDGGGGGGGGGGVTS
                     GSAGTAGQDGIAHGGPGGAGGSCHLGAVQGLTTSSAGGAGGAGGLASGTAAVTGTHGQ
                     VVITPLLATAVPTLGQWGLALLATVAALLAGRRLRRVAP"
     gene            8138..9157
                     /locus_tag="PK242_04895"
     CDS             8138..9157
                     /locus_tag="PK242_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HOZ93385.1"
                     /translation="MPQRPDALKYLAPNWFAMVMSVAGLAIAWARATPLLGELATAGA
                     LVLAAAAALLFVALLVLSWIRWQHHPEALAADLKHPVRHAFIATLPVSLILLASAAAM
                     LFGPESWVAVLWWLGSLTQFGVTLWVLARWLSADKTQGLSWAALTPVLFIPVVGNVLA
                     PLAGVMLGAPAWTAAQFGLGLLLWPVLLALIGARIATQGLWPERLLAVTFISVAPPSV
                     VGSGVLLLGGPLTLAWMCWGVALFFLLWSGQVLRRMLAQPFSIAFWSLGFPLAAFAAL
                     TLRLAQQDGAAQLPALVALALASLVVLVLGIATWKGWRQGSLLQPEPVAAMAATASAP
                     PPQAQ"
     gene            9218..9805
                     /locus_tag="PK242_04900"
     CDS             9218..9805
                     /locus_tag="PK242_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOZ93386.1"
                     /translation="MQASVTPRHAPAPRLPGDQFIWYVIALELATFCVLFGAFAFTRL
                     WHLELFRTSQQALDVSAGALNTALLLSASWAVARAVHATRADARRAALGWLGLGWLLG
                     AGFVVFKLREYADKAAQGIGMGDGNLFFDFYYILTGFHLLHVLAGLLALALTAVGLAR
                     QRGRLQPHTLETAGAFWHLVDLLWLVLFPLVYVMR"
     gene            9819..10094
                     /locus_tag="PK242_04905"
     CDS             9819..10094
                     /locus_tag="PK242_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HOZ93387.1"
                     /translation="MPPGRPILDLAWLAMLVATAITWVLGRSGWVASAGLAGMALVFA
                     LAWLKGLAVILQFMELQHAPARWRRALIGALTLIVLLILLAYGLSLR"
     gene            complement(10104..11273)
                     /gene="mnmA"
                     /locus_tag="PK242_04910"
     CDS             complement(10104..11273)
                     /gene="mnmA"
                     /locus_tag="PK242_04910"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOZ93388.1"
                     /translation="MQTKQRIVVGLSGGVDSAVAAWLLRQQGHDVVGIFMKNWEDDDD
                     SEYCSSNVDFVDAAAVADVIGIELEHVNFAAEYKDRVFAEFLREYQAGRTPNPDVLCN
                     AEIKFKAFLDHAMRLGAEKIATGHYARVRFNAATGMHELLKGLDPSKDQSYFLHRLNQ
                     AQLARTLFPVGELHKTEVRRIAAEIGLPNAKKKDSTGICFIGERPFREFLGRYLAQQP
                     GPIKDERGRTIGRHQGLSFYTLGQRQGLGIGGVKDRGAARGGGDHAPWFVARKDMARH
                     TLYAVQGHDHPWLLSRALVFDNASWIAGQPPAAGACSAKTRYRQQDAACTLSATAVAG
                     SWRLRFAEPQWAVTPGQSAVLYDGPVCLGGGVIAAAEEYEPDAATGLAQQAPEAM"
     gene            11439..12545
                     /locus_tag="PK242_04915"
     CDS             11439..12545
                     /locus_tag="PK242_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ93389.1"
                     /translation="MHGRLRKLALTTLLCLGGAAQADILIGQTTAVTGPVAASVGETL
                     LGVRAYLDHVNAQGGVHGEKIKLITLDDNFKPPLAAENARKLIEQEGVVALFMSRATP
                     HTQAILPLLAKHKLALVAPSTGAMVFHRPVNPYVFNVRSSYQAEAERAIEHVFQVTLE
                     RVAVVHVDDSFGADCLEGAQKGFERAGKKPVAVIKADREKPDYPAIVKQLTDANAQAV
                     LWIGSSVAVSDGVKALRQAGSAAQVLTVSNNASGGFIKQLGEHARGVVVSQVFPSERA
                     LGIPINREAVALLAGKGELTPQVMEGFASAKVLVEALRRAGPKPTREKIIDALNGLQR
                     FDLGGLELGFSATDHTGLSYVELSIIGRDGRFKR"
     gene            complement(12692..13450)
                     /locus_tag="PK242_04920"
     CDS             complement(12692..13450)
                     /locus_tag="PK242_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOZ93390.1"
                     /translation="MDSFSNLAVLALAAFGAGVLNAIAGGGTFLTFPALVWTGVPPIA
                     ANATSALAVCPGYLGSALGFRAELAALPRALRLREVALSALGGVAGALLLLVTPAKVF
                     AGVVPWLLLFATVLFALGPRLAAWAGGDHGGGLARWRLPALLAVALYGGYFNGGLGIL
                     LMALYLLVGETRMNTVNALKNLNSFVLSVVSVVAFALGGAIVWRQALLMAVFATLGGF
                     AGARLARRLPVAWVRAIVIVTGLAMSALFFARSS"
     gene            complement(13506..14873)
                     /locus_tag="PK242_04925"
     CDS             complement(13506..14873)
                     /locus_tag="PK242_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HOZ93391.1"
                     /translation="MASEAPAAGRPRRDWRASLRIYLSPPSLRMLALGFAAGLPLLLV
                     LGTLSFRLREAGIDRSTIGYLSWVGLAYGFKWAWAPLVDRVPLPGLTRLLGRRRAWLL
                     LAQLGVMAGLVAMAWADPRVSLAAVVWSALAVAFASATQDIALDAYRIESAPTDAQPA
                     LAASYQTGYRLAMIWAGAGVLWLAARAQGDASGYQHEAWRQAYLVMAASMLPGLLTLL
                     RSPEPAPQPLAPARSVGEWLRTALIEPFADFLRRYRWQAALILALIAVYRISDVVMGI
                     MANPFYVDMGYTKDEVAAVTKVYGVAMTLLGAFVGGSLALRFGVMRILMLGALLSALT
                     NLLFAWLAGHGHSLPALIAVVSADNLASGMASAAFVGYLSSLTNLSYSATQYALFSSL
                     MLMLPKFIAGFSGDYVNAFGYAGFFTSTALLGLPVLLLVALAARVPTRHAGQNEPVAQ
                     AGDAP"
     gene            complement(14863..15525)
                     /locus_tag="PK242_04930"
     CDS             complement(14863..15525)
                     /locus_tag="PK242_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ93392.1"
                     /translation="MTATTLSALRYLTVPVTPFAQNCSIVWCERSRQAAIIDPGGDLP
                     RIEAALRELAVTPTQIWLTHAHIDHAGGSTELSARLGLPIVGPHPGDQFWIDGLAEQG
                     RMFGFARAEPFTPTRWLHDGDTVQVGDCELQVRHCPGHTPGHVVFHSPEARRAFVGDV
                     LFAGSIGRTDFPGGNHAQLIASIRERLWPMGDDTVFIPGHGPESTFGEERRHNPFVRD
                     GL"
BASE COUNT         2415 a         5473 c         5455 g         2308 t
ORIGIN      
        1 cgccggcgac cgtcacgcct ttcacccaga acccacagga gacaccatga actttcgacc
       61 catccacacc ctgctcgcgc tgaccgcagc ggccaccacg ctgctggcgc aaccggccgc
      121 agcccaggac tttcccagca aaccgatcac gctggtggtg cccttcggcc ccggcagcgg
      181 caccgaccag atggcacgcc tgtacgccaa ggcgctgggc gacgaagcca aggtgtccgt
      241 ggtggtcgac aacaaaggcg gcgccagcgg tttcatcgcc gcgcagcacg tggccaaggc
      301 ggcgcccgac ggctacacgc tgatgatgac caccaacacc acgcacgcgg ccaacgaaca
      361 cctgttcaag aagctgccgt acgacccggt caaggatttc gcccccatcg gcctgctctc
      421 caccgggcag atgctgttgc tggtcaggcc cgactcgccc tacaagtcgt tggccgatct
      481 gctggccgcc gccaaaaagg cgcccggcaa gatcgacttc ggcagcggct cctcgtccag
      541 ccaggtcgcc ggctatctgc tgcagcagat ggcccacgtg gacatgctga acgtgcctta
      601 caaaagcaac ccgcaggcca tcaccgacct gatcggcggg cgctttgcct tcatgttcgc
      661 cgacgccccc accgccctgc cccaggtgca gggcggcaag ctgcgcgcgc tggcggccag
      721 cagcggcaag cggctggcca gcctgcctga tgtgcccacg gtggccgagg ccggcgtcaa
      781 gggctacgac atgtcgtact ggtttgccgc ctatgcaccg gccggcacgc cgccggccgt
      841 catcgccaag ctcaaccagc tgtttgccaa ggcctcggcc accgaggaag tcaaggccag
      901 cctggcgcgc accagcggcg tgctggcgct cggcaccccg gaaggcctgg cccagttcca
      961 ggccgcggaa tcgaaaaaat ggggcgaggt gatccgcgcg gcgggcatcg aggcgcaatg
     1021 acgccacaag cggcaaaccc cacccagccc gccggcccgc tggctggtgt gcgcgtgctc
     1081 gacctgacca cggtgttcat gggcccatcg tgtacccagt tgctggggga tctgggcgcc
     1141 gacgtgatca agatcgaggc gccgtcgggc gattccacgc gcagcatcgg gccctgcggc
     1201 gatatcggcc tggggccgct gttcctgggc ctgaaccgca acaagcgcag cgtggtgctg
     1261 gacctgaagc agccggccgc ggtggccgcg ctgctgcagc tggcgcgcga tgcggacgtg
     1321 ttcgccacca acgtgcggcc ggcggcgatg cgccgtctgg ggctggggcc cgatgtgctg
     1381 ctggcggcca acccgcgcct gatctacgcc agcatggtgg gcttttcgca gcgcgggccg
     1441 tacgcggcca aggcggcgtt cgacgacatg atccaggccg ccaccgggct ggccgccgtg
     1501 gtcggcgagg ccgccggcga ggtgccgcgc tacctgccga tcaccatcgc cgaccgctcg
     1561 gtggggctgt atgcctttgg cgtcatcagc gcggcgctgt atgcgcgcga gcgcaccggc
     1621 cggggccaga gcgtcgagat cccgatgttc gaaaccatga tcccctacgt gctgggcgac
     1681 cacctgtacg gccgcacctt cgagccggcc cggggcgact tcagctaccc gcgcctgatg
     1741 agccccaacc gccggcccta caagacgcgc gacggctacg tctgctgcct gatctacacc
     1801 gaccggcact gggagctgtt cttgaacgcc atcggcaagg gcgagctgct caagaccgac
     1861 ccgcgcctgg ccaacatccg cacccgcacc caggccatcg atgcgctgta ccagctggtg
     1921 gccgacgagc tggcccagcg cagcacggcc gaatggcgcg cgctgctgcc ggagtcggaa
     1981 attccgatct tccccatgca caccttcgaa tcgctgctgc acgacgaaca cctgcagcag
     2041 accggcttct tccagcacac cgaacacccg gtggtcggcg ccgtgctgga aaccgccgtg
     2101 cccagcgaat ggtccgacac cgtgcctcgc caccagcggc cagtgccggc gctgggccag
     2161 cacaccaccg aggtgctgcg cgaggccggc tttgcgccgg ccgacatcga cgccctgctg
     2221 gccagcggcg cggcgcgcgc ggccaagccc cccagttcca ctgcctgatc gatcggagcg
     2281 atacccccat gacttcttcc cccgagcccg agctgctcag ccgccagcac gacggcgtgc
     2341 tgtggctcac catcaaccgg cccgagcgcc gcaacgccat cagccccgcc gtgctggccg
     2401 gcctgagcga agcgctgggc cgcgccaacc aggatcgcga ggtgcgcgcc atcgtgctca
     2461 ccggcgtggg cgacaaggcc ttttgcgccg gcgccgacct gcagaccggc cagtccttca
     2521 agttcgacta ctccgagccc acgcagggca tggccaacct gtttcggctc acgcgccagc
     2581 tcaacgtgcc gctgatcgcg cgtgtcaatg gcgcctgcat ggccggcggc atgggcctga
     2641 tggcgatgtg cgacatggcg gtggcggcgc cgcacgccgt gtttggcctg cccgaggtca
     2701 aggtgggtgt gtttccggcc caggtgctgt cggtgctcag cggcctgatc gggccgcgcg
     2761 ccctgaccga gctgtgcatc accggcgagc ccatcacagc cgcgcaggcc ctgcagctgg
     2821 gcctgatcaa ccacgtcagt gacgatctgg acgccggcgt gcaggcgctg ctgggccgct
     2881 ttctggataa atcgccggcc gccatccggc gcgggctgta cctgatgaag cgcatcggca
     2941 ccatgtcgtt cgagcagtcg atggccttcg ccgaaagcca gatcgccctg ttcgccctga
     3001 ccaaggacgc ggccgaggga caggccgcct ttcgcgaaaa acgcaaaccc cgttggagcg
     3061 gcaaatgaac accggaaaaa tcattcgcat cggcggcgcc agcggcttct ggggcgactc
     3121 cagcgtgggc gcgccgcagc tggtgcaaag cggccagatc gactatctgg tgttcgacta
     3181 cctggccgag ctgaccatgt cgatcctggc cgccgcgcgc ctgaagaagc ccgagctggg
     3241 ctacgccacc gacttcgtca gcgtggccat gaagagcgtg ctgaaggacg tggtggccca
     3301 gggcatccgc gtggtcagca acgccggcgg cgtcaacccc gagggctgcg ccgacgcgct
     3361 gcgcgccttg gccgccgagc tgggcgtgca ggtgaagatt gccaccgtga cgggtgacga
     3421 cgtcatgccc ctgctgcccg cgctgcgcga gcagcagccg ccggtggccg agctgcaaag
     3481 cggcgcgccg ctgccggcca gggtggtgag cgccaacgcc tacctcggcg cgctgccgat
     3541 caaggcggcg ctggacgccg gcgcgcagat cgtcatcacc ggccgctgcg tcgattcggc
     3601 ggtgacgctg ggcgtgctga tgcacgagtt cggctggcag gccacggact acgaccggct
     3661 ggccggcgcc agcctggcgg gccacatcat cgaatgcggc tgccagggca cgggcgggct
     3721 gcacaccgac tggcaggccg tgcccgactg ggcgcacatc ggctacccca tcgtcgagtg
     3781 cgcgcaagac ggcagcttca gcgtcaccaa acccgccggc accggcggcc tgattgcgcc
     3841 gcaggtggtg gccgagcagc tgctgtacga aatccacgac ccggcccgct acctgctgcc
     3901 cgacgtggtg tgcgacttca cccaggtgcg gctgacccag accggccccg agcgggtaca
     3961 ggttgaaggt gcgcgcggcc tgccgccgcc agccagctac aaggtcagcg ccacctatgt
     4021 ggacggcttc aagacctcgg cgcagctgac catcgtcggc ttcgacgccg tggccaaggc
     4081 gcgccgcacc ggcgaggcca tcctcgagcg cgtgggcgag ctgctgcagc agcagggcct
     4141 gggccccttc agcgccaccc acctggaggt gctgggcgcg gaaagcccct atggcccgca
     4201 cgcccagccg gcggcggcgc agatgcgcga agccatcctg cgcctgaccg cgcgccaccc
     4261 cgccaaggcg gcgctggagc tgctggcgcg cgagatcgcg ccgtcgggca cctcgtttgc
     4321 gcccggcacc accggcgccg gcgggcgcgc cagcgtgtcg ccgctgatcc ggcagtacgc
     4381 cttcctgctc gacaaggcgc gcatcgcgcc ccaggtgacg ctcgacgggg ccgcggtttc
     4441 agtgcccaat cagcctccag cgcccgtgga acaagcgcca gaagctatcg aatcaatagt
     4501 tattcaggag gcctacgcac tgcctgccgg cgtagaaagc gtcgaggtgc cgctgctgcg
     4561 cctggcctgg gcgcgctctg gcgacaaggg cgacgcctcc aacatcggcg tcatcgcccg
     4621 caagcccgaa tggctgccgc tgctgcgcgc gcagctgacc gaagcgcggg tcaaggactg
     4681 gctggcgcac ctggtgcagg ggccggtgac gcgctatgaa gtgcccggca tccacgcctt
     4741 caacttcgtc tgccagcagg cgctgggcgg cggcggcatg gcctcgctgc gcaacgacgc
     4801 gctgggcaag ggcatggggc agatcctgct ggccatgccg gtgcgcgtgc ccatgagctg
     4861 ggtgaactga acacaagcaa ggagacaagc atgcccccga tccacaactt ttcccgctcg
     4921 cgccgcgcca gcctggcggc gctggccgcc agcgtcgccg ccccggcggt ctgggcccag
     4981 caggaccgcc cgatccgcat catcgtcggc tttgcgcccg gcagcgccaa cgatctgatc
     5041 gcgcgcgatc tggcgcgcca catggccgag tcgctgaagc agccagtgat cgtcgagaac
     5101 aagccgggcg ctggcggttc gctgggcacc gaggcggtgg ccaaggcggc gcccgacggc
     5161 ctgaccctcg gcctgggcac cagctcacag atggtgatga atgtggcgct ctatcagaac
     5221 ctgccgttcg atgtcgagcg cgatctgcgc cagatcggcc tggtcggccg cacgcccctg
     5281 gtgctggtcg gcaaggccgc gggcgccagg acgctcaagg agctgatcgc caacgcgcgc
     5341 gccaacccgg gccagctcag ctacggctcg ggcggcatcg gatcgatcag ccacatcgtg
     5401 ggcgaagcct ttgcgcgcgc cgccgacatc cgcctcaacc acgtgcccta caagggcaac
     5461 ggaccggcca tggccgatct ggccggcggc catgtggaca tggtgttcga cggcatcatc
     5521 accgcccagc ccatggccca gcagggccgc gtgcgcctgc tggcctttgg cggcaagcag
     5581 cgcaacgcgg ccatccccga cgtgcccacc tttgccgaac agggcctgcg cgactacgac
     5641 gcctacacct ggaactgcct gttcgccccg gccaaggtgg ccgacgccga catcacgcgc
     5701 ctgaaccagg cgctcaacgc cgccctggct ctgccggcga tgaaggagcg gctggcgcgc
     5761 ggcggcgcgg acaacctggg cccctccacc ccggcccagg ccgacgcctt cggtcgcaag
     5821 gaacgcgagc gctgggtgcc cttcgtgcgc agcctcaagc tgaccatcaa ttgaccccgg
     5881 ccgcgccacc gcccgaaccg ccaccggtgg tgatcgacac caaccgggtg ctcgatctgt
     5941 ggctgttcgg ggatcccgag gtggcgcccc tgcgtcaggc catcgaagcg ggccggctgc
     6001 actggatggc gcagccggcc atgcgggtcg aactggcccg ggtgctgacc taccccgccg
     6061 tggcacggca gctgctgcgc caccggcgca gcgccgacgc cgtgctggcg gcgttcgacc
     6121 gctgggtgca acgggtgcct gcggcgccgc ccgcaccggt gcgctgccgc gaccccgacg
     6181 accagatctt catcgacctg gccgtgacct ggcgcgcccg gctgctcagc agggaccggc
     6241 agatcatcac cctggcgcgc cggctgacgc cgctgggtgt gaccgtcgag gcgtgaccgc
     6301 cgggttctgg ccaggcatgc agcgcccgag cgcgccgcgc cggcccggcg gcggtgcatg
     6361 gggcacaatc acgggctcga tcacccgctg tttcccattc cacttcacag gaacaacgcc
     6421 atggcctccg tcaacaaagt catcctcgtc ggcaacctgg ggcgcgaccc cgaaatgcgc
     6481 tctttcccca acggcgatca ggtggccaac gtgacgctgg ccaccaccga caagtggaag
     6541 gacaagcagt ccggcgagcc gcgcgaacac accgaatggc accgcctggt gttcaacggc
     6601 cggctggccg aaatcgccgg tcagtacctg cgcaagggca gccagatcta cgtcgaaggc
     6661 agcatccgca cccgcaagtg gcaggaccag gccaccggcc aggaccgcta cagcaccgaa
     6721 attcgcgtcg accagatgca gatgctgggc agccgtcagg gcatgggcgg cccgtcggac
     6781 gacggtggtg gcgacggcta cgacgccccg cgccgcagcg caccggcacc gcgcgccgcc
     6841 cccgccccgc gcgcggcggc ccctgccgcg cggccgtcgt cgggcttcga tgacatggac
     6901 gacgacattc ccttttgaaa ccactggggt ctcccaaccc ctcactactg cggagaggag
     6961 aagaagatga gttgtctcac gtcgcgctgg ccgcgcattt cgatgctgat gttgtgcagt
     7021 cttggaacgg gcggtgcgct ggcccagaca cagacctacg ccacacccgg caactacacc
     7081 tacatcgtgc cggcgggcgt cactgccttg caggtcacgg tggccggtgg cggtggtggt
     7141 ggtggtggct acgacctctt gcaaaccggg aacggcggtg gcggcggctc tggtgcattg
     7201 ctgcagggca ggctccctgt gacgcccggc agcacggtgc agatcaccgt gggtggtgga
     7261 ggtggcggcg gcacttccga ctctgatggc aatccggccg ctggtggcaa cgggggtgcg
     7321 ggattgggtt caggaggcgc cggcggcgcg ccgggcagca ccgggggcag tggtggaggc
     7381 ggtggcgggg gtggcagcag cagcctggta gtgggcgctc tcgcgcttca ggccgcgggg
     7441 ggtggcggtg gcggcggcgg cagcggatcg ctcggtggca ctgctgcctc aggggcgtca
     7501 gctggtggct cggcgcccag cgatgccaac tgcgccacag gaacggttgg cggcaccggt
     7561 ggcagccccg gtgggggaga tggtggtggt ggcggcggtg gtggcggcgg cgtcacttcc
     7621 ggaagcgcag gcacagccgg gcaggacggc atcgcccatg gcggccctgg tggtgcaggc
     7681 ggttcgtgcc atctcggagc ggtgcaaggc ctgaccacca gcagtgccgg tggcgcaggt
     7741 ggtgccggcg gcttggcatc tggtactgcc gcagtcacgg ggacccatgg acaggtcgtc
     7801 atcaccccgc tgctggcgac ggccgtgcct acgctcggtc aatgggggct cgccctgctg
     7861 gccacagtgg ccgcgctgct ggcgggtcgg cgtctgcggc gcgtggcgcc atgaacgggc
     7921 tcagtgatgc aacccgatgg ggcgcatcac ttggctgcaa ctgagcgaca gaccacaggt
     7981 tcatgcgttg ttgagggttg gaccatgttg cgtgtggaat caggtcggtt tgcagccgcc
     8041 cggcaggcag acccgatgac gccctgacgc acccatgcaa tgcccgcacc gctgagcacc
     8101 agcgccctgc cggtgcgggc cgtggcatca tcgccgcatg ccacagcgac ccgacgcctt
     8161 gaagtacctc gcccccaact ggtttgccat ggtgatgagc gtggccggcc tggccatcgc
     8221 ctgggcgcgc gccacgccgc tgctgggtga gctggccacc gccggcgcgc tggtgctggc
     8281 ggcggcggcg gcgctgctgt tcgtggcgct gctggtgctg tcgtggatcc gctggcagca
     8341 ccaccccgag gcgctggcgg ccgacctgaa gcacccggtg cgccacgcct tcatcgccac
     8401 gctgccggtg tcgctgatcc tgctggcgtc ggcggcggcc atgctgttcg gtcccgagag
     8461 ctgggtcgcg gtgctgtggt ggctcggctc gctgacgcag ttcggcgtca cgctgtgggt
     8521 gctggcgcgc tggctcagcg ccgacaagac ccagggcctg agctgggcgg cgctgacgcc
     8581 ggtgctgttc atcccggtgg tgggcaacgt gctggcgccg ctggccggcg tgatgctggg
     8641 cgcgccagcc tggacggcgg cgcagttcgg cctcgggctg ctgctgtggc cggtgctgct
     8701 ggcgctgatc ggcgcgcgca tcgccaccca gggcttgtgg ccggagcggc tgctggccgt
     8761 caccttcatc agcgtggcgc cgccgtcggt ggtcggcagc ggcgtgctgc tgctgggcgg
     8821 tccgctgacg ctggcctgga tgtgctgggg ggtggcgctg ttctttctgc tgtggagcgg
     8881 tcaggtgctg cggcgcatgc tggcgcagcc gttttccatc gccttctggt cgctcggctt
     8941 tccgctggcg gcctttgccg cgctgacgct gcggctggcg cagcaggacg gcgcggccca
     9001 gctgccggcc ctggtggcgc tggcgctggc gtcgctggtg gtgttggtgc tcggcatcgc
     9061 cacctggaag ggctggcgcc agggcagcct gctgcaaccc gagccggtgg ccgcgatggc
     9121 cgcgaccgcc agcgcgccgc caccgcaggc gcagtgaacc gttggatgaa gattcaggct
     9181 tctgacgccc gtcggacgtg cgtcagcagc tatcattttg caagcatctg tgaccccacg
     9241 ccacgccccc gcgccacgcc tgccgggtga ccagttcatc tggtacgtga tcgcgctgga
     9301 gctggccacc ttctgcgtgc tgttcggcgc ctttgccttc acgcggctgt ggcacctgga
     9361 gctgtttcgc acctcgcagc aggcgctgga cgtcagcgcg ggcgccctca acaccgcgct
     9421 gctgctgtcg gccagctggg cggtggcgcg ggcggtgcac gccacgcgcg ccgatgcgcg
     9481 ccgcgcggcg ctgggctggc tgggcctggg ttggctgctg ggcgcaggct tcgtggtctt
     9541 caagctgcgg gaatacgccg acaaggcggc gcagggcatc ggcatgggcg acggcaacct
     9601 gttcttcgac ttctactaca tcctgaccgg ctttcacctg ctgcacgtgc tggccggcct
     9661 gctggcgctg gcgctgaccg ccgtcggtct ggcgcgccag cgcggccgac tgcagccgca
     9721 cacgctggag accgccggcg ccttctggca cctggtggac ctgctgtggc tggtgctgtt
     9781 cccgctggtc tatgtgatgc gctgaggcgc gaagcgacat gcccccagga cgcccgatcc
     9841 tcgaccttgc ctggctggcc atgctggtgg ccaccgccat cacctgggtg ctgggccgca
     9901 gcggctgggt ggcgtcggcg gggctggccg gcatggcgct ggtgtttgcc ctggcctggc
     9961 tcaagggcct ggcggtgatc ctgcagttca tggagctgca gcacgccccg gcgcgctggc
    10021 gacgggcgct gatcggtgcg ctgacgctga tcgtgctgct gattctgctg gcctacgggc
    10081 tgtcgctgcg ttaatgctat tgtttacata gcttctggcg cttgctgcgc aagccctgtg
    10141 gccgcatcgg gttcatattc ttcggccgcc gcgatgaccc cgccgcccag acacaccggg
    10201 ccgtcgtaca gcaccgcgct ctggccggga gtgaccgccc attgcggctc ggcgaagcgc
    10261 aggcgccagc tgcccgccac cgcagtggcc gacagggtgc aggcggcgtc ctgctggcgg
    10321 tagcgggtct tggccgagca ggcgccggcg gcgggtggct ggccggcgat ccagctggcg
    10381 ttgtcgaaca ccagcgcgcg cgacagcagc cacgggtggt cgtggccttg cacggcgtac
    10441 agggtgtgtc gcgccatgtc cttgcgcgcc acgaaccagg gcgcgtggtc gccgccgccg
    10501 cgcgccgcgc ctcggtcctt gacaccgccg atgcccagcc cctggcgctg gccgagggtg
    10561 tagaacgaca ggccctggtg gcgaccgatg gtgcgcccgc gctcgtcctt gatcggcccc
    10621 ggctgctggg ccaggtagcg cccgagaaat tcgcggaacg gccgctcgcc gatgaagcag
    10681 atgccggtgg agtccttctt cttcgcgttg ggcaggccga tttcggcggc gatgcggcgc
    10741 acctcggtct tgtgcaactc gccgaccggg aacagggtgc gcgccagttg cgcctggttc
    10801 agccggtgca ggaaatagct ctgatccttc gacggatcca gccccttgag cagctcgtgc
    10861 atgccggtgg cggcattgaa gcggacgcgg gcgtaatgcc cggtggcgat cttttcggcg
    10921 cccaggcgca tggcgtggtc gaggaaggcc ttgaacttga tctcggcgtt gcacagcacg
    10981 tcggggttgg gcgtgcgccc ggcctggtat tcgcgcagga actcggcgaa cacgcggtcc
    11041 ttgtattcgg cggcgaagtt gacgtgttcg agctcgatgc cgatcacatc ggccaccgcc
    11101 gccgcgtcca cgaagtcgac gttggacgag cagtactcgc tgtcgtcgtc gtcttcccag
    11161 ttcttcatga agatgccgac cacgtcgtgg ccctgctggc gcagcagcca ggcggcgacg
    11221 gccgaatcca ccccgccgga cagcccgacc acgatgcgtt gcttcgtctg catctgacca
    11281 ttttcgcagg cactgccgtc cattttgggt tagaccgggt ggacagcgtg aagggattgt
    11341 gcgtatggtt gcgttcccct attccagttg catggctgcc tatgttcact gcgctcaaac
    11401 gtgaccccat cctgatgtcc cgtggattcg ctccgggcat gcatggccgt ctgcggaagt
    11461 tggcgctgac gacattgctg tgcctgggcg gcgcggcaca ggccgacatc ctgatcggcc
    11521 agaccaccgc cgtgaccggc cccgtggccg ccagcgtggg cgagacgctg ctgggtgtgc
    11581 gcgcctacct ggaccacgtc aacgcccagg gtggtgtgca tggcgagaag atcaagctca
    11641 tcacgctcga cgacaacttc aagccgccgc tggcggcgga aaacgcgcgc aagctgatcg
    11701 agcaggaggg cgtggtggca ctgttcatga gccgggccac gccgcacacc caggccatcc
    11761 tgccactgct ggccaagcac aagctggcgc tggtggcgcc ttccaccggc gccatggtgt
    11821 ttcaccggcc tgtgaatccg tatgtgttca acgtgcgctc cagctaccag gcggaggccg
    11881 agcgtgccat cgagcacgtc ttccaggtca cgctggagcg ggtggccgtg gtgcacgtgg
    11941 acgacagctt cggcgccgac tgcctggaag gcgcgcagaa gggctttgaa cgtgccggca
    12001 aaaagccggt ggccgtcatc aaggccgacc gcgagaagcc cgactacccg gccatcgtca
    12061 aacaactgac ggacgcgaac gcccaggcgg tgctctggat cggctccagc gtggcagtga
    12121 gcgatggcgt caaggcgctg cgccaggcag gatcggcggc acaggtgctg acggtttcca
    12181 acaacgcctc gggcggcttc atcaagcagc tcggcgagca tgcgcgtggg gttgtcgtgt
    12241 cgcaggtgtt ccccagcgag cgagcgctgg gcatcccgat caaccgcgaa gccgttgccc
    12301 tgctggccgg caagggggag ctgacgccgc aggtgatgga gggttttgcc agtgccaagg
    12361 tgttggttga agcgctgcgc cgcgccggcc ccaagcccac gcgcgagaag atcatcgacg
    12421 cgctcaacgg cctgcagcgc ttcgacctgg gcgggctgga gctgggcttc agcgccaccg
    12481 accacacggg gctgagctac gtggaactgt ccatcatcgg ccgcgatggt cgcttcaaac
    12541 gatagacggc ccacacccag actccactcg cttcgcgacg tttcgccgtc tcggaggaac
    12601 gcagccgctg gccggatcag tctgggccac ggctggcgct ggcatggcct gtcccacgta
    12661 ctgcgggctc tcggaccgtg atgcgccgcc ttcaggaact gcgcgcgaaa aacagcgcgc
    12721 tcatcgcgag accggtgacg atcacgatgg cacgcaccca ggccaccggc agccgccgcg
    12781 ccagccgcgc gccggcaaaa ccacccaggg tggcgaacac cgccatcagc agcgcctggc
    12841 gccacacgat ggcgccgccc agcgcgaagg caaccaccga caccaccgac agcacgaagg
    12901 agttgaggtt tttcagcgcg ttgacggtgt tcatgcgggt ttcgcccacc aacaggtaca
    12961 gcgccatcag caggatgccc agcccgccgt tgaaatagcc gccgtacagc gccaccgcca
    13021 gcagcgccgg caggcgccag cgcgccagac cgccgccatg gtcgccgcca gcccaggcgg
    13081 ccagccgcgg cccaagtgcg aacagcacgg tggcgaacag cagcagccag ggcaccacgc
    13141 cggcgaacac cttggcgggg gtgaccagca gcagcagtgc gccggccacg ccgcccagcg
    13201 cgctcagcgc cacctcgcgc agccgcagcg cacgcggcag cgccgccagc tcggcccgaa
    13261 agcccagcgc actgcccagg tagccgggac acacggccag cgcgctggtg gcgttggcgg
    13321 cgatcggcgg cacgccggtc cacaccagcg ccgggaaggt caggaaggtg ccgccgccgg
    13381 cgatggcgtt gagcacaccg gcgccaaagg cagccagcgc cagcacggcc aggttgctga
    13441 aggagtccat gtcgatgcgc gccgccacac cggcacggcg ccccagatac tacaaaatca
    13501 atagcttatg gcgcatcacc cgcctgcgcc acaggctcat tttgccctgc atgacgggtc
    13561 ggcacccgcg ccgccagcgc caccagcagc agcaccggca gacccagcag tgcggtgctg
    13621 gtgaaaaagc cggcgtagcc gaaggcgttg acgtagtcgc ccgaaaatcc ggcgatgaac
    13681 ttgggcagca tcagcatcag cgaggaaaac agcgcgtact gcgtggccga gtagctgagg
    13741 ttggtcaggc tcgacaggta accgacgaac gccgccgagg ccatgcccga ggccaggttg
    13801 tcggccgaca ccacggcgat cagcgccggc aggctgtgcc catggccggc cagccaggcg
    13861 aacagcaggt tggtcagcgc cgacagcagc gcccccagca tcaggatgcg catgactccg
    13921 aagcgcagcg ccagcgaacc gccgacaaag gcccccagca gggtcatcgc cacgccgtag
    13981 accttggtca ccgccgccac ttcgtccttg gtgtagccca tgtcgacgta gaacgggttg
    14041 gccatgatgc ccatcacgac gtcgctgatg cggtagacgg cgatcagcgc caggatcagg
    14101 gccgcctgcc agcggtagcg gcgcaggaag tcggcgaacg gctcgatcag cgcggtgcgc
    14161 agccactcgc ccacgctgcg cgccggcgcc agcggttgcg gcgccggctc gggcgagcgc
    14221 agcagcgtca gcagccccgg cagcatggac gcggccatca ccaggtaggc ctgccgccag
    14281 gcctcgtgct ggtagccgct ggcgtcgccc tgcgcgcgcg ccgccagcca cagcacgccg
    14341 gcgccggccc agatcatggc cagccggtag ccggtctggt agctggccgc cagcgccggc
    14401 tgcgcatcgg tgggcgccga ttcgatgcgg taggcgtcca gcgcgatgtc ctgcgtggcc
    14461 gaggcgaagg ccacggccag cgcgctccac acgacggcgg ccagcgacac gcgcggatcg
    14521 gcccaggcca tggccaccag cccggccatc acgccgagct gggccagcag cagccaagcg
    14581 cgccggcgcc ccagcaggcg cgtcagcccg ggcagcggca cgcggtccac caggggcgcc
    14641 caggcccact tgaagccata ggccagcccc acccagctga ggtagccgat ggtggagcgg
    14701 tcgatgccgg cctcgcgcag gcgaaagctc agcgtgccca gcaccagcag cagcggcagt
    14761 ccggcggcaa agcccagcgc cagcatgcgc aggctgggcg gcgacaggta gatgcgcagg
    14821 ctggcgcgcc agtcgcgccg cggccgccct gccgccggcg cctcagaggc catcgcgcac
    14881 gaacgggttg tggcgccgtt cctcgccaaa cgtgctctcc ggcccgtggc cggggatgaa
    14941 cacggtgtcg tcgcccatgg gccacaggcg ctcgcggatg ctggcgatca gctgggcgtg
    15001 gttgccgccc ggaaagtcgg tgcgcccgat gctgccggca aacagtacgt cgccgacgaa
    15061 ggcgcggcgc gcctcgggcg aatggaacac cacgtgcccc ggggtgtggc cagggcagtg
    15121 gcgcacctgc agctcgcagt cgccgacctg caccgtgtcg ccatcgtgca gccagcgcgt
    15181 gggcgtgaac ggctcggcgc gcgcaaagcc gaacatgcgg ccctgctcgg ccagaccgtc
    15241 gatccagaac tggtcgcccg ggtgcggacc gacgatcggc aggcccagcc tggcgctcag
    15301 ttcggtgctg cctccggcgt ggtcgatgtg ggcgtgggtg agccagatct gggtcggggt
    15361 caccgccagc tcgcgcagcg cggcttcgat gcgcggcaga tccccacccg gatcgatgat
    15421 cgccgcctgc ctgctgcgct cgcaccagac gatggagcag ttctgcgcaa acggcgtcac
    15481 cggtacggtc aggtaacgca gcgcggaaag ggtggtggcg gtcatggtgc ggtacacaac
    15541 agcggggccg gaaagcaaaa atcccgcacg gagcgggatt tttgaccggg gtatcctggt
    15601 tgcgcgagga ggatttgaac ctccgacctt tgggttatga gcccaacgag c
//
LOCUS       DAOOOG010000040        15321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4124_length_15321_cov_26.597340, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000040 DAOOOG010000000
VERSION     DAOOOG010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 15321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15321
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4124_length_15321_cov_26.597340"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PK242_04935"
     CDS             complement(<1..214)
                     /locus_tag="PK242_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOZ93393.1"
                     /translation="MLPIENPSTHDTTRIDDTRIRAVRPLLTPALLEEWLPAPDAAQD
                     LVEASRTALSRVLHGQDDRLVVVVGPC"
     gene            complement(272..544)
                     /locus_tag="PK242_04940"
     CDS             complement(272..544)
                     /locus_tag="PK242_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOZ93394.1"
                     /translation="MTAAPRRAALPQIDPTRCTGCGWCVAVCAPHVLSLHADGGRKAS
                     ALDDAAGCTGCALCALRCPFGAIRMRRRPAQAPEAGPASDAGRISN"
     gene            complement(555..1139)
                     /locus_tag="PK242_04945"
     CDS             complement(555..1139)
                     /locus_tag="PK242_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HOZ93395.1"
                     /translation="MWLLVLGLLLFLGVHSVRIVADGWRTRGIARLGAGRWKGLYALL
                     SLLGLALIVWGWALARRQPVVWWVPPAGLRHLNALFTLAAFVLLAAAYVPGNLVKARL
                     RHPMVLGVALWALGHLLASGSGAGLLLFGAFLLWALLDWRAAMRRDRAQGTAAPPGRW
                     GATLLTLLVGALAWVAFAFWLHAAWIGVAPLGAL"
     gene            1265..2215
                     /gene="rocF"
                     /locus_tag="PK242_04950"
     CDS             1265..2215
                     /gene="rocF"
                     /locus_tag="PK242_04950"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HOZ93396.1"
                     /translation="MTAPAPPPAITLIGAPTDVGGGARGASMGPEALRVAGLVEALRA
                     LGADVQDAGNLAGPPNPGLPPVQGFRHLAEVTAWNQAVHQAVQASLQDGRLPLLLGGD
                     HSLAIGSISAVARHCRARGQPLRVLWFDAHADFNTAALTPSGNLHGMPVASLFGDGPP
                     ALVGLGGFSAAQPALRAHELRQIGIRSVDAGEKRLVHEAGIEVFDMRAIDELGMRAVM
                     ARCLAGLDAGSHLHVSLDVDFLDPDIAPGVATTVRGGPSYREAQLCMEMVADTGRLGS
                     LDIVELNPACDSHNRTAELAVDLVESLFGKSTLMRQRGRG"
     gene            complement(2379..4439)
                     /locus_tag="PK242_04955"
     CDS             complement(2379..4439)
                     /locus_tag="PK242_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOZ93397.1"
                     /translation="MPDAARHLRARLMRALQVGEWAYDPATQRFWLSGDAAALLGVVP
                     GSITVEHLWPRLAEESAQRLARALLSCQQGGAPIDVEVQLAPGEEPRRWLRVVGEADG
                     AATAAGTAIQGAVQDVSSRKQAQDETLRLAMRLTTTLASITEAFVTLDRAGRFMYVNE
                     ESERLLGASSRELLDEPIWQHLQGQQPGLLQARVHEALRQDQRVEFEDFYPAAGKWIE
                     LRAYPYAEGLAVYLRDVSERKAAEAKIHHLAFYDPLTGLPNRQLLIERLEAARAACAG
                     QGPTGALMFIDLDHFKVLNDTRGHHTGDLLLRGVAARLRGGVREGDTVARLGGDEFVV
                     LLPALDRDPAQAARQAQAVARKVLARLIEPFDLDGHRHHCTTSIGVTLFGAGPDSVAE
                     LLQQADLAMYQAKHMGRNTVAFFDPAMQAALNASTTLVADLRAALGAERQLELHFQPQ
                     FDRARRITGVEALLRWQHPQRGLVGPTEFIPVAEDSGLIVPLGLWALTQACRQLARWQ
                     AQPHTAALSVSVNVSAQQFRHPDFVDQVTAVIRQSGAPAARLRLELTESLLADRLDIT
                     LAQMDRLRQLGVALSLDDFGTGYSSLAYLKRLPLDQLKIDRAFVADVLTDSGDAAIAS
                     AIIAMAHSLGLKVVAEGVETEAQQRLLSQRGCDGFQGFLLAPPMPAPQVEHFIARHTT
                     AGAA"
     gene            4762..5067
                     /locus_tag="PK242_04960"
     CDS             4762..5067
                     /locus_tag="PK242_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOZ93398.1"
                     /translation="MNVFQEFRRCRDLGTLRSRVTELSARFGALSRLDILTAVHAGRQ
                     QAICFLRMQTAEQERTLMKTLGVGRFGGEIVFVVDLPEPTGFDDSLPSSQWADSEVQ"
     gene            5067..6248
                     /gene="wecB"
                     /locus_tag="PK242_04965"
     CDS             5067..6248
                     /gene="wecB"
                     /locus_tag="PK242_04965"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOZ93399.1"
                     /translation="MDARVRDRNRGGVAAAASILIVCGTRPEIIKLAPLYHELRRQPW
                     AHVVWLHTGQHAQMAQPILDSFDIRPDLTLTRQGDSLLDFSLGCRAQLEAVLAGGRWA
                     AVVVQGDTESAFQGALAGFYHGVPVAHVEAGLRTYNLNRPFPEEGLRQMIGRIARLHF
                     APTARSRQALLSEGVPQQRIWVTGNTVIDAQQWVCAHRGIGRRVNGRGHLLVTMHRRE
                     NWGQDIERVCRAIARIAREQPDLEVLFPVHLNPVVHGPVHQLLAGLPNVRLTAPLDYL
                     EMQQALRDAWIVLTDSGGLQEEAPTFGIPLLVLREETERPEALEAGCAAIVGTATDTI
                     VEQVRRLWREPAEFLRMQRAGNPFGDGTASRQIARHLARELGVHASHAGARQEQAHVV
                     G"
     gene            6235..8451
                     /locus_tag="PK242_04970"
     CDS             6235..8451
                     /locus_tag="PK242_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HOZ93400.1"
                     /translation="MWWVDYVSSYLYGLKSVALALAVIILVSSLDDLFIDLAYWCRRL
                     WRSATIYRRHARARPADLQARDEKPMAIMVPAWQEHGVIDRMAELAATTLDYENYHIF
                     VGTYPNDPQTQSDVEKVCARFPNVHKVVGARDGPTSKADCLNNVLDAIFQFERRAGIH
                     FEGFILHDSEDVLSPMELRVFNHLVGRKDLIQLPVYPLPRPWTDFTSCHYLDEFAESH
                     GKDLLVREALVGQVPSAGVGTCFSRKAVMALLSHGKGIAFDVKSLTEDYDIGLRLKGM
                     GMQEVFVRFPMKPHHARDAGAPLGQNAREASTVCVREYFPSVTRAAIRQKSRWIIGII
                     YQGTKTMGWSRHWGLNYFLWRDRKGVITNFAGMLATLVLLQLVALWLYQRLVTDSYQF
                     LSIFEDSAWLTLLLVANLVLLCNRAVQRIYFVSSYYGIRQGLLSLPRMAWGNVINFFA
                     NWRALMQTLQAQDVRRIAWAKTEHDFPTLGDEGGAREPLGQILVAQSALTQEQLDEAL
                     AEPVPGLRLGAMLVHRGLVTPEQLAQAVATQSRADWEPFDSRQLGPELVACVPAAVAL
                     HYAVLPMRQTEDTLVLACESYLDPVSLAAIARKLDRKVSYVIVPKGEVTVGLRRWYGR
                     DGDLVAADAGRGGVHATPQTAALMWRVYVGGQLLLGDILCALGHVDPAALKSVLIQRA
                     KTGQRLGEFMVARGLLTPQVLDEALALQRSLQPSIDTLLGGLVPGRVGSGPLATEA"
     gene            8452..>9033
                     /locus_tag="PK242_04975"
     CDS             8452..>9033
                     /locus_tag="PK242_04975"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ93401.1"
                     /translation="MKRLSPSLALTLLLAALPALGQPVDLGPGVSGYTRFLVYPHLQK
                     GFQALREAQQTLAYEEFEQALKLAPDSAVIAGYLVEAYRHFGDHDKARQLLQRQLARH
                     PDDPRLRQALASLGPAAPTRPPAPQVTAGGAGDRAAELAAAPAPAPAPAPAVEAAPPR
                     ARAAATPDAASADARQTSRRDRASSAGGRPPSRS"
     assembly_gap    9034..9133
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9231..11480
                     /locus_tag="PK242_04980"
     CDS             9231..11480
                     /locus_tag="PK242_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage N4 adsorption protein A"
                     /protein_id="HOZ93402.1"
                     /translation="MPPANQAPQHGARAAYALADAAYKASATGDFAAAARDARQALQL
                     QPDNADYRRLLAYALLETGAYADVEALAGERSSSDPALLALADQARRQRAYVEFEAAS
                     RSLAAGDVDGAVTQAARGAQLAPATLAQQVQWLGALAAAQRWSALDDAASQVLQRPGM
                     DDADIRLLRAHARQRLGRLDDANADYDRALALSSDPDTRSRNARLIVADAALAAGQTV
                     RAEQLLAALSGPADAAVDARLRQASAGARRTLLPSPTVVPALATPRVVCVGSAHTPYC
                     ELWPGDAPADPGAVHADAALRAYAARSYAVAATQARSAVEQSPANRRYQLLLVRALLA
                     ADQPQQALERANEFLSHGEPEPELLALRSALHRQLEHPQAAAADARAALADPRLSVSS
                     QIDLLLPSDPAQARAVFAAAQHSPMMGELSDTDAAYLAVRVGDDASAAQAFDRAAQHG
                     QLPDHALLDAGYVAGRLGRSQVAVDYFKRAIDAAEAGRLALTPQRLFETRREVADRTR
                     TWGAILSLGYRGVSPGGQMAGTPTTLGDSLQAGMELYWRPAGYRDGSYYELYGGGFQT
                     PWAKGAAPSGGPTTQAMLGARVKPLSSLNLVLAIERRLKVGRLSSNDWLLRAGYSYTH
                     GTDLRIDADSWFTVQVYAEAGRFLRAHRSYATFEANLGRSIRLNDIDAQLVLFPHLVL
                     AADHDSKLAPGQRSASGAGIGVAARCWFNQDRYQAPRSYLDVSLQYRARLGGDARGKG
                     VFLRATLNF"
     gene            11548..11835
                     /locus_tag="PK242_04985"
     CDS             11548..11835
                     /locus_tag="PK242_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93403.1"
                     /translation="MKTLSTHPLRAGVSATLLLCSLAASATTVAPPPLPMGSAMAKPQ
                     VGASPTEEKRAEKAHKQPSVLRRNHLHDDSLAPPRQGAIPRATVSVPRDSK"
     gene            11875..13569
                     /locus_tag="PK242_04990"
     CDS             11875..13569
                     /locus_tag="PK242_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HOZ93404.1"
                     /translation="MKTLRYFVALLSALCMTAATAADTGFRISIRLPWLIAPSAPPPV
                     APQAGPRTLVLYDAPPGQTFTKLGMAYAIMMRNLLGHFDSQVDMVPVHDYVTGQVNNY
                     DATFYLGAYYDNQVPPAFLADVTTTTKTVVWFKYNLWQIAWNPAYDFQNRFGFGFSGL
                     RGLNALPSAANPAPGFFDTITYKSLPFVKYYDYAGGAVAADPDVGIVAVTDTQKATTL
                     VPMSNPKTSETAPYIVRAGNFWYVADLPFSYIGPRDRYLVLTDLLHDMLGVNHATSHK
                     AMVRLEDVGAMVSVSAMKSLTNYLYQRRIPFSIATIPYYADPLGTYNGGVPMFVPMSQ
                     ASNLKTSLNYALARGGEIVMHGYTHQYSNMRNKYTGVSGDDYEFWNIVDNTPVMEDSL
                     AWALSRYQAGLQELSSNGYTATAWETPHYHGSALASKAAAQTFAKTYQRVVYFTADQP
                     NFNAPVAKDFGVGQIFPYPIKSDYYGQRVLPESLGNIEYDISSIDPTSNYNYTWQDIV
                     TNAQYVKTVRDGYASFFFHPFWLEPALGVPGLADFKKVIQGISNLGFTWTVPSQIN"
     gene            13733..14545
                     /locus_tag="PK242_04995"
     CDS             13733..14545
                     /locus_tag="PK242_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93405.1"
                     /translation="MLPWSAAAAPAARRLTGMVWQPSRQTLQPRGNWAQLGIRRLLVQ
                     WTAVDNLSFLPHAGLALQPPPLPDWDRIAGEPWAQEVIVGLAGLHAEPLARAALPALV
                     AQSRALCVAAAPWPLRIGGWYFPVEIDPGWQPPADLPERLNALPRPLWVSVYDSANLG
                     PHALLAWIERWLPTDVGVLFQDGVGVHAREPALARQYLIQLGARLGRTRVQVIAEAFR
                     PAPGGGFRSATAQEFLPQLDAYQDWPILAFDGPHYLNEALIQDLVAAGVRPA"
     gene            complement(14607..15293)
                     /locus_tag="PK242_05000"
     CDS             complement(14607..15293)
                     /locus_tag="PK242_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOZ93406.1"
                     /translation="MDIPAFALAFGKSLLFALAAVLPILNPPASAPVFVNLTRGLDHD
                     TRAMLARRIGRHVVMLLAGAMLVGSYVLDFFGISLPIVRVAGGLIVASTAWRMLHVQH
                     LAQDDKAQLAQSLTADNVRIQAFYPMTFPLTCGPGSIAAAITVGASLHSRRVTEALVN
                     FAGGLAATLLVGLLVTLTFRYAGRLLRPLGEVGQIVFLRLMAFMLLCVGVQIVWDGAS
                     ALLQGLGNSK"
BASE COUNT         2308 a         5528 c         5193 g         2192 t
ORIGIN      
        1 agcagggccc gaccaccacc accagccggt cgtcctggcc gtgcagcacg cgcgacagcg
       61 ccgtgcggct ggcttccacc aggtcctgcg cggcgtccgg ggcaggcagc cattcttcca
      121 gcagggccgg cgtcagcagc gggcgcacgg cgcggatgcg ggtgtcgtcg atgcgggtgg
      181 tgtcgtgggt ggaggggttt tcgatcggga gcatcggccg attatcggtg ttttgcgatt
      241 gcaggcagcg cgcagcaagc gccgccagct atcaatttga tattcgaccg gcgtcggacg
      301 ccgggcccgc ctcgggcgcc tgggccggcc ggcgccgcat gcggatggcg ccgaacgggc
      361 agcgcagcgc acacagggcg cagccggtgc agccggccgc gtcgtccagc gccgaggcct
      421 tgcgcccgcc gtcggcgtgc agcgacagca catgcggcgc gcacaccgcc acgcaccagc
      481 cgcagccggt gcagcgtgtc gggtcgatct gcggcagggc cgcgcggcgc ggcgcggcgg
      541 tcatgccagg gtcatcacag cgcacccagc ggcgccaccc cgatccaggc cgcgtgcagc
      601 cagaacgcga acgccaccca ggccagcgcg cccaccagca gcgtcagcag cgtggcgccc
      661 caccggcccg gtggggcggc cgtgccctgg gcgcggtcgc ggcgcatcgc ggcgcgccag
      721 tcgagcagcg cccacagcaa aaaggcgccg aacagcagca ggccggcgcc gctgccgctg
      781 gccagcagat ggcccagcgc ccacagcgcc acgcccagca ccatggggtg gcgcaggcgg
      841 gccttgacga gattgcccgg cacgtaggcg gccgccagca gcacgaaggc ggccagcgtg
      901 aacagcgcgt tcaggtgccg cagaccggcc ggcggcaccc accacaccac cggctggcgc
      961 cgcgccaggg cccaacccca gacgatcagc gccaggccga gcagcgacag cagcgcatac
     1021 agccccttcc agcgccccgc gcccaggcgc gcgatgccgc gcgtgcgcca gccgtcggcc
     1081 acgatgcgca ccgaatgcac gcccagaaac agcagcagac cgagcaccag cagccacatc
     1141 gcagacctcc ttggggcggc cgggcgcgcg ggcccggcgc cggcatggca gagcgaacca
     1201 ttatggaatc gaacgcggcg cccgtgcgaa gatcgcgcac gcccgccgcc ccaggagcat
     1261 cgtcttgacc gcccccgccc cgcccccggc catcaccctc atcggcgcgc ccaccgacgt
     1321 gggcggcggc gcacgcggcg ccagcatggg accggaggcg ctgcgcgtgg ccggcctggt
     1381 cgaggccttg cgcgccctgg gtgccgacgt gcaggatgcc ggcaacctgg ctggcccgcc
     1441 caaccccggc ctgccgccgg tgcagggctt ccggcacctg gccgaggtga cggcctggaa
     1501 ccaggcggtg caccaggccg tgcaggcctc cctgcaggac ggccggctgc cgctgctgct
     1561 gggcggcgac cacagcctgg ccattggctc gatcagcgcc gtggcgcgcc attgccgggc
     1621 ccggggccag cccctgcggg tgctgtggtt cgacgcccac gccgacttca acaccgccgc
     1681 gctcacgccc agcggcaacc tgcacggcat gccggtggcc agcctgttcg gcgacgggcc
     1741 gccggcgctg gtgggtctgg gcggcttttc ggccgcccag ccggcgctgc gcgcgcacga
     1801 attgcgccag atcggcatcc gcagcgtcga tgcgggcgag aagcgcctgg tgcacgaggc
     1861 cggcatcgag gtgttcgaca tgcgcgccat cgacgagctg ggcatgcgcg cggtgatggc
     1921 gcgctgcctg gccgggctgg atgccggcag ccacctgcac gtcagcctgg acgtggactt
     1981 tctcgatccc gacatcgccc ccggcgtggc caccaccgtg cgcggtggtc cgagctaccg
     2041 cgaagcgcag ctgtgcatgg aaatggtggc cgacaccggg cgcctgggct cgctcgacat
     2101 cgtcgagctc aacccggcct gcgacagcca caaccgtacc gccgagctgg cggtggatct
     2161 ggtggaaagc ctgttcggca aaagcacgct gatgcgccag cgcgggcgcg gctgaggtgg
     2221 ctgcgcgcgc aggccgtggc tgatgaacct ggaaacggca gttgactgct tgtcaactgc
     2281 ggtttccagg ttcaagcatc aaatcagact ctggcgctta tccagaatgc gccagcagct
     2341 attgttgtga tagcaaatga ctggttgccg aaggctgctc aggcagcgcc cgcggtggtg
     2401 tggcgggcga tgaagtgctc cacctgcggc gccggcatgg gcggtgccag cagaaagccc
     2461 tggaacccgt cgcagccgcg ctggctcagc aagcgctgct gcgcctcggt ctccacgcct
     2521 tcggccacca ccttcaggcc caggctgtgc gccatggcga tgatggcgct ggcgatggcg
     2581 gcgtcgccgg agtcggtcag cacgtcggcg acgaaggcgc ggtcgatctt gagctggtcc
     2641 agcggcagcc gcttgaggta ggccagcgac gagtagccgg tgccgaagtc gtccagcgac
     2701 agcgccacgc cgagctgccg caggcggtcc atctgcgcca gcgtgatgtc caggcggtcg
     2761 gccagcagac tctcggtgag ctccagccgc aggcgcgcgg cgggcgcgcc gctctggcgg
     2821 atgacggccg tcacctgatc gacgaagtcc gggtggcgga actgctgcgc gctgacgttg
     2881 accgagacgc tcagagcggc cgtgtgcggc tgcgcctgcc agcgcgccag ctggcggcag
     2941 gcctgggtca gtgcccacag gcccaggggc acgatcaggc cgctgtcttc ggccacgggg
     3001 atgaactccg tcggccccac caggccgcgc tgcggatgct gccagcgcag cagggcctcg
     3061 acaccggtga tgcgccgcgc gcggtcgaac tggggctgga aatgcagctc cagctgccgc
     3121 tccgcaccca gcgccgcgcg caggtccgcc accagtgtgg tgctggcgtt gagcgccgcc
     3181 tgcatcgccg gatcgaagaa cgccaccgtg ttgcgcccca tgtgcttggc ctggtacatg
     3241 gccaggtcgg cctgctgcag cagctcggcc acgctgtccg ggccggcacc aaacagcgtg
     3301 acgccgatgc tggtggtgca gtggtgccga tggccgtcga ggtcgaacgg ctcgatcagg
     3361 cgcgccagca ccttgcgggc caccgcctgc gcctggcgcg ccgcctgcgc cgggtcgcgg
     3421 tccagcgccg gcagcagcac cacgaactcg tcgccgccga ggcgtgccac cgtgtcgccc
     3481 tcgcgcacac cgccgcgcag gcgcgcggcc acgccgcgca gcagcaggtc gccggtgtgg
     3541 tggccacggg tgtcgttgag caccttgaag tggtccaggt cgatgaacat caacgccccc
     3601 gtcgggccct ggcctgcgca ggccgcgcgc gccgcctcca gccgctcgat caacagctgg
     3661 cggttgggca ggccggtcag cgggtcgtag aacgccaggt ggtggatctt ggcttcggcc
     3721 gccttgcgct cgctgacgtc gcgcaggtac accgccagcc cctcggcgta gggataggcg
     3781 cgcagctcga tccacttgcc cgccgccggg tagaagtcct cgaactcgac gcgctgatcc
     3841 tgccgcagcg cctcatggac gcgcgcctgc agcagccccg gctgctggcc ctgcaggtgc
     3901 tgccagatcg gctcgtccag cagctcccgg ctgctggcgc ccagcagccg ctcgctttcc
     3961 tcgttcacat acatgaagcg gcccgcgcga tccagcgtca cgaaggcctc ggtgatgctg
     4021 gccagcgtgg tggtcagccg catcgccagg cgcagggttt cgtcctgcgc ctgcttgcgc
     4081 gagctcacgt cctgcactgc gccctggatc gccgtgcctg ccgcggtggc cgcgccgtcc
     4141 gcctcgccca ccacgcgcag ccagcgcctg ggttcctcgc cgggcgccag ctgcacctcg
     4201 acgtcgatcg gcgcaccgcc ctgctggcag ctcagcaagg cacgcgccag gcgctgcgcc
     4261 gattcttccg ccaggcgcgg ccacaagtgc tcgaccgtga tcgaccccgg caccacgccc
     4321 agcagcgcgg ccgcgtcgcc gctcagccag aaccgctgtg tggccgggtc gtacgcccac
     4381 tcgcccacct gcagcgcccg catcaggcgc gcccggaggt gccgcgccgc gtcaggcatg
     4441 cgcgcctcgc gcgcaacgac accacggcct gccgcggtcg cgcggccaca ccgaccgatg
     4501 accggtcgca accgccagga cgagcgcgta cctcatgtgc ggatcatccg ccagaacgtc
     4561 tttgtcgcgc aatgcatcga ttgtttcttt cagttgcatg tcgccaagat caagggatgc
     4621 gccagcgctg acgccacgtt gcgttggccc cgcggcggtc cggcaacccc aatccgggtg
     4681 aattgctccg gagcggcgct tccgaaatag tctgaaacac gtcgccttga tccgttgcgg
     4741 gcgactgcag ggagatacaa attgaacgtc tttcaggaat ttcgccgctg ccgtgacctg
     4801 ggcaccctca ggtcgcgcgt gaccgagctg agcgccaggt tcggcgcact gagccgtctg
     4861 gacatcctga ccgccgtgca tgccggccga cagcaggcca tctgcttttt gcgcatgcag
     4921 accgccgaac aggagcggac gctgatgaag acgctgggcg tcgggcgctt tggcggcgaa
     4981 atcgtgttcg tggtcgatct gccggaaccg acgggtttcg acgattcgct gccctcgtcg
     5041 cagtgggccg acagtgaggt gcagtgatgg acgcccgcgt ccgcgaccgc aaccgcggcg
     5101 gtgtggctgc cgccgcctcg atcctgatcg tctgtggcac ccggccggag atcatcaagc
     5161 tggcaccgct ctaccacgaa ctcaggcgcc agccctgggc ccatgtggtt tggctgcaca
     5221 ccggccagca cgcgcagatg gcccagccca tactggacag cttcgacatc cggcccgacc
     5281 tcaccctgac gcgccagggc gattcgctgc tcgacttcag cctcggctgc cgcgcccagc
     5341 tggaggcggt gctggcgggc ggccgctggg cggcggtggt ggtgcagggc gataccgaaa
     5401 gcgcctttca gggcgcgctg gcggggttct accacggcgt gccggtagcc catgtggagg
     5461 ccggcctgcg cacctacaac ctgaaccggc ccttccccga agaagggctg cgccagatga
     5521 tcggccgcat tgcgcgcctg cactttgcgc ccacggcgcg ttcgcggcag gcgctgctgt
     5581 ccgagggcgt gccgcagcag cgcatctggg tcaccggcaa caccgtcatc gatgcccagc
     5641 aatgggtgtg cgcacaccgc ggcatcggcc gccgggtcaa cggtcgtggc cacctgctgg
     5701 tgaccatgca ccggcgcgag aactgggggc aggacatcga gcgcgtgtgc cgcgccatcg
     5761 cccgcatcgc ccgcgaacag cccgatctgg aggtgctgtt tccggtgcac ctgaatccgg
     5821 tggtgcacgg gccggtgcac caattgctgg ccgggctgcc caacgtccgg ctgacggcgc
     5881 cgctggacta tctggaaatg cagcaggcgc tgcgcgacgc ctggatcgtg ctgaccgact
     5941 cgggcgggct gcaggaagag gccccgacct tcggcattcc cctgctggtg ttgcgcgagg
     6001 aaaccgagcg ccccgaagcg ctggaagcgg gctgcgcggc catcgtcggt accgcgaccg
     6061 acaccatcgt ggaacaggtg cggcgcctgt ggcgcgagcc ggccgagttc ctgcgcatgc
     6121 agcgggccgg caacccgttc ggggacggca cggcgtcccg gcagatcgcc cgccatctgg
     6181 cccgcgagct cggcgttcac gccagccatg ccggtgcccg acaggagcag gcgcatgtgg
     6241 tgggttgact acgtctcgag ctatctgtac gggctcaagt ccgtggccct ggcgctggcc
     6301 gtgatcatcc tggtcagcag cctggacgac ctgttcatcg atctggccta ctggtgccgc
     6361 cgcctgtggc gcagcgccac catctaccgg cgccacgcgc gcgcgcgacc ggccgatctg
     6421 caggcgcgcg acgaaaagcc gatggccatc atggtgccgg cctggcagga gcatggggtg
     6481 atcgaccgca tggccgaact ggcggccacc acgctggact acgagaacta ccacatcttc
     6541 gtcggcacct acccgaacga cccgcagacg cagtcggacg tcgagaaggt gtgcgcccgc
     6601 ttcccgaacg tgcacaaggt ggtcggcgcc cgggacggcc cgaccagcaa agccgactgt
     6661 ctgaacaatg tgctggacgc catcttccag ttcgagcggc gcgccggcat ccatttcgag
     6721 gggttcatcc tgcacgattc ggaggacgtg ctgtcgccga tggagctgcg ggtgttcaac
     6781 catctggtgg gccgcaagga cctgatccag ttgccggtct acccgctgcc gcgcccgtgg
     6841 accgacttca ccagctgcca ctacctggac gaattcgccg agtcgcatgg caaggatctg
     6901 ctggtgcgcg aggccctggt cggccaggtg ccgagcgccg gggtcggcac ctgtttcagc
     6961 cgcaaggccg tgatggccct gctcagccac ggcaagggca ttgcgttcga cgtcaagagc
     7021 ctgaccgagg actacgacat cggcctgcgc ctgaagggca tgggcatgca ggaggtgttc
     7081 gtgcgcttcc cgatgaagcc ccaccacgcg cgcgacgccg gcgccccgct cgggcagaac
     7141 gcccgcgagg ccagcacggt gtgcgtgcgc gaatacttcc cgtcggtgac ccgtgccgcc
     7201 attcgccaga aatcgcgctg gatcatcggg atcatctacc agggcaccaa gaccatgggg
     7261 tggtcacggc actggggcct gaactatttc ctgtggcgcg accgcaaggg cgtcatcacc
     7321 aacttcgccg gcatgctggc caccctggtg ctgctgcagc tggtggccct gtggctctac
     7381 cagcggctgg tgaccgacag ctatcagttt ctctccatct tcgaagacag cgcctggctg
     7441 acgctcctgc tggtggccaa tctcgtgctg ctgtgcaacc gcgccgtgca gcgcatctat
     7501 ttcgtgtcgt cctactacgg catccggcag ggcctgctgt cgctgccgcg catggcctgg
     7561 ggcaacgtga tcaatttctt tgccaactgg cgcgcgctga tgcagaccct gcaagcccag
     7621 gatgtgcggc gcatcgcctg ggccaagacc gagcacgact tccccaccct gggcgatgaa
     7681 ggcggcgccc gcgagccgct gggacagatc ctggtggcgc agtcggcact gacccaggaa
     7741 cagctcgatg aagcgctggc cgagccggtg ccggggctgc ggctgggggc catgctggtg
     7801 catcgcggcc tggtcacccc ggagcagctc gcccaggcgg tggccacgca gtcgcgcgcc
     7861 gactgggagc ctttcgacag ccgtcagctc ggccccgagc tggtggcctg cgtgcctgcg
     7921 gcggtggcgc tgcactatgc cgtgctgccg atgcggcaga ccgaggacac gctggtgctg
     7981 gcgtgcgagt cgtacctcga cccggtttcg ctcgccgcca tcgcccgcaa gctggatcgc
     8041 aaggtcagct acgtgatcgt gcccaagggc gaggtcacgg tggggctgcg acgctggtat
     8101 ggccgtgacg gcgatctggt cgccgcggat gcaggccgtg gcggcgtcca tgccacaccg
     8161 cagaccgcgg cgctgatgtg gcgcgtctat gtcgggggac agctgctgct gggcgacatc
     8221 ctgtgtgcgc tgggccatgt cgatccggcg gcgctgaaat cggtgctgat ccagcgcgcc
     8281 aagaccgggc agcgtctggg cgaattcatg gtggcgcgtg ggctgctgac gccgcaggtg
     8341 ctcgacgagg cactggcact gcagcgatcg ctccagccct cgatcgacac cttgctgggc
     8401 ggcctggtgc cgggccgcgt cggcagcggc ccgctggcga cggaggcatg aatgaagcgc
     8461 ctgtcgccct ccctggccct gacgctgctg ctggccgcgc tgccggcgct gggtcaaccg
     8521 gtcgatctgg ggccgggggt gtcgggctac acccgctttc tggtctatcc gcacctgcaa
     8581 aagggctttc aggccctgcg ggaggcccag cagacccttg cctacgaaga gttcgagcag
     8641 gccctgaaac tggcgcccga cagcgccgtg atcgcgggct acctggtcga agcctatcgc
     8701 catttcggtg accacgacaa ggcgcgccag ttgctgcagc ggcagctcgc ccgccatccc
     8761 gacgaccccc gcctgcgtca ggccctggcc agcctcggcc cggccgcgcc gacccggccg
     8821 cctgcaccgc aggtgacggc gggcggggcg ggcgaccggg cggccgagct tgccgccgcc
     8881 ccagcgccag cgccagcgcc tgcgcctgcg gtcgaggccg cgccgccacg cgcgcgcgct
     8941 gcggcgaccc ccgatgccgc atcggccgac gcacgccaga ccagccgtcg tgaccgggcg
     9001 tcatcggccg gcggccgccc gccgtcacgc tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnngaccagc cgtcgtgacc gggcgtcatc ggccggcggc cgcccgccgt
     9181 cacgctcggc cccagccacc acacgcctgg ccgcgctggc cccgcctgcc atgccaccgg
     9241 ccaaccaggc gccgcagcac ggcgcccgcg cggcctacgc gctggccgac gccgcctaca
     9301 aggccagcgc cacgggcgac tttgccgccg cggcgcgtga cgcacgccag gcgctgcaac
     9361 tgcagccgga caacgccgac taccgccgtc tgctggccta cgccttgctg gaaaccggcg
     9421 cctacgccga cgtggaagcg ctggccggcg agcgctccag ctccgacccc gccctgctgg
     9481 cactggccga tcaggcgcgt cggcagcgcg cctacgtcga gttcgaggcg gccagccggt
     9541 cgctggccgc cggcgatgtg gatggcgccg tcacgcaggc ggcgcgcggc gcccagctcg
     9601 cgcccgccac actggctcag caggtgcagt ggctgggtgc cctggcggcg gcgcagcgct
     9661 ggtcggcgct ggacgacgcg gccagtcagg tgctgcaacg tcctggcatg gacgacgccg
     9721 acatccggtt gctgcgcgcg catgcgcgcc agcgtctggg ccgcctggac gacgccaacg
     9781 ccgactacga ccgcgccctg gcgctctcgt ccgaccccga cacgcgcagc cgcaacgccc
     9841 gcctgatcgt ggccgacgcc gccctggcag ccggccagac ggtgcgtgcc gagcagctgc
     9901 tggccgcgct gtcgggaccg gccgacgccg ctgtcgacgc ccggctccgg caggccagcg
     9961 ccggcgcgcg ccgcaccctg ctgcccagcc ccaccgtggt gcccgccctg gccacgccca
    10021 gggtggtctg cgtcggctcg gcgcacaccc cctattgcga gctgtggccg ggcgacgccc
    10081 cggccgatcc gggcgccgtc catgccgatg ccgcgctgcg cgcctatgcg gcacggtcct
    10141 atgcggtggc ggcgacgcag gcgcgcagcg ccgtcgagca atcgcccgcc aaccggcgct
    10201 accaactgct gctggtgcgc gccctgctgg ccgccgacca gccgcagcag gcgctggagc
    10261 gcgccaacga attcctgtcc cacggcgaac ctgaaccgga gctgctggcc ctgcgcagcg
    10321 ccctgcatcg gcaactggag catccgcagg ccgcggcggc cgacgcgcgc gccgcgctgg
    10381 ccgaccccag gttgtcggta tcgagccaga tcgatctgct gctgcccagc gatccggcgc
    10441 aggcgcgcgc cgtgttcgcc gccgcgcagc actcgcccat gatgggtgag ctgagcgaca
    10501 ccgacgccgc ctacctggcc gtgcgggtgg gcgacgacgc cagtgccgcc caggcctttg
    10561 accgcgccgc ccagcatgga cagctgcccg accacgcctt gctggatgcc ggctacgtgg
    10621 ctggccggct gggccgctcc caggtcgccg tcgactactt caagcgcgcc atcgacgcgg
    10681 ccgaggcggg tcggctggcc ctgacaccgc aacggctgtt cgagacgcgg cgcgaggtgg
    10741 cggaccgcac ccgcacctgg ggtgccatcc tgtcgctggg ctaccgcggc gtttcccccg
    10801 gcgggcagat ggcgggcacg cccaccaccc tgggcgacag cctgcaggcg gggatggagc
    10861 tgtactggcg cccggccggc tatcgcgacg gcagctacta cgagctctac ggcggaggct
    10921 tccagacccc ctgggccaag ggtgcggcgc cgtcgggcgg gcccaccacg caggccatgc
    10981 tgggcgcgcg tgtcaaaccg ctgtcgtcgc tcaacctggt gctggcgatc gagcggcgcc
    11041 tgaaggtcgg tcggctgtcc agcaacgact ggctgctgcg cgccggctac tcgtacaccc
    11101 acggcaccga cctgcgtatc gatgccgaca gctggttcac cgtccaggtc tatgccgaag
    11161 cgggacggtt cctgcgtgcg caccgaagct acgccacctt cgaggcgaac ctcggtcgca
    11221 gcatccggct gaacgacatc gacgcccagc tggtgctgtt tccgcacctg gtgctggccg
    11281 ccgatcacga ctcgaagctg gcccccggcc agcgcagcgc cagcggcgcc ggcatcggtg
    11341 tggcggcgcg ctgctggttc aaccaggacc gctaccaggc cccgcggtcc tacctcgacg
    11401 tctcgctgca gtaccgggcc cggctgggtg gcgacgcccg tggcaagggc gtgtttctgc
    11461 gcgccaccct caacttctga tccggcctcg cccgcgcacg gcagccactc atgttccgtt
    11521 tgattgacaa ctgaaagggg atcgacgatg aaaacgctca gcacccatcc actgcgcgcc
    11581 ggtgtttcgg ccaccctgct gctgtgcagc ctcgccgcca gcgccaccac cgtggcaccg
    11641 ccgccgctgc ccatgggctc ggcgatggcc aagccccagg tcggcgcctc acccaccgag
    11701 gaaaagcgcg ccgagaaggc gcacaagcag cccagcgtgc tgcgccgcaa tcacctgcac
    11761 gatgactcgc tggccccgcc acgtcaaggc gccataccgc gggcgaccgt ttcggtgccc
    11821 cgcgattcga aatagaccgc ttggtcacgt cacgaacaca cccaggagcc gatcgtgaaa
    11881 accctcagat acttcgttgc actgctgtcc gccctgtgca tgacggcggc gaccgcagcc
    11941 gacaccggat ttcgcatctc catcagactg ccctggctca tcgcgcccag tgcaccgccg
    12001 cccgttgcac ctcaagccgg cccgcgcacc ctggtgctct acgacgcccc gcccggtcag
    12061 accttcacca agctgggcat ggcctacgcc atcatgatgc gcaacctgct ggggcacttc
    12121 gacagccagg tcgacatggt gccggtgcac gactacgtga ccgggcaggt caacaactac
    12181 gacgccacgt tctacctcgg cgcctactac gacaaccagg tgccgccggc cttcctggcg
    12241 gacgtgacca ccaccaccaa gaccgtggtc tggttcaagt acaacctgtg gcagatcgcc
    12301 tggaacccgg cctacgactt ccagaaccgc ttcggcttcg ggttcagcgg gctgcgcggc
    12361 ctgaacgccc tgccgtcggc ggccaatcca gcccccggct tcttcgacac catcacctac
    12421 aagtcactgc ccttcgtcaa gtactacgac tatgcgggtg gcgcggtggc cgccgatccg
    12481 gatgtgggca tcgtcgccgt caccgacacc caaaaggcca ccaccctggt gccgatgtcc
    12541 aaccccaaga ccagcgaaac cgccccctac atcgttcggg ccggcaactt ctggtacgtg
    12601 gccgacctgc cgttcagcta catcggcccg cgcgatcgct acctggtgct gaccgatctg
    12661 ctgcacgaca tgctgggggt caaccacgcc accagccaca aggccatggt gcgactggaa
    12721 gacgtgggcg ccatggtcag cgtgagcgcc atgaaatcgc tcaccaacta cctgtaccag
    12781 cggcgcatcc cgttctcgat cgccaccatt ccctactacg ccgatccgct gggcacctac
    12841 aacggcggcg tgcccatgtt cgtgccgatg tcgcaggcca gcaacctgaa gacctcgctc
    12901 aactacgcgc tggcgcgcgg cggcgagatc gtgatgcatg gctacaccca tcagtacagc
    12961 aacatgcgca acaaatacac cggcgtgagc ggtgacgact acgagttctg gaacatcgtc
    13021 gacaacacgc cggtgatgga agattcgctg gcctgggcgc tgtcgcgcta ccaggccggg
    13081 ctgcaggagc tgtccagcaa cggctacacg gcgacggcct gggaaacgcc gcactaccac
    13141 ggctcggcgc tggcgtccaa ggcggccgcc cagacctttg ccaagaccta tcagcgcgtg
    13201 gtgtacttca ccgccgatca gcccaacttc aacgccccgg tggccaagga cttcggggtg
    13261 gggcagatct ttccctaccc gatcaagtcg gactactacg ggcagcgggt gctgccggaa
    13321 agcctgggca acatcgagta cgacatcagc agcatcgacc cgacgtcgaa ctacaactac
    13381 acgtggcagg acatcgtgac caacgcccag tacgtgaaga ccgtgcgtga cggctatgcg
    13441 tcgttcttct tccatccgtt ctggctcgag cccgccctgg gcgtgccggg cctggcggac
    13501 ttcaagaagg tgattcaggg catcagcaat ctcggcttca cctggaccgt gccgagccag
    13561 atcaactgag caccctgcca cggcgcgccg cgggcgggcc gcatggccag tggccaccga
    13621 tcgacgcccg tggcagccct gtgatgccac gggtcttcac atggacaccg acctgccgcg
    13681 cacgccgccc cgcggccgcc acctgagccg gcgccaggtg ctggcggccg cgatgctgcc
    13741 gtggtcggct gcggcggcgc ctgcggcgcg ccgcctgacc ggcatggtct ggcagcccag
    13801 ccgccagacc ctgcagccgc gcggcaactg ggcccagctg ggcatacgcc ggctgctggt
    13861 gcagtggacg gcggtggaca acctgagctt tctgccccat gccggccttg cgctgcaacc
    13921 gccgccgctg ccggactggg accggatcgc cggcgagccc tgggcacagg aggtgatcgt
    13981 cggcctggcc gggctgcacg ccgagccgct cgcgcgcgcc gccctgccgg cgctggtggc
    14041 gcagtcgcgc gcgctgtgcg tggctgccgc gccgtggccg ctgcgcatcg gtggctggta
    14101 cttcccggtc gagatcgacc ccggctggca accgccggcc gacctgcccg agcggttgaa
    14161 cgccctgccg cggcccctgt gggtgagcgt gtacgacagc gccaacctgg ggccgcatgc
    14221 gctgctggcc tggatcgagc gctggctgcc gaccgatgtg ggggtgctgt ttcaggacgg
    14281 cgtgggggtg catgcgcgcg agccggcgct ggcacgccaa tacctgatac agctcggtgc
    14341 ccggttgggg cgcacccgcg tgcaggtgat cgccgaagcc ttccgaccgg ccccgggagg
    14401 gggctttcgc agcgccacgg cgcaggagtt cctgccgcag ctcgacgcct accaggattg
    14461 gcccatcctg gccttcgatg gcccgcacta cctgaacgaa gccctgatcc aggatctggt
    14521 ggccgccggt gtgcggcccg cctgaatcca gaaagctacg attttgatag ctgttggcgc
    14581 agttcaggcg ccgtcaagag gctgatttat ttcgaatttc ccaggccctg caacagcgcg
    14641 ctggcgccgt cccacacgat ctgcacgccc acgcacagca gcatgaaggc catcaggcgc
    14701 aggaacacga tctgcccgac ctcgcccagg gggcgcagca ggcgaccggc gtagcgaaag
    14761 gtcagggtca ccagcaggcc caccagcagg gtggcggcca ggccaccggc aaagttgacc
    14821 agggcctcgg tgacccggcg gctgtgcagg ctggcgccga cggtgatggc ggcggcgatc
    14881 gagcccggcc cgcaggtgag cgggaaggtc atcggataga aggcctggat gcgcacgttg
    14941 tcggccgtca gcgactgcgc cagctgcgcc ttgtcgtcct gcgccagatg ctggacatgc
    15001 agcatgcgcc aggcggtgga ggccacgatc agcccgccgg ccacgcgcac gatgggtagc
    15061 gagatgccga agaagtcgag cacgtacgac cccaccagca tggcgccggc cagcagcatc
    15121 accacatggc gcccgatgcg ccgcgccagc atggcgcgcg tgtcatggtc cagcccgcgc
    15181 gtgaggttga cgaacaccgg cgccgacgcc ggcggattga ggatcggcag cacggccgcc
    15241 agggcgaaca gcaggctctt gccgaacgcc agggcgaacg cgggaatgtc catggtggcg
    15301 ccgatgttac gccaaggctg c
//
LOCUS       DAOOOG010000041        14824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4305_length_14824_cov_28.089376, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000041 DAOOOG010000000
VERSION     DAOOOG010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14824
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4305_length_14824_cov_28.089376"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /gene="metG"
                     /locus_tag="PK242_05005"
     CDS             complement(<1..923)
                     /gene="metG"
                     /locus_tag="PK242_05005"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOZ93407.1"
                     /translation="MTARQLFVTTALPYANGKFHIGHIMEYIQADIWVRFQRMMGAEV
                     NFVGADDAHGAPIMIAAEKAGKTPEQFVADIAAGRQQYLDGFLIGFDNWHSTHSPENT
                     AISQAIYTDLKAAGLIESRTIEQFYDPQKGMFLPDRFIKGECPNCHSKDQYGDNCEVC
                     SAVYAPTELIEPYSALTGARPELRTSEHFFFRLSDPRCIEFLRGWTERLQPEVANKVR
                     EWIEPGADGQPKMGDWDISRDAPYFGIPIPDAPGKYFYVWLDAPIGYLASLKNLLDKR
                     GQDWAAYMANPALEQYHFIGKDIITFHTLFWP"
     gene            1126..2217
                     /gene="apbC"
                     /locus_tag="PK242_05010"
     CDS             1126..2217
                     /gene="apbC"
                     /locus_tag="PK242_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HOZ93408.1"
                     /translation="MTILEQDVRRAAATVLDPNTQQDFVSTGQLKNLQISGGDVSFDI
                     ELGYPARSQMPALRQALVAAVRTLGGVGNVSVNIVTRVVAHAVQRGVPLLPGVKNIVA
                     VASGKGGVGKSTTAVNLALALAAEGASVGILDADIYGPSMPIMMGLSGRPESLDGKTM
                     EPLTNHGVQVISIGFLVDPDQATIWRGPMATQALDQLLRQTHWKELDYLIVDLPPGTG
                     DIQLSLSQRVPLTGAVIVTTPQDIALADARKGVAMFEKVGVPILGLVENMAVHVCSNC
                     GHVEHIFGADGGKRYAADKGIEYLGALPLALEIREQADSGRPSVVADPDGQVATSYKA
                     IARQVAIRIADRAKDYSSKFPSITISRSS"
     gene            2400..3143
                     /locus_tag="PK242_05015"
     CDS             2400..3143
                     /locus_tag="PK242_05015"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HOZ93409.1"
                     /translation="MNSEIFNGWPGWLTQHSGPWLPLLSTGVALLALMLLWAMVRRSW
                     RRGRAARNYDASADRFGQTPAITERQVELLRYLQQAFGDGVVLYRPRLASFLAVRRTR
                     DRLAAQQRLLEQQVDFLVCADDGKPLFAFEVDAFKDPAAGGPDRDASEKNRMLKSAGV
                     RLVRLKGALASLPPPEILRMRLLAAQRTPVPAAEPAASDFKASGFASSGFAASGFAHS
                     RSADSGVMSLSALMALPDGEDPWSGVRKR"
     gene            complement(3302..3526)
                     /locus_tag="PK242_05020"
     CDS             complement(3302..3526)
                     /locus_tag="PK242_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HOZ93410.1"
                     /translation="MNGQTATLTPARRAADTTPAHHAPDRPRPAAEAAAPAPLPSEQL
                     LQGRKTIEISHNGTLYRLQATKLGKLILTK"
     gene            complement(3523..3942)
                     /locus_tag="PK242_05025"
     CDS             complement(3523..3942)
                     /locus_tag="PK242_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOZ93411.1"
                     /translation="MAGFIAQDHDEVMSEINMVPFIDVMLVLLVIFIITVPVMKHAVN
                     VQLPRAANEKEVVQPQTLRLTVQADGSYWLDGQKLADDQLGTRLQAAAAQQPQPDLHI
                     RGDRDVRYERVAQAMAAAQQAGLSKIGFITEPPQRTP"
     gene            complement(3948..4661)
                     /locus_tag="PK242_05030"
     CDS             complement(3948..4661)
                     /locus_tag="PK242_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOZ93412.1"
                     /translation="MNNEFGLLHVWAQGDWVTRSVFGALLLMSVASWVVIVLKALGLM
                     RTQRQAARVEGFWHSSDIAEGLAKLGTGEANPFRALAIEGREAAAHHRATQAQLHDSL
                     DVSDWISRALQGSIDATASRLQAGLAVLASVGSTAPFVGLFGTVWGIYHALMKIGAAG
                     QASIDQVAGPIGEALIMTALGLAVAIPAVLGYNALVRGNKTLLMRLGRFAHDLHAYYV
                     TGSRVNGSGDGKVVAMKKA"
     gene            complement(4669..5367)
                     /locus_tag="PK242_05035"
     CDS             complement(4669..5367)
                     /locus_tag="PK242_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOZ93413.1"
                     /translation="MSLPGRHTVVVGSVLLMHGVALWALHQGLLRPPVELIVPAQVLA
                     EFITPQPAPPPPAPPPPEPPKPTPAAVKPAPRPTPRPRPAPQPQPQPLAVQQAAPSAP
                     VMPIAPPEPPAPAPVAAPAPAPAAPPTPPAPPAPPAVVQPSSNAAYLNNPKPAYPAMS
                     KRLGESGRVVVRVLIGPDGRAQQAHIQRSSGYERLDQVALETARDRWRYVPGTRGGVP
                     EAMWFNVPINFVLE"
     gene            complement(5544..5768)
                     /locus_tag="PK242_05040"
     CDS             complement(5544..5768)
                     /locus_tag="PK242_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOZ93414.1"
                     /translation="MIVCVCNCISDKEIARQAHAGMGFDDIQLELGVATCCGRCEDFA
                     RDLVAQCSASRPVAALRDAGGASAGLMEAV"
     gene            complement(5986..7068)
                     /locus_tag="PK242_05045"
     CDS             complement(5986..7068)
                     /locus_tag="PK242_05045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HOZ93415.1"
                     /translation="MTTPPLTLADQAAQAERLLDPAVWAYLNGGAADELTLRANLTAW
                     QRLPLWPRVLRPLGGGHTRSVLAGRELAHPILLAPVALQRLAHPDGERASALAAAAQG
                     AGYVLSTQSSTPLETVAQAVLGDPGRGPLWFQLYWQADRGFNRALVQRAEAAGFEAIV
                     LTADAPVQGVRDRERRAGFVPPPGVRAVHLQGAPLAPPAADTYCAGLPAHAPNWDDLA
                     WLQGQTRLPLWLKGVLHPQDARQAQALGVAGLIVSNHGGRTLDSAPASADALVQVRAA
                     VQDALPLIVDGGIRRGTDVLKAIALGARAVLIGRPALHALAAGGALGVAQMLRLLRDE
                     LEMAMALTGCRTLADAGPALLTPTAA"
     gene            7215..7841
                     /locus_tag="PK242_05050"
     CDS             7215..7841
                     /locus_tag="PK242_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOZ93416.1"
                     /translation="MPVVVIANPKGGVGKSTLASHVAGYWASRGHAVMLGDVDRQQSS
                     RLWLSLRPEAASPIATWDVLRDFVVRPPRGTTHVVLDTPAGLHGSKLTELLRLADKVL
                     VPLQPSIFDIYATREFIDELLGARGARQAELALVGMRVDERTIAAEQLRQFTDGLGLP
                     VLGLLRPTQNYVHLAARGLTLFDIAPGRVARDLEQWQGICRWLDRSPA"
     gene            7882..8334
                     /locus_tag="PK242_05055"
     CDS             7882..8334
                     /locus_tag="PK242_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOZ93417.1"
                     /translation="MKHFETLAELAACVGQEVAVTDWFTVEQAHIDQFAQATGDHQWI
                     HVDPERAKAGPFGAPIAHGYLTLSMLAGHLGGVVQVREVRMGVNYGLNKVRFMAPVPV
                     GSRLRARVTLLDAQPLEGGMQLAWRSTIEREGADKPVCVAEPLARYYV"
     gene            8428..8757
                     /locus_tag="PK242_05060"
     CDS             8428..8757
                     /locus_tag="PK242_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HOZ93418.1"
                     /translation="MPHSADLIESMTETKDQLASNLRRVVSDTEDLLAATAEHTDSRI
                     AELRARARENLRIARDKLADADAVVRARARQAAAATDDYVHDNPWSSIGAAAALGILI
                     GVLLGRR"
     gene            8786..9169
                     /locus_tag="PK242_05065"
     CDS             8786..9169
                     /locus_tag="PK242_05065"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOZ93419.1"
                     /translation="MTSPAQDAGTAARLRALLADVVELVQVRLELFTVEAREELARLA
                     RMAVLGALAVVLLSFGLIFLALFLTVLLWDSQRLLALGVFTTLFLGGGAGLVLWVRQQ
                     ARQGLQMFAATRDELLRDQQRLRQP"
     gene            9166..9501
                     /locus_tag="PK242_05070"
     CDS             9166..9501
                     /locus_tag="PK242_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93420.1"
                     /translation="MNRPPTDDLRARREQLRLRSDQLREQIAARTQVLQPLFRASDRV
                     RSGVRGVQQARQGRAPYVLGLLALSALAGALLVRPRLATQLALRAWSGWQMYRRARPV
                     VERALRQWR"
     gene            9531..10022
                     /gene="nudB"
                     /locus_tag="PK242_05075"
     CDS             9531..10022
                     /gene="nudB"
                     /locus_tag="PK242_05075"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HOZ93421.1"
                     /translation="MSAHSCVGRCRFKIPESVLVVIHTPALEVLLIRRADVRQGEFWQ
                     SVTGSKDDPGEALTQTAVREVGEETGIDARAPGCLLRDWGIRNRYEIYPRWRHRYAPG
                     VTHNLEHVFGLCLPARAPVRLNPREHDAFEWLPWRAAADRCFSPTNAEACLLLPRFAG
                     APA"
     gene            10019..10798
                     /locus_tag="PK242_05080"
     CDS             10019..10798
                     /locus_tag="PK242_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOZ93422.1"
                     /translation="MREVHFGGAHQLLRVATYNIHKGVQGLGPARRLEIHNLALAIET
                     LDADIVCLQEVRAINRKEASYFTGWPELPQAEFLAPEGYDAVYRTNAVTRHGEHGNAL
                     LSRWPIIHERHEDMSDHRFEQRGLLHAELLVAGVSVHAIVVHFGLIPASRIRQTAQLK
                     AYIAREVPRGAPLIVAGDFNDWGTRVRRLLRMEALHSFEGPRTLTYPSRYPVAQLDHI
                     YARGLHPLSLAAPRGRVWSRMSDHLPLIAEFRMPHDALHKS"
     gene            10874..11599
                     /gene="folE"
                     /locus_tag="PK242_05085"
     CDS             10874..11599
                     /gene="folE"
                     /locus_tag="PK242_05085"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HOZ93423.1"
                     /translation="MKAQPEFPTPAGAEDEGTPVSVRIRQRLQAAQRRYHANDNIADC
                     IRPGELEQLLDEVTTRMEGVLDSLVIDTAGDHNTRDTARRVAKMYLNEVFKGRYVKAP
                     SITEFPNAERLNELMIVGPITVRSACSHHFCPVIGQLWIGVLPNEHTNVIGLSKYARL
                     AEWVMGRPQIQEEAVVQLADYIQSKTRPDGLAIVMQATHYCMAWRGVKDMDSKMINSV
                     MRGVFLKDMNLRREFLALIPQKS"
     gene            11605..12027
                     /locus_tag="PK242_05090"
     CDS             11605..12027
                     /locus_tag="PK242_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HOZ93424.1"
                     /translation="MLVRLLFASRAVDASREAIESILASARRHNPESGITGILLHGSG
                     VFLQGMEGGRQAVSDLYGTIQRDPRHKDVVLLHYEEILERRFGGWTMGLVDVGRVNAS
                     VLLKYSERATLDPYAVSGRMSMALLEELMATACIASHA"
     gene            12043..12924
                     /locus_tag="PK242_05095"
     CDS             12043..12924
                     /locus_tag="PK242_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOZ93425.1"
                     /translation="MSAQALALVLLAGLVHAGWNIVAKQSGGDARFAFFTSVLMALAW
                     APLGLWLGWQALPHWDAAAWACVAASGLLHVLYYVTLLRGYRVADLTVVYPLARGSGP
                     LLSSLVAVTLLGERLSPLAAAGIGAVVLGVFLIAGGPRLLRRRDDPQARARVRQGLRY
                     GLLTGVWIASYTVVDGYAVRWLALSPILLDYMGNWVRIGLLLPVALRDRAATAQLWRR
                     QWRGALVVALCSPVAYVLVLFAMQQAPLSLVAPAREVSMLFAALIGGQLLGEGDRGVR
                     VAGALCIAAGVAALGLG"
     gene            complement(13027..14715)
                     /locus_tag="PK242_05100"
     CDS             complement(13027..14715)
                     /locus_tag="PK242_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOZ93426.1"
                     /translation="MPPQLARHLPFLRWPRPTARLLQGEAMAGLTVGLMVIPQGVAYA
                     QLAGMPLITGIYASMLPALVAVLFSASARLSVGPTALTSLMIAAALTPLATPGSAEWV
                     ALAVWLALLSGLLQVALGLVRFGWLLNLVNAPVLMAFTQAAAVLIIGSQLPALLGFRA
                     GWADMLRQPAFDPLASAFGLGALALLLLGRRWKPTFPGVLVIVVAAAALSWALGFEAG
                     GGAVVGALPQGLPAPYLPPWPGWQLLGQLLLPTLVVTLVSFLETAASAKVDSQRKGER
                     WDQDQDLIGQGLAKIASGLCGAFATSSSFSRSALNLYAGAQTGWATVVSVAVVLLALL
                     LFTPVLRFVPQAVLAAIVVAAVLGLIKPREFLRLWRVSRVETVIAALTFAVTLAAAPA
                     LYWGVLTGVLMALSLFLYQRLHPRIIEVGLHADGSLRDRHLWQLAPLAPRTLALRLDA
                     ALDFASAGQFERHMTEYLAEHPTTRHVCLFAQPINRIDATGAEVFARVLGQLHERRVT
                     LLIVGLKLPAERVLRAAGALGEWPLLQMVRTDAEALAALARLEEPPADIAAAAI"
BASE COUNT         2126 a         5219 c         5331 g         2148 t
ORIGIN      
        1 ggccagaaca gcgtgtggaa ggtgatgatg tccttgccga tgaagtggta ttgctccagc
       61 gccgggttgg ccatgtaggc ggcccagtcc tggccgcgct tgtccagcag gttcttcagg
      121 ctggccaggt agccgatggg ggcgtccagc cagacgtaga agtacttgcc cggggcgtcc
      181 gggatcggga tgccgaagta cggcgcgtcg cgcgagatgt cccagtcgcc catcttcggc
      241 tggccgtccg cgcccggctc gatccactca cgcaccttgt tggccacctc gggttgcagg
      301 cgctcggtcc agccgcgcag gaactcgatg cagcgcgggt ccgacaggcg gaagaagaaa
      361 tgctcgctgg tgcgcagctc gggccgggcg ccggtgagcg ccgagtacgg ctcgatcagc
      421 tcggtcggtg cgtagacggc gctgcacacc tcgcagttgt cgccgtactg gtccttggag
      481 tggcagttgg ggcactcgcc cttgatgaag cggtcgggca ggaacatgcc cttctgcggg
      541 tcgtagaact gctcgatggt gcggctctcg atcagcccgg cggccttcag gtcggtgtag
      601 atcgcctgcg agatggcggt gttttccggg ctgtgggtgc tgtgccagtt gtcgaagccg
      661 atcagaaaac cgtccaggta ctgctggcgg ccggcggcga tgtcggccac gaactgttcg
      721 ggcgtcttgc cggccttttc ggccgcaatc atgatgggcg cgccatgcgc gtcgtcggcg
      781 ccgacgaaat tcacctcggc gcccatcatg cgctggaagc gcacccagat gtcggcctgg
      841 atgtattcca tgatgtggcc gatgtggaac ttgccgttgg cgtatggcag ggcggtggtg
      901 acaaagagct ggcgggcggt catggcagcg ggcgcgatgg gcgcggcaaa acccatcatt
      961 ttaggtgtcg ccccagccga agaagcgcgc gatggcctcg cgccgggccc gggcatggtg
     1021 cggcgaccgc gccgcgcagc gggccccttg gcgactcgcc acaagtacct gacagcgcga
     1081 aacactgcac tagactagcg gctgattcga caccgggcca acaccatgac catcctcgag
     1141 caagacgtgc ggcgcgcggc ggcgaccgtg ctcgacccca atacccagca ggatttcgtc
     1201 tccaccggac agctgaagaa cctgcagatc agcggtggcg acgtgtcctt cgacatcgag
     1261 ctcggctacc cggcccgcag tcagatgccg gccctgcggc aggcgctggt ggcggccgtg
     1321 cgcacgctcg gcggggtcgg caacgtgtcg gtgaacatcg tcacccgggt ggtggcgcat
     1381 gccgtgcagc gcggtgtgcc gctgctgccg ggggtgaaga acatcgtcgc cgtggcttcg
     1441 ggcaagggcg gcgtgggcaa gagcaccacc gccgtcaacc tggcgctggc gctggccgcc
     1501 gagggcgcga gcgtgggcat cctggacgcc gacatctacg gccccagcat gccgatcatg
     1561 atgggtctca gcggccgacc cgagagcctg gacggcaaga ccatggagcc gctgaccaac
     1621 cacggcgtgc aggtgatctc gatcggcttt ctggtcgacc ccgatcaggc caccatctgg
     1681 cgtggcccca tggccaccca ggcgctcgac cagctgctgc gccagaccca ctggaaggag
     1741 ctggactacc tgatcgtcga tctgccgccc ggcaccggcg acattcagct cagcctgagc
     1801 cagcgcgtgc cgctgaccgg cgccgtcatc gtcaccacgc cgcaggacat cgccctggcc
     1861 gacgcgcgca agggggtggc gatgttcgaa aaggtcggcg tgccgatcct gggcctggtg
     1921 gagaacatgg cggtgcacgt gtgttccaac tgcggccacg tggagcacat cttcggcgcc
     1981 gacggcggca agcgctacgc ggccgacaag ggcatcgagt acctgggcgc gctgccgctg
     2041 gcgctggaga tccgcgagca ggccgactcg ggccgcccca gcgtggtggc cgacccggac
     2101 ggccaggtgg ccaccagcta caaggcgatc gcgcgccagg tggcgatccg catcgccgac
     2161 cgcgccaagg actattccag caagttcccc tccatcacca tctcgcgcag cagctgagcc
     2221 ggtcgcacgc cctcagagtc gctactattt aaatagctgc tggcgcttgg tggttgggcg
     2281 ccagagcctg tttttgatcg gaatcctggc gcccatccgg cgcccgcgtc cctacaccat
     2341 tggggggatg gcgccccccg cgcgcgcggc gtaagctgcg ggctccaggg agtcggaaca
     2401 tgaactcaga aatcttcaac ggctggcccg gctggctgac ccagcacagc gggccctggc
     2461 tgcccctgct cagcaccggc gtggcgctgc tggcgctgat gctgctgtgg gccatggtgc
     2521 gccgctcctg gcgccgcggg cgcgccgccc gaaactacga cgccagcgcc gaccgcttcg
     2581 gccagacccc ggcgatcacc gagcgccagg tcgaactgct gcgctacctg cagcaggcct
     2641 tcggcgacgg cgtggtgctg taccggccgc gactggccag ctttctggcg gtgcggcgca
     2701 cgcgcgaccg cctggcggcc cagcagcgcc tgctggagca gcaggtcgac ttcctggtct
     2761 gcgccgacga cggcaagccg ctgttcgcgt tcgaggtcga cgccttcaag gacccggccg
     2821 caggcgggcc ggatcgggac gccagcgaaa agaaccgcat gctcaagagc gccggcgttc
     2881 gtctggtgcg cctcaagggt gcgctggcca gccttccgcc gcccgaaatc ctgcgcatgc
     2941 gcctgctggc tgcgcagcgc acgccggtgc cggcggccga gccggcggcc tcggacttca
     3001 aggcgtccgg ctttgcgtcg tccggttttg ccgcgtccgg cttcgcccac tcgcgcagcg
     3061 ccgactccgg cgtgatgagt ctgagcgcac tgatggcgct gcccgacggc gaggacccgt
     3121 ggtccggggt gcgcaagcgc tgatcggggt agcgcctgag cgctctgatt ttgatggctg
     3181 ttggcgcttg atgggcaagc gccgagcgcg ttttggcgtg agaaaaaaga gagctggctg
     3241 ggtgagggcc tggctggctc cgacaagcgc gtcggtcgga cgcgtcccac gatgaaccta
     3301 cttacttggt gaggatcagc ttgcccagct tggtggcctg cagccggtac agcgtgccgt
     3361 tgtggctgat ctcgatggtc ttgcggccct gcagcagctg ttcgctcggc agcggggcgg
     3421 gcgcggcagc ctcggcggct ggccgcgggc gatccggtgc gtggtgcgcc ggggtggtgt
     3481 cggcggcgcg gcgcgcgggc gtcagggtgg cggtctggcc gttcatggcg tgcgctgggg
     3541 tggttcggtg atgaaaccga tcttcgacaa cccggcctgc tgggccgccg ccatggcttg
     3601 cgccacgcgc tcgtagcgca cgtcgcggtc gccgcggatg tgcaggtcgg gctgcggctg
     3661 ctgcgcggcg gcggcctgca ggcgcgtgcc cagctggtcg tcggccagtt tctggccgtc
     3721 cagccagtag ctgccgtcgg cctgcacggt caggcgcagg gtctgtggct gcaccacctc
     3781 cttttcattg gcggcgcgcg gcagctgcac gttgacggcg tgcttcatca ccggcacggt
     3841 gatgatgaag atcaccagca gcaccagcat cacgtcgatg aacgggacca tgttgatctc
     3901 gctcatcacc tcgtcatggt cctgagcaat gaagccggcc atggtgttca ggccttcttc
     3961 atggcgacca ccttgccgtc gccgctgccg ttgacgcgcg agccggtcac gtaataggcg
     4021 tgcaggtcgt gcgcgaagcg gcccaggcgc atcagcaagg tcttgttgcc gcgcaccagg
     4081 gcgttgtagc ccagcaccgc cggaatcgcc accgccaggc ccagcgcggt catgatcagc
     4141 gcctcgccga tcgggccggc cacctggtcg atgctggcct gaccggcggc gccgatcttc
     4201 atcagcgcgt ggtagatgcc ccagacggtg ccgaacaggc cgacgaaggg cgccgtcgaa
     4261 cccaccgagg ccagcaccgc cagcccggcc tgcaggcgcg aggcggtggc gtcgatgctg
     4321 ccctgcagcg cgcgcgagat ccagtcgctc acgtccagcg aatcgtgcaa ctgcgcctgc
     4381 gtggcgcggt ggtgggcggc ggcttcgcgg ccctcgatgg ccagcgcgcg aaacggattc
     4441 gcttcgcccg tgcccagctt ggccaggcct tcggcgatgt cgctggaatg ccagaagcct
     4501 tccacccgcg ccgcctgacg ctgggtgcgc atcaggccga gcgccttcag cacgatcacc
     4561 acccaggagg cgaccgacat cagcagcagc gcgccgaaca cgctgcgcgt gacccagtcg
     4621 ccctgagccc acacgtgcag caggccgaat tcattgttca tggcttggtt actccagtac
     4681 gaaattgatc gggacgttga accacatcgc ctcgggcacg ccgccgcgcg tgccgggcac
     4741 atagcgccag cggtcgcgcg cggtctccag cgccacctgg tccagccgct cgtaaccgct
     4801 ggagcgctgg atgtgggctt gctgcgcccg gccgtcgggg ccgatcagca cgcgcaccac
     4861 cacgcgaccg ctttcgccca ggcgcttgct catggccggg taggcgggtt tggggttgtt
     4921 caggtaggcc gcgttggacg acggctgcac caccgccggc ggtgcaggcg gtgccggtgg
     4981 cgtcggcggc gccgccggtg cgggtgccgg ggcggccacg ggcgccggtg ccggtggctc
     5041 gggcggggcg atcggcatca ccggggcgct gggcgccgcc tgctgcaccg ccagcggctg
     5101 tggctggggc tgtggcgccg ggcgcggccg cggcgtcggc cggggggccg gtttgaccgc
     5161 cgccggtgtc ggcttgggcg gttcgggcgg gggcggtgcc ggtggcggcg gcgccggctg
     5221 cggggtgatg aattcggcca gcacctgggc cggcacgatc agctccaccg gcgggcgcag
     5281 cagcccctgg tgcagcgccc acagcgccac gccgtgcatc agcagcacgc tgcccacgac
     5341 gacggtgtgg cggccgggca gtgacatggc ggggggcggg gtgagcgagg cggaagacat
     5401 gatacgcagg cgggtcgttg ccgcgcaccg gcgcgcgggg cgctggcggc aacggcgaaa
     5461 caactaacgg cgcagcaccc agaaggcggc gcccagcacc aggcccaggg cgcccagggt
     5521 gggaagcagg gccgacacca gggtcacacc gcctccatca gcccggcgga cgctccgccg
     5581 gcgtcgcgca gcgcggccac cggccggctg gcgctgcact gggccaccag gtcacgtgcg
     5641 aagtcctcgc agcggccgca gcaggtggcc acgcccagtt ccagctggat gtcgtcgaag
     5701 cccatgccgg catgggcttg gcgagcgatt tccttgtcgg agatgcagtt gcagacgcag
     5761 acgatcatgg cgggctgggg cggggtggcc gagggttcgc atattaaatg cgattcattc
     5821 tcattgtcaa tacctttggg gaactgaagt gggcgcagcc gtcgcgccga ggcggttcag
     5881 gccagttcgc gtaccaccgg atggcgcacg cgcaccccgt catcgaagcc cggcgaggcg
     5941 attcgtcgct ggtcatcggc gatcggcaac ggcgccactc gcgcatcagg ccgccgtggg
     6001 tgtcagcagg gccggcccgg cgtcggccag ggtgcggcag ccggtcagcg ccatcgccat
     6061 ttccagctcg tcgcgcagca ggcgcagcat ctgcgccacg cccagcgcgc cgccggcggc
     6121 cagggcgtgc agggccgggc ggccgatcag caccgcgcgc gcgcccaggg cgatggcctt
     6181 gagcacgtcg gtgccgcgcc ggatgccgcc gtcgacgatc agcggcagcg cgtcctgcac
     6241 cgccgcgcgc acctgcacca gcgcgtcggc gctggcgggg gcgctgtcca gcgtgcgccc
     6301 gccgtggttg ctgacgatca gcccggccac gcccagggcc tgcgcctggc gcgcgtcctg
     6361 cgggtgcagc acacccttga gccacagcgg caggcgggtc tggccctgca gccaggcgag
     6421 gtcgtcccag ttcggggcgt gcgccggcag gccggcgcag taggtgtcgg cggccggcgg
     6481 cgccagcggg gcgccctgca ggtgcaccgc gcgcacgccc ggcggcggca cgaaaccggc
     6541 gcgccgctcg cggtcgcgca cgccttgcac cggggcatcg gcggtcagca cgatggcctc
     6601 gaagccggcc gcttcggcgc gctgcaccag ggcgcggttg aaaccgcggt cggcctgcca
     6661 gtagagctga aaccacagcg gcccgcggcc gggatcgccc agcaccgcct gggccaccgt
     6721 ctccagcggc gtgctggact gggtgctcag cacatagccg gcgccctggg cggcggcggc
     6781 cagggcgctg gcgcgctcgc cgtcggggtg ggccagccgc tgcagcgcca ccggcgccag
     6841 caggatcggg tgggccagct cgcgcccggc cagcacgctg cgggtgtggc cgccgcccag
     6901 cgggcgcagc acgcgcggcc acaggggcag acgctgccag gcggtgaggt tggcgcgcag
     6961 ggtcagctcg tcggcggcgc cgccgttcag gtaggcccag acggccggat ccagcaggcg
     7021 ttcggcctgg gcggcctgat cggccagggt gaggggcggg gtggtcatgc cgggttgctt
     7081 caaaaacaat agctgtttgc gcttgccaca caagcgccag agccggtttt tatcatggat
     7141 gtcgggcgcc cgggtggcgc cgtcggcagc cgctaccatt ggcgccggcg cgtttgcagc
     7201 aggaggaagt cgtcatgccg gtggtggtga tcgccaatcc caagggcggc gtgggcaagt
     7261 ccacgctggc cagccatgtg gccggctact gggccagccg cggccacgcc gtgatgctgg
     7321 gcgacgtcga ccgccagcag tcgtcgcgcc tgtggctgag cctgcgcccc gaggcggcgt
     7381 cgccgatcgc cacctgggac gtgctgcgcg acttcgtggt gcgtccgccg cgcggcacca
     7441 cccacgtggt gctggacacc ccggccggtc tgcacggcag caagctgacc gagctgctgc
     7501 gcctcgccga caaggtgctg gtgccgctgc agcccagcat cttcgacatc tacgccacgc
     7561 gcgaattcat cgacgagctg ctgggcgccc gcggcgcgcg gcaggccgag ctggcgctgg
     7621 tgggcatgcg ggtggacgag cgcaccatcg ccgccgagca gctgcgccag ttcaccgacg
     7681 gcctgggcct gccggtgctg ggcctgctgc gaccgacgca gaactacgtg cacctggcgg
     7741 cgcgcgggct gacgctgttc gacatcgcgc ccggccgcgt ggcgcgcgac ctggagcagt
     7801 ggcagggcat ctgccgctgg ctcgaccggt cgccggcttg acagcggcgg ccgtcgcggc
     7861 gggttacagt cgccgcccat gatcaagcac ttcgagaccc tggccgagct ggccgcctgc
     7921 gtcggacagg aagtcgccgt caccgactgg ttcaccgtcg agcaggcgca catcgaccag
     7981 tttgcccagg ccaccggcga ccaccagtgg atccacgtcg accccgaacg cgccaaggcc
     8041 ggcccgttcg gcgccccgat cgcgcacggc tacctgacgc tgtcgatgct ggccggccac
     8101 ctgggcggcg tggtgcaggt gcgcgaggtg cgcatgggcg tcaactatgg cctgaacaag
     8161 gtgcgcttca tggcgccggt gccggtgggc agccggctgc gcgcgcgggt gacgctgctg
     8221 gacgcccagc cgctggaagg cggcatgcag ctggcctggc gcagcaccat cgagcgcgag
     8281 ggcgccgaca agccggtgtg cgtggccgag ccgctggcgc gctactacgt ctgaacctgg
     8341 agcgcgccgc gcctgtgctc gccgccgggc gtttcgcgcc ggcgggcact ctgttagcat
     8401 gggtttttgc ctgcaaggag aagtctcatg ccccacagcg ctgacctcat cgagtccatg
     8461 accgaaacca aggaccagct ggcgtccaac ctgcgccgcg tggtgtcgga taccgaggac
     8521 ctgctggccg ccaccgccga gcacaccgat tccaggatcg ccgagttgcg cgcccgcgcg
     8581 cgcgagaacc tgcgcatcgc ccgcgacaag ctggccgacg ccgacgccgt ggtgcgtgcg
     8641 cgtgcccgcc aggccgccgc cgccaccgac gactacgtgc acgacaaccc ctggtcgtcg
     8701 atcggcgccg ccgccgcgct gggcatcctg atcggcgtgc tgctgggccg ccgctgagcc
     8761 cggcgtgcgc cggtacccgg ccgccatgac gtcccccgcc caagacgccg gcaccgccgc
     8821 gcgcctgcgt gccctgctcg ccgatgtggt cgagctggtg caggtgcggc tggagctgtt
     8881 caccgtcgag gcgcgcgaag agctggcccg gctggcccgg atggccgtgc tgggtgcgct
     8941 ggcggtggtg ctgctcagct tcgggctgat ctttctggcg ctgtttctga ccgtgctgct
     9001 gtgggacagc cagcgcctgc tggcgctggg cgtgttcacc accttgtttc tgggcggtgg
     9061 cgccggtctg gtcctgtggg tgcgccagca ggcgcgccag ggcctgcaga tgttcgccgc
     9121 cacgcgcgat gagctgctgc gcgaccagca gaggctgcgt cagccatgaa ccggccgccc
     9181 accgacgacc tgcgggcgcg ccgcgagcag ctgcgcctgc gctccgacca gctgcgcgag
     9241 cagatcgccg cccgcaccca ggtgctgcag ccgctgtttc gcgccagcga ccgcgtgcgc
     9301 agcggcgtgc gcggcgtgca gcaggcgcgc cagggccgtg cgccctacgt gctgggactg
     9361 ctggcgctgt cggcgctggc gggcgcgctg ctggtgcgcc cgcgcctggc cacccagctg
     9421 gcgctgcgcg cctggtcggg ctggcagatg taccgccgcg cccggccggt ggtggagcgc
     9481 gcgctgcggc agtggcgctg aacggcagcc gcggcgccca gggcgaaccc atgtccgcgc
     9541 actcctgcgt cggccggtgc cgcttcaaga tcccggaatc ggtgctggtc gtgatccaca
     9601 cgccggcgct ggaggtgctg ctgatccgcc gggccgacgt ccggcagggc gagttctggc
     9661 agtccgtcac cggcagcaag gacgaccccg gcgaggcgct gacccagacc gccgtgcgcg
     9721 aggtgggcga ggaaaccggc atcgacgcgc gtgcccccgg ctgcctgctg cgcgactggg
     9781 gcatccgcaa ccgctacgaa atctacccgc gctggcgcca ccgctacgct cccggcgtga
     9841 cgcacaacct ggaacatgtg ttcggcctgt gcctgccggc ccgcgcgccg gtgcggctga
     9901 acccgcgcga gcacgacgcc ttcgaatggc tgccctggcg cgccgcggcc gaccgctgct
     9961 tctcgcccac caacgccgag gcctgcctgc tgctgccgcg ctttgccgga gcgccggcat
    10021 gagggaggtg catttcggcg gcgcccacca gctgctgcgc gtggccacct acaacatcca
    10081 caagggcgtg cagggcctgg ggccggcgcg ccggctggag atccacaacc tggcgctggc
    10141 catcgagacg ctggacgccg acatcgtctg cctgcaggag gtgcgcgcca tcaaccgcaa
    10201 ggaagccagc tacttcaccg gctggcccga gctgccgcag gccgagtttc tggcgcccga
    10261 gggttacgac gcggtctacc gcaccaacgc ggtgacgcgc cacggcgagc acggcaacgc
    10321 cctgctgtcg cgctggccca tcattcacga gcggcacgag gacatgtccg accaccgctt
    10381 cgagcagcgc ggcctgctgc acgccgagct gctggtggcc ggtgtcagcg tgcacgccat
    10441 cgtggtgcat ttcggcctca ttccggccag ccgcatccgc cagacggcgc agctgaaggc
    10501 ctacatcgcg cgcgaggtgc cgcgcggcgc gccgctgatc gtggcgggcg acttcaacga
    10561 ctggggcacg cgcgtgcgcc ggctgctgcg catggaggcg ctgcacagct tcgaggggcc
    10621 acgcacgctg acctatcctt cgcgctaccc ggtggcgcag ctcgaccaca tctacgcacg
    10681 cggcctgcac ccgctcagtc tggcggcgcc gcgtgggcgc gtgtggagcc gcatgtcgga
    10741 ccacctgccg ctgatcgccg agttccgcat gccccatgac gcgctacata aatcatagct
    10801 gctggcgctt gccagacatg cgcaaacacc cattttcatt ggggattcca atgctgctac
    10861 catcgttgcg cccatgaaag cccaacccga attccccacc ccggccggtg ccgaggacga
    10921 aggcacgccg gtgtcggtgc gcatccgcca gcgcctgcag gcggcgcagc ggcgctacca
    10981 cgccaacgac aacatcgccg actgcatccg gcccggcgag ctggagcagc tgctcgacga
    11041 agtcaccacc cgcatggagg gcgtgctcga cagcctggtg atcgacaccg cgggcgacca
    11101 caacacgcgc gacacggcgc ggcgcgtggc caagatgtac ctcaacgagg tgttcaaggg
    11161 ccgttacgtc aaggccccca gcatcaccga attccccaac gccgagcgcc tgaacgagct
    11221 gatgatcgtc ggccccatca ccgtgcgctc ggcctgcagc caccacttct gcccggtcat
    11281 cggccagctc tggatcggcg tgctgcccaa cgagcacacc aacgtcatcg gcctgtccaa
    11341 gtacgcgcgc ctggccgagt gggtcatggg ccgcccgcag atccaggaag aagcggtggt
    11401 gcagctggcc gactacatcc agtccaagac ccggcccgac ggcctggcca tcgtcatgca
    11461 ggccacgcac tactgcatgg cctggcgcgg cgtcaaggac atggacagca agatgatcaa
    11521 ctccgtcatg cgcggcgtgt tcctcaagga catgaacctg cggcgcgaat tcctggccct
    11581 gattccccag aaaagctgag caccatgctt gtccgactcc tgttcgccag ccgcgccgtc
    11641 gacgcctcgc gcgaggccat cgaaagcatc ctggcctcgg cgcgccgcca caaccccgaa
    11701 agcggcatca ccggcatcct gctgcacggc agcggcgtgt ttctgcaggg catggaaggc
    11761 gggcgccagg cggtcagcga cctgtacggc accatccagc gcgacccgcg tcacaaggac
    11821 gtggtgctgc tgcactacga agaaatcctg gagcgccgct tcggcggctg gaccatgggc
    11881 ctggtcgacg tcggccgcgt caacgccagc gtgctgctca agtactccga acgcgccacg
    11941 ctcgacccct acgcggtgtc gggccggatg tcgatggccc tgctggaaga gctgatggcc
    12001 accgcctgca tcgccagcca cgcctgagcg gccgccaccc tcatgtcggc ccaggcgctg
    12061 gcgctggtgc tgctggccgg tctggtgcac gcgggctgga acatcgtcgc caagcaaagc
    12121 ggcggcgacg cccgctttgc cttcttcacc tcggtgctga tggcgctggc ctgggcgccg
    12181 ctgggcttgt ggctgggctg gcaggcgctg ccgcactggg acgccgccgc ctgggcctgc
    12241 gtggccgcca gcggcctgct gcatgtgctg tactacgtca ccctgctgcg cggctatcgc
    12301 gtggccgacc tgaccgtggt ctacccgctg gcgcgcggct cggggccgct gctgtcgtcg
    12361 ctggtggccg tcaccctgct gggcgagcgc ctgagcccgc tggcggcggc cggcatcggc
    12421 gccgtggtgc tgggggtgtt cctgatcgcc ggtggcccgc gcctgctgcg ccggcgcgac
    12481 gacccgcagg cacgtgcgcg cgtgcgccag ggcctgcgct acggcctgct gaccggcgtc
    12541 tggatcgcca gctacaccgt ggtcgacggc tacgccgtgc gctggctggc gctgtcgccg
    12601 attctgctgg actacatggg caactgggtg cgcatcggcc tgctgctgcc ggtggcgctg
    12661 cgcgaccgcg ccgccaccgc gcagctgtgg cgccgccagt ggcgcggcgc gctggtggtg
    12721 gcgctgtgca gcccggtggc ctacgtgctg gtgctgttcg ccatgcagca ggcgccgctg
    12781 tcgctggtgg cgccggcgcg cgaggtgtcg atgctgttcg ccgccctgat cggcggccag
    12841 ctgttgggcg agggcgaccg gggggtgcgc gtggccggcg cgctgtgcat cgccgccggc
    12901 gtggcggcgc tcgggctggg ttgaaagcgc tactgaatca atagctgctg gcgcttgctg
    12961 tgcctgcgcc ggaggcacat tcggcctggt tccgagttca ggattccgaa gccaagggct
    13021 caagcctcag atggcggcgg cggcgatgtc ggccgggggc tcctccaggc gcgccagcgc
    13081 ggccagcgcc tcggcgtcgg tgcggaccat ctgcagcagc ggccactctc ccagcgcgcc
    13141 ggccgcgcgc agcacccgtt cggccggcag cttgaggccg acgatgagca gcgtcacacg
    13201 ccgctcgtgc aactggccca gcacgcgcgc gaacacctcg gcgccggtgg cgtcgatgcg
    13261 gttgatgggc tgggcgaaca ggcagacatg ccgtgtcgtc gggtgctcgg ccaggtattc
    13321 ggtcatgtgg cgctcgaact gcccggccga ggcgaagtcc agcgccgcgt ccagccgcag
    13381 cgccagcgtg cgcggcgcca gcggcgccag ctgccacagg tgacggtcgc gcaggctgcc
    13441 gtcggcgtgc agaccgactt cgatgatgcg cgggtgcagg cgctggtaga ggaacagcga
    13501 cagcgccatc agcacccccg tcagcacgcc ccagtacagc gccggcgccg ccgccagcgt
    13561 gaccgcgaag gtcagcgcgg cgatcaccgt ctccacccgc gacacccgcc acaggcgcag
    13621 gaactcgcgc ggcttgatca gccccagcac cgccgccacc acgatggccg ccagcaccgc
    13681 ctgcggcacg aagcgcagca ccggcgtgaa cagcagcagc gccagcagca ccaccgccac
    13741 cgacaccacc gtggcccagc cggtctgtgc gccagcgtac aggttgagcg ccgaacgcga
    13801 aaacgacgag ctggtggcaa aggcgccgca caggccggag gcgatcttgg ccagaccctg
    13861 accgatcagg tcctggtcct ggtcccagcg ctcgcccttg cgctggctgt cgaccttggc
    13921 gctggcggcg gtttccagaa agctcaccag cgtcaccacc agggtcggca gcagcaactg
    13981 gcccagcagt tgccagccgg gccagggcgg caggtagggc gccggcagcc cctgcggcag
    14041 cgcgcccacc acggcgccgc cgccggcttc gaagccgagc gcccagctca gcgcggccgc
    14101 cgccaccacg atcaccagca cgccagggaa ggtcggcttc cagcgccgcc ccagcagcag
    14161 cagcgccagc gcgcccaggc cgaaggcgct ggccagcgga tcgaaggccg gctggcgcag
    14221 catgtcggcc cagccggcgc gaaaacccag cagtgccggc agctgcgagc cgatgatcag
    14281 caccgccgcc gcctgcgtga aggccatcag caccggcgcg ttgaccaggt tcagcagcca
    14341 gccgaagcgc accaggccca gcgccacctg cagcaggccc gacagcagcg ccagccacac
    14401 cgccagcgcc acccattcgg cgctgccggg cgtggccagc ggcgtcaggg cggcggcgat
    14461 catcaggctg gtcagcgccg tcggccccac cgacagccgc gccgaggccg aaaacagcac
    14521 cgccaccagc gccggcagca tggaggcgta gatgccggtg atcagcggca tgcccgccag
    14581 ctgggcgtag gccacgccct gcgggatcac catcagcccc accgtcaggc cggccatggc
    14641 ttcgccttgc agcaggcgcg cggtcgggcg cggccagcgc agaaacggca ggtggcgggc
    14701 gagctgcggg ggcatcaggt cagcatggta gcggggcggc gccggtcggc ggcgcgggcg
    14761 gctcgggcgt tgaacctaga aaccgcagtt gaccgcttgc aacccttggg aatgccgcat
    14821 gaac
//
LOCUS       DAOOOG010000042        14733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4348_length_14733_cov_18.041831, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000042 DAOOOG010000000
VERSION     DAOOOG010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14733
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4348_length_14733_cov_18.041831"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PK242_05105"
     CDS             complement(<1..454)
                     /locus_tag="PK242_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOZ93427.1"
                     /translation="MSINTVGIVGAGTMGNGIAQACAVAGIRVVMVDIAQAAVDKGLA
                     TVGKSLDRLLQKEKISEADKAAALGRIQGSTNYDDLKAAQLVIEAATENHALKNKILA
                     QLDALLAPEVIIATNTSSISITQLAAVTQRPDRFVGMHFFNPVPMMALV"
     gene            625..2244
                     /locus_tag="PK242_05110"
     CDS             625..2244
                     /locus_tag="PK242_05110"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HOZ93428.1"
                     /translation="MDYVIVGAGSAGCVLAARLSEDPHTQVTLLEAGPTDHSPLIHCP
                     AGIALMARTGQVNWGFETVPQAALNGRRGYQPRGKVLGGSSSVNAMIYTRGHPSDYDA
                     WAAAGNAGWGWDEVLPFFKLAEHNERGADAFHGARGPLNVKDLPEPNPFSLAFVEAGV
                     QAGYPRNPDFNGIEQEGVGLYQVTHRNGERCSVAKAYLTPHLGRPNLHVITGAHATRV
                     LIEGGRAVGVAYVRDGREEQVRAEREVLLCAGALQSPQLLLLSGIGPGEHLRQHGIAV
                     RHELPGVGGQLHDHPDVVQVWDVPGARELFGLSPAGAWRAARGLGQWRRERTGMLTTN
                     FAEGGAFLKSDPGLSAPDLQLHFVVGMLVNHGRSTVWGHGFSCHVCVLRPKSRGSVRL
                     ASADPLAAPAIDPALLSDPEDMRLMVRGFRQMRELLGHPALAQHGGRERSAAVRSDAE
                     IEAFIRRYADAIYHPVGSCRMGSGAQDVVDAQLRVHGLAGLRVVDASIMPSVVAGNTN
                     APVVMIAEKAAQMLRQGQAAQAREAVLEDVA"
     gene            2313..3177
                     /locus_tag="PK242_05115"
     CDS             join(2313..2723,2725..3177)
                     /locus_tag="PK242_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086896517.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOZ93429.1"
                     /translation="MGKAHANSWEVTDEFWKRVEPLVPQPARDPQRQYKRAAGAGRPT
                     KPPRLVFEAIMYVLRTGCQWKALPAERFGSASAIHQKFMVWSKAGFFESLWSAGLAEY
                     DDCEGIAWRWQSIDGAMFKAPLAQEAVGRNPTDRGKKGSKRHLLVDGRGVPLSLVVTG
                     ANAHDVTQLEAVLSFCMIKRPTPQQRRSKHLCADAGYRGKNAMKIMLAHGYIPHVVGR
                     KSEAECKKRDPKKLARRWVVEACHGWFNRFRKLLVRYEKLEHTFLALNHLAAAIIALR
                     KISLPINIIYG"
     gene            complement(3472..4839)
                     /locus_tag="PK242_05120"
     CDS             complement(3472..4839)
                     /locus_tag="PK242_05120"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOZ93430.1"
                     /translation="MSSSLLVVEDDPVLNRLLVKTLGKCGYDVDSAASWQQARERIAV
                     FAPDLVLLDVNLPDSTGFGPLSETAHSRSVVMLTAYGDVNQAVQAMRLGAADYLVKPV
                     NLDELELVIRGALERRQLLAQKAACGDGPGAMIGDSPAMRRLRQLIQEVGGSDVTVLI
                     QGESGCGKELVAQALHAASTRRDERFVAVDCTTLQETLFESELFGHERGAFTGADRRK
                     TGLIEAAAQGTLFLDEIGEAGPAIQAKLLRVLESGRFRRVGATTDLRTDARVVAATNR
                     DLAQRARQQLFRDDLYYRLAAFVIEVPPLRQRREDIAALAEHFIVQRRRGKGLPPLAL
                     GAEARQALERYDWPGNVRELRNVVERALILVGDAPRIAPAHLGLRSLPAPVAPAPAMA
                     VPAPATAEDESLVLQGEPTLEHIEREYLARLLKKYDGNRRKVADALGVSERTAYRMMD
                     RHGYK"
     gene            complement(4836..6812)
                     /locus_tag="PK242_05125"
     CDS             complement(4836..6812)
                     /locus_tag="PK242_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ93431.1"
                     /translation="MTASTAPDTPPSADTPPGRGGGLRAWIASSLSRQLTLGLVVALL
                     LSAGLLTALALAVSRGAVRAEHEAAAQRMAHLFEASLHNAMLKRDLDGLSTILSALGQ
                     VPGVTQARLAEPRGTVRFASRPQAVGGSAGEQLAGLCLTAGCAPPTPRLTWYPGEASE
                     RLHIAYPVLNQARCAGCHGAPAQHPVNGVLLLDFAPVATTGLGQGPAWLLLAVGLLSL
                     GLFAALMSLTLRRRVGRPLGQLARAADRLAGGELAARVDSRAPDELGRVSRSFDRMAE
                     RIDHTVGALREERRFLQTLIDALPDPVLVIGPDFRIRLANQAYARLLGHEMPAVIGQC
                     CHRVGRGLNEPCPSTLLTCPVAEMRLRPEPVHSLMTLRRADGGAVEVDIEAAPFTTGG
                     GERYTVEVMRPLDRSIRFSQQQRLSTIGLLANGVAHEIHNPLASIRLALQACLRGLRD
                     GDMPSEELIDYLRLVDTEIDRCVLTTQRLLQMSQAPSQELRPVRLETALGDVLALLGE
                     EFRARRVTLQLGGIAPEEQVLGDEAELRQVLVNLIHNALHAMPQGGQVEILAEPAEAG
                     ASLRLAVRDNGCGIAPQDLPLIFMPFFSRRVDGQRGTGLGLAICKATMERFGGGIEVS
                     SPPGQGSTFTLTLRRAPAALPDNPGPDLSSRPVP"
     gene            complement(6809..7927)
                     /locus_tag="PK242_05130"
     CDS             complement(6809..7927)
                     /locus_tag="PK242_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOZ93432.1"
                     /translation="MPAPQAAADPAQGASRLAIQAERCVHAQVLHAGCQACVTACPRQ
                     AWRAHPDGLGFDAEACDDCGLCVAACPTDALALPAPAPRVVTRPGGARTLLLACERAG
                     VAGAPGVTPCLYGLSPGWLLHAAEALSADTMALAPGDCAHCPRGGAGPGWRAHWLAVA
                     ERLRAAGRRVPALSPIAATAWPAPEPADAAAPRPGRRRFLGARAAPVGAALAALPPLA
                     GQRAWITAQLSAASPRAAPLWAVALDSARCTWCLACTRVCPSGALALERATAPGAPDR
                     FVLDMRRCTGCGLCHDVCTPQALSIPQALSTPRAPTEHEAAPGPDEVLLTRLSCPACR
                     VDFHQPAGAQREPTPCCPTCRQGRPRQHNRLVQQDCPP"
     gene            complement(7943..8650)
                     /locus_tag="PK242_05135"
     CDS             complement(7943..8650)
                     /locus_tag="PK242_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HOZ93433.1"
                     /translation="MNAVPASRPAWPALAAEDLRTLAWLHAKEHAPELLAELYANGFP
                     DTLVLVDADAPESRAMAVALRALASSRCDSEGELPYTDADQLAADYAAIYLTHTLRAS
                     PHESVWRDDDHLMLQAPTFSVREFYRRHGVQVPDWRQRADDHLTHELEFVALLLEREQ
                     AREAARFLKTHLMSWLPQFAGRVVQRADTPFYAALAALTLSACEACQQALPDVAVLPP
                     VVAQSAAVPPGGCGALS"
     gene            complement(8647..9384)
                     /locus_tag="PK242_05140"
     CDS             complement(8647..9384)
                     /locus_tag="PK242_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOZ93434.1"
                     /translation="MAEKQVINKLGIDAKARVKYAQDRRDATTYEPVQPEQQWGFIHN
                     NMDCIGCRACEIACKDKNGLPPGPRFRRVMYVEGGTFPEVFAYKVNMSCNHCAEPACL
                     PTCPTGAIWKRADNGVVDIDSSLCIGCRRCEAACPYGAPQFDPSDNTVKKCNLCIDEL
                     ESGRKPYCVSACMMRVLDIGPIDQLRAGTWDTKAMVKGVDVPVRQVQHMANPELTNPS
                     IVFVAHPKGKVESAPAVPAAPTPKASS"
     gene            complement(9397..12009)
                     /locus_tag="PK242_05145"
     CDS             complement(9397..12009)
                     /locus_tag="PK242_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOZ93435.1"
                     /translation="MKNDLSQGPGTRFPSVGESMDQVGLGYRRRDFLKVVGAGAGAAG
                     LVTLGVTVAPEKVEAFAYEPYFTDDQLTTVVTSCAHNCGSRHVLVAHKKGDVIVRLST
                     DNGEYQAQGAYGKDTFEEPQLRACLRGRSYRSRLYSPERLLYPMKRVGARGEHKFKRI
                     SWDQALDEIAQKMVQLKKDYGPTALLDQSYAGASYGVLHKSDQIEGLLGRFMGMFGCR
                     TNSWSVPSYQATTFSSRVTFGTIQDGNEDDTFAHSKLIIMWGWNPAYTFHGGNTFYYM
                     RMAKQRGCKFVLVDPQYTDSAAVYDAWWIPIKPNTDAAMMAAMAHHIFTANLHDQKFI
                     DKFVLGMDAGTMGQQFEGKQNFKDYILGKYDNTPKTPEWAEPICGVKAADIRKLADMY
                     AQTKPAALKASWAPGRASYGEQYNRMAAALQAMTGNIGVLGGCAEGVGKAWHSESVAY
                     PYDENANLWWASIKSDRWAHCVLNYPNLKREEIGLWPREDELDGKIPHIKGIFWQGSD
                     WFNQLTNINKEIQAIEKLELVVCMDSTITPSGIWADYLLPIATHFERHDVALPWYKGH
                     YYIHRPKVIEPLGESKTDFQVFTELSYRLQALDPGLENLGKRYNPRADRDYFQHPDAV
                     DEAYLKAWWEGSVQHHQGVKMSWEEFKKHGTYKFVLKEPHVAFRAQIAQGKPFETPSG
                     KIEIFSTQLAGITDWTKTQYGYEIPYIPKWIEPFESLNHPIAKQYPFHLISPHPRWRT
                     HSIFNNIPWLRETYEQEVTINASDARKLGIQTGDTVEVWNERGKCVVPAYVTERLLPG
                     VLVLFEGAWMDPDKNGVDRAGNPDFLTLDNPSPAGAFAYNNAMVQIQKSNLTHRPGWD
                     ELAVARSAVFRRDM"
     gene            complement(12006..12788)
                     /locus_tag="PK242_05150"
     CDS             complement(12006..12788)
                     /locus_tag="PK242_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93436.1"
                     /translation="MAGASASPTAAPAQPTDYHAAKWDPIHFKPAIDKATDAQCLSCH
                     AEVLKPSVRKSSPAGVEAATAKAWYQVTSTYQGEQDTFHRRHLDTAMAKQLMNMRCTT
                     CHQGNDPRDEAPGTSASNQNAELTLRKMINPETSCLKCHGKMNWEVMGLPSPWAQSKD
                     SFQNNCMLCHAAIRTMRHQVNYLNAQAIDEAGKTNGDVCFGCHGGRAWYRTHYPYPRH
                     AWPGMPTEVPDWAKGRPTESEVRFISAAVQPATTAAAQGASK"
     gene            complement(13013..13483)
                     /locus_tag="PK242_05155"
     CDS             complement(13013..13483)
                     /locus_tag="PK242_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93437.1"
                     /translation="MKAVRHVLGLSVVAAALVAALPALAQQQGMSGMSGQQGMSGMSG
                     QQGMSGMSGMSGQQGMSGMSGQQGMSGMSGQKGMSGMSGMSGQQGMSGMSGMSGNKAP
                     AKKKPAQQGMSGMSGMSGQQGMSGMSGMSGQQGMSGMSGQQGMSGMSGMSGDKK"
     gene            complement(13667..13999)
                     /locus_tag="PK242_05160"
     CDS             complement(13667..13999)
                     /locus_tag="PK242_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93438.1"
                     /translation="MTSTPLIALLLALVGAASWAQTSGPIAGLHPDRRPDGAPRVAQF
                     TRTDAQAAQALRGVEGVPPGNLRAIVSAGPWWVPMRHPGMPAPYDPRGLYGAAQPATL
                     AAPASAAR"
     gene            complement(14286..>14733)
                     /locus_tag="PK242_05165"
     CDS             complement(14286..>14733)
                     /locus_tag="PK242_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ93439.1"
                     /translation="SRAPRTLLALPVLLATGILGVMLALQISHSPKLLLVLPILLLAG
                     ITAASMALVFNAKAKGYDFFTYYFAVLLSPMMFLSGVFFPREQLPPFAHAIANWLPLS
                     AVVDLVRPLFLDQWPANAGRHLAVLAAYAIVGFWVALAFTRQRFRR"
BASE COUNT         2185 a         4866 c         5348 g         2334 t
ORIGIN      
        1 ccaccagcgc catcatcggc accgggttga agaagtgcat gccgacgaag cggtccggcc
       61 gctgggtgac ggccgccagc tgcgtgatcg agatcgacga ggtgttggtg gcgatgatca
      121 cctcgggcgc cagcagggcg tcgagctggg ccaggatctt gttcttcagc gcgtggttct
      181 cggtggcggc ctcgatcacc agttgcgcgg ccttcaggtc gtcgtagttc gtcgagccct
      241 ggatgcggcc cagggcggcg gccttgtcgg cttcgctgat tttttccttc tgcaacaggc
      301 ggtccaggct cttgcctacg gtggccaggc ccttgtcgac cgccgcttgc gcgatgtcga
      361 ccatgaccac ccggatgccc gccaccgcgc aggcctgcgc gatgccattg cccatggtgc
      421 ccgcgcccac gatgccgacg gtgttgatgc tcattgcgtg ttctccagtg atgagggaat
      481 tggggggtgc ggatcggcgc ctggccggcc gcccgtccag catggtagcg gctgcgcttg
      541 tcaacctgaa ttttggaagc tttcgcctaa gtctggcgcc ggcgggcgcg cacaatggct
      601 gagcatgaca aggagagacc ggacatggac tacgtgatcg tcggcgccgg atcggccggc
      661 tgcgtgctgg ccgcgcgcct gagcgaggac ccccacaccc aggtcaccct gctggaggcc
      721 ggcccaaccg accacagccc gctgatccac tgcccggccg gcatcgccct catggcccgc
      781 accgggcagg tcaactgggg tttcgagacc gtgccgcagg cggcgctgaa cggccgccgc
      841 ggctaccagc cgcgcggcaa ggtgctgggc ggctccagct cggtcaacgc catgatctac
      901 acgcgcggcc accccagcga ctacgacgcc tgggccgccg ccggcaacgc cggctggggc
      961 tgggacgagg tgctgccttt cttcaagctg gccgaacaca acgaacgcgg cgccgacgct
     1021 tttcacggcg cgcggggccc gctgaacgtc aaggacctgc ccgagccgaa cccgttctcg
     1081 ctggccttcg tcgaggccgg ggtgcaggcc ggctaccccc gcaacccgga tttcaacggc
     1141 atcgagcagg agggcgtggg cctgtaccag gtcacgcacc gcaacggcga gcgctgctcg
     1201 gtggccaagg cctacctgac gccgcacctc gggcgcccga acctgcacgt gatcactggc
     1261 gcgcacgcca cgcgcgtgct gatcgagggc ggccgtgcgg tcggggtggc ctatgtgcgc
     1321 gacgggcgcg aggaacaggt gcgcgccgag cgcgaggtgc tgctgtgcgc cggcgcgctg
     1381 cagtcgccgc agctgctgtt gctgtcgggc atcggcccgg gcgagcacct gcgccagcac
     1441 ggcatcgcgg tgcgccacga gttgcccggc gtgggcgggc aactgcacga ccaccccgac
     1501 gtggtgcagg tctgggacgt gccgggcgcg cgcgagctgt tcggcctgtc gccggccggc
     1561 gcctggcgcg cggcgcgcgg cttggggcaa tggcggcgcg agcgcaccgg catgctgacc
     1621 accaacttcg ccgagggcgg cgccttcctc aagagcgatc cgggcctgtc cgcgcccgat
     1681 ctgcaattgc acttcgtggt cggcatgctg gtcaaccacg gccgcagcac ggtctggggc
     1741 cacggttttt cctgccacgt gtgcgtgctg cggcccaaga gccggggcag cgtgcgcctg
     1801 gccagcgccg acccgctggc cgcgccggcc atcgacccgg ccctgctgtc cgacccggaa
     1861 gacatgcgcc tgatggtgcg gggctttcgc cagatgcgcg agctgctcgg ccacccggcg
     1921 ctggcgcagc acggcgggcg cgaacgctcg gccgcggtgc gcagcgacgc cgagatcgag
     1981 gccttcatcc gccgctacgc cgacgccatc taccacccgg tgggcagctg ccgcatgggc
     2041 agcggcgcgc aggacgtggt ggacgcgcag ctgcgcgtgc acggcctggc cggactgcgc
     2101 gtggtggacg cctcgatcat gcccagcgtg gtggccggca acaccaacgc accggtggtg
     2161 atgatcgccg agaaggcggc gcagatgctg cgccagggcc aggcggcgca ggcgcgcgag
     2221 gcggtgctgg aagacgtcgc ctgatactaa ggatttatcc gtaaattaaa ctattgcctt
     2281 gagtcgcctg tgatgcgacg caggagcaat agatgggcaa ggcgcatgcc aattcgtggg
     2341 aagtaacgga cgagttctgg aagcgagtgg agcccttggt gccgcagcca gcacgcgatc
     2401 cccagaggca gtacaaacgt gcagccgggg caggccgccc gacgaagccg ccacggctgg
     2461 tgttcgaggc gatcatgtat gtgctgcgca ctggctgcca gtggaaggcg ctgccggctg
     2521 agcggtttgg tagcgccagc gcgattcatc agaagttcat ggtgtggtcc aaggccgggt
     2581 tcttcgagag tttgtggagc gccggtcttg ccgagtacga cgattgcgaa ggcattgcct
     2641 ggcgttggca aagcatcgat ggagccatgt tcaaagcgcc gttggcgcaa gaggctgtgg
     2701 ggcgcaaccc gacggatcgg ggaaaaaaag ggcagcaagc gtcatctgct ggtggacggg
     2761 cgtggcgtcc cgttgtcgct cgtcgtgacg ggggccaacg cgcacgacgt gacacagctt
     2821 gaggctgtgt tgtcgttttg catgatcaag cgtcccacac cccagcagcg ccgcagcaag
     2881 cacctgtgcg ctgatgctgg ttatcgggga aagaacgcca tgaagatcat gctggcccat
     2941 ggctacatcc cccatgtggt gggccgcaag agtgaggctg aatgcaagaa acgcgatccg
     3001 aagaagctgg cgcgccgctg ggtggtagag gcctgccacg ggtggttcaa ccggtttcgc
     3061 aagctgttgg tgcgctacga gaagcttgaa catacctttc tcgctctcaa ccatcttgcc
     3121 gctgccatca tcgccctgcg aaagatcagc ctgcccatca atattattta cggataaatc
     3181 ccaaaaggcg ttcaaaagca tcaaaccgtt cgcgttgagc ccttcgacgg gctcaggaca
     3241 ggcttgtcga aacgccgcgc cgggcttcga caagctcagc ctgaacggtt cttgcagtgt
     3301 gaacgccaat cggtacgagc aggtgccccc cggcgcgcca cccctggccc ccgtgctcgg
     3361 cgcaggacgc gggcaacctg gatttgctac tgttgttata gctactcagg atgaatgggc
     3421 gccgaccaac ggccaaaaaa ggcttgaaat ctggcacccg gacacgccgg ctcacttgta
     3481 gccgtgccgg tccatcatcc ggtaggcggt gcgctcggac acgcccagcg cgtcggccac
     3541 cttgcgccgg ttgccgtcgt actttttcag caggcgcgcc aggtattcgc gttcgatgtg
     3601 ctccagcgtg ggctcgccct gcagcaccag cgactcatcc tcggccgtgg cgggggcggg
     3661 cacggccatg gcgggggccg gcgccacggg cgcgggcagg ctgcgcaagc ccaggtgcgc
     3721 cggggcgatg cgcggcgcgt cgccgaccag gatcagggcg cgctcgacca cgttgcgcag
     3781 ctcgcgcacg ttgcccggcc agtcgtagcg ctccaaggcc tgccgcgcct cggcgcccag
     3841 cgccaacggc ggcaggccct tgccgcgccg gcgctggacg atgaagtgct cggccagggc
     3901 ggcgatgtcc tcgcgccgct ggcgcagcgg cggcacctcg atcacgaaag ccgccaggcg
     3961 gtagtacagg tcgtcgcgaa acagctgctg gcgcgcgcgc tgggccaggt cgcggttggt
     4021 ggcggcgacc acgcgcgcgt cggtgcgcag gtcggtggtg gcgccgacac gccgaaagcg
     4081 gccggattcc agcacccgca gcagcttggc ctggatcgcc ggcccggcct cgccgatctc
     4141 gtccaggaac agcgtgccct gggcggcggc ctcgatcagc ccggtcttgc gccggtcggc
     4201 gccggtgaag gcgccgcgct cgtggccgaa caactcggat tcgaacagcg tttcctgcaa
     4261 ggtggtgcag tccacggcca cgaagcgttc gtcgcggcgc gtgctggcgg cgtgcagggc
     4321 ctgcgccacc agctccttgc cgcagccgct ctcgccctgg atcagcaccg tgacgtcgct
     4381 gccgcccacc tcctgaataa gctggcgcaa gcggcgcatg gccgggctgt cgccgatcat
     4441 ggcgccgggt ccgtcgccgc aggcggcctt ttgcgccagc agctggcgcc gctccagcgc
     4501 gccgcggatg accagttcca gctcgtccag gttcaccggc ttgaccaggt agtcggccgc
     4561 gcccaggcgc atggcctgca ccgcctggtt cacgtcgccg taggcggtca gcatgaccac
     4621 cgagcgcgaa tgggcggtct cgcttaaggg gccgaagccg gtggagtcgg gcaggttcac
     4681 gtccagcagc accagatcgg gcgcgaagac ggcaatgcgc tcgcgcgcct gctgccacga
     4741 cgcggcgctg tccacgtcgt aaccgcactt gcccagcgtc ttgaccagca agcggttgag
     4801 gacggggtcg tcttcgacga ccagcagcga ggagctcatg gcacgggccg agaggagagg
     4861 tctggccccg gattgtcggg cagcgcggcc ggcgcgcgcc gcagcgtcag cgtgaaggtg
     4921 ctgccctggc cgggcgggct gctgacttcg atgccgccgc caaagcgttc catggtggcc
     4981 ttgcagatgg ccaggcccag gccggtgccg cgctggccgt cgacgcggcg gctgaaaaag
     5041 ggcatgaaga tcaggggcag gtcttgcggc gcgatgccgc agccgttgtc gcgcaccgcc
     5101 aggcgcaggc tggcgccggc ctcggccggc tcggccagga tctccacctg cccgccctgg
     5161 ggcatggcgt gcagcgcgtt gtggatcagg ttgaccagca cctggcgcag ctcggcctcg
     5221 tcgcccagca cctgctcctc gggcgcaatg ccgccgagtt gcagcgtgac gcggcgcgcg
     5281 cgaaactcct cgcccagcag cgccaacacg tcgccgaggg cggtttccag ccgcaccggg
     5341 cgcagctctt gcgacggcgc ctggctcatc tgcagcaggc gctgggtggt gagcacgcag
     5401 cggtcgatct cggtgtcgac cagccgcagg tagtcgatca gctcctcgct gggcatgtcg
     5461 ccgtcgcgca ggccgcgcag gcaggcctgc agggccaggc ggatcgaggc cagcgggttg
     5521 tggatctcgt gcgccacgcc gttggccagc aggccgatgg tggacaggcg ctgctgctgc
     5581 gagaagcgga tgctgcggtc cagcgggcgc atcacctcca ccgtgtagcg ctcgccgccg
     5641 ccggtggtga agggcgccgc ctcgatgtcc acctccaccg cgccgccgtc ggcgcggcgc
     5701 agcgtcatca ggctgtgcac gggctcgggc cgcagccgca tctcggccac cgggcaggtc
     5761 agcaaggtgc tgggacaggg ttcgttcagg ccgcggccga cacggtggca gcactggccg
     5821 atgacggccg gcatctcgtg tccgagcaag cgcgcgtagg cctggttggc caggcggatg
     5881 cgaaagtccg gcccgatcac cagcaccggg tcgggtaggg cgtcgatcag ggtctgcaga
     5941 aagcgccgct cctcgcgcag cgcgcccacg gtgtggtcga tgcgctcggc catgcggtcg
     6001 aagctgcggc tgacccggcc cagctcgtcc ggcgcgcgcg agtcgacgcg ggcggccagt
     6061 tcaccgccgg ccaggcggtc ggcggcccgc gccagctggc ccagcggccg gcccacgcgg
     6121 cggcgcagcg tcaggctcat cagagccgcg aacagcccca gcgacaacag ccccacggcc
     6181 agcagcagcc aggccggacc ctggcccagg ccggtggtgg ccacgggcgc gaagtcgagc
     6241 agcagcaccc cgttcaccgg gtgctgggcc ggcgcgccgt ggcagccggc gcagcgcgcc
     6301 tggttcagca ccgggtaggc gatgtggagg cgctcgctgg cttcgccggg gtaccacgtc
     6361 aggcgcggcg ttggcggggc gcagccggcg gtcaggcaca gcccggccaa ctgctcgccg
     6421 gccgagccgc cgaccgcctg tggccgcgag gcaaagcgca ccgtgccccg cggttcggcc
     6481 aggcgcgcct gcgtcacgcc cggcacctgg cccagcgccg acaggatggt cgacaggccg
     6541 tccaggtcgc gcttgagcat ggcgttgtgc agactggcct cgaacagatg cgccatgcgc
     6601 tgggcggcgg cttcgtgctc ggcgcgcacc gcgccccggc tgacggccag cgccagcgcg
     6661 gtcagcaggc cggccgacag cagcagggcc accaccaggc ccagcgtgag ctgacgcgac
     6721 agggacgagg cgatccaggc gcgcaggccg ccgccgcggc cgggtggcgt gtcggccgag
     6781 ggcggggtgt cgggagcggt ggaggcggtc atggcgggca gtcctgctgc accagccgat
     6841 tgtgctgccg cggccggcct tgccgacagg tcgggcagca cggcgtgggt tcgcgctggg
     6901 cgccggccgg ctgatgaaag tccacccggc aggccgggca agacaagcgt gtcaggagca
     6961 cttcgtctgg acctggcgcg gcctcgtgct cggtcggcgc gcgcggggtg gacagcgcct
     7021 gtggaatgga cagcgcctgt ggcgtgcaca cgtcgtggca caggccacag ccggtgcagc
     7081 ggcgcatgtc gagcacgaag cggtccggcg cgcctggcgc cgtggcgcgc tcaagcgcca
     7141 gcgcgccgct cgggcagacg cgcgtgcagg ccaggcacca ggtgcagcgc gcgctgtcca
     7201 gcgccaccgc ccagagcggc gcggcgcggg ggctggcggc gctcaactgg gcggtgatcc
     7261 aggcgcgctg gccggccagg ggcggcagcg cggccagcgc cgcgccgacc ggtgcggcgc
     7321 gggcgcccag aaagcggcgc cgccccggcc gtggcgcggc ggcgtcggcg ggttcgggcg
     7381 ccggccaggc ggtggcggcg atcgggctca gcgccggcac gcggcggccc gcggcgcgca
     7441 ggcgctcggc cacggccagc cagtgcgcgc gccagcccgg gccggcgccg ccgcgcgggc
     7501 agtgggcgca gtcgcccggc gccagggcca tggtgtcggc cgacagcgcc tcggcggcgt
     7561 gcagcagcca gcccggcgac aggccgtaca ggcagggtgt gacgccgggc gcaccggcca
     7621 cgccagcccg ttcgcaggcc agcagcaggg tccgcgcgcc gcccgggcgc gtgaccacgc
     7681 gcggcgcggg ggccggcagc gccagcgcat cggtggggca ggccgccacg cacaggccgc
     7741 agtcgtcgca ggcctcggcg tcgaagccca ggccgtccgg gtgcgcgcgc caagcctggc
     7801 gcgggcaggc cgtcacgcag gcctggcagc cggcgtgcag cacctgggcg tgcacgcagc
     7861 gctcggcctg gatggcaagc cggctggctc cctgcgccgg atcggccgcc gcctgtggcg
     7921 cgggcatggc aggcgcgggg cctcaggaca aggcgccgca accccccggc gggaccgccg
     7981 cgctctgcgc caccacgggc ggcagcacgg ccacgtcggg cagcgcctgc tgacaggctt
     8041 cgcaggccga cagggtcagc gcggccaggg cggcgtagaa cggcgtgtcg gcgcgttgca
     8101 ccacccgacc ggcgaactgc ggcagccagc tcatcaggtg ggtcttgaga aagcgcgccg
     8161 cctcgcgcgc ctgctcgcgc tccagcagca gggccacgaa ctccagctcg tgggtcaggt
     8221 ggtcgtccgc gcgctggcgc cagtcgggca cctgcacgcc atggcggcgg tagaactcgc
     8281 gcacgctgaa ggtgggcgct tgcagcatca ggtggtcgtc gtcgcgccac accgactcgt
     8341 gcggcgaggc gcgcagcgtg tgggtgaggt agatcgccgc gtagtcggcg gccaactgat
     8401 cggcatcggt gtacggcaat tcgccctcgc tgtcgcagcg gctgctggcc aaggcgcgca
     8461 gcgcgacggc catggcccgc gactcggggg cgtcggcatc gaccagcacc agggtgtcgg
     8521 gaaagccgtt ggcgtacagc tcggccagca gctcgggcgc gtgttccttg gcgtgcagcc
     8581 aggccaaggt gcgcaagtcc tcggcggcca gggccggcca ggcggggcga gaggcgggca
     8641 cggcgttcat gacgaggcct tgggggtggg cgcggccggc acggcggggg cggactcgac
     8701 cttgcccttg gggtgcgcga cgaacacgat ggaggggttg gtgagttccg ggttggccat
     8761 gtgctggact tgccgcaccg gcacgtccac gcccttgacc atggccttgg tgtcccaggt
     8821 gccggcgcgc agctggtcga tggggccgat gtcgagcacg cgcatcatgc aggccgacac
     8881 gcagtagggt ttgcggccag actccagctc gtcgatgcac aggttgcact tcttgaccgt
     8941 gttgtcgctg gggtcgaact gcggcgcgcc gtaggggcag gcggcctcgc agcgacggca
     9001 accgatgcac agcgaactgt cgatgtccac cacgccgttg tcggcgcgct tccagatcgc
     9061 gcccgtgggg caggtcggca ggcaggccgg ctcggcgcag tggttgcagg acatgttgac
     9121 cttgtaggcg aacacttccg gaaacgtgcc gccttcgacg tacatgacgc ggcgaaagcg
     9181 tgggccgggc ggcagaccgt tcttgtcctt gcaggcgatc tcgcaggcac gacagccgat
     9241 gcagtccatg ttgttgtgga tgaaacccca ctgctgctcg ggctggaccg gctcgtaggt
     9301 ggtggcgtcg cgccggtcct gggcgtactt gacgcgcgcc ttggcgtcga tgcccagctt
     9361 gttgatgact tgtttctcgg ccatggcggc ggctccttac atgtcgcggc ggaacaccgc
     9421 cgaacgtgcc acggccagct cgtcccagcc gggccggtgc gtcaggttcg acttctggat
     9481 ctgcaccatg gcgttgttgt aggcaaaggc gccggccgga ctggggttgt ccagggtcag
     9541 gaagtcgggg ttgccggccc ggtccacgcc gttcttgtcg gggtccatcc aggcgccctc
     9601 gaacagcacc aatacgccgg gcagcaggcg ctcggtgacg taggcgggca ccacgcactt
     9661 gccgcgttcg ttccacacct cgaccgtgtc gccggtctgg atgcccagct ttctcgcgtc
     9721 gctggcgttg atcgtcacct cctgctcgta ggtctcgcgc agccagggaa tattgttgaa
     9781 gatcgagtgc gtgcgccagc gcgggtgcgg gctgatcagg tggaacgggt actgcttggc
     9841 gatcgggtgg ttgagcgact caaagggctc gatccacttc gggatgtacg gaatctcgta
     9901 gccgtactgc gtcttggtcc agtcggtgat gccggccagc tgggtggaga agatctcgat
     9961 cttgccggag ggcgtttcaa acggcttgcc ctgggctatc tgcgcgcgga aggccacgtg
    10021 cggctccttg agcacgaact tgtaggtgcc gtgcttcttg aactcttccc aggacatctt
    10081 cacgccctgg tggtgctgca cgctgccctc ccaccaggcc ttcaggtagg cctcgtcgac
    10141 cgcgtccggg tgctggaagt agtcgcggtc ggcgcgtggg ttgtagcgct tgcccaggtt
    10201 ctccagcccg ggatcgagcg cttgcaggcg gtagctcagc tcggtgaaga cctggaagtc
    10261 ggtcttgctt tcacccagcg gctcgatgac cttgggccga tggatgtagt agtggccctt
    10321 gtaccagggc aaggcgacgt cgtggcgctc gaagtgcgtg gcgatcggca gcaggtagtc
    10381 ggcccagatg cccgagggcg tgatggtcga atccatgcac accaccaact ccagcttttc
    10441 gatggcctgg atttccttgt tgatgttggt gagctggttg aaccagtcgc tgccttgcca
    10501 gaagatgccc ttgatgtggg gaatcttgcc gtccagctcg tcctcgcgcg gccacagacc
    10561 gatttcctcg cgtttcaggt tcgggtagtt cagcacgcag tgcgcccagc ggtcggactt
    10621 gatcgaggcc caccacaggt tggcgttctc gtcgtacggg taggccacgc tttcgctgtg
    10681 ccaggccttg cccacgccct cggcgcagcc gcccagcacg ccgatgttgc cggtcatggc
    10741 ctgcagcgcc gcggccatgc ggttgtactg ctcgccgtag ctggcgcggc cgggcgccca
    10801 gctggccttc agcgcggccg gcttggtctg ggcgtacatg tcggccagct tgcggatgtc
    10861 ggccgccttg acgccgcaga tgggctcggc ccactcgggc gtcttgggcg tgttgtcgta
    10921 cttgccgagg atgtagtcct tgaagttctg cttgccctcg aactgctggc ccatggtgcc
    10981 cgcatccatg cccagcacga acttgtcgat gaatttctgg tcgtgcaggt tggcggtgaa
    11041 gatgtggtgc gccatggcgg ccatcatggc cgcgtcggtg ttgggcttga tcggaatcca
    11101 ccaggcgtcg tagacggcgg ccgaatcggt gtactgcggg tccaccagca cgaacttgca
    11161 gccgcgctgc ttggccatgc gcatgtagta gaaggtgttg ccgccgtgga aggtgtaggc
    11221 cgggttccag ccccacatga tgatcagctt gctgtgcgcg aaggtgtcgt cctcgttgcc
    11281 gtcctggatg gtgccgaagg tcacgcggct ggagaacgtg gtggcctggt aggagggcac
    11341 cgaccaggaa ttggtgcggc agccgaacat ccccatgaag cgcccgagca gcccttcgat
    11401 ctggtcgctc ttgtgcagca cgccatagct ggcgccggcg tagctctggt ccagcaaggc
    11461 cgtcgggccg tagtccttct tcaactgcac catcttctgg gcgatttcgt ccagggcctg
    11521 gtcccagctg atgcgcttga acttgtgttc gccgcgcgcg cccacgcgct tcatggggta
    11581 gagcaggcgt tcgggcgagt acaggcgcga acggtagctg cgcccgcgca ggcaggcgcg
    11641 cagctgcggc tcctcgaaag tgtccttgcc gtaggcgccc tgggcctggt attcgccgtt
    11701 gtcggtcgac aggcgcacga tcacgtcgcc ctttttgtgc gccaccagca catggcgcga
    11761 gccgcagttg tgcgcgcagg aggtgaccac cgtcgtcagc tggtcgtcgg tgaagtaagg
    11821 ctcgtaggcg aaggcctcga ctttttcggg cgccaccgtg acccccagcg tcaccaggcc
    11881 ggcggcgccc gcgccggcgc cgacgacctt gagaaaatcg cgccgccggt agcccaggcc
    11941 cacctggtcc atggattcac cgacgctggg aaagcgcgtg cccggccctt gagacaaatc
    12001 gttcttcatt tggacgctcc ctgcgcggcg gcggtggtgg ctggctggac ggcggcgctg
    12061 atgaagcgca cttcgctctc ggtcggccgg cccttggccc aatcgggcac ctcggtcggc
    12121 atgccgggcc aggcgtggcg cgggtagggg tagtgcgtgc ggtaccaggc gcgaccaccg
    12181 tggcagccaa agcacacgtc gccgttggtc ttgccggctt cgtcgatggc ctgggcgttg
    12241 aggtagttga cctggtgccg catggtgcgg atggcggcgt ggcacagcat gcagttgttc
    12301 tggaaactgt ccttggactg cgcccagggg ctgggcaggc ccatgacctc ccagttcatc
    12361 ttgccgtggc atttcaggca gctggtctcg gggttgatca tcttgcgcag cgtcagctcg
    12421 gcgttctggt tgctggccga ggtgccgggc gcctcgtcgc gcgggtcgtt gccctggtgg
    12481 caggtggtgc agcgcatgtt catcagctgc ttggccatgg cggtgtcgag atggcggcgg
    12541 tgaaaagtgt cctgctcgcc ctggtaggtg gaggtgacct ggtaccaggc cttggcggtg
    12601 gcggcctcga cgccggccgg cgaactcttg cgcaccgagg gcttcagcac ttcggcgtga
    12661 cagctcaggc attgcgcgtc ggtggccttg tcgatggccg gcttgaagtg gatcgggtcc
    12721 cacttggcgg cgtggtaatc ggtgggctgg gccggtgcgg cggtgggcga cgcggacgcg
    12781 ccagccatgg gttgcgcgac agcgggcgcg gggggctgcg gatccgagca gccgacgaac
    12841 cagagggcgg ccatcagaac gatcgaccac gacagaatgg gcctcaagct gggcgctttc
    12901 atgaccttgc tccgaccaag ggaagggggc gagaaaaatg ccgggctggc gcgcgggcca
    12961 gcccggccct aaagccgccg cccagccggg cggcggcgtg tcggcggctg gattacttct
    13021 tgtcgccgct catgcccgac atgccagaca tgccttgctg acccgacatg ccgctcatgc
    13081 cttgctggcc ggacatgccg gacatcccgg acatgccctg ttgacccgac ataccggaca
    13141 tgccggacat gccttgctgg gccggcttct tcttggccgg ggccttgttg ccgctcatgc
    13201 cggacatgcc gctcatgcct tgctggccgg acatgccgga catgccgctc atgcccttct
    13261 ggccgctcat gcccgacatg ccttgctggc ccgacatacc gctcatgccc tgttgaccgg
    13321 acatgcccga catgccactc atgccctgct ggccggacat gccgctcatg ccttgctgac
    13381 ccgacatgcc ggacatgcct tgctgctgag ccagcgccgg aagggccgcg accagtgccg
    13441 ctgcgaccac cgacagaccg agaacgtgac gaactgcttt catggaactc tcctttgtta
    13501 ccgataaaaa aggtctattg cgcaagattc ggggccgaag agccccctgc ctgtcggcag
    13561 catcgccgct gtcgccgcgg acggcggcaa gcgcgatcgg gtggcgcgcc ccactcgcca
    13621 gggcgagaca ggtcgcgtgg aaaacggaag ccggccatgg ccaggttcag cgcgcggccg
    13681 aggcgggcgc ggccagggtg gccggctggg ccgcgccgta caggccgcgt gggtcgtagg
    13741 gggcgggcat gccgggatgg cgcatcggca cccaccaggg gccggcggag acgatggcgc
    13801 gcaggttgcc cggcggcacg ccttccacgc cgcgcagcgc ctgggcggcc tgcgcgtcgg
    13861 tcctggtgaa ctgggccacg cgtggcgcgc cgtccggccg gcggtcggga tgaaggccgg
    13921 cgatggggcc cgaggtctgg gcccagctgg cggcgcccac cagggcgagc agcaaggcaa
    13981 tcaagggtgt cgaggtcacg gcgagtcgat ggcagtggcg aaatgcctgt catcaagcaa
    14041 aaggcttgcc agccgactcg atttttttct aagtgactat caacaaaagg tttttttcta
    14101 ggcgacgggg tgattcgacc cagctctgcc agagtggcag gccggcgccg cagtcggcct
    14161 gccaaaacgt catgtcaaag tggcgaggcg ccagcccgca gatgtttcac gcctcgattt
    14221 ttgggctaaa accggccttg gtcggcgccc atgcatcctg agtagctatt aaaaaatgag
    14281 tactctcagc gccgaaagcg ctgccgggtg aaggccaggg cgacccagaa gccgacgatg
    14341 gcgtaggccg ccagcaccgc caggtggcgc cccgcgttgg ccggccattg gtccaggaac
    14401 agcggccgca ccaggtccac caccgccgac agcggcagcc agttggcgat ggcgtgcgcg
    14461 aacggcggca gttgctcgcg cgggaagaac acgcccgaca agaacatcat cggcgacagc
    14521 agcaccgcga agtagtaggt gaaaaagtcg tagcccttgg ccttggcgtt gaacaccagc
    14581 gccatgctgg cggcggtgat gccggccagc agcaggatgg gcagcaccag cagcagcttg
    14641 gggctgtggc tgatttgcag cgccagcatc acgcccagga tgccggtggc cagcaggacg
    14701 ggcagcgcca gcagggtgcg cggcgcgcgg ctg
//
LOCUS       DAOOOG010000043        14622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4405_length_14622_cov_25.143956, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000043 DAOOOG010000000
VERSION     DAOOOG010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14622
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4405_length_14622_cov_25.143956"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..851
                     /locus_tag="PK242_05170"
     CDS             237..851
                     /locus_tag="PK242_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HOZ93440.1"
                     /translation="MRSLLLHRRAKPLVFVLCLLPLAWLSYGAAADQLGANPAEALIR
                     GLGDWALRFLCLALAVTPLRIRTRTPQLARFRRLLGLFTFFYACLHLLAYGWLDMGLD
                     PGDIGRDILKRPFILVGFASWLLLLPLALTSTHRAVRALGGRQWQALHRLVYVVAPLV
                     LLHFFWMRAGKNDFAEVAVYALILGALLLERLLRYFEPKSRLSA"
     gene            complement(933..2228)
                     /gene="lysA"
                     /locus_tag="PK242_05175"
     CDS             complement(933..2228)
                     /gene="lysA"
                     /locus_tag="PK242_05175"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOZ93441.1"
                     /translation="MTSPATTTPALLPGGAALSFQNGQLMLEQVALSALAREHGTPLF
                     VYSRAAILSALAAYQRGFAGRRAQICYAMKANASLAILRLLAEAGCGFDIVSVGELER
                     ALAAGATPSKIVFSGIGKTRQEMRRALEVGIGCFNVESLPELDVLSEVAQSCGQRAPV
                     SIRVNPNVDARTHPYISTGLKGNKFGIAHEQALAAYRHAASLPGLRVAGIDCHIGSQI
                     TEVAPYLEAMDRVLDLVTAIEAAGIAIGHIDFGGGLGIDYRGETPPAADALWAELLAR
                     MDARGFGERQIMIEPGRSLVGNAGVCLTEVLYLKPGEQKNFCIVDAAMNDLPRPALYE
                     AYHRIVPVVQATPDAATSTWDVVGPVCESGDWLGRERALAVRPGDHLAVLSAGAYCMS
                     MASNYNARPRAAEVLVDGAQARLIRRREQLQDLWRDELG"
     gene            complement(2225..2473)
                     /locus_tag="PK242_05180"
     CDS             complement(2225..2473)
                     /locus_tag="PK242_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93442.1"
                     /translation="MSFAQPRRFILVSGMALAVAALAGGCGQKGALYLPDSPAAAQRA
                     SLPQTVFGGAKSTAAPAPAAAPSRSPPPPVLPDLPDIQ"
     gene            2487..2819
                     /gene="cyaY"
                     /locus_tag="PK242_05185"
     CDS             2487..2819
                     /gene="cyaY"
                     /locus_tag="PK242_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HOZ93443.1"
                     /translation="MTDLEYLDRAETLLRQVEAQCDHLNDHSDADIDNQRSGGMLTLV
                     FPDRSQIVVNLQKPLHEVWLAARGGGYHFRFVDGAWRDTKSGQEFFRQLSQSASEHAG
                     LALRFAPD"
     gene            complement(2862..5240)
                     /locus_tag="PK242_05190"
     CDS             complement(2862..5240)
                     /locus_tag="PK242_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HOZ93444.1"
                     /translation="MNKRTDPSKGGWQRWLLRALAWTAGAALAGVLSVLLGGLMALAI
                     AYPNLPDVSELSNYRPKLPLRVYTADGTLMREFGEERRNLTPIGEIPQVMKHAVLAIE
                     DARFYEHSGVDYLGVARAALANLGREKSQGASTITMQVARNVYLSSEKTYTRKIYEVL
                     LTFKLEHLLSKDQILEIYMNQIFLGNRAYGFAAASETYFGKPLKDVSIAEAAMLAGIP
                     KFPSTANPIANFTRARDRQLHIIDRMQDNGFITAEQAAAAKQQELRIRPVNEASRVHA
                     EYVAEMVRQMMFAQYGDDTYSRGLNVYTSIRAADQNAAYTALRAGILDYDRRQAYRGP
                     ERFIELPGNPKELDEAVDDALASHPDAGELLAAVVTRVDAQGRSASVMRRGGETVEIA
                     ADGLRAVASGLSAKAGPNIRIRPGAVVRIVRNSRNQWAMTQIPEVEGALVALDPRNGA
                     IKALVGGFDFDKNKFNHVTQAWRQPGSSFKPFIYSAALEKGFSPTTMINDTPLFFDAS
                     VTGGQPWEPKNYEGGFEGPMTMKRALARSRNIVSIRILQTITPRYGQEWITRFGFDAD
                     KHPPYLTMALGAGSVTPIQLASGFAVFANGGYRISPFLITRVTDHTGKVLVESQAEPP
                     SESSRAIDARNAFIMNSMLQEVARSGTAAKAQATLKRPDIGGKTGTTNDSMDAWFTGY
                     QPTLVTTVWVGYDTPRNLGSRETGGGLSLPIWINFMQTALKGVPVADAKPPEGVVQVG
                     GDWYYDEYARGGGISNLGGAGLEAADPAAGQEAGAGAASSGERNRILDLFRN"
     gene            5580..6662
                     /locus_tag="PK242_05195"
     CDS             5580..6662
                     /locus_tag="PK242_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOZ93445.1"
                     /translation="MVLKTGSLFNRPPAPLIGLDVSTSSVKLVELGQDRSGQLTLERC
                     GIELLESGWISDGNIEKFDEVVEATRRLISRSGTKTKNVAMALPASVVISKKIILPGG
                     LSEREMEVQVESEANQYIPFSLDEVALDFSVVGPSATSAGDVEVVIAASRKEKVEDRQ
                     GLAEAAGLVPVVMDVETYAARLAVSRIIGRLPGGGVDAVIALFEVGGLTSTLQVLRND
                     DLVYERDQVFGGAQLTQMLVRQYGFTFDEAETKKRAGELPDDFGSVVLQPFVESLAQE
                     IERALKFFFTSTPHHRVDYVLLAGGTASLPGLTEVVTRLTSFPCQVVNPFDGMDVART
                     VREKRMQREAPSYLTACGLAMRRFLR"
     gene            6659..7267
                     /locus_tag="PK242_05200"
     CDS             6659..7267
                     /locus_tag="PK242_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOZ93446.1"
                     /translation="MILINLLPHREMARKKARQVFNTSLVGSVLLGALVAGGVYLWLQ
                     NQISNQQERNGFLTSEIKKLDEEIKEVATLQDEIAALKARQQAVEDLQANRNLPVHLL
                     NESVLQLPDGIYLKGIKQDGQNVLLTGVAQSNERVSELLRNLSRHSDWVSKPELIEIV
                     ASNLALSAREQRRVYNFTMRVQLQRASAAQAAASAAPRAKSS"
     gene            7281..7964
                     /locus_tag="PK242_05205"
     CDS             7281..7964
                     /locus_tag="PK242_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOZ93447.1"
                     /translation="MASTSIRNIDVSQWLRQAGAQFSGLDRRDPSSWPAVPRYALFLC
                     IAALVVGLMWFLWLTDVEQELEAERNREATLRTEYQGKLQKAVSLEELKKQRAQVMQY
                     VTLLEKQLPSKAEMDALLSDINQAGLGRSLQFDLFRPGQLVVKDYYAELPIAVKVTGR
                     YHDIGAFTADIANLSRIVTLDNLSIAPSTDKDRAGALSLEATAKTYRYLDPEEIAAQR
                     EAAKPKAKK"
     gene            7961..8506
                     /locus_tag="PK242_05210"
     CDS             7961..8506
                     /locus_tag="PK242_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HOZ93448.1"
                     /translation="MSRAVLILLTAGAVLAGLAGCTANDEDLRQWMAEQRRTTTPKVT
                     PLTEPTRYMPLAYAGGAAVDAFSSERLTQVLRRDSGPLGGGIALLTSELNRRKEPLEA
                     YPLDAMTMVGSLDKGGQKVALLKVENLLHQVRKGNYLGQNYGRVTQITESEITLREIV
                     QDAAGEWIERGTTLQLQENTK"
     gene            8557..10662
                     /gene="pilQ"
                     /locus_tag="PK242_05215"
     CDS             8557..10662
                     /gene="pilQ"
                     /locus_tag="PK242_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HOZ93449.1"
                     /translation="MLLWGAAGLSLAEPRIQAVSSSIQGGTEVVKIDLSEPLAAVPAG
                     FLVQSPARIALDFPGVGSDVQRSMIELKHGNLRGAQVVQAADRTRVVLSLQQPANYRA
                     EVQGNSVLVSLDQVVTAPAAAAAPARTFAESGNVDALPLRDIDFRRGADSTGRVVVDL
                     ANNQVGVDIRQQGQNLVVEFLRTSLPEGLRRKLDVSDFGTPVQTITTVQSGDRVRMVI
                     EPRGLWEHSAYQSDNQLVVEVRQQKVSADKLTQGPGYRGEKLSLNFQNIDVRSLLQVI
                     ADFTNFNIVTSDTVQGNLTLRLKDVPWDQALDIILQSKSLGMKRHGNVLQIAPKDELA
                     AKEKAELEAQKAIRDLEPLRTQSFQLNYTKAEDIKQQLTSTSGGGGGTGGGSEGRLLS
                     SRGSVIAEPRTNQLFVSDIPSRLEQIQDMIAKLDVPVRQVLIEARIVEASDTFGKSLG
                     VKLGGADLRGIRGGDAGYGLGSSGNRVAFGGNYNAITSTTGQTDASGLTSLTSQFVNL
                     PASGLAGATPASFAISLFSSAANRFLNLEISALEADGKGRVVSSPRVVTADQTKALIE
                     QGTEFPYEQATSSGATSIAFRKAVLKLEVTPQITPEGNIILDLDVNKDSPGTEVRGGM
                     SINTKRVKTQVLVENGGTVVIGGIFELTERESENKVPVLGDVPVLGNLFKQRTRQAEK
                     QEMLVFITPKMISDRGAVR"
     gene            10715..12169
                     /locus_tag="PK242_05220"
     CDS             10715..12169
                     /locus_tag="PK242_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HOZ93450.1"
                     /translation="MRKLIGWVGLAAATLLTACGGGGGSPGDTPESYTITLRAAKTSL
                     PLNVAHYPAGIGVDAPFTTTLYVNAKKGDAPIPGGEDIFACNTSYGLDSGPLYYLDGD
                     DEHEDDDGNPLAYRSITLGSNAGGNSFHFHAGNQAGVATITCSVHDPRANREVSASVD
                     ITVGGGSGTGMAASIRGVAQHATLGTQGNLSNLRTSTAISAYVWDDANQPVPSNGKPN
                     VQASIVGSVAPGAPAWGARLLAGAQQGSTVQVPTTGGVGLFALSSGPNRGPILLELKA
                     DRRDNDVSNGIQDEVRHLLVVWAVEGVAATPLSLKPVEFDVPNATPFGAVLSVTGGEA
                     PYTWTALDALPTGLSLSSGGVLQGTPRVPPGTYVVRVRVTDALDNTAETSVTLKITGP
                     MTPPLVFTASAITAEVNLPFSYAFTASGGTPPYTWAALGPLPAGLSAPPSFGADGVIT
                     GTPTTPGAYTVAVRVTDSAGASVTQNVALTVDTP"
     gene            12270..12809
                     /locus_tag="PK242_05225"
     CDS             12270..12809
                     /locus_tag="PK242_05225"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOZ93451.1"
                     /translation="MHIFLVGMPGSGKSTVGRALARRLQLPFVDADTVIEERLGCSIG
                     DYFDHQGEAAFRDVEQGVIDELTQGPPGVLATGGGAVLRPANRQHLRERGTVIYLRTS
                     PEQLQRRLRHDTKRPLLQVADPLARLRALYAERDPLYRECAHYVLDGHGASQAMLVGR
                     IVMQLELSSPAPPDPAGGG"
     gene            12832..13941
                     /gene="aroB"
                     /locus_tag="PK242_05230"
     CDS             12832..13941
                     /gene="aroB"
                     /locus_tag="PK242_05230"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOZ93452.1"
                     /translation="MQASSSVSQVRIALGERTYPILIGAGLLADPGSYQAAPSGGDAL
                     IVTNTTVAPLYAERLARALAGRQRQVHQVALPDGEQHKHWETLNRIFDVLLGHGADRK
                     TVLYALGGGVVGDMTGFAAACYMRGVPFVQVPTTLLAQVDSSVGGKTAINHPLGKNMI
                     GAFYQPRLVLCDLDVLASLPARELSAGLAEVIKYGPIADMAFFDWIEANVDALRACDR
                     DALAHAVERSCQIKAEVVGQDERESGLRAILNFGHTFGHAIEAGLGYGAWLHGEAVGA
                     GMVMAAQLSHALGLVDAAFVQRLTALIARAGLPTRGAVLDAHDNAGRYLQLMRLDKKA
                     EGGEIRFVVIDGPGRAAVRAAPDALVRQVIERCCL"
     gene            14016..>14622
                     /gene="dgt"
                     /locus_tag="PK242_05235"
     CDS             14016..>14622
                     /gene="dgt"
                     /locus_tag="PK242_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HOZ93453.1"
                     /translation="MLRCASDPARSRGRRFAVTPAPTRNDFQRDRDRIVHSTAFRRLV
                     YKTQVFLNHEGDLFRTRLTHSLEVAQLARDIARALGLHEDLVEAIALAHDLGHTPFGH
                     AGQDALHECMAAHGGFEHNLQSLRVVDQLEQRYPHHDGLNLSFETREGILKHCSAAHA
                     RAIEAREPGGVARRFIDRTQPSLEAQLANLADEIAYNAHDID"
BASE COUNT         2361 a         4972 c         4975 g         2314 t
ORIGIN      
        1 gcacaccgat cacggcactg gcaggcgcat ccaactgccg tttctaggtt gaaccccgac
       61 agaccgcggt cgaccgcggg ctccccgcat gaaccccgca tgggcaccgc gtctcggtcc
      121 aggatcgatc ttccggcctt cgggtggggt gacgccgtgc tgccggatgc tataaacaac
      181 atagctgctg gcgattgtca gacaggcgcc gcagcccgat ttgacctata ttcctgatgc
      241 gttccctgtt gctgcatcgg cgggccaagc cgctggtttt cgtgctgtgc ctgctgccgc
      301 tggcctggct gagctatggc gccgccgccg accagctggg cgccaacccg gccgaggcgc
      361 tgatccgcgg gctgggcgac tgggcgctgc gctttctgtg tctggcgctg gcggtgacgc
      421 cgctgcgcat ccgcacccgt acgccgcagc tggcgcgctt tcggcgcctg ctggggctgt
      481 tcaccttctt ctacgcctgc ctgcacctgc tggcctacgg ctggctcgac atggggctgg
      541 atccgggcga catcgggcgc gacatcctca agcgcccctt catcctggtc ggcttcgcct
      601 cctggctgct gctgctgccg ctggcgctga cgtccaccca ccgcgccgtg cgtgccctgg
      661 gcgggcgcca gtggcaggca ctgcaccggc tggtgtacgt ggtggcaccg ctggtgctgc
      721 tgcacttctt ctggatgcgc gcgggcaaga acgacttcgc cgaagtggcg gtctacgccc
      781 tcatcctggg cgccctgctg ctggagcgcc tgctgcgcta tttcgagccc aaatcaaggc
      841 tcagcgcatg atgggcaagc gccggcagct ctcatattga tagtcaccgg aacggtgggc
      901 gacgcccgcc gggcggcgcc gccggcaggc gctcagccga gttcgtcgcg ccacaggtcc
      961 tgcaactgtt cgcggcggcg gatcaggcgc gcctgggcgc cgtcgaccag cacctcggcg
     1021 gcgcgcgggc gggcgttgta gttgctggcc atgctcatgc agtaggcgcc ggccgacagc
     1081 acggccagat ggtcgcccgg tcgcaccgcc agtgcgcgct cgcgccccag ccagtcgccg
     1141 ctttcacaca ccgggccgac cacgtcccag gtgctggtgg cggcatccgg ggtcgcctgc
     1201 accaccggca cgatgcggtg atacgcctcg tacagcgccg gccgcggcag gtcgttcatg
     1261 gcggcatcga cgatgcagaa gttcttctgc tcacccggct tgaggtacag cacctcggtc
     1321 aggcacacgc cggcgttgcc caccagcgag cggccgggct cgatcatgat ctggcgctcg
     1381 ccgaagccgc gcgcgtccat gcgcgccagc agctcggccc acagcgcgtc ggccgccggc
     1441 ggcgtttcgc cccggtagtc gatgcccagg ccgccgccga agtcgatgtg gccgatggcg
     1501 atgccggccg cctcgatggc cgtcaccagg tccagcacgc ggtccatggc ttccaggtag
     1561 ggtgccactt cggtgatctg cgagccgatg tggcagtcga tgccggccac gcgcaggccg
     1621 ggcaggctgg cggcgtggcg gtaggcggcc agcgcctgct cgtgcgcaat gccgaacttg
     1681 ttgcccttca ggccggtgga gatgtagggg tgggtgcgcg catccacgtt ggggttgacg
     1741 cggatgctga ccggcgcgcg ctggccgcag gactgggcca cttcgctcag cacgtccagc
     1801 tcgggcaggc tctcgacgtt gaagcagccg atgcccactt ccagcgcgcg gcgcatttcc
     1861 tggcgcgtct tgccgatgcc cgaaaacacg atcttcgagg gcgtggcgcc ggccgccagg
     1921 gcacgttcga gctcgcccac cgagacgatg tcgaagccgc agccggcctc ggccagcagg
     1981 cgcaggatgg ccagcgaggc gttggccttc atggcatagc agatctgcgc gcggcggccg
     2041 gcaaagccgc gctggtaggc ggccagcgcc gacaggatgg cggccctgga atagacgaac
     2101 agcggcgtgc cgtgctcgcg cgccagcgcg gacagcgcga cctgttcgag catcagctgg
     2161 ccgttctgga aggacagcgc ggcgccgccg ggcagcagcg ccggcgtggt ggtggcggga
     2221 gaggtcattg gatgtcgggt aagtccggca gaaccggggg tgggggggag cggctgggcg
     2281 ctgcggcagg cgccggcgcg gcggtggatt ttgcaccacc gaacaccgtc tgtggcaggc
     2341 tggcacgctg cgccgccgcc gggctgtcgg gcaggtacaa cgcccctttc tggccgcagc
     2401 cgccggccag ggccgccacg gccagggcca tgccgctgac tagaatgaac cgacgcggct
     2461 gtgcaaacga catggatgcc attgtaatga ccgacctcga atacctggac cgcgccgaga
     2521 ccctgctgcg ccaggtcgag gcgcagtgcg accacctcaa cgaccacagc gacgccgaca
     2581 tcgacaacca gcgcagcggc ggcatgctga ccctggtgtt tcccgaccgc agccagatcg
     2641 tcgtcaacct gcagaaaccc ctgcacgagg tgtggctggc ggcgcgcggg ggcggctacc
     2701 actttcgctt cgtcgacggt gcctggcgcg acacgaagtc aggacaggag ttctttcgcc
     2761 agttgtcgca aagcgccagc gaacacgccg gactggcgct gcgcttcgcg cccgactgac
     2821 cgcatcggaa ccacgagggc gccgggcgcc ctcacaggcg ctcagtttct gaacagatcg
     2881 aggatgcggt tgcgttcacc cgatgaggct gcgcccgcgc ccgcttcctg cccggctgcc
     2941 gggtcggccg cttccaggcc ggcaccgccc aggttgctga tgccgccacc gcgcgcgtac
     3001 tcgtcgtagt accagtcgcc gcccacctgc accacgccct cgggcggctt ggcgtcggcc
     3061 accggcaccc ccttgagcgc ggtctgcatg aaattgatcc agatcggcag cgacaggccg
     3121 ccgccggttt cgcggctgcc caggttgcgc ggcgtgtcgt agcctaccca caccgtggtc
     3181 accagggtcg gctggtagcc ggtgaaccag gcgtccatgg aatcgttggt ggtgccggtc
     3241 ttgccgccga tgtcgggccg cttcagggtc gcctgcgcct tggcggcggt gccgctgcgc
     3301 gccacctcct gcagcatgct gttcatgatg aaggcattgc gcgcgtcgat ggcgcggctg
     3361 gactcgctcg gcggttcggc ctggctttcc accagcacct tgcctgtgtg gtcggtgacg
     3421 cgggtgatca ggaacgggct gatgcggtag ccgccgttgg cgaacacggc aaaacccgag
     3481 gccagttgga ttggcgtcac cgaaccggcc cccagcgcca tggtcaggta gggcgggtgc
     3541 ttgtcggcgt cgaagccgaa gcgcgtgatc cattcctgcc cgtagcgcgg cgtgatggtc
     3601 tgcaggatgc ggatcgacac gatgttgcgc gagcgcgcca gggcacgctt catggtcatc
     3661 gggccctcga agccgccctc gtagttcttg ggctcccagg gctggccacc ggtcacgctg
     3721 gcgtcgaaga acagaggcgt gtcgttgatc atggtggtgg gcgaaaagcc cttttccagc
     3781 gccgccgaat agatgaaggg cttgaagctg gagcccggct ggcgccacgc ctgggtgacg
     3841 tggttgaact tgttcttgtc gaaatcgaac ccgcccacca gcgccttgat ggcgccgttg
     3901 cgcggatcca gcgccaccag ggcgccttcc acttcgggaa tctgggtcat ggcccactgg
     3961 ttgcggctgt tgcgcacgat gcgcaccacc gcgcccgggc ggatgcggat gttcggcccg
     4021 gccttggccg acagccccga agccaccgcg cgcaggccgt cggcggcgat ctccaccgtc
     4081 tcgccgccgc ggcgcatgac gctggcgctg cgcccctgcg catccacgcg cgtgaccacg
     4141 gcggccagca gctcgccggc gtcggggtgg ctggccagcg cgtcgtccac cgcctcgtcg
     4201 agctccttcg gattgccggg cagctcgatg aatctctccg ggccgcggta ggcctggcgg
     4261 cggtcgtagt ccaggatgcc ggcgcgcagc gcggtgtagg cggcgttctg gtcggcggcg
     4321 cggatcgagg tgtagacgtt caggccgcgg ctgtaggtgt cgtcgccgta ctgcgcgaac
     4381 atcatctggc gcaccatctc ggccacgtac tccgcgtgca cgcgactggc ctcattgacg
     4441 ggccggatgc gcagctcctg ctgcttggcg gccgccgcct gctcggccgt gatgaagccg
     4501 ttgtcctgca tgcggtcgat gatgtgcagc tgccggtcgc gcgcgcgcgt gaagttggcg
     4561 atcgggttgg cggtggacgg gaacttcggg atgccggcca gcatggcggc ttcggcgatg
     4621 ctgacgtcct tgagcggctt gccgaaatac gtctccgacg cggcggcgaa cccgtaggcg
     4681 cggttgccca ggaagatctg gttcatgtag atctccagga tctggtcctt gctcagcagg
     4741 tgttccagct tgaaggtcag cagcacctcg tagatcttgc gggtgtaggt tttctcggac
     4801 gacaggtaga cgttgcgcgc cacctgcatg gtgatggtgg aggcgccctg ggacttctcg
     4861 cgccccaggt tggccagggc ggcgcgcgcc acgcccaggt aatcgacccc gctgtgttca
     4921 taaaagcgcg cgtcctcgat cgccagcacg gcgtgcttca tgacctgcgg aatctcgccg
     4981 atcggggtca ggttgcggcg ttcttcgccg aactcacgca tcagggtgcc gtcggccgtg
     5041 tacacgcgca gcggcagctt gggccggtag ttcgacagct cggacacgtc cggcaggttc
     5101 gggtaggcga tggccagcgc catcagcccc cccagcagca ccgacagcac cccggccagc
     5161 gcggccccgg cggtccatgc cagggcgcgc agcaaccagc gctgccagcc ccccttggac
     5221 gggtctgtgc gcttgttcac ggcgtggcgc gaattctcag tggacatgga gcaatcagcg
     5281 gagttagtgg gtactttcat gaaacggtct gcgcggtcga gggcctgctg accgcagcct
     5341 gccgaacgcg cggaccggac ccgccattgt cagccgagcc gtgcgacgcg gccgttcgca
     5401 ggcccgccga tgtgtgtgaa gaccgcgaat ggggtaagga actgtcggca tccgttagtt
     5461 tttgacaacg accgtgtctg gtgttcgcag gctttttctt tgctggacaa aaggcttact
     5521 gctagcattc aagtgaattc ttaagtgtgg tcgacttttg ttaacaaaag taacaggata
     5581 ttgtcttgaa gactggttcc ttgttcaacc gcccgccggc gcccctgatc gggctcgatg
     5641 tcagcacgtc cagcgtcaag ctggtcgagt tggggcagga tcgttccggt caactgacgc
     5701 tggagcgctg cggcatcgag ttgctggaat cgggctggat cagtgacggc aacatcgaga
     5761 agttcgatga ggtggtcgag gcgacgcggc ggctgatcag cagaagcggg accaagacca
     5821 agaacgtcgc catggcgctg ccggcgtcgg tggtgatcag caagaaaatc attctaccgg
     5881 gtggcctgag cgagcgcgag atggaggtgc aggtcgaatc cgaggccaac cagtacatcc
     5941 cgttttcgct ggacgaagtg gcgctggact tcagcgtggt tggtcccagc gctacctcgg
     6001 ccggcgacgt cgaggtggtg atcgccgcgt cgcgcaagga aaaggtcgag gatcggcaag
     6061 gtctggccga ggccgccggc ctggtgccgg tggtgatgga tgtcgagacc tacgccgcac
     6121 gcctggcggt cagccgcatc attggtcgcc tgccgggcgg tggcgtcgac gccgtgatcg
     6181 ccttgttcga ggtgggcgga ctgacctcga cgctgcaggt gctgcgcaac gacgacctcg
     6241 tctacgagcg cgaccaggtg ttcggcggcg cccaactgac ccagatgctg gtgcgtcagt
     6301 acggcttcac cttcgacgag gccgaaacca agaagcgtgc gggcgagttg cccgacgact
     6361 tcggctcggt ggtgctgcag ccgttcgtgg aatcgctggc ccaggaaatc gagcgcgccc
     6421 tcaagttctt cttcaccagc acgccgcacc atcgggtcga ctacgtcctg ctggcgggtg
     6481 gcacggccag cctgccgggc ctgaccgaag tggtgacacg gctcacctcg ttcccctgcc
     6541 aggtggtcaa cccgtttgac ggcatggacg tcgctcgcac ggtgcgggaa aaacgaatgc
     6601 aacgcgaggc gccgtcctac ctgaccgcct gcggcctggc gatgcggagg ttcctgcggt
     6661 gatcctgatc aacctgctcc cgcaccggga aatggcgcgc aagaaggcgc gtcaggtatt
     6721 caacacttca ctggtcggtt cggtcttgct gggcgcgctg gtggcagggg gtgtctatct
     6781 ctggctgcag aaccagatct ccaatcagca ggagcgtaac ggcttcctga ccagtgaaat
     6841 caagaagctg gacgaggaaa tcaaggaagt ggccacgctt caggacgaga tcgctgccct
     6901 gaaggcgcgc cagcaggccg tggaggacct gcaggccaac cgcaacctgc cggtgcatct
     6961 gctgaacgaa tccgtgctgc agttgccgga cggcatctac ctcaagggca tcaaacagga
     7021 cggccagaac gtgctgctga ccggggtggc gcagtccaac gaacgcgtgt ccgaactgct
     7081 gcgcaacctg tcgcgccaca gcgactgggt gtccaagccg gagctgatcg aaatcgtggc
     7141 gtccaacctg gcgttgtcgg cgcgcgagca gcgccgggtc tacaacttca ccatgcgggt
     7201 gcagctgcag cgcgccagcg cggcgcaggc ggcggcttct gccgctccgc gcgccaagtc
     7261 ttcttgatcg gtcgcagacg atggccagca cctctatccg aaacatcgac gtctcgcagt
     7321 ggttgcgcca ggcgggcgca cagttcagcg ggctggaccg ccgcgacccc tcatcctggc
     7381 cggcggtgcc gcgctacgca ctgttcctgt gcatcgcggc cctggtggtg gggttgatgt
     7441 ggtttctctg gctgaccgac gtggagcagg aactggaagc agagcgcaac cgcgaagcca
     7501 cgctgcgcac cgaataccag ggcaagctgc agaaggccgt cagcctggaa gagctgaaga
     7561 agcagcgcgc ccaggtgatg cagtacgtga ccctgctgga aaagcagctg cccagcaagg
     7621 ccgaaatgga cgccctgctg tcggacatca accaggccgg gctgggccgc agcctgcagt
     7681 tcgacctgtt ccgcccgggg caactggtgg tcaaggacta ctacgccgag ctgccgatcg
     7741 ccgtcaaggt gaccggccgc taccacgaca tcggcgcctt cacggccgac atcgccaacc
     7801 tgtcgcgcat cgtcacgctc gacaacctgt cgatcgcccc gagcaccgac aaggaccggg
     7861 ccggcgcgct gtcgctggaa gccacggcca agacctaccg ctacctcgat cccgaggaaa
     7921 tcgccgcgca gcgcgaagcg gccaagccga aagccaagaa atgagccgcg ccgttctcat
     7981 cctgttgacc gccggggccg tgctggccgg tctggccggc tgcaccgcca acgacgagga
     8041 cctgcggcag tggatggccg agcagcggcg caccaccacg cccaaggtca caccactgac
     8101 cgagcccacc cgttacatgc cgctggccta tgccggcggc gcggccgtcg acgcattcag
     8161 cagcgaacgg ctgacccagg tgctgcgccg tgattcgggt ccgctgggtg gtggcatcgc
     8221 cctgctgacg tccgagctga accgccgcaa ggagccgctg gaagcctatc cgctggacgc
     8281 catgaccatg gtgggcagcc tggacaaggg cggccagaag gtggcgctgc tgaaggtcga
     8341 gaacctgctc catcaggtgc gcaaaggcaa ttacctgggt cagaactacg ggcgcgtgac
     8401 gcagatcact gaaagcgaaa tcaccctgag ggaaatcgtt caggacgcgg cgggtgaatg
     8461 gatcgagcgc ggcaccacgc tgcagctcca ggagaacacg aaatgaggaa gtgggtgaaa
     8521 tttctgctgc cgcgcgcggg cgttgcgggt gtcggcatgc tgctctgggg cgctgccggg
     8581 ctgagcctgg ccgagccgcg catccaggcc gtctccagtt cgatccaggg tggcaccgag
     8641 gtggtcaaga tcgacctcag cgagcccctg gcggcggtgc cggcgggctt cctggttcag
     8701 tcgcccgcgc gcatcgcgct cgacttcccc ggggtgggca gcgacgtgca gcgcagcatg
     8761 atcgagctca agcatggcaa cctgcgcggc gcgcaggtgg tgcaggcggc cgatcgcaca
     8821 cgcgtggtgc tgagcctgca gcagccggcc aactaccgcg ccgaagtgca gggcaactcg
     8881 gtgctggtgt cgctcgacca ggtcgtgacg gcgccggccg ccgcagccgc gccggcgcgc
     8941 acctttgccg aaagcggcaa cgtcgacgcc ctgccgctgc gcgacatcga cttccgacgc
     9001 ggcgccgaca gcaccggccg ggtggtggtc gacctggcca acaaccaggt gggggtcgac
     9061 attcgccagc agggccagaa cctggtggtc gaattcctgc gcaccagcct gcccgaaggt
     9121 ctgcgccgca aactcgacgt gtcggatttc ggcaccccgg tgcaaaccat caccacggta
     9181 cagagcggtg atcgggtgcg catggtgatc gagccgcgtg gactgtggga gcacagcgcc
     9241 taccagagcg acaaccagct ggtggtcgaa gtgcgccagc agaaggtcag tgccgacaag
     9301 ctgacccagg gtccgggcta ccgcggcgag aaactgtcgc tcaacttcca gaacatcgac
     9361 gtacgctctc tgttgcaggt gatcgccgac ttcaccaact tcaacatcgt cacctccgac
     9421 accgtgcagg gcaacctgac cctgcgcctg aaggacgtgc cgtgggatca ggcgctcgac
     9481 atcatcctgc agtccaagag cctgggcatg aagcgccatg gcaacgtgct gcagatcgcg
     9541 cccaaggacg aactggcggc caaggaaaag gccgagctcg aagcgcagaa ggccatccgt
     9601 gacctggagc cgctgcgcac ccagtcgttc cagctcaact acaccaaggc cgaggacatc
     9661 aagcagcagc tcacctccac ctcgggtggt ggcggtggca ccggcggcgg cagcgaaggt
     9721 cgcctgctca gctcgcgtgg cagcgtgatc gccgagccgc gcaccaacca gctgttcgtg
     9781 tccgacatcc cgtcgcgcct ggagcaaatt caggacatga tcgccaagct cgacgtgccg
     9841 gtgcgccagg tgctgatcga ggcgcgcatc gtcgaggctt ccgacacctt cggcaagtcg
     9901 ctcggcgtca agctcggcgg cgccgacctg cgaggcatcc gtggcggcga cgccggctac
     9961 ggcctgggca gcagcggcaa ccgggtggcc ttcggcggca actacaacgc catcacctcc
    10021 accaccgggc agaccgacgc ctcgggcctg acttcgctga cctcgcagtt cgtcaacctg
    10081 ccggcctcgg gcctggccgg cgccacgccg gcgagctttg ccatctcgct gttcagctcg
    10141 gccgccaacc gcttcctgaa cctggaaatc tcggccctgg aagccgacgg caagggccgt
    10201 gtggtgtcca gcccgcgcgt ggtcaccgcc gaccagacca aggcgctgat cgagcagggc
    10261 acggaattcc cgtacgagca ggccacatcc agcggcgcca cctcgatcgc cttccgcaag
    10321 gcggtgctca agctggaagt cacgccgcag atcacgcccg agggcaacat cattctcgat
    10381 ctcgacgtca acaaggacag ccccggcacc gaggtgcgcg gtggcatgtc gatcaacacc
    10441 aagcgcgtca agacccaggt gctggtcgaa aatggcggca cggtggtgat cggcggcatc
    10501 ttcgagctga ccgaacgcga aagcgagaac aaggtgccgg tgctgggcga cgtgcccgtg
    10561 ctgggcaacc tgttcaagca gcgcacgcgc caggccgaaa agcaggaaat gctggtgttc
    10621 atcaccccca agatgatctc ggatcgcggt gcggtgcgct gagcgcctcc aggaaacagg
    10681 gcggaaactc tcttttttta accaggttga ccttatgcga aagttgattg gatgggtggg
    10741 gctggcagcg gccacgttgc tgacggcgtg cggtggtggc ggcggcagcc cgggcgacac
    10801 cccggagtcc tacaccatca ccctgcgtgc cgccaagacc agtctgccgc tgaacgtggc
    10861 ccattacccg gcgggcatcg gcgtcgacgc gccgttcacc accacgctgt acgtgaacgc
    10921 caagaagggt gatgcgccga ttcccggcgg tgaggacatc tttgcctgca acaccagcta
    10981 cggcctggat tccggcccgc tgtactacct ggacggcgac gacgaacacg aggacgacga
    11041 cggcaacccg ctggcgtacc gcagcatcac gctgggctcc aacgcgggcg gcaactcctt
    11101 ccatttccat gccggcaacc aggcgggcgt ggccaccatc acctgctcgg tgcatgaccc
    11161 gcgcgccaac cgcgaggtca gcgccagcgt ggacatcacg gtcggcggtg gctccggcac
    11221 cggcatggcc gccagcatcc gcggcgtggc ccagcacgcc accctgggca cccagggcaa
    11281 cctgagcaac ctgcgcacca gtacggccat cagcgcctat gtgtgggatg acgccaacca
    11341 gcccgtaccg agcaacggca aacccaatgt gcaggccagc atcgttggca gcgtcgcgcc
    11401 gggcgccccg gcctggggcg cgcgcctgct ggccggtgcc cagcagggca gcacggtgca
    11461 ggtgcccacc accggtgggg tcgggctgtt cgcgctgtcc agcggtccca atcgtggccc
    11521 gatcctgctc gaactcaagg ccgaccggcg cgacaacgac gtcagcaacg gcatccagga
    11581 cgaagtgcgc cacctgctgg tggtgtgggc ggtcgagggt gtggcggcta ccccgctgag
    11641 cctgaaaccg gtcgaattcg acgtgcccaa cgccacgccg ttcggcgcgg tcctgagcgt
    11701 caccggtggc gaagcgccct acacctggac ggcgctggac gccctgccca cgggtctgtc
    11761 gctcagcagc ggcggtgtgc tgcagggcac gccgcgcgtg ccgccgggca cctacgtggt
    11821 gcgtgtgcgc gtcaccgacg ccctggacaa cacggccgag acttccgtca cgctgaagat
    11881 caccggcccg atgacgccgc cgctggtgtt cacggcatcg gccatcacgg ccgaggtcaa
    11941 tctgccgttc tcctacgcct tcacggcttc gggtggcacg ccgccttaca cctgggcggc
    12001 cctggggccg ctgccggccg gcctgtcggc ccccccgtcg ttcggcgccg acggtgtgat
    12061 caccggcacg cccaccacgc cgggggccta caccgtcgcg gttcgggtga ccgacagcgc
    12121 cggcgcctcg gtgacgcaga acgtggcgct caccgtggac acgccctgac ccagggcggc
    12181 ggcgggctgc cggtgcggcg gcccccgccg ccgcgcaggc cacacgccgt catggcgcta
    12241 tcctccccgg ctggtccggg gattttttta tgcacatctt tctggtcggc atgcccggca
    12301 gcggcaaatc caccgtcggc cgcgcgctgg cgcggcggtt gcagctgccc tttgtggacg
    12361 cggacacggt gatcgaggag cggctgggct gctccatcgg cgactatttc gatcaccagg
    12421 gcgaggccgc cttccgcgat gtcgagcagg gtgtgatcga cgaactcacg cagggcccgc
    12481 ccggcgtgct ggccaccggg ggcggcgcgg tgctgcgccc ggccaaccgg cagcacctgc
    12541 gcgagcgcgg cacggtgatc tatctgcgca cgtcgcccga acaactgcag cgccgcctgc
    12601 gccacgacac caagcgcccg ctgctgcagg tggccgaccc gctggcgcgc ctgcgcgccc
    12661 tgtatgccga gcgcgacccg ctgtaccgcg agtgcgcgca ctatgtgctg gacgggcatg
    12721 gcgcttcgca ggccatgctg gtggggcgca tcgtcatgca gctggaactg tcgtcgcccg
    12781 cgccccccga ccctgccggg ggcgggtgaa ccccacacta cactgcggcc catgcaagct
    12841 tcatcttcgg tcagccaggt gcgcattgcg ctcggcgagc gcacctaccc catcctgatc
    12901 ggcgccggcc tgctggccga tcccggcagc taccaggccg cgccgtccgg cggcgacgcc
    12961 ctgatcgtca ccaacaccac ggtggcgccg ctgtacgccg aacggctggc gcgcgcgctg
    13021 gccggccgcc agcgccaggt gcaccaggtg gcgctgcccg acggcgagca gcacaagcac
    13081 tgggaaaccc tgaaccgcat cttcgacgtg ctgctgggcc acggcgccga ccgcaagacc
    13141 gtgctgtacg ccctgggcgg cggcgtggtg ggcgacatga ccggctttgc cgccgcctgc
    13201 tacatgcgcg gcgtgccctt cgtgcaggtg ccgaccacgc tgctggcgca ggtggattcg
    13261 tcggtgggcg gcaagaccgc catcaaccac ccgctgggca agaacatgat cggcgccttc
    13321 taccagccgc ggctggtgct gtgcgacctg gacgtgctcg ccagcttgcc ggcacgcgag
    13381 ctgtcggccg gcctggccga ggtcatcaag tacgggccga tcgccgacat ggcgttcttc
    13441 gactggatcg aggccaacgt cgatgccttg cgcgcctgcg accgcgacgc actggcgcac
    13501 gccgtcgagc gcagctgcca gatcaaggcc gaggtggtgg gccaggacga gcgcgaaagc
    13561 ggcctgcgcg ccatcctcaa cttcggccac accttcggcc acgccatcga ggccggcctg
    13621 ggctacggcg cctggctgca cggcgaagcg gtgggcgccg gcatggtgat ggcggcgcag
    13681 ctgtcgcacg cgctggggct ggtcgacgcc gccttcgtgc agcgcctcac cgcgctgatc
    13741 gcgcgcgccg gcctgcccac gcgcggcgcg gtgctcgacg cgcacgacaa cgccggccgt
    13801 tacctgcagc tgatgcggct ggacaaaaag gccgagggcg gcgaaatccg cttcgtcgtc
    13861 atcgatggcc ccggccgcgc cgccgtgcgc gccgcgcccg acgcgctggt gcgccaggtg
    13921 atcgagcgct gctgcctgtg aaactatgat attgatagct gcttgcgctt gatgggcaag
    13981 cgctggagcc cgatttgatt cataacccgc cggccctgct gcgctgcgcc agcgacccgg
    14041 cccgctcgcg tggacggcgc tttgcggtca ccccggcgcc cacgcgcaac gacttccagc
    14101 gcgaccgcga ccgcatcgtg cactccaccg ccttccggcg cctggtctac aagacccagg
    14161 tgttcctcaa ccacgagggc gacctgttcc gcacccggct gacgcattcg ctggaagtgg
    14221 cgcaactggc gcgcgacatc gcccgcgcgc tcggcctgca cgaggacctg gtggaagcca
    14281 tcgcgctggc gcacgacctg ggccacacgc ccttcggcca cgccggccag gacgcgctgc
    14341 acgaatgcat ggccgcgcac ggcggcttcg agcacaacct gcaaagcctg cgcgtggtcg
    14401 accagctgga gcagcgctat ccgcaccacg acggcctgaa cctgagcttc gagacgcgcg
    14461 aaggcatcct caagcactgc tcggcggccc atgcgcgcgc catcgaggcg cgcgaaccgg
    14521 gcggcgtggc gcgccgcttc atcgaccgca cccagcccag cctggaagcg cagctggcca
    14581 acctggccga cgagatcgcc tacaacgcgc acgacatcga cg
//
LOCUS       DAOOOG010000044        14600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4413_length_14600_cov_23.749330, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000044 DAOOOG010000000
VERSION     DAOOOG010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14600
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4413_length_14600_cov_23.749330"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="PK242_05240"
     CDS             complement(<1..1419)
                     /locus_tag="PK242_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOZ93454.1"
                     /translation="MGKITGFLEFERIEERSEAVPARLKTWKEFVIGLDGAQARTQAA
                     RCMDCGTPFCNHGCPVNNLIPDFNDLIYHDDWRRAIDVLHQTNNFPEFTGRVCPAPCE
                     AACTLNINDDAVGIKSIEHAIIDRAWAEGWVTPQPAAIKTGKKVAVIGSGPAGLAAAQ
                     QLARAGHAVTVFEKNAQPGGLLRYGIPDFKLDKGHIDRRIAQMSAEGVVFRTGVLVGE
                     LDPDSKITRHGEQTLSAEQLLAEFDAVLLAGGSEVSRDLPVPGRALDGVHFAMEFLPQ
                     QNRVVAGDKLKGQIHAKGKHVIVIGGGDTGSDCVGTSNRQGAASVTQFELMPMPPEQE
                     DKPLTWPYWPYRLRTSSSHEEGCERQFAIATKEFVAGTGKDKGRIVGLKTVEVEWAGG
                     KMTEVPGTEKTWKADLVLLAMGFVHPVGRVLDAFGIDKDARGNARAGTDVVHGYRTSV
                     ERVFAAGDMRRGQSLVVWAIREG"
     gene            complement(1444..6180)
                     /locus_tag="PK242_05245"
     CDS             complement(1444..6180)
                     /locus_tag="PK242_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOZ93455.1"
                     /translation="MSHPLPAPHEEILAAAAGGLYQPTHEHDACGVGFIAHIKGHKTH
                     DVVTQGLRILHNLDHRGAVGADKLMGDGAGILIQIPDEYYRAEMAAQGVTLPPPGEYG
                     VGMIFLPKEHASRLACEHELERAVRDEGQVLLGWRDVPVDRDMPMSPTVRAKEPVIRQ
                     IFIGRGRDVIVPDALERKLYVIRKTASAAIQRLRLTHSHEYYVPSMSCRTVIYKGLLL
                     ADQVGQYYRDLADPRTVSALALVHQRFSTNTFPEWPLAHPYRFVAHNGEINTVKGNFN
                     WMRAREGVMRSPVLGEDLQKLYPISFEGQSDTATFDNALELLVSAGYPLAQAMMMMIP
                     EAWEQHETMDERRRAFYEYHAAMLEPWDGPAAMVFTDGKQIGATLDRNGLRPARYIVT
                     DDDHVIMASESGVLPIPESRIVSKWRLQPGKMFLIDLEQGRIVNDEELKTLLSNARPY
                     RQWIENVRVRLADTPSDGARPSYTETLLDRQQAFGYTQEDIKFQLLPMAQQGEEAIGS
                     MGNDTPLAVLSDKNKPLYNYFKQLFAQVTNPPIDPIREAIVMSLHSFIGPKPDLLDIN
                     AVNPQMRLEVEQPILDFDDMARLRAIERHTGGKFKTYTIDITYPLSWGREGVEAKLAS
                     LCAEALDAIRGGHNILVISDRRMDRDHIAIPALLALSAIHHHLVHAGLRTTAGLVVET
                     GSAREVHHFAVLAGYGAEAVHPYLAMETLTALHEATPEGPSPKKAIQNYVKAIGKGLS
                     KIMSKMGISTYMSYCGSQIFEAIGLKEDFVAKYFRGTATQVGGIGVFEVAEEALRQHL
                     AAFGDDPTLQNMLDAGGDLAWRTRGEEHMWTPDAIAKLQHSVRGGKWDTYKEYAQIIN
                     DQSRRHMTLRGLFEFKVDPARAIPLDQVEPAAEIVKRFATGAMSLGSISTEAHTTLAL
                     AMNRIGGKSNTGEGGEDPARYRNELKGIPIAAGTRVSEVIGSRVIESDYAMQAGDSLR
                     SRIKQVASGRFGVTAEYLVSADQIQIKMAQGAKPGEGGQLPGGKVSEYIGFLRYSVPG
                     VGLISPPPHHDIYSIEDLAQLIHDLKNVNPQASISVKLVSEVGVGTIAAGVAKCKADH
                     VVIAGHDGGTGASPWSSIRHAGTPWELGLAETQQTLVLNRLRSRIRVQADGQMKTGRD
                     VVIGALLGADEFGFATAPLVAEGCIMMRKCHLNTCPVGVATQDPVLRRRFSGKPEHVI
                     NYFFFVAEEVRTIMAQLGLRRFDELIGRADLLDTRRAITHWKAQGLDFSRVFHQPAVP
                     SEVPRLQTETQDHGLARALDHKFIERCQPALERGERVQLMDEVRNVHRSVGAMLSGEL
                     VRRHPEGLPDHTVVMQLEGTGGQSFGAFLARGVTLHLIGDANDYTGKGLSGGRIVVRP
                     SIDFRGDATRNIIVGNTALYGATSGEAFFRGVAGERFAVRLSGATAVVEGTGDHGCEY
                     MTGGTVVVLGSTGRNFAAGMSGGVAYVFDEDGRFSERCNTSMVALEKVPPRDAQEGIA
                     AGWHRAMADEDLLRKLIEEHLRWTGSLRAREILDHWADSRARFVKVMPREYQRVLSAH
                     AEQAAIATADSALVKAKASAALASGQ"
     gene            complement(6447..7118)
                     /locus_tag="PK242_05250"
     CDS             complement(6447..7118)
                     /locus_tag="PK242_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ93456.1"
                     /translation="MARLPRLVLPGQPHLVLQRTLDHRPAFVDDEDRAQFMVVLRTAL
                     ETEAVQLHAFALRDHELLFLVTPDQPDALARLMQAIGRRYVTAYNRRHARSGPLWDGR
                     FRTSAVEPGEHTLTAMTWIEQATLTPGITSAPHHAGTQPNPLITDPPEYWQLGNTPFE
                     RESAWRERLARGVAGTTEARLRRLVLGGWALGSPEFICSADATGRPAAPRRPGRPKRA
                     ASNPR"
     gene            complement(7243..8706)
                     /gene="ntrC"
                     /locus_tag="PK242_05255"
     CDS             complement(7243..8706)
                     /gene="ntrC"
                     /locus_tag="PK242_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HOZ93457.1"
                     /translation="MKPIWVVDDDQSIRFVLERALSREHLPVRSFSRPQDVLNEFDAG
                     VEPQVLVTDIRMPGSSGIELLTEAKRRHPQLPVIIMTAYSDLDSAVGAFRSGAFEYLP
                     KPFDVNRAVELIRRAAEDHSLRDQPHADDSDQQPLMLGEAPAMQEVFRAIGRLSQSES
                     TVLITGASGTGKELVALALHRHSPRARGPFVAINTAAIPKDLLESELFGHERGAFTGA
                     QAMRRGRFEQAAGGTLFLDEIGDMPLELQTRLLRVLSDGQFYRVGGHQPLRADTRVIA
                     ATHQDLEERVRQGTFREDLFHRLNVIRLRLPPLNERREDIPLLTRHFLARSARELGVE
                     SKHISPAALERLTQFDFPGNVRQLENLCRWLTVMAPGQRIEAEDLPADLLPRGEPRPL
                     TGEEKATAAITPPHPDAPPSAAAPADWTVALAQEARTRLTAGESGVWDSLAGRFEAAL
                     LHTALGLTHGRRAEAAQKLGIGRNTITRKIQELGLDD"
     gene            complement(8703..9767)
                     /gene="glnL"
                     /locus_tag="PK242_05260"
     CDS             complement(8703..9767)
                     /gene="glnL"
                     /locus_tag="PK242_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HOZ93458.1"
                     /translation="MVALVRVDGTCLLVNAALEHAVRQSRRKLVQARALDWFVDPAPL
                     QAALHSTASHHLAVSRFEGALLRQPPGAGELPVHVVVTPVGRGGPEGAVMVEMIEIER
                     QVQLDREERAQDLIQAHQELLRNLAHEIKNPLGGIRGAAQLLAMEFTSPEYGEYTEVI
                     IREADRLQALVDRLLAPHRGMPQWSDFNIHEVCERVRALVLAEFPRGLQVQRDYDTSL
                     PELRGDRERLIQALLNIVRNAAQALAARIVTGDARIVLRTRIARQVTLNGRRFRLAIQ
                     LQVEDNGPGIPPALRERIFQPLVSGHAAGSGLGLTLAQGFIQQHGGTIECESQPGATR
                     FTLLIPLPDPTACRDIEGPP"
     gene            complement(10098..11315)
                     /locus_tag="PK242_05265"
     CDS             complement(10098..11315)
                     /locus_tag="PK242_05265"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOZ93459.1"
                     /translation="MRLNPHLVAMPGDYLFAEVARRVQAYAPSAPRPIIRLGIGDVTQ
                     PLAPAITQAMVEAAQEMGTPEGFHGYPPDQGYPFLQQAIIDADYAPLGVRLELDEVFV
                     TDGAKTDTSALPELLAADARIGVTDPVYPAYIDANAMAGRLGRHEGGAWSALTILPCR
                     AENDFVPELPAQPVDMLYLCYPNNPTGTVLTREQLTRFVRYAQDTGTLILFDAAYKAY
                     ITDPAIPRSIYEIEGADQVAVECCSFSKSAGFTGTRCAYTIIPKALKARIDGQSVSLN
                     ALWRRRVACRQNGVSYPIQRAAAAALSPQGQRQVAAQVQTYMDNAHHMVATLQGAGLQ
                     VFGGVHAPYVWLRIPGQRSSWEFFDHLLHQAQVVGTPGSGFGQAGEGYLRLTAFNTAA
                     NTREALERVLAVL"
     gene            complement(11317..12153)
                     /gene="dapF"
                     /locus_tag="PK242_05270"
     CDS             complement(11317..12153)
                     /gene="dapF"
                     /locus_tag="PK242_05270"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOZ93460.1"
                     /translation="MKFTKMHGLGNDYVYVNAFDETVADPAAMAVAVSRPHFGVGADG
                     LVLIGPSAEADFAMRMFNADGSEGEMCGNAVRCIGKYVHDRGLTRKTEVRLSTLGGIK
                     TLQLRLKDGSVDQVTVDMGEPAFSPASLPVEWPGERIVNQPLTIDGQTHHITCVSMGN
                     PHAVIFVDDPKAIDVHGIGRQIENHPLFPRRTNVEFVRVRRRDLLEMRVWERGSGETL
                     ACGTGAAASLVAAVLNGLADRSAVVQLTGGDLQVTWDSSDNHVYQTGPASFVFDGVWL
                     GD"
     gene            complement(12166..13581)
                     /gene="glnA"
                     /locus_tag="PK242_05275"
     CDS             complement(12166..13581)
                     /gene="glnA"
                     /locus_tag="PK242_05275"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOZ93461.1"
                     /translation="MSHTAADVMTMIQEQNIRYIDLRFTDTRGKEQHVTVPVSAFDED
                     KFSSGHPFDGSSIAGWKGIQASDMLLLPDPATANVDPFFDDPTLILTCDVLEPGDGKP
                     YDRDPRSLARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVRWQADMSGCFVKIDSEEAA
                     WNTGTAYEQGNHGHRPGVKGGYFPVPPVDSFQNMRSQMCTLLESLGIPVEVHHHEVAT
                     AGQMELGTKFSTLVQRADWLQLQKYVIHNVAHAHGKTATFMPKPIVGDNGSGMHVHQS
                     VWKDGRNLFAGDGYAGLSEFALYYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPVK
                     LAYSARNRSASIRIPYVANPKGRRIEVRFPDPTANPYLAFSALLMAGLDGVENRIHPG
                     EAMDKDLYHLPPEEDALIPTVCHSLDQALEHLDKDRGFLTKGGVFSDAMIDAYLELKM
                     AEVTRFRMTTHPIEFDMYYSL"
     gene            complement(13825..14220)
                     /locus_tag="PK242_05280"
     CDS             complement(13825..14220)
                     /locus_tag="PK242_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOZ93462.1"
                     /translation="MSFVEHSAAGVAILLWASDPAQPERLATPFFHAAAAAAMDVPVE
                     IYFSAASVQLLAPGVAAALRASPRHDKTILDALREAVAHGARLLACTDALHAHGLAPS
                     GLIPECHGQGGAVQFMARALDTRWRTLVF"
     gene            complement(14217..>14600)
                     /locus_tag="PK242_05285"
     CDS             complement(14217..>14600)
                     /locus_tag="PK242_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93463.1"
                     /translation="TATVGITELGIRLSGEIYMCRAKRIGTEVAQGGTLAVVELAKAI
                     VAVKSPVTGTVIEANAALADRPQLVHRDPYGTGWIARLRLADFQADQPALLHGDAVAP
                     AMARHALAHGQQLAGTTRHPSHPAP"
BASE COUNT         2102 a         4704 c         5247 g         2547 t
ORIGIN      
        1 gccttcgcgg atggcccaga cgaccaggct ctggccgcga cgcatgtcgc cggcggcaaa
       61 cacgcgctcc acactggtgc ggtaaccgtg gaccacatcg gtgccggcgc gggcgttgcc
      121 gcgcgcatcc ttgtcgatgc cgaaggcgtc cagcacacgg ccgaccgggt gcacgaagcc
      181 catcgccagc agcaccagat cggccttcca ggtcttttcg gtgcccggga cctcggtcat
      241 cttgccgccg gcccattcga cctcgaccgt cttcagcccg acgatgcgtc ccttgtcctt
      301 gccggtgccg gcgacgaatt ccttggtggc gatggcgaac tggcgctcgc aaccttcttc
      361 gtggctggaa ctggtgcgca ggcggtaggg ccagtacggc caggtcaggg gcttgtcctc
      421 ctgctcgggc ggcatgggca tgagctcgaa ctgggtgacg ctggcagcgc cctgccggtt
      481 gctggtgccg acacagtcgc tgccggtgtc gccaccaccg atgacgatga cgtgcttgcc
      541 cttggcatga atctgcccct tgagcttgtc accggccacc acccggttct gctgcggcag
      601 gaactccatc gcgaagtgca cgccgtcgag cgcgcgaccc ggcaccggca ggtcacggct
      661 gacctcactg ccaccggcca gcagtaccgc atcgaactcg gccagcaact gctcggcgct
      721 gagcgtttgt tcgccgtgac gggtgatctt gctgtccggg tccagctcgc cgaccagcac
      781 gccggtgcgg aacaccacac cctcggcgct catctgcgcg atgcggcggt cgatgtggcc
      841 cttgtccagc ttgaaatccg gaatgccata gcgcagcagg ccgccaggct gggcgttctt
      901 ttcgaacacc gtcaccgcgt gcccggcgcg ggcaagctgc tgggccgccg ccagcccggc
      961 cggtccggag ccgatcaccg ccaccttctt gccggtcttg atggccgccg gctgcggcgt
     1021 cacccagcct tcggcccagg cgcggtcgat gatggcgtgc tcgatgctct tgatgccgac
     1081 ggcgtcgtca ttgatgttga gcgtgcaggc ggcttcgcag ggtgccgggc agacgcgccc
     1141 ggtgaactcg gggaagttgt tggtctggtg cagcacgtcg atggcacggc gccagtcgtc
     1201 gtgatagatg aggtcgttga agtccggaat caggttgttg accgggcagc cgtggttgca
     1261 gaacggggtg ccgcaatcca tgcagcgtgc cgcctgcgtg cgcgcctgcg cgccgtccag
     1321 gccgatgacg aactccttcc aggtcttcag tcgcgccggc acggcctcgc tgcgttcctc
     1381 gatgcgttcg aattccagaa agccggtgat ctttcccatg atgctgtctt gcttgaatgg
     1441 ttgtcattga cccgatgcca gcgcggcgct ggcctttgcc ttcaccagcg cgctgtcggc
     1501 ggtggcgatg gcggcctgtt cggcatgcgc cgagagcacc cgctggtact cacgcggcat
     1561 caccttgaca aagcgtgcgc gtgaatcggc ccagtgatcg aggatttcgc gcgcgcgcag
     1621 cgaacccgtc cagcgcaggt gctcctcgat cagcttgcgc agcaggtcct catcggccat
     1681 ggcgcggtgc cagccggcgg cgatgccttc ctgggcgtcg cgcggcggca ccttctcgag
     1741 ggccaccatg ctggtgttgc aacgttcgct gaagcgaccg tcctcgtcga acacgtaggc
     1801 cacgccaccg ctcatgccgg cggcgaagtt gcggccggta ctgcccagca ccaccacggt
     1861 gccgccggtc atgtattcac agccgtggtc gccggtgcct tccaccacgg cggtggcgcc
     1921 cgacaggcgc accgcgaagc gttcgcccgc cacgccgcgg aagaaggctt cgccgctggt
     1981 ggcgccgtag agcgcggtgt tgccgacgat gatgttgcgg gtggcgtcgc cgcggaagtc
     2041 gatgctcggc cgcaccacga tgcgtccgcc cgacaggccc ttgccggtgt agtcgttggc
     2101 gtcaccgatc agatgcaggg tgacgccgcg cgccaggaag gcgccgaagc tctggccacc
     2161 cgtgccttcg agctgcatca cgacggtgtg gtcgggcagc ccttcggggt ggcggcgcac
     2221 cagctcgccc gacagcatgg cgcccacgct gcgatgcacg ttgcgcacct cgtccatcaa
     2281 ctggacccgc tcgccgcgct ccagcgccgg ctggcaacgc tcgatgaact tgtggtccag
     2341 ggcgcgggcc aggccgtggt cctgcgtttc cgtctgcagc cgcggcactt ccgacggcac
     2401 cgccggttgg tggaacacac ggctgaagtc cagcccctgg gccttccagt gggtgatggc
     2461 tctgcgcgta tcgagcagat cggcgcgccc gatcagttcg tcgaagcggc gcaggcccag
     2521 ctgcgccatg atggtgcgca cttcttcggc gacgaagaag aagtagttga tgacgtgctc
     2581 gggcttgccc gagaacctgc ggcgcagcac cggatcctgg gtggccacgc cgaccgggca
     2641 ggtgttgagg tggcacttgc gcatcatgat gcagccctcg gccaccaggg gtgcggttgc
     2701 gaagccgaac tcgtcggcgc ccagcagcgc gccgatcacg acgtcacgcc cggttttcat
     2761 ttgcccgtcg gcctgcacgc ggatgcggct gcgcaggcgg ttgagcacca gggtctgctg
     2821 ggtctcggcc aaccccagct cccacggcgt gccggcatgg cggatgctgg accagggcga
     2881 ggcaccggtg ccgccgtcgt ggccggcaat caccacatgg tcggccttgc acttggcgac
     2941 gccggcggcg atggtgccga cgcccacttc gctgaccagt ttgacgctga tggaggcctg
     3001 cgggttgacg ttcttcaggt cgtggatgag ctgggccagg tcttcgatgg agtagatgtc
     3061 atggtgcggt ggcgggctga tcagacccac cccggggacg ctgtagcgca gaaagccgat
     3121 gtactccgac accttgcccc cgggcagttg gccgccttcg ccgggcttgg ccccctgggc
     3181 catcttgatc tggatctggt cggccgaaac caggtactcg gcggtcacac caaaacgccc
     3241 ggaagccacc tgcttgatcc ggctgcgcag gctgtcgccg gcctgcatgg cgtagtcgga
     3301 ttcgatgacg cggctgccga tcacctcgct gacgcgggtg ccggcggcga tcgggatgcc
     3361 cttgagttcg ttgcgatagc gcgccgggtc ttcgccgcct tcgccggtgt tgctcttgcc
     3421 gccgatgcgg ttcatggcca gcgccagcgt ggtgtgggct tcggtgctga tcgaaccgag
     3481 cgacatggcg ccggtggcaa agcgcttgac gatctcggcg gcgggctcca cctgatcgag
     3541 cgggatggcg cgggccgggt cgaccttgaa ctcgaacagg ccgcgcagcg tcatgtggcg
     3601 gcggctctgg tcgttgatga tctgcgcgta ctccttgtag gtgtcccact tgccgccgcg
     3661 cacgctgtgc tgaagcttgg cgatggcgtc cggcgtccac atgtgttcct cgccgcgggt
     3721 gcgccaggcc aggtcgcccc cggcgtcgag catgttctgc agcgtcgggt cgtcgccgaa
     3781 cgcggccagg tgctggcgca gcgcttcttc ggccacttcg aacacaccga tgccgcccac
     3841 ctgggtggcg gtgccacgga agtacttggc gacaaagtcc tctttcaggc cgatggcttc
     3901 gaagatctgg gatccgcagt acgacatgta ggtgctgatg cccatcttgg acatgatctt
     3961 ggacagcccc ttgccaatcg ccttgacgta gttctggatc gccttcttgg gcgaaggccc
     4021 ctcgggcgtc gcttcgtgca gggcggtcag cgtttccatc gccaggtacg ggtgcaccgc
     4081 ttcggcgccg tagccggcca acaccgcaaa gtggtgcact tcgcgcgcac tgccggtttc
     4141 caccaccagg ccggcggtgg tgcgcagtcc cgcatgcacc aggtggtggt gaatggccga
     4201 cagggccagc aacgccggga tggcgatgtg gtctcggtcc atgcggcgat cgctgatgac
     4261 gaggatgttg tgcccgccac ggatggcatc gagcgcctcg gcacacagcg aggccagctt
     4321 ggcttccacg ccttcgcggc cccaggacag cgggtaggtg atgtcgatcg tgtaggtctt
     4381 gaacttgccg ccggtgtgcc gctcgatcgc gcgcaggcgc gccatgtcgt cgaagtccag
     4441 aatgggctgc tcgacctcca gccgcatctg cgggttgacc gcattgatgt ccagcaggtc
     4501 cggcttgggg ccgatgaagc tgtgcagcga catcacgatg gcttcgcgga tcgggtcgat
     4561 cggcgggttg gtgacctgag cgaacaactg cttgaaatag ttgtacagcg gcttgttctt
     4621 gtccgacagc accgccagcg gtgtgtcgtt gcccatcgag ccgatggctt cctcgccctg
     4681 ctgggccatg ggcagcagct ggaacttgat gtcctcctgc gtgtagccga aggcctgctg
     4741 gcgatcgagc agcgtttcgg tgtagctggg gcgcgcgccg tcgctcgggg tgtcggccag
     4801 gcgcacgcgg acgttttcga tccactgccg gtatgggcgg gcgttggaca gcagggtctt
     4861 gagctcttcg tcgttgacga tgcgaccctg ttccagatcg atcaggaaca tcttgccggg
     4921 ctgcaggcgc cacttgctga cgatccggct ctcggggatc ggcagcacgc cgctttcact
     4981 ggccatgatg acgtggtcgt cgtcggtgac gatgtagcgc gccggtcgca ggccgttacg
     5041 gtcgagcgtg gcaccgatct gcttgccgtc ggtgaacacc atggcggccg ggccgtccca
     5101 cggctccagc atggcggcgt ggtattcgta gaacgcgcgc cggcgttcgt ccatggtctc
     5161 gtgctgttcc caggcctcgg ggatcatcat catcatggcc tgggccagcg ggtagccggc
     5221 cgacaccaga agttccagcg cgttgtcgaa ggtggcggtg tcgctctggc cttcgaagga
     5281 gatcgggtag agcttctgca ggtcctcgcc cagcacgggc gagcgcatca cgccctcgcg
     5341 tgcgcgcatc cagttgaaat tgcccttgac ggtgttgatc tcgccgttgt gggcgacgaa
     5401 gcggtagggg tgggccagcg gccattcggg gaaggtgttg gtcgaaaaac gctggtgcac
     5461 cagcgccagt gccgagaccg tgcgcgggtc ggccaggtcc ctgtagtact gacccacctg
     5521 gtcggccagc agcaggccct tgtagatgac ggtgcggcag ctcatgctgg gcacgtagta
     5581 ttcgtggctg tgtgtcagcc gcagccgctg gatggcggcg ctggcggtct tgcggatcac
     5641 atagagcttg cgctccagcg catccggcac gatgacgtcg cgaccgcggc cgatgaagat
     5701 ctgacggatc accggctcct tggcgcgcac cgtcgggctc atcggcatgt cgcggtcgac
     5761 cggcacgtcg cgccagccga gcaggacctg accttcgtcg cgcacggcgc gctcgagttc
     5821 gtgctcgcag gccaggcgcg aagcgtgttc cttgggcagg aagatcatgc cgacgccgta
     5881 ctcgcccggc gggggcaggg tgacgccctg tgccgccatc tcggcgcggt agtactcgtc
     5941 ggggatctgg atcaggatgc cggcgccgtc gcccatcagc ttgtcggcgc ccacggcgcc
     6001 tcgatggtca aggttgtgca ggatgcgcaa cccctgggtg acgacgtcgt gcgtcttgtg
     6061 gcccttgatg tgggcgatga agccgacccc gcaggcgtcg tgttcgtgcg tgggctggta
     6121 cagaccgccg gcagcggcgg cgaggatttc ttcgtggggg gcggggagcg ggtggctcaa
     6181 ggcttgactc caacgtgggc gtgatgaggc atggaggcta gcatgcgcaa acggggacta
     6241 caagaaatat aaaacggggt cggaacataa ataaatgtca aaaggttgga tttcataaaa
     6301 atgggggaca taacaactaa gggtgtacca atggtgcgcc gcctgatctc gggtgcgacc
     6361 gatggtgacg taagtgttgg ttatgaaacg gcttttttgt cgaccgccgc tggtgttcac
     6421 caggtgccgc cgacggacgc gggcgctcag cgcgggttgc tggcggcgcg cttggggcgc
     6481 ccaggtcggc gtggggccgc gggtcgcccg gtggcatccg cgctgcagat gaattcgggc
     6541 gagcccagtg cccagccgcc cagcaccagg cggcgcagcc gcgcctccgt ggtgccggcc
     6601 acgccgcgtg caaggcgctc ccgccaggcc gattcgcgtt cgaagggggt gttgccgagt
     6661 tgccagtatt cgggcgggtc ggtgatcagg gggttgggct gtgtcccggc atggtgcggc
     6721 gcgctggtga tgccaggtgt cagcgtggcc tgctcgatcc aggtcatggc ggtcagggtg
     6781 tgttccccgg gttccacggc actggtgcga aaccgcccgt cccacagcgg gccgctgcgt
     6841 gcgtgccgcc ggttgtaggc cgtcacgtag cgccgaccga tggcctgcat cagccgcgcc
     6901 agcgcgtcgg gctggtccgg cgtgaccaga aacagcaact cgtggtcgcg cagtgcaaag
     6961 gcgtgcaact gcaccgcttc cgtttccagg gcggtgcgca gcacgaccat gaactgcgca
     7021 cgatcctcgt cgtcgacgaa ggcgggacgg tggtccagcg tgcgttggag caccagatgg
     7081 ggctggccgg gcagcacaag gcggggcagg cgcgccatgg gaagcgttca gaaggtggac
     7141 aagaaggggt tcgctcccac ttgacttgtt cagggcgcgg gtctgaccgt gggctcgcgc
     7201 cgcctgcgcg tgtgggggcg tgccgcaggg cgtggcgagc gttcagtcat cgagtcccag
     7261 ctcctggatc ttgcgggtga tggtgttgcg tccgatgccg agcttctggg cggcctcggc
     7321 gcggcgcccg tgggtcaacc ccagggcggt gtgcagcagc gccgcttcga agcgacccgc
     7381 caggctgtcc cacaccccgg actcaccggc cgtcagccgg gtgcgtgcct cctgagccag
     7441 ggccaccgtc cagtcggcgg gcgcggcggc gctgggtggg gcgtcggggt ggggtggcgt
     7501 gatggccgcc gtcgccttct cttcgcccgt cagcggacgt ggctcaccac gcggcagcag
     7561 gtcggccggc aggtcttccg cctcgatccg ctggccaggg gccatcaccg tcagccagcg
     7621 gcacaggttt tccagctggc gcacgttgcc gggaaagtcg aactgcgtca gccgttccag
     7681 cgcggcgggg ctgatgtgct tgctctccac ccccagctcg cgtgcgctgc gggcaagaaa
     7741 atggcgcgtc agcaggggga tgtcttcgcg tcgctcattg agcggcggca gccgcagtcg
     7801 aatgacgttg aggcggtgga acaggtcttc ccggaaggtg ccctggcgca cgcgctcttc
     7861 gagatcctgg tgcgtggcgg cgatcacccg ggtgtcggcc cgcagcggct ggtggccgcc
     7921 gacccgatag aactgcccgt cactcagcac gcgcagcagg cgggtctgca actccagcgg
     7981 catgtcgccg atttcgtcca ggaacagggt gccgccggcg gcctgctcga agcgaccgcg
     8041 ccgcatggcc tgcgcgccgg tgaaggcgcc gcgttcatgg ccgaacagct cgctctccag
     8101 caggtccttg gggatggcgg ccgtgttgat ggccacgaac ggcccgcgcg cccgcggact
     8161 gtgacggtgc agggccagcg ccaccagctc cttgccggtg ccgctggcgc cggtgatgag
     8221 caccgtgctt tcactttggc tgagccgtcc gatggcgcga aacacctcct gcatggccgg
     8281 cgcctcgccc agcatcaggg gctgctgatc gctgtcgtcg gcgtgcggct gatcgcgcag
     8341 gctgtggtct tcggcggcgc ggcgaatcag ttcgaccgcg cggttgacgt cgaagggttt
     8401 tggcaggtac tcgaaggcgc cgctgcgaaa cgcgcccacc gcgctgtcga ggtcgctgta
     8461 ggcggtcatg atgatgaccg gcagctgggg gtggcggcgc ttggcttcgg tcagcagctc
     8521 gatgccgctg ctgcccggca tgcggatgtc ggtcaccagc acctggggct cgacccccgc
     8581 atcgaactcg ttgagcacgt cctgcggtcg gctgaagctg cgcacgggta ggtgctcgcg
     8641 gctcagcgcg cgttccagta cgaaacggat ggattgatcg tcgtcgacga cccagatcgg
     8701 cttcatggcg ggccctcgat gtcgcggcag gcggtagggt cgggcagggg gatcagcagc
     8761 gtaaagcggg tggcgcccgg ctggctttcg cattcgatcg tgccgccatg ctgttgaatg
     8821 aacccctgcg ccagcgtcag acccagcccg ctgccggcgg catggccgga caccagcggc
     8881 tggaagatgc gctcccgcag ggcggggggg atgccggggc cgttgtcctc cacctgcagc
     8941 tggatcgcca ggcggaagcg ccggccgttg agcgtgacct gacgcgcgat gcgggtgcgc
     9001 agcacgatgc gcgcatcgcc ggtgacgatg cgcgcggcaa gcgcctgcgc ggcgttgcgc
     9061 acgatgttga gcagcgcctg gatcagccgc tcgcggtcgc cgcgcagctc cggcagcgag
     9121 gtgtcgtagt cgcgctgcac ctgcaggccg cgcggaaact cggccagcac cagcgcacgc
     9181 acgcgctcgc agacctcgtg gatgttgaag tcgctccact gcggcatgcc gcggtgcggt
     9241 gccagcagcc ggtcgaccag cgcctgcagc cggtcggcct cgcggatgat gacctcggtg
     9301 tactcgccgt actcggggga ggtgaattcc atcgccagca actgggcggc gccgcggatg
     9361 ccgcccagcg ggttcttgat ctcatgggcc agattgcgca gcagttcctg gtgggcctgg
     9421 atcaggtcct gtgcgcgctc ctcgcggtcg agctgcacct gccgttcgat ctcgatcatc
     9481 tcgaccatca ccgcgccctc cgggccgccg cgtcctaccg gggtcaccac cacatgcacc
     9541 ggcaattcac ccgcgccggg gggctggcgc aacagggcgc cttcgaagcg gctgaccgcc
     9601 aggtgatggc tggcggtgct gtgaagcgcc gcctgcagcg gcgccgggtc gacgaaccaa
     9661 tcgagcgcgc gcgcctgcac cagcttgcgg cgcgattgcc gaaccgcgtg ctccagcgcc
     9721 gcgttgacca gcaggcaggt gccatcgacc cgcaccagcg ccaccatcag ggccagacga
     9781 tcgtaggcct cctggtcctg tggcgagatc gcagtgggtg ccgcctccgc accagccgcc
     9841 acgggagcag gcggctcagg gcttggggtc gacctggtca tgacgaaggc agtgcgagtc
     9901 ggatccaagg ggcgtggtgc ctgggcgtca gcggcaccgg gttcgggggc agggcatgtc
     9961 agtgtagcgg gcgctgctgg cctggccggc acccgccgtg cccgcccccg ggggtgcagg
    10021 ggcggcggcg acagggggcg tcgcgccgct gctccggtct gcccgttcgg gcgtgccatg
    10081 ggcgccccgc cacagtgtca cagcaccgcc agcacgcgct cgagcgcttc gcgcgtgttg
    10141 gcagcggtgt tgaacgcggt caggcgcagg tagccttcgc cggcctggcc aaagcccgac
    10201 ccgggggtgc cgaccacctg agcctggtgc agcagatggt cgaagaactc ccacgagctg
    10261 cgctggcccg gaatcctgag ccacacgtag ggtgcgtgca caccgccgaa cacctgcagg
    10321 cccgcgccct ggagtgtcgc caccatgtgg tgggcgttgt ccatgtaggt ctgcacctgg
    10381 gccgccacct ggcgctgccc ctgcggcgac agggccgccg cggcggcgcg ctggatcggg
    10441 taggacacgc cgttctgccg gcacgccacg cggcgacgcc acagcgcgtt gagcgacacc
    10501 gactggccgt cgatgcgggc cttgagcgcc ttggggatga tggtgtaggc gcagcgcgtg
    10561 ccggtgaacc cggccgactt ggagaacgaa cagcactcca ccgccacctg gtcggcgccc
    10621 tcgatttcgt agatcgagcg cgggatcgcc gggtcggtga tgtaggcctt gtaggcggcg
    10681 tcaaacagga tcagcgtgcc ggtgtcctgg gcgtatctga cgaagcgggt cagctgctcg
    10741 cgggtcagca cggtgccggt ggggttgttg gggtagcaca ggtagagcat gtccaccggc
    10801 tgtgccggca gctcgggcac gaagtcgttc tcggcccggc acggcaggat ggtcagtgcg
    10861 ctccatgcgc cgccctcatg gcgtccgagc cggccggcca tggcgttggc gtcgatgtag
    10921 gccggataga ccgggtcggt gacgccgatg cgcgcgtccg cggccagcag ctcgggcagg
    10981 gccgaggtgt cggtcttggc gccgtcggtg acgaacacct cgtccagttc cagccgcacg
    11041 cccagggggg cgtagtcggc atcgatgatg gcctgctgca ggaacgggta gccctgatcg
    11101 ggcgggtagc catgaaagcc ctcgggcgtg cccatttcct gggccgcctc gaccatggcc
    11161 tgagtgatgg cgggggccag gggctgcgtc acgtcgccga tgcccaggcg gatgatcgga
    11221 cgcggtgccg agggcgcata ggcctggacg cggcgcgcca cttcggcaaa caggtagtcg
    11281 ccgggcatgg cgaccaggtg ggggttcaga cgcatttcaa tctccgagcc acacgccatc
    11341 gaacacgaag ctggcggggc ctgtctggta aacgtggttg tcgctgctgt cccaggtgac
    11401 ctgcagatcg ccgccggtca gctgcaccac cgcgctgcga tcggccaggc cgttcagcac
    11461 ggcggccacc aggctggccg cggcgccggt gccgcaggcc agggtttcac cgctgccgcg
    11521 ctcccatacc cgcatttcca acaggtcgcg ccgccgcacg cgcacgaact cgacgttggt
    11581 gcgccgcggg aacagcgggt ggttctcgat ctgccggccg atgccgtgca cgtcgatcgc
    11641 cttcgggtcg tcgacgaaga tgaccgcatg ggggttgccc atgctgacgc aggtgatgtg
    11701 gtgggtctgg ccgtcgatcg tcagcggctg gttgacgatg cgctcgccgg gccactcgac
    11761 cgggaggctg gccgggctga acgccggttc acccatgtcc accgtcacct ggtcgacgct
    11821 gccatccttc agccgcagtt gcagggtctt gatgccgccc agggtcgaca gcctgacttc
    11881 ggtcttgcgc gtcagcccgc ggtcgtgtac gtacttgccg atgcagcgca cggcgttgcc
    11941 gcacatttcg ccttcggagc cgtcggcgtt gaacatgcgc atcgcgaagt cggcctccgc
    12001 cgagggcccg atcagcacca gcccgtcggc gccgacgcca aagtgcgggc gtgacacggc
    12061 gacggccatg gccgccgggt cggccacggt ttcgtcaaag gcattgacgt agacgtagtc
    12121 gttgcccagg ccatgcatct tggtgaattt catgccaccc gttccttaca ggctgtagta
    12181 catgtcgaac tcgatcgggt gggtggtcat gcgaaagcgc gtgacttcgg ccatcttgag
    12241 ttcgaggtag gcgtcgatca tggcgtcgga gaacacaccg cccttggtca ggaagccgcg
    12301 gtccttgtcc aggtgctcca gcgcctgatc caggctgtgg cacacggtgg ggatcagcgc
    12361 gtcctcttcg ggcggcaggt ggtacaggtc cttgtccatc gcctcgccgg ggtggatgcg
    12421 gttttccacg ccgtccagac ccgccatcag cagcgccgag aaggccaggt aggggttggc
    12481 ggtggggtcg gggaagcgca cctcgatgcg ccggcccttg gggttggcca cgtaagggat
    12541 gcggatcgag gccgagcggt tgcgcgcgct gtaggccagc ttcaccggcg cttcgaagcc
    12601 gggcaccagg cgcttgtagc tgttggtgcc ggggttggtg atggcgttga gcgcacgggc
    12661 gtgcttgatg atgccgccga tgtagtacag cgcaaactcg ctcaggccgg cgtagccgtc
    12721 gccggcgaac aggttgcggc cgtccttcca caccgactgg tgcacgtgca tgcccgagcc
    12781 gttgtcgccg acgatgggct tgggcatgaa ggtggccgtc ttgccatggg cgtgggccac
    12841 gttgtggatc acgtacttct gcaactgcag ccagtcggcg cgctgcacca gggtgctgaa
    12901 cttggtgccc agctccatct gaccagcggt ggccacctcg tggtggtgca cctcgaccgg
    12961 gatgcccagc gattcgagca gcgtgcacat ctgcgagcgc atgttctgga agctgtccac
    13021 cggcggcacc gggaagtagc cgcccttgac gcccgggcgg tggccgtggt tgccctgctc
    13081 gtaggcggtg ccggtgttcc aggcggcttc ctcggagtcg atcttgacga agcagcccga
    13141 catgtcggcc tgccagcgca cgccgtcgaa gacgaagaac tcgggctcgg ggccgaagta
    13201 ggcggtgtcg ccgatgccgc tgctcttgag gtaggcctcg gcgcgccggg ccagcgagcg
    13261 cgggtcgcgg tcgtagggct tgccgtcgcc cggctccagc acgtcgcagg tcaggatcag
    13321 ggtggggtcg tcgaagaacg ggtcgacgtt ggcggtggca gggtcgggca gcagcagcat
    13381 gtcggacgcc tggatgcctt tccagccggc gatggacgag ccgtcgaacg ggtgcccgct
    13441 gctgaacttg tcttcgtcga aggcagacac cggcaccgtg acgtgctgct ccttgccccg
    13501 ggtgtcggtg aagcgcagat cgatgtagcg gatgttctgc tcctggatca tcgtcatgac
    13561 gtcggcggcg gtatggctca ttgggttggc tccatgaagg gttgggatgc cagcgatcaa
    13621 gcagaaaccg tgccagcttc tgtggtgcct gtgctgccac gccacgcctg cggtgcgtgc
    13681 gacatgtggg tgcgcgttgc accacaaaca tgcgcggagc caggatatgg tgcaccaaaa
    13741 cggtgcgtcg caccagtgtg gtgcccgaaa ccggtgcagc gatcggcggc tgtgcgaggt
    13801 ggcggggttg tgccggaccc ggggtcagaa caccagggtg cgccagcgtg tgtccagcgc
    13861 ccgcgccatg aactgcaccg cgccgccctg gccgtggcat tcggggatca gaccggacgg
    13921 tgccaggcca tgggcgtgca gcgcgtcggt gcaggccagc aggcgcgcgc cgtgggcgac
    13981 cgcctcgcgc aacgcgtcca ggatggtctt gtcgtgccgc gggctggcgc gcagggcggc
    14041 ggccacgccc ggggccagca gctgcacgct ggcggcgctg aagtagattt ccaccggcac
    14101 gtccatggcg gccgcggcgg cggcgtgaaa gaacggcgtg gccagccgtt cgggctgggc
    14161 cgggtcgctg gcccacagca gaatggccac gccggcggcg ctgtgttcga cgaagctcat
    14221 ggtgcggggt ggctcgggtg gcgggtggtg ccagcgagtt gctggccatg cgccagggcg
    14281 tggcgcgcca tggccggcgc cacggcgtca ccgtgcagca gggccggctg gtcggcctgg
    14341 aagtcggcca gccgcagtcg ggcgatccag ccggtgccgt aggggtcgcg gtgcaccagt
    14401 tgggggcgat cggccagcgc cgcgttggct tcgatcacgg tgccggtgac cggcgacttg
    14461 accgccacga tggccttggc gagctccacc acggccagcg tgccgccctg tgccacctcg
    14521 gtaccgatgc gcttggcgcg gcacatgtag atctcgcccg acagccggat gcccagctcg
    14581 gtgatgccca cggtggccgt
//
LOCUS       DAOOOG010000045        14581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4429_length_14581_cov_23.360939, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000045 DAOOOG010000000
VERSION     DAOOOG010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14581
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4429_length_14581_cov_23.360939"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PK242_05290"
     CDS             <1..318
                     /locus_tag="PK242_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ93464.1"
                     /translation="AFDGQVGQQAYSASKGGVVGMTLPMARDLAQHGIRVCTIAPGLF
                     ATPLMRTLPEPVQASLAASIPFPPRLGKPSEFAELACHIVTNGHLNGEVIRLDGALRM
                     AAR"
     gene            315..728
                     /locus_tag="PK242_05295"
     CDS             315..728
                     /locus_tag="PK242_05295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOZ93465.1"
                     /translation="MSKTTTYELRVMFGDCDPAGIVFFPNFSKWMDASSLDFFMQCGV
                     PPWRELKESHGIIGTPLLEIHTKFMRPATYGDTLQVRTCVESWAAKTFVQKHQVFKGE
                     ELLCEGRETRAFCVHPEGGRLQAIAVPDFIRERCS"
     gene            775..1914
                     /locus_tag="PK242_05300"
     CDS             775..1914
                     /locus_tag="PK242_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ93466.1"
                     /translation="MKRHSFRTLVATAALAASGAALADVTIGVIAPLTGPASGLGIPV
                     GNHVKLWPKEIAGQKVNVIVMDDATDPTNGVKIAKRFVAEDKVDVIMGSVATPVAAAI
                     SQVAEESGTAQIAWSPVALPSGKDKWVFRLPQSNAVMSHAVIEHMKKQGIKSVGFLGY
                     SDAYGETWLVDFKPMAEKAGIQVGPVERFARADTSVTGQALKLVSANPQAILVVASGS
                     GAAMPEMALRERNYKGTIYQTHAAATRDLMRVGGKAVEGTFVVSGPAVLAEQLPDSHP
                     SKGEAIKYVQQYEKAYGPGSRNQFAAHAYDSVIIMEKALPIALKTAKPGTPEFRAALR
                     DAFEQLGRTVLSHGVLNWTAQDHWGYAPETGVMMKVVDGQFKVEK"
     gene            2058..2540
                     /locus_tag="PK242_05305"
     CDS             2058..2540
                     /locus_tag="PK242_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOZ93467.1"
                     /translation="MPSPTRSPAATATPTSGTEAVDRRFLQSLVGFNARMATLKVISQ
                     FIPRMAPFELRVVDFSILSLIHHNPGITSRQLCDALNLLPPNLVGKVNALQRRGLLLR
                     RPHPDDGRAQALHLTDAGLELMRRAEPTVVALENEAAAALTVTERQTLIRLLQKVYQD
                     "
     gene            2693..3001
                     /locus_tag="PK242_05310"
     CDS             2693..3001
                     /locus_tag="PK242_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ93468.1"
                     /translation="MSSKENSATSQLLALLEARYAIRVLWALRDGHPQTFRLLQDSVG
                     GITPNTLNTRLKELRAANLVTHGSDGYSLTPSGADLTKRLSDLQAFATKWVAAQTKKK
                     "
     gene            3113..3457
                     /locus_tag="PK242_05315"
     CDS             3113..3457
                     /locus_tag="PK242_05315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOZ93469.1"
                     /translation="MSTGLQTIDTLPGTGAEARKGQTVTVHYTGWLYQNGQQGAKFDS
                     SRDRNEPFRFALGAGMVIRGWDEGVAGMKVGGQRTLIIPPELGYGARGAGGVIPPHAT
                     LKFDVELLAVSN"
     gene            3637..4194
                     /gene="grpE"
                     /locus_tag="PK242_05320"
     CDS             3637..4194
                     /gene="grpE"
                     /locus_tag="PK242_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOZ93470.1"
                     /translation="MSDSLHSPPPFDPPMSPEELEAAAAAQEADAAAAQESALAELNS
                     LKAQVAALTDQALRAQAEAQNARRRADDEIAKIRKFAVESFAESLLPVIDSLEAGLKI
                     ESASTEQLREGTDATLRQLLSVLERNKVVEVNPAPGARFDPASQQAISMVAAAQEAGT
                     VVSVLQKGYTIAERVLRPALVTVAQ"
     gene            4302..6230
                     /gene="dnaK"
                     /locus_tag="PK242_05325"
     CDS             4302..6230
                     /gene="dnaK"
                     /locus_tag="PK242_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOZ93471.1"
                     /translation="MAKIIGIDLGTTNSCVSIMEGNSTRVIENAEGARTTPSIVAYQE
                     DGEVLVGASAKRQAVTNPKNTIYAAKRLIGRKFDEKEVQKDIDLMPFKIVKADNGDAW
                     VEVRGQKLAPPQISAEVLRKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKQDKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFE
                     VLATNGDTFLGGEDFDQRIIDYVIGEFKKEQGVDLKNDVLALQRLKEAAEKAKIELSS
                     TASTDLNLPYITADASGPKHLNIKLTRAKLEALVEDLIERSIAPCRTAIKDAGIAVGD
                     IQDVILVGGMTRMPKVQDKVKEFFGKEPRKDVNPDEAVAVGAAIQGQVLSGDRKDVLL
                     LDVTPLSLGIETLGGVMTKMITKNTTIPTKFAQTFSTADDNQPAVTIKVFQGEREVAS
                     GNKLLGEFNLEGIPPAPRGLPQIEVTFDIDANGILHVSAKDKGTGKENKITIKANSGL
                     SESEIQQMVKDAELNAAEDHKKVELVQARNQGDAMVHSVRKSLGEHGDKLDAGEKDKI
                     EAAIKALEEAVKGDDKADIESKTEALMTASQKLGEKMYADAAAAAQAAGAAPEGAQAG
                     AAADDNVVDAEVKEVKKD"
     gene            6350..7477
                     /gene="dnaJ"
                     /locus_tag="PK242_05330"
     CDS             6350..7477
                     /gene="dnaJ"
                     /locus_tag="PK242_05330"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOZ93472.1"
                     /translation="MAKRDYYEVLGVPKNASEDEIKKAYRKLAMKYHPDRNQGDAKSA
                     EAKFKEAKEAYEMLSDPQKKAAYDQYGHAGVDPNMRGGAEGFGGFAETFGDIFGDIFG
                     GGRGGARGGRQVYRGADLSYAMDITLEEAAAGKEAQIRIPSWDDCDTCHGSGAKPGTS
                     ASTCTTCHGQGVVQMRQGFFSVQQTCPHCRGTGKVIKDPCTSCHGQGKVKNQKTLEVK
                     IPAGIDDGMRIRSAGNGEPGSNGGPPGDLFIEIRLKKHDIFERDGDDLHCVVPIGFAT
                     AALGGEIEVPTLSGKAAIDIPEGTQAGKQFRLRGKGIKGVRSSYPGDLYCHISIETPV
                     KLTEHQRKLLKEFDESLRKGGAKHSPGTESWTDRLKSFFGA"
     gene            complement(7604..8074)
                     /locus_tag="PK242_05335"
     CDS             complement(7604..8074)
                     /locus_tag="PK242_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOZ93473.1"
                     /translation="MSAPDFDLQRDAFGRLVLTDAQGHAHVGVAAVRAFPIGAPDEGI
                     SLVDGDGHELAWVPRLGALDEDRRALLVEALEQREFMPRLLRVRGVSSFVTPSTWEVD
                     TDRGPTRFVLKGEEDIRRLGPGLLIVNDAHGVQYLIQDLMAMDRHSRKLLDRFL"
     gene            complement(8071..10383)
                     /locus_tag="PK242_05340"
     CDS             complement(8071..10383)
                     /locus_tag="PK242_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ93474.1"
                     /translation="MPAGDPAPAIEEAPPLRPTENVLGSLDVDLTRQLRFGRGVILIT
                     DQRLVTRFPDATGWQAWDLHPGLSLAHHDHAGVGTLELHDAQGRLAVWRYTLGHNPAA
                     LRLLGQFERAREALAGGAPSASEDVAPADQAEDEEGVPDQPPSTWTLLRLWRFARPYQ
                     WQLLAGFVLMLAATAATLVPPYLTMPLMDKVLIPFQSGQQIDTGYVALLLTGLLGSAL
                     LAWGLGWAKTYILALVSERIGADLRTITYEHLLKLSLEYFGGRRTGDLMARIGSETDR
                     INIFLSLHLLDFATDVLMIAMTAAILFSIDPTLALVTLVPLPIIAWLIHVVRDRLRTG
                     FEKIDRVWSEVTNVLADTIPGIRVVKAFAQEDREASRFRDANRHNLAVNDRLNRLWSL
                     FSPTVTLLTEIGLLIVWAAGIWLVSQQRVTVGVLTAFLAYIGRFYTRLDSMSRIVSVT
                     QKAASATKRIFEILDHVSSVPELKNPVPLPRVQGRIDIRGAALRYGNRSVLRGLDLHI
                     EPGQMIGLVGHSGSGKSTLVNLICRFYDVSEGAIEVDGVDLRQVRVADWRRHIGLVLQ
                     EPYLFFGTIAENIAYGKPDATREEIVAAARAAHAHEFILRLPQGYDSLVGERGQGLSG
                     GERQRISIARALLIDPKILILDEATASVDTETEREIQKALDNLVRGRTTIAIAHRLST
                     LRKADRLVVMDKGVKVEEGTHEVLLARRGAYWRLYEAQARQEKAERERMLVVDDAVLA
                     GEPTAVPAIATPTHRGDVSTAEARRQESQP"
     gene            10610..12733
                     /gene="cphA"
                     /locus_tag="PK242_05345"
                     /pseudo
     CDS             10610..12733
                     /gene="cphA"
                     /locus_tag="PK242_05345"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
     gene            12817..>14581
                     /gene="cphA"
                     /locus_tag="PK242_05350"
     CDS             12817..>14581
                     /gene="cphA"
                     /locus_tag="PK242_05350"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOZ93475.1"
                     /translation="MEITRIRALRGPNLWSRHTAIEAVVTCDAAERDLHQQPTFEQRL
                     RELFPHVGTLQVNQPAAPSTPLSMAHALELVTLALQAQAGCPVTFSRTTPTEIEGTYQ
                     VAVQYTEEAVGRLAFDKAQLLCRAAAEGGQFDVGAALAELRELDEDERLGPSTGAIVD
                     AAVRRGIPFKRLTRGSLVQLGWGSRQRRIQAAEVDGTSAIAESIAQDKDLTKKLLHAA
                     GVPVPLGRPVSDLQDAWAAAQEIGLPVVVKPQDGNQGKGVTVGILDREHFDIAYQAAA
                     QYGTVMVERFLPGHDFRLLVVGNQLVAAARREPPLVVGDGEHTVRELVAEVNKDPRRG
                     EGHGTSLTRIRIDAIAEARLAAQGLTPDSVPTKGQRVVMRNNANLSTGGTATDVTDSV
                     HPLVAARAIEAAQTVGLDICGVDVVCETVIKPLEEQNGGIVEVNAAPGLRMHLSPSYG
                     KGRDVGQAVIDHMFPDGGNGRIPVIAVTGTNGKTTTVRLAAHLLKTSGLRVGMTNTDG
                     VYVNGRQIDSGDCSGPRSARNVLAHPDVDAAVLETARGGMLREGLAFDQCDVAIVTNL
                     GGGDHLGLNYITTLEDLKVLKR"
BASE COUNT         2668 a         5128 c         4723 g         2062 t
ORIGIN      
        1 gccttcgacg gccaggtggg ccagcaggcc tacagcgcca gcaagggcgg cgtggtcggc
       61 atgacgctgc cgatggcgcg cgatctggcg cagcacggca tccgggtgtg caccatcgcc
      121 ccgggcctgt ttgccacgcc gctgatgcgc accctgcccg agccggtgca ggcctcgctg
      181 gccgcgtcca tccccttccc gccgcgcctg ggcaagccca gcgaatttgc cgagctggcc
      241 tgccacatcg tcaccaacgg gcacctcaac ggcgaggtga tccgcctgga cggcgcgctc
      301 cggatggccg cacgatgagc aagaccacca cctacgagct cagggtgatg ttcggcgact
      361 gcgatccggc cggcatcgtt ttctttccca acttcagcaa gtggatggac gcttcgtcgc
      421 tcgacttctt catgcagtgc ggcgtgccgc cctggcgcga actgaaggaa agccacggca
      481 tcatcggcac gcccctgctg gaaatccaca ccaaattcat gcgccccgcc acctacggcg
      541 acacgctgca ggtgcgcacc tgcgtcgagt cctgggctgc caagaccttt gtgcaaaagc
      601 accaggtgtt caagggcgag gagctgctgt gcgaaggccg cgagacgcgg gccttttgcg
      661 tccaccccga gggcggacgg ctgcaggcga tagctgtgcc cgatttcatt cgcgagcgct
      721 gttcgtgatg tgtccgtgcc gagctttttc cacccaaccc caaggagaca aaccatgaaa
      781 cgccattcct tcagaaccct ggtggccacc gccgccctgg ccgccagcgg cgcggcgctg
      841 gccgatgtca ccatcggggt gatcgccccg ctcaccgggc cggcttcggg cctgggcatt
      901 ccggtgggca accacgtcaa gctctggccc aaggagatcg ccggccagaa ggtcaacgtg
      961 atcgtgatgg acgacgccac cgacccgacc aatggcgtca aaatcgccaa gcgcttcgtg
     1021 gccgaagaca aggtggatgt gatcatgggc tcggtcgcca cgcccgtggc cgcggccatc
     1081 agtcaggtgg ccgaggaatc gggcaccgcg cagatcgcct ggtcgccggt ggcgctgcct
     1141 tcgggcaagg acaaatgggt gttccgcctg ccgcagtcca acgccgtgat gagccatgcg
     1201 gtgatcgagc acatgaagaa gcaaggcatc aagtcggtgg gctttctggg ctacagcgac
     1261 gcctatggcg aaacctggct ggtcgacttc aagcccatgg ccgaaaaagc cggcatccag
     1321 gtcggcccgg tggagcgctt tgcccgcgcc gacaccagcg tgaccgggca ggcgctcaag
     1381 ctcgtgtcgg ccaaccccca ggccatcctg gtggtggcct cgggcagcgg cgccgccatg
     1441 cccgaaatgg cgctgcgtga gcgcaactac aagggcacga tctaccagac ccatgccgcc
     1501 gccacacgtg acctgatgcg cgtgggcggc aaggcggtgg aaggcacctt cgtggtctcc
     1561 ggcccggcgg tgctggccga acagttgccc gactctcacc cgtccaaggg cgaggccatc
     1621 aagtacgtgc agcagtacga aaaggcctat ggtcccggct cgcgcaacca gtttgccgcc
     1681 cacgcctacg actcggtcat catcatggag aaggcgctgc cgatcgccct gaagaccgcc
     1741 aagcccggca cgcccgaatt ccgcgccgcg ctgcgcgacg ccttcgagca gctgggccgc
     1801 accgtgctct cgcacggcgt gctcaactgg accgcgcagg accactgggg ctacgcgcct
     1861 gaaaccggcg tgatgatgaa ggtggtggac ggtcagttca aggtcgaaaa gtaacgaccg
     1921 cgcggcaccc accccacggg gccgcgggca gaccgcagcg ccagggcttg ttgcagcgct
     1981 tgccggaggg cgccgtcgtg tgcgccgccc gcaagcgctg tgcgatcatc cgccctgccc
     2041 ccagccgccc gcgccccgtg ccttcgccca cccgctcccc cgccgccacc gcaacgccca
     2101 cctccggcac cgaggcggtg gatcggcgct ttctgcagtc gctggtcggc ttcaacgccc
     2161 gcatggcgac gctcaaggtg atcagccagt tcattccgcg catggcgccc tttgagctgc
     2221 gggtggtcga tttttcgata ctgtcgctga tccaccacaa ccccggcatc acctcgcgtc
     2281 agctgtgcga cgcgctcaat ctgctgcccc ccaacctggt gggcaaggtc aacgccctgc
     2341 aacgacgcgg cctgttgctg cgccgacccc acccggacga cggccgcgcc caggccttgc
     2401 acctgaccga cgcaggcctt gagctgatgc ggcgcgccga acccaccgtg gtggcgctgg
     2461 agaacgaagc cgccgccgcc ctgacggtga ccgaaaggca gaccctgatc cggctgctgc
     2521 agaaggtcta tcaagactaa gggttttccc gggccgacgc ttcggtcgat tccactattt
     2581 gtagcgccac atccacggcc cggcagtgcc gggagctatg aaactgctag cgtgctacag
     2641 atttgcgata tagtcccctc acgcaccgag cgaacccgag gagaaccccg acatgagttc
     2701 caaagaaaat tccgccacca gtcagctgct ggcgctgctt gaagcgcgat acgccattcg
     2761 tgttctgtgg gccctgcgcg acggccatcc gcaaaccttc cgcctgctgc aagacagcgt
     2821 cggcggcatc acgcccaaca ccctcaacac ccgcctgaag gagctgcgcg cggccaacct
     2881 ggtcacccac ggcagcgacg gctacagcct cacccccagc ggcgccgatc tgaccaagcg
     2941 cctgtccgac ctgcaggcct tcgccaccaa gtgggtggcg gcacagacca agaagaagtg
     3001 aggccgcggc ggcgcgctcg gggcttcctc cggccaaccc ggttcttcta accaaatccg
     3061 gccccagcgc ttatctggca ggcgccacaa gctatcaaaa ttatagtatt tcatgagcac
     3121 cggactgcaa accatcgaca ccctgcccgg caccggcgcc gaggcccgca agggccagac
     3181 cgtcaccgtg cactacaccg gctggctcta ccagaacggg cagcagggtg ccaagttcga
     3241 ctccagccgc gaccgcaacg aaccgttccg cttcgcgctc ggtgccggca tggtgatccg
     3301 tggctgggac gaaggcgtgg cgggcatgaa ggtcggcggc caacgcaccc tgatcatccc
     3361 gcccgaactg ggctacggcg cccgcggcgc cggcggcgtg atcccgcctc atgcgacgct
     3421 gaagttcgac gtcgagttgc tcgccgtctc gaactgagcc tgggccaccc cctgcaacgc
     3481 cgcctgcggg cggcggtttc cgttctggaa cccatggcag ccaagtggcg ctccccagat
     3541 cgccggtttt cgggccgccc tgcccttgaa aacccgcgtt ccggggccat ctgagggggg
     3601 tttgttccgt ctgttcgcct tttttcacca gcctgcatgt ccgactcgct ccattcccca
     3661 cccccattcg accctcccat gagccccgaa gagctggagg cggccgccgc cgcccaggag
     3721 gccgatgccg ccgccgccca ggaaagcgca ctggccgagc tgaacagcct gaaagcgcag
     3781 gtcgccgccc tcaccgacca ggcgctgcgt gcccaggccg aggcccagaa cgcccgccgg
     3841 cgcgccgacg atgagatcgc caagatccgc aagttcgccg tcgagtcctt cgccgaaagc
     3901 ctgctgccgg tgatcgacag cctggaggct ggcctgaaga tcgaaagcgc cagcaccgaa
     3961 cagctgcgtg aaggcaccga cgccaccctg cggcagctgc tcagcgtgct ggagcgcaac
     4021 aaggtggtcg aagtcaaccc cgcacccggc gcccgtttcg accctgccag ccagcaggcc
     4081 atcagcatgg tggcggccgc gcaggaggcc ggcaccgtcg tcagcgtgct gcaaaagggc
     4141 tacaccatcg ccgaacgcgt gcttcggcca gccctggtca ccgtggccca gtaatcgacc
     4201 caccgtctga tgcgcgggcc tggcgacgct gcttgaattt cgcgcgccgg ccctccacct
     4261 gaaccacaac ctcattcatc tacccctcgc ggagaaccat catggcaaaa atcatcggca
     4321 tcgacctggg caccaccaac agctgcgtgt ccatcatgga aggcaactcg acccgcgtga
     4381 tcgagaacgc cgaaggcgcg cgcaccacgc cgtccatcgt cgcctaccag gaagacggtg
     4441 aggtgctggt gggcgcctcg gccaagcgcc aggccgtcac caaccccaag aacaccatct
     4501 acgccgccaa gcgcctgatc ggccgcaagt tcgacgaaaa ggaagtgcag aaggacatcg
     4561 acctgatgcc cttcaagatc gtcaaggccg acaacggcga cgcctgggtc gaagtgcgcg
     4621 gccagaagct ggcgccgccg cagatcagcg ccgaggtgct gcgcaagatg aagaagaccg
     4681 ccgaggacta cctgggcgag cccgtcaccg aggccgtcat caccgtcccc gcctacttca
     4741 acgacagcca gcgccaggcc accaaggacg ccggccgcat cgccggcctg gatgtcaagc
     4801 gcatcatcaa cgagcccacc gccgcggccc tggccttcgg cctggacaag caggacaagg
     4861 gcgaccgcaa gatcgccgtc tatgacctcg gtggcggcac gttcgacgtc tccatcatcg
     4921 agatcgccga cgtcgacggc gaaaagcagt tcgaagtgct ggccaccaac ggtgacacct
     4981 tcctgggcgg cgaagacttc gaccagcgca tcatcgacta cgtgatcggc gagttcaaga
     5041 aagagcaagg ggtcgatctg aagaacgacg tgctggccct gcagcgcctg aaggaagcgg
     5101 ccgaaaaggc caagatcgag ctgtcgagca ccgcctcgac cgacctcaac ctgccctaca
     5161 tcacggccga cgccagcggg cccaagcacc tgaacatcaa gctcacgcgc gccaagctgg
     5221 aagccctggt cgaagacctg atcgagcgct cgatcgcgcc ctgccgcacc gccatcaagg
     5281 acgccggcat cgccgtgggc gacatccagg acgtgatcct ggtgggcggc atgacgcgca
     5341 tgcccaaggt gcaggacaag gtcaaggagt tcttcggcaa ggagccgcgc aaggacgtca
     5401 accccgacga ggcggtggcc gtgggcgccg ccatccaggg ccaggtgctg tcgggcgacc
     5461 gcaaggacgt gctgctgctg gacgtgacgc cgctgtcgct gggcatcgaa accctgggcg
     5521 gcgtgatgac caagatgatc accaagaaca ccaccatccc gaccaagttc gcccagacct
     5581 tctccaccgc cgacgacaac cagccggccg tgaccatcaa ggtgttccag ggtgagcgcg
     5641 aggtggccag cggcaacaag ctgctgggcg aattcaacct ggaaggcatt ccgcccgcac
     5701 cgcgcggcct gccgcagatc gaagtgacct tcgacatcga cgccaacggc atcctgcacg
     5761 tcagcgccaa ggacaagggc acgggcaagg aaaacaagat caccatcaag gccaactcgg
     5821 gcctgtccga gtccgagatc cagcagatgg tcaaggacgc cgagctgaat gccgccgaag
     5881 accacaagaa agtcgagctg gtacaggcgc gcaaccaggg cgacgccatg gtgcacagcg
     5941 tgcgcaagtc gctcggtgag cacggcgaca agctcgacgc cggcgagaag gacaagatcg
     6001 aggccgccat caaggccctg gaagaagccg tcaagggcga cgacaaggcc gacatcgaaa
     6061 gcaagaccga ggccctcatg acggccagcc agaagctggg cgagaagatg tatgccgacg
     6121 ccgcagccgc cgcccaggcc gccggcgctg ccccggaagg ggcgcaagcc ggcgcggccg
     6181 ccgacgacaa cgtggtcgac gccgaggtca aggaagtcaa gaaggactga cgcccatgaa
     6241 cgcgccgccg gccagcgcaa aggccggcgc gcgcccgccg cgcagaggag gtctggcaac
     6301 agacaccacc ctgcgcggcg ttgttgcttg agagatggaa agaacgcaga tggccaagcg
     6361 cgattactac gaagtcctgg gtgttcccaa gaacgcgtcc gaggacgaga tcaagaaggc
     6421 ttatcgcaag ctggcgatga agtaccaccc ggaccgcaat cagggtgacg ccaagagcgc
     6481 cgaggccaag ttcaaggagg ccaaggaagc ctacgaaatg ctgtccgacc cgcagaagaa
     6541 ggccgcctac gaccagtacg gccatgcggg ggtggacccc aacatgcgcg gcggagcaga
     6601 aggcttcggc ggctttgccg agacctttgg cgacatcttc ggcgacatct tcggtggcgg
     6661 gcgcggtggc gcgcgcggtg gccggcaggt gtaccgcggc gccgatctca gctacgcgat
     6721 ggacatcacg ctggaagagg ctgccgccgg caaggaagcg cagatccgca tcccgagctg
     6781 ggacgactgc gacacctgcc acggctccgg cgccaagccc ggcaccagcg ccagcacctg
     6841 caccacctgc cacggccagg gcgtggtgca gatgcgccag ggttttttca gtgtgcagca
     6901 gacctgcccg cactgccgcg gcaccggcaa ggtcatcaag gacccctgca ccagttgcca
     6961 cggccagggc aaggtcaaga accagaagac cctggaggtg aagatccccg ccggcatcga
     7021 cgacggcatg cgcatccgct cggccggcaa cggtgagccg ggcagcaacg gcgggccgcc
     7081 gggcgatctg ttcatcgaga tccgcctgaa gaaacacgac atcttcgagc gcgacggcga
     7141 cgacctgcac tgcgtggtgc cgatcggttt cgccaccgcg gcactgggcg gcgagatcga
     7201 ggtgcccacg ctcagcggca aggcggcgat cgacattccc gaaggcacgc aggcgggcaa
     7261 gcagtttcgc ctgcgcggca agggcatcaa gggcgtgcgc tcgtcctacc cgggcgacct
     7321 gtactgccac atcagcatcg aaacgccggt caagctgacc gagcaccagc gcaagctgct
     7381 gaaggagttc gacgaatcgc tgcgaaaagg cggcgccaag cactcacccg gcaccgaaag
     7441 ctggaccgac cgcctcaaga gcttcttcgg cgcctgaccg gcgccccggc gcgaccgccc
     7501 cgctcaagga cgtcgcaaca ccgctgccgc cccaacgatt tcaagtcaaa acgacctctg
     7561 gcccatgcct ggcaagcgcg agcagctata tttgttgtag cgttcagagg aagcggtcga
     7621 gcaacttccg gctgtggcgg tccatggcca tcagatcctg gatcaggtac tgcacgccat
     7681 gggcgtcgtt gacgatcagc aggccgggcc cgagacggcg gatgtcttcc tcacccttga
     7741 gcacgaagcg ggtcggaccg cggtcggtgt cgacctccca ggtgctcggc gtcacgaaac
     7801 tgctcacgcc cctcacgcgc agcagacgcg gcatgaactc gcgctgctcc agcgcctcga
     7861 ccagcagggc gcgacggtcc tcgtccaatg cgccgagccg cggcacccag gccagttcat
     7921 gaccgtcgcc atcgaccagg ctgatccctt cgtcgggcgc accgatcgga aacgcccgca
     7981 cggccgccac gccgacatgc gcatgcccct gggcgtcggt cagcaccagg cgaccaaagg
     8041 cgtcgcgctg cagatcgaag tcgggcgcgc tcatggctgg gactcctggc gccgagcctc
     8101 ggccgtgctg acgtcgccgc ggtgcgtcgg cgtggcaatg gcgggcaccg ccgtcggctc
     8161 accggccagc acggcatcgt cgaccaccag catgcgctcg cgctcggcct tctcctgccg
     8221 cgcctgcgcc tcgtacaggc gccagtaggc gccgcggcgc gccagcagca cttcgtgcgt
     8281 gccctcctcg accttcacgc ccttgtccat caccaccagc cggtcggcct tgcgcagcgt
     8341 ggacaggcgg tgcgcgatgg caatcgtcgt gcgcccacgc accaggttgt ccagcgcctt
     8401 ctggatctcg cgttcggttt cggtgtcgac cgaggccgtg gcctcatcca ggatcaggat
     8461 cttggggtcg atcagcagcg cgcgggcgat gctgatgcgc tggcgctcgc cgcccgacag
     8521 cccctggccg cgctcgccca ccagcgagtc gtagccctgc ggcagccgca ggatgaactc
     8581 gtgcgcgtgg gcggcgcgcg cggcggccac gatctcctcg cgcgtggcgt cgggcttgcc
     8641 gtaggcgatg ttctcggcga tggtgccgaa aaacaggtag ggctcctgca gcaccaggcc
     8701 gatgtggcgg cgccagtcgg ccacccgcac ctgccgcagg tccacgccat cgacttcgat
     8761 ggcgccctcg ctcacgtcat agaagcggca gatcaggttc accagcgtgc tcttgccgga
     8821 gccgctgtgg cccaccaggc cgatcatctg acccggttcg atgtgcaggt ccaggccgcg
     8881 cagcaccgag cggttgccgt agcgcagcgc cgcaccgcgg atgtcgatgc ggccctgcac
     8941 gcgtggcagc ggcacggggt ttttcagctc cggcacgctg gagacatggt cgaggatttc
     9001 aaagatgcgc ttggtggccg aggccgcctt ctgcgtcacc gagacgatgc ggctcatcga
     9061 atcgagccgg gtgtagaagc gcccgatgta agccagaaac gccgtcagca cccccaccgt
     9121 aacgcgctgc tggctgacca gccagatgcc ggcggcccag acgatcagca gcccgatttc
     9181 ggtcagcaac gtgacggtgg gcgaaaacag cgaccacagg cggttcaggc ggtcgttgac
     9241 ggccaggttg tggcggttgg catcgcgaaa gcgactcgct tcgcggtctt cctgggcgaa
     9301 ggccttgacc acgcggatac ccggaatggt gtcggccagc acgttggtga cttccgacca
     9361 gacgcggtcg atcttctcga agccggtgcg caggcggtcg cgcaccacgt ggatcagcca
     9421 cgcaatgatc ggcagcggca ccagggtcac cagcgccagc gtcgggtcga tggaaaacag
     9481 gatcgccgcc gtcatggcga tcatcagcac gtcggtggcg aagtccagca ggtgcagcga
     9541 cagaaaaatg ttgatgcggt cggtttccga accgatgcgc gccatcaggt ctcccgtgcg
     9601 ccggccaccg aaatattcaa gtgaaagttt gagcaggtgt tcgtaagtga ttgtccgcag
     9661 gtcggcaccg atgcgctcgg acaccagcgc caggatgtag gtcttggccc agcccaggcc
     9721 ccacgccagc aaggccgacc ccagcaggcc ggtcagcagc agcgccacgt agccggtgtc
     9781 gatctgctgg ccgctctgga acgggatcag caccttgtcc atcagcggca tggtcaggta
     9841 gggcggcacc agggtggcgg cggtggccgc cagcatcagc acgaaaccgg ccagcagctg
     9901 ccactggtag ggcctggcaa agcgccacag gcgcagcagg gtccaggtgg acggcggctg
     9961 gtccggcaca ccctcttcat cctcggcctg gtcggccggg gcgacgtctt cgctggccga
    10021 cggcgcgccg ccggccagcg cctcacgggc gcgctcgaac tgccccagca ggcgcagcgc
    10081 cgccgggttg tgccccaggg tgtagcgcca gaccgccagc cttccctgcg cgtcgtgcag
    10141 ctccagcgtg cccaccccgg cgtggtcgtg gtgcgccagc gacagcccgg ggtgcaagtc
    10201 ccaggcctgc cagccggttg catccggaaa ccgcgtgaca agccgttgat ctgtaatgag
    10261 aatcaccccg cgcccaaagc gcagctggcg cgtcaggtca acgtccagcg agcccagcac
    10321 gttttctgtg ggtcgcaggg gtggcgcctc ttcgatggca ggcgcggggt cacccgccgg
    10381 catggccagg cggggatcgg acgacgtggg ttccggcaag gcagcccttc agacagcaac
    10441 gaaacacgga aagcgagcgc agggccccat gaggcgaatc gatcaacacg aacacggctc
    10501 ggcaaacggt ggcggattgt atgtccgcgc cccacaagcc gccgccgaac tggcgccggc
    10561 gtcggccgca ccgcccgatc gccagttcgc cgacatcatc atccgccgca tggcgcatct
    10621 gcgcggcccc aacatctgga cctaccgcgc ggtgatcgag gccatcgtcg acatcggcga
    10681 actggaagac ttcccctcca acaccctgcc cggcttttac gaccgcctga cggcctggct
    10741 gcccggtctg atcgagcacc gctgcagcgt cggccgtcgc gggggctttc tgatgcgcct
    10801 gcgcgacggc acctggccgg gccacatcct ggagcacgtg gcgctcgaac tgcagaccca
    10861 ggccggcatg aagaccggct tcggcaaggc ccgcatgacg cacgagcgtg gcgtctacaa
    10921 ggtggtcatc cgcacccgtc acgaagccgt cggcaggctg gccatcacct cggcgcgcga
    10981 tctggtgatg gcggccatca acgaccgccc ctacgacgtc gccgccacca tcgcccgact
    11041 gaccgagatg gtcgatcgcc agtgcctggg gcccagcacg gcctgcatcg tcgacgccgc
    11101 ggtggaacgc ggcattccca acatccggct caacgacggc aacctggtgc agctcggcca
    11161 cggcgccgcg caacaccgca tctggaccgc cgaaaccgac cgcaccagcg ccatcgccga
    11221 aggcattgcg agcgacaagg acctgaccaa gcagctgctg gcggccgccg gcgtgccggt
    11281 gcccgagggc cgcaccgtca gcagtgccga cgacgcctgg gacgccgccg aggacatcgg
    11341 cctgcccgtg gccgtcaagc cgtcggacgg caaccatgcg cgcggcgtgt cgctgaacct
    11401 caagacacgc gacgaggtcg tgcgcgcctt cgccgccgcc gaacaggaag gctccgaagt
    11461 gatcgtcgag cgcttcattc ccggccagga gcaccggctg ctggtggtcg gtggcaaggt
    11521 ggtggcggcc acgcgtggcg aaatcatccg cgtcagcgga gatggtcgct ccagcatcgg
    11581 ccagctcatc gacgcccacg tcaacaccga cccgcggcgc ggcgtcgaag aacatctgcc
    11641 gctggaaacc ctgcgccacg acgacccggt gctgctgctg ctgctggagc gccagggcct
    11701 gggcatcgac agcgtgctgc cgcgcggcga atcggcggag gtgcagcgca ccggcaacat
    11761 gtcgatcgac gtcaccgggc aggtgcaccc cgacgtggcc gagctggtgg caatggccac
    11821 ccgcgtggtc gggctggaca tcgccggcat cgacctggtg gccgaagaca tcggccagcc
    11881 gctgattccg cagggcggcg cgatcgtcga agtcaacgcc gggcccggcc tgctgatgca
    11941 cctcaaaccg gccatcggcc gcgcccagcc ggtgggtgac gccatcgtca accatctgtt
    12001 ccccgccgga gccagcgggc gcattccgat cgtcggcatc atcggcgacg gcgaaagcgc
    12061 gctggcggcg cgcctgaccg ccgccctgat gcagttgcac ggcctgcaga cggcactggg
    12121 ctgccgagat ggcctgttcc tcggccagcg ccggctggcg agcggcggtg cgctgggcta
    12181 cgacatcggt gagcggatgc tcatcaaccg cacagtgcag gccgccgtgt tcgaaacctc
    12241 gccgcgccac atcctgaccg aaggcctgcc ctacgaccgc tgccaggtcg gtgtggtcat
    12301 gtgcatgccc gggccgcagg gcctggagga tctgtacatc accgaggccg accagatgcc
    12361 caacatcgtg cgcacccagg tcgacgtggt gctgcccgaa ggggcggcgg tgctgaacgc
    12421 cgacgacgcc gaagtgctgg cgctggccga ctacgccgac ggcgaagtgc tgctctacag
    12481 cctcgacccg cagagcccgg ccttgaaggc gcaccgcgcg cgcaagggcc gcgtcgccct
    12541 ggcccgcggc gatcgcatcg tgctggcgca aggcccggag gagtttgcgc tggtcaacct
    12601 gtcggcgccc gagttttccg ccatcgccca ggaaccgccg gccgacgtgc gcccgcatgt
    12661 gctggcggcc gtcgccacgg cctggtcgct gggcgtgccg gtggagctga tccgtgccgg
    12721 tttgttgcac ttttgtgaag cggcaccggc cggcgctgtc cactgagcca ccggctcagc
    12781 cgttgcgtag atcagcccat tccttccagg accatcatgg aaatcacccg cattcgcgcg
    12841 ctgcgcggcc ccaacctgtg gagccgccac acggccatcg aggccgtggt cacctgcgac
    12901 gcagccgagc gcgacctgca ccaacagccc accttcgaac aacgcctgcg tgagctgttt
    12961 ccccatgtag gcacgcttca ggtcaaccag cccgcggcgc ccagcacgcc cctgtccatg
    13021 gcccacgcgc tggagctggt gaccctggcc ctgcaggctc aggcgggttg cccggtcacg
    13081 ttcagccgca ccacaccgac cgaaatcgaa ggcacgtacc aggttgccgt ccagtacacc
    13141 gaagaagccg tgggccgcct ggccttcgac aaggcccagc tgctgtgccg cgccgcagct
    13201 gaaggcggcc agttcgatgt cggcgccgcc ctggccgaac tgcgcgaact cgacgaagac
    13261 gagcgcctgg gcccttcgac cggcgccatc gtcgacgccg ccgtgcggcg tggcatcccg
    13321 ttcaagcggc tgacgcgcgg cagtctggtg cagctgggct ggggcagtcg ccagcggcgc
    13381 atccaggcgg ccgaagtcga cggcaccagc gcgattgccg aatcgattgc ccaggacaag
    13441 gacctgacca aaaagctgct gcacgccgcc ggcgtgcccg tgcccctggg ccgcccggtc
    13501 agcgatctgc aggacgcctg ggcagccgcg caggagatcg gcctgccggt ggtggtcaaa
    13561 ccccaggatg gcaaccaggg caagggagtg accgtcggca tccttgatcg cgagcacttc
    13621 gacatcgcct accaggccgc ggcccagtac ggcacggtga tggtcgagcg ctttctgccc
    13681 ggccatgatt tccggctgct ggtggtaggc aaccagttgg tggcggcagc gcgccgcgag
    13741 ccgccgctgg tggtcggtga cggtgaacac acggtgcgcg aactggtggc cgaagtcaac
    13801 aaggatccgc gccgtggcga aggtcacggc acctcgctga ccaggatccg catcgacgcc
    13861 atcgccgagg ctcgcctggc ggcccagggc ctgacgcccg acagcgtgcc caccaaaggt
    13921 cagcgcgtgg tgatgcgcaa caacgccaac ctgtccaccg gcggcaccgc caccgacgtg
    13981 accgacagcg tccaccccct ggtggcggcg cgggccatcg aggccgcaca gaccgtgggc
    14041 ctggacatct gcggcgtcga cgtggtgtgc gagacggtca tcaagccact ggaagagcag
    14101 aacggcggca tcgtcgaagt caacgccgcg cccggcctgc gcatgcacct gagcccgtcc
    14161 tacggcaagg gccgcgacgt cggccaggcg gtgatcgacc acatgttccc tgacggtggc
    14221 aatggccgca tcccggtgat cgccgtcacc ggcaccaacg gcaagaccac caccgtgcgc
    14281 ctggccgcgc acctgctcaa gaccagcggt ctgcgggtgg gcatgaccaa caccgacggc
    14341 gtctacgtca atggccggca gatcgactcc ggcgactgct ccggaccgcg cagcgcgcgc
    14401 aacgtgctgg cccaccccga tgtcgatgcc gccgtgctgg aaaccgcgcg cggcggcatg
    14461 ctgcgcgaag gcctggcctt cgatcagtgc gacgtcgcca tcgtcaccaa cctgggcggc
    14521 ggcgatcacc tgggcctgaa ctacatcacc acgctggaag acctgaaggt gctcaagcgc
    14581 g
//
LOCUS       DAOOOG010000046        14574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4434_length_14574_cov_23.629107, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000046 DAOOOG010000000
VERSION     DAOOOG010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14574
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4434_length_14574_cov_23.629107"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PK242_05355"
     CDS             complement(<1..920)
                     /locus_tag="PK242_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOZ93476.1"
                     /translation="MTTIIKQGDLIESIAAALQYISYYHPADYIQHLARAYEREQSPA
                     AKDAMAQILTNSKMSATGHRPICQDTGIVNVFLKIGMNVRFEGFAADQSLEDAVNEGV
                     RRGYNHPDNTLRASVVADPLFARKNTRDNTPAVINVQIVPGDKLDVTVAAKGGGSENK
                     SKMVMMNPSDNLVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAVLLAKESLMEDLNM
                     YELQAKAARGEKLDQVEELRLELYEKVNALGIGAQGLGGLSTVLDVKIKMYPTHAASK
                     PVAMIPNCAATRHAHFVMDGSGPVYLEAPSL"
     gene            1033..2427
                     /gene="fumC"
                     /locus_tag="PK242_05360"
     CDS             1033..2427
                     /gene="fumC"
                     /locus_tag="PK242_05360"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOZ93477.1"
                     /translation="MSDADKTTRVERDSFGPIEVPAERLWGAQTQRSLRHFAISGERQ
                     PRELIHALTQVKRAAAVVNQRLGQLDATLAGAIVRAADEVLAGRHDDEFPLVVWQTGS
                     GTQTNMNLNEVLANRASELLGGPRGEQRLVHPNDHVNRSQSSNDAFPTAMHVAAAEAI
                     ERRLLPALTRLRATLAGKSRDFADIVKIGRTHLQDATPLTLGQELSGWVAQLAHAGRH
                     VRAALPHLHELALGGTAVGTGLNAPEGYAEAVAQELARLTGLPFVTAPNKFEALASCD
                     ALVQAHGMLKGLAASLTKIANDVRWLASGPRSGLGEISIPENEPGSSIMPGKVNPTQC
                     EALTMLCAQVMGNDVAISIGGASGHFELNVFRPMIAHAFLQSARLLADGMNSFNDHCA
                     VGIEPNRARIDELVQRSLMLVTALNPHIGYDKAAQIAKRAHQEGLSLREAALATGWLS
                     AAQFDAWVRPQDMV"
     gene            2652..4634
                     /gene="acs"
                     /locus_tag="PK242_05365"
     CDS             2652..4634
                     /gene="acs"
                     /locus_tag="PK242_05365"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOZ93478.1"
                     /translation="MSAIQSVLVENRVFPPSEAMVKAARISGMGAYQALYEEAEKDFE
                     GFWARRAREHLVWAKPFTQVLDESNKPFYKWFADGQLNASANCLDKHMGTPVENKTAI
                     IFEADGGEVTKVTYKELLARVSQFANGLKAHGIRKGDRVLVYMPMTIEGLIAMQACAR
                     IGATHSVVFGGFSAKAVQERIQDAGAVAVITANYQMRGGKELPLKAIVDEALSLGGCE
                     SIKNVFVYERTQTPCNMVAGRDKTFAQMLQGQSTECAPEIVDAEHPLFVLYTSGSTGK
                     PKGVQHSTGGYLLWAKMTMEWTFDLKDSDVFWCTADIGWVTGHTYIVYGPLAAGATQI
                     MFEGVPTYPDAGRFWQMIERHQCSIFYTAPTAIRSLIKASETNDKVHPRNWNLSSLRI
                     LGSVGEPINPEAWMWYYRNVGGERCPIVDTWWQTENGGHLITPLPGATPLVPGSCTLP
                     LPGVFAGIVDEAGNDMPWGSGGILVIKKPWPSMIRTIWGDPARFKSSYFPEELKGYYL
                     AGDGAVRDAERGYFRVTGRIDDVLNVSGHRMGTMEIESALVAKTDLVAEAAVVGRPDE
                     TTGEAICAFVVLKRPLPQGDEAKQIAKELRDWVAKEIGPIAKPKDIRFGENLPKTRSG
                     KIMRRLLRSLAKGEQITQDTTTLENPAILAQLDQTY"
     gene            complement(4739..5050)
                     /locus_tag="PK242_05370"
     CDS             complement(4739..5050)
                     /locus_tag="PK242_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ93479.1"
                     /translation="MKRVLLTFAALTAVTVSAPALADEAMAKAKNCMACHATDKKLVG
                     PSYKDVAKKYASDAKAVDTLAAKIIKGGSGVWGAVPMPPNANVNDAEAKKLAAWVMSL
                     K"
     gene            5158..5412
                     /locus_tag="PK242_05375"
     CDS             5158..5412
                     /locus_tag="PK242_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04438 family Trp-rich protein"
                     /protein_id="HOZ93480.1"
                     /translation="MYLLGLGIILLLMKWQELGPVAAWSWWWVLSPFAGAALWWTWAD
                     WSGYTKRKAMEREDQRKLDRINRQRAAIGQKPKAAPPRRR"
     gene            complement(5477..7192)
                     /gene="ilvD"
                     /locus_tag="PK242_05380"
     CDS             complement(5477..7192)
                     /gene="ilvD"
                     /locus_tag="PK242_05380"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOZ93481.1"
                     /translation="MTDSAPLNRRSAHITQGKARAPNRSMYYGMGYTEADFDKPMVGV
                     ANGHSTITPCNSGLQKLADAAVEGIREAGGNAQTFGTPTISDGMAMGTEGMKYSLVSR
                     EVIADCVETCVQGQWMDGCVVIGGCDKNMPGGMMGMLRANVPSIYVYGGTIEPGCLHG
                     CDLNIVSVFEAVGENAAGKISDEELKDIERHAIPGTGSCGGMYTANTMSSAFEALGMS
                     LPYSSTMANPHQEKVDSARESARVLIEAIRRDLKPRDIVTRKAIENAVAVIMAVGGST
                     NAVLHFLAIAHAAGVEWSIDDFERMRKKVPVFCDLKPSGKYLAVDLHKAGGIPQVMKM
                     LLNAGLLHGDCMTITGKTVAETLKDVPDEPPAGQDVIRPITQPLYAQGHLAILKGNLS
                     PEGAVAKITGLKNPVITGPARVFDDEQSALAAILSGKIKAGDVMVLRYLGPKGGPGMP
                     EMLAPTSALIGEGLGESVGLITDGRFSGGTWGMVVGHVAPEAFVGGTIALVHEGDSIT
                     MDAHRLLLQLNVSDEELARRRAGWTPPAPRYTRGVQAKFAFNAASASRGAVLDDFQSP
                     PPMRR"
     gene            7437..9293
                     /gene="ilvD"
                     /locus_tag="PK242_05385"
     CDS             7437..9293
                     /gene="ilvD"
                     /locus_tag="PK242_05385"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOZ93482.1"
                     /translation="MPAYRSKTSTHGRNMAGARALWRATGMKDADFSKPIIAVVNSFT
                     QFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDVIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVIFVSGGPMEAGKVQLLNPQT
                     QTFTARKMDLIDAMVMAADDKASDAEVAEVERSACPTCGSCSGMFTANSMNCLAEALG
                     LALPGNGTVVATHADREQLFRRAGRRIVELARQYYEGGDDRVLPRSVGFKAFENAMTL
                     DIAMGGSTNTILHLLAIAQEAEIAFTMQDIDRLSRVVPQLCKVAPNTDKYHVEDVHRA
                     GGIFAILGELERAGKLHTDVPTVHAPTLGQALAQWDVLRTQDAAVHHFFKAGPGGVPT
                     QVAFSQDARWPSLDLDRAGGCIRSCDHAFSQEGGLAVLRGNIALDGCVVKTAGVDESI
                     LVFEGPAHVVESQDEAVEHILGDQVKAGDVVIVRYEGPKGGPGMQEMLYPTSYIKSKG
                     LGKACALLTDGRFSGGTSGLSIGHCSPEAAAGGAIGLVRNGDRIRIDIPNRSINVLVS
                     DDVLARRRAEQDAVGWKPAQSRPRKVSAALKAYAKLVTSADKGAVRDLSLLD"
     gene            complement(9385..9858)
                     /gene="bfr"
                     /locus_tag="PK242_05390"
     CDS             complement(9385..9858)
                     /gene="bfr"
                     /locus_tag="PK242_05390"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOZ93483.1"
                     /translation="MQGHPEVIDCLKALLRGELTARDQYFLHSRIYEDQGLVRLYERM
                     DHEMQEETQHADAILRRILLLGGQPDMRPRELNPGVDVPDMLRKDLAAEYQVRDNLRQ
                     AIALCDRLHDYVSRDLLLAQLRDTEQDHAYWLEKQIGLIEQIGLQNYLQSQARVD"
     gene            10016..10855
                     /gene="lgt"
                     /locus_tag="PK242_05395"
     CDS             10016..10855
                     /gene="lgt"
                     /locus_tag="PK242_05395"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOZ93484.1"
                     /translation="MLVHPQIDPVALQLGPLAIHWYGLTYLVAFGLFMLLALRRLHHP
                     PYAAITQPAPWTRRDIEDMLFWGVLGVILGGRLGYCLFYKPAYYAANPLEILAVWKGG
                     MSFHGGMLGVIAAMALWARTRGRPWLQVTDLVAPCVPLGLASGRVGNFINGELWGRVA
                     DPSLPWAMVFPGAVLPDGSNPPRHPSQIYQFLLEGVLLFALLWLYARRPRQTGQVSGA
                     FLLGYGVLRFVAEYFRQPDAHLGLLALGMSMGQWLCLPMMAAGLALWVWAGRRPAPLA
                     GTP"
     gene            10922..12322
                     /locus_tag="PK242_05400"
     CDS             10922..12322
                     /locus_tag="PK242_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HOZ93485.1"
                     /translation="MEADRDPAAATGDRPGLSMRERLSAMLERGVDAFGGWNARRFLR
                     ELQQVLDPAVSEVEGGRRAAAVAEEYARATPQQRRACWLLMSEHFSPDVSAIQAAHAA
                     YQAARGSEDESRAEVRLRRAFTAPRTRLLQRFSAYPGGVRFLVDLRAELLPHLARDKR
                     LIALDDELQELFSTWFDVGFLELRRISWDSPASLVEKLIRYEAVHDIRSWADAKNRLD
                     EDRRCYGFFHPRLPDEPLIFVEVALLHDMARSITPLLDEQAPAADLTRAHTAIFYSIS
                     NTQAGLRGVSFGDSLIKRVVETLRTEFAQLKVFATLSPIPGLRRWVDQQAPALLRATP
                     ARLRQALERELGAPLAPDAAALLKALDGVATLDERSAARRWLLAVAARYLGRELADDG
                     RPLDGVARFHLGNGARIEQLNWLADPSPKGLRQSWGLMVNYLYDLRRLDRHRAQLAQG
                     KPPMSHAVQALQTDLL"
     gene            12347..13333
                     /locus_tag="PK242_05405"
     CDS             12347..13333
                     /locus_tag="PK242_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOZ93486.1"
                     /translation="MNTSFTRRQLLLATAAVTALPGGARAQSAWPARPVNLVVPFPAG
                     GGTDAFARPLGAQFTRLTGQNLVIDNKGGGGGTVGAAVAAKAPADGYNFFMGGVHHAI
                     APSVYPKLQYDLLQDFVPLILVARVPQVVVVNPKRVQARTVKDLIELARKNPGKLNYA
                     SAGSGTSHHLAGELFKQQTGTFITHIPYRGAGPALQALIAGEVDLMFDGLGSSAAHIK
                     GGRIQPLMMAGRSRSPAFPDVPSARESGLSDYDVSTWYGIWAPKGTPAEAQAAMIEAV
                     RKSAQTPENKQVWAGQGAEFANLDAAAFGGFVQLEMAKWAKVVKATGGVRID"
     gene            13314..14111
                     /locus_tag="PK242_05410"
     CDS             13314..14111
                     /locus_tag="PK242_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOZ93487.1"
                     /translation="MACGSIELTVDGAIATVVLSHPGKFNAMSRVMWRRLRELFLELP
                     ARADLRCVVVRGEGAQFCAGGDIAEYPAFRFQPDRLRAFHEDEVWGALGALLDCDLPL
                     VAAIAGHCMGAGVEIASCCDIRVAAADARFGAPIARLGFPMAPREAQLVARALGEATA
                     RELLLEAAVLDAAEMQARGFLQRVLPDAAAMHAHALERARRIAALAPQAARLNKQTFR
                     ALNGPSALAGQAPAAIENIVHWAYDYADSAEHREGIDAFLAKRPARF"
     gene            14136..>14574
                     /locus_tag="PK242_05415"
     CDS             14136..>14574
                     /locus_tag="PK242_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ93488.1"
                     /translation="MSQQNLFVALRDAFPADLDAVAVETDNGLCYSWRDLDRASAMVA
                     NLLASLSIPPDASGAPPRVAVQVEKSVEAMILYLATLRAGFVFLPLNTAYQRAEIEYF
                     IGNARPAVVVCTRGNFPWVSQIAFQAGTRHVFTLDDDRSGSLLE"
BASE COUNT         2279 a         4956 c         4922 g         2417 t
ORIGIN      
        1 aggctcgggg cctccaggta cacggggccg ctgccgtcca tgacgaaatg cgcgtggcgc
       61 gtggccgcgc agttggggat catggccacg ggcttgctcg cggcatgcgt ggggtacatc
      121 ttgatcttca cgtccagcac ggtggacagg ccgcccaggc cctgggcgcc aatgcccagc
      181 gcgttgacct tctcgtagag ctccaggcgc agctcctcga cctggtcgag cttctcgccg
      241 cgcgcggcct tggcctgcag ctcgtacatg ttcaggtctt ccatcaggct ttccttggcc
      301 agcagcacgg ccttttccgc cgtgccgccg atgcctatgc ccagcatgcc cggcgggcac
      361 cagcccgcgc ccatggtggg cacggtcttc aagacccagt cgaccaggtt gtcgctcggg
      421 ttcatcatga ccatcttgga cttgttctcg ctgccgccgc ccttggcggc cacggtcacg
      481 tcgagcttgt cgcccggcac gatctgcaca ttgatgacgg cgggggtgtt gtcgcgggtg
      541 ttcttgcgtg cgaacagcgg gtcggccacc acgctggcgc gcagcgtgtt gtccgggtgg
      601 ttgtagccgc ggcgcacgcc ttcgttgacg gcgtcctcca ggctctggtc ggcggcaaag
      661 ccctcaaagc gcacgttcat gccgatcttc aggaacacgt tgacgatgcc cgtgtcctgg
      721 cagatcggac gatggcccgt ggcgctcatc ttgctgttgg tgaggatctg cgccatcgcg
      781 tccttggccg cggggctttg ctcgcgctca taggcgcgcg ccaggtgctg gatgtagtcg
      841 gcggggtggt agtagctgat gtactgcaat gcggcggcga tggattcgat caggtcgcct
      901 tgtttgatga tcgtggtcat gggggcatcc tcggagaagt ctggaatacg catgggcacg
      961 tcggcaattg ccacaggggc attgccgttc tgctaccgtg caccgtatcc gcgtattttg
     1021 acaggccgac ccatgagcga tgctgacaag accacgcgcg tggagcgtga cagctttggc
     1081 ccgatcgagg tgcccgccga gcgcctgtgg ggggcgcaga cgcagcgctc gctgcgccac
     1141 ttcgccatca gcggtgaacg ccagccacgc gagctcattc acgcgctgac ccaggtcaag
     1201 cgggcggcgg cggtggtcaa ccagcgcctg ggtcagctcg atgcgacgct ggcgggcgcc
     1261 atcgtgcgtg ctgccgacga agtgctggct ggccggcatg acgacgaatt cccgctggtg
     1321 gtctggcaga ccggctccgg cacgcagacc aacatgaacc tcaacgaggt gctggccaac
     1381 cgcgccagcg agctgctggg cggcccgcgc ggcgagcagc gcctggtgca tccgaacgac
     1441 cacgtcaacc gcagccagtc cagcaacgac gccttcccga ccgccatgca cgtggctgcg
     1501 gccgaggcga tcgagcggcg gttgctgccc gccctgacgc gcctgcgcgc cacgctggcg
     1561 ggcaagtcgc gcgactttgc cgacatcgtc aagatcggcc gcacccacct gcaggacgcc
     1621 acaccgctca cgctcggtca ggagctgtcg ggctgggtgg cgcagctggc ccatgccggg
     1681 cgccatgtgc gcgcggcgct gccgcatctg cacgaactgg cgctgggcgg cacggccgtc
     1741 ggcaccggtc tgaacgcgcc cgaaggctac gccgaggcgg tggcgcagga gctggcgcgc
     1801 ctgaccgggc tgcccttcgt gaccgcgccc aacaagttcg aggccctggc cagctgcgac
     1861 gccctggtgc aggcgcacgg catgctcaag ggcctggccg ccagcctgac gaagatcgcc
     1921 aacgacgtgc gctggctggc cagcggcccg cgcagcggcc tgggcgagat cagcatcccc
     1981 gagaacgagc ccggctcatc catcatgccg ggcaaggtca acccgaccca gtgcgaggcc
     2041 ctgaccatgc tgtgcgcgca ggtcatgggc aacgacgtgg ccatcagcat cggcggtgcg
     2101 tcgggccatt tcgagctgaa cgtgtttcgc cccatgatcg cccacgcctt tctgcagagc
     2161 gcaaggctgc tggccgacgg catgaacagc ttcaacgacc actgcgcggt gggcatcgag
     2221 cccaaccgcg cgcgcatcga cgagctggtg cagcgctcgc tgatgctggt gacggcgctc
     2281 aatccccaca tcggctacga caaggcggcg caaatcgcca agcgcgcgca ccaggagggc
     2341 ctgagcctgc gcgaggcggc cctggccacc ggctggctga gtgcggcgca gttcgacgcc
     2401 tgggtccggc cgcaggacat ggtgtgatcg aatgctatta caaacatagc tagcggcgct
     2461 tgccacgtat gcgctggaac cgaatttcat tcccaagatc gacgggcgcc ccaagcgccg
     2521 tagccccggc tgcccgggcc cgcccagagt cagtgctttg taagagcctg accggatgat
     2581 gaaaccgtct gtcccgccag cgcgggacgg gtgtgttcaa ctttccccct cttatccagg
     2641 agacaaggca catgagcgcc attcaatcgg ttctggtcga aaaccgcgtg ttccccccca
     2701 gcgaagccat ggtcaaggcg gcccgcatca gcggcatggg cgcctatcag gccctgtacg
     2761 aagaggccga gaaggatttc gaaggtttct gggcgcggcg tgcgcgtgaa cacctggtgt
     2821 gggccaaacc cttcacccag gtgctggatg aatccaacaa gcccttctac aagtggtttg
     2881 ccgacggcca gctcaacgcc agcgccaact gtctggacaa gcacatgggc acgccggtcg
     2941 aaaacaagac cgcgatcatc ttcgaagccg acggcggtga agtcaccaag gtcacctaca
     3001 aggagctgct ggcgcgcgtg agccagttcg ccaacggcct gaaggcccat ggcatccgca
     3061 agggcgaccg ggtgctggtc tacatgccga tgaccatcga gggcctgatc gcgatgcagg
     3121 cctgcgcccg catcggcgcc acgcacagtg tggtgttcgg cggcttctcg gccaaggcgg
     3181 tgcaggaacg catccaggac gccggcgccg tggccgtcat caccgccaac taccagatgc
     3241 gcggtggcaa ggagctgccg ctgaaggcca tcgtcgacga ggcgctgagc ctgggcggct
     3301 gcgagtcgat caagaacgtg tttgtctacg agcgcaccca gaccccctgc aacatggtgg
     3361 ccggccgcga caagaccttc gcccagatgc tgcaagggca gagcaccgag tgcgcgcccg
     3421 agatcgtcga tgccgagcac ccgctgttcg tgctctacac cagcggctcc accggcaagc
     3481 ccaagggcgt gcagcattcc accggcggct acctgctgtg ggcgaagatg accatggagt
     3541 ggaccttcga cctcaaggac agcgacgtct tctggtgcac cgccgacatc ggctgggtca
     3601 ccggccacac ctacatcgtc tacggcccgc tggccgcggg cgcgacgcag atcatgttcg
     3661 agggcgtgcc gacctacccg gacgccggcc gcttctggca gatgatcgag cgccaccagt
     3721 gctcgatctt ctacaccgcg cccacggcca tccgttcgct catcaaggcg tcggaaacca
     3781 acgacaaggt gcatcccagg aactggaacc tgtcttcgtt gcgcatcctg ggcagcgtgg
     3841 gcgagccgat caacccggaa gcctggatgt ggtactaccg caacgtcggc ggcgaacgct
     3901 gcccgatcgt cgacacctgg tggcagaccg agaacggcgg ccacctgatc accccgctgc
     3961 cgggtgccac gccgctggtg ccgggttcct gcactttgcc gctgcccggc gtgtttgccg
     4021 gcatcgtgga cgaggcgggc aacgacatgc cctggggctc gggcggcatc ctggtcatca
     4081 agaagccctg gcccagcatg atccgcacca tctggggcga tccggcgcgc ttcaagtcca
     4141 gctattttcc cgaggagctc aagggttact acctggccgg cgacggagcg gtgcgtgatg
     4201 ccgagcgcgg ctacttccgc gtcaccggcc gcatcgacga cgtgctgaac gtctcgggcc
     4261 accgcatggg caccatggaa atcgaatccg ccctggtggc caagaccgac ctggtggccg
     4321 aagccgccgt ggtcggccgc cccgacgaaa ccaccggcga ggccatctgc gccttcgtgg
     4381 tgctcaagcg tccgctgccg cagggcgacg aagccaagca gatcgccaag gaactgcgcg
     4441 actgggtggc caaggaaatc ggcccgatcg ccaagcccaa ggacatccgc ttcggcgaga
     4501 acctgcccaa gacgcgctcg ggcaagatca tgcgtcgcct gctgcgcagc ctggccaagg
     4561 gcgagcagat cacgcaggac accaccacgc tggaaaaccc ggccatcctg gcgcagctcg
     4621 atcagaccta ctgatcctga gctgctcgac ggcggccccg cgggccgccg ctcggcccca
     4681 ggcacaaaaa aaagcccgcc tgaatcaggc gggctttttg ttgcgggatg tcgcgtgatt
     4741 acttcagcga catgacccag gcggccagct tcttggcctc ggcgtcgttc acgttggcgt
     4801 tgggaggcat gggcaccgcg ccccacacgc ccgagccgcc cttgatgatc ttggcagcca
     4861 gggtatccac ggccttggcg tcactggcgt acttcttggc cacgtccttg taggaggggc
     4921 cgaccagctt cttgtcggtg gcgtggcagg ccatgcagtt cttggccttg gccatggctt
     4981 cgtcggccag ggcaggcgcc gacaccgtca cagcggtcaa agcggcaaaa gtgagcagaa
     5041 cacgcttcat cgtcatatct ccatcgaggg tggttgcgct acagtgagtg aacgcgattg
     5101 taaagtttcc ggttgacgcc gggcaccgat gaaacctgga acacatcaag gaaaaccatg
     5161 tatctgctgg gtttgggaat cattctgttg ctgatgaaat ggcaggaact cgggccggtg
     5221 gctgcctggt cctggtggtg ggtgctgtcg ccgtttgccg gtgcggcgct gtggtggacc
     5281 tgggccgact ggtcgggcta caccaagcgc aaggcgatgg agcgcgagga tcagcgcaag
     5341 ctcgaccgca tcaaccgcca gcgcgcggcg attggtcaga agcccaaggc cgcgccgccg
     5401 cgccgccgct gaacatctat gcccatttgg cctctgccgc ccaccaggaa agcgtcggca
     5461 gttatttgaa tgatagctat cggcgcattg gtggcgggct ctggaaatcg tccagcaccg
     5521 ctccccggct ggcgctcgcc gcattgaagg caaacttggc ctgcacgcca cgcgtgtaac
     5581 gcggcgcggg cggcgtccag ccggcgcgcc ggcgcgccag ttcctcgtcg ctgacgttga
     5641 gctgcagcaa caggcggtgc gcgtccatgg tgatgctgtc gccctcgtgc accagggcga
     5701 tggtgccgcc gacaaaggcc tcgggcgcca catggcccac gaccatgccc caggtgccgc
     5761 cggaaaagcg cccgtcggtg atcagaccca cgctctcgcc cagaccctcg ccgatgagtg
     5821 cactcgtggg tgccaacatc tcgggcatgc cgggcccgcc cttggggccg aggtagcgca
     5881 gcaccatcac gtcgccggcc ttgatctttc ccgacaggat ggcggccagg gccgactgct
     5941 cgtcgtcgaa cacgcgcgcc gggccggtga tgaccgggtt cttgaggccg gtgatcttgg
     6001 ccacggcgcc ctcgggactc aggttgccct tgaggatggc cagatgcccc tgcgcgtaca
     6061 gcggctgggt gatggggcgg atcacgtcct ggcccgcggg tggctcatcg ggcacgtcct
     6121 tgagggtctc ggcaaccgtc ttgccggtga tggtcatgca gtcgccgtgc agcaggccgg
     6181 cgttcagcag catcttcatc acctggggaa tgccgccggc cttgtgcaga tcgacggcca
     6241 ggtatttgcc gctgggcttg aggtcgcaga acacgggcac cttcttgcgc atgcgctcga
     6301 agtcgtcgat ggaccattcg acgcccgccg cgtgggcgat ggccagaaag tgcagcacgg
     6361 cgttggtcga gccgcccacg gccatgatca cggccacggc gttctctatg gccttgcgcg
     6421 tgacgatgtc gcggggtttg aggtcgcggc gtatggcctc tatcagcacc ctggccgact
     6481 cgcgcgcgga atcgaccttc tcctggtgcg gattggccat ggtgctggag tagggcaggc
     6541 tcatgcccag ggcctcgaag gccgaggaca tggtgttggc cgtgtacatg ccgccgcacg
     6601 aacccgtgcc gggaatggcg tggcgttcga tgtccttcag ttcctcgtcg ctgatcttgc
     6661 ccgcggcgtt ctcgcccacg gcctcgaaca cgctcacgat gttcaggtcg cagccatgca
     6721 ggcagccggg ctctatggtg ccgccgtaga catagatgct gggcacgttg gcgcgcagca
     6781 tgcccatcat gccgccgggc atgttcttgt cgcagccgcc tatgaccacg cagccgtcca
     6841 tccactggcc ctgcacgcag gtctcgacac agtcggcgat gacctcgcgg cttaccaggc
     6901 tgtacttcat gccctcggtg cccatggcca tgccgtcgct gatcgtgggc gtgccaaagg
     6961 tctgggcatt gccgcccgcc tcgcgtatgc cctcgacggc cgcatcggcc agcttctgca
     7021 ggccgctgtt gcagggcgtg atggtgctgt ggccgttggc cacgccgacc atgggcttgt
     7081 cgaaatcggc ctcggtgtag cccatgccgt agtacatgga gcggttgggt gcgcgtgcct
     7141 tgccctgggt gatgtgggcc gagcggcggt tcaggggcgc ggaatcggtc atgctgcagt
     7201 cctcgcgcag ttgggggtgg gcgccaagtg tgggcgcctg cgccccgcct gtccagtgcg
     7261 cgcgccggcg cacccggcgc gggtaaggat tgcagcgctg cgacataatc aattggtttg
     7321 cccgcaaacc ggcccaacag gcccaagaag cccaagaggc cctgtgctgc ggtgcggacc
     7381 ggcccccaag cgcacgggcc acagaattcc ccttgcccat ttttcaccta cccacgatgc
     7441 ccgcctaccg ttccaagaca tccacccatg gccgcaacat ggccggtgcc cgcgccctgt
     7501 ggcgcgccac cggcatgaag gacgccgact tctccaagcc catcatcgcg gtggtcaact
     7561 ccttcaccca gttcgtgccc ggccatgtgc acctcaagga cctgggccag ttggtcgcgc
     7621 gcgagatcga ggccgccggc ggcgtggcca aggagttcaa caccatcgcc gtggacgacg
     7681 gcatcgccat gggccatgac ggcatgctgt actcgctgcc cagccgtgac gtgattgccg
     7741 acagcgtgga atacatggtc aacgcgcatt gcgccgacgc catggtctgc atcagcaact
     7801 gcgacaagat cacccccggc atgctcatgg ccgcgatgcg gctcaacatc ccggtgatct
     7861 tcgtttccgg cggacccatg gaagccggca aggtgcagct gctcaacccg cagacccaga
     7921 ccttcaccgc gcgcaagatg gacctgatcg acgccatggt catggccgcc gacgacaagg
     7981 ccagcgacgc cgaggtggcc gaggtcgagc gcagcgcctg cccgacctgc ggctcgtgct
     8041 cgggcatgtt caccgccaac tccatgaact gcctggccga ggcgctgggc ctggcgctgc
     8101 cgggcaacgg cacggtggtg gccacgcacg ccgaccgcga acagctgttc aggcgcgccg
     8161 gccggcgcat cgtggagctg gcgcgccagt actacgaggg cggcgacgac cgcgtgctgc
     8221 cgcgttcggt gggcttcaag gccttcgaga acgccatgac gctcgatatc gccatgggcg
     8281 gctcgaccaa caccatcctg cacctgctgg cgatcgccca ggaggccgag atcgccttca
     8341 ccatgcagga catcgaccgc ctctcgcgcg tcgtgccaca gctgtgcaag gtggcgccca
     8401 acaccgacaa ataccacgtc gaggacgtgc accgtgccgg tggcatcttt gccatcctgg
     8461 gtgagctgga gcgcgccggc aagctgcaca ccgacgtgcc gacggtgcat gcccccaccc
     8521 tggggcaggc gctggcgcag tgggacgtgc tgcgcaccca ggatgcggcg gtgcaccact
     8581 tcttcaaggc cggccccggc ggcgtgccca cgcaggtggc cttcagccag gacgcgcgct
     8641 ggcccagcct ggacctggac cgcgcgggcg gctgcatccg ctcctgcgac cacgccttca
     8701 gccaggaagg cggcctggcg gtgctgcgcg gcaacatcgc cctcgacggc tgcgtggtca
     8761 agaccgcggg cgtcgacgaa tccattctgg tgttcgaggg tccggcccat gtggtcgaga
     8821 gccaggacga ggccgtcgag cacatcctgg gcgaccaggt caaggcgggc gacgtggtca
     8881 tcgtgcgcta cgaaggcccc aagggcggcc ccggcatgca ggaaatgctc tacccgacga
     8941 gctacatcaa gagcaagggc ctgggcaagg cctgcgcgct gttgaccgac gggcgcttct
     9001 cgggcggcac ctcgggcctg tcgataggcc attgctcgcc cgaggccgca gccggcggcg
     9061 ccatcggcct ggtgcgaaac ggcgaccgca tccgcatcga tattcccaac cgcagcatca
     9121 acgtgctggt cagcgacgac gtgctggccc gccgccgcgc cgagcaggac gccgtcggct
     9181 ggaagcccgc ccagtcgcgc ccgcgcaagg tgtcggccgc gctcaaggcc tacgccaaac
     9241 tggtgaccag cgccgacaaa ggggcggtgc gcgacctgtc gctgctcgat tgaactatca
     9301 aaaatatagc ttctggcgct tgcccatcaa gcgctggaag ctgttttgat tcggatcgtg
     9361 gggcgaggcg tcggtggggc gctctcagtc gactcgggcc tggctctgca ggtagttctg
     9421 caacccgatc tgctcgatca ggccgatctg cttttccagc cagtaggcgt ggtcttgctc
     9481 ggtgtcgcgc agctgcgcca gcagcaggtc gcggctgacg taatcgtgca ggcggtcgca
     9541 cagggcgatg gcctggcgca ggttgtcgcg cacctggtac tcggccgcca ggtccttgcg
     9601 cagcatgtcg ggcacgtcga cgccggggtt gagctccctg ggccgcatgt cgggctgccc
     9661 gcccagcagc aggatgcggc gcaggatggc gtcggcgtgc tgggtttcct cctgcatttc
     9721 gtggtccatg cgctcgtaca ggcgcaccag gccctggtct tcatagatgc gggaatgcag
     9781 aaagtactgg tcgcgcgcgg tcagttcgcc gcgcagcagc gccttgaggc aatcgatgac
     9841 ttcgggatgg ccttgcatgg gggttctctt cgggaaggtc agggccgtca gtatcgcgtc
     9901 atcggcgtgg tcagaccctg tgctggcgca aaccgtgttc acggcaccac gcgtcggtgc
     9961 gcagcggtgg cgccaccggg gaggatgccg gctggcctgc ggcacaatcg ccgccatgct
    10021 ggtgcacccc cagatcgatc ccgtcgcgtt gcagctcggc ccgctggcca tccactggta
    10081 tgggctgacc tatctggtgg cgttcggcct gttcatgctg ctggcgctgc ggcgcctgca
    10141 ccacccgccc tacgcagcca tcacgcaacc ggcgccgtgg acgcggcgcg acatcgagga
    10201 catgctgttc tggggcgtgc tgggcgtgat cctgggcggt cggctgggtt actgcctgtt
    10261 ctacaagcca gcgtactacg cggccaaccc gctggagatc ctggcggtgt ggaagggcgg
    10321 catgagtttt cacggcggca tgctgggcgt gatcgccgcc atggcgctgt gggcgcgcac
    10381 gcgcgggcgg ccctggctgc aggtgaccga tctggtggcg ccctgcgtgc cgctgggcct
    10441 ggcttcggga cgcgtcggca acttcatcaa cggtgagctg tgggggcggg tggccgaccc
    10501 ctcgctgccc tgggccatgg tgtttcccgg cgcggtgctg cccgacggca gcaatccgcc
    10561 gcgccacccg tcgcagatct accagttcct gctggagggt gtgctgctgt tcgcgctgct
    10621 gtggctgtac gcgcgccggc cgcgccagac cggccaggtc agcggcgcct ttctgctggg
    10681 ctatggggtg ctgcgcttcg ttgccgagta cttccgacag cccgacgccc acctggggct
    10741 gctggccctg ggcatgagca tgggccagtg gctgtgcctg cccatgatgg ccgccggcct
    10801 ggccctgtgg gtctgggccg ggcggcgacc ggcgccgctg gcgggcacgc cttgacggct
    10861 tgcggaccat gagcgatttt cggcttcggc gcttatccat caagcgccaa cagctatgaa
    10921 tatggaagca gatcgcgacc ctgcagcggc caccggagac cgtccggggc tcagcatgcg
    10981 cgagcgcctg agcgccatgc tggaacgtgg cgtcgatgcc ttcggcggct ggaacgcgcg
    11041 ccgctttctg cgcgagctgc agcaggtgct cgacccggcg gtcagcgagg tcgagggcgg
    11101 ccgccgcgcc gccgcggtgg ccgaggaata cgcccgcgcc acgccgcagc agcggcgcgc
    11161 atgctggctg ctgatgagcg agcacttcag ccccgacgtg tcggcgatcc aggccgcaca
    11221 cgccgcctac caggccgcgc gcggcagcga agacgagtcg cgcgccgagg tgcggctgcg
    11281 ccgcgccttc accgcgccgc gcacgcgcct gctgcagcgc ttttccgcct atccgggcgg
    11341 tgtgcgcttt ctggtcgacc tgcgcgccga gttgttgccc catctggccc gggacaagcg
    11401 cctgatcgcg ctcgacgacg aactgcagga gctgttttcc acctggttcg acgtcggctt
    11461 tctggagctg cggcgcatca gctgggattc gcccgcctcg ctggtcgaaa agctgatccg
    11521 ctacgaggcg gtgcacgaca tccgcagctg ggccgacgcc aagaaccggc tcgacgaaga
    11581 ccggcgctgc tacggcttct ttcacccgcg cctgcctgac gagccgctga tcttcgtcga
    11641 ggtggcgctg ctgcacgaca tggcccgcag catcaccccg ctgctggacg agcaggcgcc
    11701 ggccgccgat ctgacgcgtg cccacacggc catcttctac tccatcagca acacccaggc
    11761 tggcctgcgc ggggtgagtt ttggcgactc gctgatcaag cgcgtggtgg aaaccctgcg
    11821 caccgagttt gcgcagctga aagtgtttgc caccctgtcg cccatccccg gcctgcgccg
    11881 ctgggtcgac caacaggcgc cggcgctgct gcgcgccacg ccggcgcgcc tgcggcaggc
    11941 gctggagcgc gagctgggcg cgccgctggc gcccgacgcc gcggccctgc tgaaggcgct
    12001 ggacggtgtg gcgacgctgg acgagcgctc ggccgcgcgc cgctggctgc tggcggtggc
    12061 ggcgcgctac ctggggcgcg agctggccga cgacggccgc ccgctggacg gcgtggcgcg
    12121 ctttcacctg ggcaacggcg cgcgcatcga gcagctcaac tggctggccg acccttcgcc
    12181 caagggcctg cgccagtcct ggggcctgat ggtcaactac ctgtacgacc tgcggcgtct
    12241 ggatcggcat cgggcccagc tggcacaggg aaaacccccg atgtcgcacg ccgtgcaggc
    12301 attgcaaact gatctgcttt gacttcaaac gaaagaggag atgcgcatga acacgtcgtt
    12361 cacccgtcgg caactgctgc tggccaccgc cgccgtcacc gccctgccgg gcggcgcccg
    12421 cgcgcaatcg gcctggccgg cgcggcccgt caacctggtg gtgccctttc cggccggtgg
    12481 cggcaccgac gcctttgcgc gcccgctggg cgcccagttc acgcgcctga ccgggcagaa
    12541 cctggtgatc gacaacaagg gcggtggcgg cggcacggtg ggcgccgcgg tggcggccaa
    12601 ggcgccggcc gacggctaca acttcttcat gggcggcgtg caccacgcca ttgcgcccag
    12661 cgtgtacccc aagctccagt acgacctgct gcaggacttc gtgcctctga ttctggtggc
    12721 gcgcgtgccg caggtggtgg tggtcaaccc caagcgggtg caggcgcgca ccgtcaagga
    12781 cctgatcgag ctggccagga agaacccggg caagctcaac tacgcgtcgg ccggcagcgg
    12841 cacctcgcac catctggccg gcgagctgtt caaacagcag accggcacct tcatcaccca
    12901 cattccctac cgcggcgccg gcccggcgct gcaggccctg atcgcaggcg aggtggatct
    12961 gatgttcgac gggctgggct cgtcggcggc gcacatcaag ggtggccgca tccagccgct
    13021 gatgatggcc gggcgcagcc gaagccccgc ctttccggac gtgccctcgg cgcgtgagtc
    13081 ggggctgtcc gactacgacg tcagcacctg gtacggcatc tgggccccca agggcacgcc
    13141 cgccgaggcc caggcggcca tgatcgaggc cgtgcgcaag tccgcccaga cccccgaaaa
    13201 caagcaggtc tgggccggtc agggggcgga attcgccaac ctcgacgccg ccgccttcgg
    13261 cggtttcgtg cagctcgaaa tggccaagtg ggccaaggtg gtgaaggcga ccggtggcgt
    13321 gcggatcgat tgagttgacc gtcgacggcg ccatcgcgac cgtcgtgctg tcgcatcccg
    13381 gcaagttcaa cgccatgtcg cgcgtcatgt ggcgccggct gcgcgagctg ttcctggagc
    13441 tgcccgcgcg ggccgacctg cgctgcgtgg tggtgcgtgg tgagggtgcg cagttctgcg
    13501 ccggcggcga cattgccgaa tacccggcgt tccgctttca gcccgaccgt ctgcgcgcct
    13561 tccacgagga cgaggtgtgg ggcgcgctgg gcgcgctgct ggactgcgac ctgccgctgg
    13621 tggccgccat tgccggccac tgcatgggcg cgggggtgga aatcgccagc tgctgcgaca
    13681 ttcgcgtcgc cgccgccgat gcgcgctttg gcgccccgat cgcccgcctg ggctttccga
    13741 tggcaccgcg cgaagcgcag ctggtggcgc gggcgctggg cgaggccacg gcgcgcgagc
    13801 tgctgctgga ggcggcggtg ctcgatgccg cagaaatgca ggcgcgcggc tttctgcagc
    13861 gcgtgctgcc cgacgccgcg gccatgcacg cccatgcgct cgagcgtgcc cgccgcatcg
    13921 ccgccctggc gccgcaggcg gcgcgcctga acaagcagac ctttcgtgca ctgaatgggc
    13981 cttcggcgct tgctgggcaa gcgccggcag ctatcgaaaa catagtgcat tgggcctacg
    14041 actacgccga cagcgccgag caccgcgagg gcatcgacgc ctttctggca aagcgcccgg
    14101 cgcgtttctg agcttccctc ctacctctga accccatgtc ccagcaaaac ctgttcgttg
    14161 ccctgcgcga cgcctttccc gccgatctgg acgccgtggc cgtcgaaacc gacaacggcc
    14221 tgtgctattc gtggcgcgac ctcgatcgcg ccagcgccat ggtcgccaac ctgctggcct
    14281 cgctgtccat cccgcccgat gccagcggcg cgccgccgcg cgtggcggtg caggtggaaa
    14341 agtcggtcga ggccatgatc ctgtacctgg ccacgctgcg cgccggcttc gtgttcctgc
    14401 cgctcaacac cgcctaccag cgcgccgaga tcgagtactt catcggcaac gccaggccag
    14461 ccgtggtggt gtgcacccgc ggcaacttcc cctgggtcag ccagatcgcc tttcaggccg
    14521 gcacgcgcca cgtgttcacg ctggacgacg accgcagcgg cagcctgctg gagc
//
LOCUS       DAOOOG010000047        14498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4483_length_14498_cov_30.917953, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000047 DAOOOG010000000
VERSION     DAOOOG010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14498
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4483_length_14498_cov_30.917953"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PK242_05420"
     CDS             <1..1125
                     /locus_tag="PK242_05420"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOZ93489.1"
                     /translation="VLAHELSHITQRHIPRLLEQEKRQTPLMLAAMVAGMIAASRNPQ
                     AGAALAVGGQAAVIQQQLNFSRDMEREADRIGYGVLVQAGFDGQGFVGMFEKLQNATR
                     INDNGDWPYLRSHPLTTQRIADMQQRAPAAARGAAPELEALLVAARARVLGRPGVDVL
                     RAWAGEPDQPGFAARPLAARAGALYAAALARAQLRELPRAQALASQLAQAVAGDAAAA
                     RQARLLQAELALAADQPARALELLPPERPSGRGQAGRATLLLRAQAQTASGQAAQAAD
                     ALQVWVSDQPQDAGAWQALAQAHAAQGQTLRALRAEGEVPMAQLDYAGAVDRWRAAQD
                     AARRAAGAADLIEASIVDTRLRQAQALLQQQRQEEQKARR"
     gene            complement(1303..1647)
                     /locus_tag="PK242_05425"
     CDS             complement(1303..1647)
                     /locus_tag="PK242_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOZ93490.1"
                     /translation="MARMILKWLLSAAALLGVAYIYGGVSVSSFSTALLAALVIGLLN
                     MLVRPVLVLLTIPITLLTLGLFLFVINALMFWAASGLVGGFNVSGFWAALLGSLIYSA
                     LGLVIDALLERA"
     gene            complement(1712..2404)
                     /locus_tag="PK242_05430"
     CDS             complement(1712..2404)
                     /locus_tag="PK242_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOZ93491.1"
                     /translation="MEFFSAPWWSALLAIVLIDLVLAGDNAIVIALAARNLPRERQRQ
                     AIVWGTVGAIGVRTLMTLVVVWLLKIPGLMALGGLGLLWIAYKLVADTGADESEHGPA
                     ASTFWGAMKTIVIADALMGIDNVLGVAGAAHGAMDLVVIGLLISVPIVVFGSTVVLRL
                     VERFPVIIQLGAAVLAWTAASMIAGEPLLAGWFGPREAPAQALRWALAALCIVGVLGA
                     AWLMRTRLQNDP"
     gene            2800..3474
                     /locus_tag="PK242_05435"
     CDS             2800..3474
                     /locus_tag="PK242_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOZ93492.1"
                     /translation="MRSVQGWQGLRGWAARRARAAGRPSVPPRAHSALEDVQAILTGC
                     LFVALALVFMRDSRLLPGGVTGLAFIVHYASGWALGAALFVLNLPFYLFAWRALGWRF
                     TLKTFVAVAVLALYTELLPRLVGLARLDPVFSAVMSGLLAGTGILILMRHQASLGGLG
                     ALAFYLQQRRGWRAGTVQMLCDALILGSALWLLPPGRVALSVLSAAALNFVIAINHRP
                     DRYHGI"
     gene            complement(3657..5351)
                     /locus_tag="PK242_05440"
     CDS             complement(3657..5351)
                     /locus_tag="PK242_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOZ93493.1"
                     /translation="MDELTFDHIIVGAGTAGSLLANRLSQDAGKRVLLIEAGGKDDYH
                     WIHIPVGYLYCIGNPRTDWLYQTEPADGLNGRTLRYPRGKVLGGCSSINGMLYLRGQA
                     RDYDHWAALTGEDEWRWEHCLPDFMAHEDHYRLDSDSRSSGAVGGANDQDWSGFHGHG
                     GEWRVERQRLRWQVLDAFAEAAVQAGLPASEDFNRGNNEGVGYFHVNQKNGWRWNTAK
                     AFLRPTCYGRSNFELWTRGQVMRLALERDADGALRCAGAEVWTGGEMQKARLAPGGEL
                     LLAAGSIGSPQILQLSGIGPAALLQQHGIAVVADLPGVGANLQDHLQIRSVYRVQGAQ
                     TLNQLASTLWGKARIGLQYLWSRSGPMSMAPSQLGAFTRSSPAYAWPNLEYHVQPLSL
                     DAFGEPLHRFPAITASVCNLNPTSRGTVRIRSHRFEDAPAIAPNYLSTDEDRQVAADS
                     LRVTRRIMAQPAMARFAPEEYKPGVQYQSDEELARLAGDIASTIFHPVGTTKMGRDDD
                     PMAVLDPRLRVRDGAGGLIRALRVVDAGAMPTITSGNTNSPTLMMAEKAARWIRGA"
     gene            complement(5529..6236)
                     /locus_tag="PK242_05445"
     CDS             complement(5529..6236)
                     /locus_tag="PK242_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93494.1"
                     /translation="MLAAALALPLGGCAGSAPTTRGVVRGQDFQPAELLQSEGNRVAH
                     NAMQANQRSLLLLADKLYRRNPAEWRKRASTREAALADIERSLLTGQPWPPLGDRRDV
                     AALSYALAPDFMGDRVAAFVIACADTIVTAHGGKREFYYLDGVDPQHLYNAARNMEVA
                     LWILNSRRDAQGQPLLLANDIGPDRRNLSFEREFGKIIGRLDLLASYATERYRRAVIG
                     YVQGVVAAPLLAFLPVR"
     gene            complement(6310..6894)
                     /locus_tag="PK242_05450"
     CDS             complement(6310..6894)
                     /locus_tag="PK242_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOZ93495.1"
                     /translation="MALQGPFFGKRDEPTTPRGPGSTPLSGTSALGSAGAAGAQPFAA
                     PSAAASPAAPASTPVAASVTESGAKLIVGPNIKLKGVEITDCDTLVVEGMVEATMDSR
                     VIQIAEQGSFKGSAEIDIAEVRGSFDGNLTVRQKLVIFATGKVTGKIRYGKIVIEEGG
                     QLSGEISFGTDKDHKSDKPAASAGSSKGEQLAVA"
     gene            7079..8200
                     /locus_tag="PK242_05455"
     CDS             7079..8200
                     /locus_tag="PK242_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ93496.1"
                     /translation="MSAEPPRIDHAPADAAARVLLRGEWTAAWLSTRPVWRATQAALG
                     GVAAGGSWDLRQLAALDHLGALVLWHHWGRRWPAELLSSDEQRALLERVAAGAAPAPP
                     AEARAGWSEGVEALGLRVTDAAFQVRDFVTLMGQLLLDALRLLARPGRGPWRDFSGHL
                     YRIGAQALPITALVGFLIGVVLAYLMSQVLRQFGAEAFIVNILGLALIRELGPILAAV
                     LLAGRSGSAITAQIGVMRVTEELDAMRVLGIPAGFRLVLPRALALAIAMPLISVWTTL
                     AALVGGMLASDITLGITPAYFVEALPKAVRIPNLTLAVSKSVVFGVSIALIGCHYGLR
                     VKPNTESLGQGTTASVVASITAVILIDALFAVLFKSVGI"
     gene            8367..9221
                     /locus_tag="PK242_05460"
     CDS             8367..9221
                     /locus_tag="PK242_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ93497.1"
                     /translation="MEAAVQASDAVDTALAPGQPVIEVRGLWSVFALPGGGQHVVHQD
                     LDLTVRRGEVLTLVGGSGTGKTVLLRHMLGLTRPARGQATVLGRPAGELAREGAAARV
                     GMLFQQGALFSAFSVLDNIAFALRESGALPPALVREAALLKLQMVGLKASDAHKMPAE
                     LSGGMIKRVALARALIMDPPLLMLDEPTAGLDPDSSDAFVALLRALHQELGLTVVMVT
                     HDLDTLFELSSRVAVLADKRVIINAPVPEVLAFEHPFIRHFFLGERGRRAMELLRQRQ
                     HARPAQGD"
     gene            9226..10215
                     /locus_tag="PK242_05465"
     CDS             9226..10215
                     /locus_tag="PK242_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOZ93498.1"
                     /translation="MENKAHALAAGTFVVLVTALLIGLAMWLLRDVTNTRAYEIVSHN
                     AVSGLQPQAAVRFKGVAVGKVTRISFDPAERGRVLVTLAIAPDAPITRATVAELAYQG
                     VTGLSFVQLDDDGSDAAPLPPGPNGVPRIPLKPGLLGQLGDRAQDLIGKLDTSAQRIN
                     QLLAPDNQAVLTGALGDIGAAARSVDRLARTADQTLQAQLATSRTSIPRLITQATDTL
                     RTLQDATTEARTVIHTLGQAAQQTQQGVARVTAPGGVIDRLDQGVQTINASTLPRLHG
                     LADAADQTLRRVDRVARTLGDNPQALVYGSGPVPPGPGEAGFVPPPAHPTSPR"
     gene            10290..10877
                     /locus_tag="PK242_05470"
     CDS             10290..10877
                     /locus_tag="PK242_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOZ93499.1"
                     /translation="MAALALAGCALPLPDKPPRTEPYDLGPPPAAGAAATPGAPLALA
                     PIEASPALDSQAMVYRLLYAGAGQQPRPYAQARWSMPPPQLLALRLRQRLAATRPVLD
                     AGSALAALQLRVELDDFAHEFSSPQTSEGVVRLRVTALAAGARAQPLLGQRSFSVRRP
                     APTPDAAGGAQALRDATDEALRQLIDWLAGLPASG"
     gene            11008..11697
                     /locus_tag="PK242_05475"
     CDS             11008..11697
                     /locus_tag="PK242_05475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOZ93500.1"
                     /translation="MGQTIELKAADGQLVPAYLAPPAGPARGAVVVLQEIFGVNRHIR
                     AVADGFAGDGYLALAPALFQRLRARVELGYEEADVKAGLALKAAAEALPAPGVLADVQ
                     AAIDHAAQASGGKVGVIGYCWGGLLSWRAAALLDRLAAAVPYYGGGMTQPAELARQPR
                     VPVLAHLSDHDASVPLDGVAALRRAHPEVELHLYPAAHGFNCDQRAAWQPAAAALARE
                     RTLAFFARHLR"
     gene            complement(11717..12652)
                     /locus_tag="PK242_05480"
     CDS             complement(11717..12652)
                     /locus_tag="PK242_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ93501.1"
                     /translation="MDSLDLIQTFREVARRGSFSGAARALGMSPANVSKYIAQLEARF
                     GLRLFHRTTRKVTLTEAGELLFERSGALLELIDFTEGEMHERATRPVGRLALTVPHAL
                     MASELPALLGRFMQRYPEVTLHLHVTNRLVNLVDEGVDLAFRIGPILQPNLIVRRLLR
                     LDHVAAATPDYWDRRGRPAHPSELLEHDQLAFALPGEAPVWQFQDRGKPLELRLTPRF
                     TATDPAPLRTWALQGLGVVWGTVHWLADALASGTLEPLLHDYSPPEIWLYVAYMQRRH
                     SSAALRALLDYLDAQFQPPPTPTLPAPGRRRVDSR"
     gene            complement(12676..13800)
                     /locus_tag="PK242_05485"
     CDS             complement(12676..13800)
                     /locus_tag="PK242_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOZ93502.1"
                     /translation="MHIVEFTGGSLRFADAVPAAAPADGFVWIYLDREDFARHLPELQ
                     EAAHRLGGSPLLDVHAKDLANPAHPSGYDATSVYDLVVFRRLASLAEVQLNGNHDPHT
                     GAAGAPRAPKPQPFERIDSRAVGFVVFDRLLISVHPPGCQTAVGFLQRQADDARLSVD
                     SASARNRVPQGPDDLVLRMLNAMVDSYLDLRRDLSEALEQAQVELLKPHPAPHTWGAL
                     MHARGRLHGLQDLCEEQQDAMQEWLDTLRELPLSTYGPDPLLAQQRHDQLVARARDVM
                     EHIERVLRHARRLEQAAETAVQIHFAAQGQRTNDTMRALTAITAVFLPLNLFTGFFGM
                     NFEHLPLIHSVEGMWLALLLLVVLALGIVVLFWRRRYLTR"
     gene            complement(13863..>14498)
                     /gene="hemB"
                     /locus_tag="PK242_05490"
     CDS             complement(13863..>14498)
                     /gene="hemB"
                     /locus_tag="PK242_05490"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOZ93503.1"
                     /translation="DVALDPYTSHGQDGWLDKTGYILNDETVGQLVKQALTQAAAGVD
                     IVAPSDMMDGRIGAIRDALEKKGHIHTRIMAYSAKYASAFYGPFRDAVGSAKNLGASD
                     KKVYQMDPGNSDEALREVAIDIAEGADMVMVKPGLPYLDIVRRVKDQFGVPTFAYQVS
                     GEYAMLKAAAQNGWLDHDRVMMESLLAFKRAGADGVLTYFARDAARLLQNQ"
BASE COUNT         1985 a         5139 c         5304 g         2070 t
ORIGIN      
        1 gtgctggcgc acgagctgtc gcacatcacg cagcgccaca tcccgcgcct gctggagcag
       61 gaaaagcgcc agacgccgct gatgctggcg gccatggtgg ccggcatgat cgccgccagc
      121 cggaacccgc aggccggcgc cgcgctggcg gtgggcgggc aggcggcggt gatccagcag
      181 cagctcaact tctcgcgcga catggagcgc gaggccgacc gcatcggcta cggcgtgctg
      241 gtgcaggccg gcttcgacgg ccagggcttc gtcggcatgt tcgaaaaact gcagaacgcc
      301 acccgcatca acgacaacgg cgactggccc tacctgcgca gccacccgct gaccacccag
      361 cgcatcgccg acatgcagca gcgtgccccg gcggccgcgc gcggcgccgc gcccgagctg
      421 gaggcgctgc tggtggcggc gcgtgcacgc gtgctcggcc gccccggcgt cgacgtgctg
      481 cgcgcctggg ccggcgagcc cgaccagccc ggttttgccg cccgcccgct ggccgcgcgc
      541 gccggcgcgc tgtacgccgc cgcgctggcg cgggcgcagc tgcgcgagct gccgcgcgcg
      601 caggcgctgg ccagccagtt ggcgcaggcg gtggccggcg acgccgcggc ggcgcgccag
      661 gcgcgcctgc tgcaggccga actggcgctg gcggccgacc agccggcgcg cgcgctcgaa
      721 ctgctgccgc ccgagcgtcc gagcggccgc ggccaggccg gccgcgccac gctgctgctg
      781 cgcgcccagg cccagaccgc cagcggccag gcagcgcagg cggccgacgc gctgcaggtc
      841 tgggtcagcg accagccgca ggacgccggc gcctggcagg ccctggccca ggcccatgcg
      901 gcccaggggc agacgctgcg cgccttgcgc gccgagggcg aggtaccgat ggcccagctg
      961 gactacgccg gcgccgtgga ccgctggcgc gccgcgcagg acgcggcgcg ccgcgccgcg
     1021 ggcgccgccg acctgatcga agccagcatc gtcgacaccc ggctgcgcca ggcgcaggcg
     1081 ctgctgcagc agcagcggca ggaagagcag aaggcgcgcc gctgaagcgc agcgagcgcg
     1141 gtgcgggcgg gtgcgtagcg tgctcaccca agaggtgaag gccatcgcag ccgccagatc
     1201 gcgcagcggt gcggtctgcc cgaagttgac aagcggtcaa caaggctcca ccgcttgcct
     1261 ggcaagcgca ggcagctatc taaacaatag caaatcgagc gttcaggcgc gctccagcag
     1321 ggcgtcgatc accagcccca gcgccgaata gatgagcgag cccagcagcg cggcccagaa
     1381 gccgctgacg ttgaagccgc ccaccaggcc cgaggcggcc cagaacatca gggcgttgat
     1441 gacgaacagg aacagcccca gcgtcagcag cgtgatcggg atggtcagca gcaccagcac
     1501 cggccgcacc agcatgttca gcaggccgat caccagcgcc gccagcagcg cggtggaaaa
     1561 gctgctcacg ctcacgccgc catagatgta ggccacgccc agcagcgcgg cggcgctgag
     1621 caaccatttg aggatcatcc ttgccatggg acgcagcata gcgcagcgcg gccgcaccta
     1681 cggcttcgcg ttccagacca tggcccatgg gtcatgggtc attctgaagg cgagtgcgca
     1741 tcagccaggc cgcgcccagc acgccgacga tgcacagcgc cgccagcgcc cagcgcagcg
     1801 cctgcgccgg cgcctcgcgc gggccgaacc agccggccag cagcggctcg ccggcgatca
     1861 tcgacgccgc cgtccacgcc agcaccgccg cgcccagctg gatgatgacc ggaaagcgct
     1921 ccaccagccg cagcaccacg gtggagccga acaccacgat cggcacgctg atcagcaggc
     1981 cgatcaccac caggtccatg gcgccgtgcg ccgcgccggc cacgcccagc acgttgtcga
     2041 tgcccatcag cgcatcggcg atcacgatgg tcttcatggc gccccagaag gtgctggccg
     2101 ccgggccgtg ctcgctctcg tccgcgccgg tatcggccac cagcttgtag gcgatccaca
     2161 gcaggcccag cccgcccagc gccatcaggc ccgggatctt cagcagccac accaccacca
     2221 gcgtcatcag cgtgcgcacg ccgatggcgc ccaccgtgcc ccagacgatg gcctgccgct
     2281 ggcgctcgcg cggcaggttg cgcgccgcca gcgcgatgac gatggcgttg tcgccggcca
     2341 gcacgaggtc gatcaggacg atggccagca aggcggacca ccagggggca gagaaaaatt
     2401 ccatggttgc agctccagac agggacaagg cgcgacgccg aagcggcacc tgccggggtg
     2461 gccgccgcac gggcactgtc ttgggggcaa ggcagcaaga ccgcagcttc gaccgacccc
     2521 ggcgcgtgcc gggcaggatc gaaggtcttg ctcggtggcc gacgcggtct gccacccgcc
     2581 gggccggaag ccgcaaggct tcgtattgac gacccggcga aaccgcccgt ggccggtgcg
     2641 caaggcacca ggcgggccgg gcggcccagg gagctactcc ccttcgcgaa agtctgcgat
     2701 tacaccatgg gtgccaacac gccgcaagcg gccgccggcg gcagccaagc ttggcctggc
     2761 gcaagggcgg ccggcgctgg cggccctaac atcgccgcca tgcgcagcgt tcaagggtgg
     2821 caggggcttc ggggctgggc ggcgcggcgc gcgcgggccg ccggccggcc gtcggtgccg
     2881 ccgcgcgccc acagcgcgct ggaagacgtg caggccatcc tgaccggctg cctgttcgtc
     2941 gccctggcgc tggtgttcat gcgcgacagc cggctgctgc cgggtggggt caccgggctg
     3001 gccttcatcg tgcactacgc cagcgggtgg gcgctgggcg cggcgctgtt cgtgctcaac
     3061 ctgcccttct acctgttcgc ctggcgcgcg ctgggctggc gcttcacgct caaaaccttc
     3121 gtggcggtgg cggtgctggc gctctacacc gagctgctgc cgcggctggt cgggctggcg
     3181 cggctcgacc cggtgttctc ggccgtcatg agcggcctgc tggccggcac cggcatcctg
     3241 atcctgatgc gccaccaggc cagcctgggc ggcctgggcg cgctggcctt ctacctgcag
     3301 cagcgccgcg gctggcgcgc cggcacggtg cagatgctgt gcgacgcgct gatcctgggc
     3361 tcggccctgt ggctgctgcc gccggggcgg gtggcgctgt cggtgctgtc ggcggcggcg
     3421 ctcaacttcg tcatcgccat caaccaccga ccggaccgct accacggcat ctgaacctgg
     3481 tgctgaatcg cgttccaaac catgtcccat ggggcatcga agcacagcgc ggtcgtccgt
     3541 gtccgggcca gcggcaaggc ctggatcgct ataaaaacaa gagctgatgg cgcgccatgg
     3601 gcgggcgcca gggccccaaa catgccccaa tcgaccgcgg tggggccttg cgccccttac
     3661 gcgccgcgga tccagcgcgc cgccttttcg gccatcatca gcgttgggct gttggtgttg
     3721 ccgctggtga tggtgggcat ggcgccggcg tcgaccacgc gcagcgcgcg gatcaggccg
     3781 ccggcaccgt cgcgcacgcg cagccggggg tcgagcaccg ccatcgggtc gtcgtcgcgc
     3841 cccatcttgg tggtgcccac cgggtgaaag atggtgctgg cgatgtcgcc ggccagacgc
     3901 gccagctctt cgtcgctctg gtactgcacg ccgggcttgt actcttcggg cgcaaagcgc
     3961 gccatggccg gctgggccat gatgcggcgc gtcacgcgca ggctgtcggc cgccacctgg
     4021 cggtcttcgt cggtgctcag gtagttgggg gcgatggccg gcgcatcctc gaagcggtgg
     4081 ctgcggatcc gcaccgtgcc gcggctggtg gggttgaggt tgcacacgct ggcggtgatg
     4141 gccgggaagc ggtgcagcgg ctcgccgaag gcgtccaggc tgagcggctg cacgtggtat
     4201 tcgaggttgg gccatgcgta ggccggcgac gagcgcgtga aggcccccag ctggctgggc
     4261 gccatgctca tcgggccgct gcgcgaccac aggtactgca ggccgatgcg cgccttgccc
     4321 cagagcgtgc tggccagctg gttcagcgtc tgcgcgccct gcacccgata gacgctgcgg
     4381 atctgcaggt ggtcctgcag gttggcgccc acgccgggca ggtcggccac cactgcaatg
     4441 ccgtgctgct gcagcagcgc ggccgggccg atgcccgaca gctgcaggat ctgcggcgag
     4501 ccgatgctgc cggcggccag cagcagctcg ccgccgggcg ccaggcgggc cttctgcatt
     4561 tcgccaccgg tccagacttc ggcgccggcg cagcgcagcg caccgtcggc gtcgcgctcc
     4621 agcgccagcc gcatcacctg gccgcgcgtc cacagctcga aattgctgcg accgtagcag
     4681 gtcgggcgca gaaacgcctt ggcggtgttc cagcgccagc cgttcttctg gttgacgtga
     4741 aagtagccca cgccctcgtt gttgccgcgg ttgaagtcct cgctggccgg aaggccggcc
     4801 tgcaccgccg cctcggcaaa ggcgtccagc acctgccagc gcagccgctg gcgctccacc
     4861 cgccattccc cgccgtgccc gtgaaagccc gaccaatcct ggtcattggc gccaccgacc
     4921 gcaccggaac tgcgcgaatc gctatcaaga cgatagtgat cctcgtgtgc catgaagtcc
     4981 ggcaggcaat gctcccagcg ccactcgtcc tcgcccgtca gcgccgccca gtggtcgtag
     5041 tcgcgcgcct ggccgcgcag gtacagcatg ccgttgatgc tgctgcagcc gcccagcacc
     5101 ttgccgcgcg ggtagcgcag cgtgcgcccg ttcaggccgt cggccggctc ggtctggtag
     5161 agccagtcgg tgcgcgggtt gccgatgcag tacaggtagc cgaccggaat atggatccag
     5221 tggtagtcgt ccttgccgcc ggcctcgatc agcagcacac gcttgccggc gtcctgcgac
     5281 agccggttgg ccagcaggct gccggccgtg ccggcgccca cgatgatgtg gtcgaaagtc
     5341 agttcgtcca tgccatgtct cctgcgctgg cagcatgcca caagcccggc gcggcaggaa
     5401 cccatggggg gcacgggcgt gtcggtcacc ccacgcgggc ggcggcaggc cgatgccccc
     5461 gagcgtgccg tcagaacggc aaccttccca tgacgaagga caaaggagcg cgcggcgccc
     5521 gggtggcgtc accggaccgg cagaaaggcc agcagcggcg ccgccaccac gccctgcacg
     5581 tagccgatca cggcgcggcg gtagcgttcg gtggcgtagc tggccagcag gtccaggcgg
     5641 ccgatgatct tgccgaactc gcgctcgaag ctcaggttgc ggcggtccgg gccgatgtcg
     5701 ttggccagca gcagcggctg gccctgggcg tcgcgccggc tgttgaggat ccacagcgcc
     5761 acttccatgt tgcgtgcggc gttgtagaga tgctgcggat cgacgccgtc gaggtagtag
     5821 aactcgcgct tgccgccgtg cgcggtgacg atggtgtcgg cgcaggcgat gacgaaggcg
     5881 gccacgcggt cgcccatgaa gtcgggcgcc agcgcgtacg acagcgccgc cacgtcgcgc
     5941 cggtcgccca gcgggggcca gggctggccg gtgagcaggc tgcgttcgat gtcggccagc
     6001 gccgcctcgc gcgtgctggc gcgcttgcgc cattcggccg ggttgcggcg gtacagcttg
     6061 tcggccagca gcagcaggct gcgctggttg gcctgcatgg cgttgtgtgc cacgcggttg
     6121 ccttcgctct gcagcagttc ggcgggctga aagtcctgcc cgcgcaccac gccgcgcgtg
     6181 gtgggggcgc tgccggcgca gccgcccagc ggcaaggcca gggccgcggc cagcaaaaag
     6241 gccgtcgcgc ggacggcctg cggggcgggc aggccagcgg cggcgccgcc ggcccgatgg
     6301 cgccaacgct caggcgacgg cgagctgctc gcccttgctg ctgcccgcgc tggcggcggg
     6361 cttgtcgctc ttgtgatcct tgtcggtgcc aaagctgatc tcgccggaca gttgtccgcc
     6421 ttcctcgatc acgatcttgc catagcggat cttgccggtg accttaccgg tggcgaagat
     6481 caccagcttc tggcgcaccg tcaggttgcc gtcgaagctg ccgcgcactt cggcgatgtc
     6541 gatctcggcc gagcccttga acgagccctg ctcggcgatc tggatcacgc gcgagtccat
     6601 ggtggcttcg accatgcctt ccaccaccag cgtgtcgcaa tcggtgattt cgacgccctt
     6661 gagcttgatg ttggggccga cgatcagttt ggcgccgctt tcggtgaccg aggccgccac
     6721 cggggtgctg gcgggggcgg ccgggctggc cgccgccgag ggggccgcaa acggctgcgc
     6781 gccggcggcg ccggccgagc ccagggcgct ggtgccgctc agcggcgtac tgccggggcc
     6841 gcggggggtg gtgggttcgt cacgcttgcc gaagaacggt ccttgaagtg ccatgctagc
     6901 tccttgtggt aaacaattga tcctaatgtc ctaagtcgcc aacacgccct acaagaggaa
     6961 agattcgtaa ccaggcgccc cgggggcgcg gtgccggccc gcccatgccg cgtggtcgcc
     7021 ggccacctgc ctggtgcggg cggggccgtt acagtgtctg tccgatcgcc ttgccgccat
     7081 gtccgccgaa ccgccccgca tcgaccacgc gcccgccgac gcggcggcgc gcgtgctgct
     7141 gcgcggcgag tggacggcgg catggctgag cacgcggccg gtctggcgcg ccacccaggc
     7201 ggcgctgggc ggcgtggccg ccggtggcag ctgggacctg cgccagctgg cggcgctcga
     7261 tcacctgggg gcgctggtgc tgtggcacca ctggggccgc cgctggccgg ccgagctgct
     7321 gagcagcgac gagcagcgcg cgctgctgga gcgcgtggcc gccggtgccg cgccggcgcc
     7381 gccggccgag gcgcgcgccg gctggtccga gggggtggag gcgctgggcc tgcgcgtgac
     7441 cgacgccgcc tttcaggtgc gggacttcgt gaccctgatg gggcagctgc tgctggacgc
     7501 gctgcggctg ctggcgcggc ccgggcgcgg gccgtggcgc gacttttccg gccacctgta
     7561 ccgcatcggc gcgcaggcgc tgccgatcac ggcgctggtg ggctttctga tcggcgtggt
     7621 gctggcctac ctgatgagcc aggtgctgcg ccagttcggc gccgaggcct tcatcgtcaa
     7681 catcctgggg ctggcgctga tccgcgagct gggaccgatc ctggcggcgg tgttgctggc
     7741 cgggcgttcc ggctcggcca tcacggcgca gatcggcgtc atgcgcgtga ccgaggagct
     7801 ggacgccatg cgcgtgctgg gcatcccggc ggggttccgg ctggtgctgc cgcgcgcgct
     7861 ggcgctggcc atcgcgatgc cgctgatttc ggtctggacc acgctggcgg cgctggtggg
     7921 cggcatgctg gcgtcggaca tcacgctggg catcacgccg gcgtacttcg tggaggcgct
     7981 gcccaaggcg gtgcgcattc ccaacctgac gctggcggtg tccaagtcgg tggtgttcgg
     8041 ggtgtcgatc gcgctgatcg gctgccacta cggcctgcgc gtcaagccca acaccgaaag
     8101 cctgggccag ggcaccacgg cctcggtggt ggcgtcgatc acggcggtga tcctgatcga
     8161 cgcgctgttc gcggtgctgt tcaagagcgt ggggatatga cgaactcccc tgaaccgctg
     8221 cgcgtcttcc cctctgggag gggggacgca ccccgtggcc ggcgcaggtc cggccacggc
     8281 tgtactggct tggcctgctg cgcggccatt cgatcttcga tgcgtcgcgc catgcgggca
     8341 gcgcgtgcgc cgtggggtgc tggctgatgg aggcggcggt gcaagcgtcc gatgcagtcg
     8401 acaccgcgct ggcgccgggc cagccggtga tcgaggtgcg cgggctgtgg agcgtgtttg
     8461 cgctgcccgg cggcggccag cacgtggtgc accaggacct cgatctgacg gtgcggcgcg
     8521 gcgaggtgct gaccctggtg ggcggctcgg gcaccggcaa gacggtgctg ctgcgccaca
     8581 tgctggggct gacgcggccg gcgcgtggcc aggccacggt gctgggtcgc ccggccggcg
     8641 agctggcgcg cgagggcgcc gcggcgcggg tgggcatgct gtttcagcag ggggcgctgt
     8701 tttcggcctt cagcgtgctc gacaacatcg ccttcgcgct gcgcgagagc ggcgcgctgc
     8761 cgccggcgct ggtgcgcgaa gcggcactgc tgaagctgca gatggtgggc ctgaaggcca
     8821 gcgacgcgca caagatgccg gccgaactgt cgggcggcat gatcaagcgc gtggccttgg
     8881 cgcgcgccct catcatggac ccgccgctgc tgatgctgga cgagcccacc gccgggctgg
     8941 acccggacag ctcggacgcc ttcgtcgccc tgctgcgcgc gctgcaccag gagctggggc
     9001 tgacggtggt gatggtcacg cacgacctgg acacgctgtt cgagctgtcc agccgcgtgg
     9061 ccgtgctggc cgacaagcgc gtcatcatca acgcgccggt gcccgaggtg ctggcgttcg
     9121 agcacccctt catccgccac ttcttcctgg gtgagcgcgg ccggcgcgcc atggagctgc
     9181 tgcgccagcg ccagcacgcg cggcccgcgc aaggagactg attcgatgga aaacaaagcc
     9241 cacgccctgg ccgccggcac cttcgtggtg ctggtcaccg ccctgctcat cgggctggcc
     9301 atgtggctgc tgcgcgacgt caccaacacc cgcgcctacg agatcgtcag ccacaacgcc
     9361 gtcagcggcc tgcagccgca ggcggcggtg cgcttcaagg gcgtggcggt gggcaaggtc
     9421 acgcgcatca gcttcgaccc cgccgagcgc ggccgcgtgc tggtgacgct ggccatcgcg
     9481 cccgacgccc cgatcacccg cgccacggtg gccgagctgg cctaccaggg cgtcaccggc
     9541 ctgtcgttcg tgcagctcga cgacgacggc agcgacgccg cgccgctgcc gcccggcccc
     9601 aacggcgtgc cgcgcatccc gctcaagccg gggctgctgg gtcagctcgg cgatcgcgcg
     9661 caggacctga tcggcaagct cgacaccagc gcccagcgca tcaaccagct gctggcgccg
     9721 gacaaccagg cggtgctgac cggggcgctg ggcgacatcg gcgccgccgc gcgcagcgtc
     9781 gatcggctgg cgcgcaccgc cgaccagacg ctgcaggccc agctggccac cagccgcacc
     9841 agcattccgc gcctgatcac ccaggccacc gacaccctca ggacgctgca ggacgccacc
     9901 accgaggcgc gcaccgtgat ccacaccctg ggccaggcgg cgcaacagac gcagcagggc
     9961 gtggcgcgcg tgaccgcgcc gggcggcgtg atcgaccggc tggatcaggg cgtgcagacc
    10021 atcaacgcca gcacgctgcc gcgcctgcac ggtctggccg acgccgccga ccagaccttg
    10081 cgccgcgtcg atcgcgtggc gcgcaccctg ggcgacaacc cgcaggcgct ggtctacggc
    10141 agtggcccgg tgccgcccgg cccgggcgaa gccggcttcg tgccgccgcc cgcccatccg
    10201 acctctccac gctgagcatt gatatgaaat cgatagctgc tcgcgcacgt cctgcaagcg
    10261 ccagggccgt tttttttgca ttgtctgcgg tggcggcgct ggcgctggcc ggttgcgccc
    10321 tgccgctgcc cgacaagccg ccgcgcaccg agccttacga cctcgggccg ccgccggcgg
    10381 ccggcgccgc cgcgacgccg ggcgcgccgc tggcgctggc gccgatcgag gccagccccg
    10441 ccctcgacag ccaggccatg gtctaccgcc tgctgtacgc cggcgccggc cagcagccgc
    10501 gcccgtatgc gcaggcgcgc tggagcatgc cgccgccgca actgctggcg ctgcgcctgc
    10561 gccagcggct ggcggccacg cgcccggtgc tggacgccgg cagcgcgctg gcggcgctgc
    10621 agctgcgcgt cgagctggac gacttcgcgc acgagttcag cagcccgcag accagcgaag
    10681 gcgtggtgcg gctgcgcgtg accgcccttg ccgccggcgc gcgcgcgcag ccgctgctgg
    10741 gccagcgcag cttcagcgtg cgccggcccg cccccacgcc cgacgccgcc ggcggcgccc
    10801 aggcgctgcg cgacgccacc gacgaggcgc tgcgccagct gatcgactgg ctggccggcc
    10861 tgccggcatc gggttgatct tcaagccgga gcgggcgctg gcgcgcctgc acccagggca
    10921 aacagccagg ttcccatggc tgcggggtct tgaaactttc aaggcatcca agggccgaaa
    10981 ccacacgatg attgcaggag ccaacggatg ggacagacga tcgagctgaa ggccgccgac
    11041 gggcagctgg tgccggccta cctggcgccg cccgcggggc cggcgcgcgg cgccgtggtg
    11101 gtgctgcagg aaatcttcgg cgtcaaccgc cacatccgcg ccgtcgccga cggctttgcc
    11161 ggcgacggct atctggcgct ggcgcccgcc ctgttccagc gcttgcgcgc cagggtggag
    11221 ctgggctacg aggaggccga cgtcaaggcc ggcctggcgc tgaaggcggc ggccgaggcg
    11281 ctgcccgccc ccggcgtgct ggccgacgtg caggccgcca tcgaccacgc ggcgcaggcc
    11341 agcggcggca aggtgggcgt gatcggctac tgctggggtg ggttgctcag ctggcgcgcc
    11401 gccgccctgc tggaccggct ggcggcggcg gtgccctact acggcggcgg catgacgcag
    11461 ccggccgagc tggcgcgcca gccgcgcgtg ccggtgctgg cgcatctgtc ggaccacgat
    11521 gcttcggtgc cgctggacgg cgtggcggcg ctgcgccgcg cgcaccccga ggtcgagctg
    11581 cacctgtacc ctgccgcgca cggcttcaac tgcgaccagc gcgccgcctg gcagcctgcc
    11641 gccgccgcac tggcgcgcga gcgcacgctg gccttcttcg cccggcacct gcgctgatgc
    11701 ggcggcgccc catccgtcat cgggaatcga cccgccgccg ccccggcgcc ggcagcgtgg
    11761 gcgtgggcgg cggctggaac tgcgcatcca ggtagtccag cagggcgcgc agggcggcgc
    11821 tgctgtggcg ccgctgcatg taggccacgt acagccagat ctcggggggt gagtagtcgt
    11881 gcagcagcgg ctccagcgtg ccgctggcca gcgcgtcggc cagccagtgc accgtgcccc
    11941 agaccacgcc caggccctgc agcgcccagg ttctgagcgg cgccgggtcg gtggcggtga
    12001 agcgcggcgt caggcgcagc tccagtggct tgccgcggtc ctggaactgc cagaccgggg
    12061 cttcgccggg cagggcgaag gccagctggt cgtgctccag cagctcgctg gggtgggccg
    12121 ggcgaccgcg ccggtcccag tagtcggggg tggcggcggc cacgtggtcc agccgcagca
    12181 ggcggcgcac gatcaggttg ggctgcagga tcgggccgat gcgaaacgcc aggtccaccc
    12241 cttcgtcgac caggttgacc aggcggttgg tgacgtgcag gtgcagcgtc acttcggggt
    12301 agcgctgcat gaagcggccc agcagggccg gcagttcgct ggccatcagc gcgtgcggca
    12361 cggtcagcgc caggcggccg accgggcggg tggcgcgctc gtgcatctcg ccctcggtga
    12421 agtcgatcag ctccagcagc gcgccgctgc gctcgaacag cagctcgccg gcttcggtca
    12481 gcgtcacctt gcgcgtggtg cgatgaaaca gccgcaggcc gaagcgcgcc tccagctgcg
    12541 cgatgtactt gctgacgttg gccggcgaca tgcccagcgc ccgcgcggcg ccggaaaagc
    12601 tgccgcgccg cgcgacttcg cggaaggtct ggatcaggtc gagcgagtcc atgtgttgcc
    12661 tggccagcgc gcgcctcagc gcgtcaggta gcggcggcgc cagaacagca ccacgatgcc
    12721 cagcgccagc accaccagca gcagcagcgc cagccacatg ccttccacgc tgtggatcag
    12781 cggcaggtgt tcgaaattca tgccgaagaa gccggtgaac aggttcagcg gcaggaacac
    12841 cgcggtgatg gcggtcagcg cacgcatggt gtcgttggtg cgctgaccct gggcggcaaa
    12901 gtggatctgc accgcggttt cggccgcctg ttccagccgg cgtgcgtggc gcagcacgcg
    12961 ctcgatgtgc tccatgacgt cgcgtgcgcg cgccaccagc tggtcgtggc gctgctgggc
    13021 cagcagcggg tcggggccat aggtggacag cggcagctcg cgcaaggtgt ccagccattc
    13081 ctgcatggcg tcctgctgct cctcgcacag gtcctgcagc ccgtgcaggc gcccgcgtgc
    13141 gtgcatcagc gcgccccagg tgtgcggcgc cgggtgcggc ttgagcagct cgacctgcgc
    13201 ctgctccagc gcttcggaga ggtcgcgccg caggtccagg tagctgtcga ccatggcgtt
    13261 gagcatgcgc agcaccaggt cgtccggccc ctgcggcacg cggttgcggg cgctggcgct
    13321 gtccacgctc aggcgcgcgt cgtcggcctg gcgctgcaga aagccgaccg ccgtctggca
    13381 gcccggcggg tgcacgctga tcagcaggcg gtcgaagacg acgaagccga ccgcgcgcga
    13441 atcgatgcgc tcgaacggct gcggcttggg cgcgcgcggc gcgccggccg cgccggtgtg
    13501 ggggtcgtgg ttgccgttga gctgcacctc ggccagcgac gccaggcgtc ggaacaccac
    13561 caggtcgtac accgaggtgg cgtcgtagcc cgaagggtgg gccgggttgg ccaggtcctt
    13621 ggcgtgcacg tccagcagcg gcgagccgcc caggcggtgc gccgcctctt gcagctcggg
    13681 caggtggcgg gcaaagtcct cgcggtccag gtagatccag acgaagccgt cggccggcgc
    13741 cgccgccggc acggcgtcgg caaagcgcag gctgccgccg gtgaattcga cgatgtgcac
    13801 gacggccccc tccgaatcgt ggtcaaaatg gcttctggcg cgctatggac aagcgccagt
    13861 agctattgat tttgtagtaa acgggcggca tcgcgggcaa agtaggtcag cacgccgtcg
    13921 gcgccggcgc gcttgaaggc gagcaggctt tccatcatca cccggtcgtg gtccagccag
    13981 ccgttttgcg ccgcagcctt gagcatggcg tattcgccgc tgacctggta ggcgaaggtg
    14041 ggcacgccga actggtcctt gacgcggcgc acgatgtcca ggtagggcag gccgggcttg
    14101 accatcacca tgtcggcgcc ttcggcgatg tcgatggcga cttcgcgcag cgcctcgtcg
    14161 ctgttgccgg ggtccatctg gtagaccttc ttgtcgctgg cgcccaggtt cttggccgag
    14221 ccgaccgcgt cgcgaaacgg gccgtagaac gcgctggcgt atttggcgct gtaggccatg
    14281 atgcgggtgt ggatgtggcc ttttttctcc agcgcgtcgc ggatggcgcc gatgcgcccg
    14341 tccatcatgt cgctgggcgc cacgatgtcc acgccggccg ccgcctgcgt cagcgcctgc
    14401 ttgaccagct ggcccacggt ctcgtcgttg aggatatagc cggtcttgtc cagccagccg
    14461 tcctggccgt ggctggtgta ggggtcgagc gccacgtc
//
LOCUS       DAOOOG010000048        14294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4573_length_14294_cov_23.212234, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000048 DAOOOG010000000
VERSION     DAOOOG010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14294
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4573_length_14294_cov_23.212234"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1062
                     /locus_tag="PK242_05495"
     CDS             118..1062
                     /locus_tag="PK242_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ93504.1"
                     /translation="MIAPPLLDVQDLSKSYAGTRVVDGVSLTIAPGECLGVIGPNGAG
                     KTTTIRLCLGLTAPDAGRIRYFVQPGGGALEMPRDALAIKARLGVVSQFDSLDPDFDC
                     AENLHVFGRYFRLARAELAGRVPQLLEFAALTHKARARPGELSGGMRRRLSLARALIN
                     DPELLLLDEPTTGLDPQARHLMWERLQRLLADGKSILLTTHFMDEAERLCHRLLVIDH
                     GRKIAEGRPRELIAAHLEPEVVEVYGAGALALAQAPLTRHAERVEHSGETVFFYTRDA
                     RPLMQALQAWPQLRTLHRPANLEDLFLKLTGRQIRQEG"
     gene            1100..1900
                     /locus_tag="PK242_05500"
     CDS             1100..1900
                     /locus_tag="PK242_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ93505.1"
                     /translation="MATSSIWAAPRASMRWWPVFARNLRVWRKLAIPSLLANIGEPLL
                     WLVAFGYGLGAMVGRVPTHGQDVPYILFLASGSVCMSAMNAASFEALYSAFSRMHVQK
                     TWDGILNTPVQLDDVVLAEMLWAGFKALFSAAVILAVIWALGISRAPRTLLALPVLLA
                     TGITFASLALVFNAKASGYDFFTYYFAVFLSPMMFLSGVFFPRQQLPAFAQHITDWLP
                     LTAAVELVRPLFLDQWPAQPWRHAAVLLVYAVLGYWVALAFTRQRFRK"
     gene            1925..2416
                     /locus_tag="PK242_05505"
     CDS             1925..2416
                     /locus_tag="PK242_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93506.1"
                     /translation="MSPLLSPAHIALIDQGVSVIVASRDAQLRPSVMRAMGSQISADG
                     SCITVYLRPSQAQPLLADLAAGGPIAVVFSDPASNRTLQVKAGAARARAASPADQAVL
                     RRYRGSMQQRIGQVGFGADYVAAMLAAPADDLVALEFTPEAAFDQTPGPRAGRPVGVG
                     AKP"
     gene            2413..3783
                     /locus_tag="PK242_05510"
     CDS             2413..3783
                     /locus_tag="PK242_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOZ93507.1"
                     /translation="MSGAANPTGAAPAPTLRSIRACLEGAIPANIATCAPDGTPNVAY
                     LSQVVYVDEGHVALSFQFFNKTRQNILANPCATVLLLDPVTAQYYRLQLRYLRTETEG
                     PLFERMRAQLAGIASHEGLAEVFALKGSDVYAVEQIEALASELLPPAPPRADLLGAVR
                     LCSLRLARCTGLDELLQALLAGLSEHLGIAHAMVLMLDGAAGQLYTVASMGYARSGVG
                     SEIRLGQGVIGMAAREATAVRIGHVTHAALYNHGLRHSLEADGTAAGPEIPYPGLAEP
                     HSQLAVPLISGGRTLGVLFVESPLDLQFRYPEEDALVTLAGQLAAAIDRLQVPDDSSE
                     PARPYASRTPPQGPPLRVRHFAADGSVFVNDSYLIKGVAGAILCKLLREHASSGRQEF
                     SNRELRLDRTLGLPEVADNLEARLLLLQRRLQESGTGIRIDRAGRGRFRLHLDAPVLL
                     DPRSGS"
     gene            complement(4029..4745)
                     /locus_tag="PK242_05515"
     CDS             complement(4029..4745)
                     /locus_tag="PK242_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93508.1"
                     /translation="MQLDSQELAALAQAAVTPRMDMYAFIHKALRAYMSDTLVALGRV
                     DVNDEPELGQICDRVRQLLDFCRAHLRHESDFIHPAMERHLPGSAAAVAQEHVGHVQA
                     IATLADGTAQLVACAPERRAPLAHALYRQLALFVAHNFEHMQQEETQHNAVLWAHYSD
                     AELVAIHDRLVASIPPEEMGVVLRWMLPHLTPDERAMMLADMRRSAPPPAFEAALALA
                     RAQLTPREWDKLARALELAP"
     gene            complement(4864..6450)
                     /locus_tag="PK242_05520"
     CDS             complement(4864..6450)
                     /locus_tag="PK242_05520"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HOZ93509.1"
                     /translation="MDYVIIGGGSAGCVLAARLTEDPGVNVTLLEAGPPDSSVLIHCP
                     GGLAAVAKFELFGWGQNTVPQPGLGGRRGYQPRGKVLGGSSSVNAMIYIRGQHADYDH
                     WAAQGNPGWGWQDVLPYFLRAEHNERGASAWHGQGGPFNVMDLRSPNRFAQVFVDAAV
                     QAGFEHNPDFNGATQEGVGLYQVTHRNGERHSTAKGYLAQALGRPNLQVITGAHATRV
                     LLEGRRAVGVEYRQGGQLKQLRVRREVLLSGGALLSPQLLMLSGIGPSDELQRHGIGV
                     LHHLPGVGQHLHDHPDVVQVVDAPQLTELFGLSLRGGLNVLRGMREWRTQRRGMLTTN
                     FAEAGGFIRSRPEEDAPDLQLHFVIGKLVDHGRKTVFGHGYSCHVCVLQPQSRGSVRL
                     ASNDPLALPLVDPNFLGHADDMARMVRGFQITRRILQQPALAQYGGRETASLAAAQTP
                     AEIEQFIRRYADTIYHPVGTCRMGPGPSDVVDSQLRVHGLAGLRVVDASIMPRIVSGN
                     TNAPTVMIAEKAADMIRQAA"
     gene            6611..7462
                     /locus_tag="PK242_05525"
     CDS             6611..7462
                     /locus_tag="PK242_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOZ93510.1"
                     /translation="MNISTVGIVGAGTMGNGIAQACAVSGIQVVMVDIAQAAVDKGLA
                     TIGKSLERLLQKDKISEADKTAALARIQGSTDYDALKGAQLVIEAATENHELKNKILK
                     QLDALLAPEVIIATNTSSISITQLAAVTQRPERFVGMHFFNPVPMMALVEIIRGLQTS
                     DATHDAVKALAVKLGKSPITVKNAPGFVVNRILVPMINEAFFVLAEGLATPEDIDAGM
                     RLGCNHPIGPLALADMVGLDVCLAVMDVYLKEFGDSKYRACPLLREMVAAGHLGRKTG
                     RGVYSYA"
     gene            complement(7515..9674)
                     /locus_tag="PK242_05530"
     CDS             complement(7515..9674)
                     /locus_tag="PK242_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOZ93511.1"
                     /translation="MAQIGDKTEVRTEQVQARLLAHAPQGVPVGLAADAAARQPVWLG
                     LQITHAPDWHTYWKNAGDSGLPTELQWTLPPGVLAGDIAWPLPRKIPIGHLANYGYEG
                     TVLLPVPLTITPDYQPPPLADSLEIGLKASWLVCRQECIPQEGEFSLRLPLRSSTALH
                     GAAFEAALARQPTDIGGAQQLTLTEGGRRLTLRVSGLPAAARGQVLELFPETPNIVVT
                     AARLAAPGEALGERSWRQQWDGDVWTADIPVSPDRAEAPTRMPWVLVAGERGWRVTAP
                     VSGDWPALAAAVGVSPALEAALAANTARADGAAPPAATPAPVGTLVLALLGALIGGLV
                     LNLMPCVFPVLAIKVLGFTQHAEDRRAHRLGGLAYTLGVVLSFLALGGLMLALRAAGQ
                     QLGWGFQLQSPLVVALLAALFTVIGLNLAGVFEFGRFVPSSVATLEARHPMANAFLSG
                     MLAVAVASPCTAPFMGASLGLAVGLPAGQALAVFGAIGLGMALPYLAASWLPALARLL
                     PRPGAWMEVFRRFMAFPMFGTTVWLLWVLGQQTGIDGAGALLALLVLLALVLWALGLT
                     GRARAWVATIAVASGAFLVWSVGPYVVRPLAEPEPAGVAAGERWAPWSPARVQARLAE
                     GRPVFVDFTAAWCVTCQYNKKTTLASAEVLDAFDRAGVQTLRADWTRRDAGISAELGR
                     LGRSGVPVYLLQAPGKPPVVLSEVLSVADVMAALAQL"
     gene            9969..11222
                     /locus_tag="PK242_05535"
     CDS             9969..11222
                     /locus_tag="PK242_05535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ93512.1"
                     /translation="MRLSTPSSLERTLTPWADRLRERLDLPLLVRWQGGGGLRLGWFE
                     QPRVTLEVRDAIGATALLAPSLDNLGRAYVEGHIDVDGRVQDIIDVAHRLAEAGARHD
                     GGAGWVGRAAQRMADAVGHTRAQDRAAIQHHYDVSNDFYAAWLDPAMVYSCAYFETGT
                     ETLAAAQQRKIDHILTKLRLRPGQTLLDIGCGWGALVLRAAQQFGARCVGITLSQNQH
                     ALARERVQAAGLADRVDIRLQDYRDVRGRFDRITSVGMFEHVGLKNLPGYFRILHDLL
                     APDGWALNHGITSTDPDNGETRHGGGRFIDRYVFPQGELPHIATVLTTLQQGGLEPVD
                     VEGLRRHYARTLQCWSEAFEARAEHLKTLVDEKTWRVWRMYLVGSQWAFEHDEISLFQ
                     VLCRRAGQPAATLPWSRRWMYKAAH"
     gene            complement(11339..11824)
                     /locus_tag="PK242_05540"
     CDS             complement(11339..11824)
                     /locus_tag="PK242_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOZ93513.1"
                     /translation="MSRRSALWWAAGAAMLLAGGLLAWRAWWPGSAASLLRPDDPAVL
                     ALGQRVYAAHCASCHGVHGEGQPRWREPGPDGLRPAPPHDASGHTWHHPDAQLLAITR
                     HGLARVIGQPGYRSAMPAYDGVLSDAEIVAVLSWIKSRWPPAVRAQHDAVNAQARDAG
                     G"
     gene            complement(12201..12692)
                     /locus_tag="PK242_05545"
     CDS             complement(12201..12692)
                     /locus_tag="PK242_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HOZ93514.1"
                     /translation="MQKSHPRRRQALRLLALAAAAALVPGVQAQRRPQPIAVEVWKDA
                     SCGCCHDWIAHMQQHGFVVTAHDTGNNAVRARLGLAARYGSCHTALVGGYVVEGHVHA
                     DDVHRLLRDKPRALGLAVPGMPVGSPGMDGPAYGGRRDSYDTLLVQRDGSSQVFRRHG
                     GPS"
     gene            12826..13419
                     /locus_tag="PK242_05550"
     CDS             12826..13419
                     /locus_tag="PK242_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93515.1"
                     /translation="MTAHPRASPTRLALGLSAACALLLGLLAAICLLALQLHFESRDR
                     KQLQSHLHTTQALLASVDNAAALAALPARLAGALPGPQLLAVRVQGALGQPLYEQGAH
                     TAMPASLLAQPGVALPIALHSWQHDGHHWRGSALRMRLPLDGAAPLTVAMALDVQPQA
                     RFLASLRWALVGYVLLAALLFGLLARWWLGRTLARGH"
     gene            13466..>14294
                     /locus_tag="PK242_05555"
     CDS             13466..>14294
                     /locus_tag="PK242_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOZ93516.1"
                     /translation="MTKLPPRYPRHRQWSDLTTTDFASLDLARAIAVLPLGATEQHGP
                     HLPLSVDGNLAHGMVQAATPHLPIDLPALFLPVQRIGHSPEHAAFAGTLSLKADTVIR
                     LWTDIAESVHASGVRKLVLFNAHGGNVGLMDVVARELRARLSMLVYSVSWFNLPLHDA
                     EGNDLLARVAPEEQRFGVHGGQLETAMMLGLKPRLVRKHKAQDFASSSRERAEGFAIL
                     GNGRSAKLAWAAQDLNPAGVTGNAAAATVEDGLALVDAAGRALAALLQEIDRLPADTL
                     "
BASE COUNT         2094 a         5204 c         4931 g         2065 t
ORIGIN      
        1 ttccttcggg gggtccgtca cccgccgtgg ctgcgcccgc agcccggggt cacgggaaat
       61 gaaaggcgga tttttaccac gacgccgggc gccggcgcgc gtgcacaatc gccgcccatg
      121 atcgccccgc cactgctcga cgtgcaggac ctgagcaaga gctacgccgg cacacgcgtg
      181 gtggacggcg tgtcgctgac catcgccccc ggcgaatgcc tgggcgtgat cggccccaac
      241 ggcgccggca agaccaccac catccgcctg tgcctgggcc tgacggcgcc ggacgccggc
      301 cgcatccgct acttcgtgca accgggcggc ggcgcgctgg agatgccgcg cgacgccctg
      361 gccatcaagg cacggctggg cgtggtgtcg cagttcgact cgctcgaccc ggacttcgac
      421 tgcgcggaga acctgcacgt gttcggccgc tacttcaggc tggcgcgcgc cgagctggcg
      481 gggcgggtgc cccagttgct ggaattcgcc gccctcaccc acaaggcccg cgcccggccg
      541 ggcgagctgt cgggcggcat gcgccggcgc ctgtcgctgg cgcgcgccct catcaacgac
      601 cccgagctgc tgctgctgga cgaacccacc accggcctcg acccgcaggc gcgccacctg
      661 atgtgggagc gtctgcagcg cctgctggcc gacggcaagt cgatcctgct gaccacccat
      721 ttcatggacg aggccgaacg cctgtgccac cgcctgctgg tgatcgacca cggccgcaag
      781 atcgccgaag ggcgtccgcg cgagctgatt gccgcccacc tggagcccga agtggtcgag
      841 gtgtatggcg ccggcgcgct ggcgctggcc caggcgcccc tgaccaggca cgccgagcgg
      901 gtcgagcaca gcggcgagac ggtgttcttc tacacccgcg acgcgcggcc gctgatgcag
      961 gcgctgcagg cctggccgca gctgcgcacc ctgcaccgcc cggccaacct cgaagacctg
     1021 tttctcaagc tcaccgggcg ccagatccgg caggagggct gagaagcagt gaccgaattc
     1081 ctgttgcccc cgttctgaca tggccacctc atccatctgg gcagcgcccc gcgcatcgat
     1141 gcgctggtgg ccggtgtttg cgcgcaacct gcgggtgtgg cgcaagctgg ccatccccag
     1201 cctgctggcc aacatcggcg agccgctgct gtggctggtg gcgttcggct acgggctggg
     1261 cgccatggtc gggcgcgtgc ccacccacgg gcaggacgtg ccctacatcc tgtttctggc
     1321 cagcggctcg gtgtgcatga gcgccatgaa cgcggccagc ttcgaggccc tgtattcggc
     1381 cttctcgcgc atgcatgtgc agaagacctg ggacggcatc ctcaacaccc ccgtgcagct
     1441 cgacgacgtg gtgctggccg aaatgctgtg ggccggcttc aaggcgctgt tctcggccgc
     1501 cgtgatcctg gcggtgatct gggcgctggg catcagccgc gcgccgcgca ccctgctggc
     1561 gctgcccgtc ctgctggcca ccggcatcac cttcgccagt ctggcgctgg tgttcaacgc
     1621 caaggccagc ggttacgact tcttcaccta ctactttgcg gtgttcctgt cgcccatgat
     1681 gttcctgtcg ggcgtgttct tcccgcgcca gcagctgccc gccttcgccc agcacatcac
     1741 cgactggctg ccgctgacgg cggcagtgga gctggtgcga cccctgttcc tcgaccagtg
     1801 gccggcgcag ccttggcgcc atgcggcggt gctgctggtg tacgcggtgc tgggctactg
     1861 ggtggccctg gccttcacgc gccagcgctt tcgcaaataa tccgggcgtt tgcgctgcac
     1921 acccatgtcg cccctgctga gcccggcaca catcgccctg atcgaccagg gcgtgtcggt
     1981 catcgtcgcc tcgcgcgacg cgcagctgcg ccccagcgtg atgcgcgcca tgggttcgca
     2041 gatcagcgcc gacggcagct gcatcacggt ctacctgcgc cccagccagg cgcagccgct
     2101 gctggcggat ctggcggccg gcggcccgat cgccgtggtg ttcagcgacc cggcgtccaa
     2161 ccgcacgctg caggtcaagg ccggcgccgc ccgcgcgcgt gcggcgagcc cggccgatca
     2221 ggcggtgctg cgccgctacc gcgggtcgat gcagcagcgc atcgggcagg tcggctttgg
     2281 cgcggactac gtggccgcca tgctggcggc gcccgcggac gacctggtgg cgctggaatt
     2341 cacgcccgag gcagccttcg atcagacgcc cgggccccgc gccggccggc ccgtgggtgt
     2401 gggtgccaag ccatgagcgg cgcggcgaat cccaccggcg ctgcgcccgc gcccacgttg
     2461 cgctccatcc gcgcctgcct ggagggcgcc atcccggcca acatcgccac ctgtgcgccc
     2521 gacggcacgc ccaacgtggc ctatctctcg caagtggtgt acgtggacga gggccatgtg
     2581 gcgctgtcgt tccagttctt caacaagacg cgtcagaaca tcctggccaa cccctgcgcc
     2641 acggtgctgc tgctcgaccc ggtgacggca cagtactacc gcctgcagct gcgctatctg
     2701 cgcaccgaaa ccgagggccc gctgttcgag cgcatgcggg cgcagctggc gggcattgcc
     2761 tcgcacgagg gcctggcgga ggtgtttgcg ctcaagggct cggacgtcta cgcggtggag
     2821 cagatcgagg cgctggccag cgagctgttg ccccccgcac ccccacgcgc cgacctgctg
     2881 ggcgcggtgc gcctgtgcag cctgcgtctg gcgcgctgca ccggactgga cgaactgctg
     2941 caggccctgc tggccggcct gagcgaacac ctggggattg cgcacgccat ggtgctgatg
     3001 ctggatggcg ccgcgggcca gctgtacacc gtggccagca tgggttacgc cagatcgggc
     3061 gtgggcagcg aaatccgtct gggccagggc gtgatcggca tggccgcgcg tgaggccacg
     3121 gcggtgcgca ttggccacgt cacgcatgcg gcgctctaca accacggcct gcgccacagt
     3181 ctggaggccg atggcacggc cgccggtccg gagattccct accccggatt ggccgagccg
     3241 cacagccagc tggcggtgcc gctgatcagc ggcggccgca cgctgggcgt gctgttcgtc
     3301 gaaagcccgc tggatctgca gtttcgctac cccgaggagg acgctctggt cacgctggcc
     3361 ggccagctgg ctgccgccat cgaccggctg caggtgcccg acgattcgag cgagccggcg
     3421 cgcccctatg cctcgcgcac gccacctcag ggcccgccac tgcgggtgcg ccactttgcc
     3481 gccgacggca gcgtgttcgt caacgacagc tacttgatca agggcgtggc cggcgccatc
     3541 ctgtgcaagc tgctgcgcga acacgccagc agcggccggc aggagttcag caaccgcgag
     3601 ctgcgcctgg atcgcacgct gggcttgccc gaggtggccg acaacctgga ggcccgcctg
     3661 ctgctgctgc agcgccggct gcaggaaagt ggcaccggca tccgcatcga cagggcgggg
     3721 cgcggacgct ttcgactgca cctggacgcg ccggtgctgc ttgatcctag aagcggcagt
     3781 tgaccgcttg tcaacttcgg gcagaccgca tcgatgcggg gactcgcggc tgcgatggcc
     3841 ttcacctcct gggtgagcac gctacgcacc cacccgcacc gcgctcgccg cgccatgcgg
     3901 cgttgctcct tcttgcgggc accagcccgc ggcgtcgtcg cgtcttgcct ggcacgccga
     3961 tcacggcacg ggcgggcgcg tccaactgcc gtttctaggt tgaatcggtg ccgggctgaa
     4021 ggtccgcgtc agggcgccag ttcgagcgca cgcgccagct tgtcccattc gcgcggggtc
     4081 agctgcgccc gcgccagcgc cagcgcggct tcgaaggccg gcggcggcgc gctgcggcgc
     4141 atgtcggcca gcatcatggc gcgttcgtcc ggcgtcaggt ggggcagcat ccagcgcagc
     4201 accaccccca tctcctccgg cgggatggag gccaccagcc ggtcatggat ggccaccagc
     4261 tcggcgtcgc tgtagtgggc ccagagcacg gcgttgtgct gcgtctcttc ctgctgcatg
     4321 tgctcgaagt tgtgcgccac gaacaaggcc agctgccgat acagcgcgtg agccagcggc
     4381 gcgcgccgct cgggcgcgca ggccacaagc tgcgccgtgc cgtccgccag tgtggcgatg
     4441 gcctgcacat gcccgacatg ctcctgtgcc accgcagcgg cgctgcccgg caggtggcgc
     4501 tccatggcgg ggtgaatgaa gtcgctttcg tgcctgaggt gtgcgcggca gaagtcgagc
     4561 agctggcgca cgcgatcaca gatctgaccg agttcgggct cgtcgttcac gtccacgcgg
     4621 cccagcgcca ccagcgtgtc ggacatgtac gcgcgcaggg ccttgtggat gaaggcgtac
     4681 atgtccatgc gcggtgtgac cgcagcctgc gccagggcgg ccaattcttg ggaatccagt
     4741 tgcatcgtcc attccttgtg ggttgccggc aaaccatatg ccagtccacg aagtctagga
     4801 acgaggccgc cgcggcgctg ctaaaaagcg cttacgccaa ccctcaaaat ttcttaaggc
     4861 gcctcaggcg gcctgacgga tcatgtccgc ggccttctcg gcgatcatca ccgtcggtgc
     4921 gttggtgttg ccgctgacga tgcgcggcat gatggaggca tcgaccacgc gcagcccggc
     4981 caggccgtgc acgcgaagct ggctgtccac cacgtcggac ggccccggtc ccatgcggca
     5041 ggtgccgacc gggtggtaga tggtgtcggc ataacggcgg atgaactgct cgatctcggc
     5101 cggcgtctgc gcggcggcca ggctcgccgt ctcgcggcca ccgtactgcg ccagcgccgg
     5161 ctgctgcagg atgcggcgcg tgatctggaa cccgcgcacc atgcgcgcca tgtcgtcggc
     5221 gtgaccgagg aagttgggat cgaccagcgg cagcgccagc gggtcgttgc tggccaggcg
     5281 cacgctgccg cggctttgcg gctgcagcac gcacacatgg cacgagtagc cgtgaccgaa
     5341 caccgtcttg cggccgtgat cgaccagctt gccgatgacg aagtgcagtt gcaggtcggg
     5401 cgcgtcttcc tccggccggc tgcggatgaa gccgccggct tcggcgaaat tggtggtcag
     5461 catgccgcgg cgctgggtgc gccattcgcg catgccgcgc agcacgttca gcccgccacg
     5521 cagcgacaga ccgaacagct cggtcagctg cggcgcatcg accacctgca ccacgtcggg
     5581 gtgatcgtgc aggtgctggc ccacgcccgg caggtggtgc agcacgccga tgccgtggcg
     5641 ctgcagttcg tccgagggcc cgatgcccga cagcatcagc agctgcggtg acagcagcgc
     5701 cccgcccgac agcagcacct cgcgccgcac gcgcagctgc ttgagctgcc cgccctggcg
     5761 gtattccacc cccacggcac gccgcccttc cagcagcacg cgcgtggcgt gcgcgccggt
     5821 gatgacctgc aggttgggcc gccccagcgc ctgggccaga tagcccttgg ccgtgctgtg
     5881 gcgctcgccg ttcctgtggg tgacctggta caggcccacg ccctcctggg tggcgccgtt
     5941 gaagtcgggg ttgtgctcga agccggcctg caccgcagcg tcgacgaaca cctgggcgaa
     6001 gcggttgggc gagcgcaggt ccatcacgtt gaacggcccg ccctggccgt gccaggcgct
     6061 ggcgccgcgt tcgttgtgct cggcgcgcag aaagtagggc agcacgtcct gccagcccca
     6121 gcccgggttg ccctgtgcgg cccagtggtc gtagtcggcg tgctggccgc ggatgtagat
     6181 catggcgttg acggaactgg agcctcccag caccttgccg cgcggctggt agccgcgccg
     6241 cccgcccagg cccggctgcg gcaccgtgtt ctggccccag ccgaacagct cgaacttggc
     6301 caccgccgcc agaccgccgg ggcagtggat caacacgctg gagtcgggcg ggccggcttc
     6361 cagcagcgtg acgttcacgc ccgggtcttc cgtcagtcga gccgccagca cacaaccggc
     6421 cgagccgcca ccgatgatca cgtaatccat cttgccaagc ctcctgtgac agacattgtg
     6481 cgtgtcgaaa cgccacccgt ttaggtgctt tcctccaaaa aaccggctca cccgccgccc
     6541 tcgttaccat cgtgccatgg cggccgcgcc gtcggccccg catttccaat ccactcacgg
     6601 agtattcagc atgaacatca gcaccgtcgg catcgtaggc gccggcacca tgggcaacgg
     6661 catcgcccag gcctgcgccg tctcgggcat tcaggtcgtc atggtcgaca tcgcgcaggc
     6721 cgcggtcgac aagggcctgg ccaccatcgg caagagcctg gagcgcctgc tgcagaagga
     6781 caagatcagc gaggccgaca agacggcggc gctggcccgc atccagggtt cgaccgacta
     6841 cgacgcgctc aagggcgccc agctggtgat cgaggccgcg accgagaacc acgagctgaa
     6901 gaacaaaatc ctgaagcagc tcgacgccct gctggcgccc gaggtgatca tcgccaccaa
     6961 cacctcgtcg atctcgatca cccagctggc cgccgtgacg cagcgccccg agcgcttcgt
     7021 cggcatgcac ttcttcaacc cggtgccgat gatggcgctg gtggaaatca tccgcggcct
     7081 gcagacctcg gacgccacgc acgacgccgt caaggcgctg gcggtgaagc tgggcaagtc
     7141 gcccatcacg gtcaagaacg cgccgggctt cgtggtcaac cgcatcctgg tgccgatgat
     7201 caacgaggcc ttcttcgtgc tggccgaggg cctggccacg cccgaggaca tcgacgccgg
     7261 catgcgcctg ggctgcaacc acccgatcgg cccgctggcc ctggccgaca tggtgggcct
     7321 ggacgtgtgc ctggcggtga tggacgtcta cctgaaggaa ttcggcgaca gcaagtaccg
     7381 cgcctgcccg ctgctgcgcg aaatggtggc ggccggccac ctggggcgca aaaccgggcg
     7441 cggcgtctac agctacgcct gagcgggtgc gagcgaaccc tcacgcccac cttccgggcc
     7501 tgaacgcccg gccgtcagag ctgggccagc gccgccatca cgtcggccac actgaggact
     7561 tcgctcagca caacgggcgg cttgccgggt gcctgcagca ggtacaccgg cacgccgctg
     7621 cgtcccagcc tgcccagctc ggcgctgatg ccggcgtcgc gccgcgtcca gtcggcgcgc
     7681 agggtctgca ccccggcccg gtcgaaggcg tccagcacct cggcgctggc cagggtggtc
     7741 ttcttgttgt actggcaggt cacgcaccag gcggcggtga agtcgacgaa caccggccgc
     7801 ccctcggcca gccgggcctg cacccgcgcc ggcgaccagg gcgcccagcg ctcgccggcc
     7861 gccacgccgg cgggctccgg ctcggccagc ggacgaacga cgtaagggcc tactgaccac
     7921 accaggaaag cgccagaagc tacagcaata gtagcaaccc aggcgcgtgc gcggcccgtg
     7981 agaccgagcg cccacagcac cagcgccagc agcaccagca gcgccagcag cgcgccggcg
     8041 ccgtcgatgc cggtttgctg gcccagcacc cacagcagcc agaccgtggt gccgaacatc
     8101 gggaacgcca tgaagcgccg gaacacctcc atccaggcgc ccggccgcgg cagcaggcgc
     8161 gccagcgccg gcagccagct ggccgccagg tagggcagcg ccatgcccag gccgatggcg
     8221 ccgaacaccg ccagcgcctg tcccgccggc agccccaccg ccagccccag cgacgcgccc
     8281 atgaacggcg ccgtgcaggg cgaggccacg gccaccgcca gcatgcccga caggaaggcg
     8341 ttggccatcg ggtggcgcgc ttccagcgtg gccacgctgc tgggcacgaa acggccgaac
     8401 tcgaacaccc cggccagatt caggccgatc acggtgaaca gcgccgccag cagcgccacc
     8461 accagcggcg actgcagctg aaagccccag cccagctgct ggccggccgc gcgcagcgcc
     8521 agcatcagtc cgcccagcgc cagaaacgac agcaccaccc ccagcgtgta ggccaacccg
     8581 cccagccggt gcgcgcgccg gtcttcggcg tgctgggtga agcccagcac cttgatggcc
     8641 agcaccggaa acacgcaggg catcaggttg agcaccaggc cgccgatcag cgcgcccagc
     8701 agggccagca ccaaagtgcc gacaggcgcg ggcgtggcgg caggcggcgc ggcgccgtcc
     8761 gcgcgggcag tgttggctgc cagcgccgct tccagcgcag gcgacacgcc caccgccgct
     8821 gccagcgccg gccagtcgcc gctgaccggt gccgtgacac gccagccgcg ctcgcccgcc
     8881 accagcaccc agggcatccg ggtgggcgcc tcggcgcggt cgggcgacac cgggatgtcg
     8941 gccgtccaga catcgccgtc ccattgctgg cgccagctgc gctcgcccag cgcctcgccg
     9001 ggcgcagcca gccgggcggc ggtgacgacg atgttgggcg tctcgggaaa cagctccagc
     9061 acctgcccgc gggcggccgc cggcagcccg ctgacgcgca gtgtcaggcg gcggccgcct
     9121 tcggtcaggg tgagctgctg cgcgccgccg atgtcggtcg gctggcgggc cagcgccgct
     9181 tcgaaggccg cgccgtgcag tgcggtggag ctgcgcaacg gcagcctgag gctgaattcg
     9241 ccttcctgcg gaatgcattc ctggcggcac accagccagc tcgccttcag cccgatctcc
     9301 agactgtcgg ccagcggcgg cggctggtag tcgggcgtga tggtgagcgg caccggcagc
     9361 agcaccgtgc cttcgtagcc gtagttggcc aggtggccaa tggggatctt cctgggcagc
     9421 ggccaggcga tgtcgccggc cagcacgccg ggcggcagcg tccactgcaa ctcggtcggc
     9481 agacccgaat cgccggcgtt cttccagtag gtgtgccagt cgggcgcatg ggtgatctgc
     9541 aggcccagcc agaccggctg acgggccgcc gcgtcggccg ccagccccac cggcacgcct
     9601 tgcggcgcgt gcgccagcag gcgcgcctgc acctgctcgg tgcggacttc ggtcttgtca
     9661 ccgatctggg ccatggcgct tgtctggcaa gcgccgatag ctatcaataa aatagcacac
     9721 agcgaggtca ggaaggggcg caggcgggac atgggaaagc gcaggttgga caggtgaccc
     9781 ccgtgggagg cggggcgccg gcagaggttc cgcgccggcc ctgcgactgt agcccagtgc
     9841 gcccgaacct gccgttgaac ggcaggcggc aatcgtggta atttgcaccc ctacctcgga
     9901 caacgcgcgg ggctgatcgg tcgcctcatg gggcctgcca agccgccgcg ggaaggagct
     9961 gacaagccat gcgtctgtcg acaccgtcct ccctggagcg caccctgacc ccatgggccg
    10021 atcgcctgcg cgagcggctc gacctgcccc tgctggtgcg ctggcagggc ggcggcgggc
    10081 tgcggctggg atggttcgag cagccgcgcg tgacgctgga ggtgcgcgac gccatcggtg
    10141 ccaccgccct gctggccccc tcgctcgaca acctgggccg cgcctatgtg gaaggccaca
    10201 tcgacgtcga cggccgggtg caggacatca tcgacgtggc gcaccgcctg gccgaggccg
    10261 gcgcccgcca cgacggcggt gctggctggg tcggccgtgc ggcgcagcgc atggccgacg
    10321 cggtgggcca cacgcgggcg caggaccggg cagcgatcca gcaccactac gacgtctcca
    10381 acgacttcta tgccgcctgg ctggacccgg ccatggtgta ttcctgcgcc tacttcgaaa
    10441 ccggcaccga gaccctggcc gcggcgcagc agcgcaagat cgaccacatc ctgaccaagc
    10501 tgcgcctgcg gcccggccag acgctgctgg acatcggctg cggctggggc gcgctggtgc
    10561 tgcgcgcggc gcagcagttt ggcgcccgct gcgtgggcat cacgctgtcg cagaaccagc
    10621 atgccctggc gcgcgagcgc gtgcaggccg ccggcctggc cgaccgggtg gacatccgcc
    10681 tgcaggacta ccgcgacgtg cgcggccggt tcgaccgcat caccagcgtc ggcatgttcg
    10741 aacatgtggg cctgaagaac ctgcccggtt acttccgcat cctgcatgac ctgctggccc
    10801 ccgacggctg ggcgctcaac cacggcatca cctcgaccga cccggacaac ggcgaaacgc
    10861 gccatggcgg cggccgcttc atcgaccgct acgtgttccc gcaaggtgag ctgccgcaca
    10921 tcgccaccgt gctgacgacc ctgcagcagg gtgggttgga gccagtcgac gtcgagggcc
    10981 tgcgccgcca ctatgcacgc acgctgcagt gctggagcga ggccttcgag gcccgcgccg
    11041 aacacctgaa gaccctggtg gacgaaaaga cctggcgcgt ctggcgcatg tacctggtcg
    11101 gcagccagtg ggccttcgag cacgatgaga tctcgctgtt ccaggtgctg tgccggcgcg
    11161 ccgggcagcc ggctgccacc ctgccctggt cacgccgctg gatgtacaag gcggcgcact
    11221 gacggcgtgc gccagcctct ggcgagcccc gtgtgctcga aagcagcgcc ggtgacggcg
    11281 ggcctgtgcc gcgccacggg cggtgaccag gggccccctt ccggaccccg actcggcatc
    11341 accccccggc gtcgcgcgcc tgggcgttca ccgcgtcgtg ctgcgcgcgc accgccggcg
    11401 gccagcgcga cttgatccac gacagcacgg ccacgatctc cgcgtcgctc agcaccccgt
    11461 cataggccgg catggcgctg cggtagccgg gctggccgat gacccgggcc aggccgtgcc
    11521 tggtgatcgc cagcagttgc gcgtcggggt ggtgccaggt atggccgctg gcgtcgtgcg
    11581 gcggcgccgg ccgcaggccg tcgggacccg gttcgcgcca gcgcggctgg ccttcgccgt
    11641 gcacgccgtg gcagctggcg cagtgagcgg cgtacacgcg ctggcccagc gccagcacgg
    11701 ccgggtcgtc gggccgcagc aggctggccg ccgacccggg ccaccaggcg cgccaggcca
    11761 gcaacccgcc cgccagcagc atggcggccc cggcagccca ccacagcgcg ctgcgccgac
    11821 tcatgcaggc gctccagcga gcttgcccag gacgctcatc actatcattt atgtagcttc
    11881 cagcgcttgt ctggtaacga ttcagaccgt cagtctgatg tgaactggtt atcctggtcg
    11941 agtgtttcgc gctgtcaggc acttagagaa gccagaaaaa ggcttgcggc aaggcgcggc
    12001 tcgcagccca tgctggcggc cttggcaaca ggcgcaacgc cgccgccagc cgttttttgg
    12061 cttcccgcag ggcgagccgc caaggggcga tctgcttggt ggcgtggcct tgacatggct
    12121 ccaccatgcc tgcggccacg cgccgcgcag ctggcccctt ggcgactcgc tataagtacc
    12181 tgacaacgcg aaacactgca ctacgatggt ccgccgtgcc gccggaacac ctggctgctg
    12241 ccgtcgcgct gcaccagcag ggtgtcgtag ctgtcgcgcc ggccgccgta ggccgggccg
    12301 tccatgccgg gtgaacccac cggcatgccc ggcaccgcca gacccagcgc gcgcggcttg
    12361 tcgcgcagca ggcggtgcac gtcgtcggcg tgcacatggc cctcgaccac gtagccgccg
    12421 accagcgccg tgtggcagga gccatacctg gcggccagac ccaggcgcgc acgcacggcg
    12481 ttgttgccgg tgtcgtgcgc ggtcaccaca aagccgtgct gctgcatgtg ggcgatccag
    12541 tcgtggcagc agccgcagct ggcgtccttc cagacttcga cggcgatcgg ctgcgggcgc
    12601 cgctgcgcct gcacgcccgg caccagcgcc gcggctgccg ccagggccag caggcgcagt
    12661 gcctgacggc gacggggatg tgacttttgc atggcagacc tccggaacaa gggtggcgac
    12721 ggcgccgtcg ggcggcaggg gcgccgcaga ccgggcacac tggccagcag gcgccgattg
    12781 tcccgcaacc gtgcgccgcg ggccagccgc ccgccgatgc ccgtcatgac cgctcacccg
    12841 cgcgcttccc ccacccgcct ggccctgggc ctgagcgccg cctgcgccct gctgctgggc
    12901 ctgctggcgg cgatctgcct gctggcgctg cagctgcatt tcgagtcgcg cgaccgcaag
    12961 caactgcagt cgcacctgca caccacacag gcgctgctgg ccagcgtgga caacgccgcc
    13021 gcgctggcgg cgctgccggc ccggctggcc ggcgccttgc ccggcccgca gctgctggcg
    13081 gtgcgcgtgc agggcgccct gggccaaccg ctgtacgaac agggtgcgca caccgccatg
    13141 ccggccagcc tgctggcgca gcctggggtg gcgctgccga tcgcgctgca cagctggcag
    13201 cacgacggtc accactggcg cggcagcgcc ctgcgaatgc gcctgccgct ggacggcgcg
    13261 gccccgctga cggtggcgat ggcgctggac gttcagcccc aggcgcgctt tctcgccagc
    13321 ctgcgctggg ccctcgtcgg ctacgtgctg ctggcggcgc tgctgttcgg cctgctggcg
    13381 cgctggtggc tgggacggac cctagcgcgc ggacactgag cccgcgccac ggcgccgcgg
    13441 cgcgcgcccg ccttaagatg tctgcatgac caagctgccg ccacgctatc cacgccatcg
    13501 ccagtggtcc gacctgacca cgaccgactt cgccagcctc gatctggcgc gcgccatcgc
    13561 cgtgctgccg ctgggcgcca ccgaacagca cggcccgcac ctgccgctgt cggtggacgg
    13621 caacctggcg cacggcatgg tgcaggcggc cacgccgcac ctgccgatcg acctgccggc
    13681 gctgtttctg ccggtgcagc gcatcggcca cagccccgaa cacgcggcct ttgccggcac
    13741 gctgagcctg aaggccgaca ccgtgatccg cctgtggacc gacatcgccg agagcgtgca
    13801 cgccagcggg gtgcgcaagc tggtgctgtt caacgcccat ggtggcaacg tcgggctgat
    13861 ggacgtggtg gcgcgcgagc tgcgtgcgcg cctgtcgatg ctggtgtaca gcgtgagctg
    13921 gttcaacctg ccgctgcacg acgccgaggg caacgacctg ctggcgcgcg ttgcgcccga
    13981 ggagcagcgc ttcggcgtgc acggcggcca gctggaaacc gccatgatgc tgggcctgaa
    14041 gccgcgcctg gtgcgcaagc acaaggcgca ggacttcgcc tccagctcgc gcgagcgtgc
    14101 cgagggcttt gccatcctgg gcaatggccg cagcgccaag ctggcctggg cggcgcagga
    14161 cctgaacccg gccggtgtca ccggcaacgc cgccgccgcc acggtggaag acggcctggc
    14221 gctggtcgat gccgccgggc gcgccctggc cgcgctgctg caggagatcg accgcctgcc
    14281 ggccgacacg ctgc
//
LOCUS       DAOOOG010000049        14071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4681_length_14071_cov_23.156393, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000049 DAOOOG010000000
VERSION     DAOOOG010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14071
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4681_length_14071_cov_23.156393"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PK242_05560"
     CDS             <1..713
                     /locus_tag="PK242_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HOZ93517.1"
                     /translation="VTALEPDLLIIDEALAVGDQHFQKKCIERIQSFRDHGCTILFCS
                     HSPYHVRHLCDVALWLDHGRMMEFGSTEAVLGAYEKHSRLLGGERAETAAAPLPYERG
                     PDSALILSCDIADLGDGDPPTLHSPDLVATITVRGKGAERPNIGFMIEQERGVGITSL
                     ATHEEDAAPTEVEPGVWRSTLTFPDLPLHSGDYVISVFLFDETGLVTYDLWFQFKVIR
                     IVSPTLMPGLVRLPHHWS"
     gene            717..1643
                     /locus_tag="PK242_05565"
     CDS             717..1643
                     /locus_tag="PK242_05565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ93518.1"
                     /translation="MGLKELLDVTAAGPLPPEQARAALARGQALLAAGDRDGAATALR
                     QAALTRETQLAAHNLIESQGLPGAFAPWMGLDARIDPQDDVFGFFNGHGSWSSPLRDY
                     LADGWRTLSELMLLLERGGKPLAGFDAVLEFASGHGRFTRHLAKALGPGRVTVSDVVP
                     GAVAFSTTHFGVQGFVSAARPAQLHWPRRYGLVFVLSLFTHLPRATWSAWLARLWEAV
                     APGGVLVFTTHGALAAERAGVTLDAEGFHFHPESESHAIDVAEYGTSYTSRGFVEAEL
                     GLLRPAPTRVDFEPAWFWAHQDAWVLQRAEAA"
     gene            1640..2524
                     /locus_tag="PK242_05570"
     CDS             1640..2524
                     /locus_tag="PK242_05570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ93519.1"
                     /translation="MSTPVTPSEYDAWMRAHEAALDAGEPGAAAAIRAAREVPATRLA
                     AHNLIERRGLDGAFAQMFGLDCRIAPRDDIFHFFAGHPTSTNPLRDYLADGWRTLAEL
                     MQLLEAVDRPLLKLASVLEFASGHGRFTRHLVKAIGPQRVTVSDVVADAVGFARDTFG
                     VQGFVSDPQPEAVPWPRRYELVFVLSLFSHLPRHSWARWLASIWRAVAPGGLLVLSTH
                     GAEAARRAGVVLDDRGFFFAASSESSAIDAQEYGTSFTSEAFVREQVATLAGEAALIH
                     FAPVWFWNHQDAWVLRRA"
     gene            complement(2592..3503)
                     /locus_tag="PK242_05575"
     CDS             complement(2592..3503)
                     /locus_tag="PK242_05575"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ93520.1"
                     /translation="MDRVVAVLVTYHPDRQRLAQALQRLHAQVAACVVVDNTEGGPPA
                     DAAAAGDGEWIANGRNLGIGQAQNLGIARARALGASHVLLLDQDSLAPADLVPALLRT
                     LSQAAAAHAVAAVGPLCRDVKTGATLPLIRHSGWRVRRLRPGRSDGAVAVDYLPASGT
                     LIPLHALDRVGPLREDYFIDRVDVEWCLRARHQGWTILVDPAVELQHDLGRRALRLLG
                     RTVYVGQDWRACLHVRNSLAMALRAPIALRWRIDQWLKTPLYLLFYAAVAEGGRLRQA
                     RRLLRAAWDGVMGRLGPPPNARAPIKR"
     gene            complement(3514..7341)
                     /locus_tag="PK242_05580"
     CDS             complement(3514..7341)
                     /locus_tag="PK242_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ93521.1"
                     /translation="MAAPSPALHVYERDIDASERTSLSVLTRHIPAGARVLDLGCGSG
                     AIGRFLAARDGAGAGPIDGLTLSHDEARRAAPHYRRVEVADLESCDLPARFAPGSYDV
                     VVCADVLEHLRQPQRVMDQCRRLLAADGRALLSVPNAGYVGLVAELMAGEFRYRPEGL
                     LDDTHLRFFTRQSLRRFLADQGWTMDSLETVQRALPESEFRIAFDTLPPAVARHLLAL
                     PDALTYQFIIVSRPAPAQPLPAAAPIDTPAVAQAQFTAQLYWDDGQGMDEAHKLVTTG
                     VIGQRQQTLRFQLPAALPGLQGLKLDPADRPGILHLRSICLRSAAEPPRVAWQWSALA
                     DGTAALLASARTQQMVCGGALPGDASAAVLLTGDDPWIHLPIAADTLQQAAAAGPLLL
                     EVQLDWPMSADYLSLASVVGPLQAEQQRLREQHAADAGRLAELHQDHERLALENAHLR
                     HDRDALFAAGQSAQQQLAELHQQLHGMAEHVDNLRNLRAVRYTRLLAELVNGMRRPPG
                     RTPAPAGAAPAEADAGAPVPPPLSPTVDIIVPVYRGLRDTQVCIDAVLAGGPRTDWRL
                     IVINDCSPEPEVTAWLRDKAAAEPRITLLENEHNLGFVGTVNRGMRQSEHNDVLLLNS
                     DTEVAGDWLDRLRAAAYRQARAGTVTPFSGNATICSYPVFCADNPLPPGHSTASLDRL
                     FAQVNAGQSVPVPTGVGFCMYIRRDCLQAVGLFDEAHFGKGYGEENDFCQRAIGLGWH
                     NLHALDTYVLHTGGVSFGDSKSPREQAAMETLRRLHPGYDAQVQRFLQLDPARPARLA
                     VDWLRHTDGGRRQVVLVVQHQRGGGTERHILELAATLADTAVLVSLRPAGGQRVQLQL
                     IEQDAGRWAPSRRWAASFDLGHDGDALLRLLRDMAVSHVHYHHLLGHAPRVWTLAHEL
                     GVAYDVTCHDFYSFCTHITLTGRHGRYQMDAQGECCGGDHPPSLPETREPIDAWRIRN
                     RRFLQQARFVLTPSLDTAERMRRAMPGATLRCAPHTDILPGTLPMPQPPRLAAQEPLR
                     IVAIGALSVIKGADVLEETARLARRAGAPLEFHLIGYGYRHLQTAPGTALTVHGQYAD
                     EDLPALLQRLAPHLAWFPAQWPETYSYTLSAALQAGLPVAVPDLGAFAERVAERPWSW
                     VQAWDSSASQWLELFLRIRQQAFVDGQVQAAPPLSAPLQALRRQLGDWQYASDYLPAA
                     APAPWPEQLERARRVAAALAQAAPADAAAAPLGADRLYALALRLQRLPLLGRALRAVP
                     RSWRYRVRQLLSR"
     gene            7458..8570
                     /locus_tag="PK242_05585"
     CDS             7458..8570
                     /locus_tag="PK242_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ93522.1"
                     /translation="MLSLALLAFVLSLVATLWVRRLFQRWARGYDLDAPQRFHAGDTP
                     RMGGMGMLLGWTLALAALPLLQGIGMAGNIDGTRLHFSLWLLMLLPAFAGGVVEDLTQ
                     RLRVSSRMLLTLISGLLAWALLGVSVPQLGFAWVDGLWGAWPGLGMALAVLAVTGLPH
                     AFNIIDGYNGLAGSVALVVCLALAHVALQVGDRELAAVAVTLAAATAGFLVWNYPRGL
                     IFAGDAGAYLWGLLIAVLSLLLVQRHPIVSPWFPMLLLIYPVWETVFSIYRKLARGDS
                     PGMADALHLHQLVYRRLVRSVLHDDEAQSLLKRNNRTTPYLWGFMVLSVAPALLFWRY
                     SAVLLAFCVLFAVCYVWAYLSLIRFKVPRVLQPPRE"
     gene            8622..9515
                     /gene="argB"
                     /locus_tag="PK242_05590"
     CDS             8622..9515
                     /gene="argB"
                     /locus_tag="PK242_05590"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOZ93523.1"
                     /translation="MTTHTTALSPRDKAEILAQALPYIRQFHGKTMVIKYGGNAMTDP
                     ALQQDFAEDVVLLKLVGIHPVVVHGGGPQIEQALNRLGKKGEFIQGMRVTDAETMEVV
                     EWVLAGEVQQDIVGLIHQAGGKAVGLTGRDGGLIRARKLKLLDPKDPAIEHDVGQVGD
                     IVAIDPAVVRALQDDQFIPVVSPIGFGERNESYNINADVVAARLAMELQAEKLLMLTN
                     IPGVLDKAGQLLAELTPRQIDALVDDGTISGGMIPKISGALDAARSGVKAVHIIDGRV
                     PHALLLEVLGDQPFGTMIRAQ"
     gene            9653..10297
                     /gene="slmA"
                     /locus_tag="PK242_05595"
     CDS             9653..10297
                     /gene="slmA"
                     /locus_tag="PK242_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HOZ93524.1"
                     /translation="MPTAYDPSTDHSDHAGAAPAAGRKRPKPGERREQILQALAQMLQ
                     QPGAERVTTAALAARLGVSEAALYRHFASKAQMFEALIDFIESSVFGLIGQISERETD
                     GRARAARIVAMVLQFGERNPGMARVMAGDALVLEHERLQQRMNQWFDKLEAALRQSLR
                     EAAATPGTLTPDADAQVAAAVLTAFVAGRLQRFARSGFRRAPTEQGDACIARIL"
     gene            10373..11257
                     /locus_tag="PK242_05600"
     CDS             10373..11257
                     /locus_tag="PK242_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOZ93525.1"
                     /translation="MKLLRCGPPGRERPALLDADGVLRDLSALLPDIGPAQLSDTALA
                     RLARLRPQHLPVVRGRPRIGCPVAGIGKFIAIGLNYADHAAESGLPIPAEPVIFSKAI
                     SCIQGPNDDVMLPRGSRKSDWEVELGVVIGRTARQVSPREALRHVAGYCVVNDLSERA
                     FQLERGGTWDKGKGCDTFGPIGPWLVTRDEIAHPQRLGLWLELNGQRMQSGHTRTMIF
                     SVAQLISYVSRFMTLAPGDVLTTGTPPGVGMGRRDAAGRPAPRFLKAGDVLRLGIDGL
                     GEQCQRVVAFRPAPAAGR"
     gene            11368..11997
                     /locus_tag="PK242_05605"
     CDS             11368..11997
                     /locus_tag="PK242_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ93526.1"
                     /translation="MPAAETSVEPRPRPLPKGQQTKVAIVDAALRMAAQVGLEGLSIG
                     SLAETMGMSKSGVFAHFGSRDELQISVIREYYARFEAQVFQPAMAQPRGLPRVRALFE
                     NWMRFTSAELDSGCIFISGAVEFDDRPGPVRDALAEAVDAWIEAMTRAVAQACEQGHL
                     AADADPRQISFEIHALILALHYEARFMRRPGSMERAEQGFRNILARAGA"
     gene            12039..13835
                     /locus_tag="PK242_05610"
     CDS             12039..13835
                     /locus_tag="PK242_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ93527.1"
                     /translation="MPSYTPPLRDMQFVLHEVFHVADELKAMPTHAETDADTINAVLE
                     EAGKFAAGVAFPLNVSGDAEGCHLDAATHAVTTPKGFKEAYAAYVEGGWPALSCDPQY
                     GGQGLPHVVNQCLYEMLNSANQAWTMYPGLSHGAYEALHAHGTESQKATYLPKLVSGE
                     WTGTMCLTEPHCGTDLGLLRSKAEPQADGTYRITGNKIFISAGEHDMAANIVHLVLAR
                     LPDAPKGSKGISLFVVPKFLPKADGSLGERNAIYCTALEHKMGIHANATCQLSLDGAV
                     GTLVGEPHKGLAAMFVMMNAARLGVGNQSLGLTEVAFQNALVYAKDRIQMRSLSGTKA
                     KDKEADPIIVHPDVRKMLLTAKAYAEGARALQIFCTLLLDKAQHHPDERVRKECDELV
                     ALLTPIVKAFITDNGHISTNACMQVFGGHGFIKEWGMEQFVRDNRINMIYEGTNTVQS
                     LDLLGRKVLGNQGATLKKFGKLVGQLVAEEGVHEAMAEFINPIAMLGDQMTKFTTEIG
                     FRGMQNPDEVGAAAVDYLRVAGHLVFGYLFARMAQVSLRAIAAGSTDPFYTAKLQTAR
                     FYFAKLFPETATLMRTARAGSKVLMDTDAALA"
BASE COUNT         2021 a         4722 c         5187 g         2141 t
ORIGIN      
        1 tggtgacggc gctggagccc gacctgctga tcatcgacga ggcgctggcg gtgggtgacc
       61 agcactttca gaagaagtgc atcgagcgta tccagtcctt ccgcgaccat ggctgcacca
      121 tcctgttctg ctcgcacagc ccctaccacg tgcgccacct gtgcgatgtg gcgctgtggc
      181 tggaccatgg gcggatgatg gagttcggca gcaccgaggc ggtgctgggc gcctatgaaa
      241 agcattcccg cctgctgggc ggcgagcgcg ccgagaccgc cgcggcgccg ctgccttacg
      301 agcgcggccc cgattcggcc ctgatcctgt cgtgcgacat cgccgacctg ggcgacggcg
      361 atccgcccac gctgcacagc cccgatctgg tggccaccat cacggtgcgc ggcaagggtg
      421 ccgagcggcc caacatcggc ttcatgatcg agcaggagcg cggcgtgggc atcacctcgc
      481 tggccaccca cgaggaagac gccgcgccga ccgaggtcga gccgggggtg tggcgctcga
      541 cgctgacatt tcccgacctg ccgctgcaca gcggcgacta cgtgatcagc gtgttcctgt
      601 tcgacgagac cgggttggtg acctatgacc tgtggtttca gttcaaggtg attcggatcg
      661 tttcaccgac actgatgccg gggctggtgc ggctgcccca ccactggagt tgagcgatgg
      721 gcttgaagga attgctggat gtgaccgccg ccggcccgct gccgcccgag caggcgcgcg
      781 cggcgctggc gcgcggccag gcgctgctgg ccgccgggga tcgcgacggc gccgccaccg
      841 cgctgcgcca ggcggcgctg acgcgcgaga cgcagctggc ggcgcacaac ctgatcgaat
      901 cgcagggtct gccgggtgcg tttgcgccct ggatggggct ggatgcgcgc atcgacccgc
      961 aggacgacgt gttcggcttc ttcaatgggc acggcagctg gtccagcccg ctgcgcgact
     1021 acctggccga tggctggcgc acgctgtcgg agttgatgct gctgctcgag cgcggcggca
     1081 agcccctggc gggcttcgac gcggtgctgg agtttgccag tggccatggc cgcttcacgc
     1141 ggcacctggc caaggcgctg gggccggggc gcgtgacggt gtcggacgtg gtgccgggcg
     1201 cggtggcgtt ttcgaccacg catttcggcg tgcagggctt tgtgtcggcg gcgcggccgg
     1261 cgcagctgca ctggccgcgc cgctacggac tggtgttcgt gctgtcgctg ttcacgcatc
     1321 tgccgcgcgc cacctggagc gcctggctgg cgcggctgtg ggaggcggtg gcgccgggcg
     1381 gcgtgctggt gttcaccacg cacggggccc tggcggccga gcgtgccggc gtgacgctcg
     1441 atgccgaggg ctttcacttt caccccgaaa gcgagtcgca cgcgatcgac gtcgcggaat
     1501 acggcaccag ctacaccagc cgcggcttcg tcgaggccga gcttggcttg ctcaggccgg
     1561 cgccaacgcg ggtcgatttc gagccggcct ggttctgggc ccaccaggac gcctgggtgc
     1621 tgcagcgtgc ggaggccgca tgagcacgcc agtgacacca agcgaatacg acgcctggat
     1681 gcgggcgcac gaagcggcgc tggacgcggg cgagccgggc gcggcggcgg cgattcgcgc
     1741 cgcacgcgag gtgccggcca cgcggctggc ggcgcacaac ctgatcgagc ggcgtgggct
     1801 ggatggggcg tttgcccaga tgttcggtct ggactgccgc attgcgccac gcgacgacat
     1861 ctttcacttt tttgccggcc acccgacctc gaccaatccg ctgcgcgact acctggccga
     1921 cggctggcgc acgctggctg agctgatgca gcttctggaa gcggtggatc ggccgctgct
     1981 gaagctggca tcggtgctgg agtttgccag tggtcacggg cgcttcacgc gccacctggt
     2041 gaaggcgatc gggccgcagc gggtgacggt gagcgatgtg gtggccgacg cggtgggctt
     2101 tgcgcgcgac acgtttggcg tgcaaggctt cgtgtcggac ccgcagcccg aggcggtgcc
     2161 gtggccgcgc cgctacgagc tggtgttcgt gctgtcgctg ttcagccacc tgccgcgcca
     2221 cagctgggcg cgctggctgg ccagcatctg gcgggcggtg gcgccgggcg ggctgctggt
     2281 gctgtcgacc cacggcgcgg aggccgcgcg ccgcgccggc gtggtgctgg atgaccgtgg
     2341 gttctttttt gccgcctcca gcgagtccag cgccatcgat gcgcaggagt acggcaccag
     2401 cttcaccagc gaagccttcg tgcgcgaaca ggtggcgacg ctggcgggcg aggcggcgct
     2461 gatccatttt gcgcccgtgt ggttctggaa ccaccaggat gcctgggtgc tgcgacgggc
     2521 gtgagcgccg caccagaaag ctataaaaac aatagcttct tgcgcttgcc aggtgggcgc
     2581 cacaggtcgt tttatcgctt gatgggggcg cgtgcgttcg gtgggggacc caggcggccc
     2641 atcacaccgt cccacgccgc gcgcagcagg cggcgcgcct ggcgcagccg gccgccttcg
     2701 gccacggcgg catagaacag caggtacagc ggggtcttga gccactggtc gatgcgccag
     2761 cgcagcgcga tgggggcgcg cagcgccatg gcgagcgagt tgcgcacgtg caggcaggcc
     2821 cgccagtcct gccctacgta gaccgtgcgc cccagcagcc gtagcgcgcg ccggcccagg
     2881 tcgtgctgca gctcgaccgc ggggtcgacc aggatggtcc agccctggtg gcgggcgcgc
     2941 aggcaccatt cgacgtcgac gcggtcgatg aagtagtcct cgcgcagcgg gcccacgcgg
     3001 tccagcgcgt gcagcgggat cagggtgccg gaggcgggca ggtagtcgac cgccaccgcg
     3061 ccgtcgctgc ggccggggcg caggcggcgc acgcgccagc cgctgtggcg gatcagcggc
     3121 aacgtggcgc cggtcttgac gtcgcggcac agcggcccga cggcggccac cgcgtgggct
     3181 gcggcggcct ggctgagcgt gcgcagcagt gccggcacca gatcggctgg cgccaggctg
     3241 tcctggtcca gcagcagcac atggctggcg cccagtgcgc gcgcgcgggc gatgcccagg
     3301 ttctgcgcct ggccgatgcc caggttgcgg ccgttggcga tccattcgcc gtcgccggcc
     3361 gcggcggcgt ccgctggcgg gccgccttcg gtgttgtcga ccaccacgca ggccgccacc
     3421 tgagcgtgca ggcgttgcag cgcctgcgcc aggcgctggc ggtcggggtg gtaggtgacg
     3481 agcacggcca ccacgcggtc catgggcggc ccttcagcgg ctgagcagct ggcgcacgcg
     3541 gtagcgccag gagcgcggca cggcccgcag tgcccggccc agcagcggca ggcgctgcag
     3601 gcgcagggcc agggcgtaca ggcggtccgc gccgagtggc gctgccgccg cgtcggccgg
     3661 cgcggcctgg gccagcgccg cggccacgcg gcgggcgcgt tcgagctgct cgggccaggg
     3721 cgccggcgcc gccgccggca ggtagtcgct ggcgtactgc cagtcgccca gttgccggcg
     3781 cagcgcctgc agcggcgcgg acagcggcgg ggcggcctgc acctgcccgt cgacgaacgc
     3841 ctgctgccgg atgcgcagga acagttcgag ccactgcgag gcgctgctgt cccaggcctg
     3901 cacccaactc caggggcgct cggccacccg ttcggcaaag gcgccgaggt cgggcacggc
     3961 gaccggcagc ccggcctgca gggcggcgct gagggtgtag ctgtaggttt ccggccactg
     4021 cgccgggaac cacgccaggt gcggcgccag ccgctgcagc agggccggca ggtcttcgtc
     4081 ggcgtactgg ccgtgcacgg tgagggcagt gccgggggcg gtctgcaggt ggcgatagcc
     4141 gtagccgatc aggtgaaatt cgagcggtgc gccggcgcgc cgcgccaggc gtgcggtttc
     4201 ttccagcacg tcggcgccct tgatgacgct gagcgcgccg atggcgacga tgcgcagcgg
     4261 ctcctgggcc gccagtcgcg gcggctgggg catgggcagg gtgccgggca ggatgtcggt
     4321 gtggggcgcg cagcggaggg tggcgcccgg catggcgcgg cgcatgcgct cggcggtgtc
     4381 caggctgggc gtgagcacga accgcgcctg ctgcagaaag cggcggttgc ggatgcgcca
     4441 ggcgtcgatc ggctcgcggg tctcgggcag cgagggcggg tggtcaccgc cgcagcattc
     4501 gccctgcgcg tccatctggt agcggccgtg cctgccggtc agggtgatgt gggtgcagaa
     4561 gctgtagaag tcgtggcagg tgacgtcgta ggccacgccc agctcgtgcg ccagcgtcca
     4621 gacgcgcggc gcgtggccga gcaggtggtg gtagtgcacg tggctgaccg ccatgtcgcg
     4681 cagcaggcgc agcagggcgt ccccgtcgtg cccgagatcg aaactggcgg cccagcggcg
     4741 cgacggtgcc cagcggcccg cgtcctgctc gatcagctgc agctgcaccc gctgcccgcc
     4801 ggccgggcgc agcgacacca ggacggcggt gtcggccagc gtggcggcca gctccagaat
     4861 gtggcgctcg gtgccgccgc cacgctggtg ctgcaccacc agcaccacct ggcggcgccc
     4921 gccgtcggtg tggcgcagcc agtcgacggc caggcgcgct gggcgcgccg ggtcgagctg
     4981 caggaagcgc tggacctggg cgtcgtagcc ggggtgcagg cggcgcaggg tttccatggc
     5041 ggcttgctcg cgcgggcttt tgctgtcgcc gaagctgacg ccgccggtgt gcagcacgta
     5101 ggtgtcgagc gcgtgcaggt tgtgccagcc caggccgatg gcgcgctggc agaagtcgtt
     5161 ttcttcgccg tagcccttgc cgaaatgcgc ttcgtcgaac aggcccacgg cctgcaggca
     5221 gtcgcgccgg atgtacatgc agaagccgac gccggtgggc accggtacgc tctggccggc
     5281 gttgacctgg gcgaacagcc ggtccaggct ggccgtgctg tggccgggcg gcagcgggtt
     5341 gtcggcgcag aacaccgggt agctgcagat ggtggcgttg ccggagaacg gcgtcaccgt
     5401 gccggcccgc gcctgtcggt aggcggcggc gcgcaggcga tcgagccagt cgccggccac
     5461 ttcggtgtcg ctgttgagca gcagcacgtc gttgtgttcg gactggcgca tgccgcggtt
     5521 gacggtgccg acgaagccca ggttgtgctc gttttcgagc agcgtgatgc gcggctcggc
     5581 ggccgccttg tcgcgcagcc aggcggtgac ctcgggctcg gggctgcagt cgttgatgac
     5641 gatcaggcgc cagtcggtgc gcgggccgcc ggccagcacg gcgtcgatgc acacctgggt
     5701 gtcgcgcagg ccgcggtaga ccggcacgat gatgtccacc gtggggctga gtggcggcgg
     5761 cacgggggcg ccggcgtcgg cctcggccgg ggcggcgccg gcgggcgccg gggtgcggcc
     5821 gggcgggcga cgcatgccgt tgaccagctc ggccagcagg cgggtgtagc gcacggcgcg
     5881 caggttgcgc aggttgtcca cgtgctcggc catgccgtgc agttgctggt gcagctcggc
     5941 cagctgctgc tgcgcgctct ggccggcggc gaacagcgcg tcgcggtcgt gcctgaggtg
     6001 ggcgttttcc agcgccaggc gctcgtggtc ctggtggagt tcggcgaggc gcccggcgtc
     6061 ggcagcgtgc tgctcgcgca gccgctgctg ctcggcctgc agcgggccaa ccacgctggc
     6121 caggctcagg tagtcggccg acatcggcca gtcgagctgc acttccagca gcagcgggcc
     6181 ggccgccgcg gcctgctgca gggtgtcggc agcgatgggc aggtggatcc aggggtcgtc
     6241 accggtcagc agcacggctg cgctggcgtc gcccggcagc gcgccgccac acaccatctg
     6301 ctgcgtgcgg gccgaagcca gcagggcggc cgtgccatcg gccagggcgc tccactgcca
     6361 ggccacgcgc ggcggctcgg cggccgagcg caggcagatc gagcgcaggt gcaggatgcc
     6421 gggccggtcg gccggatcga gcttgagccc ctgcaggccc ggcagcgctg ccggcagctg
     6481 aaagcgcagg gtctgttggc gctggccgat gacgccggtg gtcaccagct tgtgcgcttc
     6541 atccatgccc tgaccgtcgt cccagtacag ctgggccgtg aactgcgcct gcgccacggc
     6601 aggggtgtcg atcggcgccg cggcgggcag cggctgggcc ggcgccggtc ggctgacgat
     6661 gatgaactgg taggtgagcg cgtccggcag ggccagcagg tggcgcgcca cggccggcgg
     6721 cagggtgtcg aaggcgatgc ggaattcgga ctccggcagc gcgcgctgca cggtttccag
     6781 gctgtccatg gtccagccct ggtcggccag aaagcgccgc agcgactggc gggtgaagaa
     6841 gcgcaggtgg gtgtcgtcca gcaggccctc gggccggtag cggaattcgc ccgccatcag
     6901 ctcggccacc aggcccacgt agccggcgtt ggggacggac agcagggcgc ggccgtcggc
     6961 ggccagcaac cggcggcatt ggtccatcac gcgctgcggc tggcgcaggt gctccagcac
     7021 gtcggcgcag acgacgacgt cgtagctgcc cggtgcgaac cgggcgggca ggtcgcagga
     7081 ttcgaggtcg gccacctcga cgcggcggta gtggggcgcg gcgcgccggg cttcgtcgtg
     7141 gctgagggtc aggccgtcga tcgggccggc gccggcgcca tcgcgcgcgg ccagaaagcg
     7201 cccgatggcg ccgctgccgc agcccagatc gagcacgcgg gcgccggcgg ggatgtggcg
     7261 ggtcagcacc gacagggagg tgcgctcgct ggcgtcgatg tcgcgttcgt agacgtgcag
     7321 cgcgggggag ggggcggcca tgcgatcggc agttgggtac cggctgttcg cgcgcgcgaa
     7381 ccgggacgac ggatgctacc atgcaaggct ttttgcgcac accgcgccgc aacgggccct
     7441 gaaccactcg tcactgcatg ctgtccctgg ccctgcttgc cttcgtgctg tctctggtgg
     7501 ccacgctgtg ggtgcgcaga ctgtttcagc gctgggccag gggttacgac ctcgacgcgc
     7561 cccagcgttt tcatgccggt gacacacccc gcatgggggg gatgggcatg ctgctgggct
     7621 ggacgctggc gctggcggcg ctgccgctgc tgcaagggat cggcatggcg ggcaacatcg
     7681 acggcacccg actgcacttc tcgctgtggc tgctgatgct gctgcccgcc tttgccggcg
     7741 gcgtggtgga agacctgacc cagcgcctgc gggtgagttc gcgcatgctg ctgaccctga
     7801 tttcgggcct gctggcctgg gcgctgctgg gggtgtcggt gccgcagctg ggtttcgcct
     7861 gggtcgatgg tctgtggggc gcctggccgg ggttgggcat ggcgctggcg gtgctggccg
     7921 tgaccgggct gccgcatgcc ttcaacatca tcgacggcta caacggcctg gccggttcgg
     7981 tggcgctggt ggtgtgcctg gcgctggcgc atgtggcgct gcaggtgggc gatcgcgagc
     8041 tggccgcggt ggccgtgacg ctggcggcgg cgacggcggg gtttctggtc tggaactacc
     8101 cgcgcggcct gatctttgcc ggcgatgccg gggcctacct gtggggcctg ctgatcgccg
     8161 tgctgagcct gctgctggtg cagcgccacc ccatcgtgtc gccctggttt ccgatgctgc
     8221 tgctgatcta cccggtgtgg gagacggtgt tctccattta ccgcaagctg gcgcgcggcg
     8281 actcgcccgg catggccgac gcgctgcacc tgcaccagct ggtgtaccgg cgcctggtgc
     8341 gcagcgtgct gcacgacgac gaagcgcaga gcctgctcaa gcgcaacaac cgcaccacgc
     8401 cgtacctgtg gggcttcatg gtgctgagcg tggcgccggc gctgctgttc tggcgctaca
     8461 gcgcggtgct gctggcgttc tgcgtgctgt tcgccgtgtg ctacgtgtgg gcctacctgt
     8521 cgctgatccg cttcaaggtg ccgcgcgtgc tgcagccgcc gcgcgagtga caaccctatg
     8581 atcgagcttt tgcaccttgc tgccgtttcg cgccacctgc catgaccacc cacaccaccg
     8641 ccctttctcc gcgtgacaag gccgaaatcc tggcccaggc gctgccctac atccgccagt
     8701 ttcatggcaa gaccatggtc atcaagtacg gcggcaacgc catgaccgac ccggcgttgc
     8761 agcaggactt tgccgaggac gtggtgctgc tcaagctggt gggcatccac ccggtggtgg
     8821 tgcacggcgg cgggccgcag atcgagcagg ccctgaaccg cctgggcaag aagggcgagt
     8881 tcatccaggg catgcgcgtg accgacgccg aaaccatgga ggtggtcgag tgggtgctgg
     8941 ccggtgaggt gcagcaggac atcgtgggcc tgatccacca ggccggcggc aaggcggtgg
     9001 gcctgacggg gcgcgacggc ggcctgattc gggcgcgcaa gctcaagctg ctcgacccca
     9061 aggacccggc catcgagcac gacgtgggcc aggtgggcga catcgtggcg atcgacccgg
     9121 cggtggtgcg cgccctgcag gacgaccagt tcatcccggt ggtcagcccg atcggctttg
     9181 gcgaacgcaa cgagagctac aacatcaacg ccgacgtggt ggcggcgcgc ctggccatgg
     9241 agctgcaggc cgaaaagctg ctgatgctga ccaacatccc gggcgtgctg gacaaggccg
     9301 gccagctgct ggcggagctg acgccgcgcc agatcgatgc cctggtggac gacggcacca
     9361 tctccggcgg catgatcccc aagatcagcg gcgcactgga cgccgccagg agcggcgtga
     9421 aggcggtgca catcatcgac ggccgggtgc cgcacgcgct gctgctggag gtgctgggcg
     9481 accaaccctt cggcaccatg atccgcgccc agtgagccgc agccgccttc gggcggctcc
     9541 ggtggcgagc gctcacttgg cggctctgtg gcgcggagca aacgccggcg caatcaggct
     9601 tggaccgcgc aaggccttgt gcgagaatca tttggaaaca aatgatgccg ccatgcccac
     9661 cgcctacgat ccttcgaccg accactccga ccacgccggg gcggcccctg ccgccgggcg
     9721 caagcgcccc aagccaggcg agcggcgcga gcagatcctg caggcgctgg cgcagatgct
     9781 gcagcagccc ggcgccgagc gcgtgaccac cgccgccctg gcggcccgcc tgggggtgag
     9841 cgaggcggcg ctgtaccgcc acttcgccag caaggcgcag atgttcgaag cgctgatcga
     9901 cttcatcgaa agcagcgtgt tcggcctgat cggccagatc agcgagcgcg agaccgacgg
     9961 ccgagcgcgc gcggcccgca tcgtggcgat ggtgctgcag ttcggcgaac gcaaccccgg
    10021 catggcgcgc gtgatggcgg gcgacgcgct ggtgctggag cacgaacgcc tgcagcagcg
    10081 catgaaccag tggttcgaca agctggaggc ggcgctgcgc cagagcctgc gcgaggcggc
    10141 ggcgaccccc ggcacgctga cgccggacgc cgacgcgcag gtcgcggcgg cggtgctgac
    10201 cgccttcgtg gcggggcgac tgcagcgctt tgcgcgttcc ggctttcggc gtgcgccgac
    10261 cgagcagggc gacgcctgca tcgccagaat actataaaaa cagtagctgc tggcgcttgt
    10321 ctgacaagcg ccatgacctg attttgttct tatcgatcct tcggggtacc tgatgaagct
    10381 gctgcgctgc ggcccgccgg gccgcgaaag accggccctg ctggacgccg acggcgtgct
    10441 gcgcgatctg tcggcgctgc tgcccgacat cggcccggcg cagctgtcgg acaccgcgct
    10501 ggcgcgcctg gcgcggctgc gcccgcagca cctgccggtg gtgcgcggcc ggccacgcat
    10561 cggctgtccg gtggcgggca tcggcaagtt catcgccatc ggcctgaact acgccgacca
    10621 cgcggccgag tctggcctgc cgatcccggc cgagccggtg atcttcagca aggcgatctc
    10681 ctgcattcag ggtccgaacg acgacgtcat gctgccgcgc gggtcccgca agagcgactg
    10741 ggaggtcgag ctcggcgtgg tgatcggtcg cacggcgcgc caggtgtcgc cgcgcgaggc
    10801 gctgcgccat gtggcgggct actgcgtggt caacgacctc agcgagcgcg cctttcagct
    10861 cgagcgcggg ggcacctggg acaagggcaa gggctgcgac accttcggcc ccatcgggcc
    10921 gtggctggtg acgcgcgacg aaatcgccca cccgcagcgc ctggggctgt ggctggagct
    10981 gaacggtcag cgcatgcaaa gcggtcacac gcgcaccatg atcttcagcg tggcgcagct
    11041 gatcagctac gtcagccgct tcatgaccct ggcgccgggc gacgtgttga ccaccggcac
    11101 accgccgggc gtgggcatgg ggcggcgcga cgccgccggc cgcccggcgc cgcgctttct
    11161 gaaagctggt gacgtcttgc ggctgggtat cgacggcctg ggtgagcagt gccagcgcgt
    11221 ggtggcgttt cgccccgcgc ccgccgcagg acgctgaatt cctaagggaa aacccggacc
    11281 attgcccagg ccccacggct aggcaggccg gatttccaga tataaaatag aacgaccgtt
    11341 cgttttattc tggatatgtc ccgttccatg cctgccgctg agacctcggt cgagccgcgc
    11401 ccgcgtccgc tgcccaaggg ccagcagacc aaggtggcca tcgtcgatgc cgccctgcgc
    11461 atggccgccc aggtggggct ggaggggctg tccatcggct cgctggccga gaccatgggc
    11521 atgagcaagt cgggcgtgtt cgcgcatttc ggttcgcgcg acgagctgca gatttccgtg
    11581 atccgcgaat actacgcccg cttcgaggcc caggtgttcc agcccgccat ggcgcagccg
    11641 cgcggtctgc cgcgggtgcg tgcgctgttc gagaactgga tgcgctttac cagcgccgaa
    11701 ctcgattcag gctgcatctt catcagcggc gcggtcgagt tcgatgaccg tcccggcccg
    11761 gtgcgcgatg ccctggccga ggcggtcgac gcctggatcg aggccatgac ccgcgccgtg
    11821 gcccaggcct gcgagcaagg ccacctggcc gccgacgccg acccgcgcca gatcagcttc
    11881 gagatccacg cactgatcct ggccctgcac tacgaagcac gcttcatgcg ccgccccggc
    11941 tccatggagc gcgccgagca gggctttcgc aacatcctcg cgcgcgccgg cgcctgaggt
    12001 tctctcaccc accatcgtcc atcaggagaa acgccgccat gcccagctac accccgcccc
    12061 tgcgcgacat gcagttcgtg ctgcatgaag tcttccacgt cgccgacgaa ctgaaggcca
    12121 tgcccacgca tgccgagacc gacgccgaca ccatcaacgc cgtgctggaa gaggccggca
    12181 agttcgcagc cggggtggcg tttccgctca acgtcagcgg cgacgccgag ggctgccacc
    12241 tcgacgccgc cacgcacgcc gtgaccacgc ccaagggctt caaggaggcc tacgccgcct
    12301 acgtcgaagg cggctggccg gcgctgtcgt gcgacccgca gtacggtggc cagggcctgc
    12361 cgcacgtggt caaccagtgc ctgtacgaga tgctcaacag cgccaaccag gcctggacca
    12421 tgtaccccgg cctgtcgcac ggcgcctacg aagccctgca tgcccacggc accgagagcc
    12481 agaaagccac ctacctgccc aagctggtga gcggcgaatg gaccggcacc atgtgcctga
    12541 ccgagccgca ctgcggcacc gacctgggct tgctgcgcag caaggccgaa ccgcaggccg
    12601 acggcaccta caggatcacc ggcaacaaga tcttcatcag cgccggcgag cacgacatgg
    12661 ccgccaacat cgtgcacctg gtgctggccc gcctgcccga cgcgcccaag ggcagcaagg
    12721 gcatcagcct gttcgtggtg cccaagttcc tgcccaaggc cgacggcagc ttgggcgagc
    12781 gcaacgccat ctactgcacg gcgctggaac acaagatggg cattcacgcc aacgccacct
    12841 gccagctgtc gctggacggc gccgtgggta ccctggtggg cgagccccac aagggcctgg
    12901 ccgccatgtt cgtgatgatg aacgccgccc gcctgggcgt gggcaaccag tcgctgggcc
    12961 tgaccgaggt ggcgttccag aacgcgctgg tctatgccaa ggaccgcatc cagatgcgct
    13021 ctttgagcgg caccaaggcc aaggacaagg aggccgaccc catcatcgtc caccccgacg
    13081 tgcgcaagat gctgcttacc gccaaggcct atgccgaggg cgcgcgcgcg ctgcagattt
    13141 tctgcacact gctgctggac aaggcgcaac accacccgga cgagagagtg cgcaaggagt
    13201 gtgacgaact ggtggcgctg ctcaccccca tcgtcaaggc cttcatcacc gacaacggcc
    13261 acatcagcac caacgcctgc atgcaggtgt tcggcggcca tggcttcatc aaggaatggg
    13321 gcatggagca gttcgtgcgc gacaaccgca tcaacatgat ctacgagggc acgaacaccg
    13381 tgcagtcgct cgacctgctg ggccgcaagg tgctgggcaa ccagggcgcg acgctgaaaa
    13441 aattcggcaa gctcgtgggc cagctggtgg ccgaggaagg cgttcatgaa gcgatggccg
    13501 agttcatcaa cccgattgcc atgctgggcg accagatgac caagttcacc accgagatcg
    13561 gcttccgtgg catgcagaac cccgacgagg tgggcgccgc cgccgtggac tatctgcgcg
    13621 tggccggcca tctggtgttc ggctacctgt ttgcgcgcat ggcccaggtg tcgctgcgcg
    13681 cgattgccgc gggcagcacc gaccccttct acaccgccaa gctgcagacg gcgcgcttct
    13741 acttcgcgaa gctgttccct gaaaccgcga cgctgatgcg cacggcacgc gcgggcagca
    13801 aggtgctgat ggacacggac gccgcgctgg cctgatgtct gccaactccc tcccccttgg
    13861 gggagggcag gggtgggggc cggcgacgtt ggagtcgtga tgccggcgct ccaggccccc
    13921 atccccgcct tcccccagag cgggaaggag cgaagttcaa gcagtttgtg ccaaggagcc
    13981 cttatgagac aagcgctagc agctatcgtt ttgattgcaa tcgccggccc cgcactggcc
    14041 cagatgagcc cgctgggcct gtggcgcagc a
//
LOCUS       DAOOOG010000050        14022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4710_length_14022_cov_24.993198, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000050 DAOOOG010000000
VERSION     DAOOOG010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14022
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4710_length_14022_cov_24.993198"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PK242_05615"
     CDS             complement(<1..394)
                     /locus_tag="PK242_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOZ93528.1"
                     /translation="MLLAGSLSTAALAQTPASAPVAGTLGTVTVRDSATTDGAATTSK
                     SQLRATRTEIGKGNQELRDIPQSVTVMTERLIQDRNLDDFRDVLRATAGVTFQAGETG
                     EEDVRLRGFSLGQAGDIYRDGMRDAPLYE"
     gene            complement(727..1203)
                     /gene="bfr"
                     /locus_tag="PK242_05620"
     CDS             complement(727..1203)
                     /gene="bfr"
                     /locus_tag="PK242_05620"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOZ93529.1"
                     /translation="MQGDAKVIAHLQAQLKNELTAINQYFLHHRMLKHWGLERLARKE
                     YEESIGEMKHADRLMDRILMLDGLPNLQDLGKLMVGENVPEILRCDLKLEQAAQATIK
                     DGIAHCESVRDYVSRDLLQDILDDTEEHIDFLETQIELLDKVGLQNYLQSQMGAPS"
     gene            1382..3190
                     /locus_tag="PK242_05625"
     CDS             1382..3190
                     /locus_tag="PK242_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOZ93530.1"
                     /translation="MTDTRPAAQPQPEPQRRRARDRLKGRQPEAGARDEVRALIGTPG
                     PDGHRRDLLIEHLHRLNDRYHGLFERHLVALAAEMRLSMAEVYEVASFYHHFEVRKDG
                     EAAPRLTVRVCTSLSCQLAGADALLARTRELLGAEVQVLAAPCIGRCEQAPAALVGPH
                     GVGQASAERLVQASNRALALDGPAPTAIKPIVFDAYTQAGGYTLAQAVARGEPGHEAI
                     IAALEHAGLRGLGGAGFPAGRKWRIVRDLARSAERRAAPSEAAAPSGGSGPRAAGERG
                     GMTYLAINIDEGEPGTFKDRWYLERDPHRFLEGLLIAAQVVGVQGVYIYLRDEYPECR
                     TILTQALADLRAAPGLRELLPPTELRRGAGAYICGEESAMLESIEGKRGQPRLRPPYI
                     AQVGLFGRPTLEHNFETLYWLRDILQRGPDWFAAQGRHGRQGMRSFSVSGRVRQPGVK
                     WAPAGITLRELVDEYCGGMLDGHELYAFLPGGASGGIFPARLADVPLDFDTLQPHGGF
                     IGSAGVIVLSQHDKARDAALNMMRFFAAESCGQCTPCRVGTAKAARLMEAPVWDAATL
                     TDLSQVMQDASICGLGQAAPNPMQCVQRYFAHEVEA"
     gene            3187..6033
                     /gene="fdhF"
                     /locus_tag="PK242_05630"
     CDS             3187..6033
                     /gene="fdhF"
                     /locus_tag="PK242_05630"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOZ93531.1"
                     /translation="MTPALACIAFELDGQPATAFEGETILKAAERHGVQIPRLCFTDG
                     LRADGNCRACVVEVDGERTLAPSCCRAVQPGMKVRAASERARRAQDMVLELLLAELPA
                     AGFKWLDDDATQPHGELSQWASQRKVGVRQQLQNLQRPRPTPDLSHPAMAVNLDACIQ
                     CTRCVRACREAQVNDVIGYAGRGAEARIVFDLDDPMGASSCVACGECVQACPTGALMP
                     KTHIGPQRVDRSVDSVCPFCGVGCQITYHVRDEKIVSVSGRNGPANAGRLCVKGRFGF
                     DYAASPDRLTRPLIRIAGVPKDPAHCQQPPDWRTVFREASWDEALDAAAGGLRALKSR
                     HGPRALAGFGSAKGTNEEAYLFQKLVRTGFGSNNVDHCTRLCHASSVAALLEGVGSGA
                     VSNPVRDVEHAELILVIGANPTANHPVAASWMKNAARRGTRIVLADPRATDIARHCWR
                     VLQFRPGSDVALLNALLHVIVTEGLCNESFLRQRADHFAALAANVQGYSPEAMAPICG
                     IPAETLREVARAYATARSAMILWGMGVSQHVHGTDNARCLIALATCTGQLGRPGTGLH
                     PLRGQNNVQGASDAGLIPMMYPNYQRVDSPDARTWFEDFWGQPLDPEPGYTVVEIMRQ
                     ALAADDDPHKVRGMYIMGENPAMSDPDLNHTRHALASLSHLVVQDIFLTETAWLADVV
                     LPASAWPEKTGSVSNTDRMVQLGRQALAPPGEARADLWIIQQLAQRLGLPWNYENDSC
                     SRLPDKRQGPFFLEEPHGVAAVYEEMRRAMHGAIAGIGWDRLVRESSVTYPCLSEDDP
                     GQPIVFTERVPTPNGRVQLVPADIIAPDERPDADYPMVLITGRQLEHWHTGAMTRRAV
                     VLDALEPAPTVSMHGDDLARLGLVAGELVRVSSRRGRVCLTARRDDGTPVGGVFIPFA
                     FREAAANLLTNAALDPFGKIPEFKYCAVRVEPLA"
     gene            6030..6860
                     /locus_tag="PK242_05635"
     CDS             6030..6860
                     /locus_tag="PK242_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOZ93532.1"
                     /translation="MTVHLTRASAPLTERVLAVDELGQAVEVSIPAERALTLYVDKRE
                     LVTLMTLGAHPEWLVLGYLRNQRLLAGPAELASTTVDWDAGAAAVRTRHGIADLEMRT
                     RRRTVTSGCGQGSVFGDLMADLEALDLGAGRLALDDLYGLLNAIRLQESTYKSAGSVH
                     GCALFEGQRMLLFVEDVGRHNAIDTIAGWMWLQGDGGGLRGDDKVFYTTGRLTSEMVI
                     KAAQMGVPIVVSRSGITQMGLQLARRLGLCAVGRALNRRFLCYSAPERLGLPVPPHGA
                     "
     gene            complement(6867..7763)
                     /locus_tag="PK242_05640"
     CDS             complement(6867..7763)
                     /locus_tag="PK242_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOZ93533.1"
                     /translation="MPIFSTLRIDKDPAQPRIARLLLNRPERLNAINDDMPGEIRAAV
                     DWAVADDEVHVIVVEGAGKGFCGGYDLAATAEQHIEHPCQQESAPWDPALDYRAMKRF
                     TEDFMSLWRCPKPTIAKVHGAAVAGGSDIALCCDLLVMAEDARIGYMPTRVWGCPTTA
                     MWTYRLGPTRAKQLMFTGDVIDGRTAADWGLANQAVPAAELEAATLRLAARIAGVPRG
                     HLQMHKLVVNQVMLTMGLEQTQMLATVFDGITRHNPEGVWFRRHAQQHGFKSAVEWRD
                     SGRPIPEGAEAQALIRAMQGQG"
     gene            7841..10363
                     /locus_tag="PK242_05645"
     CDS             7841..10363
                     /locus_tag="PK242_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOZ93534.1"
                     /translation="MNLSSWTLGWRTLWRDLRAGELRLLMVAVTLAVAALSSVGFFAD
                     RLQGGLQRDARQLLGGDVVLVSDHAPAPGWTQRAAAEGLDSVLTLGFPTMGRAPEAQG
                     GATRLVALKAVERGYPLRGRLRLAQHAQDRAGTPTDAVPASGQVWVDPSLLEALALRR
                     GDTLLLGDSALRITELIALEPDRGTGFMSFAPRVLMNAADLPATGLVQPASRITWRLA
                     LAGPEPAVQRYRAWAETELARPGMAGLRLETLDTGRPEMRTTLDRAAKFLNLVALLAA
                     LLSAVAVALAARAFAARHLDDCAMLRVLGLPQRRIAGAYVTEFGLVGLSASLAGVLIG
                     WLVHHIFVALLAGLVDSALPAPGPWPALLGLGIGMTLLVAFGLPPVLQLAQVPPLRVL
                     RRDVGALRPASLAVLGLGVAGFAALLLAVSADLTLGLIAVGGFAVAVALFALLSWVAV
                     RLLRRWVHEQRAPRWLVLATRQLSARPAYAVVQVSSLAVGLLALVLLVLLRTDLIASW
                     RAATPADAPNRFVINIMPEQGEAFRAVLRQAGVTRMDWYPMFRGRLVAINGQPVNPAD
                     YPEERARHLVDREFNLSHAAERPAHNDIAAGHWTTDEAGAISVEQGLAETLRLRLGDR
                     LRFDVAGMPLEARITSLRKVDWASMHVNFFAMFPRADMPDVPVTWIAAYRAPGQPGFD
                     HALVRQFPNITHVDMEQSIAQVQRVLDQVVRAVEFLFGFTLAAGLIVLFAAVSATREE
                     RAREFAVMRAVGAGSALLARVQQAELVGVGLLAGVLASAVAMGIGWAMARHAFDFHWT
                     ASPWVPLLGGVAGAGLALAAGWWGLREVLQRPVVQTLRRVAE"
     gene            complement(10484..10789)
                     /locus_tag="PK242_05650"
     CDS             complement(10484..10789)
                     /locus_tag="PK242_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93535.1"
                     /translation="MSARFFPLASTLAWTLIAGAASAAMPASGEGPFFNDPIRGPSDL
                     TRAEVMAMAIQSPPASGEGNGSQTTAPTPQPVDRETVVAELRDAMAQGFRPATGDIG"
     gene            10982..11896
                     /locus_tag="PK242_05655"
     CDS             10982..11896
                     /locus_tag="PK242_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ93536.1"
                     /translation="MLLRTFREVALRHNFSTAARTLDLAPATVSKYVAELEARFHLRL
                     FHRSTRRVTLTDAGQLLLDHCAQVIDLIDRTHDAMNERAQRPSGRLNLTAPHGLTRTI
                     LVRRLADFMLQHSDVSINLNLTNRMVDLVEDGVDVAFRVGAVADNSLIVRRLLPLEMV
                     VAATPAYWRAHGRPEHPRELATHRTLAMAPTGETPHWQFSIGGKLFDLPLHPVFCASD
                     SSPLVPLALQGVGVLRGSRMLLGEWIDDGRLEPLFAAYSPRNLWLYAAYAQRRHPSAA
                     LRALLDFLEQQMRPTVPSVPHAVSPRLH"
     gene            12018..12641
                     /locus_tag="PK242_05660"
     CDS             12018..12641
                     /locus_tag="PK242_05660"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOZ93537.1"
                     /translation="MAQLFTVHPDNPQPRLLKQAAALLHGGGVLAVPTDSSYALVCHL
                     DDKAAVDRLRRIRQVDERHHLTLLCRDLSELASYARVDNAQFRRLKSGTPGPFTFILE
                     ASKEVPRRVSHPQRKTIGLRVPDHPALRELLALHGAPLLATTLILPGEGEPLNDAGAI
                     LEQLGHQIAGVIDAGACPLAPTTVVDLSGDAPEVLRRGRGDPARLGL"
     gene            12678..13346
                     /locus_tag="PK242_05665"
     CDS             12678..13346
                     /locus_tag="PK242_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOZ93538.1"
                     /translation="MDFANIIQTLALYALPVLFAITVHEAAHGYAARHFGDGTAAFLG
                     RVTLNPARHIDPIGTIAMPLFLYIATGGAFVFGYAKPVPVNFGQLRHPKRDMVWVALA
                     GPASNLAMALAWGALLIVLDGVGVTEPFFLKMCQAGVLVNVVIGVFNLFPLPPLDGGR
                     ILVGLLPRGPALALSRIEPWGFFIVMALVVAGVISNLWMRPLMALSFHLLDSILAPLQ
                     ALFG"
     gene            13351..>14022
                     /gene="trpS"
                     /locus_tag="PK242_05670"
     CDS             13351..>14022
                     /gene="trpS"
                     /locus_tag="PK242_05670"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOZ93539.1"
                     /translation="MASSVSRRVLTGITTTGTPHLGNYVGSIRAAVRASREPGVQSFY
                     FLADYHALIKCDDPARIQRSTLEIAASWLAAGLDPEQVTFYRQSDIPEIPELTWLLTC
                     VTGKGLLNRAHAYKAMLDKNAATGADPDSDVTAGLFMYPVLMAADILMFKAHQVPVGR
                     DQIQHIEMARDIAASFNHLYGELLTLPEALIDDNVALLPGLDGRKMSKSYDNTIALFG
                     PREQLR"
BASE COUNT         1966 a         4846 c         4974 g         2236 t
ORIGIN      
        1 gctcgtacag cggcgcgtcg cgcatgccgt cgcggtagat gtcgccggcc tggccgagcg
       61 agaagccgcg caggcgcacg tcttcctcgc cggtttcgcc ggcctggaag gtgacgccgg
      121 cggtggcgcg cagcacgtcg cggaaatcgt ccaggttgcg gtcctggatc agccgctcgg
      181 tcatcaccgt gacgctttgc ggaatgtcgc gcagctcctg gttgcccttg ccgatctcgg
      241 tgcgggtggc gcgcagctgt gacttgctgg tggtggcggc gccatcggtg gtggcgctgt
      301 cgcgcacggt gacggtgccc agggtgccgg ccaccggggc gctggcgggc gtctgggcca
      361 gcgcggcggt gctcagcgag ccggccagca gcatcgctcc caggggcagc agcggggtgc
      421 tgtctgcttc aaaaacagga gcattcggcg cttgctggat cagagattct ggcttttttg
      481 ctttggattt cgaacggatg ggcggcatgg ggtactcagc agaaaacgaa aacggagaag
      541 tgctggctac ctgggccggg gcggaaagac gggagggtgg ctttccgagg ccgttcgctg
      601 gcttgcaatg aagctgttga tcttaagcaa aatgataatc attgtcattc aatttgttgc
      661 agatcaacga cgggcacgaa aaaggggcct tgcggcccct gtctgtgcgg tcggtggcgg
      721 cgcgcctcag cttggcgcac ccatctggct ttgcaggtag ttctgcaggc cgaccttgtc
      781 cagcagctcg atctgggttt ccagaaagtc gatgtgctct tcggtgtcgt ccaggatgtc
      841 ctgcagcaga tcgcgcgaga cgtagtcgcg cacgctctcg caatgggcga tgccgtcctt
      901 gatggtggcc tgtgccgctt gctcgagctt gaggtcgcag cgcaggattt cgggcacgtt
      961 ctcgcccacc atcagcttgc ccaggtcctg caagttgggc aggccatcga gcatcaggat
     1021 gcggtccatc aggcggtcgg cgtgcttcat ttcgccgatg gattcttcat attccttcct
     1081 ggccaggcgc tccaggcccc agtgcttgag catgcggtgg tgcaggaagt actggttgat
     1141 ggcggtcagc tcgttcttca gctgagcctg caggtgggca atgactttgg cgtcgccttg
     1201 catggcgttc tccttgtggg tggcgatggc ccagccattc taggagcgcg ttcacctgcg
     1261 ttcagtgcag ggattttcgg aatgagaccg gttcgcgtgc ggagcgcgac cgcagggcgt
     1321 tgggctcggg ctttcctcgg gctttcgcca tgcgacgcgg cgctgcacaa tgcccgcagc
     1381 catgaccgac acccgccctg ccgcccagcc ccagcctgaa cctcagcgtc ggcgtgcgcg
     1441 cgaccggctc aagggccgcc agcccgaggc cggtgcgcgc gacgaggtgc gcgccctgat
     1501 cggcacgccc ggccccgacg gccaccggcg cgacctgctg atcgagcacc tgcaccggct
     1561 gaacgatcgc taccacggcc tgttcgagcg ccacctggtg gcgctggcgg ccgaaatgcg
     1621 cctgtcgatg gccgaggtgt acgaggtcgc cagcttctac catcacttcg aagtgcgcaa
     1681 ggatggcgag gccgcaccac gtctgacggt gcgggtgtgc acatcgctga gctgccagct
     1741 ggccggtgcc gatgccttgc tggcgcgcac gcgcgagctg ctgggcgccg aggtgcaggt
     1801 gctggccgcc ccctgcatcg gccgctgcga gcaggcgccg gccgcgctgg tgggaccgca
     1861 tggcgtgggg caggccagcg ccgagcggct ggttcaggca tcaaatcggg ctctggcgct
     1921 tgatggtcct gcgccaacag ctataaaacc aatagtgttt gacgcctaca cgcaagctgg
     1981 cggctacacc ctggcgcagg ccgtggcgcg cggcgagccg ggccacgagg ccatcatcgc
     2041 cgcgctcgag cacgccggcc tgcgcggcct gggcggcgcc ggctttcccg ccgggcgcaa
     2101 gtggcgcatc gtgcgcgact tggcccgctc cgccgagcgc cgggccgccc caagcgaggc
     2161 ggcggccccc tcggggggca gcggaccacg cgcagcgggc gagcgtgggg gcatgacata
     2221 tctggcgatc aacattgatg aaggcgagcc cggcaccttc aaggaccgct ggtacctgga
     2281 gcgcgacccg caccgctttc tggaaggtct gctgatcgcc gcgcaggtgg tgggggtgca
     2341 gggcgtgtac atctacctgc gcgacgaata ccccgaatgc cgcaccatcc tgacgcaggc
     2401 gctggccgac ctgcgcgctg cgcccgggct gcgcgagctg ctgccgccga ccgagctgcg
     2461 gcgcggcgcg ggcgcctata tctgcggcga agaatcggcg atgctggaga gcatcgaggg
     2521 caagcgcggc cagccgcgcc tgcgcccgcc ctacatcgcc caggtggggc tgtttggccg
     2581 gcccacgctg gagcacaact tcgagaccct gtactggctg cgcgacatcc tgcagcgcgg
     2641 ccccgactgg tttgccgcgc aggggcgcca cggccgccag ggcatgcgtt cgttcagcgt
     2701 cagcgggcgg gtgcggcagc ccggcgtcaa gtgggcgccg gccggcatca cgctgcgcga
     2761 gctggtggac gaatactgcg gcggcatgct ggacgggcat gagctgtacg ccttcctgcc
     2821 cggcggcgcc tcgggcggca tctttccggc ccggctggcc gacgtgccgc tggacttcga
     2881 cacgctgcag ccgcacggcg gcttcatcgg cagcgcgggc gtgatcgtgc tcagccagca
     2941 cgacaaggcg cgtgacgcgg cgctgaacat gatgcggttt tttgccgccg agagctgcgg
     3001 ccagtgcacg ccctgccggg tgggcactgc caaggcggcc cgcttgatgg aagcgccggt
     3061 gtgggacgcg gccaccctga ctgatctgag ccaggtgatg caggacgcca gcatctgcgg
     3121 cctgggccag gccgcgccca accccatgca gtgcgtgcaa cgctactttg cgcacgaggt
     3181 ggaggcatga cgccggcact cgcttgcatt gctttcgaac tcgacggcca gcccgccacc
     3241 gccttcgagg gcgaaaccat cctgaaggcc gccgagcgcc acggcgtgca aatcccgcgc
     3301 ctgtgcttca cggacggcct gcgcgccgac ggcaactgcc gcgcctgcgt ggtcgaggtg
     3361 gacggcgagc gcacgctggc gcccagttgc tgccgcgccg tgcagccggg catgaaggtg
     3421 cgcgccgcca gcgagcgagc gcgccgcgcc caggatatgg tgctggagct gctgctggcc
     3481 gagctgcccg cggccggttt caagtggctg gacgatgacg ccacgcagcc ccatggcgaa
     3541 ctgagccagt gggccagcca gcgcaaggtg ggtgtgcgcc agcagctaca aaatttgcag
     3601 cgcccacggc ccacccccga cctcagccac cccgccatgg cggtcaacct ggacgcctgc
     3661 atccagtgca cgcgctgcgt gcgcgcctgc cgcgaggcgc aggtcaacga cgtcatcggc
     3721 tacgccggcc gcggggccga ggcgcgcatc gtgttcgacc tggacgaccc catgggcgcc
     3781 agcagctgcg tggcctgcgg cgaatgcgtg caggcctgcc ccaccggcgc gctcatgccc
     3841 aagacccaca tcggcccgca gcgggtggac cgcagcgtcg attcggtctg cccgttctgc
     3901 ggcgtcggct gccagatcac ctaccacgtg cgcgatgaaa agatcgtcag cgtgagcggc
     3961 cgcaatgggc ccgccaacgc cggaaggctg tgcgtcaagg gccgcttcgg ctttgactac
     4021 gccgccagcc ccgaccggct gacgcgcccg ctgatccgca tcgccggcgt gcccaaggac
     4081 ccggcgcact gtcagcagcc gcccgactgg cgcacggtgt ttcgcgaagc cagctgggac
     4141 gaggcgctgg acgcggccgc cggcgggctg cgcgcgctca agtcgcggca cggccccagg
     4201 gcgctggcag gcttcggcag cgccaagggc acgaacgaag aggcctatct gttccagaag
     4261 ctggtgcgca ccggctttgg cagcaacaac gtcgaccact gcacccggct gtgccacgcc
     4321 agcagcgtgg cggcgctgct ggagggcgtg ggctcgggcg cggtcagcaa cccggtgcgc
     4381 gatgtcgaac acgccgagct gatcctcgtc atcggcgcca atcccaccgc caaccacccg
     4441 gtggccgcca gctggatgaa gaacgccgcc cggcgcggca cccgcatcgt gctggccgac
     4501 ccgcgcgcca ccgacatcgc gcgccactgc tggcgcgtgc tgcagtttcg ccccggcagc
     4561 gacgtggcgc tgctcaacgc cctgctgcac gtcatcgtta ccgaggggtt gtgcaacgaa
     4621 tcgttcctgc gccagcgcgc cgaccacttc gccgcgctgg ccgccaacgt gcagggctac
     4681 agccccgagg ccatggcgcc gatctgcggc atcccggccg aaaccctgcg cgaggtggcg
     4741 cgcgcctatg ccacggcgcg ctcggccatg atcctgtggg gcatgggcgt gagccagcac
     4801 gtgcacggta ccgacaacgc gcgctgcctg atcgcgctgg ccacctgcac cggccagctc
     4861 gggcgccccg gcaccggcct gcacccgctg cgcgggcaga acaacgtgca gggcgccagc
     4921 gacgccggcc tgatccccat gatgtacccc aactaccagc gcgtggacag ccccgatgcg
     4981 cgcacctggt tcgaggactt ctggggccag ccgctggacc ccgaacccgg ctacaccgtg
     5041 gtcgaaatca tgcgccaggc gctggccgcc gacgacgacc cgcacaaggt gcgcggcatg
     5101 tacatcatgg gcgaaaaccc ggccatgagc gaccccgacc tgaaccacac gcgccacgcc
     5161 ctggccagcc tgtcgcacct ggtggtgcag gacatcttcc tgaccgagac cgcctggctg
     5221 gccgacgtgg tgctgcccgc cagcgcctgg cccgaaaaga ccggcagcgt cagcaacacc
     5281 gaccgcatgg tgcagctggg ccggcaagcg ctggcgccgc cgggtgaagc acgcgccgac
     5341 ctgtggatca tccagcagct ggcgcagcgc ctggggttgc cctggaacta tgaaaacgat
     5401 agctgctcgc gcttgccaga caagcgccag gggccgtttt tccttgaaga accgcacggc
     5461 gtcgcggcgg tctatgaaga aatgcgccgc gccatgcacg gcgccatcgc cggcatcggc
     5521 tgggaccggc tggtgcgcga atccagcgtc acctacccct gcctgagcga ggacgacccc
     5581 ggccagccga tcgtcttcac cgagcgcgtg cccacgccca acggccgcgt gcaactggtg
     5641 ccggccgaca tcatcgcgcc cgacgagcgc cccgacgccg actaccccat ggtgctgatc
     5701 accggccgcc agctcgagca ctggcacacc ggcgccatga cgcgccgcgc cgtcgtgctg
     5761 gatgcgctgg agcccgcgcc caccgtgtcc atgcacggcg acgatctggc gcggctgggc
     5821 ctggtggcgg gcgagctggt gcgcgtgagc tcacgccgcg gccgggtctg cctgacggcg
     5881 cggcgcgacg acggcacgcc ggtgggcggc gtgttcatcc ccttcgcctt tcgcgaagcc
     5941 gccgccaacc tgctgaccaa cgccgcgctc gacccctttg gcaagatccc cgagttcaag
     6001 tactgcgcgg tgcgggtgga gccgctggcg tgacggtgca cctcacgcgc gccagcgcgc
     6061 cgctcaccga acgcgtgctg gcggtcgacg agctgggtca ggcggtcgag gtgtcgatcc
     6121 cggccgagcg ggcgctgacg ctgtacgtcg acaagcgcga gctggtcacg ctgatgaccc
     6181 tgggcgccca ccccgaatgg ctggtgctgg gctatctgcg caatcagcgg ctgctggcgg
     6241 ggccggccga gctggcttcg actacggtgg actgggacgc tggcgcggcg gcggtgcgca
     6301 cgcggcacgg catcgccgac ctggagatgc gcacgcgccg gcgcaccgtc accagcggct
     6361 gcggtcaggg cagcgtgttc ggtgacctga tggccgatct ggaggcgctg gacctcggcg
     6421 ccggccggct ggcgctggac gatctgtacg gcctgctgaa cgccatccgg ctgcaggaga
     6481 gcacctacaa atcggccggt tcggtgcacg gctgcgcgct gttcgagggg cagcgcatgc
     6541 tgctgttcgt cgaggacgtg ggccgccaca acgccatcga caccatcgcc ggctggatgt
     6601 ggctgcaagg ggacggcggc ggtctgcgcg gcgacgacaa agtgttctac accaccggcc
     6661 gcctgaccag cgagatggtg atcaaggcgg cccagatggg cgtgccgatc gtggtctcgc
     6721 gcagcggcat cacgcagatg ggtctgcaac tggcgcgccg gcttggcctg tgcgccgtcg
     6781 ggcgggcgct gaaccggcgc ttcctgtgct acagcgcgcc cgagcgcctg ggcctgccgg
     6841 tgccgccgca cggcgcctga gcgacgtcag ccctggccct gcatcgcgcg gatcagcgcc
     6901 tgcgcctcgg cgccttcggg gatcgggcga ccgctgtcgc gccactcgac ggcggacttg
     6961 aagccgtgct gctgcgcatg gcggcggaac cacacgccct cggggttgtg gcgcgtgatg
     7021 ccgtcgaaca ccgtggccag catctgcgtc tgctccagcc ccatggtcag catgacctgg
     7081 ttgaccacca gcttgtgcat ctgcaggtga ccgcgcggca cgccggcgat gcgtgcggcc
     7141 aggcgcaggg tggcggcttc cagctcggcg gccggcaccg cctggttggc caggccccag
     7201 tcggccgcgg tgcggccgtc gatcacgtcg ccggtgaaca tcagttgctt ggcgcgcgtc
     7261 gggcccaggc ggtaggtcca catggcggtg gtggggcagc cccacacgcg cgtgggcatg
     7321 tagccgatgc gtgcatcttc ggccatcacc agcaggtcgc agcacagggc gatgtcgctg
     7381 ccgccagcca ccgccgcgcc atgcaccttc gcgatggtgg gcttggggca gcgccacagg
     7441 ctcatgaaat cttcggtgaa gcgcttcatg gcgcggtagt cgagcgccgg gtcccagggg
     7501 gccgattcct gctggcaggg gtgttcgatg tgttgctcgg cggtggccgc caggtcgtag
     7561 ccaccgcaga agcccttgcc cgcgccttcg accacgatca catgcacctc gtcgtcggcc
     7621 accgcccagt cgaccgcggc acggatctcg cccggcatgt cgtcgttgat ggcgttcagg
     7681 cgctccgggc ggttgagcag caggcgcgcg atgcgcggct gggccgggtc tttgtcgatg
     7741 cgcagggtgc tgaagatggg catggggctc tcctgagggc gggcgtccat ggtaggcccg
     7801 ccgcgcgcgg gcggctgtcg ctttgcgtac agtgcggggc atgaacctgt cgtcctggac
     7861 tctgggctgg cgcacgctgt ggcgcgattt gcgcgccggc gagctgcgcc tgctgatggt
     7921 ggcggtcacg ctggcggtgg cggcgctcag cagtgtgggc ttttttgccg accggttgca
     7981 gggcggcctg cagcgcgacg cgcgccagtt gctgggcggc gatgtggtgc tggtgagcga
     8041 tcacgcgccg gcgcccggct ggacgcagcg cgccgccgcc gaggggctgg acagcgtact
     8101 cacgctgggg tttcccacca tgggccgggc gcccgaggcg cagggcggcg ccacccggct
     8161 ggtggccctg aaggcggtcg agcgcggcta cccgctgcgc ggccggttgc gcctggcgca
     8221 gcacgcgcag gaccgcgccg gcacgccgac cgacgccgtg ccggcgtccg gtcaggtgtg
     8281 ggtcgatccg tcgctgctgg aagcgctggc gctgcggcgc ggcgacaccc tgctgctggg
     8341 cgacagcgcg ctgcgcatca ccgaactgat cgcgctggag cccgaccgcg gcaccggctt
     8401 catgagcttc gccccgcgcg tgctgatgaa cgccgccgac ctgcccgcca ccggcctggt
     8461 gcagccggcc agccgcatca cctggcgcct ggcgctggcc gggcccgagc cggcggtgca
     8521 gcgctaccgc gcctgggccg agaccgagct ggcgcgcccc ggcatggccg gcctgcggct
     8581 ggagacgctg gacaccggtc ggcccgagat gcgcaccacg ctggatcggg ccgccaagtt
     8641 cctcaacctg gtggcgctgc tggcggcgct gctgtcggcg gtggcggtgg cgctggcggc
     8701 gcgcgcgttc gcggcgcgcc acctggacga ttgcgccatg ctgcgcgtgc tgggcctgcc
     8761 acagcggcgc atcgccggcg cctacgtgac cgaattcggc ctggtgggtc tgagcgccag
     8821 tctggccggg gtgctgatcg gctggctcgt gcaccacatc ttcgtggccc tgctggccgg
     8881 cctggtggac agcgcgctgc cggcgcccgg cccgtggccg gcgctgctgg gcctgggcat
     8941 cggcatgacg ctgctggtgg cctttggctt gccgccggtg ctgcaactgg cgcaggtacc
     9001 gccgctgcgc gtgctgcggc gcgacgtcgg tgccctgcgg ccggcgtcgc tggcggtgct
     9061 gggcctgggc gtggccggct ttgccgcgct gctgctggcg gtcagcgccg atctgacgct
     9121 gggtctgatc gcggtcggcg gcttcgccgt ggcggtggcc ctgttcgccc tgctgtcctg
     9181 ggtcgcggtg cggctgctgc ggcggtgggt ccacgagcag cgtgcgccgc gctggctggt
     9241 gctggccacg cgccagctgt cggcgcggcc ggcctacgcg gtggtgcagg tcagcagcct
     9301 ggcggtgggc ctgctggcgc tggtgctgct ggtgctgctg cgcaccgatc tgatcgccag
     9361 ctggcgtgcc gccaccccgg ccgatgcgcc caaccgcttc gtcatcaaca tcatgccgga
     9421 gcagggcgag gcgtttcgcg ccgtgttgcg gcaggccggc gtcacgcgca tggactggta
     9481 cccgatgttc cgcggccggc tggtggccat caatggccag ccggtgaatc cggccgacta
     9541 ccccgaggag cgcgcgcgcc atctggtcga tcgcgagttc aacctctcgc acgcggccga
     9601 acgaccggcg cacaacgaca tcgccgccgg ccactggacc accgacgagg ctggcgccat
     9661 cagcgtcgag cagggcctgg ccgagacgct gcgcctgcgg ctgggcgacc ggctgcgctt
     9721 cgacgtcgcg ggcatgccgc tcgaggcgcg catcacctcg ctgcgcaagg tcgactgggc
     9781 ctccatgcac gtcaatttct ttgccatgtt cccgcgggcc gacatgcccg acgtgccggt
     9841 cacctggatc gctgcctacc gcgcgcccgg acagccgggc ttcgaccacg cgctggtgcg
     9901 ccagtttccc aacatcacac acgtcgacat ggagcagtcc atcgcccagg tgcagcgcgt
     9961 gctggaccag gtggtgcggg cggtcgagtt cctgttcggc ttcacgctgg cggctgggct
    10021 gatcgtgctg tttgccgccg tcagcgccac gcgtgaggag cgcgcgcgcg aattcgccgt
    10081 catgcgcgcc gtcggcgccg gcagtgcgct gctggcgcgt gtgcagcagg ccgaactggt
    10141 tggcgtcggc ttgctggcgg gggtgctggc cagcgcggtg gcgatgggca tcggctgggc
    10201 catggcgcgc catgccttcg acttccactg gaccgcctcg ccctgggtgc cgctgctggg
    10261 cggcgtggct ggcgccgggc tggcactggc ggccggctgg tggggcttgc gcgaagtgct
    10321 gcagcggccc gtggtgcaga cgctgcggcg ggtcgcggag tgagcggccg gatgcgtcgg
    10381 ccgcgctgac tggggccgcc gctgcacagg cgccggggca tggccgcgtg tgtcgcgcaa
    10441 gaaaaaaccc gcgcaacgcg cgggtcttcg gggcgccgtt ggctcaaccg atgtcgccgg
    10501 tggccgggcg gaaaccctgc gccatggcgt cgcgcagttc ggccaccacc gtttcgcggt
    10561 ccaccggctg cggcgtgggc gccgtggtct gactcccgtt gccctcaccg ctggccggtg
    10621 gcgattggat cgccatcgcc atcacctcag cgcgcgtcag gtcggagggg cctcggatcg
    10681 ggtcgttgaa gaacggacct tcgccgctgg cgggcatggc ggccgatgcg gcgccggcga
    10741 tcagcgtcca cgccagggtc gaagccaagg gaaagaaacg tgcggacatg atgaaactcc
    10801 ttgaaaggct catgaagcga tggcaacaga agaggcaatg cgtaagtacc accttgttcc
    10861 gtgaaagagg ccccgctcca atggcctctt ccatgcgacg tactgtgcga cttgcaaggc
    10921 gctgaataaa gtcgtcgcat ggaatgtcga ccattccagg ccgtgaacaa tggatacgct
    10981 ggtgctcctg cgcacgtttc gagaggtggc cctgcgccac aacttctcga ctgccgcgcg
    11041 cacccttgat ctggcgccgg ccacggtcag caagtacgtg gccgaactgg aggcgcgctt
    11101 tcacctgcgc ctgttccatc gcagcacgcg ccgggtgacg ctgaccgacg ccggccagtt
    11161 gctgctggat cattgcgcgc aggtgatcga cctgatcgat cggacgcacg acgccatgaa
    11221 cgagcgtgcg cagcggccca gcggacggct caatctgacg gcgccgcatg ggctgacgcg
    11281 cacgatcctg gtgcgccgtc tggcggattt catgctgcag cattcggatg tgtcgatcaa
    11341 cctgaacctg accaatcgca tggtcgatct ggttgaagat ggtgtcgacg tcgccttccg
    11401 cgtcggggcg gtggccgaca acagcctgat cgtgcgccgc ctgctgcccc tggagatggt
    11461 ggtggcggcc acgcccgcct actggcgcgc gcacgggcgt cccgaacacc cgcgcgaact
    11521 ggccacccat cgcacgctgg ccatggcacc gacgggcgag acgccgcact ggcagttcag
    11581 catcggcggc aagctgttcg acctgccgct gcacccggtg ttctgtgcca gcgactcctc
    11641 accgctggtg ccgctggcgc tgcagggcgt gggtgtgctg cgtggctctc gcatgctgct
    11701 gggcgaatgg atcgacgatg gcaggctgga gccgctgttt gcagcgtatt caccgcgcaa
    11761 cctgtggctg tacgccgcct atgcccagcg ccgccacccc agtgcggcgt tgcgggcgct
    11821 gctggatttt ctcgagcagc agatgcgtcc caccgtcccg tcggtcccgc atgccgtttc
    11881 gccgaggctg cactgaacct gaactcggcc atggagcgcg caatggtcgg tctcgggcca
    11941 agcgatctgc atcgaatggc cggtgtggct tgtggggata agcgccggca gctactgaaa
    12001 tcatagtaaa ctggggcatg gcccagctct tcaccgtgca ccccgacaac ccgcagccgc
    12061 gcctgctcaa gcaggcggcg gcgttgctgc acggcggcgg cgtgctggcc gtgcccaccg
    12121 attccagcta cgccctggtg tgccacctcg acgacaaggc ggcggtcgat cggctgcgcc
    12181 gcatccgcca ggtcgacgag cgccaccacc tgaccctgct gtgccgcgac ctgagcgagc
    12241 tggccagcta cgcccgcgtc gacaacgcgc agtttcgccg cctgaagagt ggcacgccgg
    12301 ggccgttcac cttcattctg gaagccagca aggaagtgcc gcgtcgcgtc agccatccgc
    12361 agcgcaagac catcggcctg cgcgtgcccg atcaccccgc actgcgtgaa ctgctggcgc
    12421 tgcatggtgc gccgctgctg gccaccacgc tgatcctgcc gggggagggc gagccgctga
    12481 acgacgccgg ggcgatcctg gagcagctgg gccatcagat cgccggcgtg atcgacgccg
    12541 gcgcctgccc gctggcgccc accaccgtgg tcgacctgag tggcgacgcg cccgaggtgc
    12601 tgcgccgcgg gaggggcgat ccggcgcggc tggggctgtg attcggcgcc tggtcacggt
    12661 ctgagacaat ccacgccgtg gacttcgcca acatcattca gaccctcgcc ctctatgccc
    12721 tgccggtgct gtttgccatc accgtgcacg aggcggcgca cggttacgcg gcgcgccatt
    12781 tcggcgacgg cacggcggcc ttcctgggcc gcgtcacgct caatccggcg cggcacatcg
    12841 accccatcgg caccatcgcc atgccgctgt ttctgtacat cgccaccggt ggtgccttcg
    12901 tgttcggcta cgccaagccg gtgccggtca acttcggcca gctgcgccac cccaagcgcg
    12961 acatggtgtg ggtggcgctg gcggggccgg cgtccaacct ggcgatggcg ctggcctggg
    13021 gggcgctgct gatcgtgctc gacggcgtgg gggtgaccga gccgttcttt ctcaagatgt
    13081 gccaggccgg cgtgctggtc aacgtggtga tcggcgtgtt caacctgttt ccgctgccgc
    13141 cgctcgatgg cgggcgcatc ctggtggggc tgctgccgcg cgggccggcc ctggccctca
    13201 gccgcatcga gccctggggc tttttcatcg tcatggcgct ggtggtggcg ggcgtcatca
    13261 gcaacctgtg gatgcggcca ctgatggcac tgagctttca tctgctcgat tccattcttg
    13321 cgccgctgca ggcgctgttc ggctgatcgc atggcttctt ctgtttcgcg ccgggtcctc
    13381 accggcatca ccaccaccgg caccccgcac ctgggcaact acgtcggctc gatccgtgcc
    13441 gccgtgcgcg ccagccgcga gccgggggtg cagagctttt acttcctggc cgactaccac
    13501 gcgctgatca agtgcgacga cccggcgcgc atccagcgca gcacgctgga aatcgccgcc
    13561 agctggctgg ccgccgggct ggatcccgag caggtcacct tctaccgcca gtccgacatc
    13621 ccggagatcc ccgagctcac ctggctgctg acctgcgtca ccggcaaggg tctgctcaac
    13681 cgcgcccacg cctacaaggc catgctcgac aagaacgccg ccaccggggc cgatccggac
    13741 agcgacgtca ccgcaggcct gttcatgtac ccggtgctga tggcggccga catcctgatg
    13801 ttcaaggcgc accaggtgcc ggtcgggcgc gaccagatcc agcacatcga gatggcacgc
    13861 gacatcgccg ccagcttcaa ccacctgtat ggcgaactcc tgaccctgcc cgaagcgctg
    13921 atcgacgaca acgtggccct gctgcccggc ctggacgggc gcaagatgag caagagctac
    13981 gacaacacca tcgccctgtt cggcccgcgc gagcagctgc gc
//
LOCUS       DAOOOG010000051        13980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4736_length_13980_cov_24.546858, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000051 DAOOOG010000000
VERSION     DAOOOG010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13980
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4736_length_13980_cov_24.546858"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2353)
                     /locus_tag="PK242_05675"
     CDS             complement(<1..2353)
                     /locus_tag="PK242_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOZ93540.1"
                     /translation="MQSELASTLYDSGPPRTRWQRLLKGQDPIAQLAPLAAVLLFLAA
                     IVSAFWYLRLEETRREQEAVKRDVEYSQQRLRLRLLERQEQLMRLARELADREMTALQ
                     FDARAEAMVAQYPELRAVSWIDGQRRLRTTQTSPSVSPQPDWIADGRLKPGEVSEGYS
                     LAQDLRQAVYNQPLAVEDADAVLYLHVPITPRAQFAGVVLAEYSVDGLLRYGVPSEVL
                     AKYAVSLLDAKGQLLAGQTIPDRTGLSEYLPWSEQVNAFDSPVSPVGNGLVLRAQAWR
                     TSQGVVGGTLFWLVAVLASMTAWMLIANWRQSQRRQQAQHALLAETSFRRAMENSMLT
                     GMRALDLQGRIIYVNPAFCQMTGWSEEELLGQAEPFSYWPDADRAELSSRLKEELRGT
                     ADPAGFQMSVKRKDGSLFDARLYVSPLIDATGKHAGWMASMTDITEPNRVRRQLSASY
                     ERFTTVLEALDASVSVAPLGSQELLFANKLYRQWFDGTTTGHLKLVERAGTPETQASE
                     ESLDTVDSLVGLPVDSLANAPLAEHAEVYIERLNKWLEVRSRYLNWVDGRLVQMVIAT
                     DITARRQAEEQAAAQAERAEAASRLITMGEMASSVAHELNQPLTAISNYCTGMISRVR
                     SGQISRDDLLGALDKTARQAQRAGQVVQHIRSFVKRSEPRRAWAEVTPMVEQVLELAE
                     IELRRRNVRLVRQIATDLPALYVDPILIEQVLMNLIKNAAESIDVAQRAPGQRLIELR
                     VVQRDIEDQPTVQFSVTDNGRGLAPEALARLYEAFFSTKSDGLG"
     gene            2582..5278
                     /gene="aceE"
                     /locus_tag="PK242_05680"
     CDS             2582..5278
                     /gene="aceE"
                     /locus_tag="PK242_05680"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOZ93541.1"
                     /translation="MSNNPQAQSAAELDAQETREWLDALTAVIEREGPERAHALLEGL
                     LEHARQASIDLPFSANTGYVNTLEPEQEARCPGNIAIEKRLRAYMRWNAMAMVVRANR
                     IDPPEGGSLGGHIGSFASLASMWGAGFNHFWHAESEHHGGDCIYFQGHSAPGIYARAY
                     LEGRINEEQLENFRQEVKGKGLSSYPHPHLMPGFWQYSTVSMGLGPMMAIYQARFMKY
                     LHARGIANTEGRKVWVFLGDGEMDEPESRGAITLASRENLDNLIFVINCNLQRLDGPV
                     RGNGKIIQELEGDFRGAGWNVIKLLWGKGWDDLLRRDKDGKLRQLMMETLDGDYQTFR
                     AKDGAYIRQHFFGKYPETAKLVEHLTDDEIWDLRRGGHDPEKVYAAFHAANAHQGEPT
                     VLLVKTVKGYGMGRAGEGRNTVHQTKLLDDEDIRYIRDRFNIPIPDSELDKLPYYKPA
                     DDTPEMKYLHERRQALGGYLPHRREKADESFTIPSLETFKAVIEPTAEGREISTTQAY
                     VRFLTQLLRDKALGPRVVPILVDEARTFGMEGLFRQIGIYNPKGQLYTPQDREQVMYY
                     KESVDGQILQEGITEAGGMCSWIAAATSYSTNNRIMIPFYIYYSMFGFQRFGDLAWAA
                     GDMLARGFILGGTSGRTTLNGEGLQHEDGHSHILAGTIPHCLTYDPSFAHEVAVILHD
                     GLKRMVEKQENVFYYITLLNENYAMPGLTPGTEQQILQGMYLCKPGAEGERRVQLLGS
                     GSILRESLEAQKLLATDWGVQADVWSCPSFNQLTREGQDVERWNLLHPLEQPRVPFVA
                     RQLAGHPGPVVASTDYLKNYAEQIRPFVPKDRPYIVLGTDGFGRSDFRARLREHFEVD
                     RHYIAVAALKGLADAGALPRQKVADAIRKYGIDTEKINPLHA"
     gene            5291..6928
                     /gene="aceF"
                     /locus_tag="PK242_05685"
     CDS             5291..6928
                     /gene="aceF"
                     /locus_tag="PK242_05685"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HOZ93542.1"
                     /translation="MALVEVKVPDIGDFSDVAVIELLVAPGDTVKLEQSLITVESDKA
                     SMEIPSSAAGVVRELKVKIGDKVSQGSLVLLLESADSASKPAPAQAQQAPAAIDTIAN
                     AAPPAAASVASGPVEVRVPDIGDFKDVAVIEVFVQPGDTIRVEQSLITVESDKASMEI
                     PSSAAGVVREMKVKVGDKVNQGDLLALLEGAAPVAAAPAAAAPANAPAPAAVSAPAAP
                     PLAAAPAHEPTRAPLGLPHASPSVRKFARELGVPLAEVKGSGPKGRITQEDVQAFTRA
                     VMSGAQRTQAQGAAGVSGGGVTGGGELNLIPWPKVDFAKFGPIERKELSRIKKISGAN
                     LMRNAVMIPAVTNHDDADITDLEAFRVSTNKENEKSGVKVTMLAFLIKACVAALKKFP
                     EFNSSLDGDALVYKNYWHIGFAADTPNGLVVPVIKDADKKGVLQISQEMGELAKKARD
                     GKLGPADMSGATFTISSLGGIGGRYFTPIINAPEVAILGVCRSNIEPVWNGEKFKPRL
                     MLPLSLTWDHRVIDGAAAARFNAYLGQILADFRRVLL"
     gene            6942..8798
                     /gene="lpdA"
                     /locus_tag="PK242_05690"
     CDS             6942..8798
                     /gene="lpdA"
                     /locus_tag="PK242_05690"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOZ93543.1"
                     /translation="MAVIDIKVPDIGDFADVGVIELLVKPGDTVKVEQSLITVESDKA
                     SMEIPSSHAGVVKELKVKLGDKVSEGSVILTLEAAGASATSPAGAGEGRDEGGVQAGA
                     QPSPSPQPPPASGRGREPAPIASSFAGTADLDCDVLVLGGGPGGYSAAFRAADLGLKV
                     VLVERYATLGGVCLNVGCIPSKALLHVAAVMDEVGHLAALGVDFGKPKLDIDKLRSHK
                     EKVIAKLTGGLGAMAKMRKVTLVRGYGSFVGANHLQVEETTGSGQDKTGSKRVVQFKR
                     AIIAAGSQAVRLPFMPEDPRVVDSTGALALKAVPKKMLILGGGIIGLEMGTVYSTLGA
                     RLDVVEMLDGLMQGADRDLVKIWQKMNAKRFDHIMLKTKTVGAKATPAGIEVTFASAE
                     EGGTAPAPQVYDLVLQAVGRTPNGKKIAADKAGVAVTERGFINVDIQMRTNVPHIFAI
                     GDIVGQPMLAHKAVHEAHVAAEVIAGELKGDEALARAAFNARVIPSVAYTDPEVAWVG
                     LTEDQAKAQGIKVKKGLFPWSASGRAIANGRDEGYTKLLFDEATHRIVGGGIVGTHAG
                     DMIGEVALAIEMGADAVDIGKTIHPHPTLGESIGMAAEVAHGSCTDVPPQRK"
     gene            9215..9826
                     /locus_tag="PK242_05695"
     CDS             9215..9826
                     /locus_tag="PK242_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOZ93544.1"
                     /translation="MAWHTWLIYFVAALGLSLSPGPNGLLALTHGALHGRRKTLFTVA
                     GGALGFATVIALSMFGIGALLQASLVWLTVLKWVGGGYLVWLGVQVWRAPPIAALALQ
                     PQGEVNGWSLFRQGALSALTNPKGILFFAAFLPQFIDPARSLLLQYFIMAGTFVAIEF
                     ITEVLIASMAQRISGWLARVGRGFNRVCGGIFAAIGVALPLRA"
     gene            complement(9988..10824)
                     /locus_tag="PK242_05700"
     CDS             complement(9988..10824)
                     /locus_tag="PK242_05700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOZ93545.1"
                     /translation="MSTISPSAVTALVPAYQAAEFIQPTLDALSAQTHPALHVLVSVD
                     LCDDDTQAICERHALRDPRFRVVRQTSRLGWVGNSNFLLSQAQGDYALFAFHDDLLAP
                     TYIARLAAELDADPQACVAFSDVLLTDTQGGQEFWSYPTLDGERSAFIRGVRVLERRG
                     KWWVPNRGLFRLSMARQIGGLKKHGAGEFSADWPWVLHLALLGSFVRVPELLCHKFYK
                     PGSLSRSWDFSQPQYFEVHAACMREIWNSSLGTQEKIALALPLMQWLERHRHLAMTPP
                     ST"
     gene            complement(10821..12137)
                     /locus_tag="PK242_05705"
     CDS             complement(10821..12137)
                     /locus_tag="PK242_05705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ93546.1"
                     /translation="MSRSVPDSRVWVVGAGLQGCAIALELAHRGQPVVLLDQDALPMN
                     RASLRNEGKIHLGLIYAADASGATARVQLRGALVFQHLLERWLGRPVAAALPLSTPFA
                     YLVARDSVLPAPTLARHFDRVQALYKHWMTQHPEASYLGQRPTRLAWPASRDEHQGLV
                     LAPGESVFTTAERAIDTDALAAALRRAVITHPLIDWLPGRRITRFAPAPDGVTLHGQV
                     LGSGGEPLEPLEACARQVVNATWEQRLGLDASMGLQPAPGWLHRLKYRVIVRLPAGWE
                     HLSSVTMVLGRYGDVVMRPNGTAFVSWYPEGLRGWSEALVPPPEWDGPCRGDVPPDQA
                     QALAEAILRAADRWYPGLHRATVLNVDAGAIFAYGREDVDRLDSGLHARSFNGPRGDA
                     RYLSVDSGKLTTAPWYAVQAADLLLGQRTALAQTWTDVDPDGAAAP"
     gene            complement(12244..13416)
                     /locus_tag="PK242_05710"
     CDS             complement(12244..13416)
                     /locus_tag="PK242_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOZ93547.1"
                     /translation="MNSERTPAVPSRLPNVGTTIFTVMSALATQHQAVNLGQGFPDFD
                     CDPALVDAVTAAMRQGLNQYPPMPGVPALREAIAAKIEALYGRHYDAGAEITVTAGAT
                     QAILTAILCCAGPGDEVIVLEPCYDSYVPNIELSGARVVRVPLTPGSFRPDFDRLAAA
                     LSPRTRAILINSPHNPSGMVWSAQDMLRLQELLAPTDALVISDEVYEHMVYAPLRHES
                     AARYPGLAARSFIVSSFGKTCHVTGWKVGYVAAPAILTAEFRKVHQFNVFTVNTPMQY
                     GIAAYMQDPRPYLELPSFYAAKRDLFRAGLAQTRFRLLPCEGTYFQCAAIDELSVPER
                     DLSEADFCQWLTREIGVAAIPLSAFYGDGFDQKVIRFCFAKQDGTLDNALARLARL"
     gene            complement(13406..>13980)
                     /locus_tag="PK242_05715"
     CDS             complement(13406..>13980)
                     /locus_tag="PK242_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOZ93548.1"
                     /translation="ISLHQGAPDQVARMVIEETAEIGVATESLASYPELVTLPCYEWQ
                     HVLVLPATHPLSKKERLALEDIAAQPLITYHPSFTGRTRVDAAFAQRQLTPRVVLEAI
                     DSDVIKTYVRLGLGIGIVAEMAMADDRQGDLVARPLGPLLGQNVARIALKRGAWLRDF
                     VYHFASLLSDRLDKDLILKAMSGHLNDYEL"
BASE COUNT         2400 a         4621 c         4763 g         2196 t
ORIGIN      
        1 tgcccaggcc gtcgctcttg gtggagaaga aggcttcgta cagccgcgcc agcgcctcgg
       61 gcgccagacc gcgcccgttg tcggtcacgc tgaactgcac cgtgggctga tcttcgatgt
      121 cgcgctgcac cacgcgcagc tcgatcaggc gctggcccgg cgcacgctgg gcgacgtcga
      181 tcgattcggc ggcgttcttg atcaggttca tcagcacctg ctcgatcagg atcgggtcca
      241 cgtacagggc cggcaggtcg gtggcgatct ggcgcaccag ccgcacgttg cggcggcgca
      301 gttcgatctc ggccagctcc agcacctgct cgaccatcgg cgtcacctcg gcccaggcac
      361 ggcgcggctc gctgcgcttg acgaagctgc ggatgtgctg caccacctga ccggcgcgct
      421 gcgcctggcg ggcggtcttg tcgagtgcgc cgagcagatc gtcacggctg atctgcccgc
      481 tgcgcacgcg cgagatcatg ccggtgcagt agttgctgat cgccgtcagc ggctggttca
      541 gctcgtgggc gacgctggaa gccatctcgc ccatggtgat caggcggctg gcggcttcgg
      601 cgcgctcggc ctgcgcggcg gcctgctcct cggcctgccg gcgcgccgtg atgtcggtgg
      661 cgatcaccat ctgcaccagg cggccgtcca cccagttcag gtagcgcgag cgcacttcca
      721 gccacttgtt caggcgttcg atgtagacct cggcatgctc tgccagcggc gcgttggcca
      781 ggctgtcgac cggcagaccg accagggagt cgaccgtgtc caggctctct tcggacgcct
      841 gggtttcggg cgttccggcg cgctccacca gcttcagatg gccggtggtg gtgccgtcga
      901 accactggcg atacagcttg ttggcaaaca gcagctcctg gctgcccagc ggcgccaccg
      961 acaccgaagc gtccagcgct tccagcacgg tggtgaagcg ctcgtacgag gccgacagct
     1021 ggcgccgcac ccggttcggt tcggtgatgt cggtcatcga cgccatccag ccggcatgct
     1081 tgccggtggc gtcgatcagg ggcgagacgt acaggcgggc atcgaacagc gagccgtcct
     1141 tgcgcttgac actcatctgg aagccggccg ggtcggccgt gccgcgcagc tcctccttca
     1201 ggcgcgacga cagctcggcg cggtcggcgt cgggccaata gctgaacggc tcggcctggc
     1261 ccagcagctc ttcctcgctc cagcccgtca tctggcagaa agccgggttg acgtagatga
     1321 tgcgcccctg cagatcgagc gcacgcatgc cggtgagcat ggagttttcc atcgcccgac
     1381 ggaagctggt ttcggccagc agcgcatgct gcgcctgctg acggcgttgc gactggcgcc
     1441 agttggcgat caacatccat gccgtcatgc tggccagcac cgccaccagc cagaacagcg
     1501 tgccaccgac cacgccttgt gaggtgcgcc aggcctgggc gcgcagcacc agcccgttgc
     1561 cgaccgggga gaccgggctg tcgaaggcgt tgacctgctc gctccagggc aggtactccg
     1621 acaggccggt gcggtccggg atggtctggc cggccagcag ctggcccttg gcgtccagca
     1681 gcgacacggc gtatttggcc agcacctccg acggcacgcc atagcgcagc aggccatcga
     1741 ccgagtattc cgccagcacc acgccggcga actgcgcgcg cggcgtgatc ggcacgtgca
     1801 ggtacagcac cgcatcggcg tcctcgacgg ccagcggctg gttgtacacg gcctggcgca
     1861 gatcctgtgc cagactgtag ccctcgctga cctcgccggg cttgagccgg ccgtcggcga
     1921 tccagtcggg ctggggcgac acgctgggcg aagtctgcgt ggtgcgcagc cggcgctggc
     1981 cgtcgatcca gctgaccgcg cgcaactccg ggtactgcgc caccatggcc tcggcgcggg
     2041 cgtcgaactg cagcgccgtc atttcgcggt cggccagctc gcgcgccagc cgcatcagct
     2101 gctcctggcg ctccagcagg cgcaggcgca ggcgttgctg gctgtattcc acgtcacgtt
     2161 tgacggcctc ctgctcgcgc cgcgtttcct ccaggcgcaa gtaccagaag gccgacacga
     2221 tggccgccag aaacagcagc accgccgcca gcggggccag ctgggcgatc ggatcctgac
     2281 ctttgagcag gcgctgccag cgtgtccggg gcggaccgga gtcgtacagg gtggaggcaa
     2341 gttcggactg catgcatgtc cagtgtacgt ggctgaagac ggccgtggcg gcgtgggtgc
     2401 gcactccggc ccgtcagggc cgtcgaggcg ctggctggag atgcgctgcg gacaagggtg
     2461 tgaccgatca gaattgcgta ttatgagata acaaattact atttgaaatt gcgccgattt
     2521 gtgcgatcat cgccgctggt tgggtcacaa tgacttcgac aacatgagag gagacgcgaa
     2581 gatgtccaac aatccacagg cgcagtctgc tgccgaactg gatgcccagg aaacccgtga
     2641 atggctggac gcgctgacgg cggtcatcga gcgcgagggc cccgagcgcg cccacgcgct
     2701 gctggaaggc ctgctggagc acgcccgcca ggccagcatc gacctgccgt tttccgccaa
     2761 caccggctac gtcaacacgc tcgagcctga gcaggaggcg cgttgcccgg gcaacatcgc
     2821 gatcgaaaag cgcctgcgcg cctacatgcg ctggaacgcc atggccatgg tggtgcgggc
     2881 caaccgcatc gacccgcccg aaggcggcag cctgggtggc cacatcggct cgttcgcttc
     2941 gctggccagc atgtggggcg ccggcttcaa ccatttctgg catgccgaga gcgaacacca
     3001 cggcggcgac tgcatctact ttcagggtca cagtgcgccc ggcatctatg cccgcgccta
     3061 cctggaaggt cgcatcaacg aagagcagct ggaaaacttc cgccaggagg tcaagggcaa
     3121 gggcctgtcc agctacccgc acccgcacct gatgcctggc ttctggcagt actccaccgt
     3181 gagcatgggc ctggggccga tgatggcgat ctaccaggcg cgcttcatga agtacctgca
     3241 cgcgcgcggc atcgccaaca ccgaagggcg caaagtttgg gtgtttctgg gcgacggcga
     3301 aatggacgag cccgaaagcc gcggcgccat cacgctggct tcgcgcgaga acctggacaa
     3361 cctgatcttc gtcatcaact gcaacctgca gcgcctcgat gggccggtgc gcggcaacgg
     3421 caagatcatc caggagctgg aaggcgactt ccgtggcgcc ggctggaacg tcatcaagct
     3481 gctgtggggc aagggctggg acgacctgct gcggcgcgac aaggacggca agctgcgcca
     3541 gctcatgatg gaaacgctgg atggcgacta ccagaccttc cgcgccaagg atggcgccta
     3601 catccgccag catttcttcg gcaagtaccc cgagacggcc aagctggtcg aacacctgac
     3661 cgacgatgaa atctgggatc tgcgccgtgg tggccacgat ccggagaagg tgtacgccgc
     3721 cttccatgcc gccaatgcgc accagggcga gcccacggtg ctgctggtca agaccgtcaa
     3781 gggctacggc atgggcaggg ccggcgaggg ccgcaacacc gtgcaccaga ccaagctgct
     3841 ggacgacgag gacatccgct acatccgcga ccgcttcaac atcccgattc ccgacagcga
     3901 gctcgacaag ctgccctact acaagccggc cgacgacacg ccggagatga aatacctgca
     3961 cgagcgccgc caggccctgg gtggctatct gccgcaccgg cgcgagaagg ccgacgagag
     4021 cttcaccatc ccctcgctgg agaccttcaa ggcggtgatc gaacccaccg ccgagggccg
     4081 cgagatatcc accacccagg cctatgtgcg cttcctcacg cagctcttgc gcgacaaggc
     4141 gctgggcccg cgcgtggtgc cgatcctggt cgacgaggcg cgcaccttcg gcatggaggg
     4201 gctgttccgc cagatcggca tctacaaccc caagggtcag ctctacacgc cgcaggatcg
     4261 cgagcaggtg atgtactaca aggagtcggt cgacggccag atcctgcagg agggcatcac
     4321 cgaagccggt ggcatgtgtt cgtggatcgc ggcggcgacc tcgtactcga ccaacaaccg
     4381 catcatgatc ccgttctaca tctactactc gatgttcggg tttcagcgtt tcggtgacct
     4441 ggcctgggcg gcgggtgaca tgctggcgcg cggcttcatt ctgggcggca cctccggacg
     4501 caccaccttg aacggcgagg gcctgcagca cgaggatggc cacagccaca ttctggctgg
     4561 caccattccg cactgcctga cctacgaccc gagcttcgcg cacgaggtgg cggtgatcct
     4621 gcacgacggc ctgaagcgca tggtcgagaa gcaggaaaac gtcttctact acatcaccct
     4681 gctgaacgag aactacgcca tgccgggcct cacgccgggc accgagcagc agatcctgca
     4741 gggcatgtac ctgtgcaaac ccggcgccga gggcgagcgg cgcgtgcagc tgctgggctc
     4801 gggctcgatc ctgcgcgaat cgctggaggc gcagaagctg ctggccaccg actggggcgt
     4861 gcaggccgat gtgtggagct gcccgagctt caaccagctc acgcgcgaag gccaggacgt
     4921 cgaacgctgg aacctgctgc acccgctgga gcagccgcgc gtgcccttcg tggcgcgcca
     4981 gctggccggc cacccgggcc cggtggtggc gtcgaccgac tacctcaaga actacgccga
     5041 gcagatccgc cccttcgtgc ccaaggaccg gccctacatc gtgctgggca ccgacggttt
     5101 cggtcgcagc gacttccgcg cccgcctgcg cgagcacttc gaggtcgatc gccactacat
     5161 cgcggtggcg gcgctcaagg gtctggccga tgccggcgcg ctgcccaggc agaaggtggc
     5221 cgatgccatc cgcaagtacg gcatcgacac cgaaaagatc aacccgctgc acgcctgagg
     5281 gagacgagtc atggcactgg tggaagtcaa ggtcccggac atcggcgact tttcggacgt
     5341 ggcggtgatc gagctgctgg tggcgcccgg cgacaccgtg aagctcgaac aaagcctgat
     5401 cacggtggag tcggacaagg cgtcgatgga gatcccctcc agcgccgccg gcgtggtcag
     5461 ggagctcaag gtcaagatcg gcgacaaggt cagccagggt tcgctggtgc tgttgctcga
     5521 atccgcggat tcggcatcaa aaccggctcc ggcgcaggcg cagcaagcgc cagcagctat
     5581 tgatacaata gcaaacgcgg cgcccccggc ggctgcatcg gtggccagcg ggccggtcga
     5641 ggtgcgcgtg cccgacatcg gtgacttcaa ggacgtggcg gtgatcgagg tgttcgtcca
     5701 gcccggcgac acgatcaggg tcgaacaaag cctgatcacg gtggagtccg acaaggcctc
     5761 gatggaaatc ccctccagcg ccgccggggt ggtgagggag atgaaggtca aggtcggcga
     5821 caaggtcaac cagggcgatc tgctggcgct gctggaaggc gccgcaccgg tggccgcagc
     5881 gccggccgcg gcggcgccgg ccaacgcccc ggcgcccgcc gccgtgtcgg ccccggcggc
     5941 gccgccgttg gcggccgcac ccgcgcacga acccacgcgc gcgccgctcg gtctgccgca
     6001 tgccagcccc agcgtgcgca agttcgcgcg cgagctcggg gtgccgctgg ccgaggtcaa
     6061 gggcagcggc cccaagggcc gcatcacgca ggaagacgtg caggccttca cccgcgccgt
     6121 gatgagcggc gcgcagcgca cgcaggcgca gggcgcggcc ggcgtgtccg gtggcggcgt
     6181 caccggcggc ggcgagctga acctgatccc ctggccgaag gtggactttg ccaagtttgg
     6241 ccccatcgag cgcaaggagc tcagtcgcat caagaagatc agcggcgcca acctgatgcg
     6301 caacgcggtc atgatcccgg ccgtcaccaa ccatgacgac gccgacatca ccgacctcga
     6361 agccttccgc gtctccacca acaaggagaa cgagaagagc ggcgtcaagg tcaccatgct
     6421 ggccttcctg atcaaggcct gcgtggccgc gctcaagaaa ttccccgagt tcaacagcag
     6481 cctggacggc gacgccctgg tctacaagaa ctactggcac atcggctttg ccgccgacac
     6541 gcccaacggc ctggtcgtgc ccgtcatcaa ggacgccgac aagaagggcg tgctgcagat
     6601 cagccaggaa atgggcgaac tggccaagaa ggcgcgcgac ggcaagctgg gcccggccga
     6661 catgagcggc gccaccttca ccatcagcag cctgggcggc attggcgggc gctacttcac
     6721 gcccatcatc aacgcgcccg aggtcgccat cctgggtgtg tgccgctcca acatcgagcc
     6781 ggtgtggaat ggcgagaagt tcaagccgcg cctgatgctg ccgctgtcgc tcacctggga
     6841 ccaccgcgtg atcgacggcg ccgcggccgc gcgcttcaac gcctatctgg gccagatcct
     6901 ggcggacttc cgccgcgtgc tgctgtgaag gagacgcgga catggccgtg atagacatca
     6961 aggtgccgga catcggcgac ttcgccgacg tcggcgtgat cgagctgctg gtgaagcccg
     7021 gcgacaccgt caaggtcgag cagagcctga tcaccgtcga atccgacaag gcgtcgatgg
     7081 agattccgtc cagccacgcc ggcgtggtca aggaactcaa ggtcaaactg ggcgacaagg
     7141 tgagcgaagg ctcggtgatt ctgacgctgg aggccgcagg ggcctcggcc acctctcccg
     7201 ccggggcggg agagggcagg gatgagggtg gggttcaagc gggagcgcaa ccttcaccct
     7261 caccccaacc ccctcccgcc agcggcagag ggcgtgaacc cgcccccatc gccagcagct
     7321 ttgccggcac ggccgatctg gactgcgacg tgctggtgct gggcggcggc cccggcggct
     7381 attcggccgc ctttcgcgcc gccgacctgg gcctcaaggt ggtgctggtc gagcgctacg
     7441 ccaccctggg cggcgtgtgc ctgaacgtgg gctgcatccc gtccaaggcg ctgctgcacg
     7501 tggccgcggt gatggacgaa gtgggccatc tggccgcgct gggcgtggac tttggcaagc
     7561 ccaagctcga catcgacaag ctgcgcagcc acaaggaaaa ggtgatcgcc aagctgacgg
     7621 gcggcctggg cgccatggcc aagatgcgca aagttacgct ggtgcgcggc tacggcagct
     7681 tcgtgggcgc caaccacctg caggtcgaag aaaccaccgg cagcggccag gacaagaccg
     7741 gctctaagcg cgtggtgcag ttcaagcgcg ccatcatcgc cgccggcagc caggccgtgc
     7801 gcctgccctt catgcccgag gacccgcgcg tggtggacag caccggcgcg ctcgcgctca
     7861 aggccgtgcc caagaagatg ctgattctgg gcggcggcat catcggcctg gagatgggca
     7921 cggtgtacag cacgctgggc gcgcgcctgg acgtggtgga aatgctcgac ggcctgatgc
     7981 agggtgccga ccgcgacctc gtcaagatct ggcagaagat gaacgccaag cgcttcgacc
     8041 acatcatgct caagaccaag acggtgggcg ccaaggcgac gcctgcgggc atcgaggtca
     8101 cgtttgcatc cgcggaggag ggcggcaccg cccccgcgcc gcaggtgtac gacctggtgc
     8161 tgcaggccgt gggccgcacg cccaacggca agaagatcgc cgccgacaag gccggcgtgg
     8221 ccgtgaccga gcgcggcttc atcaacgtgg acatccagat gcgcaccaac gtgccgcaca
     8281 tcttcgccat cggcgacatc gtcggccagc ccatgctggc gcacaaggcg gtgcacgagg
     8341 cgcatgtggc ggccgaggtc atcgccggtg aattgaaggg cgacgaagcc ctggcgcgcg
     8401 ccgccttcaa tgcccgggtc atccccagcg tggcctacac cgaccccgag gttgcctggg
     8461 tcggcctgac cgaagaccag gccaaggcgc agggcatcaa ggtgaaaaag ggcctgttcc
     8521 cctggagcgc ctccggccgg gccattgcca acggccgcga cgagggctac accaagctgc
     8581 tgttcgacga agccacgcac cgcatcgtcg gtggcggcat cgtcggcacg cacgccggcg
     8641 acatgatcgg cgaggtggcg ctggccatcg aaatgggcgc cgacgcggtg gacatcggca
     8701 agaccatcca cccgcacccc acgctgggcg agagcatcgg catggccgcc gaagtcgcgc
     8761 atggttcatg caccgacgtg ccgccgcaac gcaaatgaag ctattacata tttagctggc
     8821 cgcgcagtgc cagaaagggc tgcgcggctt ttctcccgca agagcggcgg ctggcgaaac
     8881 acgccgtgcc ggcgacggca tggcgcccgc ggaacgtcga tcccatgtca ggcggggcag
     8941 atctccttcc gtgcctgagc cgcatttgcg gcgctgagcg attcagtccg cgtggcttgg
     9001 cggtggggct agaacgtttg acccacatca ttcacgctct gggttgcagg ccagacgtcc
     9061 cacagccgcg tggccgaact cgtatccttg gtcaacgggc agagactgtg tcgcttcgcc
     9121 gcggaagtcg aagatcaccc gagcatgact tgacgccatg gcgggcgcgt tccaggttat
     9181 gctggcgcca ccctgttcga cgcaagaggc cgacatggca tggcacactt ggctgatcta
     9241 ttttgtagcg gcgctcggcc tgtcgctgtc gcctgggccc aacgggctgc tggcgctgac
     9301 gcacggtgcc ttgcatgggc gtcgtaagac gctgtttacc gtcgccggtg gcgcgctggg
     9361 ttttgccacc gtcatcgcct tgtcgatgtt cggcatcggg gccctattgc aggcctcgct
     9421 ggtctggctg accgtgctca agtgggtggg tggcggctat ctggtttggc tgggcgtgca
     9481 ggtgtggcgc gccccgccca ttgctgcgct ggccctgcaa ccgcagggtg aggtcaacgg
     9541 ctggtccttg tttcgacagg gcgcgctgtc ggcgctgacc aatcccaagg gcattctgtt
     9601 cttcgctgcg ttcctgccac agttcatcga tccggcacgc agcctgctgc tgcagtactt
     9661 catcatggcc ggcaccttcg ttgccatcga attcatcacc gaggtgctga tagcaagcat
     9721 ggcgcagcgc atcagcggct ggctggcgcg cgtgggacgc ggtttcaacc gcgtgtgtgg
     9781 cggcatcttt gctgcgatcg gggtggcgct gccgctgcgc gcctgagcgc ggtcgtggcg
     9841 gcgctgtgca cccgtcaggt gtgccgcagt ggtgctgccg ggtacgtggc gtgcgcaacc
     9901 accagctgaa gatcaacgaa gcaaccggac ccggtgttcc accgtcgctc accaagatgg
     9961 cgagcggtca attgccgttc ctgggggtca tgtggatggt ggcgtcatcg ccagatggcg
    10021 gtgacgctcc aaccattgca tcaacggcaa ggcgagcgcg attttctcct gggtgccgag
    10081 gctgctgttc cagatttcgc gcatgcatgc ggcatgcacc tcgaagtact gcggctgtga
    10141 aaaatcccag ctgcgcgaca ggctgcccgg tttgtagaac ttgtgacaca gcagctcggg
    10201 cacccgcacg aagctgccca gcagggccag atggaggacc cagggccagt cggccgagaa
    10261 ctcgcccgcg ccgtgcttct tcagtccgcc gatctggcga gccatcgaca gccggaacag
    10321 cccacggttg ggcacccacc acttgcctct gcgctccagc acgcgcacgc cgcggatgaa
    10381 cgccgatcgc tcgccatcca gcgtggggta gctccagaac tcctgacctc cctgggtgtc
    10441 ggtcagcagc acgtccgaga aggccacgca ggcctgagga tcggcatcca gctccgccgc
    10501 caggcgggca atgtaggtgg gcgccagcag gtcatcgtgg aaggcgaaca gcgcatagtc
    10561 gccctgcgcc tgcgacagca ggaagttgct gttgccgacc cagcccaggc gcgaggtctg
    10621 cctgacgacg cgaaaacggg ggtcgcgcag cgcatggcgc tcgcagatcg cctgggtgtc
    10681 gtcgtcgcaa aggtccaccg acaccagcac gtgtagcgca ggatgggtct gcgccgacag
    10741 ggcgtcgagc gtgggttgta tgaattcggc agcctggtag gcgggtacca gcgcggttac
    10801 ggccgatggt gagatcgtgc tcatggtgca gcggccccat cgggatcgac gtcggtccag
    10861 gtctgtgcca gcgccgtgcg ctggcccagc aacaggtcgg ccgcctgcac ggcgtaccag
    10921 ggggcggtcg tgagcttgcc gctgtccacg ctcagatagc gggcatcgcc gcgggggcca
    10981 ttgaaggacc gcgcatgcag accgctgtcg agccgatcca catcttcgcg cccataggcg
    11041 aagatggcgc ccgcgtccac gttcagcacg gtggcgcgat gcaggcccgg gtaccagcga
    11101 tcggccgcgc gcaggatcgc ctcggccagt gcctgggcct gatcgggcgg tacatcacca
    11161 cggcagggcc catcccactc gggcggcggt accagggctt cactccagcc gcgcaggcct
    11221 tccgggtacc aggagacgaa ggccgtgcca ttggggcgca tcaccacgtc gccatagcgg
    11281 cccagcacca tcgtgaccga actgaggtgc tcccagcctg caggcagccg gacgatgacc
    11341 cggtacttga gtcggtgcag ccagccgggt gccggctgca gtcccatgct ggcatccagc
    11401 cccaggcgct gctcccaggt ggcgttcacc acctgccgtg cacaggcctc cagcggctcc
    11461 agcggttcgc cgccggaccc cagcacctgg ccgtggaggg tgacaccgtc cggagccggt
    11521 gcgaaccggg tgatgcgccg cccaggcagc cagtcgatga gtggatgggt gatcaccgca
    11581 cgccgcagcg cagcggccag cgcgtcggtg tcgatcgcgc gctcggcggt ggtgaacacc
    11641 gattcgccgg gagcgagcac caggccctgg tgctcgtccc gagatgccgg ccaggccagc
    11701 cgcgtggggc gctggcccag gtagctggcc tcggggtgct gggtcatcca atgcttgtac
    11761 agcgcctgca cgcgatcaaa gtgacgcgcc agggtgggcg ccggcaggac cgaatcgcga
    11821 gccacgaggt aggcgaacgg ggtggacagc ggcaaggccg cggccaccgg ccgacccagc
    11881 cagcgctcca gcaggtgctg aaacaccagt gcgccgcgta actgcacgcg ggccgtcgcg
    11941 ccgctggcgt cggcagcata gatcagaccc agatgaatct tgccttcgtt gcgcaggctg
    12001 gcgcggttca tgggcagcgc atcctgatcc agcagcacca cgggctgacc tcggtgcgca
    12061 agctccagcg cgatggcgca gccttgcagg ccggcgccaa ccacccacac gcgggaatcg
    12121 ggaacgctgc ggctcatggc tcgtgcaaga gtgaccgctc atcgcccggc gttggtcgtg
    12181 gtgcgcgcct gccctggcaa ttgccatgcg cctgcatcca cgtcgcgagc atcgctggtc
    12241 ccgtcatagg cgtgccaggc gagcaagtgc gttgtcgagc gtgccgtcct gtttggcgaa
    12301 gcaaaaacga atcaccttct ggtcgaagcc atcaccatag aacgccgaca gcggaatcgc
    12361 tgccacgccg atctcgcgtg tcagccactg gcagaagtcg gcttcggaca ggtcacgctc
    12421 gggcaccgag agttcatcga tcgccgcgca ctggaaatac gtgccttcgc aaggcagcag
    12481 ccgaaagcgc gtctgcgcca ggccggcgcg aaacaggtcg cgcttggcgg cgtagaagga
    12541 gggcagttcc aggtagggtc gggggtcctg catgtacgcg gcaatgccgt actgcatcgg
    12601 tgtgttgaca gtgaacacgt tgaactggtg caccttgcgg aactccgccg tcagtatggc
    12661 cggtgcagct acgtagccga ctttccagcc ggtgacgtga caggttttgc caaagctgct
    12721 gacgatgaag ctgcgcgccg ccaggccggg gtagcgcgcc gcgctttcgt ggcgcagtgg
    12781 ggcgtagacc atgtgctcgt acacctcgtc gctgatcacc agcgcgtcgg tgggcgccag
    12841 cagctcctgc aggcgcagca tgtcctgcgc tgaccagacc atgccgctgg ggttgtgcgg
    12901 gctgttgatc aggatggccc gggtgcgcgg cgacagagcg gcggcgaggc ggtcgaagtc
    12961 cggccgaaag ctgccgggcg tcagcggcac gcgcaccacg cgggcgccgc tgagttcgat
    13021 gttcggcacg tagctgtcgt agcagggctc cagcacgatc acttcgtcgc ccgggccggc
    13081 gcagcacagg atggcggtca ggatggcctg ggtggcgccg gcggtgacgg tgatttcggc
    13141 gccggcatcg tagtggcggc cgtacagcgc ctcgatcttg gctgcgatgg cctcgcgcag
    13201 cgccggcaca ccgggcatcg gcgggtattg gttcaaaccc tgccgcatgg ccgccgtcac
    13261 ggcgtcgacc agcgccgggt cgcagtcgaa gtccggaaaa ccctggccca ggttgacggc
    13321 ctggtgctgc gtcgccagcg ccgacatgac ggtgaagatg gtggtgccga cgttcggcag
    13381 gcgcgaaggg acggccggcg tgcgctcaga gttcatagtc gttcaggtgg ccgctcatgg
    13441 ccttgagaat gaggtccttg tccagccggt cggacagcag gctggcaaaa tggtagacga
    13501 agtcgcgcag ccaggcgccg cgcttgagcg cgatgcgcgc cacgttctgt cccagcagcg
    13561 gacccagcgg tcgcgccacc aggtcgccct ggcgatcgtc ggccatggcc atctcggcga
    13621 cgatgcctat gcccaggccc agccgcacgt aggtcttgat gacgtccgaa tcgatcgcct
    13681 ccagcaccac ccttggcgtc agctggcgct gggcgaacgc ggcatcgacc cgggtgcgcc
    13741 cggtgaacga ggggtggtag gtgatcaggg gctgtgccgc aatgtcctcc agcgccaggc
    13801 gctctttttt tgagagcgga tgggtggccg gcaggaccag cacatgctgc cactcgtagc
    13861 agggcagggt gaccagttcg gggtagctgg ccagcgactc ggtggccacg ccgatctcgg
    13921 ccgtctcctc gatcaccatg cgcgccacct gatcgggcgc gccctggtgc aggctgatgt
//
LOCUS       DAOOOG010000052        13785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4833_length_13785_cov_26.631173, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000052 DAOOOG010000000
VERSION     DAOOOG010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13785
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4833_length_13785_cov_26.631173"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="PK242_05720"
     CDS             <1..1244
                     /locus_tag="PK242_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOZ93549.1"
                     /translation="RDTPIISFVNKMDREVRDPLDILDEVERELGMPCCPMTWPVGQG
                     KTFGGIIDLRTQSMTVFQAGSEKRPEDFEVIPLTEVDKLRARFGKAYDDAIESMELAV
                     GASIAWDHQEFLAARLTPVFFGSGVNNFGVQEVLNAVVDMSPPPGPRMAWREVGRERE
                     QVTIRPEDKGFAGVVFKVQANMDANHRDRIAFVRVASGRYTPGMKMKVQRTGKELRPT
                     SVVTFMSQRREAVDEAYAGDIIGFTIHGGVQLGDSITDGPALQFTGLPFFAPELFMTV
                     VLKNPLRSKQLQQGLMELGEEGAIQVFKPDAGGNMLLGAVGQLQFEVVQHRLKTEYDC
                     DVRLEGCQYTGARWITADTPAELREFENAYPLRMAHDAAGTLTYLCTSPYDVRLAQER
                     FPKIHFHPLREHAGLELQARS"
     gene            1241..2644
                     /locus_tag="PK242_05725"
     CDS             1241..2644
                     /locus_tag="PK242_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93550.1"
                     /translation="MSVTPQCPPDAATRRRAVRIHLCTAAILLAVYLGWYAGVWPGPM
                     GQDGYSLVANINQGGARYTDKDPAWLLYALGTYGLSHRIEVLVLPLVLLQVLILTRLI
                     GWLYVQRQPLAATGLLVLVACAPHVLNFATSLYPDAIFSLAFVGLLFEIWLALRRGHL
                     NGWTIAALFVLFPAACYFKGNGIVMLVPVAYLAMRLKGGSRWLVVAIALSWTLAIQWG
                     GKVADLGRGHGALKPLILFETVNFMQTRPMNLWETRHMVTDRTREIMARHISQEDIDR
                     LYDRDYWDTLWHQNQDRVRFRHMSKTDFRHLRSDFLTYNLWRNLPAFTASRVNIFLAS
                     ALAQGGMVGPDNARHGLRAVDTLSVYNPFGFTRLPDTLNQLFHASYNWRFLLWSPFVG
                     VALIGLCLRRSLRHRDRDALVISGTLLVQLLGVFLFSIAAEYRYLLIFFYAPLLLLPM
                     VLVRAAGQTPPRAQAAA"
     gene            2699..3046
                     /locus_tag="PK242_05730"
     CDS             2699..3046
                     /locus_tag="PK242_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93551.1"
                     /translation="MSSLTLLATLLCVFCIAVGQLLFKKAALALPPHPQLIDWLTNGW
                     LIASLALYGLTTLGWVWILRHAPLHLAYPFMGLAFLIVPTLAWAFLGEPLHWRTLAGG
                     VLIMAGVALASNS"
     gene            3057..4016
                     /locus_tag="PK242_05735"
     CDS             3057..4016
                     /locus_tag="PK242_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ93552.1"
                     /translation="MRIAVAIPCYKVTQHVLGVIAAIGPEVEAIYAVDDACPEGSGRF
                     IEQNCRDARVRVLYHPENRGVGGAVMTAYQAALADGVDIVVKIDGDGQMDPALIPKFV
                     RPILRGRADYTKGNRFYRPESLRGMPRIRLFGNAALSFVTKLSSGYWPIMDPTNGYTA
                     IHAAALRRLPLEKLERRYFFESDMLYRLNTIRAVVQDVPMDAVYADERSNLRVSSVLP
                     EFMRKHVQRFFKRYVYVYLVRDFNLGSVYSLAGVLLSVLGLGFGVAQWVHSVTTGRPA
                     SSGTVMLAALPLMIGIQFLIAFLHHDVDNVPREPLSADDRDPE"
     gene            4013..4846
                     /locus_tag="PK242_05740"
     CDS             4013..4846
                     /locus_tag="PK242_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOZ93553.1"
                     /translation="MTPLSALPGTAWSAVHGVFTDIDDTLTTDGAITPDALQALGELK
                     AAGLRLIAITGRPVGWSEPFAATWPVDAIVAENGAVALIPDQIGLQRAPGVRRQLSKI
                     YQQDATLRAAQYQRMQQVLAHIEATVPGARRATDSPGRETDIAIDHSEFCHLPQAAIE
                     RCVQLMRAAGMNATVSSIHINGWFGPHNKLEGARWIVRRLLGRDLDAELARWIYVGDS
                     TNDQLMFEHFPHSVGVANIARFVPQLTHLPRYVTAAERGAGFAELAARLCAQRRGSPD
                     H"
     gene            5027..9028
                     /gene="putA"
                     /locus_tag="PK242_05745"
     CDS             5027..9028
                     /gene="putA"
                     /locus_tag="PK242_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HOZ93554.1"
                     /translation="MSTTIGIKVDDLLRERIRNAAQNLNRTPHWLIKQAVVQYVDALE
                     RGATTIRVLGADEATPEDGREEAQPAAPQEAQPFLAFAQSILPQTPLRAAITAAWHRP
                     ETECLPALLPLARTQDEAQAAKVRELATRLVQGLRDAPVTSGVAALVQEFSLSSQEGV
                     ALMCLAEALLRIPDKATRDALIRDKISKGDWKSHVGRSPSLFVNAAAWGLVLTGKLTS
                     TNSEKSLSSALTRVIGKGGEPLIRQGVHRAMKLMGEQFVTGQNISEALANSRKYEAQG
                     FRYSYDMLGEAAATEADAQRYLRDYEQAIHAIGAASAGRGIFEGPGISIKLSALHPRY
                     SRAQYDRVMQELLPRVLHLAELAKQYDIGMNIDAEEADRLELSLDLMEALCAAPSLRG
                     WSGIGFVVQAYQKRCPHVIEYLIDLARRSRRRLMIRLVKGAYWDSEIKRAQLDGLAGY
                     PVYTRKVYTDVSYLACARKLLEAPDAVYPQFATHNAQTLASIYQLAASVGGSYYSGQY
                     EFQCLHGMGEPLYQQVTGPASEGKLARPCRIYAPVGSHETLLAYLVRRLLENGANTSF
                     VNRIGDASVPVAELVSDPVEDALAIAREEGRLGAPHPRIALPHDLFAGEGRLARANSQ
                     GLNLAHEQQLASLAAALLYSTRQTYLAAAPQTPLPANPAQTPGWEPLRNPAELSDIVG
                     WVREASADEADAAAERAAQAAPIWAGTPPAARADALARAADLLEQRSQPLMGLIMREA
                     GKTLPNAVAEIREAVDFLRYYGAQVAAQFDNAAQRPLGVILAISPWNFPLAIFCGQVA
                     AALAAGNTVLAKPAEQTPLTAAAMVQILHEAGVPQGALQLVPGRGETVGAALVAHPQV
                     AGVMFTGSTEVARLIARQLSTRLSPNGHPIPLIAETGGQNAMIVDSSALAEQVVADVL
                     SSAFDSAGQRCSALRVLCLQEDVADRTLAMLHGALQEWTMGNPDRQSTDVGPVIDEQA
                     RAQIEAHIKRMEDAGQKVTRVQRQDGQLHGHFVAPAIIEIDSTSRLTREVFGPVLHVI
                     RFQREQLDALIDGINATGYGLTFGVHSRIDETIAHLSERVQAGNLYVNRNVIGAVVGV
                     QPFGGMGLSGTGPKAGGPLYLHRLAQGAPNTALAALPRAEADQAQQPTLQLLARLQQT
                     TLPGVDAALARAACDAALAASRQGASWLLPGPTGESNRYRLLPRGPVWALPRTPLGLV
                     HQLAAALASGNPCHVVLPQGDQGCAAAWDAIAAALGAAGQDLLRRADAASLQDASTPV
                     TALLFEGDGDALLQVGALVAAREGAIVRVESRSSDELAAGLGYDLAALVHEQSISTNT
                     AAAGGNAQLMTMA"
     gene            complement(9062..9466)
                     /locus_tag="PK242_05750"
     CDS             complement(9062..9466)
                     /locus_tag="PK242_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93555.1"
                     /translation="MALAAEAAAASPQVVDTLGGRMHVRWDEGAAATPHGQLVFFAQF
                     LAATGVFERWVQGCPLAYHSGNAPDKRDVLGTLMLGLLIANIGAALLHIKAAAEQFKD
                     LDRWDCLLRYISDRIAPVIGPPKPPLRQPAPG"
     gene            9619..9711
                     /locus_tag="PK242_05755"
                     /pseudo
     CDS             9619..9711
                     /locus_tag="PK242_05755"
                     /inference="COORDINATES: protein motif:HMM:NF026200.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9880..10785)
                     /locus_tag="PK242_05760"
     CDS             complement(9880..10785)
                     /locus_tag="PK242_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ93556.1"
                     /translation="MSDRVHQFRIFMQILDTGSFVGAARALKIPPATVSVAIRMLESE
                     LGVRLLHRTTRQVGLTHDGQRFLPMARMIVGDLDEIYHLFTNDEEVVAGKLHIRVPNR
                     IVWRLIAPALPSFLQQHPELELRFSSSDRDPDPLGEDVDGVIRMGNVRDDSLVHKPLG
                     QLDRVNCASPRYLATHAALQHPDELVQHWAIGYLLPSEARRAVWHFTGASGAAQSIPV
                     RHRVAVDDMDSYLACAQAGIGLIQAPRIDARHLLASGELVEVLPDWPATPVAVTALYP
                     HRHHQSRRLAAFLDWFGTLLDRAAA"
     gene            10825..11016
                     /locus_tag="PK242_05765"
     CDS             10825..11016
                     /locus_tag="PK242_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93557.1"
                     /translation="MNERSVCAESMILCFGFCMIPLFFLIVGMMGVKVRIRMARSHGR
                     SFGCGRLPFMGGQRRSKVC"
     gene            11063..11896
                     /locus_tag="PK242_05770"
     CDS             11063..11896
                     /locus_tag="PK242_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HOZ93558.1"
                     /translation="MEENQSQSAHLLIIDDDLDQLRLLVAALRGTSHRVSVATQGDQG
                     YVRAMVLQPNLILLDVCMPGRNGISVARLLKINPSTQHIPIIFLSALVGAQERLAGLK
                     AGAVDYITKPFLIEEVLERIRIHLELARKNLHPNTHDFTTKGSNQDPAVFNRQPVTLI
                     FRKLAIEYIMAHINESSLKVSDVAAHVEISVSKLNAIFEAIDGSSVFEFIRKERMQRS
                     ALMLGQSTLTIADIALEVGYSNPANFTTEFRKFWGRTPMQLRNESKINPDAWQQRIES
                     R"
     gene            11910..13592
                     /locus_tag="PK242_05775"
     CDS             11910..13592
                     /locus_tag="PK242_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOZ93559.1"
                     /translation="MDLNLATAAFITALVEFALGVSMLILWLREGRRYLAYWSSGFIA
                     FGFGSLLISMRNEIPDFLSIIVANFGTTLSSALLYVGICLFFGRRHSWLPWVVLALEA
                     AWLAYYTYVAYDTSARVYVYSVSQALIVLVTLQALFMVSRERAGRSNPEVVIVTVLLL
                     AVHLARIAGTPFFPLPRDFMNSGNFQTLLSFGLLMVHASYALALGNMHASGLNAELSA
                     ALAEAKIKERQKIEVLSYIGHDLRAPLATINGYSELLLADVSERGHRLLLSIQRIVKY
                     QVDLIDELLEYAKVELRPLAIRSTATDLPGLLRNISDYATTLCRQQNNQFIFQPSGRI
                     PRQIDIDGKRLQQVLLNLISNAAKFTHHGRVVLSIRAEPLKDISALYFSVSDTGVGID
                     LSRQTDIFSAFQDINAVKGGTGLGLFIAHRIVSAMGGSLRVASLPGQGSTFSFELSVP
                     VLEMSDFEWPAVAQQDGALVAPPTEFIFLEDALPDHQALDELADMASHGQFTDIECWI
                     ERHAQGAAHAAFAAHLYELLELFDFSAIRALALSCRNQKAAGRPNAQENSSK"
BASE COUNT         2346 a         4593 c         4417 g         2429 t
ORIGIN      
        1 agcgggacac acccatcatc agcttcgtca acaagatgga ccgcgaggtg cgcgacccgc
       61 tggacatcct cgacgaggtc gagcgcgagc tcggcatgcc ctgctgcccc atgacctggc
      121 ccgtgggcca gggcaagacc tttggcggca tcatcgacct gcgcacgcag agcatgacgg
      181 tgttccaggc cggcagcgaa aagcgcccgg aggacttcga ggtcatcccg ctcaccgagg
      241 tggacaagct gcgcgcacgc ttcggcaagg cctacgacga cgccatcgag agcatggagc
      301 tggccgtggg cgcgtcgatt gcctgggacc accaggagtt tctggccgcg cgcctgaccc
      361 cggtgttctt cggctcgggc gtgaacaact tcggtgtgca ggaggtgctc aacgccgtgg
      421 tcgacatgtc gcccccgccc ggcccgcgca tggcctggcg cgaagtgggc cgcgagcgcg
      481 agcaggtcac catccgcccc gaggacaagg gctttgccgg cgtggtcttc aaggtgcagg
      541 ccaacatgga cgccaaccac cgcgaccgca tcgccttcgt gcgcgtggcc tctggccgct
      601 acacgccagg catgaagatg aaggtgcagc gcacgggcaa ggaactgcgc ccgaccagcg
      661 tcgtcacctt catgagccag cggcgcgagg cggtcgacga agcctacgcg ggcgacatca
      721 tcggcttcac catccacggc ggcgtgcagc tgggcgactc catcaccgac ggcccggcgc
      781 tgcagttcac cggcctgccg ttcttcgcgc ccgagctgtt catgaccgtg gtgctcaaga
      841 acccgctgcg ctccaagcag ctgcagcagg gcctgatgga gctgggcgag gaaggcgcca
      901 tccaggtctt caagcccgac gccggcggca acatgctgct gggcgccgtg ggccagctgc
      961 agttcgaggt ggtgcaacac cgcctgaaga ccgaatacga ctgcgacgtg cgcctcgaag
     1021 gctgccagta cacgggcgcg cgctggatca cggccgacac gccggccgag ctgcgcgaat
     1081 tcgagaacgc ctacccgctg cgcatggcgc acgatgcggc gggcacactg acttatctgt
     1141 gcaccagccc gtacgacgtg cggctggcgc aggagcggtt tcccaagatt cactttcacc
     1201 cgctgaggga gcatgcgggg ttggaactgc aggccaggtc atgagcgtca cgccccagtg
     1261 cccacccgac gctgccacgc ggcggcgcgc ggtgcggatc catctctgca ccgccgcgat
     1321 cctgctggcg gtctacctgg ggtggtatgc gggtgtctgg cctggaccca tgggtcagga
     1381 tggttactcc ctggtggcca acatcaacca gggcggcgcc cgatacaccg acaaagatcc
     1441 ggcctggctg ctgtatgcgc tgggcaccta cggcctgagc caccggatcg aggtgctggt
     1501 gctgcctctg gtgttgctgc aggtgctcat tctgacccgc ctcatcggct ggctctacgt
     1561 tcagcgacag ccgctggcgg ccacggggct gctggtgctg gtcgcctgcg cgccacatgt
     1621 gctcaacttt gccaccagcc tgtatccgga tgccattttc agcctggcct tcgtggggct
     1681 gctgtttgaa atctggttgg cgttgcgtcg cggccacctc aacggctgga ccatcgcggc
     1741 cttgttcgtg ctgttcccgg cggcctgcta cttcaagggc aacgggatcg tcatgctggt
     1801 gccggtggcg tacctggcca tgcggctcaa aggcggcagc cgctggctgg tcgtggccat
     1861 tgcgctgtcc tggaccctgg ccatccaatg gggcggcaag gtggcggacc tggggcgcgg
     1921 ccacggcgcg ctcaaaccgc tcatcctgtt cgagacggtg aacttcatgc agacgcggcc
     1981 catgaacctg tgggagaccc ggcacatggt cacggatcgc accagggaga tcatggcccg
     2041 ccacatcagc caggaagaca tcgatcgcct ctacgaccgc gactactggg acacgctgtg
     2101 gcaccagaac caggatcgcg tccgcttccg ccacatgagc aagacggatt tccgccacct
     2161 gcgctcagac tttctgacct acaacctctg gcgcaacctg cctgcgttca cggccagccg
     2221 cgtcaacatt tttctggcct cggccctggc tcagggaggc atggtgggcc cggacaacgc
     2281 caggcacggc ctgcgcgcgg tggacaccct ctccgtctac aacccgttcg ggttcacacg
     2341 actgccggac acgctgaacc agctgttcca tgccagctac aactggcgct ttctgctctg
     2401 gagtccgttc gtcggtgtgg cgctcatcgg gctgtgtttg cgacgttcgc tgcgccatcg
     2461 ggaccgggac gcgctggtga tcagcggcac gctgctggtg cagctgctgg gggtgttcct
     2521 gttttcgatc gccgccgagt accgctacct gctgatcttc ttctatgcac cgctgctgct
     2581 gttgccgatg gttctggtgc gcgcagcagg tcagacgccg ccacgggcgc aggccgccgc
     2641 ctgagtttgg tgcgggcggg cctgcaatgc tagattcacc ctactttcca aggcattcat
     2701 gtccagtctg accctcctgg ccaccctgct gtgcgtgttc tgcatcgccg tcgggcagct
     2761 gctgttcaaa aaggccgcgc tggcgctgcc gccgcacccg cagctcatcg actggctaac
     2821 caatggctgg ctgatcgcct cgctggcgct ctatgggctg accaccctgg gctgggtctg
     2881 gatcctgcgc catgcgccgc tgcacctggc ctaccccttc atgggcctgg ccttcctgat
     2941 cgtgcccacc ctggcctggg cgttcctggg cgagccgctg cactggcgca cgctggccgg
     3001 cggcgtgctg atcatggccg gcgtggcgct ggcgtccaac tcctgaggcc ggcccgatgc
     3061 gcatcgccgt cgccattccc tgctacaagg tcacgcagca cgtgctgggc gtgatcgcgg
     3121 ccatcgggcc cgaggtcgag gccatctacg cggtggacga cgcctgcccc gagggcagcg
     3181 gccgcttcat cgaacagaac tgccgcgatg cgcgcgtgcg cgtgctctac caccccgaga
     3241 accgcggcgt gggcggtgcc gtgatgaccg cctaccaggc ggcactggcg gacggtgtgg
     3301 acatcgtggt caagatcgac ggcgacggcc agatggatcc ggcgctgatc cccaagttcg
     3361 tgcgccccat cctgcgcggg cgcgccgact acaccaaggg caaccgcttc taccgccccg
     3421 agtcgctgcg cggcatgccg cgcatccggt tgttcggcaa cgcggcgctg tcgttcgtca
     3481 ccaagctcag ttcgggctac tggccgatca tggacccgac caacggctac accgccatcc
     3541 atgccgcggc gctgcgtcgg ctgcccctcg aaaagctgga gcgtcgctac tttttcgaaa
     3601 gcgacatgct gtaccgcctg aacaccatcc gcgccgtggt gcaggacgtg ccgatggacg
     3661 ccgtgtacgc cgacgagcgg tccaacctgc gggtgtcgag cgtgctgccc gagttcatgc
     3721 gcaagcatgt gcagcgcttc ttcaagcgct atgtctacgt ctacctggtg cgcgacttca
     3781 acctgggcag cgtctacagc ctggccggcg tgctgctgag tgtgctgggg ctgggcttcg
     3841 gggtggcgca gtgggtgcac agcgtgacca cgggccggcc ggcgtcgagc ggcacggtga
     3901 tgctggcggc gctgccgctg atgatcggta tccagttcct gatcgccttt ctgcaccatg
     3961 acgtcgacaa cgtgccgcgc gagccgctgt cggccgacga ccgggatccc gagtgacgcc
     4021 gctgtccgcc ctgccgggca cggcgtggtc cgcggtgcac ggggtgttca ccgacatcga
     4081 cgacacgctg accaccgacg gcgccatcac gcccgacgcg ctgcaggcgc tgggcgagct
     4141 gaaggcggcc ggcctgcggc tgatcgccat caccggccgc ccggtgggct ggagcgaacc
     4201 cttcgccgcc acctggccgg tcgatgccat cgtggccgag aacggggcgg tggcactgat
     4261 tccagatcaa atcggcctcc agcgcgcgcc cggcgtgcgc cgacagctat caaaaatata
     4321 ccaacaggac gccaccctgc gtgccgcgca gtaccagcgc atgcaacagg tgctggccca
     4381 catcgaggcc acggtgcccg gcgcgcggcg cgccaccgat tcgcccgggc gcgagaccga
     4441 catcgccatc gaccacagcg agttctgcca cctgccgcag gcggcgatcg agcgctgcgt
     4501 gcagctcatg cgcgccgccg gcatgaacgc caccgtcagc agcatccaca tcaacggctg
     4561 gttcggcccg cacaacaagc tggagggcgc gcgctggatc gtgcgccgct tgctggggcg
     4621 cgatctggac gccgagctgg cgcgctggat ctatgtgggt gattcgacca acgaccagct
     4681 catgttcgag catttcccac acagcgtggg ggtggccaac atcgcgcgct tcgtgcccca
     4741 gctgacccac ttgccgcgct acgtgacggc ggccgagcgc ggcgccggct ttgccgagct
     4801 ggcggcgcgc ctgtgcgcgc agcggcgcgg gtcacccgac cactgacaag gctttcgggc
     4861 gatttggggg ctgccccacc gacctcgccg gccggacagg cacgatccga attgtggttg
     4921 caccaagggt taaccctgat atccgacagg tgcaacctat ggaacagttc cggctgcacc
     4981 ggaccaggca cacccggcca ccctctccac cgcccccgag aaacccatgt ctaccaccat
     5041 tggcatcaag gtcgatgacc tgctgcgcga gcgcattcgc aacgccgcgc agaacctcaa
     5101 ccgcacgccg cactggctga tcaagcaggc cgtcgtccaa tatgtggacg cgctcgagcg
     5161 tggcgccacc accatccgcg tcctcggcgc cgacgaggcc acgcccgagg acggccggga
     5221 ggaggcgcaa cccgccgccc cgcaggaggc gcaacccttc ctggccttcg cccagtccat
     5281 cctgccgcag acgccgctgc gcgccgccat cacggccgcc tggcatcggc ccgagaccga
     5341 atgcctgccc gcgctgctgc cgctggcacg cacccaggac gaggcccagg ccgccaaggt
     5401 gcgcgagctc gccacgcgcc tggtgcaggg cctgcgcgat gcgcccgtca ccagcggcgt
     5461 ggccgcgctg gtgcaggagt tctcgctctc cagccaggag ggcgtggcgc tgatgtgcct
     5521 ggccgaggcg ctgctgcgca tccccgacaa ggccacgcgc gacgcgctga tccgcgacaa
     5581 gatcagcaag ggcgactgga agtcgcatgt gggccgctcg ccgtcgctct tcgtcaacgc
     5641 cgccgcctgg ggcctggtgc tgacgggcaa gctgacttcc accaacagcg agaagagcct
     5701 gtcgtctgcg ctcacgcgcg tcatcggcaa gggcggcgag ccgctgatcc gccagggcgt
     5761 gcaccgcgcc atgaagctca tgggcgagca gttcgtcacc ggccagaaca tctccgaggc
     5821 cctggccaac agccgcaagt acgaggccca gggctttcgc tacagctacg acatgctggg
     5881 cgaggccgcc gcgacggaag ccgacgccca gcgctacctg cgcgactacg agcaggccat
     5941 ccacgccatc ggtgcggcat ccgccgggcg cggcatcttc gagggcccgg gcatctccat
     6001 caagctctcg gccctgcacc cgcgctattc gcgcgcgcag tacgaccgcg tgatgcagga
     6061 gctgctgccg cgcgtgctgc acctggcgga actggccaag cagtacgaca tcggcatgaa
     6121 catcgacgcc gaggaggccg accgcctgga gctgtcgctt gacctgatgg aggcgctgtg
     6181 cgccgcgcct tcgctccgcg gctggagcgg catagggttt gtggtgcagg cgtatcaaaa
     6241 gcgctgcccg catgtcatcg aatacctgat cgacctggcg cggcgcagcc gccgccgcct
     6301 catgatccgc ctggtcaagg gcgcgtactg ggacagcgag atcaagcgcg cccagctcga
     6361 tggcctggcc ggctacccgg tctacacgcg caaggtctat accgacgtga gctacctggc
     6421 ctgcgcgaga aagctcctgg aagcacccga cgccgtctac ccgcagttcg ccacacacaa
     6481 cgcccagacg ctggccagca tctaccagct ggccgccagc gtgggcggca gctactacag
     6541 cggccagtat gagtttcaat gcctgcacgg catgggcgag ccgctgtacc agcaggtcac
     6601 cggccccgca tcggaaggca agctcgcgcg cccctgccgc atctacgccc ccgtgggcag
     6661 ccacgagacg ctgctcgcct acctggtgcg gcgcctcttg gaaaacggcg ccaacacctc
     6721 gttcgtcaac cgcataggcg acgccagcgt gcccgtggcc gagctcgtga gcgacccggt
     6781 cgaggacgcg ctcgccatcg cccgcgagga gggccgcctg ggcgcaccgc acccgcgcat
     6841 tgccctgccc cacgacctgt tcgcgggcga gggccgtctg gcgcgcgcca actcccaggg
     6901 cctgaacctg gcgcacgagc agcagctcgc atcgctggcc gccgccctgc tctacagcac
     6961 gcgccagacc tatctggccg cggcgcccca gaccccgctg cccgccaacc cggcgcagac
     7021 gccgggctgg gagccgctgc gcaacccggc cgagctttcc gacatcgtgg gctgggtgcg
     7081 cgaggccagc gccgacgagg ccgatgccgc cgccgagcgc gccgcccagg ccgcgcccat
     7141 ctgggccggc acccccccgg ccgcgcgcgc cgacgcgctg gcccgcgccg ccgacctgct
     7201 ggagcagcgc agccagccgc tgatgggcct gatcatgcgc gaggccggca agaccctgcc
     7261 caacgccgtg gccgagatcc gcgaggccgt ggacttcctg cgctactacg gcgcccaggt
     7321 ggcggcgcag ttcgacaacg ccgcacagcg cccgctcggc gtcatcctcg ccatcagccc
     7381 ctggaacttc ccgctggcca tcttctgcgg ccaggtggcc gcggccctgg ccgcgggcaa
     7441 caccgttctg gccaagccgg ccgagcagac gccgctcacg gccgcggcca tggtgcagat
     7501 cctgcacgag gccggcgtgc cccagggcgc gctgcagctc gtgcccggcc gcggcgagac
     7561 cgtgggcgcg gccctggtgg cgcacccgca ggtcgcgggc gtgatgttca ccggctccac
     7621 cgaggtggcg cgcctgatcg cgcgccagct cagcacccgc ctgtcgccca acggccaccc
     7681 catcccgctg atcgccgaga ccggcgggca gaacgccatg atcgtggact cctcggccct
     7741 ggccgagcag gtggtggccg acgtgctgtc ctcggccttc gactcggccg gtcagcgctg
     7801 ctcggcgctg cgcgtgctat gcctgcagga ggacgtggcc gatcgcacct tggccatgct
     7861 gcacggcgcg ctgcaggaat ggaccatggg caaccccgac cgccagagca ccgacgtggg
     7921 ccccgtgatc gacgagcagg cgcgcgcgca gatcgaggcg catatcaagc gcatggagga
     7981 cgcgggccag aaggtcacgc gcgtgcagcg ccaggatggc cagctccacg gccatttcgt
     8041 ggctccggcc atcattgaaa ttgatagcac atcgcgcttg acccgtgagg tgtttggccc
     8101 ggtcttgcat gtcatccgat tccagcgcga gcagctcgac gccctgatcg acggcatcaa
     8161 cgccacgggc tatggcctga cttttggcgt gcacagccgc atcgacgaga ccattgccca
     8221 cctgagcgag cgcgtgcagg ccggcaacct ctatgtgaac cgcaacgtga tcggcgccgt
     8281 cgtcggcgtg cagccctttg gcggcatggg tctgtcgggc acgggcccca aggccggtgg
     8341 gccgctctac ctgcaccgcc tggcgcaggg cgcgcccaat accgccctgg cagcgctgcc
     8401 gcgtgccgag gccgatcagg cgcagcagcc cacgctgcaa ctgctcgcgc gcctacagca
     8461 gacgaccctg cccggcgtgg atgccgcgct ggcgcgcgcc gcctgtgatg cggcgctggc
     8521 cgcctcgcgc cagggcgcga gctggctgct gcccggcccc acgggtgaat ccaaccgcta
     8581 ccgcctgctg ccgcgcggcc ccgtgtgggc cctgccgcgc acgccgctgg gcctggtgca
     8641 ccagctggcc gcggcactgg ccagcggcaa cccttgccat gtggtgctgc cccagggcga
     8701 ccagggctgc gccgccgcct gggacgccat cgccgccgcc ctgggcgcgg ccggccagga
     8761 cttgctgcgc cgcgccgatg ccgcaagcct gcaggacgcc agcacaccgg tgacggccct
     8821 gctgttcgaa ggtgacggcg acgccctgct gcaggtgggc gccctggtcg ccgcgcgcga
     8881 gggcgccatc gtacgcgtgg aaagccgcag cagcgacgag ctcgccgcgg gcctgggcta
     8941 cgacctcgcg gcgctggtgc acgagcagtc catcagcacc aacaccgcgg ccgcgggcgg
     9001 caacgcccag ctcatgacca tggcgtgagg ggccgcgaga gactgatccc aaatccggca
     9061 gttaccctgg agccggctgc cgcaatggcg gcttgggtgg cccgatgaca ggggcgatcc
     9121 ggtcgctgat gtagcgcagc agacagtccc agcggtccaa gtccttgaac tgctccgcag
     9181 cagccttgat gtgcagcaaa gccgcgccga tgttggcgat cagcagcccg agcatcaacg
     9241 tgcccagcac gtcgcgtttg tccggggcgt tgccgctgtg gtaggccagc gggcagccct
     9301 gcacccaacg ctcgaacacg ccggtggcgg ccagaaactg cgcgaagaac accaactggc
     9361 catgcggcgt ggccgccgca ccctcgtccc agcgcacgtg catgcgcccg cccagcgtat
     9421 ccactacctg cggcgacgcc gccgcagcct cggccgccag ggccacttcg cgcttgcgtt
     9481 tgctctcacc cgtcgggtga gtgtgcccgc agcggacaaa gccccgccag tgcttgctca
     9541 gcgggcaatc acgagggatg aaccgccgga tgtaggctga ttcgcgacaa gatcagcaag
     9601 ggcgactgga agttgcatgt ggaccgctcg ccgtcgctgt tcgtcaacgc cgccgcctgg
     9661 ggcctggtgc tgacgggcaa gctcaccagt accagcagcg agaagagcct gtcctcggcg
     9721 ctcacgcgcg tcatcggcaa gggcggcgag ccgctgatcc gccaggcaga gcgtgcaccg
     9781 cgccatgaag ctcatgggcg aacaattcgt caccggccag aacgtctccg tcgcaaaggt
     9841 gcgccaggac aggggacggc gatggctgtg cgttctctct catgccgcgg cccgatccag
     9901 cagggtccca aaccagtcga gaaatgcggc cagtcgccgc gactggtggt gacggtgcgg
     9961 gtaaagggct gtgacggcga cgggagttgc gggccagtcc ggcagcactt ccaccagttc
    10021 gccgctggcg agcaggtgac gagcgtcgat gcgcggcgcc tggatcagcc cgatgccggc
    10081 ctgggcgcag gccagatagc tgtccatgtc gtcgaccgcg acacggtgtc gcaccgggat
    10141 ggactgtgcc gcgcccgatg cgccggtgaa atgccagacc gcccggcgtg cctcggaggg
    10201 cagcaaatag ccgatggccc aatgttggac cagctcgtcc gggtgttgca gggccgcatg
    10261 ggtcgccaaa tagcgggggc tggcgcagtt gactcgatcc agctggccca ggggtttgtg
    10321 caccagagag tcatcgcgca cattgcccat ccggatcacg ccatcgacgt cctcgccgag
    10381 cggatccggg tcgcggtcgg aactgctgaa acggagttcg agctcgggat gttgttgcag
    10441 aaacgaaggc agcgcgggcg caatcaggcg ccagacgatg cggttgggca cacgaatgtg
    10501 aagcttgccg gcgaccacct cctcgtcgtt ggtgaacaga tggtaaatct cgtccaggtc
    10561 gccgacgatc atgcgcgcca tgggcagaaa tcgctggccg tcgtgtgtca ggccaacctg
    10621 ccgggtggtg cggtgcagca agcgcacgcc cagctcgctt tccagcatcc gaatggccac
    10681 cgaaaccgtg gccggtggaa ttttcagggc gcgcgccgcg ccgacgaagc tgcccgtatc
    10741 gaggatctgc atgaaaatgc gaaactgatg gacccggtcg gacacagggg gcggcttgta
    10801 tgcaggcatc tttcataaac agcgatgaat gagcgctcag tttgtgcaga gtcgatgatt
    10861 ctttgttttg gtttttgtat gattcctttg ttttttctga tcgtcggcat gatgggcgtc
    10921 aaggtgcgca tccggatggc gcgatcccat gggcggtcct tcggctgcgg gcggctgcct
    10981 ttcatgggcg gccagcgtcg cagcaaggtg tgctgaattt tcaagtttga tccgatgttt
    11041 ttgaatggaa ttcggagaac ccatggaaga aaatcaatct cagtccgcgc atctgctcat
    11101 catcgatgac gatctggatc aacttcgtct gctggtcgcg gctttgcgcg gcacttcaca
    11161 ccgcgtcagc gtggcgaccc agggtgatca gggttacgtg cgggccatgg ttttgcagcc
    11221 caatttgatt ttgctggacg tgtgcatgcc gggacgcaac gggatttcgg tggcccgtct
    11281 attgaaaatc aacccctcca cccagcatat cccgatcatt tttctttctg cattggtcgg
    11341 cgcgcaagag cggttggctg gtttgaaagc aggagctgtc gattacatca ccaaaccatt
    11401 tttgatcgaa gaagtcctgg agcgaatacg cattcatctg gaactggcca gaaaaaactt
    11461 gcaccccaat acgcatgatt tcacgacgaa gggttcgaat caggatcccg ctgttttcaa
    11521 tcgccagcct gtgacgctga tttttagaaa attggcgata gaatacataa tggcgcatat
    11581 aaatgaatct tccctgaagg tttctgatgt tgccgctcat gtggaaattt cagtgtcgaa
    11641 actgaatgcc atattcgaag ccatcgatgg ctcttcggtg tttgaattca tccgcaagga
    11701 gcgcatgcag agatcggcct tgatgctggg ccagagcaca ctgaccatcg cagatattgc
    11761 cctggaagtc ggctattcga accccgcgaa ttttactacc gaattcagaa aattttgggg
    11821 cagaacgccc atgcagcttc gaaatgaatc aaaaatcaat ccggatgcat ggcagcaacg
    11881 cattgaatcc agatagatga tggtctgaaa tggatctgaa cctggctacc gctgcattca
    11941 tcaccgctct ggtggagttt gctctcgggg tgagcatgct gatattgtgg ctgagggagg
    12001 gcagacgata tctcgcctac tggagttctg gcttcatcgc cttcgggttc ggctctctgc
    12061 tcatttcgat gcgcaacgaa attcccgatt ttctttccat catcgttgcc aatttcggca
    12121 cgacactgag ttcggccctg ttgtacgtgg gtatctgcct gttctttggc cggcggcact
    12181 catggctgcc ctgggtggtg ctggccctgg aggcggcatg gctggcctac tacacctacg
    12241 ttgcgtacga cacttccgcg cgtgtgtacg tctacagcgt gtctcaggcg ctgatcgtgc
    12301 tggtcaccct ccaggccctg ttcatggtga gccgcgagcg tgcgggccga agcaacccgg
    12361 aagtcgtgat cgtgaccgtg ctgctcctgg cggtgcatct tgcgcgcatc gccggcacgc
    12421 cgtttttccc gctgccgcgg gacttcatga attcaggaaa tttccagact ttgctttcct
    12481 tcgggttgct gatggttcat gccagctatg cattggcgtt gggcaacatg cacgcatccg
    12541 gcttgaatgc ggaactgagc gcggcgcttg ctgaagcaaa aatcaaggag cgccagaaaa
    12601 tcgaagtcct gagctatatt ggtcacgact tgcgtgcgcc attggcgacc atcaatgggt
    12661 attccgagct attgctagcc gatgtgtctg agagagggca tcgactgttg ctttccatcc
    12721 agcgcattgt caaatatcaa gtggatttga tcgatgagtt gctggaatat gccaaagttg
    12781 agttgcgtcc gctggctatt cggtcaaccg ccaccgacct tcctggcttg ttgcgaaaca
    12841 tatccgatta tgcgaccacc ttgtgtcgcc aacaaaacaa ccagttcatc tttcagcctt
    12901 ccggcagaat tcctcgacaa atcgatatcg atggaaagcg tttgcagcag gtgctgctga
    12961 accttatttc caatgcggcc aagttcacgc accatggccg ggtggtgctg tcaatcaggg
    13021 ccgagccatt gaaagatatc tcggccctgt atttctccgt gagcgatacg ggggttggga
    13081 ttgatctgag ccgacagaca gatattttca gtgccttcca ggatataaac gctgtcaagg
    13141 gcggcactgg cttgggcttg ttcattgcgc atcgaattgt ttccgccatg gggggctcct
    13201 tgcgcgttgc cagtctgccg ggacagggaa gtactttttc attcgaactt tctgtccccg
    13261 ttctggagat gtccgacttt gaatggcctg ctgtggctca gcaggacggg gcgctcgtcg
    13321 cgccgcccac ggaatttatc tttctggaag acgccttgcc agatcatcaa gccctggatg
    13381 agctggctga tatggcttct cacggtcagt tcacagatat cgaatgctgg atcgagcgcc
    13441 atgcccaagg cgctgcgcat gccgcttttg ctgcgcacct gtacgaattg ctggaactgt
    13501 tcgatttttc ggcaatacgc gcgctggctt tgagctgtcg aaaccagaag gctgccggca
    13561 ggccgaatgc ccaggaaaac tcctcaaagt aaatcgccca tctgccagaa ccacgccaat
    13621 acccgcacac ggatgcccga tcgtgccgtc gcgcgtgctc acccttgtct cgttggccgg
    13681 atgtgcctga tcggatggag ggaatgaatt ggacatcgac gacactggcg attgtattga
    13741 atttctgaat ggtcaaaaca agatctcggt ttaattcgag atcaa
//
LOCUS       DAOOOG010000053        13765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4856_length_13765_cov_27.498687, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000053 DAOOOG010000000
VERSION     DAOOOG010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13765
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4856_length_13765_cov_27.498687"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PK242_05780"
     CDS             complement(<1..689)
                     /locus_tag="PK242_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HOZ93560.1"
                     /translation="MIDKIAASVAEALADVRDGATVLIGGFGTAGIPGELIDGLIAQG
                     ARELTVVNNNAGNGDRGLAALLAAGRVRKIICSFPRQADSQVFDGLYRAGRIELELVP
                     QGNLAERLRAAGAGIGAFFTPTGFGTELAKNPDGSPKETRRINGRDHVLEYPIHGDVA
                     LIRAERGDRWGNLSYRMAARNFGPVMAMAARCTVASVHEVVALGQLDPEAIVTPGIFV
                     HRVVRVPRVATA"
     gene            769..1059
                     /locus_tag="PK242_05785"
     CDS             769..1059
                     /locus_tag="PK242_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HOZ93561.1"
                     /translation="MSQISITRAHGLSPKKARLAAEAVAADLQAEYGLQYAWDDDGTL
                     SFQRAGISGQLTLERRQVTVQVRLGLLFLPFKASFEREIHDYFDQRFAPRSA"
     gene            1088..2542
                     /locus_tag="PK242_05790"
     CDS             1088..2542
                     /locus_tag="PK242_05790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOZ93562.1"
                     /translation="MTAQEKMSPVDTAWLRMDRPNNLMQILGVMLFDGKLDFARLRQR
                     IEQRLLSHARFRQFAREDATGWSWVDDPEFDLDNHLRRAALPGKGDKAELERFVADLA
                     STPLPPARPLWQIHVVDTHMGGQALVTRFHHCIADGIALVAVIMEMADDAPAPLPAAA
                     ATPPHHSHDDALWGMVWHPISRAVQASVRASTSLWAMSQDWLRNPWQALDYARLGSGM
                     TAEAAKLLVMPNDSTTRFKGTPGLVKRVAWSEPIDLREIKAVGKVLGCSVNDLLLSAV
                     AGALGAYLTDCGDATEGVELRALVPVNLRQAADAGKLGNRFGMVTLELPVGVANPLAR
                     LYETRRRMQAMKQSYQPALTLTLLGAAGMGPKLVQEQLLDFLANKATAVMTNVPGAQQ
                     PLYFAGARLRQPFFWVPQSGNIGMGVSILSYANQVQFGLITDRRLVPDPERIVSRFAE
                     EFDKLLMLVLLEPPECLHDPELVEAHNAFYWSSE"
     gene            complement(2638..3423)
                     /locus_tag="PK242_05795"
     CDS             complement(2638..3423)
                     /locus_tag="PK242_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93563.1"
                     /translation="MSKTAWKAFPHPDPAYTYAGTALKKHWARLHQGDAEPWPTDSAA
                     QAAWRAYHAGEFAQAVALGLKAGNASGTNAANKAAMIHAASVEDDQARKLALLQEVAR
                     RCEALQTSQPGNVNAWYYHGYALGRYSQGISVAKALAEGLGSKVRESLQKAIDLEPRH
                     ADAHIALGTWHAEIINKVGAVVGGLTYGAKKDAAEKHFKTALALNPESPIAMTEYANG
                     LAMMFGKSRLKEAEQLYARAAQCTPADAMERLDVEVARAEVGG"
     gene            complement(3420..4076)
                     /locus_tag="PK242_05800"
     CDS             complement(3420..4076)
                     /locus_tag="PK242_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ93564.1"
                     /translation="MNKPKRRTPERILETALRLFNEFGEPNVTTTVIADEMNISPGNL
                     YYHYRNKDEIVNALFARYEREISELLTLRDTAPREVEDVWFFLHLLFETISTYRFIYR
                     DINDLLTRNRLVETHFQRILAHKQQTAVALCHGLAAAGALDATPDEIAALAVNITLVT
                     TFWLSFEHARHPRRPPDIGRGAHQVLSLTAPWLREPARRLLEKLSLSYVHSTSRGDAR
                     "
     gene            complement(4129..5304)
                     /locus_tag="PK242_05805"
     CDS             complement(4129..5304)
                     /locus_tag="PK242_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOZ93565.1"
                     /translation="MSKIGLALAGGGPLGGIYEVGACVALEEAIDGLSLVDADVYVGV
                     SAGSFVAASLANGFEPRRLARMLLGSDGSQAFDPSLLLRPALREYLNRARAIPRLALQ
                     AAREYLGNPWRHGLFESFQRLSRAIPTGLFDGAGVDAVLRRQFSEVGRSNDFRRLKAR
                     LYVVATDLDSGQAVAFGGPGRDDVPISTAVQASAALPGLFPPVEIQGRHYVDGALIKT
                     LHASVALRDGVDLLLCVNPLVPFDAQLAEQHGAGHRQLSEGGLPVVLAQTFRAIIRSR
                     MRVGMDRYQHQFKHADVLLFEPAQDDEAMFFANLFSYRDRARLCEHAYQRTREHLYQR
                     RDELAPVLARHGLQLNLEVLRDRTRTLLGAEPAATATQGLARTLDQLQHWIDARRAA"
     gene            complement(5474..5950)
                     /locus_tag="PK242_05810"
     CDS             complement(5474..5950)
                     /locus_tag="PK242_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOZ93566.1"
                     /translation="MASKLKTISDNNQLAATIKESTQQIWLAGLGAFAKAQEEGGKMF
                     DALVKEGEMMQKKTREVADARIAAVAGRAADTWDRLEQVFEDRVARALGSLGVPSKKE
                     VDVLSKRIAELTAAVQELAQNKGKAAPRLPAAKPAVAKPAPAKKAAPAKKAARKSA"
     gene            6133..8112
                     /locus_tag="PK242_05815"
     CDS             6133..8112
                     /locus_tag="PK242_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ93567.1"
                     /translation="MQYFITGATGFIGKRLVARLLEREGARIFFLVRQRDPERLQALY
                     DFWGCDDTRATPVVGDLTQDMLGVSAADRRKLGKKTTHFFHVAAVYDLSADAERQLQV
                     NVEGTRQAVRFAEAIGAKHFHLFSSIAAAGLYEGVFREDMFEQAEGLDHPYFRTKHDS
                     EGIVRNECRIPWRVYRPAIVVGDSRTGEMDKIDGPYYFFKLIQKLRRLLPAWMPAIGI
                     EGGRINLVPVDYVVDAVDHIAHLKGEDGRCFHLVDPRPRRVGDVLNIFARAGHAPQMS
                     VRVNAALLGFIPKYMRKALLALTPVRRVRDVLMKELGLPDDILSFVNYPTRFDSRETE
                     RVLKGTRIAVPPLEDYAWRLWDYWERCLDPDLFIDRSLGGRVKGQVVVVTGASSGIGK
                     AVALRLAEAGAIVVTMARGKPALDAAASEFEALGLKLHTYEADLADLAQVDAVAQQIL
                     HDHGPVAILINNAGRSIRRAIENSYERFHDFERTMQLNYFGALRLNLNLLPAMAERRT
                     GHIINISSIGVLTNAPRFSAYVASKAALDAWTRCAASEFADRGIHFTTINMPLVKTPM
                     IAPTALYDQAPTLTPEEAADMVCEAIVHRPTRMATRLGITGEVLHALMPRVAQAVMNT
                     SFRMFGESQAAASGEKTAPTRDQLALQNWMRGIHF"
     gene            complement(8196..9071)
                     /locus_tag="PK242_05820"
     CDS             complement(8196..9071)
                     /locus_tag="PK242_05820"
                     /inference="COORDINATES: protein motif:HMM:NF018525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOZ93568.1"
                     /translation="MLSALLLWGMAVELLVWGAIGRAGLGWSWGATLALALGALLLGR
                     ALLLAVTWLVAWRHRSAAPPLSAGAWLVMLWREYWAFVGGFVLFQPWARLAMPADRLR
                     PSAQPILLVHGYGCNRAIWWWLRPRLEAAGHVVATLSLEPPWGDIDGFAEQLQQRIEA
                     VCAATGAPRLTLVAHSMGGLVSRACLARHGAARVAALISIASPHGGSRLAHLGLGRCA
                     RQMQPGSHWLARLAQQRVNVPFVSIRTPQDNFVMPQQLQRHADARDEPLPGVGHMAAL
                     CDERTLRLVLAHTKL"
     gene            9262..9987
                     /locus_tag="PK242_05825"
     CDS             9262..9987
                     /locus_tag="PK242_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93569.1"
                     /translation="MSETTDTVSERQACTHALTHWLTEARHQRQRAAAEPRRAARRQA
                     LRAFQAARLARTHADLLDSPRYGRAAAFFLTDLYSPKDLGPRDAEIERVLPLITSTLP
                     TAGLHALLQAAELDALSERFDAAMVHALGERLDTSLDDADYAAAYRTVDDAPGRQHQI
                     ALILGTGQTLDRLAHKPGLAALLKMMRGPAQLAGLGELQSFLERGFTAFRSMRSAQAF
                     LDTIIERERALSARLFGGGQSLA"
     gene            10008..10448
                     /locus_tag="PK242_05830"
     CDS             10008..10448
                     /locus_tag="PK242_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HOZ93570.1"
                     /translation="MFAPSQADVRRFFCHAWAKARGAQPMDAIEQLAAGWIAEHPEWH
                     ADFADAEAAVARHYGDGAVGGNPFLHLSMHLSISEQCSIDQPRGIRQAVELLAHRRGD
                     LHAAHHEVMECLGQMLADAQSRRAAPDGDGYVACVQRRATRDGA"
     gene            complement(10738..11382)
                     /locus_tag="PK242_05835"
     CDS             complement(10738..11382)
                     /locus_tag="PK242_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ93571.1"
                     /translation="MFAALGTALAMTLPVQAQVQGNATAGEGKVAMCIGCHGIAGYKS
                     SFPEVYRVPKIAGQSAKYIGTVLEEYRKGTRKHPSMRGIAGSMSDQDIADVAAYYEAL
                     GKVEGATELPAPREPSAQVAQLLQKGACVSCHGDNYSKPIDPSYPKIAGQYPDYLYAA
                     LRAYRDDSHATWGRGNPVMAGMAKQFSDPELKAMARYIGSLPGEVRTVPQSRFR"
     gene            11512..12354
                     /locus_tag="PK242_05840"
     CDS             11512..12354
                     /locus_tag="PK242_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOZ93572.1"
                     /translation="MKFQGTDSYVATQDLMLAVNASITLKRPLLVKGEPGTGKTMLAE
                     EVAQALGLPLLQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDDRVKDIHNYIVKGVLWQ
                     AFTADEPVALLIDEIDKADIEFPNDLLRELDRMEFHCYETRETIRARHRPVVFITSNN
                     EKELPDAFLRRCFFHYIKFPDADTMRQIVDVHFPDLKKELLAAAMKVFFDVRGLPGLK
                     KKPSTSELLDWLKLLVAEDIPAEALQSQDDKVAVPPLVGALLKNEQDVTLFEKLVFMN
                     QRHR"
     gene            12374..12598
                     /locus_tag="PK242_05845"
     CDS             12374..12598
                     /locus_tag="PK242_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93573.1"
                     /translation="MHPLLVEGLSDAVGFVGGALAGFWLARLLGFDVFSEGYDSASVL
                     AIAAVGLGGGLGLGAARRWRRARQAERKER"
     gene            12602..13204
                     /locus_tag="PK242_05850"
     CDS             12602..13204
                     /locus_tag="PK242_05850"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOZ93574.1"
                     /translation="MAFVEPVTLRGHGLRLEPLALTHEAGLAAAAADGALWRIRVTSV
                     PEPQETRAYIETALQMREAGSRLAFAVLDDASGRMLGSSSYHDILPAVRRVEIGYTWY
                     ARSVQRTHVNTAAKLLLMGHAFDTLGCHVVGWRTDNFNHASQRAIERLGARKDGVIRG
                     HALRRDGTIRDTVMYSMRSGEWPEARAQLLYLLELRAQAR"
     gene            13223..>13765
                     /locus_tag="PK242_05855"
     CDS             13223..>13765
                     /locus_tag="PK242_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93575.1"
                     /translation="MLIDFFYALRAAKLPVSVKEFLTLLEALQANVVGPRQPDACTMD
                     DFYYLARTALVKDEKHFDKFDRAFAAYFKGVEMLTDFSKEIPADWLRKMLERELTPEQ
                     KAAIEKMGWDELMETLRKRLEEQKGRHEGGSKWIGTGGTSPFGHGGYNPQGVRIGGKG
                     GNRSAVKVWDQRAYRDYDDQQ"
BASE COUNT         2135 a         4868 c         4661 g         2101 t
ORIGIN      
        1 gcggtcgcca cgcgcggcac gcgcaccacc cggtgcacga agatgccggg cgtgacgatg
       61 gcctcggggt cgagctggcc cagcgccacc acctcgtgca cgctggctac ggtgcagcgc
      121 gcggccatcg ccatcaccgg gccgaagttg cgcgccgcca tgcggtagct caggttgccc
      181 cagcggtcgc cgcgctcggc gcggataagc gccacgtcgc catggatcgg gtattcgagc
      241 acgtggtcgc ggccattgat gcggcgcgtt tccttggggc tgccatcggg attcttggcc
      301 agctcggtgc cgaagccggt gggcgtgaag aaggcgccga tgccggcccc ggcggcacgc
      361 aggcgctcgg ccaggttgcc ctgcggcacc agctccagct cgatgcggcc ggcgcggtac
      421 aggccgtcga acacctggct gtcggcctgg cgcggaaagc tgcagatgat cttgcgcacg
      481 cgcccggcgg ccagcagcgc ggccaggccg cggtcgccgt tgccggcgtt gttgttgacg
      541 accgtcagct cgcgcgcgcc ctgcgcgatc aggccgtcga tcagctcgcc cggaatgccg
      601 gcggtgccaa agccgccgat cagcaccgtg gcgccgtcgc gcacatcggc cagggcctcg
      661 gccacggaag ccgcaatctt gtcgatcatg aaggggttcg ctccaatgcc tggtgccagg
      721 acggcgcacc catggccgcc gccgccccgc gcactttaca ctttgcgcat gagccagatc
      781 agcatcaccc gcgcccatgg gctgagcccc aagaaggccc gcctggccgc cgaggcggtg
      841 gccgccgacc tgcaggccga atacggcctg cagtacgcct gggacgacga cggcacgctg
      901 tcgtttcagc gcgcgggcat cagcggccag ctcacgctgg agcgccgaca ggtgacggtg
      961 caggtccggc tggggctgct gtttctgccg ttcaaagcca gcttcgagcg cgaaattcac
     1021 gattacttcg accagcggtt tgccccgcgc agcgcctgaa cccaccaccc tgcccttgcc
     1081 aatcaccatg accgcccaag aaaagatgtc gcccgtcgat accgcctggc tgcggatgga
     1141 ccggcccaac aacctgatgc agatcctggg cgtgatgctg ttcgacggca agctggactt
     1201 cgcccgcctg cgccagcgca tcgagcagcg cctgctgtcg cacgcgcgct ttcgccagtt
     1261 cgcgcgcgag gacgccaccg gctggtcctg ggtggacgac cccgagttcg acctcgacaa
     1321 ccacctgcgc cgcgcggcgc tgccgggcaa gggcgacaag gccgaactcg agcgcttcgt
     1381 cgccgacctg gccagcacgc cgctgccgcc ggcgcgcccg ctgtggcaga tccacgtggt
     1441 cgacacccac atgggcgggc aggcgctggt gacgcgcttt caccactgca tcgccgacgg
     1501 catcgcgctg gtggcggtga tcatggaaat ggccgacgac gcccccgcgc cgctgccggc
     1561 ggcggccgcc acgccgccac accacagcca cgacgacgcg ctgtggggca tggtctggca
     1621 cccgatctcg cgcgccgtgc aggcgtcggt gcgggcgtcc acctcgctgt gggcgatgtc
     1681 gcaggactgg ctgcgcaacc cctggcaggc gctggactac gcgcgcctgg gcagcggcat
     1741 gacggccgag gccgccaagc tgctggtcat gcccaacgat tcgaccacgc gcttcaaggg
     1801 cacgcccggg ctggtcaagc gcgtggcctg gtcggagccg atcgacctgc gcgagatcaa
     1861 ggccgtcggc aaggtgctgg gctgctcggt caacgacctg ctgctgtcgg ccgtggccgg
     1921 cgcgctgggc gcctacctga ccgactgcgg tgacgccacc gagggcgtgg agctgcgcgc
     1981 cctggtgccg gtcaacctgc gccaggccgc agacgccggc aagctgggca accgcttcgg
     2041 catggtgacg ctggagctgc cggtcggcgt cgccaacccg ctggcgcgcc tgtacgaaac
     2101 gcgccggcgc atgcaggcca tgaagcagtc ctaccagccg gcgctgacgc tgacgctgct
     2161 gggcgccgcc ggcatgggcc ccaagctggt gcaggaacag ctgctggact ttctggccaa
     2221 caaggccacg gcggtgatga ccaacgtacc cggggcccag cagccgctgt actttgccgg
     2281 cgcgcggctg cgccagccgt tcttctgggt gccgcagtcg ggcaacatcg gcatgggtgt
     2341 gtcgatcctg tcgtacgcca accaggtgca gttcggcctc atcaccgacc gccggctggt
     2401 gcccgacccc gagcgcatcg tcagccgctt tgccgaagaa ttcgacaagc tgctgatgct
     2461 ggtgctgctg gagccgcccg agtgcctgca cgaccccgag ctggtggagg cgcacaacgc
     2521 cttctactgg tcctcggagt gaccccgttt gctacttttt tgatagcttg cggcgcttgc
     2581 tggcataggc tttgaagccg atctgaccaa aaatcatcca ggagggggtg accgacgtca
     2641 gccacccacc tcggcgcgcg ccacctccac gtccagccgc tccatggcgt cggccggcgt
     2701 gcactgcgcg gcgcgggcgt agagctgctc ggcttccttc aggcgcgact tgccgaacat
     2761 catggccagg ccgttggcgt attcggtcat ggcgatcggc gactcggggt tgagcgccag
     2821 cgcggtcttg aagtgctttt ccgccgcgtc cttcttggcg ccataggtca gcccaccgac
     2881 caccgcaccg accttgttga tgatctcggc atgccaggtg cccagcgcga tgtgggcatc
     2941 ggcgtggcgc ggctccaggt cgatggcctt ctgcaggctc tcgcgcacct tgctgcccag
     3001 tccctcggcc agcgccttgg ccaccgagat gccctggctg tagcgcccca gtgcgtagcc
     3061 gtggtagtac caggcgttga cgttgcccgg ctggctggtc tgcagcgcct cgcagcggcg
     3121 cgccacctcc tgcagcagcg ccagcttgcg cgcctggtcg tcctcgaccg aggcggcatg
     3181 gatcatggcc gccttgttgg cggcgttggt gccgctggcg ttgccggcct tcagccccag
     3241 cgccaccgcc tgcgcgaatt caccggcgtg gtaggcgcgc caggccgcct gcgcggcgct
     3301 gtcggtcggc cacggttcgg catcgccctg gtgcaggcgc gcccagtgct tcttcagcgc
     3361 ggtgccggcg taggtgtagg ccgggtcggg atggggaaag gctttccagg cggtcttgct
     3421 catcgcgcgt ctcctcgtga agtggaatgg acgtaggaca gtgacagttt ctccagcaac
     3481 cggcgcgccg gctcgcgcag ccagggcgcc gtcagcgaca gcacctggtg cgccccacgg
     3541 ccgatgtcgg gcgggcggcg cggatggcgc gcgtgctcga acgacagcca gaaggtggtg
     3601 accagcgtga tgttgaccgc cagcgcggcg atctcgtccg gcgtggcgtc cagcgcgccg
     3661 gccgctgcca ggccatggca cagggccacg gcggtctgct gcttgtgcgc caggatgcgc
     3721 tggaaatgcg tttcgaccag gcggttgcgc gtcagcaggt cgttgatgtc gcggtagatg
     3781 aagcggtagg tcgagatggt ctcgaacagc aggtgcagga agaaccacac gtcctccacc
     3841 tcgcgcggcg cggtgtcgcg cagcgtcagc agctcgctga tctcgcgctc gtagcgcgcg
     3901 aacagcgcat tgacgatctc gtccttgttg cggtagtggt agtacaggtt gccgggactg
     3961 atgttcatct cgtcggcgat caccgtggtg gtgacgttgg gctcgccgaa ctcgttgaac
     4021 aggcgcagcg cggtctccag gatccgctcg ggcgtgcgac gcttgggctt gttcatgccg
     4081 ccatttcacc acgacgcggc ggcggcccgg ccaccccgcg tgctcagctc aggccgcgcg
     4141 gcgcgcgtcg atccagtgct gcagctggtc gagcgtgcgc gccaggccct gcgtggcggt
     4201 ggccgccggc tccgccccca gcagggtgcg tgtgcggtcg cgcagcacct ccaggttgag
     4261 ctgcagccca tggcgcgcca gcaccggcgc cagctcgtcg cggcgctggt acaggtgctc
     4321 gcgcgtgcgc tggtaggcgt gttcgcacag ccgcgcgcgg tcgcggtagc tgaacaggtt
     4381 ggcgaagaac atcgcctcgt cgtcctgcgc cggctcgaac agcagcacgt cggcgtgctt
     4441 gaactggtgc tggtagcggt ccatgcccac gcgcatgcgc gagcggatga tggcgcgaaa
     4501 ggtctgtgcc agcaccaccg gcaggccgcc ttcgctgagc tgtcggtggc cggcgccgtg
     4561 ctgctcggcc agctgggcgt cgaacggcac cagcgggttg acgcacagca gcagatcgac
     4621 gccgtcgcgc agcgccaccg aggcatgcag ggtcttgatc agcgcgccgt ccacatagtg
     4681 ccggccctgg atctcgaccg gcggaaacaa ccccggcagg gcggcgctgg cctgcacggc
     4741 ggtggagatc ggcacgtcgt cgcgcccggg cccgccaaag gccaccgcct gaccggaatc
     4801 caggtcggtc gccaccacgt acaggcgcgc cttcaggcgg cggaagtcgt tgctgcggcc
     4861 aacctcggag aactgacggc gcagcaccgc atccacgccg gcaccgtcga acaggccggt
     4921 gggaatggcg cgcgacaggc gctggaacga ttcgaacagc ccatgccgcc acgggttgcc
     4981 caggtactcg cgcgccgcct gcagcgccag ccgcggaatg gcgcgggccc ggttcaggta
     5041 ctcgcgcagc gccgggcgca gcagcaggct ggggtcgaac gcctggctgc cgtcgctgcc
     5101 cagcagcatg cgcgccagcc ggcgcggctc gaagccgttg gccagcgaag ccgccacgaa
     5161 gctgccggcg ctgacgccga cgtagacgtc ggcatccacc aggctcagtc cgtcgatggc
     5221 ttcttccagc gccacgcagg cgccgacttc ataaatgccg ccgagcggac caccgccggc
     5281 cagagccaat ccgattttgg acaaagcacc tacccccaac tctgaggccg tgggttttcc
     5341 cccatgccca agccatcggt ggcgccccgt gccttcggtg gcgccaccgt ctcaggcccg
     5401 ccgcctggca ggccgaccgc tggacacgcc ctgggccatc cggcccgggc gcgcccgtgc
     5461 ggcactcaga cgctcaggcg ctcttgcgcg cggccttctt ggcgggcgca gccttcttgg
     5521 ccggcgccgg cttggccacg gccggcttgg cggccggcag gcgcggcgcg gccttgccct
     5581 tgttctgcgc cagttcctgc acggcagcgg tcagctcggc gatgcgcttg gacagcacat
     5641 cgacttcctt cttgctcggc acacccaggc tgcccagggc gcgcgccacg cggtcttcga
     5701 acacctgctc caggcggtcc caggtgtcgg cggcacggcc ggcgacggcg gcgatgcggg
     5761 cgtcggccac ttcgcgcgtc ttcttctgca tcatctcgcc ttccttgacg agggcgtcga
     5821 acatcttgcc gccctcttcc tgggccttgg caaaggcgcc caggccggcc agccagatct
     5881 gctgggtcga ttccttgatg gtggcggcga gctggttgtt gtcggagatg gtcttgagtt
     5941 tgctggccat ggtggcctcc ttccttgtgg tggtggactg cggattgacc tcttccaacc
     6001 cgctgagatg aagtctaggc caggccatta gagcaaacac actattcgtg accgcgcctg
     6061 tggcgcgctg ccgacgccgc cggcacttct agaattgccg ccctgagcat cagaaccacg
     6121 caggaggcag acatgcagta cttcatcacc ggggccaccg gtttcatcgg caagcggctg
     6181 gtggccagat tgctggagcg cgaaggcgcg cgcatcttct ttctggtgcg ccagcgcgac
     6241 cccgagcgcc tgcaggcgct gtacgacttc tggggctgcg acgacacccg ggccaccccg
     6301 gtggtgggcg atctgacgca ggacatgctg ggcgtgtcgg cggccgaccg gcgcaagctg
     6361 ggcaagaaga ccacacattt ctttcacgtg gcggccgtct acgacctgag cgccgacgcc
     6421 gaacgacagc tccaggtcaa cgtggagggc acgcgtcagg cggtgcgctt tgccgaggcc
     6481 atcggcgcca agcatttcca cctgttcagc tcgatcgccg ccgccggcct gtacgagggc
     6541 gtgttccgcg aagacatgtt cgagcaggcc gaggggctgg atcaccccta cttccgcacc
     6601 aagcacgatt cggaaggcat cgtgcgcaac gaatgccgca ttccctggcg cgtgtaccga
     6661 ccggccatcg tggtgggcga ttcgcgcacc ggcgaaatgg acaagatcga cggcccgtac
     6721 tacttcttca agctgatcca gaagctgcgc cgcctgctgc cagcctggat gccggccatc
     6781 ggcatcgagg gcgggcgcat caacctggtg ccggtggatt acgtggtgga cgcggtggac
     6841 cacatcgcgc acctgaaggg cgaggacggg cgctgcttcc acctggtcga tccgcgccca
     6901 cgccgcgtgg gcgacgtgct caacatcttc gcccgcgccg gtcatgcgcc gcagatgtcg
     6961 gtgcgcgtca acgccgccct gctgggcttc atccccaaat acatgcgcaa ggcgctgctg
     7021 gcgctgacgc cggtgcggcg cgtgcgcgac gtgctgatga aggagctggg cctgcccgac
     7081 gacatcctga gcttcgtcaa ctacccgacg cgcttcgaca gccgcgaaac cgagcgcgtc
     7141 ctcaagggca cgcgcatcgc cgtgccgccg ctggaagact acgcctggcg cctgtgggac
     7201 tactgggagc gctgcctcga ccccgacctg ttcatcgacc gcagcctggg cgggcgcgtc
     7261 aagggtcagg tggtggtggt caccggcgcc tcctcgggca tcggcaaggc ggtggcgctg
     7321 cggctggccg aagccggcgc catcgtggtc accatggcgc gcggcaaacc cgcgctggac
     7381 gccgccgcca gcgagttcga ggcgctgggc ctgaagctgc acacctatga agccgatctg
     7441 gccgacctgg cgcaggtgga cgcggtggca cagcaaatcc tgcacgacca cgggccggtg
     7501 gcgatcctga tcaacaacgc cggccgttcg atccgccgcg ccatcgaaaa ctcctacgag
     7561 cgctttcacg acttcgagcg caccatgcag ctcaactact tcggcgcgct gcggctgaac
     7621 ctgaacctgc tgccggcgat ggccgagcga cgcaccggcc acatcatcaa catctcctcc
     7681 atcggcgtgc tgaccaacgc gccgcgcttt tccgcctacg tggcgtcgaa ggcggcgctg
     7741 gacgcctgga cgcgctgcgc ggcctcggaa ttcgccgacc gcggcatcca cttcaccacc
     7801 atcaacatgc cgctggtcaa gacgccgatg atcgcgccca ccgcgctgta cgaccaggcg
     7861 cccaccctga cgccggaaga ggcggccgac atggtgtgcg aagccatcgt gcaccggccc
     7921 acccgcatgg cgacccgcct gggcatcacc ggcgaggtgc tgcacgcgct gatgccgcgc
     7981 gtggcgcagg cggtgatgaa cacctcgttt cgcatgttcg gcgagtccca ggccgccgcg
     8041 agcggcgaaa agaccgcgcc gacgcgcgat cagctggccc tgcagaactg gatgcgcggc
     8101 atccacttct gacgccgaca cacgcaccgg cccctggtat tcaatgcttt ttcgggctcc
     8161 ggcgcttgcc tagcaagcgc cggcagctat tgtttttata gtttcgtgtg cgccagcacc
     8221 agccgcaagg tgcgctcgtc gcacagcgcc gccatgtgcc ccacgccggg cagcggctcg
     8281 tcgcgcgcgt cggcgtggcg ctgcagctgc tgcggcatga cgaagttgtc ctgcggcgtg
     8341 cggatcgaca cgaagggcac gttcacgcgc tgctgcgcca gccgcgccag ccagtgcgag
     8401 ccgggctgca tctgccgggc gcagcggccc aggcccaggt gcgccaggcg gctgccgccg
     8461 tgcgggctgg cgatgctgat cagagcggcc acccgcgcag caccgtggcg cgccaggcag
     8521 gcgcgcgaca ccagcccgcc catgctgtgc gccaccaggg tcaggcgcgg cgcaccggtg
     8581 gcggcgcaca cggcctcgat gcgctgctgc aactgctcgg caaagccgtc gatgtcgccc
     8641 cagggcggct ccaggctgag cgtggccacc acgtgaccgg cggcctccag gcgcggccgc
     8701 agccaccacc agatggcgcg gttgcagccg tagccatgca ccagcaggat gggctgggcg
     8761 gacgggcgca gccgatcggc cggcatggcc aggcgcgccc agggctgaaa cagcacgaac
     8821 ccgcccacga aggcccagta ttcgcgccac agcatgacca gccaggcgcc cgccgacagc
     8881 ggcggcgccg ccgagcgatg gcgccaggcc accagccagg tgacggccag cagcagcgcc
     8941 cgccccagca gcagcgcgcc cagcgccagc gccagcgtgg cgccccacga ccagcccagc
     9001 cccgcgcgcc cgatggcgcc ccagaccagc agctcgaccg ccattcccca cagcaacagc
     9061 gccgacagca tgcgcaggat ctcccttctt gatgtaccag cctttcagtc tagtccactg
     9121 aaacattgat ccaccatgcc tgcggccacg cgccgcgcag ctggcccctt ggcgactcgc
     9181 tgtaagtacc tgacggcgcg aaacactgca actaggcgac cgcttctaaa cccgggcgcg
     9241 gcggcttcgg ataatgcctg catgagcgag accaccgaca cggtcagcga gcggcaggcc
     9301 tgcacccacg cgctgaccca ctggttgacc gaggcgcgcc atcagcgcca gcgtgccgct
     9361 gccgagccac ggcgcgccgc ccgccggcag gccctgcgcg cctttcaggc cgcacggctg
     9421 gcgcgcaccc acgccgacct gctggacagc ccgcgctacg ggcgcgccgc ggctttcttt
     9481 ctgaccgacc tgtacagccc gaaggacctg gggccgcgcg acgccgagat cgagcgcgtg
     9541 ctgccgctca tcaccagcac cctgccgacc gcgggcctgc acgcgctgct gcaggcggcc
     9601 gagctggacg cgctgtccga acgcttcgac gccgccatgg tccatgccct gggcgagcgg
     9661 ctggacacca gcctcgacga cgccgactac gccgccgcct accgcaccgt ggacgatgcg
     9721 cccggccgcc agcaccagat cgcgctgatc ctgggcaccg gccagacgct ggaccggctg
     9781 gcacacaagc ccggcctggc ggcgctgctg aagatgatgc gcggcccggc gcaactggcc
     9841 gggctgggcg agctgcagtc gtttctcgag cgcggcttca ccgcctttcg cagcatgcgc
     9901 agcgcgcagg cgtttctgga caccatcatc gagcgcgagc gcgcgctgtc ggcacggctg
     9961 ttcggcggtg gccagagcct ggcgtgagcc tgcggcgaca atgccgcatg tttgccccca
    10021 gccaagccga cgtccgccgc ttcttctgcc acgcctgggc caaggcccgc ggcgcccagc
    10081 ccatggacgc catcgagcag ctggccgccg gctggatcgc cgagcacccc gagtggcacg
    10141 ccgatttcgc cgacgccgaa gcggccgtgg cgcgccacta cggtgacggc gcggtgggcg
    10201 gcaacccgtt tctgcacctg tcgatgcacc tgtcgatcag cgagcagtgc agcatcgacc
    10261 agccgcgcgg catccgccag gcggtggagc tgctggcgca ccgccgcggc gacctgcacg
    10321 ccgcgcacca cgaggtgatg gaatgcctgg gtcagatgct ggccgatgcg cagagccgcc
    10381 gcgccgcccc ggacggcgac ggctacgtgg cctgtgtgca gcgccgcgca acccgcgacg
    10441 gcgcctgacc ccggacggcc gtcttcaggc ggcttcgcgt tcgaaccatg cgaccaacga
    10501 cacccgcagc gccggcatga cccatgaccc gcgggtgatg aaacctggaa acggcggttg
    10561 accgcttgtc atcttcgggc aggtcgcaga gcgctggcgg tcaacgagcg tgcccgggcc
    10621 ccatccaatc actcctgttt tgatagcttg tggcgcttgc tgggcgtgcg ctggaggcac
    10681 aaatgatgaa aaggcccgca cgcggcgggc ctggctggat gggcggcgct ggcgcgctca
    10741 gcgaaagcgc gactgcggca cggtgcgcac ttcacccggc aggctgccga tgtagcgcgc
    10801 catggccttg agctcggggt cggagaactg cttggccatg ccggccatca ccgggttgcc
    10861 acggccccag gtggcgtggc tgtcgtcgcg gtaggcgcgc agcgcggcgt acaggtagtc
    10921 ggggtactgg ccggcgatct tggggtagct gggatcgatc ggcttggaat agttgtcgcc
    10981 atggcaggac acgcaggcgc ccttttgcag cagctgggcc acctgcgcgc tcggttcgcg
    11041 cggcgcgggc agctcggtgg cgccttcgac cttgcccagc gcctcgtagt aggccgccac
    11101 gtcggcgatg tcctgatcgc tcatggagcc ggcgatgccg cgcatgctgg ggtgcttgcg
    11161 cgtgcccttg cggtattctt ccagcacggt gccgatgtac ttggcgctct ggccggcgat
    11221 cttgggcacg cgatagacct cggggaagct cgacttgtag cccgcgatgc cgtggcagcc
    11281 gatgcacatg gcgaccttgc cctcaccggc ggtggcgttg ccctggacct gcgcctgcac
    11341 cggcagcgtc atcgccaggg cggtgccgag tgcagcgaac atcggagaca ggctgagttt
    11401 cattttgcgc gcacaatcca tgttgtttga tccagatcag aggcggattg taatcatcac
    11461 gcttgcgctt tcgacccctg ggtttccccg ctatccacac cgctgtccga catgaaattc
    11521 caaggcaccg actcctacgt cgccacgcaa gacctgatgc tggccgtcaa cgcctccatc
    11581 acgctcaagc gcccgctgct ggtcaagggc gagcccggca ccggcaagac catgctggcc
    11641 gaagaggtgg cgcaggcgct gggcctgccg ctgctgcagt ggcacatcaa gagcaccacc
    11701 aaggcgcagc agggcctgta cgaatacgac gccgtgagcc gcctgcgcga cagccagctg
    11761 ggcgacgacc gcgtcaagga catccacaac tacatcgtca agggcgtgct gtggcaggcc
    11821 ttcaccgccg acgagccggt ggccctgctg atcgacgaga tcgacaaggc cgacatcgaa
    11881 tttcccaacg acctgctgcg cgagctcgac cgcatggagt tccactgcta cgagacgcgc
    11941 gaaaccatcc gcgccaggca tcgcccggtg gtgttcatca cctccaacaa cgaaaaggag
    12001 ctgcccgacg ccttcctgcg ccggtgcttc ttccactaca tcaagttccc cgacgccgac
    12061 accatgcggc agatcgtgga cgtgcacttc cccgacctca agaaggaact gctggccgcc
    12121 gccatgaagg tgttcttcga cgtgcgcggt ctgccgggcc tgaagaaaaa gccctccacc
    12181 agcgagctgc tcgactggct caagctgctg gtggccgagg acatcccggc cgaggcgctg
    12241 caaagccagg acgacaaggt cgccgtgccg ccgctggtgg gcgcgctgct caagaacgag
    12301 caggacgtaa ccctgttcga gaagctggtg ttcatgaacc agcgtcaccg ctgaacctcc
    12361 gggagccggc gccatgcatc cgctgctggt cgagggtctg tccgacgcgg tcggcttcgt
    12421 cggtggcgcg ctggccggct tctggctggc gcggctgctg ggtttcgacg tcttctccga
    12481 aggctacgac agcgccagcg tgctggcgat cgccgccgtc ggcctgggcg gtggcctggg
    12541 gctgggtgcg gcgcgccgct ggcgccgcgc gcgccaggcc gagcgcaagg agcgctgagc
    12601 catggccttc gtcgaacccg tcacgctgcg cggccacggc ctgcgcctgg agccgctggc
    12661 gctgacgcac gaggccggcc tggccgcggc cgccgccgac ggggcgctgt ggcgcatccg
    12721 cgtcacctcg gtgcccgagc cccaggaaac ccgcgcctac atcgaaaccg cgctgcagat
    12781 gcgcgaagcc ggatcgcgcc ttgcctttgc cgtgctcgac gacgccagcg gccgcatgct
    12841 tggcagcagc agctaccacg acatcctgcc ggccgtgcgc cgggtcgaga tcggctacac
    12901 ctggtacgcc aggagcgtgc agcgcacgca cgtcaacacc gccgccaagc tgctgctgat
    12961 gggccacgcc ttcgacaccc tgggctgcca tgtggtgggc tggcgcaccg acaacttcaa
    13021 ccacgccagc cagcgcgcca tcgaacgcct gggcgccagg aaggacggcg tgatccgcgg
    13081 ccacgcgctg cgccgcgacg gcaccatccg cgacaccgtg atgtacagca tgcgcagcgg
    13141 cgaatggccc gaagcgcgcg cccagttgct ctacttattg gagctgcggg cgcaagcccg
    13201 gtaagcgctg gaggcctttt tcatgctgat cgatttcttc tacgccctgc gcgccgccaa
    13261 gctgccggtg tcggtcaagg agttcctcac cctgctcgaa gcgctgcagg ccaacgtggt
    13321 cggcccccgg cagcccgacg cctgcacgat ggacgacttc tactacctgg cgcgcaccgc
    13381 gctggtcaag gacgagaagc atttcgacaa gttcgaccgc gccttcgccg cctacttcaa
    13441 gggcgtcgag atgctgaccg acttcagcaa ggagatcccg gccgactggc tgcgcaagat
    13501 gctggagcgc gagctcacgc ccgagcagaa ggccgccatc gagaagatgg gctgggacga
    13561 gttgatggag actttgagaa agcgcctgga agagcagaag ggccgccacg aaggtggcag
    13621 caagtggatc ggcaccggcg gcaccagccc cttcggccac ggcggctaca acccgcaggg
    13681 cgtgcgcatc ggcggcaagg gcggcaacag gagcgccgtc aaggtgtggg accagcgcgc
    13741 ctaccgcgac tacgacgacc agcag
//
LOCUS       DAOOOG010000054        13638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4931_length_13638_cov_30.335051, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000054 DAOOOG010000000
VERSION     DAOOOG010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4931_length_13638_cov_30.335051"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2369)
                     /gene="carB"
                     /locus_tag="PK242_05860"
     CDS             complement(<1..2369)
                     /gene="carB"
                     /locus_tag="PK242_05860"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOZ93576.1"
                     /translation="MPKRTDIQSILIIGAGPIVIGQACEFDYSGVQACKALREEGYRV
                     ILINSNPATIMTDPATADATYIEPITWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     WRHGVLAKYNVELIGAKPEAIDKAEDRLKFKDAMTRIGLESARSGIAHSMDEAWAVQK
                     RVGFPTVIRPSFTLGGTGGGVAYNPEEFETICKRGLEASPTNELLIEESLLGWKEFEM
                     EVIRDKADNCIIICSIENLDPMGVHTGDSITVAPAQTLTDKEYQLMRNASLAVLREIG
                     VDTGGSNVQFAVNPADGRMIIIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNDITGGATPASFEPTIDYVVTKIPRFAFEKFPAADSHLTTQMKSVGEVMAIGRTFQ
                     ESFQKALRGLEVGVDGMNEKTQDREWLEKELGEPGPDRIWFVGDAFAAGWSLDEVHQL
                     TRIDKWFLVQIEEIVKIELELDRHTEQQGDRALAALSKAELLALKQKGFSDRRLAKLL
                     KTSDKAVRDARRALGVRPVYKRVDTCAAEFATHTAYLYSTYEGECEAEPTHQKKIMVL
                     GGGPNRIGQGIEFDYCCVHAALAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTL
                     EDVLEIVDKERPLGVIVQYGGQTPLKLALGLEAEGVPIIGTTPDMIDAAEDRERFQQL
                     LHELNLRQPPNATARTEAEALAKASELGYPLVVRPSYVLGGRAMEIVHEQRDLERYMR
                     EAVKVSNDSPVLLDRFLSDAIECDVDAVRDSAGRVFIGGVMEHIEQAGV"
     gene            complement(2362..2724)
                     /locus_tag="PK242_05865"
     CDS             complement(2362..2724)
                     /locus_tag="PK242_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93577.1"
                     /translation="MKLYAFPQATLEKAIARRMLGLASPHREWFADRWSQKPYKKSFL
                     EHKAMPLVTLLAKGKTWTDEEWDRELAAWDVRFYDAEAEVLRPLIEGDGLIQLMQKNM
                     PPQRAQALLDKLNHDRHA"
     gene            complement(2721..3932)
                     /gene="carA"
                     /locus_tag="PK242_05870"
     CDS             complement(2721..3932)
                     /gene="carA"
                     /locus_tag="PK242_05870"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOZ93578.1"
                     /translation="MLSSLQGKFPPAILALADGSVFIGQSIGAPGTTVGEVVFNTAMT
                     GYQEILTDPSYCRQIVTLTYPHIGNVGVNAEDVESARVQAAGLVIKDLPLLVSNFRAE
                     RSLSEYLRAEGTVAIAGIDTRKLTRLLRTHGAQNGAIVGLAAGEAVTPERVAATVAAA
                     REAPAMTGLDLARVVSTDRAYEWNQTEWHLGAGFGTQQAPRFHVVAYDFGVKKNILRM
                     LAARGCRVTVVPAQTPAAEVLAQRPDGVFLSNGPGDPEPCDYAIAAARTLIDSGKPVF
                     GICLGHQIMALASGARTYKMKFGHHGANHPVKDLDDGRVSITSQNHGFAVDAATLPAN
                     LRPTHVSLFDGSLQGLSFTDQPAFCFQGHPEALPGPHDVGYLFDRFIQNMEQTGAGAA
                     PNAIQTGVATA"
     gene            complement(4120..4896)
                     /locus_tag="PK242_05875"
     CDS             complement(4120..4896)
                     /locus_tag="PK242_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOZ93579.1"
                     /translation="MRPAEPLRVQSRDNALFKDLRRLAQDSGAWRRQGRLWVEGDHLC
                     RAALARGWRPELAVFSESFWPLAPAQLAQAAIKNVVFADGLMGALSALESPPPMAFVL
                     PLPAAPALAPDAPTVVLDRVQDAGNVGSILRSAAAFGFTQVAALSGTAGLWSPKVLRA
                     GMGAHFGLRLVDALAVPELAALRVPLLVTSSHQGQWLHRASLPWPCAWVLGHEGQGVQ
                     PALVERAALALRIAQPGGEESLGVAAAAAICLHASAVARP"
     gene            complement(4893..5528)
                     /gene="rnhB"
                     /locus_tag="PK242_05880"
     CDS             complement(4893..5528)
                     /gene="rnhB"
                     /locus_tag="PK242_05880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOZ93580.1"
                     /translation="MRSKHFLRAEQAALDWDTPGLLAGVDEAGRGPLAGPVVAAAVIL
                     DPRQPIAGLADSKKLTARRRERLYDEIRAKALCCAVAEASVEEIDRLNILQATLLAMQ
                     RAVAGLRLPPAKVLIDGNRLPQLAVRAEPVVGGDARVPAISAASILAKVQRDRWCAEV
                     DAAWPQYGFAAHKGYGTAAHLAALRAHGACPWHRRSFAPVAAAIARAGCEA"
     gene            complement(5500..6726)
                     /gene="lpxB"
                     /locus_tag="PK242_05885"
     CDS             complement(5500..6726)
                     /gene="lpxB"
                     /locus_tag="PK242_05885"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOZ93581.1"
                     /translation="MGAPVRVAAAPRFALVAGEASGDLLAGHLLDALRARWPGLQAMG
                     VGGAEMAARGFQAWWPVDRLAVRGYLEVLPRLPALLRLRRQLGDRLLAERPDLFIGVD
                     APDFNFDLEARLKAGGIRTLHFVSPSFWAWRPEKLLKLKAAADHVLCLFPFEPALLAE
                     QGIAASYVGHPLASAIPLQSDRAAARRALGLDAQSPVVALLPGSRAAEIKHLASRFFQ
                     AAARIRQARPATQFIVPTVPERLPQVQALAAASGLGASLAVLRGQSHAALAACDVTLI
                     ASGTATLEAALFKRPMVIAYAMPALSYRLMRARRQLPWVGLPNILCAPPEWLHRPAGL
                     TAQQAADRPDAPFVVPELLQHAATPAALAQAVLDWLAAPEKIAATEARFAALHHDLRR
                     DTARLATDAIEALFAR"
     gene            complement(6731..7519)
                     /gene="lpxA"
                     /locus_tag="PK242_05890"
     CDS             complement(6731..7519)
                     /gene="lpxA"
                     /locus_tag="PK242_05890"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOZ93582.1"
                     /translation="MPQIHPTALVDPRAELDPTVEVGPYAVIGPQVRIGAGSKVGAHC
                     VVDGHTTIGRDNRFFPFASIGAAPQDKKYAGEPTRLEIGDGNTIREYVTINTGTVQDA
                     GVTRLGDDNWIMAYVHIAHDCRLGSHLILANAVQLAGHVHLGDWVFLGGLTGVHQFVR
                     VGDHAMTAFQTRLAQDVPPFVTAGGNPAEAQSINAEGLRRRGFSAERITLVKQMYRLL
                     YRKGLTLEAARQQIDALRGELAEADADIALMQDFLRTATRGIVR"
     gene            complement(7877..8320)
                     /gene="fabZ"
                     /locus_tag="PK242_05895"
     CDS             complement(7877..8320)
                     /gene="fabZ"
                     /locus_tag="PK242_05895"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOZ93583.1"
                     /translation="MMDIHRILTKLPHRYPFLLVDRVLDIDLQARTIRALKNVTVNEP
                     FFTGHFPARPVMPGVLMLEAMAQACALLSFETEGISLGDDQVFYFVGIDGARFKRPVE
                     PGDQLILHAAIDRARAGIYRFNCKATVGDEVACEAAIMCTMRDVG"
     gene            complement(8380..9366)
                     /gene="lpxD"
                     /locus_tag="PK242_05900"
     CDS             complement(8380..9366)
                     /gene="lpxD"
                     /locus_tag="PK242_05900"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOZ93584.1"
                     /translation="MTLRVGAIVQALGGELFGDPDRQITRLAPLEGAQPGELSFLVNP
                     RLTQLLAATRAGCLIVGPELAEAARARGDCIVAADPHLYFARLTQLWRRLHGGAARPG
                     IHPSAVVEEGAFVDPSASVGALSFVGRGARIGAGSVLKQRVTVGEDCVIGARCLIQSG
                     AVIGGDGFGFAPHEGAWVRIEQLGAVRIGDDVEVGANTCIDRGALDDTVIEDGVKLDN
                     LIQIGHNCHIGKHTAMAGCVGVAGSARIGAHCTVGGAGMILGHLTIADHVHISSASIV
                     TRSILKPGHYTGFFPLDDNEHWEKNAAALKQLSALRERIKRLERQQTPADDA"
     gene            complement(9428..9919)
                     /locus_tag="PK242_05905"
     CDS             complement(9428..9919)
                     /locus_tag="PK242_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOZ93585.1"
                     /translation="MVVALCGAFFAAPVVLAQESARIGFVNTDRMLREAVPAKAAQSK
                     LEQEFSRREKELDDAGATLKAASERFERDASTMSESQRATRQRQLMEQDREFQRKRRE
                     FQEDLNARKQEELQQILDRANRVVKQVAEAEKYDVILQEAVYVHPRLDITDKVIKALN
                     GGK"
     gene            complement(9943..12240)
                     /gene="bamA"
                     /locus_tag="PK242_05910"
     CDS             complement(9943..12240)
                     /gene="bamA"
                     /locus_tag="PK242_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOZ93586.1"
                     /translation="MTSFATRFRLRSAASLASLLLASHLAWAVEPFTVRDIRVEGLQR
                     VEPGTVFGSLPVRVGETYNDDKGAAAIRALFGLGLFNDVRLEVSGDVLVVIVQERPTV
                     AGVEFAGNREFSNEVLQGVMRDAGLTSGRPYDRALIDRAEQELKRQYINRSLYGAEVV
                     TTVTPTERNQVRLTFNITEGRPARIQNIDIVGNQAFSDGQLKDLFDLDTGGWLSWYTK
                     SDQYSRAKLNADLEALRSFYLARGYLDFKIDSTQVAIAPDKESIGITINVTEGERFVV
                     SGVTLDGNYLEKDDEFKSLVTIRAGQAYNADQVAETTKAFTEHFGNFGYAFARVEAVP
                     EVDRANNRVAIVIRSEPSRRAYVRRIHIQGNTRTRDEVIRREFRQYESSWYDAEKIKL
                     SRDRVDRLGYFTELSVTTAEVPGAPDQVDLNVEVKEKPTGSISIGAGYSSAEKLAFTF
                     GLSQDNAFGSGNKLDLQFNSGKYNRTVVVSATDPYFTTNGVSRTVDVYYRTQKPYQDQ
                     GGNYTLVTMGVGLKFGVPVTELDTIYLGLNAERTRIKPGTNIPAAYLDYANRFGYSSS
                     AFPFTLGWSRDSRDSALAPNSGRYQRLLGELSLFGDARYAKANYQYQQYIPLSKQYTL
                     AFNGEIGWGKGLGGRPFPVFKNYYAGGLGSVRGYDQSTLGPRDVTGAFIGGTRKFVLN
                     TEFITPFPGAGNDRTLRLFGFIDAGALYGEGEKIRGDALRYSTGVGLSWISPLGPLRF
                     AFAVPLRKRPGDRIQRFQFQIGTSF"
     gene            complement(12299..13636)
                     /gene="rseP"
                     /locus_tag="PK242_05915"
     CDS             complement(12299..13636)
                     /gene="rseP"
                     /locus_tag="PK242_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOZ93587.1"
                     /translation="MLIAVHEWGHYRMAVARGVRVLRFSIGFGKTIARFKPARQRPGQ
                     DTEFVIGAIPFGGYVKMLDEREAPVDEALRHQAFNTQPLASRALIVAAGPVANLLLAV
                     LLYALVNWIGVQEPRALLAPPVAGSLADKAGLQGGELVRQAAPAGEALAPVASFESLR
                     WVLTRAALDGQDLVLEVSRGADGRGRELTLPLSTLATRDPDAALFRAIGIVSPLSSPT
                     VGEVMPGGAAERAGLQQGDVVRRVGDVPVHDGQQLRELIRASGASGPPPASPWLIERD
                     GRALTLQVRPDAHEDKGQPVGRIGAYVGSQPEMVLVRRGAADGLWAGVVRVVEVSGLS
                     LRLLGRMLVGELSLRNLSGPIAIADYAGKSASLGLTYYLAFLAFISVSLGVLNLLPLP
                     VLDGGHLMYYLWEAVTGRPVGGVWMERLQYVGMSLLLAMMAIAMYNDIVTRLG"
BASE COUNT         2021 a         4575 c         4855 g         2187 t
ORIGIN      
        1 tgcacgccgg cctgctcgat gtgctccatc acgccgccga tgaacacgcg cccggcgctg
       61 tcgcgcacgg cgtcgacgtc gcactcgatg gcgtcggaca agaagcggtc cagcagcacg
      121 ggtgagtcgt tgctgacctt cacggcctcg cgcatgtagc gttccaggtc gcgctgttcg
      181 tgcacgattt ccatggcgcg cccgcccagc acgtagctgg ggcgcaccac cagcgggtag
      241 ccgagctcgc tggccttggc cagcgcctcg gcttcggtgc gggcggtggc gttgggcggc
      301 tggcgcaggt tcagctcgtg cagcagttgc tggaagcgct cgcggtcttc ggcggcgtcg
      361 atcatgtcgg gcgtggtgcc gatgatgggc acgccctcgg cttccaggcc cagcgccagc
      421 ttgagcggcg tctggccacc gtactgcacg atcacgccca ggggcctttc cttgtcgacg
      481 atttcgagca cgtcttccag cgtgagcggc tcgaagtaca ggcggtcgct ggtgtcgtag
      541 tcggtggaga cggtctcggg gttgcagttg accatgatgg tctcgtagcc gtcttcgcgc
      601 atggcgaggg ccgcgtgcac gcagcagtag tcgaactcga tgccctggcc gatgcggttg
      661 ggcccgccgc cgagcaccat gatcttcttc tggtgggtcg gctcggcctc gcactcgccc
      721 tcgtaggtgg agtacaggta ggcggtgtgg gtggcgaact cggcggcgca ggtatcgacg
      781 cgcttgtaca ccgggcgcac gcccagggcg cggcgcgcgt cacgcaccgc cttgtcgctg
      841 gtcttcagca gcttggccag gcggcggtcg gaaaagcctt tctgtttgag cgccagcagc
      901 tctgctttcg atagcgcggc gagcgcccgg tcgccctgct gctcggtgtg acggtcgagc
      961 tccagctcga tcttgacgat ctcctcgatc tgcaccagga accatttgtc gattctggtg
     1021 agctggtgca cttcatccag cgaccagccg gcggcgaagg cgtcgccgac gaaccagatg
     1081 cggtcgggcc ccggctcgcc gagctccttt tcgagccatt cgcggtcctg cgtcttctcg
     1141 ttcatgccgt cgacgccgac ttccagcccg cgcagcgcct tctggaacga ttcctggaag
     1201 gtgcggccga tggccatgac ctcgcccacg ctcttcatct gcgtggtcag gtggctgtcg
     1261 gcggccggga acttctcgaa cgcgaagcgc gggatcttgg tgaccacgta gtcgatggtc
     1321 ggctcgaagc tggccggcgt ggcgccgccg gtgatgtcgt tcttcagctc gtcgagcgtg
     1381 tagcccaccg ccagcttggc ggccaccttg gcgatcggga aaccggtggc cttggacgcc
     1441 agcgccgacg agcgcgacac gcgcgggttc atctcgatga tgatcatgcg cccgtcggcc
     1501 gggttgacgg cgaattgcac gttggagccg ccggtgtcga cgccgatctc gcgcagcacc
     1561 gccaggctgg cgttgcgcat cagctggtat tccttgtcgg tcagcgtctg cgccggtgcc
     1621 acggtgatcg agtcgcccgt gtgcacgccc atcgggtcga ggttctcgat cgagcagatg
     1681 atgatgcagt tgtccgcctt gtcgcggatc acctccatct cgaactcctt ccagccgagc
     1741 agggattctt cgatcagcag ctcgttggtg ggcgaggctt ccaggccgcg cttgcagatg
     1801 gtttcgaact cttccgggtt gtaggccacg ccgccaccgg tgccgcccag ggtgaagctg
     1861 gggcggatga cggtcggaaa gccgacgcgc ttttgcaccg cccacgcctc gtccatgctg
     1921 tgggctatcc ccgagcgcgc cgactccagg ccaatgcgcg tcatggcgtc cttgaacttc
     1981 aggcgatcct cggccttgtc gatggcctcc ggcttggcgc cgatcagctc gacgttgtac
     2041 ttggccagca cgccgtggcg ccacaggtcg agcgcgcagt tcagcgccgt ctgcccgccc
     2101 atggtgggca ggatggcgtc ggggcgctcc ttggcgatga tcttctcgac cgtctgccag
     2161 gtgatgggct cgatgtaggt ggcgtcggcc gtggccgggt cggtcatgat cgtcgcgggg
     2221 ttgctgttga tcaggatgac gcggtagccc tcctcgcgca gcgccttgca ggcctgcacg
     2281 ccggagtagt cgaactcgca ggcctggccg atgacgatgg ggccggcgcc gatgatgagg
     2341 atggattgga tatcggtgcg tttaggcatg gcggtcgtgg ttcagcttgt cgagcagggc
     2401 ctgggcgcgc tgcgggggca tgttcttctg catcagttgg atcaggccgt cgccctcgat
     2461 cagcgggcgc agcacttcgg cctcggcgtc gtagaagcgg acgtcccagg cggccagttc
     2521 gcggtcccac tcctcgtcgg tccaggtctt gcccttggcc agcagggtga ccagcggcat
     2581 ggccttgtgc tccaggaagg acttcttgta gggcttttgc gaccagcgat cggcaaacca
     2641 ttcgcggtgc ggcgacgcca ggcccagcat gcggcgggcg atggcctttt ccagggtggc
     2701 ctgcgggaag gcgtacagct tcatgccgtg gccactcctg tttgaatagc gtttggcgca
     2761 gcaccggcgc cggtttgctc catgttttga atgaaacgat cgaacaggta gccgacgtcg
     2821 tgcggcccgg gcagcgcctc cggatggccc tggaagcaga acgccggctg gtccgtgaag
     2881 ctcaggccct gcaggctgcc gtcgaacaga ctgacgtggg tggggcgcag gttggccggc
     2941 agggtggcgg cgtcgaccgc gaagccgtgg ttctggctgg tgatcgacac ccggccgtcg
     3001 tccaggtcct tgaccgggtg gttggcgccg tggtggccga atttcatctt gtaggtgcgc
     3061 gcgccgctgg ccagggccat gatctggtgc cccaggcaga tgccgaacac cggcttgccg
     3121 ctgtcgatca gggtgcgcgc ggcggcgatg gcgtagtcgc agggctcggg gtcgccgggg
     3181 ccgttggaca ggaacacgcc gtcgggccgc tgggccagca cctcggcggc cggggtctgc
     3241 gccggcacca cggtgacgcg gcagccgcgc gcggccagca tgcgcaggat gttcttcttg
     3301 acgccgaagt cgtaggccac cacatgaaag cgcggcgcct gctgggtacc gaagccggcg
     3361 cccaggtgcc attcggtctg gttccactcg taggcacggt cggtgctgac cacccgcgcc
     3421 aggtccagcc cggtcatggc gggcgcctca cgcgcggcgg cgacggtggc ggcgacgcgc
     3481 tcgggcgtca ccgcctcgcc cgccgccagg ccgacgatgg cgccgttttg cgccccatgc
     3541 gtgcgcagca ggcgcgtgag cttgcgggtg tcgatgccgg cgatggccac cgtgccttcg
     3601 gcgcgcaggt actcgctcag cgagcgttcg gcgcggaagt tgctgaccag cagcggcagg
     3661 tccttgatga ccaggccggc ggcctgcacg cgggcggatt cgacgtcttc cgcgttgacg
     3721 cccacgttgc cgatgtgcgg gtaggtcagg gtgacgatct ggcggcagta gctggggtcg
     3781 gtcaggattt cctggtagcc ggtcatggcg gtgttgaaca ccacctcgcc gaccgtggtg
     3841 ccgggggcac cgatggattg accgatgaag acactgccgt ccgcgagggc caggatggcg
     3901 ggcgggaatt ttccctggag agacgaaagc acggggttct ccgatggatg tcggtcgccc
     3961 gggcgcgcgg ctgggacgca gggcgcgtcg caacggcttc tctgagaggg gatggactgc
     4021 tttggtgcgc tcgggcgacg cggtttcgag caaagccact cattatattc cgcccccctg
     4081 atccgaccgc tggggcgggg ttttggccgg cagctggcgt cagggccgcg ccaccgcgct
     4141 ggcgtgcagg caaatggccg ccgccgccgc cacacccagc gattcttccc cgcccggctg
     4201 ggcaatgcgc agtgccagcg cggcgcgttc gaccagcgcc ggctgcacgc cctgcccttc
     4261 gtgccccagc acccaggcgc agggccaggg cagcgacgca cggtgcagcc actggccctg
     4321 gtgcgagctg gtcaccagca gcggcacgcg cagcgcggcc agctcgggca ccgccagcgc
     4381 atcgaccagc cgcaggccga aatgcgcccc catgccggcg cgcagcacct tgggcgacca
     4441 cagcccggcc gtgccgctga gcgccgccac ctgggtgaag ccaaacgccg cagcactgcg
     4501 caggatggag ccgacgttgc cggcgtcctg cacgcggtcg agcaccacgg tgggggcgtc
     4561 gggcgccagc gccggcgccg ctggcagagg cagcacgaag gccatcggcg gcggcgattc
     4621 cagcgcgctc agggcgccca tcaggccatc tgcaaacact acgtttttaa tagctgcttg
     4681 cgcaagctgg gcgggcgcca gaggccaaaa tgattcagaa aacacggcca gctcagggcg
     4741 ccagccgcgg gccagggcgg cgcgacacag gtggtcgcct tccacccaca gacggccctg
     4801 ccggcgccag gcgccgctgt cctgcgccag ccgccgcagg tccttgaaca gtgcgttgtc
     4861 gcgcgactgg acgcgcagcg gctcggccgg cctcatgcct cgcaccccgc gcgggcgatg
     4921 gccgccgcca ccggggcaaa cgagcgccga tgccacggac acgccccatg cgcgcgcagc
     4981 gccgccagat gcgccgccgt gccgtagccc ttgtgggcgg caaaaccgta ttgcggccag
     5041 gcggcatcga cctcggcgca ccagcggtcg cgctgcacct tggccaggat cgaggcggcc
     5101 gaaatcgccg gcacccgcgc gtcaccgccc accaccggtt cggcgcgcac ggccagctgg
     5161 ggcaggcggt tgccgtcgat cagcaccttg gccggcggca ggcgcaggcc ggccacggcg
     5221 cgctgcatgg ccagcagcgt ggcctgcagg atgttgaggc ggtcgatttc ctccaccgag
     5281 gcttcggcga cggcgcaaca cagcgccttg gcgcggattt cgtcgtacag ccgctcgcgc
     5341 cggcgcgccg tgagcttctt ggaatcggcc agaccggcga tcggctggcg cgggtccagg
     5401 atgaccgccg ccgccaccac cgggccggcc agcggcccgc ggccggcctc gtccacaccg
     5461 gccagcaggc cgggtgtgtc ccagtccagg gcggcttgtt cagcgcgcaa aaagtgcttc
     5521 gatcgcatcg gtggccaggc gcgcggtgtc gcgccgcaag tcgtggtgca aggcggcaaa
     5581 acgcgcctcg gtggcggcga ttttctcagg cgcggccagc cagtccagca ccgcctgcgc
     5641 cagcgcggcc ggcgtggcgg cgtgctgcag cagctcgggc accacgaacg gcgcgtccgg
     5701 acggtcggcc gcctgctgcg ccgtcaggcc ggcgggccgg tgcagccatt cgggcggcgc
     5761 gcacaggatg ttgggcaggc ccacccaggg cagttggcgc ctggcgcgca tcagccggta
     5821 gctgagcgcc ggcatggcgt aggcgatcac catcgggcgc ttgaacagcg ccgcctccag
     5881 cgtggcggtg ccgctggcga tcagcgtcac gtcgcaggcc gccagcgccg cgtgcgactg
     5941 gccgcgcagc accgcgagcg acgcacccag accgctggcg gcggccagcg cctgcacctg
     6001 gggcagccgc tcgggcaccg tcggcactat gaattgtgta gctggccgcg cttgccggat
     6061 gcgcgccgcg gcctgaaaaa accttgaagc cagatgcttg atctcggccg cgcggctgcc
     6121 cggcagcagc gccaccacgg gcgattgcgc atccagcccc agggcgcggc gcgccgcggc
     6181 gcggtcgctc tgcagcggga tggcgctggc cagcgggtgg ccgacgtagc tggcggcaat
     6241 gccctgctcg gccagcaacg ccggctcgaa cgggaacaga cagaggacgt ggtcggcggc
     6301 ggctttcagc ttgagcagct tctcgggccg ccaggcccag aacgaggggc tgacgaagtg
     6361 cagggtgcgg atgccgcccg ccttcagccg tgcttcgagg tcgaagttga agtcgggcgc
     6421 atcgacgccg atgaacagat cgggccgctc ggccagcagc cgatcgccca gctggcggcg
     6481 caggcgcagc agcgcgggca ggcgcggcag cacttccagg tagccgcgca ccgccagccg
     6541 gtcgaccggc caccaggcct gaaagccgcg cgccgccatc tcggcgccgc ccacgcccat
     6601 cgcctgcagg ccgggccagc gcgcacgcag cgcgtccagc agatggccgg ccagcaggtc
     6661 gcccgaggct tcgccggcga ccagggcaaa tcggggggcg gcggcgacgc gaaccggcgc
     6721 acccatggcg tcagcgcacg atgccgcgcg tggccgtgcg caggaagtcc tgcatcagcg
     6781 cgatgtcggc atcggcctcg gccagctcgc cgcgcagggc gtcgatctgc tggcgcgccg
     6841 cttccagcgt caggcccttg cggtagagca ggcggtacat ctgcttgacg agcgtgatgc
     6901 gctcggccga aaagccgcgc cggcgcaggc cctcggcgtt gatgctctgc gcctcggccg
     6961 ggttgccgcc ggcggtgacg aagggcggca cgtcctgcgc cagccgggtc tggaaggccg
     7021 tcatggcgtg gtctccgacg cgcacgaact ggtgcacgcc ggtcagccca cccaggaaca
     7081 cccagtcgcc cagatgcaca tggccggcca gctgcaccgc gttggccagg atgaggtggc
     7141 tgcccagccg gcaatcgtgc gcgatgtgca cgtaggccat gatccagttg tcgtcgccca
     7201 ggcgcgtgac gccggcgtcc tgcacggtgc cggtgttgat ggtgacgtac tcgcggatgg
     7261 tgttgccgtc gccgatctcc agccgcgtgg gctcgccggc gtacttcttg tcctgcggcg
     7321 cggcgccgat gctggcgaac gggaagaagc ggttgtcccg cccgatggtg gtgtggccgt
     7381 cgaccacgca gtgcgcgccg accttgctgc cggcgccgat gcgcacctgc gggccgatca
     7441 cggcgtaggg accgacctcg accgtggggt cgagctcggc gcgcggatcg accagcgcag
     7501 ttggatggat ctgtggcatt tcaacctaga aaccgcagtt ggatgcgcct gccagtgccg
     7561 tgatcggtat gccaggcaag gcgcgacgac gcggcgggct ggtgcccgca agaaggagca
     7621 acgccgcatg gcgcgccgag tgcggtgctg gcgggtgcat agcgccttca cctggaaggt
     7681 gaaggctccg aagcggcgca agctcagagt tcatgcggca ttcccaaggg ttgcgagcgg
     7741 tcaactgcgg cttctaggtt caatggtccg acggccgcgg agcaggccat gccagcgaac
     7801 gccgtggccg gacctgcgcc ggccactggc gttgtccccc cccgcggggg gaaggcgcgc
     7861 agcgactcag gggggttcaa cccacgtccc gcatggtgca catgatggcg gcctcgcagg
     7921 ccacctcatc gccgaccgtg gccttgcagt tgaagcggta gatgccagcg cgcgcgcggt
     7981 cgatggccgc gtgcaggatc agctggtcgc cgggctcgac cgggcgcttg aagcgcgcgc
     8041 cgtcgatgcc gacgaaatag aacacctggt cgtcgcccag cgatatgccc tcggtctcga
     8101 acgacagcag cgcgcaggcc tgggccatgg cctccagcat cagcacgccc ggcatcaccg
     8161 ggcgggccgg aaagtggccg gtgaaaaagg gttcgttgac ggtgacgttc ttgagcgcgc
     8221 ggatggtgcg cgcctgcagg tcgatgtcga gcacgcggtc aaccagcagg aaggggtagc
     8281 ggtgcggcag cttggtcagg atgcgatgaa tgtccatcat ggcgttgctt ctatttcgat
     8341 agctggttgc gcccgatcaa cgggcgcgcg tggcccattt tatgcgtcat cggcgggtgt
     8401 ctgctggcgt tccagccgct tgatgcgctc gcgcagcgcg ctgagctgct tgagcgcggc
     8461 ggcgttcttt tcccagtgtt cgttgtcgtc cagcggaaag aagccggtgt agtgcccggg
     8521 cttgaggatg gagcgcgtga cgatcgaggc cgatgagatg tgcacgtggt cggcaatggt
     8581 caggtggccc aggatcatgc cggcgccgcc caccgtgcag tgcgcaccga tgcgcgcgct
     8641 gccggccacg cccacgcagc cggccatggc ggtgtgcttg ccgatgtggc agttgtggcc
     8701 gatctggatc aggttgtcga gcttgacgcc gtcttcgatc acggtgtcgt cgagcgcgcc
     8761 gcggtcgatg caggtgttgg cgccgacttc cacgtcgtcg ccgatgcgca ccgcgccgag
     8821 ctgttcgatg cgcacccagg ccccttcgtg cggggcgaag ccgaagccgt cgccaccgat
     8881 caccgccccg ctctggatca ggcagcgggc gccgatcacg cagtcctcac ccacggtgac
     8941 gcgctgcttg agcacgctgc cggcgccgat gcgggcgccg cggccgacga agctgagtgc
     9001 ccccacgctg gcgctggggt cgacgaaggc gccctcttcc accaccgcgc tcgggtgaat
     9061 gccgggccgc gccgcgccgc cgtgcaggcg ccgccacagt tgcgtcaggc gggcgaagta
     9121 cagatgcgga tcggccgcca cgatgcagtc gccccgggcg cgcgccgcct cggccagttc
     9181 ggggccgacg atcaggcagc cggcgcgggt ggcggccagc aactgggtca ggcgcgggtt
     9241 gaccagaaaa ctcagttcgc cgggctgcgc gccttccagc ggcgccaggc gcgtgatctg
     9301 gcgatccggg tcgccgaaca gttcgccgcc cagggcctgg acgatggctc cgacacgcag
     9361 ggtcatggcc gcacgctcat ggacaggaca gccaccacgg tccggggcgc ggcacgccag
     9421 gccccggtca cttgccgccg ttcagcgcct tgatcacctt gtcggtgatg tcgagccgcg
     9481 gatggacata caccgcctcc tgcaggatga cgtcgtactt ctcggcctcg gccacctgct
     9541 tgacgacacg gttggcgcgg tcgaggatct gctgcagctc ttcctgcttg cgtgcgttca
     9601 ggtcttcctg gaactcgcgg cgcttgcgct ggaactcgcg gtcctgctcc atcagctggc
     9661 gctggcgcgt ggcgcgctgg ctctcgctca tcgtcgaggc gtcgcgctcg aagcgctcgg
     9721 acgccgcctt gagcgtggca ccggcgtcgt cgagctcctt ctcgcggcgc gagaactcct
     9781 gctccagctt ggactgcgcg gccttggcgg gcaccgcctc gcgcagcatg cggtcggtgt
     9841 tgacaaagcc gatgcgcgcg ctctcctgag ccagcacgac cggcgcggca aagaaggcgc
     9901 cacacagcgc tacgaccatc agacggcgag acatggactg cttcaaaacg aggttccgat
     9961 ctggaattgg aagcgttgaa ttctatcgcc cggcctcttg cgcagaggca cggcgaaggc
    10021 aaagcgcagc gggccgaggg gagaaatcca gctcaggccg acgccggtgg aatagcgcag
    10081 cgcgtcgcca cggatcttct cgccttcgcc gtacagcgcg ccggcgtcga tgaagccgaa
    10141 caggcgcagc gtgcggtcgt tgccggcgcc cgggaagggg gtgatgaact cggtgttcag
    10201 cacgaacttg cgcgtaccgc cgatgaaggc tccggtgacg tcgcgcgggc ccagggtgga
    10261 ctggtcgtag ccacgcaccg agcccaggcc gccggcgtag taattcttga acaccgggaa
    10321 cggccgcccc cccagaccct tgccccagcc gatttcgccg ttgaaggcca gcgtgtactg
    10381 cttgctgagc ggaatgtatt gctggtactg gtagttggcc ttggcgtagc gcgcatcgcc
    10441 gaacaggctg agttcgccca gcagacgctg gtagcggccc gagttgggcg ccagcgcgct
    10501 gtcgcgcgaa tcgcgcgacc atcccagcgt gaacggaaag gccgagctcg aatagccgaa
    10561 gcggttggcg tagtcgaggt aggcggcagg aatgttggtg ccgggcttga tgcgcgtgcg
    10621 ttcggcgttc agacccagat agatggtgtc gagctcggtc accggcacgc cgaacttcag
    10681 gcccacgccc atggtcacca gggtgtagtt gccgccctgg tcctggtacg gcttctgggt
    10741 gcggtagtag acgtcgaccg tgcgcgagac gccgttggtg gtgaagtagg gatcggtggc
    10801 gctgaccacc acggtgcggt tgtacttgcc gctgttgaat tgcaggtcca gcttgttgcc
    10861 ggagccgaag gcgttgtcct gtgacagccc gaaggtgaag gccagctttt cagccgacga
    10921 gtaaccggcg ccgatgctga tgctgccggt aggcttttcc ttgacctcga cgttgaggtc
    10981 gacctggtcg ggcgcgcccg gcacctcggc ggtggtgacc gacagttcgg tgaagtagcc
    11041 cagtcggtcc acgcgatcgc gcgacagctt gatcttctcg gcgtcgtacc aactggattc
    11101 gtactggcgg aattcgcgcc ggatcacctc gtcgcgcgtg cgcgtgttgc cctggatgtg
    11161 gatgcgccgc acgtaggcgc ggcgcgaggg ctcggagcgg atcacgatgg ccacgcggtt
    11221 gttggcgcga tcgacttcgg gcaccgcctc cacgcgggca aaggcgtagc cgaagttgcc
    11281 gaaatgctcg gtgaaggcct tggtggtttc ggccacctgg tcggcgttgt aggcctggcc
    11341 cgctcggatg gtgaccagcg acttgaactc gtcgtccttt tccaggtagt tgccgtccag
    11401 cgtcacgccc gacaccacga agcgctcgcc ctcggtgacg ttgatggtga tgccgatgga
    11461 ttccttgtcc ggcgcaatgg cgacctgggt ggagtcgatc ttgaagtcga ggtagccgcg
    11521 cgccaggtag aacgagcgca gcgcctccag gtcggcattg agcttggcgc gcgagtactg
    11581 gtcgctcttg gtgtaccacg acagccagcc gccggtgtcg aggtcgaaca ggtccttgag
    11641 ttgcccgtcc gaaaacgcct ggttgccgac gatgtcgatg ttctggatgc gcgccggccg
    11701 gccttcggtg atgttgaagg tcaggcgcac ctggttgcgc tcggtcggcg tgacggtggt
    11761 caccacctcg gcgccgtaga ggctgcggtt gatgtactgg cgcttgagct cctgctcggc
    11821 gcggtcgatc agggcgcggt catacggccg cccggaagtc aggccggcgt cgcgcatcac
    11881 gccctgcagc acctcgttgc tgaactcgcg gttaccggcg aattccaccc cggccaccgt
    11941 gggacgttcc tgcacgatca ccaccagcac gtcgccgctg acttccagcc gcacgtcgtt
    12001 gaacaggccc aggccgaaca gcgcgcggat cgccgctgcg cccttgtcgt cgttgtaggt
    12061 ttctccgacg cgcaccggca acgagccgaa cacggtgccg ggctcgacgc gctgcagccc
    12121 ctcgacgcgg atgtcgcgca cggtgaacgg ctcgacggcc caggccagat gcgaggccag
    12181 cagcagactg gccagcgagg ccgcggagcg cagccggaaa cgtgttgcga aggatgtcat
    12241 gaaaggctac gggaaacgat ggccgcgcga gaccggggaa gagctgtcgg tgaaggggtc
    12301 aacccaggcg ggtgacgatg tcgttgtaca tcgcgatggc catcatggcc agcagcagcg
    12361 acatgccgac gtactgcagg cgctccatcc agacgccacc cacgggccgg cccgtcacgg
    12421 cctcccaaag ataatacatc aggtgccccc catcgaggac cggcagcggc agcaggttga
    12481 gcacgcccag gctgacgctg atgaaggcca gaaaggccag gtagtaggtc agccccaggc
    12541 tggccgactt gcccgcatag tcggcgatgg cgatcggtcc gctcaggttg cgcagcgaca
    12601 gctcgcccac cagcatgcgc cccagcagcc gcagcgacag gccggacact tccaccaccc
    12661 gcaccacccc ggcccacagg ccgtctgcgg caccgcggcg caccagcacc atctcgggct
    12721 ggctgccgac gtaggcgccg atgcgaccca ccggctgacc cttgtcctca tgcgcgtccg
    12781 ggcgcacctg cagcgtcagc gcgcggccgt cgcgctcgat cagccagggc gacgccggcg
    12841 gcggcccgct ggcgcccgag gcgcggatca gctcgcgcag ctgctgcccg tcgtgcaccg
    12901 gcacgtcgcc cacccgacgc accacgtcac cctgctgcag gccggcacgc tcggcggcgc
    12961 cgccgggcat cacttcgccc accgtcgggc tgctgagcgg cgagacgatg ccgatggcgc
    13021 gaaacagtgc cgcgtccgga tcgcgcgtgg ccagcgtgga cagcggcagg gtcagttcgc
    13081 gcccgcggcc atcggcgccg cggctgactt ccagcaccag gtcctgaccg tccagcgcgg
    13141 cgcgtgtgag cacccagcgc agcgactcga acgaggccac cggcgccagc gcctcgccgg
    13201 ccggcgccgc ctggcgcacc agctcgccgc cctgcaggcc ggccttgtcg gccagcgatc
    13261 cggccaccgg cggcgccagc agggcgcgcg gctcctgcac cccgatccag ttgaccagcg
    13321 catacagcag caccgccagc agcaggttgg ccaccgggcc ggcggcgacg atcagggcgc
    13381 gcgaggccag cggctgggta ttgaaggcct ggtgtcgcaa ggcctcgtcc accggcgctt
    13441 cgcgctcgtc cagcatcttg acgtagccgc cgaacggaat ggcgccgatg acgaactcgg
    13501 tgtcctgacc ggggcgctgg cgcgccggtt tgaaacgcgc gatggtcttg ccgaagccga
    13561 tggaaaagcg cagcacccgc acaccgcgcg ccacggccat gcggtagtgg ccccactcgt
    13621 gcacggcgat cagcaggc
//
LOCUS       DAOOOG010000055        13638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_4932_length_13638_cov_19.099094, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000055 DAOOOG010000000
VERSION     DAOOOG010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4932_length_13638_cov_19.099094"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PK242_05920"
     CDS             complement(<1..995)
                     /locus_tag="PK242_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ93588.1"
                     /translation="MNMARNFRSNRRLALAGTALAAICLIANNAWAQGAWPTKPVKIV
                     VPFAPGGTTDILARAIAPELSKAFGQQFIVDNRAGAGGNVGAEVVAKSPGDGYTLLMG
                     TVGTHGINRALYPKLPYDPIQDFVPITLVASVPNVMEMNADKARALGIHNVQDFLRHA
                     KAHPGKLNMASSGNGTSIHLAGELFKHMTGVFMTHIPYRGSGPALLDLVAGNMDVMFD
                     NLPSSMAQIKAGKLKPLAVTSAKRSAALPDVPTVEEAGGPALKGYEASSWFGLLAPAG
                     TPADIVNRLQQESAKALASPAVKEKLLAQGAVPGGIAPGEFALMIADEHAKWAKVV"
     gene            complement(992..1369)
                     /locus_tag="PK242_05925"
     CDS             complement(992..1369)
                     /locus_tag="PK242_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93589.1"
                     /translation="MKRALRLVDSITELKPAADAGCLAVSGSHGGISSAHYALAARPL
                     IAVFNDAGVGKDDAGLAALPFLARHGIAACTVAHTSARIGEARSTLEDGVLSHCNAQA
                     LALGIAPGQPCGQVIRHLERTGG"
     gene            complement(1417..2406)
                     /locus_tag="PK242_05930"
     CDS             complement(1417..2406)
                     /locus_tag="PK242_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOZ93590.1"
                     /translation="MSPLFKLSIAAVAALVALGAQAQAFPAPGKTVTLIVPFAAGGPT
                     DKVARDLAEALRKPLGGATVVIDNAAGAGSTIGAAKAFRAAPDGYTLLVTHIGMATTP
                     ALYRKLPYNVEEFEYLGLINEVPMVVVSKPGLPANNWTELKTWIGENKGKINLANAGL
                     GAASHLCGLMLQQALATEMTTIAYKGTAPAMTDLMGGQVDIMCDQTTNTTSQIEGKKI
                     KAFAVTTPKRITMPPVYKDLPTLQEVGMKGFDVSIWHGLYAPKGTPAAVVKALNEALQ
                     VALKDADFIKKEEALGAVVAHDDRVTPAGHKKFVTAEVAKWGAVIKAAGQYAD"
     gene            complement(2517..3767)
                     /locus_tag="PK242_05935"
     CDS             complement(2517..3767)
                     /locus_tag="PK242_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ93591.1"
                     /translation="MTEFFDALETRDPAAREAALMAALPAHVAHAQQRSAAFADILAG
                     VDAAAIGSRAALARLPVTRKGELLARQQASRAAGRDAFGGFAAVGWGAAMRRVYASPG
                     PIYEPEGTAVDYWRTARALYAAGFRAGDLVHNAFSYHMTPGAFILEAGALAVGCTVFP
                     AGTGQTEQQLQAIGELKPTAYVGTPSFLRILCEKAQESGADMRSLTKGMVSAEPFPPS
                     LRDWFAERGMTAYQSYATADAGLIAYETAARQGLVLDEGVIVEIVRPGTGDPVPEGEV
                     GEVVVTVLNPDYPLIRFGTGDLSAVLAGACPTGRTNTRIKGWMGRADQTTKIRGMFVH
                     PGQVAEVVRRFPEVARARLVVTGAMANDQMTLRVESVNVPGLAERLADAVRDVTKLRS
                     QIELVAPGSLPNDGKVIEDARSYE"
     gene            complement(3906..4739)
                     /locus_tag="PK242_05940"
     CDS             complement(3906..4739)
                     /locus_tag="PK242_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ93592.1"
                     /translation="MSTATPVAADAPAPLLVVNGIEVIYNHVILVLKGVSLTVPRSGI
                     VAILGGNGAGKTTTLRAVSNLLKGERGEVTKGTIEYKGERIENLSPADLVKRGVVQVM
                     EGRHCFAHLTIEENLLTGAYTRSDKGEIAANLEKVYNYFPRLKTRRTSQAAYTSGGEQ
                     QMCAIGRALMTNPDMVLLDEPSMGLAPQIVEEVFNIVRDLNTKERTTFLLAEQNTNMA
                     LKYADYGYIMESGRIVMDGKASDLASNEDVKEFYLGVGGGERKSFKDVKSYKRRKRWL
                     A"
     gene            complement(4831..6129)
                     /locus_tag="PK242_05945"
     CDS             complement(4831..6129)
                     /locus_tag="PK242_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ93593.1"
                     /translation="MKLGAIALAAAAIAGLAAPAVQAQEQFFPLLVYRTGQFAPLGIP
                     WADGKQDYLKLVNARGGINGVKIAFEECETAYDTAKGVECYERLKGRPGGTASFDTQS
                     TGITFAVSDKAPGDKATIVTTGYGLSQSADGRVFEWNFPLMGTYWTAADAMVQDILKK
                     EKGNLKGKKIALVYHDSPYGKEPIPLLQKRAAKDGFELLLLPVTPPGVEQKSTWLQVR
                     QQRPDYVLLWSAGVMTPAALREAQATNYARDKIYGIWWAGSDHDVKDIGAGAKGYNVM
                     TIHNTAERDKVHDDVKAQLYDKGQGTAKDASELGAMAHTRGMMISMLQVEAVRAAQEK
                     FGKGKVMTSEQVRWGLENLNLTQERLNELGFGKIMRAVKTSCDNHMGTDWTRVAQWDG
                     GGWKVVSDWYQADKSMIDPLVKEYGEKYAKEKNLKVRSCN"
     gene            complement(6227..7291)
                     /locus_tag="PK242_05950"
     CDS             complement(6227..7291)
                     /locus_tag="PK242_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ93594.1"
                     /translation="MFYRENGQFKTSYRADQQIFPILQDRVAVLLLLALAFVAVPLLA
                     SDYVFQAVLIPFLIMALGALGLNILVGYCGQISLGTGAFMAVGAYAAFNFQVRFDGMP
                     LLVSLLGGGLVAMVFGMVFGLPSLRIRGLYLAVATLAAQFFMDWLTSRAKWVTNNSSS
                     GSVSAGPLSIAGWKIDTPMEKYLLCLAILCVLALAAKNLVRSAIGREWMAIRDMDVAA
                     AVIGIRPMYAKLSAFGVSSFIVGVAGALWGFVHLGAWEPAAFSIDRSFQLLFMIIIGG
                     LGSIVGSLFGAAFFVLLPLFLNYVPHWLGLPLSTATATYLEHMIFGALIVFFLIVEPH
                     GLARLWSTARQKLRVWPFPH"
     gene            complement(7306..8235)
                     /locus_tag="PK242_05955"
     CDS             complement(7306..8235)
                     /locus_tag="PK242_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ93595.1"
                     /translation="MGFFLETLFGGLMTGMLYALVALGFVLIFKASGVFNFAQGAMVL
                     FAALAMARFSEWIPGWLGMAPGVLSVLLAIVVSILIMVGVAWLIERLALRHLVNQEPI
                     TLLMATLGIAYLLDGFGPMIFGSAVYSINVGMPKDPVFILEGMFEGGVLVSLEDFYAA
                     GIAAALVAALTVFFQFTKTGRALRAVADDHQAAQSIGIPLARMWVIVWSVAGVVALVV
                     GIIWGSKLGVQYSLSLVALKALPVVILGGLTSLPGAIIGGLIIGVGEKLSEVYLGPMI
                     GGGIENWFAYGLALALLLVRPQGLFGEKIIDRV"
     gene            complement(8254..9114)
                     /locus_tag="PK242_05960"
     CDS             complement(8254..9114)
                     /locus_tag="PK242_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ93596.1"
                     /translation="MNTIATEKIAAGARQTGAEGQFDSSSGAKKIGDVILDVQNISLR
                     FGGVKALTDISFNVREHEIRAIIGPNGAGKSSMLNCINGVYTPQEGSITFRGQTFSHM
                     NSRQVAEMGVARTFQNLALFKGMSVIDNIMTGRNLKIRSNLLLQALRWGPAEREEIAH
                     REFVEHIIDFLEIQAHRKTPVGQLPYGLQKRVDLGRALAMEPQVLLLDEPMAGMNVEE
                     KQDMSRFILDVNDEFGTTIVLIEHDMGVVMDISDRVVVLDYGKKIGDGTPDEVRGNED
                     VIRAYLGTSH"
     gene            complement(9111..11060)
                     /locus_tag="PK242_05965"
     CDS             complement(9111..11060)
                     /locus_tag="PK242_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ93597.1"
                     /translation="MAITFPRLLQKHAAERPGEPALREKEYGIWQTWSWQRALDEVRW
                     MACGLTALGFTPGQNLAIVGNNRPHVYLMFLVAQSLRGVPVPMYQDAVAAEMAFVLRD
                     AEVHFALAEDQEQVDKLLELRDAEGEEAVPGLTHIIYDDPRGLRNYRQPGLISVDELM
                     ALGRQHEQAHPQLFAELVAQGQPDDVAVILYTSGTTGRPKGVCQTHAAFVASAQGGVQ
                     TDGLNASDDVLSYLPPAWVGDFLFSMAQWLVAGFTINCPESSDTVSIDMREIGPTYYF
                     APPRVFEGMLTSVSIRMEDAARFKQRMHAYFMGVARRVGSDILDGKPVGLGDRLLYGV
                     GKLLVYGPLKNAMGLSRIRVAYTAGAAIGPDLFRFFRSIGVNLKQLYGQTETCAYVCI
                     QKNGQVKLESVGQAAPGIELKIADSGEVLVKGVSVLKEYYKRPDATAEVIDENGYFKT
                     GDAGVLDRDGHLRIIDRAKDVGKLAGGAMFAPNYIENKLKFFPQIKEAVCFGHEREQV
                     CAFINIDFEAVGNWAERQGLPYAGYVDLAGKPEVLKMMAECVEKVNADLAGEEGMEAT
                     QIARFLVLHKELDPDDDELTRTRKVRRGFIAEKYGVLIDALYAGRAEQFIETAVKFED
                     GRTGKVAATLKILDARTYPPVRSAA"
     gene            11198..11905
                     /locus_tag="PK242_05970"
     CDS             11198..11905
                     /locus_tag="PK242_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ93598.1"
                     /translation="MPLRLALHDRRRPLTEAELAGVPWLSHLKPDEYDRAAPELRVTR
                     AEPGELIFRMGRPVTYWFGVVDGLLKMSTETADGQTITYSGLTPGGWFGEGTVLKREP
                     YRYNVSALRRSLVAGLPADTFHWLLDHSIGFNRFIMSQLNERLGQFIGALEADRTPNP
                     EQRVARSIAALVNPVLFPGVGEVLRITQQELAYLVGLSRQRVNEALHALAAQGLLRIE
                     YGGLRILDLAALRSRQF"
     gene            11976..12800
                     /locus_tag="PK242_05975"
     CDS             11976..12800
                     /locus_tag="PK242_05975"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOZ93599.1"
                     /translation="MTTDTPRFHRAPRPAAAAPAPTTGGAIRLNKRLAELGLCSRREA
                     DDWIERGWVRVNGQLAVMGLKVGPQDQVTVDRRADAAQAQRVTILLHKPVGYVSGQAE
                     DGHQPAVTLIQARSHWTGDASRTRFAPAQLKGLAPAGRLDIDSTGLLVLTQDGRVARQ
                     LIGEHSDVDKEYLVRVSYGALAENVQAAFPAAQLARLRHGLALDGQPLRPALVDWQNP
                     EQLRFVLREGKKRQIRRMCELVGLTVTGLKRVRIGRVRLGQLPAGQWRYLGEHEAF"
     gene            12909..>13638
                     /locus_tag="PK242_05980"
     CDS             12909..>13638
                     /locus_tag="PK242_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ93600.1"
                     /translation="MSTQKHAFQAEVAQLLHLVTHSLYSNKEIFLRELISNASDACDK
                     LRFEALADGSLYGDQPELRVRIAFDKDARTLSIADSGIGMSEAEAIEHLGTIAKSGTR
                     EFMARLSGDQKADAQLIGQFGVGFYSGFIVADRITVETRRAGLPPEQGVRWSSGGSGD
                     FTVETITLPERGTRVILHLREDALEFADGWRIKELIGKYSDHIALPIQMEGETWEEGK
                     EEGQPGRMVKTGEWETVNQATALWT"
BASE COUNT         2134 a         4685 c         4551 g         2268 t
ORIGIN      
        1 accaccttgg cccacttggc gtgttcgtcg gcaatcatga gggcgaactc gccgggcgca
       61 atgccccccg gcacggcgcc ctgagccagc agtttctcct tgaccgccgg cgaggccagc
      121 gccttggccg attcctgctg caagcggttg acgatgtccg ctggcgtgcc ggcgggagcc
      181 agcaggccga accagctgct ggcttcataa cccttgaggg ccggaccgcc ggcctcctcc
      241 accgtcggca cgtcaggcag ggccgccgag cgcttggcac tggtcacggc cagcggcttg
      301 agcttgccgg ccttgatttg cgccatcgat gacggcaggt tgtcgaacat cacgtccatg
      361 ttgcccgcca ccaggtccag cagcgccggg ccggagccgc ggtaggggat gtgggtcatg
      421 aagaccccgg tcatgtgctt gaacagctcg ccagccagat ggatggaggt gccgttgccg
      481 ctggaggcca tgttcagctt gccggggtgg gccttggcgt gcctgaggaa gtcctgcacg
      541 ttgtggatgc ccagcgcgcg ggccttgtcg gcgttcattt ccatcacgtt gggcacgctg
      601 gccaccaggg tgatgggcac gaagtcctgg atcgggtcgt agggcagctt ggggtagagc
      661 gcgcggttga tgccatgcgt gcccaccgtg cccatcagca gcgtgtagcc gtcgcccggt
      721 gacttggcca ccacctcggc acccacgttg ccgccggcgc cggcgcggtt gtcgacgatg
      781 aactgctggc cgaaggcctt gctcagctcc ggcgcgatgg cccgcgccag gatgtcggtg
      841 gtgccaccgg gggcaaacgg caccacgatc ttgaccggct tggtcggcca ggcgccttgg
      901 gcccaggcat tgtttgctat caaacaaata gctgccagcg ctgttcccgc aagcgccaga
      961 cgccgattgg atctgaaatt ccgagccatg ttcatcctcc agtgcgttct aggtgccgga
     1021 tgacctgccc gcacggctgc cccggcgcga tgcccagggc cagcgcctgg gcgttgcagt
     1081 gactgagcac gccatcctcc agcgtgctgc gcgcctcgcc gatgcgcgcg ctggtgtggg
     1141 cgaccgtgca ggcggcgatg ccgtgccggg ccaggaacgg cagggccgcc agccccgcat
     1201 cgtccttgcc cacgccggcg tcgttgaaga cggcgatcag cggtcgcgct gccagcgcat
     1261 agtgggccga gctgatgccg ccgtgcgagc cgctgaccgc caggcagccg gcgtcggccg
     1321 cgggcttcag ctcggtgatg gagtcgacca ggcgcagcgc gcgtttcatg gcgcccgccg
     1381 cctgccctgt cacccgcctt ggtgggccag cccgcttcag tcggcgtact ggccggccgc
     1441 cttgatgacc gcgccccact tggcgacttc ggcggtcacg aacttcttgt ggccggcggg
     1501 agtgacgcgg tcgtcgtgcg ccaccacggc acccagcgct tcttccttct tgatgaagtc
     1561 ggcgtccttg agcgccacct gcagtgcctc gttgagcgcc ttgaccaccg ccgccggcgt
     1621 gcccttgggc gcgtacagac cgtgccagat ggagacgtcg aagcccttca tgcccacctc
     1681 ctgcagcgtc ggcaggtcct tgtagaccgg cggcatggtg atgcgcttgg gcgtggtgac
     1741 cgcgaaggcc ttgatcttct tgccctcgat ctggctggtg gtgttggtgg tctggtcgca
     1801 catgatgtcg acctggccgc ccatcaggtc ggtcatggcc ggcgccgtgc ccttgtaggc
     1861 gatggtggtc atctcggtgg ccagcgcctg ctgcagcatc aggccgcaca ggtgcgaggc
     1921 ggcgcccagg ccggcgttgg ccaggttgat cttgcccttg ttctcgccga tccaggtttt
     1981 cagctcggtc cagttgttgg ccggcagacc gggcttgctg accaccacca tcggcacttc
     2041 gttgatcagg ccgaggtact cgaactcttc cacgttgtag ggcagcttgc ggtacagcgc
     2101 cggcgtggtg gccatgccga tgtgcgtgac cagcagcgtg tagccgtccg gcgcggcacg
     2161 gaaagccttg gcggcgccga tggtgctgcc ggcgccggcg gcgttgtcaa tgacgacggt
     2221 ggcaccaccc agcggcttgc gcagcgcctc ggccaggtcg cgcgccacct tgtcggtggg
     2281 accgccggcg gcaaagggca cgatgagggt gacggtcttg cccggcgccg ggaaggcctg
     2341 ggcctgggcg cccagggcca ccaaggcggc gacggcggcg atgctcagct tgaacagggg
     2401 tgacatgaca gcggctcctt gaaatgagga atgaaaacag ccaatggtat cggtttgaaa
     2461 cgccccggcc gactgcgcgc gcaccgggaa atccccgagg tggcgttcgt cgagcttcat
     2521 tcgtaactgc gcgcgtcctc gatcaccttg ccgtcgttgg gcaggctgcc gggggcgacc
     2581 agctcgatct ggctgcgcag cttggtgacg tcgcgcacgg cgtcggccag gcgctcggcc
     2641 aggccgggca cgttgacgga ttcgacccga agcgtcatct ggtcgttggc catggccccg
     2701 gtgaccacca ggcgggcacg cgccacctcg gggaagcgcc gcaccacctc ggccacctgg
     2761 ccggggtgca cgaacatgcc gcggatcttg gtggtctggt cggcgcgccc catccagccc
     2821 ttgatgcggg tgttggtgcg cccggtgggg caggcaccgg ccagcaccgc cgacagatcg
     2881 ccggtgccga agcggatcag cgggtagtcc gggttgagca cggtgaccac cacctcgccc
     2941 acctcgccct cgggcaccgg atcgccggtg ccggggcgca cgatctcgac gatcacgccc
     3001 tcgtccagca ccaggccctg gcgcgccgcc gtctcgtagg cgatcaggcc ggcatcggcg
     3061 gtggcgtagc tctggtaggc ggtcatgccg cgctcggcga accagtcgcg cagcgacggc
     3121 ggaaagggct cggcgctgac catgcccttg gtcaggctgc gcatgtcggc gccgctttcc
     3181 tgcgccttct cgcacaggat gcgcagaaag ctgggggtgc cgacgtaggc ggtgggcttc
     3241 agctcgccaa tggcctgcag ctgctgctcg gtctggccgg tgccggcggg aaacaccgtg
     3301 cagccaaccg ccagcgcccc ggcctccagg atgaaggcgc cgggcgtcat gtggtagctg
     3361 aaggcgttgt gcaccagatc gccggcgcga aagcccgccg cgtacagcgc gcgcgcggtg
     3421 cgccagtagt ccacggccgt gccctccggc tcgtagatcg ggccggggct ggcgtagacg
     3481 cgccgcatgg ccgcgcccca gcccactgcg gcaaagccgc cgaaggcatc gcgcccggcg
     3541 gcgcggctgg cctgctggcg cgccagcaac tcacccttgc gcgtcaccgg caggcgcgcc
     3601 agcgcggcgc gcgagcctat ggcggcggca tcgacgccgg ccaggatgtc ggcaaaggcc
     3661 gcgctgcgct gctgcgcatg ggcgacgtgc gccggcagcg cggccatcag cgccgcctcg
     3721 cgcgcggcgg ggtcacgggt ttccaacgcg tcgaagaatt cggtcatggt gaatgctctt
     3781 taaacaggag ctccttgcgc aggctgagtg ggctcctgaa tctgttttga ttcatatgcg
     3841 ccgacccatc ggggcacgcc agagcgcacg ccgcgccggc acggcgccgc cgaagggggc
     3901 agcgctcagg ccagccagcg cttgcgccgc ttgtagctct tgacgtcctt gaagctcttg
     3961 cgctcgccgc caccgacgcc gaggtagaac tccttgacgt cctcgttgct ggccaggtcg
     4021 ctggccttgc cgtccatcac gatgcggccg ctttccatga tgtagccgta gtcggcgtac
     4081 ttcagcgcca tgttggtgtt ctgctcggcc agcaggaagg tggtgcgctc cttggtgttg
     4141 aggtcgcgca cgatgttgaa cacctcctcg acgatctgcg gcgccaggcc catcgagggc
     4201 tcgtccagca gcaccatgtc ggggttggtc atgagcgcgc ggccgatggc gcacatctgc
     4261 tgctcgccac ccgaggtgta ggccgcctga ctggtgcggc gcgtcttcag gcgcgggaag
     4321 tagttgtaga ccttctccag gttggcggcg atctcgccct tgtcgctgcg cgtgtaggcg
     4381 ccggtgagca ggttttcctc gatcgtcagg tgggcaaagc agtggcgccc ctccatcacc
     4441 tgcaccacgc cgcgcttgac caggtcggcc gggctgaggt tctcgatgcg ctcgcccttg
     4501 tattcgatcg tgcccttggt gacctcgccg cgctcgccct tgagcaggtt ggagacggcg
     4561 cgcagcgtgg tggtcttgcc ggcgccgttg ccacccagga tggccacgat gccgctgcgc
     4621 ggcaccgtca gcgacacgcc cttgagcacc aggatcacgt ggttgtagat gacctcgatg
     4681 ccgttgacca ccagcagggg cgctggggcg tcggcggcga cgggcgtggc ggtggacatg
     4741 ggcgggtact ccgggaatcg atcgggacga aacgcatgac gcctggcgcg cattccgccc
     4801 gccacgcggg cggaatgcgt caggccggcc tcagttgcag ctgcgcacct tgaggttctt
     4861 ctccttggcg tacttctcgc cgtactcctt gaccagcggg tcgatcatgc tcttgtcggc
     4921 ctgataccaa tccgacacca ccttccagcc gccgccatcc cactgcgcca cgcgcgtcca
     4981 gtcggtgccc atgtggttgt cgcagctggt cttgaccgcg cgcatgatct tgccgaagcc
     5041 cagctcgttc aggcgttcct gcgtcaggtt caggttctcc agaccccagc gcacctgctc
     5101 ggaggtcatg accttgccct tgccgaactt ctcctgcgcg gcacgcacgg cctcgacctg
     5161 cagcatggaa atcatcatgc cgcgcgtgtg ggccatggcg cccagttcgc tggcgtcctt
     5221 ggccgtgccc tgacccttgt cgtacagctg ggccttgacg tcatcgtgca ccttgtcgcg
     5281 ctcggccgtg ttgtggatgg tcatgacgtt gtagcccttg gcacctgcgc cgatgtcctt
     5341 cacgtcgtgg tcggagccgg cccaccagat gccgtagatc ttgtcgcggg cgtagttggt
     5401 ggcctgggcc tcgcgcaggg cggccggcgt catcacgccc gccgaccaca gcagcacgta
     5461 gtcggggcgc tgctggcgca cctgcagcca ggtggatttc tgctccacgc cgggcggcgt
     5521 gacgggcagc agcagcagct cgaagccatc cttggcggcg cgcttttgca gcagcgggat
     5581 cggctccttg ccatagggcg agtcgtggta gaccagcgcg atcttcttgc ccttgaggtt
     5641 gcccttttcc ttcttcagga tgtcctgcac catcgcatcg gcggccgtcc agtaggtgcc
     5701 catcagcggg aagttccact cgaacacgcg cccatcggcc gactgcgaca ggccgtagcc
     5761 ggtggtgacg atggtggcct tgtcgcccgg cgccttgtcg ctgacggcga aggtgatgcc
     5821 ggtggactgc gtgtcgaagc tggctgtgcc gccggggcgc cccttcaggc gctcgtagca
     5881 ctccacgccc ttggcggtgt cgtaggcggt ctcgcactcc tcgaaggcga tcttgacgcc
     5941 gttgatgccc ccgcgggcat tgaccagctt caggtagtcc tgcttgccgt cggcccatgg
     6001 aatgcccagg ggcgcgaact ggccggtgcg gtacaccagc agcgggaaga actgctcctg
     6061 cgcctgcacg gcgggagcgg ccaggccagc aatggcggcg gcggcgagag cgatggcacc
     6121 cagtttcatg gcgagtctcc tttgtgaaac aacagtggtt ggggtgagtg aaacaacgcg
     6181 gcaaatgccc ctgccgacac cggggtaaac acgcagcggc cggccctcaa tgcgggaacg
     6241 gccatacgcg cagcttctgg cgcgcggtgg accacagccg tgccaggcca tggggttcga
     6301 cgatcaggaa gaacacgatc agcgcgccga agatcatgtg ctccaggtag gtggcggtgg
     6361 cggtcgacag cggcaggccc agccagtgcg gcacgtagtt caagaacagc ggcagcagca
     6421 cgaagaaggc ggcaccgaac aggctgccga cgatggagcc cagcccgccg atgatgatca
     6481 tgaacagcag ctggaacgag cggtcgatgc tgaacgcggc cggctcccag gcccccaggt
     6541 ggacgaagcc ccacagcgcg ccagccacgc cgacgatgaa gctgctgacg ccgaacgcgc
     6601 tcagcttggc gtacatcggt cggatgccga tcaccgccgc ggccacgtcc atgtcgcgga
     6661 tcgccatcca ctcgcgaccg atggcgctgc gcaccaggtt cttggccgcc agcgccagta
     6721 cgcacaggat ggccaggcac agcaggtatt tctccatggg ggtgtcgatc ttccagccgg
     6781 cgatcgacag cgggccggcg ctgaccgacc cggacgagga gttgttggtg acccacttgg
     6841 cgcggctggt gagccagtcc atgaagaact gcgccgccag cgtggccacc gccaggtaca
     6901 ggccgcggat gcgcaagctg ggcaggccga acaccatgcc gaacaccatg gccaccagcc
     6961 cgccgcccag cagcgagacc agcagcggca tgccgtcaaa gcgcacctgg aagttgaacg
     7021 ccgcgtaggc ccccaccgcc atgaaggcgc cggtgcccag cgagatctgg ccgcagtagc
     7081 cgaccaggat gttcaggccc agcgcgccca gcgccatgat cagaaacggg atcagaacgg
     7141 cctggaacac gtagtcgctg gccagcagcg gcaccgccac gaaggccagc gccagcagca
     7201 gcagcacggc gacgcggtcc tgcaggatcg ggaagatctg ctgatcggcg cggtaggagg
     7261 tcttgaactg accgttttcc ctgtagaaca tgacagtccg tccccttaca cccggtcaat
     7321 gattttttcg ccgaacagcc cctgcggacg caccagcagc agcgccagcg ccaggccata
     7381 ggcaaaccag ttctcgatgc cgccgccgat catcgggccg aggtacacct ccgacagctt
     7441 ctcgcccacg ccgatgatca gcccgccgat gatggcgccg ggcagcgagg tcagcccgcc
     7501 caggatcacc accggcaggg ccttgagcgc caccagcgac agcgagtact gcacgcccag
     7561 cttgctgccc cagatgatgc ccaccaccag cgccaccacg ccggccaccg accagacgat
     7621 cacccacatg cgcgccagcg gaatgccgat ggactgcgcc gcctggtggt cgtcggccac
     7681 ggcgcgcagc gcgcggccgg tcttggtgaa ctggaagaac accgtcagcg ccgccaccag
     7741 cgcggcggcg atgccggcgg catagaaatc ctccaggctg accagcacgc cgccttcgaa
     7801 catgccttcc agaatgaaca ccgggtcctt gggcatgcca acgttgatgc tgtagaccgc
     7861 gctgccgaaa atcatgggcc cgaaaccatc cagcagatag gcgatgccca gggtggccat
     7921 cagcagcgtg atgggctcct ggttgaccag atggcgcagc gccagccgct cgatcagcca
     7981 ggccacgcca accatgatga ggatgctgac gacgatggcc agcagcacgc tcagcacgcc
     8041 cggcgccatg cccagccagc ccggaatcca ctcggaaaag cgcgccatcg ccagcgccgc
     8101 gaacagcacc atcgcgccct gcgcgaagtt gaacacgccc gaggccttga agatcagcac
     8161 gaagcccagc gccaccagcg cgtacagcat gcccgtcatc aggccgccga acagtgtttc
     8221 gagaaagaac cccatgatct tgcgtccccg tgttcagtgg ctggtgccca ggtaggcccg
     8281 gatcacgtct tcgttgccgc gcacctcgtc gggcgtgccg tcgccgatct tcttgccgta
     8341 gtccagcacc accacgcggt cgctgatgtc catcaccacg cccatgtcgt gctcgatgag
     8401 cacgatggtg gtgccgaact cgtcgttgac gtccaggatg aagcggctca tgtcctgctt
     8461 ttcctcgacg ttcatgccgg ccatcggctc gtccagcagc agcacctgcg gctccatcgc
     8521 caacgcccgg cccaggtcga cgcgcttttg caggccgtag ggcaactggc ccaccggcgt
     8581 cttgcggtgc gcctggattt ccaggaagtc gatgatgtgc tcgacgaact cgcggtgcgc
     8641 gatctcctcg cgctcggccg ggccccagcg cagcgcctgc agcagcaggt tgcttctgat
     8701 cttcaggttg cgcccggtca tgatgttgtc gatcacgctc atgcccttga acagcgccag
     8761 gttctggaag gtgcgcgcca cgcccatctc ggccacctgg cgcgagttca tgtggctgaa
     8821 ggtctggccg cggaaggtga tgctgccttc ctgcggcgtg tagacgccgt tgatgcagtt
     8881 gagcatcgag ctcttgccgg cgccgttggg gccgatgatg gcgcggatct cgtgctcgcg
     8941 gacgttgaag ctgatgtcgg tcagcgcctt gacgccgcca aagcgcaggc tgatgttctg
     9001 cacgtccagg atcacgtcgc cgatcttctt ggcgccagaa gacgaatcaa actggccttc
     9061 agcgcccgtc tggcgagcgc cggcagctat tttttcagta gcaatcgtgt tcatgccgcg
     9121 ctcctgacgg gcgggtaggt cctggcgtcc aggatcttca gcgtggcggc caccttgccg
     9181 gtgcgcccgt cctcgaactt gacggcggtc tcgatgaact gctcggcgcg gcccgcgtac
     9241 agggcgtcga tcagcacgcc gtacttttcg gcgatgaagc cgcggcgcac cttgcgcgtg
     9301 cgcgtcagtt cgtcgtcgtc cggatccagt tccttgtgca gcaccaggaa gcgcgcgatc
     9361 tgcgtggcct ccatgccttc ctcgccggcc aggtcggcgt tcaccttctc gacgcactcg
     9421 gccatcatct tcagcacctc gggcttgccg gccagatcga catagccggc gtagggcagg
     9481 ccctggcgct cggcccagtt gcccacggcc tcgaagtcga tgttgatgaa ggcacagacc
     9541 tgctcgcgct cgtggccgaa gcacacggct tccttgatct gcgggaagaa cttgagcttg
     9601 ttctcgatgt agttgggcgc gaacatggcg ccgccggcca gcttgcccac gtccttggcg
     9661 cggtcgatga tgcgcagatg gccgtcgcgg tccagcacgc cggcgtcgcc ggtcttgaag
     9721 tagccgttct cgtcgatcac ctcggccgtg gcgtcggggc gcttgtagta ttccttgagc
     9781 accgacacgc ccttgaccag cacctcgccg ctgtcggcga tcttcagttc gatgccgggc
     9841 gcggcctggc ccacgctctc cagcttgacc tggccgttct tctggatgca gacgtaggcg
     9901 caggtttcgg tctggccgta cagctgcttg aggttgacgc cgatggagcg gaagaagcga
     9961 aacagatccg gcccgatggc cgcgcccgcc gtgtaggcca cgcggatgcg cgacaggccc
    10021 atggcgttct tcagcggccc gtacaccagc agcttgccaa cgccgtacag cagccggtca
    10081 cccaagccca cgggcttgcc gtccaggatg tcggagccca cgcgccgcgc cacgcccatg
    10141 aagtaggcgt gcatgcgctg cttgaagcgc gccgcgtcct ccatgcggat cgacaccgag
    10201 gtcagcatgc cctcgaacac ccgcggcggc gcgaagtagt aggtggggcc gatctcgcgc
    10261 atgtcgatgc tgacggtgtc gctggactcg gggcagttga tggtgaagcc ggccaccagc
    10321 cactgcgcca tcgagaacag gaagtcgccc acccaggccg gcggcaggta ggacagcacg
    10381 tcgtccgagg cgttcaggcc gtcggtctgc accccgccct gcgccgaggc gacgaaggcc
    10441 gcgtgcgtct ggcacacgcc cttgggccgg cccgtggtgc ccgaggtgta aaggatcacc
    10501 gccacgtcat cgggctggcc ctgcgccacc agctcggcaa acaattgcgg gtgcgcctgc
    10561 tcgtgctggc ggcccagcgc catcagctca tcgacgctga tcaggcccgg ctggcggtag
    10621 ttgcgcaggc cgcgcgggtc gtcgtagatg atgtgggtca gccccggcac cgcctcctcg
    10681 ccctcggcgt cgcgcagctc cagcagcttg tcgacctgct cctggtcttc ggccagggca
    10741 aagtgcacct cggcgtcgcg cagcacgaag gccatctcgg ccgccaccgc gtcctggtac
    10801 atcggcaccg gcacgccacg cagcgactgg gccaccagga acatcaggta cacgtgcggg
    10861 cggttgttgc ccacgatggc caggttctgc cccggtgtga agcccagcgc cgtcaggccg
    10921 caggccatcc agcgcacttc gtccagcgcg cgctgccagc tccaggtctg ccagatgccg
    10981 tattccttct cgcgcagcgc cggctcaccc gggcgctcgg cggcgtgctt ttgcagcagg
    11041 cggggaaagg tgatcgccat gcggctcgtc tcctgtgcgt ccaaccgtcg atgtcccacc
    11101 agaggcatcg ttgtggccgg atggtacggc cagactttga cgccaagttg tcgtttgaac
    11161 gacaattgag accgaatttc gtctgggttt tccctgaatg ccgctgcgcc ttgccctgca
    11221 tgaccgccgt cgcccgctca ccgaggccga gctggccggc gtgccctggc tgtcgcacct
    11281 caagccggac gaatacgacc gcgccgcgcc cgagctgcgc gtcacgcgcg ccgagccggg
    11341 cgagctgatc tttcgcatgg gccggccggt gacctactgg ttcggcgtcg tcgacggcct
    11401 gctgaagatg agcaccgaaa ccgccgacgg acagaccatc acctattccg gcctcacgcc
    11461 cggcggctgg ttcggtgaag gcacggtgct caagcgcgaa ccctaccgct acaacgtcag
    11521 cgcgctgcgc cgcagcctgg tggccggcct gccggccgac accttccact ggctgctgga
    11581 ccattcgatc ggcttcaacc gcttcatcat gagccagctc aacgagcggc tggggcagtt
    11641 catcggcgcg ctggaggccg accgcacgcc caaccccgag cagcgcgtgg cgcgcagcat
    11701 tgccgcgctg gtcaacccgg tgctgttccc cggcgtgggc gaggtgctgc gcatcaccca
    11761 gcaggagctg gcctatctgg tgggcctgtc gcgccagcgc gtcaacgagg cgctgcacgc
    11821 cctggccgcc cagggcctgc tgcgcatcga gtatggcggc ctgcgcattc tcgacctggc
    11881 cgccctgcgc tcacgtcaat tctaggaaaa tcaggctcca gcgcttgtct gtcgagcgca
    11941 agcagctact actttaatag caatcgagtt accccatgac caccgacacg ccccgctttc
    12001 accgcgcgcc gcggcctgcc gccgccgccc cggcgccgac gacgggcggc gcgatccgcc
    12061 tgaacaagcg cctggccgaa ctgggcctgt gctcgcgccg cgaggccgac gactggatcg
    12121 agcgcggctg ggtgcgcgtc aacggccagc tggcggtgat ggggctgaag gtggggccgc
    12181 aagaccaggt cacggtcgac cgccgcgccg acgccgcgca ggcgcagcgc gtcaccatcc
    12241 tgctgcacaa gccggtgggc tacgtcagcg gccaggccga ggacggccac cagccggcgg
    12301 tgaccctgat ccaggcgcgc agccactgga cgggcgacgc cagccgcacc cgcttcgcgc
    12361 cggcgcagct caaggggctg gcgccggccg ggcggctgga catcgactcc accggcctgc
    12421 tggtgctgac gcaggacggc cgcgtggcgc gccagctgat cggcgagcac tcggatgtcg
    12481 acaaggaata cctggtgcgc gtcagctacg gcgcgctggc cgagaacgtg caggccgcct
    12541 ttccggccgc gcaactggcg cgcctgcgcc atggcctggc cctggacggc cagccgctgc
    12601 ggccggcgct ggtcgactgg cagaaccccg agcaattgcg cttcgtgctg cgtgaaggca
    12661 agaagcgcca gatccgccgc atgtgcgagc tggtcggcct gacggtgacc ggcctcaagc
    12721 gcgtgcgcat cggccgcgtg cgcctgggcc agctgcccgc cgggcaatgg cgctacctgg
    12781 gcgagcacga ggcgttctga gccgccgcgc gccggtgggc gccggcgtct tgaaaacccg
    12841 ggccgacagc cccacgtacg gcgcgatttc ttcgcttccc ttctcttcaa ccgtcactgc
    12901 cttttcccat gagcacccag aaacacgcct tccaggccga agtcgcccag ctgctgcacc
    12961 tggtcacgca ttcgctgtac tccaacaagg aaatcttcct gcgcgagctg atctccaacg
    13021 cctccgacgc ctgcgacaag ctgcgcttcg aggcgctggc cgacggcagc ctgtatggcg
    13081 atcagcccga gctgcgcgtg cgcatcgcct tcgacaagga cgcccggacg ctgagcatcg
    13141 ccgacagcgg catcggcatg agcgaggccg aggccatcga gcacctgggc accatcgcca
    13201 agagcggcac gcgcgagttc atggcccgcc tgtcgggcga ccagaaagcc gatgcgcagc
    13261 tgatcgggca gttcggcgtc ggcttctact ccggcttcat cgtcgccgac cggatcacgg
    13321 tggaaacgcg ccgcgccggt ctgccacccg agcagggcgt gcgctggagc agcggcggca
    13381 gcggcgactt cacggtcgag accatcacgc tgcccgagcg cggcacccgc gtcatcctgc
    13441 acctgcgcga ggacgccctg gagttcgccg acggctggcg catcaaggag ctgatcggca
    13501 agtattccga ccacatcgcc ctgccgatcc agatggaagg cgagacctgg gaggaaggca
    13561 aggaagaggg ccagcccggc cgcatggtca agaccggcga gtgggaaacc gtcaaccagg
    13621 ccaccgcgct gtggacgc
//
LOCUS       DAOOOG010000056        13246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_5155_length_13246_cov_20.578273, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000056 DAOOOG010000000
VERSION     DAOOOG010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13246
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5155_length_13246_cov_20.578273"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PK242_05985"
     CDS             complement(<1..301)
                     /locus_tag="PK242_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HOZ93601.1"
                     /translation="MKALLDFLPILLFFGAYKLYGIYPATAVLMAATCVQMGIIYAID
                     RKLTAMHKATLALILVFGTLTLVLQDERFIKWKPTVLYAAMALALAVALWAYRKNF"
     gene            complement(315..725)
                     /gene="msrB"
                     /locus_tag="PK242_05990"
     CDS             complement(315..725)
                     /gene="msrB"
                     /locus_tag="PK242_05990"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOZ93602.1"
                     /translation="MSHPVQKSDAEWRALLADKGAEPLAYQVTRHAATERPFSGRYER
                     HWQDGSYHCICCGARLFESDTKFDAGCGWPSFWQAALPGAIEERRDTSHGMVRVETLC
                     ARCGAHLGHVFPDGPAPTGLRYCMNSAALDFEPR"
     gene            complement(722..2206)
                     /locus_tag="PK242_05995"
     CDS             complement(722..2206)
                     /locus_tag="PK242_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HOZ93603.1"
                     /translation="MLNRTDSVQAVNLGLTWDNAFERLGEGFFTRLAPLPLRGEPYWV
                     ARSAAMARELGLADDWWQVQGALPAVSGGQPLAGSAPLASVYGGHQFGAWAGQLGDGR
                     AILLGEVRTPGGGSQELQLKGSGLTPYSRMGDGRAVLRSSIREFLCSEAMHALGVPTT
                     RALCVAGSDEPVRRERLETAAVVLRAAPSFIRFGHFEHFCHSGRHEALRTLADFVIAR
                     FYPDCRDASNPYAALLGAVTQRTAALLAQWQALGFMHGVMNTDNMSILGLTIDYGPFQ
                     FMDGFDPARICNHSDHLGRYAYQRQPEIAHWNLFALGQALLPLIRQPDAALAVLQPFP
                     ALFSAELDRRMALKLGLPQASDGSRQLVQALLELLTRERTDWTIFWRRLAQQVAGDLR
                     QPVMDLFHDRAAIDRLLLQYQELLAQSNRAQTADLMLKTNPGLVLRNHLAEQAITLAR
                     QHDFSMVQSLHDALQRPFDEQPEHADWCNFPPDWASQIQISCSS"
     gene            2307..3944
                     /locus_tag="PK242_06000"
     CDS             2307..3944
                     /locus_tag="PK242_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HOZ93604.1"
                     /translation="MLGQMQNHPLLTSMLLEFAERHHGDGEIVSRRVEGDIHRYTYRD
                     LARRSRQLAATLDKAGLQQGDRVATLAWNGYRHLELYFGVTGSGRVVHTLNPRLHPEQ
                     LVWIINHAEDAVLCFDMTFLPLIQAVHAKCPSVKTWVALCEADKLPADSGIPQLVSYE
                     AWIGTSTDQYVWPKFDENSAAGLCYTSGTTGNPKGALYSHRSTVLHAYGGALPDALNL
                     SARDSMMPVVPMFHVNAWGLPYVAALTGCKVVFPGPAMDGKSIYELMEAEGVSMAAGV
                     PTIWQMLLGHVQGNKLKFSTLNRTVIGGSACPPAMIDTFREEFGVDVLHAWGMTETSP
                     LGSVSTLKNKHLKQPREQQLAIRVKQGRAICGVDMRIVGDDGNEQPWDGKTFGDLQVR
                     GPWIIESYFKGETPLVADEHGHRWFPTGDVATIDADGYMQITDRSKDVIKSGGEWISS
                     IDIENIAMAHPAIAMAACIGMPHPKWDERPIVAVVRKPGAEVTREELLAFYEGKTAKW
                     QIPDDVVFVDAIPLGATGKMLKTRLRELLKDYKLPTA"
     gene            4058..5296
                     /locus_tag="PK242_06005"
     CDS             4058..5296
                     /locus_tag="PK242_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOZ93605.1"
                     /translation="MKFVWKSVVTASALLLGGAALAQQGEVVKIVRIDPLTGLMGPVG
                     TNQDKSYKFFAEKFSGKGNPAGVTFEFRTIDNKLSPTESLNALKSALDSGVRYIAQGN
                     GSSVTAALLDAVNKHNERNPGKEVLLLNDASVDPDFTNSKCSYWHFRFDADTSMKMEA
                     ITTFMKNKPDIKKVYLLNQNYSHGHQVVKYAKEGLAKKRPDVQIVGEDLHPLAQVRDF
                     APYIAKIKASGADTVITGNWGSDLSLLVKAANEAGYEGKFFTYYAGVTGTPSALGQGA
                     AGRVYQVSYLHSNMGGQMQQWMNEFKAKHNDDFYTGSIPRIYAMLGAAMAQAKSTDPL
                     KVAAALEGLKVNTYNGEIEMRRLDHQLQQPLYVSVWQKTDAKHTYSPENTGMTFVPQA
                     EYPNYVSSTPTSCQMKRPGK"
     gene            5385..6407
                     /locus_tag="PK242_06010"
     CDS             5385..6407
                     /locus_tag="PK242_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ93606.1"
                     /translation="MEFFVISLLNGLSYGLLLFMLSSGLTLIFSMMGVLNFAHASFYM
                     LGAYLGYSIARVTGFWVALLLAPLVVGALGALFERLALRRVHRFGHVPELLITFGLSY
                     VILELVQLIWGRLALEFHPPELLKGPAFTIIKDAAGSLGLTWGAAPAQACQAAGTVCS
                     PFPATRAFMMLSAVVMLVSVWLLLTRTRIGLVIQAALTHPQAVETLGHNVPRVFMLVF
                     GAGCALAGLAGVVGGMTFLTEPAMAATVGSIIFVVVVVGGMGSLAGAFVASLIIGVIQ
                     TFAVALDHSLGSVLAQWGMTLSPTLQGNTLFKMTISQVAPILPYLFLVLILIFRPRGL
                     LGTREG"
     gene            6414..7700
                     /locus_tag="PK242_06015"
     CDS             6414..7700
                     /locus_tag="PK242_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ93607.1"
                     /translation="MTASSRSSASRARWLTWSGFALLLIVAPLVLSSGLSITILSQIG
                     IAIIVCLSYNMLLGQGGMLSFGHAVYSGLGSYLAIHALNAASAGRLPIPVSLIPLVGG
                     LAGLLFAVLLGYVTTKKSGTTFAMITLGVGELVFAMSLMVPEFFGGEGGVSGNRVMGQ
                     PFMGISFGPPIQVYYLIAFYTFLSVAGMYAFTRTPLGRMLNAVRDNPERVEFVGYNAQ
                     HVRYLAFIVAGFFAGVAGGLGALNFEIVTAEVVGAARSGAYLLFTFLGGATFFFGPII
                     GAVLMVLAFVLFSELTKAWLLYLGLIFMFMVMYAPGGIASLIMMNVRVARFGRLGGLI
                     PHYVVLTLAAIVTLAGASALIEMMYQVQLEGSDAALHFLGVTLQPAQTLHWVLAAAVM
                     LAGGALFEWARRRFARQWGSVQEHIEARIKQLEASA"
     gene            7697..8470
                     /locus_tag="PK242_06020"
     CDS             7697..8470
                     /locus_tag="PK242_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ93608.1"
                     /translation="MSVPALQLKDVRKSFGKTEIIRGVSMSVQPGERVGVIGPNGAGK
                     STLFNLISGRFAPSSGEILLKGQRIDGRKPYEINRQGLSRSFQITNIFPKLSVFENLR
                     CGVLWSLGYRYTFLRFLASLDDANQRAEQLMRSIHLERKRDTLAMNLTYAEQRALEIG
                     ITIAGGADVILLDEPTAGMSRSETRHFIELIKEVTVGKTLLTVEHDMGVVFGLADKIA
                     VVVYGEVLAFDTPEAVRANARVQEAYLGSVVAQQQEQGH"
     gene            8473..9168
                     /locus_tag="PK242_06025"
     CDS             8473..9168
                     /locus_tag="PK242_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ93609.1"
                     /translation="MLSVRDLHAFYGKSHVLHGVHFDVQPGEIVALLGRNGSGRSTTA
                     KAVMGMVQAQGDVRFKDQPLLGMKPFQIAHRGLGYVPESRDIFPTLTVQQNLLLGQKG
                     AGKGSRWTFDDMYAMFPRLKEREHTEAGVLSGGEQQMLTLCRTLMGDPELIIIDEPTE
                     GLAPKIVELVGEYLKKLKERGVSVLLIEQKLTIAMTISDRVLVMGHGSIVFEGSPAAL
                     RADAAVRKEWLEV"
     gene            9466..11577
                     /locus_tag="PK242_06030"
     CDS             9466..11577
                     /locus_tag="PK242_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOZ93610.1"
                     /translation="MSADYQLHGDVAVITLNNPPVNGLGYATRVGITNGLERANADPA
                     VKAIVITGAGKAFSGGADIREFGTPKALQEPNLLSVILAIEHSAKPVVAAVHSVAMGG
                     GLELALGCHYRVAAPGAQIALPEVKLGLLPGAGGTQRLPRVLGVETALNMIVKGDPVS
                     SEVLASLPGQKLFDQMAASAESLLQEALAFAAQAAARQAAGEALPLVRQLPCKHPQGD
                     AYFQFARNMVKGMAKNFPAPAKCVDAVQAATKNKFDAGMAFERAIFVELMNTPESAAL
                     RHLFLAERAASKIPDVGDDVKPRAIQKVAVIGAGTMGGGISMNFLNAGIPVTILETKQ
                     EALDRGLATIRKNYEAQVKKGKLKQDKYEQRMALLTPTLSYDDLRDADLVIEAVFEEM
                     GVKEQVFKKLDEVVKPGAILASNTSTLDVDKIAAFTQRPQDVVGMHFFSPANVMKLLE
                     VVRGAKTAKDVLATVMQIAKKIRKTAVVSGVCDGFIGNRMIEQYSRQAGFLLEEGATP
                     QQVDKAVEKFGFAMGPFRMGDLAGNDIGWAIRKRRYVEKPDMKYSKTADLLCELGRYG
                     QKTGAGWYDYQPGKRDAIPSKLVEDMIAKHRQEQGITPRKISDEEIVQRLVYALVNEG
                     ARILEDGIASKASDIDMVYLTGYGFPIWRGGPMKYAEQVGLFNVVESMKRFARNPLDD
                     ARFWQPAPLLSKLVAEGRNFS"
     gene            11636..12811
                     /locus_tag="PK242_06035"
     CDS             11636..12811
                     /locus_tag="PK242_06035"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOZ93611.1"
                     /translation="MTSAVIVSTARTPLAKSWKGAFNMTHGATLGGHAVQHAIARAGI
                     DAAQVEDVIMGCANPEGATGMNIARQIALMAGCPISVSGMTVNRFCSSGLQTIALAAQ
                     RIIAGEGTTFVAGGVESISCVQQEMNMHMIQDPALLARKPEIYWSMLQTAEQVAKRYG
                     IGRDVMDEYGAASQQKACAAQAAGKFDDEIAPITVTMGVADPVRGLSTRQVTCSKDEG
                     TREGTTKEGISGIRPALPGGLVSAGNASQFSDGAGACVLMHESEASKQGLKPIGRFLG
                     FAVAGCEPDEMGIGPVYAVPKVLQRLGLKVGDIDLWELNEAFAVQVLYCRDKLGIPAD
                     RLNVNGGAIALGHPYGVSGQRLTGHALIEGKRRGAKRVCVTMCIGGGMGAAGVFEVL"
BASE COUNT         2329 a         4304 c         4390 g         2223 t
ORIGIN      
        1 ggaagttctt gcggtaggcc cacagcgcca ccgccagcgc cagcgccatg gcggcataca
       61 ggaccgtcgg cttccatttg atgaagcgct cgtcctgcag caccagggtc agcgtgccga
      121 agaccaggat caaggccagg gtggccttgt gcatggccgt gagcttcctg tcgatggcgt
      181 agatgatgcc catctgcacg caggtggcgg ccatcagcac ggcggtggcc gggtagatgc
      241 cgtacagctt gtaggcaccg aagaacagca ggatgggcag gaagtcgagc agggctttca
      301 tgcaggcgcg cagtttaccg gggctcgaag tccagcgcgg ccgagttcat gcagtagcgc
      361 agcccggtgg gtgccgggcc gtcggggaac acatggccca ggtgggcgcc gcaccgggcg
      421 cacagggttt cgacgcgcac catgccgtgg ctggtgtcgc gccgctcctc gatggcgccg
      481 ggcagggcgg cctgccagaa gctgggccag ccgcagccgg cgtcgaactt ggtgtcggac
      541 tcgaacagcc tggcgccgca gcagatgcag tggtagctgc catcctgcca atggcgctcg
      601 tacctgcccg aaaacgggcg ttcggtggcg gcgtggcgcg tgacctggta ggccagtggc
      661 tcggccccct tgtcggcaag cagggcacgc cactcggcgt cggatttctg aacgggatgg
      721 ctcatgagga acagctgatc tggatctgtg atgcccagtc gggcggaaag ttgcaccagt
      781 cggcgtgctc gggctgctcg tcgaacgggc gctgcagcgc gtcgtgcaag gactgcacca
      841 tgctgaagtc gtgctgccgg gccagcgtaa tggcctgctc ggccaggtgg ttgcgcagca
      901 ccaggccggg attggttttc agcatcaaat cggcagtttg cgccctattg gattgcgcca
      961 gaagctcctg atattgtagc aaaaggcgat cgatggcggc ccgatcgtga aacaaatcca
     1021 tcaccggctg gcgcaggtca ccggccacct gctgcgccag gcggcgccag aagatggtcc
     1081 agtcggtgcg ctcgcgcgtc agcagctcga gcagcgcctg caccagctgg cgcgagccgt
     1141 cgctggcctg cggcaggccg agcttgagcg ccatgcggcg atccagctcg gcgctgaaca
     1201 gcgccgggaa gggctgcagc accgccagcg ccgcatcggg ctgccgaatc agcggcagca
     1261 gcgcctgtcc gagggcaaac aggttccagt gggcgatctc gggctggcgc tggtaggcgt
     1321 agcgccccag gtggtcggaa tggttgcaga tgcgggccgg gtcgaagccg tccatgaact
     1381 gaaacggccc gtagtcgatg gtcaggccca ggatgctcat gttgtccgtg ttcatcacgc
     1441 catgcatgaa gcccagcgcc tgccactgtg ccagcagcgc cgcggtgcgc tgcgtcaccg
     1501 cgcccagcag cgccgcgtag gggttgctgg cgtcgcggca gtcggggtag aagcgcgcga
     1561 tgacgaagtc cgccagcgtg cgcagcgctt cgtgccgacc gctgtggcaa aagtgctcga
     1621 aatgcccgaa gcggatgaag ctgggggcgg cgcgcagcac cacggcggcg gtttccaggc
     1681 gttcacgtcg caccggctcg tccgagccgg cgacgcacag cgcccgcgtg gtcggcacgc
     1741 ccagcgcatg catggcctcg ctgcacagga actcgcggat cgacgagcgc agcaccgcgc
     1801 ggccgtcgcc catgcgcgag tagggcgtca ggccactgcc tttgagctgc agctcctgcg
     1861 agccaccgcc cggcgtgcgc acttcgccca gcaggatggc gcggccgtcg cccagttgcc
     1921 cggcccaggc gccgaactgg tggccgccgt agacgctggc cagcggcgcg ctgccggcca
     1981 gcggttggcc gccgctgacg gccggcaagg cgccctggac ctgccaccag tcgtcggcca
     2041 ggccgagttc acgcgccatg gcggcgctgc gggccaccca gtacggctcg ccacgcagcg
     2101 gcagcggcgc caggcgggtg aaaaaaccct cgcccaggcg ttcgaaggcg ttgtcccagg
     2161 tcaggcccag attgaccgct tggacggagt cggtcctgtt cagcatcaag ccattgtcac
     2221 ttgcgtggca cgagcgtgca gaaaccccag ggtggtgaat tagtattgca tctgctatcg
     2281 caaccgctcg cacaaagagg agaaccatgc tgggccagat gcagaaccac cccttgctga
     2341 cttccatgct gctggagttc gccgagcgcc accacggcga cggcgaaatc gtttcccgca
     2401 gggtcgaggg cgacatccat cgctacacct accgcgacct ggccaggcgc tcgcgccagt
     2461 tggccgccac gctcgacaaa gcggggctcc agcagggcga ccgcgtggcc acgctggcgt
     2521 ggaacggcta ccggcacctg gagctctact tcggggtcac cggctccggg cgcgtcgttc
     2581 acacgctcaa tccgcgcctg catccggagc agctggtgtg gatcatcaac cacgccgaag
     2641 acgccgtgct gtgcttcgac atgacgttcc tgcccctgat ccaggccgtg catgccaagt
     2701 gcccgtcggt caagacctgg gtcgccctgt gcgaggccga caagctgccg gccgacagcg
     2761 gcattcccca gctggtcagc tacgaggcct ggatcggcac ctcgaccgac cagtatgtct
     2821 ggcccaagtt cgacgaaaac agcgccgccg gcctgtgcta caccagcggc accaccggca
     2881 accccaaggg cgccctgtac agccaccgct cgaccgtgct gcacgcctac ggcggcgcac
     2941 tgcccgatgc gctcaacctg tcggcgcgcg actcgatgat gccggtggtg cccatgttcc
     3001 acgtcaacgc ctggggcctg ccctatgtcg cggcgttgac cggctgcaag gtggtgtttc
     3061 cggggccggc gatggacggc aagtcgatct acgagctgat ggaggccgag ggcgtcagca
     3121 tggccgccgg cgtgcccacc atctggcaga tgctgctggg gcatgtgcag ggcaacaagc
     3181 tcaagttctc caccctcaac cgcaccgtga tcggcggctc ggcctgccca ccggccatga
     3241 tcgacacctt ccgcgaggag ttcggcgtcg acgtgctgca tgcctggggc atgaccgaaa
     3301 ccagcccgct gggttcggtc agcacgctca agaacaagca cctcaagcag ccgcgcgagc
     3361 agcagctggc cattcgcgtc aagcaggggc gcgccatctg cggcgtggac atgcgcatcg
     3421 tcggcgacga cggcaacgag cagccctggg acggcaagac cttcggtgac ctgcaggtgc
     3481 gtggcccgtg gatcatcgag tcctacttca agggcgagac cccactggtg gccgatgagc
     3541 acggccaccg ctggttcccc accggcgatg tggcgaccat cgatgccgac ggctacatgc
     3601 agatcaccga ccgcagcaag gacgtcatca agtccggcgg tgagtggatc agctcgatcg
     3661 acatcgaaaa catcgccatg gcgcaccccg cgatcgccat ggccgcctgc atcggcatgc
     3721 ctcaccccaa gtgggacgag cgcccgatcg tcgccgtggt caggaagccc ggcgccgagg
     3781 tgacgcgcga ggagctgctg gcgttctacg aaggcaagac cgccaagtgg cagattccgg
     3841 acgacgtggt gttcgtcgac gccattccgc tgggcgccac cggcaagatg ctcaagaccc
     3901 gcctgcgcga gctgctcaag gactacaagc tgccgaccgc ctgacgcacg gcgctacccg
     3961 cctggcactt gcgcgacaac gggttggggc aaacccggct gcagtgtgcc ggcagcaaag
     4021 ctacgcttac ggccgtttca atccaaggag acatcagatg aagttcgttt ggaaatccgt
     4081 cgtgaccgcc tcggcgctgc tgctgggcgg cgccgccctg gcgcagcagg gcgaggtggt
     4141 gaagatcgtg cgcatcgacc cgctgaccgg cctgatgggt ccggtgggca ccaaccagga
     4201 caagagttac aaattcttcg ccgaaaagtt cagcggcaag ggcaacccgg ccggcgtgac
     4261 cttcgagttc cgcaccatcg acaacaagct gagcccgacc gaaagcctga acgcgctgaa
     4321 gtcggcgctc gactccggcg tgcgctacat cgctcagggc aatggctcct cggtcacggc
     4381 ggccctgctg gacgccgtca acaagcacaa cgagcgcaac ccgggcaagg aagtgctgct
     4441 gctgaacgac gcctccgtcg acccggattt caccaacagc aaatgcagct actggcactt
     4501 ccgcttcgac gccgacactt cgatgaagat ggaggccatc accaccttca tgaagaacaa
     4561 gcccgacatc aagaaggtgt acctgctgaa ccagaactac tcgcacggtc accaggtggt
     4621 caagtacgcc aaggaaggtc tggccaagaa gcgccccgac gtgcagatcg tcggcgaaga
     4681 tctgcacccg ctggcgcagg tgcgcgactt cgcgccctac atcgcgaaga tcaaggcttc
     4741 gggcgccgac acggtgatca ccggcaactg gggttccgac ctgtcgctgc tggtcaaggc
     4801 cgccaacgaa gccggctacg agggcaagtt cttcacctac tacgccggcg tcacgggcac
     4861 accgtccgct ttggggcagg gtgctgccgg gcgggtgtat caggtctcct acctccattc
     4921 caacatgggt ggccagatgc agcagtggat gaacgagttc aaggccaagc acaacgatga
     4981 cttctacacc ggctcgatcc cacgcatcta cgccatgctg ggcgctgcca tggcgcaggc
     5041 caagtccacc gatcctttga aggtggcggc ggccctggaa gggctcaagg tcaacaccta
     5101 caacggcgag atcgagatgc ggcggctgga tcaccagctg cagcaacccc tgtatgtctc
     5161 ggtctggcag aagaccgacg ccaagcacac ctacagcccg gagaacaccg gcatgacctt
     5221 cgtgcctcag gccgaatacc ccaactacgt ctccagcacg ccgaccagct gccagatgaa
     5281 gcggcccggc aagtgacgac acaggcggcc tgaagtcgcc gccgtgactg cgaaggcccg
     5341 aacgggttgc cgtctcgggc cttcattttg gagaatcgcc ggccatggaa tttttcgtca
     5401 tctcgctgct caacggcctg agctacggtc tgctgctgtt catgctcagc tccggtctga
     5461 cgctgatctt cagcatgatg ggggtgctca atttcgccca cgccagcttc tacatgctgg
     5521 gcgcctatct gggctacagc atcgcgcgcg tcaccggctt ctgggtggca ctgctgctgg
     5581 cgccgctggt ggtcggcgcc ctgggcgcgc tgttcgagcg cctggcgctg cggcgggtgc
     5641 accgcttcgg ccatgtgccg gagctgctga tcaccttcgg gctgtcctat gtgatcctcg
     5701 aactggtgca actgatctgg ggccggctgg cgctcgaatt ccatccgccc gagctgctga
     5761 aaggccccgc attcaccatc atcaaggacg ctgccggcag cctgggcctg acctgggggg
     5821 cggcgccggc gcaagcctgt caggcggcgg gcacggtctg ttcgccgttc ccggccacgc
     5881 gcgccttcat gatgctgtcg gcggtggtga tgctggtgtc ggtgtggctg ctgctgacac
     5941 gcacccgcat cggcctggtg atccaggccg cgttgacgca cccgcaggcg gtggagaccc
     6001 tgggccacaa cgtgccgcgc gtgttcatgc tggtgtttgg tgcgggctgc gcgctggccg
     6061 gcctggccgg tgtggtgggg ggcatgacct ttctcaccga gccggcgatg gcggccaccg
     6121 tgggctcgat catcttcgtg gtggtggtgg tgggcggcat gggctcgctg gccggtgcgt
     6181 tcgtggcttc gctgatcatc ggtgtgattc agacctttgc cgttgccctg gaccattcgc
     6241 tcggctccgt gctggcgcaa tggggcatga cgctcagtcc aacgctgcag ggcaacacgc
     6301 tgttcaagat gacgatctcg caggtggcgc ccatcctgcc gtatctgttc ctggtgctga
     6361 tcctgatctt caggccccgg gggttgttgg gcacacgcga gggttgacgc accatgaccg
     6421 cttcgtcacg ttcctcggcc agccgggcgc gctggctcac ctggtccggt tttgcgctgc
     6481 tgctgatcgt ggcgccgctg gtgctgtcca gcggcctgtc catcaccatc ctgtcgcaga
     6541 tcggcatcgc catcatcgtc tgcctgtcct acaacatgtt gctggggcag ggcggcatgc
     6601 tcagcttcgg ccacgcggtg tattcgggcc tgggttccta tctggccatc cacgcgctca
     6661 atgcagcctc ggcgggccgc ctgccgattc cggtgtcgct gattccgctg gtgggaggtc
     6721 tggcgggcct gctgtttgcg gtgctgctgg gctatgtgac caccaagaag tccggcacca
     6781 ccttcgccat gatcacgctg ggtgtgggcg aactggtgtt cgccatgtcg ctgatggtgc
     6841 cggagttctt cggtggcgag ggcggcgtgt cgggcaatcg ggtgatgggg cagccgttca
     6901 tgggcatcag tttcggtcca ccgattcagg tctattacct gatcgcgttc tacaccttcc
     6961 tgagcgtcgc cggcatgtat gccttcacgc gcacaccgct ggggcgcatg ctcaacgccg
     7021 tacgtgacaa cccggaacgg gtcgagttcg tcggctacaa cgcccagcac gtgcgctatc
     7081 tggcgttcat cgtggccggc ttctttgccg gtgtcgccgg tggcctgggg gcgctcaact
     7141 tcgagatcgt caccgccgag gtggtgggcg ccgcgcgctc gggcgcctac ctgctgttca
     7201 ccttcctggg tggcgccacc ttcttcttcg gccccatcat cggcgcggtg ctgatggtgc
     7261 tggccttcgt gctgttttcc gaactcacca aggcctggct gctgtacctg ggcctgatct
     7321 tcatgttcat ggtgatgtat gccccgggcg gcatcgccag cctgatcatg atgaacgtgc
     7381 gggtggcgcg cttcggacgg ctgggggggc tgatcccgca ctatgtggtg ctgacgctgg
     7441 ccgccatcgt gacgctggca ggcgcctcgg ccttgatcga aatgatgtat caggtgcaac
     7501 ttgaaggctc cgacgcggcg ctgcactttc tgggggtgac gctgcagccg gcgcagaccc
     7561 tgcactgggt gctggctgcc gcggtgatgc tggcgggtgg tgcgctgttc gagtgggcgc
     7621 gccgccgctt cgcgcgccag tggggctcgg tccaggaaca catcgaagcg cgcatcaagc
     7681 aactggaggc ttcggcatga gcgtgcccgc cctgcaactg aaggacgtgc gcaagagttt
     7741 cggcaagacc gagatcattc gcggcgtcag catgagcgtg cagcctggtg agcgcgtggg
     7801 cgtgatcggg cccaacggtg cgggcaagtc gacgctgttc aacctgatca gcggccgctt
     7861 tgcgcccagc agcggcgaga tcctgctcaa agggcagcgc atcgacggtc gcaagcccta
     7921 cgagatcaac cgccagggcc tgtcgcgcag cttccagatc accaacatct ttcccaagct
     7981 gagcgtgttc gagaacctgc gctgcggtgt gttgtggagc ctgggttacc gttacacgtt
     8041 cctgcgcttt ctggccagcc tggacgacgc caaccagcgc gccgagcagc tcatgcgcag
     8101 catccacctg gagcgcaagc gtgacaccct ggcaatgaac ctgacctacg ccgagcaacg
     8161 cgcgctggag atcggcatca ccatcgccgg cggggccgac gtgatcctgc tggacgagcc
     8221 caccgccggc atgagccgca gcgaaacgcg ccatttcatc gaactgatca aggaggtgac
     8281 ggtgggcaag accttgctca cggtcgagca cgacatgggg gtggtgttcg gcctggccga
     8341 caagatcgcg gtggtggtgt acggcgaggt gctggcgttc gacacccccg aagcggtgcg
     8401 cgccaacgcg cgcgtgcagg aagcctatct gggctcggtg gtggcgcagc agcaggaaca
     8461 ggggcattga cgatgttgag cgtgcgcgac cttcatgcct tctacggcaa gagccatgtg
     8521 ctgcatggcg tgcatttcga tgtgcaaccc ggcgagatcg tggcgctgct ggggcgcaac
     8581 ggttcgggcc gatcgaccac ggccaaggcc gtgatgggca tggtgcaggc gcagggtgat
     8641 gtgcgtttca aggatcagcc gctgctgggc atgaagccgt tccagatcgc ccaccggggc
     8701 ctgggctatg tgccagaaag ccgcgacatt ttccccaccc tgacggtgca gcagaacctg
     8761 ctgctgggcc agaagggcgc aggcaagggc agtcgctgga cgttcgacga catgtacgcc
     8821 atgtttccgc gcctgaagga gcgcgagcac accgaagccg gcgtgctgtc gggcggcgag
     8881 cagcagatgc tgaccctgtg ccgcaccctg atgggcgacc ctgaactcat catcatcgac
     8941 gagcccaccg aaggcctggc gcccaagatc gtcgaactgg tgggcgaata cctgaagaag
     9001 ctgaaggagc gcggcgtgtc cgtgctgctg atcgagcaga agctgaccat cgccatgacg
     9061 atctccgatc gcgtgctggt gatggggcac ggcagcatcg tgttcgaagg ctcgcctgct
     9121 gcactgcgcg ccgacgcggc ggtgcgcaag gagtggctgg aagtgtgaag ctcgggcgag
     9181 ggcagggtgg ggctccacgc tatcggttca atagctgttg gcgcatgctc caccttgatt
     9241 ctgaagcctt tcgccatgat tttccttgcg cagcaagcgc cgacagctat cgaacctgaa
     9301 gcgcgctgcg ccttcgtctc tcctgtgaca acccggtcac gtcaccatgg cgcatactat
     9361 ccacacccgt ctgatcgcgg tcgtctccgg gcccgccagg agcgccgaag gccgcgacgt
     9421 caccatctca cattcccgca atccgcgcac acctgaggag cccgcatgag cgctgactac
     9481 cagctgcacg gcgatgtcgc cgtcattacc ctgaacaacc cgccggtcaa cggcctgggc
     9541 tacgccacgc gggtgggcat caccaacgga ctggagcgtg ccaacgccga cccggcggtc
     9601 aaggccatcg tcatcaccgg cgccggcaag gcgttttccg gcggtgccga catccgtgaa
     9661 ttcggcacgc ccaaggcctt gcaggagccc aacctgcttt cggtcatcct ggccatcgaa
     9721 cactcggcca agccggtggt cgccgctgtc cacagcgtgg ccatgggcgg gggcctggag
     9781 ctggccctgg gctgccacta ccgcgtggcg gcaccgggcg cgcagattgc cttgcccgag
     9841 gtcaagctgg gcctgctgcc gggcgccggc ggcacccagc gcctgccgcg cgtgctgggg
     9901 gtggagaccg cgctcaacat gatcgtcaag ggcgacccgg tgagcagcga agtgctggcc
     9961 agcctgcctg ggcagaagct gttcgatcag atggccgcca gcgccgaatc gctgctgcag
    10021 gaggccctgg cgtttgccgc tcaggccgcc gcccgtcagg cggcgggcga agcgctgccg
    10081 ctggtgcgcc agttgccgtg caagcacccg cagggcgacg cgtacttcca gttcgcgcgc
    10141 aacatggtca agggcatggc caagaacttc ccggcacccg ccaaatgtgt cgacgccgtg
    10201 caggccgcca ccaagaacaa gttcgatgcc ggcatggcgt tcgagcgggc gatcttcgtg
    10261 gaactgatga acacccctga aagcgctgcc ctgcgccacc tgttcctggc cgagcgtgcg
    10321 gccagcaaga ttcccgacgt gggcgatgac gtcaaaccgc gcgccatcca gaaagtggcg
    10381 gtgatcggcg ccggcaccat gggcggcggc atatcgatga acttcctcaa cgccggcatt
    10441 cccgtcacca ttctggagac caagcaggaa gcgctggacc gcggcctggc caccatccgc
    10501 aagaactacg aggcgcaggt caagaagggc aaactcaagc aggacaagta cgagcagcgc
    10561 atggccctgc tgacccccac gctgagctac gacgacctcc gcgacgccga cctggtgatc
    10621 gaggccgtgt tcgaggagat gggcgtcaag gagcaggtgt tcaaaaagct cgacgaagtg
    10681 gtcaaacccg gcgccatcct ggcgtccaac acctccacgc tcgacgtcga caagatcgcc
    10741 gccttcaccc agcgcccgca ggacgtggtg ggcatgcact tcttcagccc ggccaacgtg
    10801 atgaagctgc tcgaagtggt gcgcggcgcc aagaccgcca aggacgtgct ggccacggtg
    10861 atgcagatcg ccaagaagat caggaagacc gcggtggtct ccggcgtgtg cgacggtttc
    10921 atcggtaatc gcatgatcga gcagtacagc cgccaggccg gcttcctgct ggaagagggc
    10981 gccaccccgc aacaggtcga caaggcggtc gagaagttcg gctttgccat gggtccgttc
    11041 cgcatgggcg atctggccgg caacgacatc ggctgggcca tccgcaagcg ccgctacgtc
    11101 gaaaagcccg acatgaagta cagcaagacc gccgatttgc tgtgcgaact gggccgctac
    11161 ggccaaaaaa ccggcgccgg ctggtacgac taccagcccg gcaagcgtga cgccattccc
    11221 agcaaactgg tcgaggacat gatcgccaag caccgccagg agcagggcat cacaccgcgc
    11281 aagatcagcg acgaagagat cgtgcagcgt ctggtgtacg cgctggtcaa cgaaggcgca
    11341 cgcattttgg aagacggcat cgccagcaag gccagcgaca tcgacatggt gtatctgacc
    11401 ggctatggct ttccgatctg gcgcggcggc ccgatgaaat acgccgagca ggtgggcctg
    11461 ttcaacgtgg tggaaagcat gaagcgcttt gcgcgcaacc cgctggacga cgcccgcttc
    11521 tggcaacccg cgccgctgct gagcaagctc gtcgccgaag gcaggaattt cagctgattt
    11581 cgcggcccag ccattgatca gtcagcgcca acagctatcg aatcaggagt aattcatgac
    11641 ctctgccgtg atcgtatcca ccgcccgcac cccgcttgcc aagagctgga agggcgcttt
    11701 caacatgacc catggcgcca ccctgggcgg ccatgcggtg caacacgcca tcgcccgcgc
    11761 gggcatcgac gccgcgcagg tggaagacgt catcatgggc tgcgccaacc ccgaaggcgc
    11821 caccggcatg aacatcgcgc gccagatcgc gctgatggcc ggttgcccga tcagcgtctc
    11881 cggcatgacg gtgaaccgtt tctgctcgtc gggcctgcag accatcgccc tggcggcgca
    11941 gcgcatcatc gccggagaag gcaccacctt cgtcgccggc ggcgtggaga gcatctcctg
    12001 tgtccagcag gaaatgaaca tgcacatgat ccaggatccg gcgctgctcg ccaggaagcc
    12061 cgagatctac tggagcatgc tgcaaaccgc cgagcaggtg gccaagcgct acggcatcgg
    12121 ccgcgacgtc atggacgaat acggcgccgc cagccagcag aaggcctgtg ccgcgcaggc
    12181 cgcgggcaag ttcgacgacg aaatcgcccc catcaccgtc accatgggtg tggccgatcc
    12241 ggtgcgcggc ctgagcacgc gacaggtcac ctgcagcaag gacgaaggca cgcgcgaggg
    12301 caccaccaag gaaggcatca gcggcatcag gccggcgctg cccggtggtc tggtttcggc
    12361 cggcaacgcc agccagttca gcgacggcgc tggcgcctgc gtgctgatgc acgaaagcga
    12421 agccagcaaa cagggtctga aaccgatcgg ccgctttctg gggtttgccg tggccggttg
    12481 cgaacccgac gaaatgggca tcggcccggt ctatgccgtg cccaaggtgc tgcaacgtct
    12541 ggggttgaag gtcggcgaca tcgacctgtg ggagctcaac gaagccttcg ccgtgcaggt
    12601 gctgtactgc cgcgacaagt tgggcattcc cgccgatcgg ctgaacgtca acggcggcgc
    12661 gattgcgctg ggccatccgt acggcgtcag cggccagcgc ctgaccgggc acgccctgat
    12721 cgaaggcaag cgccgcggcg ccaagcgcgt gtgcgtgacc atgtgcattg gcggcggcat
    12781 gggtgcggcc ggcgtgttcg aagtgctgtg atttgaccgg aaactggcgc tcccgttgcg
    12841 ccgccagcgc cccgcaccgg acgatcggtg cggggcgctt tcgtgttcgc caccgttcca
    12901 gcagcggtgc ggcggctcaa tccggacgac agcgaaagtc tgacgcgagt ccaccaccac
    12961 gatggtttaa cctagaaacg gcagttgacc gctggtcaag ttgaccgctg gtcaacttcg
    13021 ggcagaccgc atcgatgcgg ggtctggcgg ctgcgatggc cttcacctcc tgggtgagca
    13081 cgctacgcac ccacccgcac cgcgctcgcc gcgccatgcg gcgttgctcc ttcttgcggg
    13141 caccagcccg cggcgtcgtc gcgtcttgcc tggcacgccg atcacggcac gggcgggggc
    13201 gtccaacggc cgctttaaat cgtaatgaag acgatgttta ttatgt
//
LOCUS       DAOOOG010000057        13223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_5175_length_13223_cov_25.774985, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000057 DAOOOG010000000
VERSION     DAOOOG010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13223
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5175_length_13223_cov_25.774985"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PK242_06040"
     CDS             <1..621
                     /locus_tag="PK242_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOZ93612.1"
                     /translation="HVARTGDIGLFKIVSEGGVAAGVRRIEAITGEHALGYLQHLEAS
                     LHGVAATLRAPAGEVQARLGQVLDQVKALEKEIAQLKGKLASSQGDELVNQAVEVKGL
                     KVLAARLDGADAKTLRDTLDKLKDKLGSAAIVLATVDGDKVQLAAGVTRDAVGRVKAG
                     ELVSHVARQVGGKGGGKPDMAMAGGTDAKALPAALASVQAWVAERV"
     gene            complement(642..1385)
                     /locus_tag="PK242_06045"
     CDS             complement(642..1385)
                     /locus_tag="PK242_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93613.1"
                     /translation="MHRKTFLIAAACLGSCAAGALAADAGTGLTSRASRAVQAPFDIV
                     HTRISTQGQVATFRMTVSGRAGASRPARSGKLAGSKVFSYVWPTSLDPAVVGFEPKAG
                     ILAFAVTAHPDFDDTPLFDENGDGRLDNDGNVWHSHWVVLQPDAACGKDALKVVDIPA
                     GSKPRLPKTWPGLPILIDSPGWSPTLKGRTLEVRVPFDDIGAVESGAFDGVTAALRVN
                     ASVHSPLLCVVNVFKVASGDLSLPGKVNR"
     gene            1600..2478
                     /locus_tag="PK242_06050"
     CDS             1600..2478
                     /locus_tag="PK242_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOZ93614.1"
                     /translation="MQTAPPFDRLSGILERFRLRARLHHAGALCGLHHFDAGEGHGYL
                     HVLRRGQLAVSHPGAREVPQALHFEQPTLLLYPRPFTHHFHNPPDEGADFTCARLDFE
                     GGAEHPLARALPPLIALPLARVEGLGLALELLFAETDRPRCGHRLLVDRIFEVVVIQL
                     LRWLLDHPQEAGVRPGFVTGMAHPGLARALIAMHDAPGEGWTLARLAACAGMSRTAFA
                     VTFRDVVGQTPADYLSDWRVALAQTRLRAGQPVKAMAEELGYAHPSALSRAFGARVGM
                     SPRQWLAQVSAGDAPR"
     gene            complement(2490..4127)
                     /locus_tag="PK242_06055"
     CDS             complement(2490..4127)
                     /locus_tag="PK242_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOZ93615.1"
                     /translation="MACLRIVVLVSTLALGAPSQAQSPPSTQASDPRAMGWMQGFPPP
                     ADKTIRFTDPDYFSFPKLRWTVCHFRELMPTAGVDRGPGAASALPQAPEPGIDALRFT
                     PLGGQASMSWGEAFDANHTDGLLVLHHGRVVYERYAGCLGPHTLHGAMSLTKSITGLL
                     GEVLVAEGALDENARVGTLIPQLQDSAFGDASVRQVLDMTTALQFSEDYADPNAEVWQ
                     HAQAGSTLPPPAGYTGPRSYYESLQQIRKSGEHGQVFGYKTPNADALGWLLARTTGKP
                     LNELLAERIWRRIGAEREAFYTVDAIGTPFAGGGFNVTLRDLARLGQLILQQGEWQGV
                     QLLPSAAIERIQRGGERAPFARAGYAQLPGWSYRAMWWHSGDAHGAFSARGVHGQTLW
                     IDPVADVVIARLASHPVAANAANDPTSLPAWRAVTKHLMAQDQTPLLGGQWVIEDVAG
                     AGVIDRTRAFLRFLADGRLVGHATCNGLRGHHTSGARGRLRLRLTGITRKRCAPALMH
                     QEERLLALLPRVTRYRIDDSGALWLYTAAGKTLTARR"
     gene            4594..5079
                     /locus_tag="PK242_06060"
     CDS             4594..5079
                     /locus_tag="PK242_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOZ93616.1"
                     /translation="MPPPNAAAARPAPELNVSQWFNTDTAPTLAALRGQVVMLHAFQM
                     LCPACVRLATPQAQQVHEALGRQGLVVLGLHTVFEHHEAMRPVSLAAYIHENRLRFPI
                     GVDRADDLHDLPLTMRAYGMQGTPTLLLIDRQGRLRQHSFGHVPDLVLGAAIGTLLGE
                     P"
     gene            complement(5519..6466)
                     /locus_tag="PK242_06065"
     CDS             complement(5519..6466)
                     /locus_tag="PK242_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ93617.1"
                     /translation="MSQRPVQALALPDDLVVLQRDWLSSNNLLCLGEQAAIVDTGHVK
                     HAAQTVRRVRAVLGTQPLATIAHTHLHSDHCGGTGALQAAWPTATTWVPEPSLDAVQR
                     WDEDALSFDGTGQRCARFGADRALVPGQPVRLGAHDWQVHAAPGHDSLAVLLFEPRHG
                     ILVSGDALWEHGVGVIFPHIDGSAGFEPFNETLDVIERLAPEIVIPGHGAPFGRAGGA
                     IDAALGRARERIATFQAQPAQHALYAAKVLIKYQMLDVDAMTHADFERWLNAAPSLHL
                     LHRQHRPDLDWPSWLAHVLAPMFGKGVLQRDATHVRDGN"
     gene            6665..7099
                     /locus_tag="PK242_06070"
     CDS             6665..7099
                     /locus_tag="PK242_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93618.1"
                     /translation="MRHVGLVGRARCVATRDAHGHVGVLLMIETPQRIPHEARSEIQA
                     YFRRKLMEFGELKGRPFHLLLRDRDELSQTQRDPVRSSSSRIAAIVAAANVGVDAVTL
                     AEQVADRREQVRQRLSERRQARQESAYVPLAPVTDVAPLAEV"
     gene            complement(7381..8322)
                     /locus_tag="PK242_06075"
     CDS             complement(7381..8322)
                     /locus_tag="PK242_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOZ93619.1"
                     /translation="MARAALPPLPPRDGVGPSCVVVPGAGWPTVLDFLAERLPALTRQ
                     AWQQRLQGGQVLDAQGRPVAAELACRSGLRLYYYRGWADEPALEGAPEQIVFQDDWLV
                     VADKPHFMPVTPAGRFVQRSLLVRLKRRLGLPGLSPIHRIDRETAGLVVLAVQPHTRA
                     AYQGLFRDRAVHKLYEAVAPDRAELAQPRTHRSRLEPDPQRFFISREQPGAPNSETHV
                     ARAAVLGKHALYHLRPVSGQRHQLRLHLCALGAPILGDAFYPHVLRGPDAPDDRTRPL
                     QLLARELAFDDPYTGQRRQFTSGLELAAAAQRWPLTS"
     gene            complement(8333..9094)
                     /gene="trmB"
                     /locus_tag="PK242_06080"
     CDS             complement(8333..9094)
                     /gene="trmB"
                     /locus_tag="PK242_06080"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOZ93620.1"
                     /translation="MAHPPDAAGPAAQAGADVTHPRAIRSFVTRAGRTTRGQARALAE
                     LGPRFVLPYDQNVPDLIASCASQTSAGGQKYQKAVLEIGFGMGHATAHIAALMPDTLF
                     IGCEVHEAGVGALLKLIGEQGLDNIRIVPHDAVQVLRHMIAPGTLDGIHIFFPDPWHK
                     TRHHKRRLIQPPFVRELAERLRPGGQLHLATDWQPYAEQMLAVLSAEPLLVNTADGYA
                     PRPDYRPQTKFERRGLRLGHGVWDLVFRRRLAEMA"
     gene            complement(9094..9999)
                     /locus_tag="PK242_06085"
     CDS             complement(9094..9999)
                     /locus_tag="PK242_06085"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOZ93621.1"
                     /translation="MSSSLHPMLNVAIRAARAAGAIINRAALDVEAVRISQKQVNDFV
                     TEVDHASEDAIIDTLLNAYPGHGIHAEESGTTRGNPQSDHVWIIDPLDGTTNFIHGFP
                     VYCVSIALAVKGRIEQAVIYDPTRNDLFTATRGRGAFLNDRRIRVSKRTRLPECLIST
                     GFPYRKGDDFQGYLRMMADVMQRTAGLRRPGAAALDLAYVAAGHTDGFFETGLKPWDL
                     AAGSLLITEAGGLIGNFSGEAGFLEQGECLAGNPRVFAQLVQLLRPHSRFDGAPQPSG
                     EQRAPLSLRKTDEAAQPATTPPLQP"
     gene            10197..10625
                     /locus_tag="PK242_06090"
     CDS             10197..10625
                     /locus_tag="PK242_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOZ93622.1"
                     /translation="MANPIVRSRLFDDFFNDFAPGFFVKPLHGDPLPTAAQIRIDVKE
                     NNGTYTVEAEMPGVGKDDIHVSIDGSVVTVRAEIKQQDSQSSGERVLRSERYYGAVAR
                     SFQLPQDIDQSAAKARYDNGVLTLTLPKKQVSGSQRLTIE"
     gene            complement(10665..12062)
                     /locus_tag="PK242_06095"
     CDS             complement(10665..12062)
                     /locus_tag="PK242_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOZ93623.1"
                     /translation="MSSPARRAFLRRLGQLGLAGSAAPWAINLAAIGEAAAFSADGYK
                     ALVCVFLYGGNDNGNTLIPYDSASHADYATARQSLAIARANLAGTVLSGSDLPAGREY
                     ALAPQLAPLKPLFDAKRLALQLNVGPLRVPTTLEQYRARSVPLPPKLFSHNDQQSVWQ
                     SSAAEGAVRGWGGRLGDLALAGNEGSALFTCLSVTGNAVFLSGEHALAYQIGAGGAVA
                     IKGVTQDDGMYGAQACRQALRTLITEPRAQVLESELNRITARSLDSHAVLAGALATSV
                     GQFDAVLPDQVDGRRRTLNQQLKMVARLIAARGQLGPRRQVFLVSLGGFDNHDNLMNR
                     HPALLTELGAGLAGFQQALDALGVGPQVTTFTASDFGRTLVSNGDGSDHGWGSHHFVL
                     GGAVRGGAFYGQAPQAVLNGPEDVGQGRLLPTTAVDQYAATLARWFGVSDTELPLVAP
                     AIGQFGTRDLGFLGA"
     gene            complement(12083..>13223)
                     /locus_tag="PK242_06100"
     CDS             complement(12083..>13223)
                     /locus_tag="PK242_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HOZ93624.1"
                     /translation="ITLNPAMGAYLNTRGNKKANASGRVPDENYAREVMQLFTIGLYE
                     LNPDGTLRGGQPQETYGPADVSQLAQVFTGYDLDAASGAYKTDPAPFRRPMKLNPAQH
                     AGDSVTVLGRSIAAGDGTARLRQALDILFEHPNVGPFIGRQLIQRLVTSAPSPAYVGR
                     VAAAFNDNGAGVRGDLRAVTRAVLLDPEARQDPKLNGPAWGKLREPMIRFVQWARSFG
                     ATSSDGKWILWDLSDPSTALGQSPLRSPSVFNFFRPGYVPPGTALAERGQPAPEFQLT
                     NENSVAGYLNYMQGAIAWGAWGSKTSKVWVERYTHEMALVQDAAALVERLNLLLAAGQ
                     LSPATVATIRDAIATLKPDSDWGRNQRVWSAIALVMACPEYLVQK"
BASE COUNT         1916 a         4457 c         4873 g         1977 t
ORIGIN      
        1 cacgtggcgc gcaccggcga catcggcctg ttcaagatcg tcagcgaagg cggcgtggcc
       61 gccggcgtgc gccgcatcga ggccatcacg ggcgagcacg cgctgggcta cctgcagcac
      121 ctggaagcca gcctgcacgg cgtcgccgcc accttgcgcg cacccgctgg cgaagtgcag
      181 gcgcgcctgg gccaggtgct cgaccaggtc aaggcgctgg aaaaggaaat cgcccagctc
      241 aagggcaagc tggcctcgag ccagggcgac gaactggtga accaggcggt cgaggtcaag
      301 ggcctgaagg tgctggccgc ccggctcgac ggggccgacg ccaaaaccct gcgcgacacc
      361 ctggacaagc tcaaggacaa gctgggcagc gccgccatcg tgctggccac cgtcgatggc
      421 gacaaggtgc agctggccgc cggcgtcacc cgggacgccg tgggccgcgt gaaggcgggc
      481 gagctggtca gccatgtggc ccggcaggtg ggcggcaagg gcggcggcaa gcccgacatg
      541 gccatggccg gcggcaccga cgccaaggcg ctgcccgcgg cgctcgcttc agtccaggcc
      601 tgggtggccg agcgcgtctg acgcgccagc cggccgccgc cttaccggtt gaccttgccg
      661 ggcagcgaca ggtcgcccga cgccaccttg aacacattca ccacgcacag cagcgggctg
      721 tgcacgctgg cgttgacgcg cagcgccgcg gtcacgccgt cgaaggcacc ggattcgacg
      781 gcgccgatgt cgtcgaacgg cacccgcacc tccagcgtgc ggcccttcag cgtggggctc
      841 cagcccgggc tgtcgatcag gatcggcagg cccggccagg tcttgggcag gcgcggcttg
      901 ctgccggccg ggatgtccac caccttcagc gcgtccttgc cgcaggcggc atcgggttgc
      961 agcaccaccc agtgcgaatg ccagacgttg ccgtcgttgt ccagccggcc atcgccgttt
     1021 tcgtcgaaca gcggcgtgtc gtcgaagtcg gggtgggcgg tgaccgcgaa cgccagaatg
     1081 ccggccttgg gctcgaagcc gaccacggcc gggtccaggc tggtcggcca gacataggaa
     1141 aacaccttgc tgccggccag cttgccggat cgggcagggc ggctggcgcc ggcccggccc
     1201 gacaccgtca tgcggaacgt ggccacctgt ccctgggtcg agatgcgggt gtggacgata
     1261 tcgaacggag cctgcacggc ccggctggct cggctggtca ggccagtgcc ggcatcggcc
     1321 gccagggcgc cggcggcgca gctgcccagg caggcggcgg cgatcagaaa ggttttgcgg
     1381 tgcatcacga tcggctcctt tgaacgaagg ggatggattc agtatggggg catgtgttgt
     1441 acgataagca cccaatcgtt cacaaaacat acctgatcgt tcagactgga aacggcagct
     1501 gggtgcgcgc acgtcatggg tgaacgtcat cgaagccatg aacgccagcg cccatgcggc
     1561 tggcacgaag atcgcaagcg gtcagctgca cttggttcaa tgcaaacggc cccacccttc
     1621 gaccgcctgt cgggcatcct ggagcgcttt cgcctgcggg cgcgcctgca ccacgccggc
     1681 gcgctgtgtg gtctgcacca cttcgatgcg ggcgaagggc atggctacct gcacgtgctg
     1741 cgccgcggcc agctggcggt cagccatcct ggcgcgcgcg aggtgccgca ggcgctgcac
     1801 ttcgagcagc ccacgctgct gctgtacccg cgcccgttca cgcaccactt tcacaacccg
     1861 ccggatgagg gcgcggactt cacctgtgcc cggctggact tcgaaggcgg ggccgagcac
     1921 ccgctggcgc gcgcactgcc gccgctgatc gcgctgccgc tggcgcgcgt ggaaggcctg
     1981 gggctggcgc tggagctgtt gtttgccgaa accgatcgcc cacgctgcgg ccaccggctg
     2041 ctggtggacc ggatcttcga ggtggtggtg atccagttgc tgcgctggct gctcgaccac
     2101 ccgcaggagg ccggcgtgcg ccccggcttt gtcaccggca tggcgcaccc gggtctggcg
     2161 cgcgccctga tcgccatgca cgatgcgccg ggcgagggct ggacgctggc gcgcctggcc
     2221 gcgtgcgccg gcatgtcgcg cacggcgttt gccgtcacgt ttcgcgacgt ggtcggtcag
     2281 accccggccg actacctgag cgactggcgc gtggcactgg cgcagacccg gctgcgcgcc
     2341 ggtcagccgg tcaaggccat ggccgaagag ctgggctacg cccacccgtc ggcgctgtcg
     2401 cgcgcctttg gcgccagggt ggggatgtcg ccgcggcagt ggctggcgca ggtgtcggcg
     2461 ggtgatgcgc cgcgctgatc gcggccggtt cagcgccgcg cggtaagcgt cttgccggcg
     2521 gccgtgtaca gccacagggc gccgctgtcg tcgatgcggt agcgggtgac gcgcggcagc
     2581 agcgccagca gccgctcctc ctggtgcatc agcgccggcg cgcagcgctt gcgggtgatg
     2641 cccgtcaggc gcagccgcaa ccttccccgg gcgccgctgg tgtggtggcc gcgcagaccg
     2701 ttgcaggtgg catggccgac caggcgcccg tcggccagaa agcgcaggaa ggcgcgcgtg
     2761 cggtcgatca cgccggcgcc ggcgacgtcc tcgatcaccc attggccgcc cagcaggggg
     2821 gtctggtcct gcgccatcag gtgcttggtc acggcgcgcc aggccggcag cgaggtcggg
     2881 tcgttggcgg cgttggcggc caccgggtgc gaggccagac gcgcgatcac cacgtcggcc
     2941 accggatcga tccacagcgt ctgaccgtgc acgccgcgcg cgctgaaggc gccgtgcgcg
     3001 tcgccgctgt gccaccacat ggcgcggtag ctccagcccg gcagctgcgc gtagccggcg
     3061 cgggcgaacg gcgcgcgttc gccgccgcgc tggatgcgct cgatcgccgc cgacggcagc
     3121 agctgcacgc cctgccattc gccctgctgc aggatcagct gacccaggcg cgccagatcg
     3181 cgcagcgtga cgttgaagcc accgccggca aatggcgtgc cgatggcgtc gacggtgtag
     3241 aaggcttcgc gttcggcgcc gatgcggcgc cagatgcgct cggccagcag ttcgttcagt
     3301 ggcttgccgg tggtgcgcgc cagcagccag cccagggcgt cggcattcgg ggtcttgtag
     3361 ccaaaaacct ggccgtgctc gccgctcttg cggatctgct gcagcgattc gtagtagctg
     3421 cgcgggccgg tgtagcccgc cggcggcggc agcgtgctgc cggcctgggc atgctgccag
     3481 acttcggcgt tcgggtcggc gtagtcctcg gaaaactgca gcgccgtcgt catgtccagc
     3541 acctggcgca cgctggcgtc gccgaaggcg ctgtcctgca gctgcgggat cagggtgccg
     3601 acgcgggcgt tttcgtccag cgcgccttcg gccaccaaca cttcgcccag caggccggtg
     3661 atcgacttgg tgagcgacat ggcgccgtgc agcgtgtgcg ggcccagaca gccggcgtag
     3721 cgctcgtaga cgacgcggcc gtggtgcagc accagcaggc cgtcggtgtg gttggcgtca
     3781 aaggcttcgc cccagctcat cgacgcctgg ccgcccagcg gggtgaagcg cagtgcgtcg
     3841 atacccggct ccggcgcctg cggcagcgcg ctggcggcac cggggccgcg gtcgacaccg
     3901 gcggtgggca tcagctcgcg aaagtggcac accgtccagc gcagcttggg gaagctgaaa
     3961 tagtccgggt cggtgaaacg gatggtcttg tcggccggcg gcggaaagcc ctgcatccag
     4021 cccatggcgc gcgggtcgct cgcctgggtg gacggcggcg actgagcctg gctcggggcg
     4081 ccgagggcga gtgtgctgac cagcacaacg atgcgcagac atgccacggt caagcccacg
     4141 gcccatcggt catggaagtc gtcaccagtc attttggaac gcgcgtcggt ggttggagag
     4201 cgctggcaag ggccatggta gccgcagcgc gtgtgccatc gtggccagcg tggataccct
     4261 gcggcgcgcg tcggcggggc gtgcctggtg cagtgtttcg cgctgtcagg cacttataga
     4321 agccagaaaa aggcttgcgg caaggcgcgg ctcgcagccc atgcggcggc cttggcaagc
     4381 ggcgcaacgc cgccgcaagc ctttttccgg cttcccgcag ggcgagccgc ccgggggcca
     4441 tctgcgcggc gcgtggcctt gacatggctc caccatgcct gcggccacgc gccgcgcagc
     4501 tggccccttg gcgactcgct aaaagtgcct ggcagcgcga agcactgcac tacctgccca
     4561 taatggcctg cccaccaccc tggaagctct gccatgccac cgcccaacgc cgctgccgcc
     4621 cgtccggcgc ccgagttgaa cgtgagccag tggttcaaca ccgacaccgc gccgacgctg
     4681 gccgcgctgc gcggtcaggt ggtgatgctg catgcgttcc agatgctgtg cccggcctgc
     4741 gtgcgcctgg cgacgccgca ggcgcagcag gtgcatgaag cgttgggccg tcagggcctg
     4801 gtggtgctgg gtctgcacac ggtgttcgaa caccacgagg ccatgcggcc ggtgtcgctg
     4861 gcggcctaca tccacgaaaa ccgcctgcgt tttccgattg gcgtggatcg cgcggacgac
     4921 ctccacgatc tgccgctcac catgcgtgcc tacggcatgc aaggcacgcc cacgctgctg
     4981 ctgatcgacc gtcagggacg cttgcgccag cacagctttg gccatgtgcc ggacctggtg
     5041 ctgggcgcgg ccatcggcac cttgctgggc gaaccctgag ccagcgcccg gcgaaggcgc
     5101 gcgcgatcgc agcgactcgc gtccacgatc gacgtgttgc atgctctacc atcaggtgaa
     5161 ggtgtgcgaa gcccggtgaa gaagcggtcg tcacgaagta cacgagccgt caactgcccg
     5221 ttcccagatt caatccaaaa gcggcagttg gacgcgcccg ccggtgccgt gatcggcgtg
     5281 ccaggcaaga cgctacgacg ccgcgggctc gtgcccgcaa gaaggagcaa cgccgcatgg
     5341 cgcggcgagc gcggtgcggg tgggtgcgca gcgcgctcac ccaggaggtg aaggccatcg
     5401 cagccgccag accccgcatc gatgcggtct gcccgaggtt ggcaagcggt caactgccgt
     5461 ttctaggttc aatggcctgg atttgttcgg taggcgcatt cagccgtcga ttgcaggctc
     5521 agttgccgtc ccgtacgtgg gtggcgtcgc gctgcagcac gcccttgccg aacatgggcg
     5581 ccagcacatg cgccagccag ctgggccagt ccaggtcggg ccggtgctgg cggtgcagca
     5641 ggtgcagcga gggcgcggcg ttcagccagc gctcgaagtc ggcgtgcgtc atggcgtcga
     5701 cgtccagcat ctggtacttg atcagcacct tggcggcgta cagggcgtgc tgcgccggct
     5761 gtgcctggaa cgtggcgatg cgctcgcgcg cgcggcccag tgccgcgtcg atggcgccac
     5821 ccgcgcggcc gaacggggcg ccgtggccgg ggatgacgat ctcgggggcc agccgctcga
     5881 tcacgtccag cgtctcgttg aagggctcga aaccggccga accgtcgatg tgcggaaaga
     5941 tcacgcccac gccgtgttcc cacagcgcgt cacccgacac caggatgccg tggcgcggct
     6001 cgaacagcag caccgccagg ctgtcgtgcc ccggcgcggc gtgcacctgc cagtcgtggg
     6061 cgcccaggcg caccggctgg cccggcacca gcgcccgatc ggcgccgaag cgggcgcagc
     6121 gctggccggt gccgtcgaag ctcagggcgt cttcgtccca gcgctggacc gcgtcgagcg
     6181 agggctcggg cacccaggtg gtggcggtcg gccaggccgc ctgcagcgcc ccggtgccac
     6241 cgcagtggtc gctgtggagg tgggtgtggg cgatggttgc cagcggctgc gtgcccagca
     6301 ccgcgcgcac ccgccgcacg gtctgcgccg cgtgcttgac gtgcccggtg tcgacgatgg
     6361 cggcctgctc gcccaggcaa agcaggttgt tggacgacag ccagtcacgc tgcagcacga
     6421 ccagatcgtc gggcaaggcc agcgcttgca caggtctttg tgacatacaa acaaaagtta
     6481 tcgtataact gcgccagccc cccaattcgg ggggggcggt ttgcgcaggg tgtctgcgga
     6541 gccggtttgc ccgcaagggt gctctgggta cggggtttcg atgcagttta tccaacgtct
     6601 tcggcgccgc ctgcgcgcgc cgtcgcttga cctcaggcgt ctgcggggcg ccagtcggtt
     6661 tttgatgcgc catgtcggcc tggtggggcg cgcacgctgc gtcgccacgc gcgatgcgca
     6721 tggccacgtg ggtgtgctgc tgatgatcga gacgccgcaa cgcattccgc acgaggcgcg
     6781 aagcgaaatc caggcgtact ttcgtcgcaa gctgatggag tttggcgagc tcaagggccg
     6841 ccccttccac ctgctgttgc gcgatcgcga cgaactgagc cagacccagc gtgacccggt
     6901 gcgcagcagt tcgtcgcgca ttgcggccat cgtggcggcc gccaacgtgg gtgtcgatgc
     6961 cgtcacgctg gccgaacagg tggccgacag gcgcgaacag gtgcgccagc gcctgagcga
     7021 gcgccgccag gcgcgccagg aaagcgccta cgtgccgctg gcgccggtga ccgacgtcgc
     7081 cccgctggcc gaagtgtgag aggctgtgag ttctcccctg cgcccgcctt gcacgccaag
     7141 acacccccgc tcatcgttgc agatgcgcgt cacagcccgc attgtggttg cgttgggtgc
     7201 cttccgaacg gtattccacc aggaaacggc cgttggacgc gcccacccgc gccgtgagcg
     7261 gcatgccagg caagggtgaa ggccgcggcg ccagagttgc ctgcgcgcct tgggacaacg
     7321 cggtcgggcc cagcctctgg ctgggctgcg acgcgcgtca gggacttgtg ctgcgccggc
     7381 ctatgacgtg agcggccagc gctgcgccgc cgccgccagc tccagaccgg aagtgaactg
     7441 gcgccgctgg ccggtgtacg ggtcgtcgaa cgccagctcg cgcgccagca gttgcagcgg
     7501 gcgcgtccgg tcgtccggcg cgtcgggacc gcgcagcacg tgcggataga aggcatcgcc
     7561 caggatcggc gcgcccaggg cgcacaggtg cagccgcagc tggtggcgct ggccgctcac
     7621 cgggcgcagg tggtagagcg cgtgtttgcc cagcacggcg gcgcgcgcca catgggtttc
     7681 gctgttgggc gcgccgggtt gctcgcgcga gatgaagaag cgctgcggat cgggctccag
     7741 ccggctgcgg tgggtgcggg gctgcgccag ctcggcccgg tcgggcgcca ccgcctcgta
     7801 cagcttgtgc accgcgcggt cgcggaacag cccctggtag gcggcgcggg tgtgcggctg
     7861 cacggccaac accaccagcc cggcggtttc gcggtcgatg cggtggatgg ggctgaggcc
     7921 gggcaggccc agccggcgct tcaggcgcac cagcaggctg cgctgcacga agcggccggc
     7981 cggcgtgacc ggcatgaagt gcggtttgtc ggccaccacc agccagtcgt cctgaaacac
     8041 gatttgctcg ggcgcgccct ccagcgcagg ctcgtcggcc cagccgcggt agtagtacag
     8101 acgcaggccg ctgcggcagg ccagctcggc ggccaccggg cgaccctgcg cgtccagcac
     8161 ctgaccgcct tgcaggcgct gctgccaggc ctggcgcgtc agcgccggca ggcgttcggc
     8221 caggaagtcc agcaccgtgg gccagccggc gccgggcacc accacgcagc tggggccgac
     8281 gccgtcgcgc ggcggcagcg ggggcagggc ggcgcgggcc atgtgcgggt gttcacgcca
     8341 tctcggccag ccggcgccgg aacaccaggt cccacacgcc gtggcccagc cgcaggccgc
     8401 ggcgctcgaa cttggtctgc ggccgatagt ccgggcgcgg tgcatagccg tcggcggtgt
     8461 tgaccagcag cggctcggcg ctcagcacgg ccagcatctg ctcggcgtag ggctgccagt
     8521 cggtggccag atgcagctgg ccgcccggcc gcaggcgctc ggccagctcg cgcacgaagg
     8581 gcggctggat caggcggcgc ttgtggtggc gcgtcttgtg ccaggggtcg ggaaagaaga
     8641 tgtggatgcc gtccagcgtg ccgggcgcga tcatgtggcg cagcacctgc acggcgtcgt
     8701 gcggcacgat gcggatgttg tccagaccct gctcaccgat cagcttgagc agcgcgccga
     8761 cgcccgcttc atgcacttcg cagccgatga acagggtgtc cggcatgagc gccgcgatgt
     8821 gcgccgtggc atggcccatg ccaaaaccga tctccagcac cgctttctga tatttttggc
     8881 ctccggcgct tgtctggctt gcgcaggaag ctatcaaatc aggaacattc tggtcgtagg
     8941 gcaggacaaa gcgcggcccc agctcggcca gcgcgcgggc ctggccgcgg gtggtgcggc
     9001 cggcgcgcgt gacgaagctg cggatggcgc gcgggtgcgt cacgtcggcg ccggcctgcg
     9061 ccgccgggcc ggcggcgtcc ggagggtggg ccatcacggc tgcaggggtg gggtggtggc
     9121 gggctgggcc gcttcgtcgg tcttgcgcag gctcaggggc gcgcgctgct cgccgctggg
     9181 ctgcggcgcg ccgtcgaagc gcgaatgcgg gcgcagcagc tgcaccagct gggcgaacac
     9241 gcgcgggttg ccggccaggc attcgccttg ctccagaaag ccggcttcgc cgctgaagtt
     9301 gccgatcaga ccgcccgctt cggtgatcag caggctgccg gcggccaggt cccagggttt
     9361 gaggccggtc tcgaagaaac cgtcggtgtg accggccgcc acgtaggcca ggtcgagcgc
     9421 ggcggcgccg gggcggcgca ggccggcggt gcgctgcatc acgtcggcca tcatgcgcag
     9481 atagccctgg aagtcgtcgc ccttgcggta cggaaagccg gtggagatca ggcactcggg
     9541 caggcgcgtg cgcttgctga cgcggatgcg ccggtcgttc aggaaggcgc cgcgcccgcg
     9601 cgtggcggtg aacaggtcgt tgcgcgtggg gtcgtagatg acggcctgct cgatgcggcc
     9661 cttgaccgcc agcgcgatgc tgacgcagta gaccggaaag ccgtggatga agttggtggt
     9721 gccgtccagc ggatcgatga tccagacatg gtcggactgg gggttgccgc gcgtggtgcc
     9781 cgattcctcg gcatggatgc cgtggccggg gtaggcgttc agcagggtgt cgatgatggc
     9841 gtcttcgctg gcgtgatcga cttcggtgac gaagtcgttg acctgcttct gcgagatgcg
     9901 cacggcctcg acgtcgagcg cggcgcggtt gatgatggcg ccagcggcgc gcgcggcccg
     9961 gatggccacg ttgagcatgg ggtgcaggga agacgacatg ggttgtggga agagcgtggg
    10021 gcccgcggcg cgcgatggcg cggggcaaca ggagacgcat tttcccatgc tttggcgacc
    10081 ggcgccggcg ccgccgccag caccggcgcg gcacccttga aatcgggcgc gccgttcaca
    10141 cctgctgagc aagggaggtt tcaacccttc atcgcatcca ccgaaaggag attcacatgg
    10201 ccaaccccat cgttcgcagc cgactcttcg atgacttctt caacgacttc gcgcccggct
    10261 tcttcgtcaa gccgctgcat ggcgatccgc tgccgacggc ggcgcagatc cgcatcgacg
    10321 tgaaggaaaa caacggcacc tacaccgtcg aggccgaaat gcccggcgtc ggcaaggacg
    10381 acattcacgt ctcgatcgac ggcagcgtgg tgacggtgcg cgccgaaatc aagcagcagg
    10441 acagccagtc cagcggcgag cgcgtgctgc gtagcgagcg ctactacggt gccgtggcgc
    10501 gcagcttcca gctgccccag gacatcgacc agagcgccgc caaggccagg tacgacaacg
    10561 gcgtgctgac gctcaccctg cccaagaagc aggtcagtgg ctcgcagcgc ctgaccatcg
    10621 agtaagcgcc acgccaaccc gccgggctgc gccggcgggt tggcctatgc gcccagaaaa
    10681 cccaggtcgc gcgtgccgaa ctgcccgatg gcgggcgcca ccaggggcag ttcggtgtcg
    10741 ctgacgccga accagcgcgc cagcgtggcg gcgtactggt cgaccgccgt ggtgggcagc
    10801 aggcggccct gaccgacgtc ttccgggccg ttcagcacgg cctgcggcgc ctgaccgtag
    10861 aaggcaccgc cgcgcaccgc gccgcccagc acgaaatggt ggctgcccca gccgtggtcg
    10921 gagccgtcgc cgttggacac cagggtgcgg ccgaagtccg acgccgtgaa ggtggtgacc
    10981 tgtggcccca cgcccagcgc gtccagcgcc tgctgaaacc cggccagacc cgcgcccagc
    11041 tcggtcagca gcgccgggtg ccggttcatc aggttgtcgt ggttgtcgaa gccacccagc
    11101 gagaccagga acacctggcg cctgggcccc agctggccgc gcgcggcgat caggcgcgcc
    11161 accatcttga gctgctggtt gagcgtgcgg cgccgcccgt ccacctgatc gggcaggacg
    11221 gcgtcgaact ggccgacgct ggtggccagc gcccccgcca gcacggcgtg cgagtccagc
    11281 gaacgcgcgg tgatgcggtt gagttcgctc tccagcacct gggcgcgcgg ctcggtgatc
    11341 agcgtgcgca gcgcctggcg gcaggcctgg gcgccgtaca tgccgtcgtc ctgggtcacg
    11401 cccttgatcg ccaccgcccc gccggcgccg atctggtagg ccagagcgtg ctcgcccgac
    11461 aggaacaccg cgttgccggt gaccgacagg caggtgaaca gggcgctgcc ttcgttgccg
    11521 gccagggcca gatcgcccag ccggccaccc cagcccctga ccgcaccttc ggcggccgag
    11581 ctttgccaga ccgattgctg gtcgttgtgc gagaacagct tgggcggcag aggcaccgag
    11641 cgcgcccggt actgctccag cgtggtgggc acgcgcagcg ggccgacgtt gagctgcaac
    11701 gccagccgct tggcgtcgaa cagcggcttg agcggcgcca gctggggtgc cagcgcatat
    11761 tcgcggccgg ccggcaggtc gctgccgctc agcacggtgc cggccaggtt ggcgcgcgcg
    11821 atggcaagcg actgccgcgc cgtggcgtag tcggcgtggc tggcgctgtc gtagggaatc
    11881 agggtgttgc cgttgtcgtt gccgccatac aggaagacgc acaccagggc cttgtagccg
    11941 tcggcgctga aggccgcggc ttcgcctatc gccgccaggt tgatggccca gggtgcggcg
    12001 ctgccggcaa ggccgagctg gccgagccgg cgcaggaagg cgcgccgggc aggggaagac
    12061 atgcggtgga tggccgtggg attcatttct gcaccaggta ttcgggacag gccatcacca
    12121 gcgcgatggc cgaccagacg cgctggttgc ggccccagtc gctgtcgggc ttgagggtgg
    12181 cgatggcgtc gcggatggtg gcgacggtgg cgggcgagag ctggccggcc gccagcagca
    12241 ggttgagccg ctcgaccagc gccgccgcgt cctgcaccag cgccatctcg tgcgtgtagc
    12301 gttcgaccca gaccttgctg gtcttgctgc cccaggcgcc ccaggcgatg gcgccctgca
    12361 tgtagttgag gtagccggcc accgagttct cgttggtgag ctggaactcc ggcgccggct
    12421 ggccgcgctc ggccagcgcg gtgcccggcg gcacgtagcc ggggcggaag aagttgaaca
    12481 ccgaggggct gcgcagcggg ctctggccga gcgcggtgga agggtcggac aggtcccaca
    12541 ggatccactt gccgtcgctg gaggtggcgc cgaagctgcg cgcccactgc acgaagcgga
    12601 tcatgggttc gcgcagcttg ccccaggccg ggccgttgag cttggggtcc tggcgcgcct
    12661 ccggatccag cagcacggcc cgggtgacgg cgcgcaggtc gccgcgcacg ccggcgccgt
    12721 tgtcgttgaa ggccgccgcc acccgcccga cataggcggg gctgggcgcg ctggtgacca
    12781 ggcgctgaat cagctggcgg ccgatgaagg gccccacgtt ggggtgctcg aacaggatgt
    12841 cgagcgcctg gcgcaggcgc gccgtgccgt cgccggcggc gatgctgcgg cccagcacgg
    12901 tcacgctgtc gcccgcgtgc tgggccgggt tgagcttcat cggacggcga aacggcgccg
    12961 gatcggtctt gtaggcaccg ctggcggcgt cgaggtcgta gccggtgaag acctgagcca
    13021 gctggctgac gtcggccggg ccgtaggttt cctgcggctg gccgccgcgc agcgtgccgt
    13081 cggggttgag ctcgtacagg ccgatggtga acagctgcat cacctcgcgc gcgtagttct
    13141 cgtccggcac gcggccgctg gcgttggcct tcttgttgcc gcgcgtgttc aggtaggcgc
    13201 ccatggccgg gttgagggtg atg
//
LOCUS       DAOOOG010000058        13167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_5203_length_13167_cov_24.564979, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000058 DAOOOG010000000
VERSION     DAOOOG010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13167
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5203_length_13167_cov_24.564979"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PK242_06105"
     CDS             <1..609
                     /locus_tag="PK242_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOZ93625.1"
                     /translation="ADRFTTGSSIMPQKKNPDVPELARGKTGRVVGHLMGLITLMKGQ
                     PLAYNKDNQEDKEPLFDTVDTLRDTLRIFVEMIGGQPNPVSGAKEGGISVNPAAMEAA
                     AQKGYATATDLADYLVKKGLPFRDAHETVAHAVKAAQSHACDLSELPLKVLQEFHPAI
                     DKDVYQVLSLRGSLNARATLGGTAPAQVQTQIARHRARLALG"
     gene            complement(716..1720)
                     /locus_tag="PK242_06110"
     CDS             complement(716..1720)
                     /locus_tag="PK242_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ93626.1"
                     /translation="MTRAVVTGGAGFIGSHLCEALLIQGVQVTCVDDLSSGSSDNVAH
                     LLAVPGFAFVPGDVVDPALWQRLPAAQRVFHLASPASPVDYQAAPVQTIRTNVLGTLN
                     ALDFARAHGARMLLTSTSEVYGDPQVHPQTEAYRGHVNPVGPRSCYDEGKRCAEALTV
                     AHARQHGTQVRLARLFNTYGPRLRPQDGRVVSTFITSALRAEPLPLHGDGRQTRSFCY
                     VSDTVRGLLALMAQERVTGPINLGNPQEVSMHALAAMVLRLTGSNAGVRTLPAREEDP
                     RCRQPDISQAQRWLEWQPEVPLVQGLAQTIEWFRAQGAVGADRLPCGSQPVVDLAANT
                     "
     gene            complement(1717..2580)
                     /locus_tag="PK242_06115"
     CDS             complement(1717..2580)
                     /locus_tag="PK242_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ93627.1"
                     /translation="MVRCINLHNPLLTKGERIVTVSESSGCAAGLRVAVVTPYFKESP
                     ATLQRCLTSVRAQTYPQVVHYMVADGFPQNALLTQWPGVRHIQLPNGHANFGCTPRGV
                     GAQCALADGFDVVCFLDADNLIEPEHVATVVETVAKAQAAGVALDAVFSLRYLFLPGH
                     EHLRLVAPREDVGSGFVDTSCISLTRSAGFLWGAWCQIPPSLTPICDRVMCWLMQHHR
                     LKVAWTGQRTVLYESSWAHTYVQAGVPVPASGTHDHTLRAVGQGLTPEELWARLRVRW
                     TFGRPIDGAAS"
     gene            complement(2597..3508)
                     /gene="xerD"
                     /locus_tag="PK242_06120"
     CDS             complement(2597..3508)
                     /gene="xerD"
                     /locus_tag="PK242_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOZ93628.1"
                     /translation="MAAAAPDDPLIDRFIDALWLEDGLSANSLAAYRRDLTLYAAWLQ
                     ATHRVALAQTQEPHLNAYMAARADGKATSANRRLTVFKRFFRWALREHLVSADPTLRL
                     LAARQPLRVPKTLGEAQVDALLTAPNTGTPLGLRDRAMLELMYASGLRVSELVALRSF
                     HVGFNEGVLRVLGKGSKERLVPFGEQARDWLERYLREARPALLGGRQSDALFVTSAGR
                     TPGTAMSRVMFWNLVKRYARTAGVTAPLSPHTLRHAFATHLLNHGADLRAVQLLLGHA
                     DISTTTIYTHIARQRLKELHAQHHPRG"
     gene            complement(3688..4653)
                     /locus_tag="PK242_06125"
     CDS             complement(3688..4653)
                     /locus_tag="PK242_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugE"
                     /protein_id="HOZ93629.1"
                     /translation="MQRRQWIVLAAATLSAGAVCAQAYPSKPIRLVVPFAPGGTTDII
                     ARTIADPLGKALGQAVVVENKAGGGGVIGATEVVRAAPDGYTLSVATVSTTAANPAIN
                     KKIPYDPINDFVPVINIAATPNVIAVHPSFPAKDYASFVALLKKAPGKYSYASSGTGG
                     IGHLQTELWKSLAGVFVTHIPYRGAGPALNDTVAGQVPIIFDNLPSALPFIKGGKLVP
                     LVVAAPERLADLPNVPTFKEIGLPAVNRLAYYGILAPKGTSKEIVDKLNAATRKVLED
                     PAVKKRIADTGSIMVANTPEQFAQQIKDEFAVYKEVVAKQKLTLD"
     gene            4761..5678
                     /locus_tag="PK242_06130"
     CDS             4761..5678
                     /locus_tag="PK242_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOZ93630.1"
                     /translation="MHHVTLLLPDFSLIALGWLLCRHTALGRGVWQAVEQLVYFFLFP
                     VYLFQSIVRAPLDVQAASSLIGAGLALGAAGIALAWALPHLPLLGRHIDAHDHAASAQ
                     IAFRFNSFIGLAVAERLAGAQGQQLMAVLIGVSVPLYNVAAVWPMARRSGRAIGGELL
                     RNPLILATVLGLVANLLGLGLPDWAATTLSRIGSASIALGLMAAGAGMQLQSLARAKL
                     LGTALLTIRHLLQPLIAWLLARAFGLGAAEAAALLVFAALPTSSSGYVLAARMGYNGP
                     YVAGLVTLSTLLALLSLPWAFTLLERVAP"
     gene            complement(5853..6902)
                     /gene="queG"
                     /locus_tag="PK242_06135"
     CDS             complement(5853..6902)
                     /gene="queG"
                     /locus_tag="PK242_06135"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOZ93631.1"
                     /translation="MRGWARELGFSHLGVADIDLSAAEPGLLAWLHNGFHGRMDYMAA
                     HGLRRARPAELVPGTVSVITARMDYLPRATPEDWQDVELARLGRPQEGVVSLYARGRD
                     YHKVLRGRLQKLQDRIALAIGPFGHRVFTDSAPVLEVELASRSGQGWRGKHTLLLSRE
                     GGSMFFLGEIYVDLHLPRTAPTGAHCGQCSACIDACPTGAIVAPYRLDARRCISYLTI
                     EHDGAIDEALRPLIANRIYGCDDCQLVCPWNKYAGRSALPDFDPREGLGGAQLTDLLD
                     WSEDEFLRRTEGSPIRRIGHERWLRNVAVALGNALAALPPGDARRAPLHAALRRRTNH
                     PSALVREHVRWALGR"
     gene            6939..7445
                     /gene="tsaE"
                     /locus_tag="PK242_06140"
     CDS             6939..7445
                     /gene="tsaE"
                     /locus_tag="PK242_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOZ93632.1"
                     /translation="MSSHAQIVGSPDAPALSLLWRSEDDTAAFARRLAAAPELRQACI
                     ELHGELGAGKTSFVRHLLRALGVAGRIKSPTYAVVEPHQTPDGLAVSHFDFYRFTDPR
                     EWEDAGLRDLFAAPGLKLSEWPDKAAGLLPAPDLALRLTVRDDGARAVRVSAHTPRGR
                     RLLTELGA"
     gene            7442..8836
                     /locus_tag="PK242_06145"
     CDS             7442..8836
                     /locus_tag="PK242_06145"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOZ93633.1"
                     /translation="MTPVTPESPGWPPAPGRRRALLQGGSLVLWLGAHQLARGAGILA
                     VRVWPAEDYSRVTVESDTALKASYRLVGTPPRLAVDIDGLTLDPTLRELVAKVRADDP
                     NIAGIRVGQHQPGVVRLVFDLKHEIKPQVFTLGPVAAYQHRLVFDLYPAHPPDPLAQL
                     IAERMRELGRSPAPVPVEPAAAPAHGGTDTLGELIARQIERANELTRRDAARRPAPPP
                     AGAGATERLIIVALDPGHGGEDPGAIGPGGTQEKDVVLQVALKLRERINASSVGGNPM
                     RAFLTREGDYFVPLQTRVEKARRVQADLFVSIHADAFMRPEASGASVYALSERGASST
                     AARWLAQQENASDRVGGVSVSVKDQSVRHALLDMSTTAQIKDSLRLGDSMLREIGGVG
                     KLHKRSVERANFAVLRNPDVPSVLVETAFISNPDEERRLRSQAYQNELADALISGIRK
                     YFTANPPLARHRQV"
     gene            complement(9017..9664)
                     /locus_tag="PK242_06150"
     CDS             complement(9017..9664)
                     /locus_tag="PK242_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOZ93634.1"
                     /translation="MDVLMMLVDFVLHVDRHLGEFVAAYGAWVYALLFLIVFVETGLV
                     VMPFLPGDSLLFVVGALAGAGHLSWPLACAVLLAAAILGDQCNYSLGRYFGPRVFKWE
                     SSRLFNRQAFDRAHRFYERYGGVTVILARFMPFIRTFVPFVAGVAEMTRHRFTLFNVA
                     GALIWVLGVVSAGYLFGNLPWVQANLSKIIWALILVPGLIALYGGWKAGRAAPSA"
     gene            9846..10970
                     /locus_tag="PK242_06155"
     CDS             9846..10970
                     /locus_tag="PK242_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOZ93635.1"
                     /translation="MSKPTQTTGRRNLLKGAAVAAGAMSVPMISVAQTTTLRFQSTWP
                     AKDIFHEFAQDYAKKINDMAGGRLKIEVLPAGAVVPAFQLLDAVAKGTLDGGHGVVAY
                     WYGKNQAVALWGSGPAFGMDANMVLAWHNYGGGKELLDEIYKSLNLDVVSKLYGPMPT
                     QPLGWFKKPVAKVEDLKGLKFRTVGLAVDVFTEMGVAVNPLPGGEIVPALDRGLLDAA
                     EFNNASSDRVLGFPDVAKNCMLQSFHQSSEQFEVLFNKTKYDALPAELKAIIDYATQA
                     ASADMSWKAADRYAKDYAEMASKQGVKFYKTPDAILRAQLAAWDKVTAKKAAENPMFK
                     KVMESMRAFAQRSGRWQNDTTIDYRMAWNHYFGAKKGAKG"
     gene            11088..11747
                     /locus_tag="PK242_06160"
     CDS             11088..11747
                     /locus_tag="PK242_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOZ93636.1"
                     /translation="MQKLLLGIDAVSTFIGKAAAWACVLLTALICWEVFSRYALNKPH
                     AWVLDAQIMLYGTMFMMAGAYTLAKNGHVRGDVLYGFFSPRVQAGLDLLLYFLFFLPG
                     VLAMTWAGWGYAQESLAIREQTFSAEPLPLYPFKFVIPLAGAALLLQGLAEIARCVIC
                     LRQGAWPSREADVEEVDVEKLKEMVHVTDADRAAIAVQPPRPSPPSPGHADHGHTDRG
                     A"
     gene            11744..>13167
                     /locus_tag="PK242_06165"
     CDS             11744..>13167
                     /locus_tag="PK242_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOZ93637.1"
                     /translation="MSMRKELWFGFSIMALVVASAAWLLLGVEHITNGHLGLLMLSLV
                     VVAIMLGFPTAFTLMGMGMIFTWLAYDRNVTRTLDLMVQAAYKSMANDVLIAIPLFVF
                     MGYLVERANLIEKLFKSLHLAMARVPGSLAVATLVTCAIFATATGIVGAVVTLMGLLA
                     LPAMLRAGYSIPMSAGAITAGGCLGILIPPSVLLILYGATAGVSVVQLYAGAFFPGVM
                     LAGLYVAYVIIVAKLKPAWAPPLSAKERQVDLPALTSALPAGTRDYAPLRLLNALKGR
                     RNADVPLAHVLRQLAVVVLPGLLFLLLATSTWQAVTKVPAEQAVELQAIGSEGSGAES
                     SDSGLAEPPAEDELQEPPTEDALQEPPGADAPDTLATPPGAEVDAASEPTAAGVAEPP
                     GASAAASDAAGAAQAGTQPAPTWWWVVMGVLGACTALFYLFLSFARLEIFKMLLGSFF
                     PLMILILAVLGSIVFGLATPTEAAA"
BASE COUNT         2048 a         4633 c         4497 g         1989 t
ORIGIN      
        1 gccgaccgct tcaccaccgg ctcgtcgatc atgccgcaga agaaaaaccc cgacgtgccc
       61 gaactggcgc gcggcaagac cggccgcgtg gtcggccacc tgatgggcct gatcaccctg
      121 atgaagggcc agccgctggc ctacaacaag gacaaccagg aagacaagga gccgctgttc
      181 gacaccgtgg acacgctgcg cgacaccctg cgcatcttcg tcgagatgat cggcggccag
      241 cccaacccgg tcagcggcgc caaggaaggc ggcatcagcg tgaaccccgc cgccatggag
      301 gcggcggcgc agaagggcta cgccaccgcc accgacctgg ccgactactt ggtcaagaag
      361 ggcctgccct tccgcgacgc acacgaaacc gtggcccacg ccgtgaaagc cgcccagtcg
      421 cacgcctgcg acctgtcgga gctgccgctg aaggtgctgc aggaattcca cccggccatc
      481 gacaaggacg tctaccaggt cctgagcctg cgcggcagcc tgaacgcccg cgccaccctg
      541 ggtggcacgg cgccggcgca ggtgcagaca cagatcgcac gacaccgggc gcggctggcg
      601 cttggctgag cggcgtcgct gactgggcga acggcccacc ctcccggcgc gcgccgcaat
      661 ctgcgtcggc gcgggcaagc ccctccaacc gccgcttcca ggctgagtgg ggggatcagg
      721 tgttggccgc cagatccacc accggctggc tcccgcacgg caaccgatcc gcaccgacgg
      781 caccttgtgc gcggaaccac tcgatggtct gcgccaggcc ttgcaccaag ggcacttccg
      841 gttgccattc gagccaacgc tgtgcctggc tgatgtcggg ctggcgacag cgcgggtcct
      901 cttcacgtgc cggcagcgtc cgcacgccag cgttgctgcc ggtcaggcgc agcaccatgg
      961 cagccaacgc atgcatcgac acctcctgcg gattgcccag gttgatcgga cccgtcacgc
     1021 gctcctgcgc catcagggcc agcagaccac gcacggtatc gctgacgtag cagaaggagc
     1081 gggtctgccg accatcgccg tgcagcggca gcggctctgc gcgcaacgcg ctggtgatga
     1141 acgtgctgac cacgcgaccg tcctgtggtc gcaggcgcgg gccataggtg ttgaacagac
     1201 gcgccagccg cacctgggtg ccgtgctgcc gggcatgcgc caccgtcagg gcctccgcgc
     1261 aacgcttgcc ctcgtcgtag caactgcgcg ggcccaccgg gttgacgtgg ccccggtagg
     1321 cctcggtctg tggatgcacc tgcgggtcgc cgtacacctc gctggtggaa gtcagcaaca
     1381 tgcgggcacc gtgggcccgc gcaaagtcca gcgcgttcag ggtgcccagg acgttggtgc
     1441 gaatggtctg caccggcgcg gcctggtagt cgaccgggct ggccgggctg gccaggtgaa
     1501 acacgcgctg ggcagccggc aatcgctgcc acaaggccgg atccaccacg tccccaggca
     1561 cgaaggcaaa cccagggacc gccagcaggt gcgccacgtt gtcgctgctt ccactggaca
     1621 ggtcgtccac gcacgtgact tgaacgccct ggatcagcag ggcctcgcaa aggtggctgc
     1681 cgatgaagcc ggccccgccg gtaaccacgg cacgggtcat gacgcggcgc catcgatggg
     1741 gcgaccgaat gtccagcgca cccgcaaccg tgcccacagc tcttcgggcg tcaatccttg
     1801 acccacggcg cgcaaggtgt ggtcgtgcgt cccgctcgcc ggcacgggca cgccggcctg
     1861 tacgtaggtg tgcgcccagc tggattcgta cagcaccgtg cgttggccag tccaggccac
     1921 cttgaggcga tgatgctgca tgagccagca catgacccgg tcacagatcg gcgtcaacga
     1981 gggcggtatc tggcaccacg caccccacag aaagcccgcc gagcgcgtca gactgatgca
     2041 actggtgtcg acgaatccag agcccacgtc ttcgcgcggc gccaccaacc gcagatgttc
     2101 gtgacccggc aagaacaggt accgcagcga aaacaccgcg tccagagcaa ccccggcagc
     2161 ctgcgccttg gcaacggttt ccacgacggt ggcgacatgc tcgggctcga tcagattgtc
     2221 ggcgtccaga aagcacacca catcgaaacc atcggccagc gcgcactggg cgcccacacc
     2281 tcgcggggta cagccgaaat tggcgtggcc gttcggcagc tggatgtggc gcacgcctgg
     2341 ccactgcgtc agcaaggcgt tctgaggaaa gccgtcggcc accatgtagt gaaccacctg
     2401 cgggtaggtc tgggcgcgaa cgctggtcaa gcagcgctgc agcgtcgccg gtgactcctt
     2461 gaaatacggc gtcaccacag ccacacgcag tccagcggcg catccgctcg attcgctgac
     2521 tgtcacgatc ctttcccctt tcgtcagcag gggattatgc aggttgatgc atcgcaccat
     2581 gctcaggcac ttgccctcac ccccgcggat ggtgctgcgc gtgcaattcc ttcagccgct
     2641 ggcgtgcgat gtgggtgtag atggtggtgg tggaaatgtc ggcatgcccc agcagcagct
     2701 gcaccgcacg caggtcggct ccgtggttga gcaggtgggt ggcgaaggcg tggcgcagcg
     2761 tgtgcggtga cagcggcgcc gtcacgccgg cggtgcgcgc atagcgcttg accaggttcc
     2821 agaacatcac gcgcgacatc gcggtgccgg gcgtgcggcc ggcgctggtg acgaacagcg
     2881 catcgctctg gcgcccgccc agcagcgccg gacgggcctc gcgcaggtag cgctccagcc
     2941 agtcgcgcgc ctgctcgcca aacggcacca ggcgctcctt gctgcccttg cccagcacgc
     3001 gcagcacgcc ttcgttgaaa ccgacgtgaa agctcctgag cgccaccagt tcgctcacgc
     3061 gcaggccgct ggcgtacatc agttccagca tggcgcggtc gcgcaggccg agcggggtgc
     3121 cggtgttggg cgcggtcagc agggcatcga cctgcgcctc gccgagcgtc ttgggcacgc
     3181 gcagcggctg gcgcgcggcc agcagcctga gcgtcgggtc ggcgctcacc aggtgttcgc
     3241 gcagcgccca gcgaaaaaag cgtttgaaca ccgtcagccg ccggttggcc gaggtggcct
     3301 tgccgtcggc gcgcgcggcc atgtaggcgt tcaggtgcgg ctcctgggtc tgcgccagcg
     3361 ccacgcggtg cgtggcctgc agccaggcgg cgtacagggt gaggtcgcgc cggtaagcgg
     3421 ccagggagtt ggccgacaga ccgtcttcca gccacagggc gtcgatgaag cggtcgatca
     3481 gcgggtcgtc gggtgcggca gcggccatgg gtcggcgcga tgcaatgggc atcgatgcga
     3541 gcgccggatt cggtgtcata ttgggctctg gcacgcgccc atcaagcgcc agcagctacg
     3601 ttaatgatag caaccaaagt ccgctgcgat ccgggcacgc cgccggcctg ggacctgcgg
     3661 gcgtgtccgg tcgcgggccg gcgccgatca atcgagcgtc agcttctgct tggccaccac
     3721 ctccttgtag acggcgaact cgtccttgat ctgctgggcg aactgctcgg gcgtgttggc
     3781 caccatgatg gagccggtgt cggcgatgcg cttcttgact gccggatctt ccagcacctt
     3841 gcgggtggcg gcgttcagct tgtcgacgat ctccttggac gtgcccttgg gcgccaggat
     3901 gccgtagtag gccagacggt tgaccgccgg cagaccgatt tccttgaagg tcggcacgtt
     3961 gggcagatcg gccaggcgtt cgggcgcggc gaccaccagc ggcaccagct tgccgccctt
     4021 gatgaagggc agcgccgaag gcaggttgtc gaagatgatg ggcacctggc cggccacggt
     4081 gtcgttgagc gccgggccgg cgccgcggta cggaatgtgg gtgacgaaca caccggccag
     4141 gctcttccac agctcggtct gcaggtggcc gatgccgccg gtgccggagg acgcgtagga
     4201 gtacttgcct ggcgccttct tgagcagtgc aacgaaggag gcgtagtcct tggccggaaa
     4261 gctggggtgc acggcgatca cgttgggcgt ggcggcgatg ttgatcaccg gcacgaagtc
     4321 gttgatcggg tcgtacggaa tctttttgtt gatggccggg ttggcggccg tggtggacac
     4381 cgtggccacg ctgagggtgt agccgtcggg ggcggcgcgc accacttcgg tggcgccgat
     4441 cacgccgccg ccgccagcct tgttttcgac caccacggcc tgccccagcg ccttgcccag
     4501 cgggtcggcg atggtgcggg cgatgatgtc ggtggtgccg ccgggtgcaa acggcaccac
     4561 caggcgaatc ggcttgctgg ggtaggcctg cgcgcacacc gcgccggcgg acagggtggc
     4621 agcggcgaga acgatccatt gacgtcgttg catggcggtc cggttcctct gagtggttga
     4681 aggataaaaa agcgcgccat gttaaccatg ccgccgccgg cctggccatt agggattgcg
     4741 caggccgcta tgctcgcgcc gtgcaccacg tcacgcttct gctgcccgat ttttcgctga
     4801 ttgcgctcgg ctggctgctg tgtcgccaca cggccctggg ccgtggcgtc tggcaggcgg
     4861 tggaacaact ggtgtacttc tttctgtttc cggtctacct gtttcagtcc atcgtgcgcg
     4921 cgccgctgga cgtgcaggcc gcatccagcc tgatcggcgc cggcctggcc ctgggtgccg
     4981 ccggcatcgc cctggcctgg gccttgccgc atctgccgct gctggggcgg cacatcgacg
     5041 cccacgacca cgcggccagc gcccagatcg cgtttcgctt caattccttc atcggcctgg
     5101 cggtggccga gcgcctggcc ggcgcccagg gccagcagtt gatggcggtg ctgatcgggg
     5161 tcagcgtgcc gctctacaac gtggccgcgg tgtggccgat ggcgcgccgc agcggccgcg
     5221 ccatcggcgg cgagctgctg cgcaacccgc tgatcctggc caccgtgctg gggctggtgg
     5281 ccaacctgct gggcctcggc ctgccggact gggccgccac cacgctgtcg cgcatcggct
     5341 ccgcgtcgat cgcgctcggg ctgatggcgg ccggcgccgg catgcagctg cagagcctgg
     5401 cgcgcgccaa gctgctgggc accgcgctgc tgaccatccg ccacctgctg caaccgctga
     5461 tcgcctggct gctggcgcgc gcctttggcc tgggcgcggc cgaagcggcg gcgctgctgg
     5521 tgtttgccgc cctgcccacc tcgtccagcg gctacgtgct ggccgcgcgc atgggctaca
     5581 acggccctta cgtggcgggg ctggtcacgc tgtcgaccct gctggcgctg ctgagcctgc
     5641 cctgggcgtt cacgctgctg gagcgggttg ccccttgaag cgaggctgcc ggcgccttgc
     5701 tggcgcgcgc ggcgacccct cttgttcgag gtctgcgcct ggccacgccg atcgcgggtt
     5761 gacgcgcccg acgaaatgct ataaatacag cagcatgcgg cgccgatccc gcgccatgcg
     5821 agccggcaaa aaccatagtg atcgccccga tgtcaacgcc ccagcgccca ccgcacatgc
     5881 tcgcgcacca gcgcgctcgg gtggttggtg cggcgcctca gggcggcgtg caggggcgcg
     5941 cgccgcgcgt cgccgggcgg cagcgcggcc agggcgttgc ccagcgccac ggcgacgttg
     6001 cgcaaccagc gctcgtggcc gatgcggcgg atcgggctgc cttcggtgcg gcgcaagaac
     6061 tcgtcttcgc tccagtccag cagatcggtc aactgggcgc cgcccaggcc ctcacgcggg
     6121 tcgaaatcgg gcagcgcact gcgcccggcg tatttgttcc acggacacac cagctggcag
     6181 tcgtcgcagc catagatgcg gttggcgatc agcgggcgca gcgcctcgtc gatggcgccg
     6241 tcgtgctcga tcgtcaggta ggagatgcag cggcgcgcgt ccagccggta cggcgccacg
     6301 atggcgcccg tcgggcaggc gtcgatgcag gcgctgcact ggccgcagtg ggcgccggtc
     6361 ggtgcggtgc gcggcaggtg caggtcgacg tagatttcac ccagaaagaa catcgagccg
     6421 ccttcgcgcg acagcagcag cgtgtgcttg ccgcgccagc cctggccgct gcggctggcc
     6481 agctccacct ccagcaccgg cgccgaatcg gtgaacacgc ggtggccgaa cgggccgatg
     6541 gccagcgcga tgcggtcctg cagcttctgc aggcgcccgc gcagcacctt gtgatagtcg
     6601 cgcccgcggg cgtagagcga caccacgccc tcctgcgggc ggcccagccg cgccagctcc
     6661 acgtcctgcc agtcctcggg ggtggcgcgc ggcagataat ccatgcgcgc ggtgatcacg
     6721 ctcaccgtgc cgggcaccag ttcggccggg cgcgcgcgcc gcaggccgtg cgccgccatg
     6781 tagtccatgc ggccgtgaaa accgttgtgc agccaggcca gcaggccggg ctcggcagcc
     6841 gagaggtcga tgtcggccac gcccaggtgc gaaaacccca gctcgcgcgc ccagccgcgc
     6901 aactggttca acagatcaag ggaatcgatt tgagcgccat ctccagccac gcccaaattg
     6961 taggaagccc cgatgcgccc gccctcagcc tgctgtggcg cagcgaggac gacaccgccg
     7021 cctttgcccg ccgcctggcg gccgcgcccg agctgcgcca ggcctgcatc gagctgcacg
     7081 gcgagctcgg cgccggcaag accagcttcg tgcgccacct gctgcgcgcc ctgggcgttg
     7141 ccgggcgcat caagagcccg acctatgccg tggtcgaacc ccaccagacg cccgacgggc
     7201 tggcggtgtc gcatttcgac ttctaccgct tcaccgaccc gcgcgagtgg gaagacgccg
     7261 gtctgcgcga cctgttcgcc gcgcccggcc tgaagctgtc cgaatggccc gacaaggccg
     7321 ccggcctgct gccggcgccc gacctggcgc tgcggctgac ggtgcgcgac gatggcgccc
     7381 gcgccgtgcg cgtcagcgcc cacacgccgc ggggtcggcg cctgctgacg gagctgggcg
     7441 catgacgccg gtcacccccg agtcgcccgg ctggccaccg gcacccgggc ggcgccgcgc
     7501 actgctgcag ggcggctcgc tggtgctgtg gctgggcgcg caccagttgg cgcgcggagc
     7561 cggcatcctg gcggtgcgcg tgtggccggc cgaagactat tcgcgcgtca ccgtcgaatc
     7621 cgacaccgcg ctcaaggcca gctaccgcct ggtcgggacg ccgccgcggc tggcggtgga
     7681 catcgacggc ctgacgctcg accccacgct gcgcgagctg gtggccaagg tgcgcgccga
     7741 cgaccccaac atcgccggca tccgcgtggg ccagcaccag cccggcgtgg tgcgcctggt
     7801 gttcgacctc aagcacgaga tcaagccgca ggtgttcacc ctggggccgg tggcggccta
     7861 ccagcaccgg ctggtgttcg atctgtaccc ggcgcacccg cccgacccgc tggcccagct
     7921 catcgccgag cgcatgcgcg agctcggccg cagccccgcg ccggtgccgg tcgagccggc
     7981 ggccgcgccc gcgcacggcg gcaccgacac gctgggtgag ctgatcgcgc gccagatcga
     8041 gcgcgccaac gaactcaccc gacgcgacgc cgcgcgccgc cctgccccac cgccggccgg
     8101 cgccggcgcc accgagcgcc tgatcatcgt ggcgctcgat ccgggccacg gcggcgagga
     8161 cccgggcgcc atcggccctg gcggcacgca ggaaaaggac gtcgtgctgc aggtggcgct
     8221 gaagctgcgc gagcgcatca acgccagcag cgtgggcggc aacccgatgc gcgccttcct
     8281 gacgcgcgag ggcgactact tcgtgccgct gcagacgcgg gtcgaaaagg cacggcgggt
     8341 gcaggccgac ctgttcgtca gcatccacgc cgacgccttc atgcggcccg aggccagcgg
     8401 cgccagcgtc tacgcgctgt ccgagcgcgg cgcctccagc accgccgcgc gctggctggc
     8461 gcagcaggaa aacgcctccg accgcgtcgg cggcgtcagt gtcagcgtga aagaccagtc
     8521 ggtgcggcac gcgctgctgg acatgagcac caccgcccag atcaaggaca gcctgcggct
     8581 gggcgattcg atgctgcgcg agatcggcgg cgtcggcaag ctgcacaagc gcagcgtcga
     8641 gcgcgccaac ttcgccgtgc tgcgcaaccc cgacgtgccc agcgtgctgg tcgagaccgc
     8701 cttcatcagc aaccccgacg aagagcgccg cctgcgcagc caggcctacc agaacgagct
     8761 ggccgacgcc ctgatcagcg gcatccgcaa gtacttcacc gccaacccgc cgctggcgcg
     8821 ccaccggcag gtctgaggtg gtgcagatgg cgggtgcgtg cgctgcgctt cgatgaccca
     8881 gggggtgagg cgtcacgcca cgggcaggca gcgtccccgg cccgcctgtg accctggaaa
     8941 cggcgtcaag aatactattg ttttgatagc ttccggcgca atccggtagc gcgccagagc
     9001 cggttttgat acctgatcag gcgctgggcg cggcgcggcc cgccttccag ccgccgtaca
     9061 gcgcgatcag gcccggcacc aggatcaggg cccagatgat cttgctcagg ttggcctgca
     9121 cccagggcag gttgccgaac aggtagcccg cgctcaccac ccccagcacc cagatcagcg
     9181 ccccggcgac gttgaacagc gtgaagcggt ggcgcgtcat ctcggccacg ccggccacga
     9241 agggcacgaa ggtgcggatg aacggcatga agcgcgccag gatcaccgtg acgccgccgt
     9301 agcgctcgta gaaccggtgc gcgcggtcga acgcctgccg gttgaacagg cgtgagcttt
     9361 cccacttgaa cacgcgcggc ccgaaatagc gccccaggct gtagttgcac tggtcgccca
     9421 ggatcgccgc cgccagcagc accgcgcagg ccagcggcca gctcaggtgc ccggccccgg
     9481 ccagggcgcc gaccacgaac agcagcgaat cgccgggcag aaacggcatc accaccaggc
     9541 cggtctcgac gaacacgatc agaaacagca gcgcgtacac ccaggccccg taggccgcca
     9601 cgaactcgcc caggtggcga tcgacgtgca gcacgaagtc caccagcatc atcaggacat
     9661 ccatggcccc gattatccgg cgcccagcga cgcgccggcg gcacgccgcg ccccccggca
     9721 ggcgtggttt tcgcgcacca cggccgcgcc cctcggtgaa aaccctcttt gaacacaagg
     9781 gcttgcgatc ggatcatcac ccctctggac tgtccagaca ccaacccacc cggagacaga
     9841 ggaagatgag caaacccacc cagaccaccg gtcgtcgcaa cctgctgaag ggcgccgccg
     9901 tcgccgccgg cgccatgtcg gtgcccatga tcagcgtggc gcagaccacc accctgcgtt
     9961 ttcagagcac ctggccggcc aaggacatct tccacgagtt cgcgcaggac tacgccaaga
    10021 agatcaacga catggccggt ggccgcctga agatcgaggt gctgcccgcc ggcgccgtgg
    10081 tgccggcctt tcagctgctc gatgccgtgg ccaagggcac gctcgacggc ggccatggcg
    10141 tggtggccta ctggtatggc aagaaccagg ccgtggcgct ctggggctcg ggcccggcct
    10201 tcggcatgga cgccaacatg gtgctggcct ggcacaacta cggcggcggc aaggagctgc
    10261 tcgacgagat ctacaagagc ctgaacctgg acgtggtctc caagctctac ggccccatgc
    10321 ccacgcagcc gctgggctgg ttcaagaagc ccgtggccaa ggtggaagac ctgaagggcc
    10381 tgaagttccg caccgtgggc ctggccgtgg acgtgttcac ggagatgggc gtggccgtca
    10441 atcccctgcc cggcggcgag atcgttccgg cgctcgaccg cggcctgctc gacgcggccg
    10501 agttcaacaa tgcgtcgagc gaccgcgtgc tgggcttccc cgacgtggcc aagaactgca
    10561 tgctgcagag ctttcaccag tccagcgaac agttcgaggt gctgttcaac aagaccaagt
    10621 acgacgcgct gccggccgag ctcaaggcca tcatcgacta cgccacgcag gcggccagcg
    10681 ccgacatgag ctggaaggcc gccgaccgct acgccaagga ctacgccgag atggcctcca
    10741 agcagggcgt gaagttctac aagacgcccg acgccatcct gcgcgcgcag ctcgccgcct
    10801 gggacaaggt gacggccaag aaggcggccg agaaccccat gttcaagaag gtcatggagt
    10861 ccatgcgcgc gtttgcccag cgcagcgggc gctggcagaa cgacaccacc atcgactacc
    10921 gcatggcctg gaaccattac ttcggcgcca agaagggcgc caagggctga cgccgcccgc
    10981 attcgagaag gcctgcgcaa ggccttcttt tttgtcgacc cggggccctg ggcgaaatcg
    11041 atcgccgggc cctgttcgtt ttcatcggcc ctcgcggctg tttcgccatg caaaagttgc
    11101 ttctcgggat cgacgccgtg tcgaccttca tcggcaaggc cgccgcctgg gcctgcgttc
    11161 tgctcacggc cctgatctgc tgggaagtgt tttcgcgcta cgccctcaac aagccccacg
    11221 cctgggtgct ggacgcgcag atcatgctgt acggcaccat gttcatgatg gccggcgcct
    11281 acaccctggc caagaacggc catgtgcgcg gtgacgtgct gtacggcttc ttcagcccgc
    11341 gcgtgcaggc cgggctggac ctgctgctgt actttctgtt cttcctgccc ggcgtgctgg
    11401 ccatgacctg ggccggctgg ggttatgcgc aggaatcgct ggcgatccgc gagcagacct
    11461 tctcggccga gccgctgccg ctgtacccct tcaagttcgt cattccgctg gccggcgcgg
    11521 cgctgctgct gcagggtctg gccgagatcg cccgctgcgt gatctgcctg cgccagggtg
    11581 cctggcccag ccgcgaggcc gacgtcgagg aagtggacgt ggaaaaactc aaggaaatgg
    11641 tgcacgtcac cgacgccgac cgcgccgcca tcgcggtgca gccgccccga ccgtcgccgc
    11701 cgagccccgg gcacgcggac cacgggcaca ccgaccgggg ggcctgagca tgcgcaagga
    11761 actctggttc ggcttttcca tcatggcgct ggtggtggcc agcgccgcct ggctgctgct
    11821 gggcgtggag cacatcacca acggccacct gggcctgctg atgctgtcgc tggtggtggt
    11881 ggccatcatg ctgggctttc ccaccgcgtt cacgctcatg ggcatgggca tgatcttcac
    11941 ctggctggcg tacgaccgca acgtcacgcg cacgctcgac ctgatggtgc aggcggccta
    12001 caagagcatg gccaacgacg tgctcatcgc catcccgctg ttcgtcttca tgggctacct
    12061 ggtcgagcgc gccaacctga tcgaaaagct gttcaagagc ctgcacctgg ccatggcgcg
    12121 cgtgccgggc tcgctggcgg tggccacgct ggtgacctgc gccatcttcg ccacggccac
    12181 cggcatcgtc ggcgccgtgg tcacgctgat ggggctgctg gcgctgccgg ccatgctgcg
    12241 cgcgggctac agcatcccca tgtcggcggg cgccatcacc gccggcggct gcctgggcat
    12301 tctgattccg ccctcggtgc tgctcatcct gtacggcgcc acggccgggg tgtcggtggt
    12361 gcagctgtat gccggcgcct tcttccccgg cgtcatgctg gccgggctgt atgtggccta
    12421 cgtcatcatc gtggccaagc tcaagcctgc ctgggcgccg ccgctgtcgg ccaaagagcg
    12481 ccaggtcgat ctgccggccc tgaccagcgc cctgccggct ggcacgcgcg actacgcgcc
    12541 gctgcgcctg ctgaacgcgc tcaagggccg gcgcaacgcc gacgtgccgc tggcccacgt
    12601 gctgcgccag ctggcggtgg tggtgctgcc gggtctgctg tttctgctgc tggccaccag
    12661 cacctggcag gccgtgacca aggtgccggc cgagcaggcg gtggagctgc aggccatcgg
    12721 atcggagggc agcggcgccg aaagctccga ctccgggctg gccgagccgc cggccgaaga
    12781 tgagttgcag gaacctccga ccgaggacgc cctgcaggag ccgccagggg ccgatgcgcc
    12841 cgacaccctg gccaccccgc ccggcgccga ggtcgatgcc gcgtccgagc cgacggccgc
    12901 cggcgtggcc gagccccccg gggccagcgc cgcggcctcc gatgccgccg gcgcagccca
    12961 ggccggcacg cagccggcgc ccacctggtg gtgggtcgtg atgggggtgc tgggcgcctg
    13021 caccgccctg ttctacctgt tcctcagctt cgcccggctg gaaatcttca agatgctgct
    13081 gggctcgttc ttcccgctga tgatcctgat tctggcggtg ctgggctcca tcgtgttcgg
    13141 cctggccacg ccgaccgagg cggcggc
//
LOCUS       DAOOOG010000059        13073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_5251_length_13073_cov_27.254724, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000059 DAOOOG010000000
VERSION     DAOOOG010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13073
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5251_length_13073_cov_27.254724"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..779)
                     /locus_tag="PK242_06170"
     CDS             complement(108..779)
                     /locus_tag="PK242_06170"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOZ93638.1"
                     /translation="MWCIGELTEEYRHRTYALLALYAQPFDPREPVVCIDEKSLQLIG
                     HSRAPLPMSPGILAKDDYEYVRRGTSNLFVAVEPKAGQRLIAVTAQRGKVDFVEFIAS
                     LLGGAYARARRVHLVLDNLNIHFRKCFDDVLGCRAAAKLLRRVQFHYTPKHASWLNMA
                     EIEIGILSRQCLDRRWADRHTLRREVEQWQLARNAAKQTIEWKFTRQDADRKLGRHYV
                     PLFTC"
     gene            complement(779..1222)
                     /locus_tag="PK242_06175"
     CDS             complement(779..1222)
                     /locus_tag="PK242_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ93639.1"
                     /translation="MRQTQLHLSEEDRAVVEEVRSKGLHRSREIHRAHMLACLDRGVP
                     EAQILAVLGVGRTALWRTRAAYLQGGLELALFDVTRPGRPRTYDTDVEAQVTALACSA
                     PPEGRKRWTMVELERAARREPALGNLSRETVRRMLKKTTSNPGGG"
     gene            complement(1311..2024)
                     /locus_tag="PK242_06180"
     CDS             complement(1311..2024)
                     /locus_tag="PK242_06180"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ93640.1"
                     /translation="MSRVARSPRRSADANPVASAPARRRRLSKEERWEEILVVAEQVF
                     SEMGYDSATVAEVARRAGVVEGNVYRYVKTKRELLAHVISEWYSRTNAELENELRHIT
                     SVRSRLRFLIAAHLRALRDSPGLMRLIIRELRTSDTEFRAIVGKLNQRYTWHLRHAIE
                     CGVESGEFGRDIPVRLVRDMIFGGIEHHATRYLMGEGSLDVESTADQILFLLLHGVQA
                     PPGAGNARRSRKPGDKPLT"
     gene            complement(2078..2854)
                     /locus_tag="PK242_06185"
     CDS             complement(2078..2854)
                     /locus_tag="PK242_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ93641.1"
                     /translation="MRMRDAVVLISGAASGLGWGAAQYCVREAGARVVMLDRNAEQGA
                     ARAAELDPGRCLFVQADLADEAQVAGAVQRAVEHFGSLHACFSFAGVVSPMRMLDRDG
                     KASSGATFARTVAINLVGTFHLLSHCAQQMQRNPPDDDGERGVLVTIASGAAFDGQVG
                     QTAYASSKAGLVGLCLPAARELAGIGIRINTIAPGAFWTPMLESLPETVLERIQADFQ
                     FPRRFGRAEEIAALCCHIAENHFINGECIRLDGAFRLPPR"
     gene            complement(2851..4095)
                     /locus_tag="PK242_06190"
     CDS             complement(2851..4095)
                     /locus_tag="PK242_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOZ93642.1"
                     /translation="MTQVLIVGVGMTPFARHPELTAKQLVEWAVRDALADAGCEPAQV
                     QSAFYGNCAQGLLDGQHLVRGQMALLPLALQGIPIINVENACATASTALHMAFHYLRS
                     GACDIALAVGVDKLFTAERERRFAIFDAAWDVDTPAQNHAHFTALGKGVAIPEGSVSP
                     AQYSPFMDVYAGFARQYMERFGITQRQLAAIAAKNRNHAVANERAQFRQAMTVEEVLA
                     SPPITYPLTLPMCAPISDGAAAALLCTPAGLARLQGLRRRAVIVHASVLRTATARDAQ
                     DLEHHLTRLAAQQAYEAAGMGPGDMHLAEVHDATAMGELMQTENLGFCGLGEGGELAE
                     RGETTLGGRIPVNPSGGLESKGHPIGATGLGQIFELVAQLRGECGSRQVQGARLAVQE
                     NAGGIWGVEESVAHIGIYGAAS"
     gene            complement(4106..4885)
                     /locus_tag="PK242_06195"
     CDS             complement(4106..4885)
                     /locus_tag="PK242_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOZ93643.1"
                     /translation="MTVHYMNRGPAAWIVLDRPEALNALDFGMLEELAQAMQRADADP
                     DARVVVLTGQGRAFCAGGDIAQLRADMEAAPGQPSRYVDAVERGFDAVRALSKPLVGC
                     VNGVAVGGGLELLLCCDLVIAARTARIGDGHANYGIFPGGGSSALLPRALPINLARHL
                     LFTGALWPAARWHELGLVNQLADLTALEQETQTLAEDLARKSPRLLARLKQTINTCLD
                     KPLAQALRDELGELRSHMQSADMREGTSAFLEKRTPLYSGN"
     gene            complement(4890..6092)
                     /locus_tag="PK242_06200"
     CDS             complement(4890..6092)
                     /locus_tag="PK242_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ93644.1"
                     /translation="MTATGKSRLSQALVGLSLAVLAHGAQAQDSGITDKEILIGEVLM
                     LSGPAAFIGKSAQVGSKLAAAEINAAGGVLGRQLKVLYEDDGYVPSRSVSSVRKLIDV
                     DKVFAITGTTGGSGVTAIMPVIEQASVPTIVHVAPNAAVLNPRKPTVFMIGPDYDVAA
                     YEGLRYLAEKQGKKGAKFAVLYQNDDYGKGVLRGFQKAVKELGLNAVAEVPYQRGARD
                     FSAEALRLQQAGAEVLYLGTTVTEPGGILTETRRLGMDMTVIGNWAAGLSATIRLAAP
                     AGYDYFFNDYYAALNDPAGKRLVESAQKHLSADEQASLSRYSISGYVGIRVMAEAIKR
                     CDKRLTRACVTEQLGKLKDFATGGLSGPISLDNPQGHAVPPLKMFQVNAKDGSLRAVT
                     DFMPYTSR"
     gene            complement(6127..7173)
                     /locus_tag="PK242_06205"
     CDS             complement(6127..7173)
                     /locus_tag="PK242_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ93645.1"
                     /translation="MRSGTFKENYAGLIVLTDNPVKWVWAAVALTLVAVFPLVANSYT
                     LNLATMVAVAGVAVIGLNLLTGIAGQLSLGHAGFMAVGAYAQAILTTDYGWPGLLAIP
                     VAGVLAAISGLVVGVPSLRLRGLYLALTTLGFSYIVTHILVEWESVTRGPSGMPAEAL
                     RIPGTDLGPDQAVYAVAWSVLVLAVLASLNLNRSRIGRAWHALHEHDIAASAMGINLT
                     AYKLQAFLASSFFAGCAGALMAGQTRYVNVDSFSILLSVEMLAMLIVGGIGRVSGAVL
                     GAAFIVMLPELLRWGVDGVGAGLQALLANHAPETKELLYGAVILLFLRFEPEGLAGIW
                     RKFKRFFVNWPLSR"
     gene            complement(7173..8045)
                     /locus_tag="PK242_06210"
     CDS             complement(7173..8045)
                     /locus_tag="PK242_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ93646.1"
                     /translation="MDLLLNLTAAGLIVGAVYALLAMGFAVVYRATGLLNFAQGDVML
                     LVAYTSYAVASAGQWGFVATLATALVTGVVVGVLLEFIFIRPMAGESMFSKVMVTIAL
                     AIMIRAAVVMVAGAEPLPMPRLLGSALLRVGPISLYAAQLATLGLTVLCCLATWMLFN
                     HTRVGISMRAAACDEGAALLAGIAVRRVHALAWAMAGLYAGLAGMGCALMFNVDPTLS
                     SLGLRAFPAAILGGLDSVLGSALGGLLIGLLESYAGGYLGRGMKDVIGFAMIVVVLMV
                     KPYGLFGVRRIERV"
     gene            complement(8045..9949)
                     /locus_tag="PK242_06215"
     CDS             complement(8045..9949)
                     /locus_tag="PK242_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOZ93647.1"
                     /translation="MTSSIRHPLAALHPSVPARAATLPALLRQRASEHPDRLALRHKD
                     RGIWRRVSWAEYDAQVMHLALFLRARGLGVGDCVAIVGDGSPGWFFADLATQSLGGIA
                     VGIYPTNPWAELQYIVRHCRARVVVCGDQEQVDKVLDALREEGGLPHLELILVVDPKG
                     LRHYEEERLVVLDDAIAEGARLAGAVPEKLRALHADVQALRPEQDAIIVYTSGTTGMP
                     KGARISHRSVITASWRLGQVYGFEGRALSALCYLPLCHVAERLFSTVTQLVWNGVVNF
                     AESIDTVTADLREIAPSYFLGVPRIWEKIQRDIMLASRQSHPLARTAFGWALGRAERL
                     AHAANERPGQHLPWQQRLEGRVLQALFFNNLLAGLGLDRMFFAAAGGAPVPAHTTVFF
                     QALGLLVNEVYGMTETCGLINVQRPGSTRLGWAGPPVEGIEQCTGPDGELLVRGDMVF
                     SGYLHDEAATQAAMEGGWLHTGDIAERDAATGDIRIVDRKKSIIITSGGKNITPSLIE
                     NALKESPYIEEAILLGDGRHFLAALIQIDLVTVGHWAQEQGLAYTHYAHLASLPEVHE
                     LVDAAVQAVNGRFARVENVRKFVILRKQLDHDDGEVTATMKVRRAAIEKKFRAEIEEI
                     YGPAKQEETA"
     gene            complement(9946..10752)
                     /locus_tag="PK242_06220"
     CDS             complement(9946..10752)
                     /locus_tag="PK242_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ93648.1"
                     /translation="MSAVHQLHGTPGSAMLSIQGLNKRFGGVQALTNVSMQVLPGEIC
                     SVIGPNGAGKTTLFNIISRFFREDEGELRLQGQALRALRPHQMSAQGVARTFQNLAIF
                     KHRTVVENILVGMHARLRTGMLAAAAFLPSVRRTEIAAREEVEEIIEFLEIEALRDQP
                     VGSLSYGLQKRVELGRALAVKPRLLLLDEMVSGMNQEETEDIARFILDIREQRGTTVM
                     MIEHEMGIVMDISDRVVVLNFGHKIAEGTPTEVAADPAVIDAYLGQRRAA"
     gene            complement(10749..11513)
                     /locus_tag="PK242_06225"
     CDS             complement(10749..11513)
                     /locus_tag="PK242_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ93649.1"
                     /translation="MYDGVVEGVRDVSLSVERQRIVALLGANGAGKSTVLKAVSGILY
                     TEDGAITDGSIQFDDQPIDRLGPQAIVRAGLVQVPEGRRLFDTLTVNENLLAGGAALR
                     RDALRQTLDQVFDLLPELADHRGRVAGLLSGGQQQMVAIGRALMAKPRLLMLDEPSLG
                     LAPQVSDKILGAVRRLRDEAGLTVLLVEQNARAALELADYGYIMDRGRVVLDGESTAL
                     LRNDDVREFYLGLGAQGQRRSMRDVKHYRRRKRWLA"
     gene            complement(11974..>13073)
                     /locus_tag="PK242_06230"
     CDS             complement(11974..>13073)
                     /locus_tag="PK242_06230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOZ93650.1"
                     /translation="KLADWLAPLLAPRGRQVAWLTGGQKKKARADMLAAIESGAAALV
                     IGTHAVIQQQVRFARLGLAVIDEQHRFGVQQRLDLRNKLSEAEQEPHLLMMSATPIPR
                     TLAMSYYADLDVSTLDELPPGRTPVITRLVAQARRGEVIERIRAQIAQGRQVYWVCPL
                     IEESEALDLRHASATHAELAAALPGQAVGLLHSRLPPADKQAVMQQFVSGAMPVLVST
                     TVIEVGVDVPNASLMVIEHAERFGLSQLHQLRGRVGRGAAASACVLLYATPEGGRLGD
                     SARERLKAMVETSDGFEIARRDLAIRGPGEFLGARQSGDALLRFADLAEDAPLLAWAR
                     RAAPRLLDHWPERAARHVDRWLGGRMDYLKA"
BASE COUNT         2324 a         4476 c         4093 g         2180 t
ORIGIN      
        1 tgccgctcgc cacgccggag cagttcaagg ccacggtggc caaggatctg caggattgga
       61 cggccctgat caagtcgatc ggtctgacgc tggactaacc tagtacatca acacgtaaat
      121 aacggaacgt aatggcgccc gagcttccga tccgcatcct gtcgagtgaa cttccactcg
      181 atggtttgct tggcagcgtt gcgagcgagc tgccactgct cgacttcgcg ccgcagagtg
      241 tggcggtcgg cccagcgacg atccaggcat tggcggctga ggatgccgat ttcgatctcc
      301 gccatgttca gccagctcgc gtgcttgggt gtgtagtgaa attgcacgcg ccgcagcaac
      361 ttggcggccg cgcggcaccc cagaacatcg tcgaagcatt tcctgaagtg gatgttcagg
      421 ttatccaaca cgaggtggac ccggcgcgcc ctggcgtagg cgccaccgag cagcgaggcg
      481 atgaattcga cgaagtcgac cttgccacgc tgcgcggtga ccgcgatcaa gcgctgtcct
      541 gccttgggct cgacggcgac gaacaggttg ctggtgccgc gccggacgta ctcgtagtcg
      601 tccttggcga ggatgccggg cgacatcggc aatggcgcac gactgtgacc gatcagctgc
      661 agactcttct cgtcgatgca caccacgggt tcgcgtggat cgaacggttg ggcgtacagc
      721 gccaacagcg cgtacgtgcg gtgacggtac tcctcggtca gctcgccgat gcaccacatc
      781 agccgccgcc agggtttgag gtcgtttttt tgagcatgcg gcgcaccgtc tcgcgactca
      841 aattgcccag cgctggctcg cgccgggcgg cgcgctcgag ctcgaccatc gtccagcgct
      901 tgcgtccttc gggcggcgcc gagcacgcca gcgccgtgac ttgcgcctcc acgtccgtgt
      961 cgtacgtgcg cgggcggccg gggcgggtca cgtcgaacag cgcgagttcc aggccgcctt
     1021 gcaagtacgc cgcccgtgtg cgccacaacg ccgtgcgtcc cacacccagt accgccagga
     1081 tctgtgcctc cggcacgccc cgatccaggc acgccagcat gtgcgcccgg tggatctccc
     1141 gactgcgatg aagccccttg ctgcggacct cttcgaccac cgcgcggtct tcctcgctca
     1201 aatgcaattg cgtctggcgc atgaatacac ctccgtcggt cagatgacga cttgcaaagt
     1261 gtattcatgt ttcgttattt atgtgtcgtg atactagtgt ccgacgccgg tcacgtcaaa
     1321 ggcttgtcgc ccggctttcg actgcgtctg gcatttcccg cgccgggggg cgcctgtacc
     1381 ccatgaagca gcagaaacag gatctgatcc gcggtgcttt cgacatccag cgaaccttcc
     1441 cccatcaggt agcgcgtggc gtggtgctcg atgcccccga agatcatgtc gcgcaccagc
     1501 ctgacaggaa tgtccctgcc gaactcgcca gattcgaccc cgcactcgat ggcgtgacgc
     1561 aggtgccacg tatagcgctg gttgagtttg ccgacgatgg cgcgaaactc ggtatcgctg
     1621 gtgcgcagtt cgcgaatgat caggcgcatc agaccgggac tgtcccgcaa ggcgcgcaga
     1681 tgcgccgcaa tcagaaagcg caagcggctg cgtaccgacg tgatatgccg caactcgttc
     1741 tcgagctccg cattcgtgcg gctgtaccac tcggaaatca cgtgtgcgag caactcgcgc
     1801 ttggtcttga cgtagcgata gacgttgccc tccaccacac cggcccgccg cgcgacttcg
     1861 gccaccgtgg cagagtcata gcccatctcg ctgaacacct gctcggccac caccaggatt
     1921 tcctcccagc gctcctcctt ggaaaggcgc ctgcgccgtg cgggcgccga agcgacaggg
     1981 ttggcgtcgg ccgaacggcg gggtgaacgg gcaactcgcg acatgtctgg tgacgtgggg
     2041 ggggatcaac ccagatggcc tgcggacggc aaccatccta ccgagggggc agtcgaaatg
     2101 ctccgtcgag ccggatgcat tcgccgttga tgaagtgatt ctcggcgata tgacagcaca
     2161 aggcggcaat ctcctctgct cgtccaaagc gccgcggaaa ctgaaaatcg gcttgaatgc
     2221 gctccaacac cgtttccggc aagctctcca gcatgggcgt ccagaacgcg cccggagcaa
     2281 tggtgttgat ccgaatgccg atccctgcca gctcgcgcgc cgctggcagg cacagaccca
     2341 ccagtccggc tttgctagac gcataggcgg tctgcccgac ctggccatcg aaggccgcgc
     2401 ccgaggcaat ggtgacgagc acgccgcgct ccccgtcgtc gtctggcggg ttgcgctgca
     2461 tctgctgcgc gcagtgagac agcagatgaa aggtccccac caggttgatg gccacggtgc
     2521 gagcgaacgt cgcgcccgag ctggccttgc cgtctcgatc cagcatgcgc atcggtgaaa
     2581 ccacccccgc aaagctgaag caggcatgca gggaaccgaa gtgttcgacg gcccgctgca
     2641 cggcccctgc cacctgcgcc tcatcggcca gatccgcctg cacgaacagg catcgacctg
     2701 ggtccaattc cgccgcccgc gccgcgccct gttcagcgtt gcgatccagc atcaccacgc
     2761 gtgcgcctgc ctcgcgcaca cagtactggg cagcgcccca cccgaggccg gatgccgcgc
     2821 ccgagatcag caccacggcg tcccgcattc tcatgatgcg gctccataga tcccgatatg
     2881 ggccaccgac tcctcgacgc cccagatgcc gccggcgttc tcctgcacgg ccagtcgcgc
     2941 cccctgaacc tggcgcgagc cacactcacc gcgcaactgt gcgaccagct cgaaaatctg
     3001 ccccagcccg gtggcgccaa tgggatgtcc tttgctctcc agtccaccgg aagggttcac
     3061 cgggatgcgg ccgccgaggg ttgtctcgcc gcgctcggcc agctcgccgc cctcgcccag
     3121 gccgcagaat cccaggttct cggtctgcat cagctctccc atggcggtgg cgtcatgcac
     3181 ctcggccagg tgcatgtcgc ccgggcccat gcccgccgcc tcataggcct gctgcgcagc
     3241 caggcgggtc aggtgatgct ccaggtcttg cgcgtcgcgc gcagtggccg tgcgcagcac
     3301 gctggcgtgc acgatgacag cgcgccgcct gagcccttgc aggcgcgcca gacccgccgg
     3361 agtgcacaac agggcagcgg cggcgccgtc ggaaatgggt gcgcacatgg gcaatgtcaa
     3421 tgggtaggtg atgggaggcg aggcaagcac ctcctccacc gtcatggcct ggcggaactg
     3481 ggctcgctca ttggcaacgg cgtggttgcg gtttttcgca gcaatggccg ccaactgacg
     3541 ctgcgtgatg ccgaagcgct ccatgtattg acgcgcaaac ccggcgtaga catccatgaa
     3601 ggggctgtac tgcgccgggg aaacggatcc ttcggggatg gcgacaccct tgcccagcgc
     3661 cgtgaagtgc gcatggttct gcgcgggcgt gtccacatcc caggcggcgt caaagatggc
     3721 gaaccggcgc tcgcgctcgg cggtgaacag cttgtccacc cccaccgcca gcgcaatgtc
     3781 gcaggcgccc gagcgcaggt agtgaaaggc catgtgcaac gccgtgctgg cggtagcgca
     3841 ggcgttttcc acattgatga tgggtatgcc ctgcagcgcc agaggcagca gcgccatctg
     3901 gccgcgtacc aggtgctggc catcgagcag cccctgcgca cagttgccat agaaggccga
     3961 ctggacttgc gccggctcac agccggcatc ggccagcgca tcgcgcacag cccattcgac
     4021 cagttgcttg gccgtcaatt cggggtgacg ggcgaacggc gtcatgccca cgcctacgat
     4081 gagaacttgc gtcatgtgaa tacgatcagt tgccggagta gagcggagtg cgcttttcca
     4141 ggaaggccga ggtgccttcg cgcatgtcgg ccgactgcat gtgactgcgc aactcgccaa
     4201 gctcgtcgcg caatgcctgg gccaggggct tgtccaggca ggtgttgatg gtttgcttga
     4261 gtcgcgccag cagacgcgga ctcttgcggg ccaggtcttc ggccagggtc tgggtttcct
     4321 gttcaagcgc cgtgagatca gccagctgat tgaccaggcc cagttcgtgc caccgcgcgg
     4381 caggccacag cgccccggtg aacaacagat gccgtgccag gttgatgggc agcgcccgcg
     4441 gcagcagggc cgaagaacct ccgccaggaa agatgccgta gttggcatga ccatcaccga
     4501 tgcgtgccgt tcttgccgcg atcacgaggt cgcagcacag cagcaactcc aacccacccc
     4561 cgacggccac gccattgaca cagccgacca ggggtttgct gagtgcacga actgcgtcaa
     4621 aaccgcgctc cacggcgtcc acataccggc tgggttggcc cggtgcggcc tccatgtccg
     4681 cccgcagctg ggcaatgtcc ccgccggcgc agaaggcgcg gccctgaccc gtcaggacca
     4741 ccacgcgggc gtccggatcg gcgtctgcac gctgcatggc ctgcgccagc tcttccagca
     4801 tcccgaaatc cagcgcattg agcgcctcgg gacggtccag gacgatccac gccgccggac
     4861 cgcggttcat gtaatgcact gtcatgaggt taccggctgg tgtagggcat gaagtcggtg
     4921 accgcgcgca ggctgccgtc cttggcgttg acctggaaca tcttgagcgg cggcaccgca
     4981 tggccctgtg ggttatccag cgagatcggg ccactgagac cgccggtggc aaagtccttg
     5041 agctttccaa gctgctcggt cacgcaagcg cgcgtcaggc gcttgtcgca gcgcttgatg
     5101 gcctctgcca tgacgcggat gcccacgtaa ccactgatcg aatagcggct cagcgaggcc
     5161 tgctcatccg cgctcaggtg tttctgggcg ctttccacca ggcgtttgcc cgccgggtca
     5221 ttgagcgcgg cgtagtagtc gttgaaaaag tagtcatagc cagccggcgc agccaggcgg
     5281 atggtggccg acaaccctgc cgcccagttg ccgatcacgg tcatgtccat gccgagccgg
     5341 cgcgtctcgg tcagaatgcc gcccggttcg gtcacggtcg tgcccagata gagcacttcg
     5401 gcccccgcct gctgcaggcg cagtgcctcg gcgctgaaat cgcgggctcc gcgctggtaa
     5461 ggcacttcgg ccactgcatt cagccccagc tccttcaccg ccttctggaa gccacgcagc
     5521 acgcccttgc catagtcgtc attctggtag agcacggcaa acttcgcgcc ctttttgccc
     5581 tgcttctcgg caaggtagcg cagtccttcg tacgcagcga cgtcgtagtc ggggccgatc
     5641 atgaacacgg tgggcttgcg cgggttgagc acagcggcgt tgggcgccac gtgcacgatg
     5701 gtcggcaccg aagcctgctc gatcaccggc atgatggcag tcacgcccga accgcccgtg
     5761 gtaccggtga tggcaaacac cttgtccacg tcgatcagct tgcgcaccga tgacacggag
     5821 cgagagggca cataaccatc gtcttcgtac agtaccttca gctggcggcc cagcacgccg
     5881 ccggcggcat tgatctcggc ggcggcaagc ttggagccaa cctgggccga tttgccaatg
     5941 aaagctgccg gcccggaaag catcaggacc tcgccgatga gaatttcctt gtcggtaatg
     6001 ccactgtcct gcgcctgcgc gccgtgcgcg agcacggcca aggaaagacc caccagggcc
     6061 tgactcagac gcgattttcc tgttgcagtc atgcttgtct ccttcagtga ttgaatgggt
     6121 ggccgctcaa cgcgatagcg gccagttcac gaaaaaccgc ttgaacttgc gccagatacc
     6181 ggcaagtcct tcgggttcga agcgcaggaa cagcaggatc actgctccgt acagcaactc
     6241 cttggtctca ggggcgtggt tcgccagcag tgcttgcagc cccgcgccca cgccgtccac
     6301 accccatcgc agcaactccg gcaacatgac aatgaaggca gcgcccagca cagcacccga
     6361 gacgcggccg atgccgccca cgatcaacat cgccagcatc tccacactga gcagaatgct
     6421 gaaactgtcc acattgacgt agcgcgtctg cccggccatc agcgcgcccg cacagccggc
     6481 aaaaaacgag ctcgccagga acgcctgcaa cttgtaggcc gtgagattga tgcccatcgc
     6541 actggccgcg atgtcgtgct cgtgcagcgc gtgccatgcc cgcccgatgc ggctgcggtt
     6601 cagattcagg ctggccagta ccgccagtac cagcacgctc caggccacgg catacaccgc
     6661 ctgatcaggc cccaggtcgg taccgggaat gcgcaacgcc tcggcgggca tgccgctcgg
     6721 cccccgggtc acgctttccc actccaccag aatatgcgtg acgatgtagg aaaaacccag
     6781 cgtggtcagc gccaggtaga gtcccctcaa acgcagcgac ggcacgccaa ccaccaggcc
     6841 tgaaatcgcc gccagcacgc ctgccacggg tatggccagc agtcctggcc aaccgtaatc
     6901 ggtggtcaga atcgcctgtg catacgcacc aactgccatg aagcccgcat gacccagcga
     6961 gagctggccc gcgatgcccg tcagcaaatt caggccgatc accgcaaccc cggcaaccgc
     7021 aaccatggtg gccaggttga gcgtgtagct gttggccacc agcggaaaga ccgccaccag
     7081 ggtcagcgcg accgccgccc aaacccattt caccgggttg tccgtgagga cgatgagccc
     7141 ggcgtagttt tccttgaacg ttccgctgcg catcacaccc tctcgatccg gcgcacgccg
     7201 aacaggccgt atggtttgac catcagcacc accacaatca tggcgaaccc aatcacgtct
     7261 ttcatgcccc gtcccaggta gcctccggcg tagctctcca gcaggccgat cagcagcccg
     7321 cccagagctg acccgagcac cgagtccagg ccgcccagaa tggccgccgg gaaggcgcgc
     7381 agccccaggc tggagagcgt tggatcgacg ttgaacatca acgcgcagcc catgcccgcc
     7441 agccccgcgt aaagacctgc catcgcccag gccagtgcat gcacccgcct gacggcaatg
     7501 cccgcgagca gcgccgcgcc ttcgtcgcag gccgcagcgc gcatcgaaat acccacccgc
     7561 gtgtggttga agagcatcca ggtcgccagg cagcacagca ccgtcaaccc cagcgtcgcc
     7621 agttgcgccg cgtacaggct gatcgggccg acgcgcagca aggcactgcc caacaggcgc
     7681 ggcatgggca gcggctcggc accggcgacc atgacgacgg cggcacgaat catgatggcc
     7741 agggcgatgg tcaccatcac cttggagaac atcgattcac cagccatggg gcggatgaag
     7801 atgaactcca gcagcacgcc gaccaccacc cccgtgacca gggccgtggc cagcgtggcc
     7861 acaaagcccc attgaccggc actggccacc gcgtagctgg tgtaggccac caggagcatc
     7921 acgtcgcctt gggcgaagtt cagcaagcca gtggcgcggt agaccactgc aaagcccatg
     7981 gccagcagcg cgtagacggc acccacaatc aggcccgccg cggtgaggtt gagcagcaga
     8041 tccatcaggc cgtctcctct tgcttggcgg ggccgtagat ctcttcgatc tcggcgcgga
     8101 acttcttttc gatggcggcg cggcgcacct tcatggtggc cgtcacctcg ccgtcatcgt
     8161 gatcgagctg cttgcgcagg atcacgaact tgcgcacgtt ctccacccgg gcaaagcgtc
     8221 cattgaccgc ctgcacggcg gcatcgacga gctcgtgcac ttcgggcagt gaggccaggt
     8281 gcgcgtagtg ggtgtaggcc agaccctgct cctgcgccca gtgacccacg gtgaccaggt
     8341 cgatctggat cagcgcggcc aggaaatgcc ggccatcacc caacaggatc gcctcctcaa
     8401 tgtaagggct ctccttgagc gcgttctcga tcagcgaggg cgtgatgttc ttgccgccgc
     8461 tggtgatgat gatcgacttc ttgcgatcga cgatacggat atcgcccgtg gccgcgtcgc
     8521 gctcggcgat gtcgcccgtg tgcagccagc ccccctccat ggccgcctgg gtggcggcct
     8581 cgtcgtgcag gtaaccgctg aagaccatgt ccccgcgcac cagcagctcg ccatcggggc
     8641 cggtgcactg ctcgataccc tcgaccggcg gcccggccca ccccagccgc gttgagccag
     8701 ggcgctgcac gttgatcagg ccacaggttt cggtcatgcc gtagacctcg ttgaccagca
     8761 gccccagtgc ctggaagaac accgtggtgt gtgcgggcac tggcgcacca cccgcggcag
     8821 caaagaacat gcgatcgagc cccaggccag ccagcagatt gttgaaaaac agcgcctgca
     8881 acacgcgacc ctccaggcgt tgctgccagg gcaaatgctg accagggcgt tcgttggcgg
     8941 catgcgccag acgctctgcc cggcccagcg cccagccgaa cgccgtgcgc gccagcgggt
     9001 gcgactgccg cgaggccagc atgatgtcgc gctggatctt ttcccagatg cgcggcacgc
     9061 ccagaaagta cgaaggtgca atctccctca gatcggccgt caccgtgtcg atggactcgg
     9121 cgaagttgac cacgccgttc cagaccaatt gcgtcacggt ggaaaacagg cgctcggcta
     9181 cgtggcacag cggcaggtaa cacagcgccg acagggcacg gccctcgaat ccatagacct
     9241 ggcccagccg ccaggacgcc gtgatgacgc tgcggtggct gatgcgagcg cccttgggca
     9301 tgcccgtggt gccggaggta tagacgatga tcgcgtcctg ttcggggcga agcgcctgga
     9361 catcggcgtg cagagcacgc agtttttcgg gcaccgcacc cgccaggcgg gcgccttcgg
     9421 caatggcgtc gtccagcacc accaggcgct cttcctcgta gtgccgcaga cctttgggat
     9481 ccacgacgag aatcagctcc aggtgtggca gaccgccctc ttcacgcagg gcgtccagca
     9541 ccttgtccac ctgttcctgg tcgccgcaga ccacgacccg ggcgcggcag tgacgcacga
     9601 tgtattgcag ttcggcccag ggattggtcg ggtagatgcc cacggcgatg ccgcccagcg
     9661 actgggtcgc caggtcggcg aagaaccagc cggggctgcc gtcaccgacg atggcgacgc
     9721 aatcgcccac acccaggccg cgcgcgcgca gaaaaagcgc caggtgcatc acctgggcgt
     9781 cgtactcggc ccagctcaca cgccgccaga tgccacgatc cttgtggcgc agggccagcc
     9841 tgtccggatg ttcgctcgca cgttggcgca gcagggccgg cagcgtggcg gcacgtgcag
     9901 gcacgctggg gtgcaacgcc gccagcgggt gacggatgga tgatgtcatg ctgccctccg
     9961 ctggcccaga taggcgtcga tcaccgcagg gtctgccgcc acctcggtgg gcgtgccctc
    10021 ggcgatcttg tggccgaagt tgagcaccac cacgcggtcg gagatgtcca tgacgatgcc
    10081 catctcgtgc tcgatcatca tcaccgtggt gccgcgctgc tcgcggatgt cgaggatgaa
    10141 gcgggcaatg tcctcggttt cttcctggtt catgcccgag accatttcat cgagtagcag
    10201 caggcgcggt ttcaccgcca gggcgcgccc cagctccacg cgcttttgca atccgtacga
    10261 aaggctgccc acgggctgat cgcgcagcgc ttcgatctcg agaaactcga tgatctcttc
    10321 cacctcctcg cgtgccgcga tctcggtgcg acgcaccgac ggcagaaagg ccgccgccgc
    10381 cagcatcccc gtgcgaaggc gcgcgtgcat gcccaccaga atgttctcca ccacggtacg
    10441 gtgcttgaag atggccaggt tctggaaggt gcgcgccacg ccctgcgcgc tcatctgatg
    10501 cggcctgagg gcgcgcagcg cctggccctg tagcctcaac tcgccctcat cttcccgaaa
    10561 gaaccgcgag atgatgttga acagcgtggt cttgcccgcc ccgttggggc cgatgaccga
    10621 gcagatctcg ccgggcagca cctgcatgct gacgttggtc agcgcctgca cgccgccaaa
    10681 gcgcttgttc agcccctgga tggacagcat ggcgctgcca ggagtgccgt gcagttgatg
    10741 gacagcgctc atgcgagcca cctcttgcga cggcggtagt gcttcacatc gcgcatgctg
    10801 cgccgctgcc cctgcgcccc caggcccagg tagaactcac gcacgtcgtc gttgcgcagc
    10861 agcgcggtgg attcgccgtc gagcacgaca cgccctcggt ccatgatgta gccatagtcg
    10921 gccagctcca gcgcagcgcg cgcgttctgc tcgaccagca gcaccgtcag gcccgcctcg
    10981 tcacgcagcc tgcgcacggc ccccagaatc ttgtccgaca cctgcggcgc caggcccagc
    11041 gagggttcgt cgagcatcag cagccggggc ttggccatca gcgcccggcc gattgccacc
    11101 atctgctgct gaccgcccga cagcaaaccg gccacgcgcc cgcgatggtc tgccagctcg
    11161 ggaagcagat caaacacctg atccagcgtc tggcgcaagg catctctgcg cagggccgca
    11221 ccacctgcca gcaggttttc attcaccgtc agggtgtcga acaggcgcct gccctcgggc
    11281 acctgaacca gccctgcgcg cacgatcgcc tgcgggccca gtcggtcaat gggttgatcg
    11341 tcgaactgga tggaaccatc ggtgatggcc ccgtcttccg tgtagagaat gcccgacact
    11401 gccttcagca ctgtcgactt gcccgcgccg ttggccccca gcagggccac gatgcgctgc
    11461 cgctccacgc tcagcgacac gtcgcggaca ccttccacca cgccgtcgta catgacacga
    11521 agattggtga tgctcagcat attaatgagt tgaccacatt agtgatggag gcgatcaatc
    11581 ccatttgatg cctgtgaaat ttctgcctga ctgcccatga tgaaccaatg gattgcattg
    11641 ttagtgaggt tgactcatta tgaaactggg acaaaccctt ggttcagtcc aaaccccctg
    11701 gggcaacgac tccatcaagc agcgatcgct ggatgcgtcc gcctgagccg tgaccggcgt
    11761 gccgtgcgaa tgcgaacgct gtggttccaa tggcgctcgc gcgccttcgg gcaacgtgaa
    11821 cggggtcggc ctctggacaa gtcgcgccct gcaaggtgac gccgtcgcag gtggttgtcc
    11881 acaacaaggg atcgaacaca agcccggcct gcattccgag ccatggggca cgaggccgaa
    11941 ttgccgctgg cgccggtcca cccggcaggt ggctcaagcc ttcaggtaat ccatccgccc
    12001 gcccagccag cggtccacat gccgcgcggc ccgttcgggc cagtggtcga gcaggcgcgg
    12061 cgcggcgcgg cgggcccagg ccagcagcgg tgcgtcttcg gccaggtcgg caaaacgcag
    12121 cagggcgtcg cccgactggc gtgcgcccag gaattcgccc gggccgcgga tggccagatc
    12181 gcgccgggcg atttcgaaac cgtcgctggt ttcgaccatg gccttgaggc gctcgcgcgc
    12241 gctgtcgccc agccggccgc cttcgggcgt ggcatacagc agcacgcagg ccgaagcggc
    12301 ggcgccgcgc ccgacgcgcc cgcgcaactg gtgcagctgg ctcaggccaa agcgctcggc
    12361 gtgctcgatc accatcaggc tggcgttggg cacgtcgacc ccgacctcga tcacggtggt
    12421 ggacaccagc accggcatgg cgccgctgac gaattgctgc atcaccgcct gcttgtcggc
    12481 cggcggcagg cgcgaatgca gcaggcccac cgcctgcccc ggcagcgcgg ccgccagctc
    12541 ggcgtgggtg gcgctggcgt ggcgcaggtc cagcgcttcg ctctcttcga tcagcgggca
    12601 cacccagtag acctgccgac cctgagcgat ctgggcacgg atgcgctcga tcacttcgcc
    12661 gcggcgcgcc tgggccacca gccgggtgat gaccggcgtg cggccgggcg gcagctcgtc
    12721 gagggtggag acgtccaggt cggcgtagta gctcatcgcc agggtgcgcg ggatgggggt
    12781 ggcgctcatc atcagcaggt gtggctcctg ctctgcttcc gatagcttgt tgcgcaagtc
    12841 cagcctttgc tgcacgccga aacgatgctg ctcgtcgatg accgccaggc ccagccgggc
    12901 aaagcgcacc tgctgctgga tgacggcgtg ggtgccgatc accagcgccg ccgcgccgga
    12961 ttcgatggcg gccagcatgt cggcgcgtgc cttcttcttc tgcccgccgg tcagccaggc
    13021 cacctgccgg ccgcgcggcg ccagcagcgg cgccagccag tcggccagct tgg
//
LOCUS       DAOOOG010000060        12865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_5359_length_12865_cov_25.898985, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000060 DAOOOG010000000
VERSION     DAOOOG010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_12865_cov_25.898985"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /gene="ilvN"
                     /locus_tag="PK242_06235"
     CDS             complement(<1..176)
                     /gene="ilvN"
                     /locus_tag="PK242_06235"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOZ93651.1"
                     /translation="MKHIIAVLIENEAGALSRVVGLFSARGYNIESLIVAPTEDPSLS
                     RMTIQTRGSDEVIE"
     gene            complement(306..2093)
                     /locus_tag="PK242_06240"
     CDS             complement(306..2093)
                     /locus_tag="PK242_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HOZ93652.1"
                     /translation="MEISKAELKSAAVAASADTSQAPQELRGAEILIKALQAEGVKYV
                     WGYPGGAVLHIYDAFYKQDTIQHVLVRHEQAAVHAADGYARATGDVGVALVTSGPGVT
                     NAVTGIATAYMDSIPMVIITGQVPTAAIGQDAFQECDTVGITRPIVKHNFLVKDVRDM
                     AEVMKKAFHIARSGRPGPVVVDIPKDVSFEKTAYKGYPQSVEMRSYNPVRKGHGGQIR
                     KALQLLLAARRPYIYTGGGVLLADACAELRTLVDLLNCPVTHTLMGLGAFPASDRRFL
                     GMLGMHGTWEANNAMQHCDVLLAVGARFDDRVIGNPKHFAQNERKIIHIDIDPSSISK
                     RVKVDVPIVGDVKDVLTEMIGMIREAGARPDEAALADWWKTVEGWRARDCMKYDRANT
                     DVIKPQKVIETLWEMTRGADAYITSDVGQHQMWAAQYYRFDEPRRWINSGGLGTMGVG
                     IPYAMGIKLAKPESEVYCVTGEGSVQMCIQELSTCLQYNTPIKIISLNNRYLGMVRQW
                     QELQYAGRYSHSYMDALPNFVKLAEAYGHVGMLIERPQDVEPALREARKLKDRTVFMD
                     FRTDPTENVFPMVEPGKGITEMLLGSEEL"
     gene            2395..2961
                     /locus_tag="PK242_06245"
     CDS             2395..2961
                     /locus_tag="PK242_06245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOZ93653.1"
                     /translation="MATDQELSDFLRNVEKRAFKRTVYHVRDDDAALDIVQDSMMKLA
                     EHYGDKPAAELPLLFQRILSNSTLDWFRRQKTRNALFSNMSDFDAEDGDGEFDLLEIF
                     AGASDSTQIESAEDATRRAQVLHEIELQIQALPNRQREAFLLRYWEEMDVAETAAVMG
                     CSEGSVKTHCSRAVQALAKALRAKGIQP"
     gene            2958..3425
                     /locus_tag="PK242_06250"
     CDS             2958..3425
                     /locus_tag="PK242_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HOZ93654.1"
                     /translation="MNTNFSQHHTESLRSRFGLRVGARLSAGTHELPHDVRERLRAAR
                     ERALAQRRQPVTQRVARPSWAAVRQGATLTLGAGDNGAGFWGRLASAALMLVLVAGLV
                     AIDVVQDNDRATEVAELDAALLTDDLPPEAYADPGFLQFIKSDLGRTSSTLSQ"
     gene            3458..4078
                     /locus_tag="PK242_06255"
     CDS             3458..4078
                     /locus_tag="PK242_06255"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HOZ93655.1"
                     /translation="MRHFLPVLLLALTALTLWPAHAQEVAKPGSAAAVGGQIEWRSLS
                     TAQRTALAPLERDWASLGSDQQRKWIALTQNFSRMTPQERATLQGRMTEWANLTPAQR
                     TQARLNFGEVRKVPTDERRAKWEEYQALPEEERARLARERPKPPAGAAPALRPVPAER
                     LVRPARPPVPVVLPEAPASAPVPARVNRNTLLPQRPRPAAPNTESR"
     gene            4139..4687
                     /locus_tag="PK242_06260"
     CDS             4139..4687
                     /locus_tag="PK242_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOZ93656.1"
                     /translation="MNSSAATPSTPIAVAGSPLRAPSLRRRMACWLYEGTLMFGVVVL
                     PGLVFGIATNTRHALDNRHALQALLFVVLALYFVYFWVKGQTLPMKTWHIRVVDRHGL
                     PLRWPRALLRYLLAWLWFLPPLAAMAPFRLSGSEVTVLMFGWIAVWALLSRFQREQQF
                     WHDAWAGTRLIDVQPDAARRRQ"
     gene            4714..5127
                     /locus_tag="PK242_06265"
     CDS             4714..5127
                     /locus_tag="PK242_06265"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOZ93657.1"
                     /translation="MPPTDRPREQAPAHAHQRRTGLARLWHATGHSLAGLRAGWGEPA
                     FRQEALLALVLLPAALWLGRTWVEVALLVGSLLLVLMVELLNSGIESAIDRIGPEWHA
                     LSKRAKDMGSAAVLLSLLLGAGIWLAALYTHLFSS"
     gene            5124..5726
                     /locus_tag="PK242_06270"
     CDS             5124..5726
                     /locus_tag="PK242_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOZ93658.1"
                     /translation="MTAAAASEPAFSICVYCGSRPGEAQAFADVAAQVGHWIGRHGGQ
                     LVYGGGRAGLMGVVADATLAAGGRVVGIIPRVLVEREHAHRGCHELHVVETMHERKRQ
                     MAERADAFLALPGGIGTFEEFFETWTWRQLGYHDKPVGLLNQDGYYDAMLSFLQHSVA
                     SGFMGAAQMALIEVGTDWRALLGALVHATGFPAPDLLSQI"
     gene            complement(5743..6951)
                     /gene="hemW"
                     /locus_tag="PK242_06275"
     CDS             complement(5743..6951)
                     /gene="hemW"
                     /locus_tag="PK242_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOZ93659.1"
                     /translation="MAVNNDVRHYLRPGTLQLPALPPLSLYLHLPWCLKKCPYCDFNS
                     HERRTGVDARAQQGRYVDALLADLEASLPLVWGRPVYSVFIGGGTPSLFEPDDIDRLI
                     AGVRARLPLEPMCEITLEANPGTFERERFAAFRAAGVTRLSVGVQSFHDRHLKALGRV
                     HDGAQARAALQEAARAFDTFNLDLMYALPGQTLAELEQDLRTALDFAPPHLSIYHLTI
                     EPNTVFAKFPPRLPDEDTAWDMLDRITALTADAGLARYEVSAYARPGHRCVHNLNYWE
                     FGDYLGLGAGAHGKLSFPHRIVRQVRHRQPERYMEQALAGDAVAQAHEVARAELPLEF
                     MLNALRLKDGFSLTDYTERTGMPPSSIASALQQAEQSGWLQREGAWIRPTARGFDFLS
                     DLQQLFLPEG"
     gene            complement(6942..7541)
                     /gene="rdgB"
                     /locus_tag="PK242_06280"
     CDS             complement(6942..7541)
                     /gene="rdgB"
                     /locus_tag="PK242_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOZ93660.1"
                     /translation="MRIVLASNNPGKLAELQAMLAPLGMDLVRQAELGIGEAAEPFRT
                     FVENALAKARHAAAASGLPALADDAGLCVDAFGGLPGVDTAYYGTQFGYAKGDAGNVC
                     ALLEQMQGIDNRRAAMVSTLVALRRADDPEPLIAVGRVVGEIARAPRGSGGFGFDPVM
                     FIPEHGQTFAEMPTDTKNALSHRGRAVAAMVALMRERWL"
     gene            complement(7585..8322)
                     /gene="rph"
                     /locus_tag="PK242_06285"
     CDS             complement(7585..8322)
                     /gene="rph"
                     /locus_tag="PK242_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOZ93661.1"
                     /translation="MNASVSSPARTDRAPDQLRPVRITRHYTRYAEGSVLIEFGHTQV
                     LCTASVEEKVPPHKRGSGEGWVTAEYGMLPRATHTRGDREAARGKQSGRTQEIQRLIG
                     RSLRAVFDLTKLGERSILLDCDVLQADGGTRTAAITGAFVAAQDAVSGLLARGLIAES
                     PILHPVAAISVGIVGGVPMLDLDYAQDSSCDTDMNVVMTGAGHYVEVQGTAEGAAFSR
                     AEMDQLLRLAEKGIGELIEMQRRSLLN"
     gene            complement(8379..9287)
                     /locus_tag="PK242_06290"
     CDS             complement(8379..9287)
                     /locus_tag="PK242_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOZ93662.1"
                     /translation="MKFSVFQLSRRGGREKNEDRMGYSYTRDAGLFALADGMGGHPEG
                     EVAAQIALQTMSALFQREARPSLAVPTSFLASSLMGAHHQIIRYASERGLLDTPRTTL
                     VAALVQEGELHWMHCGDSRLYVVRDGQLLVRTRDHSYSEAQAGAGMTSEAINRNVLFT
                     CLGSTVRPMFDIAGPLRLMQGDRVLLCSDGLWGSLDDDLIVRTLGALPVAKAVPALVD
                     VALQNAGEHSDNVTVLAFEWESPPESAFEHSGFTETQVLSDEVFASTIQAGAYDPLLD
                     DLDDEAIERSIAEINAAIQRTAMNRR"
     gene            complement(9299..10306)
                     /locus_tag="PK242_06295"
     CDS             complement(9299..10306)
                     /locus_tag="PK242_06295"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOZ93663.1"
                     /translation="MSKAKPAPLPPDTEIGGYRVLRRISAGGFGVVYLAVDREGQQVA
                     IKEYLPSALTLREPGELVPQVAPEKLSLYRLGLKSFFEEGRSLAQISHPSVVSVLNFF
                     RQNETVYMVMNYLEGATLQDFIVTARELKKQKVFRESTIRSLFDEILRGLRIVHQHKM
                     LHLDLKPANIFITDDNKAVMIDFGAAREVLNKEGNFTRPMYTPGFAAPEMYRRDSTMG
                     PWTDVYAIGACIYSCMHGYPPNDAPQRLEKDRLSLSLTRMRGVYSDNLIEVVEWCMSL
                     DPLSRPQSVFALQKELSREGERQYTRLSVGERVRLQFDNMMNDTKQSLKRVTRFGARP
                     K"
     gene            10399..11319
                     /locus_tag="PK242_06300"
     CDS             10399..11319
                     /locus_tag="PK242_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOZ93664.1"
                     /translation="MSVYSMTGYASGQQALAGDAEGGSGAKNARGGVGMELRSVNSRF
                     LDLSFRLADELRPHEPALRELLGQRLRRGKVELRAWLQGQADDALAEPSPRLLQRLAT
                     LEDQVCNWLPQATRLSVAEVLRLAGGTDSLSDEAAAEALRALARRVLDDFVAAREREG
                     ARLARLIGERVTQLRALARAAEPLVPQAVQAQKQRFLDRWREALASGEGQIGAEAAQE
                     RALAEATAYAIRIDVAEELGRLAAHLDEIEAVLHQGGETGKRLDFLIQELHREANTLG
                     SKAAVLELTRISVDMKVLIEQMREQVQNIE"
     gene            complement(11472..11975)
                     /locus_tag="PK242_06305"
     CDS             complement(11472..11975)
                     /locus_tag="PK242_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HOZ93665.1"
                     /translation="MTRPSTPRRALLAALTLCGGAAFAAGSHHGPHGEHAATDSAIGQ
                     PGVAARASRSVRIDMNDRMRFSPERLAVRQGETVRLVVRNTGQVAHELSLGTLQDLLA
                     HRDQMARQPGMTHAEPNQITLAPGQQGEIVWHFSRAGTVHFACLIPGHYEAGMRGEIQ
                     IRSSGQH"
     gene            complement(12010..>12865)
                     /locus_tag="PK242_06310"
     CDS             complement(12010..>12865)
                     /locus_tag="PK242_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ93666.1"
                     /translation="TTASGRGTPADIDRIQLRASKGGVDTMVPLSSLVQVREGVAPRE
                     LNHFGQRRSATLTANLAPDYSLGQALTFLDQAAARVLKSGYTTDLNGISREFRSSQGA
                     LVVVFVLALLFIFLVLAAQFESFVDPLVIMFSVPLSMIGALLALKWSGGSLNVYSQIG
                     LITLVGLVTKHGILIVEFTNQLRQRGWDMLDALVHASSQRLRPILMTTGAMVLGAVPL
                     ALASGAGSESRRQIGWVIVGGMSLGTLLTLFVVPTVYALLARRAVPGANTTPVEAPQA
                     AQPAPTGH"
BASE COUNT         1971 a         4359 c         4423 g         2112 t
ORIGIN      
        1 tgctcgatca cctcgtcgga gccgcgcgtc tggatggtca tgcgcgacag gctggggtct
       61 tcggtgggcg ccacgatgag cgactcgatg ttgtagcccc gggccgaaaa caggcccacc
      121 acacgggaca gggcgcccgc ttcgttctcg atcagtacgg caatgatgtg tttcatgggt
      181 cgattcctct tttcgccgcc ctcccccgcg cacggtaatg cgcgggcttg gcggacaata
      241 gattcgtctt gctattaata cgatagctgc tggcgctgat ccagtctgcg ccagagccgt
      301 ttttcttaca actcttccga gcccagcagc atttcggtga tgcccttgcc gggctccacc
      361 atcgggaaca cgttctcggt cggatcggtg cggaagtcca tgaacaccgt gcggtccttg
      421 agcttgcgcg cctcgcgcag cgcgggctcg acgtcctgcg gccgctcgat cagcatgccg
      481 acgtggccat aggcttcggc cagcttgacg aagttcggca gcgcgtccat gtagctgtgg
      541 ctgtagcggc cggcgtactg caattcctgc cactgacgca ccatgcccag gtagcggttg
      601 ttgagcgaga tgatcttgat cggcgtgttg tactgcaggc aggtggacag ctcctggatg
      661 cacatctgca ccgagccttc gcccgtcacg caatacacct cgctctcggg cttggccagc
      721 ttgatgccca tggcgtaggg aatgcccacg cccatggtgc ccaggccgcc cgagttgatc
      781 cagcggcgcg gctcgtcgaa gcggtagtac tgcgccgccc acatctggtg ctggccgacg
      841 tcggaggtga tgtaggcgtc cgcgccgcgg gtcatttccc acagggtttc gatcaccttc
      901 tgcggcttga tgacgtcggt gttggcgcgg tcgtacttca tgcagtcgcg cgcgcgccag
      961 ccctcgaccg tcttccacca gtcggccagc gcggcctcat cggggcgcgc accggcctcg
     1021 cggatcatgc cgatcatctc ggtcagcacg tccttgacgt cgccgacgat gggcacgtcg
     1081 accttgacgc gcttggagat cgacgacggg tcgatgtcga tgtggatgat cttgcgctcg
     1141 ttctgcgcga aatgcttggg gttgccgatc acgcggtcgt cgaagcgcgc gcccacggcc
     1201 agcagcacgt cgcagtgctg catggcgttg ttggcttccc aggtgccgtg catgcccagc
     1261 atgcccaaaa agcgccggtc cgacgccggg aacgcgccca gccccatcag cgtgtgggtc
     1321 accgggcaat tgagcagatc gaccagcgtg cgcagctcgg cgcaggcgtc ggccagcagc
     1381 acgccgccgc cggtgtagat gtagggccgc ctggccgcca gcagcagttg cagcgccttg
     1441 cggatctgac cgccgtggcc cttcctgacc gggttgtacg agcgcatctc aacgctctgc
     1501 ggatagccct tgtaggccgt cttctcgaac gacacgtcct tggggatgtc caccaccacc
     1561 gggccgggcc ggccggagcg cgcgatgtga aaggccttct tcatcacctc ggccatgtcg
     1621 cgcacgtcct tgaccaggaa gttgtgcttg acgatggggc gcgtgatgcc cacggtgtcg
     1681 cattcctgga aggcgtcctg gccgatggcg gccgtcggca cctggccggt gatgatcacc
     1741 atcgggatcg agtccatgta ggcggtggcg atgccggtga cggcattggt cacgccgggg
     1801 ccggaggtga ccagggccac gccgacgtcg ccggtggcac gcgcgtagcc gtcggccgcg
     1861 tgcaccgccg cctgctcgtg acgcaccagc acatgctgga tggtgtcctg cttgtagaag
     1921 gcgtcgtaga tgtgcagcac ggcgccgccg gggtaacccc agacgtattt gacgccttcg
     1981 gcctgcaggg ccttgatgag gatttcggcg ccgcgcagct cctgcggggc ctgggaagtg
     2041 tcggcggacg ccgcgacggc ggccgacttc agttcggctt tgctgatttc catgtcgttc
     2101 aacctttcga gaatttctct gacgaaaaac cttgggtgcc ccttgccagc ccttgtgagg
     2161 ccgcttcagg acttgaggcc agacaccatg ggcggcctca cttctgccgc cggcgctgac
     2221 ccgtttgctc tgatgactgc aacacgcatt atcgcactgc aacatggcat gctgcgccga
     2281 ctcccaaaaa tcgtgttcgc ccccggtgcc ataatctcgc gcgcactgcc cttgcccgcc
     2341 ccttgcacct cgcggcgagg cgcccgttca ccgtctcgcc cgccgcacat ttccttggcc
     2401 accgatcagg aactctccga ctttctgcgc aacgtcgaga agcgggcgtt caagcgcacg
     2461 gtctaccacg tgcgcgacga cgacgcggcg ctggacatcg tgcaggacag catgatgaag
     2521 ctggccgagc actacggcga caagccggcc gccgagctgc cgctgctgtt ccagcgcatc
     2581 ctgtccaaca gcacgctgga ctggtttcgt cgccagaaga cccgtaacgc gctgttttcc
     2641 aacatgagcg acttcgacgc cgaagatggc gacggagaat tcgacctgct ggagatcttc
     2701 gccggcgcca gcgacagcac gcagatcgaa agcgccgaag acgccacccg gcgcgcgcag
     2761 gtgctgcacg aaatcgagtt acaaatccag gccctgccca accgtcaacg cgaggcattt
     2821 ctgttgcgtt actgggagga aatggatgtt gccgaaacgg cagcggtcat ggggtgttcc
     2881 gaaggcagtg tcaagacgca ctgctcgcgc gcagtccagg cgctggcgaa ggccttgaga
     2941 gccaagggta tccagccatg aacacgaatt tcagtcaaca ccacacggaa tcgctgcgca
     3001 gccgcttcgg cctgcgcgtg ggcgcgcgcc tgtcggccgg cacccatgag ctgccgcacg
     3061 acgtgcgcga acgtctgcgg gcggcgcgcg agcgcgcgct ggcccagcgc cgccagccgg
     3121 tgacacagcg ggtcgcgcgc cccagctggg cagcggtgcg tcagggcgca accctgacgc
     3181 tcggcgcggg cgacaacggc gccggcttct ggggccgcct ggcgtccgcc gccttgatgt
     3241 tggtgctggt ggccggcctg gtcgccatcg atgtggtgca ggacaacgac cgcgccaccg
     3301 aggtggccga gctcgacgcc gccctgctga ccgacgatct gccgccggag gcttacgccg
     3361 atccgggttt cctccaattc atcaagtccg atctcggccg cacctcttcc accctcagcc
     3421 agtgattcag acccatgggc cgctggcatt gcccctgatg cgccatttcc tgccggttct
     3481 gttgcttgcc ctgacggcgt tgacgctgtg gccggcgcat gcccaggagg tggccaagcc
     3541 gggctcagcg gccgccgtcg gtggccagat cgagtggcgc agcctgagca cggcccagcg
     3601 caccgcgctg gcgccgctgg agcgtgactg ggcgtcgctg ggttccgacc agcagcgcaa
     3661 gtggatcgcc ctgacccaga acttcagccg catgacgccg caggagcgcg ccaccctgca
     3721 gggccgcatg accgaatggg ccaacctcac accggcccag cgcacccagg ctcggctcaa
     3781 tttcggcgag gtgcgcaagg tgcccaccga cgaacggcgc gccaaatggg aggaatacca
     3841 ggcgctgccc gaagaagaac gcgcccgcct ggcgcgcgag cggcccaagc ctccggcagg
     3901 cgcggcgccg gcgctgcggc ccgtgccggc cgaacggctg gtgcgcccgg cgcgcccgcc
     3961 ggtgcccgtg gtgctgcctg aagcccccgc cagtgcgccg gtgccggccc gggtcaaccg
     4021 caatacactg ctgccccagc gccccaggcc ggccgcgccc aacaccgaat cgcgctgagc
     4081 ggccggtggc tttgctccgg ggcgtgtcgg acccgggcgc ccgcgccccg cttgttgcat
     4141 gaactcctcc gccgccaccc catccacgcc cattgccgtc gcaggctcgc cgctgcgcgc
     4201 cccctcgctg cgccgccgca tggcctgctg gctgtacgaa ggcacgctga tgttcggcgt
     4261 ggtggtgctg ccgggcctgg tgttcggtat tgccaccaac acgcgccatg cgctggacaa
     4321 ccgccacgcg ctgcaggcgc tgctgttcgt ggtgctggcc ttgtatttcg tgtacttttg
     4381 ggtgaagggc cagaccctgc ccatgaaaac ctggcacatc cgcgtggtcg accggcacgg
     4441 cctgccgctg cgctggccgc gcgcgctgct gcgctacctg ctggcctggc tgtggtttct
     4501 gccgccgctg gcggccatgg cgccgtttcg cctgtcgggc agtgaggtca ccgtgctgat
     4561 gttcggctgg atcgccgtct gggccctgct gagccggttt cagcgcgaac agcagttctg
     4621 gcacgacgcc tgggccggca cgcgcctgat cgacgtgcag cccgatgccg ccaggcgtcg
     4681 ccagtgatcc gtgccgccgc gctccccgac gccatgccgc ccaccgatcg ccctcgcgag
     4741 caagcgcccg cccatgcgca ccagcggcgc accgggctgg cgcggctgtg gcacgccaca
     4801 ggccattcgc tggccgggct ccgggccggc tggggcgaac ccgccttccg ccaggaagcc
     4861 ctgctggccc tggtgctgct gccggcggcg ctgtggcttg gccgcacctg ggtcgaggtg
     4921 gcgctgctgg tcggatcgct gctgctggtg ctgatggtcg agctgctcaa ctccggcatc
     4981 gagtcggcca tcgaccgcat cggccccgaa tggcatgctt tgtccaaacg cgccaaggac
     5041 atgggttcgg ccgccgtgct gctgagcctg ctgctggggg cgggtatctg gctcgccgcg
     5101 ctgtacaccc acctgttctc ctcatgaccg ctgctgctgc ttctgaacct gctttttcca
     5161 tctgtgtcta ctgtggctcg cgcccgggcg aggcgcaggc gtttgccgac gtcgccgccc
     5221 aggtcggcca ctggatcggt cgccacggcg gccagctggt gtacggcggc ggccgcgccg
     5281 gcctgatggg cgtggtggcc gacgccacgc tggcggcggg cgggcgcgtg gtcggcatca
     5341 tcccgcgcgt gctggtggag cgcgagcacg cgcaccgcgg ctgccacgag ctgcatgtgg
     5401 tggaaaccat gcacgagcgc aagcgccaga tggccgaacg ggccgacgcc tttctggcgc
     5461 tgccgggcgg catcggcacc ttcgaagagt tcttcgagac ctggacctgg cgccagctcg
     5521 gctaccacga caagccggtg ggcctgctca accaggacgg ctactacgac gccatgctga
     5581 gctttctgca gcacagcgtg gccagcggtt tcatgggggc ggcgcagatg gcgctgatcg
     5641 aggtcggcac cgactggcgg gccctgcttg gagccctggt ccacgccacc ggctttccgg
     5701 cacccgacct gctcagccag atctgacggc gcgccgccag gctcagcctt cgggcagaaa
     5761 cagctgctgc agatcgctca gaaagtcgaa accgcgcgcc gtcggccgga tccaggcccc
     5821 ctcgcgctgc agccagccgg actgctcggc ctgctgcagc gcgctggcga tgctgctggg
     5881 cggcatgccg gtgcgctcgg tgtagtcggt caggctgaag ccgtccttca ggcgcagcgc
     5941 gttcagcatg aattccagcg gcagctcggc acgcgccact tcgtgcgcct gcgccacggc
     6001 gtcgccggcc agcgcctgct ccatgtagcg ctcgggctgg cggtgccgca cctgacgcac
     6061 gatgcggtgc ggaaagctca gcttgccgtg cgcaccggcg cccagcccca ggtagtcgcc
     6121 gaattcccag tagttgaggt tgtgcacgca gcggtggccg gggcgcgcgt aggccgacac
     6181 ttcgtagcgc gccaggccgg cgtcggcggt cagcgcggtg atgcggtcga gcatgtccca
     6241 ggccgtgtcc tcgtccggca ggcgcggcgg gaacttggcg aacacggtgt tgggctcgat
     6301 ggtcaggtga tagatcgaca ggtgcggcgg cgcaaagtcg agcgccgtgc gcaggtcctg
     6361 ctccagctcg gccagggtct ggccgggcag cgcatacatc aggtcgaggt tgaaggtgtc
     6421 gaaagcgcgc gccgcttcct gcagcgcggc gcgcgcctgg gcgccgtcgt gcacccggcc
     6481 cagcgccttc aggtgccggt catgaaagct ctgcaccccg accgacaggc gcgtcacgcc
     6541 ggccgcccga aacgccgcga aacgctcgcg ctcgaaggtg ccggggttgg cttccagcgt
     6601 gatctcgcac atcggctcca gcggcaggcg cgcgcgcacg ccggcgatca gacggtcgat
     6661 gtcgtccggc tcgaacaggc tgggcgtgcc gccgccgatg aacacgctgt agaccggccg
     6721 cccccacacc agcggcaggc tggcctccag gtcggccagc agcgcgtcga cgtagcgccc
     6781 ctgctgggcc cgcgcgtcga caccggtgcg ccgctcgtgc gagttgaagt cgcagtacgg
     6841 gcacttcttc aggcaccagg gcaggtgcag ataaagcgac agcggcggca gcgccggcag
     6901 ctgcagggtg ccggggcgca ggtagtgccg gacgtcgttg ttcacagcca gcgctcgcgc
     6961 atcaaggcca ccatcgccgc cacggcgcga ccacggtggc tgagggcgtt cttcgtgtcg
     7021 gtcggcatct cggcaaaggt ctggccgtgc tcggggatga acatcaccgg atcgaagccg
     7081 aagcccccgc tgccgcgcgg cgcgcgcgcg atctcgccca ccacgcgtcc gacggcgatc
     7141 agcggctcgg ggtcgtcggc gcggcgcagc gccaccaggg tgctgaccat ggcggcgcgg
     7201 cgattgtcga tgccctgcat ctgctccagc aacgcgcaca cgttgccggc gtcgcctttg
     7261 gcgtagccga actgggtgcc gtagtacgcc gtgtccacgc ccggcaggcc gccgaaggcg
     7321 tcgacgcaca ggccggcgtc gtcggccagc gccggcagac cgctggccgc ggcggcgtgg
     7381 cgcgccttgg cgagcgcgtt ttccacgaag gtgcggaagg gctcggccgc ctcgccgatg
     7441 cccagttcag cctggcgcac caggtccatg cccagcgggg ccagcatggc ttgcagctcg
     7501 gccagcttgc ccgggttgtt ggaggccagc acgattctca ttcaaatcac cctctggcgc
     7561 ttgtccagca agcgccacaa gctatcaatt caatagcgaa cggcgttgca tctcgatcag
     7621 ctcgccaatg cccttttcgg ccagccgcag gagctggtcc atctcggcgc gcgaaaacgc
     7681 cgcgccctcg gccgtgccct gcacctcgac gtagtggccg gcgccggtca tgaccacgtt
     7741 catgtcggtg tcgcaggatg agtcctgggc gtagtcgaga tcgagcatcg gcacgccgcc
     7801 gacgatgcct accgagatcg ccgccaccgg gtgcaggatc ggcgactcgg cgatcagccc
     7861 gcgcgccagc aggccgctca ccgcgtcctg cgccgccacg aacgcgccgg tgatggccgc
     7921 ggtgcgcgtg ccgccgtcgg cctgcaacac gtcgcagtcc agcaggatgc tgcgctcgcc
     7981 cagcttggtg aggtcgaaca cggcgcgcag gctgcgcccg atcagacgct ggatctcctg
     8041 cgtgcgcccg ctttgcttgc cgcgcgccgc ttcgcggtcg ccccgcgtgt gggtggcgcg
     8101 cggcagcatg ccgtattcgg ccgtgaccca gccctcaccg ctgccgcgct tgtgcggcgg
     8161 caccttttct tccaccgagg cggtgcacag cacctgggtg tgaccaaatt cgatcagcac
     8221 cgaaccttcg gcgtagcggg tgtagtggcg ggtgatgcgc accgggcgca actggtcggg
     8281 cgcacggtcg gtgcgcgcgg gggacgaaac ggaggcattc atggtgtcga agaaagtcgg
     8341 ccaggcgcgc agcatgccac gccgcggccc gcacccgctc agcggcggtt catggccgtg
     8401 cgctggatgg cggcattgat ctcggcgatg gagcgctcga tggcctcgtc gtccaggtcg
     8461 tccagcagcg gatcgtaggc cccggcctgg atggtggagg cgaacacctc gtcgctcagc
     8521 acctgggttt cggtgaagcc gctgtgctcg aacgccgatt cgggcgggct ttcccactcg
     8581 aacgccagca ccgtgacgtt gtccgagtgt tcgcccgcgt tctgcaaggc cacatcaacc
     8641 aacgccggca cggccttggc caccggcagc gcgcccaggg tgcgcacgat caggtcatcg
     8701 tccaggctgc cccacagacc atcggagcac agcagcacgc ggtcgccctg catcaggcgc
     8761 agcgggccag cgatgtcgaa catcggccgt acggtcgagc ccaggcaggt gaacagcacg
     8821 ttgcggttga tggcttcgga tgtcatgccg gcaccggcct gcgcctcgct gtacgaatgg
     8881 tcgcgggtgc gcaccagcaa ctggccgtcg cgcaccacgt acagtcgcga gtcgccgcag
     8941 tgcatccagt gcagctcccc ttcctgcacc agggcagcca ccagggtggt acgcggggta
     9001 tccagcagac cgcgctcgct ggcatagcga atgatctggt ggtgcgcgcc catcagactg
     9061 gacgccagaa agctggtcgg caccgcgagg ctcggccgcg cctcgcgctg aaacagcgcc
     9121 gacatcgtct gcaaggcaat ctgggccgcc acttcaccct cggggtgtcc gcccatgccg
     9181 tcggccagtg caaacaaccc cgcatcgcgc gtgtagctgt aacccatgcg atcctcgttt
     9241 ttctcacgcc cgccgcggcg gctgagctgg aagaccgaaa atttcatggc agaagaactt
     9301 acttcggacg cgcaccgaag cgcgtgaccc gcttcagact ctgcttggtg tcgttcatca
     9361 tgttgtcgaa ctgcaggcgc acgcgctcgc cgaccgacag gcgggtgtac tggcgctcac
     9421 cttcgcgcga cagttccttc tgcagcgcaa ataccgattg cgggcgcgac agcgggtcga
     9481 gcgacatgca ccattcgacc acctcgatca ggttgtccga gtagacaccg cgcatgcgcg
     9541 tcagcgacag cgacagacgg tccttttcaa gtcgctgcgg cgcgtcgttg ggcggatagc
     9601 cgtgcatgca cgaatagatg caggcaccga tggcgtacac gtcggtccac ggtcccatgg
     9661 tggagtcgcg ccggtacatc tcgggtgcgg caaagcctgg cgtgtacatg ggacgagtga
     9721 agttgccttc cttgttcagc acctcgcgcg cagcgccaaa gtcgatcatc accgccttgt
     9781 tgtcgtcggt gatgaagatg ttggccggct tcagatccag gtgcagcatc ttgtgctgat
     9841 gaacgatgcg cagaccgcgc aggatctcgt cgaacagcga ccggatggtg gactcgcgga
     9901 acaccttctg cttcttcaac tcgcgcgcag tgacgatgaa atcctgcagg gtcgcgccct
     9961 ccaggtaatt catcaccatg tagacggtct cgttctggcg gaagaagttc agcacgctca
    10021 ccaccgacgg gtgggagatc tgcgccagcg accggccttc ctcgaaaaag ctcttgagac
    10081 ccagccggta cagggacagc ttttcgggcg ccacttgggg caccagttcg cccggttcac
    10141 gcaatgtcaa tgccgagggc aggtattcct tgatggccac ctgctggcct tcacggtcga
    10201 ccgccaggta caccacaccg aagccgccgg ccgagatcct gcgcagcacg cggtagccgc
    10261 cgatctcggt atcgggcggc aggggcgcgg gcttggcctt tgacataatt tgcggcgtcg
    10321 atcggcccgg gccactgggt cgcgcaccga tcccgctggg acccgtcgcc aagaccaccc
    10381 tgtacccgga aaagtaatat gtcagtttac agtatgaccg gttacgccag cggtcagcaa
    10441 gccctcgccg gcgacgccga gggcggctcc ggcgccaaga atgcacgggg cggcgtaggc
    10501 atggagttgc gctccgtcaa cagccgcttt ctggacctga gctttcggct ggccgacgaa
    10561 ctgcgtccgc acgaaccggc gctgcgcgag ctgctgggcc agcgcctgcg gcgcggcaag
    10621 gtggagttgc gcgcctggct gcagggccag gccgatgacg cgctggccga gccgtcgcca
    10681 cgcctgctgc agcgactggc cacgctggag gaccaggtgt gcaactggct gccacaggcc
    10741 acgcgcctgt cggtggccga ggtgctgcgc ctggcgggcg gcaccgacag cctgagcgac
    10801 gaggccgcgg ccgaggcgct gcgggcgctg gcgcgccggg tgctggacga cttcgttgcc
    10861 gcacgcgagc gcgaaggcgc acggctggcg cgcctgatcg gcgaacgcgt cacccaactg
    10921 cgcgcgctgg cacgcgccgc cgaaccgctg gtgccgcagg cggtgcaggc gcagaagcag
    10981 cgctttctgg accgctggcg cgaagcgctg gccagcggcg agggtcagat cggcgccgag
    11041 gcggcgcagg agcgcgccct ggccgaggcc acggcctacg ccatccgcat cgacgtggcc
    11101 gaggaactgg gccggctggc ggcacacctg gacgaaatcg aagcggtgct gcaccagggc
    11161 ggcgaaaccg gcaagcggct cgacttcctg atccaggagt tgcaccgcga agccaacacg
    11221 ctgggttcga aggcggccgt gctggaactc acgcgcatca gcgtcgacat gaaggtgctg
    11281 atcgagcaga tgcgcgagca ggtgcagaac atcgagtgaa ccgacgatgc gacatcggcc
    11341 agtggcgaac ccgttgaacc aggggtgacg aggggacgga ggtggatgcc gcggcgcttc
    11401 gcgggctgtg atcgagcgca gacttcaaag cgccgccctc ccatccaggc gcactggggc
    11461 ggtccttgcg ctcagtgttg accgctgctg cggatctgga tttcacccct catgccggct
    11521 tcgtagtggc cggggatgag gcaggcgaag tgcacggtgc cggcgcggct gaagtgccag
    11581 acgatttccc cctgctggcc gggggccagc gtgatctggt tcggttcggc gtgcgtcatg
    11641 ccgggctggc gtgccatctg gtcgcggtgc gccagcaaat cctgcagggt gcccaggctg
    11701 agttcgtgcg ccacctggcc ggtgttgcgc accaccaggc gcacggtttc gccctggcgc
    11761 acggcaaggc gttcggggct gaagcgcatg cggtcgttca tgtcgatgcg cacgctgcgg
    11821 ctggcgcgcg cggccacgcc gggctggccg atggcgctgt cggtggcggc gtgctcgccg
    11881 tgcgggccat ggtggctgcc ggcagcgaag gcggcgccgc cacacagggt cagggcggcc
    11941 agcagggcgc ggcggggggt gctcgggcgg gtcatggggt gcctctcggg atcaggcgaa
    12001 acaaggtgtt cagtggcccg tgggcgccgg ctgggcggcc tgtggcgctt cgaccggggt
    12061 ggtgttggcg ccgggcacgg cgcggcgcgc cagcagggcg tagacggtgg gcacgacgaa
    12121 cagggtgagc agggtgccca ggctcatgcc gccgacgatg acccagccga tctggcgccg
    12181 gctttcggag ccggcaccgc tggccagcgc cagtggcacg gcgcccagca ccatggcgcc
    12241 ggtggtcatg aggatggggc gcaggcgctg gctgctggcg tgcaccaggg cgtcgagcat
    12301 gtcccagccg cgctggcgca gctggttggt gaattcgacg atcaggatgc cgtgcttggt
    12361 gaccaggccg accagggtga tgaggccgat ctgcgaatac acgttgagcg aaccgccgct
    12421 ccatttgagc gccagcagcg cgccgatcat ggacagcggc accgagaaca tgatgaccag
    12481 cggatcgacg aagctttcga actgcgccgc cagcaccagg aagatgaaca gcaacgccag
    12541 cacgaacacc accaccagcg cgccctggct ggagcggaat tcacgcgaga tgccgttcag
    12601 gtcggtggtg tagccgctct tgagcacgcg cgccgcggcc tggtcgagga acgtcagcgc
    12661 ctggccgagc gagtaatcgg gcgccagatt ggcggtgagc gtggccgagc ggcgctggcc
    12721 gaagtggttg agctcgcgcg gcgccacgcc ttcgcgcacc tgcaccaggc tggacagcgg
    12781 caccatggtg tccaccccgc ccttgctggc gcgcagctgg atgcggtcga tgtcggccgg
    12841 ggtgccgcgg ccgctggcgg tggtc
//
LOCUS       DAOOOG010000061        12816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_5389_length_12816_cov_32.109082, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000061 DAOOOG010000000
VERSION     DAOOOG010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12816
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5389_length_12816_cov_32.109082"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1421)
                     /locus_tag="PK242_06315"
     CDS             complement(135..1421)
                     /locus_tag="PK242_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOZ93667.1"
                     /translation="MSLFNWQEKPASQLAAGGVVHIDERLPWPQTVAMGVQHVIAMFG
                     ATVLAPILMGFDPNLAIFMSGVGTLLFYFITGGRVPSYLGSSFAFIGVVIAATGYAGQ
                     GPNPNIAVALGAIIVCGLLYALIGALVNAVGTRWIERLMPPVVTGAVVAVIGLNLAAI
                     PVKNMAASNFDSWMQLVTFVSVALVAVFARGMVQRLLILVGLAIASVIYVLLANGLGL
                     GKPMDFSGIASAAWVGLPRFTAPVFEGNAMLLIAPVALILVAENLGHLKAVAAMTGRN
                     LDPYIGRAFTADGVATMISGAAGGTGVTTYAENIGVMAATRIYSTAVFVVAALMALVL
                     GFSPKFGALIQAIPLPVMGGVSIVVFGLITIAGARIWVENKVDFGDPANLLTAAITLI
                     LGTGDFTLKFGSFALGGIGTATFGAVLLHALLRRRA"
     gene            complement(1578..2339)
                     /locus_tag="PK242_06320"
     CDS             complement(1578..2339)
                     /locus_tag="PK242_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOZ93668.1"
                     /translation="MTRPAYTTEPLFQQIAQTLRQRLRDGSYPVGIPLPGERELTREF
                     GVARSTVRAALARLQEEGCITRLPGQGTLPVAEPSTGEHSKIRNGLLENILRFGQRTR
                     TTLVSTQYMAAPDAVAQALQLPAGSRVMRVVRVRKSHRTPLLCTEAYLPAAVAEGIAP
                     QMLHDMPLLEAIEQAGHAFAQGEQELMAVVAPAEVAELLRIAPGAALLRVNRVVADAQ
                     GRPLQYLIGHYAPDHYHYRMRLSRTGGSTTVWITH"
     gene            complement(2397..3251)
                     /locus_tag="PK242_06325"
     CDS             complement(2397..3251)
                     /locus_tag="PK242_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HOZ93669.1"
                     /translation="MSLKQRLAAPGITIAPGVYDALTALLVQQAGFPAAYLSGASIAY
                     TRLGRSDIGLLGLDDVAQVTSCIRERVELPLIVDADTGFGNALNVMRSVRQLERAGAS
                     AIQLEDQTSPKRCGHLQGKSVIATREMVGKLRAAVDARRDDDTLIIARTDAVAVEGFD
                     AALERAARYAEAGADLLFVEAVRSRDQMQQLNQVLGALCPLMANMVEGGMTPVTSAQE
                     LAEIGYRLVIFPGGTVRALTHALQGYLASLHTHGSTLPWRDRMLDFDALNAVIGTPEL
                     LALGQRYD"
     gene            complement(3248..3814)
                     /locus_tag="PK242_06330"
     CDS             complement(3248..3814)
                     /locus_tag="PK242_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HOZ93670.1"
                     /translation="MNHSTSPAPVNAQPRPGRCWVLGDDINTDYLAPGKYMKFGIEDI
                     ARHCLEDVLPHFAAEVRAGDLIIAGRNFGTGSSREQAVEVLRHLGVQCVIARSFAGLF
                     YRNGFNLGLPLLLGPADMPAPAWGIAPGTPAVLDLAQARLQLPDARQLSCQPIPTHLL
                     ALVADGGLVPHLRRRIERQRPQHTRTAP"
     gene            complement(3811..5085)
                     /locus_tag="PK242_06335"
     CDS             complement(3811..5085)
                     /locus_tag="PK242_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOZ93671.1"
                     /translation="MPATLAQKLVARACGQTQVQPGQIVTCRVDLAMMHDSGGPRRLK
                     PHLDALGVGVWDPERIVVVTDHFVPASDAGSRQIVQLARDWVREQGIRHFYDEQGICH
                     VVTPRHGHLRPGLFAVGGDSHSCTGGAFGAYMFGIGATEMLGVVVTGEIWLRVPHTIA
                     MDWHGRLGPGVCAKDMMLHLCTRFGMDGGQYEAIEFCGEAVRALSMNERMTVSNMTAE
                     LGGQVGLIAPDATTAAWLRGVGVPEAALQDLEQWQTDAAAPLLAHHRLDAAALAPQVS
                     LPGSPAQAQEAGTLAGERLDIAYIGACTGAKLDDLRMAAQILRGRRTAAGVRLLVAPA
                     SAEDQRQAAAEGVLQVLQQAGAELLPNACGACAGYGAHTFAPDTTVISTTARNFTGRM
                     GAASSRVFLGSPYTVAASAVTGHITDPRELLA"
     gene            complement(5088..6056)
                     /locus_tag="PK242_06340"
     CDS             complement(5088..6056)
                     /locus_tag="PK242_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ93672.1"
                     /translation="MFNRRSWLWAGAAVALGAWMAPASAQDSRPITIVVGASPGGSTD
                     TLARTIAQSMGTTLKRTVIVDNKPGAGGNIAAQHVARSAPDGSTLLMSFTSHTINATL
                     FKKLPFNPVTDFTPITMVAQVPSVLVVRKSAPFDSVQGLIDYARKNPGKLSFAIGGQG
                     SSLHLAGEQFKQMTQTDIVNIPYKGTGPALTDLLADNTVDLMFASTINVLPHYKQGKI
                     KFLGVSSPQPLAQFAGLPAIAETVPGYQSQAWFGLFGPAKLPQAVTEALYGAVRVALD
                     SPEYRQRMQSEAATVPTMTPAQFADFIKKDVDFWGAIVKKSGAQVE"
     gene            complement(6365..7453)
                     /locus_tag="PK242_06345"
     CDS             complement(6365..7453)
                     /locus_tag="PK242_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOZ93673.1"
                     /translation="MSAADPTRPTAPEWADPARQAAFERWLARLQPVHRLQPDSLRVA
                     SADASFRRYFRLDTEDGASRIVMDAPPAHENCAPFVRVAALMQAAGLHVPQILDWDEA
                     GGFMLMTDLGTRTLMEVLDRDRPEANRPRFAQAIDALLAWQQASRPGELPPYDEALLR
                     RELQLFPDWYLGRHRGVTLDAEQTATLERQFALIVARNLAAPQVHVHRDFMPRNLMVG
                     APEGPLGVLDFQDAVHGPITYDIASLMRDAFLSWDEEFVLDITVRYWERARKLGLLDF
                     EDWHGDFGSFWRAVEWMGLQRHLKVAGIFARLTLRDGKPRYLADAPRFIAYIRHTASR
                     YRELAPLLRLLDQVEGRTAVDGWAFGRV"
     gene            7580..9967
                     /gene="lptD"
                     /locus_tag="PK242_06350"
     CDS             7580..9967
                     /gene="lptD"
                     /locus_tag="PK242_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOZ93674.1"
                     /translation="MPVSPPFLPIAAASRVRPRWALHPLLAGVLALLCGAATAQPGEP
                     LALRATPLLAEQPPSHVGAATTVYGQRVRGRIGLETVLEGQAELRQPGTVAQAERLSY
                     DQGSDIVTVSGGVRLNKAGDRYTAESGQLRVDAMEGFLLRPTYRFLASGAHGEAERLE
                     WLDRDRASVIQGSYTTCPRGGPDWVPDWIVRADRLDLDREDDEGRASGAVLEFKGVPV
                     LPVPALSFPISERRRSGWLPPTLGLDNKSGLDLSVPYYWNIAPHRDATFTPGLMSRRG
                     VDLAGEFRYLENDYQGRINANLTPNDRLRDGRNRWGYFLRHNGQHDSGIDGIGRVGLG
                     LNLNRVSDNDYWRDFSRRGLSLTTRLLANDGALSWSRGDLSLHARALKWQTLQDVAAP
                     IVPPYDRLPQLTARWGRSNDRGFDYAIEGDFTRFRADPFWTGQPNAERGFVQAQLARP
                     FTRPWGFFTPRVQLHASHYRFDGPISGGATSASRVLPTVSLDGGLVFERQAGFFGRDL
                     TQTLEPRLKYVYTPYRDQHRLPLYDTGAYDFNFATIWAENAFAGNDRIVDNNLVTGGL
                     TTRLLDPQTGAEVLRLGVAQRYRFSGQQVTLPGGLPTARGLSDLMLGASVNWDPRWAF
                     DSVVQYNLDTRRSTRTTVQARYSPEKFHTVSAAYRHQRDLRSESLDIGWQWPLADLAW
                     RQGEPGGRSAGGGSSCQGKWYGVGRLNYSLQDRRLVEGILGFEYDAGCWIGRVVLEKL
                     NSSIHSSTKRIMFQVELIGLSRLGSNPLRALRNQIPRYQYLREETSSPSRFTDYE"
     gene            9960..11483
                     /locus_tag="PK242_06355"
     CDS             9960..11483
                     /locus_tag="PK242_06355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOZ93675.1"
                     /translation="MSNSLPRRPSSWALLPLLLALAWPASGQAPSTAPAPAPSKASGK
                     KLRPAKRPAPTRAAPAPAPAPAPQDPAPAPAAAPASAPASAAPAARSASSTPREADYI
                     VALVNSEPVTNHEVRARLARAQRQLAERGAPVPPADALRQEVLERLIAERAQLQYARE
                     TGLKVDAAALTQAELSIARQNQLTSVAELHRRVEQEGIPVKDFQDDVRNQVLLARLRE
                     REIEPRLRVTDAEVEAFIREQTGVNPAAVDLNLAMILVAVPEGSSAAELARLQARADE
                     VARRARAGEDFAALALEFSDANNRGRDGGVLGLRPSERYPDLFVRSVQRARVGDIVGP
                     VKSEAGWHVLKLLERKQNKELPEVRIPQTHVQHILLRTGPAQSEQVARQRLADYKRRI
                     QAGQASFEQLAREHSQDASATDGGDLGWAPPGQFVPEFEQAMNNLDPGQISDPVTTRF
                     GVHLIRVEGRREQVLGAAEQRQLARNVLREKKAEEAFETWAREVRGRAYVELREPPR"
     gene            11602..12372
                     /gene="rsmA"
                     /locus_tag="PK242_06360"
     CDS             11602..12372
                     /gene="rsmA"
                     /locus_tag="PK242_06360"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOZ93676.1"
                     /translation="MKHIPRKRFGQHFLADPAIIDAIVRAIDPRPGQAMVEIGPGLAA
                     LTQPLVERLGRLTVIELDRDLAARLRAHPQLEVIESDVLKVDFGQIQQARAATKIRVC
                     GNLPYNISSPILFHLLPFAALIEDQHFMLQKEVIDRMVAAPATADYGRLSVMLQWRYA
                     MENVLFVPPSAFDPPPKVDSAVVRMVPRSEFAPVDADLLSELVRVAFSQRRKLLRHTL
                     GRWLSERGFAGAFDVQRRAEEVPVAEYVALAHELAAPR"
     gene            complement(12398..>12816)
                     /locus_tag="PK242_06365"
     CDS             complement(12398..>12816)
                     /locus_tag="PK242_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ93677.1"
                     /translation="PLRFDALRRWLDAIEFVQRDERGNWPLEQFDAQIAQNKRDFDTR
                     FAKAQALLAEGQWTASMDELLEIILRDRKWNDEAARKTYVAILELLTPPPAKPGAGDG
                     GKSASGIELTGKAAQQQDPQAELLSSYRRKLSMALN"
BASE COUNT         1894 a         4450 c         4571 g         1901 t
ORIGIN      
        1 tgtcgtcctg cgcccatgcc gggccggtca gcgtcggtgc cagcagggcc gacagcgtca
       61 gcacctggcg acgggtgggc atctcggtca tcgtggttct ccttgttggc ggtggcttgc
      121 gccgaggcga tggctcaggc gcgccggcgc agcagcgcgt gcagcagcac cgcgccaaag
      181 gtggcggtgc cgatgccgcc gagggcgaag ctgccgaact tcagcgtgaa gtcgcccgtg
      241 cccaggatca gggtgatggc ggcggtcagc aggttggccg ggtcgccgaa gtcgaccttg
      301 ttttccaccc agatgcgcgc accggcgatg gtgatcagac cgaacaccac gatgctgacg
      361 ccgcccatca ccggcagcgg gatggcctgg atcagcgcgc cgaacttggg cgagaagccc
      421 agcaccagcg ccatcagcgc cgccaccacg aacaccgcgg tggaatagat gcgcgtggcc
      481 gccatgacgc cgatgttttc ggcataggtg gtgacgccag tgccgccggc ggcaccgctg
      541 atcatggtgg ccacaccgtc ggcggtgaag gcgcggccga tgtaggggtc gaggttgcgc
      601 ccggtcatgg ccgccaccgc cttcaggtgg cccaggttct cggccaccag gatcagcgcc
      661 accggggcga tcagcagcat ggcgttgcct tcgaagaccg gcgcggtgaa gcgcggcagc
      721 cccacccagg cggcgctggc gatgccggaa aaatccatcg gcttgcccag ccccagccca
      781 ttggccagca gcacgtagat cacgctggcg atcgccagcc cgaccaggat cagcaggcgc
      841 tgcaccatgc cgcgcgcaaa caccgccacc agcgccacgc tgacgaaggt gaccagctgc
      901 atccagctgt cgaagttgct ggccgccatg ttcttgaccg ggatggccgc caggttcaga
      961 ccgatcaccg ccaccaccgc gccggtgacc accggcggca tcaggcgctc gatccagcgc
     1021 gtgccgacgg cgttgaccag cgcgccgatc agcgcgtaca gcaggccgca gacgatgatg
     1081 gcgcccagcg ccaccgcgat gttggggttg ggcccctggc cggcgtagcc ggtggcggcg
     1141 atcaccacgc cgatgaaggc aaacgacgaa cccaggtagc tgggcacccg gccgccggtg
     1201 atgaagtaga acagcagcgt gccgacgccg ctcatgaaga tcgccaggtt ggggtcgaac
     1261 cccatcagga tcggcgccag cacggtggct ccgaacatgg caatcacgtg ctgcaccccc
     1321 atggcgaccg tctgcggcca gggcaggcgc tcgtcgatgt gcaccacacc gcctgccgcc
     1381 agttgagacg cgggtttttc ctgccagttg aacaggctca tgggctccac tcctttgctt
     1441 tggggttgtc agggggcgcg cgccggcgcg accgcccgcg atggtagtgc agcgcgggcc
     1501 cggctgccgg cggcgcctgc ggcgcatcgg gcggcgggcg gcgggcggcg ggcggcgggc
     1561 ggcgggccgc tgcgccgtca gtgcgtgatc cacaccgtgg tcgatccccc ggtgcgcgac
     1621 aggcgcatgc ggtagtggta gtggtcgggt gcgtaatggc ctatcaggta ttgcagtggc
     1681 cggccctggg catcggccac cacgcggttg acgcgcagca gcgcggcgcc gggggcgatg
     1741 cgcagcagtt cggccacctc cgccggggcg accacggcca tcagctcctg ctcaccctgg
     1801 gcaaaggcgt ggccggcctg ttcgatggct tcgagcagcg gcatgtcgtg cagcatctgc
     1861 ggggcgatgc cttcggccac cgcggccggc aggtaggcct cggtgcacag cagcggtgtg
     1921 cggtgtgact tgcgcacgcg caccacgcgc atcacgcggc tgccggccgg cagctgcagg
     1981 gcctgggcca cggcatcggg cgctgccatg tattgcgtcg acaccagcgt ggtgcgcgtg
     2041 cgctggccga agcgcaggat gttctccagc aggccgttgc ggatcttcga atgctcgccg
     2101 gtgctgggct cggccaccgg cagcgtgccc tggccgggca ggcgggtgat gcagccttcc
     2161 tcctgcaggc gcgcaagggc cgcgcgcacg gtgctgcgcg ccacgccgaa ttcgcgcgtc
     2221 agttcgcgtt cgcccggcag cggtatgccc acggggtaac tgccgtcgcg caggcgctgg
     2281 cgcaaggtct gggcgatctg ctgaaacagg ggctcggtcg tgtatgcggg gcgcgtcaag
     2341 acggtctcct ggccgggcac gccatgcggc cggtcaggtg attatcggcg tcggggtcag
     2401 tcgtagcgct ggcccagcgc cagcagctcg ggcgtgccga tgacggcgtt gagcgcatcg
     2461 aagtccagca tgcgatcgcg ccagggcagg gtgctgccgt gggtgtgcag gctggccaga
     2521 tagccttgca gcgcgtgcgt gagggcgcgc accgtgccgc ccgggaagat caccaggcgg
     2581 tagccgatct cggcgagctc ctgcgcgctg gtcacgggtg tcatgccgcc ttcgaccatg
     2641 ttggccatca gcgggcacag tgcgcccagg acctggttga gctgctgcat ctggtcgcgg
     2701 ctgcgcacgg cctcgacgaa cagcaggtcg gcgccggcct cggcgtagcg ggcagcgcgc
     2761 tccagcgcgg cgtcaaagcc ctccaccgcc acggcgtcgg tgcgcgcgat gatcaacgtg
     2821 tcgtcgtcgc ggcgcgcgtc cacggcggcg cgcagcttgc cgaccatctc gcgagtggca
     2881 atcacggact tgccttgcag gtggccgcag cgcttggggc tggtctggtc ttcgagctgg
     2941 atggccgagg cgccggcgcg ctcgagctgg cgcacgctgc gcatcacgtt gagcgcgttg
     3001 ccaaagccgg tgtcggcatc gacgatgagt ggcagctcca cgcgctcgcg gatgcagctg
     3061 gtgacctggg ctacgtcgtc caggcccagc agaccgatgt cggagcggcc cagacgcgtg
     3121 taggcgatgc tggcgcccga caagtacgcg gcgggaaagc ccgcctgctg caccagcagg
     3181 gcggtcaggg cgtcgtacac gcccggggcg atggtgatgc cgggcgcggc caggcgttgc
     3241 ttcaggctca tggtgcggtg cgcgtgtgtt gcgggcgctg gcgctcgatg cgccggcgca
     3301 ggtgtggcac caggccgcca tcggccacca gtgccagcag gtgggtgggg atgggctggc
     3361 aggacagctg ccgtgcatcg ggcagttgca gccgcgcctg cgccagatcc agcaccgccg
     3421 gcgtgcccgg cgcaatgccc caggcgggcg ccggcatgtc ggccgggccg agcagcagcg
     3481 gcagacccag gttgaagccg ttgcggtaga acagcccggc aaacgagcgt gcgatcacgc
     3541 attgcacgcc caggtggcgc agcacttcga cggcctgctc gcgcgaggag ccggtgccga
     3601 agttgcgccc ggcgatgatc aggtcgccgg cgcgcacctc ggcggcaaag tgcggcagca
     3661 cgtcctccag gcaatgccgg gcgatgtcct cgatgccgaa tttcatgtac ttgcccggcg
     3721 ccaggtagtc ggtgttgatg tcgtcaccca gcacccagca gcgcccgggc cggggctggg
     3781 cgttgaccgg tgcgggtgag gtggagtggt tcatgccagc agctcccgtg gatcggtgat
     3841 atggccggtg acggccgagg ccgccaccgt atagggcgag cccaggaata cgcgcgagga
     3901 tgcagcaccc atgcgccccg tgaagttgcg cgccgtcgtg ctgatgacgg tggtgtcggg
     3961 cgcaaaggtg tgggcgccgt agccggcgca ggcgccgcag gcgttgggca gcagctcggc
     4021 gccggcctgc tgcagcacct gcaacacgcc ttcggcggcg gcctggcgct gatcttcggc
     4081 gctggcgggt gccaccagaa ggcgcacgcc ggcggcggtg cggcgcccgc gcaggatctg
     4141 ggcggccatg cgcaggtcat cgagcttggc acccgtgcag gcgccgatgt aggcgatgtc
     4201 caggcgctcg ccggccagcg tgccggcctc ctgtgcctgg gccggcgagc cgggcaggga
     4261 cacctggggc gccagcgccg ccgcgtccag gcggtggtgc gccagcagcg gcgccgccgc
     4321 gtcggtctgc cactgttcca ggtcttgcag cgcggcctcg ggcacgccca cgccgcgcag
     4381 ccatgcggcc gtggtggcgt cgggcgcaat caggccgacc tgaccgccga gctcggccgt
     4441 catgttcgac accgtcatgc gctcgttcat ggacagggcg cgcacggcct cgccgcagaa
     4501 ttcgatcgcc tcgtactgtc cgccatccat gccgaagcgc gtgcacaggt gcagcatcat
     4561 gtccttggcg cacacgccgg gccccaggcg cccatgccag tccatggcaa tggtgtgcgg
     4621 cacgcgcagc cagatctcgc cggtcaccac cacgcccagc atctcggtgg caccaatgcc
     4681 gaacatgtag gcaccgaacg cgccgccggt gcaggaatgg ctgtcgccgc caacggcaaa
     4741 cagccccggg cgcagatggc cgtggcgcgg cgtgaccacg tggcagatgc cctgctcgtc
     4801 gtagaagtgg cgtatgccct gctcgcgcac ccagtcgcgc gccagctgca cgatctggcg
     4861 gctgccggcg tcgctggcgg gcacgaaatg gtcggtgacg acgacgatgc gctccggatc
     4921 ccagacgccc acgcccagcg cgtccagatg gggcttcagg cgtcgcgggc cgcccgagtc
     4981 gtgcatcatg gccagatcga cgcggcaggt gacgatctgg ccgggctgca cctgtgtctg
     5041 gccgcaggcg cgcgccacca gtttctgggc cagggtagcg ggcatggtca ctccacctgc
     5101 gcgccggact tcttcacgat ggcgccccag aagtccacgt ccttcttgat gaagtcggca
     5161 aactgcgccg gcgtcatggt cggtacggtg gccgcctcgg actgcatgcg ctggcggtat
     5221 tcgggcgaat ccagcgccac gcgcacggcg ccatacagcg cttcggtcac ggcctgtggc
     5281 agcttggccg ggccaaacag gccgaaccag gcctgcgact ggtagcccgg caccgtctcc
     5341 gcaatcgccg gcaggccggc aaactgcgcc agcggctgcg ggctgctgac acccaggaac
     5401 ttgatcttgc cctgcttgta gtgcggcagt acgttgatgg tgctggcaaa catcagatcc
     5461 acggtgttgt cggccagcag gtcggtgagc gccgggcccg tgcccttgta ggggatgttc
     5521 acgatgtcgg tctgcgtcat ctgcttgaac tgctcgccgg ccaggtgcag cgacgagccc
     5581 tggccaccga tggcaaagct gagcttgccg gggttcttgc gcgcatagtc gatcaggccc
     5641 tgcacactgt cgaacggtgc gctcttgcgc accacgagca cgctgggcac ctgggccacc
     5701 atggtgatgg gggtgaagtc cgtcaccggg ttgaagggca gtttcttgaa cagcgtggca
     5761 ttgatggtgt ggctggtgaa gctcatcagc agcgtggagc catcgggcgc gctgcgcgcc
     5821 acatgctgcg cggcgatgtt gccgccggcg ccaggcttgt tgtccacgat gacggtgcgt
     5881 ttgagcgtgg tgcccatgct ttgggcgatg gtgcgcgcca gggtgtcggt ggagccgccc
     5941 ggcgaggcgc cgacgacgat ggtgatgggg cggctgtcct gcgcgctggc cggcgccatc
     6001 cacgcaccca gcgcgacggc tgcgccagcc catagccacg atctcctgtt gaacatgtcg
     6061 gtctccttgt gcaccagagg ttgaaggggg agcttccgat gctgggcacg cgtggtctgc
     6121 gcttcaatag gaccagctgg ttcgctgggg tttgcaccgt ggcgggcttc atgaagggat
     6181 gtctgcggcg ccgatggccc cgcatggcac ccgactggca gccgactggc agccgaggag
     6241 catgccgacc cgccccgcgc gctatgtaaa caatagctgt ttgcgatgac ccggcgggcg
     6301 ttggggctcg atttgtttgg attgttgcgg ccggcttcga gcgccggcat cgccgcttgg
     6361 ggcgtcagac ccgcccaaac gcccagccat cgacggcggt gcgcccctcg acctggtcga
     6421 gcagccgcag cagcggcgcc agttcgcggt agcgcgatgc ggtgtggcgg atgtaggcga
     6481 tgaagcgtgg cgcgtccgcc aggtacctgg gcttgccgtc gcgcagggtc aggcgggcga
     6541 agatgccggc cactttcagg tggcgctgca ggcccatcca ttcgaccgcg cgccagaagc
     6601 tgccgaagtc gccgtgccag tcctcgaagt ccagcagccc cagcttgcgc gcgcgctccc
     6661 agtagcgcac cgtgatgtcg agcacgaact cctcgtccca gctcaggaag gcgtcgcgca
     6721 tcaggctggc gatgtcgtag gtgatggggc cgtgcaccgc gtcctggaag tccagcacac
     6781 ccagcgggcc ttcgggcgcg cccaccatca ggttgcgcgg catgaagtcg cggtgcacat
     6841 ggacctgcgg cgcggccagg ttgcgcgcca cgatcagcgc gaattggcgt tccagggtgg
     6901 cggtctgttc agcgtccagc gtcacgccgc ggtgccggcc cagataccag tcgggaaaca
     6961 gctgcagctc acgccgcagc agcgcctcgt cgtagggcgg cagctcgccg ggccggctgg
     7021 cctgctgcca ggccagcagc gcgtcgatgg cttgcgcgaa gcgggggcgg ttggcctcgg
     7081 gccggtcgcg gtcgagcacc tccatcaggg tgcgcgtgcc caggtcggtc atgagcatga
     7141 agccgccggc ctcgtcccag tccaggatct gcggcacatg caggccggcg gcctgcatca
     7201 gcgcggccac gcggacgaag ggcgcgcagt tctcgtgcgc gggcggcgcg tccatgacga
     7261 tgcggctggc gccgtcttcg gtgtccaggc ggaagtaacg ccggaagctg gcgtcggccg
     7321 aggccacgcg caggctgtcg ggttgcagcc ggtgcaccgg ctgcaggcgc gccagccagc
     7381 gctcgaatgc ggcctggcgg gcggggtcgg cccattcggg ggcggtgggg cgggtcggat
     7441 cggcggcgct catggataat cgattctagg aggctgtcgg acttggagcg ccgaagccga
     7501 gcgccagcgc cagccgcctc cgaaaatgcg cccatccggc tcacggcttg gcgggcgcgt
     7561 cgatccgacg gttgttgccg tgcctgtttc acctccattc ctgcccattg ccgccgcctc
     7621 gcgtgtccga ccgcggtggg cgctgcaccc gctgctggcg ggcgtgctgg cgctgctgtg
     7681 cggtgcggcg acggcgcagc ctggcgagcc gctggcgctg cgcgccacgc cgctgctggc
     7741 ggagcagccg ccctcccatg tcggcgcggc gaccaccgtg tacggccagc gcgtgcgtgg
     7801 ccgcatcggt ctggagacgg tgctggaagg ccaggccgag ttgcgccagc ccggcaccgt
     7861 ggcgcaggcc gaacggctca gctacgacca gggcagcgac atcgtcaccg tcagcggcgg
     7921 cgtgcggctg aacaaggcgg gcgaccgcta caccgccgaa tccggccagc tgcgcgtgga
     7981 cgccatggaa ggctttctgc tgcggcccac ctaccgcttt ctggccagcg gcgcgcacgg
     8041 cgaggccgag cgcctggaat ggctggaccg cgatcgcgcc agcgtcattc agggcagcta
     8101 caccacctgc ccgcgcgggg ggccggactg ggtgcccgac tggatcgtgc gcgccgaccg
     8161 cctggacctc gaccgcgagg acgacgaagg gcgcgccagc ggcgccgtgc tggaattcaa
     8221 gggcgtgccg gtgctgccgg tgccggcgct gagctttccg atcagcgagc ggcgtcgctc
     8281 cggctggctg ccgcccacgc tggggctgga caacaagagc gggctggatc tgtccgtgcc
     8341 gtactactgg aacatcgcac cccaccgcga tgccaccttc acgcccggcc tgatgtcgcg
     8401 ccgcggggtc gatctggcgg gcgagttccg gtatctggaa aacgactacc agggccgcat
     8461 caacgccaac ctgacgccca acgaccgcct gcgtgacggc cgcaaccgct ggggctactt
     8521 cctgcgccac aacggccagc acgacagcgg catcgacggc atcggccgcg tcgggctggg
     8581 gctcaacctc aaccgcgtca gcgacaacga ctactggcgc gacttctcgc gccgcggcct
     8641 gtcgctgacc acccgcctgc tggccaacga cggcgcgctg tcgtggtcgc gcggcgatct
     8701 gtcgctccac gcccgggcgc tcaagtggca gaccctgcag gacgtggccg cccccatcgt
     8761 gccgccctac gaccgcctgc cgcaactgac ggcgcgctgg ggacgcagca acgaccgcgg
     8821 cttcgactac gcgatcgagg gcgacttcac gcgctttcgc gccgacccgt tctggaccgg
     8881 ccagcccaac gccgagcgcg gcttcgtgca ggcgcagctg gcgcggccct tcacccggcc
     8941 ctggggcttt ttcacgcccc gggtgcagct gcacgccagc cactaccggt tcgacgggcc
     9001 gatcagcggc ggcgccacct cggccagccg cgtgctgccc accgtcagcc tcgacggcgg
     9061 cctggtgttc gagcgccagg ccggcttttt cggccgcgac ctcacgcaga cgctggagcc
     9121 gcgcctgaag tacgtctaca cgccctaccg cgaccagcac cggctgccgc tgtacgacac
     9181 cggcgcctac gacttcaact tcgccaccat ctgggctgaa aacgcctttg ccggcaacga
     9241 ccgcatcgtc gacaacaacc tggtcaccgg cggcctgacc acgcgcctgc tcgacccgca
     9301 gaccggcgcc gaggtgctgc gcctgggcgt ggcccagcgc taccgctttt caggccagca
     9361 ggtcaccctg ccgggcggcc tgccaaccgc gcgcgggctg tccgacctga tgctgggcgc
     9421 ctccgtcaac tgggacccgc gctgggcctt cgacagcgtg gtgcagtaca acctggacac
     9481 gcgccgctcc acccgcacca cggtgcaggc gcgctactcg ccggaaaagt tccacaccgt
     9541 cagcgccgcc taccgccacc agcgcgatct gcgcagcgag tcgctcgaca tcggctggca
     9601 gtggccgctg gccgacctgg cctggcgcca gggcgagccg ggcgggcgca gtgccggcgg
     9661 cggcagcagc tgccagggca agtggtacgg cgtcgggcgg ctgaactaca gcctgcagga
     9721 ccggcgcctg gtcgaaggca tcctgggctt cgaatacgat gccggctgct ggatcggccg
     9781 cgtggtgctg gaaaagctca acagctcgat ccactcctcc accaaacgca tcatgttcca
     9841 ggtcgagctg atcggcctgt cgcgcctggg cagcaacccg ctgcgtgcgc tgcgcaacca
     9901 gattccgcgc taccagtacc tgcgcgaaga aacctcctca cccagccgct tcaccgacta
     9961 tgagtaacag cctgccccgc cgcccgtcct cctgggcgtt gttgccgctg ctgctggcgc
    10021 tggcctggcc ggccagtggt caggccccct cgaccgcccc ggcgcccgcc ccgtccaagg
    10081 ccagcggcaa gaagctgcgt ccggccaagc gcccggcgcc gacccgcgcc gcgccggcgc
    10141 cggccccggc cccggccccg caggatccgg cgcccgctcc cgcggccgcg ccagccagcg
    10201 cccccgccag cgccgcgccc gcggcgcgca gcgccagcag cacgccgcgc gaggccgatt
    10261 acatcgtcgc cctggtcaac agcgagccgg tcaccaacca cgaggtgcgc gcccgtctgg
    10321 cacgtgccca gcgccagctc gccgagcgcg gtgccccggt gccgccggcc gatgcgctgc
    10381 gccaggaggt gctcgagcgc ctgatcgccg agcgcgcgca gctgcagtac gcgcgcgaaa
    10441 ccgggttgaa ggtcgacgcc gcggcgctga cccaggccga actgtcgatt gcgcgccaga
    10501 accagctgac cagcgtggcc gagctgcacc ggcgcgtcga gcaggaaggc attccggtga
    10561 aggacttcca ggacgacgtg cgcaaccagg tgctgctggc gcgcctgcgc gagcgcgaga
    10621 tcgagcccag gctgcgcgtc accgacgccg aggtggaagc cttcatccgc gagcagaccg
    10681 gcgtcaaccc ggccgccgtc gacctgaacc tggccatgat cctggtggcc gtgcccgaag
    10741 gcagcagcgc cgccgagctg gcgcggctgc aggcgcgcgc cgacgaagtg gcgcgccgcg
    10801 cccgcgccgg cgaggacttc gccgcgctgg cgctggagtt ttccgacgcc aacaaccgcg
    10861 gccgcgacgg cggggtgctg ggcctgcgcc cgtcggagcg ctaccccgat ctgttcgtgc
    10921 gcagcgtgca gcgcgcgcgc gtgggcgaca tcgtcgggcc ggtcaagtcc gaagccggct
    10981 ggcacgtcct caagctgctg gagcgcaagc agaacaagga gctgcccgaa gtgcgcattc
    11041 cgcagaccca cgtgcagcac atcctgctgc gcaccgggcc ggcgcagagc gagcaggtgg
    11101 cgcgccagcg cctggccgac tacaagcgcc gcatccaggc cggacaggcc agcttcgagc
    11161 agctggcgcg cgaacattcg caggacgcca gcgccaccga cggcggcgat ctgggctggg
    11221 cgccgccggg gcagttcgtg ccggagttcg agcaggccat gaacaacctc gacccgggcc
    11281 agatcagcga cccggtcacc acccgcttcg gcgtgcacct gatccgcgtc gaaggccggc
    11341 gcgaacaggt gctgggtgct gccgagcaac gccagctggc gcgcaacgtg ctgcgcgaga
    11401 agaaggccga ggaagcgttc gagacctggg ccagggaagt gcgcgggcgc gcctacgtcg
    11461 agctgcgcga gccgccgcga tagggcccga gccggcgttc gacgtgtgaa gaagaggttg
    11521 agcgttcggc gttgagcgtt cagcgtgaat accggagctt tcgcggccac gtccatctgt
    11581 caacgctcaa cgctcaacgg catgaagcac atcccccgca aacgcttcgg ccagcacttt
    11641 ctggccgacc cggcgatcat cgacgccatc gttcgcgcca tcgacccgcg cccgggtcag
    11701 gccatggtcg agatcggccc cggcctggcg gcgctgacgc agccgctggt cgagcgcctg
    11761 ggacgcctga cggtgatcga gctggaccgt gatctggcgg cgcggctgcg cgctcacccg
    11821 cagctcgagg tcatcgagtc tgatgtactg aaagtcgatt tcggtcagat acaacaagcg
    11881 cgagcagcta caaaaatcag agtgtgtggc aacctgccct acaacatctc cagcccgatc
    11941 ctgtttcacc tgctgccctt tgccgcgctg atcgaggacc agcatttcat gctgcaaaag
    12001 gaagtgatcg accgcatggt ggccgcgccc gccacggccg actacggccg cttgtcggtg
    12061 atgctgcagt ggcgctacgc gatggagaac gtgctgttcg tgccgcccag cgcgttcgat
    12121 ccgccgccca aggtggacag cgcggtggtg cgcatggtgc cgcgcagcga gttcgccccc
    12181 gtcgatgccg acctactctc cgagttggtc cgcgtcgcct tcagccaacg ccgcaaactg
    12241 ctgcgccaca cgctgggccg ctggctgagc gagcgcggct ttgccggcgc gttcgacgtg
    12301 cagcggcgcg ccgaggaagt gccggtggcc gaatacgtgg cgctggcgca tgagctcgct
    12361 gcgccccgat gacttcgcgg cgttcaaggc catcgcgtca gttcagcgcc atgctcagct
    12421 tgcgccggta gctggacagc agctcggcct gcgggtcctg ctgctgcgcg gccttgccgg
    12481 tgagctcgat gccgctggcg ctcttgccgc catcgccggc gccgggcttg gcgggcggcg
    12541 gggtcagcag ctccaggatg gcgacgtagg tcttgcgcgc ggcctcgtcg ttccacttcc
    12601 tgtcgcgcag gatgatttcc agcagctcgt ccatgctggc cgtccactgg ccctcggcca
    12661 gcagcgcctg ggccttggca aagcgggtgt cgaagtcgcg cttgttctgg gcgatctggg
    12721 cgtcgaactg ctcgagcggc caattgccgc gctcgtcgcg ctgcacgaac tcgatcgcgt
    12781 ccagccagcg gcgcagggcg tcgaagcgca gcgggc
//
LOCUS       DAOOOG010000062        12751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_5430_length_12751_cov_26.084830, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000062 DAOOOG010000000
VERSION     DAOOOG010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12751
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5430_length_12751_cov_26.084830"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PK242_06370"
     CDS             <1..697
                     /locus_tag="PK242_06370"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428468.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOZ93678.1"
                     /translation="SKSYDNTIALFGPREQLRRQIMSIVTDSRAPGEPKDTEGSALYQ
                     IYRAFADDAEAAQFARAFADGIAWGEAKQRLFERIDAEIGPMRERYQHLMSHPAEVEA
                     LLRRGADKARALATPLMRELRQAVGLRALDTQVAAPRGRDSARPGLPSFKQYREQDGR
                     FYFKLTGAGGQVLLQSRGFESPREAGQAVKRLQSEGEHALGALAEWLEPVADALAPTL
                     TEALQSLQTAQQS"
     gene            803..1408
                     /locus_tag="PK242_06375"
     CDS             803..1408
                     /locus_tag="PK242_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ93679.1"
                     /translation="MAIYVIDDHPLMREAVVMLLRRIRPGSEVVELSQLSDLERAVET
                     RGAPELFCLDLKLPDTVGISGVKLLKRLYPDVPLAVISATPAADVEEWCTEAGADVYI
                     EKSAGASEIGAALRAMLQGDSEFADLEYGDSQTETKLSKRQKQLIVMLDEGLSNRDIA
                     ERLGISEHTVKVHLWRLFRRLGVKSRTQTLHFARTHGLLSS"
     gene            complement(1350..2765)
                     /locus_tag="PK242_06380"
     CDS             complement(1350..2765)
                     /locus_tag="PK242_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOZ93680.1"
                     /translation="MIPIPAPAPDPASRADWTEARQLAILMDGWPGTQLAVLALWALL
                     GGLLLLHAPGLRTGLWLGAALLVMAPGWRGWRSYRQRLAWAPLAEQRLRLRRQRASWL
                     VQALVWGMAPLCFAQRVAPEVEAMCWALTLGTGVLLVSWSSAHRRLAQGLASVLVAAL
                     LASLWLRLSGSPLRADLRGLWWQALLLLVYAAILWRIIGSQHQRVAEGIELSLRNARL
                     IESLREQTRVAQEAARFRTRFLAGAAHDLKQPVNAVGLYADWLSNEPELVDELAPKIL
                     QSTLAIHSLFDSLFDWVRLDAGHYELREREVAIRPLLSDLEVQFGPEARLKGLRLRVR
                     PADVVLRTDPVMLRRVIGNLLANAIRYTARGGVLLAARARPDGVSLEVWDTGIGIAQS
                     ELEQIFGEFYKVPTSGTEDGFGLGLSIVQALAERLGYRVTVRSRPGRGTVFRVWVPAP
                     AGLRNSAGRACERSAGSGSGS"
     gene            complement(2830..3894)
                     /gene="aroC"
                     /locus_tag="PK242_06385"
     CDS             complement(2830..3894)
                     /gene="aroC"
                     /locus_tag="PK242_06385"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOZ93681.1"
                     /translation="MSGNTLGTLFAVTNFGESHGPAIGCVIDGCPPGLALSEADIQPE
                     LDRRRPGSSKFVTQRQEPDQVEILSGVYQGHTTGTPICLLIRNQDQRSKDYGDIAQSF
                     RPGHADYTYWKKYGLRDPRGGGRSSARLTAPTVAAGAVARKWLREQCGTSFAGCMTHL
                     GELAIGFEDWAHVPRNPFFAPVADVSALEDHMNALRKAGDSCGARLRVVARGVPVGLG
                     QPIYDRLDADIAKAMMGLNAVKGVEIGDGFASVAHRGSQHGDSPLPGGGFASNHDGGV
                     LGGISNGQDIEVAVAIKPTSSILVPRASVNLADEPVEVITKGRHDPCVGIRATPILEA
                     LLALVVMDHALRQRAQCGRL"
     gene            complement(4028..4495)
                     /locus_tag="PK242_06390"
     CDS             complement(4028..4495)
                     /locus_tag="PK242_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOZ93682.1"
                     /translation="MRVRDILRLKGNALYTITPEDSLADAAQAMADRDIGSLVVMSHE
                     RVVGMLTFREVIQAAVKNGGSFAGLSVYRSMDPGPLICTLETELDEVRRMMLEHHTRY
                     LPVIDNRQLMGVISFYDVAKAVVDAQDFENQMLKAYIRDWPEEDAAPAATERA"
     gene            4605..5915
                     /locus_tag="PK242_06395"
     CDS             4605..5915
                     /locus_tag="PK242_06395"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HOZ93683.1"
                     /translation="MPGYADPGFDTLAVHAGARPDPATGARAVPIHLSTSFVFDSSDH
                     AAQLFNMERAGHVYSRISNPTNAVLEQRVAALEGGIGAIATASGQAALHLAIVTLMGA
                     GGHIVASSALYGGSQNLLHYTLRRFGIDTTFVRPGDPDGWRAAIRPNTRLLLGETLGN
                     PGLEVLDIPAVAQIAHAAGVPLLVDSTFTTPYLLKPLEHGADLVYHSATKFLSGHGTV
                     IGGVLVDGGSFDWDRSGRFPELTTPYEGFHGMVFAEESTVGAFLLRARREGLRDFGAA
                     LSPHSAWLILQGIETLPLRMERHIANTEQVVRFLAEHPFVARVGHPLLASHPSHALAE
                     RLLRHGARGAGSVFSFDLKGSRAQGRVFIDTLKLFSHLANVGDCRSLVIHPASTTHFR
                     MNDEALAAAGIGPGTIRLSIGLEDPQDLIDDLKRALKAAEKAGA"
     gene            5920..6729
                     /locus_tag="PK242_06400"
     CDS             5920..6729
                     /locus_tag="PK242_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ93684.1"
                     /translation="MFIDLHGAPLYAYTGGRPFDPAQPTVVFIHGVLNDHSVWILQSR
                     ALAHHGWNVLAIDLPGHGRSGGQPPATVEQAADDVLALLRAAGIARAALVGHSFGSLI
                     ALEAAARAPSQVSHLVLVGTAFPMAVSPALLEASVSAPEKAIHMVNVFSHSTLCPPPS
                     ALGPGTWLYGSSRALMRRVLASNPTVNLFHTGFQACDRYRGGEQAMAAVQCPTLLLVG
                     RHDQMTPPRAARALAAHARQATVVEVEAGHALMTEAPDAVLDALRQFLSRT"
     gene            complement(6834..7229)
                     /locus_tag="PK242_06405"
     CDS             complement(6834..7229)
                     /locus_tag="PK242_06405"
                     /inference="COORDINATES: protein motif:HMM:NF022592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HOZ93685.1"
                     /translation="MSPARLPSSPRLPLSRGVSITLQPGQARTLHLPAAGTLRVAQGA
                     LWVTRRGRHVDHPAQDLYVSAGLALALDAGDSVVIEPMHLAATPAAQVPPASFSWQAD
                     EGHCAGLLRWLRQHLQAAWRPAPARAPCA"
     gene            7349..8299
                     /locus_tag="PK242_06410"
     CDS             7349..8299
                     /locus_tag="PK242_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOZ93686.1"
                     /translation="MLHAPTHLRSRPIQVGHLRAFEAVARHLNFRLAAQELALSQPAV
                     SRQIQALEDDVGVPLFRRHTRAVELTEAGNQLLTAVGQALPRLDATVRHIRQSAGRRH
                     VSVTTFASFASMWLIPRLEAFQRDHPDIDIRVEASDTAVDLEVTDIDVAVRYAPAERM
                     PAPAQRLFGESVTPMASPWLLRSGPPLKRPEDLLRHTLIEAGDAYTPHLQWLTWRRWL
                     DANGLKDAQPKRWLSFNYAYQMVQAALSGQGVVLARPPLVAENLARGDLVEVFAAGAY
                     PQARLISPMAYWVLVSPHSRQRAEVRAFADWLLTQAQSTR"
     gene            complement(8512..9423)
                     /gene="rapZ"
                     /locus_tag="PK242_06415"
     CDS             complement(8512..9423)
                     /gene="rapZ"
                     /locus_tag="PK242_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOZ93687.1"
                     /translation="MTPFAPAPSAAAAPPPTGTELVVITGMSGSGKSIALRALEDAGY
                     YCVDNLPPELLRPFIALEDQRRARRLAIAMDVRSAESLPQLPPLLDELAARGVHLRQI
                     FLEANDHTLVRRFSETRRRHPLSLADPGDVQQALLQAIARERAQMADLRERSLVIDTS
                     VLLPSQLLATVKELIAAPLAHLTLVFESFAFKRGITLDADYVFDVRMLPNPYYLPELR
                     PLTGRDQPVIDYLAQQPEVQRMQAQIAQFLRHWLPALARDHRSYVTVAIGCTGGQHRS
                     VYLVEQLAAELGSSWTTLRRHRELDGL"
     gene            complement(9420..11120)
                     /gene="recN"
                     /locus_tag="PK242_06420"
     CDS             complement(9420..11120)
                     /gene="recN"
                     /locus_tag="PK242_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOZ93688.1"
                     /translation="MALRHLSLRDFVIVAALDLALADGFTALTGETGAGKSILIDALQ
                     LVLGGRGDALWVREGQPRCDIAAEFELPDAARPWLAEHGFDTDADALLLRRTIDAAGK
                     SRAWINGSPATLAQLRTLGEWLVDIHGQHAWQSLTRPAAVRALLDAYAGIDTRPLTTA
                     WQAWRQADTALQAARSAQEQLAQERERLLWQIGELDKLAPGEGEWDELNQRHARLSHA
                     QALIDAAQAALAALEGDDGDGGALPALGHAHDALAARRDIEPEFATLAQQLDDAIAQA
                     RDAAHSLHGYLRRANLDPEQLLALDQRLGQWLGLARRFRRPPQELHTVLAQWRTELAQ
                     LDAAADLAQLQARADAAQQTYRQSADAVSRQRRQTAPQLAAAVTEAMQLLGMAGGRFE
                     VQLQALAEPAPHGLENVAFLVAAHAGSAPRAIERVASGGELSRLALAIAVTTSQLGTA
                     GTLVFDEVDAGVGGAVAATVGRLLQRLGRDRQVLCVTHLPQVAACADAHLQVSKQTGD
                     GLPTSQVAPLVGEARTREIARMLGGEQLSDTTLAHAREMLALPAQPPGAAARPRKVRA
                     "
     gene            complement(11125..12021)
                     /locus_tag="PK242_06425"
     CDS             complement(11125..12021)
                     /locus_tag="PK242_06425"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOZ93689.1"
                     /translation="MSLHFHRVALVGKYQDAATGSAPESTRQVIADIAGFLVEQGCEV
                     ALAERTAVSTGLRQYPALDVPQIGTDCDLAVVVGGDGTMLGIGRQLARHGTPLIGINQ
                     GRLGFITDIPLGDYRRVLPTMLTGGYEEDPRSLMHGQVMRDGQCVFEALAMNDVVVNR
                     GAVASMVELRVEVNGRFVANHRADGIIVATPTGSTAYALSAGGPLLHPMVPAWALVPI
                     APHTLSNRPIVLADSVEIAIEIVAGRDASASFDMQSITSLHHGDRIVVRRSDHRMRLL
                     HPRGWSYFDTLRGKLHWNTGGV"
     gene            12049..>12751
                     /gene="hrcA"
                     /locus_tag="PK242_06430"
     CDS             12049..>12751
                     /gene="hrcA"
                     /locus_tag="PK242_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOZ93690.1"
                     /translation="MLDDRARLLLKTLIERYIADGQPVGSRTLSRASGLELSPATIRN
                     VMSDLEELGLIASPHTSAGRVPTARGYRLFVDTMLTAQRAELEAPELPAAQPQKVIAQ
                     AAQLLSSMSQFVGVVMAPRRSSVFRHIEFLRLSERRLLLIIVAPDGDVQNRVLFTDTD
                     HTASELQQASNYLNAHFSGLDIEQVRLRLRHEVDSLRGEIAKLMHAAVQLGSEAMTQA
                     QDEVVISGERNLLAVS"
BASE COUNT         1824 a         4554 c         4533 g         1840 t
ORIGIN      
        1 gagcaagagc tacgacaaca ccatcgccct gttcggcccg cgcgagcagc tgcgccggca
       61 gatcatgagc atcgtcaccg attcgcgcgc gccgggcgaa cccaaggaca ccgagggctc
      121 ggcgctctac cagatctacc gcgcctttgc cgatgacgcc gaggcggcgc agtttgcgcg
      181 cgcctttgcc gacggcatcg cctggggcga ggccaaacag cgcctgttcg agcgcatcga
      241 cgccgagatc gggccgatgc gcgagcgcta ccagcacctg atgtcccatc cggccgaggt
      301 cgaggccctg ctgcggcgcg gcgccgacaa ggcgcgcgcg ctggccacgc cgttgatgcg
      361 cgagctgcgc caggccgtgg ggctgcgtgc gctcgacacc caggtggcgg cgccgcgcgg
      421 gcgcgactcg gccaggccgg gcctgcccag cttcaagcag taccgcgagc aggacgggcg
      481 cttttacttc aagctcaccg gcgccggtgg ccaggtgctg ctgcaaagcc ggggcttcga
      541 atcgccgcgc gaggccggcc aggccgtcaa gcgcctgcag agcgagggcg agcacgccct
      601 gggcgcgctg gccgaatggc tggaaccggt ggccgacgcg ctggcgccga ccctgaccga
      661 ggcgctgcag tcgttgcaga ccgcacaaca aagctgatcc gctgcgccgg gcgcacgggc
      721 ctggcgcccc gcctgctcgc atcggcgggc ggactggtgc acaatcgcaa cctatacaca
      781 aaaaggttta ggagcagcga tcgtggcgat ctatgtcatc gacgaccatc ccctcatgcg
      841 cgaggcggtg gtgatgctgc tgcggcgcat tcgaccgggt tcggaggtgg tcgagctgtc
      901 gcagctgtcg gatctggagc gtgcggtgga aacccgcggc gcgcccgagc tgttctgcct
      961 ggatctgaaa ctgcccgaca ccgtgggcat ttcgggcgtc aagctgctca agcgcctgta
     1021 ccccgacgtg ccgctggcgg tgatctccgc cacgcccgcc gccgatgtgg aggagtggtg
     1081 caccgaggcc ggtgccgacg tctacatcga gaagtcggcc ggcgccagcg aaatcggcgc
     1141 cgccctgcgc gccatgctgc agggtgacag cgagttcgcc gacctcgaat acggcgacag
     1201 ccagaccgaa accaaactct ccaagcgcca gaaacagctc atcgtgatgc tggacgaagg
     1261 gctgtccaac cgcgacatcg ccgagcgcct gggcatcagc gagcacaccg tcaaggtgca
     1321 tctgtggcgg ctgtttcgcc gcctgggcgt caagagccgg acccagaccc tgcacttcgc
     1381 tcgcacgcac ggcctgctga gttcctgagc cccgccggcg ccggcaccca gacgcggaac
     1441 acggtcccgc gcccgggccg cgagcgcacc gtgacgcggt agcccagccg ctcggccagc
     1501 gcctgcacga tcgacaggcc caggccgaag ccgtcttcgg tgccgctggt gggcaccttg
     1561 tagaactcgc cgaagatctg ttccagctcg ctctgggcga tgccgatgcc ggtgtcccac
     1621 acctccaggc tgaccccgtc ggggcgcgcg cgcgccgcca gcagcacgcc gccgcgcgcg
     1681 gtgtagcgga tggcgttggc cagcaggttg ccgatcacgc ggcgcagcat caccgggtcg
     1741 gtgcgcagca ccacgtcggc cgggcgcacg cgcagccgca gccccttgag ccgggcttcg
     1801 gggccgaact gcacctccag gtccgacagc aggggacgga tggcgacctc gcgctcgcgc
     1861 agctcgtaat ggccggcgtc cagccgcacc cagtcgaaca gcgagtcgaa caggctatgg
     1921 atggccaggg tcgattgcag gatcttgggc gccagctcat cgaccagctc gggttcgttg
     1981 ctcagccagt cggcgtacag gccgacggcg ttgaccggct gcttcaggtc gtgcgccgcc
     2041 cccgccagaa agcgggtgcg aaagcgtgcc gcctcctgcg ccacgcgggt ctgctcgcgc
     2101 agcgactcga tcaggcgcgc gttgcgcagg ctcagctcga tgccttcggc cacccgctgg
     2161 tgctggctgc cgatgatgcg ccacaggatg gcggcataca ccagcagcaa cagcgcctgc
     2221 caccacaggc cgcgcaggtc ggcgcgcagc ggcgagccgc tcaggcgcag ccacaggctg
     2281 gccagcaggg ccgccaccag cacgctggcc agaccctgtg ccagccgccg gtgcgccgac
     2341 gaccacgaca ccagcagcac ccccgtgccc agcgtcagcg cccagcacat ggcctccacc
     2401 tccggcgcca cgcgctgggc aaagcacagc ggcgccatgc cccagaccag cgcctgcacc
     2461 agccagctgg cgcgctggcg gcgcaggcgc agccgctgtt cggccagcgg cgcccaggcc
     2521 agccgctgcc ggtagctgcg ccagccgcgc cagcccggcg ccatcaccag cagggcggcg
     2581 cccagccaca ggccggtgcg caggccgggc gcgtgcagca gcagcagccc gcccagcagc
     2641 gcccacagcg ccagcaccgc cagctgggtg cccggccagc cgtccatcag gatggcgagc
     2701 tggcgtgcct cggtccagtc ggcgcggctg gccgggtccg gggcgggggc cgggatgggg
     2761 atcacgaggt gctattcatt caatagctgc ttgcgctttc cagtcaagcg ctggagcctt
     2821 gtttgatgcc taaagacggc cacactgggc gcgctggcgc agcgcgtggt ccatcaccac
     2881 cagcgccagc agcgcttcca gaatgggcgt ggcgcggatg ccgacgcagg ggtcgtggcg
     2941 gcccttggtg atgacttcca ccggctcgtc cgccaggttg accgaggcgc gcggcaccag
     3001 gatcgagctg gttggcttga tcgccaccgc gacctcgatg tcctgaccgt tgctgatgcc
     3061 gcccagcacg ccgccgtcgt ggttgctggc aaagccgccg ccgggcagcg gcgagtcgcc
     3121 gtgctggctg ccgcggtgcg ccacgctggc aaagccgtcg ccgatctcca cccccttgac
     3181 ggcgttcagc cccatcatgg ccttggcgat gtcggcgtcc agccggtcgt agatcggctg
     3241 acccagcccc accggcacgc cgcgcgccac cacgcgcagg cgcgcgccgc acgagtcgcc
     3301 cgccttgcgc agcgcgttca tgtggtcctc cagcgccgac acgtcggcca ccggggcgaa
     3361 gaacgggttg cgcggcacat gcgcccagtc ctcgaagccg atggccagct cgcccaggtg
     3421 cgtcatgcag ccggcaaagc tggtgccgca ctgctcgcgc agccacttgc gtgccaccgc
     3481 gccggcggcc acggtgggcg cggtcaggcg tgccgaggat cggccgccgc cgcgcgggtc
     3541 gcgcaggccg tacttcttcc agtaggtgta gtcggcgtga ccggggcgaa agctttgcgc
     3601 gatgtcgccg tagtccttgc tgcgctggtc ctggttgcgg atcagcaggc agatcggcgt
     3661 gccggtggtg tggccctggt agacgccgga caggatctcc acctggtcgg gctcctgccg
     3721 ctgggtgacg aacttgctgc tgccggggcg gcgccggtcc agctcgggct ggatgtcggc
     3781 ctccgacagc gccaggccgg gcgggcagcc gtcgatcacg cagccgatgg ccgggccatg
     3841 ggattcaccg aaattcgtga cggcaaacag agtgccgagg gtgttgccgc tcatggggcg
     3901 ccattatcgc cgctggcgtg gcgtcggccg ggccggccgc cagacccccg gattcatggt
     3961 gacaaaggcc agaaaccttg gtgcagcaag cgccggcagc tcctgaaaac gcagcaaccg
     4021 aggcgcctca ggcgcgctcg gtggcggccg gcgcggcgtc ttcctcgggc cagtcgcgga
     4081 tgtaggcctt gagcatctgg ttctcgaagt cctgcgcgtc caccaccgcc ttggccacgt
     4141 cgtagaagct gatcacgccc atcagctggc ggttgtcgat caccggcagg tagcgcgtgt
     4201 ggtgctccag catcatgcgg cgcacctcgt ccagctcggt ctccagggtg cagatcagcg
     4261 gccccggatc catgctgcgg tacaccgaca ggccggcgaa cgagccgccg ttcttcaccg
     4321 ccgcctggat cacctcgcgg aaggtcagca tgcccaccac gcgctcgtgc gacatcacca
     4381 ccagcgagcc gatgtcacgg tcggccatgg cctgcgcggc gtcggccagg ctgtcttcgg
     4441 gcgtgatggt gtagagggcg ttgcccttca ggcgcaggat gtcgcggact ctcatggcgt
     4501 ggctttcggt ggcgccggcg ggccggcgtc gcaggaatat agcccacaat acggcgcggc
     4561 ccggcccgcg cgccgcaccc cttgccccct gactaggaga ccccatgccc ggctacgccg
     4621 accccggttt cgacacgctc gcggtgcacg cgggcgcgcg gcccgacccc gccaccggcg
     4681 cgcgcgccgt gccgatccac ctgagcacct cgttcgtgtt cgactccagc gaccacgccg
     4741 cccagctctt caacatggag cgcgccggcc acgtctacag ccgcatcagc aaccccacca
     4801 acgcggtgct ggagcagcgg gtggcggcgc tggagggcgg catcggcgcc atcgccaccg
     4861 ccagcgggca ggccgcgctg cacctggcca tcgtcaccct gatgggcgcc ggcggccaca
     4921 tcgtcgccag ctcggcgctg tacggcggca gccagaacct gctgcactac acgctgcggc
     4981 gcttcggcat cgacaccacc ttcgtcaggc cgggtgatcc ggacggctgg cgcgccgcca
     5041 tccgcccgaa cacgcgcctg ttgctgggcg agacgctggg caacccgggg ctggaggtgc
     5101 tggacatccc cgccgtggcg cagatcgcgc acgcggccgg cgtgccgctg ctggtcgatt
     5161 cgaccttcac cacgccctac ctgctcaagc cgctggagca cggcgccgac ctggtctacc
     5221 actcggccac caagttcctg tccggccacg gcacggtgat cggcggcgtg ctggtcgatg
     5281 gcggcagctt cgactgggac cgctcgggcc gttttcctga gctgaccacg ccctacgagg
     5341 ggtttcacgg catggtgttt gcggaagaaa gcaccgtcgg cgcctttctg ctgcgcgcgc
     5401 ggcgcgaagg cctgcgcgac ttcggtgccg cgctgtcgcc gcacagcgcc tggctgattc
     5461 tgcagggcat cgaaaccctg ccgctgcgca tggagcggca catcgccaac accgagcagg
     5521 tggtgcgctt tctggccgag cacccgttcg tggcgcgcgt cggtcatccg ctgctcgcat
     5581 cgcaccccag ccacgcgctg gccgaacgcc tgctgcgcca tggcgcgcgc ggcgccggca
     5641 gcgtgttcag cttcgacctg aagggctcgc gcgcccaggg cagggtgttc atcgacaccc
     5701 tcaagctgtt cagccatctg gccaacgtgg gcgactgccg cagcctggtg atccacccgg
     5761 ccagcaccac ccacttccgc atgaacgacg aggcgctggc ggcggccggc atcgggccgg
     5821 gcaccatccg cctgtcgatc ggcctggaag atccgcagga cctgatcgac gacctcaagc
     5881 gggcgctgaa ggcggccgag aaagcggggg cctgagacca tgttcatcga cctccacggc
     5941 gctcccctgt acgcctacac cggcgggcgc cccttcgatc cggcgcagcc gaccgtggtg
     6001 ttcatccacg gcgtgctcaa cgaccacagc gtgtggatcc tgcagtcgcg cgccctggcg
     6061 caccacggct ggaacgtgct ggccatcgac ctgcccggcc acggccgcag cggcggccag
     6121 ccgccggcga cggtggaaca ggcggccgac gacgtgctgg ccctgctgcg cgcggccggc
     6181 atcgcccggg cggcgctggt gggtcacagc ttcggctcgc tgatcgcgct ggaagcggcg
     6241 gcgcgcgcgc cgtcccaggt cagccacctg gtgctggtgg gcacggcctt tccgatggcg
     6301 gtttcgccgg cgctgctgga agcttcggtc agcgcgcccg aaaaagccat ccacatggtc
     6361 aacgtgttct cgcacagcac gctgtgcccg ccgccgtcgg ccctgggccc gggcacctgg
     6421 ctgtacggca gctcgcgcgc cctgatgcgg cgggtgctgg ccagcaaccc gacggtcaat
     6481 ctgtttcaca ccggctttca ggcctgcgac cgctaccgcg gcggcgaaca ggccatggcg
     6541 gcggtgcagt gcccgacgct gctgctggtc ggtcgccacg accagatgac gccgccgcgc
     6601 gccgcgcgtg ccctggccgc ccatgcaagg caggccacgg tggtggaagt ggaggccggt
     6661 cacgccctca tgaccgaagc gccggacgcg gtgctggacg ccctgcgcca gttcctgagc
     6721 aggacctgag ggccgctgcc gcgcggccct gaattactat tgatttgata gctgctggcg
     6781 ctttccagac gggcgccaga gcgcgatttg acttgatgtc tggtgtggcc tcctcaggcg
     6841 cagggcgcgc gcgccggggc gggccgccat gccgcctgca ggtgctggcg cagccagcgc
     6901 agcaggccgg cgcagtgccc ttcgtcggcc tgccagctga agctggctgg cggcacctgc
     6961 gccgccgggg tggccgccag gtgcatcggc tcgatcacca ccgagtcgcc ggcatccaac
     7021 gccagcgcca gaccggcgct cacgtacaga tcctgcgccg ggtggtcgac gtggcgcccg
     7081 cgccgcgtca cccacagcgc gccctgcgcc acgcgcaggg tgccggcggc gggcaggtgc
     7141 agggtgcggg cctggccggg ttgcagcgtg atgctcacgc cgcgcgacag tggcagacgg
     7201 ggcgaagaag gcaggcgggc gggtgacatg gcggctcctg aagcgacggt cgaggaatcg
     7261 atcgggtgac ccgatgatgg ccctggcgcg attgacagtc caatgaattg cggtcaggct
     7321 attgatgcat tcaaggcatc aatccagcat gttgcacgcg cccacccatc tgcgctcgcg
     7381 tcccatccag gtcggccacc tgcgcgcctt cgaggcggtg gcgcgccacc tcaacttccg
     7441 cctggcggcc caggagctgg cgctctccca gccggccgtc agccgccaga tccaggcgct
     7501 ggaggacgac gtgggcgtgc cgctgtttcg gcgccacacg cgcgccgtgg agctgaccga
     7561 agccggcaac cagctgctga ccgcggtggg gcaggcgctg ccgcgcctgg acgccaccgt
     7621 gcgccacatc cgccagagcg ccgggcggcg ccatgtgtcg gtcaccacct ttgcctcgtt
     7681 tgcctcgatg tggctgatcc ccaggctgga agcgttccag cgcgaccacc ccgacatcga
     7741 catccgcgtc gaggccagcg acacggcggt ggacctggag gtgaccgaca tcgacgtggc
     7801 cgtgcgctac gccccggccg agcgcatgcc ggcgccggcg cagcgcctgt tcggcgaatc
     7861 ggtcacgccc atggccagcc cctggctgct caggagcggc ccgccgctca agcgtcctga
     7921 agacctgctg cgccacaccc tgatcgaggc gggcgacgcc tacaccccgc acctgcaatg
     7981 gctgacctgg cggcgctggc ttgacgccaa cggcctgaag gacgcccagc ccaagcgctg
     8041 gctcagcttc aactacgcct accaaatggt gcaggcggcg ctgtcgggcc agggcgtggt
     8101 gctggcgcgc ccgccgctgg tggccgagaa cctggcgcgc ggcgatttgg tggaggtgtt
     8161 cgccgccggc gcctacccgc aggcgcggct gatatcgccc atggcctact gggtgctggt
     8221 gtcgccgcac agccgccagc gtgccgaggt gcgcgccttt gccgactggc tgctgacgca
     8281 ggcgcaaagc acgcgctagt tgcggtggtt cgcgctgtcg ggcgctcata gaagccagaa
     8341 gaaggcttgc ggcggcgttg cgcctgttgc caaggccgcc agccttggct gcgagccgcg
     8401 ccttgccgca agccactttc tggcttcccg cagggcgcgc cgccaagggg ccatctgctt
     8461 ggtggcgtgg ccttgacatg gctccaccat gcctgcggcc ggcggcggcg gctacagccc
     8521 gtccagttcg cggtggcggc gcagcgtggt ccaggacgac cccagttcgg ccgccagctg
     8581 ctccaccagg tagaccgagc ggtgttgtcc gccggtgcag ccgatggcca cggtgacgta
     8641 gctgcggtgg tcgcgcgcca gcgccggcag ccagtggcgc aggaactgtg cgatctgggc
     8701 ctgcatgcgc tgcacctcgg gctgctgcgc caggtagtcg atcaccggct ggtcgcgccc
     8761 ggtgagtggc cgcagctcgg gcaggtagta cgggttgggc agcatgcgca cgtcgaacac
     8821 gtagtcggcg tccagcgtga tgccgcgctt gaaggcgaac gattcgaaca ccagcgtcag
     8881 gtgcgccagc ggggcggcga tcagttcctt gacggtggcc agcagctgcg agggcagcag
     8941 cacgctggtg tcgatcacca gcgagcgctc gcgcaggtcg gccatctggg cgcgttcgcg
     9001 cgcgatggcc tgcagcagcg cctgctgcac gtcgcccggg tcggccagcg acagcgggtg
     9061 ccggcgccgg gtttcggaaa accggcgcac cagggtgtga tcgttggctt ccaggaagat
     9121 ctggcgcagg tgcacgccgc gcgcggccag ctcgtcgagc agcggcggca gttgcggcag
     9181 cgactcggcg ctgcgcacgt ccatggcgat ggccagccgg cgcgcccgcc gctggtcctc
     9241 cagggcgatg aaggggcgca gcagctcggg cggcaggttg tcgacgcagt agtagccggc
     9301 gtcttccagc gcccgcagcg cgatcgactt gcccgagccg gacatgccgg tgatgaccac
     9361 cagctcggtg cccgtgggcg gcggggcggc ggccgccgag ggtgccggcg cgaagggggt
     9421 catgcgcgca cctttcgtgg ccgcgccgcg gcgcccggcg gctgggccgg cagcgccagc
     9481 atttctcgcg catgcgccag cgtggtgtcc gacagctgct cgccgcccag catgcgggcg
     9541 atttcgcgcg tgcgcgcctc gccgaccagg ggcgccacct ggctggtggg caggccgtcg
     9601 ccggtctgct tgctgacctg caggtgcgcg tcggcacagg cggccacctg cggcaggtgg
     9661 gtgacgcaca gcacctgccg gtcgcgcccc aggcgctgca gcagccgccc cacggtggcg
     9721 gccacggcgc cgccgacgcc ggcgtcgacc tcgtcgaaca ccagcgtgcc ggccgtgccc
     9781 aactggctgg tggtgacggc aatggccagg gccaggcgcg acaactcacc cccggatgct
     9841 acacgttcga tagcgcgtgg cgcagatccg gcgtgcgccg caaccagaaa tgccacattt
     9901 tccagaccgt gcggcgcggg ctcggccagc gcctgcagct ggacctcgaa gcgaccgcca
     9961 gccatgccca gcagctgcat cgcctcggtg acggcggcgg ccagctgcgg cgcggtctgg
    10021 cggcgctggc gcgacacggc gtcggcgctc tgccggtagg tttgctgcgc ggcgtcggcg
    10081 cgcgcctgca gctgcgccag atcggcggcg gcgtcgagct gcgccagctc ggtgcgccat
    10141 tgcgccagca ctgtgtgcag ctcctgcggc gggcgccgga agcgccgcgc caggcccagc
    10201 cactggccca ggcgctggtc cagcgccagc agctgctcgg ggtcgaggtt ggcgcgccgc
    10261 aggtagccgt gcaggctgtg ggcggcgtcg cgcgcctggg cgatggcgtc gtcgagctgt
    10321 tgggccaggg tggcgaactc gggttcgatg tcgcgccgcg cggccagcgc gtcgtgcgcg
    10381 tgccccagcg ccggcagggc accgccgtcg ccgtcgtcgc cttccagcgc ggccagcgcg
    10441 gcctgggcgg cgtcgatcag cgcctgggcg tgcgacagcc gggcgtggcg ctggttcagc
    10501 tcgtcccact cgccctcgcc gggcgccagt ttgtccagct cgccgatctg ccacagcagg
    10561 cgttcgcgtt cctgcgccag ctgttcctgc gcgctgcgcg cggcctgcag cgccgtgtcg
    10621 gcctggcgcc aggcctgcca ggcggtggtc agcggccggg tgtcgatgcc ggcgtaggcg
    10681 tcgagcaggg cgcgcacggc ggccgggcgc gtcaggctct gccaggcgtg ctggccatgg
    10741 atgtcgacca gccactcgcc cagcgtgcgc agctgcgcca gggtggcggg cgagccgttg
    10801 atccaggcgc ggctcttgcc ggcggcgtcg atggtgcggc gcagcagcag ggcgtcggcg
    10861 tcggtgtcga agccgtgctc ggccagccag gggcgggccg cgtcgggcag ttcgaactcg
    10921 gcggcgatgt cgcagcgcgg ctggccctcg cgcacccaca gggcgtcgcc gcgcccgccc
    10981 agcaccagtt gcagggcgtc gatcaggatc gacttgccgg cgccggtctc gccggtcagg
    11041 gcggtgaagc catcggccag cgccagatcg agcgccgcaa cgatgacgaa gtcgcgcaga
    11101 ctgaggtggc gcagtgccat ggggtcagac gcctccggtg ttccagtgca gcttgccgcg
    11161 cagggtgtcg aaatagctcc agccgcgcgg gtgcagcagg cgcatgcggt ggtcggagcg
    11221 tcgcaccacg atgcggtcgc cgtggtgcag cgaggtgatc gattgcatgt cgaagctggc
    11281 gctggcgtcg cgcccggcca cgatctcgat ggcgatctcg accgaatcgg ccagcacgat
    11341 cggccggttg gacagcgtgt gcggtgcgat cggcaccagc gcccaggccg gcaccatggg
    11401 gtgcagcagc gggccgccgg ccgacagcgc gtaggcggtg gagccggtgg gcgtggccac
    11461 gatgatgccg tcggcccggt ggttggcgac gaagcgccca ttgacctcca cccgcagctc
    11521 gaccatgctg gccaccgcgc cgcggttgac caccacgtcg ttcatggcca gcgcctcgaa
    11581 cacgcattgg ccgtcgcgca tcacctggcc gtgcatcagg ctgcgtgggt cttcttcgta
    11641 gccgcccgtc agcatggtgg gcagcacgcg gcggtagtcg cccagcggaa tgtcggtgat
    11701 gaagcccagg cgcccctggt tgatgccgat cagcggcgtg ccgtggcgcg ccaactggcg
    11761 cccgatgccg agcatggtgc cgtcgccgcc caccaccacc gccagatcgc agtcggtgcc
    11821 gatctgcggg acatcgagcg ccgggtactg ccgcaggccg gtggacaccg ccgtgcgctc
    11881 ggccagcgcc acctcgcagc cctgttccac cagaaaaccg gcgatgtcgg caatcacctg
    11941 gcgggtcgat tccggcgcgc tgccggtagc cgcatcctgg tatttgccga ccagcgcgac
    12001 acggtggaag tgaaggctca tgagcaaatt acatcataat gattttgaat gctggacgac
    12061 cgcgcacgcc tgctgctgaa gacgctgatc gagcgctaca tcgccgatgg tcagccggtg
    12121 ggttcgcgca cgctgtcgcg cgcgtcgggg ctggagctgt cgcccgccac catccgcaac
    12181 gtcatgtccg acctggagga gctcggcctg atcgccagcc cgcacacctc ggccggccgg
    12241 gtgccgacgg cgcgcggcta ccggctgttc gtcgacacca tgctgaccgc ccagcgcgcc
    12301 gagctggagg cccccgaact gcccgccgcc cagccgcaga aggtgattgc gcaggcggcg
    12361 cagctgctgt ccagcatgtc gcagttcgtc ggcgtggtga tggcgccgcg gcgttcgtcg
    12421 gtgttccgcc acatcgaatt cctgcgcctg tcggagcggc gcctgctgct gatcatcgtc
    12481 gcccccgacg gcgacgtgca gaaccgcgtg ctgttcaccg acacggacca cacggcgtcg
    12541 gagctgcaac aagcctccaa ctacctgaac gcccatttct ccggcctgga catcgaacag
    12601 gtgcgcctgc ggctgcggca tgaggtcgac agcctgcgcg gcgaaatcgc caagctgatg
    12661 cacgccgccg tgcaactggg cagcgaggcc atgacacagg cgcaggacga ggtggtcatc
    12721 tcgggcgagc gcaacctgct ggcggtgagc g
//
LOCUS       DAOOOG010000063        12704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_5461_length_12704_cov_28.312515, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000063 DAOOOG010000000
VERSION     DAOOOG010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12704
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5461_length_12704_cov_28.312515"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PK242_06435"
     CDS             <1..695
                     /locus_tag="PK242_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOZ93691.1"
                     /translation="TEPLMYQGGSDDFLGPCDDIRVADEAFGIDFEGELAVVTGDVPM
                     GATPEQGLEAVRLLMLANDVSLRQLIAAEVETRLGLVQGKPSTAFSPVAVTPDELGEA
                     WQDGRVHLTLQVSWNGRKVGLCEAGPDMRFHFGQLIAHAARTRRLRAGSIVGSGTVSN
                     PGVDQGGRRDWPKGYGCIAEKRAMETLQDGAPRTDYMRFGDTVRIEMKGRDGRPLFGA
                     IDQKVVQQGSGA"
     gene            879..1676
                     /locus_tag="PK242_06440"
     CDS             879..1676
                     /locus_tag="PK242_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOZ93692.1"
                     /translation="MNTPTLHTLRLDIDGDAVRATLCRPERLNAVGAQLLDELVVLAD
                     WLGEREDLHYLVLDHEGPVFSAGANLEEARELLADAGRARARLRANQRAAQQALIKLA
                     ALEQISFAALRGSAYGAGVAIAMACDFRVMAEDAVLNLPETRLGMFLSYGATPPLVAA
                     LGLARAKEMILFAEDWDAARCLAAGAVQQVAARGQVGARIDAMIATLRQRHWPAVRLA
                     KQVANAAAPARWGEVTLTEPELAAEALAGGDVAARVEAFLRRHHPER"
     gene            complement(1765..2367)
                     /locus_tag="PK242_06445"
     CDS             complement(1765..2367)
                     /locus_tag="PK242_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOZ93693.1"
                     /translation="MNDSFASATILLLLITDPVGNLPIFTHALRAVPPTRRRRVILRE
                     VLIAFALLLVFMFLGDGFLRLMNLSPLALQLAGGVVLFLIALRMVFPSAQPPDEPAPT
                     EPLIVPLAIPALAGPSALATVMLLVSQAPERRWEWVGALSVTMAVCALVLLLAERLQQ
                     RLGPRVLMAFERLMGLILVAVAVEMLLRAFRTLAAQLGGG"
     gene            2741..3577
                     /locus_tag="PK242_06450"
     CDS             2741..3577
                     /locus_tag="PK242_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ93694.1"
                     /translation="MHAWPTALFVLLWSSGAIVSRWGLDHASPFILLTMRFALALLVL
                     AALGWRTRAWLPAPGTAAHVAAVGLLLVGGYSCSYFLALDQGLTPGALATMLGVQPIL
                     TLLLQERRVTPMRLLGLLLALGGLVLVVKDSLLRAQVSLAGIALALLALACITLGAMA
                     QQRLAQSPIRVLPLQCAVALLLCLALVPTQPLRLEVGLPLLACVLWLGAVISVGATVL
                     LYRMLQAGNLVNVTSLFYLVPGGTLLLDAWLLGHPVTPLALGGLALVVGGLALVLRTR
                     PA"
     gene            3633..4442
                     /locus_tag="PK242_06455"
     CDS             3633..4442
                     /locus_tag="PK242_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOZ93695.1"
                     /translation="MSQPAIDYSRYQTFRISRRGEGGCVLDLQMKAANGKLPTAGHEG
                     HWELAEIWRDIDKDDSVRAVVLRGEGMGFSGGGDLALVQDMASDFAVRSRVWKEARDL
                     VYNVIHCEKPIVSAMHGPAVGAGLVAGLLADISIAARSARIVDGHTRLGVAAGDHAAI
                     CWPLLCGMAKAKYYLMLCEAVTGEEAERIGLVSLAVDDDQLLAKAYEVADKLAAGSTT
                     AIRWTKYSLNNWYRQAGPSFDTSLALEFMGFGGPDVHEGVASLRERRPPRY"
     gene            complement(4519..5169)
                     /locus_tag="PK242_06460"
     CDS             complement(4519..5169)
                     /locus_tag="PK242_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HOZ93696.1"
                     /translation="MLKLCGFAASNYYNKVKLALLEKGVPFEEVTAWVGETDTTASPL
                     GKVPYLLTDDGPISESTVILEYLEQRYPETPLLPASPYQAAKVRELLRYIELHLELVA
                     RNLYPQAFFGGQVSDGLKQRIRPQLERHVAAFARLAKFGPYIAGEQFTLADCAAAVHL
                     PLVSGASKIIYGQDLLAALPVKDYLQRLNQRPAVQRVEAERRANIALMMARMQPKT"
     gene            5295..5657
                     /locus_tag="PK242_06465"
     CDS             5295..5657
                     /locus_tag="PK242_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93697.1"
                     /translation="MNPLRHAHRLARQVLVWFALALVVAVAAPLVRAQPFELLCGAGG
                     QIKAVVHTASGDTLELGAGHLDCPLCVPTGAPGPLPRLGLAGPAPACVPDPTAPALRL
                     APAPLPWQARAPPPTHFR"
     gene            5823..6290
                     /locus_tag="PK242_06470"
     CDS             5823..6290
                     /locus_tag="PK242_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HOZ93698.1"
                     /translation="MKRLAISLSALALCAAASAQVTITEPWVRGTVPQQKASGLFMRI
                     DAEQHVRLLGGSSPVAGVVEVHEMAMQGEVMKMRALSHGLEIAKGHSLVLKPGGYHVM
                     LMELKQQLKAGDRVSVTLTFEDVASKQRFTREVQAPVTALGGAQPAPAAHHRH"
     gene            6387..7232
                     /locus_tag="PK242_06475"
     CDS             6387..7232
                     /locus_tag="PK242_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93699.1"
                     /translation="MSDSSNFDFGKLIPGFDFLQNLATSAKGVAAGPVSALRNWVAPT
                     VSVEELDKRITELKAVLFWLEQNSTALKATIQALEVQKMTLATLAGMNLSMSEVAKAF
                     TVGSPAKPDAAAASAPAAPPHWPFGQAPAAAPAAEPEPEPEPEPEPEPPARARAPARK
                     SKAKATPAATAAAPAPALADPMQWWGALTQQFQQIAANAVREAARAVPEQPAAAPAAT
                     AAAATAKKPAARKAPAARKAARKPAAKKAASRAKAATEAAPPATETRGATLGWPLPPP
                     FKLGR"
     gene            complement(7719..8921)
                     /locus_tag="PK242_06480"
     CDS             complement(7719..8921)
                     /locus_tag="PK242_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ93700.1"
                     /translation="MRKTFLTPLVAALLAAGSLPARAADDVIRIGVLNDQSGLYSDFG
                     GSTSVIAARMAVEDFGGKVLGKPIEIVSADHQNKADVATSVARRWFDVDGVSAVADLT
                     NSAVALAVQQLAKGQGKVTLSSGPATTRLTNEDCSPTGFHWAFDTYSQAVGTAKAVVA
                     TGGKSWFILAADYAFGHQMAADLDRVVKESGGSVLGQVRAPTGTMDFSSFLLQAQSSK
                     AQIIGLANAGADTVNSIKQAGEFGIMSGGQKIAGLVVVISDVHALGLKNANGLLLTTA
                     FYWDRDDASRKWSKRFFDRTGRMPGMVQAGTYSSVLHYLKAMQAAGKTDGKAVADKMR
                     ELPVDDAFAKGKLRADGRLMHDMYLVEVKKPGESKGPWDYYKILRTIPAEDAAQPLSQ
                     SKCSLVKQ"
     gene            complement(9012..10274)
                     /locus_tag="PK242_06485"
     CDS             complement(9012..10274)
                     /locus_tag="PK242_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HOZ93701.1"
                     /translation="MTSTASPSSSPERVFGWREHTALWFSLGVGLLVMQVGAYLVPAM
                     GTRDAALAVVGGSILGAGLLAWVAWLGCTSGQTSAGLIRTAYGQGFARLPILLNVVQL
                     LGWATFELVVMRDGTRAIARQALGIDPGLVAPTLLWGVIVLLLLRGSMLTLVRRIVSR
                     FGLPLVVLSLIWLSVQFGLKLEAAGLDALWQRAGDGSMNTFQALDLVIAMPVSWLPLV
                     ADYARHGRSGRSALGGTWLGYTIANIWCYGLGVLVITVVGPGTDLVGALLLAQGGLIA
                     LGLILIDELDNTYGDLYSGSVSTHSLHGGLSLRSWGTALALVSIAFALVLPMHQLEPF
                     LLMLSSVFVPLFGTILGRLGTQPARLAEAPRGVHAAPAAIWLLGIVLYHLLAQWAPQF
                     GSALPTLAATFVLGALSARTGTRAMQPA"
     regulatory      complement(10329..10439)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10569..10880
                     /locus_tag="PK242_06490"
     CDS             10569..10880
                     /locus_tag="PK242_06490"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOZ93702.1"
                     /translation="MKLTSSQLAPLLADLPGWHFDAQRGGLIEREYRFRDFAQAFGFM
                     AQVAVVAESRNHHPEWFNVYNQVKVALTTHDVDGLSMKDIELARFMDRAAAALTQEQA
                     P"
     gene            10877..11770
                     /gene="phhA"
                     /locus_tag="PK242_06495"
     CDS             10877..11770
                     /gene="phhA"
                     /locus_tag="PK242_06495"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HOZ93703.1"
                     /translation="MNASHDDSRHGLADRSYRPERADWTIDQDWGRYSAAEHAVWKTL
                     FERQSRLLPGRACQAFVQGMRELPMRHDQIPRFDELSEALMKRTGWQVVAVPGLVPDE
                     VFFEHLAHRRFPAGQFIRRPDELDYLQEPDVFHDVFGHVPMLMHPVLAEFIQAYGQGG
                     LRAQKLGMLEQLARVYWYTVEFGLVLEQGAPRIYGAGIASSASESVFAVEGDSPNRIG
                     FELERVMRTRYRIDDFQESYFVLDNLDQLLQLAQIDFDPVYARLRSGPVYEPGEVLAS
                     DRVVSRGSGAYHAAKRAGGQP"
     gene            11767..12456
                     /locus_tag="PK242_06500"
     CDS             11767..12456
                     /locus_tag="PK242_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ93704.1"
                     /translation="MSGAQPAVLITGAGGALGGAVVAHFAQRGARLVLLERDGTALRR
                     QWGAGSGAQIVEADLLNREQVAAQLAGAGPIDVLCHLAGGFHMGEPVHATPAAAWDLM
                     MDLNARSLIHVAAAVVPGMLARGRGRIVTVGAAGALKGGASMGAYAASKSALMRLTES
                     MSAELRDAGINVNGVLPSTLDTPANRAAMPDADASRWVAPEALAEVIGFLASDAARAV
                     HGALIPVLGRV"
BASE COUNT         1914 a         4385 c         4525 g         1880 t
ORIGIN      
        1 acaccgagcc gctgatgtac cagggcggca gcgacgactt cctgggcccc tgcgatgaca
       61 tccgggtggc ggacgaggcc ttcggcatcg acttcgaggg cgagctggcg gtcgtcaccg
      121 gtgacgtgcc gatgggcgcc acgcccgagc aggggctgga ggccgtgcgc ctgctgatgc
      181 tggccaacga cgtcagcctg cgccagctga tcgccgccga ggtcgagacc cgcctgggcc
      241 tggtgcaggg caagccctcc accgccttca gcccggtggc ggtgacgccc gacgagctgg
      301 gcgaggcctg gcaggacggt cgcgtgcacc tgacgctgca ggtgagctgg aacggccgca
      361 aggtcggcct gtgcgaggcc gggccggaca tgcgctttca cttcggccag ctgatcgccc
      421 acgccgcgcg cacccggcgc ctgcgtgccg gcagcatcgt cggcagcggc accgtcagca
      481 accccggcgt ggatcagggc ggccggcgcg actggcccaa gggctacggc tgcatcgccg
      541 aaaagcgcgc catggaaacc ctgcaggacg gcgcgccgcg caccgactac atgcggttcg
      601 gcgacaccgt gcgcatagaa atgaaggggc gcgacggtcg cccgctgttc ggtgccatcg
      661 atcagaaagt ggttcaacag ggctctggcg cttgatggac aagcgtcaac agctattcaa
      721 acaatagcac tcagggcggc gccgaggcgc ggagtcaggt gcgcggccgg cgccacgggg
      781 cggcccctgt cctgcccgtg acacgcctgg ggccagtccc gatgcgcgcc cgcctgaact
      841 gcgctgcatt gggccagcct acacgctcac cgccgcccat gaacacgccc accctgcaca
      901 ccctgcgcct ggatatcgac ggcgacgccg tccgcgccac tctgtgccgc ccggagcggc
      961 tcaacgccgt cggcgcgcaa ctgctggacg agctggtggt gctggccgac tggctgggcg
     1021 agcgcgagga tctgcactat ctggtgctcg accacgaggg gccggtgttt tcggccggcg
     1081 ccaacctgga agaagcgcgc gagctgttgg ccgacgccgg ccgcgcgcgg gcccgcctgc
     1141 gcgccaacca gcgcgctgcg cagcaggccc tgatcaagct cgccgcgctc gagcagatca
     1201 gctttgcggc gctgcgcggc tcggcctacg gcgccggcgt ggccatcgcc atggcgtgcg
     1261 actttcgcgt catggccgag gacgcggtgc tcaacctgcc cgagacgcgg ctgggcatgt
     1321 tcctcagcta tggcgccacg ccgccgctgg tggcggcgct ggggctggcg cgcgccaagg
     1381 aaatgatcct gttcgccgag gactgggacg ccgccaggtg cctggccgcc ggcgcggtgc
     1441 agcaggtggc ggcgcgcggg caggtgggcg cgcgcatcga cgccatgatc gccaccctgc
     1501 gccagcgcca ctggccggcg gtgcgcctgg ccaagcaggt ggccaacgcg gcggcgccgg
     1561 cacgctgggg cgaggtcacc ctgaccgaac ccgagctggc cgccgaagcg ctggccggcg
     1621 gcgacgtggc ggcgcgcgtg gaggcctttc tgcgccgcca ccaccccgag cgctgagctt
     1681 gtgagcaaaa catggctctg gggcgctcca ggtgtgcgcc agaagctatg taaaagatag
     1741 cattcgaggc ggcgctcggc gctttcagcc gccacccagc tgcgccgcca gcgtgcggaa
     1801 ggcgcgcagc agcatctcca ccgccaccgc caccaggatc aggcccatca ggcgctcgaa
     1861 ggccatcagc acgcgcgggc cgaggcgctg ctgcaggcgc tcggccagca gcagcaccag
     1921 cgcgcacacc gccatcgtca cgctgagcgc gcccacccat tcccagcggc gctcgggcgc
     1981 ctggctgacc agcagcatca ccgtggccag cgccgacggg ccggccagcg ccggaatcgc
     2041 cagcggcacg atcagcggct cggtcggggc cggctcgtcg ggcggctgcg ccgacggaaa
     2101 caccatgcgc agcgcgatca ggaacagcac cacgccgccg gccagctgca gcgccagcgg
     2161 cgacaggttc atcaggcgca gaaagccgtc gcccagaaac atgaacacca gcagcagcgc
     2221 aaacgcgatc agcacctcgc gcaggatcac gcggcgccgg cgcgtcggcg gcaccgcccg
     2281 cagggcgtgg gtgaagatgg gcaggttgcc caccgggtcg gtgatcagca gcagcaagat
     2341 ggtggcggag gcgaagctgt cgttcatggc aacgggaggc gccgaagcgg gcgcatgggc
     2401 gcgattgtgt cgcgcggcgc gaggcatgac agatcgcagg cgccggtggt tgcttgggaa
     2461 gtcctcgcat tgttgcgccg atcaatcggc agcgattgtc caaccatcac tgttagctac
     2521 aaaaacacag tgatctggtc ttgcgatctg ctcttggtcg agcatcggct accatgcagc
     2581 ccatgtcttt tcatcacctg agcccgccga tcgccgtcgc cccgcctccg ggtgcgccgg
     2641 cgctgggctg aatccccggc gccgatcgat cggtttgcgg caccgcccgg tgtgggcggg
     2701 ctgccgtttt gttcgggctt ttcaggaaag gtgatgtccc atgcatgcat ggccgacggc
     2761 gctgttcgtg ctgctgtgga gcagcggcgc cattgtttct cgctgggggc tggatcacgc
     2821 ctcccccttc attctgctga cgatgcgctt tgcgctggca ctgctggtgc tggccgcgct
     2881 gggctggcgc acgcgtgcct ggctgccggc accgggcacg gccgctcacg tggctgcggt
     2941 cgggttgctg ctggtgggtg gctactcgtg cagctacttt ctggcgctgg accaggggct
     3001 gacgccgggc gcgctggcca ccatgctggg cgtgcagccc atcctcacct tgctgttgca
     3061 ggagcgccgc gtcacaccga tgcgcctgct gggcctgctg ctggccctgg gcggactggt
     3121 gctggtggtc aaggacagtt tgctgcgcgc gcaggtgtcg ctggcaggca tcgcgttggc
     3181 gctgcttgcg ctggcctgca tcacgctggg tgcgatggcg cagcagcgtt tggcccagtc
     3241 gcccatccgt gtgctgccgc ttcaatgcgc cgtggcgctg ctgctgtgtc tggcgctggt
     3301 accgacgcag ccgttgcgtc tggaagtggg cctgccgctg ctggcctgcg tgctgtggct
     3361 gggcgcggtg atctccgtgg gcgctaccgt gctgctgtat cgcatgctgc aggccggcaa
     3421 tctggtcaac gtgacgagcc tgttctatct ggtgccgggc ggcacgctgt tgctggatgc
     3481 gtggttgctg ggccatccgg tgacgccgct ggcgctcggc ggactcgccc tggtcgtggg
     3541 cgggctggcg ctggtgctgc gcacgcgacc ggcctgaatc ggcgcttcgc taccatgggc
     3601 gggttcagga caattcaccg gagactttct gcatgagcca gcccgccatc gactacagcc
     3661 gctaccagac ctttcgcatc agccgccgcg gcgagggcgg ctgcgtgctc gacctgcaga
     3721 tgaaggccgc caacggcaag ctgcccaccg ccgggcatga ggggcattgg gaactggccg
     3781 agatctggcg cgacatcgac aaggacgaca gcgtgcgcgc cgtggtgctg cgcggcgaag
     3841 gcatgggctt ttccggcggc ggcgatctgg ccctggtgca ggacatggcc agcgacttcg
     3901 ccgtgcgctc gcgtgtgtgg aaggaagcgc gcgacttggt ctacaacgtc atccactgcg
     3961 aaaaacccat cgtcagcgcc atgcacggcc cggcggtggg cgccggcctg gtggccggcc
     4021 tgctggccga catctcgatc gccgcccgca gcgcccgcat cgtcgatggt cacacccgcc
     4081 tgggcgtggc cgcgggcgat cacgccgcga tctgctggcc gctgttgtgc ggcatggcca
     4141 aggccaagta ctacctgatg ctgtgcgagg cggtgaccgg cgaagaggcc gagcgcatcg
     4201 gcctggtgtc gctggcggtc gatgacgacc aactgctcgc caaggcctac gaggtggccg
     4261 acaagctggc cgccggcagc accaccgcca tccgctggac caagtactcg ctcaacaact
     4321 ggtaccgcca ggccgggccc agcttcgaca cctcgctggc gctggaattc atgggttttg
     4381 gcgggccgga cgtgcacgaa ggcgtggcgt ccttgcgcga gcggcgcccg ccgcgctact
     4441 gagccgcgcg ccccacgaaa gctattaaaa acatagcttc cggcgcttgc tgcaagcgcg
     4501 ccgaaggctg attcggcttc aggtcttggg ctgcatgcgg gccatcatga gggcgatgtt
     4561 ggcgcggcgc tcggcctcca cgcgctgcac cgccggccgc tggttcaggc gttgcaggta
     4621 gtccttcacc ggcagcgccg ccagcaggtc ctggccgtag atgatcttgc tggcgccgct
     4681 gaccagcggc aggtgcacgg cggcggcgca gtcggccagc gtgaactgct cgccggcgat
     4741 gtaggggccg aacttggcca gccgggcgaa ggccgccacg tgcctttcca gttgcgggcg
     4801 gatgcgctgc ttcaggccgt cgctcacctg gccaccgaag aaggcctgcg ggtacaggtt
     4861 gcgcgccacc agctccaggt gcagctcgat gtagcgcagc agctcgcgca ccttggcggc
     4921 ctggtagggg ctggcgggca gcagcggcgt ttcggggtag cgctgctcca gatactcgag
     4981 gatcaccgtc gattcgctga tcggcccgtc gtcggtgagg aggtagggca ccttgcccag
     5041 cgggctggcg gtggtgtcgg tctcgcccac ccaggcggtg acttcttcga acggcacgcc
     5101 cttttcgagc agggcgagct tgaccttgtt gtagtagttg ctggcggcaa agccacagag
     5161 cttgagcatg atgagcgatc tccggtgacg ggaaaagggc ggggggcgac gcgccggcgg
     5221 cagcatagca gccgcggccc gcacttgatg tcgcgcaatg gtctgggtgg gggcctggcc
     5281 ataatgcgcg tcggatgaac ccgctgcgcc acgcccaccg ccttgcccgc caggtgctgg
     5341 tgtggtttgc gctggcgctg gtggtggcgg tggctgcgcc gctggtgcgc gcgcagccgt
     5401 tcgaactgct gtgcggtgcg ggcggccaga tcaaggccgt ggtgcacacc gccagcggcg
     5461 acacgctgga gctgggtgcc ggccacctgg actgcccgct gtgcgtcccg accggtgcgc
     5521 ccgggccgct gccccgtctg ggcctggccg ggccggcgcc ggcgtgcgtg cccgacccga
     5581 cggcgcccgc cctgcgcctg gcacccgcgc cgctgccctg gcaggcccgc gcgccgcctc
     5641 cgacccactt ccgatagcta ctgttttgat agctttcggc gctgacgggg cgggcgatag
     5701 gggctgattc gatcttcagt gccagaggcc cgggccgttg cgccggcggg gtaccgctgg
     5761 gggcgacgcg ccccacgggc ccgccactgc ctgtgggtcg aatgttcaga aaggttttct
     5821 ccatgaaacg tcttgccatt tccctgtccg ccctggcgct gtgcgccgcc gcctcggccc
     5881 aggtcaccat caccgaaccc tgggtgcgcg gcaccgtgcc gcagcagaag gcctcgggcc
     5941 tgttcatgcg catcgacgcc gagcagcacg tgcgcctgct gggcggcagc tcgccggtgg
     6001 ccggcgtggt cgaagtgcac gaaatggcca tgcagggcga ggtcatgaag atgcgcgcgc
     6061 tcagccacgg cctggagatc gccaaaggcc acagcctggt gctcaaaccc ggcggctacc
     6121 acgtcatgct gatggagctc aagcagcagc tcaaggccgg cgaccgcgtg tccgtgacgc
     6181 tgaccttcga ggacgtcgcc agcaagcagc ggttcacgcg ggaagtccag gccccggtca
     6241 ccgcgctggg cggcgcccag cccgcgcccg cagcgcacca ccggcactga ccccgggagg
     6301 ctgaagacgg tcacgcggcg cgccggaacg cgccatatgc gttaagctgc gcagcactct
     6361 tcaacgcctg atcgggagcg gtggccatga gcgattccag caacttcgac ttcggcaagt
     6421 tgatccctgg cttcgatttc ctgcagaacc tggccaccag cgccaagggt gtcgccgccg
     6481 gcccggtgtc ggcgctgcgc aactgggtgg cgcctaccgt cagcgtggaa gaactggaca
     6541 agcgcatcac cgagctgaag gcggtgctgt tctggctgga gcagaacagc accgcgctca
     6601 aggccaccat tcaggcgctg gaggtgcaga agatgacgct ggccacgctg gccggcatga
     6661 acctgagcat gagcgaggtc gccaaggcct tcaccgtggg gtcgcccgcc aagccggacg
     6721 ccgccgcggc cagcgcgccg gctgcgccac cgcactggcc gttcggtcag gcgccggccg
     6781 cagcaccggc cgccgagccc gaacccgaac ccgagcccga acccgagccg gaaccaccgg
     6841 cgcgtgccag ggcgccggcg cgcaagagca aggccaaggc caccccggcc gccactgcgg
     6901 ccgccccggc gccggccctg gccgatccga tgcagtggtg gggtgcgctg acgcagcagt
     6961 tccagcagat tgccgccaac gccgtgcgcg aggcggcgcg tgcggtgccg gaacagcccg
     7021 ccgcggcgcc ggcggccacc gcggcggccg ccaccgccaa gaagccggcc gccaggaagg
     7081 cgccggcggc ccgcaaagcg gcgcgcaagc cggcggccaa gaaagcggcc tcgcgcgcca
     7141 aggcggcgac cgaagctgca cctccggcca ccgagacccg cggcgccacc ctgggctggc
     7201 cgctgccgcc gccgttcaag ctcggtcgtt gacgtactag aaacggcagt taaccgctgg
     7261 tcaacttcgg gcagaccgca tcgatgcggg ggctcgcggc tgcgatggcc ttcacctcct
     7321 gggtgagcac gctgcgcacc cacccgcacc gcgctcggcg cgccatgcgg cgttgctcct
     7381 ccttgcgggc aggagcccgc gacatcgtcg cgtcgtgcct ggcacgccga aagcgttctc
     7441 gaagagcggc gcagccaaaa tcggccccag cgagaccagt ttttgccgga tttgcagccc
     7501 aatagctaag ctattgggca aaaagccggt gctctcgaag agcggcgaag ccaaaaatgg
     7561 gccgctgcgg tcgatttgca gccggcgatt tccaagtccg accgcctcct aggttgaacc
     7621 tgatcggctg cagccccggt acgtgacgct ggccgggcat ggccgcgccg atcatgcgct
     7681 gccgaaggcg cgccggcggc ggccggcgcg cccggggttc actgcttcac cagcgagcac
     7741 ttgctctggc tgagcggctg cgcggcatct tcggcgggga tggtgcgcag gatcttgtag
     7801 tagtcccacg gccccttgga ctcgcccggc ttcttgacct ccaccaggta catgtcgtgc
     7861 atcagccggc cgtcggcacg cagcttgccc ttggcgaagg cgtcgtccac cggcagttcg
     7921 cgcatcttgt cggccaccgc cttgccgtcg gtcttgccgg ccgcctgcat ggccttcagg
     7981 tagtgcagca ccgacgaata ggtgccggcc tgcaccatgc ccggcatgcg gccggtgcgg
     8041 tcgaaaaagc gcttggacca cttgcgcgag gcgtcgtcgc ggtcccagta gaacgcggtg
     8101 gtcagcagca ggccgttggc gttcttcagg cccagcgcgt ggacgtcgct gatcaccacc
     8161 accaggccgg cgatcttctg gccgcccgac atgatgccga actcgcccgc ctgcttgatc
     8221 gagttgacgg tatcggcccc cgcgttggcc aggccgatga tctgcgcctt ggacgactgc
     8281 gcctgcagca ggaaggagga gaaatccatc gtgccggtgg gggcgcgcac ctggccgagc
     8341 acgctgccgc cgctttcctt gaccacgcgg tccagatcgg ccgccatctg gtgcccgaac
     8401 gcgtagtcgg cggccaggat gaaccagctc ttgccgccgg tggccaccac cgccttggcc
     8461 gtgccgacgg cctgcgagta ggtgtcgaac gcccagtgga agccggtggg cgagcaatcc
     8521 tcgttggtca ggcgggtggt cgccggcccg gacgacaggg tcaccttgcc ctgccccttg
     8581 gccagctgct gcacggccag cgccacggcc gaattggtca ggtcggcgac ggcgctgacg
     8641 ccgtccacgt caaaccagcg gcgcgccacg gacgtggcga cgtcggcctt gttctggtgg
     8701 tcggccgaga cgatctcgat cggcttgccc agcaccttgc cgccaaagtc ttccaccgcc
     8761 atgcgggctg cgatcaccga ggtggagccg ccgaagtcgg aatacaggcc cgactggtcg
     8821 ttcagcacgc cgatgcggat cacgtcgtcg gcggcccggg cgggcaggct gcccgcggcc
     8881 agcagggccg cgaccagggg agtcagaaac gtcttgcgca tgcaccctct ccttgtgttg
     8941 aaagcgccga tcactatagg gcgggggccg gcgcggcgac accggggaat cccccgatcc
     9001 ggcgtactgg ctcaggccgg ctgcatcgcc cgcgtaccgg tgcgcgcgct cagcgcgccc
     9061 agcacgaagg tggccgccag cgtgggcagc gcgctgccga actgtggcgc ccactgcgcc
     9121 agcaggtggt agagcacgat gccgagcagc cagatcgccg ccggcgcggc gtgcaccccg
     9181 cgcggggcct cggccaggcg cgccggctgg gtgcccaggc gccccaggat ggtgccgaac
     9241 agcggcacga acaccgagct gagcatgagc aggaagggct ccagctggtg catcggcagc
     9301 accagtgcga aggcgatcga aaccagcgcc agcgccgtgc cccagctgcg caggctcagg
     9361 ccgccgtgca ggctgtgggt cgagaccgag cctgaataca ggtcgccgta ggtgttgtcg
     9421 agctcgtcga tcaggatcag cccgagcgcg atcaggccgc cctgcgccag cagcagggcg
     9481 ccgaccaggt cggtgccggg accgacgacg gtgatcacca gcacgcccag gccgtagcac
     9541 cagatgttgg cgatggtgta gcccagccag gtgccgccca gggcgcttcg gccgctccgg
     9601 ccgtggcgcg cgtagtcggc caccagcggc agccacgaca ccggcatggc gatcaccagg
     9661 tccagcgcct ggaaggtgtt catgctgccg tcgccggcgc gctgccacag cgcgtccagc
     9721 ccggccgcct ccagcttcaa cccgaactgc acgctgagcc agatcagcga cagcaccacc
     9781 agcggcaggc cgaagcggct gacgatgcgg cgcaccagcg tcagcatcga gccgcgcagc
     9841 agcagcagca cgatcacgcc ccacagcagc gtgggcgcca ccaggcccgg gtcgatgccc
     9901 agcgcctgcc gggcgatggc ccgcgtaccg tcgcgcatca ccaccagctc gaaggtggcc
     9961 cagcccagca actgcaccac gttgagcagg atcggcaggc gcgcaaagcc ctggccgtag
    10021 gcggtccgga tcaggccggc gctggtctgg ccgctggtgc agcccagcca ggccacccag
    10081 gccagcagcc cggcgcccag gatggagccg cccaccaccg ccagcgccgc atcgcgcgtg
    10141 cccatggccg gcaccaggta ggcgcccacc tgcatcacca gcaggccgac gcccaggctg
    10201 aaccacaggg cggtgtgctc gcgccagccg aacacgcgct cgggcgagga agagggggaa
    10261 gcagtggatg tcatggtgcg ccggtccgtc aaaaaaatgc aggaggcagg cgcgcggcgg
    10321 cccggcggcc gcccggcgag ctgctttcct gcgcgaggat gatctcaatc aggttcgaag
    10381 ggactgtctc agccgcaccg cgccgtcgaa acggccgcgc ggcaccccca gcgatgctcg
    10441 aagggggtcg attctgccgc agcggggccg gtgctgtcca tgaccctcgc ggcgacgggc
    10501 atgctgccgg cggcgccatc ggtcgacccg tggcgtggct atcatcgatc gactggaggg
    10561 acgtgcccat gaaactcacc tcgtcacaac tggcgcccct gctggccgac ctgcccggct
    10621 ggcacttcga tgcgcagcgt ggcggcctga tcgagcgcga gtaccggttc cgcgacttcg
    10681 cccaggcctt cggcttcatg gcgcaggtgg cggtggtggc tgaaagccgc aaccaccacc
    10741 cggaatggtt caacgtctac aaccaggtca aggtggcgct gaccacccac gacgtggacg
    10801 gcctgtcgat gaaggacatc gaactggcgc gcttcatgga tcgggcggcc gcggccctga
    10861 cgcaggagca ggcgccatga acgcgtcaca cgacgacagc cggcacggcc tggccgaccg
    10921 cagctaccgg cccgagcggg ccgactggac catcgaccag gactggggcc gctacagcgc
    10981 cgccgagcac gcggtctgga agacgctgtt cgagcgccag agccgcctgc tgcccggccg
    11041 cgcctgccag gccttcgtgc agggcatgcg cgagctgccg atgcgccacg accagatccc
    11101 gcgcttcgac gagctgtccg aggcgctgat gaagcgcacc ggctggcagg tggtggcggt
    11161 gcccggcctg gtgccggacg aggtgttttt cgaacatctg gcgcatcggc gctttccggc
    11221 cggtcagttc atccgtcggc cggacgagct cgattacctg caggagcccg acgtgttcca
    11281 cgacgtgttc ggccatgtgc cgatgctgat gcatccggtg ctggccgagt tcatccaggc
    11341 ctatggccag ggcgggctgc gcgcgcagaa gctgggcatg ctggagcagc tggcgcgggt
    11401 gtactggtac acggtggaat tcggcctggt gctggagcag ggcgcgccgc gcatttatgg
    11461 cgccggcatt gcgtcgtcgg cgtccgagtc ggtgtttgcg gtggagggcg attcgcccaa
    11521 ccgcatcggc ttcgagctcg agcgcgtgat gcgcacacgc taccgcatcg acgattttca
    11581 ggagagctat ttcgtgctgg acaacctgga tcagctgctg cagctggcgc agatcgactt
    11641 cgacccggtg tatgcgcggc tgcgcagtgg cccggtgtat gagcccggcg aggtgctggc
    11701 cagcgaccgc gtggtcagcc gcggcagcgg cgcctaccac gccgccaagc gcgccggggg
    11761 ccagccatga gcggcgcgca gcctgcggtg ttgatcaccg gcgccggcgg cgcgctgggc
    11821 ggcgcggtgg tggcgcattt tgcccagcgc ggcgcgcgcc tggtgctgct ggagcgcgat
    11881 ggcacggccc tgcgtcgcca gtggggcgcg ggcagcggcg cgcagatcgt cgaggccgac
    11941 ctgctgaacc gcgagcaggt ggcggcgcaa ctggccgggg cgggcccgat cgatgtgctg
    12001 tgccatctgg ccggcggctt tcacatgggc gagccggtgc acgccacgcc cgctgcggcg
    12061 tgggatttga tgatggacct gaacgcgcgc agcctgatcc acgtggccgc cgccgtggtg
    12121 ccgggtatgc tggcgcgcgg ccgtgggcgc atcgtcaccg tgggcgcggc cggcgcgctg
    12181 aagggcggcg ccagcatggg cgcctatgcc gcgtccaaga gcgcgctgat gcggctgacc
    12241 gaatccatgt cggccgagct gcgcgacgcc ggcatcaacg tcaacggcgt gctgccctcc
    12301 accctggaca cgcccgccaa ccgcgccgcc atgcccgacg ccgacgccag ccgctgggtc
    12361 gcgcccgagg cgctggccga ggtgatcggc tttctggcct cggacgcggc gcgcgccgtg
    12421 catggggcgc tgattccggt gctggggcgg gtctgagggt cgggtggcgc gaactccaag
    12481 cgcccgaaca tccgtgcgca gagggcgcaa agatcacgcc gaagacgcag aaagcatgca
    12541 tgtcatgagc tgttgtgcgc accctgcgaa tcttctgcgc tttctgcgca gggatgtctg
    12601 gttttcctgg agcaccacga accactacca cccctgacca gccgtcaccc gccgcagcac
    12661 gaagctgctc tcgcgggcgg gcacgccggg ctggggcagg gatt
//
LOCUS       DAOOOG010000064        12490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_5587_length_12490_cov_24.368637, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000064 DAOOOG010000000
VERSION     DAOOOG010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12490
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5587_length_12490_cov_24.368637"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PK242_06505"
     CDS             complement(<1..616)
                     /locus_tag="PK242_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOZ93705.1"
                     /translation="MTNAWVSEAVRRIERDFQRSADTHLFPIDLPALAGVQIYLKDES
                     THPTGSLKHRLARSLFLYGLCNGWIGEGTTIIESSSGSTAVSEAYFARLLGLPFIAVM
                     PRSTSAAKVAQIAFYGGRSHFVERGDQVYDEAARLARECGGHYMDQFTYAERATDWRG
                     NNNIAESIFQQMRAEPHPVPRWIVVGAGTGGTSATIGRYLRYQGH"
     gene            complement(613..1404)
                     /locus_tag="PK242_06510"
     CDS             complement(613..1404)
                     /locus_tag="PK242_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HOZ93706.1"
                     /translation="MKLRLVPTPTGVQWVRAGVRTFFRQPLALMGLFLMFLAVVSVLA
                     FVPFIGSVLALVAVPALSLGLMAAAREADEGRFPMPLTLFAALRKSPQASRAMVQLGL
                     LYAIGVMLVMGISALVDGGEFAQVYVQGGGLTPEKVMEPGFRSAMWLSTLLYLPLSLA
                     FWHAPALVHWHDVAPVKSLFFSLVAIFRNRKAYLLYGAMWLALSLGVGMVAMLLLAFV
                     LRFSGLILAATAMLPLSLVLAAIFFTSQWFTFRDSFDADSESTIP"
     gene            complement(1401..2387)
                     /locus_tag="PK242_06515"
     CDS             complement(1401..2387)
                     /locus_tag="PK242_06515"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOZ93707.1"
                     /translation="MAVYTEVSEDEARALLQRLGLGELTALRGIEGGIENTNYFASTT
                     EGEWVLTLFERLTHAQLPFYLHLMKHLAQHGVPVPDPQAKPAGLPVPPGEDDLLHTLH
                     GKPTAVVNRLRGKSELSPGPAHCAAVGDMLARAHLAARDYDRRQPNLRGLAWWNETVP
                     VVLPFLDESQATLIRSELAYQNHVAAGSAYAALPRGAVHADLFRDNVMFEGTAEQPVL
                     TGFFDFYFAGVDTWLFDLAVTLNDWAIDLPTGATDPARTAALLHAYQAVRPLTDAERR
                     LLPAMLRAGALRFWLSRLWDFHLPREASLLKPHDPRHFERVLRQRLAAPLTA"
     gene            complement(2419..2766)
                     /locus_tag="PK242_06520"
     CDS             complement(2419..2766)
                     /locus_tag="PK242_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93708.1"
                     /translation="MRPSPALPIALAACGLLIGTLVSAQTPGGAAASSLRDAPVLDSR
                     TQRVEHIQHEDAGSRVSELRVGGETRSISVQPKAGVPAYEVRPADASGTSAVQREAGP
                     GSAGPRVWKMLQF"
     gene            2813..5584
                     /gene="polA"
                     /locus_tag="PK242_06525"
     CDS             2813..5584
                     /gene="polA"
                     /locus_tag="PK242_06525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOZ93709.1"
                     /translation="MSSDPTPATAPRLLLVDGSSYLYRAFHAMPDLRADPNDRHSQPT
                     GAIRGMINMLNALRKDYPDAPYAACVFDAPGKTFRDELYPDYKAQRAPMPDDLRSQIA
                     PIHEVVRLLGWKVLAVPGVEADDVIGTLAQAAAGQGVEVVISSGDKDLAQLVTPHIVI
                     VDTMTGKRRDMAGVQAEFGVRPDQMLDYQVLLGDAIDNVPGVDKVGPKTAARWLGEYG
                     SLDAIVAQADRIKGAAGDNLRRALAWLPTGRRLLQIRTDCDLTGHVDGLPALDSIAAG
                     ASDTSALAEFYDKYGFRTLARALRPAAAGDAAPNAAPVPAAAATGGHDVAYETILDWP
                     AFERWLARIEAAELTALDTETTSIDEQQARIVGLSLSVRPREAAYIPLAHDGPGAPAQ
                     LPLGEVLARLKPWLENPARTKLGQHVKYDRHVLANHGIEVAGYVHDTMLQSYVLEVHR
                     PHGLASLAERHLGRRGIDYEALCGKGVHQIPFAQVPIDKAAHYACEDADQTLEVHLAL
                     WPQVQAEPGLARIYALEIATSEVLYRIERHGVLIDAAQLQRQSHELGRRILELERQAH
                     ELAGQPFNLGSPKQIGEIFFDKLGLPVVKKTATGKPSTDEEVLEKLAEDYPLPARILE
                     HRGLAKLKSTYTDKLPQLVDPVSGRVHTHYAQAVAVTGRLASNDPNLQNIPVRTPEGR
                     RIREAFIAPPGHVVVSCDYSQIELRIMAHLSDDAALLAAFHAGMDVHRATAAEVFGVA
                     PEQVSSEQRRYAKVINFGLIYGMSSFGLARNLGIDNTAARNYIDRYFERYPGVRRYMD
                     DTRQAAKARGFVETVFGRRLYLPEINSPNGPRRAAAERAAINAPMQGTAADLIKMSMV
                     AVQAAIDQQRRASRMIMQVHDELVLEVPEAELAWARAEVPRLMAGVAQLKVPLLAEVG
                     MGPNWEQAH"
     gene            complement(5661..6680)
                     /locus_tag="PK242_06530"
     CDS             complement(5661..6680)
                     /locus_tag="PK242_06530"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOZ93710.1"
                     /translation="MSSPISLSRYLVEKQRIAGHIPAQLRLLLEVVARACKSISHAVN
                     KGALGGVLGSAGSQNVQGEVQKKLDIIANEVLIDANEWGGHLAAMASEEMEAIHVVPN
                     RYPKGEYLLLFDPLDGSSNIDVNVSIGTIFSVLKMPEGDRGVEEEDFLQPGSHQVAAG
                     YCVYGPQTTLVLTVGDGVAMFTLDRELGSFVLTQDDLRIPDDTQEFAINMSNLRHWDA
                     PVRRYIDECLAGKDGPRGKNFNMRWVASMVADVHRIMMRGGVFMYPWDKREPNKPGKL
                     RLMYEANPMSWLIEQAGGAATNGRQRILDVQPQQLHERVSVILGSKNEVERVTRYHLD
                     ADAAS"
     gene            complement(6677..9436)
                     /gene="pepN"
                     /locus_tag="PK242_06535"
     CDS             complement(6677..9436)
                     /gene="pepN"
                     /locus_tag="PK242_06535"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HOZ93711.1"
                     /translation="MREGQTAVTRRQDYAPPAFWIDSVELTIDLDPAKTRVLSRLRLR
                     RNPDVAAQPLRLDGDELNLSRVMLGGQACSFRLDGQTLVLEQLPADHAPFELEIFTTC
                     APEKNSKLMGLFMSGGDFFTQCEAEGFRRITYFLDRPDVMATYTVTLRADKARWPVLL
                     ANGNLVDAGELDDGRHFATWHDPHRKPCYLFALVAGNLVAREQRITTRTGSEHLLQVF
                     VRPGDLDKTEHAMRSLIHAVAWDEARFGLPLDLERFMVVATSDFNAGAMENKGLNIFN
                     TKYVLAKPSTATDGDYDAVESVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEF
                     SEDLAGTPSARAVKRIENVIALRAAQFPEDASPMAHPVRPDEYLEISNFYTTTIYEKG
                     AEVVRMIQSLLGRAGFRRGMDLYFQRHDGQAVTCDDFAQAMADANPGSTLAPLLPQFK
                     RWYSQAGTPVLQATGQYDAQARRYTLTLSQSCPATPGQPHKAPFVIPVRLGLVGADGR
                     ELPLTLSRGELLHGDTCVLTEPQTTLVFSEVDQPPVPSLLRGFSAPVELRGLDADEQL
                     LHLLAYDSDPFNRWDAGQRLGVRIALENIASGADQVSARPLKDNYLQALRAVLRQPQL
                     DPAFKALVLKLPDEDYIADQLDVVDPQRIHAVRDAMQRQLATALRDDWQWAWEQHHDT
                     GAYRPDPHSSGRRALAGLALAMLCLAASEQGDSVWPGKAYQRVKDAGNMTDRLNALGA
                     LVGCGHELGAHALSRFHRLFRHEPLALDKWFALQARAPDRRGGALPTVRLLMKHPDFS
                     LRNPNRARSLIFAYCHNNPAGFHRRDAAGYLFWADRVLELDTLNPQVAARLARAMDAW
                     ARLAEPYRSAAREAIARVAARPDLSNDVREVVTRALAGQAPALAADAEARTTETPDTQ
                     QKGSA"
     gene            complement(9521..10625)
                     /gene="prfB"
                     /locus_tag="PK242_06540"
     CDS             complement(join(9521..10543,10545..10625))
                     /gene="prfB"
                     /locus_tag="PK242_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123395.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOZ93712.1"
                     /translation="MEAERLNQISAQIDDLRARTDALRGYLDYAGKAERLRTVNAALE
                     DPAVWNDPRKAQELGKEKKQLDGVVVTLDELTQGLTDSAELFELSREENDDASLQAIG
                     SDVEKLARTVEELEFRRMFNQPADPNNCFIDIQAGAGGTEACDWASMLLRQYLKYAER
                     KDFNTEIQEETPGDSAGIKSATIKVEGEYAYGLLRTETGVHRLVRKSPFDSSGGRHTS
                     FASVFVYPEVDDSIEIDINPADVRTDTYRASGAGGQHINKTDSAVRLTHIPTGIVVQC
                     QDSRSQHSNRDVAWQRLRARLYDHEMRKRMEDQQKLEDSKTDVGWGHQIRSYVLDNSR
                     IKDLRTGVEISATQKVLDGDLDPFIEASLKQGV"
     gene            complement(10834..11763)
                     /locus_tag="PK242_06545"
     CDS             complement(10834..11763)
                     /locus_tag="PK242_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ93713.1"
                     /translation="MSYVPHRPARSESLPLRQIHVHVNCWGRPQPGSTPLVLVHGWMD
                     VGASYQFMVDALSAEFADQRLIIAPDWRGFGDTTLAAPCDHYVLADYLGDLDALLDHY
                     APNGQPVDLVGHSMGGNVVMQYAGARPARIRRLVNLEGFGMPASRPTQAPRRLAQWLD
                     ELQQLRRGEISLKTYPDADAVAQRLMKTNPRLPADKAAWLARQWARPGADGQWRILGD
                     AAHKIVNPSLFRVDEMLALYAAITAPVLVIEGSDDSLSQWWKGRYTLAEFHQRLQAVP
                     DLRRQQLPDSGHMLHHDQPERLAAALQDFLSPP"
     gene            11923..>12490
                     /locus_tag="PK242_06550"
     CDS             11923..>12490
                     /locus_tag="PK242_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOZ93714.1"
                     /translation="MKHKLLVMALGAALAAPAAIAGTVTVLTSFPKELTTAYKKAFES
                     AHPGITVEILNKNTTASVAYIRELPEGQRPDVMWASAPDAFEVLSSFKLLQSAPEVVN
                     KSAPAKIGNYPLNDPKGMYYGQALAGYGISWNTRYLKAHKLTAPKEWSDLVRPEYFGH
                     IAISSPSRSGTTQLTVETILQGEGWDKGW"
BASE COUNT         1877 a         4130 c         4463 g         2020 t
ORIGIN      
        1 cgtggccctg gtagcgcagg tagcggccga tggtggcgga ggtgccgcca gtgcccgcgc
       61 cgaccacgat ccagcgcggc accgggtgcg gctcggcgcg catctgctgg aagatcgatt
      121 cggcgatgtt gttgttgccg cgccagtcgg tggcgcgctc ggcataggtg aactggtcca
      181 tgtagtggcc gccgcattcg cgcgccagcc gggcggcctc gtcgtacacc tgatcgccgc
      241 gctcgacgaa gtggctgcgc ccgccgtaga aggcgatctg cgccaccttg gcggccgagg
      301 tgctgcgtgg catcacggcg atgaagggca gaccgagcag gcgcgcgaag taggcctccg
      361 acaccgccgt gctgcccgag gacgattcga tgatggtggt gccctcgcca atccagccat
      421 tgcatagccc gtacagaaac agcgagcgcg ccagccggtg cttgaggctg ccggtggggt
      481 gggtggattc gtccttcagg tagatctgca cgccggccag tgcgggcaga tcgatcggaa
      541 acaggtgggt gtcggccgaa cgttgaaagt cgcgctcgat gcgccgcacg gcttcgctga
      601 cccaggcgtt ggtcatggga tagtgctttc ggagtcggcg tcgaagctgt cgcggaaggt
      661 gaaccactgc gaggtgaaga agatcgcggc cagcaccagc gaaagcggca gcatggcggt
      721 ggccgccagt atgaggccgc tgaaccggag cacgaaggcc agcagcagca tcgccaccat
      781 gccgacaccc agcgacagcg ccagccacat cgcgccgtac agcaggtagg ccttgcggtt
      841 cctgaagatg gccaccaggc tgaagaacag gcttttcacc ggcgccacgt cgtgccagtg
      901 caccagcgcg ggcgcgtgcc agaacgccag cgacagcggc aggtacagca gggtggacag
      961 ccacatcgcg ctgcgaaagc ccggctccat cactttctcg ggggtgaggc cgccgccctg
     1021 cacgtagacc tgcgcaaact cgccgccatc gaccagggcc gaaatgccca tcaccagcat
     1081 cacgccgatg gcatacagca ggcccagctg caccatggcg cggctggcct gcgggctctt
     1141 gcgcaaggcc gcaaacagag tcagcggcat gggaaatcga ccctcgtcgg cttcgcgcgc
     1201 ggcggccatc aggcccagcg agagcgccgg cacggccacc agcgccagca cgctgccgat
     1261 gaagggcacg aaggccagca ccgacaccac cgccagaaac atgaggaaca gccccatcag
     1321 cgccagcggt tggcgaaaga aggtgcgcac gcccgcgcgc acccactgca cgccggtcgg
     1381 ggtggggacc aggcgcagct tcatgccgtc agtggcgccg ccaggcgctg gcgcagcacg
     1441 cgctcgaagt ggcgcgggtc atgcggtttg agcaggctgg cttcgcgcgg cagatggaag
     1501 tcccacaggc gcgacagcca gaagcgcagg gcgccggcac gcagcatggc aggcagcagg
     1561 cgccgctcgg cgtcggtgag tgggcgcacc gcctggtagg cgtgcagcag cgcggccgtg
     1621 cgcgccgggt cggtggcgcc ggtgggcagg tcgatggccc agtcgttgag cgtaaccgcc
     1681 aggtcgaaca gccaggtgtc gacgccggca aagtagaagt cgaaaaaacc cgtcagcacg
     1741 ggttgttcgg ccgtaccctc gaacatcacg ttgtcgcgaa acaggtcggc atgcaccgcg
     1801 ccacgcggca gcgcggcgta ggcgctgccg gcggcgacat ggttctggta ggccagctcg
     1861 ctgcggatca gcgtggcttg cgattcgtcc agaaacggca gcaccaccgg caccgtctcg
     1921 ttccaccagg ccagcccgcg caggttgggc tggcgccggt cgtagtcgcg tgccgccagg
     1981 tgggcgcgcg ccagcatgtc gcccacggcg gcgcagtgcg ccgggccggg gctgagctcg
     2041 ctcttgccgc gcagacggtt gaccaccgcc gtgggcttgc catgcagggt gtgcagcagg
     2101 tcgtcttcgc ccggcgggac gggcagcccg gcgggcttgg cctgcggatc gggcaccggc
     2161 accccgtgct gggccaggtg cttcatcagg tgcaggtaaa acggcagctg cgcgtgcgtc
     2221 aggcgctcga acagcgtcag cacccattcg ccctcggtcg tgctggcgaa gtagttggtg
     2281 ttttcgatgc cgccttcgat gccgcgcagc gcggtcagct cgcccaggcc cagccgctgc
     2341 agcagggcgc gggcctcgtc ttcggagact tcggtgtaaa ccgccatgtc gcgcttgtgg
     2401 ggtgtgcggc gtgcgctatt aaaactgaag catcttccag acccgcgggc cggcgctgcc
     2461 ggggccggct tcgcgctgca cggcgctggt gccgctggcg tcggccgggc gcacttcgta
     2521 ggccggcacg ccggccttgg gctgcacgct gatgctgcgg gtttcgccgc ccacgcgcag
     2581 ttcgctgacg cggctgccgg catcttcatg ctggatgtgt tccacgcgct gcgtgcggct
     2641 gtcgagcacc ggggcatcgc gcagcgacga ggccgcggcg ccgcccggtg tctgggccga
     2701 caccagggtg ccgatcagca gaccgcaggc ggccagcgcg atggggaggg caggggatgg
     2761 gcgcatagcc tcattttatg gcgcagcgct gcgccgcagg cacaatgccg cgatgagcag
     2821 cgaccccaca cccgccaccg cgccccgcct gctgctggtc gatggctcca gctacctgta
     2881 ccgggcgttt cacgccatgc ccgatctgcg cgccgatccg aacgatcgtc acagtcagcc
     2941 caccggcgcg atccgcggca tgatcaacat gctcaacgcg ctgcgcaagg actaccccga
     3001 cgcgccctat gcggcctgcg tgttcgacgc ccccggcaag accttccgcg acgagctgta
     3061 ccccgactac aaggcccagc gcgcgcccat gcccgacgac ctgcgcagcc agatcgcgcc
     3121 gatccacgag gtggtgcgcc tgctgggctg gaaggtgctg gcggtgccgg gcgtggaggc
     3181 cgacgacgtg atcggcacgc tggcgcaggc ggcggcgggg cagggcgtgg aggtggtgat
     3241 atccagcggc gacaaggacc tggcgcaact ggtcacgccg cacatcgtga tcgtcgacac
     3301 catgaccggc aagcgccgcg acatggccgg tgtgcaggcc gagttcggcg tgcgtcccga
     3361 ccagatgctg gactaccagg tgctgctggg cgacgccatc gacaacgtgc cgggggtgga
     3421 caaggtcggc cccaagaccg ccgcccgctg gctgggcgaa tacggctcgc tcgacgccat
     3481 cgtggcgcag gccgaccgca tcaagggtgc ggccggcgac aacctgcgcc gcgcgctggc
     3541 ctggctgccg accgggcgcc ggttgctgca gatccgcacc gactgcgatc tgacaggcca
     3601 tgtcgatggc ctgcctgctc tggattcaat agctgctggc gcaagcgaca caagcgcgct
     3661 ggccgagttt tatgacaaat acgggttccg tacgctggct cgcgcgctgc gccccgccgc
     3721 agccggcgac gccgccccga acgccgcgcc ggtgccggcc gcggctgcga cgggcgggca
     3781 cgacgtggcg tacgaaacca tcctggactg gccggccttc gagcgctggc tggcgcgcat
     3841 cgaagcggcc gaactgaccg cgctggacac cgaaaccacc tcgatcgacg agcagcaggc
     3901 gcgcatcgtc ggtctttcgc tcagcgtgcg gccgcgtgag gccgcctaca tcccgctggc
     3961 gcacgacggg cccggcgccc ccgcgcagct gccgctgggc gaggtgctgg cgcgcctgaa
     4021 gccgtggctg gaaaacccgg cccgcaccaa gctcggccag cacgtcaagt acgaccgcca
     4081 tgtgctggcc aaccacggca tcgaggtggc cggttacgtg cacgacacca tgctgcagag
     4141 ctatgtgctc gaagtgcatc gcccgcatgg cctggccagc ctggccgaac gccacctggg
     4201 gcgccgcggc atcgactacg aagcgctgtg cggcaagggc gtgcaccaga taccgttcgc
     4261 ccaggtgccg atcgacaagg ccgcccacta cgcctgcgag gacgccgatc agacgctgga
     4321 agtgcatctg gcgctgtggc cccaggtgca ggccgagccg gggctggcgc gcatctatgc
     4381 gctggagatc gccaccagcg aagtgctcta ccgcatcgag cgccacggcg tgctgatcga
     4441 cgctgcgcag ctgcagcgcc agagccacga gctgggccgc cgcattctgg agctggagcg
     4501 ccaggcgcac gagctggcgg gccagccctt caacctcggc tcgcccaagc agatcggcga
     4561 gatcttcttc gacaagctgg gtctgccggt ggtcaagaag accgccaccg gcaagcccag
     4621 caccgacgag gaggtgctgg aaaaactggc cgaagactac ccgctgccgg cgcgcatcct
     4681 ggagcaccgc ggcctggcca agctcaagag cacctacacc gacaagctgc cgcagctggt
     4741 ggacccggtc agcggccgcg tgcacaccca ctatgcgcag gcggtggcgg tgaccgggcg
     4801 cctggccagc aacgacccca acctgcagaa catcccggtg cgcacgcccg agggccggcg
     4861 catccgcgaa gccttcatcg cgccccccgg ccatgtcgtc gtcagctgcg actacagcca
     4921 gatcgaactg cgcatcatgg cgcacctgag cgacgatgcg gcgctgctgg ccgccttcca
     4981 cgcgggcatg gacgtgcacc gtgccaccgc cgccgaggtg ttcggcgtcg cgcccgagca
     5041 ggtcagcagc gagcagcgcc gttatgccaa ggtgatcaac ttcggtctca tctacggcat
     5101 gagcagcttc gggctggcgc gcaacctcgg catcgacaac acggcggcgc gcaactacat
     5161 cgaccgctat ttcgagcgtt acccgggcgt gcggcgctac atggacgaca cgcgccaggc
     5221 ggccaaggcg cgcggcttcg tcgaaaccgt gttcggccgc cggctgtacc tgcccgagat
     5281 caacagcccc aacgggccgc gccgggccgc cgccgagcgc gccgccatca acgctcccat
     5341 gcagggcacg gcggccgacc tgatcaagat gagcatggtg gcggtgcagg ctgccatcga
     5401 ccagcagcgg cgcgccagcc gcatgatcat gcaggtgcat gacgagctgg tgctggaagt
     5461 gcccgaggcc gagctcgcgt gggcgcgcgc cgaggtgccg cgcctgatgg ccggtgtggc
     5521 ccagctcaag gtgccgctgc tggccgaggt cggcatgggg ccgaactggg agcaggcgca
     5581 ctgagggtgt tctgatcgct atcagcctcc agcgcccatc cagcaagcgc cgacagctat
     5641 gattttgaga gcgtctcggc tcagctggcg gcgtcggcgt cgaggtgata gcgggtcacg
     5701 cgctcgactt cgtttttgct gcccaggatc acgctgacgc gttcgtgcag ttgctgcggc
     5761 tgcacgtcca ggatgcgctg gcggccgttg gtggcggcgc cgccggcctg ctcgatcagc
     5821 cagctcatcg ggttggcctc gtacatcagg cgcagcttgc cgggcttgtt gggttcgcgc
     5881 ttgtcccagg ggtacatgaa gacgccgccg cgcatcatga tgcggtgcac gtcggccacc
     5941 atgctggcga cccagcgcat gttgaagttc ttgccgcgtg gcccgtcctt gccggccagg
     6001 cattcgtcga tgtagcggcg caccggcgcg tcccagtggc gcaggttgct catgttgatg
     6061 gcgaactcct gcgtatcgtc gggaatgcgc aggtcgtcct gggtcagcac gaagctgccc
     6121 agttcgcggt ccagggtgaa catggccacg ccgtcgccca ccgtcagcac cagggtggtc
     6181 tgcggcccat agacgcagta accggccgcc acctggtggc tgccgggttg caggaagtct
     6241 tcttcctcga cgccgcggtc gccttcgggc atcttcagga cgctgaagat ggtgccgatg
     6301 ctgacgttga cgtcgatgtt gctggagccg tccagcgggt cgaacagcag caggtattcg
     6361 cccttcggat aacggttggg caccacgtgg atggcttcca tctcctcgct ggccatggcc
     6421 gccaggtggc cgccccattc gttggcgtcg atcagcacct cgttggcgat gatgtccagc
     6481 tttttctgca cctcgccctg cacgttctgg ctgccggcgc tgcccagcac gccgcccagg
     6541 gcgcccttgt tgacggcgtg gctgatgctc ttgcaggcgc gtgccaccac ttcgagcagc
     6601 aggcgcagct gggccggaat gtggccggcg atgcgctgct tttcgaccag gtagcgcgac
     6661 agggagatgg gcgagctcat gcggatcctt tctgctgcgt gtcgggggtt tcggtggtgc
     6721 gcgcctcggc gtcggcggcc agcgccggcg cctggccggc gagggcgcgc gtgacgactt
     6781 cacgcacgtc gttgctcagg tcgggcctgg cggcgacgcg ggcgatggcc tcgcgggcgg
     6841 cactgcggta tggctcggcc agacgtgccc aggcgtccat ggcacgcgcc agccgcgccg
     6901 ccacctgcgg gttgagggta tcgagctcca gcacgcggtc ggcccagaac aggtagccgg
     6961 cggcgtcgcg ccggtgaaag ccggccgggt tgttgtggca gtaggcaaag atcaggctgc
     7021 gcgcgcggtt cgggttgcgc agactgaagt cggggtgctt catcagcagc cgcaccgtcg
     7081 gcagcgctcc gccgcggcgg tcgggcgcac gcgcctgcag ggcgaaccat ttgtcgagcg
     7141 ccagcggctc atggcggaac aggcggtgaa agcgcgacag ggcatgcgcg cccagctcgt
     7201 gcccgcagcc gaccagggcg cccagcgcgt tcaggcggtc ggtcatgttg ccggcgtcct
     7261 tgacgcgctg gtaggccttg ccgggccaga cgctgtcgcc ctgttcgctg gcggccagac
     7321 acagcatggc cagcgccagc ccggccagcg cgcgccggcc gctggagtgc gggtcggggc
     7381 ggtaggcgcc ggtgtcgtgg tgttgctccc aggcccattg ccagtcgtcg cgcaaggcgg
     7441 tggccagctg ccgctgcatg gcgtcgcgca cggcgtggat gcgctgcgga tcgaccacgt
     7501 cgagctgatc ggcgatgtag tcctcgtcgg gcagcttcag caccagggct ttgaaggcgg
     7561 ggtccagctg gggctggcgc agcaccgcgc gcagggcctg aagataattg tctttcaggg
     7621 gtctggcgct tacctggtct gcaccggaag ctatgttttc aagagcaatg cggacgccca
     7681 ggcgctggcc ggcgtcccag cggttgaagg ggtcgctgtc gtacgccagc aggtgcagca
     7741 gctgctcgtc ggcgtccagt ccgcgcagtt cgaccggcgc cgagaagccg cgcagcagcg
     7801 acggcacggg cggctgatcg acctcgctga agaccagggt ggtctgcggc tcggtcagca
     7861 cgcaggtgtc gccgtgcagc agctcgcccc ggctcagcgt cagcggcagc tcgcgcccgt
     7921 cggcgcccac caggccgagc cgcaccggaa tcacgaacgg cgccttgtgc ggctggccgg
     7981 gcgtggccgg gcagctttgc gacagcgtca gggtgtagcg gcgcgcctgg gcgtcgtact
     8041 ggccggtcgc ctgcagcacc ggcgtgccgg cctggctgta ccagcgcttg aactgcggca
     8101 gcagaggcgc cagtgtgctg cccgggttgg cgtcggccat ggcctgggcg aagtcgtcgc
     8161 aggtcacggc ctggccgtcg tggcgctgga agtacaggtc catgccgcgg cgaaagccgg
     8221 cacggcccaa caggctttga atcatgcgca ccacctcggc gcctttttcg tagatggtgg
     8281 tggtgtagaa gttgctgatt tccaggtatt cgtcgggccg caccgggtgg gccatggggc
     8341 tggcgtcctc ggggaactgc gcggcgcgca gggcgatgac gttttcgatg cgtttgacgg
     8401 cgcgtgcgct gggggtgccg gccaggtcct cgctgaattc ctgatcgcgg aacacggtca
     8461 ggccttcctt gagcgagagc tggaaccagt cgcggcaggt gacgcggttg ccggtccagt
     8521 tgtggaagta ctcgtggccg atgacgctct cgacggcgtc gtagtcgccg tcggtggcgg
     8581 tggacggctt ggccagcacg tacttggtgt tgaagatgtt caggcccttg ttttccatgg
     8641 cgccggcgtt gaagtcgctg gtggccacca ccatgaagcg ctccaggtcc agcggcaggc
     8701 cgaagcgcgc ctcgtcccac gccacggcgt ggatcagcga gcgcatggcg tgttcggtct
     8761 tgtccaggtc gcccgggcgc acgaagacct gcagcaggtg ctcgctgccg gtgcgtgtgg
     8821 tgatgcgctg ctcgcgcgcc accaggttgc cggccaccag ggcgaacagg tagcagggct
     8881 tgcggtgcgg gtcgtgccag gtggcgaagt ggcgcccatc gtccagctcg ccggcgtcca
     8941 ccaggttgcc gttggccagc agcaccggcc agcgcgcctt gtcggcgcgc agggtgacgg
     9001 tgtaggtggc catcacgtcg ggccggtcga ggaagtaggt gatgcggcga aagccttccg
     9061 cttcgcactg ggtgaagaag tcgccgccgc tcatgaacag gcccatcagc ttgctgttct
     9121 tctcgggcgc gcaggtggtg aagatttcca gctcgaacgg cgcgtggtcg gccggcagct
     9181 gctccagtac cagggtctgg ccgtccaggc ggaacgaaca ggcctgaccg cccagcatca
     9241 cgcgcgagag gttgagctcg tcgccgtcca ggcgcagcgg ctgcgccgcc acgtccgggt
     9301 tgcggcgcag ccgcaggcgg ctcagcaccc gggtcttggc cgggtccaga tcgatggtca
     9361 gctcgacgct gtcgatccag aaggcgggcg gggcgtagtc ctggcgccgg gtcacggcgg
     9421 tttgtccttc acgcatggcg gatcctgtgt tgctctgatt gtgatagctg ctcgcgctct
     9481 cccagtctgc gcgagaggcc ctaaaggctt gcaaatgggc ttacacgccc tgtttcaggc
     9541 tggcctcgat gaaggggtcg aggtcgccgt ccagcacttt ctgcgtggcg ctgatttcga
     9601 cgccggtgcg cagatccttg atgcggctgt tgtccagcac gtagctgcgg atctgatggc
     9661 cccagccgac gtcggtcttg ctgtcttcca gcttctgctg gtcttccatg cgcttgcgca
     9721 tttcgtggtc gtacaggcgc gcgcgcaggc gctgccaggc gacgtcgcgg ttgctgtgct
     9781 ggctgcggct gtcctggcac tgcaccacga tgccggtggg gatgtgcgtc aggcgcacgg
     9841 ccgagtcggt cttgttgatg tgctggccgc cggcgccgct ggcgcggtag gtgtcggtgc
     9901 gcacgtcggc cgggttgatg tcgatctcga tcgaatcgtc cacttcgggg tagacgaaca
     9961 cgctggcaaa gctggtgtgg cgcccgcccg aggaatcaaa gggcgacttg cgcaccaggc
    10021 ggtgcacgcc ggtttcggtg cgcagcaggc cgtaggcgta ttcgccctcc accttgatgg
    10081 tggcgctctt gatgccggcg ctgtcgcccg gggtttcttc ctggatttcg gtgttgaaat
    10141 ccttgcgctc ggcgtatttc aggtactggc gcagcagcat gctggcccag tcgcaggcct
    10201 cggtgccgcc ggcgccggcc tggatgtcga tgaagcagtt gttggggtcg gccggctggt
    10261 tgaacatgcg gcggaattcg agctcctcca ccgtccgggc cagcttttcg acgtcgctgc
    10321 cgatggcctg caggctggcg tcgtcgtttt cctcgcggct gagttcgaac agctcggcgc
    10381 tgtcggtcag gccctgggtc agctcatcga gcgtgaccac cacgccatcg agctgctttt
    10441 tttccttgcc cagctcctgg gccttcctgg ggtcgttcca gaccgccggg tcttccagtg
    10501 cggcgttgac ggttctcagg cgttcggcct tgccggcgta gtcaaagata cccccgtagc
    10561 gcgtcggtgc gcgcacgcag gtcgtcgatt tgggcgctga tctggttgag gcgttctgct
    10621 tccatgggtt gctgtcctgt gctgtggctg gctgcgcgcc cggtggctgg ggcgcgaggc
    10681 gtggtgtgtg acctggggtt gaacgcaagg cgccggggcc acctggcccg gcggcatgcg
    10741 ccgatcgggg gtggggcagg ccgtcgtcaa cccgtgaggg cggcattttc tcacgccggg
    10801 cgggtgatcg gccgcccgcc gcgcccgccg gcgttacggc ggtgacagaa agtcctgcag
    10861 cgcggcggcc aggcgctcgg gctggtcgtg gtgcagcatg tggccgctgt cgggcagctg
    10921 ctgccggcgc agatccggca ccgcctgcag ccgctgatgg aattccgcca gcgtgtagcg
    10981 ccccttccac cactgcgaca ggctgtcgtc gctgccctcg atcaccagca ccggcgcggt
    11041 gatggccgcg tacagcgcca gcatctcgtc gacccggaac aggctggggt tgacgatctt
    11101 gtgcgccgcg tcgcccagga tccgccactg gccgtcggcc cctggtcgcg cccactggcg
    11161 cgccagccag gcggccttgt cggccggcag gcgcgggttg gtcttcatca ggcgctgggc
    11221 cacggcgtcg gcgtcggggt aggtcttgag gctgatttcg cctcggcgca gctgttgcag
    11281 ctcgtccagc cattgcgcca gccggcgcgg cgcctgcgtc ggccggctgg ccggcatgcc
    11341 gaagccttcc agattgacca gtcggcggat gcgcgccggc cgcgccccgg cgtactgcat
    11401 caccacgttg ccgcccatgc tgtggccgac caggtcgacc ggctggccgt tcggggcgta
    11461 gtggtcgagc agggcgtcca gatcgcccag gtagtcggcc agcacgtagt ggtcgcaggg
    11521 ggcggccagc gtggtgtcgc caaaaccgcg ccagtcgggc gcgatgatca ggcgctggtc
    11581 ggcaaactcg gcgctcagcg cgtcgaccat gaactggtag gacgccccca cgtccatcca
    11641 gccgtgcacc agcaccagcg gcgtgctgcc cggctggggt cggccccagc agttgacgtg
    11701 gacatggatc tggcgcaggg gcagcgattc gctgcgcgcg ggacggtgcg gcacgtaaga
    11761 catgggcggc gattatttcc cggccgcccg cagcgtgccg gtctgggggt taacccgctc
    11821 agacatcgcg cgcttgtcac gtaaaaaccc ggcgtggcgg gttggccggt gccgagcgga
    11881 cggcgccgcg acccggcaat cgttgtcgag aggagatgaa acatgaaaca caagcttctg
    11941 gtgatggccc tgggcgcggc gctggcggcg ccggccgcga tcgccggcac ggtgacggtg
    12001 ctgacctcgt tccccaagga gctgaccacc gcctacaaga aggcgttcga gtcggcccac
    12061 ccggggatca cggtggaaat cctgaacaag aacaccaccg cgtcggtcgc ctacatccgc
    12121 gagctgcccg agggccagcg ccccgacgtc atgtgggcca gcgcgcccga cgccttcgag
    12181 gtgctgtcca gcttcaagct gctgcaaagc gcgcccgagg tggtgaacaa gagcgcgccg
    12241 gccaagatcg gcaactaccc gctcaacgac cccaagggca tgtactacgg ccaggcgctg
    12301 gcgggctacg gcatttcgtg gaacacgcgc tacctcaagg cgcacaagct cacggcgccg
    12361 aaggaatgga gcgacctggt gcgtcccgag tacttcggcc atatcgccat ctcgtcgccc
    12421 tcgcgttcgg gcacgaccca gctcacggtg gaaaccatct tgcagggtga gggctgggac
    12481 aagggctgga
//
LOCUS       DAOOOG010000065        12200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_5768_length_12200_cov_28.812515, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000065 DAOOOG010000000
VERSION     DAOOOG010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5768_length_12200_cov_28.812515"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PK242_06555"
     CDS             complement(<1..541)
                     /locus_tag="PK242_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOZ93715.1"
                     /translation="MATRNTHAFASTRKRFQTASGRSGELYSLPALARRFPNVKRLPV
                     SLRLVLESVLRHCDGVKVTPEHVEQLANWRPKAARTDEIPFTVARVILQDFTGVPLLA
                     DLAAMRSTAQRLGQDPKKVEPLVPVDLVVDHSVMIDFYGKHNALDLNMKLEFQRNRER
                     YEFMKWGMQAFDTFGVVPPG"
     gene            complement(728..1345)
                     /locus_tag="PK242_06560"
     CDS             complement(728..1345)
                     /locus_tag="PK242_06560"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HOZ93716.1"
                     /translation="MNKKLAALLALTLLGTAHAQSGLKAGLWENRTVRMMVDGKDMTA
                     AMRDAAELRKQQIAKLPPAQRQQAEAALARQGGDPLSRRVCISAEMAARQQPMVPRPP
                     RAECEPPKFKRDGQRTTFELSCTQEGGKLSGKGEIVTTADLITTRLETRSSDGAGQAH
                     TLLAETQMKYLGSDCGGIKPLEAPAQPASAAAATSRSPRKPAPAK"
     gene            complement(1494..2003)
                     /locus_tag="PK242_06565"
     CDS             complement(1494..2003)
                     /locus_tag="PK242_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOZ93717.1"
                     /translation="MIREVEGDILLSQAQVIAHGIATHDPFDSGLALALRERFPSMVK
                     DYRHATHAKAPDTGEIWAWRGVNEDGSTRGIVNLLTQGMQSQDKAARPVKARLEDVNR
                     ALRALARHLQDEGVKSVALPRLATGVGALDWADVKPLIQQHLGGLGIPVLVYEVYHKG
                     MKADEKLAA"
     gene            2141..3550
                     /gene="yegQ"
                     /locus_tag="PK242_06570"
     CDS             2141..3550
                     /gene="yegQ"
                     /locus_tag="PK242_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HOZ93718.1"
                     /translation="MKAPELLLPAGSLDKMRAAYDFGADAVYAGQPRYSLRARNNEFR
                     LEQLAQGIAEAHARGKKFFVTSNLIAHNDKLRTYLRDLQPVIDLRPDALIMADAGLIM
                     QVREKWPHIPIHLSVQANTTNWAAVKFWQSVGVARIILSRELSLAEVEQIRQECPDME
                     LEVFVHGALCIAYSGRCLLSGYFNRRDPNQGTCTNACRWNYTTQAADVDPNTGEALAR
                     GQQLTGFNFANEQQRADQDGGARAARHPAADKVWLIEEEGRPGQLMPIMEDEHGTYIM
                     NSKDLRAVELVEKLAKIGVDSLKIEGRTKSLYYVARTAQVYRRAIDDAVAGRPFNPQL
                     ITELEGLANRGYTSGFLERRPAQDYQNYETGHSVAQRSQFVGEVKAVQDGWAEVETKN
                     RFAVGDWIEIIHPQGNRTVQLQHMKNADGQPIDVAPGNPLRVWIPVDGPAEQALIARL
                     MAPPQPAPAAASAPQGVTI"
     gene            3599..4324
                     /locus_tag="PK242_06575"
     CDS             3599..4324
                     /locus_tag="PK242_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOZ93719.1"
                     /translation="MSRLCPQCGAENRDAAKFCLKCAHQLVPLSEPTQPLPLEQPARK
                     RRRRKRPAEAPPRKRHAWLWGLVALVAVVALGGALWRGDTRPDTAPPQAAATPAGAAA
                     SSSPAAVEPASPASAASAAPPPLPAASSAPIPSTSSAAPAAPTRPSTAARRSKPSPPA
                     AEPAVVAPAEVAPPAAAAPPEPVASAPARPAPPRELCADTRFLNRAVCLQNECSKPGL
                     RQHPQCVQMREQQDALRRGAGDH"
     gene            4775..7345
                     /gene="mutS"
                     /locus_tag="PK242_06580"
     CDS             4775..7345
                     /gene="mutS"
                     /locus_tag="PK242_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009931111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOZ93720.1"
                     /translation="MMAQYLRLKADFPDTLLFYRMGDFYEMFYADAERAAGLLDITLT
                     TRGQSAGAPIPMAGVPFHSVDGYLARLIKLGESVAICEQVGDVATAKGPVERKVVRVV
                     TPGTLTDAELMSDKNESLLLAVHPGARGRIGLAWLAVTQGELHLAECASGELPGWIER
                     IGASELLLSQDAPPALQQALSQGPRAALTRRPDFQFDAPLGRRKLLEQLQAASLAGWD
                     AEELPHAHAACAALLSYAEHTQGRALTHVRALVVERAGTLIELPASTRRNLELVQTLR
                     GEDAPTLLSLLDTCLTGMGSRRLKSWLLAPARERAEASARLAAIGALRAGGLWQKLRA
                     HLKGSSDVERITARLALGQVRPRELVALRQTLQKTELLALVQQAPEPLLMQIAADLAP
                     PAEALDLLQAALLPEPAALIRDGGVIAPGLDQELDELRAISDNCDAFLLELEARERAR
                     TGIANLRVQFNKVHGFYIEVTQGQAAKVPDDYRRRQTLKNAERFITPELKAFEDKALS
                     AQERALAREKWLYEHLLNQLQPHVPQLTRLGRAIAALDALAALAERSLTLNWCAPEFT
                     AHPCIEIRAGRHPVVQARLQETSGQPFIANDTLLGPKTRMQVITGPNMGGKSTYMRQV
                     ALIALLASVGSYVPAQSCRLGPLDAIHTRIGAADDLANAQSTFMLEMTEAAQILHGAT
                     AQSLVLMDEIGRGTSTFDGLALASGIAAHLHDKVQAFTLFATHYFELTEFPATHHAAV
                     NVHVAAAETAGSGGHDIVFLHELQPGPASRSYGIQVARLAGVPAAVVHHARHALAQLE
                     QGASAAQVQVDLFAPPPPAAEPAPSPLDAALAALDPDQLSPREALDALYRLKKLARAG
                     "
     gene            complement(7584..7895)
                     /locus_tag="PK242_06585"
     CDS             complement(7584..7895)
                     /locus_tag="PK242_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93721.1"
                     /translation="MSTPTPPSLPPELIDALRQRNLPQLLKVLLSQRPAASSPPAAPA
                     RPPAARPATGAGKRPPEAELPQVAAEGLSPGEVPRTGTTFWGWLMAALLAYLAYRLWG
                     G"
     gene            8025..8864
                     /locus_tag="PK242_06590"
     CDS             8025..8864
                     /locus_tag="PK242_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ93722.1"
                     /translation="MSTIVFSHGNSFPASTYRVMLDSLRARGFEVHAIEKYGHDPKYP
                     VTDNWPHLVRQLAQFATQRSKDDHGGAPWLVGHSLGGLLSLMCAARHPELARGVVMID
                     SPVLSGWRAGAVRVVKRTPLMRRLSPSSVARRRRKEWDDRQAVYEHFRAKKVFAAWDP
                     RVLRDYVDHGTLDADGSWRLAFDPEVESRIYETVPHNLGPLLRRHPLRCPVAFVGGIS
                     SVEMRQVGGLDATAEVAQGRVTMLEGSHLIPMEQPLAVAAAIEAHIRAMPTAATAAPG
                     TPG"
     gene            complement(8827..9411)
                     /locus_tag="PK242_06595"
     CDS             complement(8827..9411)
                     /locus_tag="PK242_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HOZ93723.1"
                     /translation="MHQQCSLFADEAVTLVHDAEGGIVYHPACVPQASGALWLAALLA
                     QTPWRRLQRPMYERVVEVPRLVASVALRDPARPPCIDAALAQVQAVAPAPYTHVGLNL
                     YRDGRDSVAPHGDRQAELEPGWPIAIVSLGAPRDMLIRALAGGASWRITLEPGSLLRM
                     SHASQRTHLHAVPKCAQALGPRISLAFRVRRSPP"
     gene            9574..10254
                     /gene="tenA"
                     /locus_tag="PK242_06600"
     CDS             9574..10254
                     /gene="tenA"
                     /locus_tag="PK242_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HOZ93724.1"
                     /translation="MNPASTTTFSQAAWQGNLALYHDTLALPFNQELAAGTLSVERFH
                     HYIIQDAHYLVAYGRALAVAAAKADDADGLMQFTEAAKVAVLVERQMHGGFMQRFGVT
                     TEQFAATPLTPACHHYTQFLLATAWAEPYPVVLASLLPCFWIYAEVGRAIHASARQPN
                     PYQDWIDTYAGEEFHTAVRGVLATIDRVADGVDARTLQAMHAAYTMSARLEWMFWDSA
                     HRLGRWPV"
     gene            complement(10390..10938)
                     /locus_tag="PK242_06605"
     CDS             complement(10390..10938)
                     /locus_tag="PK242_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOZ93725.1"
                     /translation="MRLWLLRHAEVAAPPGLCYGASDLPADAAATRAAARALAERLPH
                     GLCLQVSPLQRCELLAQSLQALRPDLTLKFDARLREFDFGTWEGRPWGDIARAEFDAW
                     LADFALARPGGGECVAELMARVALAWDDWRASGREAAWVTHAGVIRAARLVARGQRQV
                     GAASDWPAEGLAFGASLVLQAG"
     gene            complement(10935..11729)
                     /locus_tag="PK242_06610"
     CDS             complement(10935..11729)
                     /locus_tag="PK242_06610"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOZ93726.1"
                     /translation="MAALRHYLLALQFFTRIPVTGRLAAWVGYSPAQLNASAGHFPGV
                     GWVVGAVGAAVLWLAALRLPAGALGALVAAGLSTAATAWLTGAFHEDGLADTCDALGG
                     QVSRERALEIMKDSRIGSYGTLALVLALGLKLALLALLLQRGVAGAAAALLAAHVLSR
                     LAPLAVMRVLPYITEGPQAKSKPLADAVSGTSVVTSVLWSIPALALMGWSQGATETTV
                     AMALWALTLMAVLRWLRRRLQGFTGDTLGATQQLCELALYLGLALA"
     gene            complement(11737..>12200)
                     /locus_tag="PK242_06615"
     CDS             complement(11737..>12200)
                     /locus_tag="PK242_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93727.1"
                     /translation="EISGCCIQAAPRDYARFGQFILEGAQVDGQPAAGPLPRPAAAPS
                     GGSATGEAVERGGASILPEGYLQQATHKQADIGAPGRGYGYQWWTYDDGSFAARGIFG
                     QGIFIDPARELVIVTNGDWAKAGSANNPQYAAREAFYQTVRRAIDDEAAGR"
BASE COUNT         1820 a         4501 c         4190 g         1689 t
ORIGIN      
        1 agcccggcgg caccacgccg aaggtgtcga aggcctgcat gccccacttc atgaactcgt
       61 agcgctcgcg gttgcgctgg aattcgagct tcatgttcag gtcgagcgca ttgtgcttgc
      121 cgtagaagtc gatcatcacc gagtggtcga ccaccaggtc caccggcacc agcggctcga
      181 ccttcttcgg gtcctggccc aggcgctgcg cggtggagcg catggccgcc aggtcggcca
      241 gcaggggcac gccggtgaag tcctgcagga tcacgcgggc gacggtgaac gggatctcgt
      301 cggtgcgcgc cgccttgggg cgccagttgg ccagttgctc gacgtgctcg ggcgtcacct
      361 tgacgccgtc gcagtggcgc agcaccgact ccagcaccag ccgcagcgac accggcaggc
      421 gcttgacgtt ggggaagcgc cgggccagcg ccggcagcga atacagctcg ccgctcctgc
      481 ccgacgcggt ctgaaagcgc ttgcgggtgc tggcgaaggc atgggtgttg cgcgtggcca
      541 tggcgtgtgc tcctgggtcg atgggcgatg gcggcgccgc gccgcccaag gtcattgtcg
      601 caggcccagg cggggccggc cagcacccgg ggccacaacc ctgattccaa agccgaaact
      661 cgatctgcgc ttgtctggca agcgccgaca gctacgtttt cagtagcatt tcagccctgg
      721 ccgggtgcta cttcgcgggc gccggcttgc gtggcgaacg gctggtcgcg gcggcggccg
      781 aggccggttg cgccggcgct tcgagcggct tgatcccgcc gcagtcgctg cccaggtact
      841 tcatctgcgt ttcggccagc agggtgtggg cctggccggc gccgtcgctg ctgcgggttt
      901 cgaggcgggt ggtgatcaga tcggcggtcg tcacgatctc gcccttgccg ctcagcttgc
      961 cgccttcctg ggtgcagctg agttcgaagg tggtgcgctg gccgtcgcgc ttgaacttcg
     1021 gcggctcgca ctcggcgcgc ggcgggcgcg gcaccatggg ttgctggcgc gccgccatct
     1081 cggcgctgat gcacacgcgc cgcgacagcg ggtcgccgcc ctggcgcgcc agcgccgcct
     1141 cggcctgctg gcgctgcgcg ggcggcagct tggcgatctg ctgcttgcgc agctcggccg
     1201 cgtcacgcat ggccgccgtc atgtccttgc cgtccaccat catgcggacg gtgcggtttt
     1261 cccacaggcc cgccttcagg cccgattgcg catgggcggt gcccagcagg gtgagcgcca
     1321 gcagcgcggc aagcttcttg ttcatgagcg atcgaccttg caaaggatgc gtggggcgcc
     1381 gatggtacaa aaaaagcgcc cgcaccgaag ccggtgcggg cgctggtgcg ccgaccccgg
     1441 cgccgcgcgt ggcccgggcg ggagcgtcag aacgcaggcc tgaggggctc gtctcaggcg
     1501 gcgagctttt cgtcggcctt catgcccttg tgatagacct cgtacaccag caccggaatg
     1561 cccaggccgc ccaggtgctg ctggatcagc ggcttgacgt cggcccagtc gagcgcgccg
     1621 acaccggtgg ccaggcgcgg cagcgccacg ctcttgacgc cttcgtcctg caggtgcctg
     1681 gccagggcgc gcagcgcgcg attgacgtct tccagcctgg ccttgaccgg gcgcgcggcc
     1741 ttgtcctggc tttgcatgcc ctgggtcagc aggttgacga tgccgcgcgt ggagccgtct
     1801 tcgttgacgc cgcgccaggc ccagatctcg ccggtgtcgg gcgccttggc atgggtggcg
     1861 tggcgatagt ccttgaccat cgacgggaag cgctcgcgca gagccagcgc caggccggaa
     1921 tcgaacgggt cgtgggtggc gatgccgtgc gcaatcacct gagcctggct cagcagaatg
     1981 tcgccttcga cttcacggat catggttttc tccttgggac gggttacgaa tctctcgatg
     2041 ctttgacctt agcccagtca gggttgatgg ttcttgactg ccgtcaagcc gatgcccttt
     2101 ggcgcccctg cccggccccc tgcgccgaca ataggccccc atgaaagccc ccgaactgct
     2161 gctgcccgcc ggcagcctgg acaagatgcg cgccgcctac gactttggcg ccgatgccgt
     2221 gtacgccggc cagccgcgct acagcctgcg tgcgcgcaac aacgaatttc gcctggagca
     2281 gctggcccag ggcattgccg aggcgcacgc gcgcggcaag aaattcttcg tcaccagcaa
     2341 cctgatcgcg cacaacgaca agctgcgcac ctacctgcgc gatctgcagc cggtgatcga
     2401 cctgcggcca gacgccctga tcatggccga cgccgggctg atcatgcagg tgcgcgaaaa
     2461 gtggccgcac atcccgatcc atctgtcggt gcaggccaac accaccaact gggcggcggt
     2521 gaagttctgg cagtcggtgg gcgtggcgcg catcatcctg tcgcgcgagc tgtcgctggc
     2581 cgaggtcgag cagatccgcc aggagtgccc cgacatggag ctggaggtgt tcgtgcacgg
     2641 cgcgctgtgc atcgcctaca gcggccgctg cctgctgtcg ggctacttca accggcgcga
     2701 ccccaaccag ggcacctgca ccaacgcctg ccgctggaac tacacgaccc aggccgccga
     2761 cgtcgatccc aacaccggcg aggcgctggc gcgcggccag caactgaccg gcttcaactt
     2821 cgccaacgag cagcagcgcg ccgatcagga cggcggcgcc agagccgcac gccacccggc
     2881 cgccgacaag gtctggctga tcgaggaaga aggccgcccc ggccagctga tgcccatcat
     2941 ggaagacgag cacggcacct acatcatgaa cagcaaggac ctgcgcgcgg tcgagctggt
     3001 ggaaaagctg gcgaaaatcg gcgtcgactc gctcaagatc gagggccgca ccaagagcct
     3061 gtactacgtg gcgcgcaccg cccaggtgta ccgccgcgcc atcgacgacg cggtggctgg
     3121 ccgccccttc aacccgcagc tcatcaccga gctggaaggc ctggccaacc gcggctacac
     3181 cagcggcttt ctggagcgcc gcccggcgca ggactaccag aactacgaga ccggccattc
     3241 ggtggcccag cgcagccagt tcgtcggcga agtgaaggcc gtgcaggacg gctgggccga
     3301 ggtggagacc aagaaccgct ttgccgtcgg cgactggatc gaaatcatcc acccgcaggg
     3361 caaccgcacc gtgcagttgc agcacatgaa gaacgccgac ggccaaccca tcgacgtggc
     3421 gcccggcaac ccgctgcgcg tgtggattcc ggtcgatggg ccggccgagc aggcgctgat
     3481 cgcgcgcctg atggcgccgc ctcagccggc gcctgcggca gcgtcggcgc cgcaaggtgt
     3541 taccatctga ttcatacttt caggttaatc gctgcgcccc ggaccgccca gccctcgcat
     3601 gtcccgcctc tgcccccaat gcggcgcgga aaaccgcgat gccgccaagt tctgcctgaa
     3661 gtgcgcgcat cagctggtac ccctgagcga gccgacccag ccgctgccgc tggagcagcc
     3721 ggcgcgcaag cgccggcgcc gcaagcgccc ggccgaggcg ccgccgcgca aacgccacgc
     3781 ctggctgtgg ggcctggtgg cgctggtggc cgtggtcgcc ctgggcggcg ccctgtggcg
     3841 cggcgacaca cgtccggaca cggcgccgcc gcaggccgcc gccacgcctg cgggagccgc
     3901 cgcttcctcg tcgccagccg cggtcgagcc ggcgtcgcca gccagcgcgg ccagcgctgc
     3961 accgccgccc ctgccggcgg catccagcgc gccgatccca agcacctcca gcgcggcccc
     4021 ggcggcgccg acgcgtccat ccaccgccgc gcggcgcagc aagccatcgc cgccggccgc
     4081 cgagccggcc gtcgtcgccc cggccgaggt ggcgccgcct gccgcagctg cgccacccga
     4141 gccggtggcc agcgcgcccg cccgcccggc gccgccgcgc gagctgtgcg ccgacacacg
     4201 ctttctgaac cgcgccgtct gcctgcagaa cgagtgcagc aagcccggtc tgcgccagca
     4261 cccgcagtgc gtgcagatgc gcgagcagca ggacgccctg cgccggggcg ccggtgacca
     4321 ctaaaccttg tccactgaaa cactgaaatc ggttcggcgc tgtccgcccg atacgggcac
     4381 gctgcggcgt tgcccatgct cgccacagca cgggctgtgg ctgcgctttg cgccttgcat
     4441 cgcctcccgt ctcgggtgtc cctcgccgcc catttcaagg tttcagtgga cccgtgctga
     4501 acgtcccctg gcacgcccct gacaccggct gcgccggcag cctgggcgcc cctcccgcca
     4561 tcgaagcgca gcgaggtcat tcgtcgttct tcatccgccc ggcgtgaagc cggtcggcac
     4621 gcaccacacg ccaacccgcg caaggggccg tgccggcgac gcgcccgagc gcgccgctac
     4681 catgctgccc atgccccgac cgaccgcgca ggccgctgcg ccccaacccc acaccgcgcg
     4741 cccgggtgaa ccgcccgaag gccagcacac ccccatgatg gcgcagtacc tgcgcctgaa
     4801 agccgacttt cccgacaccc tgctgttcta ccgcatgggt gatttctacg agatgttcta
     4861 cgccgacgcc gagcgcgccg ccggcctgct ggacatcacg ctcaccacgc gcgggcagtc
     4921 ggccggcgcg cccattccga tggcgggcgt gccgtttcat tcggtcgacg gctacctggc
     4981 gcggctgatc aagctgggcg agtcggtggc gatctgcgag caggtgggcg acgtggccac
     5041 ggccaagggg ccggtcgagc gcaaggtggt gcgcgtggtc acgcccggca cgctgaccga
     5101 cgccgagctg atgagcgaca agaacgaatc gctgctgctg gccgtccacc ccggcgcgcg
     5161 cgggcgcatc ggcctggctt ggctggcggt gacgcagggc gagctgcacc tggccgaatg
     5221 cgccagcggc gagctgccgg gctggatcga gcgcatcggc gccagcgaac tgctgctgtc
     5281 gcaggacgca ccgccggcgc tgcagcaggc cctgtcgcag ggcccgcgcg ccgccctgac
     5341 ccgccgcccg gactttcagt tcgacgcccc gctgggccgg cgcaagctgc tggaacagtt
     5401 gcaggccgcc agcctggccg gctgggacgc cgaagagctg ccgcacgcgc acgccgcctg
     5461 cgccgccctg ctgagctacg ccgagcacac ccagggccgc gcgctgacgc acgtgcgcgc
     5521 cctggtggtc gagcgcgccg gcacgctgat cgagctgccc gccagcacgc gccgcaacct
     5581 ggagctggtg cagaccctgc gcggcgaaga cgcaccgacg ctgctgtcgc tgctcgacac
     5641 ctgcctgacc ggcatgggca gccggcggct gaaaagctgg ctgctggcgc ctgcgcgcga
     5701 gcgcgccgag gccagcgcgc gcctggccgc catcggcgcg ctgcgcgcag gcggcctgtg
     5761 gcagaagctg cgcgcccacc tgaagggcag cagcgacgtg gagcgcatca cggcgcgcct
     5821 ggcgctcggc caggtgcgcc cgcgcgagct ggtggcgttg cgccagacac tacaaaaaac
     5881 agagctgctg gcgcttgtcc agcaagcgcc agagccactt ttgatgcaga tcgccgcgga
     5941 tctggcaccg cccgccgagg cgctcgatct gctgcaggcg gcgctgctgc ccgagccggc
     6001 ggcgctgatc cgcgacggcg gcgtgatcgc ccccggtctg gaccaggagc tcgacgagct
     6061 gcgcgccatc agcgacaact gcgacgcctt tttgctggag ctggaagcgc gcgagcgcgc
     6121 gcgcaccggc atcgccaacc tgcgcgtgca gttcaacaag gtgcatggct tttacatcga
     6181 ggtcacgcag ggccaggcgg ccaaggtgcc cgacgactac cggcgccgcc agacgctgaa
     6241 gaacgccgag cgcttcatca cgcccgagct caaggccttc gaggacaagg cgctgtcggc
     6301 acaggagcgc gccctggcgc gcgaaaaatg gctgtacgag cacctgctaa accagctgca
     6361 gccgcacgtg ccgcagctca cccggctggg ccgcgccatt gccgcgctcg acgcgctggc
     6421 ggcgctggcc gagcgttcgc tgacgctgaa ctggtgcgcg cccgagttca cggcccaccc
     6481 ctgcatcgaa atccgcgccg gccgccaccc ggtggtgcag gcacgccttc aggagaccag
     6541 cggccaaccc ttcatcgcca acgacacgct gctgggcccc aagacgcgca tgcaggtcat
     6601 caccggcccc aacatgggcg gcaagagcac ctacatgcgc caggtggcgc tgatcgcgct
     6661 gctggcttcc gtcggctcct acgtgccggc gcaaagctgc cgcctggggc cgctggacgc
     6721 catccacacc cgcatcggcg ccgccgacga cctggccaac gcccagtcca ccttcatgct
     6781 ggagatgacc gaggcggcgc agatcctgca cggcgccacc gcccagtcgc tggtgctgat
     6841 ggacgagatc ggccgcggca ccagcacctt cgacggcctg gcgctggcca gcggcatcgc
     6901 cgcgcacctg cacgacaagg tgcaggcctt cacgctgttt gccacccact acttcgagct
     6961 gaccgagttt ccggccacgc accatgcggc cgtcaacgtg cacgtcgccg cggctgaaac
     7021 cgcgggctcg ggcggccacg acatcgtgtt cctgcacgag ctgcagccgg ggcccgccag
     7081 ccgcagctac ggcatccagg tggcgcggct ggccggcgtg ccggccgccg tggtccacca
     7141 cgcgcgccac gcgctggcgc agctcgaaca gggcgccagc gcggcgcagg tccaggtcga
     7201 cctgttcgcg ccgccgccgc ccgcggccga gccggcgccc tcgccgctcg acgcggcgct
     7261 ggccgcactc gaccccgacc agctcagccc gcgcgaggcg ctggacgcgc tgtaccggct
     7321 gaagaagctg gcgcgcgcgg gctgaaggcg gcgcctgcgc tgcgcgcatg agcgattgct
     7381 tcggaatctt gaccatggaa ccgttcaccc tgagcctgtc gcagggcttc gacaagctca
     7441 gccccaatgg cattcaagac atcccaaggc cgaaccaaga tgacatgacc tcacccggct
     7501 tcgatgactg ccgggggcgg ccggcagggc gagccggcac cgtggccagc gggcggccgg
     7561 gtcggctgca gcgcggtccc ggctcagccg ccccagaggc gataggccag ataggccagc
     7621 agcgccgcca tcagccagcc ccagaacgtg gtgccggtgc gcggcacttc gcccggcgac
     7681 aggccttcgg ccgccacctg cggcagctcg gcttccggcg ggcgcttgcc ggcaccggtg
     7741 gcgggccgcg ccgccggcgg cctggcgggc gccgccggtg gtgacgaggc ggccggccgc
     7801 tggctgagca ggaccttcag cagttgcggc aggttgcgct gtcggagcgc gtcgatcagt
     7861 tcgggcggca gcgagggggg tgtcggggtg ctcatggcgg ctttcgtcgg gggcgccagc
     7921 ggccggcacg gcccggggcg cgcgccgatt gtgaagcccg gccgccggct tggccagtcc
     7981 cccgggcggc gcgccgcggc gctgccccgg cacaatgccg ccccatgtcc accatcgtct
     8041 tttcccacgg caacagtttt ccggccagca cctaccgcgt gatgctcgac agcctgcggg
     8101 cgcgcggctt cgaggtgcac gcgatcgaga agtacggcca cgatccgaag tacccggtga
     8161 ccgacaactg gccgcacctg gtgcgccagc tggcgcagtt cgccacccag cgcagcaagg
     8221 acgaccacgg cggcgcgccc tggctggtcg ggcattcgct gggcggcctg ctgagcctga
     8281 tgtgcgcggc gcgccacccc gagctggcgc gcggcgtggt gatgatcgat tcacccgtgt
     8341 tgagcggctg gcgcgccggg gcggtgcgcg tggtcaagcg cacgcccctc atgcggcggc
     8401 tctcgcccag cagcgtggcg cgccggcgcc gcaaggaatg ggacgaccgc caggccgtgt
     8461 atgagcattt ccgtgccaag aaggtgtttg ccgcctggga cccgcgggtg ctgcgcgact
     8521 acgtggacca cggcacgctg gacgccgacg gcagctggcg cctggccttc gaccccgagg
     8581 tcgagtcgcg catctacgag accgtgcccc acaacctggg gccgctgctg cgccgccatc
     8641 cgctgcggtg cccggtggcc ttcgtcggcg gcatttcgtc ggtggagatg cgccaggtcg
     8701 gcgggctgga cgccaccgcc gaggtggcgc agggccgcgt gaccatgctg gaaggctcgc
     8761 acctgatccc gatggagcag ccgctggccg tggccgccgc catcgaggcg cacatccgcg
     8821 ccatgcctac ggcggcgacc gccgcacccg gaacgccagg ctgatgcgcg gccccagcgc
     8881 ctgcgcgcac ttgggcaccg catgcaggtg cgtgcgctgg ctggcgtggc tcatgcgcag
     8941 caggctgccc ggctccagcg tgatgcgcca gctggcaccg ccggccagcg cgcggatcag
     9001 catgtcgcgc ggcgccccca gcgacacgat ggcgatcggc cagcccggct ccagctcggc
     9061 ctgacggtcg ccgtgcggcg ccacgctgtc gcgcccgtcg cggtacaggt tcagccccac
     9121 atgggtgtag ggcgccggcg ccacggcctg cacctgcgcc agcgcggcgt cgatgcaggg
     9181 cgggcgcgcc gggtcgcgca acgccacgct ggcgaccagc cgcggcacct cgaccacgcg
     9241 ctcgtacatc ggccgctgca ggcggcgcca cggcgtctgc gccagcagcg ccgccagcca
     9301 cagcgcgccg ctggcctgcg gcacgcacgc cgggtggtag acgatgccgc cttcggcatc
     9361 gtgcaccagg gtgacggctt cgtcggcaaa cagcgaacac tgttgatgca tacagtcatg
     9421 ctagtgcagt gtttcgcgct gtcaagcact tatcctagaa acggcagttg accgcttgtc
     9481 agctgccgtt tctaggctca gggtgaacgg ttcagatttg aagattccgc agcataagat
     9541 cgcgggccgg ccacccggcg atcgtcagca cccatgaacc cggcttccac caccaccttc
     9601 tcgcaggccg cctggcaggg caacctggcg ctctaccacg acaccctggc cctgcccttc
     9661 aaccaggagc tggccgccgg caccctgagc gtcgagcgct ttcaccacta catcatccag
     9721 gacgcgcact acctggtggc ctacggccgc gcgctggcgg tggccgccgc caaggccgac
     9781 gacgccgacg ggctgatgca gttcaccgag gcggccaagg tcgcggtgct ggtcgagcgc
     9841 cagatgcacg gcggcttcat gcagcgtttc ggtgtgacga ccgagcagtt cgccgccacg
     9901 ccgctcacgc ccgcctgcca ccactacacg cagttcctgc tggccaccgc ctgggccgag
     9961 ccgtacccgg tggtgctggc cagcctgctg ccctgcttct ggatctacgc cgaagtgggc
    10021 cgggccatcc atgccagcgc gcgccagccc aacccctacc aggactggat cgacacctac
    10081 gccggcgagg aattccacac cgccgtgcgc ggcgtgctgg ccaccatcga ccgcgtggcc
    10141 gacggtgtgg atgcgcgcac gctgcaggcc atgcacgcgg cctacaccat gagtgcgcgg
    10201 ctcgaatgga tgttctggga ctcggcgcac cggctgggcc gctggccggt gtgacgccgc
    10261 cgtgcgcggt ggcggcgcgc gcgcatgacc aatgacctcg ctgcgcttgg acgaccaatg
    10321 gcccaacagt cgttcgtcat gccggcgcca accggtgtca tgggtcagct gccagaacgc
    10381 gaggcggcgt caaccggctt gcagcaccag cgacgcgcca aacgccaggc cttcggccgg
    10441 ccaatccgac gcggcgccga cctggcgctg gccgcgcgcc accaggcgcg cggcgcggat
    10501 cacgccggca tgcgtgaccc acgccgcctc gcgcccgctg gcgcgccagt cgtcccaggc
    10561 cagcgccacg cgcgccatca gctcggccac gcactcgccg ccgccggggc gggccagggc
    10621 aaagtcggcc agccaggcgt cgaactccgc tcgggcgatg tcgccccagg gccggccttc
    10681 ccaggtgccg aagtcgaact cgcgcagccg ggcgtcgaat ttcaaggtca aatcgggtcg
    10741 cagcgcttgc agggattgcg ccagcagctc acatctttgt agtggcgata cctgcaggca
    10801 cagcccgtgc ggcaggcgct cggccagcgc ccgcgcggcg gcacgggtgg cggcggcatc
    10861 ggccggcagg tcgctggcgc catagcacag gccgggcggc gccgccacct cggcatggcg
    10921 cagcagccac agcctcatgc cagcgccagc cccagataca gcgccagttc acacaactgc
    10981 tgggtggcgc ccagggtatc gccggtaaag ccttgcaggc gccgccgcag ccagcgcagc
    11041 acggccatca gcgtcagcgc ccacagcgcc atggcaactg ttgtttcggt agcgccttgc
    11101 gaccacccca tcagcgccag ggccggaatc gaccacaaaa cagaggtaac gacgctggtg
    11161 ccggacaccg catcggccag tggcttggac ttggcctgcg gcccttcggt gatgtagggc
    11221 agcacccgca tcaccgccag cggcgccagg cgcgacagca catgggcggc cagcagggcg
    11281 gccgccgcac cagccacacc gcgttgcagc agcaaagcca gcagcgccag cttgagaccc
    11341 agcgccagca ccagcgccag cgtgccgtag ctgccgatgc gcgagtcctt catgatctcc
    11401 agcgcgcgct ctcggctgac ctgcccgccc agcgcgtcgc aggtatcggc caggccgtct
    11461 tcgtgaaagg caccggtcag ccacgccgtg gcggcggtcg acagcccggc cgccaccagc
    11521 gcgcccagcg ccccggccgg cagccgcagc gccgccagcc acagcaccgc cgcgcccacc
    11581 gcgcccacca cccagcccac gcccggaaag tgcccggcgc tggcgttgag ctgggccggg
    11641 ctgtagccca cccacgccgc cagccggccg gtgaccggga tgcgggtgaa gaactgcagg
    11701 gcgagcaggt agtggcgcag ggcggccatg ggcaactcag cgcccggccg cctcgtcgtc
    11761 gatggcgcgg cgcacggtct gatagaaggc ttcgcgcgcg gcgtactgcg ggttgttcgc
    11821 cgagccggcc ttggcccagt cgccgttggt gacgatcacc agctcgcgcg ccgggtcgat
    11881 gaagatgccc tgaccaaaga tgccgcgggc ggcaaagctg ccgtcgtcat aggtccacca
    11941 ctggtagccg tagccgcggc ccggcgcgcc gatgtcggcc tgcttgtgcg tggcctgctg
    12001 cagatagccc tcgggcagga tggaagcgcc cccacgctcc acggcttcgc cggttgcgct
    12061 gccccccgag ggggccgccg ccggccttgg gagcggcccg gcggccggct ggccgtcgac
    12121 ttgcgcgcct tccagaatga actgcccaaa gcgcgcgtag tcgcgcggcg cggcctggat
    12181 gcagcagccg ctgatctcct
//
LOCUS       DAOOOG010000066        12035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_5885_length_12035_cov_23.451920, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000066 DAOOOG010000000
VERSION     DAOOOG010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12035
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5885_length_12035_cov_23.451920"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PK242_06620"
     CDS             complement(<1..340)
                     /locus_tag="PK242_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93728.1"
                     /translation="MPGPTERRLLTLALNVMLAVAILAAGVLAWRFAGGPPPDGPPGV
                     RVARLPPGSFVWASAPDDPRYLPDGLRAQDAGRLALLLLRERDGSLRAFYLPRQGGRA
                     SVPAAASPAVA"
     gene            complement(530..1210)
                     /gene="rpiA"
                     /locus_tag="PK242_06625"
     CDS             complement(530..1210)
                     /gene="rpiA"
                     /locus_tag="PK242_06625"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HOZ93729.1"
                     /translation="MTQDELKAMVGRAALEYVEAGQVVGVGTGSTVNHFIDALASMKE
                     RIPGAVSSSEASTARLQALGIPVFDCNAVGEIAVYIDGADEIDPRGHMIKGGGAALTR
                     EKIVAAQSRRFVCIADDSKRVEVLGRFPLPVEVIPMAAQRIVRQFAALGGQAELRHKD
                     GAPLVTDNGQHILDVRGLRIGDPLAFESTVNQWPGVVTVGVFAHQKAQICLLGTSQGV
                     RTLRFDGA"
     gene            1256..2353
                     /locus_tag="PK242_06630"
     CDS             1256..2353
                     /locus_tag="PK242_06630"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOZ93730.1"
                     /translation="MALLPYGLSRAVLFNMDPEAAHDLTIDLLARGAGTPLQWTWCHS
                     RIDDPVTLAGLALPNRVGLAAGLDKNARAIDGLAAMGFGFVEVGTVTPLAQPGNPRPR
                     MFRLPAAQALINRMGFNNDGLDAFVRNVRNSRFHSASRTPDGRQRMILGLNIGKNAAT
                     PIERATDDYLIALAGVYPFADYVTVNISSPNTKNLRDLQSDAALDALLSSLKSRQGEL
                     AAEHGRQVPLFLKIAPDLDGEQVQAIADALRRHGIDGVIATNTTLSREAVAGLPHADE
                     AGGLSGAPVREASNRVIGELRAALGAGYPIIGVGGIMSGADAVAKIEAGADAVQIYTG
                     LIYRGPGLVGEVARALRRSRAAGTTAQAAAR"
     gene            complement(2335..4164)
                     /locus_tag="PK242_06635"
     CDS             complement(2335..4164)
                     /locus_tag="PK242_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ93731.1"
                     /translation="MAVLTHAGTLPELTLPQMLRERARRDAQRVAIRQKEFGIWKPYT
                     WAQYYERANHFAQGLVALGMAPGGHLGVISENRIEWVLAQMGASLLGAVTVGVYPTSP
                     TSEVAYVVGHADIDVVVCEDQEQVDKVLEARAQLPRLRRIIMVETKGLRDYPPEQRAL
                     ISSFDDVERLGAQAGHQGLIEDALARQRLDDIGLMIYTSGSTGKPKGAMISWRNMRGV
                     VPGIVDRLALDADASHLSYLPLCHVAEQMLTTFVPIYLGSQVNFGESIRTVQEDLREV
                     APSMFLGVPRIWEKLHASISIKMQETGALRRALFERALRACAPLAQLPRDRWSLAERA
                     RFACAYWLVFRALQNFCGLRKVRVALTGAAPIAPEVVRFFRTLGVPLIEVYGLTESSG
                     MVTGHRLHDVVVGTVGPVTAGVEHRVADNGELQIRGEMVFAGYYKSPEATAQTIVDGW
                     LHTGDVVREEGGQIRIVDRLKDIMITAGGKNLTPSEIENAMKASPYVKECIVIADGRK
                     FVSALVQIEFDTVAKWAEARRIAFTHFRSLVETPEVRQLIEDEVAKGNARLAQVAQVR
                     RFHLLTKELDHDDGEVTATMKVRRASIYKAYAAEIEALYRAAA"
     gene            complement(4152..4973)
                     /locus_tag="PK242_06640"
     CDS             complement(4152..4973)
                     /locus_tag="PK242_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ93732.1"
                     /translation="MNPRTTDLILEINNIEVVYNKAVQALRGLSLAVPRGQIVALLGS
                     NGAGKSTTLKAVSNLLTLENGALESGSIRFQGQDTARRAPQELVRAGLSHVMEGRHVF
                     EDLTVEENLVAASYALTGRAGAARPDFDLVYHFFPRLHERRKGLAGYLSGGEQQMLAI
                     GRALIAQPQLMLLDEPSLGLSPKLVEEIFGIIVRINAERATSMLLVEQNATVALAVAH
                     SGYIMENGKIVIDGSADRLASDADVREFYLGMGGGGETRSFREIKHYKRRKRWLS"
     gene            complement(5059..6246)
                     /locus_tag="PK242_06645"
     CDS             complement(5059..6246)
                     /locus_tag="PK242_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ93733.1"
                     /translation="MAQQSFSNRRRSLMLGTAAAGAAALTQPLSVLAQSAEEIVIGGS
                     IPMTGVFAFAGVGINDGIQDYVKIINDAGGIKGRKVKYVPEDTGYKVDVSVAAFKKIT
                     SQNKVSLYYGDSTGFSKTINPELERNGNILMAGASFASELNDPKKYPHQFLVGPDYTE
                     MFGILLKHIAKEKPGAKVAFVYSDSEFGRDPIEKSEAAAKALGLSVPIKIMTAAGSVD
                     VSTEVIKLRRAAPDYTIFHGYILAPIPEFITQGKQQGMTSKWMGTFWTMDSSTVMKMG
                     PDGDGFMGVMPYRYYYDTEKAPMLEKIRAMRPQYQSTAYTQGMLAAMLFLEAAKRCLE
                     GNKPLTGVNLKAALNSIRDFDTGGLIGVPISIPGNSIPVGRVYKADMKAQKMVAASDW
                     IKL"
     gene            complement(6291..7328)
                     /locus_tag="PK242_06650"
     CDS             complement(6291..7328)
                     /locus_tag="PK242_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ93734.1"
                     /translation="MRIGTFKESYVADAALFDSRTQRAWLMVAAAALVLLPFVANDYW
                     LYMACLVAINIASSTGLNLLTGYTGLVSLGQAAFMGLGAYTVAILQIRWGTPLLLNLV
                     AGGAVAMLGGIVVGVPSLRVKGLYLAIVTIAATFIAHFLFANFEFTGGTSGLSMSPGR
                     LFGVELDSSFRLYWLIVPLTILMLLGSANLFRTRIGRAFIAIRDRDISAEVLGIPLLR
                     YKLLSFGLSSFYAGVAGGLWAYFFRVITPESFPLSMSIFFLAAIIVGGMGSTLGSILG
                     AAFMTMVPEVLKLVFDLLPASTDLAVLLSPVRLIIFGALIVGFLIFEPLGLAEMWRRT
                     RRFFHLWPFRN"
     gene            complement(7339..8217)
                     /locus_tag="PK242_06655"
     CDS             complement(7339..8217)
                     /locus_tag="PK242_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ93735.1"
                     /translation="MDWGYLFEVCLTGLAGGGLYALAALAFVLVYKATRVVNLAIGEL
                     LMAGGYLFFTFAVMWALPLWLAIPAAVLASGMLGGLIERLVIRPLLGEPPISAFMVTV
                     GLGSILVGLVEIIWSADQRRLPDFMPSKPIMVGDAFLAPKVFWGAVIAAVFIAAVLLV
                     FRYWRGGVALRATASDQAAAYVVGINVPRVFSLSWVVSAMLAAVSGIIVGSIGGISSS
                     MGVFGLSVLVVVIVGGLDSVLGALVAGLIVGLVEGLAAAYLGGEYKLLATFVVLVSIL
                     MVRPYGLFGTRDIERL"
     gene            complement(8217..9080)
                     /locus_tag="PK242_06660"
     CDS             complement(8217..9080)
                     /locus_tag="PK242_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ93736.1"
                     /translation="MATAEPLGSSDTKAPLLAVQDLTLAFGGVRALSGVGFDVQQGSI
                     TAVIGPNGAGKTSLFNTISGFYRPAAGHVRFKGQDITRVPAPLRARMGLGRSFQNIAL
                     FRGMTVLDNIKLGRHAHLKTNVFDALLYLGRARREEAELRRDIEERIIDFLEIDHIRH
                     APVSALSYGLQKRVEMARALAMQPEVLMLDEPVAGMNREETEDMARFILDVRAEWGVT
                     VLMVEHDMGMVMDLSDHVVVLNFGQAIAQGTPAVVQADPEVARAYLGSGDVHELRRKL
                     QAAAVAHQGAS"
     gene            9213..9938
                     /locus_tag="PK242_06665"
     CDS             9213..9938
                     /locus_tag="PK242_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ93737.1"
                     /translation="MAKTATSPPARRRTRALPVVESPWGPADRRQQQRQTKRNAVLST
                     AAEMFNERGFHATSLDDIAARLHVTKPTLYYYVKNKDEILLECVRKGLHLTLEGIEAS
                     RAAGGRAIDQLRACMLAYGHVVTQPFGMCVIRVGDQEVPVESRIELRRLKSLIDQAFR
                     RLVAAGVEEGSLRPCDPKLTAFTIAGGLSWIGRWYQPGGERSGDEIVQQCVATLMDGV
                     VHRAHTVPPSKPGRRQRPSAASA"
     gene            complement(9963..11213)
                     /locus_tag="PK242_06670"
     CDS             complement(9963..11213)
                     /locus_tag="PK242_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOZ93738.1"
                     /translation="MLSLLGAGSPALAQARTPAVEPIRLALIESLSGPFANTGEAVHR
                     NLVWAVERVNARGGVRTAGGPRPLELLRFDNKGQTEETLAMLRAAIDNGARVVLQGNS
                     SSAAAALIDTIDKHNARDPQRRVLFLNYSAVDPALTNAGCSYWHFRFDAHADMRMTAL
                     MQLLQEDVQLARIYLIGQDYSFGHAVLREARQQLAARRPDVAIVGDELHPVGRVKDFM
                     PYAVKIKASGAQAVLTGNWGNDLTLLVKAARDAGFDGRFYTFYGNALGVPAAIGDAGI
                     GKVVAVADWLPNLPTPESAAFYRAFRQRFPRPQDDYVHLRMQLMIEALAQAIERAGGD
                     DAAMLAQALEQADVTLAGQRGRMRADDHQFQQPLAVGLMERQGAPGVPFDVEGSGYGF
                     RVVRRIDARSAAMPHSCKMARPAH"
     gene            complement(11340..11933)
                     /locus_tag="PK242_06675"
     CDS             complement(11340..11933)
                     /locus_tag="PK242_06675"
                     /inference="COORDINATES: protein motif:HMM:NF021515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HOZ93739.1"
                     /translation="MSWVVMWRRWWVGVAPWGLTACVGWPAATAPLHDYQPERFDGAA
                     YVRHFKVDPTRSCEAARRALLSQGYVVTSTQAEQVSGRKYFQPSPEHHVQLEFRVVCA
                     SETDDAEAAVVFVSGLREQYIVRKNKESASVGVGGLGSLSLPVEGSMDSMVKVASETV
                     TDAALYRRFFELLGTYLDRVAVPERRTPQAATPERLS"
BASE COUNT         2102 a         4204 c         3888 g         1841 t
ORIGIN      
        1 cggccaccgc tggcgaggcg gccgccggca cgctggcgcg cccaccctgg cgtggcagat
       61 agaaggcgcg caggctgccg tcgcgctcgc gcagcagcag cagcgccaga cggccggcgt
      121 cctgggcgcg caggccgtcg ggcagatagc gcgggtcatc gggcgcgctg gcccagacga
      181 agctgccggg cggcaggcgc gccacgcgca cgccaggcgg gccatcgggc ggcggcccgc
      241 cagcaaaccg ccacgccagc accccggccg ccagaatcgc cacggccagc atgacgttca
      301 gtgccagcgt caacagtcgc cgctcggttg ggccgggcat ggtgtgtcga tcgacgacgg
      361 gccctcagcc gcagagtggc cgttggacgc gcccaccggt gccgtgtccg gggtggcggg
      421 caagccgcaa ggacgcggca tgctcacccg gccggtgatg gggatcgacg cgacgagacc
      481 cggcggtcat gcgtctgtcg cgaagatggc gagcgaccag ttgccgcact caggcaccat
      541 caaagcgcag cgtccgcacg ccctgggacg tgcccagcag gcagatctgc gccttttggt
      601 gtgcgaacac gcccacggtg accacgcccg gccattggtt gacggtgctt tcgaacgcca
      661 gcggatcgcc gatgcgcagg ccgcgcacgt ccaggatgtg ctggccgttg tcggtcacca
      721 gcggggcgcc gtctttgtgg cgcagttcgg cctggccgcc cagggcggca aactggcgca
      781 cgatgcgctg cgccgccatc ggaatcactt ccaccggcag tggaaaacgc cccagcacct
      841 ccacgcgctt gctgtcgtcg gcgatgcaga cgaagcggcg cgactgggcg gccacgatct
      901 tctcgcgcgt cagcgctgcg ccgccgccct tgatcatgtg gccacgcggg tcgatctcgt
      961 cagcgccgtc gatatagacg gcgatctcgc ccacggcgtt gcagtcgaac accggaatgc
     1021 ccagggcctg caggcgcgca gtcgacgctt ccgacgagga cacggcgccg gggatgcgtt
     1081 ccttcatgct cgccagcgcg tcgatgaagt ggttcaccgt cgaacctgtg cccacgccta
     1141 ccacctggcc cgcctcgacg tactcgagcg cggcgcgccc gaccatggct ttgagttcgt
     1201 cctgagtcat ttgcgacaat ttccctctgt tcccgtttca agcaagaatt atccaatggc
     1261 cctgctgccc tacggcctgt cgcgcgccgt cctgttcaac atggaccccg aggccgcgca
     1321 cgacctcacc atcgacctgc tggcgcgcgg tgccggcacc ccgctgcagt ggacctggtg
     1381 ccacagccgc atcgacgacc cggtgacgct ggccggcctc gccctgccca accgcgtggg
     1441 cctggccgcc gggctggaca agaacgcacg cgccatcgac ggtctggcgg cgatgggctt
     1501 cggctttgtc gaagtcggca ccgtcacccc gctggcacag cccggcaacc ccagaccgcg
     1561 catgttccgc ctgcccgccg cgcaggccct gatcaaccgc atgggcttca acaacgacgg
     1621 gctggacgcc tttgtgcgca acgtcagaaa ctcccgtttt catagcgctt cgcgcacgcc
     1681 agacgggcgc caacgcatga ttcttggcct gaacattggc aagaacgcgg ccaccccgat
     1741 cgagcgcgcc accgacgact acctgatcgc cctggccggc gtctacccgt ttgcagacta
     1801 cgtcacggtc aacatctcca gccccaacac caagaacctg cgcgatctgc agagcgacgc
     1861 cgcgctggac gcgctgctgt cgtccctcaa gtcgcgccag ggcgaactgg cggccgagca
     1921 tgggcggcag gtgccgctgt tcctgaagat cgcgcccgat ctggacggcg agcaggtgca
     1981 ggccatcgcc gacgcactgc gccgccacgg catcgacggc gtgattgcca ccaacaccac
     2041 gctgtcgcgc gaggccgtgg ctggcttgcc ccatgccgac gaggccggcg gcctgtcggg
     2101 cgcgccggtg cgcgaagcca gcaaccgcgt cataggcgag ctgcgcgcgg cgctgggcgc
     2161 cggctacccg atcatcggcg tgggcggcat catgagcggt gccgacgccg tggccaagat
     2221 cgaggccggc gccgacgcgg tgcagatcta caccgggctg atctaccggg ggcctggcct
     2281 ggtgggcgaa gtggcgcggg cgctgcgccg cagccgcgct gcgggcacca ccgctcaggc
     2341 ggcagcgcga tagagcgcct cgatctcggc cgcgtaggcc ttgtagatgc tggcgcggcg
     2401 caccttcatg gtggcggtga cttcgccgtc gtcgtggtcg agttcctttg tcaggaggtg
     2461 gaagcgccga acctgtgcca cctgggccag gcgggcattg cccttcgcca cctcgtcttc
     2521 gatcagctga cgcacttcgg gcgtctcgac cagcgaacgg aaatgcgtga aggcgatgcg
     2581 acgcgcctcg gcccatttgg caacggtgtc gaactcgatc tgcaccagcg cgctgacgaa
     2641 cttgcgacca tcggcgatga cgatgcactc cttgacgtag gggctggcct tcatcgcgtt
     2701 ttcgatttcc gacggcgtca ggttcttgcc acccgcagtg atcatgatgt ccttcaggcg
     2761 gtcgacgatc ctgatctgac cgccctcctc gcgcaccacg tcaccggtgt gcagccagcc
     2821 atcgacgatg gtctgcgccg tggcttcagg gctcttgtag tagccggcaa acaccatctc
     2881 accgcgaatc tgcagctcgc cgttgtcggc cacccggtgt tcgacccccg cggtgaccgg
     2941 cccgacggtg cccaccacca cgtcgtgcaa acgatggccg gtgaccatgc cggaggattc
     3001 ggtcagaccg tagacttcga tcagcggcac ccccagcgtg cgaaagaagc gcaccacttc
     3061 gggcgcgatc ggcgccgccc ccgtgagcgc cacccgcacc ttgcgcagac cacagaagtt
     3121 ctgcagtgca cgaaacacca gccagtacgc acaggcaaag cgcgcccgct cggccagcga
     3181 ccaccgatcc cggggcagtt gcgccagcgg cgcgcaggcc ctcagggcgc gctcgaacag
     3241 tgcgcggcgc agcgcgccgg tttcctgcat cttgatcgag atcgaggcgt gcagtttctc
     3301 ccagatgcgc ggcaccccca ggaacatgct gggcgccact tcgcgcaggt cttcctgcac
     3361 ggtgcggatc gactcgccga agttcacctg cgagcccagg tagatcggca cgaaggtggt
     3421 cagcatctgc tcagcgacgt ggcacagggg caggtaggac aggtgtgaag cgtccgcgtc
     3481 gagcgccagc cgatcgacga tcccgggcac cacgccccgc atgttgcgcc acgagatcat
     3541 ggcgcccttg ggcttgccgg tggaccccga ggtgtaaatc atcaggccga tgtcgtccag
     3601 ccgctggcgc gccagtgcgt cctcgatcag gccctggtgg ccggcctgcg cacccagccg
     3661 ctccacgtcg tcgaagctgg agatcagcgc gcgctgctcg ggggggtagt cgcgcaggcc
     3721 cttggtttcg accatgatga tcctgcgcag gcgcggcagc tgcgccctcg cctccagcac
     3781 cttgtccacc tgctcctggt cctcgcagac cacgacatcg atgtcggcgt gccccaccac
     3841 gtaggccacc tccgacgtcg ggctggtggg atagacgccg accgtgacgg cacccagcag
     3901 gctggcaccc atctgtgcca gcacccattc gatgcggttc tccgaaatca cgcccaggtg
     3961 cccgcctggt gccatgccca gcgccaccag accctgcgcg aaatggttcg cgcgctcgta
     4021 atactgtgcc caggtgtagg gcttccagat gccgaattcc ttttgccgga tcgccacgcg
     4081 ctgtgcgtcg cgccgggcgc gctcgcgcag catctgcggc agggtcagtt cgggcaaggt
     4141 gccagcgtgg gtcaggacag ccatctcttg cgccgcttgt agtgtttgat ctcgcgaaag
     4201 ctccgggttt cgccgccgcc gcccatgccc aggtagaact cgcgcacgtc ggcgtcgctg
     4261 gccaggcgat cggcgctgcc atcgatcacg atcttgccgt tttccatgat gtagccgctg
     4321 tgcgccacgg ccagcgccac ggtggcgttc tgctcgacca gcagcatgga ggtcgcccgc
     4381 tcggcgttga tgcgcacgat gatgccgaag atctcttcca ccagcttggg cgacaggccc
     4441 agcgaaggct cgtccagcag catcagctgg ggctgggcga tcagcgcgcg cccaatcgcc
     4501 agcatctgct gctcgccgcc ggacagatag cctgccagac ccttgcgccg ctcgtgcagg
     4561 cgcggaaaga agtggtagac gagatcgaaa tccggccggg ccgcaccggc gcgcccggtc
     4621 aacgcgtagc tggcagcgac gaggttttcc tccaccgtca gatcctcgaa cacgtggcgc
     4681 ccttccatca cgtgcgacag gccggcacgc accagctcct gcggcgcacg gcgcgccgtg
     4741 tcctgaccct ggaaacggat gctgcccgac tccagcgccc cgttctccag cgtcagcagg
     4801 ttggacacgg ccttcagcgt ggtgctcttg ccggcgccgt tgctgcccag cagcgccacg
     4861 atttgcccgc gcggcacggc cagcgacagg ccgcgcaggg cctgcaccgc cttgttgtag
     4921 accacctcga tgttgttgat ttcgaggatc aggtcggtcg tgcgcgggtt catgggggtc
     4981 acccctggcc tcgacgtgtt ccaaccctgg ccccgccgac cgggcgggaa cacgccgggt
     5041 cggcgttccg ggtcaggctc acagcttgat ccagtcggat gcggccacca tcttctgtgc
     5101 cttcatgtcg gccttgtaga cgcgaccgac cggaatcgag ttgcccggga tgctgatcgg
     5161 cacgccgatc aggccgccgg tatcgaaatc gcgaatcgag ttcagcgccg ctttcaggtt
     5221 gacgcccgtc aggggcttgt tgccttccag gcagcgcttg gccgcctcca ggaacagcat
     5281 ggcggccagc atgccctgcg tgtaggcggt gctctggtac tgcggccgca tggcacggat
     5341 cttctccagc atcggcgcct tctcggtgtc gtagtagtag cggtagggca tgacgcccat
     5401 gaagccgtct ccgtccgggc ccatcttcat gacggtggag ctgtccatgg tccagaaggt
     5461 gcccatccat ttgctggtca tcccctgctg cttgccctgg gtgatgaact cggggatggg
     5521 tgccaggatg tagccgtgga aaatcgtgta gtcgggcgcg gcgcggcgca gcttgatgac
     5581 ttcggtggac acgtccacgc tgccggcggc ggtcatgatc ttgatcggca ccgacagacc
     5641 cagggccttg gccgcggcct cgctcttttc gatcgggtcg cggccgaact ccgagtccga
     5701 gtacacgaag gccaccttgg cgcccggctt ttccttggcg atgtgcttga gcaggatgcc
     5761 gaacatctcg gtgtagtccg gcccgaccag gaactggtgc gggtatttct tggggtcgtt
     5821 gagctcgctg gcgaacgacg cgccggccat cagaatgttg ccgttgcgct ccagctccgg
     5881 gttgatggtc ttggaaaagc cggtcgaatc gccgtagtac aggctgacct tgttctggct
     5941 ggtgatcttc ttgaaggccg ccaccgacac gtccaccttg taaccggtgt cctcgggcac
     6001 gtacttgacc ttgcggccct tgatgccgcc ggcgtcgttg atgatcttca cgtagtcctg
     6061 gatgccgtcg ttgatgccca cgccggcaaa ggcgaacacc ccggtcatcg gaatcgagcc
     6121 gccgatgacg atctcctcgg cgctctgggc cagcaccgac agcggctgcg tcagtgcggc
     6181 ggcgcccgcc gcggccgtgc cgagcatcag gctgcggcga cggttggaaa aggattgctg
     6241 agccatgaat gtctccttga agcaggacgt tgcgaaaaaa tgacccgcgc tcagttgcgg
     6301 aagggccaca ggtgaaagaa ccgtcgtgtg cggcgccaca tctcggccag acccagcggc
     6361 tcgaagatca ggaagccgac gatcagggcg ccaaagatga tcaggcgcac cggcgacagc
     6421 agcaccgcca ggtcggtgct ggcgggcagc aggtcgaaca ccagcttgag cacttccggc
     6481 accatcgtca tgaaggccgc gcccagaatg ctgcccagcg tcgaacccat gccaccgacg
     6541 atgatggcgg ccaggaagaa aatcgacatc gacagcggaa agctctcggg cgtgatgacg
     6601 cgaaagaaat acgcccacaa cccgcccgcc acaccggcgt agaacgaaga cagcccgaac
     6661 gacagcagct tgtagcgcaa cagcggaatg cccagcacct cggccgagat gtcgcggtcg
     6721 cggatggcga tgaaggcgcg gccgatgcgc gtgcgaaaca ggttggccga acccagcagc
     6781 atgaggatcg tcagcggcac gatcagccag tacaggcgaa acgagctgtc cagttcgaca
     6841 ccgaacaacc gcccgggcga catggacaac ccgctggtgc cacccgtgaa ctcgaagttg
     6901 gcgaacagaa aatgggcgat gaaggtggcg gcaatggtga cgatcgccag gtacaagccc
     6961 ttgacgcgca gcgacggcac gccgaccacg atgccgccca gcatggccac tgcgccgccc
     7021 gccaccaggt tgagcagcag cggcgtaccc cagcggatct gcagaatggc caccgtatag
     7081 gcccccaggc ccatgaacgc cgcctgcccc aggctgacca gaccggtata gcccgtcagc
     7141 aggttcagcc cggtgctgct ggcaatgttg atggcgacca ggcaggccat gtacagccag
     7201 tagtcgttgg ccacgaacgg aagcagcacc agcgccgcgg cggccaccat cagccacgcg
     7261 cgctgcgtgc gcgaatcgaa cagcgccgcg tcggcgacgt agctttcctt gaaggttccg
     7321 atgcgcatga cgacgggcct acagcctttc gatgtcccgc gtgccgaaca ggccgtaggg
     7381 acgcaccatg agaatgctca ccagcaccac gaaggtcgcc agcagtttgt actcaccgcc
     7441 caggtaggcc gccgccagac cctcgaccag accgacgatc aaccccgcca ccagggcgcc
     7501 gagcacgctg tccaacccgc cgacgatcac caccaccagc accgacagac caaacacgcc
     7561 catggacgac gaaatgccac cgatcgaacc gacgatgatc ccggacaccg ccgccagcat
     7621 ggccgacacc acccaggaca gcgaaaacac ccgcggcacg ttgatgccca ccacgtacgc
     7681 cgcggcctgg tcgcttgccg tggcgcgcag ggccacgcca ccgcgccagt agcgaaacac
     7741 cagcagcacc gcggcaatga acaccgcggc gatcaccgcg ccccagaaca ccttgggcgc
     7801 gaggaacgcg tcgcccacca tgatgggctt gctgggcatg aagtccggca gccggcgctg
     7861 atcggcgctc cagatgattt ccaccaggcc caccaggatc gagcccagac cgacggtgac
     7921 catgaacgcc gaaatcggag gctcgcccag caggggccgg atcaccagcc gctcgatcag
     7981 gccacccagc atgccactgg ccagcaccgc cgccggaatc gccagccaca gcggcaacgc
     8041 ccacatcact gcgaaggtga agaacaggta gccgcccgcc atcaacaact cgccgatggc
     8101 caggttcacg acccgcgtcg ccttgtagac caggacgaag gccagcgccg cgagcgcata
     8161 caggccgcca ccggccagac cggtcagaca gacctcgaac aggtagcccc agtccatcag
     8221 ctcgccccct ggtgtgcgac tgccgccgcc tgcagcttgc gacgcagttc atggacgtcg
     8281 cccgagccca ggtaggcgcg cgccacctcg gggtcggcct gcaccaccgc cggcgtgccc
     8341 tgggcgatcg cctggccgaa gttgagcacc accacatggt ccgacaggtc catcaccatg
     8401 cccatgtcgt gctccaccat gagcaccgtc acgccccatt cggcgcgcac gtcgaggatg
     8461 aagcgcgcca tgtcctcggt ctcctcgcgg ttcatgccgg ccacgggctc gtcgagcatc
     8521 agcacctcgg gctgcatggc cagggcgcgc gccatctcca cgcgcttttg caggccgtag
     8581 gacagggccg agacgggggc gtggcggatg tggtcgatct cgaggaagtc gatgatgcgc
     8641 tcctcgatgt cgcggcgaag ctcggcctcc tcgcggcgcg cacggcccag atagagcagc
     8701 gcgtcgaaca cattcgtctt gaggtgcgcg tggcgcccga gcttgatgtt gtcgagcacc
     8761 gtcatgccgc gaaacagtgc gatgttctga aagctgcgcc ccagtcccat gcgggcgcgc
     8821 agcggcgcag gaacgcgcgt gatgtcctgg cccttgaacc gaacatgacc cgcggctggc
     8881 cggtagaagc ccgaaatggt gttgaacagc gacgtcttgc cggcaccgtt cggaccgatc
     8941 acggccgtga tgctgccctg ctggacgtca aaccccacac ccgacagcgc ccgcacgcca
     9001 ccgaaggcca gcgtcaggtc ttgcaccgca agcagaggcg ccttcgtatc tgagctgcca
     9061 agtggctccg ccgtcgccat gatgctgtgt tggggttaat tcgtatccca cgatctctac
     9121 ttgaccgtag actagttact cagaagtaaa tataccggtg gataccggcc aaccgggatc
     9181 ggtgttttcc cgatgaatct cagcacgcag acatggcgaa gactgcaacc tcccctcccg
     9241 cgcgccgacg cacgcgcgcc ctgcctgtcg tcgaatcgcc ctggggcccg gccgaccggc
     9301 gccagcagca gcgccagacc aaacgcaacg cggtgctgtc caccgcggcc gaaatgttca
     9361 acgaacgtgg ctttcatgcc acttcgctcg acgacatcgc agcccgcctg catgtgacca
     9421 aaccgacgct ctactactat gtcaagaaca aggacgagat cctgctcgaa tgcgtgcgca
     9481 agggcctgca cctgacgctg gagggcatcg aggcatcgcg cgccgccggc ggacgggcga
     9541 tcgaccaact gcgcgcctgc atgctggcct acggtcacgt cgtcacgcag cccttcggca
     9601 tgtgcgtgat ccgcgtcggt gaccaggagg tgccggtcga aagccggatc gagctgcgcc
     9661 gcctgaagtc cctcatcgat caggcgttcc ggcgcctggt cgccgccggg gtcgaagaag
     9721 gtagcctgcg cccctgcgac cccaagctga ccgcattcac catcgccggc ggcttgagct
     9781 ggatcggccg ctggtaccaa ccgggcggcg aacgttcggg cgatgagatc gtccagcagt
     9841 gcgtggcgac gctgatggac ggtgtcgtcc atcgcgcgca caccgtgcca ccgagcaaac
     9901 ccggccgccg acagcggccc tccgccgctt ccgcctgacg cgaaccagac ttcaggaaag
     9961 actcagtgcg ccggccgcgc catcttgcag ctgtgcggca tggcggcgct gcgcgcgtcg
    10021 atgcggcgca ccacacgaaa gccgtagccc gagccttcga cgtcgaacgg cacgccgggc
    10081 gcaccctggc gctccatcag gcccacggcc agcggctgct ggaactggtg atcgtcggcg
    10141 cgcatgcgcc cgcgctgacc ggccagcgtg acgtcggcct gctccagcgc ctgcgccagc
    10201 atggcggcat cgtcgccgcc ggcgcgctcg atggcctgcg ccagcgcctc gatcatgagt
    10261 tgcatgcgca ggtgcacgta gtcgtcctgc gggcggggaa agcgctggcg gaacgcgcgg
    10321 tagaacgcgg cgctctcggg cgtgggcaag ttgggcagcc agtcggccac cgccaccacc
    10381 ttgccgatgc cggcatcgcc gatggccgcc ggcacgccca gcgcattgcc atagaaggtg
    10441 tagaagcgcc cgtcaaaacc ggcgtcgcgc gccgccttga ccagcagcgt caggtcgttg
    10501 ccccagttgc cggtcagcac cgcctgcgcg ccggaggcct tgatcttcac ggcataaggc
    10561 atgaagtcct tcacccggcc gacgggatgc agctcgtcac cgacgatggc cacgtcgggc
    10621 cgccgcgccg ccagttgctg gcgcgcctcg cgcagcacgg cgtggccgaa gctgtagtcc
    10681 tggccgatca ggtagatgcg cgccagctgc acatcttcct gcagcaactg catcagcgcc
    10741 gtcatgcgca tgtcggcgtg ggcgtcgaag cgaaagtgcc agtagctgca gccggcattg
    10801 gtgagcgccg gatcgaccgc cgaatagttc agaaacagca cccgccgctg gggatcgcgc
    10861 gcgttgtgct tgtcgatggt gtcgatcagc gccgccgccg cgctggaaga gttgccctgc
    10921 agcaccacgc gggcgccgtt gtcgatggcg gcgcgcagca tggccagcgt ctcctcggtc
    10981 tgccccttgt tgtcaaagcg cagcagctcc agcggcctcg gcccgcccgc ggtgcgcacg
    11041 ccgccgcggg cgttgacgcg ctcgaccgcc cagaccaggt tgcgatgcac cgcttctccg
    11101 gtgttggcaa acgggccgct gagcgactcg atcaaggcca gtcggatcgg ttcgacagcg
    11161 ggtgtacggg cctgcgccag ggcgggcgag ccggcgccga gcagggacag catggcgccc
    11221 agggcggcgc gacggtgcaa gagatgtgac atgaagagaa ggagtcaaga acaacagaag
    11281 aacacacacg gccgtcgttg gccggagcag gccggacgac gccggcagga aagccgcaat
    11341 caggacaggc gttcgggcgt cgccgcctgc ggcgtgcgcc gctcgggcac ggccacgcgg
    11401 tccaggtagg tgcccagcag ttcgaagaag cgccggtaca gggcagcgtc ggtgacggtt
    11461 tcgctggcca ccttgaccat cgaatccatg ctgccttcga ccggcagcga cagcgagccc
    11521 aggccgccca cgccgaccga ggccgattcc ttgttcttgc gcacgatgta ctgctcgcgc
    11581 agcccgctga cgaacaccac ggccgcctcg gcgtcgtcgg tctcgctggc acacaccacg
    11641 cgaaattcga gctgcacatg gtgttcggga ctgggctgga agtacttgcg gcccgacacc
    11701 tgctcggcct gcgtgctggt caccacgtag ccctggctca gcagggcgcg ccgcgccgcc
    11761 tcgcacgaac gtgtggggtc gaccttgaag tggcgcacat aggcggcgcc gtcaaagcgc
    11821 tcgggctggt agtcgtgcaa gggcgccgtg gcggccggcc agccgacgca tgccgtcagc
    11881 ccccacgggg ccacccccac ccaccatcga cgccacatca ccacccaaga catccgaaac
    11941 gaccgcctga cgaaaaaccg tgaatgctaa cgcccggcag ggcagcgacg accccgcgcg
    12001 ggaaaagaaa agtcgccccg ctggcgcatc aggcc
//
LOCUS       DAOOOG010000067        11974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_5925_length_11974_cov_25.033392, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000067 DAOOOG010000000
VERSION     DAOOOG010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11974
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5925_length_11974_cov_25.033392"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(344..1681)
                     /gene="hslU"
                     /locus_tag="PK242_06680"
     CDS             complement(344..1681)
                     /gene="hslU"
                     /locus_tag="PK242_06680"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOZ93740.1"
                     /translation="MSSMTPQEIVSELDRHIVGQHDAKRAVAIALRNRWRRQQVDEKL
                     RQEITPKNILMIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGKDVDAIIRD
                     LAEMAVKQQREQAMKAHRTRAEDLAEDRILDVLLPPARQADGSNAPAGDSAARQAFRK
                     KLREGQLDDKEIELELAQPAPTMEILGPPGMEEMAEQLKGMLGNLGGGRRKLRRVRIG
                     EAMKLLVDEEAGKLVNEDDIRTEAVRNLEQNGIVFIDEIDKVASRGLEGGGSSAEVSR
                     QGVQRDLLPLVEGTTVSTKYGMVRTDHILFIASGAFHLSKPSDLIPELQGRLPIRVEL
                     ASLSVQDFEAILTQTDASLVRQYQALLATEGVTLDFRPEGISRLAEIACAVNEATENI
                     GARRLSTVMERLLEEVSFDASRRQGQTLTIDAAFVDARLAVLSQDEDLSRYIL"
     gene            complement(1769..2314)
                     /gene="hslV"
                     /locus_tag="PK242_06685"
     CDS             complement(1769..2314)
                     /gene="hslV"
                     /locus_tag="PK242_06685"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOZ93741.1"
                     /translation="MEAFHGTTIVSVRRQTPQGWQVAIGGDGQVTLGHIVVKGTARKV
                     RKLHHGRVLAGFAGATADAFTLFERFEAKLEKHQGHLTRAAIELTKDWRTDRVLRRLE
                     AMLAVADREASLIITGNGDVLEPEHGLVAIGSGGAYAQAAARALLAHTDLPAEQIVRK
                     SLEIAGELCIYTNMQHTIEVL"
     gene            complement(2379..4115)
                     /locus_tag="PK242_06690"
     CDS             complement(2379..4115)
                     /locus_tag="PK242_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOZ93742.1"
                     /translation="MNKPTEPGGLLSKVVRFVKNPTTHWADLDQPDSEDGDSQSRLAL
                     KEMIERKRRNDFVRSREFDMLRKLRRRETLKGSDLAAGPPSFYPSSHPANTGERELTL
                     KKIDEIEAQMSTAWSRHRVGKNQAAGPAADAISEPSVHAYDKTDPMPLPPPPEGGAPL
                     LDAAADGSASALAKESLSAQPLHPSAGGAAAARAIPWLGEVQDFNVEVMAAGRQDPEI
                     EEAAILFANGDGAGAEALLRALVAEGGARREDVDTWLTLFDLYRCIGDQARFDDAAPE
                     FAAQFGRSAPQWTGMLDRPSSGVSPLTAAPAPGATAAGPFHWISPSALGTQSMATLRM
                     SLERHAPPWRLDWRHLQSIEAAALPSLVEQLQRWGRTPVRLKLLGIERLMDVLTERSP
                     TGDRTVDPQWWEARLALLRVLGQMDEFDLVALNYCVTYEVSPPAWEDPVNGWTRMNEE
                     GETLMSSRFGSDTLGVRMPEPGGDEAPSHDTEEPPEGVVRTVLDGAYSGSMSALLQPL
                     AQRGEVRHFEFNCRQLVRVDFGAAGDLLNWATEQQGSGRSISFKQVNRLVAAFFGVIG
                     ITDVSRVLLRTD"
     gene            complement(4320..5015)
                     /gene="dksA"
                     /locus_tag="PK242_06695"
     CDS             complement(4320..5015)
                     /gene="dksA"
                     /locus_tag="PK242_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HOZ93743.1"
                     /translation="MKTAAAKSPATPKAKPAKAPSSAGKPASKARVSAPEPSKKSPVR
                     SAAAKASPAVPTKPPQPVSRNLPMPSVATLPMPAPVKKDPKLANNWKTKSVEQLSDPE
                     IIAMPDNEYMNDKQLAYFRLKLVQLKEDMLANADQTAENLRDDTVVVPDPADRATIEE
                     EHALELRTRDRERKLLKKIEQSIARIDAGDYGYCDETGEPIGVPRLLARPTATLSLEA
                     QQRRELKQKMFGD"
     gene            complement(5440..6501)
                     /locus_tag="PK242_06700"
     CDS             complement(5440..6501)
                     /locus_tag="PK242_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOZ93744.1"
                     /translation="MSLIPATILTGFLGSGKTTLLKRVLTEAHGQKIAVIENEFGEEN
                     IDNDILVSDSQEQIIQMSNGCICCSIREDLRATLSDLARRKRSGELDFERVVIETTGL
                     ADPGPVAQTFFMDDEIAEGYLLDAIVTLVDAKHAAQQLNDRIEAQRQVGFADQIFISK
                     ADLVDAGELAALQHRLKHMNPRAAQKVVHFGAVPIAEVFDLRGFNLNARLDIDPEFLA
                     ADDHDHDHHDHHHHHDGHCDHPSHQHAHGHHHHADDDVKSFVFRAERAFDPARLEDFL
                     GAIVNVYGPRMLRYKGVLWMKGTERKVIFQGVHQLMGSDLGPAWKGDEPRQSKMVFIG
                     IDLPRDIFTQGLEQCLVSA"
     gene            6803..7618
                     /locus_tag="PK242_06705"
     CDS             6803..7618
                     /locus_tag="PK242_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOZ93745.1"
                     /translation="MSKSFNLVALALSLGITGAQARELVLGISEGTSGGTDHARVVQK
                     YGGLAEAIGKAIKAEVKVVFVREFAQLEDGMKSARLDLVMARPSDYPARGLRDYGYHY
                     VATVKPEGQCLIVVPKDSPIKQLADLKGKKIVMPEKVAYMTKFCKAELRHQGVDLDKE
                     SVTYVREQEAVVFYINNGFGQVGGLASYSGAARKWLKEGGTVLHRSVPQPYSPLIASK
                     ALSAADIAAVQKAVLALPDSPEGQVVLKSIGMPGGFDTATEDKLRGLLKFLGD"
     gene            complement(7643..8617)
                     /locus_tag="PK242_06710"
     CDS             complement(7643..8617)
                     /locus_tag="PK242_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOZ93746.1"
                     /translation="MSSVVEPLPSAAAADAALVERYLTHARVLKRLAPRTLALYRADL
                     DKLARLAAQAGVALPQVQHHHVRSWVARMHAGGRSGRGIALILSGWRGFYVWLGREGG
                     IGANPVLGIRAPRQPRPLPKALGVDEAVQLAEHIDEGDDPWLDARDAAMVELLYGSGL
                     RVGELVGLDVAASDAAARAGRGWIDLDQAEVQVQGKGSKRRAVPLGRSAAEALRRWLA
                     VRGQVMAAGEAAAALFIGRRGTRLSAPSVWQRLRRRSQQAGLATPVHPHMLRHSFASH
                     VLQSSGDLRAVQELLGHASIGTTQVYTRLDFQHLAQAYDAAHPRARRR"
     gene            8983..9456
                     /locus_tag="PK242_06715"
     CDS             8983..9456
                     /locus_tag="PK242_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOZ93747.1"
                     /translation="MAAHPAHALALGFGSGLSPVAPGTAGTLWAWLAWWALGLWLSPA
                     QQGWLLVGALPLAWWACTVTAQHLQTPDPGAIVIDEVVAFWLVLWLLLPAGFWGQATA
                     FALFRFFDAVKPGPVGWADRLLHGRRGWAGGFGVLLDDLVAAFCTLLVLALWRPG"
     gene            9463..10008
                     /locus_tag="PK242_06720"
     CDS             9463..10008
                     /locus_tag="PK242_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOZ93748.1"
                     /translation="MLPKQELSALTGSAPEAKIVHDIAALLTARGWLLTTAESCTGGL
                     VAAACTELAGSSLWFERGIVSYSNAAKSELLGVPPALIARHGAVSEPVVRAMAEGALA
                     HARAQVSVALTGIAGPTGGSADKPVGTVWFGWCVDGRTDTQRCRFDGDRQAVRVQAAL
                     HALRGLAARLAAPGGAATPLT"
     gene            complement(10001..11584)
                     /locus_tag="PK242_06725"
     CDS             complement(10001..11584)
                     /locus_tag="PK242_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ93749.1"
                     /translation="MKLRTLAVAALAVCCTLGAHARTFKWTSASDIPTLDIHSQNNAL
                     GNGVHAAVYESLVYYNSKSFKIEPMLATAWKQVSPTQMQFTLRTGVKFSDGSSMTAED
                     VKFSIERAMAKTSNYSVYTQGIDKVVVAGPNTLDIFTKAPNPVLLNQLTELRVMSKAW
                     AEKNKSVEPKDIKTKDESFAHRNAMGTGPFMVKEWQPDQKLVLVDNPQWWGHGAKAKP
                     GNVTEVIYTPIKSEATRMAALLSGEVDFVLDPTPQDLERVQRNAALKVVQGPENRTIF
                     FGMDQHRDELPGSSVKGKNPLKDQRVRQALYQAIDINAIHKVVMRGLSQNTGTLIAPQ
                     VSGYTKRADQRYPYDAAAAQKLLAEAGYKDGFEVDFACPNNRYIADERICQAVAAMWS
                     KVGVKAKLRTMPLVSYFPMIQRHEASIYMLGWGVPTFDALYSLQSLVRSVGAGGDGNY
                     NVGRYSNPQMDALVERVKVEVDQKRRAELIEQALILEHKDVSHLPLHNQVIPWAMKKN
                     IEVVHRADNRLDWRLIAVK"
     gene            11794..>11974
                     /locus_tag="PK242_06730"
     CDS             11794..>11974
                     /locus_tag="PK242_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ93750.1"
                     /translation="MSSDIILETRGLVKEFKGFVAVNDVNLQVRKGTIHALIGPNGAG
                     KTTCFNLLTKFLEPTR"
BASE COUNT         1834 a         4045 c         4017 g         2078 t
ORIGIN      
        1 ctcgctcaac cggaaaagcc ttgaatgccg gaagtcgcgc cacgggcgcc gggcgtgatc
       61 gcccctgcag gacgcgggcc ggcatgcgcc attaccccct cctgtcaccc ggggaacggc
      121 agttggacgc gtccgcccgt gccgggatcg gcgtgccagg caagacgcga cgacgctgcg
      181 ggctcctgcc cgcaaggaga agcaacgccg catggcgcgg cgagcgcggt gcgggtgggt
      241 gcgcagcgcg ttcactcagg aggtgcaggc catcgcagcc gcaagacccc gcatcgatgc
      301 ggtctgcccg aggttggcaa gcggtcaact gccgtttcta ggatcagagg atgtagcgcg
      361 acagatcttc gtcctggctc agcaccgcca ggcgcgcatc gacgaaggcg gcatcgatgg
      421 tgagcgtctg cccctggcgc cggctggcgt cgaagctgac ttcttcgagc agccgctcca
      481 tgacggtgga caggcggcgc gcaccgatgt tttcggtggc ttcgttgacg gcgcaggcga
      541 tctcggccag gcgcgagatg ccctcggggc ggaagtcgag cgtcacgccc tcggtggcca
      601 gcagggcctg gtactggcgc accaggctgg cgtcggtttg cgtcaggatg gcctcgaagt
      661 cctgcaccga cagcgaggcc agctccacgc ggatcggcag ccgcccctgc agctcgggga
      721 tcaggtcgct cggcttggac aggtgaaagg cgccggaggc gatgaacagg atgtggtcgg
      781 tgcgcaccat gccgtacttg gtgctcacgg tggtgccttc gaccagcggc agcaggtcgc
      841 gctgcacgcc ctggcgcgag acttcggcgc tgctgccgcc gccctccagg ccgcgcgagg
      901 ccaccttgtc gatctcgtcg atgaagacga tgccgttctg ctccagattg cgcaccgcct
      961 cggtgcggat gtcgtcctcg ttgaccagct tgccggcttc ttcgtccacc agcagcttca
     1021 tggcttcgcc gatgcgcacg cggcgcagct tgcgccggcc accgcccagg ttgcccagca
     1081 tgcccttgag ctgctcggcc atttcttcca tgccgggcgg ccccaggatc tccatggtgg
     1141 gcgcgggctg ggccagttcc agctcgattt ccttgtcgtc cagctgacct tcgcgcagct
     1201 tcttgcgaaa ggcctgacgc gcggcgctgt cgccggccgg cgcgttgctg ccgtcggcct
     1261 gacgcgcggg cggcagcagc acgtcgagga tgcggtcttc ggccagatcc tcggcgcgcg
     1321 tgcggtgcgc cttcatggcc tgctcgcgct gctgcttgac ggccatctcg gccaggtcgc
     1381 ggatgatcgc atcgacgtcc ttgccgacgt agccgacctc ggtgaacttg gtggcttcga
     1441 ccttgatgaa gggggcgttg gcaagccggg ccaggcgtcg cgcgatctcg gtcttgccga
     1501 cgccggtggg accgatcatc aggatgttct tgggcgtgat ctcctggcgc agcttctcgt
     1561 cgacctgctg gcgccgccag cggttgcgca aggcgatggc cacggcccgc ttggcgtcgt
     1621 gctggccgac gatgtggcgg tcgagttcgg agacgatctc ctggggggtc atggaggaca
     1681 tggcgggagc gtgacagatg acaaacgact tcgtcgcggc gcgacgtcaa tggcgaacga
     1741 cacgaggtca ttcgccattc gtcatggttc agagaacctc gatggtgtgc tgcatgttgg
     1801 tgtagatgca caactcgcca gcgatttcga gcgacttccg gacgatctgc tcggccggca
     1861 ggtcggtgtg cgccagcagc gcccgggccg ccgcctgggc ataggcgccg cccgaaccga
     1921 tggccaccag gccgtgctcg ggctccagca cgtcgccgtt gccggtgatg atcagcgagg
     1981 cctcgcggtc agccaccgcc agcatcgcct ccagccggcg cagcacgcgg tcggtacgcc
     2041 agtccttggt gagttcgatg gcggcgcgcg tcagatggcc ctgatgtttt tcgagcttgg
     2101 cttcgaagcg ctcgaacagc gtaaaggcat cggcggtggc gccggcaaag ccggccagca
     2161 cccgaccgtg gtgcagcttg cgcaccttgc gcgccgtgcc cttgacgacg atgtggccca
     2221 gggtgacctg accgtcaccg ccaatggcca cctgccagcc ctgcggggtc tgacgccgca
     2281 cgctgacgat ggtggtgccg tgaaatgctt ccatatcttt tccagatgat ggtcagctgt
     2341 tgcgattcaa gtgcgggccc ttcccgcgcc tgtacccttc aatccgtgcg cagcagcaca
     2401 cgcgaaacgt cggtgatgcc aatgacgccg aagaacgccg cgaccagtcg gttcacctgc
     2461 ttgaagctga tgctgcgacc actcccctgc tgctcggtgg cccagttcag caggtcacct
     2521 gcggcgccga aatccacccg gaccaactgc ctgcagttga actcgaaatg ccggacctca
     2581 ccccgctgcg ccaagggctg cagcaaggca ctcatggagc cactgtaggc cccgtccagc
     2641 actgtcctga cgaccccctc aggtggctct tcagtgtcgt gcgacggcgc ctcatcccca
     2701 cctggctcgg gcattctcac ccctagggta tcgctgccga accgactcga catcagggtc
     2761 tcgccctcct cgttcatgcg ggtccagccg ttgaccggat cttcccacgc cggcggagac
     2821 acctcgtaag tgacacagta gttgagtgcc accagatcga actcgtccat ctgcccgagc
     2881 acgcgcagca gcgccaggcg ggcctcccac cactgcggat cgaccgtgcg atcgccggtg
     2941 ggtgaacgct cggtcaacac gtccatcaga cgctcgatgc ccagcagctt cagccgcacc
     3001 ggcgtccttc cccagcgctg cagctgctcg accaacgacg gcagtgcggc tgcctcgatg
     3061 gactggaggt gacgccagtc gagacgccag ggcggcgcgt ggcgctccag cgacatgcgc
     3121 agggtcgcca tcgattgcgt acccaacgca gacggactga tccagtggaa cggcccggcg
     3181 gccgtggccc ccggcgctgg cgcagccgtc aacgggctca cccccgagga cggccgatcg
     3241 agcatgcccg tccactgcgg cgccgaacgt ccgaactggg ccgcgaactc gggcgcggcg
     3301 tcgtcgaacc tcgcctgatc accgatacat cgatacaggt cgaacagcgt cagccaggta
     3361 tcgacatcct cgcgtcgggc accgccttca gcgaccaacg cgcgcagcag cgcctcggcg
     3421 ccggcgccat cgccattggc aaacaggatc gcggcctcct cgatctcagg atcctggcgg
     3481 ccggcggcca tcacttcaac gttgaagtcc tgcacctcgc ccagccaggg gatggcgcgc
     3541 gcagcggctg cccctcccgc tgatggatgc agtggctggg cggacaaaga ctccttggcc
     3601 aaggctgacg ccgacccgtc tgccgcggcg tccagcagcg gcgcgccacc ctccggcggc
     3661 ggtggcagtg gcatcgggtc ggtcttgtcg taggcgtgga ccgacggctc cgatatggca
     3721 tccgccgccg gcccggcagc ttgattcttc cccactcgat gcctggacca tgcggtcgac
     3781 atctgcgcct cgatctcgtc gatcttcttg agcgtgagct cccgttcgcc ggtattggcg
     3841 ggatggctgc tggggtaaaa tgaagggggg cccgcggcaa ggtcgctgcc cttcagcgtc
     3901 tcgcggcggc gcagcttgcg cagcatgtcg aactcgcgac tgcgcacaaa atcgttgcga
     3961 cgtttgcgct cgatcatctc cttgagcgcc agccgcgact ggctgtcgcc atcctcggaa
     4021 tcgggctgat cgagatccgc ccagtgcgtg gtggggttct tgacgaaacg taccaccttc
     4081 gaaagcagtc cacccggctc ggtaggcttg ttcatggctc ggtacctccc gtggtcagtg
     4141 catgccctgc ggcggcgcga gattcagaga cgaagcatgc ggtcgcagca gcgtcggcga
     4201 gcctgcggtg gccgcaccgc acggcaccac catggatcgc accacgaccg gcgaccgcct
     4261 gcgcggtgcg cggcgctgca gcacggccgt cgggcatgaa cccatcgtct cgcctcggat
     4321 cagtcgccga acattttctg tttcagctca cgccgctgct gggcctccag cgacagcgtg
     4381 gcggtcgggc gcgccagcag gcgcggcacg ccgatcggct cgccggtttc gtcgcagtag
     4441 ccgtagtcac cggcatcgat gcgggcgatc gattgctcga tcttcttgag cagcttgcgc
     4501 tcgcggtcgc gcgtgcgcaa ctccagcgcg tgctcttcct cgatggtggc gcggtcggcc
     4561 gggtcgggca ccaccaccgt gtcgtcgcgc aggttctcgg cggtctgatc cgcgttcgcc
     4621 agcatgtctt ccttgagctg gaccagcttc aggcgaaagt aggccagctg cttgtcgttc
     4681 atgtactcgt tgtccggcat cgcaatgatt tccggatcac tgagctgttc gaccgacttg
     4741 gtcttccagt tgttggccag cttggggtct ttcttgaccg gcgcgggcat cgggagggtg
     4801 gcaacggagg gcatgggcag attcctggat accggctgcg gcggcttggt cggcacggcg
     4861 ggcgacgcct tggcggcggc cgagcggacc ggtgacttct tgctcggctc aggggcggac
     4921 acccgggcct tgctggccgg cttgccggcg ctcgaaggtg ccttggcggg tttggccttg
     4981 ggggttgccg ggctcttggc agccgcagtt ttcacttgtc tcgtctcctg ccggcaccga
     5041 cctccagggt ggcgccgacg acatggccag cagatgaagc cgggtctgcc tcatccgatc
     5101 agcgccctgt tgtttgacct tcttgggcga tgcgggcaca gcgcccccgt tcaccggggt
     5161 tgcttacgaa ggcggcgatt gtagccacgc ccggccggcc tgggcacacc gggcaaacaa
     5221 atcgtcgacc ttgtgcgaaa accttgacgc aagccccaat cggatggtcc ggcggttcgg
     5281 caggcacgca ccgaatcccg catgccgaaa acgcgcaccg caacgcgacc ggtcaaggcg
     5341 cgagccccgg cggcaacacc ggccacggcg agcatctgcc gtgcggcgac ggagcgggtg
     5401 ggcacaccgg gcgggcatga aagacccaac cacccctgtt caggcggaga ccagacactg
     5461 ctccaggccc tgggtgaaga tgtcgcgcgg caggtcgatc ccgatgaaca ccatcttgct
     5521 ctggcgcggc tcgtcgccct tccaggccgg ccccagatcg ctgcccatga gctggtgcac
     5581 gccctgaaag atcaccttgc gctcggtgcc cttcatccac agcacgccct tgtagcgcag
     5641 catgcgcggg ccgtagacgt tgacgatggc gcccagaaag tcctccagcc gcgccgggtc
     5701 gaaggcacgc tcggcgcgga acacgaagct cttgacgtcg tcgtccgcgt ggtggtggtg
     5761 gccgtgcgcg tgctggtgcg acgggtggtc gcagtggcca tcgtggtggt gatggtggtc
     5821 gtgatgatcg tggtcatggt cgtcggccgc cagaaattcg gggtcgatgt ccagtcgggc
     5881 gttcaggttg aaaccgcgca gatcgaacac ctcggcgatc ggcaccgcgc cgaaatgcac
     5941 caccttctgc gccgcgcgcg ggttcatgtg cttcaggcgg tgctgcaggg ccgccagctc
     6001 gcctgcatcc accagatcgg ccttgctgat gaagatctgg tccgcaaagc cgacctggcg
     6061 ctgcgcctcg atgcggtcgt tcagctgctg cgccgcgtgc ttggcatcga ccagggtgac
     6121 gatggcgtcg agcaggtagc cttccgcgat ctcgtcgtcc atgaagaagg tctgggcgac
     6181 cggaccgggg tcggccaggc cggtggtttc gatcaccacg cgctcgaagt cgagctcccc
     6241 gctgcgcttt ctgcgcgcca gatcggacag ggtggcgcgc agatcctcgc ggatgctgca
     6301 gcagatgcag ccgttgctca tctggatgat ctgctcctgg ctgtcgctga ccaggatgtc
     6361 gttgtcgatg ttctcctcgc cgaactcgtt ctcgatcacg gcgatcttct ggccgtgcgc
     6421 ctcggtcagc acgcgcttga gcaaggtggt cttgccggag ccgagaaagc cggtaaggat
     6481 ggtggcggga atcagtgaca tggcagggtg cgggcgtggc gcccgccgat gggagaagac
     6541 gtcgaacacg gcagtgtagc cacccccatc caccgcggct gcggctgcgg cccgcatgcc
     6601 caagtgttgc ccgaatccac gatcagaagc tccgccaccg gaaaagcgca agtccaacga
     6661 gctgtcaatc gtaaacctta gttgtatttt tttgtagatt gcggttcaat ggtgccgaag
     6721 cttcggtctg caacgcggtc aacgcgcacc gtggccaccg cttgctggga ccccaagcga
     6781 cttctccact caccgtgcat ccatgtccaa gtctttcaac cttgtggccc tcgccctcag
     6841 tctgggcatc accggcgctc aggcgcgcga gctcgtgctg ggcatcagtg aaggcacctc
     6901 gggcggcacc gaccacgccc gagtcgtgca gaagtacggc ggtctggccg aagccatagg
     6961 taaggccatc aaggccgagg tgaaggtggt gttcgtgcgc gagtttgcac agttggagga
     7021 tgggatgaag agcgcccgcc tcgacctggt gatggcgcgc ccgagcgact accctgcgcg
     7081 cggcttgcgc gactacggct atcactatgt ggccacggtg aaacccgaag gacagtgcct
     7141 gatcgtcgtg cccaaggatt cgcccatcaa acagctggca gacctcaaag gcaagaagat
     7201 cgtcatgcca gagaaggtcg cctatatgac caaattctgc aaagccgagt tgcgccacca
     7261 gggcgtcgac ctcgacaagg agagcgtcac ctacgtgcgc gaacaggagg ccgtggtgtt
     7321 ctacatcaac aacggtttcg ggcaggtggg tgggctggca tcctattcgg gggcggcgcg
     7381 caaatggctg aaggaaggtg gcaccgtgct gcaccgcagc gtgccgcagc cctattcgcc
     7441 actgattgcc tccaaggcgt tgagtgcggc cgacattgcc gccgtgcaga aagcggtgct
     7501 ggccttgcct gacagcccgg aaggccaggt ggtgctgaaa tccatcggca tgccgggcgg
     7561 ctttgacacc gccaccgaag acaagttgcg cggactgctc aagtttctgg gcgactgatc
     7621 acggcctgcg cggccccgtt gctcagcgcc gtcgcgcgcg cgggtgggcg gcgtcgtagg
     7681 cctgcgccag gtgctgaaaa tccagtcggg tatagacctg cgtggtgccg atgctggcat
     7741 ggcccagcag ttcctgcacg gcgcgcagat cgccgctgga ctgcagcaca tggctggcga
     7801 aggagtggcg cagcatgtgc ggatgcaccg gcgtggccag ccccgcctgc tggctgcgcc
     7861 ggcgcaggcg ctgccagacc gatggcgcgc tcagacgggt gccgcgccgg ccgatgaaca
     7921 gcgccgccgc ggcctcgccc gcggccatca cctggccgcg caccgccagc cagcgccgca
     7981 gggcttcggc ggccgagcgt cccaggggca cggcgcgccg cttgctgccc ttgccctgca
     8041 cctgcacctc ggcctggtcc agatcgatcc agccacggcc agcgcgtgcg gcggcgtcgc
     8101 tggccgccac gtccagcccc accagctcgc ccacgcgcag accgctgccg tacagcagct
     8161 ccaccatggc ggcgtcgcgc gcgtccagcc aggggtcatc gccctcgtcg atgtgttcgg
     8221 ccagttgcac cgcctcgtcc acccccagcg ccttgggcag cggccgcggc tggcgtggcg
     8281 cccggatgcc cagcaccggg ttggcaccga tccctccttc gcgccccagc cagacgtaaa
     8341 agccccgcca gcccgacagg atcaacgcaa tgccgcgccc gctgcgaccg ccggcatgca
     8401 tgcgcgccac ccaggagcgc acatggtggt gctgcacctg gggcagcgcc acaccggcct
     8461 gcgcggccag gcgggcaagc ttgtccagat ccgctcgata cagcgcgagc gtgcgtgggg
     8521 ccaggcgctt gagcacccgc gcatgggtga ggtagcgttc gaccagcgcg gcgtcggccg
     8581 cggcggcgga cgggaggggt tcgacaaccg atgacatgct atttgaatga tagctttttg
     8641 cgcagtcgga tcaagcacca gagcccacat tcaacctgga aacggcagtt gaccgcttgt
     8701 ccgctgcggg cagaccgcat cgatgcgggg tctggcggct gcgatggcct tcacctcttg
     8761 cgtgagcacg ctacgcaccc acccgcagcg catcgacgcc cggttgccca acccgaccgg
     8821 gcgccgcccc ggcgcggcgg cgctgctagc atcgcaagca tgaccgccca cgacgaccgc
     8881 gtcaccgacg tgcagcccaa gcgcgaaacg ccgcgccccc cggcgcacgc cccggtgccg
     8941 cgtgccgagg ccaccgccgc cgcacgcccc gacgcccgat tcatggccgc gcacccggcg
     9001 cacgcgctgg cgctggggtt cggcagcggc ctgtcgcccg tggctcccgg cacggccggc
     9061 acgctgtggg cctggctggc gtggtgggca ctgggcctgt ggctgagccc ggcgcagcag
     9121 ggctggctgc tggtcggcgc gctgccgctg gcctggtggg cctgcaccgt caccgcgcaa
     9181 cacctgcaga cacccgaccc gggtgccatc gtcatcgatg aggtggtggc gttctggctg
     9241 gtgctgtggc tgctgctgcc ggcggggttc tggggccagg ccacggcctt tgcgctgttt
     9301 cgcttcttcg acgccgtcaa gccgggaccg gtgggctggg ccgaccgcct gctgcacgga
     9361 cgccgcggct gggcgggcgg cttcggcgtc ctgctggacg acctggtggc cgccttctgc
     9421 accttgctgg tgctggcact gtggcgcccg gggtgagcac cgatgctacc aaaacaagag
     9481 ctttcggcac ttactggatc tgcgccagag gccaaaatcg ttcatgacat cgcagccctg
     9541 ctgaccgccc gcggctggtt gctgacgacc gccgagagct gcaccggcgg cctggtggct
     9601 gccgcctgca ccgagctggc cggctcctcg ctgtggttcg aacgcggcat cgtcagctac
     9661 agcaacgccg ccaagagcga gttgctgggc gtgccgccgg cactgatcgc gcgccatggc
     9721 gcggtcagcg agccggtggt gcgcgccatg gccgaaggcg ccctggcgca cgcgcgcgcg
     9781 caggtcagcg tcgccctcac cggcatcgcc gggccgaccg gtggcagcgc cgacaagccg
     9841 gtcggcaccg tgtggttcgg ctggtgtgtg gacggacgca ccgacaccca gcggtgccgg
     9901 tttgacggcg accggcaggc cgtgcgcgtg caggccgccc tgcacgccct gcgcgggctg
     9961 gccgcccgcc tggcggcgcc tggtggcgcc gcgaccccgc tcacttgacg gcgatcaggc
    10021 gccagtcgag ccggttgtcg gcgcggtgca cgacctcgat gttcttcttc atcgcccagg
    10081 gaatgacctg gttgtgcagc ggcaggtgcg acacgtcctt gtgctccagg atcagggcct
    10141 gctcgatcag ctcggcgcgg cgcttctgat cgacctcgac cttgacgcgc tcgaccagcg
    10201 cgtccatctg cgggttggag tagcgcccca cgttgtagtt gccgtcgccg cccgcaccca
    10261 cgctgcgcac cagcgactgc agactgtaga gcgcatcgaa ggtcggcacg ccccagccca
    10321 gcatgtagat gctggcctcg tggcgctgga tcatcgggaa gtagctcacc agcggcatgg
    10381 tgcggagctt ggccttgaca cccaccttgg accacatcgc ggccaccgcc tggcagatgc
    10441 gctcgtcggc gatgtagcgg ttgttggggc aggcgaaatc gacctcgaag ccgtccttgt
    10501 aaccggcctc ggccagcagc ttctgggccg ccgccgcgtc gtacggatag cgctggtcgg
    10561 cgcgcttggt gtagccgctc acctgcggcg cgatcagcgt gccggtgttc tggctcaggc
    10621 cgcgcatcac caccttgtgg atggcgttga tgtcgatggc ctgatacagc gcctggcgca
    10681 cgcgctggtc tttcaggggg ttcttgccct tgacgctgga gcccggcagt tcgtcgcggt
    10741 gctggtccat gccgaagaag atggtgcggt tctccggccc ctgcaccacc ttgagggccg
    10801 cgttgcgctg cacgcgctcc agatcctgcg gcgtcgggtc gagcacgaag tccacctcac
    10861 ccgacagcag cgccgccatg cgcgtggcct cggacttgat cggggtgtag atgacctcgg
    10921 tgacgttgcc cggcttggcc ttggcgccat gcccccacca ctgggggttg tccaccagca
    10981 ccagcttctg gtcgggctgc cattccttga ccatgaaggg gcccgtgccc atggcgttgc
    11041 ggtgggcaaa gctctcgtcc ttggtcttga tgtccttggg ctcgaccgac ttgttcttct
    11101 cggcccaggc cttgctcatg acgcgcagtt cggtgagctg attgagcagc accgggttgg
    11161 gcgccttggt gaagatgtcc agcgtgttcg gccccgccac caccaccttg tcgatgccct
    11221 gcgtgtagac gctgtagttg gaggtcttgg ccatggcgcg ctcgatggag aacttgacgt
    11281 cctcggcggt catgctgctg ccgtcgctga acttcacgcc ggtgcgcagc gtgaactgca
    11341 tctgggtcgg actgacctgc ttccaggccg tggccagcat gggctcgatc ttgaagctct
    11401 tgctgttgta gtagaccagc gactcataga cggcggcgtg cacgccgttg cccagcgcgt
    11461 tgttctgcga atggatgtcc agcgtcggaa tgtcgctggc gctggtccat ttgaaggtcc
    11521 tggcgtgcgc acccagggtg cagcagacgg ccagtgcggc cacggcgagc gtgcgaagct
    11581 tcatatcgga tgtcctccag ggtgatgggc aaagtcgcgc agtatgccgc gccgccgcca
    11641 gaccacccac agggaagtcc ctgaagcgcg ccggcggccc ggctcgcacc gccgcatgaa
    11701 cccggcgcgc ggcgaggaaa accctaatcg gtcggccgcc gccccacccc cataatgccg
    11761 cgttcccgga acttgaacaa agcagtcgaa gacatgagtt cggacatcat tctggaaacc
    11821 cgtgggctgg tgaaggagtt caagggtttc gtcgccgtca acgacgtcaa ccttcaggtg
    11881 cgcaagggca ccatccacgc actgatcggc cccaacggtg ccggcaagac cacctgcttc
    11941 aacctgctga ccaagttcct ggagcccacg cgcg
//
LOCUS       DAOOOG010000068        11853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6001_length_11853_cov_26.065265, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000068 DAOOOG010000000
VERSION     DAOOOG010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11853
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6001_length_11853_cov_26.065265"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PK242_06735"
     CDS             <1..245
                     /locus_tag="PK242_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ93751.1"
                     /translation="AMQAGLRDVVKDILDSLTPREAKVLRMRFGIEMTSDHTLEEVGK
                     QFDVTRERIRQIEAKALRKLKHPSRSDKLRSFIDSL"
     gene            326..1543
                     /locus_tag="PK242_06740"
     CDS             326..1543
                     /locus_tag="PK242_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HOZ93752.1"
                     /translation="MHPDAARLWTAPRWALAILLALLGMLGPFSIDTYIPAFAAIGQS
                     LQATPVQMQQTLSAYLLGFAFMNLFHGALSDSFGRRPVILWGLALFALASVGCALSQS
                     VGQLIFFRALQGFSTGAGIVVSRAVIRDMFPPHEAQRVMSQVTIFFGVAPAVAPMIGG
                     LIAQYLNWHAIFWFLVAVGVLLWLANWRLLPETLHASHRQPFQVGHLMRGYGQLLSDP
                     RFMLLALASGVPFNGLFLYVLSAPAFLGEILQLAPTQFFWFFVLNISGIMAGSWLSGR
                     KAGKVSPKNQILQGFRIMLAVSLINLVANALFDPHPLWSMWPMTVFAFGWALMVPVVT
                     LLVLDLHPDRRGMASSLQAVVGSTANGIVAGAVAPLVMHSALGLAVMSAAFMLVGLVA
                     WLWLRRRWPEIGG"
     gene            1899..3473
                     /locus_tag="PK242_06745"
     CDS             1899..3473
                     /locus_tag="PK242_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOZ93753.1"
                     /translation="MHQSSNPKAPPMTEAEFTRARQIYFERCAGCHGVLRKGATGKAL
                     TPDLTVGKGTEYLKVFIAYGSPAGMPNWLTSGEMDEKTVDLMARYIQHDAPTPPEFSL
                     EDMEKSRKEYVPVAQRPKKKMNNYNINNIMSVTLRDSGEVALIDGDTKQIINIVKTGY
                     AVHISRLSASGRYLYVIGRDARLNLIDLWMEKPDNVAEIKVGLEARSVETSKFKGWED
                     KIAIAGTYWPPQFVLMDGDTLKPRKVVGTRGMDINNEYHPEPRVAAIVASHQKPEFLV
                     NAKETGKIWMVDYTDLKNLKTTQIDTAKFLHDGGFDATKRYFLTAANASDKIVVVDTK
                     TSKLEAIVDVGKTPHPGRGANFKHPKFGPVWATSGLGDERIDIIGTDPAKNKQHAWKV
                     VQTLKGQGGGSLFIKTHPKSTNLWVDTALNPDPKISQSVAVFDVRDLSKPPQVIDIAA
                     CANLKDDGAKRVVQPEYNQKGDEVWFSVWSAKNKESAVVVVDDKTRTCKTAIKDPKLI
                     TPTGKFNVWNTQHDVY"
     gene            3553..3897
                     /locus_tag="PK242_06750"
     CDS             3553..3897
                     /locus_tag="PK242_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ93754.1"
                     /translation="MSRRPLLLFVLSLAWLPAWAPAAEPGPERQAQLIRMVRQDCGSC
                     HGIRLTGGLGPALSRERMLDIPFDSLLATIYNGRPGTPMPGWKSMISEADATWIAHQL
                     QAGFPLESGPSR"
     gene            3894..5087
                     /locus_tag="PK242_06755"
     CDS             3894..5087
                     /locus_tag="PK242_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOZ93755.1"
                     /translation="MTFPTRRAVLGLAFSTSLLAGCAPALLVPPERGGSDLGLVIERE
                     SGSIGVLDTSARTRLGTIDGLGDLSHASVVFARDGARAYIFGRDGALTKVNLRTGRIE
                     GRVMQAGNSIGGAISQDGTLVVAQNYQPGGIKVFDARTLAPLADIPALVDGKASRVVG
                     LADLPGQRFIFSLFDAGQIWIADLSQPTRPVLTKFEQIGKQPYDALVSPNGRHYIAGL
                     FGEDGLAMIDLWAPQPRVQRILPGYGKGEQPLPVFKMPHLRGWSIAGRHAYLPAIGRR
                     EVLVVDTDSWQEVGRIPVAGQPVFVMARPDGRQVWVNFAVPDYDTVQVIDTQTRQVVH
                     SLKPGKAVLHMEFTPRGDTVWISARDDHHVSVIDTASFRTLAVLPAKSPSGIFFASRA
                     ARTGF"
     gene            5182..6156
                     /locus_tag="PK242_06760"
     CDS             5182..6156
                     /locus_tag="PK242_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOZ93756.1"
                     /translation="MQARLLNEWQRGFPLSDRPFAQIGQALGATESEVIQACAELSRL
                     GIISRIGGVWAPGVGGSALLCAMAVPPARLEAVAARVSAHPGVNHNYEREHALNLWFV
                     ITGHDATQTEQALAAIESDTGLPVLRLRMVRPYRIDLGFDLHRPARAGTALRQRPPRV
                     AETDIPLAACVEQGLPLLPRPYDLWAARLGSSPAQLHAQLQAWLDAGTLRRFGLVVRH
                     HEVGFAANAMTVFDIEDAQVDALGERLATQPGVTLCYRRERAQGWPYNLYCMVHGRNR
                     EQTLALLNAAIAGAGLGERPRQVLFSRRRFKQTGARYFRPLEAPADGR"
     gene            6146..6547
                     /locus_tag="PK242_06765"
                     /pseudo
     CDS             6146..6547
                     /locus_tag="PK242_06765"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
     gene            6689..7174
                     /locus_tag="PK242_06770"
     CDS             6689..7174
                     /locus_tag="PK242_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOZ93757.1"
                     /translation="MVLTEFDRALIAATQSGLPLVPRPYDAVAAMLGSSSAAVRERLA
                     ALLAAGVVRRIAAVPNHYRLGYTANGMSVWDVDDAEVDRLGELLGSQPGVSHCYRRPR
                     KPGVWRYNLFAMLHGHSREQVLAQAEQVAALLGPACRAHDVLFSSAILKKTGLRLRQN
                     V"
     gene            7217..8806
                     /gene="nadB"
                     /locus_tag="PK242_06775"
     CDS             7217..8806
                     /gene="nadB"
                     /locus_tag="PK242_06775"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOZ93758.1"
                     /translation="MATLEFDVLIVGSGLAGLSAALHLAPTRRVAVITKRELQEGSSR
                     WAQGGIAAVLANDDSFAAHIQDTQVAGAGLCDLATTRFVVENAPAAIGWLRGLGVPFT
                     LEGEELHLTREGGHSARRIVHATDATGAAVQKTLIDVVRETPGIRVFEHHTLVDLITS
                     RKLGLAGQRCLGLYALDEARDEVVTFRAPQTILATGGAGKVYLYTTNPDTATGDGIAA
                     AWRAGCRVANMEFIQFHPTGLYHPQAKSFLISEAVRGEGGRLLLPPSAGGTRFMPMHD
                     ERAELAPRDVVARAIDFEMKKHGLDCVHLDISHQSPQFLREHFPNILATCASLGIDIT
                     RQPIPVVPTAHFTCGGVLTDLAGRTDLAGLYAVGEVAYTGLHGANRLASNSLLECLVF
                     ARAAAQSIDAATPAPLPALPPWDDSRVTDADESVVISHNWDELRRFMWDYVGIVRTNK
                     RLERAAHRIALLRGEIHEFYARFHVTRDLLELRNLVLVADLIVRSAQMRHESRGLHFS
                     RDWPELAAPAAPTILVPPASP"
     gene            8841..9203
                     /locus_tag="PK242_06780"
     CDS             8841..9203
                     /locus_tag="PK242_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HOZ93759.1"
                     /translation="MPHLTIEYSPNLGAFPEAQAAAELNASVCASPEVLDEADLKTRF
                     VRCAPTFQIGTVLAGRAFVHAQLRLLAGRSPEAKQDLAGRVASVLRRVTPRPDGVMVQ
                     LSVEIVDMDRPSYVKERL"
     gene            complement(9373..10482)
                     /gene="nadA"
                     /locus_tag="PK242_06785"
     CDS             complement(9373..10482)
                     /gene="nadA"
                     /locus_tag="PK242_06785"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOZ93760.1"
                     /translation="MSTTIDVEYERPEGAAACSTHVAWARVPAEPSPQERTALKARIR
                     SLLQQKNAVMVSHFYVHPDLQDLAEETGGIVSDSLEMARFGRDHAARTLVVSGVRFMG
                     ETAKILSPEKTVLMPDLGATCSLDLGCPVEEFSAFCDEHPGCTVVVYANTSAAVKARA
                     DWVVTSSCALDIVRALKDAGQKILWGPDRHLGDYIRRETGADMVFWSGACIVHDEFKA
                     LELELLKAQRPQARVLVHPESPAGVIALADAVGSTSAILRAAQEMDATEFIVATDKGM
                     MHKLRQLNPGKTFYEAPTAGNSATCKSCAHCPWMAMNSLAGVVHALETGANAIHVDPA
                     LIPRARRPIDRMLAFTAGLRQGVPAARLVPDLGAV"
     gene            complement(10479..11351)
                     /gene="nadC"
                     /locus_tag="PK242_06790"
     CDS             complement(10479..11351)
                     /gene="nadC"
                     /locus_tag="PK242_06790"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOZ93761.1"
                     /translation="MSQTTPDWMSLEAIAALARADVRRALDEDVGSGDLTAALVPAGR
                     RARARVLAREAAVICGAPWVEATLRALDPQVQLRWQVTEGQRCAADEVVLEAEGDARA
                     LLSAERTALNFLQLLSAVASKTASYVQAVAGTRAQIVDTRKTLPGLRLAQKYAVRVGG
                     GVNHRIGLHDAVLIKENHIAAAGGVTAVLRAAQAVAARAAFVEIEVETLAQLREALDA
                     GARMVLLDNMPLPMLREAVALNAGRAVLEISGGVTLDSLRALAETGVDRISVGALTKD
                     VKATDFSMRLQELP"
     gene            11484..>11853
                     /locus_tag="PK242_06795"
     CDS             11484..>11853
                     /locus_tag="PK242_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOZ93762.1"
                     /translation="MFRISQFMHELARAEATGTFPEPARRGGPPGPVVIWNLIRRCNL
                     TCKHCYAFSADHDYPGELSTSQVFEVMDDLKAFKVPALILSGGEPLLRPDIFEVAERA
                     KALKFYVGLSTNGTLIDQPLA"
BASE COUNT         1848 a         4283 c         3893 g         1829 t
ORIGIN      
        1 ccgccatgca ggccggcctg cgcgacgtgg tcaaggacat cctcgacagc ctgaccccgc
       61 gcgaggccaa ggtgctgcgc atgcgcttcg gcattgagat gaccagcgac cacacgctgg
      121 aagaagtggg caagcagttc gacgtcacgc gcgagcgcat ccgtcagatc gaggccaagg
      181 ccctgcgcaa gctcaagcac cccagccgca gcgacaagct gcgcagcttc atcgattcgc
      241 tgtaagccga ttcaacgata aaacctacgc tggccggcgc ccagactgcg cggccagctt
      301 ctttttttat agcatactgg ttcccatgca ccccgacgcc gcccgactct ggacagcccc
      361 gcgctgggcc ctggccattc tgctggcgct gctgggcatg ctgggaccgt tctcgatcga
      421 cacctacatt ccggcgtttg ccgccatcgg ccagtcgctg caggcgacgc cggtgcagat
      481 gcagcagacc ctgagcgcct acctgctcgg cttcgccttc atgaacctgt ttcatggcgc
      541 gctgtccgac agcttcgggc gccggccggt cattctgtgg ggcctggcgc tgttcgccct
      601 ggcctcggtc ggctgcgcgc tgtcgcagag cgtcggacag ctgatcttct ttcgcgcgct
      661 gcagggcttt tccaccggcg ccggcatcgt ggtctcgcgc gcggtcatcc gcgacatgtt
      721 tccgccccac gaggcacagc gcgtgatgag ccaggtcacc atcttcttcg gcgtcgcccc
      781 ggcggtggcg ccgatgatcg gcggcctgat cgcccagtac ctgaactggc acgccatctt
      841 ctggttcctg gtcgccgtgg gcgtgctgct gtggctggcc aactggcgcc tgctgcccga
      901 aaccctgcac gccagccacc gccagccgtt ccaggtcggc cacctgatgc gcggctacgg
      961 ccagctgctc agcgatcccc gcttcatgct gctggcgctg gcctcgggcg tgcccttcaa
     1021 tggcctgttc ctctacgtgc tgtcggcgcc ggccttcctc ggcgagatcc tgcaactggc
     1081 gccgacgcag ttcttctggt tcttcgtgct gaacatcagc ggcatcatgg ccggctcctg
     1141 gctgtcgggg cgcaaggccg gcaaggtcag ccccaagaac cagatcctgc agggttttcg
     1201 gatcatgctg gcggtgtcgc tgatcaacct cgtggccaac gccctgttcg accctcaccc
     1261 gctgtggtcg atgtggccga tgacggtgtt cgccttcggc tgggcgctga tggtgccggt
     1321 agtgaccctg ctggtgctcg acctgcaccc cgatcggcgt ggcatggcgt cgtcgctgca
     1381 ggcggtggtg ggctcgaccg ccaacggcat cgtggccggc gcggtggccc cgctggtcat
     1441 gcattcggcg ctcgggctgg ccgtgatgtc ggccgccttc atgctggtcg gcctggtggc
     1501 ctggctgtgg ctgcgccggc gctggcccga gatcggcggc tgagcgagac ccgcccggtg
     1561 ccccagcggc gggcaatgca cgcaatttga accaagtcaa ggttcaaacc gggctgtgcc
     1621 tgcaagattg cgctgaccgg gagtgatatc cagcagccat cacccggccc tcgaatccgg
     1681 ctgctgcggt gacacggaac aacatgatga acaccttcaa gaggaagtcc aaactttcgc
     1741 cgctgactgc cgtactgagc acggcgctgc tgctcggtgt cagcggtgca tacgcccagg
     1801 cgggcggcga acccgcgaag ccgggcggcc ccaccacctc gccctccagc gctgaagcca
     1861 agtacgaggg cgcgccctat gcggtcgacc cttcgaccat gcaccagtcc agcaacccca
     1921 aggcgccgcc catgacggag gccgaattca cccgcgcccg ccagatctac ttcgagcgct
     1981 gcgctggttg ccacggcgtg ctgcgcaagg gcgccaccgg caaggcgctg acgcccgacc
     2041 tgaccgtcgg caaaggcacc gagtacctga aagtcttcat tgcctacggt tcgccggccg
     2101 gcatgccgaa ctggctgacc agcggcgaaa tggacgaaaa gacggtcgac ctgatggccc
     2161 gctacatcca gcacgacgcg ccgacgccgc ccgagttctc gctggaggac atggagaagt
     2221 cgcgcaagga atacgtgccg gtggcgcagc gtcccaagaa gaagatgaac aactacaaca
     2281 tcaacaacat catgtcggtc accttgcgtg attccggcga ggtggcgttg atcgacggcg
     2341 acaccaagca gatcatcaac atcgtcaaga ccggctacgc ggtgcacatc tcgcgcctgt
     2401 cggcttcggg gcgctacctg tacgtgatcg gccgcgacgc ccgcctgaac ctgatcgacc
     2461 tgtggatgga aaagcccgac aacgtggccg agatcaaggt cggtctggaa gcgcgctcgg
     2521 tcgagacatc caagttcaag ggctgggaag acaagatcgc cattgccggc acctactggc
     2581 cgccgcagtt cgtgctgatg gacggtgaca cgctcaagcc gcgcaaggtc gtcggcacgc
     2641 gtggcatgga catcaacaac gaataccacc ccgagccgcg cgtggccgcc atcgtggcca
     2701 gccaccagaa gcccgagttc ctggtcaacg ccaaggaaac cggcaagatc tggatggtcg
     2761 actacaccga tctgaagaac ctgaagacga cgcagatcga caccgccaag ttcctgcacg
     2821 acggcggctt cgacgccacc aagcgctact tcctgacggc cgccaacgcg tccgacaaga
     2881 tcgtggtggt ggacaccaag accagcaagc tggaagccat cgtggacgtg ggcaagaccc
     2941 cgcaccccgg ccgcggcgcc aacttcaaac atccgaagtt cggtccggtg tgggccacct
     3001 cgggtctggg tgacgagcgc atcgacatca tcggtaccga cccggccaag aacaagcagc
     3061 acgcctggaa ggtggtgcag acgctcaagg gccagggtgg tggttcgctg ttcatcaaga
     3121 cgcacccgaa gtcgaccaac ctgtgggtcg atacggcgct caaccccgat cccaagatca
     3181 gccagagcgt ggccgtgttc gacgtccgcg acctgagcaa gccgccgcag gtgatcgaca
     3241 tcgccgcctg cgccaacctg aaggacgacg gcgccaagcg cgtggtgcag ccggaataca
     3301 accagaaagg tgacgaagtc tggttctccg tgtggagcgc caagaacaag gaatccgccg
     3361 tggtggtggt ggacgacaag acgcgcacct gcaagacggc gatcaaggat cccaagctga
     3421 tcacgccgac cggcaagttc aacgtctgga acacccagca cgacgtgtac tgatggccta
     3481 gccccgcacc aagggccgcc gccccggcgg cccttggtgt ttgtggcgcc ccgtttttgc
     3541 tatcttcgcc cgatgtcccg acgccccctg ttgctcttcg tcttgtccct ggcctggctg
     3601 cccgcctggg cgccggccgc cgagcccgga cctgaacgcc aggcgcagct gatccgcatg
     3661 gtgcgccagg attgcggctc gtgtcatggc atccgcctga ccggcgggct ggggccggcg
     3721 ctctcccgcg aacgcatgct cgacataccc ttcgacagcc tgctcgccac catctacaac
     3781 ggccgccccg gcacccccat gccaggctgg aaaagcatga tctcggaggc cgacgccacc
     3841 tggatcgccc accagctgca ggccggtttt cctcttgaat cgggcccttc acgatgacgt
     3901 ttcccacacg ccgagcggtg ctcggcctcg ccttctccac ttccctgctg gccggctgcg
     3961 cgcccgccct gctggtgccg ccggagcgcg gcggcagcga tctggggctg gtgatcgaac
     4021 gtgaaagcgg ctccatcggc gtgctcgaca cctcggcgcg cacccgcctg ggcaccatcg
     4081 atgggctggg cgacctgtcg cacgcctcgg tggtgtttgc ccgcgacggc gcgcgcgcct
     4141 acatcttcgg gcgcgatggc gcgctcacca aggtcaacct gcgcaccggc cgcatcgaag
     4201 gccgcgtgat gcaggccggc aactcgatcg gcggcgccat ctctcaggac ggcacgctgg
     4261 tggtggcaca gaactatcag ccgggcggca tcaaggtgtt cgacgcacgc acgctggcgc
     4321 cgctggccga cattccggca ctggtcgacg gcaaggcctc gcgcgtggtt ggcctggccg
     4381 atctgccggg ccagcgcttc attttctcgc tgttcgatgc gggtcagatc tggatcgccg
     4441 acctgtcgca gcccacgcgc ccggtgttga ccaagttcga gcagatcggc aagcagccct
     4501 acgacgcgct ggtttccccg aacggccgcc actacatcgc cggcctgttc ggcgaggacg
     4561 gcctggccat gatcgatctg tgggcgccgc agccgcgcgt gcagcgcatc ctgcccggct
     4621 acggcaaggg tgaacagccg ctgccggtgt tcaagatgcc gcacctgcgc ggctggtcga
     4681 tcgccgggcg ccacgcctac ctgcccgcca tcgggcggcg cgaggtgctg gtggtggaca
     4741 ccgacagctg gcaggaggtc ggccgcatcc cggtggccgg ccagccggtg ttcgtgatgg
     4801 cgcggcccga cggccgccag gtgtgggtca acttcgccgt gcccgactac gacacggtgc
     4861 aggtcatcga cacccagacg cgccaggtgg tgcacagcct caagcccggc aaggcggtgc
     4921 tgcacatgga attcacaccg cgcggcgaca cggtgtggat cagcgcacgc gacgaccacc
     4981 atgtgtcggt catcgacacc gccagcttca gaacgctggc cgtgctgccg gccaagtcac
     5041 ccagcggcat cttctttgcc tcgcgcgccg cgcgcaccgg gttctgacgc cgtgccccgg
     5101 gttctgcccc agctgtcgca gcgcgcggac ccggccgcag cgtccgagtg gtccacgtcg
     5161 tcgtgggaca acgaccccgg catgcaggct cgcctgctga acgaatggca acgtgggttt
     5221 cccctgtctg acaggccgtt tgcgcagatc gggcaagcgc tgggcgctac ggaatctgaa
     5281 gttatccagg cgtgcgccga gctgtcacgg ctgggcatca tcagccgcat cggtggcgtc
     5341 tgggcaccgg gggtgggcgg cagcgcgctg ctgtgcgcca tggccgtgcc gcccgctcgg
     5401 ctcgaagcgg tcgccgcccg ggtttcggcc cacccggggg tgaaccacaa ctacgaacgc
     5461 gagcatgcgc tcaacctctg gttcgtgatc accggccacg acgcgacgca gaccgaacag
     5521 gcgctggcag ccatcgaatc cgacaccggg ctgccggtgc tgcgcctgcg catggtgcgg
     5581 ccctaccgca tcgacctcgg cttcgacctg caccgcccgg cgcgcgccgg caccgcactg
     5641 cggcagcgcc cgccccgcgt cgccgagacc gacatccccc tggccgcctg cgtcgagcag
     5701 ggcctgccgc tgctgccgcg gccctacgac ctctgggccg cacgcctggg cagcagcccc
     5761 gcgcaactgc atgcgcagct gcaggcgtgg ctggacgccg gcacgctgcg ccgcttcggc
     5821 ctggtggtac gccaccacga ggtcggcttt gccgccaacg ccatgacggt gttcgatatc
     5881 gaggacgcgc aggtcgatgc cctgggcgag cgcctggcca cgcagcctgg cgtcaccctg
     5941 tgctaccggc gcgagcgcgc ccagggctgg ccctacaacc tgtactgcat ggtgcacggc
     6001 cgcaaccgcg agcagaccct ggcgctgctg aacgccgcca tcgccggcgc cggcctgggc
     6061 gaacggccgc gccaggtgct gttcagccgc cgccgcttca agcagaccgg ggcccgctat
     6121 ttccgcccgc tggaagcacc cgccgatggc cgctgacacg caccgcgacg cgccacgtct
     6181 gccggccgac atcgacctgc aggacgcccg cctgatcgat cgcctgcaac gcagcgtgcc
     6241 actgaccgag cgcccgttct ccgatctggg caccgaactg ggcctgagcg aggcggcggt
     6301 gctcgatcgc ctgacgcagc tgctcggttc gggcgtgctg acgcgcctgg gcccgctcta
     6361 ccagatcgag cgcgccggcg gccagttcat cctggcggcg ctgcaggtgc cgcagcagcg
     6421 ctacgccgaa gtcacggccc aggtcaacgc cctgcccgag gtggcccaca actatcgtcg
     6481 cgagcacgcg ctcaacatgt ggttcgtggt tgccgccgag accccggccg tcgcctgggc
     6541 cacctgcgac cagatcgaag ccgccaccgg cctgccggtg catgccttcc cgaaggagcg
     6601 cgaatacttc gtcggcctgt acctgccgct gctggaccgc cctgacccgg cgccgcccgc
     6661 cgcgcaggcg gcgccggctg cgaccgcgct ggtgctgacc gaattcgatc gcgcgctgat
     6721 cgccgccacc cagtccggcc tgccgctggt gccacgtccc tacgacgccg tggcggccat
     6781 gctgggcagc agcagcgcgg cggtgcgcga gcgcctggcg gcgctgctgg ccgccggcgt
     6841 ggtgcgccgc atcgccgccg tgcccaacca ctatcgcctg ggctacaccg ccaatggcat
     6901 gagcgtgtgg gacgtcgacg acgccgaggt cgatcgcctg ggcgagctgc tgggcagcca
     6961 gccgggcgtc agtcactgct accgccgccc gcgcaagccc ggcgtgtggc gctacaacct
     7021 gtttgccatg ctgcacggcc actcgcgcga acaggtgctg gcccaggccg agcaggtggc
     7081 cgccctgctc gggcctgcct gccgcgcgca cgacgtgctg ttttccagcg ccatcctcaa
     7141 gaaaaccggt ctgcggctgc gccagaacgt ctgaagcccg gctgcggccg gcgcagtcga
     7201 ccgattacca tgcgccatgg ccacactgga attcgacgta ctgatcgtgg gcagcggcct
     7261 ggccggcctg tcggccgcct tgcacctggc gcccacgcgg cgtgtggcgg tgatcaccaa
     7321 gcgcgagctg caggaaggct ccagccgctg ggcgcaaggc ggcatcgccg ccgtgctggc
     7381 caacgacgac agttttgccg cccacattca ggacacccag gtggccggcg ccggcctgtg
     7441 cgatctggcc accacccgct tcgtggtcga gaacgccccc gccgccatcg gctggctgcg
     7501 cggcctgggc gtgccgttca cgctcgaagg cgaggagctg cacctgacgc gcgaaggcgg
     7561 ccacagcgcg cggcgcatcg tgcacgccac cgacgccacc ggcgccgcgg tgcagaaaac
     7621 gctgatcgac gtggtgcgcg aaacgcccgg catccgcgtg ttcgagcacc acaccctggt
     7681 ggacctgatc accagccgca agctgggcct ggccggccag cgctgcctgg gcctgtacgc
     7741 gctcgacgag gcgcgcgacg aggtggtgac cttccgcgcc ccgcaaacca tcctggccac
     7801 cggcggcgcg ggcaaggtct acctctacac caccaacccc gacaccgcca ccggcgacgg
     7861 catcgccgcc gcctggcgcg ccggctgccg ggtggcgaac atggaattca tccagttcca
     7921 tcccaccggc ctgtaccacc cgcaagccaa gtcctttctg atcagcgagg cggtgcgcgg
     7981 cgaaggcggg cgcctgctgc tgcccccctc ggccgggggt acgcgcttca tgcccatgca
     8041 cgacgagcgc gccgagctgg cgccgcgcga cgtggtggcg cgcgccatcg atttcgaaat
     8101 gaaaaagcac ggcctggact gcgtgcacct ggacatctcg caccagagcc cgcagttcct
     8161 gcgcgaacac ttccccaaca tcctggccac ctgcgccagc ctgggcatcg acatcacccg
     8221 gcagcccatc ccggtggtgc ccacggcgca cttcacctgc ggcggcgtgc tcaccgacct
     8281 ggccggccgc accgacctgg ccggcctgta cgccgtcggc gaggtggcct acaccggcct
     8341 gcacggcgcc aaccggctgg ccagcaactc gctgctggaa tgcctggtgt ttgcgcgcgc
     8401 ggccgcgcag tcgatcgacg ccgccacgcc ggcgcccctg cctgccctgc cgccctggga
     8461 cgacagccgc gtgacggacg ccgacgaatc ggtcgtcatc tcgcacaact gggacgagct
     8521 gcgccgcttc atgtgggact acgtgggcat cgtgcgcacc aacaagcgcc tggagcgcgc
     8581 ggcgcaccgc atcgcgctgc tgcgcggcga gatccacgag ttctacgccc gctttcacgt
     8641 cacccgcgac ctgctggagc tgcgcaacct ggtgctggtg gccgacctga tcgtgcgcag
     8701 cgcccagatg cgccacgaaa gccgcggcct gcacttcagc cgcgactggc ccgagctggc
     8761 cgcaccggcg gcgcccacca tcctggtgcc gccggcttca ccctgagctg gacgctcgtc
     8821 aaccaccgga gcgctgtccc atgccccacc tgaccatcga atacagcccc aacctcggcg
     8881 ccttccccga agcccaggcc gccgccgagc tcaacgcaag cgtctgcgcc agtcccgaag
     8941 tgctggacga ggccgacctc aagacccgct tcgtgcgctg cgcacccacg tttcagatcg
     9001 gcaccgtgct ggccggccgc gccttcgtgc atgcccagct gcgcctgctg gccggccgca
     9061 gccccgaggc caagcaggac ctggcaggcc gcgtggccag cgtgctgcgc cgcgtcaccc
     9121 cgcgcccgga cggcgtgatg gtgcagctga gcgtggagat cgtcgacatg gaccggccgt
     9181 cctacgtcaa ggagcggctc tgacgggatc tcgccctcag gacgacagat gaccgacaac
     9241 ctcgctgcgc gtcgatgaca aagaaggcat cggtcatgcc ggcgcagggg gttcggccgg
     9301 cctcgatcag gacggttaaa aacggctgca gcgcagaccc agaaagcgcc agcagctatg
     9361 ttttagatag catcagacgg cacccagatc gggcaccagg cgtgccgccg ggacgccctg
     9421 gcgcaggcct gcggtgaacg ccagcatgcg gtcgatgggc cggcgggcgc gcgggatcag
     9481 ggccggatcg acatggatgg cgttggcgcc ggtttccagc gcatgcacca cgccggccag
     9541 gctgttcatg gccatccatg ggcagtgggc gcagctcttg caggtggcgc tgttgccggc
     9601 ggtgggggcc tcgtaaaacg tcttgccggg gttgagctgg cgcagcttgt gcatcatgcc
     9661 cttgtcggtg gcgacgatga attcggtggc gtccatctcc tgcgccgcgc gcaggatggc
     9721 gctggtggaa cccacggcgt cggccagggc gatcacgccg gccgggcttt cagggtgcac
     9781 cagcaccctg gcctgcgggc gctgcgcctt cagcagctcc agctccagcg ccttgaattc
     9841 gtcgtgcacg atgcaggcgc cgctccagaa caccatgtcg gcgccggtct cgcgccggat
     9901 gtagtcgccc aggtggcggt ccggccccca caggatcttc tggcccgcgt ccttgagcgc
     9961 gcgcacgatg tcgagcgcgc agctcgaggt caccacccag tcggcgcgcg ccttcacggc
    10021 cgcgctggtg ttggcgtaaa ccaccaccgt gcacccgggg tgctcgtcac agaaggcgct
    10081 gaactcctcc accgggcagc ccaggtcgag cgagcaggtg gcgcccaggt cgggcatgag
    10141 cacggttttt tcggggctca ggatcttggc cgtctcgccc atgaagcgca cgccgctgac
    10201 caccagcgtg cgcgccgcgt gatcgcgccc gaagcgcgcc atctccagcg agtcgctgac
    10261 gatgccgccg gtctcttcgg ccaggtcctg caggtcgggg tgcacgtaga agtgcgacac
    10321 catgacggcg ttcttttgct gcagcaggct gcggatgcgc gccttcagcg cggtgcgctc
    10381 ctgcggcgac ggttcggccg gcacgcgcgc ccaggcgacg tgggtggaac acgcggccgc
    10441 gccttcgggg cgctcgtatt cgacgtcgat ggtggtgctc atggcagctc ctgcagacgc
    10501 atggaaaagt ccgtggcctt cacgtccttg gtcaatgctc cgacggagat gcggtcgacc
    10561 ccggtttccg caagggcacg caggctgtcc agggtgacgc cgccggagat ttcgagcacc
    10621 gcgcgcccgg cgttcagggc caccgcctcg cgcagcatgg gcagcggcat gttgtccagc
    10681 agcaccatgc gggcgccggc gtccagcgcc tcgcgcagct gcgccagcgt ctcgacctcg
    10741 atctcgacaa acgccgcccg cgccgccacc gcctgtgcgg cgcgcaacac ggcggtcacc
    10801 ccgccggcgg cggcaatgtg gttttccttg atcagcacgg cgtcgtgcag gccgatgcgg
    10861 tggttcacgc caccgcccac gcgcacggcg tacttctgcg ccaggcgcag gccgggcagg
    10921 gttttgcggg tgtcgacgat ctgcgcgcgc gtgcccgcga ccgcctgcac gtagctggcg
    10981 gtcttgctgg ccaccgccga cagcagctgc aggaagttga gcgcggtgcg ctcggccgac
    11041 agcagcgcgc gcgcatcgcc ttcggcctcc agcaccacct catcggcggc gcagcgctga
    11101 ccttcggtca cctgccagcg cagctgcacc tgcgggtcga gcgcgcgcag cgtcgcctcg
    11161 acccagggcg cgccgcagat cacggcggcc tcgcgcgcca gcacgcgggc gcgggcgcgg
    11221 cggcccgccg gcaccagggc ggcggtcagg tcgccgctgc ccacgtcttc gtccagtgcg
    11281 cgacgcacgt cggcacgcgc cagggcggcg atcgcctcga ggctcatcca gtcaggggtg
    11341 gtttggctca tgggagacga agcatagcgc ctggcccgcg ccagcgtctg ccggcccgcg
    11401 gcaccggctg gaattgacac atgtcatggc caacgccggc aggctgccta tcatcgaccc
    11461 tggctgttct ggaaggtttc ctcatgttcc gcatctccca attcatgcac gagctggcgc
    11521 gcgccgaggc caccggcacc ttccccgagc cggcgcgccg cggcggcccg cccggcccgg
    11581 tggtgatctg gaacctgatc cgccgctgca acctgacctg caagcactgc tacgccttca
    11641 gcgccgacca cgactacccc ggcgagctga gcacgtcgca ggtgttcgag gtcatggacg
    11701 atctcaaggc cttcaaggtg ccggcgctca tcctgtcggg tggcgagccg ctgctgcggc
    11761 ccgacatctt cgaggtggcc gaacgcgcca aggcgctcaa gttctacgtc ggcctgtcga
    11821 ccaacggcac gctgatcgac cagccgctgg ccg
//
LOCUS       DAOOOG010000069        11758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6086_length_11758_cov_35.291720, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000069 DAOOOG010000000
VERSION     DAOOOG010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11758
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6086_length_11758_cov_35.291720"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1401
                     /locus_tag="PK242_06800"
     CDS             join(119..455,455..1401)
                     /locus_tag="PK242_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOZ93763.1"
                     /translation="MSTTRHTDTVEVILKDQRRRRWSPAEKAALVRRTYEPGMSVSLV
                     ARQEGVAASLLFQWRKLDRQGALTAVSAGEAVVPASELAAARAEIAKLQRVLGKKTLE
                     NEILKEAVEYGRRKKVDCALALVARGRPMKTVCRALGVARSHVHELHHRSPDWRDGRK
                     GRTPAGDAQLLAEIRERIAELPSYGYRRACALVNRQRSTQGDARVNPKRAYRVMALAG
                     LLLPKAPRRRQSSRTHEGTVAVGRSDLRWCSDGLEIKCDSGQTVTTTFAKDCCDREVI
                     AWRAWEGKGLPGEPVREMLIEAVEKRFGSVEAVPPDHWLEFLSDNGSAYIAAETRGVA
                     RSLGLKPINTPVCSPQSNGMAESFVNTFKRDYVARMDLSDAATVLAQLPAAFEHFNEV
                     HPHSSLKMRSPREFRRHQAAQARRSTVKDAALRCE"
     gene            1451..1594
                     /locus_tag="PK242_06805"
     CDS             1451..1594
                     /locus_tag="PK242_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93764.1"
                     /translation="MFLRINGWRLHRAPMQIHAEVIEMFETRTFDIAHLDPWLRSFAT
                     LAE"
     gene            complement(1889..1981)
                     /locus_tag="PK242_06810"
     tRNA            complement(1889..1981)
                     /locus_tag="PK242_06810"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1945..1947),aa:Ser,seq:gct)
     gene            complement(2009..3280)
                     /locus_tag="PK242_06815"
     CDS             complement(2009..3280)
                     /locus_tag="PK242_06815"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOZ93765.1"
                     /translation="MALIVQKYGGTSMGSTERIAQVAKRVAKWARAGHRMVVVPSAMS
                     GETNRLLALAKELAPGKPTQDYYREQDMLAATGEQASSALLAIALQAEGQPAVSYAGW
                     QVPIRTSNAYTKARIDSIDDQRVRADLDAGKVVIITGFQGVDADGHITTLGRGGSDTS
                     AVAVAAALKADEAMIFTDVDGVYTTDPRIVPEARRLASLSFEEMLEMASLGSKVLQIR
                     SVEFAGKYKVPLRVLSSFTPWDIDLAEEARSGTLITFEEDEHMEKAVVSGIAFNRDEA
                     KISVLGVPDKPGIAYQILGAVADANIEVDVIIQNISKDGKTDLSFTVNHGDFARAMDL
                     LKDKVAPALGAADVVGDPRICKVSIVGIGMRSHVGVASKMFRALSEEGINIQMISTSE
                     IKTSVVIDEKYMELAVRALHRAFDLDQPSAA"
     gene            complement(3341..4285)
                     /gene="tilS"
                     /locus_tag="PK242_06820"
     CDS             complement(3341..4285)
                     /gene="tilS"
                     /locus_tag="PK242_06820"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOZ93766.1"
                     /translation="MDAFAAEAPALPLAVALSGGADSTALLLACAARWPGQVQAWHVH
                     HGLQPAADDFERHCSALCARLDVPLLRRRVDARPARGQSPEDAARRHRYEAFDALALA
                     SQAQAAIKTVVIAQHADDQVETILLALSRGAGLPGLSAMPARWQRGDVAYRRPLLGVP
                     AAAIRAWLRARGQGWVEDPSNADERYTRNRIRARLLPALQACWPAYRTTFARSAAHAA
                     EAQQILADVAAQDLAHCGVPPRLDALQTLSAPRQANLLRHWLASTHAQTPSSAQLAAL
                     LAQVAAARTRGHRIQLKVGAGRVSRRGAVLHWAPDAER"
     gene            complement(4471..4547)
                     /locus_tag="PK242_06825"
     tRNA            complement(4471..4547)
                     /locus_tag="PK242_06825"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4511..4513),aa:Val,seq:gac)
     gene            complement(4589..6805)
                     /locus_tag="PK242_06830"
     CDS             complement(4589..6805)
                     /locus_tag="PK242_06830"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOZ93767.1"
                     /translation="MKTLSVAPAGTRQSVADVVVATGGGQPEAGELRARARAFAEPLL
                     AGETTASGENVLTHADAVADILAGIGGSEAIQAAAYLSYASAQLNRPEEVIGKAFGDS
                     LARLALETARLEQLQQRSRASRQQLSADTEQASQQTEFVRKMLLAFSRDLRVVLLRLA
                     SRLQTLRYYAAARRPPPPGLLRESHEVFAPLANRLGIWQIKWEMEDLVFRAQEPDTYR
                     HIASLLDEKRVERELALEQRRAAIEAALRAQGIDASVSGRPKHISSIVRKMRGKSLGF
                     EQVFDVRALRILVPTVVDCYTALGWVHQHFTPVPEEFDDYIARPKPNGYRSLHTVVRD
                     PDGRAWEIQIRTHDMHQHAEHGVAAHWAYKEAGTKGYAGVSAAGEYDAKIAVLRQLLA
                     WERELSGAAPQHGLFDDRIYVLTPEAAIVELPAGATPVDFAYTLHTELGHRCRGARVD
                     GAMLPLHTPLKNGQTVEIVAAKEGGPSRDWLNPELRYLASSRARAKVRAWFNAQHTRE
                     TVARGREMVEKLLQREGRTALSLEHLAAELGFKTPEALFETVGKDELSLRAIEAVLRP
                     PEPTPAPDDQVLRRKARAGSAGGRGGVLVVGLDSLLTQPARCCKPAPPDDIAGYVTRG
                     RGVTIHRRDCASFRDLQAREPERVIEVGWSTPATGHLPAYPVDVAVEALDRQGLLRDI
                     SDVFAREKMNVIGVQTQSVKGWAWMTFTVEVSDTQRLAFVLRQVQDVPGVRVAKRR"
     gene            complement(6802..7728)
                     /locus_tag="PK242_06835"
     CDS             complement(6802..7728)
                     /locus_tag="PK242_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ93768.1"
                     /translation="MTEPVLHHVSCPDASGGHRMACWQWGDAAAAHLVVCVHGLSRQG
                     RDFDVLARALLARAAARGQSLRVVCPDVVGRGESDWLKEPMGYQFPTYVGDMLTLLGT
                     LAQQAPIGRFDWVGTSMGGLIGLMLAGAPDLPLPAPLRRLVLNDVGPVIEWQALQRIG
                     QYLGKSGPFESVEAGAACLRALAPGFGPHTDAQWLALSRPMLRARPEGDWRLHYDPAI
                     AVPYAAVTPESSAQGEALLWQLYDRVKADTLLLRGAESDLLSAATASAMQARGPRPRL
                     LEFAGVGHAPTLVADDQVAAVLDFLLPAGEAP"
     gene            complement(7914..8657)
                     /locus_tag="PK242_06840"
     CDS             complement(7914..8657)
                     /locus_tag="PK242_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541752.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOZ93769.1"
                     /translation="MNHRLVRAARALALLLAIAAPAAHAQTHSPATPMCPTPNVPQNV
                     IQLSASGQVEVVQDLLTLTLGTAREGADAAGVQAALRQALDEALRQVGASAPADQMEV
                     RTGAFRVHPRHDRNGRISGWQGRAELVLEGRDFARITQAAARATTMVIDQLAFGLSRT
                     QRARAETEAQTQAIERFKARASDVARAFGFAGYTLREVSVSGDAGDIAPRGFGKASRM
                     EAMAADAPVPVEPGRSLVQVTVSGTVQAH"
     gene            complement(8713..9027)
                     /locus_tag="PK242_06845"
     CDS             complement(8713..9027)
                     /locus_tag="PK242_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93770.1"
                     /translation="MNRWICLLGIWGALALPMAVRAQAVGEASESGPSGAPPAVSEAA
                     LARSPLWQAVESMFRQREVGPVPDRRLDAQQRQQLREQVRRAAARGQRHGVAEVTQLG
                     SR"
     gene            9197..9928
                     /gene="ompR"
                     /locus_tag="PK242_06850"
     CDS             9197..9928
                     /gene="ompR"
                     /locus_tag="PK242_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HOZ93771.1"
                     /translation="MNQSANRTDKIVIVDDDARIRDLLRRYLAQEGFEVIVAEDAKAL
                     ARVMLRDTIDLIVLDLMMPGEDGLSVCRRLRAAGDKTPIIMLTAKGEDVDRIVGLEVG
                     ADDYLGKPFNPRELLARIHAVLRRRPPLEAPGAPSAENESVAFGPFQFDLGARTLHKN
                     GEEVPLTTGEFAMLKALVRHPRQPLSREKLAQLARGREFEPFDRSLDVQVSRLRKLIE
                     QDAASPRYIQTVWGVGYVFVPDGTG"
     gene            10042..11430
                     /locus_tag="PK242_06855"
     CDS             10042..11430
                     /locus_tag="PK242_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ93772.1"
                     /translation="MEPEVEARAPLAFSLFWRTFFLLALLLLGTTVGWYQMFRALEYE
                     PRVIGNARQIASLVNLSRAALIHSDAIARVSLIKTLAEQEKVRIAPREPGDRFELFNQ
                     TVLERRMSGEIVARLGLGTVVASRVNGEEGLWVGFSIEGDSYWLLMDRSRIGTLLSGS
                     AWLLWLATLGAASLLGAALLAGYINRPLRQLSNAAARVREGDYLEGRLDETTRAREIR
                     AVNIGFNRMAEELSRIEQERAEMLAGISHDLRTPLARLRLETELSVPDAEAREHMVAD
                     IAQVDTIIDKFLDYARPDHVSLQALPLAALAQAAAQPFASRDDMQVQIDVPPELRVMG
                     DEVELSRVLANLLENARRYGKSADSGIARVRIAATARDQWVTLRVRDHGPGVPEEQLA
                     VLTRPFFRGDTARTEATGAGLGLSIVAKMVRHMGGELNLTNSPSGGLMAVIRLPQASD
                     RRADHGSLRKRR"
BASE COUNT         1812 a         4054 c         4133 g         1759 t
ORIGIN      
        1 ctggaaagct tggcaatcaa ccatccattc gtggatggca acaaacgcat cgcctgagat
       61 agccccctga aaccccggac ccgtcctatc gcttatcttt gaagatagga gtaggactgt
      121 gagtacgacc aggcatacgg acactgttga agtcatcttg aaagaccagc gccgcaggcg
      181 ctggtcccct gcggagaaag ccgcgctggt gcggcgcacc tatgagccgg gcatgagcgt
      241 gtcgctggtg gcgcgtcagg aaggagtggc tgccagcttg ctgttccagt ggagaaagct
      301 cgatcgccaa ggcgccctga cggccgtcag tgccggggaa gcggtggttc cggcctcgga
      361 actggctgct gcccgagccg agatcgccaa gctccagcgg gtgctgggca agaagaccct
      421 ggagaacgaa atcctcaagg aagccgtgga gtacgccgcc gaaaaaaagt ggattgcgcg
      481 ctcgcccttg ttgcccgggg acgaccaatg aaaacggtct gccgggcgct gggcgtggcg
      541 cgctcgcatg tgcatgagtt gcatcaccgc agtcctgact ggcgtgatgg tcgcaagggg
      601 cgcacgccag cgggcgatgc gcagttgctg gccgagatcc gggagcggat cgcagagcta
      661 cccagctacg ggtatcgacg cgcttgtgcc ctggtcaacc gccagcgcag cacacaaggc
      721 gacgcacggg tcaatcccaa gcgcgcctac cgcgtgatgg ccctggccgg gctgctgctg
      781 cccaaggcgc cccgacggcg ccagtccagc cgaacacacg agggcaccgt ggccgtgggg
      841 cgcagcgatc tgcgctggtg ctccgatggg ctggagatca agtgcgactc gggacagacg
      901 gtgacgacca cgttcgccaa agattgctgc gaccgcgaag tgatcgcctg gcgagcatgg
      961 gagggcaaag ggctgcccgg tgagccggtg cgcgagatgt tgatcgaggc ggtggagaaa
     1021 cgctttggat cggtggaggc ggtgccccct gatcactggc tggaattcct cagcgacaac
     1081 ggcagcgcct acatcgcggc cgagacccga ggggtggccc gctcactggg cctcaagccg
     1141 atcaacacgc cggtgtgcag tccgcagagc aacggcatgg ccgagagctt cgtcaacacg
     1201 ttcaagcgag actacgtggc ccgcatggac ctgagcgacg cggccaccgt gctggctcag
     1261 ttgccagcgg ccttcgagca cttcaacgag gtgcatccgc actcgtcgtt gaaaatgcgt
     1321 tcacccagag agttccggcg gcatcaggcc gcccaggcac gccgctcaac cgtcaaggat
     1381 gcggcgttac gttgcgaata gggcgggtcc gggaataccg gggcaagatc acgccttcgc
     1441 cgctgccgac gtgtttctgc gcatcaatgg ctggcgcttg catcgtgctc cgatgcagat
     1501 tcatgccgag gtgatcgaga tgttcgagac cagaacattc gacattgccc acctcgatcc
     1561 ctggctgcgg tctttcgcca cccttgcaga gtgatgccac gaaggaaaaa gcacccaata
     1621 tccagacaca ggctgcgatg gcaatccccc cacaggttgg ctgacgccag aaaccgggtt
     1681 ctctcgtacc cttacccgag gaggctccat gaagaaatcc cgtttcaccg acagccagat
     1741 caacgcctca tgcacgccaa agccccgtaa cctcaggttc cggggctttt tttgtcgtgg
     1801 ctgcgtcgcc agcccgcgcg caggcaggca tgacagacag atgcgggcat gaaaaaagcg
     1861 gcccgtgggc cgctcgtgca ggctcttctg gcggagacgg agggattcga accctcgatg
     1921 aggctctaca ccccatactc ccttagcagg ggagcacctt cggccactcg gtcacgtctc
     1981 cagaaggcaa gcgcgcatta tgccaccatc aggcggcgct gggctgatcg aggtcgaacg
     2041 cgcggtgcag ggcgcgcacc gccagttcca tgtatttctc gtcgatgaca accgaggtct
     2101 tgatctcgct ggtggagatc atctgaatgt tgatgccttc ctcgctcagc gcgcggaaca
     2161 tcttgctggc cacgccgacg tggctgcgca tgccgatgcc gacgatgctg accttgcaga
     2221 tgcgcgggtc acccaccacg tcggcggcgc ccagcgccgg ggccaccttg tccttcagca
     2281 ggtccatcgc gcgggcgaag tcgccgtggt tgacggtgaa cgacaggtcg gtcttgccgt
     2341 ccttgctgat gttctgaatg atgacgtcga cctcgatgtt ggcgtcggcc accgcaccca
     2401 ggatctggta ggcgatgccc ggcttgtcgg gcacgcccaa caccgaaatc ttggcttcgt
     2461 cacggttgaa ggcaatgccg gaaacgacgg ctttttccat gtgttcgtct tcctcgaaag
     2521 tgatcagggt gccggatcgg gcctcttcgg cgaggtcgat gtcccagggc gtgaagctgg
     2581 acagcacgcg cagcggcact ttgtacttgc cggcgaattc gaccgagcgg atttgcagca
     2641 ccttgctgcc caggctggcc atttcgagca tttcctcgaa gctcaggctg gccagacggc
     2701 gcgcctcggg cacgatgcgc ggatcggtgg tgtagacgcc atcgacgtcg gtgaagatca
     2761 tggcctcgtc ggccttgagc gcggccgcca cggcgacggc ggaggtgtcg ctgccgccac
     2821 gacccagcgt ggtgatgtgg ccgtcggcgt ccacgccctg aaagccggtg atgatgacca
     2881 ccttgccggc gtccaggtcg gcgcgcacgc gctggtcgtc gatgctgtcg atgcgtgcct
     2941 tggtgtaggc gttgctggtg cggatcggca cctgccagcc ggcgtagctg acggccggct
     3001 ggccctcggc ctgcagcgcg atggccagca acgccgacga ggcctgctcg ccggtggcgg
     3061 ccagcatgtc ttgctcgcgg taatagtcct gcgtcggctt gccgggggcc agttccttgg
     3121 ccagcgccag caggcggttg gtttcaccgc tcatggcgct gggcacgacc accatccggt
     3181 ggccggcgcg cgcccatttg gccacgcgct tggcgacctg cgcaatgcgc tcggtggagc
     3241 ccatcgaggt tccaccgtac ttctgaacga tcaatgccat ggaatgcctg gaaaagacga
     3301 aatcggtgcg cgccagggct ggcgcggcaa acccggcatt ttagcgttcg gcgtccggcg
     3361 cccagtgcag caccgcgccg cgacgcgaca cccggccggc gcccaccttg agctggatgc
     3421 ggtgaccgcg cgtgcgcgcc gccgccacct gcgccagcag cgccgccagt tgcgcgctgc
     3481 tgggcgtctg ggcgtgcgtg ctggccagcc agtggcgcag caggttggcc tggcgcggcg
     3541 cggacagcgt ctgcagtgcg tccagacgcg gcggcacgcc gcagtgcgcc aggtcctgtg
     3601 ccgccacgtc ggcaaggatc tgctgcgcct cggcggcgtg ggcggcgctg cgggcgaagg
     3661 tggtgcgata ggccggccag caggcctgca gcgccggcag caggcgcgcg cggatgcggt
     3721 tgcgggtgta gcgctcgtcg gcgttgctcg ggtcctcgac ccagccctgg ccgcgcgcgc
     3781 gcagccaggc gcgaatggcc gccgccggca cgcccagcag cgggcgccgg tacgccacgt
     3841 cgccgcgctg ccagcgcgcc ggcatggccg acaggccggg cagcccggcg ccgcgcgaca
     3901 gcgccagcag gatggtttca acctggtcgt ccgcatgctg cgcgatgact actgttttga
     3961 tagctgcttg cgcttgactg gcaagcgcca gagcatcaaa tgcctcgtaa cgatggcggc
     4021 gcgccgcgtc ctccgggctc tggccgcggg ccggccgcgc atcgacacgg cgccgcagca
     4081 ggggcacgtc caggcgcgca cacagcgcgc tgcagtggcg ctcgaagtcg tcggccgccg
     4141 gctgcaggcc gtggtgcaca tgccaggcct gcacctgccc cggccagcgc gccgcgcagg
     4201 ccagcagcag cgcggtcgaa tcggcgccgc cgctcagggc caccgccagc ggcagcgcgg
     4261 gcgcctcggc cgcgaaggcg tccatggcgg cgtcgacgcg ggccggatcg gcgtcgctgg
     4321 cgcgaagtga cgaggtggga cgggacatgc cgggcgggtg ccgcaccaga gggtgggcct
     4381 ggccgtgatg gtatcggccc gcacccgccg ctgcggcggc gcccaaaaca aaaggcaccc
     4441 ggacttgcgt tcgggtgcct tgcagggttg tggtaggcgc gattggattc gaaccaacga
     4501 cccccaccat gtcaaggtgg tgctctaacc aactgagcta cgcgcctgaa aatcgattcg
     4561 aagcccgcaa gtatagcgaa ttccggcgtc agcgccgttt ggccacgcgc acgccgggca
     4621 cgtcctgcac ctggcgcagc acgaaggcca ggcgctgggt gtcgctgacc tcgaccgtga
     4681 aggtcatcca ggcccagccc ttgacggact gcgtctgcac gccgatgacg ttcatcttct
     4741 cgcgcgcgaa gacgtccgag atgtcgcgca gcaggccctg gcggtccagc gcctccaccg
     4801 ccacgtcgac cggataggcc ggcaggtggc cggtggccgg cgtgctccag ccgacttcga
     4861 tcacgcgctc gggctcgcgc gcctgcaggt cgcgaaagct ggcgcagtcg cgccggtgga
     4921 tggtgacgcc ccgcccgcgc gtgacgtagc cggcgatgtc gtcgggcggc gccggcttgc
     4981 agcagcgcgc cggctgcgtc agcagcgaat ccagccccac caccagcacg ccaccgcgac
     5041 cgcctgcgct gccggcgcgc gccttgcgcc gcagcacctg gtcatcgggc gccggcgtcg
     5101 gctcgggcgg gcgcagcacg gcttcgatgg cgcgcagcga cagctcgtcc ttgcccacgg
     5161 tctcgaacag cgcctcgggc gtcttgaaac ccagttcggc cgccaggtgc tccagcgaca
     5221 gcgccgtgcg gccttcgcgc tgcagcagct tttcgaccat ctcgcggccg cgcgccacgg
     5281 tttcgcgcgt gtgctgggcg ttgaaccagg cgcgcacctt ggccctggcg cgcgaggacg
     5341 ccaggtagcg cagctcgggg ttgagccagt cgcgcgaggg cccgccttcc ttggcggcga
     5401 cgatctccac cgtctggccg ttcttcagcg gcgtgtgcag cggcagcatg gcgccgtcga
     5461 cgcgcgcgcc gcggcagcgg tggcccagct cggtgtgcag cgtgtaggca aagtccaccg
     5521 gcgtggcgcc ggccggcagc tcgacgatgg ccgcctcggg cgtcagcacg tagatgcggt
     5581 cgtcgaacag gccgtgctgc ggcgcggcgc cggacagctc gcgctcccag gccagcagct
     5641 ggcgcagcac ggcgatcttg gcgtcgtatt cgccggccgc gctgacgccg gcgtagccct
     5701 tggtgccggc ctccttgtag gcccagtggg ccgccacgcc gtgttcggcg tgctggtgca
     5761 tgtcgtgggt gcggatctgg atttcccagg cgcggccgtc ggggtcgcgc accacggtgt
     5821 gcagcgagcg gtagccgttg ggcttgggac gcgcgatgta gtcgtcgaac tcctccggca
     5881 ccggcgtgaa gtgctgatgc acccagccca gcgcggtgta gcagtcgacc acggtgggca
     5941 ccaggatgcg cagcgcgcgc acgtcgaaca cctgttcgaa gccgagcgac ttgccgcgca
     6001 tcttgcgcac gatgctgctg atgtgcttgg gtcggccgct gacgctggcg tcgatgccct
     6061 gggcgcgcag cgccgcttcg atggcggcgc ggcgctgctc cagcgccagt tcgcgctcga
     6121 cgcgcttttc gtccagcagg ctggcgatgt ggcggtaggt gtcgggttcc tgggcgcgga
     6181 acaccaggtc ttccatttcc cacttgatct gccagatgcc caggcggttg gccagcgggg
     6241 cgaacacctc gtgcgattca cgcagcagac cgggcggcgg cgggcggcgc gcggcggcgt
     6301 agtagcgcag cgtctgcagg cgcgaagcca ggcgcagcag caccacgcgc agatcgcgcg
     6361 aaaaggccag cagcatcttg cgcacgaact cggtctgctg gctggcctgc tcggtgtcgg
     6421 ccgacagctg ctggcggctg gcgcgcgagc gctgctgcaa ctgctccagc cgcgcggttt
     6481 cgagcgccag gcgcgccagg ctgtcgccga aggccttgcc gatcacctcc tccggccggt
     6541 tcagttgcgc gctggcgtag ctcaggtagg ccgccgcctg gatcgcctcg gagccgccaa
     6601 tgcccgccag gatgtcggcc accgcgtcgg cgtgggtcag cacgttctcg cccgaggccg
     6661 tggtctcgcc cgccagcagc ggctcggcga aggcgcgcgc gcgtgcgcgc agctcacccg
     6721 cctcgggctg gccgccgccg gtggctacca ccacgtcggc caccgactgg cgcgtgccgg
     6781 caggcgccac cgacagtgtc ttcatggcgc ttcgcccgcc ggcagcagga agtcgagcac
     6841 ggccgccacc tggtcgtcgg ccaccagcgt gggcgcgtga cccacgccgg caaattcgag
     6901 caggcgcgga cgcgggccgc gcgcctgcat ggcgctggcc gtggcggccg acagcaggtc
     6961 ggattcggcg ccgcgcagca gcagggtgtc ggccttgacc cggtcgtaca gctgccacag
     7021 cagggcctcg ccctgggccg acgactcggg tgtgacggcg gcgtagggca cggcgatggc
     7081 cgggtcgtag tgcaggcgcc agtcgccttc gggccgggcg cgcagcatgg ggcgcgacag
     7141 cgccagccat tgggcgtcgg tgtgcgggcc gaagccgggc gccagcgcgc gcaggcatgc
     7201 cgccccggcc tcgaccgact cgaacggacc gctcttgccc aggtactggc cgatgcgctg
     7261 cagcgcctgc cattcgatca ccggaccaac gtcgttgagc accagccggc gcagcggcgc
     7321 cggcagcggc agatcgggcg cgccggccag catcaggccg atcagtccgc ccatgctggt
     7381 gccgacccag tcgaagcgcc cgatcggcgc ctgctgcgcc agcgtcccca gcagggtcag
     7441 catgtcgccc acgtaggtgg ggaactggta gcccatgggc tccttcagcc agtcgctctc
     7501 gccgcgcccg accacgtccg ggcagaccac gcgcagggac tggccgcgcg ccgccgcgcg
     7561 cgccagcagg gcgcgcgcca gcacgtcgaa atcgcgcccc tggcgcgaca ggccatgcac
     7621 gcacaccacc aggtgcgcgg ccgccgcatc gccccactgc cagcaggcca tgcggtggcc
     7681 accgctggcg tcgggacagg aaacatggtg caggacgggt tcggtcatgg cggcgggtcg
     7741 ctcggctggc gccccggcga cgacggccgg ggcagggtcg caatcatggt aaatcaactg
     7801 gcccccaggg ggcgcaccgg ggtggcccgc agaagacctg gtgaactatc aaaacgatag
     7861 cttctggcgc tgtatgggta agcgctggcg gccaatttga ctgggaaccg acatcaatgt
     7921 gcctgcaccg tgcccgagac cgtcacctgc accaggctgc gccccggctc caccggcacc
     7981 ggagcgtcgg cggccatggc ctccatcctg ctggccttgc cgaacccgcg tggcgcgatg
     8041 tcgcccgcgt ccccgctgac gctgacctcg cgcagcgtgt agccggcaaa gccgaaggcg
     8101 cgcgccacgt cggacgcccg cgccttgaag cgttcgatcg cctgcgtctg cgcctcggtt
     8161 tcggcacggg cgcgctgggt gcggctcagg ccaaaggcaa gctggtcgat caccatcgtg
     8221 gtggcgcgcg cggccgcctg cgtgatgcgg gcgaagtcgc gcccttccag caccagctcg
     8281 gcacggccct gccagccgct gatgcggccg ttgcggtcat ggcgcggatg cacacggaag
     8341 gcgccggtgc gcacctccat ctgatcggcc ggcgcgctgg cgccgacctg ccgcagcgcc
     8401 tcgtccagcg cctgcctcag cgcggcctgc acgccggcgg cgtcggcgcc ttcgcgcgcg
     8461 gtgccgagcg tcagggtgag cagatcctgc accacctcga cctggccact ggcgctgagc
     8521 tgaatcacgt tctgtggcac gttcggggtc ggacacatgg gtgtggcggg gctgtgggtc
     8581 tgggcatggg ctgcgggcgc ggcgatggcc agcagcaggg ccagcgcgcg ggcggcgcgt
     8641 acaagtcgat ggttcatggg ataagcgcag tcggcgcgcc cggcggcgcg gggcgccatg
     8701 atggcacagg gttcagcggg acccgagttg ggtgacttcg gccacgccgt ggcgctggcc
     8761 gcgggcagcg gcgcgacgca cctgctcgcg cagctgctgg cgctgctggg cgtccagacg
     8821 acgatcgggc accgggccga cctcgcgctg gcggaacatc gactccaccg cctgccacag
     8881 cgggctgcgc gccagtgccg cctcggacac cgcgggcggt gcgccgctgg ggccggattc
     8941 cgacgcttcg cccacggcct gcgcgcgcac ggccatcggc agcgccagcg cgccccagat
     9001 tccaagcaaa cagatccagc ggttcatgtg catatcaacc gctgaccatc ccacaccgat
     9061 gacgtgagcg tccggcagat agctgacccg tggggcaaca ggtgtaacac tgtgaaaagc
     9121 agcccgtgaa aaccctgaat tcctcacgct ttcggggtca aaatgggcct gttacaaatt
     9181 cgctgaaagg tgctggatga accagtctgc caaccgaacc gacaagatcg tcatcgtcga
     9241 cgacgatgcc cgcatccgcg atctgctgcg gcgctacctg gcgcaggaag gcttcgaagt
     9301 catcgtggcc gaggacgcca aggcgctggc gcgcgtgatg ctgcgcgaca ccatcgacct
     9361 gatcgtgctc gacctgatga tgccgggcga agacggcctg tcggtgtgcc ggcgcctgcg
     9421 cgcggcgggc gacaagacgc ccatcatcat gctcaccgcc aagggcgagg acgtggaccg
     9481 catcgtcggc ctcgaagtcg gcgccgacga ctacctgggc aagccgttca acccgcgcga
     9541 gctgctggcg cgcatccacg ccgtgctgcg ccgccgtccg ccgctggaag cgccgggcgc
     9601 accctcggcc gagaacgagt cggtcgcctt cgggccgttc cagttcgacc tgggcgcgcg
     9661 caccctgcac aagaacggcg aggaagtgcc gctgaccacc ggcgaattcg ccatgctcaa
     9721 ggcgctggtg cgccacccgc gccagccgct gtcgcgcgag aagctggcgc agctggcacg
     9781 cgggcgcgaa ttcgagccct tcgaccgcag cctggacgtg caggtgtcgc gcctgcgcaa
     9841 gctgatcgag caggacgccg ccagcccgcg ctacatccag accgtgtggg gcgtgggcta
     9901 cgtgttcgtg cccgacggca ccggctgacc accgtgcccg agcgcgcctt cggcgtgtcc
     9961 gcgggttcga cgctgccgcc gacggacatc gagccgccga ccacgcgccc ggacctgctg
    10021 cccgactcca gcaacgcgcc gctggagccg gaggtcgagg cgcgcgcgcc actggccttc
    10081 agcctgttct ggcgcacctt ctttctgctg gcgctgctgc tgctgggcac cacggtcggc
    10141 tggtaccaga tgttccgagc gctcgagtac gagccgcgcg tgatcggcaa cgcgcgccag
    10201 atcgcctcgc tggtcaacct ctcgcgcgcg gcgctgatcc actccgacgc catcgcccgc
    10261 gtgtcgctca tcaagacgct ggccgagcag gaaaaggtgc gcatcgcgcc gcgcgagccg
    10321 ggcgaccgct tcgagctgtt caaccagacc gtgctggagc gccgcatgag cggcgagatc
    10381 gtggcccgcc tgggcctggg cacggtggtg gccagccggg tcaacgggga agaggggctg
    10441 tgggtcggct tttcgatcga gggcgactcg tactggctgc tgatggaccg ctcgcgcatc
    10501 ggcaccctgc tcagcggcag cgcctggctg ctgtggctgg ccacgctggg cgccgcctcg
    10561 ctgctgggcg cggcgctgct ggccggctac atcaaccggc cgctgcgcca gctgtcgaat
    10621 gccgccgcgc gcgtgcgcga gggcgactac ctggaaggcc ggctcgacga aaccactcgc
    10681 gcgcgcgaga tccgcgccgt caacatcggc ttcaaccgca tggccgagga gctgtcgcgc
    10741 atcgagcagg agcgcgccga gatgctggcc ggcatctcgc acgacctgcg cacgccgctg
    10801 gcgcgcctgc ggctggaaac cgagctgagc gtgcccgacg ccgaggcgcg cgagcacatg
    10861 gtggccgaca tcgcccaggt cgacaccatc atcgacaagt tcctcgacta cgcccggccc
    10921 gaccacgtga gcctgcaggc gctgccgctg gcggcgctgg cacaggccgc ggcccagccc
    10981 tttgccagcc gcgacgacat gcaggtgcag atcgacgtgc cgcccgagct gcgcgtgatg
    11041 ggcgacgagg tggagctgtc gcgcgtgctg gccaacctgc tggaaaacgc gcgccgctac
    11101 ggcaagagcg ccgacagcgg catcgcccgc gtgcgcatcg ccgccaccgc gcgcgaccag
    11161 tgggtgacgc tgcgcgtacg cgaccacggc cccggcgtgc ccgaggagca gctggccgtg
    11221 ctcacgcgcc cgttcttccg tggcgacacc gcccgcaccg aagccaccgg cgccggcctg
    11281 ggcctgtcca tcgtcgccaa gatggtgcgc cacatgggcg gtgaactgaa cctgaccaac
    11341 agccccagtg gcggcctgat ggcggtgatc cggctgccgc aggcgtccga caggcgcgcc
    11401 gaccatggct ccctgcgcaa gcgccgctga ttcagcctgg aaacggcagt tggacgcgcc
    11461 cgcccgtgcc gcgatcggcg tgccaggcaa gacgcgacgc cgccgcgggc tcgtgcccgc
    11521 aagcaggagc aacgccgcat ggcgcggcga gcgcggtgcg ggtgggtgcg cagcgcgctc
    11581 acccaggagg tgaaggccat cgcagccgcc agaccccgca tcgatgcggt ctgcccgaag
    11641 ttgaccagcg gtcaactgcc gtttctagga ggctgtcggg cttggagcgc cgaaagcgtt
    11701 ctcgaagagc ggcgtagcca aaatcggccc cagcgagacc agtttttgcc ggatttgc
//
LOCUS       DAOOOG010000070        11731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6101_length_11731_cov_32.411014, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000070 DAOOOG010000000
VERSION     DAOOOG010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6101_length_11731_cov_32.411014"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /gene="dacB"
                     /locus_tag="PK242_06860"
     CDS             <1..1431
                     /gene="dacB"
                     /locus_tag="PK242_06860"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOZ93773.1"
                     /translation="PDIAQALAKAGVPTSAVAMVVAPLPPPPGSVTRQPEPVNPSGEA
                     NPAPAAASLALPPPRLAWQPERPMNPASVMKLVTTYAGLDLLGAGHFWKTRVYTLGTL
                     REGTLLGNLLIQGGGDPKLVVERLQELLRAIQDQGVRRIEGDILLDNSVFRLPPHNAA
                     AFDDDPLRPYNVGPDGLLLNFKALVLRFIPEPAQRRVRIESQPPMAGVGVPAELPVTS
                     GACGDWRTRLALDFSDPEQLRLGGRYPLACGEQEWAIAYPQPERYAPRVIEAMWRAAG
                     GALSGQVRWAEQRPVGARVLVTGLSLPLASVIADINKYSNNVMAQQLFLTLSAGDGRA
                     SFAESRNLLRRWWRERFGLRTTPLVDNGSGLSRDERATAAALAALLQQAASHPQGAVF
                     EQSLSLAGVDGTTRRLAQRRPGSAAIGNARLKTGSLRDVAAIAGYAYGQSGQNYAVVG
                     LINHPNAGAARAALDRLVEWAVQDAP"
     gene            1702..2160
                     /locus_tag="PK242_06865"
     CDS             1702..2160
                     /locus_tag="PK242_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93774.1"
                     /translation="MKPLALSLLLTSAALLPLHVHADTLAPAPETPAAAFQRLFAHTP
                     GPVRPPAGAAPDALTRAFNEALWSTPDQVPTIHQAAFDRAGLPPTAQAPESPAVAFQR
                     MLGHTPGPVQPPVGGAPDVLTRVFNDALWSTPATAAAYASARQHTLERAQ"
     gene            2157..2762
                     /locus_tag="PK242_06870"
     CDS             2157..2762
                     /locus_tag="PK242_06870"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HOZ93775.1"
                     /translation="MNHRLLLAAHLVGAAALGAAIVALWWNDRRIARAGDCGTLAGAG
                     RRAQAIVQRLLLPGVLLLAASGVWLLARYYGWSFVRLPWLAAMAALFVFQSVWANTVT
                     RHHGQRLGRLLAGRCATDRLTPELERARRAPLARFGQQLEPLLFALIVAFGLARPMDW
                     VAIAAGVAAAGLLAAALTAYTLRPPASSSFSSASVEAAGRA"
     gene            complement(2693..5902)
                     /locus_tag="PK242_06875"
     CDS             complement(2693..5902)
                     /locus_tag="PK242_06875"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOZ93776.1"
                     /translation="MGTDDARRPLSLAKLTAPRPRHAWPRRRLFERLDAARRGGTVIW
                     IAAPAGSGKTTLVASYLQARRLRAVWYRIDAADADPASFFHDLAQAAGSRRRADALPP
                     FTAEYLAGLEVFARNWFRRFFARLQGTPLLVLDDHHLIDADSPVHLALREGLAEVPPG
                     MAVIVTSRAEPPALLTRLRLQEGFTLLDGEALRLSADECVGVARQRLAGAAPADEVLQ
                     QLHQRTQGWVAGLVLMLERGAAATLDEATPAAEPLVFDYFAGELLAQADARVQRFLLL
                     TALLPQVSARAAAALTAQQDAAALLAELERRNFFVARHAEPGRGEAWQYHPLFREFLL
                     ARGRRELTDDELAGLRSQAAALLAAEGDAPAAVALLREAGDWAQLVPLVLEQAPALLR
                     SGRFQTLGEWILAIPQAQRAVSPWLDYFLGLCRLPYDPLQARTLLSSAFTGFRAAGHA
                     AGEYLSWAGVVDTFLYAWSDFGPADPWIEEFDALRQRHPQFPSREAELRAVAAIFALL
                     MYRRPQHPELPQWTHRLDALVAQDRGEPAWRMTAAVHLVLYYEWWVGKLARANELVRR
                     LEPFASARDVGPFVRLAWEGILAISHWMNADNEAALAAVRRGLALADETGAHVWDFML
                     VAQGAVAAGTSGDAGQARDFLERLGERAPGYRPLDSVQYHFFSFQQAMRRDDRAAMLA
                     HGRAGLASALAAGVVWAEVYTRPALAQALFLAGDEAGARREIERATQVAAAVGCSNAF
                     YYIHELEAQFAEARGAHDAQRAALARMFQVMREQGFCNSAWWRDEPMARWCRIALEQG
                     IDVEFVRALIRRRGLRPDGPAARLEHWPWPLRLRTLGRFTIELDGQPLAFSGKVQKRP
                     LALLKALVAFGAQGVPEEQLAEALWPDAEGDDAHHAFTTTLQRLRRLLGQHEALLLQE
                     GRLSLNPQLCWVDAQAFESIDPDARDPAVGPDRERALRLYRGAFLAQEDAAWAIAPRE
                     RLRARFVRLGGGDVGQHLAAADWEGAVACLERLLHADPTVEPFYQQLMRAQHRLGRSA
                     QVLATYRRCREVLGATLQARPAASTEALLKELDAGGRNV"
     gene            complement(6395..7477)
                     /locus_tag="PK242_06880"
     CDS             complement(6395..7477)
                     /locus_tag="PK242_06880"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HOZ93777.1"
                     /translation="MTPPRTHRIAVIAGDGIGQEVMPEALRAIDAVQRRFGFALQQTP
                     IAWASCDYYARTGQMMPDDWKAQLQGMDALLFGAVGWPATVPDHVSLWGSLLKFRREF
                     DQYINLRPVRLFDGVPCPLAGKKPGDIDFLVVRENTEGEYTNLGGVMYAGTEREIVVQ
                     ETVMSAFGADRVLKFAFEQAAARPRHQLDVATKSNGIAISMPWWDGRADAMAARYPQV
                     AVQKHHIDILTARFVLQPERFDVVVASNLFGDILSDLGPACTGTIGLAPSANLNPERR
                     FPSLFEPVHGSAPDIFGRGIANPVAMIWSAALMLQFLGEQAAHDAILRAIEQVLAAGP
                     RTPDLGGTASTVQMGQAIAARIEAGA"
     gene            complement(7571..8479)
                     /locus_tag="PK242_06885"
     CDS             complement(7571..8479)
                     /locus_tag="PK242_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOZ93778.1"
                     /translation="MNASWFRRAALALGCAGTLALLTACGSGSVVSDLAPKRFLTVGD
                     SFMDVGQSGKRYTVNDGSNNWVQELAAHYGVTVSAASAGGSGHAQGHARIAAADPIGG
                     APSVTTQIDTLLASTTLSDTDDVVLINGGMHDIVAAVEAGGLSDATTQAVKDAGKALA
                     TQVRRVVSAGGKHVVVTGVPLLGDSPWARARNQRDGINALSVAFNDALLSSIVDLGAH
                     VLYFDAAFFFNLMYGKPQNFDLANGLDPVCTTPDASTCTAATVASADYNRWLFADGLH
                     FSPGAQRKFASNDYAESAYRRFKERW"
     gene            complement(8642..9634)
                     /locus_tag="PK242_06890"
     CDS             complement(8642..9634)
                     /locus_tag="PK242_06890"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOZ93779.1"
                     /translation="MILDGARPEAIAQAVDALAAGRLVGLPTETVYGLAADAGNAAAV
                     RAIFATKGRPADHPLIVHVAGAAAVPAFASAVPDFAQRLIDACWPGPLTLILPRRPGV
                     ADAAAGGHPSIGLRCPAHPVARALLAACAARGIAGLAAPSANRFGRVSPTTAAHVAAE
                     FGDELLVLDGGACNVGIESTIIDATRGAPVLLRPGQLARADIERLAGRQLLSKEELMA
                     PDPQAPGTLSAHYAPTARLRLMDARELRAALQVLGAEARHIAVWARAPLTSASRQLLL
                     RRMPERADAAARELFAVLREFDAAGARLIWVETPPDDPAWDGVRDRLQRAAASA"
     gene            complement(9631..10833)
                     /locus_tag="PK242_06895"
     CDS             complement(9631..10833)
                     /locus_tag="PK242_06895"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HOZ93780.1"
                     /translation="MSIPPPLLPGTLIDGRPATLGVLGGGQLGRMFVHAAQAMGYETA
                     VLDPDPQSPAGRVSQHFIQADYRDGAGLAELARRAHAISTEFENVPADSLAQLAGTRP
                     VAPAAAAVAIAQDRSAEKAHFARCAGAGGVAPAAHATITSEAELAAVPATLLPGILKT
                     ARLGYDGKGQARVATRAELAAAWQSMGCVPCVLEQRLPLALECSVIVARGRDGQAVSL
                     PVQRNLHRDGILAVTEVHEGNLPPALASQAQAATIKIANQLDYVGVLCVEYFVLEGGA
                     LVVNEMAPRPHNSGHYSLDACDVSQFELQVRTLAGLPLRAPRQHSPAIMLNLLGDLWF
                     DAAGRPRTPPWDALLQLPGAHLHLYGKTEARRGRKMGHLTFTGASTEAVRASARQAAA
                     LLGMEAIG"
     gene            complement(10994..11485)
                     /gene="purE"
                     /locus_tag="PK242_06900"
     CDS             complement(10994..11485)
                     /gene="purE"
                     /locus_tag="PK242_06900"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOZ93781.1"
                     /translation="MTTPLVGVVMGSSSDWDTMQHAVQILEQFGVPHEARVVSAHRMP
                     DDMFAYAEAAPQRGLRAIIAGAGGAAHLPGMLAAKTVVPVLGVPVASRHLQGVDSLHS
                     IVQMPKGVPVATFAIGAAGAANAALFAVALLATTDAALTQRLQTFRAEQTAAARAMTL
                     PPA"
     gene            11539..>11731
                     /locus_tag="PK242_06905"
     CDS             11539..>11731
                     /locus_tag="PK242_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOZ93782.1"
                     /translation="MIDVTVQNFEAEVIAASMHTPVLVDFWAPWCGPCKTLGPILEQL
                     EVEYAGRFKLVKIDSDQQQE"
BASE COUNT         1608 a         4215 c         4347 g         1561 t
ORIGIN      
        1 cccgacatcg cccaggcgct ggccaaggcc ggcgtgccga ccagcgccgt ggccatggtg
       61 gtggcgccgc tgccgccgcc accgggcagc gtgacgcgcc agccggagcc ggtcaacccc
      121 agcggcgaag ccaacccggc accggccgct gccagcctgg cgctgccacc gccgcgcctg
      181 gcctggcagc ccgagcggcc gatgaaccct gcgtcggtga tgaagttggt gaccacttac
      241 gccgggctgg acctgctggg cgcgggccac ttctggaaga cccgggtcta caccctgggc
      301 acgctgcgcg agggcacgct gctcggcaac ctgctgatcc agggcggcgg cgaccccaag
      361 ctggtggtcg agcgcctgca ggagctgctg cgggccatcc aggaccaggg cgtgcgccgc
      421 atcgagggcg acatcctgct ggacaacagc gtgttccgcc tgccgccgca caacgccgcc
      481 gccttcgacg acgaccccct gcgcccctac aacgtggggc cggacgggct gctgctgaac
      541 ttcaaggcgc tggtgctgcg cttcatcccc gagccggcgc agcgccgcgt gcgcatcgaa
      601 agccagccgc ccatggccgg cgtcggcgta ccggccgagc tgccggtcac cagcggcgcc
      661 tgcggcgact ggcgcacgcg gctggcgctc gacttcagcg accccgagca gctgcggctg
      721 ggcgggcgct atccgctggc ctgcggcgag caggaatggg ccatcgccta cccgcagccc
      781 gagcgttacg cgccgcgcgt gatcgaggcc atgtggcgcg ccgccggcgg cgccctgagc
      841 gggcaggtgc gctgggccga gcagcgcccg gtcggcgcac gcgtgctggt gaccggcctg
      901 tcgctgccgc tggccagcgt gattgccgac atcaacaagt actccaacaa cgtgatggcg
      961 cagcagctgt tcctgacact gtcggcgggc gacggtcgcg ccagcttcgc cgaatcgcgc
     1021 aacctgctgc ggcgctggtg gcgcgagcgc ttcgggctgc gcaccacgcc gctggtggac
     1081 aacggctccg gactgtcgcg cgacgaacgc gccaccgcgg ccgcgctggc ggcgctgctg
     1141 cagcaggcgg ccagccatcc gcagggggcg gtgttcgagc agtcgctgtc gctggcgggg
     1201 gtggacggca ccacgcgccg gctggcccag cgccgcccgg ggtcggcggc gatcggcaac
     1261 gcgcgcctga aaaccggcag cctgcgcgac gtggccgcca tcgccggcta cgcctatggc
     1321 cagtcgggcc agaactacgc cgtggtcggc ctcatcaacc accccaacgc cggcgccgcc
     1381 cgcgcagcgc tggaccggct ggtggagtgg gcggtgcagg acgcgccctg agcgcgcctg
     1441 gcgtcagacc ttcagagcga tttcggcctc cagggcccgt cgggcaagcg ccgcaagcta
     1501 tccattacat agcatttggg gcggtgcaac gcccctcggc gccggcggcg gccctttttg
     1561 cttcagaacg taaatcctcg atcacggccg gatccgttag cgatccgtgg gtgagcggcg
     1621 ccaaccatgg cctccgcgac gcccgtgccg atggcccggg cccagcgaag acccccgacc
     1681 atcttgattg aggaccacgc catgaaaccg ctcgctctgt ccctgctgct gaccagcgcc
     1741 gccctgctgc cgctgcacgt ccacgccgac accctggcgc ccgcgcccga aacgccggcg
     1801 gccgcgttcc aacgcctgtt cgcccacacc ccggggccgg tgcggccgcc cgccggtgcc
     1861 gcgcccgacg cgctgacacg cgccttcaac gaggcgctgt ggagcacccc cgatcaggtg
     1921 cccacgatcc accaggcggc gttcgatcgc gccggtctgc cgccgacggc gcaggcgccc
     1981 gaatcaccgg cggtggcgtt ccagcgcatg ctcggccaca ccccggggcc ggtgcagccg
     2041 cccgtcggcg gcgcgcccga cgtgctgacg cgcgtcttca acgacgcgct gtggagcacc
     2101 ccggccacgg cggccgccta cgcctcggcc cggcagcaca cgctggagcg cgcgcaatga
     2161 accaccggct gctgctggcg gcgcaccttg tgggtgcggc cgccctgggc gcggcgatcg
     2221 tcgcgctgtg gtggaacgac cggcgcatcg cgcgcgccgg cgactgcggc acgctggccg
     2281 gcgccggccg gcgcgcccag gcgatcgtcc agcgcctgct gctgcccggc gtgctgctgc
     2341 tggcggcctc gggcgtctgg ctgctggcgc gctactacgg ctggagcttc gtgcgcctgc
     2401 cctggctggc cgccatggcg gcgctgttcg tgttccagtc ggtctgggcc aacaccgtca
     2461 cgcgccatca cgggcagcgc ctgggccgcc tgctggccgg ccgctgcgcc accgaccggc
     2521 tcacgcccga gctggagcgg gcccggcgcg caccgctggc gcgctttggc cagcagctcg
     2581 aaccgctgct gttcgcgctc atcgtcgcct tcggcctggc gcggccgatg gactgggtgg
     2641 cgatcgccgc cggcgtcgcc gcggcggggc tgcttgcggc ggcgctgacc gcctacacgt
     2701 tgcggccacc cgcgtcgagt tccttcagca gcgcctcggt cgaggccgcg ggccgcgcct
     2761 gaagcgtggc gcccagcacc tcgcggcagc gccggtaggt ggccagcacc tgcgcgctgc
     2821 gcccgagccg gtgctgcgcg cgcatcagct gctggtagaa cggctcgacc gtggggtcgg
     2881 cgtgcagcag gcgttccagg caggcgacgg cgccttccca gtcggcggct gcgaggtgct
     2941 ggcccacatc gccgccgccc aggcgcacga agcgcgcgcg caggcgctcg cgcggggcga
     3001 tcgcccacgc cgcatcttcc tgcgccagaa aggcgccgcg gtacaggcgc agggcgcgct
     3061 cgcgatcggg gccgaccgcc gggtcgcgcg catcggggtc gatcgactca aacgcctgcg
     3121 catcgaccca gcacagctgc gggttcaggc tcagccgacc ctcctgcagc agcagcgctt
     3181 cgtgctggcc gagcaggcgg cgcagccgct gcaaggtggt ggtgaaggcg tggtgcgcat
     3241 cgtcgccttc ggcgtcgggc cacagcgcct cggccagctg ctcttcgggc acgccctggg
     3301 caccgaaggc gaccagcgcc ttcagcagcg ccagcgggcg tttctgcacc ttgccgctga
     3361 aggccagcgg ctggccgtcg agctcgatcg tgaaccggcc cagcgtgcgc aggcgcagcg
     3421 gccacggcca gtgttccaga cgcgcggccg gaccatcggg ccgcaggccg cggcgccgga
     3481 tcagcgcgcg cacgaactcg acgtcgatcc cctgctccag cgcgatgcgg caccagcgcg
     3541 ccatcggctc gtcgcgccac caggccgagt tgcaaaagcc ctgctcgcgc atgacctgga
     3601 acatgcgcgc cagcgccgcg cgctgggcgt cgtgcgcgcc gcgcgcctcg gcgaactggg
     3661 cttcgagctc gtggatgtaa tagaaggcgt tgctgcagcc gacggccgcg gccacctggg
     3721 tggcgcgctc gatctcgcgc cgcgccccgg cttcgtcgcc ggccagaaac aacgcctgcg
     3781 ccagtgccgg gcgggtgtag acctcggccc agaccacacc cgcggccagc gcgctggcca
     3841 ggccggcccg gccgtgcgcc agcatggcgg cgcggtcgtc gcgccgcatc gcctgctgga
     3901 acgagaagaa gtggtactgc acgctgtcga gcgggcggta gccgggggcg cgctcgccca
     3961 ggcgctccag aaagtcgcgc gcctgcccgg cgtcgcccga ggtgccggcg gccaccgcgc
     4021 cctgcgccac cagcatgaag tcccacacgt gggcgccggt ctcgtcggcc agcgccaggc
     4081 cgcgccgcac ggcggcgagg gcggcctcgt tgtcggcgtt catccagtgc gagatcgcca
     4141 ggatgccctc ccaggccagg cgcacgaagg ggccgacgtc gcgcgcgctg gcaaagggct
     4201 cgaggcgccg caccagttcg ttggcgcgcg ccagcttgcc gacccaccat tcgtagtaca
     4261 gcaccaggtg caccgccgcc gtcatgcgcc acgccggctc gccccggtcc tgcgccacca
     4321 gcgcgtccag ccggtgcgtc cactgcggca gctcggggtg ctgcgggcgc cggtacatca
     4381 gcagcgcaaa aattgccgcc accgcgcgca gctcggcttc acggctcgga aactgcgggt
     4441 ggcgctggcg cagggcgtcg aactcctcga tccacggatc ggccgggccg aagtcgctcc
     4501 aggcgtagag aaaggtgtcg accacgccgg cccaggacag gtattcgccg gcggcgtgac
     4561 cggcggcgcg aaaaccggtg aaggcgcttg acagcagcgt gcgcgcctgc agcgggtcgt
     4621 agggcaggcg gcacaggccg agaaagtagt ccagccaggg cgaaaccgcg cgctgcgcct
     4681 gcgggatggc caggatccat tcgcccagcg tctgaaagcg cccgctgcgc agcagcgccg
     4741 gcgcctgttc cagcaccagt ggcaccagct gcgcccagtc gccggcctcg cgcagcagcg
     4801 ccaccgccgc cggcgcgtcg ccctcggccg ccagcagggc cgcagcctgg ctgcgcaggc
     4861 ccgccagctc gtcgtcggtc agctcgcggc ggccgcgcgc cagcaggaat tcgcgaaaca
     4921 gcgggtggta ctgccaggcc tcgccgcggc ccggctcggc atggcgggcg acgaagaagt
     4981 tgcgccgttc gagctcggcc agcagcgccg ccgcgtcctg ctgcgccgtc agcgccgccg
     5041 ccgcgcgggc gctgacctgc ggcagcaggg cggtgagcaa cagaaagcgc tgcacgcgcg
     5101 catcggcctg cgccagcagc tcgccggcga agtagtcgaa caccagcggc tcggcggccg
     5161 gcgtcgcctc gtccagggtg gcggcggcgc cgcgctcgag catcagcacc agcccggcca
     5221 cccagccctg ggtgcgctgg tgcagctgct gcagcacctc gtcggccggc gcggcgcccg
     5281 ccagccgctg gcgcgccacg cccacgcatt cgtcggcgct caggcgcagc gcctcgccat
     5341 ccagcagggt gaagccttcc tgcaggcgca gccgggtcag cagcgcgggc ggctcggcgc
     5401 ggctggtgac gatcaccgcc atgcccggcg gcacctcggc caggccctcg cgcagcgcca
     5461 ggtgcaccgg cgaatcggcg tcgatcaggt gatggtcgtc cagcaccagc agcggcgtgc
     5521 cctgcaggcg cgcaaagaag cggcgaaacc agttgcgcgc gaacacttcc agcccggcca
     5581 ggtattcggc ggtgaagggc ggcagcgcgt cggcgcggcg gcgcgatccg gcggcctgcg
     5641 ccagatcgtg gaagaaggag gccgggtcgg cgtcggccgc atcgatgcgg taccagacgg
     5701 cgcgcaggcg gcgcgcctgc aggtagctcg ccaccagcgt ggtcttgccc gagccggccg
     5761 gggcggcgat ccagatcacc gtgccgccgc gccgcgcggc gtccaggcgc tcgaacaggc
     5821 gccgccgtgg ccaggcgtgc cggggcctgg gtgccgtcag cttggccagg ctgagcggcc
     5881 gccgcgcgtc gtcagtgccc atgatcgccc catccacttc accgggccgg gtcgaaccgc
     5941 cgcgtccgcc ggcccgatgg ccggacgcgg gatccacaca tggtagccac gccgggcccc
     6001 ggcgtcgagc gccggcggcg ccccggaccg agggcgattc agcctagaaa cggcagttgg
     6061 acgcgcccgc ccgtgccgtg atcggcgtgc caggcaagac gcgacgccgc cgcgggctgg
     6121 tgcccgcagg caggagcaac gccgcatggc gcgccgagcg cggtgcgggg gtgcgcagcg
     6181 tgctcaccca ggaggcgaag gccatcgcag tcgcgaaacc ctgcacgggt gcggtccgcc
     6241 cgaagttgac cagcggtcaa ctgccgtttc taggttcagc ctaggttcag ggctgccgcg
     6301 cccttcaggc aagcgccgac agctatatgt tatatagcaa ccgagcgccc cgcagaagcg
     6361 cgggcagccg aatcgtgaag tgccgcagac cggctcaggc gccggcctcg atgcgcgccg
     6421 cgatggcctg ccccatctgt acggtgctgg ccgtgccacc cagatcgggc gtgcgcgggc
     6481 ctgccgccag cacctgttcg atggcgcgca ggatggcgtc gtgcgccgcc tgctcgccca
     6541 gaaactgcag catcagcgcg gccgaccaga tcatggccac cgggttggcg atgccgcgcc
     6601 cgaagatgtc cggcgccgag ccgtgcaccg gctcgaacag gctgggaaag cgccgctcgg
     6661 ggttgaggtt ggccgaaggc gccagcccga tggtgccggt gcaggccggg cccaggtcgc
     6721 tcaggatgtc gccgaacagg ttgctggcca ccaccacgtc gaagcgctcg ggctgcagca
     6781 cgaagcgcgc cgtgaggatg tcgatgtggt gcttttgcac ggccacctgc gggtagcgcg
     6841 ccgccatggc gtcggcgcgg ccgtcccacc agggcatgct gatggcgatg ccgttgctct
     6901 tggtcgccac gtccagctgg tggcgcggac gggcggcggc ctgctcgaag gcgaacttga
     6961 gcacgcggtc ggcgccgaag gcgctcatca ccgtttcctg caccacgatt tcgcgctcgg
     7021 tgccggcata catcacgcca cccaggttgg tgtactcgcc ctcggtgttt tcgcgcacga
     7081 cgaggaagtc gatgtcgccg ggctttttac cggccaacgg gcagggcacg ccgtcgaaca
     7141 ggcgcaccgg gcgcaggttg atgtactggt cgaactcgcg ccggaatttc agcaggctgc
     7201 cccacagcga cacatggtcg ggcacggtgg ccggccagcc gacggcgccg aacagcaggg
     7261 cgtccatgcc ttgcaactgc gccttccagt cgtccggcat catctggccg gtgcgggcgt
     7321 agtagtcgca gctggcccag gcgatcgggg tctgctgcag cgcaaagccg aagcggcgtt
     7381 ggacggcgtc gatggcgcgc agcgcctcgg gcatcacttc ctggccgatg ccgtcgcccg
     7441 caatgacggc gatgcggtgg gtgcgtgggg gggtcatggg cgaatctcct gcatggcgcg
     7501 agcatagcgc cggccacgaa aaagccggcg cgtggccggc tgatctgcgc gctgcggtgg
     7561 cggtggccgc ttaccagcgc tccttgaagc gacggtaggc gctttcggcg tagtcgttgg
     7621 aggcgaactt gcgctgcgcg ccgggcgaga aatgcaggcc gtcggcgaac agccagcggt
     7681 tgtagtcggc cgaggccacg gtggcggcgg tgcaggtgct ggcgtcgggc gtggtgcaga
     7741 ccgggtccag cccgttggcg agatcgaagt tctgcggctt gccgtacatc aggttgaaga
     7801 agaaggcggc gtcgaaatac agcacgtggg cgcccagatc gacgatgctc gacagcagcg
     7861 cgtcgttgaa ggccaccgac agcgcgttga tgccgtcgcg ctggttgcgc gcgcgcgccc
     7921 aggggctgtc gcccagcaac ggcacgccgg tcaccaccac atgcttgccg ccggcgctga
     7981 ccacgcggcg cacctgggtg gccagcgcct tgccggcgtc cttgacggcc tgggtggtgg
     8041 cgtcggagag gccgccggcc tcgaccgccg ccacgatgtc gtgcatgccg ccgttgatca
     8101 gcaccacgtc gtcggtgtcg gacagcgtgg tgctggccag cagggtgtcg atctgggtgg
     8161 tgacgctggg cgcgccgccg atcgggtcgg ccgcggcgat gcgcgcatgg ccctgcgcat
     8221 ggccgctgcc gccggcgctg gcggcgctga cggtgacgcc gtaatgggcc gccagctcct
     8281 gcacccagtt gttggagccg tcgttgacgg tgtagcgctt gccgctctgg ccgacgtcca
     8341 tgaagctgtc gcccacggtg agaaagcgct tgggcgccag gtccgacacc accgagccgg
     8401 agccgcaggc cgtcagcagg gccagggtgc cggcgcagcc gagcgccagg gcggcgcggc
     8461 gaaaccagga tgcgttcatg atgagtcttg tgttgcttgg aacagggtgg cgtagtttag
     8521 cggcgccgcc cgcagcgggt gtgaccgagc ccggcgcgcc ggcgtgcgct tcggccacac
     8581 gcgctcaccc acgcatgcgg cacgagggcc tctccaattc caggattccg accccatagg
     8641 ctcaggccga ggcggcggcg cgctgcaggc ggtcgcgcac gccgtcccag gccgggtcgt
     8701 cgggcggggt ttcgacccag atcaggcggg cgccagcggc gtcgaactcg cgcagcacgg
     8761 caaacagctc gcgcgcggcg gcgtcggcgc gctcgggcat gcggcgcagc agcagctggc
     8821 gcgaggcgga ggtcagcggc gcgcgcgccc acaccgcgat gtggcgcgcc tcggcgccca
     8881 gcacctgcag cgcggcgcgc agctcgcggg cgtccatcag gcgcagcctg gccgtcggtg
     8941 cgtaatgggc cgaaagcgtg cctggcgctt gcgggtcggg cgccatcagc tcttctttgg
     9001 atagcaattg ccgacccgcc aggcgctcga tgtcggcgcg cgccagctgc cccgggcgca
     9061 gcagcaccgg cgcgccgcgg gtggcgtcga tgatggtcga ttcgatgccc acgttgcagg
     9121 caccgccgtc gagcaccagc agctcgtcgc cgaactcggc cgccacatgg gccgccgtgg
     9181 tcgggctgac gcggccgaag cggttggcgc tgggcgccgc caggccggcg atgccgcgcg
     9241 ccgcgcaggc cgccagcagg gcgcgcgcca ccgggtgcgc cgggcagcgc aggccgatgc
     9301 tggggtggcc gccggcggcg gcgtcggcca cgccggggcg gcgcggcagg atcagggtca
     9361 gcgggccggg ccagcaggcg tcgatcaggc gctgggcgaa gtcgggcacg gcgctggcga
     9421 aggccggcac ggcggcggcg ccggccacgt gcacgatcag cgggtggtcg gccggccggc
     9481 ccttggtggc gaagatggcg cgcacggcgg cggcgttgcc ggcgtcggcg gccaggccgt
     9541 agacggtttc ggtgggcagg cccaccagcc ggccggcggc cagcgcgtcc accgcctggg
     9601 cgatggcctc gggacgggcg ccgtccagaa tcatccgatg gcttccatgc ccagcagggc
     9661 ggcggcctgc cgggcgctgg cgcgcaccgc ctcggtgctg gcgccggtga acgtcaggtg
     9721 gcccatcttg cggccgcgcc gcgcctcggt cttgccgtac aggtgcaggt gggcgccggg
     9781 cagttgcagc agggcgtccc agggcggggt gcgcgggcgg ccggcggcgt cgaaccacag
     9841 atcgcccagc aggttcagca tgatggccgg gctgtgctgg cgcggcgcgc gcagcggcag
     9901 gccggccagg gtgcgcacct gcagctcgaa ctgcgagacg tcgcaggcgt ccaggctgta
     9961 gtggccgctg ttgtgcgggc gcggcgccat ttcgttgacc accagggcac cgccctccag
    10021 cacgaagtac tcaacgcaca gcacgcccac atagtcgagc tggtttgcta tcttaatggt
    10081 agctgcttgc gcttgactgg caagcgcagg aggcagattt ccctcatgga cctcggtgac
    10141 ggccaggatg ccgtcgcggt gcaggttgcg ctgcaccggc aggctgacgg cctggccgtc
    10201 gcgcccgcgc gccacgatca ccgagcattc cagcgccagc ggcagccgct gctccagcac
    10261 gcagggcacg cagcccatgg actgccaggc ggcggccagc tcggcccgcg tggccacgcg
    10321 cgcctggccc ttgccgtcgt agcccaggcg cgcggtcttc aggatgccgg gcagcagggt
    10381 ggccggcacg gcggccagct cggcctcgct ggtgatggtc gcgtgggccg ctggcgccac
    10441 accgccggcg ccggcgcagc gggcgaaatg cgccttttcg gcgctgcggt cctgcgcgat
    10501 ggcgacggcg gcggcggccg gcgccaccgg ccgcgtgccg gccagctgcg ccaggctgtc
    10561 ggccggcacg ttctcgaatt cggtgctgat ggcgtgggcc cgccgggcca gctcggccag
    10621 gccggcgccg tcgcggtagt cggcctggat gaaatgctgg ctcacgcgcc cggcggggct
    10681 ttgcgggtcg gggtcgagca ccgccgtctc atagcccatg gcctgggcgg cgtgcacgaa
    10741 catgcgcccc agttgcccgc cgcccagcac acccagggtg gcgggccggc cgtcgatcag
    10801 ggtgccaggc agcagggggg gtgggatgga catggtgcgg gacaagttga aggaacccaa
    10861 catccgtacg ccgaaggcgc caagggggcg cagagggcgc aaaagattca gagattcgaa
    10921 cctggattct ctggcgtcct gtgcgtcatc gtggcgttct ctgcgtaccg gtgttcgtgg
    10981 ttcatgctgc gactcaggcc ggcggcaggg tcatggcgcg ggcggcggcg gtctgctcgg
    11041 cgcggaaggt ctgcagcctt tgcgtcagtg ccgcatcggt ggtggccagc agggcgacgg
    11101 cgaacagcgc cgcattggcg gcgccggccg cgccgatggc aaaggtggcc accggcacgc
    11161 ccttgggcat ctgtacgatg gaatgcagcg aatcgacgcc ctgcaggtgg cgcgaggcca
    11221 ccggcacgcc cagcaccggc accaccgtct tggccgccag catgccgggc agatgggcgg
    11281 cgccgccggc gccggcgatg atggcgcgca ggccgcgctg tggcgcggct tcggcgtagg
    11341 cgaacatgtc gtcgggcatg cgatgcgccg acaccacgcg cgcttcgtgc ggcacaccga
    11401 actgctcaag gatctggacg gcgtgctgca tggtgtccca gtcgctgctg gaacccatca
    11461 ccacgcccac gaggggagtc gtcatgacgc tattgcacaa tggctggtaa tccccgattt
    11521 tgcccgcaga tacccttcat gatcgacgtc accgttcaaa acttcgaagc cgaggtcatt
    11581 gccgcctcga tgcacacccc ggtgctggtg gacttctggg cgccctggtg cggcccgtgc
    11641 aagacgctgg ggccgatcct cgagcagctg gaggtcgaat acgccggccg cttcaagctg
    11701 gtcaagatcg actccgacca gcagcaggag c
//
LOCUS       DAOOOG010000071        11609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6204_length_11609_cov_30.268046, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000071 DAOOOG010000000
VERSION     DAOOOG010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6204_length_11609_cov_30.268046"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..746
                     /locus_tag="PK242_06910"
                     /pseudo
     CDS             60..746
                     /locus_tag="PK242_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(1308..1517)
                     /locus_tag="PK242_06915"
     CDS             complement(1308..1517)
                     /locus_tag="PK242_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HOZ93783.1"
                     /translation="MFGFNEEQIAWFGLTFGVSAFILYMLFIIGQLAWESKAGKFGTF
                     VLFLALAFGMVGFIAKGVIQWIIEK"
     gene            complement(1517..2380)
                     /locus_tag="PK242_06920"
     CDS             complement(1517..2380)
                     /locus_tag="PK242_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HOZ93784.1"
                     /translation="MNHTHLPPPLPPRPARGTLTLPAVRTVGLDAPLHWLALGWRDLW
                     RVGPLSLLYGALLAGFGGLLLALAADQFWLLAGAFSGFLVIAPVLATGWYALSRALER
                     GEPANAATVRKTWIRWQYSRYAVHGGYWSLVRFGLLLALAGSGWVLTSAALITALAPQ
                     PVHTPLDFLRHVVLAPGGHLFELWLMLGALMAAPLFASSVVAMPLLLDRRADVLQAVL
                     TSWQAVLNNPAPLALWAALIMLLTLLGMATVLLGLVLALPWLGHASWHAYRALVDADS
                     LPERLPPEGGA"
     gene            complement(2541..3080)
                     /locus_tag="PK242_06925"
     CDS             complement(2541..3080)
                     /locus_tag="PK242_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOZ93785.1"
                     /translation="MNLHRKHLLLASLLAATGTLALAQAPAAGDAPARPGWSERRAER
                     MDPARMQQRMAERHAQRMDKLKAELKLNPTQEGAWTQFSTAMQPPAFEPRGPGARADF
                     AKLSTPERIDLMQKRADERHARMKQRGDATKAFYAQLTPEQQQSFDRLAARGRHGAGW
                     HAGHREHRGWGPGMGPGPR"
     gene            complement(3248..3670)
                     /gene="cueR"
                     /locus_tag="PK242_06930"
     CDS             complement(3248..3670)
                     /gene="cueR"
                     /locus_tag="PK242_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HOZ93786.1"
                     /translation="MSATPASGPIGIGEAARASGVSAKMVRHYESLGLLGQVARTDSG
                     YRQYGAADVHTLRFIRRARDLGFSMAEIAELVSLWHDRSRASADVKRIAQRHLAELEQ
                     RIAALQDMRRTLDQLVCHCHGDARPDCPILDDLAGTSG"
     gene            complement(3667..3867)
                     /locus_tag="PK242_06935"
     CDS             complement(3667..3867)
                     /locus_tag="PK242_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOZ93787.1"
                     /translation="MNQTFTVTGMTCGHCEASVKRALLQLDPGAQVQIDRSQNRVEVS
                     GSQQSREALAAAIREEGYDVAA"
     gene            4002..6281
                     /locus_tag="PK242_06940"
     CDS             4002..6281
                     /locus_tag="PK242_06940"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOZ93788.1"
                     /translation="MNTTTASSTVDLGVGGMTCASCVARVEKALQKVPGVDSAAVNLA
                     TESARVHFVGDAGELGPLLRRAVRDAGYEPRAAEAALAEREGGPWAGFLPVGIGLLLS
                     APLVLPMLGMPFGWHWMPPPWVQFLLATPVQFWLGARFYRAAWHALKARSGNMELLVS
                     IGTTAAWLLSMWLWLGKSHPPGHEPHLYFEGAAVVITLVRLGKWLEERAKRQTTAAIR
                     ALHALRPEVAHLIDWDGSEKDIPVDELRAGDRLVVRPGERVPADGRLAEGATQVDESM
                     LTGEPLPVAKAVGDALTGGSINGEDRVVLRVTAAGAESVLAHIIRLVQDAQAVKAPVQ
                     RLVDQVAAVFVPVVLLIALATLLGWWLLGGTGLEVAIMRAVAVLVIACPCALGLATPT
                     AIMAGTGVAAKFGILIKDAEALEIAHRVRTVAFDKTGTLTVGQPRLLALLPAPGVDEA
                     AALRLAAGLQSGSEHPLARAVLAQAGARGLWPPAEGTAAELAELRAVPGRGTEGRVGE
                     QRVRIGSARWMAELGVDLQVFEALAQAQQAQGATVSILATQPAEAPEGTPPRAVALLA
                     FGDEPKPGAAAALAELHARGIRAVMISGDNRGAAEAMARRLGLKPEDGEVLAEVLPGD
                     KAQAVRQLRAGGAVVGMVGDGVNDAPALAAADVGMAMGSGTDVAMHAAGITLMRGDVG
                     LVPAALDISQRTVAKIRQNLFWAFFYNAAGIPLAALGYLSPVLAGAAMALSSVSVMSN
                     ALLLRRWRPASQGGVGTPG"
     gene            6422..7006
                     /locus_tag="PK242_06945"
     CDS             6422..7006
                     /locus_tag="PK242_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOZ93789.1"
                     /translation="MKHRSLEIIRDEHSSLAAMLQSMRLMVDRGPQDDPRNFFDVLRA
                     MLFYIDEFPERLHHPKETELLFPRVAAAAPEVGMAVARLDHDHEYTEKAVRDIQHLLL
                     GWELLGESRRQRFIDAFHRYVNLYLEHMRLEEQEVLPAAEKHLTEADWRELDAEFEKN
                     CDPLTGKYKPDPVYEKLFTRIVRDAPAPIGLGGS"
     gene            7184..8296
                     /locus_tag="PK242_06950"
     CDS             7184..8296
                     /locus_tag="PK242_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HOZ93790.1"
                     /translation="MKSRAAVAFKAGEPLQIVEIDVAPPKKGEVLVRITHTGVCHTDA
                     FTLSGDDPEGIFPAVLGHEGAGIVVECGEGVTSVKPGDHVIPLYTAECGECLFCKSGK
                     TNLCVAVRATQGKGVMPDGTTRFSYNGQPVYHYMGCSTFSEYTVVAEVSLAKIDPAAN
                     PEQVCLLGCGVTTGLGAVKNTAKVQPGDTVAVFGLGGIGLAVIQGAKLAQAGRIIAID
                     TNPAKFDLARTFGATDCVNPKDFDKPIQQVIVEMTGWGVDHSFECIGNVNVMRAALEC
                     AHRGWGQSVIIGVAGAGQEISTRPFQLVTGRRWLGTAFGGVKGRSELPGMVQDAMAGR
                     IQLAPFVTHTMGLADINQAFDLMHEGKSIRSVLRHG"
     gene            8373..9203
                     /gene="fghA"
                     /locus_tag="PK242_06955"
     CDS             8373..9203
                     /gene="fghA"
                     /locus_tag="PK242_06955"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HOZ93791.1"
                     /translation="MQRIEQHASFGGRQEVWRHASASTGTPMNFGIYLPPQALAGQRC
                     PVLYWLSGLTCSEQNFITKAGVQPHAAAHGLIVVAPDTSPRGPGVADDAAYDLGQGAG
                     FYLNATQAPWAAHYRMQDYVADELPALIERHFPASDARGIFGHSMGGHGALVTALRHP
                     GRYRSVSALAPIAAPTQVPWGRKALAAYLGDDPAAWAGWDAVALIATARERLPLLIDQ
                     GEADEFLPQLRPDLLRAACEAAGHPLTLRLQPGYDHSYYFIASFIGEHVAHHAGALRS
                     "
     gene            9317..9547
                     /locus_tag="PK242_06960"
     CDS             9317..9547
                     /locus_tag="PK242_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HOZ93792.1"
                     /translation="MADTWLPTLITDTPQQGFELAITLSRKGVRYTQPDPEVLHKLRP
                     VYANDAHELTAASQVIALNFQTVAAANNYWRK"
     gene            complement(9623..10750)
                     /locus_tag="PK242_06965"
     CDS             complement(9623..10750)
                     /locus_tag="PK242_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HOZ93793.1"
                     /translation="MAQTLFTATRVGAIDVASRLVMAPLTRNRAPGAIPNALMAEYYA
                     QRADPLSGAGLIVSEGTAISPQGQGYADVPGLYGSEQIDGWKQVTQAVHARGGRIVCQ
                     LWHVGRVSHHVLQPGHADPVAPSALRANAKTYIFDAQGHGHFAPTSMPRALHRHEIPG
                     IVHDYAAAARHAVQSAGFDGVEIHGANGYLLDQFLKSGANHRTDDYGGRIAHRARLML
                     EVARAVCDAVGADRVGIRLSPVTPANDIVDADPQPLFEHLMRQLAPLGLAYIHVIEGA
                     TGGPREVEGRPFDYAAMKAAYRAAGGQGAWIVNNGYDPQSAEAALASGYADLVAFGKA
                     FISMPDLAARIRRGGPYQGLERATMYGGGAHGYTDYPALAN"
     gene            10866..>11609
                     /locus_tag="PK242_06970"
     CDS             10866..>11609
                     /locus_tag="PK242_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HOZ93794.1"
                     /translation="MSPPAPPLSRLLWLALALSLGAAVSLGITRFAYALLLPVMREDL
                     AWSYTLAGAMNTANALGYLLGAIATPWLMRRWSASTVLLTGSALASVFMAASGFFTDA
                     VPLLLQRLLAGAASALVFVSGGLMAARLGAQSPQRAGLLLGLYYGGTGWGIAASALGV
                     PLVLQAAQGVAHGWAWAWWGLAALCLAATAVLWWPANALRKQELMAQGASHPQADSPQ
                     NLPWRAMALALAGYTLFGVGYIGYMTFVIA"
BASE COUNT         1660 a         3926 c         4282 g         1741 t
ORIGIN      
        1 cccgcgatcc gttcaagctc tacggttcga tcgcgctggc gcggcaggaa ctggagctca
       61 tcggtggcta cgacgagagc tttcgcggct ggggtgccga cgacgacaac gtgcgcgttc
      121 gcctggccat gaacggtgcg cgcctgctgg gttgccccca gatgaagctg ctgcacctgg
      181 cggacgaacc tcgccgcccc agtccggcat tcaatgcagc ctcggatctg aggaaatgct
      241 ccccggcgac ggcgcgtgcc aacctcgatg gggaatgggg gcggtcgttt tcccgcctgg
      301 cctggccgat cggcagccag cctggcggcc gccccgaggt tgccgcgaca gtgcctgttc
      361 ccgtggtcag caatccagcc tttccggtgc cgtcccgcca gcgctgcccg gtctgtggtc
      421 gtttcaccca gttgcagcag gcgctgtcct tctgccttcg ctgtgtgccg cagccgcacg
      481 accaggtgtc ggacgcgccg cgcctgctgt gcgtcatgca ggtccgcaat gaagcgcgcg
      541 ttctgccggg ctgcttcgag catctgcgcg ggtatgtgga tggtttcatc gtgcttgacg
      601 atggctcgac cgacggcacg cgggagattg tcagccgcga acccgccttg ctggagctgc
      661 tcgacaaccc cccggcccag ccccatgtct ggaacgagcg ggacaacaag cgccggctgc
      721 tgcaggccgc gcgtcgtcac ggcgcgcaat gggtgctggt gtgcgatgcc gacgagcgct
      781 acgagatgat gttcgtcaag gggctgcgcc ctctcggcaa ggcgctcgac gcgctgggtt
      841 ttcagatgct gacgctggcg cgtcgcgaac tgtgggatcg accggaccag taccgcatcg
      901 atggccggtg gttgagcaag aacgatgtcc ggctgctgaa ggtgccggag gtcatcgcct
      961 tcgagcgcac ggcgcagttg cacggctcat gggtgccgga cgcgttgctg gaggcgagcc
     1021 ggcaggcgcg ccgccacgcg gtgctcaact accacatgta tcacctgaag atgattcacg
     1081 aagccgaccg gatccggcgg cgtgatctgt acaagtcgct ggatccggac ggccggttcc
     1141 aggccgaggg ctacgactat ctgaccgaag ccggtgatga actcgaactg gcgcccatca
     1201 ccgaagatcg ctggtacgac cgtcgtaccc tgccggccga tctgcaggcc atgctcgaac
     1261 ggtcaacgcc cgtgggcagc ccgacgctgc gtgccaccgc atggcgctca cttttcgatg
     1321 atccactgga tcacgccctt ggcgatgaag ccgaccatgc cgaaggccag cgccagaaac
     1381 agcacgaagg tgccgaactt gccggctttg gactcccacg ccagctggcc gatgatgaac
     1441 agcatgtaga ggatgaaggc gctgacgccg aaggtcaggc cgaaccaggc gatctgctcc
     1501 tcgttgaagc cgaacatcag gcgcctcctt cgggcggcag gcgctcgggc aggctgtcgg
     1561 cgtcgaccag cgcgcggtag gcgtgccagc tggcatggcc cagccagggc agggccagca
     1621 ccagaccgag cagcacggtg gccatgccca gcagggtcag cagcatgatg agcgcggccc
     1681 acagggccag cggcgcgggg ttgttcagca ccgcctgcca gctggtcagc accgcctgca
     1741 gcacgtcggc acgccggtcc agcagcaacg gcatggcgac cacgctggag gcgaacagtg
     1801 gcgccgccat cagggcgccc agcatcagcc acagctcgaa caggtggccg ccgggcgcca
     1861 gcaccacgtg gcgcagaaag tcgagcggcg tgtgcaccgg ctgcggcgcc agcgcggtga
     1921 tgagcgcggc cgaggtcagc acccagccgc tgccggccag cgccagcagc aggccgaagc
     1981 gcaccaggct ccagtagccg ccatgcacgg cgtagcgcga gtactgccag cgtatccagg
     2041 tcttgcgcac ggtggcggcg ttggccggct cgccgcgctc gagcgcgcgc gacagcgcgt
     2101 accagccggt ggccagcacc ggtgcgatca ccagaaagcc ggaaaaagcc ccggccagca
     2161 gccagaactg gtcggcggcc agcgccagca gcagcccacc gaagccggcc agcagggcgc
     2221 cgtacagcag gctgagcggg ccgacgcgcc acaggtcgcg ccagcccagg gccagccagt
     2281 gcagcggtgc gtccaggccg accgtgcgca cggccggcag ggtcagggtg ccgcgcgccg
     2341 ggcgcggcgg cagggggggt gggaggtggg tatggttcac gcgcagcagc ttaagggcag
     2401 tgggtcgccc gtgccagcct ggcggtgggc gacgacatca aaaagtatag ctgctggcgc
     2461 ttgatggaca agcgctgaat gccattctga cgtggaacgg cagaacccgc cggcgggcgc
     2521 cggcgggcgg tgcgccggcg tcagcgcggg cccggcccca tgcccgggcc ccagccgcgg
     2581 tgttcgcggt ggccggcgtg ccagccggcg ccgtggcggc cgcgcgcggc gaggcgatcg
     2641 aaactctgct gctgctcggg cgtgagctgg gcgtagaagg ccttggtggc gtcgccgcgc
     2701 tgcttcatgc gggcgtggcg ttcgtcggcg cgcttttgca tcaggtcgat gcgctcgggc
     2761 gtgctgagct tggcgaagtc ggcgcgcgcg ccggggccgc gtggctcgaa cgccggcggc
     2821 tgcatggcgg tgctgaactg cgtccaggcg ccttcctggg tcgggttgag cttgagttcg
     2881 gccttcagct tgtccatgcg ctgggcgtgg cgctcggcca tgcgctgctg catgcgggcc
     2941 gggtccatgc gttcggcgcg gcgctcgctc cagccggggc gggccggggc gtcaccggcg
     3001 gcgggtgcct gggccagggc cagggtgccg gtggctgcca gcaggctggc cagcagaagg
     3061 tgtttgcggt gcagattcat gcgacggtcc tttcggttgc gggacccgcg cgggcgggtc
     3121 gggccgagcc ggggcgggat gccgcggcgt tccggttgcc aggcagtgtg cgccgcccat
     3181 gtgtcggccg cttggcccgc gcatgtcggt ttgtaaagct gtgcgaagtc cggccccggc
     3241 ggccggttca gccgctggtg ccggccaggt cgtcgaggat cgggcagtcg gggcgcgcgt
     3301 cgccgtggca gtggcacacc agctggtcca gcgtgcgccg catgtcctgc agggcggcga
     3361 tgcgctgctc cagctcggcc agatggcgct gggcgatgcg cttgacgtcg gcgctggcgc
     3421 ggctgcggtc gtgccagagg ctgaccagct cggcgatttc ggccatggaa aagcccagat
     3481 cgcgcgcgcg gcggatgaag cgcagggtgt ggacgtcggc cgcgccgtac tggcggtagc
     3541 cgctgtcggt gcgcgccacc tggcccagca ggcccagcga ttcatagtgg cgcaccatct
     3601 tggccgacac gcccgaggcg cgcgccgcct cgccgatgcc gatcgggccg ctggcgggcg
     3661 tcgcgctcat gccgccacgt cgtagccttc ttcgcggatg gcggcggcca gggcctcgcg
     3721 cgattgctgg ctgccgctga cttcgaccct gttctggctg cggtcgattt gcacctgggc
     3781 gccggggtcg agctgcagca gcgcgcgctt gacgctggcc tcgcaatggc cgcaggtcat
     3841 gccggtgacg gtgaaggtct ggttcatgga atgctccgtt caaaaaacgc catgatgaac
     3901 cttgccatga tggtaatgtc aagggctttc gggagggcgc gaccatccct acgcagagga
     3961 cgcttgacat tgccatcatg gcagggttca gactggcggg catgaacacc accactgctt
     4021 cttccacggt cgatctgggt gtcggcggca tgacctgcgc cagctgtgtg gcgcgcgtcg
     4081 aaaaggcgct gcagaaggtg cccggcgtcg acagcgccgc ggtcaacctg gccaccgaat
     4141 cggcgcgcgt gcacttcgtc ggcgacgccg gcgagctggg cccgctgctg cgccgcgcgg
     4201 tgcgcgacgc cggctacgag ccgcgcgccg ccgaggccgc gctggccgag cgcgagggcg
     4261 ggccctgggc cggctttctg ccggtcggca tcggcctgct gctgtcggcg ccgctggtgc
     4321 tgcccatgct gggcatgccg ttcggctggc actggatgcc gccgccctgg gtgcagtttc
     4381 tgctggccac gccggtgcag ttctggctgg gcgcgcgctt ttaccgcgcc gcctggcacg
     4441 cgctgaaggc gcgcagcggc aacatggagc tgctggtgtc gatcggcacc acggcggcct
     4501 ggctgctgtc gatgtggctg tggctgggca agtcgcaccc gccggggcat gaaccgcacc
     4561 tgtatttcga gggtgcggcg gtggtcatca ccctggtgcg cctgggcaag tggctggaag
     4621 agcgcgccaa gcgccagacc acggccgcca tccgcgcgct gcacgcgctg cggcccgagg
     4681 tggcgcacct gatcgactgg gatggcagcg aaaaggacat cccggtggac gagctgcgcg
     4741 ccggcgaccg cctggtggtg cgcccgggcg agcgcgtgcc ggccgacggt cggctggccg
     4801 aaggcgccac ccaggtggac gagtccatgc tgaccggcga gccgctgccg gtggccaagg
     4861 cggtgggcga cgcgctgacc ggcggctcca tcaacggcga ggaccgggtg gtgctgcgcg
     4921 tcaccgccgc cggtgccgaa agcgtgctgg cccacatcat ccgcctggtg caggacgcgc
     4981 aggcggtcaa ggcgccggtg cagcggctgg tggaccaggt ggcggcggtg ttcgtgccgg
     5041 tggtgctgct gatcgcgctg gccaccctgc tgggctggtg gctgctgggc ggcaccgggc
     5101 tggaggtggc catcatgcgc gcggtggcgg tgctggtgat cgcctgcccc tgcgcgctgg
     5161 gcctggccac gcccaccgcc atcatggccg gcaccggggt ggcggcgaaa ttcggcatcc
     5221 tgatcaagga cgctgaggcg ctggagatcg cccaccgcgt gcgcaccgtg gccttcgaca
     5281 agaccggcac cctgaccgtg ggtcagccgc gcctgctggc gctgctgccg gcgccggggg
     5341 tggacgaggc ggccgccctg cggctggccg ccggcctgca aagcggcagc gaacacccgc
     5401 tggcgcgcgc cgtgctggcg caggcgggcg cgcgcggcct gtggccaccg gccgagggca
     5461 cggcggccga gctggccgag ctgcgcgccg tgcccggccg cggcaccgaa ggccgggtgg
     5521 gggagcagcg ggtgcgcatc ggcagcgccc gctggatggc cgagctgggc gtggatttgc
     5581 aggttttcga ggccctggcg caggcgcagc aagcgcaagg cgctactgtt tcgatactgg
     5641 ccacgcaacc ggccgaggcc cctgaaggca ccccgccgcg ggccgtggcc ctgctggcct
     5701 ttggcgacga gcccaagccc ggcgccgccg ccgcgctggc cgagctgcac gcgcgcggca
     5761 tccgcgccgt catgatttcg ggcgacaacc gcggcgccgc cgaggccatg gcgcgccgcc
     5821 tgggtctgaa acccgaggac ggcgaggtgc tggccgaggt gctgcccggc gacaaggcgc
     5881 aggcggtgcg gcagctgcgc gccggtggtg ccgtggtcgg catggtgggc gacggagtca
     5941 acgacgcgcc ggcgctggcg gcggccgatg tgggcatggc catgggcagc ggcaccgacg
     6001 tggccatgca cgcggccggc atcaccctga tgcgtggcga cgtcggcctg gtgccggcgg
     6061 cgctggacat ttcgcagcgc accgtggcca agatccggca gaacctgttc tgggccttct
     6121 tctacaacgc cgccggcatc ccgctggcgg cgctgggcta cctgagcccg gtgctcgccg
     6181 gcgccgccat ggcgctgtcg tcggtcagcg tgatgagcaa cgccctgctg ctgcgccgct
     6241 ggcgcccggc cagccagggc ggtgtcggca cgccgggctg accgggcctg cgccggcggc
     6301 ggtctggacg gacggtggcg ggtggcccgg ccccgacggc gcaacttgac gccgatcacg
     6361 tctggcgcgc cgggtggcca ctacactcgg gggccaagtc catagcaagg agacattcgc
     6421 cgtgaagcac cgaagcctgg aaatcatccg cgacgaacac tcgtcgctgg cggccatgct
     6481 gcagtccatg cgcctgatgg tcgatcgcgg gccgcaggac gatccgcgca acttcttcga
     6541 cgtgctgcgc gccatgctgt tctacatcga cgaattcccc gagcgcctgc accatcccaa
     6601 ggaaaccgag ctgctgtttc cgcgcgtggc ggccgccgcg cccgaggtcg gcatggcggt
     6661 ggcccgcctg gaccacgacc acgaatacac cgaaaaggcg gtgcgcgaca tccagcacct
     6721 gctgctgggc tgggaactgc tgggcgagtc gcgccgtcag cgcttcatcg acgccttcca
     6781 ccgttacgtc aacctgtacc tggagcacat gcggctggaa gagcaggaag tgctgccagc
     6841 cgccgaaaag cacctgaccg aggccgactg gcgcgagctg gacgccgagt tcgagaagaa
     6901 ctgcgacccg ctgaccggca agtacaaacc cgacccggtg tacgaaaagc tgttcacccg
     6961 catcgtgcgc gatgcgccgg cgccgatcgg cctgggcggc agctgaaggc ccggccgctg
     7021 cacctgccag ggccgcctgc gggcggccct gcgcatgggt ggggtatgca agggcggcgt
     7081 aggaggtttg tgccgccctg ggccataaac gctcctgccg ttgccgattc cgctgccgcg
     7141 gtcgcaggca gcgccgcccg tccgtttgcc aaggagaatc cgcatgaaat cccgcgccgc
     7201 cgttgccttc aaggctggag aacccctgca aatcgtcgag atcgacgtcg ccccgccgaa
     7261 gaagggcgag gtgctggtgc gcatcaccca caccggcgtc tgccacaccg atgccttcac
     7321 gctctcgggc gacgaccccg agggcatctt ccccgccgtg ctcgggcatg agggcgcggg
     7381 catcgtcgtc gaatgcggcg aaggcgtgac cagcgtcaag cccggcgacc atgtgatccc
     7441 gctgtacacg gccgaatgcg gcgaatgcct gttctgcaag agcggcaaga ccaacctctg
     7501 cgtggccgtg cgcgccaccc agggcaaggg cgtgatgccc gatggcacga cacgcttttc
     7561 ctacaacggc cagcccgtct accactacat gggctgttcc actttcagcg aatacaccgt
     7621 ggtggccgag gtgtcgctcg caaagattga ccccgcggcc aaccccgagc aggtgtgcct
     7681 gctgggctgc ggcgtaacca cgggcctggg cgccgtgaag aacaccgcca aggtgcagcc
     7741 cggcgacacc gtcgcggtgt ttggtctggg cggtattggc ctggctgtaa tccagggcgc
     7801 gaagctcgcc caggccgggc gcatcatcgc catcgacacc aatccggcca agttcgacct
     7861 ggcgcgcacc tttggcgcga ccgactgcgt caaccccaag gactttgaca agccgattca
     7921 gcaggtcatc gtggagatga ccggctgggg tgtcgatcat tccttcgaat gcataggcaa
     7981 cgtgaatgtg atgcgcgcgg cgctcgaatg cgcgcaccgt ggctggggcc agagcgtgat
     8041 catcggcgtg gcgggcgcgg ggcaggagat ctccacccgc cccttccagc tcgtcacggg
     8101 ccggcgctgg ctgggcacgg cattcggcgg ggtcaagggc cgcagcgaac tgcccggcat
     8161 ggtgcaggac gccatggccg ggcgcatcca gcttgcgccc ttcgtcacgc acaccatggg
     8221 cctggccgac atcaaccagg ccttcgacct catgcacgag ggcaaatcga ttcgctcggt
     8281 gctgcgccac ggctgagtca tcacataaca aatcgggcca cggcgcttgt ctgacaagcg
     8341 ccaacagcta tcgaaatagg agtattcggg gcatgcaacg gatcgagcag cacgccagct
     8401 tcggcggccg tcaggaggtc tggcggcacg ccagcgcgag caccggcacg ccgatgaact
     8461 tcggcatcta tctgcccccg caggcgctgg cgggccagcg ctgcccggtg ctgtactggc
     8521 tgtcggggct gacctgcagc gagcagaact tcatcaccaa ggccggggtg cagccgcacg
     8581 ccgccgcgca cggcctgatc gtggtggcgc ccgacaccag cccgcgcggg cccggcgtgg
     8641 ccgacgacgc ggcctacgac ctgggccagg gcgcgggctt ttatctgaac gccacccagg
     8701 cgccgtgggc ggcgcattac cgcatgcagg actatgtggc cgacgagctg ccggcgctga
     8761 tcgagcggca ctttcctgcc agcgacgcgc gcggcatctt cggccacagc atgggcgggc
     8821 acggcgcgct ggtgacggcg ctgcgccacc cggggcgcta tcgcagcgtg tcggccttgg
     8881 cgccgattgc cgcgccgacc caggtgccct ggggccgcaa ggcgctggcg gcgtacctgg
     8941 gcgacgatcc ggcggcctgg gccggctggg acgccgtggc cctgatcgcc accgcgcgcg
     9001 agcgcctgcc gctgctcatc gaccagggcg aggcggacga attcctgccg cagctgcgcc
     9061 ccgatctgct gcgcgccgcc tgcgaggccg ccggccaccc gctgacgctg cgcctgcagc
     9121 cgggttacga ccacagctac tacttcatcg ccagcttcat cggcgagcat gtggcgcacc
     9181 acgccggcgc gctgcgcagc tgatccccag gcgccgtgcg gtcatggcgc cgggtgcggc
     9241 cggcgcgcgc gcggcgtggg gacaatgcag gcgccgatcc cggcacccac cacaccaccc
     9301 acgaaggatc cgaaccatgg ccgacacctg gctgcccacc ctcatcaccg acaccccgca
     9361 gcagggtttt gaactggcga tcacgctcag ccgcaagggc gtgcgctaca cccagcccga
     9421 ccccgaggta ctgcacaagc tgcgcccggt gtatgccaac gacgcgcacg agctgaccgc
     9481 cgcctcgcag gtgatcgcgc tcaacttcca gaccgtcgcc gccgccaaca actactggcg
     9541 caagtgagcg gcgccgcgct ggtcgtttta cccatgaacg cggttttgca atgtccaatt
     9601 tcgggctgcc gaggccaaag gttcaattcg ccagcgccgg atagtcggtg tatccgtgcg
     9661 cgccaccgcc gtacatggtg gcgcgctcca gcccctggta ggggccgccg cggcggatgc
     9721 gggcggccag gtcgggcatg ctgatgaagg ccttgccgaa cgccaccagg tcggcgtagc
     9781 cgctggccag ggcggcctcg gccgactgcg ggtcgtagcc gttgttgacg atccaggcgc
     9841 cctgaccgcc ggcggcgcgg taggcggcct tcatggcggc gtagtcgaac ggtcggccct
     9901 cgacctcgcg cgggccgccg gtggcgcctt caatcacgtg gatgtaggcc aaacccagcg
     9961 gcgccagctg gcgcatcagg tgctcgaaca gcggctgcgg gtcggcgtcg acgatgtcgt
    10021 tggccggtgt gacgggcgac aggcggatgc cgacgcggtc ggcgccgacg gcgtcgcaga
    10081 cggcgcgggc gacttccagc atcaggcggg cacggtgggc gatgcggccg ccgtagtcgt
    10141 cggtgcggtg gttggcgccg ctcttgagga actggtccag caggtagccg ttggcgccgt
    10201 ggatttccac gccgtcgaag ccggccgatt gcacggcatg gcgcgcggcg gcggcgtagt
    10261 cgtgcacgat gccggggatt tcgtggcgat gcagcgcgcg cggcatgctg gtgggggcga
    10321 aatgaccatg gccctgggcg tcgaagatgt aggtcttggc gttggcgcgc agcgccgaag
    10381 gcgccaccgg gtcggcgtgg ccgggttgca gcacatggtg cgacacgcgc cccacatgcc
    10441 agagctggca gacgatgcgg ccgccgcgcg catgcacggc ctgcgtcacc tgcttccagc
    10501 cgtcgatctg ttcgctgccg tacaggcccg gaacgtcggc gtagccctgg ccctgcgggc
    10561 tgatggccgt gccttcgctg acgatcaggc cggcgccgct cagcgggtcg gcgcgctggg
    10621 cgtagtactc ggccatcagc gcattgggaa tggccccggg cgcgcggttg cgggtgagcg
    10681 gcgccatgac gagccggctg gcgacgtcga tggcgccgac gcgggtggcg gtgaacaggg
    10741 tctgggccat gggtgggtct cgcggtcagg ggctgcctgg gccgattgtg cgacaggggg
    10801 gcgcgagtgc gctgcctgtc cccgccgggc cggcaggcag ggcctgcgcg gcgcacaatc
    10861 gccccatgag cccgcccgcc ccgcccctgt cgcgcctgct gtggctggcg ctggcgctgt
    10921 cgctgggcgc ggcggtgtcg ctgggcatca cgcgctttgc ctatgcgctg ctgctgccgg
    10981 tgatgcgcga ggacctggcc tggagctaca ccctggccgg cgccatgaac accgccaacg
    11041 cgctgggcta tctgctgggg gcgatcgcca cgccctggct gatgcggcgc tggagcgcct
    11101 cgacggtgct gctgacgggc agtgcgctgg ccagtgtgtt catggcggcg tcgggtttct
    11161 tcaccgacgc cgtgccgctg ctgctgcagc gcctgctggc cggcgcggcc agcgcgctgg
    11221 tgttcgtctc gggcggtctg atggcggcgc ggctgggggc gcagtcgccg cagcgggccg
    11281 ggctgctgct ggggctgtac tacggtggca cgggctgggg catcgcggcg tcggcgctgg
    11341 gcgtgccgct ggtgctgcag gccgcgcagg gcgtggcgca cggctgggcc tgggcctggt
    11401 gggggctggc ggcgctgtgc ctggcggcca cggcggtgct ctggtggccg gccaatgcgc
    11461 tacgaaaaca ggagctaatg gcgcaaggtg caagccatcc acaggccgat tcaccccaaa
    11521 acctgccatg gcgcgcgatg gcgctggcgc tggccggcta cacgctgttc ggcgtcggct
    11581 acatcggcta catgaccttc gtcatcgcg
//
LOCUS       DAOOOG010000072        11541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6256_length_11541_cov_30.254571, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000072 DAOOOG010000000
VERSION     DAOOOG010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6256_length_11541_cov_30.254571"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PK242_06975"
     CDS             complement(<1..205)
                     /locus_tag="PK242_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93795.1"
                     /translation="MPDWQVSAVGHVERFTQALLRGDARVEAREFDLARAEVARTGRP
                     ELVARVELTRCAARVASLDFAPCT"
     gene            complement(283..1317)
                     /locus_tag="PK242_06980"
     CDS             complement(283..1317)
                     /locus_tag="PK242_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOZ93796.1"
                     /translation="MTEAEPSAVPTPPPAPDAAVADVARVPLRGLELRAALLLILMAL
                     LVGAAVLYLMWVRGAFEATQKLYLSADDSEGVVVGMDMSFSGFPIGRVSRIELAEQGS
                     VRIHVDVPVKSARWLRTSSVFTLEKGLVGAARLRAFSGILDDPPLPPGAERIVLRGDV
                     SAEIPKMVADARDVLLNLNRLTGEQSALYATLGEVQRFTRRLNSSQGGLMAAVTGNEA
                     DARRVSELLQRTAQLVKHLDAVTQRVDGVVQRADRQVLGQDGLLTDAQASVRQLGALL
                     QDVRQSVVKVDAVLTEAQAVAGNAREASTDLGALRADVEASLRKLDTLITELNRKWPF
                     APRDKELRLP"
     gene            complement(1324..2127)
                     /locus_tag="PK242_06985"
     CDS             complement(1324..2127)
                     /locus_tag="PK242_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ93797.1"
                     /translation="MTTGYSLYGWLAGLGRGLLRWLLGWWAVLFTGAQVAVLAFSPSS
                     YGPEARGAVLRHIYHAAGPGLPGFTTLMTLFNVVLIRILVVTAFSYGLTQYALDAVVR
                     VLVLELIPLIAALFVAVEYTIPGGSELYKLRRAGALQLMRASGREPLSHEVLPRVLAG
                     MFAVLLLALLSALISLVLAYVAVHGFTLAGLPSYNHAVGNIFNPVVSMIFCMKTLLFA
                     LAVALLPVANALRDIPRRGSRTSVELQGLVQMSVLMLVIEIASLIGNYY"
     gene            2315..3796
                     /gene="phaZ"
                     /locus_tag="PK242_06990"
     CDS             2315..3796
                     /gene="phaZ"
                     /locus_tag="PK242_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HOZ93798.1"
                     /translation="MLYQIYEAQRSLLEPFADMADAASKLYGNRHTLLGQMPMAQRIS
                     AGFALFHRFGKDYEKPEFGIRTVDVDGVSVAIDERVEIDKPFCELRRFKRFSDDPPTL
                     EKLKRQPPVLIVAPLSGHYATLLRDTVRTMLEGHKVYITDWKNARMVPLSEGEFHLDD
                     YVNYVQDFIRHLQKHYGNCHVISVCQPTVPTLAAVSLMASRGEKTPISMTMMGGPIDA
                     RKSPTQVDDLAVKRSYEWFENNVIYRVPPNYPGAGRRVYPGFLQHAGFIAMNPDRHTS
                     SHYDYFRDLIKGDMSSAETHRKFYDEYNAVLDMDAPYYLQTIQTVFQDFKLVHGTWDV
                     RSPEGKLERVRPQDITGTCLLTVEGELDDISGVGQTKAAHALCKGIGRREGDHIEVEG
                     AGHYGIFSGRRWRTTVYPQVKAFIGELHAKSGLPPTFAPASSPAPAPAQRALNGQRAA
                     PRPASNGSATPAPRPRAASQPKAKATASSSAAGRRGKSAPRAA"
     gene            3863..4540
                     /gene="rsxB"
                     /locus_tag="PK242_06995"
     CDS             3863..4540
                     /gene="rsxB"
                     /locus_tag="PK242_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HOZ93799.1"
                     /translation="MNPPPAKPDALAIRLLAALPQTQCTRCGYPDCAAYAQAIAAGAA
                     PINRCPPGGAEGVARLAALTGQPVLPLDPEHGTEGPRGVAWIDEDWCIGCTLCIAACP
                     VDCILGGNKRMHTVIEAECTGCELCVPACPVDCIHMEAVSGSLTGWAAWSAAEAESAR
                     MRYEFTSWRRRRDQRTPPLDSEEPRPSADSAPPSVATAQPSEAERKRRLIDAALARAR
                     QQRPPPR"
     gene            complement(4568..6121)
                     /locus_tag="PK242_07000"
     CDS             complement(4568..6121)
                     /locus_tag="PK242_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOZ93800.1"
                     /translation="MRTALVADHPPLTGASRVLGTLALSAATFMNVLDSTIANVSLPA
                     IAGDLGVSPVQGTWVITSFAVANAIGVPLTGWLTQRFGQVRLFVASVLLFVLTSWLCG
                     LAHNMTTLIALRALQGLVAGPMIPLSQTLLLASYPRALAGLAMALWSITTLVAPITGP
                     LLGGWITDNASWPWIFYINVPVGLLAAVVTWAIYRERESPTRRLPIDVVGLVLLVLWV
                     GALQLMLDKGKELDWFHSPQIVAFAVVAVVGLAFFLAWELTDEHPVVDLSLFARRNFW
                     SGALATAVGYGVFIGNVVILPLWLQQFMGYTATQAGMILAPVGLLAIVLAPLVGLSIN
                     RVDPRLYVTGSFLVFALVLWMRSRFTTLTDFDTIMIPTLIQGVAMAGFFIPLVTITLS
                     GLEPSRIPAAAGLSNFTRITAGAFGVSISATLWEDRSAMHHAHLVESINTGNPAAMQT
                     LDQLRAGGLSAEQSLGVVNNLVTQQAFMLSTQELFYGSALLFLLMIPLVWLARRPAGA
                     AAGDAGGAH"
     gene            complement(6198..7460)
                     /locus_tag="PK242_07005"
     CDS             complement(6198..7460)
                     /locus_tag="PK242_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ93801.1"
                     /translation="MDDHDTPAHAAPQGADPGPAAAGRRRARRKPALLSVTALVLLAG
                     LAWGAYDWLVLSHYQETDNAYVQGNVVQITPQQGGTVTAIYADETDRVSAGQPLVRLD
                     GADAQVALAQAEAALGQAVRQVRTLYVNNHSLAAQVRLREADVAKARSQLATAEQDVA
                     RRQGLVAGGAVSGEELAHARAQVSAAQSTLTAAQAAASAAREQLASNQALTEGTPVAE
                     HPSVLTAAAKLREAWLAAHRTSIPAPVSGYVGKRSVQLGQRVAPGAPLMTVVALEQVW
                     VDANFKENQLRQIRVGQPVRLTADVYGGKAVFDGKVAGLGIATGAASALLPAQNATGN
                     WIKVVQRVPVRIALDPEQLKAHPLRVGLSMLARVDVRDQQGPALAEAPRGEPLAQTAV
                     FADAGQGAEQRVREIIARNLGRQVGQGS"
     gene            complement(7513..8982)
                     /locus_tag="PK242_07010"
     CDS             complement(7513..8982)
                     /locus_tag="PK242_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOZ93802.1"
                     /translation="MTSQRPPRPAARGLLAAALVLLALAGCADTQGIRPQARLTDAAA
                     LGLPAGQDLAPAPDWWRALGDAQLDSLVLQALEGNPNLRVAAARVAKARAGELAARAA
                     SLPQVGGAADLNRQSFSSHYIYPPPLGGTVQTLANLQLNGGWELDFFGKHAGALDAAI
                     GQTRAAQAQADAARVLLAANVVRAYVQWAHVNELHQLAERTLAQRQQMTQLVQQRTQA
                     GLDNQVSLRQNESSRADTRTSLAALAQQQQAARQALAALLGQPRLADAVAAPRLAQLK
                     TLAVPAELHADWLARRADISAARWRVEAARGEVQVARAQFYPNINLAGFLGFQSLGFD
                     NLLKGGSFQWGVGPALRLPIFEAGKLRAHLSASSAEVDAAIESYNAAVIDAVREAHDQ
                     AHAVQSLARQQAEQAQAQAAAEAAWDLARQRYQAGLGTYLQVLGAETAVLAQRRQQLD
                     LAAQALGAQIGLTQAMGGGWQPSATVSSPGTTTVSVAPH"
     gene            complement(8979..9509)
                     /locus_tag="PK242_07015"
     CDS             complement(8979..9509)
                     /locus_tag="PK242_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOZ93803.1"
                     /translation="MTASTAPPFSAAGPATSPAAPLLQPETFTPDQSIMYLLKRVLVG
                     LSQATTRELAMEDSSLPQWLPLFKLWRADAHTVAELARQCSIDVSTMTRMLDRLEQRD
                     LVRRERSQADRRVVQLRLTPAGQAVAERLPAVMCAVYNRALAGFSPDEWALLQALLHR
                     LVANAEAMAQQGKAPQ"
     gene            complement(9624..10679)
                     /locus_tag="PK242_07020"
     CDS             complement(9624..10679)
                     /locus_tag="PK242_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOZ93804.1"
                     /translation="MTYIAKPRLHHPSLTKNQVGYTRRDYEGKVSTLCAGCGHDSVSA
                     AIVQACWELDIEPHRVAKLSGIGCSSKTPDYFLGASHGFNTVHGRMPSVLTGANLANR
                     DLLYLGVSGDGDSASIGLGQFAHAMRRNVNMVYIVENNGVYGLTKGQFSATADKGSQS
                     KKGVVNADSPVDLVGLAMQLGASFVARSFSGDKAQLVPLIKAAIGHGGAAFIDVISPC
                     VTFNNHAGSTRSYDWVREHNEAVSRIDFITGRDEITATIAPGEVVDVEQHDGTILRLR
                     KLHTDYDPTDRVAAMAYMQAHAAQGELVTGLLYLEGEAGDLHAAQHTSARPLNSLDTA
                     DLCPGAAALERLNAGWR"
     gene            complement(10676..>11541)
                     /locus_tag="PK242_07025"
     CDS             complement(10676..>11541)
                     /locus_tag="PK242_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOZ93805.1"
                     /translation="STGMPTRTQQSDILACAYASHGDTKHVLLLPEDPRECFEHTACA
                     LDLADRLQTPVFVMTDLDIGMNQRLCEPLAWDDARRYDRGKVMSAEDLEQKDFGRYKD
                     VDGDGIPWRTLPGTHPTRGSYFTRGTTRDAYARYSERGPDYIYNVQRLLKKFATAATL
                     VPQPVPRPAARRTRLGVIFYGSTSPAMHEALDELAGQGIHLNALRLRAFPFPASVKEF
                     IDAHETVFVVEQNRDAQMRSLLINELEINPAKLPRVLHYDGTPITARFITQAITDHVH
                     EAVVAPQENPS"
BASE COUNT         1766 a         4090 c         4038 g         1647 t
ORIGIN      
        1 cggtgcaggg cgcgaagtcc aggctggcca cccgggcggc gcagcgcgtc agctcgacgc
       61 gcgccaccag ctcggggcgg ccggtgcgcg ccacctcggc gcgcgccaga tcgaattcgc
      121 gcgcctccac ccgcgcgtca ccgcgcagca aggcctgggt gaaacgctcc acgtggccca
      181 ccgcgctgac ctgccaatcc ggcatcgatg ggccgctggc gcagccccag agagcggcac
      241 cagctatcac aacaatagca aatcgaatca tgcaccgggc ggtcatggca gccgcagctc
      301 cttgtcccgg ggagcgaacg gccatttgcg gttcagctcg gtgatcagcg tgtcgagctt
      361 gcgcaggctg gcttcgacgt cggcacgcag cgcgcccagg tcggtgctgg cttcgcgcgc
      421 gttgcccgcc acggcctgcg cctcggtcag cacggcatcg accttgacca cgctctggcg
      481 cacgtcctgc agcagcgcgc cgagctggcg cacgctggcc tgggcgtcgg tgagcaggcc
      541 gtcctggccc agcacctgcc ggtcggcgcg ctgcaccacg ccatcgacgc gctgcgtcac
      601 ggcgtccagg tgcttcacca gctgcgcggt gcgctgcagc agctcgctga cgcggcgcgc
      661 gtcggcctcg ttgccggtga ccgccgccat caggccgccc tggctgctgt tcaggcgccg
      721 ggtgaagcgc tgcacctcgc ccagggtggc gtacagcgcc gactgctcgc cggtcaggcg
      781 gttgaggttg agcagcacgt cgcgggcgtc ggccaccatc ttggggattt cggccgacac
      841 gtcgccgcgc agcacgatgc gctcagcccc gggcggcagc ggcgggtcgt ccagaatgcc
      901 gctgaaggcg cgcagccgcg ccgcgccgac caggcctttt tccagcgtga agacgctgga
      961 ggtgcgcagc cagcgcgccg acttgaccgg cacatcgacg tggatgcgca ccgagccctg
     1021 ctcggccagc tcgatgcggc tgacgcggcc gatcgggaag cccgagaagc tcatgtccat
     1081 gccgaccacc acgccctcgg agtcgtcggc gctcaggtac agcttttgcg tggcctcgaa
     1141 agcgccgcgc acccacatca ggtacagcac ggcggcaccg accagcagcg ccatcaggat
     1201 cagcagcagc gcggcgcgca gctccagccc gcgcagcggc acgcgcgcca cgtcggccac
     1261 ggcggcgtcc ggcgcgggtg gcggggtggg cacggcggaa ggctcggctt cagtcatcgg
     1321 gcatcaatag taattgccga tcagcgaggc gatctcgatc accaacatca gcaccgacat
     1381 ctgcaccagc ccctgcaact cgacgctggt gcgcgagccg cggcgcggaa tgtcgcgcag
     1441 tgcgttggcc accggcagca gggccacggc cagcgcgaac agcagcgtct tcatgcagaa
     1501 gatcatcgac accaccgggt tgaagatgtt gcccacggcg tggttgtacg agggcaggcc
     1561 cgccagcgtg aagccatgca cggcgacgta ggccagcacc agcgagatca gcgcgctcag
     1621 cagcgccagc agcagcaccg cgaacatgcc ggccagcacg cgcggcagca cctcgtggct
     1681 gagcggctcg cgcccgctgg cacgcatgag ctgcagcgcc ccggcgcggc gcagcttgta
     1741 cagctcggag ccgccgggga tggtgtattc caccgccacg aacagcgcgg cgatcagcgg
     1801 gatcagctcc agcaccagca cgcgcaccac ggcgtccagg gcgtactgcg tcaggccata
     1861 ggaaaaggcg gtgaccacca ggatgcggat cagcaccacg ttgaacaagg tcatcagggt
     1921 ggtgaagccg ggcaggcccg gcccggccgc gtggtagatg tggcgcagca cggcgccgcg
     1981 cgcctcgggg ccgtagctgg agggcgaaaa cgccagcacc gccacctggg caccggtgaa
     2041 cagcaccgcc caccagccca gcagccagcg cagcaagccc cgcccaagcc ccgccagcca
     2101 gccgtacagc gaatacccgg tggtcatggc gccatgatac ccacagccct cgctggcaaa
     2161 gccgaaccgg cgacggcaac ccccaggctg tcgccggcgc gacgacagtc acgccagcgc
     2221 gtcgcccggt gtgcattttt aggggcggca caccatgcaa tttcggcccg gctgcgttat
     2281 attgcagtgc aacacgaggg ttacacataa gcatatgctc tatcaaatct atgaggcgca
     2341 gcgctcgctg ctcgagccct tcgccgacat ggccgatgcg gcctccaagc tgtacggcaa
     2401 ccgccacacg ctgctgggcc agatgcccat ggcgcagcgc atttcggccg gttttgcgct
     2461 gtttcaccgc ttcggcaagg actacgaaaa gcccgagttc ggcatccgca cggtcgacgt
     2521 cgacggcgtg tcggtggcga tcgacgagcg cgtcgagatc gacaagccgt tttgcgagct
     2581 gcgccgcttc aagcgcttct cggacgatcc gccgacgctg gaaaagctca agcgccagcc
     2641 gccggtgctg atcgtcgccc cgctgtcggg ccattacgcc accctgctgc gcgacaccgt
     2701 gcgcaccatg ctggagggcc acaaggtcta catcaccgac tggaagaacg cgcgcatggt
     2761 gccgctgtcg gagggcgagt tccacctaga cgactacgtc aactacgtgc aggatttcat
     2821 ccgccacctg cagaagcatt atggcaactg ccacgtcatc agcgtctgcc aacccaccgt
     2881 gccgacgctg gctgcggtgt cgctgatggc ctcgcgcggc gagaagaccc ccatcagcat
     2941 gaccatgatg ggcggcccga tcgacgcccg caagtcaccc acccaggtcg acgacctggc
     3001 ggtcaagcgc agctacgagt ggttcgagaa caacgtcatc tatcgcgtgc cgcccaacta
     3061 ccctggcgcc ggccgccgcg tgtatccggg ttttctgcag cacgccggct tcatcgccat
     3121 gaatccggac cgccacacca gcagccacta cgactatttc cgcgacctga tcaagggcga
     3181 catgtcgagt gccgagacgc accgcaagtt ctacgacgag tacaacgccg tgctcgacat
     3241 ggacgcgccg tattacctgc aaaccatcca gaccgtgttc caggacttca agctggttca
     3301 cggcacctgg gacgtgcgct cgcccgaagg caagcttgag cgcgtgcggc cgcaggacat
     3361 caccggcacc tgcctgctga cggtggaagg cgagttggac gacatctccg gtgtcggcca
     3421 gaccaaggcc gcgcacgccc tgtgcaaggg catcgggcgg cgcgagggcg atcacatcga
     3481 ggtcgagggc gcgggccact acggcatttt cagcgggcgc cgctggcgca ccacggtcta
     3541 cccgcaggtc aaggccttca tcggcgagct gcacgccaag tctggcctgc cgccgacctt
     3601 cgcgccggcc agctcgcccg cgccggcgcc ggcccagcgg gcgttgaacg gccagcgtgc
     3661 ggcgccacgc ccggcaagca acggcagcgc cacgccggcg ccgcgtccgc gggccgcaag
     3721 ccagccgaag gccaaggcaa ccgcctcctc cagcgccgcc ggccgccgcg gcaagagcgc
     3781 cccccgtgcc gcctgacggc caggcgcgca acgcccgcaa cgcccgccct ggcgggcgtt
     3841 gtcatttgtg ccggagccac gcatgaaccc gccgcccgcc aagcccgacg cgctggcgat
     3901 ccgcctgctg gcggcgctgc cgcagaccca gtgcacccgc tgcggctacc cggactgcgc
     3961 cgcctacgcc caggccatcg ccgccggcgc ggcgccgatc aaccgctgcc cgccaggtgg
     4021 cgccgagggc gtggcgcggc tggccgccct caccggccaa ccggtgctgc cgctcgaccc
     4081 cgagcacggc accgaaggcc cgcgcggcgt ggcctggatc gacgaagact ggtgcattgg
     4141 ctgcacgctt tgcatcgccg cctgcccagt ggactgcatc ctcggtggca acaagcgcat
     4201 gcacacggtg atcgaggccg agtgcaccgg ctgcgagctg tgcgtgccgg cctgcccggt
     4261 cgactgcatc cacatggaag ccgtcagcgg cagcctcacc ggctgggccg cctggagcgc
     4321 ggccgaggcc gaatcggccc gcatgcgcta tgaattcacg agctggcggc gaaggcggga
     4381 ccagcgcacc cccccgctgg actctgaaga accccgccct tcagccgaca gcgccccgcc
     4441 gtctgtcgcc accgcgcagc cctcggaagc cgagcgcaag cgccgcctga tcgacgccgc
     4501 gctggcccgt gcgcgccagc agcgtccgcc cccgcgctga agggcggctg cgcccggccg
     4561 gcagccctca gtgcgcgccg ccggcatccc ccgcggcagc gccagcgggt cgcctggcca
     4621 gccacaccag cggaatcatc agcagaaaca gcagcgccga gccatagaac agctcctgcg
     4681 tcgacagcat gaaggcctgt tgcgtgacca ggttgttgac cacgcccagc gactgctcgg
     4741 cgctcaggcc gccggcccgc agctgatcga gcgtctgcat ggccgccggg ttgccggtgt
     4801 tgatcgactc gaccaggtgc gcgtggtgca tggcgctgcg gtcttcccac agggtggcgc
     4861 tgatcgacac cccgaaggcg ccggccgtga tgcgggtgaa gttggacagg ccagccgccg
     4921 ccggtatgcg cgacggctcc agccccgaca gcgtgatggt gaccagcgga atgaagaagc
     4981 ccgccatcgc cacgccctgg atgagggtgg ggatcatgat ggtgtcgaaa tccgtcagcg
     5041 tggtgaagcg cgagcgcatc cacagcacca gcgcgaacac caggaaggag ccggtgacgt
     5101 acaggcgcgg atcgacccgg ttgatcgaca gccccaccag cggcgccagc acgatggcca
     5161 gcaggcccac cggtgccagg atcatgcccg cctgggtggc cgtgtaaccc atgaactgct
     5221 gcagccacag cggcaggatg accacgttgc cgatgaacac gccgtagccg accgccgtgg
     5281 ccagcgcgcc cgaccagaaa ttgcgccgcg caaacaacga caggtcgacc accgggtgct
     5341 cgtcggtcag ctcccaggcc agaaagaagg ccaggcccac caccgccacc acggcgaacg
     5401 ccacgatctg cggcgagtgg aaccagtcca gctccttgcc cttgtccagc atcagctgca
     5461 gcgcgccgac ccacagcacc agcagcacca gccccaccac gtcgatcggc agccggcgcg
     5521 tgggcgattc gcgctcgcgg tagatcgccc aggtgaccac cgccgccagc aggccgaccg
     5581 gcacgttgat gtagaagatc cacggccagc tggcgttgtc ggtgatccag ccgcccagca
     5641 acggcccggt gatgggcgcc accagcgtgg tgatcgacca cagggccatc gccagccccg
     5701 ccagcgcgcg cgggtagctg gccagcagca gcgtctgcga cagcgggatc atcggtccgg
     5761 cgaccagccc ctgcaaggcg cgcaacgcga tcagcgtcgt catgttgtgc gccaggccgc
     5821 acagccagga agtcagcacg aacagcagca cgctggccac gaacagccgc acctggccga
     5881 agcgctgggt cagccagccg gtcagcggca cgccgatggc gttggccacg gcaaaactgg
     5941 tgatcaccca ggtgccctgc accgggctga cccccaggtc gcccgcgatg gccggcaacg
     6001 acacgttggc gatggtcgaa tccagcacgt tcatgaacgt ggccgccgac agggccagcg
     6061 tgcccagcac gcgcgaggcg ccggtcagtg gcgggtgatc ggcaacaagg gcggtgcgca
     6121 tgcggtctgg tgggctggtt tactattatt tcaggagctg ctggcgcttg tccagcaagc
     6181 gacagcacca gttttcatca ggaaccctgg ccgacctggc gccccaggtt gcgcgcgatg
     6241 atctcgcgca cgcgctgctc cgcgccctgc ccggcatcgg cgaacaccgc cgtctgcgcc
     6301 agcggctcgc cgcgcggcgc ctcggccagg gccgggcctt gctgatcgcg cacatccacc
     6361 cgcgccagca tcgacaggcc cacccgcagc ggatgtgcct tgagctgctc ggggtcgagc
     6421 gcgatgcgca ccggcacgcg ctgcaccacc ttgatccagt tgccggtggc gttctgcgcc
     6481 ggcagcagcg cgctggccgc gccggtggcg atgcccaggc ccgccacctt gccgtcgaac
     6541 accgccttgc cgccatacac gtcggcggtc agcctgaccg gctggccgac acgaatctgg
     6601 cgcagctggt tttccttgaa gttggcatcc acccagacct gctccagcgc caccaccgtc
     6661 atcagcggcg cgcctggggc cacgcgctgg cccagctgca cgctgcgctt gcccacgtag
     6721 ccggacaccg gcgccgggat gctggtgcgg tgcgccgcca gccaggcctc gcgcagcttg
     6781 gcggcggccg tcagcacgct ggggtgctcg gccaccggcg tgccctcggt cagcgcctgg
     6841 ttgctggcca gctgctcgcg cgccgcgctg gcggcggcct gggcggcggt cagcgtgctt
     6901 tgcgcggcgc tgacctgggc gcgcgcgtgc gccagctcct cacccgagac ggcgccgccg
     6961 gccaccaggc cctggcgtcg cgccacgtcc tgctcggccg tcgccagctg gctgcgcgcc
     7021 ttggccacgt cggcctcgcg cagcctgacc tgcgcggcca gcgagtggtt gttgacgtac
     7081 agcgtgcgca cctggcgcac cgcctggccc agcgccgcct cggcctgggc cagcgccacc
     7141 tgggcgtcgg cgccatccag gcgcaccagc ggctggccgg cgctgacgcg gtcggtctcg
     7201 tcagcgtaga tcgccgtcac cgtgccaccc tgctgcggcg tgatctgcac cacgttgccc
     7261 tgcacgtagg cgttgtcggt ttcctggtaa tggctcagca ccagccagtc gtaggcgccc
     7321 caggccaggc cggccagcag caccagggcg gtgacgctca gcagcgccgg cttgcggcgg
     7381 gcgcggcggc gcccggcggc agctgggccg ggatcggcgc cttgcggcgc ggcgtgggca
     7441 ggggtgtcgt ggtcgtccat ggtgatgcgg tgcgaaatgc ggcaagcggc gccggcggcg
     7501 ccgggctcgg gttcaatggg gcgcgacgct gaccgtcgtg gtccccggcg agctgacggt
     7561 ggcgctgggc tgccagccgc cgcccatggc ctgcgtcagc ccgatctgcg cacccagcgc
     7621 ctgggccgcc aggtcgagct gctggcgccg ctgcgccagc acggcggttt cggcgccgag
     7681 cacctgcagg taagtgccca gaccggcctg gtaacgctgg cgggcaaggt cccaggcggc
     7741 ctcggcggcg gcctgggcct gcgcctgctc ggcctgctgg cgcgccagcg actgcacggc
     7801 gtgcgcctgg tcatgggcct cgcgcacggc gtcgatgacg gcggcgttgt agctttcgat
     7861 ggcggcgtcg acctcggcgc tgctggcgct cagatgggcg cgcagcttgc cggcctcgaa
     7921 gatcggcagg cgcagcgccg ggcccacgcc ccactgaaag ctgccgccct tgagcaagtt
     7981 gtcgaagccc aggctttgaa agcccagaaa gcccgccagg ttgatgttgg ggtagaactg
     8041 cgcgcgcgcc acctgcacct cgccgcgcgc ggcctccacg cgccagcgcg cggcgctgat
     8101 gtcggcgcgg cgcgccagcc agtcggcgtg cagttcggcg ggcacggcca gcgtcttgag
     8161 ctgcgccagg cgcggcgccg ccaccgcgtc ggccaggcgc ggctggccca gcagcgccgc
     8221 cagcgcctgg cgggcggcct gttgctgctg cgccagcgcg gccaagctgg tacgcgtgtc
     8281 ggcgcggctg ctttcgttct ggcgcagcga cacctggttg tccagcccgg cctgcgtgcg
     8341 ctgttgcacc agctgcgtca tctgctggcg ctgcgccagc gtgcgttcgg ccagctggtg
     8401 cagctcattg acgtgcgccc actgcacgta ggcacgaacc acgttggccg ccagcagcac
     8461 gcgcgcggcg tcggcctggg cctgggcggc gcgggtctgg ccgatggcgg cgtccagcgc
     8521 gccagcgtgc ttgccaaaga agtcgagctc ccagccccca ttgagctgca ggttggccag
     8581 cgtctgcacc gtgccgccca gcggcggcgg atagatgtag tggctggaaa aggactggcg
     8641 gttcaggtct gccgcaccgc ccacctgggg caggctggcg gcgcgcgcgg ccagctcgcc
     8701 ggcgcgcgcc ttggccactc gcgcggcagc cacgcgcagg ttcgggttgc cctccagcgc
     8761 ctgcagcacc aggctgtcga gctgtgcatc gcccagcgcg cgccaccagt cgggcgcggg
     8821 cgccaggtcc tggccggcgg gcaggccgag cgcggcggcg tcggtcagcc gcgcctgcgg
     8881 gcgaatgccc tgggtgtcgg cgcagccggc cagcgcgagc agcaccagcg ccgccgccag
     8941 gaggccacga gcagccggac ggggtgggcg ctgcgacgtc attggggggc ctttccctgt
     9001 tgcgccatgg cctcggcatt ggccaccagg cggtgcagca gcgcttgcag cagcgcccat
     9061 tcatccggcg aaaacccggc cagcgctcgg ttgtagacgg cgcacatgac ggccggcagg
     9121 cgctcggcga cggcctggcc ggcgggggtc aggcgcagtt gcaccacacg acggtcggcc
     9181 tgcgagcgct cgcgccgtac aaggtcgcgc tgctccaggc ggtcgagcat gcgcgtcatg
     9241 gtgctgacgt cgatgctgca ttgccgggcc agctcggcca cggtgtgggc gtcggcccgc
     9301 cacagcttga acagcggcag ccactgcggc aacgaggagt cctccatcgc cagctcgcgc
     9361 gtggtggctt gcgacaggcc gaccagcacc cgcttgagca ggtacatgat gctctgatcg
     9421 ggggtgaagg tttccggctg cagcagcggc gccgccgggg atgttgcggg gccggcagcg
     9481 ctgaaaggag gagcagttga ggcagtcatc gacttcgtcc gatgagggcc gcgccaggcg
     9541 ccggcctcag gaagaagccg ggaatttagt tgcaattgca aatattgtca acgcaatatt
     9601 tgccagggtg caggttggcc gtgtcagcgc cagccggcgt tcagccgctc gagcgcagcc
     9661 gcgcccgggc acaggtcggc cgtgtccagg ctgttgagtg gccgcgcgct ggtgtgctgg
     9721 gccgcgtgca ggtcacccgc ctcgccttcc aggtacagca ggccggtcac cagttcgccc
     9781 tgggccgcgt gggcctgcat gtaggccatg gcggccacgc ggtcggtggg gtcgtagtcg
     9841 gtgtgcagct tgcgcaggcg caggatggtg ccgtcgtgct gctccacgtc gaccacttcg
     9901 ccgggggcga tggtggcggt gatttcgtcg cgaccggtga tgaagtcgat gcggctgacg
     9961 gcctcgttgt gctcgcgcac ccagtcgtag ctgcgggtgg agccagcgtg gttgttgaag
    10021 gtgacgcagg ggctgatgac gtcgatgaag gcggcaccgc cgtggccgat ggcggccttg
    10081 atcagcggca ccagctgcgc cttgtcgccc gagaagctgc gcgccacgaa gctggcgccc
    10141 agctgcatgg ccagccccac caggtcgacc gggctgtcgg cgttgaccac gcccttcttg
    10201 ctctggctgc ccttgtcggc ggtggccgag aactggccct tggtgaggcc atagacgccg
    10261 ttgttctcga cgatgtagac catgttcacg ttgcgacgca tggcgtgggc gaactggccg
    10321 aggccaatgg aggccgagtc gccgtcgccc gacacgccca ggtacagcag gtcgcggttg
    10381 gccaggttgg cgccggtcag cacgctgggc atgcgcccat gcacggtatt gaagccatgg
    10441 ctggcgccga ggaagtagtc cggcgtcttg gacgagcagc cgatgcccga cagcttggcc
    10501 acgcggtgcg gctcgatgtc cagctcccag caggcctgca cgatggccgc cgacaccgag
    10561 tcgtgcccgc agccggcgca cagggtggag accttgccct cgtagtcgcg ccgggtgtag
    10621 ccgacctggt tcttggtgag cgacgggtgg tgcagccgcg gcttggcgat gtaggtcatg
    10681 acgggttctc ctgcggggcc acgaccgctt catgcacgtg gtcggtgatg gcctgggtga
    10741 tgaagcgggc ggtgatcggc gtgccgtcat agtgcagcac gcgcggcagc ttggcggggt
    10801 tgatctccag ctcgttgatc agcaggctgc gcatctgggc gtcgcggttc tgctcgacca
    10861 cgaacaccgt ttcgtgggcg tcgatgaatt ccttcacgct ggccgggaac gggaaggcgc
    10921 gcaggcgcag cgcgttgagg tggatgccct gccccgccag ctcgtccagc gcctcgtgca
    10981 tggcggggct ggtggagccg tagaagatca cccccagccg cgtgcgacgc gccgccgggc
    11041 gcggcaccgg ctgcggcacc agggtggcgg cggtggcgaa tttcttgagc aggcgctgca
    11101 cgttgtagat gtagtccggc ccgcgctcgg agtagcgtgc ataggcgtcg cgcgtggtgc
    11161 cgcgcgtgaa gtagctgccg cgcgtggggt gcgtgcccgg caatgtgcgc cacgggatgc
    11221 cgtcgccgtc gacgtccttg tagcggccga agtccttttg ctccaggtct tcggcgctca
    11281 tcaccttgcc gcggtcgtag cggcgcgcat cgtcccaggc cagcggctcg cacaggcgct
    11341 ggttcatgcc gatgtccaga tcggtcatca cgaacaccgg cgtctgcagg cggtcggcca
    11401 gatcaagcgc gcaggcggtg tgctcgaagc actcgcgcgg gtcttcgggc agcagcagca
    11461 cgtgcttggt gtcgccgtgc gaggcatagg cacaggccag gatgtcggac tgctgggtgc
    11521 gggtgggcat gccggtggac g
//
LOCUS       DAOOOG010000073        11511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6284_length_11511_cov_30.016323, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000073 DAOOOG010000000
VERSION     DAOOOG010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6284_length_11511_cov_30.016323"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PK242_07030"
     CDS             complement(<1..590)
                     /locus_tag="PK242_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOZ93806.1"
                     /translation="MSLFVTPQSMHFEAPLPLQSGASIGGYDLRYETYGQLNAERSNA
                     VLICHALNASHHVAGVYEGQERSEGWWDNMIGPGKPVDTDRFFVIGINNLGSCFGSTG
                     PMHVNPATGRAWGADFPVVTVEDWVNAQARLLDRLGIGSLAAVMGGSLGGMQALSWTL
                     QYPERVRHAVVVASAPNLSAENIAFNEVARRAIVTDP"
     gene            complement(722..973)
                     /locus_tag="PK242_07035"
     CDS             complement(722..973)
                     /locus_tag="PK242_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HOZ93807.1"
                     /translation="MAKRFPLRPAHPERICWGCDKYCPAGATACGNGSDRTPHPAELF
                     GDDWHTWQPPVEGGAAEMASPDVDDPSRDAPVAGGAGPR"
     gene            complement(995..1564)
                     /locus_tag="PK242_07040"
     CDS             complement(995..1564)
                     /locus_tag="PK242_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOZ93808.1"
                     /translation="METFLVATGIVALAEMGDKTQLLALLLAARYRRPWPIVWGIFIA
                     TVVNHALAGALGHAVTRVLGPELLRWVLGASFIAMAVWMLMPDRLDDDGTPAAAPRLG
                     VFGTTVLAFFLAEMGDKTQIATVALAARHPHWLAVVGGTTLGMMLANAPVVWFGDRLL
                     RRLPVRAVHGVSALIFLALGVLAWLGWDN"
     gene            1951..2505
                     /locus_tag="PK242_07045"
     CDS             1951..2505
                     /locus_tag="PK242_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOZ93809.1"
                     /translation="MGNKLYVGNLPYGVRDADLEQAFAPFGSVTSAKVMMERDTGRSK
                     GFGFVEMGSDAEAQAAINGMNGQSLGGRNVVVNEARPMEARPPRSGGYGGGGYGGGGG
                     GGYGGGRREGGGGYGGGRREGGGYGGGDSGGGYGGRREGGYGGGQDGNFRSPYGSGPR
                     GGRREGGYGGGRQDGGYGGGRDDE"
     gene            complement(2755..3546)
                     /locus_tag="PK242_07050"
     CDS             complement(2755..3546)
                     /locus_tag="PK242_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ93810.1"
                     /translation="MARLIFITGASSGIGQALAERCHAEGWHLALVARRADDLRAWAQ
                     ARGWSAERWRVYPADVADIDSIVAAGQACLAQQGVPDVVVANAGISVGMDTAERSDLA
                     VMARTLAVNNVGMAATFQPFVAAMRQRGSGTLVGIASVAAIRGLPGHGAYCASKAGVV
                     AYCESLRGELRGSGVRVVTLLPGYVDTPLTRKNRYPMPFLLSADRFAQRALRAIEQGV
                     SYRVIPWQMALVAKLLRVLPNAWFDRLLSGRPRKARADQGSATGS"
     gene            complement(3566..4183)
                     /gene="lptC"
                     /locus_tag="PK242_07055"
     CDS             complement(3566..4183)
                     /gene="lptC"
                     /locus_tag="PK242_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOZ93811.1"
                     /translation="MNRARLWRHGREQLAAWLPVLVMMLFALGSWWLVRSAPRIGPAD
                     GGPPPSAEPDYFMREFSVRSFDADGRLTSELVGVEGQHHPLTDTLAVQQPRIRSFDEQ
                     GNPTLASAQRALAKGDGSEAELFGKARVVREAVTSSDGRVVPRMEFLGEYLHAFIDEH
                     RVSSNQPVELRRGADVFTGNEFAYDDRRGVADLSGRVRGVLQPRR"
     gene            complement(4180..4752)
                     /locus_tag="PK242_07060"
     CDS             complement(4180..4752)
                     /locus_tag="PK242_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOZ93812.1"
                     /translation="MEPTPAAPALTWPPTLLLRAQAVRVAFFDVDGVLTDGGLYFSAE
                     GETLKRFHTLDGHGIKLLARAGITPCVISGRDSAPLRVRLQALGVTHVRLGTEDKLPA
                     ASDLLAELGLDWSQAAAIGDDWPDLPLLRRATLACAPPGAHAEARACAHHVTVARGGE
                     GAAREFCDLLLVAVGHYRRLLDAQHRGGAT"
     gene            complement(4752..5729)
                     /locus_tag="PK242_07065"
     CDS             complement(4752..5729)
                     /locus_tag="PK242_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOZ93813.1"
                     /translation="MHFDPAQALRLARETLDIEAAAVVGLKARLGDGFVRAVALMLAA
                     RGRVVVMGMGKSGHVGRKIAATLASTGTPAMFVHPAEASHGDLGMIQPQDVVLAISNS
                     GESDELVAILPLVKRLGAPLVAITGRADSTLARHADAVLDGSVEKEACPLNLAPTAST
                     TAQLALGDALAVALLDARGFRAEDFARSHPGGALGRRLLTRVADVMRAGDEVPRVGPE
                     ASFSALMREITHKGLGASAIVDADGRPVGIFTDGDLRRLVETGRDLRALTAREVMHPR
                     PRHIRDDALAAEAAEAMETHRITVLLVLDAGGRLVGAVNSNDLMRAKVI"
     gene            6152..8137
                     /locus_tag="PK242_07070"
     CDS             6152..8137
                     /locus_tag="PK242_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOZ93814.1"
                     /translation="MEPLELTLLYLAAAVLGVAACRWARLPAILGYLVVGVLIEPHTL
                     GWAVGQDAEGIKHLAEYGVVFLMFVIGLEFNLPKLHAMRQHVFGLGLAQVLGTMAVVT
                     GGALLLAWLLPQHWPMSWQGALALGGALAMSSTAIVVKMMSDRLELESEHGQRVIGVL
                     LLQDLAVVPLLVLIPALGSSPENLLAELTVAGLKAVGLITILLWGGQRVMRWWLTLVA
                     RRRSQELFVLNLLLMTLGLAWLTEQAGLSLGLGAFICGMLIAETEFKYQVEAEIRPFH
                     DVLLGLFFITIGMLLDWRLVLLQWPVVLILLALILLLKLAVVTGMAALLGASAGTALR
                     TGLYLAQAGEFGFVLLTLSNRQGLLAPELFNPILAAMVVSMLATPFIILGSNRIVLRL
                     VASEWMLQSLHVTRIARQSMDAAGHVLICGYGRSGQAMARLLRQQKIPFIALDIDPDR
                     VRLAAAAGDSVVFGDAATTAALVAAGLGRASTVALTYLDPPSVLKALAAIRAHAPKVP
                     VIVRTQTDRDLDKLRAAGATEVVPEGLEGALILAGHALALSGTPVRRVARLVQEQREQ
                     RYGMLRGFFPGATDDASADPMEEMRLGVFTVPASMAGQALGDMLVQLGELRAISLRLP
                     TGTPATPENEIVLQGGEVLLLSGRPEALARAEQALAT"
     gene            8158..8715
                     /locus_tag="PK242_07075"
     CDS             8158..8715
                     /locus_tag="PK242_07075"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOZ93815.1"
                     /translation="MSTAFFVSHYLRQHIRTVPDWPAPGVQFRDITPLLQNPQVFRVL
                     IDAFVHRYMDPALRPDVVAGLDARGFILGSVVAYELNVGFVPIRKKGKLPFTTVEETY
                     ELEYGSATVELHTDAVQRGQRVLLIDDLIATGGTMLAGWRLLQKLGAHVTEGAAIVEL
                     PELGGSARLTAAGLPLFTLVTFDGH"
     gene            8802..9452
                     /locus_tag="PK242_07080"
     CDS             8802..9452
                     /locus_tag="PK242_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ93816.1"
                     /translation="MNPPSVQDPAPHAPRRGRPRKSAQERDESQRRAELLRAAARLFR
                     HQGFDATSTRDIAAAAGMQSGSPFYYFESKSALLYAVMHDGMAMVTQAQAQALQALGP
                     RPTARERLRALIRHHFEVLVGPASDFIPVMLYEWRALTPEQRARIGALKDRYEAAWMP
                     VLGSLRRSGSLRARPEVARLFIFGALNWTVQWFDPRGPLSLDELTEQALRLFLGDR"
     gene            9453..10346
                     /locus_tag="PK242_07085"
     CDS             9453..10346
                     /locus_tag="PK242_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ93817.1"
                     /translation="MYQSVFAPRLLAGQTIVVTGGGSGIGRCSAHELAALGAHVVLVG
                     RNADKLRATADEITQDGGAVCWLACDIRQEGAVQETVRAVVAGRGRIDGLVNNAGGQY
                     MTPLEKISAKGWDAVVATNLTGGFLVARECFAQSMRQHGGAIVNIVADMWGSMPGMGH
                     SGAARAGMVSFTETAAVEWAAHGVRVNAVAPGYIASSGMDHYPPEAGAMLRAMRQTVP
                     LGRFGTEAEVSAAIAFLLSPAASFISGSVLRVDGARPQVRMGWPMGVPGASVRQRDAV
                     KPFNGFHRAVTPKVFAEPESP"
     gene            10343..>11511
                     /locus_tag="PK242_07090"
     CDS             10343..>11511
                     /locus_tag="PK242_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOZ93818.1"
                     /translation="MSLFESSWNAASDTAQARRARMLARIEAWRALEQRSADKSGEAA
                     ARFAKRGQLLPRERVALLLDRGAPWLPLAALAGYLRDVPEPDQSVPGGGMIAGIGFVA
                     GTRCMVVASDSGIDAGAVQPGGLDKMLRVQEIALRERLPFIHLVESAGADLMRYRVEG
                     FVLGGGLFRNLARLSAAGIPVITVQHGSGTAGGAYMPGLSDIVIMVEGRSRAFLAGPP
                     LLKAATGEVATEEELGGALMHTTVSGLGEYLARDDREALGLARRVIGDLQWSNRAPAL
                     AGQAPAAIKLEASEPPWPADELLTLMPAHHREPVDMREVAVRLVDGGALHEFKAGYGS
                     ATVCAQGHIGGHAVGFITNNGPIDNQGANKATHFIQWMCQLGHPIIYLQNTTGYMV"
BASE COUNT         1752 a         3961 c         4051 g         1747 t
ORIGIN      
        1 gggtcggtca cgatggcgcg ccgcgccacc tcgttgaagg cgatgttctc ggccgacagg
       61 ttgggcgcgc tggccaccac cacggcgtgg cgcacgcgct cggggtattg cagcgtccag
      121 ctgagcgcct gcatgccgcc caggctgccc cccatgacgg cggccagtga gccgatgccc
      181 aggcgatcga gcagccgcgc ctgcgcgttg acccagtctt ccaccgtcac caccggaaag
      241 tcggcgcccc aggcgcggcc cgtggcgggg ttgacgtgca tcggcccggt ggagccgaag
      301 cacgagccca ggttgttgat gccgatgacg aagaaacggt cggtgtcgac cggcttgccg
      361 gggccgatca tgttgtccca ccagccctcg ctgcgttcct gcccttcgta caccccggcc
      421 acatggtgcg aggcgttgag cgcgtggcag atcagcacgg cgttgctgcg ctcggcgttg
      481 agctggccgt aggtttcgta gcggaggtcg tagccgccga tcgaggcgcc gctctgcagt
      541 ggcagaggcg cctcgaaatg catggactgc ggcgtgacga acaaggacat ggcaacaaaa
      601 aacccggctt cgccaaatcg aaaccgggtt gcatgcgtgt ctcggattta gcggtatttg
      661 tagagcgccc gcaagcagag gcaaatcggc gctggatggg gccaaagtat agcggcaggc
      721 gtcagcgcgg gccagcgccg cccgcaacgg gcgcgtcgcg gctggggtcg tcgacgtcgg
      781 gcgacgccat ctcggccgcg ccaccttcga ccggcggctg ccatgtgtgc cagtcgtcgc
      841 cgaacagctc ggccgggtgg ggcgtgcggt cggagccgtt gccgcaggcc gtggcgccgg
      901 ccggacagta cttgtcacaa ccccagcaga tgcgctcggg gtgcgccggc cggagaggga
      961 acctcttcgc catggcggtg cggtgcaagc ggcctcaatt gtcccacccc agccatgcca
     1021 gcacacccag tgcaagaaag atcagcgccg acaccccatg cacggcgcgc accggcagcc
     1081 ggcgcagcag ccggtcgccg aaccacacca ccggcgcgtt ggccagcatc atgcccagag
     1141 tggtgccgcc caccacagcc agccagtgcg ggtgccgggc cgccagggcg acggtggcaa
     1201 tctgcgtctt gtcgcccatt tccgcgagga agaacgccag cacggtggta ccgaacaccc
     1261 ccaggcgcgg ggcggcggcc ggcgtgccgt catcgtccag ccgatcgggc atcagcatcc
     1321 acaccgccat ggcgatgaag gatgccccca gtacccagcg cagcagctcc ggccccagca
     1381 cgcgggtgac cgcatggccg agcgcgccgg ccagcgcatg attgaccacg gtggcgatga
     1441 aaatgcccca cacgatcggc cagggccggc gatagcgcgc cgccagcagc agggccagca
     1501 actgcgtctt gtctcccatt tcggccagcg cgacgattcc ggtggcaacg agaaaggttt
     1561 ccatggggaa gggcgtggcg aatacagaca aaatcgccat cgggcttgac aggtctgcgc
     1621 cagaagctat ttattcaata gcaaaaaacc acagtgcagc gacggcgcgc gaccggaaca
     1681 tggcggccga ttgtaggcgc gggcccaccg cgccgaccgt gaaccggccc ccgtcgcaaa
     1741 tacctgagtt tttttatcaa ccaaaaatcg cgcgcttgac tttcgcagtt ctgacgcata
     1801 atcgcgcggg tgtcccagcg gttggggcat tccacgtggc ttttgcggtg atttcacagt
     1861 ctcagttgcc cttcttggcc gtgccaaggt tcggtgaagg cgtttcgcgg actccatcgc
     1921 ttttcatttg tttttgagga gtccctttca atgggcaaca aactttacgt cggcaacctg
     1981 ccgtatggcg tgcgtgacgc cgatctggag caggccttcg cacctttcgg ctcggtcacc
     2041 agcgccaagg tgatgatgga gcgcgacacc ggccgctcca agggcttcgg gttcgtcgag
     2101 atgggcagtg acgccgaggc gcaggccgcc atcaacggca tgaacggcca gtcgctgggt
     2161 ggccgcaacg tggtcgtcaa tgaagcgcgc ccgatggaag cgcgtccgcc ccgttccggt
     2221 ggctacggcg gcggcggcta tggcggcggc ggtggcggtg ggtacggcgg cggccggcgt
     2281 gaaggcggcg gcggctacgg cggcggccgg cgtgaaggcg gtggctacgg cggcggcgac
     2341 tccggtggcg gctacggcgg ccgacgcgaa ggcggttacg gcggcggcca ggacggcaac
     2401 ttccgcagcc cctacggctc cggtccgcgc ggcgggcggc gtgagggcgg ttacggcggt
     2461 ggccgccagg acggcggcta tggcggcggc cgcgacgacg aataagcatc gtccccgcgc
     2521 tgctttcgcg ccatcgatac caaaggctcc gcaaggagcc tttttttcat cttggctgcg
     2581 gcccttgaac acacccgtcc gtaacgtggt cggtgtggcc gacacaacgc cacggtgcgg
     2641 cgcgcgcggg tgcagcgcgt gccgacccag caaacgaagg tcagcgacgc tgcgcgaccc
     2701 tggagggatt caaaccgccc gcagtcggcc agcggttcac ggccgttgca gggctcatga
     2761 ccccgtggcc gaaccctggt cggcgcgcgc cttgcggggc cggccgctca gcagacggtc
     2821 gaaccacgcg ttcggcaaca cgcgcaacag cttggcgacc agcgccatct gccacggaat
     2881 cacgcgatag ctcacgccct gttcgatggc acgcaacgcc cgctgtgcaa aacggtcggc
     2941 cgacagcaga aacggcatcg gatagcggtt cttgcgcgtc agcggcgtat cgacgtagcc
     3001 cggcagcagg gtgacgaccc tgacgccgct gccgcgcagc tcgccgcgca ggctttcgca
     3061 atacgccacc acgccggcct tgctggcgca gtacgcgcca tggccgggca ggccacggat
     3121 cgcggccacg ctggcgatgc ccaccagggt gccgctgccg cgctggcgca tcgccgccac
     3181 gaaaggctgg aacgtcgccg ccatgccgac gttgttgacc gccagcgtgc gcgccatgac
     3241 ggcgaggtcg ctgcgctcgg cggtatccat gccgacgctg atgccggcgt tggccaccac
     3301 cacgtcgggc acaccctgct gcgccagaca ggcctggccg gcggcgacga tgctgtcgat
     3361 gtcggccacg tcggccgggt agacccgcca gcgctcggcc gaccagccac gagcctgcgc
     3421 ccaggctcgc agatcgtcgg cacggcgcgc caccagggcc agatgccagc cttcggcatg
     3481 gcagcgttcg gccagcgcct gcccgatgcc gctggaggcg ccggtgatga agatcagcct
     3541 ggccatcgtg gtgcgggcga tcggctcagc gccgcggttg cagcacgccg cgcacccgac
     3601 cgctcagatc ggcgacaccg cggcgatcgt cgtaggcgaa ctcgttgccg gtgaacacgt
     3661 cggcaccgcg ccgcagttcg accggctggt tcgagctgac ccgatgctca tcgatgaagg
     3721 cgtgcaggta ttcacccagg aattccatgc gcggcaccac gcggccgtcg gatgacgtga
     3781 cggcctcgcg caccacgcgc gccttgccga acagttcggc ttcggagccg tcgcccttgg
     3841 ccagcgcgcg ctgggccgaa gccagcgtgg ggttgccctg ctcgtcgaac gaacggatgc
     3901 gcggctgctg caccgccagc gtgtcggtca gcggatggtg ctgaccttcg acgccgacga
     3961 gctcggatgt caggcgtcca tcggcatcga agctgcgcac ggaaaactca cgcatgaagt
     4021 agtccggctc ggccgacggt ggcggcccgc cgtccgccgg gccgatgcgc ggggcgctgc
     4081 gcaccagcca ccagctgccg agcgcgaaca gcatcatcac cagcaccggc agccaggccg
     4141 ccagttgttc gcgcccatgg cgccacagtc gcgccctgtt catgtcgcgc caccccggtg
     4201 ctgggcgtcc agcaggcggc ggtagtgccc cacggccacc agcagcaaat cgcagaactc
     4261 gcgcgcggca ccttcgccac cacgcgccac cgtcacgtgg tgcgcacagg cccgggcctc
     4321 ggcatgggcg cccggcggcg cgcaggccag ggtcgcgcgg cgcagcaacg gcaggtcggg
     4381 ccagtcgtcg ccgatggccg cggcctgcga ccagtccagc cccagctcgg ccagcaggtc
     4441 gctggccgcc ggcagcttgt cttcggtgcc caggcgcaca tgggtcacgc ccagcgcctg
     4501 cagacgcaca cgcagcggcg ccgagtcgcg ccccgaaatg acgcagggcg tgatgccggc
     4561 gcgggccagc agcttgatgc cgtggccgtc cagggtgtga aagcgcttga gcgtctcgcc
     4621 ctcggcgctg aagtacaggc caccatcggt cagcacaccg tcgacgtcga agaaggccac
     4681 gcgcactgcc tgcgcgcgca gcagcagggt cggcggccag gtcagtgcag gcgctgcggg
     4741 cgtgggctcc atcagatcac cttggcgcgc atcaggtcgt tgctgttgac cgcgccgacc
     4801 agccgcccac ccgcatccag caccagcagc accgtgatgc ggtgcgtttc catggcctcg
     4861 gcggcttcgg cggccagcgc atcgtcgcgg atgtggcgcg gccgcggatg catcacctcg
     4921 cgggcggtga gcgcgcgcag atcgcgcccg gtctcgacca ggcggcgcag gtcaccgtcg
     4981 gtgaagatgc ccaccggccg gccgtcggcg tcgacgatgg ccgaggcgcc cagccccttg
     5041 tgcgtgattt cgcgcatcag tgcgctgaaa ctggcctccg gaccgacccg cggcacctcg
     5101 tcgccagcgc gcatgacatc ggccacccgc gtgagcagcc gtcggcccag ggcgccgccc
     5161 gggtgcgagc gggcaaagtc ctcggcccgg aagccgcgcg cgtcgagcag ggccaccgcc
     5221 agtgcgtcgc ccagcgccag ctgcgccgtg gtgctggcgg tgggcgccag gttgagcggg
     5281 caggcttcct tttcgacgct gccgtcgagc acggcgtcgg catggcgcgc cagcgtggag
     5341 tcggcgcggc cggtgatcgc caccagcggc gcccccagcc gcttgaccag cggcaggatg
     5401 gcgaccagct cgtcgctttc gccactgttg gagatggcca gcaccacgtc ctgtggctgg
     5461 atcatgccca gatcgccatg gctggcctcg gccgggtgca cgaacatggc cggggtaccc
     5521 gtggacgcca gcgtggccgc gatcttgcga ccgacatggc cgctcttgcc catgcccatc
     5581 accaccaccc gcccacgcgc cgccagcatc aacgccacgg cacggacaaa gccgtcgccc
     5641 agccgcgcct tgaggccgac cacggccgcg gcctcgatgt ccagcgtctc acgggccaga
     5701 cgcagcgcct gggcagggtc gaagtgcatc gcaggattct agtgtggtgc gctgccatgg
     5761 cttgtgcttt cagagcccca gaaggcgtcc agacaaggcg cagcccgaag cccatggctg
     5821 atcccttggc cagggctgca acgcggcatg ggcgccttcc gggggctccc tttgggcgag
     5881 ccgctgagcg actgcctgtg gcgttgcgcc cgcttgcatc gagccgctca gcgactcgct
     5941 gaaagtgcca gtccactgaa acattgaaat gggcggcgag ggacacccga gacgggaggc
     6001 gatgcaaggc gcaaagcgca gccacagccc gtgctgtggc gagcattggc aacgccgcag
     6061 cgtgcccgca tcgggcggac ggcgccgatc cgatttcagt gtttcagtgg acccgccatg
     6121 gcggcgcacc acaccacggt agcatgcggg catggagccc ctggagctga cgctgctgta
     6181 cctggccgcc gccgtgctgg gcgtggcggc ctgccgctgg gcgcgcctgc cggccatcct
     6241 gggctacctg gtggtgggtg tgctgatcga gccgcacacg ctgggctggg cggtcggtca
     6301 ggatgccgag ggcatcaagc acctggccga gtacggcgtg gtgttcctga tgttcgtgat
     6361 cgggctggag ttcaacctgc ccaagctgca cgccatgcgc cagcacgtgt tcggcctggg
     6421 tctggcgcag gtgctgggaa ccatggccgt ggtgaccggc ggcgccctgt tgctggcctg
     6481 gctgctgccg cagcactggc cgatgagctg gcagggtgcg ctggcgctgg gcggcgcgct
     6541 ggccatgagc agcaccgcca tcgtggtcaa gatgatgagc gaccggctgg agctggagtc
     6601 cgagcacggc cagcgcgtca tcggcgtgct gctgctgcag gacctggcgg tggtgccgct
     6661 gctggtgctg atccctgcgc tcggttcgag cccggagaac ctgctggccg agctgaccgt
     6721 ggccggactg aaggcggtgg gcctgatcac catcttgctg tggggtggac agcgggtgat
     6781 gcgctggtgg ctgaccctgg tggcgcgccg ccgcagccag gaactgttcg tgctcaacct
     6841 gctgctgatg accctggggc tggcctggct gaccgaacag gcgggccttt cgctggggct
     6901 gggcgccttc atctgcggca tgctgatcgc agagaccgag ttcaagtacc aggtggaggc
     6961 cgaaatccgt cccttccacg acgtgctgct gggcctgttc ttcatcacca tcggcatgtt
     7021 gctggactgg cgcctggtgc tgctgcaatg gccggtggtg ctcattctgc tggcgctgat
     7081 cctgctgctg aagctggccg tggtgacggg catggccgcc ctgctgggcg ccagcgccgg
     7141 cacggcgctg cgcaccggcc tgtacctggc gcaggcgggc gagttcggtt tcgtgctgct
     7201 gacgctcagc aaccgtcagg gactgctggc gccggagctg ttcaacccga tcctggccgc
     7261 catggtggtg tcgatgctgg ccacgccgtt catcatcctg ggcagcaatc gcatcgtgct
     7321 gcgtctggtg gccagcgaat ggatgctgca gtcgctgcac gtgacccgca ttgcccgcca
     7381 gtcgatggac gccgccggcc atgtgctgat ctgcggctat ggccgcagcg gccaggccat
     7441 ggcgcgcctg ctgcggcagc agaagattcc gttcatcgcg ctcgacatcg accccgaccg
     7501 ggtgcgcctg gcggccgccg ccggcgacag cgtggtgttc ggtgacgccg ccaccacggc
     7561 ggcgctggtt gctgccggtc tggggcgtgc cagcacggtg gccctgacct atctggatcc
     7621 gcccagcgtg ctcaaggcgc tggcggcgat ccgcgcgcat gcgcccaagg taccggtgat
     7681 cgtgcgcacc cagaccgacc gcgacctcga caaactgcgc gccgccggcg ccaccgaggt
     7741 ggtgcccgaa ggcctggagg gcgcgctgat cctggccggc catgcgctgg cgctgtcggg
     7801 cacgccggtg cggcgtgtgg cgcgcctggt gcaggagcag cgcgaacagc gctacggcat
     7861 gttgcgcggc ttctttcccg gcgccaccga cgacgccagc gccgacccga tggaagagat
     7921 gcgcctgggc gtgttcacgg taccggcgtc gatggccgga caggccctgg gagacatgct
     7981 ggtgcagctg ggcgagttgc gcgccatcag tctgcgactg cccacgggca cgccggcgac
     8041 gcccgaaaac gagatcgtcc tgcaaggcgg cgaggtcctt ctgctgtccg gccgccccga
     8101 ggcgctggca cgggcggagc aggccctggc cacctgatgg cgcccgataa tggcgccatg
     8161 agcaccgcct ttttcgtctc gcactacctg cgccagcaca tccgcaccgt gcccgactgg
     8221 ccggcgccgg gcgtgcagtt tcgcgacatc acgccactgc tgcagaaccc gcaggtgttt
     8281 cgcgtgctca tcgacgcctt cgtgcaccgc tacatggacc ctgcgctgcg ccccgacgtc
     8341 gtcgccggcc tggatgcgcg cggcttcatt ctgggcagcg tggtggccta cgagctcaac
     8401 gtcggcttcg tgccgattcg caagaagggc aaactgccct tcaccacggt cgaggaaacc
     8461 tacgagctcg aatacggcag cgccacggtg gagctgcaca ccgatgccgt gcaacgcggc
     8521 cagcgcgtgc tgctgatcga cgacctgatc gccaccggcg gcaccatgct ggccggctgg
     8581 cgcctgctgc agaagctcgg cgcccacgtg accgagggcg ccgccatcgt cgagctgccg
     8641 gaactgggag ggtcggctcg gctgaccgcc gccgggttgc ccttgttcac gctggtgacc
     8701 ttcgacgggc actgaaggcg ccctggccac ccttggccac gcaccttatt tttaccaagc
     8761 aatcgcttga ttaaataaaa gcgctctgct acgcttgagg gatgaaccct cccagcgtcc
     8821 aggacccagc gccgcacgct ccgcgccgcg ggcgcccgcg caagagcgcg caggagcgcg
     8881 acgaaagcca gcgccgtgcc gagcttctgc gcgcggcggc gcggctgttt cgccatcagg
     8941 gtttcgacgc caccagcaca cgcgacatcg ccgctgctgc gggcatgcag agcggctcgc
     9001 cgttctacta cttcgaaagc aagagcgcgt tgctgtacgc cgtcatgcac gacggcatgg
     9061 ccatggtcac ccaggcgcag gcacaggcgc tgcaggcgct ggggccgcgg cccacggcgc
     9121 gcgagcggct gcgggcgctg atccgccacc acttcgaggt gctggtgggg ccggccagcg
     9181 acttcatccc ggtcatgctg tacgagtggc gcgcgctcac gcccgaacag cgcgcgcgca
     9241 tcggcgcact gaaagaccgc tacgaagccg cctggatgcc ggtgctgggc agcctgcgcc
     9301 gcagcggatc gttgcgggcg cggccggagg tggcgcggct gttcatcttc ggcgcgctca
     9361 actggacggt gcagtggttc gatccgcgcg gcccgctgtc gctcgatgaa ctgaccgaac
     9421 aggcgctgcg ccttttcctt ggagatcgct agatgtacca atccgtgttt gcgccccgcc
     9481 tgctggcggg gcagaccatc gtcgtcaccg gcggtggctc gggcatcggc cgctgcagcg
     9541 cgcatgagct ggcagcgctg ggcgcccacg tggtgctggt ggggcgcaac gccgacaagc
     9601 tgcgcgccac cgccgacgag atcacccagg acggcggcgc cgtgtgctgg ctggcctgcg
     9661 acatccgtca ggaaggcgcc gtgcaggaaa cggtgcgcgc cgtggtggcc ggacgcgggc
     9721 gcatcgacgg cctggtcaac aacgccggcg gccaatacat gacgccgctg gaaaaaatca
     9781 gcgccaaggg ctgggacgcg gtggtggcca ccaacctgac cggcggcttt ctggtggcgc
     9841 gcgagtgttt tgcgcagtcg atgcggcagc acggcggcgc catcgtcaac atcgtggccg
     9901 acatgtgggg ctcgatgccg ggcatgggcc acagcggtgc ggcgcgtgcc ggcatggtca
     9961 gcttcaccga aaccgcggcg gtcgagtggg cggcgcacgg cgtgcgcgtc aacgcggtgg
    10021 cgccgggcta catcgcctcc agcggcatgg accactaccc gcccgaggcc ggtgccatgc
    10081 tgcgcgccat gcgccagacg gtgccgctgg ggcgcttcgg caccgaggcc gaggtgtcgg
    10141 cggcgatcgc ctttctgctc agcccggcgg ccagcttcat cagcggcagc gtgttgcgcg
    10201 tggatggcgc gcgccctcag gtgcgcatgg gctggcccat gggcgtgcca ggcgcatcgg
    10261 tgcgccagcg cgacgccgtc aaacccttca acggctttca cagggcggtc acgcccaagg
    10321 tgtttgccga gccggagtca ccatgagtct gtttgaatcg agctggaacg ccgccagcga
    10381 caccgcccag gcgcgccgcg cccgcatgct ggcgcgcatc gaagcctggc gtgcgctgga
    10441 gcagcgcagc gccgacaagt ccggcgaggc cgccgcccgt tttgccaagc gcggccagct
    10501 gctgccacgc gagcgcgtgg ccctgctgct cgaccgcggc gcgccctggc tgccgctggc
    10561 cgcgctggcc ggttatctgc gcgacgtgcc cgagcccgat caatcggtgc ccggtggcgg
    10621 catgattgcc ggcatcggct tcgtggctgg cacgcgctgc atggtggtgg ccagcgactc
    10681 gggcatcgac gccggggcgg tgcagcccgg cgggctggac aagatgctgc gcgtgcagga
    10741 aatcgcgctg cgcgagcgcc tgcccttcat ccacctggtc gagagcgccg gcgccgatct
    10801 gatgcgctac cgggtcgagg gcttcgtgct gggtggcggg ctgtttcgca acctggcgcg
    10861 cctgtcggcc gctggcattc cggtcatcac tgtccagcac ggctcgggca cggcgggcgg
    10921 cgcctacatg ccggggctgt cggacatcgt gatcatggtc gaggggcgct cgcgcgcctt
    10981 cctcgccggc ccgccgctgc tcaaggccgc caccggcgag gtcgccaccg aggaggagct
    11041 gggcggcgcg ctgatgcaca ccacggtctc tggcctgggc gaatacctgg cgcgcgacga
    11101 ccgcgaggcg ctcggcctgg cgcgccgggt gatcggcgat cttcaatggt caaatcgtgc
    11161 tccagcgctt gctggacaag cgccagcagc tattaaatta gaagcatccg aaccaccctg
    11221 gccggccgac gagctgctca ctctgatgcc ggcccaccac cgcgagccgg tggacatgcg
    11281 cgaggtcgcg gtgcggctgg tcgatggcgg cgcgctgcac gagttcaagg ccggctatgg
    11341 cagcgccacc gtgtgcgcgc aggggcacat cggcgggcat gcggtgggct tcatcaccaa
    11401 caacggcccg atcgacaacc agggggccaa caaggccacg cacttcatcc agtggatgtg
    11461 ccagctgggc caccccatca tctacctgca gaacaccacc ggctacatgg t
//
LOCUS       DAOOOG010000074        11380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6377_length_11380_cov_22.520000, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000074 DAOOOG010000000
VERSION     DAOOOG010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11380
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6377_length_11380_cov_22.520000"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK242_07095"
     CDS             <1..189
                     /locus_tag="PK242_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93819.1"
                     /translation="PPADIPALARMLSRLSVFAHRAGDTLESIDLNPVLALPAGQGAF
                     ALDALVQTRNPSHEVSCP"
     gene            186..983
                     /locus_tag="PK242_07100"
     CDS             186..983
                     /locus_tag="PK242_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ93820.1"
                     /translation="MSKRYDFQGCNVFVSGGTSGINLGIAEAYAAAGARVAVMSRNAE
                     RVADAQARLAGHGQPALGLVGDVRDPARVQQVLAEVHAAWGAIDVLVSGAAGNFLARA
                     LDLSPNGFKTVIDIDLMGTFHVLRAAHPFLRQPGASIINISAPQSYNPAPLQVHACAA
                     KAGVDQVMRVLAMEWGSHGIRVNSIVPGPIEGTTGLAKLASDDARARMAERVPLGRLG
                     RLNDIAAMALFLGSEDASYITGALIPVDGGISLLGARNHAIESGAQA"
     gene            980..1762
                     /locus_tag="PK242_07105"
     CDS             980..1762
                     /locus_tag="PK242_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOZ93821.1"
                     /translation="MTDSILHAEAFDGVLQLQLRAPEVRNALSAEMKRALHEQARRFV
                     EDDGLRCLLITGCDAVFCAGGDLRTMQSDRSTVGVRRRMALTHASVRLLAECEKPIVT
                     AVNGAAVGAGLSLALMGDVVVAAEDAYFMAGFANVGVLPDMGVLYHLPRTVGRVQAND
                     LLLSNRRVNAQEALELGLVSRALPADGFLAQAQALARSIAAGPAVSFGLARALMAGAY
                     KDSFGEFLLKESLAQAVVFGTDDFAEGVSAFGDKRKPRFTGR"
     gene            1796..2773
                     /locus_tag="PK242_07110"
     CDS             1796..2773
                     /locus_tag="PK242_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ93822.1"
                     /translation="MQALIKVLCSAACAGALLLAGAAHANYPDRPVKLIVPYAPGGIT
                     DNLARALAEGVSRELKQPIVVENKAGASAMIGTGMVANAPADGYTLLMASNGNMTVTP
                     LVMKKLTYDPIKELRVIAIVGELPTVAVTNLQTPATDLRSFAAYAKANDGKLNFASLG
                     QGNVLYLTARKIEGELGIRMTEVPYKGSAPSLTALMANDVQFYIDVLPGALPFIQSGK
                     LKALAVPMSKRISWLPDVPTLEEAGYGAFHAASWLGWAVPAATPPDVVARLQGAIGRF
                     AQDEKFRATFTKAGVLMLPAMTPAQVDEYLKADRERWGALIRQHNITID"
     gene            2780..3940
                     /locus_tag="PK242_07115"
     CDS             2780..3940
                     /locus_tag="PK242_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ93823.1"
                     /translation="MHAHDLDADRALREEVRAFLREALPDTLRAKVLGHAPLTKDDYV
                     SWQRILNARGWLAPAWPVARGGTGWTPRQIYIFEEECWLAGAPEVFAFGPKMLAPVLM
                     QHGTPAQQDEFLPRILSSEDWWCQGYSEPGAGSDLASLRTRGRIEGDTLVIDGQKTWT
                     TYAQYANRMFCLVRTDPQAKSQEGISFVLLDMDMPGITVRPLILLDGGHEVNEVFLDQ
                     VRVPLSRVVGPLNQGWTVAKALLGHERFNVGKIGRSLRELRLLKRLAAQPDALGRSLL
                     QDVRFAERIAAVEVDLLALEQTTLRLIAEVQAGRSVGAEASTLKIRGTEIAQQLSLLL
                     VQACGDCTAPMGDGLPTPMALVRQYLNWRKLSIYGGSNEVQHNIIAKFALGI"
     gene            3957..5111
                     /locus_tag="PK242_07120"
     CDS             3957..5111
                     /locus_tag="PK242_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ93824.1"
                     /translation="MSFHQEHDALRESANRFLIEQCGFERHRQRQQSADAGSNLALWR
                     QMAELGWLGITLGEPWGGMAQSPRAALSLIEAMGAQFVPAPYAEALAATHLLQLLGRP
                     DQQATWLPRLCEGAALGLVAHVEPGARYELDCVSTRADKVEQGFRLSGRKSAVPWGDA
                     ADLLLVSARTGDAPGQRGDLSLFVVERSTAGLRFEGHAGIADERWADVILDHVVVPQA
                     ALLGPAGEAQDALDGTHAFLVAADCAEAVGTMDAVLQETREYASTRKQFGTPLARFQV
                     IAHRLVDMFIQVQLARSMAQLAASIVDDGPNTSAARLRLAACKAQISHACRFVGEQAV
                     QIHGGIGMTDELALSHRVRRLLAIDRRRGDQFHHLGTLAESVQQGVGLYA"
     gene            5133..6386
                     /locus_tag="PK242_07125"
     CDS             5133..6386
                     /locus_tag="PK242_07125"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOZ93825.1"
                     /translation="MNSARPRADHQAAQPPLHGIRVIDMSSVLMGPFASQALGDMGAD
                     VIKIEAPAGDVVRQIGPARHADMGPIFLNTNRNKRSVVLDLKHADGVAALKRLLGDAD
                     VLLYNVRPQAMTRLGLDYDSVRALNPRLVYAGVFGFGQDGPYAARPAYDDLIQGAATL
                     PYLMARASGDIPRYVPTAVADRIVGQVAVGAILASLLARQRTGLGDRVDIPMFETMVG
                     FVLGDHLGGLTYDPPLDGGGYARQLSPERRPYRTRDGYICALVYNDKQWAGFLRGIGR
                     STLPQEDPRFASFASRAAHIDHVYGWLAQVFEQRSTQEWFDLLQEADVPCMPMHDLHS
                     VLSDPHLLATGFFQTMEHPSEGTVRSMRLAPRWQTAQIATPRPAPRLGEHTRQVLQES
                     GFSTPEIDRLLHVRAAIAAAPEESA"
     gene            6383..7522
                     /locus_tag="PK242_07130"
     CDS             6383..7522
                     /locus_tag="PK242_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOZ93826.1"
                     /translation="MNRAGPLQGVRVVEFVGIGPAPLCGMLLADLGAEVLRVERPTPS
                     GLGIERPAQFDLMRRGKRTLRLDLKAAAGLDLARALVRRADALIEGFRPGVMERIGLG
                     PEVCLQDNPALVYGRVTGFGQHGPLSQTAGHDLNYIALTGALDAIGRQGQPPTPPLNL
                     LGDYAGGSLFLALGLLSALLHARSSGEGQVVDAAMIDGVGALMAPFHGLQAAGLHAGP
                     RGTNVLDSGAPYYDVYACADGEFISIAAIEPKFRAELRERLRVAGAAVDDLPDFDDRG
                     RWPELRTRLVALFASQTSAYWCAQLEGSDACFAPVLSVGAAAQHPHHIARASFMVVDG
                     VTQPAPAPRFSATPCGVPSGPTQGGDAKEWALAWGVPAELIAHMD"
     gene            7531..7986
                     /locus_tag="PK242_07135"
     CDS             7531..7986
                     /locus_tag="PK242_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOZ93827.1"
                     /translation="MAGLYFEEFHVGQQFVHPWSRTVTEMDNTLFSLLTMNPQPLHLD
                     AHFAAQTEWGQRLFNSLYTLGILVGMSVNDTTLGTTVANLGMTDVRFPKPVFHGDTLR
                     ARTEVISVRASQSRPESGLVEFEHTGLNQRDEVVAICRRTALMRRRPSA"
     gene            8051..8611
                     /locus_tag="PK242_07140"
     CDS             8051..8611
                     /locus_tag="PK242_07140"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOZ93828.1"
                     /translation="MAPAWYRHLSRAVDVIDNVLVSLGCGLLFALMCLVVADVARRYL
                     FNAPIAWSYEVINHYVMPGVFFYTVAHTLKAHAHVSVDILHNYVQRRTRYVFELIGSV
                     LALPVFVLATVLAAGKTLEDFETAAQASSGLAVPTWTIGMVVPIGFGMLSLRLALNVI
                     GYVSTLATGRAIKALPPISGTEEGAE"
     gene            8608..9897
                     /locus_tag="PK242_07145"
     CDS             8608..9897
                     /locus_tag="PK242_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOZ93829.1"
                     /translation="MTVALIMLALFILLAIGMPVAFALAFAGTLGLYLVGGWQALSGV
                     LATAPLSSVESYELISVPLFILMAEFVILSGVADRLFAAAATWVGRVPGGLAMATALA
                     GAGFGAISGSSTASAATLSSTTLPAMLKQGYEARLSGGVVAISGTLAMLIPPSIALIL
                     YGIIADVSIGALLIGGVIPGILVTLVIIATVWVLVLINPAAAPKGRAYTVREKLASLR
                     VVGPMLFLFMAVTGVIYTGIATPTEASGLGAFGAFLIVLWERRLTRAGLSKALVHAAH
                     ASCMIVMIIVCAKIFGYFFTLTQSTQALVAWVGALDVSRWVILALILLGYLVLGCLMD
                     QVAILILTVPIVLPVIKALGFDPVWFGVIVILMAEVGLVTPPVGLNAFVVARYAKRPL
                     AEIFGGVWPHVISHLLLVAVLVAFPQIILWLPSKMGG"
     gene            9926..10945
                     /gene="dctP"
                     /locus_tag="PK242_07150"
     CDS             9926..10945
                     /gene="dctP"
                     /locus_tag="PK242_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOZ93830.1"
                     /translation="MIKLLPITLAVAASLCAAGHAAAQTKIKVADSFPVGHYLPRYFT
                     TPMMERLKADPAAKGIEFEYYPAEQLGKAKDLLSLTQSGLVDVAYVGPGFVSDKMPLS
                     VVAELPLPYTGSCQATLAYWNLAKPGGLLDKKEFAPNGVRLLFTIVLPPYQVITRKPF
                     ASLKDLEGMKIRASGSAKELLLKELKAVPVLMPTPEVYESLSRGTIDGVLFPFNSLAP
                     YGIDKLSKSGTIGSNFGSYVANWVISEKRYQSLPPALQKELAAMGDELTRSVCKQVER
                     DEAGDVDKTRAAGVQLKPLSPADQQALEQVAAQVATDWAQKLDRRGKSGTEVLNAFKA
                     GLQAK"
     gene            11031..>11380
                     /locus_tag="PK242_07155"
     CDS             11031..>11380
                     /locus_tag="PK242_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOZ93831.1"
                     /translation="MTPFLLYQQDGPVVTLTLNAPDTRNVLTGNSAPDEFSEACARIN
                     GDASVRAVILTGAGAAFSAGGNLKDMQQMFDWSPDRLRNWYRTGVQRLALAVYHLEVP
                     VICAVNGPAIGAGC"
BASE COUNT         1796 a         3703 c         3839 g         2042 t
ORIGIN      
        1 cccccagccg acataccggc gctggcccgc atgctgtcgc gcctgtcggt gttcgcccac
       61 cgcgccggcg acacgctgga gagcattgat ctcaacccgg tgctggcatt gcccgcgggg
      121 cagggcgcct tcgcgctgga cgccctggta cagacccgca atccgtcaca cgaagtgagc
      181 tgcccatgag caaacgctat gactttcagg gctgcaacgt gttcgtctcc ggcggcacca
      241 gcggcatcaa cctgggcatt gccgaggcct atgccgccgc cggcgcacgc gttgcggtga
      301 tgagccgcaa cgccgaacgc gtggccgacg cccaggccag gctggcaggc cacggccagc
      361 ccgccctggg gctggttggc gatgtgcggg atccggcacg ggtgcagcag gtattggccg
      421 aagtgcatgc cgcctggggc gccatcgatg tgctggtgtc gggtgcggcc ggcaattttc
      481 tggcccgcgc gctcgatctg tcgcccaacg gcttcaagac cgtgatcgac atcgacctga
      541 tgggcacctt tcacgtgctg cgcgcggctc acccgttcct gcgtcaaccg ggcgcgtcga
      601 tcatcaacat ctcggcgccg cagtcctaca acccggcgcc cctgcaggtc catgcctgcg
      661 ccgccaaggc cggggtggat caggtcatgc gcgtgctggc gatggagtgg ggcagccacg
      721 gtatccgtgt caactccatc gtgccggggc cgatcgaggg cacgacgggg ctggccaagc
      781 tggcctcgga cgacgcccgc gcccgcatgg cggagcgtgt gccgctgggt cgcctgggcc
      841 ggctgaacga catcgcggcc atggcgctgt ttctgggctc cgaggacgcg agctatatca
      901 ccggcgcgtt gatcccggtc gatggcggca tttccttgct gggcgcgcgc aatcatgcca
      961 tcgagtccgg agcacaggca tgaccgacag catcttgcac gctgaggcct tcgatggtgt
     1021 cctgcagttg cagctgcgcg cgcccgaagt gcgcaacgcc ctgagcgccg agatgaagcg
     1081 ggcgctgcat gagcaggcgc gccgcttcgt cgaagacgac ggcctgcgat gcctgctgat
     1141 cacagggtgc gacgcggtgt tctgcgccgg cggcgatctg cgcaccatgc aaagcgatcg
     1201 cagcaccgtc ggcgtgcgcc gacgcatggc gctcacgcac gccagcgtgc gtctgctggc
     1261 cgaatgcgag aaacccatcg tcaccgccgt caatggcgcc gcggtgggcg ccggcctgtc
     1321 cctggccctg atgggggacg tggtggtggc ggccgaagac gcgtatttca tggccggctt
     1381 tgccaacgtg ggggtgctgc cggacatggg cgtgctctac cacctgccgc gtaccgtggg
     1441 ccgcgtgcag gccaatgacc tgctgctcag taatcgccgg gtcaacgccc aggaggcgct
     1501 ggagcttggc ctggtcagcc gcgcgctgcc agcggatgga tttctggcgc aggcgcaggc
     1561 cctggcccgt tccatcgccg ctggcccggc cgtctcgttt ggactggcgc gcgccctgat
     1621 ggccggcgcc tacaaggaca gctttggcga gttcttgctc aaggaaagcc tggcgcaggc
     1681 cgtggtgttt ggcaccgacg actttgccga gggcgtcagc gcgtttggcg acaagcgcaa
     1741 accccgtttc accggccgct gagtgcggcc ttcatcacct caaccaggag acaccatgca
     1801 agccttgatc aaagtcctgt gctcggccgc ctgtgccggc gcgctgttgc tggccggcgc
     1861 cgcacatgcc aactaccccg accgccccgt caagctgatc gtgccctatg cgcccggtgg
     1921 catcaccgac aacctggcgc gtgccctggc cgaaggtgtg agccgcgagc tcaagcaacc
     1981 catcgtcgtt gaaaacaaag cgggcgccag cgccatgatc ggcaccggca tggtggccaa
     2041 cgcgccggct gacgggtaca cgctgctgat ggcgtccaac ggcaacatga cggtgacgcc
     2101 gctggtgatg aaaaaactca cctatgaccc gatcaaggaa ctgcgcgtga tcgccatcgt
     2161 gggcgagttg ccgaccgtgg cggtgaccaa cctgcagacg ccagccaccg acctgcgctc
     2221 gtttgccgcc tatgcaaaag ccaacgacgg caaactgaac tttgcctctc tcgggcaggg
     2281 taatgtgctg tatctgacgg cccgcaagat cgagggcgag ttgggcatcc gcatgaccga
     2341 agtgccctac aagggcagcg caccgtcgct gacggcgctg atggccaacg atgtgcagtt
     2401 ctacatcgac gtgctgccag gggcgctgcc gttcattcag agcggcaagc tcaaggccct
     2461 ggcggtaccg atgagcaagc gcatttcgtg gctgcctgac gtgccgacgc tggaagaagc
     2521 aggctatggc gccttccacg ccgccagctg gttgggctgg gccgtgccgg ctgccacccc
     2581 gcccgacgtg gtggcccgcc tgcagggcgc cattggccgc ttcgcacagg acgaaaaatt
     2641 ccgcgccacc ttcaccaagg ccggtgtgct gatgctgccg gccatgaccc ccgcccaggt
     2701 cgacgaatac ctgaaggccg accgcgagcg ctggggcgcg ctgatccgcc agcacaacat
     2761 caccatcgac tgacgcgcca tgcatgccca cgatctggac gcggaccgcg ccctgcgcga
     2821 agaagtgcgc gcctttctgc gcgaggccct gcccgacacc ctgcgagcca aagtgctggg
     2881 ccatgccccc ctgaccaagg acgactacgt cagctggcag cgcattctga atgcgcgcgg
     2941 ctggctggcg ccagcctggc cggtggcgcg cggtggcacg ggatggacgc cgcgccagat
     3001 ctacattttt gaagaggagt gctggctggc aggcgcgccc gaggtgttcg ccttcgggcc
     3061 gaagatgctg gcgccggtgt tgatgcagca cggcacgccg gcgcagcagg acgagttttt
     3121 gccacgcatt ctgagcagcg aggactggtg gtgtcagggg tattcggagc cgggcgccgg
     3181 atcggacctg gccagcctgc gcacgcgtgg tcgcatcgaa ggcgacaccc tggtgatcga
     3241 tggtcagaag acctggacca cctacgcgca atacgccaac cgcatgttct gcctggtgcg
     3301 caccgacccc caggccaagt cccaggaagg catttccttc gtgttgctgg acatggacat
     3361 gccgggcatc acggttcgac cgttgatcct gctcgatggg ggtcacgagg tcaatgaagt
     3421 gttcctcgac caggtccgcg tgcccctgag ccgggtggtt ggtcccttga accagggctg
     3481 gacggtggcc aaggcgctgc tcgggcatga gcgcttcaat gttggcaaga tcggccgctc
     3541 gctgcgcgag ctgcgcctgc tcaagcgcct ggcggcgcag cccgatgcgc tggggcgctc
     3601 gctgctgcag gatgtccgct ttgccgagcg cattgccgcc gtcgaagtcg atctgctggc
     3661 gcttgagcag accaccctgc gcctgatcgc cgaagtacag gcgggtcgga gcgtcggcgc
     3721 cgaggcgtca acgctgaaaa ttcgcggcac cgagatcgcc cagcaactgt cgctgctgct
     3781 ggtgcaggcg tgtggcgatt gcacggcgcc gatgggcgat ggcctgccca ctccgatggc
     3841 gctggtgcgc caatacctga actggcgcaa attgtcgatc tacggcggct ccaacgaggt
     3901 tcagcacaac atcattgcca agtttgcgct tggcatctga cgagcggatg caatcgatgt
     3961 ctttccacca agagcacgac gcgctgcgcg agagcgcaaa ccgatttctg atcgaacagt
     4021 gcggctttga acggcatagg cagcggcaac aatcggccga cgccgggtcc aatctcgcct
     4081 tgtggcgaca aatggctgag ctgggttggc tcgggatcac gctgggcgag ccatggggcg
     4141 gcatggccca gtcgccccgc gccgcgctgt cgctgatcga ggcgatgggc gcccagttcg
     4201 ttcccgcgcc ttacgccgaa gccctggccg ccacacatct gttgcaactg ctgggacggc
     4261 ccgatcagca ggcgacatgg ctgccccgcc tgtgcgaagg cgccgccttg ggcctggtcg
     4321 cccacgtcga gcccggagcc cgatacgagc tggactgcgt gagcacgcgg gccgacaagg
     4381 tggagcaggg atttcggttg agcggccgca agagtgccgt gccctggggt gacgccgccg
     4441 atctgctgct ggtgtcagcc cgtaccggag acgcgccagg acaacgcggt gacctgtctc
     4501 tcttcgtggt cgaacgaagc accgcgggcc tgcgcttcga gggccacgcc ggaatcgcgg
     4561 atgaacgatg ggccgatgtg attctggacc acgtcgtggt gccgcaggcc gcgctgctgg
     4621 gccccgccgg ggaggcgcag gatgcgctcg atggcaccca tgcgttcctg gtggcagccg
     4681 attgcgccga agccgtgggc accatggatg ccgtgctgca ggaaacgcgc gagtacgcca
     4741 gcacgcgaaa gcaatttggc acgccgctgg cgcgctttca ggtgattgcc catcgcctgg
     4801 tggacatgtt cattcaggtg cagttggcgc gctccatggc gcagctggct gcgtccatcg
     4861 tggacgatgg cccgaacacc tccgcggcac gcctgcgatt ggcagcctgc aaggcgcaga
     4921 tcagccatgc gtgccgtttc gttggcgagc aggcggtgca gatccacggc ggtatcggca
     4981 tgacggacga actggcgctg tcgcaccggg tgcgtcgcct gctggccatc gatcggcggc
     5041 gcggtgatca gtttcatcac cttggcacgc tggccgagag cgttcagcaa ggcgtggggc
     5101 tttatgcctg agacaggatg gatcaacgca tcatgaattc ggcccgcccc cgcgcagatc
     5161 accaggccgc ccaacccccg ctgcatggca tccgggtcat cgacatgagc tcggtgctga
     5221 tggggccgtt tgcctcgcag gcgctgggcg acatgggtgc ggatgtcatc aagatcgagg
     5281 cgccggccgg tgacgtggtg cgccagatcg gcccggcccg tcacgcggac atgggcccga
     5341 tctttctcaa caccaaccgc aacaagcgca gcgtggtgct ggacctcaaa catgccgatg
     5401 gcgtggcggc cctcaagcga ttgctgggcg atgccgacgt gctgctctac aacgtgcggc
     5461 cccaggccat gactcggctg ggactggact acgacagcgt gcgggcgctc aatccccggt
     5521 tggtctacgc cggggtgttc ggcttcggtc aggacggccc ctatgcggcg cgccctgcct
     5581 atgacgacct gatccagggc gcggccacgc tgccctacct gatggcacgc gccagcggcg
     5641 acattccgcg ctacgtcccc actgccgtgg ccgaccgtat cgtcggccag gtggccgttg
     5701 gcgccatcct ggccagcctg ctggcgcgcc agcgcaccgg cctgggcgac cgcgttgaca
     5761 tccccatgtt cgaaaccatg gtcggcttcg tgctgggcga ccatctgggg ggcctgacat
     5821 atgacccgcc gctggatggt ggcggctatg ccaggcagct ctcccccgaa cgccggccgt
     5881 accgcacgcg cgatggctac atctgcgccc tggtctataa cgacaagcag tgggcgggat
     5941 ttctgcgcgg catcggccgc agcacgctgc cgcaggaaga cccccgcttt gccagctttg
     6001 ccagccgcgc cgcgcacatc gaccatgttt atggctggct ggcgcaggtg ttcgagcagc
     6061 gcagcacgca ggagtggttt gacttgctgc aggaggccga cgtgccctgc atgccgatgc
     6121 acgatctgca cagcgtgctg agcgatccgc acctgctggc caccgggttc tttcagacca
     6181 tggagcatcc cagcgaagga acggtgcgct cgatgcgcct ggcgccgcgc tggcaaaccg
     6241 cccagattgc gacgccgcgc ccggcgccgc gcctgggaga gcacacccga caggtactgc
     6301 aggagtcggg cttcagcacc ccggaaatcg accgcctgct gcatgtgagg gcggccattg
     6361 cggctgcgcc ggaggagtcc gcatgaaccg ggcagggccg ctgcaaggtg tgcgcgtggt
     6421 cgaattcgtc ggcattggcc cggcgccgct gtgcggcatg ctgctggccg atctgggggc
     6481 cgaggtgttg cgggtggagc ggccaacgcc cagcggcctg gggatcgagc ggccggcgca
     6541 gttcgatctg atgcggcgtg gcaagcgaac cctccggctc gatctgaagg ctgccgccgg
     6601 tctggacctg gcgcgcgcgc tggtgcgcag ggccgatgcg ctgatcgagg gttttcgccc
     6661 cggtgtcatg gaacgcatcg gattggggcc ggaggtttgc ctgcaggaca acccggcgtt
     6721 ggtgtacggc cgcgtcaccg gatttggcca gcacgggccg ctgtcgcaga cggccgggca
     6781 cgatctgaac tacatcgccc tgaccggcgc gctggacgct atcggccggc aagggcagcc
     6841 gcccacgccg cccctgaacc tgctggggga ctacgccggt ggttccctgt ttctggctct
     6901 ggggctgctg tccgctctgt tgcacgcccg cagcagcggc gaggggcagg tggtggatgc
     6961 cgcgatgatc gacggtgtgg gtgcgctgat ggcgccgttc cacggtctgc aggcggctgg
     7021 gctgcatgcc ggaccgcgcg ggaccaacgt gctcgattcc ggcgcccctt actacgacgt
     7081 ctatgcctgc gccgatggcg agttcatctc gatcgccgct atcgagccga agttccgcgc
     7141 cgagttgcgc gagcgcctgc gcgtggccgg tgctgctgtc gacgatttgc cggatttcga
     7201 cgatcgaggg cgctggcccg agctgcgcac ccgattggtc gccctgtttg ccagccagac
     7261 gagcgcgtac tggtgtgcgc agctggaagg gtccgacgcc tgctttgccc cggtgctttc
     7321 ggtgggcgcc gcggcgcagc acccccatca catcgcgcgc gccagcttca tggtggtcga
     7381 tggcgtgacg caacccgcgc cggcgccgcg gttcagcgcc accccctgcg gtgtgcccag
     7441 cggaccgaca cagggcggcg acgccaagga atgggcgctg gcctggggcg tgccggcgga
     7501 actgatcgca cacatggact gaggagcatc atggcaggtc tctacttcga ggaatttcac
     7561 gtcggtcagc agtttgttca cccctggtcg cgcaccgtga ccgagatgga caacacgctg
     7621 ttttccctgc tgaccatgaa tccgcagccg ctgcatctgg acgcccactt cgcggctcag
     7681 accgaatggg ggcaacgcct gttcaacagc ctgtacacgc tgggcatcct ggtgggcatg
     7741 agcgtcaacg acaccacgct gggcaccacg gtggccaacc tcggcatgac ggacgtgcgc
     7801 tttcccaagc ccgtgttcca tggcgacacg ctgcgcgccc gcaccgaggt gatctcggtg
     7861 cgcgccagcc agtcccggcc cgaatcgggc ctggtcgagt tcgagcacac cggcctgaat
     7921 cagcgggacg aagtcgtcgc gatctgtcgg cgtacggccc tgatgcgcag aaggccgtct
     7981 gcatagagcg tccgttgtgt ctctcgattc gaaaggctga ccgatgaatc aaggtgatcc
     8041 tgaagcaccc gtggcgcccg cctggtaccg gcatctgagt cgagcggtcg acgtgatcga
     8101 caacgtgctg gtgagcctgg gctgtggact gctgtttgcc ctgatgtgcc tggtggtggc
     8161 ggatgtggcg cgccgctacc tgttcaacgc gcccattgcg tggtcatacg aggtcatcaa
     8221 ccactacgtc atgccggggg tgttcttcta caccgtggcc cacacgctga aagcacacgc
     8281 ccacgtgtcg gtggacatcc tgcacaacta tgtgcagcgt cgtacccgct atgtgttcga
     8341 gttgatcgga tcggtattgg cactgccggt gttcgtgctg gccaccgtgc tggccgccgg
     8401 caagacgctg gaggattttg aaactgcggc ccaggccagc agcggcctgg ccgtgcccac
     8461 atggaccatc ggcatggtcg tgcccatcgg cttcggcatg ctgagcctgc ggttggccct
     8521 gaacgtgatc ggctacgtca gcaccttggc cactggccgc gccatcaaag cgctgccacc
     8581 catctcgggc accgaggagg gggccgaatg accgttgcac tgatcatgct ggcgctgttc
     8641 atcctgctgg ccatcggcat gccggtggct tttgcgcttg cctttgccgg cacgctgggc
     8701 ttgtatctgg tgggtggctg gcaggctttg tcgggcgtgc tggcaaccgc tccgctgtcg
     8761 agcgtcgaat cctacgaatt gatatcggtg ccgctgttca tcctgatggc cgaattcgtc
     8821 attctgagtg gtgtggcaga caggctgttt gccgcggccg ctacctgggt cggccgcgtg
     8881 ccgggcggct tggccatggc cacggcgctg gccggcgccg gcttcggtgc gatttcaggg
     8941 tccagcacgg cttccgccgc gacgctgtcg tccaccacct tgcctgccat gctcaagcag
     9001 ggttacgagg ctcgtctgag tggtggtgtg gtggccatct cgggcacctt ggccatgctg
     9061 atcccgccat cgatcgccct gatcctgtac ggcatcattg ccgacgtcag cattggcgcg
     9121 ctgctgatcg gcggcgtgat tcccggcatc ctggtgacgc tggtgatcat cgccaccgtg
     9181 tgggtgctgg tgctgatcaa ccctgcggcg gcgcccaagg ggcgagccta cacggtgcgc
     9241 gagaagctgg cctcgctgcg cgtcgtgggg ccgatgctgt tcctgttcat ggccgtgacc
     9301 ggcgtcatct acaccggcat cgccacgccc accgaggctt cggggctggg agcgttcggt
     9361 gccttcctga tcgtgctgtg ggagcgccga ctcacccgcg cgggtctatc caaggccctg
     9421 gtgcacgcag cccatgccag ctgcatgatc gtgatgatca tcgtctgcgc caagatcttt
     9481 ggctacttct tcacgctcac acagagcacg caggctctgg tggcgtgggt gggggcgctg
     9541 gatgtgtccc gctgggtcat cctggcgctg attctgctgg gctatctggt cctgggctgc
     9601 ctgatggatc aggtggcgat cctgattctg acggtgccga tcgtgctgcc ggtgatcaag
     9661 gcgctgggct ttgacccggt gtggttcggc gtgatcgtga tcctgatggc cgaggtcggt
     9721 ctggtcacgc cgcccgtggg cctgaacgcc tttgtggttg cccgctacgc caagcggccg
     9781 ctggccgaga ttttcggtgg cgtctggcca cacgtgatca gtcacctgct gttggtggcc
     9841 gtgctggtgg cgtttccgca aatcatcctg tggctgccct ccaagatggg cggatgaatt
     9901 ttcaacgcga taaaggagac atgacatgat caagctgctt cccatcaccc tggccgttgc
     9961 cgccagtctg tgtgccgccg gccacgcagc cgcgcagacc aagatcaagg tcgcggattc
    10021 attccccgtc gggcactacc tgccgcggta cttcaccacc cccatgatgg agcgcctgaa
    10081 ggccgatccg gcagccaagg gcatcgagtt cgagtactac ccggccgagc agttgggcaa
    10141 ggccaaggat ctgctctcgc ttacccagtc cgggctggtc gatgtcgcct atgtgggccc
    10201 cggctttgtg tcggacaaga tgccgctttc ggtggtggcc gagttgccgc tgccctacac
    10261 cggctcctgc caggcgacgc tggcctactg gaatctggcc aaaccgggcg gcctgctgga
    10321 caagaaggaa tttgcgccca acggcgtgcg cctgttgttc accatcgtgc tgccgcccta
    10381 tcaggtgatc acgcgcaagc cgtttgccag ccttaaggat ctggaaggga tgaagatccg
    10441 cgcgtcgggg tcggccaagg agctgctgct caaggagctc aaggcggtgc cggtgctgat
    10501 gcccacgcct gaagtctacg aatcgctctc gcgcggcacc attgacgggg tgctgtttcc
    10561 gttcaacagc ctggccccct acggcatcga caagctctcg aagtcgggca cgatcggctc
    10621 caactttggc tcctacgtgg ccaactgggt catcagcgaa aagcgctacc agtcgttgcc
    10681 accagcgctg caaaaggagc tggcagccat gggtgacgag ttgacgcgca gcgtctgcaa
    10741 acaggtcgag cgcgatgaag cgggcgacgt cgacaagact cgcgcggcag gcgtgcagct
    10801 caaaccgttg tcccctgcgg accagcaggc gctggagcag gtggctgccc aggtggccac
    10861 ggattgggcg caaaagctcg accgccgcgg caagagcggt accgaggtgc tgaatgcgtt
    10921 caaggccggg ttgcaggcca agtgacgggt gacgcgcttc tccgatttcg ccagcaccgg
    10981 cctcgtgagc agccggtgct gacctcgacc tttgcagcaa ctcgccttcc atgacgccct
    11041 ttcttctcta tcagcaggac ggccccgtcg tcaccctcac gctcaatgcg cctgatacgc
    11101 gcaacgttct gaccggcaac agcgcgccgg acgagttttc tgaagcctgc gcgcgcatca
    11161 atggcgatgc ctcggtgcgt gccgtcatcc tgaccggggc gggcgcggcg ttttccgccg
    11221 gcggaaactt gaaggacatg cagcagatgt tcgactggtc gcccgatcgg ctgcgcaact
    11281 ggtaccgcac cggagtgcag cgactggcgc tggcggtcta ccacctggag gtgccggtga
    11341 tctgcgccgt caacggcccg gccatcggcg ccggctgcga
//
LOCUS       DAOOOG010000075        11329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6424_length_11329_cov_25.225475, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000075 DAOOOG010000000
VERSION     DAOOOG010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6424_length_11329_cov_25.225475"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PK242_07160"
     CDS             <1..719
                     /locus_tag="PK242_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ93832.1"
                     /translation="WFFLTADYAFGHALERDTSEVVKASGGKVLGSVKVPLGTADFSS
                     FLLQAQSSKAKIVGLANAGGDTINSIKQAAEFGIVKGGQKLAGLLVFLPDVHGLGLNT
                     AQGLNITEAFYWDLNDGTRAWSKRFAAAHGGKHPSSDHAGVYAGVLHYLKAVDAAKTD
                     DGSKVVAKMKELPTDDPLFGKGTVRADGRKIHPVYLFEVKKPSESKAPYDYYKLVQTI
                     AADKAFRPLNEGNCPLVAKK"
     gene            749..1633
                     /locus_tag="PK242_07165"
     CDS             749..1633
                     /locus_tag="PK242_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ93833.1"
                     /translation="MEIFGIPIQAMMGQLLIGLINGSFYALLSLGLAVIFGLLNIINF
                     AHGAQYMLGAFGAYLLLNKLGLGYWWSLLVVPILVGATGVVIERTMLKPLYKLDHLYG
                     LLLTFGLALIIQGLFRNEYGSTGLPYAIPEALQGGKNLGFMFLPNYRAWVIVASLVTC
                     LSTWFVIEKTRLGGYLRAATENPQLVQAFGINVPRMITLTYGFGVALAALAGVMAAPI
                     YQVSPQMGENLIIVVFAVVVIGGMGSIMGAIVTGFGLGLIEGLTKVFYPEASTTVIFV
                     IMTIVLLIRPAGLFGAQK"
     gene            1740..2633
                     /locus_tag="PK242_07170"
     CDS             1740..2633
                     /locus_tag="PK242_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ93834.1"
                     /translation="MALIFIGAPLVGVYPVFMMKVMCFALFACAFNLLLGYGGLLSFG
                     HAMFLGSAGYVSAHAAKVWGLTPELAIALGASCAGALGWVAGRLAIRRQGIYFAMITL
                     ALAQLVFFFSLQAPFTGGEDGIQAVPRGKLLGLIDLANPAAMYPFVLAIFLGGFLLIY
                     RIVHSPFGQVLKAIRENEARAISLGYDADRFKHRAFVLSATLAGLAGATKAIVFQLAS
                     LTDVHWSMSGEVVLMTLVGGMGTLFGPVVGAAVIVSMQNYLAQLGAWVTVVQGAIFVL
                     CVLLFRRGIIGEIGHLLKKSL"
     gene            complement(2735..4312)
                     /locus_tag="PK242_07175"
     CDS             complement(2735..4312)
                     /locus_tag="PK242_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOZ93835.1"
                     /translation="MPAGSGFKLTYSTMFNPPPELHARFDAALASFRRDGMGRDHAQW
                     IGGMSVGGARHFEVRSPIDQDWLIGRFVEGSAQDVDRAVQAAQAAYPGWSATPWRERV
                     ALLRRAARLIEERVYAISAAVALEVGKNRMESIGEVQETADLIDWYCDQMEAHDGFDR
                     ALPDDPLPQFRSHNRTVLRPYGVWAVIAPFNFPFALAGGPLGPALVAGNTAVFKVSPE
                     TSLSGRLLLDALHDAGLPAGVINFVTGGDATGRALVEHPGVAGITFTGSQAVGMQVLR
                     QSVQGPFPRPCIVEMGGKNATIVSRRADLERAALGIVRSAFGLSGQKCSACSRVYVEQ
                     PVADDLRARLVRLAGELSVGDPTQQAHWMGPVIGRAAYERYAGLAEHLRQVGRIDTGG
                     RWLTAGALARGFFVAPTVAQAPLDDRAWVDEHFLPLVLVGEVASVAEGISRANASDYG
                     LTAGFYGTPDEVAYFHRHIEAGVTYANRPQGATTGAWPGYQPFGGWKGSGSTGKAIGS
                     FYYLPQYLREQSRTVVE"
     gene            complement(4412..5599)
                     /locus_tag="PK242_07180"
     CDS             complement(4412..5599)
                     /locus_tag="PK242_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ93836.1"
                     /translation="MTADERRASLSLAGIFALRMLGLFLVLPVFALEAARYPGGDDPA
                     LVGLAMGIYGLTQAVMQVPLGLASDRIGRKKVIVAGLLVFALGSALAALAGSVHMLVA
                     GRALQGAGAVSAAVTALLADLTRDEVRTKAMALVGISIAAMFALSLMAAPPLTGAIGL
                     SGLFWLTAALALAGVVLVLVATPAEPVQHVDAPRGRLQEVWRHPHLWRLDLGVFVLHA
                     VQMAMWVVVPGLLVQAGLLKAEHWKLYLPAVLVSFVFMGGLFALERRGHLRAVLRGGI
                     ALLLLVQLALLLLASRAPGLWTLGVVMFFFFMAFNLIEASQPGLISRLAPAQARGAAL
                     GVYNTLQSLGLFAGGALGGWLLKGWGPAAVLSGTSALVALWLLLGWGQHVPALRPPAD
                     PPR"
     gene            5732..6235
                     /locus_tag="PK242_07185"
     CDS             5732..6235
                     /locus_tag="PK242_07185"
                     /inference="COORDINATES: protein motif:HMM:NF015907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ecotin family protein"
                     /protein_id="HOZ93837.1"
                     /translation="MKATHHLPRATAVLALACTLLAACAQQPAAPNPSAAQELKAFPA
                     TLKGHQRHVIELPALPDEADRQIELIGTKAMTVDCNTRGMDGRFESRDVQGWGYNYWV
                     LQSRGQVMGTMMACPPGSQRPGVAQTQGTLLRYNSKLPVVVFVPEGMGLRWRVWSAGA
                     MQDASAR"
     gene            6524..7447
                     /locus_tag="PK242_07190"
     CDS             6524..7447
                     /locus_tag="PK242_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93838.1"
                     /translation="MSFLQSTSNTAFFIALGIVVLLSGIGFGIVAWVGRLTGALRGRE
                     PVPINMLTEGYLLARGKASGRTLRAPLSGRPCVWWEMKVWERRIEIDRQASSDEPDRR
                     ASWTLLRHDVSRRPIHCTQGFVTCTVQPDGMTLTVPSEVRDWKGKQYPPENRDPPVQP
                     GSAFSYRQVHDTGFTIVGGKLLGDRYRYVELLIASNAELFVLGRVERVAARAGAASTH
                     DDAPAPEGESVAPFEQAQREENEDEQMSPSMREVQWRIAPEKGRPYIVATQPPEQWMA
                     TIKLASQGGLIVGTVTAALAAFMLWARYQGG"
     gene            complement(7511..8023)
                     /locus_tag="PK242_07195"
     CDS             complement(7511..8023)
                     /locus_tag="PK242_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93839.1"
                     /translation="MRSMHCGSLRSATRRAAIGLALVTLVAAGAQAQQASGELALQAR
                     SGVVKVAPRYAYLLAGPSFDGKPMRRLVLSEVDLTSAIQKCDRLGCVSSDLHSGVTVD
                     FDGGPRLGYWFVGKGQMVQHSDTAQPATMQLKQDSHQRLAGSWTLKGDVGATGSVTFD
                     APLAKTFARN"
     gene            8287..11319
                     /gene="uvrA"
                     /locus_tag="PK242_07200"
     CDS             8287..11319
                     /gene="uvrA"
                     /locus_tag="PK242_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOZ93840.1"
                     /translation="MNDLAAPDLSDAEGLPLATALRQPRISVRGARTHNLKNIDIDIP
                     KDRLVVITGLSGSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQLMDKPDVDLIEGLS
                     PAIAIEQKATSHNPRSTVGTVTEIHDYLRLLFARAGTPHCPEHDLPLTVQTVSQMVDA
                     TLALPQDTRVMVLAPLARARKGEFSELLAQMQALGYVRFRIDGQVVEADRLPVLQKND
                     KHDIDVVIDRLKVRGTADGPGGAATALAARLADSFEAALRLADGRAIALEMDEGKEHL
                     FNSKFACPVCGHSLPELEPRLFSFNAPAGACPTCDGLGTTDVFDPERVVAFPSLSLAS
                     GAIKGWDRRNGYYFALIESLAKHYRFSVETPFEELPEAVRHVILHGSGDEALRFSYTF
                     DSGAQAGRKVSKKHPFEGILPNMARRWRETDSNVVREELARYRSQQPCTACGGTRLRQ
                     EARHVFVGEGAQRRAIYQISHVTLGEAQAYFDALRLTGAKADIAERVVREIASRLKFL
                     NDVGLGYLSLERSADTLSGGESQRIRLASQIGSGLSGVMYVLDEPSIGLHQRDNDRLI
                     ATLQHLRDLGNTVLVVEHDEDMMRAADHIVDLGPGAGVHGGRVMAQGTFAQVAATPAS
                     VTGQFLRGERAIAVPSRRRRSHIDSCSRTPDGRQSPETLETHRPTPSGVLRIVGARGH
                     NLKDVTVDVPVGLLTCVTGVSGSGKSTLVNDTLYTAAAREIHRAHAEPAPHEAIEGLA
                     QFDKVIAVDQSPIGRTPRSNPATYTGLFTPIRELMAETAMARERGWGPGRFSFNVPQA
                     SGGGRCEACQGDGVVKVEMHFLPDVYVPCDVCAGQRYNRETLQVQWKGRNIAQVLDMT
                     VEAAHAFFADQPAIARKLQTLLDVGLSYITLGQSATTLSGGEAQRVKLALELSKRDTG
                     RTLYVLDEPTTGLHFADVELLLKVLHQLVEAGNTVVVIEHNLDVIKTADWLIDMGPEG
                     GDGGGTVVAQGTPEQVAGHPDSHTGRYLKPVLVAAAAA"
BASE COUNT         1881 a         4010 c         3743 g         1695 t
ORIGIN      
        1 gctggttctt cctgaccgcc gactacgcct tcggccacgc cctggagcgc gacacctccg
       61 aagtggtcaa ggcctcgggc ggcaaggtgc tgggcagtgt caaggtaccg ctgggcacgg
      121 ccgacttctc ctccttcctg ctgcaggcac agtcgtccaa ggccaagatc gtcggcctgg
      181 ccaacgccgg cggcgacacc atcaactcga tcaagcaggc ggccgagttc ggcatcgtca
      241 agggcggcca gaaactcgcc ggtctgctgg tgttcctgcc cgacgtgcac ggcctgggtc
      301 tgaacaccgc ccagggcctg aacatcaccg aggccttcta ctgggacctg aacgacggca
      361 cgcgcgcctg gagcaagcgc tttgctgccg cgcacggcgg caagcacccg tcgtccgacc
      421 acgccggcgt ctatgccggc gtgctgcact acctgaaggc ggtggacgcc gccaagaccg
      481 acgacggcag caaggtggtg gccaagatga aggagctgcc caccgacgat ccgctgttcg
      541 gcaagggcac ggtgcgcgcc gatggccgca agatccaccc ggtgtacctg ttcgaggtca
      601 agaaacccag cgagtccaag gccccgtacg actactacaa gctggtgcag acgatcgcgg
      661 ccgacaaggc cttccgaccg ctgaacgagg gcaactgccc gctggtggcc aagaagtaag
      721 tgcgagcagc cgcaaggcat agacaaccat ggaaatcttc ggtatcccga tccaggccat
      781 gatgggccag ttgctcatcg gcctcatcaa tggctcgttc tatgccttgc tctcgctggg
      841 cctggccgtg atcttcggcc tgctcaacat catcaacttt gcccacggcg cgcagtacat
      901 gctgggcgcc ttcggcgcct acctgctgct caacaagctg gggctgggct actggtggtc
      961 gttgctggtg gtccccatcc tggtgggcgc caccggtgtc gtcatcgagc gcaccatgct
     1021 gaaaccgctg tacaagctgg accacctgta cggcctgctg ctgaccttcg gcctggccct
     1081 gatcatccag ggcctgttcc gcaacgagta cggctccacc ggcctgccct acgccattcc
     1141 cgaggcgctg caggggggca agaatctggg cttcatgttc ctgcccaatt accgcgcctg
     1201 ggtgatcgtg gcctcgctgg tgacctgcct gagcacctgg ttcgtgatcg aaaaaacccg
     1261 cctgggtggc tatctgcgtg ccgccaccga aaacccccag ctggtgcagg ccttcggtat
     1321 caacgtgccg cgcatgatca ccctgaccta cggcttcggc gtggccctgg ccgcgctggc
     1381 cggcgtgatg gcagcgccca tctaccaggt cagcccgcaa atgggcgaaa acctgatcat
     1441 cgtcgtgttc gcggtggtgg tgatcggcgg catgggctcc atcatgggcg ccatcgtcac
     1501 cggcttcggg ctgggcctga tcgagggcct gaccaaggtg ttctatcccg aagcctccac
     1561 caccgtcatc ttcgtcatca tgaccatcgt gctgctgatc cgcccggcgg gtctgtttgg
     1621 cgcgcagaaa taaagccatg gccagcgccc acaaccccac cgcgcctgcc gccgcgccgg
     1681 cagcccaccc ccggaacgcc agcgatccgg cacccaccca cagggtggcc ttcatcctca
     1741 tggccctcat cttcatcggt gcgccgctgg tcggcgtgta cccggtgttc atgatgaagg
     1801 tgatgtgttt tgccctgttt gcctgcgcct tcaacctgct gctgggttac ggcggcctgc
     1861 tgtcgttcgg gcacgccatg tttctgggca gcgccggcta cgtgtcggcg catgcggcca
     1921 aggtctgggg cctgacgccg gagctggcca tcgccctggg tgcgtcgtgc gccggcgcgc
     1981 tgggctgggt cgccggcagg ctggcgatcc gccgtcaggg catctacttc gccatgatca
     2041 ccctggctct ggcgcagctg gtgttcttct tcagtctgca ggcgcccttc accggtggcg
     2101 aagacggcat ccaggcggtg ccgcgcggca agctgttggg cctgatcgac ctggccaacc
     2161 ccgccgccat gtaccccttc gtgctggcga tcttcctggg cggcttcctg ctgatctacc
     2221 gcatcgtgca ttcgcccttc ggtcaggtgc tgaaggccat ccgcgaaaac gaagcgcgcg
     2281 ccatttcgct gggctacgac gccgacaggt tcaagcaccg cgccttcgtg ctgtcggcca
     2341 cgctggccgg gctggcaggt gccaccaaag ccatcgtgtt ccagctggcc tcactcaccg
     2401 acgtgcactg gagcatgtcg ggcgaagtgg tgctgatgac cctggtgggc ggcatgggca
     2461 ccttgttcgg cccggtggtc ggggcggccg tcatcgtctc gatgcagaac tacctggccc
     2521 agctgggggc ctgggtgacg gtggttcagg gcgccatctt cgtgctgtgc gtactgctgt
     2581 tccgccgcgg catcatcgga gaaatcgggc acctgctgaa gaaatcgctc tgagcctgcc
     2641 ctttgcgtca ccccaagcgc ccgtcacggg cgcttttttg ttcgatggag ggcgctgaga
     2701 cccggctcgg tcccgacccg atccgggtgg cggctcactc gaccaccgtg cgcgactgtt
     2761 cgcgcagata ctgcggcagg tagtagaacg agccgatggc tttgccggtg ctgccgctgc
     2821 ccttccagcc gccaaacggc tggtagcccg gccatgcacc ggtggtggcg ccctgcgggc
     2881 ggttggcgta ggtgacgcca gcttcgatgt gccgatggaa ataggccacc tcgtcgggcg
     2941 tgccatagaa gcccgcggtc aggccatagt cgctggcgtt ggcgcgggag atgccttcgg
     3001 cgaccgatgc cacttcgccc accagcacca gcggcagaaa atgctcgtcc acccaggcgc
     3061 gatcgtccag tggcgcctgc gccaccgtgg gagcgacgaa aaatccacgc gccagcgcgc
     3121 ccgcggtcag ccagcgcccg ccggtgtcga tgcggccgac ctgccgcaga tgctcggcca
     3181 ggcccgcgta gcgctcgtaa gcggcgcggc cgatgaccgg ccccatccag tgcgcctgct
     3241 gcgtcggatc cccgaccgac agctcgcccg ccagccgcac caggcgggca cgcagatcgt
     3301 ccgccacggg ctgctccaca tagacgcgcg agcaggccga acatttctgt cccgacagac
     3361 cgaacgccga acgcacgatg cccagcgccg cccgctccag gtcggcgcgg cggctcacga
     3421 tggtggcgtt cttgccgccc atctcgacga tgcagggtcg ggggaagggc ccctgcaccg
     3481 actggcgcaa cacctgcatg ccgaccgcct gcgagccggt gaaggtgatg cccgccacgc
     3541 ccgggtgctc gaccagcgcg cgtccggtgg cgtctcctcc ggtgacgaag ttgatgaccc
     3601 cggccggcag accggcgtcg tgcagggcgt ccagcagcag ccgcccggac agcgaggttt
     3661 ccggcgacac cttgaacacc gccgtgttgc ccgccaccag cgccggtccg agcggcccgc
     3721 ccgccagtgc aaacggaaaa ttgaacggcg cgatcaccgc ccacacgccg taggggcgca
     3781 gcacggtgcg gttgtggctg cgaaactgcg gcagcgggtc gtctggcagc gcgcggtcaa
     3841 agccgtcgtg ggcttccatc tgatcgcaat accagtcgat caggtcggcc gtctcctgca
     3901 cctcgccgat cgactccatg cggttcttgc ccacctccag cgccacggcg gcgctgatgg
     3961 catagacccg ctcttcgatc aggcgggcgg cacggcgcag cagcgccacg cgctcgcgcc
     4021 acggcgtggc cgaccagccc ggataggcgg cctgcgcggc ctgcaccgcg cggtcgacgt
     4081 cctgggcgct gccctccacg aagcgcccga tcagccagtc ctggtcaatc ggtgaacgca
     4141 cctcgaaatg ccgcgcccca cccaccgaca tgccaccgat ccactgtgcg tggtcgcgcc
     4201 ccatgccgtc gcgccgaaag ctggccagcg ccgcgtcgaa gcgcgcatgc agctccggcg
     4261 gcggattgaa catggtcgaa taggtcagct tgaagccgga accagcgggc atgtgaacct
     4321 ccagaagact gcattcaacc aaaaaacgac cgtggggcgc gcccacccgg cagacgaggg
     4381 tcagccaagc cgagccaacc ggtgttcgcg ttcaccgcgg cggatccgcc gggggacgca
     4441 gcgccggcac atgctggccc cagcccagca gcaaccacaa cgccaccagc gccgacgtgc
     4501 ccgacagcac cgccgccggt ccccagccct tgagcagcca gccgcccagc gccccgccgg
     4561 caaacaggcc cagcgattgc agggtgttgt acacgcccag ggcggcgccg cgcgcctggg
     4621 ccggcgccag gcgcgagatc aggccgggct ggctggcttc gatcaggttg aaggccatga
     4681 agaaaaagaa catcaccacg cccagcgtcc acagccccgg cgcgcgcgag gccagcagca
     4741 gcagagccag ctgcaccagc agcagcaggg caatgccgcc gcgcagcacg gcgcgcaggt
     4801 ggccgcgccg ctccagcgcg aacagaccgc ccatgaacac gaacgagacc agcacggccg
     4861 gcaggtacag cttccagtgc tcggccttca gcagcccggc ctgcaccagc aaccctggca
     4921 ccaccaccca catcgccatc tgcaccgcgt gcagcacgaa cacgcccagg tccagccgcc
     4981 acaggtgggg atggcgccac acctcctgca accgcccgcg cggcgcatcg acgtgctgca
     5041 ccggttctgc cggcgtggcc accagcacca gcaccacacc ggccagcgcc agcgccgccg
     5101 tcagccagaa cagcccggac aggccgatcg cgccggtgag cggcggcgcc gccatcagcg
     5161 acagcgcaaa catggcggcg atgctgatgc cgaccagcgc catcgccttg gtgcgcacct
     5221 cgtcgcgcgt caggtcggcc agcagcgccg tcactgccgc cgacacggca cccgcaccct
     5281 gcagcgcgcg cccggccacc agcatgtgca cgctgccggc cagcgccgcc agcgcgctgc
     5341 ccagcgcaaa caccagcaac ccggccacga tcaccttctt gcgaccgatg cgatcggacg
     5401 ccagcccgag cggcacctgc atcaccgcct gcgtcaggcc atagatgccc atggccagac
     5461 ccaccagtgc cgggtcgtcg ccgcccgggt agcgcgccgc ctccagcgcg aacaccggca
     5521 gcaccagaaa cagtcccagc atgcgcaggg caaagatacc ggccagcgac aggctggcgc
     5581 gccgctcgtc ggcggtcatg cggtggacgt gggatgcgtg ggcagcaggc gtggcggagg
     5641 cggacatcga caaacggcgc agaaaacgac gccggctgtg cgccggccag cggtattgtc
     5701 ggtgatcgtg gatctgctag attgccggat catgaaagcc acccaccacc tgccccgcgc
     5761 caccgcagtg ctggcgctgg cctgcaccct gcttgccgcc tgtgcccagc agcccgccgc
     5821 ccccaacccc tcggccgccc aggaactgaa ggcgtttccc gccaccctga aaggccacca
     5881 gcgccacgtg atcgagctgc cggcgctgcc cgatgaggcc gatcgccaga tcgagctgat
     5941 cggcaccaag gccatgacgg tggactgcaa cacccgtggc atggacggcc gcttcgagtc
     6001 gcgtgacgtg cagggctggg gctacaacta ctgggtgttg caaagccgcg gccaggtgat
     6061 gggcaccatg atggcctgcc cgcccggcag ccagcgcccc ggcgtcgcgc agacccaggg
     6121 cacgctgctg cgctacaaca gcaagctgcc ggtggtggtg ttcgtgcccg agggcatggg
     6181 gctgcgctgg cgcgtgtgga gcgccggcgc catgcaagac gcgtcggctc gctgagagcg
     6241 gccccaactg tcatctttca tagctgctcg cgcttgccag gcaagcgctg cagcctgatt
     6301 cctcttgcag caacacgccc gaccaggcgt gcccgcgcga agctcgtcgt cgcttgccag
     6361 gacgacacgc gcgcactgcg gtcgatcacc gcttgtgggc cggcagcgat ccgcgcagca
     6421 tgcgtgtcac ctctccgcgt cgacacccgg tcgatgcccg tgatggatac acggcacgca
     6481 cacgccgtat cctcggaggg tggcatctgg ccggaggatc ggcatgtcct ttcttcaaag
     6541 cacttccaac accgccttct tcattgcact gggcatcgtt gtgctgctct cgggcatcgg
     6601 ctttggcatc gtggcctggg tcggccgcct taccggtgcg ctgcgcggcc gtgagcctgt
     6661 gcccatcaac atgctgacgg aaggctacct gttggcgcga ggcaaggcca gtggacggac
     6721 gttgcgggcg ccgctgtcgg gccgtccgtg tgtgtggtgg gagatgaagg tgtgggagcg
     6781 ccggatcgaa atcgaccgtc aagccagcag cgacgaaccc gatcggcgcg cctcctggac
     6841 cttgctgcga cacgacgtgt cgcggcgccc catccattgc actcaaggct tcgtgacttg
     6901 caccgtgcag cccgatggca tgaccctgac cgtcccctcc gaggtgcgcg actggaaggg
     6961 caagcaatat ccgccggaga accgcgaccc gccagtgcag cccggttcgg cgttctccta
     7021 tcggcaggtg cacgatacgg gcttcaccat cgtcggtggc aaacttttgg gcgaccgcta
     7081 tcgctacgtc gaactgctca tcgcgtcgaa cgccgagctg tttgttctcg gtcgtgttga
     7141 acgcgtggca gcccgggcag gggcggccag cacgcatgac gatgcgccgg cacccgaggg
     7201 cgaatccgtt gccccgttcg agcaggcgca acgggaggag aacgaggacg aacagatgag
     7261 cccgagcatg cgcgaggtcc agtggcgcat cgcgccggaa aagggtcgtc cctacatcgt
     7321 ggccacgcag ccgccggagc agtggatggc cacgatcaag ctggccagtc agggcggcct
     7381 gatcgtgggc accgtgacgg cagcgttggc agccttcatg ctctgggcgc gctatcaggg
     7441 cggctgaacc cagcaggccg atggcgtcgg tgctctggcc acccgccggg ccgaggcatc
     7501 cccggcaggc tcagtttctg gcgaatgtct tggccagcgg tgcgtcgaac gtcacgctgc
     7561 cggtggcgcc cacatcgcct ttgagcgtcc agctgccggc caggcgctgg tggctgtcct
     7621 gcttgagctg catggtggcc ggctgggcgg tgtcggagtg ctgcaccatc tggcccttgc
     7681 cgacgaacca gtagcctagg cgcgggccgc cgtcgaaatc caccgtgacg ccgctgtgca
     7741 gatcggaact cacgcagccc agccgatcgc acttctggat cgcggacgtc aggtcgacct
     7801 ctgacagcac cagcctgcgc atgggcttgc catcgaatga ggggccggcc agcaaatagg
     7861 catagcgcgg cgcgaccttg accacgccgg agcgcgcctg cagggccaac tcgccagacg
     7921 cctgctgcgc ctgcgcgcct gctgccacca gcgtgaccag agccaggccg atcgcggccc
     7981 gacgggtggc cgatcgcaag ctgccgcaat gcatggatct catggcgatc tcctcggggg
     8041 tcagggcagc cgctcgcgcc tggcgcgcgt gggcaaggcg tcgcagcggc aggcaggaat
     8101 gctacatcgg atcggaaccc tgcggtgggc gaatctgggc ttcatggtgt gcgggccggg
     8161 tgtcttgcgc cacggatcgg ccgcatgcaa gaagcaaccc agacagcggc caggtagggc
     8221 gtgagcaggc accaacgccc cgatttcgga taatggccgg ttgccctgcg cccggggccg
     8281 tccgccatga acgatctcgc cgcccctgat ttgtccgacg ccgaaggcct gccgctggcc
     8341 accgcactgc gtcagccgcg catcagcgtg cgcggagcgc gcacgcacaa cctcaagaac
     8401 atcgacatcg acattcccaa ggaccggctg gtggtgatca ccggcctgtc gggctcgggc
     8461 aagtcgtcgc tggccttcga cacgctgtac gccgagggcc agcgccgcta tgtcgaaagc
     8521 ctgtcggcct atgcgcggca gtttctgcag ctgatggaca agcccgacgt cgacctgatc
     8581 gagggcctgt cgcccgccat cgccatcgag cagaaggcca ccagccacaa cccgcgctcg
     8641 accgtgggca cggtgaccga gatccatgac tacctgcgcc tgctgtttgc gcgcgccggc
     8701 acgccccact gccccgaaca cgacctgccg ctcacggtgc agaccgtcag ccagatggtc
     8761 gacgccacgc tggccctgcc gcaagacacg cgcgtcatgg tgctggcgcc gctggcgcgc
     8821 gcacgcaagg gcgaattcag cgagctgctg gcgcaaatgc aggcgctggg ttacgtgcgc
     8881 tttcgcatcg acggccaggt ggtcgaggcc gatcgcctgc cggtgctgca gaagaacgac
     8941 aaacacgaca tcgacgtggt gatcgaccgg ctgaaggtgc gcggcacggc cgacggcccg
     9001 ggcggtgcgg caaccgccct ggccgcgcgc ctggccgaca gcttcgaagc cgccctgcgc
     9061 ctggccgacg gccgcgcgat cgcgctggag atggacgagg gaaaggaaca cctgttcaac
     9121 agcaagttcg cctgcccggt gtgtggccac tccctgcccg agctggagcc gcggctgttt
     9181 tcgttcaacg ccccggccgg cgcctgcccg acctgcgacg gcctgggcac caccgacgtg
     9241 ttcgaccccg aacgcgtggt ggccttcccc tcgctcagcc tggccagcgg cgccatcaag
     9301 ggctgggacc ggcgcaacgg ctactacttc gcgctcatcg aaagcctggc caagcactac
     9361 aggttcagcg tcgagacgcc gttcgaggag ctgcccgagg cggtgcgcca cgtcatcctg
     9421 cacggctcgg gcgatgaggc gctccggttc agctacacct tcgacagcgg cgcgcaggcc
     9481 ggccgcaagg tcagcaagaa gcacccgttc gaaggcatcc tgcccaacat ggcgcggcgc
     9541 tggcgcgaga ccgactccaa cgtggtgcgc gaagaactgg cgcgctaccg cagccagcag
     9601 ccctgcaccg cctgcggtgg cacgcggctg cgccaggagg cgcgccacgt gttcgttggc
     9661 gagggcgcgc agcggcgcgc catctaccag atcagccatg tcacgctggg cgaggcgcag
     9721 gcctacttcg atgcgctgcg actgacgggc gccaaggccg acatcgccga gcgggtggtg
     9781 cgcgaaatcg ccagccgcct gaagttcctc aacgatgtcg gcctgggtta ccttagcctg
     9841 gagcgcagcg ccgacacgct gagcggcggc gagtcgcagc gcatccgcct ggccagccag
     9901 atcggctcgg gcctgagcgg cgtgatgtac gtgctggacg aacccagcat cggcctgcac
     9961 cagcgcgaca acgaccgcct gatcgccacc ctgcagcacc tgcgcgactt gggcaacacg
    10021 gtgctggtgg tcgagcacga cgaggacatg atgcgtgcgg ccgaccacat cgtcgacctc
    10081 ggtcccggcg ccggcgtgca cggcgggcgc gtgatggcgc agggcacgtt cgcgcaggtg
    10141 gcggccacac ccgcttcggt gaccggccag ttcctgcgcg gcgaacgggc gatcgccgtg
    10201 cccagccgcc gtcgacgctc acatatcgat agctgctcgc gcacaccgga cgggcgtcag
    10261 agcccggaaa cacttgaaac gcaccgcccc accccgagcg gcgtgctgcg catcgtcggc
    10321 gcccgtggcc acaacctgaa ggacgtcacg gtggacgttc cggtggggct gctgacctgc
    10381 gtcaccggcg tgtcgggctc gggcaagagc accctggtca acgacacgct gtacacggcg
    10441 gccgcgcgcg aaattcaccg tgcccacgcc gaaccggcgc cgcacgaggc catcgagggg
    10501 ctggcgcagt tcgacaaggt gatcgcggtc gatcagtcgc ccatcggccg cacgccgcgc
    10561 agcaacccgg ccacctacac cggcctgttc accccgatcc gcgaactgat ggccgaaacc
    10621 gccatggcgc gcgagcgcgg ctggggcccg gggcgcttca gtttcaacgt gccgcaggcc
    10681 agcgggggcg gccgctgcga ggcctgccag ggcgacggcg tggtgaaggt cgaaatgcac
    10741 tttctgcccg acgtctacgt gccctgcgac gtctgtgccg gccagcgcta caaccgcgaa
    10801 acgctgcagg tgcagtggaa gggccgcaac atcgcccagg tgctggacat gacggtggag
    10861 gccgcgcacg ccttctttgc cgaccagccc gccatcgcgc gcaagctgca gaccctgctg
    10921 gacgtgggcc tgtcctacat caccctgggg cagagcgcca ccaccttgtc gggcggcgag
    10981 gcgcagcgcg tcaagctggc gctggagctg tccaagcgcg acaccggccg caccctgtac
    11041 gtgctggacg agccgaccac cggcctgcac ttcgccgacg tcgagctgct gctcaaggtg
    11101 ctgcaccagc tggtcgaggc cggcaacacc gtggtggtga tcgagcacaa cctggacgtg
    11161 atcaagacgg cagactggct gatcgacatg ggcccggagg gcggtgatgg cggcggcacg
    11221 gtggtggcgc agggcacgcc ggagcaggtg gcgggccatc cggacagcca caccgggcgc
    11281 tacctcaagc cggtgctggt ggccgccgcg gcggcgtagc cgcacgcca
//
LOCUS       DAOOOG010000076        11282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6472_length_11282_cov_24.675960, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000076 DAOOOG010000000
VERSION     DAOOOG010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6472_length_11282_cov_24.675960"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PK242_07205"
     CDS             <1..288
                     /locus_tag="PK242_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HOZ93841.1"
                     /translation="HVLPTLQETFPGIDWSAQGAYFFSRVAQHGQRRAEEMRESARTV
                     QEAGFEPLMTAAIAAKQQWVADQARAGAFKPIVKGERWQDFADALLRQRQA"
     gene            301..1245
                     /locus_tag="PK242_07210"
     CDS             301..1245
                     /locus_tag="PK242_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOZ93842.1"
                     /translation="MLDILAITGPIYLCVALGWLTTRAGLFARADMRVLGQFVLYIAL
                     PALLFGAVGQRPIADILDWHYMAVFALGGLATIGLGLFWARRLAGQAPLAGVVQAMGM
                     SCPNSGFVGYPINLLVLGGPTAGVILGMNMLVENLLLIPLLLALADSASGGGHWRAVL
                     RGVAVGLWRNPLVLGLAAGLAAALLGWRLPGPLERTVGLLAQSSAALSLFVIGGSLAG
                     LRLAGMGRTVARIAAGKLIAHPLLTLGALWLLAALGAPLSDPALKKGVVLATACPMFG
                     IYPILAGRFGAEGLAAAALLGTTVASFFSISALLWWLG"
     gene            complement(1448..1831)
                     /locus_tag="PK242_07215"
     CDS             complement(1448..1831)
                     /locus_tag="PK242_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOZ93843.1"
                     /translation="MTHRILADRIRLKRAYEPASADDGARILVERLWPRGLSKQTLAL
                     TEWARDVAPSTALRQWYGHEPTRWDGFRQRYAAELRQQPAALEALRQRARAGVVTLIY
                     AARTDTLNNAVALREFLLHPERLEP"
     gene            complement(1958..2935)
                     /locus_tag="PK242_07220"
     CDS             complement(1958..2935)
                     /locus_tag="PK242_07220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HOZ93844.1"
                     /translation="MHAWLCENPVGVDALTWQELPTPQPGKGQVLIRIQAASLNFPDL
                     LIVQNKYQFKPELPFVPGSEYAGVVEAVGEGVTELKVGQAVACLSGTGGFATHTLAPA
                     KLCMPLPPGFPPADAAAFIMIYATSHHALMDRGQLKAGETVLVLGAAGGVGMSAIQIA
                     KIAGARVIAAASTEEKLAFCRQLGADDTINYGTQDLRERLKELTGGKGPDVIYDPVGG
                     DFTEPAFRSIAWRGRHLVIGFAAGEIPALKVNLALVKGASIVGVFWGDFARREPQANA
                     AMMQELASWYAQGKIKPLIDRQMPMSELKAAYARMGSRQVQGKLVLVNG"
     gene            3113..3901
                     /gene="surE"
                     /locus_tag="PK242_07225"
     CDS             3113..3901
                     /gene="surE"
                     /locus_tag="PK242_07225"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOZ93845.1"
                     /translation="MRILLCNDDGYQAAGIVAFHRALAALPGVQVEVVAPEHNNSAQS
                     NALTLRAPLYVNQAANGFRYVNGTPADCVHIALTGLLDYRPDLVVSGINNGANMGDDT
                     IYSGTVGAAMEGYLFGIPALAFSLVDKGWAHIDAAAVRAAELVARFGAHPPGGGKPWL
                     LNVNIPNLPLAQIRPPKVCRLGRRHAAQSAITMTSPHGETMYWIGGAGSAADAAEGTD
                     FHATAEGHVAITPLKVDLTDHDALSYWAQGLGHLLAAGAAGGAG"
     gene            3901..4713
                     /locus_tag="PK242_07230"
     CDS             3901..4713
                     /locus_tag="PK242_07230"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOZ93846.1"
                     /translation="MATPPRPGFPARIDLGTGGKSAVRPPARPAGVAGTSANPPLTSA
                     TAPQAASMARDPIPARARMVQLLAAEGIRDPQVLRAMGAVDRHHFVDGALVPQAYEDT
                     ALPIGLGQTISKPSVVARMLELLMQGAVRGPDGRLGRVLDIGTGCGYQAVVLAQLASE
                     VYSIERLRDLHDKARANLRPLRLATVHLILGDGMAGFARGAPYAGIVAAAGGEAVPEA
                     WIDQLAWGGRIVAPVATAAGQQALIVIDKSRQGVQQTVLEAVNFVPLKSGIA"
     gene            4746..5660
                     /locus_tag="PK242_07235"
     CDS             4746..5660
                     /locus_tag="PK242_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOZ93847.1"
                     /translation="MYLTGRRILLAATLLGGVVLAGCSGTPLTPAPVEDRDTRGTPSR
                     VDPATLPGFENAGKPGYYTVRPGDTIMRIATEVNHPWRDIARWNNLENPNVIEVGQVL
                     RVVPPQGATAGAATPITAVPLPAPPVAASGAKPAPPPVPVATQTTPGVSAPAAPTAAP
                     ASAPTPPAPAPAPAAAPPSAGADNVDFIWPASGSVVAGFDEAKNKGLGIGGKAGDPVL
                     AAADGRVVYAGAGLRGYGNLIILKHNNTFLTAYAHNQKLLVKEDQDVRKGQKIAEMGS
                     SDTDRVKLHFEIRRSGKPVDPARYLPAR"
     gene            5787..7226
                     /gene="rlmD"
                     /locus_tag="PK242_07240"
     CDS             5787..7226
                     /gene="rlmD"
                     /locus_tag="PK242_07240"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOZ93848.1"
                     /translation="MNLEPDHSRDAALPEGWLQVDSIDLEAQGIARRPDGKVVFIDGA
                     LAGELVSVSVHRRKNQWEAATLTELHRASSLRVRPRCPHFGLHQGACGGCKMQHLDAS
                     AQVAVKQRALEDQLWHLGKVRPALILRPIEGPAWGYRWRARLSVRYVAKRGEVLVGFH
                     ERKSRYVADMRECPVLPAHVSALLLPLRALIHGMQARDSCPQIELACGDEVTALVLRH
                     LEPLSAADRQRLSEFGRQHGVQWWLQPKGPDSVQRLEPGGPELAYALAEFGLRMPFRP
                     TDFTQVNPHINRVLVGRALRLLQAQRHERVIDWFCGLGNFTLPIATTAGQVLGVEGSA
                     ALVDRARQNHEQNRAAARTGSALAATEFVARNLFDMSAAQLVQDGSADRWLVDPPREG
                     AFALVKALAAIHQGRLGAPDAEPLPAGADGWRPPRRIVYVSCNPATLARDAGLLVHQA
                     GYRCSAAGVVNMFPHTAHVESMAVFDLQD"
     gene            complement(7288..7363)
                     /locus_tag="PK242_07245"
     tRNA            complement(7288..7363)
                     /locus_tag="PK242_07245"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7328..7330),aa:Asn,seq:gtt)
     gene            complement(7438..7800)
                     /locus_tag="PK242_07250"
     CDS             complement(7438..7800)
                     /locus_tag="PK242_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93849.1"
                     /translation="MTHVRTLLTLALPGALLLGACAGGGHPRPGTSTQQEYEAAMRDQ
                     SPVRVIVREGPISNSDVGRTPEELARQEAGQQCGRASLDAKVLDVQRQRAPGRDATGG
                     VVQFERVRLTFRCERGRP"
     regulatory      7831..7939
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8104..9270
                     /locus_tag="PK242_07255"
     CDS             8104..9270
                     /locus_tag="PK242_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ93850.1"
                     /translation="MSPPALSIGIAGAGLLGRLLAWQLSRRGHQVSVFDSAAGPQPVA
                     RSQAGDPYVPTAAGFTAAGMLSPLSELDNAEPAVAHMGFRSLALWPGILAALPGAPEL
                     VVRGSLLLAHRADLGTARRMLDRLHVATATPEWRHLQLPAGEQPLSPAELRTLEPAIQ
                     GPAHAWLLPGEGHIDTVATMIALHAGASGVDWHWGVPVRALQGGDAGGTLLLADGRAL
                     AFDAVVDVRGAGARPELPVRGVRGEVVWLDCPGHGLTRPARLLHPRHFVYMVPRSSRD
                     VVVGASEIESEDRSPVSLRTAVELMAAAHSVLPGLAEARILKLDVNLRPALPDNNPLI
                     EHAGRLLRINGLFRHGWLLAPALVERALAEAPWLDTALASDGRPSRHGAAKAQA"
     gene            9425..9655
                     /gene="thiS"
                     /locus_tag="PK242_07260"
     CDS             9425..9655
                     /gene="thiS"
                     /locus_tag="PK242_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOZ93851.1"
                     /translation="MPSHSSDAATLHILLDGHPHAIPAGSTLADVVASLGHAPEAVST
                     AVNGAFVARPARATQRLAEGDQVLLFQPIVGG"
     gene            9671..11104
                     /gene="arcD"
                     /locus_tag="PK242_07265"
     CDS             9671..11104
                     /gene="arcD"
                     /locus_tag="PK242_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HOZ93852.1"
                     /translation="MNPPKTTKLRVGALTALVVGSMVGGGIFSIPQNTAASAGVGATA
                     IAWLITGVGMLALAFVFLNLALREPALDSGIYAYARASFGPFTGFSSAWGYWFMAVLG
                     NVGYYVLLFSTLGHYWPVFGDGNTPLAVVCASVMLWGTHALVLRGIREAALINQVTTV
                     AKLVPLLLFVLLVALAFQPQVFRADVWGRANPQLGSVLEQVRGMMLVTVWVFIGVEGA
                     SVYSAHAQRRIDVGRATLAGFLFVLALLVAVSLLSMGVMAQPELAGLKNPSMAYVLER
                     AVGHWGGVLISVGLLISLSGALLSWLMLSAETLYSAARDGAAPDWLAGQNARGVPSHA
                     LWLTSACVQAFLLLTLWSNSTYLSLVNLGTAMILLPYTWAAGHALKIALSGARYGVAE
                     GGRRRRDALLSLLALAYALWLVYAGGIKYLLLGALLYAPGALLYAWARRSHGRAVFSR
                     REWLGFLAMLAAAGVAALGLQRGWLSL"
     gene            11138..>11282
                     /locus_tag="PK242_07270"
     CDS             11138..>11282
                     /locus_tag="PK242_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOZ93853.1"
                     /translation="MHDSDHDTWAVGDVRLDSRFLLGTAKYPSPRVLADAIAASGTQI
                     VTVG"
BASE COUNT         1533 a         3879 c         4080 g         1790 t
ORIGIN      
        1 cacgtgctgc ccacattgca ggaaaccttt cccggcatcg actggagcgc gcagggcgct
       61 tacttcttca gccgcgtggc gcagcacggc cagcgccgcg ccgaggaaat gcgcgagtcg
      121 gcgcgcacgg tgcaggaggc gggcttcgag ccgctgatga ccgcggcgat tgccgccaag
      181 cagcagtggg tggccgacca ggcgcgcgcc ggggcgttca agccgatcgt caagggcgag
      241 cgctggcagg actttgccga cgcgctgctg cggcagcgtc aggcctgagg gagccttcgc
      301 ttgctcgaca ttctggccat caccggcccc atctacctgt gcgtcgcgct gggctggctg
      361 accacccgcg ccggcctgtt cgcgcgcgcc gacatgcggg tgctgggcca gttcgtgctc
      421 tacatcgcgc tgccggcgct gctgttcggc gccgtcggtc aacggccgat cgccgacatc
      481 ttggactggc actacatggc ggtgttcgcc ctgggcggtc tggcgaccat cggcctcggg
      541 ctgttctggg cgcgccgcct ggccgggcag gcgccgctgg caggcgtggt gcaggccatg
      601 ggcatgagtt gccccaactc gggcttcgtc ggctacccga tcaacctgtt ggtgttgggc
      661 gggccgaccg ccggcgtgat cctgggcatg aacatgctgg tggaaaacct gctgctcatc
      721 ccgctgctgc tggcgttggc cgacagcgcc agcggcggcg gccactggcg cgcggtgctg
      781 cgtggtgtgg cggtgggctt gtggcgcaac ccgttggtgc tgggtctggc ggccgggctg
      841 gcggcggcgc tgctgggttg gcggctgccg ggcccgctgg agcgcaccgt gggtctgctg
      901 gcgcagtcga gcgcggcgct gtcgctgttc gtcatcggcg gctcactggc cgggctgcgg
      961 ctggccggca tggggcgcac ggtggcgcgc atcgccgccg gcaagctgat cgcgcacccg
     1021 ctgctgaccc tgggggcgct gtggctgctg gcagcgctgg gtgcgccgct gagcgacccg
     1081 gcattgaaga agggcgtcgt gctggccacc gcctgcccga tgttcggcat ctacccgatt
     1141 ctggccggcc gctttggcgc cgaaggcctg gccgccgccg cgctgctggg caccacggtg
     1201 gcgtcgttct tcagcatcag tgcgctgctg tggtggctgg gatgaggcgc cgcaccgggt
     1261 tcagatgccg gtctggcgcc gcccatgcgt ggccatgccc cgtgtcggga gtttggagtc
     1321 gggcccgtcc agatggcctt cagaaccagc agagcgacga gggggtccgc cggccgcgaa
     1381 tggttcatgc gtcgtgcggg acgcagccgt tgtcatgggc cattcgccat tcttctgaac
     1441 agcggcatca gggttcgagc cgctccgggt gcagcaggaa ttcacgcagc gccacggcgt
     1501 tgttcagcgt gtcggtgcgt gcggcataga tcagcgtgac gacgccggcg cgagcgcgct
     1561 ggcgcagcgc ttcgagggcc gcgggttgct ggcgcagctc ggccgcgtag cgctggcgaa
     1621 agccgtccca gcgcgtcggc tcgtggccgt accactgacg caaagcggtg ctgggcgcca
     1681 catcgcgcgc ccattcggtc agcgccagcg tctgcttgct caggccgcgc ggccacaggc
     1741 gctcgaccag gatacgcgcg ccgtcgtcgg ctgacgctgg ttcgtaggcg cgcttgaggc
     1801 ggatgcggtc ggcgaggatc ctgtgcgtca ttgccgggcg gggccgagct cggatgtcga
     1861 gtcaggatac gcgcacgcca gtgaggacgg tgacggcaga tgtattgccc ctttccacag
     1921 aggggcgagg ggccgggcgc tactcagggt tcatcggtca cccgttgacc agcaccagct
     1981 tgccctgcac ctggcgcgag cccatgcggg cataggcggc cttgagctcg ctcatcggca
     2041 tctgccgatc gatcagcggc ttgatcttgc cctgcgcgta ccaggaggcc agctcctgca
     2101 tcatggcggc gttggcctgc ggttcgcggc gcgcgaagtc gccccagaac acgccgacga
     2161 tgctggcgcc cttgaccagc gccaggttga ccttgagtgc cggaatctcg ccggcggcaa
     2221 agccgatcac cagatggcgg ccgcgccagg cgatggagcg gaacgccggc tcggtgaagt
     2281 cgccgcccac cgggtcgtag atcacgtccg ggcccttgcc gccggtcagc tccttcaggc
     2341 gctcgcgcag gtcctgcgtg ccgtagttga tggtgtcgtc ggcgccgagc tggcggcaga
     2401 acgccagctt ctcttcggtg gaggcggcgg cgatcacgcg cgcgccggcg atcttggcaa
     2461 tctggatggc cgacatgcct acgccgccgg cggcgcccag caccagcacg gtttcgcccg
     2521 ccttgagctg gccgcgatcc atcagtgcgt ggtggctggt ggcgtagatc atgatgaagg
     2581 cggccgcgtc ggccggcgga aagcccggcg gcagcggcat gcacagcttg gccggcgcca
     2641 gcgtgtgggt ggcaaagccg ccggtgcccg acaggcaggc caccgcctgg ccgaccttca
     2701 gctcggtcac gccttcgccc accgcctcga cgacgccggc gtactcggag ccgggcacga
     2761 acggcagttc gggcttgaac tggtacttgt tctgcacgat cagcagatcg ggaaagttca
     2821 ggctggcggc ctggatgcgg atcagcacct gccctttgcc aggctggggc gtgggcagct
     2881 cctgccaggt gagggcgtcg acgccgacgg ggttttcgca aagccatgcg tgcattgaat
     2941 agcgctcctt gaggttcgcg gcaagggtga tggggcgccg cggtctgcac tcgatcatag
     3001 gagcatgggc cgggagcgcc atgtcccaac cgtgacggtg aaaatccggc ggcggggcgg
     3061 gaagggcagg cctacaatcg gttgcattcg cgcattcgcg cctgacccgc ccatgagaat
     3121 cctgctctgc aacgacgatg gctatcaggc cgccggcatc gtagcctttc accgggccct
     3181 ggccgcgttg cccggcgtgc aggtcgaggt ggtggcgccg gagcacaaca acagcgccca
     3241 gtccaacgcg ctgaccctgc gcgcgccgct gtatgtgaac caggcggcca acggttttcg
     3301 ctacgtcaat ggcacgccgg ccgactgcgt gcacatcgcc ctcaccgggc tgctggacta
     3361 ccggccggat ctggtggtgt cgggcatcaa caatggcgcc aacatgggcg acgacaccat
     3421 ttattcgggc accgtcggcg cggccatgga ggggtacctg ttcggcatcc cggcgctggc
     3481 gttttcgctg gtcgacaagg gctgggccca catcgacgcc gccgccgtgc gcgccgccga
     3541 gctggtggcg cgttttggtg cccacccgcc gggtggcggc aagccgtggc tgctgaacgt
     3601 caacatcccg aatctgccgc tggcgcagat tcgcccaccc aaggtgtgcc ggctgggccg
     3661 gcgccatgcc gcgcagtcgg ccatcaccat gaccagcccg cacggcgaaa ccatgtactg
     3721 gatcggcggc gccggttcgg ccgccgacgc ggccgagggc accgatttcc acgccaccgc
     3781 cgagggccat gtcgccatca ctccgctgaa ggtggacctg accgaccacg atgcgctgag
     3841 ctactgggcg caggggctgg ggcacctgct ggcggctggc gcggcgggag gggcaggctg
     3901 atggccacgc cgccgcggcc cggctttccg gcgcgcatcg acctgggcac gggcggcaag
     3961 tcggccgtcc gcccgccggc gcgcccggcc ggcgtggcag gcaccagcgc caacccgccg
     4021 ctgaccagcg ccaccgcacc gcaggcggcc agcatggcac gcgacccgat cccggcgcgc
     4081 gcccgcatgg tgcaactgct ggccgccgaa ggcatccgcg acccccaggt gctgcgcgcc
     4141 atgggtgcgg tggaccgcca ccatttcgtc gacggcgcgc tggtgccgca ggcctacgag
     4201 gacaccgcgc tgcccatcgg cctgggccag accatcagca agcccagcgt ggtggcgcgc
     4261 atgctggagc tgctgatgca gggcgccgtc cgtggccccg acggccgcct gggccgcgtg
     4321 ctggacatcg gcaccggctg cggctaccag gcggtggtgc tggcccagct ggccagcgag
     4381 gtctacagca tcgagcgcct gcgcgacctg cacgacaagg cgcgcgccaa cctgcgaccg
     4441 ctgcgccttg ccaccgtgca cctgatcctg ggcgacggca tggccggctt tgccaggggc
     4501 gcgccctatg ccggcatcgt cgccgctgcc ggcggtgagg ccgtgcccga agcctggatc
     4561 gaccagttgg cctggggtgg gcgcatcgtg gcgccggtgg ccacggcggc gggacagcag
     4621 gcgctgatcg tgatcgacaa gtcgcgtcag ggggtgcagc agacggtgct ggaagcggtc
     4681 aacttcgtgc ctctaaaatc cggtatcgct tagcggattt ccagagcgct tcaagaaggg
     4741 tttgtatgta tttgacggga cgcaggattt tgctggccgc cacgctgctg ggcggggtcg
     4801 tgctggcggg ctgctccggc accccactca ccccggcgcc ggtggaagac cgcgacaccc
     4861 gcggcacccc gtcgcgggtc gatccggcca ccttgcccgg cttcgaaaac gccggcaagc
     4921 cgggctacta cacggtgcgt ccgggcgaca ccatcatgcg catcgccacc gaggtcaacc
     4981 acccctggcg cgacatcgca cgctggaaca acctggagaa cccgaacgtg atcgaggtcg
     5041 gccaggtgct gcgcgtggtg ccgccgcagg gcgcaaccgc cggcgcagcc acgccgatca
     5101 ccgccgtgcc gctgcccgcg ccgccggtcg ccgccagcgg tgccaaacct gcgccgccgc
     5161 cggtgccggt ggccacgcag accacgccgg gcgtgagcgc gcccgcggcg ccgacggccg
     5221 cgcctgcgtc ggcaccgacg ccgcccgcgc ccgcacccgc gccagcggcg gcgccgccgt
     5281 cggccggggc cgacaacgtc gacttcatct ggcccgccag cggttcggtg gtggccggct
     5341 tcgacgaagc caagaacaag ggcctgggca tcggcggcaa ggcgggcgat ccggtgctgg
     5401 cggcggccga cgggcgcgtg gtctatgccg gcgccggtct gcgcggctat ggcaacctga
     5461 tcatcctcaa gcacaacaac accttcctga ccgcctacgc ccacaaccag aagctgctgg
     5521 tcaaggaaga ccaggacgtc agaaaaggcc agaagatcgc cgaaatgggc tccagcgaca
     5581 ccgaccgcgt caagctccac ttcgagatcc gccgctccgg caagccggtg gacccggcgc
     5641 gctatctgcc ggcgcgctga ttccgggccc gcgggccggc caagggcgct ccggtgagcg
     5701 cccttcgtgt ttcggcggcc cgtgggttgc tataatatca atagctcctg gcgctcgcca
     5761 gataagcgcc ggagcttctt ttcatcatga acctcgagcc cgatcacagc cgcgacgcgg
     5821 cactgcccga aggctggctg caggtcgatt cgatcgacct cgaagcccag ggcatcgcgc
     5881 gccggcccga cggcaaggtg gtgttcatcg acggtgcgct ggccggcgag ctggtgtcgg
     5941 tcagcgtgca ccggcgcaag aaccaatggg aagccgccac cctgaccgag ttgcaccgcg
     6001 ccagcagcct gcgcgtgcgc ccgcgctgcc cgcatttcgg cctgcaccag ggcgcctgtg
     6061 gcggctgcaa gatgcagcac ctggacgcca gcgcccaggt ggctgtcaag caacgtgcgc
     6121 tggaagacca gttgtggcat ctgggcaagg tcaggccggc gctgatcctg cgccccatcg
     6181 aaggcccggc ctggggctac cgctggcgcg cgcgcctgtc ggtgcgctac gtggccaagc
     6241 gcggtgaggt gctggtcggt tttcacgaac gcaagagtcg ctacgtggcc gacatgcgcg
     6301 agtgcccggt gctgcccgcc cacgtcagcg cgctgctgct gccgctgcgc gcgctgatcc
     6361 atggcatgca ggcgcgcgac agctgccccc agatcgagct ggcctgcggc gacgaggtca
     6421 cggcgctggt gctgcgccac ctggagccgc tcagcgccgc cgaccgccag cgcctgagcg
     6481 agttcggccg ccagcacggc gtgcagtggt ggctgcaacc caagggcccg gacagcgtgc
     6541 agcggctgga gccgggcggc cccgagctgg cctacgcgct ggcggaattc ggtctgcgca
     6601 tgccgtttcg accgaccgac ttcacccagg tcaacccgca catcaaccgc gtgctggtgg
     6661 ggcgggcgct gcgcctgctg caggcccagc ggcacgagcg cgtgatcgac tggttctgcg
     6721 gcctgggcaa cttcacactg ccgatcgcca ccaccgctgg ccaggtgctg ggggtggaag
     6781 gcagcgccgc cctggtcgac cgcgcccgcc agaatcatga acaaaatcgt gctgcagcgc
     6841 gcactggatc tgcgctggca gctaccgaat ttgtagcgcg caacctgttc gacatgagtg
     6901 ccgcgcaact ggtgcaggac ggcagcgccg accgctggct ggtggacccg ccgcgcgaag
     6961 gtgcgtttgc gctggtcaag gcgctggcgg ccatccacca ggggcgcctc ggtgcgccgg
     7021 acgccgagcc gctgcccgcc ggtgccgacg gctggcgtcc gccgcgccgc atcgtctacg
     7081 tcagctgcaa ccccgccacg ctggcgcgcg acgccggcct gctggtgcat caggccggct
     7141 accgctgcag cgccgccggg gtggtcaaca tgtttccgca caccgcgcac gtcgaaagca
     7201 tggcggtgtt cgacctgcag gactgaggcg gtacgccgac aagcaaaagc cccgcacaac
     7261 ttgtgctgtg cggggcttgc tgttctttgg ctccccgacc tgggctcgaa ccagggacct
     7321 acggattaac agtccggcgc tctaccgact gagctatcgg ggaacaagcc tcaaattata
     7381 taccggattt tggcgactgc gccggccccc cggtgcgcat ggctcgcaaa accacactca
     7441 cgggcggccg cgctcgcagc ggaaggtcag gcgcacgcgc tcgaactgga ccacgccgcc
     7501 ggtggcgtcg cgcccgggcg cgcgctggcg ctgcacgtcc agcaccttgg cgtccaggct
     7561 ggcgcggccg cactgctggc cggcttcctg gcgggccagc tcttccgggg tgcgaccaac
     7621 gtcgctgttg gaaatcgggc cttcgcgcac gatcacgcgc accggtgatt ggtcgcgcat
     7681 cgccgcttcg tactcctgct gcgtgctggt gcctgggcgc ggatggccgc cgccggcgca
     7741 ggcgcccagc agcagtgcgc cgggcagggc gagggtgagc agagttctga cgtgggtcat
     7801 ggctggcgcc tcctttccgg ctattatcgg tcgcaactcc ggggtgcccg cgcgagcgag
     7861 ctgagacggc ttggcagcaa gtcgaacccg tcaacttgat ccggttagca ccggcgtaag
     7921 gaagagcagc agcgcgccgc acgccatccc tcggatgcaa cccggcgcag gcggccccgg
     7981 agcccgattg tctgtggccc ttgcaggaga ccgcccgatg catccctccg ctctgatcgc
     8041 tccctggtgg cggcgcgcgc cggcgtcggc cgtgtgccgc ggcacgccag gggcacgtcc
     8101 atcatgagcc cgcccgcgct ttccatcggc atcgccggcg ccggcctgct ggggcgattg
     8161 ctggcctggc agctgtcgcg ccgcggccat caggtgtcgg tgttcgacag cgccgccggg
     8221 ccacagccgg tggcgcgcag ccaggccggc gatccttatg tgcccacggc cgccggcttc
     8281 acggcggccg gcatgctcag tccgctgtcc gagctggaca acgccgagcc ggcggtggcg
     8341 cacatgggct ttcgctcgct ggcgctgtgg ccgggcatcc tggcggcgct gccgggcgcc
     8401 cctgaattgg tggtgcgcgg cagcctgctg ctggcgcacc gtgccgatct gggcaccgcc
     8461 cggcgcatgc tggatcggct gcacgtcgcc accgcaacgc ccgaatggcg ccacctgcag
     8521 ctgccggcgg gcgagcagcc gctgtcgccg gccgagctgc gtacgctgga gcccgccatt
     8581 cagggcccgg cccatgcctg gctgctgccg ggcgagggcc acatcgacac cgtggcgacc
     8641 atgatcgccc tgcacgccgg tgcgtccggc gtggactggc actggggcgt gccggtgcgg
     8701 gcgctgcagg gcggcgacgc tggcggcacg ctgctgctgg ccgacggtcg cgcgctggcg
     8761 ttcgacgcgg tggtcgacgt gcggggtgcc ggtgcgcggc ccgagttgcc ggtgcgcggc
     8821 gtgcgcggcg aagtggtgtg gctggactgc ccgggccatg gcctgacgcg cccggcgcgc
     8881 ctgctgcatc cgcgccactt cgtctacatg gtgccgcgct cgtcgcgcga cgtggtggtc
     8941 ggtgcgagcg aaatcgaaag cgaagaccgc tcgccggtca gcctgcgcac cgcggtcgaa
     9001 ctgatggccg ccgcgcacag cgtgctgccc ggtctggccg aggcgcgcat cctcaaactc
     9061 gacgtcaacc tgcgcccggc gctgcccgac aacaacccgc tcatcgagca cgccggccgg
     9121 ctgctgcgca tcaacggact gtttcgccac ggctggctgc tggcgccggc gctggtcgag
     9181 cgcgcgctgg ccgaggcgcc ctggctggat accgcgttgg cgtccgatgg gcgaccgtcc
     9241 cgccacggtg cggcaaaggc gcaggcctga tgagcgtgtc atgaaaacac cccgcgcgta
     9301 cgcagaagcc gcaaagcttg cgcagaatgc gcagacttcg cagatgcgcc agaaagcgcg
     9361 gcttgcgact tctgcgcaag ccttgcgtct tctgcgcagg gatgtctgaa acccgccgac
     9421 ccccatgccc agccattcat ccgacgccgc cacgctgcac atcctgctcg acggtcatcc
     9481 acatgccatt ccggccggca gcacgctggc cgacgtggtg gcgtcgctgg ggcacgcgcc
     9541 cgaggccgtc agcaccgcgg tcaacggtgc cttcgtggct cgaccggcgc gcgccacgca
     9601 gcggctggcc gagggcgatc aggtgctgct gttccagccc atcgtcggcg gctgataccg
     9661 gctggtctgc atgaatccgc cgaagacgac caagctgcgc gtgggcgcac tcaccgccct
     9721 ggtggtgggc tcgatggtgg gcgggggcat tttttcgatc ccgcagaaca ccgcggccag
     9781 cgccggtgtc ggtgccacgg cgatcgcctg gctgatcacc ggcgtgggca tgctggcgct
     9841 ggccttcgtg ttcctgaacc tggcgctgcg cgagcccgcg ctggattcgg gcatctacgc
     9901 ctacgcgcgc gccagcttcg ggccgttcac cggcttcagc tcggcctggg gctactggtt
     9961 catggcggtg ctgggcaacg tgggctacta cgtgctgctg ttttccaccc tggggcatta
    10021 ctggccggtg tttggcgacg gcaacacgcc gctggcggtg gtctgcgcct cggtcatgct
    10081 gtggggcacg catgcgctgg tgttgcgtgg catccgcgag gcggcgctga tcaaccaggt
    10141 gacgacggtg gccaagctgg tgccgctgct gctgttcgtg ctgctggtgg cgctggcctt
    10201 tcagccccag gtgttccggg ccgatgtgtg gggccgtgcc aacccgcagc tgggcagcgt
    10261 gctggagcag gtgcgcggca tgatgctggt gacggtgtgg gtgttcatcg gtgtcgaagg
    10321 cgccagcgtg tattcggccc acgcgcagcg acgcatcgac gtcgggcgtg ccacgctggc
    10381 gggctttctg ttcgtgctgg cgctgctggt ggcggtgagc ctgctgtcga tgggcgtgat
    10441 ggcgcagccc gagctggccg gcttgaagaa cccgtcgatg gcctatgtgc tggagcgcgc
    10501 ggtcgggcac tggggcgggg tgctgatcag cgtcgggctg ctgatttcgc tgtcgggggc
    10561 gctgctgtcc tggctcatgc tgtcggccga gacgctttat tcggccgcgc gcgacggcgc
    10621 ggcgccggac tggctggccg gtcagaacgc gcgcggcgtg ccgtctcacg cgctgtggct
    10681 gaccagcgcc tgcgtgcagg cctttctgct gctgacgctg tggagcaatt cgacctacct
    10741 gtcgctggtg aacctgggca cggcgatgat tctgctgccc tacacctggg ccgccggcca
    10801 tgcgctgaag atcgcgctca gtggcgcgcg ctacggcgtg gccgaaggcg gccggcgccg
    10861 gcgcgacgcg ctgctgtcgc tgctggcgct ggcctatgcg ctgtggctgg tgtatgcggg
    10921 cggcatcaag tacctgttgc tgggcgcgtt gctgtacgcg cccggcgcac tgctgtacgc
    10981 ctgggcgcgg cgctcgcacg gccgggcagt gttctcgcgc cgcgagtggc tgggctttct
    11041 ggccatgctg gcggccgccg gtgtggcggc gctgggtctg cagcgcggct ggctgagtct
    11101 ctgagaactt gcggacaagc accaaggatg gaccgatatg catgacagtg accacgacac
    11161 ctgggcggtg ggcgacgtgc ggctggacag ccgctttctg ctgggcacgg ccaagtaccc
    11221 gtcgccccgg gtgctggccg acgccatcgc ggcctcgggc acgcagatcg tcaccgtcgg
    11281 cc
//
LOCUS       DAOOOG010000077        11234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6515_length_11234_cov_26.560247, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000077 DAOOOG010000000
VERSION     DAOOOG010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6515_length_11234_cov_26.560247"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1278
                     /locus_tag="PK242_07275"
     CDS             97..1278
                     /locus_tag="PK242_07275"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOZ93854.1"
                     /translation="MEDIVIVSAARTAVGKFGGSLAKVPATELGSIAIRAALERAKVG
                     LDQVGEVILGQVLAAGCGQNPARQALMKAGIAKETPAMTINAVCGSGLKSVMLAAQAV
                     ATGDSEIVVAGGQENMSASPHVLLGSRDGQRMGDWKMIDSMIVDGLWDVYNQYHMGIT
                     AENVARQEGITRDMQDALALASQQKAAAAQAAGKFKDEIVPVSIPQRKGDPVVFATDE
                     YINTKTSAEALAGLRPAFDKAGSVTAGNASGINDGAAAVVVMSARKAAALGLKPLARI
                     ASYATSGLDPATMGLGPVPASQKALQRAGWKVGDVDLFELNEAFAAQACAVNKLLGAD
                     PTKVNVNGGAIAIGHPIGASGCRILVTLLHEMQRSGAKKGLAGLCIGGGMGVAMALER
                     A"
     gene            1412..2149
                     /gene="phbB"
                     /locus_tag="PK242_07280"
     CDS             1412..2149
                     /gene="phbB"
                     /locus_tag="PK242_07280"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HOZ93855.1"
                     /translation="MSQKVAYVTGGMGGIGTAICHRLHKAGFKVIAGCGPTRDYQKWL
                     DEQKALGFTFHASVGNVSDWQSTTDAFTKAKAEHGPIDVLVNNAGITRDRMFLKMTRE
                     DWDAVIDTNLNSMFNVTKQVVPDMVERGWGRIIQISSVNGEKGQAGQTNYSAAKAGMH
                     GFTMALAQELASKGVTVNTVSPGYIGTDMVRAIKPEVLEKIVATIPVKRLGTPEEIGS
                     IVAWLAGEDSGFTTGADFSCNGGLHMG"
     gene            2316..3332
                     /gene="dusA"
                     /locus_tag="PK242_07285"
     CDS             2316..3332
                     /gene="dusA"
                     /locus_tag="PK242_07285"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HOZ93856.1"
                     /translation="MPEAVSPWRLSVAPMLDWTDKHCRFLHRLLSRRTRLYTEMVTTG
                     ALLHGAERRHLRFDAVEHPLALQLGGSEAADLAACARIGARWGYDEINLNCGCPSERV
                     QRGAFGACLMAEPQLVADGVKAMVDAVDVPVSVKHRIGIDREESYDFVRDFVGTVAEA
                     GCRVFIVHARNAWLQGLSPKENREIPPLRHALVYRLKRDFPQLTIVLNGGVTSDAQIT
                     EHLQHVDGVMLGREAYHHPWMMSRWDAEFLGGAACALTQDEVEAEMVAYMARELAEDG
                     TPWSAIARHMLGLHHGRRGARLWRQVWSDHRLKARPAAEVAALARSHLAAGAHAGQGA
                     VAGA"
     gene            complement(3796..4206)
                     /locus_tag="PK242_07290"
     CDS             complement(3796..4206)
                     /locus_tag="PK242_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HOZ93857.1"
                     /translation="MSFAKGLATLAAGALFGFGLALSSMIRPEVVLAFLQGRDLGLAL
                     VMGGGVAVTLLAYKLLPRLLQRPLLGGSFGVHPSSWNRDTLRGAALFGVGWGLTGVCP
                     GPAIAGLGAGNLSLLWAVAGMALGALVQGWRADR"
     gene            complement(4203..4676)
                     /locus_tag="PK242_07295"
     CDS             complement(4203..4676)
                     /locus_tag="PK242_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOZ93858.1"
                     /translation="MSILQTPFPMGWQHYLLGGLCLGAGVALLFLFTGRIGGMSTVFS
                     STWSYVVRRPFFQQARLVDTRGWRLLYALGLIAGALAWWLWRGAGVAEGTQVPWWLLL
                     AGGFVAGYGARLGNGCTSGHGICGLGSLQWPSALAVLTFMGTAIITANLVTRWIV"
     gene            complement(4767..5171)
                     /locus_tag="PK242_07300"
     CDS             complement(4767..5171)
                     /locus_tag="PK242_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOZ93859.1"
                     /translation="MLANLTPFLLHWAITAFALWVAAHVFKGLRFDGGGALVVAALLL
                     GLANAVVRPLLVLLTLPLTLLSFGLFLLVINALVLMLVARLVRGFHLDGFWTAFWASI
                     FMSLLSIVLGLVLGGTPEFTIETMPAPGQRWL"
     gene            complement(5252..7075)
                     /locus_tag="PK242_07305"
     CDS             complement(5252..7075)
                     /locus_tag="PK242_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOZ93860.1"
                     /translation="MSSPNDTAAAEAAKPSHFIRQAIEHDLQAGTYALRRWGGQPGDG
                     PSHQAGMADPARIRTRFPPEPNGYLHIGHAKSIWLNFSLAQEYDGVCHLRFDDTNPEK
                     EEQEYVDSIRDMVRWLGWDHHFADDPARPGVRQPHEYFASDYFDFMYRAAEHLIEAGH
                     AYVDEQSAEQIRASRGDFGTPGTDSPFRHRTPAENLARFREMRDGQHPDGAMVLRAKI
                     DMASPNINLRDPAIYRIRHATHHNTGDKWCIYPMYTFAHPIEDALEQITHSICTLEFE
                     DQRPFYDWLLERLCEGGLLTAPPPRQYEFARLNVGHVITSKRKLRQLVEEGHVAGWDD
                     PRMPTLAGLRRRGYTPDALRLFAERSGVTKTSNAWTDYAALDAALRETLDPVAPRAMA
                     VLEPLKLVIENWTEVFGSPDHLEPCHAPVHPHHPELGQRQFMLGRELWIERSDYEDTP
                     PKGFFRLFPGNKVRLKYGYVIECLGAIKDEAGRASGVSARLIKDTKSGTPGADSVKVK
                     GVITWVAAADGLPAEVRLYDRLFTEEQPDAGGRDFLSVLNPRSLTTVQAIVEPSLAGA
                     QPEDRYQFERHGFFVADRAEHVAGDRLVFNRVTGLKDSWGK"
     gene            7331..7660
                     /locus_tag="PK242_07310"
     CDS             7331..7660
                     /locus_tag="PK242_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93861.1"
                     /translation="MTGLLAAGTLGVTGVAQARSDVYWSVGVGSPGVAIGVGNLPPPV
                     YVAPQPVYVAPPPVYMRPRPVYVAPPPAYYAPPHVVYRPAPRHYWGDGHHHYKHRRHH
                     RHGRDWD"
     gene            complement(7761..8612)
                     /locus_tag="PK242_07315"
     CDS             complement(7761..8612)
                     /locus_tag="PK242_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ93862.1"
                     /translation="MSTHALTALIAEDEPLLAAALQAELARAWPELRVVASVSDGISA
                     VREALALKPDLLFFDIRMPGQSGLDAAAELMDAWDEAAAPFPALVFVTAYDQYAVQAF
                     EAQAMDYLLKPVQPARLEKSVQKLRLALDGRARTATESGASALTDASAAQLQRLLAAL
                     QPALQPAAPRAAWLRQLPVSPAGSGGNTIRMVQVAEVLLLQAADKYVRVVTASGEHLL
                     RTPLKDLLEQLDPAEFWQIHRSTAVRADAIDSVQRDEAGRLHLSLRGCGERLVVSRLY
                     AHRFRAL"
     gene            complement(8609..10177)
                     /locus_tag="PK242_07320"
     CDS             complement(8609..10177)
                     /locus_tag="PK242_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOZ93863.1"
                     /translation="MNWDTIVIGSGIGGLTAAAALAHCGQRVLVLEQHDSLGGMTQTF
                     ERRGYSFATGLHYLTGVTTPEGGEGRLARLLNVLGDGTLRFRPLPRQFDLVRLVHADG
                     GETRFAYGAPEADNWARLKAQHPAEAGAIDAYAARYARARRSSNILMALHGMPRPAAA
                     LLRALLGPWLRRRAGETLGQALAGIDGADLRALLAARGGDYGLPPAQAPLVLHALVMG
                     SYADGGAGYPVGGPARLADSLAAAVRAAKGALRTGARVTQLLAEGGRVVGVRLAEGGV
                     ERARHVVSAMGALNTVQALPAGLAPDWRDALRRHLPSHAYITLYLGLEGNAEALRALG
                     LDGANHWIYDGQALGCAPDPEALLWQCPTDTDAPALYVSFGGLNDPEQAHAPTAEVIA
                     LCDWRFFATWRDSSLGQRPPDYEATKEWIEDRLLSQFKRLFPALKNRIAYHELSTPLT
                     QAAYALAPEGAMYGLACTPARLLDPALHVRTPVPGLLLAGQDVASLGIEGAAMGGFMA
                     AATLKPALWKLMKP"
     gene            complement(10174..11232)
                     /locus_tag="PK242_07325"
     CDS             complement(10174..11232)
                     /locus_tag="PK242_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ93864.1"
                     /translation="MSIAWRDVGRHFLLVVAFCCVIAVLTTGIWPGHGYAAQLGHALC
                     IGLVTWLTIEFGRYAVDERHCHPSLEGGHGWPRGWRALLLTAIGIAAGFTLGEALGRR
                     LFLGADLRSARDDQLGLLITITAGAVGSFFFHARGRAAALAAELARAERDAGEARLVL
                     LQSQLEPHMLFNTLANLRALIGVDPQAAQQMLDRLDAYLRATLAASRSAQHPLAAEFA
                     RLDDYLALMAVRMGPRLAVALDLPDALAALPVPPLLLQPLVENAIRHGLEPRIEGGRI
                     EVSARRTGDALVLTVSDSGVGFDMTEPAAGAGGFGLAQVRERIATAYGGRARVDVQSR
                     PGGGTTVRIRLPMSGATS"
BASE COUNT         1756 a         3911 c         3956 g         1611 t
ORIGIN      
        1 ctgagcgggc tcgtcgaagc atcccgatta tcaaccccat cgggctacag cgcccgtcca
       61 gcaagcgcaa gcagctataa acaggagagc aatcgcatgg aagacatcgt catcgtttca
      121 gccgcccgca ccgccgtggg caagttcggc ggctcgctgg ccaaggtgcc ggccaccgag
      181 ctgggcagca tcgccatccg ggccgcgctg gagcgcgcca aggtgggcct ggaccaggtc
      241 ggtgaagtca tcctggggca ggtgctggcc gccggctgcg gccagaaccc ggcgcgtcag
      301 gccctgatga aggccggcat cgccaaggaa acaccggcca tgaccatcaa cgccgtctgc
      361 ggctcgggcc tcaagagcgt gatgctggcc gcgcaggccg tggccaccgg cgacagcgaa
      421 atcgtggtgg ccggcggcca ggaaaacatg agcgccagcc cgcacgtgct gctgggcagc
      481 cgcgacggtc agcgcatggg cgactggaag atgatcgact cgatgatcgt cgacggcctg
      541 tgggacgtct acaaccagta ccacatgggc atcacggccg agaacgtcgc tcggcaggaa
      601 ggcatcacgc gcgacatgca ggacgcgctg gccctggcca gccagcagaa ggccgccgcc
      661 gcccaggccg ccggcaagtt caaggacgag atcgtgccgg tcagcatccc gcagcgcaag
      721 ggcgacccgg tggtgttcgc caccgacgag tacatcaaca ccaagaccag cgccgaggcc
      781 ctggccggcc tgcgtccggc cttcgacaag gccggcagcg tgacggccgg caacgccagc
      841 ggcatcaacg acggcgccgc cgcggtggtg gtgatgagcg cccgcaaggc ggcggccctg
      901 ggcctcaagc cgctggcgcg catcgccagc tatgccacca gcggcctcga tccggccacc
      961 atgggcctgg ggccggtgcc ggcctcgcag aaggcgctgc agcgtgccgg ctggaaggtg
     1021 ggcgacgtcg acctgttcga gctcaacgaa gcctttgccg cccaggcctg cgccgtcaat
     1081 aagctgctgg gcgccgatcc gaccaaggtc aacgtcaatg gcggcgccat cgccatcggc
     1141 cacccgatcg gtgccagcgg ctgccgcatc ctggtgacgc tgctgcacga aatgcagcgc
     1201 agcggcgcca agaaggggct ggctggcctg tgcatcggcg gcggcatggg cgtggcgatg
     1261 gcgctggagc gcgcctgagc ctgcaggcgg cgcgggcggc tggcgtaaac cctggggttc
     1321 cgccgcctgc gctttgagtt agattcgctg ctgtcggcgc aggatatacg caggcagcta
     1381 ttattttcat agtcccttga ggagacattc catgagtcag aaagtagcat acgtcaccgg
     1441 cggcatgggt ggcatcggta ccgccatctg ccatcgcctg cacaaggccg gcttcaaggt
     1501 catcgccggc tgcggcccca cgcgcgacta ccagaagtgg ctggacgagc agaaggcgct
     1561 gggcttcacc ttccacgcga gcgtcggcaa cgtcagcgac tggcagtcca ccaccgacgc
     1621 cttcaccaag gccaaggccg agcacggccc gatcgacgtg ctggtcaaca acgccggcat
     1681 cacgcgcgac cgcatgttcc tcaagatgac gcgcgaggac tgggacgcgg tgatcgacac
     1741 caacctcaac agcatgttca acgtcaccaa gcaggtggtg cccgacatgg tcgagcgcgg
     1801 ctggggtcgc atcatccaga tcagctcggt caacggcgaa aaaggccagg ccggccagac
     1861 caactactcg gccgccaagg ccggcatgca cggcttcacc atggcgctgg cgcaggagct
     1921 ggcgagcaaa ggcgtcaccg tcaacaccgt cagccccggc tacatcggca ccgacatggt
     1981 gcgcgccatc aagcccgagg tgctggaaaa gatcgtcgcc accattccgg tcaagcgcct
     2041 gggcacgccg gaagaaattg gctccatcgt ggcctggctg gcgggcgagg actcgggctt
     2101 caccaccggc gccgacttct cctgcaacgg tggtctgcac atgggttgat gaccgcgcgc
     2161 ggcgcgctcg acccaccccg ctgcggcggg gttttttgtg tctggtaggg cggtctcggg
     2221 ccatccgacg ccgaaccacc ggggggcacc gcgtggcagg ccgcgcccgg agcgctcggg
     2281 caccgtaccg gcccggcggg gataatcgcc ctcccatgcc tgaagccgtt tcgccctggc
     2341 gcctgtcggt cgccccgatg ctcgactgga ccgacaagca ctgccgcttt ctgcaccgcc
     2401 tgctcagtcg ccgcacgcgc ctgtacaccg agatggtgac caccggcgcc ctgctgcacg
     2461 gcgccgagcg gcgccacctg cgctttgacg ccgtcgagca cccgctggcg ctgcagctgg
     2521 gtggcagcga agcggccgat ctggccgcct gcgcccgcat cggtgcgcgc tggggctatg
     2581 acgagatcaa cctgaactgc ggctgcccca gcgaacgggt gcagcgcggc gccttcggtg
     2641 cctgcctgat ggccgagccg cagctggtgg ccgacggcgt caaggccatg gtcgatgccg
     2701 tcgacgtgcc ggtctccgtc aagcaccgca tcggcatcga ccgcgaggag agctacgact
     2761 tcgtgcgcga cttcgtcggc acggtggccg aggccggctg ccgcgtcttc atcgtgcatg
     2821 cgcgcaacgc ctggctgcag ggcctgagcc cgaaggagaa ccgcgaaatc ccgccgttgc
     2881 gccatgccct ggtgtatcgg ctcaagcgcg acttcccgca gctcaccatc gtgctcaacg
     2941 gcggcgtcac cagcgacgcg cagatcaccg aacatctgca gcacgtcgat ggcgtgatgc
     3001 tgggccgcga ggcctaccac cacccctgga tgatgagccg ctgggacgcc gaatttctgg
     3061 gcggtgccgc ctgcgcgctg acgcaggacg aggtcgaggc cgagatggtc gcctacatgg
     3121 cgcgcgaact ggccgaggac ggcacgccct ggtcggccat cgcgcgccac atgctgggcc
     3181 tgcaccatgg ccggcgcggc gcccgcctgt ggcgccaggt gtggagcgac caccgtctga
     3241 aggcacggcc ggcggccgag gtggcggcgc tggcccggtc gcatctggcg gccggggcac
     3301 acgcagggca gggcgccgtg gcgggcgcct gacggcacct ggctcgttcg cccttcgctg
     3361 tggcagccga ccctagaaac ggcagttgac cgctggtcaa cttcgggcag accgcatcga
     3421 tgcggggtct ggcggctgcg atggccttca cctcctgggt gagcacgctg cgcacccgcc
     3481 cgcaccgcgc tcggcgcgcc atgcggcgtt gcttcttctt gcgcgcagga gcccgcggcg
     3541 tcgtcgcgtc ttgcctggca cgccgatcac ggcacgggcg ggcgcgtcca actgccgtgt
     3601 ctgggccaag cgatggcagc gggctcgtgc ggcgcgctgc gatacagcat cctgttgatg
     3661 gtgtttcggg cttctggcgg ctgcccagac tgcgcaagca gctaccgtat tgatagcatc
     3721 gggttcccgg tcagcctggt gaccgttgca gcacccgcac cgtagtccag cgggcgctgg
     3781 ctctgcgtcg gcgcatcagc gatcggcccg ccagccctgc accagcgcgc ccagcgccat
     3841 gcccgccacc gcccacagca ggcttaagtt gccggcgccc aggccggcga tggccgggcc
     3901 ggggcacaca ccggtcagac cccagccgac gccgaacagc gccgcgccgc gcagtgtgtc
     3961 gcggttccag ctgctcgggt gcacgccgaa actgccgccc agcagcggcc gctgcagcag
     4021 gcgcggcagc agcttgtagg ccagcagcgt caccgcgacg ccgccgccca tcaccagcgc
     4081 caggcccagg tcgcgcccct gcaaaaacgc cagcaccacc tcgggccgga tcatggaaga
     4141 cagcgccagc ccgaagccga acagcgcgcc cgcggccagc gtggccagtc ccttggcgaa
     4201 gctcatacga tccacctcgt caccagattg gccgtgatga tggctgtgcc catgaaggtc
     4261 agcaccgcca gcgccgacgg ccactgcagc gaacccagcc cgcagatgcc atggcccgag
     4321 gtgcagccat tgcccagccg cgccccgtag cccgccacaa aaccgcccgc cagcagcagc
     4381 caccagggca cctgcgtgcc ttcggccacg ccggcgccgc gccacagcca ccaggccaaa
     4441 gcgccagcga tcagtcccag cgcatacagc agccgccagc cgcgcgtgtc caccagccgc
     4501 gcctgctgaa agaacggccg ccgcacgaca tacgaccagg tgctggaaaa caccgtgctc
     4561 atgccgccga tgcggccggt gaaaagaaac agcagcgcca cgccggcgcc cagacacagg
     4621 ccgcccagca ggtagtgctg ccaacccatg ggaaaaggcg tttgcaggat gctcatgcgg
     4681 tcatcgtagc aatccgttgc ggtagctgcc catggtgggg gcgccccgtg ccggagctgg
     4741 cagttttgaa ccggctgggc gcaggcttac agccaacgct gtcccggcgc cggcatggtc
     4801 tcgatggtga actcgggggt tccgcccagc accagaccca gcacgatgct gagcagcgac
     4861 atgaagatgc tggcccagaa cgccgtccag aaaccatcca gatgaaagcc gcgcaccagc
     4921 ctggccacca gcatcagcac cagcgcgttg atgaccagca ggaacaggcc gaagctgagc
     4981 agggtgagcg gcagcgtcag cagcaccagc aagggccgca ccaccgcatt ggccagcccc
     5041 agcagcaggg cggccaccac cagcgccccg ccgccgtcaa agcgcaggcc cttgaacacg
     5101 tgcgccgcca cccacagcgc gaaggcggtg atggcccagt gcagcaggaa gggggtgagg
     5161 ttggcgagca tgtcggcggt gtagcgatgc aggggtggat cgtgaccgtg gaaggtcggt
     5221 cagggtggcg ggggcgaagc gcggcagggc gctacttgcc ccacgagtcc ttcaggcccg
     5281 tcacccggtt gaacaccagc ctgtcacccg ccacatgctc ggcccgatcg gcgacgaaga
     5341 agccgtggcg ctcgaactga tagcggtctt ccggctgcgc gcccgccagc gacggctcga
     5401 cgatggcctg cacggtggtg agtgaccttg ggttgagcac gctgagaaag tcgcgcccgc
     5461 cggcgtcggg ctgttcttcg gtgaacaggc ggtcgtacag gcgcacttcg gccggcaggc
     5521 cgtcggccgc ggccacccag gtgatcacgc ccttgacctt gacgctgtcg gcgccagggg
     5581 tgccggattt ggtgtctttt atgagtctgg cgcttacccc gcttgcgcga ccagcttcat
     5641 ctttgatagc gcccaggcac tcgatcacgt agccgtattt gaggcgcacc ttgttgccgg
     5701 ggaacaggcg aaagaagccc ttgggcggcg tgtcctcgta gtcgctgcgc tcgatccaca
     5761 gttcgcgccc cagcatgaac tggcgctggc ccagctccgg gtgatgcggg tgcacggggg
     5821 cgtggcaggg ctccaggtgg tcggggctgc caaacacttc ggtccagttc tcgatcacca
     5881 gcttgagcgg ctccagcacc gccatggcgc gcggcgccac cgggtccagc gtttcacgca
     5941 gcgcggcgtc gagtgcggcg tagtcggtcc aggcgttgct ggtcttggtg acgcccgagc
     6001 gctcggcaaa caggcgcagg gcgtcgggcg tgtagccgcg ccgccgcagg ccggccagcg
     6061 tgggcatgcg cgggtcgtcc cagccggcca cgtggccttc ttccaccagc tggcgcagct
     6121 tgcgcttgct ggtgatgacg tggcccacgt tcaggcgcgc aaactcgtac tggcgcggtg
     6181 gcggggccgt gagcaggccg ccttcgcaca ggcgctcgag cagccagtcg tagaacgggc
     6241 gctggtcttc gaactccagc gtgcagatgc tgtgggtgat ttgctccagc gcgtcctcga
     6301 tcgggtgcgc gaaggtgtac atggggtaga tgcaccactt gtcgccggtg ttgtggtgcg
     6361 tggcgtggcg gatgcggtag atggccgggt cgcgcaggtt gatgttgggg ctggccatgt
     6421 cgatcttggc gcgcagcacc atggcgccgt ccgggtgctg gccatcgcgc atctcgcgaa
     6481 agcgcgccag gttctcggcc ggcgtgcggt ggcgaaacgg gctgtcggtg ccgggcgtgc
     6541 cgaagtcgcc gcggctggcg cggatctgct cggccgactg ctcgtccaca taggcgtggc
     6601 cggcttcgat caggtgctcg gcggcgcggt acatgaagtc gaagtagtcg ctggcaaagt
     6661 actcgtgcgg ctgtcgcacg ccaggtcgcg ccgggtcgtc ggcgaagtgg tggtcccagc
     6721 ccagccagcg caccatgtcg cggatggagt cgacgtattc ctgctcttcc ttctcggggt
     6781 tggtgtcgtc aaagcgcagg tggcacacgc cgtcgtattc ctgggccagg ctgaagttga
     6841 gccagatgct cttggcgtgg ccgatgtgca ggtagccgtt cggttcgggc ggaaagcggg
     6901 tgcggatcct ggccggatcg gccatgccgg cctggtggct ggggccgtcg cccggctggc
     6961 cgccccagcg gcgcagggcg taagtgccgg cctgcagatc gtgttcgatg gcctggcgga
     7021 tgaaatggct gggcttggcg gcctcggcgg ccgcagtgtc gttgggggaa ctcatggctt
     7081 cgattctagg aggccgcggg acgcgcccgc ccgatcggca gcgggcgccg gtggtgggcg
     7141 tctgcgggcc gctcgccaca ggggcctgaa cggggcctcg cggtcttttg ggttacgtat
     7201 cgatacccct gtcaccgcct ggcagtgcgc cggacggcgc gtcgctgcgt tacggtactg
     7261 ggcatcaaca tcggtcgccg tcacaggcgg catgagagga gttctgatga aatccctgca
     7321 aggcattctg atgacgggcc tgctggccgc gggcacgctg ggcgtgaccg gcgtggcgca
     7381 ggcgcgcagc gacgtgtact ggtcggtcgg cgtgggctca ccgggcgtgg ccattggcgt
     7441 gggcaacttg ccgccgccgg tgtatgtggc gccgcagccc gtgtacgtgg ccccgccgcc
     7501 ggtctacatg cgtccgcgcc cggtgtacgt ggccccgccg ccggcgtatt acgccccgcc
     7561 tcacgtggtg taccgcccgg cgccgcgcca ttactggggt gacggtcacc accattacaa
     7621 gcaccgcagg catcaccggc acgggcgcga ctgggactga cgccggtggc gcggcgccgc
     7681 ctttcgattc tccaagggca tctgtctggc acccgctccg tgcgagcggg tttttttgtc
     7741 gcacatccgc gcggcgcgag ctacaaggcg cggaaccggt gcgcgtagag ccggctcacc
     7801 accagccgtt cgccgcagcc gcgcaggctg aggtgcaggc gcccggcctc gtcgcgctgc
     7861 acgctgtcga tggcgtcggc acgcacggcc gtggaacgat ggatctgcca gaactcggcc
     7921 gggtcgagct gttccagcag gtctttcagc ggcgtgcgca gcagatgctc gccgctggcc
     7981 gtgaccacgc gcacgtattt gtcggccgcc tgcagcaaga gcacctcggc cacctgcacc
     8041 atgcggatgg tgttgccgcc gctgcccgcc gggctcacgg gcagctggcg cagccaagcg
     8101 gcgcgcggcg cggccggctg cagggcaggt tgcagggccg ccagcaggcg ctgcagctgc
     8161 gcggcgctgg cgtcggtcag ggcggatgct cctgattcag tagctgtccg cgcccgtcca
     8221 tcaagcgcca ggcgcagttt ttgcaccgat ttttccagcc gcgccggctg tacgggcttg
     8281 agcaggtagt ccatcgcctg cgcctcgaag gcctgcaccg cgtactggtc gtaggcggtg
     8341 acgaagacca gcgccggaaa cggcgccgcg gcctcgtccc aggcgtccat cagctcggcc
     8401 gcggcgtcca gcccgctctg gcccggcatg cggatgtcga agaacagcag gtcgggcttg
     8461 agcgccagcg cctcgcgcac ggcgctgatg ccatcgctca cgctcgcgac cacgcgcagc
     8521 tcgggccagg cgcgcgccag ctcggcctgc agggccgcgg ccagcagcgg ttcgtcctcg
     8581 gcgatcaggg cggtgagggc atgggtgctc atggtttcat cagcttccag agggcgggct
     8641 tgagcgtggc ggcggccatg aacccgccca tggccgcgcc ctcgatgccg agcgacgcca
     8701 cgtcctgccc cgcgagcagc aggccgggga cgggcgtgcg gacgtgcagc gcagggtcga
     8761 gcagtcgcgc gggcgtgcac gccaggccgt acatcgcccc ttcgggcgcc agcgcgtagg
     8821 cggcctgtgt cagcggggtg gagagctcgt ggtaggcaat gcggttcttc agcgccggga
     8881 acaggcgctt gaactggctg agcaggcggt cctcgatcca ctccttggtg gcctcgtagt
     8941 ccggcggtcg ctggcccagg ctggagtcgc gccaggtggc aaagaagcgc cagtcgcaca
     9001 gcgcgatcac ctcggccgtg ggcgcgtggg cctgctcggg atcgttcagg ccgccgaacg
     9061 atacgtacag cgccggggca tcggtatcgg tcgggcactg ccagagcaga gcctcggggt
     9121 cgggcgcgca gccaagcgcc tggccgtcgt agatccagtg gttggcgccg tcaaggccca
     9181 gcgcacgcaa ggcctcggcg ttgccctcca ggcccaggta gagcgtgatg taggcgtggc
     9241 tgggcaggtg ccggcgcagc gcgtcgcgcc agtcgggggc gagcccagcc ggcagcgcct
     9301 gcaccgtatt gagcgccccc atggccgaga cgacatggcg ggcacgctcg acaccgccct
     9361 ctgccagccg cacaccgacc acgcggccgc cctcggccag caactgcgtc acgcgcgcgc
     9421 cggtgcgcag cgcccctttg gctgcgcgca ccgcggccgc caggctgtcg gccagccgcg
     9481 ccgggccgcc gaccgggtag ccggcaccac cgtcggcgta gctgcccatg accagcgcgt
     9541 gcagcaccag cggcgcctgc gcgggcggca gaccatagtc gccaccacgc gcggccagca
     9601 gggcgcgcag gtcggcgccg tctatgccgg cgagcgcctg cccgagcgtc tcgcccgcac
     9661 ggcgccgcag ccacgggccc agcagagcgc gcaacaacgc ggcggcgggc cgcggcatgc
     9721 cgtgcagcgc catcaggatg ttgctgctgc ggcgcgcccg cgcgtagcgc gcggcgtacg
     9781 cgtcgatggc gccggcctcg gcgggatgct gcgccttcag ccgcgcccag ttgtcggcct
     9841 cgggggcacc gtaggcaaag cgcgtctcac cgccatcggc atgcaccagg cgaaccaggt
     9901 cgaattgacg gggcagtggc cgaaagcgca acgtgccgtc gccgaggacg ttcaacagcc
     9961 tggccaatcg cccctcgcca ccctcgggtg tcgtcacgcc cgtcaggtag tgcagaccgg
    10021 tggcgaagct gtagccgcga cgctcaaagg tctgcgtcat gccgcccagg ctgtcgtgct
    10081 gctccagcac cagcacgcgc tgcccgcagt gcgccagcgc cgcggcggcg gtcaggccac
    10141 cgatgcccga gccgatgacg atggtgtccc agttcatgac gtggcccctg acatcggcag
    10201 gcggatgcgc acggtggtgc cgccgccggg ccgcgattgc acatcgaccc gcgcacgccc
    10261 gccataggcg gtggcgatac gctcacgcac ctgcgccagg ccaaagcccc cggcacccgc
    10321 cgcaggctcc gtcatgtcaa agccgacgcc gctgtcgctt accgtcaaca ccagcgcatc
    10381 gcccgtgcgc cttgccgaca cctcgatgcg gccgccctcg atgcgcggct ccaggccatg
    10441 gcgaatggcg ttctccacga gtggctgcag cagcagcggc ggcaccggca gcgcggccag
    10501 cgcgtcgggc aggtccagcg cgacggccag gcgcgggccc atgcgcacgg ccatcagcgc
    10561 cagatagtcg tccaggcgcg cgaactcggc cgccagcggg tgctgcgcgc tgcgcgaggc
    10621 cgcgagcgtg gcgcgcaggt aggcgtcgag gcggtcgagc atctgctgcg ccgcctgcgg
    10681 atccacgccg atcagcgcgc gcagattggc cagggtgttg aacagcatgt gcggctcgag
    10741 ctggctttgc agcagcacca ggcgcgcctc gccggcgtcg cgctcggcgc gcgcgagctc
    10801 ggccgcgagc gccgcggcgc gtccgcgtgc atgaaagaaa aagctgccca cggcgcccgc
    10861 ggtgatggtg atcaacaggc cgagctggtc gtcgcgcgcc gagcgcaggt ccgcgccgag
    10921 aaacaggcgc cggcccaggg cctcgcccag cgtgaagccc gcggcgatgc cgatcgccgt
    10981 gagcagcagc gcgcgccagc ccctgggcca gccgtggccg ccctccaggc tggggtggca
    11041 gtgccgctca tcgaccgcgt agcgcccgaa ctctatggtg agccaggtca cgagcccaat
    11101 gcacagcgca tgacccagct gcgccgcgta gccatggccc ggccagatgc cggtggtgag
    11161 caccgcgatc acgcagcaga acgccacgac cagcagaaaa tgccggccca cgtcgcgcca
    11221 ggcgatgctc atgc
//
LOCUS       DAOOOG010000078        11206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6544_length_11206_cov_29.648552, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000078 DAOOOG010000000
VERSION     DAOOOG010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6544_length_11206_cov_29.648552"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..804
                     /gene="upp"
                     /locus_tag="PK242_07330"
     CDS             172..804
                     /gene="upp"
                     /locus_tag="PK242_07330"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOZ93865.1"
                     /translation="MPAEVHHVTHPLVQHKLTLMRNKDASSTTFRTLLGELAMLMAYE
                     VTRDTPTQLVEIETPLEKMMSPIIDGKKLVFASILRAGNGMLDGLLRVVPNARVGHVG
                     LYRDPATLQAVEYYFKMPTGMNERDVIVVDPMLATGHSAVAAIDRLKALAPRSIKFLC
                     LLAAPEGIRTLHASHPDVPIFTAAIDRELNDHGYILPGLGDAGDRIFGTK"
     gene            complement(982..2832)
                     /locus_tag="PK242_07335"
     CDS             complement(982..2832)
                     /locus_tag="PK242_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93866.1"
                     /translation="MSARAVDAVSALLAAALLAALLGLSVWLWQGGQRALLLAPAIGE
                     LADCLELAPAQTPLEPACSGERGSAAQRIEATLGPLGPRRSADGHFELGYTLVVPLLN
                     LFEPQGAGWAIDQQAVQRIVRTVRDVQRPVVLYLFSTHFSEQAPIEPVLAQDPANLAH
                     TPQGPLPVDQFMGWPLYPWSIARTDNAITQRREQAIGALVQGVCALPAAARQRIVGLN
                     LLGEVHHLYPDFEAGMGHDRPYVLTDYADASRRGFRAFLRQRFGHVAALNAYLGSDFA
                     SFDAVDPPSRDIRREPLQHFWQHLDDAAAGTLAISGWAHDAALPAGATPWVRVYLDGL
                     PVARVPAHFVRQDVGQALPQLGTDRVGWRHDLRFAELAPGLHRLDIALEGQGGALRRL
                     GTRRLAVMDRAQRTPAPVPLRQALPPMAEPGAALRFHIDTPADGRAVFYNPLVPLWHA
                     FRGQQVVDYLAHFDRLLAGTCLADVPRRTQQIYPAEKAGWDGSRFASEASLRPFGQVR
                     LGINLYGEASYDDSFFDWLARSRQSGYSVTEFHPLRGMDGAELRRVLEQHRAHGAETF
                     SFFLHPPPPGGTRSQPLANPFALDPQNPRHGSDTLYRALQQVMQAPGTGR"
     gene            complement(3013..3552)
                     /gene="msrA"
                     /locus_tag="PK242_07340"
     CDS             complement(3013..3552)
                     /gene="msrA"
                     /locus_tag="PK242_07340"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOZ93867.1"
                     /translation="MSDSQTLVLAGGCFWCTEAVFKQVRGVLDVESGYSNGQATAPSY
                     EQVCTGATGCAEVVKLVYDPGQIGTRQILEIFFLVHDPTQLNRQGHDVGTQYRSGVYF
                     TTAEQADVAHALIREMSQDGLYDGAIVTEVAPLAHYFPAEDYHQDFFERNPTQGYCVA
                     TAAPKVAKFRQTFRELVKA"
     gene            3866..4921
                     /locus_tag="PK242_07345"
     CDS             3866..4921
                     /locus_tag="PK242_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOZ93868.1"
                     /translation="MNAMPHPPTPRRWRAARLVPALCAGLLAGLAPPAQAAWEPTAPV
                     ELVVPAGTGGGADQMARFIQQMVSKHGLMKQPLNVVNKTGNSGVEGLMDVKAAQGNPH
                     KLIITLSNLFTAPLATGTPFAWSDITPVQMLALDEFVLWVAKGSPYKSPKDVLDALRA
                     SPAGSLRLGGTGTKQEDQLISVLLETAASARIQYVPFKGGGDVARALAAGEVDLTVNN
                     PIEAEKLWREGKLRPLCVFDGSKLAYPDKVADNQSWSDLPTCMSFGIPVEYLMMRGIF
                     MAPGATPEQVAYYAQLLDKVRALPEWNALMKQGAYKQTTMAGPSFSSWLDRSNRFHRT
                     LMREARLTAAAPGAARN"
     gene            complement(4933..5415)
                     /locus_tag="PK242_07350"
     CDS             complement(4933..5415)
                     /locus_tag="PK242_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOZ93869.1"
                     /translation="MTTPRQYWLMKSEPDECSIDDALAAPGQTVPWTGVRNHQARNFM
                     RDAMRVGDGVLFYHSSCAEPGIAGIARVASAAHADPTQFDPGSAYFDPTARPDSPRWL
                     LVDVQALRKTRLLGLAELRGVPALADMLVLRRGNRLSITPVTPGQWQAIERLLAAAPA
                     "
     gene            5447..6496
                     /locus_tag="PK242_07355"
     CDS             5447..6496
                     /locus_tag="PK242_07355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOZ93870.1"
                     /translation="MTDHAHPFATLTPDFVLDALAALGLMGDGRLMTLNSYENRVYLA
                     HLEPGTPLAEAHPAVVLKFYRPGRWTQAQIEEEHAFARELAAAEVPVVAPLVIDGRTL
                     HRHGDFWFSLTPRRGGRAPELGDADTLAWMGRFLARLHLVGARQAFVHRPALDLRSHG
                     VEPREWLLAHAALPPEAERAWAEVSQDAIDHIAAGADWASSETHFDHPTRPIATLRLH
                     ADCHVGNILWTPTEQPGGGPHFVDLDDCRSGPAVQDLWMLLAGERAERQRQLGALLDG
                     YEQVRAFDRRELALIEPLRTLRLIHYSAWLAQRAHDPAFAAAFAGFGTPDYWRGQTAL
                     LREQIEAMAEPPLCA"
     gene            6603..7262
                     /locus_tag="PK242_07360"
     CDS             6603..7262
                     /locus_tag="PK242_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ93871.1"
                     /translation="MNTLQDLPCPPSRRTRRKQARPGELLDAALALFVEKGFAATRVE
                     EVATRAGVSKGTLFLYFPSKQALFKAVVRENVGRHLDQALADLASYTGSSADLLREFL
                     RRWWTHYGNTPAAGLCKLIVVEVAHFPELARDYQEQVVLPSHELLRRVLERGIASGEF
                     RRVDVAQTVPLIIAPLLQLVLWRQWPADCMPAPTSDPQALLAQHADLLLHGLRPDTAT
                     P"
     gene            7259..8434
                     /locus_tag="PK242_07365"
     CDS             7259..8434
                     /locus_tag="PK242_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ93872.1"
                     /translation="MNPSFSRGLVRALLLLAVLAAVLLGWRGYQARQAKAPAPAAAAA
                     SAPVIELAATDLAHARQVALGTDIPITGTLKAVRSALVKARVPGELQGLTVREGDRVR
                     AGQVLARIEPTEYSERLRQTQQQADAARAQVDIAQRQYDNNAALVNQGFISRTALDNS
                     LASLEAARANHRAAQAGVEVVRKSLADTVLRAPINGQIAQRLAQPGERVATEARIVEI
                     VDASELELEAALSPADSVAVRVGQSAHLDIEGVTQPVAARVVRINPSAQPGSRSVLVY
                     LSVAPQPGLRQGLFAQGQLATGEQQALAVPLSAVRTDKPAPYVQVVAGERIAHRPVRL
                     GVRGRVDGEPWVQVEGVDAGATVLRGSAGVLREGTATRVPTPPAPASSAPAEAARSW"
     gene            8590..>11206
                     /locus_tag="PK242_07370"
     CDS             8590..>11206
                     /locus_tag="PK242_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ93873.1"
                     /translation="MWFTKVSLNNPVFAAMVMLAFVVLGLFSLQRLQVDQFPNVDFPV
                     VVITTEYPGAAPTIVESEVTKKVEEAVNSVAGINALYSRSYENLSVVIVEFQLHIEGR
                     KAAEDVREKIGQVRPLLREEVKEPRVLRFDPASRAIWSVAVLPQASEGRAAPSPIELT
                     NWADQVLKKRLENVRGVGAVNLVGGAKREIHIQLRPAALEALGVTPEQVMAAVRGDNQ
                     DQPLGALRTPAQELTVQLQGRVERPEDFGRLIVARRGGAPIHLAQVADVRDGAQEQES
                     LALHNGQRTLLLNVQKAQDENTIGVVDGLYRAVAEIRAELPPGVRLEPVIDQSRPIRV
                     AVQNVRQTLIEGAVLTVLIVFLFLNSWRSTVITGLTLPISIIGTFFFMQVLGFTVNMI
                     TLMALSLCVGLLIDDAIVVRENIVRHAQMGKRSYDAAMDGTQEIGLAVLATTLSIVAV
                     FLPIGFMQGIIGKFFHEFGLTIVAAVLISMFVSFTLDPMLSSVWHDPSIHAHGQRSGP
                     PVTLYERTIGRVTAWFDHASDRLADGYQRLLGWSLAHKPLTLLIAAAIFGLSVAMVRL
                     LGTEFVPKADFSETNVAFYTPQGSSLEATEAKARQVDALLRSYPEVRYTVATLNAGNA
                     QGKTNASLYIRLVDRHERTRNADDLAADFRQRLRAVPGITVTQAGLLEPVGGQKQIEF
                     SLQGPDQAELERLARPLMERLRDIPGLVDLDSSSKPDKPTVEVTVKRAAASELGLSSA
                     QIAAPLRTLIAGTTVGNWRAPDDQTYDVVVRLAADARSVPQDLERLPLTAGLNADGTP
                     RIVRLNQVATLNEASGTNQINRRAMLREIQITANTHGRATGEVSGDIRAALAQMPFPP
                     GYSYSFGGATKNMEES"
BASE COUNT         1722 a         4046 c         3889 g         1549 t
ORIGIN      
        1 acgacggcct ggtgcagaac gtggtcaagg gcgagatgcg ccgcatgggc cgcgaactgc
       61 tgcgcggcgc cctgggcagc ctgctgggcg gccgccggcg ttgagcacct gaaccgcccc
      121 caacgcttta ccatcctgcg ccagcagcta ccttttcaat agcgattgac gatgcccgcc
      181 gaagtccacc acgtcaccca cccgctggtg cagcacaagc tgaccctgat gcgcaacaag
      241 gatgccagca gcaccacctt ccgcaccctg ctgggtgagc tggcgatgct gatggcctac
      301 gaggtcacgc gcgacacgcc gacccagctg gtcgagatcg agaccccgct ggagaagatg
      361 atgagcccca tcatcgacgg caagaaactg gtgttcgcct ccatcctgcg cgccggcaac
      421 ggcatgctgg acggcctgct gcgcgtggtg cccaacgccc gcgtcggcca cgtcggcctg
      481 taccgcgacc cggccacgct gcaggcggtg gagtactact tcaagatgcc caccggcatg
      541 aacgagcgcg acgtgatcgt ggtcgacccg atgctggcca ccggccattc ggcggtggcg
      601 gcgatcgacc gcctcaaggc gctggcgccg cgctcgatca agttcctgtg cctgctggcc
      661 gcgcccgagg gcatccgcac cctgcacgcc agccacccgg acgtgccgat cttcactgcc
      721 gccatcgacc gcgagctcaa cgatcacggc tacatcctgc cgggactggg ggatgcggga
      781 gatcgcatct tcggcacgaa gtgactcccc cctgaggggc gggcgccgct ggtggccgag
      841 gggaccccgg ccacggcggc ctggcttgac ctgctccgcg gcccttattg gggtgtgctg
      901 cgccgcccct ttggatgcgg cggatactcc gtttttaata gctgttcgcg atgatctgtc
      961 aagcgctggc ggccgatttg tttaacgtcc tgtgcctggg gcctgcatca cctgctgcag
     1021 cgcgcggtac agggtgtcgg agccgtggcg cgggttctgg gggtccaacg caaaggggtt
     1081 ggccagcggt tgcgagcggg tgccgccggg cggcggcggg tgcaggaaga aggagaaggt
     1141 ttcggcgccg tgcgcgcggt gctgctccag cacgcggcgc agctcggcgc cgtccatgcc
     1201 gcgcagcggg tggaactcgg tcacgctgta gccgctctgg cgcgaacgcg ccagccagtc
     1261 gaagaaggaa tcgtcgtagc tggcctcgcc atacaggttg atgcccaggc gcacctgacc
     1321 gaacggccgc agcgaggcct cggacgcaaa gcgcgagccg tcccagccgg ctttttcggc
     1381 cgggtagatc tgctgcgtgc ggcgcggcac gtcggccagg caggtgccgg ccagcaggcg
     1441 atcgaaatgc gccaggtagt ccaccacctg ctggccgcgg aaggcgtgcc acaacggcac
     1501 cagcgggttg tagaacacgg cacgcccatc cgccggcgtg tcgatgtgaa agcgcagcgc
     1561 ggcgcccggc tcggccatgg gcggcagcgc ctgacgcagc ggcaccggcg ccggcgtgcg
     1621 ctgggcgcgg tccatcacgg cgagccggcg cgtgcccagc cggcgcagcg cgccgccctg
     1681 gccttccagc gcgatgtcca gccggtgcag gccgggggcg agttcggcaa agcgcaggtc
     1741 atgccgccag ccgacacggt cggtgcccag ctgtggcagc gcctggccga cgtcctggcg
     1801 cacgaaatgc gccggcacgc gcgccaccgg cagaccgtcc aggtacacgc gcacccaggg
     1861 cgtggcgccg gccggcagcg cggcgtcgtg cgcccagccg ctgatggcca gcgtgccggc
     1921 ggcggcatcg tccaggtgct gccagaagtg ctgcagcggc tcgcggcgga tgtcgcgcga
     1981 aggcggatcg acggcgtcga agctggcgaa gtccgacccc aggtaggcgt tgagcgccgc
     2041 cacgtggccg aagcgctggc gcagaaaggc gcgaaagccg cggcgcgagg cgtcggcgta
     2101 gtcggtcagc acgtagggcc ggtcgtggcc catgccggcc tcgaagtcgg ggtacaggtg
     2161 gtgcacctcg cccagcaggt tcaggccgac gatgcgctgg cgcgccgccg ccggcagcgc
     2221 gcacacgccc tgcaccagcg cgccgatggc ctgctcgcgc cgctgggtga tggcgttgtc
     2281 ggtgcgggcg atgctccagg ggtacagcgg ccagcccatg aactggtcca ccggcagcgg
     2341 gccctgcggc gtgtgcgcca ggttggccgg gtcctgcgcc agcaccggct cgatcggcgc
     2401 ctgctcggaa aaatgggtgg aaaacaggta cagcaccacc ggccgctgca cgtcgcgcac
     2461 ggtgcggacg atgcgctgca ccgcctgctg gtcgatggcc cagccggcgc cctgcggctc
     2521 gaacaggttg agcagcggca ccaccagggt gtagccgagc tcgaagtgcc cgtcggcact
     2581 gcggcgcggg cccagcgggc cgagcgtggc ctcgatgcgc tgcgcggccg agccgcgctc
     2641 gccgctgcag gcgggctcca gcggcgtctg cgccggcgcc agttccagac agtcggccag
     2701 ctcgccgatg gccggtgcca gcagcagcgc gcgctgcccg ccctgccaca gccagaccga
     2761 caggcccagc agcgcggcca gcagcgcggc ggccagcagc gcgctcaccg cgtcgacggc
     2821 gcgcgcgctc atcggccgca cccgcacgag cacgcctcgg gctgggcggc agcacaccga
     2881 ccagcctggc gcgcggccgg gtcggccggc atcaccgttg ggcagaaaac tatcgaattc
     2941 atagctgctc gcgcagattc agcaagcggc gcaggccaat gggttgccca tcggatgctt
     3001 gccggacggg cctcaggcct tcaccagctc gcggaaagtc tggcgaaact tggccacctt
     3061 gggcgccgcc gtggccacgc agtagccctg ggtcgggttg cgctcgaaaa aatcttgatg
     3121 gtagtcctcg gccgggaagt agtgggccag tggcgccacc tcggtcacga tggcgccgtc
     3181 gtacagcccg tcctggctca tctcgcgaat cagcgcgtgc gccacgtcgg cctgctcggc
     3241 ggtggtgaag tacacgccgc tgcggtactg ggtgcccaca tcgtggccct ggcggttgag
     3301 ctgggtcggg tcgtgcacca gaaagaagat ctccaggatc tggcgcgtgc cgatctgccc
     3361 cgggtcgtac accagcttga ccacctcggc gcaaccggtg gcgccggtgc acacctgctc
     3421 gtagctgggc gccgtggcct ggccattgct gtagccgctt tcgacgtcga gcacgccgcg
     3481 cacctgcttg aacaccgcct cggtgcacca gaagcagccg ccggccagca ccagggtttg
     3541 actgtcagac atccatccac tccttcgatc gggccgcacc aggccccgtg tcgcccacga
     3601 gcaaccaccg cccgcacccg gccccggcgt gcattgtgct ccgccgccgg ccagccccag
     3661 cccgcaccca tgcgacgcgc cctggcggca gaccggcgac cccgccggcg gcgcccgacg
     3721 cccaagcccg gggcgtcgtg atttagtaat caataatgtc taaatatttc cggcaacggc
     3781 gtttcaggac cggccctgtc ccctcagaat caggccgcac cggcaacacc tcatcactcg
     3841 ccgaacgcat cgatacccgc agaccatgaa cgccatgcca caccccccca ccccgcggcg
     3901 ctggcgcgcc gctcggcttg tacccgccct ctgcgctggc ctgctggccg gcctggcgcc
     3961 accggcgcag gcggcctggg aaccgacggc accggtggag ctggtggtgc ccgccggcac
     4021 cggcggcggt gccgaccaga tggcccgctt catccagcag atggtgagca agcatggcct
     4081 gatgaagcag ccgctgaacg tggtcaacaa gacgggcaat tcgggggtcg agggcctgat
     4141 ggacgtcaaa gcggcgcagg gcaacccgca caagctcatc atcacgctgt ccaacctgtt
     4201 caccgcgccg ctggccaccg gcacgccgtt tgcctggtcg gacatcaccc cggtgcagat
     4261 gctggcgctc gacgagttcg tgctgtgggt agccaagggc tcgccgtaca agtcgcccaa
     4321 ggacgtgctg gatgcgctgc gcgccagccc ggccggcagc ctcaggctcg gcggcaccgg
     4381 caccaagcag gaagaccagc tgatcagcgt gctgctggaa accgcggcca gcgcgcgcat
     4441 ccagtacgtg ccgttcaagg gcggcggcga cgtggcacgg gcactggccg ccggtgaggt
     4501 ggacctgacg gtcaacaacc cgatcgaggc cgaaaagctg tggcgcgaag gcaagctgcg
     4561 gccgctgtgc gtgttcgacg gcagcaaact ggcctatccc gacaaggtgg ccgacaacca
     4621 gagctggagc gacctgccca cctgcatgtc gttcggcatc ccggtggaat acctgatgat
     4681 gcgcggcatc ttcatggcgc cgggtgccac gcccgagcag gtggcgtact acgcgcagct
     4741 gctcgacaag gtgcgcgcac tgcccgaatg gaacgctctg atgaagcagg gcgcctacaa
     4801 gcagaccacc atggccgggc cgagcttcag cagctggctc gatcgcagca accgctttca
     4861 ccgcaccttg atgcgcgaag ccaggttgac ggccgccgcc cccggcgccg ccaggaactg
     4921 aggcattgcc ggtcacgccg gcgcggcggc cagcaggcgc tcgatcgcct gccactgacc
     4981 cggcgtcacc ggtgtgatcg acagccggtt gccgcgccgc agcaccagca tgtcggccag
     5041 cgccggcacg ccgcgcagct cggccaggcc cagcaggcgc gtcttgcgca gcgcctgcac
     5101 gtccaccagc agccagcgcg ggctgtcggg ccgggcggtg gggtcgaagt acgcggagcc
     5161 ggggtcgaac tgggtcgggt cggcatgggc ggcggaggcc acgcgcgcaa tgccggcgat
     5221 gcccggctcg gcacagctgg agtggtagaa cagcacgccg tcgcccaccc gcatggcgtc
     5281 gcgcatgaag ttgcgcgcct ggtggttgcg caccccggtc cacggcacgg tctggccagg
     5341 cgccgccagc gcgtcgtcga tcgagcactc gtcgggttcg gatttcatca accagtactg
     5401 gcgcggcgtc gtcatggcgc cgattgtgcc gccgacaatg ctgggcatga ccgaccacgc
     5461 ccaccccttc gccaccctca cccccgactt cgtgctggac gcgctggccg ccctggggct
     5521 gatgggcgat gggcgactga tgacgctcaa ctcgtacgag aaccgcgtct acctggcgca
     5581 tctggagccg ggcacgccgc tggccgaggc gcacccggcg gtggtgctca agttctaccg
     5641 ccccgggcgc tggacgcagg cgcagatcga ggaagagcac gccttcgcgc gggagctggc
     5701 cgcggccgag gtgccggtgg tggcgcccct ggtgatcgac gggcgcacgc tgcaccggca
     5761 cggcgacttc tggttcagcc tgacgccacg gcgcggcggc cgcgcgcccg agctgggcga
     5821 cgccgacacg ctggcatgga tgggccgctt tctggcccgg ctgcacctgg tgggggcgcg
     5881 ccaggcgttt gtgcaccgcc ccgcgctcga cctgcgcagc cacggcgtgg agccgcgtga
     5941 atggctgctg gcgcacgctg cgctgccgcc ggaggccgag cgcgcatggg ccgaagtcag
     6001 ccaggatgct atcgatcaca tagctgctgg cgcagattgg gcaagctctg aaacccattt
     6061 tgaccatcca acccggccga tcgccacgct gcgtctgcac gccgactgcc atgtcggcaa
     6121 catcctgtgg acgccgaccg agcagcccgg cggcggcccg cacttcgtcg atctggacga
     6181 ctgccgcagt ggcccggcgg tgcaggacct gtggatgctg ctggccggcg agcgcgccga
     6241 gcgccagcgc cagctgggcg cgctgctgga cggctacgag caggtgcgcg cgttcgaccg
     6301 gcgcgagctg gcgctgatcg agccgctgcg cacgctgcga ctgatccact acagcgcctg
     6361 gctggcgcag cgcgcgcacg acccggcctt tgccgcagcc tttgccggct tcggcacgcc
     6421 cgactactgg cgcggccaga cggcgctgct gcgcgagcag atcgaggcca tggccgagcc
     6481 gccgctgtgc gcctgagcgc ctgcgcgcgc cgccgcgcca gcggacaacg gcgcggcccc
     6541 gcccgctcga tcttgacgcc gttcaggcgc ctgcgccaaa atactgaccc cccagtcatt
     6601 aaatgaacac cctccaggac ctcccgtgcc cgccgtcccg gcgcacgcgc cgcaagcagg
     6661 cgcggccggg cgagctgctg gacgccgcgc tggcgctgtt cgtcgaaaag ggctttgccg
     6721 ccacccgggt ggaagaagtc gccacccgcg ccggcgtctc caagggcacg ctgtttctgt
     6781 actttcccag caagcaggcg ctgttcaagg ccgtggtgcg cgaaaacgtc ggccgccacc
     6841 tggaccaggc gctggccgat ctggccagct acaccggctc cagcgccgat ctgctgcgcg
     6901 aattcctgcg ccgctggtgg acccattacg gcaacacgcc cgccgccggg ctgtgcaagc
     6961 tgatcgtggt cgaggtggcc cactttcccg aactggcgcg cgactaccag gagcaggtgg
     7021 tgctgcccag ccatgagctg ctgcgccgcg tgctggagcg cggcatcgcc agtggcgaat
     7081 tccgccgcgt cgatgtggcg cagacggtgc cgctcatcat cgcccccctg ctgcagctgg
     7141 tgctgtggcg ccagtggccg gccgactgca tgccggcgcc aacctccgat ccccaggccc
     7201 tgctggcgca gcacgccgac ctgctgctgc acggcttgcg ccccgacacc gcaacaccat
     7261 gaatccgtcc ttctcccgtg ggctcgtccg ggccttgttg ctgctggccg tgctggccgc
     7321 cgtgctgctg ggctggcgcg gctaccaggc ccggcaggcc aaggccccgg cgccggctgc
     7381 ggccgcggcc agcgcgccgg tgatcgaact ggccgcgacc gacctggcgc acgcacgcca
     7441 ggtggcgctg ggcacggaca tccccatcac cggcacgctc aaggcggtgc gctcggccct
     7501 ggtcaaggcg cgcgtgccgg gcgagctgca agggctgacg gtgcgcgaag gcgaccgcgt
     7561 gcgcgccggc caggtgctgg cccgcatcga gccgaccgag tacagcgagc gcctgcgcca
     7621 gacccagcag caggccgacg ccgcgcgcgc ccaggtggac atcgcccagc gccagtacga
     7681 caacaacgcc gcgctggtca accagggctt catctcgcgc accgcgctgg acaactcgct
     7741 ggccagcctg gaagcggcgc gcgccaacca ccgcgcggcg caggcgggcg tggaagtggt
     7801 gcgcaagtcg ctggccgaca ccgtgctgcg cgcgccgatc aacggccaga tcgcgcagcg
     7861 cctggcccag ccgggcgaac gcgtggcgac cgaggcgcgc atcgtcgaaa tcgtcgacgc
     7921 cagcgagctg gagctggagg ccgcgctcag cccggccgat tcggtggcgg tgcgggtggg
     7981 ccagagcgcg cacctggaca tcgagggcgt gacccagccg gtggccgcgc gcgtggtgcg
     8041 catcaacccc agcgcccaac cgggcagccg cagcgtgctg gtgtacctga gcgtggcgcc
     8101 ccagcccggc ctgcgccagg gcctgttcgc ccagggccag ctggccaccg gcgagcagca
     8161 ggcgctggcc gtgcccctga gcgccgtgcg caccgacaaa ccggcgccct acgtgcaggt
     8221 ggtggcgggc gagcgcatcg cccaccggcc ggtgcgcctg ggcgtacgcg gccgcgtgga
     8281 cggcgagccg tgggtgcagg tcgaaggcgt ggacgccggc gccaccgtgc tgcgcggcag
     8341 cgccggcgtg ctgcgcgaag gcacggccac ccgtgtgccg acgccgcccg ccccggccag
     8401 cagcgccccc gccgaggccg cgcgctcgtg gtgacccgcg cccgcatgcc cgccagcgcc
     8461 gcccgggtgg caccacccag cggccagata gctacctttt tgatagctgc tggcgcgcat
     8521 tccgcaagcg ccaaaggcat tttctacatg tattcatcgg gccgccgcgc ctgagcctcg
     8581 caccccgcca tgtggttcac caaagtcagc ctcaacaacc cggtgttcgc cgccatggtg
     8641 atgctggcct tcgtggtgct gggcctgttt tcgctgcagc ggctgcaggt cgatcagttc
     8701 cccaacgtcg acttcccggt ggtggtcatc accaccgaat acccgggtgc ggcgcccacc
     8761 atcgtcgaga gcgaagtcac caagaaggtc gaggaagcgg tcaactcggt ggccggcatc
     8821 aacgccctgt actcgcgcag ctacgagaac ctgtcggtgg tgatcgtgga gttccagctg
     8881 cacatcgagg gccgcaaggc ggccgaggac gtgcgcgaaa aaatcggcca ggtgcgcccg
     8941 ctgctgcgcg aagaggtcaa ggagccgcgc gtgctgcgtt tcgacccggc cagccgcgcc
     9001 atctggtcgg tggcggtgct gccgcaggcc agcgagggtc gggccgcgcc ctcgcccatc
     9061 gagctgacca actgggccga tcaggtgctg aagaagcgcc tggaaaacgt gcgcggcgtc
     9121 ggtgccgtca acctggtggg cggtgccaag cgtgaaatcc acatccagct gcgaccggcc
     9181 gcgctggaag ccctgggcgt gacgcccgag caggtgatgg ccgccgtgcg cggcgacaac
     9241 caggaccagc cgctgggcgc gctgcgcacc cccgcgcagg agctgacggt gcagctgcag
     9301 ggccgggtgg agcggcccga ggacttcggc cgcctgatcg tggcgcggcg cggcggcgcg
     9361 ccgatccacc tggcgcaggt ggccgacgtg cgcgacggcg cgcaggagca ggaatcgctg
     9421 gccctgcaca acggccagcg caccctgctg ctgaacgtgc agaaggcgca ggacgagaac
     9481 accatcggcg tggtcgacgg gctgtaccgc gcggtggccg agatccgcgc cgagctgccg
     9541 cccggcgtgc ggctggagcc ggtgatcgac cagtcgcgcc cgatccgcgt ggcggtgcag
     9601 aacgtgcgcc agacgctgat cgagggcgcc gtgctgacgg tgctgatcgt gttcctgttc
     9661 ctcaactcct ggcgcagcac cgtcatcacc ggtctgacgc tgccgatctc catcatcggc
     9721 actttcttct tcatgcaggt gctgggcttc accgtcaaca tgatcacgct gatggcgctg
     9781 tcgctgtgcg tcggcctgct gatcgacgac gccatcgtgg tgcgcgagaa catcgtgcgc
     9841 cacgcccaga tgggcaagcg ctcgtacgac gccgccatgg acggcacgca ggaaatcggc
     9901 ctggcggtgc tggccaccac gctgtcgatc gtggcggtgt tcctgccgat cggcttcatg
     9961 cagggcatca tcggcaagtt cttccacgag ttcggcctga ccatcgtggc ggcggtgctg
    10021 atctcgatgt tcgtcagctt cacgctcgac cccatgctgt cgtcggtctg gcacgacccc
    10081 agcatccatg cccacgggca gcgcagcgga ccgccggtga cgctgtacga acgcaccatc
    10141 ggccgcgtca ccgcctggtt cgaccacgcc agcgaccgcc tggccgacgg ctaccagcgc
    10201 ctgctgggct ggtcgctggc gcacaagccg ctcacgctgc tgatcgccgc cgccatcttc
    10261 ggcctcagcg tggccatggt gcggctgctg ggcaccgaat tcgtgcccaa ggccgacttc
    10321 tccgaaacca acgtcgcctt ctacacccct cagggttcgt cgctcgaggc caccgaggcc
    10381 aaggcgcgcc aggtcgacgc cctgctgcgc agctaccccg aggtgcgcta caccgtcgcc
    10441 accctgaacg ccggcaacgc ccagggcaag accaacgcca gcctgtacat ccgcctggtc
    10501 gaccggcacg aacgcacgcg caacgccgac gatctggcgg ccgacttccg ccagcgcctg
    10561 cgcgccgtgc ccggcatcac cgtcacccag gccggcctgc tggagccggt gggcgggcaa
    10621 aagcagatcg agttctcgct gcagggcccg gaccaggccg agctcgaacg cctggcgcgc
    10681 ccgctgatgg agcgcctgcg cgacatcccc ggcctggtcg acctggactc cagcagcaag
    10741 cccgacaagc cgacggtgga agtgacggtc aagcgcgcgg ccgcctccga gctgggcctg
    10801 tccagcgcgc agatcgcggc gccgctgcgc accctgatcg ccggcaccac ggtgggcaac
    10861 tggcgcgcgc ccgacgacca gacctacgac gtggtggtgc ggctggcggc cgacgcccgc
    10921 agcgtgccgc aggacctgga gcgcctgccg ctgaccgccg gcctcaacgc cgacggcacg
    10981 ccgcgcatcg tgcgcctgaa ccaggtggcg acgctcaacg aagccagcgg caccaaccag
    11041 atcaaccgtc gcgccatgct gcgcgaaatc cagatcaccg ccaacacgca cggccgcgcc
    11101 accggcgagg tgtcaggcga catccgcgcc gcgctggcgc agatgccctt tccgcccggc
    11161 tacagctact cgttcggcgg cgccaccaag aacatggagg agtcct
//
LOCUS       DAOOOG010000079        11110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6613_length_11110_cov_24.020624, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000079 DAOOOG010000000
VERSION     DAOOOG010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6613_length_11110_cov_24.020624"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /gene="moaA"
                     /locus_tag="PK242_07375"
     CDS             <1..1002
                     /gene="moaA"
                     /locus_tag="PK242_07375"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOZ93874.1"
                     /translation="VTDRCNFRCTYCMPKEVFGADYPYLPHASLLTFEEITRLARLFI
                     AHGVRKIRLTGGEPLLRKNVEGLIEQLAALRTPDGEPLDITLTTNGSLLARKARALAA
                     AGLRRVTVSLDSLDDAVFQRMNDVGFPVREVLAGIEAAQAAGLAPIKVNMVVKRGTNA
                     HEIVPMARHFRGSGMALRFIEYMDVGATNGWRMDEVLPSAEVLRRLQAEFALVPLAHS
                     APGETAERWGHAGADGRHDPALGEIGLISSVTRPFCGDCNRARLSTEGRLYLCLFASQ
                     GYDLRALLRGGASDEAIGHAIGAIWGHRADRYSELRASGRLDPHSGERRIEMSYIGG"
     gene            1036..1722
                     /gene="mobA"
                     /locus_tag="PK242_07380"
     CDS             1036..1722
                     /gene="mobA"
                     /locus_tag="PK242_07380"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HOZ93875.1"
                     /translation="MTAIPGAPADPDRIPITALLLAGGRGSRMGGLDKGLQSFGGLPL
                     AQHALERLRRQTLPPAAMLINANRHLPDYQAFGVPVWPDVLPDYAGPLAGFLTGLAHA
                     QTPWMLTLPCDVPRFPLSLCERLAQALLAQKADIAMVAAPEEDGRLRPQPVFCLLRRE
                     LHDSLARFMAAGGRKIDAWTAQHRQVLVPFDHPGDDPRAFANANTLAELRQLEREPRQ
                     DACQPPMPSF"
     gene            1739..2260
                     /locus_tag="PK242_07385"
     CDS             1739..2260
                     /locus_tag="PK242_07385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOZ93876.1"
                     /translation="MPLPPLTIRLLGPADLPAYKALRDIGLRHDPEAFTTDFNAGSAL
                     PAAAYATRLGQPPEDHFILGAFDPAGAMLGAVVCERESRLKKRHEAALVGMIIAPEVR
                     SHGIGKALLAAFDQLVRQLPGLEQVVLSVTASNARAVALYEHAGFVRYGLLPRAIKID
                     GRYHDKALMVKVL"
     gene            2257..3627
                     /locus_tag="PK242_07390"
     CDS             2257..3627
                     /locus_tag="PK242_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOZ93877.1"
                     /translation="MTPTTAATTPRTADIAAALQGYDPQALHADAVTAFLQRLVEPVT
                     EVQRVPLPEALGRVLAEDVISPISVPPHDNSAMDGFAFDGAQLQPGQPLRLRCVGTAL
                     AGTAWRAAVAAGECVKIMTGAVMPAGLDTVVPQEFTRVEGAQIELPAGVVQRGDNRRQ
                     AGEDLMAGRPALRQGERLTPAALGLLASLGLGQVTLRRRLRVAYFSTGDEILSLGQPP
                     REGAVYDSNRYTVGGLLARLGVEVIDLGVVPDQPAALRAAFGQAAAQADAVITSGGVS
                     VGEADHTKAMMRELGDVVFWRIAMRPGRPMAVGRIVTPAPELQVKSASSANTANANSY
                     QNNSNSAILFGLPGNPVAVMVTFLAFVRPALLRMMGARPEPPVWLRARSSQAIAKKPG
                     RTEYQRGVVSTQPDGQLTVRTTGNQGSGVLSSMVQANGLIVLGHAQGDVAVGDEVRVM
                     MFEGAL"
     gene            complement(3646..4458)
                     /locus_tag="PK242_07395"
     CDS             complement(3646..4458)
                     /locus_tag="PK242_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ93878.1"
                     /translation="MFERILVPLDGSVFSEEMLAHATGLAAVRGTPLTLMRVVDKGAD
                     EAEARAYVEALAARHGARGICVVAHGDVADTVLDEAAGVPHTLVAMSSRGRSGLMEAV
                     LGSVAQRVVRGADAPVLVYRPTGRSAAGGGAVAVRRIVLPLDGSPLSEVMGLQAAEFA
                     RWAGAELEVVRVVDATGAAGLPAGGELAALESGYVRSKATELGRSHGVPVNWDVLHGD
                     AVQAIASHVAVRSDTVLAMVTRRQGAVETALLGSVTAGCLRQAGVPVLMRAP"
     gene            complement(4541..6874)
                     /locus_tag="PK242_07400"
     CDS             complement(4541..6874)
                     /locus_tag="PK242_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450/oxidoreductase"
                     /protein_id="HOZ93879.1"
                     /translation="MSETHPAAGACPVDHAALRDPNGRPVSAGAAAFDPFGDGYQQNP
                     PEYLRWARAQEPVFFAPQIGYWVVTRYDDIKAVFRDHETFDASVALERMTPVTPQVLD
                     ILAGYDYGMQRTMVNEVEPAHMARRRALIEPFTPAALKALEPMVRRVARQYVDRFIDD
                     GRADLVEKMFWEAPLTVGLHFLGVEEDDMALLRQYSVAHTVNIWGRPTPQEQVEVAHA
                     VGNFWQLSGRIVEKMRATPDGPGWMRYTIRKQKDHPDVVTDSYIHSMMMAIIVAAHET
                     TALGITNAVRLLLENRPIWDELCADPSLIAGAVEECLRHNGSVAAWRRYATREAEIGG
                     VRIPQGARVLVVLASGNHDERHFSDPDAIDVRRDNASEHLTFGYGSHQCMGKNLARLE
                     LQIYLEELTRRLPHLRLLPQRFDYVPNTSFRGPKQLWVEWDPALNPERAQPALLAQAQ
                     PVRLGESSRQTVTRPVVVERISRATDDVLLLRLVAANGQPLPGWSPGAHVDLECGSAD
                     RSRQYSLCSDPADSRAYEIAVLRDPDSRGGSAWIHQHLQVGDRLRLRGPRNHFRFDET
                     GGRAIFVAGGIGITPIAAMARRARQRGIDYALHYSGGSRRRMALLAELQAEHGERLIV
                     HASDEGQRLDLPALLATPRADTRIYACGPQRMLQALQDATAHWPEGALCVEHFQTAPP
                     TLDPAREHAFEVELRDSGITLQVRADQTILSALQGANIDVQCDCTEGLCGSCETRVLG
                     GDIDHRDLVLTRTERERQDRMMVCCSRARGERLVLEL"
     gene            7004..7333
                     /locus_tag="PK242_07405"
     CDS             7004..7333
                     /locus_tag="PK242_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOZ93880.1"
                     /translation="MPYTARFTPETLSRAQVDAQTGPLVLEFGTDWCGHCQGAQPLIE
                     AAFARAGQDTPHLKVEDGRGRPLGRSFGVKLWPTLVFLKDGQEQARLVRPHDADAIAQ
                     ALGQLSA"
     gene            7671..8900
                     /locus_tag="PK242_07410"
     CDS             7671..8900
                     /locus_tag="PK242_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ93881.1"
                     /translation="MLKPLRHPGALAVTLAAAGVLMVTMGTRQSLGLFLSPINSSTGL
                     GMASISLAMAVAQFTWGAIQPLAGAAADRWGPRPVLIAGIVLLALGNALTPLMGSSWG
                     LVFSLGLLAAIGSGAGSFSVLIGAAAQRLPMAARGTASGVINAGGSLGQFVFAPLAQL
                     LIQVQGWMGAMYSLAVITLLALPLVGQLTRGQPARHDAVEGADAGLRATLRGALGDRS
                     YLLLHAGFFTCGFHIAFLVTHLPTEVDLCGLPASVASWSLALIGLSNIVGSLWVGHLV
                     GHHRSKMILALMYGSRALLVLVYLLLPREPWVFYLFAIGLGLTWLATVPPTAALVGKL
                     FGVRYLGTLFGMTLLSHQIGGFLGAWLGGLAITQLGDYTWMWWADIALAALAALLNLP
                     IREARVVPLAASAAEGR"
     gene            complement(8955..10385)
                     /locus_tag="PK242_07415"
     CDS             complement(8955..10385)
                     /locus_tag="PK242_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ93882.1"
                     /translation="MTAADAVFDPVALQAATLDVVAATHGTPRLIAQRQQQRVLRLLE
                     AARASPLYRERLGTERVRGSDLSRLPPVTRRELMARFDDWVTNPALRLDELRDFVSDP
                     SRAGQPWLGRYVVWESSGTSGKPGIFVQDAQAMAVYDALEAVRHRPPCSRGRGMFGAF
                     AAFDVLGVSDRHAVVTATGGHFASIVSFERLRRINPWLTSSGRSFSLLQPVDELVRAL
                     NEFQPTVLGTYPTAAAMLAGEAEAGRLRIAPRCIMTGGETLGKAVRDRLVQVFGAQVR
                     NSYGASEFLPIAWECPHGQLHVNEDWVLLEPVDEQMRPMPPGQRSHSVLLTNLANLVQ
                     PLIRYDMGDQVTWSGQRCSCGSALPVIEVRGRSDDVMQVPSQRRGETVSLLPLALCTV
                     IEEECGVFDFQLRQHGPSTLVLRLPLRGEAAVAAITRCADALQRFAAMQGARPIRVLG
                     EPGCDVPRGRSGKACRVAMEGSNPVQ"
     gene            10674..>11110
                     /locus_tag="PK242_07420"
     CDS             10674..>11110
                     /locus_tag="PK242_07420"
                     /inference="COORDINATES: protein motif:HMM:NF023965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-hydroxybutyrate polymerase N-terminal
                     domain-containing protein"
                     /protein_id="HOZ93883.1"
                     /translation="MARQSAPAIRPARPRIRPAEPAPAHPGYVSDPVNPGDPAETPTQ
                     KLDRLAQSAAASLYNNLSPVTLSLALADWAWHLAASPGRQLELATLAAQLAIDTARLE
                     DGSTNGVGLRDDDPRFRADEWAQWPFNRWRTAFRNAEVFWREAS"
BASE COUNT         1529 a         4124 c         3894 g         1563 t
ORIGIN      
        1 gtcaccgacc gctgcaactt ccgctgcacc tactgcatgc ccaaggaggt gttcggcgcc
       61 gactacccct acctgccgca cgcgtcgctg ctgacgttcg aggaaatcac gcgcctggcg
      121 cgcctgttca tcgcccacgg cgtgcgcaag atccgcctga ccggcggtga gccgctgctg
      181 cgcaagaacg tcgagggtct gatcgagcag ctggcggcgc tgcgcacgcc cgacggcgag
      241 ccgctggaca tcaccctcac caccaacggc tcgctgctgg cgcgcaaggc ccgcgcgctg
      301 gcggcggcgg gcctcaggcg cgtcacggtc agcctggaca gcctggacga tgcggtgttc
      361 cagcgcatga acgacgtcgg ctttccggtg cgcgaggtgc tggccggcat cgaggcggcg
      421 caggccgccg ggctggcgcc gatcaaggtc aacatggtgg tcaagcgcgg caccaacgcg
      481 cacgagatcg tgcccatggc gcggcacttt cgcggcagcg gcatggcgct gcgcttcatc
      541 gaatacatgg acgtgggcgc caccaacggc tggcgcatgg acgaggtgct gccctcggcc
      601 gaggtgctgc ggcgcctgca ggccgaattc gccctggtgc cgctggcgca cagcgcgccg
      661 ggcgaaaccg ccgagcgctg gggccatgcc ggcgccgatg gccgccacga cccggcgctg
      721 ggcgagatcg gcctcatcag cagcgtcacc cgcccgttct gcggcgactg caaccgcgcc
      781 cgcctgtcca ccgaagggcg gctgtacctg tgcctgtttg ccagccaggg ctacgacctg
      841 cgcgccctgc tgcgcggcgg cgccagcgac gaggccatcg gccacgccat cggcgccatc
      901 tggggccatc gcgccgaccg ctacagcgag ctgcgcgcca gcggccggct cgacccgcac
      961 agcggcgagc ggcgcatcga gatgagctac atcggtggct gaccccgcgc cccgggcctg
     1021 gccgagactc gcgtcatgac cgccatccct ggcgcccctg ccgatccgga ccggatcccc
     1081 atcaccgccc tgctgctggc cggcggccgc ggcagccgca tgggtgggct ggacaagggc
     1141 ctgcagagct tcggcggcct gccgctggcc cagcatgcgc tggagcgttt gcgccggcag
     1201 acgttgccgc ccgccgccat gctgatcaac gccaaccgcc acctgcccga ctaccaggcc
     1261 ttcggcgtgc cggtgtggcc cgacgtgctg cccgattacg ccggcccgct ggccgggttc
     1321 ctgaccgggc tggcgcatgc ccagacgccc tggatgctca ccctgccgtg cgacgtgccg
     1381 cgttttccgc tatcgctttg cgagcgcctg gcgcaagccc tgcttgccca aaaggccgat
     1441 atcgccatgg tcgcggcgcc cgaggaggac ggccggctgc gcccccagcc ggtgttctgc
     1501 ctgctgcggc gcgagttgca cgacagcctg gcgcgcttca tggcggcggg cgggcgcaag
     1561 atcgacgcct ggaccgcgca gcaccggcag gtgctggtgc cgttcgacca cccgggcgac
     1621 gacccgcgcg cctttgccaa cgccaacacg ctggccgagc tgcgccagct ggagcgcgaa
     1681 ccccgacaag acgcatgcca gccccccatg cccagcttct gaccccggag acaccaccat
     1741 gccgctgccg ccgctgacca tccgcctgct cggcccggcc gacctgcccg cctacaaggc
     1801 gctgcgcgac atcggcctgc gccacgaccc ggaggccttc accaccgact tcaacgccgg
     1861 ctcggccctg ccggccgccg cctacgccac gcgcctgggg caaccgccgg aagaccactt
     1921 catcctgggc gccttcgacc ccgccggcgc catgctgggc gccgtggtgt gcgagcgcga
     1981 gtcgcgcctg aaaaaacgcc acgaagccgc cctggtgggc atgatcatcg cccccgaggt
     2041 gcgcagccac ggcatcggca aggcgctgct ggccgcgttc gaccagctgg tgcgccagct
     2101 gccggggctg gagcaggtgg tgctgtcggt caccgccagc aacgcgcgcg ccgtggccct
     2161 gtacgagcac gccggcttcg tgcgctatgg cctgctgccg cgcgccatca agatcgatgg
     2221 ccgctaccac gacaaggcgc tgatggtcaa agtgctatga ccccgaccac ggccgccacc
     2281 accccgcgca ccgccgacat cgccgccgcg ctgcagggct acgacccgca ggcgctgcac
     2341 gccgacgccg tgaccgcctt tctgcagcgc ctggtcgagc ccgtcaccga agtgcagcgc
     2401 gtgccgctgc ccgaggcgct gggccgggtg ctggccgaag acgtgatctc gcccatcagc
     2461 gtgccgccgc acgacaactc ggccatggac ggctttgcct tcgacggcgc gcaactgcaa
     2521 cccggccagc cgctgcgcct gcgctgcgtc ggcacggcgc tggccggcac cgcctggcgc
     2581 gctgcggtgg cggccggcga atgcgtgaag atcatgaccg gcgccgtcat gccggccggg
     2641 ctggacaccg tggtgccgca ggaattcacc cgcgtcgagg gcgcgcagat cgagctgccg
     2701 gccggcgtgg tgcagcgcgg cgacaaccgc cgccaggccg gcgaagacct gatggccggc
     2761 cgcccggcgc tgcgccaggg cgagcgcctg accccggccg cgctcggcct gctggccagc
     2821 ctcgggctgg gccaggtgac gctgcggcgg cgcctgcgcg tggcctactt ctcgaccggc
     2881 gacgaaatcc tgtcgctcgg ccagcccccc cgcgaaggcg cggtgtacga cagcaaccgc
     2941 tacacggtcg gcggcctgct ggcacgcctg ggggtcgaag tgatcgacct cggcgtggtg
     3001 cccgaccagc cggccgcgct gcgggcggcg ttcggccagg ccgccgcaca ggccgatgcc
     3061 gtcatcacca gcggcggcgt cagcgtgggc gaagccgacc acaccaaggc catgatgcgc
     3121 gagctgggcg acgtggtgtt ctggcgcatc gccatgcggc caggccggcc gatggcggtc
     3181 gggcggatcg tcaccccggc cccggagtta caagtcaaat cggcctccag cgcaaacaca
     3241 gcaaacgcca acagctatca aaacaatagt aattcagcga tcctgttcgg cctgccgggc
     3301 aacccggtgg cggtgatggt gacctttctg gccttcgtgc ggccggcgct gctgcgcatg
     3361 atgggcgcgc ggcccgagcc gccggtgtgg ctgcgcgcgc gcagcagcca ggcgatcgcc
     3421 aagaagcccg gccgcaccga ataccagcgc ggcgtcgtca gcacccagcc cgatggccag
     3481 ctgacggtgc gcaccaccgg caaccagggc tcgggcgtgc tgtcgtccat ggtgcaggcc
     3541 aacggactga tcgtgctcgg ccacgcccag ggcgacgtgg cggtgggcga cgaagtgcgg
     3601 gtgatgatgt tcgagggcgc gctgtagccc cgcccccgcg gcgactcagg gcgcgcgcat
     3661 cagcaccggc acgccggcct ggcgcaggca gccggcggtc acgctgccca gcagggcggt
     3721 ttcgaccgcg ccctggcggc gcgtgaccat cgccagcacg gtgtcgctgc gcacggccac
     3781 gtggctggca atggcctgca ccgcgtcgcc atgcagcacg tcccagttga ccggcacgcc
     3841 gtggctgcga cccagctcgg tggccttgct gcgcacgtag cccgattcca gcgccgccaa
     3901 ctcgccgccc gccggcagtc cggccgcgcc ggtggcgtcg accacccgca ccacctccag
     3961 ctcggcgccg gcccagcggg cgaattcggc cgcctgcagg cccatgacct cggacagcgg
     4021 gctgccgtcg agcggcagca cgatgcgccg caccgccacc gcgccgccgc cggcggccga
     4081 gcgaccagtc ggccggtaca ccagcaccgg cgcgtcggca ccgcgcacca cgcgctgcgc
     4141 cacgctgccc agcaccgcct ccatcaggcc cgagcgcccg cgcgaggaca tcgccaccag
     4201 cgtgtgcggc acacccgcag cctcgtccag caccgtgtcg gccacgtcgc catgcgccac
     4261 cacgcagatg ccgcgtgccc catggcgggc cgccagagcc tcgacgtagg cgcgcgcctc
     4321 ggcctcgtcg gcgcccttgt ccaccacccg catcagcgtc agcggcgtgc cgcgcaccgc
     4381 cgccaggccg gtggcgtggg ccagcatctc ttcggaaaac accgagccat ccaggggcac
     4441 cagaatccgt tcgaacatgc gctctcctgc gtccttgaca ctgcaccggc cccgagtgta
     4501 ggccaggccg cccccgggct gggtcaccct agatccaggg tcacagctcc agcaccaggc
     4561 gctcgccgcg ggcgcgcgag cagcacacca tcatgcggtc ctggcgctcg cgctcggtgc
     4621 gcgtcagcac caggtcgcgg tggtcgatgt cgccacccag cacgcgcgtc tcgcaggagc
     4681 cgcacagacc ttcggtgcaa tcgcactgca cgtcgatgtt ggcgccctgc agcgccgaca
     4741 ggatggtctg gtcggcacgc acctgcaggg tgatgccgga gtccctgagc tcgacctcga
     4801 aggcgtgctc ccgggccgga tcgagggtgg gcggcgcggt ctggaaatgc tcgacgcaca
     4861 gcgcaccttc gggccagtgg gcggtggcgt cctgcagcgc ctgcagcatg cgctgcgggc
     4921 cgcaggcata gatgcgggtg tcggcgcgcg gcgtggccag cagcgccggc aggtcgaggc
     4981 gctggccctc gtcgctggca tgcacgatca ggcgctcgcc gtgttcggcc tgcagttcgg
     5041 ccagcagggc catgcggcgg cggctgccgc cgctgtagtg cagtgcgtag tcgatgccgc
     5101 gctgccgggc gcgccgcgcc atggcggcga tcggcgtgat gccgatgccg ccggccacga
     5161 agatggcgcg gccgcccgtt tcgtcgaaac ggaagtggtt gcgcgggccg cgcaggcgca
     5221 gccggtcgcc gacctgcaga tgctggtgga tccaggccga cccgccacgg ctgtcggggt
     5281 cgcgcagcac ggcgatctcg taggcgcggc tgtcggccgg gtcgctgcac agcgagtact
     5341 ggcgcgaacg gtcggcgctg ccgcattcga ggtcgacatg ggcgcccggc gaccagccgg
     5401 gcagcggctg gccgttggcg gccaccaggc gcagcagcag cacgtcgtcg gtggcacggc
     5461 tgatgcgctc gacgaccacc gggcgggtga cggtctggcg gctggattcg cccagccgca
     5521 ccggctgcgc ctgcgccagc agcgcgggct gggcacgctc ggggttgagc gccgggtccc
     5581 actcgaccca cagctgcttg ggcccgcgaa acgaggtgtt gggcacgtag tcgaagcgct
     5641 gcggcagcag gcgcaggtgc ggcaggcggc gcgtcagctc ctccaggtag atctgcaact
     5701 ccagccgcgc caggttcttg cccatgcact ggtgcgagcc gtagccgaag gtcaggtgct
     5761 cgctggcgtt gtcgcgccgc acgtcgatgg cgtcggggtc gctgaagtgg cgctcgtcat
     5821 ggttgcccga ggccagcacc accagcacgc gcgcgccctg gggaatgcgc acgccgccga
     5881 tctcggcctc gcgcgtggcg tagcgccgcc aggccgccac cgagccgttg tggcgcaggc
     5941 actcctcgac cgcgccggcg atcaggctgg ggtcggcgca cagttcgtcc cagatcgggc
     6001 ggttttccag cagcaggcgg accgcgttgg tgatgcccag ggccgtggtt tcgtgcgccg
     6061 ccacgatgat ggccatcatc atcgagtgga tgtaggagtc ggtgacgacg tcggggtggt
     6121 ccttctgctt gcggatggtg tagcgcatcc agcccggccc gtcgggcgtg gcgcgcatct
     6181 tctcgacgat gcgccccgac agctgccaga agttgcccac cgcgtgcgcc acctcgacct
     6241 gctcctgcgg cgtcggccga ccccagatgt tgacggtatg cgccaccgaa tactggcgca
     6301 gcagcgccat gtcgtcttcc tccaccccca ggaagtgcag gccgacggtg agcggcgcct
     6361 cccagaacat cttctcgacc aggtcggcgc ggccgtcgtc gatgaagcgg tccacgtact
     6421 ggcgcgccac gcgccgcacc atgggttcga gcgccttgag cgccgccggc gtgaagggct
     6481 cgatcagcgc ccgccggcgc gccatgtggg ccggctccac ttcgttgacc atggtgcgct
     6541 gcatgccgta gtcgtagccg gccaggatgt ccagcacctg cggcgtcacc ggggtcatgc
     6601 gctccagcgc caccgaggcg tcgaaggttt cgtggtcgcg gaacaccgcc ttgatgtcgt
     6661 cgtagcgcgt gaccacccag tagccgatct gcggcgcaaa gaacaccggc tcctgggcgc
     6721 gcgcccagcg caggtactcg ggcgggttct gctggtagcc gtcgccgaac gggtcgaagg
     6781 cggcggcgcc ggcgctgacc gggcggccgt tggggtcgcg cagggcggca tggtccaccg
     6841 ggcaggcgcc ggcggcaggg tgggtctcgc tcatggtggt gctcttgctc gaagccaggg
     6901 aacgcaaggc tactacgttg cacggccatc ggccaggcgc cgctgcagcg cctcggcgcg
     6961 ctggctatga tgcccgggca ccgatcaccg cagcgcagcc gccatgccct acaccgcccg
     7021 cttcaccccc gagaccctga gccgcgccca ggtcgacgcc cagaccggcc cgctggtgct
     7081 ggaattcggc accgactggt gcggccattg ccagggcgcg caaccgctga tcgaggcggc
     7141 cttcgcgcgc gccggccagg acacgccgca cctcaaggtg gaggacgggc gcgggcggcc
     7201 gctgggccgc agcttcggcg tcaagctgtg gccgaccctg gtgttcctga aggacggcca
     7261 ggagcaggcg cgcctggtgc gcccgcacga cgccgacgcc atcgcccagg cgctgggcca
     7321 gctcagcgcc tgatgcgaac cgcccatgag ggagagcctc gctgcgcttc gatggcccat
     7381 gggtcatgtt cctgaacgcc atgccgcatg cacaccgcgc caccctggcc actgcccccg
     7441 atcgccccga ccgcacgccc ggcgcggcct gggtgggtgg acggcacctg cggcggggcc
     7501 gacacccgcc gggccgggcc cctcgagcgg cacggccgcc cagcccgtca cggcgccctg
     7561 gaacatgaac aaaatcatgt tccagcgcat gactggcaag cgccagcagc tattgttttg
     7621 atagtatccg ggcaccccgc ccggccccgg ccagaggcca gaaggacccc atgctgaaac
     7681 cactccgaca tcccggcgcg ctggcggtga cgctggccgc cgccggcgtg ctgatggtga
     7741 ccatgggcac gcgccagtcg ctgggcctgt ttctgagccc gatcaacagc agcaccgggc
     7801 tgggcatggc cagcatcagc ctggccatgg cggtggcgca gttcacctgg ggcgccatcc
     7861 agccgctggc cggtgcggcg gccgaccgct gggggccgcg accggtgctg atcgccggca
     7921 tcgtgctgct ggcgctgggc aacgcgctga cgccgctgat gggttcgtcc tggggtctgg
     7981 tgttcagcct gggcctgctg gcggccatcg gttcgggcgc gggcagcttt tcggtgctga
     8041 tcggcgccgc cgcccagcgc ctgccgatgg cggcgcgcgg cacggcctcg ggcgtcatca
     8101 acgccggtgg ttcgctgggg cagttcgtgt tcgcgccgct ggcgcagctg ctgatccagg
     8161 tgcagggctg gatgggggcc atgtattcgc tggccgtcat caccctgctg gcgctgccgc
     8221 tggtcggtca gctcacgcgc ggccagccgg cgcggcacga cgccgtcgaa ggcgccgacg
     8281 ccggcctgcg cgccacgctg cgcggcgcgc tgggcgaccg cagctacctg ctgctgcacg
     8341 cgggcttttt cacctgcggc tttcacatcg ccttcctggt cacgcacctg ccgaccgagg
     8401 tggacctgtg cggcctgccg gcctcggtgg ccagctggtc gctggcgctg atcggcctgt
     8461 ccaacatcgt cggcagcctg tgggtcggcc acctggtggg ccaccaccgc agcaagatga
     8521 tcctggccct catgtacggc agccgggcct tgctggtgct ggtctatctg ctgctgccgc
     8581 gcgagccctg ggtgttctac ctgttcgcca tcggcctggg cctgacctgg ctggccacgg
     8641 tgccacccac cgccgccctg gtgggcaagc tgttcggcgt gcgctacctg ggcacgctgt
     8701 tcggcatgac gctgctgagc caccagatcg gcggttttct gggcgcctgg ctgggcgggc
     8761 tggccatcac ccagctgggc gactacacat ggatgtggtg ggccgacatc gcgctggcgg
     8821 cgctggccgc gctgctgaac ctgccgatcc gcgaggcgcg cgtggtgccg ctggcagcgt
     8881 cggccgccga aggcaggtga tcttgaggtg atttcgtgcc tcaggccttg ccagtcaagc
     8941 gccggaagct atcattattg aaccgggttt gacccctcca tcgccacccg gcaggccttg
     9001 ccgctgcgcc cgcgcggcac gtcgcagccg ggttcaccca gcacgcggat cggccgcgca
     9061 ccctgcatgg cggcaaagcg ctgcagggca tcggcacagc gggtgatggc ggcgacggcc
     9121 gcttcgccgc gcaggggcag gcgcagcacc agggtgctgg ggccgtgctg gcgcagctga
     9181 aagtcgaaca ccccgcactc ctcctcgatc acggtgcaca gcgccagcgg cagcaggctg
     9241 acggtctcgc cacggcgctg gctgggcacc tgcatgacat cgtcgctgcg cccacgcacc
     9301 tcgatcaccg gcagcgccga gccgcagctg cagcgctggc cgctccaggt gacctggtcg
     9361 cccatgtcgt agcggatcag cggctgcacc aggttggcca ggttggtcag cagcacgctg
     9421 tgcgaacgct ggcccggcgg catcgggcgc atctgctcgt ccaccggctc cagcagcacc
     9481 cagtcctcgt tcacgtgcag ctggccgtgc gggcattccc aggcgatcgg caggaactcc
     9541 gacgcaccgt agctgttgcg cacctgcgcg ccgaacacct gcaccagccg gtcgcgcacc
     9601 gccttgccca gggtttcgcc gccggtcatg atgcagcgtg gcgcaatgcg cagccggccg
     9661 gcttcggcct cgcccgccag catggccgcc gccgtcgggt aggtgcccag caccgtgggc
     9721 tgaaattcat tgagcgcccg caccagctcg tcgaccggct gcagcagact gaagctgcgc
     9781 cccgacgagg tcagccaggg gttgatgcgg cgcagccgct cgaagctgac gatgctggcg
     9841 aaatgcccgc cggtggccgt caccaccgcg tgccggtcgc tcacgcccag cacgtcgaag
     9901 gcggcgaagg cgccgaacat cccgcgcccg cggctgcacg gcggccggtg gcgcaccgcc
     9961 tccagcgcgt cgtacaccgc catggcctgc gcatcctgca cgaagatgcc gggcttgccg
    10021 ctggtgcccg agctttccca caccacgtag cgccccagcc agggttggcc ggcgcgcgag
    10081 gggtcgctga cgaagtcgcg cagttcgtcc agccgcagcg ccgggttggt cacccagtcg
    10141 tcaaagcgcg ccatcagctc gcgccgcgtc accggcggca ggcgcgacag atcggagccg
    10201 cgcacccgtt cggtgcccag gcgctcgcgg tacagcggcg aggcgcgcgc cgcctccagc
    10261 aggcgcagca cgcgctgctg ctggcgctgg gcgatcaggc gcggcgtgcc gtgggtggcg
    10321 gccaccacgt ccagcgtggc ggcctgcagg gccacggggt cgaacacggc gtcggccgcc
    10381 gtcatcggcg gtcccgggtc aggccgccgc acaggtgccc cgtgcggccc gcgttgcagt
    10441 gaaccatggt ggtcggatcc tcttgatgcg gggcgcaggt cggacgaccg gccgtcgccc
    10501 caggggtggg ctcccatgat gcaacatttc tcacgctctg cggcggttcg tgcgaccatc
    10561 gtgccgcagg tggccggtgg catcttgacc gaagtcaatg gtccagcggg ccgctccggt
    10621 attcttgtct ttggtcccct gcgtccgatt ccgcaccttg cacgagaacc gccatggccc
    10681 gtcagagcgc tcccgccatc cgtccggccc gcccgcgcat ccgccctgcc gagcccgcgc
    10741 ccgcgcaccc cggctacgtc agcgatccgg tcaaccccgg cgatccggcg gaaaccccga
    10801 cccagaagct cgatcgcctg gcgcaaagcg cggcggcctc gctctacaac aacctgtcgc
    10861 cggtcacgct gagcctggcg ctggccgact gggcctggca cctggcggcc tcgcccggcc
    10921 ggcagctgga gctggccacg ctggcggcgc aactggcgat cgacaccgcg cgtctggaag
    10981 acggcagcac caacggcgtc ggcctgcgcg acgacgaccc gcgctttcgc gccgacgaat
    11041 gggcccagtg gcccttcaac cgctggcgca ccgccttccg caacgccgag gtgttctggc
    11101 gcgaagcctc
//
LOCUS       DAOOOG010000080        10974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6733_length_10974_cov_23.149647, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000080 DAOOOG010000000
VERSION     DAOOOG010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10974
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6733_length_10974_cov_23.149647"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PK242_07425"
     CDS             <1..272
                     /locus_tag="PK242_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOZ93884.1"
                     /translation="ISVTANVAPRLMHELCVAAIAGDRRRAMEIQFKLMPLHKHLFVE
                     ANPIPVKWAMARLGLCGGALRLPMTRLSKACEPAVEQALRDSGLL"
     gene            340..1458
                     /gene="bamC"
                     /locus_tag="PK242_07430"
     CDS             340..1458
                     /gene="bamC"
                     /locus_tag="PK242_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HOZ93885.1"
                     /translation="MLNVTFIPRSCLPLLGVAVLAASGCSVLEPDRIDYKSAGRGVAL
                     EVPPDLSQLPGQSRYAAPGGSVTASAYQAGQAATVAAGTPTAANRIADVHFMRQDGER
                     WLRVDRPADKLWGDVRDFWLESGFLLAVDQPSMGLMETDWAENRAKIPQDIIRQTIGK
                     VFDSLYSTGERDKFRIRLERNPAGGTDIFVTHRGMEEVYSSARRDSTIWQPRARDPEL
                     EAEFLRRLMVRLGVSQEQSKAALAAAAAPTATPQARLVTAANGTPQVQLAEDFDRAWR
                     RVGLALDRTGFTVEDRDRSKGIYFVRYVDPSLEKQEPGFFGKLFGRGSAPLPTQQYQV
                     VVRSEGQSSQVSVLDRAGGAAPGADAQRIVKVLADELK"
     gene            1483..2259
                     /locus_tag="PK242_07435"
     CDS             1483..2259
                     /locus_tag="PK242_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ93886.1"
                     /translation="MLRFRSLASGSAGNATLLEADDGLRRTRVLIDCGLGLRPLTARL
                     AAEGLALTDLDGVFITHEHGDHLGCARSLMRRHGVPLWTSAGTARSLDTPDAGAARPR
                     LVRAGETFAIGGIELHPFAVPHDAREPLQLRCTDGARSLGVLTDLGHVTPQVLDALAG
                     VHALLLESNHDSELLARGDYPDFLKRRVGGTHGHLSNAQAAAALTALGHAQLTTVVAA
                     HLSERNNRPELVRQRFAAVLGCAAGEVLLAERHGTCWLGV"
     gene            complement(2374..2700)
                     /locus_tag="PK242_07440"
     CDS             complement(2374..2700)
                     /locus_tag="PK242_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93887.1"
                     /translation="MNKSLLLAALIAAASLAACGKKEEPAPAPAPAAEAPAAPAAAAA
                     ASAVDAAASAVQGAASDAAAAVTNAASTAATAAGDAASAAAAAATDAAAAAANAAKDA
                     ASAAAK"
     gene            complement(2886..3389)
                     /locus_tag="PK242_07445"
     CDS             complement(2886..3389)
                     /locus_tag="PK242_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93888.1"
                     /translation="METEAPPSPLPSGSFIGHESFRNHLRTGLLAAAQQGWRELLLSD
                     ADFRHWPLGEREVVEALTAWVRGARRFTMLAKSYDEVPRLHARFVEWRRLWSHRIDCR
                     VCREADATELPSALWTPGWTVQRLDLLHSSGLCGDDPARRLLLREQIDGWLVRSTPGF
                     PAYTLGL"
     gene            complement(3379..4500)
                     /locus_tag="PK242_07450"
     CDS             complement(3379..4500)
                     /locus_tag="PK242_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOZ93889.1"
                     /translation="MQIDQPLTLLGGLSPARFMHRHWQKKPLLVRQAMPGFKPLLSRA
                     ELFALAGDDAVESRLVRRSSSGWQMRRGPLARRALPPLQQADWTLLVQGVDLHHQPAH
                     ELLQAFRFVPDARLDDLMISYASDGGGVGPHFDSYDVFLLQAHGRRRWRIGRQKDLTL
                     VEGLPVKILADFRPQQEFLLEPGDMLYLPPGWAHDGVAVGECMTYSIGFRQPARDEMV
                     RELLQRVADDATDLVGEAVYRDPAQPASAQPAQLPEAMLAFARDALQCALEQPDHLAL
                     LLGEWLTEPKPQVWFEAGDGAGQVQAGVRLDRRTRMLYDARHVFINGEGFRASGTDAR
                     LMRALADARQLTAAQVGRLGADARALLQQWHRAGWLHGN"
     gene            4556..5080
                     /locus_tag="PK242_07455"
     CDS             4556..5080
                     /locus_tag="PK242_07455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOZ93890.1"
                     /translation="MEITPQCVVALTWVLKDSLGDTLDELHEPVEFLVGSEDLLPVLS
                     AALQGHESGATLNLNLEPEDAFGDFDEQLVFLARRDQLPPGIEEGMLIEAASLPAGSV
                     AQAPADTLFTITEIYPEHVVLDANHPLAGMALRLQLKVVAVREALDDELERGSAGAGF
                     FRLQPMAPGSPHLH"
     gene            complement(5135..5611)
                     /locus_tag="PK242_07460"
     CDS             complement(5135..5611)
                     /locus_tag="PK242_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HOZ93891.1"
                     /translation="MHLSQRFVRSLATAAAALTLAACAQHGGYPQGNYPGSYPGSYPG
                     NQAYSQFGRVTNVEYVRGGQSQGVAGAVIGGAVGGLAGSQVGGGSGRTAATVAGVIGG
                     ALIGNMLERNMDRNRVEHYRVTVQFDNGGVRQFEYAQHPNVQIGDRVRAEGDQLYR"
     gene            complement(5717..6163)
                     /gene="dut"
                     /locus_tag="PK242_07465"
     CDS             complement(5717..6163)
                     /gene="dut"
                     /locus_tag="PK242_07465"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOZ93892.1"
                     /translation="MRLDVRILDQRLRTQMPAYATPGSAGLDLRACLDAPLTLAAGAW
                     QLVPTGMAIHLADPGYAALILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSAWNRSA
                     SAFTIEPMERIAQLVIVPVVQASFQIVDAFEASQRGAGGYGSTGRG"
     gene            complement(6160..7392)
                     /gene="coaBC"
                     /locus_tag="PK242_07470"
     CDS             complement(6160..7392)
                     /gene="coaBC"
                     /locus_tag="PK242_07470"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOZ93893.1"
                     /translation="MDFAGELQGRHIVLGLSGGAACFKAAEYCRLLTKAGATVQVVMT
                     EAACQFITPVAMQALSGRPVYVSQWDARPDNAMAHINLSREADAIVVAPASADFIAQL
                     AQGRAGELLALLCLARPIEQVPLLLAPAMNREMWAHPATQRNLRQVAQDGAQVLGVAH
                     GAQACGEFGDGRMLEAAELLEELIAFLAPKSLLGQRVLITAGPTFEAIDPVRGITNLS
                     SGKMGFAIARAAREAGAEVTLVAGPVHLPTPRGVHRIDVGSARQMHAAATGHAQHAHI
                     FIATAAVADWRPASAAEHKIKKDGSGQVPQLGFVENPDILADIAHSARAQAGELYCVG
                     FAAESQDLQANAQAKRVRKGVPLLVANLGPATFGKDDNALLLVDDEGVTELPRASKRH
                     LAQQLIHEIARRVSGLDA"
     gene            7481..9154
                     /locus_tag="PK242_07475"
     CDS             7481..9154
                     /locus_tag="PK242_07475"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOZ93894.1"
                     /translation="MTKFVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYI
                     NVDPGTMSPIQHGEVFVTDDGAETDLDLGHYERFITTRMKKANNFTTGQIYQSVLEKE
                     RRGDYLGKTVQVIPHITGEIQDFIRRGAGVGTPDAVDVAIVEIGGTVGDIESLPFLEA
                     VRQLSLKLGPNHSAFVHLTYLPWIAAAGELKTKPTQHTVQKLREIGIQPDALLCRADR
                     TIPQDEREKISLFTNVPAWGVISMPDVDTIYKVPRLLHQQGLDGLICDKLRLNTPPAN
                     LKRWDALVHEVESPRHEVSIAMVGKYVDLSDSYKSVNEALRHAGMHNHARVHIEHIDS
                     ETITPATAAQLARFDAILVPGGFGERGVEGKIATVQYAREHKLPYLGICLGMQVATIE
                     YARHVAGLEGANSTEFNPATRHPVIALVTEWKDKDGRIQRRDAGSDLGGTMRLGAQSA
                     DVVPGTLAHRIYGDVVTERHRHRYEANVDYLERLRAAGLVISALTQREQLTEIVELPQ
                     GVHPWFVGVQFHPEFKSTPWDGHPLFNAFIEAALRHQASRRAAAPKETA"
     gene            9151..10008
                     /gene="kdsA"
                     /locus_tag="PK242_07480"
     CDS             9151..10008
                     /gene="kdsA"
                     /locus_tag="PK242_07480"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOZ93895.1"
                     /translation="MKLCGFDVGLRRPFFLIAGPCVVESEQLQMDVAGQLKEITASLG
                     IPFIFKSSYDKANRSSGASFRGPGMDAGLQILARVRREIGVPVLTDVHTEAEVPTVAA
                     VVDVLQTPAFLCRQTDFIRAVAQSGKPVNIKKGQFLAPHDMVNVIDKARAAAAEKGLD
                     TDRFMACERGASFGYNNLVSDMRSLAIMRETGAPVVFDATHSVQLPGGLGQASGGQRE
                     MVPVLARAAVAVGVAGLFMETHPDPARALSDGPNAVPLRHMKALLETLVALDQVTKRQ
                     GFLEDDFGA"
     gene            10012..10302
                     /locus_tag="PK242_07485"
     CDS             10012..10302
                     /locus_tag="PK242_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HOZ93896.1"
                     /translation="MPAGYIIALVTITNPQQYEEYKRWSSEAMRVHGAEICVRGDRVE
                     VLEGDWHPGRTVILKFPSFEAAKAFHDSPEYSRARAARQGAAVMRLLCVEGV"
     gene            10386..>10974
                     /gene="eno"
                     /locus_tag="PK242_07490"
     CDS             10386..>10974
                     /gene="eno"
                     /locus_tag="PK242_07490"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559578.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOZ93897.1"
                     /translation="MSAIVDIVGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     SREAIELRDGDKKRYLGKGVLKAVEHINTEISEAVLGLDAAEQAFLDKTLIDLDGTDN
                     KSRLGANALLAVSMAVARAAAEESGLPLYRYFGGMNGCQLPVPMMNVINGGAHANNNL
                     DLQEFMIIPVGAPSFREALRWGAEVFHALKKIIHDK"
BASE COUNT         1803 a         3883 c         3697 g         1591 t
ORIGIN      
        1 acatcagcgt caccgccaac gtggcgccgc gcctgatgca cgagctgtgc gtggccgcca
       61 tcgcgggcga tcgtcgccgg gcaatggaaa tccagttcaa gctgatgccg ctgcacaagc
      121 acctgttcgt cgaagccaac ccgatccccg tcaaatgggc catggcccgc ctgggcctgt
      181 gcggcggcgc gctgaggctg cccatgacgc ggctgtccaa ggcctgtgaa cccgcggtgg
      241 agcaggccct gcgcgacagt ggcctgcttt gattactaca atatccatag ctgcttgcgc
      301 ttgcccggcg ggcgccggaa cccgatttct ttcaaaggaa tgctgaacgt gacgttcatc
      361 ccccgctcct gcctgcccct gctgggcgtg gccgtgctgg ccgccagcgg ctgctccgtg
      421 ctggagcccg accggatcga ctacaagagc gccggccgcg gcgtggcgct ggaagtgccg
      481 cccgacctgt cgcaactgcc cggccagtcg cgctacgccg ccccgggcgg cagcgtcacc
      541 gccagcgcct accaggccgg gcaggcggcc accgtggcgg cgggcacgcc cacggcggcc
      601 aaccgcattg ccgacgtgca cttcatgcgc caggacggcg agcgctggct gcgcgtggac
      661 cggccggccg acaaattgtg gggcgacgtg cgcgacttct ggctggaaag cggtttcctg
      721 ctggccgtcg atcagcccag catgggcctg atggaaaccg actgggccga aaaccgcgcc
      781 aagatcccgc aggacatcat ccggcagacc atcggcaagg tcttcgattc gctctactcc
      841 accggcgagc gcgacaagtt ccgcatccgg ctggagcgca accccgccgg cggcaccgac
      901 atctttgtca cccaccgcgg catggaagag gtgtattcct cggcccggcg cgacagcacc
      961 atctggcagc cgcgcgcgcg cgaccccgag ctggaagccg aattcctgcg ccgcctgatg
     1021 gtgcgtctgg gcgtctcgca ggagcagtcc aaggccgcgc tggcagcggc cgcagccccg
     1081 acggcgacgc cgcaggcgcg cctggtgacc gccgccaacg gcacgcccca ggtgcaactg
     1141 gccgaagact tcgaccgcgc ctggcgccgc gtcggcctgg cgctggaccg caccggcttc
     1201 accgtcgaag accgcgaccg cagcaaaggc atctacttcg tgcgctacgt cgatccgtcg
     1261 ctcgaaaagc aggaacccgg cttcttcggc aagctgttcg gccgcggcag cgcgccgctg
     1321 ccgacgcagc agtaccaggt ggtggtgcgc agcgagggcc agagcagcca ggtcagcgtg
     1381 ctcgaccgcg ccggtggcgc cgcccccggg gccgacgccc agcgcatcgt caaagtcctg
     1441 gccgacgaac tgaagtagac acggcgcagc ggcggcaccg agatgctgcg ctttcgcagc
     1501 ctggccagcg gcagcgccgg caacgccacc ctgctcgagg ccgacgacgg cctgcgccgc
     1561 acccgggtgc tgatcgactg cgggctgggc ctgcggccgc tgacggcccg cctggccgcc
     1621 gaagggctgg cgctgaccga tctggatggc gtcttcatca cccacgagca cggcgaccac
     1681 ctgggctgcg cgcgcagcct gatgcggcgc catggtgtcc cgctgtggac cagtgccggc
     1741 acggcccgct cgctggacac gcccgacgcg ggcgctgcgc gcccgcggct ggtgcgcgcc
     1801 ggagaaacgt ttgccatcgg gggcatcgag ctccacccct ttgccgtgcc gcacgacgcg
     1861 cgcgaaccgc tgcaactgcg ctgcaccgac ggcgcccgct ccctcggcgt gctcaccgat
     1921 ctgggccatg tcaccccgca ggtgctcgac gccctggccg gcgtgcacgc gctgctgctg
     1981 gaaagcaacc acgacagcga actgctggcg cgcggcgact accccgactt cctcaagcgg
     2041 cgcgtgggcg gcacccacgg ccatctcagc aacgcgcagg ccgcagccgc cctgactgcg
     2101 ctcggccatg cgcagctgac caccgtggtg gcggcccacc tgagcgaacg caacaaccgg
     2161 cctgaactgg tgcgccaacg gttcgccgcc gtgctcggct gcgccgccgg cgaggtgctg
     2221 ctggccgagc ggcacggcac ctgctggctc ggagtctaga tggcctgccc cagacgcaaa
     2281 aaagccaccc gaaggtggct gatttgcaca ggagtgcggc gctggaactg gccgatcagg
     2341 cctgcggcgc aggcaccccg gacccgcaag gggttacttg gcggcggcgc tggccgcgtc
     2401 tttggcggcg ttggcggcag cggcagcggc gtcggtcgca gcggcggcgg cggccgaagc
     2461 agcgtcgccg gcagcggtgg ccgccgtcga agcggcgttg gtcacggcgg cagcggcatc
     2521 gctggcggcg ccctgaaccg cgctggcggc ggcgtcaacc gccgaagcgg cggcggcagc
     2581 ggcaggagca gccggtgctt cggccgcagg agccggcgcc ggagcgggct cttccttctt
     2641 gccgcaggcg gcgagcgaag cggcggcgat cagggcggcc aacaggaggg atttgttcat
     2701 gtcaaggttc ctaggtcaag aaaagaattg ccggatggcg caaagacacc ggccagtgtt
     2761 ccggcgccct gctgcacgga gggagaagca caaaacaccg cttccactca ggccttaatt
     2821 atatggggca tgtcgatcaa tggtgacaaa ccctgatgcg cggacgcgac gccggtgtgc
     2881 aaacgtcaca gaccgagggt gtaggcagga aaccccggcg tgctgcgcac cagccagccg
     2941 tcgatctgct cgcgcagcag caggcgtcgc gccggatcgt cgccgcacag accgctgctg
     3001 tgcagcagat ccagccgctg cacggtccag ccgggtgtcc acagggcgct gggcaactcg
     3061 gtcgcgtcgg cctcgcggca cacccggcaa tcgatcctgt gcgaccacag gcggcgccac
     3121 tcgacgaagc gtgcgtgcag gcgcggcacc tcgtcgtagc tcttggccag catggtgaag
     3181 cggcgtgcgc cacgcaccca cgccgtcagt gcttcgacca cctcgcgttc gcccagcggc
     3241 cagtgccgaa aatcggcgtc cgacagcagc agctcacgcc agccctgttg cgccgccgcc
     3301 agcaggccgg tgcgcagatg gttgcggaaa ctctcgtgtc cgatgaagct gcccgaaggc
     3361 agcgggctgg gcggggcttc agtttccatg caaccacccg gcccgatgcc attgctgcaa
     3421 cagtgcgcgc gcgtcggcgc cgagccggcc cacctgcgcc gcggtcagtt gccgcgcgtc
     3481 ggccagcgcg cgcatcaacc gcgcgtcggt gccgctggcg cgaaagccct cgccattgat
     3541 gaacacatgg cgcgcgtcgt agagcatgcg tgtgcggcga tcgagtcgaa cgccggcctg
     3601 cacctgcccg gcaccgtcgc cggcctcaaa ccagacctgg ggcttgggct cggtcaacca
     3661 ctcgcccagc agcagcgcca ggtggtcggg ctgctcaagc gcacactgga gggcatcgcg
     3721 ggcaaacgcc agcatggcct cgggcagctg tgccggctgc gcgctggccg gttgggccgg
     3781 atcgcggtac accgcctccc cgaccaggtc ggtggcgtcg tcggccacgc gctgcagcag
     3841 ctcgcgcacc atttcgtcgc gcgccggctg gcgaaagccg atcgagtagg tcatgcattc
     3901 gccgaccgcc accccatcgt gcgcccagcc gggcggcagg tagagcatgt cgcccggttc
     3961 gagcaggaac tcctgctggg gccggaaatc ggccaggatt ttgaccggca agccttcgac
     4021 cagcgtcagg tccttctgtc ggccgatgcg ccagcggcgc cgaccatgcg cctgcagcag
     4081 gaacacgtcg tagctgtcga agtgcggacc gacgccgccg ccgtcgctgg cgtagctgat
     4141 catcagatcg tccaggcgcg catcgggcac gaagcggaag gcctgcagca gttcgtgcgc
     4201 cggctggtga tgcaggtcca cgccctgcac cagcagcgtc cagtcggcct gctgcaaggg
     4261 cggcagcgcg cggcgcgcca acggtccacg gcgcatctgc cagccggagg atgaacggcg
     4321 caccaggcgc gactccaccg cgtcgtcgcc cgccagcgcg aacagctcgg cgcgcgacag
     4381 caagggcttg aagccaggca tggcctggcg caccagcaag ggctttttct gccagtgccg
     4441 gtgcatgaac cgggctggcg acagcccgcc cagcagggtc aggggttgat cgatctgcat
     4501 tgggcaagca tacgcaagcc gtggccgacc cggtttgcgg tgcgacaatc gccccatgga
     4561 aatcaccccc caatgcgtgg tcgccctgac ctgggtgctg aaagacagcc tgggcgacac
     4621 cctggacgaa ctgcacgagc cggtcgagtt tctggtcggc agcgaagacc tgctgcccgt
     4681 cctcagcgcg gccctgcagg ggcatgagtc gggcgccacg ctgaacctga acctggagcc
     4741 ggaggacgcc ttcggcgact ttgatgagca actggtgttt ctggcccggc gcgaccagct
     4801 gccacccggc atcgaagaag gcatgctgat cgaggcagct tcgctgccgg ccggcagcgt
     4861 ggcgcaggcg ccggcggaca cgctgttcac catcaccgag atctaccccg agcacgtggt
     4921 gctggatgcc aaccatccgc tggccggcat ggcgttgcgg ctgcaactga aggtggtggc
     4981 ggtgcgcgaa gcattagacg atgagctgga gcgcggcagc gccggcgccg gcttctttcg
     5041 actgcaaccg atggcgccgg gcagcccgca cctgcactga acctgcgaca acggcaccct
     5101 gaggtgccat ggcgctgtct atgcccggac ccggtcagcg gtacagctga tcgccttcgg
     5161 cccgcacgcg atcgccgatc tgcacgttgg ggtgctgggc gtactcgaac tggcgcacgc
     5221 cgccgttgtc gaactgcacc gtgacgcggt aatgctcgac ccggttgcgg tccatgttgc
     5281 gctccagcat gttgccgatc agtgcgccgc cgatcacgcc cgccaccgtg gctgccgtgc
     5341 ggccgctgcc gccacccact tggctgcccg ccaggccacc cacggcgccg ccgatcactg
     5401 cgccggccac accctgcgac tggccgccgc gcacgtattc gacattcgtc acgcggccga
     5461 actgcgagta ggcctggttg cccggatagc tgcccggata gctgcctggg tagttgccct
     5521 gcgggtagcc cccgtgctgc gcgcaggcgg ccagcgtcag tgcggcggcg gcggtggcga
     5581 gcgaacggac gaatcgttga gatagatgca tggtggtact cctggaaaaa catgccctgg
     5641 cgcagcgcgg tgagtgcggc accagacacc ctggcattct ggcagagcgg tcacacccgc
     5701 gccaacacgc ccgccatcaa ccgcgaccgg tcgagccgta gccgcccgca ccccgctgcg
     5761 atgcctcgaa ggcgtcgacg atctgaaagc tggcctgcac caccggaacg atgaccagct
     5821 gggcgatgcg ctccatcggc tcgatggtga aggcgctggc gctgcggttc caggcgctga
     5881 ccatcagctg gccctggtag tcgctgtcga tcagtccgac caggttgccc agcacgatgc
     5941 cgtgcttgtg cccgagaccg gagcgcggca ggatcagcgc cgcgtagcca gggtcggcca
     6001 ggtgaatcgc catgcccgtg ggcaccagct gccaggcgcc ggccgccagg gtcagcggcg
     6061 cgtccaggca ggcacgcagg tccagcccgg cgctgcccgg cgtggcgtag gccggcatct
     6121 gcgtgcgcag acgctggtcg agaatgcgga cgtccagcct catgcgtcca gccccgagac
     6181 gcggcgcgcg atctcgtgga tcagctgctg cgccagatgg cgtttggagg cgcgcggcaa
     6241 ctccgtcacc ccttcgtcgt ccaccagcaa cagggcgttg tcgtccttgc cgaaggtggc
     6301 cgggccgagg ttggccacca gcagcggcac gcccttgcgc acgcgcttgg cctgggcgtt
     6361 ggcctgcagg tcctggcttt cggccgcgaa gccgacgcag tacagctcgc cggcctgggc
     6421 gcgcgccgaa tgggcgatgt cggccaggat gtcggggttt tcgacgaagc ccagctgcgg
     6481 cacctggccg gagccgtcct tcttgatctt gtgttcggcg gcgctggccg ggcgccagtc
     6541 cgccaccgcc gccgtagcta tgaaaatatg agcatgctgc gcatgtccgg tggccgccgc
     6601 gtgcatctgg cgtgcggagc cgacgtcgat gcggtgcacc ccgcgcgggg tcggcagatg
     6661 caccggcccg gccaccagcg tcacctcggc gccggcttcg cgcgccgcgc gggcaatcgc
     6721 gaaacccatc ttgccgctgg acaggttggt gatgccgcgc accgggtcga tggcctcgaa
     6781 cgtcggcccg gccgtgatca gcacgcgctg gcccagcagc gacttggggg ccagaaaggc
     6841 gatcagctcc tccagcagct cggccgcctc cagcatgcgg ccatcgccga actcgccgca
     6901 ggcctgcgcg ccgtgcgcca cgcccagcac ctgcgcgccg tcctgcgcca cctgccgcaa
     6961 gttgcgctgc gttgccggat gggcccacat ctcgcggttc atggccgggg cgagcagcag
     7021 cggcacctgc tcgatcggcc gcgccaggca cagcagcgcc agcagctcgc cggccctgcc
     7081 ctgcgccagc tgggcgatga agtcggcact ggccggcgcc accacgatgg cgtcggcctc
     7141 gcggctgagg ttgatgtgcg ccatggcgtt gtccggccgc gcgtcccact gcgacacgta
     7201 caccggccgg ccggacagcg cctgcatcgc caccggggtg atgaactggc aggccgcctc
     7261 ggtcatcacc acctgcaccg tggcgccggc cttggtcagc aggcggcagt attcggccgc
     7321 cttgaagcag gccgcgccac ccgacaggcc cagcacgatg tggcggccct gcagctcccc
     7381 cgcgaagtcc atggcattca tggcgcgcaa tgtagcagag ggtccggctc ctataattga
     7441 aatttcccac ccagcggagg ttgcggcctt cgcccctgac atgaccaaat tcgtcttcgt
     7501 cactggcggt gtggtgtctt ccctgggcaa gggaatcgcc tcggcctccc tggccgcgat
     7561 cctcgaatcg cgcggcctca aagtcaccct catcaagctc gatccgtaca tcaacgtcga
     7621 tccgggcacc atgagcccga tccagcacgg cgaggtgttc gtcaccgacg atggcgccga
     7681 gaccgacctg gacctgggcc actacgagcg cttcatcacc acccggatga agaaggccaa
     7741 caacttcacc accggccaga tctaccagag cgtgctcgag aaggaacggc gcggcgacta
     7801 cctgggcaag acggtacagg tgattccgca catcacgggc gagatccagg acttcatccg
     7861 gcgcggtgcc ggcgtcggca cgcccgacgc ggtggacgtg gccatcgtcg agatcggcgg
     7921 caccgtgggc gacatcgaat cgctgccttt ccttgaggcg gtgcgtcagc tcagcctgaa
     7981 gctggggccc aaccacagcg ccttcgtgca cctgacctac ctgccctgga tcgccgccgc
     8041 cggcgagctg aagaccaagc cgacccagca cacggtgcag aagctgcgcg agatcggcat
     8101 ccagcccgac gccctgctgt gccgcgccga ccgcaccatt ccgcaggacg agcgcgagaa
     8161 gatcagcctg ttcaccaacg tgccggcctg gggcgtcatc agcatgcccg acgtcgacac
     8221 catctacaaa gtgccgcgcc tgctgcatca gcagggcctc gacggcctga tctgcgacaa
     8281 gctgcgcctg aacacgccgc cggccaacct caagcgctgg gatgccctgg tgcacgaggt
     8341 cgaaagcccg cggcacgagg tctcgatcgc catggtcggc aaatacgtcg acctgtcgga
     8401 cagctacaaa tcggtcaacg aggcgctgcg ccacgccggc atgcacaacc atgcgcgcgt
     8461 gcacatcgag cacatcgatt ccgagaccat cacgcccgcc acggcggcgc agctggcgcg
     8521 cttcgacgcc atcctggtgc ccggcggctt tggcgagcgc ggggtcgaag gcaagatcgc
     8581 caccgtgcag tacgcgcgcg agcacaagct gccctacctg ggcatctgcc tgggcatgca
     8641 ggtggccacc atcgaatacg cgcgccacgt ggccggactg gaaggcgcca acagcaccga
     8701 gttcaaccct gccaccaggc atccggtcat cgcgctggtc accgaatgga aggacaagga
     8761 cggccgcatt cagcggcgcg acgccggctc cgacctgggc ggcaccatgc gcctgggcgc
     8821 gcagagcgcc gacgtggtgc ccggcacgct ggcgcaccgc atctacggcg acgtggtgac
     8881 cgagcgccat cgccaccgct acgaagccaa cgtggactac ctggagcgcc tgcgcgccgc
     8941 cggcctggtg atttcggcgc tcacgcagcg cgagcaactg accgagatcg tcgagctgcc
     9001 gcagggcgtg cacccgtggt tcgtcggcgt gcagttccac cccgagttca agtccacgcc
     9061 ctgggacggc cacccgctgt tcaacgcctt catcgaggcc gccctgcggc accaggccag
     9121 ccgccgcgcc gccgcgccca aggagacggc atgaagctgt gcggcttcga cgtcggcctg
     9181 cggcgtccgt tcttcctcat cgccggcccc tgcgtggtcg agtccgagca gttgcagatg
     9241 gacgtggccg gccagctgaa ggaaatcacc gccagcctgg gcatcccctt catcttcaag
     9301 agcagctacg acaaggccaa tcgctccagc ggtgccagct tccgcggccc gggcatggac
     9361 gccggcctgc agatcctggc ccgcgtgcgg cgcgagatcg gcgtgccggt gctcaccgac
     9421 gtgcacaccg aggccgaggt gcccaccgtg gccgccgtgg tggacgtgct gcagacaccc
     9481 gccttcctgt gccgtcagac cgatttcatc cgcgccgtgg cgcagtcggg caaaccggtc
     9541 aacatcaaga agggccagtt cctggcgccg cacgacatgg tcaacgtgat cgacaaggcg
     9601 cgtgcggcgg cggctgaaaa aggcctggac accgaccgct tcatggcctg cgagcgcggc
     9661 gccagctttg gctacaacaa cctggtgtcc gacatgcgca gcctggccat catgcgcgaa
     9721 accggtgcgc cggtggtgtt cgacgccacg cattcggtgc aactgccggg cggtctgggc
     9781 caggccagcg gcggccagcg cgagatggtg ccggtgctgg cgcgcgcggc ggtcgccgtg
     9841 ggcgtggccg gcctgttcat ggaaacccat cccgacccgg ccagggcgct gtccgacggg
     9901 cccaacgcgg tccctctcag gcacatgaaa gcgctgctgg agacgctggt ggcgctcgat
     9961 caggtcacca agcgccaggg ctttctggaa gacgacttcg gagcctgaac catgcccgcc
    10021 ggctacatca tcgccctggt caccatcacc aacccgcaac agtacgagga atacaagcgc
    10081 tggagcagcg aagccatgcg cgtgcacggc gctgaaatct gcgtgcgcgg agaccgggtg
    10141 gaggtgctgg agggcgactg gcacccgggg cgcacggtga tcctgaagtt ccccagcttc
    10201 gaggccgcca aggcctttca cgactcaccc gagtacagcc gcgcgcgcgc cgcccgccag
    10261 ggcgccgccg tcatgcgcct gctctgcgtc gagggcgtct gatgctatta gtttgatagc
    10321 tgctggcgct tgctggtatt gcgctagagc ccgattttta ttgaaacttt cggaaaagga
    10381 aaaccatgag tgccatcgtt gacatcgtcg gccgcgaagt gctggacagc cgcggcaacc
    10441 ccaccgtcga gtgcgacgtg ctgctggagt cgggcgtgat gggccgcgcc gccgtgccca
    10501 gcggcgcttc caccggctcg cgcgaagcca tcgagctgcg cgacggcgac aagaagcgct
    10561 acctgggcaa gggcgtgctc aaggccgtcg agcacatcaa caccgaaatc tccgaggccg
    10621 tgctgggcct ggacgcggcc gaacaggcct ttctggacaa gaccctgatc gatctggatg
    10681 gcaccgacaa caagtcccgc ctgggcgcca acgccctgct ggcggtgtcg atggcggtgg
    10741 cgcgcgcggc ggctgaagaa tccggcctgc cgctgtaccg ctacttcggc ggcatgaacg
    10801 gctgccagct ccccgtgccg atgatgaacg tcatcaacgg cggcgcacac gccaacaaca
    10861 acctggacct gcaggaattc atgatcatcc cggtcggcgc gcccagtttc cgcgaagcgc
    10921 tgcgctgggg cgccgaggtg ttccacgcgc tcaagaagat catccacgac aagg
//
LOCUS       DAOOOG010000081        10958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6748_length_10958_cov_27.861598, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000081 DAOOOG010000000
VERSION     DAOOOG010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10958
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6748_length_10958_cov_27.861598"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PK242_07495"
     CDS             complement(<1..203)
                     /locus_tag="PK242_07495"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715643.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HOZ93898.1"
                     /translation="MSTAASLNDFLRRIEQRDPQQPEFLQAVREVMMSLWPFLEKHPR
                     YREHGLLERLCEPDRVIQFRVAWV"
     gene            complement(387..1166)
                     /gene="hisF"
                     /locus_tag="PK242_07500"
     CDS             complement(387..1166)
                     /gene="hisF"
                     /locus_tag="PK242_07500"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOZ93899.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGA
                     DELTFLDITATSDGRDLILPIIEAVASQVFIPLTVGGGVRTVDDVRRLLNAGADKVSF
                     NSAAIANPQVIRDASGKYGAQCIVVAIDARRRSGDELAARGPGWDVFSHGGRRNTGLD
                     AIAWARQMADWGAGEILLTSMDRDGTRSGFDLELTRAVSDAVPVPVIASGGVGSLEHL
                     ADGVQLGGADAVLAASIFHYGEFTVAQAKQHLAARGIPVRL"
     gene            complement(1176..1916)
                     /gene="hisA"
                     /locus_tag="PK242_07505"
     CDS             complement(1176..1916)
                     /gene="hisA"
                     /locus_tag="PK242_07505"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOZ93900.1"
                     /translation="MLLIPAIDLKDGHCVRLKQGDMDQATVFSEAPAEMAAHWLGQGA
                     RRLHLVDLNGAFAGKPQNLAAIKAILRQVDDEIPVQLGGGIRDLDTIERYIDAGIRYV
                     IIGTAAVKNPGFLKDACTAFGGHIIVGLDAKDGKVATDGWSKLTGHEVIDLARKFQDW
                     GVESIIYTDIGRDGMLSGINIEATVKLAQALTIPVIASGGLSTMADIEQLCAVESEGI
                     EGVIAGRAIYSGDLDFAAAQARADALNG"
     gene            complement(2012..2683)
                     /gene="hisH"
                     /locus_tag="PK242_07510"
     CDS             complement(2012..2683)
                     /gene="hisH"
                     /locus_tag="PK242_07510"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOZ93901.1"
                     /translation="MLSIANQTVAVVDYGMGNLRSVAQAVMHAAQDTRVEVVVTDDPA
                     RVRAAHRVVLPGQGAMGDCMHQLHACGLLESVLEAADGKPLFGVCIGMQMLLTHSAEG
                     HTAAGTPGLGLIPGEVLRFDLAGRQAPDGSRYKVPHMGWNRVFQSRPHPLWQGVADGA
                     WFYFVHSYFARPHTPTHAVGETEYGARFASAVARDNIFATQFHPEKSADQGLTLYRNF
                     LNWRP"
     gene            complement(2727..3326)
                     /gene="hisB"
                     /locus_tag="PK242_07515"
     CDS             complement(2727..3326)
                     /gene="hisB"
                     /locus_tag="PK242_07515"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOZ93902.1"
                     /translation="MTTTARSADVARDTAETRIRVRLNLDGSGQATLATGIGFFDHML
                     DQIARHGLIDLDVQCQGDLHIDGHHTVEDVGIAIGQAVRQAVGDKRGITRYGHSYVPL
                     DEALSRVVIDFSGRPGLVLDARFASAMIGGFDTQLVHEFFQGFVNHAQVTLHIDNLKG
                     VNAHHQVETIFKAFGRALRMALTPDARAAGQVPSTKGTL"
     gene            complement(3323..4426)
                     /gene="hisC"
                     /locus_tag="PK242_07520"
     CDS             complement(3323..4426)
                     /gene="hisC"
                     /locus_tag="PK242_07520"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOZ93903.1"
                     /translation="MSTDTSTPSPLARIRQDVQSMHAYAVQDSAGLLKLDAMENPHRL
                     PDALQAELGRRLGRVAINRYPGARVAELGAALHAHAAPPAGWSLMLGNGSDELISLLA
                     MACDVPGASILAPLPGFVMYAMSARLQGLAFHGVPLTPDFALDEAAMLAAIERHRPAI
                     LYLAYPNNPTGTLWDAGSIARLIAAQGRQGGLVVMDEAYQPFAAHSWIEVVRAAPEPH
                     RHVLIMRTLSKFGLAGVRIGYLMGEATLLAEIDKVRPPYNVSVLNAECALFALEHADV
                     FAAQAADIRAQRAQLLQALAALPGVTPFPSQANMVLARVPDAARVFAGMRARGVLIKN
                     VSTMHALLANCVRLTVGTADENAQMLAALQASL"
     gene            complement(4416..4874)
                     /locus_tag="PK242_07525"
     CDS             complement(4416..4874)
                     /locus_tag="PK242_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOZ93904.1"
                     /translation="MMPPLVFVDTPVFLYAADERDGRKQALAREWVRWCWSQRSGRIS
                     TQVLNELYHNAITRFRARMPVQQARAQVRNLRQWQPPHLDTFTIDGAWALQDRHALGY
                     WDALIVASAQQQGCQYLLCEDLPHQQRFESVQVINPFRVRPDELPLADEH"
     gene            complement(4871..5089)
                     /locus_tag="PK242_07530"
     CDS             complement(4871..5089)
                     /locus_tag="PK242_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOZ93905.1"
                     /translation="MKNVTITVDEAVLEWARVEAARRGSSVSRMVGEMLAEKMRQEDA
                     YAQAMRSALRFESWGASDGPYLRLSEVE"
     gene            5197..5769
                     /locus_tag="PK242_07535"
     CDS             5197..5769
                     /locus_tag="PK242_07535"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOZ93906.1"
                     /translation="MRARTLALLGVGAYALYRLSQQTGLPPGVHPVTPFRLERWLGRW
                     YEIARIDPPQDRGLTDGVVHHSLLPDGTLQIARRGFDTRLGRWRTTTARARPAHPSRE
                     AHLHLSLVWPVRTSRAVFDIDDADQHALVCGPTHDQLWLLARSPTMRAATRARLLDRA
                     RAAGFDVGRLTWVDHRRSVAVVGRGSSSTH"
     gene            complement(5808..7076)
                     /gene="gltS"
                     /locus_tag="PK242_07540"
     CDS             complement(5808..7076)
                     /gene="gltS"
                     /locus_tag="PK242_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HOZ93907.1"
                     /translation="MEITLNGYHTLILAALVMLVGHWLVFRIRLLSAFNIPEPVAGGL
                     LAAAIVYALNVALGVRFKFSTELQTSFMLMFFSSIGLSADFTRLKEGGKPLVVLLVIV
                     SCFIVVQNLIGVGLAAALGLKPLLGLVVGSITLTGGHGTAGAWGPVLEQNYGIAGATT
                     LGIATATFGLVFGGLIGGPVARYLIRQQHRQPMPEAGVRDSIDTISGKPRHGETDSSV
                     RDRRLTFDMLDEPRLITAYSAIETLAMFAACLTAADLVSGWTKGSAIELPTFIWALAS
                     GVVIRNVLTHAFRFEMFDRAIDVFGNASLSLFLAMALLSLRLWELADLAGPVAIILAV
                     QVLVMMAWASFVTYRFMGADYDAAVVAAGHCGFGLGATPTAIANMQAITDRFGPSYKA
                     YLVVPLVGAFFVDLVNATVLQIFTQLPFLR"
     gene            complement(7198..8529)
                     /gene="hisD"
                     /locus_tag="PK242_07545"
     CDS             complement(7198..8529)
                     /gene="hisD"
                     /locus_tag="PK242_07545"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOZ93908.1"
                     /translation="MKFIAAPARLSTLDADFEQQFQQRLHWSAEQDQAIEQRVRDILA
                     DVRARGDAALLDCTARFDGVQAASVAALELRAAELKAAFDGLPAEQREALTQAAARIR
                     RYHEWQKQQGGETATYRDEDGTLLGQKVTPLDRVGIYVPGGKAAYPSSVLMNAIPAHV
                     AGVGEIVMVVPTPRGERNPLVLAAAHVAGVTRAFTLGGAQAVAALAYGTATVPRVDKI
                     TGPGNAYVASAKKHVFGQVGIDMIAGPSEILVLADGSTPADWVAMDLFSQAEHDELAQ
                     SILLSPDAAYIDAVQAAIERLLPTMPRAEIIARSLTGRGALIHTRSMEEACDIANRIA
                     PEHLEVSSREPQRWEPRLRHAGAIFLGAWTSESLGDYCAGPNHVLPTSGTARFSSPLS
                     VYDFQKRSSLIEVSAEGARRLGRIASVLARGEGLQAHARAAELRDPAAATR"
     gene            complement(8531..9175)
                     /gene="hisG"
                     /locus_tag="PK242_07550"
     CDS             complement(8531..9175)
                     /gene="hisG"
                     /locus_tag="PK242_07550"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOZ93909.1"
                     /translation="MITLALSKGRIFDETLPLLAAAGIEVLEDPETSRKLILPTNQDG
                     VRVVLVRATDVPTYVEYGGADLGVTGKDVLIEHGGAGLYQPLDLRIAQCHMAVAAPEG
                     FDYQRAVRQGSRITVATKYTTIARNFFADKGVHVDLIKLYGSMELAPLTGMADAIVDL
                     VSTGKTLKANRLVEVEHIMDISSRLVVNQAALKLKTAPIRRIIDAFAQAIGPAI"
     gene            complement(9292..10566)
                     /gene="murA"
                     /locus_tag="PK242_07555"
     CDS             complement(9292..10566)
                     /gene="murA"
                     /locus_tag="PK242_07555"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOZ93910.1"
                     /translation="MDKLLIRGGRSLQGEVLISGAKNAALPEMCATLLSSAPVHLVNV
                     PRLHDVRTMRHLLDHMGVATQTHGERGGMSFVAPDDLNPEAPYELVKTMRAAVLVLGP
                     LLARFGRARVSLPGGCAIGSRPVDQHIKGLQAMGAQIEVEHGYMVARLPAGRSRLRGA
                     RITTDMVTVTGTENLLMAAALAEGETVLENAAQEPEITDLAELLIGMGARIEGHGTSR
                     IRIEGVERLHGATHEVVADRIEAGTFLCAVAATGGDVTLRHARADHLDAVIDKLRDAG
                     VQVEALDGAIRVRSAGGPALRAQSFRTIEYPGFPTDMQAQFMVLNCVAAGPSMVTETI
                     FENRFMHVNELARLGAQIHVDGRNAMIEGAARLSGATVMATDLRASASLVIAGLIAEG
                     ETVVDRIYHLDRGYDQMEEKLRALGADIERVR"
     gene            complement(10642..10884)
                     /locus_tag="PK242_07560"
     CDS             complement(10642..10884)
                     /locus_tag="PK242_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HOZ93911.1"
                     /translation="MNAEQIKQLIAAGLVCEHLELEGDGRHWFAVIVSPQFEGKRPIA
                     RHQMVYATLGSQLQTDAVHALSMKTLTPAEWAAQRS"
BASE COUNT         1674 a         3721 c         3838 g         1725 t
ORIGIN      
        1 acccaggcga cgcggaactg gatcacgcgg tcgggctcgc acaggcgctc cagcaagccg
       61 tgttcgcggt accgggggtg tttctccagg aacggccaga gcgacatcat gacctcgcgc
      121 accgcctgca ggaactctgg ttgctggggg tcgcgctgtt cgatacggcg cagaaagtcg
      181 ttcaggctcg cggcggtgct caacatgggc tccttggatg ggatgggtgg gtgcgtcgaa
      241 ccggcgttcg atcgcggccc gcgtccgcct tgggcatgcc ggggcatgcg cggaccgtgg
      301 gccgtggatc ataacggctg ccagcggcgg tttcctgacg cacggcaaag gtgttcatcg
      361 gcggtgttcg caagcgcccg cgggcgtcac aggcgcaccg gaatgccgcg cgccgccagg
      421 tgctgcttgg cctgggcgac ggtgaattcg ccgtagtgaa agatgctggc cgccagcacg
      481 gcgtcggcgc cgccgagctg cacgccgtcg gccaggtgtt cgaggctgcc gacgccgccg
      541 gaggcgatca ccggcaccgg caccgcgtcg ctcaccgcgc gggtcagttc caggtcgaag
      601 ccgcttctgg tgccgtcgcg gtccatgctg gtgagcagga tttcgccggc gccccagtcg
      661 gccatctggc gtgcccaggc gatggcgtcc agcccggtgt tcctgcgccc gccgtggctg
      721 aacacgtccc agccgggacc acgcgcggcc agctcgtcgc cgctgcgccg gcgcgcgtcg
      781 atggcgacca cgatgcactg cgcgccgtac ttgcccgagg cgtcgcggat cacctgcggg
      841 ttggcgatgg cggccgagtt gaagctgacc ttgtcggcgc cggcgttgag caggcggcgc
      901 acgtcgtcca ccgtgcgcac gccgccgccg acggtgagcg gaatgaacac ctggctggcc
      961 accgcttcga tgatcggcag gatcaggtcg cgcccgtcgc tggtggcggt gatgtcgagg
     1021 aaggtgagct cgtcggcgcc ctgctcgttg tagcgcgcgg cgatctcgac cgggtcgccg
     1081 gcatcgcgca gttcgacgaa attgacgccc ttgacgacgc gaccgccggt cacgtccagg
     1141 cagggaatga tgcgcttggc cagcatggaa tggggtcagc cgttcagcgc gtcggcgcgc
     1201 gcctgcgcgg ccgcgaagtc gagatcgccg gaatagatgg cacgaccggc gatgacgcct
     1261 tcgatgcctt cgctctcgac cgcgcacagc tgctcgatgt cggccatggt ggacagcccg
     1321 ccggaggcga tgaccgggat ggtgagcgcc tgcgccagct tgacggtggc ttcgatgttg
     1381 atgccgctga gcatgccgtc gcggccgatg tcggtgtaga tgatgctttc cacgccccag
     1441 tcctggaact tgcgcgccag gtcgatcacc tcgtggccgg tgagcttgct ccagccgtcg
     1501 gtggcgacct tgccgtcctt ggcgtccaga ccgacgatga tgtggccacc gaaggcggtg
     1561 caggcgtcct tcagaaagcc cgggttcttg accgccgccg tgccgatgat gacgtagcgg
     1621 atgccggcgt cgatgtagcg ctcgatggtg tcgaggtcgc ggatgccccc gcccagctgc
     1681 accgggattt cgtcatcgac ctgccgcagg atcgccttga tggccgccag gttctgcggc
     1741 ttgccggcaa aggcgccgtt caggtccacc agatgcaggc ggcgcgcgcc ctggcccagc
     1801 cagtgcgccg ccatctcggc cggggcctcg ctgaaaaccg tggcctggtc catgtcgccc
     1861 tgcttcaggc gcacgcaatg gccgtccttg agatcgatgg cggggatcag caacatgttc
     1921 gggacagatg gaacgaatga acgatgaaga agtcgatcgg ccgggctggg cggtgcgtgc
     1981 agaacgcgtc gcgcacaacg ccgcgccagg ttcagggccg ccagttcagg aagttgcggt
     2041 acagggtaag gccctggtcg gcactctttt ccgggtggaa ctgggtagca aaaatattat
     2101 cgcgcgccac ggcgctggca aaacgtgcgc cgtactcggt ttcgcccacg gcatgggtcg
     2161 gcgtgtgcgg gcgggcaaag tagctgtgca cgaaatagaa ccaggcgccg tcggccacgc
     2221 cctgccacag cgggtgcggg cggctctgga acacccggtt ccagcccatg tgcggcacct
     2281 tgtagcggct gccgtcgggc gcctggcgac cggccagatc gaagcgcagc acctcgcccg
     2341 ggatcaggcc cagaccgggt gtgccggcgg cggtgtggcc ctcggcgctg tgcgtcagca
     2401 gcatctgcat gccgatgcag acgccgaaca gcggcttgcc gtcggcggcc tccagcaccg
     2461 attcgagcag gccgcaggca tgcagctggt gcatgcagtc gcccatcgcg ccctgccccg
     2521 gcagcaccac gcggtgcgcc gcgcgcacgc gcgccggatc gtcggtgacc accacctcca
     2581 cgcgcgtgtc ctgcgcggcg tgcatcaccg cctgcgccac cgagcgcagg ttgcccatgc
     2641 cgtagtcgac cacggcgacc gtttgatttg ctatcgataa catagctgtt ggcgcttatc
     2701 tgtcaagcgc tggaggccga tttgacttag agcgtgccct tggtcgaggg cacctgaccg
     2761 gcggcgcgcg catccggcgt gagcgccatg cgcagcgcac ggccaaaggc cttgaagatg
     2821 gtttcgacct ggtggtgcgc gttcacgccc ttcaggttgt cgatgtgcag cgtgacctgg
     2881 gcgtggttga cgaagccctg aaagaattca tgcaccagtt gggtgtcgaa gccgccgatc
     2941 atggcgctgg caaagcgcgc gtccagcacc aggccggggc gacccgaaaa atcgatcacc
     3001 acgcgcgaca gcgcttcgtc cagcgggacg tagctgtggc cgtagcgggt gatgccgcgc
     3061 ttgtcgccca ccgcctggcg caccgcctgg ccgatggcga tgcccacgtc ctccaccgtg
     3121 tggtggccgt cgatgtgcag atcgccctgg cactgcacgt ccagatcgat caggccgtgg
     3181 cgcgcgatct ggtcgagcat gtggtcgaaa aaaccgatgc cggtggccag cgtggcctgg
     3241 ccgctgccgt ccaggttcag gcgcacacgg atgcgggttt cggcggtgtc gcgcgcgacg
     3301 tcggcgctgc gtgcagtggt ggtcatagcg aggcttgcag ggccgccagc atctgcgcgt
     3361 tctcgtcggc ggtaccgacc gtcaggcgca cgcagttcgc cagcagcgcg tgcattgtag
     3421 aaacgttctt gatcagcaca ccgcgcgcgc gcatgccggc aaacacgcgc gccgcatcgg
     3481 gcacgcgcgc cagcaccatg ttggcctggc tgggaaacgg cgtgacaccc ggcagcgccg
     3541 ccagcgcctg cagcagctgc gcgcgctgcg cgcggatgtc ggccgcctgc gcggcaaaca
     3601 cgtcggcgtg ttcgagcgcg aacagcgcgc actcggcgtt gagcacgctg acgttgtagg
     3661 gcgggcgcac cttgtcgatt tcggccagca gcgtcgcctc gcccatcagg tagccgatgc
     3721 gcacgccggc cagcccgaac ttggacagcg tgcgcatgat cagcacgtgg cggtgcggct
     3781 cgggggcggc gcgcaccacc tcgatccagc tgtgcgcggc aaagggctgg taggcctcgt
     3841 ccatcaccac caacccgccc tggcggccct gggcggcaat cagacgggcg atgctgccgg
     3901 cgtcccacaa ggtgccggtg gggttgttgg ggtaggccag gtacaggatg gccgggcggt
     3961 ggcgctcgat ggcggccagc atggcggcct cgtcgagcgc gaagtcgggc gtcagcggca
     4021 caccgtgaaa cgccaggccc tgcaggcgcg cactcatggc atacatcacg aagccgggca
     4081 gcggcgccag gatagacgct cccggcacgt cgcaggccat ggccagcagg ctgatcagct
     4141 cgtccgagcc gttgcccagc atcagcgacc aacccgccgg cggcgcagcg tgggcgtgca
     4201 gcgcggcgcc cagctcggcc acgcgggcgc cgggatagcg gttgatggcc acccggccca
     4261 ggcggcgacc cagctcggcc tgcagggcgt caggcaggcg atgggggttt tccatcgcgt
     4321 ccagtttcag caggccggcc gagtcctgca ccgcgtaggc gtgcatggac tgcacgtcct
     4381 ggcggatacg ggccagcggg ctgggggtcg aggtgtcagt gctcatcggc aaggggcagt
     4441 tcgtcgggac ggacccggaa ggggttgatc acctgcacgc tctcgaagcg ctgctgatgc
     4501 ggcaggtcct cgcacagcag gtactgacag ccttgctgtt gggccgaggc gacgatcagc
     4561 gcatcccagt agcccagcgc atggcgatcc tgcagcgccc aggcgccgtc gatggtgaag
     4621 gtatccaggt gcggcggctg ccactgacgc aggttgcgca cctgcgcgcg cgcctgctgc
     4681 accggcatgc gggcgcgaaa gcgcgtgatg gcgttgtggt agagctcgtt gagcacctgg
     4741 gtgctgatgc ggccgctgcg ctggctccag caccagcgca cccattcgcg cgccagcgcc
     4801 tgcttgcgcc catcgcgctc gtcggctgcg tacaggaaca ccggcgtgtc gacgaacacc
     4861 aggggcggca tcattcgacc tcgctcagcc gcaggtacgg cccgtcgctg gcgccccagg
     4921 actcgaagcg cagcgcgctg cgcatggcct gggcgtaggc gtcttcctga cgcatcttct
     4981 cggccagcat ctcacccacc atgcgcgaaa cgctggagcc gcgccgcgcc gcctccaccc
     5041 gcgcccattc gagcacggct tcgtcgacgg tgatggtgac gttcttcatg tcgggcagtg
     5101 tacacgaatt tcgtgcaaca cgaacttcgt gtcatccgca ccggtgccgc cgcacgcccg
     5161 ccgcagccgc gcgcctgcta tgctgccgcg ctgaccatga gagccagaac ccttgccctg
     5221 ctcggggtcg gcgcctatgc gctgtaccgc ctgagccagc agaccggtct gccgcccggc
     5281 gtgcacccgg tgacgccgtt caggctggaa cgctggctcg gccgctggta cgaaatcgcg
     5341 cgcatcgacc cgccgcagga ccgtgggctg accgacggtg tggtgcacca cagcctgctg
     5401 cccgatggca cgctgcagat cgcccggcgc ggcttcgaca cgcgcctggg gcgctggcgc
     5461 accaccacgg cacgcgcgcg cccggcgcac ccgtcgcgcg aggcgcacct gcacctcagc
     5521 ctggtctggc cggtgcgcac cagccgcgcc gtgttcgaca tcgacgacgc cgaccagcac
     5581 gccctggtct gcggccccac gcacgaccag ctgtggctgc tggcgcgcag cccgacgatg
     5641 cgtgccgcca cgcgggcccg cctgctcgat cgcgcgcgtg ccgccggctt cgacgtcggg
     5701 cgcctgacct gggtcgatca ccgccgcagc gtggccgtgg tcggccgtgg ctcgtccagc
     5761 acccattgaa gccagcaagg gggcgccttg catggcgtga accggcccta acgcagaaac
     5821 ggcaactgcg tgaagatttg cagcaccgtg gcgttcacca ggtcgacgaa gaaggccccc
     5881 accagcggca ccaccagata ggccttgtac gacgggccaa agcgatcggt gatggcctgc
     5941 atgttggcga tcgccgtcgg cgtggcgccc aggccgaagc cgcagtggcc ggccgccacc
     6001 actgcggcgt cgtagtcggc ccccatgaag cggtaggtca cgaacgacgc ccaggccatc
     6061 atcaccagca cctgcaccgc caggatgatg gccaccggac cggccagatc ggccagctcc
     6121 cacaggcgca ggctgagcaa ggccatggcc agaaacagcg acagcgaggc gttgccgaac
     6181 acgtcgatgg cgcggtcgaa catctcgaag cggaaggcat gggtcagcac gttgcgaatc
     6241 accacgccac tggccagcgc ccagatgaag gtgggcagct cgatggcgct gcccttggtc
     6301 cagccactca ccaggtcggc cgcggtcagg caggcggcaa acatggccag tgtctcgatg
     6361 gcgctgtagg cggtgatcag gcgcggctcg tcgagcatgt cgaaggtcag gcgcctgtca
     6421 cggacgctgc tgtcggtttc gccatgacgc ggtttgccgg agatggtatc gatcgagtcg
     6481 cgcacaccgg cctccggcat gggctgcctg tgctgctggc ggatcaggta gcgcgccacc
     6541 ggaccgccga tcagcccgcc gaacaccagg ccaaaggtgg ccgtggcgat gcccagcgtg
     6601 gtggcgccgg cgatgccgta gttctgctcc agcaccggac cccaggcccc cgcggtgccg
     6661 tggccgccgg tgagcgtgat ggagccgacc accaggccga gcagcggttt cagtcccagc
     6721 gccgcagcca gaccgacgcc gatcaggttc tgcaccacga taaagcagga gacgatgacc
     6781 agcagcacca ccaggggctt gccgccctcc ttcagtcgcg tgaaatcggc gctcagcccg
     6841 atggacgaga agaacatcag catgaagctg gtctgcagtt cggtgctgaa cttgaaccgg
     6901 acgcccagcg cgacgttcag ggcatagacg atcgccgccg ccagcagccc tccagccaca
     6961 ggctccggga tgttgaaggc cgacagcaac cggatgcgaa acaccagcca gtgcccgacc
     7021 agcatgacca gtgccgccag gatcagggtg tggtagccgt tcagcgtgat ttccaccgtg
     7081 cgctcctctc cggcccacag gccgcccgcc gccgcggcac ctgcctgggc ggcacgccgc
     7141 tgcatgcaac gcatgatgcc accaccgcgc gcggcggtcg atggccggtg gggcctgtca
     7201 gcgtgtcgct gccgccgggt cgcgcaactc ggccgcgcgc gcgtgcgcct gcaggccctc
     7261 gccgcgcgcc agcacgctgg cgatgcggcc cagccggcgc gcgccttcgg cgctgacctc
     7321 gatcaggctg ctgcgcttct ggaagtcgta caccgacagc ggcgacgaaa agcgcgccgt
     7381 gccgctggtg ggcagcacgt ggttggggcc ggcgcagtag tcgcccagac tctcgctggt
     7441 ccaggccccc agaaagatgg cgccggcgtg gcgcagccgc ggctcccagc gctgcggctc
     7501 gcggctgctg acttccaggt gctcgggcgc gatgcggttg gcgatgtcgc aggcctcttc
     7561 catgctgcgg gtgtggatca gcgcaccgcg cccggtcagg ctcctggcga tgatctcggc
     7621 gcgcggcatg gtgggcagca ggcgctcgat ggccgcctgc acggcgtcga tataggcggc
     7681 gtcggggctg agcaggatgc tttgtgccag ctcgtcgtgt tcggcctggc tgaacaggtc
     7741 catcgccacc cagtcggccg gcgtgctgcc gtcggccagc accaggattt cgctcgggcc
     7801 ggcgatcatg tcgatgccga cctggccgaa cacgtgcttc ttggcgctgg ccacataggc
     7861 gttgccgggg ccggtgatct tgtccacccg aggcacggtg gccgtgccat aggccagcgc
     7921 cgccacggcc tgcgcgccgc ccagcgtgaa ggcacgcgtg acgccggcca cgtgcgcggc
     7981 ggccagcacc agcgggttcc tctcgccacg cggggtcggc accaccatga cgatttcgcc
     8041 cacgccggcc acatgggccg ggatggcgtt catcagcacg ctgctggggt aggccgcctt
     8101 gccgccgggc acgtagatgc ccacgcggtc cagcggtgtc accttctgac ccagcagggt
     8161 gccgtcttcg tcgcggtagg tggcggtttc gccgccctgc tgcttctgcc attcgtggta
     8221 acggcggatg cgcgcggcag cctgcgtcag cgcctcgcgc tgctcggccg gcaggccgtc
     8281 gaaggcggct ttcagctcgg cggcgcgcaa ttccagcgcc gccaccgagg ccgcctgcac
     8341 gccatcgaag cgcgcggtgc agtccagcag cgccgcgtcg ccgcgcgcgc gcacgtcggc
     8401 caggatgtcg cgcacgcgct gctcgatggc ctggtcctgc tcggcgctcc agtgcagacg
     8461 ctgctgaaat tgttgctcga aatcggcatc cagcgtggac agacgtgcgg gagcagctat
     8521 gaatttcata tcaaatggcg gggccgatgg cctgggcaaa agcgtcgatg atgcgccgga
     8581 tcggcgccgt cttgagcttg agcgcggcct ggttgaccac caggcgcgag ctgatgtcca
     8641 tgatgtgctc gacctcgacc agccggttgg ccttgagcgt cttgccggtg ctgaccagat
     8701 cgacgatggc gtcggccatg ccggtcagcg gcgccagttc catgctgccg tagagcttga
     8761 tcaggtccac atgcacgccc ttgtcggcaa aaaagttgcg cgcgatggtg gtgtacttgg
     8821 tggccacggt gatgcggctg ccctggcgca cggcgcgctg gtagtcgaag ccctcgggcg
     8881 cggccacggc catgtggcac tgcgcgatgc gcaggtccag cggctggtac aggccggcgc
     8941 cgccgtgctc gatcagcacg tccttgccgg tcacgcccag gtcggcgccg ccgtactcga
     9001 cgtaggtcgg cacgtcggtg gcgcgcacca gcaccacgcg cacgccgtcc tggttggtgg
     9061 gcaggatcag cttgcgcgag gtttcgggat cctccagcac ctcgatgccg gcggcggcca
     9121 gcagcggcag ggtctcgtcg aagatgcggc ctttggacag ggcaagggtg atcattcagg
     9181 actcgcagac tggccgtacg cgcagggcgc gaaacctaca gccgaattga gggatcgatg
     9241 ccgcgccttg cgcgtctcct gcgggtgctg cgcgtccggt aggcatgggg ctcagcgcac
     9301 gcgctcgatg tcggcgccca gggcgcgcag cttttcttcc atctgatcgt aaccgcggtc
     9361 caggtgatag atgcgatcga ccacggtctc tccctcggcg atcagaccgg cgatcaccag
     9421 gctggccgag gcgcgcagat cggtcgccat cacggtggcg ccggacaggc gcgccgcgcc
     9481 ctcgatcatg gcgttgcggc catcgacgtg aatctgcgcc cccaggcgcg ccagctcgtt
     9541 gacgtgcatg aagcggtttt caaagatggt ctcggtgacc atgctggggc cggccgccac
     9601 acagttgagg accatgaact gcgcctgcat gtcggtcgga aagcccgggt attcgatggt
     9661 gcgaaagctc tgcgcgcgca acgccggccc gcccgcgctg cgcacgcgga tggcgccgtc
     9721 cagcgcctcg acctgcacgc cggcgtcgcg cagtttgtcg atcaccgcgt ccaggtggtc
     9781 ggcacgcgca tggcgcagcg tgacgtcacc gccggtggcg gcgaccgcgc acagaaaggt
     9841 gccggcctcg atgcggtcgg ccaccacctc gtgcgtggcg ccgtgcaggc gctcgacgcc
     9901 ctcgatgcga atgcggctcg tgccatggcc ctcgatgcgc gcgcccatgc cgatcagcag
     9961 ctcggccagg tcggtgattt cgggttcctg ggcggcgttt tccagcaccg tctcgccttc
    10021 ggccagcgca gcggccatga ggaggttctc ggttccggtg acggtcacca tgtcggtggt
    10081 gatgcgcgcg ccgcgcaggc ggctgcggcc ggccggcagg cgcgccacca tgtagccgtg
    10141 ctcgacctcg atctgcgcgc ccatggcctg caggcccttg atgtgctggt ccaccggccg
    10201 cgagccgatg gcgcagccac ccggcagcga cacccgcgcg cggccaaagc gcgccagcag
    10261 cgggcccagc accagcaccg ccgcgcgcat ggtcttgacc agctcgtacg gcgcctcggg
    10321 gttgaggtca tcgggagcga cgaagctcat gccgccgcgc tcgccgtggg tctgcgtggc
    10381 cacacccatg tggtcgagca ggtggcgcat ggtgcgcacg tcgtgcagac gcggcacgtt
    10441 gaccagatgc accggcgcgc tgctgagcag cgtggcgcac atctcgggca gcgcggcgtt
    10501 cttggcgccg gagatcagca cctcaccctg cagcgaacgg ccaccgcgga tcaacagttt
    10561 gtccatgctc aagcgaatga atgaagaaac agggctctgg ggcgctcctg acaagcgcca
    10621 caagctatca ataccatagt atcaggaacg ctgcgcggcc cattcggcgg gggtcagggt
    10681 cttcatcgac agcgcgtgca cggcgtccgt ctgcagctgg ctgcccagcg tcgcatacac
    10741 catctggtga cgtgcgatcg gccgcttgcc ctcgaactgg ggcgagacga tgacggcgaa
    10801 ccagtggcga ccgtcgccct ccagttccag gtgttcgcac accaggccgg cggcgatgag
    10861 ttgcttgatc tgttctgcgt tcatggcggg atgggttgaa ggggtcagta gcgcagcttg
    10921 tagccggtgc gcagcaggtg cagcgtcagc gccgacac
//
LOCUS       DAOOOG010000082        10712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_6994_length_10712_cov_29.391386, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000082 DAOOOG010000000
VERSION     DAOOOG010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6994_length_10712_cov_29.391386"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..781)
                     /locus_tag="PK242_07565"
     CDS             complement(74..781)
                     /locus_tag="PK242_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ93912.1"
                     /translation="MANILVVDDELGIRDLLSEILNDEGHTVELAENAAQARAARQNG
                     RPDLVLLDIWMPDTDGVTLLKEWRGAGLLTMPVIMMSGHATIDTAVEATRIGAQSFLE
                     KPITLQKLLKTVDHALSRDVPARRAVAEVVEAGVVVHAPADLTVEAAMTGGQSLPAAL
                     DMSPQGQQTFDLDKPLRDARDAFEKAYFEFHLAKEGGSMTRVAEKTGLERTHLYRKLK
                     QLGVDLSRNRRGAAVAL"
     gene            complement(812..3121)
                     /locus_tag="PK242_07570"
     CDS             complement(812..3121)
                     /locus_tag="PK242_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ93913.1"
                     /translation="MSTAAAAADPAQSARRRRSRWALWAAGTLLVGIGLVLLFLLAQA
                     TNNRLLYERYYTRLFIVNVVVAAVLGLVILWGVYRLLMRLRRGKFGSRLLIKLAAIFG
                     LVGVVPGLLIYVVSYQFVSRSIESWFDVRVEGALDSGLTLARTSLDTQAREFGNQLRT
                     ASVALADVPDALAALPLEQLRVQLGASDLTLWSASGRLLASAGRSRFQLTPERPSGAM
                     LRNLRAQGVATQIEGLDDVVPGGTTPEARVRALVAVSARTIGLVGEQRLLQAVQPLPT
                     SLVTHALAVQEAHREYQERALGREGLRRMYIGTLTLSLFLAVFGAVLLAVALGKQLAT
                     PLLMLADGMRQVATGDLTPKVILPGRDELVGLTRSFADMTQQLADARAALDASMHAVD
                     ASRARLQTILDNLTAGVIVLDAQGLIQSSNPGATRILRVPLAASAGRPLGSLEGLQAF
                     AAEVQTQFASFEGERGEHGLENWQKSFELYGHPGGGVATDALTLIARGAPLPDGLRLL
                     VFDDISEIVSAQRAHAWGEVARRLAHEIKNPLTPIQLSAERLEHKLADKLPEAERALL
                     VRAVRTIVDQVDAMKRLVNEFRDYARLPMADLKPVDLNALVTEVLQLYGGAGDVQHRQ
                     AVPVHFESDPHCPPILGDAQQLRQVIHNLVQNAIDASEGVVRDGPQAQAQGVIVRTQW
                     QDNLQRVRLAVIDSGHGFAEAILKRAFEPYVTTKPKGTGLGLAVVRKIADEHGARIDV
                     SNRINDGQILGAQVSLSFQPASEGRTALT"
     gene            complement(3118..3741)
                     /locus_tag="PK242_07575"
     CDS             complement(3118..3741)
                     /locus_tag="PK242_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOZ93914.1"
                     /translation="MTGFITRCCASVWISRLLLAWALACGLGAVARAEGAALSALSVA
                     REGNELILSARLDFALSPVVEDALHKGIPVHFVAEAEVLRERWYWADRSVALARRYLR
                     LSYQPLTRRWRLNTSSEPLTNAGLGVSLTQSFDTLDEALTAVQRIGRWRIASAADIDD
                     GGRHLLRFRFRLDASQLPRTLQIGSVGHSDWVISVERRIDLTQELAQ"
     gene            complement(3698..5026)
                     /gene="rsmB"
                     /locus_tag="PK242_07580"
     CDS             complement(3698..5026)
                     /gene="rsmB"
                     /locus_tag="PK242_07580"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOZ93915.1"
                     /translation="MPSAPTQTLPLWRQLQACARLVAGVRAGRSLTAELAGVDALLRP
                     GAQALSFHVLRWLGLAQALRAQLARRPPPPAVDALLCTSLALAQPADGAPYEAFTLLD
                     QSVEAAKRDRTLRPHAAFINACLRRYLRERAALTAAVQHDPVARWNHPRWWVERLQRD
                     HPQHWQAILAAAQQAAPMDLRVNVQRIGVADYGARLAQQGIGADALGGAALRLHRPRT
                     VQALPGFADGQVSVQSATAQRAAPLLLQGCLDVDAPRILDACAAPGGKTAHLLELRPD
                     ARVTALELDPQRGARISDTLARLGLHAEVRVADAADLPSWWRGERYDAILLDAPCSAS
                     GIVSRHPDVRWLRREADLAQLSAQQDRLLQALWPLLRPGGHLLYCTCSVFAQEGRERV
                     QAFLARHSDAIPCPAPGHILPCAGEAAAASGDNAGCDDGFYYALLRKRLD"
     gene            complement(5026..5604)
                     /locus_tag="PK242_07585"
     CDS             complement(5026..5604)
                     /locus_tag="PK242_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOZ93916.1"
                     /translation="MWVWGVWIAAALLLFWGIGAYNRLVRLRAAVARAFAALDEQLVR
                     QLVWIQGGLPEALRDGPHTTPGELNDEVTAAWGRLHAASEQFAVALAQARAQPLDVAA
                     MAALVMSHEALRTAWAHALADAVPPDAVPSADRLQARWMRLLHQSLPLRAAFNDAAQS
                     YNQAIRQFPASLLAHLFGFQPAGTVTRLAEGR"
     gene            complement(5681..9913)
                     /gene="rpoC"
                     /locus_tag="PK242_07590"
     CDS             complement(5681..9913)
                     /gene="rpoC"
                     /locus_tag="PK242_07590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOZ93917.1"
                     /translation="MKSLLDLFKQFTPDEHFDAIRIGLASPEKIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIDLAAPCAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAYVVTDPGMTPLKKFSIM
                     SEDDYDAKRKEYGDEFVARMGAEGIKELLESIDLDTEIERLRGDLTGSEVKVKKNAKR
                     LKVLEAFKKSGIKPEWMVLNVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNSR
                     LRRLLELKAPEIIARNEKRMLQEAVDSLLDNGRRGKAMTGANKRALKSLADMIKGKSG
                     RFRQNLLGKRVDYSGRSVITVGPTLMLHQCGLPKLMALELFKPFIFARLEQMGIATTI
                     KAAKKEVEAGTPVVWDILEEVITEHPVLLNRAPTLHRLGIQAFEPILIEGKAIQLHPL
                     VCAAFNADFDGDQMAVHVPLSVEAQMEARTLMLASNNVLFPASGEPSIVPSQDVVLGL
                     YYTTRERINGKGEGMVFADIGEIERALAAGEVELTAKVSVRLTEWSKDKATGELTPST
                     SLVETTAGRALLSEILPKGLPFSLMNKALKKKEISRLINASFRKCGLKETVVFADKLL
                     QNGFRLATRAGISIAIEDMLVPGEKAGIIERAEREVKEIEQQYVSGLVTAGERYNKVV
                     DIWGKAGDEVSKVMMSQLAKQKTLDRHGKEVEQESFNSIYMMADSGARGSAAQIRQLA
                     GMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRR
                     LVDVTQDLVVTEEDCGTSDGSLMRAIVEGGEVVESLRERILGRTAAEDLVNPETQEVV
                     VQAGQMLDEDAVDELEGAGIDEVRVRTALTCGTRFGICAKCYGRDLGRGGLVNVGEAV
                     GVIAAQSIGEPGTQLTMRTFHIGGAASRAAVASNVEAKTAGNIGFNPTMRYVTNTKGE
                     LVVISRSGEITIHDEHGRERERHKVPYGAILTVKADQAIKAGAILANWDPLTRPIITE
                     YAGTVKFENVEEGLTVAKQVDEVTGLSTLVVIDPKRRGSAKIVRPQVKLVDAGGNEVK
                     IPGTDHSVTIGFQVGALIQVRDGQDVGPGEVLARIPVEGQKTRDITGGLPRVAELFEA
                     RSPKDKGVLAEQTGTISFGKETKGKIRLQITDPDGKVWEELIPKEKNLLVHEGQVVNK
                     GESIVDGPADPQDILRLLGIDELSRYIVDEVQDVYRLQGVKINDKHIEVIVRQMLRRV
                     VVDNPGDSGYIAGEQVERSEMLDTNDALRAEGKLPATYTNLLLGITKASLSTDSFISA
                     ASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPAGSGLAYHQARKAKDQMDEAER
                     RAIAEAEAAELAGATEMTEAGESANLDGAAAE"
     gene            complement(9937..>10712)
                     /gene="rpoB"
                     /locus_tag="PK242_07595"
     CDS             complement(9937..>10712)
                     /gene="rpoB"
                     /locus_tag="PK242_07595"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806984.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOZ93918.1"
                     /translation="PADIVLNPLGVPSRMNVGQVLEVHLGWAGKGIGQRINEMLQAEG
                     RVAKIRKYLSELYNSGDRKEDLSQLSDDQLLEMAGNLTGGMPFATPVFDGATEEEILD
                     MLQLAYPEDVAASKGLTPTRTQAWLYDGRTGDRFERPTTIGYMHFLKLHHLVDDKMHA
                     RSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASYTLQEMLTVKSDDVQGRTK
                     VYESIVKGEHAIDAGMPESFNVLVKEIRSLGLDIELERS"
BASE COUNT         1606 a         3551 c         3759 g         1796 t
ORIGIN      
        1 cacgcggatt ttcaatccgc tgctctacca actgagctac cgggcctgga aaccgaaaat
       61 tctagcacac gcctcagagt gccacggcag cgccacggcg gttgcgcgac aggtcgaccc
      121 ccagctgctt gagcttgcgg tacaggtgcg tgcgctccag cccggttttt tcagccacgc
      181 gggtcatgga gccgccttcc ttggccagat ggaactcgaa gtaggctttt tcgaaggcgt
      241 cgcgcgcgtc gcgcaggggt ttgtcgagat cgaaggtctg ctggccctgc gggctcatgt
      301 ctagggccgc gggcaggctc tggccaccgg tcatggcggc ttccaccgtc aggtccgccg
      361 gggcatgcac gacgacaccg gcctcgacca cctcggccac ggcgcggcgg gccggcacgt
      421 cgcgcgacag cgcatgatcg acggtcttga gcagcttctg cagggtgatg ggcttttcca
      481 gaaacgactg cgcgccgatg cgcgtggcct cgaccgcggt gtcgatggtg gcgtgaccgc
      541 tcatcatgat gaccggcatg gtcagcaggc cggcgccgcg ccattccttg agcagggtga
      601 cgccatcggt gtcgggcatc cagatgtcga gcagcaccag atcggggcgg ccgttctggc
      661 gtgcggcgcg tgcctgtgcc gcgttctcgg ccagttcgac ggtgtgccct tcgtcattga
      721 ggatctcgga caggagatcc cgaatgccga gttcgtcgtc aaccaccagg atgttggcca
      781 taagcagtgc gggtcgtgaa gcggagcggg actaggtcag ggcggtgcgc ccttcggagg
      841 ctggctggaa tgataacgat acttgcgccc ccagaatctg gccatcgttg atgcgattgg
      901 atacatcgat gcgggcgccg tgctcgtcgg cgatcttgcg caccacggcc aggcccaggc
      961 cggtgccctt gggtttggtg gtgacgtagg gttcgaaggc gcgcttgagg atggcctcgg
     1021 caaagccgtg gccgctgtcg atcaccgcca gccgcacgcg ctgcaggttg tcctgccact
     1081 gggtgcgcac gatcacgcct tgcgcctggg cttgcgggcc gtcacgcacc acgccttcgc
     1141 tggcgtcgat ggcgttctgc accaggttgt ggatgacctg gcgcaactgc tgggcgtcgc
     1201 ccaggatggg cgggcagtgg gggtcgctct cgaagtgcac gggcacggcc tggcggtgct
     1261 gcacgtcgcc ggcgccgcca tacagctgca gcacttcggt gaccagggcg ttgaggtcga
     1321 ccggcttgag gtcggccatg ggcaggcggg cgtagtcgcg gaattcgttg accaggcgct
     1381 tcatggcgtc gacctgatcg acgatggtgc gcacggcgcg caccagcagg gcgcgctcgg
     1441 cttcgggcag tttgtcggcc agcttgtgct ccagccgctc ggccgacagc tggatggggg
     1501 tgagcgggtt cttgatttcg tgcgccagcc gccgcgccac ttcgccccag gcgtgggcac
     1561 gctgggcgga gacgatttcg gagatgtcgt cgaacaccag cagccgcagg ccgtccggca
     1621 gcggagcgcc gcgggcaatc agggtcaggg cgtcggtggc cacgccgccg cccgggtggc
     1681 cgtacagctc gaacgacttc tgccagtttt ccaggccatg ttcgccgcgc tcgccttcaa
     1741 agctggcgaa ctgggtctgg acttcggcgg caaaggcctg caggccctcc aggctgccca
     1801 gcgggcggcc ggcgctggcg gccagcggca cgcgcaggat gcgcgtggcg cccgggttgg
     1861 acgactggat caggccctgg gcatcgagca cgatgacgcc ggcggtcagg ttgtcgagga
     1921 tggtctgcag gcgcgcccgc gaggcgtcca ccgcgtgcat gctggcgtcg agggcggcgc
     1981 gggcgtcggc cagctgctgg gtcatgtcgg cgaacgagcg ggtcaggccc accagttcgt
     2041 cgcgcccggg caggatcacc ttcggcgtca ggtcgccggt ggccacctgg cgcatgccat
     2101 cggccagcat cagcagcggc gtggccagct gcttgcccag tgccacggcc agcagcacgg
     2161 cgccgaacac ggccagaaac aggctcaggg tgagggtgcc gatgtacatg cgccgcaggc
     2221 cttcgcggcc cagggcgcgt tcctggtatt cgcgatgcgc ttcctgcacc gccagggcgt
     2281 gcgtgaccag gctggtgggc agcggctgca ccgcctgcag caggcgctgc tcgcccacca
     2341 ggccgatggt gcgggcactg accgccacca gggcgcggac gcgggcttcg ggggtggtgc
     2401 cgccgggcac gacgtcgtcc agtccctcga tctgcgtggc cacgccctgg gcccgcaggt
     2461 tgcgcagcat ggcgccactg gggcgctccg gcgtgagctg aaagcgcgac cgtccggcgc
     2521 tggccagcag acggcccgac gcgctccaca gggtcaggtc gctggcgccc agttgcacgc
     2581 gcagttgctc cagcggcaac gccgccagcg catcgggcac gtccgccagc gcgaccgaag
     2641 cggtgcgcaa ctggttgccg aactcgcgcg cctgggtatc cagcgaggtg cgcgccagcg
     2701 tgaggccgga atccagcgcg ccttcgaccc gcacgtcgaa ccacgattcg atcgagcgcg
     2761 acacgaactg ataggacacg acgtagatca gcaaccccgg caccaccccc accaacccga
     2821 agatggcggc cagcttgatc agcagccggc tgccgaactt gccgcgccgc aggcgcatga
     2881 gcaagcggta caccccccac agaatgacca gccccagcac ggcggcgacg accacgttga
     2941 cgatgaacag ccgtgtgtag tagcgctcgt acagcaagcg gttgttggtg gcctgggcca
     3001 gcaggaacag cagcaccagg ccgatgccca ccagcagggt gccggcggcc cacagcgccc
     3061 agcgcgaacg gcggcggcgc gcgctctggg ccgggtcggc ggcggcggcg gcggtactca
     3121 ctgggccagc tcctgcgtca ggtcgatgcg gcgctcgacc gagatcaccc agtcggaatg
     3181 cccgacggag ccgatttgca gggtacgcgg cagctggctg gcgtcgagcc ggaagcgaaa
     3241 gcgcagcaga tggcggccgc cgtcgtcgat gtcggcggcg ctggcgatgc gccagcggcc
     3301 aatgcgctgc acggcggtga gggcttcgtc cagggtgtcg aagctctgcg tcaggctgac
     3361 gcccaggccg gcattggtca gcggttcgga cgaagtgttc aggcgccagc ggcgcgtcag
     3421 gggctggtag gacaggcgca ggtagcgccg cgccagggcc acgctgcggt cggcccagta
     3481 ccagcgctcg cgcagcactt cggcctcggc cacgaagtgc accggaatgc ctttgtgcag
     3541 ggcgtcttcg accaccggtg acagggcaaa gtcgaggcgg gccgacagga tcagctcgtt
     3601 accctcgcgc gcgacgctga gcgcgctcag ggcggcgccc tcggcacggg cgacggcgcc
     3661 caggccgcag gccagcgccc aggccagcag caggcggcta atccagacgc ttgcgcagca
     3721 gcgcgtaata aaacccgtca tcacagcccg cattgtcacc actggcggcg gccgcctcgc
     3781 cggcgcaggg caatatatgg ccgggcgcgg ggcatggtat ggcgtcactg tggcgtgcaa
     3841 gaaacgcctg cacgcgctca cgcccttcct gggcgaacac cgagcaggtg cagtaaagca
     3901 ggtggccgcc tggccgcagc agcggccaca gcgcctgcag cagacggtcc tgctgggcgg
     3961 acagctgtgc aaggtccgct tcgcgccgca gccagcgcac gtcggggtga cggctgacga
     4021 tgccggaagc gctgcagggg gcgtcgagca agatggcgtc gtagcgctcg ccccgccacc
     4081 aggacggcag gtcggcggcg tcggccacgc gcacctcggc gtgcaggccc aggcgcgcca
     4141 gcgtatcgct gatgcgggcg ccgcgctgcg gatcgagctc cagcgcggtg acgcgggcgt
     4201 ccgggcgcag ctccagcagg tgggcggtct tgccgccggg agccgcacag gcgtccagga
     4261 tgcgcggcgc gtcgacatcg aggcagccct gcaacagcag gggcgcggcg cgctgggcgg
     4321 tggccgattg caccgagacc tggccgtcgg cgaaacccgg cagcgcctgc accgtgcgcg
     4381 gccggtgcag gcgcagggcg gcgccgccca gcgcgtcggc accgatgccc tgctgggcca
     4441 ggcgcgcgcc gtagtcggcc acgccgatgc gctggacgtt gacgcgcagg tccatcggcg
     4501 ccgcctgttg tgccgccgcc aggatggcct gccagtgctg cgggtgatcg cgctgcagcc
     4561 gctcgaccca ccagcgcggg tggttccagc gcgccaccgg gtcgtgctgc acggccgcgg
     4621 taagggccgc gcgctcgcgc aggtagcgcc gcaggcaggc gttgatgaag gcggcgtgcg
     4681 ggcgcagggt gcgatcgcgc ttggcggctt cgacggactg atcgagcagg gtgaaggctt
     4741 cgtagggcgc gccgtcggcc ggctgcgcca gcgccaggct ggtgcacagc agggcatcga
     4801 cggcgggcgg tggggggcgg cgcgccagct gggcgcgcag cgcctgcgcc agcccgagcc
     4861 agcgcagcac atgaaacgac agcgcctggg cgccggggcg cagcagggca tcgacgcccg
     4921 ccagttcggc cgtcagcgaa cgccccgccc gcacgcccgc caccagccgc gcacaggcct
     4981 gcaactggcg ccacaggggc agggtttgcg tgggggcgga aggcatcagc gaccttcggc
     5041 cagtcgggtg acggtgccgg ccggctgaaa accgaacagg tgcgccagca gcgatgccgg
     5101 aaactggcga atcgcctggt tgtagctctg cgcggcgtcg ttgaaggcag cgcgcaaggg
     5161 cagcgactgg tgcagcaggc gcatccagcg cgcctgcagg cggtcggccg agggcacggc
     5221 gtcgggcggc acggcgtcgg ccagcgcgtg cgcccaggcg gtgcgcaagg cttcatgcga
     5281 catcaccagg gccgccatgg ccgccacgtc cagcggctgc gcgcgcgcct gcgccagcgc
     5341 cacggcgaat tgctcgctgg ccgcgtgcag gcggccccag gcggccgtga cctcgtcgtt
     5401 cagttcaccc ggcgtggtgt gcggaccatc gcgcagggct tccggcaggc caccctggat
     5461 ccagaccagt tgccgcacca gctgctcgtc cagcgccgca aacgccctgg ccaccgcggc
     5521 acgcaggcgc accaggcggt tgtaggcacc gatgccccag aacagcagca gcgccgccgc
     5581 gatccacacg ccccacaccc acatcccgca ctccccaaga aaaagcgccc tcagcgcttt
     5641 agcagcatgc ggagggcgct atcaaacaaa gagcaatcgc ttactcggcg gcagctccgt
     5701 ccaggttggc ggactcgccc gcttcggtca tttcggtggc gccggccagc tcggccgcct
     5761 cggcttcggc gatggcgcgg cgctcggcct cgtccatctg gtccttggcc ttgcgcgcct
     5821 ggtggtaggc caggccggaa cccgccggga tcaggcggcc gacgatgacg ttttccttca
     5881 gaccgcgcag ctcgtcgcgc ttgcccatga tggccgcttc ggtgagcacg cgcgtggttt
     5941 cctggaagga agccgccgag atgaacgaat cggtggagag cgaggccttg gtgatgccca
     6001 gcagcaggtt ggtgtaggtg gccggcagct tgccttcggc gcgcagggca tcgttggtgt
     6061 ccagcatttc gctgcgctcg acctgctcgc cggcgatgta gccggagtcg cccgggttgt
     6121 cgaccaccac gcggcgcagc atctggcgca cgatgacctc gatgtgcttg tcgttgatct
     6181 tcacaccttg caagcggtag acgtcctgca cctcgtcgac gatatagcgc gacagctcgt
     6241 cgatgcccag caggcgcagg atgtcctgcg ggtcggccgg accgtcgacg atggactcgc
     6301 ccttgttgac cacctggcct tcgtgcacca gcaggttctt ttccttggga atcagctctt
     6361 cccagacctt gccgtcgggg tcggtgattt gcaggcggat cttgcccttg gtttccttgc
     6421 cgaacgagat ggtgccggtc tgttcggcca gcacgccctt gtccttgggc gaacgggctt
     6481 cgaacagctc ggccacgcgc ggcagaccgc cggtgatgtc gcgcgtcttc tggccttcga
     6541 ccggaatgcg cgccagcacc tcgccggggc cgacgtcctg gccgtcgcgc acctggatca
     6601 gcgcgccgac ctggaagccg atggtcaccg agtggtcggt accggggatc ttgacctcgt
     6661 taccgccggc gtcgaccagc ttgacctgcg gccgcacgat cttggccgaa ccgcgacgct
     6721 tgggatcgat caccaccagg gtggacaggc cggtcacttc atcgacctgc ttggccaccg
     6781 tcaggccttc ctcgacgttc tcgaacttca ccgtaccggc gtattcggtg atgatggggc
     6841 gtgtcagcgg gtcccagttg gccaggatgg cgccggcctt gatggcctgg tcggccttga
     6901 cggtcaggat ggcgccgtag ggcaccttgt ggcgctcgcg ttcgcggccg tgctcgtcgt
     6961 ggatggtgat ttcgcccgaa cgcgagatca ccaccagctc acccttggtg ttggtgacgt
     7021 agcgcatggt cgggttgaag ccgatgttgc cggcggtctt ggcttcgacg ttggaagcca
     7081 cggccgcgcg cgacgccgca ccaccgatgt ggaaggtgcg catggtcagc tgcgtgccgg
     7141 gctcgccgat cgactgcgcg gcgatcacgc ccaccgcctc gccgacgttg accaggccgc
     7201 cgcggcccag gtcgcggccg tagcacttgg cgcagatgcc gaaacgggtg ccgcaggtca
     7261 gggcggtgcg cacgcgcacc tcgtcgatgc cggcgccttc cagttcgtcg accgcgtctt
     7321 cgtcgagcat ctggcccgcc tgcaccacca cttcctgagt ctctggattg accaggtcct
     7381 ctgccgcggt gcggcccagg atgcgctcgc gcagcgattc gaccacctca ccgccttcga
     7441 cgatggcgcg catcagcgag ccgtcgctgg tgccgcagtc ttcctcggtg acgaccaggt
     7501 cctgcgtcac gtcgaccaga cggcgcgtca gataaccgga gttggcggtc ttcagcgccg
     7561 tgtcggccag acccttgcgc gcgccgtggg tggagatgaa gtactgcagc acgttcaacc
     7621 cctcgcggaa gttggccgtg atgggggttt cgatgatcga gccgtccggc ttggccatca
     7681 ggccgcgcat gccggcaagc tgacggatct gggccgcgct gccgcgggca cccgagtcgg
     7741 ccatcatgta gatggagttg aaggactcct gctcgacttc cttgccatgg cggtccagcg
     7801 tcttctgctt ggccagctgg ctcatcatga ccttggacac ttcgtcgccg gccttgcccc
     7861 agatgtcgac caccttgttg tagcgctcgc cggcggtgac caggcccgag acgtactgct
     7921 gctcgatttc cttgacctcg cgctcggcgc gctcgatgat gccggccttt tcgcccggca
     7981 ccagcatgtc ctcgatggcg atcgagatgc cggcgcgcgt ggccaggcgg aagccattct
     8041 gcagcagctt gtcggcaaac accacggttt ccttcaggcc gcacttgcgg aagctggcgt
     8101 tgatgaggcg cgagatttcc ttcttcttga gcgccttgtt catgagcgag aacggcaggc
     8161 ccttgggcag gatctcggac agcagggcgc gaccggccgt ggtttcgacc aggctggtgg
     8221 atggcgtcag ctcgcccgtg gccttgtcct tgctccactc ggtcaggcgc acgctcacct
     8281 tggcggtgag ctcgacctcg ccggcggcca gcgcgcgctc gatctcgccg atgtcggcga
     8341 agaccatgcc ctcgcccttg ccgttgatgc gctcgcgcgt ggtgtagtac aggcccagca
     8401 ccacgtcctg cgagggcacg atggagggtt caccggaggc cgggaacagc acgttgttgg
     8461 aagccagcat cagggtgcga gcttccatct gcgcctccac cgacagcggc acgtgcacgg
     8521 ccatctggtc accgtcgaag tcggcgttga aggcggcgca caccagcggg tgcagctgaa
     8581 tggccttgcc ttcgatcagg atcggctcga aggcctggat gcccaggcgg tgcagcgtgg
     8641 gcgcgcggtt gagcagcacc ggatgctcgg tgatgacctc ttccaggatg tcccacacga
     8701 ccggcgtgcc ggcctcgacc tctttcttgg cggccttgat ggtggtggcg atgcccatct
     8761 gctccagacg ggcgaagatg aagggcttga acagctccag cgccatcagc ttgggcaggc
     8821 cgcactggtg cagcatgagc gtcgggccca cggtgatgac cgagcggccc gagtagtcga
     8881 cgcgcttgcc cagcaggttc tggcggaagc ggccgctctt gcccttgatc atgtcggcca
     8941 gcgacttgag ggcgcgcttg ttggcgccgg tcatggcctt gccacggcgg ccgttgtcca
     9001 gcaggctgtc gacggcttcc tgcagcatgc gcttttcgtt gcgcgcgatg atctcgggcg
     9061 ccttgagttc cagcaggcgg cgcaggcgcg agttgcggtt gatgacgcgg cggtacaggt
     9121 cgttcaggtc cgaggtggcg aaacgcccgc cgtccagcgg caccagcgga cgcagatcgg
     9181 gcggcagcac cggcagcacg ttcagcacca tccactcggg tttgatgccg gatttcttga
     9241 aagcctccag cactttcagg cgcttggcgt tcttcttgac cttgacctcg gagcccgtca
     9301 ggtcaccgcg caggcgctcg atttcggtat ccaggtcgat gctctcgagc agttccttga
     9361 tgccttcggc gcccatgcgc gcgacgaatt cgtcgccgta ttccttgcgc ttggcgtcgt
     9421 agtcgtcctc gctcatgatg ctgaacttct tcagcggcgt catgccgggg tcggtgacca
     9481 cgtaggcttc gaagtacagc acgcgctcga tgtcgcgcag cgtcatgtcg agcaccatgc
     9541 ccagacggct gggcagcgac ttcaggaacc agatgtgggc gcagggcgcg gccaggtcga
     9601 tgtggcccat gcgctcgcgg cgcaccttgg tctgcgtgac ttcgacgccg cacttctcgc
     9661 agatcacgcc gcggtgcttc aggcgcttgt acttgccgca caggcactcg tagtccttga
     9721 tcgggccgaa gatcttggcg cagaacaggc cgtcgcgctc gggcttgaag gtgcggtagt
     9781 tgatggtttc gggctttttc acctcgccga aggaccagga acggatcttc tcgggcgagg
     9841 ccaggccgat gcggatggca tcgaagtgct catccggcgt gaattgcttg aacaggtcga
     9901 gcagcgattt catgtgactc tttccttttc aatgaatcag gagcgctcca gctcgatgtc
     9961 gagaccgagc gagcggattt ccttgaccag cacgttgaac gactcgggca tgccggcgtc
    10021 gatcgcgtgt tcgcccttga cgatggattc gtacaccttg gtgcggccct gcacgtcgtc
    10081 ggacttgacg gtgagcattt cctgcagcgt gtaggaagcg ccgtaggctt ccagcgccca
    10141 cacttccatc tcgccgaagc gctggccgcc gaactgcgcc ttgccgccca gcggctgctg
    10201 cgtcaccagc gagtacggac cggtggaacg cgcgtgcatc ttgtcgtcca ccaggtggtg
    10261 cagcttcagg aagtgcatgt agccgatggt ggtgggccgc tcgaaacgat caccggtgcg
    10321 gccgtcgtac agccaggcct gggtgcgggt gggcgtcaga cccttgctgg ccgccacgtc
    10381 ctcgggatag gcgagctgca gcatgtcgag gatttcttcc tcggtggcac cgtcgaacac
    10441 cggcgtggca aagggcatgc cgccggtcag gttgcccgcc atttcgagca gctgatcgtc
    10501 gctgagctgg ctcaggtctt ccttgcggtc gccgctgttg tagagctcgc tcaggtactt
    10561 gcggatcttg gccacgcggc cctcggcctg cagcatctcg ttgatgcgct gaccgatgcc
    10621 cttgccggcc cagcccaggt gcacttccag cacctgaccc acgttcatgc gcgaaggcac
    10681 gcccagcggg ttgagcacga tgtcggcggg cg
//
LOCUS       DAOOOG010000083        10624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_7072_length_10624_cov_25.135869, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000083 DAOOOG010000000
VERSION     DAOOOG010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10624
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7072_length_10624_cov_25.135869"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..520)
                     /locus_tag="PK242_07600"
     CDS             complement(50..520)
                     /locus_tag="PK242_07600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ93919.1"
                     /translation="MAESISPRLAAIVAALPLRPGMRVLEIGCGPGVAARAVVRRMGQ
                     GHVLAIDRSAKAIAQAMAGSAAELASGRLALRHVALEDLRLGVDEPAYDLAFAVRVGA
                     LDGRHPAAGRIALERLRAALTPEGRLFIDGGDPLLEIALGPSRPLAPTARGGPA"
     gene            complement(513..1136)
                     /locus_tag="PK242_07605"
     CDS             complement(513..1136)
                     /locus_tag="PK242_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOZ93920.1"
                     /translation="MTQAQALSTAHCSVFIATSLDGFIARPDGAIDWLTGADAADNAG
                     EDYGYAAFLSSVDAIVMGRASLEKVLTFDAWPYGQMPVYALSSSLRALPAKAPGSVSL
                     LDAEPAAVAALAARNGHGRLYVDGGVTIQRFLRAGLIRDMVITTVPVLLGAGRRLFGP
                     LAADVALDLTDSRAYANGLVQSRYKVRHGAGEAAAAGGAPGRGPGHG"
     gene            complement(1161..1451)
                     /locus_tag="PK242_07610"
     CDS             complement(1161..1451)
                     /locus_tag="PK242_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HOZ93921.1"
                     /translation="MNVPHTSHTPEQIDRLARRRAGAKMGWFIHAFVYLCVNAGLALL
                     ALSHGRDWYAYPLLGWGLGLALHGAAVWLMGPGAHWRERLVERERALLARRN"
     gene            1688..2512
                     /locus_tag="PK242_07615"
     CDS             1688..2512
                     /locus_tag="PK242_07615"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOZ93922.1"
                     /translation="MDVLLMVKAALMGVVEGLTEFLPISSTGHLILAGSLLGLTGDKV
                     KVFEIAIQTGAILAVVIVYWHKIRATLVELPRSRQAQRFALNVVIAFVPAVLLGLLFG
                     KAIKAHLFTPQVVASTFILGAFVILWAERRPQSAARVHRVDDMTALDALKVGLVQCFA
                     MIPGTSRSGATIIGGMLLGLSRQAATDFSFFLAIPTLIGAGAYSLWKERALLSWADLP
                     LFGIGLLFSFLSAWICVRWLLRYIASHSFVPFAWYRLAFGAVVLLTWWMGWVAWVD"
     gene            2648..3625
                     /locus_tag="PK242_07620"
     CDS             2648..3625
                     /locus_tag="PK242_07620"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HOZ93923.1"
                     /translation="MEIFDYDHILLLPRKCRVQSRSECDAGIDFGGRRFRLPVVPANM
                     KTVVDEKLCIWLAEHGYFYVMHRFDFDNLAFVRDMKARGLYASISVGIKPADYACIDQ
                     LAAEGLTPEYITIDIAHGHSDQVRDMIAHIKARLPASFVIAGNVATPEAVIDLENWGA
                     DATKVGVGPGKVCITRLKTGFGTGGWQLSAVKWCARVATKPIVADGGIRHHGDIAKSI
                     RFGATMIMIGSLFAGHEESPGRTVEVDGVQYKEYYGSASDFNKGEYKHVEGKRILEPI
                     KGKLADTLIEMEQDVQSSISYAGGRKLMDVRKVNYVILGGDNAGEHLLM"
     gene            complement(3862..4761)
                     /locus_tag="PK242_07625"
     CDS             complement(3862..4761)
                     /locus_tag="PK242_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ93924.1"
                     /translation="MNLRQLDHLVALADTGSFTRAAARLHLTQSALSRSVQALEDELG
                     APLVDRIGKRSELTPLGQAVLARARRIGLEAAEIRRSAEVLRQGEAGTMRVGLGSGPG
                     GLLMTPLLTHVAGLHPKVRVQIARGSTELQLQQLRARELDALVLDARRVTPAPDLVIE
                     PAVELRAGFVCRAGHPLAASTAVTFEMLMRYPVATTPLSDEVARVLVARYGPGAEPVR
                     MASLVCEDVGSLLDAVAQTDAVFLGAVAAARAAIDADRLVELRMTPPLDSCARYAYVR
                     LAGRTEAPVMQVLRRFAAERLRD"
     gene            4882..5880
                     /locus_tag="PK242_07630"
     CDS             4882..5880
                     /locus_tag="PK242_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ93925.1"
                     /translation="MSSARRPLLAAIVCAVALGGFAGTAGANSFPSKPVSIMVPYPAG
                     GISDTIARRVNVPLGQALGQTVIVENLGGASGSIAAQKVLNAPADGYNMFQGSPNELI
                     LAPLAIPGIKYKPEDFRQVHRISLGPMAIFARANLPAGNADELSAYAAKVAKEGKPMT
                     YASVGVGSFYHLLGGKMSKDIGVEMTHVPYKGAADVARDLAGGQVDIFITPYGPSLVE
                     LVKQGKLKVVAALSTQRQPAIPDVPTTAESKALKNFQYQIGTGYFVKRSTPEPVVQTL
                     HKGLQKVLSDAEVKAALEANGAQVSPPQTLAEADAAFKQETATYQAIARSIGLVQP"
     gene            5877..7100
                     /locus_tag="PK242_07635"
     CDS             5877..7100
                     /locus_tag="PK242_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HOZ93926.1"
                     /translation="MSSAVAQAPHALLTHLQGGAEAFVALRRDIHRHPELGYQEVRTS
                     DLVAERLTAWGYQVTRGLGGTGLVGQLRRGSGQKRLGLRADMDALPIQEATGLPWASQ
                     HAGRMHACGHDGHTAMLLAAAHHIAEHGRFSGTLNLIFQPAEEGLGGAKAMMDDGLFE
                     RFPCDAIFAMHNMPGYPQGKLLLREGPLMASSDGLTITLTGKGSHAAVPQHGIDPVVA
                     GSSIVLGLQSIVARNVDPQAMAVITVGAFQAGHAHNVIPGEAVLKLSVRALDRKVRAT
                     LLRRIEELVDAQARAFGVGWRMERVTGYPVLVNTRAETEFARRVATELVGAARVEPQT
                     VALSGSEDFAFMLEQVPGSYLLIGNGDGSDDGANGGHGACMVHNPGYDFNDANLPVGA
                     AYWALLVERFLSDAA"
     gene            complement(7171..8046)
                     /locus_tag="PK242_07640"
     CDS             complement(7171..8046)
                     /locus_tag="PK242_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ93927.1"
                     /translation="MARPNDSSRRDQPDTQWLERMYNNLRRVPDHGDYFARWAAESAL
                     VRRSLPCQLDLPYGDAAGETLDAFPAERPRSPIVVFIHGGYWKAMDKSQHSFVVPALR
                     ALGAAVVVPNYTLAPKASIPDITLQMVRAVAWCGRHASALRGDARRLVVIGHSAGGQL
                     AAMLLGCAWSTFDPALPPRLVRAALGISGLYDLQPLLHTPSLQEVLRLTPRQVQVASP
                     ARLPAPAHGRLISVVGGEESGEYLRLNKLIQQAWGRERVPVAAELPGLNHFSILDTLA
                     APRSRLHRLAAGLLV"
     gene            8179..9048
                     /locus_tag="PK242_07645"
     CDS             8179..9048
                     /locus_tag="PK242_07645"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ93928.1"
                     /translation="MTLTLLGTGRMGLPMARRLCQAGHELHVWNRSIDKAQPLAADGA
                     TVHGDLASAAAAGDIVISLLENGPVVASVLFEQGAAAALRPGSLFIDMASIKPAEARE
                     HAARLAERGVRHLDAPVSGGTVGAEAGTLAIMVGGEVQDFERARPVFDVLGRATHVGP
                     HGAGQLAKLANQMIVGITIGAVAEALLLCQKGGADPAKVRQAIGGGFAQSRILELHGE
                     RMVTRDFAPRARMTVQLKDLRNALDTAQAMGFEAPVTAALERLYADGIAHGDSELDHA
                     GLFVELARRNGMS"
     gene            9127..10473
                     /locus_tag="PK242_07650"
     CDS             9127..10473
                     /locus_tag="PK242_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOZ93929.1"
                     /translation="MSLYAQLQQRAAAGRPIRVGLIGAGKFGSMYLAQVPRTPGVHLA
                     AIADLSPAAARANLARVGWRPEQAQAASIDEALQRGSTHVGEDWQALVRHPAIDVVVE
                     CTGHPIAAVDHCLAAFDAGKHVVNVTVEADAFCGPLLARRAAESGVVYSLAFGDQPAL
                     ICDLVDWARTCGFPVVAAGRGHKWLPHFCESTPETVWGYYGLSPEQAARGGLNPKMFN
                     SFLDGSKPAIESTAVANATGLAVPTGGLQYPSGSIADIPRLTRPRSAGGVLEKKGMVE
                     VISSLRPDGTPIDYDIRMGVWVTVEAETDYIRHCFEEYNAHTDDSGRYFTLYKRWHLI
                     GLEVGLSVASVALRREATGVAQGWHADVVATAKRDLKPGDVLDGEGGTTVWGKLQPAS
                     RSVAMGGLPLGLAHDVKVLRPVTKGQCLSWDDVAMDTRTRAWQLRQQMQQLLTPAD"
     gene            10500..10575
                     /locus_tag="PK242_07655"
     tRNA            10500..10575
                     /locus_tag="PK242_07655"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10533..10535,aa:Ala,seq:cgc)
BASE COUNT         1671 a         3697 c         3692 g         1564 t
ORIGIN      
        1 aacgccacga ccagcagaaa atgccggccc acgtcgcgcc aggcgatgct catgccggcc
       61 ctccgcgcgc ggtaggcgcg agcgggcggc tcgggccgag cgcgatttcc agcagcggat
      121 cgccgccatc gatgaacagc cgcccttccg gggtgagcgc cgcacgcagc cgctcgaggg
      181 cgatgcggcc cgcggccgga tggcggccgt cgagcgcacc gacgcgcacg gcaaaggcca
      241 ggtcgtaggc gggctcgtcc acgccgagcc gcaagtcctc gagcgcgaca tggcgcagcg
      301 cgagcctgcc cgacgcgagc tcggccgccg agcccgccat ggcctgggcg atggccttgg
      361 ccgagcggtc gatggcaagc acatggccct gccccatgcg ccgcaccacc gcacgcgcgg
      421 cgacgcccgg gccgcagcca atctccagca cgcgcatccc cggccgcagc ggcagcgccg
      481 cgacgatggc ggccagacgc ggggatatgg actcagccat ggcccgggcc cctgccgggc
      541 gcaccacccg ccgcggccgc ctccccggcg ccatggcgca ccttgtaccg cgactgcacc
      601 aggccgttgg cataggcgcg cgagtcggtc agatcgagcg ccacgtcggc cgccagcggg
      661 ccgaacaggc gccggcccgc gcccagcagc accggcacgg tggtgatgac catgtcgcgg
      721 atcaagcctg cgcgcagaaa gcgctggatg gtgacgccgc cgtcgacgta caggcgccca
      781 tgcccgttgc gcgcggccag ggcggcgacg gcggccggct cggcgtcgag cagcgacacg
      841 ctgcccggcg ccttggccgg cagcgcccgc aggctgctgc tgagcgcgta gaccggcatc
      901 tgcccatagg gccaggcgtc gaaggtcagc accttctcca gcgaggcccg gcccatgacg
      961 atggcatcga ccgaggacag aaacgcggca tagccgtagt cctcgcccgc gttgtcggcg
     1021 gcgtcggccc ccgtcagcca gtcgatggcc ccgtcgggcc gggcgatgaa gccgtccagg
     1081 ctggtggcaa tgaagaccga gcaatgcgcg gtggacaggg cttgggcttg tgtcatgtgc
     1141 ggcccttttc agtagctcct tcagttgcgc cgcgcgagca gcgcgcgctc gcgctcgacc
     1201 aggcgttcgc gccaatgcgc acccggcccc atcagccaca cggccgcgcc atgcagcgcc
     1261 agccccaggc cccagccgag cagcggatag gcgtaccagt cgcgcccatg gctcagcgcc
     1321 agcagggcca ggccggcgtt gacgcacagg tagacaaagg cgtggatgaa ccagcccatc
     1381 ttggcaccgg cgcgcctgcg cgccagccgg tcgatctgct cgggggtgtg ggaggtgtgg
     1441 gggacgttca tggtgctgct ccttgctgcg atggcgccac tgtgccgccg cgtgccacgg
     1501 cgcgccagca tgatgcgacg aaatgccgcc ggccggcggc aggcgcgcag cgcgccgacg
     1561 gaatgcggcg ctcgcccgca cgccgccggc cattcgcgtt tccgaaaaaa tcagcttcca
     1621 gcgcttgtag aatgtgcgct gacagctaca aatggtgtag caaagttgac ccgaaaagga
     1681 gtggtgagtg gatgtgctgt tgatggtcaa ggcggcgctc atgggcgtgg tcgaaggtct
     1741 gaccgagttt ctgcccatct ccagcaccgg ccacctgatt ctggccggca gcctgctggg
     1801 cttgaccggc gacaaggtca aggtgttcga gatcgccatc cagaccggcg ccattctggc
     1861 ggtggtgatc gtctattggc acaagattcg cgccacgctg gtcgagctgc cccgcagccg
     1921 gcaggcgcag cgctttgccc tcaacgtcgt tatcgccttc gtaccggcgg tgctgctggg
     1981 cctgctgttc ggcaaggcga tcaaggcgca cctgttcacg ccgcaggtgg tggccagcac
     2041 cttcatcctg ggcgccttcg tcatcctgtg ggccgagcgc cggccgcaaa gcgcggcgcg
     2101 cgtgcaccgg gtggacgaca tgacggcgct ggatgcgctc aaggtcggcc tggtgcagtg
     2161 ctttgccatg atcccgggca ccagccgctc gggcgccacc atcatcggcg gcatgctgct
     2221 ggggctgtcg cgccaggcgg cgaccgattt ttcgttcttc ctggcgattc cgacgctgat
     2281 cggcgccggt gcctattcgc tctggaagga gcgcgccctg ttgtcctggg ccgacctgcc
     2341 gctgttcggc atcggtctgc tgttttcgtt tctcagcgcc tggatctgcg tgcgctggct
     2401 gctgcgctac atcgccagcc acagtttcgt gccctttgcc tggtatcgcc tggcgttcgg
     2461 cgccgtggtg ctgctgacct ggtggatggg ctgggtggcg tgggtggatt gagcgcggcc
     2521 tgcagcactg cactagaatc aagcaccggg cccaagtttt cttggcccgg ttttttgtgc
     2581 ctgccacaaa agcccggtgc caggccaagc gccctcgacg tcacactgca caaggagctt
     2641 ggacaccatg gaaatcttcg actacgacca catcctgctg ctgccgcgca agtgccgcgt
     2701 gcaaagccgc agcgaatgcg atgccggcat cgacttcggc ggacgccgct tccggctgcc
     2761 ggtggtgccg gccaacatga agacggtggt cgatgaaaag ctgtgcatct ggctggccga
     2821 gcatggctat ttctacgtca tgcaccgctt cgacttcgac aacctggcct tcgtgcgcga
     2881 catgaaggcg cgcgggctgt acgcgtcgat ctcggtcggc atcaagccgg ccgactacgc
     2941 ctgcatcgac cagcttgccg ccgaggggct gacgcccgag tacatcacca tcgacatcgc
     3001 gcacggccat tcggaccagg tgcgcgacat gatcgcccac atcaaggcgc gcctgccggc
     3061 cagcttcgtg atcgccggca acgtggccac gcccgaggcg gtgatcgacc tcgagaactg
     3121 gggcgccgac gccaccaagg tcggcgtggg gccgggcaag gtgtgcatca cccggctgaa
     3181 gaccggcttt ggcaccggtg gctggcagct cagcgccgtc aagtggtgcg cgcgcgtggc
     3241 caccaagccg atcgtcgccg acggcggcat ccgccaccac ggcgacatcg ccaaatcgat
     3301 ccgctttggc gccaccatga tcatgatcgg ctcgctgttt gccggccacg aggagtcgcc
     3361 cggccgcacg gtcgaagtcg acggcgtgca gtacaaggag tactacggct cggccagcga
     3421 cttcaacaag ggcgagtaca agcacgtgga gggcaagcgc atcctggagc ccatcaaggg
     3481 caagctggcc gacaccctga tcgagatgga gcaggacgtg caaagctcga tcagctacgc
     3541 cggcggccgc aagctgatgg acgtgcgcaa ggtcaactac gtgatcctgg gcggcgacaa
     3601 cgccggcgag cacctgctga tgtgaggccg ccgcgccggg cttgaagtgt tttaggcctc
     3661 tgacgcagtc ccggcaagcg ccggcagcta ttgagttgat agcttgtgtg accccgttcg
     3721 ggctcagcct gtcgaagccc ttcgacaagc tctgggcgaa cggtgaacac ctgatggggc
     3781 cggacaacag gatggcggat gacctcgctg cgcctcgatg acccacggtg catgggtcat
     3841 gttcttgatc ggcaagccgc atcagtcacg caggcgctcg gccgcaaagc gccgcagcac
     3901 ctgcatcacc ggcgcttcgg tgcggccggc caggcgcacg taggcgtagc gcgcgcagga
     3961 atcgagcggc ggcgtcatgc gcagctcgac cagccggtcg gcgtcgatcg ccgcgcgcgc
     4021 ggcggcgacg gcgcccagaa acaccgcatc ggtctgtgcc acggcgtcca gcaggctgcc
     4081 gacgtcctcg cacaccagcg aggccatgcg caccggctcc gccccgggcc cgtagcgcgc
     4141 caccagcacg cgcgccactt cgtccgacag tggggtggtg gccaccgggt agcgcatcag
     4201 catctcgaag gtcaccgccg tgctggcggc cagcggatgg ccggcgcggc agacgaagcc
     4261 ggcgcgcagc tcgaccgccg gctcgatcac caggtcgggc gcgggtgtca cccggcgcgc
     4321 gtcgagcacc agcgcgtcca gctcgcgtgc gcgcaactgc tgcagctgca gctcggtgga
     4381 gccgcgcgcg atctgtaccc gcaccttggg gtgcaatccc gccacatggg tgagcagggg
     4441 cgtcatcagc agcccgcccg gccccgagcc cagccccacc cgcatggtgc cggcctcgcc
     4501 ctggcgcagc acctcggcgc tgcggcggat ttcggccgcc tccagcccga tgcggcgtgc
     4561 gcgtgccagc accgcctggc ccagcggggt gagctcgctg cgcttgccga tgcggtcgac
     4621 cagcggcgcg cccagttcgt cctccagcgc ctgcacgctg cggctgagcg ccgactgcgt
     4681 caggtgcagg cgcgcggcgg cgcgggtgaa cgaaccggta tcggccagcg ccaccaggtg
     4741 atcgagctga cgcaggttca tatcatgagt ttagggaatc atttgtttga acacaattcg
     4801 tttgacgcat tcttgtgatt gcgatttact tgcgcccggc cgaaatcgcc agactgagca
     4861 aatcccaagg agacactttt catgagctct gcccgccgcc cactgcttgc cgccatcgtc
     4921 tgcgctgtcg cgctgggcgg ctttgccggc accgctggtg ccaacagctt tccgtccaag
     4981 ccggtgtcca tcatggtgcc gtaccccgca ggcggcatct ccgacaccat cgcgcggcgc
     5041 gtcaacgtgc cgctgggtca ggcgctgggc cagacggtga tcgtcgagaa cctgggcggc
     5101 gccagcggct cgatcgcggc gcagaaggtg ctgaacgcgc cggccgacgg ctacaacatg
     5161 ttccagggct cgcccaatga gctgatcctg gcgccgctgg ccatccccgg catcaaatac
     5221 aagccggaag acttccgcca ggtgcaccgc atctcgctgg gcccgatggc catctttgcc
     5281 cgcgcgaatc tgccggccgg caatgccgat gaactgtcgg cttacgcggc caaggtggcc
     5341 aaggagggca aacccatgac ctacgccagc gtcggcgtcg gctccttcta ccacctgctg
     5401 ggcggcaaga tgagcaagga catcggcgtc gagatgaccc acgtgcccta caagggcgcg
     5461 gccgacgtgg cgcgcgatct ggcgggcggt caggtggaca tcttcatcac gccctacggc
     5521 ccttcgctgg tggagctggt caagcagggc aagctcaagg tcgtggccgc tttgtcgacg
     5581 cagcgccagc cggccattcc tgatgtgccg accacggcgg aaagcaaggc gctgaagaac
     5641 ttccagtacc agatcggcac cggctatttc gtcaagcgct ccacgccgga gccggtggtg
     5701 cagacgctgc acaagggtct gcagaaggtg ctgagcgatg ccgaagtcaa ggccgccctg
     5761 gaagccaacg gcgcccaggt gtcgccgccg cagacgctgg ccgaggccga cgccgccttc
     5821 aagcaggaga ccgccaccta ccaggcgatc gccaggagca tcgggctggt gcagccatga
     5881 gcagcgccgt cgctcaagcg ccgcacgcgc tgctgacgca cctgcagggt ggtgccgagg
     5941 ccttcgtcgc gctgcgccgc gacatccatc ggcaccccga gctgggctac caggaggtgc
     6001 gcaccagcga cctggtggcc gagcgcctga ccgcctgggg ctaccaggtc acgcgcggcc
     6061 tgggcggcac cggcctggtc gggcaactgc ggcgcggcag cggccaaaag cgcctgggcc
     6121 tgcgcgccga catggacgcg ctgccgatcc aggaggccac cggactgccc tgggccagcc
     6181 agcacgccgg ccgcatgcac gcctgcggcc acgacggcca caccgccatg ctgctggcgg
     6241 cggcgcacca cattgccgag cacgggcgct tttcgggcac gctaaacctg atcttccagc
     6301 cggccgaaga aggcctgggc ggcgccaagg cgatgatgga cgacggcctg ttcgagcgct
     6361 ttccctgcga cgccatcttc gccatgcaca acatgccggg ctatccgcag ggcaagctgc
     6421 tgctgcgcga aggcccgctg atggcctcgt ccgacggtct gaccatcacc ctcaccggca
     6481 agggcagcca cgccgccgtg ccgcagcacg gcatcgaccc ggtggtggcg ggcagcagca
     6541 tcgtgctggg cctgcaatcc atcgtggcgc gcaacgtcga tccacaggcc atggcggtga
     6601 tcaccgtcgg cgccttccag gccggccacg cgcacaacgt gattccgggc gaggcggtgc
     6661 tcaagctcag cgtgcgcgcg ctcgaccgca aggtgcgcgc caccctgctg cggcgcatcg
     6721 aggaactggt ggacgcgcag gccagggcct ttggcgtcgg ttggcgcatg gagagggtca
     6781 ccggctaccc ggtgctggtc aatacccggg ccgagaccga gttcgcccgc cgggtggcca
     6841 ccgagctggt cggcgcggcg cgcgtcgagc cgcagaccgt ggcgctgtcg ggcagcgagg
     6901 acttcgcctt catgctcgaa caggtgcccg gcagctacct gctgatcggc aatggcgacg
     6961 gcagcgacga cggcgccaac ggtggccatg gcgcctgcat ggtgcacaac cccggctacg
     7021 acttcaacga cgccaacctg ccggtcggtg cggcctattg ggcgctgctc gtcgagcgct
     7081 ttctgagcga cgcggcctga cggcgttcag cgtcaaattg gcctctggcg cgcgccggac
     7141 gtgcgccgac agctatcaat gatatagcat tcagaccagc aggccggccg ccagacggtg
     7201 cagccggctg cgcggcgcag ccagggtgtc gaggatgctg aagtggttca accccggcag
     7261 ctcggccgcc accggcaccc gttcgcggcc ccaggcctgc tggatcagct tgttcaggcg
     7321 caggtattcg ccgctttcct cgcctcccac cacgctgatc agccgcccgt gcgcgggcgc
     7381 cggcaggcgc gccgggctgg ccacctggac ctggcgcggc gtcaggcgca gcacctcctg
     7441 caggctgggc gtgtgcagca gcggctgcag gtcgtacagg cccgagatgc cgagcgccgc
     7501 acgcaccagg cgcggcggca gggcggggtc gaaggtgctc caggcgcagc ccagcagcat
     7561 ggccgccagt tggccgccgg cggaatggcc gatcaccacg aggcggcgcg cgtcaccgcg
     7621 cagcgcgctc gcgtggcgcc cgcaccaggc cacggcgcgc accatctgca gcgtgatgtc
     7681 ggggatgctg gccttgggcg ccagcgtgta gttgggcacc accaccgccg cccccagcgc
     7741 gcgcagcgcc ggcaccacaa agctgtgctg cgacttgtcc atcgccttcc agtagccgcc
     7801 gtggatgaac accacgatgg gcgagcgcgg gcgctcggcc gggaaggcgt ccagcgtctc
     7861 gcctgcggcg tcgccatagg gcagatcgag ctggcagggc aggctgcgcc gcaccagcgc
     7921 cgactcggcc gcccagcggg caaagtaatc gccgtggtca ggcacccgcc ggaggttgtt
     7981 gtacatgcgc tccagccact gggtgtcggg ttggtcgcgg cggctggaat cgttggggcg
     8041 ggccatggtg gagcgctatt gtcgcaaacc ggccggccgc cgcgcgctgc cgtgcgccag
     8101 actgtgtctt gctgcctaca atcggcttcg acgcctgcgg ggtgagcgca ggtagctaca
     8161 aaacaaggag cattcgagat gacgctgacg ctgctgggta ccggtcgcat gggcttgccg
     8221 atggcacgcc ggctgtgtca ggcaggccat gagctgcacg tgtggaaccg cagcatcgac
     8281 aaggcgcagc cgctggcggc cgacggcgcc accgtgcatg gcgatctggc ctccgccgcg
     8341 gcggccggcg acatcgtcat cagcctgctg gagaacggcc cggtggtggc cagcgtgctg
     8401 ttcgagcagg gcgcggcggc ggccctgcgg ccgggcagcc tgttcatcga catggcttcc
     8461 atcaagccgg ccgaagcgcg cgagcacgcc gcgcggctgg ccgagcgcgg cgtgcgccac
     8521 ctggacgcgc cggtgtccgg cggcaccgtg ggcgccgagg ccggcacgct ggccatcatg
     8581 gtcggcggcg aggtgcagga ctttgaacgt gctcggccgg tgttcgatgt gctgggccgc
     8641 gccacccacg tcggcccgca tggcgccggg cagctggcca agctggccaa ccagatgatc
     8701 gtcggcatca ccatcggtgc cgtggccgag gcgctgttgc tgtgccagaa gggcggcgcc
     8761 gacccggcca aggtgcgcca ggccatcggc ggtggctttg cgcaaagccg cattctggag
     8821 ctgcatggcg agcgcatggt cacgcgcgac ttcgcgccgc gcgcgcgcat gacggtgcag
     8881 ctcaaggacc tgcgcaatgc gctggacacc gcccaggcca tgggcttcga agcgcccgtc
     8941 accgccgcgc tggagcgcct gtacgccgac ggcattgccc acggcgacag cgagctggac
     9001 catgccggcc tgttcgtcga gctggcgcgg cgcaacggca tgagctgaac gtcgcgccgg
     9061 ccgcggcggc gcgcatggca gggcgggccg ggtgttacaa ccacggcttt gcatcacgga
     9121 ttgaccatga gtctgtacgc tcaactgcag cagcgcgccg ccgccggccg cccgatccgc
     9181 gtcggcctga tcggcgccgg caaattcggc tccatgtacc tggcgcaggt gccgcgcacg
     9241 ccgggcgtgc acctggcggc cattgccgat ctgtcgcccg ccgccgcccg cgccaacctg
     9301 gcgcgcgtgg gctggcggcc cgagcaggcg caggccgcgt ccatcgatga ggcgctgcag
     9361 cgcggcagca cccacgtggg cgaggactgg caggccctag tgcgccaccc ggcgatcgac
     9421 gtggtggtcg aatgcaccgg ccacccgatc gccgcggtcg accactgcct ggccgccttc
     9481 gacgcgggca agcacgtggt caacgtcacc gtcgaggccg acgccttctg cggcccgctg
     9541 ctggcgcggc gcgcggccga gtccggtgtg gtctattcgc tcgcctttgg cgaccagccg
     9601 gcgctgatct gcgacctggt ggactgggcg cgcacctgtg gctttccggt ggtcgccgcc
     9661 gggcgcggcc acaaatggct gccgcacttc tgcgagagca cgcccgaaac cgtgtggggc
     9721 tactacggat tgagccccga gcaggccgcg cgcggcggcc tgaaccccaa gatgttcaac
     9781 agctttctgg acggcagcaa gccggccatc gaaagcacgg cggtcgccaa cgccaccggg
     9841 ctggcggtgc cgacgggcgg acttcagtat ccgagcggca gcattgccga catccccagg
     9901 ctgacgcgcc cgcgcagcgc aggcggcgtg ctggaaaaga aaggcatggt cgaggtcatc
     9961 tccagcctgc gccccgacgg cacgccgatc gactacgaca tccgcatggg cgtgtgggtg
    10021 acggtggagg ccgagaccga ctacatccgc cactgcttcg aggaatacaa cgcccacacc
    10081 gacgacagcg gccgctactt caccctgtac aagcgctggc acctgatcgg cctggaggtc
    10141 ggcctgagcg tggccagcgt ggcgctgcgc cgcgaagcca ccggcgtggc gcagggctgg
    10201 cacgccgacg tggtggccac cgccaagcgc gacctcaagc ccggcgacgt gctcgatggc
    10261 gaaggcggca ccaccgtatg gggcaagctg cagcccgctt caaggtcggt ggccatgggc
    10321 ggcctgccgc tgggcctggc gcacgacgtg aaggtgctgc gcccggtcac caagggccag
    10381 tgcctgagct gggacgacgt cgccatggac acgcgcacgc gcgcctggca actgcgccag
    10441 cagatgcagc agctgctcac gccggccgac tgaattgcgc taaaatggcc gtccttcgtg
    10501 ggggggtagc tcagctggga gagcgccgcg ttcgcaatgc ggaggtcggg agttcgatcc
    10561 tcctcctctc caccaccatt cagaagccag gccacgtgcc tggctcttcg ttttcggcca
    10621 gtgc
//
LOCUS       DAOOOG010000084        10601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_7085_length_10601_cov_27.097098, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000084 DAOOOG010000000
VERSION     DAOOOG010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10601
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7085_length_10601_cov_27.097098"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PK242_07660"
     CDS             <1..1082
                     /locus_tag="PK242_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOZ93930.1"
                     /translation="QPVRVSGKCGAWLSDVVEGIRWAAGLPVSGVPTNPTPARVINVS
                     FGGSSSCNSAYQTAIDDAGAAGSLVVVAAGNENGALTRPADCAGVLAVGAARQDGLKT
                     YYSSYGPNVGIMAPGGPGAADTGPRLYSTSNAGLRGPGEHIYDSKDGTSFASPLAAGV
                     AALMLSVNPALTPAQLISRIQSGARPFPSSAAYPTCRVGMPASSACNCTRETCGPGLL
                     DADRALQLATSPSAVIAPIGSVPAGSTVTLDGRGSAAVAGASIASYQWSQVSGSPVGI
                     RDAGAPVASVSLPDARGDWVFRLLVTDTLGRSADNYVTVSTYRDGDDDEGGGATGLAW
                     GAGLWALALGALWHRRTRRRTAATS"
     gene            complement(1285..1986)
                     /locus_tag="PK242_07665"
     CDS             complement(1285..1986)
                     /locus_tag="PK242_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOZ93931.1"
                     /translation="MSDTPTPAHLAPTPAIDCDHPAVQALARQHAQGASERQRAVALT
                     HAVRDAFRYDPYRIDLSPAGMRASTVIAHGYGWCVPKAVLLAAAARAAGIPARLGFAD
                     VRNHLSTERMRQSMQTDLFIWHGYTELWLDGAWRKATPAFNLSLCQKFNLLPLDFDGV
                     HDSIYHPFDASGNRHMEYVRQRGSFDDLPLDRIVADFDRVYGPWLRRGTDSALGEGDF
                     LADVARETGAPSERA"
     gene            complement(2213..3094)
                     /locus_tag="PK242_07670"
     CDS             complement(2213..3094)
                     /locus_tag="PK242_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ93932.1"
                     /translation="MNDLDWNWLKAFLHTAQSGSLSAAARKLGLTQPTLSRQVAAIER
                     QMGVTLFERVGKSMVPTPTGLDLLEHARAMGNAAEALGLAANGRSQAVGGVVTVSASD
                     AVAAHLLPVIVRRLRAQEPGIAIEVISSNAVSDLLRREADIAIRHVKPEQPELIARLV
                     REAQALFYASESWVQTYGHPRTATDAAGLPFVGSDRNGRYLAYLRDHGLNLSEDSFSC
                     YADHSVTHWALVRQGLGIGAMMDEIARETPGMVRVLDDVAPVRFPIWLVTHRELRTSR
                     RIRVVFEALAEGLANGH"
     gene            3148..3921
                     /locus_tag="PK242_07675"
     CDS             3148..3921
                     /locus_tag="PK242_07675"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ93933.1"
                     /translation="MDSIWGLLVANACDHHFGMSYGLHPMLLPPNTTVRDTSPSAASR
                     SASASKARFWDRIAPKYAADPIADMAGYETTLRRAQELLSPQQDVLEVGCGTGHTALR
                     LAPFTRRLMATDVSAEMIAIACLRQAAQPSPQLTFALADAEATDFGRATYDAVLAFNV
                     LHLTSNLGDALKPLVHALRPGGLLISKTPCIAEMHPWIPYLAVPLMRAIGKAPPVLCF
                     DAQRLQLTMVQQGLQIVCAERHGTRGKDTRVFIVARKPG"
     gene            4070..4639
                     /locus_tag="PK242_07680"
     CDS             4070..4639
                     /locus_tag="PK242_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93934.1"
                     /translation="MSLFSPTLHSTNRSGWSGNVLIAMAIVSLTACGGGSDGESGPPD
                     GGSGGTGPSVSIGALEGDWLQKGCVKAGAQSYKRTLRVRITGQTIVDYFEGVLTFRGN
                     DCAGASQAAGPTRTGTVTFARSEGSQVLTAHWGELRTVIGTRSGAIWTLQPQDLLCIL
                     GDEIPTIQPTLTAVSSSVATIPVDNCFGR"
     gene            4680..5105
                     /locus_tag="PK242_07685"
     CDS             4680..5105
                     /locus_tag="PK242_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93935.1"
                     /translation="MKPRMRLLSIAPMACVVAAQVLLPGCASTLDQAGLEQRTAQAIG
                     RSAGQFTITDQQEEIGGRVSYTVSTSEGATYRCYLYGATGFQKAMSFGQTPHSDAICT
                     DMVGSKPSSKHQAPATAPEQPGRNRAKACNALQRTAGRC"
     gene            5136..5990
                     /locus_tag="PK242_07690"
     CDS             5136..5990
                     /locus_tag="PK242_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ93936.1"
                     /translation="MFNLLKPDQWGKWPCVVLVLTLGLCGCTTVPGAAIETVDGRKVE
                     YAQSGQGTPVVVFENGLGATLDWWAKVWPDAVAEARSLAYNRAGYGHSDTSLAPRDGA
                     QVVDELRALLQARNLPPPYVLVGHSLGGLYMQLFARKFPDEVAALVLVDSTHPDQFKG
                     AGNPDQWPTWLKVVLHATTSETAKQELAALDATGEQVSGLPVAPSMPVWVLSALRATG
                     TPSALTDDADRKRSNIARLYPGSVQIWVNSGHAIPLEKPGAVVSAIRAAARATRASGA
                     ARMEPQDR"
     gene            6061..6387
                     /locus_tag="PK242_07695"
     CDS             6061..6387
                     /locus_tag="PK242_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93937.1"
                     /translation="MLYEWMKLRTLAAGAGHDSQFEQYLRPSKPGMFLATTEQIVPWP
                     ELCAVTEPHYPMPANGRPPAELERMLRTCLAQLRISPVDEPCEEALMDGTLVQARGLK
                     AGTPVP"
     gene            complement(6534..7214)
                     /locus_tag="PK242_07700"
     CDS             complement(6534..7214)
                     /locus_tag="PK242_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOZ93938.1"
                     /translation="MRFEAILFDCDGVLVDSEPITHGVLRQQLAESGWDMPLAECMRH
                     FMGRTVRSQAALIEARTGRPLTDAWMARFYARRDAQLRQHLQPIDGAPDAVRAAHALT
                     GGRIACASGADRGKLVLQLAHTGLAPWFGPHVFSGHELPRSKPFPDVYLAAAAALGAA
                     PRRCLVIEDTPTGIQAGVAAGATVWALCPQPEAAPALREAGAHALFGAMAELPRLWRA
                     GQAPAWAE"
     gene            complement(7331..8791)
                     /locus_tag="PK242_07705"
     CDS             complement(7331..8791)
                     /locus_tag="PK242_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOZ93939.1"
                     /translation="MRRHVHVLRVLAWLIMGFSLAFLVPAAWTLVDARYRQAEIWLLA
                     GAGTAACGALLWAATRRFRRELSVKDGFLLVNLVWTVLPGFAAAPLLLTVPGISVTDA
                     YFEAMSGLTATGATALAGLDLLDPAVNMWRCFLQLIGGLGIMLLAVAVLPFLGLGGMQ
                     LFKAEMPGPMKDTRLTPRIAETARGLWGVYFVLSLACMLAYRWGGMSWDDAFMHMCTT
                     VGLGGLSSHDQSFGYWNSPALEWLAVLFMALSGISFARYFMVWRTRSVRPLWRDAEVR
                     AYVVVLLVSVAAVWLLLLREGKHVDAGHALRDAAFQVVSVATTTGYATTDYLLWPTIG
                     PVLMLLLGAIVSCAGSTGGGIKMVRVLILAKVTLHELVQLIHPRVVNPVTLGGQTVPA
                     RVIGAVIAYMLVYGAVLVGLSLLMLASGLDVVTAVSAIIACLNNIGPGMGKVGPAGNY
                     AVLTDFQIWLCSLAMMLGRLELLSVLVLFTPQFWRR"
     gene            complement(8815..10203)
                     /gene="trkA"
                     /locus_tag="PK242_07710"
     CDS             complement(8815..10203)
                     /gene="trkA"
                     /locus_tag="PK242_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOZ93940.1"
                     /translation="MKILILGAGRVGQSVAETLASEANDITIIDPDPERLRGLEERLE
                     LRGVAGNGVQPSVLRHAGADDCDLFIACADLDATNLVACKVAHDVFGIPTTIARLRLP
                     DFRQDDRLLGPEGFAVSRVICPEASVTRTIQELIEHPEALQVLQFSQGRAHLVAARVA
                     RGSALDGRSIADFIAQVPEVPMRLVAVYRRDEETPCEADTRVLAGDEVFVLVAREHVQ
                     AALAAIGPREPPVRRVMIAGGGKVGLGVARSLIGQCEVKLIEKQRGRCRYLAGELPGD
                     MLVLQGDAADEELLAAEHVGDMDLFISLTSDDEDNIMSAMLAKRMGARRVMALINRRA
                     YAEMMQASPIDIIISPADAVIGELLAHVRRGAVAAVHSLRRGVAEALEGVAHGDARTS
                     RLVGRRIEQLALPAGARVGAIVRGEGDAGQVLVPHHDTVIEEGDHVIIFVPHKRLLPQ
                     VEKLFQVRATFF"
     gene            complement(10290..>10601)
                     /locus_tag="PK242_07715"
     CDS             complement(10290..>10601)
                     /locus_tag="PK242_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HOZ93941.1"
                     /translation="APDAEALMRSRYTAFVRGDADYLLSSWHASQRPATLALEPHIRW
                     LGLEVRAHRVLDETHAEVEFVARSRVAGGRATRLHERSRFVRENVQGGWRWFYLDGDL
                     S"
BASE COUNT         1765 a         3646 c         3524 g         1666 t
ORIGIN      
        1 tgcagccggt gcgcgtgtcg ggcaagtgcg gcgcctggct gtcggacgtg gtggagggca
       61 ttcgctgggc cgccggcctg ccggtcagcg gcgtgcccac caacccgacg ccggcacggg
      121 tgatcaacgt cagctttggc ggcagctcca gctgcaactc ggcctaccag acggccatcg
      181 acgacgccgg cgccgccggc agcctggtgg tggtggcggc cggcaacgaa aacggcgccc
      241 tgacgcggcc ggccgactgt gcgggcgtgc tggcggtggg cgcggcacgt caggacgggc
      301 tgaagaccta ctactccagc tacggcccca acgtcggcat catggcgccg ggcggcccgg
      361 gcgccgccga caccggcccg cggctgtatt ccacctccaa cgccggcctg cgcgggccgg
      421 gcgagcacat ctacgacagc aaggacggca ccagttttgc gtcgccgctg gcggccggcg
      481 tggcggcgct gatgctgtcg gtcaacccgg cgctgacgcc ggcgcagctg atctcgcgca
      541 tccagtcggg cgcgcggccg ttccccagca gcgcggccta ccccacctgc cgcgtcggca
      601 tgccggccag cagcgcctgc aactgcacgc gcgagacctg cggtcccggc ctgctggacg
      661 ccgaccgcgc gctgcaactg gccaccagcc cgtcggcggt gatcgcgccg atcggcagcg
      721 tgccggccgg cagcaccgtc acgctggacg gccggggcag cgcggcggtg gccggcgcca
      781 gcatcgccag ctaccagtgg agccaggtct cgggtagccc ggtcggcatc cgcgacgccg
      841 gcgcgccggt ggccagcgtc agcctgcccg acgcgcgcgg cgactgggtg ttccgcctgc
      901 tggtgaccga cacgctgggc cgctcggccg acaactacgt gaccgtgagc acctaccgcg
      961 atggcgatga tgatgaaggc ggcggcgcca ccggcctggc ctggggcgcc ggcctgtggg
     1021 cgctggcgct gggggcgctg tggcaccgcc gcacccgccg ccgcacggcc gccaccagct
     1081 gatgcgggcg ccggccatac tattgttttg atagctgttg gcgcttgcca gacaagcgcc
     1141 ggagcctgat ctgaccaaga acggtcaggg acttcccgcg cacccgcgtt cccaatggcc
     1201 catgccggat gagctcgctg cgcttcgatg accgatgggt tcggcgcagc cggtgtcatg
     1261 ggacgttttt agcgtgaaac ggtatcaggc gcgctcggac ggtgcgccgg tctcgcgcgc
     1321 cacgtcggcc aggaagtcgc cctcgcccag cgcgctgtcg gtgccacggc gcagccacgg
     1381 gccgtagacg cggtcgaagt cggccacgat gcgatcgagc ggcaggtcgt cgaagctgcc
     1441 gcgctgccgg acgtattcca tgtgccggtt gccgctggcg tcgaacgggt ggtagatcga
     1501 gtcgtgcacg ccgtcgaagt cgagcggcag caggttgaat ttctgacaca ggctgaggtt
     1561 gaacgccggc gtggccttgc gccaggcgcc gtccagccac agctcggtgt agccgtgcca
     1621 gatgaacagg tcggtctgca tgctctggcg catgcgctcg gtgctcaggt ggttgcgcac
     1681 gtcggcaaag cccagccgcg ccgggatgcc ggcggcgcgc gccgccgccg ccagcagcac
     1741 cgccttgggc acgcaccagc cgtagccgtg ggcgatcacg gtgctggcgc gcatgccggc
     1801 gggcgacagg tcgatccggt aggggtcgta gcggaaggcg tcgcgcacgg cgtgcgtcag
     1861 cgccacggcg cgctggcgct cgctggcgcc ttgcgcgtgc tggcgcgcca gggcctgcac
     1921 cgccgggtgg tcgcagtcga tggcgggcgt gggcgccagg tgggcggggg tgggggtgtc
     1981 gctcatggtt cccatcttag ggcgacactg aaccagtcct tctcgcgccg cgcatccctg
     2041 gtgtgaaagg tctgcggtgt tgcaactgct cgtcatcacc atggctgtgg gtgggctgat
     2101 ggagcttgcc atccatcctc aatcatggcg aagggcgcgc gccatcgcag ggacttgttc
     2161 agcgtcgcct tggtgcgcgt ggttttctct cgcaccactc ggattggcgt cgtcagtgac
     2221 cattggccag tccctctgcc agcgcctcaa aaaccacccg aatgcgccgg gatgtgcgca
     2281 gctctcgatg cgtcaccagc cagatcggga accttacggg cgccacatcg tccagcaccc
     2341 ggaccatgcc cggggtctct cgagcaatct cgtccatcat cgcaccgatg cccaagccct
     2401 ggcgcaccag agcccaatgg gtcacactgt gatccgcgta gcagctgaag ctgtcttcgc
     2461 tcagattcag cccatgatcg cgcaggtatg cgagataccg gccattgcga tccgacccca
     2521 caaacggcag cccggcagcg tccgttgcag tgcggggatg gccgtacgtc tgaacccaag
     2581 attcagacgc ataaaaaagc gcctgggctt cgcgcaccag gcgggcgatc aactcaggct
     2641 gttcaggctt gacgtggcgg atggcgatgt cggcctcgcg gcgcagcagg tcgctcacgg
     2701 cgttggatga aatcacctcg atcgcaatgc ccggctcctg ggctcgcaag cgccgcacga
     2761 tgacaggaag gagatgggcc gccacggcat cgctggccga caccgtcacc acgccgccca
     2821 ccgcttgcga cctgccgttg gcggccaagc ccagggcctc tgccgcgttg cccatggcac
     2881 gggcgtgctc cagcagatcc agccctgtgg gtgttggcac catggacttg cccacgcgct
     2941 cgaacagggt cacccccatt tgccgctcga tggctgccac ctgccgactc agcgtgggct
     3001 gggtcaagcc cagcttgcga gcggcagcag acagggagcc gctctgcgcg gtgtgcagaa
     3061 aggccttcag ccaattccag tcaaggtcat tcatacataa atgcatgtat ccactgcaat
     3121 tcaaagccat tttctacgct tcattgcatg gatagcatct gggggttgct cgttgcaaat
     3181 gcctgcgatc accacttcgg catgtcctac ggtctgcacc ctatgctctt gcctcccaat
     3241 accacggtcc gtgacacctc accttcggcc gcatcccgtt cggcttctgc gtccaaggca
     3301 cggttttggg accgcatcgc ccccaagtac gcagcggacc ccattgccga catggcgggg
     3361 tatgaaacca cattgcgccg cgcgcaggag ctgctttccc cccaacaaga tgtgctggaa
     3421 gttggttgcg gaacgggcca tacggcgctg cgcctggctc ccttcacccg gcgtttgatg
     3481 gccacggatg tgtcggcgga gatgattgcc attgcctgtc tgcggcaagc tgcacaaccc
     3541 tcgccgcaac tcacgtttgc cctggccgat gccgaggcca ccgactttgg gcgagccacc
     3601 tatgacgcgg tgctggcctt caatgtgctg cacctgacga gcaatctggg tgatgcactg
     3661 aagccgttgg tgcatgcatt gcggccaggt ggactgttga tttccaaaac gccctgcatt
     3721 gccgaaatgc acccttggat tccttacttg gctgtaccgt tgatgcgggc cattggaaaa
     3781 gcgccgcccg tactgtgctt tgacgcgcaa cggctgcaat tgactatggt gcagcagggt
     3841 ctgcagatcg tttgcgccga gcggcacggt acgcggggca aggatacccg cgtctttatc
     3901 gtggcccgca agccgggctg aaccgccccc agtcacgcgc atgtggcccg ttgcaaggcc
     3961 gcaggggcag ttttgccttc tgggtttctg ctgcgctgtt gccgtcatct ttcctggcca
     4021 ccactcaacc ccggttgaca tttctctgcc gatccccaag gagcccgtca tgtctctctt
     4081 ctcccctacg ctgcactcca ccaaccgatc tggctggtct ggcaatgtgc tgatcgccat
     4141 ggccatcgtt tcactgacag cttgcggtgg tggctcggac ggcgagagtg gcccaccaga
     4201 cggcggttcg ggtggcacgg ggccttccgt gtcgatcggc gcattggagg gggattggtt
     4261 gcagaaagga tgcgtgaagg ccggcgcaca atcctacaag cggaccctgc gcgtgcgcat
     4321 cacaggccag accatcgttg actatttcga aggcgtgctg accttccgtg gcaatgactg
     4381 tgctggcgca tcgcaagctg ctgggccaac cagaaccggc acggtgactt ttgcccgctc
     4441 ggaaggttcc caagtactga ccgctcattg gggcgagtta cgcaccgtca tcggcacccg
     4501 ctcaggagcc atctggacgc tgcaacccca agacctgctg tgcatattgg gcgacgagat
     4561 tccaaccatt cagcccacgc tcacagccgt gtcctccagt gtggccacca tccctgtaga
     4621 caactgcttt ggccgctgac accgcccacg ccatgacatc cattgcatcc aacactgcca
     4681 tgaaaccgcg catgcgcctg ctgtccatcg cccctatggc ctgcgttgtc gcagcccagg
     4741 tgctgctccc gggttgcgcc tccacgcttg atcaggcagg tctggagcag cgcacggcgc
     4801 aagccatagg gcgttctgcc ggccagttca ccatcacaga ccaacaggag gaaattggtg
     4861 gccgcgtcag ctacaccgtc agcaccagtg agggcgcaac ctaccggtgc tatctctacg
     4921 gcgccacggg tttccaaaag gccatgagct ttggtcaaac gccccattcc gatgcgatat
     4981 gcaccgacat ggtcggcagc aagcccagca gcaaacacca ggcaccagcg accgcaccag
     5041 agcagccggg acgcaaccgc gccaaggcat gcaatgctct gcagcgcact gcggggcgtt
     5101 gctgaatacc gctgtgctga cactcgatcc gatccatgtt caaccttctg aaaccggatc
     5161 aatgggggaa atggccttgc gtcgtcctgg tactgacact gggcttatgc ggctgcacga
     5221 cagtgccggg cgccgcgatc gaaacggtgg acgggcgcaa ggtggagtac gcgcaatctg
     5281 ggcagggcac accggtggtc gtgtttgaga acgggctggg tgccacgctc gactggtggg
     5341 caaaggtttg gcctgatgca gtggccgaag cacgttcctt ggcctataac cgggccggtt
     5401 atggccacag cgatacgtcc ctggctccgc gtgacggcgc tcaagtagtg gatgagttgc
     5461 gagcattgct gcaggcgcgc aatctgccgc caccgtatgt tcttgtggga cattctctgg
     5521 gcggcctgta catgcaactg ttcgcacgca aattccccga cgaggtggcg gcgctcgtgc
     5581 tggtggactc cactcatcct gatcagttca agggtgctgg caacccggat cagtggccca
     5641 cttggctgaa agtggttctc cacgcaacga catccgaaac ggcaaagcag gaacttgcgg
     5701 cactggatgc cacaggagag caagtatccg gcttgccagt ggctccctcg atgcccgtat
     5761 gggtgctgag tgcgctgcgc gccacaggaa cgccttcagc gctgacggac gacgcggaca
     5821 gaaagcggtc caacattgcc cgtctgtacc ccggctctgt ccagatatgg gtgaacagcg
     5881 gccacgctat tcctctggaa aaacccgggg ccgtggtcag cgcgatacgg gctgcggcgc
     5941 gcgcgacgcg cgcatccggc gctgcgcgca tggaaccgca agatcggtag cctacagtcg
     6001 aggcaagggt gccctgcatt gtgagggcca cgctgaaaaa gtacatcact gaccggccag
     6061 atgctttatg agtggatgaa gcttcggacc ctcgccgcag gtgccggcca cgacagccag
     6121 ttcgagcaat acctccgccc gagcaagccc ggcatgttcc tggcgacgac ggaacagatc
     6181 gtgccatggc cggagctgtg cgcagtcacc gagccgcact accccatgcc ggccaatgga
     6241 cgcccgccgg cagagctcga gcgcatgctg cgcacgtgcc ttgcacagct ccggatcagc
     6301 ccggtcgatg agccttgcga ggaggcgctg atggacggca cgctggtaca ggcgcgaggg
     6361 ctgaaggcgg gcacacccgt gccgtgatcg gcttgcccgg cagcatgaat gcggcctgcc
     6421 cgaagttgac aagcggtcaa ctgccgtttc tggggtgagc cgttcaccct gagcctatcg
     6481 aagggcgtcg gcaggctcag cccgaacggt attcaagacc tccaaaggcc gaatcattcg
     6541 gcccaggccg gcgcctgccc ggcacgccac agccgcggta gctcggccat ggcaccgaac
     6601 agcgcgtgcg cgcccgcctc gcgcagcgcc ggcgccgcct cgggctgcgg gcacaaggcc
     6661 cagacggtgg cgcccgcggc cacgccggcc tggatgccgg tgggcgtgtc ctcgatcacc
     6721 aggcagcgcc ggggcgccgc gcccagcgcc gcggcggcgg ccaggtagac gtccggaaag
     6781 ggcttgctgc gcggcagctc gtggccgctg aacacgtgcg ggccgaacca tggcgccagg
     6841 ccggtgtggg ccagttgcag caccagcttg ccgcggtcgg cgcccgaggc gcaggcgatg
     6901 cgcccgccgg tcagcgcgtg ggcggcgcgc acggcgtcgg gcgcgccgtc gatgggctgc
     6961 aggtgctggc gcaactgggc gtcgcgccgg gcgtagaagc gggccatcca ggcgtcggtg
     7021 agcggccggc cggtgcgcgc ctcgatcagc gccgcctggc tgcgcacggt gcgccccatg
     7081 aagtgccgca tgcactcggc cagcggcatg tcccagccgc tttcggccag ttgctggcgc
     7141 agcacgccgt gggtgattgg ctcgctgtcc accagcacgc cgtcgcagtc gaacagaatc
     7201 gcttcaaatc tcatagctgt ttgcgcacgt ccatggcggg ttggatcgac atttctttca
     7261 tggtccggag gctcgaacgg cgtgcgcagc ccgtccaccc agggaagcgg cgcagcggct
     7321 ctggggccct ctacctgcgc cagaactgcg gcgtgaacag caccagcacc gacagcagct
     7381 ccagccgccc cagcatcatg gccaggctgc acagccagat ctggaaatcg gtcagcaccg
     7441 cgtagttgcc ggccggcccc accttgccca tgccggggcc gatgttgttc aggcaggcga
     7501 tgatggccga caccgcggtc accacgtcca gcccgctggc cagcatcagc agcgacagcc
     7561 cgaccagcac ggcgccatag accagcatgt aggcgatgac ggcgccgatc acgcgcgccg
     7621 gtaccgtctg gccaccgagc gtgaccgggt tgaccacgcg cgggtggatc agctgcacca
     7681 gctcgtgcag cgtcaccttg gccaggatca gcacgcgcac catcttgatg ccgccaccgg
     7741 tagagccggc gcaggagacg atggcgccca gcagcagcat cagcaccggg ccgatggtgg
     7801 gccacagcag gtagtcggtg gtggcgtagc cggtggtggt ggccaccgac accacctgga
     7861 acgccgcgtc gcgcagcgca tggccggcgt ccacatgttt gccctcgcgc agcagcagca
     7921 gccagaccgc ggcgacggac accagcagca ccacgacgta ggcgcgcact tcggcgtcgc
     7981 gccacaacgg tcgcaccgag cgcgtgcgcc agaccatgaa gtagcgcgca aagctgatgc
     8041 ccgacagcgc catgaacagc accgccagcc actccagcgc gggcgagttc cagtagccga
     8101 agctctggtc gtgcgaagac agcccgccca gccccaccgt ggtgcacatg tgcatgaagg
     8161 cgtcgtccca gctcatgcca ccccagcggt aggccagcat gcaggccagc gacagcacga
     8221 agtacacgcc ccacaggccg cgcgcggttt cggcgatgcg cggcgtcagc cgggtgtcct
     8281 tcatggggcc gggcatttcg gccttgaaca gctgcatgcc gcccagcccc agaaacggca
     8341 gcaccgccac cgccagcagc atgatgccca ggccgccgat cagctgcaga aagcagcgcc
     8401 acatgttgac cgccgggtcg agcagatcca gccccgccag cgcggtggcg ccggtggcgg
     8461 tcaggccgct catggcctcg aagtaggcgt cggtgacgct gatgccgggc acggtcagca
     8521 gcagcggtgc ggcggcaaag cccggcagca ccgtccacac cagattgacc agcagaaaac
     8581 cgtccttgac cgacagctcg cgccggaagc gccgcgtggc cgcccacagc agcgcgccac
     8641 aggccgccgt gccggcaccg gcgagcagcc agatctcggc ctggcggtaa cgcgcgtcca
     8701 ccagcgtcca ggccgccggc accagaaacg ccagagaaaa acccatgatc agccacgcca
     8761 gcacccgcag cacgtgcacg tggcgacgca tcagcatccg catgtccgca gctttcagaa
     8821 gaaggtggcg cgcacctgaa acagcttttc cacctgcggc agcagacgct tgtggggcac
     8881 gaagatgatg acgtggtcgc cctcctcgat caccgtgtcg tggtgcggca ccagcacctg
     8941 gccggcgtcg ccctcgccgc gcacgatggc gcccacgcgt gcgccggccg gcagcgccag
     9001 ctgctcgatg cgccgcccca ccaggcgcga ggtgcgcgca tcgccatggg ccacgccctc
     9061 cagcgcctcg gccacgccgc ggcgcaggct gtgcaccgcc gccaccgcgc cgcgccgcac
     9121 atgcgccagc agctcgccga tcaccgcgtc ggcgggcgag atgatgatgt cgatggggct
     9181 ggcctgcatc atctcggcgt aggcgcggcg gttgatcagc gccatcacgc ggcgcgcgcc
     9241 catgcgcttg gccagcatgg cggacatgat gttgtcctcg tcgtcgctgg tcagcgagat
     9301 gaacaggtcc atgtcgccca cgtgctcggc ggccagcagc tcctcgtcgg cggcgtcgcc
     9361 ctgcagcacc agcatgtcgc cgggcagctc gccggccagg tagcggcagc ggccgcgctg
     9421 cttctcgatc agcttgacct cgcactggcc gatcagcgag cgcgccacgc ccagcccgac
     9481 cttgccgccg ccggcgatca tcacgcgcct gaccggcggc tcgcgcgggc cgatggcggc
     9541 cagcgcggcc tggacatgct cgcgcgccac cagcacgaac acctcgtcgc cggccagcac
     9601 gcgggtgtcg gcctcgcagg gggtttcttc gtcgcgccgg tacaccgcca ccaggcgcat
     9661 cggcacctcg ggcacctggg cgatgaagtc ggcgatgctg cggccgtcaa gcgcgctgcc
     9721 gcgcgccacg cgcgccgcca ccaggtgcgc gcgcccctgc gagaactgca gcacctgcag
     9781 cgcctcgggg tgttcgatca gctcctggat ggtgcgcgtg acggacgcct cgggacagat
     9841 cacgcggctg acggcaaagc cttccggtcc cagcaggcga tcgtcctggc ggaagtcggg
     9901 caggcgcagc cgcgcgatgg tggtggggat gccgaacacg tcgtgcgcca ccttgcaggc
     9961 caccaggttg gtggcgtcca ggtcggcgca ggcgatgaac aggtcgcagt cgtcggcgcc
    10021 ggcgtggcgc agcaccgagg gctgaacgcc gttgccggcc acgccgcgca gctccagccg
    10081 ctcctccagc ccgcgcaggc gctcggggtc ggggtcgatg atggtgatgt cgttggcctc
    10141 cgaggccagc gtctcggcca cgctctgccc cacgcggccg gcgccaagaa tcaggatctt
    10201 catcgggggc gacctccgaa cgatcggcca atgctcatat ttttatagct gcttgcgctt
    10261 tcaggacggg cgccagaggg cggaaacacc taggacagat caccatccag gtaaaaccag
    10321 cgccagccgc cctggacatt ctcgcgcacg aaacggctgc gctcgtgcag ccgcgtggcg
    10381 cgcccgccgg cgacgcgcga gcgcgcgacg aactcgacct cggcgtgcgt ttcgtccagc
    10441 acccggtgcg cgcgcacctc caggcccagc catcggatgt gcggctccag cgccagcgtg
    10501 gccgggcgct ggctggcgtg ccagctggac agcaggtagt cggcgtcgcc gcgcacgaag
    10561 gcggtgtagc gcgagcgcat cagcgcctcg gcgtcgggcg c
//
LOCUS       DAOOOG010000085        10570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_7121_length_10570_cov_32.382977, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000085 DAOOOG010000000
VERSION     DAOOOG010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7121_length_10570_cov_32.382977"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PK242_07720"
     CDS             complement(<1..800)
                     /locus_tag="PK242_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOZ93942.1"
                     /translation="MPATEATAAPAAERALSAPQKALRKLGLVRPIDLALHLPLRYED
                     ETHLTPLAEAARTLSDAPLQFEATVVHHEVAYRPRRQLLVTVEDGGERCLLRFFNFYP
                     SQLKQMAVGTRLRVRGELRGGFGGLAMMHPVVRAAGGALPQALTPIYPTVAGLPQPYL
                     RKAVLGGLEQADLSDTLPDEVLSEIGHLRPWDLRKALSFLHHPTPDTALAQLEDHSHP
                     AWQRLKAEELLAQQLSQLRARRARQHLRAPVLPAQRGGLHEALLAALP"
     gene            854..1984
                     /gene="queA"
                     /locus_tag="PK242_07725"
     CDS             854..1984
                     /gene="queA"
                     /locus_tag="PK242_07725"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOZ93943.1"
                     /translation="MSDRTFTTSDFDFALPPELIAQHPAPERSASRLLDGSGAAPIDR
                     VFRDLPALLQPGDLLVFNDTQVVKARLYGHKPPAPLARSRRSPQGAPIGLGRPGADGQ
                     PSGGKLELLVERVLPDGSVAAHMKVSKKPPPGTVLRMAGGFTATVLGRWPQADGPLFH
                     LRLSGDAYQLMAAHGHVPLPPYIQHTDSAQDVARYQTVFARHPGAVAAPTAALHFDDA
                     LLAAIDARGVQRAFVTLHVGAGTFQPVRTENLAEHLMHRERYLVPPATAQAVAECRAR
                     GGRVVAVGTTSVRTLESWAASGQRQGDTDIFITPGFGFRVVDALVTNFHLPRSTLMML
                     VSALAGHDHVMALYAHAVAARYRFFSYGDAMWLTRRDGEPAR"
     gene            2273..3598
                     /locus_tag="PK242_07730"
     CDS             2273..3598
                     /locus_tag="PK242_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ93944.1"
                     /translation="MLASTPTADPFTLQLQQIRGLIEAHQLPQAAQALNAAQQQAPKD
                     ARIPLLGMRLAERAGNLERATQAARRALALAPDWHVAQIELALLLARQKQGDEAISLA
                     MRALASAPQEAQVMVGAINVALSAERHEQTLKWAEDGVRRFPESAGIRLFLARFLAAQ
                     GRLADATTHYEFILQRLPHHEETLQGLLSCALSMDDQARARTYAERLLALKPFDENAR
                     YWHAVVHGQTPPTQPAQVVTGIFDGYADQFDVHLVRGLQYRVPERVAQILLEHHPDRH
                     FNLLDLGCGTGLVGVYLGRIEGHIIGVDLSEKMIEQAARHQLYSRFHRVNVLDALRET
                     PAEHYEAITCTDVLVYVGDLAPVIPNALRILKPGGHFIFSCEAAAEDEADLVLRPSQR
                     YAHKASAVERQCREAGFDDIRIEHLPTLRMENDQPLPGFLVTARKPPAA"
     gene            3606..4778
                     /gene="tgt"
                     /locus_tag="PK242_07735"
     CDS             3606..4778
                     /gene="tgt"
                     /locus_tag="PK242_07735"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOZ93945.1"
                     /translation="MLRFELLGQDPSSHARRGRLTLNHGVVETPIFMPVGTYGTVKGV
                     LPRSLREMGAQIILGNTFHLWMRPGLDVLRQFGGLHGFENWHQPILTDSGGFQVWSLG
                     EMRKISEEGVKFASPVNGDRLFLTPEISMQIQTVLNSDIVMQFDECTPYETRGHVTTE
                     AEARASMALSLRWARRSQAEFERLGNPNALFGIVQGGMFEQLREESLAQLVELDLPGY
                     AIGGVSVGEPKEDMLRITAHTPHRLPAHKPRYLMGVGTPEDLVYGVQHGVDLFDCVMP
                     TRNARNGHLFTRFGDLKIRNARHKSDPRPIDESCTCHTCAGASGVAWQDGGREGFSRA
                     YLHHLERCGEMLAPMLASIHNLHYYLNLMRDIRAALDAGAFGAFRQRFAAERARGV"
     gene            4873..5847
                     /locus_tag="PK242_07740"
     CDS             4873..5847
                     /locus_tag="PK242_07740"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOZ93946.1"
                     /translation="MKTRITELFGIEHPIIQGGMHYVGFAELAAAVSNAGGLGVITGL
                     TQKTPEQLAREIARCRDMTDRPFGVNLTFLPTVSSPDYPGYIRAIIEGGVKAVETAGN
                     NPQQWLPLLKEHGVKVIHKCTSVRHALKAEAIGCDAVSVDGFECGGHPGEDDVPNFIL
                     LPRAAETLKIPFVASGGMADGRSLVAALALGAEGMNMGTRFIATQEAPVHEHVKQAIV
                     AASELDTRLVMRPLRNTERVLNNAAVERLLAKEKELGANLKFEDIIEQVAGVYPRIMQ
                     NGDMDAGAWSCGMVAGLIHDIPTVMALIDRIMAEAEALITQRLAGLVR"
     gene            complement(5921..6991)
                     /locus_tag="PK242_07745"
     CDS             complement(5921..6991)
                     /locus_tag="PK242_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase"
                     /protein_id="HOZ93947.1"
                     /translation="MSKALRLSEKWFQRGLWLVALVFTSFLIGLGSTLVGDLPRVESV
                     PALDDFMDRQAAQAARDSVQAGERQAQDHADALEQARLKLQVAQSDVGAQRQSFANWI
                     ATRRATERAEQDPELLARTRALDELHQRERAARAELERQQQALLDARQSVEQARRTLA
                     DMQRNAQGAYEQALRVQELRVFGYRLALTLPLLALAGWLFARQRQSRWWPFVWGFILF
                     AVFVFFVELVPYLPSYGGYVRYTVGIVATVLVGRWAIVALQRYLERQRAAEAQPDAQR
                     RAELGYDTALARLAKGVCPGCERTVDLKDGRTDFCPHCGIGLFDRCGPCQARKSAFAR
                     FCPACGSSAASPSPAAMALPHN"
     gene            complement(7069..7860)
                     /locus_tag="PK242_07750"
     CDS             complement(7069..7860)
                     /locus_tag="PK242_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOZ93948.1"
                     /translation="MFKLTSLNLNGIRSAATKGVQAWLEAHAPDCICVQEIKAQSPDL
                     AGRFESLAGLRGHFHLAEKKGYSGVGLYTRHKPSAVQAGFGSPEFDAEGRYLEARFDT
                     PRRRFSLISAYFPSGSSGPERQQAKFRFLAEFYPHLQRLKRERDFILCGDVNIAHQEI
                     DLKNWKSNQKNSGFLPEERAWMSQVLDELQLVDVYRRLHPDTTGDSYTWWSNRGQAYA
                     KNVGWRLDYHLATPALGACARAAAVYKAQKFSDHAPITIDYAFSL"
     gene            7874..8569
                     /gene="pyrE"
                     /locus_tag="PK242_07755"
     CDS             7874..8569
                     /gene="pyrE"
                     /locus_tag="PK242_07755"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOZ93949.1"
                     /translation="MSVTLPTRDEQNRLAQEFVQFAIESGVLRFGEFKTKAGRLSPYF
                     FNAGLFDDGAKLGRLAQFYARALLASGIEFDLIFGPAYKGIPLGAAVAIELARLGVNK
                     PFAYNRKEAKDHGEGGTLVGAPLAGRVLIVDDVISAGTAVRESIALIQAAGATPHAVA
                     IALDRQEKATENGQDVPWSAVQYVRETLGLQVCAIASLSDLLQHLSHTAHFSAHYDAV
                     SAYRARYGVTQTT"
     gene            8566..9279
                     /locus_tag="PK242_07760"
     CDS             8566..9279
                     /locus_tag="PK242_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOZ93950.1"
                     /translation="MNASPAAARSPAATGAAWRWRALAAGALLLGGGVGALAQGGGAA
                     PSSGIYTCIDAHGRRLTSDRPIAACIDREQRELNRDGTVRRVIPPTLSATEREALEAR
                     EREAALKRQRERDLLRRDQALLTRYPDQAAHDAGRAEELAQTQQVIQAAQQRIAELGQ
                     ERKALDDEMEFYVRDPARAPAKLRRALTDNAEAVAAQRRAIAAQQEEQRRIHARFDAE
                     ALRLQALWPTARAGDAPKR"
     gene            complement(9346..>10570)
                     /gene="gatB"
                     /locus_tag="PK242_07765"
     CDS             complement(9346..>10570)
                     /gene="gatB"
                     /locus_tag="PK242_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOZ93951.1"
                     /translation="RKNYFYPDLPKGYQISQFQQPVVQGGEVSFFVGPSTGSGQAQEK
                     KTVRLVRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVTEPDMRSSAEAVAYARE
                     LHKIVTWIGVCDGNMQEGSFRCDANVSVRKPGPPLGTRREIKNLNSFKFMQQAIDYEI
                     RWQIEQLEDGHAIQQATVLFDPDSGETRAMRTKEDSADYRYFPDPDLPPLCISESWIE
                     QERAQMPELPRQMAERLVAQHGLPEYDAATLTQSPAMAAYFEQAAAASGQPKLASNWV
                     MGELSRRLNAEDIGIEAARVSAAQLAQLIGRIQDGTISNAAARQVFEVLWRGEGETVD
                     AVIEAKGLRQMNDSAALEKIVDEVIAANPGNVEQFKAGKDKAFNALVGQVMKASRGKA
                     NPQQASELLRARLGA"
BASE COUNT         1661 a         3765 c         3557 g         1587 t
ORIGIN      
        1 aacggcagcg ccgccagcag cgcctcgtgc aggccaccgc gctgggcagg cagcacgggc
       61 gcgcgcaggt gctggcgcgc gcgccgcgcc cgcagctgcg acagttgctg cgccagcagc
      121 tcctcggcct tcaggcgctg ccaggcgggg tggctgtggt cttccagctg ggcgagcgcc
      181 gtatcgggcg tcgggtggtg caaaaaagat agcgctttgc gcaggtccca cgggcgcaga
      241 tggccgattt cgctcaaaac ctcgtcgggc agcgtgtcgc tcagatcggc ctgctccagc
      301 ccccccagca ccgccttgcg caggtagggc tgcggcagcc cggccaccgt ggggtaaatc
      361 ggcgtcagcg cctgcggcag cgcgccgccg gcggcgcgca ccaccggatg catcatggcg
      421 aggccaccga agccaccgcg cagctcgccg cgcacacgca ggcgcgtgcc caccgccatc
      481 tgcttgagct gcgagggata gaaattgaag aagcgcagca gacagcgctc gccaccgtct
      541 tccaccgtca ccagcagctg gcggcgcggg cgatacgcca cttcgtgatg caccacggtc
      601 gcttcaaact gcagcggcgc gtcgctcagg gtgcgcgccg cctcggccag cggcgtcagg
      661 tgggtttcgt cctcgtagcg cagcggcagg tgcagcgcca gatcgatcgg ccgcaccagg
      721 ccgagcttgc gcagcgcctt ctggggcgcg gacagcgcgc gctcggcggc gggggcggcg
      781 gtggcctcgg tggcgggcat gggacaattc tgccttccct tcttcttggt acgggcccgt
      841 ccggccctca agcatgtctg accgcacctt caccaccagc gatttcgact ttgccctgcc
      901 gcccgagctg atcgcccagc acccggcgcc cgagcgcagc gcctcgcgcc tgctggatgg
      961 cagcggcgcc gcacccatcg accgggtgtt tcgcgacctg ccggcgctgc tgcagccggg
     1021 cgacctgctg gtgttcaacg acacccaggt ggtcaaggcc cggctgtacg gccacaaacc
     1081 acccgccccg ttggcgcgtt cgcgccgttc ccctcaaggg gcgcccatcg gcttggggcg
     1141 gcccggcgcc gacgggcaac ccagtggcgg caagctggag ctgctggtcg agcgggtgct
     1201 gcccgacggc agcgtggccg cgcacatgaa ggtcagcaag aagcccccgc ccggcaccgt
     1261 gctgcgcatg gccggcggct tcacggccac cgtgctgggc cgctggccgc aggccgacgg
     1321 gccgctgttt cacctgcgtc tgtcgggcga tgcctaccag ctgatggcgg cgcacgggca
     1381 tgtgccgctg ccgccctaca tccagcacac cgactcggcg caggacgtgg cgcgctacca
     1441 gaccgtgttc gcgcgccacc ccggcgcggt ggcggcgccg acggcggcgc tgcacttcga
     1501 cgacgcgctg ctggccgcca tcgacgcgcg tggcgtgcag cgcgccttcg tcaccctgca
     1561 tgtgggcgcc ggcacctttc aaccggtcag gacggagaac ctggccgaac acctgatgca
     1621 ccgcgagcgc tacctggtgc cgcccgccac cgcgcaggcg gtggccgagt gccgcgcgcg
     1681 cggcggccgc gtggtggcgg taggcaccac cagcgtgcgc acgctggaga gctgggccgc
     1741 cagcggccag cggcagggcg acaccgacat cttcatcacc ccgggcttcg gcttccgtgt
     1801 ggtcgatgcc ctggtcacca acttccacct gccgcgcagc acgctgatga tgctggtcag
     1861 cgccctggcg ggacacgacc atgtgatggc gctgtacgcc cacgcggtgg cggcacgcta
     1921 ccgcttcttc agctatggcg acgccatgtg gctgacgcgc cgcgatggcg agcctgcccg
     1981 ctgaccgcag catcgtcgcg cttgacggac aagcgccggc gatcttcgtt gcagcctggc
     2041 aacggccatt ggacgcgccc gctcgtggcg cgcgccgatg cggtacggac aggtgcgtag
     2101 cgcgctcacc gaacaggtca aacccatcga tgccgcgaga ccccgttggg atgcacatct
     2161 caccaggggc accagcggtc aactggcgca ttgaggataa tccgggaacc gacatcaggg
     2221 ctaccgaccg cgcgctcgcg cggctctgac ggttcctccc ccaggtccac cgatgcttgc
     2281 ttccaccccc accgccgacc ccttcaccct ccagctgcag cagatccgcg gcctgatcga
     2341 agcccatcaa ctgccacagg ccgcgcaggc gctcaacgcg gcccagcagc aggcgcccaa
     2401 ggacgcccgc atcccgctgc tgggcatgcg gctggccgaa cgcgccggca acctggagcg
     2461 cgccacccag gcggcccgtc gcgcgctggc gctggcgccg gactggcatg tggcacagat
     2521 cgagctggcg ctgctgctgg ccaggcagaa gcagggcgac gaagccatct ccctggccat
     2581 gcgggcgctg gccagcgccc cccaggaggc tcaggtgatg gtgggtgcca tcaacgtggc
     2641 cctgtcggcg gaacgccatg agcagacact gaagtgggcc gaggacggcg tgcggcgctt
     2701 ccccgaaagc gccggcatcc gcctgtttct ggcgcgcttt ctggcggcgc aggggcggtt
     2761 ggccgacgcc accacccact acgagttcat cctccagcgc ctgccgcacc acgaggagac
     2821 cttgcagggc ctgctcagct gcgccctgag catggacgat caggcgcggg cccggactta
     2881 cgccgagcgt ctgctggccc tcaaaccctt cgacgagaac gcgcgctact ggcacgccgt
     2941 ggttcatggg caaacgccgc cgacgcagcc agcccaggtg gtgaccggca tcttcgacgg
     3001 ctacgccgac cagttcgacg tccatctggt gcgtggcctg cagtatcggg tgcccgagcg
     3061 cgtggctcaa atcctgttgg agcatcaccc ggaccgccac ttcaacctgc tcgacctggg
     3121 ctgcggcact gggctggtgg gtgtctacct gggccgcatc gaagggcaca tcatcggcgt
     3181 cgatctgtcg gaaaagatga tcgaacaagc cgcacgccac cagctctatt cgcgctttca
     3241 tcgcgtgaac gtgctcgacg ccctgcgcga aacgccggcc gagcactacg aagccatcac
     3301 ctgcaccgac gtgctggtct acgtgggcga tctggcgccg gtgattccca acgcgctgcg
     3361 catcctcaaa cccggcgggc atttcatctt ctcgtgcgag gccgccgccg aggacgaagc
     3421 cgacctggtg ctgcgtccca gccagcgcta cgcccacaag gccagcgcgg tcgagcgcca
     3481 gtgccgcgaa gccggcttcg acgacatccg catcgaacac ctgcccacgc tgcgcatgga
     3541 aaacgaccag ccgctgccgg gctttctggt gacggcgcgc aagccgccgg cggcctgacg
     3601 cagccatgct gcgcttcgaa ctgctgggcc aggacccgtc cagccacgcg cgccgcggac
     3661 gcctgacgct gaaccacggt gtcgtcgaga cgccgatctt catgcccgtc ggcacctacg
     3721 gcaccgtcaa gggggtgctg ccgcgcagcc tgcgcgagat gggcgcgcag atcatcctgg
     3781 gcaacacctt ccacctgtgg atgcggcccg ggctggacgt gctgcggcag ttcggcggtc
     3841 tgcacggctt cgagaactgg caccaaccca tcctgaccga ctcgggcggc tttcaggtct
     3901 ggtcgctggg cgagatgcgc aagatcagcg aggagggcgt gaaattcgcc agcccggtca
     3961 acggcgaccg gctgtttctc acgcccgaga tcagcatgca gatccagacc gtgctgaaca
     4021 gcgacatcgt catgcagttc gacgaatgta cgccctacga gacgcgcggc cacgtcacca
     4081 ccgaggccga agcgcgcgcc agcatggcgc tcagcctgcg ctgggcacgc cgcagccagg
     4141 ccgagttcga gcgcctgggc aaccccaacg cgctgttcgg catcgtacag ggcgggatgt
     4201 tcgagcagct gcgcgaagaa tcgctggcgc agctggtgga gctggacctg cccggctacg
     4261 ccatcggcgg cgtcagcgtg ggcgaaccca aggaagacat gctgcgcatc accgcccaca
     4321 cgccgcaccg cctgccggcg cacaagccgc gctacctgat gggcgtgggc acgccggaag
     4381 acctggtgta cggcgtgcag catggggtcg acctgttcga ctgcgtgatg cccacgcgca
     4441 acgcgcgcaa cggccacctg ttcacgcgct tcggcgacct gaagatccgc aacgcccgcc
     4501 acaagagcga cccgcgcccg atcgacgaga gctgcacctg ccacacctgc gccggcgcca
     4561 gcggggtggc ctggcaggac ggcgggcgcg agggcttttc gcgcgcctac ctgcaccatc
     4621 tggagcgctg cggcgagatg ctggcgccga tgctggcgtc catccacaac ctgcactact
     4681 acctgaacct gatgcgcgac attcgcgccg cgctcgatgc cggcgcgttc ggcgccttcc
     4741 gccagcgctt cgccgccgaa cgcgcgcgcg gcgtctgatg gccgtggcgc ccaggtgact
     4801 gcggcggcgc cacgccgcgc ctatgctgcg cggctctgcc ccctgacccc caccctttcc
     4861 ggagcctcca ccatgaagac ccgcatcacc gagctgttcg gcattgaaca ccccatcatc
     4921 cagggcggca tgcactacgt cggctttgcc gagctggccg ccgccgtctc caacgccggg
     4981 gggctgggcg tcatcaccgg cctgacgcag aagacgcccg agcagctggc gcgcgagatc
     5041 gcgcgctgcc gcgacatgac cgaccggccc ttcggcgtca acctcacctt tctgcccacc
     5101 gtcagctcgc ccgactaccc gggctacatc cgcgccatca tcgaaggcgg cgtgaaggcg
     5161 gtggagacgg cgggcaacaa cccgcagcag tggctgccgc tgctgaagga gcacggcgtc
     5221 aaggtgatcc acaaatgcac ctcggtgcgc cacgcgctga aggccgaggc catcggctgc
     5281 gacgccgtca gtgtcgacgg cttcgagtgc ggcggccacc cgggcgagga cgacgtgccc
     5341 aacttcatcc tgctgccgcg cgccgccgag acgctgaaga tccccttcgt cgcctcggga
     5401 ggcatggccg acgggcgcag cctggtggcg gcgctggccc tgggcgccga gggcatgaac
     5461 atgggcacgc gcttcatcgc cacccaggaa gcgccggtgc acgagcacgt caagcaggcc
     5521 atcgtcgccg ccagcgagct ggacacccgg ctggtgatgc gcccgctgcg caacaccgag
     5581 cgcgtgctga acaacgccgc cgtcgagcgc ctgctggcca aggaaaagga gctgggcgcc
     5641 aacctgaagt tcgaggacat catcgagcag gtggccggcg tctatccgcg catcatgcag
     5701 aacggcgaca tggacgccgg cgcctggagc tgcggcatgg tggccggcct gatccacgac
     5761 atcccgaccg tgatggcgct gatcgaccgc atcatggccg aggccgaggc gctgatcacg
     5821 cagcgcctgg cggggttggt gcgctgagcc cgtatccagc gcccaatcgc catccggcgc
     5881 ttgtccagca agcgccatta gctactttaa ttgtagcaat ctagttgtgc ggcagcgcca
     5941 tcgcggccgg cgatgggctg gcggcgctgc tgccacaggc ggggcagaag cgggcaaacg
     6001 cgctcttgcg cgcctggcag gggccacagc gatcgaacag accgatgccg cagtgcgggc
     6061 agaagtcggt cctgccgtcc ttcaggtcca ccgtgcgctc gcagcccggg cacacgccct
     6121 tggccaggcg cgccagcgcc gtgtcgtagc ccagttcggc gcggcgctgc gcgtccggtt
     6181 gggcctcggc cgcgcgctga cgctccaggt agcgctgcag cgccacgatg gcccagcgcc
     6241 ccaccagcac ggtggcgacg atgccgaccg tgtagcgcac atagccgccg tagctgggca
     6301 ggtagggcac cagctcgacg aagaacacga acaccgcaaa caggatgaag ccccacacga
     6361 agggccacca gcggctctgc cgctgacggg cgaacaacca gccggccagc gccagcagcg
     6421 gcagcgtcag cgccagccgg tagccgaaca cgcgcagctc ctgcacccgc agcgcctgtt
     6481 cgtaggcgcc ctgggcgttg cgctgcatgt ccgccagcgt gcggcgcgcc tgctcgaccg
     6541 actggcgcgc atccagcaac gcctgctgct gccgctccag ctcggcgcgg gcggcgcgct
     6601 cgcgctggtg cagttcgtcc agcgcacggg tgcgggccag cagctcgggg tcctgctcgg
     6661 cgcgctcggt ggcgcggcgg gtggcaatcc agttggcaaa gctctgccgc tgggcgccga
     6721 cgtcggactg cgccacctgc agcttcagcc gcgcctgctc cagcgcgtcg gcgtggtcct
     6781 gcgcctggcg ttcgccggcc tgcacgctgt cgcgcgcggc ctgggcggcc tgacggtcca
     6841 tgaagtcgtc cagcgccggc acgctctcga cccgcggcag atcgcccacc agggtgctgc
     6901 ccaggccgat caggaagctg gtaaacacca gggccaccag ccacagaccg cgctgaaacc
     6961 atttctccga caggcgcagg gctttgctca tctcacgtcc attctcaagc aataacggca
     7021 acggcgcgct accaacaagc gccggcagct atatttaaca tagcgccctc aaagggaaaa
     7081 cgcgtagtcg atggtgatgg gcgcgtggtc gctgaacttc tgcgccttgt acaccgccgc
     7141 cgctcgcgca cacgcaccga gcgccggcgt ggccaggtgg tagtccagcc gccagcccac
     7201 gttcttggca taagcctggc cgcggttgct ccaccaggtg taggaatcgc cggtggtgtc
     7261 ggggtgcagg cggcggtaga cgtccaccag ttgcagttca tcgagcacct ggctcatcca
     7321 ggcgcgctcc tcgggcagaa aaccgctgtt cttctggttg ctcttccagt ttttgaggtc
     7381 gatttcctgg tgcgcgatgt tgacgtcgcc gcacaggatg aagtcgcgct cgcgcttgag
     7441 gcgctgcagg tgcgggtaga actcggccag aaagcggaac ttggcctgct gccgctcggg
     7501 gccggaggag ccactgggaa agtaggcgct gatgagcgaa aaccggcgcc gcggggtgtc
     7561 gaaacgcgcc tccagataac ggccctcggc gtcgaactcg ggcgagccga agccggcctg
     7621 cacggcgctc ggtttatggc gggtgtagag gccgacgccc gaatagcctt tcttctcggc
     7681 cagatgaaag tgcccgcgca gcccggccag cgactcgaag cggccggcca gatcgggcga
     7741 ctgggccttg atctcctgca cacaaataca atccggcgcg tgcgcctcca gccaggcctg
     7801 cacgcccttg gtggcggccg agcggatgcc gttgaggttg aggctggtga gcttgaacaa
     7861 ggaacggacc cccatgagcg tgacactgcc aacccgtgac gagcaaaacc ggctggcgca
     7921 ggaattcgtg caattcgcca tcgaaagcgg cgtgctgcgc tttggcgaat tcaagaccaa
     7981 ggcagggcgc ctgtcgccct atttcttcaa tgccggcctg ttcgacgacg gcgccaagct
     8041 gggccggctg gcgcaattct atgcgcgcgc cctgctggcg tcgggcatcg agttcgacct
     8101 catcttcggc ccggcctaca agggcattcc gctgggcgcg gcggtcgcca tcgagctggc
     8161 gcgcctgggc gtcaacaagc cgtttgccta caaccgcaag gaagccaagg accacggcga
     8221 aggcggcacc ctggtcggcg cgccgctggc cggtcgggtg ctgatcgtcg acgacgtgat
     8281 ctccgccggc accgcggtgc gcgaatcgat tgccctcatc caggccgccg gcgccacgcc
     8341 gcacgcggtg gcgatcgcgc tggatcgtca ggaaaaggcc accgaaaacg gccaggacgt
     8401 gccctggtcg gccgtgcagt acgtgcgcga aacgctgggg ctgcaggtct gcgccatcgc
     8461 cagcctgtcg gatcttctgc aacacttgtc acatactgca cacttctcag cgcattacga
     8521 cgccgtgtcg gcctatcggg cacggtacgg cgttacacag acgacatgaa cgcatcgcca
     8581 gcagctgccc gctcgcctgc cgccaccggc gcggcatggc ggtggcgtgc gctggcggcc
     8641 ggtgcgctgc tgctgggggg tggcgttggc gcgctggccc agggtggtgg cgcggcgcca
     8701 tccagtggca tctacacctg catcgacgcc catggccggc gcctgacgtc ggaccgccca
     8761 atcgcggcct gcatcgaccg cgagcagcgc gagctcaacc gcgacggcac cgtgcggcgc
     8821 gtcatcccac ccacgctcag cgccaccgag cgcgaggccc tcgaagcgcg cgagcgcgaa
     8881 gcggcgctca agcgccagcg cgaacgcgat ctgctgcgcc gcgatcaggc gctgctcacc
     8941 cgctaccccg accaggccgc gcacgacgcc gggcgcgccg aagagctggc gcagacccag
     9001 caggtgatcc aggcggcgca gcagcgcatc gccgagttgg gccaggagcg caaggcgctg
     9061 gacgacgaga tggagttcta cgtccgcgac ccggcccgcg ccccggccaa gctgcgccgc
     9121 gcgctgaccg acaacgccga ggcggtggcg gcccagcgcc gcgccatcgc cgcccagcag
     9181 gaagagcagc gccgcatcca cgcccgcttc gacgccgagg cgctgcgcct gcaggcgctg
     9241 tggccgaccg cccgcgcggg cgatgcgccc aagcgctgat gcaaccaaat caagcgctgg
     9301 cgcctgtcca gactgcgtca gcagctatta taaatatagc tttcgtcaag cacccaggcg
     9361 ggcccgcagc agctcgctgg cctgctgtgg gttggccttg ccgcggctgg ctttcatcac
     9421 ctggccgacc agggcgttga aggccttgtc cttgccggcc ttgaactgct cgacgttgcc
     9481 ggggttggcg gcgatcacct cgtcgacgat cttctccagc gccgcgctgt cgttcatctg
     9541 cctgaggccc ttggcctcga tcacggcgtc caccgtctcg ccctcgccgc gccacagcac
     9601 ttcgaacacc tggcgcgcgg cggcgttgct gatggtgccg tcctggatgc ggccgatcag
     9661 ctgcgccagc tgcgccgccg acacgcgcgc cgcttcgatg ccgatgtcct cggcgttcag
     9721 gcgccgcgaa agttcgccca tcacccagtt gctggccagc ttgggctgac cgctggcggc
     9781 cgccgcctgc tcgaagtagg cggccatggc gggcgactgc gtcagggtcg cggcgtcgta
     9841 ttcgggcagg ccatgctggg ccaccaggcg ctccgccatc tggcgcggca gctcgggcat
     9901 ctgggcgcgc tcctgttcga tccatgactc cgaaatgcac agcggcggca ggtcagggtc
     9961 ggggaagtag cggtagtcgg ccgagtcttc cttggtgcgc atggcgcgcg tctcgccgct
    10021 gtcagggtcg aacagcacgg tggcctgctg gatcgcgtgg ccgtcttcca gctgctcgat
    10081 ctgccagcgg atctcgtagt cgatcgcctg ctgcatgaac ttgaagctgt tcaggttctt
    10141 gatctcgcgc cgcgtgccca gcggcgggcc gggcttgcgc acgctgacgt tggcgtcgca
    10201 gcggaagctg ccttcctgca tgttgccgtc gcacacgccg atccaggtga cgatcttgtg
    10261 cagctcgcgc gcgtaggcca cggcctcggc gctgctgcgc atgtcgggct cggtgacgat
    10321 ctccagcagc ggcgtgccgg cgcggttcag atcgatgccg ctcatgccgt gaaagtcctc
    10381 gtgcagcgac ttgccggcgt cttcttccag gtgggcgcgc accaggcgca cggtcttttt
    10441 ctcttgagcc tgtcctgagc ctgtcgaagg gccgacgaag aacgacactt cgccgccctg
    10501 caccaccggc tgctggaact ggctgatctg gtagcccttg ggcaggtcgg ggtagaagta
    10561 gttcttgcgg
//
LOCUS       DAOOOG010000086        10506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_7178_length_10506_cov_33.054827, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000086 DAOOOG010000000
VERSION     DAOOOG010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10506
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7178_length_10506_cov_33.054827"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..781
                     /gene="metW"
                     /locus_tag="PK242_07770"
     CDS             197..781
                     /gene="metW"
                     /locus_tag="PK242_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOZ93952.1"
                     /translation="MSDEPIRRVIAGLVPEGAHVLDLGCGDGALLDHLLRQRRCTGYG
                     IEIDDANVLACVQRGVNVLQLNLHQGLAAFQDQSFDVVLQIDTLQHLRNAEVMLRETA
                     RVGRIGIIAFPNFAHWPNRLSVLRGRMPVTRRLPYQWYDTPNIRVGTFKDFEALARKN
                     GLQLLDAFGLEEGRVVRWLPNARASTAVFKLARR"
     gene            complement(789..2516)
                     /locus_tag="PK242_07775"
     CDS             complement(789..2516)
                     /locus_tag="PK242_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOZ93953.1"
                     /translation="MAKVLIANRGEVAVRIARACRDYGVRSVAVYADPDADALHVRAA
                     DEAWALPGTRPADTYLAIDRLLAVARQSGADAVHPGYGFLSERAEFARAVLAAGLTWI
                     GPRPETIEALGDKVAARRIALQVGAPLVAGTAEPVGSADEVLAFARRHGLPLAIKAAY
                     GGGGRGLKVAWQMDEVVELYASAVREATAAFGRGECFLEQFLDRPRHIEAQVIADRHG
                     HVVVLGTRDCSLQRRHQKLVEEAPAPFLSDAQRAQVHQAARDICAAAGYESAGTVEFL
                     LGQSGQISFLEVNTRLQVEHPVTEETSGVDLVLEQLRVADGLPLSFTQAPPVRGHSFE
                     FRLNAEDPGRGYLPTPGRITRFDLPAGPGVRVDTGFAAGSTVPGSFDSLLAKLIVTGA
                     TRAQALARARRALAECRIEGVASVLPFHRAVVDDPAFVGADGLQVHTRWIETEQQASF
                     EPAPRADDPDHAPLLRSFIEVDGRRLSLGLPAGLALGVGAAAAPAAPPSAAPTAEAGD
                     VLAPLTGSLVRWHRQTGEPVAAGEAIVTLEAMKMETVVSAPCAGTLECLAAPGDMLTA
                     GQRLARVRG"
     gene            complement(2516..4174)
                     /locus_tag="PK242_07780"
     CDS             complement(2516..4174)
                     /locus_tag="PK242_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HOZ93954.1"
                     /translation="MRLLPASDTALLVELADLEQTLALYRAAQAQPIDGVQELVPAAR
                     TLLVHYRPEAVTPTELAAALRARQAAAQAGGQPVAGTRAARQVEIVVRYDGEDLPAVA
                     ERLGLSVADVIARHTAQPWQAAFAGFAPGFVYLAGGHASLRGIPRRPSPRTRVPAGAV
                     ALAGDFSAVYPAASPGGWQLIGVTAVPMWDLRRAEPAYVQPGFQVQFIDQRTAGRHIS
                     LPAVPDSESNKPLAPDQQAPEAIEFEAVGLQTLVQDAGRHGMAGLGISPSGALDLPAM
                     RQANRLVGNPVHTPVLEHLLGGLRLRCHGRATLAVSGAVAPLTLHAPDGRQWPVPGHA
                     ALALDDGDVLAIGAPCAGVRCYVAVRGGWQVAPVLGSCATDTLAHVGPPALQAGQRLA
                     VGGAVPAQALRATQDAAAPAPELPRAGAQVTLDVVAGPRTDWFGPEALARLTQQPWQV
                     TPQSNRIGLRLHGAEPLARARHDELPSEGTVTGAIQVPAGGQPVLFLADHPLTGGYPV
                     IAAVARHHLPLAAQLAAGCQLRFRLVAPFAELGDAHDTLPEKPA"
     gene            complement(4178..4936)
                     /locus_tag="PK242_07785"
     CDS             complement(4178..4936)
                     /locus_tag="PK242_07785"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOZ93955.1"
                     /translation="MDINSDLGESYGAWRMGDDAAMLDIVTSANVACGFHAGDPAGIL
                     HTLRAAAARGVAVGAHVAYPDLVGFGRRAMDLGSDELVADVIYQIGALQGLAQAAGTR
                     VRYVKPHGALYNTIAHDARQGDDVISALLALDRKLVLLALAGSPLIGRARARGLTVVA
                     EAFADRAYNDDGTLVSRRLPGAVLHDADQVAERMLRWSESGTLTSISGRPVRLEAESI
                     CVHGDSPGAVAMARAVRARLSAAGVPLGAFIAAD"
     gene            5062..6420
                     /locus_tag="PK242_07790"
     CDS             5062..6420
                     /locus_tag="PK242_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOZ93956.1"
                     /translation="MTDLPTIDQLSAELAAGRRSAVQLTEAALARAQDPAGEGARVFT
                     RLDPARALAAARASDQLRAAGIVRSPLEGIPVSIKDLFDVAGQVTTAGSAVLRDAPPA
                     ARSAPVVERLIAAGAVIVGRTNMTEFAFSGLGLNPHYGTPANPWQRAERRIPGGSSSG
                     AAVSVSDGMAAVAIGSDTGGSVRIPSALCGLTGFKPTQRRVPTAGALPLSTSLDSIGP
                     LGASVRCCALTDAVLAGQSLLPLPARPLRDARLALPDTLVFDDLEPAVAAAFERACAR
                     LQAGGARLQRIAVPEFAELGRLHARGTLAAAEAWAWHRQLLATRAAEYDPRVGLRLQG
                     GAQMSAADYLDLQAARRRWIEQVEARLAPFDALLLPTVPLLAPRIDALQASDADYFAA
                     NARMLRNPTLINFLDGCALSLPCHVPGEAPVGLMLAAAGGQDRALLALGEAVEQALRP
                     AP"
     gene            6627..7334
                     /locus_tag="PK242_07795"
     CDS             6627..7334
                     /locus_tag="PK242_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ93957.1"
                     /translation="MSTHRLLMIEDDARLAAMVGEYLTQSGYAVTHRGDGETGLQTLQ
                     GEPFDLVVLDLMLPGIDGLEVCRRIRALPDGASRVPVLMLTAKGDPMDRIIGLELGAD
                     DYLPKPFEPRELLARIRAVLRRQQPGAEPEHRLLRFGTLEIDRDARTVSVGGQPAELT
                     SYQFDLLVTLAERAGRVLTRDQIMEAVRGRELEAFDRSIDVHMGRIRAAIEQDVKNPR
                     RILTVRGVGYVFARQQD"
     gene            7420..8697
                     /locus_tag="PK242_07800"
     CDS             7420..8697
                     /locus_tag="PK242_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ93958.1"
                     /translation="MKVTDSIARRLYLRIWLAVAGSVLVLSLLIGWGWHAAEQERERD
                     RQAGQPREVLVRNAAGELIGTAEAQPRRLPGQGFEFVVTLRDGQVLSLQMPPRGRGRA
                     AAGAAARRHADLPPSWLRPPYGFWWLLALAGLGVMLGVYPVARRLTQRLEALQRGVQR
                     WGEGDLSARLPESGQDEVADLSRRFNAAAERIQTLMDAQGALLQSQKSLLANASHELR
                     SPLARIRMAMALAGPQGSPAARAEIERSITELDQLVDEILLASRLDAREADLGTVEPV
                     DLIGLLAEECARVGAEFTLADGTRQVVVPGVARLLRRAVRNLLENAVRHGRRTGRDGE
                     VGAHLQLAGGAAELQVDDRGPGVPPEQRERIFEPFYRLPGASEREGGVGLGLALVRSI
                     AQRHGGSVTCDHRPGGGARFTLRLPVEADAATK"
     gene            8940..10172
                     /locus_tag="PK242_07805"
     CDS             8940..10172
                     /locus_tag="PK242_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOZ93959.1"
                     /translation="MPQLPMVRWVALFWGFVAFFPIGLNYTAFVLLAVAMVWQGDRAE
                     RLARLRQAPQWWPAWALVGWSTLILLLGPHYAETPSNALHALRIVLTLALALALTRDE
                     ALWALRGVLLGLAVGLLLVALHGLVPLPPHPLWDSLVRYDGNKSLANAVQMAMVAAGS
                     LLLLPALGRRGQVLAALLAAAVMLVLIWVLHSRTAWLVLVLGLALGLWHRLRGRRRPQ
                     LAALATAALLTLAAGLLLPGVKQRLQQGAGELVSAYQGQTVEMDSSWGIRYRMYRETT
                     AMIAERPLLGWGIGGWNTQWRQRVNGPLTDVNMPHNDLLWMGAQTGVPGALLLLWLLL
                     AGWPAAWRRDDLNGSLAALALLAMLLAVLGNSALRDASMGLSLWFVVLVIQRLAHQPQ
                     PLWQGLWPAGAVAPQAGR"
     gene            complement(10217..10426)
                     /locus_tag="PK242_07810"
     CDS             complement(10217..10426)
                     /locus_tag="PK242_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HOZ93960.1"
                     /translation="MSTPNQVVELLARDLTRSGETFCPHPKADMKLWNTHPRVFLNLA
                     QGEARCPYCGTLYRMKAGEVVGAGH"
BASE COUNT         1331 a         3812 c         3923 g         1440 t
ORIGIN      
        1 gataccgaat gagcgcgccg ggccgctccc aagcgcgaat cccgcagcgc gtcagagcgg
       61 agggataccg aatgagcgcg ccgggccgct cccaagcgcg aatcccgcag cgcgtcagcg
      121 cggagggcac tccgctgagc gcgccgggcc gctcccaagc gcgaatcccg cagcgtgcag
      181 cgcggaggga tgttccatga gcgacgaacc gatccgccgc gtcatcgccg gtctggtgcc
      241 cgagggtgcg cacgtgctcg acctgggctg cggcgatggc gcgctgctcg accatctgct
      301 gcgccagcgc cgctgcaccg gctacggcat cgagatcgac gacgccaacg tgctggcctg
      361 cgtgcagcgc ggcgtcaacg tgctgcagct caacctgcac cagggcctgg ccgccttcca
      421 ggaccagagc ttcgacgtgg tgctgcagat cgacaccctg cagcacctgc gcaacgccga
      481 ggtcatgctg cgcgaaaccg cgcgcgtggg gcgcatcggc atcatcgcct tccccaactt
      541 cgcccactgg cccaaccgcc tgagcgtgct gcgcgggcgc atgccggtga cgcgccggct
      601 gccctaccag tggtacgaca cgcccaacat ccgcgtcggc acgttcaagg acttcgaggc
      661 gctggcgcgc aagaacggcc tgcagctgct ggacgccttt ggcctggagg agggacgggt
      721 ggtgcgctgg ctgcccaacg cccgcgccag cacggcggtg ttcaagctgg cgcggcgctg
      781 acggccgctc agccgcgcac gcgcgccagg cgctgaccgg cggtcagcat gtcgccgggc
      841 gcggccaggc attccagggt gccggcgcag ggtgcgctga ccacggtttc catcttcatg
      901 gcttccagcg tgacgatggc ttcgccggcc gccaccggct cgcccgtctg gcggtgccag
      961 cgcaccaggc tgccggtgag cggcgccagc acatcgcccg cctcggccgt cggggcggcg
     1021 ctcggggggg cggcgggggc agcggcagcg ccgacgccca gcgccagacc agcgggtagg
     1081 cccaggctca ggcgccggcc gtccacttcg atgaagctgc gcagcagcgg ggcgtgatcg
     1141 gggtcgtcgg cgcgcggtgc cggctcgaag ctggcctgct gctcggtttc gatccagcgc
     1201 gtgtgcacct gcaggccgtc ggcgccgacg aacgccgggt cgtcgaccac cgcgcgatgg
     1261 aacggcagca ccgaggccac gccctcgatc cggcattcgg ccagcgcccg gcgcgcgcgc
     1321 gccagcgcct gcgcgcgcgt ggcgccggtg acgatcagct tggccagcag cgagtcgaag
     1381 ctgcccggca ccgtgctgcc ggcggcaaag ccggtatcga cccgcacgcc ggggccggcc
     1441 ggcaggtcga agcgcgtgat gcgcccgggc gtgggcaggt agccgcggcc cgggtcctcg
     1501 gcgttgaggc ggaattcgaa gctgtggccg cgcaccggcg gcgcctgggt gaacgacagc
     1561 ggcaggccgt cggccacgcg cagctgctcg agcaccagat cgacgccgct ggtttcttcg
     1621 gtcaccgggt gctcgacctg caggcgggtg ttgacttcga ggaacgagat ctggccgctc
     1681 tgcccgagca ggaattccac cgtgccggcg ctttcgtagc cggcggcggc gcagatgtcg
     1741 cgcgccgcct gatgcacctg ggcgcgctgg gcgtcgctca gaaacggcgc cggcgcttct
     1801 tccaccagct tctggtggcg ccgctgcaac gagcagtcgc gggtgccgag caccaccacg
     1861 tggccgtgcc ggtcggcgat gacctgggcc tcgatgtggc gcgggcgatc cagaaactgc
     1921 tccagaaagc actcgccgcg cccgaacgcc gccgtcgctt cgcgcaccgc ggatgcgtac
     1981 agctcgacca cttcgtccat ctgccaggcc accttcaggc cccggccgcc gccgccgtag
     2041 gccgccttga tcgccagcgg caggccgtgc cggcgggcaa aggccagcac ctcgtcggcg
     2101 ctgcccaccg gctcggcggt gccggccacc agcggcgcgc ccacctgcag cgcgatgcgg
     2161 cgcgccgcca ccttgtcgcc cagcgcctcg atggtttccg ggcgtggccc gatccaggtc
     2221 aggccggccg ccagcaccgc gcgcgcaaac tcggcccgct cggacagaaa accgtagccc
     2281 gggtgcacgg cgtcggcgcc ggactggcgg gccacggcca gcagccggtc gatggccagg
     2341 taggtgtcgg ccgggcgtgt gcccggcagc gcccaggctt catcggcggc gcgcacgtgc
     2401 agggcgtcgg cgtcggggtc ggcgtacacc gccaccgagc gcacgccgta gtcgcggcag
     2461 gcgcgcgcga tgcgcacggc aacttcgccg cggttggcga tcaggacctt ggcaatcatg
     2521 cgggcttctc cggcagggtg tcgtgggcat cgcccagttc ggcaaacggg gcgacgaggc
     2581 gaaagcgcag ctggcagccg gcggcaagct gggccgccag cggcaggtgg tggcgggcga
     2641 cggcggcgat caccgggtag ccgccggtca gcgggtggtc ggccaggaac agcaccggct
     2701 ggccaccggc cggcacctgg atggcgccgg tgaccgtgcc ttcgctgggc agctcgtcgt
     2761 ggcgcgcgcg cgccagcggc tcggcgccgt gcaggcgcag gccgatgcgg ttggactgcg
     2821 gcgtgacctg ccacggctgc tgcgtcagcc gcgccagcgc ctcggggccg aaccagtcgg
     2881 tgcgcgggcc ggccaccacg tccagcgtga cctgcgcgcc cgcgcgcggc agctcgggcg
     2941 ccggcgctgc ggcgtcctgg gtggcgcgca gcgcctgcgc cggcaccgcc ccaccgacgg
     3001 ccaggcgctg gccggcctgc agcgccggcg ggccgacatg ggccagggtg tcggtggcgc
     3061 agctgcccag caccggcgcc acctgccagc caccgcgcac cgccacgtag cagcgcacgc
     3121 cggcgcaggg tgcgccgatg gccagcacgt cgccgtcgtc cagcgccagc gcggcatggc
     3181 cgggcaccgg ccactggcgg ccgtccggcg cgtgcagggt cagcggcgcc acggcgccgc
     3241 tgaccgccag cgtggcgcgg ccgtggcagc gcaggcgcag gccgcccagc agatgctcca
     3301 gcaccggcgt gtgcaccggg ttgccgacca gccggttggc ctggcgcatg gcgggcagat
     3361 ccagcgcgcc cgagggcgat atgcccagcc cggccatgcc gtggcgaccc gcgtcctgca
     3421 ccagagtctg caggccgacc gcttcaaatt cgatagcttc tggcgcttgc tggtcgggcg
     3481 ccagaggctt gtttgattcg gaatccggca cggccggcag gctgatgtgg cggccggccg
     3541 tgcgctggtc gatgaactgc acctgaaagc ctggctgcac ataggcgggt tcggcgcgcc
     3601 gcaggtccca catcggcacc gcggtgacgc cgatcagctg ccagccgccg ggcgaggcgg
     3661 ccgggtagac ggcgctgaag tcgcccgcca gcgccaccgc cccggccggc acgcgggtgc
     3721 gcgggctggg ccggcgcggg atgccgcgca ggctggcgtg gccgccggcc aggtaaacga
     3781 agccgggcgc gaacccggcg aacgccgcct gccagggctg ggccgtgtgg cgggcgatca
     3841 cgtccgccac cgacaggccc agccgttcgg ccacggcggg caggtcttcg ccgtcgtagc
     3901 gcaccacgat ctcgacctgg cgggcggcgc gggtgccggc caccggctgc ccgccggcct
     3961 gggccgcggc ctgccgcgcg cgcagcgccg ccgccagctc ggtcggcgtc accgcctcgg
     4021 gccggtaatg caccagcagc gtgcgcgcgg ccggcaccag ctcctgcaca ccgtcgatcg
     4081 gctgggcctg ggcggcgcgg tacagcgcca gggtctgctc gaggtcggcc agttcgacca
     4141 gcagcgcggt gtcgctggcg ggcagcaagc gcatggctca gtccgcggcg atgaaggcgc
     4201 ccagcggcac gccggcggcg ctcagccgcg cgcggacggc gcgtgccatc gccaccgcgc
     4261 cggggctgtc gccgtgcacg cagatcgact cggcttcgag gcgcaccggc cggccgctga
     4321 tcgacgtcag cgtgccgctt tcgctccagc gcagcatgcg ctcggccacc tggtcggcgt
     4381 cgtgcagcac ggcgccgggc aggcgtcgcg acaccagggt gccgtcgtcg ttgtaggcgc
     4441 ggtcggcaaa ggcctcggcc accacggtca ggccgcgcgc gcgggcgcgc ccgatcagcg
     4501 gcgagccggc cagcgccagc agcaccagct tccggtcgag cgccagcagg gcgctgatca
     4561 cgtcgtcacc ctggcgcgcg tcgtgggcga tggtgttgta gagcgcgccg tgcggcttga
     4621 cgtagcgcac ccgcgtgccg gcggcctgcg ccaggccctg cagggcgccg atctggtaga
     4681 tcacgtcggc caccagctcg tcgctgccga ggtccatggc gcggcggcca aagcccacca
     4741 ggtcggggta ggccacgtgc gcgcccaccg ccacgccgcg cgcggcggcg gcgcgcagcg
     4801 tgtgcaggat gcccgccggg tcgcccgcgt ggaacccgca ggccacgttg gcgctggtca
     4861 cgatgtccag catggcggcg tcgtcgccca tgcgccaggc gccgtagctc tcaccgaggt
     4921 cgctgttgat gtccatgacc gcatctcctg aaggggccgt ccgccgtgcg cgcgggccga
     4981 ccgcatgctc gccattctgg cgcagcggcg gcgcgggctt accatgccgc tcggttgccc
     5041 tcttcatcca gcgtttcgac catgaccgat ctgcccacca tcgaccagct gtcggccgaa
     5101 ctggccgccg gccgccgcag cgccgtgcag ctcaccgagg ccgcgctggc gcgcgcacag
     5161 gacccggcgg gcgaaggcgc gcgggtgttc acccggctcg acccggcgcg cgcgctggcc
     5221 gccgcgcgcg cgtccgatca gctgcgcgcg gccggcatcg tgcgctcgcc gctggagggc
     5281 attccggtgt cgatcaagga cctgttcgac gttgccgggc aggtcaccac cgccggctcg
     5341 gcggtgctgc gcgacgcgcc gccggcggcg cgcagcgccc cggtggtcga gcgcctgatc
     5401 gccgccggcg cggtgatcgt gggccgcacc aacatgaccg aattcgcctt ttccggcctg
     5461 ggcctgaacc cgcactacgg cacgccggcc aacccgtggc agcgggccga gcgacgcatt
     5521 ccgggcggct ccagcagcgg cgcggcggtg tcggtgagcg acggcatggc ggcggtggcc
     5581 atcggctccg acaccggcgg ctcggtgcgc atcccgtcgg ccctgtgcgg cctgaccggc
     5641 ttcaagccga cccagcggcg cgtgcccacg gcgggcgcgc tgccgctgtc gacgtcgctc
     5701 gactccatcg ggccgctggg cgccagcgtg cgctgctgcg ccctcaccga cgccgtgctg
     5761 gccggccagt cgctgctgcc gctgccggcc cgcccactgc gcgacgcgcg cctggcgctg
     5821 cccgacaccc tggtgttcga cgacctggag ccggcggtgg cggccgcctt cgagcgcgcc
     5881 tgcgcccgtc tgcaggctgg cggcgcccgc ctgcagcgca tcgccgtgcc cgaattcgcc
     5941 gagctcggcc gcctgcatgc gcgcggcacc ctggcggcgg ccgaggcctg ggcctggcac
     6001 cgccagctgc tggcgacccg cgcggccgaa tacgacccgc gcgtgggcct gcgcctgcag
     6061 ggcggggcgc agatgagcgc ggccgactac ctggatctgc aggcggcgcg gcgccgctgg
     6121 atcgagcagg tcgaagcgcg cctggccccg ttcgacgccc tgctgctgcc caccgtgccg
     6181 ctgctggcgc cgcgcatcga cgcgctgcag gccagcgacg ccgactactt cgccgccaac
     6241 gcgcggatgc tgcgcaaccc gacgctgatc aactttctgg acggctgcgc gctgtcgctg
     6301 ccctgccacg tgcccggcga ggcgccggtg ggcctgatgc tggccgccgc cggcgggcag
     6361 gaccgcgcgc tgctggcgct gggcgaggcg gtcgagcagg ccctgcgccc ggcgccctga
     6421 gccgaaccat cgaatgcggc atcgaatcgg cgtccagcgc tttggtggca agcgtgagca
     6481 gctatcgttt gaatagtcag ccgggcgccg ccgcaggccc ggtttacgat tctttaccgc
     6541 cccgacaagc ggcgctggca tggcgccgcg atcatggcgg gcagcatcgg gtggcgcgtg
     6601 cgccgcgccg agcgtgaaag gagcgcatga gtacccaccg cctgctgatg atcgaggacg
     6661 acgcccgcct ggccgccatg gtgggcgagt acctgacgca atcgggctac gccgtcacgc
     6721 accgcggcga cggtgaaacc ggactgcaga ccctgcaggg cgagccgttc gacctggtgg
     6781 tgctggacct gatgctgccc ggcatcgatg gcctggaggt ctgccggcgc atccgcgccc
     6841 tgcccgacgg ggcgtcgcgc gtgccggtgc tgatgctgac cgccaagggc gacccgatgg
     6901 accgcatcat cggcctggag ctgggcgccg acgactacct gcccaagccg ttcgagccgc
     6961 gcgagctgct ggcccgcatc cgcgccgtgc tgcggcgcca gcagccgggc gccgagcccg
     7021 aacaccgtct gctgcgcttc ggcacgctgg agatcgaccg cgacgcccgc accgtcagcg
     7081 tcggcggcca gccggccgaa ctcaccagct accagttcga cctgctggtg accctggccg
     7141 agcgcgccgg ccgcgtgctg acgcgcgatc agatcatgga ggcggtgcgc gggcgcgagc
     7201 tggaggcgtt cgaccgctcc atcgacgtgc acatgggccg catccgcgcc gccatcgagc
     7261 aggacgtgaa gaacccgcgc cgcatcctca ccgtgcgcgg cgtcggctac gtgttcgccc
     7321 gccagcagga ctgagcgtgc cgtccgccat ggcgatccaa tcggcctcca gcgcttgtca
     7381 ggcaagcgcc ggcagctaca tatttcatag ttcgtcggcg tgaaagtgac cgattccatt
     7441 gcccgcaggc tgtacctgcg catctggctg gcggtggccg gcagcgtgct ggtgctcagc
     7501 ctgctgatcg gctggggctg gcacgccgcc gagcaggagc gcgagcgcga ccgccaggcc
     7561 ggccagccgc gcgaagtgct ggtgcgcaac gccgcgggcg aactgatcgg caccgccgag
     7621 gcgcagccgc gccgactgcc cgggcagggc ttcgaattcg tcgtcaccct gcgcgacggc
     7681 caggtgctga gcctgcagat gccgccgcgc ggccgcggcc gcgctgccgc gggcgccgcc
     7741 gcccgccgcc atgccgacct gccgccgtcc tggctgcgcc cgccctacgg cttctggtgg
     7801 ctgctggcgc tggccggtct gggcgtgatg ctgggggtgt acccggtggc gcgccgcctg
     7861 acccagcggc tggaggcgct gcagcgcggc gtgcagcgct ggggcgaggg cgacctgtcg
     7921 gcgcgcctgc ccgaaagcgg ccaggacgag gtggccgacc tgtcgcgccg cttcaacgcc
     7981 gccgccgagc gcatccagac cctgatggac gcccagggcg cgctgctgca gtcgcagaag
     8041 tcgctgctgg ccaacgcctc gcacgagctg cgctcgccgc tggcgcgcat ccgcatggcc
     8101 atggcgctgg ccggcccgca gggttcgccg gccgcgcgcg ccgaaatcga gcgcagcatc
     8161 accgagctgg accagctggt cgatgaaatc ctgctggcca gccggctgga cgcgcgtgag
     8221 gccgacctgg gcacggtcga gccggtcgac ctgatcggcc tgctggccga ggaatgcgcg
     8281 cgcgtcggcg ccgaattcac cctggccgat ggcacccggc aggtggtggt gcccggcgtc
     8341 gccaggctgc tgcgccgcgc ggtccgcaac ctgctcgaaa acgccgtgcg ccatggccgc
     8401 cgcaccgggc gagacggcga agtcggtgct cacctgcaac tggctggtgg cgcggccgaa
     8461 ctgcaggtcg acgatcgcgg ccccggcgtg ccgcccgagc agcgcgagcg catcttcgag
     8521 cccttctacc gcctgcccgg cgccagcgag cgcgaaggcg gcgtgggcct ggggctggcc
     8581 ctggtgcgct ccatcgccca gcgccacggc ggcagcgtca cctgcgacca ccgacccggc
     8641 ggcggggcgc gattcacgct gcggctgccg gtggaggcgg acgcggcgac aaaatagttc
     8701 ttttgttgat taattgcgca aaatcccgca aatgggggac tggtcgaagg gcgatccgcg
     8761 tatacagttg gcgacattgc tgtcacacgg gtgagtttgc gtgaaccgct ctcgcaaagg
     8821 cccggcaaaa aaatcaagaa cccgggctcc gtcacccccc aaggccgcca tcaggcggcc
     8881 ttgttttttg catcggctta caccgcggtc gttcggggcg ccacctacaa ttgccgccca
     8941 tgccgcaact ccccatggtt cgttgggtcg cgctgttctg gggattcgtt gccttcttcc
     9001 cgatcggcct gaactacacc gccttcgtcc tgctggcggt ggccatggtg tggcagggcg
     9061 atcgggccga gcgcctggcg cgcctgcggc aggcgcccca gtggtggccg gcctgggccc
     9121 tcgtgggctg gagcacgctg attttgctgc tggggccgca ctatgcggag accccatcca
     9181 acgccctgca cgcgctgcgc atcgtgctga cgctggcgct ggcgctggcg ctcacgcgcg
     9241 acgaggcgct gtgggcgctg cgcggcgtgc tgctggggct ggccgtcggc ctgctgctgg
     9301 tggcactcca tgggctggtg ccgttgccgc cccacccgct gtgggacagc ctggtgcgct
     9361 acgacggcaa caagtcgctg gccaacgcag tgcagatggc gatggtggcg gcgggcagcc
     9421 tgctgctgct gccggccctg ggccggcgcg gtcaggtgct ggcggcactg ctggccgccg
     9481 ccgtgatgct ggtgctgatc tgggtgctgc attcgcgcac cgcctggctg gtgctggtgc
     9541 tcgggctggc cctggggctg tggcaccgcc tgcgcggtcg gcggcgccct cagctggcgg
     9601 cgctggcgac ggcggcgctg ctgacgctgg cggccggcct gctgctgccg ggcgtcaagc
     9661 agcggctgca gcagggcgcg ggggaactgg tcagtgccta ccaggggcag acggtcgaga
     9721 tggacagcag ctggggcatc cgctaccgca tgtaccggga aaccacggcc atgatcgccg
     9781 agcggccgtt gctcggctgg ggcattggcg gttggaacac gcagtggagg cagcgggtga
     9841 acggcccgct caccgacgtc aacatgccgc acaacgatct tctgtggatg ggcgctcaga
     9901 ccggggtgcc gggtgcgctg ctgctgctgt ggctgctgct ggccggctgg ccggcggctt
     9961 ggcggcgtga cgatctgaac ggcagcctgg cggcgctggc gctgctggcc atgctgctgg
    10021 cggtgctggg caactcggcg ctgcgcgacg cctccatggg cctgagcctg tggttcgtgg
    10081 tgctggtgat ccagcggctg gcccatcagc cgcagcctct ttggcagggt ctgtggccgg
    10141 cgggcgcggt ggcgccgcag gcgggccgct gagcgctggt gcaacgcggc cgcagtgccg
    10201 ggccgcgccg gcagtgtcag tggccggcgc cgaccacctc gccggccttc atgcggtaca
    10261 gggtgccgca gtaagggcat cgggcttcgc cctgggccag gttgaggaac acgcgcgggt
    10321 gggtgttcca gagcttcatg tcggccttgg gatgggggca gaaggtctcg cccgagcggg
    10381 tcaggtcgcg ggccagcagt tcgacgactt ggttgggcgt gctcatgcgc gatgctcctt
    10441 gttgacccgg ctcagacctt ggccagccag tgggcgtact tggcgttgcg gccgttgacg
    10501 atgtcg
//
LOCUS       DAOOOG010000087        10459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_7246_length_10459_cov_25.167339, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000087 DAOOOG010000000
VERSION     DAOOOG010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7246_length_10459_cov_25.167339"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="thiD"
                     /locus_tag="PK242_07815"
     CDS             <1..849
                     /gene="thiD"
                     /locus_tag="PK242_07815"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOZ93961.1"
                     /translation="QQYVRVLTIAGSDSGGGAGIQADLKTFAALGCYGMSAITALTAQ
                     NTQGVTAIHGVPPDMLAAQIDAVLNDIGAQAVKIGMLHAPDIVHTVAAAIERHHMTQV
                     VLDPVMVSATGARLIEAEAVQALVTALFPRASLITPNLDEAALLLGRPVQAVADMAPA
                     AQALLALGARAVLLKGGHLKGQQVHDLLLVRGAEPLHMCSARLATRNLHGAGCTLSSA
                     IAAHLALGLGLSDAVQAARHYVQQALAAGATVQTGEGVGPLNHGFAPRTMVTLGRDQI
                     TSKDVT"
     gene            981..1805
                     /locus_tag="PK242_07820"
     CDS             981..1805
                     /locus_tag="PK242_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ93962.1"
                     /translation="MADSRIDPMPAPAPRAVWIDLRCMPQPHGLYQDARPHGVMARIT
                     QLADIVGAIARWRPHFVCIDYDYPDGARLDAVPRVRREFPQLPVLMFTDYHTEALAVW
                     ALRHRVWDYRVKPVTVSTLRRLFEVLRCGGAEARAPGGWPAPALPPELREPAGHLRKS
                     LQASARTAAAVAWIGEHYGEPCPIKLMAELCHLSPSEFSRAFHREQGVPFCHFLQHHR
                     VTMAREFLREPGATVSQVAYAVGFNDLSYFGRVFRRLVGVPATQYQRLKAPPQGKA"
     gene            1975..3288
                     /locus_tag="PK242_07825"
     CDS             1975..3288
                     /locus_tag="PK242_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93963.1"
                     /translation="MPDTKESSLARIECCPVLERQPVCDRLDFRYRLPYPVRTGDRQT
                     VRVDLIVHFRLERCSGPLVLGDPVYSTTLLPGEQVRLFSSDRHTRWSYDAESKLAYRH
                     ETTSEESFFTYGMARAVSDLTITEGGSASSSYDESWAEGGGGASVNLFGIIEIGGGGG
                     GGSYDAHSASSFARFLSQHAQSASSSVAAGVRAKSSTAVGEVSTREHREGESSDHFES
                     ASRMFRNPNRCHAVTYLFYRVSKVQKLRFRLVAIQRVVSDPAAPVLSDQRPSVDVAGR
                     ISVLPEVVSASSPQRLAIEERARASALSRLNMTGGNVSLLARPLVGAAAPLGVDVRQA
                     ALAAVDKELAAAGVIDPKTGQVHDKLVAELSWEREEVLPTPGILVKGCLDDCATCEPA
                     LQREIELELERKRLDNEKLKREIELLDQAQQYRCCPVGEAEDDDD"
     gene            3367..5565
                     /locus_tag="PK242_07830"
     CDS             3367..5565
                     /locus_tag="PK242_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93964.1"
                     /translation="MARNPDNAGGSGKVELSVHVQLQEVDDRQAAPQPAVYAYSAGGK
                     LLAMQPADERGQARLALAVGAEDTAVRVLVGPTLERPEASELLRRGAVARPLRLSLKD
                     PRPKLVLPIDPDSWRCWLLGRCVVRGTLLKRVVSGGLPLELPVCHASVDVWEVDPWPV
                     LVPRLPPWVIDGLRRVIVEPRPPWPEPPGPWPIDGPGPLPPPGPGPDPVPLAQRRAMR
                     AGRTPGIGSGAAVLASFHAAPQAGELKTLAQIGNAQQFQQALIRHPELVRPLLCWFYP
                     HIVTKQKVATAVTDECGQFRAVFWRGCQSSDVPDLYFTAQQRLFGWLNVSIYEPTPVP
                     CHTHWNYVCGTPVTLITTSPWAIACSPCRPYHPGDNWVLFTAVGNTSLKAIHGGGAPG
                     ATGDNLGLLQSGAPWGGVLRPRLDFDNALRESLGVRYYQLSWRKGMAGDFKPLSVEVN
                     RHYAHMVGTDLVIDPYRLGPNHMVVGDQTLELYEIPPALPPFGQWTIANAVLDTENGE
                     FDSRVFSRGLAFEEDGDVVPGSVDESGLYQIKLDLYDAAGNQIDIAAKGIDYYVPDSV
                     SLSGSIPTVKASSVTQPGGGSLVQGNSLVLTLHVDNNHCWAGVGAPWTPSGAADPCCG
                     VLRFAPGASVSMPYVAYHPHGFATHAFSVVRSATQILSPSGGVGSFVITRTVHDMMTL
                     NLPAACAGKPECTTAAFGERLDVYEMATDGWGSDLGYEASDLRAFALSTH"
     gene            5768..6811
                     /locus_tag="PK242_07835"
     CDS             5768..6811
                     /locus_tag="PK242_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOZ93965.1"
                     /translation="MLIFTTAEQADHDPAHEIFRGQRVPCFENPSRAAFVEQALRAAG
                     HTLRRPDTDSADLLSQVHSPRYLQFLRTAWDQWLALDAANAGVQPFPSVWPVRTLRSD
                     VEPANFIARLGLYSMDNGSPMVAGTWVAAKAAADAAASAARALGQGERAVFCATRPPG
                     HHAGPDFMGGYCFINHAAVCAQALRAGGAQRVAVLDVDYHHGNGTQAIFYERADVLFV
                     SIHGDPRTEYPFYLGHADETGAGAGQGCNLNLPLAAGASVATWFDALDTACARVAAHR
                     PDALVVSLGLDTFAGDPISRFQLQTGDFRRLGRRLQQLGLPTAFILEGGYAAAELGQN
                     AAQVLQGFEEGGR"
     gene            6808..7941
                     /locus_tag="PK242_07840"
     CDS             6808..7941
                     /locus_tag="PK242_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOZ93966.1"
                     /translation="MSVEQVDCVVIGAGVVGLAVARALALTGREVLVLEAEETFGTQT
                     SARNSEVIHAGIYYPPGSLRAGLCVAGRQLLYDYCAARGVQHRRCGKLIVAANAAQQA
                     ELRAIQARAVANGVDDLQWLARDQALALEPQLQCEAALLSPSTGIIDSHGLMLALLGD
                     LEHAGGTLATHSRVNRLVALDGQAPAAIKIEVSSDQETTVLHARSVVNCAGLGAIALT
                     HATQGLAPAHRPPLPQRWAKGSYFTLSGRAPFARLIYPVVERDSPSLGVHLTLDLGGQ
                     AKFGPDVQWVASPDDLQVDPARADAFYAEVRRYWPALPDGALQPGYVGYRPKLSGPGE
                     PAVDFRIEGPGQHGVPGLVNLLGIESPGLTSCLAIARHVVGVL"
     gene            complement(8030..8857)
                     /locus_tag="PK242_07845"
     CDS             complement(8030..8857)
                     /locus_tag="PK242_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOZ93967.1"
                     /translation="MTTTAIDQDRDRTAPPRAPVRVMQIIAALANHRDGLSLAQLSDV
                     LQLPKTSLFSLLRALEEGGYVMSENGHHRLGQETYSLAALIQKIDGFPGNVRSWLNEL
                     HQACQETVLVGVPSKDWTQLIYVDVIEASSWLRYSANVGARRPLYSTSLGLTMLAFTS
                     TERQRHYIESTRLDAVTHHTITSRSALQQALRRIRAAGCACSSGSIEGATGVSAPVFD
                     RDGSLLGAVGLAGPTARYERNADQFRALVVRCGEQMSRALGFTGAYPNVASHPPGPR"
     gene            8992..10158
                     /locus_tag="PK242_07850"
     CDS             8992..10158
                     /locus_tag="PK242_07850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ93968.1"
                     /translation="MSFESTPEQQAIREAVLQSCAPFGEDYWLEHDRTGEFPHQFYAA
                     MAQAGWLGMAIPEAYGGSGLGVTEAALMMQTVAETGAGMSGCSAFHINIFGLMPIVVF
                     GTEAQKQRFLPPMVQGQVKSCFGVTEPNTGLNTTQLKTRAVLKGDRYVVHGQKVWIST
                     AQVADKILLLARTTPIEECKSHSHGLSLFYTDLDRNKVAVRLIEKMGRKAVDSNELFI
                     DGLEIPVEDRIGEEGQGFKYILHGMNPERILIGSEAIGIGRAALRKAATYAQERVVFD
                     RPIGKNQGIQHPLAANWMELEAAHLMMLKAAWLYDQGRDCGAEANATKYLGAEAGFHA
                     CERAILTHGGFGYAKEYHVERYLREVMIARIAPVSREQILSYIAERVLGLPKSY"
     gene            10167..>10459
                     /locus_tag="PK242_07855"
     CDS             10167..>10459
                     /locus_tag="PK242_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOZ93969.1"
                     /translation="MGTPTDILTPERSWLEALAQGRFLLQRSASSGQFVFPPRVAEPR
                     SGATDLQWVEASGAGVVHAVTVISPKPPAAPYTVALIDLAEGVRVMGRVEGLPP"
BASE COUNT         1595 a         3583 c         3674 g         1607 t
ORIGIN      
        1 cagcaatacg tgcgcgtgct caccatcgcc ggcagcgact cgggcggcgg cgccggcatc
       61 caggccgacc tgaaaacctt tgccgccctg ggctgctacg gcatgagcgc gatcaccgcg
      121 ctgacggcgc agaacaccca gggcgtgacg gccatccacg gcgtgccgcc ggacatgctt
      181 gccgcccaga tcgacgccgt gctgaacgac atcggcgcgc aggcggtgaa gatcggcatg
      241 ctgcacgcgc ccgacatcgt gcacaccgtg gccgccgcca tcgagcgcca ccacatgacc
      301 caggtggtgc tggacccggt gatggtgtcg gccaccggtg cgcggctgat cgaggccgag
      361 gccgtgcagg cgctggtgac cgcgctgttt ccgcgcgcca gcctgatcac ccccaacctg
      421 gacgaagcgg cgctgctgct gggccggccg gtgcaggcgg tggccgacat ggcgccggcg
      481 gcgcaggcgc tgctggcgct gggcgcacgc gccgtgctgc tcaagggcgg ccacctgaaa
      541 ggccagcagg tgcatgacct gctgctggtg cgcggcgccg agccgctgca catgtgctct
      601 gcgcgtctgg ccacgcgcaa cctgcacggg gccggctgca cactgtcgtc ggccatcgcc
      661 gcccacctgg cgctgggcct gggcctgagc gacgcggtgc aagccgcccg ccactacgtg
      721 cagcaggcgc tggcggccgg tgccacggtg cagaccggcg agggcgtggg gccgctgaac
      781 catggctttg cgccccgaac catggtgacc ctggggcgcg atcagattac ttccaaagac
      841 gtaacttgat acgagtcttg tgtacctaat acatacaccg atgaggatgc ttcctaacat
      901 ccggctcagg ctgcgtcgct gacgacccgg cccgtccagg caagcgccgg gtcaggcgcc
      961 cggtcctaca ggagcagccc ttggccgaca gtcgcatcga tcccatgcct gcgcccgcgc
     1021 cgcgcgcggt gtggatcgat ctgcgctgca tgccacagcc gcatgggctg taccaggatg
     1081 cccggccgca tggcgtcatg gcccgcatca cgcagctggc cgacatcgtt ggcgccatcg
     1141 cgcgctggcg gccgcacttc gtctgcatcg actacgacta ccccgacggc gcgcgcctgg
     1201 acgccgtgcc gcgcgtgcgg cgcgagttcc cgcagctgcc ggtgctgatg ttcaccgact
     1261 accacaccga ggcgctggcc gtgtgggccc tgcgacaccg ggtctgggac taccgggtca
     1321 agccggtcac cgtcagcacg ctgcggcgtc tgttcgaggt gctgcgctgc ggcggcgccg
     1381 aggcgcgcgc ccccggtggc tggccggcgc cggcgttgcc gccggagttg cgtgagccgg
     1441 cgggccacct gcgcaagtcg ctgcaggcca gcgcgcgcac cgccgccgcc gtggcctgga
     1501 tcggcgaaca ctacggcgag ccgtgtccga tcaagctgat ggccgagctg tgccacctgt
     1561 cgccgtcgga gttcagccgc gcctttcatc gcgagcaggg cgtgccgttc tgtcacttcc
     1621 tgcagcacca tcgcgtgacc atggcgcgcg agttcctgcg cgagcccggt gccacggtgt
     1681 cgcaggtggc ctatgcggtg ggcttcaacg acctgtcgta cttcggccgc gtgtttcggc
     1741 gcctggtggg cgtgccagcc acgcagtatc agcggctgaa ggcgccgccg cagggcaagg
     1801 catagcggct ggccgccctg gccgcaggat cgttgcactc ccgcgcaatt cggtgctaac
     1861 cggcatggcc cgcctcagcc gatgctggag gcggcttccg cgtctcacaa caacaagctg
     1921 tcagggtccg gtctgctgtc gctcatgtcc gccacggcat ttaaggagac catcatgccc
     1981 gacacgaaag aaagctccct tgcaaggatc gagtgctgcc cggtgctcga acgtcaaccg
     2041 gtgtgtgacc ggctcgactt tcgctatcga ttgccctacc cggtgcgcac gggcgatcgg
     2101 cagacggtgc gggtcgatct gatcgtgcat ttccgtctgg agcgctgctc cggcccgctg
     2161 gtgctgggcg atcccgtgta ctcgaccacg ctgctgccgg gcgagcaggt gcggctgttt
     2221 tccagcgacc gccatacgcg ctggtcgtac gacgccgagt ccaagctggc ctatcggcat
     2281 gaaaccacgt ccgaagaatc gttcttcacc tacggcatgg cgcgtgcggt gagcgacttg
     2341 accatcaccg aaggcggctc ggcttcgtcg agctacgacg agtcctgggc cgaaggcggt
     2401 ggcggtgcca gcgtcaacct gttcggcatc atcgagattg gcggcggcgg cggcggtggc
     2461 agctacgacg cgcattcggc cagcagcttt gcgcgttttc tgtcgcaaca cgcgcaatcg
     2521 gcgtcctctt cggtggcggc cggcgtgcgg gccaagagca gcacggcggt cggcgaagtc
     2581 agcacgcgcg agcacaggga gggcgaatcg tccgaccact tcgaatcggc ttcgcgcatg
     2641 ttccgcaacc ccaatcgctg ccacgccgtc acctacctgt tctaccgggt gagcaaggtt
     2701 cagaagctgc gcttccggct ggtggcgatc cagcgcgtgg tcagcgatcc ggcggcgccg
     2761 gtgctgagcg atcagcggcc ctcggtggac gtggccggcc gcatctcggt gctgcccgag
     2821 gtggtcagcg ccagcagccc gcagcgcctg gcgatcgagg agcgggcccg cgccagcgcg
     2881 ctgtcgcgcc tgaacatgac cggcggcaac gtgtcgctgc tggcgcgccc gctggtcggc
     2941 gccgccgcgc cgctgggtgt ggacgtgcgc caggccgcgc tggcggcggt ggacaaggag
     3001 ctggctgcgg ccggcgtgat cgatcccaag accggccagg tgcatgacaa gctggtggcc
     3061 gagctgtcct gggaacgcga agaggtgctg cccaccccgg gcatcctggt caaggggtgc
     3121 ctggacgact gcgccacctg cgagccggcg ctgcagcgtg agatcgagct cgaactcgaa
     3181 cgcaagcggc tggacaacga gaagctcaag cgcgagatcg agctgctcga tcaggcgcag
     3241 caataccgct gctgcccggt cggcgaggcc gaggacgacg acgattgagt cccgcggcgg
     3301 ccggcggccc gtgcgccgcc ggcgccggca agggttggac ggtgaacgca acggaaggag
     3361 aacatcatgg ccagaaatcc tgacaacgcg ggcggctcgg gcaaggtcga gctgtcggtc
     3421 catgtgcagc tgcaggaggt ggacgatcgg caggccgccc cccaaccggc ggtctatgcc
     3481 tattcggccg gcggcaagct gctcgccatg cagccggcgg atgaacgcgg ccaggcgcgg
     3541 ctggcgctgg ccgtcggcgc cgaggacacg gcggtgcggg tgctggtggg gcccacgctg
     3601 gagcggcccg aggccagcga gttgctgcgc cgcggcgcgg tggcgcggcc actgcggttg
     3661 tcgctcaagg acccgcgccc gaagctggtg ctgccgatcg atccggacag ctggcgctgc
     3721 tggctgctgg gccgctgcgt ggtgcgcggg accttgctca agcgcgtggt cagcggcggt
     3781 ctgccgctgg aactgccggt gtgtcacgcc agcgtcgacg tgtgggaggt cgatccctgg
     3841 ccggtgctcg tgccgcggct gccgccctgg gtcatcgacg ggctgcgcag ggtgatcgtc
     3901 gagccgcgcc cgccctggcc ggagccgccc ggaccgtggc cgatcgacgg gccggggccc
     3961 ctgccgccgc ccggccctgg accggacccg gtgccactgg cacagcgccg cgccatgcgt
     4021 gccgggcgca cccccggcat cggcagcggc gccgccgtgc tggcgagctt tcatgccgcg
     4081 ccacaggccg gcgagctgaa gaccctggcg cagatcggca acgcgcagca gtttcagcag
     4141 gcgctgatcc gtcaccccga gctggtgcgg ccgctgctgt gctggttcta tccgcacatc
     4201 gtcaccaagc agaaggtggc caccgcggtg accgacgaat gtggccagtt ccgcgccgtg
     4261 ttctggcgcg gctgccagag cagcgatgtg cctgacctgt acttcacggc ccagcagcgc
     4321 ctgttcggct ggctgaacgt gagcatctac gagcccaccc cggtgccctg ccacacgcac
     4381 tggaactacg tctgtggcac gccggtgacg ctgatcacca ccagcccctg ggccatcgcc
     4441 tgctcgccct gccggcccta ccacccgggc gacaactggg tgctgttcac ggcggtcggc
     4501 aacacctcgc tgaaagccat ccacggcggc ggggcccccg gtgcgacggg cgacaacctg
     4561 ggtctgctgc agagtggcgc gccctggggc ggcgtgctgc ggccgcggct ggacttcgat
     4621 aacgcgctgc gcgaatcgct gggtgtgcgc tactaccagc tgtcctggcg caagggcatg
     4681 gccggtgact tcaagccgct cagcgtcgag gtcaaccgcc actatgcgca catggtcggc
     4741 accgacctgg tgatcgaccc ctaccggctg gggcccaacc acatggtggt gggcgatcag
     4801 accctggagc tgtacgagat tccaccggca ctgccgccgt ttggccagtg gaccatagcc
     4861 aatgccgtgc tggacaccga gaacggagag ttcgacagcc gggtgttctc gcgcgggctg
     4921 gcgttcgaag aagacggcga tgtggtgccc ggcagcgtcg atgagagcgg cctctaccaa
     4981 atcaagctcg atctgtacga cgcggccggc aaccagatcg acatcgccgc caagggcatc
     5041 gactactacg tgcccgacag tgtgagcctg agcggctcga tcccgaccgt caaggccagc
     5101 tccgtgacgc agcctggcgg cggcagcctg gtgcagggca acagtctggt gctgaccctg
     5161 cacgtggaca acaaccactg ctgggccggc gtcggcgcgc cctggacgcc cagcggcgcg
     5221 gccgatccgt gctgcggcgt gttgcgcttc gcgccgggtg cttcggtgtc catgccgtac
     5281 gtggcctacc acccgcatgg cttcgccacc catgcttttt cagtggtgcg cagcgcaacg
     5341 cagatcctgt cgccgtcggg cggcgtcggc agcttcgtca tcacccgcac ggtgcacgac
     5401 atgatgacgc tgaacctgcc ggccgcctgc gcgggcaagc cggagtgcac gacggcggcg
     5461 ttcggtgagc ggcttgacgt ctacgaaatg gccaccgacg gctggggcag cgacctcggc
     5521 tacgaagcgt ccgacctccg ggcattcgca ctgtcaaccc actgagcccg aaggtggccg
     5581 tgggacgcgt gcgcccgaac atgacgggcg gccaacggct gccctcaggc tgagggcagg
     5641 cccgcgcggc cggggtgggc gcgtgccgtc gcccgccccg gtgtcggccc cgccgaggtg
     5701 ctggcgacag cctgggatgc cctaacatgg gcgctcgccg cgcgcacacg cagcccggcc
     5761 gccaccgatg ctgatcttca ccaccgccga acaggctgac cacgacccgg cgcacgagat
     5821 ttttcgtggc cagcgcgtgc cctgctttga aaatcccagc cgcgcagcct ttgtcgagca
     5881 ggccctgcgc gccgccggcc acacgttgcg ccgtccggac accgacagcg ccgatctgct
     5941 gtcgcaggtg cactcaccgc gctacctgca gttcctgcgc acggcctggg accagtggct
     6001 ggcgctggac gcggccaacg ccggcgtgca gccgtttccg tccgtgtggc cggtgcgcac
     6061 gctgcgcagc gacgtggagc ccgccaactt catcgcccgc ctgggcctgt attcgatgga
     6121 caacggcagc cccatggtgg ccggcacctg ggtggcggcc aaggccgctg ccgacgcggc
     6181 agccagcgcc gcgcgggcgc tggggcaggg cgagcgcgcg gtgttttgcg ccacccgccc
     6241 gcccggccac cacgcggggc cggacttcat gggtggctac tgcttcatca accacgccgc
     6301 cgtgtgcgcg caggcgctgc gtgcgggcgg cgcgcagcgc gtggcggtgc tggacgtgga
     6361 ctaccaccac ggcaacggca cgcaagccat tttttacgag cgcgccgacg tgctgttcgt
     6421 cagcatccac ggcgatccgc gcaccgaata cccgttttat ctgggccatg ccgacgagac
     6481 cggcgccggc gcggggcagg gctgcaacct caacctgccg ctggcggcgg gggccagcgt
     6541 ggccacgtgg ttcgacgcgc tggacaccgc ttgcgcgcgc gtggccgccc atcggccgga
     6601 cgcgctggtg gtgtcgctgg ggctggacac cttcgcgggc gacccgatca gccgctttca
     6661 gttgcagacc ggcgacttcc ggcggctggg ccggcgcctg cagcagctgg gcctgcccac
     6721 cgccttcatc ctggagggcg gctacgcggc ggccgagctg ggtcagaacg cggcgcaggt
     6781 gctgcagggc ttcgaggagg gcggacgatg agcgtggagc aggtcgattg cgtggtgatc
     6841 ggcgccggcg tggtcgggct ggcggtggcg cgcgcgctgg cgctgaccgg gcgcgaggtg
     6901 ctggtgctgg aggccgaaga aacctttggc acccagacca gcgcccgcaa cagcgaggtc
     6961 atccacgccg gcatctacta cccgcccggc tcgctgcgcg ccggtctgtg cgtggcgggg
     7021 cggcagctgc tgtacgacta ttgcgccgcg cgcggcgtgc agcaccggcg ctgcggcaag
     7081 ctgatcgtgg cggccaacgc cgcgcagcag gccgagctgc gcgccatcca ggcgcgcgcc
     7141 gtggccaacg gtgtggacga tctgcaatgg cttgcgcgcg atcaggcgct ggcgctggag
     7201 ccgcagctgc aatgcgaggc ggcgctgttg tcgccctcga ccggcatcat cgacagccac
     7261 ggcctgatgc tggcgctgct gggcgatctg gagcacgccg gtggcacgct ggccacgcat
     7321 tcaagggtaa atcgccttgt agcgcttgac ggacaagcgc cagcagctat caaaattgaa
     7381 gtatccagcg accaggaaac caccgtcctg cacgcccgca gcgtggtcaa ctgcgccggc
     7441 ctgggcgcca ttgcgctgac ccacgccacg caggggctgg cgcccgcgca ccggccgccg
     7501 ctgccgcagc gctgggccaa gggcagctac ttcacgctga gcgggcgggc gccctttgcg
     7561 cgcctgatct atccggtggt cgaacgcgac tcgcccagcc tgggcgtgca cctgacgctg
     7621 gacctgggcg gtcaggccaa gttcgggccg gacgtgcagt gggtggcgag ccccgacgat
     7681 ctgcaggtcg atccggcgcg cgccgacgcc ttctacgccg aggtgcggcg ctactggccc
     7741 gcgctgcccg acggggcgct gcaaccgggt tacgtcggct accggcccaa gctctccggc
     7801 ccgggcgagc cggcggtgga tttccgcatc gagggccccg gccagcatgg cgtgccggga
     7861 ctggtgaatc tgctgggcat cgagtcgccg gggctgacca gctgtctggc gatcgcccgg
     7921 cacgtggtgg gcgtgctctg agccaccgcg cagtcgcgcc gcgacggctg ctcgccgtcg
     7981 ccgggcgcgg ggtcgatgtc tgcaaggcga gggggccttc agcggacggt caacgcggcc
     8041 ccggcgggtg gcttgcgacg ttcggatagg cgccggtgaa cccgagcgcg cgcgacatct
     8101 gctcgccgca acgcacgacc agtgcccgaa actgatcggc gttgcgctca tagcgggctg
     8161 tgggcccagc caggccgacg gcacccagca atgagccgtc gcggtcgaac accggagcag
     8221 acacgccggt ggctccctcg atggagccac tgctgcacgc gcagcctgcg gcgcgaatgc
     8281 ggcgcagcgc ctgctgcaga gcgctgcgac tggtgatggt gtggtgcgtc acggcgtcca
     8341 ggcgggtcga ttcgatgtag tggcgctggc gttcggtcga ggtgaatgcc agcatggtca
     8401 ggcccagcga ggtggagtac agggggcgac gcgcacccac gttggccgag taccgcagcc
     8461 aactgctggc ctcgatcaca tcgacataga tcagctgcgt ccaatccttg gaaggcacgc
     8521 ccaccagcac cgtctcctgg caggcctggt gcagttcgtt cagccaggaa cgcacattcc
     8581 cgggaaagcc gtcgatcttc tggatcagcg cagccaggct gtacgtttcc tgccccaggc
     8641 gatggtggcc gttttcggac atcacgtagc cgccttcttc caacgcgcgc agcaggctga
     8701 acaggctggt cttgggcagc tgcagcacgt cgctgagctg cgcgagcgac aggccgtcgc
     8761 ggtggttggc cagggcggcg atgatctgca tgacccgcac gggggcgcgg ggtggtgcgg
     8821 tgcggtcgcg atcctgatcg atggcagtgg tggtcatggc ggcagtgtgg gatcggggtg
     8881 atgcgttcaa agctcgggaa taacccgaag aggcgttgac agcgagggag ctcgattctg
     8941 ataattctct tatcagaaca catattccga tatcagaaca aaccccaagc catgtccttc
     9001 gaatccaccc ccgagcaaca agccattcgc gaagccgtgc tgcagtcctg tgcccccttt
     9061 ggtgaggact actggctcga gcacgaccgc accggcgagt ttccgcacca gttctacgcc
     9121 gccatggccc aggcgggctg gctgggcatg gccattcccg aggcctatgg cggctcgggc
     9181 ctgggcgtca ccgaagcggc gctgatgatg cagaccgtgg ccgagaccgg cgccggcatg
     9241 agcggctgtt cggcctttca catcaacatc tttggcctga tgccgatcgt ggtgttcggc
     9301 actgaagcgc agaagcagcg ctttctgccc cccatggtgc agggacaggt caaatcctgc
     9361 tttggcgtga ccgagcccaa caccggcctc aacaccaccc agctcaagac ccgcgcggtg
     9421 ctgaagggcg accgctatgt ggttcacggg cagaaggtgt ggatctccac cgcccaggtg
     9481 gccgacaaga tcctgctgct ggcgcgcacc accccgatcg aggaatgcaa gagccacagc
     9541 cacggcctgt cgctgttcta caccgacctc gatcgcaaca aggtggcggt gcgcctgatc
     9601 gagaagatgg ggcgcaaggc ggtcgactcc aacgagctgt tcatcgacgg cctggagatt
     9661 cctgtggaag accgcatcgg cgaagaaggc cagggcttca aatacatcct gcacggcatg
     9721 aaccccgagc ggatcctgat cggttccgag gccatcggca ttggccgcgc cgcgctgcgc
     9781 aaggccgcca cctacgccca ggaacgcgtc gtgttcgacc gcccgatcgg caagaaccag
     9841 ggcatccagc atccgctggc cgccaactgg atggagctgg aagccgcgca cctgatgatg
     9901 ctcaaggccg cctggctgta cgaccagggg cgcgactgcg gcgccgaggc caacgccacc
     9961 aagtacctgg gtgccgaggc cggttttcat gcctgcgagc gcgccatcct gacgcacggc
    10021 ggcttcggct acgccaagga gtatcacgtc gagcgctacc tgcgcgaagt gatgatcgcg
    10081 cgcatcgcgc cggtcagtcg cgagcagatc ctgtcgtaca tcgccgagcg cgtgctgggc
    10141 ctgcccaagt cctactgagc gcagacatgg gcactcccac cgacatcctc acacccgagc
    10201 gcagctggct ggaagcgctg gcccagggcc gctttctgct gcagcgcagc gccagcagcg
    10261 gccagttcgt gtttccgcca cgcgtggccg agccgcgcag cggcgccacc gatttgcagt
    10321 gggtcgaagc cagtggcgcc ggcgtggtcc atgccgtcac cgtgatttca cccaaaccac
    10381 cggcagcgcc gtataccgtg gcgctgatcg atctggccga gggcgtgcgc gtcatggggc
    10441 gggtcgaagg cctgccgcc
//
LOCUS       DAOOOG010000088        10413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_7291_length_10413_cov_31.132941, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000088 DAOOOG010000000
VERSION     DAOOOG010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10413
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7291_length_10413_cov_31.132941"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PK242_07860"
     CDS             <1..594
                     /locus_tag="PK242_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOZ93970.1"
                     /translation="RGDRVKGPIKSITDFGVFVGLAAGIDGLVHLSDLSWNEPGEAAV
                     RNYKKGQEVEAIVLAVDVDRERISLGIKQLDQDPFTTFVSVNDKGQTVTGTVKTVDAR
                     GAEIDLGNDVVGYLRASEISRDRVEDASQVLKVGDEVTAVVINVDRKSRNIQLSVKAK
                     DAADQQEAMASLSQSSGSAGTTSLGALLRAKLDGNDK"
     gene            642..974
                     /locus_tag="PK242_07865"
     CDS             642..974
                     /locus_tag="PK242_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HOZ93971.1"
                     /translation="MTRSDLVELLAERFSQLTQRDADAAVKTILDAMSDALARGHRIE
                     IRGFGSFSINHRPPRLGRNPRTGDRVHIPERRVPHFKPGKALREAVGAQPVSPGVRGA
                     PEDRETPH"
     gene            1002..1295
                     /locus_tag="PK242_07870"
     CDS             1002..1295
                     /locus_tag="PK242_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOZ93972.1"
                     /translation="MNRLARLLTWILKAAVFFALFAFALNNQQGATVHLLFGHQWRAP
                     MTLIVLVAFGIGLIVGVLGMLPGWWSRRHGRTPMPGPVPAPSEPSVPEPPHGV"
     gene            1285..2445
                     /gene="lapB"
                     /locus_tag="PK242_07875"
     CDS             1285..2445
                     /gene="lapB"
                     /locus_tag="PK242_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HOZ93973.1"
                     /translation="MEFDLGWILFGLPVVFALGWFASRLDLRQLRIENRQAPKAYFKG
                     LNHLLNEQQDQAIDAFIEAVQKDPDTSELHFALGNLFRRRGEYERAVRVHEHLLSRAD
                     LSQADRDRAQHALALDFLKAGLLDRAEDALRKLEGTAFEEQAGLTLLSLHERARDWPQ
                     AGALARRLQQRGAGDFTLRLSHYQCEQASEALNKKSDRTAAQQYLRLAIDQAPHAARP
                     RIELAGLLAREGDAADALDQLASLEQHAPQAIALVASELVRIGQACGQATRVRELLQR
                     SYASTPSIDVADAIVQADVTAGVPQAQARSGYLRHMAQEPSLIAAARWLSHEGFAQQD
                     AHALVQRSVDHAARPLARYRCAACGFEAQGYFWQCPGCQAWESFPPRRIEEL"
     gene            2565..2945
                     /locus_tag="PK242_07880"
     CDS             2565..2945
                     /locus_tag="PK242_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOZ93974.1"
                     /translation="MLKKILVLLSLLYAAFAFAAVDANTASAADLDSVKGIGPGMSSR
                     IIKERKKSEFKDWADFISRVKGVGEKNAAKFSAAGLTVNGAAYTAAAAASPAKPKAAP
                     AAASSNTAAKTPAPSAPATAASKP"
     gene            complement(3204..6893)
                     /locus_tag="PK242_07885"
     CDS             complement(3204..6893)
                     /locus_tag="PK242_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HOZ93975.1"
                     /translation="MSEHALHATRWQFWIDRGGTFTDIVARRPDGTLVTHKLLSDNPE
                     QYPDAAVAGIRQLLGLASGVPITPAQVACVKMGTTVATNALLERKGEPTLLVTTCGFR
                     DALRIAYQARPRLFDRHIVLPELLYAAVVEASERVDADGAVVLPLDEVLLKQELLAQY
                     QSGLRSVAIVFIHGWKHVAHEQAAARLAREIGFTQVSTSHQTSPLMKFVSRGDTTVVD
                     AYLSPILRRYVDRVAAEMPGVPLYFMQSSGGLTDAHAFQGKDAILSGPAGGIVGMART
                     AQLAGQQRVIGFDMGGTSTDVSHFAGQFEREFETQVAGVRMRAPMMSIHTVAAGGGSI
                     LRFDGTRFRVGPESAGANPGPASYRRGGPLALTDANVMVGKLQPAYFPRVFGPAADAP
                     LDAEVVRARFAEIAAQDAAGGRTSEEVAHGFIQIANQQMASAIKKISVARGYDVTRYT
                     LQCFGGAGGQHACAVADALGMNRVLVHPLAGVLSAYGMGLADQTLMRERALELPLEIA
                     QLPALRAAGDALAKEARAQLQGQHAGGGAIQVHQRLHLRYAGTDSALVVAFDRALDGA
                     GAAAALQAITQAFEAAYRQRFAFLMQGKALIVEAVSVEAVIAGEAPAEIEQPLQPPRS
                     VPRRATVRIYTSGAGGQSAWHEAALIVREDLRAGDVIEGPAIIAERNATTVLEPGWRA
                     RLTGLDHLLLERAAPRAARFAAGTQVDPVLLEVFNNLFMNIAEQMGLQLQNTAYSVNI
                     KERLDFSCALFDAAGHLIANAPHMPVHLGSMGESIRAVIARNPDMQRGDVYVLNDPYH
                     GGTHLPDITVITPVCLDGEARPVFYVGSRGHHADIGGITPGSMPPFSTRIEEEGVQID
                     NVKLVDRGRLCEREVLELLASGEYPSRNPLQNLADLKAQIAANEKGVQELRKMVEQFG
                     LDVVQAYMQHVQDNAEESVRRAITRLAARVTNGRFTLPLDNGAQIQVAVAIDAATRSA
                     KIDFSGTSPQQSNNFNAPTAVCMAAVLYVFRILVDDDIPLNAGCLKPIQVVIPEGSML
                     HPQAPASVVAGNVETSTCITNALIGALGLMGASQCTLNNFTFGNERYQYYETISGGSG
                     AGGEFDHQGGLVGGFDGTSVVQTHMSNSRLTDPEVLEWRFPVRLESYEIRHGSGGTGR
                     WRGGDGGVRRLRFLEPMTASILSNGRVHGAFGMAGGGAGAVGINRVLRADGSVQELQH
                     IGSVEMQAGDIFEIHTPGGGGYGAR"
     gene            7132..8118
                     /locus_tag="PK242_07890"
     CDS             7132..8118
                     /locus_tag="PK242_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOZ93976.1"
                     /translation="MTLTRRTLGALAVLSLGGFALSAAAQTKWDLPSAYPASNFHTEN
                     LVQFAKDVDAATGGKLKIQVHDNASLFKAPEIKRAVQGGQAQAGELLMVNFQNEWPVW
                     GLDGIPFLATSYAESKKLYQASKATVQKKLAEQGMVLLYAVAWPPQGIFTKKQVGSAA
                     DLKGIKWRAYSPATARIAELVGAQPVTVQQAELSQAMATGVVESYMSSGSTGYDTKTY
                     EHLKFFADTQAWLPKNAVIANKKAFDALDKATQDGLLKAAADAEARGWATSERKTNEY
                     LDLLRKNGMTVYAPSAQLMTDMRKVGDTMLKEWLDKAGAEGKAVVDAYRAAK"
     gene            8210..8722
                     /locus_tag="PK242_07895"
     CDS             8210..8722
                     /locus_tag="PK242_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOZ93977.1"
                     /translation="MRRLLDFLYDAAAWLAALAMVGVLLMVLTSILGRLLDFHLPGTD
                     AYAGYSMAAAGFLSLAHTLKKNEHIRVTLLMGRLRGGARRGMELWALSVAVLLAGLFA
                     WFSVRLVWQSHLFNDMSTSNDATPLWIPQIAMAVGTVILLLAFIDEWVLELRGLRQHE
                     SGDEEALHNE"
     gene            8715..10010
                     /locus_tag="PK242_07900"
     CDS             8715..10010
                     /locus_tag="PK242_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOZ93978.1"
                     /translation="MSDFAITGLLILSLFALLGSGVWIGLTLTGVAWIGMQLFSARPA
                     GDAMAVTIWGSASSWTLTALPLFIWMGEILFRTKLSESMFRGLAPWVTWLPGRLLHTN
                     VIGCAIFAAVSGSSAATCATIGKMTLPELGSRGYPRDITIGSLAGAGTLGLLIPPSII
                     MIVYGVTADVSIAKLFIAGILPGILLAALFSGYLMVWALLHPAQIPMPDRRLSLAEKL
                     HESRHLIPVVLLIAAVLGSIYSGVATATEAAAVGVIGSLLLSLVQGSLSWSTFKTALL
                     GATRLYCMIALILAGAAFLTLAMGYIGLPRHLAEFIGGLGLSPFMLVLALAVFYIVLG
                     CFLDGISMVVLTMGVILPTVQAAGLDLIWFGIFIVIVVEMAQITPPVGFNLFVLQGMT
                     RHDIAYIARVTLPFFFLMCAMVLLLWFFPQIATWLPKNM"
     gene            10317..>10413
                     /locus_tag="PK242_07905"
     CDS             10317..>10413
                     /locus_tag="PK242_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOZ93979.1"
                     /translation="MLYRLGPGWPASAAQLEEALAREPFAECGATQ"
BASE COUNT         1614 a         3661 c         3486 g         1652 t
ORIGIN      
        1 cgcggcgacc gcgtcaaggg cccgatcaag tcgatcaccg acttcggcgt gttcgtcggc
       61 ctggccgccg gcatcgacgg cctggtgcac ctgtccgacc tgtcctggaa cgagcccggc
      121 gaagccgccg tgcgcaacta caagaagggc caggaagtcg aggccatcgt gctggccgtc
      181 gacgtcgacc gcgagcgcat cagcctgggc atcaagcagc tcgaccagga cccgttcacc
      241 accttcgtgt cggtcaacga caagggccag accgtcaccg gcaccgtcaa gaccgtggac
      301 gcacgtggcg ccgagatcga cctgggcaac gacgtggtgg gctacctgcg tgcctccgag
      361 atcagccgag atcgcgtgga agacgccagc caggtgctga aggtcggtga cgaagtcacg
      421 gccgtggtca tcaacgtcga ccgcaagtcg cgcaacatcc agctgtcggt caaagccaag
      481 gacgccgccg accagcagga agccatggcc agcctgagcc agagcagcgg cagcgccggc
      541 accaccagcc tgggcgccct gctgcgcgcc aagctggacg gcaacgacaa gtaagccgag
      601 ccgcgcggcc aggggcgccc cctctggccg cgctcaccgc catgacacgc tccgatctgg
      661 tcgaactgct ggccgaacgc ttcagccagc tcacgcagcg cgacgccgac gcggcggtca
      721 agaccatcct ggatgcgatg agcgacgcgc tggcgcgcgg tcatcgcatc gagatccgcg
      781 gtttcggcag cttctccatc aaccaccggc cgccccgtct gggtcgcaac ccacgcaccg
      841 gcgaccgcgt gcacatcccc gagcggcgcg tgccccactt caagccgggc aaggcgctgc
      901 gagaagccgt gggcgcgcag ccggtcagtc ccggcgtacg cggcgcaccg gaggatcggg
      961 aaacaccaca ctagaatctt cttcgacacc gaagaagacc catgaaccga cttgcccggc
     1021 tgctgacgtg gatactcaag gcagccgttt tttttgccct gttcgccttt gccctcaata
     1081 accagcaggg cgccaccgtg cacctgctgt tcggccatca gtggcgtgca cccatgacgc
     1141 tgatcgtgct ggtggccttc ggcatcggcc tcatcgtggg cgtgctgggc atgctgccgg
     1201 gttggtggtc gcgccgccac ggccgcacgc ccatgcccgg ccccgtaccg gcgccgtcgg
     1261 agccttcggt gccggagcct ccccatggag tttgatctcg gctggatcct gttcggcctg
     1321 ccggtggtgt ttgccctggg ctggtttgcc tcgcgcctgg acctgcgcca gctgcgcatc
     1381 gagaaccggc aggcgcccaa ggcctacttc aagggcctca accatctgct gaacgagcag
     1441 caggaccagg ccatcgacgc cttcatcgag gcggtgcaga aagaccccga cacctccgaa
     1501 ctgcacttcg ccctcggcaa cctgtttcgc cgccgcggcg aatacgagcg tgccgtgcgc
     1561 gtgcacgagc acctgctgtc gcgcgccgac ctgagccagg ccgaccgcga ccgtgcccag
     1621 catgcgctgg cgctcgactt cctgaaggcc gggctgctgg accgcgccga agacgccctg
     1681 cgcaaactcg agggcacggc gttcgaggag caggccggcc tgaccctgct gtcgctgcac
     1741 gagcgggcgc gcgactggcc gcaagccggg gccctggccc ggcggttgca gcagcgcgga
     1801 gcgggcgatt tcaccttgcg cttgtcacac taccaatgcg agcaggccag cgaggcgctg
     1861 aacaagaaga gcgatcgcac cgccgcccag caatacctgc gcctggcgat cgaccaggcc
     1921 ccgcatgcgg cccgaccacg catcgagctg gccggcctgc tggcgcgcga gggcgatgcc
     1981 gccgacgcac tcgatcagct ggccagcctg gagcagcacg cgccgcaggc cattgcgctg
     2041 gtggccagcg aactggtgcg catcggacag gcctgtggac aggccacgcg cgtgcgcgaa
     2101 ctgctgcagc gcagctacgc cagcacgcca tcgatcgacg tggccgacgc catcgtgcag
     2161 gccgacgtca ccgccggtgt gccccaggcg caagcgcgct ccggctacct gcgccacatg
     2221 gcccaggagc cgtcgctgat cgcggcggcg cgctggctca gccatgaagg ctttgcccag
     2281 caggacgccc acgccctggt gcagcgctcg gtcgaccacg ccgcccggcc tctggcgcgc
     2341 taccgctgcg cggcctgcgg cttcgaggcg caggggtatt tctggcaatg tccgggctgc
     2401 caggcctggg agagctttcc gccgcgccgc atcgaggagc tgtgaccgct gggcgacccg
     2461 cgccgcggct gtggtagtgt caacgccgac accgcccgat cagttgaata ccggcccgcc
     2521 ggcggcctca gccggcatgc tttcgtcacc aggagtgttc gcccatgttg aagaagatcc
     2581 tcgtccttct gagcctgctc tacgccgcct tcgcctttgc cgcggtcgac gccaacaccg
     2641 ccagcgccgc cgacctggac agcgtcaagg gcatcggccc cggcatgtcc agccgcatca
     2701 tcaaggaacg caagaagagc gaattcaagg actgggccga cttcatcagc cgcgtcaaag
     2761 gcgtgggcga gaagaacgcc gccaagttct ctgccgctgg cctgacggtc aacggcgccg
     2821 cctacaccgc agcggccgcg gccagccccg ccaagccgaa agcggcgccg gcggcagcga
     2881 gcagcaacac cgcggccaag accccggccc ccagcgcacc cgcaacggcc gccagcaaac
     2941 cctgacgccc acgcgcccgc cgggcgccct gacccctcgg acggcacgcc agcgcgtgcc
     3001 gttttctttg cgcttgatta ctacgatttt gatagcttgc ggcgcagttc catcaagcgc
     3061 caggagccga tttggttgat gatcctgtga acgaccgtcg aacctagaag cggcagttgg
     3121 acgcgcccgc cggtgccgtg atcggcgtgc caggcaaggg cgatcgcgca gcctgctcac
     3181 cgcacttcgg aagtgatgat ggatcagcgc gcaccatagc cgcccccgcc cggtgtgtgg
     3241 atttcgaaga tgtcgccggc ctgcatctcc acgctgccga tgtgctgcag ctcctgcacg
     3301 ctgccgtcgg cgcgcagcac ccgattgatg cccaccgcgc ccgccccgcc gcccgccatg
     3361 ccgaaggcgc cgtgcacgcg gccgttgctg aggatgctgg cggtcatggg ctcgagaaag
     3421 cgcaggcggc gcacgccccc atcgccgccg cgccagcgcc cggtgccgcc cgagccgtgg
     3481 cggatctcgt agctctccag gcgcaccgga aagcgccact ccagcacctc ggggtcggtc
     3541 aggcgcgaat tgctcatgtg cgtctgcacc acgctggtgc cgtcaaagcc ccccaccagc
     3601 ccaccctggt ggtcgaactc accgcccgcg cccgagccgc cggagatggt ctcgtagtac
     3661 tggtagcgct cgttgccgaa ggtgaagttg ttgagcgtgc actggctggc gcccatcagg
     3721 cccagcgcgc cgatcagcgc gttggtgatg caggtcgagg tctcgacgtt gcccgccacc
     3781 acgctggccg gcgcttgtgg gtgcagcatc gaaccttcgg ggatcaccac ctggatgggc
     3841 ttgaggcagc cggcgttgag cggaatgtcg tcgtccacca ggatgcggaa cacgtacagc
     3901 accgccgcca tgcacaccgc ggtgggcgcg ttgaagttgt tgctctgctg aggcgaagtg
     3961 ccggaaaaat cgatcttcgc gctgcgcgtg gcggcgtcga tggcgacggc cacctggatc
     4021 tgcgcgccgt tgtccagcgg cagggtgaag cgcccattcg tcacccgagc ggccagacgg
     4081 gtgatcgccc ggcgcacgga ttcctcggcg ttgtcctgca cgtgctgcat gtaggcctgc
     4141 acgacatcca ggccgaactg ctcgaccatc ttgcgcagct cctgcacgcc tttttcgttg
     4201 gcggcgatct gcgccttcag gtcggccagg ttctgcagcg gattcctgct cgggtattca
     4261 ccactggcca gcaactccag cacctcgcgc tcgcacagcc gcccgcgatc gaccagcttg
     4321 acgttgtcga tctgcacgcc ctcctcctcg atgcgggtgg aaaacggcgg catcgagccg
     4381 ggcgtgatgc cgccaatgtc ggcgtggtgg ccgcgcgagc cgacgtagaa cacgggccgc
     4441 gcctcgccgt ccagacacac cggcgtgatc accgtgatgt cgggcaggtg cgtgccgccg
     4501 tggtaggggt cgttcagcac atagacgtcg ccgcgctgca tgtcggggtt gcgcgcgatc
     4561 acggcccgga tgctctcgcc catgctgccc aggtgcaccg gcatgtgcgg cgcgttggcg
     4621 atcaggtggc ccgcggcgtc gaacagcgcg cacgagaagt ccagccgctc cttgatgttg
     4681 accgagtagg cggtgttctg cagctgcagg cccatctgct cggcgatgtt catgaacagg
     4741 ttgttgaaca cctccagcag caccgggtcg acctgggtgc cggcggcaaa gcgtgccgcg
     4801 cgcggggcgg cgcgctcgag cagcaggtga tccagccccg tcaggcgggc gcgccagccg
     4861 ggctcgagca cggtggtggc gttgcgctcg gcaatgatgg ccgggccctc gatcacgtcg
     4921 ccggcgcgca ggtcctcgcg cacgatcagc gctgcctcat gccaagcgct ttggccgcct
     4981 gcgccgctgg tgtagatgcg cacggtggcg cgccgcggca cggagcgcgg cggctgcagc
     5041 ggctgctcga tttcggcagg ggcttcgccg gcgatcaccg cctcgaccga caccgcctcg
     5101 acgatcagcg ccttgccctg catcaaaaag gcaaagcgct ggcggtaggc cgcctcgaaa
     5161 gcctgggtga tggcctgcag cgcggcggcg gcgcccgcgc cgtcgagcgc gcggtcgaag
     5221 gccaccacca gggccgagtc ggtgccggcg tagcgcaggt gcaggcgctg gtgcacctgg
     5281 atggcgccgc caccggcgtg ctggccttgc agctgcgcgc gcgcctcctt ggccagggcg
     5341 tcgccggcgg cgcgcagcgc gggcagttgc gcgatttcca gcggcagctc cagcgcacgc
     5401 tcgcgcatca gcgtctggtc ggccaggccc atgccgtagg ccgacagcac gcccgccagc
     5461 gggtgcacca gcacgcggtt catgcccaag gcatcggcca cggcgcaggc gtgctggccg
     5521 ccagcaccgc caaagcactg cagggtgtag cgcgtcacgt cgtagccgcg cgccacgctg
     5581 atcttcttga tggcgctggc catctgctgg ttggcgatct ggatgaagcc gtgcgccacc
     5641 tcctcggacg tgcgcccgcc ggcggcgtcc tgcgcggcga tctcggcaaa gcgcgcgcgc
     5701 accacctcgg cgtccagcgg cgcatcggcc gcggggccga acacgcgcgg aaagtacgcg
     5761 ggctgcagct tgcccaccat cacgttggcg tcggtcagcg ccagcggccc gccgcgccgg
     5821 tagctggccg ggcccgggtt ggcgccggcg ctttcgggac ccacgcgaaa gcgcgtgccg
     5881 tcaaagcgca ggatcgaccc gccgcccgcg gccacggtgt ggatgctcat catcggcgcg
     5941 cgcatgcgca cgccggccac ctgggtctcg aactcgcgct cgaactggcc ggcaaaatgg
     6001 ctcacgtcgg tcgaggtgcc gcccatgtcg aagccgatca cgcgctgctg ccccgccagc
     6061 tgcgcggtgc gcgccatgcc gacgatgccg ccggccggcc cggacaggat ggcgtccttg
     6121 ccctggaagg cgtgagcgtc ggtcaggccg cccgaggact gcatgaagta caggggcaca
     6181 cccggcatct cggccgccac ccgatcgacg tagcgccgca ggatgggcga caggtaggca
     6241 tcgaccaccg tggtgtcgcc gcggctgacg aacttcatca gcgggctggt ctggtggctg
     6301 gtcgagacct gggtgaagcc gatctcgcgc gccaggcgcg ccgccgcctg ctcgtgcgcg
     6361 acgtgcttcc agccgtggat gaacacgatc gccacgctgc gcagaccgct ctggtactgc
     6421 gcgagcagct cctgtttcag cagcacttcg tcgagcggca ggaccaccgc gccatcggca
     6481 tccacgcgct cgctggcctc gaccacggcg gcgtacagca gctcgggcag cacgatgtga
     6541 cgatcgaaca ggcgcgggcg cgcctggtag gcgatgcgca gcgcatcgcg aaagccgcac
     6601 gtggtcacca gcagcgtggg ttcgcccttg cgctcgagca gcgcgttggt ggccaccgtg
     6661 gtgcccatct tgacgcaggc cacctgcgcc ggcgtgatgg gcacgccgct ggccagcccc
     6721 agcagctggc gaatgccggc caccgccgca tcggggtact gctcggggtt gtccgacagc
     6781 agcttgtgtg tcaccaaggt gccatcgggg cggcgcgcca cgatgtcggt gaaagtgcca
     6841 ccgcggtcga tccagaactg ccagcgtgtt gcgtggagag catgttccga catcatgatc
     6901 tttcaataaa acagggttct aacgcacaac tggcgtgcgc aataagctat ctagttcata
     6961 gcactccggg cgccctcggc agcacggcag cggcccgccc atgaggccgc attattcgcg
     7021 ccgtgccgcg ccacggcgcg cggcggcggg gtggtgtttc cacggtttgc gcgggcgccg
     7081 ccagcgtcta tgctcgcggc acgccttttt cattcccatc aaggagttga catgaccctg
     7141 acccgacgca ccctgggcgc cctcgccgtg ttgagcctgg gcggcttcgc cctttcagcg
     7201 gctgcccaga ccaaatggga cctgccctcg gcctacccgg ccagcaactt ccacaccgaa
     7261 aacctggtgc agttcgccaa ggacgtggac gccgccaccg gtggcaagct gaagatccag
     7321 gtgcatgaca acgcctcgct gttcaaggcg cccgagatca agcgcgccgt gcagggcggg
     7381 caggcgcagg ccggtgagct gctgatggtc aacttccaga acgagtggcc ggtgtggggc
     7441 ctggacggca tccccttcct ggccaccagc tacgccgaat ccaagaagct gtaccaggcc
     7501 agcaaggcga cggtgcagaa gaagctggcc gaacagggca tggtgctgct gtacgccgtg
     7561 gcctggccgc cgcagggcat cttcaccaag aagcaggtcg gcagcgcggc ggacctcaag
     7621 ggcatcaagt ggcgcgccta cagcccggcc accgcgcgca tcgccgagct ggtgggcgcc
     7681 cagccggtga cggtgcagca ggccgagctg tcgcaggcca tggccaccgg cgtggtggaa
     7741 agctacatga gctccggctc cactggctac gacaccaaga cctacgagca cctgaagttc
     7801 tttgccgaca cccaggcctg gctgcccaag aacgccgtga tcgccaacaa gaaggccttc
     7861 gacgcgctcg acaaagccac ccaggacggt ttgctcaagg ccgccgccga cgccgaggcc
     7921 cgcggctggg ccaccagcga gcgcaagacc aacgaatacc tggacctgct gcgcaagaac
     7981 ggcatgacgg tgtacgcccc ctcggcccag ctgatgaccg acatgcgcaa ggtgggcgac
     8041 accatgctga aggaatggct ggacaaggcc ggcgccgagg gcaaggccgt ggtcgacgcc
     8101 taccgcgccg ccaagtaagc gcccgcgcac cccgtggcac actccccccg ggttcgcccg
     8161 gcgggagttt ttttgagccc agaccacgca acccgaccaa ggagccaacg tgcggcgatt
     8221 gctcgatttc ctctacgacg cggcggcctg gctggcggcg ctggccatgg tcggcgtgct
     8281 gctgatggtg ctgacctcca tcctcggccg tctgctcgac tttcacctgc cgggtaccga
     8341 cgcctacgcc ggctacagca tggctgcggc gggctttctg tcgctggcgc acacgctcaa
     8401 gaagaacgag cacatccgcg tgaccctgct gatggggcgc ctgcgcggcg gcgcgcggcg
     8461 cggcatggag ctgtgggcgc tgtcggtggc ggtgctgctg gcggggctgt tcgcctggtt
     8521 ttcggtgcgg ctggtctggc agtcgcacct gttcaacgac atgtccacca gcaacgacgc
     8581 cacgccgctg tggataccgc agatcgccat ggccgtgggc acggtgatcc tgctgctggc
     8641 cttcatcgat gaatgggtgc tggagctgcg cggcctgcga cagcacgagt cgggcgacga
     8701 ggaggcgctg cacaatgagt gacttcgcca tcaccggcct gctgatcctg tcgttgttcg
     8761 cgctgctggg cagcggcgtg tggatcggcc tgacgctgac cggcgtggcc tggatcggca
     8821 tgcagctgtt ctcggcccgc ccggcgggcg acgccatggc ggtcaccatc tggggctcgg
     8881 cctccagctg gacgctcacc gccctgcccc tgttcatctg gatgggcgaa atcctgtttc
     8941 gcaccaagct gtcggaaagc atgtttcgcg gcctggcgcc gtgggtgacc tggctgcccg
     9001 ggcggctgct gcacaccaat gtgatcggct gcgccatctt tgccgccgtg tcgggctcgt
     9061 cggccgccac ctgcgccacc atcggcaaga tgaccctgcc cgagctgggc agccggggct
     9121 acccgcgcga catcaccatc ggctcgctgg ccggcgccgg caccctgggc ctgctcattc
     9181 cgccctcgat catcatgatc gtctacggcg tgacggccga cgtgtcgatc gccaagctgt
     9241 tcatcgccgg catcctgccg ggcatcctgc tggcagcgct gttctcgggc tatctgatgg
     9301 tgtgggcgct gctgcacccc gctcagatcc ccatgcccga ccggcgcctg agcctggccg
     9361 agaagctgca cgaatcgcgc cacctgattc cggtggtgct gctgatcgcc gccgtgctgg
     9421 gttccatcta ttccggcgtg gccaccgcca ccgaggcggc ggccgtgggc gtgatcggtt
     9481 cgctgctgct gtcgctggtg cagggcagcc tgtcgtggag caccttcaag accgcgctgc
     9541 tgggcgccac ccggctgtac tgcatgattg cgctgatcct ggccggcgcc gcctttctga
     9601 cgctggccat gggctacatc ggcctgccgc gccatctggc cgaattcatc ggcggcctgg
     9661 gcctgtcgcc cttcatgctg gttctggcgc tggcggtgtt ctacatcgtg ctgggctgct
     9721 ttctggacgg catctcgatg gtggtgctga ccatgggcgt aatcctgccg acggtgcagg
     9781 ccgccgggct ggacctgatc tggttcggca tcttcatcgt catcgtggtc gagatggcgc
     9841 agatcacgcc gcccgtgggc ttcaacctgt tcgtgctgca gggcatgacc aggcacgata
     9901 tcgcctacat cgcgcgcgtc acgctgccct tcttcttcct gatgtgcgcc atggtgctgc
     9961 tgctgtggtt cttcccgcag atcgccacat ggctgccgaa gaacatgtaa ccccccctgt
    10021 gtcgcctgcg gcgccttccc cctggaggcg ggacgcaccc actggccgag gggaccccgg
    10081 ccacgggagc cctggcttga cctgctccgc ggccattcga cctgtgcgaa gcaagccatc
    10141 tgccactgag ttgcgtggcg acgatgccgg ccctgcctcc tccggggcgt tggcgcctga
    10201 cctgctccgc ggccatcgga cccatgcggg gtgggcgcaa gcgatgtggc taagatgccg
    10261 gtccggcccc ttcgggggtg tttgagacag gttgactatc cgtgttcaag aatctgatgc
    10321 tgtatcgcct gggtcccggt tggcccgcat cggcggcgca gctcgaagaa gcgctggcgc
    10381 gcgagccgtt tgccgagtgc ggcgccacgc agc
//
LOCUS       DAOOOG010000089        10372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_7340_length_10372_cov_32.233886, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000089 DAOOOG010000000
VERSION     DAOOOG010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7340_length_10372_cov_32.233886"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1751
                     /gene="mnmG"
                     /locus_tag="PK242_07910"
     CDS             <1..1751
                     /gene="mnmG"
                     /locus_tag="PK242_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOZ93980.1"
                     /translation="LATDEAGIQFRILNSSKGPAVRATRAQADRVRYKAAIRRRLENQ
                     PNLWLFQQAVDDLLLQGDRVAGAVTQLGIAFRARAVVLTAGTFLDGRIHVGLANHAGG
                     RAGDPPALSLSARLKELKLPQGRLKTGTPPRLDGRTIDFSQCELQPGDGMPGGQNDRM
                     PVFSFMGRADMHPPQMPCWVTHTNARTHEIIKSGFDRSPMFTGVIEGVGPRYCPSVED
                     KVHRFAGKDSHQIFLEPEGLDTHEVYPNGISTSLPFDVQLALVRSMRGLENAHILRPG
                     YAIEYDYFDPRALRSSFETRAIGGLFFAGQINGTTGYEEAAAQGLFAGINAALQCRGE
                     APWLPRRDEAYLGVLVDDLVTQGVTEPYRMFTSRAEFRLQLREDNADMRLTDTGRRLG
                     LIDDARWDAFCRKRDAVSRETERLKSTWVNPRRLPASESERVLGKAIEHEHRLFDLLR
                     RPGVSHEALAGLDGGKYASAEVSRETLGELWAPVIEQVEIAAKYAGYIDRQREEVERA
                     AHFEHLRLPPDLDYMQVPALSIEVRQKLNLHRPETLGQASRISGVTPAAISLLLIHLK
                     KGGFKGFAGGGAAEAA"
     gene            1751..2392
                     /gene="rsmG"
                     /locus_tag="PK242_07915"
     CDS             1751..2392
                     /gene="rsmG"
                     /locus_tag="PK242_07915"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOZ93981.1"
                     /translation="MHPQRAALGAGAQALGLALDERQIDRLLAYLDLLLKWNRVYNLT
                     ALRDPADMLTHHVLDSLALIAPLRAHAGGRALRLLDVGSGGGLPGVVIAIGCPGIDVT
                     CVDAVGKKAAFVQQVAGALGLANLHGQHGRVEAITGRFDVVTSRAFASLADFTAWSDG
                     ALAEGGVWLAMKGRRPDDEIAALPPAVTVFHVEPLTVPGLEAERCLVWLRRAG"
     gene            2421..3035
                     /locus_tag="PK242_07920"
     CDS             2421..3035
                     /locus_tag="PK242_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOZ93982.1"
                     /translation="MFGISDYGAFVAAIVLFLAVPGPGNLALLSSTAQGGVRGGLAAT
                     LGLIAGDQVLLWCAVAGVAALLQSAPTLFAAVQWLGAAYLAWLGWRMLSARPGQGAVL
                     RLAPRHFLRQAFLITLLNPKAIVFYMAFFPLFVDPARHQGLLSFGAMALTIAVLTFLY
                     GLGATLIAHHMAARIRAHPRLSGGLQKLAGVFLIGFGLRLALTK"
     gene            3042..3827
                     /locus_tag="PK242_07925"
     CDS             3042..3827
                     /locus_tag="PK242_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ93983.1"
                     /translation="MAHIFCIANQKGGVGKTTTTVNLAAGLARIGQRVLLVDLDPQGN
                     ATMGSGVDKRAMALSVYDVLLESCSIAEAASVPERLKEAGAGYAVLGANRELAGAEVE
                     LVGEERRERRLKQALAQVDADYDFVLIDCPPSLSMLTLNGLCAAQGVIVPMQCEYFAL
                     EGLADLTNTIRQVHANLNRDLRLIGLLRVMFDARITLQQQVSDQLKAHYGSKVFDTII
                     PRNVRLAEAPSYGLPGVVFDPAAKGSQAFVAFAEELVRRGLSA"
     gene            3872..5002
                     /locus_tag="PK242_07930"
     CDS             3872..5002
                     /locus_tag="PK242_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOZ93984.1"
                     /translation="MKSLTEPRTPPAAGLPEPWIDWGKAIASQLIVWHHLAHYGPLAP
                     RVAAVWPDLMGWLDGRALYAVQAFLVMGGYLAARSLWPRPGAPRVAAADWPARAARRW
                     RRLMPTYLAALLLAVLCAALARQWMDDPDTPAAPTAGQLLAHLLLLQDLLEQPALSAG
                     LWYVAIDLQLYALLAALALLAGRAPARWRRASALALVVGGGAASVLVFNRQPGGDELA
                     PYFFGSYALGVLAAWGRAAGTAAGRRAAGALLLGLAGAGLALAWRDRLALAALCAMLL
                     LWQPGRTRLALAGQPWLRSVVAQLAAISYAVFLLHYPISLLVTALLIDALPAGPVPAA
                     ALLELTWLLSLATGWALTQALQAPGLWQRLRRLTARWRHGTR"
     gene            4989..5579
                     /locus_tag="PK242_07935"
     CDS             4989..5579
                     /locus_tag="PK242_07935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ93985.1"
                     /translation="MAHADAPAATVLTLPGWLGSGPAHWQSLWEQRHGCQRVEQHDWQ
                     HPRRGDWSARLEEVVLAQPAGTPLLLAAHSLGCLLVAWWAAHSRLATRVSGALLVAPP
                     EIERDDLRQQIPGWAPPARQRLPFASLVVASSDDPFGRLAHARALAQDWGAAFRDLGA
                     RGHLNADSGLGDWAQGLALLYALLPSPTCAPGGSTA"
     gene            5590..7020
                     /gene="aspA"
                     /locus_tag="PK242_07940"
     CDS             5590..7020
                     /gene="aspA"
                     /locus_tag="PK242_07940"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOZ93986.1"
                     /translation="MSTPNPNRIEHDLIGERELPNDVYYGVHTLRALENFPITGTAIS
                     IYPDLVRALASIKKAAALANRDLGLLSDAHCQAIGAACDELLAGKLHEQFVVDVIQGG
                     AGTSTNMNANEVIANRALELMGHAKGEFQHLHPNEHVNLGQSTNDVYPSALKIAAWHG
                     ISDLIASMAYLRAAFEAKAEEFKDVLKMGRTQLQDAVPMTLGQEFGTYAVMLGEDEQR
                     LREAQQLICELNMGATAIGTGITAHPDYAAKVIAHLRAITGIPLATAPNLIEATQDCG
                     AFVQLSGVLKRVATKLSKTCHDLRLLSSGPRAGLGEINLPAVQAGSSIMPGKVNPVIP
                     EVVNQIAYEVIGNDVTVTFAAEAGQLQLNAFEPVIAHSLFKSVKHLSAGCRTLADRCV
                     RGISANVQRMHDTVTHSIGIVTALNPYIGYAHATEVAQAAHASGGSVYDEVLKRGLMT
                     REQLDAVLRPETLTRPVPLDLAPKRS"
     gene            7059..8036
                     /locus_tag="PK242_07945"
     CDS             7059..8036
                     /locus_tag="PK242_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOZ93987.1"
                     /translation="MVTRKPKGLGRGLDALLGPSPNGDGAEGAGAATPAGTPMMLALE
                     QLAPGQYQPRTRMDEGALYELAESIKSQGIMQPILVRRIAGPKPASGLDGQAPAATFS
                     GASYEIIAGERRFRAARLAGLTEVPVLLRDVPDEAAAAMALIENIQREDLNPLEEAQG
                     IRRLVEEFGLTHEQAAQAVGKSRSAASNLLRLLQLSEPVQTMLMAGDLDMGHARALLA
                     LEGAAQITAAHQVAARQLSVRETEALARKLSAEFNLTAPRQRARAPDKSGDVRRVEEE
                     LSDLLLAQVEVRVRKSGRRGGAAAQAGEVVIQFASLDELNGLIERLRGG"
     gene            8141..9448
                     /locus_tag="PK242_07950"
     CDS             8141..9448
                     /locus_tag="PK242_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93988.1"
                     /translation="MSLVPSLVRHVLLLAALGGGVAGVASAQAARAPRAQLWIDLSTG
                     GMAGMPEMELGAAGGLMGMLGSSAPAGMGGPPHYGMARGAAVMPPRIVDIALHNSLRP
                     GVEARQAIPPGMRMGDSLPLLPPRAEPRAPSEPGDMPEEYTRQPPRGRLLIYWGCGPE
                     VRAGQPRVIDLAQAGAAQLAQAFAGRVVPERGARVGPGHVLYPNERSQAAVPRGSTLV
                     GEHQVLGEGVPASMKFTLGSAQDLMPPIELSPSGGVQDSIATQWRAVPHARAYYLHAM
                     ASAGDDMILWSSAETPDTGMGLFDYLPNATIERWVRERVLLGADVTQCAIPRGIFAAG
                     GSDATPMLRMMAFGGESHIVHPPRPADPKARWEPEWAVRVRVKAHTMAMLGDSGAAMR
                     GARPGGGASAGATGQGAEPRPDDGSPAQILLNPGNLLRGIFGR"
     gene            9610..10230
                     /locus_tag="PK242_07955"
     CDS             9610..10230
                     /locus_tag="PK242_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ93989.1"
                     /translation="MRRAVEQVVNGSSHSLFLRALVAQPARVGAICASSPQLAARMAA
                     WVDPDAPGLVVELGGGTGVITAALLARGVAPQRLVVIEQSPVLARHLAQRFPQVRVVQ
                     GDAAELALLQRAPDWPRGDDGAPRPVHCIVSGLPLLSIPPQLRQRILQAGAQTLAPGG
                     RLLQFTYALRGPSPWQVTGLSGCARERVLANLPPARIDVLQRPHLG"
BASE COUNT         1454 a         3754 c         3708 g         1456 t
ORIGIN      
        1 cgctggccac cgacgaggcc ggcattcagt ttcgcattct caacagcagc aagggcccgg
       61 cggtgcgcgc cacgcgggcg caggccgacc gtgtgcgcta caaggcggcg atccggcgcc
      121 ggctggaaaa ccagcccaac ctgtggctgt tccagcaggc ggtggacgac ctgctgctgc
      181 agggcgatcg ggtggccggc gcggtgaccc agctgggcat cgccttccgt gcgcgcgccg
      241 tggtgctgac ggcgggtacg ttcctggacg ggcgcatcca cgtcggtctg gccaaccacg
      301 ccggcgggcg ggcgggcgac ccgccggcgc tcagcctgtc ggcgcggctg aaggagctca
      361 agctgccgca ggggcgcctg aagaccggca cgccgccgcg gctggacggg cgcacgatcg
      421 acttcagcca gtgcgagctg caacccggcg acggcatgcc gggcgggcag aacgaccgca
      481 tgccggtgtt cagcttcatg ggccgcgccg acatgcaccc gccgcagatg ccgtgctggg
      541 tcacgcacac caatgcgcgc acgcacgaga tcatcaagag cggcttcgac cgcagcccga
      601 tgttcaccgg cgtcatcgaa ggggtggggc cgcgctactg ccccagcgtg gaagacaagg
      661 tccaccgctt tgccggcaag gacagccacc agatcttcct ggagccggaa gggctggaca
      721 cgcacgaggt ctaccccaac ggcatctcca ccagcctgcc gttcgacgtg cagctggcgc
      781 tggtgcgcag catgcgcggg ctggaaaacg cccacatcct gcgtcccggc tacgccatcg
      841 aatacgacta tttcgatccg cgcgcgctgc gcagcagctt cgagacgcgc gccatcggcg
      901 gcctgttctt cgccggccag atcaacggca ccaccggcta cgaggaggcg gcggcgcagg
      961 gactgtttgc cggcatcaac gccgccctgc agtgccgggg cgaggcgccc tggctgccgc
     1021 gccgcgacga ggcctacctg ggcgtgctgg tggacgatct ggtgacgcag ggcgtgaccg
     1081 agccttaccg catgttcacc agccgcgccg aattccgcct gcaactgcgc gaggacaacg
     1141 ccgacatgcg cctgaccgac accggccgcc ggctgggcct gatcgacgac gcgcgctggg
     1201 acgccttctg ccgcaagcgc gacgcggttt cacgtgaaac cgaacggctg aagtccacct
     1261 gggtcaaccc gcgccgactg cccgccagcg agagcgagcg cgtgctgggc aaggccatcg
     1321 agcatgagca caggctgttc gacctgctgc gtcgccccgg tgtcagtcac gaagcgctgg
     1381 ccggactgga tggcggcaaa tacgccagcg ccgaggtttc acgtgaaacg ttgggcgagc
     1441 tgtgggcgcc ggtgatcgag caggtggaaa ttgccgccaa gtacgccggc tacatcgacc
     1501 gccagcgcga ggaggtcgag cgcgctgccc atttcgagca cctgcgcctg ccgcccgatc
     1561 tggactacat gcaggtgccg gcgctgtcga tcgaagtgcg ccagaagctc aacctgcacc
     1621 ggcccgagac cctgggtcag gccagccgca tcagcggggt gacgccggcc gccatttcgc
     1681 tgctgctgat ccacctgaaa aagggcggtt tcaagggctt tgccggcggc ggcgcggccg
     1741 aggccgcctg atgcacccgc aacgtgcggc gctcggcgcc ggcgcgcagg cgctcggcct
     1801 ggcgctggat gagcggcaga tcgatcgcct gctggcctac ctcgatctgc tgctcaagtg
     1861 gaaccgggtc tacaacctga cggcgctgcg cgaccccgcc gacatgctga cccatcatgt
     1921 gctggacagc ctggcgctga tcgcgccgct gcgggcgcac gccggcgggc gggcgctgcg
     1981 cctgctcgac gtgggcagcg gcggcggcct gccgggggtg gtgattgcca tcggctgccc
     2041 cggcatcgac gtcacctgtg tcgatgccgt gggcaagaag gccgccttcg tgcagcaggt
     2101 ggcgggcgcg ctggggctgg ccaacctgca cgggcagcac gggcgggtcg aggccatcac
     2161 gggccgattc gatgtggtga cgtcgcgcgc cttcgcctcg ctggccgatt tcaccgcctg
     2221 gtcggacggc gcgctggccg agggcggggt gtggctggcc atgaagggcc gccggccgga
     2281 cgacgaaatc gccgccttgc cgcccgcggt gaccgtgttt cacgtggaac cgctcacggt
     2341 tcccggcctg gaggctgagc gctgcctcgt ctggctgcgc cgcgccgggt aaactcgcgg
     2401 cgcactccag gatcattccc atgttcggca tttccgatta cggtgccttt gtggccgcca
     2461 tcgtgctgtt tctggccgtc cccgggccgg gcaacctggc cctgctgtcc agcacggcgc
     2521 agggtggcgt ccgcggcggg ctggcggcca cgctggggtt gattgccggc gaccaggtgc
     2581 tgctgtggtg cgccgtcgcc ggcgtcgcgg cgctgctgca gagcgcgccg accctgttcg
     2641 ccgccgtgca gtggctgggc gcggcctacc tggcctggct gggctggcgc atgctgagcg
     2701 cccggccggg gcagggcgcg gtgctgcgcc tggcgccacg ccactttctg cgccaggcgt
     2761 ttctgatcac cttgctcaac cccaaggcca tcgtgttcta catggccttc tttccgctgt
     2821 tcgtcgatcc ggcgcggcac caggggctgc tgagcttcgg cgccatggca ctgacgatcg
     2881 ccgtgctgac cttcctgtac ggcctcgggg ccacgctgat cgctcaccac atggcggcgc
     2941 gcatccgcgc ccacccgcgc ctgtcgggcg ggctgcagaa gctggccggg gtgttcctga
     3001 tcggcttcgg cctgcgcctg gcgctcacca agtaagacct catggcccac atcttctgca
     3061 ttgccaacca gaagggtggc gttggcaaga ccaccaccac cgtcaacctg gccgccgggc
     3121 tggccaggat cggccagcgc gtgctgctgg tcgacctgga cccgcagggc aatgccacca
     3181 tggggtcggg cgtggacaaa cgggccatgg cgctgtcggt gtacgacgtg ctgctggaat
     3241 cctgcagcat cgccgaagcc gccagcgtgc ccgagcgcct gaaggaggcc ggtgccggct
     3301 acgccgtgct gggcgccaac cgcgaactgg ccggcgccga ggtcgagctg gtgggcgagg
     3361 agcgccgcga gcgccgcctg aagcaggcgc tggcgcaggt cgacgccgac tacgacttcg
     3421 tgctgatcga ctgcccgccc agcctgagca tgcttaccct gaacggcctg tgcgccgccc
     3481 agggcgtgat cgtgcccatg cagtgcgaat acttcgcgct cgaaggcctg gccgacctga
     3541 ccaacaccat ccgccaggtg cacgccaacc tgaaccgcga cctgcgcctg atcggtctgc
     3601 tgcgtgtcat gttcgacgcc cgcatcacgc tgcagcagca ggtcagcgac cagctcaagg
     3661 cgcattacgg cagcaaggtg ttcgacacca tcatcccgcg caacgtgcgc ctggccgagg
     3721 cgcccagcta cggcctgcct ggcgtggtgt tcgacccggc ggccaagggc agccaggcgt
     3781 tcgtcgcctt cgcggaagag ctggtgcgcc gtggtttgag tgcctgaaag gcctctggcg
     3841 cccgtccaga ttccgccggc agctatagaa attgaagtca ctgactgaac cccgcacgcc
     3901 gccggccgcc ggcctgcccg aaccctggat cgactggggc aaggccatcg cctcgcagct
     3961 catcgtctgg caccacctgg cgcactacgg tccgctggcg ccgcgcgtgg ccgccgtctg
     4021 gccggacctg atgggctggc tggacgggcg cgccctgtac gcggtgcagg cgtttctggt
     4081 catgggcggc tatctggccg cgcgcagcct gtggccacgg cccggcgcgc cgcgcgtggc
     4141 ggcggccgac tggccggccc gcgcggcccg gcgctggcgg cgcctgatgc cgacctacct
     4201 ggcggcgctg ctgctggccg tgctgtgcgc ggcgctggcg cgccagtgga tggacgaccc
     4261 cgataccccg gccgccccca cggcggggca gttgctggcg catctgctgc tgctgcagga
     4321 cctgctggag cagccggccc tgagcgccgg cctgtggtat gtggccatcg atctgcagct
     4381 gtacgccctg ctggcggcgc tggcgctgct ggccggccgg gccccggcgc gctggcgccg
     4441 cgcctcggcg ctggccctgg tggtcggcgg cggcgcggcc tcggtgctgg tgttcaaccg
     4501 ccagcccggc ggcgacgagc tggcgccgta cttctttggc agctacgccc tgggcgtgct
     4561 ggccgcctgg ggccgtgcgg ccggcacggc ggcggggcgc cgcgccgccg gggcgctgct
     4621 gctggggctg gccggcgccg gcctggcgct ggcctggcgc gaccgcctgg cgctggccgc
     4681 gctctgcgcc atgctgctgc tgtggcagcc cgggcgcacg cgtctggcac tggccggcca
     4741 gccctggctg cgcagcgtcg tggcgcagct ggcggccatc tcctacgccg tgtttctgct
     4801 gcactacccg atcagcctgc tggtcaccgc cctgctgatc gacgcgctgc cggccggccc
     4861 ggtgccggcc gccgcgctgc tggagctgac ctggctgctc agcctggcga ccggctgggc
     4921 gctgacgcag gcgctgcaag cccccggcct gtggcagcgg ctgcgccgtc tcaccgcgcg
     4981 gtggcgccat ggcacacgct gacgcacccg ccgccaccgt gctgaccctg ccgggctggc
     5041 tgggcagcgg gccggcgcac tggcaaagcc tctgggagca gcgccacggc tgccagcgcg
     5101 tcgagcagca cgactggcag cacccgcggc gcggcgactg gtcggcgcgg ctggaagagg
     5161 tggtgctggc gcagccggcc ggcacgccgc tgctgctggc cgcgcacagc ctgggctgcc
     5221 tgctggtggc ctggtgggcc gcgcattcgc gcctcgccac gcgcgtgagc ggcgcgctgc
     5281 tggtggcgcc gcccgagatc gagcgcgacg acctgcgcca gcagatcccc ggctgggcgc
     5341 cgccggcgcg ccagcgcttg ccctttgcca gcctggtggt ggccagcagc gacgacccgt
     5401 ttggccgact ggcccatgcc cgggcgctgg cgcaggactg gggcgccgcc ttccgcgatc
     5461 tgggcgcgcg cggccacctc aacgccgatt ccggcctggg tgactgggcg cagggcctgg
     5521 cgctgctgta cgccttgttg ccatcgccca cctgtgcccc gggcgggtca accgcttaga
     5581 ctggcgtcca tgagcacccc caaccccaac cgcatcgagc acgacctgat cggcgagcgc
     5641 gagctgccca acgacgtcta ctacggcgtg cacaccctgc gcgcgctgga gaactttccg
     5701 atcacgggca cggcgatctc gatctacccc gacctggtcc gcgcgctggc cagcatcaag
     5761 aaggccgcgg cgctggccaa ccgcgacctc ggcctgctca gcgacgcgca ctgccaggcc
     5821 atcggcgccg cctgcgacga gctgctggcc ggcaagctgc acgagcagtt cgtggtcgac
     5881 gtcatccagg gcggcgccgg cacctcgacc aacatgaacg ccaacgaggt catcgccaac
     5941 cgcgcgctcg agctgatggg ccacgccaag ggcgagttcc agcacctgca ccccaacgag
     6001 cacgtcaacc tgggccagag caccaacgac gtctacccca gcgcgctcaa gatcgccgcc
     6061 tggcacggca tcagcgacct gatcgcatcc atggcctacc tgcgcgccgc cttcgaggcc
     6121 aaggccgagg aattcaagga cgtgctcaag atgggccgca cccagctgca ggacgcggtg
     6181 ccgatgacgc tggggcagga gttcggcacc tacgccgtca tgctgggcga ggacgagcag
     6241 cgcctgcgcg aggcgcagca actgatctgt gaactgaaca tgggggcgac ggccatcggc
     6301 accggcatca ccgcccaccc cgactatgcc gccaaggtga tcgcgcacct gcgcgccatc
     6361 accggcatcc cgctggccac cgcgcccaac ctgatcgagg cgacgcagga ctgcggcgct
     6421 ttcgtgcagc tgtcgggcgt cttgaagcgc gtggccacca agctgtccaa gacctgccac
     6481 gacctgcgcc tgctgtccag cggcccgcgc gccggcctgg gcgagatcaa cctgccggcg
     6541 gtgcaggccg gctcgtccat catgccgggc aaggtcaacc cggtgatccc cgaagtggtc
     6601 aaccagatcg cctacgaggt gatcggcaac gacgtgacgg tcaccttcgc cgccgaggcc
     6661 gggcagctgc agctcaacgc cttcgagccg gtgatcgcgc acagcctgtt caagagcgtc
     6721 aaacacctgt cggccggctg ccgcacgctg gccgaccgct gcgtgcgcgg catcagcgcc
     6781 aacgtgcagc gcatgcacga cacggtgacg cactcgatcg gcatcgtcac cgcgctcaac
     6841 ccctacatcg gctacgccca cgccaccgaa gtggcgcagg ccgcacatgc cagcggcggc
     6901 agcgtgtacg acgaagtcct caagcgcggc ctgatgacgc gcgagcagct cgacgccgtg
     6961 ctgcggcccg aaaccctgac ccgcccggtg ccgctcgacc tggcgcccaa gcggtcgtga
     7021 tccggtcgac cgcgcaccgt acgggagatc gatcgaccat ggtcaccaga aaacccaagg
     7081 gcctcggccg cggcctggac gcgctgctgg gccccagccc gaacggcgac ggcgccgagg
     7141 gcgccggcgc cgccaccccg gccggcacgc cgatgatgct ggcgctggag cagctcgctc
     7201 ccggccagta ccagccgcgc acgcgcatgg acgaaggcgc gctctacgag ctggccgaaa
     7261 gcatcaagag ccagggcatc atgcagccca tcctggtgcg ccgcattgcc gggccaaaac
     7321 cggcctcggg gcttgatgga caagcgccag cagctacatt ttcgggagca tcgtacgaaa
     7381 tcatcgccgg cgagcggcgc tttcgcgcgg cgcgtctggc gggcctgacc gaggtgccgg
     7441 tgctgttgcg cgacgtgccc gacgaggccg ccgccgccat ggcgctgatc gaaaacattc
     7501 agcgcgaaga cctcaacccg ctggaagaag cccagggcat ccggcgcctg gtcgaggagt
     7561 tcggcctcac ccacgagcag gccgcacagg cggtcggcaa gagccgcagc gccgccagca
     7621 acctgctgcg cctgctgcag ctttccgaac cggtgcagac catgctgatg gccggcgacc
     7681 tggacatggg ccacgcgcgc gcgctgctgg cgctggaagg cgcggcgcag atcaccgcgg
     7741 cccaccaggt ggcggcgcgc cagctcagcg tgcgcgaaac cgaggcgctg gcgcgcaagc
     7801 tgtcggccga attcaacctg acggcgccgc gccagcgcgc gcgcgcgccc gacaagtcgg
     7861 gcgacgtgcg gcgggtggag gaggagctgt ccgacctgct gctggcgcag gtcgaggtgc
     7921 gggtcagaaa aagcggccgg cgcggcggcg cggcggccca ggcgggcgaa gtggtgatcc
     7981 agttcgcgtc gctggacgag ctcaacgggc tgatcgagcg gctgcgcggc ggatgatgca
     8041 ggggcccgaa cgcgctgcac ggcgcccgat cggcatacac tgaaccatcg tccgcacggc
     8101 gtgtgcgcca catcaccgaa acgaacgcag gaatcccatc atgagcctgg tgccatcgct
     8161 cgtccgacat gtgctgctgc tggccgccct gggtgggggt gtggcgggcg tcgcctcggc
     8221 gcaggccgcg cgcgcgccca gggcgcagct gtggatcgac ctgtccaccg gcggcatggc
     8281 cggcatgccc gagatggagc tgggcgcggc cggcggcctg atgggcatgc tgggcagcag
     8341 cgcacccgcc ggcatgggcg ggccgccgca ctacggcatg gcgcgcggtg cggcggtgat
     8401 gccgccgcgc atcgtcgaca tcgcgctgca caacagcctg cgccccggtg tcgaggcacg
     8461 ccaggccatc ccgcccggca tgcgcatggg cgacagcctg ccgctgctgc cgccgcgtgc
     8521 cgaaccccgc gcgccctccg agccgggcga catgcccgag gagtacacgc gccagccgcc
     8581 gcgcgggcgc ctgctgatct actggggttg cgggcccgag gtgcgcgccg gccagccgcg
     8641 cgtgatcgat ctggcccagg ccggcgcggc gcagctggcg caggcctttg ccgggcgcgt
     8701 ggtgcccgaa cgcggcgcgc gcgtcggccc gggccatgtg ctgtatccca acgaacgcag
     8761 ccaggcggcg gtgccgcgcg gcagcacgct ggtgggcgaa caccaggtgc tgggcgaagg
     8821 ggtgccggcg tcgatgaagt tcactctggg cagcgcccag gacctgatgc cgccgatcga
     8881 gctcagcccc agcggcggcg tgcaggacag catcgccacc caatggcgcg ccgtgcccca
     8941 tgcgcgcgcc tactatctgc acgccatggc gtcggccggc gacgacatga tcctgtggtc
     9001 cagcgccgaa acgccggaca ccggcatggg cctgttcgac tacctgccca acgccaccat
     9061 cgagcgctgg gtgcgtgagc gcgtgctgct gggggccgac gtcacccagt gcgccattcc
     9121 gcgcggcatc ttcgccgccg gcggcagcga cgccaccccc atgctgcgca tgatggcctt
     9181 cgggggcgag agccacatcg tgcacccgcc ccgtccggcc gacccgaagg cgcgctggga
     9241 gcccgaatgg gcggtgcgcg tgcgcgtcaa ggcccacacc atggccatgc tgggcgacag
     9301 cggtgccgcc atgcgcggtg cccggccggg cggcggcgcc agcgccggtg ccaccgggca
     9361 aggcgccgag ccccggccgg acgacggctc gccggcgcag atcctgctca acccgggcaa
     9421 cctgctgcgc ggcatcttcg ggcgttgaac ggtcgaacca tgagcgtgtc cgatccttct
     9481 tcgtccggcg ctgccgacgt gccgcagcgc cctggcgtgc gcgccgtggt ccggctggcg
     9541 cgccgccgtg gtccggccag ggcggcaccg ctgctgaccc tgcgggccgc gcgcgccggc
     9601 gtactgcgca tgcgccgggc cgtagagcag gtcgtcaacg gctcgtcgca ctcgctgttc
     9661 ctgcgcgccc tggtggcgca gccggcccgg gtgggggcca tctgcgccag ctcgccccag
     9721 ctggcggcac gcatggcggc ctgggtcgat cccgacgcgc cgggcctggt ggtcgagctg
     9781 ggcggtggca ccggcgtcat caccgccgcc ctgctggcgc gcggtgtggc gcctcaacgt
     9841 ctggtggtga tcgaacagtc gccggtgctg gcgcggcacc tggcgcagcg cttcccgcag
     9901 gtgcgggtgg tgcagggcga tgccgccgag ctggccctgc tgcagcgcgc acccgactgg
     9961 ccgcgcggcg acgatggcgc gccccggccg gtgcactgca tcgtgtccgg cctgccgctg
    10021 ctgtcgattc cgccgcagct gcgtcagcgc atcctgcagg ccggggcgca gacgctggcg
    10081 ccgggcgggc gcctgctgca gttcacctat gcgctgcggg ggccttcgcc ctggcaggtg
    10141 acgggcctgt cgggctgtgc gcgtgagcga gtgctggcca acctgccgcc tgcgcggatc
    10201 gacgtgctgc aacgaccgca cctcggctga gggcactgat ccggcaccat ttctgggtct
    10261 ggggcccgcc cataaagcgc taatagctat taaacctaga aacggcagtt ggacgcgccc
    10321 gcccgtgccg tgatcggcgt gccaggcaag acgcgacgac gccgcgggct cc
//
LOCUS       DAOOOG010000090        10236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_7484_length_10236_cov_27.445536, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000090 DAOOOG010000000
VERSION     DAOOOG010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7484_length_10236_cov_27.445536"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PK242_07960"
     CDS             <1..503
                     /locus_tag="PK242_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ93990.1"
                     /translation="AFVDQSREALADDKTVWEDVSGGLDLITVGKFQMPSRAYCGRFN
                     FNGQDQQKKVGMLSGGERGRLHLAKTLMQGGNVLLLDEPSNDLDVETLRALEDALLEF
                     AGSVMVISHDRWFLDRICTHILAAEGDSQWVFFDGNYQEYEADKIKRLGEEGARPHRV
                     RYKALK"
     gene            535..1191
                     /locus_tag="PK242_07965"
     CDS             535..1191
                     /locus_tag="PK242_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93991.1"
                     /translation="MGTPATLIVTGFDAQHFDLAQDFADSFRQAYQEQYQLAAVVFGP
                     QAPPPQLSDRFDRITRLDASGPSFDAGRNGYFLAHAGVNARLRDIFPGYASYCWVDAD
                     CWFQGDESLPRILAGMPGFDLCIHPEFDVHYLNFPTPSERTLHIYRTNEAANLSAMPL
                     RMPMVNSGVFAMRTDSRVWALWDAELTGLRQRHERGEAVFFSDQIALHKLVYLNQLRI
                     "
     gene            1267..1398
                     /locus_tag="PK242_07970"
     CDS             1267..1398
                     /locus_tag="PK242_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93992.1"
                     /translation="MPTPPHEKIGVMHLAGRSKHQQLQIDGQTLTLRYRDMARLFAA"
     gene            complement(1493..1681)
                     /locus_tag="PK242_07975"
     CDS             complement(1493..1681)
                     /locus_tag="PK242_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOZ93993.1"
                     /translation="MKNVGGIDRILRIVVGLVLVGLAATGSVGWWGWIGVVPLLTGLL
                     GSCPAYKLLGMNTCPMKK"
     gene            complement(1879..2226)
                     /locus_tag="PK242_07980"
     CDS             complement(1879..2226)
                     /locus_tag="PK242_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ93994.1"
                     /translation="MYEIWLMLNIVYELALSIWPWLLALAALWLLLMGRAWGRRGADW
                     RASLPGAIVLGLLVAIVIFAVTPVWNRSALGEMKYWVDWANLLGIAAAWGVAGVAFGW
                     PLLTGLRRSGKVG"
     gene            complement(2254..3588)
                     /locus_tag="PK242_07985"
     CDS             complement(2254..3588)
                     /locus_tag="PK242_07985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOZ93995.1"
                     /translation="MTHRSDQRPSRRDLLRTLGAAPLAASALQAAPTARAQTSSSQAR
                     IVIAGSGLGGIAVASRLRQLVPGAKITIVDAKQEHNYQPGYTLVATGIWPIEKVKNRN
                     AEFQPEGVEWVQEMVASFDPASNSLTTTGGRKIGYDFLVVATGLQLGYDQIEGMDVKA
                     IGSNGLGSVYASPEAALATWQAMDAFRAKGGQAVMTLPAGPLKCAGAPLKMTFMVADR
                     LRQAGTLDKSKVDFFSGLPDDTVFGVKSVNDNVLARWKALGIQTHYLRKLVAIDPGAR
                     KAFFTSPEGERQEVGYDFLHVVPPMRAPDAVKGSDLAAKDGPMAAGGWLEVSKDTLQH
                     KRYPNVFGVGDINGTPRGKTAATVKKSAPLVAHNLVRVIDGQPPDQIFDGYTSCPMIV
                     REGSAMLIEFDYDGKLTPSLPMIEPLQESFMAWFMKYRLLKPAYIAVLKGRV"
     gene            complement(3616..4320)
                     /locus_tag="PK242_07990"
     CDS             complement(3616..4320)
                     /locus_tag="PK242_07990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ93996.1"
                     /translation="MDWSQARIERDELNLLAELVPLAGLQVIELGCGSARLARDLLAR
                     HPDARVTGLEVDAIQHARNLAAPAERLSFIAAGAEAIPLPEAQFDLALMLKSLHHVPL
                     ALMDQALREVHRVLRTGGHLYVSEPVYAGALNQITRLYNDEGAVRAAAQAALDRALAS
                     GQWEAAAERHFAVPVRYADFADFERRMLDVTYAERHLDDDLREQVRARFAAHVGPEGA
                     HFQRPMHVRLLRKAGA"
     gene            complement(4332..5474)
                     /locus_tag="PK242_07995"
     CDS             complement(4332..5474)
                     /locus_tag="PK242_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ93997.1"
                     /translation="MNGVAAPQIVVLGAGFGALSTVRSLRQQGCTAPITLVAPRAELH
                     YLPGIIWIPSGLRTRADLVVPLDNFFARMNVRHHLASVTGLSDDGRVVHTSAGELANE
                     ALVIASGARFIRKLPGIEHAITPCEGIDAAERIRDRLRQMSGGTIAVGFGANPNEPSA
                     VRGGPMFEFLFGIDRQLRREGRRERFQLVFFNPSQQPGARLGSRTVARLLAMMAQRGI
                     QTHLGHKMVRFEADRVVTEGGEFAADLILFMPGLTGPAWLEQSSLPRSPGGLIAADAG
                     CRVPQLERVYVVGDSGSFPGPDWMPKQAHMADLQAEAAARNLLSELAGQPGTQGFKVE
                     LLCIIDALEEGTLVLRTERRQIVLPPTRLMHWAKRSFERRYLARYR"
     gene            complement(5471..6844)
                     /locus_tag="PK242_08000"
     CDS             complement(5471..6844)
                     /locus_tag="PK242_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOZ93998.1"
                     /translation="MKRLTRLVLVGAALGVPVGAWAQDLLTVWRAAAGHDQRLAVARA
                     EHGASQTLRAQADALWRPQVGLNLGLGLGVQDTAMRGARFSAPGMGQVDEARFATSVT
                     GGLATRVAVQAQQPLVNATRQAQQAQLRLGADMGDIAWRGAQADLMLRTTERYLALAV
                     AQERVRVVERQWQAVSRSRAEAHDRYQLGASPITDTHEADAALAGVRAQQSAAELELS
                     VQRDRLAGSTGLAQPRARLPLAPLAPVEALPFWLAAAESDNPRLRLLAQAVALAEQKL
                     RQHSVAGRATLDLVAQAGHERIAGGGDFGSARNRSLNGMVGVQLNIPLYTGGMREAQE
                     REAAERLNQAMAELELAREDVAQQVRATWQGLQTGAARLAALQEGLVASRARLDATRL
                     GREVGDRTTLDVLNAENAQADAELALAQARSDQVLTRLRLAALADRLDEALLTQVDAT
                     LAPEVPR"
     gene            complement(6841..7197)
                     /locus_tag="PK242_08005"
     CDS             complement(6841..7197)
                     /locus_tag="PK242_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOZ93999.1"
                     /translation="MVENYTNLIAAVSANSAKLRKDIPEVMQGFNAMAQAASKAGALD
                     AKTKELIAMALSVGARCDPCVGYHAKALVKLGATRAEVEEMLGMCVYMGGGPGLMYAA
                     SALAAYEEFGGEKASA"
     gene            complement(7229..7444)
                     /locus_tag="PK242_08010"
     CDS             complement(7229..7444)
                     /locus_tag="PK242_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOZ94000.1"
                     /translation="MTSWRIVRIFAGTFILLSLALGVQGSPIFHSSNWLWFTAFVGAN
                     LLQSGFTKWCLLNNILLKLGVPAESCS"
     gene            complement(7460..>10236)
                     /locus_tag="PK242_08015"
     CDS             complement(7460..>10236)
                     /locus_tag="PK242_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ94001.1"
                     /translation="SLESELKRVPGTREVYTLGGPGRAVMVEADPQRLAAAGVSLHEL
                     HGALQSANLGLPVGELLGGNRALAVETGAYLHSAQDVAELVLAVRGGKPVYLREVAQV
                     RDGAPPPTRYVWHGEVRRGDGGAPATALEQPAVTLAITKKPGENAIDVADAVLRRVDQ
                     LRNTVLPADVQVTETRNYGATANDKATTLIKKLLFATLSVVALVFFALGRREAAIVGV
                     AVILTLTVTLFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRHQSLYPERTL
                     AQIIPGAVDEVGGPTILATFTVIAALLPMAFVSGLMGPYMSPIPINASMGMLLSLAIA
                     FSVTPWLARLWMKATHQADHGQAAGHGLAARLTPLFERIFRPLLDERRGARHRRLMGL
                     AVAGLIGLSVALPVLGLVVLKMLPFDNKSEFQVVVDMPAGTPVEKTAAVLRELGAHLA
                     SVPEVTHYQAYAGTAAPINFNGLVRQYYLRAGGEVGDLQVNLVDKAQRKDQSHAIAVR
                     ERAALRAIGQRHGANVKVVEVPPGPPVLAPIVAEIYGPDADGRRAVAQQVRAVFEATP
                     GVVDVDDTTIVDAPRTVLVVDRRKAAQLGVSQQAIASTLRMGLTGDAATYLHDASRFA
                     VPAFIQLPAELHGSLDALLQLGVRSASGALVPIRELVTPSNTLREQPIYHKDLLPVSF
                     VMGDMAGQRAGELDSPLYGMFQMRGALAELKMPDGGRLDEYFIRQPQDPYRGYALKWD
                     GEWQITYETFRDMGAAYAVGLVLIYLLVVAHFGSYLTPLIIMAPIPLTIIGVMPGHAL
                     LGAQFTATSMIGMIALAGIIVRNSILLVDFIKLQVSEGMPLARAIVQSAIIRAQPIGL
                     TALAAMIGALFILDDPIFNGLAISLIFGILVSTVLTLVVIPILYFAAYRRTHEKNSAL
                     APTQPAQAAT"
BASE COUNT         1600 a         3584 c         3493 g         1559 t
ORIGIN      
        1 tggccttcgt cgaccagagc cgcgaggcgc tggccgacga caagacggtg tgggaagacg
       61 tctccggcgg cctggacctg atcaccgtcg gcaagttcca gatgcccagc cgggcctact
      121 gcgggcgctt caacttcaac ggccaggacc agcagaagaa ggtcggcatg ctgtcgggcg
      181 gcgaacgcgg ccgcctgcac ctggccaaga cgctgatgca gggcggcaac gtgctgctgc
      241 tggacgaacc gtcgaacgac ctcgacgtcg aaaccctgcg cgcgctggaa gacgcgctgc
      301 tcgaattcgc cggcagcgtg atggtgatca gccacgaccg ctggttcctc gaccgcatct
      361 gcacccacat cctggccgcc gagggcgaca gccagtgggt gttcttcgac ggcaactacc
      421 aggagtacga agccgacaag atcaagcgcc tgggcgaaga aggcgcgcgg ccacaccggg
      481 tgcgctacaa ggccctcaag tgaggccagg ccgcaagacg ctgcaggaaa gccgatgggc
      541 acccccgcca ccctgatcgt caccggcttc gacgcgcagc atttcgatct ggcgcaggat
      601 tttgccgact cgtttcgcca agcctaccag gaacagtacc agctggccgc ggtcgtgttc
      661 ggccctcagg cgccgccgcc ccagttgtct gaccggttcg atcggatcac ccggcttgac
      721 gccagtggcc cgtcgttcga cgctggaagg aacgggtact ttcttgccca tgccggagtg
      781 aacgcccgac tgcgcgacat ctttccgggc tatgccagct actgctgggt cgacgccgac
      841 tgctggtttc agggcgatga atccctgcca cgcatcctgg ccggcatgcc ggggttcgac
      901 ctctgcatcc accccgagtt cgacgtccac tacctgaact tccccacgcc gagcgaacgc
      961 acgctccaca tctatcggac caatgaagcg gccaacctgt cggccatgcc gctgcgcatg
     1021 cccatggtca attccggcgt ttttgccatg cggaccgact ctcgtgtgtg ggccctgtgg
     1081 gatgccgaac tgaccgggct gcgccagcgg cacgagcgcg gcgaagcggt attcttttcc
     1141 gaccagattg cgctgcacaa gctggtctac ctgaaccagt tgcgcatcta accgctgcgt
     1201 gccatcgaca actggcagac ctacgcctgc ctgccgatga ttcaccggga gacacggtgt
     1261 ctgcgcgtgc ccacaccgcc gcacgagaaa atcggcgtca tgcacctggc cggccgcagc
     1321 aaacaccagc agctgcagat cgacgggcag acgctgaccc tgcgctaccg cgacatggcc
     1381 aggctgttcg ccgcctgacg ccgttcgtca ggccatatcc gtcagaaacc cttaccgaca
     1441 gtgcgccagc agctatcgat agtgaagcat gcgggcctct gtcgggacgg gttcacttct
     1501 tcatcggaca ggtgttcatg cccagcagct tgtaggccgg gcaactgccc agcaggccgg
     1561 tgagcagcgg caccacgccg atccagcccc accagcccac gctgccggtg gccgccaggc
     1621 ccaccagcac caggccgacg acgatgcgaa gaatacgatc gataccaccc acgtttttca
     1681 tgacagactc cttgttgcag tgttgaatgt ttgagcgcag aaggcaggaa ggcaggcccc
     1741 atgcccgaag cgggcgcccc cacgccgccg atccaggcgc caggcacggc cgcagcctgg
     1801 gctgcggcga gcatggtcaa cgccagaccg gcggttcggt gcgggccagg cgggcatgag
     1861 ggattcgttc acaccttgtc agcccacttt gccagaacgg cgcagcccgg tcagcagcgg
     1921 ccagccaaag gccacgccgg caacgcccca ggcggcggcg atgcccagca ggttggccca
     1981 gtcgacccag tatttcatct cgcccagtgc agacctgttc cagaccgggg tgacggcgaa
     2041 gatgacgatg gccaccagca ggcccagcac gatggcgccg ggcaggctgg cgcgccagtc
     2101 ggcaccccgg cgaccccagg cgcgccccat cagcagcagc cacagggcgg ccagcgccag
     2161 cagccagggc cagatcgaca gggccagttc gtagacgatg ttcagcatca accagatttc
     2221 atacatgacg gctctccttc ggtcacggga tggtcagacg cggcccttga gcaccgcgat
     2281 gtaggccggc ttgagcaggc ggtacttcat gaaccaggcc atgaagctct cctgcagcgg
     2341 ctcgatcatg ggcagcgagg gcgtgagctt gccgtcgtag tcgaactcga tcagcatggc
     2401 cgagccttcg cgcacgatca tcgggcagga ggtgtaaccg tcgaagatct ggtcgggcgg
     2461 ctgcccgtcg atcacgcgca ccaggttgtg cgccaccagc ggcgcgctct tcttgaccgt
     2521 ggccgcggtc ttgccgcgcg gcgtgccgtt gatgtcgccg acgccgaaga cgttgggata
     2581 gcgcttgtgc tgcagcgtgt ccttgctgac ttccagccag ccgccggcgg ccatcgggcc
     2641 gtccttggcg gccagatcgc tgcccttgac ggcgtcgggc gcgcgcatgg gcggcaccac
     2701 gtgcaggaag tcgtagccga cctcctggcg ctcgccctcg gggctggtga agaaggcctt
     2761 gcgcgccccc ggatcgatcg ccaccagctt gcgcaggtaa tgcgtctgga tgcccagcgc
     2821 cttccagcgc gccagcacgt tgtcgttgac cgacttgacg ccgaacacgg tgtcgtcggg
     2881 caggccgctg aagaagtcga ccttggactt gtccagcgtg ccggcctggc gcaggcgatc
     2941 ggccaccatg aaggtcatct tcagcggcgc accggcgcat ttgagcggcc cggcgggcag
     3001 cgtcatcacc gcctggccgc ccttggcgcg gaaggcgtcc atggcctgcc aggtggccag
     3061 cgcggcctcg gggctggcgt agacgctgcc caggccgttg ctgccgatgg ccttcacgtc
     3121 catgccctcg atctggtcgt aacccagttg caggccggtg gccaccacca ggaagtcgta
     3181 gccgatcttg cggccgccgg tggtggtcag gctgttgctg gcggggtcga acgaggccac
     3241 catttcctgc acccattcca cgccctcggg ctggaattcg gcgttgcggt tcttcacctt
     3301 ttcgatcggc cagatgccgg tcgccaccag ggtgtagccg ggctggtagt tgtgctcctg
     3361 cttggcgtcg acgatggtga tcttggcgcc cggcaccagc tgacgcaggc ggctggccac
     3421 ggcaatgccg cccaggccgc tgccggcgat gacgatgcgc gcctggctgg aagaggtctg
     3481 cgcccgcgcg gtcggtgccg cctgcagcgc gctggccgcc agtggcgccg cgcccagcgt
     3541 gcgcagcaag tcgcgccgcg aagggcgctg atcggatcgg tgggtcatgg cttgcctcct
     3601 gtcttggggt tgaactcagg cgcctgcctt gcgcagcaga cggacatgca tggggcgttg
     3661 gaagtgggcg ccctcgggac ccacgtgggc cgcaaagcgg gcccgcacct gttcgcgcaa
     3721 gtcgtcgtcg aggtgacgct cggcataggt cacgtcgagc atgcgacgct cgaaatcggc
     3781 aaagtcggcg taccgcacgg gcaccgcaaa atgccgctcg gccgccgcct cccactggcc
     3841 cgaggccagc gcacggtcca gggccgcctg cgccgcggcg cgcacggcgc cttcgtcgtt
     3901 gtacaggcgg gtgatctggt tgagcgcgcc ggcatacacc ggctccgaca cgtacagatg
     3961 gccgccggtc cgcagcacgc ggtgcacctc acgcagcgcc tggtccatga gcgccagcgg
     4021 cacgtggtgc agcgacttga gcatcagcgc cagatcgaac tgcgcctcgg gcagcggaat
     4081 cgcctcggcg ccggcggcga tgaagctcag gcgttcggcc ggggcggcca ggtttctggc
     4141 gtgctgaatg gcgtccacct ccaggccggt cacacgcgcg tcggggtggc gggccagcag
     4201 atcgcgcgcc agccgtgcgc tgccacagcc cagctcgatg acctgcagcc cggccagcgg
     4261 caccagctcg gccagcaggt tcagttcgtc gcgttcgatg cgcgcttggg accagtccac
     4321 ggtgcttcct ttcatcgata gcgcgccagg tagcgccgct cgaagctgcg cttggcccag
     4381 tgcatcagcc gggtcggtgg cagcacgatc tggcggcgct cggtgcgcag caccagggtg
     4441 ccttcttcca gggcgtcgat gatgcagagc agctccacct tgaagccctg tgtgcccggc
     4501 tgccccgcca gttcggacag caggttgcgc gccgccgcct cggcctgcag gtctgccatg
     4561 tgggcctgct tgggcatcca gtcgggcccc ggaaagctgc cgctgtcgcc gaccacgtac
     4621 acccgctcca gctgcggcac ccggcaaccc gcatcggcgg cgatcaggcc gcccggcgag
     4681 cgtggcaggc tggattgctc aagccaggcc gggccggtca ggccgggcat gaacagaatc
     4741 agatcggccg caaactcgcc gccttcggtg accacgcggt cggcctcgaa gcgcaccatc
     4801 ttgtggccca gatgggtctg gatgccgcgc tgcgccatca tggccagcag gcgcgccacc
     4861 gtcctgctgc ccagccgggc gccaggctgc tgcgacgggt tgaagaacac cagctggaag
     4921 cgctcacgcc gcccttcgcg ccgcaactgc cgatcgatgc cgaacaggaa ctcgaacatc
     4981 ggcccgccgc gcaccgcgct gggctcgttc gggttggcgc caaagccgac ggcgatggtg
     5041 ccgccgctca tctggcgcag ccggtcacgg atgcgctcgg cggcgtcgat gccttcgcag
     5101 ggagtgatgg cgtgctcgat gcccggcagc ttgcggatga agcgcgcacc gctggcgatc
     5161 accagtgcct cgttggccag ctcgccggcc gaggtgtgca ccacccgccc gtcgtccgac
     5221 aggccggtga cgctggccag atggtggcgc acgttcatgc gggcaaagaa gttgtccagc
     5281 ggcaccacca gatcggcgcg cgtgcgcagg ccactgggta tccagatgat gccgggcagg
     5341 tagtgcagct cggcgcgcgg cgccaccagc gtgatgggcg ccgtacagcc ctgctggcgc
     5401 aggctgcgca cggtggacag ggcgccgaag ccggcgccca gcaccacgat ctgcggcgcg
     5461 gccacgccgt tcatcggggc acctccggcg cgagcgtggc gtcgacctgc gtcagcagcg
     5521 cttcgtccag ccgatcggcc agggcggcca gccgcaggcg cgtcagcacc tggtcgctgc
     5581 gcgcctgggc cagcgccagt tcggcgtcgg cctgggcgtt ttcggcgttc agcacgtcga
     5641 gcgtggtgcg gtcgcccacc tcgcgtccca ggcgggtggc gtccaggcgg gcgcggctgg
     5701 ccaccaggcc ctcctgcagc gccgccaggc gcgcggcacc ggtctgcagg ccctgccagg
     5761 tggcgcgcac ctgctgggcc acgtcctcgc gcgcgagttc gagttcggcc atggcctgat
     5821 tcaggcgttc ggcggcttcg cgctcctggg cctcacgcat gccgccggtg tagagcggga
     5881 tgttgagctg cacgcccacc atgccattca ggctgcggtt gcgtgccgaa ccgaagtcgc
     5941 caccgccggc gatgcgttcg tggcctgcct gcgccaccag gtccagcgtg gcgcggcccg
     6001 ccacgctgtg ctggcgcagc ttctgttcgg ccagcgccac cgcctgcgcg agcaggcgca
     6061 ggcgcgggtt gtcggactcg gcggccgcca gccagaacgg cagcgcttcc accggcgcca
     6121 gcggcgccag cggcagccgg gcgcggggtt gcgccaggcc ggtgctgccg gccagccggt
     6181 cgcgctgcac gctgagttcc agctcggcgg ccgactgctg cgcccgcaca ccggccaggg
     6241 cggcgtcggc ttcatgggta tcggtgatcg gcgaggcgcc cagctggtag cggtcgtgcg
     6301 cctcggcgcg cgagcgcgac acggcctgcc actggcgctc gaccacgcgc acgcgctcct
     6361 gcgccacggc cagggccagg tagcgctcgg tggtgcgcag catcagatcg gcctgcgcgc
     6421 cgcgccaggc gatgtcgccc atgtcggcgc ccagccgcag ctgagcctgc tgcgcctgcc
     6481 gcgtggcatt gacgagcggc tgctgcgcct gcaccgccac gcgcgtggcc aggccgccgg
     6541 tgaccgaggt ggcaaagcgc gcctcgtcga cctggcccat gccgggcgcc gaaaaacgcg
     6601 cgccccgcat ggcggtgtcc tgcacgccca ggcccaggcc gaggttcagg cccacctggg
     6661 ggcgccacag cgcgtcggcc tgtgcgcgca gcgtctggct ggcaccgtgt tcggcacgcg
     6721 ccacggccag gcgctgatcg tgcccggccg cagcgcgcca cacggtgagc aggtcctgcg
     6781 cccacgcgcc gaccggcacg ccgagcgcgg cacccaccag gaccagacgc gtcagacgct
     6841 tcatgccgac gccttttctc cgccgaattc ctcgtaggcg gccagcgccg aggcagcgta
     6901 catcaggccc gggccaccgc ccatatagac gcacatgccc agcatttctt cgacctcggc
     6961 gcgcgtggcg cccagcttga ccagcgcctt ggcgtggtag ccgacgcagg ggtcgcagcg
     7021 cgcgcccacc gacagcgcca tggcgatcag ctccttggtc ttggcgtcca gcgcaccggc
     7081 cttgctggcc gcctgcgcca tggcgttgaa gccctgcatg acctcgggga tgtccttgcg
     7141 cagcttggcc gaattggccg acacggcggc gatcaggttg gtgtagttct cgaccatgga
     7201 atgctccttt cagatgcctg gatgaggttc agctgcagct ctcggccggc acgccgagct
     7261 tgagcaggat gttgttgagc aggcaccatt tggtgaaacc gctttgcagc aggttggcgc
     7321 cgacgaaggc ggtgaaccag agccagttgc tgctgtggaa gatcgggctg ccctgcacgc
     7381 ccagggccag cgacagcagg atgaaggtgc ccgcgaagat acggacgata cgccaggaag
     7441 tcatgtcaat tgctcctatt taagtagctg cttgcgcagg ctgggtgggc gccagagccg
     7501 aattcttttc atgagtccgg cggtaggcgg cgaaatacag gatcgggatc accaccagcg
     7561 tcagcacggt ggacaccagg atgccgaaga tcagcgatat ggccagcccg ttgaagatcg
     7621 ggtcgtccag gatgaacagc gcgccgatca tggcggccag cgccgtcagg ccgatcggct
     7681 gcgcccggat gatggcgctc tgcacgatgg cgcgcgccag cggcatgccc tcgctcacct
     7741 gcagcttgat gaagtccacc agcaggatgg agttgcgcac gatgatgccg gccagcgcga
     7801 tcatgccgat catgctggtg gcggtgaact gcgcgcccag cagcgcgtgg cccggcatca
     7861 cgccgatgat ggtcagcggg atcggcgcca tgatgatcag cggcgtcagg taggagccga
     7921 agtgcgccac caccagcagg tagatcaaca ccagcccgac cgcgtaggcc gcacccatgt
     7981 cgcggaaggt ctcgtaggtg atctgccact cgccgtccca cttcagggcg tagccgcggt
     8041 acgggtcctg tggctggcgt atgaagtact cgtccagccg cccgccatcg ggcatcttca
     8101 gctcggccag ggcgccgcgc atctggaaca tgccgtacag cgggctgtcc agctcgccgg
     8161 cccgctggcc cgccatgtca cccatcacga agctgaccgg cagcaggtcc ttgtggtaga
     8221 tcggctgctc gcgcagcgta ttgctgggcg tgaccagctc gcggatcggc accagagcgc
     8281 cgctggccga gcgcacgccc agttgcagca gcgcgtccag gctgccgtgc agctcggccg
     8341 gcagctgaat gaaggccggc acggcaaagc ggctggcgtc gtgcaggtag gtggcggcgt
     8401 cgcccgtcag ccccatgcgc agcgtgctgg cgatggcctg ctgcgacacg cccaactgcg
     8461 ccgccttgcg acgatccacc accagcacgg tgcgcggcgc atcgacgatg gtggtgtcgt
     8521 ccacgtcgac cacgccgggc gtggcctcga acacggcgcg cacctgctgc gccacggcgc
     8581 gtcggccgtc ggcgtcgggg ccgtagatct cggccacgat tggggccagc accggcgggc
     8641 cgggcggcac ctcgaccacc ttgacgttgg cgccatgacg ctgaccgatg gcgcgcaggg
     8701 cagcgcgctc gcgcacggcg atggcgtggc tctggtcctt gcgctgcgcc ttgtcgacca
     8761 ggttgacctg caggtcgccc acttcgccgc cggcgcgcag gtagtactgg cgcaccaggc
     8821 cgttgaagtt gatcggcgcc gcggtgccgg cgtaggcctg gtagtgggtg acctcgggca
     8881 cgctggccag gtgggcgccc agctcgcgca gcaccgccgc cgtcttctcg accggcgtgc
     8941 cggccggcat gtcgaccacc acctggaact cggacttgtt gtcgaacggc agcatcttca
     9001 gcaccaccag acccagcacc ggcagcgcca ccgacagccc gatcaggccg gccaccgcca
     9061 ggcccatcag gcggcgatgg cgcgcgcccc ggcgctcgtc cagcagcggc cggaagatgc
     9121 gctcgaacag cggcgtcagc cgggccgcca ggccgtggcc ggccgcctgg ccgtggtcgg
     9181 cctggtgcgt ggccttcatc cacagccgcg ccagccaggg cgtgacgctg aaggcgatcg
     9241 ccagcgacag cagcatgccc atgctggcat tgatggggat ggggctcatg tacggcccca
     9301 tcaagccgct cacgaaggcc atcggcaaca gcgccgcgat gaccgtgaag gtggccagga
     9361 tggtcggccc gccgacttcg tcgaccgcgc cggggatgat ttgtgccagc gtgcgctccg
     9421 ggtacaggct ctggtggcgg tggatgttct cgaccaccac gatagcgtcg tcgaccaaga
     9481 tgccgatgga aaagatcagc gcgaacagcg acacgcgatt gagcgtgaag ccccaggccc
     9541 aggaggcaaa cagcgtcacc gtcagcgtca ggatcaccgc caccccgacg atggccgcct
     9601 cgcgccggcc caaggcaaag aacaccagcg ccaccaccga cagggtggca aacagcagct
     9661 tcttgatcag cgtggtggcc ttgtcgttgg cggtggcgcc gtagttgcgc gtctcggtca
     9721 cctgcacgtc ggccggcagc accgtgttgc gcaactgatc gacgcgccgc agcacggcat
     9781 cggcgacgtc gatggcgttc tcgcccggct tcttggtgat ggccagggtg accgccggct
     9841 gttccagcgc cgtggccggc gcgccgccgt caccgcgccg cacctcgcca tgccacacat
     9901 agcgcgtggg cggcggcgcg ccatcgcgca cctgagccac ttcacgcaga tacaccggct
     9961 tgccgccgcg caccgccagc accagctcgg ccacatcctg tgcgctgtgc aggtaggcgc
    10021 cggtctcgac cgccagcgcg cggttgccgc ccagcagctc gcccaccggc agaccgaggt
    10081 tggccgactg cagggcgccg tgcaactcgt gcaggctgac cccggcggcg gccaggcgct
    10141 gcgggtcggc ctcgaccatc acggcgcggc ccgggccgcc cagggtgtag acctcgcgcg
    10201 tgccgggcac gcgcttgagc tcgctctcca ggctgt
//
LOCUS       DAOOOG010000091         9996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_7744_length_9996_cov_29.281561, whole genome shotgun sequence.
ACCESSION   DAOOOG010000091 DAOOOG010000000
VERSION     DAOOOG010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7744_length_9996_cov_29.281561"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PK242_08020"
     CDS             <1..266
                     /locus_tag="PK242_08020"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOZ94002.1"
                     /translation="ALARAVAALPRLRLRGLMTIPEPAPDFEAARAVHASARALFDQM
                     NAAGLGLDTLSMGMSDDLEAAIAAGSTLVRVGSAIFGARPPKT"
     gene            complement(326..1174)
                     /gene="purU"
                     /locus_tag="PK242_08025"
     CDS             complement(326..1174)
                     /gene="purU"
                     /locus_tag="PK242_08025"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOZ94003.1"
                     /translation="MQDTYILTLSCPDQRGIVHAVSGFLMERGGNIEEAAQFNDHGTG
                     LFFMRVQFACTELDGAALRAQLPTLAERFAMRWQLHEAAQPMRTVIFVSREGHCLNDL
                     LFRWKSGLLPLDIRAIVSNHRDFYQLAASYNVPFHHIPVTREHKAQAEARQLEIIESE
                     DAELVVLARYMQILSDGMCQRLSGRAINIHHSFLPSFKGARPYWQAHERGVKLIGATA
                     HYVTADLDEGPIIEQDVARVDHSATVEDLTAVGRDTESQVLARAVKWHGEHRVLLNGQ
                     RTVVFR"
     gene            1287..2894
                     /locus_tag="PK242_08030"
     CDS             1287..2894
                     /locus_tag="PK242_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ94004.1"
                     /translation="MTAPEQPLHEHLRRHARQRPDAAAVIWYGRAISWRELDDWSDAL
                     GAHLQSLGVAPGQPVALFMNNCPQYLLAHYAIQKIGAIVCPCGPLNKAHELQYQLEDL
                     QARVILAADNLLPIVDQVRAQTALAHVLAVRYGDLLPAAPPLAVPTELQAAPAPLPAA
                     VGDLLQIAQQGAAQGQRPRPLAIGLGDVALMTYTSGTTGLPKGAMLSYGNALFKTQVT
                     AQSAGIGAGDVALSVAPLYHIAGMLMGVNVPVLAGATQVLLYRFDPLAVLQAIDRYRV
                     THWYSIAPMNVACMQVPDAGRFDLGSLRRNPVTSFGITFTQALAEQWQAFTRGPCLSY
                     EAAYGLSETHTMDTGMPPEAIRWGTHGQPMPGVSIRIVDPDSGVEKPVGETGEITVRS
                     PGNFKGYWNKPEASARTLRDGWVWTGDMGRMDAEGYLTFIGRTKEMIKVSGYSVFPEE
                     VESLLIRHPAVAQAAVLGEPDPDKGEVVKAFIVLKPGASLTADALIAWSRDNMAPYKA
                     PRHVRFIDALPATGAGKVLRRLLTELD"
     gene            3027..6317
                     /locus_tag="PK242_08035"
     CDS             3027..6317
                     /locus_tag="PK242_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOZ94005.1"
                     /translation="MFHKVLIANRGEIALRIVRALQDLGLPSLAVYADDDAQAPHVRS
                     ADQAVALGASGPAAYLDGARLLAIARAHGADALHPGYGFLAERADFARDCAAAGVRFI
                     GPTPEQLALCGDKAQARALAERCGVPLLPGTQQAVTLAQAQAFFEQHAQAGIVIKAVA
                     GGGGRGMRAIEHAAELPAAYARCRSEALAAFGVADVYVERLMRRARHIEVQVLGDGHD
                     VMALGERECTLQRRFQKLVEIAPSPSLPAPLRQRLVDSALRMARELRYQGLGTFEFLV
                     DAASDTLPYVFIEANARLQVEHTITEAVTGVDLVQTQLRLAAGQRLADAGLDVAAPPP
                     PRGHAIQWRINAETLAADGSATPGSGRLTRFELPAGPGVRVDTHGVAGATPSPHYDSL
                     LAKLVVHAPGSDFAEVLRRSHRALADTRIDGVATNLALLRALCRRPEMASQQVHTRWL
                     EAALPELLNTAKTIAAAADQESAAGQNDAQAPQAVEAAEAPQGALLAPMPARLVQLSV
                     AVGDAVAAGAELAVLEAMKMEHVLTAPHAGVLRQCFVAPGAYVAQGDVLLVLEPADVS
                     DAAQQAAAAAHDPDHIRADLQRVLERHAFTLDAARPEAVARRHARGGRTVRENIADLC
                     DEGSFIEYGALAVAAQTRRRSMADLIANTPADGMVTGIGGVNGLIFGAEAARTAVMGY
                     DATVLAGTQGMRNHAKTDRLLGIALDQQLPVVLLAEGGGGRPADVDVPVVAGLHVGTF
                     AAYAALSGRVPVVGVVHGYCFAGNAALLGCSDVIIATRASHIGMGGPAMIEGGGLGVF
                     APEQIGPSGVQHGNGVIDLLVDDEAAAVAAARHYLSYFQGRVRDWVTPDQRLLRAVVP
                     ENRLRVYDSRDAMAGLVDQGSLLALRTGFGAGVHTALARIEGRPVGLIANNPLHLGGA
                     IDAAAADKAARFMQLCNAHGLPIVSLIDTPGFMVGPEVEQTAQVRHVSRLFVTAASLR
                     VPFFSVVLRKGYGLGAMAMAAGGFHAPVFTLSWPTGEFGGMGLEGYVRLGFRKELEAA
                     AEGAERDALFARLLAERYEHGAALNMATTLEIDAVIDPAETRAWLARGLASARLRPAT
                     GSRFVDTW"
     gene            complement(6391..7206)
                     /locus_tag="PK242_08040"
     CDS             complement(6391..7206)
                     /locus_tag="PK242_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOZ94006.1"
                     /translation="MRRLILLATLVLAACALPPLDAGQQARLATLLPTDALLLGEQHD
                     APEHQHLQRQVVQWLGDRGQLAAVVLEMAEGGRSTAGLPRDARAHQVRAALGWDDARW
                     PWQVYGPVIMRAVAAGAPVQGANLSPAQMREAMRQSALDARLPPALLAVQRQRIRASH
                     CGLLPERQIDPMTRVQIARDIAMADAVAGAARQAAPGRVVLLITGNGHVHRALGVPRF
                     LPPSLKSKVLSAQAEQAQAAPENEALAGTALQPGDLLWPTPALPPRDPCTTLR"
     gene            7238..8320
                     /locus_tag="PK242_08045"
     CDS             7238..8320
                     /locus_tag="PK242_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HOZ94007.1"
                     /translation="MPSSAVPPFVRAALTLLLAWGAARLGVWLSLPVPWMLGPLVATA
                     LASMAGLPTHSVRALRNAGQWVIGVSLGLYFTPAMAALMLGHWWALLLGVLWALALGL
                     GFAAWLRRATRASLGELSPAERAATLHFASTIGGASEMTLLAERAGARTDLVAAAHSV
                     RMAVVVLSLPFAMQWARQHWQLPALDVLPAAERVVDGPGLLWLALATGAGAALLRRTG
                     GANPWFIGPLAVAMALTMLDRHLSAVPPALGQAAQLVIGVSLGVRFSRRFLHAAPRWL
                     AAVALGTLGMIALCAAMAWVLAWATGLHPVTLMLAASPGGLAEMAITAQVLQLGAPVV
                     TAFQVCRLVAVLLLAEPVFRWGSARR"
     gene            complement(8332..8613)
                     /locus_tag="PK242_08050"
     CDS             complement(8332..8613)
                     /locus_tag="PK242_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HOZ94008.1"
                     /translation="MNTLYDSDSFAVVHLLPDVLADTPAQAGQPVLARHGFEIVDKRS
                     GKEVYLDGLWAELFQQRIAAWQQNTPTQEEVEETLEGYAELAQNPVVMH"
     gene            complement(8746..9783)
                     /locus_tag="PK242_08055"
     CDS             complement(8746..9783)
                     /locus_tag="PK242_08055"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOZ94009.1"
                     /translation="MSRQPPPHRARPPLALVGAVLLAHVLLLRPAPPRATPAPAPRAA
                     PLWLRAIPAPAATPAVTPVASAGATPPAPGRATSPAGTARTPAVTAPSPRRLTPLARA
                     SAAAPPPRTETDGRQTPAVPAAPAVAVAAPVRLADTVTLHYRVSGLARGLPLQADAQL
                     RWQREGSHYEAEWTLQLPPIGTRRQHSTGRVTPAGLVPERYAERQRGERAAHFDAEGG
                     RIRFSANTPDATLQAGAQDRLSTLLQLGGLIAADPPRYPPGSQIVLQTAGAREAADWR
                     WEVLDDEVLQLDGQAVPCVRLQRAPRHEYDTRIELWLARALHYLPARLRVTQASGDVA
                     DQQLSSLPTPR"
     gene            9900..>9996
                     /locus_tag="PK242_08060"
     CDS             9900..>9996
                     /locus_tag="PK242_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94010.1"
                     /translation="MKLATYKDGSRDGQLVVVSRDLTTAHYATGIA"
BASE COUNT         1288 a         3553 c         3722 g         1433 t
ORIGIN      
        1 tggcgctggc gcgcgccgtc gccgcgctgc cgcgactgcg cctgcgcggg ctcatgacga
       61 tccccgaacc tgctcctgat tttgaagctg ctcgcgcagt ccacgcaagc gccagagccc
      121 tgtttgatca gatgaatgcg gccggcctgg ggctggacac gctgtcgatg ggcatgagcg
      181 acgatctgga agcggcgatc gccgccggct cgaccctggt gcgggtgggc agcgcgatct
      241 tcggcgcccg cccgcccaag acctgagagg gggtgaacca accccctcag gcccggcttc
      301 ggcggcatcg gcccgctggc cggcctcagc ggaacaccac cgtgcgctgc ccgttgagca
      361 gcacccggtg ctcgccgtgc catttgacgg cgcgcgccag cacctggctt tcggtgtcgc
      421 ggccgacggc ggtcaggtct tccaccgtgg cgctgtggtc cacgcgcgcc acgtcctgct
      481 cgatgatcgg accttcgtcc aggtcggccg tcacgtagtg ggcggtggcg ccgatcagct
      541 tgacgccgcg ctcgtgcgcc tgccagtatg gccgcgcgcc cttgaagctg ggcaggaagc
      601 tgtggtggat gttgatggcg cgcccggaaa gccgctggca catgccgtcc gacaggatct
      661 gcatgtagcg cgccagcacc accagttcgg cgtcttcgga ctcgatgatc tccagttgcc
      721 gcgcctcggc ctgcgccttg tgctcgcgcg tgacggggat gtggtgaaac ggcacgttgt
      781 agctggccgc cagctggtag aagtcgcggt ggttgctgac gatggcgcgg atgtccagcg
      841 gcagcaggcc gctcttccag cggaacagca ggtcgttcag gcaatggccc tcgcggctga
      901 cgaagatcac ggtgcgcatg ggctgcgccg cctcgtgcag ctgccagcgc atggcaaagc
      961 gctcggccag cgtcggcagc tgggcgcgca gcgcggcgcc gtccagctcg gtgcaggcga
     1021 actgcacccg catgaagaac aggccggtgc cgtggtcgtt gaactgcgcg gcctcctcga
     1081 tgttgccgcc gcgctccatc agaaagcccg agacggcatg cacgatgccg cgctggtccg
     1141 ggcaggacag ggtcaggatg taggtgtctt gcatcgggcg cgattgtcgc aggcgacgcg
     1201 ctgctgtcac ctgcgcgatc gttcacgggc ggccaggtgc ccccggctcg ctatattggt
     1261 tttttgcctc attcatggag acctgcatga ccgcccccga gcagcccctg cacgagcacc
     1321 tgcgccgcca cgcgcggcag cggcccgacg ccgccgccgt gatctggtat ggccgcgcca
     1381 tcagctggcg cgagctggac gactggagcg acgcgctggg cgcccacctg cagtcgctgg
     1441 gcgtggcgcc gggccagccg gtggcgctgt tcatgaacaa ctgcccgcag tacctgctgg
     1501 cgcactacgc gatccagaag atcggcgcca tcgtctgccc ctgcgggcca ctgaacaagg
     1561 cgcacgagct gcaataccag ctggaggatc tgcaggcacg cgtcatcctg gccgccgaca
     1621 acctgctgcc catcgtcgat caggtgcgcg cgcagaccgc actggcgcat gtgctggcgg
     1681 tgcgctatgg tgatctgctg ccggccgcgc cccccctggc cgtgccgacc gagctgcagg
     1741 ccgcgccggc cccgctgccg gccgcggtgg gcgatctgct gcagattgcg cagcagggcg
     1801 cggcgcaggg ccagcgcccg cggccgctgg ccatcgggct gggcgacgtg gccctgatga
     1861 cctacaccag cggcaccacc ggcctgccca agggcgccat gctgagctac ggcaacgcgc
     1921 tgttcaagac ccaggtcacc gcccagtcgg ccggcatcgg cgccggcgat gtggcgctgt
     1981 cggtggcgcc gctctaccac atcgccggca tgctgatggg cgtcaatgtg ccggtgctgg
     2041 ccggcgccac ccaggtgctg ctgtaccgct tcgacccgct ggcggtgctg caggcgatcg
     2101 accgctatcg ggtcacgcac tggtattcga tcgcgcccat gaacgtggcc tgcatgcagg
     2161 tgcccgatgc gggccgcttc gacctgggca gcctgcggcg caacccggtc accagtttcg
     2221 gcatcacctt cacgcaggcg ctggccgagc agtggcaggc cttcacccgg ggcccgtgcc
     2281 tgagctacga agccgcctac gggctgtcgg aaacccacac catggacacc ggcatgccgc
     2341 ccgaggcgat ccgctggggc acccacggcc agcccatgcc gggcgtcagc atccgcatcg
     2401 tcgaccccga cagcggcgtt gaaaagccgg tcggcgaaac cggcgaaatc acggtgcgct
     2461 cgccaggcaa cttcaagggc tactggaaca agcccgaagc cagcgccagg acgctgcgcg
     2521 acggctgggt ctggaccggc gacatgggcc gcatggacgc cgagggctac ctgaccttca
     2581 tcggccgcac caaggagatg atcaaggtgt cgggctacag cgtgttcccg gaagaggtgg
     2641 aatccctgct gatccgccac cctgcggtgg cgcaggcggc ggtgctgggc gagcccgacc
     2701 ccgacaaggg cgaggtggtc aaggccttca tcgtgctcaa gcccggcgcc agcctgacgg
     2761 ccgacgcgct gatcgcctgg agccgcgaca acatggcccc ctacaaggcg ccgcgccatg
     2821 tgcgcttcat cgacgcgctg ccagccaccg gcgcgggcaa ggtgctgcgg cggctgctga
     2881 cagagctgga ctgaagacag ccctccgtac gcagagggcg cagaggttgc gcagaggacg
     2941 caaaaccatt cctcgaagtt ggttctgcgc cttctgcatg atctttgcgt cctctgcgta
     3001 tggatgttca tgcatccccc cacaccatgt tccacaaagt tctgatcgcc aaccgcggcg
     3061 aaattgccct gcgcatcgtg cgcgccctgc aggatctggg cctgcccagc ctggccgtgt
     3121 atgccgacga cgacgcgcag gcgccgcatg tgcggtcggc cgaccaggcg gtggcgctgg
     3181 gcgccagcgg gccggcggcc tatctggacg gcgcgcgcct gctggccatc gcgcgcgcgc
     3241 acggcgccga cgccctgcac cccggctacg gctttctggc cgagcgcgcc gacttcgcgc
     3301 gcgactgcgc ggcggccggc gtgcgcttca tcggccccac gcccgagcag ctggcgctgt
     3361 gcggcgacaa ggcgcaggcg cgcgccctgg ccgagcgctg cggcgtgccc ctgctgcccg
     3421 gcacgcagca ggcggtgacg ctggcgcagg cgcaggcgtt ctttgagcag cacgcgcagg
     3481 ccggcatcgt catcaaggcg gtggcgggcg gtggcgggcg cggcatgcgc gccatcgaac
     3541 acgccgccga gctgcccgcc gcctacgcgc gctgccgcag cgaggcgctg gcggccttcg
     3601 gtgtcgccga cgtgtatgtg gagcgcctga tgcgccgcgc gcgccacatc gaggtgcagg
     3661 tgctgggcga cggccacgac gtgatggcgc tgggcgagcg cgaatgcacg ctgcagcggc
     3721 gcttccagaa gctggtcgag atcgccccca gccccagcct gccggcgccg ctgcgccagc
     3781 gcctcgtcga cagcgccctg cgcatggcgc gcgagctgcg ctaccagggc ctgggcacgt
     3841 tcgaattcct ggtcgatgcg gcatctgaca cgctgcccta tgtgttcatc gaggccaacg
     3901 cgcgcctgca ggtcgagcac accatcaccg aggcggtgac cggggtcgac ctggtgcaga
     3961 cccagctgcg gctggccgcc ggccagcgcc tggccgacgc cgggctggac gtcgccgcgc
     4021 cgccgccgcc gcgtggccac gccatccagt ggcgcatcaa cgccgagacc ctggccgccg
     4081 acggcagcgc caccccgggc agcggccggc tgacgcgctt cgagctgccg gccggcccgg
     4141 gcgtgcgggt ggacacgcac ggcgtggccg gtgccacgcc ctcgccgcac tacgacagcc
     4201 tgctggccaa gctggtcgtg cacgcgcccg gcagcgactt tgccgaggtg ctgcgccgct
     4261 cgcaccgcgc gctggccgac acccgcatcg acggcgtggc caccaacctg gcgctgctgc
     4321 gcgcgctttg ccgcaggccc gaaatggcca gccagcaagt gcacacgcgc tggctcgaag
     4381 cggccctgcc ggaactcctg aatactgcaa aaacaatagc tgctgccgca gaccaggaaa
     4441 gcgctgcagg ccagaatgat gcccaagccc cgcaggcggt cgaggccgcc gaggcaccgc
     4501 aaggcgcgct gctggcgccg atgccggcgc gcctggtgca gctgtcggtg gcggtcggcg
     4561 atgcggtggc ggccggcgcc gaactggcgg tgctggaagc catgaagatg gagcatgtgc
     4621 tcaccgcgcc ccacgccggc gtgctgcgcc agtgcttcgt cgcgcccggc gcctacgtgg
     4681 cgcagggcga cgtgctgctg gtgctggaac cggccgacgt gagcgacgcc gcgcagcagg
     4741 ccgcggccgc cgcccacgac cccgaccaca tccgcgccga cctgcagcgc gtgctggagc
     4801 gccacgcctt cacgctggac gccgcgcgcc ccgaggcggt ggcgcggcgc cacgcgcgcg
     4861 gcgggcgcac cgtgcgcgag aacatcgccg acctgtgcga cgaaggcagc ttcatcgaat
     4921 acggcgcgct ggcggtggcg gcgcagacgc gccggcgcag catggccgac ctgattgcca
     4981 acaccccggc cgacggcatg gtgaccggca tcggcggcgt caacggcctg atcttcggcg
     5041 ccgaggccgc gcgcaccgcg gtgatgggct acgacgccac cgtgctggcc ggcacgcagg
     5101 gcatgcgcaa ccatgccaag accgaccgcc tgctgggcat cgcgctcgat cagcaactgc
     5161 cggtggtgct gctggccgaa ggcggcggcg gccggccggc cgacgtcgac gtgccggtgg
     5221 tggccggcct gcacgtgggc accttcgccg cctacgccgc gctgtcgggc cgggtgccgg
     5281 tggtcggggt ggtgcacggc tactgctttg ccggcaacgc cgccctgctg ggctgcagcg
     5341 acgtcatcat cgccacccgc gccagccaca tcggcatggg tgggccggcc atgatcgaag
     5401 gcggcggcct gggcgtgttc gcgcccgagc agatcggccc cagcggcgtg cagcacggca
     5461 acggcgtgat cgatctgctg gtcgatgacg aagcggccgc ggtggcggcg gcgcggcact
     5521 acctgtcgta ctttcagggc cgggtcaggg actgggtcac gcccgatcag cggctgctgc
     5581 gcgcggtggt gccggaaaac cggctgcgcg tgtacgacag ccgcgacgcc atggccggcc
     5641 tggtcgatca gggcagcctg ctggcgctgc gcaccggctt cggcgccggc gtgcacaccg
     5701 cgctggcgcg catcgaaggg cggccggtgg gcctgatcgc caacaacccg ctgcacctgg
     5761 gtggcgccat cgacgccgcc gcggccgaca aggcggcgcg cttcatgcag ctgtgcaacg
     5821 cccacggcct gccgatcgtc agcctgatcg acacgcccgg cttcatggtc ggcccggagg
     5881 tggaacagac ggcccaggtg cgtcacgtca gccgcctgtt cgtcaccgcc gccagcctgc
     5941 gcgtgccgtt cttcagcgtg gtgctgcgca agggctacgg cctgggcgcc atggcgatgg
     6001 cggcgggcgg cttccatgcg ccggtgttca cgctgtcctg gcccaccggc gagttcggcg
     6061 gcatggggct ggagggctat gtgcggctgg gttttcgcaa ggagctcgaa gccgcggccg
     6121 agggcgccga gcgcgacgcc ctgttcgccc gcctgctggc cgaacgctac gagcacggcg
     6181 ccgcgctcaa catggccacc acgctggaga tcgatgccgt catcgacccg gccgagacgc
     6241 gcgcctggct ggcgcgcggg ctggcgtcgg cgcgcctgcg cccggcgacg gggtcgcgct
     6301 tcgtcgatac ctggtagcgc agcccgcgcc cgtggcgccc ggctggcgcc gaacgcgcca
     6361 cggcggggcg tgccgccgtc gccgtggcgc tcagcgcagc gtggtgcagg ggtcgcgcgg
     6421 cggcagcgcc ggggtcggcc acagcaggtc gccgggctgc agggcggtgc ccgccaatgc
     6481 ttcgttttca ggagctgcct gcgcttgctc cgcctgcgct gaaagcactt ttgacttcaa
     6541 gctcgggggc agaaagcgcg gcacgcccag cgcgcggtgc acatggccgt tgccggtgat
     6601 cagcagcacc acgcggccgg gcgcggcctg gcgtgcggcg ccggccacgg catcggccat
     6661 ggcgatgtcg cgcgcgatct gcacccgcgt catggggtcg atctggcgct ccggcagcag
     6721 gccgcagtgg ctggcgcgga tgcgctggcg ctgcaccgcc agcagcgccg gcggcaggcg
     6781 cgcgtccagc gcgctctggc gcatggcctc gcgcatctgc gctggcgaca ggttggcccc
     6841 ctgcaccggc gcgccggccg ccaccgcgcg catgatcacc gggccgtaca cctgccacgg
     6901 ccagcgcgcg tcgtcccagc ccagcgcggc gcgcacctgg tgcgcacggg cgtcgcgcgg
     6961 caggccggcg gtgctgcgtc cgccttcggc catttccagc accactgcgg ccaattggcc
     7021 gcgatcgccc agccactgca ccacctggcg ctgcaggtgc tggtgttcgg gcgcgtcgtg
     7081 ctgttcgccc agcagcaggg cgtcggtcgg cagcagggtg gccagccttg cctgctggcc
     7141 ggcatccagc gggggcaggg cgcaggccgc cagcaccagg gtggcaagca ggatcaggcg
     7201 tcgcatgggg gcgatactaa gcggctggcc ggtcgtcatg ccttcgtctg ccgttccccc
     7261 cttcgtgcgc gctgcgctga ccctgctgct ggcctggggc gcggcgcggc tgggcgtgtg
     7321 gctgtcgctg ccggtgccct ggatgctggg ccccttggtg gccaccgccc tggccagcat
     7381 ggccggcctg ccgacgcaca gcgtgcgcgc cttgcgcaac gccggccagt gggtgatcgg
     7441 ggtcagcctg gggctgtact tcacgccggc gatggcggcg ctgatgctgg gccattggtg
     7501 ggcgctgctg ctgggcgtgc tgtgggcact ggccctgggg ctgggctttg ccgcctggct
     7561 gcggcgggcc acgcgcgcca gtctgggcga gctgagcccg gcggagcgcg ccgccacgct
     7621 gcactttgcc agcaccatcg gcggcgcctc cgagatgacc ctgctggccg agcgcgccgg
     7681 cgcccgcacc gacctggtgg cggccgcgca cagcgtgcgc atggcggtgg tggtgctgag
     7741 cctgccgttt gccatgcagt gggcgcgcca gcactggcag ctgccggcgc tggacgtgct
     7801 gccggcggcc gagcgggtgg tcgacgggcc ggggctgctg tggctggcgc tggcgaccgg
     7861 ggccggcgcc gccctgctgc ggcgcaccgg cggcgccaac ccctggttca tcggcccgct
     7921 ggcggtggcg atggcgctga ccatgctgga ccgccacctg tcggccgtgc cgccggcgct
     7981 gggccaggct gcgcagctgg tgatcggggt cagtctgggg gtgcgctttt cgcgccgctt
     8041 cctgcacgcg gcgccgcgct ggctggcggc cgtggcgctg ggcacgctgg gcatgatcgc
     8101 gctgtgcgcg gccatggcct gggtgctggc ctgggccacc gggctgcacc cggtgacgct
     8161 gatgctggcc gcctcgcccg gcgggctggc cgaaatggcc atcaccgccc aggtgctgca
     8221 actgggcgcg ccggtggtga cggcgtttca ggtgtgccgg ctggtggcgg tgctgctgct
     8281 ggccgagccg gtgttccggt gggggtcggc gcggcgctga cgcgaacgcg ctcagtgcat
     8341 cacgaccggg ttctgcgcca gttcggcgta gccttccagg gtttcctcga cttcttcctg
     8401 ggtgggggtg ttttgctgcc aggcggcgat gcgctgctgg aacagctcgg cccacaggcc
     8461 gtcgaggtag acctccttgc ccgagcgttt gtccacgatt tcgaagccgt ggcgcgccag
     8521 cacgggctgg cccgcctgcg ccggcgtatc ggccagcacg tcgggcaaca ggtgaacgac
     8581 cgcaaagctg tcggaatcgt agagcgtgtt catcggtggt caggcccctc ggatggcagt
     8641 ggttgatgcg aaagtctcac tggcctgcat atgagaacgc atcgggcgct ttcaaggtag
     8701 acgcccgcca ggcggaaaac ccaattaagt ccggtttcga aggcctcagc gcggcgtcgg
     8761 caggctggac agttgctgat cggccacatc gccgctggcc tgggtgacgc gcaggcgcgc
     8821 cggcaggtag tgcagcgcac gcgccagcca cagctcgatg cgcgtgtcgt actcgtgccg
     8881 gggcgcgcgc tgcaggcgca cgcagggcac ggcctgcccg tcgagctgca gcacttcgtc
     8941 gtccagcact tcccagcgcc agtcggcggc ctcgcgcgcg ccggcggtct gcagcacgat
     9001 ctggctgccg ggcgggtagc gtggcgggtc ggcggcgatc aggcccccca gctgcagcag
     9061 cgtgctcagg cggtcctggg cgccggcctg cagggtggcg tccggcgtgt tggcggaaaa
     9121 gcggatgcga ccgccctcgg cgtcgaaatg cgcggcgcgc tcgccccgtt ggcgctcggc
     9181 gtagcgctcg ggcaccagcc cggccggtgt gacgcggccg gtgctgtgct gccggcgcgt
     9241 gccgatgggc ggcagttgca gcgtccactc ggcctcgtag tgcgagcctt cgcgctgcca
     9301 gcgcagctgt gcgtcggcct gcagcggcag gccgcgcgcc aggccgctga cgcggtagtg
     9361 cagggtgacg gtgtcggcca gccgcaccgg cgccgccacg gcgacggccg gtgccgcggg
     9421 caccgcgggt gtctgtcgac cgtccgtctc ggtgcgcggc ggtggtgccg ctgcgctggc
     9481 gcgcgcgagc ggcgtcaggc gccgcgggct gggcgcggtc acggccggcg tgcgcgcagt
     9541 gccggccggg gacgtggcgc ggccgggggc gggtggcgtc gcgccggcgc tggcgacggg
     9601 cgtgacggcc ggcgtggcgg cgggcgccgg gatggcgcgc agccacagcg gcgcggcgcg
     9661 gggggcaggc gcgggcgtgg cccggggcgg cgcgggccgc agcagcagga catgcgccag
     9721 cagcacggcg cccaccagcg ccagcggcgg acgcgcgcga tgcggcggcg gctggcggct
     9781 catgcactca acactagaat cgccgcttgc gggcgcgcac cgatggccgc gcccgcgccc
     9841 cgcccgccgg tccgcgccgg ccgtgcgtct gtgcggcggg gcttcgctga cacgcatcca
     9901 tgaaactggc aacctacaaa gacggctcgc gcgacgggca gctggtggtc gtctcgcgcg
     9961 acctgacgac ggcccattat gcgaccggca tcgcca
//
LOCUS       DAOOOG010000092         9981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_7768_length_9981_cov_29.871146, whole genome shotgun sequence.
ACCESSION   DAOOOG010000092 DAOOOG010000000
VERSION     DAOOOG010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9981
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7768_length_9981_cov_29.871146"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1802
                     /gene="aceK"
                     /locus_tag="PK242_08065"
     CDS             <1..1802
                     /gene="aceK"
                     /locus_tag="PK242_08065"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HOZ94011.1"
                     /translation="ARAKHRFETCDWHGQQRAQRERIEFYDLRVRECQKRLEREFRAH
                     QQPMAVWHQVKLLYIGLLVTHPQPELAETFFNSVTTRILHRSYFHNDFIFVRPAISTE
                     YMETTDPKAPPTFRAFYPGDAPLAHTVRAMLESCELRCRWDDMERDAGLVARAMQREL
                     GTARPRSNFQLQVLSSLFYRNKGAYLVGKLINGFQEIPFALPILHRQPGTLHVDAALF
                     GEDDLAILFSFSRAYFMVDMEIPSAYVQFLRSLMPRKPRAELYNALGLAKQGKNLFYR
                     DLLWHLRHSSDQFRIAPGIKGMVMLVFDLPSFPYVFKLIRDRFAAPKETTREQVRAKY
                     QLVKRHDRVGRMADTMEFSLLAFPRQRFTDELVAELEQHAASLLEISDRDGDGETEVI
                     IAHAYIERRMIPLNLYLQEALDAGAGDAAASAQLEHAVIEYGNAIKDLVAANIFPGDM
                     LWKNFGVTRHGKVVFYDYDEIEYVTDCQFRRVPPPRHEEDELSGEVWYQVARNDVFPE
                     TFAPFLLGHPRVREVFLRHHADLLTPEFWQQNKERIQRGEVIDFFPYGTHRRFDCNGG
                     VAGAPELADPLAAHHETPSGPPDALAPLAPVPE"
     gene            1854..3035
                     /locus_tag="PK242_08070"
     CDS             1854..3035
                     /locus_tag="PK242_08070"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOZ94012.1"
                     /translation="MSDPIVIVSAARTPMGSFQGDFSSLAAHDLGGAAIRAAIERAGI
                     QPELVTEVLFGNCLLAGQGQAPARQAAFKGGLPKSAGAVTLSKMCGSGMRAAMFAHDM
                     LVAGSHDVLVAGGMESMTNAPYLLQKGRGGYRMGHDKVYDHMMLDGLEDAYEPGRSMG
                     TFGEDCAARYHFTREQQDAFATASVRRAQAAIESGAFQAEITPVTVKERKGERVVDTD
                     EGPGRIKLDNIPKLKPAFKKDGTVTAASSSSINDGAAAMVLMRESTARKLGCRPLARI
                     ASHATHAQEPEWFSTAPIGATEKALAKAGWQVGDVDLWEINEAFAVVPMALMHDLKVP
                     HEKVNVNGGACALGHPIGASGARIMVTLIHALKARGLKKGLATLCIGGGEATAVALEM
                     L"
     gene            3109..3747
                     /locus_tag="PK242_08075"
     CDS             3109..3747
                     /locus_tag="PK242_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOZ94013.1"
                     /translation="MIDSLDALRALYPEASARSLAKQLDRLDEAMCRFVAHAPLCVVA
                     SVGVGGALADASPRGGLPGFVRVPDARTLWIPDATGNNRLDTLENLLADPRIGLLFLV
                     PGVDEVLRVNGTARLRDEDVFTQRFAAEGVKAPRLVIEVAVREAYLHCPKALLRARLW
                     SPEVRAAPGTFPSMNAMLRAQLGSAVITETDEQARERYRAQLADEGLAHHVR"
     gene            3760..4431
                     /locus_tag="PK242_08080"
     CDS             3760..4431
                     /locus_tag="PK242_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ94014.1"
                     /translation="MPTTLILGASRGIGLELARQSLAAGERVIATARSDAGLQTLRAL
                     GAQALRLDVAEPASVSGLAWQLDGEQIDSAWYVAGVMCARADARQPPTAPAFDRVMHT
                     NVLGAMQVIPQVAPLVEAAGGRFGFISSTMGRIGDVEASNAWLYRVSKAALNMVVASA
                     RHSYPRAVLAAFHPGWVQTDMGGAQAPVTVTDSVAGLRRTLAALTLADSGAFLRYDGQ
                     RLASW"
     gene            complement(4504..5073)
                     /locus_tag="PK242_08085"
     CDS             complement(4504..5073)
                     /locus_tag="PK242_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOZ94015.1"
                     /translation="MTPAPDPVDAAFAAQPREGFLPLDQRPLAALDRPLPIGWGQTNS
                     QPSTVANMLRLLQVAPGMRVLDVGAGSGWTTALLAHLVGPQGQVHGVERVPELVRFGA
                     DNLAATGQPWARIVAAEPGQLGLPALAPFDRILVSAEADRLPQALVDQLADHGLMVIP
                     VNGSMHSVRRRGDELELGTHGEYRFVPLR"
     gene            complement(5102..5368)
                     /gene="infA"
                     /locus_tag="PK242_08090"
     CDS             complement(5102..5368)
                     /gene="infA"
                     /locus_tag="PK242_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOZ94016.1"
                     /translation="MAKEELIEMQGIVNEVLPDTRFRVTLDNGHELVAYSAGKMKKNH
                     IRILAGDRVSLELSPYDLTKGRISFRHLERRGPMPTGGGGQRRR"
     gene            5854..6195
                     /locus_tag="PK242_08095"
     CDS             5854..6195
                     /locus_tag="PK242_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94017.1"
                     /translation="MTPRRLSRGAAVVLMTTALAWNLPAAAQIAPASDGGATAVTSTP
                     EAARASVPPAAAAGQPGPGERARGGTFGGGRAASAAADPVAPAPTPPASGPATGADVP
                     PPPAIHAPGKS"
     gene            complement(6282..7007)
                     /locus_tag="PK242_08100"
     CDS             complement(6282..7007)
                     /locus_tag="PK242_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94018.1"
                     /translation="MTVPADPAEEPSLRPPEAEPQRLGSWLLAAVLVLVIVGGAWRAY
                     QWLVTDVERRRAVAEAPPPASAAPAVVAASAAPAPLGRTVTAPQAGVPPEAAAPAAVG
                     QAIHRCRIDGQLTYTNLPCAPGTEDLPAPALGADPNGVVGLAGDSVPPVQPAELSLEG
                     ASRSQRDAACAYLAAEIARLEFEFQQPLPPPVLDHLSTRLSGLRARQRAAECGAPAVP
                     AADQASATRRPASPRAADARRAP"
     gene            complement(7170..7436)
                     /gene="minE"
                     /locus_tag="PK242_08105"
     CDS             complement(7170..7436)
                     /gene="minE"
                     /locus_tag="PK242_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOZ94019.1"
                     /translation="MSFLSFFLGEKKKTASVAKERLQIILAHERAGRSAAEPDYLPDL
                     QRDLISVISKYVRIDPKDIKVHLERQDDLEVLEVKIELPEKKPD"
     gene            complement(7441..8256)
                     /gene="minD"
                     /locus_tag="PK242_08110"
     CDS             complement(7441..8256)
                     /gene="minD"
                     /locus_tag="PK242_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOZ94020.1"
                     /translation="MARIVVVTSGKGGVGKTTTSAAFASGLALKGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDFVNVIHGEANLTQALIKDKQCDNLFVLAASQTRDKDALTKDGV
                     EKVLKDLAAMDFDYIVCDSPAGIETGALLAMHFADEALIVTNPEVSSVRDSDRILGML
                     GSKTQRAIEGKDPVKEHLLITRYNPNRVADGQMLSLDDIQEILRVKLIGVIPESEAVL
                     QASNQGLPAVHLKGSDIAGAYMDVIERFLGAEVPMRYVEAEKPGFFKRLFGGR"
     gene            complement(8301..9059)
                     /gene="minC"
                     /locus_tag="PK242_08115"
     CDS             complement(8301..9059)
                     /gene="minC"
                     /locus_tag="PK242_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOZ94021.1"
                     /translation="MSVAVAGRSPTTFDIKSASLPLLTLRLKSPDLARLAEELLAQYG
                     DRPDFFEDDAVVLDLSTLPAEQAGAEIDFQQLGELLQARRLRLLAVRGGSAAQHEAAR
                     AIGLLQAPDLRVAQAAAPDAAPARRADSAAVEPPPPGALVIERPLRSGQQVYARGRDL
                     VLLAMVNAGAEVIADGHIHVYAPLRGRAIAGARGWAEARVFALEMRPDLVSIAGVYRT
                     GDEPLPPSVWGQRASVRLASGEVGDKLIFEPVAG"
     gene            9494..>9981
                     /locus_tag="PK242_08120"
     CDS             9494..>9981
                     /locus_tag="PK242_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94022.1"
                     /translation="MISIVLPFFRKLADFRRVLPLNRPYFARPWVEVVVVMDENESES
                     GVLDLIRQYPDIRWQVRVNDQPHGWQPPCKAINVGVRHASHEVVLVCSPESMFVTDVP
                     AQALGTVRAVPRGVALGRVGFGQYAQLHQLGASAAFDAVAPDARDPFKLYGSIALARQ
                     EL"
BASE COUNT         1523 a         3465 c         3462 g         1531 t
ORIGIN      
        1 cggcgcgcgc caagcaccgc ttcgagacct gcgactggca cggccagcag cgcgcccagc
       61 gcgagcgcat cgagttctat gacctgcggg tgcgcgaatg ccagaagcgg ctggagcgcg
      121 agttccgcgc ccatcagcag cccatggccg tgtggcatca ggtcaagctg ctgtacatcg
      181 gcctgctggt gacgcacccg cagcccgaac tggccgaaac cttcttcaac tcggtcacca
      241 cgcgcatcct gcaccgcagc tatttccaca acgacttcat cttcgtgcgc ccggccatca
      301 gcaccgagta catggagacc accgacccca aggcgccgcc caccttccgc gccttctacc
      361 ccggcgacgc cccgctggcg cacacggtgc gcgccatgct cgaatcctgc gagctgcgct
      421 gccgttggga cgacatggaa cgcgacgccg gcctggtggc gcgcgccatg cagcgcgagc
      481 tgggcaccgc caggccgcgc tccaacttcc agctgcaggt gctgagcagc ctgttctatc
      541 gcaacaaggg ggcctacctg gtcggcaagc tgatcaacgg ctttcaggaa atcccgttcg
      601 ccctgcccat cctgcaccgc cagcccggca cgctgcacgt cgacgccgcg ctgttcggcg
      661 aggacgacct ggccatcctg ttcagcttct cgcgcgccta cttcatggtg gacatggaga
      721 ttccctcggc ctacgtgcag ttcctgcgca gcctgatgcc gcgcaagccg cgcgccgagc
      781 tgtacaacgc gctcggactg gccaagcagg gcaagaacct gttctaccgc gacctgctgt
      841 ggcacctgcg gcactcgagc gaccagttcc gcatcgcgcc cggcatcaag ggcatggtga
      901 tgctggtgtt cgacctgccc agctttccct acgtgttcaa gctcattcgc gaccgctttg
      961 ccgcacccaa ggaaaccacg cgcgagcagg tgcgcgccaa gtaccagctg gtcaagcgcc
     1021 acgaccgcgt gggacgcatg gccgacacca tggagttctc gctgctggcc tttccgcgcc
     1081 agcgtttcac cgacgagctg gtggccgagc tggagcagca tgccgccagc ctgctcgaaa
     1141 tctccgaccg cgacggcgac ggcgagaccg aagtcatcat cgcgcacgcc tacatcgagc
     1201 ggcgcatgat cccgctcaac ctgtacctgc aggaggcgct ggacgccggc gccggcgatg
     1261 ccgccgccag cgcgcaactg gagcacgccg tcatcgagta cggcaacgcc atcaaggacc
     1321 tggtggcggc caacatcttc cccggcgaca tgctgtggaa gaacttcggc gtcacccgcc
     1381 atggcaaggt ggtgttctac gactacgacg agatcgagta cgtcaccgac tgccagttcc
     1441 gccgcgtgcc gccgccgcgc cacgaggaag acgagctgtc cggcgaggtc tggtaccagg
     1501 tggcccgcaa cgacgtgttt cccgaaacct tcgcgccctt tctgctcggc cacccgcgcg
     1561 tgcgcgaggt cttcctgcgc caccacgccg acctgctgac gcccgagttc tggcagcaga
     1621 acaaggaacg catccagcgc ggcgaggtga tcgacttctt cccctacggc acgcaccggc
     1681 gcttcgactg caacggcggc gtcgccggcg cgcccgagct ggccgacccg ctggcggcgc
     1741 accacgaaac cccgtccggt ccgcccgatg ccctggcacc cctggcgccg gtgcccgaat
     1801 gaacgcctga actgcgccct gccatcccgt ttctgcttga aaggaaaaca gccatgtccg
     1861 accccatcgt catcgtttcc gccgcccgca cgcccatggg cagttttcag ggcgatttct
     1921 cgtcgctggc cgcgcacgac ctgggcggcg ccgccatccg ggccgccatc gagcgtgcgg
     1981 gcatccagcc ggaactcgtc accgaggtgc tgttcggcaa ctgcctgctg gccggccagg
     2041 gtcaggcgcc ggcgcgccag gccgcgttca agggcgggct gcccaagagc gcgggggcgg
     2101 tgacgctgtc caagatgtgc ggctcgggca tgcgtgcggc catgtttgcg cacgacatgc
     2161 tggtggccgg cagtcacgat gtgctggtgg ccggcggcat ggagagcatg accaatgccc
     2221 cgtatctgct gcaaaaaggg cgcgggggct atcgcatggg gcacgacaag gtgtacgacc
     2281 acatgatgct cgacggcctg gaggacgcct acgagcccgg ccgcagcatg ggcaccttcg
     2341 gcgaggactg cgccgccagg taccacttca cgcgcgagca gcaggacgcc tttgccaccg
     2401 ccagcgtgcg gcgcgcgcag gccgccatcg agagcggtgc cttccaagcc gagatcacgc
     2461 cggtcacggt caaggagcgc aagggcgagc gcgtggtcga caccgacgaa gggccgggcc
     2521 gcatcaagct cgacaacatc cccaagctca agcccgcctt caagaaagac ggcacggtga
     2581 cggcggccag cagctccagc atcaacgacg gtgctgccgc catggtgctg atgcgcgagt
     2641 ccaccgccag gaagctcggc tgcaggccgc tggcgcgcat tgccagccac gccacgcacg
     2701 cacaggagcc cgagtggttt tccaccgcgc cgatcggcgc caccgaaaag gcgctggcca
     2761 aggccggctg gcaggtgggc gacgtcgatc tgtgggaaat caacgaggcc tttgccgtgg
     2821 tgccgatggc gctgatgcac gatctgaagg tgccgcacga gaaggtcaac gtcaacggcg
     2881 gcgcctgcgc gctgggccac ccgatcggcg ccagcggcgc acgcatcatg gtgacgctga
     2941 tccacgccct gaaggcgcgc ggcctgaaga agggcctggc cacgctgtgc atcggcggtg
     3001 gcgaggcgac cgccgtggca ctcgaaatgc tatgaatagc atagctgttg gcgcacaaag
     3061 gacgggcgcc aaaggccttt tgatctgtat tccgatcgga gtcgcccgat gatcgattcg
     3121 ctcgacgcgc tgcgtgcgct ctacccggag gccagcgccc ggtcactggc caagcagctc
     3181 gaccggctgg acgaggccat gtgccgcttc gtcgcccatg cgccgctgtg cgtggtggcc
     3241 agcgtggggg tcggcggcgc actggccgac gcgtcgccac gcggtggtct gccaggcttc
     3301 gtgcgagtgc cggatgcgcg cacgctctgg atccccgacg ccaccggcaa caaccgcctg
     3361 gacacgctgg agaacctgct ggccgacccg cgcatcggcc tgctgtttct ggtgccgggg
     3421 gtggacgaag tgctgcgcgt caacggcacg gcgcgcctgc gcgacgaaga cgtcttcacg
     3481 cagcgctttg ccgccgaggg cgtgaaggcg ccgcggctgg tgatcgaagt cgccgtgcgc
     3541 gaggcctacc tgcactgccc caaggcgctg ctgcgggcgc gcctgtggtc gcctgaggtg
     3601 cgcgcggcgc ccggcacctt cccttccatg aacgccatgc tgcgcgccca gctgggcagc
     3661 gcggtgatca ccgaaaccga cgaacaggcg cgcgaacgct accgcgccca gctggccgac
     3721 gaaggcctgg cgcaccacgt gcgctgaccg ccgaacacca tgcccaccac cctgattctt
     3781 ggcgcctcgc gcggcatcgg cctggagctg gcacgccagt cgctggccgc cggcgagcgc
     3841 gtgatcgcca ccgcccgctc cgacgccggc ctgcaaacct tgcgcgcgct gggggcacag
     3901 gcactgcggc tggacgtggc ggagccggcc agcgtcagcg gcctggcctg gcagctcgac
     3961 ggcgagcaga tcgacagcgc ctggtacgtg gccggcgtga tgtgcgcgcg cgccgatgcg
     4021 cgccagccgc ccacggcacc ggcgttcgac cgcgtgatgc acaccaacgt gctgggcgcg
     4081 atgcaggtca tcccgcaggt ggcgccgctg gtggaagcgg cgggcgggcg cttcggtttc
     4141 atctccagca ccatgggccg catcggcgac gtcgaggcca gcaacgcctg gctgtaccgc
     4201 gtcagcaagg cggcgctgaa catggtggtg gcctcggcgc gccacagcta cccgcgggcg
     4261 gtgctggcgg cgtttcatcc cggctgggtg cagaccgaca tgggcggcgc ccaggcaccg
     4321 gtgacggtga ccgacagcgt ggccggtctg cgccgcacgc tggcggcgct cacgctggcc
     4381 gacagcggcg ccttcctgcg ctacgacggg cagcggctgg cgtcgtggtg aaacggcatg
     4441 acccatgagc cgcgtggcga tcgacgcgcc gaagggcgcc attcgtcatg catcctgccg
     4501 ccgtcaccgc agcggcacga agcggtactc gccgtgcgtg ccgagctcaa gctcgtcgcc
     4561 gcgccgccgc acgctgtgca tgctgccgtt caccgggatc accatcaggc cgtggtcggc
     4621 cagctgatcg accagcgcct gcggcaggcg gtcggcctcg gccgacacca ggatgcgatc
     4681 gaacggggcc agcgccggca ggccgagctg gcccggctcg gcggccacga tgcgggccca
     4741 gggctggccg gtggcggcca ggttgtcggc gccaaagcgc accagttcgg gcacccgctc
     4801 caccccatgc acctggccct ggggcccgac caggtgcgcc agcagggccg tggtccagcc
     4861 ggaaccggca cccacatcca acacccgcat gccgggtgcg acctgcagca gccgcagcat
     4921 gttggccacc gtgctgggct gggaattggt ttggccccag ccgatcggca gcgggcgatc
     4981 cagcgcggcc agggggcgct ggtcgagcgg cagaaaaccc tcgcgcggct gggccgcgaa
     5041 ggcggcgtcc accgggtcgg gcgcgggcgt catggccccg gcgtcgatgg cggctgccgg
     5101 ctcagcggcg gcgctgccca ccgccgccgg tgggcatggg gccgcggcgc tccaggtgac
     5161 ggaagctgat gcggcccttg gtgaggtcgt agggcgacag ctccagcgac acccggtcac
     5221 ccgccaggat gcggatgtgg ttcttcttca tcttgccggc ggaataggcc accagctcgt
     5281 ggccgttgtc cagcgtgacg cgaaagcgcg tgtccggcag cacttcgttg acgatgcctt
     5341 gcatctcgat cagttcttcc ttggccatgc gttgctccta aaacatgatg cggtgtgggc
     5401 accgccgggc atgcccgatg ggcgtgccgc gcggcgcgca gggttcaggt tcgccgggcg
     5461 tcaaagtggc cgtgaggcgc gggctcggac gcggcacctg atcagccgtt ccgttgccac
     5521 tgggtgcagg tgcgctggca agaccaggat ccgaagatgg cgccgacagg acccggcttg
     5581 ggcgccatcc acccggtcgc gcacgtgcaa ctcctggggt tagccggcaa ttgtatccgg
     5641 cgcagggttt ttctgccgcg ctttctgcgg tggcacgccg cgcccgggcg gccgcagggt
     5701 ggtgctgcga ccaccggtgg cgccgcacca ggtgtcgggc aggtgtaaag tgagtgtgca
     5761 ttcacaccct tacaactcga acacagaagc catgtttgta agcctggatg gcgtcagact
     5821 ggcagggtgt tcaagttcgg aaaggttgtt tccatgaccc cacgcagact ctcgcgcggc
     5881 gccgccgtcg tgctcatgac caccgccctg gcctggaacc tgccggcggc ggcgcagatc
     5941 gcgccggcct ccgacggcgg cgccaccgcc gtcacatcca cgcccgaggc tgcgcgtgcg
     6001 agcgtgccgc cagcggcggc ggcgggccag ccaggcccgg gcgagcgcgc acgcggcggc
     6061 acctttggcg gcggccgcgc ggcctcggcg gccgccgacc cggtggcgcc agcgccgacc
     6121 ccgcccgcct caggcccggc cacgggcgca gacgtaccgc cgccgcccgc catccacgcg
     6181 ccaggcaaat cctgaaggcc cactcgccgg ccaccggcac ccgttgttga cgcgaatcgg
     6241 ggcaagcgcc gatcgctatt caaaacgtag caatcgcgcc ctcacggggc gcgcctggcg
     6301 tccgccgctc gggggctggc cgggcggcgc gtggcgctcg cctgatcggc tgccggcacg
     6361 gcgggggcgc cgcattcggc ggcgcgctgg cgggcgcgca gtccgctcag ccgggtcgac
     6421 agatggtcca gcaccggcgg tggcagcggc tgctggaact cgaactccag ccgggcgatt
     6481 tcagccgcca ggtaggcgca ggcggcgtcg cgctggctgc ggctggcgcc ttccaggctc
     6541 agctccgccg gctggaccgg cggcacgctg tcgccggcca acccgaccac gccgttgggg
     6601 tcggccccca gcgcgggcgc gggcaggtct tcggtgccag gcgcgcaggg caggttggtg
     6661 taggtcagct gaccgtcgat gcggcagcga tgaatggcct ggcccaccgc ggccggtgcc
     6721 gcagcttcgg gcggcacgcc ggcctgcggc gcggtgacgg tgcgaccgag gggcgccggt
     6781 gccgccgatg ccgccaccac cgcgggcgcg gcggaggctg gcggcggcgc ctcggccacg
     6841 gcacgccgac gctcgacgtc ggtcaccagc cactggtagg cacgccaggc gccgccgacg
     6901 atcaccagca ccagcacggc cgccagcagc cagctgccca ggcgctgcgg ctcggcctcg
     6961 ggcggccgca gggacggttc ttcggcggga tcggcaggca cggtcatggc acggcaggag
     7021 cgggcgacgg cccgcgcgac cagggatgat acgggagccg gcacagggtc gtgcgggcgc
     7081 cgcgcgcagg cgttggccca ccctttgaat gctattcttt taatagctgt tggcgctgat
     7141 tggaaaagcg ccagaggccg aaaaggcctt cagtccggct ttttctcggg cagttcgatc
     7201 ttgacctcca gcacctccag gtcgtcctgg cgttccaggt gcaccttgat gtccttgggg
     7261 tcgatcctga cgtatttgct gatgaccgaa atcaggtcgc gctgcaggtc gggcaggtaa
     7321 tccggctcgg cggcactgcg gccggcgcgc tcgtgcgcca ggatgatctg cagccgctcc
     7381 ttggcgacgc tggcggtctt cttcttctcg cccagaaaga atgacaggaa ggacatggct
     7441 tcagcgcccc ccgaacaggc gcttgaagaa gccaggtttc tcggcctcga cgtagcgcat
     7501 cggcacctcg gcgcccagaa agcgctcgat cacgtccatg taggcaccgg caatgtcgct
     7561 gcccttcagg tgcaccgccg gcaggccctg gttggaggcc tgcagcacgg cttcgctctc
     7621 gggaatgacg ccgatcagct tgacgcgcag gatctcctgg atgtcgtcca ggctcagcat
     7681 ctggccgtcg gcgacgcggt tcgggttgta gcgcgtaatc agcaggtgct ccttgacggg
     7741 gtccttgccc tcgatggcac gctgcgtctt gctgcccagc atgcccagga tgcggtcgga
     7801 gtcgcgcacg ctggagacct cggggttggt gacgatcagc gcctcgtcgg caaagtgcat
     7861 ggccagcagc gcgccggtct cgatgccggc gggcgagtcg cacacgatgt agtcgaagtc
     7921 catcgccgcc aggtccttga gcaccttctc gacgccgtcc ttggtcagcg cgtccttgtc
     7981 gcgcgtctgg ctggcggcca gcacgaacag gttgtcgcac tgcttgtcct tgatgagggc
     8041 ctgcgtcagg ttggcctcgc cgtgaatcac gttgacgaag tcgtacacca cgcggcgctc
     8101 gcagcccatg atgaggtcga ggttgcgcag gccgacgtcg aagtcgatca ccgccgtctt
     8161 gtgccccttg agcgccagcc cggacgcgaa cgcggcgctg gtggtggtct tgcccacgcc
     8221 acctttgccc gaagtcacca ccacgattct tgccatgtcg atctcgcttg caaaatgaaa
     8281 accgtcgtcg ccgcgcgcct tcagcccgcc acgggttcga aaatcagctt gtcgccgacc
     8341 tcgccgctgg ccaggcgcac gctggcgcgc tggccccaca cggaaggcgg caggggttcg
     8401 tcgccggtgc ggtagacgcc ggcgatcgac accaggtcgg gccgcatctc gagcgcgaac
     8461 acgcgcgcct cggcccagcc gcgcgcgccg gcgatggcgc gcccgcgcag cggcgcatag
     8521 acgtggatgt ggccgtcggc aatcacctct gcccccgcgt tgaccatggc cagcagcacc
     8581 aggtcgcgcc cgcgcgcgta cacctgctgg cccgagcgca gcggccgctc gatcaccagc
     8641 gcgccgggcg gcggcggctc gacggcggcg ctgtcggcac ggcgcgccgg cgcggcatcg
     8701 ggcgccgccg cctgcgccac ccgcaggtcg ggcgcctgca gcaggccgat ggcgcgcgcc
     8761 gcctcatgct gcgcggcgct gccgccacgc accgccagca ggcgcagacg gcgcgcctgc
     8821 agcagctcgc ccagctgctg gaagtcgatc tcggcccccg cctgctcggc cggcagggtg
     8881 gacagatcga gcaccacggc gtcgtcctcg aagaagtcgg gccggtcgcc gtactgcgcc
     8941 agcagctcct cggccagccg cgccagatcg ggcgacttca ggcgcagcgt cagcagcggc
     9001 aggctggcgc tcttgatgtc gaaggtggtg gggctgcggc cggcgacggc aacggacatg
     9061 gcgggcggtc aaaaacgttc ccgcaggcgg gacgcaggga agcggggcga tcttaacagt
     9121 cgccagcggc gtgaacggcg ccgggccggg catcgacggg gcggagttgt gccggtttgt
     9181 ttttcttgag tcttttgaat tcgtagtagt cgtagtaggt gccctgccgt tttgtggaca
     9241 gcacgggttt ccctagtcct ggtgcgcact tgcagcgcct gcgcggctgt gcacaagcgg
     9301 ccgcggccgt ggtgcggccg tgggtacaag cggtcagggt cgttgccgcc tgtggataaa
     9361 cagatggttt tacaagaaac atccacagac ttgctccagt cgatcctgtg tgatgctggc
     9421 tcatctttgc acgtcgggcc aggaaagcac ttggccaacc ctcttttcct tttgggatga
     9481 ccctggcagg ggcatgatca gcatcgtatt gccgtttttc cggaaacttg cggactttcg
     9541 cagggtgttg ccgctcaacc gaccgtactt cgcccggccc tgggtcgagg tggtggtggt
     9601 gatggatgag aacgagtcgg aatcgggtgt gctcgacctg atccgccaat accccgacat
     9661 tcgctggcag gtgagggtga acgaccagcc tcatggatgg caaccgccct gcaaggcgat
     9721 caacgtcggg gtgaggcacg cgtcgcacga ggtggtgctg gtctgttcgc ccgaatcgat
     9781 gttcgtgacc gatgtgccgg cacaggcgct gggcacggtt cgggcggtgc cacgcggggt
     9841 ggcgctgggg cgtgtgggct ttggacagta cgcgcagctg catcagctgg gggcgtcggc
     9901 cgctttcgac gcggtggctc ccgatgcccg cgatccgttc aagctctacg gttcgatcgc
     9961 gctggcgcgg caggaactgg a
//
LOCUS       DAOOOG010000093         9887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_7883_length_9887_cov_26.721827, whole genome shotgun sequence.
ACCESSION   DAOOOG010000093 DAOOOG010000000
VERSION     DAOOOG010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7883_length_9887_cov_26.721827"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PK242_08125"
     CDS             complement(<1..590)
                     /locus_tag="PK242_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94023.1"
                     /translation="MASPKTVYTCHACGASSPRWLGKCPGCGAWNSLVEAVAEAATGG
                     RNRLGGAFAALAPSTAVRPLAAIEAVDVARSPTGQAELDRVLGGGLVAGGVVLIGGDP
                     GIGKSTLLLQALDSLQRAHPSTAGPPQGASAPSGGSATGAAVERGGRDPSTAGPPEGA
                     SAPSGGSATGAAVERGGRNPSTAGPPQGASAPSGGSA"
     gene            660..1886
                     /locus_tag="PK242_08130"
     CDS             660..1886
                     /locus_tag="PK242_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ94024.1"
                     /translation="MTTATAAPSPVSRNRLLVTSGLGWMFDAMDVGLLSFILAALKAD
                     WGLSAGQMGWLGAINSIGMAVGAIGFGMLADRIGRKQVFILTLLLFAIASGLSAFAST
                     LAVLFVLRFFIGMGLGGELPVASTYVSERVAAHERGRVVVLLESFWAVGWILAALIAY
                     FVIPRFAQDMGWRVALVLGALPAFYALYLRRQLPGDAPTAPPAAGLPWTARLAALWSP
                     AHARATLMLWLLWFTVVFSYYGMFLWLPSVMVGKGFALIKSFEYVLLMTLAQLPGYFT
                     AAWLIERAGRKFVLVVYLTGTAASAWAFGNADTQGALLLYGALLSFFNLGAWGALYAY
                     SPENYPAPIRASGVGTAAGIGRLGGILGPLTIPFLGAQGWGTGAIFGLFAATLLVGVL
                     AVAVLGRETRGAQVHA"
     gene            2086..3246
                     /locus_tag="PK242_08135"
     CDS             2086..3246
                     /locus_tag="PK242_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOZ94025.1"
                     /translation="MSFVLNRRRFSAGLALAPLATGGALSGCSQIPDRVKIGVAQPLT
                     GPIHELGKDLLNGVQLAVTELNAANYTINGKRVTLEVVAVDDKSDAETGKAVAQQLVE
                     AGVVAVIGHLNSGVSIAAAPIYAAQGMAQIAISTNPKFTELGLDTTFRMVANDHMQAK
                     AMGSYAGAQFGDVPYAAVDDGTPYGKGLIEGAARELKARKREIVLQQSFDDKTVAFDD
                     LAARIKAAKVGVIVSTLNDFQVIALIEALRKVNHTDVRMLGGDTLKTTGMLKARGTIR
                     GIHATSPVLEAKEFLSGRAFLSRYTSAFKIAPAYGGHYTYDSMHVLADAIQQAKSVKP
                     RDVTAVLRRISGSAPVTGSMRWDDKGEQRYAAVGVYDLGRDGWNLRIRSDTW"
     gene            complement(3269..4015)
                     /locus_tag="PK242_08140"
     CDS             complement(3269..4015)
                     /locus_tag="PK242_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOZ94026.1"
                     /translation="MATRYKYLTARNTALLGALLEGLRNAGYLRIYGWIGAGILLVTL
                     LLTLFDRHFDPQFPRRWRDGLAESFYAAVKIATAGQVPGRTNRLGWLGRVWQGVWLLP
                     AIALVAFVTSSVTSVLTTQSLHHQINSLQDVRDQPVGVLAGTVEEDYGRTQGLTLRSY
                     DDLDQASAALLAGEVQAVMADAPMLEYHAHTHPELPVKVVGRLFNRDRYGFALPLNSP
                     RTRDITLEVLKAVESDQVEQLRLRYFGRNP"
     gene            complement(4206..4634)
                     /locus_tag="PK242_08145"
     CDS             complement(4206..4634)
                     /locus_tag="PK242_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOZ94027.1"
                     /translation="MISFTQHPIPLRWRWLLCLALSPAVRAAAAATPASPLVVGIFES
                     PPFVMTAHERPSGLAIDLWEDIADRGQIAYRYRAYPSMRALLDAVQAQQVDLAVTSLT
                     INEQRARQMDFSQPWFEGGVRLLVSRQHRSSAVFRAVRRL"
     gene            complement(4689..5288)
                     /locus_tag="PK242_08150"
     CDS             complement(4689..5288)
                     /locus_tag="PK242_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94028.1"
                     /translation="MPSTAVLSHDIYPHIRVVLGMVIGLGVTRLLSGLARLVQHPGQH
                     RMDAVHLGWVGSTLLALVHFWWWQFGLYQIEAWTFGTYLFVIGYAIALFLLAALLFPD
                     SMAGYEGYGDYFQERRAWFFGVLASTYLLDVIDTLLKGDAHFARFAREYLIRTPILVA
                     LCMVAARTARRRFQVAFVALVLIYQLSWIFRLFNTLQQA"
     gene            complement(5340..5780)
                     /locus_tag="PK242_08155"
     CDS             complement(5340..5780)
                     /locus_tag="PK242_08155"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HOZ94029.1"
                     /translation="MTRTNRVLFRLAAGLTTALALQAVHASDDPCFEKAQSQAALNQC
                     ASAAYQRADADLNRLYQLMTARLSTDDAARKRLVDAQRKWLQFRDAECAFQTVRTVGG
                     SVQPMNVSSCLTDLTRERVTDLQKHLDCARVMGEGGACAVPPRR"
     gene            complement(5826..6380)
                     /locus_tag="PK242_08160"
     CDS             complement(5826..6380)
                     /locus_tag="PK242_08160"
                     /inference="COORDINATES: protein motif:HMM:NF022664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HOZ94030.1"
                     /translation="MSRIAHLLSGIAFAATTLGLTTPAQAQQTIECRSNNYGYNECYA
                     GDLSSPQLIHQTSSSACIVNRSWGFNPRRGYIWVNEGCSGVFADVGGYHHGRGDTWDQ
                     GARRYDDRGHDAGAIVGGLVLGAMLGAAAGDDHDAHRHARDRRSEEIDTRPQFDRDGN
                     PNFDVHGNYQGCHGLGCQVDNPDR"
     gene            complement(6586..7632)
                     /locus_tag="PK242_08165"
     CDS             complement(6586..7632)
                     /locus_tag="PK242_08165"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOZ94031.1"
                     /translation="MTGQTLSSRLATRWPLLQAPMAGAQDSAMALAVSGAGGLGALPC
                     AMLAPDALRRELQTLAASGLPYNVNWFCHPPPAPDAAREAAWRAALKPYFDELGLDPE
                     AVPGGPGRAPFSREAAELMAEFRPPVVSFHFGLPAPELLTRVKSWGAFVLSSATTVRE
                     ALWLQEHGADAIIAQGLEAGGHRGHFLSDDLSEQMGTFALLPQVVAAVRVPVIAAGGI
                     ASAAGVRAAQALGAAGVQVGTAYLCADEALTTPAHRAALHSEAARHTVITNLVTGRPA
                     RGMVNRVMRELGPISPAPSAFPLATAAMAYLRAAAEKQGRHDFTHWWSGQNASGCRAA
                     PAADITRALAQGFA"
     gene            7686..8162
                     /gene="hpaR"
                     /locus_tag="PK242_08170"
     CDS             7686..8162
                     /gene="hpaR"
                     /locus_tag="PK242_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HOZ94032.1"
                     /translation="MPRPTPFVHRNLPLLLLRAREALMQHYRPGLRAHGLSDQQWRVL
                     RVLREHEGGLETGRLAQQAYILGPSLTGMLARMEKSGLVARQRCPDDARRSLVAATPD
                     GLALADALSDAIEAQYQDLAAHLGQARLNQLYTLLDELIALPSHADAEPVEALMEP"
     gene            8166..8954
                     /locus_tag="PK242_08175"
     CDS             8166..8954
                     /locus_tag="PK242_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOZ94033.1"
                     /translation="MNPDLPWQPAGTVYGPLLNLRREHALWAPRMGQAPYQAAPKAPV
                     LYVKTANTLAACGAAVAVPQPVWLGPTLGLVVGDHASNWPPARNQQAPVAIKTIVLGG
                     VAIAGCVLLNDLSLPHDSYYRPAIRFRNRDGFLVCGTPAAPLAPAQTAALQIEARVNG
                     ALVQTVDLSTLVRDAATLLADVSAFMTLQPGDVLMLGTDCLADGSRPLAQAGDRVEVS
                     APGFAPLVHTVVAESSAAGPPQGARAPSGGSAGAPAPSVGARSS"
     gene            8951..9727
                     /locus_tag="PK242_08180"
     CDS             8951..9727
                     /locus_tag="PK242_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOZ94034.1"
                     /translation="MKHARIAWAGAVHEAVESEGQLELLTPAFRGRRLSFDEVVWLPP
                     LAPVPRARTVLALGLNYADHARELAFKPPEEPLAFAKGAASLIGHRAHTVRPDGVQNM
                     HYECELAVVIGRSAKKVRKDDAYDYVRGYTVANDFAIRDYLENWYRPNLRVKNRDTCT
                     PIGPWLVDAADVPDPMKLRLSTHVNGQLTQQGTTADMVFDVPTLIEYFTSFMSLNPGD
                     VILTGTPDGVVDCPVGSVVVCEIEGLGALQTTMVSESKVA"
BASE COUNT         1496 a         3351 c         3491 g         1549 t
ORIGIN      
        1 gcgctgcccc ccgagggggc gctcgcacct tggggcggcc cggcggtgct cgggttacgg
       61 cccccacgct ccacggctgc gcctgttgcg ctgccccccg agggggcgct tgcaccttcg
      121 ggcggcccgg cggtgctcgg gtcacggccc ccacgctcca cggctgcgcc tgttgcgctg
      181 ccccccgagg gggcgctcgc accttggggc ggcccggcgg tgctcgggtg cgccctctga
      241 aggctgtcca gggcctgcag cagcagggtg gatttgccga tgccggggtc gccgccgatc
      301 agcaccacgc cgccggccac caggccgccg cccagcacgc ggtccagctc ggcttggccg
      361 gtggggctgc gcgccacgtc caccgcctcg atggcggcca gcggccgcac ggcggtcgat
      421 ggggccaatg ccgcgaatgc gccgcccaga cggtttctgc cgccggtggc ggcttcggcc
      481 accgcctcga ccaggctgtt ccaggcgccg cagccggggc atttgcccag ccagcgcgga
      541 ctgctggcgc cgcatgcgtg gcaggtgtag acggtcttgg ggctggccat ggggcaatcg
      601 taacggcgcc gggcgggtac catgcgccac cccaggcccg atcccgcttg catcgtctca
      661 tgaccaccgc caccgccgcg ccgtcgcccg tctcacgcaa ccgcctgctc gtcacttcgg
      721 gcctgggctg gatgttcgac gccatggatg tcggcctgtt gtcgttcatc ctggccgcgc
      781 tcaaggccga ctggggcctg tcggccggac agatgggctg gctgggcgcc atcaactcga
      841 tcggcatggc ggtgggggcg atcggcttcg gcatgctggc cgaccgcatc ggtcgcaagc
      901 aggtgttcat cctgacgctg ctgctgtttg ccatcgccag cggtctgtcg gcctttgcca
      961 gcacgctggc ggtgctgttc gtgctgcgct ttttcatcgg catgggcctg ggcggcgagc
     1021 tgccggtggc cagcacctac gtgtccgagc gcgtggccgc acacgagcgc ggccgcgtgg
     1081 tggtgctgtt ggagagcttc tgggccgtcg gctggatcct ggcggcgctg atcgcctact
     1141 tcgtcatccc gcgctttgcc caggacatgg gctggcgcgt ggccctggtg ctgggcgcgc
     1201 tgcccgcctt ctatgccctg tacctgcggc gccagctgcc cggcgatgcg cccaccgccc
     1261 cgccggcggc cggcctgccg tggacggcgc gtctggcggc gctgtggtcg cctgcgcatg
     1321 cgcgcgccac actgatgctg tggctgctgt ggttcaccgt cgtgttctcg tactacggca
     1381 tgtttctgtg gctgcccagc gtcatggtcg gcaagggctt tgcgctcatc aagagcttcg
     1441 agtacgtgct gctgatgacg ctggcgcagc tgcccggcta cttcaccgcc gcctggctga
     1501 tcgagcgcgc cgggcgcaag ttcgtgctgg tggtgtacct gaccggcacg gcggccagcg
     1561 cctgggcctt cggcaacgcc gacacgcagg gcgcgctgct gctgtatggc gcgctgctgt
     1621 cgttcttcaa cctgggcgcc tggggcgcgc tgtacgccta ttcgcccgaa aactacccgg
     1681 cgccgatccg cgccagcggc gttggcacgg cggccggcat cgggcgcctg ggcggcatcc
     1741 tggggccgct gaccatcccc ttcctggggg cgcagggctg gggcaccggc gccatcttcg
     1801 gcctgttcgc cgccaccttg ctggtgggcg tgctggcggt cgccgtgctg gggcgcgaga
     1861 cgcgcggcgc gcaggtacac gcctgacctg gcgggtctgc gccatggtgc cgttgtggtc
     1921 ggtccacaac gagccacgag tgaactaatt cacgcgtgat cttgcggcca catggcctgg
     1981 atggccatca tcaataatac ttttatattg ttgattcgat ctgcccgtgg cgcagcggag
     2041 cgcgggtggt gcgtgattct ggccagtctc atcaggagtg aagtcatgtc ctttgtcctc
     2101 aaccgtcgac ggttctccgc cggcctggca ctggcgccgc ttgccaccgg cggcgcgctg
     2161 agcggctgca gccagattcc cgatcgggtc aagatcggcg tggcacagcc gttgacaggg
     2221 ccgatccacg aactgggcaa ggacctgctc aacggggtgc agctggcggt gaccgagctg
     2281 aacgcggcca actacaccat caacggcaaa cgcgtgacgc tggaagtcgt tgccgtggac
     2341 gacaaatcgg acgccgagac cggcaaggcg gtggcgcagc agctggtgga ggccggcgtg
     2401 gtggccgtga tcggtcatct caactcgggc gtcagcatcg ctgccgcacc gatctacgct
     2461 gcccagggca tggcccagat cgccatttcc accaacccga agttcaccga gctgggcctg
     2521 gacaccacct tccgcatggt ggccaacgat cacatgcagg ccaaggccat gggttcctat
     2581 gcgggcgccc agttcggcga cgtgccctac gccgcggtcg acgacggcac cccctatggc
     2641 aaggggctga tcgaaggagc ggcgcgcgaa ctgaaggcaa gaaagcgcga gatcgtgctg
     2701 cagcagtcgt ttgacgacaa gacggtggcc ttcgatgacc tggcggcccg gatcaaggcg
     2761 gccaaggtgg gggtgatcgt ctccacgctc aacgacttcc aggtcatcgc gttgatcgag
     2821 gcgctgcgca aggtcaacca caccgatgtg cgcatgctgg gcggcgacac gctcaaaacc
     2881 accggcatgc tcaaggcgcg cgggacgatc cgcggcatcc acgccacatc gccggtgctg
     2941 gaggccaagg agtttctgtc gggacgggca tttctgagcc gctacaccag cgcgttcaag
     3001 attgctccgg cctatggcgg gcactacacc tacgacagca tgcacgtgct ggccgatgcc
     3061 atccaacagg ccaagtcggt caagccgcgc gacgtgacgg ccgtgctgcg ccgcatcagc
     3121 ggctcagccc cggtcaccgg atccatgcgc tgggacgaca agggcgagca gcgctacgct
     3181 gccgtcggtg tctacgacct ggggcgcgac ggctggaacc tgcgcatccg ctcggacacc
     3241 tggtagcgcg gccggggccg ccgctcggtc acgggttgcg accgaagtag cgcagcctca
     3301 gctgttcgac ctggtccgat tcgaccgcct tcagcacctc cagcgtgatg tcgcgcgtgc
     3361 gtgggctgtt cagcggcagg gcaaagccat agcggtcgcg gttgaacagg cggcccacca
     3421 ccttgaccgg cagttccggg tgggtgtggg cgtggtactc gagcatcggc gcatcggcca
     3481 tgaccgcctg cacctcgccc gccagcaggg ctgcgctggc ctgatcgagg tcgtcgtagc
     3541 tgcgcaacgt caggccctgc gtgcggccgt agtcctcctc cacggtgccg gcgagcacgc
     3601 ccacgggttg atctctcaca tcctgcaggc tgttgatctg gtggtgcagc gactgggtgg
     3661 tcagcacgct ggtgaccgat gacgtgacga aagccaccag ggcgatggcc ggcagcagcc
     3721 acacgccctg ccagacgcgg cccagccagc ccaggcggtt ggttcgccca ggcacctggc
     3781 cggcggtggc gattttcacg gcagcgtaga agctctcggc caggccgtcg cgccagcggc
     3841 gcggaaactg cgggtcgaag tgccggtcga acaatgtcag cagcagcgtg accagcagaa
     3901 tgccggcccc gatccagcca tagatcctca ggtagccggc gttgcgcagg ccttccagca
     3961 gggcaccgag tagtgcagtg tttcgcgctg tcaggtactt atagcgagtc gccaaggggc
     4021 cagctgcgcg gcgcgtggcc gcaggcatgg tggacccatg tcaaggccac gccacaaagc
     4081 agatggcccc ttggcggctc gccctgcggg aagccagaaa aaggcttgcg gcggcgttgc
     4141 gcctcttgcc aaggccgcca gcatgggctg cgagccgcgc cttgccgcaa gcctttttct
     4201 ggcttctata agcgcctgac agcgcgaaac actgcacttg accgatgctg gcggctcacc
     4261 agcagacgca cgccgccttc gaaccagggc tgggaaaagt ccatctgccg cgcccgctgt
     4321 tcgttgatgg tcaggctggt gacggccagg tccacctgct gcgcctgtac ggcgtcgagc
     4381 agggcgcgca tgctcgggta ggcgcggtac cggtaggcga tctggccgcg gtcggcaatg
     4441 tcttcccaca ggtcgatggc cagtccgctg ggccgctcgt gcgccgtcat cacgaaagga
     4501 ggggactcga agatgcccac gaccagcggc gatgccggcg tggctgccgc ggccgcccgc
     4561 acggcagggc tcagggccag gcagagcagc cagcgccagc gcaggggtat ggggtgctgg
     4621 gtgaaagaga tcatggcttg aaagggagca gatggccgcg gcgaggccgg cacggcgggc
     4681 gacgcctctc aggcttgctg cagtgtgttg aacagccgga agatccacga aagctgatag
     4741 atcagcacca gagcgacgaa ggcgacctga aagcgccgcc gagcggttct ggccgcaacc
     4801 atgcacaaag ccaccaggat cggcgtgcgg atcaggtatt cgcgcgcaaa gcgggcgaag
     4861 tgcgcgtcgc ccttgagcag cgtgtcgatg acgtccagca gataggtgct ggccagcacg
     4921 ccaaagaacc aggcgcggcg ctcctgaaag tagtcgccgt agccctcata gcccgccatg
     4981 ctgtccggaa acagcagcgc cgccagcaga aacagcgcga tggcgtagcc gatcacgaac
     5041 aggtaggtgc caaaggtcca ggcctcgatc tggtagagcc cgaactgcca ccaccagaag
     5101 tgcaccagcg ccagcagggt ggagccgacc cagcccaggt gcacggcatc catgcggtgc
     5161 tggcccggat gctgcaccag ccgcgccagg cccgacagca ggcgcgtgac ccccaggccg
     5221 atgaccatgc ccagcaccac ccggatgtgg gggtagatgt cgtgactcag gacggcggtc
     5281 gacggcatgc gcgcaacggc gggcggcccg gatgtgccgt cccgcgatgg gggcggactt
     5341 cagcgccgcg gcggcacggc gcaggcgccg ccttcaccca tgacgcgcgc gcagtccagg
     5401 tgcttttgca gatccgtcac ccgctcgcgc gtcagatcgg tcaggcaact gctgacattc
     5461 atgggctgga cgctgccgcc gaccgtgcgt acggtctgga acgcgcactc ggcatcgcga
     5521 aactgcagcc acttgcgctg cgcatcgacc aggcgcttgc gcgccgcatc gtcggtgctc
     5581 aggcgcgccg tcatgagctg gtagagccgg ttgaggtcgg cgtcggcgcg ctggtaggcg
     5641 gcgctggcgc actggttcag tgcggcctgc gactgggctt tttcgaagca ggggtcgtcc
     5701 gaggcgtgca ctgcttgcag cgccagtgcc gtggtcagtc cggcggccag gcgaaaaaga
     5761 actcggttgg ttcgggtcat gtggtttctc cgcaggtgtg gctgtggcgg gtgttcgggc
     5821 ggcgctcaac ggtcggggtt gtcgacctga cagcccaggc cgtggcagcc ctggtagttg
     5881 ccgtggacgt cgaaattggg gttgccgtcg cggtcgaact gcgggcgggt gtcgatctct
     5941 tccgagcggc ggtcgcgcgc atgccggtgc gcgtcgtgat cgtcgccggc cgctgcgccc
     6001 agcatggcgc ccaacaccag accgccgacg atggcgccgg cgtcgtgacc tcggtcgtcg
     6061 tagcggcgcg ccccctggtc ccaggtgtcg ccgcgtccat ggtggtagcc tccgacgtcg
     6121 gcaaacaccc cggagcaacc ttcattgacc cagatatagc cccggcgcgg gttgaacccc
     6181 cagctgcggt tgacgatgca ggcgctgctg gaggtctggt ggatcagttg cggggacgac
     6241 aggtcgcccg catagcactc gttgtaaccg tagttgttgg agcggcattc gatcgtttgc
     6301 tgcgcttggg cgggtgtggt caggcccagc gtcgtggcag cgaatgcgat gccgctcagc
     6361 agatgcgcga tgcgagacat gttcaagctc cttcaggggt gcgcccgggg ggcgcggtag
     6421 gcgatggcga cggcgttgca gtccaaagcg ccaacgcaag accggcgccg tcgttctcat
     6481 cgtaaaggaa cgcgtaccgg caggctatgc ggcaggtcca cgatcggtca tacgagatgc
     6541 gttatgccga gggcgggccc ggtgcccacc acggccgtgg cggggtcagg caaagccctg
     6601 cgccagggcg cgcgtgatgt cggccgccgg cgcggcccgg cagccgctgg cgttctggcc
     6661 gctccaccag tgggtgaagt cgtgccggcc ctgcttttcg gcggcggcgc gcagataggc
     6721 catggcggcc gtggccagcg gaaaggccga gggcgcgggg ctgatcggcc ccagctcgcg
     6781 catcacccgg ttgaccatgc cgcgcgccgg ccggccggtg accaggttgg tgatgacggt
     6841 gtggcgcgcc gcctcgctgt gcagcgcggc gcggtgcgcg ggcgtggtga gcgcctcgtc
     6901 ggcgcacagg taggccgtgc ccacctgcac gccggccgcg cccagcgcct gtgcggcgcg
     6961 cacgccggcg gcgctggcga tgccgccggc ggcgatcacc ggcacgcgca cggcggccac
     7021 cacctgcggc agcagggcga acgtgcccat ctgttcgctc aggtcgtcgg acagaaagtg
     7081 gccgcgatgg ccgccggctt ccagcccctg ggcgatgatg gcatcggcac cgtgctcctg
     7141 taaccatagc gcctcgcgca cggtggtggc cgatgaaagc acaaaagcgc cccaggattt
     7201 gacgcgcgtc agcagctcgg gcgcgggcag cccgaagtga aagctcacca ccggcgggcg
     7261 gaactccgcc atcagctcgg ccgcctcccg gctgaacggc gcgcggccgg ggccgccggg
     7321 cacggcttcc gggtccagcc ccagttcgtc gaaatagggt ttgagcgccg cgcgccaggc
     7381 cgcctcgcgc gccgcgtcag gcgccggcgg cgggtggcag aaccagttga cgttgtaggg
     7441 caggccgctg gcggccaggg tctgcagctc gcgccgcagc gcgtcgggcg ccagcatggc
     7501 gcagggcagg gcgcccaggc cgccggcgcc gctcaccgcc agcgccatgg cgctgtcctg
     7561 ggcgccggcc atgggcgcct gcagcagggg ccagcgggtg gcaaggcgcg aactcagggt
     7621 ttgtccggtc atgcgcagat tgtgaaccgc ttgcgtcacc ggtggaaatt taatatatta
     7681 aataaatgcc ccgcccgacg ccgttcgtcc accgcaacct gccgctgctg ctgctgcgcg
     7741 cgcgcgaggc actgatgcag cactaccgcc ccggcttgcg ggcgcacggc ctgtcggacc
     7801 agcaatggcg cgtgctgcgc gtgttgcgcg agcacgaggg cggcctggaa accgggcggc
     7861 tggcgcagca ggcctacatc ctgggcccca gcctgaccgg catgctggcg cgcatggaaa
     7921 agagcggtct ggtggcgcgc caacgctgtc cggacgacgc gcgccgcagc ctggtggccg
     7981 ccacgccgga cgggctggcg ctggccgatg cgctgtccga cgcgatcgag gcgcagtacc
     8041 aggacctggc ggcgcacctg gggcaggcgc gcctgaacca gctctacacc ctgctggacg
     8101 agttgatcgc cctgccttcc catgccgacg ccgagccggt cgaagcgctg atggagccat
     8161 gaacgatgaa ccctgacctg ccctggcagc ccgccggcac cgtgtacggc ccgttgctga
     8221 acctgcgacg cgaacacgcg ctgtgggcgc cgcgcatggg ccaggcgccg taccaggcgg
     8281 cgcccaaggc cccggtgctc tacgtcaaga ccgccaacac ccttgccgcc tgcggcgccg
     8341 cggtcgccgt gccgcagccg gtgtggctgg ggccgacgct cgggttggtg gtcggagatc
     8401 atgcatccaa ttggcctcca gcgcgtaatc agcaagcgcc ggtagctatc aaaaccatag
     8461 tcctcggagg tgtcgcgatc gccggctgcg tgctgctgaa cgacctcagc ctgccgcacg
     8521 acagctacta ccgcccggcc atccgttttc gcaaccgcga cggctttctg gtctgcggca
     8581 cacccgccgc gccgctggcg ccggcgcaga ccgccgcgct gcagatcgag gcgcgtgtca
     8641 atggcgcgct ggtgcagacc gtggatctgt ccaccctggt gcgcgacgca gccaccctgc
     8701 tggccgacgt cagcgccttc atgacgctgc agccgggtga cgtgctgatg ctgggcaccg
     8761 actgcctggc cgatggcagc cggccgctgg cgcaggcggg cgaccgcgtc gaggtcagcg
     8821 cgcccggctt tgcgccgctg gtgcataccg ttgttgcgga gtcgagcgcc gccgggccgc
     8881 cccaaggcgc gagggcaccc tcggggggca gcgccggcgc gccagcgcca agcgtggggg
     8941 cacgttcatc atgaaacacg cccgcatcgc ctgggccggc gccgtgcacg aggccgtcga
     9001 gtccgagggc cagctcgaac tgctgacgcc cgccttccgc ggccgccgcc tgtcctttga
     9061 cgaggtggtc tggctgccgc cgctggcgcc cgtgccgcgc gcgcgcaccg tgctggcgct
     9121 cggcctgaac tacgccgacc acgcgcgcga actcgccttc aagccgcccg aggagccgct
     9181 ggcctttgcc aagggcgcgg cctcgctgat cggccatcgc gcccacacgg tgcgacccga
     9241 cggcgtgcag aacatgcact acgaatgcga gctggcggtg gtgattggcc gcagcgcgaa
     9301 gaaggtgcgc aaggacgacg cctacgacta cgtgcgcggc tacaccgtgg ccaacgattt
     9361 cgccatccgc gactacctgg aaaactggta ccggcccaac ctgcgcgtca agaaccgcga
     9421 cacctgcacc cccatcggcc cctggctggt cgacgcggcc gacgtgcccg atcccatgaa
     9481 gttgcggctg agcacgcacg tcaacggcca gctgacccag cagggcacca ccgccgacat
     9541 ggtgttcgac gtgccgacgc tgatcgagta cttcacgagc ttcatgagct tgaacccggg
     9601 cgatgtgatc ctgaccggca cgcccgacgg cgtggtcgat tgcccggtgg gctcggtggt
     9661 ggtgtgcgag atcgaaggcc tgggcgcctt gcagaccacc atggtttctg aatcaaaagt
     9721 ggcctgagcg cccgtccacc aagtgagagc agctatgaaa attgatcatc tcatcgacgg
     9781 caagccggtt gccggcagcg actacttcga atccatcaac cccgctaccc aaggcgtgct
     9841 ggccgaggtg gccagcggcg gcgaggccga ggtcaacgcg gcggtgg
//
LOCUS       DAOOOG010000094         9803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_8000_length_9803_cov_22.846122, whole genome shotgun sequence.
ACCESSION   DAOOOG010000094 DAOOOG010000000
VERSION     DAOOOG010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9803
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8000_length_9803_cov_22.846122"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PK242_08185"
     CDS             complement(<1..926)
                     /locus_tag="PK242_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOZ94035.1"
                     /translation="MLLLAAALAGCSAPPPAPPPAVAAPPAPAVVQAPALPQADSGAA
                     FTDRLGMRFVRVPAGEFMMGSAESPQALAKAFPHAERRRLTDLADEQPVHRVRLTRDF
                     WLGAHEVTVGQFRQFVQASGYVPESVRDGSGAYGFNPRYDPSRTQRGDLFEGRDPRYS
                     WQNPGFAQTDAHPVVNVTWNDAVAMARWLSEREGVTYRLPTEAEWEYAARAGSRTRYP
                     GGDDPQVLLRSANTFDRETALRWPRWRDQAGHGSDGHAFTAPVGSYAPNAFGLYDMIG
                     NVWEWCADWYGEDYYRRSPTDDPAGPASGKVRV"
     gene            complement(1052..1936)
                     /locus_tag="PK242_08190"
     CDS             complement(1052..1936)
                     /locus_tag="PK242_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOZ94036.1"
                     /translation="MKHYFMHGFYNKPSPQPDRRRALCALVGGLTLTWAAQARADDGG
                     DWPRQAAVPGGVTRLELGPAPARPQAFADDAPLLVLGDPIAWSALVGIPLSAAPGTAH
                     IRVVTETGERRLPYSIAPRRYAEQHLKVAPRTVDLSPTDLARHERERAHQQGVIASFS
                     APPEGSLRMRAPCTGPRSDSFGKRRFFNGQPRNPHSGLDIAAPTGTPVLAPLAGAVID
                     TGDYFFNGQTVWLDHGGGLLSMLCHLSRIDCRPGEHLAAGQRLGAVGASGRVTGPHLH
                     WTVMLNRAAVNPALFLPA"
     gene            2092..2604
                     /locus_tag="PK242_08195"
     CDS             2092..2604
                     /locus_tag="PK242_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOZ94037.1"
                     /translation="MAYLHWVPELDTGIPEIDTQHKRILDYINKLHDLRDSPDRAALG
                     DVIGETVDYTLSHFAFEESVIESAGYMFAGPHKKVHELFTRKVAEMQSRFDAGEDVAA
                     ELHGMLSRWLFNHIRNEDHGYIDSVKAYLRMTSKQREADKAELKAELLQELAARQNGK
                     KGWFARLFGG"
     gene            complement(2636..3274)
                     /locus_tag="PK242_08200"
     CDS             complement(2636..3274)
                     /locus_tag="PK242_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOZ94038.1"
                     /translation="MNSAARLAPRALYEEVAERLRQRIFSGELAPGDWIDELKIADEL
                     GISRTPLREALKVLAAEGLVTMKVRRGAYVTETSGKDLADVYHLLALLETDAAQAVAL
                     HADAAGRAELAALHAELEAAVGDAARFFALNEAFHLRLLELADNRWRLQMVQDLRKVM
                     KLHRRHSLQKAGRLQASLAEHRALMQAIAARDGAAAAACMQAHFQSGLEAAS"
     gene            3517..5673
                     /gene="scpA"
                     /locus_tag="PK242_08205"
     CDS             3517..5673
                     /gene="scpA"
                     /locus_tag="PK242_08205"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOZ94039.1"
                     /translation="MSQDPASFTPATLADWRKAAAKSAPGGDLSALDWHTPDGITVKP
                     LYTAADVQGLPHADTLPGFEPFVRGPQATMYAVRPWTIRQYAGFSTAEESNAFYRKAL
                     AAGGQGVSVAFDLATHRGYDSDHPRVTGDVGKAGVAIDSVEDMKILFDGIPLDKVSVS
                     MTMNGAVLPVLAGYIVAAEEQGVRQDQLAGTIQNDILKEFMVRNTYIYPPGPSMRIIG
                     DIIEYTAQHMPKFNSISISGYHMQEAGATQALELAFTLADGKQYVRTALAKGMDVDAF
                     AGRLSFFWAIGMNFYLEIAKMRAARLLWCRIMKGFGAKNPKSLMLRTHCQTSGWSLTE
                     QDPYNNVVRTAIEAMAAVFGGTQSLHTNALDEAIALPTEFSARIARNTQLIIQEETHI
                     TSVVDPWAGSYMMESLTQQMADAAWAIIEEVDAMGGMTRAVDSGWAKLKIEAAAAEKQ
                     ARIDSGKDVIVGVNKYKLAREDAVDILEVDNVKVREAQIARLKDIKQKRDPAQVQQAL
                     AAITTIAQNGQGNLLAAAVDAIRARATVGEVSDAMEQVFGRHRADTQKVTGVYAAAYE
                     GAEGWDKLKQEIAGFAEAEGRRPRVMIAKLGQDGHDRGAKVVATAFADLGFDVDIGSL
                     FQTPEECARQAIENDVHAVGVSTLAAGHKTLVPAIINELKKQGADDIVVFVGGVVPRQ
                     DYDFLYDAGVKGIYGPGTPIPASAKDVLEQIRQARP"
     gene            5726..6745
                     /gene="meaB"
                     /locus_tag="PK242_08210"
     CDS             5726..6745
                     /gene="meaB"
                     /locus_tag="PK242_08210"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOZ94040.1"
                     /translation="MSPAVSAQAITQSRGPAQRRAIAKAITLLESTRADHRAQGDELL
                     TALLPHTGGALRLGISGVPGVGKSTFIEALGVYLVERGHRVAVLAIDPSSTVSGGSIL
                     GDKTRMERLSALEGAYIRPSPSSGTLGGVAEKTREAMLVCEAAGYDVVLVETVGVGQS
                     ETAVHGMTDMFCVLQLPNAGDDLQAIKKGVMELADLVVINKADLDPAAATRARAQIQS
                     ALRVFGQQGNPQHALQDASVWHPEVIQLSGLKGEGLDAFWDCVSRFRALQSAGGRLHE
                     RRRQQSLAWMWERIDAGLKAAFRAHPEVRQALPAMTQDVAQGRLPPSTAARRLLALVA
                     PESVA"
     gene            6742..7572
                     /locus_tag="PK242_08215"
     CDS             6742..7572
                     /locus_tag="PK242_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94041.1"
                     /translation="MTAMALSPQDLQRIAQSPGWRGGRVEVCDTSQGKVIVKGWRPAH
                     HPARYGLLNRLARWLGLPFLRAVPLAGGAQAQHTEVARLRALRRAGARVPEVLHVGSD
                     HFVMQWLGRAHLGDVLQSGHPGAAALWREAGDELVRLHRAGQYLSQGFARNMIVTDEA
                     GDTPRLAGLIDFEDDPLQVMGLADAQVRDWLAFLHSTLWTLPLAPSEVDACIDAWLDA
                     ESPAVRAGFAQACRRLAWLRVLPRQRRFGRDTVALQAAAAAAHRHARRHPISLDNRSN
                     "
     gene            7582..9114
                     /locus_tag="PK242_08220"
     CDS             7582..9114
                     /locus_tag="PK242_08220"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOZ94042.1"
                     /translation="MKPILEQLEQKRVAARLGGGQKRIDAQHAKGKLTARERIEVLLD
                     AGSFEEWDMFVEHRCADFGMDATHIPGDGVVTGYGTINGRLVFVFSQDFTVFGGALSE
                     AHAEKICKVMDQAMKVGAPVIGLNDSGGARIQEGVASLGGYAEVFQRNVMASGVIPQI
                     SMIMGPCAGGAVYSPAMTDFIFMVKDTSYMFVTGPEVVKTVTHESVTSEQLGGALTHT
                     TRSGVADLAFENDVEALLMLRRFFNYVPASNREKAPYRPTQDPADRADLSLDTLVPDN
                     PNLPYDIKEAIVKTVDDGEFFELQPDYAANIVIGFGRMDGHVVGIVANQPLVLAGCLD
                     IKSSIKAARFVRFCDAFNIPILTFVDVPGFMPGTSQEYGGIIKHGAKLLYAYAECTVP
                     KITVITRKAYGGAYDVMSSKHLRGDVNLAWPNAEIAVMGAKGAVEIIFREEKKDPAKL
                     AAREAEYKARFANPFVAGARGFIDDVILPHETRQRICRSLAMLKDKKLDNPWRKHGNI
                     PL"
     gene            9135..9428
                     /locus_tag="PK242_08225"
     CDS             9135..9428
                     /locus_tag="PK242_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOZ94043.1"
                     /translation="MPKAANAQLARQLTDIPNIGPSIASDLRLLGIRSPADVRAMDPL
                     AAYDALRTPMGHRHDPCVLDTFLAAHDFMHGGQARPWWHFTARRKALLASLKR"
     gene            9572..>9803
                     /locus_tag="PK242_08230"
     CDS             9572..>9803
                     /locus_tag="PK242_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOZ94044.1"
                     /translation="MFTKILIANRGEIACRVIATARKMGIATVAVYSDADAQARFVQM
                     ADEAVRLGPPPSRESYLLGDKIIEAAKATGAQA"
BASE COUNT         1647 a         3387 c         3381 g         1388 t
ORIGIN      
        1 acccgcacct tgccgctggc cgggccggcg gggtcgtcgg tgggcgagcg gcggtagtag
       61 tcctcgccgt accagtcggc gcaccactcc cacacgttgc cgatcatgtc gtacagaccg
      121 aaggcgttgg gcgcatagct gccgaccggg gcggtgaagg cgtggccgtc gctgccgtgg
      181 cccgcctggt cacgccagcg cggccagcgc agcgcggttt cgcggtcgaa ggtgttggcg
      241 ctgcgcagca gcacctgcgg gtcgtcgccg cccgggtagc gcgtgcggct gccggcgcgg
      301 gcggcgtatt cccattcggc ctcggtcggc aggcggtagg tcacgccctc gcgctcgctc
      361 agccagcgcg ccatggccac ggcgtcgttc caggtgacgt tgaccaccgg gtgcgcgtcg
      421 gtctgggcaa agcccgggtt ctgccacgaa tagcgcgggt cgcgcccttc gaacagatcg
      481 ccgcgctggg tgcgcgacgg gtcgtagcgc gggttgaagc cgtaggcgcc gctgccgtcg
      541 cgcaccgact cgggcacgta gcccgaggcc tgcacgaact ggcggaactg ccccaccgtg
      601 acctcatgcg cgccgagcca gaagtcgcgc gtcagacgca cccgatgcac cggttgttcg
      661 tccgccagat cggtcaggcg gcgccgctcc gcgtgcggaa aggccttggc cagcgcctgc
      721 ggcgactcgg cgctgcccat catgaattcg cccgccggca cgcgcacgaa gcgcatgccc
      781 aggcggtcgg tgaaggcggc gccgctgtcg gcctgcggca gcgccggcgc ctgcaccacg
      841 gccggggcgg ggggcgcggc gaccgccggc ggcggcgcgg gcggcggtgc gctgcatccg
      901 gccagcgccg cggcaagcag cagcatcagg gcggttgggg cggatgacga tcggggggtg
      961 ggcaggggtc ggcgcatggc ggcccatgct tgcagtgcgc cgtcggcgcg tcaatgcgga
     1021 ggaatcccga tgtggcgccg ggggtggcct gtcaggcggg caggaacagc gccgggttga
     1081 ccgcggcgcg gttgagcatc accgtccagt gcaggtgcgg cccggtcacg cgaccgctgg
     1141 caccgacggc gcccaggcgc tggccggcgg ccaggtgctc gcccggccgg cagtcgatgc
     1201 ggctgaggtg acacagcatc gacagcaggc caccgccatg gtccagccag acggtctggc
     1261 cgttgaagaa gtagtcgccg gtatcgatca ccgcgccggc cagcggcgcc agcaccggcg
     1321 tgccggtggg cgcggcgatg tccaggccgc tgtgcgggtt gcgcggctgg ccattgaaga
     1381 aacgccgctt gccgaacgaa tcggagcgcg gtccagtgca gggcgcgcgc atgcgcaggc
     1441 tgccttcggg cggcgcgctg aagctggcga tcacgccttg ctggtgcgcg cgctcgcgtt
     1501 cgtggcgcgc cagatcggtg ggtgacaggt ccaccgtgcg cggtgccacc ttcaggtgct
     1561 gctcggcata gcgccgcggg gcgatgctgt agggcaggcg acgctccccg gtttcggtga
     1621 cgacgcggat gtgcgccgtg ccgggcgcgg ccgacagcgg aatgccgacc agggcgctcc
     1681 aggcgatggg gtcgcccagc accagcagcg gtgcgtcgtc ggcgaaggct tgcggtcgag
     1741 cgggtgccgg gcccagctcc aggcgtgtca cgccgcctgg cacggcggcc tggcgtggcc
     1801 agtcgccgcc atcgtcggct cgcgcctgcg cggcccaggt gagggtcaga ccgcctacca
     1861 aagcacacag cgcacggcgc cgatcgggtt gcggagacgg tttgttgtaa aaaccatgca
     1921 tgaagtagtg tttcatgggt ttggaatgga ccagtgtgcg ccgaatgcta tgaaagcagg
     1981 agcatatcga gcctcggttg gtaacagatc ttgactgctt gactcaaatc aaaagtattc
     2041 atgccgggtc cctgaatact gggcaccatt ccccaacaga gactgattgc catggcctat
     2101 cttcactggg tgcccgaact ggacaccggc atccccgaaa tcgacacgca gcacaagcgg
     2161 attctcgact acatcaacaa gctgcacgac ctgcgcgaca gccccgatcg cgctgcgctg
     2221 ggcgatgtga tcggcgagac ggtggactac acgctgtcgc actttgcttt cgaagaaagt
     2281 gtgatcgaaa gcgccggcta catgtttgcc ggcccgcaca agaaggtgca cgagctgttc
     2341 acccgcaagg tggccgagat gcagagccgc ttcgacgccg gcgaggacgt ggcggccgag
     2401 ctgcacggca tgctgtcgcg ctggctgttc aaccacatcc gcaacgaaga ccacggctac
     2461 atcgactcgg tcaaggccta tctgcgcatg acctccaagc agcgcgaggc cgacaaggcc
     2521 gaattgaagg ccgagctgct gcaggaactg gccgctcggc agaacggcaa gaagggctgg
     2581 ttcgcccggc tgttcggcgg ctgagccggc cctgccggcg ccgccgggcg gtcggtcagc
     2641 tggccgcttc cagaccgctc tggaaatgtg cctgcatgca cgcggcggcg gcggcgccgt
     2701 cgcgcgccgc aatggcctgc atcagcgccc ggtgttcggc cagcgaggcc tgcaggcgcc
     2761 cggccttctg cagtgaatgg cggcggtgca gcttcatcac cttgcgcaga tcctgcacca
     2821 tctgcagccg ccagcggttg tcggccagct ccagcaggcg cagatggaac gcttcgttga
     2881 gcgcaaaaaa gcgcgccgca tcccccaccg cggcttccag ttcggcgtgc agggcggcga
     2941 gttcggcacg gccggcggcg tcggcgtgca gcgccaccgc ctgagcggcg tcggtttcca
     3001 gcagcgccag caggtggtag acgtcggcca ggtccttgcc ggaggtctcg gtcacgtagg
     3061 cgccgcgccg caccttcatc gtcaccagcc cctcggccgc cagcaccttg agggcctcgc
     3121 gcagcggcgt gcggctgatg cccagctcgt cggcgatttt cagctcgtcg atccaatcgc
     3181 ccggcgccaa ctcgccgctg aagatgcgct ggcgcaggcg ctcggcaacc tcttcgtaga
     3241 gggcgcgggg ggcaaggcgg gcggcgctgt tcatggggcg ggccgggacg gggcgaaacg
     3301 ttgggccgcc cgcccgggca aaaaatctgc ggccattgta agccagacac tgcattttta
     3361 attaataatt atggaaagtc agccgccgcg cctgcggggt gttgcacctg ccttcatggc
     3421 catccggcaa cacagccgcc gcagcttgcg cacaatcgcc tgcgggcgcg tcggccacgg
     3481 cacgccccac cagaccgccc cgaaccggaa cccgccatga gccaagaccc cgcttccttc
     3541 acacccgcca ccctggccga ctggcgcaag gccgccgcca agtcggcacc gggcggcgac
     3601 ctgagcgccc tcgactggca cacgcccgac ggcatcacgg tcaagccgct gtacaccgcg
     3661 gccgacgtgc agggcctgcc gcatgccgac acgctgcccg gcttcgagcc cttcgtgcgc
     3721 ggcccgcagg ccaccatgta cgccgtgcgg ccctggacca tccggcagta cgcgggcttt
     3781 tccaccgccg aggaatccaa cgccttctat cgcaaggcgc tggccgccgg cgggcagggc
     3841 gtgtcggtgg cgttcgatct ggccacgcac cgcggctacg acagcgacca cccgcgcgtc
     3901 acgggcgatg tcggcaaggc cggcgtggcg atcgacagcg tggaggacat gaagatcctg
     3961 ttcgacggca tcccgctcga caaggtcagc gtgtcgatga ccatgaacgg cgccgtgctg
     4021 ccggtgctgg ccggctacat cgtcgccgcc gaggagcagg gcgtgcgcca ggaccagctg
     4081 gccgggacca tccagaacga catcctcaag gagttcatgg tccgcaacac ctacatctac
     4141 ccgccagggc cctcgatgcg catcatcggc gacatcatcg agtacacggc ccagcacatg
     4201 ccgaagttca actcgatctc gatcagcggc taccacatgc aggaggcggg cgccacgcag
     4261 gcgctggagc tggccttcac gctggccgac ggcaagcaat acgtcaggac cgcgctggcc
     4321 aagggcatgg acgtggatgc ctttgccggc cgcctgtcgt tcttctgggc catcgggatg
     4381 aacttctatc tcgaaatcgc caagatgcgc gccgcgcgcc tgctgtggtg ccgcatcatg
     4441 aagggcttcg gcgccaagaa tcccaagagc ctgatgctgc gcacccattg ccagaccagc
     4501 ggctggagcc tgaccgaaca ggacccctac aacaacgtgg tgcgcaccgc catcgaggcc
     4561 atggcggcgg tgttcggcgg cacgcagagt ctgcacacca acgcactcga cgaggcgatc
     4621 gcactgccga ccgagttttc ggcccgcatc gcgcgcaaca cccagctcat catccaggaa
     4681 gagacccaca tcaccagcgt ggtcgatccc tgggctggca gctacatgat ggagtcgctg
     4741 acccagcaga tggcggacgc cgcctgggcc atcatcgaag aggtcgacgc catgggcggc
     4801 atgaccaggg cggtggacag cggctgggcc aagctcaaga tcgaagctgc cgcggccgaa
     4861 aaacaggccc gcatcgactc cggcaaggac gtcatcgttg gcgtcaacaa atacaagctg
     4921 gccagggaag acgcggtcga catcctggaa gtggacaacg tcaaggtgcg tgaagcgcag
     4981 atcgcccgcc tcaaggatat caagcaaaaa cgcgacccag cgcaggtgca gcaagcgctg
     5041 gcagctatta caacgatagc acaaaacggg cagggcaacc tgctggcggc cgccgtcgat
     5101 gccattcgtg cccgcgccac cgtgggcgaa gtgagcgatg ccatggaaca ggtcttcggg
     5161 cgccaccgcg ccgacacgca gaaagtcacc ggcgtctacg ccgccgccta cgaaggcgct
     5221 gaaggctggg acaagctcaa gcaggagatc gccggcttcg ccgaggccga gggccgccgc
     5281 ccgcgcgtga tgatcgccaa gctgggccag gacggccacg accgcggcgc caaggtggtg
     5341 gccaccgcct ttgccgacct gggcttcgac gtcgacatcg gcagcctgtt ccagaccccc
     5401 gaggaatgcg cgcgccaggc gatcgagaac gacgtgcacg cggtcggcgt cagcacgctg
     5461 gcggccggcc acaagaccct ggtgccggcc atcatcaatg aattgaagaa gcaaggcgcg
     5521 gacgacatcg tggtcttcgt cggcggcgtg gtgccgcgcc aggactacga cttcctgtac
     5581 gacgccggcg tcaagggcat ctacggcccc ggcaccccca tcccggccag cgccaaggat
     5641 gtgctggagc agatccgcca ggcgcgtcca tgacggctcc caccgcttgg gcttcgaccg
     5701 cctcagcacg aacgagaggg cggatgtgag ccccgccgta tccgcccagg ccatcacgca
     5761 gagccgaggc ccggcccagc gccgcgccat tgccaaggcc atcacgctgc tcgaatcgac
     5821 ccgtgccgac caccgggcgc agggcgacga gctgctgacg gcgctgctgc cgcacaccgg
     5881 cggtgcgctg cgcctgggca tcagcggcgt gccgggggtg ggcaagagca ccttcatcga
     5941 agcgctcggg gtgtatctgg tggagcgcgg ccaccgggtg gcggtgctgg cgatcgaccc
     6001 ttcgtccacc gtctcgggcg gctcgattct gggcgacaag acgcgcatgg aacgcctgtc
     6061 ggcgctggaa ggcgcctaca tccgccccag ccccagcagc ggcacgctgg gcggcgtggc
     6121 cgagaaaacg cgcgaggcca tgctggtgtg cgaggcggcc ggctacgacg tggtgctggt
     6181 cgaaaccgtg ggcgtcggcc agagcgaaac cgccgtgcac ggcatgaccg acatgttctg
     6241 cgtgctgcag ctgcccaacg cgggcgacga cctgcaggcg atcaagaaag gcgtgatgga
     6301 gctggccgac ctggtggtca tcaacaaggc cgacctcgac ccggcggcgg ccacccgggc
     6361 gcgggcgcag atccagtcgg cgctgcgcgt gttcggccag cagggcaatc cgcagcatgc
     6421 gctgcaggac gccagcgtct ggcacccgga ggtgatccag ctcagcggcc tgaagggcga
     6481 gggcctggat gcgttctggg actgcgtgag ccggttccgc gccctgcaga gcgccggcgg
     6541 ccggctgcac gagcgacgtc ggcagcagtc gctggcctgg atgtgggagc gcatcgatgc
     6601 cggcctgaaa gcggccttcc gcgcccaccc cgaggtgcgc caggcgctgc cggccatgac
     6661 gcaggacgtc gcccagggcc ggctgccgcc gtccaccgcg gcgcgccggc tgctggcgct
     6721 ggtggcgccg gagagcgtcg cgtgaccgcc atggcgctca gcccgcagga cctgcaacgc
     6781 atcgcgcagt cgcccggctg gcgcggcggg cgggttgagg tctgcgacac ctcgcagggc
     6841 aaggtgatcg tcaagggctg gcgaccggcc catcacccgg cccgctatgg cctgctcaac
     6901 cgcctggcgc gctggctggg gctgccgttt ctgcgcgccg tgccgctggc cggcggcgcc
     6961 caggcgcagc acaccgaggt ggcccgcctg cgcgcgctgc gccgcgccgg cgcgcgcgtg
     7021 cccgaggtgc tgcacgtggg cagcgaccat ttcgtcatgc agtggctggg acgcgcccac
     7081 ctgggcgacg tgctgcagtc ggggcacccg ggcgcggccg cgctgtggcg cgaggccggc
     7141 gacgagctgg tgcgcctgca ccgggccggc cagtacctca gccagggctt tgcgcgcaac
     7201 atgatcgtca ccgacgaagc cggcgacacc ccgcgcctgg ccgggctgat cgacttcgag
     7261 gacgacccgc tgcaggtcat gggtctggcc gacgcccagg tgcgcgactg gctggccttc
     7321 ctgcacagca ccctgtggac cttgcccttg gcgccgtccg aggtggacgc ctgcatcgac
     7381 gcctggctgg acgccgaaag cccggccgtg cgcgccggct ttgcccaggc ctgccggcgc
     7441 ctggcctggc tgcgggtgct gccgcgccaa cgccgatttg gccgcgacac ggtggcgttg
     7501 caggccgccg ccgcagcggc gcaccgccac gcccgccgtc acccgatctc ccttgataac
     7561 cgcagcaact gaaggacccc catgaaaccg atcctggaac aactggagca aaagcgcgtc
     7621 gccgcccgcc tgggcggggg gcaaaagcgc atcgacgcgc agcacgccaa gggcaagctc
     7681 acggcgcgcg agcgcatcga ggtgctgctg gacgccggca gcttcgaaga gtgggacatg
     7741 ttcgtcgagc accgctgcgc cgacttcggc atggacgcca cccacatccc cggtgacggc
     7801 gtggtgaccg gctacggcac catcaacggc cgcctggtgt tcgtcttcag ccaggacttc
     7861 acggtgttcg gcggcgccct gagcgaggcg cacgccgaga agatctgcaa ggtgatggac
     7921 caggccatga aggtcggcgc gccggtgatt ggcctgaacg actcgggcgg cgcgcgcatt
     7981 caggagggcg tggccagcct gggcggctac gccgaggtgt ttcagcgcaa cgtgatggcc
     8041 agcggcgtga tcccgcagat cagcatgatc atggggccgt gcgcgggtgg cgcggtgtac
     8101 agcccggcca tgaccgactt catcttcatg gtcaaggaca ccagctacat gttcgtcacc
     8161 ggccccgagg tggtcaagac cgtgacgcac gagagcgtga cgtcggagca gctgggcggc
     8221 gcgctgaccc acaccacgcg cagcggcgtc gccgacctgg cgttcgagaa cgacgtcgag
     8281 gcgctgctga tgctgcggcg ctttttcaac tacgtgccgg ccagcaaccg cgaaaaggcg
     8341 ccgtaccggc cgacccagga cccggccgac cgcgccgacc tgagcctgga caccctggtg
     8401 ccggacaacc cgaacctgcc ctacgacatc aaggaagcca tcgtcaagac ggtggacgac
     8461 ggcgagttct tcgagctgca gcccgactac gccgccaaca tcgtgatcgg cttcggccgc
     8521 atggacggcc atgtggtggg catcgtcgcc aaccagccgc tggtgctggc cggctgcctg
     8581 gacatcaagt cgagcatcaa ggcggcgcgc ttcgtgcgct tttgcgatgc cttcaacatc
     8641 ccgatcctga ccttcgtcga cgtgcccggc ttcatgcccg gcaccagcca ggagtacggc
     8701 ggcatcatca agcacggcgc caagctgctg tacgcctacg ccgaatgcac cgtgcccaag
     8761 atcacggtga tcacgcgcaa ggcctacggc ggcgcctacg acgtgatgag ttccaagcac
     8821 ctgcgcggcg acgtcaacct ggcctggccc aacgccgaaa tcgccgtgat gggcgccaag
     8881 ggcgcggtgg aaatcatctt ccgcgaggaa aagaaggacc ccgccaagct cgccgcgcgc
     8941 gaggccgagt acaaggcgcg ctttgccaac cccttcgtgg ccggggcgcg cggcttcatc
     9001 gacgacgtga tcctgccgca cgagacgcgc cagcgcatct gccgcagcct ggccatgctg
     9061 aaagacaaga agctggacaa cccgtggcgc aagcacggca acattccgct ctgaaaatca
     9121 tagctgcccg cgcaatgccc aaggccgcca acgctcaact ggcccgtcag ttgacggaca
     9181 tcccgaacat cgggccgtcg atcgcgtccg acctgcgcct gctgggcatc cgctcgcccg
     9241 ccgatgtgcg cgccatggac ccgctggcgg cctacgatgc cctgcgtacc cccatggggc
     9301 accgccacga cccgtgcgtg ctggacacct tcctggccgc gcacgacttc atgcatggcg
     9361 gccaggcgcg cccatggtgg cacttcaccg ctcgacgcaa ggccttgctc gcctccctga
     9421 aacgctgacg agagataccg acatgttcaa ccagatcctg attgccaacc gcggcgaaaa
     9481 cggccgcgcg gcggacgttt cgaccaatgg cttgccgcag cccgcgcagc gggcttctgc
     9541 acgccacacc accgcttgag gaaccgcaac catgtttacc aaaatcctga tcgccaaccg
     9601 gggcgagatt gcgtgcaggg tcatcgccac cgcccgcaag atgggcatcg ccaccgtggc
     9661 ggtgtattcc gacgccgacg cgcaggcccg cttcgtgcag atggccgacg aggccgtgcg
     9721 tctggggccg ccgccctcgc gcgagtccta cctgctgggc gacaagatca tcgaggcggc
     9781 caaggccacc ggggcgcagg cca
//
LOCUS       DAOOOG010000095         9654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_8206_length_9654_cov_22.637358, whole genome shotgun sequence.
ACCESSION   DAOOOG010000095 DAOOOG010000000
VERSION     DAOOOG010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9654
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8206_length_9654_cov_22.637358"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..736)
                     /locus_tag="PK242_08235"
     CDS             complement(71..736)
                     /locus_tag="PK242_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ94045.1"
                     /translation="MKKLNKVAALFAVATVAGSAFAAGSTINSDNWRNGHGNLIWKNG
                     TNELCWRDAGWTPATAAPGCDGALEAPKAAAPAAAPAPAAAAPAPAPAKAPAPAPAVA
                     AQKVTYAADAFFDFDKSVLKPEGRAKLDDLVSKIQGINLEVIIAVGHTDSIGSDAYNQ
                     RLSVRRAEAVKAYLVSKGIERNRVYTEGKGEKQPVADNRTAQGRAKNRRVEVEVVGTR
                     AAK"
     gene            990..3647
                     /gene="gyrA"
                     /locus_tag="PK242_08240"
     CDS             990..3647
                     /gene="gyrA"
                     /locus_tag="PK242_08240"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOZ94046.1"
                     /translation="MTSFAKETLPISLEEEMRRSYLDYAMSVIVGRALPDARDGLKPV
                     HRRVLYAMHELNNDWNRPYKKSARIVGDVIGKYHPHGDSAVYDTIVRMAQDFSLRHML
                     VDGQGNFGSVDGDNAAAMRYTEIRLSKIAHEMLADIDKETVDFGPNYDGSEQEPLVLP
                     SRLPNLLVNGSGGIAVGMATNIPPHNLNEVVDACLHLLRQPTASIEELMEIMPAPDFP
                     TGGIIYGMQGVREGYRTGRGRVVMRAKVHFEDIDRGQRQAIIVDELPYQVNKKTLQER
                     MAELVHEKKLEGISHIQDESDKSGMRLVIELKRGEVPEVVLNNLYKQTQLQDTFGMNM
                     VALIDGQPRLCNLKELVEVFLQHRREVVTRRTVFNLRKARERGHVLEGLAVALANIDE
                     FIRIIRESPTPPVAKAELMTRSWDSQLVREMLTRTRADGGVVNADDYRPEGLERACGM
                     QGDGLYRLSDTQAQEILQMRLQRLTGLEQDKIIAEYKEVMAQIDDLLDILARPERVST
                     IIGEELSALKAEFGQTKVGARRSTVEHNAQDLATEDLITPTDMVVTLSHTGYIKAQPV
                     SEYRAQKRGGRGKQATATKEDDWIDQLFIANTHDWILCFSNRGRLYWLKVWEVPSGSR
                     GSRGRPIVNMFPLAEGEKINVVLALTGAMRNFPDDHFVFMATSMGTVKKTALPEFSNP
                     RKAGIIAVDLDEGDYLIGAALTDGAHDVMLFSDGGKAVRFDENDVRPMGRNARGVRGM
                     SLEEGQRVIAMLVADGETTTDSAEPNARNSVLTATENGYGKRTNINEYTRHGRGTKGM
                     IAIQQSERNGKVVAATLVSSDDEIMLITDKGVLVRTRVAEIREMGRATQGVTLIALDD
                     GAQLSGLQRIVENDAQDPAIDPTQESPTE"
     gene            3725..4240
                     /locus_tag="PK242_08245"
     CDS             3725..4240
                     /locus_tag="PK242_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HOZ94047.1"
                     /translation="MAQNDAKRALANKLVQLQLKTDGPAITDQLTNSAVQPIVAGWSQ
                     RLDETVPPARQKEVRDKLDVELKKFADSTHKSIEAQVGKAAEAAMVPIFMEKLSEEEM
                     KTIIAYMESPASAKLQALGADATDAWAKRIIDSTRSQVEASAKTFESAADRIVKAAAG
                     GASGAAAATKK"
     gene            4321..5430
                     /gene="pheA"
                     /locus_tag="PK242_08250"
     CDS             4321..5430
                     /gene="pheA"
                     /locus_tag="PK242_08250"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOZ94048.1"
                     /translation="MSSTTPVQSEALAQLRTQIDSLDAQLLTLLNQRAKVAEQVGELK
                     RHEGTPFFRPDRVAQVIDKIQASNTGPLQNSHVAAIWREIMSACLALEVPQRVAVLGP
                     QGTFCEQAAIEYFGSAANLIYCSSFDEVFHATAAGTAQFGVVGMENTTEGVVARSFDL
                     FLHSPVHVIGEVSLLVRHNLLRQVNALDKVEVVLAHPQALMQCQGWLSEHLPGAERRA
                     VSSNAEGARLAATNPAWAALASERAAAQFGLHIVAHAIQDEAYNRTRFAIICLPQTLG
                     TPAATGRDCTSIVVSVPNKPGSVHDMLTPLKKHGVSMTRFESRPAKSGKWEYFFYIDL
                     QGHPGEPNVAAALSELQAYCAFYKMLGAYPLGQDV"
     gene            5423..6295
                     /locus_tag="PK242_08255"
     CDS             5423..6295
                     /locus_tag="PK242_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOZ94049.1"
                     /translation="MFEQLGLIGCGLMGGSFALALKRAGLVKRVVGYSKSPSTTARAL
                     QLGVIDVEAPSALLAVSGADLVLLAVPVAATETTLKAIRHLVNGDMLIMDVGSSKRDV
                     VDAARRVLKQDVGVFVPCHPIAGREVSGVEHADAGLYAGKQVILTPIERTLTAKLEQA
                     NALWQALGCQVVNMSPESHDAAFAAVSHLPHLVAFALMNSIVNQPEGREYLSLAGPGF
                     RDFSRIAASDPQMWRDVLIANREELGRQTELFQHALNDLQRLIANGQADDLERLINRA
                     SHARANWRLSSGAR"
     gene            6312..8318
                     /locus_tag="PK242_08260"
     CDS             6312..8318
                     /locus_tag="PK242_08260"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HOZ94050.1"
                     /translation="MFDLPFLDIPALRAVHGTVRLPGSKSISNRVLLLAALCEGRTVI
                     HDLLDSDDTRVMLQALRQLGCVVELQGTSHVIEGLGNRPCQPQAELFLGNAGTAMRPL
                     TAALAVLGGDYRLSGIPRMHERPIGDLVDALRTLGCRIDYLGQAGYPPLRIGAPDLSL
                     DRPIPVRGDVSSQFLTALLLALPLAAGRPGARDIVIDVVGELISKPYIDITLQLLARF
                     GVTVQRQGWQRFVIPSGSRYRSPGEIHVEADASSASYFIAAGALSEGAAGQNGLTIEG
                     VGEASIQGDIRFVEAARQMGAQIESGPNWLRVRRGAWPLRAIDLDGNHIPDAAMTLAV
                     MALYADGPSTLRNIASWRVKETDRIAAMANELRKLGAQVEEGADFIRIHPPAGAAAWR
                     AASIHTYDDHRMAMCGSLAAFNPAGLPVRIEDPRCVGKTFPDYFETLFALAGAAGPVP
                     VICIDGPTASGKGTLAAELARRLGFRFLDSGALYRLVGLAARQQGMAPDATNEAALAS
                     LARQLPVRFEAQTVWLADQDVTDAIRTEAAGMDASRVSALPAVRQALLALQRDFARLP
                     GLVADGRDMGTVVFPDARLKVFLTADAAQRAERRYKQLISKGISANIDSLRADLEARD
                     ARDRDRAAAPLKAAPDALLLDNSLLSIAQSVEQVLSWWQARQPF"
     gene            8541..>9654
                     /locus_tag="PK242_08265"
     CDS             8541..>9654
                     /locus_tag="PK242_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOZ94051.1"
                     /translation="MSESFAQLFEESLQRTEMRPGEVITAEVVRVEHNFVVVNAGLKS
                     EAYIPIEEFKNDQGEVEVEPGAFVPVAIGSIENGYGDTILSRDTAKRLASWLALEKAL
                     ESGEFVTGTTAGKVKGGLTVLVNGIRAFLPGSLVDTRPTKDLTPYENKTLEFKVIKLD
                     RKRNNVVLSRRAVVEASMGEERAKLMETLKEGAIVNGVVKNITEYGAFVDLGGIDGLL
                     HITDMAWRRVRHPSEVVQAGQEITAKILKFDTEKNRVSLGLKQMGDDPWLGVSRRYPQ
                     GTRMFGKVTNIADYGAFVELEPGIEGLVHVSEMDWTNKNVAPSKIVSMGDEVEVMVLE
                     IDEDKRRISLGMKQCRANPWQEFAQNTKRGDRVKGPI"
BASE COUNT         1783 a         3264 c         3118 g         1489 t
ORIGIN      
        1 gtcaaacaaa aaggccctgg gagctttcgc ccccaaggcc tgatgcaatc cagtgatgaa
       61 gctggaccga ttacttcgcg gcgcgcgtgc cgaccacttc cacctcgacg cggcggttct
      121 tggcgcggcc ctgggcggtg cggttgtcgg ccaccggctg cttctcaccc ttgccttcgg
      181 tgtagacgcg gttgcgttcg atgcccttgg acaccagata ggctttcacg gcctcggcgc
      241 gacgcaccga cagtcgctgg ttgtaggcgt cggaaccgat cgagtcggtg tggcccacgg
      301 caatgatcac ttccaggttg atgccctgga tcttgctgac caggtcatcc agcttggcac
      361 ggccttccgg cttgagcacc gacttgtcga agtcgaagaa ggcgtcggcg gcgtaggtga
      421 ccttctgggc agccacggca ggcgccggag caggtgcctt ggccggagca ggtgccggag
      481 cggcagcagc cggtgccggt gccgcggccg gcgcagcagc cttgggcgcc tcgagcgcgc
      541 cgtcgcagcc aggagcagcg gtagccggcg tccagcccgc atcgcgccag cacagttcgt
      601 tggtgccgtt cttccagatc aggttgccgt ggccattgcg ccagttgtcg gaattgatcg
      661 tcgaaccagc tgcgaaggcg gaaccagcca cggttgccac tgcgaacaga gccgctactt
      721 tgttgagttt cttcatggtt ctcctcatgg gaaaaaagcc gcagccgggc tgcgataaat
      781 cggacgcttc aagtgaccac caccgaaaac gttgaggcga ttgtgccaca ggcgatagcg
      841 cgatcttggt ggaacggcgc ctcagcgcct cagacagagg ctcaatttcg atcaagtgtt
      901 gctatggaac cacaagtccg ggcgcctagt agaatcgatc gctttcgccc gccggatccc
      961 gccccgttgc tctccttgtt gctcggtcta tgacttcatt cgccaaagaa accctcccca
     1021 tcagtctgga agaggagatg cggcgaagct atctcgatta cgccatgagc gtgatcgtgg
     1081 gccgcgccct gcccgacgcg cgcgatggcc tgaagcccgt gcaccggcgc gtgctgtacg
     1141 ccatgcacga gctgaacaac gactggaacc ggccgtacaa gaagtcggcg cgcatcgtgg
     1201 gtgatgtgat cggcaagtac cacccgcacg gcgacagcgc ggtgtacgac accatcgtgc
     1261 gcatggcaca ggacttctcg ctgcgccaca tgctggtcga tggccagggc aacttcggca
     1321 gcgtggatgg cgacaacgcc gccgccatgc gttacaccga aatccgcctg tcgaagatcg
     1381 cccacgagat gctggccgac atcgacaagg agacggtgga cttcggcccc aactacgacg
     1441 gcagcgagca ggagccactg gtgctgccca gccgcctgcc gaatctgctg gtcaacggct
     1501 cgggcggcat tgccgtgggc atggccacca acatcccgcc gcacaacctg aatgaagtgg
     1561 tggacgcctg tctgcacctg ttgcgtcagc cgacggccag catcgaggaa ttgatggaga
     1621 tcatgccggc accggacttt ccgaccggcg gcatcatcta cggcatgcag ggcgtgcgcg
     1681 agggctaccg caccgggcgt gggcgcgtgg tgatgcgcgc caaggtgcac ttcgaggaca
     1741 tcgaccgcgg ccagcgccag gccatcatcg tcgacgagct gccctaccag gtcaacaaga
     1801 agacgctgca ggagcgcatg gccgagctgg tgcacgagaa aaagctcgaa ggcatcagcc
     1861 acatccagga cgagtccgac aaatccggca tgcgcctggt gatcgagctc aagcgcggcg
     1921 aagtgcccga ggtggtactc aacaacctgt acaagcagac ccagctgcag gacaccttcg
     1981 gcatgaacat ggtggcgctg atcgatggtc agccccggct gtgcaatctg aaggagctgg
     2041 tcgaggtctt cctgcagcac cgccgcgagg tagtgacccg gcgcaccgtg ttcaacctgc
     2101 gcaaggcgcg cgagcgcggc catgtgctgg aaggcctggc cgtggcgctg gccaacatcg
     2161 acgagttcat ccgcatcatc cgcgaatcgc ccacgccgcc cgtggccaag gccgagctga
     2221 tgacgcgcag ctgggacagc cagctcgtgc gcgagatgct gacgcgcacg cgcgcagacg
     2281 gcggcgtggt caacgccgac gactaccgcc ccgagggtct ggagcgcgcc tgcggcatgc
     2341 agggcgatgg cctgtaccgc ctgtccgaca ctcaggcgca ggaaatcctg cagatgcgcc
     2401 tgcagcgcct gaccggcctg gagcaggaca agatcattgc cgaatacaag gaagtgatgg
     2461 cccagatcga cgatctgctg gacatcctgg cgcgccccga gcgcgtgtcc accatcattg
     2521 gcgaggagct gtcggcactg aaggccgaat ttggccagac caaggtcggc gcgcgccgtt
     2581 cgacggtcga acacaacgcc caggatctgg ccaccgagga cctgatcacg cccaccgaca
     2641 tggtggtcac gctcagccac accggctaca tcaaggcgca gccggtatcg gaataccggg
     2701 cccagaagcg tggcggtcgc ggcaagcagg ccacggcgac caaggaagac gactggatcg
     2761 accagctgtt catcgccaac acgcacgact ggatcctgtg cttttccaac cgcggccgcc
     2821 tgtactggct caaggtctgg gaagtgccgt ccggttcgcg cggctcgcgc gggcgcccca
     2881 tcgtcaacat gttcccgctg gccgagggcg agaagatcaa cgtggtgctg gcgctcaccg
     2941 gcgcaatgcg caatttcccg gacgatcact tcgtgttcat ggccacctcc atgggcacgg
     3001 tcaagaaaac cgccctgccc gagttcagca acccacgcaa ggccggcatc atcgccgtcg
     3061 acctggacga gggcgactac ctgatcggcg ccgccctgac cgatggtgcg cacgacgtga
     3121 tgctgttctc cgacggcggc aaggcggtgc gcttcgacga aaacgacgtg cgcccgatgg
     3181 gccgcaacgc gcgcggcgtg cgcggcatgt cgctggaaga aggccagcgc gtcatcgcca
     3241 tgctggtggc cgacggtgaa accaccaccg acagcgcgga accgaatgcg cgcaacagtg
     3301 tcttaaccgc aaccgaaaac ggttacggca agcgcaccaa tatcaacgaa tacacacgcc
     3361 acggtcgtgg caccaagggc atgatcgcga tccagcagag cgagcgcaac ggcaaggtgg
     3421 ttgccgccac gctcgtgagc agcgacgacg agatcatgct catcaccgac aagggcgtgc
     3481 tggtgcgcac ccgtgtcgcc gaaatccgcg aaatgggccg cgccacccaa ggtgtcacgc
     3541 tcatcgcgct ggacgacggc gcccagttga gcggtctgca gcgcatcgtc gaaaacgacg
     3601 cgcaggatcc cgccatcgac cccacccagg aatccccaac ggagtgaacc cagtgaattt
     3661 gaccatttcc cgcctcccga cagctattgt tttcgtagct gcttgcgcac tatccacggg
     3721 cgccatggcc caaaatgatg cgaaacgtgc gctggccaac aagctggtgc agctgcagct
     3781 caagaccgat ggtccggcca tcaccgatca gctcaccaac agtgcggtgc agcccatcgt
     3841 ggccggctgg tcgcagcgac tcgatgagac cgtgccgccg gcccgccaga aggaggtgcg
     3901 cgacaaactc gacgtcgagc tgaagaagtt cgccgacagc acccacaagt cgatcgaagc
     3961 gcaggtgggc aaagcggccg aagcggcgat ggtgccgatc ttcatggaga aactctccga
     4021 agaggagatg aagaccatca tcgcctacat ggaatcgccc gccagcgcca agcttcaggc
     4081 gctgggagca gacgccaccg acgcctgggc caagcgcatc atcgacagca cgcgcagcca
     4141 ggtggaagcc agcgccaaga ccttcgagag cgcggccgat cggatcgtga aagcggcggc
     4201 gggcggcgca tccggcgccg ctgcggccac caagaaataa ggtcccgacc ccagccaccc
     4261 acgtgccggc aggcgcccgt ccgaggcgct gcggcccgcc ccaggccatc ccgtcaccgc
     4321 atgagctcaa ctacgcccgt ccagtccgaa gccctggccc agctgcgcac gcagatcgac
     4381 agcctggatg cgcagttgct gacgctgctc aaccagcgcg ccaaagtggc cgagcaggtg
     4441 ggcgagctca agcgccacga aggcacgccg ttctttcgcc ccgaccgcgt ggcgcaggtg
     4501 atcgacaaga tccaggccag caacaccggg ccgctgcaga acagccatgt ggcggcgatc
     4561 tggcgcgaaa tcatgtcggc ctgcctggcg ctggaagtgc cgcagcgcgt ggctgtcctg
     4621 gggccgcagg ggacgttctg cgagcaggcg gcgatcgagt acttcggcag tgccgccaac
     4681 ctgatctact gcagcagctt cgacgaggtg tttcacgcca ccgcggccgg cacggcccag
     4741 tttggcgtgg taggcatgga aaacaccacc gagggcgtgg tcgcgcgctc gttcgacctg
     4801 tttctgcatt cgccggtcca tgtgatcggc gaagtcagcc tgctggtgcg gcacaacctg
     4861 ctgcggcagg tcaacgcgct ggacaaggtc gaggtggtgc tggcgcaccc gcaggcgctg
     4921 atgcaatgcc agggctggct cagcgagcac ctgccgggcg ccgagcgccg ggccgtctcc
     4981 agcaacgccg aaggtgcgcg cctggcggcc accaacccgg cctgggcggc tctggccagt
     5041 gagcgtgccg ccgctcaatt cgggctgcac atcgtcgccc atgcgatcca ggacgaggcc
     5101 tacaaccgca cgcgctttgc catcatctgc ctgccgcaga cgctgggcac gccggcggcc
     5161 acgggtcgcg actgcaccag catcgtggtg tcggtgccca acaaaccggg ctcggtgcac
     5221 gacatgctga caccgctgaa gaagcatggg gtgagcatga cgcgcttcga gtcgcgcccg
     5281 gccaaatcgg gcaagtggga atacttcttc tacatcgacc tgcaaggtca tcccggcgag
     5341 cccaatgtgg cagcggcgct gagcgaactg caggcctact gcgccttcta caagatgctg
     5401 ggcgcctatc ccctgggtca agatgtttga gcaactcgga ctgatcggct gcggcctgat
     5461 gggcggctcc ttcgccctgg cgctcaaacg cgccggcctg gtcaagcgcg tggtgggtta
     5521 cagcaaatcg ccctccacca cggcgcgggc gctgcaactg ggcgtgatcg acgtcgaggc
     5581 accctcggcc ctgctggcgg tgtccggcgc cgacctggtg ctgctggcgg tgccggtggc
     5641 cgccaccgaa accacgctca aggccatccg ccacctggtc aacggcgaca tgctgatcat
     5701 ggacgtcggc tccagcaagc gcgacgtggt ggacgccgcg cggcgcgtgc tgaagcagga
     5761 cgtgggcgtg ttcgtgccct gccacccgat cgccgggcgc gaggtgtcgg gggtggagca
     5821 cgccgacgcc gggctgtacg ctggcaagca ggtcattctg acccccatcg agcgcacgct
     5881 gacggccaag ctggagcagg ccaacgccct gtggcaagcg ctcggttgtc aggtggtcaa
     5941 catgtcgccc gaatcgcacg acgccgcctt cgcggcagtc agtcacttgc cgcatctggt
     6001 cgccttcgcg ctcatgaaca gcatcgtgaa ccaacccgaa gggcgcgaat acctgtcgct
     6061 ggccggcccg gggtttcgcg acttcagccg catcgccgcc agcgacccgc aaatgtggcg
     6121 cgacgtgctg atcgccaatc gcgaagagct ggggcgccag accgaactgt ttcagcatgc
     6181 gctcaacgac ctgcagcgac tgatcgccaa cggccaggcc gacgatctgg aacgcctgat
     6241 caaccgcgcc agccatgcgc gcgccaactg gcgcctgtcg tcaggtgccc gctgagcgcg
     6301 ccggccgcac cgtgttcgat cttccgtttc tcgacatccc ggccctgcgc gcggtgcacg
     6361 gcacggtgcg tctgccgggc tccaaaagca tctccaaccg cgtgttgctg ctggccgctc
     6421 tgtgcgaagg gcggaccgtc attcacgacc tgctggactc cgacgacacc cgcgtgatgc
     6481 tgcaggcgct gcgccagctc ggctgcgtgg ttgaactgca gggcacctcg cacgtcatcg
     6541 aaggcctggg caaccggccg tgccagccgc aggccgaact gttcctgggc aatgccggca
     6601 ccgccatgcg gccactcacc gcagcgctgg ccgtgctggg cggcgactac cgactcagcg
     6661 gcatcccgcg catgcacgag cgcccgatcg gcgacctggt cgatgcgctg cgcacgctcg
     6721 gctgccgcat cgactacctt ggccaggccg gctacccgcc gctgcgcatc ggcgcaccgg
     6781 acctcagcct ggatcggccc atccccgtgc gcggcgacgt ctccagccag ttcctcaccg
     6841 cgctgctgct ggcactgccg ctggcggccg gccgccccgg cgcgcgcgac atcgtcatcg
     6901 acgtggtcgg cgagctgatc tccaagccct acatcgacat cacgctgcag ctgctggcgc
     6961 gctttggcgt caccgtgcag cgccagggct ggcagcgctt cgtcatcccc agcggcagcc
     7021 gctaccgatc accgggcgaa atccacgtcg aggccgatgc ctcgtctgcc agttatttca
     7081 tagctgcggg cgctttgtca gagggcgctg caggccagaa tggcttgacg atcgaagggg
     7141 ttggcgaagc gtccatccag ggcgacatcc gcttcgtcga ggccgcgcgc cagatgggcg
     7201 cgcagatcga aagcggcccc aactggctgc gcgtgcggcg cggcgcctgg ccgctgcgcg
     7261 ccatcgatct ggacggcaac cacattcccg acgccgccat gacgctggcg gtgatggcgc
     7321 tgtacgccga cggccccagc accttgcgca acatcgccag ctggcgcgtc aaagaaaccg
     7381 accgcatcgc cgccatggcc aacgagctgc gcaagctcgg cgcccaggtg gaagaaggcg
     7441 ccgacttcat ccgcatccac ccacctgccg gtgccgcggc gtggcgggcg gcgtcgatcc
     7501 acacctacga cgaccaccgc atggccatgt gtgggtcgct ggcggcgttc aaccccgctg
     7561 ggctgccggt gcgcatcgaa gacccgcgtt gcgtcggcaa gacctttccc gactacttcg
     7621 agacgctgtt cgccctcgca ggtgcggccg gcccggtgcc ggtgatctgc atcgacggac
     7681 ccacggcctc cggcaaaggc acgctggcgg ccgaactggc ccgccggctg gggttccggt
     7741 ttctggattc cggcgcactg taccggctgg tcggtctggc cgcgcgccaa cagggcatgg
     7801 caccggacgc caccaacgag gccgcccttg ccagcctcgc gcgccagttg ccggtgcgtt
     7861 tcgaggccca gacggtctgg ctggccgacc aggacgtcac cgacgccatt cgcacggaag
     7921 cggcgggcat ggacgcctcg cgcgtttcgg ccctgcccgc ggtgcgccag gcgctgctgg
     7981 ccctgcagcg cgactttgcc cggttgccgg gtctggtggc cgatgggcgc gacatgggaa
     8041 cggtggtgtt tccagacgcc cggctcaagg tgtttctgac cgcagacgcc gcccagcgcg
     8101 ccgaaaggcg gtataagcag ctgatttcca agggaatttc tgctaacatt gatagtcttc
     8161 gcgccgacct ggaggcgcgc gacgcgcggg atcgagaccg cgccgccgcg ccactgaagg
     8221 ccgcgccaga cgccctgctg ctggacaact ccctgctttc gattgcgcag tcggtcgaac
     8281 aggtgctgag ctggtggcag gcacggcagc cgttctgatc gacatctcga cctagaacgc
     8341 agccacagcg ccagaacccc cggttctggc gttcttcggt ccacccggcc tccctgccgc
     8401 gcttgctcag cgcaccaggc ctggtggttg ttcaacccgc aacccgcggc tctggccgcc
     8461 acccccgccg ccgcacagcc tctggaaacg attgcatggt gccgtcgtga gaacccgtgc
     8521 gcgcgcaaca aggaaacccc atgtctgaat cttttgccca gttgtttgaa gaatccctgc
     8581 agcgcaccga gatgcgcccg ggtgaagtca tcaccgccga agtggtgcgc gtcgagcaca
     8641 acttcgtcgt cgtgaacgcc ggcctgaagt ccgaggccta catcccgatc gaggaattca
     8701 agaacgacca gggcgaggtc gaggtggagc ccggcgcctt cgtgccggtg gccatcggtt
     8761 cgatcgaaaa cggctacggc gacaccatcc tgagccgcga caccgccaag cgcctggcct
     8821 cgtggctggc gctggaaaaa gccctggaat cgggcgagtt cgtcaccggc accaccgccg
     8881 gcaaggtcaa gggcggcctg acggtgctgg tcaacggcat ccgcgccttc ctgcccggct
     8941 cgctggtcga cacccgtccg accaaggacc tgacgcccta cgagaacaag acgctcgaat
     9001 tcaaggtcat caagctcgat cgcaagcgca acaacgtggt gctgagccgc cgcgccgtgg
     9061 tcgaggccag catgggcgaa gagcgcgcca agctgatgga gacgctgaag gaaggcgcca
     9121 tcgtcaacgg cgtggtcaag aacatcaccg aatacggtgc cttcgtcgac ctgggcggca
     9181 tcgacggcct gctgcacatc accgacatgg cctggcgccg cgtgcgccac ccctccgagg
     9241 tggtgcaggc tggtcaggaa atcactgcca agatcctcaa gttcgacacc gaaaagaacc
     9301 gcgtcagcct gggcctcaag caaatgggcg acgacccgtg gctgggcgtc agccgccgct
     9361 acccgcaggg cacgcgcatg ttcggcaagg tcaccaacat cgccgactac ggcgccttcg
     9421 tcgaactgga gccgggcatc gaaggtctgg tgcacgtgtc tgaaatggac tggaccaaca
     9481 agaacgtcgc gcccagcaag atcgtgtcga tgggcgacga ggtcgaggtc atggtgctgg
     9541 agatcgacga ggacaagcgc cgcatcagcc tgggcatgaa gcagtgccgc gccaacccct
     9601 ggcaggaatt cgcgcagaac accaagcgcg gcgaccgcgt caagggcccg atca
//
LOCUS       DAOOOG010000096         9617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_8245_length_9617_cov_25.148086, whole genome shotgun sequence.
ACCESSION   DAOOOG010000096 DAOOOG010000000
VERSION     DAOOOG010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8245_length_9617_cov_25.148086"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PK242_08270"
     CDS             <1..770
                     /locus_tag="PK242_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ94052.1"
                     /translation="FFAPPTVWIALLRSPLFDQTDLSKLAKGYYGASIMPVEVLKEIA
                     RRLPAVRLWNLYGQTEIAPLATMLGPDDQLRKPGSAGRAVLNVETRVVDDALRDVAVG
                     EVGEIVHRSPHLMLGYFHDDERTAAAFEGEWFHSGDLGTIDAEGYITVVDRKKDMIKS
                     GGENVASREVEEMIYRLPEVSEVAVIGLPDPKWVEAVTAVVVVKAGQELTEAQVIAHC
                     NAHMATFKAPKRVIFTDQLPKNPSGKLLKRQLRQTFV"
     gene            complement(911..1882)
                     /locus_tag="PK242_08275"
     CDS             complement(911..1882)
                     /locus_tag="PK242_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HOZ94053.1"
                     /translation="MATSLLALLDDIATVLDDVAVMSKIAARKSAAMADDVAVLTKVS
                     AQKTAGVLGDDLALNAQQVTGVSAHREIPVVWAVARGSFVNKAILVPVALLVSAFAPW
                     AVTPLLMLGGAFLCFEGCEKLLHKLLHTRAEDQAHEAELEAALADPQVDLAALEKDKI
                     KGAVRTDFILSAEIIAITLGAVAQADFWTQVAVLVGIAILMTVGVYGLVGGIVKLDDL
                     GLWLSRRAGAGAQALGRGILALAPWLMKGLSVAGTAAMFLVGGGIIVHGVPALHHLLE
                     GWAAGLGGVLRVLADMGFNAAVGMLVGAVVVAAVTLFQRLRGTPRTA"
     gene            2211..2690
                     /locus_tag="PK242_08280"
     CDS             2211..2690
                     /locus_tag="PK242_08280"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOZ94054.1"
                     /translation="MPLLLTRIAGRALACVGLMCAAAAMAQAPEAPAGDARQGTFKTV
                     RGDVTVVRDNTRVAAIVGAGVRSTDRILSGAESAAAVTLLDGTVLAIGPDSALDLSQY
                     QFNPTTQEGSVLVNLMRGSLRMVTGLIAKLKPEQVKVTTPTSVIGVRGTDFIVEENP"
     gene            2687..3271
                     /locus_tag="PK242_08285"
     CDS             2687..3271
                     /locus_tag="PK242_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ94055.1"
                     /translation="MRLAPLLTAVALLAAGCAPTTRVTLLPQADHPQAAVEVTARQRS
                     TRLAQPYASAAVRQQGPELEQLDAATVHKRYPALLAVMPPPAERFELKFITGGTRLTP
                     ESTAELSDVLASAIARAGGEIVVIGHTDRVGTLEANDALSLRRAEAVRALIVERGFEA
                     ARVRAVGRGEREPQVPTEDDVDEPRNRRVEIVVR"
     gene            3386..4030
                     /locus_tag="PK242_08290"
     CDS             3386..4030
                     /locus_tag="PK242_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ94056.1"
                     /translation="MAQRTQACTACHGEQGRAGPDGYYPRLAGKPAGYLYNQLLNFRD
                     GRRHYGLMTRMVDVLSDAYLMDIARHFAALQVPYPPPAPPASPPPPAVLERGRALALQ
                     GDAAQGVPACVQCHGRTLTGALPDVPGLLGLPADYLSAQLGGWRTGQRRAHAPDCMAR
                     IVQRLSDRDAHAVVSWLASQPVPADPRPAPARPPLPAEWADLRCGRAPEPEVAR"
     gene            4027..5307
                     /locus_tag="PK242_08295"
     CDS             4027..5307
                     /locus_tag="PK242_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOZ94057.1"
                     /translation="MSALRRLLLGLAGLALLGAGLLWQINFNDGVDTRSSAPVASDAA
                     TRARGAYLARAGNCLACHTARGGPAAAGGRPIATPFGTVYSSNLTPDARAGIGGWNAA
                     TFWRALHHGRSADGRLLNPVFPYTHTTGLTRADSDALFSYFLGLPPSATAVPAHELRW
                     PFGTQAALALWRALYFRPGSFQPDPARSEQLNRGAYLVQAVAHCSACHTPRDRLGGAD
                     WQSLSGGTVPGTGWYAPSLTARDEGAVGHWPAEQIVQLLATGAAPQGVASGPMAEVVY
                     QGTQHLSGADLQAVAAYLQHLPPQPGRQDTPAPRQRPSAAGERLYGQHCAACHGEDGQ
                     GVPGAYPPLSGNRSVTMANTQNLLQIVLFGGFGPATAGRPQPFGMPPFALTLSDAEIA
                     AVLSYIRGAWGNDAGEVAPLDVVRMRHVNHSGLP"
     gene            5453..6607
                     /locus_tag="PK242_08300"
     CDS             5453..6607
                     /locus_tag="PK242_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94058.1"
                     /translation="MALNWLMSKRARPQTEGDGAQRPDGRAAEAEANADDQELRWPEV
                     VRHFGQEVAMALNTAADQLERLSRLEPSLVGSLQTVTDAVERARQAGMAAQQVLRLTE
                     DPPVQHRELLGLDDVVRAVLTTRAEWLQRHQVGVRHGLAQTQVYADSSMLYMLVDELL
                     LWAAQMAPQIAIMVDRSSRSGRPRLSVLAWCDPAQVPEAAWRGMRWMLWHQLARSLGA
                     SARVDTQTDHVRVTLSLSAVTEEQLATGIEESVGPSSVSAVIQGCRVLIVSGNAQRRA
                     QSLQALTGYGLILDSAGDMTEAERLATRHLPDAVVYDGSVNPSAVAGLRESLEARAGT
                     PAAFIEIHDDDSASDFRTSTVGSVSTGHIAAGSLNQALGPALVFELCKVM"
     gene            6700..7389
                     /locus_tag="PK242_08305"
     CDS             6700..7389
                     /locus_tag="PK242_08305"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOZ94059.1"
                     /translation="MSAQPRHPASAAATTADPPLQVRYEDAHLIALDKPAGLLTVPGR
                     GTDKSDCLSRRAQARWPDALVVHRLDQATSGLLLLARGPAMQRALSQAFAQRQVHKRY
                     EALVHGRLTLPADATDGWARIDLPLIVDWPQRPRSKVDHVAGKPSQTRWRPLAHDAQT
                     RRTRLALEPLTGRSHQLRVHLLAIGHPIVGDTLYGPPDGAPRLMLHACALRLTHPVTG
                     QPLALDSAAPF"
     gene            7464..8054
                     /locus_tag="PK242_08310"
     CDS             7464..8054
                     /locus_tag="PK242_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HOZ94060.1"
                     /translation="MTRRNLQGLGLALSLALGGPWLSGCTITSTPQSQAAPAATRAAI
                     DAAVNETLSRLYHLVPGTQDMVSRAAGVLVFPSVLGGSFVVGAEHGKGALRVGGQTTS
                     YYSTTGASIGFQAGGQSRSVIYVFNTREALQSFRASNGWTADADATVAIGNVGANGRV
                     DSHTVQQPIASFVLTNVGLEAGVSVGGSRIVPIALP"
     gene            8118..8897
                     /locus_tag="PK242_08315"
     CDS             8118..8897
                     /locus_tag="PK242_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ94061.1"
                     /translation="MNRKPTRQLYVLTGASRGMGRAMAQQLLAPGHQLLTLSRRPDEA
                     LASQAGAAGATLTQWAVDLGDAAPVAARLADWLRAQDGKALTSATLINNAGAIPAIVP
                     LRDADPPALAAALRLGLEAPMQLCAAFLSATRDWPGTRRVLNISSGLGRRAMASQAAY
                     CAAKAGMDHFTRCLALEEARLPQGARVCSLAPGVIDTDMQVQLRGADAAAFPDRDSFE
                     QLKRGGALSSPADAARRVLAYLARPDFGQHPVADVREVLGA"
     gene            9122..>9617
                     /locus_tag="PK242_08320"
     CDS             9122..>9617
                     /locus_tag="PK242_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOZ94062.1"
                     /translation="MSREVVVVSGVRTAIGTFGGSLKDTPTVDLGALVVKEALARANV
                     DGKDVGHVVFGHVVNTEPRDMYLSRVAAINGGCAKETPAMNVNRLCGSGLQAIVSASQ
                     AILLGDCDIAIGGGAENMSRAPYASLATRFGARMGDAKMVDMMVGALHDPFHTIHMGV
                     TAENV"
BASE COUNT         1430 a         3586 c         3278 g         1323 t
ORIGIN      
        1 gcttcttcgc cccgcccacg gtgtggatcg cgctgctgcg ctcgccgctg ttcgatcaga
       61 ccgatctgag caagctggcc aagggctact acggcgcttc catcatgccg gtcgaggtgc
      121 tcaaggaaat cgcgcgccgc ctgcccgccg tgcgcctgtg gaacctgtac ggccagaccg
      181 aaatcgcgcc gctggccacc atgctgggcc ccgacgacca gctgcgcaaa cccggatcgg
      241 ccggccgcgc ggtgctgaac gtggagacgc gggtggtcga cgacgccctg cgcgacgtcg
      301 ccgtgggcga ggtgggcgaa atcgtgcacc gctcgccgca cctgatgctg ggctattttc
      361 acgacgacga gcgcacggcg gcggccttcg agggcgagtg gtttcactcg ggcgatctgg
      421 gcaccatcga cgccgagggc tacatcaccg tggtcgatcg caagaaggac atgatcaaga
      481 gcggcggcga gaacgtcgcc agccgcgagg tcgaggaaat gatctaccgc ctgcccgaag
      541 tgagcgaagt ggcggtgatc ggcctgcccg accccaagtg ggtcgaggcg gtgacggcgg
      601 tggtggtcgt caaggccggg caggagctga ccgaagcgca ggtcatcgcc cactgcaacg
      661 cacacatggc gaccttcaag gcgcccaagc gcgtgatctt caccgaccag ttgcccaaga
      721 accccagcgg caagctgctc aagcgccagt tgcgccagac cttcgtctga ccgccgcgcc
      781 ccgccggcga gtgtgcgaca gcggtgcccg tggtgcgcgc cgaccgggcg ccggggcctc
      841 cagaccttca agtctttctg gcccccggcg cgcacccatc aagcgcgagc agctatcgaa
      901 ttcatagtat tcaagcggtt cgcggggtgc cgcgcaggcg ctgaaacagc gtcaccgccg
      961 ccaccaccac cgcgccgacc agcatgccca cggcggcgtt gaagcccatg tcggccagca
     1021 cccgcagcac gccgcccagg ccagcggccc agccttccag caggtggtgc agcgccggca
     1081 cgccatgcac gatgatgccg ccgcctacca ggaacatggc ggccgtgccg gccaccgaca
     1141 gccccttcat cagccagggc gccagcgcca ggatgccgcg gcccagcgcc tgcgcgccgg
     1201 cgccggcgcg ccggctcagc cacaggccca ggtcgtccag cttgacgatg ccgcccacca
     1261 ggccatagac gcccaccgtc atcaggatcg cgataccgac cagcacggcc acctgggtcc
     1321 agaaatcggc ctgcgccacg gcgcccaggg tgatggcgat gatctcggcc gagaggatga
     1381 agtcggtgcg cacggcgccc ttgatcttgt ctttttccag cgccgccaga tcgacctgcg
     1441 ggtcggccag cgccgcctcc agctcggcct cgtgggcctg gtcctcggca cgggtatgca
     1501 gcagcttgtg cagcagcttc tcgcagccct cgaagcacag aaaggcgcca cccagcatca
     1561 acagcggcgt caccgcccag ggcgcaaagg cgctgacgag cagggccacc ggcaccagga
     1621 tggccttgtt gacgaacgac cctctggcca ccgcccacac caccggaatc tcgcggtggg
     1681 cgctgacgcc ggtcacctgc tgggcgttca gggccaggtc gtcgcccagc acgccggcgg
     1741 tcttctgcgc cgacaccttg gtcagcaccg ccacgtcgtc ggccatggcg gcgctcttgc
     1801 gcgcagcgat cttggacatg acggcgacgt cgtcgagcac ggtggcgatg tcgtcgagca
     1861 gggcgagcag gctggtggcc atgaaggctt tccgggaaaa ggcgaagaat gcggggtcgg
     1921 tacgtcgaac ccggggccgg caggccatcg ggcctggcac gccgcccgag cgccaacccc
     1981 tcggcaggcg gcgattatct ccgcgccgcg tgcccgccac ccgggcaagt cggcgcctga
     2041 aagccgtggg gtaccattgc aggttggccc tcgggccgca ccacagcctt cattgattcg
     2101 gcctttggat gtcttgaaca cctttcgcgc tgagcttgtc caagcccatc gacaggctca
     2161 ggttggacgg ttcagatttc aggattccga aaccctagga gaacacgccc atgcccttgc
     2221 tcctgacccg catcgccggc cgcgccctgg cctgcgtcgg tctgatgtgc gcggccgccg
     2281 ccatggccca ggcgcccgag gcgccggccg gtgacgcgcg ccagggcacc ttcaagacgg
     2341 tgcgcggcga cgtcaccgtg gtgcgcgaca acacccgcgt ggcggccatc gtgggtgcag
     2401 gcgtgcgatc gaccgaccgc atcctgagcg gcgccgaaag tgccgccgcc gtcacgctgc
     2461 tcgacggcac ggtgctggcc atcgggcccg acagcgcgct ggacctgagc cagtaccagt
     2521 tcaaccccac cacgcaggaa ggcagcgtgc tggtcaacct gatgcgcggc tcgctgcgca
     2581 tggtcaccgg gctgatcgcc aagctcaagc ccgagcaggt caaggtcacc acgccgacca
     2641 gcgtcatcgg cgtgcgcggc accgacttca tcgtggagga aaacccatga ggctggcccc
     2701 cttgctgacg gccgtggccc tgctggcggc cggttgcgca ccgaccaccc gcgtcaccct
     2761 gctgccgcag gccgaccacc cgcaggccgc ggtcgaggtc acggcgcgcc agcgcagcac
     2821 gcggctggcg cagccctacg cctcggcggc ggtgcgccag cagggcccgg agctggagca
     2881 actcgacgcc gccacggtgc acaagcgcta ccctgccctg ctggccgtga tgccaccgcc
     2941 ggccgagcgt ttcgagctga agttcatcac cggtggcact cgactgacgc ccgaatccac
     3001 cgccgaactg agcgatgtgc tggcgagcgc catcgcgcgc gcgggcggtg agatcgtcgt
     3061 catcggtcac accgaccggg tcggcacgct ggaggcgaac gacgcgcttt cgctgcgccg
     3121 cgccgaagcg gtgcgcgccc tgatcgtcga gcgcggcttc gaggcggcgc gtgtgcgcgc
     3181 cgtcgggcgc ggcgagcgcg aaccgcaggt gcccaccgag gacgacgtcg acgagccgcg
     3241 caaccggcgc gtcgagatcg tggtgcgctg agcatcaccg ccggccaccc atccttcgcg
     3301 catgcgcccg aagtcgcgaa cctggctgtt ggccggcgcg ctgctgctgg cccacacggt
     3361 gcaggcggcg ccgttccgtg acgacatggc gcagcgcacc caggcctgca ccgcctgcca
     3421 cggcgagcag ggccgcgccg gccccgacgg ctactacccg cgcctggccg gcaaaccggc
     3481 gggctacctg tacaaccagt tgctgaattt ccgcgacggc cggcgtcact acggcctgat
     3541 gacgcgcatg gtggacgtgc tgagcgacgc ctacctgatg gacatcgcgc gccacttcgc
     3601 cgccctgcag gtgccctatc cgcccccggc accgccggcc agcccgccgc cgccggcggt
     3661 gctcgaacgc ggccgcgcgc tggcgctgca gggcgatgcc gcccagggcg tgccggcctg
     3721 cgtgcaatgc cacggccgga cactgaccgg cgccctgccc gatgtgcccg gcctgctcgg
     3781 gctgccggcc gactacctga gcgcgcagct gggcggctgg cgcaccggcc agcggcgcgc
     3841 ccacgcgccc gactgcatgg cccgcatcgt gcagcgcctg tccgatcgcg atgcccacgc
     3901 cgtggtcagc tggctggcca gccagccggt gccggccgac ccccgccccg cccccgcacg
     3961 cccgcccctg cctgccgagt gggccgacct gcgctgtggc cgcgcccccg agccagaggt
     4021 cgccaggtga gcgcgctgcg ccgcctgctg ctggggctgg ccgggctggc gctgctgggg
     4081 gcgggcctgc tgtggcagat caacttcaac gacggtgtcg acaccaggtc cagcgcaccg
     4141 gtcgccagcg acgcggccac gcgcgcgcgc ggcgcctacc tggcccgggc aggcaactgc
     4201 ctggcctgcc acacggcgcg cggcggcccg gcggcggccg gcggccggcc catcgccacg
     4261 cccttcggca ccgtctacag cagcaacctg acgcccgatg cgcgcgccgg cattggcggc
     4321 tggaacgccg ccaccttctg gcgcgcgctg caccatggcc gctcggccga cgggcggctg
     4381 ctcaacccgg tgttccccta cacccacacc accgggctca cgcgcgccga cagcgacgcc
     4441 ctgttcagct acttcctcgg cctgccgccc agcgccaccg cggtgccggc ccacgagctg
     4501 cgctggccct tcggcaccca ggcggcgctg gcgctgtggc gcgcgctgta cttccggccc
     4561 ggcagctttc agcccgaccc ggcccgcagt gagcagctca accgcggcgc ctacctggtg
     4621 caggcggtgg ctcactgctc ggcctgccac accccgcgcg accgcctggg cggcgccgac
     4681 tggcagagcc tgagcggtgg cacggtgccc ggcaccggct ggtacgcgcc ctcgctgacg
     4741 gcacgcgacg aaggcgccgt ggggcactgg ccggccgagc agatcgtcca gctgctggcc
     4801 accggcgccg ccccacaagg ggtcgccagc gggccgatgg ccgaagtggt ctatcagggc
     4861 acgcagcacc tgagcggcgc cgatctgcag gccgtggcgg cctacctgca gcacctgccg
     4921 ccacagccgg gccggcagga cacgccggcg ccacgccagc gccccagcgc ggccggcgag
     4981 cggctgtacg ggcagcactg cgcggcctgc catggcgaag acggccaggg cgtgcctggc
     5041 gcctatcccc cgctgtccgg caaccgcagc gtcaccatgg ccaacaccca gaacctgctg
     5101 cagatcgtgc tgttcggcgg tttcggaccg gccaccgcgg gccgcccgca gcccttcggc
     5161 atgccgccgt tcgccctgac cctgagcgat gccgagatcg cggcggtgct gagctacatt
     5221 cgcggcgcct ggggcaatga tgccggcgaa gtggccccac tcgacgtggt gcgcatgcgc
     5281 cacgtcaacc attcgggtct tccgtgacat tggttacgct tgaaccgcaa cggcaacgat
     5341 ttgtgacggt tcgcgcccta tactgcggcg tcagccgtga cgcccccaca gcaccatccg
     5401 gtgcctgctc aggtcgggtt gcggcgcaaa cactggggag gaacgaacca ccatggcgct
     5461 gaactggctc atgtccaagc gggcccgtcc gcaaaccgag ggcgacggcg cccagcggcc
     5521 cgacggccgt gcggccgagg ctgaggccaa cgccgacgat caggagctgc gctggcccga
     5581 ggtggtgcgc catttcggtc aggaagtggc gatggcgctg aacaccgccg ccgaccagct
     5641 ggaacgcctg agccggctgg aacccagcct ggtgggcagc ctgcaaaccg tgaccgacgc
     5701 cgtcgagcgt gcacgccagg ccggcatggc cgcccagcaa gtgctgcgcc tgaccgaaga
     5761 cccgccggtg cagcatcgcg agttgctggg cctggacgac gtggtgcgcg ccgtgctgac
     5821 cacgcgcgcc gagtggctgc agcgtcacca ggtcggcgtg cggcatggcc tggcgcagac
     5881 tcaggtctat gccgactcca gcatgctgta catgctggtc gacgaactgc tgctgtgggc
     5941 agcccagatg gcaccccaga tcgccatcat ggtggaccgc tccagccgca gcgggcgccc
     6001 acgcctgagc gtgttggcct ggtgcgatcc ggcacaggtg cccgaggccg cctggcgcgg
     6061 catgcgctgg atgctgtggc accagctggc ccgctcgctg ggtgccagcg cgcgcgtgga
     6121 cacgcagacc gaccatgtgc gtgtcacgct cagcctgagc gccgtcaccg aggaacaact
     6181 ggccaccggg atcgaagaat cggtcggccc gtccagcgtg tcggcggtga tccagggctg
     6241 tcgcgtgctc atcgtctcgg gcaacgcgca acggcgcgcc cagagcctgc aggcgctgac
     6301 cggctacggc ctgattctcg acagcgcggg cgacatgacc gaggccgagc gcctggccac
     6361 gcggcacctg cccgacgccg tggtctacga cggcagcgtc aacccgagtg cggtcgcagg
     6421 tctgcgcgag agcctggagg cgcgcgctgg cacaccggcc gccttcatcg agattcacga
     6481 cgacgacagc gcctccgatt tccgcacctc caccgtcggt tcggtgtcca ccggccacat
     6541 cgccgccggc tcgctcaatc aggcgctggg cccggcgctg gtgttcgagc tgtgcaaggt
     6601 gatgtgatcc attgctattg ttttagatag cttgctgcgc agtctggata agcgcaaaac
     6661 cgatatttgg ctcatgtttt cggggtcacc cagcggcgca tgagcgcaca gcccaggcac
     6721 cctgccagcg cagccgccac aaccgccgat ccgccgctgc aggtgcgcta cgaagacgcc
     6781 cacctgatcg cgctcgacaa gccggccggc ctgctgaccg tgcccggtcg cgggaccgac
     6841 aagagcgact gcctgagccg ccgcgcccag gcgcgctggc ccgatgcgct cgtcgtgcac
     6901 cggctcgatc aggccaccag cggcctgctg ctgctggcgc gcgggcccgc gatgcagcgt
     6961 gcgctcagcc aggcctttgc ccagcgccag gtgcacaagc gctatgaggc gctggtgcac
     7021 ggccggctga ccctgccggc cgacgccacc gacggctggg cgcggatcga cctgccgctg
     7081 atcgtcgact ggccgcagcg cccgcgcagc aaggtcgatc acgtggccgg caagcccagc
     7141 cagacgcgct ggcgcccgct ggcgcatgac gcgcagaccc ggcgcacaag actggcgctg
     7201 gagccgctca ccggccgctc gcatcagttg cgtgtgcatc tgctggcgat cggccacccg
     7261 atcgtgggcg acaccctgta cggcccgccc gacggcgcgc cgcgcctgat gctgcacgcc
     7321 tgcgcgctgc ggctgacgca cccggtcacc ggccagccgc tcgcattgga cagcgcggcg
     7381 ccgttctgac gcacctgccg acggcccgcc cggcgcgcta cagtgtgagt cgcgggcgcg
     7441 atcccgcaga aaggttgatt gacatgacac gtaggaacct gcaaggcctg ggcctggcgc
     7501 tgtcgctggc gctgggcggc ccgtggctgt cgggctgcac catcaccagc acgccgcaat
     7561 cgcaggcggc gccggccgcc acccgcgccg ccatcgatgc ggcggtgaat gaaaccctgt
     7621 cgcgtctgta ccacctggtg ccaggcacgc aggacatggt gtcgcgcgcc gccggggtgc
     7681 tggtgtttcc ctccgtgctg ggcggctcct tcgtggtcgg cgccgaacat ggcaaggggg
     7741 cgctgcgcgt cggtggccag accaccagct actacagcac caccggcgca tcgatcggct
     7801 ttcaggccgg cggccagtcg cgctcggtga tctacgtgtt caacacgcgc gaggcgctgc
     7861 agagcttccg tgccagcaac ggctggaccg ccgacgccga cgccaccgtg gcgatcggca
     7921 acgtgggggc caacggccgc gtcgattcgc acaccgtgca gcagcccatc gccagcttcg
     7981 tgctgaccaa cgtcgggctg gaggccgggg tgtcggtcgg cggcagccgc atcgtgccga
     8041 tcgccctgcc ctgaggagct gcgcacgcgc accccttcat gcccgccttc tgatcgggcg
     8101 ccgcccaccg ccgtcccatg aaccgcaaac cgacccgtca gctgtacgtc ctcaccggcg
     8161 cttcgcgcgg catgggccgt gccatggcgc agcagttgct ggcccccggc caccagctgc
     8221 tgacgctgtc gcgccggccc gatgaagcgc tggccagcca ggccggtgcg gccggcgcca
     8281 cgctgaccca gtgggcggtc gacctgggcg acgccgcccc ggtggcggcg cggctggccg
     8341 actggctgcg cgcgcaggac ggcaaggccc tgaccagcgc caccctgatc aacaacgccg
     8401 gcgccatccc ggccatcgtg ccgctgcgcg acgccgaccc gccggcgctg gcggccgccc
     8461 tgcgcctggg cctggaagca ccgatgcagc tgtgcgccgc cttcctgtcg gccacgcgcg
     8521 actggcccgg cacgcgccgg gtgctcaaca tctcctccgg cctggggcgg cgcgccatgg
     8581 cctcgcaggc cgcctactgc gccgccaagg ccggcatgga ccacttcacg cgctgcctgg
     8641 cgctggaaga agcgcgcctg ccccagggag cgcgcgtgtg ttcgctggcg ccgggcgtga
     8701 tcgacaccga catgcaggtg cagttgcgcg gcgccgatgc ggcggccttc ccggaccgcg
     8761 acagcttcga gcagctcaag cgcggcggcg cgctgagctc gccggccgac gccgcgcgcc
     8821 gtgtgctggc ctacctggca cggcccgatt tcggccagca cccggtggcc gacgtgcgcg
     8881 aggtgctcgg agcctaggcc aagcgatcgc gcatgatctg aatcaaactg gcctctcgtc
     8941 cgcttcccgt aagcgccacc agctatcgtg tcgatagcat ctcgaagttt cccggggctg
     9001 cggcgcgccg gcgccgcccg gccaggcccg cccgaccgcc cacgcacgcc ccggtgcggc
     9061 atactgcctg cctgtcccga gctgtcgttt tttcattcca cccttgcaag gagcatccat
     9121 catgtcccgt gaagtcgtcg tcgtcagcgg tgtgcgcacc gccatcggta cctttggcgg
     9181 cagcctgaaa gacaccccca ccgtcgatct gggcgcgctg gtggtcaagg aggcgctggc
     9241 gcgcgccaac gtggacggca aggacgtggg ccatgtggtg ttcggccatg tcgtgaacac
     9301 cgagccgcgc gacatgtacc tctcgcgcgt ggcggccatc aacggcggct gcgccaagga
     9361 aacgccggcc atgaacgtca accgcctgtg cggctcgggc ctgcaggcca tcgtcagcgc
     9421 cagccaggcc atcttgctgg gcgactgcga catcgccatt ggcggcggcg ccgagaacat
     9481 gagccgcgcc ccctatgcca gcctggccac ccgctttggc gcgcgcatgg gcgacgccaa
     9541 gatggtggac atgatggtcg gggcgctgca cgaccccttc cacaccatcc acatgggcgt
     9601 gacggccgag aacgtgg
//
LOCUS       DAOOOG010000097         9495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_8408_length_9495_cov_25.849047, whole genome shotgun sequence.
ACCESSION   DAOOOG010000097 DAOOOG010000000
VERSION     DAOOOG010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8408_length_9495_cov_25.849047"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /gene="groL"
                     /locus_tag="PK242_08325"
     CDS             <1..1256
                     /gene="groL"
                     /locus_tag="PK242_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345520.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOZ94063.1"
                     /translation="KASKATTTSKEIAQVGSISANSDESIGKIIADAMDKVGKEGVIT
                     VEDGKSLDNELDVVEGMQFDRGYLSPYFINNPEKQAAILDNPYVLLFDKKISNIRELL
                     PTLEAVAKAGRPLLIIAEEVEGEALATLVVNTIRGILKVVAVKAPGFGDRRKAMLEDI
                     AILTGGKVIAEEVGLSLEKVTLADLGQAKTIEVGKENTTIIDGAGKAADIEARVKQIR
                     VQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVE
                     EGIVAGGGVALLRARQAAGAIKGANADQDAGIKLVLKAIESPLREIVINAGDEASVVV
                     NKVLEGKGNFGFNAANGQYGDMIEMGILDPTKVTRTALQNAASVAGLMLTTEVMVAEA
                     PKDDAPAGGGMPGGMGGMGGMDGMM"
     gene            complement(1440..3044)
                     /gene="pgi"
                     /locus_tag="PK242_08330"
     CDS             complement(1440..3044)
                     /gene="pgi"
                     /locus_tag="PK242_08330"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOZ94064.1"
                     /translation="MDWRERARGDQTAVWPRLAAHGRQRLGAAGHFDLRQAFAEDAQR
                     VERFSLEAPPLLADLSKNLIDAPAQQLLTELAQACGVAAHRDAMLAGERINTSEARPV
                     THVHWRWASNRALAPEEQAPEAIKNIASVVPYASATLADMLSYAESVRADRGITDVVH
                     IGIGGSDLGPRLVVQALRAAGQAGGPRLHFVANMDGHELASLLPRLTASRTLFIVVSK
                     TFGTAETLRNARSARDWFLAQGGSDVARHFVAVSANLAAARAFGAGRCFGFDDGLGGR
                     FSLWSPVGLAIAIAAGSAGFEALQAGARAMDRHFIQAPLARNLPVQLALLDLWYRNVL
                     GLASRCVAPYHHGLRSLPAYLQQLEMESNGKGVDLHGRPLAVASAPVTWGQAGSNGQH
                     AFFQMLHQGSDVVPVEFVLVARDAHGLPEHHAKLLASALAQSRALMLGQEGDAPERHF
                     PGNRPSTVLLLPDLSPHSLGALLALYEHRCFVLGSLWGINSFDQWGVELGKRHAGQIE
                     AALRGGALGPLDASTAALVAHLRGPA"
     gene            complement(3065..4012)
                     /gene="tal"
                     /locus_tag="PK242_08335"
     CDS             complement(3065..4012)
                     /gene="tal"
                     /locus_tag="PK242_08335"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOZ94065.1"
                     /translation="MNQLDALKQFTTVVADTGDFRQIAQYQPRDATTNPSLILKAVQK
                     PEYAPLLADTLARHRGKPLDEVVDRLLVRFGCEILSLIPGRVSTEVDARLSFDTEATV
                     TRAERLIELYQAEGVHIDRVLIKIASTWEGIRAAERLERRGIHCNLTLLFSFCQAVAC
                     GQARVQLISPFVGRIYDWYKKAAGSQWDEAAMAGPNDPGVRSVRRIYEHYKRFGIATE
                     VMGASFRNVGQITALAGCDLLTISPELMAQLAASPAPLARALDAEAARRLTLPAVQFD
                     EAGFRLALNQDAMATEKLAEGIRVFIQDAEKLEALMQAA"
     gene            complement(4086..4739)
                     /locus_tag="PK242_08340"
     CDS             complement(4086..4739)
                     /locus_tag="PK242_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor C family protein"
                     /protein_id="HOZ94066.1"
                     /translation="MKPSILQITLASTLLAGLAQAAPFQNGSFEIGPAPGGSFITLGP
                     GNTSITGWEVTGAGIDYIGGLWQPADGSRSLDLSATAAGGIQQTFDTVAGHNYQVTFS
                     LAGNPSTPPLVKTVQVLATGGVATTYTFDISLSSTADMRWATQTYNFTATGAATTLSF
                     TSLDNSAMGPALDNVVVTDLTPATAVTPVPTLSEWALVLLATALGALGLRGRRGRGH"
     gene            complement(4885..5979)
                     /locus_tag="PK242_08345"
     CDS             complement(4885..5979)
                     /locus_tag="PK242_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ94067.1"
                     /translation="MTSPRFGAPWPSAALQPALIDAGPAQPAAAPAGATQPLVQAEAL
                     ARTFDVSPPLLNRLLEGKPRQLLHAVDEASFSIQAGQTLALVGESGCGKSTVARVLVG
                     LYQPTRGRFVFKGQDAHAAFRGKDGHHLRRSIQMIFQDPYASLNPRWKVADIIAEPLH
                     EHGMAASRAQALERVGELLQSVGLAAADGVKYPHQFSGGQRQRISIARALATQPEFLV
                     CDEPTSALDVSVQAQVLNIMKELQRQRGLTYLFISHNLAVVRHVSDAVGVMYLGRIVE
                     LAPKAELFSAPRHPYTRMLLDAIPKMHDTGRARTPVQGEVPNPLNPPSGCTFHPRCPH
                     ANARCKSERPALLTLKGIQVACHAVQEGRL"
     gene            complement(5976..6950)
                     /locus_tag="PK242_08350"
     CDS             complement(5976..6950)
                     /locus_tag="PK242_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ94068.1"
                     /translation="MSLLEVRNLIVEFPNRRGTLRALDDVSFEIAPGEILGVVGESGA
                     GKSLTGASIIGLLEPPGRVASGQIVLEGQRIDQLSHEQMRHIRGRKIGAIFQDPLTSL
                     NPLYSVGRQLVETIQTHLPVSAAEARQRAIDLLKDTGIPAAEQRIDHYPHQFSGGMRQ
                     RVVIALALAAEPKLIVADEPTTALDVSIQAQIIGLLKRVCRERGAAVMLITHDMGVIA
                     ETCDRVAVMYAGRVAEIGPVHEVINHPAHPYTAGLMASIPDMEQDRETLNQIDGAMPR
                     LNAIPRGCAFNPRCPRVFERCRVERPDLLPAGATQAACWLHARQEVAA"
     gene            complement(7082..7993)
                     /locus_tag="PK242_08355"
     CDS             complement(7082..7993)
                     /locus_tag="PK242_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ94069.1"
                     /translation="MSNALARWWDSDVAYSFRSSPTAIVAALVGLVCVFCAVFAPWVA
                     PHNPLDLTTLELSDARLPPFWMEGGSSKYWLGTDDQGRDILSALMYGARISLVVGLAS
                     VLLSVLVGVGLGLIAGFRGGWVDGFLMRVCDVMLSFPAILIALLVAGVARAKFPDAGT
                     SVAFGVLIIAIALTGWVQYARTVRGSTMVERGKEYVQAARVTGVAPWRIMLKHVLPNV
                     MGPVMVLATIQVATAIITEATLSFLGVGVPPTSPSLGTLIRVGNDYLFSGEWWITIFP
                     GVMLVLIALSVNLLGDWLRDALNPRLR"
     gene            complement(8084..9064)
                     /locus_tag="PK242_08360"
     CDS             complement(8084..9064)
                     /locus_tag="PK242_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ94070.1"
                     /translation="MGAFILRRLLQAIVVMVSVGFIAFLLFQYVGDPVLILLGQDATP
                     DQIRQLRADLGLDQPFFVQFWHFLLNAVQGEFGLSLRQGAKVSRLLGERFPATFELAL
                     VAAALALLIGIPMGVYAALRRGSFLSQVFMTVSLLGVSLPTFLIGILLILIFSVWLGW
                     LPSFGRGETVRIGWWSSGLFTLDGWLHMILPALTLAIFQLTLIMRLVRAEMLEVLRTD
                     YIKFARARGLTDRAIHFGHALKNTLVPVMTITGLQLGGLIAFAIITETVFQWPGMGLL
                     FIQAVTFADVPVMAAYLCLIALIFVVINLVVDLLYVVVDPRLRVAKAAGH"
     gene            complement(9259..>9495)
                     /locus_tag="PK242_08365"
     CDS             complement(9259..>9495)
                     /locus_tag="PK242_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94071.1"
                     /translation="SYSDNHKVRRGFSIGGTATFGAASVTLDLTRDTKHPVKKYTNGL
                     LEGKYALSKRTFLYAAYLRLDGNNNYGLGIRHNF"
BASE COUNT         1632 a         3169 c         3333 g         1361 t
ORIGIN      
        1 agaaggccag caaggccacc accaccagca aggaaatcgc ccaggtcggc tcgatttcgg
       61 ccaacagcga cgagtcgatc ggcaagatca tcgccgacgc catggacaag gtgggcaagg
      121 aaggcgtgat caccgtcgaa gacggcaaga gcctggacaa cgagctggac gtggtcgaag
      181 gcatgcagtt cgaccgcggc tacctgtcgc cctacttcat caacaacccc gagaagcagg
      241 ccgccatcct ggacaacccc tacgtgctgc tgttcgacaa gaagatcagc aacatccgcg
      301 agctgctgcc cacgctggaa gccgtggcca aggccggccg tccgctgctg atcatcgccg
      361 aagaggttga gggcgaagcc ctggccaccc tggtggtcaa caccatccgc ggcatcctga
      421 aggtcgtggc cgtcaaggcg cctggcttcg gcgaccgccg caaggccatg ctggaagaca
      481 tcgccatcct gaccggcggc aaggtgatcg ccgaggaagt gggcctgtcg ctggagaagg
      541 tgacgctggc cgacctgggc caggccaaga ccatcgaagt gggcaaggaa aacaccacca
      601 tcatcgacgg cgccggcaag gccgccgaca tcgaggcgcg cgtcaagcag atccgcgtgc
      661 agatcgagga agccaccagc gactacgacc gcgagaagct gcaagagcgc gtggccaagc
      721 tggccggcgg cgtggccgtg atcaaggtcg gtgctgccac cgaagtcgag atgaaggaga
      781 agaaagcccg cgtggaagac gccctgcacg ccacccgcgc tgcggtggaa gaaggcatcg
      841 tcgccggcgg tggcgtggcc ctgctgcgcg cccgccaggc ggccggcgcc atcaagggtg
      901 ccaacgccga ccaggacgcc ggcatcaagc tggtgctcaa ggcgatcgag tcgccgctgc
      961 gtgaaatcgt catcaacgcc ggcgacgaag ccagcgtggt ggtcaacaag gtgctggaag
     1021 gcaagggcaa cttcggcttc aacgccgcca acggccagta cggtgacatg atcgagatgg
     1081 gcatcctgga cccgaccaag gtcacccgca ccgcgctgca gaacgccgcc tccgtggccg
     1141 gcctgatgct gaccaccgag gtgatggtcg ccgaagcgcc gaaggacgac gccccggccg
     1201 gcggcggcat gccgggtggc atgggcggca tgggcggcat ggacgggatg atgtgatcgt
     1261 cccccgaccg gcctgaagcc tgtgggtggg caacggcgtt gcccacccga caagccaagc
     1321 cgctggagac agcggctttt tttttggggc ctgagccgcg caactgcggt gcaggcggtg
     1381 cggcgggcgc aggcggcggc cggatcagca gcggcacacc cccgccggcg ccgaccgctt
     1441 cacgccggac cgcgcaggtg cgccaccaga gccgccgtcg acgcatccag gggccccaac
     1501 gcaccgccac gcagcgccgc ctcgatctgg ccggcgtggc gcttgcccag ttccaccccc
     1561 cactggtcga agctgttgat gccccacagc gagcccagca cgaagcagcg gtgctcgtac
     1621 agcgccagca gcgcgcccag gctgtgcggc gacaggtcgg gcagcagcag cacggtggac
     1681 ggccggttgc cggggaagtg gcgctccggc gcatcgccct cctgccccag catcagcgca
     1741 cgggactgcg ccagcgcgct ggccagcagc ttggcgtggt gctccggcag gccatgggcg
     1801 tcgcgcgcca ccagcacgaa ttccaccggc accacgtcgc tgccctggtg cagcatctga
     1861 aagaacgcat gctgcccgtt gctgccagcc tgcccccagg tgacgggggc gctggccacg
     1921 gccagggggc ggccgtgcaa atcgacgccc ttgccgttgc tttccatctc cagctgctgc
     1981 aggtaggcgg gcaggctgcg caggccgtgg tggtagggcg ccacgcagcg gctggccagg
     2041 ccgaggacgt tgcgatacca caggtccagc agcgccagtt gcaccggcag gttgcgcgcc
     2101 agcggcgcct ggatgaaatg ccggtccatg gcgcgggcgc cggcctgcag ggcctcgaag
     2161 ccggcgctgc cggcagcgat ggcgatcgcc agaccgaccg gcgaccagag cgaaaagcgc
     2221 ccgcccaggc cgtcgtcgaa accgaagcag cgcccggccc cgaaggcgcg cgcggccgcc
     2281 aggttggcgc tgacggcgac gaagtggcgc gccacgtcgc tgccgccctg ggccaggaac
     2341 cagtcgcgcg ccgaccgcgc gttgcgcagg gtttcggcgg tgccgaaggt cttggagacg
     2401 acgatgaaca gcgtgcgcga agcggtcagc cgcggcagca ggctggccag ctcgtgcccg
     2461 tccatgttgg caacgaagtg caggcgcggg ccaccggcct gtccggcggc gcgcagcgcc
     2521 tgcaccacca ggcgcggccc caggtcggag ccgccgatgc cgatgtgaac cacgtcggtg
     2581 ataccgcggt cggcacgcac ggactcggca tagctcagca tgtcggccag cgtggcgctc
     2641 gcataaggca ccaccgatgc tatgttttta atagcttctg gcgcttgctc ctcgggcgcc
     2701 agagcacgat ttgatgccca acgccagtgc acgtgggtga ccgggcgcgc ttcgctggtg
     2761 ttgatgcgct cgccggccag catggcgtcg cggtgcgccg ccacgccgca ggcctgcgcc
     2821 agctcggtca gcaactgctg cgccggggcg tcgatcaggt tcttcgacag atcggccagc
     2881 agcggcggcg cctcgaggct gaaacgctcg acgcgctgcg cgtcctcggc gaaggcctgc
     2941 cgcagatcga agtgcccggc agcgcccaag cgctggcgcc catgcgccgc cagccgcggc
     3001 cacaccgccg tctggtcgcc gcgcgcgcgt tctcgccagt ccatcggcac aggtcggggc
     3061 ggggtcaggc cgcctgcatc agggcttcga gcttttcggc gtcctggatg aacacgcgga
     3121 tgccctcggc cagcttttcg gtggccatgg cgtcctgatt gagcgccagg cgaaagcccg
     3181 cttcgtcaaa ctgcacggcc ggcagcgtga gccggcgtgc cgcctcggcg tccagcgcgc
     3241 gcgccagcgg cgccgggctg gcggccagtt gcgccatcag ttcggggctg atggtgagca
     3301 gatcgcagcc ggccagcgcg gtgatctggc cgacgttgcg aaagctcgcc cccatcacct
     3361 cggtggcaat gccgaagcgc ttgtagtgtt cgtagatgcg gcgcaccgac cgcacgcccg
     3421 ggtcgttcgg gccggccatg gcggcttcgt cccactggct gccggcggcc ttcttgtacc
     3481 agtcgtagat gcggccgacg aagggcgaaa tcagttgcac ccgggcctga ccgcaggcca
     3541 ccgcctggca gaacgagaac agcagcgtca ggttgcaatg gatgccgcgc cgctccagcc
     3601 gctcggcggc gcggatgcct tcccaggtgc tggcaatctt gatcagcacg cggtcgatgt
     3661 gcacgccctc ggcctggtac agctcgatca gccgctcggc gcgcgtcacc gtggcttcgg
     3721 tgtcgaacga caggcgcgca tcgacctcgg tcgacacgcg gcccgggatg agcgacagga
     3781 tttcgcagcc aaagcgcacc agcagccgat cgaccacttc gtccagcggc ttaccgcggt
     3841 gacgcgccag ggtgtcggcc agcagcggcg cgtactcggg cttttgcacc gccttcagga
     3901 tcagcgacgg gttggtggtg gcgtcgcgcg gctgatactg cgcgatctgc cggaagtcgc
     3961 cggtgtcggc caccaccgtg gtgaattgct tgagggcgtc gagctggttc atggtggggc
     4021 gattatcccc gctcgccccg agcgacgaca cgggccacca ccgacgcggc ggcggcccgc
     4081 caggctcagt gaccgcgccc gcggcggccg cgcaggccca gcgcgcccag cgccgtggcc
     4141 agcagcacca acgcccattc cgacagggtg ggcaccgggg tgaccgcggt ggccggggtg
     4201 aggtcggtca ccaccacgtt gtccagcgcc ggtcccatgg ccgaattgtc gaggctggtg
     4261 aagctcagcg tggtggctgc gccggtcgcc gtgaagttgt aggtctgcgt ggcccagcgc
     4321 atgtcggccg tgctggacag gctgatgtcg aaggtgtagg tggtggccac gccccccgtg
     4381 gccagcacct ggacggtctt gaccagcggc ggtgtggacg ggttgccagc cagggagaac
     4441 gtcacctggt agttgtggcc ggccaccgtg tcgaaggtct gctgaatgcc gccagccgcg
     4501 gtggcgctga ggtccagcga gcggctgccg tcggccggct gccacaggcc accgatgtag
     4561 tcgatgccgg cacccgtgac ttcccagccg gtgatgctgg tgttgccggg gcccagcgtg
     4621 atgaacgacc caccgggcgc gggcccgatt tcaaaactgc cgttctggaa gggcgcggcc
     4681 tgcgccaggc ccgccagcag cgtgctggcc agggtgatct gcagaatcga tggcttcatg
     4741 acaatcttcg atgatgaggg ttgacacatc ccactgtggc caccgaccga gcgttcgggg
     4801 tcgcggcggg gcttgcggat tggaacataa aacgctgcag gcgaccaccc cgtgtgcgcc
     4861 agcagctaca gtttgcagag cggatcagag tcgcccctct tgcaccgcgt ggcaggccac
     4921 ctgaatgccc ttgagcgtca gcagggccgg tcgctcgctc ttgcagcgcg cattggcgtg
     4981 cgggcagcgc gggtggaagg tgcaaccgct gggcgggttg agcgggttgg gcacttcgcc
     5041 ctgcaccggg gtgcgggcgc ggccggtgtc gtgcatcttg gggatggcgt ccagcagcat
     5101 gcgcgtgtag gggtggcgcg gcgcgctgaa cagctcggcc ttgggtgcca gctcgacgat
     5161 gcggcccagg tacatcacgc ccacggcgtc gctgacgtgg cgcaccaccg ccaggttgtg
     5221 gctgatgaac aggtaggtca ggccacgctg gcgctgcagc tccttcatga tgttgagcac
     5281 ctgggcctgc accgacacgt cgagcgcgct ggtgggttcg tcgcagacca ggaattcggg
     5341 ctgggtcgcc agggcgcgcg cgatcgagat gcgctggcgc tggccgccgg agaattggtg
     5401 cgggtacttt acgccatcgg ccgccgccag gccgaccgat tgcagcagct cgcccacgcg
     5461 ctccagcgcc tgcgccctgg acgcggccat gccgtgctcg tgcagcggct cggcgatgat
     5521 gtcggccacc ttccagcgcg ggttgaggct ggcgtacggg tcctggaaga tcatctggat
     5581 gctgcgccgc aggtggtgcc cgtccttgcc ccggaaggcg gcgtgcgcgt cctgcccctt
     5641 gaagacaaag cgcccgcgcg tgggctggta caggcccacc agcacgcgcg ccacggtgct
     5701 cttgccgcag cccgattcgc ccaccagcgc cagcgtctgg ccggcctgga tgctgaagct
     5761 ggcttcgtcc accgcgtgca gcaactggcg cggcttgcct tcgagcaggc ggttgagcag
     5821 cggcggcgag acgtcgaagg tgcgcgccag ggcctcggcc tgcaccagcg gctgggtcgc
     5881 gcccgccggc gccgcagcgg gctgggcggg gccggcgtcg atcagggcgg gctgcagcgc
     5941 ggccgacggc cacggtgcac cgaaacgtgg gctcgtcatg ccgccacctc ctgccgcgca
     6001 tgcagccagc acgcggcctg ggtggcgccg gccggcagca gatcgggccg ctcgacgcgg
     6061 cagcgctcga acacgcgcgg gcagcgcgga ttgaaggcgc agccgcgcgg aatggcgttc
     6121 aggcgcggca tggcgccgtc gatctggttg agcgtttcgc ggtcctgttc catgtcggga
     6181 atggaggcca tcagccccgc cgtgtagggg tgggccgggt ggttgatgac ttcatgcacc
     6241 ggcccgattt cggccacccg gccggcgtac atgacggcca cgcggtcgca ggtttcggcg
     6301 atcacgccca tgtcgtgcgt gatcagcatc accgcggcgc cgcgctcgcg gcagacgcgc
     6361 ttgagcaggc cgatgatctg cgcctggatc gacacgtcga gcgccgtggt gggttcgtcg
     6421 gccacgatca gcttgggctc ggccgccagc gccagggcga tcaccacgcg ctggcgcatg
     6481 ccgccggaaa actggtgcgg gtagtggtcg atgcgctgct cggcggcggg aatgccggtg
     6541 tccttgagca ggtcgatggc gcgctggcgc gcttcggcgg cgctcaccgg caggtgggtc
     6601 tggatggttt cgaccagctg ccggccgaca ctgtagagcg ggttgaggga ggtcagcggg
     6661 tcctggaaga tggcgccgat cttgcgccca cggatgtgac gcatctgctc atgcgagagc
     6721 tggtcgatgc gctggccttc cagcacgatc tgtcccgaag cgacacgccc cggcggctcc
     6781 agcaggccga tgatggaggc gccggtgagc gacttgcccg cgcccgattc gcccaccacg
     6841 cccaggatct cgcccggcgc gatctcgaac gacacatcgt ccagcgcacg cagcgtgccg
     6901 cgccggttgg gaaactcaac gatcaggttg cggacttcaa gcagtgacat agatgtgggt
     6961 tcggcctcgg agacacatga cccatgacgg cgtcgctacg cgacagcgca tgacaggatc
     7021 cgagacgcct ttgtcattca atacggaagc cgcgccagcg gcaaagccat gggtcatttc
     7081 gtcaccgcaa ccgcggattg agcgcatcgc gcagccagtc gcccagcagg ttgaccgaca
     7141 gcgcgatcag caccagcatg acaccgggaa agatggtgat ccaccattcg cccgaaaaca
     7201 ggtagtcgtt gccgacgcgg atgagcgtgc ccagcgaggg cgaggtgggc ggcacgccga
     7261 cacccaggaa ggacagcgtg gcctcggtga tgatggcggt ggccacctgg atggtggcca
     7321 gcaccatcac cggccccatg acgttgggca gcacgtgctt gagcatgatg cgccagggcg
     7381 ccacgccggt cacacgcgcg gcctgcacgt attccttgcc gcgctcgacc atggtgctgc
     7441 cgcgcaccgt gcgcgcgtac tgcacccagc cggtcagcgc gatggcgatg atcagcacgc
     7501 caaacgccac cgaagtgccg gcgtccggaa acttggcccg cgccacgccg gccaccagca
     7561 aggcgatcag gatggctgga aacgacagca tgacgtcgca cacgcgcatc agaaagccgt
     7621 ccacccagcc gccgcggaag ccggcgatca gccccaggcc gacgcccacc agcaccgaca
     7681 gcaggaccga ggccagcccc accaccagcg aaatgcgcgc gccgtacatc agcgccgaca
     7741 ggatgtcgcg cccctgatcg tccgtgccca gccagtactt gctgctgcca ccttccatcc
     7801 agaacggcgg cagacgggcg tcggacagct ccagcgtggt caggtccagc ggattgtgcg
     7861 gcgccaccca gggcgcgaac acggcgcaga acacgcatac cagcccgacc agcgccgcca
     7921 cgatggcggt gggtgagctg cggaagctgt aggcgacatc gctgtcccac cagcgggcaa
     7981 gtgcattgct catgaaatga tagctttctg cgctgtctgg gcgggcgctg caggcttgtt
     8041 tttcatatga tttcgagtgg cacgaggtgg cgcgggcggc gcctcaatgg ccggccgcct
     8101 tggccacgcg caggcgcggg tcgaccacca cgtacagcag gtccaccacc aggttgatga
     8161 ccacgaagat cagcgcgatc agacacagat aggccgccat caccggcacg tcggcgaagg
     8221 tgaccgcctg gatgaacaac aggcccatgc ccggccactg aaacaccgtc tcggtgatga
     8281 tggcaaaggc gatcaggccg cccagctgca gaccggtgat ggtcatcacc ggcaccaggg
     8341 tgttcttgag cgcgtggccg aaatggatgg cgcggtcggt cagcccgcgc gcgcgcgcga
     8401 acttgatgta gtcggtgcgc agcacctcca gcatctcggc gcgcaccagg cgcatgatga
     8461 gcgtgagctg gaagatcgcc agcgtcagcg ccggcaggat catgtgcagc cagccatcga
     8521 gcgtgaacaa acccgagctc caccagccga tgcgcaccgt ctcgccgcgc ccgaaactgg
     8581 gcagccagcc cagccagacc gaaaagatca ggatcagcag gatgccgatc aggaaggtcg
     8641 gcagcgacac ccccagcagc gacaccgtca tgaacacctg gctcagaaag ctgccgcgcc
     8701 gcagcgccgc atacacgccc atcggaatac cgatcagcag ggccagcgcg gcagccacca
     8761 gggccagctc gaaggtggcc ggaaagcgct cgcccagcag gcgcgacacc ttggcgccct
     8821 ggcgcagcga caggccgaac tcaccctgca ccgcattcag caggaagtgc cagaactgca
     8881 cgaagaacgg ctggtccagg cccaggtcgg cgcgcagctg gcggatctga tcgggcgtgg
     8941 cgtcctgacc cagcaggatc agcaccgggt cgcccacgta ctgaaacagc aggaaggcga
     9001 tgaagcccac cgagaccatc accacgatgg cctgcagcag tcggcggagt atgaaggcgc
     9061 ccatgggtca cgtgcagagt cgatggcggc atcgtagcaa accgggacca gcgccgatag
     9121 ggggcatacg ccaatggtca gaccagcgcg ttccggcatg acctggagga ccggactggc
     9181 gcaggcgggc acgaaaaagc cgcccgaagg cggcttttcg attcgaatca tgggctgccg
     9241 gttcaggcag cgtcacgatc agaagttgtg gcgaatgccc agaccgtagt tgttgttgcc
     9301 gtccagacgc aggtaggcgg cgtacaggaa ggtgcgcttg gacagggcgt acttgccttc
     9361 cagcagaccg ttggtgtact tcttgaccgg gtgcttggtg tcgcgcgtca ggtccagcgt
     9421 gacggaagcg gcgccgaagg tggcggtgcc accgatgctg aagccgcggc gcactttgtg
     9481 gttgtcgctg tagga
//
LOCUS       DAOOOG010000098         9448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_8465_length_9448_cov_28.777920, whole genome shotgun sequence.
ACCESSION   DAOOOG010000098 DAOOOG010000000
VERSION     DAOOOG010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8465_length_9448_cov_28.777920"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..662)
                     /gene="ruvA"
                     /locus_tag="PK242_08370"
     CDS             complement(84..662)
                     /gene="ruvA"
                     /locus_tag="PK242_08370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOZ94072.1"
                     /translation="MIGRLAGLLAEKTPPQILIDCQGVGYEVWVPMSSFYQLPALGER
                     VTLLTHFVVREDAQLLYGFVTETERAAFRELIRISGIGPRMALAVLSGMSVAELAQAI
                     AAQQAGRLTKVPGIGRKTAERLLLELKGRLGPDLGAPGAAAPASDSQTDILQALLALG
                     YSDKEAAAALQALPPDVGVSEGIRLALKALAK"
     gene            complement(854..1513)
                     /locus_tag="PK242_08375"
     CDS             complement(854..1513)
                     /locus_tag="PK242_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ94073.1"
                     /translation="MKAVLVIEDLPDIRSWLVDTVRVAFPDAEVTAIGRCGDGIDALA
                     RQPFDVVLTDLELPDGSGVSVIRALRQQQPMALSVVVTIHDDDEHLFPALQAGAFGYL
                     LKEASRQELVAQLQRIADGEPPLSPPIARRMLAYFSQGGPRVVDVTRFDPHEVALNGR
                     ETDILQRVAKGYTLPEIAGQLNLSRHTVADYVKQIYRKLNVSSRAEAALEAARRGLVR
                     P"
     gene            complement(1647..4001)
                     /locus_tag="PK242_08380"
     CDS             complement(1647..4001)
                     /locus_tag="PK242_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94074.1"
                     /translation="MNPSDPSPTLPSALYTESDLPPTLPGLLDTESGLSGSWRTPLDQ
                     LDLLERERPAQATPWLYRQAILLAGLWAVLLTTLALALATRQPWLGLRLAAQDGQVQV
                     VDVVPGSPAAVAGVHVGAQLQALATADGTQAMTLQLDDLRANPDELASYADWGRFYER
                     QDRLARLLGDASLRLTMVAHGAQEEALLLRPAPTRPPGSLPPLFWTLMVLQAAIMLMA
                     GWVITVYPPQPAAPRSAVGAMAISLVLAGVAVFGTRELALPSTLFHALVVLQHAGSLV
                     AALAVASILSHYPSRLPEMGGWRVWVVAGLFLLWALADAGWWLPSPNWASRAMLLAAV
                     VVVTVQAVRQWQLHSGGDSVARDEARTQALIWIPAAFCLFGLREGALLLGWAPPLSQA
                     WLYGLVVVAVAGRAVTHREVRVFELDDWTRQVLLTWGAGVTILLCYRLLQANRWLSDE
                     LAMLVSVLGLGLVYVLLTRRIWGRGILRRSQTQRELTSAILALGLTAPEDRGPLWMAL
                     LENTFEARVSLADGAQPYRVTTVLDGGRTLWVPPVAGLPGVLLGQRDRGRPLFARSDR
                     RMAARLSALAAQIIHARDAYVRGASEERLRIADDLHDDLGAKLLSLAHAGEQGEAGAD
                     VARLAREALEEMRLSVRHLKAQPVPAADVLADWRAETVARLSAAGVQVDWDAQLSGRV
                     APLPMRTVAHLTRVLREAVSNIIHHSGASYCRVLLQLSPTALHLDVEDNGQGMGLPGA
                     RGGAGGLSSIERRVRRLGGTHRFGSGALGGTLLMVRVPLESPHS"
     gene            4027..4986
                     /locus_tag="PK242_08385"
     CDS             4027..4986
                     /locus_tag="PK242_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOZ94075.1"
                     /translation="MILRHTFTPSNNTRLAHLCGPTDEHLRTIEAGLQVSIAHRHEQF
                     RIDGARKSAERALTVLQALYELADRPIRTETVQLMLAGDGEPLAEDAGGAHRLVTRRG
                     DLKARTPNQSVYLEHIAQHDITFGIGPAGTGKTYLAVACAVDALERQSVQRIVLTRPA
                     VEAGERLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVHKAFERQQIEIAPLAFMRGR
                     TLNHAFVILDEAQNTTPEQMKMFLTRIGFGSKCVVTGDVSQIDLPKGATSGLIDAERV
                     LRRVKGLAFTRFTSADVVRHPLVARIVDAYDAAGRPARTPKNS"
     gene            5045..5512
                     /gene="ybeY"
                     /locus_tag="PK242_08390"
     CDS             5045..5512
                     /gene="ybeY"
                     /locus_tag="PK242_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOZ94076.1"
                     /translation="MATPSLRLSLQFARSPEMAPHRAALPRAQVLRWLRAALGADVRL
                     AEIAVRVVGADEGRQLNRDYRGKDYATNVLTFDYSVAPQLAADLVLCAPVVAREAAEL
                     ATPLAQHYAHLLVHGALHAQGWDHETSEQEAEAMEARETAILAALGVPDPYAR"
     gene            complement(5653..6924)
                     /locus_tag="PK242_08395"
     CDS             complement(5653..6924)
                     /locus_tag="PK242_08395"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HOZ94077.1"
                     /translation="MKLFRSIQRARGAAAAVGAALLLLTTSAHADFVNGGFEQSPDFT
                     GWTTPGFAIPGAIPTFPPTQWSHLGLTTGTSQSAVLNGPGNAAATDGNLTWTNRVARV
                     HEEFSNGDGNTASAIEQVLTVAAGDIEADGKVHIRFNAAPVLEDPGHAPDEQPYFFIE
                     VTNQHGTSLYHTFNYANEPGVPWVLNSAGSHRYTNWQAFDIPVTAAVGDQLTLRLLAA
                     GCGQGGHAGALYVNDVRTQAAVQGPSLWVSAAGPATICLNRGGTTNVTYTYTYRNNGT
                     DPMYGVTASVSMPVTSDSTPVTTTFVSITNPTFGGGTCTAPASPGNPATCSIGTLQPG
                     QQGTFQMTVQIPAGAIGPTLNNGTYSLSGATSAQGGTVVTQLGPLVRTAMSENCAVNV
                     APVPTLGEWAMMLLASLMAAFGWVMMRRRMG"
     gene            complement(7148..7615)
                     /gene="dtd"
                     /locus_tag="PK242_08400"
     CDS             complement(7148..7615)
                     /gene="dtd"
                     /locus_tag="PK242_08400"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOZ94078.1"
                     /translation="MQALIQRVSQASVSVAGRVIGAIGPGLLVLLCAERGDDEAVADK
                     LLAKLLKLRIFSDAAGKMNLSVQDVAGGLLVVSQFTLAADTAGGNRPSFTGAAAPEDG
                     RRLYEHFVRQARAAHPQVATGEFAADMQVALVNDGPVTIPLRVAPARPAVGHG"
     gene            complement(7691..8650)
                     /locus_tag="PK242_08405"
     CDS             complement(7691..8650)
                     /locus_tag="PK242_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOZ94079.1"
                     /translation="MGTLSAWLTPRHLMAASIGVALLTIALKAAAWWVTGSVGLLSDA
                     LESFVNLAAASFGFLMVTVAEQPPDAGHPFGHDKAEYFSSGFEGILIIGAALAIVWAA
                     LPRFWWPQPLEQLGWGMGLALASALLNGGLALVMLRASRRHRSIALEADARHLLTDVW
                     TTGGVIAGLLLAGATGWLWLDPLVAIGVALNIAREGVRLMRRSSQGLMDAAVEPDTQA
                     RIDAVLAQFAARHGGPAAVLFDHVLTRKAGQRRFVNLHMHLPPGWTLARADALRGEVE
                     QALLAAVPGLHASIQMLPDGVEPISTELGAAPAEAQRPSRDEP"
     gene            complement(8742..>9448)
                     /gene="tyrS"
                     /locus_tag="PK242_08410"
     CDS             complement(8742..>9448)
                     /gene="tyrS"
                     /locus_tag="PK242_08410"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOZ94080.1"
                     /translation="ISVHEFLYPLMQGYDSVALKSDIELGGTDQKFNLLMGRHLQQEY
                     GQEPQCILTMPLLEGLDGVEKMSKSKNNYIGITEDANTMFAKVLSISDALMWRWYTLL
                     SFRSMAEIERLQAEVAAGRNPKEAKVLLAREITTRFHSAQAADAAEQDFAHRSRGGVP
                     DAIPEVQLSGAPLGIGVLLKQAGLAPSTSEANRLIDGGGVRVDASVVSDKALKLAAGS
                     YVVQVGKRRFARVTLA"
BASE COUNT         1502 a         3418 c         3230 g         1298 t
ORIGIN      
        1 gcggcagcgc ccgacaccag gcgcggcgcc gcggcgccgg gcggcagggc aaagtcgtcg
       61 gtgtggatgc tcatgcggcg tgattatttc gccaacgcct tgagcgccag ccggatgccc
      121 tcgctcacgc ccacgtcggg cggcagcgcc tgcagggcgg cggcggcttc cttgtcgctg
      181 tagcccagcg ccagcagcgc ctgcaggatg tcggtctggc tgtcgctggc gggggcggcg
      241 gcgccgggcg cacccaggtc ggggccgagc cggcccttga gctccagcag cagccgctcg
      301 gcggtcttcc tgccgatgcc cggcaccttg gtcaggcggc ccgcctgctg cgccgcgatg
      361 gcctgcgcca gctcggccac gctcataccc gacagcaccg ccagcgccat gcgcgggccg
      421 atgccgctga tgcggatcag ctcgcggaag gcggcccgct cggtctcggt gacgaagccg
      481 tagagcagct gcgcgtcctc gcgcaccacg aaatgcgtca gcagcgtgac ccgctcaccc
      541 agcgccggca gctggtagaa gctgctcatg ggcacccaca cctcgtagcc gacgccctgg
      601 cagtcgatca ggatctgggg tggggttttc tcggccagca ggccggcaag gcgtccgatc
      661 acggcggggg ctttcagaag atccacagag cgcgaccacc acgcccagcg tcaatgataa
      721 atcagccgaa acaagccgtc cggcctgcgc cagcagctct catttcaaga gcattcggga
      781 accacaggga gcgccggaac gcgccccacc gaaacctgcg gtgacaggtg gcaggcgggc
      841 accgggcaac cagtcagggc cgcaccaggc cgcggcgcgc ggcctccagt gccgcttcgg
      901 cgcgcgagga cacgttgagc ttgcggtaga tctgcttgac gtagtcggcc accgtgtggc
      961 gcgacaggtt cagctggccc gcgatttcgg gcagggtgta gcccttggcc acgcgctgca
     1021 ggatgtcggt ctcgcgtccg ttcagcgcca cttcgtgagg gtcgaaacgg gtcacgtcga
     1081 ccacgcgcgg gccgccctgg ctgaagtagg ccagcatgcg ccgggcgatc ggaggcgaca
     1141 gcggcggctc gccgtcggcg atgcgctgca gctgcgccac cagctcctgg cgcgacgctt
     1201 ccttcagcag gtagccgaag gcgccggcct gcagcgcggg aaacagatgt tcgtcgtcgt
     1261 cgtggatggt gaccaccacc gacaacgcca tgggctgctg ctggcgcagc gcccggatca
     1321 ccgagacgcc gctgccgtcg ggcagttcca gatcggtcag aacgacgtcg aacggctgcc
     1381 gcgccagcgc atcgatgcca tcaccacaac ggcctatcgc cgtcacctcg gcatcgggga
     1441 acgcaacgcg cacagtgtcc accagccagg aacggatgtc cggcagatcc tcgatcacaa
     1501 gcacggcttt catgacttgg ccatgctaag ccggcggcgg ccggatgctt cggcccggca
     1561 tccacgaact tgcgggaaat gcggaatatg tcgccaccgg cgcaccgcca ccgccctggg
     1621 ccggcttgcg gcagaggccg cgagagtcag ctgtgcggcg actccagcgg cacccgcacc
     1681 atcagcagcg tgccgcccaa tgcgccgctg ccgaagcggt gcgtgccgcc caggcggcgc
     1741 acgcggcgct cgatgctcga caggccgccg gcgccgcccc gagctcccgg caaccccatg
     1801 ccctggccgt tgtcttccac gtccaggtgg agggcggtcg gactgagctg caacagcacg
     1861 cggcaataac tggcaccgct gtggtgaatg atgttggaca cggcctcgcg cagcacccgc
     1921 gtcaagtggg cgacggtgcg catgggcagc ggggccaccc gccccgacag ctgggcgtcc
     1981 cagtcgacct gcacgccggc ggcggacagc cgcgcaacgg tttcagcgcg ccagtcggcc
     2041 agcacgtcgg ccgccggcac cggctgcgcc ttcaggtggc gcaccgacag ccgcatttcc
     2101 tccagcgcct cgcgcgccaa ccgggccacg tcggcgccgg cctcgccctg ttcgcccgcg
     2161 tgcgccagcg acagcagctt ggcccccagg tcgtcgtgca ggtcgtcggc gatgcgcagg
     2221 cgctcttcgc tggcgccgcg cacgtaggcg tcgcgggcgt ggatgatctg cgccgccaac
     2281 gccgacaacc gggccgccat gcgccggtcg ctgcgggcga acagcggccg gccgcggtcg
     2341 cgctgaccca gcagcacccc cggcagaccg gccaccggcg gcacccacag cgtccggccg
     2401 ccgtccagca cggtggtcac tcggtagggt tgcgcgccgt cggccaggct cacgcgcgcc
     2461 tcgaaggtgt tttccagcaa ggccatccac agcggacccc ggtcctccgg cgccgtcaac
     2521 ccgagcgcca ggatggcgct ggtgagctcg cgctgcgtct ggctgcgccg cagaatgccg
     2581 cgcccccaga tgcgccgcgt cagcagcaca tagaccaggc ccaggcccag caccgacacc
     2641 agcatggcca gctcgtcgct gagccagcgg ttcgcctgca gcagccggta gcacagcaag
     2701 atggtgacgc ccgcgcccca ggtcagcagc acctggcgcg tccagtcgtc cagctcgaac
     2761 acccgaacct cacggtgcgt gaccgcgcgc ccggccaccg ccaccaccac cagcccgtac
     2821 agccaggcct gcgacagcgg cggcgcccac cccagcagca aagccccctc gcgcaggccg
     2881 aacaggcaga acgcagccgg gatccagatc agcgcctgcg tgcgcgcctc gtcgcgcgcc
     2941 accgaatcgc cccccgaatg gagctgccac tggcgcaccg cctgtaccgt aaccaccacc
     3001 accgccgcca gcagcatggc gcggctggcc cagttgggcg acggcaacca ccagccggca
     3061 tcggccagcg cccacagcag gaacaggccg gccaccaccc agacgcgcca gccgcccatt
     3121 tcgggcaagc gactcgggta gtgcgacaga atgctcgcca cggcgagtgc ggcgaccagg
     3181 ctgccggcat gctgcagcac caccagggca tggaacaggg tcgagggcag cgccagctcc
     3241 cgcgtaccga acaccgccac gccggccagc accagcgaga tcgccatcgc ccccacggcg
     3301 ctgcgcgggg cggccggctg gggtgggtac acggtgatca cccagccggc catcagcatg
     3361 atggccgcct gcagcaccat cagggtccag aacagcggtg gcaggctgcc cggcggccgc
     3421 gtcggcgccg ggcgcagcaa caaggcctct tcctgcgcgc catgcgccac catcgtcagc
     3481 cgcaacgacg cgtcgccgag caggcgcgcc agccggtcct gccgctcata aaaccggccc
     3541 cagtcggcat aactggccaa ctcgtccggg ttggcccgca agtcatcgag ctgcagcgtc
     3601 atggcttgcg ttccatcggc ggtcgcgagt gcctgcagct gcgccccgac gtgcacgccg
     3661 gccaccgccg ccgggctgcc cggcaccacg tccacgacct gaacctgccc gtcctgcgcc
     3721 gccagccgca ggcccagcca cggctggcga gtggccagcg ccagcgccag tgtggtcagc
     3781 aacaccgccc acaggccggc cagcaggatg gcctggcggt acagccaggg cgtcgcctgg
     3841 gccggccgtt cgcgctcgag caggtccagt tgatccagcg gcgtgcgcca gctgccggac
     3901 aggccggact cggtgtccag cagccccggc agcgtgggcg gcaggtccga ttcggtgtag
     3961 agcgcgctgg gcagggtggg tgaaggatcg gacgggttca cggtcaggat gggggacaat
     4021 ccaagcgtga ttctgcggca taccttcacc ccatccaaca acacccggct ggcccacctg
     4081 tgcggcccga cggacgagca cctgcgcacc atcgaagccg gcctgcaggt gagcatcgcg
     4141 caccggcacg agcagttcag gatcgatggc gcccgcaagt cggccgagcg cgccctgacc
     4201 gtgctgcagg cgctgtacga actggccgac cgcccgatcc gcaccgagac ggtgcagctg
     4261 atgctggccg gcgacggcga gccgctggcc gaggacgcag gcggcgccca tcgcctggtc
     4321 acgcgccgcg gcgacctgaa ggcgcgcacg cccaaccaga gcgtgtacct ggagcacatc
     4381 gcccagcacg acatcacctt cggcatcggc ccggccggca ccggcaagac ctatctggcg
     4441 gtggcctgtg cggtcgacgc gctggagcgc cagtcggtgc agcgcatcgt gctgacgcgc
     4501 ccggccgtgg aggccggcga gcgcctgggc tttctgccgg gcgatctgac gcagaaggtc
     4561 gacccctacc tgcgcccgct gtacgacgcg ctgtacgacc tgatgggctt cgaccgcgtg
     4621 cacaaggcct tcgagcgcca gcagatcgaa atcgcgccgc tggccttcat gcgcgggcgc
     4681 acgctcaacc acgccttcgt catcctggac gaggcgcaga acaccacgcc cgagcagatg
     4741 aagatgttcc tgacccgcat cggcttcggc agcaaatgcg tggtgacggg cgacgtcagc
     4801 cagatcgatc tgcccaaggg cgccaccagc ggcctgatcg acgccgaacg ggtgctgcgg
     4861 cgcgtcaagg gcctggcctt cacccgcttc accagcgccg acgtggtgcg ccacccgctg
     4921 gtggcgcgca tcgtggacgc ctacgacgcc gccgggcggc ctgcccgcac cccgaaaaac
     4981 tcctgatttg atagctgctg gcgcacgtcc aataagcgcc agaggccgat ttgacccgca
     5041 taccatggcc acgccctcgc ttcgcctgtc gctgcagttc gcccgctcgc ccgagatggc
     5101 gccgcaccgg gccgcgctgc cgcgcgcgca ggtcctgcgc tggctgcgcg cggcgctggg
     5161 ggcggacgtg cggctggccg aaatcgcggt gcgggtggtc ggcgccgacg aaggccgcca
     5221 gctcaaccgc gactaccgcg gcaaggacta cgccaccaac gtgctgacct tcgactacag
     5281 cgtcgcgccg cagctggcgg ccgacctggt gctgtgcgcc ccggtggtgg cgcgcgaggc
     5341 ggccgaactg gccacgccgc tggcccagca ctacgcccac ctgctggtgc acggcgccct
     5401 gcacgcccag ggctgggacc acgaaaccag cgaacaggag gccgaagcca tggaagcgcg
     5461 cgaaaccgcc atcctggcgg cgctgggcgt gcccgacccc tacgcgcgct gacgccggtg
     5521 gccgcgggcg aaaccttgtc catcgccgcc gtcgtggcgc aaaaaaagga ctgacgccag
     5581 caaggcgtca gtcccgcgct ttggcccagg ggtgccgcca ggcaccccgg tctgcgccct
     5641 ggtggagcga aatcaaccca tgcggcgacg catcatgacc cagccgaagg ccgccatcag
     5701 gctggccagc agcatcatgg cccactcacc cagcgtggga accggggcca cgttgaccgc
     5761 gcagttttcg ctcatggcgg tgcgcaccag cggccccagt tgggtcacca cggtgccgcc
     5821 ctgggcactg gtcgcgccac tgaggctgta ggtaccgttg ttgagcgtgg ggccgatggc
     5881 ccctgccggg atctgcaccg tcatctggaa cgtgccctgc tggccgggct gcagcgtacc
     5941 gatactgcag gtcgccgggt tgcccgggct tgcgggcgcc gtacaggtgc cgccaccgaa
     6001 cgtggggttg gtgatgctga cgaacgtggt ggtcaccggc gtgctgtcgg acgtgacggg
     6061 catggacacc gaggccgtca caccgtacat cgggtccgtc ccgttgttgc ggtaggtgta
     6121 ggtgtaggtg acgttggtgg tgccgccccg gttcagacag atcgtggccg gcccggccgc
     6181 ggacacccac aggctggggc cttggacggc cgcctgggtg cggacatcgt tcacgtacag
     6241 cgcgccggca tgcccgccct ggccgcagcc ggcggccagc aagcgcagcg tcagttggtc
     6301 gcccaccgcc gccgtcaccg gaatgtcgaa cgcctgccag ttggtgtagc ggtgagagcc
     6361 ggccgaattc agcacccagg ggacacccgg ttcattggcg tagttgaacg tgtggtaaag
     6421 gctggtgccg tgctggttgg tgacctcgat gaagaaatac ggctgttcat cgggtgcgtg
     6481 tcccgggtcc tccagcaccg gcgccgcatt gaagcggata tgcaccttgc catccgcctc
     6541 gatgtcgccc gccgccaccg tcagcacctg ttcgatcgcg ctggccgtgt tgccgtcacc
     6601 gttgctgaac tcttcgtgca cacgggccac gcggttggtc caggtcaggt tgccgtcggt
     6661 ggcagccgca ttgcccgggc cgttcagcac cgccgactgg ctggtgccgg tggtcaggcc
     6721 caggtggctc cactgggtcg ggggaaacgt gggaatggca ccaggaatgg caaacccagg
     6781 tgtcgtccag ccggtgaaat cgggagactg ctcaaaaccg ccgttgacga agtcggcgtg
     6841 ggccgacgtg gtcagcagca gcagggcggc gccgacggcg gccgcagcac ccctggcgcg
     6901 ctgaatggaa cgaaaaagct tcacaatggt ttcctccggt agatgggtac ttctgaacaa
     6961 acgaatcaca cgagcctcgc cagtttatgc cgtcacccgc gagcatgtca agcaccgtgc
     7021 caccgaaaac gtcggtacac gcagcgccaa cgcggtcatt atcacacagc gttacagacg
     7081 ttcggtttgg ccctgtatgc gccccctgat gccgaacggc acgacgggca ggccatgccc
     7141 tcggccctca tccatgaccg acggccggac gcgccggcgc cacgcgcagc gggatcgtca
     7201 ccggcccgtc gttcaccagc gccacctgca tgtcggcggc aaattcgccc gtggccacct
     7261 gtgggtgtgc ggcgcgcgcc tgccgcacga agtgttcgta cagccggcgc ccatcctcgg
     7321 gcgcggcggc gccggtgaag ctgggccggt tgccgcccgc ggtgtcggcc gccagcgtga
     7381 actggctgac caccagcagg ccgccggcca cgtcctgcac gctcaggttc atcttgccgg
     7441 cggcgtcgct gaagatgcgc agcttcagca gcttggccag cagcttgtcg gccacggcct
     7501 cgtcgtcgcc acgttcggcg cacagcagca ccagcaggcc tggcccgatg gcgccgatga
     7561 cgcgccccgc cacgctgacg ctggcctggg acacacgctg gatcagggct tgcatggtgg
     7621 gttggctacc gtggtgcgtt gaacgacaaa ccatggcgcc gcggccatcg gcgcccggcg
     7681 gggtcgtgcg tcatggttcg tctcgtgaag ggcgctgcgc ctcggctggg gccgcgccca
     7741 gttcggtgga gatcggctcc acgccgtcgg gcagcatctg gatgctggcg tgcagccccg
     7801 gcaccgccgc cagcagcgcc tgctcgacct cgccgcgcaa ggcgtcggcg cgcgccaggg
     7861 tccagcccgg cggcaggtgc atgtgcaggt tgacgaagcg gcgctggccg gccttgcgcg
     7921 tcagcacgtg gtcgaacagc accgcggccg gcccgccgtg gcgcgcggcg aactgcgcca
     7981 gcacggcgtc gatgcgcgcc tgcgtgtcgg gctcgacggc ggcgtccatc aggccctgcg
     8041 acgagcgccg catcagacgc accccttcgc gggcgatgtt cagcgccacc ccgatggcca
     8101 ccagcggatc cagccacagc cagccggtgg cgcccgccag cagcaggccg gcgatgacgc
     8161 cgccagtggt ccagacgtcg gtcagcaggt ggcgcgcgtc ggcttccagc gcgatcgagc
     8221 ggtgccggcg cgaggcgcgc agcatcacca gcgccaggcc gccattgagc agcgcgctgg
     8281 ccagcgccag ccccatgccc cagcccagct gctccagcgg ctgcggccac cagaagcgcg
     8341 gcagcgcagc ccagacgatg gccagcgcgg cgccgatgat caggatgccc tcgaagccgg
     8401 aagaaaagta ctccgccttg tcgtgaccga acgggtggcc cgcatcgggt ggctgctcgg
     8461 ccaccgtcac catcaggaag ccgaagctgg cggcagccag gttgacgaag gattccagcg
     8521 cgtccgacag cagccccacc gagccggtga cccaccacgc cgcggccttc agcgcgatgg
     8581 tcagcagggc cacgccgatg gaggccgcca tcaggtggcg cggcgtcagc caggcgctca
     8641 gggtgcccat gctcaggcgc tcgcccaccg gccgcgagtt gcaggcaaaa aaggtcgctg
     8701 gcgcaatacc agcaagcgcc agcagctatc aaaacaggag ttcacgccag ggtcacgcgg
     8761 gcaaacctgc gcttgcccac ctgcaccacg tagctgccgg cggccagctt gagcgccttg
     8821 tcgctgacca cgctggcgtc gacgcgcacg ccgccgccgt cgatcaggcg gttggcctcg
     8881 ctggtggaag gcgccagccc ggcctgcttg agcagcacgc cgatgcccag cggcgcgcca
     8941 ctgagctgca cctcggggat cgcgtcgggc acgccgccgc ggctgcggtg ggcaaagtcc
     9001 tgctcggcgg cgtcggcggc ttgggcgctg tgaaagcgcg tggtgatctc gcgcgccagc
     9061 agcaccttgg cctccttggg gttgcggccg gcggcgacct cggcctggag ccgctcgatc
     9121 tcggccatgg accggaacga cagcagggtg taccagcgcc acatcagcgc gtcgctgatc
     9181 gacagcacct tggcgaacat ggtgttggcg tcttcggtga tgccgatgta gttgttcttg
     9241 gacttggaca tcttctcgac gccgtccagg ccctcgagca gcggcatggt caggatgcac
     9301 tgcggctcct gcccgtattc ctgctgcaag tggcggccca tcagcaggtt gaacttctgg
     9361 tcggtgccgc ccagctcgat gtcgctcttc agggccaccg agtcgtagcc ctgcatcagc
     9421 gggtagagga actcgtgcac gctgatcg
//
LOCUS       DAOOOG010000099         9247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_8716_length_9247_cov_28.613795, whole genome shotgun sequence.
ACCESSION   DAOOOG010000099 DAOOOG010000000
VERSION     DAOOOG010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8716_length_9247_cov_28.613795"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /gene="aspS"
                     /locus_tag="PK242_08415"
     CDS             <1..1184
                     /gene="aspS"
                     /locus_tag="PK242_08415"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOZ94081.1"
                     /translation="MVAGYDRYYQITKCFRDEDLRADRQPEFTQIDCETSFLNEEEIR
                     AIFQRMIREVFQQQLGVDLGAFPTMPYAEAMHRFGSDKPDLRVKLEFTELTDVMTDVD
                     FKVFSGPATTPGGRVVALRVPGGAQISRGEIDGYTEFVKIYGAKGLAWIKVNEVAKGR
                     DGLQSPIVKNIHDAAIAEILKRTGAQDGDILFFGADKTKIVNDSIGALRLKIGHSEFG
                     KANGLFEDRWAPLWVVDFPMFEHDEEDNRWVAVHHPFTSPKDGHEDLMDSDPGKCLAK
                     AYDMVLNGWELGGGSVRIHRADVQAKVFAALNIGPEDQRAKFGYLLDALQYGAPPHGG
                     LAFGLDRLITLMTGAESIRDVIAFPKTQRAQDLLTQAPSPVDEKQLRELHIRLRNPQP
                     A"
     gene            1419..3188
                     /locus_tag="PK242_08420"
     CDS             1419..3188
                     /locus_tag="PK242_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ94082.1"
                     /translation="MSFESLNLAPAILRAVQEQGYTEPTPIQAQAIPVVLQGHDLLAG
                     AQTGTGKTAGFTLPMLHLLNGRPARRPGAIRALVLTPTRELAAQVEESVRTYGQHLSL
                     KSTVIFGGVGMNPQIERIRAGVDILVATPGRLLDLEGQDHIDLSGVEILVLDEADRML
                     DMGFIHDVKKVLALLPADKQSLLFSATFSDDIRELAAGLLRNPRSIQVTPPNTTVERI
                     RQAIYPVGRNRKKDVLAHLIGEGQWSQVLVFTRTKFGANKVADYLNERGIKAMALHGN
                     KSQSARTQALADFKSGELRALVATDIAARGIDIDELPHVVNYDIPNVPEDYVHRIGRT
                     GRAGASGEAVSLVCMDEEGFMHEIERFTRQQIPVRILDGFGPEEGERAEPIAMGRQTL
                     WGGLGKPPSREVMQAAARAARQEMMQRIRDNKAAQGQRGRAAAPGRPAPARAASDGPL
                     RADDAAQGDSDAAQGRAPQGAAATGERTGRSRRRRGRGGAGGVQGQRPGSPAGAQAPR
                     APQARATREFGFDDEDDRDLEHLPRHIDPLQTNLHGRRSAPRGPSHGAVGQPDPMRTS
                     IDLMGRGKGGGGRNKSRKGGFGR"
     gene            3228..4235
                     /locus_tag="PK242_08425"
     CDS             3228..4235
                     /locus_tag="PK242_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOZ94083.1"
                     /translation="MTAPRRPRLVLLGTGGTIAGAGTAAEAGLSAAYRSAVVGAEALV
                     AGVPALARLADLRAEQLLQIDSADFTDALLLRLARRVAELCARDDVDGVVITHGTDTL
                     EESAYFLHLTVRSAKPIVLTGAMRPATALAADGPANLLHAVAVAAHPSSAGRGVLVVM
                     NEEIHSARDVSKVHAARLDAFASPHGPLGLVAQGAPRWYRALTRPHTTGSAFDIGALD
                     ALPLVGVVASHGNMRAEIYDAWLALGARAIVHAGFGGGTVPEYLKAPLASLRARGVLL
                     VRASRAAAGVLVRNANADDDAHGWVVTDDQNAPRARLLTALALTHTQDPLAVQQIFDC
                     Y"
     gene            complement(4281..5657)
                     /locus_tag="PK242_08430"
     CDS             complement(4281..5657)
                     /locus_tag="PK242_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOZ94084.1"
                     /translation="MIRPLRQTLVLWLLSLRDLLVSAGPVLLLALGVLVAAYAWLDPQ
                     PPRQVTLATGPAGSAYAEFGTRYAQALARQGIRVRLLATEGAQANLQALRDGVADLAF
                     VRGGSADPQADSEAGIASLGALFYEPVWVFHRTDATAGRAPAATPSVAKEAPGMTRLT
                     QLRGLRVNIDQPGSGVPELVQRLLAANAIGGDDIVASQLPPEAATEALLGGSLDALVL
                     VSAPESPVVGRLLRAPRIALMDVARADAYARRFAFVQSVTLPRGVADLAADLPPHDVA
                     LLATTTSLLTREQTHPALRQLFAQAAQGLHGDTGWFNRARDFPNTRTSELPVSAEGDR
                     AINGTPPFWQRYLPFWASNLLERMWLVIGGLIVLLLPLSRVVPPLYTYRVRQRVFRWY
                     ARLRRIEARLAEAPAERELLLNELDELDRLANQTTVPLAHTDELYALRGHIEAVRRRA
                     LARPYNDA"
     gene            complement(5654..6703)
                     /locus_tag="PK242_08435"
     CDS             complement(5654..6703)
                     /locus_tag="PK242_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOZ94085.1"
                     /translation="MTAVPALPYAGTLTDVQGIEVGHFTDPRRPTGCSVVLARAGAVG
                     GVDVRGAAPGTRETDLLDPANLVGVVHAVLLAGGSAWGLDAAGGVMRWLEEQGIGLPV
                     GGAPGQIVPIVPAAVLFDLHVGDARIRPDAAAGHAACQAAGSAPPAQGNVGAGAGATV
                     GKLFGAAHAMKGGIGSAAVSVDGVTVGALIAVNALGDVVDPASGRPIAGARSADGRLH
                     GSVARALAGERPLSLLSGTQTTIGVIATDAPLTKAQCQRLAGAGHDGLARAIRPVHTM
                     SDGDTLFALATGQTRALDFNVLCSMAGEAVARACVNAVQAARSLSVAGVQLPAAIDIE
                     AAGASLERAGGQVQP"
     gene            complement(6700..7158)
                     /locus_tag="PK242_08440"
     CDS             complement(6700..7158)
                     /locus_tag="PK242_08440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOZ94086.1"
                     /translation="MPEFVDTSAPGECIFCKLVAGQIPSQRVHEDALTLAFMDLGQVN
                     PGHVLVATRRHAATLLDITAEEAAAVMRTARRVARAAQAAFAPDGITLLQASGAVAGQ
                     TVGHFHLHVLPRHTGDGVDLAWPRKEPGPAALADYAERLRAALAREEAHP"
     gene            7530..8669
                     /locus_tag="PK242_08445"
     CDS             7530..8669
                     /locus_tag="PK242_08445"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOZ94087.1"
                     /translation="MSVAQFFMPSSNLLGAGALAEAVKQMGGLGFRKALIVTDEPLVK
                     LGLASQLAGLLQAQGIASAVFDQVRPNPRVADVNAGLAALRQLDADCVVSLGGGSPHD
                     CAKGIALLATNGGRIEDYEGLDRSARPQLPLIAVNTTAGTASEMTRFTIITDETRHVK
                     MAIVDKHVTPILSVNDPQLMTGMPASLTAATGMDALTHAVEAYVSTAANPITDACALQ
                     AVALIARHLPTAVQQPQDATAREQMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYDLP
                     HGVCNALLLPHVERFNLRAAAARLDDIGRTLGAHNAELAGLDPIAAIQKLAQRVGIPP
                     NLRALGVKPEDFETLAGNAMKDVCGLTNPVQPTLHDVIGIFQAAW"
     gene            complement(8719..9210)
                     /locus_tag="PK242_08450"
     CDS             complement(8719..9210)
                     /locus_tag="PK242_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ94088.1"
                     /translation="MSAAGVLVRRVDYRAAVDRSALVELLNAYAIDPMGGAEPLADEV
                     RQRLCDDLAAHPLAASFIAWRGDEPLGLANTFLGYSTFKARPLLNVHDIAVRADWRGR
                     GVGQALLAACEQHARQKRCCKLTLEVLSGNARALRSYQRFGFAPYVLDPREGQALLMQ
                     KWL"
BASE COUNT         1400 a         3330 c         3273 g         1244 t
ORIGIN      
        1 tgatggtggc cggctacgac cgctactacc agatcaccaa gtgcttccgc gacgaggacc
       61 tgcgcgccga ccgccagccc gagttcacgc agatcgactg cgagacctcg ttcctgaacg
      121 aggaggaaat ccgcgccatc ttccagcgca tgatccgcga ggtgttccag cagcagctgg
      181 gcgtggatct gggcgcgttc cccaccatgc cttacgccga ggcgatgcac cgcttcggtt
      241 cggacaagcc cgatctgcgc gtcaagctcg aattcaccga gctgaccgac gtgatgaccg
      301 acgtggactt caaggtcttc agcggccccg ccaccacccc gggcggtcgc gtggtcgccc
      361 tgcgcgtgcc cggtggcgcg cagatcagcc gcggcgagat cgacggctac accgaattcg
      421 tcaagatcta cggcgccaag ggcctggcct ggatcaaggt caacgaagtg gccaaggggc
      481 gcgacggttt gcagtcgccc atcgtgaaga acatccacga tgccgccatc gctgaaattc
      541 tcaagcgcac cggtgcgcag gacggcgaca tcctgttctt cggcgccgac aagacgaaga
      601 tcgtcaacga cagcatcggc gcgctgcgcc tgaagatcgg ccacagcgag tttggcaagg
      661 ccaacggcct gttcgaagac cgctgggccc cgctgtgggt ggtggacttc cccatgttcg
      721 agcatgacga ggaagacaac cgctgggtgg ccgtgcacca ccccttcacc agcccgaagg
      781 acggccacga agacctcatg gacagcgacc ctggcaagtg cctcgccaag gcctatgaca
      841 tggtgctgaa cggttgggaa ctgggcggcg gctcggtgcg tatccaccgc gccgacgtgc
      901 aggccaaggt gttcgccgcg ctcaacatcg gccccgagga ccagcgtgcc aagttcggct
      961 acctgctcga cgccctgcag tacggcgcgc ccccgcacgg cggcctggcc ttcggtctgg
     1021 accgcctgat caccctgatg accggcgccg agtcgatccg cgacgtgatt gccttcccca
     1081 agactcagcg cgcgcaggac ctgctgaccc aggcgcccag cccggtcgac gagaagcagc
     1141 tgcgcgagct gcacatccgg ctgcgcaatc cgcagccggc ctgacgcgcg ccggcgtcgc
     1201 cggcgggcca cagcgccccg gcgaccgcgg catgcgataa tgcgcgggcc tgcggatcac
     1261 cagttcgccg catggtcagc tcttccgctg gggtcttccg ccacgatgcc gggcggccca
     1321 cttcaagaag aacccgctgc cgcccctgac tggccggctt gtcagccgga gcgggcagtc
     1381 agcaccagcg ccgaccagag accggcgcaa ccaatctgat gagctttgaa tcactgaacc
     1441 tggcgcccgc catcctgcgc gcggtccaag aacagggcta caccgagccc acccccatcc
     1501 aggcccaagc cattcccgtg gtgctgcagg ggcacgacct gctggccggc gcccagaccg
     1561 gcaccggtaa gacggccggc ttcacgctgc cgatgctgca cctgctgaac ggccggccgg
     1621 cgcgtcgccc cggcgccatc cgcgcgctgg tgctcacgcc cacgcgcgag ctggcggcgc
     1681 aggtcgaaga atccgtgcgc acctacggcc agcacctgag cctgaagtcg accgtgatct
     1741 tcggcggcgt cggcatgaac ccgcagatcg aacgcatccg cgccggtgtc gacatcctgg
     1801 tggccacccc cggccgcctg ctggacctgg agggccagga ccacatcgac ctgtcgggcg
     1861 tcgaaatcct ggtgctggac gaagccgacc gcatgctcga catgggcttc atccacgacg
     1921 tcaagaaggt gctggcgctg ctgccggccg acaagcagag cctgctgttt tccgccacct
     1981 tcagcgacga tatccgcgag ctggccgccg gcctgctgcg caacccgcgc agcatccagg
     2041 tgacgccgcc caacaccacg gtggagcgca tccgccaggc catctacccg gtgggccgca
     2101 accgcaagaa ggacgtgctg gcgcacctga tcggcgaggg ccagtggagc caggtgctgg
     2161 tgttcacgcg caccaaattc ggcgccaaca aggtggccga ctacctgaac gagcgcggca
     2221 tcaaggccat ggcgctgcac ggcaacaaga gccagagcgc gcgcacccag gcgctggccg
     2281 acttcaagag cggtgagctg cgtgccctgg tggccaccga catcgccgcg cgcggcatcg
     2341 acatcgacga gctgccgcat gtggtgaact acgacatccc caacgtgccc gaagactacg
     2401 tgcaccgcat cggccgcacc ggccgcgccg gcgccagtgg cgaggcggtc agcctggtgt
     2461 gcatggacga ggaagggttc atgcacgaga tcgagcgttt cacgcgccag cagatcccgg
     2521 tgcgcatcct cgacggtttt ggccccgagg agggcgaaag ggccgagccg atcgccatgg
     2581 gtcgccagac cctgtggggc ggtctgggca agccgcccag ccgtgaggtg atgcaggccg
     2641 cggcgcgcgc cgcgcgtcag gagatgatgc agcgcatccg cgacaacaag gccgcccagg
     2701 gccagcgtgg gcgggcggcg gcgcccggcc ggccggcgcc ggcacgcgcg gcgtcggatg
     2761 gcccgttgcg ggccgacgat gcggcgcagg gcgattccga cgccgcccag gggcgcgccc
     2821 cgcagggtgc tgcggcgacg ggcgaacgca cgggccgcag ccgccggcgg cgcggtcggg
     2881 gcggcgctgg gggcgtgcag ggccagcgcc ccggcagccc tgcgggcgcc caggcgccgc
     2941 gcgcaccgca ggcgcgagcc acgcgcgagt tcgggttcga cgacgaggat gaccgcgacc
     3001 tcgaacacct gccgcgccac atcgacccgc tgcagaccaa cctgcacggc cgccgcagcg
     3061 cgccgcgcgg ccccagccac ggcgccgtcg gccagcccga cccgatgcgc accagcatcg
     3121 acctgatggg gcgcggcaag ggcggcggtg gccgcaacaa atcgcgcaag ggcggcttcg
     3181 gccgctgagc aacggatgcc agaccgacgg ccgcggccct gcctgccatg accgcccccc
     3241 ggcgcccgcg ccttgtcctg ctgggcaccg gcggcaccat tgccggcgcc ggcacggcag
     3301 cggaagccgg gttgtcggcc gcctaccgtt cggccgtggt cggcgccgag gcgctggtgg
     3361 ccggcgtgcc ggcactggcg cggctggccg atctgcgtgc cgagcagctg ttgcagatcg
     3421 attcggccga cttcaccgac gcactgctgc tgcgtctggc gcgccgcgtc gccgagctgt
     3481 gcgcgcgcga cgacgtcgat ggcgtggtca tcacccacgg caccgacacg ctggaagagt
     3541 cggcctattt cctgcatctg accgtcagga gcgccaagcc catcgtgctg accggggcca
     3601 tgcggccggc caccgcgctg gcggcggacg ggccggccaa cctgctgcac gcggtggccg
     3661 tggcggcgca cccgtcttcc gccgggcgcg gcgtgctggt ggtgatgaat gaagaaatcc
     3721 acagcgcgcg cgacgtcagc aaggtgcatg cggcgcggct ggacgccttt gcctcgccgc
     3781 acggtccgct gggcctggtg gcgcagggcg cgccgcgctg gtaccgcgcg ctgacgcgcc
     3841 cgcacaccac gggcagcgcg ttcgacatcg gcgcgctcga tgcgctgccg ctggtcggcg
     3901 tggtggccag ccacggcaac atgcgggccg aaatctacga tgcctggctg gcgctgggcg
     3961 cgcgcgccat cgtgcacgcg ggcttcggcg gcggcaccgt gccggagtat ctgaaggcgc
     4021 cgctggccag cctgcgcgcg cgcggcgtgc tgctggtgcg cgcctcgcgc gccgcggcgg
     4081 gggttctggt gcgcaacgcc aacgccgacg acgatgccca cggctgggtc gtcaccgacg
     4141 accagaacgc gccgcgcgcg cgcctgctga cggcgctggc gctcacgcac acgcaggacc
     4201 cgctggccgt tcagcagatc ttcgattgct attgaaatgg tagcttctgg cgcttgatgg
     4261 ataagcgctg gaggccgatt tcatgcatcg ttgtacggtc tcgccagcgc ccgtcggcgc
     4321 accgcctcga tgtggccgcg cagcgcgtac agctcgtcgg tgtgggccag tggcaccgtg
     4381 gtctggttgg ccaggcggtc aagttcgtcc agctcgttca gcagcagttc gcgctcggcc
     4441 ggcgcttcgg cgagccgcgc ctcgatgcgg cgcagccgcg cataccagcg gaacacgcgc
     4501 tggcgcaccc ggtaggtgta gagcggcggc accacgcgcg acaagggcag cagcagcacg
     4561 atcaggccgc cgatcaccag ccacatgcgc tccagcaggt tgctggccca gaacggcagg
     4621 tagcgctgcc agaacggcgg cgtgccgttg atggcgcggt cgccctcggc gctgacgggc
     4681 agctcgctgg tgcgcgtgtt gggaaagtcg cgcgcacggt tgaaccagcc ggtgtcgcca
     4741 tgcaggccct gcgccgcctg cgcgaacaac tggcgcagcg ccgggtgggt ctgctcgcgc
     4801 gtcagcagcg aggtggtggt ggccagcagc gccacatcgt ggggcggcag gtcggccgcc
     4861 agatcggcca cgccgcgcgg cagcgtgacc gactgcacga acgcgaagcg gcgcgcatag
     4921 gcgtcggccc gggccacgtc catcagcgcg atgcggggcg cgcgcagcag gcggccgacc
     4981 accggcgact cgggcgcgct gaccagcacc agcgcgtcca gggagccacc cagcagggct
     5041 tcggtggcgg cctcgggcgg cagctggctg gccacgatgt cgtcgccgcc tatggcgttg
     5101 gccgccagca ggcgctgcac cagttcgggc acgccgctgc cgggctggtc gatgttcacg
     5161 cgcaggccgc gcagctgcgt cagccgggtc atgccgggcg cctcttttgc tacagaagga
     5221 gtagctgctg gcgcacgacc ggcggtcgca tcagtccgat ggaacaccca caccggttca
     5281 tagaacaggg cgcccagcga ggcgatgccg gcttcgctgt cggcttgcgg gtcggcgctg
     5341 ccgccacgca cgaaggcgag gtcggccacg ccatcgcgca gcgcctgcag gttggcctgc
     5401 gcaccttcgg tggccagcag gcgcacgcgg atgccctggc gcgccagcgc ctgcgcatag
     5461 cgcgtgccga actcggcgta ggcgctgccg gccggcccgg tggccagggt cacctggcgt
     5521 ggcggctgcg ggtcgagcca ggcgtaggcc gccaccagca cgcccagcgc cagcagcagc
     5581 accggcccgg ccgacaccag caggtcgcgc agcgacagca gccacagcac gagggtctgg
     5641 cgcagcgggc ggatcatggc tgaacttggc ccccagccct ttccaggctt gcgccggcag
     5701 cttcgatatc gatagcagcc gggagttgca cgccggccac gctcaggctc cgcgcggcct
     5761 gcacggcgtt cacgcaggcg cgtgccaccg cctcgccggc catgctgcac aggacgttga
     5821 agtccagcgc ccgcgtctgc ccggtggcga gggcgaacag cgtgtcgccg tcgctcatgg
     5881 tgtgcaccgg gcggatggca cgcgccagcc cgtcgtggcc ggcgccggcc aggcgctggc
     5941 actgcgcctt ggtgagcggc gcgtcggtgg cgatcacgcc gatggtggtc tgcgtgcccg
     6001 acagcaggga cagcggccgt tcgccggcca gcgcccgcgc cacgctgccg tgcaggcgcc
     6061 cgtcggcact gcgggcgccg gcgatgggcc ggccgctggc cggatcgacc acgtcgccca
     6121 gcgcgttgac ggcgatcagc gcgcccacgg tcacgccgtc cacgctgacg gcggcgctgc
     6181 cgatgccgcc cttcatggcg tgcgcggcgc cgaacagctt tcccacggtg gcgccggcgc
     6241 ccgcgcccac gttgccctgc gccgggggcg cgctgccggc ggcctggcag gccgcatgac
     6301 cggcggcggc gtcggggcgg atgcgcgcgt cgcccacgtg caggtcgaac agcaccgccg
     6361 ccggcacgat gggcacgatc tgccccggcg cgccgcccac cggcaggccg atgccctgct
     6421 cctccagcca gcgcatcacg ccgccggcgg cgtccagccc ccaggcgctg ccgccggcga
     6481 gcagcacggc atgtaccacg ccgaccaggt tggcggggtc gagcaggtcg gtttcgcgcg
     6541 tgcccggcgc ggcgccgcgc acgtccactc cgcccacggc gccggcgcgc gccagcacca
     6601 cgctgcagcc ggtggggcgg cgcgggtcgg tgaagtggcc gacctcgatg ccttgcacgt
     6661 cggtcagcgt gcccgcgtag ggcagtgcgg gcacggcggt cacggatgtg cctcctcgcg
     6721 ggccagcgcg gcgcgcagcc gttcggcgta atcggccagc gcggccgggc cgggttcctt
     6781 gcgcggccag gccagatcga cgccatcgcc tgtatggcgc ggcagcacgt gcaggtggaa
     6841 atgccccacc gtctgccccg ccaccgcgcc gctggcctgc agcagcgtga tgccgtccgg
     6901 cgcaaaggcc gcctgcgccg cgcgcgccac gcgccgcgcg gtccgcatga cggcggcggc
     6961 ctcctcggcc gtgatgtcca gcagcgtggc ggcgtggcgc cgtgtggcca ccagcacatg
     7021 cccggggttg acttggccca ggtccatgaa ggccagggtc agcgcatcct cgtgcacgcg
     7081 ctgggacgga atctgaccgg ccaccagttt gcagaaaatg cattcaccgg gcgcggaagt
     7141 gtcgacgaat tcgggcatga cggtgctttc agggtcggca gttggacacc ccacgattat
     7201 tgattcggcc tttggatgtc ctgaacttca ggaatccgaa gccatgaacc cgccgcgcag
     7261 cgcccgcggt gtggggtgga tgcgggcatc cggcttacct gtcgcttgac cgggctcaag
     7321 gcggcgccgc acgctgcggg caacaatgct tgaagctgga aacggcattg accgctggtc
     7381 atctgcaggg cgggagtcat gcaggcaggt gcttgcggct gtgatcgcat tgacccgttc
     7441 ggtgggtgcg cccgatcgcc gattccgggt tcagcgtgcc cccaccgacg gacgcggtgg
     7501 ggatggggtt ccacacgtca ggagttgaca tgagcgttgc acagttcttc atgccatcca
     7561 gcaacctgct gggcgcaggc gccctggccg aggcggtcaa gcagatgggc ggcctcggct
     7621 ttcgcaaggc cctgatcgtc accgacgagc cgctggtcaa gctggggctg gccagccagc
     7681 tggccggcct gctgcaggcg cagggcatcg ccagcgccgt gttcgaccag gtcaggccca
     7741 acccgcgcgt ggccgacgtc aacgccggcc tggccgcgct gcgccagctg gacgccgact
     7801 gcgtggtgtc gctcggcggc ggttcgccgc acgactgcgc caagggcatt gcgctgctgg
     7861 ccaccaacgg cgggcgcatc gaggactacg aaggcctgga ccggtcggcc cggccgcagt
     7921 tgccgctgat cgccgtcaac accacggccg gcacggcgtc ggagatgaca cgcttcacca
     7981 tcatcaccga cgagacgcgg cacgtgaaga tggccatcgt cgacaagcat gtgacgccga
     8041 tcctgtcggt caacgacccg cagctgatga ccggcatgcc cgccagcctg accgccgcca
     8101 ccggcatgga cgcgctgacg cacgcggtcg aggcctatgt gtcgaccgcc gccaacccga
     8161 tcaccgacgc ctgcgcgctg caggcggtgg cgctgatcgc gcgccacttg cccaccgcgg
     8221 tgcagcagcc ccaggacgcc acggcgcgcg agcagatggc gtacgcgcag tttctggccg
     8281 gcatggcgtt caacaacgcc tcgctgggct acgtgcatgc catggcgcac cagctgggcg
     8341 gcttctacga cctgccgcac ggcgtgtgca acgccctgct gctgccgcat gtcgagcgct
     8401 tcaacctgcg ggcggcggcg gcgcgcctgg acgacatcgg ccgcacgctc ggcgcgcaca
     8461 acgccgaact ggccggactc gatccgatcg ccgcgatcca gaagctggcg cagcgcgtgg
     8521 gcatcccgcc caacctgcgc gcgctgggcg tcaagccgga ggacttcgag actctggctg
     8581 gcaacgccat gaaggacgtc tgcggcctga ccaacccggt gcagcccacg ctgcacgacg
     8641 tgatcggcat cttccaggcc gcctggtgac tgcccgcgcg tggccggcgc tgtcaggcgc
     8701 ggccgcgccc ggcatggctc acagccattt ctgcatcagc agcgcctgac cttcgcgcgg
     8761 atccagcacg tagggcgcaa aaccgaagcg ctggtagctg cgcagggcgc gtgcgttgcc
     8821 gctcagcacc tccagcgtga gcttgcagca gcgcttctgg cgcgcatgct gttcgcacgc
     8881 cgccagcagc gcctgaccga cgccgcgccc gcgccagtcg gcgcgcaccg cgatgtcgtg
     8941 cacgttcagc agcgggcggg ccttgaaggt cgaatagccc aggaaggtgt tggccaggcc
     9001 cagcggctcg tcgccgcgcc aggcgatgaa gctggccgcc agcgggtgcg ccgccaggtc
     9061 gtcgcacagg cgctggcgca cctcgtcggc cagcggctca gcgccgccca tggggtcgat
     9121 cgcgtaggcg ttgagcagct cgaccagggc gctgcggtcc acggcggcgc ggtagtcgac
     9181 gcgccgcacc agcacgccgg cagcgctcaa gccagatcct cgaccggaat gcaactgcaa
     9241 aacaggt
//
LOCUS       DAOOOG010000100         9118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_8896_length_9118_cov_19.450623, whole genome shotgun sequence.
ACCESSION   DAOOOG010000100 DAOOOG010000000
VERSION     DAOOOG010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8896_length_9118_cov_19.450623"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1003)
                     /locus_tag="PK242_08455"
     CDS             complement(20..1003)
                     /locus_tag="PK242_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOZ94089.1"
                     /translation="MVFVDLADSTAAYAALGNAPVAEVVSKVTQWIGRVCDAHQGRIV
                     KYLGDGVLAQFADAQQAIEAVVFMQQSHTERLRKWPEPLHMGLKIGMACGPVVHKDGD
                     TYGDPVNLAARLSDLAGAHTIWADDTVITHLRQTHPRNSPVVDKVRYRSLGLIRVRGL
                     AQPQSVFQIEWNEELSSDLMTVRGALHDLSRDAQSSGASIALAWLGTSRVFSVSDMPV
                     TIGRTPDSSFVVSDQRVSRTHARIEFINGSFMLSDVSSFGSWVRFADKPEAEILLRRN
                     QCILHAAGEIALGAPFSDFSAATVAFDVAAGQLAPLGRAATRSLFGDSTQS"
     gene            1274..1618
                     /locus_tag="PK242_08460"
     CDS             1274..1618
                     /locus_tag="PK242_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94090.1"
                     /translation="MRWFKHGGLTALYERLSGSTPDPERMAYRVEDIRVAMLDMLGEQ
                     GEHSHPQVARRIRYAGDALALWYARADLMAALAGLHGEQLARTRMVSLSVLFEGTLPK
                     GMASRPSSLSRF"
     gene            complement(1687..2643)
                     /locus_tag="PK242_08465"
     CDS             complement(1687..2643)
                     /locus_tag="PK242_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOZ94091.1"
                     /translation="MAHIYVYSPSGAVRDRAAFRLGVRRLQAQGHEVEIDPTALASWQ
                     RFAGDDATRLAAIGRAAASGADVALMTRGGYGLSRLLPSLPYRQLARAIAKGTRFVGF
                     SDFTALQLALLGRTGAVTWAGPALCEGFGGAGAPGSAKGERAAPDDIMQACFDDLLSG
                     AGEGSGWRLPSSDVLALNGRQGALAQGAPLWGGNLTVLLSLLGTPWWPAVRHGVLFLE
                     DVGEHPYRIERMLAQLLHAGVLAQQKLIVLGQFTDYSLSAHDRGYSMARVVEWLRANL
                     KVPVLTGLPFGHVPTKVLLPVGQPVQALIEGRDALLLWGHLH"
     gene            complement(2708..4114)
                     /gene="tadA"
                     /locus_tag="PK242_08470"
     CDS             complement(2708..4114)
                     /gene="tadA"
                     /locus_tag="PK242_08470"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOZ94092.1"
                     /translation="MRVGEDRLTDDQAMRLALQQAQAAAALGEVPVGAVVVRDGRVIA
                     SGHNAPIGAHDPTAHAEVVALRAAAQALGNYRLDGCTLYVTLEPCAMCAGAMLHARLD
                     RVVFGASDPKAGAAGSVLDLFAETRLNHQTEVRGGVLAHECAALLRQFFKPRRINRDP
                     LRDDALRTPDTAFQDLPGHPWPPHYVSDLPALAGLRLHWVDEGPPDAPLTWLCLHGNP
                     GWSYLYRHMMAAFAAAGHRVVTPDLIGFGRSDKPKKEGTHRFDWHRQVLLEFIERLDL
                     QRIVLVVQDWGGLLGLTLPMVAPGRYLGLLAMNTMLATGDAPLPAGFIDWRQWCAERP
                     AYDIARLLARAEPSLSAAECAAYAAPFPDAGHRAATRAFPRLVPAHADQDGAAISRQA
                     RDFWRHQWRGHSLLVVGARDPVLGQPVMAELAALIRGCPPPWVLPEAGHFVSEHGRAI
                     AERALQHFGAARGSTAAL"
     gene            complement(4173..4853)
                     /locus_tag="PK242_08475"
     CDS             complement(4173..4853)
                     /locus_tag="PK242_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HOZ94093.1"
                     /translation="MYLPSQFATQDQRLAARVMREHPLASLITTDDAGLPYVSHLPLH
                     LQHEDTGFRLLGHMARPNVQWRHLQQRARALVTFMGPHAYMSPSVYPDRARVPSWNYV
                     AVHCSVTARLLDHDDAHAKDALLKCLIGDHEPAYAAQWRDMEGELARKLLAGIVAFEL
                     QVDHWQCKIKLNQHRPESHAALHAAYLGGGAQQQALADWMVRLGLVGGSSAPSGGPDD
                     PCGSNADS"
     gene            complement(5026..6642)
                     /gene="guaA"
                     /locus_tag="PK242_08480"
     CDS             complement(5026..6642)
                     /gene="guaA"
                     /locus_tag="PK242_08480"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOZ94094.1"
                     /translation="MPHDKILILDFGSQVTQLIARRVREAHVYCEVHPCDVSSDWVRE
                     HAADGRLKGVILSGSHASVYEVDDKAPDAVFELGIPVLGICYGMQTMAQQLGGKVEGG
                     HTREFGYAEVRAHGHTPLLKDMADFTTPEGHGMLKVWMSHGDKVTALPPGFKLMASTP
                     SCPIAGMADEARGFYALQFHPEVTHTVQGRAMLERFVLQICGARPDWVMRDHIEEAVQ
                     AIRTQVGDEEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLHEGDMVMAMF
                     ADRLHARVVRVDASELFLNALRGVSDPEHKRKIIGRLFVDVFKAEAEKLKAGGAGHKG
                     ASFLAQGTIYPDVIESGGGKGKKAVVIKSHHNVGGLPEQLGLKLLEPLRDLFKDEVRE
                     LGLALGLPAEMVHRHPFPGPGLGVRILGEVKQEYADLLRHADAIFIEELRSTIEPHSG
                     KSWYDLTSQAFGVFLPVKSVGVMGDGRTYDHVVALRAVQTSDFMTADWAELPYELLKR
                     TSSRIINEVRGINRVTYDISSKPPATIEWE"
     gene            complement(6743..8212)
                     /gene="guaB"
                     /locus_tag="PK242_08485"
     CDS             complement(6743..8212)
                     /gene="guaB"
                     /locus_tag="PK242_08485"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOZ94095.1"
                     /translation="MRLIGQALTFDDVLLVPAYSQVLPKDVNLSTQFTRRIRLNLPLV
                     SAAMDTVTEARLAIAMAQEGGIGIVHKNLTAHEQATQVAKVKRYESGVLRDPVVITPE
                     HTVLQVMQLSDELDISGFPVIDHGRVVGIVTGRDLRFETRYDVPVREIMTPRNKLITV
                     PEGTTLDVAKALLNKHRLERLLVVDEQDRLKGLITVKDITKQTTFPNAARDAHGRLRV
                     GAAVGVGEGTEERVEALVKAGVDALVVDTAHGHSQGVIERVRWVKNKYPQVDVIGGNI
                     ATGKAARALVDAGADGVKVGIGPGSICTTRIVAGVGVPQITAIHDVSQALEGTGVPMI
                     ADGGVRYSGDIAKAIAAGAHSVMMGGMFAGTEEAPGEIVLFQGRSYKSYRGMGSIGAM
                     QQGSADRYFQESSTGNPNADKLVPEGIEGRVPYKGSVVAIMFQQAGGLRAAMGYCGCA
                     TIDDMHARAEFVQITAAGIRESHVHDVQITKEAPNYRAD"
     gene            8406..8777
                     /locus_tag="PK242_08490"
     CDS             8406..8777
                     /locus_tag="PK242_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94096.1"
                     /translation="MTLTSHTPSRRALLAGCLAGATALAGCMVAPVPGAVYTDRDGVV
                     TSTTVYAPVAPPAPYYEVQPALPYPGAVWIGGYWRWGGHRHVWVPGRYERPRPGHHYV
                     PHRWHRSPHGGWYLQGGAWMR"
BASE COUNT         1488 a         3112 c         3003 g         1515 t
ORIGIN      
        1 aatcaaagcg cagggttgct cagctctgcg tcgaatcgcc gaacagcgat cgggtcgccg
       61 ccctgccgag cggcgccagc tgacctgcgg cgacgtcgaa cgccacggtg gcggcgctga
      121 aatcgctgaa cggcgcgccc aaggcgatct cgccggcggc atgcaggatg cactggttgc
      181 gacgcagcag aatctcagct tctggcttgt cggcgaagcg aacccaggag ccaaagctgc
      241 tgacgtccga cagcatgaaa ctgccgttga tgaactcgat gcgcgcgtgc gtgcgcgata
      301 cccgctgatc gctgacgacg aaactgctgt cgggagtccg cccgatggtg accggcatgt
      361 cggacaccga aaacaccctg gacgtgccca gccaagccaa cgcaatgctg gcgccgctgg
      421 actgcgcatc ccgcgaaaga tcgtgcaacg ccccgcgcac cgtcatcaga tcggacgaca
      481 gctcttcgtt ccattcgatc tgaaacaccg actggggctg ggccagaccg cgcacgcgta
      541 tcaacccgag cgaacggtag cgcaccttgt cgacgacggg cgagttccgg ggatgcgtct
      601 gtcgcaggtg tgtgatcacc gtgtcgtcgg cccagatcgt gtgcgcccct gccagatcgg
      661 acaggcgggc agccaggttc accgggtcac cgtaggtgtc tccatccttg tgcacgaccg
      721 ggccgcaggc catgccgatc ttcagaccca tgtgcagcgg ctcgggccac ttgcgcaggc
      781 gttcggtgtg gctttgctgc atgaaaacca ccgcctcgat tgcctgttgc gcgtcggcaa
      841 attgcgccag cacaccatcg cccagatact tgacgatgcg cccctggtgg gcatcacaga
      901 cgcgcccgat ccactgcgtg accttggaga caacttcggc gaccggggca ttgcccagcg
      961 cggcatacgc agccgtgctg tcggccagat cgacgaaaac catggtgact ggagcgctca
     1021 tagcaatagg ttggctgcct cggtgatcgg gcgccctgct cctccgtgtt taccctgatc
     1081 acagcatgcc gtgcattgta aaaatggtag cacgtgagcg tcgcacgccc gacgcacgtg
     1141 gcaggcgaac cgatttgggc gttgtatttt tttctctttt cgggcctggt caacaacgcc
     1201 aatggcgcag ttggggaatt tgcactagta tggttaccaa cagttatccg taattcctat
     1261 tggtaacaag ccgatgagat ggttcaagca cggcgggctc actgccctgt acgagcgctt
     1321 gtccggctcc acgcccgacc ctgagcgcat ggcctatcga gtggaagaca ttcgagtggc
     1381 gatgctggat atgctgggtg aacagggtga gcacagccac cctcaggtgg cccgccgcat
     1441 ccgctacgcg ggcgacgcgc tggccctctg gtatgcgcgt gctgacctga tggcggccct
     1501 ggccggtctg cacggcgagc agctggcgcg cacccgcatg gtcagtctgt cggtcctgtt
     1561 cgaaggcacc ctgcccaagg gcatggcatc gcgcccttct tctctgtcgc gcttctgata
     1621 cgcccaccgt cgcgcgaacg gccagcccgc tgcctctgcc atgggccggc ggatgctggc
     1681 tggccctcag tgcaagtgcc cccacagcag cagcgcatcc ctgccctcga tcagcgcctg
     1741 caccggctgg cccaccggca acagcacctt ggtgggcaca tgaccgaaag gcaagccggt
     1801 cagcaccggc accttcaggt tggcgcgcag ccactccacc accctggcca tcgagtagcc
     1861 gcgatcgtgc gcgctcaggc tgtagtcggt gaactggccc agcacgatca gcttctgctg
     1921 cgccagcacc cccgcgtgca gcagctgcgc cagcatgcgc tcgatgcgat acgggtgttc
     1981 acccacgtct tccaggaaca gcacgccgtg cctgaccgcc ggccaccacg gcgtgcccag
     2041 cagcgacagc agcaccgtca ggttgccacc ccacagcggc gcgccctgcg ccagcgcgcc
     2101 ttgccggccg ttgagggcca ggacatccga ggaaggcagg cgccagccac tgccctcgcc
     2161 tgcacccgac agcagatcgt cgaagcaggc ctgcatgatg tcgtccggcg ccgcccgctc
     2221 gcctttggcc gagcccggcg cgccggcgcc gccgaagcct tcgcacagcg ccgggcccgc
     2281 ccaggtcacg gcgccggtgc ggcccagcag cgccagctgc agtgcggtga agtcgctgaa
     2341 tcccacgaag cgcgtgccct tggcgatggc ccgggcgagc tggcggtacg gcaaagacgg
     2401 cagcaggcgg ctcaggccgt agccaccgcg cgtcatcagc gccacgtcgg cgccgctggc
     2461 cgccgcacgg ccgatggcgg ccaggcgcgt ggcgtcgtcg ccggcaaagc gctgccaact
     2521 cgccagcgcc gtcgggtcga tctccacctc atgaccctgc gcctgcaggc gccgcacgcc
     2581 aaggcggaag gcggcgcggt cacgcacggc gcctgagggg gaatagacgt agatgtgggc
     2641 catgatcggt cttctgaaaa ataacggctt gcggcgcttt ccaggcgcgc gccggcagct
     2701 atcaaaatta tagggctgcg gtcgatcctc gggcagcgcc gaagtgctgc agggcgcgtt
     2761 cagcgatggc gcgcccgtgc tcggacacga agtgccccgc ctcagggagc acccagggcg
     2821 gtgggcaacc gcgtatcagc gccgccagct ccgccatcac gggctgaccc agcaccgggt
     2881 cgcgcgcgcc aaccaccagc aggctgtgcc cgcgccactg atggcgccag aagtcgcgcg
     2941 cttggcgcga gatggcggcg ccatcctggt cggcatgcgc gggcaccagg cgcgggaagg
     3001 cgcgcgtggc ggcgcggtgc ccggcatcgg gaaacggggc cgcataggcg gcacattcgg
     3061 ccgcgctgag cgacggttcg gcacgcgcca gcaggcgcgc gatgtcgtaa gccggacgct
     3121 cggcacacca ctggcgccag tcgatgaagc cggccggcag tggcgcatcg cccgtcgcca
     3181 gcatggtgtt catggccagc aggcccaggt agcgcccggg cgccaccatc ggcagcgtca
     3241 gccccagcag gccgccccag tcctgcacca ccaggacgat gcgctgcaag tccagccgct
     3301 cgatgaattc gagcagcacc tgacgatgcc agtcgaagcg gtgcgtgccc tccttcttgg
     3361 gcttgtcgct gcggccgaag ccgatcaggt cgggcgtcac cacgcggtgc ccggcggcgg
     3421 caaatgccgc catcatgtgg cgatacagat agctccagcc cgggttgccg tgcaggcaca
     3481 gccaggtaag cggggcgtcc ggcgggcctt cgtccaccca gtgcaggcgc agcccggcca
     3541 gggctggcag atcgctcacg tagtgcggcg gccaggggtg gccgggcaag tcttggaagg
     3601 cggtatcggg cgtgcgcagg gcgtcgtcac gcagcgggtc gcggttgatc cgccgcggtt
     3661 tgaagaactg gcgcagcagc gccgcgcatt cgtgcgccag cacgccgccg cgcacctcgg
     3721 tttgatggtt gaggcgcgtt tcggcaaaca ggtccagcac cgaccccgca gcgcccgcct
     3781 tggggtcgct ggcgccaaac accacgcgat ccagccgggc atgcagcatc gcgccggcac
     3841 acatggcgca gggctcgagc gtcacgtaca gcgtgcagcc gtccagccgg tagttgccca
     3901 gcgcctgcgc cgccgcccgc agcgccacca cttcggcatg ggcggtcgga tcgtgcgcgc
     3961 cgatgggcgc gttgtggccg ctggcgatga cgcgaccatc cctgaccacg acagcgccca
     4021 ccggcacctc gcccagcgcc gccgcggcct gcgcctgctg cagcgccagg cgcatggcct
     4081 gatcgtcggt caggcggtcc tcgcccaccc gaataactcc tgtttttgta gctgctggcg
     4141 catgctctac aaggcctgat gctgaaaaac gttcacgaat cggcgtttga gccacagggg
     4201 tcgtccggac cgcctgacgg tgccgacgag ccgcccacca gcccgagcct gaccatccag
     4261 tcggccagcg cctgctgctg ggcgccgcca ccaaggtacg cggcgtgcag cgccgcatgg
     4321 gattccggac gatgctggtt gagcttgatc ttgcactgcc agtgatcgac ctgcagctcg
     4381 aaggccacga taccggccag cagcttgcgc gccagttcgc cttccatgtc gcgccattgg
     4441 gcggcatagg caggttcgtg atcaccgatc aggcatttca gcagtgcgtc cttggcgtgc
     4501 gcatcgtcgt ggtcgagcag gcgcgcggtc acgctgcaat gcacggcaac gtagttccag
     4561 ctgggtacgc gcgcccggtc cggatagacg ctgggcgaca tgtaggcgtg cggccccatg
     4621 aaggtgacca gcgcgcgtgc acgctgctgc aggtggcgcc attggacgtt gggccgggcc
     4681 atgtgaccga gcaggcgaaa acccgtgtct tcgtgctgca gatgcaaggg cagatgcgac
     4741 acgtacggca ggcccgcgtc gtcggtcgtg atcaggctgg ccagcgggtg ctcacgcatc
     4801 acccgggcgg ccaggcgctg gtcttgagtg gcgaattgcg agggcaggta catacgctgt
     4861 gacttgggga tcgaccctga caacccccgg tgggtaaccg tctgagggtt gcgctgatgg
     4921 tgtcgccagc gagccagacc gagaagccct ggccttacga gtggagtgca gctccattcc
     4981 caggtgagat ctcgatcacc gacgatggct cctacaccat gttcttcact cccactcgat
     5041 cgtcgccggc ggcttggaag atatgtcgta ggtgacgcgg ttgatgccgc gcacctcgtt
     5101 gatgatgcgg ctgctggtgc gcttgagcag ttcgtacggc agctcggccc aatcggcggt
     5161 catgaagtcg gaggtctgca ccgcgcgcag cgcgaccacg tggtcgtagg tgcgtccatc
     5221 gcccatcacg ccgacgctct tgacaggcag aaagacgccg aacgcctggc tggtcaggtc
     5281 gtaccagctc ttgcccgagt ggggctcgat ggtggagcgc agctcctcga tgaagatggc
     5341 gtcggcgtgg cgcagcaggt cggcgtattc ctgtttcact tcgcccagga tgcgcacccc
     5401 caggccgggc cccggaaacg gatggcgatg caccatctcc gcgggcaggc ccagcgccag
     5461 gcccaattcg cgcacctcgt ccttgaacag atcgcgcagc ggctccagca gcttcaggcc
     5521 gagctgttcc ggcagaccgc cgacgttgtg gtggctcttg atgaccaccg ccttcttgcc
     5581 cttgccgcca cccgattcga tgacgtcggg gtagatcgtg ccctgcgcca gaaagctcgc
     5641 ccccttgtgc cctgccccgc cggctttcag cttttcagcc tcggccttga agacgtcgac
     5701 gaacaggcgg ccgatgatct tgcgcttgtg ctccggatcg ctgacgcccc gcagcgcgtt
     5761 caggaacagc tcgctggcgt ccacgcgcac gacccgggcg tgcaggcggt cggcgaacat
     5821 ggccatcacc atgtcgcctt cgtgcaggcg cagcaggccg tggtcgacga acacgcaggt
     5881 cagctggtcg ccgatggcgc ggtggatcaa ggcggcggcg acgctcgaat cgaccccgcc
     5941 ggacagaccc aggatcacct cctcgtcacc cacctgagtc cgaatcgcct gcacggcctc
     6001 ctcgatgtga tcacgcatca cccagtcggg ccgggcgcca cagatctgaa ggacaaagcg
     6061 ctccagcatc gccctgccct gcaccgtatg ggtgacttcg gggtggaact gaagcgcata
     6121 gaagccacgc gcttcgtcgg ccatgccggc gatggggcaa ctgggcgtgc tggccatcag
     6181 cttgaagccg ggcggcaggg cggtcacctt gtcgccgtgg ctcatccaca ccttcagcat
     6241 gccatgccct tcgggcgtgg tgaagtcggc catgtccttg agcaggggcg tgtgcccatg
     6301 cgcgcgcacc tcggcgtagc cgaactcgcg cgtgtggccg ccttcgacct tgccgcccag
     6361 ctgctgcgcc atggtctgca tgccgtagca gatgcccagc accggaatgc ccagctcgaa
     6421 cacggcgtcg ggcgccttgt catcgacctc gtagacgctg gcgtggctgc ccgaaaggat
     6481 cacccccttg agcctgccgt cggcggcatg ctcgcgcacc cagtcgctgc tgacgtcgca
     6541 ggggtggacc tcgcagtaca catgggcctc gcgcacgcgg cgcgcgatca gctgggtgac
     6601 ctgggagccg aagtcgagga tgaggatctt gtcgtgtggc atgacgtttc gaagctaaat
     6661 tggcttctgg cgcttactgg accagcgccg atagctatca acaacatagc gcgcggccgg
     6721 ccgatggcct gcggcgtccg gctcaatcgg cgcgatagtt cggcgcttcc ttggtgatct
     6781 gcacgtcgtg cacatggctt tcgcggatgc cggcggcggt gatctgcacg aactccgccc
     6841 tggcgtgcat gtcatcgatg gtggcgcagc cgcaataccc catggccgcg cgcaggccgc
     6901 ccgcctgctg gaacatgatg gccaccacgc tgcccttgta gggcacgcga ccctcgatgc
     6961 cttcgggcac cagcttgtcg gcattcgggt tgcccgtgct gctttcctga aaatagcggt
     7021 cggcgctgcc ctgctgcatg gcaccgatgc tgcccatgcc gcgatagctc ttgtagctgc
     7081 ggccctggaa cagcacgatt tcgcccggcg cctcttcggt accggcaaac atgcccccca
     7141 tcatcacgct gtgcgcgccg gcggcaatgg ccttggcgat gtcgcccgag tagcgcacgc
     7201 cgccgtcggc gatcatgggc acgccggtgc cttccagcgc ctgcgatacg tcgtggatgg
     7261 cggtgatctg tggcacaccg acgccggcga cgatgcgggt ggtgcagatg ctgcccgggc
     7321 cgatgcctac cttgacgccg tctgcgccgg catccaccag cgcgcgcgcc gccttgccgg
     7381 tggcgatgtt gccgccgatc acatccacct gcggatactt gttcttcacc cagcgcacgc
     7441 gctcgatcac accctggctg tggccatggg ccgtatccac caccagggcg tcgacaccgg
     7501 ccttgaccag cgcctcgacg cgctcttcgg tgccctcgcc cacgcccacg gcggcgccca
     7561 cgcgcagccg gccatgggcg tcgcgcgcgg cgttgggaaa ggtggtctgc ttggtgatgt
     7621 ccttgaccgt gatcaggccc ttcaggcggt cctgctcatc caccaccagc agacgctcca
     7681 ggcggtgctt gttcaacagt gccttggcga catcgagggt ggtgccctcg ggcacggtga
     7741 tcagcttgtt gcgcggggtc atgatttcgc gcaccggcac gtcgtagcgc gtttcaaacc
     7801 gcagatcgcg cccggtgacg atgcccacca cccggccatg gtcgatgacg ggaaagccgc
     7861 tgatgtccag ttcgtccgac agctgcatca cctgcagcac cgtgtgctcg ggcgtgatca
     7921 ccaccggatc gcgcagcaca ccgctttcgt aacgcttgac cttggccacc tgcgtggcct
     7981 gctcgtgcgc cgtcagattc ttgtgcacga tgccgatgcc accctcctgg gccatggcga
     8041 tggccaggcg cgcttcggtg acggtgtcca tggcggcaga caccagcggc aggttcaggc
     8101 ggatgcggcg ggtgaactgg gtggagaggt tgacgtcctt gggcaggacc tgggagtacg
     8161 ctggcaccag caacacatcg tcgaaggtca gcgcttggcc gatcaggcgc atgggatagg
     8221 ctccaaaaga cggattgtaa acagcgccga cgcagcatct ttccatatgc agggcgtagg
     8281 gggaagcgcg tgtcaaggtc aggtaaagcc aggtgagcag acgcaggcgc gtcggcgcca
     8341 gggtggggcc acgcgttgca acaggcgcga ggctgccgcc ttagcctgtt caaggagttg
     8401 ccgacatgac cctgacaagc cacacgcctt ctcgccgtgc cctgctcgcc ggatgcctgg
     8461 caggggccac ggcgctggcg ggttgcatgg tggcgccggt gcccggcgcg gtctacacgg
     8521 accgcgacgg tgtggtcacc tcgaccacgg tgtacgcgcc ggtggcgcca ccggcgccct
     8581 actacgaagt gcagccggcc ctgccgtatc ccggcgcggt gtggatcggc ggctactggc
     8641 gctggggcgg gcaccgccat gtctgggtgc ccggtcgcta tgaacgcccc cgccccggtc
     8701 accactatgt gccgcaccgc tggcaccggt cgccgcacgg cggctggtat ctgcaaggcg
     8761 gcgcatggat gcgatgacca agccccgagg ctcatcggaa ctgcgtcaag agctttcttt
     8821 tcgatagcaa actggcacgg gcacaaccac tgcccgccgc cgcctgtgaa gccgcgagac
     8881 ggaacgtcgc cgccgacgcc ggctctgcca gcgccttcgg gttcgcccat gagcgaaaaa
     8941 agccacggca tgccgtggct tttcctgtct cctcaccgac cggcacgcac cggtcggcct
     9001 tcaagcgcgg atcagtggaa ctgctcttcc tcggtcgaac ccgtcagggc cttcacgctg
     9061 gaggagccgc cctggatcac ggtggtcacg tcgtcgaagt agcccgcgcc cacttcct
//
LOCUS       DAOOOG010000101         9088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_8930_length_9088_cov_24.136389, whole genome shotgun sequence.
ACCESSION   DAOOOG010000101 DAOOOG010000000
VERSION     DAOOOG010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8930_length_9088_cov_24.136389"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PK242_08495"
     CDS             complement(<1..133)
                     /locus_tag="PK242_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HOZ94097.1"
                     /translation="MSNVFIAFQKNEDSRAIVNAIVADNPTAIVSDQPAMVKIDVPGR
                     "
     gene            complement(168..1166)
                     /locus_tag="PK242_08500"
     CDS             complement(168..1166)
                     /locus_tag="PK242_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene monooxygenase hydroxylase
                     subunit beta"
                     /protein_id="HOZ94098.1"
                     /translation="MQIDLRTVSITPLRQTYNHIAERLGADKPASRYIEGTMDVQANA
                     NFHYRPTWDPQHELFDPRRTRIVMQDWYALKDPRQLYYGTYTQARARMQETAEADFEF
                     VESRGLAERFDDAARRTALDFYVPLRHVAWGANMNGAYQCAYGYGTAITQPCLYAGMD
                     QLGIAQYLTRLGLLLGNQGDLEAGKHAWLNAPAWQGLRRLVEDTWVLKDWFELFVAQN
                     VVQDGVLFGLAYKEVDQAISEKAGPTVSMLTRFQAEWFQDASKWVDAVIKAAASESAD
                     NQALLSQWYGQWRARVQEALAPVAQLALGEQGAQALERAVAAVDARMVKAGLTLSA"
     gene            complement(1208..1498)
                     /locus_tag="PK242_08505"
     CDS             complement(1208..1498)
                     /locus_tag="PK242_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase subunit"
                     /protein_id="HOZ94099.1"
                     /translation="MADAPTPSDDRWDVQRRFIRIVEHHDNGMVEFEFAVGEPGLFVE
                     MVMPRAEFEEFCATQGVQPTEGRLPSRDGGSDAHEWDWSLRDARDRHFRHEP"
     gene            1716..3458
                     /locus_tag="PK242_08510"
     CDS             1716..3458
                     /locus_tag="PK242_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOZ94100.1"
                     /translation="MVFIDNRAVSELPPLPSTADLRRQVEFSSSDGRIWLAGQRMLLM
                     HAASLGTLRRELIQSIGPAAARRLLLRVGYASGERDAALARQLRADADMFSLFAVGPQ
                     LHMLEGAVQVTPEQLELDVAAGQFHGVFRWDHSWEVETHLRDFGPQHEPVCWTLLGYA
                     SGYTSAFMGRPVLYKEVACAACGHAHCRIEGRPVTDWPDGEQLASDYAAQSLTLDPPD
                     GAIGAWPAEVPADDQPETPVDALGPLIGQAPGFSAATQLLRKAAGTQVTVLLTGETGT
                     GKERFARALHALSPRAAKPFVAVNCAALPGELIESELFGTEKGAYTGADAARAGRFER
                     AHGGTLFLDELGELPLPAQAKLLRVLQDGQVERLGSSQPRHVDVRLVAATHVDLMQAV
                     RAGRFRQDLYYRINVYPIRIPPLREREGDIEPLARYLLQRFTLLHDKHVKGFADRALH
                     ALRTYHWPGNVRELENLIERGVILATAGQTMQVEHLFPEAGAPPGAAPTHTLAASGRL
                     REVPPPAAPDAHPWIDQLLTHQIGLTALEQALIEQAVQRSRGNLAAAARLLGLTRPQL
                     SYRLSKLRGERPST"
     gene            3478..4260
                     /locus_tag="PK242_08515"
     CDS             3478..4260
                     /locus_tag="PK242_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOZ94101.1"
                     /translation="MAKRSALPSGDQPLSASRTLIERAYEQLRDDIVDGHLPPDEKLR
                     VEHLKARYGVSAGTLREAITRLVSDALVITEGQRGFRVAPIAVADLQDLTNLRVHIEI
                     DALRQSMRQGGADWRQRVQAAYLDMSQLEQPLRPGHAKSWEALNIAFHEALLAGHDSP
                     WTLRLLRTLARQSERYRRYAISLPESRRDVHAEHREIYDSAMNGNELRAALALEAHIR
                     TTAELVARALHGNGHAPSTVRSTGPNGAGAVAPGSNGFAADR"
     gene            complement(4577..5095)
                     /locus_tag="PK242_08520"
     CDS             complement(4577..5095)
                     /locus_tag="PK242_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOZ94102.1"
                     /translation="MPAVRVRIQTEDFDLAAEVAALRAGDARVGAVCAFVGTVRDRHA
                     PALAAAPHADGGASPVSALELEHYPGMTEKAIEAMIDAAQQRFDILGARVVHRVGLLQ
                     PLDQIVLVAVTSMHRHESFAACEFIMDYLKTQAPFWKKESTPEGAHWVDARVADDAAL
                     ARWGIRAGNAAR"
     gene            complement(5101..5394)
                     /locus_tag="PK242_08525"
     CDS             complement(5101..5394)
                     /locus_tag="PK242_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOZ94103.1"
                     /translation="MNAPDRAQAQQPLRTVTVRYFAAIREALGTGSERLTTPAATLSA
                     LRDELIARGGAHAETLARGKAVRMALDQLMVSDEAAPLADGAEVAFFPPVTGG"
     gene            complement(5391..6644)
                     /locus_tag="PK242_08530"
     CDS             complement(5391..6644)
                     /locus_tag="PK242_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOZ94104.1"
                     /translation="MSDTPRRPPLMPLDDALARLLAAAAPLGRTEPVDTFDADGRVLA
                     QDLVSALQVPPQDNSSMDGYAVRSADVTAAGVELPVSQRIAAGHAGAPLAPGSAARIF
                     TGAPMPAGADAVVMQEDAESLDDGARVRLNAVPAPGQWVRAAGEDVTRGRAVLQQGER
                     LAPASIGLAAGIGMASLQVAARPRVALFSTGDELVMPGAVPPEAMKPGAIYNSNRFFL
                     RALLQRLGCTVTDLGIVPDDRAATLEALRQAADGHDLILTSGGVSVGEEDHIKPAVEQ
                     LGALDLWQIAMKPGKPFAYGHVRRAGGGQAHFMGLPGNPVSSYVTFLLLVRPFLLRLQ
                     GAREVALPSLALRADFTLDKADRRREFLRARRNAQGGLELFGNQSSGVLTSAHWADGL
                     IDLPAGQTIAAGDMVRFIPVRELLA"
     gene            complement(6637..7179)
                     /gene="mobB"
                     /locus_tag="PK242_08535"
     CDS             complement(6637..7179)
                     /gene="mobB"
                     /locus_tag="PK242_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOZ94105.1"
                     /translation="MKVVGFAGYSGSGKTTLVEQLIARLRARGLRVSVVKHAHHRFDI
                     DHPGKDTFRHREAGAFEVAIASSRRLALMREFERPTELSVHHLIAELWDGVDWVLVEG
                     FRGSDLLKVEVWRPATGHPARYPDDDFVVAIATDAPAALPVPTLRPVLDLNDAEAIAQ
                     WLIDNGERFDYDPERYLPHV"
     gene            complement(7250..8677)
                     /gene="thrC"
                     /locus_tag="PK242_08540"
     CDS             complement(7250..8677)
                     /gene="thrC"
                     /locus_tag="PK242_08540"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOZ94106.1"
                     /translation="MLYLSTRGDRTPRRFCDILLEGLAPDGGLYLPEHYPQIDAARLA
                     ALRRTHAEGGYAALAFEILSLYIDDIPADDLRAICQRTYTAEVFGTPEIVPLKPLHQG
                     FYLEALSNGPTLAFKDMAMQLLGQLFEYELGRRGQTLNILGATSGDTGSAAEYAMRGK
                     RGIRVFMLSPHGRMSAFQQAQMFSLQDPNIHNIAIEGVFDDCQDIVKAVSNDLEFKRK
                     YRIGTVNSINWARLLAQVVYYFAGYFQATRSDGERVSFTVPSGNFGNVCAGHVARQMG
                     LPIAQLVVATNENDVLDEFFRTGVYRVRGAADTHETSSPSMDISKASNFERFVFDLLG
                     RDGARCKALFDDALRQHGQFDLSGDARFADAAARYGFVSGRSTHADRLATIRRVFAEH
                     GVTIDTHTADGVKVAREHLQPGVPMIVLETALPIKFAATIEEALGQPPARPARFDGIE
                     QLPRRVRVLPADAAQVKALITAECE"
     gene            complement(8718..>9088)
                     /locus_tag="PK242_08545"
     CDS             complement(8718..>9088)
                     /locus_tag="PK242_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOZ94107.1"
                     /translation="RLATSDPQHRVPHLAFQPGQMRDVGVLSMEQVVTGYYLRLRVAD
                     ETGVLARVATILAEAGISIDALLQREADEVGGEGSTQTDLIILTHDAREGAMNQALER
                     LQALPSVLAPIVRIRKEELN"
BASE COUNT         1366 a         3169 c         3167 g         1386 t
ORIGIN      
        1 tgcggccggg cacgtcgatc ttgaccatgg ccggctggtc gctgacgatg gccgtggggt
       61 tgtcggccac gatggcgttg acgatggcgc gcgaatcctc gttcttctga aaggcgatga
      121 atacgttgga catgccttga ctcctgtgct gtgttcgggg gtggtggtca ggcgctcagc
      181 gtcaggccgg ccttgaccat gcgggcgtcg acggcggcca cggcgcgttc cagcgcctgg
      241 gcaccctgct cgcccagcgc cagctgcgcc accggggcca gcgcctcctg cacgcgggcg
      301 cgccactggc cgtaccactg gctgagcagc gcctggttgt cggcactttc gctggcggcg
      361 gccttgatga cggcgtcgac ccacttgctg gcgtcctgga accactcggc ctgaaagcgc
      421 gtgagcatgg atacggtggg accggccttt tccgagatcg cctggtccac ctccttgtag
      481 gccagaccga acagcacgcc gtcttgcacc acgttctgcg ccacgaacag ctcgaaccag
      541 tccttgagca cccaggtgtc ctcgaccagg cggcgcagac cctgccaggc aggcgcattc
      601 agccaggcgt gcttgccggc ttccaggtcg ccctggttgc ccagcagcag acccaggcgg
      661 gtcaggtact gggcgatgcc cagctgatcc atgccggcgt acaggcaggg ctgggtgatg
      721 gccgtgccgt agccgtaggc gcactggtag gcaccgttca tgttggcgcc ccaggcgacg
      781 tggcgcagcg gcacatagaa gtccagtgcg gtgcggcggg cggcgtcgtc gaagcgctcg
      841 gccaggccgc ggctttcgac gaactcgaaa tccgcctcgg cggtttcctg catgcgggcg
      901 cgcgcctggg tgtaggtgcc gtagtacagc tggcgcgggt ccttcagcgc ataccagtcc
      961 tgcatcacga tccgggtacg ccgagggtcg aacagctcat gctgcggatc ccaggtgggg
     1021 cggtagtgga agttggcgtt ggcctgcacg tccatcgtgc cttcgatgta gcgggaggcc
     1081 ggcttgtcgg cacccagccg ctcggcgatg tggttgtagg tctgccgcag cggggtgatg
     1141 ctgacggtgc gcaggtcgat ctgcatggga cgtgtctcct tggcctggtg gtttgaacgg
     1201 atggaaatca gggctcgtgg cggaagtgcc ggtcgcgggc atcgcgcagg ctccagtccc
     1261 actcgtgggc gtcgctgccg ccgtcgcgtg aaggcaaacg accttcggtg ggctgcaccc
     1321 cctgggtggc acagaactcc tcgaactcgg cgcgcggcat caccatttcg acgaacagcc
     1381 cgggttcgcc gaccgcgaac tcgaactcga ccatgccgtt gtcgtggtgc tccacgatgc
     1441 ggatgaagcg gcgctggacg tcccagcggt cgtcggatgg ggtgggtgca tcggccatgc
     1501 cgccatccct tgcacggtgc atgccaaacc ataggcaatc aggaaaaatc tcgataattc
     1561 cagggaaaac ccctaaacag aaccatccac cgacaccgag caccacccct tcacgcgggt
     1621 cgcaagcgtg aaatactgcg ctgcagcatc acgaaatcaa gcattcatca actggccgat
     1681 tcacgatttt gatccgacca ccctcttgca caaagatggt ctttatcgat aatcgagccg
     1741 tgtccgagct gccacccctg ccctccaccg ccgatctgcg ccgccaggtg gagttctcgt
     1801 cgtccgatgg ccggatctgg ctggccggcc agcgcatgct gctgatgcat gcggcctcgc
     1861 tggggacctt gcggcgcgag ctgattcagt cgatcggccc ggccgccgcg cggcgcctgc
     1921 tgttgcgcgt cggttacgcc tcgggcgagc gcgacgcggc gctggcgcgc cagctgcgcg
     1981 ccgacgccga catgttttca ctgtttgccg tcggccctca gctgcacatg ctggagggcg
     2041 cggtgcaggt cacgccggaa cagctggagc tggacgtcgc ggccggccag ttccacggcg
     2101 tgttccgctg ggaccattcg tgggaggtgg aaacccactt gcgcgacttc ggcccccagc
     2161 acgagcccgt ctgctggacc ctgctgggct acgcctcggg ctacaccagc gccttcatgg
     2221 gccgcccggt gctgtacaag gaagtggcct gcgccgcctg cggtcacgcg cactgccgca
     2281 tcgagggtcg gccggtgacc gactggcccg acggcgagca gctggccagc gactacgccg
     2341 cgcagtcgct gacgctcgat ccgccggacg gcgccatcgg cgcctggccc gccgaagtgc
     2401 cggccgacga ccagcccgaa accccggtgg acgccctggg cccgctgatc ggccaggcgc
     2461 ccggttttag cgccgccacg cagttgctgc gcaaggcggc gggcacccag gtgacggtgc
     2521 tgctgacggg cgagaccggc accggcaagg agcgctttgc gcgcgccctg catgcgctca
     2581 gcccgcgcgc ggccaagccg tttgtcgccg tcaactgcgc ggcgctgccg ggcgagctga
     2641 tcgaatcgga gctgttcggc accgagaaag gcgcctacac cggcgccgac gccgcccgcg
     2701 ccggccgctt cgagcgcgcc cacggcggca ccctgttcct cgatgagctg ggcgagttgc
     2761 cgctgccggc ccaggccaag ctgctgcgcg tgctgcagga cggacaggtc gaacgtctgg
     2821 gctcgtcgca gccgcggcat gtggacgtgc ggctggtcgc ggccacccac gtggacctga
     2881 tgcaggcggt gcgtgccggg cgcttccggc aggatctcta ctaccgcatc aacgtctacc
     2941 cgatccgcat cccgccgctg cgcgagcgcg agggcgacat cgaaccgctg gcgcgctacc
     3001 tgctgcagcg cttcacgctc ctgcacgaca agcatgtcaa ggggttcgcc gaccgcgccc
     3061 tgcacgcact gcgcacctac cactggcccg gcaacgtgcg cgagctggaa aacctgatcg
     3121 aacgcggcgt gatcctggcc acggccggcc agaccatgca ggtcgaacac ctgttccccg
     3181 aggccggcgc accgcccggc gccgctccga cccacacgct cgcagcctcc gggcgcctgc
     3241 gcgaagtgcc tccgcccgcc gcgcccgacg cccacccatg gatcgatcag ctgctgacgc
     3301 accagatcgg cctgacggca ctggagcagg cactgatcga gcaggccgtg cagcgcagcc
     3361 gcggcaacct ggcggcggcc gcgcgcctgc tggggctgac ccggccgcag ctcagctacc
     3421 gactgagcaa gctgcgcggc gaacggcctt ccacctgacc ccggcagtgg agactcgatg
     3481 gccaaacgct ctgccctgcc ctctggcgac cagcccctgt cggcgtcacg cacgctgatc
     3541 gagcgcgcct acgagcaact gcgcgacgac atcgtggacg gtcacctgcc gcccgacgag
     3601 aagctgcgcg tcgaacacct gaaggcgcgc tatggcgtga gcgccggcac actgcgcgag
     3661 gccatcaccc ggctggtcag cgatgcgctg gtgatcaccg agggccagcg cggttttcgc
     3721 gtggccccga tcgccgtcgc cgatctgcag gatctgacca acctgcgcgt gcacatcgag
     3781 atcgacgccc tgcgccagtc gatgcggcaa ggcggcgccg actggcgcca gcgggtgcag
     3841 gcggcgtacc tggacatgtc ccagctcgag caaccgctgc gccccggcca cgccaagtca
     3901 tgggaagcgc tgaacatcgc ctttcacgag gcgctgctgg ccggccacga ttcgccctgg
     3961 acgctgcgcc tgctgcgcac cctggcacgg cagagcgaac gctaccgccg ctacgccatc
     4021 agcctgccgg agagccgacg tgacgtgcat gccgagcacc gcgagatcta cgacagcgcg
     4081 atgaacggca acgagctgcg cgccgcactg gcgctggaag cgcacatccg caccacggcc
     4141 gagctggtgg cgcgggcgct gcatggcaac ggccacgcgc catcgacggt ccgatcaact
     4201 ggcccgaacg gcgccggcgc cgtcgcgcca ggctccaacg gtttcgccgc cgaccgctga
     4261 cccctgcccc tcgcgtcatc gaagcgcagc gaggtcattc gccattcgtc atcctagaaa
     4321 cggcagttgg acgcgcccgc ccgtgccgtg atcggcgtgc caggcaagac gcgacgccgc
     4381 cgcgggctgg tgcccgcaag aaggaccaac gccgcatggc gcggcgagcg cggtgcgggt
     4441 gggtgcgtag cgtgctcacc caagaggtgg tggcgatcgc agccgcgaga ccccgcatcg
     4501 atgcggcccg cccgaagttg accagcggtc aactgccgtt tctagggtca tcggtggtcg
     4561 caggcgcaag cccgcatcaa cgcgccgcgt tgcccgcgcg gatgccccag cgcgccagcg
     4621 ccgcgtcgtc ggcgacgcgc gcatcgaccc agtgcgcgcc ctcgggcgtg gactcttttt
     4681 tccagaacgg cgcctgggtc ttgaggtagt ccatgatgaa ttcgcaggcg gcaaagctct
     4741 cgtgccggtg catggacgtg acggccacca gcacgatctg atcgagcggc tgcagcagcc
     4801 ccacccggtg caccacgcgc gcgcccagga tgtcgaagcg ctgctgcgcg gcgtcgatca
     4861 tggcttcgat ggccttttcg gtcatgccgg ggtagtgttc cagctcgagc gccgacaccg
     4921 gcgacgcgcc gccgtcagcg tgcggcgccg ccgccagcgc gggggcatgg cgatcgcgca
     4981 ccgtgccgac gaaggcgcag acggcgccca cgcgggcgtc gccggcgcgc agcgcagcca
     5041 cctcggccgc gaggtcgaaa tcttcggtct ggatgcggac gcgcacggcg ggcatggcgc
     5101 tcacccgccg gtcactggcg gaaagaaggc cacctcggcg ccgtcggcca gcggcgccgc
     5161 ttcgtcgctc accatcagct ggtcgagcgc catgcgcacg gccttgccgc gcgccagcgt
     5221 ctcggcatgg gcgccgccgc gcgcgatcag ctcgtcgcgc agcgcgctca gcgtggcggc
     5281 tggggtggtc aggcgttcgc tgccggtgcc cagcgcctcg cggatggcgg cgaaatagcg
     5341 cacggtgacg gtgcgcagcg gttgctgcgc ctgggcgcgg tccggtgcgt tcatgccagc
     5401 agctcccgca ccgggatgaa gcgcaccatg tcgccggcgg cgatggtctg gccggccggc
     5461 agatcgatca ggccgtcggc ccagtgggcc gaggtcagca cgcccgagct ctggttgccg
     5521 aacagctcca gcccgccctg ggcgttgcgc cgcgcacgca ggaattcgcg ccgccggtcg
     5581 gccttgtcca gcgtgaagtc ggcgcgcagc gcaagcgacg gcagcgccac ctcgcgcgcg
     5641 ccctgcagcc gcagcaggaa cggccgcacc agcagcagaa aggtgacgta gctggacacc
     5701 gggttgcccg gcaggcccat gaaatgcgcc tggccgccgc ccgcgcgccg gacgtggcca
     5761 taggcgaagg gcttgccggg cttcatggcg atctgccaca gatccagcgc gcccagctgt
     5821 tcgacggcgg gtttgatgtg gtcttcctcg ccgacgctga cgccgccgct ggtgaggatc
     5881 aggtcgtgcc catcggcggc ctggcgcagc gcctccaggg tggcggcacg atcgtcgggc
     5941 acgatgccca ggtcggtcac ggtgcaaccc aggcgctgca gcagcgcgcg cagaaagaag
     6001 cggttgctgt tgtagatggc gcccggcttc atggcctcgg gcggcaccgc accgggcatg
     6061 accagctcgt cgccggtgga aaacagcgcc acccgcgggc gcgccgccac ctgcagcgac
     6121 gccatgccga taccggccgc cagcccaata ctggcgggcg ccagacgctc cccttgttgt
     6181 agcacggcgc ggccgcgtgt gacgtcctcg ccggccgcgc gcacccattg gccgggcgcg
     6241 ggcacggcgt tcaggcgcac ccgcgcacca tcgtccagcg actcggcgtc ttcctgcatc
     6301 accaccgcgt cggcgccggc cggcatgggc gcgccggtga agatgcgcgc cgcactgccc
     6361 ggtgccagcg gcgcgcccgc atggccggcc gcgatgcgct ggctgaccgg cagctccacg
     6421 ccggccgcag tcacgtcggc actgcgcacc gcgtagccgt ccatcgagct gttgtcctgc
     6481 ggcggcacct gcagcgccga caccaggtcc tgcgccagca cgcggccatc ggcgtcgaag
     6541 gtgtcgaccg gttcggtgcg ccccagcggc gcggccgccg ccagcaggcg ggccagcgcg
     6601 tcgtccagcg gcatcagggg cggtcggcgc ggggtgtcag acatggggca ggtaccgttc
     6661 ggggtcgtag tcgaaacgct caccattgtc gatcagccat tgggcaatgg cctcggcatc
     6721 gttcagatcc agcaccgggc gcagggtcgg cacgggcagc gcggccggtg cgtcggtggc
     6781 gatggcgacg acgaagtcat cgtccggata gcgcgcaggg tggccggtgg cggggcgcca
     6841 gacctcgacc ttgagcaggt cgctgccgcg aaaaccctcc accagcaccc agtccacgcc
     6901 gtcccacagc tcggcgatca ggtggtgcac cgacagctcg gtcgggcgct cgaactcgcg
     6961 catcagcgcc agccgccggc tggaggcgat cgccacctcg aaggcgccgg cctcgcggtg
     7021 gcggaaggtg tccttgcccg ggtggtcgat gtcgaagcgg tgatgcgcat gcttgaccac
     7081 cgacacgcgc agaccgcgcg cgcgcagccg cgcaatcagc tgctcgacca gtgtggtctt
     7141 gcccgagccg gagtagccgg caaagcccac gaccttcatc gcttggtcac ccgaatacta
     7201 tcaatatgat agctgctggc gcttgtcagt tgtgcgccag aggccgattt cattcacatt
     7261 ctgcggtgat cagcgccttc acctgagcgg cgtcggcggg caacacgcgc acgcgccggg
     7321 gcagctgctc gatgccgtcg aaccgggcgg gccgcgcggg cggctggccc agcgcctctt
     7381 cgatggtggc ggcaaacttg atcggcagcg cggtctccag cacgatcatc ggcacgccgg
     7441 gctgcaggtg ctcgcgcgcc accttcacgc cgtcggcggt gtgggtatcg atggtcacgc
     7501 cgtgctcggc aaacacgcgg cggatggtgg ccaggcgatc ggcgtgggtg ctgcggccac
     7561 tgacaaaacc gtagcgcgcg gcggcgtcgg caaagcgggc gtcgccgctc aggtcgaact
     7621 ggccgtgctg gcgcagcgcg tcgtcgaaca gcgccttgca gcgcgcgccg tcgcgcccca
     7681 gcaggtcgaa cacgaagcgc tcgaagttgc tggccttgct gatgtccatg ctggggctgc
     7741 tggtctcgtg ggtgtcggcg gcgccgcgca ctcggtagac gccggtgcgg aagaactcgt
     7801 cgagcacatc gttttcattg gtggccacca ccagctgcgc gatcggcagc cccatctggc
     7861 gcgccacgtg gccggcgcag acattgccga agttgccgct gggcactgtg aagctgacgc
     7921 gctcgccgtc cgagcgcgta gcttggaagt aaccggcgaa gtagtacacc acctgcgcca
     7981 gcagccgcgc ccagttgatg gaattgacgg tgccgatgcg gtacttgcgc ttgaactcca
     8041 gatcgttgct gaccgccttg acgatgtcct ggcagtcgtc gaacacgcct tcgatggcga
     8101 tgttgtggat gttcgggtcc tgcaggctga acatctgcgc ctgctgaaac gcgctcatgc
     8161 gaccgtgcgg gctgagcatg aacacgcgga tgccgcgctt gccgcgcatg gcgtattcgg
     8221 cggcgctgcc ggtgtcgcca ctggtggcac ccagaatgtt gagcgtctgg ccgcgccggc
     8281 ccagctcgta ctcgaacagc tggcccaaca gctgcatcgc catgtccttg aaggccagcg
     8341 tggggccgtt ggacagcgcc tccaggtaaa agccctggtg cagcggcttc agcggcacga
     8401 tctcgggcgt gccgaacact tcggcggtgt aggtgcgctg gcagatcgcg cgcaggtcgt
     8461 cggccgggat gtcgtcgatg tagagcgaca ggatctcgaa ggccagcgcc gcgtagccgc
     8521 cttcggcatg ggtgcggcgc agcgccgcca gccgggcagc gtcgatctgc gggtaatgct
     8581 cgggcaggta caggccgcca tcgggcgcca ggccttccag caggatgtcg cagaagcggc
     8641 gcggcgtgcg gtcgccgcgg gtggacagat aaagcatggt tcgctcgggc ggtgtcacgc
     8701 ggcacgggcc gcggcgttca gttcagctct tccttgcgga tgcgcacgat cggcgccagc
     8761 acgctgggca gggcctgcag ccgctccagt gcctggttca tggcgccctc gcgcgcatcg
     8821 tgggtgagga tgatcaggtc ggtctgggtg ctgccttcgc cgcccacctc gtcggcctcg
     8881 cgctgcagca gcgcgtcgat gctgatgccg gcctcggcca ggatggtggc cacgcgcgcc
     8941 agcacgccgg tttcgtcggc cacacgcagg cgcaggtagt agccggtcac cacctgctcc
     9001 atcgacagca cgccgacgtc gcgcatctgc cccggctgga aggccaggtg cggcacgcgg
     9061 tgctgcgggt cgctggtggc caggcggg
//
LOCUS       DAOOOG010000102         9043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_8983_length_9043_cov_33.261571, whole genome shotgun sequence.
ACCESSION   DAOOOG010000102 DAOOOG010000000
VERSION     DAOOOG010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8983_length_9043_cov_33.261571"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1193)
                     /locus_tag="PK242_08550"
     CDS             complement(6..1193)
                     /locus_tag="PK242_08550"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOZ94108.1"
                     /translation="MSNATAMGVLAGVRVLELGQVLAGPFAGAIFADLGAEVVKVERV
                     EGGDDARAMGPAFRHGDALNFHIFNRGKQSVALDLKSADGLAAFERLVGEADIFIHNL
                     RPGVPAKLGIDGASLCARHPRLIYCEISAFGSTGPMAMQPGYEPLVQAFSGLSSINGG
                     PDDPPMRSGASMCDQGSGMWAVIGALALLQRRQQTGRGGILQTSLLETALVWNGQKAD
                     AYVNEGRLPLRDRSGHPGFVPYEAFDTQDAPLLICCGNDRLFAKLATELGRPDWLADE
                     RFASNRARLAHKAELLAQLQPLLRARPRAEWAARFEAAGVPCAPIHSVPEALAHPQVQ
                     ALGILAPLPGEDFALTALPFSIDGARPLPRSAAPRLGADNAAHGLPPATVTPFTQNPQ
                     ETP"
     gene            1291..2202
                     /locus_tag="PK242_08555"
     CDS             1291..2202
                     /locus_tag="PK242_08555"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ94109.1"
                     /translation="MRLDLSLRQLEAFVQVADSGSFRRAAARLGQAQPVLSRLIGQAE
                     QTLGARLFDRDTRRVEITPAGRELLPLAQRLLRDFDDTLGDFGAFMAGRSGRVSMVVL
                     PSVSVAWVPRAMAAFARTHPQVNFALAEAPADTLLALLEAGRADFGISVRPAPQQRLH
                     YRHLQDDPFVLVCRRDDPLAARAAVPWSVFGMRDCIMSAAHSSIRQVTDAVFLRQRTP
                     PSPVLEYPSVAAAGALVAAGLGITALPVLALQLVDMRELVAVPLVRPAMARPIGIVTR
                     IGRSLSPATQAFMASLQAAPAGAPDAP"
     gene            complement(2153..3478)
                     /locus_tag="PK242_08560"
     CDS             complement(2153..3478)
                     /locus_tag="PK242_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOZ94110.1"
                     /translation="MNALTLFLPCAGGVEDFLADEVHGLSGLVGEDLLSLRGGVRVRG
                     DWALMQRLNLHSRLAQRVLIELAHGPYQSEQDLYALAAGVPWEQWFTPRQRFRVDLTA
                     QASPLKSLNFATLRIKDAVADRFRQQAGGVRPSIDTQQPDVRVQAHLGPQDATLYIDT
                     SGEPLFKRGWRQDKGDAPLKETLAAAMLAACGWWQPATRSVSPLPLYDPCCGSGTIVI
                     EAAQLLLGLPAGGQRRFAFEQLRPHDPAGWNAIKTEAAGACSTSAGDQNDAQTPAVRI
                     FGSDVAHRMVDFAQRNARRAGVAAAVQLRGGDALQRLPPCAEPGVLLLNPPYGERIAA
                     AGVAGARGGREAFQSGTLARQRAGGAAPADDVNAGDDFFARLAAHWKSHYAGWSAWLL
                     TPDSKLPGRLRLKESRRVPLWNGPIECRLLRFDLTARRAPPPAPPAATP"
     gene            complement(3475..3921)
                     /locus_tag="PK242_08565"
     CDS             complement(3475..3921)
                     /locus_tag="PK242_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOZ94111.1"
                     /translation="MARIVFDLPAQFGFATELQIYISHVNQGGHLDNAQLLALVSEAR
                     LRFFHALGYREGHVDGAAIVVGDMLAQYKSEAFHGETLRVQMQPADFNRYGFDLVFRM
                     TEVSQGREVARGKTGIVFIDPVSRRVTPIPAPIQGKLLACAAAATP"
     gene            complement(3936..5483)
                     /gene="rpoN"
                     /locus_tag="PK242_08570"
     CDS             complement(3936..5483)
                     /gene="rpoN"
                     /locus_tag="PK242_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOZ94112.1"
                     /translation="MKPGLSLRVAQQLALTPQLQQSIRLLQLSTLELSGEVEQMLADN
                     PFLERDDEAGDAAAPTPADRGERTDENAIDSGAAGADSTSAEADFDAEPLTPDDWDGD
                     GSVDIAPDDAEWGGEAPARQREAGDDDTDPGERVGGQESLAEHLLRQALTLRLAPEDA
                     AALRFLIGSLNDDGYLEDSLAQLAATLTDADDELEQMQELVHRLTLALKLLQSLEPAG
                     VGARHLAECLTLQLRSRQAERALAPQEQARIATALRICARHDAVELLARRDSRKLAQL
                     VGESEDAVRAAMALIGRLDPKPGRRFADVERQIVVPDVIVSAVGTAERPRFRVALNPD
                     VVPRLRVHEQYASALRGSDKHPALSERLQEARWFIRNIQQRFDTILRVANAIVLRQRD
                     FFIHGELAMRPLIQRELADELGVHESTISRVTTAKYMATPRGTFELKYFFGSALGTES
                     GSHASSTAVRALIRQLIEAEDRARPLSDAQIAEQLKAQGIDCARRTVAKYREALRLPT
                     ASLRKSH"
     gene            complement(5480..6274)
                     /gene="lptB"
                     /locus_tag="PK242_08575"
     CDS             complement(5480..6274)
                     /gene="lptB"
                     /locus_tag="PK242_08575"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOZ94113.1"
                     /translation="MASRFDAPAAADAPRAHGQSRLQVRGLRKNYGARRVVHDVSLDV
                     VNGEVVGLLGPNGAGKTTSFYMIVGLVRADAGSIAIDDQPIEHMPIHRRSRLGLSYLP
                     QEASIFRKLTVAENVRAVLELQVDAQGRPLGRDAIEQRLNELLQQLHVEPLRDSPAPA
                     LSGGERRRVEIARALATQPRFILLDEPFAGIDPIAVIEIQRIIAFLKSQGIGVLITDH
                     NVRETLGICDHAVIISEGRVLATGTPADIVANPEVRRVYLGEHFRL"
     gene            complement(6307..6948)
                     /gene="lptA"
                     /locus_tag="PK242_08580"
     CDS             complement(6307..6948)
                     /gene="lptA"
                     /locus_tag="PK242_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HOZ94114.1"
                     /translation="MKSSVPPFAPSLRLLCATLLLGLAAPAWAERADRDKPMNIEADA
                     LRYEEQKQQSTFTGKVLVTKGTIVMRGARLEVRQDSAGNQFGTLSGEPGKRAFFRQKR
                     EGVNEFIEGEGETIEYDGKTDTVRLIRRAEMRRLEGATQLDQVLGSLIVYNNRTEVYT
                     VDGAPAAGAPSAAPGGRVRATLAPRQAASAASAASAPALQLRPAAALPEGSGQ"
     gene            complement(7075..7704)
                     /locus_tag="PK242_08585"
     CDS             complement(7075..7704)
                     /locus_tag="PK242_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HOZ94115.1"
                     /translation="MNRREFTLAGALALPGLLALPARAQSGPGYATLKDRAPVDAPAG
                     QIEVIEFFSYGCSHCKNFEPVFEAWKKAAPKDVTVRREHVGFQSSFEPLQRIYYALDA
                     LGLTEQPVHMKVFQALQTERKRLDKPDVLLPWIAEQGVDRAKFEAAYKSFGIANKLRR
                     AVQLQEAYHVEGTPALGIAGRYYTDGSMAGGFERMIQIANNLIAQERKK"
     gene            complement(7773..8522)
                     /locus_tag="PK242_08590"
     CDS             complement(7773..8522)
                     /locus_tag="PK242_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOZ94116.1"
                     /translation="MAPTMMICKQRGGTFLGLLLGVLVGLAAALAVAIYVAKVPVPLV
                     SKNATRTNGQDAAEAEKNKDWDPNAALRGKNGTRPAGPGPAASGSVVPAPAPAPIPAP
                     TEVARPAPDSKPADARAPARGADKTAEPAPPAVTARPAPARPSSDPLGDLAAARSGAA
                     PAAPAAAAAADPFAYFVQAGAFRTPEDADAQRARLSLMGVEARVSEREQAGRTVYRVR
                     VGPFQNRDAADRVKQRLDGEGFDAALVRVQK"
     gene            complement(8527..>9043)
                     /locus_tag="PK242_08595"
     CDS             complement(8527..>9043)
                     /locus_tag="PK242_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HOZ94117.1"
                     /translation="SKRAGSYVTLRDLIDWTSADAVRFFLLSRKPDTEYTFDVDLAVQ
                     QNNDNPVYYVQYAHARICSVLQAWGGDAATLADADLTPLDTPAAQALMLQLARYPDML
                     SAAAQDFAPHDVTFYLRELAAAYHSYYDAERILVDDPAVKTARLALVAATAQVLHNGL
                     TVLGVTAPTRM"
BASE COUNT         1192 a         3123 c         3334 g         1394 t
ORIGIN      
        1 aaagttcatg gtgtctcctg tgggttctgg gtgaaaggcg tgacggtcgc cggcggcagg
       61 ccgtgcgctg cgttgtccgc gcccaggcgc ggcgcggccg agcgtggcag ggggcgcgcg
      121 ccgtcgatgg aaaacggcag cgcggtgagc gcgaaatcct cgcccggcag cggcgccagg
      181 atgcccagcg cctgcacctg cggatgcgcc agcgcttcgg gtacgctgtg gatgggcgcg
      241 cagggcacgc ccgcggcctc gaagcgcgcg gcccattcgg cgcggggtcg cgcgcgcagc
      301 agcggctgca gctgcgccag cagctcggcc ttgtgcgcca gccgcgcgcg attgctggca
      361 aaacgctcgt cggccagcca gtcgggccgg cccagctcgg tcgccagctt ggcaaacagg
      421 cggtcgttgc cgcagcagat caaaagcggt gcgtcctggg tgtcgaacgc ctcgtagggc
      481 acgaagccgg ggtggcccga acggtcgcgc agcggcaggc gaccttcgtt gacgtaggca
      541 tcggccttct ggccgttcca gaccagggcg gtttcgagca gcgaggtctg caagatgccg
      601 ccgcgcccgg tctgctggcg ccgctgcagc aaggccagcg cgccgatcac ggcccacatg
      661 cccgagccct ggtcgcacat cgacgcgccc gagcgcatgg gcgggtcatc cgggccgccg
      721 ttgatgctgg acaggccgct gaaggcctgc acgaggggtt cgtagcccgg ctgcatggcc
      781 atcgggccgg tgctgccgaa ggccgagatc tcgcagtaga tcaggcgcgg gtggcgtgcg
      841 cacaggctgg ccccgtcaat gcccagcttg gccggcacgc cggggcgcag gttgtggatg
      901 aagatgtcgg cctcgcccac caggcgctcg aacgcggcca gcccgtcggc gcttttcagg
      961 tccagcgcca ccgactgttt gccgcgattg aagatgtgga agttgagcgc gtcgccatgc
     1021 cggaacgccg gtcccatggc gcgcgcgtcg tcgccgcctt cgacgcgctc gaccttgacc
     1081 acttcggcgc ccaggtcggc gaagatcgcg ccggcaaacg gcccggccag cacctggccc
     1141 agctccagca cgcgcacacc cgccagcacg cccatcgcgg tcgcattact cacgcttgtc
     1201 tcctttggat gaaaggcagt ctaggctcgc acgtctgata tgggaatagc tatatttcta
     1261 tatctgtgat aacgattggg gatcaatgcc atgcgccttg acctgagcct ccggcagctc
     1321 gaagccttcg tacaggtggc cgacagcggc agctttcggc gcgcggcggc gcggctgggg
     1381 caggcgcaac cggtgctcag ccgcctgatt ggccaggccg agcagacgct gggcgcgcgc
     1441 ctgttcgacc gcgacacgcg ccgggtcgag atcacgccgg ccgggcgcga gctgctgccg
     1501 ctggcccagc gcctgctgcg cgacttcgac gacacgctgg gcgactttgg cgccttcatg
     1561 gccgggcgca gcggccgcgt gtcgatggtg gtgctgccct cggtcagtgt ggcctgggtg
     1621 ccgcgcgcca tggccgcgtt tgctcgcacg catccgcagg tgaactttgc cctcgccgag
     1681 gcgccggccg acaccctgct ggcgctgctg gaggccgggc gcgccgattt cggcatcagc
     1741 gtgcggccgg cgccgcagca gcgcctgcac taccgtcacc tgcaggacga cccgttcgtg
     1801 ctggtgtgcc ggcgcgacga tccactggcg gcgcgcgcgg ccgtgccctg gtcggtgttc
     1861 ggcatgcgcg attgcatcat gagcgcggcg cacagcagca tccgccaggt gacggacgcg
     1921 gtgtttctgc gccagcgcac gccgcccagc ccggtgctgg aataccccag cgtggcggcg
     1981 gccggcgcgc tggtcgcggc cggcctgggc atcacggcgc tgccggtgct ggcgcttcag
     2041 ctggtggaca tgcgcgagct ggtggcggtg ccgctggtgc gcccggccat ggcgcgcccg
     2101 atcggcatcg tcacgcgcat cgggcgttcg ctgtcgcctg ccactcaggc gttcatggcg
     2161 tcgctgcagg cggcgccggc gggggcgccc gacgcgccgt gaggtcaaag cgcagcagcc
     2221 ggcactcgat cgggccgttc cacagcggca cgcggcgcga ttccttcagg cgcaggcggc
     2281 cgggcagctt gctgtcgggc gtcagcagcc aggcgctcca gccggcgtag tggctcttcc
     2341 agtgcgcggc caggcgggcg aagaaatcgt cgccggcgtt gacatcgtcg gcaggcgccg
     2401 cgccccccgc gcgctggcgc gccagcgtgc cggactgaaa ggcctcgcgc ccgccgcgcg
     2461 cgccggccac gccggcggcg gcgatgcgct cgccataggg cggattgagc agcagcacgc
     2521 ccggctccgc gcagggcggc aggcgctgca gtgcgtcgcc gccgcgaagc tgcacggcgg
     2581 ccgccacgcc ggcgcgccgg gcgttgcgct gggcgaagtc gaccatgcgg tgggcgacgt
     2641 cgctgccgaa gatgcgcacc gccggggttt gcgcatcatt ttggtctccg gcgcttgtgg
     2701 agcaagcgcc agcagcttcg gttttgatag cgttccagcc tgccggatcg tgcggccgca
     2761 gctgctcgaa ggcaaagcgc cgctggccgc cggcgggcag gccgagcagc agttgcgccg
     2821 cctcgatgac gatggtgccc gagccgcagc aggggtcgta cagcggcagc ggcgacacgc
     2881 tgcgcgtggc cggctgccac cagccgcagg ccgccagcat ggcggcggcc agggtttcct
     2941 tcagcggcgc gtcgcccttg tcctggcgcc agccgcgctt gaacagcggc tcgccgctgg
     3001 tgtcgatgta cagcgtggcg tcctgcgggc ccaggtgcgc ctgcacgcgc acgtcgggct
     3061 gctgggtgtc gatgctgggg cgcacgccgc cggcctgctg gcgaaagcgg tcggccaccg
     3121 cgtccttgat gcgcagggtg gcgaagttca ggcttttcag cgggctggcc tgcgccgtca
     3181 gatcgacgcg aaagcgctgg cgcggggtga accactgttc ccacggcacg ccggcggcca
     3241 gcgcgtacag gtcctgctcg ctctggtagg ggccgtgggc cagctcgatc agcacgcgct
     3301 gggccaggcg actgtggagg ttcaggcgct gcatcagcgc ccagtcgccg cgcacgcgca
     3361 cgccgccgcg caggctcaag aggtcttcgc ccaccaggcc tgagaggcca tgcacctcgt
     3421 cggccagaaa atcctccacg ccgccggcgc agggcagaaa cagggtcagc gcgttcatgg
     3481 cgtcgctgcc gcggcgcagg ccagcagctt gccctgaatc ggcgccggga tgggggtgac
     3541 gcggcgcgag acgggatcga tgaacacgat gccggtcttg ccgcgcgcca cctcgcggcc
     3601 ctggctgact tcggtcatgc gaaacaccag atcgaagccg tagcggttga agtcggccgg
     3661 ctgcatctgc acgcgcagcg tttcgccgtg gaaggcctcg gacttgtact gcgccagcat
     3721 gtcgcccacc acgatggcgg cgccgtccac atggccttcg cggtagccca gggcgtggaa
     3781 aaagcgcagc cgcgcctcgg acaccagcgc cagcagctgg gcgttgtcca ggtgcccgcc
     3841 ctggttgacg tggctgatgt agatctgcag ctcggtggca aagccgaact gcgcgggaag
     3901 gtcgaagacg atgcgggcca tggtgaaacg ggggctcagt ggctcttgcg caaagaggcg
     3961 gtgggcaggc gcagggcctc gcggtacttg gccacggtgc ggcgcgcgca gtcgatgccc
     4021 tgggccttca actgctcggc gatctgggcg tccgacagcg ggcgcgcgcg gtcttcggct
     4081 tcgatcagct gccggatcag tgcgcgcacc gcggtgctgc tggcgtggct gcccgattcg
     4141 gtgcccagcg ccgagccaaa gaagtacttc agctcgaagg tgccgcgcgg tgtggccatg
     4201 tacttggcgg tggtgacgcg cgagatggtc gattcgtgca cccccagctc gtcggccagc
     4261 tcgcgctgga tcagcgggcg catggccagc tcgccgtgga tgaagaagtc gcgctggcgc
     4321 agcacgatgg cgttggccac gcgcaggatg gtgtcgaagc gctgctggat gttgcggatg
     4381 aaccagcgcg cttcctgcag gcgttcgctg agcgccgggt gcttgtcgct gccacgcagg
     4441 gcgctggcgt actgctcgtg cacgcgcagc cgcggcacca cgtcggggtt gagcgccacg
     4501 cgaaagcgcg gccgctcggc cgtgccgacg gcgctgacga tgacgtcggg cacgacgatc
     4561 tggcgctcga cgtcggcaaa gcggcgcccc ggcttgggat cgagccgccc gatcagggcc
     4621 atggcggcgc gcaccgcgtc ctcgctctcg ccgacgagct gcgccagctt gcggctgtcg
     4681 cgccgcgcca gcagctcgac ggcgtcgtgg cgggcgcaga tgcgcagcgc ggtggcgatg
     4741 cgggcctgct cctgcggcgc cagcgcgcgt tcggcctggc ggctgcgcaa ctgcagcgtc
     4801 agacattcgg ccaggtggcg cgcgcccacg ccggccggct ccagcgactg cagcagcttg
     4861 agcgccagcg tcaggcggtg caccagctcc tgcatctgct ccagctcgtc gtcggcgtcg
     4921 gtcagcgtgg ccgccagctg cgccagcgaa tcctccaggt agccgtcgtc gttcagcgag
     4981 ccgatcagaa agcgcagcgc cgccgcgtcc tcgggcgcca ggcgcagcgt cagcgcctgg
     5041 cgcagcaggt gctcggccag cgattcctgg ccgccgacgc gctcgcccgg gtcggtgtcg
     5101 tcgtcgcccg cctcgcgctg gcgcgccggt gcctcgccgc cccattcggc gtcgtccggg
     5161 gcgatgtcca cgctgccgtc gccgtcccag tcgtccgggg tcaggggttc ggcatcaaaa
     5221 tcggcttcgg cgcttgtgga atctgcgcca gcagctcctg aatcaatagc gttttcgtcg
     5281 gtgcgttcgc cccggtcggc cggcgtcggc gcggctgcgt cgccggcctc gtcgtcgcgc
     5341 tccagaaacg ggttgtcggc cagcatctgc tcgacttcgc cgctcagctc cagcgtcgac
     5401 agttgcagca accggatcga ctgctgcagc tggggcgtca gcgccagctg ctgcgccacg
     5461 cgcagcgaaa gcccgggttt cacagccgga aatgctcgcc cagatagacg cgccgcacct
     5521 cggggttggc cacgatgtcg gccggcgtgc cggtggccag cacgcgacct tcgctgatga
     5581 tgacggcgtg gtcgcagatg cccagggttt cgcgcacgtt gtggtcggtg atcagcacac
     5641 cgatgccctg gctcttgagg aaggcgatga tgcgctggat ctcgatcacc gcgatcgggt
     5701 cgatgccggc gaaaggctcg tccagcagga tgaagcgcgg ctgcgtcgcc agcgcgcgcg
     5761 cgatctccac ccgccggcgt tcgccgcccg acagcgccgg cgccggcgaa tcgcgcaacg
     5821 gctcgacatg cagctgctgc agcagctcgt tcaggcgctg ctcgatggcg tcgcgcccca
     5881 ggggccgacc ctgggcgtcc acctgcagct ccagcaccgc gcgcacgttt tcggccaccg
     5941 tcagcttgcg gaagatcgag gcctcctgcg gcaggtagga caggcccagg cgcgagcgcc
     6001 ggtggatcgg catgtgctcg atcggctggt cgtcgatggc gatgctgccg gcgtcggcac
     6061 gcaccaggcc gacgatcatg tagaaggagg tggtcttgcc ggcgccgttg gggcccagca
     6121 ggccgaccac ctcgccgttg accacgtcca gcgacacgtc gtgcaccacc cggcgtgcgc
     6181 cgtagttctt gcgcaggccg cgcacctgca gccggctctg gccgtgcgcg cgcggcgcgt
     6241 cggcggcggc gggggcgtca aagcgcgacg ccagcgcctc cagcgccggt tcgtgcacgg
     6301 gcgtgttcac tgtccgcttc cttcgggcag cgcagcggcc gggcgcagct gcagcgccgg
     6361 tgccgaagcc gcggacgcgg cgctggcggc ctggcgcggc gccagcgtgg cgcgcacgcg
     6421 cccgcccggc gccgccgagg gtgcgccggc cgcgggtgcg ccgtccaccg tgtacacctc
     6481 ggtgcggttg ttgtagacga tcaggctgcc cagcacctgg tccagctggg tggcgccctc
     6541 caggcgccgc atctcggcgc ggcggatcag gcgcacggtg tcggtcttgc cgtcgtattc
     6601 gatggtttcg ccttcgcctt cgatgaactc gttcacgccc tcgcgcttct gacggaagaa
     6661 ggcgcgcttg cccggctcgc ccgacagggt gccgaactgg ttgccggcgc tgtcctggcg
     6721 cacttccagg cgggcgccgc gcatcacgat ggtgcccttg gtcaccagca ccttgccggt
     6781 gaaggtggac tgctgcttct gctcctcgta gcgcagcgcg tcggcctcga tgttcatggg
     6841 cttgtcgcgg tcggcgcgct cggcccaggc cggcgcggcc aggccgagca gcagggtggc
     6901 gcacagcagg cgcaggctgg gagcgaacgg cggtacggac gacttcatcg cgaaggcacg
     6961 gtttttgctt tcagtatgaa acattctaag cggccgccat gcgtgccgca gcggtgcggc
     7021 acatcaaaaa agcccgccaa ggcgggctgc tgcgtgcgtc gcccgagcgg cgcgtcactt
     7081 cttgcgttcc tgggcgatca ggttgttggc gatctggatc atgcgctcga agccgccggc
     7141 catcgagccg tcggtgtagt agcgcccggc aatgcccagc gccggcgtgc cctcgacgtg
     7201 gtaggcctcc tgcaactgca ccgcgcggcg cagcttgttg gcgatgccga acgatttgta
     7261 ggcggcctcg aacttggccc ggtccacgcc ctgctcggcg atccagggca gcagcacgtc
     7321 cggcttgtcc aggcgcttgc gctcggtctg cagggcctgg aacaccttca tgtgcaccgg
     7381 ctgctcggtc aggcccaggg cgtcgagcgc gtaatagatg cgctgcagcg gctcgaagct
     7441 gctctggaag ccgacatgtt cgcgccgcac cgtcacgtcc ttgggggcgg ccttcttcca
     7501 ggcctcgaac accggctcga agttcttgca gtgcgagcag ccgtaggaga agaattcgat
     7561 cacctcgatc tgcccggccg gcgcgtcgac cggggcgcgg tccttgagcg tggcgtagcc
     7621 ggggcccgac tgggcgcgcg ccggcagtgc cagcaggccg ggcagggcca gcgcgccagc
     7681 gagggtgaat tcacgacgat tcatggaaac ggactccttg tctatggact gggttcagag
     7741 cgtcgccgtc gggcggagtt cccgcgccgc ggtcatttct gcacccgcac cagcgcggcg
     7801 tcgaagccct cgccgtccag ccgctgcttg acgcggtcgg ccgcatcgcg gttctggaac
     7861 gggcccacgc gcacccggta gacggtgcga ccggcctgtt cgcgctcgct cacgcgcgct
     7921 tccacgccca tcagcgacag gcgggcgcgc tgggcatcgg cgtcttccgg ggtgcggaag
     7981 gcgcccgcct gcacgaagta ggcgaagggg tcggcggcgg ccgccgcggc aggcgccgcc
     8041 ggcgcagcgc cgctgcgcgc cgcggccaga tcgcccagcg gatcgctgga cgggcgggcc
     8101 ggcgccggcc gcgcggtcac cgcggggggt gccggctcgg ccgtcttgtc ggcgccgcgc
     8161 gccggggcgc gcgcgtcggc cggcttgctg tcgggggccg ggcgggccac ttcggtcggt
     8221 gccgggatgg gcgccggcgc cggtgccggc accacgctgc cgctggcggc ggggcccggg
     8281 ccggccgggc gggtgccgtt tttgccacgc agcgcggcgt tcgggtccca gtccttgttc
     8341 ttttcggcct cggccgcgtc ctggccgttg gtgcgggtgg cgttcttgct gaccagcggc
     8401 accggcacct tggccacgta gatggccacc gccagcgccg ccgccaggcc caccagcacc
     8461 ccgagcagca gacccaggaa ggtgccgccg cgttgcttgc agatcatcat cgttggagcc
     8521 atgccatcac atccgggtgg gcgccgtgac gcccagcacc gtcaggccat tgtgcaacac
     8581 ctgggcggtg gcggcgacca gggccaggcg cgcggtcttc accgcggggt cgtccaccag
     8641 gatgcgctcg gcgtcgtagt agctgtggta ggcggcggcc agctcgcgca ggtagaaagt
     8701 gacgtcgtgc ggcgcgaagt cctgcgcggc ggcgctcagc atgtcggggt agcgggccag
     8761 ctgcagcatc agcgcctgcg ccgccggcgt gtccagcggc gtcaggtcgg cgtcggccag
     8821 cgtggccgcg tcgccgcccc aggcctgcag caccgagcag atgcgcgcgt gggcgtactg
     8881 cacgtagtag accgggttgt cgttgttctg ctgtacggcc agatcgacgt cgaaggtgta
     8941 ttcggtgtcg ggcttgcgcg acagcaggaa aaagcgcacc gcgtcggcgc tggtccagtc
     9001 gatcaggtcg cgcagcgtga cgtagctgcc ggcgcgcttg ctg
//
LOCUS       DAOOOG010000103         8918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_9158_length_8918_cov_30.051450, whole genome shotgun sequence.
ACCESSION   DAOOOG010000103 DAOOOG010000000
VERSION     DAOOOG010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8918
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9158_length_8918_cov_30.051450"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1066)
                     /gene="fba"
                     /locus_tag="PK242_08600"
     CDS             complement(2..1066)
                     /gene="fba"
                     /locus_tag="PK242_08600"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060402.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HOZ94118.1"
                     /translation="MPLVSMRELLDHAAEHNYGIPAFNVNNLEQVQAVMAAADEVGAP
                     VILQASAGARKYAGEHFIKYLIQAAAEAYPHVPMVMHQDHGTSPAVCAGALKLGFGSV
                     MMDGSLKEDGKTPADFDYNVRVTQEVVAMAHQIGATVEGELGCLGSLETGEAGEEDGI
                     GAAGKLDHSQLLTDPEEAAQFVKATQLDALAIAIGTSHGAYKFTRPPTGDVLAISRVK
                     EIHARIPNTHLVMHGSSSVPQELLAIINQFGGKMKQTWGVPVKEIQEAIKHGVRKINI
                     DTDIRMAMTGAVRKFQVENPDKFDMREWMKPARAAAQAICKQRYLEFGCEGQGAKVKG
                     LPLSVMAQKYAQGALAQQVR"
     gene            complement(1347..2780)
                     /gene="pyk"
                     /locus_tag="PK242_08605"
     CDS             complement(1347..2780)
                     /gene="pyk"
                     /locus_tag="PK242_08605"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOZ94119.1"
                     /translation="MTYRATKIVATLGPASSDPALLEQMIRAGVNVVRLNFSHGKAED
                     HVERARLVRQASERAGREVAIMADLQGPKIRVGKFAEGKVLLAPGAPFVLDAARTEPG
                     DLQGVGLDYKQLPRDVRPGDTLLLNDGLIVLTVEAVRGEAVHTRVKLGGELSNNKGIN
                     KQGGGLTAPALTAKDMEDIKTAMALRADYVAVSFPKNATDMEMARQLCNVAGAEWGHK
                     PQLIAKIERAEAIPRLAEILAASDGIMVARGDLAVEVGNAAVPALQKKMITMARAADK
                     LVITATQMMESMIANPVPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPLETVQEMAA
                     ICMAAEEHDPAELDADFSGATFQRIDQSIAMGALFTAYHLGAKAIVALTDSGSTALWM
                     SRHRVRIPIYALTPKVGTQRKLALYRNVRPLLMDTSALRDEALAQAEAYLKQQGVMAS
                     GDVYAITCGEPMGAPGGTNMLKICRAG"
     gene            complement(2894..4090)
                     /locus_tag="PK242_08610"
     CDS             complement(2894..4090)
                     /locus_tag="PK242_08610"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOZ94120.1"
                     /translation="MNVIRFSDLCAQGAVQGQRVFIRADLNVPQDDGGAITDDTRIRA
                     SVPAIRMALQAGAAVMVTSHLGRPTEGECKPADSLAPVARRLGELLGREVPLVQHWVD
                     GVTVAPGELVLLENCRVNKGEKKNDEALSRKMAALCDIYVNDAFGTAHRAEATTYGIA
                     QYAPVACAGPLLAAEIDALQQALAHPRRPLVAIVAGSKVSTKLTILQSLASKVDQLIV
                     GGGIANTFMLASGLPIGKSLAEADLVGEARAVIEAMRARGAEVPIPTDVVTATEFKAD
                     AVATVKPAQAVAADDLILDIGPDTAERLAAQLQAAGTIVWNGPVGVFEMAPFENGTRR
                     IARAIADSPAFSIAGGGDTLAAIAKYGIDKDVGYISTGGGAFLEVLEGKQLPAFEILE
                     KRAASA"
     gene            complement(4386..4733)
                     /locus_tag="PK242_08615"
     CDS             complement(4386..4733)
                     /locus_tag="PK242_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOZ94121.1"
                     /translation="MHELDGWTLLAILGMTAISVITRGFFFISEREWTLPEWAQRGLQ
                     YAPIAALTAVIAPDILLTEGVVGAVLRDARTWGALAAAGFYFWRRRGSYVMPAAIAVG
                     MAVFLPLRVGLGW"
     gene            complement(4726..5415)
                     /locus_tag="PK242_08620"
     CDS             complement(4726..5415)
                     /locus_tag="PK242_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOZ94122.1"
                     /translation="MAGVSIGLTAWAFMTGVAMVKSGMSITESVLMSLLVFAGSAQLA
                     AIPLIAADAPLWVVLAAAFCVNLRFVVFSLHLRPYFMHLPRFKRLYLGYFTGDVTYVL
                     FTRRFPRPPETEGQRRAQGSYLWGGNVTNWVFWQSLSMLGIFLGAALPSRWGLEFAGT
                     LALLAVACSMVTTRLRALSAITAAAAAVVLFGLPYRLNIVAAIAVAVAISLYAEPRPP
                     AAGDDAGARDA"
     gene            complement(5544..6671)
                     /locus_tag="PK242_08625"
     CDS             complement(5544..6671)
                     /locus_tag="PK242_08625"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOZ94123.1"
                     /translation="MRVIFAGTPEFARVALQALQAAGFEVPLVLTQPDRPAGRGMKLQ
                     ASPVKQLALQHGIAVAQPRSLRLDGKYPEDAAAARAALRAARADAMVVAAYGLILPQW
                     VLDDMSTAGPPQGASAPSGGSATGAAVERGGRDPSTAGPPQGASAPSAGSATGQAFGC
                     LNIHASLLPRWRGAAPIHRAIEAGDAQTGVTIMQMDAGLDTGDMLLAQALPIGPRATT
                     ASLHDELAALGGQLIVQALQQAAQGRLRPTPQPAEGVCYAHKIDKHEAAIDWHQPAAQ
                     IERRIRAFDPFPGASSQLGDTVVKLWNSEIDSCSRPSDARCGQILAVDEGGIAVACAD
                     AVLRLTELQRPGGKRLPAREFLRGFALAPGQCLGEGTGGAP"
     gene            complement(6741..7226)
                     /locus_tag="PK242_08630"
     CDS             complement(6741..7226)
                     /locus_tag="PK242_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94124.1"
                     /translation="MPAALGALLLGACAALLNPDRVAPGTPAAQVLQRLGPPTARYPA
                     TAQAGERWQYSQQPAGRQVFNLDLDAQGRVVRVEQALSEARFGQRIQPDRWTREDVLR
                     EYGRPAQVTAVHRFDGDIWVWRYLDGPTWRLLFIDIDRAGVVRGWSVGDENLPDPPEP
                     R"
     gene            complement(7283..7813)
                     /gene="def"
                     /locus_tag="PK242_08635"
     CDS             complement(7283..7813)
                     /gene="def"
                     /locus_tag="PK242_08635"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOZ94125.1"
                     /translation="MAQLPILRYPDPRLHKVARPVQVVDARVQALIDDMFETMYAAKG
                     IGLAATQVDVHERLIVIDVSEERNQRLVLINPELLWASEQRVRGEEGCLSVPGIYDGV
                     ERAERVRVRALDAAGEAREIEAEGLLAVCIQHEMDHLLGKVFVEYLSPLKRGRIKTKL
                     LKAERQEKADQARVPA"
     gene            7957..>8918
                     /locus_tag="PK242_08640"
     CDS             7957..>8918
                     /locus_tag="PK242_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOZ94126.1"
                     /translation="MKLLFPGATRALVAPLGLLTAALLGAPPAAAQAASRLPVTPMQR
                     ATAQQVAAQGVPLSALAPDAPDQYTVRRGDTLWRISSLFLKSPWRWPELWGMNLQDIR
                     NPHLIYPGQQLYLERLGDRAFLRARRGADGEVGDTVRVSPRNRIEMLGTGPLPTLEPH
                     LIEPFLTEPLVVDDDSFQRAPRIVALANQSRVLVAKGDRAYVRGPADAPLLKQAGDPT
                     EFRVFRNAKPLKDPVTGETLGYEGHYVGQATLVRGESEAEEDAGAAPAVRPTRWPANG
                     EVQSEPRRAAAPQPRALPVPATVDVISSKEEMRAGDRLLPEPPR"
BASE COUNT         1275 a         3150 c         3109 g         1384 t
ORIGIN      
        1 ctcagcgcac ctgctgcgcc agcgcgccct gggcgtattt ctgcgccatc acggacagcg
       61 gcaggccctt gaccttggca ccctggccct cgcagccgaa ttccagatag cgctgcttgc
      121 agatggcctg ggcggcggcg cgggcgggct tcatccactc gcgcatgtcg aacttgtcgg
      181 ggttttcgac ctggaacttg cgcaccgcgc cggtcatggc catgcggatg tcggtgtcga
      241 tgttgatctt gcgcacgccg tgcttgatgg cctcttgaat ctccttgacc ggcacgcccc
      301 aggtctgctt catcttgccg ccgaactggt tgatgatggc cagcagctcc tgcggcacgg
      361 acgatgaccc gtgcatcacc agatgggtgt tggggatgcg ggcgtggatt tccttgacgc
      421 gcgagatcgc cagcacgtcg cccgtgggcg ggcgggtgaa cttgtaggcg ccgtggctgg
      481 tgccaatcgc aatggccagg gcgtcgagct gcgtggcctt gacgaactgg gcggcttctt
      541 cggggtcggt cagcagctgg ctgtggtcga gcttgccggc ggcgccgatg ccgtcttcct
      601 cgccggcctc gccggtctcc aggctgccca ggcagccgag ctcgccctcc acggtggcgc
      661 cgatctggtg cgccatggcc accacttcct gcgtcacgcg cacgttgtag tcgaagtcgg
      721 ccggggtctt gccgtcttcc ttcaggctgc cgtccatcat caccgaacca aagcccagtt
      781 tgagcgcgcc ggcgcacacg gccgggctgg tgccgtggtc ctggtgcatc accatgggca
      841 cgtgcggata ggcttcggcg gcggcctgga tcaggtattt gatgaagtgc tcgccagcgt
      901 atttgcgcgc gccggcgctg gcctgcagga tgacgggggc gccgacctca tcggcggcgg
      961 ccatcacggc ctgcacctgc tccaggttgt tgacgttgaa ggcggggatg ccgtagttgt
     1021 gctcggccgc gtggtcgagc agttcgcgca tggaaacgag gggcatggtc agggttctcc
     1081 gaaggcaggg cggtgtggcg gcggccgacg cccgggtgtg cgacgagttt acctgctgcc
     1141 tctgacccta gaaacggcag ttgaccgcgt gtcaacttcg ggcagaccgc atcgatgcgg
     1201 ggaccggcgg cttcgagggc cttcacctcc tgggtgcgca cgccacgcac gcacccggac
     1261 cgcgctcggc gcgcacgcgc ggggcgtgca aggccgatgg accccaaggg ggcgcgaccg
     1321 gtctcatggg cccgccgctg cgccgatcag ccggcgcggc agatcttgag catgttggtg
     1381 ccgccggggg cgcccatggg ttcaccgcag gtgatggcgt agacgtcgcc gctggccatc
     1441 acgccctgct gcttgaggta ggcctcggcc tgcgccagcg cctcgtcgcg cagggcgctg
     1501 gtgtccatca gcagcgggcg cacgttgcgg tacagcgcca gcttgcgctg ggtgccgacc
     1561 ttgggcgtga gcgcgtagat ggggatgcgc acgcggtggc ggctcatcca cagcgcggtg
     1621 gagccggaat cggtcagcgc cacgatggcc ttggcgccca ggtggtaggc ggtgaacagc
     1681 gcccccatgg cgatcgactg gtcgatgcgc tggaaggtgg cgccactgaa gtcggcgtcc
     1741 agttcggccg ggtcgtgctc ctcggccgcc atgcagatgg cggccatctc ctgcaccgtc
     1801 tccagcgggt acttgccggc cgccgtctcg gcgctcagca tcacggcgtc ggtgccgtcg
     1861 agcacggcgt tggcgacgtc gctgacctcg gcgcgcgtgg gcaccgggtt ggcgatcatg
     1921 ctctccatca tctgggtggc ggtgatcacc agcttgtcgg ccgcgcgcgc catggtgatc
     1981 atcttctttt gcagcgccgg cacggcggcg ttgccgactt ccaccgccag gtcgccgcgc
     2041 gccaccatga tgccgtcgct ggcggccagg atctcggcca gccgcgggat ggcctcggcg
     2101 cgctcgatct tggcgatgag ctgcggcttg tggccccatt cggcgccggc cacgttgcac
     2161 agctggcgcg ccatttccat gtcggtggcg ttcttgggaa agctcacggc cacgtagtcg
     2221 gcgcgcaggg ccatggcggt cttgatgtcc tccatgtcct tggccgtcag cgccggcgcc
     2281 gtcaggccac caccctgctt gttgatgccc ttgttgttgg acagctcgcc acccagcttg
     2341 acgcgggtgt gcaccgcttc gccgcgcacc gcctccaccg tcagcacgat caggccgtcg
     2401 ttgagcagca gggtgtcgcc cggccgcacg tcgcgcggca attgcttgta gtccagcccg
     2461 acgccctgca ggtcgcccgg ctcggtgcgc gcggcgtcca gcacgaacgg cgcgcccggc
     2521 gccagcagca ccttgccctc ggcaaacttg ccgacgcgga tcttcggccc ctgcaggtcg
     2581 gccatgatgg ccacctcgcg gccggcgcgc tcgctggcct ggcgcaccag gcgggcgcgt
     2641 tccacatggt cctcggcctt gccgtggctg aagttcagcc gcaccacgtt gacgccggca
     2701 cggatcatct gctccagcag cgccgggtcg ctggaggcag ggccgagggt ggcgacgatc
     2761 ttggtggcgc ggtaggtcat ggctgggctg ggtgcaggcg gaaatcgcag gccggattct
     2821 gccagcgccg catgacggcg ccgtgatcgg gcgccagatt gctatgactt ttatagctgt
     2881 tggcgcttgc ccatcaagcg ctggcggccc ttttttccag aatctcgaag gcgggcagct
     2941 gcttgccctc cagcacctcc agaaaggcgc caccgccggt ggagatgtag cccacgtcct
     3001 tgtcgatgcc gtacttggcg atcgccgcca gggtgtcgcc gccgccggcg atgctgaagg
     3061 cgggcgaatc ggcgatggcg cgcgcgatgc ggcgcgtgcc gttttcgaac ggcgccatct
     3121 cgaacacgcc caccgggccg ttccagacga tggtgccggc cgcctggagc tgcgccgcca
     3181 ggcgctcggc ggtgtcgggg ccgatgtcca ggatcaggtc gtccgccgcc accgcctgcg
     3241 ccggcttgac ggtggccacg gcgtcggcct tgaactcggt ggccgtcacc acgtcggtgg
     3301 ggatcggtac ctcggcgccg cgcgcccgca tggcctcgat cacggcgcgc gcctcaccca
     3361 ccaggtcggc ctcggccagc gacttgccga tcggcagccc gctggccagc atgaaggtgt
     3421 tggcgatgcc gccgcctacg atcaattgat cgactttgga cgccaaagac tgcaagatgg
     3481 tgagttttgt gctcacttta ctgccggcca cgatggccac caggggccgc ctggggtgcg
     3541 ccagggcctg ctgcagggcg tcgatctcgg ccgccagcag cgggccggcg caggccaccg
     3601 gggcgtactg ggcgatgccg taggtggtgg cttcggcgcg gtgcgcggta ccgaaggcat
     3661 cgttaacgta aatatcacaa agtgcggcca tcttgcgcga aagcgcttca tcgttctttt
     3721 tctcgccctt gttgacgcgg cagttctcga gcagcacgag ctcgcccggc gccaccgtca
     3781 cgccgtccac ccagtgctgc accaggggca cctcgcgccc cagcagttcg cccaggcggc
     3841 gcgccaccgg cgccagcgag tcggcgggct tgcattcgcc ctcggtcggg cgacccaggt
     3901 gcgaggtgac catcaccgcc gcgccggctt gcagcgccat gcggatggcc ggcacgctgg
     3961 cgcggatgcg ggtgtcgtcg gtgatggcgc cgccgtcgtc ctgcggcacg ttcaggtcgg
     4021 cgcggatgaa gacgcgttgg ccctgcacgg caccttgcgc gcacaggtcg gagaagcgga
     4081 tgacgttcat gacaggcaga tcgggtaagt gaaaaaacac aggacccgcc ggcggcggga
     4141 gggcgatgcg gcagcgccgc cattgtagga gcgcgcgccc acaccaccct gaccggtccc
     4201 gggcttcgga ttcgctacac gatcaatagc tgctggcgct tgactgaagg gccacgtcgc
     4261 ccggtttgcc catgatcctc ctgtttcgga aacttggaaa tccagaccca aaaggcgcca
     4321 tcaaggggaa tcggatcgtc cgtcccgcag ccgccaggcc cggcccgacc gggccgtgcg
     4381 ccgcttcacc agcccagccc gacccgcagc ggcaggaaca ccgccatgcc gaccgcgatg
     4441 gcggccggca tcacgtaact gccgcgccga cgccagaagt agaaaccggc cgccgccagc
     4501 gcgccccagg tgcgggcgtc gcgcagcacc gcgccgacga cgccctcggt cagcaggatg
     4561 tcgggcgcga tcaccgcggt cagcgcggcg atgggcgcgt actgcaggcc acgctgcgcc
     4621 cattcgggca gcgtccattc gcgctcgctg atgaagaaga agccgcgcgt gatgaccgaa
     4681 atggccgtca tgcccaggat ggccagcagc gtccagccgt ccagttcatg catcgcgcgc
     4741 ccctgcgtcg tcgccggccg ccggcgggcg tggctcggcg tacaggctga tggccaccgc
     4801 caccgcgatc gccgccacga tgttgagccg gtacggcagc ccgaacagca ccaccgccgc
     4861 cgccgccgcg gtgatggccg acagcgcgcg caggcgcgtg gtcaccatcg aacaggccac
     4921 cgccagcagc gccagcgtgc cggcgaactc cagcccccag cgcgacggca gcgcggcgcc
     4981 gagaaagatg cccagcatgc tgagcgactg ccagaacacc cagttggtga cgttgccgcc
     5041 ccacagatag ctgccctggg cgcgccgctg accctcggtt tcgggcgggc gcggaaagcg
     5101 ccgggtgaac agcacatagg tcacgtcgcc cgtgaagtag cccaggtaca agcgtttgaa
     5161 tctaggcaga tgcatgaaat atggccgcag atgaaggctg aagactacaa atcgcagatt
     5221 cacgcagaac gccgccgcca gcaccaccca cagcggcgca tcggcggcaa tgagcggaat
     5281 ggcggccagc tgggcgctgc cggcaaacac cagcaaggac atcagcacgc tttcggtgat
     5341 cgacatgccg ctcttgacca tggccacgcc ggtcatgaag gcccaggccg tcaggccgat
     5401 ggaaacgccc gccatctcgc gcgcaccgac ccagaacagc gggtgccgcg ccgtgcgcgc
     5461 ccaggtatcg acgcgccgcg ccagccaggc gcccagccgg ccacgcgccg gcagctcgcc
     5521 cggatcgggc ggcagcggca tgatcatggc gcgccacccg tgccttcgcc caggcactgg
     5581 ccgggcgcca gcgcgaagcc gcgcaggaac tcgcgcgccg gcaggcgctt gccgcccggg
     5641 cgctgcagct cggtcaggcg cagcacggcg tccgcgcagg ccaccgcgat gccgccttcg
     5701 tctacggcaa gaatctggcc gcagcgcgca tcggacgggc gcgagcagct atcgatttcg
     5761 gagttccaca gcttgaccac ggtgtcgccc agctggctgc tggcgccggg gaaggggtcg
     5821 aaggcgcgga tgcgccgctc gatctgtgcc gccggctggt gccagtcgat ggcggcctcg
     5881 tgcttgtcga tcttgtgggc gtagcaaacg ccctcggccg gctgcggcgt cggccgcagg
     5941 cgaccctgcg cggcctgctg cagcgcctgc acgatcagct gcccgcccag cgccgccagt
     6001 tcgtcgtgca ggctggcggt ggtggcgcgc ggtccgatcg gcagcgcctg cgccagcagc
     6061 atgtcgccgg tgtccaggcc ggcgtccatc tgcatgatgg tgacgccggt ctgcgcgtcg
     6121 ccggcctcga tggcgcgatg gatcggcgcc gcgccgcgcc agcgcggcag cagcgaggca
     6181 tggatgttga ggcaaccgaa ggcctggccg gtggcgctgc ccgccgaggg ggcgctcgca
     6241 ccttggggcg gcccggcggt gctcgggtca cggcccccac gctccacggc tgcgcctgtt
     6301 gcgctgcccc ccgagggggc gctcgcacct tggggcggcc cggcggtgct catgtcatcc
     6361 agcacccact gcggcaggat cagcccgtag gcggccacca ccatcgcgtc ggcgcgggcg
     6421 gcgcgcaggg ccgcgcgggc ggcggcggcg tcttcggggt acttgccgtc caggcgcagg
     6481 ctgcgcggct gggcgacggc gatgccgtgc tgcagcgcca gctgcttgac cggcgaggcc
     6541 tgcagcttca tgccgcggcc ggccgggcgg tcgggctggg tcagcaccag cggcacctcg
     6601 aacccggccg cctgcagcgc ctgcagggcc acgcgggcaa actcgggggt gccggcaaag
     6661 atcacgcgca tgctcatgtt tcgatagctt ctggcgctgg cgggttggcg ctttggtggc
     6721 gattcaatgg cacggaccgg tcagcgcggc tcgggcgggt cgggcaggtt ttcgtcgccg
     6781 acggaccagc cgcgcaccac gccggcgcgg tcgatgtcga tgaacagcag gcgccaggtc
     6841 ggcccgtcca ggtagcgcca gacccagatg tcgccgtcga accggtgcac cgccgtcacc
     6901 tgggccggcc ggccgtattc gcgcagcacg tcctcgcgcg tccagcggtc gggctggatg
     6961 cgctggccaa accgcgcttc gctgagcgcc tgctcgacgc gcaccacgcg gccctgggcg
     7021 tccaggtcca gattgaacac ctggcggccg gccggttgct gcgaatactg ccagcgctcg
     7081 cccgcctggg ccgtggccgg gtagcgcgcc gtgggcggac ccaggcgctg cagcacctgc
     7141 gccgccgggg tgccgggcgc cacccgatcc gggttgagca gcgccgcaca agcccccagc
     7201 agcagcgccc caagcgccgc cggcaaggcc ttcaggacac gccggatgac gaatgcatca
     7261 gcctttgccg cgcagtgggc gctcacgccg gcacccgcgc ctggtcggct ttttcctgtc
     7321 gctcggcttt cagcagtttg gtcttgatgc ggccgcgctt gagcggcgac aggtattcga
     7381 cgaacacctt gccgagcagg tggtccatct catgctggat gcacaccgcc agcaggccct
     7441 cggcctcgat ctcgcgcgcc tcgccggccg cgtccagcgc gcgcacacgc acgcgctcgg
     7501 cgcgctcgac gccgtcgtag atgccgggca ccgacaggca gccctcctcg ccgcgcacgc
     7561 gctgctcgct ggcccacagc aactcggggt tgatcagcac caggcgctgg ttgcgctctt
     7621 ccgacacgtc gatcacgatc agccgctcgt gcacgtcgac ctgggtggcc gccaggccga
     7681 tgcccttggc ggcgtacatg gtctcgaaca tgtcgtcgat cagcgcctgc acgcgcgcat
     7741 cgaccacctg caccgggcga gccaccttgt gcaggcgcgg gtcggggtaa cggagaatgg
     7801 gaagctgggc catggaattc ttgcctcgga tgtggcgatt ttcgctactt ttcgcagttc
     7861 gcgctgcctc gggtgagtga tttcgttgcc ggatcaaggg cttgaatgca gaatgtccgg
     7921 tgacttgtca agttggcagc tgctttcagc aaaacaatga aacttctttt tcctggtgcc
     7981 acgcgcgcgc tggtcgcgcc cctcggactg ttgaccgcgg ccctgctcgg cgcgcccccc
     8041 gccgccgcgc aggcggcctc tcgcctgccg gtgacgccca tgcaacgcgc caccgcccag
     8101 caggtggcgg cgcaaggcgt gccgctgtcg gcgctggcgc ccgacgcgcc cgaccagtac
     8161 acggtgcgcc gcggcgacac cttgtggcgc atctccagcc tgttcctgaa gtcgccctgg
     8221 cgctggcccg agctgtgggg catgaacctg caggacatcc gcaacccgca cctgatctac
     8281 ccaggccagc agctgtacct ggagcgcctg ggcgaccgcg ccttcctgcg cgcgcgccgc
     8341 ggcgccgacg gcgaagtcgg tgacaccgtg cgggtgtcgc cgcgcaaccg catcgagatg
     8401 ctgggcaccg gcccgctgcc cacgctggag ccgcacctga tcgagccctt cctgaccgag
     8461 ccgctggtgg tcgacgacga cagcttccag cgcgcacccc gcatcgtcgc gctggccaac
     8521 cagagccgcg tgctggtggc caagggcgac cgcgcctacg tgcgcgggcc cgccgatgcc
     8581 cccctgctga aacaggcggg cgaccccacc gaattccgcg tgttccgcaa tgccaagccg
     8641 ctgaaagacc cggtgaccgg cgaaaccctg ggctacgaag gccactacgt cggtcaggcc
     8701 accctggtgc ggggcgaatc cgaagccgag gaagacgccg gcgcggcgcc cgccgtgcgc
     8761 cccacgcgct ggcccgccaa cggcgaagtg cagagcgaac cgcgccgcgc cgccgccccg
     8821 cagccgcgcg ccctgcccgt gcccgccacg gtcgacgtga tcagcagcaa ggaagaaatg
     8881 cgcgccggcg accgcctgct gcccgagccg ccgcgcga
//
LOCUS       DAOOOG010000104         8834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_9276_length_8834_cov_26.153548, whole genome shotgun sequence.
ACCESSION   DAOOOG010000104 DAOOOG010000000
VERSION     DAOOOG010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9276_length_8834_cov_26.153548"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PK242_08645"
     CDS             <1..1125
                     /locus_tag="PK242_08645"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOZ94127.1"
                     /translation="MTSEPSPAAPAAPPPVPAPVATEAGTAAPASPLAPAHAAAPRGA
                     GVSPGLWLAWLLAAAGLVVGLLLWQKLTGMQEQLARQSREAGTQSVEARTLARQAEEG
                     VRDMAGKVAALDARLAELAAYRAQLDELVHSVARARDENLAVDLEAALRLAQDQTQLT
                     GSVEPLLAALRTAERRLARSSDPRLAPVGRAVARDLERVKAASIPDAAGLLARLDQLL
                     RQVDDLPAANEVPHARSAPATTAPAAAAESAWGRLWQSVRDEAQGLLRVSRIERPEAS
                     MLSPEQVFFVRENLKLRLQGARLALLARQYEAARADLTAAAMSLGTWFDPASRRTQAA
                     ATLLQQVQASTRSAELPRVDDSLVALGQAVATAHTVPGTR"
     gene            1131..2411
                     /locus_tag="PK242_08650"
     CDS             1131..2411
                     /locus_tag="PK242_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HOZ94128.1"
                     /translation="MRAALWLMGLFAAAVALALFAGDNEGTVTVFWPPHRVDVSVNLA
                     LLLLLALFVLLYLALRALSAMVQLPRQARRWRTQQRERAMHATLIDAAAQLAAGRFLR
                     ARKAAESALTRVQALSDAAEAPPHAPSLRTLAHMTVAESAHALRDEAMRDQHLEHALD
                     VAGRAGAAPELREGVLLRAARWSLDQRDAGAALARLNELPAGATRRTAALRIKLKAAR
                     QSGHHAEALETARLLAKHGAFSPAAARSLLRGLAASLIDEVHDPAQLQAVWGSLDAGE
                     RAMPELALRAAQRMLALDGPRALVRQWLRPVWEQALAQPGSFGPALRARLVQVLEQAL
                     IATGEASDQQWLALVESAQQAHPRDPSLQYLAGMACRHRGLWGRAQLLLGQAAQSLPD
                     AELRRHAWRALAELAEQRGDEPAAAQAWKQAARS"
     gene            complement(2602..3624)
                     /locus_tag="PK242_08655"
     CDS             complement(2602..3624)
                     /locus_tag="PK242_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOZ94129.1"
                     /translation="MHAPLPDPRLRTATLASHGLIAASLLLVMWQGLLPGLLCVCVGF
                     FCTRWLSPRLALPARQADRQAPRLAATVVALLPLLLLALALPRTRGLILDAPAQYREL
                     LGFLARTVLELRDRLPPDIGAQLPEGAVEIQRAMASYLASKAGTLATTGRAWLSGLLF
                     AYVGLLIGALAAARPSTPGLKPLAAQLHLRVRRFGETFRQIVVAQFWIALFNTCLTAV
                     FLLAILPLTGHRLPYTMALILLTFVAGLIPIVGNLICNGVLTLVGLSVSPGVALACLA
                     FLIAIHKAEYFINAKVIGHRTHMGVWELLSVMFVMEAVFGPAGLVAAPLFYAYGKKEL
                     QASGWV"
     gene            complement(3661..4707)
                     /gene="purM"
                     /locus_tag="PK242_08660"
     CDS             complement(3661..4707)
                     /gene="purM"
                     /locus_tag="PK242_08660"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOZ94130.1"
                     /translation="MSPSSTPSPLSYKDAGVDIDAGDALVERIKPLARRTLREGVLSG
                     LGGFGALFEVPRRYREPVLVSGTDGVGTKLKLAFEWAMHDGVGIDLVAMSVNDVLVQG
                     AEPLFFLDYFACGRLDVDTAATVVGGIARGCELAGCALIGGETAEMPGMYPPGEYDLA
                     GFCVGAVEKSALLTGRDVAAGDVVLGLASSGVHSNGFSLLRKCIERAAGQLPATLDGQ
                     PFRQAVMAPTRIYVKPVLAALAAPGGIKALAHITGGGLLENIPRVLPAGLAAHLRAGS
                     WPRSELFTWLQATAGIDDHEMNRTFNNGIGMVVVVAPAQAAACAQALRAAGEQVFEIG
                     HIAARGDGAAVQVA"
     gene            4873..5559
                     /gene="hda"
                     /locus_tag="PK242_08665"
     CDS             4873..5559
                     /gene="hda"
                     /locus_tag="PK242_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HOZ94131.1"
                     /translation="MRQIALDIGLAPEPTLSGFLAGANAAAVQHLRLWTGGATRSPVP
                     TYLWGPPGSGKTHLLRAARQALAEQGARVGWLDPETPPRLPFDEAWDAVLMDDVQRYG
                     AELQHTAFNWFVNAITPPSGRPRAVLAAGGVPPADLPLREDLRSRLGWGHVFELHPLP
                     EPELRAALRQAADARGVFLSDEVMDFVLRRFARDLGSLMQLLDQLDRYALQAQRAITI
                     PLVKAMLENE"
     gene            5599..6276
                     /locus_tag="PK242_08670"
     CDS             5599..6276
                     /locus_tag="PK242_08670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOZ94132.1"
                     /translation="MLKLALFDLDHTLLPIDSDYAWGEFTTRIGWTDPVDFQRRNDEF
                     YARYQAGTLDVREYVRFATEAVRRQGPEQAARAHARFMREVVGPALRPQARALVEAHR
                     QAGHGLLMVTATNEFVTRPIAQAFGIDELIAIELVRDGAGWITGEIAGTPSFREGKVA
                     RVADWLRARGHDWSDVDITFYSDSRNDLPLLEKAQQPVATNPDDALRAIASERGWRVL
                     ELFKEDT"
     gene            6273..7823
                     /gene="pcnB"
                     /locus_tag="PK242_08675"
     CDS             6273..7823
                     /gene="pcnB"
                     /locus_tag="PK242_08675"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOZ94133.1"
                     /translation="MIKQLIHKLIDSATGGNARKRRGKRVEIGPEGHGINPALVDERA
                     AGVVRTLKEAGYEAYIVGGAVRDLLLGLKPKDFDVATNATPEQVKQLFRRAFIIGRRF
                     RIVHVVFGRGREHEVIEVSTFRAYVDNATAEAIRGNERTAKGQLAGMKHAVDASGRVL
                     RDNVWGSQDEDAARRDFTVNAMYYDPQARVVVDYHGGIADAHKKLLRMIGDPATRYRE
                     DPVRAIRAVRFGAKLAALGFRIEPKTARPLVEVLPLLAEVPQSRLFDEMLKLLQTGHA
                     LASVEQLRKLGLARGIYPLLDVVVERADQPFVKAALQDTDRRVGEGKPVAPSFLLACV
                     LWQDVLAGWNARLARQEHPFPALQDAIDEVFDARVGDVSGRGKLGADMREIWMMQPRF
                     EKRSGQTPFSLVEQPRFRAGFDFMRLRADVGEVSEELADWWQQFSLADDGERESMMTE
                     LRAEQQRSKRQAPRVHRVPAGTLEAAPPAPAPDAAATPAKKRRRRRRKPAGAGGSAPE
                     AGPPPAQP"
     gene            7820..8314
                     /gene="folK"
                     /locus_tag="PK242_08680"
     CDS             7820..8314
                     /gene="folK"
                     /locus_tag="PK242_08680"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOZ94134.1"
                     /translation="MTQPLSHLRAPVLAYVALGANLGDAAASVRAAIEALARLPDTQL
                     QARSSLYRSAPVDAGGPDYVNAVVALRTGLSAPELLAGLQRLEQAAGRQRPYRHAPRT
                     LDLDLLRYGDGRIDSAALCVPHPRMLQRAFVLRPLAEIAPQLVTPAALAAVAGQGIER
                     LEGA"
     gene            8591..>8834
                     /locus_tag="PK242_08685"
     CDS             8591..>8834
                     /locus_tag="PK242_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOZ94135.1"
                     /translation="MLLSKLLPKEGNFFELFNQHADRIVEAANAFSLLVAHYGDLGQR
                     EKYARDVDNAERAADRVTEEVNRLIHTTFITPIDREQ"
BASE COUNT         1284 a         3220 c         3189 g         1141 t
ORIGIN      
        1 atgacgtccg agccgagccc cgcagcgcct gccgcgccgc cccctgtgcc ggcgcccgtg
       61 gcgaccgagg ccggcacggc ggcgccggcc agcccgctgg cgccagcgca tgccgcggca
      121 ccgcgcggcg ccggcgtgtc ccccggcctg tggctggcct ggctgctggc cgcggccggc
      181 ctggtggtcg gcctgctgct gtggcagaag ctgaccggca tgcaggaaca gctggcgcgc
      241 cagagccggg aagccggcac gcagtcggtg gaggcgcgca ccctggcgcg ccaggccgag
      301 gagggcgtgc gcgacatggc cggcaaggtg gcggcgctgg acgcccgcct ggccgaactg
      361 gccgcctacc gggcgcagct cgacgagctg gtgcacagcg tcgcccgcgc gcgcgacgag
      421 aacctggcgg tggatctgga agccgccctg cgcctggcgc aggaccagac ccagctgacc
      481 ggcagcgtgg agccgctgct ggccgcgctg cgcaccgccg agcggcgcct ggcgcgcagc
      541 agcgacccgc gcctggcgcc ggtcggccgc gccgtggcgc gcgacctcga gcgcgtcaag
      601 gcggccagca tccccgacgc cgccggcctg ctggcgcggc tcgatcagct gctgcggcag
      661 gtcgacgatc tgcccgccgc caacgaggtg ccgcacgcgc gcagcgcccc ggccaccacc
      721 gcccccgccg ccgccgccga aagcgcctgg ggccggctgt ggcagagcgt gcgggacgag
      781 gcccagggcc tgctgcgcgt cagccgcatc gaacgccccg aggccagcat gctcagcccc
      841 gagcaggtgt tcttcgtgcg cgaaaacctc aagctgcgcc tgcagggcgc gcgcctggcg
      901 ctgctggcgc gccagtacga agccgcgcgc gccgacctga ccgccgccgc catgtccctg
      961 ggcacctggt tcgacccggc ctcgcgccgc acccaggccg ccgccacctt gctgcagcag
     1021 gtccaggcca gcacccgctc ggccgagctg ccgcgcgtgg acgattccct ggtcgccctg
     1081 gggcaggccg tggccacggc ccacacggtg cccgggacgc gctgaccacc atgcgcgccg
     1141 ccctgtggtt gatgggtctg ttcgccgccg cggtggcgct ggcgctgttt gccggtgaca
     1201 acgaaggcac ggtgacggtg ttctggccgc cgcaccgggt ggacgtgtcg gtcaacctgg
     1261 cgctgctgct gctgctggcg ctgttcgtgc tgctgtacct ggcgctgcgg gcgctgtcgg
     1321 ccatggttca gctgccgcgc caggcgcgcc gctggcgcac ccagcagcgc gagcgcgcca
     1381 tgcacgccac cctgatcgac gctgccgcgc aactggccgc cggacgcttt ctgcgtgcgc
     1441 gcaaggccgc cgaaagcgcg ctgacccggg tgcaggccct gagcgacgcc gccgaagccc
     1501 cgccgcacgc gcccagcctg cgcaccctgg cccacatgac ggtggccgaa agcgcgcacg
     1561 ccttgcgcga cgaggccatg cgcgaccagc atctggaaca cgcgctcgac gtggccggcc
     1621 gtgccggcgc cgcccccgag ctgcgcgaag gcgtgctgct gcgcgccgcg cgctggtcgc
     1681 tcgaccagcg ggacgccggc gcggcgctgg cgcggctcaa cgagctgccc gccggtgcca
     1741 cccggcgcac ggcggcgctg cgcatcaagc tcaaggcggc gcgccagagc gggcaccacg
     1801 ccgaggccct ggaaaccgcg cgcctgctgg ccaagcacgg ggccttttcg ccggccgcgg
     1861 cgcgcagcct gctgcgcggg ctggcggcca gcctgatcga cgaggtgcac gacccggcgc
     1921 agctgcaggc cgtgtggggc tcgctggacg ccggcgagcg cgccatgccc gagctggcgc
     1981 tgcgcgccgc ccagcgcatg ctggcgctgg acggcccgcg cgccctggtg cggcaatggc
     2041 tgcgcccggt gtgggagcag gcgctggccc agccaggcag cttcggcccg gcgctgcgcg
     2101 cccgcctggt gcaggtgctg gagcaggccc tgatcgccac cggcgaggcc tccgaccagc
     2161 aatggctggc gctggtcgag tcggcccagc aggcccaccc gcgcgacccc agcctgcaat
     2221 acctggccgg catggcctgc cgccaccgcg gcctgtgggg ccgggcgcag ctgctgctgg
     2281 gtcaggcggc gcagtcgctg cccgacgccg agctgcgccg ccatgcctgg cgcgcgctgg
     2341 ccgagctggc cgagcagcgc ggcgacgagc ccgccgccgc acaggcctgg aagcaggcgg
     2401 cccggtcctg acggccggcg ccaccggcgg gggggctgta gttccaagga taattggcct
     2461 ccagcgcttg tctggcaagc gccaaaagct attgaataaa tagcaaatcg ggtgccgacg
     2521 gcggtccgaa gccactcccc gggatcctgc cgtcatcgac ccgcagcgag gccatccgtc
     2581 accgatgcat gccggttggc gtcagaccca gcccgaggcc tgcaattcct tcttgccgta
     2641 ggcgtagaac agcggcgccg ccaccagacc ggccggaccg aacacggctt ccatcacgaa
     2701 catcacgctc agcagctccc acacgcccat atgggtgcgg tggccgatga ccttggcgtt
     2761 gatgaagtat tcggccttgt ggatggcgat cagaaaggcc aggcaggcca gcgccacccc
     2821 gggcgacacc gacaggccga ccagggtcag cacgccgttg cagatcaggt tgccgacgat
     2881 cgggatcagg ccggccacga aggtcagcag gatcagcgcc atcgtgtagg gcaggcggtg
     2941 gccggtgagc ggcaggattg ccagcaggaa gacggcggtc aggcaggtgt tgaacagcgc
     3001 gatccagaac tgcgccacca cgatctggcg aaaggtctcg ccgaagcgcc gcacgcgcag
     3061 gtgcaactgg gcggccagcg gcttgaggcc cggcgtgctg gggcgggccg ccgccagcgc
     3121 gccgatcagc agcccgacgt aggcaaacag caggcccgac agccaggcgc gcccggtggt
     3181 ggccagcgtg ccggccttgc tggccaggta gctggccatg gcgcgctgga tctcgaccgc
     3241 gccttcgggc agctgggcgc cgatgtccgg cggcagccgg tcgcgcagct ccagcacggt
     3301 gcgcgccaga aagcccagca gctcgcggta ctgggccggc gcgtccagaa tcagcccgcg
     3361 cgtgcgcggc agcgccagcg ccagcagcag cagcggcagc agcgccacca cggtggcggc
     3421 caggcgcggt gcctggcggt cggcctggcg tgccggcagc gccagccgtg ggctgagcca
     3481 gcgggtgcag aaaaagccca cgcacacgca cagcaggccc ggcagcaggc cctgccacat
     3541 gaccagcagc aggctggcgg cgatcagccc gtggctggcc agggtggcgg tgcgcaggcg
     3601 cgggtcgggc aggggcgcgt gcatcggctg caaggtcgat cgggtagggg gtggccggct
     3661 tcaggccacc tgcacggcgg cgccgtcgcc gcgcgcggcg atgtggccga tctcgaacac
     3721 ctgctcgccg gccgcgcgca gcgcctgggc acaggccgcg gcctgcgccg gcgccaccac
     3781 gaccaccatg ccgatgccgt tgttgaaggt gcggttcatc tcgtggtcgt cgatgccggc
     3841 ggtggcctgc agccaggtga acagctcgct gcgcggccag ctgccggcgc gcaggtgggc
     3901 ggccaggcct gcgggcagca cgcgcgggat gttttcgagc aggccgccgc cggtgatgtg
     3961 cgccagcgcc ttgatgccgc cgggcgccgc cagcgccgcc agcaccggct tgacgtagat
     4021 gcgggtgggc gccatcacgg cctggcgaaa cggctgcccg tccagggtgg ccggcaactg
     4081 gccggcggcg cgctcgatgc acttgcgcaa cagcgagaag ccgttgctgt gcacgccgct
     4141 ggaagccagg cccagcacca cgtcgccggc ggccacgtcg cgcccggtga ggagagcgga
     4201 cttttcgacc gcgccgacgc aaaagcccgc caggtcgtac tcgccgggcg ggtacatgcc
     4261 gggcatttcg gcggtttcgc cgccgatcag ggcgcagccg gccagctcgc agccgcgcgc
     4321 gatgccgccc accaccgtcg ccgccgtgtc cacgtccagt cgcccgcagg cgaagtagtc
     4381 gaggaagaac agcggttcgg cgccctgcac cagcacgtcg ttgacgctca tggccaccag
     4441 atcgatgccg acgccgtcgt gcatggccca ttcgaaagcc agcttcagct tggtgccgac
     4501 gccgtcggtg cccgacacca gcaccggctc gcggtagcgc cgcggcactt cgaacagcgc
     4561 gccgaagccg cccaggccgg acagcacccc ttcgcgcagg gttcgccgcg ccagcggctt
     4621 gatgcgttcg accagcgcat cgccggcgtc gatgtcgaca ccggcatcct tgtagctgag
     4681 gggggagggg gtggaggagg ggctcatcga ggctgggcgg ttgaaaatgg cgggcggtcc
     4741 gcgcgcggga catgcgctgc gtagaatcga ggccagattc taggcgctgc acccccgtgc
     4801 ccccgaccgc gccccgcctg tttccggctt catgcacccc ctgttcatgc cgtttttcgc
     4861 ccctggcgtc cgatgaggca gatcgccctg gacatcgggc tggcgcccga acccaccctg
     4921 agcggctttc tggccggcgc caacgccgcc gccgtgcagc acctgcggct gtggacgggt
     4981 ggcgccacgc gctcgccggt gccgacctac ctgtggggcc cgcccggcag tggcaagacc
     5041 cacttgctgc gcgcggcgcg ccaggcgctg gccgagcagg gcgcgcgggt cggctggctg
     5101 gaccccgaaa ctccgccgcg cctgcccttc gacgaggcct gggatgccgt gctgatggac
     5161 gacgtgcagc ggtacggcgc cgagctgcag cacaccgcct tcaactggtt cgtcaacgcg
     5221 atcacgccgc ccagcggtcg gccgcgcgcc gtgctggcgg ccggcggcgt gccaccggcc
     5281 gacctgccgc tgcgcgagga cctgcgcagc cggctcggct ggggccatgt gttcgagctg
     5341 cacccgctgc ccgagccgga gctgcgcgcc gcgctgcgcc aggccgccga cgcgcgcggc
     5401 gtgttcctgt ccgacgaggt gatggacttc gtgctgcggc gctttgcacg cgatctgggc
     5461 agcctgatgc aactgctcga ccagctcgac cgctacgccc tgcaggccca gcgcgccatc
     5521 accattccgc tggtcaaggc gatgctggaa aatgaatagc aggcggcgcc ctgtccgcct
     5581 gcaccaacca ctgattcgat gctcaaactc gccctgttcg acctcgacca cacgctgctg
     5641 ccgatcgatt cggactacgc ctggggcgag ttcaccaccc gcatcggctg gaccgatccg
     5701 gtcgacttcc agcgccgcaa cgacgagttc tatgcccgct accaggccgg cacgctggac
     5761 gtgcgcgagt acgtgcgctt tgccaccgaa gcggtgcgcc gccagggccc cgagcaggcg
     5821 gcccgggcgc atgcgcgctt catgcgcgag gtggtgggcc cggcgctgcg cccgcaggcc
     5881 cgcgcgctgg tcgaggcgca ccgccaggcc gggcatggcc tgctgatggt gacggcgacc
     5941 aacgaattcg tcacacgccc gatcgcccag gcgttcggca tcgacgagct gatcgccatc
     6001 gagctggtgc gcgatggcgc cggctggatc accggcgaaa tcgccggcac cccctcgttt
     6061 cgcgaaggca aggtggcgcg cgtggccgac tggctgcgcg cccgcgggca cgattggagc
     6121 gacgtcgaca tcaccttcta ttccgactcg cgcaacgacc tgccgctgct ggaaaaggcg
     6181 cagcagccgg tggccaccaa cccggacgac gcgctgcgcg ccatcgccag cgagcgcggc
     6241 tggcgcgtgc tggagctgtt caaggaagac acatgatcaa gcaactgatc cacaagctga
     6301 tcgacagcgc caccggcggc aacgcgcgca agcggcgcgg caagcgggtc gaaatcggcc
     6361 ccgagggcca cggcatcaac ccggcgctgg tggacgagcg tgccgccggc gtggtgcgca
     6421 cgctcaagga agccggctac gaggcctaca tcgtcggcgg cgccgtgcgc gatttgctgc
     6481 tggggctcaa gcccaaggac ttcgacgtcg ccaccaacgc cacgccggag caggtcaagc
     6541 agctgttccg gcgcgccttc atcatcgggc gccgctttcg catcgtgcac gtggtgttcg
     6601 gccgcgggcg cgagcacgag gtgatcgagg tgtccacctt ccgcgcctac gtggacaacg
     6661 ccaccgccga ggccatccgc ggcaacgaac gcaccgccaa gggccagctg gccggcatga
     6721 agcacgcggt agacgccagc gggcgggtgc tgcgcgacaa cgtctggggc agccaggacg
     6781 aagacgcggc gcggcgcgac ttcaccgtca acgccatgta ctacgacccg caggcgcgcg
     6841 tggtggtgga ctaccacggc ggcatcgccg acgcgcacaa gaagctgctg cgcatgatcg
     6901 gcgatcccgc cacgcgctac cgcgaagacc cggtgcgcgc catccgcgcc gtgcgctttg
     6961 gcgccaagct ggcggcgctg ggctttcgta tcgaacccaa gaccgcccgc ccgctggtcg
     7021 aagtcctgcc gctgctggcc gaggtgccgc aaagccgcct gttcgacgaa atgctcaagc
     7081 tcctgcagac cggccacgcg ctggccagcg tcgagcagct gcgcaagctg gggctggcgc
     7141 gcggcatcta cccgctgctg gacgtggtgg tcgagcgcgc cgaccagccc ttcgtgaagg
     7201 cggcgctgca agacaccgac cggcgcgtgg gcgaaggcaa accggtggcg ccctcgttcc
     7261 tgctggcctg cgtgctgtgg caggacgtgc tggcgggctg gaatgcgcgg ctggcgcgcc
     7321 aggaacaccc gttcccggcg ctgcaggacg ccatcgacga ggtgttcgac gcccgcgtcg
     7381 gtgacgtgtc cgggcgcggc aagctgggcg ccgacatgcg tgaaatctgg atgatgcagc
     7441 cgcgctttga aaagcgcagc ggccagacgc cgttcagtct ggtcgagcag ccgcgctttc
     7501 gcgccggctt cgacttcatg cgcctgcgcg ccgacgtggg cgaggtgagc gaggagctgg
     7561 ccgactggtg gcagcagttc agcctggccg acgacggcga gcgcgaaagc atgatgaccg
     7621 agctgcgtgc cgagcagcag cgcagcaaac gccaggcgcc gcgcgtgcac cgggtgccgg
     7681 cgggcacgct ggaggcggcg ccacccgcgc ccgcccccga cgccgccgcc accccggcca
     7741 agaagcgccg gcggcgccgt cgcaagcctg ccggcgcggg cggatcggcg cccgaggccg
     7801 gcccgccgcc ggcccagccg tgacgcagcc gctgtcccat ctgcgcgcgc cggtgctggc
     7861 ctatgtggcg ctgggcgcca acctgggcga cgcggccgcc agcgtgcgcg ccgccatcga
     7921 ggcgctggcg cggctgccgg acacgcagct gcaggcgcgc tccagcctgt accgcagcgc
     7981 gccggtggac gccggcgggc cggactacgt caacgccgtg gtggcactgc gcaccggcct
     8041 gtcggcgccc gagctgctgg ccgggttgca gcggctggag caggcggccg gccgccagcg
     8101 gccgtatcgc cacgcgccgc gcacgctgga cctggacctg ctgcgctatg gcgacggccg
     8161 catcgacagc gctgcgctgt gcgtgccgca cccgcgcatg ctgcagcgcg ccttcgtgct
     8221 gcgtccgctg gccgagattg cgccgcagct ggtcacgcca gcggcgctgg cggccgtggc
     8281 cgggcagggc atcgaacgtc tggagggcgc atgagccctg ggcggtccat gccggattcg
     8341 gagccaaacc gggctccagc gcatatcaat actgcgccag cagctatgat tattgtagct
     8401 tttcgaacgc gacaccgggt gcggccgacc gcccggcggt gccaaggcgt ctcagcggtg
     8461 gggcagtggt tgatttgcca cgtgtgtcag ccagcgccgc agtcggggtg gttaaactca
     8521 ccggatcgtc gtgggcgctg cgatccagtg gtccgcagac tttcgattta ctgaagcaag
     8581 gttatccctc atgctgctca gcaagctgtt gcccaaggag ggcaattttt ttgagctgtt
     8641 caaccagcac gccgaccgca tcgtcgaagc cgccaatgcc ttttcgctgc tggtggcgca
     8701 ctacggcgac ctgggacagc gcgaaaaata cgccagggac gtggacaacg ccgagcgcgc
     8761 cgccgaccgt gtgaccgaag aggtcaaccg cctcatccac accaccttca tcaccccgat
     8821 cgaccgcgag caga
//
LOCUS       DAOOOG010000105         8791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_9342_length_8791_cov_31.142514, whole genome shotgun sequence.
ACCESSION   DAOOOG010000105 DAOOOG010000000
VERSION     DAOOOG010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9342_length_8791_cov_31.142514"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="PK242_08690"
     CDS             complement(<1..1330)
                     /locus_tag="PK242_08690"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOZ94136.1"
                     /translation="MNAPLTPGMIQDLHVPPYVQHARLIAWVGDMVALCKPDRVHWCD
                     GSQAEYERLCQQLVEAGTLKKLNPAKRPGSFLACSDPSDVARVEDRTFICSERQQDAG
                     PTNNWEPPAEMRAKLEPLFDGCMRGRTMYVIPFSMGPLGSPIAHIGVELSDSAYVAVN
                     MKLMTRMGRGAIEVLGDNGDFVPCVHTVGAPLAPGQQDQTAWPCNPEVKYIVHYPETR
                     EIWSYGSGYGGNALLGKKCFALRIASKMGQEQGWLAEHMLILGVTSPEGKKYHVAAAF
                     PSACGKTNFSMLVPPAGFEGWKVTTIGDDIAWIKPGPDGRLYAINPEAGYFGVAPGTN
                     LHTNPNCMHSLDRDVIFTNVALTDDGDVWWEGMEKDTGVLPEHLIDWQGKDWTPADGQ
                     AGRKAAHPNARFTVAATNNPVLDAAWDDPAGVPIDAFIFGGRRSTTVPLVT"
     gene            complement(1596..1730)
                     /locus_tag="PK242_08695"
     CDS             complement(1596..1730)
                     /locus_tag="PK242_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94137.1"
                     /translation="MSASTEHLTSEDPVESVTRSIPVVIPLAGAVLIFLLAFIAVTVA
                     "
     gene            complement(1827..3377)
                     /locus_tag="PK242_08700"
     CDS             complement(1827..3377)
                     /locus_tag="PK242_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOZ94138.1"
                     /translation="MADWNTMWAGLRGTLGIHVLQLLGALLIVIVGWFLAALVKAGAR
                     KGLRLLRLNERFAGTSGQRADLEGAIALALFWLVMLLTLGAMFGALELSQVSGSFAAL
                     NLQLFEYAPRVLGAALLALLAWLVAALVRGLARKLLDRTTLDDKLSEHAHIAPISENL
                     SNALFWLVVLLFVPAILGTLQMDGLLLPLRDMTARAVDILPDIVAAVVIGGVGWVVSM
                     VLRNLTSNLLRSAGFDQLGSKLGLAAGVQLSRLAGLLVFVLVFVPALIAALDALRIEA
                     ISRPATDMLGVLLDAVPRIMAAGLILLLTWLVATLATRLLSSLLASLGLDTLPARLGL
                     QQAFARTPPSQLIGRVGLLFAMLFAVVEASNQLGFQRLADLVTTFIEFAGDVLLGSLI
                     LIVGFMLANAAYEAISRASGRHTPLARLARLAILGIVLAMGLRAMGIADDIVNLAFGL
                     TLGAIAVAVALAFGLGGREAAARLAAHWVDRWTREQAADEAAATPPPSPPPPPEPEPE
                     PAPPGDRP"
     gene            3565..5904
                     /locus_tag="PK242_08705"
     CDS             3565..5904
                     /locus_tag="PK242_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ94139.1"
                     /translation="MTKRTTTTVREPAGMLATPGLAAAPVMDLMCSHFVLTLAAQQGS
                     KFNVRRDINGLMALAGRHLMWPQAVLARLREFLARRCKDNEFWRGHERLSDAEFLERH
                     GAWRGPYEEGTLFFYLDEYAKDAPKDLLALLAATGQWLRHALKKQSTLVEKNIDALAN
                     LLQLNKAERALLLYGTLARYQRDLRSLLVEFKVNNAPEAYAAIGDLAGVSAAEVGEAL
                     RAGGRLERIGLVENLISEHSITDLADLMKVSEKLPPVLMREYRDRAELMAVFTRPASK
                     SSLKASDFSFVGDDVQVLIQLLRKAQQTQAAGVNVLLYGPPGTGKTELAKVVAREAGL
                     DLFEVEYADRDGNSLSGRDRYRSMQIAQVFLKGAHEAALLFDEVEDVFPPISVDAAGL
                     MARAEQQALSAAVSHSVNGKAWVNQILESNPVPTLWVTNRIEQIDPAFRRRFAYHLEL
                     KSPPPGAREQLVRRALDGVEVSEALVVRLTERKGLTPAQIRTAVRFADLAHADDARGG
                     AAFEALIERQLKNADAALGRSGESAPAKGRRSVTTYDLGMLNVESRFEVPRIVEALKA
                     RGHGALCFYGAPGTGKTALGEYIAEQMGKPLIVRQASDLVSKFVGETEQNMAAMFKEA
                     SEEKAVLLLDEADSFLLDRRGAQRSYEVTEVNEMLQQMERHDGVFICTTNLLDRIDQA
                     ALRRFTFKIRFKPLTAEQREHMFVTEALDGQADALTDDARRRLARLEQLCPGDFAAVK
                     RQVDILASRLTPEEFLEQLESEHRIKPEVREQRSMGFLH"
     gene            complement(5996..6886)
                     /locus_tag="PK242_08710"
     CDS             complement(5996..6886)
                     /locus_tag="PK242_08710"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOZ94140.1"
                     /translation="MTSTTRSKHALPSRASTRMRTPWLLASAGAALLALTLTLRQEAV
                     ARQTVDLGLPPPLMLAALLLLLALGFGILAWPARRRAGVGVRRPLASPDWSRLTQMAN
                     WFFDAHGLVREGATRPWAAGQTLLLHNESHRFLVQTKLWRARRVDAAAVQRLSREMAR
                     RGADRGMLLCAGDVFTAAARQLARRSGILLLEPQQLSSRPSAAPRPDARSQAATLSTL
                     PPPAEPAPVRAEAPTVLLSLDDAAPPRFEFMPTVPMSKAERLAAVAPVLRPDHEVARG
                     LCEFMPTVPSALSPSAATQA"
     gene            complement(7073..>8791)
                     /locus_tag="PK242_08715"
     CDS             complement(7073..>8791)
                     /locus_tag="PK242_08715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOZ94141.1"
                     /translation="ALHEAGVAAAFLNSTLDWAQAEDIERRLLAGQLTLLYAAPERVA
                     TPRFLGLLDTLAAHGQLSLFAIDEAHCVSQWGHDFRPEYRQLTVLHERWPQVPRIALT
                     ATADALTRADIVERLQLQDARPFVSSFDRPNIFYRIVEKKDATRQLLQFIEQQHEGEA
                     GIVYCQSRKRVEELAATLCQHGIAALPYHAGLPADERKQNQERFQREDGVVMCATIAF
                     GMGIDKPDVRFVAHVDLPKNIEGYYQETGRAGRDGAPADAWMAYGLQDVVNQRRMIDE
                     SPAEEDFKQVMRGKLDALLALAEATDCRRARLLAYFGEQMVSRPREDTIDSIAIRATP
                     ASASGQNGSYPQPLAYCGGCDNCLQPPQVWDATDCARKLLSTVYRVQQMSGIGFGAGH
                     LMDILRGKATDKVRQFGHEQLSTFGLGAEFSEAQLRAVLRQLIASGALHVDAERYHTL
                     QLQDGARPILRGEQAVRLRADVAAPAARQRPRKAARGATPAPAAASLDAAAQQRFADL
                     KAWRGEVAREHNLPAYVIFHDATLAAIAERAPQSLAELQGISGIGASKLERYGDEVLR
                     VARGAG"
BASE COUNT         1364 a         3021 c         3148 g         1258 t
ORIGIN      
        1 cggtcaccag cggcacggtg gtggagcggc ggccgccgaa gatgaaggcg tcgatcggca
       61 cgccggccgg gtcgtcccag gcggcgtcga gcaccgggtt gttggtggcg gccacggtga
      121 agcgcgcgtt cgggtgcgcc gccttgcggc cggcctggcc gtcggccggg gtccagtcct
      181 tgccctgcca gtcgatcaag tgctcgggca gcacgccggt gtccttttcc atgccttccc
      241 accagacgtc gccgtcgtcg gtgagcgcga cgttggtgaa gatcacgtcg cggtccagac
      301 tgtgcatgca gttggggttg gtgtgcaggt tggtgccggg cgccacgccg aagtagccgg
      361 cctcggggtt gatggcgtac agccggccgt ccgggccggg cttgatccag gcgatgtcgt
      421 cgccgatggt ggtgaccttc cagccttcaa agccggccgg cggcaccagc atcgagaagt
      481 tggtcttgcc gcaggcgctg ggaaaggcgg cggccacgtg gtacttcttg ccttccgggc
      541 tggtcacgcc caggatcagc atgtgctcgg ccagccagcc ctgctcctgg cccatcttgg
      601 aggcgatgcg cagggcaaag cacttcttgc ccagcagcgc gttgccgccg tagcccgagc
      661 cgtaggacca gatttcgcgc gtttcggggt agtggacgat gtacttgacc tcggggttgc
      721 agggccaggc ggtctggtcc tgctggcccg gcgccagcgg cgcgcccacc gtgtgcacgc
      781 agggcacgaa gtcgccgttg tcgcccagca cctcgatggc gccgcgaccc atgcgggtca
      841 tcagcttcat gttgacggcc acgtaggcgc tgtcggacag ctcgaccccg atgtgggcga
      901 tcgggctgcc cagcgggccc atgctgaagg ggatgacgta catggtgcgc ccacgcatgc
      961 agccgtcgaa cagcggctcc agcttggcgc gcatttcggc cggcggctcc cagttgttgg
     1021 tggggccggc gtcctgctgc ctttcggagc agatgaaggt gcggtcttcc acccgcgcca
     1081 cgtcggacgg gtcggagcag gccagaaacg accccgggcg cttggcgggg ttgagcttct
     1141 tgagggtgcc ggcctcgacc agctgctggc acagccgctc gtactcggcc tggctgccgt
     1201 cgcaccagtg cacgcggtcg ggcttgcaca gggccaccat gtcgcccacc caggcgatca
     1261 ggcgggcatg ctggacgtac ggcgggacgt ggagatcctg gatcatcccg ggggtcaggg
     1321 gtgcgttcat cgacaaagct cctgaaatga aaatcgctct ttccgaaggc gccaggcctt
     1381 gggaaagccg actcgaaccc tggtcgaccc gcacggacgc tgcggcgacg gcacgcccct
     1441 ttgcagccac gggcaaaggg ggatgatttt atgaaatccg ccccacgcga tcctgacggc
     1501 cctgccgtgg gccgggaggg cgcgccgcaa gctatgattt caatagctgc tggcgctttc
     1561 ctggtgggac ttgaaggccg atttggcgct gaaactcagg cgacggtgac ggcgatgaag
     1621 gccagcagga agatcagcac cgcgccggcc agcgggatga ccacggggat gctccgggtc
     1681 acggattcca ccgggtcttc ggaggtcaga tgttcggtgc tggcagacat gggaatggcc
     1741 tttgggttcg gaatcgggga tagcggggca ttctatgcgc gccacctgcg gcttgcatgg
     1801 ggtcaaaccc ggcgccgggg gcgccctcag ggccgatcgc cgggcggcgc gggctcgggc
     1861 tcgggctcgg gtggcggtgg tggcgacggc ggcggggtgg ccgccgcttc gtcggcggcc
     1921 tgctctcggg tccagcgatc gacccagtgc gccgccagac gggcggcggc ttcgcgcccg
     1981 cccagcccga aggccagcgc cacggccacc gcgatggcgc ccagcgtcag gccgaaagcc
     2041 aggttgacga tgtcgtcggc aatgcccatg gcgcgcaggc ccatggccag cacgatgccc
     2101 aggatggcca gacgcgccag ccgggccagc ggcgtgtggc ggccgctggc gcgcgaaatc
     2161 gcttcgtagg cggcgttggc cagcatgaag ccgacgatca ggatcagcga gcccagcagc
     2221 acgtcgccgg cgaattcgat gaaggtcgtc accaggtcgg ccaggcgctg aaagcccagc
     2281 tggttgctcg cctcgaccac cgcgaacagc atggcaaaca gcaggccgac gcgaccgatc
     2341 agctgcgacg gcggcgtgcg cgcaaaggcc tgctgcagac cgagccgcgc cggcagggtg
     2401 tccagaccga ggctggccag caggctggac agcaggcgcg tggccagcgt ggccaccagc
     2461 caagtcagca gcaggatcag cccggcggcc atgatgcgcg gcacggcgtc cagcagcacc
     2521 cccagcatgt cggtggcggg gcgcgagatg gcctcgatgc gcagcgcgtc cagcgcggcg
     2581 atcagcgccg gcacgaacac cagcacgaac accagcagcc cggccaggcg cgacagctgc
     2641 acgccggcgg ccagccccag cttgctgccc agctggtcga aaccggccga gcgcagcagg
     2701 ttgctggtca ggttgcgcag caccatcgac accacccagc cgacgccacc gatcaccacc
     2761 gccgccacga tgtcgggcag gatgtcgacc gcccgcgccg tcatgtcgcg cagcggcaac
     2821 agcaggccgt ccatctgcag cgtgcccagg atggccggca cgaacagcag caccaccagc
     2881 cagaacaggg cgttggacag gttttcgctg atcggcgcga tgtgggcgtg ctcggacagc
     2941 ttgtcgtcca gcgtggtgcg gtccagcagc ttgcgcgcca gaccgcgcac cagcgccgcc
     3001 accagccagg ccagcaaggc cagcagcgcc gcccccagca cgcgcggcgc atattcgaac
     3061 agttgcaggt tgagcgccgc gaacgagccc gacacctgcg acagctcgag cgcaccgaac
     3121 atcgccccca gcgtcagcag catcaccagc cagaacagcg ccagcgcgat cgcgccctcc
     3181 aggtcggcgc gctggcccga ggtgccggca aagcgctcgt tcaggcgcag caggcgcagg
     3241 cccttgcgcg cccccgcctt caccagcgcc gccaggaacc agccgacgat gacgatcagc
     3301 aaggccccca gcagttgcag cacgtggatc cccagcgtgc cgcgcaggcc cgcccacatc
     3361 gtgttccagt ccgccatgtc gatgcttctc ctgtgttgcc gcctattgtg accccctgcc
     3421 gcgccgccag cgccggccgg ccgccgagcg tgacgaaatg ccgccaccgg cgccgcggac
     3481 gtgaccggcc ggcgtcttcc gcgcctgcgt ggattgcgtt atggtggcgc ttgatctggc
     3541 gctcaacctt cagggaggtc gctgatgacg aaacggacga cgacgacggt gcgcgaaccc
     3601 gcgggcatgc tggccacgcc cggtctggcg gctgcgccgg tgatggatct gatgtgttcg
     3661 cacttcgtgc tgacgctggc ggcccagcag ggcagcaagt tcaacgtgcg gcgcgacatc
     3721 aacggcctga tggcgctggc cggccgccac ctgatgtggc cccaggcggt gctggcgcgg
     3781 ctgcgcgagt tcctggcccg gcgctgcaag gacaacgaat tctggcgcgg ccacgagcgg
     3841 ctgtcggacg ccgaattcct ggagcgccac ggcgcctggc gtggccccta cgaggaaggc
     3901 acgctgttct tctacctgga cgaatacgcc aaggacgccc ccaaggacct gctggcgctg
     3961 ctggccgcca ccggccagtg gctgcgccat gcgctcaaga agcagtccac cctggtcgag
     4021 aagaacatcg acgcgctggc caatctgctg cagctgaaca aggccgagcg cgcgctgctg
     4081 ctgtacggca cgctggcgcg ctaccagcgc gatctgcgca gcctgctggt ggagttcaag
     4141 gtcaacaacg cgcccgaggc ctatgccgcc atcggcgatc tggccggcgt cagcgccgcc
     4201 gaggtgggcg aggcgctgcg cgccggcggg cggctggagc gcatcggcct ggtcgagaac
     4261 ctgatttccg agcacagcat caccgacctg gccgacctga tgaaggtcag cgaaaagctg
     4321 ccgccggtgc tgatgcgcga gtaccgcgac cgcgccgagc tgatggcggt gttcacccgc
     4381 ccggccagca agagcagcct gaaggcgagc gatttcagct tcgtcggcga cgacgtgcag
     4441 gtgctgatcc agctgctgcg caaggcccag cagacccagg ccgccggcgt caacgtgctg
     4501 ctgtacggcc cgccgggcac cggcaagacg gagctcgcca aggtggtggc gcgcgaggcc
     4561 gggctggacc tgttcgaggt cgaatacgcc gaccgcgacg gcaacagcct gtccgggcgc
     4621 gaccgctacc gctcgatgca gatcgcccag gttttcctca agggcgcgca cgaagcggcg
     4681 ctgctgttcg acgaggtgga ggacgtgttt ccgcccatca gcgtcgacgc cgccggcctg
     4741 atggcgcgcg ccgagcagca ggcgctgtcg gcggcggtga gccacagtgt caacggcaag
     4801 gcctgggtca atcagatcct cgaatccaac ccggtgccca cgctgtgggt gaccaaccgc
     4861 atcgagcaga tcgacccggc gttccggcgc cgctttgcct accacctgga gctgaagtca
     4921 ccgccgcccg gcgcgcgtga gcagctggtg cgccgggcgc tcgacggggt cgaggtcagc
     4981 gaggccctgg tggtgcggct gaccgagcgc aagggcctga cgccggcgca gatccgcacc
     5041 gcggtgcgct ttgccgattt ggcgcatgcc gacgacgccc gcggcggcgc cgccttcgag
     5101 gcgctgatcg agcgccagct gaagaacgcc gacgccgcgc tggggcgcag cggcgagagc
     5161 gcgccggcca aggggcggcg cagcgtcacc acctacgacc tgggcatgct gaacgtcgaa
     5221 agccgcttcg aggtgccgcg catcgtcgag gcgctgaagg cgcgcggcca tggcgcgctg
     5281 tgcttctacg gcgcaccggg caccggcaag accgcgctgg gtgaatacat tgccgagcag
     5341 atgggcaagc cgctgatcgt caggcaggcc agcgacctgg tgagcaagtt cgtcggcgag
     5401 accgagcaga acatggccgc catgttcaag gaagccagcg aggaaaaggc ggtgctgctg
     5461 ctggacgaag ccgacagctt tctgctggac cgacgcggcg cccagcgcag ctacgaagtc
     5521 accgaggtca acgagatgct gcagcagatg gagcgccacg acggcgtgtt catctgcacc
     5581 accaacctgc tcgatcgcat cgaccaggcg gcgctgcggc gcttcacctt caagatccgc
     5641 ttcaagccgc tcaccgccga gcagcgcgag cacatgttcg tcaccgaggc gctggacggc
     5701 caggccgacg cgctgaccga cgacgcgcgc cgccgcctgg cccggctgga gcagctgtgc
     5761 ccgggcgact tcgccgccgt caagcgtcag gtggacatcc tggccagccg cctgacgccg
     5821 gaggaatttc tcgagcagct cgaatcggag caccgcatca agcccgaggt gcgcgagcag
     5881 cgctcgatgg gctttctgca ctgagcctgt tgcttcggaa acttgatgtt tgaacctaga
     5941 aacggcttcg cgcccaagaa cgcgaccgat gacaccggct gcgccggttc gtgaatcaag
     6001 cctgcgtcgc ggcggacggc gacagcgcgc tcggcacggt cggcatgaat tcgcacagcc
     6061 cgcgcgccac ctcgtggtcg gggcgcagaa ccggagccac ggcggccagg cgctcggcct
     6121 tgctcatcgg cacggtgggc atgaattcga accggggcgg cgccgcgtca tcgagcgaca
     6181 gcaggacggt gggtgcttcg gcgcgcacgg gcgcgggctc ggcgggcggg ggcagggtgc
     6241 tgagtgtcgc ggcctgcgac ctggcgtcgg gccgcggtgc ggcactcgga cggctggaca
     6301 gctgctgcgg ctcgagcagc aggatgccgc tgcggcgggc aagctgccgc gccgccgccg
     6361 tgaacacgtc gccggcgcac agcagcatgc cccgatcggc cccgcggcgc gccatttcgc
     6421 gcgacagccg ctgcaccgca gcggcgtcga cccggcgcgc ccgccacagc ttggtctgca
     6481 ccagaaagcg gtgggattcg ttgtgcagca gcagggtttg gccagccgcc cagggcctgg
     6541 tggcgccctc gcgcaccagg ccatgggcat cgaagaacca gttggccatc tgggtcagcc
     6601 gcgaccagtc cggtgacgcc agcggtcgcc gcacgcccac gccggcgcgc cggcgcgccg
     6661 gccaggccag aatgccgaac ccgagcgcca gcagcagcag cagcgccgcc agcatcaagg
     6721 gcggcggcag gcccagatcg acggtctggc gcgccaccgc ctcctgccgc agcgtcagcg
     6781 tcagcgccag cagcgccgcg ccggcgctgg ccagcaacca gggcgtgcgc atgcgggtcg
     6841 aggcccgcga gggcagagcg tgcttgctcc gcgtggtgga tgtcatgtga ttctccctgc
     6901 gggcagcggg tgcccagcgg cattgtcggc gcgccggggc gctgatgtct gtgtcacagt
     6961 tcacgcgccg cgcgtcggcg ccggcgccgc accccgccac ggggcgacgg cgtgcaatag
     7021 tgcgcagtcg cggcgcggcg ggcgccgccg ggtcgtggcc ggcaggcgac gctcaaccgg
     7081 ccccgcgcgc cacccgcagc acctcgtcgc cgtagcgctc cagcttgctg gcgccgatgc
     7141 cgctgatgcc ctgcagctcg gccagcgact gcggtgcgcg ctcggcgatg gcggccaggg
     7201 tggcgtcgtg aaagatcacg taggcgggca ggttgtgctc gcgcgcgact tcgccgcgcc
     7261 aggccttgag gtcggcaaag cgctgctggg cggcggcgtc caggctggcc gcggcgggcg
     7321 cgggcgtggc gccgcgcgcc gccttgcgtg ggcgctggcg cgccgccggc gcggccacgt
     7381 cggcgcgcag ccgcaccgcc tgctcgccgc gcaggatggg gcgcgcgccg tcttgcagct
     7441 gcagcgtgtg gtagcgctcg gcatcgacgt gcagcgcgcc gctggcgatc agctggcgca
     7501 gcacggcgcg cagctgcgcc tcgctgaact cggcgcccaa tccgaaggtg ctgagctgct
     7561 cgtgaccgaa ctggcgcacc ttgtcggtgg ccttgccgcg caggatgtcc atcaggtggc
     7621 cggcgccaaa accgatgccg ctcatctgct gcacgcgata gacggtggac agcagcttgc
     7681 gcgcgcagtc ggtggcgtcc cagacctggg gcggctgcag gcagttgtcg cagcccccgc
     7741 agtaggcgag gggctgcgga taggagccat tttggccgct ggcgcttgct ggtgttgcgc
     7801 gaatagctat cgaatcaata gtatcctcac gcggccggga caccatctgc tcgccaaagt
     7861 aggccagcag ccgcgcacgc cggcagtcgg tggcctcggc cagcgccagc agcgcgtcga
     7921 gcttgccgcg catcacctgc ttgaagtctt cctcggccgg gctttcgtcg atcatgcgcc
     7981 gctggttgac cacgtcctgc aggccatagg ccatccaggc gtcggccggc gcgccgtcgc
     8041 gcccggcgcg gccggtctcc tggtaatagc cctcgatgtt cttgggcaga tcgacgtggg
     8101 cgacaaagcg cacgtcgggc ttgtcgatgc ccatgccgaa ggcgatggtg gcgcacatca
     8161 ccaccccgtc ctcgcgctga aagcgctcct ggttctgctt ccgctcatcg gccggcaggc
     8221 cggcgtggta gggcagcgcg gcgatgccgt gctggcacag cgtggccgcg agttcttcca
     8281 cccgcttgcg cgactggcag tagacgatgc cggcctcgcc ctcgtgctgc tgttcgatga
     8341 actgcagcaa ctggcgcgtg gcgtccttct tctcgacgat gcgatagaag atgttgggcc
     8401 ggtcgaagct gctgacgaag gggcgcgcat cctgcagttg caggcgctcc acgatgtcgg
     8461 cgcgcgtcag cgcatcggcg gtggccgtca gcgcgatgcg cggcacctgc ggccagcgct
     8521 cgtgcagcac ggtgagctgc cggtactcgg gccggaagtc gtggccccac tggctgacgc
     8581 agtgcgcctc gtcgatggca aacaaactga gctgcccgtg cgccgccagc gtgtccagca
     8641 ggcccagaaa gcgcggcgtg gccacccgct cgggcgcggc gtacagcagg gtcagctggc
     8701 cggccagcag gcggcgctcg atgtcctcgg cctgcgccca gtccagcgtc gaattgagaa
     8761 acgccgccgc cacgcccgcc tcgtgcagcg c
//
LOCUS       DAOOOG010000106         8790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_9344_length_8790_cov_20.626102, whole genome shotgun sequence.
ACCESSION   DAOOOG010000106 DAOOOG010000000
VERSION     DAOOOG010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9344_length_8790_cov_20.626102"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PK242_08720"
     CDS             <1..231
                     /locus_tag="PK242_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOZ94142.1"
                     /translation="GIGRWTAEMFLMFHLLRPDVLPLDDVGLINGISLNYFSGEPVSR
                     SEAREVAAAWAPWRTVATWYMWRSLEPLPVAY"
     gene            284..1255
                     /locus_tag="PK242_08725"
     CDS             284..1255
                     /locus_tag="PK242_08725"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOZ94143.1"
                     /translation="MAKRAFLDFEQPIAELENKIEELRYVQSESAVDISEEIDQLDKK
                     SRQLTKDIYADLTPWQITKIARHPERPYTLDYVRECFTDFVELHGDRHYADDQSIVGG
                     LARFGGNACLVIGHQKGRDTKERAARNFGMVRPEGYRKALRLMKTAEKFKLPVFTFVD
                     TPGAFPGIDAEERGQSEAIGRNIYEMAQLEVPIISTIIGEGGSGGALAIAVADQVLML
                     QYSIYSVISPEGCASILWKTSDKAQEAAEALGITAHRLKALGMVDKIVSEPVGGAHRD
                     PRHMGTLLKRALGDAWRQVSDLKVKELLDRRYERLMSYGRFNDTKER"
     gene            complement(1366..1659)
                     /locus_tag="PK242_08730"
     CDS             complement(1366..1659)
                     /locus_tag="PK242_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOZ94144.1"
                     /translation="MWKLGRLIALLRKELLLAWGLLRDPRAPLASKLATVLALLYVLS
                     PVDLVSDFIPILGWLDDGLIAWLLLRLAFRFLPPELMTALRAKVQSRVTPARG"
     gene            1823..2392
                     /gene="phaR"
                     /locus_tag="PK242_08735"
     CDS             1823..2392
                     /gene="phaR"
                     /locus_tag="PK242_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HOZ94145.1"
                     /translation="MSRAPAPAPAKPARKPSTSAPSQRVIKKYPNRRLYDTDTSTYIT
                     LAQVRELVMARQPFVVRDAKTGDELTRSILLQIILEEEAGGAPMFTQDMLANIIRFYG
                     HAMQGFMGSYLEKNIQAFMEIQAKLAEQSSGLNPELWAQFMHMQSPMMQGMMGNYLEQ
                     SKNMFLQAQEQMSKQTEQMLGAFGIKRGA"
     gene            2430..3848
                     /gene="rimO"
                     /locus_tag="PK242_08740"
     CDS             2430..3848
                     /gene="rimO"
                     /locus_tag="PK242_08740"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOZ94146.1"
                     /translation="MSETTTPPRIGFVSLGCPKALTDSELILTQLSAEGYQTSKTFEG
                     ADLVIVNTCGFIDEAVKESLDTIGEALTVNGKVIVTGCLGAKTIAAGADGTGAKGQKD
                     TENLVKHLHPSVLAVTGPHATQEVMDAVHRHLPKPHDPFLDLVPGAFGEAGLKLTPRH
                     YAYLKISEGCNHRCTFCIIPSMRGDLVSRPIGEVLKEARALFEGGVKELLIISQDTSA
                     YGVDVKYRTGFWDGRPLKTRLLELVQALGELAAPHGAWVRLHYVYPYPHVDDILPLMA
                     EGKVLPYLDVPLQHSHPDVLRRMKRPASGEKNLERIARWRDICPELVIRSTFIAGFPG
                     ETEAEFEHLLDFMREARIDRAGCFAYSPVEGAAANALPGMLPPELREERRARFMAVAE
                     AVSTAKLRERVGDTLQVLIDSAPALGRRGGVGRSYADAPEIDGVVHLLPPQKISKQLR
                     VGEFTRARVVDTQGHDLIAQPL"
     gene            complement(3969..4053)
                     /locus_tag="PK242_08745"
     tRNA            complement(3969..4053)
                     /locus_tag="PK242_08745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4017..4019),aa:Leu,seq:cag)
     gene            4105..6372
                     /gene="rnr"
                     /locus_tag="PK242_08750"
     CDS             4105..6372
                     /gene="rnr"
                     /locus_tag="PK242_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOZ94147.1"
                     /translation="MLEEIEGTVQGHRDGHGFVLRDDGDADIYLPPNEMRAVLHRDRV
                     RVRIVRQDRRGRPEGRVLEIVERPPQPIIGRLLQEGGIWLVAPEDRRYGQDVLIPKTA
                     IGLARPGQVVVVELTEPPALYGQPVGRVKEVLGDIDDPGMEIEIAVRKYGVPHEFSDA
                     AIGEARALPDSVQPRDRAGRVDLTDVPLVTIDGEDARDFDDAVYCQPAVVTGRGKKPN
                     GWRLLVAIADVSHYVRNGAPLDVDAYDRATSVYFPRRVIPMLPEKLSNGLCSLNPEVE
                     RLSMVCDMLVTAQGDVHAYQFYPAVIRSHARLTYTEVAAILANTRGPEAARRQARVQN
                     LLDLHDAFRALLKARQRRGAVDFDTVETQIVCDEAGRIEKIVPRQRNDAHRLIEECML
                     AANVCSADFIGQGKHAGLYRVHEGPTPEKLELLRAYLKALGLGLSVSDEPRPDEFQRI
                     AAATAERPDAEQIHMMLLRAMSQAIYSPVNSGHFGLAYEAYTHFTSPIRRYPDLLVHR
                     VIKAILAERKYQLPSLPTPGEAHEKLSKRLKKDSLSRKREQRERPDRLSPEVQAWEAA
                     GLHCSANERRADEASRDVEAWLKCKYMKEHLGEEYAGTVSAATSFGLFVTLSELHVEG
                     LVHITELGGEYFRFDEVRQELRGERTGIRYAIGTRVRVQVSRVDLDARKIDFRLVRED
                     EDLLARAPRDKGAAPAAERGRPAKASAKRAARQQRDAERASPRPSPMQALQSAARKAA
                     AKKGAASGKPRKTRR"
     gene            complement(6466..7962)
                     /locus_tag="PK242_08755"
     CDS             complement(6466..7962)
                     /locus_tag="PK242_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOZ94148.1"
                     /translation="MDTLQHLMTGFGVALTVNNLLVATIGALVGTIVGMLPGLGPING
                     VAILMPLAFALKLPPETALILLAAVYMGCEFGGRISAILINVPGDAGAIMTTLDGYPM
                     AKKGLGGVALSISAWSSFVGSFIATCGIVLFAPLLARWALAFGPAEYFALMCFAFACI
                     TGLMGDAPVKAALAAVIGLSLASVGLDSNSGVYRFTGGDVHLSDGIQFIVLVIGLFSI
                     SEILLMLEQHHSTAGIIKATGRSMFNLKEMAHTWWSTIRSSVVGFVVGVLPGAGATIA
                     SAITYTMEKRLAGPDNRFGDGDIRGVAAPEAANNASAAGSFVPMLTLGVPGSGTTAVM
                     MGALALYNITPGPALFTQNPQLVWGLIASFFVANFLLLLINIPLVGVFARMLQTPNWL
                     LVPAILSVSAVGVYSVHATTFDLVLMSVFGVVGYLLRKQGVPMAPLILGFVLGDMMEQ
                     NLRRALSITDGDVRILAESPISIGLWVLSALMLVVPPLMRLRARAKAR"
     gene            complement(7966..8412)
                     /locus_tag="PK242_08760"
     CDS             complement(7966..8412)
                     /locus_tag="PK242_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOZ94149.1"
                     /translation="MSDRVLGVACVVVAAGMAWAAKDYAAPISYEPVGPRAFPLLLAT
                     LMGIGGLWLIVRPSLRGNWLRQVPLQSLAFAVAAVFAYALLFETLGFTLATTVMAVPV
                     GMAFGGSWKQALAGGLGLGLLLFLGFDKLLDVVLPTGVLSFIFGGR"
     gene            complement(8465..>8790)
                     /locus_tag="PK242_08765"
     CDS             complement(8465..>8790)
                     /locus_tag="PK242_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ94150.1"
                     /translation="IRVLAVMADKRLEGGLANVPTAKEQGLDVEWPIIRGYYMGPKVS
                     DADFKVWTDTFNKMMTTPEFAKLRAERSLFPFAKTGADLDAYIKQRVADYRKLAADFG
                     LNVAK"
BASE COUNT         1572 a         3018 c         2977 g         1223 t
ORIGIN      
        1 ggcatcggcc gctggacggc cgagatgttc ctgatgttcc acctgctgcg ccccgacgtg
       61 ctgccgctgg acgacgtggg cctgatcaac ggcatcagcc tcaactattt ctcgggcgag
      121 cccgtcagcc gcagcgaggc gcgcgaagtg gccgccgcct gggcgccctg gcgcacggtg
      181 gccacgtggt acatgtggcg ctcgctggaa ccccttccgg tcgcctactg aggcggccgc
      241 atccggtaac atcgccggat gtttcgatac cgaggagcca atcttggcca aacgcgcatt
      301 tcttgatttc gagcagccga tcgccgagct tgaaaacaag atcgaggagc tgcgttacgt
      361 gcagagcgag agcgcggtcg acatctcgga ggagatcgac cagctcgaca agaagagccg
      421 tcagctcacc aaggacatct acgccgatct gacgccctgg cagatcacca agatcgcgcg
      481 ccatcccgag cgcccgtaca cgctggacta cgtgcgcgag tgcttcaccg acttcgtcga
      541 gctgcacggc gaccgccatt acgccgacga ccagagcatc gtcggcggcc tggcgcgctt
      601 tggcggcaac gcctgcctgg tgatcggcca ccagaagggg cgcgacacca aggagcgcgc
      661 ggcgcgcaac ttcggcatgg tcaggcccga gggctaccgc aaggcgctgc gcctgatgaa
      721 gacggccgag aagttcaagc tgccggtgtt caccttcgtc gacacgccgg gggcctttcc
      781 gggcatcgac gccgaggagc gcggccagtc cgaggcgatc gggcgcaaca tctacgagat
      841 ggcgcagctg gaggtgccga tcatcagcac catcatcggc gaaggcggct cgggcggggc
      901 gctggccatc gccgtggccg accaggtgct gatgctgcaa tattcgatct attcggtgat
      961 cagccccgag ggttgcgcct cgatcctgtg gaagaccagc gacaaggcgc aggaagcggc
     1021 cgaggcgctg ggcatcacgg cgcaccgcct gaaggcgctg ggcatggtcg acaagatcgt
     1081 cagcgagccg gtcggcggcg cgcaccgcga cccgcgccac atgggcacgc tgctcaagcg
     1141 cgcgctgggc gacgcctggc gccaggtgag cgacctgaag gtcaaggagc tgctggaccg
     1201 ccgctacgag cgcctgatga gctacgggcg cttcaacgac accaaggagc ggtagcccat
     1261 cctgagccgc ggtcgcggcc tcccccgggg ggcaaggcca gtgcccggcg caggtccggg
     1321 cacggcgttc gctgaattgg cctgctgcgc ggcccttcga tgcggtcagc cgcgcgccgg
     1381 ggtgacgcgc gattgcacct tggcgcgcag cgcggtcatc agttcgggcg gcaggaagcg
     1441 gaaggccagg cgcagcagca gccaggcgat caggccgtca tccagccagc ccaggatggg
     1501 gatgaagtcc gacaccaggt ccaccgggct cagcacgtac agcagcgcca gcaccgtcgc
     1561 cagcttggac gccagcggcg cgcgcgggtc gcgcagcaga ccccaggcga gcagcagttc
     1621 cttgcgcagc agcgcgatca ggcgaccgag tttccacatg tgacgactcc cttcgggcaa
     1681 cggcgcgcca tcgtagcagg gtgcgtgccg ccccgccgcg catcgggcgg gctgccgggt
     1741 atcagggaaa gccaagggcg gcgcctggcc aatggcacct acaatatgtt gcagtgcagc
     1801 agtttgaccg accttccatt tcatgagccg tgcccccgcc ccggcgccgg ccaagccggc
     1861 gcgcaagccg tcgaccagcg ccccgtccca gcgggtgatc aagaagtacc ccaaccggcg
     1921 cctgtacgac accgacacct cgacctacat cacgctggcg caggtgcgcg agctggtgat
     1981 ggcgcgccag cccttcgtgg tgcgcgacgc caagaccggc gacgaactga cgcgcagcat
     2041 cctgctgcag atcatcctgg aagaagaagc cggcggcgcg cccatgttca cccaggacat
     2101 gctggccaac atcatccgct tctacggcca tgccatgcag ggcttcatgg gcagctacct
     2161 ggagaagaac atccaggcct tcatggaaat ccaggccaag ctggccgagc agtcctcggg
     2221 gctgaacccc gagctgtggg cgcagttcat gcacatgcag tcgcccatga tgcagggcat
     2281 gatgggcaac tacctggagc agtccaagaa catgttcctg caggcgcagg agcagatgag
     2341 caagcagacc gagcagatgc tgggcgcctt cggcatcaag cgcggcgctt gaggacgcgg
     2401 ccggcgcgcg gggctgcgac aatcgcccca tgagtgaaac caccacgccg ccgcgcatcg
     2461 gcttcgtgtc cctgggctgc cccaaggcgc tgaccgactc cgagctgatc ctgacccagc
     2521 tgtcggccga gggctaccag accagcaaga ccttcgaagg tgccgatctg gtcatcgtca
     2581 acacctgcgg gttcatcgac gaggcggtca aggaaagcct ggacaccatc ggcgaggccc
     2641 tgacggtcaa cggcaaggtc atcgtcaccg gctgtcttgg tgcaaaaacc atagctgctg
     2701 gcgctgatgg gacgggcgcc aaaggtcaaa aagatactga aaacctggtc aaacacctgc
     2761 acccctcggt gcttgccgtc accggcccgc acgccacgca ggaagtgatg gacgcggtgc
     2821 accgccacct gcccaagccg cacgacccgt tcctcgacct ggtgcccggc gcctttggcg
     2881 aggccggcct gaagctcacg ccgcgccact acgcctacct gaagatcagc gagggctgca
     2941 accaccgctg caccttctgc atcatcccct cgatgcgggg cgacctggtc agccgcccga
     3001 tcggcgaggt gctgaaggaa gcacgcgcgc tgttcgaggg cggcgtcaag gaactgctca
     3061 tcatcagtca ggacacctcg gcctacggcg tcgatgtgaa gtaccgcacc ggcttctggg
     3121 acggccgccc gctcaagacc cggctgctgg agctggtgca ggcgctgggc gagctggccg
     3181 cgccgcacgg cgcctgggtg cgcttgcatt acgtgtaccc gtacccacat gtggacgaca
     3241 tcctgccgct gatggccgag ggcaaggtgc tgccgtacct ggatgtgccc ctgcagcaca
     3301 gccacccgga cgtgctgcgc cgcatgaagc ggccggccag cggcgagaag aacctggagc
     3361 gcatcgcgcg ctggcgcgac atttgtcccg agctggtgat ccgctccacc ttcatcgccg
     3421 gttttccggg cgagaccgag gccgagttcg agcacctgct ggacttcatg cgcgaggccc
     3481 gcatcgaccg cgccggctgc tttgcctatt cgccggtcga aggggccgcc gccaacgcgc
     3541 tgcccggcat gctgccgccc gagctgcgcg aagagcgccg cgcgcgcttc atggccgtgg
     3601 ccgaggccgt gtccaccgcc aagctgcgcg agcgcgtggg cgacaccctg caggtgctga
     3661 tcgactcggc gccggcgctg ggccggcgcg gcggggtggg gcgcagctat gccgacgcgc
     3721 ccgagatcga cggcgtggtg catttgctgc cgccgcaaaa aatcagcaag cagctgcggg
     3781 tgggcgagtt cacgcgggcg cgggtggtcg acacccaggg ccacgacctg atcgcccagc
     3841 ccctctgagc cggatgaaaa ctgatgcgta cgcgcaacag ctggctcggg cgggcaagaa
     3901 aaaagccacc cgatgggtgg cttgtcatcg attgcagcgg tgttggcggc tgtaatcttc
     3961 ttttgttttg gtgcccagga gaggactcga acctccacgc ctctcagcgc tagtacctga
     4021 aactagtgcg tctaccaatt ccgccacctg ggcatttcag gaaagctagg attctatatc
     4081 aaaaatctcg actccaccag cagcctgctg gaagaaattg aaggcacggt gcaaggccac
     4141 cgcgacgggc acggtttcgt gctgcgcgac gacggcgacg ccgacatcta tctgcccccc
     4201 aacgagatgc gcgcggtgct gcaccgcgac cgcgtgcgcg tgcgcatcgt gcggcaggac
     4261 cggcgcgggc gccccgaggg gcgcgtgctc gaaatcgtcg agcgcccgcc gcaacccatc
     4321 atcggtcgcc tgctgcagga aggcggcatc tggctggtgg ccccggaaga ccggcgctat
     4381 ggccaggacg tgctgattcc caagaccgcc atcggtctgg cccggccggg tcaggtggtg
     4441 gtggtcgagc tgaccgagcc gcccgcgctc tatggccagc cggtcgggcg cgtcaaggaa
     4501 gtgctgggcg acatcgatga ccccggcatg gagatcgaaa tcgcggtgcg caagtacggc
     4561 gtgccgcacg agttctccga cgccgccatc ggcgaggcgc gcgccctgcc cgacagcgtg
     4621 cagccgcgcg accgcgccgg ccgcgtcgac ctgaccgacg tgccgctggt caccatcgac
     4681 ggcgaggacg cgcgcgactt cgacgacgcg gtgtactgcc agcccgccgt ggtcaccggc
     4741 cgcggcaaga aacccaacgg ctggcgcctg ctggtggcga ttgccgacgt cagccactac
     4801 gtgcgcaacg gcgcgccgct ggacgtggac gcctacgacc gcgccaccag cgtgtacttt
     4861 ccgcgccgcg tcatccccat gctgccggaa aagctcagca acggcctgtg ctcgctcaac
     4921 cccgaggtcg agcgcctgag catggtgtgc gacatgctgg tcacggcgca gggtgacgtc
     4981 catgcctacc agttctaccc ggccgtcatc cgcagccatg cgcggctgac ctacaccgag
     5041 gtggcggcca tcctggccaa cacgcgcggc cccgaggcgg cgcgccggca ggcgcgcgtg
     5101 cagaacctgc tggacctgca cgacgcgttc cgcgccttgc tgaaggcgcg ccagcggcgc
     5161 ggcgcggtgg atttcgacac cgtggagaca cagatcgttt gcgacgaggc cggccggatc
     5221 gagaagatcg tgccgcgcca gcgcaacgac gcgcaccgcc tgatcgagga gtgcatgctg
     5281 gcggccaacg tgtgcagcgc cgacttcatc ggccagggca agcacgccgg cctgtaccgc
     5341 gtgcacgagg gcccgacgcc agaaaagctc gagctgctgc gcgcctacct gaaggcgctg
     5401 ggcctgggcc tgtcggtcag cgacgagccg cggccggacg agttccagcg catcgccgcg
     5461 gccaccgccg agcgtcccga cgccgagcag atccacatga tgctgctgcg cgccatgagc
     5521 caggccatct actcgcccgt caacagcggg cacttcggcc tggcctacga ggcgtacacg
     5581 catttcacca gcccgatccg gcgctacccg gacctgctgg tgcaccgcgt catcaaggcc
     5641 atcctggccg agcgcaagta ccagctgccg agtctgccca cgcccggcga ggcgcacgaa
     5701 aagctcagca agcgcttgaa aaaagatagc ttgtcgcgca agcgggaaca gcgcgagcgg
     5761 cccgatcgcc tgtcacccga ggtgcaggcc tgggaagccg ccggcctgca ctgcagcgcc
     5821 aacgagcggc gcgccgacga ggccagccgc gacgtcgagg cctggctcaa gtgcaagtac
     5881 atgaaggagc acctgggcga ggaatacgcc ggcaccgtca gcgccgccac cagcttcggc
     5941 ctgttcgtca cgctcagcga gctgcacgtc gagggcctgg tgcacatcac cgaactgggc
     6001 ggcgagtact tccgcttcga cgaagtccgc caggagctgc gcggcgagcg caccggcatc
     6061 cgctacgcca tcggcacgcg ggtgcgcgtg caggtcagcc gcgtcgacct ggacgcgcgc
     6121 aagatcgact tccggctggt gcgcgaggac gaagacctgt tggcgcgcgc cccgcgcgac
     6181 aagggcgcgg cgcccgccgc cgagcgcggc cgcccggcca aggcctcggc caagcgcgcc
     6241 gcgcgccagc agcgcgacgc cgagcgcgcc agccccaggc cctcgccgat gcaggcgctc
     6301 cagtcggccg cgcgcaaggc ggcggccaag aagggcgccg ccagcggcaa gccgcgcaag
     6361 acgcgacgct gagcgcggaa gacaaaaaag ccgctctgaa attcagagcg gcttgcgcag
     6421 gctggtcggg cgctggagcc cgatctgcat gcgatctggc ttgcctcagc gcgccttggc
     6481 gcgtgcgcgc aggcgcatca gcggcggcac caccagcatc agcgcgctca gcacccacag
     6541 gccgatggag atcgggcttt ccgccaggat gcggacgtcg ccgtcggtga tcgacagcgc
     6601 gcggcgcagg ttctgctcca tcatgtcgcc cagcacgaag cccaggatca gcggcgccat
     6661 cggcacgccc tgcttgcgca gcaggtagcc caccacgccg aacacggaca tcagcaccag
     6721 gtcgaaggtg gtggcgtgca ccgagtacac ccccaccgcg ctgaccgaca ggatggccgg
     6781 caccagcagc cagttgggcg tctgcagcat gcgggcgaac acgccgacca gcggaatgtt
     6841 gatcaacagc agcaggaagt tggcgacgaa gaacgaggcg atcaggcccc aaaccagttg
     6901 cgggttttgc gtgaacagcg ccggacccgg cgtgatgttg tacagcgcca gcgcgcccat
     6961 catcaccgcc gtggtgcccg agccgggcac gcccagtgtg agcatgggca cgaaggagcc
     7021 ggccgccgag gcgttgttgg ccgcctccgg cgccgccacg ccgcggatgt cgccgtcgcc
     7081 gaagcggttg tccggcccgg ccaggcgctt ttccatggtg taggtgatgg cgctggcgat
     7141 ggtggcgccg gcgcccggca gcacgccgac cacgaacccc accaccgacg agcggatggt
     7201 ggaccaccag gtgtgggcca tttccttgag gttgaacatc gagcgcccgg tggccttgat
     7261 gatgccggcc gtgctgtggt gctgctccag catcagcagg atctcgctga tcgagaacag
     7321 gccgatcacc agcacgatga actggatgcc gtccgacagg tgcacgtcgc cgccggtgaa
     7381 gcggtagacg ccggagttgg agtccagccc cacggaggcc agcgacaggc cgatcaccgc
     7441 cgccagcgcc gccttgaccg gtgcgtcacc catcaggccg gtgatgcagg cgaaggcgaa
     7501 gcacatcagc gcgaagtact cggccggccc gaaggccagc gcccagcgcg ccagcagcgg
     7561 ggcgaacagc acgatgccgc aggtggcgat gaacgagccg acgaacgagc tccaggccga
     7621 gatcgacagc gccacgccgc ccaggccttt cttggccatg gggtagccgt ccagcgtcgt
     7681 catgatggcg ccggcgtcgc ccggtacgtt gatcaggatg gccgagatgc gcccgccgaa
     7741 ctcgcagccc atgtagaccg cggccagcag gatcagcgcc gtctcgggcg gcagcttgag
     7801 cgcgaaggcc aggggcatca gaatcgccac gccgttgatc ggccccagcc ccggcagcat
     7861 gccgacgatg gtgccgacca gcgcgccgat ggtggccacc agcaggttgt tgacggtcag
     7921 ggcgacgcca aagccggtca tcaggtgttg cagcgtgtcc atggctcagc gccctccgaa
     7981 gatgaacgac agcacgccgg tgggcagcac cacgtccagc agcttgtcaa accccaggaa
     8041 cagcagcagg cccaggccca ggccaccggc cagtgcctgc ttccagctgc cgccgaaggc
     8101 catgccgacc ggcaccgcca tgacggtggt ggccagggtg aagcccagcg tctcgaacag
     8161 cagcgcgtag gcgaacaccg ccgccacggc gaaggccagc gactgcagtg gcacctggcg
     8221 cagccagttg ccgcgcaggc tggggcgcac gatcagccac aggccgccga tgcccatcag
     8281 ggtggccagc agcagcggaa acgcgcgcgg gccgacgggc tcgtaggaaa tcggcgcggc
     8341 gtagtccttg gcggcccagg ccatgcctgc ggccaccacg acacacgcga cgccaagaac
     8401 gcgatcgctc atggtgtgtt gctcctccgg ttcggcgcgg cccgggcacg gtgccgcgac
     8461 agggtcactt ggcgacgttc aggccgaaat cggccgccag cttgcggtag tcggccacgc
     8521 gctgcttgat gtaggcgtcc aggtcggcgc cggtcttggc gaacgggaac aggctgcgct
     8581 cggcgcgcag cttggcgaac tcgggcgtgg tcatcatctt gttgaaggtg tcggtccaga
     8641 ccttgaagtc ggcgtcactg accttcgggc ccatgtagta gccgcggatg atcggccatt
     8701 cgacatccag cccctgctcc ttggcggtgg gtacgttggc caggccgcct tccaggcgct
     8761 tgtcggccat caccgccagc acgcggatct
//
LOCUS       DAOOOG010000107         8733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_9432_length_8733_cov_23.848813, whole genome shotgun sequence.
ACCESSION   DAOOOG010000107 DAOOOG010000000
VERSION     DAOOOG010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9432_length_8733_cov_23.848813"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /gene="rimP"
                     /locus_tag="PK242_08770"
     CDS             <1..552
                     /gene="rimP"
                     /locus_tag="PK242_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOZ94151.1"
                     /translation="TVRGLGLELVELERSAGGLLRVTVDFPWSPGDATERWVTVEDCE
                     RVTRQLQYVLEVEGVDYKRLEVSSPGIDRPLRHAQDFERFAGEVVDLVLKAPIGAAAA
                     GQVAVNRKKFRGTLHRAEGGGWQIVWRDEPVVKPGQRVSARRAPAPEQVLGFELDELQ
                     SARLAPVVDFKGRRRQPATDDGV"
     gene            613..2097
                     /gene="nusA"
                     /locus_tag="PK242_08775"
     CDS             613..2097
                     /gene="nusA"
                     /locus_tag="PK242_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOZ94152.1"
                     /translation="MNRELLMLVEAISREKNVERDVVFGAVEAALAQAAKKLYEGEVD
                     LRVAIDRDTGNYETFRRWHVVPDEAGLQLPDQEILLFEAKEQIPDIEVDEYIEEPVES
                     VPIGRIGAMAAKQVILQKIRDAEREMLLNDFMSRGEKIFTGTVKRMDKGDIIVESGRV
                     EGRLRRSEMIPKENLRNGDRVRAMIMEVDLTLRGAPILLSRSAPEFMIELFRNEVPEI
                     EQGLLEIKSCARDPGSRAKIAVVSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIV
                     LWSDDPAQFVIGALAPANVSSIVVDEEKHAMDVVVDEENLAIAIGRGGQNVRLASELT
                     GWKINIMDADESAQKEAEETGTLRTLFMEKLDVDQEIADILIEEGFTNLEEVAYVPIQ
                     EMLDIEAFDEDTVNELRARAKDALLTQAIAREESVESVSQDLRELAGLTPELIALLAD
                     AGVKTRDDLADLAVDELTAITGQSEEDARALILKAREHWFVDQE"
     gene            2122..4917
                     /gene="infB"
                     /locus_tag="PK242_08780"
     CDS             2122..4917
                     /gene="infB"
                     /locus_tag="PK242_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOZ94153.1"
                     /translation="MTTITVSDFANELKRPADALLEQLQAAGVAKKSVSDALTDSDKQ
                     QLLNYLQASHGTGATRKKITLTKKTTSEIKQADATGKARTIQVEVRKKRTFIKRDEDA
                     APAPAPAVEPPAPAPAPAPAMPVVDAAEAARREEEARRQAELLRRQEEDAAERRRLRE
                     QAERETSEARAAAAEAPSIGAQAEAPAAAAPAPAPAPAMPPAPTAAPAPAPAAEAPST
                     AEDEAARAREQEESRRKALAEAEAIRAMMSAPRKVLVAKRAEDKKPEAKAAPQKAGVA
                     KPAAGPAAAPKEVKSAKLSSSWAGDPAKKKGIPTRGDTSAGRAGWRSGPRGGRRGERG
                     REEQVVAAPVEQRVLEVHVPETITVAELAHKMAVKASEVIKTLMKMGQMVTINQPLDQ
                     DTAMIVVEEMGHTAMVAALDDPEAFTDQEVQAQDAEALPRPPVVTVMGHVDHGKTSLL
                     DYIRRSKVAAGEAGGITQHIGAYHVETPRGVISFLDTPGHEAFTAMRARGAQATDIVI
                     LVVAADDGVMPQTREAIKHAKAAEVPIVVAITKSDKPEANPERIKAELVGEEVVPEEF
                     GGESPFISVSSKTGQGMDELLENVLLQAEVLELKAPVDAAAKGLVIEASLDKGRGPVA
                     TVLVQSGTLKVGDVVLAGQTSGRVRAMLDEDGRSTKTAGPSIPVEIQGLSEVPQAGDD
                     FMVLADERRAREIATYRAGKFRNTKLAKQQAAKLENVFADMQAGEVKTLPLIIKADVQ
                     GSQEALSASLLKLSTDEVKVQMIYSGVGGISESDINLAIASKAIVIGFNTRADAGARK
                     LAEANDVQIRYYNIIYDAVDELKAAMSGMLAPEHKEEVIGMAEIRTVFVASKIGTVAG
                     SYVTSGVVRRDAHFRLLRDNVVIYTGELESLKRLKDDVREVKEGFECGIKLKNYNDIK
                     EGDQLEFFEIKEIARTL"
     gene            5054..5431
                     /gene="rbfA"
                     /locus_tag="PK242_08785"
     CDS             5054..5431
                     /gene="rbfA"
                     /locus_tag="PK242_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOZ94154.1"
                     /translation="MPRRSSKPNRGYQIADQIQRDLAELIARELKDPRVGMVTLNAVE
                     VTPDYAHAKVFFSLLTGDPQETEEALNHAAGHLRNGLFKRLHIHTVPTLHFVFDRTTE
                     RAADMNALIARAVASRAKDEDPT"
     gene            5428..6381
                     /gene="truB"
                     /locus_tag="PK242_08790"
     CDS             5428..6381
                     /gene="truB"
                     /locus_tag="PK242_08790"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOZ94155.1"
                     /translation="MTAAPRTRVQRRPVHGVLLLDKPLGLSSNQALQKVKWLLRAEKA
                     GHTGTLDPLASGVLPLCFGAATKFSALHLDADKRYHATLQLGVRTRTGDREGEVLACR
                     PVPPLSAALLEAVRQRFLGRILQLPPMHSALKKEGRALYEYARAGVEVERTPRAVRIH
                     ELILSLAPAEQAPTAIEMIVFCSKGTYIRTLGEDIGEALGCGGHLSALRRVATGPFDQ
                     HQCVTLEQLEAMDEPARLACLRPPEVLLADHLPITLPAEEAGRFLSGMRRRGPWPDAD
                     RVAVFGEQPRALLGTAHVRAGELIPGRLLSPIELKQMLHLT"
     gene            6378..8630
                     /locus_tag="PK242_08795"
     CDS             6378..8630
                     /locus_tag="PK242_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ94156.1"
                     /translation="MTTAPSSAPDTAAAALTRAAQAWQAGAWGQVIALLTPWVDATPP
                     APEALQLIAMAHLKLGQMAPALDRQRQLTRLQPGSAVVWINLAGTEAALGQTRQALAS
                     LDRALVLDPLQPAAHFNRANALMQLGEAAAACDGYRRAAELAPDRPDPPYNLAMALLQ
                     LDRHEEALGIMRAVVERHPALAPAWNVLGMAWHRLNADQQALDSYQQAIRLDPGLADA
                     WSNGAQVLARLERRDEAQTWARQAVQLNPQHAAALRTLGVVLASAQAPQQKEARLWLE
                     KALELDPRDTTALSTLVDLDTLHCDWSSLDAHLSRLRTAWHDSAPEGLELWRLLSLPI
                     SGHELRALTQATCARRWGKTAAQSAALPRRHVGQPRPQRLRIGYFSCDFRQHATSVLT
                     AGMFEAHDRSRFETVAYCLALNPPDASDPMRQRLRSAFERWESVGRLTDAELVRLARE
                     HDIHIAVDLQGHTAGARMGVFAARVAPIQLHYLAYAGTLGMPGSIDYLVTDPVVVPPA
                     ARPHFSEKLIELPDSYQVNDRARLIDPLRPSRAELGLPANGFVFCCFNNNYKIRRDVF
                     AIWMSVLRERPGSVLWLLADHAETVQNLRAAAEGHGIDPNRLVFAERTSLPRHLARQA
                     QADLFLDTWPYSAHTTASDALWAGLPLVTCAGETFASRVGASLLRACGLPQLIAHDPQ
                     AYREKILALSAAPEPLQAIRRQLLETRLSVPLFDTERFTRHIERAYDMAWERHMQGLA
                     PDHLTVPPLP"
BASE COUNT         1571 a         2901 c         3010 g         1251 t
ORIGIN      
        1 accgtgcggg gcctgggcct cgaactggtg gaactggagc gttccgccgg cgggctgctg
       61 cgcgtcacgg tggactttcc ctggtcgccc ggcgatgcca ccgagcgctg ggtcacggtg
      121 gaagactgtg agcgcgtgac gcgccagttg cagtacgtgc tggaagtcga aggcgtggac
      181 tacaagcggc tggaagtatc gtcgcccggc atcgaccggc ccctgcgtca cgcgcaggat
      241 ttcgagcgtt ttgccggcga ggtggtcgat ctggtgctga aggcgcccat cggagcggcg
      301 gcggcagggc aggtggcggt caaccgcaag aagttccgcg gcaccttgca cagggccgag
      361 ggcggcggct ggcagatcgt ctggcgcgac gagccggtgg tcaagccggg tcagcgcgtc
      421 agcgcccggc gggcgcccgc gcccgagcag gtgctgggct tcgagctcga tgagttgcag
      481 tcggcgcggc tggcgccggt ggtggatttc aagggtcgcc ggcgccagcc ggcgacggac
      541 gacggggttt gagaagcgga acccggcgcc caaggcgcgt gcggtccgca gacatgggaa
      601 caggtgagaa tcatgaatcg cgaactgttg atgttggttg aggcgatttc gcgcgaaaag
      661 aacgtcgagc gtgacgtggt gttcggcgcc gtcgaggcgg cgctggcgca ggcggccaag
      721 aagctgtatg agggcgaagt cgatctgcgt gtcgcgatcg atcgcgacac gggcaactac
      781 gaaaccttcc gccgctggca cgtggtgccc gatgaggccg gcctgcagtt gcccgaccag
      841 gaaatcctgc tgttcgaggc caaggagcag attcccgaca tcgaggtcga cgagtacatc
      901 gaggagccgg tcgagtcggt gcccatcggc cgcatcggcg ccatggcggc caagcaggtg
      961 atcctgcaga aaatccgcga cgccgagcgc gagatgctgc tcaacgactt catgagccgc
     1021 ggcgaaaaaa tcttcaccgg caccgtcaag cgcatggaca agggcgacat catcgtcgag
     1081 tccggccgcg tcgaaggtcg cctgcgccgc agcgaaatga tcccgaagga aaacctgcgc
     1141 aacggcgacc gtgtgcgcgc catgatcatg gaggtcgatc tgaccctgcg cggcgccccg
     1201 atcctgctgt cgcgctcggc gcccgagttc atgatcgagc tgttccgcaa cgaggtgccc
     1261 gagatcgagc agggcctgct ggagatcaag agctgcgcgc gcgatccagg cagccgcgcc
     1321 aagatcgccg tcgtcagcca cgacaagcgc gtggacccga tcggtacctg cgtcggagtg
     1381 cgcggcacgc gcgtcaacgc cgtcaccaac gagctggcgg gcgagcgcgt ggacatcgtg
     1441 ctgtggtcgg acgatccggc gcagttcgtg atcggcgcgc tggcgccggc caacgtgtcc
     1501 tcgatcgtgg tcgacgagga aaagcacgcc atggatgtgg tggtcgacga ggagaacctg
     1561 gccatcgcca tcggtcgtgg cggacagaac gtgcgcctgg cctccgagct gacgggctgg
     1621 aagatcaaca tcatggacgc cgacgagtcg gcgcagaagg aggccgagga aaccggtacc
     1681 ctgcgcacgc tgttcatgga aaagctcgac gtcgaccagg agatcgccga catcctgatc
     1741 gaggaaggct tcaccaacct cgaggaagtg gcctacgtgc cgatccagga aatgctggac
     1801 atcgaggcgt tcgatgaaga caccgtcaac gaactgcgcg cccgcgccaa ggacgcgctg
     1861 ctgacccagg cgattgcgcg cgaagagagc gtggaatcgg tgtcgcagga tctgcgcgag
     1921 ctggccggcc tgacgcccga actgattgcc ctgctggccg acgccggcgt gaaaacccga
     1981 gacgacctgg ccgatctggc cgtagacgaa ttgaccgcga tcacgggtca gtccgaagag
     2041 gacgcccgtg ccctgatcct gaaggcgcgc gagcactggt tcgtggatca agagtaagga
     2101 tcatggaggc ccgcaccgca tatgacgacc atcaccgttt ccgatttcgc gaacgagctc
     2161 aagcgtccag ctgacgcatt gctggaacag ctccaggccg ccggggtggc caagaagtcg
     2221 gtgtccgacg ccctgaccga cagcgacaag cagcagttgc tgaactacct gcaggccagc
     2281 catggcaccg gggcaacccg caagaagatc acgctgacca agaagaccac cagcgagatc
     2341 aagcaggccg acgccaccgg caaggcacgc accatccagg ttgaggtgcg caagaagcgc
     2401 accttcatca agcgcgacga agacgccgcg ccggcgccag caccggcggt cgagcctccg
     2461 gcgcccgcgc cggcacctgc gcccgccatg ccggtggtcg acgccgccga ggccgcccgg
     2521 cgcgaagaag aggcacgccg ccaggccgag ttgctgcgcc gacaggaaga agacgcggcc
     2581 gaacggcgcc gcctgcgcga gcaggcggag cgcgaaacgt ccgaggcacg cgcagcggcg
     2641 gctgaagcgc cgtcgatcgg cgcacaggcc gaggcgccgg cagccgccgc gcctgcgcct
     2701 gcgcctgccc cagccatgcc gcccgcgccg acggccgcac ccgcacccgc acccgctgcg
     2761 gaggcgccca gcactgccga ggacgaggcc gcgcgcgccc gcgaacagga agaaagccgc
     2821 cgcaaggccc tggccgaggc tgaggccatt cgcgccatga tgagcgcgcc gcgcaaggtg
     2881 ctggtcgcca agcgagccga agacaagaaa cccgaggcca aggcggcacc gcagaaagcc
     2941 ggtgttgcca agccggccgc agggcctgcg gccgcgccga aggaggtcaa gtcggccaag
     3001 ctgtcgtcca gctgggccgg tgatccggcc aagaagaagg gcattccgac ccggggcgat
     3061 accagcgctg ggcgtgccgg ctggcgcagc ggcccgcgcg gcgggcgccg tggcgagcgt
     3121 gggcgcgagg agcaggtggt tgccgcaccg gtggaacaac gggtgctgga agtgcacgtg
     3181 cccgaaacca tcaccgtggc cgagctggcg cacaagatgg cggtcaaggc ctccgaagtc
     3241 atcaagacgc tgatgaagat gggccagatg gtcaccatca accagccgct ggaccaggac
     3301 acggcgatga tcgtggtcga ggaaatgggc cacaccgcca tggtggcggc gctcgacgat
     3361 ccggaggcct tcaccgacca ggaagtgcag gcgcaggacg ccgaggcgct gccgcgcccg
     3421 ccggtggtga ccgtgatggg tcacgtggac cacggcaaga cctcgctgct ggactacatc
     3481 cgccgcagca aggtggccgc tggcgaagcc ggcggcatca cgcagcacat cggcgcctac
     3541 catgtcgaga ccccgcgcgg cgtgatttcg ttcctcgaca cgccgggcca cgaggcgttc
     3601 accgccatgc gtgcgcgcgg cgcgcaggcc accgacatcg tgatcctggt cgtcgcggcc
     3661 gacgacggcg tgatgccgca gacgcgcgaa gccatcaagc acgccaaggc ggccgaagtg
     3721 ccgatcgtgg tggccatcac caagtccgac aagcccgaag ccaaccccga gcggatcaag
     3781 gccgagctgg tgggcgaaga ggtggtgccc gaggaattcg gtggtgagtc gccgttcatc
     3841 tcggtgtcga gcaagaccgg tcagggcatg gacgagctgc tggagaacgt gctgctgcag
     3901 gccgaagtgc tggaactgaa ggcgccggtc gatgccgccg ccaagggcct ggtcatcgaa
     3961 gccagcctgg acaagggccg cggcccggtg gccacggtgc tggtgcagtc gggcacgctg
     4021 aaggtgggcg acgtggtgct ggccggtcag acctccggcc gcgtgcgcgc catgctggac
     4081 gaggacgggc gctccaccaa gacggccggc ccgtcgattc cggtggaaat ccagggcctg
     4141 tccgaagtgc cgcaggccgg cgatgacttc atggtgctgg ccgacgagcg gcgcgcgcgc
     4201 gaaatcgcca cctaccgcgc cggcaagttc cgcaacacca agctggccaa gcaacaggcg
     4261 gccaagctgg agaacgtgtt cgccgacatg caggccggcg aggtcaagac gctgccgctg
     4321 atcatcaagg ccgacgtgca aggctcgcag gaggcgttgt cggcttcgct gctcaagctc
     4381 agcaccgacg aggtgaaggt gcagatgatc tacagcggtg tgggcggcat cagcgagagc
     4441 gacatcaacc tggccatcgc ctccaaggcc atcgtgatcg gcttcaacac ccgtgccgac
     4501 gccggggcgc gcaagctggc cgaggccaac gacgtgcaga tccgctacta caacatcatc
     4561 tacgacgccg tggacgagct gaaggcggcc atgagcggca tgctggcgcc ggagcacaag
     4621 gaagaagtca tcggcatggc cgagatccgc accgtgttcg tggccagcaa gatcggcacc
     4681 gtggcgggct cctacgtcac cagcggcgtg gtgcggcgcg acgcccattt ccgtctgctg
     4741 cgcgacaacg tggtgatcta caccggcgag ctggaatcgc tcaagcgcct gaaggacgac
     4801 gtgcgtgaag tcaaggaagg cttcgagtgc ggcatcaagc tgaagaatta caacgacatc
     4861 aaggaaggcg accagctgga gttcttcgag atcaaggaga tcgcgcgtac gctgtgaggc
     4921 ttgtcggccc acaatgaaca gccgtacgca gagaacgcag agattgcgca aagggcgccg
     4981 agaaagcagg acgatgccct ctggcgtcct ctgcgcttgc tttgcgtccg ctgcgtacgg
     5041 tggttgaatt tccatgccca gacgcagcag caaacccaat cgcggctatc agatcgccga
     5101 ccagattcag cgcgatctgg ccgagttgat cgcgcgcgag ctgaaggacc cgcgcgtggg
     5161 catggtgacg ctcaatgccg tcgaggtcac tcccgactac gcccacgcca aggtgttctt
     5221 cagtctgctg acgggcgacc cgcaggaaac cgaagaggcg ctcaaccacg ccgccggtca
     5281 tctgcgcaac ggcttgttca agcggctgca catccacacc gtgccgacgc tgcacttcgt
     5341 gttcgaccgc accaccgagc gcgcggccga catgaacgcg ctcatcgcgc gggcagtggc
     5401 ctcgcgcgcc aaggacgaag acccgacatg acggctgccc cacgcaccag ggtgcagcgg
     5461 cgccctgtgc atggggtgct gctgctcgac aagccgctgg gcctgtccag caaccaggcg
     5521 ctgcagaagg tcaagtggtt gctgcgcgcc gagaaggccg gtcacaccgg cacgctcgat
     5581 ccgctggcca gcggtgtgct gcccctgtgt tttggcgctg ccaccaagtt cagcgcgctg
     5641 cacctggacg ccgacaagcg ctaccacgcc accttgcagc tgggcgtgcg cacccgcacc
     5701 ggagaccgtg agggcgaggt gctggcgtgc cggccggtgc cgccactgtc ggcggctttg
     5761 cttgaagcgg tgcgacagcg ctttctgggg cgcatcctgc agctgccgcc catgcacagc
     5821 gcgctcaaga aggagggcag ggcgttgtac gaatacgcgc gtgccggcgt ggaggtggag
     5881 cgcacaccgc gcgcggtccg gatccatgaa ctgattctga gcctggcgcc cgcggagcaa
     5941 gcgccgactg ctatcgaaat gatagtgttc tgcagcaagg gcacctatat ccgcaccctg
     6001 ggcgaagaca tcggcgaggc cctgggttgc ggtggccacc tgagcgcgtt gcgacgggtg
     6061 gcgaccgggc cgttcgacca acaccagtgc gtcaccctgg agcaactgga agcgatggac
     6121 gaaccggcgc ggctggcctg cctgcgcccc cccgaggtgc tgctggccga tcacctgccg
     6181 atcacgctgc ccgcggagga ggcgggacgc tttctgtccg gcatgcgccg acgcggcccc
     6241 tggcccgatg ccgatcgggt ggcggtgttc ggcgaacagc cacgcgcgct gctgggaacc
     6301 gcccatgtgc gcgccggcga gctgatcccc ggccggctgc tgtcgcccat cgagttgaag
     6361 cagatgctcc atctgacatg accaccgcac cgtcctcagc tcccgacacg gccgctgcgg
     6421 ccctgacgcg cgccgcacag gcctggcagg cgggtgcctg gggccaggtg atcgcgctgc
     6481 tgacaccgtg ggtggatgcg acgccgcccg cgcccgaggc gttgcagctg atcgccatgg
     6541 cgcacctgaa actgggacaa atggcgccgg cgctcgaccg gcagcggcag ctgacccggt
     6601 tgcagcccgg cagcgccgtt gtctggatca acctggcagg caccgaggcg gccctcggtc
     6661 agacccggca ggcgctcgcg agtctggatc gcgcgctggt cctcgatccg ctgcagcccg
     6721 cggcccactt caaccgcgcc aatgcactga tgcaactggg cgaagccgca gccgcctgcg
     6781 acggctatcg ccgtgccgcc gagctggccc ccgatcgtcc cgacccgccg tacaacctgg
     6841 ccatggcctt gctgcagctg gatcgtcacg aagaggcgct ggggatcatg cgtgccgtag
     6901 tggaacgcca tcccgctctg gcccccgcgt ggaacgtgct gggcatggcg tggcatcggt
     6961 tgaacgccga tcagcaggcg ctggacagct accagcaggc catacgcctc gatcccgggc
     7021 tggcggatgc ctggagcaac ggggctcagg tgctggcgcg gttggagcgg cgcgatgaag
     7081 cgcagacctg ggcgcgtcag gctgtgcagc tgaaccccca acacgccgcc gcactgcgca
     7141 cgctgggggt ggtgctggcc agcgcccagg cgccccagca gaaggaggca cgactgtggc
     7201 tggagaaggc gcttgagctc gatccgcgcg acaccacggc cctcagcact ctggtcgatc
     7261 tggacaccct gcactgcgac tggagctcgc tcgatgccca cctgtcccga ctgcgcaccg
     7321 cgtggcacga tagtgcgccc gagggccttg agttgtggcg cctgctgagt ctgccgatat
     7381 ccggccatga gctgcgcgcg ctgacgcaag ccacctgcgc gcgccgctgg ggcaaaaccg
     7441 cagcccagtc ggccgcgttg cctcgacggc atgtgggcca gccgcgtccg cagcggctgc
     7501 gcatcgggta cttttcctgc gattttcgtc aacacgccac cagtgtgctg acggccggca
     7561 tgttcgaggc gcatgatcgc tcgcgcttcg aaaccgtggc ctactgcctg gcgctgaacc
     7621 cgcctgacgc cagtgacccc atgcgtcagc gcctgcggtc ggccttcgaa cggtgggagt
     7681 cggtggggcg tctgaccgac gccgagctgg tgcgcctggc gcgtgagcac gacattcaca
     7741 tcgccgtgga cctgcaaggc cataccgcgg gcgcgcgcat gggggttttt gcagctcgcg
     7801 tggcgcccat ccagttgcac tacctggcct atgcgggcac cctgggcatg cccggcagca
     7861 tcgactacct ggtgaccgac ccggtggtcg tgccgcccgc cgctaggccg cacttcagcg
     7921 agaagctcat cgagctgccg gacagctacc aggtcaacga tcgtgcgcgt ctgatcgatc
     7981 ccctgcggcc ctcgcgtgcc gagctgggcc tgcccgccaa cggtttcgtg ttctgctgct
     8041 tcaacaacaa ctacaagatc cggcgcgatg tgtttgccat ctggatgtcg gtgctgcgcg
     8101 agcgccccgg cagtgtgctg tggctgctgg ccgatcacgc cgagacggtg cagaacctgc
     8161 gggcggcggc cgaaggccat ggcatcgacc ccaaccgatt ggtgtttgcg gaacgcacgt
     8221 cgttgccgcg gcatctggcg cgccaggccc aggccgacct gttcctcgac acctggccct
     8281 acagcgcaca caccaccgcc agcgacgcgc tctgggccgg gctgccgctg gtcacctgcg
     8341 ccggcgaaac ctttgcgtcg cgtgtgggcg ccagcctgct gcgcgcctgt gggctgccgc
     8401 agctgatcgc gcacgatccg caggcgtacc gcgaaaagat cctggcgctg tcggccgcgc
     8461 ctgagccact gcaggccatc cgccgtcagt tgctcgaaac ccggctgtcc gtgcccctgt
     8521 tcgacaccga gcgcttcacg cgtcacatcg agagggccta cgacatggcg tgggagcgcc
     8581 acatgcaggg actggccccc gatcacctga ccgtgccacc tctgccctga cccatcgacc
     8641 ctgactgatc tgagttcatc atgacccgac ccattcgcaa catcgccatc attgcccacg
     8701 tcgaccacgg caagaccacc atggtcgacc agc
//
LOCUS       DAOOOG010000108         8720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_9452_length_8720_cov_23.991460, whole genome shotgun sequence.
ACCESSION   DAOOOG010000108 DAOOOG010000000
VERSION     DAOOOG010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9452_length_8720_cov_23.991460"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /gene="typA"
                     /locus_tag="PK242_08800"
     CDS             <1..1787
                     /gene="typA"
                     /locus_tag="PK242_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOZ94157.1"
                     /translation="DHGKTTMVDQLLRQSGTFAEHEKVVDTVMDNHAIERERGITILA
                     KNCAVSWQGTHINIVDTPGHADFGGEVERALSMVDGVLLLIDAQEGPMPQTRFVTKKA
                     LALGLKPIVVVNKVDKPGARPDHVINAAFDLFDKLGANDEQLDFPVVYASGINGWSSL
                     TEGEPGEQWGPDMAPLFETILKHVPPHQGDPNGPLQLQVSALDFSTFVGRIGVGRINA
                     GTLRPGQDVLVMAGPEGASYKARVNEVLTFQGLDRIKVSEAGPGDIVLVNGIEHIGIG
                     ETLTDPADPQPLPMLKIDEPTLTMNFCVNTSPLAGREGKFVTSRQIWDRLQRELRSNV
                     ALRVKETSEDGVFEVSGRGELHLTILLEEMRREGYELAVSKPRVVFKDIDGERCEPVE
                     MVTADIEEGHQGGVMQALGERKGELVNMEPDGRGRVRLEYRIPARGLIGFTNEFLNLT
                     RGSGLISNLFDGYEPHKGEIGGRKNGVLISMDDGEIFTYALGKLDDRGRMFVRAGDPV
                     YEGMIVGVHNRDNDLVVNATRTKQLTNFRVSGKEDAIKITPPILLTLEYGVEFIEDDE
                     LVEITPKSIRLRKRFLKEHERKRASREA"
     gene            1952..2914
                     /locus_tag="PK242_08805"
     CDS             1952..2914
                     /locus_tag="PK242_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ94158.1"
                     /translation="MASNLTAAAPPWSHGRAAWTMVLTTLLWSSAGVVTRQLQVAQGF
                     EVTFWRSFFTVLSLLLILPLWQGPGVFARMPWRRGTFWLSGLCWAVMFTAFMVALTLT
                     GVARVLVTMALGPLLTALLARVVTGHRLPPRTWAAIVLGGLGMAWMFAGQAGAADAPG
                     AWLGSLVALGVPAAAAVNWTVVQRSQAHGEPIDLVPSVLLGAVISSAITLPLAWPLTA
                     SPHDLGWLAFLGLTQLAIPCVLAVLCARVLPAAEVALLGLLEIVFGILLVWWIVGEAP
                     RPEVLSGGALVMGALLGNELLGWYAARRAAGAGAGSDIAPLGPA"
     gene            complement(3287..3766)
                     /gene="smpB"
                     /locus_tag="PK242_08810"
     CDS             complement(3287..3766)
                     /gene="smpB"
                     /locus_tag="PK242_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOZ94159.1"
                     /translation="MATKKPKTSNPVIAENKKAAFNYFFEERFEAGMVLEGWEVKALR
                     AGKAQLTDGYVVIRAGELFVIGCQINPLGTASTHVRPDAVRTKKLLLHAEQIQRLIGK
                     VEQKGYTLVPLKLYWKNGRVKCEIALAKGKATHDKRDTIKEREGKREVERALKQRHR"
     gene            4017..4469
                     /locus_tag="PK242_08815"
     CDS             4017..4469
                     /locus_tag="PK242_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HOZ94160.1"
                     /translation="MKSVHKSVLIWYSAQEMFDLVVDVEHYPAFLPWCSHGKVMSHEE
                     GGMTAEVGIDFKGVRQTFVTRNTHVTGQEVHLHLVKGPFSRLEGRWVFTPVGQGGQRA
                     CRVDLRLNYGFSNAVLAKVVGPVFDRIASSLMDAFIKRAEQVYGAAAA"
     gene            4466..4837
                     /locus_tag="PK242_08820"
     CDS             4466..4837
                     /locus_tag="PK242_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HOZ94161.1"
                     /translation="MSPPPRPAACATLLEIELTYSPAPRQLVQRRLRLPGGSTVGDAL
                     KAAGLASPVTFAGGQVPWAVGVWGRPAALEQPLCAFDRVELWRPLRVDPKLARRERFG
                     QQGARAAGLFARRRPGAKPGY"
     gene            complement(4951..5484)
                     /locus_tag="PK242_08825"
     CDS             complement(4951..5484)
                     /locus_tag="PK242_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOZ94162.1"
                     /translation="MRTSRILLIGLILAAAAAHAQYQWVDKDGRRVFSDRPPPADVPA
                     KNVLTQPRGARAPQATAAVGPSASASASEPASAAAGAAAARPAASAAGGVDKALEEKK
                     KQNEAAEAARKKAEEDRVAAARAENCKRARSAKATLESGMRMARVNDKGEREVLDDAQ
                     RATELQRVNAIIASDCK"
     gene            5896..7803
                     /gene="thrS"
                     /locus_tag="PK242_08830"
     CDS             5896..7803
                     /gene="thrS"
                     /locus_tag="PK242_08830"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOZ94163.1"
                     /translation="MIHITLPDGSQREFPGPVTVAEVAQSIGAGLAKAALGGKVDGKL
                     VDTSFRIDHDVQLAIITAKDAQGLEMIRHSTAHLLAYAVKELFPEAQVTIGPVIENGF
                     YYDFSYKRPFTPEDLAAIEKKMEQLAAKDEPVLRRVLPRDEAVEYFKGLGEHYKAEII
                     ASIPANEDVSLYREGAFEDLCRGPHVPSTGKLRHFKLMKVAGAYWRGDHRNEMLQRIY
                     GTAWASKDDLKAYLTMLEEAEKRDHRRLGRELDLFHIDEHAPGTVFWHPKGWTVWQEV
                     EQYMRRVYRDNGYHEVKGPQILDKTLWEKTGHWDKYRENMFVTESEKRDYALKPMNCP
                     GHILIYKQGVKSYRDLPLRYGEFGNCHRNEPSGGLHGIMRVRGFTQDDGHIFCTEDMI
                     QAEVAAFTRLLQKVYRDFGFTEIIYKLSTRPAARIGTEESWDKAEAALAEGLRASGCE
                     FSYLPGEGAFYGPKIEYTLKDALGREWQCGTIQVDPNMPERLDAEYVGEDGARHRPIM
                     LHRAIVGSLERFIGILIEQHAGALPVWLAPVQVMVTGITDAQADYARSVAKTLRNQGF
                     RAETDLRNEKITYKIREHSLQKLPYILVVGDKEREAGAVAVRARGNVDLGVMSLEAFA
                     KRIGEDIASKA"
     gene            7889..8494
                     /gene="infC"
                     /locus_tag="PK242_08835"
     CDS             7889..8494
                     /gene="infC"
                     /locus_tag="PK242_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOZ94164.1"
                     /translation="MATNFRDRRQREERAHRLNREIMAPEVRLNGPNNEPLGIVPLQE
                     ALRMAGDLDVDLVEVVATANPPVCRLMDYGKFKYQEQKKAAEAKAKQTVIEIKEVKFR
                     PGTDDGDYQIKMRNIRRFLADGDKVKVTLRFRGREITHQELGLALLDRIRADLADSVV
                     VEQFPKLEGRQMIMMLAPARKKTGGGAATKAAGADQQPAAA"
     gene            8649..>8720
                     /locus_tag="PK242_08840"
     CDS             8649..>8720
                     /locus_tag="PK242_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOZ94165.1"
                     /translation="MPKMKTKSSAKKRFRVRPGGTVKR"
BASE COUNT         1493 a         2836 c         2930 g         1461 t
ORIGIN      
        1 tcgaccacgg caagaccacc atggtcgacc agctgctgcg ccagtccggc acctttgccg
       61 agcatgaaaa ggtggtcgac accgtgatgg acaaccacgc catcgaacgc gaacgcggca
      121 tcaccatcct ggccaagaac tgcgccgtca gctggcaggg cacccacatc aacatcgtcg
      181 acacgcccgg ccatgccgac ttcggcggtg aggtggagcg cgcgctgtcg atggtcgatg
      241 gcgtgctgct gctgatcgac gcgcaggaag gccccatgcc gcagacgcgc tttgtcacca
      301 agaaggcgct ggcgctgggc ctcaagccca tcgtggtggt caacaaggtc gacaagcccg
      361 gcgcgcggcc tgaccacgtg atcaacgccg cgttcgatct gttcgacaag ctgggtgcga
      421 acgacgaaca gctcgacttc ccggtcgtct acgcctccgg catcaatggc tggagctcgc
      481 tcaccgaggg cgagccgggc gaacaatggg ggccggacat ggcgccgctg ttcgagacca
      541 tcctcaaaca cgtgccgccg caccagggcg acccgaacgg cccgctgcag ctgcaggtgt
      601 cggcgctgga cttctcgacc ttcgtcggcc gcatcggcgt cggacgcatc aacgccggca
      661 cgctccggcc cggtcaggac gtgctggtga tggccggccc cgaaggcgcc agctacaagg
      721 cgcgtgtcaa tgaagtgctg accttccagg gcctggatcg catcaaggtc agcgaagccg
      781 ggccgggcga catcgtgctg gtcaacggta tcgagcacat cggcatcggc gaaaccctga
      841 ccgatcccgc cgacccgcag ccgctgccga tgctgaagat cgacgagcct acgctgacca
      901 tgaacttctg cgtcaacacc tcgccgctgg ccgggcgcga gggcaagttc gtcaccagtc
      961 gccagatctg ggaccggctg cagcgcgagc tgcgctccaa cgtcgcactg cgggtgaagg
     1021 aaaccagcga agacggcgtg ttcgaggtct ccggccgcgg cgagctgcac ctgaccatcc
     1081 tgctggaaga aatgcgccgc gaaggctacg aactggccgt atccaagccg cgcgtggtgt
     1141 tcaaggacat cgacggcgag cgctgcgagc cggtggagat ggtcaccgcc gacatcgagg
     1201 agggccatca gggcggcgtg atgcaggcgc tgggcgagcg caagggcgaa ctggtgaaca
     1261 tggagcccga cggccgtggc cgcgtgcgcc tggaataccg cattccggcg cgcggcctga
     1321 tcggtttcac caacgagttt ctgaacctga cgcgcggctc gggcctgatc agcaacctgt
     1381 tcgacggcta tgagccgcac aagggcgaga tcggcgggcg caagaacggt gtgctgatca
     1441 gcatggacga cggcgagatc ttcacctacg ccctgggcaa gctggacgac cgcggccgca
     1501 tgttcgtgcg cgcgggcgat ccggtgtacg agggcatgat cgtgggcgtg cacaaccgcg
     1561 acaacgacct ggtggtcaac gccacgcgca ccaagcagct gaccaacttc cgcgtcagcg
     1621 gcaaggaaga cgcgatcaag atcacgccgc cgattctgct gacgctggaa tacggggtcg
     1681 aattcatcga ggacgatgag ctggtcgaga tcacgcccaa gagcatccgc ctgcgcaagc
     1741 gcttcctgaa ggagcacgaa cgcaagcgcg ccagccgcga ggcctgagca agccacaacc
     1801 aacccctcat gcccgctttg aacgcaccga cacacccgct cggcgttgcc caggctcgcc
     1861 gcagcccgag cgacggctgc gcggtgcacc ctgaacgccc gcgtgggggt atcccccgcg
     1921 ggcgatcggc attcattccc gtcgtccgac catggcgtcc aacctgaccg ctgccgcgcc
     1981 gccctggagc catggccgcg ccgcctggac catggtgctg accacgctgc tgtggtccag
     2041 cgccggcgtg gtgacgcgcc agttgcaggt ggcgcaaggc ttcgaggtaa cgttctggcg
     2101 cagcttcttc accgtgctgt cgctgctgct gatcctgccg ctgtggcagg ggccgggcgt
     2161 gttcgctcgc atgccgtggc ggcgcggcac gttctggttg tcgggcctgt gctgggcggt
     2221 catgttcacc gccttcatgg tggcgctgac cctgaccggg gtggcgcgcg tgctggtcac
     2281 catggcgctg gggccgttgc tgacggcctt gctggcgcgc gtggtcacgg gccaccgcct
     2341 gccgccgcgc acctgggcgg ccatcgtgct gggcggcctg ggcatggcct ggatgttcgc
     2401 cggtcaggcg ggcgcggcgg acgcccccgg ggcgtggctg gggtcgctcg tggcgctggg
     2461 cgtgccggcg gccgcggccg tcaactggac ggtggtgcag cgcagccagg cgcatggcga
     2521 gccgatcgat ctggtgccgt cggtcttgct gggcgcggtg atctccagcg ccatcacctt
     2581 gccgctggcc tggccgctga cggcgtcgcc gcacgacctg ggctggctgg ccttcctggg
     2641 attgacgcag ttggccatcc cctgcgtgct cgcggtgctg tgcgcccgtg tgctgccggc
     2701 ggccgaggtg gcgctgctgg gtctgctgga gatcgtgttc ggcatcctcc tggtgtggtg
     2761 gatcgtaggc gaagcgccgc gccccgaggt gctcagcggt ggtgccctgg tcatgggcgc
     2821 cctgctgggc aatgaactgc tcggctggta cgccgcacgg cgcgccgcag gggcgggcgc
     2881 cggcagcgac atcgcgcccc tcgggccggc ctgacgccag atcgtacgct ctgaccactc
     2941 aatcggccga cagcgcttgt tggtaaagcg caaacagcta cataaacgat agcataccaa
     3001 cctatcagac cggccagcgc ttcagcccaa gaacggccgt tctgggttca acctagaaac
     3061 ggcagttgga cgcccccgcc cgtgccgtga tcggcgtgcc aggcaagacg cgacgacgcc
     3121 gcgggctcct gcccgcaagg aggagcaacg ccgcatggcg cgccgagcgc ggtgcgggtg
     3181 ggtgcgtagc gtgctcaccc aggaggtgaa ggccatcgca gccgcgagtc cccgcatcga
     3241 tgcggcctgc ccgaagttga caagcggtca actgccgttt ccaggttcaa cggtggcgct
     3301 gcttgagggc gcgctcaacc tcgcgcttac cctcgcgttc cttgatggtg tcgcgcttgt
     3361 cgtgcgtggc cttgcccttg gccagggcga tttcgcactt cacgcgcccg ttcttccagt
     3421 acagcttgag cggcaccagg gtgtagccct tctgctccac cttgccgatc aggcgctgga
     3481 tctgctcggc gtgcagcagc agcttcttgg tgcgtacggc gtcgggccgc acatgggtgc
     3541 tggccgtgcc cagcgggttg atctggcagc caatcacgaa cagctcgcca gcgcggatca
     3601 ccacgtagcc gtcggtcagc tgcgccttgc ccgcgcgcag cgccttgact tcccagcctt
     3661 ccagcaccat gccggcttcg aagcgctctt cgaagaagta gttgaacgcg gctttcttgt
     3721 tttcggcgat gaccgggttg ctggtcttgg gtttcttggt ggccatcgat gcaatatggt
     3781 gggttgctcg cactttgcaa gcgcatgcgc gagtgtggcg gcgcttgagg caatgctgaa
     3841 cgcgtcccag gcgcgcgtcg cctgtcggtc agtggcggtc tgcggccctg ccgatgttcg
     3901 ccatggcctc gtccatggcg gtgccttgcg ccctgccgcc cagcccgact cagggcgcgc
     3961 ggcatcgcgg gaaccggttc agcgccgccc taaaatcggc gacaggcagc gattctatga
     4021 aatccgtcca caaatccgtc ctgatctggt acagcgcaca ggagatgttc gatctggtgg
     4081 tcgacgtcga gcactatccg gcctttctgc cctggtgcag ccatggcaag gtgatgtcgc
     4141 atgaagaggg cggaatgacc gccgaggtgg gcatcgactt caagggcgtg cgccagacct
     4201 tcgtcacccg caacacccac gtcacggggc aggaagtgca cttgcacctg gtcaaggggc
     4261 cgttttcgcg gctggaaggg cgctgggtct tcacgccggt ggggcagggc ggtcagcgcg
     4321 cctgccgcgt ggacctccga ctgaactacg ggttttccaa cgccgtactg gccaaggtgg
     4381 tcggcccggt gttcgaccgg atcgccagca gcctgatgga tgccttcatc aagcgcgccg
     4441 aacaggtcta cggcgccgcc gctgcatgag cccgccaccg cgcccggcgg cctgcgcgac
     4501 gctgctggag atcgagctga cctattcgcc cgctccgcgc cagctcgtgc agcgccggct
     4561 gcgcctgccc ggcggcagca ctgtgggcga cgcgctgaag gccgccggcc tggctagccc
     4621 ggtcacgttc gccggcggcc aggtgccgtg ggcggtgggg gtgtggggcc gaccggccgc
     4681 gcttgagcag ccgctgtgtg cgttcgatcg cgtggagttg tggcgcccct tgcgcgtcga
     4741 tcccaagctg gcgcggcgcg agcggttcgg ccagcagggc gcccgcgccg caggcctgtt
     4801 cgccaggcga cgcccgggcg ccaagccggg ctattgaccc ctcttgccct gatgggctgc
     4861 cagcacacca cggttgagtt cgctgtccac cggcaatgct actaattcag tagctgccgg
     4921 cgcttgccca caaagggccg acagcacata tcacttgcag tcgctggcga tgatggcgtt
     4981 gacccgctgg agttccgtcg cgcgctgggc gtcgtcgagc acctcgcgct cgcccttgtc
     5041 gttgacgcgc gccatgcgca tgccggattc cagcgtggcc ttggcgctgc gggcgcgttt
     5101 gcagttttct gcccgagcgg ccgccacgcg atcttcctcg gctttcttcc gggcggcctc
     5161 ggccgcctcg ttctgttttt tcttctcttc cagcgccttg tccacgccgc ccgcggcgga
     5221 cgccgcgggg cgtgcggcag cggcgcctgc tgccgcgctg gcaggctcgc tggcagaggc
     5281 agaggcagag gggccgaccg cggcggtcgc ctgcggtgcg cgcgcgcccc ttggttgcgt
     5341 cagcacgttc ttggcaggca cgtcggctgg gggcggacgg tcgctgaaca cgcggcgccc
     5401 gtccttgtcc acccactggt actgggcgtg ggcggctgcg gcggcgagga tgagtccgat
     5461 gagcagaatg cggctggttc tcataaacct gcagtgtaac cgtgcgtgac acttatggct
     5521 gtatcgcgcg cccgacagca ggcgtggggc ttatagccct gaaatgtgcc cagggtgcgc
     5581 tgtttctggc tcccatcggc cttggtaccg ctctgctcga aaatggccaa ttgtggactg
     5641 gcggcgcttc tgctggcggc gcttctgcgg ggcttcggat aaaatatgag ctttagcggg
     5701 ttgcggcgca gcccccacgg tgcagattca caaggacgag aaaggcagca tcggttatga
     5761 caccgcgaac taccaccgag ggtttcacca ccgtcgtctg acggtgcggg gttcgcgcct
     5821 gttcctttcg tcctttccca gatcaagcgc ggcccggtcc gcgctttttt gtttttccac
     5881 gcaaggtttc cccccatgat tcacatcacg cttcccgacg gctcccagcg cgagttcccc
     5941 ggcccggtga cggtggccga ggtggcgcag tccattggcg ccggtctggc caaggcggcc
     6001 ctgggcggca aggtggacgg caaactggtc gacaccagct tccgcatcga tcacgacgtg
     6061 cagctggcca tcatcaccgc caaggacgcg caagggctgg agatgatccg ccactccacg
     6121 gcccacctgc tggcctatgc ggtcaaggag ctgtttccgg aggcgcaggt caccatcggc
     6181 ccggtgatcg agaacggttt ctactacgac ttcagctaca agcgaccctt cacgcccgag
     6241 gacctggcgg cgatcgagaa gaagatggaa cagctggccg ccaaggacga gccggtgctg
     6301 cgccgcgtgc tgccgcgcga cgaggcggtc gaatacttca aaggtctggg cgagcattac
     6361 aaggccgaga tcatcgccag catccccgcc aatgaagacg tcagtctgta ccgtgaaggc
     6421 gccttcgagg acctgtgccg cggcccccac gtgcccagca cgggcaagct gcggcatttc
     6481 aagctgatga aggtggcggg cgcctactgg cgcggcgacc accgcaacga gatgctgcag
     6541 cgcatctacg gcacggcctg ggccagcaag gatgatctca aggcctacct gaccatgctg
     6601 gaggaggccg aaaagcgcga ccaccgccgt ctggggcgcg agctcgactt gtttcacatc
     6661 gacgagcacg cccccggcac ggtgttctgg caccccaagg gctggaccgt gtggcaggag
     6721 gtcgagcagt acatgcgccg cgtctaccgc gacaacggct accacgaagt caagggcccg
     6781 cagatcctgg acaagacgct gtgggagaag accggccact gggacaagta ccgcgagaac
     6841 atgttcgtca cggaatcgga aaagcgcgac tacgcgctca agccgatgaa ttgccccggc
     6901 cacatcctga tctacaagca gggcgtcaag agctaccgcg acctgccact gcgctacggg
     6961 gaattcggca actgccaccg caacgaaccg tcgggtggcc tgcacggcat catgcgggtg
     7021 cgcggcttca cgcaggatga cgggcacatc ttctgcaccg aagacatgat ccaggccgaa
     7081 gtcgcggcct tcacgcggtt gctgcagaag gtctacaggg acttcggttt caccgagatc
     7141 atctacaagc tgtccacgcg cccggccgcg cgcatcggca ccgaagagag ctgggacaag
     7201 gccgaggccg cgctggccga gggtctgcgc gcgtcgggct gcgagttcag ttacctgccg
     7261 ggcgagggcg cgttctatgg ccccaagatc gagtacacgc tgaaagacgc gctgggccgc
     7321 gaatggcagt gcggcacgat ccaggtggac ccgaacatgc ccgagcggct ggacgccgaa
     7381 tacgtgggcg aggacggcgc gcgtcaccgc cccatcatgc tgcaccgtgc catcgtcggc
     7441 agcctggagc gcttcatcgg catcctgatc gagcagcacg ccggtgcgct gccggtgtgg
     7501 ctggcgccgg tgcaggtgat ggtgaccggc atcaccgacg cgcaggccga ctacgcccgg
     7561 agcgtggcga aaacgctgcg aaatcaaggg tttagggccg aaaccgacct gcgcaacgag
     7621 aagatcacgt ataaaatacg cgagcattcg ttgcaaaagc ttccctacat cctggtcgtg
     7681 ggcgacaagg agcgggaagc cggtgccgtt gcggtgcgtg cccgaggcaa tgtggacctc
     7741 ggcgtgatgt ctctcgaagc ctttgccaag cgcatcggcg aagacatcgc ttccaaagcc
     7801 tgatttctga ccgaaagcag ctctggccag catgcaaggc gcggcgttcg ctatttgttc
     7861 gatagcatac tgaactgagg tttcaaccat cgctaccaat tttcgtgacc gccgccaacg
     7921 tgaagagcgc gcgcatcgtc tgaaccgaga aatcatggcg cccgaagtgc gcctgaatgg
     7981 ccccaacaac gaacccctgg ggatcgtgcc actgcaggaa gccctgcgca tggcgggcga
     8041 tctggatgtg gatctggtcg aagtggtggc cacggccaac ccgccggtct gccgcctgat
     8101 ggactacggc aagttcaagt accaggagca gaagaaggcc gccgaggcca aggccaagca
     8161 gacggtcatc gagatcaagg aagtcaagtt ccgccccggt accgacgatg gcgattacca
     8221 gatcaagatg cgcaacattc gccgctttct ggccgatggc gacaaggtca aggttacgct
     8281 gcgctttcgc ggccgcgaaa tcacccacca ggaactgggg ctggcgctgc tcgatcgcat
     8341 ccgtgccgat ctggccgaca gcgtggtggt ggagcagttt cccaagctcg aagggcgcca
     8401 gatgatcatg atgctggcac ctgcgcgcaa gaagacgggt ggcggagcgg ccaccaaggc
     8461 ggcgggcgcg gaccagcagc cagcagccgc ctgaagggcg acgcagacga tcgaacttga
     8521 atttgccggg ttcgcccggt gaaccggcag gctgcaaggc ctgccagcag aagtggctcg
     8581 gggccaacaa gggtgcaaag tcgttttgca cacgcctcgc gagcacaaag ccaacaggag
     8641 catgtcacat gcccaaaatg aagaccaaga gcagcgcgaa gaagcgtttt cgcgtccgtc
     8701 ccggtggcac cgtcaagcgc
//
LOCUS       DAOOOG010000109         8639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_9563_length_8639_cov_24.449208, whole genome shotgun sequence.
ACCESSION   DAOOOG010000109 DAOOOG010000000
VERSION     DAOOOG010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8639
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9563_length_8639_cov_24.449208"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1050
                     /locus_tag="PK242_08845"
     CDS             103..1050
                     /locus_tag="PK242_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ94166.1"
                     /translation="MPAARYQQAPTAIEMIASGAPVSALQLHTPRGGRVAAQLYQPPA
                     GRPVRWQVVIGPAMGVPQRFYAGFATWLAAQGARVLSFDYRGQADSLALLPHARLRQI
                     DATLHDWRADYEAATLHLHALAPDVPLLLVGHSLGAQLPGLFERSGHIDGLLAVAAGS
                     GYWRQNAPRLRSRALLMWYGMVPLLTPLFGYFPGRRLGAVGDLPAGVIRQWRRWCLHP
                     HYSGAEGPEARQRYAAVRFPIHALSVTDDEMMTLPGTQSLLALYAGAPHGIERVAPAD
                     HGLPRIGHLGWFHPRFEATLWPLTLRALGELAARTGPAT"
     gene            1117..1986
                     /locus_tag="PK242_08850"
     CDS             1117..1986
                     /locus_tag="PK242_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOZ94167.1"
                     /translation="MTRSALMTRHPFDDAVALAPLAASADERGPQAPGHFQGAPHAAY
                     GNMVGPFGGITAAQALAAVLRHPARLGEPVALTVNYAAALIEAPFRIEARPVRTNRST
                     QHWTVALMQTDAQGVDGVMLTATCVTAVRRATWSAVEVPRPAAPAPQQVPRAVPPKRV
                     RWIERYDMRPLAGPMPLQWDGSEADSLTRLWVRDDPPRPLDFLSLTALADVFFPRVWR
                     RRARMTPAGTVSMSVYFHAGADELAATGEGYLLGQARAQSFFNGFFDQSAELWNEAGH
                     LLATTHQVVYFKE"
     gene            1983..2714
                     /locus_tag="PK242_08855"
     CDS             1983..2714
                     /locus_tag="PK242_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ94168.1"
                     /translation="MSMTRPVALVVGAGDATGGAIARRFAAGGYTACVTRRDAARLDE
                     LRHAIEAAGGICHAFGSDARKEDEVVALVDTIERDIGPIEVLVFNIGANVPCSVLEET
                     ARKYFKIWEMACFGGFLNGREVARRMVGRGRGTIIFTGATASVRGAAGFAAFAGAKHA
                     LRALAQSMARELGPQGIHVAHCIIDGAIDTAFIRERFPERYALRAQDGILRPDSIAEA
                     YWMLHRQPRDAWTHELDLRPYMEKF"
     gene            2711..3301
                     /locus_tag="PK242_08860"
     CDS             2711..3301
                     /locus_tag="PK242_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HOZ94169.1"
                     /translation="MMKSFEFWFDFGSPAAYLAWTQIPAIEASTGAQALWRPMLLGGV
                     FQATGNQSPAAVAAKGRYIFTDFDRFARRYGVPLRRNPHFPIHTLLPMRGAVGLQRAQ
                     PERLADYCRAMFQAIWVDARNLNEPAEVGAVLAAAGFDPQALLALANEAQTKQALKDA
                     TEEAVRRGVFGAPTFFVGEQMFWGQDRIDFLKEALQ"
     gene            3298..4062
                     /locus_tag="PK242_08865"
     CDS             3298..4062
                     /locus_tag="PK242_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOZ94170.1"
                     /translation="MNEILSQSEGGVLTLTFNRVERKNSINVAMYDALATAIERAVDD
                     AATRVLLIQGHETVFSAGNDIEDFLRNPPAGKDSPVFRFLRGIATFPKPLLAAVCGPA
                     VGVGTTLLFHCDLVYAGDNAAFSVPFVNLGLCPEAASSLLLPQMFGYHRAAEALLLGE
                     PFMAEAALEVGLVNRVLPPTECNAFARQQARKLAAKPLSSLITTKQLMKGAQQEAVLR
                     RMDEEGAHFRRMLGEPAAREAMTAFMEKRKPDFSAC"
     gene            4056..4310
                     /locus_tag="PK242_08870"
     CDS             4056..4310
                     /locus_tag="PK242_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94171.1"
                     /translation="MLKSAASSDAVPRSGAQQLSSKAQAGFQRMLKSAASSDAVPRSG
                     AQQLSSKAQAGFQRMLKSAASTAAGSPRGRGNVRRRFSHP"
     gene            4318..4806
                     /locus_tag="PK242_08875"
     CDS             4318..4806
                     /locus_tag="PK242_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOZ94172.1"
                     /translation="MTRPPMEPPPIAFEPEFADGLRLMFEQQVVFNRVIGLKLLSIRP
                     DGVQGRIDMREELIGHFGHRRIHGGVISAGLDAMGGIATMAAIGARHMDEPPATRLQR
                     FGKLGTIDLRIDYLRPGIGTHFLLSAEVLRLGSRVASTRMAFHGADGKLLASGAAAYM
                     VS"
     gene            complement(4866..5225)
                     /locus_tag="PK242_08880"
     CDS             complement(4866..5225)
                     /locus_tag="PK242_08880"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOZ94173.1"
                     /translation="MKVIPMPADANANGDIFGGWVMAQVDLAGAVLPARYAQGRMATV
                     AVNQFVFKQPVRVGDILSFYARMERIGRSSLTVNVGVYAERFDTQGRYIKVTEATLTY
                     VAIDDSGRPRPVPRPEM"
     gene            5375..7234
                     /locus_tag="PK242_08885"
     CDS             5375..7234
                     /locus_tag="PK242_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HOZ94174.1"
                     /translation="MRGGGERSLSLAPGETASRQRSDAATLRRLLPYLWQYKGRVLAA
                     IAFMVGAKLANVGVPLLLKRLVDAMTLPAGGSAALLVVPVALLLAYGLLRLLNSVFNE
                     LRELVFAKATHGAARAIALKTFEHLHGLSLRFHLERQTGGMTRDIERGVRGIESLVSF
                     ALFNLAATLVEVLLVLFILARQFDLWFAVITLTALVLYIAFTVAITQWRIQFRRQANQ
                     FDSAAHSKAIDSLLNYETVKYFNNEQFEARRYDESLEQLRRAQLKARSTLGLLNSGQQ
                     LIIAVGLVAMLWRATEGVAAGRMTIGDLVMVNAFMIQIYIPLNFLGVIYREIKQNLTD
                     LDKMFTLMDKEQEVADAPGAQALHTNGPVDLRFENVHFAYDPARPILRGVSFDVPAGH
                     KVAVVGPSGAGKSTLARLLFRFYDVGPEGGRILIAGQDIRRVTQASVRRAIGIVPQDT
                     VLFNDTVAYNIRYGRPDASDAEVESAARAAHIHDFIMGTPAGYATMVGERGLKLSGGE
                     KQRVAIARTLLKDPPILVFDEATSALDSHNERAIQAELKSAARGKTTLVIAHRLSTVV
                     DAHEILVMEQGQIVERGTHLQLLAARGRYARMWALQQSAEPAAAPSEPESVTD"
     gene            7369..8265
                     /locus_tag="PK242_08890"
     CDS             7369..8265
                     /locus_tag="PK242_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOZ94175.1"
                     /translation="MKKQLLTLAVLALAAGTASAQATDTLAKIKDRGAVNLGVRDSSG
                     LAFTLGGGKYVGFHTEMAERIIDDLGKSLGKPLKINYQVITSQNRIPLIQNGTIDFEC
                     GSTTNNKARQKDVDFAFTTYVEEVRIAVKANSGIKSIKDLNGRTVATTTGTTSVQTLR
                     KNERAGGIDFKEVMGKDHADSFLLLESGRADAFVMDGSILAANIAKSKSPKDFAIVGE
                     VLSVEPIACMLPKGDAKLKKAIDDSIVRQIKDGSLTKLYDKWFMQPIPPNNVTLNMPA
                     SESTKAAWASPNDKPMEDYAAK"
     gene            8378..>8639
                     /locus_tag="PK242_08895"
     CDS             8378..>8639
                     /locus_tag="PK242_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOZ94176.1"
                     /translation="MAMDWSVFCKDTLTGEVMPGCFGSGQDITYLNWLFTAWGWTVAV
                     SLTALVVAMVMGSIVGVIRTLPDRPGLVRLGNAWVELFRNIPL"
BASE COUNT         1351 a         2977 c         2954 g         1357 t
ORIGIN      
        1 ctcaagtacg gcatggtcac catgtgcgtg ggcatgggcc agggcgcggc cggcatcttt
       61 gagcgggtgt gacgccggtg ggccgttggt tccagatgat tcatgcctgc ggcgcgctac
      121 cagcaagcgc cgacagctat tgaaatgata gcttccggag cgccggtgtc ggccctgcaa
      181 ctgcacacgc cgcggggcgg tcgggtggcg gcgcagctgt accagccacc cgccggccgt
      241 cctgtgcgct ggcaggtggt gatcgggccg gcgatgggcg tgccgcaacg cttctacgcc
      301 ggtttcgcca cctggctggc ggcgcagggt gcgcgcgtgc tcagcttcga ctaccgcggg
      361 caggccgatt cgctggcgct gctgccgcac gcgcggctgc gccagatcga cgccacgctg
      421 cacgactggc gcgccgacta cgaggccgcg acgctgcacc tgcatgcgct ggcgcccgac
      481 gtgccgctgc tgctggtcgg gcacagcctg ggcgcgcagc tgcccggcct gttcgagcgc
      541 agcgggcaca tcgacggcct gctggcggtg gcggccggca gcggctactg gcgccagaac
      601 gcgccgcgcc tgcgcagccg ggcgctgctc atgtggtacg gcatggtgcc gctgctgacg
      661 ccgctgttcg gctactttcc gggccggcgc ctgggggcgg tgggcgatct gccggccggt
      721 gtgatccgcc agtggcggcg ctggtgcctg cacccgcact acagcggcgc cgaagggccc
      781 gaggcgcgcc agcgctacgc ggcggtgcgc tttcccatcc atgcgctgtc ggtcaccgac
      841 gacgagatga tgaccctgcc cggcacgcaa agcctgctgg cgctgtacgc cggtgcgccg
      901 cacggcatcg agcgcgtggc gccggccgac cacggcctgc cgcgcatcgg ccatctgggc
      961 tggtttcacc cgcgcttcga agccacgctg tggccgctca ccctgcgggc gctgggcgaa
     1021 ctggcggccc gcaccggccc tgccacctga gcgacagcgc gcgcgccgct gcgtgctaag
     1081 gtcacgcgtt ccctgtctgt ccacctgatc ctgcacatga cacgttccgc cctcatgacc
     1141 cgtcacccgt tcgacgacgc ggtggcgctg gcgccgctgg ccgccagcgc cgacgagcgc
     1201 ggcccacagg cgccgggcca tttccagggg gcgccgcacg ccgcctacgg caacatggtg
     1261 gggccgttcg gtggcatcac ggcggcgcag gcgctggcgg cggtgctgcg ccatccggcg
     1321 cggctgggcg agccggtggc gctgaccgtc aactacgcgg cggcgctgat cgaggcgccg
     1381 tttcgcatcg aagcgcgtcc ggtgcgcacc aaccgctcca cccagcactg gacggtggcg
     1441 ctgatgcaga ccgacgccca gggcgtcgat ggcgtgatgc tcaccgccac ctgcgtcacc
     1501 gccgtgcggc gtgccacctg gagcgccgtc gaggtgccgc ggccggcggc gccggcgccg
     1561 cagcaggtgc cgcgcgccgt gccgcccaag cgcgtgcgct ggatcgaacg ctacgacatg
     1621 cggccgctgg ccggccccat gccgctgcag tgggacggca gcgaggccga cagcctgacg
     1681 cgcctgtggg tgcgcgacga cccgccgcgc ccgctggact ttctgagcct gacggcgctg
     1741 gccgacgtgt tcttcccgcg cgtgtggcgt cgccgcgcgc gcatgacgcc ggccggcacg
     1801 gtgtcgatgt cggtctattt tcacgccggg gccgacgaac tggccgccac cggcgagggc
     1861 tacctgctcg gtcaggcgcg ggcgcagtcc ttcttcaacg gtttcttcga ccagagcgcc
     1921 gagctgtgga acgaagccgg tcatctgctg gccaccacgc accaggtggt gtacttcaag
     1981 gaatgagcat gacgcgcccc gtggccctgg tggtgggcgc cggcgacgcc accggcggcg
     2041 ccatcgcgcg gcgctttgcc gccggcggct ataccgcctg cgtgacgcgg cgcgacgccg
     2101 cccggctgga cgagctgcgc cacgccatcg aagcggcggg cggcatctgt cacgccttcg
     2161 gctccgacgc acgcaaggaa gacgaggtgg tggcgctggt cgacaccatc gagcgcgaca
     2221 tcggcccgat cgaggtgctg gtgttcaaca tcggcgccaa cgtgccctgc agcgtgctgg
     2281 aagaaaccgc gcgcaagtac ttcaagatct gggaaatggc ctgcttcggc ggctttctga
     2341 acgggcgcga agtggcccgg cgcatggtgg ggcgcgggcg cggcaccatc atcttcaccg
     2401 gcgccacggc gtcggtgcgc ggggcagccg gctttgccgc ctttgccggc gccaagcatg
     2461 cgctgcgtgc gctggcccag agcatggcgc gcgagctcgg gccgcagggc atccatgtgg
     2521 cgcactgcat catcgacggc gccatcgaca ccgccttcat ccgcgagcgc ttccccgaac
     2581 gctatgcgct gcgcgcgcag gacggcatcc tgcggcccga cagcatcgcc gaggcctact
     2641 ggatgctgca ccggcagccg cgcgacgcct ggacgcacga gctggatctg cgtccctaca
     2701 tggagaagtt ctgatgaagt ccttcgaatt ctggttcgat ttcggcagtc cggcggccta
     2761 cctggcgtgg acgcagatcc cggccatcga ggccagcacc ggcgcgcagg cgctttggcg
     2821 gccgatgctg ctgggcggcg tgttccaggc caccggcaac cagtcgcccg ccgccgtggc
     2881 ggccaagggg cgctacatct tcaccgactt cgaccgcttt gcccgccgct acggcgtgcc
     2941 gctgcgccgc aacccgcatt tcccgatcca caccctgctg ccgatgcgcg gcgccgtggg
     3001 cctgcagcgc gcccagcccg agcgtctggc cgactactgc cgcgccatgt tccaggccat
     3061 ctgggtggac gcgcgcaacc tgaacgagcc ggccgaggtc ggcgcggtgc tggcggcggc
     3121 gggcttcgat ccgcaggcgc tgctggcgct ggccaacgaa gcgcagacca agcaggccct
     3181 gaaggacgcc accgaggaag cggtgcgccg cggtgtgttc ggcgcgccga ccttcttcgt
     3241 cggcgagcag atgttctggg gccaggaccg catcgatttt ctcaaggaag cactgcaatg
     3301 aacgagatcc tgtcgcaatc cgaaggcggc gtgctgacgc ttaccttcaa ccgcgtcgag
     3361 cgcaagaatt ccatcaacgt cgccatgtac gacgcgctgg ccacggccat cgagcgcgcg
     3421 gtggacgacg ccgccacgcg ggtgttgctg atccagggcc acgagacggt gttttcggcc
     3481 ggcaacgaca tcgaggactt tctgcgcaac ccgccggccg gcaaggattc gccggtgttc
     3541 cgcttcctgc gtggcatcgc cacctttccc aagccgctgc tggcggccgt gtgcggcccg
     3601 gcggtgggtg tgggcaccac gctgctgttt cactgcgacc tggtctatgc gggcgacaac
     3661 gcggcgtttt cggtgccctt cgtcaacctg ggcctgtgcc ccgaagccgc atccagcctg
     3721 ctgctgccgc agatgttcgg ttaccaccgc gcggccgaag cgcttctgct gggcgagccc
     3781 ttcatggccg aagcggcgct ggaggtcggc ctggtcaacc gcgtgctgcc gcccaccgag
     3841 tgcaacgcct ttgcccggca gcaggcgcgc aagctggccg ccaagccact gtccagcctg
     3901 atcaccacca agcagctgat gaagggcgca cagcaggagg cggtgctgcg gcgcatggac
     3961 gaagagggcg cgcatttccg tcgcatgctg ggcgagcccg ccgcccgcga agccatgacc
     4021 gccttcatgg aaaagcgcaa gccggatttc agcgcatgct gaaatccgcc gcatcgtcag
     4081 atgcggtccc gcgcagcggg gcgcagcagc tttcgtcaaa agcgcaagcc ggatttcagc
     4141 gcatgctgaa atccgccgca tcgtcagatg cggtcccgcg cagcggggcg cagcagcttt
     4201 cgtcaaaagc gcaagccgga tttcagcgca tgctgaaatc cgccgcatcg accgctgccg
     4261 ggtcgccccg cgggcgtggt aacgtcaggc gcaggttcag tcacccgtga gttccgcatg
     4321 acccgtcccc cgatggagcc accgcccatc gccttcgagc ccgagttcgc cgatgggctg
     4381 cgcctgatgt tcgagcagca ggtggtgttc aaccgcgtga tcggcctgaa gctgctcagc
     4441 atcaggcccg acggcgtgca ggggcgcatc gacatgcgcg aggagctgat cggtcacttt
     4501 ggccaccggc gcattcacgg cggcgtgatc agcgccggcc tggacgccat gggcggcatc
     4561 gccaccatgg cggcgatcgg cgcgcgccac atggatgaac cgcccgccac ccgcctgcag
     4621 cgctttggca agctgggcac catcgacctg cgcatcgact acctgcggcc aggcataggc
     4681 acgcacttcc tgctgtcggc cgaggtgctg cgcctgggct cgcgcgtggc cagcacccgc
     4741 atggccttcc atggggccga cggcaagctg ctggccagcg gggcggcggc ctacatggtc
     4801 tcctgaaagc tataaaaaca atagctttcg gcgcttgtcc tgagcgcgct ggaggcctgt
     4861 ttcattcaca tttcggggcg tggcaccggg cgcggccgcc cgctgtcgtc gatcgccacg
     4921 taggtcaggg tggcctcggt caccttgatg tagcggccct gggtgtcgaa gcgctcggcg
     4981 tacacgccca cgttgacggt gagcgagctg cggccgatgc gctccatgcg ggcgtagaac
     5041 gacaggatgt cgcccacccg caccggttgc ttgaagacga actggttcac cgccaccgtg
     5101 gccatgcggc cctgggcgta gcgcgccggc agcacggcgc cggccaggtc gacctgggcc
     5161 atcacccagc cgccgaagat gtcgccgttg gcattggcgt cggccggcat ggggatcacc
     5221 ttcatcacca gctcgtgatc ggccggcggg gtttcggccg gcgcctcgac cgggtgctcg
     5281 ttggggcggc gcaaatggcc ggtgaccgtg ctggacaggt gggacatggg cagaatcgac
     5341 ggctcggatg ttggcaatga cgggattctg aaccatgcgc ggtggcggcg aacgctccct
     5401 ttcccttgct cctggcgaga cggcctcgcg ccagcgctcg gacgcggcca cgctgcgccg
     5461 cctgctgccc tatttgtggc agtacaaggg ccgcgtgctc gcggccatcg ccttcatggt
     5521 cggcgccaag ctggcgaacg tgggcgtgcc tctgctgctc aagcgcctgg tcgatgccat
     5581 gacgctgccc gcgggcggca gcgccgcgct gctggtcgtg cccgtggccc tgctgctggc
     5641 ctatggcctg ctgcgcctgc tgaactcggt gttcaacgag ctgcgcgagc tggtgtttgc
     5701 caaggccacg catggcgcgg cgcgcgccat tgcgctcaag accttcgagc acctgcacgg
     5761 cctgtcgctg cgctttcacc tggagcgcca gaccggcggc atgacgcgcg acatcgagcg
     5821 cggcgtgcgc ggcatcgagt cgctggtgtc gttcgcgctg ttcaaccttg cggccacgct
     5881 ggtcgaggtg ctgctggtgc tgttcatcct cgcgcgccag ttcgacctct ggtttgccgt
     5941 catcacgctc acggcgctgg tgctctacat cgcgttcacc gtggccatca cgcagtggcg
     6001 catacagttt cggcgccagg cgaaccagtt cgactcggcg gcgcacagca aggccatcga
     6061 ctcgctcttg aactacgaga ccgtcaaata cttcaacaac gagcagttcg aggcgcggcg
     6121 ctacgacgaa agcctggagc agctgcgccg cgcgcaactc aaggcgcgca gcacgctggg
     6181 cctgctcaac agcggccagc agctcatcat cgccgtgggg ctggtggcca tgctctggcg
     6241 cgccaccgag ggcgtggccg ccgggcgcat gacgattggc gacctggtca tggtcaacgc
     6301 cttcatgatc cagatctaca tcccgctcaa cttcctgggc gtgatctacc gcgagatcaa
     6361 gcagaacctg accgacctgg acaagatgtt cacgctgatg gacaaggagc aggaggtggc
     6421 cgacgcgccc ggcgcccagg cgctgcacac caacggcccg gtcgatctgc gtttcgagaa
     6481 cgtgcacttc gcctacgacc cggcgcgccc gatcctgcgc ggcgtcagct ttgacgtgcc
     6541 ggccgggcac aaggtggcgg tggtggggcc gtcgggggcg ggcaagtcca cgctggcacg
     6601 gctgctgttt cgcttctacg acgtcgggcc cgaggggggc cgcatcctga ttgccggcca
     6661 ggacatccgc cgcgtcaccc aggccagcgt gcgccgggcc atcggcatcg tgccgcagga
     6721 cacggtgctg ttcaacgaca ccgtggccta caacatccgt tacggccggc ccgacgccag
     6781 tgacgccgag gtggagagtg ccgcgcgagc cgcccacatc cacgacttca tcatgggcac
     6841 gccggccggc tacgccacca tggtcggcga gcgcgggctg aagctgtcgg gcggcgaaaa
     6901 gcagcgcgtg gcgatcgccc gcaccttgct caaggacccg ccgatcctgg tcttcgacga
     6961 ggccaccagc gccctcgatt cgcacaacga gcgcgccatc caggccgaac tgaagagcgc
     7021 cgcgcgcggc aagaccacgc tggtgatcgc gcaccgcctg tccaccgtgg tggacgcgca
     7081 cgaaatcctg gtgatggaac agggccagat cgtcgagcgc ggcacgcacc tgcaactgct
     7141 ggccgcgcgt ggccgctacg cccgcatgtg ggcgctgcag caaagcgccg agccggcggc
     7201 cgcaccatcc gagcccgaaa gcgtgaccga ttgagcagaa accgggccgg ccgttcagcc
     7261 gatgcacaca cccagggtta gtccgtagcc gtgggtacgt agaatgcgtt gagagggtcc
     7321 cgccttccgt gcgaaggatc ccttgttcat ctgctgaaag gaaccggaat gaaaaaacag
     7381 cttctgaccc tggcggtgct ggccctggcg gccggcacgg cgtctgcaca ggccaccgac
     7441 acgctggcca agatcaagga ccgcggcgcc gtcaacctgg gtgtgcgcga ctcctccggc
     7501 ctggccttca cgctgggggg cggcaaatac gtcggcttcc ataccgaaat ggccgagcgc
     7561 atcatcgacg acctgggcaa gagcctgggc aagccgctga agatcaacta ccaggtgatc
     7621 acctcgcaga accgcatccc gctgatccag aacggcacca tcgacttcga gtgcggctcc
     7681 accaccaaca acaaggcgcg ccagaaggac gtcgacttcg ccttcaccac ctacgtggaa
     7741 gaagtgcgca tcgccgtcaa ggccaattcc ggcatcaagt cgatcaagga cctgaacggc
     7801 aggacggtgg ccaccaccac cggcaccacc agcgtgcaga cgctgcgcaa gaacgagcgc
     7861 gccggcggca tcgacttcaa ggaggtgatg ggcaaggacc acgccgacag cttcctgctg
     7921 ctcgagtccg gccgcgccga cgccttcgtg atggacggct ccatcctggc ggccaacatc
     7981 gccaagtcga aaagccccaa ggacttcgcc atcgtgggcg aagtgttgtc ggtggagccg
     8041 atcgcctgca tgctgcccaa gggcgacgcc aagctgaaga aggcgatcga cgacagcatc
     8101 gtgcgtcaga tcaaggatgg ctcgctgacc aagctgtacg acaaatggtt catgcagccg
     8161 atcccgccca acaacgtgac cctcaacatg ccggcgtcgg aaagcaccaa ggccgcctgg
     8221 gccagcccga acgacaagcc gatggaggac tacgcggcca aatgagccac gggcgacacg
     8281 cctgaggcca tgtccacgac gcccacttgt ccgcaggtgg gcgtttgtgg ttatatgagg
     8341 tgctgatcgc ggcgcggtcg gtccaaggag ggttgcaatg gcgatggatt ggtcggtgtt
     8401 ctgcaaggac accctgaccg gcgaggtcat gccgggctgt ttcggcagcg ggcaggacat
     8461 cacctacctc aactggctgt tcacggcctg gggctggacg gtggcggtgt cgctgaccgc
     8521 cctggtggtg gccatggtga tgggctccat cgtgggcgtg atccgcaccc tgccggatcg
     8581 gcccgggctg gtgcgcctgg gcaacgcctg ggtcgagctg tttcgcaaca tcccgctgc
//
LOCUS       DAOOOG010000110         8604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_9616_length_8604_cov_24.393730, whole genome shotgun sequence.
ACCESSION   DAOOOG010000110 DAOOOG010000000
VERSION     DAOOOG010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9616_length_8604_cov_24.393730"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PK242_08900"
     CDS             <1..340
                     /locus_tag="PK242_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOZ94177.1"
                     /translation="AMGETERRRAKQIAFNAEQGITPRGIVKQVRDLIDGVYGERGER
                     QVLAQGRPAVQEIALSEKDLAREIKRLEKQMLEHARNLEFEQAARVRDQLHALKQGAF
                     GAPGTDSPSG"
     gene            503..1036
                     /gene="iscR"
                     /locus_tag="PK242_08905"
     CDS             503..1036
                     /gene="iscR"
                     /locus_tag="PK242_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HOZ94178.1"
                     /translation="MRLTTKGRFAVTAMIDLALRQDSGPVTLAAISQRQRISLSYLEQ
                     LFGKLRRHELVESTRGPGGGYSLGRKADDITVADIIVSVDEPLDATHCAGKQNCQGGD
                     GPCMTHDLWTALNDRMLDFLQSVTLQKLVDDQHAKGIEIEGKPATKRAISSAPVVKPI
                     RVNAPNSVFALGGLTNK"
     gene            1122..2342
                     /locus_tag="PK242_08910"
     CDS             1122..2342
                     /locus_tag="PK242_08910"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HOZ94179.1"
                     /translation="MSQTPHFPIYMDYGATTPVDPRVVDAMVPWLREHFGNPASRSHA
                     WGWEAEQAVETAREQVAALIGADPREIVWTSGATESDNLAIKGAAQFYKGKGRHLITV
                     RTEHKAVLDVMRELERQGFEVTYLEVQPDGLLDLEALRAAIRPDTILASVMLVNNEIG
                     VIQDVQAIGRLCREQGVLLHVDAAQATGKVAIDLATLPVDLMSLASHKTYGPKGIGAL
                     YVRRKPRVRLEAQMHGGGHERGMRSGTLPTHQIVGMGEAFRIAREEMARDSEKARALH
                     RRLVDGLKDIEEAYLNGHPTQRVPHNVNMSFNYVEGESLMMGIKGLAVSSGSACTSAS
                     LEPSYVLRALGRSDELAHSSLRITIGRFTTEQEIDYAIRAIRDNVARLRELSPLWEMH
                     QDGIDLSTISWAAH"
     gene            2412..2795
                     /gene="iscU"
                     /locus_tag="PK242_08915"
     CDS             2412..2795
                     /gene="iscU"
                     /locus_tag="PK242_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HOZ94180.1"
                     /translation="MAYSQQVIDHYENPRNVGSFDKGDASVGTGMVGAPACGDVMRLQ
                     IKVNPDTGVIEDARFKTYGCGSAIASSSLVTEWVKGKTLAQATELKNSQIADELALPP
                     VKIHCSILAEDAIKAAVEDYRKKHA"
     gene            2871..3194
                     /gene="iscA"
                     /locus_tag="PK242_08920"
     CDS             2871..3194
                     /gene="iscA"
                     /locus_tag="PK242_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HOZ94181.1"
                     /translation="MTVTLTEAAARHVSKYLAQRGKGVGVRLGVKTTGCSGLAYQLEY
                     VDEVAPEDALFEQHGVKLMVDPKSLAYIDGTELDYVREGLNEGFRFNNPNERDRCGCG
                     ESFRI"
     gene            3244..3759
                     /gene="hscB"
                     /locus_tag="PK242_08925"
     CDS             3244..3759
                     /gene="hscB"
                     /locus_tag="PK242_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HOZ94182.1"
                     /translation="MQLHSTDFELFDLPPRFAQDGAVLEARWKALQREVHPDRFAAQG
                     AAAQRVAAQWSARINEAHRRLKDPQRRAAYLCELHGVPVDAERHTAMPPEFLMQQMAL
                     REQLDEAGGAPALDAIAAQVAGLRHELLQALAQQQDVERDWSAAARSVRALMFLDKLA
                     ADVDRRRDTLA"
     gene            3798..5657
                     /gene="hscA"
                     /locus_tag="PK242_08930"
     CDS             3798..5657
                     /gene="hscA"
                     /locus_tag="PK242_08930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HOZ94183.1"
                     /translation="MALLQISEPGQSPDPHQRRIAVGIDLGTTHSLVAAVRHGVAECL
                     PDAQGRVLLPSVVRYPAEGGRQIGHAAASDVPAEAAHTIGSFKRLMGRGLGDIAGHER
                     LPYPLLDRPGMVAVGTPQGEKSPVELSAEILAILRQRAEDSLGGQLHGAVITVPAYFD
                     DAQRQATKDAARLAGIPVLRLINEPTAAAVAYGLDRAAEGVYAVFDLGGGTFDLSIMR
                     LTQGVFEVVATGGDTALGGDDYDHALADWVLARCAVQARTAADKAALARAARQAKEAL
                     SATETVAFRAHLTSGELVFDLKADDFAQATQALTRRCLDTARRVLRDARLTPDDIQGV
                     VLVGGSTRMPVIRQAVAGLFGRAPLTDLNPDEVVALGAAIQAQQLAGHRPPGDLLLLD
                     VIPLSLGLETMGGLVERIVPRNQTIPTAMAQDFTTYRDGQTALALHVVQGERDLVQDC
                     RSLARFELRGIPPMAAGAARIRVTFSVDADGLLSVSAREQTSGAQAQIEVRPSYGLSD
                     RQIAQMLQDSFGTAEADVRARALAEARVEAERLRLATQGALDADGALLSAPERRAIEA
                     LMQTLRERAADGSDAAAIAAATEALAQGTEAFAARRMNAGIRRALTGRNVHSL"
     gene            5661..5999
                     /gene="fdx"
                     /locus_tag="PK242_08935"
     CDS             5661..5999
                     /gene="fdx"
                     /locus_tag="PK242_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HOZ94184.1"
                     /translation="MPTIQVLPHPEHCPDGARIQAQVGDSICEALLAHGIEIEHACDM
                     ACACTTCHVIVRQGYDSLASPEEEEEDLLDRAWGLEPQSRLSCQAVVARADLTVEIPR
                     YSINHAREKR"
     gene            6037..6750
                     /gene="dnaQ"
                     /locus_tag="PK242_08940"
     CDS             6037..6750
                     /gene="dnaQ"
                     /locus_tag="PK242_08940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HOZ94185.1"
                     /translation="MTRQIVLDTETTGLSAESGDRIIEIGCVELVGRKLTGNHLHFYL
                     NPERDSHEDALKVHGISNEFLRDKPRFAEVADEILRYVEGTELIIHNAAFDLGFLDKE
                     LERLGRPPLRSFVAGVTDTLAMAKQLYPGKRNSLDALCERLGVDNSGRTLHGALLDAE
                     LLADVYISLTRGQEALLIDLGEVGAAGGDAPRIDLTGLELPVLTANAQELAAHEDVLA
                     QIDKASGGKTVWRRVETGL"
     gene            6776..6850
                     /locus_tag="PK242_08945"
     tRNA            6776..6850
                     /locus_tag="PK242_08945"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6808..6810,aa:Val,seq:cac)
     gene            6944..7543
                     /locus_tag="PK242_08950"
     CDS             6944..7543
                     /locus_tag="PK242_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOZ94186.1"
                     /translation="MLLDADDCQLVLIDYQARLMPAIHDAAAVLANARRLAQMARWLG
                     VPVWATEQNPDKLGGTDAALVPLCDRIVPKMAFGGADALLPLLTPPPAAPRGNARSLP
                     RHLQKAAPPAPERGTVVIAGCETHVCLLQSALLLLEADWDVWVVQDACGSRTERNRDA
                     AYDRLAAAGCELVTTEMVGFEWLRDCQHPQFKAWQGLIK"
     gene            7645..8067
                     /locus_tag="PK242_08955"
     CDS             7645..8067
                     /locus_tag="PK242_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94187.1"
                     /translation="MSSSSKQLRLSRRSSELAQAVPQVMARRVGRMMQAGPVPSMEDQ
                     QEFIRMGMEKLEAFGEAWWAMVLQGSQVQQQFALWWMQTWWKVALGGWMHPPTLSSLS
                     NGASRNLMNGWLDVTLCGVAPVHRRAVANARRLNGSGA"
     gene            8217..>8604
                     /locus_tag="PK242_08960"
     CDS             8217..>8604
                     /locus_tag="PK242_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOZ94188.1"
                     /translation="MTSYADFHRRSIEDRDAFWAEQARLIDWQTPPQTICDYSQPPFA
                     RWFVGGRTNLCHNAVDRHLKDRADQPALIWVSTETDQERVYSFRDLHAEVQRMAAALR
                     ELGVKKGDRVLIYMPMIAEAAFAMLAC"
BASE COUNT         1443 a         3034 c         2917 g         1210 t
ORIGIN      
        1 ggccatgggc gagaccgagc gccgccgcgc caagcagatc gccttcaacg ccgagcaagg
       61 catcaccccg cgcggcatcg tcaagcaggt gcgcgacctg atcgacggcg tctacggcga
      121 acggggtgaa cggcaggtgc tggcgcaggg caggccggcg gtgcaggaaa tcgccctgtc
      181 ggaaaaagac ctggcgcgcg aaatcaagcg gctggaaaag cagatgctgg agcacgcgcg
      241 caacctggag ttcgagcagg cggcgcgggt gcgcgaccag ttgcatgccc tcaagcaggg
      301 cgccttcggc gctcccggca ccgactcgcc gagcgggtga acccggcaca gctgccgtgc
      361 catgcccatg gccaaaaccc cacgattcgt cgggaaatgg tgtatacttg attaaatcac
      421 tcggaattga tcgccggtgc cacggggtaa tcccgaccac tggccccgag tgccagaccc
      481 tcatcgaccg aaggaaccca cgatgcgcct caccaccaaa gggcggtttg ccgtcaccgc
      541 catgatcgat ctggcgctgc gccaggacag cggcccggtc acgctggcgg ccatcagcca
      601 gcgccagcgg atatcgctgt cgtacctgga gcagttgttc ggcaagctgc ggcggcacga
      661 gctggtcgaa tccacgcgcg ggccgggcgg tggctattcg ctgggccgca aggcggacga
      721 catcacggtg gccgacatca tcgtgtcggt cgacgagccg ctggacgcca cccactgcgc
      781 cggcaagcag aactgccagg gcggtgacgg gccgtgcatg acgcacgacc tgtggacggc
      841 gctgaacgac cgcatgctgg acttcctgca gtccgtcacc ctgcagaagc tggtggacga
      901 ccagcacgcc aagggcatcg agatcgaggg caagccggcc accaagcggg ccatctccag
      961 cgcgccggtg gtcaagccga tccgcgtcaa cgcccccaat tcggtcttcg cgctgggcgg
     1021 cctgaccaac aaatagcgaa acgaaccgct ggcgctcgcc cgtcgagggc cagcggcccc
     1081 tccggatccg tttcccgcat gccttcgaga accccacggc catgagccag accccgcatt
     1141 tcccgatcta catggactat ggcgccacca cgccggtcga tccgcgcgtg gtggacgcca
     1201 tggtgccctg gctgcgtgag catttcggca acccggccag ccgcagccac gcctggggct
     1261 gggaggccga acaggccgtc gagaccgcgc gcgagcaggt ggcggcgctg atcggcgccg
     1321 acccgcgcga gatcgtctgg accagcggtg ccaccgagtc cgacaacctc gccatcaagg
     1381 gcgcggcgca gttctacaag ggcaagggca ggcacctgat caccgtcagg accgagcaca
     1441 aggcggtgct ggacgtgatg cgcgaactgg agcgccaggg cttcgaagtc acctacctgg
     1501 aggtgcagcc cgacggcctg ctggacctcg aagccttgcg cgccgccatt cgccccgaca
     1561 ccatcctggc cagcgtgatg ctggtcaaca acgagatcgg cgtgatccag gacgtgcagg
     1621 ccatcggccg cctgtgccgc gaacagggcg tgctcttgca cgtcgatgcc gcccaggcca
     1681 ccggcaaggt ggcgatcgat ctggccacgc tgccggtcga cctgatgagc ctggccagcc
     1741 acaagaccta cggccccaag ggcatcggcg cgctctacgt gcggcgcaag ccgcgcgtgc
     1801 ggctggaagc gcagatgcac ggcggcggtc acgagcgggg catgcgctcg ggcacgctgc
     1861 ccacgcacca gatcgtcggc atgggcgagg cgttccgcat cgcccgcgaa gaaatggcgc
     1921 gcgacagcga aaaggcccgc gccctgcatc ggcgtctggt cgatggcctg aaggacatcg
     1981 aggaggcgta cctcaacggt cacccgacgc agcgcgtgcc ccacaacgtc aacatgagct
     2041 tcaactacgt cgagggcgaa tcgctcatga tgggcatcaa ggggctggcg gtgtccagcg
     2101 gctcggcctg cacctcggcc agcctggagc ccagctacgt gctgcgcgcg ctgggccgca
     2161 gcgacgagct ggcgcactcc tcgctgcgca tcaccatcgg ccgcttcacc accgagcagg
     2221 agatcgacta cgccatccgc gcgatccgcg acaacgtggc gcggctgcgc gagctgtcgc
     2281 cgctgtggga gatgcaccag gacggcatcg acctcagcac catctcgtgg gcggcgcatt
     2341 gaaatttgga tcaaatatgc cattgcggcg cttgcagaaa gcgccggcag ctatgaattc
     2401 aggagtaaac catggcctat tcccaacagg tgattgacca ctacgaaaac ccccgcaacg
     2461 tcggcagctt cgacaagggc gacgccagcg tgggcaccgg catggtgggt gcgccggcct
     2521 gcggcgacgt gatgcgcctg cagatcaagg tcaacccgga caccggcgtg atcgaggacg
     2581 cgcgcttcaa aacctacggc tgcggctcgg cgattgcctc cagctcgctg gtgaccgaat
     2641 gggtcaaggg caagacgctg gcgcaggcca ccgagctgaa gaacagtcag attgccgacg
     2701 aactggcgct gccgccggtc aaaatccatt gctcgatcct ggccgaggac gccatcaagg
     2761 cggcggtcga ggactaccgc aagaagcacg cctgaaaccg gggtgagcgt tcggcgtgcg
     2821 cctggcacgc ctgacgcaag ccttccacct gaacctgcaa actccaacgt atgaccgtca
     2881 ccctgaccga agccgcggcc cggcatgtca gcaaatacct cgcccaacgc ggcaagggcg
     2941 tgggcgtgcg cctgggcgtc aagaccaccg gctgctccgg cctggcctat cagctcgaat
     3001 acgtcgatga agtggcgccc gaagacgcgc tgttcgagca gcacggcgtc aagctcatgg
     3061 tcgatcccaa gagcctggcc tacatcgatg gcaccgaact cgactacgtg cgcgaggggt
     3121 tgaacgaagg ctttcgcttt aacaacccca acgagcgcga ccgctgcggc tgcggggaat
     3181 cgttccgcat ctgacggtct tcgcgggacg ctgcgcgccc gcccggccgt gctcgcttcg
     3241 atgatccagc tgcattccac cgacttcgag ctgttcgacc tgccgccccg cttcgcgcag
     3301 gacggcgcgg tgctggaggc gcgctggaag gcgctgcagc gcgaggtgca tccggaccgc
     3361 tttgccgccc agggcgcggc cgcccagcgc gtggcggcgc agtggtcggc gcgcatcaac
     3421 gaggcccacc ggcggctgaa ggacccgcag cgccgcgccg cctacctgtg cgaactgcac
     3481 ggcgtgccgg tcgacgccga gcgccacacc gccatgccgc cggagttcct gatgcagcag
     3541 atggcgctgc gcgagcagct cgacgaggcc ggcggcgcgc cggcgctgga cgccatcgcg
     3601 gcgcaggtgg ccgggctgcg ccacgagctg ctgcaggctc tggcgcagca acaggacgtg
     3661 gagcgcgact ggagcgccgc cgcccgatcg gtgcgtgcgc tgatgtttct ggacaagctg
     3721 gcggccgacg tcgatcggcg gcgtgacaca ctcgcctgac cccgtcgccc caccgggcgc
     3781 ccaccgacct tcgctgtatg gcgctgctgc agatttccga acccggtcaa tcgcccgacc
     3841 cgcaccagcg gcgcatcgcc gtcggcatcg acctgggcac cacccattcg ctggtggccg
     3901 ccgtgcgcca cggcgtggcc gaatgcctgc ccgacgcgca gggccgcgtg ctgctgcctt
     3961 cggtggtgcg ctaccccgcc gagggcgggc gccagatcgg ccatgccgca gcgtccgacg
     4021 tgccggccga ggccgcgcac accatcggct ccttcaagcg cctgatgggc cgcggcctgg
     4081 gcgacatcgc cggccatgag cggctgccct acccactgct ggaccgtccc ggcatggtgg
     4141 cggtcggcac gccccagggc gagaaatcgc cggtcgagct gagcgccgag attctggcca
     4201 tcctgcgcca gcgcgccgaa gacagcctcg gcggccagct gcacggcgcc gtcatcaccg
     4261 tgccggctta ctttgatgac gcgcagcgcc aggccaccaa ggacgctgca cggctggccg
     4321 gcatcccggt gctgcgcctc atcaacgaac ccacggcggc cgccgtggcc tacggcctgg
     4381 atcgcgccgc cgagggcgtg tatgcggtgt tcgacctcgg cggtggcacc ttcgacctgt
     4441 ccatcatgcg gctgacgcag ggcgtgttcg aagtcgtcgc caccggcggc gacacggccc
     4501 tgggcggcga cgactacgac cacgcgctgg ccgactgggt gctggcgcgc tgcgccgtgc
     4561 aggcccgcac ggcggccgac aaggccgcgc tggcgcgcgc cgcacgccag gccaaggaag
     4621 ccctgagtgc tactgaaaca gtagcttttc gcgcacatct gacaagcggg gaactcgttt
     4681 ttgacctgaa agccgacgac ttcgcccagg ccacccaggc gctcacgcgc cgctgcctgg
     4741 acaccgcgcg ccgtgtgctg cgcgacgccc ggctgacacc ggacgacatc cagggggtgg
     4801 tgctggtggg cggctccacc cgcatgccgg tgatccggca ggcggtggcc gggttgttcg
     4861 gccgcgcgcc gctgaccgac ctcaaccccg acgaggtggt ggccctgggc gccgccatcc
     4921 aggcccagca gctggccggt caccggccgc cgggcgatct gctgctgctg gacgtgatcc
     4981 cgctctcgct ggggctggaa accatgggcg gcctggtcga gcgcatcgtg ccgcgcaacc
     5041 agaccatccc caccgccatg gcgcaggact tcaccaccta ccgcgacggc cagaccgcgc
     5101 tggcgctgca cgtggtgcag ggcgagcgcg acctggtgca ggactgccgc agcctggcgc
     5161 gcttcgagct gcgcggcatc ccgccgatgg ccgccggcgc ggcgcgcatc cgcgtcacct
     5221 tcagcgtcga tgccgatggt ctgctgtcgg tcagcgcccg cgagcagacc agtggcgcgc
     5281 aggcgcagat cgaggtcagg cccagctacg gcctgtccga ccggcagatc gcccagatgc
     5341 tgcaggacag ctttggcacc gccgaagccg acgtgcgggc acgcgccctg gctgaagccc
     5401 gggtcgaggc cgagcgcctg cggctggcca cgcaaggcgc gctggacgcc gatggcgcac
     5461 tgctgagcgc gcccgagcgc cgcgccatcg aggcgctgat gcagacgctg cgcgagcgcg
     5521 ccgccgacgg cagcgacgcc gccgccatcg cagcggccac cgaggcgctg gcccagggca
     5581 ccgaagcctt tgccgcgcgg cgcatgaacg ccggcatccg cagggcgctg accggcagga
     5641 acgtccacag cctctgaccc atgccgacca tccaggttct tccccatccc gagcattgcc
     5701 ccgacggcgc gcgcatccag gcccaggtcg gcgactcgat ctgcgaggcg ctgctggcgc
     5761 acggcatcga gatcgagcac gcctgcgaca tggcgtgcgc ctgcaccacc tgccacgtca
     5821 tcgtgcgcca gggttacgat tcgctggcgt cgcccgagga agaagaggaa gacctgctcg
     5881 accgcgcctg gggcctggag ccgcagtcgc gcctgtcgtg tcaggccgtc gtcgcgcgcg
     5941 ccgacctgac ggtggaaatc ccccgctact cgatcaacca cgccagggaa aagcgctgaa
     6001 ccgcgccgcc tctgcccagc ccacgccgcc tgcccgatga cccgacagat cgttcttgac
     6061 accgaaacca ccggcctgtc ggccgaaagc ggcgaccgca tcatcgagat cggctgcgtc
     6121 gagctggtgg gccgcaagct caccggcaac caccttcatt tctacctcaa cccggagcgc
     6181 gacagccatg aggacgcgct caaggtgcac ggcatcagca acgagttcct gcgcgacaaa
     6241 cccaggttcg ccgaggtggc cgacgagatc ctgcgctatg tcgaaggcac cgagctgatc
     6301 atccacaacg ccgccttcga cctgggtttt ctcgacaagg agctcgagcg cctgggtcgg
     6361 ccgccgctgc gcagcttcgt cgctggcgtc accgacaccc tggccatggc caaacagctc
     6421 tacccgggca agcgcaacag cctggacgcg ctgtgcgagc gcctgggggt ggacaactcc
     6481 ggccgcaccc tgcacggcgc gctgctggac gccgaactgc tggccgacgt ctacatcagc
     6541 ctcacgcgcg ggcaggaggc gctgctgatc gatctgggcg aagtcggcgc cgccggcggc
     6601 gacgccccgc gcatcgatct gaccgggctg gagctgccgg tgctgaccgc caatgcgcag
     6661 gagctggccg cgcacgagga cgtgctggcg cagatcgaca aagccagcgg cggcaagacc
     6721 gtgtggcgac gggtcgaaac cggtctataa tgcgcgcttc gccaaaacgc gaagcgggtg
     6781 attagctcag cggtagagca ctgccttcac acggcagggg tcgcaggttc gaaccctgca
     6841 tcacccacca acatggcaac gccccgcagt ccaacgctac ggggcgtttt tgtttccggc
     6901 gcgtcgcccg cggtggcgcc gcggccagcc aaggagcctg tgcatgctgc tcgacgccga
     6961 cgactgccaa ctggtgctga tcgactatca ggcgcgcctg atgcccgcca tccacgatgc
     7021 cgcggcggtg ctggccaatg cgcgccggct ggcgcagatg gcgcgctggc tgggcgtgcc
     7081 ggtgtgggcg accgagcaga accccgacaa gctgggcggc accgacgcgg cgctggtgcc
     7141 gctgtgcgac cgcatcgtgc ccaagatggc gtttggcggc gccgacgcgc tgctgcccct
     7201 gctgacgccg ccgccggccg cgccgcgcgg caatgcgcgc agcctgccgc gccacctgca
     7261 gaaggctgcg ccacccgcgc ccgagcgcgg caccgtggtc atcgccggct gtgaaaccca
     7321 tgtctgcctg ctgcagagcg cgctgctgct gctggaggcc gactgggacg tctgggtggt
     7381 gcaggacgcc tgcggctcgc gcaccgagcg caaccgcgac gccgcctacg accggctcgc
     7441 cgccgccggc tgcgagctgg tgaccaccga gatggtcggc ttcgagtggt tgcgcgactg
     7501 ccagcacccg cagttcaagg cctggcaggg gctgatcaag tgagcgggga acaccggatt
     7561 gcgtgtcgcc ccgctttcga gtaatttgtg gctccagcgc ttcatgggca agcgccggca
     7621 gctatatttg ttgtagcaat caccatgtcc tcatcgtcca agcaactgcg tctttcgcgc
     7681 cgctccagcg agctggcgca ggccgtgccc caggtcatgg cgcggcgtgt cggccgcatg
     7741 atgcaggccg ggcccgtgcc cagcatggag gaccagcagg aattcatccg catgggcatg
     7801 gaaaagctgg aagcctttgg cgaggcctgg tgggccatgg tgctgcaggg ctcgcaggtg
     7861 cagcagcagt ttgcgctgtg gtggatgcag acctggtgga aggtcgcgct gggcggctgg
     7921 atgcacccgc ccacgctgag cagcctgtcc aacggcgcca gccgcaacct gatgaacggc
     7981 tggctggacg tcaccctgtg cggcgtggcc ccggtgcatc ggcgcgcggt ggccaatgcg
     8041 cggcgcctga acggctcggg cgcctgaggc acgcccgtca gcctgccgca agtccggttt
     8101 ccccataatt gaccgtttgc cgacaggcat cggtgacggg tgggcggcgg ccgggcgcgt
     8161 ggcgcgcgtg gcggtgctgc ccccgtcttg cgacaagggc cgaggagacc agcgccatga
     8221 ccagctacgc cgacttccat cgccgttcca tcgaggatcg cgatgccttc tgggccgaac
     8281 aggcccgtct gatcgactgg cagacgccgc cgcagaccat ctgcgactac agccagccgc
     8341 cgtttgcccg ctggttcgtc ggtggccgca ccaacctgtg ccacaacgcg gtcgaccggc
     8401 acctgaagga ccgcgccgac cagccggcgc tgatctgggt ttccaccgaa accgaccagg
     8461 agcgtgtcta cagcttccgc gacctgcacg ccgaagtgca gcgcatggcg gccgcgctgc
     8521 gcgagctggg ggtgaagaag ggcgaccggg tgctgatcta catgcccatg atcgccgagg
     8581 ccgccttcgc catgctggcc tgca
//
LOCUS       DAOOOG010000111         8462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_9831_length_8462_cov_26.666231, whole genome shotgun sequence.
ACCESSION   DAOOOG010000111 DAOOOG010000000
VERSION     DAOOOG010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9831_length_8462_cov_26.666231"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PK242_08965"
     CDS             <1..1067
                     /locus_tag="PK242_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ94189.1"
                     /translation="NQMLVEMDGFETNLGVIVVAATNRPDILDAALLRPGRFDRQVYV
                     TLPDIRGREQILNVHMRKIPIGQDVSPAIIARGTPGMSGADLANLCNEAALMAARRNA
                     RVVEMQDFEKAKDKILMGPERKSMVMPEEERRNTAYHESGHALIGKLLPKCDPVHKVT
                     IIPRGRALGVTMSLPENDRYSYDKQYMLNQISMLFGGRIAEEVFMNQMTTGASNDFER
                     ATQIARDMVMRYGMSEAMGPMVYAENEGEVFLGRSVTKTTNISEETMQKVDAEVRRII
                     DEQYALARKLIEDNQDKMHAMAKALLEWETIDGEQLDDIMAGREPRPPKDFTPRTPPS
                     GGTGGTPAEAKPADPAPTAA"
     gene            1253..2110
                     /gene="folP"
                     /locus_tag="PK242_08970"
     CDS             1253..2110
                     /gene="folP"
                     /locus_tag="PK242_08970"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOZ94190.1"
                     /translation="MIWQTSRFRIDLAEPRVMGIVNVTPDSFADGGQHPDAASALRHC
                     ERLLKDGAQLLDIGGESTRPGSPPLPLAEELARVLPVLRGAVQLGVPVSIDTYKPEVM
                     QAALDLGVDIVNDVWALRQPGAAQLVARHPSCGIVLMHMHGEPRSMQTAPMEGDAPPQ
                     VLSFLEHAAQGLQALGVDRTRIVVDPGIGFGKTVAQNFSLLARQRELLAAGWPLLVGW
                     SRKSSIGAALQGDAPAPPPPAERVLGSVAAALLAVERGARIVRVHDVRETVQALAVWR
                     QARQTLLKS"
     gene            2172..3533
                     /gene="glmM"
                     /locus_tag="PK242_08975"
     CDS             2172..3533
                     /gene="glmM"
                     /locus_tag="PK242_08975"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOZ94191.1"
                     /translation="MSRQYFGTDGIRGTVGQAPITPDFALRLGHAVGRVLRRTQPDAT
                     VLIGKDTRISGYMLESALESGFNSAGVDVVLLGPVPTPAVAYLTRAQRAELGVVISAS
                     HNPYPDNGIKFFSARGTKLPDDWEREVEAVLAEVPQWVSADALGRARRLDDAAGRYIE
                     FCKSTFANDLTLRGLKIVVDAAHGAAYQIAPKVFHELGADVLAIGCAPDGLNINDAVG
                     ATHPEALVRAVAEHGADYGIALDGDADRLQMVDASGRLFNGDELLYVLAIDRLARDAS
                     LRGAGAPRPPALAGVVGTLMTNLAVEQALRARGFDFVRAKVGDRYVLEELVQRGWILG
                     GEGSGHLLALDKHTTGDGLISALQVLQACAMQGRTLAQLLEGVALCPQVLINVRLQAG
                     QDWRHYTRLTDATRAVEAELGASGRVLIRASGTEPLLRVMVEAVDAAQAQRCAERLAA
                     AAG"
     gene            complement(3658..4482)
                     /locus_tag="PK242_08980"
     CDS             complement(3658..4482)
                     /locus_tag="PK242_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOZ94192.1"
                     /translation="MNTQDILHFITTQGAEFGLKLLTAIAAWIVGRWLIGLAVGLMGK
                     IFERGGKIDTTLAHYLKSITSVLLNIVLILAILDIFGVKTTSFAALLAGAGLAIGAAW
                     SGMLGNFAAGVFMQVLRPYKVGDFISAGGVVGTVTELGLFATTLTTPDNVMTVVGNGK
                     IFGDNIQNFSALPVRRVDCVAKVANSVNPLDAMARLKPVIAAIPNVAASPAPDIEILE
                     FTPEGPKLCVRPYTHTDHYWQVYFDTHKAIVETFGAAGYPVPETPVAYRSAPSGVA"
     gene            4671..5429
                     /locus_tag="PK242_08985"
     CDS             4671..5429
                     /locus_tag="PK242_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HOZ94193.1"
                     /translation="MLKRQQPTFSEVNLSEHDGVRYLHLGTEWVQGSMRIDAPFEIDL
                     EYVQRMMAWLLFVPEDSVAGRHAMQLGLGAGAITKFCYKKLRMTCTAVELNPRVADVC
                     RAWLRLPPDDERLRVVLADAAEEIRRDEWLGTVDALAVDLYDDEAAAPVLDSAAFYAD
                     CRRLLTEDGCMTVNLFGRSSSFERSLACISEAFGAQAVWAFKATREGNTVVLAQRSAT
                     RPKRPLLQQRAEEIEARWGLPARKWLKVFKAIEK"
     gene            5426..7240
                     /locus_tag="PK242_08990"
     CDS             5426..7240
                     /locus_tag="PK242_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOZ94194.1"
                     /translation="MKRSTRPTPLTVVDGYRGPVHWRLLLQWLREDAVISAAEAARVH
                     ARCAQAESAQHALVRLAAVGITRAADGAAMDVEALTRYLAERAGLAYLRIDPLRVDVG
                     KVSDTMSASYAERHKVLPVQVTPAEVVVATSEPFIDDWVAEVERQSRRQVRRVVANPL
                     DIKRYTAEFFALARSVRVAQKQGGGSAGAASFEQLVELGRTGRQLDANDQGVIQVVDW
                     LWQYAFDQRASDIHLEPRREQGVIRFRIDGVLHPVYQVPAGVMNAITARIKLLGRMDV
                     VERRRPQDGRIKTRSPGGEEIEMRLSTLPTAFGEKLVMRIFDPDTAVKDLAALGFTPH
                     DAARWEALVQRPHGIILVTGPTGSGKTTTLYSTLRRVATEEVNVSTIEDPIERIDPAL
                     NQTQVQPQLDFSFAEGVRALMRQDPDIIMIGEIRDLETAEMAVQAALTGHLVFSTLHT
                     NDAPSALTRLMELGVPPYLLSATILGVLAQRLVRTLCPHCRQPDEQGSQPLLADVIKP
                     WKINAADYRPYKPVGCVDCRMTGFRGRMGIYELLTVSDAFKDRVRADVDLAALRQQAI
                     ADGMRPLRLAGALRAAEGLTTLQEVIAATPPLEPRAHS"
     gene            7444..7932
                     /locus_tag="PK242_08995"
     CDS             7444..7932
                     /locus_tag="PK242_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOZ94195.1"
                     /translation="MRIKSQRDFFAGLLYVVIGGAFAIGANNYTIGNAARMGPGYFPL
                     LVGILLAILGAIIVVKSLVVETPDGDPIGPWAWKPLIYIILANFLFGLLLGGVPSLGI
                     PSLGVMIGILVLTLVAAKAGPEFKLKEVIILAIVLAAGSWLVFIWGLNLQMPVWPSFI
                     SG"
     gene            7952..>8462
                     /locus_tag="PK242_09000"
     CDS             7952..>8462
                     /locus_tag="PK242_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOZ94196.1"
                     /translation="MELLEHLALGFSVAFTLQNLMYAFIGCLLGTLIGVLPGVGPVAT
                     IAMLLPTTYALPPVAALIMLAGIYYGAQYGGSTTAILVNLPGEASSVVTVIDGHQMAR
                     KGRAGPALAAAAIGSFIAGCFGTLVLAGFAVPLTEVAFKFGPAEYFSLMVLGLIGAVV
                     LASGSLIKAV"
BASE COUNT         1332 a         2899 c         2925 g         1306 t
ORIGIN      
        1 tgaaccagat gctggtcgag atggacggct tcgagaccaa cctgggcgtg atcgtggtgg
       61 ctgccaccaa ccgccctgac atcctggacg ccgccctgct gcgccccggc cgctttgacc
      121 gccaggtcta cgtgacgctg cccgacatcc gcggccgcga gcagatcctg aacgtgcaca
      181 tgcgcaagat cccgatcggc caggacgtca gccccgccat catcgcgcgc ggcacgcccg
      241 gcatgagcgg tgccgatctg gccaacctgt gcaacgaggc cgcgctgatg gccgcgcgcc
      301 gcaacgcgcg cgtggtcgag atgcaggact tcgaaaaggc caaggacaag atcctgatgg
      361 gccccgagcg caagagcatg gtcatgcccg aagaggagcg ccgcaacacc gcctaccacg
      421 agagcggcca cgccctgatc ggcaagctgc tgcccaagtg cgacccggtg cacaaggtca
      481 ccatcatccc gcgcggccgg gcgctgggcg tgaccatgag cctgcccgag aacgaccgct
      541 acagctacga caagcagtac atgctgaacc agatcagcat gctgttcggt ggccgcatcg
      601 cggaagaggt gttcatgaac cagatgacca ccggcgccag caacgacttc gagcgcgcca
      661 cccagatcgc gcgcgacatg gtgatgcgtt acggcatgag cgaggccatg gggcccatgg
      721 tgtatgccga aaacgagggc gaggtgttcc tgggccgcag cgtcaccaag accaccaaca
      781 tctcggaaga gaccatgcag aaggtcgatg ccgaggtgcg tcgcatcatc gacgagcagt
      841 acgcgctggc gcgcaagctg atcgaggaca accaggacaa gatgcacgcc atggccaagg
      901 cgctgctgga atgggaaacc atcgacggcg agcagctcga cgacatcatg gccgggcgcg
      961 agccgcgtcc gcccaaggac ttcacgccac gcacgccgcc ctcgggcggc accggcggca
     1021 ccccggccga agccaagccg gccgatccgg caccgacagc agcctgattg ggccgctgac
     1081 gaacccatcg aacccccgta cgccaaggac gcaaagcttg cgccaaagac gcaaaagaac
     1141 cgcccatcca ggtttttctg cgttctctgc gaattctttg catcctccgc gtagggaggt
     1201 tcaggatttt ctggtttcta cggggccgcg gccccgtttc tattgacgtc gcatgatctg
     1261 gcagaccagc cgctttcgca tcgacctggc cgagccgcgg gtgatgggca tcgtcaacgt
     1321 cacgcccgac tcgtttgccg acggcggcca gcacccggac gccgcctcgg ccctgcgcca
     1381 ctgcgagcgc ctgctgaagg acggggcgca gctgctggac atcggcggcg aatcgacccg
     1441 ccccggcagc ccgccgctgc cgctggccga agagctggcg cgcgtgctgc ccgtgttgcg
     1501 cggtgccgtg cagttgggcg tgccggtgtc gatcgacacc tacaaacccg aggtcatgca
     1561 ggccgcgctg gatctgggcg tggacatcgt caacgacgtc tgggcgctgc gccagcccgg
     1621 cgcggcccag ctcgtggcgc gccacccgtc ctgcggcatc gtgctgatgc acatgcatgg
     1681 cgagccgcgc agcatgcaga ccgcccccat ggagggcgac gcgccgcccc aggtgctctc
     1741 gtttctggag catgcggcgc agggcctgca agcgctgggc gtcgacagga cccgcatcgt
     1801 ggtggacccg ggcatcggct tcggcaagac cgtggcgcag aacttcagcc tgctggcgcg
     1861 ccagcgcgag ctgctggcgg cgggctggcc gctgctggtc ggctggtcgc gcaagtccag
     1921 catcggcgca gcactgcagg gcgatgcgcc ggcgccgcca ccgcctgccg agcgcgtgct
     1981 gggcagtgtg gcggcggcgt tgctggcggt cgagcgcggc gcccgcatcg tgcgcgtgca
     2041 tgatgtgcgc gaaaccgtgc aggcgctggc ggtgtggcgc caggcccgcc agacactatt
     2101 aaaatcatag ctgctggcgc ttgccctgcg ggcgcaagcg ccgtttttct ttctaaggag
     2161 ggagcttggg catgagccga cagtactttg gcaccgatgg cattcgtggc accgtggggc
     2221 aggccccgat cacgccggat ttcgcgctgc gcctgggcca cgcggtcggg cgcgtgctgc
     2281 gacgcacgca gcccgacgcc acggtgctga tcggcaagga cacgcgcatt tcgggctaca
     2341 tgctggagag cgcactggaa tcgggcttca actcggccgg cgtcgacgtg gtgctgctcg
     2401 ggccggtacc cacgccggcc gtggcctacc tgacgcgtgc ccagcgcgcc gagctggggg
     2461 tggtgatcag cgccagccac aatccctacc ccgacaacgg catcaagttc ttcagcgccc
     2521 gcggcaccaa gctgcccgac gactgggagc gcgaagtcga ggccgtgctg gccgaggtgc
     2581 cgcagtgggt cagtgccgat gccctggggc gcgcgcgccg gctcgacgac gccgccggcc
     2641 gctacatcga gttctgcaag agcacttttg ccaacgacct gacgctgcgc ggactgaaga
     2701 tcgtggtcga tgcggcgcat ggcgccgcct accagatcgc gcccaaggtg ttccatgaac
     2761 tgggcgccga cgtgctggcc atcggctgcg cgcccgacgg cctgaacatc aacgacgccg
     2821 tcggcgccac ccaccccgag gcgctggtgc gcgccgtggc cgaacacggc gccgactacg
     2881 gcattgcgct ggacggcgac gccgaccgcc tgcagatggt cgacgccagc ggccgcctgt
     2941 tcaacggcga cgaactgctg tacgtgctgg cgatcgaccg gctggcgcgc gacgccagcc
     3001 tgcgcggcgc gggcgcgccg cgcccgccgg cgctggcagg cgtggtcggc accctgatga
     3061 ccaacctggc ggtcgagcag gcgctgcgcg cgcgtggttt cgacttcgtg cgcgccaagg
     3121 tgggcgaccg ctacgtgctg gaagagctgg tgcagcgcgg ctggattctg ggcggcgaag
     3181 gttcgggcca cctgctggcg ctggacaagc acaccaccgg cgacggcctg atctccgccc
     3241 tgcaggtgct gcaggcctgc gccatgcagg ggcgcacgct ggcgcagctg ctcgagggtg
     3301 tggcgctgtg cccgcaggtg ctgatcaacg tgcgcctgca ggccggtcag gactggcgcc
     3361 actacacccg cctgaccgac gccacgcgcg ccgtcgaagc cgaactgggc gcgagcggcc
     3421 gggtgctgat ccgcgccagc ggcaccgagc cgctgctgcg cgtgatggtc gaggcggtgg
     3481 atgccgccca ggcccagcgc tgcgccgagc ggctggcggc ggcggcgggc tgacgcaacg
     3541 gatgcgtcat ccccgcgaca gcggggatga cgggcccttc ttcgacctct ttcgcgtcct
     3601 gcgcgaatcc ttggcgactt ctgcttccgg cggttgggtt gttctgcacg ccggccgtca
     3661 cgccaccccg gaaggcgcgc tgcgataggc caccggcgtc tccggcaccg gatagccggc
     3721 ggcgccaaag gtctcgacga tggccttgtg ggtgtcgaag tacacctgcc agtaatggtc
     3781 cgtgtgggtg taggggcgca cgcagagctt ggggccttcg ggcgtgaatt ccaggatctc
     3841 gatgtccggc gcgggcgagg cggccacgtt gggaatcgcg gcgatcacgg gcttgaggcg
     3901 cgccatcgcg tccagcgggt tcacgctgtt ggccaccttg gccacgcagt ccacgcggcg
     3961 caccggcagg gcgctgaagt tctggatgtt gtcgccaaag atcttgccgt tgccgaccac
     4021 ggtcatcacg ttgtcgggcg tggtcagcgt ggtggcgaac aggccgagct cggtcaccgt
     4081 gcccaccacg ccgccggcgc tgatgaagtc gcccaccttg taggggcgca gcacctgcat
     4141 gaacacgccc gcggcaaagt tgcccagcat gccgctccag gccgcgccaa tggccaggcc
     4201 cgcgccggcc agcagcgcgg caaacgaggt ggtcttgacg ccgaagatgt ccaggatcgc
     4261 caggatcagg acgatgttca gcagcaccga ggtgattgac ttcaggtagt gcgccagcgt
     4321 ggtgtcgatc ttgccgccgc gctcgaagat cttgcccatc aggccgaccg ccagcccgat
     4381 caaccagcgg ccgacgatcc aggcggcgat ggcggtcagc agcttgaggc cgaactcggc
     4441 gccctgggtg gtgatgaaat gcaggatgtc ctgggtgttc atggtggttg ctcgcaatgc
     4501 gcgggtgaat gtccgaggcg gcgactttgc cacgacttcg cccgcgccgc cggcgcccac
     4561 ggtctgcgcc gtgatgcatg tcacgtcgca gggtgcccgc agaccgtgtg cgcggcctgc
     4621 gggcgggcaa accgccccgg cgcgggcacc tgcggtgata acctccgtcc atcttgaaac
     4681 gccagcagcc caccttcagc gaagtcaacc tgtccgaaca cgacggcgtg cgatacctgc
     4741 acctgggcac cgaatgggtg caggggtcga tgcgcatcga cgcgccgttc gagatcgacc
     4801 tggaatacgt gcagcgcatg atggcctggc tgctgttcgt gccggaagac agcgtggccg
     4861 ggcgccatgc catgcagctg ggtctggggg caggggcgat caccaagttc tgctacaaga
     4921 agctgcgcat gacctgcacc gcggtggagc tgaacccgcg cgtggccgac gtctgccgcg
     4981 cctggctgcg cctgccgccc gacgacgagc gcctgcgcgt ggtgctcgcc gacgccgccg
     5041 aagaaatccg ccgcgacgag tggctgggca cggtcgacgc gctggcggtc gatctgtacg
     5101 acgacgaggc cgccgcgccg gtgctggatt cggccgcttt ctatgccgac tgccggcgcc
     5161 tgctgaccga agacggctgc atgacagtca acctgttcgg ccgctccagc agcttcgaac
     5221 gcagcctggc atgcatcagc gaagccttcg gcgcccaggc ggtgtgggcc ttcaaggcca
     5281 cgcgcgaggg caacaccgtg gtgctggccc agcgcagcgc aacgcgcccc aagcgtccgc
     5341 tgctgcagca gcgcgccgaa gagatcgagg cacgctgggg cctgccggcg cgcaaatggc
     5401 tgaaggtttt caaggcgatc gagaaatgaa gcgttccacc cgccctaccc ccctgaccgt
     5461 cgtcgacggc taccgtggcc cggtgcactg gcgcctgctg ctgcaatggc tgcgcgagga
     5521 cgcggtcatc agcgccgccg aagccgcccg cgtgcatgcg cgttgcgcgc aggccgagag
     5581 tgcgcagcac gccctggtgc ggctggcggc ggtgggcatc acgcgcgctg ccgacggcgc
     5641 cgccatggac gtggaagcgc tgacgcgcta cctggccgaa cgcgccggcc tggcctactt
     5701 gcgcatcgac ccgctgcggg tggacgtggg caaggtgtcc gacaccatga gcgccagcta
     5761 cgccgaacgc cacaaggtgc tgccggtgca ggtcacgccg gccgaagtgg tggtggccac
     5821 cagcgagccc ttcatcgacg actgggtggc cgaggtcgag cgccaatcgc gccggcaggt
     5881 gcggcgcgtg gtggccaacc cgctcgacat caagcgctac acggccgagt tctttgccct
     5941 cgcccggtcg gtgcgggtgg cgcagaagca gggtggcggc agcgccggcg ccgccagctt
     6001 cgagcagctg gtcgagctgg gtcgcaccgg tcgccagctc gacgccaacg accagggcgt
     6061 gatccaggtg gtggactggc tgtggcaata cgccttcgat cagcgcgcca gcgacatcca
     6121 tctggagccg cggcgcgagc agggcgtgat ccgctttcgc atcgacggcg tgctgcaccc
     6181 ggtctatcag gtgcccgccg gcgtgatgaa cgccatcacc gcgcgcatca agctgctggg
     6241 ccgcatggac gtggtcgagc gccgccggcc gcaggacgga cgcatcaaga cgcgcagccc
     6301 gggcggcgaa gaaatcgaga tgcgcctgtc caccctgccc accgccttcg gcgaaaagct
     6361 ggtgatgcgc atcttcgacc ccgacaccgc cgtcaaggac ctggcggcgc tgggcttcac
     6421 cccgcacgac gcggcgcgct gggaagcgct ggtgcagcgc ccgcacggca tcattctggt
     6481 gaccgggccg accggctcgg gcaagaccac cacgctgtac tccaccctgc gccgcgtggc
     6541 gacggaggag gtcaacgtca gcaccatcga ggacccgatc gagcgcatcg acccggcgct
     6601 caaccagacc caggtgcagc cgcagctcga cttcagcttc gctgaaggcg tgcgcgccct
     6661 gatgcggcag gacccggaca tcatcatgat cggcgagatc cgcgacctgg agaccgccga
     6721 gatggcggtg caggccgcgc tcaccggcca tctggtgttc agcaccctgc acaccaacga
     6781 tgccccttcg gcgctgacgc gcctgatgga gctgggtgtg ccgccctacc tgctgtcggc
     6841 caccatcctg ggggtgctgg cgcagcgcct ggtgcgcacc ctgtgcccgc actgccgcca
     6901 gccggacgag cagggttcgc agccgctgct ggccgatgtc atcaaaccct ggaagatcaa
     6961 cgccgccgac taccgcccgt acaagccggt gggctgcgtg gactgccgca tgacgggctt
     7021 tcgcggtcgc atgggcatct acgaactgct caccgtcagc gacgccttca aggaccgggt
     7081 gcgtgccgat gtcgacctgg cggcgctgcg ccagcaggcg atcgccgacg gcatgcgccc
     7141 actgcggctg gccggtgcgc tgcgcgccgc cgaaggcctg actacgctgc aggaggtgat
     7201 cgccgccacg ccgccgctgg agccgcgcgc ccactcctga gttgccgggc gtatcggccg
     7261 ccgcgcccgc cgaaatggcg cagcatgctg cgtccaccgc agccatcggg ggcgtctgca
     7321 tgccaccgtc cccctcgtgc gccctagggt aatcccaggg gcggtctagg tggaatccac
     7381 atcgaccggc gcccatgcgc tggtgacaat gggcgctggg ttttgcactc caggagaaga
     7441 ttcgtgcgca tcaaaagtca gagagacttc tttgccgggc tgctgtatgt ggtcatcggc
     7501 ggcgcctttg ccatcggcgc caacaactac acgatcggca acgccgcccg catggggccg
     7561 ggctactttc cgctgctggt gggcattctg ctggccattc tgggcgccat catcgtggtc
     7621 aagtcgctgg tggtggaaac gcccgacggc gacccgatcg gcccgtgggc ctggaagccg
     7681 ctgatctaca tcatcctggc caacttcctg ttcggcctgc tgctgggcgg cgtgccttcg
     7741 ctgggcattc cgtcgctcgg ggtgatgatc ggcatcctgg tgctcacgct cgtggcggcc
     7801 aaggcggggc cggagttcaa gctcaaggaa gtgatcatcc tggccatcgt cctggccgcc
     7861 ggcagctggt tggtcttcat ctggggcctg aacctgcaga tgccggtctg gccgagcttc
     7921 atttcgggct gagtcgcagg gagcacacac catggaattg ctggaacacc ttgcccttgg
     7981 cttcagcgtg gccttcacgc tgcagaacct catgtacgcc ttcatcggct gcctgctggg
     8041 cacgctgatc ggcgtgctgc cgggcgtcgg cccggtggcg accatcgcca tgctgctgcc
     8101 caccacctac gcgctgccgc cggtggcggc gctgatcatg ctggccggca tctattacgg
     8161 cgcgcagtac ggcggctcga ccaccgccat cctggtcaac ctgccgggcg aggcctcgtc
     8221 ggtggtgacg gtgatcgacg gccaccagat ggcgcgcaag ggccgcgccg gcccggcgct
     8281 ggctgcggcc gccatcggct cgttcatcgc cggctgtttc ggcaccctgg tgctggccgg
     8341 ctttgccgtg ccgctgaccg aggtggcctt caagttcggc ccggccgaat acttctcgct
     8401 catggtgctg ggcctgatcg gcgccgtggt gctggcttcc ggttcgctga tcaaggccgt
     8461 cg
//
LOCUS       DAOOOG010000112         8442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_9857_length_8442_cov_31.824133, whole genome shotgun sequence.
ACCESSION   DAOOOG010000112 DAOOOG010000000
VERSION     DAOOOG010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9857_length_8442_cov_31.824133"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="PK242_09005"
     CDS             complement(<1..1199)
                     /locus_tag="PK242_09005"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOZ94197.1"
                     /translation="MQSVKTELLAALAAELERLWPGSGSKAAFESPRQAAHGDFACTA
                     AMQLAKPLKANPRALGEQLQQALQAAPAFQRWVSAIELAGPGFLNIRLQPAAKQQVVL
                     EVLSQGARYGSRPARGEKMLVEFVSANPTGPLHVGHGRQAALGDAICHLFTSQGWQVQ
                     REFYYNDAGVQIDTLATSVQCRARGLKPGDADWPEAAYNGDYIQDIADDFLARRTVQA
                     DDRQFTASGSVDDLDGMRQFAVAYLRHEQDKDLQAFRLKFDRYYLESSLYAEGRVEAT
                     VQRLIDAGKTYEEGGALWLRSTDYGDDKDRVMRKSDGGYTYFVPDVAYHITKWERGFR
                     KAINIQGTDHHGTIARVRAGLQAAGVGIPAGYPDYVLHTMVRVVRGGQEVKISKRAGS
                     YVTLRDL"
     gene            complement(1331..1780)
                     /locus_tag="PK242_09010"
     CDS             complement(1331..1780)
                     /locus_tag="PK242_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HOZ94198.1"
                     /translation="MLEAWLAFFHISAVIGWVVFASAQAAICRAEWLNATVVQRLARL
                     DRILWIATAAVLLSGLARICWGAKGAAWYWSNPLLHLKLTLFLVAAVLMLGPTRRIAR
                     WQRALAADASLPAAHEVQGVRRLVMIETHLVAVIPLAAVFLARGYGA"
     gene            complement(1811..2800)
                     /locus_tag="PK242_09015"
     CDS             complement(1811..2800)
                     /locus_tag="PK242_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ94199.1"
                     /translation="MDKLKAFETFASVATRGSLSAAARAEGVAPAVVSRRLDALEEHL
                     GVKLLVRTTRRIALTHEGTAFLDDCQRILAEVAGAEASVSAGGVQASGHLRVTAPAGF
                     GRRHVAPLVPEFHALHPRLRISLNLSDRVVDLAAEGYDCAVRVGDLPDSSLISIRLAD
                     NQRLCVAAPAYLARRGRPRHPRELAQHDCLMLSSDASQSRGWAFRVPRQAHGQNDERP
                     RAGAGVPIQRSDVEYDVIHLRPEGRLDCTDGQVLHDWCLAGHGIAWRSTWEVEHAIAS
                     GRLVPVLEAFAAPPNGIYAIVPHPTRHLPLRVRLWVDHLRQRYGDPAFWRRPG"
     gene            2911..3609
                     /locus_tag="PK242_09020"
     CDS             2911..3609
                     /locus_tag="PK242_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94200.1"
                     /translation="MRAESAPPIDRRHIDVRTMSLAQLQKLAERGSRRARAELEARMR
                     APGPVLLPASVPADRTAPSAPSRHPPTLTARADPPPQATAAGAAAPAPGAVPAGQGVP
                     VSADALAQQLHLIARQDEAHARAAGPPRLVGLVLTAWGVLLLLGGLLMLGRGGAALYY
                     LFCGLGAAAVGALLMRRSRWALALHGALLPLALGWAWAGAGRHSLPLSVVQAGPLLIP
                     ALWMALRQVREALE"
     gene            3861..6080
                     /locus_tag="PK242_09025"
     CDS             3861..6080
                     /locus_tag="PK242_09025"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HOZ94201.1"
                     /translation="MTARTRIHDLHVADTLYEFINREVLPDVGVDAARFWQGFSAIVT
                     ELAPKNIALLAERDRLQKELDTWHQANPGPIKDMRAYRAFLERIGYLVPQPAKVKVTT
                     KRVDAELAVQAGPQLVVPILNARYALNAANARWGSLYDALYGTDVIPETKGLEKGAKY
                     NPRRGAKVIEWARHVLDRTAPLKKGSHVDSVAYLVRDGELVVKLKDGKSSKLADPAQF
                     IGYQGEPKAPSSILLLHHGLHLDIRIDPASPIGRNDPAGVADLVVEAAVSTILDLEDS
                     IAAVDAEDKVLGYRNWLGILRGTLTESFDKGGKTLTRGLNPDRLYTAASGKGQVRLHG
                     RSLMFVRNVGHLMTNPAILYRAADGSQREIPEGIMDAVVTTTIALHDLQRKGANGIRN
                     SRTGSVYIVKPKMHGPAEVGFAAELFGRVEQLLGLADSTVKLGIMDEERRTSVNLKAC
                     IAAAKSRVAFINTGFLDRTGDEMHSCMRAGPVYRKGDMKTSAWIQAYERNNVLVGLAC
                     GLRGKAQIGKGMWAMPDLMKAMLEQKIAHPRAGANTAWVPSPTGATLHAMHYHQVLVS
                     EVQQRLEAELAQADPAALRDELLTGLLTIPVVSEDKWTAAEKQQELDNNAQGILGYVV
                     RWVDQGVGCSKVPDIHNVGLMEDRATLRISSQHMANWLLHGVVSEGQVRATLQRMAAL
                     VDQQNGGDPLYRRMHDRFEQSAAFQAACDLVFKGVQQPSGYTEPLLHAWRQKVKAGA"
     gene            complement(6198..6581)
                     /locus_tag="PK242_09030"
     CDS             complement(6198..6581)
                     /locus_tag="PK242_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOZ94202.1"
                     /translation="MSQTLTPAQLEQLKQRLLARRQELYAQMQQNRANLAPAENTAGS
                     VSQDENARLANQTREVDTRLTALDAEELARIDRALEAMDAGDYGRCDECGCQIPFERL
                     QIEPMTQHCVACKSAWEHKQGLARA"
     gene            6660..6875
                     /locus_tag="PK242_09035"
     CDS             6660..6875
                     /locus_tag="PK242_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HOZ94203.1"
                     /translation="MPLSAPPIDPTRCPLCGQPNRCAQQARPAGAPAAPHCWCMERRV
                     APDALARVPAQARGRACLCPACATPRA"
     gene            6890..8359
                     /locus_tag="PK242_09040"
     CDS             6890..8359
                     /locus_tag="PK242_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ94204.1"
                     /translation="MHDPPPSSSAEALRARHGARWRWLLLLTVMVGTMASIMSSTVVN
                     VAIPGLSQQFTLGQARAQWVSSGFMVAMTVSMLTTPWLLARFGYRATYNGCMLLLLAG
                     GVLGGVAQSYPLVLAARVAEGLAAGVVQPIPAVIIMRAFEPNEQGRASGLFGMGVVLA
                     PALGPSIGGVLVDWFGWRSIFFMVAPFCLLALWLARRYVPTTAPGGAATRRDETLDGV
                     GLLLGSAGTLCLLNGLVALRAGPAVEAAALLALAAICLAGFIGWQRRCVRLGRAPLMN
                     LALFAWRPFAMGVLVALIYGTALFGSTYLLPVYMQMGLGLSASSVGAILLPAGLVLAA
                     TIPLVARWADRLPASGLVNTGLALLALSFAAMVTVGLAPGLWHLTALGIVGRIGLGFI
                     LPSLNLGAMRGLALPLIPQGSSVINFARTLGGAAGISLCGIALEWRLAAHGTSLTEGA
                     PSAARLAAFDEVFLMLAALCGMAMLAARHLSTPAAGADR"
     gene            8370..>8442
                     /locus_tag="PK242_09045"
     CDS             8370..>8442
                     /locus_tag="PK242_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOZ94205.1"
                     /translation="MCQLLGMNCNTPTDVSFSFSGFAQ"
BASE COUNT         1181 a         2966 c         3038 g         1257 t
ORIGIN      
        1 atcaggtcgc gcagcgtgac gtagctgccg gcgcgcttgc tgatcttgac ctcctggccg
       61 ccgcgcacca cgcgcaccat ggtgtgcagc acgtagtcgg ggtagccggc cggaatgccg
      121 acgccggcgg cctgcaggcc ggcgcgcacg cgcgcgatgg tgccgtggtg gtcggtgccc
      181 tggatgttga tggctttcct gaagccgcgt tcccacttgg tgatgtggta ggccacgtcg
      241 ggcacgaaat aggtgtagcc gccgtcggac ttgcgcatca cgcggtcctt gtcgtcaccg
      301 tagtcggtgc tgcgcagcca cagagcgccg ccttcctcgt aggtcttgcc ggcgtcgatc
      361 aggcgctgca cggtggcttc gacgcgtcct tcggcgtaca ggctggactc caggtagtag
      421 cggtcgaact tgaggcgaaa ggcctgcagg tccttgtcct gctcgtggcg caggtaggcg
      481 acggcgaact ggcgcatgcc gtccaggtcg tccacgctgc cgctggcggt gaactggcgg
      541 tcgtcggcct gcaccgtgcg gcgggccaga aaatcgtccg cgatgtcctg gatgtagtcg
      601 ccgttgtagg ccgcctcggg ccagtcggcg tcgcccggtt tcaggccgcg ggcgcggcat
      661 tgcaccgagg tcgccagggt gtcgatctgc accccggcgt cgttgtaata gaactcgcgc
      721 tgcacctgcc agccctgact ggtgaacagg tggcagatgg cgtcgcccag cgccgcctgg
      781 cggccgtggc cgacgtgcag cgggccggtg gggttggcgc tgacgaactc gaccagcatc
      841 ttctcgccgc gcgccgggcg gctgccgtag cgcgcgcctt ggctcagcac ctcgagcacc
      901 acctgctgct tggcggcggg ctgcaggcgg atgttcagaa agccggggcc ggccagctcg
      961 atcgccgaca cccagcgctg aaaggccggc gcggcctgca acgcttgctg aagctgctcg
     1021 cccagcgcgc gcgggttggc cttgagcggc ttggccagct gcatggcggc ggtgcaggcg
     1081 aagtcgccat gcgcggcctg acggggcgat tcaaaggcgg ctttcgaccc gctgccgggc
     1141 cacagccgct ccagctcggc cgccagcgcg gcgagcagtt cggtcttcac ggattgcatc
     1201 ggcggatttt acggggcggg cgccgggccc cggggtgtgc cgcccaggcc cctgggtgat
     1261 gcatcgaatc gggctctggc gctcatccag caagcgccac aagctatgat aaaaatagca
     1321 aatgccggct tcacgcgccg taaccacggg ccaggaacac cgccgccagc ggaatcaccg
     1381 ccaccaggtg ggtctcgatc atcaccaggc ggcgcacgcc ctgcacctcg tgcgctgccg
     1441 gcaggctggc gtcggccgcc agcgcccgct gccagcgtgc gatgcgccgc gtcggcccca
     1501 gcatcagcac ggcggcgacc aggaacagcg tcaacttcag gtgcagcagc gggttgctcc
     1561 agtaccaggc ggcgcccttg gcgccccagc agatgcgagc cagcccggac agcagcacgg
     1621 cggcggtggc gatccacaga atgcggtcca gccgcgccag ccgctggacc acggtggcgt
     1681 tgagccattc ggcgcggcag atcgccgcct gcgcggacgc gaacaccacc cagccgatca
     1741 cggcgctgat gtgaaagaag gcaagccagg cttcgagcat ggcccggatc gtacccgggc
     1801 cgcccaggcg tcagcccggc cggcgccaga aggccggatc gccgtagcgc tggcgcaggt
     1861 gatcgaccca cagccgcacg cgcagcggca ggtggcgcgt ggggtgcggc acgatggcgt
     1921 agatgccgtt gggcggcgcc gcgaacgcct ccagcaccgg caccagccgg ccgctggcga
     1981 tggcgtgctc gacttcccag gtcgagcgcc aggcgatgcc gtggccggcc aggcaccagt
     2041 cgtgcagcac ctggccgtcg gtgcagtcca gccggccttc ggggcgcagg tggatgacgt
     2101 cgtactcgac gtcgctgcgc tggatcggca cgcccgcccc cgcacggggg cgttcgtcat
     2161 tctgcccgtg ggcctggcgc ggcacgcgaa aggcccagcc gcgcgactgc gaggcgtcgg
     2221 acgacagcat cagacagtcg tgctgggcca gctcgcgcgg gtggcgtggc cggccgcgcc
     2281 gcgccaggta ggcgggcgcg gccacgcaca ggcgctggtt gtcggccagg cggatgctga
     2341 tgagcgacga gtccggcaga tcgcccacgc gcaccgcgca gtcgtagcct tcggccgcca
     2401 gatccaccac ccggtccgac aggttgagcg aaatgcgcag gcgcgggtgc agggcgtgga
     2461 actcgggcac cagcggcgcc acatggcggc gcccgaagcc ggccggtgcc gtcacgcgca
     2521 ggtgaccgct ggcctgcacg ccgccggccg acacgctggc ctcggccccc gccacctcgg
     2581 ccaggatgcg ctggcagtcg tccagaaagg ccgtgccttc atgcgtcagc gcgatgcggc
     2641 gcgtggtgcg caccagcagc ttgacgccca ggtgctcctc cagcgcgtcc aggcgccggc
     2701 tgaccaccgc cggggccacg ccctcggcgc gcgcggcggc gctcaggctg ccgcgcgtgg
     2761 ccaccgaggc gaaggtctcg aaggccttga gcttgtccat gcgcggcaat tatgcgcagc
     2821 cgcgcccatc ggttgcggcc ggcgcgccac aggccgctgc gccatgcggg cgtgacgtgc
     2881 gatcatgcgc gctcacatct gcctgcaccc atgcgagccg aatccgcccc gccgatcgac
     2941 aggcgccaca tcgatgtgcg cacgatgagc ctggcgcagc tgcagaagct ggccgagcgc
     3001 ggcagccggc gcgcccgcgc cgaactggag gcgcgcatgc gggcgcccgg ccccgtcctg
     3061 ctgccggcgt ccgtgccggc cgaccgcacg gcaccatccg cgccgagccg gcacccgccg
     3121 acgctgaccg cgcgcgccga tccgccgccg caggccaccg ccgccggagc ggcagcgccc
     3181 gcgcccggcg cggtgccggc tgggcagggg gtgccggtgt cggccgacgc gctggcgcag
     3241 caactgcacc tgatcgcccg gcaggacgag gcgcacgccc gcgccgccgg gccgccgcgc
     3301 ctggtcggcc tggtgctgac ggcctggggc gtgctgctgc tgctgggagg gctgctgatg
     3361 ctcgggcgcg gtggcgcggc gctgtactac ctgttctgcg gcctgggcgc ggccgccgtc
     3421 ggcgcgctgc tgatgcggcg cagccgctgg gccctggccc tgcatggcgc cctgctgccg
     3481 ctggcgctgg gctgggcctg ggccggtgcg gggcgccaca gcctgcccct gagcgtggtg
     3541 caggcggggc ccttgctgat cccggcgctg tggatggcgc tgcgccaggt gcgcgaggcg
     3601 ctggaatgat atgcattgaa cctgggttaa aagtgcctcc ggcgcttgat gggcaagcgt
     3661 cagcagctat cgtattcata gtgaactgag ggttccgatc cgacccgggg ggcgccgtgc
     3721 gggcgttggc tggccgatct ttgcttcaga cgcaaaggtt ttttggtttt tcacctgttt
     3781 gatgcccaaa acaaatcgaa taaagtgccc acaccgggcg ccaggcgcct gttttttctt
     3841 tgtttctaca aggccacccc atgaccgcac gcacccgcat tcacgacctg cacgttgccg
     3901 acacgctgta cgagttcatc aaccgtgagg ttctgcccga cgtcggcgtg gacgcggcgc
     3961 gcttctggca gggtttttcg gccatcgtga ccgagctggc acccaagaac atcgccctgc
     4021 tggccgagcg cgaccgcctg cagaaggagc tcgacacctg gcaccaggcc aaccccggcc
     4081 cgatcaagga catgcgcgcc taccgcgcct ttctggagcg catcggctac ctggtgccgc
     4141 agccggccaa ggtgaaggtg accaccaagc gggtcgatgc cgagctggcc gtgcaggccg
     4201 gcccccagct ggtggtgccg atcctcaacg cccgctatgc gctcaacgcc gccaacgcgc
     4261 gctggggcag cctgtacgac gcgctgtacg gcaccgacgt gatccccgag accaagggcc
     4321 tggaaaaggg cgccaagtac aacccccgcc gcggcgccaa ggtgatcgag tgggcgcgcc
     4381 acgtgctgga ccgcacggcg ccgctcaaga agggctcgca cgtcgattcg gtcgcctacc
     4441 tggtgcgcga cggcgagctg gtggtcaagc tcaaggacgg caagagcagc aagctggccg
     4501 acccggcgca gttcatcggc taccagggcg agcccaaggc gccgtcgtcc atcctgctgc
     4561 tgcaccacgg cctgcacctg gacatccgca tcgacccggc gtcgcccatc ggccgcaacg
     4621 acccggccgg cgtggccgac ctggtggtgg aggcggcggt ctccaccatc ctggatctgg
     4681 aggactccat cgccgccgtc gatgccgagg acaaggtgct gggctaccgc aactggctgg
     4741 gcatcctgcg cggcacgctg accgagtcgt tcgacaaggg cggcaagacc ctgacgcgcg
     4801 gcctcaaccc cgaccgcctc tacaccgccg ccagcggcaa gggccaggtg cggctgcacg
     4861 ggcgctcgct gatgttcgtg cgcaacgtcg gccacctgat gaccaacccg gccatcctgt
     4921 atcgggcggc cgacggcagc cagcgcgaga tccccgaagg catcatggac gccgtggtca
     4981 ccaccaccat cgcgctgcac gacctgcagc gcaagggcgc gaacggcatc cgcaactcgc
     5041 gcaccggcag cgtctacatc gtcaagccca agatgcacgg cccggccgag gtcggcttcg
     5101 cggccgagct gttcggccgc gtcgagcagc tgctgggcct ggcggacagc accgtcaagc
     5161 tgggcatcat ggacgaggag cgccgcacca gcgtcaacct caaggcctgc attgccgccg
     5221 ccaagagccg cgtggccttc atcaacacgg gctttctgga ccgcaccggc gacgagatgc
     5281 acagctgcat gcgcgccggc ccggtctacc gcaagggcga catgaagacc agcgcctgga
     5341 tccaggcgta tgagcgcaac aacgtgctgg tcggtctggc ctgcggcctg cgtggcaagg
     5401 cgcagatcgg caagggcatg tgggccatgc ccgacctcat gaaggcgatg ctggagcaga
     5461 agatcgccca ccccagggcc ggcgccaaca ccgcctgggt gcccagcccc accggcgcca
     5521 ccctgcacgc catgcactac caccaggtgc tggtgagcga ggtgcagcag cgcctggagg
     5581 ccgagctggc ccaggccgac ccggcggcgc tgcgcgacga gctgttgacc ggcctgctga
     5641 ccatcccggt ggtgagcgaa gacaagtgga ccgccgccga gaagcagcag gagctggaca
     5701 acaacgccca gggcattctg ggctacgtgg tgcgctgggt cgatcagggg gtgggctgct
     5761 ccaaggtgcc cgacatccac aacgtcggcc tgatggaaga ccgcgccacg ctgcgcatca
     5821 gcagccagca catggccaac tggctgctgc acggcgtggt cagcgaaggt caggtgcgcg
     5881 ccacgctgca gcgcatggcg gcgctggtgg accagcagaa cggcggcgac ccgctgtacc
     5941 gccgcatgca cgaccgcttc gagcagtccg ccgccttcca ggccgcctgc gatctggtgt
     6001 tcaagggggt gcagcaaccc agcggctaca ccgagccact gctgcacgcc tggcgccaga
     6061 aggtcaaggc cggcgcttga aaaagcatag cttgcggcgc ccgaaaggcg ggcgccgcaa
     6121 gctgttcaga ccatgacaaa gggcgccgcg gcgcccttgg ttctgtgccg tcgcggtcag
     6181 caggtggttg cgcccgctca ggcgcgggcc aggccctgct tgtgctccca ggccgacttg
     6241 caggccacgc agtgctgcgt catgggttcg atctgcaggc gctcgaaggg aatctggcag
     6301 ccgcattcgt cgcagcggcc gtaatcgccg gcgtccatgg cttccagcgc gcggtcgatg
     6361 cgcgccagct cttcggcgtc cagcgcggtc aggcgcgtgt ccacctcgcg cgtctggttg
     6421 gccaggcggg cgttttcgtc ctgcgacacg ctgccggcgg tgttttcggc cggcgccagg
     6481 ttggcgcggt tctgctgcat ctgcgcgtac agctcctggc ggcgggccag cagccgctgt
     6541 ttcagctgct cgagttgggc gggggtcagg gtttggctca tgctccatct ttcctgcaaa
     6601 gtcgcggatc accatgccgc gcggcggcgc ggcgcttcat cccgcagaat accacccaca
     6661 tgccgctgtc cgcgccaccg atcgacccca cgcgctgccc cctgtgcggg cagccgaacc
     6721 ggtgcgcgca gcaggcgcgt cctgccggtg cgccggcggc gccgcactgc tggtgcatgg
     6781 agcggcgcgt ggccccggac gcgctggcgc gcgtgccggc gcaggcgcgc gggcgcgcct
     6841 gtctgtgccc ggcctgcgcc accccgaggg cgtgaacgcg cagccgccga tgcacgatcc
     6901 gcccccgtcc agttcggccg aggcgctgcg cgcgcgccat ggcgcgcgct ggcgctggct
     6961 gctgctgctg acggtgatgg tgggcaccat ggcctcgatc atgtcctcca ccgtggtcaa
     7021 cgtggcgatt cccgggctgt cgcagcagtt caccctgggg caggcgcgcg cacagtgggt
     7081 cagcagcggc ttcatggtgg cgatgacggt gtcgatgctg accacgccct ggctgctggc
     7141 gcgcttcggc taccgcgcca cctacaacgg ctgcatgctg ctgctgctgg ccggcggcgt
     7201 gctgggcggc gtggcgcaga gctacccgct ggtgctggcc gcgcgcgtgg ccgaagggct
     7261 ggcggcgggg gtggtgcagc cgatcccggc ggtgatcatc atgcgcgcct tcgagcccaa
     7321 cgagcagggc cgcgccagcg gcctgttcgg catgggggtg gtgctggcgc cggcgctggg
     7381 gccgtcgatc ggcggcgtgc tggtggactg gttcggctgg cgctcgatct tcttcatggt
     7441 ggcgccgttc tgcctgctgg cgctgtggct ggcgcgccgc tacgtgccga ccacggcacc
     7501 cggcggcgcc gccacccggc gcgacgagac gctcgacggc gtcggcctgc tgctgggcag
     7561 cgccggcacg ctgtgcctgc tcaacgggct ggtggcactg cgcgccgggc cggcggtcga
     7621 agcggcggcg ctgctggcgc tggcggcgat ctgcctggcc ggcttcatcg gctggcagcg
     7681 ccgctgcgtg cgcctcgggc gcgcgccgct gatgaacctg gcgctgttcg cctggcgacc
     7741 gtttgccatg ggggtgctgg tggcgctgat ctacggcacg gcgctgttcg gctccaccta
     7801 cctgctgccg gtctacatgc agatggggct ggggttgtcg gcctccagcg tgggcgccat
     7861 cctgctgccg gccggcctgg tgctggcggc caccatcccg ctggtggcgc gctgggccga
     7921 ccggctgccg gccagcggcc tggtgaacac cgggctggcg ctgctggcac tgtcgttcgc
     7981 ggccatggtg acggtggggc tggcgcccgg gctgtggcac ctgactgcgc tgggcatcgt
     8041 cgggcgcatc gggctgggct tcatcctgcc ctcactcaac ctgggcgcca tgcgcgggct
     8101 ggcgctgccg ctgatcccgc agggctccag cgtcatcaat ttcgcgcgca ccctgggcgg
     8161 ggcggccggc atcagcctgt gcggcatcgc actggaatgg cggctggcgg cgcatggcac
     8221 ctcgctgacc gaaggtgcgc ccagcgcggc gcggttggcg gcgttcgacg aagtgttcct
     8281 gatgctggcc gcgctgtgcg gcatggccat gctggcggcg cgccacctga gcacgccggc
     8341 cgcgggtgcc gaccgataaa ctgcccgcca tgtgccagct gctgggaatg aactgcaaca
     8401 cgccgaccga cgtgagcttc agcttttccg gcttcgcgca gc
//
LOCUS       DAOOOG010000113         8437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_9865_length_8437_cov_27.754832, whole genome shotgun sequence.
ACCESSION   DAOOOG010000113 DAOOOG010000000
VERSION     DAOOOG010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9865_length_8437_cov_27.754832"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PK242_09050"
     CDS             <1..601
                     /locus_tag="PK242_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOZ94206.1"
                     /translation="INPFGLHYSEVTASNLVKVNLAGDILSESRYPINPAGYVLHSCI
                     HDGVPGAHCVMHTHTTAGVAVASLASGLSQSNFYSAQLHDMVAYHDFEGITIRAEEGP
                     RVVASIGQRKAVILRNHGLLSWGETLPLAFAVLWTLQRACEIQLATQSMGAPLPVPEG
                     IAAACTRDSFQFDPARGAGQDVFDALVRQVDRVDGSWRQ"
     gene            679..1011
                     /locus_tag="PK242_09055"
     CDS             679..1011
                     /locus_tag="PK242_09055"
                     /inference="COORDINATES: protein motif:HMM:NF015807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOZ94207.1"
                     /translation="MTTLTIKDVPEPLAQQLRQRAARNHRSLQGELMSIIEQAVQQGD
                     QPGAGLIGTRTLGGGTIVGMSAQGPIVRRGYKTIEQIYDELRAKYPEPIENQCDSVQL
                     IREDRDSR"
     gene            1008..1436
                     /locus_tag="PK242_09060"
     CDS             1008..1436
                     /locus_tag="PK242_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOZ94208.1"
                     /translation="MSPQLHVAEPPAPYLARPALVIDCSVLAGLVFREHWYEAARDQV
                     HGSTPHAPHLLIYEISNVALKKHRRQEAHALPGLAHALTLDIELHEVDMAQTFALAQR
                     YQLSACDASYLWLAGALRCPLATFDETLAQAAQQHLGALG"
     gene            1601..2599
                     /locus_tag="PK242_09065"
     CDS             1601..2599
                     /locus_tag="PK242_09065"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOZ94209.1"
                     /translation="MFHKICIYGAGAIGGWLGARLAAQGAAVGAVARGATLAALRAHG
                     LRLREAGDDGAVTETAHAVQASEQPAELGVQDLVIVAVKAPAMGQVAAHIAPLLGPDT
                     VVLTAMNGVPWWFLQGFGGALAGTRLESVDAGGSIAAAIEARRVIGAVVHASCSVDAP
                     GVIRRHFGNGLIVGEPGGGDTPRLAALAALLRQARFEVTVSPQIQKDVWYKLWGNMTI
                     NPISALTGASADRILDDDLVRGFVSAVMLEAREIGARIGVPIEQTPDDRHQVTRKLGA
                     FKPSMLQDVEAGRPVEIDALVGAVRELGQLTGVATPCTDALLGLARLHARVRGLYV"
     gene            2592..3497
                     /locus_tag="PK242_09070"
     CDS             2592..3497
                     /locus_tag="PK242_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOZ94210.1"
                     /translation="MSERLSARAAAGFATLLLIALLMAANHVSARLAFQHGVDVPTAV
                     TVRSLGTALVVGLLLHWQQVPLALNARQRKALPLIGALIAVQSLCLYSAVARLPVALA
                     LLVFNSFPLMTALWARLLYQHRPQRAVLVAMPVLLLGLLLALDVLGAASGLGAAGQWR
                     QIGVGVAFALGAAATFGLALVLTQHEAGALDGRLRTLATMTIVGLLAGAVALARGGLA
                     LPQATPGWWGLALLTLLYGTGITLLFTVLPRLGVVGHSAIMNVEPVFALILAWLVLGQ
                     AVAPVQVAGALVVVGAVVWLGLWRR"
     gene            3541..4335
                     /locus_tag="PK242_09075"
     CDS             3541..4335
                     /locus_tag="PK242_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HOZ94211.1"
                     /translation="MTEALTPPPEPAEPAADPRADTLEALEGALDALREHHPQRVPQW
                     EYCEGVLTALLCMRRTVPPTEWLPLLFDGDDVFASEGERTQFMMHWLAREAQLRAQLQ
                     APVEALDDEQALEPGVIDWRGLLASLPEDERAAATAEGTPPPIAQAWAGGFIDAVDYW
                     AEDWAPPRDKEIAAQMDDALDCIATLLEEDREAPKFNLFDEHGAPSVSQARFEAYGEA
                     IWAVYDLFDIARSLGPRIAPARSDKVGRNDPCPCGSGKKFKKCCGA"
     gene            complement(4506..5429)
                     /gene="rsmI"
                     /locus_tag="PK242_09080"
     CDS             complement(4506..5429)
                     /gene="rsmI"
                     /locus_tag="PK242_09080"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOZ94212.1"
                     /translation="MSDSAPVGKADLHAALAAAHRAAGAQHYPAGSLYVVATPIGNLA
                     DITLRALHVLALADAIACEDTRHTGQLLRAYGIDGKPLLALHEHNEREAAATVIARLR
                     AGERVAYVSDAGTPAVSDPGARLVAACAAEGLRVVPLPGASSVTAALSAAGLAGDGQF
                     LFRGFLPARASERQAAVQALAAEPRAVVLLEAPHRIAALADALAALDQRAITVARELT
                     KQFEQLATMACAHLPRWLASEPQRQRGEFALVLHPAPDTPGPGDGERVLRLLLSELPL
                     KTAVKLAAELSGAPRNALYQQALRWQGEPGR"
     gene            5448..5837
                     /locus_tag="PK242_09085"
     CDS             5448..5837
                     /locus_tag="PK242_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOZ94213.1"
                     /translation="MRGWGGRQQPGASTRVRGAAAEDMALAHLLRAGLRLIERNYRTP
                     GRGGGEIDLVMRAPDGTLVFVEVRARASAAHGGAAASVGAVKQRRIVLAAHHYLLRFG
                     APPRCRFDVVAIEGTHIDWLPAAFDAG"
     gene            5936..6535
                     /locus_tag="PK242_09090"
     CDS             5936..6535
                     /locus_tag="PK242_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOZ94214.1"
                     /translation="MPEPRTSQQFIDSADLKYQWAQTLARPIDEATQALLACITGGGK
                     LMVCGNGASGALAQYLVALLVGGLERARPELPALALGADAATLTAVARRGEYADVFAA
                     QVRALGNPGDALLLLSTNGNTVNLVRAAQAAHERDMCVIALSGRGGGELGRMLRDTDV
                     HVCVPHERTPRIHEAHQLVLHCLCDGLDLQLLGHEESSE"
     gene            6532..7269
                     /locus_tag="PK242_09095"
     CDS             6532..7269
                     /locus_tag="PK242_09095"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOZ94215.1"
                     /translation="MKSFCMRGVGMALAAALLSGCAAAVVGGAVGTGVLLSSADRRSG
                     DTQQADQTIEAAAREAVAQTLAGRGHVNVASYYRKTLITGEVGSAQDRLQVEAQVRAI
                     PGVQGVVNELAVMPESSAFQRSNDAFITSKVRSRLVGTNGVPSGALKVLTERGTTYLM
                     GRLTATELALATDAVRQTEGVQRVVRVVDLIVEPASGALAPPVQGATPPAPATEAANG
                     VQTHPVTQPAIVHAPAPIQVQTLPPVK"
     gene            complement(7305..8222)
                     /locus_tag="PK242_09100"
     CDS             complement(7305..8222)
                     /locus_tag="PK242_09100"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ94216.1"
                     /translation="MNTTIHARSYDPTPARRVAFLGLGVMGYPMAGHLALAGHEVTVY
                     NRNATKSEAFCAQLAGAGTVKRAQTPATAAAGVEIVLACVGNDDDLRQVVLGEHGAFA
                     GMAPGAVFVDHTTASATVARELDALARSRGLHFIDAPVSGGQAGAQNGQLTVMCGGDP
                     AAFERVRPVAMAFSRAFTLMGPSGAGQLTKMVNQICIAGLVQGLSEAIAFGQRAGLDM
                     AQVIEVIGKGAAQSWQLDHRGPTMVQDRFDFGFAVDWMRKDLGLVLDEAKRNGARLPV
                     TALVDQFYADVQQLGGGRWDTSSLIRRLK"
BASE COUNT         1199 a         2918 c         3096 g         1224 t
ORIGIN      
        1 catcaacccc ttcggcctgc actacagcga ggtcacggcc agcaacctgg tcaaggtcaa
       61 cctggccggc gacatcctga gcgagtcgcg ctaccccatc aaccccgccg gctacgtgct
      121 gcacagctgc atccacgacg gcgtgcccgg cgcgcactgc gtgatgcaca cccacaccac
      181 ggccggggtg gcggtggctt cgctggccag cgggctgtcg caaagcaact tctacagcgc
      241 gcaactgcac gacatggtgg cctaccacga cttcgagggc atcaccatcc gcgccgagga
      301 agggccgcgg gtggtggcca gcatcggcca acgtaaagcc gtgatcctgc gcaaccacgg
      361 cctgctgtcc tggggcgaga cgctgccgct ggcctttgcc gtgctgtgga cgctgcagcg
      421 cgcctgcgag atccagctgg ccacgcaaag catgggcgcg ccgctgccgg tgcccgaagg
      481 catcgccgcc gcctgcacgc gcgactcgtt tcagttcgac cccgcgcgcg gcgccgggca
      541 ggacgtgttc gacgccctgg tgcggcaggt cgatcgcgtg gacggcagct ggcgccagta
      601 gggcgctggg cgccgtttga cagtgcctgc cggcggcatg caaaatgcat ccattgtgga
      661 tccattctgg atcatgccat gaccaccctg accatcaagg acgtgcccga gcccctggcc
      721 cagcagctgc gccagcgcgc cgcgcgcaac caccgctcgc tgcagggcga gctgatgagc
      781 atcatcgagc aggccgtgca gcagggcgat cagcccggcg ccgggctgat cggcacgcgc
      841 accctgggcg gcggcaccat cgtcggcatg agcgcgcagg ggcccatcgt gcggcgcggc
      901 tacaagacga tcgagcagat ttatgacgag ttgcgtgcca agtatcccga gccgatcgaa
      961 aatcagtgcg acagcgtgca gctgatccgc gaagaccgcg acagccggtg agcccgcagc
     1021 ttcatgtggc ggagccgccc gcgccctatc tggcgcgccc ggccctggtg atcgattgct
     1081 cggtgctggc cgggctggtg tttcgcgagc actggtacga ggccgcccgc gaccaggtgc
     1141 atggcagcac accgcatgcg ccccatctgc tgatctacga gatcagcaac gtcgcgctca
     1201 agaagcaccg acgtcaggaa gcgcatgccc tgcctgggct ggcgcatgcg ctgacactgg
     1261 acatcgaact gcacgaagtg gatatggcgc aaacctttgc cctggcccag cgctaccagc
     1321 tcagcgcctg cgacgccagc tacctgtggc tggccggcgc gctgcgctgc ccgctggcca
     1381 ccttcgacga aacgctggcc caggccgcgc agcagcatct gggggcgttg ggctgacttg
     1441 agggtgggtt gaagaatgac gaatgacaaa tgacctcgct gcgcttcgct gattgaagaa
     1501 gctggggtgt atgcgacggc caggtggcct gatgcatgcc cattatttac tattgatttg
     1561 atatctactg gcgcttgctg gacaagcgct ggaggccaaa atgtttcata aaatctgcat
     1621 ctacggtgcc ggcgccatcg gcggctggct gggcgcgcgg ctggcggcgc agggcgcggc
     1681 cgtcggcgcc gtggcgcgcg gcgccacgct ggcggcgctg cgcgcgcatg ggctgcgctt
     1741 gcgcgaggcg ggggacgatg gggcagtgac tgaaaccgcg cacgccgtgc aggccagcga
     1801 gcaaccggcc gagctgggcg tgcaggatct ggtgatcgtc gccgtcaagg cgccggccat
     1861 ggggcaggtg gcggcgcaca tcgcgccact gctggggccc gacaccgtgg tgctgacggc
     1921 catgaacggc gtgccctggt ggtttctgca gggctttggc ggcgcgctgg cgggcacgcg
     1981 gctggagtcg gtcgatgcgg gtggcagcat tgccgccgcc atcgaggcgc gccgcgtgat
     2041 cggtgccgtg gtgcacgcca gctgctcggt cgatgcgccg ggggtgatcc gccgccactt
     2101 cggcaacggt ctgatcgtgg gcgagcccgg gggcggcgac acgccgcgcc tcgccgccct
     2161 ggccgcgctg ctgcggcagg cgcggttcga ggtcaccgtc tcgccgcaga tccagaagga
     2221 cgtctggtac aagctgtggg gcaacatgac catcaacccc atcagcgccc ttaccggcgc
     2281 cagcgccgac cgcatcctgg acgatgatct ggtgcgcggc ttcgtcagcg ccgtgatgct
     2341 ggaggcgcgc gagatcggcg cgcgcatcgg cgtgccgatc gagcagacgc ccgacgaccg
     2401 ccaccaggtc acgcgcaagc tgggcgcctt caagccctcg atgctgcagg acgtggaggc
     2461 cggccgcccg gtcgagatcg acgccctggt gggcgcggtg cgcgagctgg ggcagctgac
     2521 cggtgtggcc acgccctgta ccgacgccct gctgggcctg gcgcggctgc acgcacgggt
     2581 gcgcggcctg tatgtctgag cgcctgtcgg cgcgtgccgc ggccggtttc gccaccctgt
     2641 tgctgatcgc cctgctgatg gcggccaacc acgtgtcggc gcggctggcc ttccagcacg
     2701 gcgtggacgt gcccaccgcc gtcaccgtgc gcagcctggg cacggcgctg gtggtgggcc
     2761 tgctgctgca ctggcagcag gtgccgctgg cgctcaatgc ccgccagcgc aaggcgctgc
     2821 cgctgatcgg cgcgctaatc gcggtgcaga gcctgtgcct gtattcggcc gtggcgcggc
     2881 tgccggtggc gctggcgctg ctggtgttca acagctttcc gctcatgacc gcgctgtggg
     2941 cgcgcctgct gtatcaacac cgtccgcagc gtgcggtgct ggtggccatg ccggtgctgc
     3001 tgctgggcct gctgctggcg ctggacgtgc tgggcgccgc ttccggcctg ggcgcggccg
     3061 ggcagtggcg gcagatcggc gtcggcgtgg cattcgccct gggggcggcg gccaccttcg
     3121 gcctggccct ggtgctgacg cagcacgagg ccggcgcgct ggacgggcgc ctgcgcaccc
     3181 tggctaccat gaccatcgtc ggcctgctgg ccggcgccgt ggcgctggcg cgcggcggcc
     3241 tggcgctgcc gcaagccacg cccggctggt gggggctggc gctgctgacc ctgctgtacg
     3301 gcaccggcat caccctgctt ttcaccgtgc tgccgcgcct gggggtggtc ggccattcgg
     3361 ccatcatgaa cgtggagccg gtgttcgccc tgattctggc ctggctggtg ctggggcagg
     3421 cggtggcgcc ggtgcaggtg gccggcgcgc tggtggtggt gggcgcggtg gtctggctgg
     3481 ggctgtggcg gcgttgacgc cgcgtgtcgt taccatcggc gcagccaaag gatttgcgcc
     3541 atgaccgaag cccttacccc gccacccgaa cccgccgagc ccgccgccga cccgcgcgcc
     3601 gatacgctgg aggcgctgga gggcgccctg gacgcgctgc gcgagcacca cccccagcgc
     3661 gtgccgcagt gggaatactg cgagggcgtg ctgacggcgc tgctgtgcat gcgccgcacc
     3721 gtgccgccga ccgaatggct gcccctgctg ttcgatggcg acgacgtgtt tgccagcgaa
     3781 ggcgagcgca cccaattcat gatgcactgg ctggcgcgcg aggcgcagct gcgcgctcag
     3841 ctgcaggcgc cggtcgaggc gctggacgac gagcaggcgc tggagccggg cgtgatcgac
     3901 tggcgcggcc tgctggccag cctgcccgag gacgagcgcg ccgcggccac ggccgagggc
     3961 acgccgccgc ccatcgcgca ggcctgggcc ggcggcttca tcgacgcggt ggactactgg
     4021 gccgaggact gggcgccgcc gcgcgacaag gaaatcgccg cccagatgga cgacgcgctg
     4081 gactgcatcg ccacgttgct ggaggaagac cgagaggcgc ccaagttcaa cctgttcgac
     4141 gagcacggcg cgcccagcgt cagccaagcg cgcttcgaag cctatggcga agcgatctgg
     4201 gcggtgtacg acctgttcga catcgcgcga agcctgggcc cgcgcatcgc cccggcccgc
     4261 agcgacaagg tgggccgcaa cgacccctgc ccctgcggca gcggcaagaa gttcaaaaag
     4321 tgctgcggcg cctgatgcta tctgaacaat agctgttggc gcttgctgga taagcgcaat
     4381 gaccgcgacg atgccacact ggtgcgccac cccaggccac gccggcggtt cgggagctgc
     4441 ggcgccagcc atgccaggtt cgccgatcac ggcaacggca gcccggccac tggccgttct
     4501 ttggctcagc ggcccggttc gccctgccag cgcagggcct gctgatacag cgcgttgcgc
     4561 ggtgccccgg acagctcggc cgccagcttg acggcggtct tcagcggcag ttcggagagc
     4621 agcagccgca gcacgcgttc gccatcgcca ggccctgggg tgtctggggc agggtgcagc
     4681 accagcgcga actcgccgcg ctggcgctgc ggctcggacg ccagccagcg tggcaggtgg
     4741 gcgcaggcca tggtggcgag ctgctcgaac tgcttggtca actcgcgtgc cacggtgatg
     4801 gcgcgctggt ccagcgcggc cagcgcgtcg gccagcgcgg cgatgcggtg cggcgcctcc
     4861 agcagcacca cggcacgcgg ctcggcggcc agcgcctgca ccgcggcctg gcgctcgctc
     4921 gctctggcgg gcagaaatcc gcggaacagg aactggccgt cgcccgccag accggcggcg
     4981 ctgagggccg cggtcacgct gctggcgccc ggcagcggca ccacccgcag accctcggcg
     5041 gcgcaggctg ccaccaggcg cgcgcccggg tcgctgaccg ccggcgtgcc ggcgtcgctg
     5101 acgtaggcca cgcgctcgcc ggcccgcagg cgcgcgatca cggtggcggc ggcctcgcgc
     5161 tcgttgtgtt catgcagcgc cagcagcggc ttgccgtcga tgccgtaggc gcgcagcagc
     5221 tggccggtgt ggcgcgtgtc ttcgcaggcg atggcgtcgg ccagggccag cacatgcagc
     5281 gcgcgcaggg tgatgtcggc caggttgccg atcggtgtgg ccaccacgta caggctgccc
     5341 gccggataat gctgcgcgcc ggcggcccgg tgcgccgccg ccagggcggc atgaaggtcg
     5401 gctttgccga cgggggcgga atcagacatg gacggggcct tgacgtgatg cgtgggtggg
     5461 gcgggcgcca gcagccggga gccagcacac gcgtgcgcgg agccgccgcc gaagacatgg
     5521 cgctggcgca cctgctgcgc gccggcttgc gcctgatcga gcgcaattat cgaaccccag
     5581 ggcgcggggg cggcgaaatc gatctggtga tgcgtgcacc cgacggcacg ctggtgttcg
     5641 tcgaggtgcg cgcgcgcgcc agcgccgcgc atggcggggc ggcggccagc gtgggcgcgg
     5701 tcaagcagcg gcgcatcgtg ctggcggcgc accactacct gctgcgcttc ggcgcgccgc
     5761 cgcgctgccg attcgacgtg gtggcgatcg aggggacgca catcgactgg ctgccggcgg
     5821 ccttcgatgc cggctgaggc gggcgccggc cggcccgggt cgccgccctc gccgcaacac
     5881 ctgcttgcta ccaaatttgc accgtggcgg ggcggcgcgg ttatgatcga cgccgatgcc
     5941 cgaaccccgc acgtcacagc agttcatcga cagcgccgac ctgaagtacc agtgggcgca
     6001 gaccctggcg cggcccatcg acgaggccac gcaggcgctg ctggcctgca tcaccggcgg
     6061 cggcaagctg atggtgtgtg gcaatggcgc gtccggcgcg ctggcgcagt acctggtggc
     6121 cttgctggtg ggcggtctgg agcgcgcccg tcccgaactg ccggcactgg cgctgggcgc
     6181 cgatgccgcc accctgaccg cggtggcgcg gcgcggtgaa tacgccgatg tcttcgcggc
     6241 ccaggtgcgc gcgctgggca acccgggcga cgcgctgctg ctgctgtcga ccaacggcaa
     6301 cacggtcaac ctggtgcgcg ccgcgcaggc cgcgcacgag cgcgacatgt gcgtgatcgc
     6361 gctcagcggg cgcgggggcg gcgagctggg tcggatgctg cgcgacaccg acgttcatgt
     6421 gtgcgtgccg cacgagcgca cgccgcgcat ccatgaggcg caccagctgg tgctgcattg
     6481 cctgtgtgat ggcctggatc tgcaactgct gggtcacgag gagagttccg aatgaaatcg
     6541 ttctgcatgc gcggggtggg catggcgctg gccgccgccc tgctgagcgg ctgcgcggcg
     6601 gcggtggtgg gtggtgcggt gggcaccggt gtcctgctga gttcggccga ccgccgcagt
     6661 ggcgacaccc agcaggccga ccagacgatc gaggcggcgg cgcgcgaggc cgtggcccag
     6721 acgctggcgg ggcgcggcca tgtcaacgtc gccagctact accgcaagac cctgatcacc
     6781 ggcgaagtcg gtagcgcgca ggatcgactg caggtggagg cgcaggtgcg cgccatcccg
     6841 ggcgtgcagg gcgtggtcaa tgagctggcg gtgatgcccg aatccagcgc cttccagcgc
     6901 tccaacgacg ccttcatcac cagcaaggtg cgcagccgcc tggtgggcac caatggcgtg
     6961 ccctcgggcg ccctcaaggt gctgaccgag cgcggcacga cctacctgat gggccgcctg
     7021 acggccaccg aactggcgct ggccaccgac gccgtgcgcc agaccgaagg cgtgcagcgc
     7081 gtggtgcggg tggtcgatct gatcgtcgag ccggcatccg gcgcgctggc gccgccggtc
     7141 cagggtgcca ccccgccggc gccggccacg gaggcggcca acggcgtgca gacccacccg
     7201 gtcacgcagc cggccatcgt ccacgcgccg gcaccgatcc aggtgcagac gctgcccccg
     7261 gtcaagtaga cggtcggggc ctggggcggt cccaggcgcg ggcgttactt caggcgccgg
     7321 atcaggctgg aggtgtccca gcggccgccg cccagctgtt gcacgtcggc atagaactga
     7381 tcgaccagcg ccgtcaccgg caggcgggcg ccgttgcgct tggcttcgtc cagcaccagc
     7441 cccaggtcct tgcgcatcca gtccaccgca aagccgaaat caaagcggtc ctgcaccatg
     7501 gtcggaccac ggtggtcgag ctgccagctc tgcgccgcgc ccttgccgat gacctcgatc
     7561 acctgcgcca tgtccaggcc ggcgcgctgg ccgaaggcga tcgcctccga caggccctgc
     7621 accagccccg caatgcagat ctgattgacc atcttggtca gctgcccggc gccgctcggc
     7681 cccatcaggg tgaaggcgcg cgagaacgcc atggccaccg gccgcacgcg ctcgaacgcc
     7741 gccggatcgc cgccgcacat cacggtcagc tggccgttct gcgccccggc ctggccaccc
     7801 gagaccggcg cgtcgatgaa gtgcaggccg cggctgcgcg ccagcgcgtc cagctcgcgc
     7861 gccacggtgg ccgaggcggt ggtgtgatcg acgaacaccg cccccggcgc catgccggca
     7921 aaggcgccat gctcgcccag caccacctga cgcaggtcgt cgtcgttgcc cacgcaggcc
     7981 agcacgatct ccacaccggc cgccgcggtc gccggagtct gcgcacgttt tacggtgccg
     8041 gcgcccgcca gttgtgcgca gaacgcttct gattttgtag cattccggtt gtaaacggtg
     8101 acctcgtggc cggccagtgc caggtggcca gccatgggat agcccatcac gcccaggccg
     8161 agaaaggcca cacggcgggc cggggtgggg tcgtagctgc gggcgtggat ggtggtgttc
     8221 atgcggcaaa ttttaggagc ctgtcgcacc tggcgcgcca aaagccagca tcggcaccgg
     8281 cgagacccgc gtggccaggg cttgcagccc catggacgag ctgtggggcc agccgccggt
     8341 gcgggcaggt gggcagcgtg ctcaccctgc agatgaaggc cgtcgaagcc gcgcgacgct
     8401 gcggggatgc ggcggtggcg aagcgtgccc gcggccc
//
LOCUS       DAOOOG010000114         8434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_9871_length_8434_cov_22.073159, whole genome shotgun sequence.
ACCESSION   DAOOOG010000114 DAOOOG010000000
VERSION     DAOOOG010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9871_length_8434_cov_22.073159"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..623
                     /locus_tag="PK242_09105"
     CDS             408..623
                     /locus_tag="PK242_09105"
                     /inference="COORDINATES: protein motif:HMM:NF017691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HOZ94217.1"
                     /translation="MSAPAATNSYRCDGRTHCSQMSSCEEATFFLKHCPGVKMDGDGN
                     GIPCERQWCSADKEQEHGQERWRGRRR"
     gene            951..1178
                     /locus_tag="PK242_09110"
     CDS             951..1178
                     /locus_tag="PK242_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94218.1"
                     /translation="MRRFWTGLLWALIGYVAGAFGGGLLVSLLSSNSHDRSLEAAMTG
                     AFVLGPLAAVVAFAVGYVRAGRRQTGRETEG"
     gene            1252..1899
                     /locus_tag="PK242_09115"
     CDS             1252..1899
                     /locus_tag="PK242_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94219.1"
                     /translation="MPAALLALLFALGAGAPAVAEQITVAPQGVYAEIDTRLAIDTLR
                     TLQTGSPAQITAAMDAIKAAPERYAPPVFYVLSHVLFDRGDRDDAAFWFYAGQLRARF
                     DANRCADVSARQAVAVLNQTYGKPINQYTFKNIPKLEALIPRVVDWDRKTPHRYDHRW
                     INLHGMGAMISGTGSPKQPPSLSLPADQWAPIAEKTRADYLEGFRQAMAQMKSRE"
     gene            2144..2548
                     /locus_tag="PK242_09120"
     CDS             2144..2548
                     /locus_tag="PK242_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HOZ94220.1"
                     /translation="MKQTYKGSCHCGAVRFEADIDFEAGTAKCNCSICTKARSWGAIV
                     RPDAFRLIHGESELSDYQFGPKVVHHYFCKHCGIRPFERGFHESVGGEFCSVQVSCLD
                     DLHIPDIFTGEVRHADGRNDNYLQSPSETRHL"
     gene            2752..3075
                     /locus_tag="PK242_09125"
     CDS             2752..3075
                     /locus_tag="PK242_09125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOZ94221.1"
                     /translation="MNHQDTAKPIRKLARITAKPGHGDQLRAALLELEAATRQELGCI
                     EFSFFQAISDPDAFVLLEHFADEHAFKLHMTLEHTRSFFGTNAERVAGIKAVDIPSFE
                     SPVTR"
     gene            complement(3137..5425)
                     /locus_tag="PK242_09130"
     CDS             complement(3137..5425)
                     /locus_tag="PK242_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOZ94222.1"
                     /translation="MSTVATLPLRAPALTGYKPFWAKRFGPAPYLPMSRAEMEALGWD
                     SCDVVIVTGDAYVDHPSFGMAVIGRVLEAQGFRVGIIAQPDWHSSEPFKALGRPNLFW
                     GVTAGNMDSLINRYTADRKIRSDDAYTPGDVAGKRPDRAAIVYSQRCREAYKDVPILL
                     GGIEGSLRRIAHYDYWSDSVRRSIVVDSKCDLLLYGNAERALVEIAHRLARREPVGQI
                     TDVRGTAFVRRTAAPGWREIDSTEVDRPGRVDALSSPYQTTAERAAAQGSHCDATKTE
                     AAGADPTSAAAQKGLEKPIALIRPDHARSVIRLPSYEQVRRDPVLYAHANRVLHLETN
                     PGNARALVQAHGEGRSARDVWINPPPIPLSTAEMDHIFDLPYARNPHPRYADERGRHD
                     GATRIPAWEMIRFSVNIMRGCFGGCTFCSITEHEGRIIQSRSEDSIVREVEAMRDKVQ
                     GFTGVVSDLGGPTANMYRLGCRDPAIEAVCRKPSCVYPGICPNLRTDHDPLIRVYRRV
                     RQLPGVKKVLIGSGLRYDLAVRSPAYVKELVQHHVGGYLKIAPEHTEQGPLSKMMKPG
                     IGDYERFRQLFDRFSAEAGKQQYLIPYFIAAHPGTSDQDMLQLALWLKRNGFRADQVQ
                     AFYPSPMATATTMYHTGVNPLKGVHRDGRGERVDTVKGERRRRLHKAFLRWHDPANWP
                     LLRQALKDMGRHDLIGSGKHHLIPAWQPAGSPAYQSPRRKNSSPAASPRKLQKQELPA
                     RTQPAPGRILTQHTGLPPRRST"
     gene            complement(5631..6155)
                     /locus_tag="PK242_09135"
     CDS             complement(5631..6155)
                     /locus_tag="PK242_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HOZ94223.1"
                     /translation="MFRLPQPARAHVASIPAATRLAVAAALLAVLAGCASPPPAPRPR
                     ATPSAPPRLPELAAFSAQLSGRQAVPPSASTGQGELVAVLNRNTGLLQWKLSFSGLSG
                     PVRAAHFHSPGMAGEVADPVLTLGRTVVSPLEGRAMLTPRQRADLLSGQWYVNLRTAA
                     YPEGELRGQLIERK"
     gene            complement(6269..6898)
                     /locus_tag="PK242_09140"
     CDS             complement(6269..6898)
                     /locus_tag="PK242_09140"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HOZ94224.1"
                     /translation="MSLPHWNRSHAVLAGLVAAGLLSACASVDLGRRYDPPPIRMPQP
                     LPPAQVPPAQPGGVQTQPVPPAQPVPQPLPPAGQSAPLPPTDASARLVTLSTRLDGAS
                     VMPPTRSAGSGQWDAIYDPDTRLLRWKTRWSGLSGAITGVQFHGPADAGQNAPAVLVW
                     PAPFGPSYEGRASLSAAQAMDLLAGRWYVSVFTAAYPLGELRGQVRVVP"
     gene            7060..7479
                     /locus_tag="PK242_09145"
     CDS             7060..7479
                     /locus_tag="PK242_09145"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOZ94225.1"
                     /translation="MPTLDLPALQQLIQPLLPGLLGVRLTEATAERVRAEMTVRPELC
                     TTGHVLHGGSHMALADTLGAIGTVLNLRPGQRTVTTDSSTKFIAGAAEGSVVTAEAVA
                     LHRGRSTQVWQTTIRNAEGRLCAVVTQTQLVLATPGE"
     gene            7499..8059
                     /locus_tag="PK242_09150"
     CDS             7499..8059
                     /locus_tag="PK242_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HOZ94226.1"
                     /translation="MSQPLSRHRLHPHPSTPAPAVRSLCVDVGRSDTAPGAWWLRFRL
                     QAALEALALPPPGASAGPADDLWRHTCFEAFVGRAQAAAYHEFNFSPAGDWAAYAFSG
                     PRQRASADQLPLAPRLQWQRADDTLTLTVELPRQALPAADHTDLLLGLSAVIEARDGQ
                     LSCWALAHPGPQPDFHHRAGWTARLP"
     gene            8070..>8434
                     /locus_tag="PK242_09155"
     CDS             8070..>8434
                     /locus_tag="PK242_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOZ94227.1"
                     /translation="MKFGLERFLEEPLLRAPLAGRRVALLAHPASVTRNLTHALDALA
                     ALPDVRLTAAFGPQHGLRGDKQDNMVESSDYTDPRLGIPVFSLYGEVRRPTPAMLETF
                     DVLLVDLQDLGCRIYTFITT"
BASE COUNT         1319 a         2830 c         2934 g         1351 t
ORIGIN      
        1 cgtgtggaga accagcgcat cgccgaggct tgggcagagt gggacaactt ggccggtctc
       61 aaacagttgg gtcacaccag tgcggcctaa cacatcattc caccggcctg cgcaaaaggc
      121 cgcgcaggcc ggtgtattca gacgttgaca caaatatcat gcgagttact tcatcgcgcc
      181 atctcttggt attcgcaacg ctttgcgcgg ccgccgggct gcatgccgcg ccactgaaca
      241 agtgcgtcat caacggtagc gtttcctatc agcaagcgcc atgccctgca aatcaggagc
      301 aaagaaagcg gcccacactg gacgaactga acgccgcaga aaaacagcga cgcgcagctt
      361 ccagcgcaga tagaaccaca ggctcgcttc cccccagaga ggtgcctatg tcggcgcctg
      421 cagcaacaaa cagctaccgc tgcgacgggc ggacgcattg ctcacagatg tcctcatgcg
      481 aagaggccac cttcttcctc aaacattgcc caggcgtcaa gatggacggg gatggcaacg
      541 gtattccttg cgagcggcaa tggtgcagtg ccgacaagga acaagagcac gggcaagaac
      601 ggtggcgagg gcgccgacgc tgataccagc tggtcacgcg ccagacattg cctgagaggg
      661 catatatgga gtccccatca acgtaaaggg aatgaccgat tgagggcact ggggcgtggc
      721 gcacctgcgt tcgcaaaatc cggcatctgt ggtgaactct tttctggtcc gtgccgacat
      781 ggaatccgcg ccaccgcgtc gccacacgtt gcaatcggca gacaagctgc ggcaagcgtt
      841 ttcggcgtag agcggtatgg gagcagcccg gttggtcatg atggtcgatc aaatccttcg
      901 caaactggac cggagcggtc aaaaatgcga tgaccagaaa gcacccaccc atgcgcagat
      961 tctggaccgg cctgctgtgg gccctcatcg gctacgtcgc gggggcattt ggcggcggcc
     1021 tgctggtgtc gctgctgtcg tccaacagcc atgaccgatc gcttgaggcg gcgatgaccg
     1081 gggcctttgt gctgggcccg ctggctgccg ttgtggcttt tgcagtcgga tatgtgcgcg
     1141 ccgggcgcag acagaccggg cgcgagaccg aaggctgaaa ccgggcgatc aaccccttcc
     1201 agattcaacc cacgcccagg caaccccatg cgccctcgcc cactcccccg catgcctgcg
     1261 gccctgctgg ccttgctgtt tgcactgggc gccggcgcgc ctgccgtggc cgagcagatc
     1321 acggtggcgc cgcagggcgt gtatgccgag atcgatacgc gcctggccat tgacaccctg
     1381 cgcacgctgc aaacgggcag cccggcgcag atcaccgccg cgatggacgc catcaaggcg
     1441 gcgcccgagc ggtatgcgcc gccggtgttc tatgtgctgt cccacgtgct gttcgatcgc
     1501 ggagaccggg acgacgcggc cttttggttt tatgccgggc agttgcgcgc ccggtttgac
     1561 gccaatcgct gcgcggatgt gtcggcgcgc caggcggtgg cggtgctcaa ccagacctac
     1621 ggaaagccga tcaaccagta caccttcaag aacattccca agctggaggc gctgatcccg
     1681 cgcgtggtcg actgggaccg caagacgccg catcgctacg accatcgctg gatcaacctg
     1741 cacggcatgg gcgcgatgat atcgggcacg gggtcgccaa agcagccccc aagccttagc
     1801 ctgcccgccg atcagtgggc accgattgcc gagaagacgc gggccgatta cctggaaggc
     1861 tttcgacagg ccatggcgca gatgaaaagc cgcgaatgag caatactggc acccatcgag
     1921 ttcgttcgat cggaaagcac ccaaccatgg gcaaattctg gcacccctgg gctccagacc
     1981 aaagcgccac aaaaccttgg gggaagggca tgttccgact ttccatgctg accgtcggat
     2041 tcgatggctt gttcgccgca gtcggccgcg tgcgactgct gaacctcagg cgttggtctg
     2101 cacggccaac gcggtcgctt tgatacacaa cgagaaagac gaaatgaagc aaacctacaa
     2161 gggcagctgc cactgcggcg cagtgcggtt cgaggcagac atcgactttg aagctggcac
     2221 cgccaagtgc aactgctcca tctgcaccaa agccagatcc tggggcgcca tcgtcagacc
     2281 agatgcattc cgcctcattc atggtgagtc cgagttgtcc gactaccagt tcggtcccaa
     2341 agtcgtgcat cactatttct gcaagcactg cggtattcgc ccgttcgagc gcgggttcca
     2401 cgaatcggtg ggtggagagt tctgctccgt acaggtgtcg tgcctcgacg atctccacat
     2461 tcctgacatt ttcactggcg aagtgcgcca tgcggacgga cgcaacgaca actaccttca
     2521 gtccccgtct gaaacccgtc acctttagtc cagctgccgg ccagcatttc gcgcacggtg
     2581 ctcaaggtgc tcacccgcac ttgtgcattc gaccgcccgt cagcctgacg tttgtccgtc
     2641 cgcgcctcgt cctgcgcttg aaaaagctgg aacgaccccc actctcctgc ctggcccatc
     2701 cacgcgctct tcaagctcgg ggagacgaac gaccgacatt gagaaggttc catgaaccac
     2761 caagacactg ccaaaccgat ccggaagctc gcacgcatca ccgccaagcc tggacacggc
     2821 gatcaactgc gcgcggcgtt gctcgaactt gaagcggcca cacgacagga gttgggatgc
     2881 atcgagttct ccttcttcca ggccatttcc gatccagacg cctttgtgct tctggagcac
     2941 tttgcagacg agcatgcgtt caagctgcac atgacgcttg agcacacgcg cagcttcttt
     3001 ggcaccaacg ccgaacgggt ggctggcatc aaggccgtcg acatcccgag tttcgagtcg
     3061 ccggtcaccc gatgaacccg tggcaaccca tgcccgtggg ttgcccctca gcgcccggcc
     3121 ggggccggct cggccgctat gtgctgcgcc gcggcggcag cccggtgtgc tgggtcagaa
     3181 tgcggcctgg cgcaggctgg gtgcgcgctg gcagctcttg tttctgtagc tttcgggggc
     3241 tggcggcggg gctggagttc ttgcgccgcg gggactggta ggcggggctg ccggccggtt
     3301 gccaggccgg gatgaggtgg tgcttgccgc tgccgatcag gtcgtggcgg cccatgtcct
     3361 tgagtgcctg gcgcagcagg ggccagttgg ccgggtcgtg ccagcgcaga aaggccttgt
     3421 gcaggcggcg ccggcgttcg cccttgacgg tgtcgacccg ctcgccgcga ccgtcgcggt
     3481 gcacgccctt gagcgggttg acgccggtgt ggtacatggt ggtggcggtg gccatggggc
     3541 tggggtagaa ggcctgcacc tggtcggcgc gaaagccgtt gcgcttgagc cagagcgcga
     3601 gctggagcat gtcctgatcg ctggtgccgg ggtgggcggc tatgaagtac gggatcaggt
     3661 actgctgctt gccggcttcg gcgctgaagc ggtcgaacag ctggcgaaag cgctcgtagt
     3721 cgccgatgcc gggcttcatc attttcgaca gcgggccttg ctcggtgtgt tcgggcgcga
     3781 tcttgaggta gccgccgacg tggtgttgca ccagttcctt gacgtaggcg ggcgagcgca
     3841 ccgccaggtc gtagcgcagg ccggagccga tcaacacctt cttgacgccg ggcagctggc
     3901 gcacgcggcg gtagacgcgg atcagcgggt cgtggtcggt gcgcaggttg gggcagatgc
     3961 cggggtagac gcagctgggt ttgcggcaca ccgcttcgat ggccgggtcg cggcagccca
     4021 ggcggtacat gttggcggtg gggccgccga ggtcgctgac gacgccggtg aagccctgca
     4081 ccttgtcgcg catggcttcg acttcgcgca cgatggagtc ttcgctgcgg ctctggatga
     4141 tgcggccttc gtgctcggtg atggagcaga aggtgcagcc gccgaagcag ccgcgcatga
     4201 tgttgacgga aaaacggatc atttcccacg ccgggatgcg ggtggcgccg tcgtggcggc
     4261 cgcgttcgtc ggcgtagcgc gggtgggggt tgcgcgcgta gggcagatcg aagatgtggt
     4321 ccatctcggc ggtgctgagc gggatgggcg gcgggttgat ccacacgtcg cgcgccgaac
     4381 gcccctcgcc atgcgcctgc accagcgcgc gggcgttgcc ggggttggtc tccaggtgca
     4441 gcacgcggtt ggcgtgggcg tacagcacgg ggtcgcgccg cacctgctcg tagctgggca
     4501 ggcggatcac gctgcgggcg tggtcggggc ggatcagggc gatcggcttt tcaaggcctt
     4561 tttgggctgc agcgcttgtc ggatcagcgc cagcagcttc tgtttttgta gcgtcgcaat
     4621 ggctgccctg cgcggcggcg cgttcggcgg tggtctggta ggggctgctc agggcgtcca
     4681 cccggccggg gcgatcgacc tcggtggagt cgatttcgcg ccagcccggc gccgccgtgc
     4741 ggcgcacgaa ggcggtgccg cgcacgtcgg tgatctggcc caccggttcg cgccgcgcca
     4801 ggcggtgggc gatctcgacc agcgcgcgct cggcgttgcc gtacagcagc aggtcgcact
     4861 tgctgtcgac cacgatggag cggcgcacgc tgtcgctcca gtagtcgtag tgcgcgatgc
     4921 ggcgcaggct gccttcgatg ccgcccagca ggatgggcac gtccttgtag gcctcgcggc
     4981 agcgctggct gtagacgatg gcggcgcggt cgggccgctt gccggccacg tcgccggggg
     5041 tataggcgtc gtcgctgcgg atcttgcggt cggcggtgta gcggttgatg agcgagtcca
     5101 tgttgcccgc cgtcacgccc cagaacaggt tgggccgccc cagggccttg aacggctcgc
     5161 tgctgtgcca gtcgggctgg gcgatgatgc cgacgcgaaa gccctgcgct tccagcacgc
     5221 ggccgatcac ggccatgccg aagctggggt ggtcgacgta ggcgtcgccg gtgacgatga
     5281 cgacgtcgca cgaatcccag cccagcgcct ccatctcggc gcggctcatg ggcaggtagg
     5341 gcgcggggcc gaagcgcttg gcccagaagg gtttgtagcc ggtcagcgca ggcgcgcgca
     5401 gcggcagggt ggcgacggtg ctcatggcgc ggctggcggg ttggcggcca gggtgcggcg
     5461 gctcggcgtc atgccgccag cgcccttcag gcgattgcag aaacggtagc gcctcgcggt
     5521 cgattgcacc ggcttgcgcc ccatgggttg gcccaagcgt ccgaggcgtc gggaggccgg
     5581 cccattttac cgaccccggc cgctggtcgc tcgccggcgc cggcgggcgc tcacttccgc
     5641 tcgatcagct ggccgcgcag ctcgccttcg gggtaggcgg cggtgcgcag attgacgtac
     5701 cactggcccg acagcaggtc ggcgcgctgg cgtggcgtga gcatggcgcg gccttccagt
     5761 ggactgacca cggtgcggcc cagcgtcagc accgggtcgg ccacctcgcc ggccatgccg
     5821 gggctgtgaa agtgcgccgc gcgcaccggg ccgctgaggc cgctgaagct cagcttccac
     5881 tgcagcaggc cggtgttgcg gttgagcacc gccaccagtt ccccctgccc ggtgctggcg
     5941 ctgggtggca cggcctggcg gccgctcagc tgggcgctga aggcggccag ttcgggcagg
     6001 cgcggcggcg cgctgggcgt ggcccgcggc cggggcgcgg gcggcgggct ggcgcagccg
     6061 gccagcacgg ccagcagcgc cgccgccacg gccaggcggg tggcggcggg gatcgacgcg
     6121 acgtgcgcgc gcgcgggctg gggcaagcgg aacatcaggc gcaccgtagc atccattggg
     6181 cggcacgtgg tgggcattca cccatggata cctcggtgtc ggcagagata cgtcctgcac
     6241 gcgcccggct cagcacacac acaccgcatc agggcaccac ccgcacctgc ccgcgcagct
     6301 cgcccagcgg ataggcggcg gtgaagacgc tgacgtacca gcgccccgcc agcaggtcca
     6361 tggcctgcgc ggcgctgagg ctggcgcggc cttcatagga cggcccgaaa ggcgccggcc
     6421 acaccagcac ggccggcgcg ttctgccccg catcggccgg gccgtgaaac tgcacgccgg
     6481 tgatggcgcc gctcaggccc gaccagcggg tcttccagcg cagcaggcgg gtgtcggggt
     6541 cgtagatggc gtcccactga ccgctgccgg cgctgcgcgt gggtggcatg acgctggcgc
     6601 cgtccagccg cgtgctcagc gtcaccaggc gggcgctggc gtcggtcggc ggcaggggcg
     6661 cgctctgccc ggccggcggc agcggctgcg gcactggctg ggcgggcggt accggctgcg
     6721 tctgcacccc gccgggctgg gccggcggca cctgcgccgg cggcaatggc tgcggcatgc
     6781 ggatcggggg cgggtcgtag cgccggccca gatcgaccga ggcgcaggca ctgagcaggc
     6841 cggcggccac cagccccgcc agcacggcgt gcgaacggtt ccagtgcggc aaagacatcg
     6901 gcagactcct ccagaacgac aacgacaggt ggcggccggc gcgcggccgg tgggcgtcga
     6961 ttgtaggcag ccgcgccggg cgggcatgcc ggctggcgcg ccgcaggccc gctgcgcccc
     7021 tgctatgctg gcccgcccgc catcgcccca ggccgcgcca tgcccacact cgatctgcct
     7081 gcgctgcaac agctcatcca acccctgctg cccggcctgc tgggtgtgcg cctgaccgaa
     7141 gccaccgccg agcgcgtgcg cgccgaaatg acggtgcgcc ccgagctgtg caccaccggc
     7201 catgtcctgc acggcggctc gcacatggcg ctggccgaca ccctgggggc gatcggcacg
     7261 gtgctcaacc tgcgacccgg ccagcgcacc gtgaccaccg attccagcac caagttcatc
     7321 gccggcgcgg ccgaaggctc ggtggtcacc gccgaagccg tcgccctgca ccgcggccgc
     7381 agcacccagg tgtggcagac caccatccgc aacgccgaag gcaggctgtg cgcggtggtc
     7441 acgcagaccc agctggtgct ggcgacccca ggcgagtgat tgcaggccga gcgccgccat
     7501 gtcccagccc ctgagccgcc accggctgca cccacacccg agcacgccgg caccggccgt
     7561 gcgcagcctg tgcgtggacg tcggccgcag cgacacggcg cccggcgcgt ggtggctgcg
     7621 ctttcggctg caggcggcgc tggaggcgct ggcgctgccg ccgcccggcg cgtctgccgg
     7681 gccggcggac gacctgtggc gccacacctg cttcgaggcc ttcgtcggcc gcgcgcaggc
     7741 tgcggcctac cacgaattca acttcagccc tgcgggcgac tgggcggcct acgccttcag
     7801 cggcccgcgc cagcgcgcca gcgccgacca gctgccactg gcgccgcgcc tgcagtggca
     7861 gcgcgccgac gacaccctga ccctgaccgt cgagctgccg cgccaggctc tgccggccgc
     7921 cgaccacacc gacctcctgc tgggcctgag cgccgtgatc gaagcgcgtg acggccagct
     7981 cagctgctgg gcgctggccc acccggggcc gcagccggac tttcaccacc gcgccggctg
     8041 gacggcgcgc ctgccctgat ctgcctcaca tgaaattcgg actcgaacgc tttcttgaag
     8101 agcccttgct gcgcgcgcca ctggcgggcc ggcgcgtggc gctgctggcg cacccggcct
     8161 cggtcacgcg caacctgacg cacgcgctcg acgccctggc cgccctgccc gatgtgcgcc
     8221 tgacggcggc cttcggcccg cagcacggcc tgcgcggcga caagcaggac aacatggtcg
     8281 agtcgagcga ctacaccgac ccgcgcctgg gcatcccggt gttcagcctg tacggcgagg
     8341 tgcgccgccc caccccggcc atgctggaga cgtttgacgt gctgctggtc gatctgcagg
     8401 acctgggctg ccgcatctac accttcatca ccac
//
LOCUS       DAOOOG010000115         8346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_10017_length_8346_cov_28.105898, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000115 DAOOOG010000000
VERSION     DAOOOG010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10017_length_8346_cov_28.105898"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PK242_09160"
     CDS             complement(<1..311)
                     /locus_tag="PK242_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOZ94228.1"
                     /translation="MRAPLRHQLQRHAERLAELDFLLAQPDVQADTEQLMALSREHTE
                     VAAMAGRWARHQQRERDLAAAQELLGDADMADMAREEVAACQAELAALEAELQRLLLP
                     K"
     gene            complement(393..824)
                     /locus_tag="PK242_09165"
     CDS             complement(393..824)
                     /locus_tag="PK242_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HOZ94229.1"
                     /translation="MRRHAPTARPTGFTLIEVMIVVAIVAILAAIALPSYNEYIRRGH
                     RADARAGLLQAQQWLERAATATGVYPTSLPASLTWSGDGTKRYDIALAAGATNAAFTL
                     TATPKGAQVGDKCGTYTLTHTGLRGANGVTSGAIVTECWGK"
     gene            complement(836..4453)
                     /locus_tag="PK242_09170"
     CDS             complement(836..4453)
                     /locus_tag="PK242_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOZ94230.1"
                     /translation="MPGQALCPSRRFRPSLLALAACAALLPHGAWSLTLVQSPPGAQQ
                     PYVAPNVIISVDDSGSMDWKLINQSTGSSATGPGYTQPYPDGSWNTSAKRINILKFSL
                     NKIFTDTTLLPDGKIRVAWQTMWNNGGAPGVGPSKSGKPAGATSVNSTTSGVNSMKVL
                     QGAHRTNFLSFVSSLTPGGNTPAHWMFEQADGYMRQPLGVNSPWASVPGTTAGPYLGC
                     RRNYHIMMTDGRWNSSPSGGQRDGVNSLTLPDSTVYADGTAAQIAKTRVFRDTASNTL
                     ADWAFRSWSDPLQVAASLTGSLQPTADYLKAPATESFGNDSAGNPAVLDRYWNPRYNP
                     ANWPHMVTYTIGASSDATTWPGAPTIFGPTAKVPYGYDGSFPDFVTGNKTWPDMGNGE
                     PVRALDLWHASINGRGRFYAVNKAEDMEQAFRDIFEQINALVEPGTGSTAASGASITR
                     NEVGLFTAAYNPKDAWKGYVAAQKVLKDGSIVSHPGWGGQSSAVKLDALAGVNNRLIL
                     SWNDKWASSPAPGRPTGGAPFKWTGDESNLSAAQKLWLQRNPSDNTDQGATKGQQRLD
                     YVRGDRSLEGVDPGGYTSAKPFRQRHSRQGDIINSDVWYVGAPASNYPLKGYLQFTDA
                     RKARTPMIYVGGNDGMLHGFSADDGIEKIAYVPRGVIPTLNQLTNPAYNNAHRYYVDG
                     SPMSGDIDLGMTPGGNDTDSSYTPNWRTMLVGTLGAGGKGYFVLDITDPSIFSESNAS
                     ALARLDRTRGSTEPAPNCAIITDTAEKAACTLAVQEDADIGHITAKPVRDEANGMRVT
                     QITRMNNNRWAVVLGNGYNSANQRPVLIVQYLDGDRKLKLIPATQDAPGTGKAADNGL
                     ASPRVVDLNGDGRTDIVYAGDNQGNLWKFDLTSASDADWDVAFGGSATSKPLFTAKGP
                     ASLSATTRPKVQPITAAPTVRANDRMMLADPADPSSLTPVGGMMVAFGTGRNVAQNDA
                     ESVDVQTLYSVLDNTRYRYRSPVPSIGKRLEVHPGGGTCPNGSDCVPAPAALGTMGNT
                     GNLSSGGQLAKQKITDVGTEFGTVDAVQELKKETWANFHGWFLDFPAVGERQLKPIEF
                     FDGSNILTVYSQVPGRGSDVDPTIEQCDVPSLFSERQFRTLVNIMDGKRPSVQLVDMN
                     GDNVYTAADQGVSRREVGGGSHTLVTQGKNVLDIDVKNQKERLARMPEQSLRPSWRQL
                     K"
     gene            complement(4546..5355)
                     /locus_tag="PK242_09175"
     CDS             complement(4546..5355)
                     /locus_tag="PK242_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOZ94231.1"
                     /translation="MIRTPTLHAHHRSSPGRQRGVALFVVIVFVLLSMLLALWASRTS
                     MFNELVVGNDADHQRAFEAAQALLQDAELDIRGERADGFACDADDVSAQPNEVCRRGN
                     VTKMPLEAQELSPLLAYLNQQTVRCSHGLCARRTGRQDFWNNTTVNNSTTGEFPLSTL
                     TVNAVGARYGQYTGALAGNASTPANPILADTSADNRGGWYWIEVLPYDESSKSANLIP
                     GTGTGLLPLNLTPSVVYRVTALAYGRKTNISGDPVTMVVLQETYAPTRRKD"
     gene            complement(5367..6230)
                     /locus_tag="PK242_09180"
                     /pseudo
     CDS             complement(5367..6230)
                     /locus_tag="PK242_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
     gene            complement(6326..6988)
                     /gene="pilV"
                     /locus_tag="PK242_09185"
     CDS             complement(6326..6988)
                     /gene="pilV"
                     /locus_tag="PK242_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HOZ94232.1"
                     /translation="MQSHSTHRQRGITLIESLVAILVTALGILGILGVQMRTLTDTQT
                     TVRRAQAIRLIEDLGERLKVNPNALMVLGSYQSGWTANPPTPPAGTNCASVACSPAQL
                     AAYDVAEWKRLVQRSLPLGDASIFISDAEAGISNNRRQLGVMISWRENEQTGLSTSDK
                     ENTDATQVRAYDGTLSAGAGSAATCPADRSCQLQFIPVAARCAPDLREGAANPKFFCA
                     GS"
     gene            complement(6994..7569)
                     /locus_tag="PK242_09190"
     CDS             complement(6994..7569)
                     /locus_tag="PK242_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HOZ94233.1"
                     /translation="MEHPAIAPHRTAPAAARGFTAIELMVVVAIVAVLAALAAPSFTP
                     LIERWRVRQAAEGLESTFYYARSEAIKRGGGVVVQKLPNGTNGCVASGTADWNCGWIV
                     CEDTNGNGSCGAAEPVLQRFDAPARIEVSRTSNGASIPLNRWGLIAGTFIGFNLVPAG
                     KGISDPSVRGVCISSGGRVRIIPSDQVPCTG"
     gene            complement(7796..>8346)
                     /locus_tag="PK242_09195"
     CDS             complement(7796..>8346)
                     /locus_tag="PK242_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOZ94234.1"
                     /translation="SCTASQLPIIGLGAVERALKKRRHRPIFMVDLAVPRDIEPEVKG
                     LDDVYLYTVDDLAQVVHTGQANRQAAVAQAEAIIDAGVQSFMHWLDQRDPAGGVVPLI
                     QQLGAQADEWRAIELARARKRLARGEDLDAVLDALSRGLTQKMLHGAMAELNATDPAH
                     REQTSDAIARLFLRGKLPTQSH"
BASE COUNT         1254 a         2537 c         2957 g         1598 t
ORIGIN      
        1 tccttgggca gcagcaggcg ctgcagctcg gcctccagcg cggccagctc ggcctggcag
       61 gcggcgactt cttcgcgcgc catgtcggcc atgtccgcat cgcccagcag ctcctgcgcc
      121 gcggccaggt cacgttcgcg ctgctgatgg cgcgcccagc gcccggccat cgcggcgacc
      181 tcggtgtgct cgcgcgacag cgccatcagc tgctcggtat cggcctgcac gtcgggctgg
      241 gcgagcagga agtccagttc ggccaggcgt tcggcgtggc gctgaagttg gtggcggagg
      301 ggggcgcgca tgaaaacacg gaaaccggcg attttcgccg gtttgttgac cgcacggttc
      361 ggcagcgctt cagccggagc ggggatgcgc ggctacttgc cccagcattc ggtcacgatg
      421 gcgcccgaag tgacgccatt ggccccgcgc aggccggtgt gggtgagcgt gtaggtgccg
      481 catttgtcgc cgacctgggc gcctttcggg gtggcggtca gcgtgaacgc ggcgttggtt
      541 gcccctgcgg ccagcgcgat gtcgtagcgt ttggtgccat cacccgacca ggtgagggat
      601 gccggcagcg aggtggggta gacgccggtg gcggtggcgg cgcgctccag ccactgctgc
      661 gcttgcagca ggccggcgcg tgcgtccgcg cggtggccgc ggcgaatgta ctcgttgtag
      721 ctgggcagcg cgatggcggc caggatggcg acgatggcca ccacgatcat cacctcgatg
      781 agcgtgaagc ctgtggggcg cgcagtaggt gcgtgtcggc gcattcggtg ctccttcact
      841 tgagttgccg ccagcttggg cgcagcgact gttcgggcat gcgggccagg cgttccttct
      901 ggttcttcac atcgatgtcg agcacattct tgccctgggt aaccagggta tgcgagccac
      961 cacccacctc tcggcgcgac acgccctgat cggcggcggt gtagacgttg tcgccgttca
     1021 tgtcaaccag ctgcaccgag gggcgcttgc cgtccatgat gttgaccagt gtgcggaact
     1081 gacgctcgct gaacaggctc gggacgtcgc attgctcgat cgtcgggtca acgtccgatc
     1141 cgcgcccggg aacctgcgaa tacaccgtca ggatattgct gccgtcgaag aattcgatgg
     1201 gcttgagctg gcgctcgccc acggcgggga aatccaggaa ccagccatgg aagttcgccc
     1261 aggtttcctt tttcagttcc tgcacggcat cgaccgtgcc gaactcggtg ccgacgtcgg
     1321 tgattttctg cttggccaac tgtccgccgc tgctcaggtt gccggtattt cccatggtgc
     1381 ccagtgctgc gggggctggc acgcagtcgc tgccgttggg acaggtgcca ccaccgggat
     1441 gcacctccag gcgcttgccg atggagggga cgggcgaacg atagcggtag cgggtattgt
     1501 cgagcaccga gtacagggtc tgaacgtcca cgctctcggc atcgttctgg gccacgttgc
     1561 gaccggtgcc aaaggccacc atcatgccgc ccaccggtgt cagcgaggac ggatcggcag
     1621 ggtcggccaa catcatgcgg tcattggcgc gcacggtggg ggctgccgtg atcggctgca
     1681 ccttggggcg cgtggtggcc gatagcgacg ccgggccttt tgccgtgaac agcggcttgc
     1741 tggtggccga gccgccgaat gcgacatccc agtcagcgtc gcttgcgctg gtcagatcga
     1801 atttccacag gttgccctgg ttgtctccgg catagacgat gtcggtacgg ccatcgccgt
     1861 tcagatcgac caccctgggc gaggctaggc cgttgtcggc cgccttgccg gtcccagggg
     1921 catcttgtgt ggccggaatc agcttgagtt tcctgtcccc gtcgaggtac tggacgatga
     1981 ggacgggccg ttgattggcg ctgttgtagc cattgccgag taccaccgcc cagcggttgt
     2041 tgttcatgcg cgtgatctgg gtgacccgca tgccgttggc ttcgtcgcgc accggcttgg
     2101 cggtgatgtg gccgatgtct gcgtcctcct gcaccgcgag ggtacaggcg gccttctcgg
     2161 ctgtgtcggt gatgatggcg cagttcggtg caggctcggt gctgccgcgc gtccgatcca
     2221 gcctcgccag tgccgacgca ttggattccg aaaagatgct gggatcggtg atgtccagaa
     2281 cgaaataccc cttgccacct gcccccaggg tgccgaccag catggtgcgc cagttggggg
     2341 tgtaggaact gtcggtgtca ttgccgcctg gcgtcatgcc gagatcgatg tcgccgctca
     2401 tcggtgagcc gtcgacatag tagcgatgtg cgttgttgta ggcagggttg gtgagttggt
     2461 tcagtgtcgg aatcacgccg cgcggcacgt aggcgatttt ctcgataccg tcgtcggccg
     2521 agaagccgtg cagcatgcca tcgttgcccc cgacgtagat catgggggtg cgagccttgc
     2581 gcgcgtcggt gaattgcagg taacccttga gcgggtagtt gctcgccggt gcgcccacgt
     2641 accacacgtc cgagttgatg atgtcgccct gacggctgtg gcgttgtcgg aagggtttgg
     2701 cggaggtgta tccacccggg tcgacgcctt ccagagagcg gtcgccgcga acgtagtcca
     2761 ggcgttgctg gcccttggtg gcgccctggt cggtgttgtc gctggggttg cgctgcagcc
     2821 agagtttctg cgccgcgctg agattgctct cgtcgccggt ccacttgaac ggcgcaccac
     2881 cggtgggcct gcccggcgcg ggactgctcg cccatttgtc gttccagctg agtatcaggc
     2941 ggttgttgac gccagccagt gcatccagct tgacggccga gctctgtccg ccccagccgg
     3001 gatgcgagac gatgctgccg tccttgagca ccttctgcgc cgccacatag cctttccagg
     3061 catctttcgg gttgtaggct gcggtgaaca gacccacctc attgcgcgtg atgctggccc
     3121 cgctggcggc ggtcgatccg gttcccggct ccaccagggc attgatctgc tcgaagatgt
     3181 cgcggaaggc ctgttccatg tcctcggcct tgttgacggc gtagaagcga ccacggccat
     3241 tgatggacgc atgccagaga tcgagcgccc ggacgggttc tccgttaccc atgtccggcc
     3301 aggtcttgtt gccggtgacg aagtcgggaa aactgccgtc gtaaccgtac ggcaccttgg
     3361 ctgtcggccc gaagatggtg ggcgccccag gccaggtggt ggcatcgctg ctggcgccga
     3421 tcgtgtaggt caccatgtgt ggccagttgg ccgggttgta gcgtgggttc cagtagcgat
     3481 ccagcacggc cgggttgcca gcgctgtcgt tgccgaagct ttcggtggcg ggggccttca
     3541 ggtagtcggc cgtgggttgc agggatccgg tcagcgatgc cgccacctgc agcgggtcgg
     3601 accagctccg gaaggcccag tcggccaggg tgttcgacgc ggtgtcccgg aacaccctgg
     3661 tcttggcgat ctgggcagcc gtgccgtcgg catagaccgt actgtcgggc agcgtgaggc
     3721 tgttgacgcc gtcgcgctgt ccaccgctgg gcgagctgtt ccagcgaccg tcggtcatca
     3781 tgatgtggta gtttcgccgg caacccaggt agggtcccgc ggtggtgcct ggtacgctgg
     3841 cccatggact gttgacgccc aggggctgac gcatgtagcc atcggcctgc tcgaacatcc
     3901 agtgagccgg ggtgttgcca cctggggtca ggctgctcac gaagctcagg aagttcgtgc
     3961 ggtgcgcgcc ctgcagcacc ttcatcgaat tgacgccgct ggtggtgctg ttgacgctgg
     4021 tggcgccggc cggcttgccg ctcttgctag gtcccacccc aggtgccccg ccgttgttcc
     4081 acatggtttg ccaggccacg cgaatcttgc cgtcgggaag cagcgtggtg tcggtgaaga
     4141 tcttgttgag cgagaacttg aggatgttga tgcgcttggc actggtgttc caactgccgt
     4201 cagggtaagg ctgggtgtag cccggccctg tcgcactgga cccggtggac tgattgatga
     4261 gtttccagtc catgctgcct gaatcgtcca ccgagatgat gacgttgggc gccacatagg
     4321 gttgctgagc gcccggcgga gactgcacca acgtgagcga ccaggcgccg tgcggcagca
     4381 gggccgcgca ggcggccaga gcaagcaggc tggggcgaaa acgacgtgac gggcagaggg
     4441 cttggccggg catggcgaac tcctgtctgg gcaatatgtc ttcggtgtga gggcgacggt
     4501 tcagatgaac gtgatcctgg ggcagccttt gggcctggcg ggtgctcagt ccttgcgccg
     4561 ggtcggcgca taggtttcct gcagcacgac catggtcacc gggtcgccgc tgatgttggt
     4621 cttgcggccg taggcgaggg cggtgacgcg atacacgaca ctgggcgtga ggttcaaggg
     4681 cagcaacccg gtgccagtgc ctgggatcag gttggcgctc ttgctgcttt cgtcgtaggg
     4741 cagcacttcg atccagtacc agccgccacg gttatcggcg ctggtgtctg ccaggatcgg
     4801 attggcgggc gtgctcgcgt tgccggcgag ggcgccggtg tactggccat aacgggcacc
     4861 cacggcgttg accgtcaggg tgctcaacgg gaactctcct gtcgtcgaat tgttgacggt
     4921 ggtgttgttc cagaaatcct ggcggccagt ccgacgcgcg cacaagccat ggctgcaacg
     4981 aaccgtctgc tgattcaggt aggccagcag ggggctaagt tcctgcgctt cgagcggcat
     5041 cttggtcacg ttgccgcggc ggcagacctc attcggctgc gcgctcacgt catccgcgtc
     5101 acaggcaaag ccgtcggcgc gctcgcctcg tatgtcgagt tcggcgtcct gcagcaaggc
     5161 ctgtgccgcc tcgaacgcgc gctggtggtc ggcatcgttg ccgaccacca gttcgttgaa
     5221 catcgaggtg cgcgaggccc agagcgccag cagcatggac agcagcacga acacgatgac
     5281 gacgaacagc gcgacgccac gctggcgccc aggactggaa cggtggtgtg catgaagcgt
     5341 cggggtgcga atcatggctt cgcctttcac atcaacccct ggctgcgcaa ctggaacacg
     5401 ttacgaaaca ccaggtgcag tcggttcctg cgtggcggat tgctggtgct gagggtggtc
     5461 atgttcacct gcgtgacgcc gtcgcagtcg gtgtagctgg tgcccgctgg catgtcgatg
     5521 gactcgtcgc cgtacagcac cagacagacc tcgacgccct gtacctgccc ccaattggct
     5581 acgttggccg cggccacgcg gcgcagggtg ggattgccca gcgaggtggt ggcgtcctgg
     5641 accagatagg taagctggaa gttggccacg cggtcgacga tggcctgtgc cggtgcgacg
     5701 ccattgccct ggcagcggag ttcattggag accagggtga agatgctctc gatgcgctgg
     5761 tcgacgctgg cgttgcctgg cgcaccgacg caattgcgtg cgagcgccat gtcgggatcg
     5821 agcggggtcg cgggatccgg gacgaacacg ggctccttgt agcggcgaaa acccagggtc
     5881 aggctgttcg ccgtgccgtt cagcagcttg tctcgctcgg ccgggatatc caggtcgaag
     5941 tggtaggttc ccgatgcagc ctcggccttt ttctcgaacg ccacaggcgt tagtggatcc
     6001 acgccccctg gcgcattgcc ggggttcgga ttgagataca gcgaagcggt ctgccgcgcc
     6061 tgcaaaccaa tgacgcgcat cgcataggcc gcctgctgct ggatcttgct ggcatcgccg
     6121 accgtgccgg tgacgccgcg cgagaccata agtgcgcctg tggcgacagc caccaccatc
     6181 aggccaatgg cgatgcccac cagcagttcg atcaggctca cgccgcgctg ggtatgcggt
     6241 ggcctgaatg cccggctggg gtgaccgcgg ggtgaccagg tgcttgcacc tgcggtgcgg
     6301 gcaatgtggc gcttgttggt catgatcagg aacccgcgca gaaaaatttc ggattggccg
     6361 cgccttcgcg cagatcgggg gcgcaacggg cagcgacggg aatgaactgc agttggcaac
     6421 tgcggtcggc ggggcaggtg gcggcgctgc cggcacctgc gctcagcgtg ccgtcgtacg
     6481 ctcgaacctg tgtggcgtcg gtgttctcct tgtcggaggt gctcagaccc gtctgttcgt
     6541 tctcgcgcca gctgatcatc acgcccagct gtcgccggtt gttacttatg cctgcttcgg
     6601 catccgaaat gaagatgctg gcgtcgccca aaggcaggct gcgctgaacc agacgcttcc
     6661 actcggccac gtcataggcc gccagctggg ctggggagca ggcgaccgat gcgcagttgg
     6721 tgcctgcagg aggcgtggga gggttggctg tccaaccact ctggtagctg ccgagcacca
     6781 taagcgcgtt ggggttgacc ttgagacgct cgcccaggtc ttcgatcaac cgaattgcct
     6841 gggcgcggcg cacggtggtc tgggtgtcgg tcagggtgcg catctgcacg cccaggatgc
     6901 ccaggatgcc cagggccgtg accaggatgg ccaccagcga ttcgatcagg gtgatgccgc
     6961 gctgtcggtg tgtgctgtgg gattgcatga cgatcacccg gtgcagggca cttggtcgga
     7021 ggggatgatg cggacgcgac cgccggagct gatgcatacg ccgcgcaccg aggggtcgga
     7081 gatgcccttg cctgcgggca ccaggttgaa gccgatgaag gtgccggcga tcaggcccca
     7141 gcggttgagg ggaatgctgg cgccattgct ggtacgggaa acctcgatcc gagcgggagc
     7201 atcgaagcgc tgcagcaccg gctcggcggc gccgcagctg ccgttgccgt tggtgtcttc
     7261 gcagacgatc cagccgcagt tccagtcggc ggtgccgctg gcgacgcagc cattggtgcc
     7321 gttgggcagc ttctgcacca ccacgccgcc gccccgcttg atggcttcag agcgcgcgta
     7381 atagaaggtg gactcgagac cctccgccgc ctggcgcacg cgccagcgct cgatcagggg
     7441 ggtgaagctg ggggccgcca gcgccgccag gacggcgacg atggcgacca ccaccatcag
     7501 ttcgatggcg gtgaagccgc gtgcggcggc aggcgcggtg cggtgcggcg caatggcggg
     7561 atgttccatg acgccatgct agggcgggca tggcgctggt gggaccggcc tcgggacgaa
     7621 cggcaggccg cggccgatcg gcggtcgccg tgaccggcgc agtgtgaaga attccagcag
     7681 gagagctccc gccctattga tccggccctt ggaagtctca gattccgttt gggctgagcc
     7741 tgtcgaagcc cttcgacagg ctcagggcga acggtcaaaa cctcatgggg cccgatcaat
     7801 gggactgcgt gggcagcttg ccccgcagaa acagccgcgc aatggcgtcg ctggtctgct
     7861 cgcggtgggc cggatcggtg gcgttcaatt cggccatggc gccgtgcagc atcttctgcg
     7921 tcagcccgcg gctcagggcg tcgagcacgg cgtcgaggtc ttcgccgcgc gccaggcgct
     7981 tgcgcgcgcg cgccagctcg atggcgcgcc attcgtcggc ctgggcgccc agttgctgga
     8041 tcaggggcac cacgccgccg gccgggtcgc gctggtccag ccagtgcatg aagctctgca
     8101 cgccggcgtc gatgatggcc tcggcctggg ccacggcggc ctggcggttg gcctggccgg
     8161 tgtgcaccac ctgggccagg tcgtccacgg tgtagaggta gacgtcgtcc aggcccttga
     8221 cttcgggttc gatgtcgcgc ggcaccgcca gatcgaccat gaagatgggg cggtggcgac
     8281 gctttttcag cgcccgctcg acggcgccca ggccgatgat gggcagctgg ctggcggtgc
     8341 agctga
//
LOCUS       DAOOOG010000116         8220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_10229_length_8220_cov_28.699571, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000116 DAOOOG010000000
VERSION     DAOOOG010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10229_length_8220_cov_28.699571"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PK242_09200"
     CDS             <1..1130
                     /locus_tag="PK242_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOZ94235.1"
                     /translation="EMNTRLQVEHPVTEAITGQDLVEWQLRVAAGEPLPLAQDELLID
                     GHAIEARICAENPDNHFLPATGTLHVYRKPPHAAFEFGAVRFDDGVREGDAISPFYDS
                     MIAKLIVWGETRQQALAKLDAALAATHIAGLQTNVQFLRHVLASDSFARARLDTALIE
                     RERPRLFQQDRLGRDLAVAAAVAQTVLAERAAETHDPFSRTDGFRSHGVATRRVDLEY
                     RGEAVVAKLRYLQDGLMLQIGDAEAQPLSLTALPDGRIDLRYGTARELVQVYPLGEVE
                     HVFGSRGAVQITELDLLSRAEEGAGEGGRLTAPMPGKVVSIAVRAGDKVSKGQALAVM
                     EAMKMEHTIAAPQDGVVDEVLYAPGDQVAEGAELLRLSHPG"
     gene            complement(1203..2681)
                     /locus_tag="PK242_09205"
     CDS             complement(1203..2681)
                     /locus_tag="PK242_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/universal stress protein"
                     /protein_id="HOZ94236.1"
                     /translation="MSALEPGAEIDGFLIGEMIHKGGMALIYETRYADGRAAPFPMVM
                     KVPRMTAGDGAETIVSFEVEHQMMQALDGPHVPYLVAAGDLSVLPYLVMEYVPGRTLD
                     HWLDQVLQGQPLPPHELARLGAAVARAAHDMHQQNAVHLDLKPANVIIRDAAEGAPAD
                     APLHAVLLDFGLSFHAHYPDLLAEELREAVGSPAYMSPEQVVGVRGDPRSDVFAIGVM
                     LYELATGELPFGAPSSKGGLRQRLWMDPKPPRARRPDLPEWLQEVILRCLEPVAAERY
                     PSAAHLAFDLAQPEQVQVTERGRRLKGTGFRTHFKRWLRAAGTQYQPSPLPTRVIDEV
                     PILMVAVPHHDVTDATLYSLREAVRRSLGIRPGARLAVVTVVAPGAASSTDASRSETT
                     QHRQMLTMLKTWAQGLPQEQHQISYHVLEDGDPADAILRFARANHVGMIVMGAATHGL
                     QLQRFVATVPIKVAMDAPCTVILVKQSMPFEQLGAVPASLSA"
     gene            complement(2678..3442)
                     /locus_tag="PK242_09210"
     CDS             complement(2678..3442)
                     /locus_tag="PK242_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOZ94237.1"
                     /translation="MRLALLSDLHANRQALDACLAHAQAAGAERLAFLGDLVGYGGDP
                     AYVVQRVMDLAAEGALVVKGNHDVYATDAAGDGKTLGQAGALWTQEQLSAAQRRFLAE
                     LPLTLHQPPLLLVHASAAAPERWHYVETPLLAALSLDAACEPPVGGPDVHYVFGGHVH
                     RQTLYYRGGTGKLMPFTPTAGVPVPVPRHRRWLATVGSVGQPRDGRTDAMYALFDDVA
                     QQLTFHRVPYDVPSAAGAIRATPLPPFFADRLEQGR"
     gene            3563..4471
                     /locus_tag="PK242_09215"
     CDS             3563..4471
                     /locus_tag="PK242_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOZ94238.1"
                     /translation="MSLPTQVRLIDVGPRDGLQNEKQPVPAAVKIELVQRLQAAGLRE
                     IEVTSYVSPKWVPQMADNHQVMGGIARQPGVRYSVLTPNLKGFEAALADRPHEIVVFG
                     AASEAFSQKNINCSVAESIERFAPVVAAARAAGIAVRGAMSCIVGCPYEGDIAPEKVR
                     QLAGLMKGIGVERVDVADTIGVGTPRKVQRAIEATLAHYDIDHVSGHFHDTYGQALAN
                     TLAALELGVWNFQSSVAGLGGCPYARGATGNVASEDLVFMLHGMDIATGIDLDRLIDA
                     GQFISQHLGRPPNSRVATAVLNKRAG"
     gene            4506..5000
                     /locus_tag="PK242_09220"
     CDS             4506..5000
                     /locus_tag="PK242_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOZ94239.1"
                     /translation="MNHPTSPALPDAMRRVAAVLAAHGHPHAPILLDDAARTAQQAAD
                     ALGVVVGQIAKSIIFRRRSDDAAVLVIAAGDRRVDEKKVAALVGPVGRADADFVKART
                     GFTIGGVSPVAHASPPVVLIDRELYRFDVIWAAAGHPHGVFRLAPADLERLTGAPVAD
                     VAQG"
     gene            5007..5294
                     /locus_tag="PK242_09225"
     CDS             5007..5294
                     /locus_tag="PK242_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HOZ94240.1"
                     /translation="MAPVPRDFDTLARQAQQALRDGTPLPSPCIGVCRIGDAGVCVGC
                     ARRLDEIGGWLHADEAGRGAIWRRVLQRRGLRAGAGGAAPDPARLAQGEQR"
     gene            5407..7227
                     /locus_tag="PK242_09230"
     CDS             5407..7227
                     /locus_tag="PK242_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HOZ94241.1"
                     /translation="MPNAFNFNASPFDSLTPQEQRLVRDHVDVVYFRPDEVILDAGIA
                     PMHLFVIIKGVVQQFDGEELVATYGPDDSFDGRALVAGRASSRFVAVEEVVAYALARQ
                     TVNDLIAANATFGALVFSDLGAKLSAASQRHSGNELQALNMARVDEAFVRPAHLVEAD
                     TDVVSVVRLFQKERTTNVLVRDAASQPPRLGMFTTNALQRAILSGRPLEQIAVGELSN
                     YALISVRPSDLVGDALATMVRHHIHRVVVREGEQVLGVLEALDVFSFLSNHSMLITLR
                     INGARDIDELAEAAAQITDMIRRQFRGGTRAELIARLVQDLNARLFDRAWQLIAPAEL
                     VAHSCLFVMGSEGRGEQLLKTDQDNGLILRDGYAPPADLAELCQRFSDALARFGYPPC
                     PGGIMVSNPAWRMGATEFAQTTRRWLALPEGDSLMNLAIFMDAHAVSGDASLLDQVRD
                     SLTQMAVDNDAMLARFAGAIDAFGGSIGWWNRLLGGEAAQLLNLKKEGIFPLVHGVRS
                     LALAARLPVTGTVARIEALVQRGELSADTGRELTDSLHFFLSLRLKAGLAELDRQQPV
                     TGAVDFKSLNSLERDLLKDALDAVKRFKAQLRYRFRLNAL"
     gene            7233..7877
                     /locus_tag="PK242_09235"
     CDS             7233..7877
                     /locus_tag="PK242_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOZ94242.1"
                     /translation="MSGLFERLRRGWQRRQLTDPRWRFLLDEPPPHEWVALDCETTGL
                     DTRTDEIVAIGAVRIVGQRILTSERLELLVRPEKGVSAESIRVHRLRQQDVQTGVPVA
                     EAMQRLLHFIGARPLVGYYLEFDVAMINRALRATLGLTLPQEKIEVSRLYYDHKFRQL
                     PPYRQHDQADIDLRFATIMRELGLPTRDAHDALNDAVMAALAFIKLRVLRGDAD"
     gene            complement(7985..>8220)
                     /gene="actP"
                     /locus_tag="PK242_09240"
     CDS             complement(7985..>8220)
                     /gene="actP"
                     /locus_tag="PK242_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059048.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOZ94243.1"
                     /translation="LTVVSPAVWEATLGNPKGSAWFPYSSPALFSMTIGFVGVWLFSV
                     LDRSSNAAAERAAFAAQQVRSETGLGAAKASAH"
BASE COUNT         1224 a         2917 c         2929 g         1150 t
ORIGIN      
        1 tggagatgaa cacccgcctg caggtcgagc acccggtgac cgaggccatc accgggcagg
       61 acctggttga gtggcagctg cgcgtggcgg ccggcgagcc gctgccgctg gcgcaggacg
      121 aactgctgat cgacggccac gccatcgagg cgcgcatctg cgccgagaac cccgacaacc
      181 acttcctgcc cgccaccggc accctgcacg tgtaccgcaa gccgccgcac gcggccttcg
      241 agttcggcgc ggtgcgcttc gacgacggcg tgcgcgaggg cgatgccatc agccccttct
      301 acgacagcat gatcgccaag ctgatcgtct ggggcgaaac gcgccagcag gcgctggcca
      361 agctggacgc cgccctggcc gccacccaca tcgccggtct gcagaccaac gtgcagtttc
      421 tgcgccacgt gctggccagc gactcgttcg cccgggcccg actggacacc gccctgatcg
      481 agcgcgagcg cccccggctg ttccagcagg accggctggg tcgcgatctg gccgtggccg
      541 ccgccgtcgc ccagaccgtg ctggccgagc gcgcagccga aacccacgac cccttcagcc
      601 gcaccgacgg ctttcgctcg cacggcgtgg ccacgcgccg tgtcgatctg gaataccggg
      661 gcgaggcggt ggtggccaag ctgcgctacc ttcaggacgg cctgatgctg cagatcggcg
      721 acgccgaagc gcagccgctg tcgctcaccg cgctgccgga cggccgcatc gacctgcgct
      781 acggcaccgc acgcgagctg gtgcaggtgt acccgctggg cgaggtcgag catgtgttcg
      841 gcagccgtgg cgcggtgcag atcaccgagc tggacctgct ctcgcgcgcc gaagaaggtg
      901 ccggcgaagg cggccgcctg accgcgccca tgccgggcaa ggtggtgtcg atcgccgtcc
      961 gggccggcga caaggtcagc aagggccagg cgctggcggt gatggaggcc atgaagatgg
     1021 agcacaccat cgccgcgccg caggacggcg tggtggacga ggtgctgtac gcacccggcg
     1081 atcaggtcgc cgaaggggcc gagctgctgc gcctgagcca ccccggctga gcctcgggca
     1141 ggtcttggat tgctctatgt gtgatagctt tgcgcgcttg ccggatgcgc cccggaggtc
     1201 gatcaggcac tcagcgatgc gggcacggcg ccgagctgct cgaacggcat gctctgcttg
     1261 accaggatca ccgtgcaggg cgcgtccatg gcgaccttga tgggcaccgt ggcgacgaag
     1321 cgctgcagct gcagcccgtg cgtggccgcg cccatgacga tcatgcccac gtggttggcg
     1381 cgggcgaagc gcaggatcgc atcggccggg tcgccgtctt ccagcacgtg gtaggagatc
     1441 tggtgctgct cctgcggcag gccctgcgcc caggtcttga gcatggtcag catctggcgg
     1501 tgctgggtgg tttcgctgcg gctggcgtcg gtgctgctgg cggcgcccgg cgccaccacg
     1561 gtcaccaccg ccagccgcgc gccggggcgg atgcccagcg agcggcgcac cgcctcgcgc
     1621 agcgaataca gcgtggcgtc ggtgacgtcg tggtgcggca ccgccaccat caggatcggc
     1681 acctcgtcga tcacgcgcgt gggcagcggg ctgggctggt actgcgtgcc ggcggcgcgc
     1741 agccagcgct tgaagtgggt gcgaaagccg gtgcccttca ggcgccggcc gcgttcggtc
     1801 acctgcacct gctcgggctg ggcgaggtcg aacgccaggt gcgcggccga ggggtagcgc
     1861 tcggccgcca ccggctccag gcagcgcagg atcacttcct gcagccactc cggcaggtcg
     1921 ggccgccgcg cgcgcggcgg cttggggtcc atccacaggc gctggcgcag gccgcccttg
     1981 ctgcttggcg cgccaaacgg cagctcgccg gtggccagct cgtacagcat cacgccgatg
     2041 gcgaacacgt cgctgcgcgg atcgccgcgc acacccacca cctgttcggg cgacatgtag
     2101 gcgggcgagc ccacggcttc gcgcagctct tcggccagca ggtcggggta gtgggcgtgg
     2161 aacgacaggc cgaagtccag cagcaccgcg tgcagtgggg cgtcggcggg cgcgccctcg
     2221 gcggcgtcgc ggatgatgac gttggccggt ttcaggtcca ggtgcacggc gttctgctgg
     2281 tgcatgtcgt gcgccgcgcg cgccacggcg gcgcccaggc gggccagctc gtgcggcggc
     2341 agtggctgtc cctgcagcac ctggtccagc cagtggtcca gcgtgcggcc gggcacgtac
     2401 tccatcacca ggtagggcag caccgacagg tcacccgcgg ccaccaggta gggcacgtgc
     2461 gggccatcca gcgcctgcat catctggtgc tcgacctcga agctgacgat ggtctcggcg
     2521 ccgtcgccgg ccgtcatgcg cggcaccttc atcaccatcg ggaacggcgc cgcgcggccg
     2581 tcggcatagc gggtctcgta gatcagggcc atgccgccct tgtggatcat ctcgccgatc
     2641 agaaagccat cgatctcggc gcccggctcc agcgcgctca tcggccctgc tccagccggt
     2701 cggcaaagaa gggtggcagc ggcgtggcgc ggatcgcccc cgcggcggac ggtacgtcgt
     2761 agggcacgcg atggaaggtg agctgctgcg ccacgtcgtc gaacagcgcg tacatggcgt
     2821 cggtgcggcc gtcgcgcggc tggccgaccg agcccaccgt ggccagccag cgccggtggc
     2881 gcggcaccgg caccggcacg cctgcggtcg gggtgaacgg catcagcttg ccggtgccgc
     2941 cgcggtagta cagcgtctgc cggtgcacat ggccgccgaa cacgtagtgc acgtcgggcc
     3001 cgcccaccgg gggttcgcag gcggcgtcca ggctgagcgc cgccagcagc ggcgtctcga
     3061 cgtagtgcca gcgctccggc gcggcggcgc tggcgtgcac cagcagcagc ggcggctggt
     3121 gcagggtgag cggcagctcg gccagaaagc gccgctgcgc cgcactgagc tgctcctgcg
     3181 tccacagcgc ccccgcctgg cccagcgtct tgccgtcgcc cgctgcgtcg gtggcgtaca
     3241 cgtcgtggtt gcccttgacc accagcgcgc cttcggcggc caggtccatc acccgctgca
     3301 ccacgtaggc cgggtcgccg ccatagccga ccagatcgcc cagaaaggcc aggcgctcgg
     3361 cgccggcggc ctgcgcatgg gccaggcagg cgtccagcgc ctggcggttg gcatggaggt
     3421 cggaaagcag ggcaagccgc atgagaatcg atccggtggt cgggcggtgg gctggggaat
     3481 ttcacatcat acggggcggc aggcgcccgc ccgccgccgc taaactgacc accgacatgc
     3541 ctgccaccgc cggagaatcc ccatgagcct gcccacccaa gtccgcctga tcgacgttgg
     3601 cccgcgcgac ggcctgcaga acgaaaagca gccggtgccg gcggcggtca agatcgaact
     3661 ggtgcagcgc ctgcaggccg ccggtctgcg cgagatcgag gtcaccagct acgtcagccc
     3721 caagtgggtg ccgcagatgg ccgacaacca ccaggtgatg ggcggcatcg cgcgccagcc
     3781 cggcgtgcgc tacagcgtgc tgacccccaa cctgaagggc ttcgaggcgg cgctggccga
     3841 ccggccgcac gagatcgtgg tgttcggtgc cgccagcgag gccttcagcc agaagaacat
     3901 caactgctcg gtggccgaga gcatcgagcg ctttgccccg gtggtggcgg ccgcccgggc
     3961 cgccggcatc gccgtgcgcg gcgccatgag ctgcatcgtc ggctgcccct acgaaggcga
     4021 catcgcgccc gagaaggtgc gccagctggc cgggttgatg aagggcatcg gcgtcgaacg
     4081 cgtcgacgtg gccgacacca tcggcgtcgg cacgccgcgc aaggtgcagc gcgccatcga
     4141 ggccacgctg gcgcattacg acatcgacca cgtcagcggc catttccatg acacctacgg
     4201 gcaggcgctg gccaacaccc tggcggcgct ggagctgggg gtgtggaact tccagtcctc
     4261 ggtggccggc ctgggcggct gcccctacgc caggggcgcc accggcaacg tcgccagcga
     4321 ggacctggtg ttcatgctgc acggcatgga catcgccacc ggcatcgacc tcgaccgcct
     4381 gatcgacgcc ggccagttca tcagccagca cctggggcgc ccgcccaact cgcgcgtggc
     4441 cacggcggtg ctgaacaagc gcgccggctg acgggcgccc gggcgccgcg gctaccatcg
     4501 acctcatgaa ccaccccacc tctcccgccc tgcccgacgc catgcggcgc gtggccgccg
     4561 tgctggcggc gcacggccac ccccacgccc cgatcctgct cgacgacgcc gcgcgcaccg
     4621 cgcagcaggc ggccgatgcg ctgggggtgg tggtggggca gatcgccaag agcatcatct
     4681 ttcgccgccg cagcgacgac gcggcggtgc tggtgatcgc cgccggcgac aggcgggtgg
     4741 acgagaaaaa ggtggcggcg ctggtcggcc cggtgggccg cgccgacgcc gacttcgtca
     4801 aggcgcgcac cgggttcacc atcggcggcg tgtcgccggt ggcccacgcc agcccgccgg
     4861 tggtgctgat cgaccgcgag ctgtaccgct tcgacgtcat ctgggccgcc gccgggcacc
     4921 cgcacggcgt gttccgcctg gcgccggccg atctggagcg gctcaccggc gcaccggtgg
     4981 ccgatgtggc ccagggctga caggccgtgg cgccggtgcc gcgcgacttc gacaccctgg
     5041 cgcgacaggc gcagcaggcc ctgcgcgacg gtacgccgct gccgtccccc tgtatcggcg
     5101 tgtgccgcat cggcgatgcc ggtgtgtgcg tcggctgcgc ccggcggctg gacgagatcg
     5161 gcggctggct gcacgccgac gaggccgggc gcggggccat ctggcggcgc gtgctgcagc
     5221 gccgtggcct gcgggccggc gccggcggtg cggcgcccga ccctgcaagg ctggcgcaag
     5281 gcgagcagcg ctgaataggc tctgcggctt cccgcacgct atgaataacg cagctgctcg
     5341 cgctgagtgc gcgggcgctg taagcccatt tgattcagca ttcgaggggc cgacccggcc
     5401 cgcgccatgc ccaacgcctt caacttcaac gcctcgccgt tcgacagcct gacgccgcag
     5461 gagcagcgcc tggtgcgcga ccatgtggac gtggtctatt tccgacccga cgaggtgatc
     5521 ctcgacgccg gcatcgcccc catgcacctg ttcgtcatca tcaagggcgt ggtgcagcag
     5581 ttcgacggcg aggagctggt ggccacctac gggccggacg acagcttcga cggtcgcgcg
     5641 ctggtggcgg gccgcgccag cagccgcttc gtggcggtcg aggaggtggt ggcctatgcg
     5701 ctggcacgcc agaccgtcaa cgacctgatc gccgccaacg ccaccttcgg cgcgctggtg
     5761 ttttccgatc tgggggccaa gctcagcgcc gccagccagc gccacagcgg caacgagctg
     5821 caggcgctca acatggcgcg cgtggacgag gccttcgtgc gcccggcgca cctggtggag
     5881 gccgacaccg acgtggtgtc cgtggtgcgc ctgttccaga aggagcgcac caccaacgtg
     5941 ctggtgcgcg acgccgccag ccagccgccg cgcctgggca tgttcaccac caatgcgctg
     6001 cagcgcgcca tcctgtcggg ccggccgctg gagcagatcg cggtcggcga gctgagcaac
     6061 tacgcgctca tcagcgtgcg cccgtccgac ctggtgggtg acgcgctggc caccatggtg
     6121 cgccaccaca tccaccgtgt ggtggtgcgc gagggcgagc aggtgctggg cgtgctggag
     6181 gcgctggatg tgttcagctt cctgtccaac cactcgatgc tgatcacgct gcgcatcaac
     6241 ggcgcgcgcg acatcgacga gctggccgag gccgcggcgc agatcaccga catgatccgg
     6301 cgccagttcc gcggcggcac gcgcgccgag ctgatcgcgc gcctggtgca ggacctcaac
     6361 gcacgcctgt tcgaccgcgc ctggcagctc atcgcgccgg ccgaactggt ggcccacagc
     6421 tgcctgttcg tcatgggcag cgaaggccgc ggcgaacaac tgctcaagac cgatcaggac
     6481 aacggcctga tcctgcgcga cggctacgcg ccgccggccg atctggccga gctctgccag
     6541 cggttttcgg acgcgctggc gcgctttggc tacccgccct gcccgggcgg catcatggtc
     6601 agcaacccgg cctggcgcat gggcgcgacc gaatttgccc agaccacgcg gcgctggctg
     6661 gccctgcccg agggcgacag cctgatgaac ctggccatct tcatggacgc gcacgcggtc
     6721 agcggcgacg ccagcttgct ggaccaggtg cgcgacagcc tgacgcagat ggcggtcgac
     6781 aacgacgcca tgctggcgcg ctttgccggc gccatcgacg ccttcggcgg cagcatcggc
     6841 tggtggaacc gcctgctggg cggcgaagcg gcgcagctgc tgaacctcaa gaaggaaggc
     6901 atcttccccc tggtgcacgg cgtgcgcagc ctggcgctgg cggcgcgcct gccggtcacc
     6961 ggcacggtgg cgcgcatcga ggcgctggtg cagcgcggcg aactgagcgc cgacaccggg
     7021 cgcgagctga ccgacagcct gcacttcttt ctcagcctgc gcctgaaggc cgggttggcc
     7081 gagctcgacc gccagcagcc ggtgacgggg gcggtggact tcaagagcct caacagcctg
     7141 gagcgcgatc tgctgaagga cgcgctcgat gcggtcaagc gcttcaaggc gcagctgcgc
     7201 taccgcttca ggctcaacgc cctgtgacgg ccatgagcgg cctgtttgaa cgtttgcggc
     7261 gcggctggca acgccgccag ctcaccgacc cgcggtggcg cttcctgctg gacgagccac
     7321 cgccgcacga atgggtggcg ctggactgcg agaccaccgg gctggacacc cgcaccgacg
     7381 agatcgtcgc catcggcgcc gtgcgcatcg tcggccagcg catcctgacc agcgagcggc
     7441 tggagctgct ggtgcggccc gagaagggcg tgtcggccga aagcatccgc gtgcaccgcc
     7501 tgcgccagca ggacgtgcaa accggcgtgc cggtggccga ggccatgcag cgcctgctgc
     7561 acttcatcgg cgcacggccg ctggtgggct attacctgga gttcgacgtc gccatgatca
     7621 accgcgcgct cagggccacc ctgggcctga cgctgccgca ggaaaagatc gaggtctcgc
     7681 gcctgtacta cgaccacaag ttccgtcagc tgccgcccta ccggcagcac gaccaggccg
     7741 acatcgacct gcgctttgcc accatcatgc gcgagctggg cctgcccacg cgcgacgccc
     7801 acgacgccct gaacgacgcc gtgatggccg ctctggcctt catcaagctg cgcgtgctgc
     7861 gcggtgacgc cgactgagct tcaaaatcca tagctgctgg cgcttgtcct gattgcgcca
     7921 gaggccgata gggcacaaaa aaagaccggg tccgaagacc cggtttccgt ggccgaaggc
     7981 agggtcagtg cgccgacgcc ttggctgcgc ccaggccggt ttcggagcgc acctgctggg
     8041 cagcgaaggc cgcgcgctcg gcggcggcgt tgctgctgcg gtcgagcacc gagaacagcc
     8101 acacgccaac aaagccgatg gtcatcgaga acagcgccgg cgacgagtag gggaaccagg
     8161 cgctgccctt ggggttgccc agcgtggctt cccacaccgc gggcgacacc accgtcagca
//
LOCUS       DAOOOG010000117         8169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_10305_length_8169_cov_27.132980, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000117 DAOOOG010000000
VERSION     DAOOOG010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10305_length_8169_cov_27.132980"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PK242_09245"
     CDS             complement(<1..641)
                     /locus_tag="PK242_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOZ94244.1"
                     /translation="MSAVLPTPHAGHDDHAHDHHHAPTGWRRWVYATNHKDIGTLYML
                     FSFFMLMVGGVLALLIRAELFQPGLQVVNPELFNQLTTMHGLIMVFGAIMPAFVGFAN
                     WMIPLQIGASDMAFARMNNFSFWLLIPAAIMLVGSFFMPGGAPAAGWTLYAPLTLQMG
                     MSMDSSILAMHIMGASSIMGAINIIVTILNMRAPGLTLMKMPMFCWTWLITAYL"
     gene            complement(700..1884)
                     /gene="coxB"
                     /locus_tag="PK242_09250"
     CDS             complement(700..1884)
                     /gene="coxB"
                     /locus_tag="PK242_09250"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOZ94245.1"
                     /translation="MASKLTGRASQVALWGALWLAGAAQAQVNDLPGGPAVRQLNLHP
                     PVTKIAEAQHSLHWMLLIVCTIIFIGVFGVMFYSIWKHRKSQGAKPAQFHESVAIEVT
                     WTVVPFLIVIGMALPATKVVVAQKDTTNADLTIKATGYQWKWGYDYLNGEGAGIGFLS
                     TLDSSHRAMSDAGKPAGDDYLLKVDHPLVVPVGKKVRIITTANDVIHSWMVPAFGVKQ
                     DAIPGFVRDTWFRAEQTGDFYGQCAELCGKEHAYMPIHVKVLPQHEYTAWVEQERKRL
                     AALADDPNKSWTLAELVARGEKVYAANCAACHLENGRGSAAIKPLDGSAIVKGPAAEQ
                     MRVVLEGRANGTMPAWKQLSDVELAAVVTYTRNHWTNQTGTLVQPAEFKAARTGKFPA
                     GG"
     gene            complement(1941..2429)
                     /locus_tag="PK242_09255"
     CDS             complement(1941..2429)
                     /locus_tag="PK242_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HOZ94246.1"
                     /translation="MQQASTQRWAHVGDQALAWRLRRHCSVTPRQLAGLYAAMCLLSL
                     SIAVGFWALGMPLVLAFAGLELLAVGAALLVHARHALDGETVHLQGGRLIVERECAGR
                     CERLEFGAAQVRIAPPASERGLIELSAAGRRIAIGRHVRPSQRPALASDLRKALWAGG
                     LH"
     gene            complement(2520..3443)
                     /locus_tag="PK242_09260"
     CDS             complement(2520..3443)
                     /locus_tag="PK242_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HOZ94247.1"
                     /translation="MSPAASDRPPSIDLVAARRWAERQHHGSPWLHEEVARRMAERLA
                     VIRLPVRAWAHWEPVRGGLQGHERVAAQYPDAECFRVSSQYPSAPEATKNIASGWWQR
                     LTGGRPSAHDVARPPEASVQLVWANMLAHQVAEPAALMADWLRALAVDGFVMFSCLGP
                     DTLRELRALYADLGWPPPAHEFTDLHDWGDQLLAAGFADPVMDMERITLSFATPQRLL
                     QELRGLGRNLHVQRAAGLRTPAWRAQLQAALARHPALQAGDGALTLSFEVIYGHAVKP
                     PPRHTIAPQTRISLDDLKVSLRQRNRNLPSD"
     gene            3558..4289
                     /locus_tag="PK242_09265"
     CDS             3558..4289
                     /locus_tag="PK242_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOZ94248.1"
                     /translation="MRPMTLLRAWLRHPAGASAWPSQCLICRRWPAQTLCAECRARFA
                     PQLTRCQRCALAVPAGVAVCGACLREPPPMAQCLAAVSWQWPWSMCIGRWKFEGDTGL
                     SGPLAALLQATPGVAEALAAAELLLPMPMSDQRLAERGYNPALLLARRLAPGRVQPGW
                     LLRTRHTPPQRGLPRAERLRNVRGAFQVDPLAAGRLRGRRVLLVDDVMTTGASVREAA
                     ATLRAAGADPVGVLVLARTDEPTTH"
     gene            4312..4791
                     /gene="trmL"
                     /locus_tag="PK242_09270"
     CDS             4312..4791
                     /gene="trmL"
                     /locus_tag="PK242_09270"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HOZ94249.1"
                     /translation="MFHIVLVEPEIPPNTGNVIRLAANTGCTLHLIEPLGFSMDDRQL
                     RRAGLDYHEYAPVRRHAGWEAFVASERPAAERLFALTTRGTRGVHEVSFAAGDWLVFG
                     AETRGLPAALRERLPAAQQLRLPMRPGQRSLNLSNAVAVTVFEAWRQNGFGGAGAPV"
     gene            complement(4935..5117)
                     /locus_tag="PK242_09275"
     CDS             complement(4935..5117)
                     /locus_tag="PK242_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94250.1"
                     /translation="MPTDAVARMSMRCHTPNAGASLNGPLTTPAGVVTTAALAAPCSD
                     RQRAGQEGAPNPGSAG"
     gene            5264..5794
                     /locus_tag="PK242_09280"
     CDS             5264..5794
                     /locus_tag="PK242_09280"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HOZ94251.1"
                     /translation="MRPAPSPAHFHPVFRIRVDNDGNPATTADQSWLVFERGAMGPYT
                     APTGTWVTETLGDSTLMWVWQSGAANSQPPVTTMAQFKAGSYVPESGGFTIPATATIL
                     GVQVGTGSGWYGVFRDAVDTIGLAAQSSLGPDNFELTAPPPAAVAPVPTLDGLGLSAL
                     AGLLAGLGLWRQRRRG"
     gene            complement(6071..7405)
                     /locus_tag="PK242_09285"
     CDS             complement(6071..7405)
                     /locus_tag="PK242_09285"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOZ94252.1"
                     /translation="MKSLVGALYDLATWLAQPLLRRKLRRRGAAEPGYLHAVEERFGH
                     YTQARPVGAAPLVWVHAVSLGETRAAAILLAALRPRLPGMRLLLTHGTATGRAEGGRL
                     LQAGDVQVWQPWDSPGAVRRFLAHWRPDVGVLMETEIWPNLIREAAARGVPLALANAR
                     LSDRSLRGARRAGALLRPAYRALSAVWAQTEADAARLRALGAGPVTVLGNLKFDAQPA
                     PELLARGRAWRAGSDRPVVMLASSRDGEEAEFLKQIKALELSGQAPEAIENVATEAAA
                     PAVQWLIVPRHPQRFDDVAAAVAAAGLSLSRRSAWHALPEAAEVWLGDSLGEMAAYYA
                     LADVALLGGSFAPLGGQNLIEAAACGCPVLMGPHTFNFAQAAELAQAAGAARRVADLA
                     QAVHAARALLADAPARAAMRAAAQDFAQAHRGAAARSAEALQALLTGAEPAA"
     gene            complement(7470..>8169)
                     /locus_tag="PK242_09290"
     CDS             complement(7470..>8169)
                     /locus_tag="PK242_09290"
                     /EC_number="5.4.2.2"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805264.1"
                     /note="catalyzes the interconversion of alpha-D-mannose
                     1-phosphate to alpha-D-mannose 6-phosphate and
                     alpha-D-glucose 1-phosphate to alpha-D-glucose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOZ94253.1"
                     /translation="GQNIFPDRQLVLFAQDVLTRVPGAPIVFDVKCSQRLAPAIRAAG
                     GVPLMHQTGHSLIKARMKEVGAPLGGEMSGHIFFQERWYGFDDGTYAGCRLLEILSRS
                     PDASAVLNALPTSCSTPELNVPCAEGEAHRLAAELQALAPVVFAEPLSARPEGSEPRA
                     AGSAGAAAISTIDGLRVDWPDGFGLIRASNTTPVLVLRFEGQTPQALARIQADMLALL
                     RRVKPDAQLGAAAH"
BASE COUNT         1172 a         2937 c         2861 g         1199 t
ORIGIN      
        1 aggtaggcgg tgatcagcca ggtccagcag aacatgggca tcttcatcag cgtcaggccg
       61 ggcgcgcgca tgttcaggat ggtgacgatg atgttgatgg cgcccatgat ggagctggcg
      121 cccatgatgt gcatggccag gatgctgctg tccatcgaca tgcccatctg cagcgtcagc
      181 ggcgcgtaca gcgtccagcc ggcggccggc gcaccgcccg gcatgaagaa cgagccgacc
      241 agcatgatgg cggccggaat cagcagccag aagctgaagt tgttcatgcg cgcgaaggcc
      301 atgtcgctgg cgccgatctg cagcgggatc atccagttgg cgaagcccac gaaggccggc
      361 atgatggcgc cgaacaccat gatcagcccg tgcatggtgg tcagctggtt gaacagctcg
      421 gggttgacca cctgcaggcc gggctggaac agctcggcgc ggatcagcag cgccagcacc
      481 ccaccgacca tcagcatgaa gaaggaaaac agcatgtaca gcgtgccgat gtccttgtgg
      541 ttggtggcgt acacccagcg gcgccagccg gtgggcgcgt ggtggtggtc gtgggcgtgg
      601 tcgtcgtgac cggcgtgggg ggtgggcagt acggcgctca tggcaatctt tccgggattc
      661 tgaaacttgg acgggcgggt cagggttcag cgcggacgct caacctccgg cggggaactt
      721 gccggtgcgc gccgccttga attcggccgg ctgcaccagc gtgcccgtct ggttggtcca
      781 gtggttgcgc gtgtaggtga cgaccgccgc cagctcgacg tcggacagct gcttccaggc
      841 cggcatggtg ccgttggcgc gcccctccag caccacccgc atctgctcgg ccgccggacc
      901 cttgacgatg gcgctgccgt ccagcggctt gatggcggcg ctgccacggc cgttttccag
      961 gtggcaggcg gcgcagttgg cggcatacac cttttcgccg cgcgccacca gctcggccag
     1021 cgtccacgac ttgttggggt cgtcggccag cgccgccagg cgcttgcgtt cctgctcgac
     1081 ccaggcggtg tattcgtgct gcggcagcac cttgacgtgg atcggcatgt aggcgtgctc
     1141 cttgccgcac agctcggcgc actgaccata gaagtcgccg gtctgctcgg cgcggaacca
     1201 ggtgtcgcgc acgaagccgg ggatggcgtc ctgcttgacg ccgaaggccg gcaccatcca
     1261 ggagtggatg acgtcgttgg cggtggtgat gatgcgcacc ttcttgccga ccggcaccac
     1321 cagcgggtgg tcgaccttca gcaggtagtc gtcgccggcc ggcttgccgg cgtcggacat
     1381 ggcgcggtgc gaggagtcca gcgtggacag gaagccgatg cccgcgccct cgccgttcag
     1441 gtagtcgtag ccccacttcc actggtagcc ggtggccttg atggtgaggt cggcgttggt
     1501 ggtgtccttc tgtgccacca ccaccttggt ggccggcagc gccatgccga tcacgatcag
     1561 gaacggaacc accgtccagg tcacctcgat cgccaccgac tcgtggaact gggccggctt
     1621 ggcgccctgc gacttgcggt gcttccagat ggaatagaac atcacgccga acacgccgat
     1681 gaagatgatc gtgcagacga tcagcagcat ccagtgcagg ctgtgctggg cctcggcaat
     1741 cttggtcacc ggcgggtgca ggttgagctg ccgcacggcc gggccacccg gcaggtcgtt
     1801 gacctgcgcc tgcgcggcgc cggccagcca caacgcgccc cacagggcca cctgggacgc
     1861 ccttccagtc agcttgcttg ccatgcgatt catcgtgccc acgttgttca ctgcccccac
     1921 catagattca aatgtcccac tcaatgcagc ccaccggccc acagcgcctt gcgcaggtcg
     1981 ctggccagcg ccggacgctg cgacggccgc acatggcgac cgatggcgat gcgccggccc
     2041 gccgccgaca gctcgatcaa gccccgctcg ctggcgggcg gcgcgatgcg cacctgcgca
     2101 gcgccgaatt ccagccgttc gcaccgaccg gcgcattcgc gctcgacgat cagccgaccg
     2161 ccctgcaggt gcaccgtttc accgtccagc gcatgccgcg catgcaccag cagcgcggcg
     2221 ccgacggcca gcagctccag cccggcaaac gccagcacca gcggcatgcc cagcgcccag
     2281 aagccgactg caatcgacag cgacagcagg cacattgcgg cgtacagccc agccaactgg
     2341 cgcggcgtca cgctgcagtg gcgccgcagc cgccaggcca gggcctggtc accgacgtga
     2401 gcccatcgct gggtcgaagc ttgctgcaag acgaggttct ccgcatcacc gcaaagcgcc
     2461 gtcgcttcgc tatgaatgca aatgagttta accgagtcgc cgcaggtctt tgataaacct
     2521 caatcagagg gcaaattcct attgcgctgt cgcaatgaaa cctttaagtc gtccagcgaa
     2581 atgcgggtct ggggcgcgat ggtgtggcgt ggcggtggct tgacggcatg gccatagatg
     2641 acctcgaaac tcagcgtcag ggcaccgtcg cctgcctgca gcgccgggtg gcgcgccagc
     2701 gccgcctgca gctgcgcccg ccaggccggc gtgcgcaggc cggccgcgcg ctgcacatgc
     2761 aggttgcgcc ccaggccgcg cagctcctgc agcagccgct gcggggtggc aaagctgagc
     2821 gtgatgcgct ccatgtccat caccggatcg gcaaagccgg ccgccagcag ctgatcgccc
     2881 cagtcgtgca ggtcggtgaa ttcgtgcgcc ggcggcggcc agccgaggtc ggcgtacagc
     2941 gcacgcagct cgcgcagcgt gtcgggcccc agacaggaga acatgacgaa gccgtcgacc
     3001 gccagcgcgc gcagccagtc ggccatcagc gcggccggct cggccacctg gtgcgccagc
     3061 atgttggccc acaccagctg caccgacgcc tccggcggcc gggccacgtc atgcgccgac
     3121 gggcggccgc cggtgagccg ctgccaccag ccggatgcta tatttttggt agcttctggc
     3181 gctgacgggt attgacttga gaccctgaaa cactcggcat ccggatactg cgccgcgacc
     3241 cgctcatgcc cctgcaggcc accgcgcacc ggctcccagt gcgcccaggc gcgcaccggc
     3301 aggcggatca cggccagccg ctcggccatg cgccgcgcca cttcttcgtg cagccagggc
     3361 gagccgtggt gctgccgttc ggcccagcgg cgcgccgcca cgaggtcgat gctgggaggt
     3421 cggtcagagg ccgcaggcga catggccggc cagtatattg atgcgctccc gcgcaaaaag
     3481 atggcccatg gcgcccgggg tcatcgccgc gccgcccggt cattcgtcat tcccaattcg
     3541 tttacaagcc cccgcccatg cgccccatga ccctgctgcg tgcctggctg cgccaccccg
     3601 ccggcgcttc ggcctggccc agccagtgcc tgatctgccg ccgctggccg gcgcagacgc
     3661 tgtgcgccga atgccgcgcg cgctttgctc cgcagctcac gcgctgccag cgctgcgcgc
     3721 tggcggtgcc ggcgggcgtg gcggtgtgcg gcgcctgtct gcgcgagccg ccgccgatgg
     3781 cccagtgcct ggccgccgtg tcctggcaat ggccgtggtc gatgtgcatc gggcgctgga
     3841 agttcgaagg cgacaccggc ctgagcggcc cgctggcggc gctgctgcag gccacgccgg
     3901 gggtagccga ggcgctcgcc gccgccgagc tgctgctgcc gatgccgatg tcggaccagc
     3961 gcctggccga gcgcggctac aaccccgccc tgctgctggc gcgccgcctg gcgcccgggc
     4021 gggtgcagcc ggggtggctg ctgcgcacgc gccacacgcc gccgcagcgc ggcctgccgc
     4081 gcgccgaacg cctgcgcaac gtgcgcgggg ccttccaggt cgatccgctg gccgccgggc
     4141 gcctgcgcgg ccgccgcgtg ctgctggtgg acgacgtcat gaccaccggc gccagcgtgc
     4201 gcgaggccgc cgccacgctg cgcgcggccg gcgccgaccc ggtcggcgtg ctggtgctgg
     4261 cgcgcaccga cgagccgacc acgcactgaa ccttccacac agcctggcgc catgttccac
     4321 atcgttctgg tcgagcccga gattccgccc aacaccggca acgtgatccg gctggcggcc
     4381 aacaccggct gcacgctgca cctgatcgag ccgctgggct tttcgatgga cgaccgccag
     4441 ttgcgccgcg ccgggctcga ttaccacgaa tacgccccgg tgcgccgcca cgccggctgg
     4501 gaggcctttg tggccagcga acggcccgcc gccgagcgcc tgttcgccct caccacgcgc
     4561 ggcacgcgcg gcgtgcacga ggtcagcttt gccgccggcg actggctggt gttcggcgcc
     4621 gaaacgcgcg gcctgcccgc ggcgctgcgc gagcgcctgc cggcggcgca gcagctgcgc
     4681 ctgccgatgc ggcccggcca gcgcagcctg aacctgtcca acgcggtggc ggtcacggtg
     4741 ttcgaggcct ggcggcaaaa cggctttggc ggggccggtg cgcctgtctg aaccacgcat
     4801 gggctgcgcg ggctgtgttg ctttttgtta caagtacgcc ggtatctgcg ccaccaacgc
     4861 cgttggtccc gatcccgtcc gcgacaatcg cggctgatcg ttcaccacag tgaagggtgc
     4921 ccatgaaccc cacatcaacc tgccgaaccc ggattcggcg caccctcctg gccagcgcgc
     4981 tggcggtcgc tgcagggggc ggccagcgcg gccgtcgtca ccacgccggc aggcgtcgtc
     5041 aacgggccat tcagcgatgc gccggcgttc ggtgtgtggc agcgcatcga cattcgcgcc
     5101 accgcgtcgg tcggcatcac gggcgactac ccgcgcaatg gcgatggctc cgtgctgatg
     5161 acctccggcg acggcaccgg caagaccacc tggcagcact ttcccgcggg tggcctgggc
     5221 ccgctgagca gcttcatttc ggccacctac gagtggtacc gcgatgcggc cagcaccgtc
     5281 acccgcccac tttcatccgg tgttccgcat tcgggtggac aacgacggca accccgccac
     5341 cacggcagac cagtcctggc tggtctttga gcgcggcgcc atgggcccct acaccgcgcc
     5401 taccggcacc tgggtgaccg aaaccctcgg tgacagcacc ctgatgtggg tctggcagag
     5461 cggcgccgcc aacagtcagc cccctgtcac gaccatggcg caattcaagg ccggcagcta
     5521 cgtccccgag tcgggaggtt tcaccatccc agcgaccgcc accatcctgg gcgttcaggt
     5581 tggcaccggt tcgggctggt atggcgtgtt ccgagacgcg gtggacacca ttggcctcgc
     5641 cgcccaatcc agcctggggc ccgacaactt cgaactgacg gcaccgccac cggccgccgt
     5701 tgcccctgtg cccaccctcg acgggctggg cctgagcgcg ctggccggcc tgctggcggg
     5761 tctggggctg tggcgccagc gccggcgcgg ctgatcctag aaacggcagt tgaccgcttg
     5821 tcaacttcgg gcaggccgca tcgatgcggg gactcgcggc tgcgatggcc ttcacctcct
     5881 gggtgagcac gctacgcacc cacccgcacc gcgctcggcg cgccatgcgg cgttgctcct
     5941 ccttgcgggc aggagcccgc ggcgtcgtcg cgtcttgcct ggcacgccga tcacggcacg
     6001 ggcgggggcg tccaactgcc gtttctaggc tgatcggcgc caacgcccgg gcgcgcggcg
     6061 gtggccggcg tcacgcggcc ggttcggcgc ccgtcagcag cgcctgcagc gcctcggccg
     6121 accgcgctgc cgcaccgcgg tgggcctggg caaagtcctg ggccgccgcg cgcatggccg
     6181 cgcgggccgg cgcatcggcc agcagggcgc gcgcggcgtg caccgcctgg gccaggtcgg
     6241 ccacgcgccg cgccgcaccc gcggcctggg ccagttcggc ggcctgggca aagttgaagg
     6301 tgtgcggccc catcagcacc gggcagccgc aggcggccgc ttcgatcagg ttctgcccgc
     6361 ccagcggggc aaagctgccg cccagcaggg ccacgtcggc cagggcgtag tacgcggcca
     6421 tttcgcccag gctgtcgccc agccagacct cggccgcctc gggcagcgcg tgccaggcgc
     6481 tgcgccggct caggctcagg ccggcggcag cgacggcggc ggccacgtcg tcgaatcgct
     6541 gcggatggcg cggcacgatg agccattgca cggccggcgc cgccgcctcg gttgctacgt
     6601 tttcaatagc ttccggcgct tgcccagaaa gctccagggc cttgatttgc ttgagaaact
     6661 cagcctcctc gccgtcgcgc gagctggcca gcatgaccac cggccggtcg ctgccggcac
     6721 gccaggcgcg gccgcgcgcc agcagttcgg gcgccggctg ggcgtcgaac ttcaggttgc
     6781 ccagcacggt gaccgggccg gcgccgaggg cgcgcaggcg tgccgcgtcg gcctcggtct
     6841 gcgcccacac cgccgacagc gcgcggtagg ccgggcgcag cagcgcgccg gcgcgccggg
     6901 cgccgcgcag cgaacggtcg gacaggcgcg cgttggccag cgccagcggc acgccgcgcg
     6961 ccgcggcctc gcggatcagg ttgggccaga tctcggtttc catcagcacg ccgacgtcgg
     7021 gccgccagtg cgccagaaag cgccgcacgg cgccgggcga gtcccagggc tgccagacct
     7081 gcacgtcgcc ggcctgcagc agcctgcctc cctcggcgcg tccggtggcg gtgccatgcg
     7141 tgagcagcag gcgcatgccg ggcaggcgtg ggcgcagcgc cgccagcaga atggcggcag
     7201 cgcgcgtctc gcccaacgac accgcgtgca cccacaccag cggcgcggcg cccaccgggc
     7261 gggcctgggt gtagtggccg aagcgctcct ccacggcatg caggtagccc ggctcggcgg
     7321 cgccgcggcg gcgcagcttg cgtcgcagca ggggctgggc cagccaggtg gccaggtcgt
     7381 agagggcgcc gacgagcgac ttcatgcgct atggtttgtg tagcttctgg cgcacgccgg
     7441 gtgggcgctg gggccgtcaa acgcgcccat caatgggcag cggcgccgag ctgggcgtcg
     7501 ggcttgacgc gccgcagcag cgccagcatg tcggcctgga tgcgcgccag cgcctgcggc
     7561 gtctggccct cgaagcgcag caccagcacc ggcgtggtgt tgctggcgcg gatcaggccg
     7621 aagccgtccg gccagtcgac gcgcaggccg tcgatggtcg agatcgcagc cgcccccgcg
     7681 ctgcccgctg cgcgtggttc gctgccctcc gggcgggcgc tcaggggctc ggcaaacacc
     7741 accggcgcca gcgcctgcag ctcggccgcc agacggtgcg cctcgccttc ggcgcagggc
     7801 acgttcagct cgggcgtgct gcaactggtg ggcagggcat tgagcacggc gctggcgtcg
     7861 ggcgagcggc tgaggatttc cagcagccgg cagccggcgt aggtgccgtc gtcgaagccg
     7921 taccagcgct cctgaaagaa gatgtggccg ctcatttcgc cgcccagagg cgcgccgacc
     7981 tccttcatgc gcgccttgat cagcgaatgg ccggtctggt gcatcagcgg cacgccgccg
     8041 gcggcgcgga tggccggcgc caggcgctgc gagcacttga cgtcgaacac gatgggcgcg
     8101 cctggcacgc gggtcagcac gtcctgcgcg aacagcacca gctggcggtc ggggaagatg
     8161 ttctggccg
//
LOCUS       DAOOOG010000118         8129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_10376_length_8129_cov_29.194947, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000118 DAOOOG010000000
VERSION     DAOOOG010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10376_length_8129_cov_29.194947"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PK242_09295"
     CDS             complement(<1..537)
                     /locus_tag="PK242_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOZ94254.1"
                     /translation="MNPTDQFVGTRPVSEAHAFDSGALAAWMSAYVEGFAGPLGVEMF
                     KGGQSNPTYKLITPGRTYVMRAKPGPVAKLLPSAHAIEREFAVMRGLAGTDVPVARMH
                     ALCEDESVIGRAFYIMEFVEGRVLWDQTLPGMTPAERGAHYDELNRVIAALHKVDCAA
                     RGLADYGKPGNYFERQIGR"
     gene            complement(539..1258)
                     /locus_tag="PK242_09300"
     CDS             complement(539..1258)
                     /locus_tag="PK242_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ94255.1"
                     /translation="MDEPILTMEEREAINSGRWFSSLSPSLRHDILRCAFVKRYPDGE
                     LISARGEPPDLWVACAKGAVRVSSTTLTGKQITLTYVEPGVWFGDVAMFDGDQRTHDV
                     YAHGPTTLLCVARADFHKILAQHSELYEALLKLQARRIRLLFGQVEDLNTLPLRARLA
                     KQLAHLVRSYGVPSLSSNDEIRIALQLAQEELAQLLGASRQRVNQELKTMEREGVIRV
                     EPTGLVVCDRDALLRIGEGEQ"
     gene            complement(1343..2122)
                     /locus_tag="PK242_09305"
     CDS             complement(1343..2122)
                     /locus_tag="PK242_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOZ94256.1"
                     /translation="MTAELRSTFEGSTMVLTISNPEHRNALGPEMYAAGVEALSAAES
                     NPEVRCVVLTGEGQVFCAGGNLQRLQANRQRPPEVQAQSIERLHDWVEAIRTFPKPVI
                     AAVEGPAAGAGFSLALACDLIVAARNAVFVMAYSSVGLSPDGGGSWSLARALPRQLVN
                     QLLMGGERIGAQRLHELGVVGRLCDAGQALGSALDWAGQLGARAPNVMASIKELVGEA
                     DRNDLSSHLALERDHFVRNLHHPNAGIGIAAFLAKQTPRYE"
     gene            complement(2418..3941)
                     /locus_tag="PK242_09310"
     CDS             complement(2418..3941)
                     /locus_tag="PK242_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOZ94257.1"
                     /translation="MNTPVFSRVGIVGTGAMGRGIAQIAAQAGSTVRLYDTQPDAVAK
                     AREALMAQWDKLVDKGRLTREQADSHVSRVPPAATLADLADCDLVIEAIVERLDVKQT
                     LFAELEGIVRPDAILATNTSSLSVTAIAAGLKHPGRLAGYHFFNPVPLMKVVEVIAGL
                     KTDAATGQRLTDYARQMGHTPVQAADTPGFIVNHAGRGYGTEALRIVGEGVTDFVTVD
                     RILRDQVGFRLGPFELFDLTALDVSHPVMESIYRQYFDEPRYRPSVITAQRLAGGVLG
                     RKSGEGFYRYLGGSAQVAPEPAAPQVAELPPVWVSTRAARRAELYQLLKDLGARIETG
                     QSPSPQALTLVAPLGFDVTTVAVVERLDPARTVGIDLLIEDAATRRRVLATNPATRSD
                     MRDAAHALFARDGKPVSVIHDSGGFVTQRVVAHIVNIAADMCQQRVCSPQDLETAVTL
                     GLGYPLGPLAMGDRWGPANMLEVLFNLQTVYGDPRYRPSPWLRRRGAIGLSLLHEEA"
     gene            complement(4075..5082)
                     /locus_tag="PK242_09315"
     CDS             complement(4075..5082)
                     /locus_tag="PK242_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HOZ94258.1"
                     /translation="MNIVILDDYQDAVRKLACASRLEPYPAKVYTNTVKGLGQLSVRL
                     RDAEVLVLIRERTQITRQLIEKLPRLKMISQTGRVGSHVDVAACTERGIAVAEGVGSP
                     VAPAELTWALIMAAMRRLPQYIGNLKHGAWQQSGLKSASMPPNFGLGQVLRGKTLGIW
                     GYGRIGQIVAGYGRAFGMQVLVWGSSDSMTRARLDGHQVAPSRAEFFALSDVLSLHLR
                     LVEATHAIVRLEDLSRMKPTALLVNTSRAELIEHDALVSALNRGRPGMAAVDVFESEP
                     ILQGHALLRLENCICTPHIGYVEQDSYELYFGAAFDNVVNYLRGTPTQIVNPGALQMR
                     R"
     gene            complement(5181..6347)
                     /locus_tag="PK242_09320"
     CDS             complement(5181..6347)
                     /locus_tag="PK242_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ94259.1"
                     /translation="MTIETTTDDSYLASAGRRQLLRAGAGSLSLLAAGGLFGGRVAVA
                     ATIDKPADVPVVDRVAIRVVTDSYHHAFEPPRTLGNLQVQRLGFDVKPGRPPRRTLQN
                     EWGLSLHIESGRGDEVRQVLLDFAYTPEALLNNLLLCGIDVSKLDAIALSHGHYDHFG
                     GMVGFLDAHKSKLKPGLPFYLGSEECFCSRELNVPGDAGSFGALDREAIRQSGMTMTF
                     AQRPSILASHGFTTGVIPSSSFERVLAPTRMTPGIQAGLGCAPEGLSPAKQSLTAAVP
                     DDFEHEQAIVYQLKGRGLVVMTSCGHRGIVNSVRRAMQVAGTDKVHAILGGFHLAPHT
                     PEYQRQTLAELKAINPDFLIPMHCSGETFISMAQSEMRDRFVRSSTGTRFVFSA"
     gene            complement(6507..6896)
                     /locus_tag="PK242_09325"
     CDS             complement(6507..6896)
                     /locus_tag="PK242_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94260.1"
                     /translation="MARFTTHQPTAATVLCLDDYRVPSYRRPRRPGSAGSSLIRPAPD
                     NAWSGLARQRALQTGAGGLPLHPDPAPTAAPCSAPPVRTLRLRDPQAPDAPARLRISG
                     RLIDVCAELERLAAAELANSPPQPRCA"
     gene            complement(7022..7687)
                     /gene="lexA"
                     /locus_tag="PK242_09330"
     CDS             complement(7022..7687)
                     /gene="lexA"
                     /locus_tag="PK242_09330"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOZ94261.1"
                     /translation="MSDMPKLTARQQQILQLVQHAIARTGAPPTRAEIAAELGFKSAN
                     AAEEHLQALARKGVIELVSGTSRGIRLKGAARSRLAGDAFSLTLPGMAQLALPLVGRV
                     AAGSPILAQEHVEQTYHLEPTLFTQRPDYLLRVRGMSMQGAGILDGDLLAVHATREAR
                     NGQIVVARLGDDVTVKRFHRHQHQIELHAENPDYPTIVVQPGEPFEIEGLAVGLIRNS
                     MLM"
BASE COUNT         1231 a         2664 c         2893 g         1341 t
ORIGIN      
        1 gcggccgatc tggcgctcga agtagttgcc gggcttgccg tagtcggcca ggccgcgcgc
       61 cgcgcagtcg accttgtgca gcgcggcaat gacgcggttg agctcgtcgt agtgggcgcc
      121 gcgctcggcc ggggtcatgc ccggcagtgt ctggtcccac agcacgcggc cttcgacgaa
      181 ctccatgatg tagaaggcgc ggccgatgac ggattcgtct tcgcacagcg cgtgcatgcg
      241 cgccaccggg acgtcggtcc cagccaggcc gcgcatgacg gcgaactcgc gctcgatggc
      301 gtgcgccgac ggcagcagct tggcgacggg gccgggcttg gcgcgcatca cgtaggtgcg
      361 cccgggcgtg atgagcttgt aggtggggtt ggactggccg cccttgaaca tctcgacgcc
      421 cagcgggccg gcaaagcctt cgacgtaggc gctcatccag gcggccaggg cgccgctgtc
      481 gaaggcgtgc gcctcggaca ccggacgtgt gccgacgaac tgatcggtgg ggttcatgtc
      541 attgttctcc ttcaccgatg cgcagcaggg cgtcgcggtc gcacaccacc aggccggtgg
      601 gctcgacgcg gatcacgccc tcgcgctcca tggtcttgag ctcctggttg acgcgctggc
      661 gcgaggcgcc cagcagctgc gccagctctt cctgcgccag ctgcagggcg atgcggattt
      721 cgtcgttgga cgacaggctg ggcacgccgt agctgcgcac caggtgcgcc agctgcttgg
      781 ccaggcgcgc gcgcagcggc agggtgttga ggtcttccac ctgtccgaac agcaggcgga
      841 tgcgccgcgc ctgcagcttg agcagggctt cgtacagctc ggagtgctgg gccaggatct
      901 tgtgaaagtc ggcgcgcgcc acgcacagca gggtggtggg gccgtgggca tagacgtcgt
      961 gcgtgcgctg gtcgccgtcg aacatggcca cgtcgccaaa ccacaccccc ggctccacgt
     1021 aggtcaaggt gatctgcttg cccgtcagcg tagtggagct gacacgcacc gcgcccttgg
     1081 cacaggccac ccacagatcg ggcggctcgc cacgggcgct gatcagctcg ccgtcggggt
     1141 agcgtttgac aaaggcgcat cgaaggatgt cgtgccgcag cgaaggggat agagaggaaa
     1201 accagcgacc actgttgatc gcctcgcgtt cctccatggt aagaatgggc tcgtccatgg
     1261 tctgtctttt gggtgactgc aagaggcggc attgtcgcgc cggggacggg gcccgccctg
     1321 gccgccggcc tgccgccggg aactactcgt agcgcggcgt ctgcttggcc aggaaggccg
     1381 cgatgccgat gccggcgttg gggtggtgca ggttgcgcac gaagtggtcg cgctccagcg
     1441 ccaggtggct ggacaggtcg ttgcgatctg cttcgccgac caattccttg atgctggcca
     1501 tcacgttggg ggcgcgggcg cccaattgcc cggcccagtc gagcgcactg cccagcgcct
     1561 ggccggcgtc gcacagccga ccgaccacgc ccagctcgtg caggcgctgc gcgccgatgc
     1621 gttcgccccc catcagcagc tggttgacca gctggcgcgg cagcgcgcgc gccaggctcc
     1681 agctgccacc gccgtcgggc gacaggccga cgctgctgta ggccatgacg aacaccgcgt
     1741 tgcgcgccgc cacgatcagg tcgcaggcca gcgcgagcga aaaaccggca ccggcggccg
     1801 ggccttcgac ggcggcgatg accggcttgg ggaaggtgcg gatcgcttcg acccagtcgt
     1861 gcagacgctc gatgctctgg gcctgcactt cgggcggccg ctggcggttg gcctgcaggc
     1921 gctgcaggtt gccgccggcg cagaagacct ggccttcgcc ggtcagcacc acgcagcgca
     1981 cctcggggtt cgactcggcg gcgctcagcg cctcgacgcc ggcggcgtac atctcgggcc
     2041 cgagcgcgtt gcgatgctcg gggttgctga tggtcagcac catggtgctg ccttcgaagg
     2101 tgctgcgcaa ttcggcggtc atgagggtgg tggagtgggc tgaacggatt caaacatccc
     2161 tacgcagagg gcgcgaaagt ttcgcagaga acgcaaagga gacaacttcc atttgagggc
     2221 cgtggccgac ttctgcgtcg ctcactgcgg attccacggc ctccaacatg cctggtgctg
     2281 tctgctgacc ctttgtcttc gcgcgcggcg cctcattgcc gtggcgccgc agtcctttac
     2341 ccgcccccgg tggatgctct tgcttgtgcg acttctgcgc agtcttggcg tcctctgcgt
     2401 acggatgttt ccagcattca ggcttcctcg tgcagcagcg acagcccgat ggcgccgcgg
     2461 cggcgcagcc aggggctggg gcggtagcgc gggtcgccgt agacggtctg caggttgaac
     2521 agcacttcca gcatgttggc cgggccccag cggtcgccca tggccagagg cccgagcggg
     2581 tagcccaggc ccagggtgac ggcggtttcc agatcctgcg ggctgcacac gcgctgctgg
     2641 cacatgtcgg cggcgatgtt gacgatgtgg gccaccaccc gctgggtgac gaagccgccc
     2701 gaatcgtgga tcacgctcac cggcttgccg tcgcgcgcga acagggcgtg ggcggcgtcg
     2761 cgcatgtcgc tgcgcgtggc agggttggtg gccagcacgc ggcggcgggt ggcggcgtct
     2821 tcgatcagca ggtcgatgcc gacggtgcgc gccggatcca gccgctcgac cacggccacc
     2881 gtggtgacgt cgaaacccag tggcgccacc agcgtcagcg cctgcggcga gggcgactgg
     2941 ccggtttcga tgcgggcgcc caggtccttc agcagctggt acagctccgc acggcgtgcg
     3001 gcgcgggtcg atacccacac cggcggcagc tccgccacct gcggcgcggc gggctccggc
     3061 gccacctgcg cgctgccgcc caggtagcgg taaaaacctt cgccactctt gcgacccagc
     3121 acgccgccgg ccagccgctg cgcggtgatg acgctgggac ggtagcgcgg ttcgtcgaaa
     3181 tactggcggt agatcgactc catcaccggg tgcgaaacgt ccagcgcggt caggtcgaac
     3241 agctcaaacg ggcccagacg aaagcccacc tggtcgcgca ggatgcgatc gacggtgacg
     3301 aaatcggtca cgccttcgcc cacgatgcgc agcgcctcgg tgccgtagcc gcgcccggcg
     3361 tggttgacga tgaaacccgg cgtgtcggcc gcctgcaccg gcgtgtggcc catctgacgc
     3421 gcgtagtcgg tcaggcgctg gccggtggcg gcgtcggtct tgaggccggc gatgacctcg
     3481 accaccttca tcagcggcac cgggttgaag aagtggtagc cggccagtcg gcccgggtgc
     3541 ttgagccccg cggcaatcgc cgtcaccgac agcgaggagg tgttggtggc cagtatggcg
     3601 tcgggccgca cgatgccttc cagctcggca aacagcgtct gcttgacgtc caggcgctcg
     3661 acgatggcct cgatcaccag gtcgcagtcg gccagatcgg ccagcgtggc ggccggcggc
     3721 acgcgggaga catggctgtc ggcctgctca cgcgtcagac ggcctttgtc gaccagcttg
     3781 tcccactgcg ccatcagtgc ttctcgggcc ttggcaacgg cgtcgggctg ggtgtcgtac
     3841 aggcgcacgg tgctgccggc ctgggcggcg atctgggcaa tgccgcgccc catggcgccg
     3901 gtgccgacga tgccgacgcg ggaaaagacg ggtgtgttca tggcgacaca ttatggcggc
     3961 agcgcgcgcc gcgtcgcagc ccacagaaac tatcaaaaca atagctgctg gcgctggcca
     4021 ggtgggcgcc ggaggccgat ttgttgagta cctcctgcgg tggctggcga ggcctcagcg
     4081 ccgcatctgc agggcgcccg ggttgacgat ctgcgtgggt gtgccgcgca ggtagttgac
     4141 cacgttgtcg aaggcggcgc cgaagtacag ctcgtagctg tcctgctcca cgtagccgat
     4201 gtgcggggtg cagatgcagt tctccagccg cagcagggcg tggccctgca ggatgggctc
     4261 gctctcgaac acgtccaccg ccgccatgcc ggggcggccg cggttgagcg cgctgaccag
     4321 cgcgtcgtgc tcgatcagct cggcgcgcga ggtgttgacc agcagggcgg tgggcttcat
     4381 gcggctgagg tcttccagcc gcacgatggc gtgcgtggcc tcgaccagcc gcaggtgcag
     4441 cgacagcacg tccgacaggg cgaagaactc ggcccgcgaa ggcgccacct ggtggccgtc
     4501 caggcgcgcg cgcgtcatcg aatccgacga accccacacc agcacctgca tgccgaaggc
     4561 gcgcccgtag ccggccacga tctggccgat gcgcccgtag ccccagatgc ccagggtttt
     4621 gccgcgcagc acctgcccga ggccgaagtt gggcggcatg gacgccgact tcagccccga
     4681 ctgctgccag gcgccgtgct tgaggttgcc gatgtactgc ggcagccgcc gcatggcggc
     4741 catgatcagc gcccaggtca gctcggccgg cgccaccggc gagcccacgc cttcggccac
     4801 ggcgatgccg cgctcggtgc aggcggccac gtcgacgtgg ctgcccacgc ggccggtctg
     4861 cgaaatcatc ttcagcctgg ggagcttttc gatcagctgg cgggtgatct gcgtgcgctc
     4921 gcggatcagc accagcacct cggcatcgcg cagacgcacc gacagctggc ccaggccctt
     4981 gacggtgttg gtgtacacct tggccgggta gggctccagc cgggacgcgc aggccagctt
     5041 gcgcacggcg tcctggtagt cgtcgaggat cacgatgttc atgcgcgcat tgtgcctctg
     5101 gccagcggcc agctgcgcac gcgcatgggg gccgaccgct tctcgggcgg cgtccggacg
     5161 gggcgaattc gaggcccgcg tcaggcgctg aatacgaagc gcgtgccggt ggaggagcgc
     5221 acgaagcgat cgcgcatttc cgactgcgcc atcgagatga aggtttcgcc ggagcagtgc
     5281 atggggatca ggaagtcggg gttgatggcc ttcaattcgg ccagggtttg ccgctgatac
     5341 tccggcgtgt gcggggcaag gtgaaaaccg cccagaatcg cgtggacctt gtcggtgccg
     5401 gccacctgca tggcgcggcg caccgaattg acgatgccgc gatggccgca ggaggtcatg
     5461 acgaccaggc cgcgcccttt cagctggtag acaatggcct gctcatgctc gaagtcgtcc
     5521 ggcaccgctg cggtcaggct ctgcttggcc ggcgacaggc cttccggcgc acaacccagc
     5581 ccagcttgga tgccaggcgt catgcgtgtg ggtgccagca cacgttcgaa cgaactgctg
     5641 gggatgaccc cggtggtgaa cccgtgcgag gccaggatcg aggggcgctg ggcgaacgtc
     5701 atggtcatgc ccgactgacg aatggcttcg cggtccagcg cgccaaaact ccctgcgtca
     5761 cccggcacgt tcagctcgcg cgaacagaag cactcttcac ttccgagata gaagggaagc
     5821 ccaggcttga gtttgctctt gtgagcgtcg agaaagccca ccatgccacc gaaatgatcg
     5881 tagtgaccgt gactcagggc gatggcgtcg agtttggaca catcgatgcc gcacagcagc
     5941 aggttgttga gcaaggcttc gggggtgtag gcaaagtcga gcagtacctg acgtacttcg
     6001 tcgccccgcc ctgattcgat gtgcagcgac aggccccact cgttctgcag cgtacgacgg
     6061 ggcggccgac caggtttgac atcgaagccc agtcgctgca cctgcaggtt gcccagcgtg
     6121 cgcggtggct cgaaggcatg gtggtacgag tcggtcacca ctcggatcgc cacgcgatcg
     6181 accacgggca cgtccgcagg cttgtcgatg gttgcagcca ccgccacacg gccaccgaac
     6241 aaaccgcctg cggccagcaa ggacagcgag cctgcgcctg ccctcagcag ttgccgccgt
     6301 ccggcgctgg cgaggtagct gtcgtcggtg gtggtttcaa tggtcatggc tggcttcctc
     6361 cgggttgatt gcggcgattc atgctatcgg cttcagggtg cgttgtcacg gattgccgca
     6421 aagagggcgc cgcagaaccc gccgccaggc ccatttgtcg acacgacacc acgcgccccg
     6481 gtggggcacg attcatgcgt cttggttcat gcgcagcgcg gttgtggcgg gctgttcgcc
     6541 agttcggccg cggccagccg ctccagctcg gcgcagacgt cgatcaggcg gcccgaaatg
     6601 cgcaggcgcg ccggcgcgtc cggggcctgg ggatcgcgca ggcggagggt gcgtacgggt
     6661 ggcgcgctgc agggggcggc ggtcggggcg ggatccgggt ggagggggag accgccggcg
     6721 ccagtttgca gcgcgcgctg ccgggccagg cctgaccagg cgttgtcggg ggcgggcctg
     6781 atcagcgagc tgccggcgct gcccgggcgg cgcgggcgcc ggtagctggg cacccggtag
     6841 tcgtccaggc acaggacggt ggccgcggtg ggctggtggg tggtgaatcg ggccatgtga
     6901 aacctttcag agtgaatcag gttggacaca ggcgagattg cggtgaagag gcgccgcccc
     6961 agggtgtgga tggcccggcc atctgcacgc ccgccacctg tggggcgccg tgactgccgc
     7021 gctacatcag catgctgttg cggatcaggc cgacggccag gccctcgatt tcgaacggct
     7081 cgcccggctg caccacgatg gtggggtagt cggggttttc ggcatgcagc tcgatctggt
     7141 gctggtggcg atgaaagcgc ttgaccgtga cgtcgtcacc caggcgcgcc accacgatct
     7201 ggccgttgcg tgcttcgcgt gtggcgtgca ctgccagcag gtcgccgtcg aggatgccgg
     7261 cgccctgcat cgacatgccg cgcacccgca acaggtagtc ggggcgctgg gtgaacaggg
     7321 tgggttcgag gtggtaggtt tgctccacgt gctcctgcgc caggatgggc gagccggcgg
     7381 ccacgcgacc gaccagcggc aacgccagtt gcgccatgcc gggcagcgtc aggctgaagg
     7441 cgtcgcccgc caggcggctg cgggccgcgc ctttcaggcg gatgccgcgc gaagtgccgc
     7501 tgacgagttc gatcacccct ttgcgtgcca gagcctgcag gtgttcttca gccgcgttgg
     7561 cggacttgaa gcccagctcg gccgcgattt ctgcgcgcgt gggcggcgcc ccggtgcgcg
     7621 cgatggcgtg ttgcaccagt tgcaggatct gctgctggcg ggcggtgagc ttgggcatgt
     7681 cggacatggt ggctgacctc aggtgtctgg aatctggatc gatccacagt gactgtgatt
     7741 ttacacagtt actgcgcgat gacaagcggt ggttgatcgc cccacggcca gccggatatg
     7801 ttacatatgt gtatttaagt attgcttatg attgaattcg aatggccatc gagcccgggc
     7861 cacaacgttg caatcgagga gacacatcgt catgcaagcc acccccatct ccccgttcgg
     7921 tcgcactgcg cgtgtggccg tgttgagcgc actgtgcgcc gctgtggccg catgtggcgg
     7981 cggaggtgac gcggaccccg cacccgcacc cgcacccgca cccgcacccg cacccgcgcc
     8041 tgcgcctgcg cctgcgcctg cgcctgcgcc tgcgcccgca cccgcacccg cacccgcacc
     8101 cgcacccgca cccgcacccg cacccgcac
//
LOCUS       DAOOOG010000119         8092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_10437_length_8092_cov_23.546846, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000119 DAOOOG010000000
VERSION     DAOOOG010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10437_length_8092_cov_23.546846"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..926
                     /locus_tag="PK242_09335"
     CDS             3..926
                     /locus_tag="PK242_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ94262.1"
                     /translation="MAYACLGLSMALVGSYVALSKPLAAVFPVLLLAWLRFGIGGVAM
                     LHWLRKPADEPPLGGRMRGLLFLESFLGNFLFTLCMISGVKLTTAVSAGVIMAAIPAA
                     VALLSWLFLRERVAPRVWAGVVCAVLGIALVSLSKQELTPHAASALDPSDSGDLRWLG
                     NLLLVGAVLCEASYAVIGKALTGQLSPRRITALINLWGFALSTPFGLWLAWRFDFGSV
                     APGMWALLVFYALAACMWTVWLWMTGLKSVPAAQAGVFTVLLPVSAAAVGVLALGERL
                     SLLQLLAYGVALAGVLLATLPSRPAWRVGRR"
     gene            complement(1301..1801)
                     /gene="rraA"
                     /locus_tag="PK242_09340"
     CDS             complement(1301..1801)
                     /gene="rraA"
                     /locus_tag="PK242_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HOZ94263.1"
                     /translation="MTSVPFSTCDLCDAHEQDSSGAFRVLPPVFHDFGAVAPFCGPVH
                     TLRAWEDNSRVREAVNAPGAGRVLVIDGGGSLRRALVGGNLAVAAASNGWAGIVVDGC
                     VRDVAELRAAGIPIRALALMPLRTEKRGEGQAGEPVQVQGVWVRPGDWLYADADGMVL
                     ADRALH"
     gene            2078..2467
                     /locus_tag="PK242_09345"
     CDS             2078..2467
                     /locus_tag="PK242_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HOZ94264.1"
                     /translation="MATAKKAPAKKAAPAKKAAPAKKAAPAKKAAPAKKAAPAKKAAP
                     AKKRTPSAAFMKAMTPSAALAAVIGSKALPRTEVTKKVWEYIKANKLQDSVNKRNINA
                     DAKLKPIFGKAQVTMFEMTKLISNHLS"
     gene            complement(2591..4279)
                     /locus_tag="PK242_09350"
     CDS             complement(2591..4279)
                     /locus_tag="PK242_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOZ94265.1"
                     /translation="MTSRIAGHLLVDCLLAQGVSHVFGVPGESYLAVLDGFYAQRERI
                     RFVICRQEGGAAFMAEAHGKLTGRPGVCFVTRGPGATNASIGVHTAAQDSTPMVLFVG
                     DVASDQRDREAFQEVDYRAFFGAFGMAKRVERVDSPERLPEYVARAFATALNGRPGPV
                     VLVLPEDMLTQPVPDALQPQPRWQVPAWLPDAGGLARLQALLQAAQRPIVIAGGSSWT
                     PEGARELQAFAEAWQLPVATALRFQDSFDNHHRLYAGDVGIAINPRLAERIRQADLVL
                     ALGPRLGEMTTSGYTLLQVPRPQQRLIHVHPGAEELGRVYQPELALCASLPAMAAALA
                     ALPAPASVPWRDWTEAAHADYLANLQAQALPGPLDMAAVIGTLQRLLPPDAALCNGAG
                     NFASWAHRFFRYHGLTKGVKTQLAPTNGAMGYGVPAAIACAITSGRVVVALTGDGDFM
                     MTGQELATARQYGARLIVVLVNNGVYGTIRMHQARAYPQRQAGSMLTNPDFVRLAESH
                     GLAGVRVERTEQFEPALRAALARSDGTLIEIMLSEQVITTRSTLEAIETQAAGR"
     gene            complement(4299..5531)
                     /locus_tag="PK242_09355"
     CDS             complement(4299..5531)
                     /locus_tag="PK242_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ94266.1"
                     /translation="MSTSAVSAAADARVTVPLREDARVIGLVGLAHASSHFAHLLLPP
                     LFPVFATEFGLSFSQLGLLMTVFFVISGVGQAMSGFLVDRVGARPVLFGSLLCFIVAS
                     LSGYFAQGWGGLLLVAVFAGLGNAPFHPVDFSILNHRVSGPRLGYAFSAHGLSGNLGW
                     ALTPVFLLFFTELSSWRTGYLAAALLYAGVLAVLVLQREHLHTEAVVHHAHGDAQGGG
                     AGFLRLPVVWWCFAFFLLSTLTLAVVQNFASPIMKAMHGVSFEAATTMITAYMLCGAL
                     GMLVGGFVAARMPAASDHVVAWAMGVGALLIVAAASGWLGGFGSMAVLAGTGFAVGVG
                     GPSRDLMIKKATPKGATGRIYGTVYSGLDIGFAIGPLIYGVFMDRGMYATTLVLAALF
                     LALSVAAALGVGRRTRLA"
     gene            complement(5719..6522)
                     /locus_tag="PK242_09360"
     CDS             complement(5719..6522)
                     /locus_tag="PK242_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOZ94267.1"
                     /translation="MTAANPSTPNLASLADELERTLKLRTPPFGMKLLASAESLKAIP
                     KLRRPQAVHTMDQIVAQAARLGWTLGVTAGDLVGDQCRAVVGLGGQDAAWYAGQPMAG
                     VWFATVEDSARHQAAMEVVPVGRHEALVVAPLAKWGGFGAGQDEPDIALFYATPGAMI
                     YFINGLQWSGYQRFDWSVVGESSCADSWGRALKTRQPSLSIPCFAERRYGGVLDDEML
                     MALPVADIARALAGMRALAKNGLRYPFAQYGIQQDVRAGMGVSYPKGSA"
     gene            complement(6615..>8092)
                     /locus_tag="PK242_09365"
     CDS             complement(6615..>8092)
                     /locus_tag="PK242_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ94268.1"
                     /translation="RYITDRFLPDKAIDLIDEAAAKIKIEIDSKPEVMDKLDRRMIQL
                     KIEREAVKKEKDEASQKHLAQIEEDLARLQKEYADLEEIWKAEKASAQGSEQVRKEIE
                     QIKFQIQEATRKGDFNHVAELQYGKLPALEKQLKEAQASEQGAEAQAKAHKLLRTQVG
                     AEEIAEVVSRATGIPVSKMMQGERDKLLKMEDALHQRVVGQDEAISAVANAIRRSRSG
                     LSDPNRPLGSFLFLGPTGVGKTELTKALAGFLFDSDQHMVRIDMSEFMEKHSVARLIG
                     APPGYVGYDEGGYLTEAVRRKPYSVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRT
                     VDFKNTVLIMTSNIGSPMIQAMAGQPYEDVKDAVFGELKNHFRPEFLNRIDEIVVFHG
                     LDAAQIGAIAKIQLKVLAERLARMELTLNVSDQALAELAKVGFDPVFGARPLKRAIQQ
                     RIENPLAKLVLEGKFPPKTEIEVSVDPIKDPGVFQFSSHTG"
BASE COUNT         1287 a         2832 c         2719 g         1254 t
ORIGIN      
        1 ccctggccta tgcctgcctg ggcctgtcga tggccctggt gggcagttac gtggcactgt
       61 ccaaaccgct ggcggcggtg tttccagtgc tgctgctggc ctggctgcgc tttggcatcg
      121 gcggcgtcgc catgctgcac tggttgcgca agccagccga tgagccgccg ctgggcgggc
      181 gcatgcgcgg tctgctgttt ctcgaatcct tcctcggcaa cttcctgttc acgctgtgca
      241 tgatcagcgg cgtgaagctg accacggcgg tgtcggccgg cgtcatcatg gcggccatac
      301 cggcggcggt ggcgctgctt tcctggctgt tcctgcgcga gcgcgtggcc ccgcgcgtgt
      361 gggccggtgt ggtgtgcgcg gtgctcggca tcgccctcgt gtcgctatcg aaacaggagc
      421 tgacgccgca cgctgcatct gccctggacc catccgacag tggtgacctg cgctggctgg
      481 gcaacctgct gctggtgggc gcggtgctgt gtgaggccag ctacgcggtg atcggcaagg
      541 cgctgaccgg ccagctgtcg ccgcgccgca tcacggccct gatcaatctg tggggctttg
      601 ccctgagcac cccattcggc ctgtggctgg cctggcgctt cgacttcggc agcgtggccc
      661 cgggcatgtg ggcgctgctg gtgttttatg cgctggccgc ctgcatgtgg accgtgtggc
      721 tgtggatgac cggtctgaag agcgtgccgg ccgcgcaggc cggcgtgttc acggtgctgc
      781 tgccagtcag tgcggcggcg gtcggcgtgc tggcgctggg cgagcgtctg agcctgctgc
      841 agctgctggc ctacggggtg gcgctggccg gcgtgctgct ggccaccctg ccctcacgcc
      901 ccgcctggcg cgtgggtcgg cgctgacgcg cgccgcgccc tgggcttcgg aatcttgcag
      961 tctgaaccgt tcaccctgag cctgtcgaag cgcttcgaca aattcagccc gaacggtatt
     1021 caagacatcc aacctgggtt caacctagaa acggcagttg accgcttgtc aacttcgggc
     1081 agaccgcatc gatgcgggat ctcgtggctt cgatggcttt cacctcttgg gtgagtacgc
     1141 tgcgcacccg cccgcaccgc gctcggcgcg ccatgcggcg ttgctcctgc ttgcgggcac
     1201 cagcccgcgg cggcgtcgcg tcttgcctgg cacgccgatc acggcacggg cgggcgcgtc
     1261 caactgccgt ttccaggttc aaaggccctg atccatggcc tcagtgcagg gcgcggtcgg
     1321 ccagcaccat gccgtcggcg tcggcgtaca gccagtcgcc aggccgcacc cacacaccct
     1381 gcacctgcac cggctctccg gcctgaccct cgccgcgttt ttcggtgcgc agcggcatca
     1441 gcgccagcgc gcggatgggg atgccggctg cgcgcagctc ggccacgtcg cgcacgcagc
     1501 catccaccac gatgccggcc cagccgttgc tggcggcggc caccgccagg ttgcctccca
     1561 ccagtgcgcg gcgcagcgag ccgccgccgt cgatcaccag cacgcggccg gcgccagggg
     1621 cattgacggc ttcgcgcacg cgcgagttgt cttcccacgc ccgcagcgtg tgcaccggcc
     1681 cgcagaacgg ggccacggcg ccgaagtcgt gaaacaccgg gggcagcacg cgaaaagcgc
     1741 cgctgctgtc ctgctcgtgc gcatcgcaca ggtcgcaggt ggaaaacggg acagaagtca
     1801 tggtctggat cggggtcaac ggtcaaaaaa tcgccggcgg cggcccgccg tggcgccgcc
     1861 cgaggataac ggagcgccac gcgctcgcgc accccaccgc tcaccagtgc acgcgaggcg
     1921 accgccgaca ctgaaaaaaa gcaacatctc cgagggaaaa accgcttgct ccccttggag
     1981 acgtgctact tatccagtag catgcgcatc agccagtggt gcaagcaggc aagctggcag
     2041 aagcaaggcg agttttcaac accaaaggat cgcaaccatg gcaactgcaa agaaagcgcc
     2101 ggccaagaag gccgcacccg ccaagaaggc ggcccccgcg aagaaggcgg cacccgccaa
     2161 gaaggccgct ccggccaaga aggcggcacc tgccaagaag gccgccccgg ccaagaagcg
     2221 cacccccagc gccgccttca tgaaggccat gacgcccagc gccgcgctgg ccgccgtgat
     2281 cggcagcaag gcgctgccgc gcaccgaggt caccaagaag gtgtgggagt acatcaaggc
     2341 caacaagctg caggacagcg tcaacaagcg caacatcaac gccgacgcca agctgaagcc
     2401 gatcttcggc aaggcccagg tcaccatgtt cgagatgacc aagctgatca gcaatcacct
     2461 atcgtgatcg acgtctggcc cttggggcca gaacggcgaa aaccggcccc caggggccgg
     2521 tttttttatg ccttataggc ctgcagcagc ctgctgacaa gcgccggcag ctatcaacaa
     2581 cggagcatcg tcaacgcccg gcggcctgcg tttcaatcgc ctccagcgtg ctgcgggtgg
     2641 tgatgacctg ctcgctcagc atgatctcga tcagggtacc gtcgctgcgg gcgagcgccg
     2701 cgcgcagggc gggttcgaac tgctccgtgc gctccacccg cacgccggcc agtccgtggc
     2761 tttcagccag ccgcacgaag tcggggttgg tcagcatcga gccggcctgg cgctgcgggt
     2821 aggcgcgcgc ctggtgcatg cggatggtgc cgtagacgcc gttgttgacc agcaccacga
     2881 tcagccgggc gccgtactgg cgcgcggtgg ccagctcctg cccggtcatc atgaagtcgc
     2941 cgtcgccggt cagcgccacc accacccgcc cgctggtgat ggcgcaggcg atggccgccg
     3001 gcacgccata gcccatggcg ccgttggtgg gcgccagctg ggtcttcacg cccttggtca
     3061 ggccgtggta gcggaagaac cggtgcgccc agctggcaaa gttgccggcg ccgttgcaca
     3121 gggcagcgtc cggcggcagc aggcgctgca gggtgccgat cacggccgcc atgtccagcg
     3181 gcccgggcag cgcctgggcc tgcaggttgg ccaggtagtc ggcgtgcgcc gcctcggtcc
     3241 agtcgcgcca cggcacgctg gcgggcgccg gcagcgccgc cagcgcggcg gccatggccg
     3301 gcaggctggc gcacagggcc agttcgggct ggtacacacg gcccagctcc tcggcgccgg
     3361 ggtgcacatg gatcaggcgc tgctgcggcc gcggcacctg cagcagggtg taaccgctgg
     3421 tggtcatctc gcccaggcgc ggccccagcg ccagcaccag atcggcctgg cggatgcgct
     3481 cggccagccg cgggttgatg gcgatgccga cgtcgcccgc gtacagccgg tggtggttgt
     3541 cgaagctgtc ctgaaagcgc agcgccgtcg ccaccggcag ctgccaggcc tcggcaaagg
     3601 cctgcagctc gcgcgcgccc tcgggcgtcc agctgctgcc cccggcgatg acgatcggac
     3661 gctgcgccgc ctgcagcagc gcctgcaggc gcgccagccc gccggcgtcg ggcagccagg
     3721 ccgggacctg ccagcgtggc tgcggctgca gcgcgtcggg caccggctgc gtcagcatgt
     3781 cttcgggcag caccagcacc accgggccgg gacggccgtt gagggcggtg gcaaaggctc
     3841 gcgccacgta ttcgggcagg cgctcggggc tgtcgacgcg ctcgacccgc ttggccatgc
     3901 cgaaggcgcc gaagaaggcg cggtagtcga cctcctgaaa ggcttcgcga tcgcgctggt
     3961 cgctggccac gtcgccgacg aacagcacca tcggcgtcga gtcctgcgcc gccgtgtgca
     4021 caccgatgct ggcgttggtg gcgcccggcc cgcgggtgac gaagcacacg cccggccgcc
     4081 cggtgagctt gccgtgcgct tcggccatga aggcggcgcc gccttcctgg cggcagatca
     4141 cgaaccggat gcgctcgcgc tgggcgtaaa agccgtccag caccgccagg tagctctccc
     4201 ccggcacgcc aaagacgtgg ctgacgccct gggccagcag gcaatcgaca aggaggtggc
     4261 cggcgatgcg agaggtcatc gcggcattgt gcttgaactc aagccagacg ggtgcggcga
     4321 cccacaccca gcgccgccgc caccgacagc gccaggaaca gcgccgccag caccagcgtg
     4381 gtggcgtaca tgccgcggtc catgaagacg ccgtagatca gcggtccgat ggcaaagccg
     4441 atgtccagcc ccgaatacac cgtgccgtag atgcgcccgg tggcaccctt gggggtggct
     4501 ttcttgatca tcaggtcgcg gctggggccg cccacgccga ccgcgaaacc ggtgcccgcc
     4561 agcaccgcca tggagccgaa gccacccagc cagccgctgg ccgccgccac gatcagcagc
     4621 gcgcccacgc ccatggccca ggccaccacg tggtcggacg ccgccggcat gcgcgcggcc
     4681 acgaagccgc ccaccagcat gcccagggcg ccgcacagca tgtaggcggt gatcatggtg
     4741 gtggccgcct cgaagctgac gccgtgcatg gccttcatga tgggcgaggc gaagttctgc
     4801 accaccgcca gcgtcagcgt ggacagcaga aagaaggcga agcaccacca gaccaccggc
     4861 aggcgcagaa agccggcgcc gccaccctgt gcgtcgccgt gcgcgtggtg caccaccgcc
     4921 tcggtgtgca ggtgttcgcg ctgcagcacc agcaccgcca gcacgccggc gtacagcagc
     4981 gccgccgcca ggtagccggt gcgccagctc gacagctcgg tgaagaacag caggaacacc
     5041 ggcgtcagcg cccagcccag gttgcccgac aggccgtggg cgctgaaggc gtatcccagt
     5101 cgcggccccg acacacggtg gttgaggatg ctgaaatcca ccgggtgaaa cggcgcgttg
     5161 cccaggccgg caaacaccgc caccagcagc agcccgcccc agccctgggc gaagtagccc
     5221 gacaggctgg ccacaatgaa gcacagcagc gagccaaaca gcaccggccg cgcgcccacc
     5281 cggtcgacca gaaagcccga catggcctgc ccgacgccgg agatgacgaa aaacaccgtc
     5341 atcaacaggc ccagttgcga aaacgacagc ccgaactcgg tggcgaacac cggaaacagc
     5401 ggcggcagca gcaggtgcgc aaagtgcgag ctggcgtggg ccagaccgac cagcccgatc
     5461 acccgcgcgt cttcgcgcag gggcacggtg acccgcgcat cggcggccgc ggagacggca
     5521 gaagtggaca tggacgcgaa tggtacgcgt ttgtgacacg ccccgccgcg cgccgcgcaa
     5581 gcaccggggc agcgcctggg gacatgcaat aaaacaagct gccgcgccca cctatcaagc
     5641 gccagcagct atcactatca tagcaattca gacgctgacc gggctgcggc gcggccgccc
     5701 cacccgccct ttgcaagctc aggcgctgcc cttggggtag ctcacaccca tgccggcgcg
     5761 cacgtcctgc tggatgccgt attgcgcaaa cggatagcgc aggccgttct tggccagcgc
     5821 ccgcatgccg gccagcgccc tggcgatgtc ggcgacaggc agcgccatca gcatctcgtc
     5881 atccagcacg ccgccgtagc ggcgctcggc aaagcagggg atcgacaggc tgggctggcg
     5941 cgtcttgagc gcccggcccc acgaatcggc acaggacgac tcgcccacca cgctccagtc
     6001 gaagcgctga tagcccgacc actgcaggcc gttgatgaag tagatcatgg ccccaggcgt
     6061 ggcgtagaac agcgcgatgt cgggctcgtc ctgcccggcg ccaaagccgc cccacttggc
     6121 cagcggcgcc accaccagcg cctcgtggcg gcccaccggc accacctcca tcgccgcctg
     6181 gtggcgggcc gagtcctcca ccgtggcgaa ccacacgccc gccatcggct ggccggcata
     6241 ccacgcggcg tcctgcccgc ccaggccgac cacggcgcgg cattgatcgc ccaccaggtc
     6301 gccggcggtg acgcccagcg tccagcccag gcgcgccgcc tgggcgacga tctggtccat
     6361 ggtgtggacg gcctgcgggc ggcgcagctt gggaatggcc ttcagcgatt cggccgacgc
     6421 cagcagcttc atgccgaacg gcggcgtgcg cagcttgagc gtgcgctcca attcgtcggc
     6481 caggctggcc agattgggcg tggacggatt ggcagcggtc atggcggtac tctggcacaa
     6541 aaaaaaggga cgtggccatt gtggcgcacg tccccgctgg tgtgtgtcgc gatcgtcgcg
     6601 cggtggctga ttcttcagcc ggtgtggctg ctgaactgaa acacccccgg atccttgatc
     6661 ggatcgaccg acacctcgat ctcggtcttg ggcggaaact tgccctccag caccagtttg
     6721 gccagcggat tctcgatgcg ctgctggatg gcgcgcttga gcggccgggc gccgaacacc
     6781 gggtcgaacc cgaccttggc cagctcggcc agcgcctggt cggacacgtt cagcgtcagc
     6841 tccatgcgcg ccagccgctc ggccagcacc ttgagctgga tcttggcgat cgccccgatc
     6901 tgcgccgcgt ccaggccgtg gaagacgacg atctcgtcga tgcggttgag gaattcgggg
     6961 cggaagtggt tcttcagctc accgaacacc gcgtccttca cgtcttcgta gggctggccg
     7021 gccatggcct ggatcatcgg gctgccgatg ttgctggtca tgatcagcac ggtgttcttg
     7081 aagtccaccg tgcggccctg gccgtcggtc aggcgcccgt cgtcgagcac ctgcagcagc
     7141 acgttgaaga cgtcggggtg ggccttttcg atctcgtcga acagcaccac gctgtagggc
     7201 ttgcgccgca ccgcttcggt caggtagccg ccctcgtcat agcccacgta gcccggcggc
     7261 gcgccgatca ggcgcgcgac gctgtgcttt tccatgaatt cgctcatgtc gatgcgcacc
     7321 atgtgctggt cgctgtcgaa caggaaaccc gccagcgcct tggtcagttc ggtcttgccg
     7381 acacccgtgg ggccgaggaa caggaaggag ccgagcgggc ggttcgggtc cgacaggccc
     7441 gagcgcgagc ggcggatggc gttggccacg gccgagatgg cttcgtcctg acccaccacg
     7501 cgctggtgca gcgcgtcttc catcttcagc agcttgtcgc gctcgccctg catcatcttg
     7561 ctgaccggga tgccggtggc gcggctgacc acttcggcga tctcttcggc gccgacctgg
     7621 gtgcgcagca gcttgtgcgc cttggcctgg gcctcggcgc cctgctcgct ggcctgcgct
     7681 tccttgagct gcttttccag cgccggcaac ttgccgtact gcagctcggc gacgtggttg
     7741 aagtcgccct tgcgcgtggc ttcctggatc tgaaacttga tctgctcgat ctccttgcgc
     7801 acctgctcgc tgccctgggc gctggctttt tcggccttcc agatttcttc caggtcggcg
     7861 tattccttct gcagccgcgc caggtcttcc tcgatctgcg ccaggtgctt ctgcgaagcc
     7921 tcgtcctttt ccttcttgac ggcctcgcgc tcgatcttga gctggatcat gcggcgatcg
     7981 agcttgtcca tcacctcggg cttggaatcg atctcgatct tgatcttggc cgccgcctcg
     8041 tcgatcaggt cgatcgcctt gtcgggcagg aagcggtcgg tgatgtagcg gt
//
LOCUS       DAOOOG010000120         8045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_10513_length_8045_cov_19.867584, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000120 DAOOOG010000000
VERSION     DAOOOG010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10513_length_8045_cov_19.867584"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /gene="urtB"
                     /locus_tag="PK242_09370"
     CDS             complement(<1..1262)
                     /gene="urtB"
                     /locus_tag="PK242_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HOZ94269.1"
                     /translation="MKILRTAWTSALIGALGLLLALPGHALTAEQAFAMAAAEDTEAR
                     VAAINAATLEPDAGGRLAAYLQALADDAVRTSGGKAYVVQGDAASDPVTGAVATLPAD
                     AEEVINNNYLRGVIEAAQAALALTGGDAQARRDAARKFAEEPDAARLSLIEKVLARET
                     DAGVKALLARARAASLLGSEDAAQRLQAVQQLAQLKTPETQLLLNQRLRDEADPAVQK
                     AIRAALSAVAESLAWGERLGAVFSGLSLGSVLLLAALGLAITYGLMGVINMAHGELIM
                     IGAYATYLVQALFRRWLPESLFDAYLVVAVPAAFLAAALVGAVLERGVIRFLYGRPLE
                     TLLATFGISLVLQQTVRSLFGAQNVGVENPAWLSGGVALLPNLTLPYNRIAIIAFALA
                     VLGAVAFLIARTRLGLFVRGVTQNRAMAS"
     gene            complement(1361..2641)
                     /gene="urtA"
                     /locus_tag="PK242_09375"
     CDS             complement(1361..2641)
                     /gene="urtA"
                     /locus_tag="PK242_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HOZ94270.1"
                     /translation="MFKRRSAMKTLAAAAAVASLSLTGLSAQAADTIKVGVLHSLSGT
                     MAISETVLKDTVLMAIDDINAKGGVLGKKLEAVVVDPASNWPLFAEKAKQLLTQDKVA
                     VVFGCWTSVSRKSVLPVFEQNNGLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVEYL
                     MSKEGGAAKRFVLLGTDYVYPRTTNKILRAFLKSKGVKDADIMETYTPFGHSDYQTIV
                     ADIKKFSTGGKTAVVSTINGDSNVPFYKELGNAGLKAKDVPVVAFSVGEEELRGVDTK
                     PLVGHLAAWNYFMSVKNPTNSAFIKQWSDYAKAKGIAGHKDKPLTNDPMEATWVGIHM
                     WKQAVEKAKTTDVDKVIAAMAGQTFTAPDGFTVKMDEKNHHLHKPVMVGEIKADGQFG
                     VVWKTKGPIKAQPWSPFIPGNDKKLDEPDGKSSS"
     gene            2946..3248
                     /locus_tag="PK242_09380"
     CDS             2946..3248
                     /locus_tag="PK242_09380"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HOZ94271.1"
                     /translation="MELSPREKDKLLIFTAALLAERRKARGLKLNYPEAVALISAFVM
                     EGARDGRTVAELMSAGRTVLGRADVMEGVPEMIPDIQIEATFPDGTKLVTVHQPIV"
     gene            3283..3882
                     /locus_tag="PK242_09385"
     CDS             3283..3882
                     /locus_tag="PK242_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HOZ94272.1"
                     /translation="MKKIIPLALTSLPVAAFAHMGEQAHTHGLANSFLQGFAHPFTGA
                     DHLAAMLAVGAWSALTFPRLGSAWRVPTAFVALLVAGAVAGLAGLWMPGVEPMIAASV
                     LVLGLLVAVQKQMPWVLAAALAGVFAFFHGAAHGQELSGNAGLAALGALAGMALGSAL
                     LHVAGMAAGHGIMQRRQWLARLAGAATALLGAALLVRLV"
     gene            3893..4198
                     /locus_tag="PK242_09390"
     CDS             3893..4198
                     /locus_tag="PK242_09390"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HOZ94273.1"
                     /translation="MIPGELLTDDGAHALNPGRRTRTLVVHNASDRPIQVGSHYHFAE
                     TNAGLTFDRAAAHGMRLNIASGMAVRFEPGQQRTVELVDIAGARRVFGFRGLVQGDL"
     gene            4198..5919
                     /gene="ureC"
                     /locus_tag="PK242_09395"
     CDS             4198..5919
                     /gene="ureC"
                     /locus_tag="PK242_09395"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HOZ94274.1"
                     /translation="MASIDRRAYAETYGPTEGDRVRLADTDLIVEVEQDLTLAAAGGY
                     GEEVKFGGGKTIRDGMAQSQRTRAEGAVDTVLTNALIIDHWGIVKADIGLKDGRIAAI
                     GKAGNPDTQPGVQIVIGPGTEIISCEGLIVTAGGIDTHIHFICPQQIEEALASGITTM
                     IGGGTGPATGTFATTCTPGPWHIERMLQAADAFAMNLGFLGKGNTSLPGALHEQVAAG
                     AIGLKLHEDWGSTPAAIDNCLTVAEETDTQVAIHTDTLNESGFVEDTVAAFKGRTIHT
                     FHTEGAGGGHAPDILKVVGEPHVLPSSTNPTRPYTINTLDEHVDMLMVCHHLDAGIAE
                     DLAFAESRIRKETIAAEDILHDIGAISMFSSDSQAMGRVGEVILRTWQTAHKMKLQRG
                     ALAGDGARNDNFRIQRYLAKYTINPAIAHGISHEVGSLEVGKWADIVLWKPAFFGVKP
                     ACILKGGSIAMAAMGDPNASIPTPQPVHYRPMFGAFAGSKARSSLTFVSQAGLAAGIG
                     ERFGLHKPLAAVKGIRGVQKRHMIHNDLAPHMEVDAQTYAVRANGELLVCEPATSLPM
                     AQRYFLF"
     gene            complement(5904..6425)
                     /locus_tag="PK242_09400"
     CDS             complement(5904..6425)
                     /locus_tag="PK242_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOZ94275.1"
                     /translation="MSISRDFSSTRTPAPQWQPCGAAHVAVYGTLRAGGINDIARLGS
                     GVVCVGTTRLSGRLHDLGWYPGLRLGGAQQVLAEVYAINPAIEQAMDRIEGLWPEDLG
                     EYAKRLLTLPVALAGGGTQTLTVLVYEALPATVAQAPVIEADDWLAWATQQGKAVRHT
                     DEAFALNAAQNRK"
     gene            6515..7033
                     /gene="ureE"
                     /locus_tag="PK242_09405"
                     /pseudo
     CDS             6515..7033
                     /gene="ureE"
                     /locus_tag="PK242_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
     gene            7188..7847
                     /locus_tag="PK242_09410"
     CDS             7188..7847
                     /locus_tag="PK242_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HOZ94276.1"
                     /translation="MWLASPALPIGGFSYSEGLEAAVQAAHVTTEDEAARWLVQQLHL
                     TQARGDMAAVAQALAAWRHGDLARVQQLNDWVLATRETSELRLQTEQMGRSLTEWLKN
                     RHTGHAAAMPQVQWLAARNPSYPMAFALAAHLTAAQTRDALLAYAFGWAENMVQAAIK
                     AVPLGQNAGQRILARLTGEIPAAVAHAQALPDAARQAFSPMLAILSARHEHQYSRLFR
                     S"
     gene            7844..>8045
                     /locus_tag="PK242_09415"
     CDS             7844..>8045
                     /locus_tag="PK242_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOZ94277.1"
                     /translation="MTSSALHHIPHRTRKLPPLRVGIGGPVGSGKTTLLEMLCKAMRD
                     KWDLVAITNDIYTKEDQRLLTIS"
BASE COUNT         1340 a         2862 c         2610 g         1233 t
ORIGIN      
        1 gaggccatgg cgcggttctg cgtgacgccg cgcacgaaca ggcccagccg cgtgcgggcg
       61 atcaggaagg ccacggcgcc cagcaccgcc agcgcgaacg cgatgatggc gatgcggttg
      121 tagggcagcg tgagattggg cagcagcgcc acgccgccgc tcagccaggc ggggttctcc
      181 acgcccacgt tctgcgcgcc gaacaggctg cgcaccgtct gctgcagcac caggctgatg
      241 ccgaaggtgg ccagcagcgt ctccagcggc cggccgtaga gaaagcggat cacgccgcgt
      301 tcgagcaccg cgcccaccag cgcagccgcc agaaaggccg ccggcacggc caccaccaga
      361 taggcgtcga acaaagactc gggcagccag cggcggaaca gcgcctgtac caggtaggtc
      421 gcatacgcgc cgatcatgat cagctcgcca tgcgccatgt tgatcacgcc catcagcccg
      481 taggtgatgg ccaggcccag ggcagccagc agcagcaccg agcccaggct caggccgctg
      541 aagacggccc ccagccgctc gccccaggcc agggattcgg ccaccgccga cagcgccgcc
      601 cggatggcct tctgcacggc cgggtcggcc tcgtcacgca ggcgctggtt cagcagcagc
      661 tgggtttcgg gcgtcttgag ctgggccagt tgctgcaccg cctgcaggcg ctgcgcggcg
      721 tcttcgctgc ccagcaggct ggccgcgcgg gcgcgcgcca gcagggcctt gaccccggcg
      781 tccgtctcac gggccagcac cttctcgatc agggacaggc gcgcggcatc gggctcttcg
      841 gcgaacttgc gcgccgcgtc gcggcgggcc tgcgcatcgc caccggtcag cgccagcgcg
      901 gcctgggccg cctcgatcac gccgcgcagg tagttgttgt tgatgacttc ctcggcatcg
      961 gcaggcagcg tggcgacggc gcccgtgacc gggtcgctgg ccgcgtcgcc ctgcaccaca
     1021 taggccttgc cccctgaggt gcgcacggcg tcgtcggcca gggcctgcag gtaggccgcg
     1081 agccggccgc ccgcgtcggg ctccagcgtg gcggcgttga tggctgccac gcgcgcctcc
     1141 gtgtcttcgg ccgccgccat ggcaaacgcc tgctcggccg tcagcgcatg gcccggaagg
     1201 gccagcagca ggccgagtgc gccgatcagg gcactggtcc atgcggtgcg cagaattttc
     1261 atagtcaaaa ccagccccag cgcttgtcca acaagcgcga acagctacaa aagaaatagt
     1321 aaacacctgg ccgacagagc gccggccagg cgccctgcga tcaggagctg ctcttgccgt
     1381 ccggctcgtc cagcttcttg tcgttgccgg ggatgaacgg gctccagggc tgggccttga
     1441 tcgggccctt ggttttccag accacgccga actggccgtc ggccttgatc tcgccgacca
     1501 tcacgggctt gtgcagatgg tggttcttct cgtccatctt gacggtgaag ccgtcggggg
     1561 cggtgaaggt ctggccggcc atggcggcga tgaccttgtc cacatcggtg gtcttggcct
     1621 tctccaccgc ctgcttccac atgtggatgc cgacccaggt ggcctccatc gggtcgttgg
     1681 tcagcggctt gtccttgtgg cccgcgatgc ccttggcctt ggcgtagtcg ctccactgct
     1741 tgatgaaggc gctgttggtg gggttcttca cgctcatgaa gtagttccac gcggccaggt
     1801 gccccaccag gggcttggtg tccacgccgc gcagctcctc ctcgcccacg ctgaaggcca
     1861 ccacgggcac gtccttggcc ttgaggccgg cattgcccag ctccttgtag aagggcacgt
     1921 tggagtcgcc gttgatggtc gagaccacgg ccgtcttgcc gcccgtggag aacttcttga
     1981 tgtcggcgac gatggtctgg tagtcgctgt ggccaaacgg ggtgtaggtc tccatgatgt
     2041 ccgcgtcctt cacgcccttg gacttgagga aggcgcgcag gatcttgttg gtggtgcgcg
     2101 ggtacacata gtccgtgccc agcagcacga agcgcttggc cgcgccgcct tccttgctca
     2161 tcaggtattc gacggcggga atggcctgct ggttgggcgc ggcgcctgtg tagaacacgt
     2221 tcttggacaa ctcctcgccc tcgtactgca cggggtagaa cagcaggccg ttgttctgct
     2281 cgaacaccgg cagcaccgac ttgcgcgaga cgctggtcca gcaaccaaag accacggcca
     2341 ccttgtcctg cgtcagcagc tgcttggcct tctcggcgaa cagcggccag ttggaggccg
     2401 ggtccaccac cacggcttcg agcttcttgc cgagcacgcc gcccttggcg ttgatgtcgt
     2461 cgatggccat cagcaccgtg tccttgagca cggtctcgga gatcgccatg gtgcccgaga
     2521 ggctgtgcag cacgccgacc ttgatggtgt ctgccgcctg tgcgctcagg cccgtgagcg
     2581 acaggctggc gacagccgcc gcggcggcga gggttttcat ggcggaacga cgcttgaaca
     2641 tggcttaacc tttcttgaat gcggggttgt ccccaggatg cccaggaacc atttcccggc
     2701 ttcgaagtac ctccaagcaa gccgcatgcc atgcccgacc ccagtgccac acgctacagc
     2761 agcaccgaag cttggaccgt cagaccagca ccaaaccagt gcatgtacgc accacaagcg
     2821 ttcattcggc aagcacatgc ccgcaggtcg cacatcgcgt gagtacagaa ttgccatcac
     2881 gcacccgaca cctgctggca cggcccttgc atcgtgcaac cccatgaacc tgtgagcgag
     2941 acgacatgga actctccccc cgcgaaaaag acaagttgct gatcttcacc gccgccctgc
     3001 tggccgagcg ccgcaaggcg cgcggactca agctgaacta ccccgaggcg gtggcgctga
     3061 tcagcgcctt cgtgatggag ggcgcgcgcg acggccgcac cgtggccgaa ctgatgagcg
     3121 ccggccgcac ggtactgggg cgcgccgacg tgatggaggg cgtgcccgag atgattcccg
     3181 atatccagat cgaggccacc tttcccgacg gcaccaaact ggtcacggtg caccagccca
     3241 tcgtttgagc catcgccccc aaccctttca ttcactgcaa ccatgaaaaa aatcatcccc
     3301 cttgccctga ccagtctgcc cgtcgccgca ttcgcccaca tgggcgagca ggcgcacacg
     3361 catggcctgg ccaacagctt tctgcagggc ttcgcccacc ccttcaccgg cgccgaccac
     3421 ctggcggcca tgctggccgt gggcgcgtgg agcgcactga cctttccccg cctggggtcg
     3481 gcctggcgcg tgccgaccgc cttcgtggcg ctgctggtgg ccggcgcggt ggcgggcctg
     3541 gccggcctgt ggatgccggg ggtggagccc atgatcgcag cctcagtgct ggtgctgggg
     3601 ctgctggtgg cggtgcaaaa acagatgccc tgggtgctgg ccgcggcgct ggcgggcgtg
     3661 tttgccttct ttcatggcgc ggcgcatggc caggaactga gcggcaacgc cggcctggcg
     3721 gcgcttggcg cgctggcggg catggccctg ggctcggcgc tgctgcacgt ggccggcatg
     3781 gcggccggcc acggaatcat gcaacgccgc cagtggctgg cgcggctcgc cggtgcggcc
     3841 acggcgctgc tgggcgccgc cttgctggtc cgcctggtct gaggagcgtc ccatgattcc
     3901 cggagaactg ctgaccgacg acggcgcgca cgccctcaac ccgggccgcc gcacccgcac
     3961 gctggtggtg cacaacgcca gcgaccggcc catccaggtg ggctcgcact atcactttgc
     4021 cgaaaccaat gccgggctca ccttcgaccg cgccgccgcg cacggcatgc ggctgaacat
     4081 cgccagcggc atggcggtgc gcttcgagcc gggccagcag cgcacggtcg aactggtgga
     4141 catcgccggc gcgcgccgcg tgtttggttt tcgcggtctg gttcagggag atctgtgatg
     4201 gccagcatcg accggcgcgc ctacgccgaa acctatggcc ccaccgaggg cgaccgcgtg
     4261 cggctggccg acaccgacct gatcgtcgag gtggaacagg atctgaccct ggccgccgcc
     4321 ggcggctacg gtgaagaagt gaagttcggc ggcggcaaga ccatccgcga cggcatggcg
     4381 caaagccagc gcacccgcgc cgagggcgcg gtggacacgg tgctgaccaa tgcgctcatc
     4441 atcgaccact ggggcatcgt caaggccgac atcggcctga aggacgggcg catcgccgcc
     4501 atcggcaagg ccggcaaccc cgacacccag ccgggcgtgc agatcgtcat cggccccggc
     4561 accgagatca tcagctgcga ggggttgatc gtgacggccg gcggcatcga cacgcacatc
     4621 cacttcatct gcccgcagca gatcgaagaa gccctggcca gcggcatcac caccatgatc
     4681 ggcggcggca ccggcccggc caccggcacc tttgccacca cctgcacgcc agggccgtgg
     4741 cacatcgaac gcatgctgca ggccgccgac gcctttgcca tgaacctggg ttttctgggc
     4801 aagggcaaca ccagcctgcc gggcgcgctg cacgagcaag tggctgccgg cgccatcggc
     4861 ctgaagctgc acgaggactg gggcagcacg ccggcggcca tcgacaactg cctgacggtg
     4921 gccgaggaga ccgacaccca ggtcgccatc cacaccgaca cgctcaatga gagcggcttt
     4981 gtggaggaca cggtggccgc cttcaagggc cgcaccatcc acacctttca caccgaaggc
     5041 gcgggcggcg gccacgcacc cgacatcctg aaagtggtgg gcgagcccca tgtgctgccc
     5101 agctccacca accccacccg cccctacacc atcaacacgc tggacgagca tgtggacatg
     5161 ctcatggtct gccaccacct ggacgccggc attgccgaag acctggcctt tgccgaaagc
     5221 cgcatccgca aggagaccat cgcggccgag gacatcctgc atgacatcgg cgccatcagc
     5281 atgttcagct ccgacagcca ggccatgggc cgcgtgggcg aggtgatcct gcgcacctgg
     5341 cagaccgcac acaagatgaa gctgcagcgc ggtgcgctgg cgggcgacgg cgcacgcaac
     5401 gacaacttcc gcatccaacg ctatctcgcc aaatacacca tcaacccggc catcgcccac
     5461 ggcatcagcc acgaagtggg cagcctggag gtgggcaagt gggccgacat cgtgctctgg
     5521 aagccggcct tcttcggcgt caaaccggcc tgcatcctca agggcggcag catcgccatg
     5581 gcggccatgg gcgaccccaa tgccagcatc cccacgcccc agccggtgca ctaccggccg
     5641 atgttcggcg cgtttgccgg cagcaaggcg cgcagctcgc tcacctttgt ctcgcaggcc
     5701 gggctggcgg cgggcatcgg cgagcgcttc ggcctgcaca agccgctggc ggcggtcaag
     5761 ggcatacgcg gcgtgcaaaa gcgccacatg atccacaacg acctggcgcc gcacatggaa
     5821 gtcgatgccc agacctacgc cgtgcgcgcc aatggcgagc tgctggtgtg cgaacccgcc
     5881 accagcctgc ccatggcgca gcgctacttc ctgttctgag ccgcgttcag cgcaaacgcc
     5941 tcgtccgtat gccgcaccgc cttgccctgc tgcgtggccc aggccagcca gtcatccgcc
     6001 tcgatcaccg gcgcctgcgc cacggtggcg ggcagggctt cgtagaccag caccgtcagg
     6061 gtctgcgtgc cgccgcccgc cagcgcgacg ggcagggtca gcaggcgctt ggcgtattcg
     6121 cccagatctt cgggccacag gccttcgatg cggtccatgg cctgctcgat cgcggggttg
     6181 atggcataga cctcggccag cacctgctgc gcaccgccca gcctcaggcc cggataccag
     6241 cccagatcat gcaggcggcc cgacagccgg gttgtgccca cgcagaccac gccgctgccc
     6301 agccgcgcaa tgtcattgat gccgcctgcg cgcagcgtgc cgtagaccgc cacgtgcgcg
     6361 gcgccgcagg gctgccactg gggcgcgggc gtccgtgtcg aagaaaaatc acgcgagatt
     6421 gacatgcctg tcatgctatc gggttgcaca ggccaagggt gatgcacaaa gcgcgccaac
     6481 cggccctggc agccttctcc atccaacgta caccatgctg actgtcaaca aacttcttcc
     6541 gcgcggccag ggcctcgccg gccccctgct ccagcgcgcc gccagcgtcg agctggactg
     6601 ggatgtgcgc cagaaatccc gctttgcagc caccgacagc cagggccgcg cgctggcggt
     6661 gtttctgccg cacggtcagg ccgtgcgcgg tggcgacgtg ctggtggccg aagacggctc
     6721 gctgatccgc gtgcaggcag caaagcagaa ggtgctgcac atcacggcct gcgccgagca
     6781 cggcagcacc ttcgacctga tgcgcgccgc ctaccacctg ggcaaccgcc atgtaccgat
     6841 cgagctgcag cccgaccacc tgaagatcga gcccgaccat gtgctggccg acatgctgcg
     6901 cgccatgcac atgaccgtgg tcgaggccga gttgccgttc gaacccgaag gcggtgccta
     6961 cggtgggcat gtgacccacg acgggcacag ccaccatcac cacggccatg ggcatcacca
     7021 tgagcacggc cactgaaagc gcggcgggcg cggcccgccg cgcggccgct ccgaaggcgt
     7081 ccccgccccc gacttgtccg gtgacccttc cccggactga agcagccgac ttggcgccgc
     7141 aggaaagcac agaggttccc gtagcccccg ccacgctgct gcagctgatg tggctggcgt
     7201 cgcccgcgct gcccatcggc gggttttcgt attctgaagg gctggaagcg gccgttcagg
     7261 ccgcgcatgt cacgaccgag gacgaagccg cgcgctggct ggtgcagcaa ctgcacctga
     7321 cccaggcccg cggagacatg gccgccgtcg cccaggccct ggccgcctgg cgccatggtg
     7381 acctggcccg cgtgcagcag ctcaacgact gggtgctggc cacgcgcgag accagcgagc
     7441 tgcgcctgca gaccgagcag atgggccgct cgctgaccga atggctgaaa aaccgccata
     7501 ccggccatgc tgccgcgatg ccccaggttc agtggctggc cgcccggaac cccagctacc
     7561 ccatggcgtt tgccctggcc gcgcacctga ccgcggccca gacccgcgac gccctgctgg
     7621 cctatgcctt cggctgggcc gaaaacatgg tgcaggcggc catcaaggcc gtgccgctgg
     7681 ggcagaacgc aggccagcgc atcctggccc ggctgaccgg tgaaattccc gccgccgtgg
     7741 cgcacgccca ggctctgccg gatgccgcgc gccaggcctt ctcgcccatg ctggccatcc
     7801 tctcggcccg gcacgagcac cagtactcac gccttttcag atcatgacct cgtctgcgtt
     7861 gcaccacatc ccccaccgca ccaggaagct gcccccgctg cgggtgggca tcggcgggcc
     7921 cgtcggctcg ggcaagacca ccctcctgga gatgctgtgc aaggccatgc gcgacaagtg
     7981 ggacctggtg gccatcacca acgacatcta caccaaggaa gaccagcgcc tgctgaccat
     8041 cagcg
//
LOCUS       DAOOOG010000121         8039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_10523_length_8039_cov_28.965306, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000121 DAOOOG010000000
VERSION     DAOOOG010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10523_length_8039_cov_28.965306"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PK242_09420"
     CDS             complement(<1..593)
                     /locus_tag="PK242_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOZ94278.1"
                     /translation="MRLFTSPRAPNPRRVLMFMHEKGIAGVEQVTVDLNAGEHQSAAY
                     RAKVPVARVPALELPDGRVLSETRAICSYLEGLHPEPNLMGEGFDERAFIEMHDRRVE
                     WYWLLPIANCVRHTHPGLAALEQPQFPDFGRSQGQKVRESARWLDEALARQPWVAGER
                     FTIADITAFCALEFARLMKFSAGAEGFAALQAWRDRVA"
     gene            complement(681..1169)
                     /locus_tag="PK242_09425"
     CDS             complement(681..1169)
                     /locus_tag="PK242_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOZ94279.1"
                     /translation="MTSRRWKPAVTVAAVIEQQGRYLLVEERTAAGLQLNTPAGHLEP
                     GESPQAGAVREALEETGRLFAPSALLGVYLAASTDAQGQPATWLRLAFCGTAGEPDPA
                     RRLDQGIVRTLWLTPAEIEASRSRHRSPLVWRCVQDHLRGQRFDLSAVVMDPSALGEP
                     PS"
     gene            1342..2502
                     /locus_tag="PK242_09430"
     CDS             1342..2502
                     /locus_tag="PK242_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOZ94280.1"
                     /translation="MLIGVPAETAAGETRVAVTPETAKKLVAQGHVVRVQAGAGLRAA
                     AIDEAYRTAGAEITDAAGALGAEMVLKVRSPAPAELAQIQPGSVLVGMLEPFNAEGLQ
                     RLAAASLTAFALEAAPRTTRAQSMDVLSSQANIAGYKAVIMAADRYQKFFPMLMTAAG
                     TVKAARVVILGVGVAGLQAIATAKRLGAVIEASDVRPSVKEQVESLGAKFIDVPYETA
                     EEKEAAEGVGGYARPMPQSWLERQKVEVAKRVAQADVVISTALIPGRAAPVLITEEMV
                     QSMKPGSVIIDMAAGKGAPNADGTVGGNCPLTEADRTVVKHGVTIVGETNLAAQVAAD
                     ASALYARNVLDFLKLVLPPAAKGEPPATFRLDLEDDIVAACLMAHQGAVTRK"
     gene            2499..3320
                     /locus_tag="PK242_09435"
     CDS             2499..3320
                     /locus_tag="PK242_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94281.1"
                     /translation="MSAARLTRSRGMLALAASALLAACAAPGGETSATGVAAPTASVA
                     APHIQPPPTSVGTWVELGHVQAPWLAGDAPVPVSGPVAPTRVAGLRRDDGRWLAIVMV
                     QRATGTAVPCPRPTGVSVATEGDGCLRLRRDADFDRWLERQHGVLHQWVESRGWAARP
                     RAWVAYRLPHTGSQALEAHVLLDPALIEPTTRGSADFLAAGQPGLDWARALAAATRTA
                     DGSGVLALPALPFAPPVTPVAPPVATPAVVTAPPAARTPPPPPRPAATAPRRDRG"
     gene            3373..3690
                     /locus_tag="PK242_09440"
     CDS             3373..3690
                     /locus_tag="PK242_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOZ94282.1"
                     /translation="MDAVSPTLINLIIFVLAIYVGYHVVWTVTPALHTPLMAVTNAIS
                     AIVIVGAMLAAALTEGSFAKLLGVAAVALAAVNIFGGFLVTRRMLEMFKKKDRPAKAE
                     AAK"
     gene            3703..5139
                     /locus_tag="PK242_09445"
     CDS             3703..5139
                     /locus_tag="PK242_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOZ94283.1"
                     /translation="MSMNLVTLLYLIASVCFIQSLKGLSHPTTSIRGNLFGMVGMTIA
                     VLTTAALIMRLAGSALGLGWVLLGLVLGGGYGAWRAKTVEMTKMPELVAFFHSMIGLA
                     AVFIAGATVIEPWAFGLAKSVAQGGDGQIPGGNRIELALGAFIGAVTFSGSVIAWGKL
                     SGKSKFRLFQGAPVQFAGQHWVNLGLGLLTAFFIFGFWHSQSHLDFALVCLLGFIIGV
                     TLIIPIGGADMPVVVSMLNSYSGWAAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCK
                     AMNRSFFNVILGGFGGDAAAAAGGAQEQRPVKTGSADDAAFMLSNADSVIIVPGYGLA
                     VARAQHAVKELVDKLTDKGIDVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDVN
                     GEFGQADVAIILGANDVVNPAALQKGSPIYGMPILEAYKAKTVIVNKRSMAAGYAGLD
                     NELFYMDKTMMVFGDAKKVVEDIGKAIE"
     gene            5297..7003
                     /locus_tag="PK242_09450"
     CDS             5297..7003
                     /locus_tag="PK242_09450"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HOZ94284.1"
                     /translation="MTERSWLKHYPAGVPADIDTTQYSSLVALIEESFSKYADRTAYS
                     FMGKDVSYADTDADSRALAAYLQSLGLQRGDRVALMMPNVPQYPASVAAVLRAGFVVV
                     NVNPLYTARELEHQLKDAGAKAIIILENFAATLQQCIARTQVKHVVLASMGDRLGGLK
                     GMIVNLVVRKVKKMVPPFSLPGAVRFNDALARGRSAPFAAPPIGPDDLAVLQYTGGTT
                     GVAKGAMLLHRNLIANVLQSEAWNDPVMKSIPAGEQPTSVCALPLYHIFAFTVNMMLS
                     MRTGGKTILIPNPRDLPAVLKELSKHTFHSFPAVNTLFNGLANHPDFNTVNWKNLRVS
                     VGGGMAVQSAVAKLWLEKTGCPIVEGYGLSETSPSASCNPVTSKEFSGTIGLPLPSTY
                     LKILDDSGAEVPLGQPGEIAIKGPQVMAGYWQRPDETAKVMTPDGYFKSGDIGIMDER
                     GYTRIVDRKKDMILVSGFNVYPNEIEDVVAAIPGVLEVGAVGVPDEKMGEAIKLVIVK
                     KDPGLTEEAVRAYCKQNLTGYKQPRVIEFRAELPKTPVGKVLRRELRDPAPAKPAAAS
                     VD"
     gene            7028..7816
                     /locus_tag="PK242_09455"
     CDS             7028..7816
                     /locus_tag="PK242_09455"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOZ94285.1"
                     /translation="MALPPGASDGGRPRRTGVVAAMHPELAAVLERLPDEQRQRVAGR
                     DFWVGHWHAHEVVAVLSGVGKVAAATTATLLIERFGVDALLFTGTAGGLGDGVRVGDL
                     VLAGEFLQHDMDASPLFPRFEVPLYGRARFATDAALSARLGAAARQVLARASDTLGAH
                     AVAEFALHAPAVHRGLVVSGDRFVATSAESAALRQALPEALAVEMEGAAVAQVCHDYG
                     LPFAALRTVSDRADDSAHVDFMRFIDQVASRYSAAVLEAFFAEL"
     gene            complement(7882..>8039)
                     /locus_tag="PK242_09460"
     CDS             complement(7882..>8039)
                     /locus_tag="PK242_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOZ94286.1"
                     /translation="LPPTLVASIYGMNFKAMPELDWQLGYPYAIALMIASALVPMWYF
                     RRRGWLK"
BASE COUNT         1277 a         2876 c         2743 g         1143 t
ORIGIN      
        1 gccacccggt cgcgccaggc ctgcagggcg gcaaagcctt cggcgccggc gctgaacttc
       61 atcagcctgg cgaattcgag ggcgcagaag gccgtgatgt cggcgatggt gaaacgctcg
      121 ccggccaccc agggctggcg cgccagcgcc tcgtccagcc agcgggcact ctcacgcact
      181 ttctgaccct gcgaccggcc gaagtcgggg aactgcggct gctccagcgc cgccaggccg
      241 gggtgggtgt ggcgcacgca gttggcgatc ggcagcagcc agtaccactc cacccgccgg
      301 tcgtgcattt cgatgaaggc gcgctcgtcg aagccctcgc ccatcaggtt cggctcgggg
      361 tgcaaacctt ccagatagct gcagatggcg cgcgtttcgc tcagcacgcg accatcgggc
      421 agctccagcg ccggcacgcg cgccaccggc accttggcgc ggtaggccgc ggactggtgt
      481 tcgcccgcgt tcaggtcgac cgtcacctgt tccacgccgg cgatgccctt ttcgtgcatg
      541 aacatcagca cgcggcgcgg gttgggggcg cggggggaag tgaacagtcg catggtttgc
      601 tcctataaat atagctgctt gcgcttacca ggcaagcgcc agagcccaaa aatcattgtc
      661 aaccacgggc ggcggcacct tcaggacggc ggctcgccaa gcgccgacgg gtccatcacc
      721 accgccgaca gatcgaagcg ctggccgcgc aggtggtcct gcacgcagcg ccacaccagc
      781 gggctgcggt gccgtgaccg gctggcttcg atttcggccg gggtcaacca cagggtgcgc
      841 acgatgccct ggtccagccg gcgcgccggg tcgggctcgc ccgccgtgcc acagaaggcc
      901 aggcgcagcc aggtggcggg ctggccctgg gcgtcggtcg aggccgccag gtacacgccc
      961 agcagggcgc tgggcgcgaa caggcggccg gtttcctcca gcgcctcgcg cacggccccg
     1021 gcctggggcg actcgcccgg ctccaggtgg ccggccgggg tgttcagttg cagaccggcg
     1081 gcggtgcgct cttccaccag caggtagcgg ccctgctgct cgatcacggc agccacggtg
     1141 accgcgggtt tccatcggcg tgacgtcatg ccagccatta tcggcagcgc cgcgccgcgg
     1201 tcgccgtcgc ggcaggcggc gcaccaaaat gacgcacaat cgccgccaag agcgcgcagc
     1261 cgcgcactcg atcaccgaca aacccgccca gccggcccgc gcccgagcgg accgatggct
     1321 ggctcacgag aggagtgttt catgctgatt ggcgtacccg ccgagacggc ggccggcgaa
     1381 acccgggtgg ccgtgacccc ggaaacggcc aagaagctgg tggcccaggg ccatgtcgtg
     1441 cgtgtgcaag caggcgctgg cctgcgcgcg gccgccatcg acgaggccta ccgcaccgcc
     1501 ggcgccgaaa tcaccgatgc cgccggcgcc ctgggcgccg agatggtgct gaaggtgcgc
     1561 agccccgcgc ccgcagagct ggcgcagatc caacccggca gcgtgctggt gggcatgctg
     1621 gagccgttca atgccgaagg cctgcagcgc ctggcggccg ccagcctgac cgcttttgcg
     1681 ctggaagcgg cgccgcgcac cacgcgcgca cagagcatgg acgtgctgtc cagccaggcc
     1741 aacatcgccg gctacaaggc cgtcatcatg gcggccgacc gctaccagaa gttcttcccc
     1801 atgctgatga ccgccgccgg caccgtcaag gccgctcgtg tggtcatcct gggcgtgggc
     1861 gtggccggcc tgcaggcgat tgccaccgcc aagcgcctgg gcgcggtgat cgaagccagc
     1921 gacgtgcgcc ccagcgtgaa ggaacaggtc gagtcgctgg gcgccaagtt catcgacgtg
     1981 ccctatgaaa ccgccgagga aaaggaagcc gccgagggcg tgggcggcta cgcccggccg
     2041 atgccgcaga gctggctgga gcgtcagaag gtcgaagtgg ccaagcgcgt ggcccaggcc
     2101 gacgtggtga tctccaccgc cctgatcccc ggccgcgccg cgccggtgct gatcaccgaa
     2161 gagatggtgc agagcatgaa accgggctct gtcatcatcg acatggcggc cggcaagggc
     2221 gcacccaacg ccgacggcac ggtgggcggc aactgcccgc tgaccgaggc cgaccgcacc
     2281 gtggtcaagc acggcgtcac catcgtcggc gaaaccaacc tcgccgccca ggtggcggcc
     2341 gacgcgtcgg cgctgtacgc gcgcaacgtg ctggacttcc tcaaactggt gctgccgccg
     2401 gccgccaagg gcgagccgcc ggccaccttc aggctggacc tggaagacga catcgtcgcc
     2461 gcctgcctga tggcgcacca gggcgcggtg acccgcaaat gagcgccgca cgcctgacgc
     2521 gcagccgggg catgctggcg ctcgccgcca gcgccctgct ggccgcctgc gcggcgcccg
     2581 ggggcgagac ctccgccacc ggcgtggccg caccgacggc cagcgtggcg gcgccgcaca
     2641 tccaaccgcc gcccaccagc gtcggcacct gggtcgagct cggccacgtg caggccccct
     2701 ggctggccgg tgacgccccg gtgcccgtca gcggcccggt ggcgccgacc cgcgttgccg
     2761 gcctgcgccg ggacgatggt cgctggctgg ccatcgtgat ggtgcagcgc gccaccggca
     2821 ccgccgtgcc ctgcccgcgc cccaccgggg tgtcggtcgc caccgagggc gacggctgcc
     2881 tgcgcctgcg gcgcgacgcc gacttcgacc gctggctgga acgccagcat ggcgtgctgc
     2941 accagtgggt cgaaagccgc ggctgggcgg cacgcccgcg cgcctgggtc gcctaccggc
     3001 tgccgcacac cggcagccag gcgctggaag cccatgtgct gctggacccg gccctgatcg
     3061 aaccgaccac ccgcggcagt gccgactttc tggccgccgg ccagccgggc ctggactggg
     3121 cgcgcgcact ggccgccgcc acccggaccg ccgacggcag cggcgtgctg gcgctgccgg
     3181 ccctgccctt cgcgccgccg gtcacgcccg tcgcaccgcc ggtcgccacc ccggccgtcg
     3241 tgaccgcccc gccggcggca cgcaccccgc caccaccgcc gcgcccggcg gccaccgcgc
     3301 cgcgccgcga tcgcggctga gtcggccacc gcaccgacac gcacccgata ccaccatccc
     3361 gaggagccct gcatggacgc cgtatccccc accctcatca acctgatcat cttcgtgctg
     3421 gccatctacg tgggctacca cgtggtgtgg accgtgacac cggcgctgca cacgccgctg
     3481 atggcggtca ccaacgccat ttcggccatc gtcatcgtcg gcgccatgct ggcggcggcg
     3541 ctgaccgagg gcagctttgc caagctgctg ggcgtggccg cggtggcgct ggccgcggtg
     3601 aacatcttcg gcggctttct ggtgacgcgc cggatgctgg aaatgttcaa gaagaaagac
     3661 cgtcccgcca aggcggaagc ggccaagtaa ggagcgaccg acatgagcat gaacctcgtc
     3721 acgctgctgt acctgatcgc cagcgtctgc ttcatccagt ccctcaaggg gctgagccac
     3781 cccaccacct cgatccgggg caacctgttc ggcatggtcg gcatgaccat cgccgtgctg
     3841 accacggcgg cgctgatcat gcggctggcc ggctcggcgc tgggcctggg ctgggtgctg
     3901 ctgggcctgg tgctgggcgg cggctatggc gcctggcgcg ccaagacggt cgagatgacc
     3961 aagatgcccg agctggtggc gtttttccac agcatgatcg gcctggcggc ggtgttcatc
     4021 gccggcgcca cggtgatcga gccctgggcc ttcggcctgg ccaagagcgt cgcccagggc
     4081 ggcgacggcc agatcccggg cggcaaccgc atcgagctgg cgctgggcgc cttcatcggc
     4141 gcggtcacct tcagcggctc ggtgatcgcc tggggcaagc tgtcgggcaa gagcaagttc
     4201 cgcctgttcc agggcgcgcc ggtgcagttt gccggccagc actgggtcaa cctggggctg
     4261 ggcctgctga ccgcgttctt catcttcggc ttctggcatt cgcaaagcca cctggacttc
     4321 gccctggtgt gcctgctggg cttcatcatc ggcgtgacgc tgatcatccc gatcggcggc
     4381 gccgacatgc cggtggtggt gtcgatgctg aacagctact ccggctgggc ggcggcgggc
     4441 atcggctttt cgctcaacaa cagcatgctg atcattgccg gctcgctggt gggcagctcg
     4501 ggcgcgatcc tgagctacat catgtgcaag gcgatgaacc gctcgttctt caacgtcatc
     4561 ctgggcggct tcggcggcga tgcggcggcc gctgcaggcg gcgcgcagga gcagcgcccg
     4621 gtcaagaccg gcagcgccga cgatgcggcc ttcatgctga gcaacgccga cagcgtgatc
     4681 atcgtgcccg gctacggcct ggcggtggcg cgcgcgcagc acgccgtcaa ggaactggtc
     4741 gacaagctca ccgacaaggg catcgacgtc aagtacgcca tccacccggt ggccggccgc
     4801 atgccgggcc acatgaacgt gctgctggcc gaggccgagg tgccctacga ccaggtgttc
     4861 gagatggaag acgtcaacgg cgagttcggc caggccgacg tggccatcat cctgggcgcc
     4921 aacgacgtgg tcaacccggc cgcgctgcaa aagggcagcc cgatctacgg catgccgatc
     4981 ctggaggcct acaaggccaa gaccgtgatc gtcaacaagc gctccatggc cgccggctac
     5041 gccggcctgg acaacgagct gttctacatg gacaagacca tgatggtgtt cggcgacgcc
     5101 aagaaggtgg tcgaagacat cggcaaggcc atcgagtaag tccacgccgg ccgtcgctgc
     5161 ggcgccacga ccgggaaaac gccggtttgc ccctgcccgc gcgcgttgca caatgtgaca
     5221 ggtggccgcg cagcgcgccg ccttcccatt gcctgaccgt gccgggcacg gtccgacctc
     5281 aaggagtacc ccatccatga ccgagcgcag ctggctcaag cactaccccg ccggcgttcc
     5341 cgccgacatc gacaccaccc agtacagctc cctggtggcg ctgatcgagg agagcttctc
     5401 gaagtacgcc gatcgcaccg cctacagctt catgggcaag gacgtcagct acgccgacac
     5461 cgatgccgac agccgcgctc tggccgccta cctgcagagc ctgggcctgc agcgcggcga
     5521 ccgcgtggcc ctgatgatgc ccaacgtgcc gcagtacccg gccagcgtgg cggcagtgct
     5581 gcgcgccggc ttcgtggtgg tcaacgtcaa tccgctgtac accgcgcgcg aactcgagca
     5641 ccagctcaag gacgccggcg ccaaggccat catcatcctg gagaacttcg ccgccacgct
     5701 gcagcagtgc atcgccagga cccaggtcaa gcacgtggtg ctggccagca tgggtgaccg
     5761 gctgggcggc ctcaagggca tgatcgtcaa cctggtggtg cgcaaggtca agaaaatggt
     5821 gccgccgttc agcctgccgg gcgcggtgcg cttcaacgat gcgctggcgc gcgggcgcag
     5881 cgcgcccttc gcggcgcccc cgatcggccc cgacgatctg gcggtgctgc agtacacggg
     5941 cggcaccacc ggcgttgcca agggcgccat gctgctgcac cgcaacctga tcgccaacgt
     6001 gctgcaatcc gaggcctgga acgacccggt gatgaagagc atcccggccg gcgagcagcc
     6061 cacctcggtg tgcgcgctgc cgctctacca catcttcgcc ttcacggtga acatgatgct
     6121 gtccatgcgc accggcggca agaccatcct gatccccaac ccgcgcgacc tgccggcggt
     6181 gctcaaggag ctgtccaagc acaccttcca cagctttccg gcggtcaaca cgctgttcaa
     6241 cggcctggcc aatcacccgg acttcaacac cgtcaactgg aagaacctgc gcgtctcggt
     6301 gggcggcggc atggcggtac agagcgcggt ggccaagctg tggctcgaaa aaaccggctg
     6361 cccgatcgtc gaaggctacg gcctgtccga aaccagcccc tcggccagct gcaacccggt
     6421 cacgtcgaag gagttctccg gcaccatcgg cctgccgctg cccagcacct acctcaagat
     6481 cctggatgac agcggggccg aggtaccgct cggtcagccg ggcgaaatcg ccatcaaggg
     6541 cccgcaggtg atggccggct actggcagcg ccccgacgag accgccaagg tcatgacgcc
     6601 cgacggctac ttcaagagcg gcgacatcgg catcatggac gagcgcggct acacccgcat
     6661 cgtcgaccgc aagaaggaca tgatcctggt cagcggcttc aacgtctacc ccaacgagat
     6721 cgaggacgtg gtggccgcca ttccaggcgt gctggaggtc ggcgcggtcg gcgtgcccga
     6781 cgaaaagatg ggcgaagcca tcaagctggt gatcgtcaag aaggaccccg gactgaccga
     6841 ggaagcggtg cgcgcctatt gcaagcagaa cctcaccggc tacaagcagc cgcgcgtgat
     6901 cgagttccgc gccgagctgc ccaagacccc cgtcggcaag gtgctgcggc gcgagctgcg
     6961 cgacccggcg cccgccaagc cggccgccgc ttcggtggat tgagcgccac cgacgccccg
     7021 cgcacgcatg gcgctgcccc ctggcgcgag tgacggcggc cggccgcgcc gcaccggcgt
     7081 ggtggcggcc atgcatcccg aactggcggc cgtgctcgag cgcctgccgg acgagcagcg
     7141 ccagcgtgtg gccgggcgcg acttctgggt cggtcactgg cacgcccacg aggtggtggc
     7201 ggtgctgtcc ggggtgggca aggtggcggc ggccaccacc gccacgctgc tgatcgaacg
     7261 cttcggcgtc gacgccctgc tgttcaccgg caccgccggt gggctgggcg acggcgttcg
     7321 ggtcggcgat ctggtgctgg ccggcgagtt cctgcagcac gacatggacg cctcgccgct
     7381 gtttccgcgc ttcgaggtgc cgctgtacgg ccgcgcgcgc tttgccaccg acgctgcgct
     7441 gtcggcgcgc ctgggcgccg ccgcgcgcca ggtgctggcc cgcgccagcg acacgctggg
     7501 cgcccatgcc gtggccgaat tcgccctgca cgcgccggcc gtgcaccgcg gcctggtcgt
     7561 cagcggcgac cgcttcgtcg ccaccagcgc cgaaagcgcg gcgctgcgcc aggcgctgcc
     7621 cgaggcgctg gcggtggaaa tggagggcgc cgccgtggcc caggtgtgtc acgactacgg
     7681 gctgcccttc gccgccctgc gcacggtgag cgaccgcgcc gacgacagcg cccacgtcga
     7741 tttcatgcgc ttcatcgacc aggtggccag ccgctacagt gccgcggtgc tggaagcgtt
     7801 ttttgcagaa ctatgatttc gatagctgct ggcgcttgac ccgagcgcgc cagaggccga
     7861 tttgaccttc aaaccgagga atcacttcag ccagccacgg cggcggaaat accacatcgg
     7921 caccagggcg ctggcgatca tcagcgcgat ggcataggga tagccgagct gccagtccag
     7981 ctccggcatg gccttgaagt tcatgccgta gatgctggcc accagggtgg gcggcagca
//
LOCUS       DAOOOG010000122         8026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_10548_length_8026_cov_16.882073, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000122 DAOOOG010000000
VERSION     DAOOOG010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10548_length_8026_cov_16.882073"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1137)
                     /locus_tag="PK242_09465"
     CDS             complement(127..1137)
                     /locus_tag="PK242_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOZ94287.1"
                     /translation="MDSFFLMGARPLVEGSPSMKLHELLDWGAIAAQLKGLYQREISG
                     AGGPEPYSPLGMFKLMLLGQWHGLSDAQLEQALRVRLDFMVFTGFEPSAGELPDASTI
                     CRFRNRLVKAELDQKLLTLINSQLEQRGLKVQGARGAIIDATIIPSAARPRQHVEGEG
                     EQARIVDSADADARWVKKGKQAFFGYRGHTAVDSEDGYVEHVQVHPANEAEINKLPEI
                     VEALSPGVEAVLADKGYASKANRQWLQERGMGDLIQHKGSAGKPVHPLLKAFNQKIGG
                     IRFKVEQAFGTMKRRFHLARARYFGTAKVQAQMCWAALGMNLLKAHRKLKRMELAGAG
                     AP"
     gene            1525..4896
                     /locus_tag="PK242_09470"
     CDS             1525..4896
                     /locus_tag="PK242_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HOZ94288.1"
                     /translation="MLANHVVTPDPVPAGGIATITMTVQNNGTGAASNVVLTDTIPAG
                     STFVDMSASDGGTCTASAPYHCDWASIPFPGSRTVTLRVRLPSAQVWTNAATVTSSTA
                     DPNQGNNSLSRTITVQQAADLAVTATSSAGGTIAAGTPYTYTLNVANNGPDPLPAGQS
                     PTVTFNVPSGSSITSRPGGTGWDCQPASGYPLTDEPGPPVGAQITCTRSDGLANGSSF
                     PPITVNAVGNVTGAVDATFDVSSNYLDGNTTNNTATVSQPLSAGTDMSIRKTVALAAG
                     TSGTQATFTLRARQEGGAAPTGVVVTDTLPAGMTYVSHNAPAPWNCTWAAPLLTCTYP
                     GTYTGGAFTDLPDIQLVADVTATGTIPNTGHVTATPPDPNGTNNSSTVEVTNTADLRI
                     TKSASLSPVVTGQPYNWNIVVANYGPMPVLAGQYITVTETIPAGMTINSASGTGWACY
                     SDAGYATAAVYPVAGGATLYCRQLRTSNLGFNATNGTATNTLTLAAVNTVAGSLNNSA
                     CLGLSGNGPNEAGGAPDFQRNCHGVGTNGTLPENSADLEIVKTVVGPNPVVVGQPLTY
                     RLVARNLSTSVNATQVHVYDTVNNLRNIAGFPGLVSVTTTQGTCAPATPASVTSASID
                     CNLGTLNAGASATIDITIRPNNTTATDLTRGNTATVNSLDVGDPNRANNTSTVSSVVQ
                     PRVDMTVSKSVNPTPVRVGQPMTYTVTANNAGPSEATNVRITDVMPPNTAFISVGTPS
                     NGGTCGTVPAADSTGGTLECTWASVPAGGNRTVTFTVRPLEAALGGGTTGTINNTVTV
                     ATDSTETNLDNNSASIPAEVIASLVDILVQKTDSVDPVVLGATTRYTITIRNAGPSYG
                     TNLVMTDTFPNAGNTARFSYQGGLRATAAGTAVTPVCTEPAIGAISGTLTCTFPTIAV
                     GAANEVVLQYDMRAESIVTAGDYSGTQGNHVAVAVAENETEMGNNQVDEHTTTRREAV
                     PTDLGLTKAVDKATMAAGEQVVYTLTVRNNGPQESRGAQVIDTLPAGMSFVASPDGCV
                     NSAGTVTCAVGTLPVGETRVFTFTAQLADPYNGPAALVNNARLDAPGDIDLSNNEDEV
                     TTRVPPPSTPIPTLSQWGLILLSLLMAGFALRRRV"
     gene            5067..5291
                     /locus_tag="PK242_09475"
                     /pseudo
     CDS             5067..5291
                     /locus_tag="PK242_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(5418..6218)
                     /locus_tag="PK242_09480"
     CDS             complement(5418..6218)
                     /locus_tag="PK242_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOZ94289.1"
                     /translation="MQVAAWPSALGHRLIVWLRWLCVSWLFLAGSAFSQGLTDPAVQR
                     GVAWLQGQIQASGQLASEAGSPALPMQVRSEAAITLRRLAQTAPPALYTAIEGATPDT
                     TEYLARKAIGKQLAAVSDATHLDALIKLQNADGGFGAAAGLASNAQDTAWALRALAAN
                     QASSAAAVKALGWLLSAQQADGQWKLVPDGDAVVTTALAVQALTLYRQQPAVQAALGK
                     ARTWLTGQRTAQQTWGDDLRTAHALLGMNLLKAHRKLKRMELAGAGAP"
     gene            complement(7028..7945)
                     /locus_tag="PK242_09485"
     CDS             complement(7028..7945)
                     /locus_tag="PK242_09485"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HOZ94290.1"
                     /translation="MSKEIGEASSEDADQGQGEKARGLGRGLMPIHHPNRDFFLCDLF
                     DYALKDDGVSMEAPIFTLATKPDTKIWRWQSRDGNRSVEVTPSVKGRATQFDKDLLIY
                     VVSQMTDALNRGRPDANNRTVRFRVYDYLVSTNKLTGGKEYQRLEDALERLRGTSITT
                     NIRTGGQRVKEGFGIVDSWTIIEKSPDDSQMIAVEITLSKWLFNAVQAHEVLTINPDY
                     FRLRRPIERRLYELARKHCGEQPRFAIGIELLQEKCGSQAALKEFRRALRELVDADTL
                     PDYKIELDDDKGQAVFFKREVLRTSSVSG"
BASE COUNT         1476 a         2745 c         2446 g         1359 t
ORIGIN      
        1 aagaccgctg cataacctgc tgatgagcac gtgagggtca gggctgctga atctgagcag
       61 tgttttcggt gccgtctggc ccggtatttt gttttgaggc tgcgtttgca gccccgtttg
      121 ccccactcag ggcgcacctg ctcccgcaag ctccattcgt ttgagcttgc ggtgagcctt
      181 cagcaggttc atacccagcg ccgcccagca catctgcgcc tgcaccttgg ccgtgccgaa
      241 gtaccgcgct cgggccaagt ggaatcggcg cttcatcgtg ccaaaggcct gctcgacctt
      301 gaaacgaatg cccccgatct tctggttgaa ggctttgagc agcggatgca cgggcttgcc
      361 ggcgctgcct ttgtgctgga tcagatcgcc catgccgcgc tcttggagcc attgccggtt
      421 ggccttgctg gcatagccct tgtcggccag aacggcttca accccgggcg agagtgcttc
      481 gacgatctca ggcagtttgt tgatctcggc ctcgttggcc ggatgcactt gcacatgctc
      541 gacgtagccg tcttcactgt ccacggccgt gtgacctcgg tagccaaaga aggcttgttt
      601 acccttcttg acccagcggg catcggcatc ggcactgtcg acgatcctgg cttgctcgcc
      661 ttcgccttcc acatgctggc gcggccgtgc ggcgctgggg atgatggtgg catcgatgat
      721 ggccccgcgt gcgccctgta ccttcaagcc acgctgctcc agttggctgt tgatgagcgt
      781 cagcagcttc tgatccaact ccgccttgac caagcggttg cggaagcggc agatggtgct
      841 ggcgtcgggc aactcacctg ccgagggctc gaagccggtg aagaccatga agtccagccg
      901 gacgcgcagt gcttgctcca gttgggcatc cgacagacca tgccactggc ccagcaacat
      961 cagcttgaac atgcccagcg gtgagtaggg ctcgggaccg ccagcgccgg agatctcgcg
     1021 ctggtacagg cctttcaact gagcagcaat ggcaccccaa tcgagcaatt cgtgcagctt
     1081 catgctcggg ctgccttcga ccagcgggcg cgcgcccatc agaaagaagc tgtccatcgc
     1141 caccatcacc tcctggcacc attgcagcgc agatcggacg agtgcgcatc gggactgcta
     1201 ccagttatgc agcggtcttg ataggtaacc aaatcatcta atattgtcag gagcgtaaga
     1261 tgtaattggt ggtgtgtgag cattgccatt gcgttaacga gtccgggctt cgcggtgccc
     1321 tctttgggga ggggggggcg aaggctcggg agaattaccg aggggaaccc tgtcatgagc
     1381 attcgtttgt tttcagaggc gtgcactgcg ccgtcggcga gtccttcagc ccggcccgcg
     1441 ctgcgccgtg gtttctgggc tgcgctggta gccgctccgt tgttgtgcgc ggcgggcggt
     1501 gcctgggcgc agagcgcgga cctggtgctc gccaaccacg tcgtcacgcc cgacccggtg
     1561 ccggccggcg gtatcgccac catcaccatg acggtgcaga acaacggcac cggcgccgcc
     1621 agcaacgtcg tgctgaccga caccattccg gccggctcca ccttcgtgga catgagtgct
     1681 agcgacggcg gcacctgcac cgccagcgcg ccctaccact gcgactgggc ttccattccc
     1741 ttccccggct cgcgcacggt cacgctgcgc gtgcggctgc ccagcgcaca ggtgtggacg
     1801 aatgcggcca cggtcacctc cagcaccgcc gacccgaacc aaggcaacaa cagcctgagc
     1861 cgcaccatca ccgtgcagca ggcggccgac ctggccgtca ccgccaccag ttcggccggc
     1921 ggcaccattg ccgcgggcac accctacacc tacacgctga acgtcgccaa caacggcccc
     1981 gacccgttgc cggccggcca gtcgcccacc gtcaccttca acgtgccgtc aggctcgtcc
     2041 atcacctcgc ggcctggcgg aacgggctgg gactgccagc ccgccagcgg ctacccgctg
     2101 accgatgagc cgggcccgcc cgtcggcgcc cagatcacct gcacccgcag cgacggtctg
     2161 gccaatggca gcagcttccc gcccatcacc gtgaacgccg tcggcaacgt caccggcgcc
     2221 gtcgacgcca cgttcgacgt ctcgtccaac tacctggacg gcaacaccac caacaacacc
     2281 gccacggtga gccagccgct gagcgccggc accgacatga gcatccgcaa gaccgtggcc
     2341 ctggccgcgg gcaccagcgg cacccaggcc accttcaccc tgcgcgcgcg ccaggaaggc
     2401 ggcgccgcgc ccaccggcgt ggtcgtgacc gacacgctgc cggccggcat gacctacgtg
     2461 agccacaacg cgccggcgcc ctggaactgc acctgggcgg caccgctgct gacctgcacc
     2521 taccccggca cctacaccgg cggcgcgttc accgacctgc ctgacatcca actggtggcc
     2581 gacgtcaccg ccaccggcac catccccaac acgggccacg tgacggccac gccgcccgac
     2641 ccgaacggca ccaacaacag cagcaccgtc gaagtcacca acacggccga cctgcgcatc
     2701 accaaatcgg ccagcctcag cccggtggtg acgggccagc cttacaactg gaacatcgtg
     2761 gtcgccaact acggccccat gcccgtgctg gcgggccagt acatcacagt gacggagacc
     2821 attcccgccg gcatgacgat aaacagcgcc agcggcactg gctgggcgtg ctattccgac
     2881 gctggctatg ccacggcagc ggtgtacccg gtagccggtg gcgccaccct ctactgccgg
     2941 caactgcgca cgtccaacct gggattcaac gccaccaacg gcaccgctac gaatacgctg
     3001 acgctggcag cagtcaacac cgtcgccggt tcgctgaaca acagcgcctg tctgggcttg
     3061 agcggcaacg gccccaacga agccggtggc gcgcccgact tccagcgcaa ctgccacggc
     3121 gtcggcacca acggcaccct gcccgagaac tccgccgacc tggagatcgt gaagaccgtg
     3181 gtgggcccca accccgtggt ggtgggccag cccctgacct accgcctggt ggcccggaac
     3241 ctgtccacgt ccgtcaacgc gacccaggtg cacgtgtacg acaccgtcaa caacctgcgg
     3301 aacatcgccg gctttccggg cttggtgtcc gtgacgacga cgcagggcac ctgcgcgccc
     3361 gccacgccgg ccagcgtcac ctcggccagc atcgactgca acctgggcac gctgaacgcg
     3421 ggcgcctccg ccacgatcga catcacgatc cgtcccaaca acaccacggc gacggacctg
     3481 acgcgcggca acacggccac cgtcaattcg ctggacgtgg gtgaccccaa ccgcgccaac
     3541 aacacctcga ccgtttccag cgtggtgcag ccgcgtgtgg acatgacggt cagcaagtcc
     3601 gtcaacccca ccccggtgcg cgtgggccag cccatgacgt acacggtcac ggccaacaac
     3661 gccgggccgt cggaggcgac caacgtccgc atcaccgacg tgatgccgcc caacaccgcc
     3721 ttcatcagcg tcggcacgcc gtcgaacggc ggcacctgcg gcaccgtgcc tgcggccgat
     3781 tccaccggcg gcacgctgga gtgcacctgg gcctcggtgc ccgcgggcgg caaccgtacc
     3841 gtgaccttca ccgtccgtcc gctcgaggcg gcgctgggcg gcggcaccac cggcaccatc
     3901 aacaacacgg tcaccgtggc caccgacagc acggaaacca acctggacaa caactcggcc
     3961 agcatcccgg ccgaagtgat cgcgtccctg gtggacatcc tggtgcagaa gaccgacagc
     4021 gtggatccgg tggtgctggg cgcgacgacc cgctacacca tcaccatccg caacgccggg
     4081 ccgtcctacg gcaccaacct ggtgatgacc gacactttcc ccaacgccgg caacaccgcg
     4141 cgcttcagct accagggcgg cctgagggcc accgccgcgg gcaccgctgt cacgccggtg
     4201 tgcacggagc cggccatcgg cgccatcagc ggcacgctga cctgcacctt cccgaccatc
     4261 gccgtcggcg cggccaacga agtggtgctg caatacgaca tgcgggccga gagcatcgtc
     4321 accgccggcg actactcggg cacgcagggc aaccacgttg ccgtggcggt ggccgagaac
     4381 gagaccgaga tgggcaacaa ccaggtcgat gaacacacca ccacccgccg cgaggccgtt
     4441 cccaccgacc tgggcctgac caaggcagtg gacaaggcca cgatggccgc tggggagcag
     4501 gtggtctata ccctgacggt gcgcaacaac ggcccgcagg aaagccgcgg cgcccaggtc
     4561 atcgacacgc tgcccgccgg catgagcttc gtcgcgtcgc ccgacggctg cgtgaacagc
     4621 gccggcacgg tgacctgcgc cgtgggcacg ctgcccgtgg gcgagacccg cgtcttcacc
     4681 ttcacggcgc aactggccga cccgtacaac ggccccgccg cgctggtcaa caacgcgcgc
     4741 ctggacgcgc ccggcgacat cgatctgagc aacaacgagg acgaggtcac cacccgcgtg
     4801 cctccgccca gcacgcccat cccgacgctg tcgcagtggg gcctgatcct gctgtcgctg
     4861 ctgatggccg gcttcgccct gcgccgccgc gtgtaagcgc ctcgccgcac agacgccaag
     4921 gccgcatcgc cgccgccgcg cccgccaggg cgttgcggcg gcgttttttt gggaccgcgg
     4981 ctggggcaga atggctgtta gcaatattgg cagaatcgac ctctagccta tatgagacaa
     5041 gcgcaagcag ctatccaagt aatagcatga atccttccga catcctcctg ctgcccggct
     5101 ggcaggactc cggccctggc cactggcaga cggcctggga gcagcggcac ggctgcacgc
     5161 gcgtggcgca gcacgactgg atgcgcccgc tgcgcggcga ctggacggcg cggctgcagg
     5221 acgtggtgct ggcccgcccc ggcccggtgg tgctggccgc gcacagcctg ggctgcctgc
     5281 tggccgcgtg gtaagaccgt tgcataaccc acccatgagc gcgcaaggtt gttgttgctg
     5341 aatctgagta gcgttttcga tcccggccgg ccggctcttt tgtctcgggg ctgcgtttgc
     5401 agccccgttt gccccactca gggcgcacct gctcccgcaa gctccattcg tttgagcttg
     5461 cggtgagcct tcagcaggtt catacccagc agcgcgtgag ccgtgcgcaa atcgtccccc
     5521 cacgtctgct gtgcagttcg ctgccccgtc aaccaggtac gcgccttgcc cagcgccgcc
     5581 tgcaccgccg gctgctgccg atacaaagtc aaggcttgaa cggccaatgc cgtggtcacc
     5641 acggcatcgc catcgggcac caacttccac tgcccgtccg cctgctgcgc cgagagaagc
     5701 cagcccagcg ccttcactgc cgcggcgctg ctggcttggt tggccgccag cgcccgcaac
     5761 gcccacgccg tatcctgagc gttgctcgcc agtccggcgg ccgcgccaaa ccccccatcg
     5821 gcattctgga gtttgatcag tgcatcgagg tgcgtggcat ccgagactgc tgcgagttgc
     5881 ttgccaatcg ccttgcgtgc caggtactcg gtggtgtccg gggtagcccc ctcaatcgcc
     5941 gtgtacagcg ctggaggagc cgtttgcgcc aagcgccgca gcgtgatcgc ggcctcggaa
     6001 cggacctgca ttggcaaggc gggtgaaccc gcttccgaag ccagttgccc agaggcctga
     6061 atctgtccct gcaaccaggc cacgccccgt tgcaccgccg gatcggtcaa accttgggaa
     6121 aaggccgagc ctgcaagaaa cagccagctc acgcacagcc atcggagcca cacgatcaat
     6181 cgatggccaa gcgcagacgg ccatgcagca acttgcattg aggtaccctc ctccgggcga
     6241 ttttggtctg gatcgtaacc cagacgtacc aatcgttaac ttataccccc atgagaacaa
     6301 aaatgtaagt aaccagaaag ccccctttcc tgttaaacag gtagcagggg ggagccgcga
     6361 agcgggggga gtgcagctcc cccgaggggg tcgcgcccgg ccgtgaaggg ccgtaaggcc
     6421 cgcaacaggc ggcaggggga gcgtagctcc ccgcgggcca gggggacgtg atgaatcccc
     6481 cgcttgcggg ggacgattga aagggtgagc gcaaagcgcg agggccaagc cctgggcgtt
     6541 ggtattggct gttgggggat gccgggaggg agcgcgaagc gcgggcgaag gcccgaaggg
     6601 ccgtaggagc cggcgggggc gtagccccca agaggcggtg ctgctcggac ggctgacgtt
     6661 aaataaaaaa gaaagttaaa gagcgcgcgc gcgtagttaa ggctgtggaa aacgttcgca
     6721 actcactgat gcaccagact ttttttccat acttggtggg tgatataacg agagggggta
     6781 gcgacttggt gggtgatgta acgagtcaat ccacagacgg ggtgggcgat gtaacgagtc
     6841 aatccacagc ggtgggtgat gtaacgatag cacccatcaa gagaatttag gcactttagt
     6901 attaacaaag tactggggcg acccaacttt agcctgatgt cgcatcattt gatgtgatgt
     6961 gacatcacta atttcaagtc agttggcagt ctggcgacgc aaccggtggg tgatataacg
     7021 agtctcgtta cccagagacg gaactcgttc gaaggacctc gcgcttgaag aagacagcct
     7081 gccccttgtc atcgtccagc tcgatcttgt aatcaggcag cgtgtccgca tccaccagct
     7141 cccgaagggc gcgcctgaac tccttgaggg cagcctggct gccgcacttt tcctgaagca
     7201 gctcgattcc aatggcgaaa cgaggctgtt ctccgcagtg ctttctggcc agttcgtaga
     7261 gccgccgctc tatggggcga cgaaggcgga aatagtcggg attgatggtc aagacctcat
     7321 gggcttggac cgcgttaaag agccacttgg agagtgtgat ctcgacggcg atcatctgac
     7381 tgtcgtcggg tgacttctcg atgatcgtcc agctatcgac gattccgaac ccttccttga
     7441 cgcgctggcc acccgtcctg atgttggtgg tgatcgaggt gccgcgtagc cgctctagag
     7501 cgtcttcaag gcgttggtac tccttacctc cagtcagctt gttggttgac actaggtagt
     7561 catacacgcg aaacctgacc gtccggttgt tggcatcggg ccgcccacga ttgagcgcat
     7621 ccgtcatctg gctgactacg tagatcagca agtctttgtc aaactgagtc gccctgccct
     7681 tcacgctcgg tgttacttcc accgatctgt ttccgtcacg gctttgccag cgccagattt
     7741 ttgtgtcggg cttcgtcgcc aaggtgaaga taggcgcttc catgctgacg ccgtcgtcct
     7801 tgagggcata gtcgaacagg tcgcagagga aaaagtctcg gttggggtga tggataggca
     7861 tcaacccacg accaagacct cgcgccttct cgccctgccc ttgatcagcg tcttccgaag
     7921 acgcttcccc tatctctttg ctcacgactt ggcctagcaa atcgccgatg ggggggtctc
     7981 ctcgttttca gtgcaataag tgacggtacg caaagctagc actggc
//
LOCUS       DAOOOG010000123         7933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_10721_length_7933_cov_34.977532, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000123 DAOOOG010000000
VERSION     DAOOOG010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10721_length_7933_cov_34.977532"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PK242_09490"
     CDS             complement(<1..636)
                     /locus_tag="PK242_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOZ94291.1"
                     /translation="MATPIDIDFVSDIACPWCAVGLASLQQALARCADAVQVRLRVQP
                     FELNPAMPPEGEDLGEHLTRKYGSTPAQQAQMRAVIAERGAALGFAFHPEGRGRVVNT
                     FDGHRLLHWAGRQGADAQLALKRALLQAYHGRAERVDQAPVLLAAVRQAGLDEARAAQ
                     ILAGDEFAAEVRQAERQWQDAGIHAVPAVILNQRHLISGGQPPEVFEQALRQ"
     gene            complement(827..1756)
                     /locus_tag="PK242_09495"
     CDS             complement(827..1756)
                     /locus_tag="PK242_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOZ94292.1"
                     /translation="MNRTPDPGPGQRAASAGPTPLDAAAFPAALPPAERLRLASGQRL
                     TTPGGQGELVWHRWGTRRAGQPPVVLLHGGSGSWTHWVKNIAPLLDAGRQVLAADLPG
                     FGDSAPPTQGSDADAMVEPLANGLRQLQPGEAVDLVGFSLGGLTAALLLAEHPALARR
                     LVLVGAPAMGVVPGRQFELRAWRHLPSPAAQAEVHRHNLAVLMLHDPGLIDGLALQLH
                     IANVQRDRLPRRRLAHTDILARRLPQVACPVHAIYGVHDAIYRQHIHALEGAFARSAP
                     DFRGLQLIEGAGHWVQFEAPAAFDAALRRALGD"
     gene            complement(1753..2178)
                     /locus_tag="PK242_09500"
     CDS             complement(1753..2178)
                     /locus_tag="PK242_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOZ94293.1"
                     /translation="MADPMERQRIDKWLWAARFYKTRALAVQEIERGRVQVAGQTVKP
                     ARELRPGDVLSLRQGVVQREVVVRALSPQRGPAPVAQQLYEETPASVAARQQAASARR
                     LAPEPALSIAQGRPTKRDRRALDQQQRADWGERWSAGIE"
     gene            2237..4777
                     /locus_tag="PK242_09505"
     CDS             2237..4777
                     /locus_tag="PK242_09505"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOZ94294.1"
                     /translation="MPQPSHPPGLSGDEARRRLAEHGPNTIDERERRSLAGTLREIAR
                     EPMFLLLLVAAAIYLLVGEPGEGLLLGGFALLTVGLVIFQQRRGERALDALRELAAPQ
                     AQVLREGRVQRIAVREIVPGDWIVLGEGERVAADAVLRQAQALTVDESLLTGESVPVR
                     KCASHAPLVEAATDRPGGDDLPQVFAGTLITAGHGLAEVCATGRQTQMGRIGAALATI
                     EPAATPLQRSLHRLARWFGALALTGSALLVLWHGLRGGDWVQGLLSGIALGMAMLPEE
                     FPMALAVFLALGSWRLARIQVLARQPAVVEALGATTLLCVDKTGTLTENRMALRRLVT
                     QEVDVTVQAGAPLPASVHRLLACAMLASRRGTSDPMDRALLLHGDQALAGTEHLHPDW
                     QLEREYALTPALPAMTQTWLDDAGRRHVAAKGAPEAVIELCHLPPAAAARLLAQVAAL
                     AAAGLRVLAVAEGQGSTAETAPDPHDYDLRLLGLLGFEDPLRASVPAAVAQAHGAGIA
                     VAMITGDHAATALAIARQAGIDTAAGALTGAQLAALDDAALSAAVRRVRVYARVLPEQ
                     KLRLVEAMQRNGETVAMTGDGVNDAPALKAAHIGIAMGGRGTDVAREAAGLVLLDEDF
                     SRIVGGVRMGRRIFDNLRKAMVYIAAIHVPIAGLALLPVLLGLPPLLLPAHVVLTEMV
                     IDPICSLAFEGAPEDPRLMQRPPRRAAEGLVGRSLLAQAAVQGASVLAATLAIYLQAL
                     HGGQSADVARTLAVIGLMAGNLLLVAVNLSAGLGWRALLARGARAFWAVAAIATAVLG
                     LALALPQARALLRFGPVSLAQVGLALLAVALAVVVGARFGRALERSTA"
     gene            4819..6324
                     /locus_tag="PK242_09510"
     CDS             4819..6324
                     /locus_tag="PK242_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOZ94295.1"
                     /translation="MPATLSPAPSALPRDASRILELAARSLFELFEDASEGMLLVDRD
                     ARVVWINDPYRRFLPALGFEREEDFVGHPVSDVVQNTQMHQVLRTGKPILIDLLSNRA
                     GTFAVSRIPLRDAAGEVIGALGIVLFDQTSHSLQPLLAKFARLQQELDDAQRELAAQR
                     RQQGDGQRRARYTLASFVGSSPAASEVKRQARRAAQSSSPVLLLGETGTGKELLAHGI
                     HAASPRAGGPFVSVNIAAVPDTLLEAEFFGVAPGAYTGADRKGREGKFRLADGGTLFL
                     DEIGDMPPGLQAKLLRALQDGEIEPLGSNRLVPFDARIIAATSRDLPQLVRAGQFRED
                     LYYRLNVLPIQVPPLRTRRGDIPALIEVLAEDMLHRGSQPPELTPAAVALLAAQHWRG
                     NIRELRNVLEQAALRADAGALDAEPMEALLRQSGLSLVQPALPPEPPPQDAAALLRPL
                     GEQVAELERRAIAAALQATHGNKLAAARLLGISRATLYGRLLEPGDAPQKP"
     gene            6487..7674
                     /locus_tag="PK242_09515"
     CDS             6487..7674
                     /locus_tag="PK242_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOZ94296.1"
                     /translation="MQRRSLIALASFAVLTGAPAFAAGDIKIAHVYSKTGPLEAYGKQ
                     TQTGLMMGLQYATGGTMQVNGRKITVIERDDQGKPDLGKSLLAAAYADDKVDLAVGPT
                     SSGVALAMLPVAEEYKKILLVEPAVADAITGDKWNKYIFRTGRNSSQDAISNAVAMDK
                     DGVSVATLAQDYAFGRDGVKAFKDALKKARIVHEEYLPQNTTDFTAGIQRLIDALKDK
                     PGRKAIEVIWAGATPPFNALAAADLKKRYGIEVFTGGNILPAMASYKNFPGMEGATYY
                     YFGIPKNPVNEALVAAHYKEFKAPPDFFTAGGFSAAMAIVTALKATGGDTATNKLIKT
                     MEGMSFDTPKGKMTFRKDDHQAMQSMYHFRIKADPAFTWGVPELVKEIKPEEMQVPIR
                     NKR"
     gene            7804..>7933
                     /locus_tag="PK242_09520"
     CDS             7804..>7933
                     /locus_tag="PK242_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ94297.1"
                     /translation="MLETEDLTIRFGGHVAVNAVTCRFEPGTLTAIVGPNGAGKTTY"
BASE COUNT         1142 a         2892 c         2829 g         1070 t
ORIGIN      
        1 ctgacgcagc gcctgctcga acacctcggg cggctggccg ccgctgatca ggtggcgctg
       61 gttgaggatc accgccggca ccgcatggat gccggcgtcc tgccactggc gctcggcctg
      121 gcgcacctcg gcggcaaact cgtcgcccgc cagaatctgc gccgcccgcg cctcgtccag
      181 ccccgcctgg cgcacggcgg ccagcaggac cggggcctgg tcgacccgct cggcgcgtcc
      241 atggtaggcc tgcagcagcg cgcgcttgag cgccagctgg gcgtccgccc cctgccgacc
      301 ggcccagtgc agcaggcggt gcccatcgaa ggtgttgacg acgcgcccgc gcccttcggg
      361 gtggaaggca aagcccagcg ccgcgccgcg ctcggcgatg acggcgcgca tctgcgcctg
      421 ctgcgccggc gtggagccgt acttgcgagt caggtgctcg ccgaggtcct caccctcggg
      481 cggcatggcg gggttgagct cgaaaggctg aacacgcagc ctcacctgca ccgcgtcggc
      541 gcagcgcgcc agcgcctgct gcagcgacgc caggccgacc gcgcaccagg gacaggcgat
      601 gtcggagacg aagtcgatgt cgatcggtgt ggccatgcaa accctccaag tgctactaac
      661 taaatagctg ccggcgcttt gccggtgcgc gtcagggcca tgaatcgctt aaatccgacc
      721 gctggcccat tcgtgcgcca gcgctgcgcc tgtggcgcgt cggtggtgcc ggcttcgcgt
      781 ccgcgacgtc cttcacgtcc ggctgccgga gcgtgccggc aggccatcaa tcccccagcg
      841 cgcgccgcag cgccgcgtcg aaggcggcgg gcgcctcgaa ctgcacccag tggccggcgc
      901 cctcgatcag ctgcaggcca cggaagtcgg gcgcgctgcg cgcgaacgcg ccctccagcg
      961 cgtggatgtg ctggcgatag atggcatcgt gcacgccgta gatggcatgc accgggcagg
     1021 ccacctgcgg caggcggcgc gccaggatgt cggtatgcgc cagccgccgg cgcggcaggc
     1081 ggtcgcgctg cacgttggcg atgtgcagtt gcagcgccag gccgtcgatc aggcccggat
     1141 cgtgcagcat cagcaccgcc aggttgtggc gatgcacctc ggcctgcgcc gccggcgacg
     1201 gcaggtggcg ccaggcgcgc agctcgaact gccgcccggg caccacgccc atggccggcg
     1261 cccccaccag caccagcctg cgcgccagcg ccgggtgttc ggccagcagc agggcggcgg
     1321 tcaggccgcc gagcgaaaag cccaccaggt cgaccgcctc gcccggctgc agctgccgca
     1381 ggccgttggc cagcggctcg accatggcat cggcatcgct gccctgcgtg ggtggggccg
     1441 aatcgccaaa gcccggcagg tcggccgcca gcacctgacg cccggcatcg agcagcggcg
     1501 cgatgttctt cacccagtgg gtccatgagc cgctgccgcc atgcaacagc accaccggcg
     1561 gctggcccgc gcgccgcgta ccccagcggt gccagaccag ctcgccctgc ccgcccggcg
     1621 tggtcaggcg ctggccgctg gccaggcgca agcgctcggc cggcggcagc gcggcgggga
     1681 aggcggcggc gtccagcggg gtcgggccgg cgctcgcggc gcgctggcct gggcctgggt
     1741 caggggtgcg gttcattcga tgcccgccga ccagcgttcg ccccagtcgg cgcgctgctg
     1801 ctggtccagc gcacgccggt cgcgcttggt gggccggccc tgggcgatgc tcagcgccgg
     1861 ctcgggcgcc agacggcgcg cgctggcggc ctgctggcgc gcggcgacgc tggccggcgt
     1921 ttcctcgtac agctgctgcg ccaccggggc cgggccgcgc tgagggctga gcgccctgac
     1981 caccacctcg cgctgcacca ccccctggcg cagactgagc acatcacccg gcctgagctc
     2041 gcgcgccggc ttgaccgtct gccccgccac ctgcacgcgg ccgcgctcga tctcctgcac
     2101 cgccagcgcg cgtgtcttgt agaagcgcgc ggcccacagc catttgtcga tgcgttgtcg
     2161 ttccatgggg tcggccatgg gctcgattct gcccggcttt gcctgcggcg gacgcgggcg
     2221 gggatactca cgggtgatgc cacaaccctc ccatcccccg ggcctgagcg gcgacgaagc
     2281 gcgccggcgc ctggccgagc atgggcccaa caccatcgac gagcgcgagc ggcgcagcct
     2341 ggctggcacg ctgcgcgaga ttgcgcgcga gccgatgttt ctgttgctgc tggtggcggc
     2401 ggccatctac ctgctggtgg gcgagccggg cgaaggcctg ctgctgggcg gctttgcgct
     2461 gctgacggtc ggcctggtga tcttccagca gcggcgcggc gagcgcgcgc tggacgcgtt
     2521 gcgcgagctg gcggcgccgc aggcccaggt gctgcgcgag ggccgcgtgc agcgcatcgc
     2581 ggtgcgtgag atcgtgcccg gcgactggat cgtgctgggc gaaggcgagc gcgtggccgc
     2641 cgacgccgtg ctgcgccagg cccaggcgct gacggtggac gagtcgctgc tgaccggcga
     2701 atcggtgccg gtgcgcaagt gcgccagcca cgctccgctg gtcgaggcgg cgaccgatcg
     2761 gcccggcggc gacgatctgc cgcaggtgtt tgccggcacc ctgatcaccg ccggccacgg
     2821 cctggccgag gtctgcgcca ccggtcgcca gacgcagatg ggacgcatcg gtgcggcgct
     2881 ggcgacgatc gagccggccg ccacgccgct gcagcgcagc ctgcaccgac tggcgcgctg
     2941 gttcggcgcg ctggcgctga ccggcagcgc gctgctggtg ctgtggcacg ggctgcgggg
     3001 gggcgactgg gtgcagggcc tgctgtccgg catcgcactg ggcatggcca tgctgccgga
     3061 agagttcccg atggcgctgg cggtgttcct ggcgctgggc agctggcgac tggcgcgcat
     3121 ccaggtgctg gcgcgccagc ccgccgtggt cgaggcgctg ggggccacca ccctgctgtg
     3181 tgtcgacaag accggcaccc tgaccgaaaa ccgcatggcg ctgcgccggc tggtgacgca
     3241 ggaggtcgac gtcacggtgc aggccggcgc gcccctgccc gcgtcggtgc accggctgct
     3301 ggcatgcgcc atgctggcgt cgcggcgcgg caccagcgac ccgatggatc gcgccctgct
     3361 gctgcacggc gaccaggcgc tggccggcac cgagcacctg caccccgact ggcagctgga
     3421 gcgcgagtac gccctcaccc cggcactgcc cgccatgacg cagacctggc tggacgacgc
     3481 cggtcggcgc catgtggccg ccaagggcgc gcccgaggcg gtgatcgagc tgtgccacct
     3541 gccacccgcg gcggcggccc gcctgctggc gcaggtggcg gcgctggccg ccgccggctt
     3601 gcgcgtgctg gccgtggccg agggccaggg cagcacggcc gagaccgcgc ccgacccgca
     3661 cgactacgac ctgcgcctgc tcgggctgct ggggttcgag gacccgctgc gtgccagcgt
     3721 gccggcggcg gtggcgcagg cgcacggcgc cggcatcgcc gtggccatga tcaccggcga
     3781 ccacgccgcc acggcgctgg cgatcgcacg ccaggccggc atcgacacgg cggccggcgc
     3841 gctcaccggc gcgcagctcg ccgccctcga cgatgccgcg ctgtcggccg cggtgcgccg
     3901 ggtgcgcgtg tacgcgcgcg tgctgcccga gcagaagctg cgcctggtcg aggccatgca
     3961 gcgcaacggc gagaccgtgg ccatgaccgg cgacggcgtc aacgacgcgc cggcgctgaa
     4021 ggccgcgcac atcggcatcg ccatgggcgg gcgcggcacc gacgtggcgc gcgaagcggc
     4081 cggcctggtg ctgctggacg aagacttcag ccgcatcgtc ggcggcgtgc gcatgggccg
     4141 gcgcatcttc gacaacctgc gcaaggcgat ggtctacatc gccgccatcc acgtgccgat
     4201 cgccggcttg gcgctgctgc cggtgttgct gggcctgccg ccgctgttgc tgccggcgca
     4261 cgtggtgctg accgagatgg tgatcgaccc gatctgctca ctggcgttcg agggcgcgcc
     4321 ggaagacccg cgcctgatgc agcgcccacc gcgccgggcc gccgaaggcc tggtcggccg
     4381 gtcgctgctg gcgcaggcgg cggtgcaggg tgcgagcgtg ctggccgcca cgctggcgat
     4441 ctacctgcag gcgctgcacg gcggccagag cgccgacgtg gcgcgcacgc tggccgtgat
     4501 cggcctgatg gcgggcaacc tgctgctggt ggccgtcaac ctgagcgcgg gcctgggctg
     4561 gcgcgccctg ctggcccgcg gcgcgcgcgc cttctgggcc gtggcggcca tcgccacggc
     4621 ggtgctgggc ctggcgctgg cgctgccgca ggcgcgcgcg ctgctgcgct tcgggccggt
     4681 gtcgctggca caggtcggcc tggcgctgct tgccgtggcg ctggccgtgg tcgtgggcgc
     4741 gcggttcggc cgggcgctgg agcgcagcac ggcctgacgg cgccgcgcgc gcttcggccc
     4801 gcgcgctacc atgcgcgcat gcctgccacg ctgtcgcccg ccccgtccgc actgccgcgc
     4861 gacgccagcc gcatcctgga gctggcggcg cgctcgctgt tcgagctgtt cgaagacgcc
     4921 agcgaaggca tgctgctggt cgaccgcgac gcccgtgtgg tgtggatcaa cgacccctac
     4981 cggcgctttt tgccggcact cggattcgag cgcgaggaag acttcgtcgg ccacccggtg
     5041 tcggacgtgg tgcagaacac ccagatgcac caggtgctgc gcaccggcaa gccgatcctg
     5101 atcgacctgc tgagcaaccg cgccggcacc ttcgccgtca gccgcatccc gttgcgcgac
     5161 gcggcgggcg aagtcatcgg cgcgctcggc atcgtgctgt tcgaccagac ctcgcacagt
     5221 ctgcagcccc tgctggcgaa gttcgcgcgc ctgcagcagg aactcgacga cgcccagcgc
     5281 gagctggcgg cgcagcgccg ccagcagggc gatggccagc gccgcgcccg ctacacgctg
     5341 gccagcttcg tcggcagcag cccggcggcc agcgaggtca agcggcaggc gcgccgcgcc
     5401 gcgcagtcca gcagcccggt gctgctgctg ggcgagaccg gcaccggcaa ggaactgctg
     5461 gcgcacggca tccacgccgc ctcgccgcgc gccggcgggc cgttcgtcag cgtcaatatc
     5521 gccgccgtgc ccgacacctt gctggaagcc gagttcttcg gcgtcgcccc cggcgcctac
     5581 accggcgccg accgcaaggg gcgcgaaggc aagttcaggc tggccgacgg cggcacgctg
     5641 tttctcgacg agatcggcga catgccgccc ggcctgcagg ccaagctgct gcgcgcgctg
     5701 caggatggcg agatcgaacc gctgggcagc aaccggctgg tgccgttcga cgcccgcatc
     5761 atcgccgcca ccagccgcga cctgccgcag ctggtgcggg ccggccagtt tcgcgaggac
     5821 ctgtactacc gcctgaacgt actgccgatc caggtgccgc cgctgcgcac gcggcgcggc
     5881 gacattccgg cgttgatcga ggtgctggcc gaagacatgc tgcaccgcgg cagccagccg
     5941 ccggagctga ccccggccgc cgtggcgctg ctggcggccc agcactggcg cggcaacatc
     6001 cgggaactgc gcaatgtgct ggagcaggcc gcgctgcgcg ccgacgccgg ggcgctcgac
     6061 gccgaaccga tggaagcgct gctgcgccag tccggcctga gcctggtgca acccgccctg
     6121 ccgcccgagc cgccacccca ggacgccgcc gcgctgctgc gtccgctggg cgaacaggtg
     6181 gccgaactgg agcgccgcgc cattgcggcc gcgctgcagg ccacccacgg caacaagctg
     6241 gccgcggcgc ggctgctggg catctcgcgc gccacgctgt acgggcgcct gctcgaaccc
     6301 ggcgacgctc cgcagaaacc ctgatctaaa ttcagtcatt tgtctgaata tcagacaaag
     6361 aatttgtcca tgattcagac actttgaagc ttgcggccaa gcagagcgcc atgaatcaat
     6421 gacttggcag ctggcacggc ttgtgcatgg tggatggtga catcgtcacg tccacaggag
     6481 accctcatgc aacgccgctc gctcattgcc ctcgcttcgt tcgccgtgct gaccggtgcg
     6541 ccggccttcg ccgccggcga catcaagatc gcccacgtct acagcaagac cggcccgctg
     6601 gaagcctacg gcaagcagac ccagaccggc ctgatgatgg gcctgcagta cgccaccggc
     6661 ggcaccatgc aggtgaacgg caggaagatc accgtgatcg agcgcgacga ccagggcaag
     6721 cccgacctgg gcaagagcct gctggccgcc gcctacgccg acgacaaggt ggacctggcg
     6781 gtcggcccca ccagctcggg cgtggcgctg gccatgctgc cggtggccga ggaatacaag
     6841 aagatcctgc tggtcgagcc cgccgtggcc gacgccatca ccggcgacaa gtggaacaag
     6901 tacatcttcc gcaccggccg caacagcagc caggacgcca tcagcaacgc cgtggccatg
     6961 gacaaggacg gtgtttcggt ggccacgctg gcgcaggact acgccttcgg ccgcgacggc
     7021 gtgaaggcgt tcaaggacgc cctgaaaaaa gccaggatcg tgcacgagga atacctgccg
     7081 cagaacacca ccgacttcac cgccggcatc cagcgcctga tcgacgccct gaaggacaag
     7141 cccggccgca aggcgatcga ggtgatctgg gccggcgcca cgccgccctt caacgccctg
     7201 gccgcggccg acttgaaaaa gcgctacggc atcgaggtgt tcaccggcgg caacatcctg
     7261 ccggccatgg ccagctacaa gaacttcccc ggcatggaag gcgccaccta ctactacttc
     7321 ggcatcccca agaacccggt gaacgaagcc ctggtggcgg cgcactacaa ggaattcaaa
     7381 gcgccgcccg acttcttcac ggccggcggc ttctccgccg ccatggccat cgtcaccgcg
     7441 ctcaaggcca ccggtggcga caccgcgacc aacaagctca tcaagaccat ggaaggcatg
     7501 agcttcgaca cgcccaaggg caagatgacc ttccgcaagg acgaccacca ggccatgcag
     7561 agcatgtacc acttccgcat caaggccgat ccggccttca cctggggcgt gcccgagctg
     7621 gtgaaggaaa tcaagccgga agaaatgcag gtgccgatcc gcaacaagcg ctgatggcgt
     7681 cgatggcgca tgacgcatga cgcatgacgg gcgtgtcgtc ggtgcgatgc gcccggcacc
     7741 ccgccatggg tcatccatgc cgcgccagcg gcgaggtcat atcgtcattg gcaacacacc
     7801 gacatgctag agaccgaaga tctgacgatc cgctttggcg gccatgtggc cgtcaacgcc
     7861 gtgacgtgcc gcttcgaacc cggcacgctg acggccatcg tcgggcccaa tggcgcgggc
     7921 aagaccacct act
//
LOCUS       DAOOOG010000124         7811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_10961_length_7811_cov_26.390021, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000124 DAOOOG010000000
VERSION     DAOOOG010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10961_length_7811_cov_26.390021"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PK242_09525"
     CDS             complement(<1..904)
                     /locus_tag="PK242_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HOZ94298.1"
                     /translation="MKNEWFSKIAIWMVIAMVLFTVFKQFDGGRMASANHMAYSDFLE
                     EVRNNRVKQATIQEGQGGTDIVAVMSDDRRVRTQATYLDRGLVGDLINHNVKFDVKPR
                     EEGSLLMTLLVSWGPMLLLIGVWIYFMRQMQGGGKGGAFSFGKSKARMLDENNNTITF
                     ADVAGCDEAKEEVKEVVDFLKDPQKFQKLGGRIPRGLLLVGPPGTGKTLLAKSIAGEA
                     KVPFFSISGSDFVEMFVGVGAARVRDMFENAKKNAPCIIFIDEIDAVGRQRGAGLGGG
                     NDEREQTLNQMLVEMDGFETNLGVI"
     gene            complement(1087..1758)
                     /locus_tag="PK242_09530"
     CDS             complement(1087..1758)
                     /locus_tag="PK242_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOZ94299.1"
                     /translation="MKVKSKSKKVNKAWLADHINDPYVKLAQKEGYRARAAYKLKEID
                     ETLGLIRPGMRVVDLGSSPGAWSQYLRRRLAPAGAAVGALDGTIVALDILPMEPIEGV
                     HFLQGDFRDDEVLARLNEAVGGQPLDLVVSDMAPNLSGVESSDAARIAHLVELALDFA
                     LGHLKPGGALVVKLFHGSGYSQLVKLFKDSFKVVKPVKPKASRDRSAETFLVGIGPKP
                     PAETG"
     gene            1854..2327
                     /locus_tag="PK242_09535"
     CDS             1854..2327
                     /locus_tag="PK242_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOZ94300.1"
                     /translation="MPHIQLTPAERKAHRAEAHHLDPVVMVGADGLTPAVVKETDAAL
                     NAHGLIKLRVLGDDRAAREAMFQQLADQLNAAPIQHIGKLLVLWRPKPAKPRSLDEER
                     RPGPKDVKVIKHSKRGGQRPEVKVVRVLGNQRLTPGGKVKRAQPKQKSVKKGRAD"
     gene            2362..3117
                     /locus_tag="PK242_09540"
     CDS             2362..3117
                     /locus_tag="PK242_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ94301.1"
                     /translation="MSDNRQRHILCMKWGTKYGPEYVNRLYGMVRRHLAGEFQFICLT
                     DDASGVRPEVRCLPIPPLNLQLKPGQRDGAWKKLTTFEADLHGLTGTALFLDLDVVVV
                     GPLDDFFTQPGEFLIIHDYPRFWRFGERVVGNSSVYRFALGAHADVLAYFRTHMDEVQ
                     KQYRNEQDFLSHFLHRQGKLNYWPAAWCPSFKYHSIPTWPTNYWKAPIVPPGARIVIF
                     HGEVNPPDALAGKRNKRFAFIKPALWVADAWQG"
     gene            complement(3200..4093)
                     /locus_tag="PK242_09545"
     CDS             complement(3200..4093)
                     /locus_tag="PK242_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HOZ94302.1"
                     /translation="MDAPIVELTLNDWGQASSRPEWTAAVEAGKVLYFPQLRFQLEGH
                     ELALLREDMLAAGTRNVSLGADGVLKGAGGSADEQRALAAMIGRFRGQALQLVDGLLP
                     AYRGLLRVAPTSFRPRQVETRAQSIRADDQRLHVDAFPSRPNYGERILRVFANINPQG
                     VPRVWRVGEDFETVARQFLPRAKPYRLWQARALNALHVTKSLRSEYDHLMLQLHDLMK
                     RDERYQQSGGQVSMPFAAGSAWVCFSDQATHAVMSGQFMMEQTLHLPPGREANPQASP
                     LAILTRLVGRPLVGVGAGTPR"
     gene            complement(4098..4514)
                     /locus_tag="PK242_09550"
     CDS             complement(4098..4514)
                     /locus_tag="PK242_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HOZ94303.1"
                     /translation="MSDRLALLAAALWWGTLSAVIGFVVPLLFRLLPSKALAGQTAAV
                     LFTAQAWVSLVCGALLLLLLRGRAGAAGEVRARTTLGLVMAGMLLALLIEFAVAPRIV
                     ARVNLPLWHGLGTAMFALQWLCAGVVLWRLGGTPRR"
     gene            complement(4526..5002)
                     /gene="greA"
                     /locus_tag="PK242_09555"
     CDS             complement(4526..5002)
                     /gene="greA"
                     /locus_tag="PK242_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOZ94304.1"
                     /translation="MATFPITKRGAEMLKAELHRLKTKDRPEVINAIAEARAQGDLSE
                     NAEYEAAKDRQGFIEGRIKEVEGKLAAAQVIDPAGLDAGGRIVFGATVELEDEDSGEQ
                     VTYQIVGEDEADLKHGLINVGSPIARALIGKEEGDTAEVQAPGGVRHYEVIAVRYV"
     gene            5150..5857
                     /locus_tag="PK242_09560"
     CDS             5150..5857
                     /locus_tag="PK242_09560"
                     /inference="COORDINATES: protein motif:HMM:NF011765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HOZ94305.1"
                     /translation="MIQPTRRHLLHASAAMATTALAGRALAQGSCAVPTRDVQAAISP
                     DAALQRLREGNARFVAGQPIQCDLRAQVRASAGGQAPFAAVLGCIDSRVPPELVFDQR
                     LGDIFAARVAGNFVNDDILGSLEFATQLAGARLIVVLGHTECGAIKGAVDNAKLGHLT
                     GLLAQIRPSLERLHYRGTPSSKDQALVQRVAERNVQDAVTRLNAAPVLAALVQAGQLR
                     IVGAMHDVATGRIGWQV"
     gene            complement(5930..6904)
                     /locus_tag="PK242_09565"
     CDS             complement(5930..6904)
                     /locus_tag="PK242_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ94306.1"
                     /translation="MGIKSVLAFLALGLAAMASAQTANYPNRPVTLIVPAGAGGGTDV
                     VARVLADELGKRLGQAVVVDNKTGASGMLGTQAVARAAPDGHTLLVAYSTPIFYAHHI
                     FAKVPYDIRKDFEFISQLAATSLVMMVNADVPAKNMKEFMAWAQQNKGKVNYGSYGQG
                     SPGHLMSAYLSDSRKLDMTHVAYKSEAPHIQDLAGGVVSWGLGTIAAAQGALKDKRIR
                     PIAIYGPKRLAELPDVATMAEQGFADPEFNTVAWFTLLAPAGTPKPILQRLEKETVEI
                     IHSTAMKARFQVFGLESVPGGAARFHKDFDAVDPVIQKLVRISGVKAN"
     gene            complement(7086..>7811)
                     /gene="carB"
                     /locus_tag="PK242_09570"
     CDS             complement(7086..>7811)
                     /gene="carB"
                     /locus_tag="PK242_09570"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517569.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOZ94307.1"
                     /translation="VNPRASRTVPFVSKATGVQLAKVAARCMAGQTLDAQGIAAEVTP
                     PYFSVKEAVFPFVKFPGVDTILGPEMKSTGEVMGVGKTFGEAYIKAQLGAGERLPRPL
                     RADGTPAGKVFLTVKNSDKPQAVQIARELAGMGFGLVATRGTAQAIRDAGIACETVNK
                     VTEGRPHIVDMVKNDDISLVINTVEERRNAITDSRAIRTSALAARVPTITTIFGAEAA
                     VEGMKFMDRLGVISVQEMHAQLA"
BASE COUNT         1264 a         2732 c         2564 g         1251 t
ORIGIN      
        1 cgatcacgcc caggttggtc tcgaagccgt ccatctcgac cagcatctgg ttcagggtct
       61 gctcgcgctc gtcgttgcca ccgcccagtc cggcgccgcg ctggcggccg acggcgtcga
      121 tttcatcgat gaagatgatg cagggcgcgt tcttcttggc gttctcgaac atgtcgcgca
      181 cgcgcgccgc gcccacgccg acgaacattt cgacgaagtc cgagcccgag atgctgaaga
      241 acggcacctt ggcctcgccg gcgatggact tggccagcag cgtcttgccg gtgccgggcg
      301 ggccgaccag cagcaggccg cgcggaatgc ggccgcccag cttctggaac ttctgcgggt
      361 ccttgaggaa gtccaccact tccttgacct cttccttggc ctcgtcgcag ccggcgacat
      421 cggcaaaggt gatggtgttg ttgttttcgt ccagcatgcg cgccttgctc ttgccgaagc
      481 tgaaggcgcc gcccttgccg ccgccctgca tctggcgcat gaagtagatc cagacgccga
      541 tcagcagcag catcgggccc cacgacacca gcagtgtcat cagcagcgag ccttcttcgc
      601 gcggcttgac gtcgaacttg acgttgtggt tgatcagatc gcccaccagc ccgcgatcca
      661 ggtaggtggc ctgggtgcgc acgcggcggt cgtcgctcat cacggccacg atgtcggtgc
      721 cgccctgccc ttcctggatg gtggcctgct tgacgcggtt gttgcgcact tcttccagga
      781 agtcggaata ggccatgtgg ttggcgctgg ccatgcggcc gccgtcgaac tgtttgaaga
      841 cagtgaacag caccatggcg atcaccatcc agatggcgat cttcgagaac cattcgtttt
      901 tcaaggcctt gcgctccagt gggtcagtcc ggcgatgcca gacaggtgaa ggccatttta
      961 ggcttttcaa cccatggcgg gcgcccgtgc gccggggtaa ccgcagggac gaaatcccca
     1021 atttttcccg gcaccggggg cgcacagacg cctgccccgc cgccgcaggg ccgaaattga
     1081 tgcaaatcaa cccgtttcgg cgggcggctt gggtccgatg ccgaccagaa aggtctcggc
     1141 cgagcggtcg cgcgaggcct tgggcttgac cggcttgacc accttgaagc tgtccttgaa
     1201 cagcttgacg agttggctgt agccgctgcc gtgaaacagc ttcaccacca gcgcgccgcc
     1261 gggcttgagg tggcccaggg caaagtcgag cgccagttcc accaagtgcg cgatgcgcgc
     1321 ggcgtcgctc gactcgacgc ccgacaggtt gggcgccatg tccgacacca ccaggtccag
     1381 cggctgccca cccaccgctt cattcaggcg cgccagcacc tcgtcgtcgc ggaagtcgcc
     1441 ctgcagaaaa tgcacgccct cgatcggctc catgggcagg atgtcgagcg ccacgatggt
     1501 gccgtccagc gcgcccaccg ccgcgcccgc gggcgccagg cgccggcgca ggtactggct
     1561 ccaggcgccg gggctgctgc ccagatcgac cacccgcatg ccgggtcgga tcaggcccag
     1621 cgtctcgtcg atctccttga gcttgtaggc ggcgcgggcg cggtatccct ccttctgcgc
     1681 cagcttgacg taggggtcgt tgatgtggtc ggccagccag gccttgttga ccttcttgct
     1741 tttgctcttg actttcatgt aggcgtattc tggccgcacc ggcggcgtgg cagtccgccc
     1801 tggcgccgcg aactcggggc gcacgggcgc cggccgtgga cataatacgg cccatgccgc
     1861 acatccaact cacccccgcc gaacgcaagg cgcaccgcgc cgaagcgcac cacctggacc
     1921 cggtggtcat ggtcggcgcc gacggcctga cgcccgccgt ggtgaaggaa accgacgcgg
     1981 cgctcaacgc ccatggcctg atcaagctgc gcgtgctggg cgacgaccgc gccgcgcgcg
     2041 aggcgatgtt tcagcaactg gccgatcagc tcaacgccgc gcccatccag cacatcggca
     2101 agctgctggt gctgtggcgg cccaaaccgg ccaagccgcg aagcctggac gaagaacgcc
     2161 ggcccggccc caaggacgtc aaggtcatca aacacagcaa gcgcggtggc cagcggcccg
     2221 aagtcaaggt ggtgcgcgtg ctgggcaacc agcgcctgac gcccggcggc aaggtgaagc
     2281 gggcgcagcc gaagcagaag tcggtcaaga aggggcgcgc cgactgacgg cgcacgcttc
     2341 gattttcata gctgcctgcg catgtccgac aaccgccagc gccatattct gtgcatgaaa
     2401 tggggcacca agtatggccc cgaatacgtc aaccgcctgt acggcatggt gcgtcgccac
     2461 ctggcgggcg agttccagtt catctgcctg accgacgacg ccagcggtgt gcgccccgaa
     2521 gtgcgctgcc tgccgatccc gccgctgaac ctgcagctca agcctggcca gcgcgacggc
     2581 gcctggaaga agctcaccac cttcgaggcc gacctgcacg gcctgaccgg caccgccctg
     2641 tttctcgacc tggacgtggt ggtggtcggc ccgctggacg acttcttcac gcagccgggc
     2701 gagtttctga tcatccacga ctacccgcgc ttctggcgct tcggcgagcg cgtggtcggc
     2761 aactcgtcgg tctaccgctt cgcgctgggc gcgcacgccg acgtgctggc gtactttcgc
     2821 acccacatgg acgaggtgca aaagcaatac cgcaacgagc aggactttct gtcgcacttc
     2881 ctgcaccggc agggcaagct gaactactgg ccggcggcgt ggtgccccag cttcaagtac
     2941 cacagcatcc cgacctggcc gaccaactac tggaaggcgc ccatcgtgcc gccgggcgcg
     3001 cgcatcgtga tcttccacgg cgaagtcaac ccgccggacg cactggcggg caagcgcaac
     3061 aagcgctttg ccttcatcaa gccggccctg tgggtggccg acgcctggca gggctgaggc
     3121 gccgccggtt gatgggtgtt tcagggctcc agcgcccgtc cagattgcgc cagcagctct
     3181 ttaaacgata gcagtccggt cagcgcggcg tgcccgcgcc gacaccgacc agcggccgcc
     3241 cgaccagcct tgtgaggatc gccaacgggc tggcctgggg gttggcctcg cgccccggcg
     3301 gcaggtgcaa ggtctgctcc atcatgaact gcccgctcat caccgcgtgt gtggcctggt
     3361 ccgaaaagca cacccaggcg ctgccggcgg caaacggcat gctgacctgg ccaccgcttt
     3421 gctggtagcg ctcgtcccgc ttcatcaggt cgtgcagttg cagcatcagg tggtcgtact
     3481 cgctgcgcag cgacttggtg acgtgcaggg cgttgagggc ccgcgcctgc cacaggcgat
     3541 agggcttggc gcgcggcagg aactgccgcg ccacggtctc gaagtcctcg cccacgcgcc
     3601 acacgcgcgg cacgccctgc gggttgatgt tggcaaacac gcgcaggatg cgttcgccat
     3661 agttgggacg cgagggaaag gcatcgacgt gcaggcgctg gtcgtcggcg cgaatggact
     3721 gcgcgcgcgt ctccacctgg cgcggccgaa agctggtggg cgcgacacgc agcaggcccc
     3781 ggtacgccgg cagcaggcca tcgaccagct gcagcgcctg gccgcgaaag cgcccgatca
     3841 tggccgccag cgcccgctgt tcgtcggcgc tgccgcccgc gcccttgagc acgccatcgg
     3901 cgcccaggct gacgttgcgc gtgccggccg ccagcatgtc ttcgcgcagc agggccagct
     3961 cgtgcccttc gagctggaac ctcagctgcg gaaagtacag caccttgccc gcctcgacgg
     4021 cggccgtcca ctccggccgg ctgctcgcct ggccccagtc gttcagcgtc agctcgacga
     4081 tcggcgcgtc catcccctca cctccgtggt gtaccgccca ggcgccacag caccacgccg
     4141 gcgcacagcc actgcagggc gaacatggcc gtgcccagcc cgtgccacag cggcaggttg
     4201 acgcgcgcca cgatgcgcgg ggcgacggca aattcgatca gcagcgccag cagcatgccg
     4261 gccatcacca agcccagcgt ggtgcgcgcc cgcacctcgc ccgcagcgcc ggcgcggcca
     4321 cgcagcagca gcaacagcaa ggcgccacac accagcgaca cccaggcctg ggcggtgaac
     4381 agcaccgccg ccgtctggcc cgccagcgcc ttggacggca gcagcctgaa cagcaacggc
     4441 accacgaagc cgatcacggc cgacaaggtg ccccaccaca gcgcggcagc cagcagggcc
     4501 agacggtcgg acatggggcc gcccctcaga cgtagcgcac ggcgatcact tcgtagtgcc
     4561 gcacgccacc cggcgcctgc acttcggcgg tgtcgccctc ttccttgccg atcagcgcgc
     4621 gcgcgatcgg gctgccgacg ttgatcaggc cgtgcttcag gtcggcctcg tcctcgccga
     4681 cgatctggta ggtgacctgc tcgccgctgt cctcgtcctc cagctccacc gtggcaccga
     4741 agacgatgcg cccgccggcg tccagcccgg ccgggtcgat cacctgggcc gcggccagct
     4801 tgccctcgac ctccttgatg cgaccttcga tgaacccctg gcggtccttg gccgcctcgt
     4861 actcggcgtt ctcgctcaga tcgccctggg cgcgcgcctc ggcgatggca ttgatgacct
     4921 cgggacggtc cttggtcttc aggcggtgca gctcggcctt gagcatctcg gcgccgcgct
     4981 tggtgatcgg aaaggtggcc atgaaaaaat agaatgaaga aacgtgcgga tgagcagaaa
     5041 acacgaaacc atctgcgccg ggcacagatg gtctgaacag cgcacgattt taggccaacg
     5101 gcgtgccctg ccctgcgcca ccggccacct gcaacgaaac agccacgcca tgatccagcc
     5161 gacccgccgc cacctgctgc acgccagcgc cgccatggcc accaccgccc tcgccggccg
     5221 ggcgctggcg caaggcagct gcgccgtacc cacgcgcgac gtgcaggctg ccatcagccc
     5281 cgacgccgcg ctgcagcgcc tgcgcgaagg caacgcccgc ttcgtcgccg gccagcccat
     5341 ccagtgcgac ctgcgagcgc aggtgcgcgc cagcgccggc ggccaggcgc cgtttgccgc
     5401 cgtgctgggc tgcatcgaca gccgcgtgcc gcccgaattg gtgttcgacc agcgcctggg
     5461 cgacatcttt gccgcccgcg tggccggcaa cttcgtcaac gacgacatcc tgggcagcct
     5521 ggaatttgcc acccagctgg ccggcgcgcg cctgatcgtg gtgctgggcc acaccgagtg
     5581 cggcgccatc aagggcgcgg tggacaacgc aaaactcggc cacctgaccg gcctgctggc
     5641 ccagatccgc ccctcgctcg aacggctgca ctaccgcggc acgcccagct ccaaggacca
     5701 ggcgctggtg cagcgcgtgg ccgagcgcaa cgtgcaggac gccgtgacca ggctgaacgc
     5761 cgcgccggtg ctggcggcgc tggtgcaggc gggacagctg cgcatcgtgg gggcgatgca
     5821 cgatgtggcg acggggcgga tcggctggca ggtttgaagt tgggcgaggc ccgacgtttc
     5881 actccaacga cgcaagcaac ccaagccaac ccgccaccgg gtgccgagat cagttggcct
     5941 ttacgccaga gatcctgacc agcttctgga tcaccggatc aaccgcatcg aagtccttgt
     6001 ggaaccgcgc cgcgccacca ggcacggact ccaggccgaa gacctgaaaa cgcgccttca
     6061 tggccgtgga gtgaatgatt tccaccgttt ccttctccag gcgctgcaag atcggcttgg
     6121 gtgtgcccgc gggtgccagc agcgtgaacc aggcgacggt gttgaactcg gggtcggcaa
     6181 agccctgctc ggccatggtg gccacatcgg gcagttcggc caggcgcttg gggccataga
     6241 tggcgatggg gcgaatgcgc ttgtccttga gcgcaccctg ggccgcagca atggtgccca
     6301 ggccccagga gaccacgccg ccggccaggt cctgaatgtg cggcgcctcg ctcttgtagg
     6361 ccacgtgggt catgtccagc ttgcgcgagt cgctcaggta ggcgctcatc aaatggcctg
     6421 gcgagccctg gccataggag ccgtagttga ccttgccctt gttctgctgt gcccaggcca
     6481 tgaattcctt catgttcttc gccggcacat cggcgttgac catcatcaca aggctggtgg
     6541 ccgcaagctg ggagatgaac tcgaaatcct tgcggatgtc atacggcacc ttggcaaaaa
     6601 tgtggtgagc gtaaaagatg ggggtggaat aggccaccag cagcgtgtgg ccgtcgggcg
     6661 ccgcgcgcgc cacggcctgg gtgcccagca tgcccgaggc gcccgtcttg ttgtccacca
     6721 ccacggcctg gcccaggcgc ttgcccagct catcggccag cacgcgcgcc accacgtcgg
     6781 taccgccgcc ggcgccggcc ggcacgatca aggtgacggg ccgattcggg tagttggccg
     6841 tctgcgccga cgccatggcc gcgaggccca gggccaagaa ggccagtacg gatttgatgc
     6901 ccatgctttc ctcccaaaac aaagggaggg catgatgccc gccggtctgg ccgcgatcca
     6961 tcggaagata cccgcaggcc gcgcgccgcc agatggccat ggcagagggg cggatgcacc
     7021 ggtgggggtc gcgcgtcagg gtggatcggg tgtcactccg gcgtcactgc gaccccgcct
     7081 atcgctcagg ccagctgcgc atgcatctcc tgcaccgaaa tcacccccag ccggtccatg
     7141 aacttcatgc cttcgacggc cgcttcggcg ccgaagatgg tggtgatggt gggcacacgc
     7201 gcggccaggg cgctggtgcg gatggcgcgg ctgtcggtga tggcgttgcg gcgctcttcg
     7261 acggtgttga tgaccagcga gatgtcgtcg ttcttgacca tgtcgacgat gtgcgggcgg
     7321 ccttcggtga ccttgttgac ggtctcgcag gcgatgccgg cgtcgcggat ggcctgcgcc
     7381 gtgccgcgcg tggccaccag gccgaagccc atgccggcca gctcgcgcgc gatctgcacc
     7441 gcctgcggct tgtcgctgtt cttgaccgtc aggaacacct tgccggccgg cgtgccgtcg
     7501 gcgcgcagcg ggcgcggcag gcgctcgccg gcgcccagct gggccttgat gtaggcctcg
     7561 ccaaaggtct tgcccacgcc catcacctcg ccggtggact tcatctcggg gccgaggatg
     7621 gtgtccacgc cggggaactt gacgaacggg aacaccgctt ccttgacgct gaagtacggc
     7681 ggcgtgactt cggcggcgat gccctgcgcg tccagtgtct ggccggccat gcagcgcgcc
     7741 gccaccttgg ccagctgcac gccggtggcc ttgctgacga agggcacggt gcggctggcg
     7801 cgcgggttga c
//
LOCUS       DAOOOG010000125         7750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_11087_length_7750_cov_31.204288, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000125 DAOOOG010000000
VERSION     DAOOOG010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11087_length_7750_cov_31.204288"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..1452
                     /gene="paaA"
                     /locus_tag="PK242_09575"
     CDS             430..1452
                     /gene="paaA"
                     /locus_tag="PK242_09575"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HOZ94308.1"
                     /translation="MYTQSFSVPDDEGKAAGPKTVERPASEREAALQAQFDARIDADG
                     RIEPRDWMPEAYRKTLVRQISQHAHSEIVGMLPEGNWISRAPSLKRKAILIAKVQDEG
                     GHGLYLYSAAETLGTSREDMLEALHSGRAKYSSIFNYPTLNWADVGVIGWLVDGAAIM
                     NQIPLCRCSYGPYARAMVRVCKEESFHQRQGYEALLVMMKGTQAQRDMVQDAVNRWWW
                     KCLAMFGPPDADSPHSAQGMRWGIKRVSNDELRQKFIDATVPQAQVLGVTLPDPELKW
                     NEQRGHHDHGPIDWDEFWQTVGGHGPMNKERLATRVKAHDDGAWVREAALAHADKQQQ
                     RARKAA"
     gene            1469..1768
                     /gene="paaB"
                     /locus_tag="PK242_09580"
     CDS             1469..1768
                     /gene="paaB"
                     /locus_tag="PK242_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HOZ94309.1"
                     /translation="MSNAPALKDWPLWEVFVRSKQGLEHKHCGSLHASDAQHALQMAR
                     DVYTRRQEGVSIWVVPSHLIAASDPGEKDQLFEPASDKIYRHPTFYEIPEEVGHM"
     gene            1768..2610
                     /gene="paaC"
                     /locus_tag="PK242_09585"
     CDS             1768..2610
                     /gene="paaC"
                     /locus_tag="PK242_09585"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HOZ94310.1"
                     /translation="MDARVSTRAPIDYLLHLADNALVLGQRNAEWCGHAPILEEDMAM
                     ANNSLDLIGQARLLYQYAAERLAGDVRAPRGAHWPAGPLTEDTLAYFRDVPEFRNYTL
                     LELPHSPPLVPTGVGDRDWGVTIVRNFLYSALMVLVWERLQRSADPQLAAIAAKSLKE
                     VRYHLRHARDWLVRLGDGTDASQRRAQAALDWLLPFTDEFWTASTHETEARDNASGVD
                     MAALRADWQALVNDALAEATLTRPAAQPGYVSQGKQGLHSEHLGYLLAEMQSLARAHP
                     GATW"
     gene            2628..3227
                     /gene="paaJ"
                     /locus_tag="PK242_09590"
     CDS             2628..3227
                     /gene="paaJ"
                     /locus_tag="PK242_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HOZ94311.1"
                     /translation="MSPIDLQRPTGEREQLSNQEQARLWALLDTLTDPEIPVVTLREM
                     GILRALRRGSDGAVEVVITPTYSGCPAMDQIADDIAATLRDAGVPGRVVTQLAPAWTT
                     DWMSEAARDKLRAYGIAPPRCASRRGGESVIHFAPKSRAAGARLAGADGPSDAEDAVA
                     CPQCGSTHTTETSHFGSTACKALYRCLDCLEPFDHFKSI"
     gene            3238..4341
                     /gene="paaK"
                     /locus_tag="PK242_09595"
     CDS             3238..4341
                     /gene="paaK"
                     /locus_tag="PK242_09595"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HOZ94312.1"
                     /translation="MSAPRFHDLTVARINPEAAGAVAITFAVPPDLHGQFDFQPGQFL
                     TLRATIDGQDVRRNYSICSTRSRYARARELEVGIRPMEGGLFSNWAATQLRPGDTLAV
                     MPPDGRFTIRKPRALHRVGFAAGSGITPILSIIASTLEDSDSAKFTLVYGNRRMGSVM
                     FNEALQDLKDKYKDRLTLIHVLSRQAQEVPLLEGRIDAAKVQQLLDTLLPAKSMDEVF
                     ICGPEAMLEATESALRAAGVPAERIYTERFSSPRLDELSPEARRAAIAQPDPEAPAGQ
                     VALTVLIDGKPHELAMRRDQHVLDVALSAGLDAPYSCKAGVCCTCRAKVLEGSVSMDK
                     NFTLEEEEMAQGFVLSCQARPTSDRLVVSYDER"
     gene            complement(4397..5074)
                     /locus_tag="PK242_09600"
     CDS             complement(4397..5074)
                     /locus_tag="PK242_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOZ94313.1"
                     /translation="MPRDTSPGARLRGTPVSGSAHRPPARRLLCLGALWLAAGPLAAQ
                     TVSLNTSTPAVAEPCSSAKAVHDMRCPNYTGFKPRVGGLAGARAPAGRAADDVAEGLA
                     LPERPPLGLRGGRDLQRGMASWYGPGFHGRLTASGERFDQNDLTAAHKTLPFGTRVLV
                     HSPRTGKQVVVRINDRGPFVRGRVIDLSKAAARMLGLHALGHDAVVLRELTPDAGDQA
                     AAADRAG"
     gene            complement(5182..6024)
                     /gene="metF"
                     /locus_tag="PK242_09605"
     CDS             complement(5182..6024)
                     /gene="metF"
                     /locus_tag="PK242_09605"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOZ94314.1"
                     /translation="MSVNLSLEFFPPKTAEGADKLRAVRARLYALQPEFCSVTFGAGG
                     STQEGTLDTVRAIVAEGWPGVPHLSCIGQTAASIRERLASYRAAGVRRLVALRGDLPS
                     GYGMGGEFRHASDLVAFIRAETGDHFRIAVAAYPEFHPQARSPEADLDAFAAKVGAGA
                     DLAITQYFFNADAYFRFVDEARRRGVTVPIVPGIMPITNSTQLLRFSDSCGAEVPRWI
                     RLRLQGFGDDSAAIQDFGADVVAALCRRLHEGGAPGFHFYTLNQSAGVLELCRRGGWG
                     APFI"
     gene            complement(6021..6827)
                     /locus_tag="PK242_09610"
     CDS             complement(6021..6827)
                     /locus_tag="PK242_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOZ94315.1"
                     /translation="MRADQRLVQAGLATSRAQAQRLIDAGARWRSAADAPWQPVRKSG
                     QELPPDAELQLLDAREAAYVSRGGLKLEGALRATGLRVAGWRCLDVGQSTGGFTDCLL
                     AHGAAQVTGVDVGHGQLHPRLRSDARVRVLEGVNARHLDASVLIAAGADQTGAEGQAG
                     THFDLIVGDLSFISLTLVLPALLPLLGSGGQLLLLVKPQFELQPADIGKGGIVRDPAL
                     YAQVEQRLRQACAALGLAVHHWLPSPITGGDGNREFFLHAARAATTCDTP"
     gene            complement(6827..7120)
                     /locus_tag="PK242_09615"
     CDS             complement(6827..7120)
                     /locus_tag="PK242_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94316.1"
                     /translation="MTRWLHAWGWVVLWLWIVGRSGRAFPIGLGSALVLAVLLVPGIC
                     LPLVLMRREDAPDLSGELSAPLAGLAVGIVGLHVLAAGSIALAEWRLWPQGDD"
     gene            complement(7136..>7750)
                     /locus_tag="PK242_09620"
     CDS             complement(7136..>7750)
                     /locus_tag="PK242_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOZ94317.1"
                     /translation="AQALRALSAQVWVTEIDPICALQAAMEGFRVVTMDEAAPLADIF
                     VTATGNKDVITYAHMAAMKDQAIVCNIGHFDNEIDVASLEARCQWEEIKPQVDHVIFE
                     GGKRIILLAKGRLVNLGCATGHPSYVMSSSFANQTIAQIELFTRPDAYAAGRVYVLPK
                     HLDEKVARLQLAKLNARLTELSDEQARYIGVAKAGPYKPDSYRY"
BASE COUNT         1270 a         2796 c         2653 g         1031 t
ORIGIN      
        1 acttctggtg gtacttcaag caggacatgg tgccctacac caaacccctg tggaataccc
       61 tcaatcaggc catcagcgcc aacccttgag gggcgccaac gattccgcag tgccggcaag
      121 aaccgcccca acccaccacc ggccctgccg gcccatggtg gccgcagccc ctgcggcggc
      181 ggtgtctggg gcccggatcg gccgctttgg gccgctctcg tcggcactcg gcatgcatgc
      241 ccatctgcgc agtcccggcc cgcggggcgc gcagcggcgg acgcccgggt aaaccctggg
      301 cgatcccgcc tcaattaacc aaccgttcgg tcggttaact cactatactt tgggtcttca
      361 cgcccgcgtg gtccggtcat tcgaggctgc gcgggcttgg tgctgtcggt cacccaggag
      421 atccgcgacg tgtacaccca atccttttcc gttcccgacg acgagggcaa ggccgccggg
      481 ccgaagacgg tcgagcgacc ggccagcgag cgcgaggcgg cgctgcaggc gcagttcgac
      541 gcccgcatcg acgccgacgg ccgcatcgag ccgcgcgact ggatgcccga ggcctaccgc
      601 aagacgctgg tgcgccagat cagccagcac gcgcattccg agatcgtcgg catgctgccc
      661 gagggcaact ggatcagccg cgcgcccagc ctcaagcgca aggccatcct gatcgccaag
      721 gtgcaggacg aaggcggcca tggcctgtac ctgtacagcg ccgctgagac gctgggcacc
      781 tcgcgcgagg acatgctgga ggcgctgcac tcgggccgcg ccaagtacag cagcatcttc
      841 aactacccga cgctgaactg ggccgacgtc ggcgtgatcg gctggctggt cgatggcgcg
      901 gcgatcatga accagatccc gctgtgccgc tgctcctacg gcccctacgc gcgcgccatg
      961 gtgcgcgtgt gcaaggaaga gagcttccac cagcgccagg gctacgaggc cctgctggtc
     1021 atgatgaagg gcacccaggc gcagcgcgac atggtgcagg acgccgtcaa ccgctggtgg
     1081 tggaaatgcc tggccatgtt cggcccgccc gacgccgaca gcccgcacag cgcgcagggc
     1141 atgcgctggg gcatcaagcg cgtcagcaac gacgagctgc gccagaagtt catcgacgcc
     1201 accgtgccgc aggcgcaggt gctgggcgtg acgctgcccg accccgagct gaagtggaac
     1261 gaacagcgcg gccaccatga ccacggcccg atcgactggg acgagttctg gcagaccgtg
     1321 ggcggccacg gccccatgaa caaggagcgc ctggccacgc gcgtgaaggc gcacgacgac
     1381 ggcgcctggg tgcgcgaggc cgccctggcc cacgccgaca agcagcagca acgcgcccgc
     1441 aaggccgcct gatccccgag gagaaaccat gagcaacgca cccgcactga aggactggcc
     1501 cctgtgggag gtcttcgtcc gcagcaagca aggcctggag cacaagcact gcggcagcct
     1561 gcacgccagc gacgcgcagc acgcgctgca gatggcgcgc gacgtctaca cgcgcaggca
     1621 ggaaggcgtg tcgatctggg tcgtgccctc gcacctgatc gccgccagcg acccgggcga
     1681 aaaagaccag ctgttcgagc cggccagcga caagatctac cgccacccga ccttctacga
     1741 aatccccgaa gaagtggggc acatgtgatg gacgcccgcg tcagcacccg cgccccgatc
     1801 gactacctgc tgcacctggc cgacaacgcc ctggtgctgg gccagcgcaa cgccgaatgg
     1861 tgcggccacg cgcccatcct ggaagaggac atggcgatgg ccaacaacag cctggacctg
     1921 atcggccagg cgcgcctgct ctaccagtac gcggccgagc gactggccgg cgacgtgcgg
     1981 gcgccgcgcg gcgcccactg gccggccggt cccctgaccg aagacacgct ggcctacttc
     2041 cgcgacgtgc ccgaattccg caactacacc ctgctggagc tgccacacag cccgccgctg
     2101 gtgcccaccg gcgtgggcga ccgcgactgg ggcgtcacca tcgtgcgcaa cttcctctac
     2161 agcgcgctga tggtgctggt gtgggagcgc ctgcagcgct cggccgaccc ccagctggcg
     2221 gccatcgccg ccaagagcct gaaggaagtg cgctaccacc tgcgccacgc gcgcgactgg
     2281 ctggtgcgcc tgggcgacgg caccgacgcc tcgcagcgcc gcgcgcaggc cgcgctggac
     2341 tggctgctgc ccttcaccga cgagttctgg accgccagca cgcacgaaac cgaagcgcgc
     2401 gacaacgcca gcggcgtcga catggcggcg ctgcgcgccg actggcaggc gctggtcaac
     2461 gacgcgctgg ccgaggccac gctgacccgc ccggccgcgc agccgggcta cgtcagccag
     2521 ggcaagcaag gcctgcattc ggagcacctg ggctacctgc tggctgaaat gcagagcctg
     2581 gcgcgcgccc acccgggcgc cacctggtaa gcgacgccgg ccgccccatg agcccgatcg
     2641 acctccagcg cccaactggc gagcgcgagc agctatccaa tcaggagcaa gccagactct
     2701 gggcgctgct cgacaccctg accgacccgg agatcccggt ggtcacgctg cgcgagatgg
     2761 gcatcctgcg cgcgctgcgg cggggcagcg acggcgcggt ggaggtggtc atcaccccca
     2821 cctacagcgg ctgcccggcc atggaccaga tcgccgacga catcgccgcc accttgcgcg
     2881 acgccggcgt gcccggccgt gtggtgaccc agctggcgcc ggcctggacc accgactgga
     2941 tgagcgaggc cgcgcgcgac aagctgcgcg cctacggcat cgcgccaccg cgctgcgcca
     3001 gccgccgcgg cggcgaatcg gtgatccatt ttgctcctaa atcaagagct gccggcgctc
     3061 gtctggcggg cgccgacggc ccatcggatg ccgaagatgc ggtggcctgc ccgcaatgcg
     3121 gctccaccca caccaccgaa acctcgcatt tcggctccac cgcctgcaag gcgctgtacc
     3181 gctgcctcga ctgcctggag ccgttcgacc atttcaagag catctgagga gacccgcgtg
     3241 tccgctcccc gctttcacga cctcaccgtc gcccgcatca accccgaagc cgctggcgcc
     3301 gtggccatca ccttcgccgt gccgcccgac ctgcacgggc agttcgactt ccagcccggc
     3361 cagtttctga ccctgcgcgc caccatcgac ggccaggacg tgcggcgcaa ctactcgatc
     3421 tgctccacgc gcagccgcta cgcgcgcgcg cgcgagctgg aagtgggcat ccgaccgatg
     3481 gaaggcggcc tgttctccaa ctgggccgcc acccagctgc gcccgggcga caccctggcc
     3541 gtgatgccgc ccgatggccg cttcaccatc cgcaagccgc gcgccctgca ccgcgtgggt
     3601 tttgccgccg gctcgggcat cacacccatc ctgtcgatca tcgccagcac actggaagac
     3661 agcgacagcg ccaagttcac cctggtctac ggcaaccgcc gcatgggcag cgtgatgttc
     3721 aacgaagcgc tgcaggacct gaaggacaag tacaaggatc gcctcaccct gatccacgtg
     3781 ctgtcgcgcc aggcgcagga ggtgccgctg ctggaaggcc gcatcgacgc cgccaaggtg
     3841 cagcagctgc tcgacaccct gctgccggcc aagagcatgg acgaggtctt catctgcggc
     3901 cccgaagcca tgctggaggc caccgaaagc gcgctgcgcg ccgccggcgt gccggccgag
     3961 cgcatctaca ccgagcgctt cagctccccg cgcctggacg agctcagccc cgaggcgcgc
     4021 cgcgccgcca tcgcccagcc cgaccccgag gcgccggccg gccaggtggc gctgacggtg
     4081 ctgatcgacg gcaagccgca cgagctggcc atgcggcgcg accagcatgt gctggacgtg
     4141 gccctcagcg ccgggctgga cgcgccctat tcctgcaagg ccggcgtgtg ctgcacctgt
     4201 cgcgccaagg tgctggaagg cagcgtcagc atggacaaga acttcaccct ggaggaagag
     4261 gaaatggccc agggcttcgt gctgagctgc caggcccgcc ccaccagcga ccggctggtg
     4321 gtcagctacg acgagcgctg agcccgccgc aatctgccac gggcgctggt gccggttgac
     4381 ctgatggcga ccgggatcag ccggcgcggt cggcggcggc agcctgatcg cccgcatcgg
     4441 gtgtcagctc gcgcagcacc acggcgtcgt gacccagggc gtgcaggccc agcatgcggg
     4501 cggcggcctt gctcaggtcg atcacccggc ccctgacgaa cggcccgcgg tcgttgatgc
     4561 gcaccaccac ctgcttgccg gtgcgcgggc tgtgcaccag cacgcgggtg ccgaaaggca
     4621 gggtcttgtg ggcggcggtg agatcgttct ggtcgaagcg ctcgccgctg gcggtcaggc
     4681 gcccgtgaaa accggggccg taccacgagg ccatgccgcg ttgcaggtcg cgcccgcccc
     4741 gcagaccgag cggcgggcgc tcgggcaggg ccaggccttc ggccacgtca tcggcggcgc
     4801 ggccggccgg cgcacgcgcg cccgccagcc cgcccacgcg cggcttgaag ccggtgtagt
     4861 tggggcagcg catgtcgtgc accgccttgg ctgaagagca aggctcggca acggcagggg
     4921 tggaagtgtt caggctcacc gtctgggccg ccagcggccc ggctgccagc cacagggcgc
     4981 cgagacacag cagtcggcgg gctggcggac ggtgggcact tcccgaaacc ggcgtgccgc
     5041 gcaagcgggc gccgggagag gtgtcccgtg gcatggggcc gattttaacg gcgccaccgc
     5101 cccagccagg cgctccggac cccccaatcc agggtttcag acggtaaaac ccagataaaa
     5161 ccgttggcat taaaccaatg gttatatgaa aggggcgccc cagcctcctc ggcggcacag
     5221 ttccagcacc ccggccgact ggttgagggt gtagaagtga aagcccggcg cgccgccctc
     5281 gtgcaggcgg cggcacagtg cggccaccac gtcggcgccg aagtcctgga tggcggcgct
     5341 gtcgtcgccg aaaccctgca gccgcaggcg gatccagcgc ggcacctcgg cgccgcagct
     5401 gtcggaaaag cgcagcagct gggtgctgtt ggtgatcggc atgatgccgg gcacgatcgg
     5461 caccgtgacg ccgcgccggc gcgcctcgtc gacgaagcga aagtaggcgt cggcgttgaa
     5521 gaagtattgc gtgatggcca ggtccgcccc ggcgcccacc ttggcggcga aggcgtccag
     5581 gtcggcctcg ggcgagcgcg cctgcgggtg gaactcaggg taggcggcca ccgcgatgcg
     5641 gaagtgatcg ccggtttcgg cacggatgaa ggccaccagg tcgctggcgt ggcggaattc
     5701 gccgcccatg ccgtagccgc tgggcagatc gccgcgcagg gccaccagtc gccgcacccc
     5761 ggcggcgcgg tagctggcca ggcgctcgcg gatgctggcg gcggtctggc cgatgcacga
     5821 caggtggggc acgccgggcc agccttcggc gacgatggcg cgcaccgtgt ccagcgtgcc
     5881 ttcctgcgtc gagccgccgg cgccgaaggt gacggagcag aactcgggtt gcagcgcgta
     5941 cagccgggcg cgcacggcac gcagcttgtc ggcgccctcg gccgtcttgg gggggaagaa
     6001 ttcgagactg aggttgacgc tcatggggta tcgcaagtcg tcgccgcgcg cgcggcatgg
     6061 agaaagaatt cccggttgcc gtcgccaccg gtgatggggc tgggcaacca gtggtgcacg
     6121 gccaggccga gcgcggcgca ggcctggcgc agccgctgct cgacctgcgc gtacagggcc
     6181 ggatcgcgca cgatgccgcc cttgccgatg tcggccggct gcagctcgaa ctgcggcttg
     6241 accagcagca gcaactgccc gccgctgccc agcagcggca gcagcgccgg cagcaccagg
     6301 gtcagcgaaa tgaaggacag atcgcccacg atcaggtcga aatgagtgcc agcttggcct
     6361 tcagcgcccg tctggtcagc gccagcagct atcaaaacag aagcatccag gtgccgggcg
     6421 ttgacacctt cgagcacccg cacgcgggcg tcgctgcgca ggcgcggatg cagctggccg
     6481 tggcccacgt cgaccccggt cacctgcgcc gcaccgtgcg ccagcaggca gtcggtgaag
     6541 ccgccggtgg actgcccgac gtccaggcag cgccagccgg ccacgcgcag cccggtggcg
     6601 cgcagcgcgc cctccagctt gaggccaccg cgcgagacgt acgccgcctc gcgcgcatcc
     6661 agcagttgca gctcggcgtc gggcggcagc tcctggccgc tcttgcgcac cggctgccag
     6721 ggcgcgtcgg cggcgctgcg ccagcgcgca ccggcgtcga tcaggcgctg cgcctgagcg
     6781 cgcgaggtgg ccagcccggc ctgcaccagg cgctggtcgg cgcgcatcag tcgtcgccct
     6841 gcggccacag ccgccattca gccagcgcga tgctgcccgc ggccagcacg tgcaggccga
     6901 caatgcccac cgccagaccc gccagcggcg ccgacagctc gcccgacagg tccggcgcgt
     6961 cctcgcgccg catcagcacc agcggcagac agatgcccgg caccagcagc accgccagaa
     7021 ccagggcgct gcccaggccg atcggaaagg cgcgaccgct gcgtccgacg atccacagcc
     7081 acagcaccac ccagccccag gcatgcagcc agcgcgtcat ggggtccgcc cgggatcagt
     7141 agcggtagct gtcgggcttg tacggcccgg ccttggccac gccgatgtag cgcgcctgct
     7201 cgtcgctcag ctcggtcagg cgcgcgttca gcttggccag ctgcaggcgc gcgacttttt
     7261 cgtccaagtg cttgggcagc acgtagacgc ggccagcggc gtaggcgtcg gggcgggtga
     7321 acagctcgat ctgggcgatg gtctggttgg cgaagctgga gctcatcacg tagctggggt
     7381 ggccggtggc gcagcccagg ttcaccaggc gccccttggc cagcaggatg atgcgcttgc
     7441 cgccttcgaa gatgacgtgg tcgacctgcg gcttgatctc ctcccactgg cagcgcgctt
     7501 ccaggctggc gacgtcgatc tcgttgtcga agtggccgat gttgcagacg atggcctggt
     7561 ccttcatggc cgccatgtgg gcgtaggtga tgacgtcctt gttgccggtg gcggtgacga
     7621 agatgtcggc cagcggcgcc gcctcgtcca tggtgaccac gcgaaagcct tccatggccg
     7681 cctgcagcgc gcagatgggg tcgatctcgg tcacccacac ctgggccgac agcgcgcgca
     7741 gcgcctgcgc
//
LOCUS       DAOOOG010000126         7328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_12016_length_7328_cov_31.275815, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000126 DAOOOG010000000
VERSION     DAOOOG010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12016_length_7328_cov_31.275815"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="PK242_09625"
     CDS             <1..1331
                     /locus_tag="PK242_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOZ94318.1"
                     /translation="AWGIVVAGVGGTGVITIGQLLGMAAHLEGKGIVTQDAAGLAQKG
                     GATWSHVLIGATQDDIRTTRVSAAAADLIIGCDPIVTANKETVARMREGRTHVALNAH
                     ATPTAAFVKDGGWQNPQARCAADIARAVGEQGLASFDADRLAGRLLGDTIYVNPMILG
                     YAWQRGWVPLRRESLLRAIELNNVQVDNNKAAFEWGRQAAHDGARVEQALAGAGGEQV
                     VAFKPRETLESIVARRVEFLTGYQNAAYAERYRAFVARVQQQEAALGRTQLTEAVAQG
                     LFKLMAYKDEYEVARLHSDPAFLARIDAMFEGDYKLHYHLAPPMTAQRNERGELVKKK
                     YGSGMGRVFRLLARLKGLRGGALDLFGKTEERQTERALIEQYRASIDELLATLNADNH
                     AIAVEIARIPQAIKGFGHVKERHLKAARARWDELRAQYRQPPVAPARQIA"
     gene            1556..1885
                     /gene="yajC"
                     /locus_tag="PK242_09630"
     CDS             1556..1885
                     /gene="yajC"
                     /locus_tag="PK242_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOZ94319.1"
                     /translation="MFISNAYAQAAPAAPGADLASTFTSMLPLVLMFVVLYFIMIRPQ
                     MKKQKEHRAMIDALAKGDEVVTAGGLMGRVTALSEGQIGLQIASGVEVQMQRSAIVQV
                     LPKGTLK"
     gene            1950..3809
                     /gene="secD"
                     /locus_tag="PK242_09635"
     CDS             1950..3809
                     /gene="secD"
                     /locus_tag="PK242_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOZ94320.1"
                     /translation="MNRYPLWKYIVILITLVVAALYTLPNFFGEAPAVQVSAGKATTR
                     IDGGTLSRVEGALQAAGLTPQRLTLEGSSVWARFDTPDTQLKAKDVIERALNPDAADP
                     AHIIALNLVSRSPAWLAAINAQPMYLGLDLRGGVHFMLQVDMRTAITKRLDTLANDIR
                     VALREKDVRHGGISREGNQIEIRARDADTLEAARRVIVDTQPDLQVEPTQGTEPRLVA
                     SFKPQALQRVQADAVKQNITTLHNRVNELGVAEPVIAQQGVDRVVVQLPGVQDTAKAK
                     DILGRTATLEMRLVDESAEGRAAETGGPVPFGSEKFLERDGRPVIVKRQVILTGDSLT
                     DAQPGFDQQTQQPKVDLTVDAKGGRVMRDVSRENLKKRMAIVLFEKGKGEVLTAPVIQ
                     AELGTRFQISGSMTVSEANDLALLLRAGSLAAPMEIIEERTIGPSLGADNISKGFHSV
                     MWGFLAIVVFMIAYYMLFGVFSAVALSVNLLLLVAILSMLQATLTLPGMAAVALTLGM
                     AIDANVLINERVREELRGGASPQAAIHAGYDRAWATILDSNVTSLIAGLALLAFGTGP
                     VRGFAVVHCIGILTSMYSAVFFSRGLVNLWYGRQKKLKSVSIGTVWRPAPEKV"
     gene            3850..4800
                     /gene="secF"
                     /locus_tag="PK242_09640"
     CDS             3850..4800
                     /gene="secF"
                     /locus_tag="PK242_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOZ94321.1"
                     /translation="MEFFRIGRDIPFMRYARLFNAISIVTFLLAVVFLFTRGLHLSVE
                     FTGGTVMEVAYSQPADLAQVRGTVAALGYGDVQVQNFGTARDVIIRLPVQQGKSSAQQ
                     SEQVLAALRSASPDVQLRRTEFVGPQVGDELARDGLYALAFVVVGIMIYLSMRFEWKY
                     AVAGIIANLHDVVIILGFFALFQWEFSLSVLAAILAVLGYSVNESVVIFDRIRENFRR
                     YRKMSTVEIINNSITANISRTIITHGSTQIMVLSMLLFGGETLHYFAVALTIGILFGI
                     YSSVFVAAAIAMWLGIRREDLVKTGRKDGDPGDPNAGAVV"
     gene            4859..7300
                     /locus_tag="PK242_09645"
     CDS             4859..7300
                     /locus_tag="PK242_09645"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOZ94322.1"
                     /translation="MVSTPPPPSPHRLAQQVRERFLQVLDAAVIELGERIQTHLTGQA
                     QGGIRGATLADMQRAMEAEAAFRRARTAWVTAARRHWRDALTQMTDSRAARRSLPGDL
                     GLVDDDSVERKIAASRLGAAVQDAAGAEFNDLRLRMQALERSTDLPARDVLRPDTFAQ
                     QLLKAWRDAGLDRPMWVLVHPEIQAAMIERLVPLYGQANAFLIERGVLAEIDLKALVR
                     RGDGDAGPVAGARASAVAGARDRAAPGPAENAASRESGARSGGAGLFGDGRSARGDAF
                     TTHGRANAADDTQTPDVRHSALARAAQETRMMTAISPMTRVRQRAHGVLGQLRRLLTD
                     QLANFDLAAPAEPPSPPLARAMAEAAPFAVTELLDRSDADVTIVQPVASVEAVATRLR
                     QRATELKAKAERPSEKATIEIVALMFQAILAEERIAAAIRVWFARLQIPVLRLALAEP
                     DFFASIQHPARQLIDRMGACALGFDAAQVGGSRLEREIKRIVQVIEQYPETGRRVYQL
                     VLEEFKKFLGRSLTEGGSAQQMATLAQQIEQKEALTVQYTIELRRMLEPVPVPEPVRE
                     FLFRVWCEVLAMAAVRRGPQAPDTLRFKQAAADLLWAVSPKADRSERGRVVQQLPTLL
                     HALREGMEGLALSTSDQDAHIKVLNDAVVQAFVSRGEGLSAERLAELSRNLAGLEDVV
                     SDDAQGDVLLDPGVIELMFGAEGDALEVIASGGSQPGEGMLHWAGELELGAWFTLDYL
                     GSVTQVQYVWRSKRGQLHLFGDGSGKSYLVQTWRLASYLQAGLLVPVEDETLTVRATR
                     EALARLNAEPDRLLN"
BASE COUNT         1175 a         2486 c         2569 g         1098 t
ORIGIN      
        1 gcgcctgggg catcgtggtg gccggcgtgg gcggcaccgg cgtcatcacc atcggtcagt
       61 tgctgggcat ggcggcgcac ctggaaggca agggcatcgt cacccaggac gccgccggcc
      121 tggcgcaaaa gggcggcgcc acctggagcc atgtgctgat cggcgccacg caggacgata
      181 tccgcaccac gcgggtgagc gcggcggcgg ccgacctcat catcggctgc gacccgatcg
      241 tcaccgccaa caaggaaacc gtggcccgca tgcgcgaggg gcgcacgcac gtggcgctga
      301 acgcccacgc cacgcccacg gcggccttcg tcaaggacgg cggctggcag aacccgcagg
      361 cgcgctgcgc cgccgacatc gcccgcgccg tgggcgaaca gggcctggcc agcttcgacg
      421 ccgaccgcct ggccggccgg ctgctgggcg acaccatcta cgtcaacccc atgatcctgg
      481 gctacgcctg gcagcgcggc tgggtgccgc tcaggcgtga gtcgctgctg cgcgccatcg
      541 aactgaacaa cgtgcaggtc gacaacaaca aggcggcctt cgaatggggc cgccaggcgg
      601 cgcacgacgg cgcgcgcgtc gagcaggcgc tggccggcgc cggcggcgag caggtggtgg
      661 cgttcaagcc gcgcgagacg ctggaatcca tcgtcgcccg gcgcgtcgaa ttcctcaccg
      721 gctaccagaa cgccgcctat gccgagcgct accgcgcctt cgtggcccgg gtgcagcaac
      781 aggaggcggc gctgggcaga acccagttga ccgaggcggt ggcgcagggc ctgttcaagc
      841 tgatggccta caaggacgag tacgaggtgg cgcgcctgca cagcgacccg gccttcctgg
      901 cgcgcatcga cgccatgttc gaaggcgact acaagctgca ctaccacctg gcgccgccca
      961 tgacggccca gcgcaacgag cgcggcgagc tggtcaagaa gaaatacggc tccggcatgg
     1021 ggcgggtgtt tcggctgctg gcgcgcctca agggcctgcg cggcggcgcg ctggatctgt
     1081 tcggcaagac cgaggagcgc cagaccgagc gcgcgctgat cgagcagtac cgcgccagca
     1141 tcgacgagct gctggccacg ctgaacgccg acaaccacgc catcgcggtc gagatcgcgc
     1201 gcattccgca ggccatcaag ggcttcggcc acgtcaagga gcgccacctg aaggcggcgc
     1261 gtgcgcgctg ggacgagctg cgggcgcagt accgccagcc gcccgtcgcg ccggcgcggc
     1321 agatcgcctg aaattcgggc gaaatatggg tttcgcgacc gcccagtctg cgccggcagc
     1381 tatgatattg atagtaatcg atccatgatt cgatggctgc gcgttgggcc cccagcgcgg
     1441 ccccgcgcgc cagcgcgcct tacaatgcac ggttgcctgc gcgcgccccg ccgggcgcgc
     1501 agctgcgcgc cacgcgcgcg atccatccat caccgcccat ttcaggagcc tgccggtgtt
     1561 catctccaac gcctacgccc aagccgcccc cgccgccccc ggggccgacc tcgcctccac
     1621 cttcaccagc atgctgccgc tggtgctgat gttcgtggtg ctgtacttca tcatgatccg
     1681 cccgcagatg aagaagcaga aggaacaccg cgccatgatc gacgcgctgg ccaagggcga
     1741 cgaggtggtg accgccggcg gcctgatggg gcgcgtcacg gcgctgtcgg aaggccagat
     1801 cggcctgcag atcgccagcg gcgtcgaggt gcagatgcag cgcagcgcca tcgtgcaggt
     1861 gctgcccaag ggaaccctga agtaagcccg gacgccggcg ccggccctcg cgccggcgcg
     1921 catctggccg cccctatcct gccgtcgcca tgaaccgcta ccccctctgg aaatacatcg
     1981 tcatcctgat caccctggtg gtggcggcgc tctacaccct gcccaacttc ttcggcgagg
     2041 cgccggcggt gcaggtgtcg gccggcaagg ccaccacccg gatcgatggc ggcacgctgt
     2101 cgcgtgtgga aggcgcgctg caggcggccg gcctgacgcc ccagcgcctg acgctggaag
     2161 gcagctcggt gtgggcgcgc ttcgacacgc ccgacaccca gctcaaggcc aaggacgtca
     2221 tcgagcgcgc actcaacccc gacgcggccg acccggccca catcattgcg ctcaacctgg
     2281 tgtcgcgttc gccggcctgg ctggcggcca tcaatgccca gcccatgtac ctggggctgg
     2341 atctgcgcgg tggcgtgcac ttcatgctgc aggtggacat gcgcacggcc atcaccaagc
     2401 gcctggacac gctggccaac gacatccggg tggcgctgcg cgagaaggac gtgcgccacg
     2461 gcggcatcag ccgcgaaggc aaccagatcg agatccgcgc gcgcgatgcc gacacgctgg
     2521 aggcggcgcg ccgcgtcatc gtcgacaccc agcccgacct gcaggtggag ccgacccagg
     2581 gcaccgagcc gcggctggtg gccagcttca agccgcaggc gctgcagcgc gtgcaggccg
     2641 atgcggtcaa gcagaacatc accacgctgc acaaccgcgt caacgagctg ggcgtggccg
     2701 aaccggtgat cgcccagcaa ggggtggacc gcgtggtggt gcagctgccc ggcgtgcagg
     2761 acacggccaa ggccaaggac atcctggggc gcaccgccac gctggagatg cggctggtcg
     2821 acgaaagcgc cgaaggccgt gccgccgaaa ccggcggccc ggtgccgttc ggctccgaaa
     2881 aattcctcga gcgcgacggc cgcccggtga tcgtcaagcg ccaggtgatc ctgaccggcg
     2941 acagcctgac cgacgcccag cccggcttcg atcagcagac ccagcagccc aaggtcgacc
     3001 tgacggtgga cgccaagggc gggcgtgtga tgcgcgacgt cagccgcgag aacctgaaga
     3061 agcgcatggc catcgtgctg ttcgagaagg gcaagggcga ggtgctgacg gcgccggtga
     3121 tccaggccga gctgggcacg cgcttccaga tttccggctc gatgaccgtg agcgaggcca
     3181 acgacctggc gctgctgctg cgcgccggct cgctggcggc gccgatggag atcatcgagg
     3241 agcgcaccat cggccccagc ctgggcgccg acaacatcag caagggtttc cacagcgtga
     3301 tgtggggctt tctggccatc gtggtgttca tgatcgccta ctacatgctg ttcggcgtgt
     3361 tttcggccgt tgcgctgagc gtcaacctgc tgctgctggt ggccattctg tcgatgctgc
     3421 aggccacgct gacgctgccg ggcatggcgg cggtggcgct gacgctgggc atggcgatcg
     3481 acgccaacgt gctgatcaac gaacgcgtgc gcgaggagct gcgcggtggc gcctcgccgc
     3541 aggcggccat ccatgccggc tacgaccgcg cctgggccac catcctggac tccaacgtca
     3601 cctcgctgat cgccggcctg gcgctgctgg cgttcggcac cggcccggtg cgcggctttg
     3661 ccgtggtgca ctgcatcggc atcctgacct cgatgtactc ggcggtgttt ttctcgcgcg
     3721 gcctggtcaa cctgtggtat gggcggcaga agaagctcaa gtcggtgtcg atcggcaccg
     3781 tgtggcgacc cgcccccgag aaggtgtgag cgcgccgcaa ccgccgtcag acaagcaagg
     3841 aacaggcaga tggaattctt caggatcgga cgggacatcc cgttcatgcg ctatgcgcgg
     3901 cttttcaacg ccatatcgat cgtcaccttc ctgctggcgg tggtgtttct gttcacgcgt
     3961 ggcctgcacc tgtcggtcga gttcaccggc ggcacggtga tggaggtggc ctacagccag
     4021 ccggccgatc tggcgcaggt gcgtggcacg gtggcggcgc tgggttacgg cgacgtgcag
     4081 gtacagaact tcggcaccgc gcgcgacgtc atcatccggc tgccggtgca gcagggcaag
     4141 agctcggccc agcagagcga gcaggtgctg gccgcgctgc gcagcgccag ccccgacgtg
     4201 cagttgcgcc gcaccgaatt cgtcggcccg caggtcggcg acgagctggc gcgcgacggc
     4261 ctgtacgcgc tggccttcgt ggtggtcggc atcatgatct acctgtcgat gcgcttcgaa
     4321 tggaagtacg ccgtggccgg catcatcgcc aacctgcacg acgtggtgat cattctgggc
     4381 ttcttcgccc tgttccagtg ggagttctcg ctctcggtgc tggcggcgat cctggccgtg
     4441 ctgggctatt cggtcaacga atcggtggtg atcttcgacc gcatccgcga gaacttccgt
     4501 cgctaccgca agatgagcac ggtggagatc atcaacaact ccatcaccgc caacatcagc
     4561 cgcaccatca tcacccacgg ctcgacgcag atcatggtgc tgtcgatgtt gctgttcggc
     4621 ggcgagacgc tgcattactt cgccgtggca ctgaccatcg gcatcctgtt cggcatctat
     4681 tcctcggtgt tcgtggccgc cgccatcgcc atgtggctgg gcatccggcg cgaggacctg
     4741 gtcaagaccg gccgcaagga tggcgacccc ggtgacccga acgccggcgc ggtggtgtaa
     4801 gacagcggcc ctggattcca cgccgggcgt gccatactga ggggccacaa ccgctcccat
     4861 ggtttcgacg ccaccaccac cgtcgcctca tcgcctcgcg cagcaggtgc gcgagcgctt
     4921 cctgcaggtg ctcgacgcag ccgtcatcga gctgggtgag cgaatccaga ctcatctgac
     4981 gggccaggcg caaggcggga tccgcggcgc gacgctggcc gacatgcagc gtgccatgga
     5041 ggccgaggca gcgtttcggc gtgcgcgcac cgcctgggtg acggcggcac ggcgccactg
     5101 gcgcgatgca ctgacgcaga tgacggacag ccgcgccgcg cgtcgcagtc tgccgggtga
     5161 tctgggtctg gtggacgacg acagtgtcga gcgcaagatc gccgcctcgc gactgggggc
     5221 ggccgtgcag gacgctgctg gcgccgaatt caacgatctg cggctgcgca tgcaggcgct
     5281 cgagcgcagc accgacctgc cggcgcgcga cgtgctgcgg cccgacacct ttgcacagca
     5341 gttgctcaag gcctggcggg acgccggcct ggatcgtccg atgtgggtac tggttcaccc
     5401 ggagatccag gcggcaatga tcgaacgctt ggtgccactc tacgggcagg ccaatgcttt
     5461 cctgatcgag cgcggggtgc tggccgaaat cgacctgaag gcgctggtgc ggcgtggtga
     5521 tggcgatgcc gggcctgtcg caggtgctcg agcgtcggcg gttgcgggtg cgcgcgatcg
     5581 cgcagcgccc ggacccgcgg aaaatgccgc ttcccgggaa tcgggcgcgc gctcaggcgg
     5641 tgccggactg ttcggcgatg gccggtcggc ccgtggcgac gcgtttacca cccatggccg
     5701 ggccaatgcc gcggatgaca cccagacacc cgacgtgcgg cactccgcgc tggcgcgcgc
     5761 agcccaggaa acccgcatga tgacggccat ctcgcccatg acgcgcgtac ggcaacgagc
     5821 gcatggcgtg ctggggcagc tgcgacggct gctgaccgat cagttggcga acttcgacct
     5881 ggcggcgccg gcagagccac cttcgccgcc gttggctcgc gccatggccg aggcggcgcc
     5941 tttcgcagtc accgagttgc tggaccgcag cgacgccgac gtgaccatcg tgcaacccgt
     6001 ggccagtgtc gaggcggtgg cgacgcgtct gcgccagcga gccaccgagc tgaaggccaa
     6061 ggcggagcga ccgagcgaaa aggccaccat cgagatcgtg gccctgatgt tccaggccat
     6121 cctggccgaa gagcgcatcg cggcggcgat ccgcgtctgg ttcgcgcgcc tgcagattcc
     6181 ggtgttgcgg ctggcgttgg ccgagccgga cttcttcgcc tccatccagc acccggcgcg
     6241 ccagttgatc gaccgcatgg gtgcctgcgc gctgggcttc gacgccgcgc aggtcggcgg
     6301 cagccgcctg gagcgcgaga tcaagcgcat cgtgcaggtg atcgagcaat accctgaaac
     6361 cggccgccgc gtctaccagc tggtgctgga agagttcaag aagtttctcg gtcgctcact
     6421 gaccgaaggc ggctccgcac agcagatggc gacgctggcg cagcagatcg agcagaagga
     6481 agcgctgacc gtgcagtaca ccatcgagct gcggcgcatg ctggagccgg tgcccgtgcc
     6541 cgagccggtg cgcgagttcc tgttccgcgt ctggtgcgag gtgctggcca tggccgcggt
     6601 gcggcgcggg ccgcaggcgc ccgacacgct gcgcttcaag caggcggcgg ccgatctgct
     6661 gtgggcggtc agccccaagg ccgaccgcag cgaacgcggc cgcgtggtgc agcagctgcc
     6721 gacgctgttg cacgccctgc gcgagggcat ggaaggcctg gcgctgtcga cctcggacca
     6781 ggatgcccac atcaaggtgc tgaacgacgc ggtggtgcag gccttcgtct cgcgcggcga
     6841 gggcctgtcg gcggagcggc tggccgagct gtcgcgcaac ctggccggtc tggaagacgt
     6901 ggtcagcgac gacgcccagg gcgacgtgct gctcgacccg ggcgtgatcg agctgatgtt
     6961 cggcgccgaa ggcgacgcgc tggaggtgat tgccagcggc ggctcacagc ccggcgaggg
     7021 catgctgcac tgggccggcg agctggagct cggggcctgg ttcacgctcg actacctggg
     7081 cagcgtgacc caggtgcagt acgtgtggcg cagcaagcgt ggccagctgc acctgttcgg
     7141 cgacggctcg ggcaagagct acctggtgca gacctggcgc ctggcctcgt acctgcaggc
     7201 cggcctgctg gtgccggtgg aggacgaaac cctgaccgtg cgcgccacgc gcgaagccct
     7261 ggcgcggctc aacgccgagc ccgatcggct gctcaactga ccctggtgca gtgtttcgcg
     7321 ccgtcagg
//
LOCUS       DAOOOG010000127         7303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_12076_length_7303_cov_30.628587, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000127 DAOOOG010000000
VERSION     DAOOOG010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12076_length_7303_cov_30.628587"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PK242_09650"
     CDS             complement(<1..848)
                     /locus_tag="PK242_09650"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HOZ94323.1"
                     /translation="MKVIVLGGGVIGVSTAYYLARAGAEVTLLERQDDVALETSFANA
                     GQVSPGYSTPWAAPGIPLKAVKWLFQKHAPLSIRPDGTLFQLRWMAHMLRHCTSARYA
                     VNKERMTRLAEYSRDCLRQLRADTGIEYEQRSLGTLQVFRTAAQLQAARRDTAVLQEC
                     GVPYRLMTDVAELAEYEPALARARGLVGGLQLPGDETGDCRRFTTALAEQARALGVRF
                     RLGVQIAELLPRGGQIGGVRLASGEVLSADRYVLALGSCSRALLAPLGLDLPVYPVKG
                     YSLTVPL"
     gene            978..1460
                     /locus_tag="PK242_09655"
     CDS             978..1460
                     /locus_tag="PK242_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOZ94324.1"
                     /translation="MSSIDRIDRKILDQLQRDGRISMTELAQQVGLSTSPCAERVRRL
                     ERSGVIQGYHARVNPEALGRGLLVFVELTLSSKSAEVFRQVRDELLLEPRVLECHLVS
                     GTFDYLVKARLSAMREYRDLLGQILRKMPVPAQSNSYVVMEEIKEGLALPVDPVPVGA
                     "
     gene            complement(1496..2689)
                     /locus_tag="PK242_09660"
     CDS             complement(1496..2689)
                     /locus_tag="PK242_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94325.1"
                     /translation="MSTPFLRRLVMPATLCLAAALSACGGGGDDDATPPPPAPPGQAS
                     GSLLSSAPVNTWSAEQIAQALSDPDSKVQSGVTPRYAVRSHRVTYVSSDKHGQPVTAS
                     GLISVPLKPPGARSPVLSYQHATTFHDDQAPSNKVEAVEPPIVLASLGYIVLSADYVG
                     FGAAKGLEHPYLTAAPSAAVVLDLLHAGQTWRQQQAVADNGQLFLAGYSEGGYVTLAT
                     QRAIETTGDALRQQLQASVPGAGPYDVQATLDAQVEAVRAYNSTLGKLFEPHRLSQAP
                     EWVRNELRRLLLRQMVPDDADVRYQTLFLDRYMADDQAAIAAEHSVHLGWAPAVPVYL
                     FHGRDDRTVPYAAATSALNTLRAAGARDISLTDCTTPDYGHLKCVPEYFRFAVERMGR
                     LARDL"
     gene            2889..3509
                     /locus_tag="PK242_09665"
     CDS             2889..3509
                     /locus_tag="PK242_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ94326.1"
                     /translation="MSAADAMVYIVDDDAEVRAALAWLLRSRRLCSEGFDSAQAFEQA
                     LGEAPPQTPACLLLDVRMPGTSGLALFDRLLERGIATAWPVIFLTGHASVATAVEAVK
                     RGAFDFCEKPFSDNALVDRVERALAVSRERVAQLGAQRALRARLDELTEREREVLQRV
                     ASGRPNKLIADELGISVRTVEVHRARVFEKMDVKSAVELAQLLQQI"
     gene            complement(3547..3672)
                     /locus_tag="PK242_09670"
     CDS             complement(3547..3672)
                     /locus_tag="PK242_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94327.1"
                     /translation="MTLILLEALGALLLFVLLVWWTMFAGRKKGELPEPADDDTE"
     gene            3671..4828
                     /locus_tag="PK242_09675"
     CDS             3671..4828
                     /locus_tag="PK242_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOZ94328.1"
                     /translation="MQRVSRILAAAAMAALLAACALRPPPAPAPLPPAEPGLPPAGPV
                     ADTAPLGPPIERARSRWVPVRWAELPGFEQDDTAAAWNAWLKSCERPGPTFARLCSEV
                     RRLALGSTQQQRDWMRARLQPYRVEPLAGAPAGDGLLTSYFEPVYEASRVARPGFGAP
                     VYRAPPGASQRKPWYTRQELDTLPEARAQLAGHELVWLADPLDALILQIQGSGRVRVQ
                     EADGSQRLLRLAFAGTNEHPYRSPGKWLIEQGLLRGDATWPGIRAALAAQPQHLQGFL
                     WSNPRVVFFREEPLSALEAQFGPRGAQGVALTPGRSIAVDPQSIPYGTPVWLSSQGPV
                     AQLQRLVLAQDTGSAIVGAVRADYFAGWGAEAGELAGRMKQPLRLWVLWPR"
     gene            4909..5403
                     /locus_tag="PK242_09680"
     CDS             4909..5403
                     /locus_tag="PK242_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94329.1"
                     /translation="MAALPARSAPEATLFIAFRRLAGGLALAALGGCASWQDAPRLAY
                     QCPHELRFEARLYQDMALLEGLRGHAVLERVPGAEGDALHYADPTVRARFGLGLDARL
                     VRLDYTGIPEPVYCQHLPGADAPAAAVRASPRLGPRPPPPRPDPDAPVQTNIRQGDGR
                     NGPG"
     gene            complement(5414..5908)
                     /locus_tag="PK242_09685"
     CDS             complement(5414..5908)
                     /locus_tag="PK242_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOZ94330.1"
                     /translation="METLDAHDLAILEQLQADGRLTNAELAQRVGLSAAPCWRRVRAL
                     EQAGFIRGYRAEIDRHKLGLGVLAFVRLDAAQVSGAALRALEAAIRTRPEIVSCHYIS
                     GTGTFELQVVSTDLEGFSRFAREVLLNLPNVKDMHTSFSLGEVKAAGALPLEHLRAKP
                     RRGG"
     gene            6064..>7303
                     /locus_tag="PK242_09690"
     CDS             6064..>7303
                     /locus_tag="PK242_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOZ94331.1"
                     /translation="MNAPLPEHIRKALETVTLDDKYSLEHGRAFMSGIQALVKLPMLQ
                     RQRDALAGKNTAGFISGYRGSPLGGYDQALWAARKHLQGQHIVFQPGVNEELAATAVW
                     GTQQLGFAPPGSQRFDGVFGIWYGKGPGVDRSVDVFKHANMAGTTPWGGVIAVAGDDH
                     LAKSSTAAHQSDHIFKACGFPVFFPASVQDILDLGVHAFAMSRYAGVWAGMKTIQEIV
                     ESSASALIDPERVQIALPTDFDMPPGGVHIRWPDAALPQEERLFNTKWYAALAYVRAN
                     RLNRNVIEGPNDRLGLIASGKAYNDTRQALLDLGLDDAACRQLGIRLHKVAVVWPLEA
                     QITRQFAAGLREILVIEEKRQVIEYQLKEQLYNWRADVRPNVLGKYREGAAGDYSGGE
                     WSLDNPTANVLLRATADLSPA"
BASE COUNT         1066 a         2587 c         2620 g         1030 t
ORIGIN      
        1 agcggcacgg tgagcgaata gcccttgacc gggtacaccg gcagatcgag ccccagcggc
       61 gccagcagcg cccgcgagca gctgcccagg gccagcacgt agcggtcggc gctcagcacc
      121 tcgccgctgg ccagccgcac gccgccgatc tggccgccgc gcggcagcag ctcggcgatc
      181 tgcacgccca ggcggaaccg cacgcccagg gcgcgcgcct gttcggccag cgcggtggtg
      241 aagcgccggc agtcgccggt ttcgtcgccc ggcagctgca ggccgccgac caggccgcgc
      301 gcgcgcgcca gcgccggttc gtattcggcc agctcggcta cgtcggtcat caggcggtag
      361 ggcacgccac actcctgcag cacggcggtg tcgcgccggg ccgcctgcaa ctgcgccgcg
      421 gtgcggaaca cctgcagcgt gcccaggctg cgctgctcgt actcgatgcc ggtgtcggcg
      481 cgcagctggc gcaggcagtc gcggctgtat tcggccaggc gcgtcatgcg ctccttgttg
      541 acggcgtagc gcgcgctggt gcagtggcgc agcatgtggg ccatccagcg cagctggaac
      601 agcgtgccgt ccgggcggat cgacagcggc gcgtgcttct ggaacagcca cttgacggcc
      661 ttgagcggaa tgccgggcgc ggcccagggc gtggaatagc cgggcgacac ctgaccggcg
      721 ttggcaaaac tggtttccag cgccacgtcg tcctggcgct ccagcagggt gacttcggcg
      781 ccggcgcgcg ccaggtagta cgccgtgctg acgccgatca cgccgccgcc cagaacgatg
      841 accttcatgc ctgtctccat gatcctgtgg gaatgccgga agtctagggc ggggaggggc
      901 acaaaagtca ctgaaatgac ttgctcgcgg ctactgagtc agtgaaaatc actgaatcaa
      961 catcgtttgg cagtgaaatg agctccatcg atcgcatcga ccgcaaaatc ctcgaccagc
     1021 tgcagcgcga tggccgcatc tcgatgaccg agctggcgca gcaggtgggg ctgtccacct
     1081 cgccctgtgc cgagcgggtg cggcggctgg agcgcagcgg cgtgatccag ggctaccacg
     1141 cgcgcgtcaa ccccgaggcg ctggggcgcg ggctgctggt gttcgtggag ctgacgctgt
     1201 cgtccaagtc ggccgaggtg ttccgccagg tgcgcgacga gttgctgctg gagccgcgcg
     1261 tgctggaatg ccatctggtg tcgggcacct tcgactacct ggtcaaggcg cgcctgtcgg
     1321 cgatgcgcga gtaccgcgac ctgctgggcc agatcctgcg caagatgccg gtgccggcgc
     1381 agagcaacag ctacgtggtg atggaggaga tcaaggaggg gctggcgctg ccggtcgacc
     1441 cggtgccggt gggcgcctag cgtgctcacc caagaatcca taggttcaag ggtcgtcaca
     1501 ggtcgcgcgc cagccgcccc atgcgctcga ccgcgaagcg gaagtattcc ggcacgcatt
     1561 tcaggtggcc gtagtccggt gtggtgcagt cggtcaggct gatgtcgcgc gcaccggcgg
     1621 cgcgcagggt gttgagcgcc gaagtggccg ccgcgtaggg cacggtccgg tcgtcgcggc
     1681 cgtggaacag gtacaccggc acggcgggcg cccagcccag gtgcacgctg tgctcggcgg
     1741 cgatggcggc ctggtcgtcg gccatgtagc ggtcgaggaa cagcgtctgg tagcgcacgt
     1801 cggcgtcgtc cggcaccatc tggcgcagca gcaggcggcg cagttcgttg cgcacccatt
     1861 cgggcgcctg cgacaggcga tgcggctcga acagcttgcc cagcgtgctg ttgtaggcgc
     1921 gcacggcctc gacctgcgcg tccagcgtcg cctgcacgtc gtaagggccg gcgcccggca
     1981 cgctggcctg cagctgctgg cgcagcgcgt cgcccgtggt ttcgatggcc cgctgcgtcg
     2041 ccagggtgac gtagccgcct tccgaatagc cggccaggaa cagctggccg ttgtcggcca
     2101 cggcctgctg ctggcgccag gtctggccgg cgtgcagcag gtccagcacc accgcggcgc
     2161 tcggcgcggc cgtcaggtag gggtgctcca ggcccttggc ggcaccgaaa cccacgtaat
     2221 cggccgacag cacgatgtag cccagcgagg ccagcacgat cggcggctcc accgcctcca
     2281 ccttgttgct gggcgcctgg tcgtcgtgga aggtggtggc gtgctggtag ctcagcaccg
     2341 ggctgcgggc gccgggcggc ttgaggggca cgctgatcag ccccgacgcc gtgaccggct
     2401 ggccgtgctt gtcgctgctg acgtaggtca cacggtgcga gcgcaccgcg tagcgcggcg
     2461 tgacgccgct ctgcaccttg ctgtcggggt cggacagcgc ctgcgcgatc tgctcggccg
     2521 accaggtgtt gaccggcgcg ctcgacagca ggctgccgct ggcctgaccc gggggcgcag
     2581 gcggcggcgg ggtggcgtcg tcgtcgccgc cgccgccgca ggcgctcagg gccgcggcca
     2641 ggcacaaggt ggccggcatg acgagacggc gcaggaacgg ggtggacatg cagaactctc
     2701 ccagatgaac gaggccggat gttacggcca gccggccagg cccagccaag ccgagcttcg
     2761 acagcctcct gtggattcgg cctccgggat gtcttgagca ccgttcgggc cgagcctgtc
     2821 gcagcccttg gacaggctca gagcgaacgg cgcgatggtc acgattccga atccatagaa
     2881 tccgccgcat gtccgctgcc gatgccatgg tctacatcgt cgacgacgat gccgaggtgc
     2941 gcgcggcgct ggcctggctg ctgcgctcgc gccggctgtg ctcggaaggc ttcgacagcg
     3001 cacaggcctt cgagcaggcg ctgggcgagg cgccgccgca gacgccggcc tgcctgttgc
     3061 tcgacgtgcg catgcccggc accagcgggc tggcgctgtt cgatcgcctg ctggagcgcg
     3121 gcatcgccac cgcctggccg gtgatctttc tgaccggtca cgccagcgtg gcgacggcgg
     3181 tggaggcggt caagcgcggc gccttcgact tctgcgagaa gccgttttcc gacaacgcgc
     3241 tggtcgatcg cgtcgagcgc gcgctggcgg tgtcgcgcga gcgcgtggcg cagctcggtg
     3301 cgcagcgtgc gctgcgcgcg cgcctggacg agctgaccga gcgcgagcgc gaggtgctgc
     3361 agcgggtggc cagcgggcgc cccaacaagc tgatcgccga cgagctgggc atcagcgtgc
     3421 gcacggtgga ggtgcaccgc gcccgggtgt tcgagaagat ggacgtcaaa tcggccgtcg
     3481 agctggctca gttgctacaa caaatatagc tatcggcgct tgccaggcgg gagccagagg
     3541 ccaatttcat tcggtatcgt cgtcggccgg ctcgggcagc tcgcccttct tgcggccggc
     3601 gaacatggtc caccacacca gcagcacgaa cagcaacagg gcgcccagcg cttccagcag
     3661 aatcagggtc atgcaacgag tatcacggat tctggcggcg gcggccatgg ccgctctgct
     3721 ggccgcctgc gcgctgcgcc cgccgccggc gcccgccccg ctgccgccgg ccgagcccgg
     3781 tctgcccccc gccggcccgg tggccgacac ggcgccgctg ggcccgccca tcgagcgcgc
     3841 gcgcagccgc tgggtgccgg tgcgctgggc cgagctgccc ggcttcgagc aggacgacac
     3901 cgccgccgcc tggaacgcct ggctcaagag ctgcgagcgc cccggcccca cctttgcccg
     3961 tctgtgcagc gaggtgcggc gcctggcgct gggcagcacg cagcagcagc gcgactggat
     4021 gcgcgcgcgc ctgcagccct accgcgtcga gcccctggcc ggcgcgccgg ccggcgacgg
     4081 cctgctgacc agctacttcg agccggtgta cgaagcctcg cgcgtggcgc gcccgggctt
     4141 tggcgcgccg gtgtaccgcg ccccgcccgg cgccagccag cgcaagccct ggtacacgcg
     4201 ccaggagctc gacaccctgc ccgaggcgcg cgcccagctg gccggccacg agctggtctg
     4261 gctggccgat ccgctggacg cgctgatcct gcagatccag ggctcgggcc gggtgcgcgt
     4321 gcaggaggcc gacggcagcc agcgcctgct gcggctggcc tttgccggca ccaacgaaca
     4381 cccttaccgc agccccggca agtggctgat cgagcagggg ctgctgcgcg gcgacgccac
     4441 ctggcccggc atccgcgccg cgctggcggc gcagccgcag cacctgcagg gctttctgtg
     4501 gagcaacccg cgcgtggtgt tcttccgcga ggagccgctg tcggcgctgg aggcgcagtt
     4561 cggcccgcgc ggcgcgcagg gtgtggcgct gacgccgggg cgctcgatcg ccgtcgaccc
     4621 gcagagcatt ccctacggca cgccggtgtg gctgtcctcg caggggccgg tggcgcagct
     4681 gcagcgcctg gtgctggcgc aggacaccgg cagcgccatc gtcggcgccg tgcgcgccga
     4741 ctactttgcc ggctggggcg ccgaggcggg cgaattggcc ggccgcatga agcagccgct
     4801 gcgcctgtgg gtgctgtggc cccggtgagc cgcgcccgcg cgcccgaagc gggccgccgc
     4861 gccgcctggg cgggcgccgc gcgccccgcc tcgatattca aagcaaaaat ggctgcgctg
     4921 cccgcccgat ctgcaccaga agctacgtta tttatagcat tcaggcggct cgccggcggc
     4981 ctggcactgg ccgcgctggg cggctgcgcg agctggcagg atgcgccacg cctggcctac
     5041 cagtgcccgc acgagctgcg cttcgaggcc cggctgtacc aggacatggc cctgctcgaa
     5101 ggcctgcgcg gccacgccgt gctcgagcgc gtgcccggcg ccgaaggcga cgcgctgcac
     5161 tatgccgacc ccacggtgcg ggcgcgcttc ggcctcggtc tggacgcgcg cctggtgcgg
     5221 ctggactaca ccggcatccc cgagccggtg tactgccagc acctgcccgg cgccgacgcc
     5281 ccggcggcgg ccgtgcgcgc cagcccgcgc ctcggcccgc gcccgccgcc gccgcgaccc
     5341 gaccccgacg ccccggtgca gaccaacatc cggcagggcg acggtcgcaa cgggccgggt
     5401 tgagggcggg caactaaccg ccgcggcgcg gcttggccct gaggtgctcc agcggcagcg
     5461 cgccggccgc cttgacctcg cccagcgaga agctggtgtg catgtccttc acgttgggca
     5521 ggttcagcag cacctcgcgc gcgaagcggc tgaagccttc caggtcggtg ctcaccacct
     5581 gcagctcgaa ggtgccggtg ccgctgatgt agtggcagct gacgatttcg gggcgggtgc
     5641 ggatcgccgc ctccagcgcc cgcagcgccg cgccgctgac ctgggccgca tccagccgca
     5701 cgaaggccag cacgcccagg ccgagcttgt ggcgatcgat ttcggcgcgg taaccgcgga
     5761 tgaagccggc ctgctccagc gcccgcacgc gccgccagca gggtgcggcc gacaggccga
     5821 cgcgctgcgc aagttcggcg ttggtgagcc ggccgtcggc ctgcagttgt tccaggatgg
     5881 ccagatcgtg ggcgtcaagt gtttccatgg tgggtggatt ctagaaagaa tctttcctga
     5941 acaccgtgga tcgcggcaca taaagaaagc acctgtcgcg tcagccgacc tacactttgc
     6001 cgacccgaca cacaggcggc tgccggccat cggccaggcg gcccgacaca ggagaccacc
     6061 ccgatgaacg ccccgctgcc cgaacacatc cgcaaggcgc tggaaaccgt cacgctggac
     6121 gacaaataca gcctggagca cggccgcgcc ttcatgagcg gcatccaggc gctggtcaag
     6181 ctgccgatgc tgcagcgcca gcgcgatgcg ctcgccggca agaacaccgc cggcttcatc
     6241 agcggctacc gcggctcgcc cctgggcggc tacgaccagg cgctgtgggc cgcccgcaag
     6301 catctgcagg gccagcacat cgtgttccag cccggcgtca acgaagagct ggccgccacc
     6361 gccgtctggg gcacgcagca gctgggcttt gcgccgcccg gcagccagcg cttcgacggc
     6421 gtgttcggca tctggtacgg caaggggccg ggggtggacc gcagcgtcga cgtgttcaag
     6481 cacgccaaca tggccggcac cacgccctgg ggcggcgtga ttgccgtcgc cggcgacgat
     6541 cacctggcca agagcagcac cgccgcgcac cagagcgacc acatcttcaa ggcctgcggc
     6601 tttccggtgt tctttccggc cagcgtgcag gacatcctgg atctgggcgt gcacgccttc
     6661 gccatgagcc gctacgccgg cgtgtgggcc ggcatgaaga cgatccagga gatcgtcgaa
     6721 tccagcgcca gcgccctgat cgaccccgaa cgggtgcaga tcgcgctgcc gaccgacttc
     6781 gacatgccgc ccggcggcgt gcacatccgc tggcccgacg ccgcgctgcc gcaggaggag
     6841 cgcctgttca acaccaagtg gtacgcggcc ctggcctatg tgcgcgccaa ccgcctgaac
     6901 cgcaacgtca tcgaagggcc gaacgaccgc ctgggcctga tcgccagcgg caaggcctac
     6961 aacgacaccc gccaggcgct gctggacctg gggctggacg acgccgcctg ccgccagctc
     7021 ggcatccgcc tgcacaaggt ggcggtggtg tggccgctgg aggcgcagat cacgcgccag
     7081 ttcgccgccg gcctgcgcga gatcctggtg atcgaggaaa agcgccaggt catcgaatac
     7141 cagctcaagg agcagctcta caactggcgc gccgacgtgc ggcccaacgt gctgggcaag
     7201 taccgcgagg gcgccgcggg cgactacagc ggcggcgaat ggtcgctcga caaccccacg
     7261 gccaacgtgc tgctgcgcgc caccgccgac ctgtcgccgg cgc
//
LOCUS       DAOOOG010000128         7297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_12083_length_7297_cov_29.971141, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000128 DAOOOG010000000
VERSION     DAOOOG010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12083_length_7297_cov_29.971141"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="PK242_09695"
     CDS             complement(<1..1772)
                     /locus_tag="PK242_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOZ94332.1"
                     /translation="MSPTDDRPIQPQRYGSGQAVRRLEDDALLQGQGRFTDDLGAPGD
                     GWLVFVRSPHAHAALRSVDAEAARALPGVRAVITGADLVAAGMPPLPTGAPFRRADGS
                     ACATPPRRALAHEVVRHVGEAVAAVVADSLAQARDAADALVVDYRELPVVASLAAALR
                     ADAVVTPAAPDNIACEARHGDAAAAQAAFAQAAHVVALDLTHQRLAALTLEPRAVRAA
                     WDAARGRLDMHASTQMPTALRATVASALGLTPEAVRVRVGDVGGGFGMKTGAYPEDVV
                     VAWAARHTGRPVRWSGERGEEFLSTTHGRDVQAHAELALDAEGRMLALRVHSHANVGA
                     YALATGVAIQLLIGPWVQTSVYHVPVIDFHFRAVLTHQAPTGAYRGAGRPEAIFNIER
                     LLDEAARQSGIDRIALRRRNFIRPEQMPYTNPMGQTYDVGRFERVLDQALPLADWDGF
                     AARAQQSRARGLWRGLGIATFLEWTGGNAFEESVTLEVKSDGIVEVFSAVNQMGQGIA
                     TSLAQLVVDVLQVPIERVRVVLGDTERGNGFGSAGSRSLFTGGSAMRGGAEHTLAQAH
                     QLAASALEAAPEDLRYQAARFHVVG"
     gene            1985..5467
                     /gene="mfd"
                     /locus_tag="PK242_09700"
     CDS             1985..5467
                     /gene="mfd"
                     /locus_tag="PK242_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOZ94333.1"
                     /translation="MHLPKLIPGKRHALPRPPASSDALLLARLAQREAAEQRLVAIVT
                     ADAGDARRLLDELGFFAPDLRVVLFPDWETLPYDTFSPHQDLISERLATLWAIRQGQA
                     DVVLLPATTALYRLAPPAFLAAHTFQFRQGQRLDEAALRSQLTLAGYQHVSQVVSPGE
                     YAVRGGLIDLYPMGSPMPYRVDLFDDEIDSIRGFDPDTQRSLYPVPEVRLLPGREFPL
                     DEAARTRFRQRWRELLEGDPTRSRIYKDMAGGVATAGIEYYLPLFFDHTATVFDYLGS
                     GATLVLHGELETSFQQFSQDTRERWRLAQGDPERPVLPPEALFLNAEQFYLQAKACPQ
                     VALRPGVDDIEHSAFAQKLPPLTVVRGADDPLARLQAHLRGAARRLLVLAESDGRRQS
                     LLDFLRASGLNPPAFDTLAEFEASTEPVGIATAALAEGFAWVEGGIDFVTETELFATA
                     PGTRRRRRQEQVSDVEALIKDLSELNVGDPVVHTQHGIGRYRGLVHLDMGEKNADGTP
                     ALQEFLHLEYAQQAVLYVPVSQLHLIGRYTGVSADEAPLHRLGSGQWDKARRKAAEQV
                     RDTAAELLNLYARRAARQGHAFRYSPHDYEQFAADFGFEETADQQAAIHAVIQDLISP
                     RPMDRLVCGDVGFGKTEVALRAAFVAVTGGKQVAFLAPTTLLAEQHYQTLVDRLARWP
                     IKVAQMSRFASGKETAAALKGLADGSVDIVVGTHKLLSEKTQFKNLGLLIIDEEHRFG
                     VRHKEAMKKFRAEVDVLTLTATPIPRTMGMALEGLRDLSVIATAPERRLAIKTFVRDE
                     GAGVIREAVLRELKRGGQVYFLHNEVETIENRRQALEKILPEARIAIAHGQMPERELE
                     RVMRDFVAQRFNLLLCSTIIETGIDVPTANTIVIARADRFGLAQLHQLRGRVGRSHHQ
                     AYAYLLVPDQQSLTKQAAQRLEAIQQMEELGSGFYLAMHDLEIRGAGEVLGENQSGNM
                     MEIGFQLYNEMLAEAVRSLKAGREPDLLAPMQAATDINLHAPALLPHDYCGDVHLRLS
                     FYKKLATARTGEQIDALVEEIVDRFGKLPAPAQTLIDVHRLRVLSAPYGVQKVDAAPG
                     VISIAFKPNPPIDPMAIIHLIQKNRHIKLAGNDKLRIERALPDPQARAQLVRDVLRSL
                     GQPIQPPLAASAPA"
     gene            5486..6121
                     /locus_tag="PK242_09705"
     CDS             5486..6121
                     /locus_tag="PK242_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOZ94334.1"
                     /translation="MNPSWPSRRLWHQLLWLGALALLAAPALADEALARRFAAAALRA
                     GDTGGRAFAVVSKPAATVWVFDRHGQLLASSAVLLGQATGDAAPPDVGSRPLHQLRPH
                     EKVTSAGRFLTEAGRNHRGEDIVWLDYDAALSMHRVRQVAGEQRPQRLQTPGVADNRI
                     SYGCINVPDRFYDRTIDPLFARQSGVVYVLPETEPIERWFAFARDLDAAAP"
     gene            6195..6935
                     /gene="serB"
                     /locus_tag="PK242_09710"
     CDS             6195..6935
                     /gene="serB"
                     /locus_tag="PK242_09710"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HOZ94335.1"
                     /translation="MTSQTALDASNEISPGLVLRGIQPPLRLSDYRLIAFDMDSTLIN
                     IECVDEIADAVGRKAEVAAITEAAMRGEITDFKDSLRRRVALLRGVSVDELAQVYRAR
                     LRLNPGAAELVLACKAAGLKVLLVSGGFSYFADRLRDRLALDFARSNLLEIEHGRLTG
                     RIQPQSWGDICDGAMKRETVLSTCSLIGCSPSQAIAMGDGANDLEMMRAVGLSVAYRA
                     KPRVRAQARVAIDMGGLDRLLEVFGAQV"
     gene            7096..>7297
                     /locus_tag="PK242_09715"
     CDS             7096..>7297
                     /locus_tag="PK242_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94336.1"
                     /translation="MGLKQRAAQAGRWLAGVLFGVLGLTSMAGAQPVAVSSFAEGQGN
                     GVYAFASSTPKTLGELMQGGKEP"
BASE COUNT         1086 a         2681 c         2529 g         1001 t
ORIGIN      
        1 ccgacgacgt gaaagcgcgc cgcctggtag cgcaggtcct cgggcgcggc ttccagcgcg
       61 ctggccgcga gctgatgcgc ctgcgccagc gtgtgctcag cgccgccgcg catggccgag
      121 ccgccggtga acagtgagcg cgagccggcg ctgccgaagc cgttgccgcg ctcggtgtca
      181 cccagcacca cgcgcacgcg ctcgatcggc acctgcagca catcgaccac cagctgcgcc
      241 agcgaggtgg caatgccctg gcccatctgg ttgacggcgg agaacacctc gacgatgccg
      301 tccgacttga cttccagggt cacgctctcc tcgaacgcat tgccgccggt ccactccaga
      361 aaggtggcga tgcccagccc gcgccaaagc ccgcgcgccc tcgactgctg cgcgcgcgcc
      421 gcaaagccgt cccagtcggc cagcggcagc gcctgatcca gcacgcgctc gaagcggccg
      481 acgtcgtagg tctggcccat cgggttggtg tagggcatct gctcggggcg gatgaagttg
      541 cgccgccgca gggcgatgcg gtcgatgccg ctctggcgcg ccgcctcgtc cagcaggcgc
      601 tcgatgttga agatggcctc ggggcggccg gcgccgcgat aggccccggt gggcgcctgg
      661 tgcgtcagca cggcgcggaa gtgaaagtcg atcaccggca cgtgatagac gctggtctgc
      721 acccaggggc cgatcagcag ctggatcgcc acgccggtgg ccagcgcgta ggcgcccacg
      781 ttggcgtgcg agtgcacgcg cagcgccagc atgcggccct cggcgtcgag cgccagctcg
      841 gcgtgcgcct gcacgtcgcg gccgtgggtg gtgctgagaa actcctcgcc gcgctcgccg
      901 ctccagcgca ccggccggcc ggtgtggcgc gcggcccagg ccaccaccac gtcttcgggg
      961 taggcgccgg tcttcatgcc gaagccgccg ccgacgtcgc ccacgcgcac gcgcacggct
     1021 tcgggcgtca gcccgagcgc gctggccacg gtggcgcgca gcgccgtcgg catctgggtg
     1081 ctggcgtgca tgtccagccg gccgcgggcg gcatcccagg cggcgcgcac cgcgcgcggc
     1141 tccagcgtca gcgcggccag ccgctggtgc gtcagatcga gcgccaccac gtgcgccgcc
     1201 tgcgcaaacg ccgcctgcgc ggcggccgca tcgccatggc gcgcttcgca ggcgatgttg
     1261 tcgggcgcgg cgggcgtgac cacggcgtcg gcacgcaggg ccgccgccag cgaggccacc
     1321 accggcagct cgcggtagtc gaccaccagc gcgtcggccg catcgcgcgc ctgcgccagg
     1381 ctgtcggcca ccacggcggc caccgcctcg ccgacatggc gcaccacctc gtgcgccagg
     1441 gcgcgccgcg gcggcgtggc gcaggcgctg ccgtcggcgc gcctgaaggg cgcgccagtc
     1501 ggcagcggcg gcatgccggc ggccaccagg tcggcgccgg tgatgacggc gcgcaccccg
     1561 ggcagggcgc gcgcggcctc ggcatcgacg ctgcgcaggg cggcatgcgc gtgcggcgag
     1621 cgcacgaaca ccaaccagcc gtcgcccggc gcgcccagat cgtcggtgaa gcggccctgg
     1681 ccctgcagca gcgcgtcgtc ctccagccgg cgcacggcct ggccgctgcc gtagcgctgc
     1741 ggttggatgg ggcgatcgtc ggttggactc atgggctcgg ctcctgcggt ggcgggcagc
     1801 cactatagac cggccaccga aaagccgcgc cggccgctgc gctactatct tttttatagc
     1861 tgctggcgct tgtctggagc gcgccagagt ccaaaaacgt tcagattccg gagcggtaaa
     1921 ataggcccgt cacgcccccg cccgacccgg ccgggggttt ttgtcgtttc cgccgctgcc
     1981 cgccatgcac ctgcccaagc tcatccctgg caaacgccat gccctgcccc gcccgccggc
     2041 ctccagcgac gcgctgctgc tggcgcgact ggcgcagcgc gaggccgccg agcagcggct
     2101 ggtggccatc gtcaccgccg acgccggcga cgcgcggcgg ctgctggacg agctcggctt
     2161 tttcgccccc gacctgcggg tggtgctgtt tcccgactgg gaaaccctgc cctacgacac
     2221 gttctcgccg caccaggacc tgatcagcga gcgcctggcc acgctgtggg cgatccgcca
     2281 gggccaggcc gacgtggtgc tgctgccggc caccacggcg ctgtaccggc tggcgccgcc
     2341 ggcctttctg gccgcccaca ccttccagtt ccggcagggg cagcggctcg acgaggcggc
     2401 gctcagaagc cagctcacgc tggccggcta ccagcacgtc agccaggtgg tcagcccggg
     2461 cgagtacgcg gtgcgcggcg gtctgatcga cctctacccg atgggcagcc ccatgcccta
     2521 ccgggtcgat ctgttcgacg acgagatcga ctccatccgc ggcttcgacc ccgacaccca
     2581 gcgcagcctg tacccggtgc ccgaggtgcg gctgctgccg gggcgcgagt tcccgctcga
     2641 cgaggcggcg cgcacacgct ttcgccagcg ctggcgcgag ctgctggaag gcgatccgac
     2701 gcgcagccgc atctacaagg acatggcggg cggcgtggcc accgccggca tcgagtacta
     2761 cctgccgctg ttcttcgacc acacggccac ggtgttcgac tatctcggca gcggcgccac
     2821 gctggtgctg catggcgagc tggagacgag cttccagcag ttcagccagg acacgcgcga
     2881 gcgctggcgc ctggcccagg gcgatccgga acgcccggtg ctgccgcccg aggcgctgtt
     2941 cctgaacgcc gagcagttct atctgcaggc caaggcctgc ccgcaggtgg cgctgcgccc
     3001 gggcgtggac gacatcgagc acagcgcctt tgcccagaag ctgccgccgc tgaccgtggt
     3061 gcgcggcgcc gacgacccgc tggcgcgcct gcaggcgcac ctgcgcggcg ccgcgcggcg
     3121 cctgctggtg ctggccgaaa gcgacggccg gcgccagagc ctgctggact ttctgcgcgc
     3181 cagcggcctg aacccgccgg ccttcgacac gctggccgaa ttcgaggcca gcaccgagcc
     3241 ggtcggcatc gccaccgccg cgctggccga gggctttgcc tgggtcgaag gcggcatcga
     3301 cttcgtcacc gaaaccgagc tgttcgccac cgcccccggc acgcgtcggc gccggcggca
     3361 ggagcaggtc agcgacgtcg aggcgctgat caaggacctg tccgagctca acgtcggcga
     3421 cccggtggtg cacacccagc acggcatcgg ccgctaccgc ggcctggtgc acctggacat
     3481 gggcgagaag aacgccgacg gcacgccggc gctgcaggaa ttcctgcacc tggaatacgc
     3541 gcagcaggcc gtgctgtacg tgccggtgtc gcagctgcac ctgatcggcc gctacaccgg
     3601 cgtcagtgcc gacgaggcgc cgctgcaccg gctgggcagc ggccagtggg acaaggcgcg
     3661 gcgcaaggcc gccgagcagg tgcgcgacac cgctgccgag ctgctcaacc tgtacgcccg
     3721 acgcgccgcg cgccagggcc atgcgttccg ctattcgccg cacgactacg agcagttcgc
     3781 ggccgacttc ggtttcgagg aaaccgccga ccagcaggcc gccatccacg ccgtgatcca
     3841 ggacctgatc agcccgcggc cgatggaccg gctggtgtgc ggcgacgtcg gcttcggcaa
     3901 gaccgaagtc gctctgcgcg ccgccttcgt ggcggtcacc ggcggcaagc aggtggcgtt
     3961 tctggcgccc accacgctgc tggccgagca gcattaccag acgctggtcg atcgcctggc
     4021 ccgctggccc atcaaggtgg cgcagatgag ccgctttgcc tccggcaagg aaactgccgc
     4081 cgcgctcaag ggcctggccg acggcagcgt ggacatcgtg gtcggcacgc acaagctgct
     4141 gtcggaaaag acccagttca agaacctggg cctgctcatc atcgacgagg aacaccgctt
     4201 cggcgtgcgc cacaaggagg cgatgaagaa gttccgcgcc gaggtcgacg tgctcacgct
     4261 caccgccacg ccgatcccgc gcaccatggg catggcgctg gaaggcctgc gcgacctgag
     4321 cgtgatcgcc accgcgcccg agcgccgcct ggccatcaag accttcgtgc gcgacgaagg
     4381 cgcgggcgtg atccgcgagg cggtgctgcg cgagttgaag cgcggcggcc aggtgtactt
     4441 tctgcacaac gaggtcgaga ccatcgaaaa ccgccgccag gcgctggaaa agatcctgcc
     4501 cgaggcgcgc atcgccatcg cccacggcca gatgcccgag cgcgagctgg agcgcgtgat
     4561 gcgcgacttc gtggcgcagc gcttcaacct gttgctgtgt tcgaccatca tcgagaccgg
     4621 catcgacgtg cccaccgcca acaccatcgt catcgcgcgc gccgaccgct tcggcctggc
     4681 gcagctgcac cagttgcgcg gccgcgtggg gcgcagccac caccaggcct acgcctacct
     4741 gctggtgccc gaccagcaga gcctgaccaa gcaggccgcc cagcggctgg aggcgatcca
     4801 gcagatggag gagctgggtt cgggctttta cctcgccatg cacgacctgg aaatccgcgg
     4861 cgccggcgag gtgctgggcg aaaaccagag cggcaacatg atggaaatcg gctttcagct
     4921 ctacaacgag atgctggccg aggccgtgcg cagcctcaag gccgggcgcg agcccgacct
     4981 gctggcaccc atgcaggcgg ccaccgacat caacctgcac gcccccgccc tgctgcccca
     5041 tgactattgc ggcgacgtgc acctgcgcct gtccttctac aagaagctgg cgacggcgcg
     5101 caccggcgag cagatcgacg ccctggtgga agaaatcgtc gaccgcttcg gcaagctgcc
     5161 cgccccggcg cagaccctga tcgacgtgca ccgcctgcgc gtgctgagcg ccccgtatgg
     5221 ggtgcagaag gtggacgccg cgccgggcgt gatcagcatc gccttcaagc ccaacccgcc
     5281 gatcgatccc atggccatca tccatctgat ccagaagaac cgccacatca agctggccgg
     5341 caacgacaag ctgcgcatcg aacgcgccct gcccgacccg caggcgcgcg cgcagctggt
     5401 gcgcgacgtg ctgcgcagcc tcgggcaacc gatccagccg ccgctggccg caagcgcccc
     5461 ggcataggcc agaatggcgc cacccatgaa cccgtcctgg ccttcccgcc gcctctggca
     5521 ccagctgctg tggctgggcg cgctggcgct gctggccgcg cccgcgctgg ccgacgaagc
     5581 cctggcccgc cgctttgccg ccgccgcgct gcgcgccggc gacaccggcg ggcgcgcctt
     5641 cgcggtggtc agcaagccgg ccgccacggt ctgggtgttc gaccgccacg gccagctgct
     5701 ggccagcagc gccgtgctgc tgggtcaggc caccggcgac gccgcgcccc cggacgtcgg
     5761 cagccgcccg ctgcaccagc tgcgcccgca cgaaaaggtc accagcgccg gccgcttcct
     5821 caccgaggcg gggcgcaacc accgcggcga agacatcgtc tggctggact acgacgccgc
     5881 gctgtcgatg caccgcgtgc gccaggtggc gggcgagcag cgcccgcagc gcctgcagac
     5941 gcccggcgtg gccgacaacc gcatttcgta tggctgcatc aacgtgccgg atcgcttcta
     6001 cgaccgcacc atcgacccgc tgtttgcgcg ccagtcgggc gtggtctatg tgctgccgga
     6061 aaccgagccg atcgagcgct ggtttgcgtt cgcacgcgat ctggacgcgg ccgccccatg
     6121 acccctggat tcagcatcat tttggcctcc agcgcttgtc cagcaagcgc cagcagctat
     6181 tctttcagta gttcatgacc tcccagaccg cccttgacgc atcgaacgaa atcagccccg
     6241 gcctggtgtt gcgcggcatc cagcccccgc tgcgcctgtc ggactaccga ctgatcgcct
     6301 tcgacatgga ctccacgctg atcaacatcg aatgcgtgga cgagatcgcc gacgccgtgg
     6361 gccgcaaggc cgaggtggcc gccatcaccg aagcggccat gcgcggcgag atcaccgact
     6421 tcaaggacag cctgcgccgg cgcgtggcgc tgctgcgggg cgtgtcggtg gacgagctgg
     6481 cgcaggtcta ccgtgcccgc ctgcgcctga acccaggcgc cgccgagctg gtgctggcct
     6541 gcaaggcggc cgggctgaag gtgctgctgg tgtcgggtgg cttcagctac tttgccgacc
     6601 gcctgcgcga ccggctggcg ctggacttcg cgcgcagcaa cctgctggag atcgagcacg
     6661 ggcgcctgac cgggcgcatc cagccgcaga gctggggcga catctgcgac ggcgccatga
     6721 agcgcgagac ggtgctgtcc acctgcagcc tgatcggctg ctcgcccagc caggcgatcg
     6781 ccatgggcga cggcgccaac gacctggaga tgatgcgcgc cgtcggcctg tcggtggcct
     6841 accgggccaa gccccgcgtg cgcgcccagg ccagggtggc gatcgacatg ggcgggctgg
     6901 accggctgct ggaagtgttc ggcgcccagg tctgaggcgc cgcgcggccg cgcaacggcc
     6961 gccggcggca cacgacatcc gccaccaccc cgccgcgatc gacgcccggc gcgatcgcca
     7021 ttcgtcgttt tctgggtgcc attgcgcacg ggatgccgta cactgccgcc accgatgctt
     7081 cgatggaggc gcatcatggg tctgaaacaa cgcgcggcgc aggccggcag gtggctcgca
     7141 ggggtgctgt tcggtgtgct gggcctgacg tccatggcgg gggcgcagcc ggtggccgtc
     7201 agcagctttg ccgagggcca gggcaacggg gtgtatgcgt tcgccagctc gacgcccaag
     7261 acgctgggcg agctgatgca gggcggcaag gagccgg
//
LOCUS       DAOOOG010000129         7251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_12180_length_7251_cov_36.041829, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000129 DAOOOG010000000
VERSION     DAOOOG010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12180_length_7251_cov_36.041829"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PK242_09720"
     CDS             complement(<1..349)
                     /locus_tag="PK242_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOZ94337.1"
                     /translation="MKKKLSVLAFTLAIAGAAAPAFAQQQYAKAEDAVHYRRGAFTLL
                     GYHFGSLGAMVQGKVPFNSAQAAIDGDVIAIASKLPFHAFVEGSDKGQTGAKPEIWKE
                     QAKFTDLRDKMQAE"
     gene            568..1242
                     /locus_tag="PK242_09725"
     CDS             568..1242
                     /locus_tag="PK242_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOZ94338.1"
                     /translation="MHTVRIWDLPTRLFHWVLFVCVVALVVTAKVGGNAMNLHLRLGY
                     VVFTLLLFRLLWGFVGGHWSRFASFFPTPGRLTRYLGGRATTADTAGHNPLGALSVLA
                     MLLVLGAQVGSGLFSDDEIAFSGPLTALVSNATVGRLTEWHSHWGQYLVIGLVLLHVL
                     AIVVYRLRRQNLVGPMVVGDKTLPEPVTAARDSAGTRLLALALLGVAAALVYAVVQLG
                     SRGALG"
     gene            1312..1791
                     /locus_tag="PK242_09730"
     CDS             1312..1791
                     /locus_tag="PK242_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ94339.1"
                     /translation="MHLAPAREPAHYDAARALFREYADQLGVDLCFQGFEAELAGLPG
                     DYQEPGGTLLLAWVDGELAGCCALRPIEDVDYANAAEMKRLFVRKAFRGFGLGRQLAE
                     AVLEAAVRLGYDSVLLDTLDDMEAARALYEDLGFVEIPPYYHNPLPGAHYLKVELRV"
     gene            complement(1891..2400)
                     /locus_tag="PK242_09735"
     CDS             complement(1891..2400)
                     /locus_tag="PK242_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOZ94340.1"
                     /translation="MIKVGDALPAVTLSEYIEVEGNGCSIGPNPVEVQKAAAGKTIAV
                     FAVPGAFTPTCSAKHVPSYLQHAAALKAAGVDEIWCLSVNDAFVMGAWAREQKTAGQI
                     RMLADGDAAFAQATGLTLDLHGKGLGLRSNRYSMLVKDGKVAALNVEAPGKFEVSDGA
                     TLLAQLRAA"
     gene            complement(2543..2812)
                     /gene="rpsQ"
                     /locus_tag="PK242_09740"
     CDS             complement(2543..2812)
                     /gene="rpsQ"
                     /locus_tag="PK242_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOZ94341.1"
                     /translation="MTESQPSLKRTLIGKVVSDKRAKTVTVLVERRVKHPIYDKIVMR
                     SSKYHAHDENGQYKLGDTIEITESRPLSKTKNWVATRLVQKAAQV"
     gene            complement(2822..3019)
                     /gene="rpmC"
                     /locus_tag="PK242_09745"
     CDS             complement(2822..3019)
                     /gene="rpmC"
                     /locus_tag="PK242_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOZ94342.1"
                     /translation="MKTAELRQKDVAGLQAEVKELQKAHFNLRMQKGTQQLSNTSQLK
                     ATRRAIARAKTLIVEKQAAAK"
     gene            complement(3036..3452)
                     /gene="rplP"
                     /locus_tag="PK242_09750"
     CDS             complement(3036..3452)
                     /gene="rplP"
                     /locus_tag="PK242_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOZ94343.1"
                     /translation="MLQPARRKYRKEHKGRNTGIATRGNTVAFGDFGLKSTDRGRLTA
                     RQIESARRAISRHVKRGGRIWIRVFPDKPISTKPAEVRMGNGKGNPEYYVAEIQPGKV
                     LYEIVGVPEELAREAFALAAAKLPLRTTFVARMIGQ"
     gene            complement(3456..4352)
                     /gene="rpsC"
                     /locus_tag="PK242_09755"
     CDS             complement(3456..4352)
                     /gene="rpsC"
                     /locus_tag="PK242_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOZ94344.1"
                     /translation="MGQKIHPTGFRLSVSRNWASRWYASNRDFAGMLAEDIKVREYLK
                     AKLKNAAVSRVLIERPAKNARITIYSARPGVVIGRKGEDIEKLKKELADQLGVPVAVN
                     IEEVRKPEVDAQLIADSITQQLEKRIQFRRAMKRAMQNAMRLGAQGIKIMSSGRLNGI
                     EIARTEWYREGRVPLHTLRADIDYGFSEAHTTYGAIGVKVWVYKGDTLGRNDAPSLDS
                     TPRPEGEERRGPRGPRRDGRGGPGGDRRGGPRRDARGGGSGAPADGSDKPAEGQGAEA
                     KPAVKRVRKAAPAAAADGAQGE"
     gene            complement(4372..4710)
                     /gene="rplV"
                     /locus_tag="PK242_09760"
     CDS             complement(4372..4710)
                     /gene="rplV"
                     /locus_tag="PK242_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOZ94345.1"
                     /translation="MAATETRAVLRGVRLSVDKGRLVADLIRGKKVDQALNILNFTQK
                     KAAVIVKKVLESAIANAEHNDGADIDELRVKTIYVEQGMTLKRSAARAKGRGARLSKP
                     TCHVYVTVGN"
     gene            complement(4720..4995)
                     /gene="rpsS"
                     /locus_tag="PK242_09765"
     CDS             complement(4720..4995)
                     /gene="rpsS"
                     /locus_tag="PK242_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOZ94346.1"
                     /translation="MTRSLKKGPFVDHHLLAKVDKAVASKDKKPIKTWSRRSMMLPEF
                     IGLTIAVHNGKQHVPVYVTDQMIGHKLGEFALTRTFKGHAADKKAKK"
     gene            complement(5008..5832)
                     /gene="rplB"
                     /locus_tag="PK242_09770"
     CDS             complement(5008..5832)
                     /gene="rplB"
                     /locus_tag="PK242_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOZ94347.1"
                     /translation="MAVIKIKPTSPGRRGQVKISRDHLHKGEGYAPLLEPQFQKAGRN
                     NNGHITTRHKGGGHRHHYRVIDFRRNKDGIAAKVERIEYDPNRSAHIALVCYADGERR
                     YIIAPRGVEVGSQLMSGSEAPIRAGNTLPIRNIPVGSTIHCIELKPGAGAQIARSAGT
                     SATLLAREGTYAQVRMRSGEVRRVHIECRATIGEVANEEHSLRQFGKAGVKRWMGIRP
                     TVRGIVMNPVDHPMGGGEGRSKSGRHPVDPWGNLTKGYRTRSNKRTQNMIVSRRKK"
     gene            complement(5836..6171)
                     /gene="rplW"
                     /locus_tag="PK242_09775"
     CDS             complement(5836..6171)
                     /gene="rplW"
                     /locus_tag="PK242_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOZ94348.1"
                     /translation="MSRINPTPKERQFDEGRLMQVLVAPVISEKATMVAEKSNAVTFK
                     VLRSATKPEIKAAVELLFNVKVRGVSVANIKGKTKRFGRSIGRRDHVRKAYVLLQEGQ
                     ELNLSGEAA"
     gene            complement(6168..6788)
                     /gene="rplD"
                     /locus_tag="PK242_09780"
     CDS             complement(6168..6788)
                     /gene="rplD"
                     /locus_tag="PK242_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOZ94349.1"
                     /translation="MQLELLNDQGQAASKVDAPETLFGRDYNEALVHQLVVAYQANAR
                     QGTRAQKDRAQVKHSTKKPFRQKGTGNARAGMTSSPLWRGGGRIFPNSPFENFSQKLN
                     KKMYRAGMASILSQLVRDGRLAVVESIKLDSPKTKQLAQKFKDMKLDSVLVIADEVDE
                     NLYLASRNLGNVLVVEPRYADPLSLVHYKKVLVTKGAIDQLKEMFA"
     gene            complement(6788..>7251)
                     /gene="rplC"
                     /locus_tag="PK242_09785"
     CDS             complement(6788..>7251)
                     /gene="rplC"
                     /locus_tag="PK242_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOZ94350.1"
                     /translation="AKAGVEAGEITQEFRVDGNVAGKYAAGTALPVAEVFAAGQKVDV
                     QGTSIGKGYAGTIKRHNFSSQRASHGNSRSHNVPGSIGMAQDPGRIFPGKKMTGHMGD
                     ETVTTQNLDLIRIDEARQLLLIKGAVPGARGGFVTVRPAVKAKAAANEGAN"
BASE COUNT          962 a         2351 c         2509 g         1429 t
ORIGIN      
        1 cttcggcctg catcttgtcg cgcaggtcgg tgaacttggc ctgctccttc cagatttcgg
       61 gcttggcgcc ggtctggccc ttgtccgagc cctcgacgaa ggcgtggaac ggcagcttgg
      121 acgcgatggc gatgacgtcg ccgtcgatcg ccgcctgggc cgagttgaac ggcaccttgc
      181 cctgcaccat ggcacccagg ctgccgaaat ggtagcccag cagggtgaac gcgccgcggc
      241 ggtagtgcac ggcgtcttcg gccttggcgt actgctgctg cgcgaaggct ggcgccgcgg
      301 cgccggcgat ggccagcgtg aacgccagga cactgagttt cttcttcatc tgaaagactc
      361 cttgtgggac ggggtcggcg ccggcgcggc gccagccacc gagggacaaa gcgtgcccgt
      421 ggcaacaccg ggcgccggaa tcactacact gtgaccccgc aacgcaccgc accgccaggt
      481 ccgccgcgca agcatagccg gttgggcgcc aggccgtgcg gcgtgttcgc gcttgaccct
      541 gtatcgccct tcgctcgatc gtccgccatg cacaccgtcc gcatctggga tctgcccacc
      601 cgcctgtttc actgggttct gttcgtctgc gtcgtggcgc tggtggtcac cgccaaggtg
      661 ggcggcaacg ccatgaacct gcacctgcgg ctgggctacg tggtgttcac cttgctgctg
      721 tttcggctgc tgtggggctt cgtgggtggt cactggtcgc gctttgccag cttcttcccc
      781 acgccggggc ggctgacgcg ctacctgggc gggcgcgcca cgaccgccga caccgccggc
      841 cacaaccccc tgggcgcgct gtcggtgctg gccatgctgc tggtgctggg cgcgcaggtg
      901 ggctccggcc tgttcagcga cgacgagatt gccttttccg ggccgctcac ggccctggtg
      961 tccaacgcca cggtgggccg cctgaccgaa tggcacagcc actggggtca gtacctggtg
     1021 atcgggctgg tgctgctgca cgtgctggcg atcgtggtgt accggctgcg acgccagaac
     1081 ctggtggggc ccatggtggt gggcgacaag acgctgcccg agccggtgac cgcggcgcgc
     1141 gacagcgccg gcacgcgcct gctggcgctg gccctgctgg gcgtggcggc ggcgctggtg
     1201 tatgcggtgg tgcagctcgg cagccgcggt gccctggggt agactggaca cgaccgccgc
     1261 cgtgcccgcg cccatgctgc gcaccgaagt tcagtgacca cccgcccgcc gatccacctt
     1321 gcccccgccc gggagcccgc gcactacgac gcggcgcgcg ccctgtttcg tgagtacgcc
     1381 gatcagctcg gggtcgacct gtgctttcag ggcttcgagg ccgaactggc tggcctgcct
     1441 ggcgactacc aggaaccggg cggcacgttg ctgctggcct gggtcgacgg cgagctggcc
     1501 ggctgctgcg cgctgcgccc gatcgaggac gtggattacg ccaacgccgc cgaaatgaag
     1561 cggctgttcg tgcgcaaggc ctttcgcggc ttcggcctgg gccgccagct ggccgaggcg
     1621 gtgctggaag ccgcggtgcg cctgggctac gacagcgtgc tgctcgacac gctggacgac
     1681 atggaggcgg cgcgggcgct gtacgaagac ctgggcttcg tcgaaatccc gccctactac
     1741 cacaacccgc tgccgggggc gcactacctg aaggtcgagc tgcgcgtctg acggcgccgc
     1801 cgcgcccccg tggatggaca ataaataggc cgctggcgcg cgcccagctt gcgccagcag
     1861 ctactattta tgcagcttgc gaagccagac tcaggctgcg cgcagctgcg ccagcagggt
     1921 ggcgccgtcg ctcacctcga acttgcctgg cgcttcgacg ttgagtgccg ccaccttgcc
     1981 gtccttgacc agcatcgagt agcggttgct gcgcagcccc aggcccttgc cgtgcaggtc
     2041 cagcgtcagg ccggtggcct gggcgaacgc ggcgtcaccg tcggccagca tgcggatctg
     2101 gccggcggtc ttctgctcgc gcgcccaggc gcccatgacg aaggcgtcgt tgaccgacag
     2161 gcaccagatc tcgtccaccc cggccgcctt cagcgccgcg gcgtgctgca ggtagctggg
     2221 cacatgcttg gccgagcagg tgggcgtgaa ggcgcccggc acggcgaaca cggcgatggt
     2281 cttgccggcg gcagccttct gcacctcgac cgggttgggg ccgatgctgc agccgttgcc
     2341 ttcgacttcg atgtattccg acagagtgac ggcgggcagc gcgtctccga ccttgatcat
     2401 gcggggctcc ttgaaggtgt gtgggggtgc cgcggccgtg acgcggcgcg gcggtacaaa
     2461 cgaaaacagc ccacgcattg tgggctgttt cgggtcggcg cgctggtcgc gccgggcttt
     2521 gggctgagcc cgcggcgggt tttcagacct gagcggcctt ctgcaccagg cgggtggcga
     2581 cccagttctt ggtcttggac agcgggcggc tttcggtgat ctcgatggtg tcgcccagct
     2641 tgtactggcc gttttcgtcg tgcgcatggt acttggacga gcgcatcacg atcttgtcgt
     2701 agatcgggtg cttgacgcgg cgctcgacca gcaccgtcac cgtcttggcg cgcttgtcgc
     2761 tgacgacctt gccgatcagg gtgcgcttga gggatggttg ggattccgtc atggtcactc
     2821 cttacttggc ggcggcctgc ttttcgacga tcagcgtctt ggcgcgggcg atggcgcggc
     2881 gcgtggcctt gagctggctg gtgttgctca gctgctgcgt gcccttctgc atgcgcaggt
     2941 tgaaatgggc tttctgcagc tccttgacct cggcctgcag gccggcgacg tctttctgac
     3001 gcagttctgc ggttttcatc tgacgttcct cctgctcact ggccaatcat gcgggcgacg
     3061 aaggtcgtgc gcagcggcag tttggcggcg gccagggcaa acgcctcgcg cgccagctct
     3121 tccggcacgc cgacgatttc gtacagcacc ttgccgggct ggatctcggc cacgtagtac
     3181 tcggggttgc ccttgccgtt acccatacgg acttcggccg gcttggtgga gatcggcttg
     3241 tcggggaaca cgcggatcca gatgcggcca ccgcgcttga cgtggcgcga gatggcgcgg
     3301 cgcgcggatt cgatctggcg cgcggtcagg cgaccgcgat cggtggactt gaggccgaag
     3361 tcgccaaagg ccacggtatt gccacgggtg gcaatgccgg tgttgcggcc cttgtgctcc
     3421 ttgcggtact ttctgcgtgc gggttgcagc atggtttact ctccttgggc gccgtccgcg
     3481 gctgcagcgg gcgcggcttt gcggacgcgc ttaacggcgg gtttggcctc ggccccctgg
     3541 ccttcggcgg gtttgtcact gccatcggcg ggcgcaccgg agccgccgcc acgcgcgtcg
     3601 cggcgcgggc caccgcggcg atcgccgccc gggccaccgc ggccgtcacg gcgcgggcca
     3661 cgcggaccgc ggcgctcttc gccttcgggg cgcggcgtgc tgtccagcga gggggcatcg
     3721 ttgcggccca gggtgtcgcc cttgtagacc cagaccttga cgccgatggc gccgtaagtg
     3781 gtgtgggcct cggagaagcc gtagtcgatg tcggcgcgca gcgtgtgcag cggcacgcgg
     3841 ccttcgcggt accactcggt gcgggcgatc tcgatgccgt tcagacggcc cgaggacatg
     3901 atcttgatgc cctgggcgcc caggcgcatg gcgttctgca tggcgcgctt catggcgcgg
     3961 cggaactgga tgcgcttttc gagctgctgg gtgatggaat cggcgatcag ctgggcatcg
     4021 acttcgggct tgcgcacttc ttcgatgttc accgccaccg gcacgcccag ctggtcggcc
     4081 agttctttct tgagtttctc gatgtcctcg cccttgcggc cgatcaccac gcccggacgc
     4141 gccgagtaga tggtgatgcg ggcgttcttg gccggacgct cgatcagcac gcgcgagacg
     4201 gcggcgttct tgagcttggc cttcaggtac tcgcgcacct tgatgtcttc ggccagcatg
     4261 ccggcgaagt cacggttgct ggcgtaccag cggctggccc agttgcggct gaccgaaaga
     4321 cggaagccgg tcggatggat tttctgtccc atggtcttct cgtggccttc ctcagttgcc
     4381 caccgtcacg tacacatggc acgtgggctt ggacaggcgc gcaccgcggc ccttggcgcg
     4441 ggccgccgag cgcttgagcg tcatgccctg ctcgacgtag atggtcttga cacgcagttc
     4501 gtcgatgtcg gcaccgtcgt tgtgctcggc gttggcgatg gccgactcga gcaccttctt
     4561 gacgatgacg gcggccttct tctgcgtgaa gttcaggatg ttgagcgcct gatcgacttt
     4621 cttgccgcgg atcaggtcgg ccaccaggcg gcctttgtcc accgacaggc gaacgccgcg
     4681 gaggacggca cgtgtttccg ttgctgccat ggtgattcct tatttcttgg ctttcttgtc
     4741 cgccgcgtga cccttgaagg tccgcgtcag cgcaaattcg cccagcttgt ggccgatcat
     4801 ctggtcggtc acgtacaccg gtacgtgctg cttgccgttg tgcacggcga tcgtcaggcc
     4861 gatgaactcc ggcagcatca tggagcggcg cgaccaggtc ttgatgggct tcttgtcctt
     4921 gctggccacg gccttgtcga ccttggcgag caggtggtgg tccacaaacg gacccttttt
     4981 cagagaacga gtcatttgcg gaggtcctta cttcttgcgg cgcgacacga tcatgttctg
     5041 cgtgcgcttg ttgctgcggg tgcggtagcc cttggtcagg ttgccccatg ggtcaaccgg
     5101 atgtcggccg gatttgctgc gaccctcgcc gccgcccatc gggtggtcga ccgggttcat
     5161 cacgatgccg cgcacggtcg gacggatgcc catccagcgc ttgacaccgg ccttgccgaa
     5221 ctggcgcagg ctgtgctctt cgttggccac ttcaccgatg gtggcgcggc actcgatgtg
     5281 cacgcggcgc acttcgcccg aacgcatgcg cacctgggcg taggtgccct cgcgcgccag
     5341 cagcgtggcc gaggtgccgg ccgagcgcgc gatctgcgcg ccggcgcccg gcttgagctc
     5401 gatgcagtgg atggtcgaac ccaccgggat gttgcggatc ggcagcgtgt tgccggcgcg
     5461 gatcggcgct tcggagccgc tcatgagctg gctgcccacc tccacgccac gcggtgcgat
     5521 gatgtagcgg cgctcaccgt cggcatagca caccagggcg atgtgggccg agcggttggg
     5581 gtcgtactcg atgcgctcga ccttggcggc gatgccatcc ttgttgcgac ggaaatcgat
     5641 cacgcggtag tggtggcgat ggccgccgcc cttgtggcgc gtggtgatgt ggccgttgtt
     5701 gttgcggccg gccttctgga actggggctc cagcagcggg gcgtaaccct cgcccttgtg
     5761 caggtggtcg cgcgaaatct tgacctggcc acggcggccc ggcgacgtcg gtttgatctt
     5821 gatgacagcc atggcttacg cagcctcccc ggacaggttc agctcctggc cttcctggag
     5881 cagcacatag gccttgcgca cgtggtcacg gcgaccgatg ctgcggccaa agcgcttggt
     5941 cttgcccttg atgttggcca cggacacgcc acggaccttc acgttgaaca gcagctcgac
     6001 cgcggccttg atctcgggct tggtggcgct gcgcagcacc ttgaaggtga cggcgttgga
     6061 cttttcggcc accatcgtgg ccttctcgga aatcaccgga gcgacgagca cctgcatcag
     6121 gcggccttcg tcgaactggc gctctttcgg cgtcgggttg atgcggctca tgcgaacatc
     6181 tccttgagct ggtcgatggc gcccttggtg accagcacct tcttgtagtg caccagcgac
     6241 agcggatcgg cgtagcgcgg ctcgaccacc agcacgttgc cgaggttgcg cgacgccagg
     6301 tacaggttct cgtcgacctc gtcggcgatc accagcacgg agtcgagctt catgtccttg
     6361 aacttctgcg ccagctgctt ggtcttgggc gaatcgagct tgatcgactc gaccaccgcc
     6421 aggcggccat cgcgcaccag ttgcgacagg atggacgcca tgccggcgcg gtacatcttc
     6481 ttgttgagct tctggctgaa gttctcgaac gggctgttcg ggaagatgcg accgccccca
     6541 cgccacagcg gcgaggaggt catgccggcg cgcgcgttgc cggtgccttt ctggcggaac
     6601 ggcttcttgg tgctgtgctt gacctgggcg cggtctttct gcgcccgcgt gccctggcgg
     6661 gcgttggcct ggtaggccac caccagctgg tgcaccagcg cttcgttgta gtcacgaccg
     6721 aacagggttt cgggcgcatc caccttggac gccgcctgac cttggtcgtt caggagttcg
     6781 agctgcatca gttggccccc tcgttggcag cggccttggc cttgacagcc gggcggacgg
     6841 tgacgaaacc gccacgcgca ccgggcacgg cgcccttgat gagcagcagc tgacgggctt
     6901 cgtcgatgcg gatcaggtcg aggttctggg tggtgacggt ctcgtcgccc atgtgaccgg
     6961 tcatcttctt gcccgggaag atgcgacccg ggtcctgcgc catgccgatg gagcccggca
     7021 cgttgtgcga acggctgttg ccgtgcgagg cgcgctgcga gctgaagttg tggcgcttga
     7081 tggtgccggc gtagcccttg ccgatggaag tgccctgcac gtccaccttc tggccggcgg
     7141 caaacacctc ggccacgggc agcgcggtgc cggcggcgta cttgccggcc acattgccgt
     7201 cgacacggaa ttcctgggtg atttcaccgg cttcgacgcc cgccttggcg a
//
LOCUS       DAOOOG010000130         7251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_12181_length_7251_cov_20.710812, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000130 DAOOOG010000000
VERSION     DAOOOG010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12181_length_7251_cov_20.710812"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4093)
                     /locus_tag="PK242_09790"
     CDS             complement(<1..4093)
                     /locus_tag="PK242_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOZ94351.1"
                     /translation="MTDGLIRIRGARQHNLKNLDLDLRTGELTVVTGVSGSGKSSLVF
                     DTLYAEGQRRYVETFSPYARQFLDRMDKPAVDQVEGVPPAIAIDQTNPVRSSRSTVGT
                     MTELNDHLKLLFARAGQLFDRATALPVRHDNPETIYAELQRRAARFTAGPPPGEAAPS
                     GGSEARAAGSVGAMNDPRLVITFAVELPASTSADEVQQWLSTAGFTRVQAERVEGDKK
                     VLDVVADRFRVGSAEKARVIEAIELALLRGGGRMSVYALPEQGEPELWRFSTGLHCPE
                     SDVRYGDPIPSMFSFNSAVGACPACRGFGRVIGVDWGLVIPDEKLTLRAGAIKPIQTP
                     AWKECQDDLMRHAEAAGIPRDTPWNKLTPEQRHWVIEGTPTYKEGNWNRQWYGVRRFF
                     AYLETKAYKMHIRVLLSKYRSYTACPSCGGARLKTEALLWRVGSKQDADAVIAPSQRF
                     MPQGVAWSRAQLEQLPGLCLHDLMLLPIERLARFFDRLQTPSPAGGGLGWGPAAPAST
                     DAGPSPQPSPQRGEGATAAASTLTPSPAGGRLGWGPAAPASTDAGPSPQPSPQRGEGA
                     TAADSTLTPSPAGGGLGWGPAAPVASADAQALHLILEEIQTRLKYLVDVGIGYLTLDR
                     QSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMDRITEAMQRLKRAGNT
                     LVVVEHDPAVMLAADRVLDFGPGPGAAGGRIVFDGTPDALRGADTLTGAYLGGRKSIG
                     LGFARLVKPDTPRLLLEGVREHNLRGIDVELPLQRLVTITGVSGSGKSTLIQDVLAPA
                     LLRHFGQATEAPGAHDRLLGAEQLSGVVFVDQSPIGKTARSNPVSYVGAWDAIRKLFA
                     DAPLARQRGYTAAKFSFNSGDGRCPTCGGSGFEHVEMQFLSDVYLRCPDCNGQRYRPE
                     VLEVRIDRQAPSDLRARHLNVADVLELTVAEALQLFARDREVVRVLQPIVDVGLDYVR
                     LGQPVPTLSGGEAQRLKLAGHLAEAAQTTSASRQPMARKGQLFLFDEPTTGLHFDDIA
                     KLMRALRKLLDAGHSLVVIEHNLDVMRASDWLIDLGPEGGDGGGRIVAQGTPDEVMNH
                     STSHTGAALRAYAQALGDGAHEARELLPPLGEGWDGGRAAQHQTLGIGHAAGPHPNPP
                     PAGEGARSAIDIVRAREHNLKNLSVAIPRGRFNVVTGVSGSGKSTLAFDIVFNEGQRR
                     YLESLNAYARSIVQPAGRAAVDAVYGIPPTVAIEQRLSRGGRKSTVGTVTEVWHFLRL
                     LYVKLGVQHCIHDGARVEPQTPERIAAQILTRFRGQTIGLLAPLVVARKGVYTELADW
                     ARPRGFTHLRVDGEFLPTTGFPRIDRFKEHTIELPVMSLPVSPG"
     gene            complement(4229..4378)
                     /locus_tag="PK242_09795"
     CDS             complement(4229..4378)
                     /locus_tag="PK242_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94352.1"
                     /translation="MWKIALGFVLFAALSLYVIMQAGDKVDMSGEKHGAEAVHVPEAP
                     ASAAK"
     gene            complement(4518..6185)
                     /locus_tag="PK242_09800"
     CDS             complement(4518..6185)
                     /locus_tag="PK242_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ94353.1"
                     /translation="MATATPAKAIRPMTPEERKVIFASSLGTVFEWYDFYLYGSLAAI
                     IAKQFFAGLDPTSAFIFALLAFAAGFIIRPFGALVFGRLGDMIGRKYTFLVTILIMGI
                     STFIVGILPNYASIGVAAPVILICLRLLQGLALGGEYGGAATYVAEHAPHGRRGAYTS
                     WIQTTATLGLFLSLLVILGTRTIMGEPAFNDWGWRIPFLVSILLLAVSVWIRLSLSES
                     PAFQKMKAEGKTSKAPLSESFGQWKNLKIVLLALFGLTAGQAVVWYTGQFYALFFLTQ
                     QLKVDAVTANLMIAAALVIGTPFFVVFGSLSDKIGRKPIIMAGCLLAILTYFPVFKAL
                     TEAANPDLAAAQAKNKVVVVADPAECSFQFNPTGTVKFTSSCDIAKQVLAAGSVSYEN
                     EIAPAGTPAVIKIGSHVIESYTSKGIPADEAKAKDAAFKKAVADTLKADGYPTKADPA
                     KMNKGMMVAILVYLVILVTMVYGPIAAMLVELFPTRIRYTSMSLPYHIGNGWFGGLLP
                     TTSFAIVAQTGNMYNGLWYPIIVAAMTLVIGTLFIRETKDVDIYAED"
     gene            6679..6987
                     /locus_tag="PK242_09805"
     CDS             6679..6987
                     /locus_tag="PK242_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HOZ94354.1"
                     /translation="MSDPMVERVQRNPKYQQLKQARSRFGWLLTILMLVVYYGYIGLI
                     AFDKEFLARPLGSGVTTLGVPIGMAVIVFTIAIVGIYVRRANREYDALTRDILEDATQ
                     "
     gene            6984..>7251
                     /gene="actP"
                     /locus_tag="PK242_09810"
     CDS             6984..>7251
                     /gene="actP"
                     /locus_tag="PK242_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667773.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOZ94355.1"
                     /translation="MSTAKRIGLALATLTLAGSALAAGGDVGQAAKQSTNWIAIIMFT
                     VFVAATLWITKWAAGRTKSAADFYTAGGGITGFQNGLAIAGDYMS"
BASE COUNT         1156 a         2373 c         2571 g         1151 t
ORIGIN      
        1 tgccggggct gacgggcagg ctcatcaccg gcagctcgat ggtgtgttcc ttgaagcggt
       61 cgatgcgcgg aaagccggtg gtcggcagga actcgccatc cacgcgcagg tgggtaaagc
      121 cacgcgggcg cgcccagtcg gccagctcgg tgtagacgcc cttgcgcgcc accaccagcg
      181 gcgccaaaag gccgatggtc tggccgcgaa agcgcgtgag gatctgcgcc gcgatgcgct
      241 cgggcgtctg cggctcgacg cgcgcgccgt cgtggatgca gtgctgcacg cccagcttga
      301 cgtagagcag gcgcagaaag tgccagacct cggtgacggt gccgaccgtg ctcttgcgcc
      361 cgccgcgcga caggcgctgc tcgatcgcca ccgtgggcgg gatgccgtac acggcgtcga
      421 ccgcggcgcg tccggccggc tgcacgatgg agcgtgcata ggcgttgagc gattccagat
      481 agcggcgctg gccctcgttg aacacgatgt cgaaggccag cgtggatttg cccgagccgg
      541 agacgccggt gacgacgttg aagcggccgc gcgggatggc gacactgagg ttcttcaggt
      601 tgtgctcgcg cgcgcgcacg atgtcgatgg cgctcctggc tccctccccc gctgggggag
      661 ggttggggtg ggggccagcg gcgtgtccga tccccaacgt ctggtgttgt gcggcgcggc
      721 ccccatccca gccttccccc agcgggggaa ggagttctcg cgcttcgtgg gcgccgtcgc
      781 ccagggcctg cgcgtacgcg cgcagcgccg cgccggtgtg cgaggtcgag tggttcatca
      841 cctcatccgg cgtgccctgc gccacgatgc gcccaccgcc gtcgccgccc tcggggccca
      901 ggtcgatcag ccagtcgctg gcgcgcatca cgtccaggtt gtgctcgatc accaccagcg
      961 agtggccggc gtccagcagc ttgcgcagcg cccgcatcag cttggcgatg tcgtcgaagt
     1021 gcaggccggt ggtgggctcg tcaaacagaa acagctggcc cttgcgcgcc atcggctggc
     1081 ggctggccga cgtggtctgc gccgcttcgg ccagatggcc cgcgagcttg agccgctgcg
     1141 cctcgccgcc cgacagcgtg ggcaccggct ggcccaggcg cacgtagtcg aggccgacgt
     1201 cgacgatggg ctgcagcacg cgcaccacct cgcggtcgcg ggcaaacagc tgcagcgcct
     1261 cggccaccgt caactccagc acgtcggcca cgttgaggtg gcgcgcacgc aggtcgctgg
     1321 gcgcctggcg gtcgatgcgc acctccagca cctcggggcg gtagcgctgg ccgttgcagt
     1381 cggggcagcg caggtagacg tcggacagga actgcatctc gacgtgctcg aagcccgagc
     1441 cgccgcaggt cgggcagcgc ccgtcgcccg agttgaagct gaacttggcc gcggtgtagc
     1501 cgcgctggcg cgccagcggg gcgtcggcaa acagcttgcg gatggcgtcc caggcgccga
     1561 cgtagctgac cgggttggag cgcgcggtct tgccgatggg tgactgatcg acgaacacca
     1621 cgccggaaag ctgctcggca cccagcaggc ggtcatgcgc acccggtgcc tcggtggcct
     1681 ggccgaagtg gcgcagcagc gccggcgcca gcacgtcctg gatcagcgtg gatttgcccg
     1741 agcccgacac gccggtgatg gtgaccaggc gctgcagcgg cagctcgacg tcgatgccgc
     1801 gcaggttgtg ctcgcgcacg ccttccagca gcaggcgcgg cgtgtcgggc ttgaccaggc
     1861 gcgcaaaacc caggccgatg gacttgcgcc cgcccagata ggcgccggtc agggtgtcgg
     1921 cgccgcgcag cgcatcgggc gtgccgtcga acacgatgcg cccgcccgct gcgccggggc
     1981 cgggcccgaa gtcgagcacg cggtcggccg ccagcatcac ggcggggtcg tgctcgacca
     2041 ccaccagcgt gttgccggcg cgcttgaggc gctgcatggc ttcggtgatg cggtccatgt
     2101 cgcgcgggtg caggccgatg ctgggctcgt ccagcacgaa cagcgtgttg acgagggacg
     2161 tgcccagcgc cgtggtcagg ttgatgcgct gcacctcgcc gccgctcagg gtgcggctct
     2221 ggcggtcgag cgtgaggtag ccgatgccca cgtcgaccag gtacttcagg cgggtctgga
     2281 tttcttcgag gatcaggtgc agcgcttgcg cgtcggcgct ggcgacgggc gcggcgggcc
     2341 cccaccccaa ccctccccca gcgggggagg gagtgagggt ggagtcggcg gctgttgctc
     2401 cctcgcccct ttggggagag ggctggggtg aggggccggc gtcggttgat gcgggtgcgg
     2461 cgggccccca ccccagcctt cccccagcgg gggagggagt gagggtggag gcggcggctg
     2521 ttgctccctc gcccctctgg ggagagggct ggggtgaggg gccggcgtcg gttgatgcgg
     2581 gtgcggcggg cccccacccc agccctcccc cagcggggga aggggtttgc agtcggtcga
     2641 aaaagcgcgc cagccgctcg atcggcagca gcatcaaatc gtgcaggcac aggccgggga
     2701 gctgttccag ttgcgcccgg ctccacgcca cgccctgcgg catgaagcgc tgcgacggtg
     2761 cgatcacggc gtccgcgtcc tgcttgctgc ccacccgcca cagcagcgcc tcggtcttca
     2821 ggcgcgcgcc gccgcagctg gggcaggcgg tgtagctgcg gtacttggac aagagcacgc
     2881 ggatgtgcat cttgtaggcc ttggtctcca gataggcgaa gaagcgccgc acgccgtacc
     2941 actgcctgtt ccagttgcct tccttgtacg tgggcgtgcc ctcgatcacc cagtgccgtt
     3001 gctcgggcgt gagcttgttc cagggcgtgt cgcgcgggat gccggcggcc tcggcgtgac
     3061 gcatcaggtc gtcctggcac tccttccagg ccggggtctg gatcggcttg atggcaccgg
     3121 cgcgcagcgt gagcttttcg tccggaatca ccagtcccca gtccaccccg atcacgcggc
     3181 caaagccgcg gcaggccggg caggcgccca ccgccgagtt gaagctgaac atcgacggga
     3241 tcggatcgcc gtagcgcaca tcgctttcgg ggcagtgcag gccggtggaa aacctccaca
     3301 gttcgggctc gccctgttcg ggcagggcat acacgctcat gcgcccgccg ccacgcagca
     3361 gcgccagctc gatggcctcg atgacgcgcg ccttctcggc gctgccgacc ctgaatcgat
     3421 ccgccaccac atcaaggacc ttcttgtccc cttccacgcg ctcggcctgc acgcgggtga
     3481 agccggcggt cgacagccat tgctgcacct cgtcggcgct ggtgctggcc ggcagttcca
     3541 ccgcaaaggt gatgaccaga cgcgggtcgt tcatggcccc cacgctcccc gctgcgcggg
     3601 cttcgctgcc ccccgagggg gctgcttcac ctggaggcgg cccggcggtg aagcgcgcgg
     3661 cgcgccgctg cagctcggcg taaatggttt ccgggttgtc gtgccgcacg ggcagcgcgg
     3721 tggcgcggtc gaacagctgg ccggcgcggg cgaacaggag cttcaggtga tcgttcagct
     3781 ccgtcatcgt ccccaccgtg gagcgcgagc tgcgcaccgg gttggtctgg tcgatggcga
     3841 tcgccggcgg cacgccctcc acctgatcga ccgcgggctt gtccatgcgg tccagaaact
     3901 ggcgcgcata ggggctgaag gtctcgacgt aacggcgctg accctcggcg tacagggtgt
     3961 cgaacaccag gctggacttg cccgaaccgg acacgccggt gaccaccgtc agttccccgg
     4021 tgcgcaggtc caggtcaagg ttcttcaggt tgtgctgccg cgcgccgcgg atgcggatca
     4081 ggccatcggt cattgcgggt ccgtcagggg aagggccgca agattctagg ggccgcgggt
     4141 tggcactgcc ggccggcggg gcttgtggtc cggcccgtgc gcggccacag atcgggccgc
     4201 cggccgttgg ggattcaggc tcccggcgtc acttggcggc cgaagctggc gcttcgggca
     4261 cgtgcacggc ttcggcgccg tgcttttcgc cgctcatgtc gaccttgtca ccggcttgca
     4321 tgatgacgta gagcgaaagg gcagcaaaca acacaaaacc cagggcgatt ttccacatgt
     4381 tgaactcctg aatgacgaat cggggggctg aaaaccaact caccccagtc gcccggctga
     4441 tccaggcact ggggtgagcg gggtaaggct caggccatgc gcgccccggt cagcacgacc
     4501 ggaccgcgcc ggcaggctca atcctcggcg tagatgtcca cgtccttggt ttcgcggatg
     4561 aacagcgtgc caatcaccag cgtcatggcg gccacgatga tgggatacca caggccgttg
     4621 tacatgttgc cggtctgcgc cacgatggcg aacgaggtgg tgggcagcaa accgccgaac
     4681 cagccgttgc cgatgtggta gggcaggctc atggaggtgt agcggatgcg ggtcgggaac
     4741 agctcgacca gcatggcggc gatggggcca tacaccatgg tgaccagaat caccaggtac
     4801 accaggatgg cgaccatcat gcccttgttc atcttggcgg ggtcggcctt ggtggggtag
     4861 ccgtccgcct tcagggtgtc ggccacggcc ttcttgaagg cagcgtcctt ggccttggcc
     4921 tcgtcggcgg ggatgccctt gctggtgtag ctctcgatca cgtggctgcc gatcttgatg
     4981 acggcgggcg tgccggcagg cgcgatttcg ttctcgtagc tcaccgaacc ggcggccagc
     5041 acctgcttgg cgatatcgca ggagctggtg aacttgaccg tgcccgtggg gttgaactgg
     5101 aacgagcact cggccgggtc ggccaccacc accaccttgt tcttggcctg ggcagcggcc
     5161 aggtcagggt tggcggcttc ggtcagcgcc ttgaacaccg ggaagtaggt caggatggcc
     5221 agcaggcagc ccgccatgat gatgggcttg cgaccaatct tgtcggacag ggagccgaac
     5281 acgacgaaga acggcgtgcc gatcaccagc gcggcggcga tcatcaggtt ggcggtgacg
     5341 gcgtcgacct tgagctgctg cgtgaggaaa aacagggcgt agaactggcc cgtgtaccac
     5401 accacggcct ggccggcggt caggccgaac agggcgagca gcacgatctt gaggttcttc
     5461 cactggccga aggactcgga cagcggcgcc ttggaggtct taccctcggc cttcatcttc
     5521 tggaaggcgg gcgattcgga cagcgacagg cggatccaca ccgacaccgc cagcagcagg
     5581 atggacacca ggaacggaat gcgccagccc cagtcgttga aggccggctc acccatgatg
     5641 gtgcgcgtgc cgagaatcac cagcagcgac aggaacaggc ccagcgtggc ggtggtctgg
     5701 atccaggagg tgtaggcgcc acggcggccg tggggtgcgt gctcggccac gtaggtggcg
     5761 gcgccgccgt actcaccgcc cagcgccagg ccctgcagca ggcgcaggca gatcaggatg
     5821 accggagcgg ccacgccgat gctggcgtag ttgggcagga tacccacgat gaacgtggaa
     5881 atgcccatga tcaggatggt caccaggaag gtgtacttgc ggccaatcat gtcgcccagg
     5941 cggccgaaca ccagcgcgcc gaaggggcgg atgatgaagc cggcggcaaa cgccagcagg
     6001 gcgaaaatga aggccgaggt ggggtcgagg ccggcaaaga actgcttggc gatgatggcc
     6061 gccagcgaac cgtagaggta gaagtcgtac cactcgaaaa cggtgcccag ggaggaggcg
     6121 aagatcacct tcctttcctc gggcgtcatc ggccggatgg ccttggccgg ggttgcagtc
     6181 gccatgtgtc tgtctcctga gatgggggtg cgaacgagct gtgggacacg caacgatctg
     6241 tgtcctgcgg cgaaatgtcg gggcggtgcc tgaccgtttt ctgacgatga ccgacaggcg
     6301 gtgggcgcga aactgaagcc agactgacaa accccgtgac aaccctgagg ccacgttttt
     6361 cgatgcgaaa tcggaccccc gcagaaggcc atttcagccg ctgccgggct ggtgaaaacc
     6421 ccgacaaaaa aatctcgcca tctcgctgca cccgcctcgc ggtcgcgctg cgcgcacacc
     6481 cggcggttgc atttcagcgc cgccgcgaac ccggcatgaa tgaggcccgt cagacgatgg
     6541 caagcggtcg acggccagct tgagtttgca accgcatgtg acgtttcccg ccacgtaagt
     6601 ggttggtcag tgcctctgtc cagaatcgcg ccgcgcccgc cgatccggcc ggcgcacacc
     6661 ctggaaggag accgagacat gagcgacccg atggtcgagc gcgtccagcg caaccccaag
     6721 taccagcagc tcaagcaggc gcgcagccgc ttcggctggc tgctcacgat cctgatgctg
     6781 gtcgtgtatt acggctacat cggcctgatc gcgttcgaca aggagttcct ggcccgtccg
     6841 ctgggcagcg gcgtcaccac ccttggcgtg ccgatcggca tggcggtgat cgtgttcacg
     6901 atcgccatcg tcggcatcta tgtgcgccgc gccaaccgcg agtacgacgc cctgacgcgc
     6961 gacatcctgg aggacgccac ccaatgagca ccgcaaaacg catcgggctt gcgctggcca
     7021 ccctgacgct ggctggcagc gcgctggccg ctggcggcga cgtcggccag gccgccaagc
     7081 agtccaccaa ctggatcgcc atcatcatgt tcacggtgtt cgtggcggcc acgctgtgga
     7141 tcaccaaatg ggcggccggg cgcaccaagt cggcggccga cttctacacc gccggtggcg
     7201 gcatcaccgg cttccagaac ggcctggcga tcgccggcga ctacatgtcg g
//
LOCUS       DAOOOG010000131         7211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_12283_length_7211_cov_25.034237, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000131 DAOOOG010000000
VERSION     DAOOOG010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12283_length_7211_cov_25.034237"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PK242_09815"
     CDS             <1..548
                     /locus_tag="PK242_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOZ94356.1"
                     /translation="EKEARGGKKTLLLGPNCPGVITPDEIKIGIMPGHIHKKGRVGVV
                     SRSGTLTYEAVAQLTELGIGQSSAVGIGGDPINGIKHIDVMKAFNDDPDTDAVIMIGE
                     IGGPDEAEAARWCKDHMKKPIVGFIAGVTAPPGKRMGHAGALISGGADTADAKLAIME
                     ECGFKITRNPSEMGRLLKALL"
     gene            653..1360
                     /locus_tag="PK242_09820"
     CDS             653..1360
                     /locus_tag="PK242_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOZ94357.1"
                     /translation="MEDFLTAHFWLAVGQIIMIDILLGGDNAVVIALACRGLPPHQRT
                     KGIIWGTVGAIVLRVILIAFALALLQIPFLKLVGALLLIWIGVKLIAPEEEDGHDGIQ
                     SSDKLWGAVKTVIVADLVMSVDNVIAIAGAAQGAGEQHQLALVIFGLLVSIPIIVWGS
                     QLVLKLMDRFPVIILLGGMLLGWIAGTMAQSDPALATFIPQDRAWHYGMGVVGALLVL
                     ALGKFLERRRASASAVT"
     gene            1428..2213
                     /locus_tag="PK242_09825"
     CDS             1428..2213
                     /locus_tag="PK242_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOZ94358.1"
                     /translation="MEFFYSALTDVGRVRANNEDAVRVDAEHGIVVLADGMGGYNAGE
                     VASAMAVDLIVEELGRWLHEAAAAATLRDVRRAMEICVDNANRAIFEAANTNDDCAGM
                     GTTLVMGAVLGSTLLVGHVGDSRAYRWRAGVLTLLTRDHSLLQEQIDIGLITPADAAA
                     SGYRNLVTRALGVEDTVLLDVQEVGIASGDVLLLCSDGLNDMLHDADIAAVLGSAESP
                     HEMSQHLIELANGRGGRDNVSVIVVKAQEPPRKPGMMAKLLRK"
     gene            2298..2960
                     /locus_tag="PK242_09830"
     CDS             2298..2960
                     /locus_tag="PK242_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOZ94359.1"
                     /translation="MPKLIVSIGGVVVKEVQLTKDRTTIGRRPYNDVVIDNLAVSGEH
                     AVLQMGAGQVTIEDLGSTNGTYVNGRSVKSEVLESEDSIEIGKYKIRFLDGEASAPSP
                     EPVGIHSDSATAIGLSLPMGIDAPPAPSAEQLVGPRVRVLSGLAAGRELPLTKVVTTI
                     GKPGICVASITRKSHGFDLAQVEGQQMARVNGVTVDVGPITLVDRDQMELGGVRLEFL
                     ER"
     gene            3086..5290
                     /locus_tag="PK242_09835"
     CDS             3086..5290
                     /locus_tag="PK242_09835"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOZ94360.1"
                     /translation="MLASALLVLLASLHAVGVWRAPLLDRLDEVLYDLRLRLTMPGTL
                     DERVVIIDVDEPSLARHGQWPWRRAEVARLIEELIERQQVAALGLDVVFAELDAGGGL
                     GALERLARGELRDNPAFLDWLARTAPTLDGDRLLAARLARAPVALGYYFSSDRAGSRT
                     GALPEPWIRPDRLPRGLLHWDGYGGNIAVLNGAARTAGFFNAVADPDGSVRAVPLVAG
                     FEGALYPSLSLATLRLGLGARQLREERLGPEADAPLQALWLGDDVHRWRLPVDDRGTV
                     LVPYRGPGGPQGGSFRYISASDVLQGRLAPAELKGRYALLGFTAPGLMDLRATPVAEV
                     YPGVEIHANLISGMLDGRVAVRPDYAAGYEVGLLVLLGGVLALGLPLLSVGGALGLGA
                     ALLAALLALDLSLYVGAGLALPLATPLALILIALTLDMALGYFVEGRAKRALARQFAT
                     YVPPELVQQMLQQPERYGMAARSQELTVMFCDLHGFTSLAETMEPQTLQTLLGEVLSH
                     LTEVIQTHQGTIDKYIGDCVMAFWGAPVAMPDHARRAVDAAVAIVASLRDLNADHAAR
                     GLPAVSVGIGLNTGVMSVGNMGSDLRRAYTVIGDAVNLAARLEALSRVYEVDLVASQA
                     TRDAAAAAGHVWQELDRVRVKGRHQAVSIHTVRAAAGQDDVALQEELAAWRQALSLWR
                     AGHFAEFGSAVEALQARNANYCLYRLYAERLVFLLRFPPGPDWDGTTVFDAK"
     gene            5311..6114
                     /locus_tag="PK242_09840"
     CDS             5311..6114
                     /locus_tag="PK242_09840"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HOZ94361.1"
                     /translation="MRLRVLGCSGAIARDCRTTSFLVDQDILIDAGTGVGELTRDEML
                     RIEHVFLTHSHLDHVACLPLMVDTMACDRIGRPIHVHALPQTIAALRAHVLNDVIWPD
                     FSRLPTPELPLIQFHPLQVGHTRELGGRVIEALPARHAVPALGFALRRQATDAPWWVF
                     SGDTSGHPDFWQRLNQLRVHSLVIETAFANRERELAQMAQHLSPETLAEELDRIDRRH
                     RFPIYITHTKPADTETIMREIAQFDQVRPPRREVAHDIRWLSAGHVFEL"
     gene            complement(6130..>7211)
                     /locus_tag="PK242_09845"
     CDS             complement(6130..>7211)
                     /locus_tag="PK242_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOZ94362.1"
                     /translation="RVRQLHAGKKQAPLWPIERARANKARIDWGNYTPPVPKFIGRRV
                     FQHIELAGLLPFIDWTPFFQTWDLAGKYPAILNDEVVGQEARRVFQDAQTMLKKIVDG
                     RWLTANAVVGFWPANSVHDDDIELYADEARQHVVLTWHGLRQQSEKQEDAAGQLRPSR
                     CLADFVVPRNAIDSVADGACQSGAGGQNAFKDYLGAFAVTAGIGVEDKVAEFMAAHDD
                     YSAITLKALADRLAEACAEWLHHRVRTDLWGYAADEGLSNEELIAERYRGIRPAPGYP
                     ACPDHSVKRPLFELLRTQEIGMGLTESYAMTPPASVSGFYFSHPQSSYFNVGRIDMTQ
                     VADMAARRGMAPAALERLLAPNLGL"
BASE COUNT         1127 a         2344 c         2509 g         1231 t
ORIGIN      
        1 aggaaaaaga ggccaggggc ggcaagaaga ccctgctgct gggccccaac tgccccggcg
       61 tcatcacgcc cgacgagatc aagatcggca tcatgccggg ccacatccac aagaagggcc
      121 gcgtcggcgt ggtgtcgcgc tccggcaccc tgacctacga agccgtggcg cagctgaccg
      181 agctgggcat cggccagtcg agcgcggtgg gcattggcgg tgacccgatc aacggcatca
      241 agcacatcga cgtcatgaag gccttcaacg acgaccccga caccgacgcc gtcatcatga
      301 tcggcgagat cggcggcccc gacgaggccg aagccgcgcg ctggtgcaaa gaccacatga
      361 aaaaaccgat cgtcggcttc attgccggcg tcacggcccc tccgggcaag cgcatgggcc
      421 acgcgggcgc gctgatctcc ggcggcgccg acacggccga cgccaagctg gcgatcatgg
      481 aagagtgcgg tttcaagatc acgcgcaacc cttcggaaat ggggcggctg ctcaaggcgc
      541 tgctgtgacc acgttgcgct gacgcgcaac cgcccgccag ccggacgcca tacgggcggt
      601 ccggctgtat catttcggaa gccgctcgca gccccgacaa gaaagacgcc ccgtggaaga
      661 cttcctgacc gcccatttct ggctggctgt cggccagatc atcatgatcg acatcctgct
      721 ggggggcgac aacgcggtcg tcatcgcgct ggcctgccgt ggcctgccac cgcaccagcg
      781 caccaagggc atcatctggg ggacggtcgg cgccatcgtg ctgcgcgtca tcctgatcgc
      841 gttcgcgctg gcgctgctgc agatcccgtt cctcaagctg gtgggcgcgc tgctgctgat
      901 ctggatcggg gtcaagttga tcgctcccga ggaggaggac ggtcacgacg gcattcagag
      961 cagcgacaag ctgtggggcg ccgtcaagac ggtcatcgtg gccgacctgg tgatgagcgt
     1021 cgacaacgtg atcgccatcg ccggtgccgc gcagggtgcg ggcgaacagc accagctggc
     1081 gctggtcatc ttcggcctgc tggtgagcat tcccatcatc gtctggggca gccagctggt
     1141 gctcaaactg atggaccgct tccccgtcat catcctgctg ggcggcatgc tgctgggctg
     1201 gatcgccggc accatggcgc agagcgaccc ggccctggca acgttcatcc cgcaggaccg
     1261 ggcgtggcac tacggcatgg gcgtggtggg cgcgctgctg gtgctggcgc tgggcaagtt
     1321 cctcgagcgt cggcgcgctt cggcttcggc cgtgacgtga gcgggcggcc tcacacgctg
     1381 caacgcgcat gcttcgtccc cgatccgcca ggccaccggt cacggagatg gagtttttct
     1441 acagcgccct gaccgacgtt ggccgcgtgc gcgccaacaa cgaagacgcg gtgcgcgtcg
     1501 acgccgagca tggcatcgtc gtgctggccg atggcatggg gggctacaac gccggcgagg
     1561 tggccagcgc catggcggtc gacctcatcg tcgaggaact gggtcgctgg ttgcacgagg
     1621 cggcggccgc ggccaccttg cgcgacgtgc gccgagccat ggaaatctgc gtcgacaacg
     1681 ccaaccgggc gatcttcgag gccgccaaca ccaacgacga ctgcgctggc atgggtacca
     1741 ccctggtgat gggggctgtg ctgggcagta ccctgttggt cggccatgtg ggagattcgc
     1801 gggcctatcg ctggcgtgcc ggtgtgctga cgctgctcac gcgcgatcat tccctgttgc
     1861 aggagcagat cgacatcggc ctgatcacgc cggcagatgc cgcagcttcg ggttaccgca
     1921 acctggtgac gcgcgccctc ggggtcgagg acacggtgct gctggacgtg caggaggttg
     1981 ggattgcttc cggggacgtg ctgctgcttt gctcggacgg cttgaacgac atgttgcacg
     2041 acgccgacat cgctgcggtg ctcggtagcg cagagtcgcc gcacgagatg agccagcacc
     2101 tgatcgaact ggccaacggg cgtggcggtc gtgacaatgt ctcggtgatc gtggtcaagg
     2161 cgcaggaacc gcccagaaag cccggaatga tggcgaaatt gctgaggaaa taggaaacag
     2221 ttggcttgcc gcgggtcgat ccctgaggtc caattgctcg tcggccatcg gttcgggcca
     2281 aaaaacaggg agtcttcatg ccgaagttga tcgtttcgat cggcggggtg gtggtcaagg
     2341 aggtccaact caccaaggat cgcaccacca ttggtcgtcg accttacaat gacgtggtca
     2401 ttgacaacct tgccgtcagc ggggaacacg ccgtcctcca gatgggcgct ggccaggtca
     2461 ccatcgagga tctgggcagc accaatggta cctatgtcaa cggcagatcg gtcaagagtg
     2521 aggtgctgga gagcgaggac agcatcgaga tcggcaagta caaaatccgg ttcctggatg
     2581 gtgaagcgag cgcgccgtct ccggagccag tcggcatcca cagcgacagc gctaccgcca
     2641 tcggtctgtc gctgcccatg ggcatcgatg cgcctcctgc gcccagcgcc gagcaactgg
     2701 tcgggccgcg cgtgcgtgtg ctcagtggcc tggcggccgg acgtgaactg ccgctcacca
     2761 aggttgtcac taccatcggc aagccgggta tctgcgtggc gtccatcact cgcaagtcac
     2821 atgggttcga cctggctcag gtggaagggc aacagatggc cagggtcaat ggcgtcaccg
     2881 tcgatgtggg ccctatcaca ctggtggaca gagaccagat ggagttgggc ggagttcggc
     2941 tggaattcct cgagcgctga acaggcgcat gccggatcgc agtggtcatc caccgcggag
     3001 gccaaacggc catgccgcgc gccttcctga cggcgcatgc ccctgaatca tggcttggca
     3061 agggtttcag cgacatcgcc ggcgcatgct ggccagcgcg ctgctggtgc tgctggcctc
     3121 gttgcacgca gtgggggtgt ggcgcgcgcc cctgctggat cggctcgacg aggtgttgta
     3181 cgacctgcgt ctgcggctga ccatgccggg cacgctcgac gagcgcgtgg tcatcatcga
     3241 cgtcgacgag cccagtctgg cgcgccatgg tcagtggccc tggcgacggg ccgaggtcgc
     3301 tcggttgatc gaggaactga tcgagcgtca gcaggtggct gcgctggggt tggacgtggt
     3361 gtttgccgag ctcgatgcgg gtggtggact gggtgccttg gagcggttgg cgcgcgggga
     3421 actgcgcgac aacccggcgt ttctcgactg gctggcgcgc accgcgccga cattggatgg
     3481 cgaccgcctg ctggcggccc gcctggcacg ggctccggtg gcgctggggt actacttcag
     3541 cagcgaccgc gctggcagcc gcaccggcgc gctgcccgaa ccctggattc ggccggatcg
     3601 cctcccgagg gggctgctgc actgggatgg ttacggcggc aatatcgccg tgctgaacgg
     3661 cgcggcccgg actgcaggtt tcttcaacgc tgtcgccgac ccagatggca gcgtgcgtgc
     3721 ggtacccctg gtggcggggt tcgaaggggc gttgtatccc tcactctcgt tggccacgct
     3781 gcgccttggc ctgggcgcac ggcaactgcg tgaggagcgc ctcggcccag aggcggacgc
     3841 gccgctccag gctctgtggc tgggtgacga tgtgcaccgt tggcgccttc cggtggatga
     3901 tcgcggaacg gtgctggtgc cctaccgcgg gccgggcggg cctcagggcg ggtcgtttcg
     3961 atacatctcg gccagcgatg tgctgcaggg gcggcttgcg ccggccgaac tcaaggggcg
     4021 ctacgcgctg ctgggcttta ccgctcccgg cttgatggat ctgcgcgcca cccccgtggc
     4081 cgaggtgtac cctggggtcg agattcacgc caacctgatc tccggcatgc tggacgggcg
     4141 cgtggcggtg cggccggact acgcggccgg gtacgaggtc ggcctgctgg tgttgctggg
     4201 cggggtgctc gcgctgggct tgccgctgct gtcggtgggt ggggcgctcg gcctgggcgc
     4261 ggcgctactg gcggccctgc tggccctgga cctcagcctg tacgtcggcg ccggactggc
     4321 cctgccgctg gccacaccgc tggcgctgat cctaattgcg ctgacgctcg atatggcact
     4381 gggctacttc gtcgagggcc gggccaagcg cgccctggcg cgccagttcg ccacctacgt
     4441 gccgcccgaa ctggtgcagc agatgctgca gcagcccgaa cgctacggca tggcggcgcg
     4501 ctcgcaggaa ctgacggtaa tgttctgcga cctgcatggt ttcaccagtc tggcggaaac
     4561 catggagccg cagaccttgc agaccttgct gggcgaagtg ctcagtcacc tgaccgaggt
     4621 gatccagacg catcagggca cgatcgacaa atacatcggc gactgcgtca tggccttttg
     4681 gggcgcgccg gtcgccatgc cggaccacgc gcgacgcgcg gtggatgccg ccgtggccat
     4741 tgtcgccagc ctgcgcgacc tcaacgccga ccacgccgcc cgaggtctgc cggcggtgtc
     4801 ggtgggcatc ggcctgaaca ccggcgtgat gtcggtgggc aacatgggct ccgacctgcg
     4861 gcgcgcctac acggtgatcg gcgacgcggt gaatctggcg gcccggctgg aggcgctgtc
     4921 gcgcgtgtac gaggtcgacc tggtggccag ccaggccacc cgggacgccg ccgcagcggc
     4981 tggccatgtc tggcaggaac tcgatcgggt gcgcgtcaag ggtcgccacc aggcggtcag
     5041 catccacacc gtgcgggcgg cggcgggcca ggacgacgtg gcgttgcagg aggagctggc
     5101 cgcctggcgg caggccctga gcctgtggcg cgccggacac tttgccgagt tcggatcggc
     5161 agtcgaggcg ctgcaggcgc gaaatgccaa ctattgcctt taccggctgt atgccgagcg
     5221 gttagtcttc ttgcttcgtt ttccacccgg tccggattgg gatgggacca cggtgttcga
     5281 cgccaaatga caacaacaac cgaattggtc atcagactgc gcgtgctggg ttgctccggc
     5341 gccattgcgc gcgactgccg caccacgtcg ttcctggtcg atcaggacat cctgatcgac
     5401 gccggcaccg gggtcggcga actcacgcgc gacgagatgc tgcgcatcga gcatgtgttt
     5461 ctgacgcatt cgcacctcga ccatgtggcc tgtctgccgc tgatggtcga caccatggcc
     5521 tgcgatcgca tcggccggcc gattcatgtg catgcgctgc cgcagaccat tgccgcgctg
     5581 cgcgcgcatg tgctcaacga cgtcatctgg ccggatttct cgcgcctgcc cacgcccgag
     5641 ctgccgctga tccagtttca tccgctgcag gtggggcaca cgcgtgagct gggcgggcgc
     5701 gtcatcgagg cgctgccggc gcgccacgcg gtgccggcgc tgggctttgc gctgcgccgt
     5761 caggcgaccg acgcgccctg gtgggtgttc agcggcgaca ccagcgggca cccggacttc
     5821 tggcagcgcc tcaaccagct cagggtgcac agcctggtga tcgaaaccgc cttcgccaac
     5881 cgcgaacgcg agctggcgca gatggcccag cacctgtcgc ccgagacgct ggccgaggag
     5941 ctggaccgga tcgaccgccg gcaccgcttt cccatctaca tcacccacac caagccggcc
     6001 gacaccgaaa ccatcatgcg cgagatcgcc cagttcgacc aggtgcgtcc gccgcgccgc
     6061 gaggtggcgc acgacatccg ctggctcagc gccggccacg tgttcgagtt gtgaccggcg
     6121 cccccgccct cacaggccca ggttaggcgc cagcaggcgc tccagcgccg ccggcgccat
     6181 gccgcggcgt gccgccatgt cggccacctg cgtcatgtcg atgcgtccga cgttgaaata
     6241 gctgctctgc gggtggctga aatagaagcc gctcacgctg gccggcggtg tcatggcgta
     6301 ggactcggtc agccccatgc cgatctcctg cgtgcgcagc agctcgaaca gcgggcgctt
     6361 gacgctgtgg tcggggcagg ccgggtagcc cggggccggg cggatgccgc ggtaccgctc
     6421 ggcgatcagc tcctcgttgc tcaggccttc gtcggcggcg taaccccaga ggtcggtgcg
     6481 cacgcggtgg tgcagccatt cggcgcaggc ctcggccagg cggtcggcca gggccttgag
     6541 cgtgatggcg ctgtagtcgt cgtgcgcggc catgaattcg gccaccttgt cttccacccc
     6601 gatgccggcg gtgacggcaa aggcccccaa atagtccttg aaggcatttt ggcctccagc
     6661 gcccgactgg caagcgccat cagctactga atcaatagca tttcggggga ctacgaaatc
     6721 cgccagacag cggctggggc gcagctggcc ggcggcgtct tcctgcttct cgctctgctg
     6781 gcgcaggccg tgccaggtca gcaccacgtg ctgccgcgcc tcgtcggcgt acagctcgat
     6841 gtcgtcgtcg tgcacgctgt tggccggcca gaagccgacc accgcgttgg cggtgagcca
     6901 gcggccgtcg acgatcttct tcagcatcgt ctgcgcgtcc tggaacacgc gcctggcctc
     6961 ctggcccacc acctcgtcgt tcaggatggc cgggtatttg ccggccagat cccaggtctg
     7021 gaagaacggt gtccagtcga tgaagggcag cagcccggcc agctcgatgt gctggaacac
     7081 gcgccggccg atgaacttgg gcaccggcgg ggtgtagttg ccccagtcga tgcgcgcctt
     7141 gttggcgcgc gcccgctcga tcggccacag cggcgcctgc ttcttgccgg cgtgcagctg
     7201 gcgcacgcgc t
//
LOCUS       DAOOOG010000132         7162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_12397_length_7162_cov_29.068243, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000132 DAOOOG010000000
VERSION     DAOOOG010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12397_length_7162_cov_29.068243"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PK242_09850"
     CDS             <1..827
                     /locus_tag="PK242_09850"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOZ94363.1"
                     /translation="VKKVPLLRGKSVFNLFFENSTRTRTTFDIAATRLSADVYTLDIA
                     RSSTNKGESLLDTVANLSAMAADMFVVRHSESGAPYLLAQHVAPHVHVINAGDGRHAH
                     PTQGLLDMFTIRHYKKDFSNLVVAIVGDVLHSRVARSDIHALTTLGAAEVRVVGPRTL
                     VPGDLAPLGVRVCHTLQEGIQGADVIIMLRLQNERMSGALLPSSQEFFKSFGLTPDKL
                     HWAKPDAIVMHPGPINRGVEIDSRVVDGGQSVILPQVTFGIAVRMAVMSIVAGNEA"
     gene            929..2239
                     /locus_tag="PK242_09855"
     CDS             929..2239
                     /locus_tag="PK242_09855"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOZ94364.1"
                     /translation="MKILIEQGRIIDPASGRDETGDIAIAAGRILSIGRAPADFTPAR
                     RVNARGCWVLPGLVDLAARLREPGHEHARMLESEMAAAVAGGVTSLVCPPDTDPVLDE
                     PGLVEMLKTRAERLHQARVLPLGALTRGLKGEVLTEMGLLTDAGCVAFGQAEAPIVNT
                     QVLQRALQYASSFGYAIWLRPLDPWLGGGVAASGPLAMRMGLAGVPVAAETVALHTVF
                     ELLRAMSGGATAPRVHLCRLSSAAGVALLRAAKAEGLRVTADVSIHSLHLSDEDLGYF
                     DSRARLNPPLRQPRDREALRAALADGTLDALVSDHTPVEADAKQLPFAEAEPGATGLE
                     LLLPLALQWGQAPDGPGIVRALACVTQGPARVLGAALGTLQASCGRLVEGGVADVCVF
                     DPEARWAVDAAALRSQGKHTPFGGLQLTGRVRCTLVAGQLAHSA"
     gene            complement(2296..3147)
                     /locus_tag="PK242_09860"
     CDS             complement(2296..3147)
                     /locus_tag="PK242_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOZ94365.1"
                     /translation="MQAAETASLLPEAPEQAPLPEVVDHVAVARLYGEPLFALPQDLY
                     IPPDALEVFLEAFEGPLDLLLYLIRKQNFNILDIPMAAVTRQYLSYVDEVRSRNLELA
                     AEYLLMAAMLIEIKSRMLLPPKKTAEGQEPEDPRAELVRRLLEYEQMKLAAHRLAELP
                     QYGRDFLRAQVYVEQALKPRFPDVELVELQQAWADILKRARLVQHHKISREELSVREH
                     MSIVLKKLQGRRFALFEDLFDPSRGVPVLVVTLIALLELAKESLVEVTQAEAYAPIYV
                     RLAFTPA"
     gene            complement(3205..3426)
                     /locus_tag="PK242_09865"
     CDS             complement(3205..3426)
                     /locus_tag="PK242_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HOZ94366.1"
                     /translation="MSLARLFQRPHYQSDATDFIDELKRARPELDLQQRQGRALLWDK
                     TVNRELQDEFRAGRVAQQAYVYYAYSDSQ"
     gene            3520..4050
                     /locus_tag="PK242_09870"
     CDS             3520..4050
                     /locus_tag="PK242_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HOZ94367.1"
                     /translation="MSIVKPDFSHPRARALGAVLGAALALAGCDPQAIAELEEGVATE
                     ADVVQRFGQPERVWPETGGARTYEYNRQPEGLRNYMITIGPDGKMSALRQVLTPDNFR
                     RVQPGMGVEEVRRLLGKPARQVPYELRREIVWSWKFLEPPSEKKAFNVVFSPDYRVLR
                     TEVGPDPDGPDMRGGG"
     gene            complement(4047..5780)
                     /locus_tag="PK242_09875"
     CDS             complement(4047..5780)
                     /locus_tag="PK242_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOZ94368.1"
                     /translation="MALAFSFRPRLLDALVGYDRARFVKDLAAGLTVAVVALPLAMAF
                     AIASGLKPEAGIFTAIIAGFLIAALGGSRVQIGGPAGAFIVIVYGIVERYGVANLILA
                     TALSGVLLFLMGLLRLGTLVRFIPIAVIIGFTNGIAVLIALSQVKDFLGLSIAKMPGD
                     FFGILGALWGHLHSFNPWALGVAAASLTVIVGWQRWLPALGPRVQFSGQLSVVPGSIV
                     ALVLATAVVGLFGLPVDTIGSRFGGIPGALPAFAWPAFSWESARFLLMPTITLALLGA
                     IESLLCARVADGMIGDRHDPNQELMAQGVANFITPFFGGMPATGTIARTVTNVKNGAT
                     SPVAGMTHALVLLVVILVAAPLASSIPLACLAAILMHVAWNMGEWREFARLKTYRMPY
                     RVTLVAVFLLTVIFDLTVAVEFGILAACVVFIYRISSLSRGERLTAADQPALAGHDAA
                     LQVWRLYGALFFGATKLVEALEDALPARVLVLDLKNVIYVDSTGAEALENLLQTCRRQ
                     GVRLVVSGLMAQPLDIARRTGLLERLQAQSDQDLQPDVASAIDTAVAGLAPVDAPATA
                     QGDAEPGPART"
     gene            complement(5949..>7162)
                     /gene="metG"
                     /locus_tag="PK242_09880"
     CDS             complement(5949..>7162)
                     /gene="metG"
                     /locus_tag="PK242_09880"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOZ94369.1"
                     /translation="QYHFIGKDIITFHTLFWPAMLKFSGRKVPDNVFVHGFLTVNNGE
                     KMSKSRGTGLDPLKYLELGMNPEWLRYYLAAKLSGRNEDVDFNADDFMARVNADLIGK
                     FVNIASRAAGFISKRFGGRLGEVAPDGDQLLSHLRDAVPQLTTLYAERDYAKVVREVM
                     ALCDRVNAYVDQNKPWELAKQAGMEARLHDVCSCCIEAFRILAICLKPILPRLAAEVA
                     VFTMTPPECFDDALRPLGAGAQIGEYKHLMQRVDSKQLDALFEPPAPAPQALAAIKSI
                     ATESPLVPGGEALAPTIGIDDFARVDLRIARIVECQAVEGSTKLLRLTLDAGEGRTRN
                     VFSGIASAYRPEQLQGKLTVLVANLAPRKMKFGVSEGMVLAASHADEKAQPGIFVLEP
                     WPGAQPGMRVR"
BASE COUNT         1139 a         2459 c         2509 g         1055 t
ORIGIN      
        1 aggtcaagaa ggtgccgctg ctgcgcggca agagcgtgtt caacctgttc ttcgaaaaca
       61 gcacccgcac ccgcaccacc ttcgacatcg ccgccacccg gctgtcggcc gacgtctaca
      121 cgctggacat cgcgcgctcg tcgaccaaca agggcgaatc cctgctcgac acggtggcca
      181 acctgagcgc catggcggcc gacatgttcg tggtgcgcca cagcgaaagt ggcgcgccct
      241 acctgctggc ccagcacgtg gcgccgcatg tgcacgtcat caatgccggc gacgggcgcc
      301 acgcgcaccc gacgcagggg ctgctcgaca tgttcaccat tcgccactac aagaaggatt
      361 tttccaacct ggtggtggcc atcgtgggcg atgtgctgca ttcgcgcgtg gcgcgctccg
      421 acatccacgc cctcaccacc ctgggtgccg ccgaggtgcg ggtggtcggc ccgcgcacgc
      481 tggtgccggg cgatctggcg cccctgggcg tgcgcgtctg ccacacgctg caggagggca
      541 ttcagggcgc cgacgtgatc atcatgctgc gcctgcagaa cgagcgcatg agcggcgcgc
      601 tgctgccgtc gagccaggag ttcttcaaga gcttcggcct cacgcccgac aagctgcact
      661 gggccaagcc cgatgccatc gtcatgcatc ccgggccgat caaccgcggg gtcgagatcg
      721 attcgcgggt ggtcgatggt ggtcagagcg tgatcctgcc gcaggtcacc ttcggcatcg
      781 cggtgcgcat ggcggtgatg agcatcgtgg ccggcaacga ggcgtgaacc gacgcggtgc
      841 ggcatgcctg gcccatcgaa aaagttcgaa aatgatagct gctggcgctt gctggataag
      901 cgccgcagcc tgttttgatg tgtaaaccat gaagatcctg atcgagcaag ggcgcatcat
      961 cgatccggcc tcgggccgtg acgaaacggg cgacatcgcc atcgccgccg gacgcatcct
     1021 gagcatcggt cgggcgccgg ccgacttcac gcccgcccgc cgcgtcaatg cgcgcggctg
     1081 ctgggtgttg cccggtctgg tcgatctggc ggcgcgcctg cgtgagcccg gccacgagca
     1141 cgcgcgcatg ctcgaaagcg aaatggccgc cgcggtggcg ggcggcgtca ccagcctggt
     1201 ctgcccgccc gacaccgatc cggtgctgga cgagcccggc ctggtcgaaa tgctcaagac
     1261 ccgcgccgag cgcctgcatc aggcgcgcgt gctgccgctg ggcgcgctca cgcgtggcct
     1321 caagggcgag gtgctgaccg aaatgggcct gctcaccgac gccggctgtg tggccttcgg
     1381 gcaggccgag gcgcccatcg tcaacaccca ggtgctgcag cgcgcgctgc aatatgccag
     1441 cagcttcggc tacgcgatct ggttgcgccc gctcgacccc tggcttggcg gcggcgtggc
     1501 cgccagtggg ccgctggcga tgcgcatggg gctggccggc gtgccggtgg cggccgaaac
     1561 cgtggccctg cacaccgtgt tcgagctgct gcgcgccatg tcgggcggcg ccacggcgcc
     1621 gcgcgtgcac ctgtgccggc tgtccagcgc ggccggcgtg gcgctgctgc gcgccgccaa
     1681 ggccgagggc ctgcgcgtga cggccgacgt cagcatccat tcgctgcacc tgagcgacga
     1741 ggacctgggc tacttcgaca gccgcgcccg cctcaacccg ccgctgcgcc agccgcgtga
     1801 ccgcgaagcg ctgcgggcgg cgctggccga cggcaccctg gacgccctgg tgtccgacca
     1861 caccccggtc gaggccgacg ccaagcagtt gccgtttgcc gaagccgagc ccggcgccac
     1921 cgggctggag ctgctgctgc cgctggcgct gcaatggggc caggcgccgg acgggcccgg
     1981 catcgtgcgc gcgctggcct gcgtcaccca ggggccggcc cgggtgctgg gcgcggcgct
     2041 gggcaccttg caggccagtt gcggccgcct ggtggaaggc ggcgtggccg acgtctgcgt
     2101 gttcgacccc gaagcgcgct gggccgtcga tgcggccgcg ctgcgcagcc agggcaagca
     2161 cacgccgttt ggcggcctac agctgaccgg ccgggtgcgc tgcaccctgg tggcagggca
     2221 gctggcgcac agtgcctgag cctgggccgg gccgcgcacg cgggcgttgc gcgccgccgt
     2281 cgggcgcgcc cgggctcagg cgggcgtgaa cgccagccgc acgtagatgg gcgcgtaggc
     2341 ctcggcctgg gtgacctcga ccaggctttc cttggccagc tccagcagcg cgatcagcgt
     2401 caccaccagc accggcacgc cgcggctggg gtcgaacagg tcctcgaaca gcgcaaagcg
     2461 ccggccctgc agcttcttga gcacgatcga catgtgctcg cgcacgctca gttcttcgcg
     2521 cgagatcttg tggtgctgca ccagccgggc gcgcttgagg atgtcggccc aggcctgctg
     2581 cagctccacc agttcgacgt cgggaaagcg cggtttgagc gcctgctcca catacacctg
     2641 ggcacgcagg aagtcgcgcc cgtactgcgg cagctcggcc agccggtggg cggccagctt
     2701 catctgctcg tattcgagca ggcggcgcac cagctcggcg cgcgggtcct cgggctcctg
     2761 gccttcggcc gtcttcttgg gcggcagcag catgcgcgac ttgatctcga tcagcatggc
     2821 ggccatcagc aggtactcgg ccgccagctc caggttgcgg ctgcgcacct cgtcgacgta
     2881 gctcaggtac tggcgcgtca ccgccgccat cgggatgtcg aggatgttga agttctgctt
     2941 gcggatcagg tacagcagca ggtccagcgg gccttcgaag gcctccagaa agacctccag
     3001 cgcatcgggc gggatgtaca gatcctgcgg cagcgcgaac agcggctcgc cgtacaggcg
     3061 cgccaccgcc acgtggtcca ccacctcggg cagcggcgcc tgctcaggcg cctcgggcag
     3121 caaggacgcg gtttcggcag cctgcatgaa tgctactgaa aagatagctt gtggcgctta
     3181 cttggcgggc gccacagccg cttttcattg tgaatcgctg taggcgtagt acacgtaggc
     3241 ctgctgcgcc acacggccgg cgcggaactc gtcctgcagt tcgcgattga ccgtcttgtc
     3301 ccacagcagg gcgcgcccct ggcgctgctg cagatccagc tcgggccggg cgcgcttgag
     3361 ctcatcgatg aaatcggtgg cgtccgactg gtaatgcggg cgctgaaaca ggcgggcaag
     3421 ggacatacac tggagctttc gtgatcggtg ccacggaggc tggcacaggc gccgcgcggg
     3481 ccaccggcac gcacgcgtca accgatcatt ttaccgggca tgagcatcgt gaaacctgac
     3541 ttttcccatc cgcgcgcgcg ggcgctgggc gccgtgctgg gggccgcact ggcgctggcg
     3601 ggctgcgacc cgcaggccat tgccgaactg gaagagggcg tggccaccga agccgacgtg
     3661 gtgcagcgct tcggccagcc cgagcgggtg tggccggaga ccggcggtgc cagaacctac
     3721 gaatacaacc gtcagcccga gggcctgcgc aactacatga tcaccatcgg ccccgacggc
     3781 aagatgagtg cgctgcgcca ggtgctgacg cccgacaact tccgccgcgt gcagcccggc
     3841 atgggcgtcg aggaggtgcg ccgcctgctc ggcaagccgg cccggcaggt gccctacgag
     3901 ctccggcgcg aaatcgtctg gagctggaag ttcctggaac cgccgagcga gaagaaggcc
     3961 ttcaacgttg tcttcagccc cgactaccgc gtgctgcgca ccgaagtcgg ccccgacccg
     4021 gacgggcccg acatgcgggg cggcggttag gtccgcgcgg ggcccggctc ggcgtcgccc
     4081 tgggcggtcg cgggggcatc gacgggcgcc aggcctgcca ctgccgtgtc gatggcgctg
     4141 gccacatcag gctgcagatc ctggtccgac tgcgcctgca gccgctccag cagcccggtg
     4201 cggcgcgcga tgtccagcgg ctgcgccatc aggcccgaga cgaccagccg cacaccctga
     4261 cggcggcagg tctgcagcag gttttccagc gcctcggcgc cggtcgaatc gacgtagatg
     4321 acgttcttca ggtccagcac caggacgcgc gcgggcagtg cgtcctccag cgcttcgacc
     4381 agcttggtgg cgccgaagaa cagcgccccg tacaggcgcc agacctgcag cgccgcgtcg
     4441 tggccggcca gcgccggctg atcggcggcc gtcaggcgct cgccccgcga caggctggag
     4501 atgcggtaga tgaacaccac gcaggccgcc aggatgccga actccaccgc caccgtgaga
     4561 tcgaagatca ccgtcagcag gaacaccgcc accagggtga cgcggtaggg catgcggtag
     4621 gtcttcaggc gcgcgaactc acgccactcg cccatgttcc aggccacatg catcaggatg
     4681 gccgccaggc aggccagcgg gatggagctg gccagcggcg ccgccaccag gatcaccacc
     4741 agcagcacca gcgcatgcgt catgcccgcg acggggctgg tggcgccgtt cttcacgttg
     4801 gtgacggtgc gcgcaatcgt gcccgtggcc ggcatgccgc cgaagaacgg cgtgatgaag
     4861 ttggccacgc cctgggccat cagctcctgg ttggggtcgt ggcggtcgcc gatcatgccg
     4921 tcggccacgc gcgcgcacag cagcgactcg atggcgccca agagcgccag cgtgatggtg
     4981 ggcatgagca gaaagcgcgc cgactcccag ctgaacgccg gccaggcgaa ggccggcagc
     5041 gcgcccggta tgccgccaaa gcggctgccg atggtgtcca ccggcaggcc gaacagaccc
     5101 accactgccg tcgccagcac cagcgccacg atggagcccg gcaccacgct gagctggccc
     5161 gagaactgca cccgcggccc cagtgccggc agccagcgct gccagccgac gatgaccgtc
     5221 agcgaggccg ccgccacgcc cagcgcccag gggttgaagc tgtgcaggtg gccccacagc
     5281 gcacccagga tgccgaagaa gtcgcccggc attttggcaa tcgacaggcc gagaaaatcc
     5341 ttcacctgcg acagcgcgat cagcaccgcg atgccgttgg tgaagccgat gatcaccgcg
     5401 atcgggatga agcgcaccag cgtgcccagg cgcagcagcc ccatcaggaa cagcagcacg
     5461 cccgacagcg ccgtggccag gatcaggttg gccacgccgt agcgctcgac gatgccgtag
     5521 acgatgacga tgaaggcgcc cgccggcccg ccgatctgca cgcggctgcc gcccagcgcc
     5581 gcgatcagaa agccggcgat gatggcggtg aagatgccgg cctcgggctt gagcccgctg
     5641 gcgatggcaa acgccatggc cagcggcagc gccaccacgg ccaccgtgag gccggcggcc
     5701 aggtccttga cgaagcgcgc acggtcatag ccgaccagag catcgagcag acggggacga
     5761 aaggaaaaag ccagggccaa gttcgactcc aggaagacgt gagcgacaac gcaggcaagc
     5821 ggtgtgttca gggccttccc ggtgggccat ggtgatgccg ccggatcatg aaccggcggc
     5881 ggcgcgaaag cggcaggatc ggctggcgtg ccatggcagc gtcagtgtac gccgccgcgg
     5941 cgccgcgttc agcgcacgcg catgccgggc tgcgcgcccg gccagggctc gagcacgaag
     6001 atgccgggct gcgccttctc gtcggcgtgg ctggcggcca gcaccatgcc ttcgctgacg
     6061 ccgaacttca tcttgcgcgg cgccaggttg gccaccagca cggtgagctt gccctgcaac
     6121 tgctcgggcc ggtaggcgct ggcgatgccg ctgaacacgt tgcgcgtgcg gccctcgccg
     6181 gcgtcgagcg tcaggcgcag cagcttggtc gagccttcga ccgcctggca ctcgacgatg
     6241 cgggcgatgc gcagatcgac ccgggcgaag tcgtcgatgc cgatggtggg cgccagcgcc
     6301 tcgccgcccg gcacgagggg gctttcggta gctatcgatt taatagctgc cagcgcttgc
     6361 ggggcgggcg ccggaggctc aaaaagtgca tccagctgct tgctgtccac gcgctgcatc
     6421 aggtgcttgt actcgccgat ctgcgcgccc gcgcccagcg ggcgcagggc gtcgtcgaag
     6481 cactcgggcg gcgtcatggt gaacaccgcc acctcggccg ccagccgcgg caggatgggc
     6541 ttgaggcaga tcgccaggat gcggaaggcc tcgatgcagc agctgcacac gtcgtgcagg
     6601 cgcgcttcca tgccggcctg cttggccagc tcccagggct tgttctggtc gacgtaggcg
     6661 ttgacgcggt cgcacagcgc catcacctcg cgcaccacct tggcgtagtc gcgctcggcg
     6721 tacagcgtgg tgagctgggg cacggcgtcg cgcaggtggc tcagcagctg gtcgccgtcg
     6781 ggggcgacct cgcccagccg gccgccgaag cgcttgctga tgaagccggc cgcgcgcgag
     6841 gcgatgttga cgaacttgcc gatcaggtcg gcgttgacgc gcgccatgaa gtcgtcggcg
     6901 ttgaaatcga cgtcctcgtt gcgccccgac agcttggcgg ccaggtagta gcgcagccac
     6961 tcggggttca tgcccagttc gaggtacttg agcggatcca gcccggtgcc gcggctcttg
     7021 ctcatcttct cgccgttgtt gacggtcaga aagccgtgca cgaacacgtt gtcgggcacc
     7081 ttgcggccgc tgaacttgag catggcgggc cagaacagcg tgtggaaggt gatgatgtcc
     7141 ttgccgatga agtggtattg ct
//
LOCUS       DAOOOG010000133         7138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_12448_length_7138_cov_28.168996, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000133 DAOOOG010000000
VERSION     DAOOOG010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12448_length_7138_cov_28.168996"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PK242_09885"
     CDS             complement(<1..389)
                     /locus_tag="PK242_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HOZ94370.1"
                     /translation="MNLRPLTPTELRVLGTLMEKARTVPDTYPLSLNAVVSGCNQKTS
                     REPVMNLSEAEAQAALEALKALHLAIESHGSRVARWEHNLQRALTVPEQSAVLLGLLM
                     LRGPQTAAELRLNAERWYRFADISSVEG"
     gene            414..995
                     /gene="purN"
                     /locus_tag="PK242_09890"
     CDS             414..995
                     /gene="purN"
                     /locus_tag="PK242_09890"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOZ94371.1"
                     /translation="MKHIVILISGTGSNMAAIVRAAEREDWAARLGARVAAVISNRPD
                     ARGLQTAQEAGIATAVVDHRQHADRAAFDAALRAEIDRHQPALVVLAGFMRILTPGFV
                     AHYAGRIVNIHPSLLPAFPGLHTHQRAIDMRCRVAGATVHQVTAELDHGAILAQAAVP
                     VLPGDTADTLAARVLTQEHLIYPRAIAELLSKG"
     gene            1197..2462
                     /locus_tag="PK242_09895"
     CDS             1197..2462
                     /locus_tag="PK242_09895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOZ94372.1"
                     /translation="MHPKALLDAASELVLRVLRLDHPADAVVSRFFREQRQLGPRERA
                     TLAETAYAVLRRKPLFEHLARSGSGGRERRLAILGFAAPRDFLKQALTDAEKHWLDAC
                     DAIAPAELPEACRHNLPAWLVEPLRAQLGDGFDALAQALLQGAPLDLRVNTMLQKREL
                     AAQELQALGIDSVPTPYSPWGLRLAGKPALTRLPPFQQGRIEVQDEGSQLLALLLDAR
                     RGEMVTDFCAGAGGKTLAIGAAMRSTGRLYAFDVSAHRLEALKPRLARSGLSNVHPVA
                     IAHERDERIKRLAGKMDRVLVDAPCSGLGTLRRNPDLKWRQTPEAVAELVSKQRAILA
                     SAARLLKPGGRLVYATCSLLTEENEAIAAAFGGEHTAFQPLPVAELLTGLKVTGAAAL
                     CTADGAYLRLWPHRHATDGFFAAAWQRRA"
     gene            2547..3440
                     /locus_tag="PK242_09900"
     CDS             2547..3440
                     /locus_tag="PK242_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOZ94373.1"
                     /translation="MARDIRTVVSGMHFLEGPRWHDGRIWFSDFYSFQVYSAREDGSD
                     LRVEARVEHKPSGIGWLPDGRLLVVSMRDRKLLRREADGTLVTHADLSDYAGGNANDM
                     VVDAQGRAWVGDFGFDLMGGAPFRPTGLWRVDPDGTVHRAAEDMWFPNGAVITDDGTL
                     RVCETFANRVTAFDIGADGTLSRRRVWARFGEPPTSADVGEAIGQVAVAGDGCCLDAE
                     GAMWIADAVHGRVVRVREGGQIVDEIQPGTGAFACMLGGADGRTLFICAAPDFYEHAR
                     AAATEASLLSVRVDVPHAGRP"
     gene            3464..4615
                     /locus_tag="PK242_09905"
     CDS             3464..4615
                     /locus_tag="PK242_09905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ94374.1"
                     /translation="MVEADDAVLIIGAGIAGASLAWRLARAGRPVTLIEREPQPGMHS
                     TGRSAAMFMASYGPPGVRALTRASLDFYLQPPAGFAEQPLLSPRRALFVATAEQQPAL
                     ARMRADLTASGTTTTLLNSEQLALSAPALRPGMFQNALLDAQGYDIDVHALLQGFLRG
                     ARQAGARLRTGVEPQLARHDGQRWQVTLSDGSAWTGRTVVDAAGAWADQVAVLFGAAP
                     IGLEPRRRSAFTFRAPAGADLGQWPMVADVDEAWYFKPDAGQLLGSPANADPTIPHDV
                     QPEDYDIALGIHLIQEATTLRIDRPTATWAGLRSFVADGDLVIGFDQACPGFFWLAAQ
                     GGYGIQSAAGASLLAASQIVGEPLPAELAAHGVDEAVLSPRRLRRTAPS"
     gene            complement(4662..5294)
                     /locus_tag="PK242_09910"
     CDS             complement(4662..5294)
                     /locus_tag="PK242_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ94375.1"
                     /translation="MLSDPQAFAWPADASTVAAGTQLLRRACVDEHVLYIDSGRVALG
                     VVENGVLAHQMGLVEGPAWLNVGCAVLDKAPVVDAVADSPVQLRRVPLRDFVAGLREM
                     PAPAQALLRDMAAAHRQQAELAVSRLAKDAEARCAEWLLRHAEPAQGAPGTLAVLLRQ
                     RKRMIAAQLGIAPETLSRVLRQLRERSLISGSGRILNLLDPGALRALAGA"
     gene            complement(5409..5484)
                     /locus_tag="PK242_09915"
     tRNA            complement(5409..5484)
                     /locus_tag="PK242_09915"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5449..5451),aa:Thr,seq:cgt)
     gene            complement(5598..6299)
                     /locus_tag="PK242_09920"
     CDS             complement(5598..6299)
                     /locus_tag="PK242_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HOZ94376.1"
                     /translation="MNDRTQTLAYLDATRGGVASQEQRHRVLRNTYWLLALSLLPTVL
                     GAWLGVATGITAGLGGGLGLVVFLGGAFGFMFAIEKTKNSAAGVPVLLAFTFFMGLML
                     SRLIALVLGFKNGSELVMTAMGGTAGVFFVMASLASVIKRDLSGLGKWLFVGALVVFV
                     GAIVNVFVGSTVGMLVISTLVIAVFSLYMLYDLKRIVDGGETNYISATLALYLDLFNV
                     FQGLLALLGLGGSND"
     gene            6445..6849
                     /locus_tag="PK242_09925"
     CDS             6445..6849
                     /locus_tag="PK242_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOZ94377.1"
                     /translation="MKTKPVLQAADVRAIVAAAEAEALKQQWAMTIAVADDGGHLLHL
                     HRMDGAAPVSAHIAPGKARSAALGRRESKGYEDMINGGRHAFLSVPLEGMLEGGLPIV
                     KDGQCIGAIGVSGAKPAEDTLVARAGIAALGL"
BASE COUNT         1070 a         2538 c         2467 g         1063 t
ORIGIN      
        1 ccttcgaccg aggagatgtc ggcaaagcgg taccagcgct cggcgttcag ccgcagctcg
       61 gcggcggtct gcgggccgcg cagcatcaaa aggcccagca gcaccgccga ctgctcgggc
      121 acggtcaggg cgcgctgcag gttgtgttcc cagcgtgcca cgcggctgcc gtgcgactcg
      181 atcgccaggt gcagcgcctt cagcgcctcc agcgcggctt gcgcctcggc ttcgctcagg
      241 ttcatcaccg gctcgcggct ggtcttctgg ttgcagccgc tcaccacggc gttgagcgac
      301 agggggtagg tatcgggcac ggtgcgtgcc ttttccatca gcgtgcccag cacgcgcagc
      361 tcggtcgggg tcaggggacg caggttcatc ggccaaggat aatccggccc gacatgaaac
      421 acatcgtgat cctgatctcc ggcaccggca gcaacatggc ggccatcgtg cgcgccgccg
      481 aacgcgaaga ctgggccgcc cggctgggcg cgcgcgtggc ggcggtcatc agcaaccggc
      541 ctgacgcccg gggtctgcag accgcgcaag aggcgggcat cgccaccgcc gtggtcgatc
      601 acaggcagca tgccgaccgc gccgccttcg acgccgcgct gagggccgag atcgaccgcc
      661 atcagccggc gctggtggtg ctggccggct tcatgcgcat cctcacgccc ggcttcgtgg
      721 cgcactacgc ggggcgcatc gtcaacatcc accccagcct gctgcccgcc tttccgggcc
      781 tgcacacaca ccagcgcgcc atcgacatgc gctgccgcgt ggccggcgcc acggtgcatc
      841 aggtgacggc cgagctggac cacggcgcca ttctggcgca ggccgcggtg ccggtgctgc
      901 cgggcgatac cgcggacacc ctggccgccc gggtgctgac gcaggagcac ctgatctatc
      961 cgcgcgcaat cgccgaattg ctatctaaag gatagctacc tgcgcaattt gatagagcgc
     1021 tggagccaaa tttggttcat agtcggtatc tctgaatccc agccagactc caggcgaaga
     1081 ctccgaaggc atcccacaag ccgtttgttt tcttttgcgt cctctgcgaa ccctctgcgc
     1141 gctcggcgta agggtgttct cgtccattca acccactccc gttccagccg cccaccatgc
     1201 accccaaagc cctgctcgac gccgccagcg aactggtgct gcgcgtgctg cgcctggatc
     1261 acccggcgga cgccgtggtc tcgcgctttt tccgcgagca gcgccagctc ggcccgcgcg
     1321 agcgtgccac cctggccgaa accgcttacg ccgtgctgcg tcgcaagccg ctgttcgagc
     1381 acctggcgcg ctcgggctcg ggtggacgcg agcggcgcct ggcgattctg ggttttgctg
     1441 caccccgtga ctttctcaaa caggccttga ccgacgccga aaagcactgg ctcgatgcct
     1501 gcgacgccat cgcccccgcc gagctgcccg aggcctgccg ccacaacctg ccggcctggc
     1561 tggtcgagcc gctgcgcgca cagctgggtg atgggttcga cgccctggcg caggccctgc
     1621 tgcagggcgc gccgctggac ctgcgcgtca acaccatgct acaaaaacgg gagctggcgg
     1681 cgcaggaact gcaagcgctg ggcatcgatt cggtgcccac cccttactcg ccctggggtc
     1741 tgcgtctggc cggcaagccg gcactgacgc ggctgccacc ctttcagcag ggccgcatcg
     1801 aggtgcagga cgaaggctcg cagctgctgg ccctgctgct ggacgcgcgg cgcggcgaaa
     1861 tggtgaccga cttctgcgcc ggtgccggcg gcaagacgct ggccattggc gcggccatgc
     1921 gctccaccgg gcggctgtac gctttcgatg tgtcggcgca tcggctggag gcgctcaagc
     1981 cgcggctggc gcgcagcggc ctgtccaacg tgcacccggt ggccattgcg catgagcgcg
     2041 acgagcgcat caagcgcctg gccggcaaga tggaccgcgt gctggtcgat gcgccgtgct
     2101 cgggcctggg cacgctgcgc cgcaaccccg acctcaaatg gcgccagacg cccgaggcgg
     2161 tggccgagct ggtgagcaag cagcgcgcca tcctggccag cgctgcgcgc ctgctcaagc
     2221 ccggcgggcg gctggtgtac gccacctgca gcctgctgac cgaggagaac gaggccatcg
     2281 ctgcagcgtt cggtggcgag cacaccgcgt ttcagccgct gccggtggcc gagctgctga
     2341 ccggcctgaa ggtgaccggt gcggcggcgc tgtgcacggc cgatggtgcc tacctgcgcc
     2401 tgtggccgca ccggcatgcc accgacggct tctttgccgc cgcctggcag cgccgcgcct
     2461 gacgcctgcg ggaccacccg gcgcttggac ggcgcggcac gcggcgtcac aatcggtcgc
     2521 atccatcacc gacaaaggag cgtttcatgg cacgcgacat tcgcaccgtg gtttcgggca
     2581 tgcactttct cgaagggccg cgctggcacg atggccgcat ctggttctcc gacttctatt
     2641 cgttccaggt ctattcggcg cgcgaagacg gcagcgacct gcgcgtcgag gcgcgcgtcg
     2701 agcacaagcc ctcgggcatc ggctggctgc ccgacgggcg gttgctggtg gtgtccatgc
     2761 gcgaccgcaa gctgctgcgg cgcgaggccg acggcacgct ggtgacgcac gccgacctgt
     2821 cggactacgc cggtggcaac gccaacgaca tggtggtcga cgcccagggc cgcgcctggg
     2881 tgggcgactt cggcttcgac ctgatgggcg gcgcgccttt tcgccccacc ggcctgtggc
     2941 gggtcgaccc cgacggcacg gtacaccgcg ccgccgaaga catgtggttt cccaacggcg
     3001 ccgtgatcac cgacgacggc acgctgcgcg tgtgcgaaac ctttgccaac cgggtcacgg
     3061 ccttcgacat cggcgccgac ggcacgctca gccgccgccg cgtctgggcc aggttcggcg
     3121 agccgcccac cagcgccgac gtgggcgaag cgatcggcca ggtggcggtg gccggtgacg
     3181 gctgctgcct ggacgccgaa ggcgccatgt ggattgccga cgccgtgcac ggccgcgtgg
     3241 tgcgggtgcg cgagggtggc cagatcgtcg acgagatcca gcccggcacc ggcgccttcg
     3301 cctgcatgct gggcggtgcc gacggccgca cgctgttcat ctgcgccgcg cccgacttct
     3361 acgagcacgc gcgcgccgcc gccaccgagg cgagcctgct gtcggtgcgc gtggacgtgc
     3421 cgcacgccgg ccggccctga gcgccgaagc gagggcacgc cccatggtcg aagcggacga
     3481 cgcggtgctc atcatcggcg ccggcatcgc cggcgcttcg ctggcctggc ggctggcgcg
     3541 cgccggccgg ccggtgacgc tgatcgagcg cgagccgcag cccggcatgc acagcaccgg
     3601 ccgttcggcc gccatgttca tggcaagcta cggcccgccc ggggtgcgcg cgctgacccg
     3661 cgccagcctc gacttctacc tgcagccgcc ggccggtttt gccgagcagc cgctgctgag
     3721 cccgcgccgc gccctgttcg tcgccaccgc cgagcagcag cccgcgctgg cgcgcatgcg
     3781 ggccgatctg acggccagcg gcaccaccac cacgctactg aattcagagc agctggcgct
     3841 gtctgcgcct gccctgaggc ccggtatgtt tcagaacgcc ctgctggatg cgcagggtta
     3901 cgacatcgat gtccacgccc tgttgcaagg ttttctgcgc ggcgcccgcc aggccggcgc
     3961 gcgcctgcgg actggcgtcg agccgcagct tgccaggcac gacggccagc gctggcaggt
     4021 gacgctgtcg gacggcagcg cctggacagg gcgcaccgtg gtcgatgcgg ccggcgcctg
     4081 ggccgaccag gtggcggtgc tgttcggcgc cgcacccatc ggcctggagc cgcgccggcg
     4141 cagcgccttc accttccgcg cgccagccgg cgccgacctg ggccagtggc cgatggtggc
     4201 cgacgtcgat gaagcctggt atttcaaacc cgacgccggc cagttgctgg gctcacccgc
     4261 caacgccgac cccaccatcc cgcatgacgt gcagcccgaa gactatgaca tcgccctggg
     4321 catccatctg atccaggaag cgaccacgct gcgcatcgac cgcccgaccg ccacctgggc
     4381 cggcctgcgc agcttcgtgg ccgatggcga tctggtgatc gggtttgacc aggcttgccc
     4441 aggcttcttc tggctggccg cgcagggcgg ctacggcatc cagagtgcgg ccggcgccag
     4501 cctgctggcc gccagccaga tcgtgggcga gccgctgccg gccgagctgg cggcccatgg
     4561 ggtcgatgag gctgtgctgt cgccgcgccg gctgcgccgc accgcgccgt cgtagcgcgc
     4621 gccccggctg cggggcgggg cagctggctt gcggtggtgg ctcaggcgcc tgccagcgcc
     4681 cgcagagcgc ccggatcgag caggttcagg atgcggccgg agccgctgat caggctgcgc
     4741 tcgcgcaact ggcgcagcac gcgcgacagg gtttcgggcg cgatgcccaa ctgcgcggcg
     4801 atcatgcgct tgcgctggcg cagcaacacg gccagcgtgc cgggcgcgcc ctgcgccggc
     4861 tcggcatggc gcagcagcca ttcggcgcag cgcgcctcgg cgtccttggc cagccggctg
     4921 accgccagtt cggcctgctg gcggtgcgcc gccgccatgt cgcgcagcag cgcctgggcc
     4981 ggcgccggca tctcgcgcag cccggccacg aagtcgcgca gcggcacgcg ccgcaactgc
     5041 accgggctgt cggccacggc gtccaccacc ggcgctttgt ccagcacggc gcagccgacg
     5101 ttcagccagg ccgggccttc aaccagaccc atctggtgcg ccagcacgcc gttttcgacc
     5161 acccccagcg ccacgcggcc actgtcgatg tacaacacat gctcgtcgac gcaggcgcgc
     5221 cgcagcaact gggtgccggc cgccaccgtg ctcgcatcgg cgggccaggc aaaggcttga
     5281 ggatcggaca acatgcgggc actgtgccgc gccgcaaccg gtggtgcttt gatgcacatc
     5341 aacggggcgc accaccacac ccgccaaaga caaggcccct ggggaaaacc aggggccttc
     5401 ggggggtttg gtgccggctg ccggaatcga actggcgacc tacgcgttac gagtgcgttg
     5461 ctctaccaac tgagctaagc cggcgaagac ttgaaagccg cgcattatag ccggatcagg
     5521 gtaccctgag acggtaccgg cgtggcgccc gtgcgccacc ccatgcacga tccggcggca
     5581 agcttgcagg ccgtcgatca gtcgttgctg ccacccaggc cgagcagggc cagcaggccc
     5641 tggaacacgt tgaacaggtc caggtacagc gccagcgtgg cgctgatgta gttggtttcg
     5701 ccgccgtcga cgatgcgctt gaggtcgtac agcatgtaca ggctgaacac ggcgatcacc
     5761 agggtgctga tgaccagcat gccgaccgtg gagccgacga acacgttgac gatggcgccg
     5821 acgaagacca ccagggctcc cacgaacagc catttgccca ggcccgacag gtcgcgtttg
     5881 atgacgctgg ccaggctggc catgacgaag aacacgccgg ctgtgccgcc catggcggtc
     5941 atcaccagtt ccgagccgtt cttgaagccg agcaccaggg cgatcagacg cgacagcatc
     6001 aggcccatga agaaggtgaa ggccagcagc acgggcacgc cggcggccga gttcttggtt
     6061 ttttcgatcg cgaacatgaa gccgaaggcg ccgcccagaa acaccaccag acccaggcct
     6121 ccgcccaggc cggccgtgat gccggtggcc acgcccaacc aggcgcccag cacggtgggc
     6181 agcaggctca gcgccagcag ccagtaggtg ttgcgcagca cgcggtggcg ctgctcctgg
     6241 cttgccacgc cgccccgggt ggcgtccagg taggccaggg tctgggtgcg atcgttcatg
     6301 tctcggttgc tccttggata cccgctgcgg ccgccacagc ggcgaccttc gggggcattt
     6361 tacgtagagt cggtgccggc gcatcggttc cctggccggc ggttatgctt gcgggcatcc
     6421 cgttcaagcc tggagcccgc acccatgaag accaaacctg tgctgcaagc cgccgacgtg
     6481 cgcgccatcg ttgccgccgc cgaagccgag gcgttgaagc agcaatgggc catgaccatc
     6541 gccgtggccg acgacggcgg ccatctgctg cacctgcacc gcatggacgg tgcggcgccc
     6601 gtgagcgccc acatcgcgcc gggcaaggcg cgcagcgctg cgctggggcg gcgcgagagc
     6661 aagggctacg aggacatgat caacggtggc cgccacgcct ttctgtcggt gccgctggag
     6721 ggcatgctgg aaggcgggct gccgatcgtc aaggacggcc agtgcatcgg tgccatcgga
     6781 gtgagcggtg ccaaacccgc ggaggacacg ctggtggccc gggccgggat cgcggcgctc
     6841 ggtctctgag ttcaggacgg ccgccgggcg cgccccccgg tgtcgcgctg gccgagccag
     6901 gcctgggcgc tgccgccgcg aagctggtgc ccgcaggatc cgggtccaga ggctcgattc
     6961 ggggtcagaa cagcctccag cgcttgccag acaagcgcca aaagctatga aaaggatagt
     7021 cttcgtggcg ctccaggcgc cgcgtcggag ggcgttcagt agctgagccg ctcgggccgc
     7081 acttcctgca ggatggtggt ggcgatttcc tcgatcgact tggtggtcga cgacagcc
//
LOCUS       DAOOOG010000134         6954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_12872_length_6954_cov_29.404551, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000134 DAOOOG010000000
VERSION     DAOOOG010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12872_length_6954_cov_29.404551"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PK242_09930"
     CDS             <1..731
                     /locus_tag="PK242_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ94378.1"
                     /translation="NLIGFPLLVALALRSVTAVHGAVVLAVLPLATAAAAALLLGQRE
                     RLGFWLCALVGAALVVLYAWLRAQAAGGFGFAAADLLLLGAVLAAAIGYVAGAQLTPQ
                     LGAERVICWMCVLGLPLSLPLVAWWWPEAQTMAQVRAPAWAGLVYVGAVSMWSGFFAW
                     FRGLDWGGALRVSQVLLLQPFLTMLFAVPLLGERLDALSLAFAAAVVLTVFVGRRLSR
                     PTQAGAPLNGREPQTLSPPKGQQP"
     gene            728..1954
                     /locus_tag="PK242_09935"
     CDS             728..1954
                     /locus_tag="PK242_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOZ94379.1"
                     /translation="MTQSPWQLAARAETMNPSAIREILKITERPGILNFAGGLPAPET
                     FPVDAMRAACAAVLAEGSAVARPSLQYAASEGLPELRQWVAAELGRQGARVVPEQVLI
                     TNGSQQGLDLVAKVLLDAGSRVLVETPTYLGALQAFTPMQPRFDSVDSDDGGVTASGL
                     REQLQKRERPRFVYLLPNFQNPSGRTQDEARRQQVIAVCREHALPIVEDNPYGELWYD
                     QPPPAPLLARWPEGVIYLGSFSKILAPGLRLGYVVAPPSLYPKLLQAKQAADLHTAGF
                     NQRVVAEVIRDGFLDRHVPTIRARYKTQRDAMLAALAHHLDGLGAVWTQPAGGMFIWV
                     RLPEGLNAQTLLPAAVEAGMAFVPGAPFFAGQSDVRTLRLSFVTSTPAQIERGMAALA
                     SVVRAAMSGTGSGSGA"
     gene            2002..2904
                     /locus_tag="PK242_09940"
     CDS             2002..2904
                     /locus_tag="PK242_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOZ94380.1"
                     /translation="MARPFAQIDVFTARPGWGNPVAVVLDGQGLDDAAMQRFARWTNL
                     SETTFLLPPTAAGADYALRIFTPGGELPFAGHPTLGSCHSWLAAGGRPGTPGRIVQQC
                     AQGLVELRQHGQRLAFAAPPLRRGDVPPALLTRVVQALGLAPAQVRRAQWLDNGPRWL
                     GLLLDGADTVLALEPDHAALAALGQKVGVAGVHGTPTAIPLIARSSREARAFRPDDDA
                     APRLEVRAFAAPIGVPEDPVTGSLNASLAQWLIDAGELPARYVAAQGAALGRAGRVHV
                     QRDAQGQVWVGGDCVAVIGGEVRL"
     gene            2901..3575
                     /locus_tag="PK242_09945"
     CDS             2901..3575
                     /locus_tag="PK242_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ94381.1"
                     /translation="MSARLDHMMVLADTLAQGVAWCEATLGVVPAPGGQHPLFGTHNR
                     LLALGGAAFAQAYLEIIAIDPAAGDPGRTRWFDMDDQRLRRQVRAQGPQLIHWVARVP
                     ELARAAAAWAALGLERGAVLPASRMTPRGLLEWQITVREDGQRLLDGCLPTLIQWGAV
                     HPADALPASGIALESLSLRHPQAALLRAALDAVGLAGVAVEPGPAALQARLATPRGAV
                     TLAGFS"
     gene            3637..4611
                     /locus_tag="PK242_09950"
     CDS             3637..4611
                     /locus_tag="PK242_09950"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HOZ94382.1"
                     /translation="MSSEPTLPSLAEIEAAAQVVYRAMPPTPQYRWALLSERLGADCW
                     VKHENHGPVGAFKLRGGLTFFDALARRGQLPREVISATRGNHGQSIGWAARAHGVHCT
                     IVVPRGNSLEKNAAMRALGVELIEQGEDFTAAAEHARRLADERGALLVPSFHRDLVLG
                     VATYWWEFLRAVPRLDVAYVPIGLGSGACAALAARRALGHPARIVGVVSRLAPTYALS
                     LAAGRVVEAPATTRLADGMAVRRADSQALALLAQGLDHVVAVDDAEVGAAMRALHTDT
                     HNLAEGAGAAALAAALQERAALQGQSVGVALTGANVDAALLGEVLRQP"
     gene            5114..5794
                     /locus_tag="PK242_09955"
     CDS             5114..5794
                     /locus_tag="PK242_09955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOZ94383.1"
                     /translation="MGTLYLVRHGQASFGADNYDQLSELGRAQSLRLGQYWRERFGAE
                     LAFDAVLTGTLQRQRQTWQGIVEGAQLSGVPHVEWPGLNEYDSHAVIRAIHPDPLPPP
                     DTPERYRLHFRLLRSGLAAWMRGEVQPAGMPSYADFVRGVTSALEHVRTHYPGQVLLV
                     SSGGPISTAVGQLLSTPAETTIELNMRYRNSAVTELSFTPRRHQLLSYNVLPHLDEAR
                     YRDWVTYA"
     gene            complement(5870..6844)
                     /locus_tag="PK242_09960"
     CDS             complement(5870..6844)
                     /locus_tag="PK242_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HOZ94384.1"
                     /translation="MTTRRHFNASLIAAGAALASPLVRANDDAAIHVLVGFPAGGGSD
                     VIARLLAEKLQAELGRSVVVDNRPGAGGQIAAQQLKAARPDGTTVFLSHDHTISILPQ
                     VVRKPGYAPTEDFVALGGFATFVNGLAVSAATPANTLSEYVEWVRSRQGGKSAIGIPA
                     PASTPEFLVQLIGRKYGLALTAVPYKGSAPMIADMLGNQIPAGTGSVQDFIEYQRAGK
                     LRVIGVLGGKRQAALPDVPTFSEMGFGGLEDTPFYGLWAPRGTPAAFIDRFTRALDKV
                     VALPEVRARLTDLGLTVGYMTPQQLDQRERAYTKVWSRIIADSGFQPQ"
BASE COUNT          904 a         2461 c         2522 g         1067 t
ORIGIN      
        1 gcaacctgat cggctttccg ctgctggtgg cgctggcgct gcgcagcgtg acggcggtgc
       61 acggcgcggt ggttctggcg gtgctgccgc tggcgacggc ggccgctgcg gcgctgctgc
      121 tggggcagcg cgagcggctg ggcttctggc tgtgcgccct ggtgggtgct gcgctggtgg
      181 tgctgtacgc ctggctgcgc gcgcaggcgg ccggcggctt tggctttgcg gcggccgatc
      241 tgctgctgct gggcgccgtg ctcgccgccg ccataggcta cgtggccggt gcccagctga
      301 cgccgcagct cggcgccgag cgggtgatct gctggatgtg tgtgctgggc ctgccgctgt
      361 cgctgccgct ggtggcgtgg tggtggcccg aggcacagac catggcgcag gtgcgcgcgc
      421 cggcctgggc cggcctggtc tacgtgggcg cggtgtccat gtggtcgggc ttcttcgcct
      481 ggtttcgcgg cctcgactgg ggcggcgcgc tgcgcgtcag ccaggtgctg ctgctgcaac
      541 cctttctgac catgctgttc gccgtgccgt tgctgggcga gcggctggat gcgctttcgc
      601 ttgccttcgc ggcggcggtg gtgctcaccg tgttcgtcgg ccggcgcctg tcgcggccca
      661 cccaggcggg cgcgcccctg aacgggcgcg agccgcagac gctttcacca cccaaggggc
      721 aacaaccatg acccagtcac catggcaact ggcggcgcgc gccgaaacca tgaacccgtc
      781 ggcgatccgc gaaattctga agatcaccga gcgccccggc atcctcaact tcgccggcgg
      841 cctgccggca cccgagacct tcccggtgga tgccatgcgc gccgcctgcg ccgcggtgct
      901 ggccgaaggc agcgccgtgg cgcgcccgtc gctgcaatac gccgccagcg aaggcctgcc
      961 cgagctgcgc cagtgggtgg cggccgagct gggccgccag ggcgcgcgcg tggtgcccga
     1021 gcaggtgctg atcaccaacg gcagccagca gggtctggac ctggtggcca aggtgctgct
     1081 ggacgccggc agccgcgtgc tggtggagac accgacctac ctgggggcgt tgcaggcgtt
     1141 caccccgatg cagccgcggt tcgacagcgt ggacagcgac gatggcggtg tcaccgcatc
     1201 gggtctgcgc gagcagctac aaaaacggga gcgaccgcgg ttcgtctacc tgttaccgaa
     1261 cttccagaac ccgagcgggc gcacgcagga cgaggcgcgg cgccagcagg tgatcgcggt
     1321 ctgccgcgag cacgcgctgc ccatcgtcga agacaacccc tacggcgagc tgtggtacga
     1381 ccagccgccg ccggcgccgc tgctggcgcg ctggcccgaa ggggtgatct acctgggctc
     1441 gttttccaag atcctcgcgc ccggcctgcg tctgggctac gtggtggcgc cgccttcgct
     1501 ctaccccaag ttgctgcagg ccaagcaggc ggccgacctg cacacggcgg gtttcaacca
     1561 gcgcgtggtg gccgaggtga tccgggacgg ttttctggat cggcatgtgc ccaccatccg
     1621 ggcccgctac aagacgcagc gcgacgccat gctggcggcg ctggcgcatc acttggatgg
     1681 tctgggtgcc gtctggacac agcccgccgg cggcatgttc atctgggtac gtctgcccga
     1741 aggcctgaac gcccagacgt tgctgccggc cgccgtcgag gccggcatgg ccttcgtgcc
     1801 gggcgcgccg ttctttgccg gccagtccga cgtgcgcacg ctgcgcctgt ccttcgtcac
     1861 ctcgacgccg gcgcagatcg agcgcggcat ggcggcgctg gccagcgtcg tgcgcgctgc
     1921 catgtcaggc accggatctg gctctggcgc atgatggaca agcgcaagca gctatcgata
     1981 gcgtagcaat cgaggttctc catggcacgt cccttcgcgc agatcgacgt tttcaccgcc
     2041 cgtcccggct ggggcaatcc ggtggcggtg gtgctcgacg gccaggggct ggacgacgcc
     2101 gccatgcagc gctttgcgcg ctggaccaac ctgtccgaaa ccaccttcct gctgccgccg
     2161 acggcggccg gcgccgacta cgcgctgcgc atcttcaccc ccggcggcga gctgcccttt
     2221 gccggccacc ccacgctggg cagctgccat tcctggctgg cggcgggcgg caggccgggc
     2281 acgcccgggc gcatcgtgca gcagtgcgcc cagggcctgg tcgagctgcg ccagcacggc
     2341 cagcgcctgg cctttgccgc gcctccgctg cggcgcggcg acgtcccgcc ggcgctgctg
     2401 acgcgcgtgg tgcaggcgct gggcctggcg ccggcccagg tgcggcgcgc ccagtggctg
     2461 gacaacgggc cgcgctggct gggtctgctg ctggacggcg ccgacaccgt gctggcgctc
     2521 gagccggacc acgccgccct cgcagccttg gggcagaaag tcggcgttgc cggcgtccat
     2581 ggcacgccga cagctatacc gttgatagcg cgatcgagcc gtgaagcgcg tgccttcagg
     2641 cccgacgacg acgccgcccc gcggctggag gtgcgcgcct ttgccgcgcc catcggcgtg
     2701 ccggaagacc cggtcaccgg cagcctcaac gccagcctgg cgcagtggct gatcgacgcc
     2761 ggcgagctgc ccgcgcgcta tgtggcggcg cagggcgcgg cgcttgggcg cgccgggcgc
     2821 gtgcacgtgc agcgcgacgc ccagggccag gtgtgggtgg gcggcgactg cgtggcggtg
     2881 atcggcggcg aggtgcggct gtgagtgcgc ggctggacca tatgatggtg ctggccgaca
     2941 cgctggcgca gggcgtcgcc tggtgcgagg ccacgctggg cgtggtgccc gccccgggcg
     3001 gccagcaccc gctgtttggc acccacaacc ggctgctggc cctgggcggc gcggcctttg
     3061 cgcaggctta ccttgagatc atcgcgatcg atccggcggc gggcgatccg ggccgcacgc
     3121 gctggttcga catggacgac caacgcctgc gccggcaggt gcgcgcgcag ggcccgcagc
     3181 tgatccactg ggtggcgcgc gtgcccgagc tggcgcgcgc cgccgcggcc tgggccgcgc
     3241 tggggctcga gcgtggcgcg gtgctgccgg ccagccgcat gacgccgcgc ggcctgctgg
     3301 agtggcagat caccgtgcgg gaggatggcc agcgcctgct ggacggctgc ctgccgaccc
     3361 tgatccagtg gggcgcggtg catcccgccg acgctctgcc ggccagcggc atcgcgctgg
     3421 aatcgctcag cctgcgccac ccgcaggccg cgctgctgcg cgccgcgctg gatgccgtgg
     3481 gcctggcggg cgtggccgtc gaacccgggc ccgccgccct gcaggcgcgc ctggccacgc
     3541 cgcgcggcgc cgtcaccctg gctggttttt catagtcaaa agtggccctg gcgcgctcca
     3601 gtcaagcgcg agcagctacc aataagagag taattcatgt cctccgaacc gacgctcccc
     3661 agccttgccg agatcgaagc tgcggcccag gtggtgtacc gcgccatgcc gcccacgccg
     3721 cagtaccgct gggccctgtt gtccgagcgc ctgggcgcgg actgctgggt caagcacgag
     3781 aaccacggcc cggtgggcgc cttcaagctg cgcggcggcc tgacgttttt cgatgccctg
     3841 gcgcggcgcg gccagctgcc gcgcgaggtc atcagcgcca cccgcggcaa ccacggccag
     3901 agcatcggct gggcggcccg cgcgcacggc gtgcactgca ccatcgtcgt gccgcgcggc
     3961 aactcgctgg agaaaaacgc cgccatgcgc gcgctggggg tcgaactgat cgagcagggc
     4021 gaggacttca ccgccgccgc cgagcacgcg cgccggctgg ccgatgagcg cggcgccctc
     4081 ctggtgccca gctttcaccg cgatctggtg ctgggcgtgg ccacctactg gtgggaattc
     4141 ctgcgcgccg tgccccggtt ggacgtggcc tacgtgccca tcggcctggg ctcgggcgcc
     4201 tgcgcggcgc tggcggccag gcgcgcgctg ggccacccgg cgcgcatcgt cggcgtggtc
     4261 agccgcctgg cgccgaccta tgccctttcg ctggcggcag ggcgggtggt cgaggcgcca
     4321 gccacaacgc gcctggccga tggcatggcg gtgcgccggg ccgactccca ggcgctggcg
     4381 ctgctggccc agggcctgga tcacgtggtg gcggtggacg acgccgaggt cggcgccgcc
     4441 atgcgggcgc tgcacaccga cacccacaac ctggccgaag gcgcgggcgc cgccgcgctg
     4501 gccgccgccc tgcaggagcg cgccgccctg caaggccaga gcgtgggcgt ggcgctcacc
     4561 ggcgccaacg tcgacgccgc cctgctcggc gaggtgctgc gccagccatg accctaggag
     4621 ggtgtcggac ttggagatcg ccggctgcaa atcgaccgca gcggcccatt tttggcttcg
     4681 ccgctcttcg agagcaccgg ctttttgccc aatagcttag ctattgggct gcaaatccgg
     4741 caaaaactgg tctcgctggg gccgattttg gctgcgccgc tcttcgagaa cgctttcggc
     4801 gctccaagcc cgacagcctc ccagaaaccg cagttgaccg cttgcaaccc ttgggaatgc
     4861 cgcatgaact ctgaccttgc accacttggg agccttcacc ttctgggtga aggcgctatg
     4921 cacccgccag caccgctgct gggcgcgcca tgcggcgttg ctcctccttg cgcacgggag
     4981 tgcgcggcgt cgtcacgtct tgcctggcac accgatcacg gcactggcag gcgcatccaa
     5041 ctgccgtttc taggatgacc ggcgctgtcg ccggtttgac gccggcgcgc cgccgggcgg
     5101 gcaagaatgc gccatgggaa ccctttacct cgtgcgccac gggcaggcca gcttcggcgc
     5161 cgacaactac gaccagctga gcgagctcgg tcgcgcccag tcgctgcggc tgggtcagta
     5221 ctggcgcgag cgctttggcg ccgagctggc gttcgacgcc gtgctcaccg gcaccctgca
     5281 gcgccagcgg cagacctggc agggcattgt cgaaggcgcg cagctcagcg gcgtgcccca
     5341 cgtcgagtgg ccgggactga acgagtacga cagccacgcg gtgatccgcg ccatccaccc
     5401 cgacccgctg ccgccgcccg acacgcccga gcgctaccgt ctgcactttc gcctgctgcg
     5461 cagcggcctg gcggcctgga tgcgcggcga ggtgcagccg gccggcatgc ccagctacgc
     5521 ggatttcgtg cgcggcgtca ccagcgcgct ggagcatgtg cgcacccact acccgggcca
     5581 ggtgctgctg gtgtccagcg gcgggccgat cagcacggcg gtggggcagc tgctgtccac
     5641 cccggccgaa accaccatcg agctgaacat gcgctaccgc aactcggccg tgaccgagct
     5701 gagcttcacg ccgcggcgcc accagctgct cagctacaac gtgctgccgc acctggacga
     5761 ggcccgctac cgcgactggg tgacctacgc ctgagacccc aagagctact gaaaatgtag
     5821 ctgccggcgc ttgatgtaag cggttttcag gccttattgg tccaaaagat cactggggct
     5881 gaaagccgct gtcggcgatg atgcgcgacc acaccttggt gtaggcgcgt tcgcgctggt
     5941 cgagctgctg cggcgtcatg tagcccaccg tcaggcccag gtcggtcagg cgcgcgcgca
     6001 cctcgggcag cgccaccacc ttgtcgagcg cgcgcgtgaa gcggtcgatg aaagcggccg
     6061 gcgtgccgcg cggcgcccac aggccgtaga acggcgtgtc ctccagcccg ccgaagccca
     6121 tttcgctgaa ggtgggcacg tcgggcagcg ccgcctggcg cttgccgccc agcacgccga
     6181 tcacgcgcag cttgccggcg cgctggtatt cgatgaagtc ctgcaccgag ccggtgccgg
     6241 ccgggatctg gttgcccagc atgtcggcga tcatcggcgc gctgcccttg tagggcacgg
     6301 cggtcagcgc caggccgtat ttcctgccga tcagctgcac cagaaattcc ggcgtcgagg
     6361 ccggcgccgg gatgccgatg gcgctcttgc cgccctgcct gctgcgcacc cattcgacgt
     6421 actcggacag cgtgttggcc ggcgtcgccg ccgacaccgc cagcccgttg acgaaggtgg
     6481 caaagccgcc cagcgccacg aagtcctcgg tcggtgcgta gccgggcttg cgcaccacct
     6541 gcggcaggat cgagatggtg tggtcgtgcg acaggaacac cgtggtgccg tcgggccgcg
     6601 ccgccttgag ctgctgcgcc gcgatctggc cgccggcgcc ggggcggttg tccaccacca
     6661 cgctgcggcc cagttcggcc tgcagtttct cggccagcag gcgggcgatg acgtcggagc
     6721 cgccgccggc cggaaaaccc accagcacgt ggatggctgc atcgtcgttg gcccgcacca
     6781 ggggcgaggc cagcgcggca ccggcggcga tcagggaggc gttgaagtgg cggcgcgtgg
     6841 tcatgggctg tcgtccgtgg tggttggcaa ggttgccgat tctaggaggc tgtgctggcg
     6901 cagcgtgtcg gccggcaggc ggtcgatctc ctgcagcagc gcggccaggg cgcg
//
LOCUS       DAOOOG010000135         6938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_12921_length_6938_cov_23.451257, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000135 DAOOOG010000000
VERSION     DAOOOG010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12921_length_6938_cov_23.451257"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="PK242_09965"
     CDS             <1..815
                     /locus_tag="PK242_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ94385.1"
                     /translation="AKALQALVGGSADVVSGAYEHTINMQAKNQPIMSFVLQGRAPQI
                     VLAVSTRNMPGYKSIADLKGKKIGVTAPGSSTAMMASYVLAKAGLKASDVSFIGVGAS
                     SGAIAAVKSGQVDAIANLDPVITMLQRDNLIKVVADTRTLKDTQSVYGGPMPAACLYT
                     PVKFVQDNPGTTQALANAMVRALRWLQKAGPSDIVKTVPDSYLLGDRALYLAAWERVR
                     EAISPDGLMPEAGPATALRMLQTFEDSLKGKPIDLGKTYTNNFARKAAAKYA"
     gene            916..1761
                     /locus_tag="PK242_09970"
     CDS             916..1761
                     /locus_tag="PK242_09970"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ94386.1"
                     /translation="MTPSSAAALDLQDITCIFTSRDAPGQRYTAVQGASLAVAPGEFV
                     SVVGPTGCGKSTLLNVGAGLLAPSGGQVQVFGQPLAGLNRRAGYMFQTESLMPWKTAL
                     DNVVAGLVFRGTDATEADRLGRAWLARVGLAGFEDRYPHQLSGGMRKRVAMAQTLILD
                     PDIILMDEPFSALDVQTRQLMENELLDLWAAPSASGSAQGRAVLFITHDLDEAIALSD
                     RVVVLSAGPATRPIGEFAIDLPRPRNVTEIRNAPRFVELHAQIWAVLRDEVLKGYDQQ
                     KRKAA"
     gene            1758..2537
                     /locus_tag="PK242_09975"
     CDS             1758..2537
                     /locus_tag="PK242_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ94387.1"
                     /translation="MNRVVLRAWQFGLLLVLLLLWHFASQGSDTVAFFFGKPLEVAQR
                     LWRWFVTDADIYRHLWVTLLETLLAFVIGTLAGLAVGLWLALSPFASAVLDPYLKAAN
                     AMPRVILAPIFAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVREVSPVVLANARMLGA
                     NARQLMRHVYVPSAMSWVFSSLHTSVGLAFVGAVVGEYLGSASGVGYLILQAEGSFDI
                     NTVFAGIVLLTVFALVLDGLVGLAERHLMKWQPSSGQTEKL"
     gene            complement(2639..3013)
                     /locus_tag="PK242_09980"
     CDS             complement(2639..3013)
                     /locus_tag="PK242_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94388.1"
                     /translation="MECFTHPGSSSVGICKTCGKAVCRTCAIDLGFAVACTQACATEA
                     AAIHEMNQRGKKIYGIGVARPKIPSGVIMWLLFGALFSGFGAFAWFKARDLGWFLLLF
                     GAASFFVAWLGYRRAKDVGLQC"
     gene            complement(3245..3628)
                     /locus_tag="PK242_09985"
     CDS             complement(3245..3628)
                     /locus_tag="PK242_09985"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOZ94389.1"
                     /translation="MGLLGLLLSVAIAGRQLALLAVAQSAPGTVIEMVRMASGRLSAA
                     DAYAPRVRFVLDAVEHEFVSPVGSYPPAFQIGDTVRVLYDPANPRRAMVDSSGQLWAP
                     VAVCAVLGLLLGGLGAWLLRKSRVA"
     gene            complement(3871..4320)
                     /locus_tag="PK242_09990"
     CDS             complement(3871..4320)
                     /locus_tag="PK242_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ94390.1"
                     /translation="MKILLPVDGTELSLHETRFALRLVREGLRASFVLANVQEPASFY
                     EIVTSQNPGLIESAAQEAGEDLMAPSARLLREAGVSFETAVITGDPAHAMLELIELHG
                     CDMVVMGARALGPIRRMLEGGSTSRRLLDDAPVPVLLVKPPPQPQQD"
     gene            complement(4394..6382)
                     /locus_tag="PK242_09995"
     CDS             complement(4394..6382)
                     /locus_tag="PK242_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOZ94391.1"
                     /translation="MPQPHQIELLAPARDADIGIEAIQHGADAVYIGGPGFGARAGAG
                     NPVADIERLARHAHRFDARIFVTLNTILRDDELEPARRLAWQLYDAGADALIIQDMGL
                     LQLDLPPIQLHASTQTDIRTPAKARFLQDAGLSQIVLARELDLTQIAAICDVLEPARC
                     GIEFFIHGALCVAYSGQCYISHAHTGRSANRGDCSQACRLPYQVVDAQGRFVAHDAHV
                     LSMKDNNQSLNLGPLIDAGVRSFKIEGRYKDMGYVKNITAHYRRLFDDILEQRPELAR
                     ASSGRCRFGFTPDPLQNFNREFTDYFVHGRQIDVGAFDTPKNPGQPIGHVTRVAGDHF
                     ELQTDDPALTLANGDGLCFWDPLKTLVGAQANRVEALGDGRWRVFPKDPMAQLRELRR
                     GTPVHRNRSMDWVRALERKSAERRIDVWLTLSETADGLSLSAIDEDGHSGAAQVALAW
                     AAPRQPAAAEHALREALARLGETLFAPLGIALALPRPWFVPPSVANALRRDAIAALEA
                     ARAAAWQRLPRAAPVEPPTPYPEDTLSYLANVFNHKARDFYARHGVRVIDAAYESHQE
                     PGEVSLMITKHCVRYSLSLCPKQAKGVVGVKGTIRAEPLQLINGKERLTLRFDCKPCE
                     MHVVGRIKPGVVQRAEREAAEHALRFYRAPPRGARSFE"
     gene            complement(6417..>6938)
                     /locus_tag="PK242_10000"
     CDS             complement(6417..>6938)
                     /locus_tag="PK242_10000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ94392.1"
                     /translation="AEQMRRLGEFVASYLAHLRLPVRRVLDMGCGIGLWRDHVARLFP
                     GASYLGVEYSEHLCQRYGWSRGSVVDWRADEPFDLVICQGVLAYLNPSDLRRALANLD
                     RLCRGALYLEAVTLEDYERDAIDLELSDPRLYRHRAALYRRGLTPHFRAAGGGLWLSR
                     RAPVPLLALEAPD"
BASE COUNT         1027 a         2317 c         2504 g         1090 t
ORIGIN      
        1 gcgccaaggc cttgcaggcg ctggtgggcg gcagcgccga cgtggtgtcg ggcgcttacg
       61 agcacaccat caacatgcag gccaagaacc agcccatcat gtccttcgtg ctgcagggcc
      121 gggcgccgca gatcgtgctg gcggtgtcga ccaggaacat gccgggctac aagtcgattg
      181 ccgacctgaa gggcaagaaa atcggcgtca cggcgcccgg ttcgtccacc gccatgatgg
      241 ccagctacgt gctggccaag gcggggctga aggcgagcga cgtgtccttc atcggcgtgg
      301 gcgcgtccag cggcgccatc gccgccgtca agtcgggcca ggtcgatgcc atcgccaacc
      361 tcgacccggt gatcaccatg ctgcagcgcg acaacctgat caaggtggtg gccgacacgc
      421 gcacgctcaa ggacacccag tcggtctacg gcggcccgat gccggcggcc tgcctgtaca
      481 cgccggtgaa gttcgtgcag gacaaccccg gcaccacgca ggcgctggcc aacgccatgg
      541 tgcgcgcgct gcgctggctg cagaaggccg gcccgtcgga catcgtcaag accgtgcccg
      601 actcgtacct gctgggcgat cgcgcgctgt acctggcggc ctgggagcgc gtgcgcgaag
      661 ccatctcgcc cgacggcctg atgcccgagg ccggcccggc caccgcgctg cgcatgctgc
      721 aaaccttcga ggacagcctg aagggcaagc ccatcgacct gggcaagacc tacaccaaca
      781 acttcgcccg caaggcggcg gccaagtacg cctgaccacg ccggcggccg gcacggcccc
      841 ggggtggggc cgagttacta tcaatttcgt agctgccggc gcttgccctg cctgagccag
      901 cgccttgttt tgcttatgac accttcttcc gccgccgcgc tggacctgca ggacatcacc
      961 tgcatcttca ccagccgcga cgcccccggt cagcgctaca cggcggtgca gggcgccagc
     1021 ctggcggtgg cgccgggtga attcgtgtcg gtggtggggc ccactggctg cggcaagagc
     1081 acgttgctga acgtgggcgc cgggctgctg gcgccatccg gtgggcaggt gcaggtgttc
     1141 ggccagccgc tggcggggct gaaccggcgt gccggctaca tgttccagac cgagtcgctg
     1201 atgccctgga agacggcgct ggacaacgtg gtggccgggc tggtgtttcg cggcaccgac
     1261 gccaccgagg ccgaccgcct gggccgcgcc tggctggcgc gcgtgggcct ggccggcttc
     1321 gaggaccgct acccgcacca gttgtcgggc ggcatgcgca agcgcgtggc gatggcgcag
     1381 acgctgatcc tcgaccccga catcatcctg atggacgagc cgttttcggc gctcgacgtg
     1441 cagacccgcc agctgatgga aaacgagttg ctcgatctgt gggccgcgcc ctcggccagc
     1501 ggcagcgcgc aggggcgggc ggtgctgttc atcacgcacg acctggacga ggcgatcgcc
     1561 ctgtccgacc gcgtggtggt gctgtcggcc ggcccggcca cgcggccgat cggcgaattc
     1621 gccatcgacc tgccgcgccc gcgcaacgtg accgaaatcc gcaacgcgcc gcgcttcgtc
     1681 gagctgcacg cgcagatctg ggccgtgctg cgcgacgaag tgctcaaggg ctacgaccag
     1741 caaaaaagga aggccgcatg aaccgcgtgg tgctgcgcgc ctggcagttc gggctgctgc
     1801 tggtgctgct gctgctgtgg cactttgcct cgcagggctc ggacaccgtg gccttcttct
     1861 tcggcaagcc gctggaagtg gcgcagcgcc tgtggcgctg gttcgtgacc gacgccgaca
     1921 tctaccgcca cctgtgggtg acgctgctgg aaacactgct ggccttcgtg atcggcaccc
     1981 tggccgggct ggcggtgggc ctgtggctgg cgctgtcgcc gttcgcgtcg gcggtgctgg
     2041 acccgtacct gaaggccgcc aacgccatgc cgcgcgtgat cctggcgccg atcttcgcca
     2101 tgtggttcgg gctgggcatc tggtccaagg tggcgctggc ggtgacgctg gtgttcttca
     2161 tcgtgttctt caacgtctac cagggcgtgc gcgaggtcag cccggtggtg ctggccaacg
     2221 cgcgcatgct gggcgccaac gcgcgccagc tgatgcgcca cgtgtacgtg cccagcgcca
     2281 tgagctgggt gttttcgagc ctgcacacgt cggtgggact ggccttcgtg ggggcggtgg
     2341 tgggcgaata cctgggctcg gccagcggcg tgggttacct gatcctgcag gccgaaggtt
     2401 cgttcgacat caacaccgtg ttcgccggca tcgtgctgct gacggtgttt gccctggtgc
     2461 tcgacggcct ggtcggtctg gccgagcggc acctgatgaa gtggcagccg agcagcgggc
     2521 agaccgaaaa gctgtgagcg ctgcggcttg ctgccctggc ccctctgggc agagggcggg
     2581 gtgaggggtg gcggtggatg ccggagcctc tgctggccgg gatgtgcgcc cggtggcgct
     2641 agcactggag cccgacatcc ttggcgcggc ggtagcccag ccatgccaca aagaaggaag
     2701 cggcaccaaa cagcagaagg aaccacccga ggtctcgcgc tttgaaccac gcaaaggcac
     2761 cgaagccgct gaacagggcg ccaaacaaca gccacatgat caccccgctg gggatcttcg
     2821 gacgggcaac cccgatgccg tagatcttct tgccgcgctg gttcatttcg tggatggcgg
     2881 ctgcctcggt ggcgcatgcc tgggtacagg cgacggcgaa ccccagatcg atggcgcagg
     2941 tgcggcacac cgccttgccg caggtcttgc atattcccac tgaactggaa cccggatggg
     3001 taaagcattc cattggaaat cactcctgac gcccgggtgg gatcgcacgg caccggagca
     3061 ccgaagcgcc cgaatccgca gcgggccaaa agcggacatg ttagcggtcg gcgccaccct
     3121 atgggttcgg cctattgatc cggtcctttg aagtctcaaa atccgttcgg gctgagcttg
     3181 tcgaagccct tcgacaggct ctgggtgaac ggttccaatg tcaaggttcc gaagccaaag
     3241 agactcaggc tacgcgcgac ttgcgcagca gccaggctcc gaggccaccg aggagcaagc
     3301 ccagcaccgc gcacaccgcc accggcgccc agagttgacc ggagctgtcg accatggccc
     3361 gccgcgggtt ggccgggtca tacagaacgc gaaccgtgtc gccgatctgg aaggcgggag
     3421 ggtaactgcc gacgggggac acgaactcgt gctcgacggc atcgaggacg aagcgcacgc
     3481 gcggggcgta ggcatccgct gcagagaggc ggcccgaagc catgcgcacc atctcgatca
     3541 ccgttcccgg cgcgctctgg gcgacagcca gcagggccag ttgccgcccg gcgatggcca
     3601 cgctcagaag cagcccgagc agtcccaccg ccagcaggaa ccggccccat cggcgtcgac
     3661 gccgtgcctc ttctggtagg gggctagtcc gagccgtcat ggatggtcaa catggggtcg
     3721 agtgccgatg gtggcgcaac ggcaagctgc ttgcctgact ctgtcgaccg cctgcggtcg
     3781 cctgggtggc tggctgatcg actgcggcgt tcgagtcgag cccgcgagcc ccgcagcgca
     3841 gcgccggcgc gctccaccgg tcggtcgcgg tcagtcctgc tgcggttggg gcggcggttt
     3901 gaccagcagc acgggcaccg gcgcgtcgtc cagcagccgg cgcgaggtgg agccgccttc
     3961 gagcatgcgg cggatcgggc ccagggcgcg cgcgcccatc accaccatgt cgcagccgtg
     4021 cagctcgatc agctccagca tggcgtgcgc cggatcgccg gtgatgaccg ccgtctcgaa
     4081 gctcacgccg gcttcgcgca gcaggcgggc ggacggcgcc atcaggtctt cgccggcttc
     4141 ctgcgcggcg ctttcgatca ggcccgggtt ctgcgaggtc acgatttcgt agaacgaggc
     4201 cggctcctgc acgttggcca gcacgaagct ggcgcgcagg ccttcgcgca ccagacgcag
     4261 cgcaaagcgg gtttcatgca gggacagctc ggtgccgtcg acggggagca ggatcttcat
     4321 ggttgggtcc tcgatcagat gctataaaca agatagctgc tggcgcttgc tggtagcgcg
     4381 ccagcggccc atttcattcg aatgaccggg ccccccgcgg aggcgcgcgg taaaagcgca
     4441 gggcgtgttc ggcggcttcg cgctcggcgc gctgcaccac gccgggcttg atcctgccga
     4501 ccacgtgcat ctcgcacggc ttgcagtcga agcgcagcgt caggcgctcc ttgccgttga
     4561 tgagctgcag cggctcggcc cggatggtgc ccttgacgcc caccacgccc ttggcctgct
     4621 tggggcacag gctgaggctg tagcgcacgc agtgcttggt gatcatcagg ctgacttcgc
     4681 ccggttcctg gtggctttca taggcggcgt cgatgacgcg cacgccgtgg cgcgcgtaga
     4741 agtcgcgcgc cttgtggttg aacacgttgg ccaggtagct gagcgtgtct tcggggtagg
     4801 gcgtgggcgg ctcgacgggc gcggcgcgcg gcaggcgctg ccaggcggcg gcgcgcgcgg
     4861 cttcgagtgc ggcgatggcg tcgcgccgca gcgcgttggc cacgctgggc ggcacgaacc
     4921 aggggcgcgg cagcgcgagc gcgatgccca gcggcgcgaa cagggtttcg cccagccgcg
     4981 ccagcgcttc gcgcagcgcg tgctcggccg ccgccggctg gcgcggcgcg gcccaggcca
     5041 gcgcgacctg ggcggcgcca ctgtggccgt cttcatcgat ggcgctcagg ctcagaccgt
     5101 cggccgtttc gctcagggtc agccagacgt cgatgcggcg ctcggccgac ttgcgctcca
     5161 gcgcgcgcac ccagtccatg ctgcggttgc ggtgcaccgg cgtgccgcgg cgcagctcgc
     5221 gcagctgggc catcgggtcc ttggggaaca cgcgccagcg gccgtcgccc agcgcctcga
     5281 cgcggttggc ctgcgcgccc accagggtct tcagcgggtc ccagaagcac aggccgtcgc
     5341 cattggccag ggtcagcgcc gggtcgtcgg tctgcagctc gaagtgatcg cccgccacgc
     5401 gcgtgacgtg gccgatcggc tggccggggt tcttgggcgt gtcgaaggcg ccgacgtcga
     5461 tctggcggcc gtgcacgaag tagtcggtga actcgcgatt gaagttctgc agcgggtcgg
     5521 gtgtgaagcc gaaccggcag cggccgctgc tggcgcgcgc cagctcgggg cgctgctcca
     5581 ggatgtcgtc gaacaggcgg cgatagtggg cggtgatgtt cttcacgtag cccatgtcct
     5641 tgtagcgccc ctcgatcttg aagctgcgca cgccggcgtc gatcagcggg cccaggttga
     5701 gcgactggtt gttgtccttc atgctcagca cgtgggcgtc gtgggccacg aagcggccct
     5761 gcgcgtccac cacctggtag ggcaggcggc aggcctgcga gcagtcgccg cggttggcgc
     5821 tgcggccggt gtgggcgtgg ctgatgtagc actggccgct gtaggccacg cacagcgcgc
     5881 cgtggatgaa gaactcgatg ccgcagcgcg ccggctccag cacgtcgcag atggcggcga
     5941 tctgggtcag atcgagctcg cgcgccagca cgatctggct cagccccgca tcctgcagaa
     6001 agcgcgcctt ggccggcgtg cggatgtcgg tctgggtgct ggcgtgcagc tggatcggcg
     6061 gcaggtcgag ctgcagcagg cccatgtcct ggatgatcag tgcgtcggcg ccggcgtcgt
     6121 acagctgcca ggccaggcgg cgcgccggct ccagctcgtc gtcgcgcaag atggtgttga
     6181 gggtgacgaa gatgcgggcg tcgaagcggt gcgcgtggcg cgccaggcgc tcgatgtcgg
     6241 ccaccgggtt gccggcgccg gcgcgcgcgc caaagcccgg cccgccgatg tagacggcgt
     6301 cggcgccgtg ctggatggcc tcgatgccga tgtcggcgtc gcgcgcgggg gccagcagtt
     6361 cgatctggtg cggctggggc atggagcgga ttatccccag cgccgcgggc gccggttcag
     6421 tcgggcgctt ccagcgccag cagcggcacc ggcgcgcgcc ggctcagcca caggccgccg
     6481 ccggcggcgc ggaaatgggg cgtcaggccg cgccggtaca gcgccgcgcg gtggcggtac
     6541 aggcgcgggt cgctcagctc caggtcgatg gcgtcgcgtt cgtagtcttc cagcgtcacc
     6601 gcttccaggt acagcgcgcc gcggcacagc cggtccaggt tggccagcgc gcgccgcagg
     6661 tcgctcgggt tcaggtaggc cagcacgccc tggcagatca ccaggtcgaa cggctcgtcg
     6721 gcgcgccagt cgaccaccga gccgcggctc cagccgtagc gctggcacag gtgctcgctg
     6781 tattccacgc ccaggtagct ggcgccgggg aacagccgcg ccacgtggtc gcgccacagg
     6841 ccgatgccgc agcccatgtc gagcacgcgc cgcaccggca ggcgcaggtg cgccaggtag
     6901 ctggcgacga actcgcccag ccggcgcatc tgctcggc
//
LOCUS       DAOOOG010000136         6912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_12987_length_6912_cov_27.071022, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000136 DAOOOG010000000
VERSION     DAOOOG010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12987_length_6912_cov_27.071022"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PK242_10005"
     CDS             <1..401
                     /locus_tag="PK242_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOZ94393.1"
                     /translation="ILHSFAGRIGLLLEKLLAPVGFNWQIAIALVPGMAAREVAVGAL
                     GTVYALSASGDDVAAQLQPLIAHGWSLATALALLAWYVYAPQCVSTLVAVKRETNGWR
                     WPLVMLVQLFGLAWLAAFITYRTALAMGAG"
     gene            401..595
                     /locus_tag="PK242_10010"
     CDS             401..595
                     /locus_tag="PK242_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94394.1"
                     /translation="MQALAVALIVTLAALYVLWRFLPARWRNLAAKRLGLGAGAGQAG
                     HCHACGDCGACATPAADSDK"
     gene            complement(660..989)
                     /locus_tag="PK242_10015"
     CDS             complement(660..989)
                     /locus_tag="PK242_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOZ94395.1"
                     /translation="MKNRAANLSPCERCGACCATFRVDFSIMETQAEGGAVPDGLALP
                     VAARTCRLRGTDHLPPRCAALSGRVGERVACSIYEWRPSPCREFSEGSDACAQARRRH
                     RLEPLAV"
     gene            1186..1548
                     /locus_tag="PK242_10020"
     CDS             1186..1548
                     /locus_tag="PK242_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOZ94396.1"
                     /translation="MRTDMFQQVLEELKGSSADVEASALISTDGLMIASALPAGMDED
                     RVGAMSAALLSLGDRAVRELARGTLERVLLQGERGYVIMSSSGTEAVLTVLAKQNAKL
                     GLLFLDIKRAAEALSKLI"
     gene            1562..2053
                     /locus_tag="PK242_10025"
     CDS             1562..2053
                     /locus_tag="PK242_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOZ94397.1"
                     /translation="MAFPPMQPAADADQARQHTYHDGTSRTVQVSEREIPYPEGRLII
                     SRTDLNGVITHANDAFIELSGYTREELIGQPHAVLRHPDMPRATFKDLWDTMKAGKKW
                     HGYVKNLRKDGSHYWVYATAVPNIRNGQTVGYTSVRRKPSRKKIEELSAVYKEWKAAE
                     GST"
     gene            2053..2802
                     /locus_tag="PK242_10030"
     CDS             2053..2802
                     /locus_tag="PK242_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOZ94398.1"
                     /translation="MTLNLLIAPDFAPERFAGWHMLNTLLQKRSGLQLHLLTPASAAE
                     QAQLIAEGKVDAVYANPFDAASMIRDAGYQAIARPVGKSDEMVIATAAGSAANTVEDL
                     QPGCKIALTDNKDVKLIGLRLLEPADLTEADIQWQMVDTYQAAARMAIKGEVDASFFL
                     AEAYHSLSKLTKSQLKALMESKLADITHVLLTSAKVGADAERVKEALVGLTGTPDGQP
                     VLDALGIAGGFEPMTQEDAEFMIDLMDTLLD"
     gene            2802..3389
                     /locus_tag="PK242_10035"
     CDS             2802..3389
                     /locus_tag="PK242_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94399.1"
                     /translation="MRNAQHGLSARQQFRQAGRRVLNAPADLALHEQRVNAALGLEGA
                     EPLQGALADWLHGCAPDAARFQALLARADIQPRLAPFVLQGLQAQAASGRKLPAASVW
                     ATRWSVLVAPSLDVPRRALLCSVDDSRRIAAEALPALLAGDAQAEQDFLAHCEGAGDS
                     LGFMLVRRALTREGRALSPQWEAVSLALQNGVIQA"
     gene            3386..4912
                     /locus_tag="PK242_10040"
     CDS             3386..4912
                     /locus_tag="PK242_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94400.1"
                     /translation="MKNILLMGLTDHEAAAVEIMIGMNWRDHRCITLKRELPLAIPEQ
                     HAAARSCEACVLDLFGLGMRKYSPANATRLMEVMAGRPAVLLTGGDGGGWLEAKLPLA
                     KGQHIGWVGMPYTSAGLRDAIKKVKEAAERMVPPAAAAVAAAPAAMPGRARPVVSLDV
                     EPALPAAVAEAEPAAVPAWRRAMELADQLKRKAAPVVSARPAARAAVTEAVLANPPAR
                     RPAAPVAPPPTPAPEPAPMRARASAPMPLPKAQASSVSLAPPATRMATDAVGLGKGAL
                     AALLQVFPLLRDQPLIGLTEKIIGHDGPMLLRIGTDAAFVTHTRAGWLASGLRVSALP
                     KLLRTPRLVESVRAEPLDPDHVEETVRQRFDGKFHRAQKPLDVITWELVSDVMRDMKL
                     QRQGDLTFQLRRFPNFPMLAGVGPLDVQLAAICARMPQSISELLRAFPKHEQDVLRFV
                     VLCVVSGLAKVIPGGPVAAGAVAPPRAAQAQAPVAAAARKPEVAARRGFFKSLMDKLF
                     "
     gene            4925..5470
                     /locus_tag="PK242_10045"
     CDS             4925..5470
                     /locus_tag="PK242_10045"
                     /inference="COORDINATES: protein motif:HMM:NF015018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HOZ94401.1"
                     /translation="MASIYKLVFAGPVGAGKTTAIQSLSDIEVVRTEANASDDVKTLK
                     KTTTVAMDYGLMKLASGDQVRLYGTPGQKRFDFMWDILTENALGLVLMLRGNSPDPVA
                     DLRTYVTEFRDFIDRTSLVVGITHSDQGGWQTRQMVSRELVAMGLPPTAMDTDARSRA
                     HMATLVKALIYGIDPFNNAEE"
     gene            5470..6144
                     /locus_tag="PK242_10050"
     CDS             5470..6144
                     /locus_tag="PK242_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94402.1"
                     /translation="MNETPVSADAPADDPYLVLTPAGALHAYGERVPDETSAILQTLM
                     PRGASLRRSAWLELAPEHRTVLARALYEGWVHEVQRELRAPDVRLDNYLPHAIAGLSG
                     TRTAALASDEGFCLARVGYSEEEAETLCVVAADFFDFAKRQKQRGWTGTGRAVSFHEG
                     IDMLLPTTTLMLFWVDGTGYWLILGGEPLLNNRALVELIWGIRTAGTKFAVRETTSIA
                     SQILAM"
     gene            complement(6326..>6912)
                     /locus_tag="PK242_10055"
     CDS             complement(6326..>6912)
                     /locus_tag="PK242_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOZ94403.1"
                     /translation="AAHSRVPVINGLTNEYHPCQILADIFTYIEHRGPIQGRVVAWIG
                     DGNNMANTWAQAAALLGFELRVSTPRGYEVDTNLAALDGNGGNKDWHNFADPMQACEG
                     ADLVTTDVWTSMGYEAENEARKQAFADWCVDAEMMAAAKPDALFMHCLPAHRGEEVTA
                     DVIDGPQSVVWDEAENRMHVQKALMEYLLLGRLA"
BASE COUNT         1068 a         2358 c         2416 g         1070 t
ORIGIN      
        1 ccattctgca cagctttgcc ggccgcattg gcctgctgct ggaaaagctg ctggcaccgg
       61 tcggcttcaa ctggcagatc gccatcgcgc tggtgcccgg catggcggcg cgcgaggtgg
      121 cggtgggcgc gctgggcacg gtgtatgcgc tgtcggccag cggcgacgac gtggcggcgc
      181 agttgcagcc gctgatcgcc catggctgga gcctggcgac ggcgctggcg ttgctggcct
      241 ggtacgtgta tgcgccgcag tgcgtgtcga cgctggtggc cgtcaagcgc gagaccaacg
      301 gctggcgctg gccgctggtc atgctggtgc agctgttcgg gctggcgtgg ctggcggcgt
      361 tcatcaccta ccgcacggcg ctggcgatgg gggcagggtg atgcaggcgc tggccgtcgc
      421 actgatcgtc acgctggcgg cgctgtacgt gctgtggcgt ttcctgccgg cacgctggcg
      481 caatctggcc gcgaaacgcc tgggcctggg tgcgggcgcc ggccaggcgg gccactgtca
      541 tgcctgcggc gactgcggcg cgtgcgcgac gccagccgcg gattcagata aataatgcct
      601 gtatcccttg ctggataagc gccagaagct attttttgaa tagcaaactg gccgtgcgat
      661 cagaccgcca gcggctccag ccgatgccgc cgccgggcct gcgcacaggc gtcgctgccc
      721 tcgctgaact cgcggcaagg cgagggccgc cattcataga tgctgcaggc cacgcgctcg
      781 cccacgcggc ccgacagcgc cgcgcagcgc ggcggcaggt ggtcggtgcc gcgcaggcgg
      841 caggtgcggg cggcgacggg cagcgccagg ccatcgggca ccgcgccccc ttccgcctgt
      901 gtttccatga tcgagaagtc cacccggaag gtggcgcagc aggcgccgca gcgctcgcag
      961 gggctgagat tcgctgccct gtttttcatg gcaaagctct tgaatgaata cttaggttct
     1021 tagtccccgg gggtatgcac accggtgtga tattcgaccg cgaaataagt ggcgcctgca
     1081 cgggtctgta ttccgggaac tatggtcatc ggtgtggttt tgcattctaa tttgcatgcc
     1141 gcggccactt ggcgaagcgg gctaccaccg acaaaggaag aaattatgcg tactgacatg
     1201 ttccagcagg tgctggaaga gctcaaaggt tcgtctgccg acgttgaggc atcggccctg
     1261 atttccaccg acggtctgat gatcgcctcg gccctgccgg ccggcatgga cgaagaccgc
     1321 gtgggcgcca tgtccgccgc gctgctgtcg ctgggcgacc gcgcagtgcg cgaactggcc
     1381 cgcggcacgc tggagcgcgt gctgctgcag ggcgagcgcg gctacgtgat catgagttcg
     1441 tcgggcaccg aagccgtgct gacggtgctg gccaagcaga acgccaagct gggcctgctg
     1501 ttcctggaca tcaaacgcgc cgccgaggcg ctgtccaagc tgatctgagg agggtttcgc
     1561 catggctttc cccccgatgc agcccgccgc ggatgcggac caggcgcgcc agcacaccta
     1621 ccacgacggc accagccgca cggtgcaggt gagcgagcgc gagattccct accccgaagg
     1681 tcgcctgatc atctcgcgca ccgacctcaa cggcgtcatc acgcacgcca acgacgcctt
     1741 catcgaactc tcgggctaca cccgtgagga actgatcggc cagccgcacg ccgtcctgcg
     1801 ccaccccgac atgccgcgcg ccaccttcaa ggacctgtgg gacaccatga aggccggcaa
     1861 gaagtggcac ggctacgtga agaacctgcg caaggacggc tcgcactact gggtgtacgc
     1921 caccgccgtg cccaacatcc gcaacggcca gaccgtgggc tacacctcgg tgcgccgcaa
     1981 gccgtcacgc aagaagatcg aagaactgtc ggccgtctac aaagagtgga aagctgccga
     2041 agggagcacc tgatgacctt gaatttgctg attgcccccg acttcgcgcc cgagcgcttt
     2101 gccggctggc acatgctgaa cacgctgctg cagaagcgct ccggcctgca actgcacctg
     2161 ctgacgcccg catcggccgc cgaacaggcg cagctgatcg ccgagggcaa ggtggatgcg
     2221 gtgtatgcca acccgttcga cgcggcgtcg atgatccgcg acgccggcta ccaggccatc
     2281 gcacggccgg tgggcaagtc ggacgagatg gtgattgcca cggccgccgg ctcggccgcc
     2341 aacacggtgg aagacctgca gcccggctgc aagatcgcgc tgaccgacaa caaggacgtc
     2401 aagctgatcg gcctgcgcct gctggagccg gccgacctga ccgaggccga catccagtgg
     2461 cagatggtgg acacctacca ggccgccgcg cgcatggcca tcaagggcga ggtcgacgcc
     2521 agcttcttcc tggccgaggc ctaccactcg ctgtcgaagc tgaccaagtc gcaactgaag
     2581 gcgctgatgg aaagcaagct ggccgacatc acgcatgtgc tgctcaccag cgccaaggtc
     2641 ggcgccgacg ccgagcgcgt gaaagaggcg ctggtcggcc tgacgggcac gcccgacggc
     2701 cagccggtgc tggacgcgct gggcatcgcc ggcggcttcg agccgatgac gcaggaagac
     2761 gccgagttca tgatcgacct gatggacacg ctgctcgatt gatgcgcaac gcccagcacg
     2821 gcctgtccgc caggcagcag ttccgccagg caggtcgccg ggtgctgaat gcgcccgccg
     2881 acctggcgct gcacgagcag cgggtgaacg ccgcactggg cctggagggc gccgagccgc
     2941 tgcagggcgc cctggccgac tggctgcatg gctgcgcgcc cgatgcggcg cgctttcagg
     3001 ccctgctggc gcgtgccgac attcagccgc ggctggcgcc gttcgtgctg cagggcctgc
     3061 aggcgcaggc tgccagcggc cgcaagctgc ccgccgccag tgtctgggcc acgcgctgga
     3121 gcgtgctggt cgcgccctcg ctggacgtgc cgcggcgggc gctgctgtgc agcgtcgacg
     3181 attcgcgccg catcgccgcc gaggcgctgc cggcgctgct ggcgggcgac gcgcaggccg
     3241 agcaggactt tctcgcgcac tgcgagggcg ccggcgattc gctgggcttc atgctggtgc
     3301 gccgcgcgct cacacgcgaa ggccgggcac tgtcgccaca atgggaagcc gtgtcgctgg
     3361 ctttgcagaa cggagtgatc caagcatgaa gaacatcctc ctgatgggcc tgaccgatca
     3421 tgaggccgct gccgtcgaga tcatgatcgg catgaactgg cgcgaccacc gttgcatcac
     3481 gctcaagcgc gagctgccgc tggccatccc cgagcagcac gccgccgccc gcagctgcga
     3541 agcctgcgtg ctggatctgt tcggcctggg catgcgcaag tattcgccgg ccaacgccac
     3601 gcgcctgatg gaagtgatgg ccggccggcc ggccgtgctg ctgaccgggg gcgacggcgg
     3661 gggctggctg gaggccaagc tgccgctggc caagggccag cacatcggct gggtcggcat
     3721 gccctacacc agtgccggcc tgcgtgacgc gatcaagaaa gtcaaggaag ctgccgaacg
     3781 catggtgccg ccggcggccg ccgccgtggc cgctgcgccc gccgccatgc cgggcagggc
     3841 gcggccggtg gtgtcgctcg acgtcgagcc cgcgctgccc gcggcggtgg ccgaagccga
     3901 gcccgcggcc gtgccggcct ggcgccgcgc catggaactg gccgaccagc tcaagcgcaa
     3961 ggcggcgccg gtggtcagcg cgcggccggc ggcccgcgct gccgtgaccg aggcggtgct
     4021 ggccaacccg ccggcacgcc gcccggcagc gcccgtcgcg ccgccaccca cgccggctcc
     4081 cgagccggca cccatgcgtg cacgtgcctc ggcgccgatg ccgctgccga aggcgcaagc
     4141 atcttccgtc tccctggcgc cgccggccac gcgcatggcc accgacgcgg tcggcctggg
     4201 caagggtgcg ctggcggcgc tgctgcaggt gtttccgctg ctgcgcgacc agccgctgat
     4261 cggcttgacc gaaaaaatca ttggtcacga cggcccgatg ctgctgcgca tcggcaccga
     4321 tgcggccttc gtcacccaca cgcgcgccgg ctggctggcc tccgggctgc gggtgtcggc
     4381 cctgccgaag ctgctgcgca cgcccaggct ggtggagagc gtccgcgccg aaccgctcga
     4441 ccccgaccac gtcgaggaga cggtgcgcca gcgcttcgac ggcaagttcc accgcgcgca
     4501 gaagccgctg gacgtgatta cctgggagct ggtgagcgac gtgatgcgcg acatgaagct
     4561 gcagcgccag ggcgacctga cgttccagct gcggcgcttt cccaactttc cgatgctggc
     4621 cggcgtcggc ccgctggacg tgcagctggc cgccatctgt gcccgcatgc cgcagtcgat
     4681 ctcggagctg ctgcgcgcgt ttccgaagca cgagcaggac gtgctgcgct tcgtggtgct
     4741 gtgcgtggtg tcgggcctgg ccaaggtgat tccgggcggg ccggtggcgg ccggcgccgt
     4801 ggcgccgccg cgcgcggcgc aggcccaggc gccggtggcc gcggcggcgc gcaagcccga
     4861 ggtggcggcg cggcgcggct tcttcaaatc gctgatggac aagctgttct aattttttga
     4921 cgccatggcc agtatctaca agctcgtgtt cgccggcccc gtgggggcgg gcaagaccac
     4981 ggccatccaa tccctgtcgg acatcgaggt ggtgcgcacc gaggccaacg cctcggacga
     5041 cgtgaagacc ctcaagaaga ccaccacggt ggcgatggac tacggcctca tgaagctcgc
     5101 cagcggcgac caggtgcggc tgtacggcac accggggcag aagcgctttg acttcatgtg
     5161 ggacatcctg accgagaacg cgctcggcct ggtgctgatg ctgcgcggca actcgcccga
     5221 cccggtggcc gacctgcgca cctacgtgac cgagttccgc gacttcatcg accgcacctc
     5281 gctggtggtc ggcatcacgc attccgacca gggcggctgg cagacgcggc agatggtgtc
     5341 gcgcgagctg gtggccatgg gcttgccgcc gacggccatg gacaccgacg cgcgcagccg
     5401 cgcccacatg gcgacgctgg tcaaggcgct gatctacggc atcgatccgt tcaacaacgc
     5461 ggaagagtga tgaacgaaac ccctgtcagc gccgacgccc ccgcggacga cccgtacctg
     5521 gtgctcacgc ccgccggtgc gctgcacgcc tacggcgagc gcgtgccgga cgagaccagc
     5581 gccatcctgc agaccctgat gccgcgcggc gcctcgctgc gccgcagcgc ctggctggaa
     5641 ctggcgcccg agcaccgcac cgtgctggcg cgcgcgctgt acgaaggctg ggtgcacgag
     5701 gtgcagcgcg agctgcgcgc acccgacgtg cggctcgaca actacctgcc gcatgccatt
     5761 gccgggctgt cgggcacgcg cacggcggcg ctggcctcgg acgaaggctt ctgcctggcg
     5821 cgcgtgggct attcggagga agaggccgaa accctgtgcg tggtggcagc cgatttcttc
     5881 gacttcgcca agcgccagaa gcagcgcggc tggacgggca ccggccgcgc cgtgtcgttc
     5941 cacgaaggca tcgacatgct gctgcccacc accacgctga tgctgttctg ggtggatggc
     6001 accggctact ggctgatcct gggcggtgag ccgctgctca acaaccgcgc cctggtcgag
     6061 ctgatctggg gcatccgcac cgcgggcacc aagtttgcgg tgcgcgagac cacgtcgatc
     6121 gccagccaga ttctggcgat gtaagcgccg ctgccgtggg cggtgcgcgg cctatctcac
     6181 tctgatgatg ggggagtatc ggtcgccagc gcccgtctgt cgggcgctat cggcggccta
     6241 tacggggagt ataggagtgg gccgcgtgcg gcaagccggc gaggcggcgt ccatctcggg
     6301 ccagcccgcg cccacgatta ccgtgtcagg ccagccggcc cagcagcagg tactccatga
     6361 gtgccttctg cacgtgcatt cgattttctg cctcgtccca caccaccgac tgcgggccgt
     6421 cgatgacgtc ggccgtcact tcctcgcccc ggtgggccgg caggcagtgc atgaacagcg
     6481 catcgggctt ggcggcggcc atcatctcgg cgtcgacaca ccagtcggca aaggcctgct
     6541 tgcgcgcctc gttctccgcc tcgtagccca tcgaggtcca cacgtcggtg gtcaccaggt
     6601 cggcgccttc gcaggcctgc atcgggtcgg caaagttgtg ccagtccttg ttgccgccgt
     6661 tgccgtccag cgcggccagg ttggtgtcga cctcgtagcc gcgcggggtg ctcacgcgca
     6721 gctcgaagcc gagcagggca gcggcctgcg cccaggtgtt ggccatgttg ttgccgtcgc
     6781 cgatccaggc caccacccgg ccctggatcg ggccgcggtg ctcgatgtag gtgaagatgt
     6841 cggccaggat ctggcacggg tggtattcgt tggtcaggcc gttgatgacc ggcacgcgcg
     6901 aatgggcggc ga
//
LOCUS       DAOOOG010000137         6900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_13018_length_6900_cov_19.398247, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000137 DAOOOG010000000
VERSION     DAOOOG010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13018_length_6900_cov_19.398247"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1078
                     /locus_tag="PK242_10060"
     CDS             122..1078
                     /locus_tag="PK242_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOZ94404.1"
                     /translation="MGGAALAPSALAGPGRSPVLVLNSLDGTVSVIDPVRWVETGRIA
                     TGKEPHHLYMTPDEKSVIVANAGSDSLTFIDPRTATVQRTLRGIIDPYHLRFSPDMRW
                     FVTAANRLNHIDLYRWDGENPVLAKRIATGKTPSHLWIDSGSTTVWASMQDSNELVAI
                     DLATQQLAHRVKTGPMPADVYVTPDERHLLLGLTGGEGVQVFELATGQAPRAVGQVPT
                     GKGAHAFRALGDRRHVLVSNRVANTISQIDSTRLAVVADFPAPAGPDCMDVSADGSLI
                     MVGSRWAGRLTLIDVARRQPVHQVRVGKSPHGVWTLDHAARV"
     gene            1092..1892
                     /locus_tag="PK242_10065"
     CDS             1092..1892
                     /locus_tag="PK242_10065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOZ94405.1"
                     /translation="MQRRNAIVTIAAGALWTSARGQNSSEPGAAPLYLTFDTGHMGIA
                     ALVAEVLRRQQVRASFFAANERTQEGEGSLSRHWGPWWKARADEGHELASHTWDHVYW
                     RADLPGREPRFRVRPSAGAFAGREFTWSAEQYCQNIEQASERLAYYSGKKPLPLFRAP
                     GGKTSPCLLAAARACGYAHVGWSPAGFLGDELPSDKYPNRVLLERALKTIRPGDILLA
                     HLGIWSRQDPWAPAVLEPLIEGLKARGFVFRTLRQHPQYAPWIAQQRA"
     gene            1907..2860
                     /locus_tag="PK242_10070"
     CDS             1907..2860
                     /locus_tag="PK242_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOZ94406.1"
                     /translation="MTPADLFASAQQWLFEALIGPAAFRLGLGNLLEDGFVAAGWLLL
                     GLLQIAVLLLVIGPLQRLWPVEPVRDRAAIRVDVLYTLLHRLGLFKLAMFFSVEAWLT
                     DAIGVLRAHGLPTLHLDQLWPGVSDGPLASFVLYLLVFDLLGYLLHRAQHQWDWWWRL
                     HAVHHSQRQMTMWSDSRNHLLDSVITDAVFVVVAILIGVAPSQFVWLVALSQLLENLQ
                     HANLRCSFGRLGERLLVSPRFHRRHHAIGIGHEAGAQGALKGVNFGVLFPLWDALLGS
                     ADFGGRFEPTGIRDQVEQGRDYGRGFWAQQWLGLRRLVGRA"
     gene            2880..3755
                     /locus_tag="PK242_10075"
     CDS             2880..3755
                     /locus_tag="PK242_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HOZ94407.1"
                     /translation="MTLLLDSFWRAVAYCLHPRVIALSLLPLGLLVALSFGLGYLYWA
                     SALAAVTAWLDASVVFSTLWGWLGQLGAGELRHGIAPVLLVLLVAPVLIVLALLLVAL
                     LMTPAVLRLVATRRFATLERHGASSMLGSALWSLGHTVLALAALVLSLPLWLVPPLIL
                     IVPPLIWGWLTYRVMSYDALDAHANSAERRAILRQHRTPLLAMGIVCGYLGAAPALAW
                     ASLALFAVAFAVLIPLAIWIYTLVFAFSALWFTHYCLSALQALRQSAPAAIEIEAIAA
                     TAPAADASAALSHEP"
     gene            3745..4575
                     /locus_tag="PK242_10080"
     CDS             3745..4575
                     /locus_tag="PK242_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOZ94408.1"
                     /translation="MSPEHSTPGFGLIVVGDEILSGKRADKHLPKFVELLAERGLRLA
                     WAEYVGDEPARITATLRRALASGDVVVCTGGIGATPDDHTRQCAAAALGVPLALHPEA
                     RRLIEQRGQDMARQQGVPWEPERADNQQRLNMGVFPQGARIVPNAYNQIPGFTLAGPA
                     RGAIYFVPGFPVMAWPMMAWALDTDWSHCFTRGAWRERSVVLYGAMEAPLTPVMERVE
                     AAHPGVKVFSLPSVDHPEHGRHIELGVKGAPAQVDAAYADLRAWLLPHALSYGPELHR
                     "
     gene            4791..6206
                     /gene="glnA"
                     /locus_tag="PK242_10085"
     CDS             4791..6206
                     /gene="glnA"
                     /locus_tag="PK242_10085"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOZ94409.1"
                     /translation="MSQSVADVMKLVSEAECKFVDFRFTDTRGKEQHVTVPVAAFDED
                     KFSDGHAFDSSSVAGWKGIEASDMLLMPDAGTAFIDPFFDEPTLVLTCDVLEPGDGKP
                     YERDPRSIARRAEAYLKASGIGDTAYFGPEPEFFVFDSVRWSTEPGHSFYRIEEYEAP
                     WNTGAELEGSNRGHRPRTKGGYFPVPPVDSTHDMRAEMALILDALGVPVEVFHHETAG
                     AGQMEIGTKFSTLVERADWTQRLKYVVWNVADRYGKTATFMPKPYHGDNGSGMHVHQS
                     IWKGGKNLFAGKGYAGLSETALFYIGGIIRHARALNAITNPTTNSYKRLVPHFEAPVK
                     LAYSARNRSASIRIPYAANDKARRIEARFPDPMANPYLCFAALMMAGLDGIENKIHPG
                     EAATKDLYHLPPEEDRLVPTVCHSLDQALEALDRDRAFLTRGGVFTDAMLDAYIELKM
                     TEVNRIRVQVNPAEYDMYFSL"
     gene            6369..6899
                     /locus_tag="PK242_10090"
     CDS             6369..6899
                     /locus_tag="PK242_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94410.1"
                     /translation="MKSLLPTLRVSLVAATLAAAAVPAAAQDRIYRCGNEYTNQPKGR
                     PDCKLVEGGNVTIVRGTAPAPAAPAAPAAGGGAAPAARSAGTAPASAPRIDNAEQRKR
                     DADAKAILQQELRRAEARQAELDKEYKGGEPDKIGGEARNHQKYLDRVAELKAAIERN
                     QSDIEGIKRELGRLGP"
BASE COUNT         1037 a         2500 c         2328 g         1035 t
ORIGIN      
        1 cttcaccggg cgcggactta cactcgcgcg ccgccattca ccgctttttt taaggttcat
       61 cgtggggttt gccgaacttc cgactctgac acgccgccaa ctgctggtcg ctggcgccgg
      121 cctgggcggc gcggcgctgg cgccgtccgc gctggccggg cccgggcgct cgccggtgct
      181 ggtgctgaac tcgctcgacg gcaccgtcag cgtgatcgac cccgtccgct gggtggaaac
      241 cggccgcatc gccaccggca aggagccgca ccacctgtac atgacgccgg acgagaagtc
      301 ggtcatcgtc gccaatgccg gcagcgactc gctcaccttc atcgacccgc gcacggccac
      361 ggtgcagcgc acgctgcgcg gcatcatcga cccctatcac ctgcgctttt cgcccgacat
      421 gcgctggttc gtcacggccg ccaaccgcct gaaccacatc gacctgtacc gctgggacgg
      481 cgagaacccg gtgctggcca agcgcatcgc caccggcaag acgcccagcc acctgtggat
      541 cgacagcggc agcaccaccg tgtgggccag catgcaggac agcaacgagc tggtggccat
      601 cgacctggcg acgcagcagc tggcgcaccg cgtcaagacc gggccgatgc cggccgacgt
      661 ctacgtcacg cccgacgagc gccacctgct gctgggcctg accggcggcg agggcgtgca
      721 ggtgttcgag ctggccaccg gccaggcgcc cagggcggtc ggccaggtgc ccaccggcaa
      781 gggcgcgcac gcgtttcgcg cgctgggcga ccgccgccac gtgctggtca gcaaccgggt
      841 cgccaacacc atcagccaga tcgacagcac ccgcctggcc gtggtggccg actttccggc
      901 gccggccggc cccgactgca tggacgtgtc ggccgacggc agcctgatca tggtcggctc
      961 gcgctgggcc ggccggctga cgctgatcga cgtggccagg cggcagccgg tgcaccaggt
     1021 cagggtcggc aagtcgccgc acggcgtgtg gacgctggac catgccgcgc gggtgtgagc
     1081 acggacgcat catgcaacgc cgaaatgcta ttgttacgat agctgctggc gcgctctgga
     1141 caagcgccag aggccaaaat agctctgagc ccggcgcggc accgctgtac ctcacgttcg
     1201 acaccggcca catgggcatt gccgcgctgg tggccgaggt gctgcggcgc cagcaggtgc
     1261 gcgcgagctt ctttgccgcc aacgagcgca cccaggaggg cgagggcagc ctgagccgcc
     1321 actggggccc gtggtggaag gcgcgcgccg acgaaggaca cgagctggcc tcgcacacct
     1381 gggaccacgt ctactggcgc gccgacctgc ccgggcgcga gccgcgcttt cgggtgcgcc
     1441 cctcggccgg cgcctttgcc gggcgcgaat tcacctggag cgccgagcag tactgccaga
     1501 acatcgagca ggccagcgag cgcctggcct actacagcgg caagaagccc ctgccgctgt
     1561 ttcgcgcccc gggcggcaag acctcgccgt gcctgctggc ggcggcccgc gcctgcggct
     1621 acgcgcacgt gggctggtcg ccggccggct tcctgggtga cgagctgccc agcgacaagt
     1681 accccaaccg cgtcttgctg gagcgcgcgc tgaagacgat ccgccccggc gacatcctgc
     1741 tggcgcacct gggcatctgg tcgcgccagg acccctgggc gccggccgtg ctggagccgc
     1801 tgatcgaggg gctgaaggcg cgtggcttcg tgttccgcac cctgcgccag cacccgcaat
     1861 acgcgccctg gatcgcgcag cagcgcgcct gagcaagacc cgcacgatga cccctgccga
     1921 cctgttcgcc agcgcccagc agtggctgtt cgaggcgctc atcgggccgg cggccttccg
     1981 gctcggcctg ggcaacctgc tggaagacgg cttcgtcgcc gccggctggc tgctgctggg
     2041 gctgctacag atcgcggtgc tgctgctggt gatcggcccg ctgcagcgcc tgtggccggt
     2101 cgagccggtg cgcgaccgcg cggccatccg cgtcgacgtg ctctacaccc tgctgcaccg
     2161 cctgggcctg ttcaagctgg cgatgttctt cagcgtcgag gcctggctga ccgacgccat
     2221 tggcgtgctg cgcgcccacg gcctgccgac gctgcacctg gatcagctgt ggcccggcgt
     2281 cagcgacggg ccgctggcca gcttcgtgct gtacctgctg gtgttcgacc tgctcggcta
     2341 tctgctgcac cgcgcgcagc accagtggga ctggtggtgg cggctgcacg ccgtgcacca
     2401 ctcgcagcgc cagatgacca tgtggagcga cagccgcaac cacctgctgg acagcgtcat
     2461 caccgacgcc gtgttcgtcg tcgtcgccat cctgatcggt gtggcgccct cgcagttcgt
     2521 gtggctggtg gccctgagcc agctgctgga aaacctgcag cacgccaacc tgcgctgctc
     2581 cttcggccgc ctgggcgagc gcctgctggt cagcccgcgc tttcaccgcc gccaccacgc
     2641 catcggcatc ggccatgaag ccggcgctca gggcgcgctc aaaggcgtca acttcggggt
     2701 gctgttcccg ctctgggacg cgctgctggg ttcggccgac ttcggcggtc gcttcgaacc
     2761 caccggcatc cgcgaccagg tggagcaggg gcgcgactac ggccgcggct tctgggccca
     2821 gcagtggctg ggcctgcggc ggctggtggg ccgcgcctga acgcttatcc ttgacggaca
     2881 tgacgctgct gcttgactcc ttctggcgcg ccgtcgccta ctgcctgcat ccgcgcgtga
     2941 tcgcgctgtc gctgctgccg ctgggcctgc tggtggcgct cagcttcggg ctgggttacc
     3001 tgtactgggc ctcggcgctg gcggcagtca ccgcctggct cgacgcctcg gtggtgtttt
     3061 ccaccctgtg gggctggctc ggccagctgg gcgctggcga gttgcgccac ggcattgcgc
     3121 cggtgctgct ggtgctgctg gtcgcgccgg tgctgatcgt gctggcgctg ctgctggtgg
     3181 ccctgctgat gacgcccgcc gtgctgcgac tggtggcgac acggcgtttt gcgacgctgg
     3241 agcggcacgg tgccagctcg atgctgggct cggccctgtg gtcgctgggg cacaccgtgc
     3301 tggcactggc ggcgctggtg ctgtcgctgc cgctgtggct ggtgccgccg ctgatcctga
     3361 tcgtgccgcc cctgatctgg ggctggctga cctaccgggt gatgagctac gacgccctcg
     3421 acgcccacgc caacagcgcc gagcggcgcg ccatcctgcg ccagcaccgc acgccgctgc
     3481 tggcgatggg catcgtctgc ggctacctgg gcgcggcgcc agccctggcc tgggcgtcgc
     3541 tggcgctgtt cgccgtggcc ttcgcggtgc tgatcccgct ggcgatctgg atctacaccc
     3601 tggtgttcgc cttctccgcc ctgtggttca cccactactg cctgtcggcg ctgcaagcgc
     3661 tgcgccagag cgcgccagca gctatcgaaa tagaagcgat cgcggccacg gcaccggccg
     3721 ccgacgcatc cgccgccctg tcccatgagc cctgaacact ccacccccgg cttcggcctg
     3781 atcgtcgtcg gcgacgaaat cctgtccggc aagcgcgccg acaagcatct gcccaagttc
     3841 gtcgagctgc tggccgaacg cggcctgcgc ctggcctggg ccgaatacgt gggcgacgag
     3901 ccggcgcgca tcaccgccac gctgcggcgt gccttggcct cgggcgacgt ggtggtgtgc
     3961 accgggggca tcggcgccac cccggacgac catacccgcc agtgcgccgc cgccgccctg
     4021 ggcgtgccgc tggcgctgca ccccgaagcc aggcgcctga tcgagcagcg cgggcaggac
     4081 atggcgcgcc agcagggtgt gccctgggag cccgaacgcg ccgacaacca gcagcggctg
     4141 aacatgggcg tgtttccgca aggcgcccgc atcgtgccca atgcctacaa ccagattccg
     4201 gggtttacgc tggccgggcc tgcgcgaggc gctatctatt tcgtaccggg ttttccggtc
     4261 atggcctggc ccatgatggc ctgggcgctg gacaccgact ggtcgcactg cttcacgcgc
     4321 ggcgcctggc gcgagcgttc ggtggtgctt tatggcgcca tggaggcacc gctcaccccg
     4381 gtgatggagc gcgtggaggc ggcgcacccc ggcgtcaagg tgttcagcct gcccagcgtc
     4441 gaccacccgg aacacgggcg gcatatcgag ctgggcgtca agggcgcgcc ggcgcaggtc
     4501 gacgccgcct atgccgacct gcgcgcctgg ctgctgccgc atgcgctgag ctacgggccc
     4561 gagctgcatc ggtagcggcc agcaggcgtt ggccgtggcg gcggcgtcga aaatccgcgg
     4621 aaaagtgcca ccgaccggcg ccgacggcaa aaaagcggcc tgacaacagg caaaattcgg
     4681 ggcatcgtgc agaccattgg ccgggccccg cccggacggc ggccacgccc agcgtgccgc
     4741 ggtcgtacaa ccgccatccc ccgttcatat ccccctcagg agtacccccg atgagccagt
     4801 cggtcgccga cgtcatgaaa ctggtgagcg aagccgaatg caagttcgtc gacttccgct
     4861 tcaccgacac ccgcggcaag gagcagcacg tcaccgtgcc cgtggccgcc ttcgacgagg
     4921 acaagttcag cgacggccac gccttcgaca gctcctcggt ggccggctgg aagggcatcg
     4981 aggcatccga catgctgctg atgcccgacg ccggcacggc cttcatcgac ccgttcttcg
     5041 acgaacccac cctggtgctg acctgcgacg tgctggagcc cggtgacggc aagccctacg
     5101 agcgcgaccc gcgctccatc gcgcgccgcg ccgaggccta cctcaaggcc agcggcatcg
     5161 gtgacacggc gtatttcggc cccgaacccg aattcttcgt cttcgacagc gtgcgctgga
     5221 gcaccgagcc gggtcacagc ttctaccgca tcgaggaata cgaggcgccc tggaacaccg
     5281 gcgccgaact ggaaggcagc aaccgcggcc accgcccgcg caccaagggc ggctacttcc
     5341 cggtgccgcc ggtggacagc acgcacgaca tgcgcgccga gatggcgctg atcctggacg
     5401 cgctgggcgt gccggtcgag gtgttccacc acgagacggc cggcgccggc cagatggaga
     5461 tcggcaccaa gttcagcacc ctggtcgagc gcgccgactg gacgcagcgc ctgaagtacg
     5521 tggtgtggaa cgtggccgac cgctacggca agaccgccac cttcatgccc aagccctacc
     5581 acggcgacaa cggcagcggc atgcacgtgc accagtcgat ctggaagggc ggcaagaacc
     5641 tgtttgccgg caagggctac gccggcctgt ccgagacggc gctgttctac atcggcggca
     5701 tcatccgcca cgcccgggcg ctcaacgcca tcaccaaccc caccaccaac agctacaagc
     5761 gcctggtgcc gcacttcgag gcgccggtca agctggctta ttcggcgcgc aaccgctcgg
     5821 cctcgatccg cattccgtac gccgccaacg acaaggcgcg ccgcatcgag gcgcgctttc
     5881 ccgaccccat ggccaacccc tacctgtgct tcgcggcgct gatgatggcg ggcctggatg
     5941 gcatcgagaa caagatccat cccggcgagg ccgccaccaa ggacctctac cacctgcccc
     6001 ccgaggaaga caggctggtg cccaccgtgt gccacagcct cgaccaggcg ctggaggcgc
     6061 tggatcgcga ccgcgccttc ctgacgcgcg gcggcgtgtt caccgacgcc atgctcgacg
     6121 cctacatcga gctgaagatg accgaggtca accgcatccg cgtgcaggtc aacccggccg
     6181 agtacgacat gtacttctcg ctgtgacgct gtcgccacaa tgacacggaa atcgcccgaa
     6241 atggacggcc tggggctgcc gagggcgcat tttggggaac aggcgtgccc cggaacggtc
     6301 cacctgccgg atacttgttc gttgaaggcc tatcgcggca gttcgccgcg gccgctaccc
     6361 cgaccgacat gaagtctttg ctgccgaccc tccgtgtatc gcttgtcgcc gcgacgctgg
     6421 ccgccgcggc tgtgcccgcg gcggcccagg accgcatcta ccgctgcggc aacgaataca
     6481 ccaaccagcc caagggccgg ccggactgca agctggtgga gggcggcaac gtcaccatcg
     6541 tgcgcggcac cgcacccgcg cccgcggcgc ccgcggcgcc ggcggctggc ggcggtgcag
     6601 cgcccgcggc acgctccgcc gggacagcac cggcctcggc accgcgtatc gacaacgccg
     6661 agcagcgcaa gcgcgacgcc gacgccaagg ccatcttgca gcaggagctg cgtcgtgccg
     6721 aggcccggca ggccgagttg gacaaggaat acaagggtgg cgagcccgac aagatcggcg
     6781 gcgaggcgcg caaccaccag aaatacctgg accgcgtggc cgaactgaaa gccgccatcg
     6841 agcgcaacca gagcgacatc gagggcatca agcgcgagct gggccggctc ggcccctgag
//
LOCUS       DAOOOG010000138         6764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_13397_length_6764_cov_27.104039, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000138 DAOOOG010000000
VERSION     DAOOOG010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13397_length_6764_cov_27.104039"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PK242_10095"
     CDS             <1..317
                     /locus_tag="PK242_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOZ94411.1"
                     /translation="PKFFLGTDSAPHPAHLKEHASGCAGCYTALSAIELYAEAFEAAG
                     ALQRLEGFAAFHGADFYGLPRHRDRITLRRERWTLPEQLPFGDAVVKPLRGGETLAWR
                     LA"
     gene            complement(455..1909)
                     /gene="cls"
                     /locus_tag="PK242_10100"
     CDS             complement(455..1909)
                     /gene="cls"
                     /locus_tag="PK242_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOZ94412.1"
                     /translation="MHLTDPMLLGHPWTFWLGWAWTLSSVVLTTWIVLQRRAPVSTLA
                     WIMVLNLMPVLGLVVYHYFGPRRVRRQRLKRWHQKAVLMSQTDLAALGAGQPPAPLWA
                     RQHTRLIQAACGIAPSSAHRVELLASGGATLEALLREVTQARRHIHLEYYIFEPDHTG
                     TRLLQALTERARAGVQVRLLVDAIGSARLLSRHGRALLDELRAAGGELAVFHPRRLDR
                     LRPLVNLRTHRKIVVVDGHAGFVGGINLTDDENEQVRPHDAYRDTHLLIRGGAVRWLQ
                     YVFLKDWHYAEGRPPGHDAHLLPEQPPGSLPVQIVASGPDTDMEGMHRAMIDALHLAR
                     QRIWLATPYFVPTEAALTALTNAALRGVQVKLMVPERSDSRIVTAAARSYYDELQAAG
                     VLVFEYRGRVLHAKTLVVDEAYGMVGSANFDNRSFRLNFEVAAVVFDADFNHRLAAMF
                     QADLSHCHLVPMQRHTPPLQRLFESVARLGSPLL"
     gene            complement(1932..2297)
                     /locus_tag="PK242_10105"
     CDS             complement(1932..2297)
                     /locus_tag="PK242_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HOZ94413.1"
                     /translation="MSQTLTLYGIPHCDRVKAARAWLAAQGVSAVFHDFKTRGVPPAR
                     LPRWIDAVGWERLLNRQGSTWRRLDAACQASVCDAASAAALLMQQPSLIRRPVVEWPD
                     GAITVGFAADDWQRRLGRA"
     gene            2343..3644
                     /gene="folC"
                     /locus_tag="PK242_10110"
     CDS             2343..3644
                     /gene="folC"
                     /locus_tag="PK242_10110"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOZ94414.1"
                     /translation="MKTLDDWLAHCERLHPKTIELGLERVRAVAERMALRFDCPVITV
                     AGTNGKGSTCAMLEAILQQAGYRTGLYTSPHLVRFEERCRVRGDAVDASELIAACARV
                     EGARGEIALTYFEFTTLAILDVLARAGLDAVVLEVGLGGRLDAVNLIDTDCAIITSVD
                     IDHAELLGDTREQIGLEKAGILRTGRPAIVSDPLPPQSVIDRAQAIGADLWLAGRDFN
                     YSGDRQQWAWAGRGRRYAGLAYPALRGANQLVNAAGVLAALEALRPRLAVTAQAVRRG
                     LALVELPGRFQVVPGQPALVLDVAHNPHSVAALALNLDAMGFYPTTHAVFGAMADKDL
                     AAMLTRMDPIIDRWYFTDLPLPRAARGAALQAAWQAVSQRRDAASTVCDSPRQALAAA
                     AAAATPADRIVVFGSFHTVGGVLQQGTPRLQAGQPGQDARG"
     gene            3656..4579
                     /locus_tag="PK242_10115"
     CDS             3656..4579
                     /locus_tag="PK242_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOZ94415.1"
                     /translation="MAFFKFRQRGQPQPQPEPAGRSRGKAAATETAQESIDSVRRRAR
                     HRLVGAAVLVLLAVIGFPLLFDTQPRPVVVDAPITIPDKDKTAPLRIPAPVPASASLD
                     EREEVVSAAPPAAPAARASDKADAARPAGSAATAEAPKPDDAKARTKADDAQRAAREE
                     QARQRRDEERAKAQAQARRDADDRSKREDEARARRDQDTRAKPDDAERARALLEGRGN
                     GKDKDKTAAADGQGGRFIVQVGAFAEDTAVREARQKAERAGVRTYTQVVNTAAGPRTR
                     VRVGPFASREEAERAAATLKKAGLAGSVLAL"
     gene            4759..5247
                     /locus_tag="PK242_10120"
     CDS             4759..5247
                     /locus_tag="PK242_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOZ94416.1"
                     /translation="MTAIDWLALTVVVVSLLLGTWRGLLYEVLALAGWVLAFVAARWA
                     AAPVGLWLPMGESPEPLRHAVGFVLVFVATAFACGLLAALARRASRALGVRPVDRSFG
                     ALFGLLRGALLLLLVAVAVRLAALHQQPWWTESVSARWLELALLQLHPFLPESFSKWL
                     SA"
     gene            5291..6763
                     /gene="purF"
                     /locus_tag="PK242_10125"
     CDS             5291..6763
                     /gene="purF"
                     /locus_tag="PK242_10125"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOZ94417.1"
                     /translation="MCGIVGVVSNAPVNQLIYDALLLLQHRGQDAAGIVTQQERKFFM
                     HKARGMVRDVFRTRNMRALPGTTGLGQVRYPTAGSADSEEEAQPFYVGAPFGLVLVHN
                     GNLTNAPALKAELFSTDHRHINTESDSEVLLGVLAHELGQATRDAELTPDAVFAAVTR
                     VHQRVRGSYAVVALIAGRGLLAFRDPNGIRPLCFGHGADGAVMVASESVALEGTGFAL
                     ARDVAPGEALYVTLDGQVHARACAPAARLSPCIFEYVYLARPDSVLDGISVYQARLRL
                     GETLAKRVVSTVPPDEIDVVIPIPESSRPSANELARLLGKPYREGFVKNRYVGRTFIM
                     PGQAVRKKSVRQKLNPIGSEFAGRNVLLVDDSIVRGTTSREIVQMARDAGARKVFMAS
                     AAPPVRYPNVYGIDMPTREELVAHGRTDDQVRQLIGADALIYQDVQAMKRAVAALNPA
                     VQGFDASCFDGVYVTGDLDDADVDRLSRQRHGQPVPAVAA"
BASE COUNT          945 a         2442 c         2434 g          943 t
ORIGIN      
        1 gcccgaagtt ctttcttggc accgacagcg ccccgcaccc ggcgcacctg aaggagcacg
       61 ccagcggctg cgccggctgc tacaccgcgc tgtcggccat cgagctgtac gccgaggcct
      121 tcgaagccgc cggcgcgctg cagcggctgg agggctttgc cgccttccac ggcgccgact
      181 tctacggcct gccgcgccac cgcgaccgca tcacgttgcg gcgcgagcgc tggacgctgc
      241 ccgagcagct gccgttcggc gacgcggtgg tcaagcccct gcgcggcggc gagacgctgg
      301 cctggcggct ggcctgatcg cggcgccgtc aatcagggcc aaatcatgct ctggcgcgct
      361 cccagagcgc gccagcagct atatgatttg tagtgtgggc ctgctggatt cagccggtgg
      421 ctgtcttgaa tttcaagctt ccgaagccat gggttcatag cagcggcgag cccagccgcg
      481 ccaccgactc gaacaggcgc tgcagcggcg gcgtgtggcg ctgcatgggc accaggtggc
      541 agtgcgacag gtcggcctgg aacatggcgg ccagccggtg gttgaagtcg gcatcgaaca
      601 ccaccgccgc cacctcgaag ttgagccgaa agctgcggtt gtcgaagttg gcgctgccga
      661 ccatgccgta ggcctcgtcc accaccaggg tcttggcgtg cagcacgcgg ccgcggtatt
      721 cgaacaccag cacgccggcg gcctgcagct cgtcgtagta cgagcgcgcg gcggcggtga
      781 cgatgcgcga gtcgctgcgc tcgggcacca tcagcttgac ctgcacgccg cgcagcgcgg
      841 cgttggtcag cgccgtcagc gccgcctcgg tgggcacgaa gtagggcgtg gccagccaga
      901 tgcgctggcg cgccaggtgc agcgcgtcga tcatcgcgcg gtgcatgcct tccatgtcgg
      961 tgtcgggccc ggaggcgacg atctggaccg gcaggctgcc gggcggctgc tcgggcagca
     1021 ggtgcgcgtc gtggccgggc gggcgacctt cggcgtagtg ccagtccttg aggaacacat
     1081 actgcagcca gcgcacggcg ccgccacgga tcagcaggtg ggtgtcgcgg taggcgtcgt
     1141 gcgggcgcac ctgctcgttc tcgtcgtcgg tcaggttgat gccgccgacg aagcccgcgt
     1201 ggccgtcgac caccacgatc ttgcggtggg tgcgcaggtt gaccagcggg cgcaggcggt
     1261 ccagccgtcg cgggtggaac accgccagct cgccgcccgc ggcgcgcagc tcgtccagca
     1321 gcgcgcggcc gtggcgcgac agcagcctgg ccgagccgat ggcgtccacc agcagccgca
     1381 cctgcacgcc ggcgcgggcg cgctcggtca gcgcctgcag caggcgggtg ccggtgtggt
     1441 ccggttcgaa gatgtagtac tccaggtgaa tgtggcggcg cgcctgcgtc acctcgcgca
     1501 gcagcgcctc cagcgtggcg ccgccgctgg ccagcagctc cacgcggtgc gcgctcgacg
     1561 gtgcgatgcc gcaggcggcc tggatcagcc gggtgtgctg gcgcgcccac agcggcgccg
     1621 gcggctgccc ggcgccgagc gccgccagat cggtctgcga catcagcacc gccttctggt
     1681 gccagcgctt gaggcgctgg cgccgcaccc ggcgcgggcc gaagtagtgg tagaccacca
     1741 gccccagcac cggcatcagg ttcagcacca tgatccaggc cagcgtggac accggcgcgc
     1801 ggcgctgcag cacgatccag gtggtcagca ccacgctcga cagcgtccag gcccagccca
     1861 gccagaaggt ccaggggtgc ccgagcagca tggggtcggt caggtgcatg ggcgcggcgc
     1921 caagggcggg gtcacgcgcg gccgagtcgc cgctgccagt cgtcggccgc gaagccgacc
     1981 gtgatggcgc cgtccggcca ttcgaccacc ggacgccgga tcaggctggg ctgctgcatc
     2041 agcagcgcgg cggcgctggc ggcgtcgcac acgctggcct ggcaggcggc gtccagccgg
     2101 cgccaggtgc tgccctggcg gttcagcagg cgctcccagc ccacggcgtc gatccagcgc
     2161 ggcaggcgcg ccggcggcac gccgcgcgtc ttgaagtcgt gaaacaccgc gctcaccccc
     2221 tgcgcggcca gccaggcacg cgccgccttg acgcggtcgc agtgggggat gccgtacagg
     2281 gtcagggtct ggctcatgct gatcgagcat gatgccatgg tcggtgacaa tcacgtcccg
     2341 tgatgaagac cctggacgac tggctggcgc actgcgagcg cctgcacccc aagaccatcg
     2401 agctgggcct cgagcgcgtg cgcgctgtgg ccgagcgcat ggcgctgcgc ttcgactgcc
     2461 cggtgatcac cgtggccggc accaatggca agggctcgac ctgcgccatg ctggaggcca
     2521 tcctgcagca ggccggctat cgcaccggcc tctacacctc gccccatctg gtgcgcttcg
     2581 aggagcgctg ccgggttcgc ggcgacgccg tggatgcttc cgaattgata gctgcctgcg
     2641 cacgtgtgga gggcgccagg ggcgaaattg ccctgaccta ctttgagttc accacgctcg
     2701 ccatcctgga cgtgctggcg cgcgccgggc tggacgcggt ggttctggag gtgggcctgg
     2761 gcggccggct ggacgcggtc aacctgatcg acaccgactg cgccatcatc accagcgtcg
     2821 acatcgacca tgccgaactg ctgggcgaca cgcgcgagca gatcggcctt gaaaaggccg
     2881 gcatcctgcg caccggccgg ccggccatcg tcagcgaccc gctgccaccg caaagcgtga
     2941 tcgaccgcgc ccaggccatc ggcgccgacc tgtggctggc cgggcgcgac ttcaactaca
     3001 gcggcgaccg ccagcaatgg gcctgggccg gacgcgggcg acgctatgcc gggctggcct
     3061 atccggcgct gcgtggcgcc aaccagttgg tcaacgccgc cggcgtgctg gcggcgctgg
     3121 aggcgttgcg gccgcgcctg gcggtcaccg cccaggcggt gcgccgcggg ctggcgctgg
     3181 tcgagctgcc ggggcgcttt caggtcgtgc ccggccagcc ggcgctggtg ctggacgtgg
     3241 cgcacaaccc gcactcggtg gcggcgctgg cgctcaacct ggacgccatg ggcttttacc
     3301 ccaccaccca cgccgtgttc ggcgccatgg ccgacaagga cctggcggcc atgctgacgc
     3361 gcatggaccc gatcatcgac cgctggtact tcaccgacct gccgctgccg cgcgcggccc
     3421 gtggcgccgc cctgcaggcg gcctggcagg ccgtcagcca gcgccgtgat gcggcgtcca
     3481 cggtgtgcga ctcgccgcgc caggcgctgg ccgcggcggc cgccgccgcc acccccgctg
     3541 atagaatcgt cgtctttggt tcgtttcaca ccgtgggtgg cgtgctgcag cagggcacgc
     3601 cccgcctgca agccggtcag cccggccaag acgcccgcgg ctgacccagc aatccatggc
     3661 tttcttcaag tttcgccagc gcggccagcc tcagccccag cccgaacccg ccgggcgctc
     3721 gcgtggcaag gccgccgcca ccgaaaccgc gcaggagagc atcgacagcg tgcgccggcg
     3781 tgcgcgccac cgcctggtcg gcgccgccgt gctggtgctg ctggcggtga tcggctttcc
     3841 gctgctgttc gacacccagc cgcgtccggt cgtggtcgac gcgcccatca ccatcccgga
     3901 caaggacaag accgccccgt tgcgcattcc cgcgccggta cccgccagcg ccagcctgga
     3961 cgagcgcgaa gaggtggtga gcgccgcccc gccggccgcc cccgccgcgc gtgccagcga
     4021 caaggccgac gccgcgcggc cggccggcag cgcagcgacg gccgaggcac ccaaacccga
     4081 cgacgccaag gccaggacca aggcggacga cgcgcagcgc gccgcacgcg aggaacaggc
     4141 gcgccagcgc cgcgacgagg aacgtgccaa ggcacaagcg caggccaggc gcgacgccga
     4201 cgaccgcagc aagcgcgaag acgaggcccg ggccaggcgc gaccaggaca cccgcgccaa
     4261 acccgacgac gccgagcgcg cccgcgccct gctggaaggc cggggcaacg gcaaggacaa
     4321 ggacaagacc gccgccgccg acggccaggg cgggcgcttc atcgtccagg tgggtgcctt
     4381 cgccgaagac accgccgtgc gcgaggcgcg ccagaaggcc gagcgcgccg gcgtgcgcac
     4441 ctacacccag gtggtcaaca ccgcggccgg cccgcgcacc cgggtgcgcg tcggcccgtt
     4501 tgcctcgcgc gaggaggccg agcgcgccgc cgccacgctg aaaaaggccg ggctggcggg
     4561 cagcgtgctg gcgctctgag agacggtgcg ttcgtccccc catgcccgcc ttgcacgccg
     4621 caacaccccc gcgccccgtg gtcgctgctc gccgcggccg gcaccaaggc ggcgtggtgc
     4681 ggctcgaccg ggtgcgtgcc gacgcacccg tccggcgcgc cggcacgacc cgccgccggt
     4741 gatggcgcag gggtggccgt gacggcgatc gactggctgg cgctgacggt ggtggtggtg
     4801 tcgctgttgc tgggcacctg gcgcggcctg ctgtacgaag tgctggcgct ggccggctgg
     4861 gtgctggcct ttgtggccgc gcgctgggcg gcggcgccgg tcggcctgtg gctgccgatg
     4921 ggcgagtcgc ccgagccgct gcgccacgcc gtcggcttcg tcctggtgtt cgtcgccacc
     4981 gcctttgcct gcggtctgct ggcggcgctg gcccggcgcg ccagccgggc gctgggcgtg
     5041 cgcccggtgg accgcagttt cggcgcgctg ttcggcctgc tgcgtggcgc cttgctgctg
     5101 ctgctggtgg cggtggcggt gcgtctggct gcgctgcacc agcagccctg gtggaccgag
     5161 tcggtcagcg cccgctggct ggagctggcc ctgctgcagc tgcatccatt tctgcccgag
     5221 tcgttctcga aatggctttc ggcctgacaa ttcggcttcg gcccattttg ctttccgcag
     5281 gatcatcacc atgtgcggca tcgtcggcgt tgtcagcaac gcacccgtca accaactgat
     5341 ttacgacgcc ctgctgctgc tgcagcatcg cgggcaggac gccgcgggca tcgtcacgca
     5401 gcaggagcgc aagttcttca tgcacaaggc caggggcatg gtgcgcgacg tgttccgcac
     5461 ccgcaacatg cgcgcgctgc ccggcaccac cgggctgggc caggtgcgtt acccgaccgc
     5521 cggcagtgcc gacagcgagg aagaggcgca gcccttctac gtcggcgccc cgttcggcct
     5581 ggtgctggtg cacaacggca acctgaccaa cgcgccggcg ctcaaggccg aactgttttc
     5641 caccgaccac cgccacatca acaccgagag cgattccgag gtgctgctgg gcgtgctggc
     5701 gcacgagctg gggcaggcca cgcgcgacgc cgagctcacc ccggacgcgg tgttcgccgc
     5761 cgtcacccgc gtgcaccagc gcgtgcgagg ctcctacgcc gtggtggcac tgatcgccgg
     5821 gcgcggcctg ctggcgttcc gcgacccgaa cggcatccgg ccgctgtgct tcgggcacgg
     5881 cgccgacggg gcggtcatgg tggccagcga atcggtggcg ctggaaggca cgggctttgc
     5941 gctggcgcgc gacgtggcgc cgggcgaggc cttgtacgtc acgctcgacg gtcaggtgca
     6001 tgcgcgcgcc tgcgcaccgg cggctcggct gtcgccctgc atcttcgagt acgtgtacct
     6061 ggcgcggccc gactcggtgc tggacggcat ttcggtctac caggcgcgcc tgcgcctggg
     6121 tgaaacgctg gccaagcgcg tggtgtcgac cgtgccgccg gacgagatcg acgtcgtgat
     6181 cccgatcccc gagtccagcc gccccagcgc caacgagctg gcgcgcctgc tgggcaagcc
     6241 gtaccgcgag ggcttcgtca agaaccgcta cgtcggccgc accttcatca tgccgggaca
     6301 ggcggtgcgc aagaaatcgg tgcgccagaa gctcaacccg atcggcagcg aattcgccgg
     6361 tcgcaacgtg ctgctggtgg acgactccat cgtgcgcggc accaccagcc gcgagatcgt
     6421 gcagatggcg cgcgacgccg gggcgcgcaa ggtcttcatg gccagcgcgg cgccgccggt
     6481 gcgctatccc aacgtgtacg gcatcgacat gcccacgcgc gaagagctgg tggcgcatgg
     6541 tcgcaccgac gatcaggtgc gccagctgat cggcgccgac gccctgatct accaggacgt
     6601 gcaagccatg aagcgggcgg tggcggcgct caacccggcc gtgcagggtt tcgacgcctc
     6661 ctgcttcgac ggcgtctacg tcaccggtga cctcgacgac gccgacgtcg atcgcctgag
     6721 ccgccagcgc cacggccagc cagtgccggc ggtggccgcc tgag
//
LOCUS       DAOOOG010000139         6762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_13405_length_6762_cov_21.005368, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000139 DAOOOG010000000
VERSION     DAOOOG010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13405_length_6762_cov_21.005368"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PK242_10130"
     CDS             <1..335
                     /locus_tag="PK242_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOZ94418.1"
                     /translation="ETGETPHAEIVFTIDGREVRGVSDGNGPVDASLKAIESQVSSGA
                     EMVLYSVNAISGNTESQGEVTVRLQNSGRIVNGVGADPDIVVASAKAYLSALNKLHSK
                     ADRVAAQG"
     gene            491..1657
                     /locus_tag="PK242_10135"
     CDS             491..1657
                     /locus_tag="PK242_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOZ94419.1"
                     /translation="MTKHFFVLGIAALLSLSALSTASAAPAKKSVKAAVAHKSASAPA
                     ARSGRPALARQGRETRRTAAQPSGRTTARDTRGARLTLARQESRRTSGLSARQTRLSL
                     RGASTSQVVQARAPRLSFGQMAGLHAVDDPLDLKSSVALVLDQDTQEVLLSKNDHAVL
                     PIASLTKLMTGLVVADARLPLDELVTITEEDIDTEKGSRSRLAVGTQLTRGELMHLAL
                     MSSENRAAHALGRTYPGGLQRFVGLMNARALMLGMKDTRYVEPTGLSSGNRSTAQDLA
                     TLVAAASQQPLLRQLSTSTGYEVAVGNRVVQYNNTNRLVHDPEWQIGLQKTGYISEAG
                     QCLVMQARVAGRKLIMVFLDSSGKHSRLADAERVRRWVEVQRPANTVAGSPNAV"
     gene            complement(1922..2704)
                     /locus_tag="PK242_10140"
     CDS             complement(1922..2704)
                     /locus_tag="PK242_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOZ94420.1"
                     /translation="MRRPRPDAHQTPTIQVIERMFALIDVLASREEPMSLKDISEQTG
                     LHPSTTHRILNDLTIGRFVDRPEAGSYRLGMRLLELGNLVKARLNVRDAALAPMRELH
                     RLIQQPVNLSVRQGDEIVYIERAYSERSGMQVVRAIGGRAPLHLTSTGKLFLASDDPQ
                     RVRAYATRTGLAGHTRNSITQLPALERELIGARHAGVARDNEELELGVRCMAAGIYDD
                     QGKLVAGLSISAPADRLDDAWLPKLKQTAQQISAALGFDERR"
     gene            complement(2710..3456)
                     /locus_tag="PK242_10145"
     CDS             complement(2710..3456)
                     /locus_tag="PK242_10145"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HOZ94421.1"
                     /translation="MTHLKDLPLQALQFYATAPYPCSYLPGRQARSQVATPNHLINND
                     TYSGLVQRGFRRSGLFTYRPYCDGCNACVPVRVRAGEFRPDRSQRRARARHRDLQVRV
                     LKLGYVPEHYRLYLRYQAGRHTGGGMDHDSVDQYTQFLLQSNVNSRLVEFRDPDRDGA
                     PGALRMVAIVDVLGDGLSAVYTFYDPDPRASYGTYAVLWQIEQAIRLGLPHVYLGYWI
                     EGSRKMMYKARFQPLERLEHGQWQRAPRPA"
     gene            complement(3453..4220)
                     /gene="aat"
                     /locus_tag="PK242_10150"
     CDS             complement(3453..4220)
                     /gene="aat"
                     /locus_tag="PK242_10150"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOZ94422.1"
                     /translation="MNAWPPLTQLKPGDPFPPLSQAWPAHSPAPGLVAVGGALDVPSL
                     LAAYAATIFPWFSEGEPILWWSPDPRMVLAVDRFRLHRSLRRVVQRFRASAGCEIRIN
                     SAFDAVIAACAGATRQGQSGTWIGPQIRHAYGALHRAGHAFSVETWIDGELRAGLYGV
                     TIGRAVFGESMFTQVTDGSKIALAALIALCRAQGLPLIDCQQNTRHLAFMGAAEMPRA
                     AFADAVRRLVHEPAPAWRFEPVYWDALLPPAPLTADR"
     gene            complement(4220..4765)
                     /locus_tag="PK242_10155"
     CDS             complement(4220..4765)
                     /locus_tag="PK242_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOZ94423.1"
                     /translation="MLRPPIKHCRQCGTPVQYRIPDDGDTRERAVCPACGTIHYVNPL
                     LVVGTVPYLGERVLLCQRAIEPRWGKWTLPAGFMELDESMAQGAARETAEEAGAQIEM
                     GPFFSAMSIPRIGQVHVFYLARLLSEHFDPGPESAQVHLFTEDEVPWDELAFTAVRRT
                     LECFFADRRRGQFGVHDFDIA"
     gene            4994..5440
                     /locus_tag="PK242_10160"
     CDS             4994..5440
                     /locus_tag="PK242_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOZ94424.1"
                     /translation="MSDRFAFERAPGHLIRRAHQIAVAVFTEEVGAHGVTPVQFAILN
                     ALMQQPGVDQVTLAQRVAFDAATIGSVIGRLEKKGWLRRQADAADRRRRLLWLTAEGE
                     RAALAMIPSVGQVQQRLLQGLDHAERQQLLALLDKLVRHHPRSLSG"
     gene            5494..6207
                     /locus_tag="PK242_10165"
     CDS             5494..6207
                     /locus_tag="PK242_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ94425.1"
                     /translation="MTRRIVVPCRGAVVAVTAGALWLAGCAGMTETQRSTAIGAGVGA
                     VAGGVIGHGRGAVIGAGVGALGGYAWSRYMEGKRQQMQQATAGTGVEVTQTPDNQLKL
                     HIPNDLSFDTNRADLQPRLRPILDQFAQGLGQQPGLEVFIVGHADASGTDAINDPLSL
                     ARANSVRDYLLSRGVDARSMATEGRGSREPLASNDSPAGRARNRRVEIFLAERASVAA
                     APPAAPPPAYSTPVGQPVR"
     gene            6243..>6762
                     /gene="maiA"
                     /locus_tag="PK242_10170"
     CDS             6243..>6762
                     /gene="maiA"
                     /locus_tag="PK242_10170"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HOZ94426.1"
                     /translation="MKLYNYFRSSASFRVRIALHLKGLAYDYLPIHLAKGEQRQPAFA
                     AVTAEGLVPVLQTDDEHRLTQSMAIIEYLDETHPQPPLLPADALGRARVRALAQIVAC
                     EIHPLNNLRVLKYLVHDLKASEDAKNAWYRHWVRLGLQAYEGQLATQTGRYSHGDTPT
                     LADCCLVPQIFNA"
BASE COUNT         1068 a         2290 c         2370 g         1034 t
ORIGIN      
        1 gcgaaaccgg cgagaccccg catgccgaga tcgtgttcac catcgacggc cgcgaggtgc
       61 gcggtgtgtc cgacggcaac ggcccggtgg acgcctcgct gaaggcgatc gaaagccagg
      121 tgtcgagcgg cgccgagatg gtgctctact cggtcaacgc catcagcggc aacaccgaaa
      181 gccagggcga ggtcaccgtg cgtctgcaaa acagcggccg cattgtcaac ggcgtgggtg
      241 ccgaccccga tatcgtggtc gcctcggcca aggcttatct gagtgccctc aataagttac
      301 acagcaaagc tgaccgagtt gctgcgcaag gctagggtta tccccaggaa atccttcgca
      361 gaatgaggaa tgactcgcaa gtcgatgata ttgcgctcct tttttcactt ctgtacactt
      421 gcgccgcaaa ccggacaggc attgaggaag cgtgatggcg gcagcggcga caggcaggcg
      481 ggtggttttt ctgacgaagc atttcttcgt actgggtatc gctgccctgc tgagcctgtc
      541 ggcgctgtcc acggcatcgg cggcaccggc caagaagagc gtcaaagccg ccgtggcgca
      601 caagtccgcc agcgcgcccg ccgcccgcag cggccggcca gccttggcgc gacaggggcg
      661 tgaaacgcga cgcaccgccg cccagccgtc gggtcgcacc acggcgcgcg acacgcgcgg
      721 cgcccgtctg acgctggcca ggcaggaatc ccgtcgcacc agtggcctga gcgcgcgcca
      781 gacgcgcctg tccctgcgcg gcgccagcac atcgcaggtc gtgcaggcgc gggcgccgcg
      841 cctgtccttc ggccagatgg ccggcctgca cgcggtggac gacccgctgg atctgaagtc
      901 cagcgtggcc ctggtgctcg accaggacac gcaggaggtg ctgctgagca aaaacgatca
      961 cgcggtgctg ccgatcgctt cgctgaccaa gctgatgacg ggcctggtgg tggccgatgc
     1021 ccgcctgccg ctggacgagc tggtcaccat caccgaggaa gacatcgaca ccgagaaggg
     1081 cagccgctcg cgcctggccg tgggcaccca gctcacgcgc ggcgagctga tgcacctggc
     1141 gctgatgtcc agcgaaaatc gcgccgctca tgcgctgggc cgcacctatc cgggcggcct
     1201 gcagcgtttc gtgggcctga tgaacgcccg cgccctgatg ctgggcatga aggacacgcg
     1261 ctacgtcgag ccgaccggcc tgtccagcgg caaccgctcc accgcgcagg acctggccac
     1321 cctggtggcc gcggcatccc agcaacccct gttgcgccag ctgtccacct ccaccggcta
     1381 cgaggtggcg gtcggcaacc gcgtggtgca gtacaacaac accaaccgcc tggtgcacga
     1441 cccggagtgg cagatcggcc tgcagaagac cggctacatc tcggaagccg gtcagtgcct
     1501 ggtgatgcag gcgagggtgg cgggccgcaa gctgatcatg gtgtttctcg attccagcgg
     1561 caagcacagc cgtctggccg acgctgaacg cgtgcggcgc tgggtcgagg tgcagcgacc
     1621 ggccaacacc gtggcgggca gcccgaacgc cgtctgatag aaactcgcgt tcaaattgac
     1681 agatgagcag tgacctcgct gcgcttggat gacccatggg ttttgggtca tgcgggggca
     1741 gccggtgtca tggggcatgg ttctgtaggc taggtcgtct gggcgcgtcg ttcgtgcgcg
     1801 ccgccggcat gcgcgtggcc tgacggactt tggcgccgcc gcccgatcgc tacaagtttg
     1861 atagctttcg gcgcaagccg ggcgggcgct ggcgcatgtt tttgttctga tggggtgctt
     1921 ttcagcggcg ttcgtcaaaa cccagcgccg ccgaaatctg ctgcgccgtc tgtttgagct
     1981 tgggcagcca ggcgtcgtcc agccggtcgg ccggggccga gatcgacagc cccgccacca
     2041 gcttgccctg gtcgtcgtag atgccggcgg ccatgcagcg cacgcccagc tccagctctt
     2101 cgttgtcgcg cgccacgccg gcatggcggg cgccgatcag ctcgcgctcg agggccggca
     2161 actgggtgat gctgttgcgg gtgtgaccgg ccaggccggt gcgcgtggcg taggcgcgca
     2221 cgcgctgggg atcgtcagac gccagaaaca gcttgccggt ggacgtcagg tgcagcggcg
     2281 cccgcccgcc gatggcgcgc accacctgca tgcccgagcg ttcgctgtag gcgcgctcga
     2341 tgtagacgat ctcgtcgccc tggcgcacgc tcagattgac cggttgctgg atcagccggt
     2401 gcagctcacg catcggcgcc agcgcggcgt cgcgcacgtt caggcgcgcc ttgaccaggt
     2461 tgcccagctc cagcagccgc atgcccaggc ggtagctgcc ggcctccggc cggtcgacga
     2521 agcgaccgat ggtcaggtcg ttgaggatgc ggtgcgtggt ggaggggtgc agcccggtct
     2581 gctcgctgat gtccttgagc gacatcggct cctcgcgcga agcaagcacg tcgatgagcg
     2641 cgaacatgcg ctcgatcacc tggatggtcg gcgtctggtg ggcgtcgggt ctggggcggc
     2701 gcatgggcgt caggcgggtc ggggggcacg ttgccactgg ccgtgttcca ggcgttccag
     2761 cggctgaaag cgggccttgt acatcatctt gcggctgcct tcgatccagt agcccaggta
     2821 cacatggggc aagcccagcc ggatggcctg ctcgatctgc cacagcacgg cataggtgcc
     2881 gtagctggcg cgcggatcgg ggtcgtagaa agtgtagacg gccgacaggc cgtcgcccag
     2941 cacatcgacg atggccacca tccgcagggc gcccggcgcg ccgtcgcggt ccgggtcgcg
     3001 aaactccacc aggcgggagt tgacgttgct ttgcagcagg aactgggtgt actggtcgac
     3061 gctgtcgtgg tccatgccgc caccggtgtg gcgcccggcc tgatagcgca ggtacaggcg
     3121 gtagtgctcg ggcacatagc cgagcttgag cacgcgcacc tgcaggtcgc ggtggcgtgc
     3181 gcgggcccgg cgctgactgc ggtcgggccg gaattcacct gcccgcacgc gcaccggaac
     3241 acaggcgttg caaccgtcgc agtaggggcg gtaggtgaac agtccgctgc gccgaaaacc
     3301 ccgctgcacc agaccggagt aggtgtcgtt gttgatcagg tggttggggg tggcgacctg
     3361 agagcgtgcc tgccggcccg gcagatagct gcacgggtag ggcgccgtgg cgtagaactg
     3421 cagggcctga agcggaagat ccttgaggtg cgtcaccggt ctgcggtcag cggggctggg
     3481 ggcagcaatg cgtcccagta tacgggctcg aaccgccacg ccggggccgg ttcatgcacc
     3541 aggcgccgca ccgcgtcggc gaaggcggcg cgcggcattt cggccgcgcc catgaaggcc
     3601 agatggcgcg tgttctgctg gcagtcgatc agcggcaggc cctgggcgcg gcacagggcg
     3661 atcagcgccg ccagggcgat cttggagccg tcggtgacct gggtgaacat ggattcgccg
     3721 aacaccgcgc gaccgatcgt caccccgtac aggccggcgc gcagctctcc gtcgatccag
     3781 gtctcgacac tgaaggcgtg gccggcgcgg tgcagtgcgc cgtaggcgtg tcggatctgc
     3841 ggcccgatcc aggtgcccga ttggccctgg cgcgtggcgc cggcgcaggc cgcgatgacg
     3901 gcgtcgaagg cgctgttgat gcggatctcg cagcccgcgc tggcgcgaaa gcgctgcacc
     3961 acccggcgca gcgaacggtg caggcgaaac cgatccaccg ccagcaccat gcgcgggtcg
     4021 gggctccacc acaggatcgg ctcgccttcg ctgaaccagg gaaagatggt ggccgcgtac
     4081 gccgccagca ggctgggcac gtccagcgcg ccgcccacgg cgaccagacc gggcgccggc
     4141 gaatgggccg gccaggcctg cgacagcggc ggaaacgggt cgcccggctt cagctgcgtc
     4201 agcgggggcc aggcgttcat caggcgatat cgaagtcgtg cacgccgaat tgcccgcgcc
     4261 ggcggtcggc gaagaagcac tccagcgtgc ggcgcacggc cgtgaaggcc agctcgtccc
     4321 acggaacctc atcttcggtg aacaggtgca cctgggccga ctcggggccg gggtcgaaat
     4381 gctcgctcag cagccgcgcc agatagaaca cgtgcacctg gccgatgcgt ggaatgctca
     4441 tggcgctgaa gaaggggccc atctcgatct gggccccggc ctcttcggcg gtctcgcgcg
     4501 cggccccctg cgccatgctt tcgtccaact ccatgaagcc ggccggcagg gtccatttgc
     4561 cccagcgcgg ctcgatggcg cgctggcaca gcagcacccg ctcgcccagg tagggcacgg
     4621 tgcccaccac cagcagtggg ttgacgtaat gaatggtgcc gcaggccggg cacacggcac
     4681 gctcgcgcgt gtcgccgtcg tcgggaatgc ggtattgcac cggcgtgccg cactggcggc
     4741 aatgcttgat cggagggcgc agcatggcgc acgcagtgta gttccaagcc agacgcggtg
     4801 gcgtggcggt cgctcgctac actgagaagg tgagaacgaa cccctcatgc ccgctttgac
     4861 cgctccaaca cacccgctcg gcgttgcaca tgctcgccgt ggctcgggct gcggctgcgc
     4921 tttgcgcctt gatcggttgc gttgggacgc tcctctcggg cacccggctg accttcccac
     4981 cttctgaccg ctcatgtcag accgctttgc cttcgagcgg gcgccgggcc atctgatccg
     5041 gcgcgcgcac cagatcgccg tggccgtgtt caccgaagag gtcggcgcgc acggcgtcac
     5101 gccggtgcag ttcgccatcc tcaatgcgct gatgcagcag cccggtgtgg accaggtcac
     5161 actggcgcag cgggtggcgt tcgacgccgc caccatcggc tcggtgatag gccggctgga
     5221 gaaaaagggc tggctgcgac gccaggccga cgcggccgac cggcggcgcc ggctgctgtg
     5281 gctgaccgcc gagggcgagc gcgccgccct cgccatgatc ccctcggtgg ggcaggtgca
     5341 gcagcgcctc ctgcaggggc tggaccatgc cgagcggcag caattgcttg cacttctgga
     5401 caagctggtg aggcatcatc cccgcagcct gtcgggctga gcgggtgctg cgcccgccgt
     5461 ggccacgcca gggtgcagaa aggagtgctg ttcatgaccc gtcgcatcgt tgttccctgt
     5521 cgtggcgccg tggtggccgt caccgccggt gccctgtggc tggccggctg tgccggcatg
     5581 acggaaaccc agcgcagcac cgccatcggc gccggcgtcg gcgcggtggc cggcggtgtg
     5641 atcggccatg ggcgcggcgc cgtcatcggt gccggggtgg gcgcgctggg gggctacgcc
     5701 tggtcccgct acatggaagg caagcgccag cagatgcagc aggccaccgc cggcaccggg
     5761 gtggaggtga cgcagacgcc ggacaaccag ctcaagctgc acatcccgaa cgacctttcg
     5821 ttcgacacca accgtgccga cctgcaaccg cgcctgcggc ccatactcga ccagtttgcg
     5881 cagggcctgg gccagcagcc tggcctggag gtgttcatcg tcggccatgc cgacgccagc
     5941 ggcaccgacg ccatcaacga cccgctgtcg ctggcgcgcg ccaacagcgt gcgcgactac
     6001 ctgctctcgc gcggcgtgga cgcacgctcg atggccaccg aaggccgcgg ctcgcgcgag
     6061 ccgctggcca gcaacgactc gcccgccggc cgggcgcgca accgccgggt cgagatcttc
     6121 ctggccgagc gcgccagcgt ggccgcggcg ccgcccgccg cgccgccacc ggcctacagc
     6181 accccggtcg gccagccggt gcgctgaggc cctgcgggct ggcggcgcta tagtcgttcg
     6241 gcatgaagct ctacaactac ttccgctcgt ccgcgtcctt cagggtgcgc atcgcactgc
     6301 acctgaaggg tctggcctac gactacctgc cgattcacct tgccaaaggt gagcaaaggc
     6361 agcccgcctt cgccgccgtg accgccgaag gcctggtgcc ggtgctgcag accgatgacg
     6421 agcaccggct gacgcagtcg atggcgatca tcgaatacct ggacgaaacc cacccccagc
     6481 caccgctgct gccggccgac gcgctgggcc gcgcccgggt gcgcgcgctg gcacagatcg
     6541 tcgcctgcga gatccatccg ctcaacaacc tgcgcgtgct caagtacctg gtgcacgacc
     6601 tgaaggccag cgaggacgcc aagaacgcct ggtaccgcca ctgggtgcgc ctgggcctgc
     6661 aggcctacga aggccagctg gccacgcaaa ccgggcgcta cagccacggc gacacgccga
     6721 cgctggccga ctgctgcctg gtgccgcaga tcttcaacgc cc
//
LOCUS       DAOOOG010000140         6730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_13505_length_6730_cov_26.093034, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000140 DAOOOG010000000
VERSION     DAOOOG010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13505_length_6730_cov_26.093034"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK242_10175"
     CDS             <1..314
                     /locus_tag="PK242_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOZ94427.1"
                     /translation="FCAIGVYTTNNNTWDIWMMGWFGIVGYIFHKLSMEPAPLLLGLI
                     LGPMMEENLRRALLLSRGDWSILVTRPLSASLLALAAALLVIVLLPAIKKSREEAFVE
                     D"
     gene            446..1777
                     /locus_tag="PK242_10180"
     CDS             446..1777
                     /locus_tag="PK242_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HOZ94428.1"
                     /translation="MPTAHPLRAELHHELHARPPEALSAPLAVLHCVMWADAAARQQS
                     RAHLASLLAAAGLPAPPEGANFHRAQWPELTLRWEQHTEFVAWTFLRPMAAADLQALQ
                     AGAPRDDADGVPAAWLAALPGQCLTRLRLWAVPREAVDGAAAVRALFDASSVTGSTVI
                     SHSSDLHTDMRLQADGSLRMLVLVGEHAPGRVTPRRLGRLVQRLLDIETYRMAALMGL
                     PAARQSVAWLASGEDELARLAQAVQHAAPDDEAALLDRLTRLAAQLEGHYAATHTRFS
                     ASAAYFSLLDQRLAEIAETRIEGMQSLHDFMQRRLMPAMHTCRSAERRQAALSARIER
                     VSGLLRTRVEVEQQQSSRELLAGMNQRQELQLKLQSTVEGLSVAAISYYVLGLLGYLL
                     KGAQRVGWPFSAEASIAAAVPLVVLAVWWSLRRLHHRIVGHGAPTTDRSSP"
     gene            1801..2715
                     /locus_tag="PK242_10185"
     CDS             1801..2715
                     /locus_tag="PK242_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ94429.1"
                     /translation="MAPAGQAPAATNTVAFGLLLAALGAIGFSGKAIIVKLAYRWGVD
                     AITLLMYRMLMALPFFVLMAWWGGRGRPALSRTETRAVLGLGFCGYYLASMLDFIGLQ
                     YITASLERLILYLTPTLVLLCGWALRARPLSAGHLLATAVSYGGALLVFGMEVRDTPG
                     AATAWGAFWVFLSAVSYALYLFFSGEFVARIGSLRLVGLASVVASLCCIAHFLLVNPW
                     QAARVAEPVLWLSLLNSTLCTALPVLLVMMGVERIGASLAAQVGMIGPLSTIAMGFVV
                     LDEPLTPWLAVGTLLVVAGIYLFGRAGR"
     gene            2761..3558
                     /locus_tag="PK242_10190"
     CDS             2761..3558
                     /locus_tag="PK242_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ94430.1"
                     /translation="MDLGIAGKWALVCGASKGLGLGCAQALAGEGVNLLMVARGESAL
                     AQAAAEIRAAGADRAGVSVEFVAQDITTPEGRAAVLARRAEFDIVVTNAGGPPTGDFR
                     DWDRQAWIRALDANMLTPIELIRATLDGMLARGFGRIVNITSSAVKAPIDILGLSNGA
                     RSGLTGFVAGLARNPQIAARGVTINNLLPGKFDTDRLAATFQGAAQKSGQPLEAVRAA
                     QQAQIPARRFGTPAEFGAICAFLCSQQAAYLTGQNILPDGGAYPGSF"
     gene            3622..5049
                     /locus_tag="PK242_10195"
     CDS             3622..5049
                     /locus_tag="PK242_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOZ94431.1"
                     /translation="MTATTCTAAAGDDFIENITFDELAIGQSARLVRTLSQSDIQAFA
                     AVSGDTNPAHLDPAYADATLFQGVIAHGMWGGALISAVLGTQLPGPGTIYLEQALRFS
                     RPVRVGDTLTVTATVLSKDDARKRVELDCQVSNQHGERVLSGVAKVLAPTEKVRQPRT
                     PLPQLRLFDPEARLKAWVAQAAGTPAVHCGVVHPCHPGVLEGVLRAAHAGLIVPQLIG
                     PPERIHAAAEQAGLNLQGVEIIEAPHSDAAAVVAAQRAADGRLEALIAGSLALSELLD
                     AVRAQTALHTRRRLSHVFRLDVPGHARPLWLTDAMLHAAPTLADKADIARNAIDLLRV
                     MGVEQPRVAVLSASEQVDAAVPSTIDAAALCKMVDAGQIGGGLIDGPLAFDSAVSPAS
                     AQGKHPRSAVAGQADVLLAPDMETGSMLARQLLYLAGATACGVIVGARVPITVVTHRL
                     DSANAYLASAVLAKRLALHHRLVRP"
     gene            complement(5050..5568)
                     /locus_tag="PK242_10200"
     CDS             complement(5050..5568)
                     /locus_tag="PK242_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94432.1"
                     /translation="MIPNLSEDELMLLATLPQAIGSCVATAAGSGVLGTGKELFASGQ
                     AVMAGLKDYPDNALIQALVPDPSAADKHGELAQARATRDWALARLKERNIRTPAQMAA
                     LTLEDARAVALLLDSRVEPAQAAQYRQWAMSVAEKVAMAATEGGFLGFGGTRLSEAEQ
                     ALIAQLRAALGA"
     gene            complement(5626..6528)
                     /locus_tag="PK242_10205"
     CDS             complement(5626..6528)
                     /locus_tag="PK242_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ94433.1"
                     /translation="MAHPPLTLKNAALLTLPPLLWAGNAVLGRLVVDLVPPATLNFLR
                     WLLAFFILLPLAGWVLRPGSGLWPHWRRFAVLGLLGVGLYNTLQYLALHTSTPVNVTL
                     VASSMPLWMLALGRLFFNAPVRRPQLLGALLSMLGVTVVLARGDLANLARLQLVPGDV
                     YILAATLAWALYSWLLARPAEPAAIRADWAALLLAQVTFGLGWSGLLAGGEWLLTDAQ
                     IRWGWPLAGALVFVACGPAVLAYRCWGLGVQRAGPALAGFFSNLTPVFAALLSTVLLG
                     EAPRDYHALAFGLIVLSIWVSSRR"
     gene            6616..>6730
                     /locus_tag="PK242_10210"
     CDS             6616..>6730
                     /locus_tag="PK242_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HOZ94434.1"
                     /translation="MPIAVQIERPGGAEEMKLVDLPVGQPGPGQVRIRHHAI"
BASE COUNT          949 a         2478 c         2364 g          939 t
ORIGIN      
        1 tgttctgcgc catcggcgtg tacaccacca acaacaacac ctgggacatc tggatgatgg
       61 gctggttcgg catcgtcggc tacatcttcc acaagctgtc gatggagccg gcgccgctgc
      121 tgctgggcct gatcctgggc ccgatgatgg aagaaaacct gcgccgtgcg ctgctgctgt
      181 cgcgcggcga ctggagcatt ctggtgacgc gcccgctgtc ggcctcgctg ctggcgctgg
      241 cggcggcgct gctggtgatc gtgctgctgc cggcgatcaa gaagagccgc gaggaagcct
      301 tcgtcgaaga ctgacgcccg cccgcctgcg ggccccaagc cagaacgaag aacggcaccc
      361 ccgcgggtgc cgttcttgct tggcggcgac ggtgccggcc ggactccgac acaatgcagc
      421 ccgatccgcc catgcacacc acctcatgcc caccgcgcac cccctgagag ccgaactgca
      481 ccacgaactg cacgcccgcc cgcccgaagc cctgagcgcg ccgctggcgg tgctgcattg
      541 cgtgatgtgg gccgacgccg ctgcgcgcca gcagagccgc gcccatctgg ccagtctgct
      601 ggctgccgcc ggtctgccgg cgccgccgga aggcgccaac ttccatcgcg cccagtggcc
      661 cgagctgacg ctgcgctggg aacagcacac cgagttcgtc gcctggactt ttttgcggcc
      721 gatggcggcg gccgacctgc aagcactgca ggccggcgca ccgcgcgacg atgccgacgg
      781 cgtgcccgcg gcctggctgg ccgcattgcc cggtcagtgc ctgacccggc tgcgcctgtg
      841 ggcggtgccg cgcgaggccg tcgacggcgc tgccgccgtg cgcgcgctgt tcgacgccag
      901 cagcgtcacc ggctccaccg tcatcagcca cagctccgac ctgcacaccg acatgcgcct
      961 gcaggccgac ggcagcctgc gcatgctggt gctggtgggc gagcacgcgc ccggccgcgt
     1021 gacgccgcgc cgcctggggc ggctggtgca gcgcctgctg gacatcgaaa cctaccgcat
     1081 ggcggcgctg atgggcctgc cggcggcgcg ccagtcggtg gcctggctgg ccagcggcga
     1141 agacgagctg gcgcggctgg cgcaggcggt gcagcacgcc gcgccggacg acgaggcggc
     1201 gctgctggat cgcctgacgc gcctggcggc gcagctggaa ggccactacg ccgccacgca
     1261 cacgcgcttt tcggccagcg cagcctactt cagcctgctc gatcagcggc tggccgaaat
     1321 cgccgaaacc cgcatcgaag gcatgcagtc gctgcacgac ttcatgcagc ggcgcctgat
     1381 gccggccatg cacacctgcc gttcggccga acggcgccag gccgcgctgt cggcgcgcat
     1441 cgagcgcgtc agcggcctgc tgcgcacgcg cgtcgaggtc gagcagcaac agtccagccg
     1501 cgagctgctg gccggcatga accagcgcca ggagctgcag ctgaagctgc aatcgacggt
     1561 cgaaggcctg tcggtggcgg cgatctccta ctacgtgctg ggcctgctgg gctacctgct
     1621 caagggggcg cagcgcgtcg gctggccgtt cagcgccgaa gccagcatcg ccgccgcggt
     1681 gccgctggtg gtgctggccg tgtggtggtc gctgcgccga ctgcaccacc gcatcgtcgg
     1741 gcacggcgcg ccgaccacgg accggtcatc gccgtgacgc attctgcatc caaaccggct
     1801 ctggcgcccg ccggacaagc gccagcagct acaaatacgg tagcattcgg gctgctgctg
     1861 gcggccctgg gggcgatcgg cttcagcggc aaggcgatca ttgtcaagct ggcctaccgc
     1921 tggggcgtgg acgccatcac gctgctgatg taccgcatgc tgatggcgct gccgttcttc
     1981 gtgctgatgg cctggtgggg cgggcgcggg cggccggcgc tgtcgcgcac cgaaacgcgg
     2041 gccgtgctgg ggctgggttt ttgcggctac tacctggcca gcatgctgga cttcatcggc
     2101 ctgcagtaca tcaccgccag cctggagcgg ctgatcctgt acctcacgcc cacgctggtg
     2161 ctgctgtgcg gttgggccct gcgcgcgcgc ccgctcagcg ccgggcatct gctggccacc
     2221 gcggtcagct acggtggcgc gctgctggtg ttcggcatgg aggtgcgcga cacgcccggg
     2281 gccgccaccg cctggggggc gttctgggtg ttcctgtcgg cggtttcgta tgcgctgtac
     2341 ctgttcttca gcggcgaatt cgtggcccgc atcggcagcc tgcggctggt gggcctggcc
     2401 tcggtggtgg ccagcctgtg ctgcatcgcg catttcctgc tggtcaaccc gtggcaggcg
     2461 gcgcgcgtgg ccgagccggt gctgtggctg tcgctgctca attcgaccct gtgcacggcg
     2521 ctgccggtgc tgctggtgat gatgggggtg gagcgcatcg gcgccagcct ggcggcgcag
     2581 gtgggcatga tcgggccgct gtccaccatc gccatgggtt ttgtcgttct ggacgagccg
     2641 ctgaccccct ggctggcggt cggcacgctg ctggtggtgg ccggcatcta tctgttcggg
     2701 cgcgccgggc gctgacgcgc cgcagccgcc gccacacgtg attgaccaag gagagcacgc
     2761 atggatctgg gcattgcagg caaatgggcg ctggtgtgcg gcgccagcaa ggggttgggc
     2821 ctgggctgcg cacaggcgct ggccggcgag ggcgtgaacc tgctgatggt ggcgcggggc
     2881 gagtcggcgc tggcccaggc tgctgcagaa ataagagctg ccggcgctga ccgggcgggc
     2941 gttagcgtcg aattcgtggc acaggacatc accacgcccg aaggccgcgc ggcggtgttg
     3001 gcgcggcgtg ccgagttcga catcgtggtc accaacgccg gcgggccgcc caccggcgac
     3061 tttcgcgact gggaccgcca ggcctggatc cgcgcgctgg acgccaacat gctcacgccc
     3121 atcgagctga tccgtgccac gctcgacggc atgctggcgc gcggcttcgg gcgcatcgtc
     3181 aacatcacct ccagcgcggt gaaggcgccg atcgacatcc tgggcctgtc caacggcgcg
     3241 cgctccgggc tgacgggttt cgtcgccggg ctggcgcgca acccgcagat cgccgcccgc
     3301 ggcgtgacca tcaacaacct gctgcccggc aagttcgata ccgaccgcct ggccgccacc
     3361 ttccagggcg cggcgcagaa gtcgggccag ccgctggagg cggtgcgcgc cgcccagcag
     3421 gcccagattc cggcgcgccg cttcggcacg ccggccgagt tcggcgccat ctgcgccttt
     3481 ctgtgcagcc agcaggcggc ctacctgacc gggcagaaca tcctgcccga cggcggcgcc
     3541 tatcccggca gcttctgacg tcatcggccc ggcgccacgc cggcgcggcc gtgccgggcc
     3601 agcctgcaac cgaaccttca catgaccgcc accacctgca ccgctgcggc gggcgacgac
     3661 ttcatcgaga acatcacgtt cgacgagctt gccatcgggc agagcgcgcg cctggtgcgc
     3721 acgctgagcc agtccgacat ccaggccttt gccgccgtct cgggcgacac caacccggcc
     3781 cacctcgatc cggcgtacgc cgacgccacc ttgttccagg gcgtgattgc ccacggcatg
     3841 tggggcgggg cactgatatc ggccgtgctg ggcacccagc tgccgggccc gggcaccatc
     3901 tacctggagc aggccctgcg cttttcgcgc ccggtgcggg tgggcgacac actgaccgtc
     3961 accgccaccg tgctgagcaa ggacgacgcc cgcaagcggg tcgagctgga ctgccaggtc
     4021 agcaaccagc atggcgagcg ggtgctgtcc ggcgtggcca aggtgctggc gccgaccgaa
     4081 aaagtgcgtc agccgcgcac cccactgccg cagctgcgcc tgttcgaccc cgaggcgcgg
     4141 ctcaaggcct gggtggcgca ggccgccggc acacccgcgg tgcactgcgg cgtggtccac
     4201 ccgtgccatc ccggggtgct ggagggcgtg ctgcgcgccg cccatgccgg gctcatcgtg
     4261 ccgcagctga tcggcccgcc cgagcgcatc catgccgccg ccgagcaggc ggggctgaac
     4321 ctgcagggcg tggagatcat cgaggccccg cacagcgacg cggcggccgt cgtggcggcg
     4381 cagcgcgcgg ccgatggtcg gctcgaagcc ctgatcgccg gcagcctggc cctgagcgag
     4441 ctgctcgacg ccgtgcgcgc gcagaccgcg ttgcacacgc gccggcgcct gtcgcacgtg
     4501 tttcggctcg acgtgccggg ccacgcccgg ccgctgtggc tgaccgacgc catgctgcac
     4561 gcggcgccca cgctggcaga caaggccgac atcgcgcgca acgccatcga cctgctgcgc
     4621 gtcatggggg tggagcagcc gcgcgtggcg gtgctgtcgg cgtccgagca ggtcgatgcc
     4681 gccgtgcctt ccaccatcga cgccgcggcg ctgtgcaaga tggtcgacgc gggccagatc
     4741 ggcggcggcc tgatcgacgg gccgctggcc ttcgacagcg ccgtctcgcc ggcgtcggcg
     4801 cagggcaagc acccgcgttc ggccgtggcg ggccaggccg acgtgctgct ggccccggac
     4861 atggaaaccg gctccatgct ggcgcgccag ctgctgtacc tggccggcgc caccgcctgc
     4921 ggcgtgatcg tgggcgcgcg cgtgccaatc accgtggtga cgcaccgtct ggacagcgcc
     4981 aacgcctacc tggcctcggc cgtgctggcc aagcgcctgg ccctgcacca ccggctggtg
     5041 cggccctgat caggcgccca gggcggcgcg cagttgcgcg atcagcgcct gctcggcctc
     5101 gctcaggcgg gtgccgccaa agcccagaaa gccgccttcg gtggccgcca tggccacctt
     5161 ctcggccacc gacatcgccc actggcggta ctgcgccgcc tgcgccggct cgacgcggct
     5221 gtccaacagc agtgccaccg cgcgcgcgtc ctccagcgtc agcgccgcca tctgcgccgg
     5281 cgtgcggatg ttgcgctcct tcaggcgcgc cagcgcccag tcgcgcgtgg cgcgcgcctg
     5341 cgccagctcg ccgtgcttgt cggcggcgga cgggtcgggc accagcgcct ggatcagcgc
     5401 gttgtcgggg tagtccttca gccccgccat caccgcctgg cccgaggcga acagctcctt
     5461 gcccgtgccc agcacgccgc tgcccgccgc cgtggcgacg caggagccga tggcctgcgg
     5521 cagcgtggcc agcagcatca gttcgtcttc gctcaggttg gggatcatgg ggcgcctttc
     5581 agggatcggg tagggcaggc cagtgtagac cggcggcggg ccggctcagc gccgcgacga
     5641 cacccagatc gacagcacga tcaacccgaa ggccagcgcg tggtagtcgc gcggagcttc
     5701 gcccagcagc acggtcgaca gcagcgccgc gaacaccggc gtcaggttcg agaaaaagcc
     5761 cgccagcgcc ggcccggcgc gctgcacccc caggccccag cagcggtagg ccagcaccgc
     5821 cggcccgcag gccacgaaca ccagcgcccc cgccagcggc cagccccagc ggatctgcgc
     5881 gtcggtcagc agccattcgc cgcccgccag aaggcccgac cagcccagcc cgaaggtcac
     5941 ctgcgccagc agcaaggcgg cccagtcggc gcggatcgcc gccggttcgg ccggccgcgc
     6001 cagcagccac gaatacagcg cccaggccag tgtggccgcc aggatgtaca cgtcgcccgg
     6061 caccagctgc agccgcgcca ggttggccag atcgccgcgc gccagcacca ccgtcacgcc
     6121 cagcatcgac agcagcgccc ccagcagttg cgggcgcctc accggggcgt tgaagaacag
     6181 ccgccccagc gccagcatcc acagcggcat gctcgacgcc accagtgtca cgttgaccgg
     6241 cgtcgacgtg tgcagcgcca ggtactgcag cgtgttgtac agccccacgc ccagcaggcc
     6301 cagcacggca aagcgccgcc agtggggcca caggccgctg cccgggcgca gcacccagcc
     6361 ggccagcggc agcaggatga agaaggccag cagccagcgc agaaagttca gcgtcgccgg
     6421 cggcaccaga tccaccacca gccgccccag caccgcgttg cccgcccaca gcagcggcgg
     6481 cagggtcagc agggcggcgt tcttcagcgt caggggtgga tgggccatgt agggcgatgt
     6541 tgccacgccg ggcatccccc gcgccggcgt ggcaacatcg cggctttcac gcacccgccc
     6601 ccgaggagat cgcccatgcc catcgccgtc cagatcgagc gccccggcgg cgccgaggaa
     6661 atgaaactgg tcgatctgcc cgtgggccag cccggccccg gccaggtgcg catccgccac
     6721 cacgccatcg
//
LOCUS       DAOOOG010000141         6709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_13555_length_6709_cov_30.790803, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000141 DAOOOG010000000
VERSION     DAOOOG010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13555_length_6709_cov_30.790803"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /gene="recA"
                     /locus_tag="PK242_10215"
     CDS             complement(<1..712)
                     /gene="recA"
                     /locus_tag="PK242_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOZ94435.1"
                     /translation="MDMAVADKNEKAKALQAALAQIEKQFGKGTIMRLGEGEAIEDIQ
                     VVSTGSLGLDVALGVGGLPRGRVIEIYGPESSGKTTLTLQVIAEMQKLGGTCAFIDAE
                     HALDTQYAQKLGVNLSDMLISQPDTGEQALEIVDSLVRSGAIDLVVVDSVAALVPKAE
                     IEGEMGDSLPGLQARLMSQALRKLTATIKKTNCTVIFINQIRMKIGVMFGSPETTTGG
                     NALKFYASVRLDIRRIGTI"
     gene            854..1327
                     /locus_tag="PK242_10220"
     CDS             854..1327
                     /locus_tag="PK242_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOZ94436.1"
                     /translation="MSPDAPEDRWRQTHLGRLLGHAMRRFDARVLQLMAASERAPLAL
                     SNLAARDKVGAAIVHVTRHLPLTGARLTELAAWAGMSKQAMGDLVDQCVAWGLVTREG
                     DPLDARARRIAFTPAGLDWLAAFRDAVAQAEAEFRAAVGTEVATVVALGLEAYAA"
     gene            1464..2138
                     /locus_tag="PK242_10225"
     CDS             1464..2138
                     /locus_tag="PK242_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ94437.1"
                     /translation="MRILIAEDDQVLADGLMRGLRNSGAAVDHVASGSEADAALMTNA
                     EFDLLILDLGLPKMNGLEVLRRLRARGQTLPVLILTAADGVEERVKGLDYGADDYMAK
                     PFALSELEARVRALTRRGMGAASAIIRHGPLEYDQTGRVASIDGKMIELSARELGLLE
                     VLLQRSGRLVSKEQLVELLCEWGEEVSNNAIEVYIHRLRKKIEKGPIRIATVRGLGYC
                     LEKISA"
     gene            2196..3647
                     /locus_tag="PK242_10230"
     CDS             2196..3647
                     /locus_tag="PK242_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HOZ94438.1"
                     /translation="MKLFQRNQRSLFGEILDWMLTPLLLLWPISLALTWLVAQNLANK
                     PFDRALVYNVQALAQFIQVGPRQRARFSLPQPASELLRADDADNVYFQVMGAHGEFLA
                     GERELPAPPAPGLSEDDAALSDMPQLRDAEFRGLPVRVAYLWVPIAGAPQPALVQVAE
                     TREKRSVLATEIIKGVMLPQFAILPLAVLLVWLALVRGIRPLSELESRIRARRPDDLS
                     PLDDHAVPQEVAPLVASVNDLLTRLKDSIATQKRFLADAAHQLKTPLAGLRMQAELAQ
                     REGASEEELKQSLQQIGLASVRATHTVNQLLALARAEGGSAALAHEPCDLAALTIEVV
                     RQAVPTALEHGTDLGYEGPEPGAPHLVRMGNPVLLKEMLRNLVENALHYARTPGTGAA
                     VVTVRLRANPPGGGLALEVEDNGPGVPAAERELVFQPFYRVLGTNVDGTGLGLPIVRE
                     IAEQHGASVSLSEARPGQQPPGARFVVRFMAPA"
     gene            complement(3751..5160)
                     /gene="mnmE"
                     /locus_tag="PK242_10235"
     CDS             complement(3751..5160)
                     /gene="mnmE"
                     /locus_tag="PK242_10235"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOZ94439.1"
                     /translation="MPAAAPDPIVAIATAPGRGAVGIVRVSGAGLGPLAQALTGRVLT
                     PRHASYGPFTEADGSPIDHGLALFFPAPHSYTGEDVLELQAHGGPVVLQLLLARCLQA
                     AAEPDPVRGAPRLPHLRLARPGEFTERAFLNDKLDLAQAEAVMDLIDASTAAAARSAS
                     RSLAGEFSRQIRQLVDGLIQLRLLIEATLDFPEEEIDVLAAHDVPGQLSKLQQTLTQV
                     RARARQGALLREGIQVVIAGQPNAGKSSLLNALAGAELAIVTPIAGTTRDAVRQTIQI
                     DGVPLHVLDTAGLREAGDEVERIGIERAWGHIEQADAVLWLHDLTRQGAPDYAAAEAQ
                     IARGLADRLPAGVPVLHLWNKHDAAPHAAIAPGDIALSARTGEGLDALRRRLLELVGW
                     QGGAGEGLFIARQRHLQALARAGEHLDAAQAHLDGPAALDLLAEELRLAQQALGEITG
                     AFSADDLLGAIFARFCIGK"
     gene            5206..6489
                     /locus_tag="PK242_10240"
     CDS             5206..6489
                     /locus_tag="PK242_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOZ94440.1"
                     /translation="MDRLLIQNVHVPDLRRGTRRAHHQVLVEGGVIRAVTDEALPAAG
                     ARVLDGGGRTLLPGLIDCHAHVTFSQTNLALLADMPQTTLVLRALPILRGMLGRGFTT
                     VRDAGGADWALKQAIDDGLDGSVLGPRLFVSGPALSPTGGHGDFRPRSDRLRPLGCGC
                     QRLASTRVVDGVDGVRRAVRELLQMGADQIKIMSSGGVASPTDPIDALGYSVEETRAI
                     VDEAAARHTYVLAHAYTPAAIRRAVDCGVRSIEHGNLIDAPTAALMAERGAWAVPTLI
                     TYEALAQGGVRLGLGADSAAKIERVRAAGLRSLAIFKAAGVKMALGTDLLGGSHGLQS
                     EELLIRAQVQSNLEVLQSATLIGAELLGQAGQLGEVSPGARADLLLVDGDPLQDLRCL
                     TGQGEGIALVMQAGRIVIDRRSGPTRPPTEGQSGL"
BASE COUNT          952 a         2377 c         2406 g          974 t
ORIGIN      
        1 tgatggtgcc gatgcggcgg atgtccaggc gcaccgaggc gtagaacttg agcgcattgc
       61 cgccggtggt ggtttcgggg ctgccgaaca tgacgccgat cttcatgcgg atctggttga
      121 tgaagatcac cgtgcagttg gttttcttga tggtggccgt cagcttgcgc agcgcctggc
      181 tcatcaggcg cgcctgcagg ccgggcaggc tgtcgcccat ctcgccctcg atttcggcct
      241 tgggcaccag ggccgccacc gagtcgacca ccaccagatc gatggcgccc gagcgcacca
      301 gcgaatccac gatttccagc gcctgctcgc cggtgtcggg ctggctgatc agcatgtccg
      361 acaggttgac gccgagtttt tgcgcatact gcgtatccag cgcgtgctca gcatcgatga
      421 aggcgcaggt gccgcccagt ttctgcattt cggcgatgac ctgcagcgtc agcgtggtct
      481 tgcccgagga ttccggccca tagatctcga tcacccgccc gcgcggcagg ccgcccacgc
      541 ccagtgcgac gtccaggccc agcgaaccgg tggagaccac ctggatgtcc tcgatcgcct
      601 cgccctcgcc caggcgcatg atggtgccct tgccgaattg cttttcgatc tgcgccagcg
      661 cggcctgcag ggccttggct ttctcgttct tgtcggcgac ggccatgtcc atgattttct
      721 ccttgtgtat caagtgctta acgaatcccg gatgcccggc gtggcggctg gctggatgtt
      781 tgaacaggac tgtacatcaa tccattcaaa gacacaaagc tgatttttgg tcagtttgcc
      841 ttatcatttg cgcatgtcac ccgatgcccc tgaagaccgc tggcgccaga cccacctggg
      901 ccgcctgctg ggccacgcca tgcggcgttt cgacgcccgc gtgctgcagt tgatggccgc
      961 cagcgagcgc gcaccgctgg cgctgtccaa cctggccgcg cgcgacaagg tgggcgcggc
     1021 catcgtgcac gtcacgcgcc atctgccgct gaccggcgcg cgcctgaccg agctggcggc
     1081 ctgggccggc atgagcaagc aggccatggg tgacctggtg gaccagtgcg tggcctgggg
     1141 cctggtcacg cgcgagggcg acccgctgga cgcccgggcg cgccgcatcg ccttcacgcc
     1201 tgccgggctg gactggctgg cagcgttccg tgacgccgtg gcgcaggccg aggccgagtt
     1261 ccgcgccgcc gtgggcaccg aggtggccac cgtggtggcg ctggggctgg aggcctacgc
     1321 cgcctgaagc gtgccgcgcg cccggccctg ccggcgcagg gtcaacccca gcgccgcagg
     1381 cgtcggtctc ggccctagaa tttccctcgg cacaaccgag aatcatgacg gccgcctccg
     1441 cagccgcccc ataggagaca agcatgcgca tcctcatcgc cgaagacgac caggtgctgg
     1501 ccgatggcct gatgcgcggg ttgcgcaatt ccggcgccgc cgtcgaccac gtggccagcg
     1561 gcagcgaagc cgacgccgcg ctgatgacca acgccgagtt cgacctgctg atcctcgatc
     1621 tgggcctgcc caagatgaac ggcctggaag tgctgcgccg cctgcgcgcg cgcgggcaga
     1681 cgctgccggt gctgatcctg accgccgccg acggcgtgga agagcgcgtc aagggcctgg
     1741 actacggcgc cgacgactac atggccaaac cgttcgcgct gtccgagctg gaggcgcgcg
     1801 tgcgcgcgct cacgcgccgc ggcatggggg cggccagcgc catcatccgc cacggcccgc
     1861 tggaatacga ccagaccggc cgcgtagcca gcatcgacgg caagatgatc gagctgtcgg
     1921 cgcgcgagct gggcctgctg gaggtgctgc tgcagcgcag cggccggctg gtcagcaagg
     1981 agcaactggt cgagctgctg tgcgaatggg gcgaggaggt cagcaacaac gcgatcgagg
     2041 tctacatcca ccgcctgcgc aagaagatcg agaaggggcc gatccgcatc gccaccgtgc
     2101 gcggactcgg ctactgtctt gaaaagatat cagcctgaaa gcgccccagc gcccgcccag
     2161 acagcgcaag caggtataaa aactgaagtg tcccggtgaa actgttccag cgcaaccagc
     2221 gctcgctgtt cggcgagatt ctggactgga tgctcacgcc cctgctgctg ctgtggccca
     2281 tcagcctggc gctgacctgg ctggtggcgc agaacctggc caacaagccg ttcgaccgcg
     2341 cgctggtcta caacgtgcag gcgctggcgc agttcatcca ggtcgggccg cgccagcgcg
     2401 cgcgcttctc gctgccgcag ccggccagcg agctgctgcg cgccgacgac gccgacaacg
     2461 tctacttcca ggtgatgggg gcgcacggcg agttcctggc cggcgagcgc gagctgcccg
     2521 cgccgcccgc acccggcctg agcgaagacg acgccgcgct cagcgacatg ccgcagctgc
     2581 gcgacgccga gttccgcggc ctgccggtgc gcgtggccta cctgtgggtg ccgattgccg
     2641 gcgccccgca gccggcgctg gtgcaggtgg ccgaaacgcg cgaaaagcgc agcgtgctgg
     2701 cgaccgaaat catcaagggc gtgatgctgc cgcagttcgc catcctgccg ctggcggtgc
     2761 tgctggtgtg gctggcgctg gtgcgcggca tccgcccgct gtcggagctg gagtcgcgca
     2821 tccgcgcgcg ccggcccgac gacctgtcgc cgctggacga ccacgccgtg ccgcaggagg
     2881 tggcgccgct ggtggcttcg gtcaacgacc tgctgacgcg gctgaaggac tccatcgcca
     2941 cccagaagcg ctttctggcc gacgccgcgc accagctcaa gaccccgctg gccggcctgc
     3001 gcatgcaggc cgagctggcg cagcgcgagg gcgccagcga ggaggagctc aagcaatcgc
     3061 tgcagcagat cggcctcgcc agcgtgcgcg ccacccacac cgtcaaccag ctgctggcgc
     3121 tggcgcgggc cgaaggcggc agcgcggcgc tggcgcacga gccctgcgac ctggccgcgc
     3181 tgaccatcga agtggtgcgc caagcggtgc cgacggcgct cgaacacggc accgacctgg
     3241 gctacgaagg ccccgagccg ggcgcgccgc acctggtgcg catgggcaac ccggtgctgc
     3301 tcaaggaaat gctgcgcaac ctggtggaaa acgcactgca ctacgcgcgc acccccggca
     3361 ccggcgccgc ggtggtgacg gtgcggctgc gcgccaatcc gccgggcggc ggcctggcgc
     3421 tggaggtcga agacaacggc cccggcgtgc cggcggccga gcgcgagctg gtgtttcagc
     3481 ccttctaccg cgtgctgggc accaacgtcg acggcaccgg cctgggcctg ccgatcgtgc
     3541 gcgagatcgc cgagcagcat ggcgccagcg tcagcctgag cgaggcccgc ccgggccagc
     3601 agccacccgg cgcgcgcttc gtggtgcgct tcatggcgcc ggcgtgacgc ggcgctgccg
     3661 gtcacgccgt cgtcacttga ttattgatag ctgctggtgc ttgctggacg ggcggcgagg
     3721 tcgtttttgg catggattca caacgcggca tcacttaccg atgcaaaagc gcgcaaagat
     3781 ggcgcccagc aggtcgtcgg cgctgaaggc gccggtgatc tcgcccagcg cctgctgcgc
     3841 cagccgcagc tcctcggcca gcaggtcgag cgcggcgggg ccgtccaggt gggcttgcgc
     3901 ggcgtccagg tgctcgccgg cgcgcgccag cgcctgcagg tggcgctggc gcgcgatgaa
     3961 caggccctcg ccggcgccgc cctgccagcc gaccagctcc agcaggcggc ggcgcagcgc
     4021 gtccagcccc tcgccggtgc gcgccgacag ggcgatgtca cccggcgcga tggcggcgtg
     4081 cggcgccgcg tcgtgcttgt tccacaggtg cagcaccggc acccctgcgg gcaggcggtc
     4141 ggccaggccg cgcgcgatct gggcttcggc ggcggcgtag tcgggcgcgc cctggcgcgt
     4201 caggtcgtgc agccacagca cggcgtcggc ctgttcgatg tggccccagg cgcgctcgat
     4261 gccgatgcgt tcgacctcgt cgccggcttc gcgcaggccg gcggtgtcga gcacgtgcag
     4321 cggcacgccg tcgatctgga tggtctggcg caccgcgtcg cgcgtggtgc cggcgatcgg
     4381 cgtgacgatg gccagctcgg cgccggccag cgcgttcagc aggctgctct tgccggcgtt
     4441 gggctggccg gcgatcacca cctgaatgcc ttcgcgcagc aacgcgccct ggcgcgcgcg
     4501 ggcccgcacc tgggtcagcg tttgctgcaa ttttgatagc tgcccgggca catcgtgggc
     4561 ggccaggacg tcgatttctt cttccggaaa atcgagcgtg gcctcgatca gcaggcgcag
     4621 ctggatcagc ccgtccacca gctggcggat ctggcgcgag aactcgcccg ccagcgaacg
     4681 gctggccgag cgcgcggcgg cggcggtgct ggcgtcgatc aggtccatca ccgcctcggc
     4741 ctgggccaga tcgagcttgt cgttgaggaa ggcgcgctcg gtgaattcgc ccggccgcgc
     4801 cagccgcagg tgcggcagcc gtggtgcgcc gcgcaccgga tcgggctcgg ccgccgcctg
     4861 caggcagcgc gccagcaaca gctgcagcac caccgggccg ccgtgcgcct gcagctccag
     4921 cacgtcttcg ccggtgtagg aatgcggggc cggaaagaac agcgccagcc cgtggtcgat
     4981 cgggctgccg tcggcctcgg tgaacggccc gtagctggcg tggcgcggcg tcagcacgcg
     5041 cccggtgagc gcctgcgcca gcggccccag gccggccccg gacacgcgca cgatgcccac
     5101 cgcgccgcgc ccgggggcgg tggcgatggc gacgatgggg tccggcgcag cggctggcat
     5161 ggcatgattg tccgccacct gcctgccctt tcacccgccg ccgccatgga ccgcctcctg
     5221 attcagaacg tgcatgtgcc ggacctgcgc cgcggcaccc ggcgggcgca ccaccaggtg
     5281 ctggtggagg gcggcgtgat ccgcgccgtc accgacgagg cgctgccggc cgccggcgcg
     5341 cgcgtgctgg acggcggcgg gcgcaccctg ctgccggggc tgatcgactg ccacgcccac
     5401 gtcaccttct cacagaccaa cctggccctg ctggccgaca tgccgcagac caccctggtg
     5461 ctgcgcgcgc tgccgatcct gcgcggcatg ctggggcgcg gcttcaccac cgtgcgcgac
     5521 gccggcggcg ccgactgggc gctcaagcag gccatcgacg acggcctgga tggcagcgtg
     5581 ctcgggccac gcctgttcgt cagcggcccg gcgctcagcc cgaccggcgg gcacggcgac
     5641 ttccgcccac gctcggaccg gctgcggccg ttgggctgcg gctgccagcg cctggccagc
     5701 acccgcgtgg tggacggcgt ggacggcgtg cggcgggcgg tgcgcgagct gctgcagatg
     5761 ggggccgacc agatcaagat catgtcctcg ggcggcgtgg cctcgcccac cgacccgatc
     5821 gacgcgctgg gttattcggt cgaggaaacc cgcgccatcg tcgacgaggc ggcggcgcgc
     5881 cacacctacg tgctggcgca cgcctacacg ccggcggcga tccggcgcgc ggtggactgc
     5941 ggggtgcgca gcatcgagca cggcaacctg atcgacgccc ccacggcggc gctgatggcc
     6001 gagcgcggcg cctgggccgt gccgaccctg atcacctacg aggcgctggc gcagggcggc
     6061 gtgcggctgg ggctgggggc cgacagcgcg gccaagatcg agcgcgtgcg cgcggccggg
     6121 ctgcgctcgc tggccatctt caaggccgcg ggcgtgaaga tggccctggg caccgacctg
     6181 ctgggcggct cccacgggct gcagagcgag gagctgctga tccgcgccca ggtgcagtcg
     6241 aacctggagg tgctgcaaag cgccaccctg atcggcgccg aactgctggg ccaggccggc
     6301 cagctgggcg aagtcagccc gggcgcgcgg gccgatctgc tgctggtcga cggcgacccg
     6361 ctgcaggatc tgcgctgcct gaccggccag ggcgaaggca tcgcgctggt gatgcaggcc
     6421 ggacgcatcg tgatcgaccg ccgcagcggc ccgacccgcc cgccgaccga aggtcaatca
     6481 ggcctctgac gccctgtcag caagcgcagg aagctattgt tttgatagtc gcctgccgcc
     6541 gcccacggcc accgccgcct tcccgcggcg gcgcgggagg cggcaccgca gctcaggcgc
     6601 cgatcacttg acggtggtcc agatcgccat cgggcggtcg tcggcgcggt gcaccgtggt
     6661 cacgcccttg cgcatggccc aggggcggat ctggtcgtgc agcggcagg
//
LOCUS       DAOOOG010000142         6642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_13741_length_6642_cov_25.047214, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000142 DAOOOG010000000
VERSION     DAOOOG010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13741_length_6642_cov_25.047214"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1022)
                     /locus_tag="PK242_10245"
     CDS             complement(195..1022)
                     /locus_tag="PK242_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOZ94441.1"
                     /translation="MAALAALTRWLRRAPTPIPDALWHDTLARYPFLQRLSADQRQRL
                     QRLATHFIARKRFTGAHGLEVTDAMAVAVAAQACLPLLHLGPAERPEVALTWYDDFVT
                     IVLHPDDAVARRRVMDEAGVVHHYDEVLAGEAMQHGPVMLSWPAVQGAGMGPGSSVVI
                     HEFVHKMDMQAGGADGAPPLPRGFMGARTARQAAAHWHRTWAPAYERFREQVALAERF
                     GAAAPWLDAYAASAPAEFFAVACEAHFVQPERFAQDYPALSAALRAFFKQNNPPAQD"
     gene            complement(1034..1813)
                     /locus_tag="PK242_10250"
     CDS             complement(1034..1813)
                     /locus_tag="PK242_10250"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOZ94442.1"
                     /translation="MTGRPALDELHASTDWRTVDVISDLHLRPEEPATFDAWARYLAA
                     PGADAVFILGDLFEVWVGDDAAQPGTFEARCGRVLAACPVPVFFLRGNRDFLVGSDFL
                     ARCGVRDLPADPTLLLWAGQRVLLSHGDLLCTDDTAYQQFRAQVRSAEWQRAFATQPL
                     AQRQALARQMRTQSQAHQAGQTEYGHTDAALARRWLLDAGATLLIHGHTHRPADHALG
                     ADAQGRALTQVVLSDWHIDAGARRAQLLRLHADGQRQRIDL"
     gene            complement(1827..2339)
                     /locus_tag="PK242_10255"
     CDS             complement(1827..2339)
                     /locus_tag="PK242_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOZ94443.1"
                     /translation="MSNPQVELHIAGHGVITLELDQAKAPKSVANFLAYVRSGHYDNT
                     VFHRVIKNFMIQGGGFEPGMKQKPTQAPITNEADNGLKNARYSVAMARTSDPHSATAQ
                     FFINVVDNEFLNFKAPTAQAWGYAVFGQVVAGQDVVDAIKAVPTTRKGYHDDVPRDDV
                     VIEKAVVVGA"
     gene            complement(2373..3056)
                     /locus_tag="PK242_10260"
     CDS             complement(2373..3056)
                     /locus_tag="PK242_10260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOZ94444.1"
                     /translation="MTLSRRTLARAAATLTAAAALGTALPAFAADPRVKFVTSEGDFV
                     VELYPDKAPKTVANFLQYVKDKHYDGTVFHRVIKGFMVQGGGFDRNYKEKETRASVIH
                     EGRKSLAAGLRNVTGTIAMARTNDPHSAKAQFFVNVVDNDFLDPVLIPSGDPVAKFEY
                     RGQTYTNVPRATLENHPQLFGYTVFGKVVSGMDTIEKIRNTPTGSGGPFPTDVPQKMV
                     VIESARQVN"
     gene            complement(3150..3704)
                     /locus_tag="PK242_10265"
     CDS             complement(3150..3704)
                     /locus_tag="PK242_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ94445.1"
                     /translation="MTLRPFPFLPTARRALRPLLVVLALVLGAAAAQADEYGEVQALQ
                     RAGQTSEALARADRHIAAHPNDPQMRFVKANLLSAGGRAGEAETLLVQLTRDYPELPE
                     PWNNLAVLYAGRGQLDQAQQALEAALRVNPAYATALENLGDVRLRQASEAYQRARQLD
                     PGSGTRLTPRIDALRGLLGSGATR"
     gene            3908..5284
                     /gene="cysS"
                     /locus_tag="PK242_10270"
     CDS             3908..5284
                     /gene="cysS"
                     /locus_tag="PK242_10270"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOZ94446.1"
                     /translation="MTLRIHNTLTRALEDFSPIEPGHVRLYVCGITVYDLCHIGHARS
                     AVAFDVVQRWLKASGLRVTFVRNITDIEDKIIRRALENGETIRQLTDRMIAEMYRDFD
                     ALGIERPTHDPRATDYVPQMLDIIATLEGKGLAYRADNGDVNYAVRRFAGYGKLSGKT
                     LHDLQAGERVAVSEGKHDPLDFVLWKSAKPSEPEGAKWASPFGLGRPGWHIECSAMSC
                     ALLGNTFDIHGGGADLTFPHHENEIAQSEGANGQPLARLWMHNGFVNIDNEKMSKSLG
                     NFFTIREVLERFDAETIRFFIVRAHYRSPLNYSDVHLQDARAALKRLYTALDAVAPAE
                     VAIDWAHPHAARFKAAMDEDFGTPEAVAVLFDLASEVNRSRRPEDAGLLKALGACLGL
                     LQGDPKAFLRAGSALDEAAIAERILARAAAKQAKDFAEADRIRAALLAEGIVLKDSPA
                     GTTWEVAS"
     gene            5330..5980
                     /locus_tag="PK242_10275"
     CDS             5330..5980
                     /locus_tag="PK242_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOZ94447.1"
                     /translation="MAATKKIANQVATPGYWAEACAQLSKRDRVMKRLIPRFGEACLE
                     SRGDAFVTLARSIVGQQISVKAAQSVWNRFEALSRRVAPAQVLRLKVDDMRAAGLSAR
                     KVEYLVDLALHFDGGRIHVEQWQAMDDEAIIAELVAIRGIGRWTAEMFLMFHLLRPDV
                     LPLDDVGLIRGISVNYFSGEPVSRSEAREVAQAWAPWRSVATWYMWRSQDPVPVAY"
     gene            6033..>6642
                     /gene="accA"
                     /locus_tag="PK242_10280"
     CDS             6033..>6642
                     /gene="accA"
                     /locus_tag="PK242_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HOZ94448.1"
                     /translation="MAKRTFLDFEQPIAELENKIEELRYVQSESAVDISEEIDQLDKK
                     SRQLTKDIYADLTPWQITKIARHPERPYTLDYVRECFTDFVELHGDRHYADDQSIVGG
                     LARFNGNACMVLGHQKGRDTKERAARNFGMTRPEGYRKALRLMKTAEKFRLPVFTFVD
                     TPGAFPGIDAEERGQSEAIGRNIYEMAQLEVPIITTIIGEGGS"
BASE COUNT         1008 a         2191 c         2388 g         1055 t
ORIGIN      
        1 cgcgccttgc ccttgaccca tttgccgttg atcagggcgt cggtcttgag caggctgggg
       61 tccttgagca gggccagcgg ggtgtcgggg gtggtcatgg gggtcgtctc ctgcgaaatc
      121 gcacgattgt aggcagcgct tgccggccgt cagggttgct cgaatgctat tgtaatgata
      181 gctgatggcg cacgtcagtc ctgcgccggc gggttgtttt gcttgaagaa cgcacgcagg
      241 gcggcgctca gggccgggta gtcctgggca aagcgctcgg gctgcacgaa gtgcgcttcg
      301 caggcgacgg cgaagaactc ggccggcgca ctggcggcgt aggcgtcgag ccagggcgcg
      361 gcagcgccga agcgctcggc cagcgcgacc tgctcgcgaa accgctcgta cgccggcgcc
      421 caggtgcgat gccagtgggc ggcggcctgg cgtgccgtgc gcgcgcccat gaagccgcgc
      481 ggcagcggcg gcgcgccgtc ggcgccgccg gcctgcatgt ccatcttgtg cacgaactcg
      541 tggatcacca cgctgctgcc ggggcccatg ccggcgccct gtacggcggg ccagctgagc
      601 atcaccgggc cgtgctgcat ggcctcgccg gccagcacct cgtcgtagtg gtgcaccacg
      661 ccggcctcgt ccatcacgcg ccggcgcgcc accgcgtcgt cggggtgcag cacgatggtg
      721 acgaaatcgt cgtaccacgt cagcgccacc tcgggccgct cggcgggtcc caggtgcagc
      781 agcggcaggc aggcctgcgc cgccaccgcc accgccatgg cgtcggtgac ttccagcccg
      841 tgggcaccgg tgaagcgctt gcgggcgatg aagtgcgtgg ccaggcgctg caggcgctgg
      901 cgctgatcgg cgctcagccg ctgcaggaac gggtagcgcg ccagggtgtc gtgccacagc
      961 gcgtccggga tcggcgtggg cgcgcggcgc agccagcggg tcagggcggc cagcgctgcc
     1021 atgggtgctc agatcacagg tcgatgcgct ggcgctgccc gtcggcgtgc aggcgcagca
     1081 gctgggcgcg gcgcgcgccg gcgtcgatgt gccagtcgct cagcaccacc tgggtcaggg
     1141 cgcggccctg cgcgtcggcg cccagcgcgt ggtcggccgg ccggtgggtg tggccgtgga
     1201 tcagcagcgt ggcgccggcg tccagcagcc agcggcgcgc cagcgcggca tcggtgtggc
     1261 cgtattcggt ctggcccgcc tggtgggcct ggctttgcgt ccgcatctgc cgcgccagcg
     1321 cctggcgctg cgccagcggc tgcgtcgcaa aggcgcgctg ccattcggcg ctgcgcacct
     1381 gggcgcgaaa ctgctggtag gcggtgtcgt cggtgcacag caggtcgccg tggctgagca
     1441 gcacgcgctg gccggcccac agcagcagcg tcgggtcggc cggcaggtcg cgcacgccgc
     1501 agcgcgccag aaaatcgctg ccgaccagaa agtcgcggtt gccgcgcaag aagaacacgg
     1561 gcaccgggca ggccgccagc actcgcccgc agcgcgcttc gaaggtgccg ggctgcgccg
     1621 cgtcgtcacc cacccagacc tcgaacagat cgcccagaat gaacaccgcg tcggcgcccg
     1681 gtgcggccag atagcgcgcc caggcgtcga aggtggcggg ctcttcgggc ctgaggtgca
     1741 ggtcggagat gacatcgacc gtgcgccagt cggtgctggc gtgcagctcg tccagcgccg
     1801 ggcggccggt catcgggcgc gggttgtcag gcgccgacca ccacggcctt ttcgatcacc
     1861 acgtcgtcgc gcgggacatc gtcgtggtag cccttgcggg tggtgggcac ggccttgatg
     1921 gcgtcgacca cgtcctggcc cgccaccacc tggccgaaca cggcgtagcc ccaggcctgg
     1981 gcggtgggtg ccttgaagtt caggaattcg ttgtccacca cgttgatgaa gaactgcgcc
     2041 gtggccgagt gcgggtcgct ggtgcgcgcc atggccacgc tgtagcgggc gttcttcagg
     2101 ccgttgtcgg cttcgttggt gatgggcgcc tgggtgggct tttgcttcat gccgggctcg
     2161 aagccgccgc cctggatcat gaagttcttg atgacgcggt gaaacaccgt gttgtcgtag
     2221 tggccgctgc ggacgtaggc caggaagttg gccaccgact tgggcgcctt ggcctggtcc
     2281 agctccagcg tgatgacgcc gtggccggcg atgtgcagtt cgacttgggg gttgctcatg
     2341 agaggggctt tcgtggtgag ggtgaggaat gttcagttga cctgccgggc cgattcgatc
     2401 accaccatct tctggggcac gtcggtgggg aaggggccgc cgctgccggt gggcgtgttg
     2461 cggatctttt cgatcgtgtc catgccgctg acgaccttgc cgaacacggt gtagccgaac
     2521 agttgggggt ggttttcgag ggtggcgcgc ggcacgttgg tgtaggtctg gccgcggtac
     2581 tcgaacttgg ccaccgggtc gccggagggg atcagcaccg ggtcgagaaa atcgttgtcg
     2641 accacgttga cgaaaaattg cgccttggcc gagtgcgggt cgttggtgcg cgccatggcg
     2701 atggtgccgg tgacgttcct gaggccggcg gcgagcgact tgcggccttc gtggatcacc
     2761 gaggcgcggg tctccttttc cttgtagttt ctgtcaaagc cgccgccctg caccatgaag
     2821 cccttgatga cgcggtggaa gacggtgccg tcgtagtgct tgtccttcac gtactgcagg
     2881 aagttggcga ccgtcttggg ggccttgtcg gggtacagct cgaccacgaa atcgccctcg
     2941 ctggtgacga acttcacgcg cggatcggcg gcgaaggcgg gcagggcggt gcccagggcg
     3001 gcggccgcgg tcagcgtggc ggcggcacgg gcgagggttc ggcgggagag cgtcatgggt
     3061 tcggactccg aggttcagga acagcagaaa aaaacagctt ggtgccggca gcggtgcgcg
     3121 agttccgccg catcgcccgc cgccagcgac taacgggtgg cgccgctgcc cagcaggccg
     3181 cgcagggcgt cgatgcgggg tgtcaggcgc gtgccgctgc ccgggtcgag ctggcgtgcg
     3241 cgctggtagg cctcgctggc ctggcgcagg cgcacgtcgc ccaggttttc cagcgcggtg
     3301 gcgtaggccg ggttgacgcg cagcgcggcc tcgagggcct gctgcgcctg gtccagctgg
     3361 ccgcggccgg cgtacagcac ggccaggttg ttccagggtt cgggcagttc ggggtagtcg
     3421 cgtgtcagct gcaccagcag ggtctcggcc tcgccggcgc ggccgccggc gctcagcagg
     3481 ttggccttga cgaagcgcat ttgcggatcg ttggggtggg cggcgatgtg gcggtcggcg
     3541 cgcgccagcg cttcgctggt ctggccggcg cgctgcagcg cctgcacctc gccatattcg
     3601 tcggcctgcg cggccgcggc gcctagcacc agcgcgagca ccaccagcag cgggcgcagc
     3661 gcgcggcggg cggtcggcag aaaggggaag gggcgcagcg tcatgggcaa tcgggccgcc
     3721 gggcggcgca gcctgggcgg tcggggcgcc gcgtgctggc ccgcacgcgg gtcgcgtctt
     3781 atactgaatg aattgtagcc aagcaggcca gccgggccgc cgccacgcgg agggtgcagc
     3841 ggcagcgccc accggcgtct gcggctcatg tgacctgctg ccgcccgctt tcgcccgctt
     3901 tttttccatg accttgcgca tccacaacac gctgacgcgt gccctggagg acttttctcc
     3961 catcgaaccc ggccacgtgc gcctgtacgt gtgcggcatc acggtgtatg acctgtgcca
     4021 catcggtcat gcgcgctcgg ccgtggcctt cgacgtggtg cagcgctggc tcaaggccag
     4081 cggcctgcgc gtgaccttcg tgcgcaacat caccgacatc gaggacaaga tcatccgccg
     4141 cgcgctggaa aacggcgaaa ccatccgcca gctgaccgac cgcatgatcg ccgagatgta
     4201 ccgcgacttc gacgccctgg gcatcgagcg gcccacgcac gacccgcgcg ccaccgacta
     4261 cgtgccgcag atgctggaca tcatcgccac gctggaaggc aagggcctgg cctatcgggc
     4321 cgacaacggc gacgtcaact acgccgtgcg ccggttcgcc ggctacggca agctgtcggg
     4381 caagacgctt catgatctgc aggccggcga gcgcgtggcc gtcagcgagg gcaagcacga
     4441 cccgctggac ttcgtgctgt ggaagtcggc caagcccagc gaacccgagg gcgccaagtg
     4501 ggccagcccg ttcggcctgg gccgccccgg ctggcacatc gagtgctcgg ccatgagctg
     4561 cgcgctgctg ggcaacacct tcgacatcca cggcggtggc gccgacctca cgttcccgca
     4621 ccacgaaaac gagatcgcgc aaagcgaagg tgcgaacggc cagccactgg cccgcctgtg
     4681 gatgcacaac ggcttcgtca acatcgacaa cgagaagatg tccaagtcgc tgggcaactt
     4741 cttcaccatt cgcgaggtgc tggaacggtt cgacgccgag accatccgct tcttcatcgt
     4801 gcgcgcccac taccgcagcc cgctcaacta cagcgacgtg cacctgcagg atgcgcgcgc
     4861 ggccctcaag cgcctgtaca ccgcgctgga tgcggtggcg cccgccgagg tggcgatcga
     4921 ctgggcccac ccccacgccg cgcgcttcaa ggcggcgatg gacgaggact tcggcacccc
     4981 ggaagcggtg gccgtgctgt tcgatctggc cagcgaggtc aaccgcagcc gccggcccga
     5041 ggacgccggc ctgctcaagg ccctgggcgc ctgcctgggt ctgctgcagg gcgatccgaa
     5101 ggccttcctg cgggctggca gcgcgctgga cgaagccgcc attgccgagc gcatcctggc
     5161 ccgcgcggcc gccaagcagg cgaaggactt tgccgaggcc gaccgcatcc gtgccgccct
     5221 gctggccgaa ggcatcgtgc tcaaggactc gccggccggc accacctggg aggttgcgtc
     5281 gtgatcgggt tcagaatgtt tctgacctcc agcgcaggcg ggtagtgcgg tggcagctac
     5341 aaaaaaaata gcaaaccaag tggccacgcc gggctactgg gccgaggcct gcgcgcagct
     5401 cagcaagcgc gaccgcgtca tgaagcggct catcccgcgc tttggcgagg cctgcctgga
     5461 gtcgcgtggc gatgccttcg tcaccctggc gcgctccatc gtcggccagc agatttcggt
     5521 caaggcggcg cagtcggtgt ggaaccgttt cgaggccctg tcgcgccgcg tggcgccggc
     5581 ccaggtgctc aggctgaagg tggacgacat gcgcgccgcc ggcctgtcgg cgcgcaaggt
     5641 cgagtacctg gtggatctgg cgctgcattt cgacggcggc cgcatccatg tcgagcaatg
     5701 gcaggcgatg gacgacgagg ccatcatcgc cgagctggtg gccatccgcg gcatcggccg
     5761 ctggaccgcc gagatgttcc tgatgttcca tctgctgcgc cccgatgtgc tgccgctgga
     5821 cgacgttggt ctgatccgcg gcatcagcgt caactacttc tccggcgagc cggtcagccg
     5881 cagcgaagcg cgcgaggtgg cgcaggcctg ggcgccctgg cgcagcgtgg ccacctggta
     5941 catgtggcgc tcgcaggatc cggtgccggt cgcctactga ggcgatcgca tccggtaaca
     6001 tcggcagctg ttgcgacacc aaggagccga acttggccaa acggacattt cttgatttcg
     6061 agcagcccat cgcggagctt gaaaacaaga tcgaagagct gcgctacgtg cagagcgaga
     6121 gcgcggtcga catctcggaa gaaatcgacc agctcgacaa gaagagccgc cagctcacca
     6181 aggacatcta tgccgacctg acgccctggc agatcaccaa gatcgcgcgt caccccgagc
     6241 ggccgtacac gctggactac gtgcgcgagt gcttcaccga tttcgtcgaa ctgcacggcg
     6301 accgccacta cgccgacgac cagagcatcg tcggcggcct ggcgcgcttc aacggcaacg
     6361 cctgcatggt gctgggtcac cagaaggggc gcgacaccaa ggagcgcgcc gcgcgcaact
     6421 tcggcatgac ccggcccgag ggctaccgca aggcgctgcg gctgatgaag acggccgaaa
     6481 aattcaggct gccggtgttc accttcgtcg acacgcccgg cgcctttccc ggcatcgacg
     6541 ccgaagagcg cggccagtcc gaggccatcg gtcgcaacat ctacgaaatg gcgcagctcg
     6601 aagtgccgat catcaccacc atcatcggtg aaggcggctc cg
//
LOCUS       DAOOOG010000143         6620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_13813_length_6620_cov_26.253618, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000143 DAOOOG010000000
VERSION     DAOOOG010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13813_length_6620_cov_26.253618"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2394
                     /gene="ileS"
                     /locus_tag="PK242_10285"
     CDS             <1..2394
                     /gene="ileS"
                     /locus_tag="PK242_10285"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOZ94449.1"
                     /translation="VLGEWDKPYKTMDFANEAGELRLFKRLIERGFVYRGLKPVYWCF
                     DCGSALAEAEIEYQDRRSTTVDVAFKAHDPAALAAAFGLPHLDKPAYAVIWTTTAWTI
                     PANQALNLGPHIEYALVDTARGLLLLAKARVADCLQRYGLQGEIIATAMGDKLGGLAF
                     EHPLYEVDAAYRRLAPVYLADYATDSDGTGSVHSAPAYGVEDFNSCVAHGLAHADILN
                     PVQGGGSYAPDFALFGGLDIWKAQPAIIEALDQAGRLLASGKLEHSYPHCWRHKSPVI
                     YRAASQWFVRMDEGEGVFTTDKAPETLRTTALRAIEQTAFYPPSGQARLRDMIAGRPD
                     WVISRQRAWGVPIPFFLHKETDALHPRTLEILDQAAAIVEQGGIEAWSRVTAEEILGP
                     EDAQHYTKSSDILEVWFDSGSTFWHVLCGTHPAEHHEHGPEADMYLEGHDQHRGWFHS
                     SLLLASAIRGRAPYRSLLTHGFTVDGQGRKMSKSLGNAVAPQVTSEKLGAEIIRLWVA
                     ASDYSGDIGIDEKILARVVDSYRRIRNTLRFLLANTSDFDPHKHAVPAAQLLELDRWL
                     LARAAEFQAELLAHYEVYEFHPVVAKLQLFCSEDLGGFYLDVLKDRLYTTAPDAHARR
                     SAQTALWQLTQAMLRWMAPFLSFTAEEAWQLVGTSDSIFLEEYWSFDAPDAALLAKWA
                     RIRAIREQVNKEIEVQREQGAVGSSLQANVVLGAPPDDHALLASLGADLKFVCIVSAI
                     ALEAAGALSVSVSASKDTKCERCWHYLPDVGRHAEHPTLCGRCVSNLFGAGETRTVA"
     gene            2397..2912
                     /gene="lspA"
                     /locus_tag="PK242_10290"
     CDS             2397..2912
                     /gene="lspA"
                     /locus_tag="PK242_10290"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOZ94450.1"
                     /translation="MARAASRGGSLAVWLTLAAVVVVLDQFTKQLILGHYRLGDSTTV
                     TSFFNVVRAHNTGAAFSFLAGAGGWQRWFFIVLALAVAGFIVWLLRAHPGQKLFAFSL
                     SMILGGAVGNVIDRLLHGYVVDFLDFHWPFLAPLFHHGHFPAFNLADAAITAGAIGLM
                     LDELLRVRRGR"
     gene            complement(2926..3678)
                     /locus_tag="PK242_10295"
     CDS             complement(2926..3678)
                     /locus_tag="PK242_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOZ94451.1"
                     /translation="MTPITDWGFYAVAVPAVLLLGVSKSGFGAGFGSLAVPLMALSVS
                     VPQAAAILMPVLLVMDLLGIAAFRKDFDRALLRFMLPWGLLGIVVGTLSFRLLQAHWV
                     AGIVGGFTLLFLAQRLLFAPRADAAPPPRWAGALLTATSGFTSFVAHAGGPPVNAYVI
                     PMGLAPLRFTGTMAFFFFVINLAKWLPYAWLGLLDGRNLGTSLVLLPLAPIGVWIGVR
                     IARRIDQRLFYRFVYLGMLLTGLKLLWDAVAA"
     gene            3787..4437
                     /locus_tag="PK242_10300"
     CDS             3787..4437
                     /locus_tag="PK242_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HOZ94452.1"
                     /translation="MNLLAPLPPGWSELLRTALEVAATLAFALSGVIAAARQRLDVVG
                     VCVVAFAAAFGGGTLRDLLLDQRPFFWVRHIEFVWALLALCLAAMALMRQRELALTER
                     AILWPDAIGLGLFTAIGVDLGLAVGLPALAAVMMGVITSCFGGVLRDVLCNLLPSAFS
                     DHRPYALWSFAGGWLHVGLLAAGLPPWLVLTVTVLFTAGMRLVSVWRGWSLPGWRV"
     gene            complement(4608..5810)
                     /gene="pcaF"
                     /locus_tag="PK242_10305"
     CDS             complement(4608..5810)
                     /gene="pcaF"
                     /locus_tag="PK242_10305"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HOZ94453.1"
                     /translation="MTQAFIVDAIRTPFGRYGGALSGVRTDDLGAIPIRALMARHPRV
                     DWQAVGDVLYGCANQAGEDNRNVARMSGLLAGLPIEVPGATINRLCGSGLDAVGTAAR
                     AIRAGEARLMIAGGVESMSRAPFVMPKAESAFSRANAVYDTTIGWRFVNRLMKAQYGV
                     DSMPETAENVAVDYRIEREAQDRMALASQMKAVAAQQAGHLAREITPVSIAQKKGDPI
                     VVEHDEHPRATSLDKLAALKGVVRPDGTVTAGNASGVNDGACALLLADEATASQYGLT
                     PRARVVGMAVAGVAPRVMGIGPAPATEKVLQLTGLKLAQIDVIELNEAFAAQGLAVLR
                     LLGLADDDERVNAWGGAIALGHPLGASGARLATTAINRLHTTGGRYALCTMCIGVGQG
                     IALVLERV"
     gene            complement(5866..6510)
                     /locus_tag="PK242_10310"
     CDS             complement(5866..6510)
                     /locus_tag="PK242_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOZ94454.1"
                     /translation="MSSYQKRSKDELARRVAQDIPDGAYVNLGIGQPTAVANHIPPGR
                     EIILHSENGILGMGPAPAAGQEDYDLINAGKQPVTLRPGGAFFHHADSFAMMRGGHLD
                     VCVLGAFQVSVTGDLANWSTGEAGAIPAVGGAMDLAVGARQTWVMMDLLTRAGESKIV
                     ARCSYPLTAIGCVRRIYTDLATFACSPQGLRLIDRVEGLSLDELRRLVGQPFAA"
BASE COUNT          968 a         2359 c         2313 g          980 t
ORIGIN      
        1 gtgctgggcg agtgggacaa gccctacaag accatggact tcgccaacga ggcgggcgag
       61 ctgcggctgt tcaagcgcct gatcgagcgc ggcttcgtct accgcgggct gaaacccgtc
      121 tactggtgct tcgactgcgg ctcggccctg gccgaggccg agatcgagta ccaggaccgc
      181 cgcagcacca cggtggacgt ggcgttcaag gcgcacgatc cggctgcgct ggcggcggcg
      241 ttcggcctgc cgcacctgga caagccggcc tacgccgtca tctggaccac caccgcctgg
      301 accatcccgg ccaaccaggc gctcaacctg ggaccgcaca tcgagtacgc gctggtcgac
      361 accgcgcgcg gcctgctgct gctggccaag gcgcgagtgg ccgactgcct gcagcgctac
      421 ggcctgcaag gcgagatcat cgccaccgcc atgggcgaca agctcggcgg tcttgcgttc
      481 gagcacccgc tgtacgaggt cgacgcggcc taccggcgcc tcgcgccggt gtacctggcc
      541 gactacgcca ccgacagcga cggcaccggc agcgtgcact cggcgccggc ctacggcgtc
      601 gaggacttca actcctgcgt cgcccacggc ctggcgcacg ccgacattct gaacccggtg
      661 cagggcggcg gcagctacgc gccggacttc gcgctgttcg gcggtctgga catctggaag
      721 gcgcagccgg cgatcatcga ggcgctggac caggccggcc gcctgctggc cagcggcaag
      781 ctggagcaca gttacccgca ctgctggcgc cacaaatcgc cggtgatcta ccgcgccgcc
      841 tcgcagtggt tcgtgcgcat ggacgagggc gagggcgtgt tcaccaccga caaggcgccc
      901 gagacgctgc gcaccacggc gctgcgcgcc atcgagcaga cggcgttcta cccgccctcg
      961 ggccaggcgc ggctgcgcga catgatcgcc ggccgccccg actgggtgat cagccgccag
     1021 cgcgcctggg gcgtgccgat cccgttcttc ctgcacaagg aaaccgacgc gctgcacccg
     1081 cgcaccctgg agatcctcga ccaggcggcc gccatcgtcg agcagggcgg catcgaggcc
     1141 tggagccgcg tcacggccga ggagatcctg ggccccgagg acgcacagca ctacaccaag
     1201 agcagcgaca tcctggaagt ctggttcgac tcgggctcga ccttctggca cgtgctgtgc
     1261 ggcacgcatc cggccgaaca ccatgagcac ggccccgagg ccgacatgta cctggagggc
     1321 cacgaccagc accgcggctg gttccactcc tcgctgctgc tggccagcgc catccgcggc
     1381 cgcgcgccct accgcagcct gctcacgcac ggcttcacgg tcgacggcca gggccgcaag
     1441 atgagcaaga gcctgggcaa cgcggtggcg ccgcaggtga ccagcgagaa gctcggcgcc
     1501 gagatcatcc gcctgtgggt ggcggccagc gactattcgg gcgacatcgg catcgacgag
     1561 aagatcctgg cgcgcgtggt cgacagctac cgccgcatcc gcaacacgct gcgctttctg
     1621 ctggccaaca ccagcgactt cgacccccac aagcacgccg tgccggcggc gcagctgctg
     1681 gagctcgatc gctggctgct ggcgcgcgcg gccgaattcc aggccgagct gctggcgcac
     1741 tacgaggtct acgaattcca ccccgtggtc gccaagctgc agctgttctg ctccgaagac
     1801 ctgggcggct tctacctcga cgtgctcaag gaccgcctct acaccaccgc gcccgacgcg
     1861 cacgcacggc gcagcgcgca gaccgcgctg tggcagctca cccaggccat gctgcgctgg
     1921 atggcgccgt ttctcagctt caccgccgag gaggcgtggc agctggtggg caccagcgac
     1981 tcgatcttcc tcgaagagta ctggagcttc gacgcccccg acgccgcgct gctggccaag
     2041 tgggcgcgca tccgcgccat ccgcgagcag gtcaacaagg aaatcgaggt gcagcgcgaa
     2101 cagggcgcgg tcggctcgtc gctgcaggcc aacgtggtgc tgggcgcgcc gcccgacgac
     2161 cacgcgctgc tggccagcct gggggccgac ctgaagttcg tgtgcatcgt gtctgctatc
     2221 gctctggaag ctgccggcgc cctgtccgtc agcgtcagcg cctcgaaaga caccaaatgt
     2281 gagcgctgct ggcattacct gcccgacgtc ggccggcacg ccgaacaccc caccctgtgc
     2341 gggcgctgcg tcagcaacct gttcggcgcg ggcgagaccc gcacggtggc ctgacggtgg
     2401 cgcgcgcggc atcgcggggc ggctcgctgg ccgtctggct gacgctggcg gccgtggtgg
     2461 tggtgctgga ccagttcacc aagcagctga tcctgggcca ttaccgactg ggcgacagca
     2521 ccaccgtcac ctccttcttc aacgtcgtgc gggcgcacaa cacgggcgcg gcgttctcct
     2581 tcctggccgg cgccggcggc tggcagcgct ggttcttcat cgtgctggcg ctggcggtgg
     2641 cgggcttcat cgtctggctg ctgcgcgcgc acccggggca gaagctgttc gccttttcgc
     2701 tgtcgatgat cctgggcggc gccgtcggca acgtgatcga ccgcctgctg cacggctacg
     2761 tggtggattt cctggacttc cactggccgt ttctggcgcc gctgttccac cacgggcatt
     2821 tcccggcctt caacctggcc gacgccgcca tcaccgccgg cgccatcggc ctgatgctgg
     2881 acgagttgct gcgggtgcgg cgcgggcgct gaggttcagt gacgctcagg cggccaccgc
     2941 atcccacagc agcttcagcc cggtgagcaa catacccagg tagacgaagc ggtagaacag
     3001 gcgctggtcg atgcgccgcg caatgcgcac gccgatccac acgccgatcg gcgccagcgg
     3061 cagcaacacc agcgacgtgc ccaggttgcg cccgtccagc agccccagcc aggcgtaggg
     3121 cagccacttg gccaggttga tgacgaagaa gaagaacgcc atcgtgccgg tgaagcgcag
     3181 cggtgccagc cccatcggaa tcacgtaggc gttgaccggc ggcccgcccg catgggcgac
     3241 gaagctggtg aagcccgagg tcgccgtcag caacgccccc gcccagcgcg gcggcggcgc
     3301 ggcgtcggcg cgtggcgcaa acagcaggcg ctgggccaga aacagcagcg tgaagccgcc
     3361 cacgatgccc gccacccagt gcgcctgcag cagccggaac gacagcgtgc cgaccacgat
     3421 gcccagcagg ccccagggca gcatgaagcg cagcagcgcg cggtcgaagt ccttgcggaa
     3481 ggcggcgatg cccagcaggt ccatcaccag cagcaccggc atcaggatcg ccgccgcctg
     3541 cggcacgctg acgctgagcg ccatcagcgg caccgccagc gagccgaagc cggcgccgaa
     3601 gccgctcttg ctgacgccca gcagcagcac cgccggcacg gcgaccgcgt agaagcccca
     3661 gtcggtgatc ggagtcatgc gtcagatcgg gtgccggcgc tgttccagtc tgcgccacaa
     3721 gctatgattt tgatagttcg gcgagggcgc ggccgggcgc cgccgcggcg ggcacaatcg
     3781 acgcccatga acctgctcgc accgctgccg cccggctggt cggaactgct gcgcaccgcg
     3841 ctggaagtgg cggccacgct ggccttcgcg ctgtcgggcg tgatcgccgc cgcccgccag
     3901 cggctggacg tggtgggtgt gtgcgtggtg gcctttgccg cggccttcgg tggcggcacg
     3961 ctgcgcgacc tgctgctgga ccagcgcccg tttttctggg tgcgccacat cgagttcgtg
     4021 tgggccctgc tggcgctgtg cctggccgcc atggcgttga tgcgccagcg cgaactggcg
     4081 ctgaccgagc gcgccatcct gtggcccgac gccatcggcc tggggctgtt caccgccatc
     4141 ggcgtcgatc tgggcctggc ggtgggcctg ccggcgctgg cggcggtgat gatgggcgtg
     4201 atcaccagtt gcttcggcgg cgtgctgcgc gacgtgctgt gcaacctgct gcccagcgcc
     4261 ttcagcgacc accggcccta cgccctgtgg tcgtttgccg gcggctggct gcatgtgggt
     4321 ctgctggcgg ccggtctgcc accctggctg gtgctgaccg ttaccgtgct gttcaccgcc
     4381 ggcatgcggc tggtgtcggt gtggcgcggc tggtcgctgc cgggctggcg ggtctgaagc
     4441 gccggcgcct tgcgcagggc gcgtgacggc gctgcaccgg gatgagaccg gcggcatcgg
     4501 tccggcgacg gcgcgccagg cgatggggca gccgcggcgt ttggaggcaa aaaggcctcc
     4561 agcgcttgct agaaaagcgc cgacagctat tatttgaata gcaattttca gaccctctcc
     4621 agcaccaggg cgatgccctg cccgacgccg atgcacatgg tgcacagcgc gtagcgcccg
     4681 ccggtggtgt gcaggcggtt gatggcggtg gtggccaggc gcgcgccgct ggcacccagc
     4741 gggtgaccca gggcgatggc gccgccccag gcgttgacac gctcgtcgtc gtcggccagg
     4801 ccgagcaggc gcagcaccgc caggccctgg gcggcaaagg cttcgttgag ttcgatgacg
     4861 tcgatctgcg ccagcttcag gccggtgagc tgcagcacct tctcggtggc cggcgccggg
     4921 ccgatgccca tgacgcgcgg cgccacaccg gccaccgcca tgccgaccac gcgcgcgcgc
     4981 ggcgtcaggc cgtactggct ggccgtggcc tcgtcggcca gcagcagggc gcaggcgccg
     5041 tcgttgacgc cgctggcgtt gccggcggtc accgtgccgt cgggccgcac cacgcccttg
     5101 agcgcggcca gtttgtcgag gctggtggcg cgcgggtgtt cgtcgtgctc gaccacgatc
     5161 ggatcgccct tcttctgcgc gatcgacacc ggcgtgatct cgcgcgccag gtggcccgcc
     5221 tgctgcgccg ccacggcctt catctggctg gccagggcca tgcggtcctg cgcctcgcgc
     5281 tcgatgcggt agtccaccgc cacgttctcg gccgtctcgg gcatcgaatc gacgccgtac
     5341 tgcgccttca tcagccggtt gacgaagcgc cagccgatgg tggtgtcgta caccgcgttg
     5401 gcgcgcgaga aggcgctttc ggccttgggc atgacaaagg gcgcgcggct catgctctcc
     5461 accccgccgg cgatcatcag gcgcgcttcg ccggcgcgga tggcgcgcgc cgccgtgccg
     5521 accgcgtcca gcccggagcc gcacaggcgg ttgatggtgg cgcccggcac ctcgatcggc
     5581 aggcccgcca gcaggcccga catgcgcgcc acgttgcggt tgtcctcgcc ggcctggttg
     5641 gcgcagccgt acagcacgtc gcccacggcc tgccagtcga cgcgcgggtg gcgcgccatc
     5701 agggcccgga tcgggatggc gcccaggtcg tcggtgcgca cgccggacag cgcgccgccg
     5761 tagcgaccga agggggtgcg gatggcgtcg acgatgaagg cttgggtcat ggcagtctcc
     5821 ttggaaaaca gtgacgggaa aatccgcacc gcgcgtggcg cggcatcagg cggcaaacgg
     5881 ctggcccacc aggcgccgca gttcgtcgag cgacagcccc tcgacgcgat cgatcagccg
     5941 caggccctgc ggcgagcagg cgaaggtggc caggtcggtg tagatgcgcc gcacgcagcc
     6001 gatggcggtc agcgggtagc tgcagcgggc cacgatcttg ctttcgccgg cccgcgtcag
     6061 caggtccatc atgacccagg tctggcgcgc gcccaccgcc aggtccatgg cgccgccgac
     6121 ggccggaatg gcaccggcct cgccggtgct ccagttggcc aggtcgcccg tcaccgacac
     6181 ctgaaaggcg cccagcacgc acacgtccag atggccgccg cgcatcatgg caaagctgtc
     6241 ggcgtggtgg aagaaggcgc cgcccggccg cagggtgacc ggctgcttgc cggcgttgat
     6301 gaggtcgtag tcctcctgcc cggcggccgg cgccgggccc atgcccagaa tgccgttctc
     6361 gctgtgcagg atgatttcgc gcccgggcgg aatgtggttg gccaccgccg tcggctggcc
     6421 gatgcccagg ttgacgtagg cgccgtcggg aatgtcctgc gcgacgcgcc gcgccagctc
     6481 gtccttgctt cgcttctgat agctgctcac gcttaccctc cttgcgctgg cgcccgatca
     6541 ggccgcagac cgcaagccgc cggcagcggt cgccacgcgc ggcacgcgca ccacccggtg
     6601 cacgaagatg ccgggcgtga
//
LOCUS       DAOOOG010000144         6544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_14038_length_6544_cov_25.369086, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000144 DAOOOG010000000
VERSION     DAOOOG010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14038_length_6544_cov_25.369086"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PK242_10315"
     CDS             <1..995
                     /locus_tag="PK242_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOZ94455.1"
                     /translation="PLLLMTWKVGPALACGNTVVVKPSEETPATATLLGEVMNAVGVP
                     KGVYNVVHGFGPGSAGEFLTRHPDVNGITFTGETRTGEAIMKAAAEGIRPVSMELGGK
                     NAAVVFADCDLKVTIDTLQRSCFENAGQVCLGTERVYVERPIYEQVVAALKERAEAMT
                     PGEPFDPATRIGPVISKEHQAKVLSYYQKAREQGATVVTGGGVPDMPPHLKDGSWVQP
                     TIWTGLPETASVITDEIFGPCCHISPFDSEEEVLGKVNANRYGLATAIFTQDIGRANR
                     VAQRIHVGLTWINCWFLRDLRTSFGGSKASGIGREGGIHSFEFYTDLRNVMVKF"
     gene            1062..1844
                     /gene="dmpE"
                     /locus_tag="PK242_10320"
     CDS             1062..1844
                     /gene="dmpE"
                     /locus_tag="PK242_10320"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxopent-4-enoate hydratase"
                     /protein_id="HOZ94456.1"
                     /translation="MDKSQIEQLGDRLFDALTQCTSVPPLTSQHPDITVDDAYAIQQR
                     LMQRRLAAGERVVGKKIGVTSQAVMNMLGVFQPDFGWLTDGMVYNEGEAVPMSRLIQP
                     KAEGEIAFVLKKTLQGPGISVADVLAATEGVMACFEIVDSRIQDWKIKIQDTVADNAS
                     CGVFVLGDRLVDVRDVDLTTCGMVLEKNGEIVATGAGAAALGHPANAVAWLANTLGRH
                     GIALEAGEVVLSGSLGIMVPVAAGDNLRVTIGGIGGCSVRFV"
     gene            1962..2876
                     /locus_tag="PK242_10325"
     CDS             1962..2876
                     /locus_tag="PK242_10325"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HOZ94457.1"
                     /translation="MSKKIKCALIGPGNIGTDLLAKLQRSPVLEPVWMVGIDPDSDGL
                     KRAREMGIKTTDQGVDGLVPHIRKDGVQIVFDATSAYVHAENSRKVTEQGAMMVDLTP
                     AAIGPYCVPPVNLREHVGKREMNVNMVTCGGQATIPMVAAVSRVQPVAYAEIVAAVSS
                     KSAGPGTRKNIDEFTRTTAGAIEKVGGARKGKAIIIINPAEPPLIMRDTVHCLVEGQP
                     DQAAITKSIHDMMAEVQKYVPGYKLVNGPVFDGQRVSVFLEVEGLGDYLPRYAGNLDI
                     MTAAAARTAEMFAEEILAGSLTLEPVDV"
     gene            2890..3933
                     /gene="dmpG"
                     /locus_tag="PK242_10330"
     CDS             2890..3933
                     /gene="dmpG"
                     /locus_tag="PK242_10330"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HOZ94458.1"
                     /translation="MSIQGKKITLHDMTLRDGMHPKQHQMTLAQMKSIAQGLDAAGMP
                     LIEVTHGDGLGGASVNYGFPAHSDEEYLSAVIPLMKQAKVSALLLPGIGTVDHLKMAR
                     ELGVHTIRVATHCTEADVSEQHITIARKMDMDTVGFLMMAHMNSAEGLVKQARLMEGY
                     GANCIYVTDSAGYLLPDQVKERIAAVRAALKPETELGFHGHHNLAMGVANSIAAIEAG
                     ANRIDAAAAGLGAGAGNTPMEVLVAVLERMGVHTGVDVFKIQDVAEDLVVPVMDFPIR
                     IDRDALTLGYAGVYGSFLLFAKRAEKKYGVPAREILVEMGRRGMVGGQEDMIEDTALT
                     LSKKKLAAQVASH"
     gene            3958..4746
                     /gene="dmpH"
                     /locus_tag="PK242_10335"
     CDS             3958..4746
                     /gene="dmpH"
                     /locus_tag="PK242_10335"
                     /EC_number="4.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047437 - 4-oxalocrotonate decarboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-3-hexenedioate decarboxylase"
                     /protein_id="HOZ94459.1"
                     /translation="MTLDPKTLDQLAEHLHACQREARDTLKITDQHPDMDWDDAYAIQ
                     DRILAAKLKGGARVAGYKAGLTSHAKMRQMGVTDPVFGFLVDEFTVADGGEVRVSELI
                     HPKVEPEICFVTRAELKGPGCHIGAVLAACDVVLPGIEVIDSRYRDFKFDVKSVIADN
                     TSAARFVVGGRAVDARRVDLTTCGLVLEKNGVPVALGAGAAVLGHPAAAVAMLVNHLG
                     RRGQSLPAGSLVLSGGATEAVSVQPGDHVSLRVQGMGSVSLRFV"
     gene            4806..5777
                     /locus_tag="PK242_10340"
     CDS             4806..5777
                     /locus_tag="PK242_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ94460.1"
                     /translation="MKRRDFIAAASAATLCHASWAQDAFPARPVKLIVPFPPGGPTDI
                     MGRTAAKAMGDRLGQQFVVENKAGAGGNIGTDAVAKAAPDGYTIGLAAISSLAIAPHL
                     YGKLPFNVDKDLAPISLVGTTPCAIMAGPQAPFSDLKGLIAYAKANPGKLSYATSGIG
                     TSNHLAAELLQSVAGIQLTHVPYKGSSQIVPDLIAGTVPMSMESSLATTLQHVRSGKL
                     KAIAVTSARRAKALPEVPTVAESGYAGFEVETWFGLIAPAATPAAIVSRLQGAWAEGS
                     RGADAQSAFDNISANLRVTTPAEFGAFIQAEHRRWGELIRKLGIKAD"
     gene            5777..6379
                     /locus_tag="PK242_10345"
     CDS             5777..6379
                     /locus_tag="PK242_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOZ94461.1"
                     /translation="MGGAAGRHVPARRLGRAACAALLLSLGALRGAAEPARALGGEAA
                     AAGPSWHARVEFVVDGDSLWVRPERGGRRARLRLQGIDAPELCQPGGASARAALQALV
                     RGERLRVTVHARDRFGRRLASVQRQRDGLDVAAGMVAQGWAWNDRQGWRRGRYAREEA
                     QARAARRGVFAAADAQSPADFRRRHGPCGTAQSGAASTSR"
BASE COUNT         1128 a         2233 c         2236 g          947 t
ORIGIN      
        1 tgccactgct gctgatgacc tggaaggtcg gcccggcgct ggcctgcggc aacacggtgg
       61 tggtcaaacc gtccgaagaa acccccgcca ccgccaccct gctgggcgag gtgatgaacg
      121 ccgtgggtgt gcccaagggc gtgtacaacg tggtgcacgg cttcggcccc ggttcggccg
      181 gcgaattcct gacccggcac cccgacgtca acggcatcac cttcaccggc gagacgcgca
      241 ccggcgaagc catcatgaag gccgccgccg agggcatccg cccggtgtcg atggagctgg
      301 gcggcaagaa cgccgccgtg gtgttcgccg attgcgatct gaaggtcacc atcgacacgc
      361 tgcagcgctc ctgcttcgaa aacgccggcc aggtctgcct gggcaccgag cgcgtctatg
      421 tggagcgacc gatctacgag caggtggtgg cggcgctgaa ggagcgcgcc gaggccatga
      481 cccccggcga gccgttcgac ccggcgacac ggatcggccc ggtcatcagc aaggagcatc
      541 aggccaaggt gctgtcctac taccagaagg cgcgcgaaca gggcgccacc gtggtgaccg
      601 gcggcggcgt gcccgacatg ccgccgcacc tgaaggacgg cagctgggtg caaccgacca
      661 tctggaccgg cctgcccgag accgccagcg tcatcaccga cgagatcttc ggcccctgct
      721 gccacatcag ccccttcgac agcgaagagg aggtgctggg caaggtcaac gccaaccgct
      781 acggactggc gacggcgatc ttcacgcagg acatcggccg cgccaaccgg gtggcgcagc
      841 gcatccacgt cggcctcacc tggatcaact gctggtttct gcgcgacctg cggacctcgt
      901 ttggcggctc caaggcctcg ggcatcggcc gcgaaggtgg catacactcg ttcgagtttt
      961 acaccgatct gcgcaacgtg atggtcaagt tctgagcgat ataggctgct cgcgcttgac
     1021 tggcaagcgc gagcagctct cattttgata gtattgaaac catggacaag tcacagatcg
     1081 agcagttggg cgaccgcctt tttgacgccc tcacgcagtg caccagcgtg ccgccgctga
     1141 ccagccagca ccccgacatc acggtggacg atgcctacgc catccagcag cggctgatgc
     1201 agcgccgcct ggccgccggc gagcgggtgg tgggcaagaa gatcggcgtg accagccagg
     1261 cggtgatgaa catgctgggc gtgttccagc ccgacttcgg ctggctgacc gacggcatgg
     1321 tctacaacga gggcgaggcc gtgcccatga gccgcctgat ccagcccaag gccgagggcg
     1381 aaatcgcctt cgtgctcaag aagaccctgc agggccccgg catcagcgtg gccgacgtgc
     1441 tggccgccac cgaaggcgtg atggcctgct tcgaaatcgt cgactcccgc atccaggact
     1501 ggaagatcaa gattcaggac acggtggccg acaacgcctc ctgcggcgtg ttcgtgctgg
     1561 gcgatcgcct ggtcgatgtg cgcgacgtcg atctgaccac ctgcggcatg gtgctggaga
     1621 agaacggcga aatcgtcgcc accggtgccg gcgccgccgc cctgggccac ccggccaacg
     1681 cggtggcctg gctggccaac accctgggcc gccatggcat tgccctggag gcgggcgagg
     1741 tggtgctgtc gggctcgctg ggcatcatgg tgccagtggc cgcgggcgac aacctgcgcg
     1801 tcacgatcgg cggcatcggc ggctgcagcg tgcggtttgt ttgaacgacg aatgaccttg
     1861 cttcgcgtcc atgacggcgc tgtacgccct gaccgaggcc gtcatttcgt ctttgaagcc
     1921 gcgcgagcgg tgaagtcatt tcatcattgg aaagcatgga aatgagcaag aaaatcaagt
     1981 gtgccctgat cggcccaggc aacatcggca ccgatctgct ggccaagctg cagcgcagcc
     2041 cggtgctgga gccggtgtgg atggtgggca tcgaccccga ctccgacggt ctcaagcgcg
     2101 cgcgcgagat gggcatcaaa accaccgacc agggggtcga tggtctggtg ccgcacatca
     2161 ggaaggacgg cgtgcagatc gtgttcgacg ccaccagcgc ctacgtgcac gctgaaaact
     2221 cgcgcaaggt caccgagcag ggcgcgatga tggtggacct gacgcccgcc gccatcggtc
     2281 cctactgcgt gccgccggtc aacctgcgcg agcacgtggg caagcgcgaa atgaacgtca
     2341 acatggtcac ctgcggcggc caggccacca tccccatggt ggccgccgtc agtcgcgtgc
     2401 agccggtggc ctatgccgaa atcgtggccg ccgtgtccag caagtccgcc ggcccgggca
     2461 cgcgcaagaa catcgacgaa ttcacccgca ccacggcggg cgcgatcgaa aaggtgggcg
     2521 gcgcccgcaa gggcaaggcc atcatcatca tcaacccggc cgagccgccg ctgatcatgc
     2581 gcgacaccgt gcactgcctg gtcgagggcc agcccgatca ggccgccatc accaagagca
     2641 tccacgacat gatggccgag gtgcagaagt atgtgcccgg ctacaagctg gtcaacggcc
     2701 cggtgttcga cggccagcgg gtgtcggtgt tcctggaggt cgagggcctg ggcgactacc
     2761 tgcccaggta cgccggcaac ctcgacatca tgaccgccgc cgccgcacgc accgccgaga
     2821 tgtttgccga agagatcctg gccggctcgc tcaccctcga acccgtcgac gtctgaagga
     2881 gaaccggcca tgagcatcca aggcaagaag atcaccctgc atgacatgac gctgcgcgac
     2941 ggcatgcacc ccaagcagca tcagatgacg ctggcgcaga tgaaatccat cgcccagggc
     3001 ctggacgccg ccggcatgcc gctgatcgag gtcacccacg gcgacggcct gggcggcgcc
     3061 tcggtcaact acggctttcc ggcccacagc gacgaggaat acctctccgc cgtcatcccg
     3121 ctgatgaagc aggccaaggt gtcggcgctg ctgctgcccg gcatcggcac cgtcgaccac
     3181 ctgaagatgg cgcgcgagct gggcgtgcac accatccgcg tggccaccca ctgcaccgag
     3241 gccgatgtca gcgagcagca catcaccata gcccgcaaga tggacatgga caccgtgggc
     3301 tttctgatga tggcgcacat gaacagcgcc gagggcctgg tcaaacaggc caggctgatg
     3361 gaaggttacg gcgccaactg catctacgtg accgactcgg ccggctacct gctgcccgac
     3421 caggtcaagg agcgcatcgc cgccgtgcgc gccgcgctca agcccgagac cgagctgggc
     3481 tttcatggcc accacaacct ggccatgggc gtggccaatt cgatcgccgc catcgaggcc
     3541 ggcgccaacc gcatcgacgc cgccgccgcc ggcctgggcg ccggtgccgg caacacgccg
     3601 atggaggtgc tggtggccgt gctcgagcgc atgggcgtgc acaccggcgt ggacgtgttc
     3661 aagatccagg atgtggccga ggacctggtg gtgccggtga tggacttccc gatccgcatc
     3721 gaccgcgacg cgctcaccct gggctacgcc ggggtgtacg gcagcttcct gctgttcgcc
     3781 aagcgcgccg aaaagaaata cggcgtgccg gcgcgcgaga tcctggtcga gatgggccgc
     3841 cgcggcatgg tcggcggcca ggaagacatg atcgaggaca ccgccctgac gctatcaaaa
     3901 aagaagctgg cggcgcaggt tgcaagccac tgaaggccaa ttgaagttca caacgccatg
     3961 acactcgacc cgaagacgct cgaccagctg gccgagcacc tgcacgcctg ccagcgcgag
     4021 gcgcgcgaca cactgaagat caccgaccag caccccgaca tggactggga cgacgcctac
     4081 gccatccagg accgcatcct ggccgccaag ctcaagggcg gcgcgcgcgt ggcgggctac
     4141 aaggcgggcc tgacctcgca cgccaagatg cgccagatgg gcgtgaccga cccggtgttc
     4201 ggctttctgg tcgacgagtt caccgtcgcc gacggcggcg aggtcagggt gagcgagctg
     4261 atccatccca aggtggaacc tgagatctgt ttcgtcaccc gcgctgagct gaaaggccct
     4321 ggctgccaca tcggcgccgt gctggccgcc tgcgatgtgg tgctgcccgg catcgaagtc
     4381 atcgacagcc gctaccgcga cttcaaattc gacgtgaaaa gcgtgattgc agacaacacc
     4441 tcggccgcgc gcttcgtcgt cggtggtcgt gcggtggacg cgcgccgcgt cgatctcacg
     4501 acctgcggcc tggtgctgga gaaaaacggc gtgccggtgg ccctgggcgc cggcgccgcc
     4561 gtgctgggcc acccggccgc cgccgtggcc atgctggtca accacctggg ccggcgcggc
     4621 cagagcctgc cggccggcag cctggtgctg tcgggcggcg ccaccgaggc ggtgtcggtg
     4681 cagccgggcg accacgtcag tctgcgcgtg cagggcatgg gcagcgtgtc gctgcgcttc
     4741 gtctgaaact cagccccggg cgccggccag acaggcgccc atcgaccgac gcaccggaga
     4801 ccttcatgaa acgacgcgac ttcatcgccg cggcgagcgc cgccacgctg tgccacgcaa
     4861 gctgggcgca ggacgccttt ccagcccgcc cggtcaagct gatcgtgccg ttcccgccgg
     4921 gcgggccgac cgacatcatg ggtcgcaccg ccgccaaggc catgggcgac aggctgggcc
     4981 agcagttcgt ggtcgagaac aaggccggtg ccggtggcaa catcggcacc gacgccgtgg
     5041 ccaaggcggc gcccgacggc tacaccatcg gcctggcggc catcagcagc ctggccatcg
     5101 cgccgcacct gtacggcaag ctgccgttca acgtcgacaa ggacctggcg ccgatctcgc
     5161 tggtgggcac cacgccctgc gccatcatgg ccggtccgca ggcaccgttc tcggacctga
     5221 agggcctgat cgcctacgcc aaggccaacc ccggcaagct cagctacgcc acctcgggca
     5281 tcggaaccag caaccatctg gcggcggaac tgctgcagtc ggtcgccggc attcagttga
     5341 cgcatgtccc ctacaagggg tcgagccaga tcgtgcccga cctgatcgcc ggcacggtgc
     5401 cgatgagcat ggaaagctcg ctggccacca ccttgcagca tgtgcgctcg ggcaagctca
     5461 aggccattgc cgtcacctcg gcccggcgtg ccaaggccct gcccgaggta cccacggtgg
     5521 ccgagtccgg ctatgcgggg ttcgaagtgg agacctggtt cggtctgatc gccccggcgg
     5581 ccacgcctgc cgccatcgtg tcccgtctgc agggcgcctg ggccgaaggc tcgcgcgggg
     5641 ccgacgccca gagcgcgttc gacaacatct cggccaacct gcgcgtcacc acgccggccg
     5701 agttcggcgc cttcatccag gccgagcaca ggcgctgggg tgagctgatt cgcaagctgg
     5761 gcatcaaggc cgattgatgg ggggtgccgc gggccggcat gtgccagcgc gtcgcctggg
     5821 ccgcgcggcc tgcgcggcgc tgctgctgtc cctgggtgcg ttgcggggtg ctgccgagcc
     5881 cgcgcgggcg ctgggcggcg aagcagctgc cgccggaccg tcctggcacg cgcgtgtgga
     5941 gtttgtcgtg gatggcgatt cgctgtgggt gcggccggag cgcggcggtc ggcgcgctcg
     6001 gctgcgcctg cagggcatcg acgcgcccga gctgtgccag ccgggcggcg cgtcggcgcg
     6061 tgcggccctg caggcgctgg tgcgtggcga gcggctgcgc gtgaccgtgc atgcccggga
     6121 ccggttcggg cgtcgcctgg cgagcgtgca gcgccagcgc gacgggctgg atgtggcggc
     6181 gggcatggtg gcccagggct gggcctggaa cgaccgccag ggctggcgcc gcggccgcta
     6241 cgcacgcgag gaggcgcagg cccgcgcggc ccggcgcggc gtgttcgccg ccgccgatgc
     6301 ccagtcgccg gccgactttc ggcgccgcca cggaccgtgc gggaccgcgc agtctggtgc
     6361 cgcgtcgacg tccagatagc tacagaaata atagcttctg gcgctgatcc ggagcgccgt
     6421 tctacgccaa tcgatgccca acaccggcga ccggcgaccg gcgaccggcg accggcgacc
     6481 ggcgaccggc gaccggcgtc aaggcgcgag ctgcaaggac gagcgcagcg tctgggtctc
     6541 tgac
//
LOCUS       DAOOOG010000145         6540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_14047_length_6540_cov_24.844256, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000145 DAOOOG010000000
VERSION     DAOOOG010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14047_length_6540_cov_24.844256"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3620)
                     /gene="cobN"
                     /locus_tag="PK242_10350"
     CDS             complement(<1..3620)
                     /gene="cobN"
                     /locus_tag="PK242_10350"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HOZ94462.1"
                     /translation="MKHMRWLLCAALLLLVGVARAASVLFIATGNVPQGKFHQLAEIA
                     RPHGIEVEVRYLNSLPADVDAGLWRGRDAVFFDSYQQDEVRDRLVRALPGLAAPNAWL
                     YDARPAWGGGLPEAVARRLITYYASGGRQNYEGFFATLAAQLKGGDALAAAPEPVVFP
                     KTGIYHPRWPGLVTGDVHAYLRAQGVDRAAAGHKPVIAIALHQQYIGSMQTAYIDDMV
                     ARVEAGGAVALPFYTPMMGEGGFPKVLQPGGPGTPVLADLLINTQITLNADERRAEFE
                     RLGLPVIQAMTYRRGDEAEWAASQQGIATMDVPFYLAQAEYAGITDIQVASATRKGDE
                     QIMPITAQAAAVAAKALNLIKLQRKPNADKRVAVMFWNYPAGEKNLSASFLNVPRSLQ
                     TTLAAMAASGYRTEVPPDETHLITLLQRLLAPAYRGGELEPLLRDGLAALLPVKDYRA
                     WLAGLPRPTQEALASAWGAPEKSPWVLRQNGVDYFVIPRLELGHITLLPQPGRSGMAP
                     GSKDARAKEKEIYHSTTDLPPHHYLATYLWTRRHNDALVHYGTHGTQEWLPGKERGLS
                     VYDAPLLALGDIPVAYPYIADNIGEATQAKRRGRATIISHQTPPFAPGGLHEALTQMH
                     DLLHQWQAQDEGAVRERMALDLLAAAKKERVIADMGWTEARAKAQFADFVQELHNHLH
                     ELAQTAQPQGLHTLGRAPEELHRLGTVLLMLGSDFWEAAARHAGVPAADLDEALIGPW
                     DRLAQTVPYQLLKKHVVDGEATNDLPEQLQKALQKAAQVYADIGAQNELKGLLTVLDG
                     RYLATSYGGDPIKNPDAYPTGRNLYGFDPSRIPTKQAWEAGKQAAENMLAEHRKLHGR
                     QPKKLTFSLWSVETMRHFGMLEAQALWLLGVEPVWDQGGRVTGVKLVDRERLGRARVD
                     VVLSATGLYRDHFPNVMKQLAQAVLLASRARDEADNPVAANSERIAQRLIAQGADAKA
                     AELAGATRIFASESGRYGAGLDDAALATDTWEGKAEGDKKLAQLYLSKMQFAYGPDEK
                     QWGSAGVAGAAAKGGKAVNLYAEHLSGTEGAVLSRSSNLYGMLTTDDPFQYLGGIALA
                     VRHLDGKAPELYISNLRGAGSGKVEGAAQFLAKELATRQFHPGYIQGLMKEGYAGTLQ
                     VLDATNNFWGWTATAREIVRDDQWQEMVDVYVRDKHNLGLKDWFESKNPHALAQTMER
                     ML"
     gene            complement(3678..5633)
                     /locus_tag="PK242_10355"
     CDS             complement(3678..5633)
                     /locus_tag="PK242_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ94463.1"
                     /translation="MSTSLHPALSCRRHPLALAALLLVSHAATAQSTSAQSSTAALPA
                     VTVTATMTEQDARTAPASVTVITAEELAEKNATDLLDAVRGAPGITLQGRQVGGRKTL
                     ALRGLEGKHTLTLIDGRRISASDDVIGHSDYQYGWLPISAIERVEIIRGPMSALYGSE
                     ALGGVINLITKRPKDRWSGSIGVSGATGLSSDAASEGGTSIYAGGPLGERARLSVNAE
                     YAHRNATPDVDDPRYSEIEGRKPRNLGLNAEFDLTAQQRLQAGISDGKERRLYDDVST
                     AGKAYRNRYDLERRQTHLGWVGDFGDTKAQLRAYRSEMSIRNTRTNGVSPTRPQDMQD
                     DVVDGHVTMRRGGHQIAFGGEWRNEELVNAGLKGGRDDVTHKALFVQDEFALTRNLMA
                     TLGLRADHHGIFGSELSPRAYLVWEASPSLVVKGGYGHAFKAPTLKQISPNYVGAEGP
                     HTFMGNANIKPETSNSFEIGADWQVSPAWSLRATAFHTEVKQLITYRLLQQIGIRRIY
                     QYDNVDAARIQGLEAGFTWAITPQLSWSTDATVLHTRDKTTGKRLNDRPTTSVASHLA
                     WRVDGWDLRLGLQHTGSQESYGNKLPAYTLWNASVGRVWKLGGTQSLNVRAGLENMGN
                     VRLAEKSPNFGYAEQGRRVFLSARLDF"
     gene            5782..6522
                     /locus_tag="PK242_10360"
     CDS             5782..6522
                     /locus_tag="PK242_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ94464.1"
                     /translation="MTFVNARAHDASAQQPPRTSEVQRLLGNCRPLWGLPQAVLSEMA
                     GQASVARHADGEMLFYEGDPARHCLLVAQGAVALLRHDERGEERLWQCFGTGALVAEA
                     AMFLPGGLHPLSARAQGAVRVWQIPGQALRAACLAYPELALRMLQCLSQCLHQRVNEV
                     DWLTRGNTQERLASYVLAQAMQQGTCIELPCSQRHLAARLGVRAETLSRLLAQWQARG
                     WVQGRGRHWRLCDRGALQRLAPSGAHPF"
BASE COUNT          887 a         2182 c         2349 g         1122 t
ORIGIN      
        1 tcgagcatgc gctccatggt ctgcgccagc gcgtgcgggt tcttggactc gaaccagtcc
       61 ttgaggccga ggttgtgctt gtcgcgcacg tagacgtcga ccatctcctg ccactggtcg
      121 tcgcgcacga tttcgcgcgc ggtggccgtc cagccccaga agttgttggt ggcgtccagc
      181 acctgcagcg tgcccgcgta gccctccttc atcaggccct ggatgtagcc ggggtggaac
      241 tggcgcgtgg ccagctcctt ggcgaggaac tgcgccgcac cctcgacctt gccactaccc
      301 gcgccgcgca ggttgctgat gtagagctcg ggcgccttgc cgtcgaggtg ccgcacggcc
      361 agcgcaatgc cgcccaggta ttggaagggg tcgtcggtcg tcaacatgcc gtagaggttg
      421 gagctgcgcg agagcaccgc gccctcggtg ccgctcaggt gctcggcgta gaggttgacg
      481 gccttgccgc ccttggctgc cgcccccgcc acgccggcgc tgccccattg cttctcgtcc
      541 ggcccatagg cgaactgcat cttggacaga tacagctggg cgagcttctt gtcgccctcg
      601 gccttgccct cccaggtgtc ggtggcgagc gccgcgtcgt ccagcccggc gccgtagcgc
      661 ccgctctccg aggcaaagat gcgcgtggcg ccggcgagct ccgccgcctt ggcatcggcg
      721 ccctgggcga tgagtctttg cgcgatgcgc tcgctgttgg cggccacggg gttgtcggcc
      781 tcgtcccggg cgcgggacgc cagcagcacc gcctgggcca gctgcttcat gacgttgggg
      841 aaatggtcgc ggtacaggcc ggtggccgac agcaccacgt ccacgcgcgc gcggcccagc
      901 cgctcgcggt ccaccagctt cacgccggtg acgcgcccgc cctggtccca cacgggctcg
      961 acgccgagca gccacagggc ctgggcctcg agcatgccga agtggcgcat ggtctccacc
     1021 gaccagaggc tgaaggtgag cttcttcggc tgcctgccgt gcagcttgcg gtgctcggcc
     1081 agcatgttct cggccgcctg cttgccggcc tcccaggcct gtttggtggg gatgcgcgag
     1141 gggtcgaagc catagaggtt gcggcccgtg ggataggcgt cggggttctt gatcgggtcg
     1201 ccgccgtagg aggtagccag gtagcgcccg tccagcaccg tgagcaggcc cttgagctcg
     1261 ttctgcgcgc cgatgtcggc atagacctgc gccgccttct gcaatgcttt ctgtagctgc
     1321 tcgggcaggt cgttcgtggc ctcgccatcg accacatgct ttttcagcaa ttggtagggc
     1381 acggtctgcg ccagcctgtc ccaggggccg atcagggcct cgtccaggtc ggctgcgggc
     1441 acgcccgcgt ggcgcgccgc ggcctcccag aagtcgctgc ccagcatgag cagcacggtg
     1501 cccaggcggt gcagctcctc gggcgcgcgg cccagggtgt gcaggccctg gggctgggcg
     1561 gtctgggcca gctcgtgcag atggttgtgc agctcctgca cgaagtcggc gaactgcgcc
     1621 ttggcgcgcg cctcggtcca gcccatgtcg gcgatcacgc gctccttctt cgccgcggcc
     1681 agcagatcca acgccatgcg ctcgcgcacc gcgccctcgt cctgcgcctg ccactggtgc
     1741 agcaggtcat gcatctgggt cagggcctcg tgcaggccgc cgggggcgaa cggcggggtc
     1801 tggtggctga tgatggtggc gcgcccgcgg cgcttggcct gggtggcctc gccgatgttg
     1861 tcggcgatgt aggggtaggc cacgggaatg tcgcccagcg ccagcagcgg cgcgtcgtac
     1921 accgacaggc cgcgctcctt gcccggcagc cattcctgcg tgccatgcgt gccgtagtgc
     1981 accagcgcgt cgttgtgccg gcgcgtccac agataggtgg ccaggtagtg gtgcggcggc
     2041 aggtcggtgg tggagtggta gatctccttt tccttggcgc gcgcgtcctt gctgcccggc
     2101 gccatgccgc tgcggccggg ctggggcagc agcgtgatgt ggccgagctc aaggcgcgga
     2161 atcacgaagt agtccacgcc gttctggcgc agcacccagg gggatttctc gggcgcgccc
     2221 caggcgctcg ccagcgcctc ctgcgtgggg cgcggcaggc cggcgagcca ggcgcggtag
     2281 tccttgacgg gcagcagcgc ggccaggccg tcgcgcaaga gcggctcaag ctcgccgccg
     2341 cggtaggccg gggccagcag gcgctgcagc agggtgatca gatgcgtctc gtcgggcggc
     2401 acctcggttc ggtagcccga ggccgccatc gcggccagcg tggtctgcag gctgcgcggc
     2461 acgttgagga acgaggccga gaggttcttc tcgcccgcgg ggtagttcca gaacatcacg
     2521 gccacgcgct tgtcggcgtt gggcttgcgc tgcagcttga tcaggttcag cgccttggcg
     2581 gccacggccg cggcctgggc cgtgatgggc atgatctgct cgtcgccctt gcgcgtggca
     2641 gacgccacct gaatgtcggt gatgcccgcg tactcggcct gggccaggta gaagggcacg
     2701 tccatggtgg caatgccctg ctggctcgcg gcccactcgg cctcgtcgcc gcggcggtag
     2761 gtcatggcct ggatgacggg cagccctaag cgctcgaact cggcgcggcg ctcgtcggcg
     2821 ttgagcgtga tctgggtgtt gatgagcagg tcggccagca cgggcgtgcc gggcccgccg
     2881 ggttgcagta ccttggggaa ccccccctcg cccatcatgg gcgtgtagaa cggcagggcc
     2941 acggcgccgc cggcctccac gcgcgcgacc atgtcgtcga tgtaggccgt ctgcatcgag
     3001 cctatgtact gctggtgcag cgcaatggcg atgacgggct tgtgccccgc ggccgcccgg
     3061 tctacaccct gggcgcgcag ataggcgtgc acgtcgcccg tcaccaggcc gggccagcgc
     3121 gggtggtaga tgccggtctt ggggaagacc acgggctcgg gcgccgcggc cagcgcgtcg
     3181 ccgcccttga gctgcgccgc cagcgtggcg aagaagccct cgtagttctg gcgcccgccg
     3241 ctcgcgtaat aggtgatgag gcggcgcgcc acggcctcgg gtaggccccc gccccaggcc
     3301 gggcgtgcgt cgtacagcca ggcattgggc gcggccaggc cgggcagcgc gcgcaccagg
     3361 cggtcgcgca cctcgtcctg ctggtagctg tcgaagaaca ccgcgtcacg cccgcgccac
     3421 aggccggcat cgacgtcggc cggcaggctg ttcaggtaac gcacctcgac ctcgatgcca
     3481 tgggggcggg caatctcggc cagctggtgg aacttgccct gcggcacgtt gcccgtggcg
     3541 atgaacaaca ccgacgcggc acgcgccacg ccgaccagca ggagcagcgc ggcgcacagc
     3601 agccagcgca tgtgtttcat agtcaaattg gcctcctgcg ccctatgggc gcgcgccagc
     3661 agctactgtt tctatagtca gaaatccagc cgcgccgaca ggaacacgcg ccgcccctgc
     3721 tcggcgtagc cgaagttggg cgacttctcg gccaggcgca cattgcccat gttctccagg
     3781 ccggcgcgga cgttgagact ttgcgtgccg cccagtttcc agacccggcc cacgctggcg
     3841 ttccacaagg tgtaagcggg cagtttgttg ccgtagcttt cctggctgcc ggtgtgttgc
     3901 aggcccaggc gcagatccca gccgtcgacg cgccaggcca ggtgcgaggc cacgctggtc
     3961 gtggggcggt cgttcaggcg cttgccggtc gtcttgtcac gcgtgtgcag cacggtggcg
     4021 tcggtgctcc aggacagctg cggcgtgatg gcccaggtga agcccgcctc caggccctgg
     4081 atgcgcgcgg cgtccacgtt gtcgtactgg tagatgcggc gtatgccgat ctgctgcagc
     4141 aggcggtagg tgatgagctg cttgacctcg gtatggaagg ccgtggcgcg cagcgaccag
     4201 gcggggctga cctgccagtc ggcgccaatc tcgaaggagt tcgatgtttc tggcttgatg
     4261 ttggcattgc ccatgaaggt atgcggcccc tcggcgccca cgtagttggg tgaaatctgc
     4321 ttgagcgtgg gcgccttgaa ggcgtggcca tagccgccct tgacgaccag actcgggctg
     4381 gcctcccaga ccaggtaggc gcgcgggctg agctcggagc cgaagatgcc gtgatggtcg
     4441 gcgcgcaggc ccagcgtggc catgaggttg cgcgtgagcg cgaactcgtc ctgcacgaac
     4501 agcgccttgt gcgtcacgtc gtcgcgcccg cccttgaggc cggcgttgac cagttcctcg
     4561 ttgcgccatt cgccgccaaa ggcgatctgg tggccgccgc ggcgcatggt cacatgaccg
     4621 tcgaccacgt cgtcctgcat gtcctgcggc cgcgtgggcg acacgccatt ggtgcgcgtg
     4681 ttcctgatgc tcatctcgct gcggtaggcg cgcagctgcg ccttggtatc tccaaagtcg
     4741 cccacccagc ccaaatgggt ctggcgccgc tccaggtcgt agcggttgcg gtaggccttg
     4801 ccggccgtgc tcacgtcgtc gtacaggcgc ctttccttgc cgtcgctgat gccggcctgc
     4861 aggcgctgct gggccgtcag gtcgaactcg gcgttcagcc ccaggttgcg cggcttgcga
     4921 ccctcgatct cgctgtagcg cgggtcatcc acgtcgggcg tggcgttgcg gtgggcgtat
     4981 tcggcgttca ccgacaggcg cgcgcgctcg cccagcgggc cgcccgcgta gatcgaggtg
     5041 ccgccctcgc tcgccgcgtc gctggacagc cccgtcgcgc ccgagacgcc gatggagccg
     5101 ctccagcggt ccttgggccg cttggtgatg aggttgatca cgccgcccag ggcctcggaa
     5161 ccgtacagcg ccgacatcgg gccgcggatg atctccacgc gctcgatcgc cgagatcggc
     5221 agccagccgt actggtagtc ggaatggccg atcacgtcgt cgctggcgct gatgcgccgc
     5281 ccatcgatca gcgtcagcgt gtgcttgccc tccaggccac gcagcgccag cgtcttgcgc
     5341 ccgcccacct ggcgcccctg cagcgtgatg ccgggcgcgc cgcgcacggc gtccagcagg
     5401 tcggtggcgt ttttctcggc cagctcctcg gccgtgatga cggtgacgct ggccggcgcg
     5461 gtgcgcgcgt cctgctcggt catggtggcc gtcacggtga cggccggcag ggcagcagtg
     5521 gaggactggg cagaggtcga ctgggccgtg gccgcgtggc tgaccaacag aagagcggcc
     5581 agagccaggg gatggcgacg gcacgacagg gcgggatgca gggaggttga catgggcgtc
     5641 caaggattga ccgcggtggc tggacgcatc ggcccgtcgg cgcgcgttgg gctgggcggg
     5701 aaaaaagaaa agggattaag atagaaacgc ggatacgaat gattcgcatt cgtaattatt
     5761 ctcatggcag cccggaatca aatgacattt gtcaatgcgc gcgcccatga tgcgtcggcg
     5821 caacagcctc cgcgcaccag cgaggtgcag cgcttgctcg gcaactgccg gcccttgtgg
     5881 ggcttgccgc aggcggtgct gagtgaaatg gccggtcaag ccagcgtcgc tcggcacgcc
     5941 gacggcgaga tgctgttcta cgaaggcgac ccggcacggc actgcctgct ggtggcgcaa
     6001 ggtgcggtgg cgctgctgcg ccatgacgaa cgcggcgagg aacgcctttg gcagtgcttc
     6061 ggcaccggcg cgttggtggc cgaagcggcg atgtttctgc ccggtggcct tcaccccctg
     6121 tcggcgcgcg cgcagggcgc ggttcgggtc tggcagattc cgggtcaggc gcttcgagcg
     6181 gcatgtcttg cgtacccgga acttgcgctg cgcatgctgc agtgcctgag ccaatgcctg
     6241 caccagcgcg tcaacgaagt cgattggctg acccgtggca acacccagga gcgcctggcc
     6301 agctacgtgc tggcgcaggc catgcagcag ggcacctgca tcgaactgcc ttgcagccag
     6361 cgccatctgg cggcccggtt gggcgtgcgc gccgagacgc tcagccgcct gcttgcgcaa
     6421 tggcaggcgc gcggctgggt gcagggcaga gggcgccatt ggcggctgtg cgaccgtggt
     6481 gcgctgcagc gcctggcacc atcgggcgcg cacccgttct gacgcctgaa gtggcagatt
//
LOCUS       DAOOOG010000146         6511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_14145_length_6511_cov_27.864312, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000146 DAOOOG010000000
VERSION     DAOOOG010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14145_length_6511_cov_27.864312"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PK242_10365"
     CDS             <1..346
                     /locus_tag="PK242_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ94465.1"
                     /translation="WEALADGLLDAAILARLEKTWPGRAEGERSPAWIARQMKKIDDA
                     LAAMSAGLADKPWCSAGVHLSLADIAVGCALGYLDFRFPELDWRERHANLARLLDERL
                     MPRPSFADTVPA"
     gene            complement(638..2275)
                     /locus_tag="PK242_10370"
     CDS             complement(638..2275)
                     /locus_tag="PK242_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ94466.1"
                     /translation="MSPGWRRRNWLAAGAGALLGAACTRDAAQAIEGGFANDSDARGH
                     RLRQPWPTRAPDLTRHVHTVIAGGGIAGLAAARALRLAGHDDFALLELEDTAGGHSRG
                     MQVNGIACPMGAHYLPLPGDDARAVQDLLEELGLRRRVAGRWQYDERALCHSPQERLF
                     LHGQWQDGLLPLQGVGGATLAQYRRFAAAVASAQAAAHYAIPVQKSSFSPVQLAQDAI
                     TFEAWLNQQGFHDRQLRWYLDYCCRDDYGAGSASVSAWAGLHYFASRHGFAAPGDAAH
                     DDAGVLTWPEGNGWLAAQLAAPLAERLQPARVVGRIEHGRHGVTLDALDIRSGRLERW
                     QARQAVIALPVWVAARVVEASPEALRQRAARLRSAPWLLAQLKLRAPLRERPGAAPSW
                     DNVLYGAPGLGYVNAMHQSLHPVPGATVLTYYRALGDAPDGRQRLLDTPWTAWRDAIL
                     AELGAAHPDLAERTERMALLRHGHAMAVPTPGTLSQIERLRSSRKRQQLSNAEHAGWV
                     FDAAPRLAFAHADWSGYSVFEEAFTLGHAAGTALAVR"
     gene            complement(2272..3882)
                     /locus_tag="PK242_10375"
     CDS             complement(2272..3882)
                     /locus_tag="PK242_10375"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOZ94467.1"
                     /translation="MTASSSTLVAPTGAAPRPMAVALLASVFVIAACGLLYELAAGAL
                     ASYLLGDSVLQFSTVIGTYLFAMGVGSWLSRYFERQLPAHFLRIELLIALFGGALPAL
                     LFVANATVPGAFRPLLYGMVLVVGTLVGLEIPLVMRILKRDVTIRDLVSQVLTFDYLG
                     ALAVSLAFPLVLVPRLGLIRTGFLFGLMNAAVAIWALWLFRHGLPRRRAFAVASAVVV
                     ALLAAGMAGAGRITSLAEEHFYQAPITFAESTPYQRIVVTGGHGGLRLFLNGNLQFAQ
                     RDEYRYHEALVHPAMAAHGAPRRVAVLGGGDGMAVREVLKYPSVESVTLVELDPAMTQ
                     LFRDTPQLAQLNHGALRDPRVQIVNTDAFQWLQQGAAPTAQEPAAADPLAARGGEAFY
                     DVIVVDFPDPTNFSIGKLYTLSFYALLEQRLAASGYAVIQTTSPLVARRSFWTVVQTV
                     EAAGLSATPYHAHVPSFGEWGFIVAGRRPWRLPGTLPDGLRFLTPHTLPLLFDFPADM
                     ARVPAEVNRLSNQALVHTYETEWGRVQE"
     gene            complement(3938..4156)
                     /locus_tag="PK242_10380"
     CDS             complement(3938..4156)
                     /locus_tag="PK242_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HOZ94468.1"
                     /translation="MDAEWLKPAALIGSLVYALIGVAIFWLCFVIIDKLTPVNLWQEI
                     VEKHNRALALVVAAMCLGISIIVAAAIH"
     gene            complement(4184..5680)
                     /locus_tag="PK242_10385"
     CDS             complement(4184..5680)
                     /locus_tag="PK242_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HOZ94469.1"
                     /translation="MATDAPQRHYRAACPSCGAPVEFQSATAPFAVCGFCRSTVVRSG
                     EQLQRIGQMAELFDDHSPLQLMASGRVTLDGNKLPFTLIGRLQFKSDSGTWTEWIAAL
                     DDGRTGTLSEDNGSYVFTRPDEGTEPPPAQPLPAAGSSLRLGVTDWVVAYSGSAQLLA
                     AEGELPKLPALGQPFKLLELRSEDGQVLSIDYGAAPPQVERGRAVELAHLQLKGLKDA
                     SVREDQGGRSFACPNCGAPVHVQLDNTKRCTCAQCESLIDLTAGVGGELLAAKQKVRV
                     PPLIPLGSRGQLQGTSWQVVGWQRRSGREPGDDEKFEWKEYLLYHRERGFAFLVDTED
                     GWSLVFTATGAPRIDQGKRRARYQGSTYTLQSAYEATTTEVLGEFYWPVERGQKTDNH
                     DYANGNKLLSTERSNGEVTASVGSRIDGAAVAEAFGMSQRKALFERAVIGPAELAAEG
                     MTQKIIMAIVVIIVISTVLDSCSLELDDYLARGTGGAFGGYSSGGSHK"
     gene            complement(5747..>6511)
                     /locus_tag="PK242_10390"
     CDS             complement(5747..>6511)
                     /locus_tag="PK242_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOZ94470.1"
                     /translation="SPFKSDVYFFSTRQQVDQKWGTPQPISIRDKEFGAVRLRAFGNY
                     AYRVADPKKFHTEISGTRDVYTVDDLDGQLRGLITQHIATGIAQSGIPFLDLAANQQA
                     FAQALSNALAPAMEAYGLKLEGMTVQSISLPEELQKVLDQRIGMGMVGGDMGKFMQYQ
                     TAQAIPKMAEGVGAGGDAGGLAGAGLGMGAGVAMGQILAQNMQSALQGGATPAAGVRP
                     EDVMATLEKLGELKAKGILTEDEFNAKKAELLKKLV"
BASE COUNT          976 a         2253 c         2329 g          953 t
ORIGIN      
        1 ctgggaggcg ctggccgacg gcctgctgga cgccgccatc ctggcgcggc tggagaaaac
       61 ctggcccggc cgcgccgagg gcgagcgcag cccggcctgg atcgcgcggc agatgaagaa
      121 gatcgacgat gcgctggccg ccatgagcgc cggcctggcc gacaagccct ggtgttcggc
      181 cggcgtgcac ctgtcgctgg ccgacatcgc cgtgggctgc gcgctgggct acctggactt
      241 tcgctttccc gagctggact ggcgtgagcg ccacgccaac ctggcgcgcc tgctggacga
      301 gcgcctgatg ccgcgcccca gctttgccga caccgtgccg gcataggcag cgcgcccaga
      361 tgaacgcgcc gccggggcac ggggcccggg cggcgcggtg ggaataaaaa agctgccgaa
      421 gcggcctgaa acgaaagagg aggagggagg aggaggaagt ccgcctcagg ttttcagccg
      481 actcgcggag ccggcaggct cggcagcgtc aaggcaattc atggagtcaa tgccatgcat
      541 cacgtggatt gagacgtgag tgtgcgccca aagttccgcc cgcgctgccc cggcgcgcgt
      601 aaagaagtgt gaaagcctgg ggcctgcgca cagaccctca gcgcaccgcc agcgcggtgc
      661 cggcggcgtg gcccagggtg aaggcctctt cgaacaccga gtagcccgac caatcggcat
      721 gggcaaaggc cagccgcggt gcggcatcga aaacccatcc cgcgtgctct gcatttgata
      781 gctgctggcg ctttctggac gagcgcagac gctcgatttg gctcaaagta ccgggggtgg
      841 gcacggccat ggcgtgaccg tggcgcagca gcgccatgcg ttcggtgcgc tccgccaggt
      901 cggggtgggc cgcgcccagc tcggccagga tggcgtcgcg ccaggcggtc cagggcgtat
      961 cgagcaggcg ctggcggccg tcgggtgcgt cgcccagtgc gcggtagtag gtcagcaccg
     1021 tggcgccggg caccgggtgc aggctttggt gcatggcgtt gacgtagccc aggccgggcg
     1081 cgccgtacag cacgttgtcc cagctgggcg cggcaccggg gcgctcgcgc agcggtgcgc
     1141 gcagcttgag ctgggccagc agccaggggg cgctgcgcag gcgggcggcg cgctggcgca
     1201 gggcttcggg cgacgcttcg acgacgcgcg cggccaccca caccggcagg gcgatcacgg
     1261 cctggcgcgc ctgccagcgc tccagccggc cgctgcggat gtcgagcgcg tccagcgtca
     1321 cgccgtggcg cccgtgttcg atgcggccga ccacgcgcgc cggctgcagg cgctcagcca
     1381 gcggcgccgc cagctgcgcc gccagccagc cgttgccctc gggccaggtc agcacgccgg
     1441 cgtcgtcgtg cgccgcgtcg ccaggcgcgg caaagccgtg ccggctggca aagtagtgca
     1501 gcccggccca ggccgacacg ctggcgctgc cggcaccgta gtcgtcgcgg cagcagtagt
     1561 cgaggtacca gcgcagctgg cggtcatgaa acccctgctg gttcagccat gcttcaaacg
     1621 taatagcgtc ttgcgcaagc tggacgggcg aaaacgacga tttctgcact ggaatggcgt
     1681 agtgcgcggc ggcctgcgcg ctggccaccg cggcggcaaa gcgccggtac tgcgccagcg
     1741 tggcgccgcc cacgccctgc agcggcagca gaccgtcctg ccactggccg tgcaggaaca
     1801 ggcgctcctg cgggctgtgg cacagggcgc gttcgtcgta ctgccagcgc ccggccacgc
     1861 gccggcgcag gcccagctct tccagcaggt cctgcaccgc gcgggcatcg tcgccgggca
     1921 gcggcaggta gtgcgcgccc atcgggcagg cgatgccgtt cacctgcatg ccgcggctgt
     1981 ggccgccggc cgtgtcttcc agctccagca gggcgaagtc gtcgtgcccg gccaggcgca
     2041 gcgcgcgcgc cgccgccagc ccggcgatgc cgccgccggc gatcacggtg tgcacgtgcc
     2101 gcgtgaggtc gggcgcgcgc gtgggccagg gctggcgcag ccggtggccg cgcgcgtcgc
     2161 tgtcgttggc aaaaccgcct tcgatggcct gcgccgcgtc gcgcgtgcag gccgcgccca
     2221 gcagtgcgcc ggcgccggcc gccagccagt tgcggcggcg ccagccgggg ctcattcctg
     2281 cacccgtccc cattcggttt cgtaggtgtg caccagcgcc tggttggaca ggcggttgac
     2341 ctcggccggc acgcgcgcca tgtcggccgg aaagtcgaac agcagcggca gcgtgtgcgg
     2401 cgtcagaaaa cgcaggccgt cgggcagggt gccgggcagg cgccagggcc tgcggccggc
     2461 gacgatgaac ccccactctc cgaagctggg cacatgggcg tggtaggggg tggcgctcag
     2521 gcccgccgct tcgaccgtct gcaccacggt ccagaagctg cgccgcgcca ccagcggcga
     2581 cgtggtctgg atcaccgcgt agccgctggc ggccaggcgc tgctccagca gggcgtagaa
     2641 gctgagcgtg tagagcttgc cgatgctgaa gttggtgggg tcgggaaagt ccaccacgat
     2701 gacgtcatag aaggcttcgc ccccacgtgc cgccagcggg tcggcggcgg cgggttcctg
     2761 ggcggtgggc gctgcgccct gctgcagcca ctgaaaggcg tcggtgttga cgatctgcac
     2821 ccgggggtcg cgcagcgccc cgtggttgag ctgcgccagc tgcggcgtgt cgcgaaacag
     2881 ctgcgtcatg gccgggtcca gctcgaccag ggtgaccgat tcgaccgagg ggtacttgag
     2941 cacctcgcgc accgccatgc cgtcgccgcc acccagcacg gcgacccggc gcggtgcgcc
     3001 atgcgccgcc atcgccgggt gcaccagggc ctcgtggtag cggtactcgt cgcgctgggc
     3061 gaactgcagg ttgccgttca gaaacagccg caggccgccg tgcccgccgg tcaccacgat
     3121 gcgctggtag ggcgtggatt cggcaaaggt gatgggggcc tggtagaagt gctcttcggc
     3181 caggctggtg atgcgcccgg cacccgccat gccggccgcc agcagcgcca ccaccaccgc
     3241 actggccacg gcaaatgcgc gccggcgcgg caggccatgg cgaaacagcc acagcgccca
     3301 gatcgccacc gccgcgttca tcaggccgaa cagaaagccg gtgcggatca gccccagacg
     3361 cggcaccagc accagcggaa aggccagcga caccgccagc gcgccgaggt agtcgaaggt
     3421 cagcacctgg ctgaccaggt cccggatcgt cacgtcgcgc ttgaggatgc gcatgaccag
     3481 cgggatttcc agccccacca gcgtgcccac caccagcacc atgccgtaca gcagcggccg
     3541 aaacgccccc ggcacggtgg cgttggcgac gaacagcagc gccggcagcg cgccgccgaa
     3601 cagcgcgatc agcagctcga tgcgcagaaa gtgcgccggc aactggcgct cgaaatagcg
     3661 cgacagccac gagcccacgc ccatggcgaa caggtaggtg ccgatgacgg tggaaaactg
     3721 cagcaccgaa tcgcccagca gatagctggc cagcgcgccc gccgccagtt cgtacagcag
     3781 gccgcaggcg gcgatgacga acacgctggc cagcaacgcc acggccatcg gacggggggc
     3841 ggcgcccgtg ggcgcgacca gggtggacga agatgccgtc atgacagggg ggcgcggcca
     3901 cgcccggcgc tgcggcgcgc cggcgcgtgc cgcggcgtca atggatggcc gccgccacga
     3961 tgatgctgat gcccagacac atggcggcca ccaccagggc cagcgcgcgg ttgtgctttt
     4021 cgacgatttc ctgccacagg ttgacgggcg tgagcttgtc gatgatgacg aagcacagcc
     4081 agaagatcgc caccccgatc agggcgtaga cgagcgagcc gatcagggcg gcgggtttga
     4141 gccattcagc gtccatggtg atgacctccg tgggtggata acctcatttg tggctgccgc
     4201 cgctcgagta gccgccaaag gcgccgccgg tgccgcgcgc caggtagtcg tcgagctcaa
     4261 gcgagcagct gtcgagcacc gtcgagatga cgatgatgac gacgatggcc atgatgatct
     4321 tctgcgtcat gccctccgcg gccagctcgg ccggcccgat cacggcgcgc tcgaacagcg
     4381 ccttgcgttg gctcatgcca aaggcctcgg ccaccgccgc accgtcgatg cggctgccga
     4441 ccgaggccgt gacctcgccg ttgctgcgct cggtggacag cagcttgttg ccgttggcgt
     4501 agtcgtggtt gtcggtcttc tggccgcgct cgaccggcca gtagaactcg cccagcacct
     4561 cggtggtggt ggcttcgtag gccgactgca gggtgtaggt gctgccctgg tagcgcgcgc
     4621 gccgcttgcc ctggtcgatg cgcggcgcgc cggtggcggt gaacaccagg ctccagccgt
     4681 cttcggtgtc gaccagaaag gcaaagccgc gctcgcggtg gtagagcagg tactccttcc
     4741 actcgaactt ctcgtcgtcg cccggctcgc gcccgctgcg gcgctgccag cccaccacct
     4801 gccagctcgt gccctgcagc tggccgcggc tgcccagggg gatgagcggc ggcacgcgca
     4861 ctttctgctt ggccgccagc agctcgccgc cgacgccggc cgtcagatcg atcaggctct
     4921 cgcactgcgc gcaggtgcag cgcttggtgt tgtcgagttg cacatgcacc ggcgcgccgc
     4981 agttggggca ggcgaaggag cgcccgccct ggtcctcgcg caccgatgcg tccttcaggc
     5041 ccttgagctg caagtgcgcc agttccaccg cgcggccgcg ctcgacctgc ggcggcgcgg
     5101 cgccgtagtc gatggacagc acctggccgt cttcactgcg cagctccagc agcttgaagg
     5161 gctggcccag cgcgggcagc ttgggcagct cgccctcggc ggcgagcagc tgggcgctgc
     5221 cgctgtacgc caccacccag tcggtgacgc ccaggcgcag gctgctgccg gcggccggca
     5281 gcggctgggc cggcggcggc tcggtgccct cgtcgggccg ggtgaagacg tagctgccgt
     5341 tgtcttcgct cagcgtgccg gtgcggccat cgtccagcgc ggcgatccac tcggtccagg
     5401 tgccggaatc gctcttgaat tgcaggcgcc cgatcagggt gaagggcagc ttgttgccgt
     5461 ccagcgtgac gcgcccgctg gccatcagct gcagcgggct gtggtcgtcg aacagctcgg
     5521 ccatctggcc gatgcgctga agctgctcac cgctgcgcac caccgtgctg cggcagaagc
     5581 cgcacaccgc aaagggcgcg gtggcgctct ggaactccac cggcgcgccg caggacgggc
     5641 aggcggcgcg gtagtgacgc tgcggtgcgt cggtggccat gttatgggtg ttttatggct
     5701 ccggcgcgca tccaacaagc gccaacagct atcaaataag tagcaatcag accagtttct
     5761 tgagcagctc ggccttcttg gcgttgaact cgtcctcggt caggatgccc ttggccttga
     5821 gctcgcccag cttctccagc gtggccatca cgtcttcggg cctgacgccc gcggccggcg
     5881 tggcgccgcc ctgcagcgcc gactgcatgt tctgcgccag gatctgcccc atggccacgc
     5941 ccgcgcccat gcccaggccg gcgccggcca gaccgcccgc gtcgccgccg gcgcccacgc
     6001 cctcggccat cttgggaatg gcctgcgcgg tttggtactg catgaacttg cccatgtcgc
     6061 cgccgaccat gcccatgccg atgcgctggt ccagcacttt ctgcagctct tcgggcagcg
     6121 aaatgctctg caccgtcatg ccttccagct tgaggccgta ggcttccatc gccggtgcca
     6181 gcgcattcga cagcgcctgg gcaaaggcct gctggttggc cgccaggtcc agaaacggga
     6241 tgccgctttg cgcgatgccg gtggcgatgt gctgcgtgat cagcccgcgc agctggccgt
     6301 ccaggtcatc caccgtatag acatcgcgcg tgccggagat ctcggtgtgg aacttcttgg
     6361 ggtcggccac ccgataggcg tagttgccga aggcgcgcag gcgcaccgcg ccgaattcct
     6421 tgtcgcggat gctgatcggc tggggcgtgc cccacttctg gtcgacctgc tggcgcgtgc
     6481 tgaagaagta gacgtcgctc ttgaagggcg a
//
LOCUS       DAOOOG010000147         6485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_14211_length_6485_cov_24.588180, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000147 DAOOOG010000000
VERSION     DAOOOG010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14211_length_6485_cov_24.588180"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..2346
                     /locus_tag="PK242_10395"
     CDS             91..2346
                     /locus_tag="PK242_10395"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ94471.1"
                     /translation="MSKQQAHPSPSSAANGVPALFDRAVRLFNGGQPDQAAPLCQQVL
                     QQQPNHAHAWQLLGMVHVVGNRPAEALPALQRALALNPRQTQILCLLGTVLARLGQSE
                     QAIDHFRQALALAPQDANIWYDLGNVQHGLGQHLQALASHDRALALAPGHADAWLNRG
                     HALLELHRLGDAIASFERAIDLRPRASTWLLLGSALEQAQRLPDALACFEKVVALEPD
                     NLDGWLKFGGVLLQLGRPDLALERIEVAQRLAPQSPRVLLTQAAALAKTRGDAAALMP
                     LLQAHALAPDDLSVSMQLLYFMLKTAHWEGLTEVLAQTLRHWRAGARDANTLTLLAHP
                     DVSAADLLQIAQAYHAPLNDAFRAMHAKVARGAPAGRRLRLGYLSSDLRHHAVGFLMT
                     GVFEAHDRQRFETFAFSIYPQPDRSPERQRAQAAFDHFIDIHHLGDDEAAALIAGHGI
                     DLLVDLNGLTTFSRPGILARRPAPVQVQYLGFPGTSGLAAIDYILGDRWVTPVEQAQA
                     FSEHIVRMPDCFQANDDQRRFAAATPTRRELGLPDQGFVFCCLNNSYKILPGVFDGWM
                     RLLQNTPGSVLWLLGDNDTARSNLRAHAQARGVAPERLVFAERRPYDQYLARYRQADL
                     FLDTLPFNGGTTVSDALWAGLPVLTQVGDRFAGRMAASLLDCIGLPELITHSQPEYEA
                     LAHRLATEPGLLQGLRDRLAANIRTRPLFDTRRFTRHLERAFEHMIERHAQGLPPQGF
                     DVAELPLAQSA"
     gene            complement(2425..3381)
                     /locus_tag="PK242_10400"
     CDS             complement(2425..3381)
                     /locus_tag="PK242_10400"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOZ94472.1"
                     /translation="MENEVYVNGQPVPWERATIEKLLFAELRERRAARRWRTLRTVLW
                     LGLIGALAWLLWSDHLPSATPSSAHTAMVSIKGEISDTGEGSAEYILPALRSAMEDKG
                     AQALVLLINSPGGSPVQAGLIHDEVRRLRALHDKPVYAVVEESCASAAYYIAAAADRI
                     YVDKASLVGSIGVLIDGFGAVGALEKLGVERRLLTAGENKGFLDPFSPQSEAHRAHAQ
                     RMLDQIHQQFIDVVRQGRGERLKETPDMFSGLFWTGEQAVQLGLADQLGSLDMVAREV
                     VKAEKLVDYTNKQNFAERFAKRLGAQVGEGALAALRGAPGWR"
     gene            complement(3440..3817)
                     /locus_tag="PK242_10405"
     CDS             complement(3440..3817)
                     /locus_tag="PK242_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOZ94473.1"
                     /translation="MDNDLSPVYLCDSADLQEGGLAVPFDVAHGGENLRAFAVRFEGR
                     VHAYLNRCAHVPMEMDWQPNRFFDDSGQWLLCATHGATYAPDTGHCVAGPCSGAQLVR
                     IELIEAAGQVHWQPTGALRPIAF"
     gene            complement(3862..4524)
                     /locus_tag="PK242_10410"
     CDS             complement(3862..4524)
                     /locus_tag="PK242_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOZ94474.1"
                     /translation="MSRPRQFDLICFDWDGTLFDSTAIIVRSIQQAVLDVGGARPSDA
                     AAAYVIGMALVPALARAAPDVPADKYPELAARYRHHYLARQHDISLFEGVLPMLAELR
                     ARHHWLSVATGKSRRGLDEVLASRQLQGVFDGSRTADETAGKPHPRMLHELMREFGVE
                     PQRTLMIGDTTHDLQMALNAGCASVGVSYGAHAPDDFEALRPRQVAHSVAELHRWLSD
                     NA"
     gene            complement(4521..5528)
                     /locus_tag="PK242_10415"
     CDS             complement(4521..5528)
                     /locus_tag="PK242_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOZ94475.1"
                     /translation="MNRIIGAQAAPTVPAAKPAVLRLTVDADSGGQRLDNYLLRHLKG
                     VPKTHVYRIIRSGEVRLNKGRAAADTRVSEGDEVRVPPVRVAAPVQHLDGRAAPAREF
                     PVLLEDEQLIAIDKPAGVAVHGGSGVSFGVIEQLRQARGYGQPGGLRFLELVHRLDRE
                     TSGILLLAKKRSALKHLQDQFRERQTGKTYLALVLGEWPAARRVIDAPLHKYLLADGE
                     RRVRVVAPDDPDGMRAITLVKVRQRLAALTLLEVTIKTGRTHQIRVHLAAQGHPIAGD
                     DKYGDFERNKALQKQGLRRMFLHAWRLQFSHPERAERIELQAALPPDLLSFTKHLAPA
                     P"
     gene            6127..>6485
                     /locus_tag="PK242_10420"
     CDS             6127..>6485
                     /locus_tag="PK242_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOZ94476.1"
                     /translation="MKRMLINATQAEERRLAIVDGQKLLDYEIEIEGREQRKGNIYKA
                     VVTRVEPSLEACFVDYGEDRHGFLPFKEISKQYFAEGVSPSQARIQDVIKEGQELLVQ
                     VEKEERGNKGAALTTFI"
BASE COUNT          975 a         2320 c         2173 g         1017 t
ORIGIN      
        1 ctcagccggc ggtgtttgcc atcggcccct gaggcgctgc cgtcgccgcc ccgaaccgcc
       61 ctcccatcac acccagacgg cagcgccgcc atgtccaagc aacaagctca cccctcgcca
      121 tcgtccgccg ccaacggcgt gccggccctg tttgaccgcg ccgtgcgcct gttcaacggc
      181 ggccagccgg atcaggccgc ccccttgtgc cagcaggtgc tccagcagca gccaaaccac
      241 gcccatgcct ggcaactgct gggcatggtg cacgtggtcg gcaaccgccc ggccgaggcc
      301 ctgccggcgc tgcagcgggc gctggcgctg aacccgcggc agacccagat cctgtgcctg
      361 ctcggcacgg tgcttgcccg actggggcaa tccgagcagg ccatcgacca cttccgtcag
      421 gcgctcgcgc tggcacccca ggacgccaac atctggtacg acctgggcaa tgtgcagcac
      481 ggactgggtc agcacctcca ggcgctggcc agccacgatc gggcgctggc gctggccccg
      541 gggcacgccg acgcctggct caaccgcggc catgccctgc tggaactcca ccgcctgggc
      601 gacgccatcg ccagcttcga gcgcgccatc gacctgcgcc cccgggccag cacctggctg
      661 ttgttgggca gcgcgctgga gcaggcgcag cgcctgccgg acgcgctggc ctgcttcgag
      721 aaagtcgtgg ccctggagcc ggacaacctg gacggctggc tgaagttcgg cggcgtgctg
      781 ctgcagcttg ggcgccccga cctggcgctc gaacgcatcg aggtggcgca gcgcctggcg
      841 ccgcagtcgc cccgcgtgct gctgacccag gcggccgccc tggccaagac ccgcggcgac
      901 gcggcggcgc tgatgccgct gctgcaagcc catgcgctgg cacccgacga tctgtccgtc
      961 tccatgcagt tgctgtactt catgctcaag accgcccact gggaaggtct gaccgaggta
     1021 ttggctcaga cgctgcgcca ctggcgcgcc ggtgcccgcg acgccaacac gttgacgctg
     1081 ctggcccacc ccgacgtcag cgccgccgat ctgctgcaga ttgcgcaggc gtatcacgcc
     1141 ccactcaacg acgcctttcg cgccatgcac gcgaaggtgg cgcgtggcgc accggccggg
     1201 cgccggctgc gactgggtta cctgtcgtcc gatctgcggc accacgcggt cggctttctg
     1261 atgaccggcg tgttcgaagc ccatgaccgc cagcgcttcg aaaccttcgc gttctccatc
     1321 tacccgcaac ccgatcggtc gcccgagcgg cagcgcgcgc aggcggcctt cgatcatttc
     1381 atcgacatcc accacctggg cgatgacgag gccgccgccc tgatcgctgg ccacggcatc
     1441 gatctgctgg tcgatctcaa cggcctgacc accttcagtc ggcccggcat cctggcccgc
     1501 cggccagcgc cggtgcaggt gcagtacctc ggcttcccgg gcaccagcgg gctggcggcg
     1561 atcgactaca tccttggcga ccgctgggtc acgccggtcg agcaggcgca ggcgttttcc
     1621 gaacacatcg tgcgcatgcc cgactgcttc caggccaacg acgaccagcg ccgcttcgcg
     1681 gccgcaaccc cgacccgccg ggaactgggg ttgccggacc agggcttcgt gttctgctgc
     1741 ctgaacaaca gctacaagat cctgcccggc gtgttcgatg gctggatgcg gctgctgcaa
     1801 aacacacccg gttcggtgct gtggctgctg ggcgacaacg acaccgcccg cagcaacctg
     1861 cgcgcccacg cgcaggcgcg cggggtggcg cccgagcgcc tggtgttcgc cgaacggcgg
     1921 ccctacgacc agtacctggc ccggtatcgc caggccgatc tgtttctgga cacgctgccc
     1981 ttcaacggcg gcaccacggt cagcgatgcg ctgtgggccg gtctgccggt gctgacccag
     2041 gtcggcgacc gctttgccgg ccgcatggcc gccagcctgc tggactgcat cggcctgccc
     2101 gagctgatca cccacagcca gcccgagtac gaggcgctgg cgcatcggct ggccaccgag
     2161 ccggggctgc tgcagggcct gcgcgatcga ctggccgcca acatccggac ccgcccgttg
     2221 ttcgacacgc gccgcttcac ccgccacctg gagcgcgcct tcgagcacat gatcgagcgc
     2281 cacgcccagg ggctgccgcc acagggtttc gacgtggcgg agctgccgct ggcgcagtcg
     2341 gcctgaggat tcggccggaa cgcggcggcc gaccgccggc catctgcggg cctgcgccgc
     2401 gcccaacggc cgcctgtgtt gggctcagcg ccagcccggc gcgccgcgca gcgccgccag
     2461 cgccccctcg ccgacctgcg cccccagccg cttggcaaag cgctcggcga agttctgctt
     2521 gttggtgtag tccaccagct tctcggcctt gaccacctcg cgcgccacca tgtccaggct
     2581 gccgagttgg tcggccaggc cgagctgcac cgcctgctcg ccggtccaga acaggccgct
     2641 gaacatatcc ggggtttcct tcaggcgctc gccacggccc tggcgcacca cgtcgatgaa
     2701 ttgctggtgg atctggtcca gcatgcgctg ggcatgggcg cggtgggctt cggactgcgg
     2761 gctgaaggga tcgagaaagc ccttgttctc gcccgccgtc agcagccggc gctcgacgcc
     2821 cagcttttcc agcgccccga cggcaccgaa gccgtcgatc agcacaccga tgctgcccac
     2881 caggctggcc ttgtcgacgt agatgcggtc ggcggcggcg gcgatgtagt aggccgccga
     2941 ggcgcagctt tcttccacca cggcgtacac cggcttgtcg tgcagcgcgc gcaggcggcg
     3001 cacctcgtcg tggatcaggc ccgcctgcac cgggctgccg ccgggcgagt tgatcagcag
     3061 caccagcgcc tgcgcgccct tgtcttccat ggcgctgcgc agcgcgggca ggatgtactc
     3121 ggcgctgccc tcgccggtgt cgctgatctc gcccttgatc gacaccatgg cggtgtgggc
     3181 gctgctgggc gtggcgctgg gcaggtggtc ggaccacagc agccaggcca gggcgccgat
     3241 caggcccagc cacagcacgg tgcgcaaggt gcgccagcgg cgcgcggcgc ggcgctcgcg
     3301 cagctcggcg aacagcagtt tctcgatcgt ggcgcgctcc cagggcaccg gctggccgtt
     3361 cacatacact tcgttttcca tagcttgtgg cgcttgctgc gcaagggttt gaacttgatt
     3421 tgaggctcgg ccttgggctt cagaaggcga tcggccgcag cgccccggtg ggctgccagt
     3481 gcacctggcc ggccgcttcg atcagctcga tgcgaaccag ctgcgcgccg ctgcaggggc
     3541 cggccacgca atggccggtg tccggggcgt aggtggcgcc atgggtggcg cacagcagcc
     3601 attggccgct gtcgtcgaaa aagcggttgg gctgccagtc catttccatc ggcacatggg
     3661 cgcagcggtt gaggtaggca tgcacccggc cttcgaagcg cacggcaaag gcgcgcaggt
     3721 tttcgccacc atgcgcgacg tcgaacggca ccgccagccc gccttcctgc aggtcggcgc
     3781 tgtcgcacag gtaaaccggg ctcagatcgt tgtccatatt gcgccagcag ctatcaaatc
     3841 aatagttttc gaggtgacgg atcaggcatt gtcgctcagc caccggtgca actcggccac
     3901 cgagtgggcg acctggcgcg ggcgcagggc ctcgaagtcg tcgggtgcgt gggccccgta
     3961 gctcacgccg acgctggcgc agccggcgtt cagcgccatc tgcagatcgt gggtggtgtc
     4021 gccgatcatc agggtgcgct gcggctcgac accgaactcg cgcatcagct cgtgcagcat
     4081 gcgcgggtgc ggcttgccgg cggtttcgtc ggcggtgcgc gaaccatcga acacgccctg
     4141 cagctggcgc gaggccagca cctcgtccag gccgcgccgg ctcttgccgg tggccacgct
     4201 cagccagtgg tggcgcgcgc gcagctcggc cagcatgggc agcacgccct cgaacaggct
     4261 gatgtcgtgc tggcgcgcca ggtagtggtg acggtagcgc gccgccagct cggggtactt
     4321 gtcggccggt acgtcgggcg cggccctggc cagggccggc accagcgcca tgccgatcac
     4381 ataggcggcg gcggcgtcgc tcgggcgcgc gccacccacg tccagcaccg cctgctggat
     4441 gctgcgcacg atgatggcgg tggagtcgaa cagggtgccg tcccagtcga agcagatcag
     4501 gtcaaactgg cgtggacggc tcatggggcg ggcgcaaggt gcttggtgaa tgagagcaga
     4561 tcgggcggca gcgcggcctg cagttcgatg cgctcagcgc gctcggggtg gctgaactgc
     4621 aggcgccagg cgtgcaggaa catgcgtctc agcccctgtt tctgcaaggc cttgttgcgc
     4681 tcgaaatcgc cgtatttgtc gtcgccggca atcgggtggc cctgggccgc caggtgcacg
     4741 cggatctggt gcgtgcggcc ggtcttgatg gtgacttcga gcagggtgag cgccgccagc
     4801 cgctggcgca ccttcaccag ggtgatggcg cgcatgccgt cggggtcgtc cggcgccacc
     4861 acgcgcacgc gccgctcgcc gtcggccagc aggtatttgt gcaacggcgc gtcgatcacc
     4921 cggcgcgccg ccggccactc gcccagcacc agggcgaggt aggtcttgcc ggtctggcgc
     4981 tcacggaact ggtcctgcag gtgcttgagc gcgctgcgct tcttggccag cagcaggatg
     5041 ccgctggttt cgcggtccag ccggtgcacc agctccagaa agcgcagccc gcccggctgc
     5101 ccgtagccgc gcgcctggcg cagctgctcg atcacgccga agctcacgcc cgagccgcca
     5161 tgcacggcca cgccggccgg tttgtcgatg gcgatcaact gctcgtcttc cagcaggacg
     5221 gggaattcgc gcgccggcgc cgcccggccg tccaggtgtt ggacgggcgc cgccacccgc
     5281 accggcggca cgcgcacctc gtcgccctcg ctcacccggg tgtcggcggc ggcgcgcccc
     5341 ttgttcaggc gcacctcgcc ggagcggatg atgcggtaga catgggtctt gggcacgccc
     5401 ttgaggtggc gcagcaggta gttgtcgagc cgctggccgc cgctgtcggc gtccaccgtc
     5461 agccgcagca ctgcgggttt ggccgcgggc accgtggggg cggcctgggc acctataatg
     5521 cgattcactg gcaacccgcc gtaagtggtt gaatctgaac tggaagctgc atcccgaaaa
     5581 cgggaagtct attccaccgg cccatttccg cggcctgcca gtgaaaaggt cgatgccgtg
     5641 cctgcaccag aaagcgccct gccccacaag agtcggcagc cacgccagaa cagcgcagac
     5701 cggtcgacgc actgaaacac ccaacacgga atacccaaga tgcgcaggtc gccccctggg
     5761 ccgcctgcgg atcggatcga agcgagacac acgtggtgct gatggtctgg atcatggctt
     5821 tcgggcgctg gctgcggctg gcgctttttg agcctttttc gccgccaaat cgcaccccgc
     5881 aagcgccggc agctactttt tatgtagcaa ttttgcgccc ggcaccgctc gcccccatcc
     5941 acgcgccgcc gcctgaccgc ggccgctgaa cccccgttca gcggccacag ccggcgcacc
     6001 acgcgcctgc tgcatcccgc cctcagtcgc ccgccggccg gttccgtccg tttcagtgcg
     6061 ccgtcccagg catcgtgggt tcccacgagg aaccagggac tgttattgca tgaaggaacg
     6121 cgcatcatga aacggatgct gatcaacgcc acgcaggccg aagagcgccg cctggccatc
     6181 gtcgacggac agaaactgct cgactacgaa atcgagatcg aagggcgcga gcagcgcaag
     6241 ggcaacatct acaaggccgt cgtcacgcgc gtcgagccgt cgctggaagc ctgcttcgtc
     6301 gactacggcg aggaccgcca cggctttctg ccgttcaagg aaatctccaa gcagtacttt
     6361 gccgagggcg tctcgcccag ccaggcgcgc atccaggacg tcatcaagga aggccaggaa
     6421 ctgctggtgc aggtcgagaa ggaagagcgc ggcaacaagg gcgcggcgct gaccaccttc
     6481 atcag
//
LOCUS       DAOOOG010000148         6233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_14956_length_6233_cov_33.684202, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000148 DAOOOG010000000
VERSION     DAOOOG010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14956_length_6233_cov_33.684202"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /gene="ugpE"
                     /locus_tag="PK242_10425"
     CDS             complement(<1..661)
                     /gene="ugpE"
                     /locus_tag="PK242_10425"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HOZ94477.1"
                     /translation="MVERRPGLTLFSHLVLLLGIAVVAFPIYLAFVASTHTRDAIVQV
                     PMPLLPGGHLLDNYGAALFGAKTQGAHVVVGRMMWISFVTAMVIAVGKIAISLLSAFA
                     IVYFRFPFKKIVFWMIFITLMLPVEVRILPTYKVLSDLNMLNTYAGLTVPLIASATAT
                     FLFRQFFLSVPDELVEAARIDGAGPMRFFKDILVPLSQTSIAALFVIQFIYGWNQYLW
                     PL"
     gene            complement(698..1579)
                     /gene="ugpA"
                     /locus_tag="PK242_10430"
     CDS             complement(698..1579)
                     /gene="ugpA"
                     /locus_tag="PK242_10430"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HOZ94478.1"
                     /translation="MEKRALFRSGWLPWLLIAPQMAVILVFFFWPASQALMQSVQAED
                     AFGTSTTFVGLDNFVTLFEDPAYVESFKVTAIFSVLVAGCGIALSLVLAIFADRVVRG
                     ALVYKSFLIIPYAVAPAIAGVLWVFMFSPSIGVVSYGLRQMGIDWNHLLNSGQALTLI
                     VVAAVWKQISYNFLFFLAGLQSIPKALIEAAAIDGAGRLRRFFTIQFPLLSPTTFFLL
                     VINVVYAFFDTFAIVHATTEGGPGRDTAILVYKVWHDGFKALDLGGSAAQSVVLMAIV
                     VGLTVIQFRYVEKKVQY"
     gene            complement(1688..2995)
                     /gene="ugpB"
                     /locus_tag="PK242_10435"
     CDS             complement(1688..2995)
                     /gene="ugpB"
                     /locus_tag="PK242_10435"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HOZ94479.1"
                     /translation="MKFRLAAASLAAALAASPALAQTEIQWWHSMSGPLGEWVGDLAK
                     QFNASQSAYRVVPTYKGQYDESMTAAIAAFRAGNAPDILQVFEVGTATMMASKGAIKP
                     VGEVMTAGGAKFDPSVYISAVAGYYTAPNGQMLSFPFNSSTTVFYYNKDAFKAAGLDP
                     EKAPTTWPEVFAAADKLKAGGHKCPFTTSWISWTQLESFSAWHNTLFASHNNGFGGTA
                     ARLAFNSPLHVRHFENLAKMAKNGTFVYKGRNNAADAAFPSGECAMMTGSSGLYARVA
                     KDAKFKYGISTLPYYPDVKGAPQNTVIGGASLWVMAGKSPEKYKGVARFFDFLSDTKV
                     QSESHRRTGYLPITTGAFKLTDASGFYKDKPGTDVAVTQMIRKTTDKSRGIRLGNFVQ
                     IRTIVDEETEQIWAGKRSAKEALDAAVKRGNEQLARFQKANRN"
     gene            3245..>6233
                     /locus_tag="PK242_10440"
     CDS             3245..>6233
                     /locus_tag="PK242_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HOZ94480.1"
                     /translation="MNAPTQPAHLMAAADDEAAPRLREIPYNYTSFSDREIVLRLLGE
                     RAWEIVQTLRSERHTGRSSRMLYEVLGDIWVVRRNPYLQDDLLDNPRRRALLVEALRH
                     RLAEVEKRRHPEADAARDALVGELLTAAHRVVDAFDRSFDKVAELRRRSARVLGRHTH
                     KDNIKFDGLSRVSHVTDATDWRVEFPLVVLTPDSEAEMAALVKGCIELGLTIIPRGGG
                     TGYTGGAIPLDWQSAVINTEKLIDIGRVEPRRLPGLDRDVPTIWTEAGVVTQRVADAA
                     EDAGLVFAVDPTSAEASCIGGNIAMNAGGKKAVLWGTALDNLASWRMVTPAGEWLEVS
                     RIGHNLGKIHDAELARFELVYFDASGKRELRREQLAIPGASFRKAGLGKDVTDKFLSG
                     LPGVQKEGCDGLITSARWVLHRMPEHTRTVCLEFFGHAKDAVPSIVEIKDFMFAEQKR
                     SGTLLAGLEHLDDRYLRAVGYSTKSKRGGLPKMVLIGDIAGDDPDAVARAASEVVRIA
                     NSRAGEGFTAVSPEARKKFWADRKKTAAISRHTNAFKINEDVVIPLPRMAEYTDGIER
                     INIELSLSNKIELADELEAFLGRGQLPLGAGAPAPELLQEKVDLALAQVREVRALWQG
                     WLADIDRLFPQLQDHSLRASWKTQIREPLRAIFTGSDFEPLMAALGEIQQRVLKGRVW
                     IALHMHAGDGNVHTNIPVHSDNYAMLQTAHAAVDRVMALARRLDGVISGEHGIGITKL
                     QYLSDDELAPFADYKRRVDPEGRFNRGKLLRNRELPAQPGRRLRADLSNAFTPSFGLM
                     GHESLLLQQSDLGAIADSIKDCLRCGKCKPECSTHVPRANLLYSPRNKILATSLLIEA
                     FLYEEQTRRGVSIQHWAEFEDVADHCTVCHRCLAPCPVKIDFGDVTMNMRNLLRKMGK
                     KSVRPGNAAAMFFLNATDPRVINATRAVMTGVAFKAQRAAVDVLRVAGRRQTEHPPAT
                     VGPAPIREQVIHFVNKKLPGGLPT"
BASE COUNT         1093 a         2061 c         2147 g          932 t
ORIGIN      
        1 gcagcggcca caggtactgg ttccagccgt agatgaactg gatcacgaac agcgccgcga
       61 tcgaggtctg cgacagcggc accagaatgt ccttgaagaa gcgcatcggc ccggcgccgt
      121 cgatgcgcgc ggcctccacc agctcgtcgg gcaccgacag gaagaactgc cggaacagga
      181 aggtggcggt ggccgaggcg atcagcggca ccgtcagacc ggcataggtg ttgagcatgt
      241 tcaggtccga cagcaccttg taggtgggca ggatgcgcac ctccaccggc agcatcaggg
      301 tgatgaagat catccagaac acgatcttct tgaacggaaa gcggaagtag acgatggcga
      361 acgccgacag cagcgagatg gcgatcttgc ccaccgcgat caccatggcg gtgacgaagc
      421 tgatccacat catgcgcccc accaccacgt gcgcgccctg cgtcttggcg ccgaacagcg
      481 cggcgccgta gttgtcgagc aggtggccgc cgggcagcag cggcatcggc acctgcacga
      541 tggcgtcgcg cgtgtgtgtg gaggccacga aggccaggta gatcgggaac gccaccaccg
      601 caatgcccag cagcagcacc aggtgcgaaa acagcgtcag gccggggcgg cgttcaacca
      661 tggcggggcc tcgcggtggg gtgggcggtg tcgggcatca gtactgcacc ttcttttcga
      721 cgtagcggaa ctggatcacc gtcaggccga ccacgatggc catcagcacc accgactgcg
      781 cggccgagcc gcccaggtcg agcgccttga agccgtcgtg ccagaccttg tagaccagga
      841 tggcggtatc gcgtcccggc ccgccctcgg tggtggcgtg gacgatggcg aaggtgtcga
      901 agaaggcgta caccacgttg atcaccagca ggaagaaggt ggtgggcgac agcagcggaa
      961 actggatggt gaagaagcgc cgcagccgcc cggcgccgtc gatggcggcg gcctcgatca
     1021 gggccttggg gatggactgc aggccggcca gaaagaacag gaagttgtag gaaatctgct
     1081 tccacaccgc ggccaccacg atcagcgtca gcgcctggcc ggagttcagc aggtggttcc
     1141 agtcgatgcc catctgccgc aggccgtagc tgaccacgcc gatgctgggc gagaacatga
     1201 acacccacag cacgccggca atggccggcg ccacggcgta gggaatgatc agaaagctct
     1261 tgtagaccag cgcgccgcgc accacgcggt cggcaaagat ggccagcacc agcgacagcg
     1321 cgatgccgca gccggccacc agcaccgaga agatcgccgt caccttgaag gattcgacgt
     1381 aggccgggtc ttcgaacagg gtgacgaagt tgtccaggcc gacgaaggtg gtgctggtgc
     1441 cgaaggcgtc ttcggcctgc accgactgca tcagggcctg gctggcgggc cagaagaaga
     1501 acaccaggat caccgccatc tgcggcgcga tcagcagcca gggcagccag ccggagcgaa
     1561 agagggcgcg tttttccatg ggtggcgcga atgagaaaag cccgccggcg ggtgggtcgg
     1621 cgggcgatca gtctagcggg ttgcgccgcc ggccggcggc aggcgccgtc ggccggtgcc
     1681 cggcccttca gttgcggttg gctttctgga agcgtgccag ctgctcgttg ccgcgcttga
     1741 cggcggcgtc cagcgcttcc ttggcgctgc gtttgccggc ccagatctgc tcggtttcct
     1801 cgtcgacgat ggtgcggatc tgcacgaagt tgcccaggcg gatgccgcgc gacttgtccg
     1861 tggtcttgcg gatcatctgc gtcaccgcga cgtcggtgcc gggcttgtcc ttgtagaagc
     1921 cggaagcgtc ggtcagcttg aaggcgccgg tggtgatcgg caggtagccg gtgcgccggt
     1981 ggctttcgga ctgcaccttg gtgtcggaca ggaagtcgaa gaagcgcgcc acccccttgt
     2041 acttctcggg ggacttgccg gccatcaccc acaggctggc gccgccgatc acggtgttct
     2101 gcggcgcgcc cttgacgtcg gggtagtagg gcagggtgga gatgccgtac ttgaacttgg
     2161 cgtccttggc cacgcgcgcg tacaggcccg aggagccggt catcatggcg cattcacccg
     2221 aggggaaggc ggcatcggcg gcgttgttgc ggcccttgta gacgaaggtg ccgttcttgg
     2281 ccatcttggc caggttctcg aaatgccgca catgcagcgg cgagttgaag gccagccgtg
     2341 ccgccgtgcc cccgaagccg ttgttgtggc tggcgaacag ggtgttgtgc caggccgaga
     2401 agctctccag ctgcgtccag ctgatccagc tggtggtgaa cgggcacttg tggccgccgg
     2461 ccttgagctt gtcggcggcg gcaaacactt ccggccaggt ggtgggggcc ttttcggggt
     2521 ccagcccggc ggccttgaag gcgtccttgt tgtaatagaa cactgtcgtc gagctgttga
     2581 acgggaagct cagcatctgc ccgttgggcg cggtgtagta gccggccacg gccgagatgt
     2641 agacgctggg gtcgaacttg gcgccgccgg cggtcatgac ctcgcccacc ggcttgatgg
     2701 cgcccttgga ggccatcatg gtggcggtgc cgacctcgaa cacctgcagg atgtcgggcg
     2761 cgttgccggc gcggaaggcg gcgatggcgg cggtcatgga ttcgtcgtac tggcccttgt
     2821 aggtgggcac cacccggtag gcgctctggc tggcgttgaa ctgcttggcc aggtcaccca
     2881 cccattcgcc cagcgggccg ctcatcgagt gccaccactg gatttcggtt tgcgccagcg
     2941 cgggcgatgc ggcgagggcg gccgccaggc tggcggcggc aagacggaat ttcatgaaac
     3001 ctccttggac aatggggaaa atgcggcgcc acagcccctg cgacgcacag caccatgatg
     3061 ggacaggcgg attgcacgcc catgtcagcc gcgtgacaag cgggtttgta cgcaagccgg
     3121 ggcccgacct cagaccctgt gtgtgaatgt tgcaccgcag taaaatcgcc ggccagccgg
     3181 acgccgggtg cgccttgtgc gcttgttttg ccgtggcttg ttggcgccgc gctgggccca
     3241 ccacatgaac gctccgaccc agcccgccca cctgatggcc gccgccgatg acgaggcggc
     3301 gccgcgcctg cgcgagatcc cctacaacta caccagcttt tccgaccgcg agatcgtgct
     3361 gcggctgctg ggcgagcgcg cctgggagat cgtgcagacg ctgcgcagcg aacgccacac
     3421 cggccgctcc tcgcgcatgc tgtacgaggt gctgggcgac atctgggtgg tgcggcgcaa
     3481 cccctacctg caggacgacc tgctggacaa cccgcgccgt cgcgcactgc tggtcgaagc
     3541 gctgcgccac cgcctggcgg aggtcgaaaa gcgccgccac cccgaggcgg acgccgcgcg
     3601 cgacgccctg gtcggcgagc tgctgacggc ggcccaccgg gtggtcgatg ccttcgatcg
     3661 ctcgttcgac aaggtggccg agctgcgccg ccgcagcgcg cgcgtgctgg gtcgccacac
     3721 gcacaaggac aacatcaagt tcgacggcct gtcacgcgtc agccacgtga ccgacgccac
     3781 cgactggcgg gtggagtttc cgctggtggt gctgacgccc gacagcgagg ccgagatggc
     3841 ggcgctggtc aagggttgca tcgaactggg cctgaccatc atcccgcgcg gtggcggcac
     3901 cggctacacc ggcggcgcca ttccgctgga ctggcagagc gcggtcatca acaccgaaaa
     3961 gctgatcgac atcggccgcg tcgagcccag gcgcctgccc gggctcgacc gcgacgtgcc
     4021 caccatctgg accgaagccg gcgtggtgac ccagcgcgtg gccgacgcgg ccgaagacgc
     4081 cggcctggtg ttcgcggtcg acccgactag cgccgaagcc agctgcatcg gcggcaacat
     4141 cgccatgaac gccggtggca agaaagccgt gctgtggggc acggcgctgg acaacctggc
     4201 gagctggcgc atggtcacgc ccgccggcga atggctggaa gtgagccgca tcggccacaa
     4261 cctgggcaag atccacgacg ccgagctggc gcgcttcgag ctggtctatt tcgacgccag
     4321 cggcaagcgt gaactgcgcc gcgagcagct ggccatccct ggcgccagct tccgcaaggc
     4381 cggcctgggc aaggacgtga ccgacaaatt cctcagcggc ctgcccgggg tgcagaagga
     4441 gggctgcgac ggcctgatca ccagcgcgcg ctgggtgctg caccgcatgc ccgagcacac
     4501 gcgcaccgtg tgcctcgaat ttttcggcca cgccaaggac gccgtgccca gcatcgtcga
     4561 gatcaaggac ttcatgttcg ccgagcagaa gcgcagcggc accctgctgg ccgggctgga
     4621 gcacctggac gaccgctacc tgcgcgccgt cggctacagc accaagtcca agcgcggcgg
     4681 tctgcccaag atggtgctga ttggcgacat cgccggcgac gaccccgacg ccgtggcgcg
     4741 cgccgccagc gaggtggtgc gcatcgccaa ctcgcgcgcc ggcgagggct tcaccgccgt
     4801 gagccccgag gcgcgcaaga agttctgggc cgaccgcaag aagacggccg ccatcagccg
     4861 ccacaccaac gccttcaaga tcaacgagga cgtggtgatt ccgctgccgc gcatggccga
     4921 atacaccgac ggcatcgagc gcatcaacat cgagctcagc ctgagcaaca agatcgagct
     4981 ggccgacgag ctggaggcct ttctcggtcg cggccagctg ccgctgggtg ccggcgcgcc
     5041 ggcgcccgag ctgctgcagg aaaaggtcga cctcgccctg gcccaggtgc gcgaggtgcg
     5101 cgcgctgtgg cagggctggc tggccgacat cgaccggctg ttcccccagc tgcaggacca
     5161 cagcctgcgc gccagctgga agacccagat tcgcgagccg ctgcgcgcca tcttcaccgg
     5221 cagcgacttc gagccgctga tggcggcgct gggcgagatc cagcagcgcg tgctgaaggg
     5281 gcgcgtgtgg atcgccctgc acatgcacgc cggcgacggc aacgtgcaca ccaacatccc
     5341 cgtgcacagc gacaactacg ccatgctgca aaccgcgcac gccgcggtcg accgcgtgat
     5401 ggcgctggcg cgccgcctgg acggcgtgat cagcggcgag cacggcatcg gcatcaccaa
     5461 gctgcagtac ctgagcgacg acgagctggc gccgtttgcc gactacaagc ggcgcgtcga
     5521 tcccgagggc cgtttcaacc gcggcaaatt gctacgaaat agggagctgc cggcgcagcc
     5581 tgggcgccgt ctgagggccg atttgagcaa tgccttcaca cccagcttcg ggctcatggg
     5641 gcacgaatcg ttgctgctgc agcaaagcga cctgggcgcc atcgccgact cgatcaagga
     5701 ctgcctgcgc tgcggcaaat gcaagcccga gtgcagcacc cacgtgccgc gcgccaacct
     5761 gctgtacagc ccgcgcaaca agatcctggc cacctcgctg ctgatcgagg ccttcctgta
     5821 cgaggagcag acgcgccgcg gcgtcagcat ccagcactgg gccgagttcg aggacgtggc
     5881 cgaccactgc accgtgtgcc accgctgcct ggcgccctgc ccggtgaaga tcgacttcgg
     5941 cgacgtcacc atgaacatgc gcaacctgct gcgcaagatg ggcaagaaga gcgtgcggcc
     6001 cggcaacgcc gccgccatgt tcttcctgaa cgccaccgat ccgcgcgtca tcaacgccac
     6061 ccgtgcggtg atgaccggcg tggccttcaa ggcgcagcgc gccgccgtcg acgtgctgcg
     6121 cgtggccggg cgccggcaga ccgagcatcc gcccgccacc gtcggcccgg cgccgatccg
     6181 cgagcaggtg atccacttcg tcaacaagaa gctgcccggc ggcctgccca cca
//
LOCUS       DAOOOG010000149         6230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_14961_length_6230_cov_27.969393, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000149 DAOOOG010000000
VERSION     DAOOOG010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14961_length_6230_cov_27.969393"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..914
                     /locus_tag="PK242_10445"
     CDS             273..914
                     /locus_tag="PK242_10445"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HOZ94481.1"
                     /translation="MTQRLASRLAVGLPTLLLWALLAGSALWWWLRAGQPAAPQPAPV
                     AGGAPAARTVDSAQVARVLGATAAAPAAPAAAPELGGRVVLKGVVTHGARGAALIALD
                     GQPARPLRVGAELEGVEGGWRLRSVAPHGALLAAAGGRELRLEMPPLERRSSGSAAAV
                     PSHPAPPVPAGRPGTSRAGPMAPVVPTPPTLPAGMAVSPQPQAFPAPGAAPAQ"
     gene            complement(1086..2657)
                     /gene="ilvA"
                     /locus_tag="PK242_10450"
     CDS             complement(1086..2657)
                     /gene="ilvA"
                     /locus_tag="PK242_10450"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HOZ94482.1"
                     /translation="MTPPASAPTGAAALSPTDYLKQVLTARVYDVAVESDLDPARSLS
                     RRLHNKVTLKREDQQPVFSFKLRGAYNKMAHLTPEQLHKGVICASAGNHAQGVALGAH
                     KLGTRAVIVMPTTTPQVKVDAVRALGGEVVLAGDSYSDAYEHALGLQAEQGLTFIHPF
                     DDPLVIAGQGTVGLEILRQHKGPLHAVFVPIGGGGLISGVGACIKAVRPEVKVIGVQM
                     SDSDAMAQSVRQGRHVQLADVGLFADGTAVKRVGDETLRLAGAVVDEFITVDTDAVCA
                     AIKDVFIDTRSIVEPSGAMAVAALKQYVATHKTRGESYVAVLSGANMNFDRLRFVAER
                     AEVGEEREALFAVTIPEARGSFRRFLEVIGSLPGGARNVTEFNYRISDAAQAHVFIGL
                     TTAARGESPKIAAHFERHGLKTVDLTHDELAKEHVRHMVGGRSSLAQHERLLRFVFPE
                     RPGALLKFLSLMQPSWNISLFHYRNQGADYGRILVGMQVPASDDAAFRRFLAELGYPW
                     VEETDNPAYRLFLQR"
     gene            2993..3442
                     /locus_tag="PK242_10455"
     CDS             2993..3442
                     /locus_tag="PK242_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOZ94483.1"
                     /translation="MECTVSWTGAAGTRSGMGFVAETGSGHVIAMDGAPDAARPEFGG
                     QNLAPRPMETLLAGTGGCTAYDVVLILRRGRHDVRGCSVRLSSERASEDPKVFTAIHM
                     HFTVSGRGVPAAAVERAIQLSHDKYCSASIMLGKTATITTSFEVVEV"
     gene            complement(3688..4323)
                     /gene="coq7"
                     /locus_tag="PK242_10460"
     CDS             complement(3688..4323)
                     /gene="coq7"
                     /locus_tag="PK242_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HOZ94484.1"
                     /translation="MPTAIDRLLAAADSGLRTLLAPPRAARPSPAAAVTASTPLTEDE
                     QRLSGALMRVNHVGEICAQALYTAQALGTSDPALRRQLDAAAREETDHLAWTRERLDQ
                     LGDRVSLLNPLWFAGAFGIGLLAGRIGGDRLSLGFVVETERQVEAHLASHLDRLPAAD
                     SASRAIVAQMKDDEARHADAALHAGGVALPAPVRGLMRLAAKVMTVTAHRI"
     gene            4850..5779
                     /locus_tag="PK242_10465"
     CDS             4850..5779
                     /locus_tag="PK242_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOZ94485.1"
                     /translation="MKKSLIALAVVALSGAAMAQSSVTLYGVADAGLGKIKSAGNDAS
                     NKAQFTSASTMNNGDSRLGVRGVEDLGAGLKIGFNFETGLDLDDGATTTSSFWARQAN
                     LSVGGNWGTVKLGRQFTPSYLTTATYEVTGTANYSALGKTYRYAGIGSRANSAFAYIT
                     PNFGGFTAAAAYVSKHDVGANVWDLGLMYNNGPIAAGLSANKVSGGKTNYQLGGKYSF
                     GNFALAASYNQASDAGGLRRRGASIGGSATFGATSIALDLTRDTKNEWTGKKYTNGVL
                     EAKYALSKRTFVYGAFLRYDGDNNYGVGVRHNF"
BASE COUNT          943 a         2130 c         2210 g          947 t
ORIGIN      
        1 accaccatca gctcgatcag ggtgaagccg gcggcgcggc gcgcgcgggc ggcgatgggg
       61 ggcgagaggg tcatggcggg ggtctgcagg cgagcttcaa tacagcacgg ccgcgggggc
      121 gcccgccgct gtcgtggtcg gtgtgccggg caggggcgcg agccggcctg cctgcacatc
      181 catgcggcgc agccgcagcg ggcgagcgct ccacggccgt ggtgggttca atgataatcc
      241 ccgacgatga ccgcgatcgc tgccgccacg ccatgaccca acggctcgct tcccgcctgg
      301 ccgtcggcct gccgaccctg ctgctgtggg cgctgctggc cggcagtgcg ctgtggtggt
      361 ggctgcgcgc cgggcagccg gcggcgccgc agccggcgcc ggtggcgggc ggcgcgccgg
      421 ccgcgcgcac ggtggacagc gcccaggtgg cgcgggtgct gggcgccacg gcggcggcgc
      481 cggcagcgcc cgccgccgcg cccgaactgg gcggtcgggt ggtgctcaag ggcgtggtga
      541 cgcacggcgc gcgcggcgcg gcgctgatcg cgctggacgg ccagccggcc cggccgctgc
      601 gcgtgggcgc cgagctggaa ggggtggaag gcggctggcg cctgcgcagc gtggcgccgc
      661 atggcgcgct gctggcggca gccggtggcc gcgagctgcg gctggagatg ccgccgctgg
      721 agcggcgcag cagcggcagc gcggcggcgg tgccttcgca tccggcgccg ccggtgccgg
      781 ccgggcgccc gggaacgtcg cgggccggtc cgatggcacc cgtggtgccg accccgccga
      841 cgctgccggc cggcatggcg gtgtcgccgc agccgcaggc ctttccggcg cctggcgccg
      901 cgccggcgca gtaggcggaa tctggggagc gggcgccggc ccttggctga aaggcgccga
      961 cagccatcaa cgacagcgca cccggggcag ggccgaaccc cgccttcgcc ccggcggcgc
     1021 cctcgtcatc gaagctcggc gcgcgctttt cccattcgcc tgcgccgcct tgggtcaggc
     1081 gcgactcaac gctgcaggaa cagccggtag gccgggttgt cggtttcctc gacccagggg
     1141 tagcccagct cggccagaaa gcgccggaac gcggcgtcgt cgctggccgg cacctgcatg
     1201 ccgaccagga tgcggccgta gtcggcgccc tggttgcggt agtggaacag gctgatgttc
     1261 cagctcggct gcatcagcga caggaacttc agcagcgccc cggggcgctc ggggaacacg
     1321 aagcgcagca gccgctcgtg ctgcgccagc gacgagcgcc cgccgaccat gtggcgcaca
     1381 tgctccttgg ccagctcgtc gtgcgtcagg tccaccgtct tcaggccgtg gcgctcgaag
     1441 tgggcggcga tcttggggct ttcgccgcgc gcggcggtgg tcaggccgat gaacacgtgc
     1501 gcctgcgcgg cgtcgctgat gcggtagttg aactcggtca cgttgcgcgc gccacccggc
     1561 aggctgccga tcacctccag aaagcgccgg aagctgccgc gcgcctcggg gatggtgacg
     1621 gcgaacagcg cctcgcgctc ctcgcccacc tcggcgcgct cggcgacgaa gcgcaggcgg
     1681 tcgaagttca tgttggcgcc cgacagcacg gccacgtagc tttcgccgcg tgtcttgtgc
     1741 gtggccacgt actgcttgag ggccgccacc gccatggcgc cggacggctc gacgatgctg
     1801 cgcgtgtcga tgaacacgtc cttgatggcg gcgcacacgg cgtcggtgtc gacggtgatg
     1861 aactcgtcca ccaccgcgcc ggccaggcgc agggtttcgt cgcccacacg cttgacggcg
     1921 gtgccgtcgg cgaacaggcc gacgtcggcc agctgcacat ggcggccctg gcgcaccgac
     1981 tgcgccatgg cgtcggagtc gctcatctgc acgccgatca ccttgacctc aggccgcacc
     2041 gccttgatgc aggcgcccac gccgctgatc agcccgccgc cgccgatggg cacgaacacc
     2101 gcgtgcagcg gccccttgtg ctggcgcagg atctccaggc ccaccgtgcc ctggccggcg
     2161 atcaccagcg ggtcgtcgaa cgggtggatg aaggtcaggc cctgctcggc ctgcaggccc
     2221 agggcgtgct cgtaggcgtc ggagtagctg tcgccggcca gcaccacctc gccgcccagc
     2281 gcccgcacgg cgtccacctt gacctgcggg gtggtggtgg gcatcacgat caccgcccgc
     2341 gtgcccagct tgtgcgctcc cagcgccacg ccctgggcgt ggttgccggc gctggcgcag
     2401 atcacgccct tgtgcagctg ctcgggcgtc aggtgcgcca tcttgttgta ggcgccgcgc
     2461 agcttgaagc tgaacaccgg ctgctggtcc tcgcgcttga gcgtcacctt gttgtgcagg
     2521 cggcggctga ggctgcgcgc cgggtcgagg tcggactcca ccgccacgtc gtagacgcgg
     2581 gcggtcagca cctgcttgag gtagtcggtc gggctcaggg cggcggcgcc ggtgggcgcg
     2641 gaggcgggcg gtgtcatgaa gcgggtgggc gcgcgaatga agccgaaatc atacggtccg
     2701 gggcaccggc ggcgccctgg cacggtgcgg tgcagcaaaa aaaagccccg gagccgcagc
     2761 gccggggctt gaatccacct tgttgaaggg gtggaggaga caagctgtgc gaatgaaacc
     2821 tcactcgcat gaacgcaagt ataggcgaaa agcacgaccg ttcgccaagt gtttgctgca
     2881 atgcaacagc gggactgtgg cgaaagcggt acttttgctc ggctggcgcg gggtgcgcgc
     2941 cgcatccgct acgcttgcgg cctgcaatca ggacacggaa aggatggcgc acatggaatg
     3001 cacggtgagc tggacgggcg cggcgggcac ccgctcgggc atgggatttg tggcggaaac
     3061 cggctccggt cacgtgatcg ccatggacgg cgcgcccgat gccgcgcggc ccgaattcgg
     3121 cggccagaac ctggcgccgc gcccgatgga gacgctgctg gccggcaccg gcggctgcac
     3181 cgcctacgac gtggtgctga tcctgcggcg cggccgccac gacgtgcgcg gctgctcggt
     3241 gcgcctgagc agcgagcgcg ccagcgaaga ccccaaggtg ttcaccgcca tccacatgca
     3301 cttcaccgtc agcggccgcg gcgtgccggc ggcggcggtg gagcgcgcca tccagctcag
     3361 ccacgacaag tactgctcgg cctcgatcat gctgggcaag acggcgacca tcaccacttc
     3421 attcgaggtg gtggaggtct gagaggttga gcgtcttttg tccatgcccg cctggcacgc
     3481 caaaacaccg ctgctcgtcg ttgcaaatgc tcgccatggc tcgggccaag gctgtgcttg
     3541 tgcagccagg atcggcggtg ttctgacgcg cctttcgggc aagaacgaaa aactctcagc
     3601 ctctgacgcc cgccgattgc tatcgaaatg aaagctgctg gcgctggcca gtagggcgtt
     3661 acgccacgat ttggtgctgc aaaccgctca gatgcggtgc gcggtgaccg tcatcacctt
     3721 ggccgccagc cgcatcagcc cgcgcaccgg cgccggcagc gccacgccgc cggcgtgcag
     3781 cgcggcgtcg gcgtggcgcg cctcgtcgtc cttcatctgc gccacgatgg cgcgcgaggc
     3841 gctgtcggcc gccggcagac ggtccaggtg gctggccagg tgggcctcga cctggcgctc
     3901 ggtctccacc acgaagccca ggctcagccg gtcgccgccg atgcgcccgg ccagcaggcc
     3961 gatgccgaag gcgccggcaa accacagcgg gttgagcagc gaaacgcggt cgcccagctg
     4021 gtcgagccgc tcgcgcgtcc aggccaggtg gtcggtttcc tcgcgtgcgg cggcgtcgag
     4081 ctgccggcgc agcgccgggt cgctggtgcc cagcgcctgc gcggtgtaga gcgcctgggc
     4141 gcagatttcg cccacgtggt tgacccgcat cagcgccccc gacaggcgct gctcgtcctc
     4201 ggtcaggggt gtcgatgcgg tcacggcggc ggccgggctg ggacgggcgg cgcgcggcgg
     4261 cgccagcagc gtgcgcaggc cgctgtcggc ggcggcgagc aggcgatcga tggcagtcgg
     4321 catggcgcga tgttagggca aacccagtgc agtgtttcgc gctgtcaggc acttacagaa
     4381 gccagaacaa ggcgtgcagc aaggcgcggc tggccaccca tgctggcggc cttggcaagc
     4441 ggcgcaacgc cgccgcaagc cttttttttc ccgcagggcg agccgccaag gggccatctg
     4501 gtttgtggcg tggccttgac atggctccac catgcctgcg gccacgcgcc gcgcagttgg
     4561 ccccttggcg actcgctaca agtacctggc agcgcgaaac actgcaccag atggtgcatg
     4621 tcataatgac ccgctagtca gtaaacccct agcagtggct gaaaagcaac aaagcgaggg
     4681 taaatactag tttacaaacc gcccatggca tagaattgcc tcacgttcgg gtggtgatcg
     4741 acatccctcc tgatcgggtg acgaccggcg ttcaagggcc agccatgaat tgtcattcgt
     4801 tgcttcctga gcgtccgttt cgcgtctcgc attacctgga gaaactgcaa tgaaaaaatc
     4861 cctcatcgcc ctggccgttg tggccctgtc cggcgccgcc atggcccagt cgtccgtcac
     4921 gctgtacggc gtggccgacg ccggcctcgg caagatcaag agcgccggca acgacgcgtc
     4981 caacaaggcc cagttcacca gcgccagcac catgaacaac ggcgacagcc gtctgggcgt
     5041 gcgcggtgtg gaagacctgg gtgccggcct gaagatcggc ttcaacttcg aaaccggcct
     5101 ggacctggac gacggcgcca ccaccacctc gtccttctgg gcgcgccagg ccaacctgtc
     5161 ggtcggcggc aactggggta cggtcaagct gggtcgccag ttcacgccga gctacctgac
     5221 caccgccacc tatgaggtca ccggcaccgc caactactcg gccctgggca agacctaccg
     5281 ctacgccggc atcggctcgc gcgccaacag cgcgttcgcc tacatcacgc ccaacttcgg
     5341 tggcttcacc gccgcggcgg cctatgtctc caagcacgac gtcggcgcca acgtgtggga
     5401 tctgggcctg atgtacaaca acggcccgat cgcggccggc ctgtcggcca acaaggtcag
     5461 cggtggcaag accaactacc agctgggtgg caagtacagc ttcggcaact tcgctctggc
     5521 cgcttcgtac aaccaggcgt ccgacgccgg tggcctgcgc cgccgtggcg ccagcatcgg
     5581 cggttcggcc accttcggtg ccacctcgat cgccctggac ctgacgcgcg acaccaagaa
     5641 cgagtggacc ggcaagaagt acaccaacgg tgtgctcgag gccaagtacg cactgtccaa
     5701 gcgcaccttc gtctacggcg ccttcctgcg ctacgacggc gacaacaact acggcgtcgg
     5761 cgtccgccac aacttctgat gaccgcatgg ccggcgcccc gacaaggggc gtgcggccga
     5821 ggctgcgacg gccgacgacc ggggtttccc gggccggctg tcgcacgcca gagcctctgc
     5881 ttgcgcaggc ggaggctgtc ccggcgcccg gcagggcgtt ttcagggacc aagaccgccc
     5941 cgaaggccgt cggccgggca gggttgtctt ttatttgcaa cgctcccaga ggttctgttg
     6001 tcggtctgca acagaacggg gctgtgctgt ccgatcgcgg gctgaattct ttgcaagttg
     6061 ctgccgggtc tggtgcaata gcgtcaactt cccggtcggg gaggttggcc cggggaaccg
     6121 gcgggaatcg cacacggacc cttcgcaccg gcgccctaag tgtttaacct tggagaactt
     6181 gcaatgaaaa aatctctgat tgccctggcg gtcctgggct ttgccggcgc
//
LOCUS       DAOOOG010000150         6146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_15243_length_6146_cov_23.026761, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000150 DAOOOG010000000
VERSION     DAOOOG010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15243_length_6146_cov_23.026761"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PK242_10470"
     CDS             complement(<1..283)
                     /locus_tag="PK242_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOZ94486.1"
                     /translation="MHTVAEILKSRQGTTLATLPPTATVREALQIMADRNIGSVLIVQ
                     GDALLGIFTERDYARRLVLKGLASADTLLADVMTTRLYVVNPHQTVQECL"
     gene            527..1207
                     /locus_tag="PK242_10475"
     CDS             527..1207
                     /locus_tag="PK242_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOZ94487.1"
                     /translation="MTAALTVISSMATRKLLGELIDKLAGAPVTVESVGGVDAARRVA
                     AGEVFDAVVLGADAIDRLIADGQVRAGSRVDLVRSGVALAVKAGAPRPDIGSAQAVRA
                     AVLAARTVGCSTGPSGVALARLFDHWGIAEQVQARLVVPPPGTPVGALIARGEVELGF
                     QQLSELMFLPGIDVLGPLPEPIQITTVFAGGVPTASRQPQAAQALLERLAAPDLTAIK
                     RANGMDSA"
     gene            1251..2237
                     /locus_tag="PK242_10480"
     CDS             1251..2237
                     /locus_tag="PK242_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOZ94488.1"
                     /translation="MVLRGARQALQVERRPLPQPGAGQVRLRVRACAVCRTDLHLQDG
                     DLPMARWPVVPGHEVVGTVDALGAATTGLRLGQRVGVPWLGGACGQCRYCLAGQENLC
                     DAPQFTGCTRDGGFATHIVADASFCVPLDHLALDDASAAPLLCAGLIGWRSLRLAGDD
                     ARVLGLYGFGAAAHLIAQVALAQGRRVHAFTRPGDTAAQQHARALGCTWAGDADQPAP
                     EPLDAAILFAPAGELVPLALRAVRKGGRVVCGGIHMSDIPSFPYALLWQERQLLSVAN
                     LTRADAREFFAALQALPPLRIHTTPYPLQAAQQAMDDLRAGRFQGAAVLLAE"
     gene            complement(2367..2669)
                     /locus_tag="PK242_10485"
     CDS             complement(2367..2669)
                     /locus_tag="PK242_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOZ94489.1"
                     /translation="MSSIQLHEDPRDLSDATRNMHRAIVSLMEELEAVDWYNQRADVC
                     TDPELKAVLEHNRDEEIEHASMTLEWIRRNNRVFDKELREFLFTDQPLSKAADHEH"
     gene            2726..3061
                     /locus_tag="PK242_10490"
     CDS             2726..3061
                     /locus_tag="PK242_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020830850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOZ94490.1"
                     /translation="MNPALDDPDLRALLRATLAATPAGGAVICLCAAWCGTCRAYEPG
                     FRQAAAAHPALAFRWLDVEDDADALGDLDIETFPTLVIGGADGAVRFAGPVLPQPAQI
                     TRLLHSLGL"
     gene            3143..4096
                     /gene="ybiB"
                     /locus_tag="PK242_10495"
     CDS             3143..4096
                     /gene="ybiB"
                     /locus_tag="PK242_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HOZ94491.1"
                     /translation="MSICSYIKVIGRAAPGAQPLEREQAADAMGQILDGTCTDLEIGA
                     FCLAMRIKGETPDELAGFLDAVQARMTRLPASDRPVVVLPSYNGARRLPVLTPLLALM
                     LARKGLPVLLHGSPTEDKRVTSLDVLAALGIGTHAPDERIPSGRVHVVPTERLLPGLK
                     RLLDVRRAIGLRNAGHALVKLLNPIDGPALVVSSYTHPEYAATMASVFDRFDTTALLL
                     RGTEGEPVADARRTPRMVLVSNGQARCVQEAQQGALTTLPRVPTLIDAATTADFSRAV
                     MEGRLPAPSPIVLQVEHIVRACKMLEASYDRTLLQPLVSQI"
     gene            4093..4869
                     /gene="cobA"
                     /locus_tag="PK242_10500"
     CDS             4093..4869
                     /gene="cobA"
                     /locus_tag="PK242_10500"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOZ94492.1"
                     /translation="MSASSTGRVTLVGAGPGDPELLTLKAVRAIQAASVILVDDLVGD
                     GVLAHARPDARITYVGKRGGCKSTPQATIEQLMLQAARAGENVVRLKGGDPFIFGRGG
                     EEVESLRRAGIEPEVISGITAGLAGLTSLGAPLTHREHAQGVVFVTGHPQKDGPGIDW
                     ARLAASARDLRLTLVIYMGISSAARIQAGLLTALPADTPVAVIERASLPAQRQCITRL
                     GELLTELHHSGLGSPAVVVVGDVVRGVAAVHQQPTQLAIA"
     gene            4881..>6146
                     /locus_tag="PK242_10505"
     CDS             4881..>6146
                     /locus_tag="PK242_10505"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOZ94493.1"
                     /translation="MAAAGAFDAVILGAGAAGLFCAARAGQRGLRVLLIDHAARLAEK
                     VRISGGGRANFTNRDLDPKAPQRHYLSANPRFCRSALARFAPADFIALLDRHGIGWHE
                     KHRGQLFCDRSARQLIDMLLAECAAASAHGGAVERWQPCRVERVEFIAKSGSGAHPKS
                     AASYIIHSERGVVQSPALVVASGGLSIPRIGASGLGLALARQFGLPIVPTRPGLVPLT
                     FDAAAWAPYAPLAGLSLPVRMATGVKAQRIEFQEDLLFTHRGLSGPAVLQISSYWQPG
                     TPITIDLAPGIDLAAALAQAKARSRRWIANELAQWLPARLADTWCARQAAWQRPINEA
                     GDKALAQLAEHLQRWELTPTGTEGYAKAEVMVGGVDTRALSSQTLEAQAQPGLYFIGE
                     VVDVTGWLGGYNFQWAWASAAACADALAAA"
BASE COUNT          852 a         2259 c         2113 g          922 t
ORIGIN      
        1 ccaggcattc ctgcaccgtc tggtgcgggt tgaccacgta caggcgcgtg gtcatcacgt
       61 cggccagcag ggtgtcggcc gaggccagac ccttgagcac caggcggcgc gcgtagtcgc
      121 gctcggtgaa gatgcccagc agcgcgtcac cctgcacgat gagcaccgag ccgatgttgc
      181 ggtcggccat gatctgcagc gcctcgcgta cggtggcggt gggcggcagc gttgccagtg
      241 tcgtgccctg gcgcgatttc agaatctcgg caacggtatg catggcgctt ctcctgcaaa
      301 gactgggatg aacacgtcga tacggtcatt ttgccgactt tggcgcagtt gggaagggcc
      361 tgcgtcaaat tctgtgccga cgccgcggcg gcgccacatc gggctcggat ttggcggcac
      421 aatggccctg actgtactcg catgcgctat gtgtttgata gcttatggcg cttgatggtc
      481 atgccttgat ggccgattca tttgatttcc ccgacccccg cgtgacatga ccgccgccct
      541 caccgtgatt tcctccatgg ccacccgcaa gctgctgggc gagttgatcg acaagctggc
      601 cggcgcgccg gtgacggtgg agtcggtcgg cggcgtcgac gcggcgcgac gcgtggccgc
      661 cggcgaggtg ttcgatgccg tggtgctggg tgccgacgcc atcgaccggc tcatcgcgga
      721 cggccaggtg cgcgccggca gccgggtcga cctggtgcgc tccggcgtgg cgctggcggt
      781 aaaagccggc gcgccacggc ccgacatcgg cagcgcccag gcggtgcggg ccgccgtgct
      841 ggcggcgcgc accgtgggct gttccaccgg acccagcggc gtggcgctgg cccggctgtt
      901 cgaccactgg ggcatcgccg agcaggtgca ggcccggctg gtggtgccgc cgcccggcac
      961 gccggtgggc gcgctgatcg cccgcggcga ggtggaactg ggtttccagc agctcagcga
     1021 actgatgttc ctgcccggca tcgacgtgct cggcccgctg cccgaaccga tccagatcac
     1081 caccgtattt gccggcggcg tgcccaccgc gtcgcgccag ccgcaggccg cacaggcgct
     1141 gctggagcgg ctggcagcgc ccgacttgac cgccatcaag cgcgccaacg gcatggacag
     1201 cgcctgaccc acagccccgc atgaacgaac ccaccgaaac cagcctggcc atggtgttgc
     1261 gcggcgcgcg ccaggcgctg caggtcgagc gccgcccgct gccccagccc ggcgccggcc
     1321 aggtgcgcct gcgggtgcgc gcatgcgccg tctgccgcac cgacctgcac ctgcaggatg
     1381 gtgacctgcc gatggcgcgc tggccggtgg tgcccggcca cgaagtggtc ggcaccgtcg
     1441 acgccctggg cgccgccacc accggactgc gcctgggcca gcgcgtcggc gtgccctggc
     1501 tgggcggcgc ctgcggccag tgccgctact gcctggcggg gcaggaaaac ctgtgcgacg
     1561 cgccccagtt caccggctgc acgcgcgacg gcggctttgc cacccacatc gtggccgacg
     1621 ccagcttctg cgtgccgctc gaccacctgg cgctggacga cgccagcgcc gccccgctgc
     1681 tgtgtgccgg tctgatcggc tggcgctcgc tgcgcctggc cggtgacgat gcgcgcgtgc
     1741 tgggcctgta cggcttcggc gccgccgccc atctgatcgc ccaggtggcg ctggcgcagg
     1801 gtcggcgcgt acacgccttc acccgccccg gcgacacggc ggcgcagcag cacgcacgcg
     1861 ccctgggctg cacctgggcc ggcgacgccg accagcccgc ccccgagccg ctggacgcgg
     1921 ccatcctgtt tgccccggca ggcgagttgg tgccgctggc gttgcgggcg gtgcgcaagg
     1981 gcggacgcgt ggtgtgcggc ggcatccaca tgagcgacat ccccagcttt ccctatgccc
     2041 tgctgtggca ggagcggcaa ctgctgtcgg tggccaacct gacgcgcgcc gacgcacgcg
     2101 agttctttgc cgcactgcag gccctgccgc cgctgcgcat acacaccacg ccctacccgc
     2161 tgcaggccgc ccagcaagcc atggacgacc tgcgggccgg gcgatttcag ggagcggcgg
     2221 tgctgctggc ggagtgacga tgccggcgca agcggacacc gcctgccgcc aagatcttac
     2281 aaacaattcg gctctggcgc gcttgtatca agcgccagca gctcttgttt ttatagcttg
     2341 ccagggttcg atgggcgaca ctcagtctag tgctcgtggt cggccgcctt gctgagcggc
     2401 tgatcggtga acaggaattc ccgcagttcc ttgtcgaaca cgcggttgtt ccgacggatc
     2461 cactccagcg tcatgctggc gtgctcgatc tcctcgtcgc ggttgtgctc cagcaccgcc
     2521 ttcaattcgg ggtcggtgca gacgtcggcg cgctggttgt accagtccac cgcttccagc
     2581 tcctccatca gcgaaacgat ggcgcggtgc atgttgcggg tggcgtcgga cagatcacgg
     2641 gggtcttcgt gcaactggat gctcgacatg gcgctgactc tcctcggtcg ggtgggacaa
     2701 tgcttgtgcg ccccactgta acccgatgaa cccggccctg gacgatcccg acctgcgcgc
     2761 cctgctgcgt gccacgctgg cagccacccc ggccggcggt gcggtgatct gcctgtgcgc
     2821 cgcctggtgc ggcacctgcc gcgcgtacga gcccggcttc cggcaggcgg cggcggcgca
     2881 tcccgcgctg gcgttccgct ggctcgacgt ggaggacgac gccgacgcgc tgggcgatct
     2941 ggacatcgaa accttcccca ccctggtcat cggtggcgcc gacggcgccg tgcgcttcgc
     3001 cgggccggtg ctgccccagc cggcacagat cacacggctg ctgcacagcc tgggcctgtg
     3061 agtccgacgg cccgcgcagg ccgatcaatt ccttgaccat ggtcaattga gtcctggccg
     3121 cccgcgccgc acaatgcctc ccatgtccat ctgttcgtac atcaaggtca tcggtcgcgc
     3181 cgcccccggc gcccagccgc tggaacgcga gcaggcggcc gacgcgatgg gccagatcct
     3241 cgatggcacc tgcaccgacc tcgaaatcgg cgccttctgc ctggccatgc gcatcaaggg
     3301 tgaaacgccc gatgagctgg ccggattcct ggacgccgtg caggcgcgca tgacccgcct
     3361 gcccgccagc gaccgcccgg tggtggtgct gcccagctac aacggggcgc gccggctgcc
     3421 ggtgctcacg ccgctgctgg cgctgatgct ggcgcgcaag ggcctgccgg tgctgctgca
     3481 cggcagcccg accgaagaca agcgcgtgac cagcctggac gtgctggccg ccctgggcat
     3541 cggcacccac gcccccgacg agcgcatccc cagcgggcgg gtgcatgtcg tgcccaccga
     3601 gcgcctgctg cccggcctca agcgcctgct ggacgtgcgg cgcgccattg gcctgcgcaa
     3661 cgccgggcac gccctggtca agctgctcaa cccgatcgac gggccggccc tggtggtatc
     3721 cagctacacc caccccgaat acgccgccac catggcctcg gtgttcgacc gcttcgacac
     3781 caccgccctg ctgctgcgcg gcaccgaggg cgaaccggtg gccgacgcac gccgcacgcc
     3841 acgcatggtg ctggtcagca acggccaggc gcgctgcgtg caggaagcgc agcagggtgc
     3901 gctgaccacg ctgccgcggg tgccgacgct gatcgacgcc gcaaccaccg ccgacttcag
     3961 ccgcgccgtg atggaaggcc gcctgccggc gcccagcccc atcgtcttgc aggtcgaaca
     4021 catcgtgcgc gcctgcaaga tgctcgaagc atcgtacgat cggacgctgc tgcaacccct
     4081 ggtgtcccag atatgagtgc ttcttccact ggccgcgtca cgctcgtggg cgccggcccg
     4141 ggcgaccccg aactgctgac actcaaggcc gtgcgcgcca tccaggccgc cagcgtgatc
     4201 ctggtcgacg acctggtggg tgacggcgtg ctggcgcacg cccggcccga cgcccgcatc
     4261 acctatgtcg gcaagcgcgg cggctgcaag agcacgcccc aggccaccat cgagcagctg
     4321 atgctgcagg ccgcgcgcgc cggcgaaaac gtggtgcgcc tgaagggcgg cgaccccttc
     4381 atcttcggtc gcggcggcga agaggtcgaa agtctgcgcc gcgccggcat cgagccagag
     4441 gtgattagcg gcatcaccgc cggcctggcc gggctgacca gcctgggcgc gccactcacc
     4501 caccgcgagc acgcgcaggg tgtggtgttc gtcaccggcc acccgcagaa ggacggcccg
     4561 ggcatcgatt gggcgcgact ggccgcatcg gcgcgcgacc tgcgcctgac gctggtgatc
     4621 tacatgggca tcagcagcgc cgcgcgcatt caggccgggc tgctgacagc cttgcccgcc
     4681 gacaccccgg tggccgtgat cgagcgcgcc agcctgcccg cccagcgcca gtgcatcacc
     4741 cgcctgggcg agctgctcac cgagctgcac cacagcggcc tgggcagccc ggccgtggtg
     4801 gtggtgggcg acgtggtgcg cggcgtggcc gccgtccacc agcagccgac ccagctcgcc
     4861 atcgcctgag cagccccggc atggctgcgg cgggcgcctt cgacgccgtc atcctgggcg
     4921 ccggcgccgc cggcctgttc tgcgccgccc gcgctggcca gcgcgggctg cgcgtgctgc
     4981 tgatcgacca tgccgcgcgg ctggccgaaa aggtgcgcat ctccggcggc gggcgcgcca
     5041 acttcaccaa ccgcgacctc gaccccaagg cgccgcaacg ccactacctg agcgccaacc
     5101 cgcgcttctg tcgctcggcg ctggcgcgtt ttgcgccggc agacttcatc gccctgctcg
     5161 atcggcacgg catcggctgg cacgagaagc accgcggcca gctgttctgc gaccgctcgg
     5221 cgcgccagct gatcgacatg ctgctggccg aatgcgccgc cgcctcagcc cacggcggcg
     5281 cggtcgagcg ctggcagccg tgccgcgtcg agcgcgtcga attcatagcc aaatcaggtt
     5341 ctggcgcgca cccaaaaagc gccgcaagct acattattca tagtgaacgc ggggtcgtgc
     5401 aaagcccggc gctggtggtc gccagcggcg ggctgtcgat cccccgcatc ggcgccagcg
     5461 gcctgggcct ggcgctggcg cgccagttcg ggctgcccat cgtgccgacc cgcccggggc
     5521 tggtgccgct gaccttcgac gccgccgcct gggcgcccta cgcgccgctg gccgggctgt
     5581 cgctgccggt gcgcatggcc accggcgtca aggcgcagcg catcgaattc caggaagacc
     5641 tgctgttcac ccaccgcggc ctgtcgggcc cggcggtgct gcagatctcc agctactggc
     5701 agcccggcac ccccatcacc atcgatctgg cgcccggcat cgacctggcc gccgccctgg
     5761 cgcaggccaa ggcgcgttcg cgccggtgga tcgccaacga gctggcgcag tggctgccgg
     5821 cccgcctggc cgatacctgg tgcgcccggc aagcggcctg gcagcgcccc atcaacgagg
     5881 ccggcgacaa ggcgctggcg caactggccg aacacctgca gcgctgggag ctgacgccca
     5941 ccggcaccga aggctatgcc aaggccgagg tcatggtggg cggcgtcgac acccgggcgc
     6001 tgtcgtcgca aacgctggag gcgcaagccc agccgggcct gtacttcatc ggcgaggtgg
     6061 tcgacgtgac cggctggctg ggcggctaca acttccagtg ggcctgggcc agcgccgccg
     6121 cctgcgccga cgcgcttgcc gctgca
//
LOCUS       DAOOOG010000151         6142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_15259_length_6142_cov_30.540825, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000151 DAOOOG010000000
VERSION     DAOOOG010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15259_length_6142_cov_30.540825"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1790)
                     /locus_tag="PK242_10510"
     CDS             complement(207..1790)
                     /locus_tag="PK242_10510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOZ94494.1"
                     /translation="MDSRPQPTDRATLLARLAEPCKYDLAVVGGGATGLGVALDAAQR
                     GHRVVLVEAHDFAKGTSSRATKLVHGGVRYLAQGNVHLVREALHERVHILQSAPHLAQ
                     PLPFVMPSYRLWETPFYGVGLKMYDLLAGRAGLGATQWLGARETLRLLPGAQPQGLKG
                     GVKYWDGQFDDARLALALARTAARHGALLVNHCEAIDVLHDAGRAVGLVVRDAESGRE
                     LRLQAGCIVNATGVWVDGLRQHDARAGGQPVQPVVAPSQGAHLVVEQSFSPGDHALLV
                     PSTTDGRVLFAVPWLGKLILGTTDHPRPDVPLEPQAGADEVDFILREAGRYLRRAPTR
                     ADILSRWAGLRPLVRPADAGSDTKAISREHTILVSPSGLVSVTGGKWTTYRAMAEDVL
                     ARCTEAGVLLPRGPSRTAGLPLVGAVGAGRRTPLSAPPGLHLYGSEAETVAALPGADR
                     WLAPGLSEAMVRFAVRHEYARTVEDVLARRSRLLFLDAAEAARAAPAVARVLGEERAG
                     DPALAAFQALAGVYAGQGA"
     gene            complement(1862..3415)
                     /gene="glpK"
                     /locus_tag="PK242_10515"
     CDS             complement(1862..3415)
                     /gene="glpK"
                     /locus_tag="PK242_10515"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOZ94495.1"
                     /translation="MRYVLALDQGTTSSRAIAFDEQGRVAALAQREFAQHFPQPGWVE
                     HDADEIWVSQLAVARDCIQKLAAAQHGQAPKAIEIVAIGITNQRETTLLWERATGRPL
                     APAIVWQDRRTTARCEQLRSQGQAGPIQRQTGLVLDAYFSATKLEWLLDQVPGARARA
                     EAGELAFGTIDSWLIWQLTGGRVHATDPSNAARTMLMNIHTLDWDDQLLRLFHIPRAV
                     LPRIVPSSGVLGETAPVLFGRPIPIAGVAGDQQAATFGQACFAPGMAKNTYGTGCFML
                     MNTGHQAVPSRNRLLTTVGWQGSAAGARHRTAYCLEGSVFMAGATVQWLRDGLQMIQT
                     ASEIEALAASVPDTGDVFLVPAFAGLGAPDWDGRARGTLVGLTRGSTRAHIARAALEA
                     IALQSADIFGAMSRDSGIALTELRVDGGASRNDLLMQIQADLLGVPVVRPRVTETTAL
                     GAAYLAGLATGVWPDGAAIAAQWAVARRFEPRLPEPERHARIARWRQAVARARGWARD
                     AAGSSPSQP"
     gene            3519..4352
                     /gene="proC"
                     /locus_tag="PK242_10520"
     CDS             3519..4352
                     /gene="proC"
                     /locus_tag="PK242_10520"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOZ94496.1"
                     /translation="MTATALPASTRIAFIGGGNMASAIIGGLIRQGLPAARIDVVEPF
                     EATRAALLQQHGVTALPAAGPQLAGADLIVWAVKPQSFGAAAAPVAPHVGSALQLSVM
                     AGIRSGDIAAASGARRIVRCMPNTPALVGRGMTGLFAAPEVSAADRALAEAVIRTTGE
                     ALWLEHEAQLDAVTALSGSGPAYVFYFLEAMQQAGSELGLSPEQARRLAVATFAGGAH
                     LAAASSEPLATLRERVTSKGGTTYAALTALDQAGVKPAFVAAMHAACARAKELGDEFG
                     R"
     gene            4567..5361
                     /locus_tag="PK242_10525"
     CDS             4567..5361
                     /locus_tag="PK242_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HOZ94497.1"
                     /translation="MPTLSRRQFAALGLVGLTIPPVWAQVKDMNDAINKAGRQRMLSQ
                     RVAKAYLGVALEALPEQAEKVLSQSMALFDRQLVELKGFAPNPGIRDTYGQLERAWLD
                     FKDRLVGKPASMAGADAVLAQSETVLALAHQGTTQLEKASGQPSGRLVNLAGRQRMLS
                     QRLAKYALASGARIQLAAATTEIEKNRSEFVTGLEVLAEAPQATPRIRETLKLGEQQW
                     LLFDAAIRSSSRSGQPVKEALAHIMTTSENLLLVLDDVTNQYAALR"
     gene            complement(5370..>6142)
                     /locus_tag="PK242_10530"
     CDS             complement(5370..>6142)
                     /locus_tag="PK242_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HOZ94498.1"
                     /translation="LSALWLAAGGFPGWHLLAVFVAGTILMRSAGCCVNDVADREFDR
                     HVKRTAQRPVTTGAVGVREALALGAVLALAAFVLVLTTNASAIALSFPALAIAIAYPF
                     AKRWVSMPQAVLGVAFSFGIPMAFAAVQGQVPWQAWALLAGNLFWVLAYDTEYAMVDR
                     DDDLKIGIQTSAITLGRWDVAAIMGFYAAYLSIWIGIVLQQAPTAIVYIAFGLAAGQA
                     LWHYTLIRGRTREGCFTAFRQNHWLGATVFAGIALALR"
BASE COUNT          909 a         2209 c         2181 g          843 t
ORIGIN      
        1 ctcgagtcga gattcagtct ggatcatttg taagtttccc aacttgcatc cggccgcctc
       61 tgacgtgaag ccgacaccga aacagtcttg ggcccgccac ccaggccgcg cgctcttttc
      121 atgcgcgcat gcgccaggcg atgtggggca gattcctcac ccaataaggg cgaagcccgc
      181 cattgtggca aagcgagctg gctttgtcaa gcgccctggc cggcgtagac cccggccagg
      241 gcctggaatg ccgccagcgc ggggtcgccc gcccgttctt cgcccagcac gcgcgccacg
      301 gcgggggcgg cgcgcgcagc ctcggcggcg tccagaaaca acaggcgcga gcggcgcgcc
      361 agcacgtcct cgacggtgcg ggcgtattcg tggcggacgg caaaacggac catggcctcg
      421 ctcaggccgg gcgccagcca gcgatcggcg ccgggcaggg ccgccacggt ctcggcctcg
      481 ctgccgtaca ggtgcaggcc gggcggcgcg ctcagggggg tgcgccggcc ggcgccgacg
      541 gcgcccacca ggggcagccc ggcggtgcgc gagggaccgc gcggcagcag cacgccggct
      601 tcggtgcagc gcgccagcac gtcttcggcc atggcgcggt aggtggtcca tttgccgccg
      661 gtgacgctga ccaggccgga gggcgacacc aggatggtgt gctcgcggct gatggccttg
      721 gtgtcgctgc cggcgtcggc cggccgcacc agcgggcgca ggccggccca gcggctgagg
      781 atgtcggcgc gggtgggcgc gcggcgcagg tagcggccgg cttcgcgcag gatgaaatcg
      841 acctcgtcgg cgccggcttg cggctccagt ggcacgtcgg ggcgcgggtg gtcggtggtg
      901 cccaggatca gcttgcccag ccagggcacg gcaaacagca cccggccgtc ggtggtgctg
      961 ggcaccagca aggcgtgatc gccgggcgaa aacgactgtt ccaccaccag gtgggcgccc
     1021 tggctgggcg ccaccaccgg ctgcaccggc tggccgccgg cgcgcgcgtc gtgctggcgc
     1081 agcccgtcca cccagacgcc ggtggcgttg acgatgcagc cggcctgcag gcgcagttcg
     1141 cgcccgcttt cggcgtcacg caccaccagg ccgacggcgc ggccggcgtc gtgcagcacg
     1201 tcgatcgcct cgcagtggtt gaccagcagc gcaccgtgcc gcgccgccgt gcgtgccagc
     1261 gccagcgcca gacgggcgtc gtcgaactgg ccgtcccagt atttgacgcc gcccttcaga
     1321 ccctgcggct gcgcccccgg cagcaggcgc agcgtctcgc gcgcgcccag ccattgggtg
     1381 gcgcccagac cggcacggcc ggccagcagg tcgtacatct tcaggccgac gccgtagaac
     1441 ggcgtttccc acagccggta cgacggcatg acgaagggca gcggctgcgc caggtgcggc
     1501 gcgctctgca ggatgtgcac gcgttcgtgc agcgcctcgc gcaccaggtg cacgttgccc
     1561 tgcgccaggt agcgcacgcc gccatgcacc agcttggtgg cgcgcgaaga ggtgcccttg
     1621 gcgaagtcgt gcgcctccac cagcaccacg cggtggccgc gctgcgcggc gtccagcgcc
     1681 acccccaggc cggtggcgcc gccgcccacc acggccaggt cgtacttgca gggctcggcc
     1741 agacgggcca gcagggtggc gcggtcggtc ggctgggggc ggctgtccat ggctcgaaat
     1801 tactattgtt ttgatagcta gcggcgcttg ctggataagc gctgaatgcc gatgcaatgc
     1861 ctcatggctg cgacggcgat gacccggcgg cgtcgcgtgc ccagccgcgc gcgcgcgcca
     1921 ccgcctggcg ccagcgggcg atgcgggcgt gccgctcggg ctcgggcagc cgcggctcga
     1981 agcgtcgcgc caccgcccac tgcgccgcga tcgccgcgcc gtcgggccag acgccggtgg
     2041 ccagcccggc caggtaggcg gcgcccaggg cggtggtttc ggtgacgcgc gggcgcacca
     2101 ccggcacgcc cagcagatcg gcctggatct gcatcagcag gtcgttgcgg ctggcgccgc
     2161 cgtccacgcg caactcggtc agggcgatgc cgctgtcgcg gctcatggcg ccgaagatgt
     2221 cggccgactg cagggcaatg gcttccagcg cggcgcgggc gatgtgcgcg cgcgtgctgc
     2281 cgcgcgtcag ccccaccagg gtgccgcgcg cgcggccgtc ccagtcgggc gcgcccaggc
     2341 cggcgaaggc gggcaccagg aacacgtcgc cggtgtcggg cacgctggcc gccagcgcct
     2401 cgatctcgct ggcggtctgg atcatctgca ggccgtcgcg cagccattgc acggtggcgc
     2461 cggccatgaa caccgagcct tccaggcaat aggccgtgcg gtggcgcgcg cccgccgccg
     2521 agccctgcca gcccacggtg gtgagcaacc ggttgcggct gggcacggcc tggtggccgg
     2581 tgttcatcag catgaagcag ccggtgccgt aggtgttctt ggccatgccg ggggcaaagc
     2641 aggcctgccc gaaggtggcg gcctgctggt cgccggccac gccggcgatc gggatcgggc
     2701 ggccgaacag gaccggcgcc gtctcgccca gcacgccgct ggacggcacg atgcgcggca
     2761 gcacggcgcg cgggatgtga aacagccgca gcagttggtc gtcccagtcc agcgtgtgga
     2821 tgttcatcag catcgtgcgg gcggcgttgc tggggtcggt ggcgtgcacg cggccgccgg
     2881 tcagctgcca gatcagccag ctgtcgatgg tgccgaaggc cagctcgccg gcttcggcgc
     2941 gggcgcgtgc gccgggcacc tggtcgagca gccattcgag cttggtggcc gagaaatacg
     3001 catccagcac cagcccggtc tgacgctgga tcggcccggc ctggccctgg ctgcgcagct
     3061 gctcgcagcg cgccgtggtg cgccggtcct gccagacgat ggcgggcgcc agtggacgac
     3121 ccgtggcgcg ctcccacagc agcgtggttt cgcgctggtt ggtgatgccg atagctacaa
     3181 tttcaatagc ttttggcgct tgcccatgct gcgctgcagc cagtttttgt atgcaatctc
     3241 gggccaccgc cagctggctg acccagatct cgtccgcatc gtgttcgacc cagccgggct
     3301 gcggaaaatg ctgcgcgaat tcgcgctgcg ccagcgccgc cacccggccc tgctcgtcaa
     3361 acgcgatggc gcggctgctg gtggtgccct gatcgagtgc gagaacgtag cgcatgggtg
     3421 caagggtagc ggattcggct gcgcccgggt cgcccgccct gcgccagggc gcgcttacac
     3481 tgaccgccct ccgtcacccg ccacgccgcc cgaccgccat gaccgccacc gccctgcccg
     3541 cctccacccg catcgccttc atcggcggcg gcaacatggc cagcgccatc atcggcgggc
     3601 tgatccggca ggggctgccg gcggcgcgca tcgacgtggt ggagcccttc gaggccacgc
     3661 gcgccgccct gttgcagcag cacggcgtga ccgcactgcc cgccgccggc ccccagctcg
     3721 ccggcgccga cctgatcgtc tgggccgtca aaccgcagag cttcggtgcc gccgcggcgc
     3781 cggtggcacc acacgtgggc agcgcgctgc agctgtcggt gatggccggc atccgcagcg
     3841 gcgacatcgc cgccgccagc ggcgcgcggc gcatcgtgcg ctgcatgccc aacaccccgg
     3901 ccctggtcgg gcgcggcatg acggggctgt ttgccgcgcc cgaggtgagc gcggccgacc
     3961 gcgcgctggc cgaagccgtg atccgcacca ccggcgaggc gctgtggctg gagcacgagg
     4021 cgcagctcga cgccgtcacc gccctgtccg gctccggccc ggcctatgtg ttctatttcc
     4081 tcgaagccat gcagcaggcg ggcagcgagc tgggcctgtc gcccgaacag gcgcgccgcc
     4141 tggccgtggc cacctttgcc ggcggcgcgc acctggcggc cgcctcgagc gagccgctgg
     4201 ccaccctgcg cgagcgcgtg accagcaagg gcggcaccac ctacgccgcc ctgaccgcgc
     4261 tggaccaggc cggcgtcaag cccgcgttcg tcgccgccat gcacgccgcc tgtgcgcgcg
     4321 ccaaggaact gggcgacgag ttcgggcgct gagcgcattg cctttcaacc gggtcggccg
     4381 gggcctaccc tgctgccggc gcgccatgca cccgaaacct gaagccaggt caaggttgcc
     4441 tcgtgtactt tgatattact atttcgccct gatcctgcga gtggcaggtg ttgaccgggt
     4501 tgacgcgggt gcgcggccac tggaaggaca caccctgacc ccgccgcagg aaatggagtg
     4561 ccgctcatgc caacgctttc ccgccgtcag ttcgccgccc tggggttggt cggcctgacc
     4621 atcccgcccg tttgggccca ggtcaaggac atgaacgacg ccatcaacaa ggccggtcgc
     4681 cagcgcatgc tgtcgcagcg cgtggccaag gcctatctgg gtgtggcgct ggaggcgctg
     4741 cccgagcagg cggaaaaggt gctgagccag tcgatggcgc tgtttgaccg ccaactggtc
     4801 gaactcaagg gtttcgcccc caacccgggc atccgcgaca cctacggcca actcgaacgc
     4861 gcctggctgg atttcaagga caggctggtc ggcaagcccg cctcgatggc gggcgccgac
     4921 gccgtgctgg cacagtccga aaccgtgctg gcgctggcac accagggcac cacgcaactg
     4981 gagaaggcat ccggccagcc gtcagggcgc ctggtcaacc tggccgggcg ccagcgcatg
     5041 ctgtcccagc ggctggccaa gtacgcgctg gccagcgggg cgcgcatcca gctggcagcc
     5101 gccaccacgg agatcgaaaa gaaccgcagc gagttcgtga ccggcctcga ggtcctggcc
     5161 gaggcgcccc aggccacgcc gcgcatccgc gagacgctga aactgggcga gcagcagtgg
     5221 ctgttgttcg atgccgccat caggtcgtcc agccgcagcg gccagccggt caaggaagca
     5281 ttggcgcaca tcatgaccac cagcgagaac ctgctgctgg tgctggacga tgtgaccaac
     5341 cagtacgccg cgctgcgctg aaaccaccct catcgcagcg ccagcgcgat gccggcgaac
     5401 accgtggcgc ccagccagtg gttctgccgg aaggccgtga agcagccttc acgggtgcgc
     5461 ccgcggatca gtgtgtagtg ccacagcgcc tgccccgcgg ccagcccgaa cgctatgtaa
     5521 acaatagctg tcggcgcttg ctgcaaaacg atcccaatcc agatcgacag gtaagcggcg
     5581 taaaacccca tgatcgccgc cacatcccag cggcccaggg tgatggccga ggtctggatg
     5641 ccgatcttca agtcgtcgtc gcggtcgacc atggcgtatt cggtgtcgta ggccagcacc
     5701 cagaacaggt tgcccgccag cagcgcccag gcctgccacg gcacctggcc ctgcaccgcc
     5761 gcaaaggcca tcgggatgcc gaagctgaag gccacgccca gcaccgcctg cggcatcgac
     5821 acccagcgct tggcaaacgg gtaggcgatg gcgatggcca gcgccggaaa cgacagcgca
     5881 atggcgctgg cgttggtggt cagcaccagc acaaaggccg ccagcgccag caccgcgccc
     5941 agcgccagcg cctcgcgcac gccgaccgca ccggtggtga ccgggcgctg cgcggtgcgc
     6001 ttgacgtggc ggtcgaactc gcggtcggcc acgtcgttga cgcagcagcc ggccgagcgc
     6061 atcaagatgg tgccggcgac gaacaccgcc agcaggtgcc agcccggaaa gccgccggcc
     6121 gccagccaca gcgccgacag gg
//
LOCUS       DAOOOG010000152         6007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_15743_length_6007_cov_27.059980, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000152 DAOOOG010000000
VERSION     DAOOOG010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15743_length_6007_cov_27.059980"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1682
                     /locus_tag="PK242_10535"
     CDS             141..1682
                     /locus_tag="PK242_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOZ94499.1"
                     /translation="MPDLLPFDRPAPLFDLAATRRIEATAQAALPPHTLMQRAGLATA
                     RLALALAPHARTVWVACGPGNNGGDGLEAAMHLQGWGLHPAVTWLGDEAHLPADARAS
                     LQRARAAGVGFADAPPPLGPHDLAIDALLGIGATRAPSGRMADWLRQLRHGPATLLAL
                     DVPSGLDADTGCLHDSDATHSIAVGASSACAGGRFDTQPPHGARHTLSLLTRKPGLFT
                     AQGRDACGTLWFDDLGVDPGEVAPTAWLNAPPALAPRPHDSHKGRFGDVAVIGGASDT
                     AQGTAMTGAALLAAAAALHAGAGRVYVGPLGATAPTFDPVRPELMFRPPDRLPLAQLT
                     VVCGCGGGDAVRAVLPAVLAQAPRLVLDADALNAIAQDAALQQALAARAGRGQSSVLT
                     PHPLEAARLLGGDVRQVQADRLRAAQALAGRLESTVVLKGSGSVIAAPGQTPRLNATG
                     NARLATPGSGDVLAGLIGARLAGGRPALEAACDAVHQHGALADAWPAHTALTAGALAQ
                     RLTPA"
     gene            1711..2379
                     /locus_tag="PK242_10540"
     CDS             1711..2379
                     /locus_tag="PK242_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ94500.1"
                     /translation="MRILLAEDEPELASWLARALERGGLLADWVSDGRDVLPSLAARR
                     YDALVLDLGLPGLDGHQVLAALRATDQRLPVLILTARDSLIERVGTLNQGADDFLAKP
                     FDLAELEARLHALIRRARGSAHWRQSCGPLAWDATTRGFTLNEAPLTLTPREHAVLRV
                     LIQHVGEPLSKAEILDRVVSDERDVNPEAIEVLVHRLRKRLAGSQVRIGTLRGLGYVL
                     EHGA"
     gene            2376..3827
                     /locus_tag="PK242_10545"
     CDS             2376..3827
                     /locus_tag="PK242_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HOZ94501.1"
                     /translation="MRLGWLRASLRRALALVLAAGMLLASGVGLWGAWRSAAGAANAA
                     YDRSLAGAIKAIDANITTAGGGLGVELPYTLLEFFQLTAGGQVFFRVATEDGLVEIGN
                     TDLPPPDTGLINGRPRFRDALYHGQPVRLGSYTRVLDPPPAGHTGRPRVVIQVAETLT
                     SRQDFTRALLLEAVGRNALLVTLALLLMAWATGWALRPLARVSREVQARPPDDLTPLP
                     LADVPAEARPLVEAMNHHMARHRQASEARRRFVDDASHQLRTPLTTLSTQLAYALREA
                     PPGPLREALTAMRAQLDGATRQVNQMLALARADAAEPRAEVVDLVALARELTRHGWPR
                     AREAGIDLGFESQAEQLPASVQPILLQEALGNLLHNALRYCPAGSRVTVRVAQEGGQA
                     RLTVLDDGPGLAPDELARAGERFFRGRQAAAGGTGLGLAIARAVAERHGGTLSLQTGA
                     GGCGLAVSLLLPLRELPTSPGFPQVETAPLKAD"
     gene            3915..4895
                     /locus_tag="PK242_10550"
     CDS             3915..4895
                     /locus_tag="PK242_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ94502.1"
                     /translation="MKFRLPLAALTLACGLGLAHAGPLDKTECIAPAKPGGGFDLTCK
                     LAQSSLLNGKYISDPMRVTYMPGGIGAVAYNAVVAQRPAEGNTIVAFSGGSLLNLAQG
                     KFGRYGENDVRWLAAVGTDYGAVIVAEASPFKTLNDLVAAIKADPTKVVFGAGGTVGS
                     QDWMKAALTAKAAGLDPRKMRFVAFEGGGEASTALQGGHVQVYSGDASEAEEQIKAGA
                     KIRVLAVMADKRLEGGLAKVPTAKEQGLDVEWPIIRGYYMGPKVSDADFKVWTDTFSK
                     MMATPEFAKARAERSLYPFAKTGADLDAYVKQRVADYRKLAAEFGLNVTK"
     gene            4951..5397
                     /locus_tag="PK242_10555"
     CDS             4951..5397
                     /locus_tag="PK242_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOZ94503.1"
                     /translation="MSDRILGVACVVVAAGMAWAAKDYAAPISYEPVGPRAFPLLLAT
                     LMAAGGLWLIVRPNLRGNWLRQVPLRSLAFAVAAVFAYALLFELLGFTLATVVMAVPV
                     GMAFGGSWLQALAGGAGLGLLLYLGFDKLLDVVLPTGLLSFILGGR"
     gene            5401..>6007
                     /locus_tag="PK242_10560"
     CDS             5401..>6007
                     /locus_tag="PK242_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOZ94504.1"
                     /translation="METLQYLMTGFGVALSTTNLLVAAIGALVGTIVGMLPGLGPING
                     VAILMPLAFALKLPAETALILLAAVYMGCEFGGRISSILLNVPGDAGAIMTALDGYPM
                     ARQGMGGVALSISAWSSFIGSLIATVGIVMFAPLLARWALAFGPAEYFALMCFAFACI
                     TGLMGDAPMKAALAAVIGLSLACVGLDSNSGVYRFTGGDVHL"
BASE COUNT          780 a         2232 c         2157 g          838 t
ORIGIN      
        1 tgggcgctgt cctgcggctc ggcgcgcgtg ctggccgacc agatcgccgg ccgcgtgccc
       61 gacatcgaca ccgaaggcct ggacgtgcag cgctggctgc gttgacggtg gcgggcggca
      121 ccggagccac ataatgcccc atgcccgacc tgctgccctt cgatcgcccc gccccgctgt
      181 tcgacctggc cgccacccga cgcatcgaag ccacggcgca agccgcgctg ccgccgcata
      241 ccctgatgca gcgcgccggc ctggccacgg cacggctggc gctggcgctg gcgccgcacg
      301 cgcgcacggt gtgggtcgcc tgcggcccgg gcaacaacgg cggcgacggc ctggaagccg
      361 ccatgcacct gcagggctgg ggcctgcacc cggcggtcac ctggctgggc gacgaagccc
      421 atctgccggc cgatgcgcgc gcctcgctgc agcgcgcgcg cgccgccggc gttggcttcg
      481 ccgatgcgcc gccacccctg ggaccgcacg acctggcgat cgacgccctg ctgggcatcg
      541 gcgccacacg cgcgccgagc gggcgcatgg ccgactggct gcggcaactg cgccacggcc
      601 cggccaccct gctggcgctc gacgtgccca gcgggctgga cgccgacacc ggctgcctgc
      661 atgactcgga tgctactcat tcgatagctg tcggcgcttc atccgcctgc gctggaggcc
      721 gatttgatac acaaccgccg cacggtgcgc ggcacacgct cagcctgctg acgcgcaagc
      781 ccggtctgtt caccgcccag ggccgcgacg cctgcggcac gctgtggttc gacgatctcg
      841 gcgtcgatcc aggcgaggtc gcgcccaccg cctggctgaa cgccccgccg gcgctggcac
      901 cgcgtccgca cgacagccac aagggccgct ttggcgatgt ggcggtgatc ggcggcgcca
      961 gcgacaccgc gcagggcacg gccatgaccg gcgcggcgct gctggcggcg gcggccgcgc
     1021 tgcatgccgg tgccggccgg gtgtacgtcg gcccgctggg agcgacggcg ccgaccttcg
     1081 atccggtgcg gccggagctg atgttccgcc cccccgaccg cctgccgctg gcgcagctga
     1141 ccgtggtgtg cggctgcggc ggcggcgacg cggtgcgcgc cgtgctgccc gccgtgctgg
     1201 cgcaggcgcc gaggctggtg ctggacgccg acgcgctgaa cgccatcgcg caggacgcgg
     1261 cattgcagca ggcgctggcc gcgcgcgccg ggcgtggtca gtccagcgtg ctcacaccgc
     1321 acccgctgga ggcggcgcgc ctgctgggcg gcgacgtgcg tcaggtccag gccgaccgcc
     1381 tgcgcgctgc gcaggccctg gccgggcgcc tggaatccac cgtggtgctc aaaggctcgg
     1441 gcagcgtcat cgccgcgccg ggccagacgc cgcgcctgaa cgccaccggc aacgcccgcc
     1501 tggccacgcc tggctcgggc gacgtgctgg ccggactgat cggcgcgcgg ctggcgggcg
     1561 gccggccggc gctggaggcc gcctgcgacg ccgtccacca gcatggcgcg ctggccgacg
     1621 cctggcccgc gcacaccgcc ctcaccgccg gcgcgctggc gcagcgcctg acgccggcgt
     1681 gaccgccgcc cccccacccg gacaccgacc atgcgcatcc tgctcgccga agacgaaccc
     1741 gagctggcaa gctggctggc gcgtgcgctg gagcgtggtg gcctgctggc cgactgggtg
     1801 agcgatggcc gcgacgtgct gcccagcctg gcggcacgcc gctacgacgc gctggtgctc
     1861 gatctgggcc tgcccgggct ggacgggcac caggtgctgg ccgccctgcg cgccaccgac
     1921 cagcgcctgc cggtgctgat cctgacggca cgcgactcgc tgatcgagcg cgtcggcacg
     1981 ctcaaccagg gcgccgacga cttcctggcc aagccgttcg acctggcgga gctggaagcg
     2041 cgcctgcacg cgctgatccg ccgtgcgcgc ggcagcgccc actggcgcca gagctgcggc
     2101 ccactggcct gggacgccac cacccgcggc ttcaccctga acgaggcgcc gctgacgctg
     2161 acgccgcgcg agcacgccgt gctgcgcgtg ctgatccagc acgtgggcga gccgctgtcg
     2221 aaggcggaaa tcctcgaccg cgtggtgtcg gacgagcgcg acgtcaaccc cgaggcgatc
     2281 gaggtgctgg tgcaccgcct gcgcaagcgc ctggcgggca gccaggtgcg catcggcacc
     2341 ctgcgcgggc tgggctacgt gctggagcat ggcgcatgag gctcggctgg ctgcgcgcca
     2401 gcctgcgccg agcgctggcg ctggtgctgg cggccggcat gttgctggcc tcgggcgtgg
     2461 gcctgtgggg cgcctggcgc agcgccgccg gcgccgccaa cgccgcctac gaccgctcgc
     2521 tggccggtgc catcaaggcc atcgacgcca acatcaccac cgccggcggt ggcctggggg
     2581 tggagctgcc ctacaccctg ctggagttct tccagctcac cgccggcggt caggtgttct
     2641 tccgtgtcgc caccgaagac gggctggtcg agatcggcaa caccgacctg ccgccgccgg
     2701 acaccggtct gatcaacggc cggccgcgct ttcgtgacgc gctctaccac ggccagccgg
     2761 tgcggctggg ctcgtacacg cgggtgctcg atccgccccc ggccggtcac accgggcgac
     2821 cgcgcgtggt gatccaggtg gccgagaccc tgacgtcgcg ccaggacttc acgcgcgcgc
     2881 tgctgctcga agcggtgggg cgcaacgcgc tgctggtgac gctggcgctg ctgctgatgg
     2941 cctgggccac cggctgggcg ctgcgcccgc tggcgcgggt gtcgcgcgag gtgcaggcgc
     3001 gcccgcccga cgacctgacg ccgctgccgc tggcggacgt gccggccgaa gcgcgtccgc
     3061 tggtggaagc catgaaccac catatggcac gccatcgaca ggccagcgag gcgcgccgac
     3121 gcttcgtcga cgacgcctcg caccagctgc gcacgccgct gacgacgctg tcgacgcagc
     3181 tcgcctacgc gctgcgcgaa gcgccacccg gccccctgcg cgaagccctg acggcaatgc
     3241 gcgcgcagct cgacggtgcc acccggcagg tcaaccagat gctggcgctg gcgcgcgccg
     3301 atgcggccga gccgcgcgcc gaagtggtcg acctggtggc gctggcgcgc gagctgacgc
     3361 gccatggctg gccgcgggca cgcgaggctg gcatcgacct cggcttcgaa agccaagccg
     3421 agcagctgcc ggcgtcagta cagcccatac tgctgcagga agcgctgggc aacctgctgc
     3481 acaacgcgct gcgctactgc ccggccggca gccgcgtcac ggtgcgggtg gcgcaggagg
     3541 gcggacaggc gcgcctcacc gtgctggacg acggccccgg cctggcaccc gatgaactgg
     3601 cgcgcgcggg cgagcgcttc tttcgcggcc gccaggcagc ggccggcggc accggcctgg
     3661 gtctggccat tgcccgcgcc gtggccgagc gccatggcgg caccctctcg ctgcagaccg
     3721 gagcgggtgg ttgcgggctg gcggtcagcc tgctcctgcc cctccgggag ttgcccacat
     3781 ccccgggttt tccccaggtg gagacggccc cattgaaagc cgattgaaag tcggcaggcg
     3841 ctagagtgac ctgtacgggg cgccgcgccg tcggctcccg acccgatccg tcaccacgtt
     3901 caggagactg cgccatgaaa tttcgactcc cccttgccgc cctcaccctg gcctgcggcc
     3961 ttggcctggc ccacgccggc ccgctggaca agaccgaatg catcgccccg gccaagcccg
     4021 gcggcggctt cgatctgacc tgcaagctgg cccagtccag cctgctcaac ggcaagtaca
     4081 tcagcgaccc gatgcgcgtg acctacatgc cgggcggcat cggcgccgtg gcctacaacg
     4141 ccgtggtggc ccagcgcccg gccgaaggca acaccatcgt cgccttctcg ggcggctcgc
     4201 tgctcaacct ggcgcagggc aagttcggcc gctacggcga aaacgacgtg cgctggctgg
     4261 ccgccgtcgg caccgactac ggcgccgtca tcgtggccga ggcgtcgccc ttcaagacgc
     4321 tcaacgatct ggtggccgcc atcaaggccg acccgaccaa ggtggtgttc ggcgccggcg
     4381 gcacggtggg cagccaggac tggatgaagg ccgcgctgac cgccaaggcc gccggcctgg
     4441 acccgcgcaa gatgcgcttc gtcgccttcg agggcggcgg cgaagcctcg accgccctgc
     4501 agggcggcca cgtgcaggtg tattcgggcg acgcctccga ggccgaggag cagatcaagg
     4561 ccggcgccaa gatccgcgtg ctggcggtga tggccgacaa gcgcctggaa ggcggcctgg
     4621 ccaaggtgcc caccgccaag gagcaaggac tggatgtgga atggcccatc atccgcggct
     4681 actacatggg cccgaaggtg agcgatgccg acttcaaggt ctggaccgac accttcagca
     4741 agatgatggc cacgcccgaa ttcgccaagg cacgcgccga gcgcagcctc tacccgttcg
     4801 ccaagaccgg cgcggacctg gacgcctacg tcaagcagcg cgtggccgac taccgcaagc
     4861 tggcggccga attcggcctg aacgtcacca aatgaggcgg cgcgccgcgc cgggacgccg
     4921 gccggcgcat ccaccggagg agcatcaacc atgagcgacc gcattctggg cgtcgcctgc
     4981 gtcgtggtgg cggccggcat ggcctgggcc gccaaggact acgcggcgcc gatttcctac
     5041 gagccggttg gcccgcgtgc gttcccgctg ctgctggcca ccctgatggc cgccggcggc
     5101 ctgtggctga tcgtgcggcc caacctgcgc ggcaactggc tgcgccaggt gccgctgcgc
     5161 tcgctggcgt ttgccgtcgc cgccgtgttc gcctacgcgc tgctgttcga gctgctgggc
     5221 ttcaccctgg ccacggtggt gatggcggta ccggtcggca tggccttcgg tggcagctgg
     5281 ctgcaggcgc tggccggtgg cgccggcctg ggcctgctgc tgtacctggg cttcgacaag
     5341 ctgctggacg tggtgctgcc caccgggctg ctgtcgttca tcctcggggg gcgctgaacc
     5401 atggaaaccc tgcaatacct gatgaccggc ttcggcgtcg ctctgtcgac caccaacctg
     5461 ctggtggccg ccatcggcgc gctggtgggc accatcgtcg gcatgctgcc agggttgggg
     5521 ccgatcaacg gcgtggccat cctgatgccg ctggcgttcg cgctcaagct gccggccgag
     5581 acggcgctga tcctgctggc cgcggtctac atgggctgcg agttcggtgg ccgcatctcc
     5641 tccatcctgc tgaacgtgcc gggcgacgcc ggcgccatca tgacggcgct ggacggctac
     5701 cccatggcgc gccagggcat gggcggcgtg gcgctgtcga tttcggcctg gagctcgttc
     5761 atcggctcgc tgatcgccac cgtcggcatc gtgatgttcg cgccgctgct ggcgcgctgg
     5821 gcactggcct tcggcccggc cgagtacttc gccctgatgt gcttcgcctt cgcctgcatc
     5881 accggcctga tgggcgacgc cccgatgaag gcggcgctgg cggcggtgat cggcctgtcg
     5941 ctggcctgcg tcgggctgga ctccaactcc ggcgtctacc gcttcaccgg cggcgacgtg
     6001 cacctgt
//
LOCUS       DAOOOG010000153         5992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_15801_length_5992_cov_26.153950, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000153 DAOOOG010000000
VERSION     DAOOOG010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15801_length_5992_cov_26.153950"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PK242_10565"
     CDS             <1..848
                     /locus_tag="PK242_10565"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206863.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOZ94505.1"
                     /translation="RLEFIINNNIGVHPKAILDYPQVDADLKKAVESVARGHASPRAF
                     YVDKVAEGVATIAAAFWPKPVIVRLSDFKSNEYRKLIGGSRYEPEEENPMLGFRGAAR
                     YISDDFREAFAMECEALRRVRDDMGLTNVQIMVPFVRTLAQAERVVALLADKGLRRGV
                     NDLKVVMMCEVPSNAILAERFLEHFDGFSIGSNDLTQLTLGLDRDSGLELLAADFDER
                     DPAVEALIHQAIQACRAQGKYIGICGQGPSDHPDFARWLADEGIVSISLNPDSVIDTW
                     TLLSQ"
     gene            870..3185
                     /locus_tag="PK242_10570"
     CDS             870..3185
                     /locus_tag="PK242_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HOZ94506.1"
                     /translation="MSAPPAIKNEAPGAAWLRAALLLLWAAASFGVAFFARELNQVVA
                     GWPLNYWWLAQGGVLVFLGVVMLYAWWMGRAEPAPADEGATSVAAGSRAYIWRLHRQL
                     ALFTLGLVAFLLLLHWAERAGLSRGWIGPIFLFLSVMMYAAVGIAGRTTDPEEFYVAG
                     RRVPAMYNGMATAADWMSAASFISLAGALYLQGFSGTAAQPGGLAYVLGWTGGFCLVG
                     LLMAPRLRAMRLYTVPDFFAVRFGRGAQLLAAMGAVACSFMYVVAQIYGIGLIASRLT
                     GVQFEIGILLGLGGVLLCSFLGGMRAITWTQVVQYVVLLVAFLIPVSWLAYKQLGTPV
                     ALLAYGQQLSKIERLERTLIDAPAEQQVRQHYRERARDYAERLRDPAAALASERSALE
                     ERIRTLRAQGADFSFVMMARRELSALPKDEASARELWTRAMLDAQERAKPLGGLPRHG
                     QAFAGDPDGTPDERAEFNTSRLNFLALMFCLMVGTASLPHLLTRFYTTPSVAATRHSV
                     AWSLLFIAMLYLATPALAALVKYEVMQHLVGSSFDDLPNWIAQWARVDPALISVQDVN
                     GDRILQFGEIRLGADMIMLATPELGGLPYVVSGLVAAGGLAAALSTADGLLLTISNAL
                     VHDTAPMRGGRVWVSDNRVILSKFALLAVAMVAAATAALKTGEIISLVTASFSLAAAT
                     FFPALTLGLFWRGATRRAAVAGMLTGIAVTVGYMLQASAVLRAWWGLAPGGLWWGIHP
                     VSAGFFGVPAGFAVIAAVSAWEHWRGRRQRP"
     gene            complement(3320..3892)
                     /locus_tag="PK242_10575"
     CDS             complement(3320..3892)
                     /locus_tag="PK242_10575"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOZ94507.1"
                     /translation="MHRHALTARDFAADAPQVARTLIGATLLVDGVGGIIVETEAYDR
                     ADAASHTHRGPTPRNAAMFGPPGHAYVYRSYGIHWCLNLVCREAGHGAGVLIRALQPT
                     HGLDTMRRRRGLQDERLLCAGPGRVGQALGITLAHYGLAVDAPPFELRAAPHGLADQV
                     VCGPRIGISRALELPWRFGLAGSRYLSRRF"
     gene            4228..5268
                     /locus_tag="PK242_10580"
     CDS             4228..5268
                     /locus_tag="PK242_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94508.1"
                     /translation="MAKGCLTLTGLFGLALVLERLALGERGMPLPWLAAALLAASLTL
                     ALGSVQGVAQALRRRNAPESEPSRWRDGESVRTGGRIEPSGPLLRTPLGGCDAVLVEY
                     SIYRVPSQARIGGNPQARPTPPSAKGLEMVPFRVRNAAGAIAIDGWASLAQFPEADCD
                     HAAGREGAARVLASEPWRVSTPDEAVRAGRELLGGPAGTPLALRLANPAAVRELLAPE
                     GEPNAGRWSGGPPPDLEGEAAVAAVRARLARHRWLLKERRLAPGDEVTVEGIYRADPP
                     RIEVGRGPSLGAPERGIRPGLPDVTARREAHTALAFALALAAVAAAAHWLVFSSGGQF
                     YRGLVDWLRQAA"
     gene            5511..5992
                     /locus_tag="PK242_10585"
                     /pseudo
     CDS             5511..5992
                     /locus_tag="PK242_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          850 a         2022 c         2149 g          971 t
ORIGIN      
        1 cgcggctgga gttcatcatc aacaacaaca tcggcgtgca ccccaaggcc atcctcgact
       61 acccgcaggt cgacgccgac ctgaagaagg ccgtggagag cgtggcgcgt ggccacgcca
      121 gcccgcgcgc cttctacgtc gacaaggtgg ccgagggcgt ggcgacgatt gccgccgcct
      181 tctggcccaa gccggtgatc gtgcgccttt cggacttcaa gagcaatgaa taccgcaagc
      241 tgatcggcgg cagccgctac gagccggaag aagaaaaccc gatgctcggc tttcgcggtg
      301 ccgcgcgcta catcagcgac gatttccgcg aagccttcgc catggaatgc gaggcgctgc
      361 gccgcgtgcg cgacgacatg ggcctgacca acgtgcagat catggtgccc ttcgtgcgca
      421 cgctggcgca ggccgagcgg gtggtggcgc tgctggccga caaggggctg cggcgcggtg
      481 tcaacgacct caaggtggtg atgatgtgcg aggtgcccag caacgccatc ctggccgagc
      541 gcttcctgga gcatttcgac ggcttttcca tcggctcgaa cgacctgacc cagctcacac
      601 tggggctgga tcgcgattcc ggtctggaac tgctggcggc cgacttcgac gagcgcgatc
      661 cggccgtgga ggcgctgatt caccaggcca tccaggcctg ccgggcgcag ggcaagtaca
      721 tcggcatctg cggtcagggc cccagcgacc acccggactt cgcgcgctgg ctggccgacg
      781 agggtatcgt ctcgatttcg ctcaaccccg acagcgtgat cgacacctgg accttgctgt
      841 cacaataaaa aacccgtcca tcgcacgcca tgtcagcgcc accagctatc aaaaatgaag
      901 ctcctggcgc ggcctggctg cgtgccgcgc tgctgctgtt gtgggcggcc gcgtcgttcg
      961 gtgtggcctt tttcgcgcgc gagctgaacc aggtcgtggc cggctggccg ctcaattact
     1021 ggtggctggc gcagggcggg gtgctggtgt tcctcggcgt ggtgatgctg tatgcgtggt
     1081 ggatgggccg cgccgagccc gcgcctgccg acgagggggc gactagcgtg gctgccgggt
     1141 cgcgcgccta catctggcgg ttgcatcgtc agctggcgct gttcaccctg ggcctggttg
     1201 cctttctgct gctgctgcac tgggccgagc gcgccggtct gtcgcgcggc tggattgggc
     1261 cgatcttcct gttcctgtcg gtgatgatgt acgcggcggt gggcattgcc gggcgcacca
     1321 ccgacccgga ggagttctac gtcgccggcc ggcgcgtgcc ggccatgtac aacggcatgg
     1381 ccacggcggc cgactggatg agcgccgcct ccttcatcag cctggccggg gcgctgtacc
     1441 tgcagggctt ttctggaacc gccgcgcagc ccggtggcct ggcctacgtg cttggctgga
     1501 ccgggggctt ttgtttggtc ggattgctga tggcgccgcg cctgcgcgcc atgcggctgt
     1561 acaccgtgcc cgacttcttt gccgtgcgat tcggtcgggg ggcgcaactg ctggcggcga
     1621 tgggggcggt ggcctgctcg ttcatgtacg tggtggcgca gatctacggc atcggtctga
     1681 tcgcctcgcg cctgaccggg gtgcagttcg agatcggcat cctgctgggc ctgggcgggg
     1741 tgctgctgtg ctccttcctg ggcggcatgc gcgccatcac ctggacacag gtggtgcagt
     1801 acgtggtgct gctggtggcc tttctgatcc cggtgtcctg gttggcgtac aagcagctgg
     1861 gcacgcccgt ggccttgctg gcgtacggcc agcagctgtc caagatcgag cggctggagc
     1921 gcacgctgat cgatgcaccc gccgagcaac aggtgcgcca gcactaccgc gagcgcgccc
     1981 gcgactacgc cgagcgcctg cgcgatccgg cggccgcgct ggccagcgag cgcagcgcgc
     2041 tggaggagcg catccgcacg ctgcgcgccc agggtgcgga cttttccttc gtcatgatgg
     2101 cccggcgcga gctgtcggcc ctgcccaagg acgaggccag cgcgcgcgag ctgtggacgc
     2161 gcgccatgct cgacgcgcag gagcgcgcca aaccgctggg tgggctgccg cgacacggtc
     2221 aggcgtttgc cggcgacccc gacggcacac cggacgagcg cgccgaattc aacacctcgc
     2281 gcctgaactt cctggccctg atgttctgcc tgatggtggg taccgccagc ctgccgcacc
     2341 tgctgacgcg cttctacacc acccccagcg tggcggcgac gcggcactcg gtggcctggt
     2401 cgctgctgtt cattgccatg ctgtacctgg ccacaccagc gctggcggcg ctggtcaagt
     2461 acgaggtgat gcagcatctg gtgggcagca gcttcgacga tctgcccaac tggattgccc
     2521 agtgggcgcg ggtggatccg gcgctgattt cggtgcagga cgtcaacggt gaccgcatcc
     2581 tgcagttcgg cgaaatccgc ctgggcgccg atatgatcat gctggccacg cccgagctgg
     2641 gtgggctgcc ctacgtggtg tccggcctgg tggcggccgg cgggctggcg gcggcgctgt
     2701 ccaccgccga cggactgctg ctgaccatca gcaatgcgct ggtgcacgac accgcgccca
     2761 tgcgcggcgg ccgcgtgtgg gtgtcggaca accgcgtgat cctgtccaag ttcgccctgc
     2821 tggcggtggc catggtggcg gcggcgacgg cggcgctcaa gacgggcgag atcatttctc
     2881 tggtgacggc ctcgttttcg ctggcggccg ccaccttctt cccggcgctc accctcggcc
     2941 tgttctggcg cggcgccacc cggcgcgccg ccgtcgccgg catgctgacc ggcatcgccg
     3001 tcaccgtcgg ctacatgctg caggccagcg cggtcctgcg cgcctggtgg ggcctggcgc
     3061 cgggcggtct gtggtggggc atccacccgg tgtcggccgg cttcttcggc gtgccggcag
     3121 gttttgccgt gatcgccgcg gtgtcggcct gggagcactg gcgcgggcgg cgccagcgcc
     3181 cctgatcgag cgccgcaaag ctctcgattg ctattaataa cgtagcttac ggcgctgact
     3241 gggtgcgcgc tggagcatgt tttttcctca aacccgccag aatccaggtt ggggccggga
     3301 gcgccggtcg agggcctgat cagaaccttc ggctcaggta gcgcgagccg gccaggccaa
     3361 atcgccacgg cagctccagc gcccggctga tgccgatgcg cgggccgcac accacctgat
     3421 ccgccagccc gtggggtgcg gcccgcagct cgaacggcgg cgcatccacc gccagcccgt
     3481 agtgcgccag cgtgatgccc agcgcctgtc ccacacgacc ggggccggcg cacagcaggc
     3541 gttcgtcctg caggccgcga cgccggcgca tggtgtccag gccgtgcgtc ggctgcaggg
     3601 cgcggatcag cacgccggcg ccgtgtccgg cttcgcggca caccaggttc aggcaccagt
     3661 ggatgccgta gctgcggtac acgtaggcat ggccgggcgg gccgaacatg gcggcgttgc
     3721 gtggtgtcgg gccgcggtgg gtgtgcgagg cggcgtcggc gcggtcgtag gcctcggtct
     3781 cgacgatgat gccgcccacg ccgtccacca gcagagtggc gccgatcagg gtgcgggcaa
     3841 cctgcggtgc atcggcggcg aagtcgcggg cggtgagggc gtggcggtgc atgacggtcg
     3901 ccagtctatg gattcggcgt tggcatgtct tcaataccgt tcgggctgag cctgctcaag
     3961 cccttcgaca ggctcaggat gcacggttca aatttcagga attcggaagc catagggtct
     4021 gctcacaggc cctggtgcag ggtggcgctg cgcagggtga cgggctgaga tgcacgcggt
     4081 gctcagcgct gcaccgcagg cagccgagtg ggcgcagtat gctgagggac tgcagatcgc
     4141 gccccacccc gcggtgtctt ttggagctgg aagccactcg actcaactcc gcgccccggg
     4201 cttcccgcac gaataggaag gagatcgatg gcaaagggct gcctcacgtt gaccggactc
     4261 ttcgggctcg cgctcgtcct cgagcgcttg gcgctcgggg agcgcggcat gccgttgccc
     4321 tggctcgccg ccgcgctgct cgccgcgagc ctgacgctgg cgctcggaag tgtccagggg
     4381 gtcgcgcagg ccctgcgacg tcggaacgcg ccggagagcg aaccctcgcg gtggagggac
     4441 ggcgagagcg tgcgcaccgg cggccggatc gagccgtccg gcccgctgct ccgcacacct
     4501 ctcggggggt gcgacgcagt gctcgtcgag tactcgatct accgggtgcc cagccaggcc
     4561 cggatcggag gaaacccgca ggcgcggccc accccaccga gtgccaaggg cctcgagatg
     4621 gtgccgttcc gggtgcgcaa tgccgccggt gcgatcgcca tcgacggctg ggcgagcctg
     4681 gcgcagtttc cggaggcaga ctgcgaccac gcagcgggga gggaaggagc ggcccgggtt
     4741 ctggcctccg agccctggcg cgtctcgacg ccggacgaag ccgtccgcgc cgggcgggaa
     4801 ctgctgggtg gaccggcggg aacgcccctt gccctccggc tcgcgaaccc cgcggcggtc
     4861 cgcgagctcc tggcgccgga gggagagccg aacgccggcc gctggtcggg cgggccgcct
     4921 cccgacctcg agggagaggc cgccgtagca gcggtccgcg cccgcctcgc gcggcacagg
     4981 tggctgctga aggagcgccg cctcgccccc ggggacgagg tcacggtgga ggggatctac
     5041 cgcgcggacc cgccgcggat cgaggtcggc cgcgggccgt ctctcggcgc gcccgagcgc
     5101 gggatccggc ccggtctgcc cgacgtcacc gcccggcggg aggcacacac ggcgttggcc
     5161 ttcgccctcg cccttgcggc ggtggcggcc gcggcccact ggctcgtctt ctcctccggc
     5221 gggcagttct accgcgggct cgtcgactgg ctccgccagg ccgcctgacc cgccggccgc
     5281 gagcggggcg agaggcagga ggcggaggcg accgcgcgag cgccccaggc gaacgcctcg
     5341 tcgttgagcg ggatcatcgc gcacttctcc ttcagggagc gccgaatcgc gtggaggaac
     5401 gtctccttgg ggaagactgc gtcgcctgct gcagcgctcc gagcgcgacc aggttcttcg
     5461 ccagcgtgcg gccgagatcg gcggcgatct gcgcgaacgc cacaccgtac gcggtcatgc
     5521 ggcattggca cgcgcacggc ctcaagcctc acgtcctgcg cggcttcaag gcctcgcgcg
     5581 atcccaagtt cgtggacaag ctcgaagaca tcgtggcgca gtacatgccc ctgcccgagc
     5641 atgcgctggt gctgtgctgc gacgagaaga gccaggtgca ggcgctggac cgcacgcaac
     5701 cagggttgcc actgaagagg ggatgtgcgc agaccatgac gcacgacgac aagcgccatg
     5761 gcacgaccac gctgtttgca gcgctgaacg tcctggacgg acaggtcatt ggtcggtgcc
     5821 tgcagcggca cacccacgtc cagtggctga agttcctgcg tcaaatcgac cgcgaaacac
     5881 ccaaggacaa gacactgcac ctgatcgccg acaacgacgc tacgcacaag cacccagtcg
     5941 tacagcagtg gctggccatg agcggctcag ctgccgttcg ggcaccgtcc gc
//
LOCUS       DAOOOG010000154         5976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_15855_length_5976_cov_27.873332, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000154 DAOOOG010000000
VERSION     DAOOOG010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15855_length_5976_cov_27.873332"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /gene="dcd"
                     /locus_tag="PK242_10590"
     CDS             <1..140
                     /gene="dcd"
                     /locus_tag="PK242_10590"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632155.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOZ94509.1"
                     /translation="LPAKIYAGEGCAQVLFFESDEVCEVSYKDRGGKYQGQVGVTLPK
                     T"
     gene            complement(144..1049)
                     /locus_tag="PK242_10595"
     CDS             complement(144..1049)
                     /locus_tag="PK242_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta(1)-pyrroline-2-carboxylate reductase
                     family protein"
                     /protein_id="HOZ94510.1"
                     /translation="MLLNPLDSAARLPWAALVDEIEALLGDPAVRVPERIILPTAQGA
                     VLFVMPACDAGIAMTKLISFTPANVGTARPTIQGDVTVFDVATGARRLILDGPTVTGR
                     RTAAVSALAARRLAPNRAGPMLVVGAGVQGQAHVEAFAAALGVREFRLASRRRTSAEA
                     LAVHARALGLRAEVVEDADAALADCPLVATCTPAREVVLRAMPRADAFIAAVGAFTPQ
                     MVELDAALCRAIAAQGRIVIDTPDAAHEAGDLIQAGVPVQGLPTLAQMVAADETPARG
                     PVLFKNCGWAGWDLAAARLALRSEA"
     gene            complement(1055..1741)
                     /gene="pcp"
                     /locus_tag="PK242_10600"
     CDS             complement(1055..1741)
                     /gene="pcp"
                     /locus_tag="PK242_10600"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HOZ94511.1"
                     /translation="MVAPASTSRLTTASADRPVVLVTGFDPFGGDALNPSWLLAERLH
                     RRRVSGHVVQAAQLPTRFADSLPRLYALLAQWRPALVVCLGLAASRAALSIERVAINV
                     QDARIPDNAGAQPVDEPVVAGAPAAYFSTLPIKAMRRAMLAAGVPAEVSQTAGTFVCN
                     HVFYGLMHHLATRRGLQRTRGGFIHVPPLAEHGPQGLPLEQMVEGLRTGIRAALGTTG
                     DIRQGAGATD"
     gene            1782..2657
                     /locus_tag="PK242_10605"
     CDS             1782..2657
                     /locus_tag="PK242_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOZ94512.1"
                     /translation="MRWEGERQSDNVEDRRSGGGGGGFGIGGRGIGIGTIAIALLAWG
                     VFGINPLTTIGVLSGGGGAPTQVQQGPAKAPPADDRNAAFVSTVLASTEDVWGQIFQQ
                     GGARYQQPRLVLFRGATPTACGTGQSAMGPFYCPGDQKVYLDMNFFDTMTRQLGAPGE
                     FARAYVVAHEVGHHVQNLLGVTGKVDSMRGRISERQQNQLSVRLELQADCYAGIWANR
                     SQQAKQWLDADDIESAVNAAQQIGDDTLQKRGQGYAVPDSFTHGSSAQRVRWFMQGYK
                     TGSVQACDTFNAQGL"
     gene            2887..4323
                     /locus_tag="PK242_10610"
     CDS             2887..4323
                     /locus_tag="PK242_10610"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ94513.1"
                     /translation="MSTLYSDLALADPLKRAVADMGYETMTPIQAQAIPVVLAGQDVM
                     GAAQTGTGKTAAFSLPLLQRLMKHETASVSPARHPVRALVLLPTRELAVQVADQVKQY
                     AKYTQLRSACVFGGMDMKPQIAELRQGVEVLVATPGRLLDHIEAKTAVLNQVEYVVLD
                     EADRMLDIGFLPDLQRILSHLPKNRTTLLFSATFSPEIKRLAESYLSNPVLIEVARPN
                     ATASTVEQHFYLLHEDDKRRAIRQVLKDKDLRQAFVFCNSKLGTARLARALERDGLKT
                     TALHGDKSQDERLKSLEAFKQGEVDLLVATDVAARGLDIKDVPAVFNYDVPFNPEDYV
                     HRIGRTGRAGATGVAVTLVTRHDQRLMGELEKLLGRKIEVEPIEFEEERARGRHNDGR
                     RRWQADETGDARDQARRERRPAERRPAPVSAPADPFFDRPYEAPSEPPEQPPAWEQKA
                     RPSRPGLSANIKPKRKLAALFKAPEPTH"
     gene            complement(4395..5654)
                     /locus_tag="PK242_10615"
     CDS             complement(4395..5654)
                     /locus_tag="PK242_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HOZ94514.1"
                     /translation="MSAAPARPRDAAGMKRLALGLLLAAALLYALATALVPRHAGWGY
                     LAAFAEAAMVGAIADWFAVVALFRHPLGLPIPHTAIIAAGKDRIGENLASFIATHFLA
                     TPQVLDKLREFDAAGRLADWLAQPDHAERVARRLSAIGGWAVGALDDPRVRGFVADLA
                     RRGLQQIELTRLSGQLLQALTHQRRHQELLDGVLAQLARLLGQDRVQDAITETIAREI
                     RALKYVGLDQVAARLATRKVVVVVARTIIDMADDPAHQLRQRFDDMVDSCVSRLQDDP
                     VLQARGEQLKRDLLDHPALGAYVNRLWSELLAWLQADMGQEDSAIRHHIAQAARTLGE
                     RLAGDAAVRAWINEELQAQAPRLIERYREDIRRYIAARVRAWDAGEMTAELERHIGRD
                     LQFIRINGTLVGGLVGLAIHALTHLWR"
     gene            complement(5651..>5976)
                     /locus_tag="PK242_10620"
     CDS             complement(5651..>5976)
                     /locus_tag="PK242_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HOZ94515.1"
                     /translation="IVNALTRLRFCTPDGRMEFATKEGGAAAAPPGHLPWFDVPGRRT
                     AGEVVAFGHWSTLGWLDRPDLISLDTGCVWGGCLSAVRLGTDGRQRELIQVRCPQAQQ
                     PGGRA"
BASE COUNT          955 a         2159 c         2084 g          778 t
ORIGIN      
        1 cgctgccggc caagatctac gccggcgagg gctgcgccca ggtgctgttc ttcgaaagcg
       61 acgaggtgtg tgaagtcagc tacaaggacc gcggcggcaa gtaccagggc caggtcggcg
      121 tgacgctgcc caagacctga ggctcaagcc tcagagcgca gcgccagccg cgccgccgcc
      181 aggtcccagc cggcccagcc gcagttcttg aacagcaccg gcccgcgcgc cggtgtttcg
      241 tctgcggcca ccatctgcgc cagcgtgggc agaccctgca ccggcacgcc ggcctgaatc
      301 aggtcgccgg cctcgtgcgc ggcgtcgggc gtgtcgatga cgatgcggcc ctgcgcggcg
      361 atggcgcggc acagggcggc gtccagctcc accatctgcg gcgtgaaggc gccgacggcg
      421 gcgatgaagg cgtcggcgcg cggcatggcg cgcagcacca cctcgcgcgc cggcgtgcag
      481 gtggccacca gcgggcagtc ggccagcgcg gcgtcggcgt cttccaccac ctcggcgcgc
      541 aggcccagcg cacgcgcgtg gacggccagg gcctcggcgc tggtgcggcg gcgcgaggcc
      601 agtcggaact cgcgcacgcc cagcgcggcg gcaaaggcct cgacatgcgc ctgcccctgc
      661 acgccggcac cgaccaccag catcggcccg gcccggttcg gtgccaggcg gcgggccgcc
      721 agcgccgaca cggcggcggt gcgccggccg gtcaccgtcg gtccgtccag gatcagccga
      781 cgcgcgccgg tggcgacgtc gaacaccgtc acgtcgccct ggatggtggg gcgcgccgtg
      841 cccacgttgg cgggcgtgaa gctgatcagc ttggtcatgg cgatgccggc gtcgcaggcc
      901 ggcatgacga acagcacggc gccctgggcg gtcggcagga tgatgcgctc gggcacccgc
      961 accgccggat cgcccagcag cgcctcgatc tcatcgacca gcgccgccca gggcaggcgc
     1021 gccgcgctgt cgagcgggtt cagcagcatc ggggtcagtc cgtggcgccg gcaccctgac
     1081 ggatgtcgcc ggtggtgccc agcgccgccc ggatgccggt gcgcagcccc tccaccatct
     1141 gctccagcgg caggccctgg gggccgtgtt ctgccagcgg tggcacatga atgaagccgc
     1201 cgcgcgtgcg ctgcaggccg cgccgcgtcg ccaggtggtg catcaggcca tagaacacat
     1261 ggttgcagac gaaggtgccc gccgtctgcg acacctcggc cggcacgccg gcggccagca
     1321 tggcccggcg catggccttg atcggcaagg tgctgaaata ggccgccggc gcgcccgcca
     1381 ccaccggctc gtccaccggc tgcgcgccgg cgttgtcagg aatgcgcgca tcctgcacgt
     1441 tgatggccac gcgctcgatc gacagcgccg cgcggctggc cgccagcccc aggcagacca
     1501 ccagcgccgg gcgccactgg gccagcagcg cgtacaggcg cggcagcgag tcggcaaagc
     1561 gcgtcggcaa ctgcgccgcc tgcaccacat ggcccgagac gcggcgccgg tgcaggcgct
     1621 cggccagcag ccaactggga ttgagtgcgt ccccgccaaa cggatcgaag ccggtgacga
     1681 gcaccaccgg gcggtccgcc gaggccgtcg tgagccggga tgtcgaagct ggcgctacca
     1741 tggcggcaat ttagcgcaac tcgcgccatg gggagcggca catgagatgg gaaggtgagc
     1801 gtcagtccga caacgtggaa gatcgtcgca gcggtggcgg cggaggtggc ttcggcatcg
     1861 gcggccgcgg catcggcatc ggcaccatcg ccattgcgct gctggcctgg ggtgtgttcg
     1921 gcatcaaccc gctgacgacg atcggcgtac tgtccggcgg cggtggtgcg ccgacgcagg
     1981 tgcaacaggg gccggccaag gcgccgccgg ccgatgaccg caacgccgcc ttcgtgtcca
     2041 ccgtgctggc ctcgaccgag gatgtgtggg gccagatctt tcagcagggc ggcgcgcgct
     2101 atcagcagcc gcgcctggtg ctgtttcgcg gcgccacgcc cacggcctgt ggcaccggcc
     2161 agtcggccat ggggccgttc tactgcccgg gcgaccagaa ggtgtatctg gacatgaact
     2221 tcttcgacac catgacgcgc cagctcggcg cgccgggcga gtttgcgcgc gcctacgtgg
     2281 tggcgcacga ggtcggccac catgtgcaga acctgttggg cgtgaccggc aaggtcgatt
     2341 cgatgcgcgg tcgcatcagc gaacgacagc agaaccagct gtcggtgcgg ctggagctgc
     2401 aggccgactg ctacgccggc atctgggcca accgctcgca gcaggccaag cagtggctgg
     2461 acgcggacga catcgaatcg gccgtcaacg ccgcccagca gatcggcgac gacacgctgc
     2521 aaaagcgcgg ccagggctac gccgtgcccg acagcttcac gcacggctcc agcgcccagc
     2581 gcgtgcgctg gttcatgcag ggctacaaga ccggcagcgt gcaggcctgc gacaccttca
     2641 acgcgcaagg gctttaagcg caggggcgtc cgctcaaaca ggcaggcgac ggcagaagaa
     2701 ctgaaggatt tcgcagaaag cgcgcaagaa aaggaggatt ccacttctgc gtcttctgag
     2761 cgacctttgc gccctctgca ttcggcgctc ggtcttctcg cttcaccccc gcggccaagc
     2821 ttcggtaccc cccacaagcc accgttgcac gcgcgccacg caccggtgcg acaatacggg
     2881 gttcgcatga gcaccctcta ttcagatctc gcgctggccg atcccctcaa gcgcgccgtg
     2941 gccgacatgg gctacgagac catgaccccc atccaggccc aggccatccc cgtggtgctg
     3001 gcgggccagg acgtgatggg cgcggcgcag accggcaccg gcaagacggc ggcgttttcg
     3061 ctgccgctgc tgcagcgcct gatgaagcac gagaccgcct cggtctcgcc ggcgcgccac
     3121 ccggtgcgcg cgctggtgct gctgcccacg cgcgagctgg cggtgcaggt ggccgaccag
     3181 gtcaagcagt acgccaaata cacccagctg cgcagcgcct gcgtgtttgg cggcatggac
     3241 atgaagccgc agatcgccga actgcgccag ggcgtggagg tgctggtggc cacgccgggg
     3301 cgcctgctgg atcacatcga ggccaagacc gccgtgctga accaggtcga gtacgtggtg
     3361 ctggacgagg ccgatcgcat gctggacatc ggttttctgc ccgacctgca gcgcatcctg
     3421 agccacctgc cgaaaaaccg caccaccctg ctgttttccg ccaccttctc gcccgagatc
     3481 aagcgcctgg ccgaaagcta tctgagcaac ccggtgctga tcgaagtggc gcgccccaac
     3541 gccaccgcct ccacggtgga gcagcatttc tacctgctgc atgaagacga caagcgccgc
     3601 gccatccgcc aggtgctcaa ggacaaggac ctgcgccagg ccttcgtgtt ctgcaacagc
     3661 aagctgggca cggcgcggct ggcgcgcgcg ctggagcgcg acggcctgaa gaccaccgcc
     3721 ctgcacggcg acaagagcca ggacgaacgc ctcaaatcgc tcgaagcctt caagcagggc
     3781 gaggtcgatc tgctggtggc caccgacgtg gccgcgcgcg ggctggacat caaggatgtg
     3841 ccggcggtct tcaactacga cgtgcccttc aaccccgagg actacgtgca ccgcatcggc
     3901 cgcaccggcc gcgccggcgc cacgggcgtg gcggtgaccc tggtcaccag gcacgaccag
     3961 cgcctgatgg gcgagctgga aaagctgctg ggccgcaaga tcgaggtcga gccgatcgag
     4021 ttcgaggaag agcgcgcccg cggccgccac aacgacggcc gccgccgctg gcaggccgac
     4081 gagaccggcg acgcgcgcga ccaggcccgg cgcgagcgcc gtccggccga gcgtcgcccg
     4141 gcccccgtca gcgccccggc cgacccgttc ttcgaccgcc cctacgaagc cccgagcgag
     4201 ccgcccgagc agccgccggc ctgggagcag aaggcgcgcc ccagccgccc cggcctgtcg
     4261 gccaacatca agcccaagcg caagctggcg gcgctgttca aggcgccgga gccgacccac
     4321 tgagccgctt tgtagccaaa taggcctctg gcgcgcaccc agcaagcgcc aacagctatc
     4381 catttaatag caattcagcg ccacaaatgg gtcagggcgt ggatcgccag ccccaccaga
     4441 ccgcccacca gggtgccgtt gatgcggatg aactgcaggt cgcgcccgat gtggcgttcc
     4501 agctcggcgg tcatctcgcc cgcgtcccag gcccgcacgc gggcggcgat gtagcggcgg
     4561 atgtcctcgc ggtagcgctc gatcaggcgt ggcgcctgcg cctgcagctc ctcgttgatc
     4621 caggcccgca cggcggcgtc gcccgccagg cgctcgccca gcgtgcgcgc agcctgggcg
     4681 atgtggtgac ggatggctga atcttcctgc cccatgtcgg cctgcagcca ggccagcagc
     4741 tcgctccaca agcggttgac gtaggcgccc agggccgggt ggtcgagcag gtcgcgcttg
     4801 agctgctcgc cacgcgcctg cagcaccggg tcgtcctgca gccgcgacac gcagctgtcc
     4861 accatgtcgt caaagcgctg gcgcaactgg tgcgccgggt cgtcggccat gtcgatgatg
     4921 gtgcgcgcca ccaccaccac caccttgcgc gtggccaggc gcgcggccac ctgatccagc
     4981 ccgacgtact tgagcgcccg gatctcgcgt gcgatggttt cggtgatggc gtcctgcacc
     5041 cgatcctgcc ccagcagccg cgccagctgc gccagcacgc cgtcgagcag ctcctggtgg
     5101 cggcgctggt gggtcagggc ctgcagcagc tgcccggaca ggcgcgtcag ctcgatctgc
     5161 tgcaggccgc gccgcgccag gtcggccacg aaaccgcgca cgcgcggatc gtccagcgcg
     5221 cccacggccc agccgccgat ggcgctcaac cgccgtgcca cgcgctcggc atggtcgggc
     5281 tgggccagcc agtcggccag ccggccggcg gcgtcgaact cgcgcagctt gtccagcacc
     5341 tgcggcgtgg ccagaaaatg cgtggcaatg aagctggcca ggttctcgcc gatgcggtcc
     5401 ttgccggccg caatgatggc ggtgtgcggg atcggcaggc caagcgggtg gcgaaacagc
     5461 gccaccacgg caaaccagtc ggcaatcgcg cccaccatgg cggcttcggc gaacgccgcc
     5521 aggtagcccc agccggcatg gcgcggcacc agcgccgtgg ccagggcgta cagcagggcc
     5581 gcggccagca gcaggccgag cgccagccgc ttcatgccgg cggcgtcgcg cgggcgagcc
     5641 ggtgcggcgc tcacgcccgg ccacccggct gctgcgcctg cgggcagcgc acctgtatca
     5701 gctcgcgctg acgtccatcc gtccccaggc gcacggcact caggcagcca ccccagacgc
     5761 aaccggtgtc gagcgagatc agatccggcc ggtccagcca gcccagggtc gaccagtgac
     5821 cgaaggccac cacctcgccg gccgtgcggc gcccgggcac atcgaaccag ggcaggtggc
     5881 cgggaggtgc ggcggcggcg ccgccctcct tggtggcgaa ctccatgcga ccgtcgggcg
     5941 tgcaaaagcg cagccgcgtg agggcgttga cgatga
//
LOCUS       DAOOOG010000155         5952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_15944_length_5952_cov_22.686112, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000155 DAOOOG010000000
VERSION     DAOOOG010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15944_length_5952_cov_22.686112"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK242_10625"
     CDS             complement(<1..342)
                     /locus_tag="PK242_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94516.1"
                     /translation="MPTPPSPGHFEREHQHLEAQLHAHLLDVAGADFERARQRLQRWR
                     RALARHIAIENDRLLPHLPAGARWPARLYLAEHERIELLADEYAQRLDALLARPLRSQ
                     RARRQAVLTLLD"
     gene            complement(796..2226)
                     /locus_tag="PK242_10630"
     CDS             complement(796..2226)
                     /locus_tag="PK242_10630"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HOZ94517.1"
                     /translation="MTRTYAAEPFKIKVVEPIKMTTRAERAQYLAEAGYNTFLLRSDQ
                     CYIDLLTDSGTTAMSDYQWAGMMLGDEAYAGSKNFIHLQEVVREYYGFKHIVPTHQGR
                     GAENLLSRLCIQPGDYLPGNMYFTTTRAHQELNGATFVDIINDQAHDPRVELPFKGNV
                     DIAKLSRLIDEVGPERIPYVCVAVTVNLAGGQPVSMGNLRAVKELCARHGIKVMFDAT
                     RCVENAYFIQEREPGYADVSIRAILKEMMSHADGCTMSGKKDCLVNIGGFLCMNDDEL
                     YQRASELVVLFEGMPSYGGLAGRDMEAMARGIEESVDQHYISHRIGQCRYLADQLEAA
                     GIPIVKPVGGHAVFLDARAFLDHLPQDEFPAQTLAAQLYLESGVRSMERGIVSAGRNK
                     ETGDHHRPKLELVRLTLPRRVYTYAHLDVVARACKDLYARRREIQGMKMVYEPKFLRF
                     FTARFEPLPPTTAPAGTQAAPVLAHA"
     gene            complement(2570..2950)
                     /locus_tag="PK242_10635"
     CDS             complement(2570..2950)
                     /locus_tag="PK242_10635"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOZ94518.1"
                     /translation="MHHPFADLIDLKVEVQRAGHSQLSLTLAPQHLNPHGVVHGAVVF
                     ALADTGMGSALYPTLEPGQICATIDIAISYFRPVRAGRLVCLTEVVNRGRTVAHLSSR
                     VLAGEQLVASATGNYAIFVPREAG"
     gene            complement(3231..3368)
                     /locus_tag="PK242_10640"
                     /pseudo
     CDS             complement(3231..3368)
                     /locus_tag="PK242_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409385.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 family protein"
     gene            complement(3384..5120)
                     /gene="soxB"
                     /locus_tag="PK242_10645"
     CDS             complement(3384..5120)
                     /gene="soxB"
                     /locus_tag="PK242_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HOZ94519.1"
                     /translation="MNLSKREFLQVLSAASVAGMGLGRFAEASAQTAAEGLYDIPRFG
                     NVSFLHITDCHAQLRPIYFREPSVNLGFGDMKGRLPHLVGDQLLKAAGVPAGSASAHA
                     LTYLDFDRAAQRYGKVGGFAHLATLVKRLRASRPGSLLVDGGDTWQGSGTALWTNGQD
                     MVDACKLLGVDAMTGHFEFTLGMKRVQEIVEKDFAGKVDFVAQNIKTADFGDPVFKPY
                     VIRQMNGVPVAIVGQAFPYTPIANPRWMVAEWEFGIQDENMQKVVDEARAKGAQVVVV
                     VSHNGMDVDLKMASRVRGIDAILGGHTHDGMPVASIVKNAGGQTIVTNAGSNGKFLGV
                     LDFDVKGGKVADFRYKLLPVFANMLPADKDMDALISKIRAPYEAKLSEQLAITEGLLY
                     RRGNFNGTGDQLIVDALMEVQGADLAFSPGFRWGTSLLPGQPITREWVMDMTATTYSY
                     ATVTEMKGEMIKTVMEDVADNLFNPDPYYQQGGDMVRVGGLSYSFDPLAPMGKRVSDM
                     RLKGQLIDADKSYKVAGWAPVAEDAAKAGNPMVWDLVEKWLKARGGKVAPRQLNNPKL
                     VGGVLPNPGYAA"
     gene            complement(5182..5853)
                     /gene="soxX"
                     /locus_tag="PK242_10650"
     CDS             complement(5182..5853)
                     /gene="soxX"
                     /locus_tag="PK242_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HOZ94520.1"
                     /translation="MTHRTSILALSIAGALTLTGCAGGGSAGVPSAAELDTLTQQIVQ
                     RDFHDKGIVKVSQAFTDDETMRVCNAADVSGKPLDDATAKRIEALNMATIKWPANGKY
                     LGDWKEGEKLAQSGRGLTWTDKADTPNGGNCYNCHQIDKKEISFGTIGTSLYNYGKLR
                     GVTDPNSPQAQEMVKYTWGKIWNSKAYNACSNMPRAGHKGILTEKQVADLVALLLDPN
                     SPVNK"
BASE COUNT          833 a         1900 c         2176 g         1043 t
ORIGIN      
        1 gtccagcagg gtgagcacgg cctggcgccg ggcgcgctgg ctgcgcaggg ggcgcgccag
       61 cagggcgtcg agccgctggg cgtattcgtc ggccagcagc tcgatgcgtt cgtgctcggc
      121 caggtacagg cgcgccggcc agcgtgcgcc tgcgggcagg tggggcagca ggcggtcgtt
      181 ttcgatggcg atgtgtcgcg ccagcgcgcg gcgccagcgc tgcaggcgct ggcgcgcgcg
      241 ctcgaagtcg gcgcccgcca cgtcgagcag gtgggcgtgc aactgggctt ccaggtgctg
      301 gtgttcgcgt tcgaagtgtc ccggggacgg tggcgtgggc atggggcgat catcccacgc
      361 tgtgggggcg ccgtggcccc caccccagcc ctcccccagc gggggagggg gtgtgccggc
      421 gccaggggtt gcccgctccc tcgccccttt ggggagaggg ttggggtgag gggctggcgg
      481 ggggccgggt ggcgctgccg gccccacccc aggcctcccc cagcggagga ggggtgtgcc
      541 ggcgccaggg ggtgcccgct cgctcgcccc tttggggaga gggttggggt gaggggctgg
      601 cggggggccg ggtggcgctg ccggccccac cccaggcctc ccccagcgga ggaggggtgt
      661 gccggcgcca gggggtgccc gctcgctcgc ccctttgggg agagggttgg ggtgaggggc
      721 tggcgggggg ggccgggtgg cgctgccggc gcccagccca ggcctgcccc agcgggggag
      781 gggtgaaggc gcgcctcagg cgtgtgcgag gacgggtgcg gcctgggtac cggcgggcgc
      841 ggtggtgggc ggcagcggct cgaagcgggc ggtgaagaag cgcaggaact tgggttcgta
      901 caccatcttc atgccctgga tctcgcgccg gcgcgcatac aggtccttgc aggcgcgcgc
      961 caccacgtcg aggtgggcgt aggtgtagac gcggcgcggc agcgtcaggc gcaccagctc
     1021 cagcttgggg cggtggtggt cgccggtttc cttgttgcgg ccggccgaga cgatgccgcg
     1081 ctccatgctg cgcacgccgc tttccaggta gagctgggcc gccagcgtct gggccggaaa
     1141 ctcgtcctgc ggcaggtgat ccaggaaggc ccgcgcgtcc aggaacaccg cgtgcccgcc
     1201 caccggcttg acgatgggga tgcccgcggc ctccagctgg tccgccaggt agcggcactg
     1261 gccgatgcgg tggctgatgt agtgctggtc cacggattct tcgatgccgc gcgccatggc
     1321 ctccatgtcg cgcccggcca ggccgccgta gctgggcatg ccctcgaaca gcaccaccag
     1381 ctcgctggcg cgctggtaca gctcgtcgtc gttcatgcac agaaagccgc cgatgttgac
     1441 caggcagtcc ttcttgccgc tcatggtgca gccgtcggcg tggctcatca tctccttgag
     1501 gatggcgcga atgctgacgt cggcgtagcc gggctcgcgc tcctggatga agtaggcgtt
     1561 ctcgacgcag cgcgtggcgt cgaacatcac cttgatgccg tggcgcgcgc acagctcctt
     1621 gacggcgcgc aggttgccca tgctgacggg ctggccgccc gccaggttga cggtgacggc
     1681 cacgcagaca tacggaatgc gctcgggccc gacctcgtcg atcaggcggc tgagcttggc
     1741 gatgtcgacg ttgcccttga agggcagctc gacccgcggg tcgtgcgcct ggtcgttgat
     1801 gatgtcgacg aaggtggcgc cgttcagctc ctgatgcgcc cgcgtggtgg tgaagtacat
     1861 gttgcccggc aagtagtcgc ccggctggat gcacaggcgc gacagcaggt tctcggcgcc
     1921 gcgcccctgg tgcgtgggca cgatgtgctt gaagccgtag tactcgcgca ccacctcctg
     1981 caggtggatg aagttcttgc tgccggcgta ggcctcgtcg cccagcatca tgccggccca
     2041 ctggtagtcg ctcatcgccg tggtgccgct gtcggtcagc aggtcgatgt agcactggtc
     2101 ggagcgcagc aggaaggtgt tgtagccggc ctcggccagg tactgggcgc gctcggcgcg
     2161 cgtggtcatc ttgatcggct cgaccacctt gatcttgaac ggctcggcgg cgtaggtgcg
     2221 ggtcatggcg tgtctcctgt ctggcaaggg cggttgaaca aggcgtgcgt ggcggcgggc
     2281 cgggtgaccg ggcagtagct tgatccgaat caagcttttc tgctttcaaa cgccaaccga
     2341 cgcaacagaa gccgtatttg aggccttgct ggttgatgtt ttctgtcatt gttttcatgt
     2401 ttacgcactt ttcatgcttt ggtgaccgca ccatgcgcag gggtacggca tgccgccggg
     2461 cgccccaagg cccccgaatg actatgaaaa caatagctgc tggcgcacgc cagaaaagcc
     2521 ccagcaccct gaaacgccca tggatgccgc gcccggcacg gccagcggct cagcccgcct
     2581 cgcgcggcac gaagatggcg tagttgccgg tggccgaggc caccagctgc tcgcccgcca
     2641 gcacgcgcga gctcaggtgc gccaccgtgc ggccgcggtt gaccacttcg gtcaggcaca
     2701 ccagccggcc ggcccgcacg gggcggaagt agctgatggc gatgtcgatg gtggcgcaga
     2761 tctgccctgg ctccagcgtg gggtagagcg ccgaccccat gccggtgtcg gccagcgcga
     2821 acaccaccgc gccatgcacc acgccatgcg ggttcaggtg ctgcggcgcc agcgtcaggc
     2881 tgagctggct gtggccggcg cgctgcacct ccaccttcag atcgatcagg tcggcaaagg
     2941 gatggtgcat ggtggacagg ggcagaagtc agacacgatg ccggcgggcg cgagccgccc
     3001 cggcgcgaca catggcagca tagccgaggc accgaacact atgtttttga tagccgctgg
     3061 cgcacaacca gcaagcgctg gagcctcaaa acacctggaa tgatcgtgaa gactggccgc
     3121 acccgccggt gccatggcgc atggcccccg cgccacacag cctagaaacg gcagtgggac
     3181 gtacccgccc gtgccgtgat cggcgtgcca ggcaagacgc gacggcgccg cgggctggtg
     3241 cccaggcccc gcagctgcgg caggatgaac cagatccagt gcgtgcgctt ggcgccggcg
     3301 cgcagctcgg ccagcgccgc ggcgcagcgg tccgcctgcg cctcgatgaa gcggccgagg
     3361 tcggacacgg cggcgccggc aggtcaggcc gcgtagcccg ggttgggcag cacgccgccc
     3421 accagtttgg ggttgttgag ctggcgcggc gccaccttgc caccgcgcgc cttgagccat
     3481 ttttcgacca ggtcccagac catggggttg ccggccttgg cggcgtcttc ggccaccggc
     3541 gcccagccgg ccaccttgta gctcttgtcg gcgtcgatca gctggccctt caggcgcatg
     3601 tcgctcacgc gcttgcccat gggcgccagg ggatcgaagc tgtagctcag gccaccgacg
     3661 cgcaccatgt cgccgccctg ctggtagtag gggtcggggt tgaacaggtt gtcggccacg
     3721 tcttccatca cggtcttgat catctcgccc ttcatctcgg tgacggtggc gtagctgtag
     3781 gtggtggcgg tcatgtccat cacccattcg cgcgtgatgg gctggccggg cagcaggctg
     3841 gtgccccagc ggaagccggg cgagaacgcc aggtcggcgc cctgcacttc catcagcgca
     3901 tcgacgatga gctggtcgcc ggtgccgttg aagttgccgc gccggtacag caggccttcg
     3961 gtgatggcca gttgctcgga cagcttggcc tcgtaggggg cgcgtatctt gctgatgagc
     4021 gcgtccatgt ccttgtcggc cggcagcatg ttggcaaaca ccggcagcag cttgtagcgg
     4081 aagtccgcca ccttgccgcc cttgacgtcg aagtccagca cgccgaggaa cttgccgttg
     4141 ctgccggcgt tggtgacgat ggtctggccg ccggcgttct tgacgatgct ggccacgggc
     4201 atgccgtcat gcgtgtggcc gcccaggatg gcgtcgatgc cgcgcacgcg gctggccatc
     4261 ttcaggtcca cgtccatgcc gttgtggctg acgacgacca ccacctgggc acccttggcg
     4321 cgggcttcgt cgaccacctt ctgcatgttc tcgtcctgga tgccgaattc ccattcggcc
     4381 accatccagc gcgggttggc gatgggggtg taggggaagg cctgaccgac gatggccacc
     4441 ggcacgccgt tcatctggcg gatcacgtag ggtttgaaca ccgggtcgcc gaagtcggcg
     4501 gtcttgatgt tctgcgccac gaagtcgacc ttgccggcaa agtccttctc gacgatttcc
     4561 tgcacgcgct tcatgcccag ggtgaactcg aagtggccgg tcatggcgtc cacgcccagc
     4621 agcttgcagg cgtccaccat gtcctggccg ttggtccaca gcgccgtgcc gctgccctgc
     4681 caggtgtcgc caccatcgac cagcagcgag ccgggacggc tggcgcgcag gcgcttgacc
     4741 agggtggcca ggtgggcaaa gccgcccacc ttgccgtagc gctgggcggc gcggtcgaag
     4801 tccaggtaag tgagcgcgtg cgcactggcg ctgcccgccg gcacgccagc ggccttgagc
     4861 agctgatcgc ccaccaggtg cggcaggcgg cccttcatgt cgccaaagcc caggttgacg
     4921 ctgggctcgc ggaagtagat gggccgcagc tgcgcgtggc agtcggtgat gtgcaggaac
     4981 gacacgttgc cgaacctggg gatgtcgtac agcccttcgg cggcggtctg cgcgctggct
     5041 tcggcaaacc ggcccagccc catgccggcc acgctggcgg cgctgagcac ctgcaggaat
     5101 tcgcgtttgg agaggttcat atatcagcaa ccactgaatt aaaggaagca aaaaagcccg
     5161 gcagcgccgg gctggggccg gtcacttgtt gaccggcgag ttggggtcga gcagcagcgc
     5221 caccaggtcg gccacctgct tttcggtcag gatgcccttg tggccggcgc gcggcatgtt
     5281 ggagcaggcg ttgtaggcct tgctgttcca gatcttgccc caggtgtact tgaccatttc
     5341 ctgcgcctgc gggctgttgg ggtcggtcac gccacgcagc ttgccgtagt tgtacaggct
     5401 ggtgccgatg gtgccgaagg agatttcctt cttgtcgatc tggtggcagt tgtagcagtt
     5461 gccgccgttg ggggtgtcgg ccttgtcggt ccaggtcagg ccgcggccgc tttgcgccag
     5521 tttttcgcct tctttccagt cgcccaggta cttgccgttg gcgggccact tgatggtggc
     5581 catgttgagc gcctcgatgc gcttggcggt ggcgtcgtcg agcggcttgc cggacacgtc
     5641 ggcggcgttg cacacgcgca tggtttcgtc gtcggtgaag gcctggctga ccttgacgat
     5701 gcccttgtcg tggaagtcgc gctggacgat ctgctgcgtc agggtgtcga gctcggccgc
     5761 cgaggggacg ccggcggagc cgccgccggc gcagccggtc agggtcaggg cgccggcgat
     5821 ggacagggcg aggatggatg tgcggtgggt catggtgcgg gcctccggtt cagcgcttga
     5881 tggttggggc ggcggatttg tcgcccttgg cgttgacgcc caggaacacg cccagggcca
     5941 cggtgacgtc gc
//
LOCUS       DAOOOG010000156         5912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_16097_length_5912_cov_29.191224, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000156 DAOOOG010000000
VERSION     DAOOOG010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16097_length_5912_cov_29.191224"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1048
                     /locus_tag="PK242_10655"
     CDS             62..1048
                     /locus_tag="PK242_10655"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOZ94521.1"
                     /translation="MSSPIRIAIAGHGNLGRGVEAAITRNPDMQLVGIYTRRAPSQIT
                     PLTAGVPVHGMDALAGHASGIDVLILCGGSKDDLPEQGPRLAALCNTVDSFDTHARIP
                     EYFAAVDAPARAHGKTALISAGWDPGMFSINRVLGEALLPEGASYTFWGKGVSQGHSD
                     AIRRVPGVAGGVQYTIPVPAAVQQVRAGGQPQLSTRQKHTRECFVVLAEGADAEAVRQ
                     AIVTMPHYFDEYDTTVHFISAEQLRREHGAMPHGGFVIRSGQTSAGVTQVIEYGLTLG
                     SNPEFTSAVLVAYARAVHRLHQQGRHGALTVFDVAPGLLSPLSAAQLRAQSL"
     gene            1205..2026
                     /locus_tag="PK242_10660"
     CDS             1205..2026
                     /locus_tag="PK242_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ94522.1"
                     /translation="MATASSADALVELRDVTFGYGERAVLRQLSLSVPRGKVTALMGA
                     SGGGKTTVLRLIGGQQRAQQGQVRVAGQDVGTMSEQQLYAARRRMGMLFQFGALFTDL
                     SVFDNVAFPLREHTALPEALIRHIVLMKLDAVGLRGARELMPSQISGGMARRVALARA
                     IALDPDLVMYDEPFAGLDPISLGTAAQLIRQLNDALGITSIVVSHDVAETFRIADQVI
                     ILADGGVAACGTPAEVRASSDPLIRQFVGVDLHGPVQFHYPGPTVEQDFGVEAGP"
     gene            2023..2802
                     /gene="mlaE"
                     /locus_tag="PK242_10665"
     CDS             2023..2802
                     /gene="mlaE"
                     /locus_tag="PK242_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HOZ94523.1"
                     /translation="MSWWRPSDIGFAVRRGLDDMGYAARLFWRLLRLLGPTCRRFGLL
                     RDQIHFLGNYSLAIIAVSGLFVGFVLALQGYNILQRYGSAEAVGMMVALSLLRELGPV
                     VTALLFAGRAGTSLTAEIGLMKAGEQFSAMEMMAVDPIQRIIAPRFWAGVIVMPLLTA
                     VFNAVGVLGGWVVSVPMIGVDPGAFWSQMQGGVDVFDDLGNGVVKSVAFGFTVTFVAL
                     LQGYVAKPTAEGVARATTRTVVMASLMVLGLDFLLTALMFS"
     gene            2827..3327
                     /gene="mlaD"
                     /locus_tag="PK242_10670"
     CDS             2827..3327
                     /gene="mlaD"
                     /locus_tag="PK242_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HOZ94524.1"
                     /translation="MGRSKNDIWVGLFVMIGLAALVFLALQSANLLSLSFQRGYRITA
                     SFDNIGGLKPKAAVRSAGVVVGRVAGIGFDDQRFQARIELDMDPRFHFPRDSSLKILT
                     SGLLGEQYIGIEPGADDKNLAPGDNVTATQSAVVLENLISQFLYNSAAGNASSGAGKP
                     AGGATK"
     gene            3393..4172
                     /locus_tag="PK242_10675"
     CDS             3393..4172
                     /locus_tag="PK242_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HOZ94525.1"
                     /translation="MQATPAAGARWRLRGAAGVAALALLSGCASAPGADPRDPWEAYN
                     RSMYRFNDAIDTAVLKPVATVYRDALPQPVRTGVGNFFGNLGDVWSFVNQTLQAKPEA
                     ALHSFWRVVVNTTMGLGGVLDPASEMRLERRREDFGQTLGHWGVPAGPYFVLPFFGPS
                     TVRDTVALPVDIYGHPLSHVNDVSARNALGATQVVNTRAAVLGATELLESAALDPYSF
                     QRDAYLQKRRNDIHDGNPPADQERYDLDEPAPAGGAAAAGR"
     gene            4299..4964
                     /locus_tag="PK242_10680"
     CDS             4299..4964
                     /locus_tag="PK242_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ94526.1"
                     /translation="MPTPLSLVRRRFLRQAGAALVALATGAAMPWARAADEAPDAMIG
                     RLSGEVLERLRNDKALRNGDMARVMAVVDSQVMPNVNFTRMTASATGPGWRRATPEQR
                     QKLQQEFKTLLVHTYAGALRQVSDQTVEVRPLRVAADDAEVVVRTWVKGRGEPVQLDY
                     RLERTPGQGSGWKIYDLNVLGVWLVDNYRPQFAQQINAGGIDALIRSLAERNQANAGS
                     SRG"
     gene            4973..5278
                     /locus_tag="PK242_10685"
     CDS             4973..5278
                     /locus_tag="PK242_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOZ94527.1"
                     /translation="MLVLPSELLHAQAPACLTMLRDAARAEPGPLVLVDATPLVSFDS
                     SALAVLLECRRDCLLDGKQFAVHGLAPHLRELARLYGVSALLAPPQAAPSPALAPAD"
     gene            5357..>5912
                     /locus_tag="PK242_10690"
     CDS             5357..>5912
                     /locus_tag="PK242_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ94528.1"
                     /translation="MPAISFQSVSKVYPATGKPGSTPLQALDDVSFDIEEGEFFGLLG
                     PNGAGKTTLISILAGLARATAGRVLVHGHDVQTEFVSARRHLGIVPQELVFDPFFTVR
                     EALRFQSGYFGLHHNDDWIDELLEGLGLADKARANMRQLSGGMKRRVLVAQALVHKPP
                     VIVLDEPTAGVDVELRQTLWQFVDG"
BASE COUNT          843 a         2053 c         2087 g          929 t
ORIGIN      
        1 ccgtgacccc gggctgcggg cgcagccacg gcgggtgacg gaccccccga aggaacaccc
       61 catgtcttct cccatccgga tcgcgatcgc cggtcacggc aaccttggcc gtggcgtcga
      121 ggccgccatc acccgcaacc ccgacatgca gctggtcggc atctacacgc gccgcgcacc
      181 gagccagatc acgcctctga ccgccggcgt gccggtgcat ggcatggacg cgctggcggg
      241 ccacgccagc ggcatcgacg tgctgatcct gtgcggcggc tccaaggacg acctgcccga
      301 gcaggggccc aggctggccg cgctgtgcaa cacggtggac agcttcgaca cccacgcgcg
      361 catccccgaa tactttgccg ccgtcgatgc gccggcgcgc gcccatggca agacggcgct
      421 gatctcggcc ggctgggatc cgggcatgtt ctcgatcaac cgcgtgctgg gcgaggcgct
      481 gctgcccgaa ggtgccagct acaccttctg gggcaagggg gtcagccagg gccactccga
      541 cgccatccgc cgcgtgcccg gcgtggccgg cggcgtgcag tacacgattc cggtgccggc
      601 ggcggtgcag caggtgcgcg ccggcggcca gccgcagctg agcacgcgcc agaagcacac
      661 gcgcgaatgc ttcgtggtgc tggccgaggg cgccgacgcc gaggcggtgc ggcaggccat
      721 cgtcaccatg ccgcactact tcgacgagta cgacaccacc gtgcacttca tcagcgccga
      781 gcagctgcgg cgcgagcacg gcgccatgcc gcacggcggc ttcgtgatcc gcagcggcca
      841 gaccagcgcc ggcgtcacgc aggtgatcga gtacggcctg acgctgggca gcaaccccga
      901 gttcacctcg gccgtgctgg tggcctatgc gcgcgccgtg caccgcctgc accagcaggg
      961 tcgccacggc gcgctgacgg tgttcgacgt cgcgccgggc ctgctgtcgc cgctcagtgc
     1021 ggcgcagctg cgggcccagt cgctgtgacc gagtaacttc aaaaatgata gctgctggcg
     1081 cttgatggac aagcgctgaa gctggattgt ttgcctgttc ctggggctgc gcccggcctc
     1141 tgccggcggc gcgaccgatc ccgtatcatt ggtcggccgg ccctggggcc ggctttttga
     1201 tggcatggct accgcttcct ccgctgacgc attggtcgaa ctgcgcgacg tgaccttcgg
     1261 gtacggcgaa cgcgcggtgc tgcgccagct gtcgctcagc gtgccgcggg gcaaggtgac
     1321 ggcgctgatg ggggcttcgg gcggtggcaa gaccaccgtg ctgcgcctga tcggcgggca
     1381 gcagcgcgcc cagcagggcc aggtacgggt ggcgggccag gatgtgggca cgatgagcga
     1441 gcagcagctg tacgcggcgc gccggcgcat gggcatgctg tttcagttcg gcgcgctgtt
     1501 caccgacctg tcggtgttcg acaacgtggc ctttcccttg cgcgaacaca ccgcgctgcc
     1561 cgaggcgctg atccgccaca tcgtgctgat gaagctggac gccgtcggcc tgcgcggggc
     1621 gcgcgagctg atgccgtcgc agatttcggg cggcatggcg cggcgcgtgg cgctggcgcg
     1681 cgccatcgcg ctcgatcccg acctggtgat gtacgacgag ccgtttgccg ggctggatcc
     1741 gatctcgctc ggcacggcgg cgcagctgat ccgccagctg aacgatgcgc tgggcatcac
     1801 cagcatcgtg gtgtcgcacg acgtggcgga aacctttcgc atcgccgacc aggtgatcat
     1861 cctggccgac ggcggcgtgg ccgcctgcgg cacgccggcc gaggtgcgcg ccagcagcga
     1921 cccgctgatc cgccagttcg tcggcgtcga tctgcacggg ccggtgcagt ttcactaccc
     1981 tggcccgacg gtggagcagg acttcggggt ggaggcggga ccgtgagctg gtggcgcccg
     2041 tccgacatcg gctttgccgt gcgccgtggc ctggacgaca tgggctatgc cgcgcgcctg
     2101 ttctggcgcc tgctgcggct gctggggccg acctgccggc gcttcggcct gctgcgcgac
     2161 cagatccatt tcctgggcaa ctactcgctg gccatcattg ccgtctccgg cctgttcgtc
     2221 ggcttcgtgc tggcgctgca gggctacaac atcctgcagc gctacggctc ggccgaggcg
     2281 gtgggcatga tggtggcgct gtcgctgctg cgtgaactgg ggccggtggt gacggcgctg
     2341 ctgttcgccg gccgcgctgg cacctcgctg accgccgaga tcggtctgat gaaggcgggc
     2401 gagcagtttt cggccatgga gatgatggcg gtcgacccga tccagcgcat cattgcgccg
     2461 cgcttctggg ccggcgtgat cgtcatgccg ctgctgacgg cggtgttcaa cgccgtcggc
     2521 gtgctgggcg gctgggtggt cagcgtgccc atgatcggcg tcgatcccgg cgccttctgg
     2581 agccagatgc aaggcggtgt cgatgtgttc gacgacctgg gcaacggcgt ggtcaagagc
     2641 gtggcgttcg gcttcaccgt caccttcgtg gcgctgctgc agggctatgt ggccaagcct
     2701 accgccgagg gcgtggcgcg cgccaccacg cgcacggtgg tgatggcgtc gctgatggtg
     2761 ctggggctgg acttcctgct cacggcgctg atgttcagtt gatttgctgc ggggagaaaa
     2821 cgcaagatgg gacgctccaa gaacgacatc tgggtcggcc tgttcgtgat gatcggcctg
     2881 gcggcactgg tgttcctggc gctgcagtcg gccaacctgc tgtcgctgag tttccagcgc
     2941 ggctaccgca tcacggccag tttcgacaac atcggcgggc tcaagcccaa ggccgcggtg
     3001 cgcagcgccg gtgtggtggt cgggcgcgtg gccggcatcg gcttcgacga ccagcgcttt
     3061 caggcgcgca tcgagctgga catggacccg cgctttcact tcccgcggga cagctcgctg
     3121 aagatcctca ccagcggcct gctgggcgag caatacatcg gcatcgagcc cggcgccgac
     3181 gacaagaacc tggcgcccgg cgataatgtg acggccaccc agtccgccgt cgtgctcgag
     3241 aacctgatca gccagttcct ctacaacagc gcggccggca acgccagcag cggcgccggc
     3301 aagccggcgg ggggcgcgac caagtagcgt cacgcggcac ttcggtggcc gccaccatca
     3361 tgcacaccca ccaccaccta cccgcgaccc ccatgcaggc cacgccggcc gccggcgccc
     3421 ggtggcgcct gcgcggcgcc gccggcgtgg cggcgctggc cctgctgagc ggctgcgcca
     3481 gcgcgcccgg ggccgacccg cgcgatccgt gggaggccta caaccgcagc atgtaccgct
     3541 tcaacgacgc gatcgacacc gcggtgctca aacccgtggc cacggtctac cgcgacgcgc
     3601 tgccgcagcc ggtgcgcacc ggcgtgggca acttcttcgg caacctggga gacgtctggt
     3661 cgttcgtcaa ccagacgctg caggccaagc cggaagccgc cctgcattcg ttctggcgcg
     3721 tggtcgtcaa caccaccatg ggcctgggcg gcgtgctcga tccggccagc gagatgcgcc
     3781 tggagcgccg gcgcgaggac ttcggtcaga ccctgggaca ctggggcgtg cccgccgggc
     3841 cgtacttcgt gctgccgttc ttcgggccct cgaccgtgcg cgacacggtg gcgctgccgg
     3901 tggacatcta cggccacccg ctcagccacg tgaacgacgt gtcggcacgc aatgcgctgg
     3961 gcgccaccca ggtggtcaac acccgcgccg cggtgctggg tgccaccgaa ctgctcgagt
     4021 cggccgcgct cgacccctat tccttccagc gcgacgccta cctgcaaaag cgccgcaacg
     4081 acatccatga cggcaatccg ccggccgacc aggagcgcta cgatctggac gaacccgcgc
     4141 ccgccggcgg ggccgcggcg gcaggccgtt agccggcagg gcggcgccgc cccggcagct
     4201 gcggcacaat ttgtaactgc gcccgggaac tcgggttgca ccatgccttc caaagccggt
     4261 cgtcatcagg tccggcccag caccgaacaa taaccaccat gcctaccccc ctgtctttgg
     4321 tccgtcgtcg ttttctgcgc caggctggcg ccgccctggt cgcgctggcc acgggcgcgg
     4381 ccatgccatg ggccagggcg gccgacgaag cgccggacgc catgatcggg cggctgtccg
     4441 gcgaggtgct ggagcgcctg cgcaacgaca aggcgctgcg caacggcgac atggcgcgcg
     4501 tgatggcggt ggtggacagc caggtcatgc ccaacgtcaa cttcacccgc atgaccgcgt
     4561 cggccaccgg gccgggctgg cggcgggcca cgcccgagca gcgccagaag ctgcagcagg
     4621 agttcaagac cctgctggtg cacacctacg ccggcgcgct gcgccaggtc agcgaccaga
     4681 cggtcgaggt gcggccgctg cgcgtcgccg ccgacgacgc ggaggtggtg gtgcgcacct
     4741 gggtcaaggg gcgaggcgag ccggtgcagc tggactatcg gctcgaacgc acgccgggtc
     4801 agggcagcgg ctggaagatc tatgacctga atgtgctggg cgtctggctg gtcgacaact
     4861 accggccgca gttcgcgcag cagatcaacg ccggcggcat cgacgccctg atccgctcgc
     4921 tggccgaacg caaccaggcc aacgccggca gttccagagg ctgagccggg ccatgctggt
     4981 gctgccgtcc gagctgctgc acgcccaggc cccggcctgc ctgaccatgc tgcgcgacgc
     5041 cgcgcgggcc gagcccgggc cgctggtgct ggtcgatgcc acaccgctgg tgagcttcga
     5101 ctcctcggcg ctggcggtgc tgctggaatg ccggcgcgac tgcctgctcg acggcaagca
     5161 gttcgccgtg catggtctgg cgccgcatct gcgcgagctg gcgcgtctgt acggcgtgtc
     5221 ggccctgctg gcgccgcccc aggcggcgcc gtcgccggcc ctggctccgg cggactgaat
     5281 acccccgggt ggcccggatg cttgcgtgcc ggcacccgcc cggcagcggg caagaacgta
     5341 aaatggccgg ttgcctatgc ccgccatttc gtttcagtcg gtgtccaagg tctacccggc
     5401 caccggcaag cctggcagca ctcccctcca agcgctcgac gatgtcagct tcgacatcga
     5461 ggaaggggag ttctttggcc tgctcggccc caacggtgcc ggcaagacca cgctgatctc
     5521 catcctggcc ggcctggcgc gcgccactgc cggtcgcgtg ctggtgcatg gccacgacgt
     5581 gcagaccgag ttcgtgtcgg cgcgccggca tctgggcatc gtgccgcagg agctggtgtt
     5641 cgaccctttc ttcaccgtgc gcgaggcgct gcggtttcag tcggggtact tcggcctgca
     5701 ccacaacgac gactggatcg acgaattgct ggagggcctg ggtctggccg acaaggcccg
     5761 ggccaacatg cgtcagctgt ccggtggcat gaagcgccgc gtgctggtgg cgcaggcgct
     5821 ggtgcacaag ccgccggtga tcgtgctcga cgagccgacc gccggcgtcg acgtggagct
     5881 gcgccagacg ctgtggcagt tcgtcgacgg cc
//
LOCUS       DAOOOG010000157         5901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_16157_length_5901_cov_28.627438, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000157 DAOOOG010000000
VERSION     DAOOOG010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16157_length_5901_cov_28.627438"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(401..1219)
                     /locus_tag="PK242_10695"
     CDS             complement(401..1219)
                     /locus_tag="PK242_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOZ94529.1"
                     /translation="MTPRSTLAALLCAALLAAGCASTTSGSVATGGRGRSQLLLVSSE
                     QVMQQSLQYYDEQNREARAKGQLATSGPEWGRVNAIMQRLVPQVAAFRPDAARWPWQL
                     VLIDEDSVNAHVMAGGKITFYTGLIRKLRLSDDEIAAVMGHEMAHALREHTREKMSQA
                     AAGDLAIAVGGALLGVGQGAVQLASLGKQLALDLPFSRHMESEADLYGLELAARAGYD
                     PRAAVSLWQKMAQAGGGNGTGFLSTHPSAGDRMGQLQAAMPRVLPLYEAARARH"
     gene            complement(1419..1586)
                     /locus_tag="PK242_10700"
     CDS             complement(1419..1586)
                     /locus_tag="PK242_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94530.1"
                     /translation="MQGTTLRRWSFWLLLTGFVAWDFASSAPVNLRLEPPIITAGSGS
                     PASGGHCAMPK"
     gene            complement(1604..2740)
                     /locus_tag="PK242_10705"
     CDS             complement(1604..2740)
                     /locus_tag="PK242_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOZ94531.1"
                     /translation="MNTLAPALDAAAPTRAARWSQRLPLAIAVLALFGWLLWSVSARQ
                     ALLLLVGVGLGAVLAGARFGFTTGWRMLVEQRDPAGVYGQLLLLALLSALSMPLLARF
                     PETTAALGPLSVSLLVGAFVFGLCMQIADGCGSGTLYKAGLGVPLNMAILPLFALGSF
                     AGSAHLNDWLALGALPPVGLVSAFGAGGALAATGVALALIALGVGRWSGQRLDLRRLP
                     RRWLWGALGLALLAALNLLIAGQPWGVVYGFGLWAAKLATALGAFEPSANAFWSEPGH
                     AERLQQTLLLDVTSITNIGILAGALWVSARRPESPRQLTGAQWAIGLLAGFIMGYSSR
                     LAFGCNVGAMVSGISTGSLHGWLWVPLAFAGTLIGLRVRRRFGF"
     gene            complement(2737..3693)
                     /locus_tag="PK242_10710"
     CDS             complement(2737..3693)
                     /locus_tag="PK242_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOZ94532.1"
                     /translation="MRRPLRFLLLALAWATASLAAQPLLTPAQVKALATDPVVRLIDV
                     REASAYAMQHLPGALSAPYGRWRTSDANLGLPPTPAELTQLVQELGLTPDTHAILVYT
                     GIDATDFGGAARAYWTLKSLGMRRLSILNGGLTAWKAEGLPVSDQPARAPRSQWQPRL
                     QAQWLATRDDVRASLAQPEVLRVDARPAPYFQGRLAHDNARARGTLPGAVHLDSEVFF
                     ELGSAALLDKATLESEAQALNATPGQAIITFCNAGHWSATDWFVLSEVLGQPNVRMYP
                     GSMVDWTRASAPLPMIHEPGRWQQLRYALLTWAHRNLGTPAP"
     gene            3956..4831
                     /locus_tag="PK242_10715"
     CDS             3956..4831
                     /locus_tag="PK242_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ94533.1"
                     /translation="MQFVQLVISGVALGCIYGLIALGFVLIYKATETVNFAQGELMML
                     GAFCGLAGSTLLGFPFWVSVLGTLAAMALFGVLTERLVIRPILGQPAFSIVMLTIGIG
                     YVARGAITMIPTIGTETHTLPVPYKDQVWRLGELVLSAEQVVVIGVTAVLCALLYAMF
                     RFTRLGIAMQAASQNQLAAYYMGIPVKRLNGLVWGLAALVAAIAGLLLAPITFVYANM
                     GFIGLKAFPAAVVGGFGSLPGAIVGGLIIGVVESLAGFYLPEGFKDTAAYIVVLVMLM
                     VKPNGLFGEKLRKKV"
     gene            4833..>5901
                     /locus_tag="PK242_10720"
     CDS             4833..>5901
                     /locus_tag="PK242_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ94534.1"
                     /translation="MRFIFKTSYAQDIRLAKHGGHVFWYGLLAVGLLLAPWLLDEYWL
                     AQLTFVLIYAIAGLGLMLLAGFTGQFSLGHAAFLGVGAYTQAILTNAGWPFPLAMLCA
                     AVLAAAVGVVVGLPALRVKGIYLGIATLAFGFIVEEVLARWESLTGGNAGLMAGAPAM
                     FGWTIDSSAGFYAICLITAVLATLGTLNLLRTPTGRAFIAIRDSEISAQSMGIHLARY
                     KTLAFALSAALAGLAGALYAHKLQFLSPDQFNILQSIDLLLLVVIGGLGSVHGAFLGA
                     IFLIAMPQLIALGKDYLPEAIGQAPGLQAVVYGAVLIAFVLFEPMGLYGRWLKIRTWL
                     QLFPFYRQGMFKRQKSFQKSDR"
BASE COUNT          852 a         2033 c         2097 g          919 t
ORIGIN      
        1 ggctgcgccg ctcttcgaga acgctttcgg cgctccaagc ccgacagcct cctagaaact
       61 gcagttgacc gcttgtgacc ttcgggcagg ccgcatcgat gcggggtctt gcgacttcga
      121 tggccttcac ctcttgggtg agcacgctgc gcacccgccc gtaccgcgct cggcgtgcca
      181 tgcggcgttg ctcctgcttg cgggcaccag cccgcggcgg cgtcgcgtct tgcctggcac
      241 gccgatcacg gcacgggcgg gcgcgtccaa ctgccgtttc taggctgcgc ctgcacgcgg
      301 ccgcggaccg tctgccatcg tcgggcggtc ggcatcccgt cagggccgcg cggcctggct
      361 gaccggcacc tgccggggga tgggcgccgt cgctggagac tcagtgccgg gcgcgcgccg
      421 cttcgtacag cggcagcacg cgcggcatgg cggcctgcaa ctggcccatg cggtcgcccg
      481 cgctggggtg ggtggacaga aagcccgtcc cgttgccgcc gccggcctgc gccatcttct
      541 gccacaggct gacggcggcg cgcgggtcgt agccggcgcg cgcggccagc tccagcccgt
      601 acaggtcggc ctcgctttcc atgtggcgcg aaaacggcag gtccagcgcc agctgcttgc
      661 ccaggctggc cagctgcacc gcgccctggc ccacgcccag cagcgcgccg ccgacggcga
      721 tggccagatc gcccgccgcc gcctgcgaca tcttctcgcg cgtgtgctcg cgcagcgcat
      781 gcgccatctc gtggcccatg acggcggcga tctcgtcgtc gctcaggcgc agcttgcgga
      841 tcaggccggt gtagaacgtg atcttgccgc ccgccatgac gtgggcgttg acgctgtcct
      901 cgtcgatcag caccagctgc cagggccagc gcgcggcgtc ggggcgaaac gccgccacct
      961 gcggcaccag gcgctgcatg atggcgttga cgcggcccca ttccggcccg ctggtcgcca
     1021 gctggccttt cgcgcgcgcc tcgcggttct gctcgtcgta gtactgcagc gactgctgca
     1081 tcacctgctc ggacgacacc agcagcaact gcgagcgacc gcggcctccg gtggcgacgc
     1141 tgccgctggt ggtgctggcg cagccggcgg ccagcagggc ggcacagagc aaggcggcaa
     1201 gggtggaacg cggtgtcatg cagcggtcgg tcggtggtgt ggcgaagcgc gacggcgacc
     1261 attgtgccgc caccaccgtt ctctgtgcag ggcgccgcca tggcgcggcg ggcaggttgg
     1321 cggattctgt gacaaatgga ggtctggcgc aggcggggtg tgcgccaaca gctacaaata
     1381 cgatagcgta tgggacgggg cgagtgggcg ccctgacctc acttgggcat ggcgcagtgc
     1441 ccgccgctgg ccgggctgcc ggagccggcg gtgatgatgg gcggctccag ccgcaggttg
     1501 accggcgccg aactggcgaa gtcccaggcc acgaagccgg tcagcagcag ccagaagctc
     1561 cagcggcgca gggtggtgcc ttgcatgtcg atgtcccccg ttctcagaaa ccgaagcggc
     1621 gccgcacgcg caagccgatc agggtgccgg caaaggccag cggcacccac agccagccgt
     1681 gcaggctgcc ggtgctgatg ccgctgacca tggcgcccac gttgcagcca aaggccaggc
     1741 gcgagctgta gcccatgatg aatcccgcca gcaggccgat ggcccactgc gcgccggtca
     1801 gctgccgggg cgactccggg cgccgcgccg acacccacag cgcgccggcc aggatgccga
     1861 tgttggtgat gctggtcacg tccagcagca gggtctgctg cagccgctcg gcgtgacccg
     1921 gctcgctcca gaaggcgttg gccgacggct cgaacgcacc cagcgccgtg gccagcttgg
     1981 ccgcccacag gccgaagccg tacaccacgc cccagggttg gccggcgatc agcaggttca
     2041 gcgccgccag cagcgccagc cccagcgcgc cccacagcca gcggcgcggc agacggcgca
     2101 ggtccaggcg ctggccgctc cagcggccca cgcccagcgc gatcagcgcc agcgccaccc
     2161 cggtggcggc cagcgcgccg ccggcgccga aggcgctcac cagaccgacc ggcggcagcg
     2221 cgcccaacgc cagccagtcg ttcaggtggg ccgaaccggc aaagctgccg agcgcgaaca
     2281 gcggcaggat ggccatgttc agcggcaccc ccagcccggc cttgtacagc gtgcccgagc
     2341 cgcagccgtc ggcgatctgc atgcacaggc cgaacacgaa ggcacccacc agcaggctga
     2401 ccgacagcgg ccccagcgcc gccgtggttt ccggaaagcg cgccagcagc ggcatcgaca
     2461 gcgccgacag cagcgccagc agcagcaact gcccgtatac gccggccgga tcgcgctgtt
     2521 cgaccagcat gcgccagccg gtggtgaagc caaagcgcgc gcccgccagc accgccccca
     2581 ggccgacgcc caccagcagc agcagcgcct ggcgcgccga caccgaccac aacagccagc
     2641 cgaacagcgc cagcacggcg atcgccagcg gcaggcgttg cgaccagcgc gcggcgcggg
     2701 tgggcgccgc ggcgtccagg gcaggggcaa gcgtattcat ggggcagggg tcccgaggtt
     2761 gcggtgcgcc caggtcagca gcgcgtagcg cagttgctgc cagcggccgg gctcatggat
     2821 catgggcaat ggggcggatg cgcgcgtcca gtccaccatc gagccggggt acatgcgcac
     2881 gttgggctgc cccagcactt cggacagcac gaaccagtcg gtggccgacc aatgaccggc
     2941 gttgcagaag gtgatgatgg cctgacccgg cgtggcattc agggcctggg cctcgctctc
     3001 cagcgtcgcc ttgtccagca gcgcggcgct gcccagctcg aagaacacct cgctgtccag
     3061 gtgcacggcg cccggcagcg tgccgcgtgc gcgggcgttg tcgtgcgcca ggcggccctg
     3121 gaaatacggc gccgggcgcg catccacacg cagcacctca ggctgcgcca ggctggcgcg
     3181 cacgtcgtcg cgcgtggcca gccactgcgc ctgcaggcgc ggctgccact ggctgcgcgg
     3241 tgcgcgggcg ggctggtcgc tgacgggcag gccttcggcc ttccaggccg tcagaccacc
     3301 gttgaggatg gacaggcggc gcatgccgag cgacttcagc gtccagtagg cgcgcgcggc
     3361 gccaccgaag tcggtggcgt cgatgccggt gtagaccagg atggcgtggg tgtccggcgt
     3421 caggcccagc tcctgcacca gctgggtgag ttccgctggc gtgggcggca ggcccaggtt
     3481 ggcgtcgctg gtgcgccagc gcccgtaggg ggccgacagc gcgccgggca ggtgctgcat
     3541 ggcgtaggcc gaggcttcgc gcacatcgat cagccgcacc accgggtcgg tcgccagcgc
     3601 cttgacctgt gccggcgtca gcagcggctg ggcagccagc gaggcggtgg cccaggccag
     3661 ggcaagcaac aggaatcgga gcggacggcg catggcgggc acggagcagg gcgatggagc
     3721 aaaccccgca ttgtcgcgcc ggtcgcggtg gcgaccaaat accgagtcgt cagaagcata
     3781 tggggcaccg tcttgcccca catcggtgca cgccggtgtc acggatggga cacggagggt
     3841 gcatggcggc gcgacggtcg cgccacgcta agggtttgtc agggatgtgg ctgcagatca
     3901 agcccgttag agtctggcgc actttgatgc ggcacaagga tagggtggac cgagcgtgca
     3961 atttgtgcaa ctggtgatca gcggggtggc cctgggctgc atctatggtc tgatcgccct
     4021 cggcttcgtg ctgatctaca aggccaccga gaccgtcaac ttcgcccagg gcgagctgat
     4081 gatgctgggc gccttctgcg gcctggccgg cagcaccctg ctgggctttc cgttctgggt
     4141 gtcggtgctg ggcacgctgg cggcgatggc gctgttcggc gtgctgaccg agcggctggt
     4201 gattcgcccc atcctcggtc agccggcgtt ctcgatcgtc atgctgacca tcggcatcgg
     4261 ctatgtggcg cgtggcgcca tcaccatgat cccgaccatc ggcactgaaa cccacaccct
     4321 gcccgtgccc tacaaggacc aggtctggcg gctgggcgag ctggtgctga gcgccgagca
     4381 ggtggtggtg atcggtgtca cggcggtgct gtgcgcgctg ctgtacgcga tgttccgttt
     4441 cacgcgcctg ggcatcgcca tgcaggccgc ctcgcagaac cagctggccg cctactacat
     4501 gggcatcccg gtcaagcgcc tcaacggtct ggtgtggggg ctggcggcgc tggtggcggc
     4561 cattgccggt ctgctgctgg cgccgatcac gttcgtctac gccaacatgg gcttcatcgg
     4621 cctgaaggcg ttcccggccg ccgtggtggg gggcttcggc agcctgccgg gggccatcgt
     4681 cggtggtctg atcatcggtg tggtggagtc gctggcgggc ttttacctgc ccgagggttt
     4741 caaggacacc gccgcctaca tcgtggtgct ggtcatgctg atggtcaagc ccaacggtct
     4801 gttcggcgag aagctgcgca aaaaggtctg acatgcgctt catcttcaag accagctacg
     4861 cgcaggacat ccgtctggcc aagcatggcg ggcatgtgtt ctggtatggc ctgctggcgg
     4921 tcggcctgct gctggcgccc tggctgctgg acgaatactg gctggcgcag ctgaccttcg
     4981 tgctgatcta cgccatcgcc ggtctgggcc tgatgctgct ggccggcttc accgggcagt
     5041 tctcgctggg gcacgcggcg ttcctgggcg tcggcgccta cacccaggcc atcctgacca
     5101 acgccggctg gccgtttccg ctggccatgc tgtgcgccgc cgtgctggcg gccgccgtcg
     5161 gggtggtggt gggcctgccg gcgctgcgcg tcaagggcat ttacctgggc atcgccacgc
     5221 tggccttcgg tttcatcgtc gaggaggtgc tggcgcgctg ggaaagcctg accggaggca
     5281 acgccggcct gatggcgggt gcgccggcga tgttcggctg gaccatcgac tccagcgccg
     5341 gcttctacgc catctgcctg atcaccgccg tgctggccac cctgggcacg ctcaacctgc
     5401 tgcgcacgcc caccgggcgc gccttcatcg ccattcgcga ctcggaaatc tcggcgcaga
     5461 gcatgggcat tcacctggcg cgctacaaga cgctggcctt cgccctgtcg gccgcgctgg
     5521 cgggcctggc gggggcgctg tacgcccaca agctgcaatt cctgtcgccc gatcagttca
     5581 acatcctgca gtcgatcgac ctgctgctgc tggtggtgat cggcggtctg ggctcggtgc
     5641 acggggcgtt tctgggcgcc atcttcctga tcgccatgcc gcagctcatt gcgctcggca
     5701 aggactacct gcccgaagcc atcggtcagg cgcccggcct gcaggcggtg gtctatggcg
     5761 cggtgctgat cgccttcgtg ctgttcgagc cgatggggct gtacggtcgc tggctcaaga
     5821 tccgaacctg gctgcagctg tttcccttct accgccaggg catgttcaag cggcagaaat
     5881 cgttccagaa atcggatcgc c
//
LOCUS       DAOOOG010000158         5863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_16315_length_5863_cov_26.895489, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000158 DAOOOG010000000
VERSION     DAOOOG010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16315_length_5863_cov_26.895489"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..795)
                     /gene="rpe"
                     /locus_tag="PK242_10725"
     CDS             complement(115..795)
                     /gene="rpe"
                     /locus_tag="PK242_10725"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOZ94535.1"
                     /translation="MSTTYRIAPSILSADFARLGQEVRDVIAAGADWIHFDVMDNHYV
                     PNLTFGPMVCQAIKPHAVTPDGRPVPLDVHMMVQPVDALAQAFAQAGADLISFHPDAS
                     AHAHRSVQAIKAAGCQAGLVFNPAAPLDVLDWLIDDIDLILIMSVNPGFGGQSFIDSA
                     LRKVEAARRRIEASGRDIRLEVDGGIKASNIRQVADAGADTFVAGSAIFGQPDYRAVI
                     DAMRRAMA"
     gene            829..1224
                     /gene="apaG"
                     /locus_tag="PK242_10730"
     CDS             829..1224
                     /gene="apaG"
                     /locus_tag="PK242_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HOZ94536.1"
                     /translation="MPTYQFHVEVQPEYLPEQSEPAHSRYGFAYTITVLNTGQVAAQL
                     VSRHWIIVDGRGHTEEVRGLGVVGQQPLLQPGEAFQYTSGCQLRTPSGTMHGSYFCVA
                     EDGERFEVAIPLFVLDASGADGGSARVLH"
     gene            1276..3309
                     /locus_tag="PK242_10735"
     CDS             1276..3309
                     /locus_tag="PK242_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HOZ94537.1"
                     /translation="MAPPRPDLPELLHALDPLAPRAERHLWLMQLLDWVRGQQDEPQA
                     AVARVRMLLDVAEQRPDWLGLWRHWWATFLTEVDATPLLADLGFAARSAFLAELSDRL
                     RRQLIPPTPETTDLRELFALLLPGEFDARWLRALDKPTLERLRRTLFDDAPAEGAPPY
                     ALLTLLNALAYAISQISAAGQSAELRIRMAPEVQRQQPFAALPIGFEALRQAVLSHGR
                     DSAPALVALAQLREQLDACRAAAASVYAHLEEHGISVHIEFQLRQLRQRIIRVKALLL
                     CLETDEPAQATAQLLSHLVRVQLDSRSVGALVRSSTELLAARVTERNAETGEHYIARD
                     RREYLHMLGAAAGGGAVMALTTLSKLVIVGLGLSAFWGGVAAGLNYAISFVIIMLLHW
                     TVATKQPAMTAPTLAARLKALDTPAAHEAFVTEVVHLLRTQFVAIVGNVVVVLALSFA
                     LVAAIGALGGGPWIDDAHAHHILDEHHMLGRTIPFAIVTGGLLFASSLIAGWVENWFV
                     LHRLDSVIAHHPRSTRLLGRERARRWGRYWGSHISGWAANVSLGLLLGLVPPIVGFFG
                     LPVEVRHVTLVSGQIAVAAQQLGPAVWQDPALWWAAGGIAVTGLLDVGVSFYLAFRLA
                     LTAQNVAGVDRRRIYQAMGRRLWRDPWSFVLPVRERPPATQAPADPHKPGQTG"
     gene            3417..4685
                     /locus_tag="PK242_10740"
     CDS             3417..4685
                     /locus_tag="PK242_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOZ94538.1"
                     /translation="MTEPWIAHWTSGSELSAGLITLKWALLLAAAALAGHLSQRLLGL
                     PKLVGYAVFGTLAGLTGLAGADWPLQGAGLTLVELGVAMLLFEAGARLSLRWLRHNPM
                     VLWQSVVESLLTYAAAYLLLRALGLELPVVRALSVVAVASSPAVLLRVVSDLRASGPV
                     SDRAIALATLNTLYALTLGTAMLRSIDRGDVSLLASLGASATVLGISLLTGAALASLL
                     RLAFRVLHPASQDTAIVILALLAACVAVATPLGGSAPLAALLAGLVLKRLYPRPWVWP
                     RQLGTAASMLSMAMFVLVSTLAAQASWSAASALAALGLTGARLLAKLASLLLTGPVSA
                     LSLRQSAWVGVALFPTSAVALLLTSQFVAAAPTVGTPVAAIALPVILLTELLGAALVG
                     LALVRTREAPAPRRWREPAPADTTPPETRP"
     gene            4682..5806
                     /locus_tag="PK242_10745"
     CDS             4682..5806
                     /locus_tag="PK242_10745"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HOZ94539.1"
                     /translation="MTLEAFHTSEALTLGVELELQLVNTNDYDLTHYADNMLRLMARR
                     DLPGSVVPELTTGMIEVSTGICHDWHEARAQLQQIRDALVHSADKLGIAVVGGGTHPF
                     QQWHQQRIYDKPRFHELSQLYGYLSKQFTIFGQHVHVGCPDADSALYTLHCLSRYIPH
                     FIALAASSPFVQGQDTAFDSARLNSVFAFPLSGRAPCVLSWAEFGQYFDKMTRTGVVR
                     SMKDFYWDIRPKPEYGTIEVRVFDTPLTVERAAALAALVQSLAAWIQRERPFQPSEDD
                     YLVYSYNRFQACRFGLEAVYVEPASGRHLPLREHLLATFEQLAGHAADCAASDALALL
                     RADTEAGRNDARWLRERQAREQLLAEVIRQAALRFRGGQP"
BASE COUNT          756 a         2134 c         2085 g          888 t
ORIGIN      
        1 ccttcgcgct gacgcgctgc gggattcgcg cttgggagcg gcccggcgcg ctcatagggt
       61 gtcccttcgc gctgacgcgc tgcgggatgc gcgcttggga gcggcccggc gcgctcatgc
      121 catcgcgcgc cgcatggcgt cgatgacggc gcggtagtcc ggctgaccga agatggcgct
      181 gccggccacg aaggtgtcgg cgccggcgtc ggccacctgg cggatgttgc tcgccttgat
      241 gccgccgtcc acctccagcc ggatgtcgcg gcccgaggcc tcaatgcggc ggcgcgccgc
      301 ttccaccttg cgcaaggcgc tatcaataaa ggactgaccg ccaaagccgg ggttgacgct
      361 catgatcagg atcagatcga tgtcgtcgat cagccagtcc agcacatcca gcggcgccgc
      421 cgggttgaac accagaccag cctggcagcc cgccgccttg atcgcctgca cgctgcggtg
      481 ggcgtgtgcg ctggcgtcgg ggtgaaagct gatcagatcg gcgccggcct gggcaaaggc
      541 ctgggccagc gcgtccaccg gctgcaccat catgtgcacg tccagcggca ccggccggcc
      601 gtcgggcgtg acggcgtgcg gcttgatggc ctggcagacc atcggcccga aggtcaggtt
      661 gggcacgtaa tggttgtcca tcacgtcgaa gtggatccag tcggcgccgg cggcgatcac
      721 gtcgcgcacc tcctgaccca gccgtgcgaa gtcggccgaa aggatggagg gcgcaatgcg
      781 gtaggtggtg ctcatggcgc cggattgtcg cgcaggtacc atgcgcgcat gccgacctac
      841 cagttccacg tcgaggtgca gcccgaatac ctgcccgagc agtccgagcc ggcgcacagt
      901 cgctacggct ttgcctacac catcaccgtg ctcaacaccg gccaggtggc ggcgcaattg
      961 gtgtcgcgcc actggatcat cgtcgacggg cgcggccaca ccgaggaggt caggggcctg
     1021 ggcgtggtcg gccagcagcc gctgctgcag ccgggcgaag ccttccagta caccagcggc
     1081 tgccagttgc gcacgcccag tggcaccatg cacggcagct atttctgcgt cgccgaggac
     1141 ggcgagcgct tcgaggtggc gatccccctg ttcgtgctgg acgccagcgg cgccgacggc
     1201 ggcagcgcgc gcgtgctgca ctgagtgcgt gggcacgctc cccacatgcc caccctctga
     1261 acctgcgcac acccgatggc accgccccgg cccgacctgc ccgaactgct gcacgcgctc
     1321 gacccgctgg cgccgcgcgc cgaacgccat ctgtggctga tgcagctgct cgactgggtg
     1381 cgcggccagc aggatgagcc gcaggccgcg gtggcgcggg tgcgcatgtt gctggacgtt
     1441 gccgagcagc gccccgactg gctggggctg tggcgccact ggtgggccac cttcctgacc
     1501 gaggtggacg ccacaccgct gctggccgac ctgggttttg ccgcgcgcag cgcctttctg
     1561 gccgagctgt ccgaccgcct gcggcgccag ctgatcccgc cgacgccgga aaccaccgac
     1621 ctgcgcgagc tgttcgcgct gctgctgccg ggcgagttcg acgcccgctg gctgcgcgcg
     1681 ctggacaagc cgacgctgga gcggctgcgc cgcacgctgt tcgacgacgc cccggccgaa
     1741 ggcgccccgc cctacgccct gctcacgctg ctgaacgcgc tggcctacgc catcagccag
     1801 atcagcgccg ccggccaatc ggccgagctg cgcatccgca tggcgcccga ggtgcagcgc
     1861 cagcagccgt tcgccgccct gccgatcggc ttcgaggcgc tgcgccaggc ggtgctgagc
     1921 cacggccgcg acagcgcacc ggcgctggtg gcgctggcgc agttgcgcga gcagctcgac
     1981 gcctgccgcg ccgccgccgc cagcgtgtat gcgcacctgg aagagcacgg catctcggtg
     2041 cacatcgaat tccagctgcg ccagctgcgc cagcgcatca tccgcgtcaa ggcgctgctg
     2101 ctgtgcctgg agaccgacga gccggcgcag gccacggccc agctgctgtc gcacctggtg
     2161 cgcgtgcagc tggacagccg cagcgtgggc gcgctggtgc gcagcagcac cgagctgctg
     2221 gccgcgcggg tgaccgagcg caacgccgag accggcgaac actacatcgc ccgcgaccgg
     2281 cgcgaatacc tgcacatgct gggcgcagcg gcgggcggcg gcgccgtgat ggcgctgacc
     2341 acgctgagca agctggtgat cgtcggcctg ggcctgtcgg ccttctgggg cggggtggcg
     2401 gcggggctga actacgccat ctccttcgtc atcatcatgc tgctgcactg gacggtggcc
     2461 accaagcagc cggccatgac ggcgcccacc ctggcggcgc ggctgaaggc gctcgacaca
     2521 cccgccgcgc acgaagcatt cgtcaccgaa gtggtgcatc tgctgcgcac gcagttcgtg
     2581 gccatcgtcg gcaacgtggt ggtggtgctg gcgctcagct ttgcgctggt ggcggcgatc
     2641 ggcgcgctgg gcggcggccc ctggatcgac gacgcgcacg cgcaccacat cctggacgag
     2701 caccacatgc tggggcgcac catcccgttc gccatcgtca ccggtggcct gctgttcgcc
     2761 tccagcctga tcgccggctg ggtggaaaac tggttcgtgc tgcaccggct ggattcggtg
     2821 atcgcgcacc acccgcgcag cacccggctg ctgggccgcg agcgcgcccg gcgctggggc
     2881 cgctactggg gcagccacat ctccggctgg gcggccaacg tctcgctcgg tctgctgctg
     2941 gggctggtgc cgccgatcgt cggcttcttc ggcctgccgg tggaagtgcg ccacgtgacc
     3001 ctggtgtcgg gccagatcgc cgtggcggcg cagcagctgg ggcccgccgt gtggcaggac
     3061 ccggccctgt ggtgggccgc cggcggcatc gccgtcaccg gcctgctgga cgtcggcgtc
     3121 agcttctacc tggcgttccg gctggcgctg acggcgcaga acgtcgccgg cgtcgatcgc
     3181 cgccgcatct accaggccat gggccggcgc ctgtggcgcg atccgtggtc ctttgtgctg
     3241 ccggtgcgcg agcggccgcc ggccacccag gcccctgccg acccccacaa gcccggccaa
     3301 acgggttagc atgggcgctg ccgcgccggc cctggtccgc gcggcctgcc ggggccgccc
     3361 ggggcgccac cagcgcgcgc agcggccgcc ggcaggcatc ggagacgctg actgttttga
     3421 ctgaaccatg gatcgcccac tggaccagcg ggtctgaact ctctgccggc ctgatcaccc
     3481 tcaagtgggc gctgctgctg gccgcggcgg cgctggccgg gcacctgtcg caacgcctgc
     3541 tgggcctgcc caagctggtc ggctatgcgg tgttcggcac cctggccgga ctgaccggac
     3601 tggccggcgc cgactggccg ctgcagggcg ccggcctgac cctggtggag ctgggcgtcg
     3661 ccatgctgct gttcgaagcc ggcgcccgcc tgtcgctgcg ctggctgcgc cacaacccga
     3721 tggtgctgtg gcaaagcgtg gtcgagtcgc tgctgaccta cgccgccgcc tacctgctgc
     3781 tgcgcgcgct ggggctggag ctgccggtgg tgcgggcgct gtcggtggtg gcggtggcgt
     3841 cgtcgccggc ggtgctgctg cgcgtggtgt cggatctgcg cgccagcggg ccggtgagcg
     3901 accgcgccat cgcgctggcc acgctgaaca ccctgtatgc gctcaccctc ggcaccgcca
     3961 tgctgcgcag catcgaccgc ggcgacgtgt cgctgctggc gtcgctgggc gcctcggcga
     4021 cggtgctggg catttcgctg ctgaccggcg ccgcgctggc gtcgctgctg cggctggcgt
     4081 tccgcgtgct gcacccggcc agccaggaca ccgccatcgt catcctggcc ctgctggcgg
     4141 cctgcgtcgc cgtcgccacg ccgctgggcg gctcggcgcc gctggcggcc ctgctggccg
     4201 ggctggtgct caagcggctg taccccaggc cctgggtgtg gccgcgccag ctcggcaccg
     4261 ccgcatcgat gctgagcatg gccatgtttg tgctggtgtc cacgctggcg gcgcaggcca
     4321 gctggagcgc cgcctcggcg ctggcggcgc tgggcctgac cggcgcacgc ctgctggcca
     4381 agctggccag cctgctgctg accggcccgg tcagcgcgct gtcgctgcgc cagtcggcct
     4441 gggtcggggt ggcgctgttt ccgacgtcgg cggtggcgct gctgctgacc tcgcagttcg
     4501 tcgccgccgc ccccaccgtg ggcacgccgg tggcggccat cgcgctgccg gtgatcctgc
     4561 tgaccgagct gctgggggcg gcgctggtgg gcctggcgct ggtgcgcacg cgcgaggcgc
     4621 cggcgccccg gcgctggcgc gagccggcac cggccgacac cacccccccg gagacacgac
     4681 catgacgctg gaggccttcc acacctccga ggcgctgacg ctgggcgtcg agctggagct
     4741 gcagctggtc aacaccaacg actacgacct gacccactac gccgacaaca tgctgcgcct
     4801 gatggcgcgg cgcgacctgc cgggctcggt ggtgcccgag ctgaccaccg gcatgatcga
     4861 ggtgtcgacc ggcatctgcc acgactggca cgaggcgcgc gcccagctgc agcagatccg
     4921 cgacgcgctg gtgcacagcg ccgacaagct gggcatcgcc gtggtgggcg gcggcacgca
     4981 cccgttccag caatggcacc agcagcgcat ctacgacaag ccgcgctttc acgagctgtc
     5041 gcagctgtat ggctacctga gcaagcagtt caccatcttc ggccagcatg tgcacgtggg
     5101 ctgccccgac gccgacagcg cgctctacac cctgcactgc ctgtcgcgct acatcccgca
     5161 cttcatcgcg ctggcggcgt ccagcccgtt cgtgcagggt caggacacgg ccttcgattc
     5221 ggcgcggctg aactcggtgt ttgcctttcc gctgtcgggg cgcgcgccct gcgtgctgag
     5281 ctgggccgaa ttcgggcagt acttcgacaa gatgacccgc accggcgtgg tcagaagcat
     5341 gaaggacttc tactgggaca tccgccccaa gcccgaatac ggcaccatcg aggtgcgggt
     5401 gttcgacacc ccgctgacgg tggagcgcgc cgccgccctg gccgcgctgg tgcagagcct
     5461 ggcggcctgg atccagcgcg agcgcccgtt ccagcccagc gaagacgact acctggtcta
     5521 cagctacaac cgcttccagg cctgccgctt cgggctggag gcggtctacg tcgagccggc
     5581 cagcggccgg catctgccgc tgcgcgagca cctgctggcc acgttcgagc aactggccgg
     5641 gcatgccgcc gactgcgccg ccagcgacgc cctggccctg ctgcgcgccg acaccgaggc
     5701 cggccgcaac gatgcccgct ggctgcgcga gaggcaggcg cgcgaacagt tgctggccga
     5761 ggtgatccgc caggcggcgc tgcgctttcg cggcggtcaa ccctgaggcc gctggcgcgt
     5821 tggccctaca gtcgccccat cgtcacgcac gcgcccgccc cat
//
LOCUS       DAOOOG010000159         5835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_16420_length_5835_cov_26.655190, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000159 DAOOOG010000000
VERSION     DAOOOG010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16420_length_5835_cov_26.655190"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PK242_10750"
     CDS             complement(<1..313)
                     /locus_tag="PK242_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ94540.1"
                     /translation="MRAKNKSIIVTGAGGGIGEGIARRLAVEGAVVIVNDIQPTLGEK
                     VTADIVAAGGQARYVAADVTRGDDWARLVQAAQDFGGRLDVVVNNAGWTHRNRPMLEV
                     SE"
     gene            424..1122
                     /locus_tag="PK242_10755"
     CDS             424..1122
                     /locus_tag="PK242_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HOZ94541.1"
                     /translation="MNGSLIDDLMGQLQGPSLGPIAQQLGTDADTAGQAIAAALPMLV
                     GALGRNAQQPGGAGALFEALQRDHAGSGAMDLGGLLGGLLGGAGAGAGSAGMGGMGGM
                     GDLLGSVLGGGAPASRQLDAGGILGHILGGAQPRAEAGLSQATGLDMGQAGKLLMMLA
                     PIVMAALARRVNAGQLDAGGLGDVLGQERAQLQQQGGLGGGLMNAVLDQNGDGQLDLG
                     DLLKLGGSLLGGRR"
     gene            complement(1285..2718)
                     /locus_tag="PK242_10760"
     CDS             complement(1285..2718)
                     /locus_tag="PK242_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOZ94542.1"
                     /translation="MQYNFIDNASVPSLSGHTLPVLDPSDGQPFDELQRSDARDIDRA
                     VRSARACYDERWSRLSAAERGRLLMALSRKVADHADELALLEQRDCGKPTRQARADAA
                     ALARYFEFYAGACDKLHGETLPYQSGYSVFTWREPHGVTGHVIPWNYPMQIFGRSVGG
                     ALAAGNCCVVKPAEDACLSLLRVAQLAAEVGFPPGTINLVTGYGHEVGDALARHPGID
                     HISFTGSPRVGTLIQQVAAERHCPVTLELGGKSPQIIFADADLDAALPVVINAIVQNA
                     GQTCSAGSRVLIQQSIYDQMLDRLGAAFEALRVGPASMDLDVGPLIRQSQQQRVWDFL
                     SDAQNDGIATVAQGQIVDEAPEAGFYQAPVLLADVPVAHRVAQEEVFGPVLSAMPFRD
                     EDHAVELANATRFGLVAGVWTRDGARQFRMARRVRSGQVFVNNYGAGGGVELPFGGYK
                     SSGYGREKGFEALYGFTVLKTVAVQHG"
     gene            2789..4006
                     /locus_tag="PK242_10765"
     CDS             2789..4006
                     /locus_tag="PK242_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ94543.1"
                     /translation="MTIDPTSRRLPLGIWVLGFVSLLMDVSSEMIHSLLPMFMVGSLG
                     MSVALVGLLEGLAEATALIAKVFSGVLSDWLGRRKRLALLGYGLSAVTKPLFALAAGP
                     AGPALVITARLMDRVGKGIRGAPRDALVADLAPPELRGAAFGLRQALDTVGAFVGPLL
                     ATVLMLLWSDHFRAVFALALVPAALCVLLLALGVREPERPAGRPRINPLSRANLVRLP
                     RAYWSVVGVGAVFTLARFSEAFLVLRAEQTGVAIALVPLVMVAMNAVYALAAYPFGKL
                     ADRLPHARLLFAGLAVLWLADGVLAVATHWSALLLGILLWGLHLAMTQGLLAAMVAAT
                     VPDDLRGTGFGLFYLVSGVAMLAASVVAGLLWQVLGPAWTFGAGAGFCALTMLVLARP
                     AARHKKSPPGGGL"
     gene            complement(4025..>5835)
                     /locus_tag="PK242_10770"
     CDS             complement(4025..>5835)
                     /locus_tag="PK242_10770"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOZ94544.1"
                     /translation="VGDCAGMAADLFETYAVTLIATMVLGALMVTAAPTQAVLYPLAL
                     GGVSIIASIIGTFFVKASPGMKNVMPALYKGLAIAGVLSLIAFYFVTSWIIPDNALGG
                     SGAVMKLFGACVVGLVLTAALVWITEYYTGTQYAPVKHVAQASTTGHATNIIAGIGVS
                     MKSTAWPVIFVCIAIVAAYALAGLYGVAVAAMSMLSMAGIIVALDAYGPITDNAGGIA
                     EMAELPSSVRDITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHKLDTFGQSIT
                     FDLSDPMVIVGLFIGGLIPYLFGAMAMEAVGRAAGSVVEEVRRQFREIKGIMEGTGKP
                     EYGKAVDMLTGAAIKEMMIPSLLPVAVPILVGLVLGPKALGGLLMGTIVTGLFVAISM
                     CTGGGAWDNAKKYIEDGHHGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIV
                     ALLIVPLVVGMHGGKADGAAPTAPAAQVSAAAPAPAVAPASASDDAASVVVEGEKVLF
                     YFATGKADPHPDGSQALATIVNGVKAGKKAAISGYVDSTGSAAANAEVAKQRALAVAA
                     LLKSLGVTDEQMQLQKPEDIQAGSGPQARRVEVTLQ"
BASE COUNT          770 a         1954 c         2137 g          974 t
ORIGIN      
        1 cctcgctcac ttccagcatg gggcggttgc ggtgcgtcca gccggcgttg ttgaccacca
       61 cgtccaggcg cccgccgaag tcctgcgcgg cctgcaccag acgcgcccag tcgtcgccgc
      121 gcgtgacgtc ggcggcgacg tagcgcgcct gcccaccggc cgccacgatg tcggcggtga
      181 ctttttcgcc cagggtcggc tggatgtcgt tgacgatcac caccgccccc tcgaccgcca
      241 gccggcgggc aatgccctcg ccgatgccgc cgccggcgcc ggtgacgatg atggatttgt
      301 tttttgcccg catgtgcttt gctccttgtg acgcagttgg cagttcggac cggatgttac
      361 gcggccgctg ccgtgacgtc gttacgatgt caggtcttca ccgatgcacc ggagggttcg
      421 agcatgaacg gatcactgat tgacgacctg atgggacaac tgcagggccc ctcgctgggc
      481 ccgatcgcgc agcagctggg caccgacgcc gacaccgccg gccaggccat tgccgccgca
      541 ctgcccatgc tggtgggcgc gctggggcgc aacgcgcagc aacctggcgg cgccggtgcc
      601 ctgttcgagg cgctgcaacg cgaccacgcg ggcagcggcg cgatggatct cggcgggctg
      661 ctcggtggcc tgctgggcgg cgccggcgcg ggcgccggca gcgcaggcat gggtggcatg
      721 ggtggcatgg gtgatctgct gggcagcgtg ctgggcggcg gcgcgcccgc cagccggcag
      781 ctcgatgccg gtggcatttt gggtcacatc ctgggcggcg cgcagccacg cgccgaggcg
      841 ggcctgagtc aggccaccgg tctggacatg ggccaggccg gcaagctgct gatgatgctg
      901 gcgcccatcg tgatggccgc gctggccagg cgagtcaacg ctggccagct cgatgccggc
      961 ggactgggcg atgtgctggg gcaggagcgt gcccagctgc agcagcaggg cggcctgggc
     1021 ggcggcctga tgaacgccgt gctggatcag aacggcgatg gccaactcga tctgggcgac
     1081 ctgctcaagc ttggcggcag cctgctcggc ggtcggcgct gaaggactgg cctgttgcgc
     1141 ttcgggcggc ggcgcgacag gatatgcccc gcacgcagag gacgcagcga tggcgccgaa
     1201 gacgctgaaa gatgcggttg tcttttgcgt gcgctgcgat accgtggtgc ctgctgcgcg
     1261 gggacgcccg ggcgtccgac ctgatcaccc gtgctgtacg gcgaccgtct tgagcaccgt
     1321 gaagccgtac agcgcctcga agcccttttc gcgcccatag ccggacgact tgtagccgcc
     1381 aaagggcagt tccacgccac cgccggcgcc gtagttgttg acgaacacct gaccgctgcg
     1441 aacgcgccgc gccatgcgga actggcgcgc gccgtcgcgc gtccagacac cggccaccag
     1501 gccgaaacgg gtggcgttgg ccagttccac cgcgtggtcc tcgtcgcgaa acggcatcgc
     1561 cgacagcacc gggccgaaca cctcttcctg cgccacgcgg tgcgccaccg gcacgtcggc
     1621 cagcagcacc ggggcctggt agaagccggc ttcgggcgct tcgtcgacga tctggccctg
     1681 cgccaccgtc gcgatgccgt cgttctgggc gtccgaaaga aagtcccata cgcgctgttg
     1741 ctggctctga cggatcaggg ggccgacgtc caggtccatg ctggccgggc ccacgcgcag
     1801 ggcctcgaag gcggcaccca ggcggtcgag catctggtcg taaatgcttt gctggatcag
     1861 cacgcgcgaa ccggccgagc aagtctgacc ggcgttctgc acgatggcgt tgatgaccac
     1921 cggcagggcg gcgtccaggt cggcgtcggc aaagatgatc tgtgggctct tgccgcccag
     1981 ttccagcgtc accgggcagt ggcgctcggc ggccacctgc tggatcagcg tgcccacgcg
     2041 cgggctgccg gtgaagctga tgtggtcgat gccggggtgg cgcgccagtg cgtcgcccac
     2101 ctcgtggccg tagccggtga ccaggttgat ggtgcctggc ggaaagccca cttccgccgc
     2161 cagttgcgcc acccgcagca acgacaggca ggcgtcctcg gccggcttga ccacgcagca
     2221 gttgcccgcc gccagcgcac cgcccacgct gcggccgaag atctgcatcg ggtagttcca
     2281 ggggatcacg tggccggtca cgccgtgcgg ctcgcgccag gtgaagacgc tgtagccgct
     2341 ctggtagggc agggtctcgc cgtgcagctt gtcgcaggcg ccggcgtaga actcgaagta
     2401 gcgcgccagc gcggcggcgt cggcgcgcgc ctgtcgggtg ggtttgccgc agtcgcgctg
     2461 ctccagcagc gccagctcgt cggcatggtc ggcgaccttg cgcgacagcg ccatcagcag
     2521 gcgcccgcgt tcggcggccg acaggcggct ccagcgctcg tcgtagcagg cgcgcgccga
     2581 gcgcacggcg cggtcgatgt cgcgcgcgtc gctgcgctgc agttcgtcga agggctgacc
     2641 gtcggagggg tcgagcaccg gcagggtgtg gcccgacagg gacggcacgg aggcgttgtc
     2701 gatgaagttg tattgcatgg cggtattgtg ccgccgtgcg gcacggatcg cgcggcacca
     2761 tcggcgccgt ccgcgtgggt gcatcaccat gaccatcgat ccaacgtccc ggcgcctgcc
     2821 cttgggcatc tgggtgctgg gcttcgtcag cctgctgatg gatgtctcct ccgagatgat
     2881 ccacagcctg ctgccgatgt tcatggtcgg ctcgctgggc atgagcgtgg ccctggtcgg
     2941 cctgctggaa ggcctggccg aggccacggc gctgattgcc aaggtgttct cgggtgtgct
     3001 gagcgactgg ctgggtcggc gcaagcggct ggcgttgctc ggctacggcc tcagcgcggt
     3061 gaccaagccg ctgtttgccc tggccgccgg gccggcgggg ccggcgctgg tgatcaccgc
     3121 gcgcctgatg gaccgggtcg gcaagggcat tcgcggcgcg ccgcgcgatg cgctggtggc
     3181 tgatctggct ccgcccgaac tgcgcggcgc ggcgttcggc ctgcgtcagg cgctggacac
     3241 cgtgggcgcc ttcgtcggcc cgctgctggc gacggtgctg atgctgctgt ggtccgacca
     3301 cttccgcgcc gtgttcgcgc tggcccttgt gccggcggcg ttgtgtgtgc tgctgctggc
     3361 cctgggcgtg cgcgaacccg agcgccccgc cggcaggccc cgcatcaacc ctctcagccg
     3421 cgccaacctg gtgcgtctgc cgcgcgccta ctggtcggtg gtgggcgtgg gcgcggtgtt
     3481 cacgctggcg cgcttttcgg aggcctttct ggtgctgcgc gccgagcaga ccggcgtggc
     3541 gatcgcgctg gtgccgctgg tgatggtggc catgaacgcg gtgtacgcgc tggcggccta
     3601 cccctttggc aagctggccg accgcctgcc ccacgcccgc ctgctgttcg ccggactcgc
     3661 ggtgctgtgg ctggccgatg gcgtgctggc cgtggccacc cactggtcgg cgctgctgct
     3721 gggcatcctg ctatggggtc tgcacctggc gatgacgcag gggctgctgg cggccatggt
     3781 ggccgccacg gtgcctgacg acttgcgcgg caccggcttt ggcttgttct acctggtcag
     3841 tggcgtggcc atgctggcag ccagcgtcgt ggcgggcctg ctgtggcagg ttctggggcc
     3901 ggcatggacg tttggcgccg gggccgggtt ttgtgcactg acgatgctgg tgctcgcccg
     3961 cccggccgca cggcacaaaa aaagcccgcc gggcggcggg ctttgatgtg gccggtgggc
     4021 gcgcttactg caacgtcacc tcgacgcggc gtgcctgcgg gccgctgccg gcctggatgt
     4081 cttcgggctt ttgcaactgc atctgctcgt cggtcacgcc caggctcttg agcagcgcgg
     4141 ccacggccag ggcgcgctgc ttggcgactt cggcgttggc ggcggcgctg ccggtgctgt
     4201 ccacgtagcc gctgatggcg gccttcttgc cggccttgac gccgttgacg atggtggcca
     4261 gcgcctggct gccgtccggg tgcgggtcgg ccttgccggt ggcgaagtag aacagcactt
     4321 tctcaccttc gaccaccacc gaggcggcat cgtcgctcgc gctggccggg gctacggcag
     4381 gggccggcgc cgcagcgctg acctgggctg ccggcgccgt gggggcggcg ccgtcggcct
     4441 tgccgccatg catgcccacc accagcggca cgatcagcag cgccacgatg ttgatgatct
     4501 tgatcagcgg gttgacggcc gggccggcgg tgtccttgta ggggtcgccc acggtgtcgc
     4561 cggtgacggc ggccttgtgc gcctcgctgc ccttgccgcc gtggtggccg tcctcgatgt
     4621 acttcttggc gttgtcccag gcgccgccgc cggtgcacat ggagatggcg acgaacaggc
     4681 cggtgacgat ggtgcccatc agcaggccgc ccagcgcctt cggccccagc accaggccga
     4741 ccaggatcgg caccgccacc ggcagcaggc tggggatcat catttccttg atggccgcgc
     4801 cggtcagcat gtcgacggcc ttgccgtatt cgggtttgcc ggtgccttcc atgatgccct
     4861 tgatctcgcg gaactggcgg cgcacctctt cgaccacgct gccggcggcg cggccgacgg
     4921 cctccatcgc catggcgccg aacaggtagg ggatcaggcc gccgatgaac aggcccacga
     4981 tcaccatcgg gtcggacaga tcgaacgtga tcgactggcc gaaggtgtcg agcttgtgcg
     5041 tgtagtcggc aaacagcacc agcgcggcca agccggccga gccgatggcg tagcccttgg
     5101 tcaccgcctt ggtggtgttg cccacggcgt ccagcgggtc ggtgatgtcg cgcacgctgc
     5161 tgggcagctc ggccatttcg gcaatgccgc cggcgttgtc ggtgatgggg ccgtaggcgt
     5221 ccagcgcgac gatgatgccg gccatcgaca gcatcgacat ggcggccacc gcgacgccgt
     5281 acaggccggc cagggcatag gccgcaacga tggcgatgca gacgaagatc accggccagg
     5341 cggtggactt catggacacg ccgatgccgg cgatgatgtt ggtggcgtgg ccggtggtgg
     5401 aagcctgcgc cacgtgcttg accggggcgt actgcgtgcc ggtgtagtac tcggtgatcc
     5461 acaccagcgc cgccgtcagc accaggccga ccacgcaggc gccgaacagc ttcatcacgg
     5521 cgccgctgcc gcccagggcg ttgtcgggga tgatccagct ggtcacgaag tagaaggcga
     5581 tcagcgacag cacgccggcg atcgccaggc ccttgtagag cgccggcatc acgttcttca
     5641 tgccgggact ggccttgacg aagaaggtgc cgatgatgga ggcgatgatc gacacgccgc
     5701 ccagcgccag cgggtacagc acggcttgcg tcggcgcggc ggtgaccatc agggcgccca
     5761 gcaccatggt ggcgatcagg gtgacggcgt aggtctcgaa caggtcggcc gccatgccgg
     5821 cgcagtcgcc cacgt
//
LOCUS       DAOOOG010000160         5768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_16696_length_5768_cov_24.525993, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000160 DAOOOG010000000
VERSION     DAOOOG010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16696_length_5768_cov_24.525993"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PK242_10775"
     CDS             <1..924
                     /locus_tag="PK242_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOZ94545.1"
                     /translation="QEATVTEDITGAIADSVNYTVEELRSLVGNVQSTATRVAQTTTQ
                     VEATSTELLATSTEQLREIRETGQSVLDMATRINEVSSQAQQSATVARQSLQAAESGL
                     TAVQNAIGGMNAIRDQIQETSKRIKRLGESSQEIGEITELIADITEQTNVLALNAAIQ
                     AASAGEAGRGFSVVAEEVQRLAERSGDATRQIAALVKAIQTDTQDAVAAMERSTQGVV
                     EGARLSDNAGTALSEIDRVSRQLAELIERISASASKEADQANEVAGNIQHIFAVTEQT
                     GEGTRTTATQVRELARMADELRQSVARFKIA"
     gene            989..>5768
                     /locus_tag="PK242_10780"
     CDS             989..>5768
                     /locus_tag="PK242_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOZ94546.1"
                     /translation="MHDIMVMSPAAEMRDSGASAVRDLGPLAWVLDETRRSIEAASKS
                     LKRFARETDAARGIDLGSVDASPLRMARQQLHQVAGALDMVDQSVAALMVRGMEGAVQ
                     QFVQRPDKCTEETAQALERAGFALMEYLEAQMGSHPRSAVGLFPQYRRVQELAGADRI
                     HPADLWSVPWSWADPVVPASAQPLSYDPEVRHRLDQRVLKILRHPAAHAADELAVISL
                     SLAAGQMQRQATVFWLLSAGFFEALGRGLIVKDVYAKRAASRILMQYTSLARGGQVVS
                     ERLAHDLLFFCSQARFDADANTPALQAVRKAWSLDAQQATSYEQAMFGLYDPAILAQA
                     RRRIESVKENWSALTGGDLARMKACVDQFSLVAESLDKLHEQGSVLGEVLNRVAQQTA
                     QSGRPPGPELAMETATAVLFLEASFADFHPGDAMFSTRLAQLAARLDAVRAGASAPPL
                     EPWMEELYRNVSDRQTMGTVVGELRTTLGEVEHLLDQFFRQPADRSTLVSVPGLMGQM
                     RGVLSVLGLDQAALAVGRMRDLVDELLAAQPDTKLADSAPSFEKLGDTLGALGFQIDM
                     LSYQPVLARKLFVYDADAGELRYRSGRAATQVTTEQNSVASATTDIDLLLDAGTELTV
                     PTSGPGSLVDESLPSETISGQLDEIATMAALAEQRALADAAQRAADAARRHDEAGLSG
                     ALDALQALTAASTPQPTSTTVEGGGADDDDLQAIFLEEAQQVMDTGCEVVQALLTAPG
                     DMELQTALRRAFHTLKGSSRMVGLTEFGEAAWAMEQLFNAWLPEQKPVTEDMRGLALE
                     ALQGLHAWTEAIAGRNDHGWTSAPFRQAADALRLSGQRVSLSCWPDAQVVVDAGAGHA
                     DVVGDGAAPAEQPHEVSSESIPAVQPAPPGERGLPDPQVPELAEGVVEVDATPADVAS
                     ELEQQPAIELTELNLDAGDDPDAGSGLEVIDLSVSRWGPISETPKPQGLASGEPTQPP
                     VELELPSAVDVQMPVAFWPATETVESIPPVGGMVGDEAALPEATLVSPEGLSAPQEGA
                     EDGQTADVAAQDAELYRQIGDLRVSTALYNVYLNEADEWSRQLATDLSEWALELDRPV
                     PDAAVARAHSLAGSSATVGFAALSELARAVEHALQRLVGQSRGTTEQAQCLNEAAQDI
                     RRVLHQFAAGMLKTPNPDILAAVQALEPVVAGDEVAPALAQASASTWMDLAETVPLSS
                     TKDEAQPPVVTRDSLQSLRALGDDIDVADVIDPDLFPIFEEEAEELLPQMGTALRRWV
                     AHPDDREARAQALRALHTLKGSARLAGALRLGELAHRTESAVETLGLDQLVSADLEPL
                     LDHFDELQAGFRRLQAGEQGVETSQVASVPDVATAEDARPAEEPSAEAGQGESRVVVP
                     TVTRGDIASPAALSLVSARAAASQAVRVRSQLLDRLVMQAGEVATSRARLEAEIGQLR
                     GLLGELTGNLDRLRGQLRDVELQAETQMQSRLALSRDAQQNFDPLEFDRFTRMQELTR
                     MMAESVNDVATVQRSLQRAVEGSEDNLAAQARQSRELQRELLRTRMVEFDSIAERLYR
                     VVRQASKETGKQVRLDIVGGATEIDRGILERMTPAFEHLVR"
BASE COUNT          964 a         1810 c         2068 g          926 t
ORIGIN      
        1 caggaagcca cggtgaccga ggacatcacc ggcgccatcg ccgactcggt gaactacacg
       61 gtggaggaac tgcgctcgct ggtgggcaac gtgcagagta cggccacccg cgtggcgcag
      121 accacgaccc aggtggaagc cacgtcgacc gagctgctgg cgacttctac cgagcagctg
      181 cgcgagatcc gcgagaccgg ccagtcggtg ctcgacatgg ccacgcgcat caacgaggtg
      241 tcatcgcagg cccagcagtc ggccacggtg gcgcgccagt cgctgcaggc agcggaatcc
      301 gggttgaccg ccgtgcagaa cgccattggc ggcatgaacg ccatccgcga ccagatccag
      361 gaaacgtcca agcgcatcaa gcggctgggt gagtcctcgc aggagatcgg cgagatcacc
      421 gaactgattg ccgacattac cgaacagacc aacgtgctgg ccctgaacgc ggccattcag
      481 gcggcgtccg cgggcgaagc cgggcgcggc ttctcggtgg tggctgagga agtgcagcgg
      541 ctggccgaac gttccggtga tgcgacgcgc cagatcgcgg cgctggtgaa ggcgattcag
      601 accgataccc aggacgcggt ggcggccatg gagcgctcca cccagggtgt ggtcgaggga
      661 gcgcgtctgt ccgacaacgc gggcaccgca ctgtcggaga tcgaccgcgt gtcgcgtcag
      721 ctggccgagc tgatcgaacg gatttctgct tctgcctcca aggaggccga tcaggccaac
      781 gaggttgccg gcaacatcca gcacatcttc gcggtgaccg aacagacggg ggaaggtaca
      841 cgcaccacgg ccacccaggt gcgcgaactg gctcgcatgg ccgacgaact gcgtcagtcg
      901 gtggcgcgtt tcaagatcgc ctgaccgatc gcgggcatgg ggcggcggat caggttccgg
      961 cgcgcgcctg tacgagactg ctactggagt gcacgacatc atggtgatga gtcccgcagc
     1021 cgaaatgcgc gacagtggcg cgtctgccgt cagggatctg gggccgctgg cctgggtgct
     1081 ggacgaaacc cgcaggtcga tcgaagcggc cagcaagtcg ctcaagcgtt ttgcgcgtga
     1141 aaccgatgcg gcgcgcggca tcgatctggg gtcggtggac gccagtccgc tgcgcatggc
     1201 gcgccagcag cttcaccagg tggctggcgc gctggacatg gtcgaccagt cggtggcggc
     1261 actgatggtg cgtgggatgg agggtgcagt tcagcagttt gtgcagcggc ccgacaaatg
     1321 caccgaggaa accgcacagg cccttgagcg cgccggtttc gcgctcatgg aatacctgga
     1381 agcccagatg gggtcgcacc cacgctcggc ggttgggctg tttccgcaat accgccgagt
     1441 ccaggaactg gcgggcgccg atcgcatcca tccggcggat ctgtggtcgg tgccctggag
     1501 ttgggccgat ccggtggtgc ctgcatcggc gcagccgttg tcctacgacc ccgaagtgcg
     1561 ccaccgactg gatcagcgcg tactcaagat cttgcggcat cctgctgccc acgcggccga
     1621 cgaactcgcc gtcatcagcc tgagcctcgc ggccggccag atgcagcggc aggcgaccgt
     1681 gttctggctg ctgtctgcag gcttcttcga ggccctgggg cgtggattga ttgtcaagga
     1741 cgtctatgcc aagcgggcgg cgtcacgcat cctgatgcaa tacacctcgc tggcacgcgg
     1801 tggtcaggtg gtgtccgagc gattggcgca tgacttgctg ttcttctgtt cgcaggccag
     1861 gttcgatgct gatgcgaaca ccccggcact gcaggccgtg cgcaaggctt ggtcgcttga
     1921 tgcgcagcag gcgacgtcgt acgagcaggc catgtttgga ctttacgacc cggcgatact
     1981 ggcccaggcg cgtcgccgga tcgagtcggt caaggaaaac tggtcggccc tgaccggcgg
     2041 tgatctggcc cgcatgaagg cgtgcgtcga ccagttcagc ctggtggccg agtcgctcga
     2101 caagctgcac gagcagggat cggtgcttgg tgaggtattg aaccgagtgg cccagcagac
     2161 cgcgcaatcg gggcgacctc ctggcccgga actggcgatg gaaaccgcaa ccgcggtttt
     2221 gtttctggag gcttcgttcg ccgatttcca cccgggcgat gccatgtttt ccacccgcct
     2281 ggcccagttg gccgcgcggc tcgatgcggt tcgtgccggc gcatcagcgc caccgctgga
     2341 gccgtggatg gaggagctgt accgcaacgt cagcgatcgc cagaccatgg gcacggtggt
     2401 gggcgagttg cgcaccaccc tgggtgaggt ggagcatctg ctggatcagt tcttccgtca
     2461 acccgctgac cgcagcacgc tggtgtcggt gccgggcctg atggggcaga tgcgtggggt
     2521 gctgtcggtg ctgggcctgg atcaggctgc cctggcagtg ggccgcatgc gcgatctggt
     2581 cgacgaactg ctggccgcgc agcccgacac caagttggcc gacagcgcgc cttcgttcga
     2641 aaagctcggg gacaccctgg gcgcgttggg gtttcagatc gacatgctga gctaccaacc
     2701 ggtgctggca cgcaagctgt tcgtgtatga cgccgatgcc ggcgaactca ggtatcggtc
     2761 aggccgcgcc gcaactcagg tcacgacgga gcagaattcg gtggcctcgg ccaccaccga
     2821 catcgatctg ctgcttgacg ccggcaccga actgacggtg ccgacatcgg ggcccggctc
     2881 cctggtggac gaatcgctgc catccgaaac catctctggc cagcttgacg agatcgccac
     2941 catggcagcc ctggccgagc agcgggctct tgccgacgcg gcgcagcgcg ctgccgacgc
     3001 ggcgcgccgt cacgacgaag ctggcctgag cggcgcgctg gatgccctgc aggcactcac
     3061 cgctgcatcg acgccgcagc ccacgtccac gaccgtcgaa ggcggcggtg cggatgatga
     3121 tgatctgcag gccattttcc ttgaggaagc gcagcaggtg atggacaccg ggtgcgaagt
     3181 cgtgcaggcg ctgttgaccg cgcccggtga catggagctg cagaccgcct tgcgtcgtgc
     3241 gttccatacc ctgaagggca gttctcgcat ggtcgggttg accgagtttg gcgaggccgc
     3301 ctgggccatg gagcagctgt tcaatgcctg gctgcccgag cagaagccgg tgaccgagga
     3361 catgcgaggg ttggcacttg aagcactgca aggcctgcac gcctggacgg aagccatcgc
     3421 tggtcggaac gaccacggtt ggaccagcgc gccgttccgg caggcagccg atgccctgcg
     3481 actcagcggt caacgtgtgt cgctgagctg ctggcccgat gctcaggtcg ttgtggacgc
     3541 aggcgccggc catgcggatg tggtcgggga cggcgccgcg cctgccgagc agccgcatga
     3601 ggtgtcgtcg gagtccattc cggcggtgca accggcgcca cctggagagc ggggtctgcc
     3661 ggatccacag gtgcccgagt tggctgaggg tgtcgtcgaa gttgacgcca cgccagcgga
     3721 tgtggcttcg gagttggagc agcagcccgc gatcgagctg acggaactga atctcgatgc
     3781 tggtgacgac cccgatgccg gttccggcct cgaagtcatc gacctgagcg tgagtcgatg
     3841 gggcccgata tccgaaacac ccaagccgca ggggctagcg agcggcgaac ccacgcaacc
     3901 acccgttgag ctcgagctcc ccagcgcggt cgacgtgcag atgcccgtgg cattctggcc
     3961 agccaccgag acggtggaat cgatcccgcc ggtgggtggg atggtggggg atgaagcggc
     4021 gctgccggag gcgacgctgg tcagcccgga gggtctgagc gcgccacagg agggtgccga
     4081 agatgggcag actgccgacg tcgctgcgca ggacgccgag ctctaccgtc agatcgggga
     4141 cctgcgcgtc agcactgcgc tctacaacgt gtacctgaat gaggccgacg agtggtcgcg
     4201 gcaactggca accgacctgt cggagtgggc gctggagctt gatcggccgg tgcccgacgc
     4261 tgccgtcgcg cgcgcccact cgctggcggg cagctcggcc acggtgggtt tcgctgcgct
     4321 gtcggagctg gcgcgtgcgg tggagcacgc cctgcaacgt ctggtcgggc aatcgcgcgg
     4381 cacgaccgag caggcgcaat gtctcaacga agcggcgcag gacatccggc gtgtgctgca
     4441 tcagtttgca gctggcatgc tgaagactcc gaacccggac atccttgcgg cggtgcaggc
     4501 gctggaaccg gtggttgccg gcgacgaagt cgctccggcg ctggcgcaag cgtcggcctc
     4561 gacctggatg gatcttgccg agacggtgcc cttgtcgtcc acgaaagacg aagctcagcc
     4621 gccggtggtc acccgggatt cgttgcagtc gctgcgtgcc ctgggtgacg atatcgacgt
     4681 cgccgacgtg atcgatcccg acctgttccc catctttgag gaagaggcgg aagaacttct
     4741 gccgcagatg ggcacggcct tgcgccgctg ggtggcgcac ccggacgatc gtgaggcacg
     4801 tgctcaggcc ctgcgtgccc tgcacacgct caagggcagc gcacggctcg ccggcgcgtt
     4861 gcggctgggc gagctggcac accggacgga atcggccgtc gagaccctgg ggctggacca
     4921 gctcgtttcc gccgacctgg agccgctgct ggatcacttc gatgagttgc aggctgggtt
     4981 ccgccggctg caggcgggcg agcagggcgt ggagaccagc caggtggcgt cggtgccgga
     5041 tgttgccacg gcggaagatg caaggccggc ggaggagccg tctgccgagg ccggtcaggg
     5101 cgagtcgcgc gtcgtcgtgc cgacggtcac acggggggac atcgcttcgc cggccgcgct
     5161 gtcgctggtg tcggcacgcg ccgccgccag ccaggcggtg cgggtgcgct cacagctgct
     5221 cgatcggctg gtgatgcagg ccggcgaggt ggccacctcg cgcgcccggc tggaagccga
     5281 aatcggccag ttgcgcgggc tgctggggga gctgaccggc aacctcgacc gcctgcgtgg
     5341 ccagttgcgc gacgtcgagc tgcaggccga gacgcagatg cagtcgcggc tggcgctgtc
     5401 ccgggacgcg cagcagaact tcgatccgct ggagttcgac cgattcacgc gcatgcagga
     5461 actcacccgc atgatggccg agtcggtcaa cgacgtcgcc accgtgcagc gcagcctgca
     5521 gcgcgccgtc gagggcagcg aagacaacct ggcggcgcag gcgcgccagt cgcgcgagtt
     5581 gcagcgcgag ctgctgcgca cccgcatggt ggagttcgac agcattgccg aacgcctgta
     5641 tcgggtggtg cgccaagcca gcaaggaaac cggcaagcag gtgcggctcg acatcgtggg
     5701 cggcgcgacc gagatcgatc gcggcatcct ggagcgcatg acgccggcct tcgagcacct
     5761 ggtgcgca
//
LOCUS       DAOOOG010000161         5751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_16759_length_5751_cov_26.794944, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000161 DAOOOG010000000
VERSION     DAOOOG010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16759_length_5751_cov_26.794944"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1955
                     /locus_tag="PK242_10785"
     CDS             <1..1955
                     /locus_tag="PK242_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOZ94547.1"
                     /translation="TATRTNLEAADEVARQMRLRDLGGLIVIDFIDMEESKNRREVEN
                     RLRDALRQDRARVQFGSISKFGLLEMSRQRLKPALSEGASIPCPRCGGSGHVRDTESS
                     ALQILRIIQEESMKDNTAAVHVQVPVEVASFLLNEKRPEIAKIELKQRVNVTLVPNKT
                     LETPHYKLERLKHDDPRLDDIDSSYKLAEEFEDPTQVTRRSQEPTNRQQPVIKGVLPD
                     VPAPQPVARPEPAPVAAAAPAAAPVVTPAPQSGGLMGWLKNLFGVAPPPAPVAAPAPA
                     PAAAGEDRKDARTGGREGERRGGRGGRGASRDGSGRRQDGRGNGNGGASAQAEGRTGP
                     REGGRRGNERGERGERPERGERQGPRERRDALPADAGASNPVSDTVTTDSGSDASGRP
                     PRAERGDGRRGRRERSDAAPVEGVTAAASGPRPEGEAAAVAPDAGPTEERRERRPRGE
                     RGERGERGERGGRERRERDAEAREPRAAELPGAMSADGSVAPATSAPSTDAPAPRSYF
                     ARAAEAPAVVEQESAPAPVEPAPAATLSVANEAAPRATVSDAPRRHGLPRVTPFSLPL
                     GELDALAQAAGLQWVNSDADKVASVQAAIAAEPQPIHVPRERPPRVVLDDGPLVLVET
                     RLDLRQVQLPFESATPPATDSAPAPR"
     gene            2276..2845
                     /gene="ahpC"
                     /locus_tag="PK242_10790"
     CDS             2276..2845
                     /gene="ahpC"
                     /locus_tag="PK242_10790"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017114658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HOZ94548.1"
                     /translation="MSLINTKVQPFKAQAFHNGKFEEVTEKNFSNGKQWSVVIFMPAA
                     FTFNCPTEIEDAAEHYAEFQKMDAEVFIVTTDTHFSHKVWHETSDAVGKAKFYLVGDP
                     THQLTNNFGVHIPEEGLALRGTFVIDPSGTIKTMEVHSNEIARDVSETVRKLKAARYT
                     AAHPDEVCPAKWKEGEKTLKPSIDLVGKI"
     gene            3095..4684
                     /gene="ahpF"
                     /locus_tag="PK242_10795"
     CDS             3095..4684
                     /gene="ahpF"
                     /locus_tag="PK242_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HOZ94549.1"
                     /translation="MLDQDIKQQLAAYLERVQEPFEMVATLGTDAKSAELRELLEEVA
                     SIRPDKISLRTDGSDARVPSFTLARPGSGTQLRFAAIPMGHEFTSLILALLWTGGHPP
                     KVEADVLEQIKALDVDLDFEVYMSLSCHNCPDVVQALSLISIYNPRIKTTIIDGGMFQ
                     KEIEAREIMGVPAIYQGGQMFHSGRITLEEILQKIDTGAAKRDAAKLSAKAPYEVLVI
                     GGGPAGAAAAIYTARKGLRTGIVADRFGGQVNDTLDIDNYPGVPHTTGPEYAAQLAAH
                     VADYDIDVMNAQRAAELVPAAQPGGLVTVKLDNGGQLQSRTVILATGARWRNVGVPGE
                     DEYRTKGVAYCPSCDGPLFKGKDVAVIGGGNSGIEAAIDLAGVVKHVTVLEFMPELKA
                     DAVLVKKLHSLPNVTVHTNAQTTEITGADGKVNGLAFKHRGDGTDARVELEGVFVQIG
                     LVPNTEWLGQSVERTKFGEIVVDARGATNVPGVFGAGDCTTVPYKQIVVAAGAGSTAA
                     LSAFDYLIRTPAPAEPRAVAA"
     gene            complement(4753..5604)
                     /locus_tag="PK242_10800"
     CDS             complement(4753..5604)
                     /locus_tag="PK242_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ94550.1"
                     /translation="MRLDLSVPTPLAYFASLVQRDAELPLLEAAACLAQDASPELDVQ
                     QVLHEVDRLAERLLRQVTRGAAEIERLRGLNRFFFEELGFAGNVNDYYNPDNSYVHRV
                     LHTRRGIPISLAVLWLELARGLGLSAHGLGFPGHFLVQVELPDGLVVLDPFTGQSLGL
                     VELRARIDEFQPQGGARSGDVLPLAMHLRPARPRQILARMLRNLQEIHRTQRDWARLV
                     QVQDRLVTLLPDAWPEYRERGLALAELGQTARAQADLNLYLQHASDASDRARVAERLG
                     ALPRSRS"
BASE COUNT         1020 a         2105 c         1902 g          724 t
ORIGIN      
        1 aaaccgccac ccgcaccaac ctcgaagccg ccgacgaagt ggcgcgccag atgcgcctgc
       61 gcgacctggg cggtctgatc gtgatcgact tcatcgacat ggaggagagc aagaaccgcc
      121 gcgaggtcga aaaccgcctg agggacgcgc tgcgccagga ccgcgcgcgc gtgcagttcg
      181 gctccatcag caagttcggc ctgctggaga tgagccgcca gcgcctcaaa cccgccctga
      241 gtgaaggtgc ctccatcccc tgcccgcgct gcggcggctc gggccatgtg cgcgacaccg
      301 aatccagcgc gctgcagatc ctgcgcatca tccaggaaga gtccatgaag gacaacaccg
      361 ccgccgtgca cgtgcaggtg ccggtggaag tggccagctt cctgctgaac gaaaagcgcc
      421 ccgagatcgc caagatcgag ctcaagcagc gcgtcaacgt caccctggtg cccaacaaga
      481 cgctggagac gccgcactac aagctcgaac gcctcaagca cgacgacccg cgcctggacg
      541 acatcgattc cagctacaag ctggccgagg agttcgaaga cccgacccag gtcacgcgcc
      601 gcagccagga gccgaccaac cgccagcagc ccgtcatcaa gggcgtgctg cccgacgtgc
      661 cggcaccgca gccggtggcc cggcccgagc cggcgcctgt ggccgcggct gcgcccgccg
      721 ccgcgcccgt ggtcacgccc gcgccgcagt ccggcggcct gatgggctgg ctcaagaacc
      781 tgtttggcgt ggcgccgccg ccggcccccg tggcggcccc ggcgcccgcc ccggccgccg
      841 ccggcgaaga caggaaagac gcacgcaccg gtggccgcga gggcgaacgc cgcggtgggc
      901 gcggcggccg tggcgcatcg cgcgacggca gcggccgtcg ccaggacggc cgcggcaatg
      961 gcaacggtgg cgcgtctgcg caggccgagg gccgcaccgg cccgcgcgaa ggcggccgcc
     1021 gcggcaacga gcgcggcgaa cgcggtgaac gccccgagcg cggcgaacgc cagggtccgc
     1081 gcgagcgccg cgacgccctg ccggccgacg ccggcgccag caacccggtc agcgataccg
     1141 tcacgaccga cagcggcagc gacgccagcg gccgcccacc ccgtgccgag cgcggcgacg
     1201 gccgccgcgg ccggcgcgag cgcagcgacg cagcgcctgt ggaaggcgtg accgccgccg
     1261 ccagcggccc gcgcccggaa ggcgaagccg ccgccgtggc accggacgcc ggcccgaccg
     1321 aggagcggcg cgaacgccgc ccgcgcggtg aacgcggtga acgcggtgaa cgcggtgaac
     1381 gcggtggccg tgagcgccgc gagcgcgacg ccgaggcgcg cgagccgcgc gcggccgagc
     1441 tgcccggcgc catgtcggcc gatggcagcg ttgcccccgc caccagcgcg ccgagcaccg
     1501 acgccccggc gccgcgctcg tacttcgccc gtgccgccga agcacccgcc gtggtggagc
     1561 aggaatcggc tccagcgccc gtcgaaccag cgccagcagc tacgctttct gtagcaaacg
     1621 aggcagcacc ccgagccacc gtgagcgacg caccgcgccg ccacggcctg cccagggtga
     1681 cgccgttcag cctgccgctg ggcgagctgg acgcgctggc ccaggccgcc ggcctgcagt
     1741 gggtcaactc ggacgccgac aaggtggcca gcgtgcaggc ggccatcgcc gccgagccgc
     1801 agccgatcca cgtgccgcgc gagcgcccgc cgcgcgtggt gctggacgac ggcccgctgg
     1861 tgctggtcga aacccgcctc gatctgcgcc aggtgcagct gccgttcgag tccgccaccc
     1921 cgcccgccac cgactcggcg cccgcccccc gctgatcgcc ctgcccgccg ccgccccgcc
     1981 cgggggtggc gcgtgtggct ccacggcgcg gctctgccgg gcctggtgcg gcttggcctt
     2041 gtggcgcggc tgccctggcg cgcagaccag ccacttctca tgtaagctga cggcccccaa
     2101 gcgcgagtcg gccgccgggc gacgccctgg ggcgcgtccc agcggcgcca gccgcagcga
     2161 cgggcaccga taggcgccgg ctaaaaatag attagacaaa atgaatcacg tattcaatag
     2221 ttttgatcta tagtttcacg catcgcatcc atttcatcaa ccaaggagca cacgcatgtc
     2281 cctgatcaac accaaggtcc agcccttcaa ggcccaggct ttccacaacg gcaagttcga
     2341 agaagtcacg gaaaagaatt tctccaacgg caagcagtgg tcggtcgtca tcttcatgcc
     2401 cgccgccttc accttcaact gcccgaccga gatcgaggac gcggccgagc actacgccga
     2461 gttccagaag atggacgccg aggtgttcat cgtcaccacc gacacgcact tcagccacaa
     2521 ggtgtggcac gagacttccg acgcggtggg caaggccaag ttctacctgg tgggcgatcc
     2581 cacgcaccag ctgaccaaca acttcggcgt gcacatcccc gaagaaggcc tggcgctgcg
     2641 cggcaccttc gtgatcgacc ccagcggcac catcaagacc atggaagtgc actccaacga
     2701 aatcgcccgc gacgtcagcg agacggtgcg caagctcaag gccgcgcgct acaccgccgc
     2761 ccacccggac gaggtctgcc cggccaagtg gaaggaaggc gagaagacgc tgaagccgtc
     2821 gatcgacctg gtcggcaaga tctgagcgcg cgcggctact gcgcgaaccg attgccgcgt
     2881 tggccggtgc tcgcaatcct cacgtaccgc aaatacgttc cggttgctgc gcgccgtccg
     2941 ccttgcactc ggtccgctcg ctacgccgcc tgaccgcctg cgaccgaacg gcgacagggc
     3001 atgcctgccc tgccgttcgg ttttttattg atcaaatatg cctctagcgc ttatccatca
     3061 agcgcaaaca gctatcacat caggagtaat caccatgttg gaccaagaca tcaagcaaca
     3121 actcgcggcc tacctcgagc gcgtgcaaga gccgttcgag atggtggcga ccctgggcac
     3181 ggacgccaaa tccgccgagc tgcgcgagct gctggaagaa gtggcctcca tccgccccga
     3241 caagatcagc ttgcgcaccg acggcagcga cgcgcgcgtg ccgtcgttta ccctggcgcg
     3301 ccccggtagc ggcacgcaac tgcgctttgc cgccatcccg atgggacatg aattcacctc
     3361 gctcatcctg gccctgctgt ggaccggcgg ccacccgccc aaggtcgaag ccgacgtgct
     3421 ggagcagatc aaggcgctgg atgtggacct ggacttcgag gtctacatga gcctgtcctg
     3481 ccacaactgc ccggacgtgg tgcaggcgct gtcgctcatc agcatctaca acccgcgcat
     3541 caagaccacc atcatcgatg gcggcatgtt ccagaaggaa attgaagcgc gcgagatcat
     3601 gggcgtgccc gccatctatc agggcggcca gatgttccac agcggccgca tcacgctcga
     3661 agaaatcctg cagaagatcg acaccggcgc cgccaaacgc gacgccgcca agctgagcgc
     3721 caaggcgccc tacgaggtgc tggtgatcgg cggcggcccg gccggcgcgg cggcggccat
     3781 ctacaccgcg cgcaagggcc tgcgcaccgg catcgtggcc gaccgctttg gcggccaggt
     3841 caacgacacg ctggacatcg acaactaccc cggcgtgccc cacaccactg gccccgaata
     3901 cgcggcccag ctggctgcgc acgtggccga ctacgacatc gacgtgatga acgcccagcg
     3961 cgcggccgag ctggtgcccg ccgcgcaacc tggtggcctg gtgacggtga agctggacaa
     4021 cggtggccaa ttgcaaagcc gcaccgtcat cctggccacc ggcgcgcgct ggcgcaacgt
     4081 cggcgtgccc ggcgaggacg aataccgcac caagggcgtg gcctactgcc ccagctgcga
     4141 cgggccgctg ttcaagggca aggacgtggc ggtgatcggc ggcggcaact cgggcatcga
     4201 ggccgccatc gacctggccg gcgtggtgaa acacgtcacc gtgctcgagt tcatgcccga
     4261 actcaaggcc gacgccgtgc tggtgaagaa actgcacagc ctgcccaacg tgaccgtgca
     4321 caccaacgcc cagaccaccg agatcaccgg ggccgacggc aaggtcaacg gcctggcctt
     4381 caagcaccgc ggcgacggca cggacgcccg ggtcgagctg gaaggcgtgt tcgtgcagat
     4441 cggtctggtg cccaacaccg agtggctggg tcaaagcgtc gagcgcacga agttcggcga
     4501 gatcgtggtc gatgcgcgtg gcgccaccaa cgtgccgggc gtgttcggcg ccggcgactg
     4561 caccaccgtg ccctacaagc agattgtggt ggcggcgggc gccggctcga ccgccgcgct
     4621 cagcgccttt gactatctga tccgcacgcc ggcgccggcc gagccgcggg ccgtggccgc
     4681 ctgagcaccg cggtcggacc cggccttcca gcccgctgca tcagcgggct tttttgcgcc
     4741 ccggggtcgg actcatgacc gcgaacgcgg cagcgcgccc agccgctcgg ccacacgcgc
     4801 gcggtcgctg gcgtcgctgg cgtgctgcag gtacaggttc aggtcggcct gggcgcgtgc
     4861 agtctggccc agctcggcca gggccaggcc gcgttctcgg tattccggcc aggcgtcggg
     4921 cagcagggtc accagccggt cctgcacctg caccaggcgc gcccagtcgc gctgggtacg
     4981 atggatctcc tgcaggttgc gcagcatgcg cgccagaatc tgacgcgggc gggccgggcg
     5041 cagatgcatg gccagcggca gcacatcacc ggaccgcgcg cccccctgcg gctggaattc
     5101 gtcgatgcgg gcacgcaact cgaccaggcc cagcgactgg ccggtgaaag gatccagcac
     5161 caccaacccg tcgggcagct cgacctgcac caggaaatgc cccggaaacc ccagcccatg
     5221 agccgacagc cccaggccgc gagccaactc cagccacagc acggccagtg agatggggat
     5281 gccgcgccgc gtgtgcagca cccggtgcac gtagctgttg tccgggttgt agtagtcgtt
     5341 gacgttgcct gcgaacccca gctcctcgaa gaagaagcgg ttcagcccac gcagccgctc
     5401 gatttccgcg gcgccgcgcg tcacctgccg gagcagccgc tcggccagcc gatcgacctc
     5461 atggagcacc tgctgcacat ccaactcggg gctggcgtcc tgtgccaggc aggccgcggc
     5521 ctccagcagc ggcagctccg cgtcgcgctg caccaggctg gcgaaatacg ccagcggcgt
     5581 ggggacggac agatcaaggc gcatgacgat cttgtaaggg cgcggcgcgg gccacgtcaa
     5641 ggccgcaggt caagcacgtc agcgccgcag caactgccgc aggcgcacgc cgctggccgc
     5701 gagggcgccg aaataaatca gtgccgcccc ggcgatccac gccgccagca g
//
LOCUS       DAOOOG010000162         5616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_17284_length_5616_cov_27.553318, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000162 DAOOOG010000000
VERSION     DAOOOG010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17284_length_5616_cov_27.553318"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PK242_10805"
     CDS             complement(<1..961)
                     /locus_tag="PK242_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOZ94551.1"
                     /translation="MQQDILINWSPQETRVAVVESGAVQELHVERALERGLVGNIYLG
                     KVARVLPGMQSAFIDIGLERAAFLHVADLWQKPVNGDGLPANRTGAPPKPIERQVFEG
                     QTLMVQVIKDPIGSKGARLSTQVSLAGRMLVFLPQDDHVGVSQKIPAAQRDELRGRVQ
                     ALVAAGGEGGRSGGFILRTNAEDASDAELAEDIAYLRKTWARIRESATRQPPATLLHE
                     DLSLLQRVLRDLTSEATQSIRIDSREQFDGLLAFGQEFMPAAAARLSHYKGERPIFDL
                     FNVDEEITKALGRRVDLKSGGYLIVDQTEALTTIDVNTGGFVGA"
     gene            complement(984..1577)
                     /locus_tag="PK242_10810"
     CDS             complement(984..1577)
                     /locus_tag="PK242_10810"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOZ94552.1"
                     /translation="MSFIYLASQSPRRRQLLDQLGVAHRLLLPDDAAAAEALEAVRPG
                     EAPAAYVRRVTRLKLDAALRRLHQQGLPPAPVLCADTTVALGRHILGKPADAREAAAM
                     LRQLAGGTHRVITALALQAGERRHEALSDSRVRFAPVSETELADYLASGEWQGKAGAY
                     AIQGRAAAFIAHISGSYSGIMGLPLFETHQIMRLARL"
     gene            complement(1620..2090)
                     /gene="rlmH"
                     /locus_tag="PK242_10815"
     CDS             complement(1620..2090)
                     /gene="rlmH"
                     /locus_tag="PK242_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOZ94553.1"
                     /translation="MKLIVIAVGQRLPGWAQTAWDDYAKRFPPELRLQLKAVKTEPRA
                     GGRTAPQAMAAERQRIEAALPRGAHVVVLDERGSALTTVALADRLRQWQGQGGDVALL
                     IGGPDGLDPALRQAAHERIRLSDLTLPHALVRVLLVEQLYRAWSVNAGHPYHRE"
     gene            complement(2436..3074)
                     /gene="rsfS"
                     /locus_tag="PK242_10820"
     CDS             complement(2436..3074)
                     /gene="rsfS"
                     /locus_tag="PK242_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOZ94554.1"
                     /translation="MSETAAKKDLQKLQRAIVDGLEDIKAQDIRVFNTEHLSPLFERV
                     IVATGSSNRQTKALATSVRDKVREAGFAKPRMEGEDNAEWIIVDCGQAVAHVMQPAIR
                     QYYKLEEIWGEKPVRLRNAAPLSRSSAAAPAAKKAAAAAPAAKKAATAARKTATAGTA
                     AKKAPAGKAPAKKAVATPAAPAKRAPAKKAVAGKTAAVKSAAGKPAPRRKPA"
     gene            complement(3074..3703)
                     /gene="nadD"
                     /locus_tag="PK242_10825"
     CDS             complement(3074..3703)
                     /gene="nadD"
                     /locus_tag="PK242_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOZ94555.1"
                     /translation="MTAGIHRVGLFGGAFDPPHHAHVALAQAAIEQLGLERLHVVPTG
                     DAWHKARALSPAADRLAMCRLAFAGLPRVAVDERELRRAGASYTIDTLTELHAEHPAA
                     QWFLLIGADQAAAFHSWRRADDILRLATIVIAVRADPSGVGAVFDAEHPLPGLAVAAD
                     RVRRLQLPAMPHSATAVRRLAAAGQPIEHLVPAPVAGYIADHHLYQSPA"
     gene            complement(3858..4775)
                     /gene="hemF"
                     /locus_tag="PK242_10830"
     CDS             complement(3858..4775)
                     /gene="hemF"
                     /locus_tag="PK242_10830"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HOZ94556.1"
                     /translation="MSAFSSRVHDYLSGLQSRITQAIEAADGRAVFRRDDWHKAAGEP
                     LQGAGSTRILEGGAVFERAGIGLSQVRGPRLPPSATQHRPELEGSPFEAMGVSLVFHP
                     LNPYVPTVHMNVRMISAGHPGAEPVCWFGGGMDLTPYYGFDEDCQHFHRTCRDALAPF
                     GDDTYPRFKAWCDDYFFNRHRGEARGIGGIFYDDFVGGGAEQGLALTRAVGEAFLQAY
                     LPIVERRAATPWGERERQHQLHRRGRYVEFNLVWDRGTHFGLQSGGRTESILLSMPPL
                     VAWQYQRQPEPGSPEARLTEHFLVPRAWV"
     gene            complement(4777..>5616)
                     /gene="purD"
                     /locus_tag="PK242_10835"
     CDS             complement(4777..>5616)
                     /gene="purD"
                     /locus_tag="PK242_10835"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOZ94557.1"
                     /translation="GKGVVVAQTVAEAHAAIDDMLGGNSLGVAHNEGGARVVIEEFLP
                     GEEASFIVLSDGKSVLALASSQDHKRLQDGDQGPNTGGMGAYSPAPVVSADVHARVMR
                     EVILPTVRGMEQDGIPFTGFLYAGLMIDAQGRPRTLEFNTRMGDPETQPILMRLKSDL
                     VEVLLAATAGRLHEVELQWDRRVALGVVLAAAGYPLAPRKGDPITGLPAPQEDVRVFH
                     AGTVADGGQVRTAGGRVLCVTALADSVRQAQARAYDAVRGIHFEGMQYRHDIGHHAIR
                     QNP"
BASE COUNT          777 a         1947 c         2004 g          888 t
ORIGIN      
        1 gcgcgccgac gaagccgccg gtgttgacgt cgatggtggt cagcgcctcg gtctgatcga
       61 cgatcaggta gccgcccgat ttcaggtcga cgcgccgacc cagcgccttg gtgatttcct
      121 cgtcgacgtt gaacaggtcg aagatcggcc gctcgccctt gtagtgcgac aggcgcgccg
      181 cggcggccgg catgaactcc tggccgaagg ccagcaggcc atcgaactgc tcgcgcgaat
      241 cgatgcggat cgactgcgtg gcctcgctcg tcagatcgcg cagcacgcgc tgcagcaggc
      301 tcagatcctc gtgcagcagc gtggccggcg gctggcgcgt ggccgactcg cggatgcgcg
      361 cccaggtctt gcgcaggtag gcgatgtcct cggccagttc ggcgtcgctg gcgtcttcgg
      421 cgttggtgcg cagaatgaag ccgccgctgc ggccgccctc gccacccgcg gccaccagcg
      481 cctgcacgcg cccgcgcagt tcgtcgcgct gggctgccgg gatcttctgc gacacgccga
      541 catgatcgtc ctgcggcaga aacaccagca tgcgcccggc caggctgacc tgcgtcgaca
      601 ggcgcgcgcc cttgctgccg atcgggtcct tgatgacctg caccatcagc gtctggccct
      661 cgaacacctg cctttcgatg ggtttggggg gcgcgccggt gcggttggcc ggcaggccgt
      721 cgccattgac gggcttctgc cacaggtcgg ccacgtgcag gaaggccgcg cgctccagcc
      781 cgatgtcgat gaaggccgac tgcatgcccg gcagcacgcg cgccaccttg cccaggtaga
      841 tgttgccgac caggccgcgc tcgagcgcgc gctcgacgtg cagctcctgc accgcgccgc
      901 tttcgaccac cgccacgcgg gtttcctggg gtgaccagtt gatgaggatg tcttgctgca
      961 tttggtatag ctgctggcgc tttctacaag cgcgccagac gcatgatctg atgggtttcg
     1021 aacaggggca ggcccatgat gccgctgtag ctgccgctga tgtgggcgat gaaagccgcc
     1081 gcccgcccct ggatggcata ggcgccggcc ttgccctgcc attcgccgct ggccaggtag
     1141 tcggccagct cggtttcgct cacgggcgca aagcgcaccc gtgaatcgct cagcgcctcg
     1201 tggcgccgct cgcccgcctg cagcgccagc gccgtaatca cgcgatgcgt gccaccggcc
     1261 agctggcgca gcatggccgc cgcctcgcgc gcgtcggccg gcttgccgag gatgtggcgg
     1321 cccagcgcca ccgtggtgtc ggcgcacagc accggcgccg gcggcaggcc ctgctggtgc
     1381 aggcggcgca gcgcggcgtc gagcttgagc cgcgtcacgc gccgcacgta ggcggccggc
     1441 gcctcgcccg ggcgcaccgc ctccagcgcc tcggcggcgg ccgcgtcatc gggcagcagc
     1501 aggcgatgcg ccacgccgag ctgatcgagc agctggcgcc ggcgcggact ttgcgaggca
     1561 aggtagatga agctcatgca acaggcgaag cgacggccgt cggtggcgcg gccgggcgct
     1621 cattctcggt gatagggatg gcccgcgttg accgaccagg cgcggtagag ctgctcgacc
     1681 agcagcaccc gcaccagggc gtgcggcagc gtcaggtcgg acaggcgaat gcgttcatgc
     1741 gccgcctggc gcagggccgg gtccagccca tcggggccgc cgatcagcag cgccacgtcg
     1801 ccgccctggc cctgccactg gcgcaggcga tccgccaggg ccacggtggt gagcgcgctg
     1861 ccgcgctcgt ccagcaccac cacatgggca ccacgcggca gcgccgcctc gatgcgctgg
     1921 cgctcggccg ccatggcctg tggcgcggtc ctgccaccgg cgcgcggctc ggtcttgacg
     1981 gccttgagct gcagccgcag ttcgggcgga aagcgcttgg cgtagtcgtc ccaggccgtc
     2041 tgcgcccagc cgggcaggcg ctggccgacg gcaatgacga tcagcttcat ggctgctcac
     2101 gacgcatcgc gttcaacaca cccgacccgc gacgccgctg gcgctggtgc agtgtttcgc
     2161 tctgtcaggc acttatggaa gccagaatga ggcttgcggc caggcgcggc tcgcagccca
     2221 tgctggcggc cttggcaaga ggcgcaacgc cgccgcaagc gtttttcggg cttccccctt
     2281 ggcgactcgc tgtaggtacc tgacagcgcg aaacactgca ccagcaagac gaacaaaccg
     2341 atggtcatcg aagcgcagca acgtggttgg ccatcggtca ggctgggcgc gagttggtcg
     2401 caggccgctg accgcaggca gggggcaaac ggggatcagg ccggctttct gcgcggggcg
     2461 ggcttgccgg cggcagactt gaccgcggcg gtctttccgg ccacggcctt cttggcaggt
     2521 gcccgcttgg caggcgccgc cggcgtggcg acagccttct tggcgggggc tttcccggcc
     2581 ggtgccttct tggccgccgt gcccgcggtt gcggtcttcc gggcggcggt cgctgccttc
     2641 ttggcggccg gggcggcggc ggccgccttc ttcgcggccg gggcagcggc ggacgacctg
     2701 gacagtggcg ccgcgttcct caggcgcacc ggcttttcgc cccagatctc ttccagcttg
     2761 tagtactggc gaatggccgg ctgcatgaca tgggccaccg cctggccgca gtcgacgatg
     2821 atccattcgg cgttgtcttc gccttccatg cgcggcttgg caaaaccggc ttcacgcacc
     2881 ttgtcgcgca cgctggtggc cagcgccttg gtctggcggt tggagctgcc ggtggcgacg
     2941 atcacccgtt cgaacagcgg cgacaggtgt tcggtgttga acacgcggat gtcctgggcc
     3001 ttgatgtctt ccaggccatc gacgatggcg cgctggagtt tctgcaggtc tttcttggcg
     3061 gcggtttcgc tcatcaggca gggctttggt agaggtggtg gtcggcaata taccccgcga
     3121 ccggcgcggg aaccaagtgt tcgatcggtt gccccgccgc cgccaggcgg cgcacggcgg
     3181 tggcgctgtg cggcatggcg ggcagttgca gccgccgcac gcggtccgcc gcgacggcca
     3241 ggccgggcag cgggtgttct gcatcaaaaa cggcgccgac gcccgaggga tcagcgcgaa
     3301 cagctatcac aatggtagct aaccgaagga tgtcgtcggc ccgtcgccag ctgtgaaagg
     3361 ccgccgcctg gtcggccccg atgagcagga accactgtgc cgcggggtgt tcggcgtgca
     3421 gctcggtcag ggtatcgatg gtgtagctgg cgccggcgcg ccgcagttca cgttcgtcca
     3481 ccgccacccg gggcaggccg gcaaaggcca gccggcacat ggccagccgg tcggcggccg
     3541 ggctgagcgc gcgcgccttg tgccaggcat cccccgtggg caccacgtgc aggcgctcca
     3601 gccccagttg ctcgatcgcc gcctgcgcca gcgccacatg ggcgtggtgc ggcgggtcga
     3661 aagcgccgcc gaacaggccg acacggtgga tgccggcggt catgacgggt cgatgaccaa
     3721 tgacttcgcc gctggcgcgg cttcaatgac caatgacctt gccctcgcgc cactctgcgc
     3781 gcggtccccg gcatccgaca ggctctgatg tgtcattggt catcgaatcg aagcgaggtc
     3841 attcgtcatc gcgtgcgtca cacccacgcg cgcggcacca ggaaatgctc ggtcaggcgc
     3901 gcctcgggcg aaccgggctc gggctggcgc tggtactgcc aggccaccag cggcggcatg
     3961 gacagcagga tggattcggt gcgcccgccc gattgcaggc cgaagtgcgt gccgcggtcc
     4021 cacaccaggt tgaattcgac gtagcgcccg cgccggtgca gctggtgctg gcgctcgcgc
     4081 tcgccccagg gggtggcggc gcgccgctcg acgatcggca ggtaggcctg caaaaaggcc
     4141 tcgcccaccg cgcgcgtgag ggccagaccc tgctcggcgc cgccgcccac gaagtcgtcg
     4201 tagaagatgc cgccgatgcc gcgcgcctcg ccgcggtggc ggttgaagaa gtagtcgtcg
     4261 caccaggcct tgaagcgtgg gtaggtgtcg tcgccgaagg gagccagggc gtcgcggcag
     4321 gtgcggtgga aatgctggca gtcttcgtcg aagccgtagt acggcgtcag gtccatgccg
     4381 ccgccgaacc agcacaccgg ttcggcgccc gggtggccgg ccgagatcat gcgcacgttc
     4441 atgtgcaccg tgggtacgta ggggttgagg gggtggaaca ccagcgacac gcccatggcc
     4501 tcgaacgggc tgccttccag ctcgggccga tgctgggtgg ccgacggcgg caggcgcggg
     4561 ccgcgcacct gcgacaggcc gatgccggcg cgctcgaaca ccgcgccgcc ctccagaatg
     4621 cgggtgctgc ccgcgccctg cagcggttcg ccagcggcct tgtgccagtc gtcgcgcctg
     4681 aacacggccc gaccgtcggc ggcctcgatg gcctgggtga tgcgcgactg caggccgctc
     4741 aggtagtcgt gcacccggct ggaaaaagcg ctcatgtcag gggttctgcc ggatcgcgtg
     4801 gtggccgatg tcgtgccggt actgcatgcc ctcgaaatgg atgccgcgca ccgcgtcgta
     4861 ggcgcgcgcc tgcgcctgcc ggaccgagtc ggccagcgcg gtgacgcaca gcacacgccc
     4921 accggcggtg cgcacctgcc cgccgtcggc caccgtgccg gcgtgaaaca ccctgacgtc
     4981 ttcctgcggc gcgggcaggc cggtgatcgg gtcgcccttg cgcggcgcca gcgggtagcc
     5041 cgcggcggcc agcaccacgc ccagcgccac gcgccgatcc cattgcagct cgacctcgtg
     5101 cagccggccg gcggtggcgg ccagcagcac ctcgaccagg tcggacttca ggcgcatcag
     5161 gatgggctgg gtttcggggt cgcccatgcg ggtgttgaat tccagcgtcc tgggccggcc
     5221 ctgggcgtcg atcatcaggc cggcgtacag aaagccggtg aaggggatgc cgtcctgctc
     5281 catgccgcgc accgtgggca ggatcacctc gcgcatgacg cgtgcgtgca cgtcggcgct
     5341 gaccaccggg gccggtgaat aggcgcccat gccgccggtg ttggggccct ggtcgccgtc
     5401 ctgcaggcgc ttgtggtcct ggctgctggc cagtgccagc acgctcttgc cgtcggacag
     5461 gacgatgaag ctggcttcct cgcccggcag gaattcttcg atgaccaccc gcgcgccgcc
     5521 ttcgttgtgc gccacgccca ggctgttgcc gcccagcatg tcgtcgatgg cggcgtgcgc
     5581 ctcggccacg gtttgcgcca ccaccacgcc cttgcc
//
LOCUS       DAOOOG010000163         5568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_17490_length_5568_cov_26.630328, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000163 DAOOOG010000000
VERSION     DAOOOG010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17490_length_5568_cov_26.630328"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PK242_10840"
     CDS             complement(<1..847)
                     /locus_tag="PK242_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ94558.1"
                     /translation="MSYKAPVKDLLFNIEHLARIDQIAAIPGFEDAGLETAQAVLEEC
                     ARFNEGVVAPLNFEADKAPSSHSGTSVTTSAGFKEAYRQYIEGGWQGLQHPAAFGGQG
                     LPKTIGAACGEVLNSANLSFALCPLLTDGAIEALLTAGSEELKATYLEQLVSGHWTGT
                     MNLTEPQAGSDLAAVRTRAEPQPDGTYRIFGTKIFITYGEHDMAENIVHLVLARVQGA
                     PEGVKGISLFVVPKFMVKPDGSLGERNDVYCVSIEHKMGIKASPTAVLQFGDHGGAVG
                     YLVGEE"
     gene            complement(1114..2046)
                     /locus_tag="PK242_10845"
     CDS             complement(1114..2046)
                     /locus_tag="PK242_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOZ94559.1"
                     /translation="MTALVIAEHDNASIKGATLNTVTAAAACGGDVHVLVAGHNAAAA
                     AQAASQIAGVAKVLHADAPALAHGLAENLAAQVLAVAGNYSHILFPATAGGKNAAPRV
                     AARLDVAQISEVTKVDSPDTFERPIYAGNAIATVQSADATKVITVRATGFDAAAASGG
                     SAAVESVAAVADSGKSGFVGQEIAKSDRPELTAAKIVVSGGRALGSAEKFNEVMTPLA
                     DKLGAAIGASRAAVDAGYAPNDLQVGQTGKIVAPQLYIAAGISGAIQHLAGMKDSKVI
                     VAINKDEEAPIFSVADYGLVADLFEAVPALTQAL"
     gene            complement(2176..2928)
                     /locus_tag="PK242_10850"
     CDS             complement(2176..2928)
                     /locus_tag="PK242_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOZ94560.1"
                     /translation="MKALVAVKRVVDYNVKVRVKSDGTGVDIANVKMSMNPFDEIAVE
                     EAVRLKEKGVLTEVVAVSCGVAQCQETLRTAMAIGADRAILVETDAELQPLAVAKLLK
                     ALVDKEQPGLVILGKQAIDDDANQTGQMLAALAELPQATFASKVEIADGKASVTREVD
                     GGLETLALTLPAVVTTDLRLNEPRYVTLPNIMKAKKKPLDTVKPEDLGVDVAPRLKTL
                     KVAEPPKRGAGIKVPDVATLVDKLRNEAKVIG"
     gene            complement(3090..4445)
                     /locus_tag="PK242_10855"
     CDS             complement(3090..4445)
                     /locus_tag="PK242_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOZ94561.1"
                     /translation="MAQDGVLTALAAGGLSGMRRLRIDDWSEWWAAVTRPVVLGEFLL
                     MLASGLLAWGVTALLRRHRALPAQAQPGILLGRRGVDGVLFPMLWLTLAWLARLVWLR
                     WEASAPLLRVALPVLLALLVIRVGVKVLQAAFPRARFVRALERSISWLVWGLWVLWLS
                     GLLPVLLEEADHLTWKVGAATLSLRSLLEGGLNVVIALLLALWLSSAIESRLLREVGG
                     GTLSMRKMLANATRAVLVFVAVLVALSAVGIDLTALSVLGGAIGVGIGLGLQRLAANY
                     VSGFVVLAERALRIGDMVRVDGFEGQITDIRARYTVIRSSSGRESVVPNELMVTQRVE
                     NLSLADRRIWQSTTVGVGYGSDARQVQQLLRDAALTVPRVLREPAPSASLSAFGADAL
                     EFTVGYWMSEPEAGVLGLRSEVNLAILDALRRHGIEIPFPQRVLHHPPPAPTPAESRV
                     E"
     gene            complement(4424..5233)
                     /locus_tag="PK242_10860"
     CDS             complement(4424..5233)
                     /locus_tag="PK242_10860"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOZ94562.1"
                     /translation="MSASPAIPAEPLVRHQRDARGVHRLTLNQPKSFNVLSEAMLAEL
                     QRLLDQIGADPAARAVVIGAEGKAFCAGHDLKQMKASPRLDYYQALFAQCSRMMLSIL
                     RLPVPVLARVQGLATAAGCQLVAQCDLAVAAQGARFGVNGIDVGLFCSTPSVPLVRNI
                     APKQAMEMLLTGGFIGAEEAQARGLVNRVVPPEQLDETVEALVGAILAKPRAAIAAGK
                     ELFYRQRELGIEAAYQLAGQTMACNMMLDVTQEGVQAFIDKREPAWRKTAC"
     gene            complement(5354..>5568)
                     /locus_tag="PK242_10865"
     CDS             complement(5354..>5568)
                     /locus_tag="PK242_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOZ94563.1"
                     /translation="FISAGFDAHREDDMGQLGLTESDFAWITERVKGVAERFAQGRIV
                     SCLEGGYNLNALARSVEAHLRVLAEV"
BASE COUNT          849 a         1943 c         1862 g          914 t
ORIGIN      
        1 tttcttcgcc gaccaggtag ccgacggcgc cgccgtggtc gccgaactgc agcaccgccg
       61 tcgggctggc cttgatgccc atcttgtgct cgatgctgac gcagtacacg tcgttgcgct
      121 cgcccaggct gccatcgggc ttgaccatga acttgggcac cacgaacagg ctgatgccct
      181 tgacgccctc gggcgcgccc tgcacgcgcg ccagcaccag gtgcacgatg ttctcggcca
      241 tgtcgtgctc gccgtaggtg atgaagatct tggtgccgaa gatgcggtag gtgccgtcgg
      301 gctgcggctc ggcgcgcgtg cgcaccgccg ccaggtcact gccggcctgg ggctcggtca
      361 ggttcatggt gccggtccag tggccggaca ccaactgctc caggtaggtc gccttcagct
      421 cctcgctgcc cgccgtgagc agcgcctcga tggcaccgtc ggtcagcagt gggcacaggg
      481 cgaagctcag gttggccgaa ttgaggactt cgccacaggc tgcgccaatg gtcttgggca
      541 ggccttgccc gccgaaggcc gccgggtgct gcaggccttg ccagccgcct tcgatgtatt
      601 ggcggtaggc ctccttgaag ccggcgctgg tggtcaccga cgtgcccgag tggctggacg
      661 gcgccttgtc ggcctcgaag ttgagcggtg ccaccacccc ttcattgaag cgcgcgcatt
      721 cttccagcac cgcctgggcg gtctccaggc cggcatcctc gaagccgggg atggcggcga
      781 tctggtcgat gcgggccaga tgctcgatgt tgaacagcag gtctttgacg ggggccttgt
      841 agctcatggc agggatcctg aaacatgcaa aacaaaaggg catcgcatgc gacgcccttg
      901 aaccgagaca tcccagtcca ccgaaacact gaaatcggaa cggtgctgtc caccagatac
      961 gggcacgctg cggcgttgcc catgctcgcc acagcaccgg ctgttgcagc gctttgcgcc
     1021 ttgcatcgcc ccccgtctgg ggtgtccctc gccgcccatt tcaatgtttc agtggaccag
     1081 cgcgcacctt cgttcaggtg ccgcgccgga caatcacagg gcctgggtca gcgcgggcac
     1141 ggcctcgaac aggtcggcca ccaggccgta gtcggccacg ctgaagatcg gcgcctcttc
     1201 gtccttgttg atggcgacga tgaccttgct gtccttcatg cccgccaggt gctggatggc
     1261 gcccgaaatg ccggcggcga tgtagagctg cggcgccacg atcttgccgg tctgccccac
     1321 ctgcaggtcg ttgggcgcat agccggcatc caccgccgcg cggctggcgc cgatggcggc
     1381 gcccagcttg tcggccagcg gggtcatgac ctcgttgaac ttctcggcgc tgcccagggc
     1441 gcgaccgccg gagacgacga tcttggcggc agtcagttcg gggcggtcgc tcttggcgat
     1501 ttcctggcca acgaagccgc tcttgccgct gtcggccacg gcagccacgc tctcgaccgc
     1561 ggcgctgccg ccgctggccg cggcggcgtc aaagccggtg gcacgcacgg tgatcacctt
     1621 ggtggcgtcg gccgactgca cggtggcgat ggcgttgccg gcgtagatcg ggcgctcgaa
     1681 ggtgtcggga ctgtcgacct tggtgacttc gctgatctgc gccacgtcca gcctggcggc
     1741 cacacgcggg gcggcgttct tgccgccggc ggtggcggga aacaggatgt ggctgtagtt
     1801 gccggccacc gccagcacct gggccgccag gttttcggcc aggccgtggg ccagggctgg
     1861 ggcgtcggcg tgcagcacct tggcgacgcc ggcgatctgg ctggcggcct gggcggcggc
     1921 agcggcgttg tggccggcca ccagcacgtg cacgtcgcca ccgcaggcgg ccgcggcggt
     1981 gacggtgttg agggtggcgc ccttgatgga ggcgttgtcg tgttcggcaa tgacgagtgc
     2041 ggtcatgaga aatctccggg aatggggtgc gatgacaaat gacggatgac ttcgccgcgc
     2101 ttcaatgccc aagggttcaa gggtcacgcc gcgccagcgg cgtcatcgaa gcggcgaagc
     2161 cgcgcggtca ttcggtcatc cgatgacttt ggcttcgttg cgcagtttgt ccaccagggt
     2221 ggcgacgtcg ggcaccttga tgccggcgcc gcgcttgggc ggctcggcca ctttcagcgt
     2281 tttcaggcgc ggcgccacat ccacgcccag gtcctcgggc ttgacggtgt cgagcggctt
     2341 tttcttggcc ttcatgatgt tcggcagcgt gacgtagcgc ggctcgttca ggcgcaggtc
     2401 ggtggtgacc accgccggca gcgtgagggc cagggtttcc aggccgccgt cgacctcgcg
     2461 cgtgacgctg gccttgccgt cggcaatctc gaccttgctg gcaaaggtgg cctgcggcag
     2521 ctcggccagc gcggccagca tctggccggt ctggttggcg tcgtcgtcga tcgcctgctt
     2581 gcccaggatc accaggccgg gctgctcctt gtccaccaaa gccttgagca gcttggccac
     2641 ggccagcggc tgcagctcgg catcggtttc gaccaggatg gcgcggtcgg cgccgatggc
     2701 catggcggtg cgcagggttt cctggcactg ggccacgccg caggagacgg ccaccacttc
     2761 ggtgagcacg cccttttcct tcaggcgcac ggcctcttcg acggcgattt cgtcaaaggg
     2821 gttcatgctc atcttgacgt tggcgatgtc cacgccggtg ccgtcgctct tgacgcgcac
     2881 cttcacgttg tagtccacca cgcgcttgac ggcgacgagt gctttcatgg gtacagatct
     2941 cctgggctgc tgttcacttg acgtttgccg gcgcggcagt ttcccgctgc gtggcgactc
     3001 gaaatcattc tagttcacga cctgtggcgg gccgcctgac ggacatcact caagcgacag
     3061 atccaccaaa atcgaacgat cgtgcttttt cattctaccc tgctttcagc gggcgtcggc
     3121 gcaggcggcg gatggtgcag cacgcgctgc gggaacggga tctcgatgcc gtggcggcgc
     3181 agcgcgtcga ggatcgccag gttgacctcg gagcgcaggc ccagcacccc ggcttcgggc
     3241 tcgctcatcc agtagccgac cgtgaactcc agcgcatcgg caccgaaggc cgacagcgag
     3301 gccgacggcg ccggctcgcg cagcacgcgg ggcaccgtca gcgccgcatc gcgcagcaac
     3361 tgctgcacct ggcgcgcatc gctgccgtaa ccgacaccga cggtggtcga ctgccagatg
     3421 cgccggtcgg ccagcgacag gttttccacc cgctgcgtca ccatcagttc gttgggcacc
     3481 accgactcgc gccccgaact gctgcgaatc acggtgtagc gcgcgcggat gtcggtgatc
     3541 tgtccttcga aaccgtcgac gcgcaccatg tcgccgatgc gcagtgcgcg ctcggccagc
     3601 accacgaagc ccgacacgta gttggccgcc agccgctgca ggcccaggcc gatgccgacg
     3661 ccgatggctc cgcccagcac cgacagcgcg gtcagatcga tgcccaccgc cgacagcgcc
     3721 accagcaccg ccacgaacac cagcaccgcg cgcgtggcgt tggcgagcat cttgcgcatc
     3781 gacagggtgc cgccgccgac ctcgcgcagc aggcgcgact cgatggccga cgacagccaa
     3841 agcgccagca gcagggcgat gacgacgttc agcccccctt ccagcaggct gcgcaggctg
     3901 agcgtggccg cgccgacctt ccaggtcagg tggtcggcct cttccagcag caccggcagc
     3961 aaaccgctca gccacagcac ccacaggccc cagaccagcc aggaaatgct gcgctccagc
     4021 gcgcgcacga agcgcgcgcg cggaaacgcc gcctgcagca ccttgacccc gacccggatc
     4081 accagcagcg ccagcagcac cggcagcgcc acgcgcagca gcggtgccga agcttcccag
     4141 cgcagccaga ccaggcgcgc cagccaggcc agggtcagcc acagcatcgg aaacagcacg
     4201 ccgtccacgc cacggcgccc caacagaatg cccggttggg cctgcgccgg cagcgcgcgg
     4261 tggcgccgca gcagcgccgt gacgccccag gccagcaggc cgctggccag catcagcagg
     4321 aactcgccca gcaccaccgg ccgcgtgacg gccgcccacc attcgctcca gtcgtcgatg
     4381 cgcagccggc gcatgccgct cagcccaccg gccgccagcg cggtcagcac gccgtcttgc
     4441 gccacgccgg ttcccgcttg tcgatgaagg cctgcacgcc ttcctgggtg acgtccagca
     4501 tcatgttgca ggccatggtc tggccggcca gctggtaagc ggcctcgatg cccagctcgc
     4561 gctggcgata gaacagctcc ttgccggcgg cgatggccgc acgcggcttg gccaggatgg
     4621 cgccgaccag cgcctccacc gtctcgtcga gctgttcggg cggcaccacg cggttgacca
     4681 ggccacgcgc ctgcgcctcc tcggcgccga tgaagccgcc agtcagcagc atctccatgg
     4741 cctgcttggg cgcgatgttg cgcaccagcg gcacgctggg cgtgctgcag aacaggccga
     4801 cgtcgatgcc gttgacaccg aagcgcgccc cctgcgccgc caccgccaga tcgcactgtg
     4861 ccaccagctg acagccggcc gccgtggcca ggccctgcac gcgtgccagc accggcaccg
     4921 gcagcctgag gatcgacagc atcatgcggc tgcactgggc aaacagcgcc tggtaatagt
     4981 ccagccgcgg actggccttc atctgcttga ggtcatggcc ggcacagaag gccttgcctt
     5041 cggcaccgat caccaccgcg cgcgcggccg ggtcggcgcc gatctggtcg agcagccgct
     5101 gcagctcggc cagcatggcc tcgctcagca cgttgaagga cttgggctgg ttcagcgtca
     5161 gccggtgcac gccgcgcgca tcgcgctgat ggcgcaccag gggctcggca ggtatggctg
     5221 gagatgcgct catggtgatg gctccgtccg gtggccggcg gcgacccggc ggcgccgtca
     5281 tgcactacaa atacgatagc tgctggcgct tgccgatcgg ccgctggaag ccgattctat
     5341 tcacatctcc ggatcacacc tcggccagca cgcgcaggtg cgcctccacg ctgcgcgcca
     5401 gcgcgttgag gttgtagccc ccttccaggc agctcacgat gcggccctgg gcaaagcgct
     5461 cggccacgcc cttgacgcgc tcggtgatcc aggcgaagtc ggattcggtc aggccgagct
     5521 ggcccatgtc gtcctcgcgg tgggcgtcga agccggcgct gatgaaca
//
LOCUS       DAOOOG010000164         5460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_17952_length_5460_cov_31.554857, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000164 DAOOOG010000000
VERSION     DAOOOG010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17952_length_5460_cov_31.554857"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="PK242_10870"
     CDS             complement(<1..1512)
                     /locus_tag="PK242_10870"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOZ94564.1"
                     /translation="MDAATTPKVANVKLLINGQFIESRTTQWRDVVNPATQQVLARVP
                     FATPDEVNAAVAAAKQAFKTWRKTPIGTRARIFLKLQQLIRDNMKELAALLTAEQGKT
                     LPDAEGDVFRGLEVVEHAAAIGNLQLGELANNVASGVDTFTVLQPLGVCAGITPFNFP
                     AMIPLWMFPMAIATGNTFVLKPSEQDPMVTMRLCELALEAGIPPGVLNVVHGGEDVVN
                     AICDHQDIKAISFVGSTKVGTHVYNRASLAGKRVQCMMGAKNHAIVMPDANREQSINA
                     ILGASFGAAGQRCMAISVVLLVGEAQKWLPEFVEKAKTLKVSAGTTPGADLGPLISCA
                     ARSRVESLIERGVAEGAKLELDGRNPSIDGFEKGNFVAPTIFSGVKPGMSIYDQEIFG
                     PVLCVVAADDLEQAIDFINANPNGNGTAIFTQSGAAARMFKEDIDVGQVGINLPVPVP
                     VPLFSFTGSRASKLGDLGPYGKQVVLFYTQTKTVTERWFDDSTLHHGVNTTISL"
     gene            1627..2517
                     /locus_tag="PK242_10875"
     CDS             1627..2517
                     /locus_tag="PK242_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ94565.1"
                     /translation="MDWDNLRFFLELARTGTLTAAARRLAVDHTTVARRLQALEKQAG
                     ATLFTREADGHRLTEAGQRLLPGAQAMERAAQQLALGAPAGGDGPAGVVRLGATEGFG
                     TALLAPRLAWLTRQHPGLTLDLLALPRLLHLSRREADIVISLERPRHETVVVTRLSDY
                     ALHLYGQREYLARRPLIQRREDLRHHAFVSYVDDLLFTKELQFLGELHQPARFALRST
                     SISAQLEAVRAGAGLAVLPAFLADQDPTLARVLPAEGRFIRTFWMSMPGEIRHQPAIQ
                     TVWGFLRQAVAEMQPRLLPP"
     gene            complement(2572..3414)
                     /locus_tag="PK242_10880"
     CDS             complement(2572..3414)
                     /locus_tag="PK242_10880"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOZ94566.1"
                     /translation="MSHAELIIRACRQIEAAEAPPSLAALAQAAGLSPHHFQRLFKAH
                     TGVTPRAYAEGQRARRLRARLTQAASVTEAIYDAGFNAPSRFYENARELIGMTPSRWR
                     AGGAGETIRFALGQCSLGAILVAESDQGICSIALGDAPEPLLAQLQAQFPRADLVGGD
                     AAFERRVAQVVGFVEQPALGLELPLDVRGSAFQQRVWQALRAVPAGQTLSYAELARRI
                     GQPRAARAVARACASNVLAVAIPCHRVVRGDGALAGYRWGVARKQALLESEGWHEPAL
                     NNDA"
     gene            complement(3603..4073)
                     /locus_tag="PK242_10885"
     CDS             complement(3603..4073)
                     /locus_tag="PK242_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOZ94567.1"
                     /translation="MEAWLQSLLAALALPEFGLSSVFVVAFISATLLPVGSEPAVFGL
                     VQLNPALLWPAIGVATVGNTLGGVVSWWMGLGAHHVADRYRHSPTHLRALDWLQRIGP
                     RACFLSFLPIIGDPLCAVAGWLRLPLLPCTVWMAAGKLLRYTGMTLGLLYLWPK"
     gene            4204..5244
                     /gene="dusB"
                     /locus_tag="PK242_10890"
     CDS             4204..5244
                     /gene="dusB"
                     /locus_tag="PK242_10890"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOZ94568.1"
                     /translation="MRIGPYTLPNALFVAPMAGVTDRPFRQLCRAFGAGYAVSEMVTS
                     RRDLWHTLKTSRRADHVGEPGPIAVQIAGTDAPMMAEAARYNIERGAQIIDINMGCPA
                     KKVCNQWAGSALMQDEALALQIIEAVVAACAPHGVPVTLKMRTGWCAAHKNAPALARA
                     AESAGVQMVTVHGRTREQGYRGEAEYDTIAAIKAALTIPVVANGDIDSPAKARAVLAA
                     TGADALMIGRAAQGRPWIFREIAHFLATGRELAPPLVLEVRRALLAHLQDHYGLYGEC
                     TGVRSARKHIGWYVRGLPGGEAFRQRMNSVESAALQWQLVADYFEELADTHERLPAQP
                     LRPQPAPGVQAH"
     gene            5248..>5460
                     /locus_tag="PK242_10895"
     CDS             5248..>5460
                     /locus_tag="PK242_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ94569.1"
                     /translation="MSKKHIDECLRENLEAYFRDLRGTEPGGLYDMMLRAMEKPLLDV
                     VMQHAGHNQSRAAEWLGMNRNTLRKKL"
BASE COUNT          846 a         1872 c         1924 g          818 t
ORIGIN      
        1 caggctgatg gtggtgttca cgccatggtg cagcgtgctg tcgtcgaacc agcgctcggt
       61 gacggtcttg gtctgggtgt agaacagcac cacctgcttg ccgtaggggc ccaggtcgcc
      121 cagcttggaa gcgcggctgc cggtgaacga gaacagcggc acgggcaccg gcaccggcag
      181 gttgatgccg acctggccga cgtcgatgtc ttccttgaac atgcgcgcgg ccgcgcccga
      241 ctgcgtgaag atggcggtgc cgttgccgtt cgggttggcg ttgatgaagt cgatggcctg
      301 ctccaggtcg tcggcggcga ccacgcacag cacggggccg aagatctcct ggtcgtagat
      361 gctcatgccg ggcttgacgc cgctgaagat ggtcggggcg acgaagttgc ctttctcgaa
      421 gccgtcgatg ctggggttgc ggccgtcgag ttccagcttg gcgccttcgg ccacgccgcg
      481 ctcgatcagg ctttcgacgc gcgagcgcgc ggcgcaggag atcagcgggc ccaggtcggc
      541 accgggcgtg gtgccggccg agaccttgag cgtcttggcc ttctcgacga actcgggcag
      601 ccatttctgc gcttcaccca ccagcagcac taccgagatc gccatgcagc gctggccggc
      661 ggcgccgaac gacgcgccca ggatggcgtt gatcgactgc tcgcggttgg cgtcgggcat
      721 gacgatggcg tggttcttgg cgcccatcat gcattgcacg cgcttgccgg ccaggctggc
      781 gcggttgtaa acatgggtgc ccaccttggt cgaaccgacg aagctgatgg ccttgatgtc
      841 ctggtggtcg caaatggcgt tgaccacgtc ctcgccgcca tgcaccacgt tcagcacgcc
      901 gggcggaatg ccggcttcca gcgccagctc gcacaggcgc atggtgacca tggggtcctg
      961 ctcggagggc ttgagcacga aagtgttgcc ggtggcaatg gccatgggga acatccacag
     1021 cggaatcatg gccgggaagt tgaacggcgt gatgccggcg cacacgccca gcggctgcag
     1081 cacggtgaag gtgtccaccc cgctggcgac gttgttggcc agctcgccca gctgcaggtt
     1141 gccgatggcg gcggcgtgct ccaccacctc caggccgcgg aacacgtcgc cctcggcgtc
     1201 gggcagggtc ttgccctgtt cggccgtcag cagcgcggcc agctccttca tgttgtcgcg
     1261 gatcagctgc tgcaatttca ggaagatgcg cgcacgggtg ccgatggggg tcttgcgcca
     1321 ggtcttgaag gcctgcttgg cggcggcgac ggcggcgttg acctcatcgg gcgtggcaaa
     1381 cggcacgcgc gccagcacct gctgggtggc cgggttgacc acgtcgcgcc actgggtggt
     1441 gcgggattcg atgaactgac cgttgatcag cagcttgacg ttggcgactt tgggggtggt
     1501 ggcggcgtcc atgggtgtct cctggggtgg gatggaatgg acgcatggta gctgtgcgaa
     1561 tttgccatga caatgcgcag gcacgcactc ttgatctgca aaaatgcaca acgcggcgag
     1621 gtcggcatgg actgggacaa cctgcgcttc tttctggaac tggcgcgcac cggcacgctg
     1681 acggccgccg cgcgccgcct ggcggtggac cacaccacgg tggcgcggcg cctgcaggcg
     1741 ctggaaaagc aggccggcgc caccctgttc acgcgcgagg ccgacggcca ccgcctgacc
     1801 gaggccggcc agcgcctgct gcccggcgcc caagccatgg agcgcgccgc ccagcaactg
     1861 gcgctgggcg caccggccgg cggcgacggc ccggccggcg tggtgcggct gggcgccacc
     1921 gagggcttcg gcaccgccct gctggcgccg cggctggcct ggctgacgcg ccagcacccg
     1981 gggctgacgc tggacctgct ggcgctgccg cggctgctgc acctgtcgcg gcgcgaggcc
     2041 gacatcgtca tctcgctgga gcgcccgcgc cacgagacgg tggtggtcac ccgcctgagc
     2101 gactacgccc tgcacctgta cggccagcgc gagtatctgg cgcgccggcc gctgatccag
     2161 cggcgcgagg acctgcgcca ccacgccttc gtcagctacg tcgacgacct gctgttcacc
     2221 aaggagctgc agtttctggg cgagctgcac cagccggcgc gttttgcgct gcgcagcacc
     2281 agcatcagcg cgcagttgga agccgtgcgt gccggcgccg gcctggccgt gctgccggcc
     2341 tttctggccg accaggaccc gacgctggcg cgcgtgctgc cggccgaagg gcgcttcatc
     2401 cgcaccttct ggatgagcat gccgggcgaa atccgccacc agccggcgat ccagaccgtg
     2461 tggggctttc tgcgccaggc ggtggccgaa atgcagcccc gcctgctgcc gccatagtgt
     2521 ctgaaacgct attgaaataa gagctgctgg cgcaagctgc gaggtcatcc gtcatgcgtc
     2581 attgttcaac gcaggttcgt gccagccctc actttcgagc agcgcctgct tgcgcgccac
     2641 gccccagcga tagccggcca gcgcgccgtc gccgcgcacc acgcggtggc aagggatggc
     2701 cacggccagc acgttgctgg cgcaggcgcg cgccacggcg cgggcggcgc gcggctggcc
     2761 gatgcggcgc gccagctcgg cgtagctcag cgtctggccg gccggcaccg cgcgcagcgc
     2821 ctgccacacg cgctgctgga aggcgctgcc gcgcacgtcc agcggcagct ccagccccag
     2881 ggccggctgc tcgacgaaac ccaccacctg cgccacgcgg cgctcgaagg cggcgtcgcc
     2941 acccaccagg tcggcgcgcg gaaactgcgc ttgcagctgc gccagcagcg gctcgggtgc
     3001 gtcgcccagg gcaatgctgc agatgccctg gtcgctttcg gccaccagga ttgcgcccag
     3061 cgaacactgg ccgagcgcga agcggatcgt ctcgcccgcg ccgccggcgc gccagcggct
     3121 gggcgtcatg ccgatcagct cgcgtgcgtt ttcgtagaag cggctgggcg cattgaagcc
     3181 ggcgtcgtag atggcctcgg tcacgctggc cgcctgggtc agccgggcgc gcagccggcg
     3241 tgcgcgctgg ccctcggcat aggcgcgcgg tgtcaccccg gtgtgggcct tgaacaggcg
     3301 ctggaagtgg tgcggcgaca gccccgccgc ctgcgccagc gcggccagcg aaggcggcgc
     3361 ctcggccgcc tcgatctggc ggcaggcgcg gatgatcagc tcggcatggc tcatggcgcc
     3421 actgtacggc gcgcccacgg ggccggccat ccgtttcttg cggccatgtc cccgctcatg
     3481 tcctgcaggg atgcggcctg cccgaagatg acacgcggat cacggccttt tctaggatcg
     3541 aatccggcgt cggtgcctgc ctggcaagcg ccagaagcta tttaattgat agcatatcca
     3601 ggttacttcg gccacaggta gagcagcccc agggtcatgc cggtgtagcg cagcagcttg
     3661 ccagccgcca tccagacggt gcagggcaac agcggcagac gcaaccagcc ggccacggcg
     3721 cacagcgggt cgccgatgat gggcagaaac gacagaaagc aggcgcgcgg gccgatgcgc
     3781 tgcaaccagt ccagcgcccg caggtgggtg ggcgaatggc gatagcggtc ggccacgtga
     3841 tgggcgccca gtcccatcca ccagctcacc acgccgccca gcgtgttgcc gacggtggcc
     3901 accccgatgg ccggccacag cagtgcgggg ttgagctgta ccaggccgaa cacggccggc
     3961 tcggagccga ccggcaacaa ggtcgccgag atgaaggcga ccacgaacac gctggacagc
     4021 ccgaactcgg gcagcgccag cgcggccagc agcgattgca accaggcttc catcaaggcg
     4081 cgagtgtagg gatcgcggct ggcggcgcgg cgtttcgatt gctgaaattt tgggcagtta
     4141 gaatccgctt ccctgtcgcg cacccccggc tcatcgaggc cgacgccgct gcgccccgtg
     4201 cccatgcgaa tcggtcccta cacccttccc aacgccctgt tcgtggcccc catggcgggg
     4261 gtgacggacc ggccgtttcg ccagctgtgc cgcgccttcg gggccggcta cgcggtcagc
     4321 gagatggtga cctcgcggcg cgatctgtgg cacacgctca agacttcgcg ccgcgccgac
     4381 cacgtcggcg agcccggccc gatcgcggtg cagatcgccg gcaccgacgc gcccatgatg
     4441 gccgaggcgg cgcgctacaa catcgagcgc ggcgcccaga tcatcgacat caacatgggc
     4501 tgcccggcca agaaggtgtg caaccaatgg gccggcagcg cgctgatgca ggacgaggcg
     4561 ctggcgctgc agatcatcga ggccgtggtg gccgcctgcg cgccgcacgg cgtgccggtg
     4621 acgctcaaga tgcgcaccgg ctggtgcgcc gcccacaaga acgccccggc gctggcgcgc
     4681 gccgccgaaa gcgccggcgt gcagatggtc acggtgcatg gccgcacgcg cgagcagggc
     4741 tatcgtggtg aagccgaata cgacaccatc gccgccatca aggccgcgct gacgatcccg
     4801 gtggtggcca atggcgacat cgactcgccc gccaaggcgc gcgccgtgct ggccgccacc
     4861 ggggccgacg cgctgatgat cggccgcgcc gcccagggcc ggccctggat ctttcgcgag
     4921 atcgcgcact ttctggccac cgggcgcgag ctggcgccac cgctggtgct ggaggtgcgg
     4981 cgcgcgctgc tggcgcacct gcaggaccac tacggcctgt acggcgaatg caccggcgtg
     5041 cgctcggcgc gcaagcacat cggctggtat gtgcgcggcc tgcccggtgg cgaagcgttt
     5101 cgccagcgca tgaacagcgt cgagagtgcc gccctgcaat ggcagttggt ggccgactat
     5161 tttgaagaac tggccgacac gcacgagcgc ctgcccgctc agcccctgcg gccccagcct
     5221 gcacccgggg tgcaggccca ctgaaccatg agcaagaaac acatcgacga gtgcctgcgc
     5281 gagaacctgg aggcctactt ccgcgacctg cgcggcaccg agccgggggg gctgtacgac
     5341 atgatgctgc gcgcgatgga aaagccgctg ctcgacgtcg tcatgcagca cgccggccac
     5401 aaccagagcc gcgccgccga atggctgggc atgaaccgca acaccctgcg caagaagctg
//
LOCUS       DAOOOG010000165         5448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_18008_length_5448_cov_31.787502, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000165 DAOOOG010000000
VERSION     DAOOOG010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18008_length_5448_cov_31.787502"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PK242_10900"
     CDS             complement(<1..544)
                     /locus_tag="PK242_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ94570.1"
                     /translation="MPPPRLELTQIRKRYPGVLANDDVSLTVQPGEIHAVLGENGAGK
                     STLMKIIYGAVKPDAGELRFDGQPVRIANPQQARRLGIAMVFQHFSLFETLSVAENVW
                     LGLDKALPLAEVTRRIDQTAAAYGLEIDPARPVHTLSVGEMQRVEIIRALLTEPRLLI
                     LDEPTSVLTPQAVDKLFVTLR"
     gene            complement(639..1979)
                     /gene="miaB"
                     /locus_tag="PK242_10905"
     CDS             complement(639..1979)
                     /gene="miaB"
                     /locus_tag="PK242_10905"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOZ94571.1"
                     /translation="MSRKVFIKTFGCQMNEYDSAKMADVLGAAQGYEPTDDPEQADLI
                     LFNTCSVREKAQEKVFSDLGRVKHLKARGTLIGVGGCVASQEGEAIIRRAPYVDVVFG
                     PQTLHRLPELLAERQKQNAPQVDIRFPEIEKFDHLPPARVDGASAFVSIMEGCSKYCS
                     YCVVPYTRGEEFSRPLDDVLTEIAGLADQGVKEVTLLGQNVNAYRGPMGDSGEIADFA
                     LLIEYVADIPGIERIRYTTSHPNEFTPRLIEVYGKVPKLVSHLHLPVQHGSDRILMAM
                     KRGYTAMEYKSIVRKLRAVRPDLSLSSDFIVGFPGETEQDFDKLMKLIEDVGYDASFS
                     FIFSPRPGTPAANLHDDTPHAVKLARLQHLQAVVEQNVRAISASRVGTRQRILVEGPS
                     RRNPNELMGRTECNRIVNFDAGAQGARLVGQMIEVDITEALPHSLRARLASAAG"
     gene            complement(2068..2340)
                     /locus_tag="PK242_10910"
     CDS             complement(2068..2340)
                     /locus_tag="PK242_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94572.1"
                     /translation="MKFIELPDVLLNGKPERLAHAVRVRDERNAIVMPGVVNGRTAVV
                     NYLRDARGQVREVWILSPQEAALPLKPVPTTRRPAPSADATASYTH"
     gene            complement(2553..3482)
                     /gene="cysK"
                     /locus_tag="PK242_10915"
     CDS             complement(2553..3482)
                     /gene="cysK"
                     /locus_tag="PK242_10915"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOZ94573.1"
                     /translation="MPADNVLQTIGRTPHVRIRRLFGPEARVWVKCERANPGGSIKDR
                     IALAMIEAAEQSGQLQPGSTIIEPTSGNTGVGLAMVAAVKGYRLVLVMPDSMSVERRR
                     LMLAYGASFDLTPRDQGMKGAIARAEELAAQLRAAGQGAWIPQQFDNPANAAVHEQTT
                     AQEILADFPDGLDALITGVGTGGHLTGCARVLKARWPQLKVFAVEPDQSPVISGGAPG
                     PHPIQGLGAGFIPKNLDTALLDGVIQVGAEAARDYGRRCAREEGLLVGISSGAALAAI
                     AQKLPELPAGSRILGFNYDTGERYLSVDGYLPT"
     gene            3617..3781
                     /locus_tag="PK242_10920"
     CDS             3617..3781
                     /locus_tag="PK242_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94574.1"
                     /translation="MTLFDSTMLMLGVSCLMMLIGFATRESRWGLGLMVLGTAGALLL
                     IAYTILAMLE"
     gene            3831..4149
                     /gene="rnpB"
                     /locus_tag="PK242_10925"
     ncRNA           3831..4149
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK242_10925"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4481..>5448)
                     /locus_tag="PK242_10930"
     CDS             complement(4481..>5448)
                     /locus_tag="PK242_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ94575.1"
                     /translation="SCPRAGWLAGKEARYMEHEMPFGELALVTPDGQLLHALDWQSEQ
                     PVPLLKNVQRLTAPNPGFMTGPGTNSYLVGDPRSGYIAIDPGPADPAHVERLWRAAGG
                     HIRAIVCTHSHPDHSPGALPLQALCQPPPPILGLPSLPTARAHSQFQPDRALQDGERL
                     ALTGQAPDGEITTHTLRAIHTPGHAANHVCLLLEEDGLLFSGDHILNGSTTVIDPPDG
                     DMTAYLESLDKLDAACAEAQAHFILPAHGHVLGQARDTIAHLKAHRLAREAKVLAVMK
                     ADPHGTLDDWVAKAYDDVPERVWPVARRSLLAHVERLRAMAPGND"
BASE COUNT          758 a         1808 c         1993 g          889 t
ORIGIN      
        1 ggcgcagggt gacgaacagc ttgtccaccg cctgcggcgt cagcaccgag gtgggttcgt
       61 ccaggatcag caggcgcggc tcggtcagca gggcgcggat gatctccacg cgctgcatct
      121 cgcccacgct cagcgtgtgc accgggcgcg ccgggtcgat ctccagcccg taggcggcgg
      181 cggtctggtc gatgcggcgc gtcacctcgg ccagcggcag cgccttgtcc agacccagcc
      241 acacgttctc ggccacgctc agggtctcga acaggctgaa atgctggaac accatggcga
      301 tgcccagccg gcgcgcctgc tgcggattgg cgatgcgcac cggctgaccg tcaaagcgca
      361 gctcgccggc gtcgggcttg acggcgccgt agatgatctt catcagcgtg ctcttgccgg
      421 cgccgttctc gcccagcacg gcgtggattt cgcccggctg caccgtcagc gacacgtcgt
      481 cgttggccag cacgcccggg tagcgcttgc ggatctgcgt cagttcgagg cggggcggcg
      541 gcatcggggc gggcgggcgc ggtgtcggcg ggtggtgcgg tggcgggcac cctcaggcgc
      601 cgacgcgcaa tgtaacaccc gccggccggc gctgccgctc agcctgcggc gctcgccagg
      661 cgcgcgcgca gcgagtgcgg cagggcttcg gtgatgtcga cctcgatcat ctggccgacc
      721 aggcgcgcgc cctgtgcgcc ggcgtcgaag ttgacgatgc ggttgcactc ggtgcgaccc
      781 atcagctcgt tcgggttgcg ccgcgacggc ccttcgacca ggatgcgctg gcgcgtgccg
      841 acgcggctgg cgctgatggc gcgcacgttc tgctccacca ccgcctgcag gtgctgcagg
      901 cgcgccagct tgacggcgtg cggtgtgtcg tcgtgcaggt tggccgccgg cgtgccgggg
      961 cgcgggctga agatgaagct gaacgaggcg tcgtagccca cgtcctcgat cagcttcatc
     1021 agcttgtcga agtcctgctc ggtctcgccc ggaaagccga cgatgaagtc gctcgacagg
     1081 ctgaggtccg gccgcaccgc gcgcagcttg cggacgatgc tcttgtattc catggcggtg
     1141 tagccgcgct tcatggccat caggatgcgg tcgctgccgt gctgcaccgg caggtgcagg
     1201 tggctgacca gcttgggcac cttgccgtac acctcgatca ggcgcggggt gaattcgttg
     1261 gggtggctgg tggtgtagcg gatgcgctcg atgccgggga tgtcggccac gtattcgatc
     1321 agcagggcga agtcggcgat ctcgccgctg tcacccatcg gcccgcggta ggcattgacg
     1381 ttctggccca gcagggtgac ttccttgacg ccctggtcgg ccaggccggc gatctcggtc
     1441 agcacgtcgt cgagcgggcg gctgaactcc tcgccgcgcg tgtagggcac cacgcagtag
     1501 ctgcagtact tggagcagcc ctccatgatc gagacgaagg cgctggcgcc atcgacgcgc
     1561 gccggcggca ggtggtcgaa cttctcgatc tcggggaagc ggatgtccac ctgcggcgcg
     1621 ttctgcttct ggcgctcggc cagcagctcg ggcaggcggt gcagggtctg cgggccgaac
     1681 accacgtcca cgtagggcgc gcgccggatg atggcttcgc cctcctggct ggccacgcag
     1741 ccgccgacgc cgatcagcgt gcccctggcc ttcaggtgct tgacgcggcc gaggtcggaa
     1801 aacaccttct cctgcgcctt ctcgcgcacc gagcaggtgt tgaacaggat caggtcggcc
     1861 tgctccgggt cgtcggtggg ctcgtaaccc tgggcggcgc ccagcacgtc ggccatcttg
     1921 gccgagtcgt actcattcat ctggcagccg aaggtcttga tgaaaacttt gcgggacatg
     1981 cggtgtctcc gggaagaggg ggcgagggcg ctggccgccg ggccgcggcc gacgacgagg
     2041 cggcgcgtgg acgccgccgc gtcagggtca atgggtgtag gacgcggtgg cgtcggcgct
     2101 gggcgccggc cgccgcgtgg tcggcaccgg cttgagcggc agcgccgctt cctgcggcga
     2161 caggatccac acctcgcgca cctggccgcg ggcatcgcgc aggtagttga cgacggcggt
     2221 gcgaccgttg accacacccg gcatcacgat ggcgttgcgc tcgtcgcgca cgcgcaccgc
     2281 gtgcgccagc cgctcgggct tgccgttgag cagcacatcg ggcagctcga tgaacttcat
     2341 ctcggcccgc tcggtcttga gcggaaagcc ccgttccgcc cggacttcgg catgaaaacc
     2401 gagtccagcg cttaccaggc aagcgctggc agctataaaa aacgtagtgc ggagaacgtt
     2461 cgagcagcgg ttcatggtgt ctgtccagag gctgtggcgg cgcggcgcgc cggcgggcat
     2521 tctatgccgg tcgggtcggt ggcgccgccg cctcaggttg gcagatagcc gtccaccgac
     2581 aggtagcgtt caccggtgtc gtagttgaag cccaggatgc ggctgccggc cggcagctcg
     2641 ggcagcttct gtgcgatggc cgccagcgcg gcgccgctgc tgatgcccac cagcagccct
     2701 tcctcgcgcg cgcagcggcg gccgtagtcg cgcgcggctt cggcgccgac ctggatcacg
     2761 ccgtccagca gcgcggtgtc caggtttttc gggatgaagc cggcgcccag gccctggatc
     2821 gggtgcgggc cgggcgcgcc gccgctgatc acgggcgact ggtcgggctc gacggcaaac
     2881 accttcagct gcggccagcg cgccttcagc acgcgcgcgc agcccgtcag gtggccgccg
     2941 gtgccgacgc cggtgatcag cgcgtccagc ccgtcgggaa agtcggccag gatttcctgc
     3001 gccgtggtct gttcgtgcac cgccgcgttg gccgggttgt cgaactgctg cgggatccag
     3061 gccccctggc cggcggcgcg cagctgcgcg gccagctcct cggcgcgggc gatggcgccc
     3121 ttcatgccct ggtcgcgcgg cgtcaggtcg aagctggcac cgtaggccag catcagccgg
     3181 cgccgctcga cgctcatgct gtcgggcatc accagcacca ggcggtagcc cttgacggcc
     3241 gccaccatcg ccaggccgac gccggtgttg ccgctggtgg gctcgatgat ggtgctgccg
     3301 ggctgcagct ggccgctttg ctcggccgcc tcgatcatgg ccagggcgat gcggtccttg
     3361 atcgagccgc ccgggttggc gcgctcgcac ttgacccaca cccgggcctc ggggccgaac
     3421 aggcggcgga tgcgcacgtg cggcgtgcgg ccgatggttt gcaggacgtt gtcggcgggc
     3481 atggtgtatc tctccggtcg gtgatgggca ggggcaccta ttgtgcgagc cttgatccca
     3541 cctggcgtga tatcgatatg ccccgccgcg cggcaggtca caatggctca ctttccggct
     3601 gtcaccacgt gcccccatga ccctgttcga ctccaccatg ctgatgctgg gcgtcagctg
     3661 cctgatgatg ctgatcggct ttgccacgcg cgaatcgcgc tggggcctgg gcctgatggt
     3721 gctgggcacg gccggcgcgc tgctgctgat cgcctacacc atcctggcca tgctggagtg
     3781 aacggcggcc gcgtgcagcc ggcgcccgct gggcggataa tcacggccgt gaagcccgcc
     3841 agaccgccgc tggcgcaagg cccgaaaggg cgcgctggag gaacgtccgg actgcacagg
     3901 gcagcgcagc aggtaacacc tgtccaccgt gaggtgagga tcagagcaac agagacgagc
     3961 cgcttgagtg cgggtgaaac gggcaatctc tgcgcgcagc aacaccaagt aggccagcgt
     4021 tgacgtgacc cgcggagctg gcgggtaggt ggcaccgagc cgcacgggcg accggcggcc
     4081 cagattgatg gcggtcacgg cgcgggcaac cgcgccgcac agaatccggc gtatcggtgg
     4141 gcttcacaca ttttttgcgc ccggcaggcg gacatccgga cgcggaggac gcgaaagcac
     4201 gccaagcgcg cgaaacagcc cgatgcctgt ttcgcgccct tcgtgtccgc ccgttggtgt
     4261 cctggcgccg cgccgcgggt gggtgcgtgg cgtgctcacc ccacaggtga aggcccttga
     4321 cgcgcatggg cgcggcctgc ccgacgttga caagcggtca tctgccgtgg ccatggatcc
     4381 ggccccatga aatcgtcacc gttcgccctg agcatgacga agacatcgac aagctcggcc
     4441 cgaaccgaat gtgagacttc aaagggccgg atcaataggc tcagtcgttg cccggcgcca
     4501 tggcccggag gcgctccaca tgcgccagca gcgaacgcct ggccaccggc cagacgcgct
     4561 cgggcacgtc gtcgtaggcc ttggccaccc agtcgtccag cgtgccgtgg gggtcggcct
     4621 tcatcaccgc cagcaccttg gcttcgcgcg ccagccggtg cgccttaaga tgggcgatgg
     4681 tgtcgcgcgc ctggcccagc acgtggccgt gggccggcag gatgaagtgc gcctgggcct
     4741 cggcacaggc ggcgtcgagc ttgtccagcg attccaggta ggccgtcatg tccccatccg
     4801 gcgggtcgat gacggtggtg ctgccgttca ggatgtggtc gccgctgaac agcaggccgt
     4861 cttcttccag cagcaggcag acatggttgg cggcgtggcc aggggtgtgg atcgcgcgca
     4921 gcgtatgggt ggtgatttcg ccgtccgggg cttgcccggt cagcgccagg cgctctccat
     4981 cctgtagcgc gcggtcgggc tggaactggc tgtgggcgcg cgccgtgggc agcgaaggca
     5041 gtcccaggat gggcggtggc ggctggcaca gggcctgcag gggcagggcg ccgggtgagt
     5101 ggtcggggtg cgagtgggtg cagacgatgg cgcggatgtg cccgccggcg gcacgccaca
     5161 gccgctccac atgggccggg tcggccgggc cgggatcgat ggcgatgtag ccgctgcgcg
     5221 ggtcgcccac caggtagctg ttggtgcccg ggccggtcat gaagccgggg ttgggcgccg
     5281 tcaggcgctg cacgttcttg agcagcggca ccggctgctc ggactgccag tcgagcgcat
     5341 gcagcagctg gccatcgggc gtcaccagcg ccagttcgcc aaagggcatc tcgtgctcca
     5401 tgtagcgcgc ttccttgccg gccagccagc cggcgcgcgg gcagctgg
//
LOCUS       DAOOOG010000166         5374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_18330_length_5374_cov_22.734349, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000166 DAOOOG010000000
VERSION     DAOOOG010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18330_length_5374_cov_22.734349"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PK242_10935"
     CDS             complement(<1..544)
                     /locus_tag="PK242_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOZ94576.1"
                     /translation="MAHKGFFNAFHDVWLLDGVRTPMVDYCGALGHVSPTDLGIKAAR
                     AALQRAGVPGSDIGSVVCGNMAPGDFDQFFLPRHIGLYAGVPVEVPAIMAQRICGTGF
                     ELFRQAGEHVQGGACEAALVVGTENMTRNPIAAFEHRTGFRLGAPVGFKDYMWEALKD
                     PAPGINMIQTAENLAKKYGIT"
     gene            complement(559..2460)
                     /locus_tag="PK242_10940"
     CDS             complement(559..2460)
                     /locus_tag="PK242_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HOZ94577.1"
                     /translation="MSIPRAAAAAAPRYRPLRFGVTQARVRGGAGGVQYLNAEQALQP
                     FAERMSDRLRHWAQATPEATFLAQRVRAADGQLGDWRRISYAQALDGARRIGQALLAR
                     GLSAERPVLILSENSLEHALLALGCMWAGVPWCPASPAYSLISKDYGKLRHIVGTLTP
                     GLIWAEDGARYGAAMAATAPPDCELVLSRHGDAPSLQGRSTTAFSDLLATEATPAVDA
                     AQAATGPDTIVKFLFTSGSTKLPKAVINTHRMWCANQQQMRQSMPVLTEEPPVLVDWL
                     PWNHTFGGNHNVGMVLYNGGTLYIDEGKPTPALVGETLRNLREIAPTLYFNVPTGFEA
                     IANAMQHDDQLRRTLLSRVKMFFYAGASLAQPVWDSLFASAEREVGERIVMASGLGMT
                     ESSPFGLFVTSPDVRAGDLGLPAPGLELKLAPEGDKLEIRYRGPNITPGYWRTPETTA
                     EAFDDEGFFCTGDAVAWIDPREPDLGLRFDGRIAEDFKLATGTFVSVGPLRGRVITGG
                     APYIQDVVITGLNRAEVGALIFPTPAVRALTPLPADAPMTDVLRHPAVLQKFHSLVVD
                     LSLTATGSANRVARLMLLAEPPSIDKGEVTDKGSINQRAVLKERDALVQALYDEAPGH
                     IVIKPDSRF"
     gene            complement(2538..3326)
                     /locus_tag="PK242_10945"
     CDS             complement(2538..3326)
                     /locus_tag="PK242_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOZ94578.1"
                     /translation="MHVTHDLSVELLGPAQHLAVVRLTRAAKRNALSDGLVAALRDAF
                     QNLPASVRAAVLTGEGEHFCAGLDLSELKERDAGAGLHHSRSWHVALDAIQYGAVPVV
                     AALHGAVVGGGLELACAAHIRVADDTTFYALPEGSRGIFVGGGGSVRIPKLIGVARMT
                     DMMLTGRVYHAADGERLGFAQYLVPAGNAFDKALELAERIATNAPLTNYALMHALPRI
                     AEQPADHGLLTEALISAIAQSAPEAKERVRDFLAGKAGTKVKKG"
     gene            3506..4471
                     /locus_tag="PK242_10950"
     CDS             3506..4471
                     /locus_tag="PK242_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOZ94579.1"
                     /translation="MQRRHLLAGLAAVTLTFGPAWANGFPSRPIRIVVPNAAGGAADI
                     TARTVGEHMARTLGQPVVVENKPGAGGVVAGQAVAGAAPDGHTLLLISSGTAVSQALF
                     KSLPYDTLKAFTPVAPLASFDLVIVSKEGGAFKSLGDLIAYARQNPGKLNIGTPNIGT
                     TQNLAAELFKSAAGLDLQVVPFKATPDVIGAIRGDQIQAGLDIVPPLLTQIQAKVLRP
                     LAVTGAKPSRVLPGVPTAQEAGVKGLNAASWNGLAVPAGTPAAVIDKLNQAVNAALRD
                     PEVVKKLQTLNLDPHPGSAQDALAMLSGDIQRWNQVIDKARIERK"
     gene            4705..>5374
                     /locus_tag="PK242_10955"
     CDS             4705..>5374
                     /locus_tag="PK242_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOZ94580.1"
                     /translation="MSQTVRIGLIGYGEVGKIFSAGLLKQPGVAGVSAWDLKFARTDT
                     AAAERQHAALAGVQAQDSAAALCAASDLVIAAVTASSTLAVAGEAARHLRPGSLYLDL
                     NSASPGTKRQCAQLIDGAGAHYVESGVMTSVPPYGIRVPMLLGGAHAAELAARLGGWG
                     MDARAVSAELGVASAIKMCRSVMIKGLEALVIESYTAARAYGVEEHVLPTLQETFPGI
                     DWSAQ"
BASE COUNT          817 a         1780 c         1947 g          830 t
ORIGIN      
        1 gggtgatgcc gtatttcttg gccaggttct cggcggtctg gatcatgttg atgccgggcg
       61 ccgggtcctt cagggcctcc cacatgtagt ccttgaagcc caccggcgcg ccgagcctga
      121 agccggtgcg gtgctcgaac gcggcaatcg ggttgcgcgt catgttctcg gtgcccacca
      181 ccagcgccgc ctcgcaggcg ccgccctgca cgtgctcgcc ggcctggcgg aacagctcga
      241 agccggtgcc gcagatgcgc tgcgccatga tggccggcac ctccaccggc acgccggcgt
      301 acaggccgat gtggcgcggc aggaagaact ggtcgaagtc gcccggcgcc atgttgccgc
      361 acaccaccga gccgatgtcg ctgccgggca cgcccgcgcg ctgcagggcc gcgcgcgccg
      421 cctttatccc taagtcggtg ggcgagacgt ggcccagcgc gccgcagtag tcgaccatcg
      481 gtgtgcgcac gccgtcgagc agccagacgt catggaaggc gttgaaaaat cccttgtggg
      541 ccatgtgggt ctcctggttc agaagcggct gtcgggcttg atcacgatgt gccccggcgc
      601 ctcgtcgtac agcgcctgca ccagcgcgtc gcgctccttc agcacggcgc gctggttgat
      661 gctgcccttg tcggtgactt cgcccttgtc gatcgaaggg ggttcggcca gcagcatcag
      721 ccgtgcgacg cggttggcgc tgccggtggc cgtcagcgac agatcgacca ccaggctgtg
      781 gaacttctgc agcaccgccg ggtggcgcag cacatcggtc atcggtgcgt cggcgggcag
      841 cggcgtcagc gcgcgcacgg ctggggttgg aaagatcagc gcaccgactt cggcccggtt
      901 caacccggtg atgaccacgt cctggatgta gggcgcaccg ccggtgatga cgcgtccgcg
      961 cagcggcccg acgctgacga aggtgccggt ggcgagcttg aagtcctcgg cgatgcgacc
     1021 gtcgaagcgc aggcccagat cgggctcgcg cgggtcgatc caggcgaccg cgtcgccggt
     1081 gcagaagaaa ccttcgtcgt cgaaggcttc ggccgtggtc tcgggtgtgc gccagtagcc
     1141 gggcgtgatg ttggggcccc gatagcgaat ttcgagtttg tcgccctcgg gcgccagctt
     1201 gagctcaagg ccgggcgcgg gaaggcccag atcgccggcg cgcacgtcgg ggctggtgac
     1261 gaacaggcca aacgggctgg attcggtcat gcccaggccg ctggccatga cgatgcgctc
     1321 gcccacctcg cgctcggcgc tggcaaacag gctgtcccac accggctgcg ccagcgaggc
     1381 gccggcataa aagaacatct tcacgcgcga cagcagggtc cggcgcagct ggtcgtcgtg
     1441 ctgcatcgca ttggcaatcg cctcgaagcc ggtgggcacg ttgaagtaca gcgtgggcgc
     1501 gatctcgcgc aggttgcgca gcgtctcgcc caccagcgcc ggcgtgggct tgccctcgtc
     1561 gatgtagagc gtgccgccgt tgtagagcac catgccgacg ttgtgattgc cgccgaaggt
     1621 gtggttccag ggcagccagt cgaccagcac cggcggctcc tcggtcagca ccggcatcga
     1681 ctggcgcatc tgctgctggt tggcgcacca catgcggtgg gtgttgatga cggccttggg
     1741 cagcttggtc gagccgctgg tgaacaggaa cttgacgatg gtgtcaggcc cggtggcggc
     1801 ctgcgcggcg tcgacggccg gcgtcgcttc ggtggccagc agatcgctga acgcggtcgt
     1861 gctgcgcccc tgcaggctgg gcgcgtcgcc gtggcgcgag agcaccagct cgcagtctgg
     1921 cggcgcggtg gcggccatgg ccgcgccgta gcgcgcgccg tcttcggccc agatcaggcc
     1981 gggggtgagc gtgccgacga tgtggcgcag cttgccgtag tccttgctga tgagcgaata
     2041 ggcgggcgag gccgggcacc agggcacgcc cgcccacatg cagcccagcg ccagcagggc
     2101 gtgctccagg ctgttttcgg acaggatcag caccggccgc tcggccgaca ggccgcgcgc
     2161 cagcagcgcc tggccgatgc ggcgcgcgcc atccagcgcc tgggcgtagc tgatgcggcg
     2221 ccagtcgcca agctggccgt cggctgcgcg cacgcgctgg gccagaaacg tggcctcggg
     2281 cgtggcctgc gcccagtgcc gcaggcggtc ggacatgcgc tcggcaaagg gctgcagcgc
     2341 ctgctcggca ttcaggtact gcacgccgcc ggcgccgccg cgcacgcgcg cctgggttac
     2401 gccaaagcgc agcggacgat agcgcggagc tgccgcagca gcggcccgag ggatgctcat
     2461 ggcggtttcc attagcttct ggtttgatag ctgctggcgc tggatgggca agggctgcag
     2521 gccgatttgg cttgaagtca gcccttcttg accttggtgc ccgccttgcc ggccagaaag
     2581 tcgcgcacgc gctctttggc ctcgggcgcc gactgggcga tggcgctgat cagcgcctcg
     2641 gtcagcaggc cgtgatcggc cggctgctcg gcgatgcgcg gcagcgcgtg catcagcgcg
     2701 tagttggtca gcggcgcgtt ggtggcgatg cgctcggcca gctccagcgc cttgtcgaag
     2761 gcgttgccgg cgggcaccag gtactgggcg aagcccagac gctcgccgtc ggcagcgtgg
     2821 tagacgcggc cggtgagcat catgtcggtc atgcgcgcca cgccgatcag cttggggatg
     2881 cgcaccgagc cgccaccgcc gacgaagatg ccgcgcgagc cttcgggcag ggcgtagaag
     2941 gtggtgtcgt cggccacgcg gatgtgcgcc gcgcaggcca gctccagccc gccgcccacc
     3001 accgcgccgt gcagcgcggc caccaccggc accgcgccgt actggatggc gtccagcgcc
     3061 acatgccagc tgcgcgaatg gtgcaggccg gcacccgcgt cgcgttcctt gagttcggac
     3121 agatccagcc cggcgcagaa gtgctcgccc tcgccggtga gcacggcggc gcgcacgctg
     3181 gccggcaggt tctgaaaggc gtcgcgcagg gccgcgacca ggccgtccga cagcgcgttg
     3241 cgcttggccg cgcgcgtcag gcgcaccacg gccaggtgtt gcgcggggcc cagcagttcg
     3301 accgagagat cgtgggtgac gtgcatcgtg aggtatctcc aacggccgtt attgtttatg
     3361 atgaataatt aaatgcaagg ctcgcgggca catctttgct aggggtaaac acgggtgccg
     3421 ggcagacgct ggcgggtgcg cgggcgtaga ctccagaccc gcccgccgcc ttgctgcgtc
     3481 caccctctgt ccggagatcc atctcatgca acgtcgacac ctgctggccg gtctggccgc
     3541 cgtcaccctg acctttggcc cggcgtgggc caacggcttt ccctccaggc cgatccgcat
     3601 cgtggtgccc aacgcggccg gcggcgcggc cgacatcacc gcgcgcacgg tgggcgagca
     3661 catggcgcgc accctgggcc agccggtggt ggtcgagaac aagcccggcg ccggcggcgt
     3721 ggtggccggg caggcggtgg cgggcgcggc gcccgatggc cacacgctgc tgctgatctc
     3781 cagcggcacg gcggtgagcc aggcgctgtt caagtcattg ccgtacgaca cgctcaaggc
     3841 cttcacgccg gtggcgccgc tggccagctt cgatctggtg atcgtgagca aggagggcgg
     3901 cgccttcaag tcgctgggcg atctgatcgc ctatgcgcgc cagaaccccg gcaagctcaa
     3961 catcggcaca cccaacatcg gcaccacgca gaatctggcg gccgagttgt tcaaaagcgc
     4021 cgccgggctg gacctgcagg tggtgccgtt caaggccacg ccggacgtga tcggcgcgat
     4081 tcgcggcgac cagatccagg ccgggctgga catcgtgccg ccgctgctga cgcagatcca
     4141 ggccaaggtg ctgcgcccgc tggccgtcac cggcgccaag ccctcgcgcg tgctgccggg
     4201 cgtgccgacc gcgcaggagg cgggtgtcaa aggcctgaac gccgcgtcct ggaacggcct
     4261 ggccgtgccg gcgggcacgc ctgcggcggt gatcgacaaa ctcaatcagg ccgtcaacgc
     4321 cgccctgcgc gaccccgaag tggtcaagaa gctgcagacg ctcaacctcg atccgcaccc
     4381 cggcagcgcg caggatgcgc tggccatgct gtcgggcgac attcagcgct ggaaccaggt
     4441 gatcgacaag gcgcgcatcg agcgcaagtg agcgctatcg agcggtcgaa cgccgaaccg
     4501 ccgaacaacc gtacgcagag ggcgcgaagg atgcgcagag gacgcgacag aacagccaag
     4561 gctctgttgt ctggctggtc ggtgcgcggg gcgtgattca cggggctggt gatgacattt
     4621 gccgtgacat tgggcgagtt tcaaattcgc ctgtagcgca acaggggcaa gcgtcattag
     4681 ctatcgaatc aggagtattt gatcatgtcg caaaccgtcc gcatcgggct cattggttat
     4741 ggggaagtcg gcaagatctt cagcgccggc ctgctgaagc agccaggggt ggcaggcgtg
     4801 agcgcctggg acctcaagtt cgcccggact gacacggcgg cggccgaacg ccagcacgcg
     4861 gcgctggcgg gtgtgcaggc gcaggactcg gcggcggcgc tgtgcgcggc aagtgatctg
     4921 gtgattgccg ccgtcacggc gtccagcacg ctggccgtgg ccggcgaggc cgcgcgccac
     4981 ctccgcccgg gcagcctgta cctggacctg aactcggcct cgcccggcac caaacggcag
     5041 tgcgcgcagc tgatcgacgg cgccggcgcg cactacgtcg agtcgggggt gatgacctcg
     5101 gtgccgccct atggcattcg cgtgcccatg ctgctgggcg gcgcgcacgc cgccgagctg
     5161 gccgcgcggc tcggcggctg gggcatggac gccagggcgg tgagcgccga gctgggcgtg
     5221 gccagcgcca tcaagatgtg ccgcagtgtg atgatcaagg gcctggaggc gctggtgatc
     5281 gaaagctaca cggcggcgcg cgcctatggg gtggaagagc acgtgctgcc cacattgcag
     5341 gaaacctttc ccggcatcga ctggagcgcg cagg
//
LOCUS       DAOOOG010000167         5305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_18655_length_5305_cov_25.429524, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000167 DAOOOG010000000
VERSION     DAOOOG010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18655_length_5305_cov_25.429524"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /gene="tyrS"
                     /locus_tag="PK242_10960"
     CDS             complement(<1..581)
                     /gene="tyrS"
                     /locus_tag="PK242_10960"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOZ94581.1"
                     /translation="MSDNELTNAAVTEGVRAAMAISLRGCDELLPQQDWLKKLARAEA
                     TGQPLRIKLGLDPTAPDIHLGHTVVLNKMRQLQDLGHQVIFLIGDFTSLIGDPSGRNS
                     TRPPLTAEQIRVNAETYREQAFKVLDASKTELRYNSEWSDALGARGMIELAARYTVAR
                     MMERNDFAERFKAGQSISVHEFLYPLMQGYDSVA"
     gene            743..2104
                     /locus_tag="PK242_10965"
     CDS             743..2104
                     /locus_tag="PK242_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOZ94582.1"
                     /translation="MKQTLISTGLALADFIHRHPKRLTAALAAVLLGGTGGAFAVASL
                     APAAPTAPLRLVSEAVQPSDLSAQAEALDQHAFTLYRSDQTRATDTPEALLKRLGLAD
                     PAAAAFLRKNETTRQALFGGRAVAGRSVTAEATDGAELQSLRTHWAESATDSHFKRLV
                     VERAGNGFSVRVETAPLVVNQRLASGTIRNALFAATDEAGLPDSVTRQLTEIFEANID
                     FHRGLRRGDRFAVVYETLEADGEPVRAGRILSAEVVNRGKTHQALWFKEPGASQGSYY
                     TPDGQSLRRAYLLSALKVSRITSGFGMRNHPVYGYSRSHAGVDYAAPTGTPVHTIGDG
                     TVEFAGVQSGYGKVVYIKHRNKKDTTVYAHLSQIGVKAGDPVSQGQVIGEVGQTGVAT
                     GPHLHLEFRVNNEPVDPTEMLAEQREHVPVSPAAKAAFARLKTDMRTQLTAAGQIALS
                     NFE"
     gene            complement(2255..2632)
                     /gene="erpA"
                     /locus_tag="PK242_10970"
     CDS             complement(2255..2632)
                     /gene="erpA"
                     /locus_tag="PK242_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HOZ94583.1"
                     /translation="MSAVAQDLQTPTAMPDPIIFTDSAAAKVAELIAEEGNPDLKLRV
                     FVQGGGCSGFQYGFTFDEVANEDDTTMTKNGVSLLIDAMSYQYLVGAEIDYREDLQGA
                     QFVIKNPNAETTCGCGSSFSTAE"
     gene            complement(2723..3178)
                     /locus_tag="PK242_10975"
     CDS             complement(2723..3178)
                     /locus_tag="PK242_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOZ94584.1"
                     /translation="MLGIKRQPSVRSVIGAGMRVQGPCAFPDGLQIDGAVLGDVVAEG
                     GQPSVLVIGEAGRVEGAVSADHVVIAGTVVGPVLARELLELQPTARVQGDVRYKTLEM
                     QQGAVIAGQLQPQLTPPPAAPEAPLGRASEPAEPTLDPEPGLPPNPDRP"
     gene            complement(3292..3684)
                     /gene="rpsI"
                     /locus_tag="PK242_10980"
     CDS             complement(3292..3684)
                     /gene="rpsI"
                     /locus_tag="PK242_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOZ94585.1"
                     /translation="MIGDWNNGTGRRKSSVARVFLKKGSGKITVNGKAIDEFFGRQTS
                     IMICKQPLVLTDNLETFDVQVNVRGGGESGQAGAVRHGITRALIDYDAGLKPQLSAAG
                     FVTRDAREVERKKVGLHSARRRKQFSKR"
     gene            complement(3697..4125)
                     /gene="rplM"
                     /locus_tag="PK242_10985"
     CDS             complement(3697..4125)
                     /gene="rplM"
                     /locus_tag="PK242_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOZ94586.1"
                     /translation="MKTFSAKPADVTHEWFVIDATDKVLGRVASEVALRLRGKHKAIY
                     TPHVDTGDYIVVVNAAKLRVTGAKEQDKMYYRHTGYPGGIRETNFRDLQAKHPGRALE
                     KAVKGMLPKGPLGYAMIKKLKVYGGAEHPHSAQQPKVLEV"
     gene            4317..5279
                     /gene="rlmJ"
                     /locus_tag="PK242_10990"
     CDS             4317..5279
                     /gene="rlmJ"
                     /locus_tag="PK242_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HOZ94587.1"
                     /translation="MFSYRHAFHAGNHADVLKHLTLVATLRHLMRKDSPLTMIDTHAG
                     AGIYRLDAEAAQTSGEALAGIARLAPPNAGDSAPNSATARSRQAPDAIVSEYDALTDY
                     LALLAGFNRTGASGGPWRVYPGSPLLMHALMTEPARAAVHDRLRLFELHPTDAQALQA
                     HIAQLDAGRQVGMARQDGFAALKALLPPPASGSGSRRALVLIDPSYEIKSDYAKVAGA
                     VQDALRRFATGVYLVWYPIIPRPEAHTLPRRLRTLAQQAERAWLQATLAVGAAPGSNG
                     LSASGMFVINPPHTLAAALRSALPRVQQALARGPQASWSVDTGD"
BASE COUNT          801 a         1888 c         1741 g          875 t
ORIGIN      
        1 gccaccgagt cgtagccctg catcagcggg tagaggaact cgtgcacgct gatcgactgg
       61 ccggccttga agcgctcggc aaagtcgttg cgctccatca tgcgcgccac ggtgtaccgg
      121 gcggccagct cgatcatgcc gcgcgcgccc agggcgtccg accattcgct gttgtagcgc
      181 agctcggtct tgctggcgtc cagcaccttg aaggcctgct cgcggtaggt ttcggcgttc
      241 acgcggatct gctcggccgt gagcggcggg cgcgtgctgt tgcgcccgga cgggtcgccg
      301 atcaggctgg tgaagtcgcc gatcagaaag atcacctgat gccccaggtc ctgcagctgg
      361 cgcatcttgt tcagcaccac ggtgtgaccc aggtggatgt cgggcgccgt cgggtccagc
      421 cccagcttga tgcgcagcgg ctggccggtg gcttcggcgc gggccagctt cttcagccag
      481 tcctgctgcg gcagcaattc gtcgcatccg cgcagcgaaa tggccatggc ggcgcgcaca
      541 ccctcggtga cggcggcgtt tgtaagttcg ttgtcagaca tcgtacggtc aggttgtagg
      601 ggtcggccgg gctcgttata ctgacccgtt accgccccga ttttaggggt gcgctcggtt
      661 gccgagcctt cttcgtggca gtcgcacacc gcccggtgcg tgcctgtcct gtcctgaact
      721 ccctgtcccg gaacagccgc gcgtgaagca aaccctgatt tccaccggcc tggccctcgc
      781 cgatttcatt catcgccacc ccaaacgcct caccgccgcc ctggccgccg tgctgctggg
      841 cggtaccggg ggcgcctttg ccgtcgcctc tctggcgccc gccgcgccga ccgcgccgct
      901 gcgcctggtc tcggaggcgg tgcaacccag cgacctgagc gcccaggccg aggcgctgga
      961 ccagcacgcc ttcaccctct accgcagcga ccagacgcgc gccaccgaca cccccgaggc
     1021 cctgctcaag cgcctgggcc tggccgatcc ggccgccgcg gcctttctgc gcaagaacga
     1081 aaccacccgc caggcgttgt tcggcgggcg cgcggtggcc ggccgcagtg tgacggccga
     1141 agccacggac ggcgccgaat tgcagtccct gcgcacccat tgggcagaaa gcgccaccga
     1201 cagccatttc aagcgcctgg tggtcgagcg tgccggcaac ggcttcagcg tgcgggtcga
     1261 aaccgccccg ctggtggtca accagcgcct ggccagcggc accatccgca acgccctgtt
     1321 cgccgccacc gatgaggccg ggctgcccga cagcgtgacg cgccagctga ccgaaatctt
     1381 cgaagccaac atcgacttcc accgcggcct gcgccgcggc gaccgctttg ccgtggtcta
     1441 cgaaacgctg gaagccgacg gcgagccggt ccgtgccggg cgcatcctga gcgccgaggt
     1501 ggtcaaccgc ggcaagacgc accaggcgct gtggttcaag gagccgggcg ccagccaggg
     1561 cagctactac acccccgacg gccagagcct gcgccgcgcc tacctgctgt cggcgctcaa
     1621 ggtgtcgcgc atcaccagcg gcttcggcat gcgcaaccac ccggtgtacg gctacagccg
     1681 cagccatgcg ggcgtcgact acgccgcccc caccggcacc ccggtgcaca ccatcggtga
     1741 cggcacggtc gaattcgccg gcgtgcaaag cggctacggc aaggtggtct acatcaagca
     1801 ccgcaacaag aaggacacca cggtctacgc ccacctgtcg cagatcggcg tcaaggcggg
     1861 cgacccggtg agccaggggc aggtgatcgg cgaagtcggt cagaccggcg tcgccaccgg
     1921 cccgcacctg cacctggaat tccgcgtcaa caacgagccg gtggacccga ccgagatgct
     1981 ggccgaacag cgcgagcatg tgcccgtctc gcccgccgcc aaggccgcct tcgcacgcct
     2041 gaaaaccgac atgcgcaccc agctgacggc ggccggccag atcgccctca gcaacttcga
     2101 gtgagcggcc ggcggtccgg cacgccgcag aacgctatga aatagtgagc tattgccgct
     2161 tgatcggcga agtttttgag cggttatgac cagaaaaggg cgcccatggc gcccttttct
     2221 tcgatgctgg tctgacgatg cgccggcggc cggctcactc cgccgtcgaa aacgacgagc
     2281 cgcagccgca ggtggtctcg gcgttcgggt tcttgatgac gaactgcgcc ccctgcaggt
     2341 cttcccggta gtcgatctcg gcgccgacca ggtactggta actcatggcg tcgatcagca
     2401 gcgacacgcc gttcttggtc atggtcgtgt cgtcctcgtt ggcgacctcg tcgaacgtga
     2461 agccgtactg aaagcccgaa caaccgccgc cctgcacgaa cacgcgcagt ttcagatcgg
     2521 ggttgccttc ttcggcaatc agctcggcca ccttggcggc cgcgctgtcg gtgaagatga
     2581 tgggatcggg catggccgtg ggggtctgaa ggtcttgggc aacggcactc atggtgggct
     2641 cctgcgtcgg gcccgcaccg gcgggccaga aaaatagtat atcgaatcag tcggaattag
     2701 gcaggccagg gcgcatcgtc cctcaggggc ggtcggggtt gggtggcagg cctggttccg
     2761 ggtccagcgt cggctcggcc ggttcgctgg cgcgccccag gggtgcctcg ggcgccgccg
     2821 gcggcggtgt gagctggggc tgcagctgcc cggcaatcac cgcgccctgc tgcatctcca
     2881 gtgtcttgta gcgcacatcg ccctgcacgc gcgccgtcgg ctgcagctcc agcagctcgc
     2941 gcgccagcac cggcccgacc accgtgccgg caatcaccac gtggtcggcg ctgacggcgc
     3001 cttccacgcg gccggcttcg ccgatcacca gcacgctggg ctgaccgccc tcggccacca
     3061 cgtcgcccag caccgcaccg tcgatctgca ggccgtccgg aaatgcgcaa ggcccctgca
     3121 cccgcatgcc ggcgccgatg acgctgcgca cggatggctg ccgcttgatg cccaacatgt
     3181 ctgctctccc gccaccgcga tggcttgcac gcccttttta ccacgcggcc cacaaaaaag
     3241 ccgcaccgcc cggaaggcgg cgcggccttt gcgccagggc gccaagcccg atcagcgctt
     3301 gctgaactgc ttgcgccggc gagcggagtg caggccgacc ttcttgcgct cgacctcgcg
     3361 cgcgtcgcgc gtcacgaaac cggcggccga cagctgcggc ttcaggcccg cgtcgtagtc
     3421 gatcagggcg cgcgtgatgc cgtggcgcac ggcaccggcc tggcccgatt caccgccgcc
     3481 gcgcacgttg acctggacgt cgaaggtctc caggttgtcg gtcagcacca gcggctgctt
     3541 gcagatcatg atcgaggtct gacggccgaa gaactcgtca atggccttgc cgttgaccgt
     3601 gatcttgccg gagccttttt tcagaaacac gcgggcgacg ctggacttgc gacggccggt
     3661 gccattgttc caatcaccaa tcatctcgtc ggctccttag acttcaagca ctttgggctg
     3721 ctgggcgctg tgcggatgct cggcgccacc gtagaccttc agcttcttga tcatggcgta
     3781 acccagcggg cccttgggca gcatgccctt gaccgccttt tccagcgcgc ggccggggtg
     3841 cttggcctgc aggtcgcgga agttggtttc gcggatgccg cccgggtagc cggtgtggcg
     3901 gtaatacatc ttgtcctgct ccttggcgcc agtgacgcgg agcttggcgg cgttgacgac
     3961 cacgatgtag tcgccggtgt cgacgtgagg cgtgtaaatg gctttgtgct tgccgcgcag
     4021 acggagggcc acttcgctgg cgacccgtcc gagcaccttg tcggtggcgt caatcacaaa
     4081 ccactcgtgc gtcacgtcag cgggttttgc gctgaacgtt ttcatgagtt ttccttgtgg
     4141 agggtttgcc tggccctttt ccacggtcgg cgctcctctt gctgcgggag cctcttaggt
     4201 gggatgcttg cttttgccgc ggggccagat cgctgcaaag cccgccattt tagcggcgtt
     4261 tggggcaaag gcgcaaggct ggcgcccgac gcgccgaggg ccgctaccat cgggccatgt
     4321 tcagctatcg ccacgccttt catgccggca accacgccga cgtgctcaag cacctgacgc
     4381 tggtggccac gctgcgccac ctgatgcgca aggacagccc gctgaccatg atcgacacgc
     4441 acgcgggcgc cggcatctac cggctcgacg ccgaagcggc gcagacctcg ggcgaggcgc
     4501 tggcgggcat cgcgcgcctg gcgcccccga acgccggcga ttcagcaccg aattcggcta
     4561 cagcgcgctc cagacaagcg ccagacgcta tcgtttctga gtacgatgcg ctgaccgact
     4621 acctggccct gctggccggc ttcaaccgca cgggcgcctc cggcggcccc tggcgggtgt
     4681 atcccggctc gccgctgctg atgcatgccc tgatgacgga gccggcgcgc gccgcggtgc
     4741 acgaccgcct gcgcctgttc gagctgcacc ccaccgacgc ccaggcgctg caggcgcaca
     4801 tcgcccagct cgacgcgggg cggcaggtcg gcatggcgcg gcaggacggc tttgcggcgc
     4861 tgaaggcgct gctgccgccg ccggccagcg gcagcggctc gcgccgtgcc ctggtgctga
     4921 tcgaccccag ctacgagatc aagagcgact acgccaaggt ggccggcgcg gttcaggacg
     4981 ccctgcggcg ctttgccacc ggcgtctatc tggtgtggta cccgatcatc ccgcgccccg
     5041 aggcccatac gctgccgcgc cgcctgcgca ccctggcgca gcaggccgag cgcgcctggc
     5101 tgcaggccac cctcgccgtc ggcgcagcac ccggcagcaa cggactgtcg gccagcggga
     5161 tgttcgtcat caatccgccg cacaccctgg ccgcggccct tcggtcggcc ctgccccggg
     5221 tccaacaggc cctcgcccgc ggcccccagg ccagctggag cgtggacacc ggcgactgag
     5281 ccgctggcgc cgcggccatg gcgcg
//
LOCUS       DAOOOG010000168         5248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_18909_length_5248_cov_22.262469, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000168 DAOOOG010000000
VERSION     DAOOOG010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18909_length_5248_cov_22.262469"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PK242_10995"
     CDS             complement(<1..246)
                     /locus_tag="PK242_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOZ94588.1"
                     /translation="MRTFYSTSQGLALPPGHRFPMGKYAALRDRLSAECPGLRLAQAP
                     PASDAELALAHAPAYVAAVVQGRLDARAQREIGFPWSE"
     gene            428..973
                     /locus_tag="PK242_11000"
     CDS             428..973
                     /locus_tag="PK242_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOZ94589.1"
                     /translation="MLTPEQLVASQKANMETLYGLTTKAFEGVEKLVELNLQATKAAL
                     AESQAHANAMLSVKDAQELIALQAGLFQPLAEKAAAYSRHLYDIASGTGAEFSKAFES
                     KVAEGQAQFASLVDNAAKNAPAGSETAVAMMKSAVAAASNAFESVQKAVKQASDVAEA
                     NFNAVATSATNVAKTATAKKR"
     gene            1429..2034
                     /locus_tag="PK242_11005"
     CDS             1429..2034
                     /locus_tag="PK242_11005"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HOZ94590.1"
                     /translation="MNYQGNISSSDFPEITVGESYTLTLVFDNGGTSANSQTWGAAHL
                     TCAIWRMNNARNVVYAQNLVATPPSTVTGSATTNGAGGLTGMFSSVSSIPGDPGYPAG
                     SFTSSGISLTAPVHWFANSANGVFYDALGGTQNREFSDAAGGVIMNPANWSAPTPFSG
                     SCSAAPSAVGVAPVPTLGEWGLGLLGLAAAGLGAQRLRRRR"
     gene            2194..3243
                     /locus_tag="PK242_11010"
     CDS             2194..3243
                     /locus_tag="PK242_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOZ94591.1"
                     /translation="MIQRLTLLATAALLCAGSAHAQDNVLNLYSARHYSTDEALYNDF
                     TKATGIKINRVDADDAGIIARLKAEGSASPADVILLVDAARLARGEQDGLFLPIKSKL
                     LEDTIPASLRAQPTADGATPWFGFSTRARVIVYDKMKVKPTEVDSYEKLADPQNKGKL
                     CIRSGSHPYNLSLFGAVLEHMGPQKTEAWLKGLVDNMARAPKGGDTDQIKAVAAGECQ
                     IAVTNSYYLARLMRSSKPEDRTVAERVGVVFPNQSSWGTHVNIAGAAVARHSKHVDSA
                     TRFLEYLASPSAQNYFANGNNEWPVAKGLVTDNPALKAMTGGSGQFKSETIPVGAVGA
                     NQVKVQQMLDRVGFK"
     gene            complement(3337..4440)
                     /locus_tag="PK242_11015"
     CDS             complement(3337..4440)
                     /locus_tag="PK242_11015"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOZ94592.1"
                     /translation="MLWSLASGAPAQNPPELQAQTVASGLQHPWAVAFLPGGRFLVTE
                     RPGRLRVVDADGRVQPPLHGLPDIAAGGQGGLLDVITDAAFERNRTIYFCFSQPGPGA
                     TNGTALARARLSDDLTGLQELRVIFSQQPKVASTLHFGCRIVERRVDGRPDGTLWLTL
                     GERFSRRDDAQRLDNHLGKIVRVGKDGHVPADNPLVGRPGALPEIWSHGHRNVQGAAL
                     APDGALWTHEHGPMGGDEINRPLPGGNHGWPRVSFGLHYDGSPVGDGASGGAGLVAPL
                     HHWTPSIAPSGMVFLSSDRYGPAWRGNLFVGALKHRQLLRIELKDGQVVAQHPLLQAL
                     GQRIRDVRQGPDGWLYVLTDSPSGQLLRLVEKR"
     gene            4928..>5248
                     /locus_tag="PK242_11020"
     CDS             4928..>5248
                     /locus_tag="PK242_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ94593.1"
                     /translation="MKRPQLVLVGNGMAGVRTLEELLKLAPDLYDITVFGAEPHPNYN
                     RILLSPVLAGEQTLEQIVLNDWSWYEENGITLHTGCTVTEVNRARRMVHATRADGETI
                     SVPYD"
BASE COUNT          917 a         1830 c         1625 g          876 t
ORIGIN      
        1 ctcgctccac ggaaagccga tctcgcgctg cgcccgcgca tccagccggc cctgcaccac
       61 cgcggccacg taggccggcg catgggccag cgccagttcg gcgtcgctgg ccggcggcgc
      121 ctgcgccagc cgcagcccgg ggcattcggc gctcagccga tcgcgcaggg cggcgtactt
      181 gcccatcgga aagcgatgcc cgggcggcag cgccaggccc tgcgaggtcg agtagaaggt
      241 cctcatgcca cgccgccgcc aacggcgccc gcgccaccgg ttagaccgcc gcatctaaaa
      301 atgctgcaac gcaccaaaaa cgcttgcatt cctgcccccg ggccctaaaa ttccatccca
      361 tgctgcaccg caacataaca agcagcaacc cgtttttttt tgatccacca ttgtctagga
      421 gaaccctatg ctgacccccg aacaactcgt cgcttcccag aaggccaaca tggaaaccct
      481 gtacggcctg accaccaagg ccttcgaagg cgttgaaaag ctggtcgagc tgaacctgca
      541 agccaccaag gccgccctgg ccgagtcgca ggcccacgcc aacgccatgc tgtcggtcaa
      601 ggacgcccag gagctgatcg ccctgcaggc tggtctgttc cagccgctgg ccgagaaagc
      661 cgccgcctac agccgtcacc tgtacgacat cgcttcgggc accggcgccg agttcagcaa
      721 ggcgttcgag tccaaagtgg ccgaaggcca ggcccagttc gcctcgctgg tggacaacgc
      781 cgccaagaac gcccccgccg gctccgagac cgccgtcgcc atgatgaaga gcgccgtggc
      841 tgccgccagc aacgcgttcg agtcggtgca gaaggccgtc aagcaggctt ccgacgtcgc
      901 tgaagccaac ttcaacgccg tggccaccag cgccaccaac gtcgccaaga ccgcgaccgc
      961 caagaagcgc tgatcgccga ccggccagcc cgcccacggt ttcacccgac ccgaggcgca
     1021 ctggccaccc ggtgctttgc cgcccgccct gcgtggtcgg caaagccacc agagccaaac
     1081 cggtgcccgg cgcgggtttg gctctggtgc cgagtccggc acctcatgct tgtctcctcg
     1141 gatcctttcg acacccatcc ggttcaggga tcccttcaag ggctgatcgc aagatcagcc
     1201 ctttttttgt cgcgttaatg cgacacgtat gccgcatgtt ggagacgcaa gtcacgctgg
     1261 agcattttgt catgttacgc tgtaagtcaa tgtttcggtg ttcttggctc tttgtattga
     1321 ggttcgatca acatgtgcac ttcacgcctg cccagccgct ctcttcgtgc cctgatcatt
     1381 gcggctgtcg cggccgcgtg ttcagtcgca gttgccgcgc cgtatgccgt gaactatcaa
     1441 ggcaacatca gcagctccga ctttccagaa atcacggtcg gtgaaagcta tacgctgacg
     1501 ctggtgttcg acaacggcgg cacgtctgcc aattcgcaaa cttggggcgc cgcccacctc
     1561 acatgcgcca tctggcgtat gaacaatgcg cgtaacgtgg tgtacgcaca gaacttggtc
     1621 gccacaccac cgtctacggt caccggcagc gcaacgacca atggtgcagg gggactgacg
     1681 ggtatgtttt ccagtgtcag ctccatacct ggcgacccag gctatccagc gggctccttc
     1741 acgtctagtg gaatttcatt gactgcaccg gttcactggt ttgccaacag tgcgaatggt
     1801 gttttctatg acgctctcgg tggcactcag aaccgagaat tcagtgatgc tgctggcggg
     1861 gttatcatga acccggcaaa ctggagtgcg ccaacgccgt tttccggcag ctgcagtgct
     1921 gcaccttctg ccgttggtgt agcacctgta cctacgcttg gtgaatgggg attgggtctt
     1981 ttgggcttgg ccgctgcggg attgggtgcg caacgcctgc gccgccgccg ctgaatctcc
     2041 cacgtcgcaa tctgaggccg ccttagggcg gcttttcttt tgccatagcc accgccccac
     2101 cgcggcccga ccgccctcgt aacgagaaca attctcgttt aatatatcaa ctgctccctc
     2161 ttttttgttc atgtctgcaa gaaggacctc tcaatgattc aacgcctcac cctcctggcc
     2221 accgccgccc ttctgtgcgc gggttccgcg cacgcccagg acaatgtcct gaacctgtac
     2281 tccgcccgcc actactcgac cgatgaggcg ctgtacaacg acttcaccaa ggccaccggc
     2341 atcaagatca accgcgtgga cgccgacgac gccggcatca tcgcgcgcct gaaggccgag
     2401 ggcagcgcct cgccggccga cgtgatcctg ctggtggacg ctgcccgcct ggcgcgcggc
     2461 gagcaggacg gcctgttcct gccgatcaag tccaagctgc tggaagacac cattcccgcc
     2521 agcctgcgcg cccagccgac cgccgacggc gccacgccct ggttcggttt ttccacccgc
     2581 gcccgcgtca tcgtgtacga caagatgaag gtcaaaccga ccgaggtgga cagctacgaa
     2641 aagctggccg atccgcagaa caagggcaag ctgtgcatcc gcagcggttc gcacccctac
     2701 aacctgagcc tgttcggtgc cgtgctggaa cacatgggcc cgcagaagac cgaggcctgg
     2761 ctcaagggcc tggtcgacaa catggcgcgc gcgcccaagg gcggcgacac cgaccagatc
     2821 aaggccgtgg cggccggcga atgccagatc gccgtgacca acagctacta cctggcgcgc
     2881 ctgatgcgct cgagcaagcc cgaggaccgc acggtggccg agcgcgtcgg cgtggtgttc
     2941 cccaaccagt cgagctgggg cacgcatgtc aacatcgccg gcgcggcggt ggcgcgccat
     3001 tccaagcatg tggacagcgc caccaggttc ctggaatacc tggccagccc gtcggcgcag
     3061 aactactttg ccaacggcaa caacgagtgg ccggtggcca agggcctggt caccgacaac
     3121 cccgccctca aggccatgac cggcggcagc ggccagttca agagcgagac cattccggta
     3181 ggcgccgtgg gcgccaacca ggtcaaggta cagcagatgc tggaccgcgt cggcttcaag
     3241 tgaagcggcg cttcgccctg gcttgccaag cgccgcccag cggcgctttt ttcacgctga
     3301 gtcgagctgc agcgattgcc tggcaagcgc caacagctat cgtttttcaa ccaagcggag
     3361 cagctgcccg gacgggctgt cggtcagcac gtagagccag ccgtcggggc cctgcctgac
     3421 gtcgcggatg cgctggccca gcgcctgcag cagcgggtgc tgcgccacca cctggccgtc
     3481 cttcagttcg atgcgcagca gctgccgatg cttgagcgcg ccgacgaaca ggttgccgcg
     3541 ccaggccggc ccgtagcgat cgctgctcag aaacaccatg cccgaaggcg cgatcgaggg
     3601 cgtccagtgg tgcagcgggg ccaccaggcc ggcaccgccg ctggcgccat cgcccaccgg
     3661 cgagccgtcg tagtgcaagc caaagctcac ccgcggccag ccgtggttgc cgcctggtag
     3721 cgggcggttg atttcgtcgc ctcccatcgg gccatgctcg tgcgtccaca gcgcgccgtc
     3781 gggtgccagc gcggcgccct ggacgttgcg gtggccatgg ctccagatct cgggcaaggc
     3841 gcccgggcgg ccgaccagcg ggttgtccgc gggcacgtgg ccgtccttgc ccacacgcac
     3901 gatcttgccc aagtggttgt ccagccgctg ggcgtcgtcg cggcgcgaga agcgctcgcc
     3961 cagcgtcagc cacagcgtgc cgtcgggccg cccatcgacc cgccgctcga cgatgcggca
     4021 gccgaaatgc agtgtgctgg cgaccttggg ttgttggctg aagatcacac gcagctcctg
     4081 caggcccgtc aggtcgtcgc tcagccgcgc acgcgccagc gccgtgccgt tggtcgcgcc
     4141 cggcccgggc tgggagaagc agaaatagat cgtgcggttg cgctcgaacg ccgcatcggt
     4201 gatcacgtcc agcaggccgc cctgccctcc ggcggcgatg tcgggcaggc catgcagcgg
     4261 tggctgcacc cggccgtcgg cgtcgaccac gcgcaggcgc cccggccgct cggtcaccag
     4321 aaatcgtcct ccaggcagaa aggccacggc ccaagggtgc tgcaagccgc tggccacggt
     4381 ctgcgcctgc agctcgggtg ggttttgcgc cggcgctccc gatgccagcg accacagcat
     4441 gccggccagc gtgatccgga cccagggctt tggcaagacg ctcatcgtga cgctccattc
     4501 tcccgtgcgg gcgtgccgtg ccaagtgcca ccgcaccgcc ccggccgggg cctccagcgt
     4561 agcgcagagc gggtgttgtg gcttgctcgg ccacgcgctg gcgggccggg ccgaccgagc
     4621 ctgagcgcta agtcccggcg gacgcaccac gaaggctgtc agaactggtg cgccctgcgc
     4681 gtcgcgctgc accattttgt ggcgcaggcc ccaggacgat gcagcggccc tttgtgtccc
     4741 agggggcaga ccgtcgggtc gcagctggtc tggccccatg gccacgccgc tgcgcgggga
     4801 acctcgatct ggcgcggatc ttgcttgaag caggtcaggc ctgcatttcc gggggccgtg
     4861 ccacacgcct cggcgttcgg tcggcacccg ggaagcagtc cctgaccttg tcacaggatt
     4921 tgctgcaatg aagagacccc aactcgtgct ggtaggcaat ggcatggcgg gcgtgcgcac
     4981 gctggaggag ctgctcaagc tcgcgcccga tctctatgac atcacggtgt tcggcgccga
     5041 gccacacccc aactacaacc gcatcctgct ctcgcccgtg ctggccggcg agcaaaccct
     5101 cgagcagatc gtgctcaacg actggtcctg gtatgaggag aacggcatca ccctgcacac
     5161 cggctgcacg gtgacggaag tcaaccgcgc ccggcgcatg gtgcatgcga cgagggccga
     5221 tggcgagacc atcagcgtgc cctacgac
//
LOCUS       DAOOOG010000169         5229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_18999_length_5229_cov_28.619830, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000169 DAOOOG010000000
VERSION     DAOOOG010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18999_length_5229_cov_28.619830"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1034)
                     /locus_tag="PK242_11025"
     CDS             complement(57..1034)
                     /locus_tag="PK242_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ94594.1"
                     /translation="MNKRQLIRSAGALTCLVLTGGAASAQDFPPRKPVTLVVGFAAGG
                     AADGAARIIAKKLGENIGQTVVVDNKGGAGGNIAHQFTAKGPADGSLLLLGSIGPLTI
                     APHLMKVGYEPFKDLAPVSGGVNFPNVLVVHKGVGVKTLAEFVALARKPGARVEFAST
                     GAGSASHLAGEMFNQQAQIDMVHVPYKGGAPALQDLLGERISAYFAAPPTAMPHVEAG
                     KLVPLATTGLKRPSYLPNIPTVAESGYPGFEALNWYAFVAPGKTPAPILDRWNTEIVK
                     VLNDAEVKKALEAHGLTPQPTTRAELTDFMKKEDVKWSAIVKQRHIQAN"
     gene            complement(1075..2250)
                     /gene="tcuB"
                     /locus_tag="PK242_11030"
     CDS             complement(1075..2250)
                     /gene="tcuB"
                     /locus_tag="PK242_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HOZ94595.1"
                     /translation="MVSLEALAREARALANGEVVPMTGAETEVARQLTICNACRYCEG
                     FCAVFPAMTRRLEFGKADVHYLANLCHNCGACLHACQYAPPHEFMVNVPQAMATVRGQ
                     TYADYAWPPVLGRLYKSNGLTLSVALALGLALFLLLAVLTKGSIGEVGVGGDFYAIFP
                     HNLLVWLFAPVFLFAVLALGMGVRGLLRQVTPATGGAAAGGAAAAEAAHDALRLKYLD
                     GGHGDGCHNVDDAHTLSRRRLHHFTFYGFLLCFAATSVGTLYHYLLGWPAPYELPSLP
                     KLLGAAGGVSLVIGTVGLWRLNRTRHPLHGDPAQKPMDLGFIALLFLVAASGLLLWWA
                     RGTVAMALLLCLHLGAVMALFATLPYGKFAHGVFRSAALLRYAVEKRQPNPIGLGSE"
     gene            complement(2237..3646)
                     /gene="tcuA"
                     /locus_tag="PK242_11035"
     CDS             complement(2237..3646)
                     /gene="tcuA"
                     /locus_tag="PK242_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HOZ94596.1"
                     /translation="MVDVLVIGGGNAALCAALMAREAGASVLLLEAAPREWRGGNSGH
                     TRNLRCMHDAPQDVLVDAYPEEEYWQDLLKVTAGKTDEHLARLVIRHSSSCRDWMRRH
                     GVHFQPPLSGALHVARTNAFFMGGGKALVNAYYRSAAELGVRIRYQAPVDRLEIEHGR
                     FVAAHVQGERITARACVLAAGGFESNRDWLRQAWGRNERGEWPADNFLIRGTAYNQGV
                     LLRHMLDDHGADRIGDPTQAHMVAIDARAPLYDGGICTRIDCVSLGVVVNRDAERFYD
                     EGEDFWPKRYAIWGRLVAQQPGQIAWSIIDAKAIGRFMPPVFPGVVAATLPELARRLQ
                     LDEAAFVRTLDSYNAACVPGRFDHTALDDCRTDGLTPPKTHWARPIDTPPFYAYAVRP
                     GVTFTYLGLRTDDTAAVRFAERPSDNLFVAGEMMAGNVLGQGYTAGVGMSIGTAFGRI
                     AGVNAARAAINKGAFHGLA"
     gene            complement(3724..4650)
                     /locus_tag="PK242_11040"
     CDS             complement(3724..4650)
                     /locus_tag="PK242_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOZ94597.1"
                     /translation="MELRQLRYFVRVCEAGSMGKAALDLGMATSALSQQISRLEGELA
                     TRLLQRHATGVLPTDAGLAFLQQAQLTLRHAEDAVHAAQTARLSGRVSIGLASTTASV
                     LGVPLMHAMGQRYPAVRVHLVEALSGHLTTMLNARQLDLAILFRADGARRWSVVPLLD
                     EKLYVIGTPELPGMPAGPRVRLSQLRDLPLILPSPSHGLRALVDAAFARQRIAPCVHL
                     EVDGLSLLMDAVRAGLGATIQPGAAAARLAAGEVRRVLVADAQTGRRNLLASLSDDEL
                     SPAALAARVVLGDVARALVREGRWVGATLHKN"
     gene            complement(4729..>5229)
                     /locus_tag="PK242_11045"
     CDS             complement(4729..>5229)
                     /locus_tag="PK242_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HOZ94598.1"
                     /translation="PEHIVIDAGHSDGVRLRDYGRFGLPDAQAGDARSLLIECGFHGD
                     LASRAVAQDMCRRLLQAAGTLEPAALDATLPGWRLPDAPRQWALTVDGAVVARSPRFA
                     FTGDFTGLERFAHAGTVIGHHDGPGQGEPVVTPYDDCVLVMPSVRQAREGVTVVRFAR
                     RAPLME"
BASE COUNT          836 a         1826 c         1819 g          748 t
ORIGIN      
        1 gggccgctgc ggtcgatttg cagccggcga tttccaagtc cgacagcctc ctaggctcag
       61 ttggcctgga tgtggcgctg cttgacgatg gcgctccact tgacgtcctc cttcttcatg
      121 aagtccgtca gctcggcccg cgtggtgggt tgcggtgtca gcccatgggc ctccagcgcc
      181 ttcttcacct cggcgtcgtt cagcaccttg acgatctcgg tgttccagcg gtcgaggatc
      241 ggtgccgggg tcttgcccgg cgcgacgaag gcataccagt tcaacgcctc gaaaccggga
      301 tacccggatt cggccaccgt cgggatgttc ggcagatagg acgggcgctt gaggccggtg
      361 gtggcgagcg gcaccagctt gccggcttcg acatgcggca tggccgtggg tggcgcggcg
      421 aagtaggcgc tgatgcgctc gcccagcaga tcctgcaacg ccggcgcgcc acccttgtac
      481 ggcacgtgca ccatgtcgat ctgggcctgc tggttgaaca tctcgccagc caggtgcgag
      541 gcagagccgg cgccggtcga cgcgaactcg accctggcgc cgggcttgcg cgccagcgcg
      601 acgaattcgg ccagggtctt gacgccgacg cccttgtgca ccaccagcac gttggggaag
      661 ttgacaccgc ccgacactgg cgccagatcc ttgaatggct catagccgac cttcatcagg
      721 tgcggcgcga tggtgagcgg gccgatcgag ccgagcagca gcaggctgcc gtctgccggg
      781 cccttggccg tgaactggtg ggcaatgttg ccgccggcgc cgcccttgtt gtcgaccacc
      841 accgtctggc cgatgttctc gcccagcttc ttggcgatga tgcgtgccgc cccatcggcc
      901 gcaccgccgg cggcaaagcc caccaccagg gtcaccggct tcctgggtgg aaagtcctgc
      961 gcgctggccg cgcccccggt caggaccagg caggtcagcg caccggcact gcggatcagt
     1021 tgtcgcttgt tcatcgtgga tctccttcga acggtggata tcgagcgccc gcgctcactc
     1081 ggagccgagg ccaatgggat tgggttgccg cttctcgacc gcatagcgca gcagggccgc
     1141 gctgcggaac acgccgtgag cgaacttgcc gtagggcaag gtggcgaaca gcgccatcac
     1201 cgcccccagg tgcaggcaca gcagcagcgc catggcgacc gtgccgcgcg cccaccacag
     1261 cagcaggccg ctggcagcca ccaggaacag caacgcgatg aagcccaggt ccatcggctt
     1321 ctgcgccggg tcgccatgca gcgggtggcg cgtccgattc aggcgccaca ggccgacggt
     1381 gccgatcacc aggctgacgc cgccggctgc gcccagcagc ttgggcaggc tcggcagctc
     1441 atagggcgcc ggccagccga gcagatagtg gtagagcgtg cccacactgg tggcggcaaa
     1501 gcacagcagg aagccgtaga aggtgaagtg gtgcaggcgc cgccggctca gcgtatgggc
     1561 atcgtcgacg ttgtggcagc cgtcaccgtg accgccgtcg aggtacttca ggcgcagcgc
     1621 atcgtgcgcg gcctcggccg ccgccgcacc gccggccgcc gcgccgccgg tggcgggcgt
     1681 gacctgccgc agaaggccac gcacccccat gcccagcgcc agcacggcga acaggaacac
     1741 cggcgcaaac aaccacacca gcaggttgtg cgggaagatg gcgtagaagt cgccgcccac
     1801 gccgacttcc ccgatcgacc ccttggtcag cacggccagc agaaggaaca gcgccaaccc
     1861 cagcgccagc gccaccgaca gcgtgaggcc attggacttg tacaggcggc ccagcaccgg
     1921 cggccaggcg tagtcggcgt aggtctggcc acgcaccgtg gccatggcct gcggcacatt
     1981 gaccatgaac tcgtgtggcg gcgcgtactg gcacgcgtgc aggcaggcac cgcagttgtg
     2041 acacaggttg gcgaggtagt ggacgtcggc cttgccgaac tcgaggcgac gcgtcatggc
     2101 cggaaacacg gcacagaagc cttcgcagta gcgacaggcg ttgcagatgg tgagctggcg
     2161 tgccacctcg gtttcggcac ccgtcatcgg caccacctcg ccgttggcca gggcacgcgc
     2221 ctcgcgcgcg agggcttcaa gcgagaccat ggaaagctcc tttattgata gctgcccgcg
     2281 ccgcattgac gcccgcaatc cgcccaaacg ccgtaccaat cgacatgccg acgcccgccg
     2341 tgtagccttg tcccagcacg ttgccggcca tcatctcgcc ggcaacgaac aggttgtcgc
     2401 tgggccgctc ggcaaagcgt acggcggccg tgtcgtcggt gcgcaggccg aggtaggtga
     2461 aggtcacgcc cggccgtacg gcgtaggcgt agaacggcgg ggtgtcgatc gggcgtgccc
     2521 agtgcgtctt gggtggggtc aggccgtcgg tgcggcagtc gtcgagcgcc gtgtgatcga
     2581 agcggccagg cacacaggcc gcgttgtagc tgtccagcgt ccgcacgaac gccgcttcat
     2641 cgagctgcag cctgcgggcc agctcgggca gggtcgccgc caccacgccc ggaaacaccg
     2701 gcggcatgaa gcggccgatg gccttggcgt cgatgatcga ccaggcgatc tgccccggct
     2761 gctgcgccac caggcgcccc cagatggcgt agcgcttggg ccagaagtcc tcgccctcgt
     2821 catagaagcg ctcggcgtcg cggttgacca ccacgcccag gctgacgcag tcgatgcgcg
     2881 tgcagatgcc accgtcatac agcggcgcgc gggcgtcgat ggccaccatg tgcgcctggg
     2941 tgggatcacc gatgcggtcg gccccgtggt cgtccagcat gtgccgcagc agcacgccct
     3001 ggttgtaggc cgtgccgcga atcaggaagt tgtccgccgg ccattcgccc cgctcgttgc
     3061 gcccccaggc ctggcgcagc cagtcgcggt tggattcgaa gccgccagcg gccagcacgc
     3121 aggccctggc ggtgatgcgc tcgccctgga catgcgcggc gacgaagcgc ccgtgctcga
     3181 tctccaggcg atcgaccggc gcctgatagc gaatccgcac gccaagctcg gcggcgctgc
     3241 ggtagtaggc gttgaccagc gccttgccgc cgcccatgaa gaaggcgttg gtgcgcgcca
     3301 cgtgcagggc gcccgacagc ggcggctgaa agtgcacgcc gtgacgacgc atccagtcac
     3361 ggcagctgga ggaatggcgg atcaccaggc gcgccaggtg ctcgtcggtc ttgccggcgg
     3421 tcaccttcag caggtcctgc cagtactcct cttcgggata ggcgtccacc agcacatcct
     3481 gaggcgcatc gtgcatgcag cgcaggttgc gcgtgtggcc ggagttaccg ccgcgccact
     3541 cgcgcggcgc ggcctccagc agcagcacgc tggcgccagc ctcgcgcgcc atcagcgcgg
     3601 cacacaaggc ggcgttgccg ccaccgatga cgagtacgtc gaccaaactt gcctcctggg
     3661 ggaatggtgc gcactgtagg cacgccccat cccgtcggca agaccgccgc gcgcaacggg
     3721 tgttcagttc ttgtgaaggg tggcgccgac ccagcggcct tcgcgcacca gggcgcgcgc
     3781 cacgtcgccc agcaccacgc gcgcggccag cgcagcgggc gacagctcat cgtcggacag
     3841 gctggccagc aggttgcgcc gcccggtctg ggcgtcggcg accagcaccc tgcgcacctc
     3901 gccggccgcc aggcgggcag cggcagcgcc gggctggatg gtggcgccca ggccggcgcg
     3961 cacggcatcc atcagcagcg acaagccatc cacttccagg tgcacgcacg gcgcgatgcg
     4021 ctggcgcgca aaggccgcgt cgaccagcgc ccgcaggcca tggctgggcg acggcaggat
     4081 gagcggcaga tcgcgcagct ggctgagccg cacgcgcggg cccgccggca tgcccggcag
     4141 ctcgggcgtg ccgatcacat agagcttctc gtcgagcagc ggcaccacgc tccagcgccg
     4201 cgcgccatcg gcgcgaaaca ggatggcgag gtcgagctgg cgcgcgttga gcatcgtcgt
     4261 caggtggccc gacagcgctt cgaccagatg cacgcggacg gccggatagc gctggcccat
     4321 ggcatgcatc agcggcacgc cgagcaccga cgcggtggtc gatgccaggc cgatgctgac
     4381 ccggcccgac aggcgcgccg tctgtgccgc gtgcaccgca tcttcggcat ggcgcaaggt
     4441 gagctgcgcc tgctgcaaaa aggccagtcc ggcgtcggtg ggcagcaccc cggtggcgtg
     4501 gcgttgcagc agccgggtgg ccagctcgcc ctcgagccgg ctgatctgct ggctcagcgc
     4561 cgacgtcgcc atgcccaggt cgagcgccgc cttgcccatg ctgccggcct cgcaaacccg
     4621 gacgaagtaa cgcagctggc gcaattccat ggcggctgta agctatgaat tcaggagctg
     4681 tcggcgcagt ctaaattgaa gcttccgaac ccatggattc ggcagccatc actccatgag
     4741 tggggcacgg cgcgcaaagc gcaccaccgt caccccttcg cgcgcctggc gcaccgaggg
     4801 catgaccagc acgcagtcgt cgtagggggt gaccacgggc tcgccctggc cggggccgtc
     4861 gtggtggccg atcacggtgc cggcgtgggc aaaccgctcc agcccggtga agtcgccggt
     4921 gaaggcaaag cgcgggctgc gcgccaccac cgcaccgtcc accgtcagcg cccactggcg
     4981 cggcgcgtcg ggcagacgcc agccgggcag ggtggcgtcc agcgcggccg gctccagcgt
     5041 gccggcggcc tgcagcaggc gccggcacat gtcctgcgcc acggcgcggc tggccaggtc
     5101 gccatgaaag ccgcattcga tcagcagcga gcgcgcgtcg cccgcctgcg cgtcgggcag
     5161 gccgaagcgc ccgtagtcgc gcaggcgcac gccgtcgctg tggccggcgt cgatgacgat
     5221 gtgctcggg
//
LOCUS       DAOOOG010000170         5217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_19061_length_5217_cov_24.689849, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000170 DAOOOG010000000
VERSION     DAOOOG010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19061_length_5217_cov_24.689849"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..365
                     /locus_tag="PK242_11050"
     CDS             3..365
                     /locus_tag="PK242_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOZ94599.1"
                     /translation="MRRLTQAPNVAIAQLWADLLCEAGYPATVERRFLSSVAGELPPD
                     QCLPEIWLRHDEHLTPARALLAELARVPQRRWRCAACGERVEGGFEQCWACGAWMPDA
                     PDCAPMDSAFGCLEYRSG"
     gene            complement(385..756)
                     /locus_tag="PK242_11055"
     CDS             complement(385..756)
                     /locus_tag="PK242_11055"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HOZ94600.1"
                     /translation="MKAWIIAAACCAAATAWAGEDCAAARTEFETMACLHRVYQTAEQ
                     GLSARYQELVSLLDADGRKRLRVSQLAWLKSRNASCSQRKGTSTLVDPRCMAEVTTAR
                     AQSLQERIGECRAGGCLNERL"
     gene            1023..1826
                     /locus_tag="PK242_11060"
     CDS             1023..1826
                     /locus_tag="PK242_11060"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HOZ94601.1"
                     /translation="MNSGKVRALALRAAFIAPLCLLVGATAQADINVPAGAWASLGGG
                     SMDLGCTDLIVSGNYDLGGGTLTGVRHVTVNSGGSLSFGAGGSVALAGNWANSGTITA
                     TGGTVSIGDDAGCPAPIATSVISGNTTFHNLGITSSAGKLIQFAAGSVQTVEGALTLT
                     GTGVPLRIESTTPGSVSADLRLVPAGTQNLSNLAVRGMSASGQWLAPGQTNQGSGPVS
                     RWFGDPNAAVAPIPTTSQWTLLIMALALGATAVRQRRAKLENSQEGNRS"
     gene            1823..>2064
                     /locus_tag="PK242_11065"
     CDS             1823..>2064
                     /locus_tag="PK242_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94602.1"
                     /translation="MTPRSPLRRLAIAAGVIFAATTAPAFAADVTITPPAGGGVVING
                     PVSMPGVPGSAVQSDKVVCAANGGALGPCPANYGATG"
     assembly_gap    2065..2164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2165..2689
                     /locus_tag="PK242_11070"
     CDS             <2165..2689
                     /locus_tag="PK242_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94603.1"
                     /translation="ATGPAGATGGTGPVGPMGPAGPIGPAGVQGPVGAQGPAGPTGPT
                     GATGAGQAVFFGHSAWDIPTVLTRYIGVGTIDDSEAKVAVPLPIGGTIGGLRVFQSGT
                     AGSGNSWTYTLFVNGSAVGAVSCSISGNAATTCSSAGTVAVSAGDRVALRVAPSSLPS
                     TGRAAGWSFTITSP"
     gene            complement(2761..3852)
                     /locus_tag="PK242_11075"
     CDS             complement(2761..3852)
                     /locus_tag="PK242_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ94604.1"
                     /translation="MARPTICLNMIVKDEAHVIERCLASARPLIDRWCIVDTGSTDDT
                     QQRIQAALAGVPGTLHQRPWKNFGHNRTEALQLALAEGCEHLLFIDADEQFVVPPGFA
                     WPPLTADAHELTCHYAGIQYARRALVATRLPWHWEGVLHEYLDCPQPHRAQALAAPTI
                     LVQHDGARAHDPQTYLKDIAVLQAALAEQPDHPRYQFYLAQSQRDAGQLQAAHASYLR
                     RAALAGWDEERWYALLQAALLAERLQHPPADIAAAYLTAYAARPSRAEPLVQLARWHR
                     QQQQYALACVYAQAACALPLPADRLFVEAAVYQWQALDELAVSAYYVPVPAVQAAGRE
                     AMRKLLRQAQRLPAEVQARLRANARFYGL"
     gene            complement(3916..4476)
                     /locus_tag="PK242_11080"
     CDS             complement(3916..4476)
                     /locus_tag="PK242_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HOZ94605.1"
                     /translation="MALKSTIFKADLQLADIDHGCYTDLQLTLARHPSETDERMMLRL
                     AALALNAHQVHDLCGGDGRIGFGAGLSSPDEPDVQLLDFTGRTRLWVEVGQPDDKPLA
                     KACSKADAVIVYAYGPAAEVWWRAIEGKLTRLDRLQVWRIAAADAQALAALAERSMQL
                     QATVQEGQLTLSAAAGSVTLEPQRWQ"
     gene            complement(4516..4776)
                     /locus_tag="PK242_11085"
     CDS             complement(4516..4776)
                     /locus_tag="PK242_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94606.1"
                     /translation="MIPGLSKTMMLEPPPHRLPGPSYAKPLIDEQGEVRELSAADLKR
                     MHPATAALPASLQTKLRTRGPQKAPTKQRIIIRPSPESASGA"
     gene            complement(4804..>5217)
                     /locus_tag="PK242_11090"
     CDS             complement(4804..>5217)
                     /locus_tag="PK242_11090"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596948.1"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOZ94607.1"
                     /translation="ALLRHLAEKLPISRWQRDLTDSTVLRNMGVALGYAVLAYQSLLT
                     GLNKLELNESQLAADLDHAWEVLAEPIQTVMRRYGVPGAYEKLKEVTRGQQVTREALH
                     GLIRGLDIPAAEKERLLALTPAGYVGKAGELAGRV"
BASE COUNT          721 a         1697 c         1871 g          828 t
ORIGIN      
        1 ccatgcgccg tctgacccag gccccgaacg tggcgatcgc ccagttgtgg gccgatctgc
       61 tgtgcgaggc cggctatccg gccacggtgg agcggcgctt tctgtccagc gtggcgggcg
      121 agctgccgcc cgaccagtgc ctgcccgaaa tctggctgcg ccacgacgag cacctgacgc
      181 ccgcgcgtgc gctgctggcc gagctggcgc gcgtgccgca gcggcgctgg cgctgcgcgg
      241 cctgcggcga gcgggtggaa ggcggcttcg agcagtgctg ggcctgcggg gcctggatgc
      301 ccgacgcgcc cgactgtgcc ccaatggatt cggcctttgg atgcctcgaa tatcgttcgg
      361 gctgagcggc cctgccccgc gcgcctacag gcgttcgttc aggcagccgc cggcgcggca
      421 ttcgccgatg cgctcttgca gtgactgcgc gcgcgccgtc gtcacctcgg ccatgcagcg
      481 cggatcgacc agggtgctgg tgcccttgcg ctgcgagcag ctggcgttgc gcgacttcag
      541 ccacgccagc tggctgacgc gcaggcgctt gcggccgtcg gcgtccagca gcgacaccag
      601 ttcctggtag cgcgcgctca gcccctgctc ggccgtctgg tagacgcggt gcaggcaggc
      661 catggtctcg aattcggtgc gcgcggccgc gcagtcctcg cccgcccagg cggtggcggc
      721 ggcgcagcag gcggcggcga tgatccaggc tttcatggtc ggcgggtgca gcggcggtgg
      781 ccgaggcgtg ttgcccggcg cgggcatctt aatcgggcgg catgtgaccc gaagtgcccg
      841 cgcatgcggc catcgtctcg cgcaattgag gtaaaaagtg tgactttatg ccgccaaatt
      901 gtcgcggatc aacgacaacg acgtgaacac ggtccgcgat cggccggatg gttgtaataa
      961 tgtgaacgag tcgttaccaa aacatgcgct ggctttgtga atgggccagg gaggcatcgt
     1021 caatgaattc aggcaaagtc agggcgctgg ccctcagggc agctttcatc gcaccgctgt
     1081 gcttgctcgt gggcgccacg gcccaggcgg acatcaacgt tcccgccgga gcttgggcca
     1141 gcctgggtgg cggcagcatg gatctgggct gcaccgacct gatcgtcagc ggcaactacg
     1201 atctgggcgg cggcaccctg accggtgtgc gccacgtcac cgtgaacagc ggcggctctc
     1261 tcagcttcgg tgccggcggt agcgttgcgc tggcgggcaa ttgggccaac agcggcacca
     1321 tcaccgccac cggcggcacg gtttccatcg gtgacgatgc cggttgcccg gcgccaatcg
     1381 ccaccagcgt gatcagtggc aacaccacgt tccacaacct cggcatcacc agcagcgccg
     1441 gcaagctgat ccagtttgcc gccggctcgg tgcagacggt ggagggcgcg ctcacgctca
     1501 cgggtacggg tgtgccgctg cgcatcgagt cgaccacgcc gggatcggtc tcggccgacc
     1561 tcaggctggt tcccgctggc acgcagaacc tgagcaacct ggccgtgcgc ggcatgtcgg
     1621 ccagcggcca gtggctggcg cccggccaga ccaaccaggg cagcgggccg gtatcgcgct
     1681 ggttcggcga ccccaatgcc gccgtggcac ccattcccac cacctcgcag tggacgctgc
     1741 tgatcatggc gctggcgctc ggcgcgacgg ccgtgcgtca gcgtcgcgcc aaactcgaaa
     1801 actctcagga aggaaaccgc tcatgacacc ccgctccccc ctgcgtcgcc tggccatcgc
     1861 tgctggcgtc atcttcgcgg ccactacggc cccagccttc gcggccgacg tcaccatcac
     1921 gccaccggct ggcggcggtg tggtgatcaa cggcccggtc agcatgcccg gggtgcccgg
     1981 cagtgccgtt cagtccgaca aggtggtgtg cgccgccaac gggggtgcgc tcggtccctg
     2041 tccagccaac tatggcgcca cgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnngccaca ggccccgccg gtgccacggg tggtacaggt ccggtcggtc ccatgggtcc
     2221 ggcgggtccc atcggtccgg caggcgtgca aggtccggtt ggtgcacagg gaccggctgg
     2281 tcccacgggt ccgacgggtg ctaccggcgc cggccaggcg gttttcttcg ggcactcggc
     2341 atgggacatt cccacggttt tgacgcggta catcggtgtc ggcaccatcg atgacagcga
     2401 ggccaaggtt gcggtgccat tgccgattgg cggcaccatt ggcggcctgc gcgtgtttca
     2461 gagtggcacg gccggcagtg gcaacagctg gacctacacc ctcttcgtca atggctcggc
     2521 ggtgggcgca gtcagttgca gtatcagcgg caacgcggcc acgacctgca gcagcgcggg
     2581 caccgtggcg gtgtcggctg gcgaccgggt ggcgttgcgg gttgcgccca gcagtctgcc
     2641 cagcaccgga cgggcagctg gctggtcatt cacgatcacg tcgccgtaag ccaaagcggg
     2701 aaacccacag caataggggg ccatgcgccc cctttttgcc gtgcatcggg aaaccttgct
     2761 ctacagcccg tagaaacgcg cattggcgcg cagccgtgcc tgtacctcgg ccggcaggcg
     2821 ctgcgcctgc cgcagcagct tgcgcatggc ctcacgtccc gcggcctgca ctgccggcac
     2881 cggcacgtaa taggcgctga cggccagctc gtccagcgcc tgccactggt aaacagcggc
     2941 ttcgacgaac aggcggtcgg ccggcagcgg cagcgcgcag gcggcctggg cgtacacgca
     3001 ggccagggcg tattgctgct gctggcggtg ccagcgcgcc agttgcacca gcggctcggc
     3061 gcggctgggg cgggcggcat aggcggtcag ataggcggcg gcaatgtcgg ccggcgggtg
     3121 ctgcaggcgc tcggccagca gggcggcctg cagcagggcg taccagcgct cctcgtccca
     3181 gccggccagg gcggcgcggc gcaggtagct ggcgtgcgcg gcctgcagct ggccggcgtc
     3241 gcgctggctt tgcgccagat agaactggta gcgcgggtga tcgggctgct cggccagcgc
     3301 ggcctgcagc acggcgatgt ccttcagata ggtctggggg tcgtgcgcgc gcgcgccgtc
     3361 gtgctgcacc aggatggtgg gcgcggccag cgcctgggcg cggtgcggct gggggcagtc
     3421 caggtattcg tgcagcacgc cctcccagtg ccagggcagg cgggtggcga ccagggcgcg
     3481 gcgcgcgtac tggatgccgg cgtagtggca ggtcagctcg tgcgcgtcgg ccgtgagggg
     3541 cggccaggca aagccgggcg gcacgacaaa ctgctcgtcg gcgtcgatga acagcaggtg
     3601 ctcgcagcct tcggccaggg ccagttgcag cgcctcggtg cggttgtggc caaagttctt
     3661 ccaggggcgc tggtgcaggg tgccgggcac gccggccagc gcggcctgga tgcgctgctg
     3721 tgtgtcgtca gtggagccgg tgtcgacgat gcaccagcgg tcgatcagcg gccgggccga
     3781 ggccaggcag cgctcgatca catgcgcttc gtccttgacg atcatgttca ggcagatcgt
     3841 ggggcgggcc atggcgctca ggtgggcaag ggtttgacga tcggggggtg gctgcagggg
     3901 cggatggcgg ggcggctatt gccagcgctg cggctccagc gtcacgctgc cggcggcggc
     3961 agacagggtg agctggccct cctgcaccgt ggcctgcagc tgcatgctgc gctcggccag
     4021 cgccgccagc gcctgcgcgt cggcggcggc gatgcgccag acctggagcc ggtccagccg
     4081 cgtcagcttg ccctcgatgg cgcgccacca cacctcagcc gccgggccgt aggcatagac
     4141 gatcaccgca tcggccttgc tgcaggcctt ggccagcggc ttgtcgtcgg gctggcccac
     4201 ctccacccac aggcgcgtgc ggccggtgaa atcgagcagc tgcacgtccg gctcgtccgg
     4261 gctggacagg ccggcgccaa agccgatgcg accgtcgccg ccgcacaggt cgtgcacctg
     4321 gtgggcgttc agcgccagcg cggccagccg cagcatcatg cgctcgtcgg tctcgctggg
     4381 atggcgcgcc agggtcagct gcaggtcggt gtagcagccg tggtcgatgt cggccagttg
     4441 caggtcggcc ttgaagatgg tggatttgag ggccacggga tgactatgaa ttaggtagct
     4501 gttggcgcat tctggctaag cgccagaggc ggattccggt gacgggcgaa tgatgatgcg
     4561 ctgcttggtc ggggctttct gcgggccgcg ggtgcgcagc ttggtttgca gcgacgcggg
     4621 cagggccgca gtggcggggt gcatgcgctt gaggtcggcg gcgctgagtt cgcggacctc
     4681 gccctgctca tcgatcaggg gtttggcata agaggggcct ggcaggcgat ggggcggcgg
     4741 ttcaagcatc atggtcttcg acaatcccgg aatcactccg gccactcatc ggcccaccgc
     4801 gagtcacacc cgccccgcca attcccccgc cttgcccaca tacccggccg gcgtcagcgc
     4861 cagcagccgc tccttttcgg cggccggaat gtccagcccg cggatcaggc cgtgcagcgc
     4921 ttcgcgcgtg acctgctggc cgcgggtgac ttccttgagc ttttcgtacg cgccgggcac
     4981 gccgtagcgg cgcatcacgg tctggatggg ttcggccagc acttcccagg cgtggtccag
     5041 gtcggcggcg agctgcgact cgttgagttc cagcttgttc aggcccgtca gcaacgactg
     5101 gtaggccagc acggcgtagc ccagggccac gcccatgttg cgcagcacgg tggagtcggt
     5161 caggtcgcgc tgccagcggc tgatgggcag cttttcggcc aggtggcgca gcagggc
//
LOCUS       DAOOOG010000171         5111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_19629_length_5111_cov_26.148339, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000171 DAOOOG010000000
VERSION     DAOOOG010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19629_length_5111_cov_26.148339"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PK242_11095"
     CDS             <1..1307
                     /locus_tag="PK242_11095"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOZ94608.1"
                     /translation="SLQDEIKRDLHACHEHRPELAMVDSAKGITNFHSPNDIIVDASM
                     PAMIRNGGKMWGADGRLKDVKAVMPESTFARIYQEMINFCKWHGAFDPKTMGTVPNVG
                     LMAQQAEEYGSHDKTFEIPEDGVANITDLNTGEVLMSQNVEAGDIWRMCQVKDAPIRD
                     WVKLAVTRARQSGMPVVFWLDPYRPHEAQLITKVKMYLHEHDTSGLQIEIMSQVRAMR
                     YTLERVIRGLDTISATGNILRDYLTDLFPIMELGTSAKMLSIVPLMAGGGMYETGAGG
                     SAPKHVQQLVEENHLRWDSLGEFLALAVSLEDLGIKNNNARAKLLARTLDAATGKLLD
                     NNKGPSPRTGQLDNRGSHFYLAMYWAQALAAQTEDAELAAKFAALAKTLTDNEARIVQ
                     ELTDIQGQAVDIGGYYMPDTAKLAQVMRPSATFNQALAALVG"
     gene            complement(1601..2044)
                     /locus_tag="PK242_11100"
     CDS             complement(1601..2044)
                     /locus_tag="PK242_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOZ94609.1"
                     /translation="MLALSCSAALAQRAPTGQPQLDLPRTTLTAGMHRIEVQLASTPV
                     QRQIGLMHRQSMPAHEGMLFTFDEPGVQCFWMKNTLIPLTAAFVADDGTIVNLADMKP
                     LDESSHCSARPVRYVLEMNQGWFAQRHLKAGHRLGGGPFSKPAAR"
     gene            2554..3807
                     /gene="icd"
                     /locus_tag="PK242_11105"
     CDS             2554..3807
                     /gene="icd"
                     /locus_tag="PK242_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOZ94610.1"
                     /translation="MYQHIQVPAGGQKITVNADGSLNVPDQPIIPFIEGDGTGVDITP
                     VMIKVVDAAVARCYGGRRRIHWMEVYAGEKSTRVYGPDVWLPEETLAALKEYVVSIKG
                     PLTTPVGGGIRSLNVALRQELDLYVCLRPVRYFKGVPSPVKEPEKVDMVIFRENSEDI
                     YAGIEWEARSAKAQQLIKFLVEDMGVAKIRFPATSGIGVKPVSIEGTERLVRKAIQYA
                     IDNDRPSVTLVHKGNIMKHTEGGFRDWGYALAQREFGAELLDGGPWCQFRNPKTGRNI
                     VVKDAIADAFLQQILLRPAEYSVIATLNLNGDYISDALAAQVGGIGIAPGANLSDSVA
                     CFEATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWTEAADLIITSMEAAIQSKRVTY
                     DFARLMEGAVQVSCSGFGQVMIDHM"
     gene            complement(3878..4150)
                     /locus_tag="PK242_11110"
     CDS             complement(3878..4150)
                     /locus_tag="PK242_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOZ94611.1"
                     /translation="MPEGKVKWFNDLKGFGFIEPDGGGPDVFAHFSAIDMDGFKTLKE
                     GARVSYEVNQGPKGLLATRIRTDPMAAVPTPLPNAGALPDQAPLPH"
     gene            4281..4658
                     /gene="clpS"
                     /locus_tag="PK242_11115"
     CDS             4281..4658
                     /gene="clpS"
                     /locus_tag="PK242_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOZ94612.1"
                     /translation="MIFMASTPPTRSPALPGPVTPVRQPEGGGEVVLERVPQKVKPPQ
                     MYQVVMLNDDYTPMEFVVVVIQEFFGKDRESATQIMLKIHLDGRGVCGVYSRDVAATK
                     VEQVLDAARQAGHPLQCVSEPVE"
     gene            4799..>5111
                     /locus_tag="PK242_11120"
     CDS             4799..>5111
                     /locus_tag="PK242_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOZ94613.1"
                     /translation="MIAQELEVSLHMAFVEARQQRHEFITVEHLLLALLDNPSAAEVL
                     RACAANIDDLRKSLTNFIKDNTPQVAGSDEVDTQPTLGFQRVIQRAIMHVQSTGNGKK
                     EV"
BASE COUNT          931 a         1683 c         1719 g          778 t
ORIGIN      
        1 agagcctgca ggacgagatc aagcgcgacc tgcacgcctg ccacgagcac cgccctgagc
       61 tcgccatggt cgattcggcc aagggcatca ccaacttcca ctctcccaac gacatcatcg
      121 tcgacgcctc catgcccgcc atgatccgca acggcggcaa gatgtggggc gccgacgggc
      181 gcctgaagga cgtcaaggcc gtgatgcccg aatcgacctt tgcccgcatc taccaggaga
      241 tgatcaactt ctgcaagtgg catggcgcct tcgatcccaa gaccatgggc acggtgccca
      301 acgtcggcct gatggcgcag caggccgagg aatacggcag ccacgacaag accttcgaga
      361 tccccgagga cggcgtggcc aacatcaccg acctgaacac cggcgaggtg ctgatgagcc
      421 agaacgtcga ggccggtgac atctggcgca tgtgccaggt caaggacgcg ccgatccgcg
      481 actgggtcaa gctcgccgtc acgcgcgcgc gccagtcggg catgccggtg gtgttctggc
      541 ttgacccgta ccgtccgcac gaggcgcagc tcatcaccaa ggtcaagatg tacctgcacg
      601 agcacgacac cagcggcctg cagatcgaaa tcatgagcca ggtgcgtgcc atgcgctaca
      661 cgctggagcg ggtgatccgc ggcctggaca ccatcagcgc caccggcaac atcctgcgcg
      721 actacctgac cgacctgttc cccatcatgg agctgggcac cagcgccaag atgctgtcca
      781 tcgtgccgct gatggccggc ggcggcatgt acgaaaccgg cgccggcggc tcggcgccca
      841 agcacgtgca gcagctggtg gaggaaaacc acctgcgctg ggacagcctg ggcgagttcc
      901 tggccctggc cgtgagcctg gaagatctgg gcatcaaaaa caacaacgcc cgcgccaagc
      961 tgctggccag gacactggac gccgccaccg gcaagctgct ggacaacaac aagggcccca
     1021 gcccgcgcac cggtcagctg gacaaccgcg gcagccactt ctacctggcc atgtactggg
     1081 cgcaggcgct ggcggcgcag accgaggacg ccgagctggc ggccaagttc gcggcgctgg
     1141 ccaagacgct gaccgacaac gaggccagga tcgtgcagga actcaccgac atccagggcc
     1201 aggcggtcga catcggcggc tactacatgc ccgacacggc caagctggcg caggtgatgc
     1261 gccccagcgc caccttcaac caggcgctgg cggcgctggt cggctgagac caccaggtgc
     1321 gctgcgccgc cgtggcggcg gcgcagcgga cccgggcgcg gtggactcac cgcgccccaa
     1381 ttttttgcgc ggcagcggcc gcaggcggcg cccagggtgt tcgttggggt attggccttt
     1441 tctggcctcg ggtcaggcgc agcatgcgcc gcaagctatc gaaatgatag ctgtggcaac
     1501 gctgaaccag cttttcgggc gcgtcgcctt cctgccttgg gcggcttgcg ccttgctcat
     1561 gcagggcgcg ccgcggtgcc ggaaccgatg cggcgtggcc tcagcgcgcc gccggtttgc
     1621 tgaacggccc gccgcccagg cggtgtccgg ccttcaggtg gcgttgggcg aaccagcctt
     1681 ggttcatctc cagcacgtaa cgcaccggcc tggccgagca atggctgctt tcatccagcg
     1741 gcttcatgtc ggccaggttg acgatggtgc cgtcgtcggc cacgaaggcg gcggtgagag
     1801 ggatcagcgt gttcttcatc cagaagcact gcacgcccgg ctcgtcgaag gtgaacagca
     1861 tgccctcgtg cgccggcatg ctctggcggt gcatcaggcc gatctggcgc tgcaccggcg
     1921 tgctggccag ctgcacctcg atgcggtgca tgccggccgt gagcgtggtg cgcggcaggt
     1981 ccagttgcgg ttgaccggtc ggcgcgcgct gggcgagcgc ggccgagcag gacagggcca
     2041 gcatgagcag gtgcaggggg cggcaggcga gaacgggcat ggcgacagcg gggatgggaa
     2101 caagggatcg ggcgtcggcg gcgacccgaa ggtggggccc gcgccggcgt ccagcgcgca
     2161 ttgtcgccgc cacgcgcttt gccctggcgt gcgccgcaag gcggtggttg gtgtggtgcg
     2221 ccgctatggc ttgcactttc agcgcgtcgc tgagcggctc gatgcaagtc gcgcacgcgc
     2281 agcaatgcgt gcgcctttca agcgggcgca acgccgcagg cagtcgctca gcggctcgcc
     2341 caaagggagc ccacggaagg cgcccatgcc gcgttgcagc ccttgccaag ggatctgcca
     2401 tgggctgcgg gctgcgcctt gcctggacgc cttctggggt ctctgaaagc acaagccata
     2461 gcggcgcacc acactagaat cgattccgcc ccgagcgcca tgccagccgg cgtgcgcgcc
     2521 caggctggcc catcaacctt ggagaaccca tccatgtacc agcacatcca ggtgcctgcc
     2581 ggcggacaga agatcaccgt caacgccgat ggctcgctca acgttccgga ccagcccatc
     2641 atccccttca tcgaaggtga cggcacgggc gtggacatca cgccggtgat gatcaaggtg
     2701 gtggatgccg cggtggccag gtgctatggc gggcggcgca ggatccactg gatggaggtg
     2761 tatgcgggcg agaaatccac ccgtgtctac ggccccgacg tctggctgcc cgaagagacg
     2821 ctggcggcgc tcaaggagta cgtggtctcc atcaaggggc cgctgaccac cccggtcggc
     2881 ggcggcatcc gctcgctcaa cgtggcgctg cgccaggagc tcgatctgta cgtctgcctg
     2941 cgcccggtgc gctacttcaa gggcgtgccc tcgccggtga aggagccgga gaaggtcgac
     3001 atggtgatct ttcgcgagaa cagcgaggac atctacgccg gcatcgaatg ggaggccagg
     3061 agcgccaagg cgcagcagct catcaagttc ctggtcgagg acatgggtgt ggccaagatc
     3121 cgctttccgg ccacctccgg catcggcgtc aagccggtgt cgatcgaagg caccgaacgc
     3181 ctggtgcgca aggccatcca gtacgccatc gacaacgaca ggcccagcgt caccctggtg
     3241 cacaagggca acatcatgaa gcacaccgaa gggggcttcc gtgactgggg ttacgcgctg
     3301 gcgcagcgcg aattcggggc cgaactgctg gacggcgggc cgtggtgcca gttcaggaac
     3361 ccgaagacgg gccgcaacat cgtcgtcaag gacgccatcg ccgatgcctt cctgcagcag
     3421 atcctgctgc gtccggccga gtattcggtg atcgccacgc tgaacctgaa cggcgactac
     3481 atcagcgacg cgctggcggc gcaggtgggc ggcatcggca tcgcgccggg cgccaacctg
     3541 agcgacagcg tggcctgctt cgaagccacc catggcacgg cgcccaagta cgccggcaag
     3601 gactacgtca accccggctc cgaaatcctc agcgccgaaa tgatgctgcg ccacatgggc
     3661 tggaccgagg cggccgacct gatcatcacc agcatggaag cggccatcca gagcaagcgc
     3721 gtcacctacg acttcgcccg cctgatggaa ggcgcggtgc aggtcagctg ctcaggcttc
     3781 ggtcaggtga tgatcgatca catgtgagcg ccgccggccc aaaaaaagat cccgagccgg
     3841 gctcgggatc ggggtgagga ggtgaccggc tggccggtca atgcggcagg ggcgcctggt
     3901 cgggcagcgc gccggcgttc ggcaggggcg tgggcaccgc cgccatcgga tcggtgcgaa
     3961 tccgcgtcgc cagcaggccc ttggggccct ggttgacttc atagctgacg cgcgcgcctt
     4021 ccttcagggt cttgaacccg tccatgtcga tggccgagaa atgtgcgaac acgtcgggcc
     4081 cgccgccgtc cggctcgatg aagccgaaac ccttcaggtc attgaaccac ttgacttttc
     4141 cttcgggcat gtcttgtctc ctgatgtctt ccgtccgccg gcaagtgtgc gggaagggtt
     4201 ttgcccttgt caacccgagg cgccgtccgg gcgggcgcgg aaagccctgc gcccgtcggc
     4261 gggtaggcgc gctcattaga atcattttca tggcgagcac tcctccgaca cgttccccgg
     4321 ccctgccggg gcccgtcacc cccgttcggc aacccgaagg tggcggcgag gtggtgctcg
     4381 agcgggtgcc gcagaaggtc aagccgccgc agatgtacca ggtggtgatg ctcaatgacg
     4441 actacacgcc gatggaattc gtggtcgtgg tgatccagga attcttcggc aaggaccgcg
     4501 agagcgccac ccagatcatg ctcaagatcc acctcgacgg ccgcggcgtg tgcggcgtct
     4561 attcgcggga tgtggcggcg accaaggtcg agcaggtgct ggacgccgcg cgccaggcgg
     4621 ggcacccgct gcagtgcgtc agcgagccgg tggaataggc acctggggcc ggcgccgcct
     4681 ggaagacgaa ccgaagcagg agccggacca tgccgttgaa tatcaccgaa cttcccccaa
     4741 ctgtcgctgt caaaacctcg ggaaagtcgt aagcttgagg tgctagagga accacaccat
     4801 gatcgcccag gaactggaag tcagtctgca catggccttt gtcgaggccc ggcagcagcg
     4861 ccatgagttc atcaccgtcg agcacctgct gctggccctg ctggacaacc cgagcgccgc
     4921 cgaggtgctg cgcgcctgcg cggccaacat cgatgacctg cgcaagtcgc tgaccaactt
     4981 catcaaggac aacacgcccc aggtggcggg cagcgacgag gtggacaccc agccgacgct
     5041 gggcttccag cgcgtgatcc agcgcgccat catgcacgtg cagtccaccg gcaacggcaa
     5101 gaaggaagtc a
//
LOCUS       DAOOOG010000172         5074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_19822_length_5074_cov_24.242080, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000172 DAOOOG010000000
VERSION     DAOOOG010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19822_length_5074_cov_24.242080"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="PK242_11125"
     CDS             <1..152
                     /locus_tag="PK242_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOZ94614.1"
                     /translation="GRLNKQIADDLGISIKTVEAHRANIMEKLGANTVADLLKIALGQ
                     QTAKV"
     gene            235..1089
                     /gene="folD"
                     /locus_tag="PK242_11130"
     CDS             235..1089
                     /gene="folD"
                     /locus_tag="PK242_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOZ94615.1"
                     /translation="MTAQLIDGNALAATVRAEVAHRVQALQARGVQPHLSIILVGDNP
                     ASQVYVRSKVADSQQTGLSATLERYPAELPQAELLARIAALNADPAVHGILVQLPLPP
                     HMDAHAVIEAIAPAKDVDGFHVASAGALMVGQPGFRPCTPYGCMRMLQSIGLHDLRGR
                     HAVVIGRSNIVGKPMAMMLLAQNATVTVCHSGTSDLAALTRQADIVVAAVGKRNVLTA
                     DMVKPGAVVIDVGMNRDDAGKLCGDVDFTGVRQVASWITPVPGGVGPMTRAMLLVNTI
                     EAAERAAA"
     gene            1234..3264
                     /locus_tag="PK242_11135"
     CDS             1234..3264
                     /locus_tag="PK242_11135"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOZ94616.1"
                     /translation="MTNPLLDFSDLPRFDQIRPEHVAPAIDQLLAEAEAALEAAVSPD
                     SPADWTWLSRTLDVPGERLGRAWSVVSHLNAVMDTPELRAAYNAALPRVTEFWTRLGS
                     DERLYARYKAIDPAPLDAAQRRARDNALRDFVLGGAELQGAAKQRYAAIQERLAELSQ
                     KFSENALDATDAYACYASEAEVEGLPPDVLATARAAAQADGQSGYKLTLKLPSYLPAM
                     QFAHDRALRQRLYRAYVTRASDQAEGDATRFDNAPLIREILALRHEEAQLLGYASYGD
                     VSLVPKMAESPRQVIDFLRQLATKARPYAERDVADLRAFARERLDLADPQSWDWPYLG
                     ERLKEARYAFSEQEVKQYFQAPKVLAGLFKIVETLFEVAIRRDAAPVWHPSVEFYRIE
                     RDGQLLGQFYLDPGARTGKRGGAWMDDVRARWLRPDNGRLQTPVAQLVCNFAEGVHGA
                     PPLLTHDDVITLFHEFGHGLHHLLTQVNERDVSGISGVEWDAVELPSQFMENFCWEWE
                     VLRHMTAHVDSGEPLPRELFDRMIAARNYQAGLQTVRQIEFALFDMLLHTEHASGQDF
                     QPLLDAVRREVAVLQPPPFARTAQTFSHIFAGGYAAGYYSYKWAEVLSADAYAAFEES
                     VTPEGLPSVETGRKYRREILEAGGSRPAMESFKAFRGREPSLDALLRHQGMA"
     gene            3324..3971
                     /locus_tag="PK242_11140"
     CDS             3324..3971
                     /locus_tag="PK242_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOZ94617.1"
                     /translation="MHRRHPLLLVTALSALLLAGGVQAQVYRSSGPGGVSYSDQPPPA
                     QAGRQVAPLGGDTRRSGSATLPYTLSQTVQRFPVTLYTSADCPPCVSGRNLLIQRGVP
                     FSEKTVQSNEDIAALQRLAGASQVPVLTIGGQRLSGYSDQEWSQYLDAAGYPRTSQLP
                     AAYQRPAATPLVAQSPVPAAATGTAREATAPAAATPAEPSIAPPRNATNPAGIRF"
     gene            complement(4085..4315)
                     /locus_tag="PK242_11145"
     CDS             complement(4085..4315)
                     /locus_tag="PK242_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010351400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HOZ94618.1"
                     /translation="MFPEYRDLITRLKGEGSNTRFLNLFERHNELDHQIKAMEGHDAG
                     AIHSEIETLKKEKLRVKDELYTILRQEATVGK"
     gene            complement(4344..>5074)
                     /locus_tag="PK242_11150"
     CDS             complement(4344..>5074)
                     /locus_tag="PK242_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HOZ94619.1"
                     /translation="LAPGFNMLDPIKATIVTPGLNLDGKFDKSGIPASIVTKFLAEHG
                     VVVEKTGLYSFFIMFTIGITKGRWNTLLTALQQFKDDYDRNQPMWRIMPEFCAQHRRY
                     EKMGLRDLCQHVHQLYAKYDIARLTTDMYLSELTPAMNPADAYACIARRQVERVEIDQ
                     LEGRITTALVTPYPPGIPLLIPGEVFNRKIVDYLKFARDFALECPGFETDIHGLVEVE
                     DDHGHVRYFADCVAADARGVRGGL"
BASE COUNT          832 a         1831 c         1638 g          773 t
ORIGIN      
        1 ccggccgcct gaacaagcag atcgccgacg acctgggcat cagcatcaag accgtcgagg
       61 cgcaccgcgc caacatcatg gaaaagctgg gcgccaacac cgtggccgac ctgctcaaga
      121 tcgccctggg gcagcaaacc gccaaggtct gagctactat cgttttcata gctgccggcg
      181 catatccatc aagcgccagg cagctttttc attggtatcc gacgggttct tcacatgacc
      241 gcccaactga tcgacggcaa cgccctggcg gccaccgtcc gcgccgaggt ggcgcaccgc
      301 gtgcaggcgc tgcaggcacg cggcgtgcaa ccgcacctga gcatcattct ggtcggtgac
      361 aacccggcca gccaggtcta cgtgcgcagc aaggtggccg acagccagca gaccggcctg
      421 tcggcgacgc tggaacgcta cccggccgag ctgccgcagg ccgagttgct ggcgcgcatc
      481 gccgccctca acgccgaccc ggccgtgcac ggcatcctgg tgcagctgcc gctgccgcca
      541 cacatggacg cgcatgcggt gatcgaggcc attgccccag ccaaggacgt ggacggcttt
      601 cacgtcgcca gcgccggcgc gctgatggtg ggccagcccg gctttcgccc ctgcaccccc
      661 tacggctgca tgcgcatgct gcagtcgatc ggcctgcacg atctgcgggg ccggcacgcg
      721 gtggtgatcg ggcgcagcaa catcgtcggc aagccgatgg ccatgatgct gctggcgcag
      781 aacgccaccg tcaccgtctg ccacagcggc acctccgacc tggcggcgct gacgcgccag
      841 gccgacatcg tggtggccgc cgtcggcaag cgcaacgtcc tgacggcgga catggtcaag
      901 cccggcgcgg tggtcatcga cgtcggcatg aaccgcgacg acgccggcaa gctgtgcggc
      961 gacgtggact tcaccggggt gcgccaggtg gccagctgga tcaccccggt gcccggcggc
     1021 gtgggaccca tgacgcgcgc catgctgctg gtcaacacca tcgaggccgc cgagcgcgcc
     1081 gccgcctgac ccggggccgc gcggcgccgg ccgcccgcag accctcgatc tttaagcaaa
     1141 atggcctgcg gcgcttgtcc acaaagcgcc agaagctatc attttgatac tttgcggctt
     1201 gacaaacacc gccttgtccc cagctgaagc gccatgacca atcccctgct cgacttctcc
     1261 gacctgccgc gcttcgacca gatccgcccg gaacacgtgg ctcccgccat cgaccagctg
     1321 ctggccgaag ccgaggcggc gctggaggcc gccgtcagcc ccgactcccc ggccgactgg
     1381 acgtggctgt cgcgcacgct cgacgtgccc ggcgagcgcc tgggccgcgc ctggagcgtg
     1441 gtcagccacc tcaacgccgt catggacacc cccgagctgc gcgccgccta caacgccgcc
     1501 ctgccccggg tcaccgagtt ctggacccgg ctgggcagcg acgagcggct gtacgcccgc
     1561 tacaaggcca tcgacccggc gccgctggac gccgcgcagc ggcgcgcgcg cgacaacgcc
     1621 ctgcgcgact tcgtgctggg cggcgccgag ctgcagggtg cagccaagca gcgctacgcc
     1681 gccatccagg agcgtctggc cgagctgagc cagaagttca gcgagaacgc gctcgacgcc
     1741 accgacgcct acgcctgtta tgccagcgaa gccgaggtcg aaggcctgcc gcccgatgtc
     1801 ctggccacgg cccgcgccgc cgcccaggcc gacggccagt ccggctacaa gctcaccctg
     1861 aagctgccca gctacctgcc ggcgatgcag ttcgcgcatg accgcgcgct gcgccagcgc
     1921 ctgtaccgcg cctacgtcac gcgcgcctcc gaccaggccg agggcgatgc cacgcgcttt
     1981 gacaacgcac cgctgatccg cgaaatcctg gcgctgcgcc acgaagaagc gcagctgctg
     2041 ggctacgcca gctacggcga cgtgtccctg gtgcccaaga tggccgaatc gccgcgccag
     2101 gtgatcgact ttctgcgcca gctggcgacc aaggcccggc cctacgccga gcgcgacgtg
     2161 gccgacctgc gcgccttcgc gcgcgaacgg ctggacctgg ccgatccgca gagctgggac
     2221 tggccctatc tgggcgaacg gctcaaggaa gcgcgctacg ccttcagcga gcaggaggtc
     2281 aagcagtact ttcaggcgcc caaggtgctg gccgggttgt tcaagatcgt cgagaccctg
     2341 ttcgaagtgg ccatccgccg cgacgcggcg ccggtctggc acccctcggt cgagttctac
     2401 cgcatcgagc gcgacgggca actgctgggc cagttctatc tcgaccctgg cgcgcgcacc
     2461 ggcaagcgcg gcggcgcctg gatggacgac gtgcgcgccc gctggctgcg cccggacaac
     2521 ggccgcctgc agacccccgt ggcccagctg gtgtgcaact tcgccgaggg cgttcatggc
     2581 gcgccgccgc tgctcacgca cgacgacgtc atcaccctgt tccacgaatt cggccacggc
     2641 ctgcaccatc tgctgacgca ggtgaacgag cgcgacgtct cgggcatcag cggcgtcgag
     2701 tgggatgcgg tcgagctgcc cagccagttc atggagaact tctgctggga atgggaagtg
     2761 ctgcgccaca tgacggccca tgtcgacagc ggcgagccgc tgccgcgcga gctgttcgac
     2821 aggatgatcg ccgcccgcaa ctaccaggcc ggcctgcaga ccgtgcgaca gatcgagttc
     2881 gccctgttcg acatgctgct gcacaccgag cacgccagcg gccaggactt ccagccgctg
     2941 ctggacgcgg tgcgacgcga agtggcggtg ctgcagccgc caccgttcgc gcgcacggca
     3001 cagaccttca gccacatctt cgctggcggt tacgccgcgg gctactacag ctacaagtgg
     3061 gccgaagtgc tgtcggccga cgcctacgcc gcgttcgagg aaagcgtgac gcccgagggc
     3121 ttgcccagcg tcgaaaccgg acgaaaatac cgacgcgaga ttctggaagc cggcggcagc
     3181 cgcccggcca tggaatcgtt caaggccttc cgtggccgcg agcccagcct ggacgcgctg
     3241 ctgcgccacc agggcatggc ttgaggcgca cggcggccgg cccgcagccc ttgtcgtcca
     3301 ccccgcccgc aggagaatcg cccgtgcatc gtcgtcaccc tctgcttctc gtcacggcct
     3361 tgagcgccct gctgctggcg ggcggcgtcc aggcgcaggt gtaccgttcg agcgggcccg
     3421 gcggcgtcag ctacagcgac cagccgcctc ccgcacaggc gggtcgccag gtggccccgc
     3481 tcggcggcga tacccggcgc agcggcagcg ccacgctgcc ctacacgctc agccagaccg
     3541 tgcagcgctt tcccgtcacg ctctacacct ccgccgattg cccgccctgc gtcagcggcc
     3601 gcaacctgct gatccagcgc ggcgtgccgt tcagtgaaaa aaccgtgcag tccaacgagg
     3661 acatcgcagc cctgcagcgg ctggccggtg ccagccaggt gccggtgctg accatcggcg
     3721 gccagcgcct gagcggctat tcggaccagg agtggagcca gtacctcgac gccgccggct
     3781 atcccagaac ctcgcagttg ccggcggcct atcagcgccc ggccgccaca ccgctggtgg
     3841 cccaaagccc cgtgcccgcc gcggccaccg gtacggcccg cgaagccact gcccccgccg
     3901 cggcaacgcc ggccgagccc agcatcgccc ccccgcgcaa cgccaccaac ccggccggga
     3961 ttcgcttctg aaccgctacc aattcgatag cttctggcgc ttgccagata tgcgccagag
     4021 cacataaaag cccacaaaaa acccgcctgg gcgaggcggg tttgtgcggc gcgcgatggg
     4081 cggctcactt gccgaccgtg gcctcctgcc gcaggatcgt gtacagctcg tccttgacgc
     4141 gcagtttttc cttcttcagc gtctcgatct cgctgtggat ggcaccggca tcgtggcctt
     4201 ccatggcctt gatctggtga tccagttcgt tgtgccgctc gaacaggttc aggaaacggg
     4261 tgttcgagcc ctcgcccttg agacgggtga tcagatcgcg gtattcagga aacatcggtt
     4321 ctcctcagtg gtcacacgct tcatcataaa ccgccgcgca ccccacgggc atcggcggcc
     4381 acgcaatcgg caaagtagcg cacatggccg tggtcgtctt ccacctccac caagccgtgg
     4441 atgtcggtct cgaagccggg gcactcgagc gcgaagtcgc gcgcgaactt caggtagtcg
     4501 acgatcttgc ggttgaacac ctcgcccgga atcagcagcg gaatgccggg cggatagggc
     4561 gtcaccagcg ccgtggtgat gcggccttcg agctggtcga tctccacccg ctccacctgg
     4621 cggcgggcga tgcaggcgta ggcgtcggcc gggttcatgg ccggcgtcag ctcgctcagg
     4681 tacatgtcgg tggtcaggcg cgcgatgtcg tacttggcgt agagctgatg cacgtgctgg
     4741 cacaggtcgc gcaggcccat cttctcgtag cgccggtgct gggcgcagaa ctccggcatg
     4801 atgcgccaca tcggctggtt gcggtcgtag tcgtccttga actgctgcag cgccgtcagc
     4861 agcgtgttcc agcggccctt ggtgatgccg atggtgaaca tgatgaagaa gctgtagagc
     4921 ccggtctttt ccaccaccac gccgtgctcg gccaggaatt tggtgacgat cgacgccgga
     4981 atgccgctct tgtcgaactt gccgtccagg ttcaggccgg gcgtgacgat ggtggccttg
     5041 atcgggtcca gcatgttgaa gcccggcgcc agct
//
LOCUS       DAOOOG010000173         5063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_19876_length_5063_cov_25.654952, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000173 DAOOOG010000000
VERSION     DAOOOG010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19876_length_5063_cov_25.654952"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..818)
                     /locus_tag="PK242_11155"
     CDS             complement(138..818)
                     /locus_tag="PK242_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ94620.1"
                     /translation="MNRPTVPTMALLARTPLFSSLSPPALERLAAATLTLRPRSGEVL
                     FDQGEPARGIYLVVYGDIKLISTTPSRGRRLTGVAHAGESFGEPVLFLERPTLVRAQA
                     ACDALVLMVPGEAVFCELDCNPKFARQMIAGLSRRVQSLVSELDQQAIGSGSARFIAY
                     LTREAHGAQSLSWTLPATKSQVASQLNVTPEHFSRILRDLATAGLLQVQGRRIIVPDM
                     ERLQQSVW"
     gene            complement(815..1492)
                     /locus_tag="PK242_11160"
     CDS             complement(815..1492)
                     /locus_tag="PK242_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94621.1"
                     /translation="MDTEPRAAIVNPTFSWYRATAMRTPAFYDEVEPIVLVDPLAALL
                     GAVDGGLIEYRYLDAVKLAGHSCPTVACAWLMTGTALARLYPDGLPERGNVHVELRQA
                     ADLGVTGVIASIASLVTGAATESGFKGLAGRHGRRNLLRFGVPMQGEIRYTRLDNGQT
                     VELIHQPQAVPRPPGLTEQMRMALAPGADAAVRAAFAQSWQEWVRTMLIDHAHDPALI
                     TVLHSGR"
     gene            complement(1482..1964)
                     /locus_tag="PK242_11165"
     CDS             complement(1482..1964)
                     /locus_tag="PK242_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94622.1"
                     /translation="MIPALAVAALTAGLGATAMAQTKGHDHGHDSAAPHVLSLNNGSK
                     WRTDAPLRAGMKKIRNLVAPELNAAHAGKLDAARYQQLATRIETEVAGIVANCKLEPE
                     ADAMLHLVIADITQGTDAMAGKDHQSRPVLGLTKVARAVNQYGSHFDDPGFKPLADGH
                     "
     gene            complement(2214..2960)
                     /locus_tag="PK242_11170"
     CDS             complement(2214..2960)
                     /locus_tag="PK242_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ94623.1"
                     /translation="MNAAPRTPPSTTDPVTPKHCSPQLRHRILSQARYFSGLSAEDLE
                     QVNRHFRELHYPAESIICHEGGNAERLFFVARGKVKLLRHSSGGDDVLLDLLAQGALF
                     GGLTPLGTRHYPETAMAQTDTCVLAISDGDFQRLLEQHPQVTLAVLASVARSLDDARE
                     VIRQISTSDVRTRVATALLKLAERLGDADEQGILIRAPLSRQDLAAMVGATPETVSRV
                     MADLKREGLIDTGRQWVRVLDSGALRAIDA"
     gene            complement(2992..4962)
                     /locus_tag="PK242_11175"
     CDS             complement(2992..4962)
                     /locus_tag="PK242_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOZ94624.1"
                     /translation="MSISHSVSRLQTLRASPAQRRGLLTLVSGALGLGGVLSAFLDGP
                     AALTATLWIVAALIAGSDIAVRAWHSLRARSASIELLVTIAAVGAIAIGEYWEAAAVT
                     FLFLLGGYLEARTIAKTRSALKDLIDLAPTEVTVRRDGTELVLAPHQVLRGDTVIVRP
                     GGRIGVDGTVLSGRAAVNQAAITGESMPEDKSEGSSVFAGTVSHDGYLEIRAEGVGAD
                     TTLAHIIQRVEQAQEAKAPIQRFIERFAQWYTPAIIVLTILTYALTGDIELALTLLVV
                     ACPGALVISTPISVIAGIGQAARQGVLIKGGEHLETAARIRAVAFDKTGTLTVGRPAV
                     TSIVPLAALQPVPGIGQDDRKQQLLRWAAVAEGGSEHPLARAILREVKGDEPIAAADN
                     FETFAGGGIGAGWQGRRIEIGSPDWLRQRVADWPTDAQAALEEICQRGETLAAISVDG
                     KVFGLLGFADQVRPEAAAALQQLRDGGVRRLVMLTGDHLASAQRVARTLGITEVQAGL
                     LPEHKLKAIERLRTEAGVTAMVGDGINDAPALAAADVGVAMGAAGTDVAIDTADIALM
                     TDDLERVARAITLARATVRNIRQNTGIALLTVAGLLAGVFAGEVHMAGGMFIHQASVL
                     LVILNGMRLMRPRGGNKAPRATAGADMAVSRA"
BASE COUNT          802 a         1661 c         1757 g          843 t
ORIGIN      
        1 tgtgttcatc gtgctgccgg tgccaagccc gggatgcagg ctcgaccggc ttggagcgcg
       61 cggccccaga tcgcgcaggg cacgttcgcg agcctggacc ttgctgttca ttccgtggcc
      121 tgcaggtacc ctcgaactca ccagacgctt tgttgcagcc gctccatatc aggcacgatg
      181 atgcggcgac cctggacttg cagcaggccg gcggtggcca ggtcgcgcag aatgcgcgag
      241 aagtgttcgg gcgtcacgtt gagttgcgac gcgacctggc ttttcgtggc cggcagtgtc
      301 caggagaggc tttgcgcgcc atgtgcctcg cgagtcaggt aggcgatgaa acgcgcgctg
      361 ccactgccga tggcctgctg gtcgagctca ctcacgagag actgcacgcg ccgactcagg
      421 ccggcgatca tctgccgcgc gaacttcgga ttgcaatcca gttcacagaa cacggcctcg
      481 cctggcacca tcagcacaag ggcatcgcat gctgcctgtg cgcgtaccag cgtcggccgc
      541 tcgagaaaca gcactggttc accaaagctt tctccggcgt gcgccacccc ggtcaggcga
      601 cggcctcgtg acggggtggt cgagatcagc ttgatgtcgc catagaccac cagatagatg
      661 ccgcgcgccg gctcaccctg gtcgaacagt acctcgcctg acctgggccg cagggtgagg
      721 gttgcggcag ccaggcgctc gagagcaggg ggcgacaaac tactgaacag cggcgtccgt
      781 gccagcaacg ccatggtagg gacggtgggg cgattcatcg gccggaatga agcaccgtga
      841 tcaatgcagg gtcatgggca tggtcgatca gcatcgtgcg tacccattcc tgccacgact
      901 gggcgaaggc cgcgcgaacc gctgcatctg caccaggtgc gagtgccatg cgcatctgct
      961 cggtcagccc cggcggtcga ggcacggcct gtggctggtg gataagttcc accgtctgtc
     1021 cgttgtccag tcgcgtgtag cgaatttcgc cttgcatcgg taccccgaaa cgcagcagat
     1081 tccggcgccc gtggcggccc gccaagccct tgaagccgct ttcggttgcg gcgcctgtga
     1141 ccaggctggc gatgctggcg atgacccccg tcacgcctag gtcggcggcc tgccgcagtt
     1201 cgacgtgcac gttgccgcgt tcgggcaggc catccgggta caggcgtgcc agtgccgtgc
     1261 cggtcatgag ccaggcgcag gccacggtgg ggcaggagtg ccccgccagc ttgacagcat
     1321 ccaggtagcg atattcgatc aggccaccat caaccgcgcc aagcagggca gccagtggat
     1381 cgaccagcac gatcggttcc acctcatcgt agaaagcggg ggtgcgcatc gccgtggccc
     1441 ggtaccaaga aaaggtgggg ttcacaatgg ccgcccgtgg ctcagtgtcc atcggccagg
     1501 ggtttgaagc cggggtcatc gaaatggctg ccgtactggt tgacggcgcg tgccaccttc
     1561 gtcaggccga gtaccggacg cgactggtgg tccttgccag ccatggcgtc ggtgccctgg
     1621 gtgatgtcgg cgatcaccaa atgcagcatg gcgtcagcct cgggttcaag cttgcagttg
     1681 gcgacgatgc cagccacctc ggtctcgatt cgggttgcga gctgctggta gcgcgccgcg
     1741 tccagcttgc cagcgtgggc cgcgttcagc tcgggcgcca ccaggttgcg gatttttttc
     1801 atgccggcgc gcagcggtgc gtcggtccgc catttgctgc cgttgttgag gctgagcacg
     1861 tgcggtgcgg cactgtcgtg gccgtggtcg tgccccttcg tctgtgccat ggcggttgcg
     1921 ccaagccctg ccgtgagtgc ggcgacagcc agcgctggga tcaagatgtt gcgaatcgat
     1981 gtcttcatgg gggtctctct ccagtgaaac gacgggtacc gatcgcccgc ctgagcttat
     2041 tgacgctgtg cccggggcgg ggttcgatca agttccggcg gtcttcggaa atatcaagaa
     2101 aatgacggtt tgcgtgcgct ggcagcttgc tggcgcgtca ggtcgcgacc ggctgccagt
     2161 gatgcggcgg tggcctgaca tcagcggtca cggctcggcg cgacggccgg acctcaggca
     2221 tcgatggcgc gcagagcccc cgaatccagg acgcggaccc actggcgtcc agtgtcgatc
     2281 aggccctcgc gcttcagatc tgccatgacg cggctcacgg tttcaggcgt ggcaccgacc
     2341 atggccgcga ggtcctggcg tgacagcggc gcacggatca ggatgccctg ttcatcggcg
     2401 tcgcccaagc gctcggccag cttgagcagg gccgtggcga cgcgggtgcg tacgtcggag
     2461 gtactgatct ggcggatcac ttcgcgcgca tcatccagac tgcgtgcaac gctggcaagc
     2521 acggccaggg tcacctgcgg gtgctgctcc agcaaccgct gaaagtcgcc gtcggagatg
     2581 gccagtacgc aggtgtcggt ttgtgccatc gcggtttccg ggtaatgccg ggtgcccagc
     2641 ggcgtcaggc cgccaaacag cgcgccctgc gccagcaggt ccaacagcac gtcgtcgcca
     2701 cccgagctat ggcgcaatag tttgaccttg ccacgggcga cgaagaacag gcgctcggcg
     2761 ttgccgccct cgtggcagat gatcgattcg gccgggtagt gcagctcgcg aaagtgccgg
     2821 ttcacctgct ccaggtcttc ggccgaaagc ccgctgaaat agcgggcctg gctcaggatg
     2881 cgatgacgca gctgcgggga gcagtgcttc ggcgtcaccg gatccgtggt ggacggcgga
     2941 gtgcggggtg ccgcgttcat ggtgcgtctc ctggcttgat cgggttggac atcaggcgcg
     3001 agacacggcc atgtcggcgc ctgccgtggc gcgcggggcc ttgttgcccc cgcgtgggcg
     3061 catcaggcgc atgccgttca ggatcaccag cagcaccgag gcctgatgaa tgaacatgcc
     3121 gccggccatg tgcacttcgc cggcaaacac gcccgccagc agcccggcca ccgtgagcag
     3181 tgcaatgccg gtgttctgac ggatgttgcg caccgtggcg cgggccagcg tgatggcgcg
     3241 cgccacgcgc tccaggtcat cggtcatgag cgcgatgtcg gccgtgtcga tggccacgtc
     3301 ggtgccggca gcacccatgg ccacgccgac atcggcagcg gccagcgccg gcgcgtcgtt
     3361 gatgccgtcg cccaccatcg ccgtgacgcc ggcctcggtg cgcaagcgct cgatggcttt
     3421 cagcttgtgc tcgggcaaca gtcccgcctg cacctcggtg atgcccaagg tgcgcgccac
     3481 gcgctgggcg ctggccaggt ggtcgccagt gagcatgacg aggcggcgca cgccgccatc
     3541 gcgcagctgc tgcagggccg cggcggcctc ggggcgaacc tggtctgcaa agccgagcaa
     3601 accgaacacc ttgccgtcga cgctgatggc agccagcgtt tcaccgcgct ggcaaatctc
     3661 ctcgagcgcc gcttgcgcat ctgtcggcca gtcggccacg cgttggcgca gccagtcggg
     3721 gctgccgatt tcaatgcggc ggccctgcca gccggcgccg atgccaccgc cggcaaaggt
     3781 ttcgaagttg tcggcggcag cgatcggctc gtcgcccttg acttcgcgca ggatggcgcg
     3841 cgccagcggg tgctcggagc cgccctcggc caccgccgcc cagcgcagca gttgctgctt
     3901 gcggtcgtcc tgcccgatgc cgggcacggg ttgcaaggcc gccagcggaa cgatcgaggt
     3961 caccgccggc cggcccacgg tcagcgtgcc ggtcttgtca aaggccacgg cacggatgcg
     4021 cgcggcggtc tccaggtgct cgccaccctt gatcagaaca ccctggcgcg ccgcctggcc
     4081 gatgccggca atcaccgaaa tgggcgtgga gatgaccagc gcacccgggc aggccacgac
     4141 cagcagcgtc agcgccaact cgatgtcgcc cgtcagcgcg taagtgagga tggtcagcac
     4201 gatgatggcc ggcgtgtacc actgggcaaa gcgctcgatg aagcgctgga tcggcgcctt
     4261 ggcctcctgc gcctgctcga cgcgctggat gatgtgcgcc aacgtggtgt cggcgcccac
     4321 gccctcggcg cgtatctcca ggtagccgtc gtggctgacg gtgccggcaa acaccgagct
     4381 gccttcggac ttgtcctcgg gcatcgattc gccggtgatg gcggcctgat tgacggcggc
     4441 gcgtccgctc agcaccgtgc cgtcgacgcc gatgcgtccg cccgggcgca cgatcacggt
     4501 atcgccgcgc agcacctgat gtggcgccag caccagctcg gtgccgtccc ggcgcaccgt
     4561 gacctcggtg ggcgccaggt cgatcaggtc cttcagcgcg ctgcgcgtct tggcgatggt
     4621 gcgcgcctcc aggtagccgc ccagcaggaa cagaaaggtg acggcggcgg cttcccagta
     4681 ttcaccgatg gcgatggcgc cgacggcggc gatcgtcacc agcagctcga tgctggcact
     4741 gcgcgcgcgc aggctgtgcc aggcgcgcac cgcgatgtcg gagccggcga tgagcgcggc
     4801 cacgatccac agcgtggccg tcagcgcggc cggtccgtcc aggaacgcgc tcagcacgcc
     4861 gcccaggccc agagctccgc tgaccagcgt cagcaggccg cgccgctgcg ctggcgatgc
     4921 gcgcagcgtt tgcagacggg aaacagaatg ggagatggac atgatgaacc tctggaatgc
     4981 agactggaag gggccgggcg cgagcgcgcc cggcagcgac cggacggcgg ttcagaaggc
     5041 gctgatgcgc gagccgtagc cga
//
LOCUS       DAOOOG010000174         5054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_19913_length_5054_cov_20.191438, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000174 DAOOOG010000000
VERSION     DAOOOG010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19913_length_5054_cov_20.191438"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PK242_11180"
     CDS             complement(<1..761)
                     /locus_tag="PK242_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOZ94625.1"
                     /translation="MTPADLFASAQQGLFETLIEPLAFALGLGNLLEDGFVAAGWLLI
                     GVLQIGVLLAVIGPLQRLWPAEPVRDRAAIRVDVLYTLLHRLGLFKLAMFFSVEGWLT
                     DAIGVLRARGLPTLHLDALWPGVTDRPLVSFVLYLLVFDFIGYWVHRAQHQWDWWWKL
                     HAVHHSQRQMTMWSDDRNHLLDSVLTDAIMVVVAILIGVAPSQFVLLVACTQLLESLQ
                     HANLRWSFGRVGERLLVSPRFHRRHHAIGIGHEARA"
     gene            complement(776..1483)
                     /locus_tag="PK242_11185"
     CDS             complement(776..1483)
                     /locus_tag="PK242_11185"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOZ94626.1"
                     /translation="MYLSFDTGHMGVAALVAEVLRRQQVRVTFFAANERTQEGDGSLS
                     RHWGPWWKARAAEGHELASHTWDHVYWRADLPGREPRFRVRPSAGPLAGREFTWSAEQ
                     YCQNIEQASERLAYYSGRKPLPLFRAPGGKISPRLLAAARACGYEHVGWSPAGFLGDE
                     LPSDKYPNRLLLERALKTIQPGDILLAHLGIWSRQDPWAPAVLEPLIEGLKARGFVFR
                     TLREHPQYAPWIAQQRA"
     gene            complement(1590..2546)
                     /locus_tag="PK242_11190"
     CDS             complement(1590..2546)
                     /locus_tag="PK242_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOZ94627.1"
                     /translation="MGAAALAPGALAAPTAAPVLVLNSLDGTVSVIDGRRWVELVRIP
                     TGKEPHHLYMTPDERSVIVANAGSDSLTFIDPRTAIVQRTLRDILDPYHLRFSPDMRW
                     FVTAANRLNHIDLYHWDGQQPHLARRIPTGRTPSHLWIDARSTTVWATMQDSHELVAI
                     DLASQQLMHRVLTGPLPADVFVTPDERHLLLGLTGGSGVEVFELAAGQAPRRVGLVAT
                     GKGAHAFRALGDRRHVLVSNRVAGSISQIDTTRLAVVAEFPAPAGPDCMDVSADGSLI
                     MVGSRWAGKLSLIDVAQRKLVHQVKVGKSPHGVWTLDHAPRV"
     gene            2784..3572
                     /locus_tag="PK242_11195"
     CDS             2784..3572
                     /locus_tag="PK242_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ94628.1"
                     /translation="MPAMRSVDIDGVSLELQHIAPPAGAAPARAPLVFLHEGLGSVAL
                     WRDWPARLCAATGRAGWVYSRRGYGRSSPVPDVRGAGRLPPDYLHREALDVLPALLRA
                     LGLARPVLVGHSDGASIALIHAAHHPVTACVAMAPHVMVEALCLRAIEQTRQHFETGA
                     LRIRLQRFHDDVDGAFWQWNDAWLNPQFHGFDIRADIRSIDAPLLAIQGVDDGYASMA
                     QLDDIATALPHTRLLKLPACGHAPHQDQPAAVQQAITDLLADLP"
     gene            complement(3633..4361)
                     /locus_tag="PK242_11200"
     CDS             complement(3633..4361)
                     /locus_tag="PK242_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOZ94629.1"
                     /translation="MRLSELLFSQGFGTRRVCAGLVEQGLVRLGERRCTDPSAEVDLS
                     AGPLRYEVQGQAWTYHPRAYLMLHKPAGFECSHRPGRWPSVYTLLPAPLRQRPTAGKV
                     AGVQAVGRLDQDTTGLLLLSDDGAFIHRMNAPRRHVPKVYEVGTRHPLDERQLERLRS
                     GVVLDDDPRPVVAAAAEASGERQLRLTLTEGKYHQVKRMLAAVGNRVETLHRSRIGGL
                     SLPAELAPGQWRWLEPSELALLRP"
     gene            4533..>5054
                     /locus_tag="PK242_11205"
     CDS             4533..>5054
                     /locus_tag="PK242_11205"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HOZ94630.1"
                     /translation="MSAISGSTTFWAIVLAMVGLFTGYLGLSSFMPRGRDGQAGKPRS
                     KLRMPQQWPLVPRPLANSRERLVWHWLRKVFPGHHIMVKLPVTRFTMPRQTGEASEWF
                     ELLSRAYCSFTLCDSKGHVIGCVDVLGPHGLSRSNRQLKQTLLAQCGIAYWVISPDAL
                     PEPSALRADFLGVN"
BASE COUNT          768 a         1796 c         1752 g          738 t
ORIGIN      
        1 gctctggcct cgtggccgat gccgatggcg tggtggcggc ggtgaaagcg cgggctgacc
       61 agcaggcgct cgcccacgcg gccgaacgac cagcgcaggt tggcgtgctg caggctttcg
      121 agcagctgcg tgcacgccac gagcagcacg aactgcgacg gcgccacgcc gatcaggatg
      181 gcgaccacga ccatgatggc gtcggtcagc acgctgtcca gcaggtggtt gcggtcgtcg
      241 ctccacatcg tcatctggcg ctgcgaatgg tgcacggcgt gcagcttcca ccaccagtcc
      301 cactggtgct gggcgcggtg cacccagtag ccgatgaagt cgaacaccag caggtacagc
      361 acgaagctga ccagcggccg gtcggtcacg cccggccaaa gcgcgtccag gtgcagcgtc
      421 ggcaggccgc gcgcgcgcag cacgccgatg gcgtcggtca gccagccctc gacgctgaag
      481 aacatcgcca gcttgaacag ccccagccgg tgcagcaggg tgtagagcac gtcgacgcgg
      541 atggccgcgc gatcgcgcac cggctcggcc ggccacaggc gctgcagcgg gccgatcacg
      601 gccagcagca cgccgatctg cagcacgccg atcagcagcc agccggcggc cacgaagccg
      661 tcttccagca ggttgcccag gcccagggcg aaggcgagcg gctcgatcag ggtctcgaac
      721 agcccctgct gggcgctggc gaacaggtcg gcgggcgtca tgctgcgggt accgcctatg
      781 cgcgctgctg cgcgatccag ggcgcgtact gcgggtgctc gcgcagcgtg cggaacacga
      841 agccgcgcgc cttcagcccc tcgatcagcg gctccagcac cgccggcgcc cacgggtcct
      901 ggcgcgacca gatgcccagg tgcgccagca ggatgtcgcc cggctggatg gtcttgagcg
      961 cacgctcgag cagcagtcgg ttggggtatt tgtcgctcgg cagttcatcg cccagaaagc
     1021 cggccggcga ccagcccacg tgctcgtagc cgcaggcgcg tgcggcggcc agcaggcgcg
     1081 gcgagatctt gccgcccggc gcgcgaaaca gcggcagggg ttttctgccg ctgtagtagg
     1141 ccaggcgctc gctggcctgc tcgatgttct ggcagtactg ctcggcgctc caggtgaatt
     1201 cgcgcccggc cagggggccg gccgaggggc gcacgcgaaa gcgcggctcg cgcccgggca
     1261 ggtcggcgcg ccagtacacg tggtcccagg tgtgcgaggc cagctcgtgc ccttcggcgg
     1321 cgcgcgcctt ccaccacgga ccccaatggc ggctcaggct gccgtcgccc tcctgcgtac
     1381 gttcgttggc ggcaaagaac gtcacgcgca cctgctgacg gcgcagcacc tcggccacca
     1441 gcgccgccac gcccatgtgg ccggtgtcga agctcaggta cagcggggcg ctgccagatg
     1501 cagggccatt ttggcctctg gcgcttgtcc agagcgcgcc ggcagctatc gaaaaaagag
     1561 cattgcggcg ttgcacccga ggcctccgct cacacccttg gggcatggtc cagggtccac
     1621 acgccgtgcg gcgacttgcc caccttgacc tggtgcacca gcttgcgctg cgccacgtcg
     1681 atcaggctca gcttgccggc ccagcgcgag ccgaccatga tcagcgaacc gtcggccgac
     1741 acgtccatgc agtccgggcc ggccggcgcc gggaattcgg ccaccaccgc cagccgcgtg
     1801 gtgtcgatct ggctgatgct gcccgccacg cggttgctca ccagcacgtg gcggcggtcg
     1861 cccagcgcgc gaaacgcgtg cgcgcccttg ccggtggcca ccaggccgac gcgccgcggc
     1921 gcctggccgg cggccagctc gaacacctcg accccgctgc cgccggtcag gcccagcagc
     1981 aggtgccgct cgtcgggcgt gacgaacacg tcggccggca gcggcccggt gagcacgcgg
     2041 tgcatcagtt gctgcgacgc caggtcgatc gccaccagct cgtggctgtc ctgcatggtg
     2101 gcccacacgg tggtgctgcg cgcgtcgatc cacaggtggc tgggcgtgcg cccggtcggg
     2161 atgcgccgcg ccaggtgcgg ctgctgcccg tcccagtggt agaggtcgat gtggttgagc
     2221 cggttggccg ccgtcacgaa ccagcgcatg tcgggcgaaa agcgcaggtg gtaggggtcg
     2281 aggatgtcgc gcagcgtgcg ctgcacgatg gccgtgcgcg ggtcgatgaa ggtgagcgaa
     2341 tcgctgccgg cgttggcgac gatgaccgac cgctcgtccg gcgtcatgta caggtggtgc
     2401 ggctccttgc cggtcgggat gcgcaccagc tcgacccagc gccggccgtc gatcacgctc
     2461 accgtgccgt cgagcgagtt gagcaccagc accggcgctg cggtcggcgc cgccagcgca
     2521 ccgggcgcca gcgccgccgc gcccaggccc actccggcgg ccagcagccg gcgacgcgtc
     2581 ggggaaggaa tgtcgatcga tgacaagacg gacctgtgga aaaaaagcgt gggcggtggc
     2641 gcgcgcgagt gtaaacccgc acccgcccca ttcacgatgg attcgaccaa tggtgtctgg
     2701 aatgccgtgc gggctgaact cgtccaagcc cttcgacagg ctcagggtga acggttgaaa
     2761 cttcaagctt ccgaagcaat aacatgccag ccatgcgatc ggtggacatc gacggcgtca
     2821 gcctggagct tcagcacatc gcgccgccgg ccggtgcggc gccggcccgg gcaccgctgg
     2881 tgtttctgca cgagggcctg ggctcggtgg cgctgtggcg cgactggccg gcgcgcctgt
     2941 gcgccgccac cggtcgcgcc ggctgggtgt attcgcgtcg cggctatggg cgctccagcc
     3001 cggtgcccga cgtgcgcggc gccggccgcc tgccgcccga ctacctgcac cgtgaggcgc
     3061 tcgacgtgct gccggccctg ctgcgagcgc tgggcctggc gcgcccggtg ctggtgggcc
     3121 attccgacgg cgccagcatc gccctgatcc acgccgccca ccacccggtc acggcctgcg
     3181 tggcgatggc gccgcacgtg atggtggaag cgctgtgcct gcgcgccatc gagcagacgc
     3241 gccagcactt cgagaccggc gccctgcgca tccgcctgca acgctttcat gacgatgtgg
     3301 atggcgcctt ctggcagtgg aacgacgcct ggctgaaccc gcaattccac ggcttcgaca
     3361 tccgcgccga catccgcagc atcgacgcgc cactgctggc gatccagggc gtggacgacg
     3421 gctacgccag catggcccag ctcgacgaca tcgccacggc gctgccgcac acgcgtctgc
     3481 tgaagctgcc ggcctgcggg cacgcgccgc accaggacca gccggcggcc gtccagcagg
     3541 ccatcaccga ccttctggcc gatctgccct gatgcgcggc ggcgcgtcca agcccatcga
     3601 tcacggctcg ttcgctggca ggcgtcgccg cgtcaggggc gcagcaaggc cagctcggaa
     3661 ggctccagcc agcgccactg ccccggcgcc agctcggccg gcaatgacag gccgccgatg
     3721 cgcgagcggt gcagcgtttc gacccggttg ccgacggcgg ccagcatgcg cttgacctgg
     3781 tggtacttgc cctcggtgag cgtcagccgc agttgacgtt cgccgctcgc ctcggccgcc
     3841 gccgccacca ccgggcgcgg gtcatcgtcc agcaccacgc cgctgcgcag gcgctccagc
     3901 tggcgttcat ccagggggtg ccgggtgccc acctcgtaga ccttgggcac atgccgacgt
     3961 ggcgcattca tgcggtggat gaaggcgccg tcgtcgctca gcagcaacag gccggtggtg
     4021 tcctggtcca gccttccgac cgcctgcacc ccggccacct tgcccgccgt gggccgctgg
     4081 cgcagcggcg ccggcagcag tgtgtagacg ctgggccagc gcccggggcg gtgcgagcac
     4141 tcgaagcctg ccggcttgtg cagcatcagg taggcccgcg ggtggtaggt ccaggcctgg
     4201 ccctgaacct cgtagcgcaa cgggccggcc gacaggtcca cctcggccga cggatcggtg
     4261 caccgccgct cgcccaggcg caccagcccc tgctcgacca ggccggcgca gacccggcgc
     4321 gtgccgaagc cctggctgaa cagcagttcg ctcaggcgca tgcatgactt ccatcaaact
     4381 gtgatgaatc gagagttggc atgacttatt gctcagaaat ccgggacccg gactgaaatt
     4441 agacaaaaag taccttctta ttcgggcggc gcgtgggtaa catcatgcaa cgaaatgccg
     4501 cgccatccgc gcgccacagg gggaacaaga ttttgagcgc catctccggt tcgaccacct
     4561 tctgggccat cgtcctggcc atggtgggtc tgttcaccgg ctacctcggc ctgagcagct
     4621 tcatgccgcg cggccgcgat ggacaggcgg gcaagccccg cagcaagctg cgcatgccgc
     4681 agcagtggcc gctggtcccc agaccgctgg ccaacagccg cgagcgcctg gtgtggcact
     4741 ggttgcgcaa ggtcttcccc gggcatcaca tcatggtcaa gctgccggtc acgcgcttca
     4801 ccatgccgcg ccagacgggc gaggccagcg aatggttcga gctgctcagc cgcgcctatt
     4861 gcagcttcac cctgtgcgac agcaagggcc atgttatcgg ctgcgtggac gtgctggggc
     4921 cccacggact gtcgcgcagc aaccgccagc tcaagcagac cctgctggcc caatgcggca
     4981 tcgcctactg ggtgatctcg cccgatgcgc tgcccgagcc gtcggcgctg cgggccgact
     5041 tcctgggcgt caac
//
LOCUS       DAOOOG010000175         5038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_20009_length_5038_cov_20.671483, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000175 DAOOOG010000000
VERSION     DAOOOG010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20009_length_5038_cov_20.671483"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /gene="rplT"
                     /locus_tag="PK242_11210"
     CDS             <1..305
                     /gene="rplT"
                     /locus_tag="PK242_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOZ94631.1"
                     /translation="ALAKGFRGRRKNVFRVAKQAVMKAGQYAYRDRRNKKRDFRRLWI
                     ARINAAARECGITYSQFINGLSKAGIALDRKVLADIAVHDKAAFAGIVEQAKAKLA"
     gene            425..1492
                     /gene="pheS"
                     /locus_tag="PK242_11215"
     CDS             425..1492
                     /gene="pheS"
                     /locus_tag="PK242_11215"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOZ94632.1"
                     /translation="MTTTATEDLQELVRSADSSFARARTPAELENAKAQFLGKSGRVT
                     ELMKGMGALSVDEKKTRGAAINQAKQAIEALLTDRRQALADAELARQLQAEALDVTLP
                     GRQRGMGALHPISLAWERIEQIFGSMGFDVGDGPEIENDWFNFTALNNPPNHPARSMQ
                     DTFYVDMSDEQGQPYCLRTHTSPMQIRYATAHARRHAAALAAGQIPEVRVIVPGRTYR
                     VDNDATHSPMFHQVEGLWLGENVSFKDLKVIYLNFCKAFFETEDLILRFRPSYFPFTE
                     PSAEIDIQFQTGPLAGRWLEVSGSGQVHPQVVRNMGLDPERFIGFAFGSGIDRLAMLR
                     YGVNDLRLFFEGDIRFLSQFR"
     gene            1551..3986
                     /gene="pheT"
                     /locus_tag="PK242_11220"
     CDS             1551..3986
                     /gene="pheT"
                     /locus_tag="PK242_11220"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOZ94633.1"
                     /translation="MQFPESWLREFCNPPLSTEQLADTLTMAGFEVEELRPVAPPFTR
                     VVVAEIRSAEPHPNADRLRVCQVDAGQGTLLNIVCGAPNARAGIKVPCALVGAELPPG
                     EDGKPFLIKTGKLRGVESQGMLCSARELQLSDDHGGLLELPADTPVGQDIRAALRLDD
                     AVFTLKLTPNLGHALSVYGIARELSALTGAPLRTPTLPPVTVGCDQVLPVRVSAPDLC
                     GRFSGRVIRHVNTRAATPPWMVDRLARCGQRSVTALVDISNYVMFEYGRPTHIFDLDR
                     IHGGLDVRWGRAGETLKLLNGNTIEVDETVGVIADEREVESLAGIMGGDATAVSDDTQ
                     HVYVEAAFWWPDAVAGRSRRFNFATDAGHRFERGVDPALTVAHIERISQLIVEICGTP
                     QTVCGPIDDQQPQLPVPAPVTLRVARAAQIIGMPLTAAQCADVFGRLGLPFTEGQGTI
                     TVVPPSYRFDLRIEEDLIEEVARVIGYQQLPDTAPLAPIVPRVPSATRRGAFAVRRAL
                     AGLGYLETINFSFVDESWEHQLCGNPDPVRLLNPIASQMSVMRSSLLGSLLQVIKHNV
                     DRRAERVRVFELGRVFRRDATVATSDADVRGVAQPMHVAAAALGPTSRQGWQGRQGAV
                     DFYDVKGDVEALLSPRQPTFEPAEHPALHPGRSARVLLDGRPIGVIGELHPRWRQQWE
                     LPQAPILFELALDAVMQQALPQAQPVPRQQPVERDIAVVVPESVTHAALTQAIHAAPT
                     EGLLRSATLFDIYRPKPGAEGQAGMASHEKSLAVRLVLGSTHDTLTDERIDAAVKAVL
                     ERLATELGARLRA"
     gene            4040..4405
                     /locus_tag="PK242_11225"
     CDS             4040..4405
                     /locus_tag="PK242_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HOZ94634.1"
                     /translation="MLLAVDSLETSALTKAQLAELLFEQIGLNKREAKDMVDAFFELI
                     VQHLIAGDEVKLSGFGNFQMRVKAPRPGRNPRTGELIPIAARRVVTFHASQKLKDQLQ
                     AEAGSPAPLQVGAEPDQAA"
     gene            4509..4979
                     /locus_tag="PK242_11230"
     CDS             4509..4979
                     /locus_tag="PK242_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOZ94635.1"
                     /translation="MDKTLPPIPAKRYFTIGEVGELCGVKPHVLRYWEQEFTQLRPMK
                     RRGNRRYYQHHEVLMIRRIRELLYDQGFTINGARVKLQELTQHERDRARAGELALHGL
                     DRFDADETVPSEFGDSLSFEDSQLMPEQIREVERELKEIRDLLTVSQWHDLHAL"
BASE COUNT          842 a         1749 c         1649 g          798 t
ORIGIN      
        1 tggccctggc caagggtttc cgcggccgcc gcaagaacgt cttccgcgtc gccaaacagg
       61 cggtgatgaa ggccgggcag tacgcctacc gtgaccgccg caacaagaag cgcgatttcc
      121 gccgcctctg gattgcccgc atcaacgccg ccgcgcgtga atgcggcatc acctacagcc
      181 agttcatcaa cggcctgagc aaggccggca ttgcgctgga tcgcaaggtg ctggccgaca
      241 tcgccgtgca cgacaaggcc gcctttgccg gcatcgtgga gcaggccaag gccaagctgg
      301 cctgattccg acgccggtgc agcaactata cttttgatag ctgctcgcgc tggcggttca
      361 aggggttgag gcctttttgg cttcaatccc tttttcattt ccgtttcact gcccgattgt
      421 ttcgatgacc accaccgcca ccgaggacct gcaggaactc gtccgcagcg ccgacagcag
      481 ctttgcccgc gcccgtacgc cggccgaact cgaaaacgcc aaggcccagt ttcttggcaa
      541 gtcggggcgc gtgaccgaac tgatgaaggg catgggtgcg ctgagcgtcg acgagaagaa
      601 gacccgcggt gccgccatca accaggccaa gcaggccatc gaagcgttgc tgaccgatcg
      661 ccgtcaggcg ctggccgacg ccgagctggc gcgccagctg caggccgagg cgctggacgt
      721 gacgctgccc ggccgccagc ggggcatggg cgcgctgcac cccatcagcc tggcctggga
      781 gcgcatcgag cagatcttcg gcagcatggg cttcgacgtc ggcgacgggc cggagatcga
      841 gaacgactgg ttcaacttca ccgccctgaa caacccgccc aaccaccccg cgcgctcgat
      901 gcaggacacc ttctacgtcg acatgagcga cgagcagggt cagccctact gcctgcgcac
      961 ccacaccagc ccgatgcaga tccgctacgc cacggcgcac gcgcgccggc acgccgcagc
     1021 gctggcggcg ggccagatcc ccgaagtgcg cgtgatcgtg cctggccgca cctatcgggt
     1081 cgacaacgat gccacccatt cgcccatgtt ccaccaggtc gaaggcctgt ggctggggga
     1141 gaacgtcagc ttcaaggacc tgaaggtcat ctacctcaac ttctgcaagg ccttcttcga
     1201 aaccgaagat ctgatcctgc gttttcggcc cagctatttc ccgttcaccg agccgagcgc
     1261 cgagatcgac atccagttcc agaccggccc gctggcgggc cgctggctgg aagtgtccgg
     1321 ctccggccag gtgcacccgc aggtggtgcg caacatgggg ctggatccgg agcgcttcat
     1381 cggctttgcc ttcggctccg gcatcgatcg tctggccatg ctgcgctacg gcgtcaatga
     1441 cctgcgcctg ttcttcgaag gcgacatccg cttcctgagc cagttccgct gattcaggcg
     1501 cagcctcgtt caccggccca cgtcccacct gctcacccct ccaaagaaaa atgcagtttc
     1561 ccgaatcctg gttgcgcgag ttctgcaacc cgcctctttc caccgagcag ctggccgaca
     1621 ccctgaccat ggccggcttc gaggtggagg agctgcgccc cgtggcgccg ccgttcacgc
     1681 gcgtggtggt ggccgaaatc cgcagcgccg agccgcaccc caacgccgac cgcctgcgcg
     1741 tgtgccaggt cgacgccggg cagggcacgc tgctgaacat cgtgtgcggc gcgcccaacg
     1801 cgcgcgccgg catcaaggtg ccctgtgccc tggtgggcgc cgagctgccg ccgggcgagg
     1861 acggcaagcc ttttctcatc aagaccggca agctgcgcgg cgtcgagagc cagggcatgc
     1921 tgtgttcggc gcgtgagctg cagctgtcgg acgaccatgg cggcctgctg gagctgcccg
     1981 ccgacacgcc ggtggggcag gacatccgcg ccgcgctgcg gctcgacgac gccgtgttca
     2041 cgctcaagct cacccccaac ctgggccacg ccctgagcgt gtacggcatc gcgcgcgaac
     2101 tgtcggccct gacgggcgcg cccttgcgca cgccgacgct gccgccggtg accgtgggct
     2161 gcgatcaggt gttgccggtg cgcgtcagcg cgcccgacct gtgcggccgc ttttccggcc
     2221 gcgtgatccg ccacgtcaac actcgcgccg ccacgccgcc gtggatggtc gatcggctgg
     2281 cgcgctgcgg tcagcgcagc gtcaccgcgc tggtggacat ctcgaactac gtgatgttcg
     2341 agtacggacg gcccacccac atcttcgatc tggaccggat ccacggcggc ctggacgtgc
     2401 gttggggccg tgccggcgaa accctgaaac tgctcaacgg caacaccatc gaggtggacg
     2461 aaaccgtcgg tgtgatcgct gacgagcgcg aggtcgagtc gctggcgggc atcatgggcg
     2521 gcgacgccac cgcggtctcc gacgacacgc agcatgtcta cgtcgaggcc gccttctggt
     2581 ggcccgacgc cgtggccggg cgttcgcgcc gcttcaactt cgccaccgat gccgggcacc
     2641 gtttcgaacg tggggtcgac ccggccctga cggtggcgca catcgaacgc atcagccagc
     2701 tcatcgtcga gatctgcggt acgccgcaga ccgtgtgcgg cccgatcgac gaccagcagc
     2761 cccagctgcc ggtgccggcg ccggtcacgc tgcgggtggc gcgcgcggcg cagatcatcg
     2821 gcatgccgct gacggccgca cagtgcgccg acgtgttcgg tcgtctgggc ctgccgttca
     2881 ccgaagggca gggcacgatc accgtggtgc cgccttccta ccggttcgac ctgcgcatcg
     2941 aggaagacct gatcgaggaa gtggcgcgcg tgatcggcta ccagcaactg ccggacaccg
     3001 cgcctctggc gccgatcgtg ccgcgcgtgc cctcggccac ccgacgcggc gcctttgccg
     3061 tgcgccgcgc gctggcgggt ctgggttacc tggaaaccat caacttcagc ttcgtcgacg
     3121 aaagctggga gcaccagctg tgcggcaatc ccgatccggt gcgcctgctc aaccccatcg
     3181 ccagccagat gagcgtgatg cgctcatcgc tgctggggtc gctgctgcag gtcatcaagc
     3241 acaacgtcga ccggcgcgcc gagcgcgtgc gtgtgttcga gctggggcgc gtgttccggc
     3301 gtgacgccac ggtggccacc agcgacgccg acgtgcgcgg cgtggcccag cccatgcacg
     3361 tggcggcagc cgcgttgggg cccaccagcc gccagggttg gcagggccgg cagggagcgg
     3421 tggatttcta cgacgtcaag ggtgatgtcg aagccctgct cagcccgcgc cagcccacgt
     3481 tcgagcccgc cgagcatccg gccctgcacc cggggcgcag cgcgcgcgtg ctgctggacg
     3541 ggcggccgat cggcgtcatc ggcgagctgc acccacgctg gcgccagcag tgggagctgc
     3601 cgcaggcgcc gatcctgttc gagctggcgc tcgacgccgt gatgcagcag gcgctgcccc
     3661 aggcgcagcc cgtgccgcgc cagcagccgg tggagcgcga catcgccgtg gtggtgcccg
     3721 aatcggtcac ccatgcggct ctgacgcagg ccatccatgc tgcccccacc gagggcctgc
     3781 tgcgcagcgc cacgctgttc gacatctatc gcccgaaacc gggcgcggaa gggcaggccg
     3841 gcatggccag ccatgaaaaa agcctggccg tgcgcctggt gctgggcagc acacacgaca
     3901 cgctgaccga cgagcgcatc gacgccgccg tgaaggcggt gctggagcgg ctggcgaccg
     3961 agcttggcgc caggctgcgc gcctgatgct caccgatgga gagacgccca tggccgctca
     4021 cgatccgacc gaagacgcca tgctgctggc cgtcgacagc ctggagacct cagccttgac
     4081 caaggctcag ctggcggaac tgctgttcga gcagatcggc ctgaacaagc gcgaagccaa
     4141 ggacatggtc gacgcatttt tcgaactcat cgtgcagcac ctgatcgcgg gcgacgaggt
     4201 caagctgtcg ggatttggca atttccagat gcgcgtcaag gcgccacgtc cgggccgcaa
     4261 cccgcgcacc ggcgaactca ttcccatcgc cgcccgtcgg gttgtcacgt ttcacgccag
     4321 ccagaagctc aaggaccagc tgcaggccga ggccgggtcc ccggcaccgc tgcaggttgg
     4381 ggccgaaccc gaccaggccg cctgaccccc tgtttcaggg ggtgtcgggg tggggtgtcg
     4441 ctggcctgta gccgcgacga acgagttgcg gtaacctcac tggttcagca cgcaagtgcc
     4501 tgatccgcat ggacaagacc ctgcctccga ttcctgccaa gcgctacttc accatcggcg
     4561 aagtcggcga gctgtgcggc gtcaaaccgc atgtgctgcg ctattgggag caggaattca
     4621 cccaactgcg gcccatgaag cgccgtggca accgacgcta ctaccagcac cacgaggtgc
     4681 tgatgatccg gcgcatccgc gagctgctgt atgaccaggg cttcaccatc aatggcgcgc
     4741 gggtgaaact gcaggaattg acccagcacg agcgcgaccg tgcccgggcc ggcgaactgg
     4801 ctctgcacgg tctcgaccgt ttcgacgccg acgaaaccgt gcccagtgag tttggtgaca
     4861 gcctgtcgtt cgaggactcc cagctcatgc ccgaacagat ccgcgaagtc gaacgcgaac
     4921 tgaaggaaat ccgcgatctg ctcaccgtct cgcagtggca cgatttgcac gcgctataat
     4981 gcaaggcttg tcggcgtgta gcgcagcctg gtagcgcact tgcatggggt gcaagggg
//
LOCUS       DAOOOG010000176         5029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_20056_length_5029_cov_28.653197, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000176 DAOOOG010000000
VERSION     DAOOOG010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20056_length_5029_cov_28.653197"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK242_11235"
     CDS             <1..531
                     /locus_tag="PK242_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOZ94636.1"
                     /translation="FQTFSLAYTARVLGQLKRTGADGQVVPRIVFTKGGGLWLSDMAA
                     LDCDVLGLDWTVNLGTARRMLTQIAQNATTLIAAGACPASAGGQNDATSRHPKALQGN
                     IDPNVLFAPPEAIRAEVRRVLDSFGRPHGDPAVPGSTHIFNLGHGISQHTPPEHVAHL
                     VDEVHRHSRLLRARSA"
     gene            932..3052
                     /gene="priA"
                     /locus_tag="PK242_11240"
     CDS             932..3052
                     /gene="priA"
                     /locus_tag="PK242_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOZ94637.1"
                     /translation="MTHWLAVAVATPAHSGLGEPLTYRADAPLAPGSIVRVPLGAREV
                     LGVAWGAAATPAPDWAAQVRPLAGVFEGLTPLSDDWRRLIAFTASYYQRALGEVALAA
                     LPPQLRTLSSAQMARRLRRLGEAPPAGAAAAAAPTALTAEQAAALARIAQEPGPFLLH
                     GATGSGKTEVYMQAAARVLAHQPAAQVLVLVPEINLTPQLEARFAARFGAAACVSLHS
                     GLTPPQRLAAWLAAHSGRARIVLGTRVAVLASMPQLALIVVDEEHDPSYKQQEGARYS
                     ARDLALMRGRLAGAQVILGSATPSLESWHASAPAPEGPGRLRRLAMPRRVGLTPADDP
                     ATGLPRVRRVDMSRQPRGTLIAAPLLAAIGERVARGEQALLLLNRRGWAPVLHCADCG
                     WKSECPHCSAYRVFHRIDRSLRCHHCGFTQPVPRACPACGNLDLGSLGRGTEQLEAQL
                     AGLLADVRRPDGAPARIARIDADSTRGKGQLDSALAQVHAGDIDVLVGTQMIAKGHDF
                     RRVTLVAAIQPDAALFSSDFRAPERLFALLMQAAGRAGRDAASAAGSEMWVQTHHPQH
                     PLFEALKAHDYPGFAARQLAERAQAGLPPFSRLALLRAEARTQAAAQAFLAATAQAAQ
                     QPAHPDPQLAEALAQVTWYPAVPLAVQRVAGIERAQMLLESPSRAALQRVLAACPPLL
                     HQLRTAPAHRGIVRWAIDVDPLAL"
     gene            3097..4008
                     /locus_tag="PK242_11245"
     CDS             3097..4008
                     /locus_tag="PK242_11245"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HOZ94638.1"
                     /translation="MTPPPHPRRRAWLCRAGLALVLSAGGLPAGAWNARGHAAIAALA
                     EAGLNDAARAQVQALLAQDLDRFGRPSGRRTLAAVASWPDEIRAEAARRDPEAYRGWH
                     VRANPVCGERLGRCPQGHCVDQLIIHHTAVLRDHTQPLRQRNEALKWVVHLVGDLHQP
                     LHSGVNPNGGRARVELVGATPPADASLHAVWDGALLDAALAGWQPRTPAADTAALALD
                     APTGWMLETRDVALTQVYQPLNGFACGIRLAEPLRLDADYQRASVPVIRQQIERAARR
                     LTQLLHQALAAQGAEALTDAAPPPPKN"
     gene            complement(3974..4948)
                     /locus_tag="PK242_11250"
     CDS             complement(3974..4948)
                     /locus_tag="PK242_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOZ94639.1"
                     /translation="MHALAGIFSVTFPFFALVLCGYLAARSRLLPLDAVPGLNMFVLY
                     FALPCMLWRFGSGTPLLQLFHPLIALLWLAAAALIVAGTAWLARRRGGGWPDAAMGAL
                     VAAFPNSGFMGVPLILALLGEAAVGPVMATLLVDLVVTTSVCIGLSQWGAADEHGAAK
                     AAGRALQGVLRNPMPWSILLGALTGALGLAPWAPLARTVDLLADAASPVALFTIGAVL
                     ARSQVQAQTRAQSGAAGLGDVGVITLVKLLVHPLLVWALGWLAVRAGGLTPATVTSLV
                     LTAALPAAGNVSLLAERFGADNGRVARIILWSTALAFFSFSGVVALRP"
BASE COUNT          754 a         1949 c         1739 g          587 t
ORIGIN      
        1 ttccagacct tcagcctggc ctacaccgcg cgcgtgctgg gccagctcaa gcgcaccggc
       61 gccgacggtc aggtggtgcc gcgcatcgtc ttcaccaagg gcggcggact gtggctgtcc
      121 gacatggcgg cactggactg cgacgtgctg ggcctggact ggaccgtgaa cctgggcacg
      181 gcgcgccgca tgctcacgca gattgcccag aatgctacaa ctcttatagc tgctggcgct
      241 tgcccagcaa gcgccggagg ccaaaacgat gccacatccc ggcaccccaa ggcgctgcag
      301 ggcaacatcg accccaacgt gctgttcgca ccgccggagg cgatccgcgc cgaagtgcgg
      361 cgcgtgctgg acagcttcgg ccgcccgcac ggcgaccccg ccgtgccggg cagcacccac
      421 atcttcaacc tgggccacgg catcagccag cacacgccgc ccgagcatgt ggcgcacctg
      481 gtcgatgagg tgcaccgcca ttcgcgcctg ctgcgggcgc ggtcggcctg accgccgctg
      541 cgccgccacg gccacgggca cccgcctact gctgcggccg ccccgtgtca agcgcgcagc
      601 gggcatggtt cacacttcat ccagcaactc gggggcttga cttatgcaca aaaaccggcg
      661 tgcacccgct tcggaaggcc cggcccacga ccccgcgcta caaaacgcga agcgcagcta
      721 agttattgat ttgattggac ttcaagtgtt gctgctttgg caggcaggat aaggaaaccc
      781 ttgattttca aggcctgcgg gcgagcgccg gggaattctc aacaaagtta tccacatatt
      841 ctgggcatga cttggcagac gctttcaaaa caagcactta agcccgaaac tgcaagtgtc
      901 ggcaagccat gacccagatc aggttcaccg catgacgcac tggctggccg tcgccgtcgc
      961 caccccggca cacagcggcc tgggcgagcc gctgacctac cgcgccgacg caccgctggc
     1021 gcccggcagc atcgtgcgcg tgccgctggg tgcgcgcgag gtgctgggcg tggcgtgggg
     1081 cgcagcggcc acccccgcgc ccgactgggc ggcccaggtg cgtccgctgg ccggcgtgtt
     1141 cgagggcctg acgccgctgt ccgacgactg gcgccggctg atcgccttca ccgccagcta
     1201 ctaccagcgc gccctgggcg aagtggcgct ggcggcgctg ccgccgcagc tgcgcacgct
     1261 cagcagcgcc cagatggcgc gccgcctcag gcgcctgggc gaggcgccgc cagccggcgc
     1321 cgccgccgca gcagcgccaa cggcgctgac ggcggagcag gcggccgccc tggcccgcat
     1381 cgcgcaggag ccgggcccgt ttctgctgca cggcgccacc ggcagcggca agaccgaggt
     1441 ctatatgcag gccgccgcgc gcgtgctggc gcaccagccg gcggcgcagg tgctggtgct
     1501 ggtgcccgag atcaacctga cgccgcagct ggaagcgcgc tttgccgccc gcttcggcgc
     1561 cgccgcctgc gtgtcgctgc acagcggcct gacgccgccg cagcgcctgg cggcctggct
     1621 ggcggcgcac agcgggcgcg cccgcatcgt gctgggcacg cgggtggcgg tgctggcgtc
     1681 gatgccgcag ctggcgctga tcgtggtgga cgaagagcac gatcccagct acaagcagca
     1741 ggaaggcgcg cgctactcgg cgcgcgacct ggcgctgatg cgcgggcgcc tggccggcgc
     1801 gcaggtgatc ctgggctcgg ccacgccgtc gctggaaagc tggcacgcca gcgcccccgc
     1861 acccgaaggc cccggccgcc tgcggcgcct ggccatgccg cgccgcgtcg gcctgacgcc
     1921 cgccgacgac ccggccaccg gcctgccgcg cgtgcgccgc gtcgacatgt cgcgccagcc
     1981 caggggcacg ctgatcgccg ccccgctgct ggccgccatc ggcgagcgcg tggcgcgtgg
     2041 cgagcaggcg ctgctgctgc tcaaccgccg cggctgggcg ccggtgctgc actgcgccga
     2101 ctgcggctgg aaaagcgagt gcccgcattg cagcgcctac cgcgtattcc accggatcga
     2161 ccgctcgctg cgctgccacc actgcggctt cacccaaccg gtgccacgcg cctgtcccgc
     2221 gtgcggcaac ctcgacctcg gcagcctggg ccgtggcacc gagcagctcg aagcgcagct
     2281 ggccggtctg ctggccgatg tgcgccgccc cgacggcgcg ccggcgcgca tcgcccgcat
     2341 cgacgccgac agcacgcgcg gcaagggcca gctcgacagc gccctggccc aggtgcacgc
     2401 cggcgacatc gacgtgctgg tgggcacgca gatgatcgcc aaggggcacg acttcaggcg
     2461 cgtcaccctg gtggccgcca tccagccgga cgcggccctg ttttccagcg actttcgcgc
     2521 ccccgagcgg ctgttcgccc tgctcatgca ggccgccggc cgcgccggcc gcgacgccgc
     2581 cagcgccgcc ggcagcgaaa tgtgggtgca gacccaccac ccccagcatc ccctgttcga
     2641 ggcgctgaaa gcccacgact accccggctt cgccgcccgc cagctggccg agcgcgccca
     2701 ggccggcctg ccgccgttca gccgcctggc cctgctgcgc gccgaggcgc gcacacaggc
     2761 ggcggcgcag gcgtttctcg ccgccacggc gcaggccgct cagcagccag cgcaccccga
     2821 cccgcagctg gccgaggcgc tggcgcaggt cacctggtac ccggccgtac ccctggcggt
     2881 gcagcgcgtg gccggcatcg agcgcgccca gatgctgctg gaaagcccct cgcgcgccgc
     2941 cctgcagcgc gtgctggccg cctgcccgcc gctgctgcac cagctgcgca ccgcgcccgc
     3001 gcaccgcggc atcgtgcgct gggcgatcga cgtcgatccg ctggcgttat gagtctccgc
     3061 ctccggaacc atcggttcga cactgcacgg cccgcgatga cgccgccacc ccacccgcga
     3121 cgacgggcct ggctgtgccg cgcagggctg gcgctggtgc tgagcgccgg cggcctgccg
     3181 gccggcgcct ggaatgcgcg cggccatgcg gcgatcgccg cgctggccga agccggcctg
     3241 aacgacgccg cccgcgcaca ggtgcaggcg ctgctggcgc aggacctgga ccgcttcgga
     3301 cggccatcgg ggcgccgcac gctggcggcg gtggcgtcgt ggccggacga gatccgcgcc
     3361 gaagcggccc gccgcgaccc cgaggcctac cgcggctggc acgtgcgcgc caacccggtc
     3421 tgtggcgagc gcctgggccg ctgcccgcag gggcattgcg tcgatcaact gatcatccac
     3481 cacaccgccg tgctgcgcga ccacacgcag ccgctgcggc agcgcaacga agcgctgaaa
     3541 tgggtggtgc acctggtggg cgatctgcac cagccgctgc actcgggcgt caaccccaac
     3601 ggcggccgcg cgcgggtgga gctggtcggt gccaccccgc ccgccgacgc cagcctgcac
     3661 gcggtctggg acggtgcgct gctggacgcc gcgctggccg gctggcaacc gcgcacgccc
     3721 gccgccgaca ccgccgcgct ggcgctcgat gcgccgaccg gctggatgct ggagacgcgc
     3781 gacgtggcgc tgacccaggt ctatcagccg ctgaacgggt tcgcctgcgg catccgactg
     3841 gccgaaccgc tgcggctgga cgccgactac cagcgcgcca gcgtgccggt gatccgccag
     3901 cagatcgagc gcgccgcgcg gcggctgacc cagctgctcc atcaggcgct ggcggcgcaa
     3961 ggcgccgagg cgctcacgga cgcagcgcca ccacccccga aaaactgaag aacgccagcg
     4021 ccgtcgacca caggatgatg cgcgccacgc gcccgttgtc ggcgccgaag cgctcggcca
     4081 gcagcgacac gttgccggcc gccggcagcg ccgccgtcag caccagcgag gtcacggtgg
     4141 ccggcgtcag cccgccggca cgcacggcca gccagcccag cgcccacacc agcagcgggt
     4201 gcaccagcag cttgaccagc gtgatcaccc cgacgtcacc caacccggcc gcgccggact
     4261 gggcacgggt ctgcgcctgg acttgcgagc gcgccagcac cgcaccgatg gtgaacagcg
     4321 ccaccggcga agcggcgtcg gccagcagat cgacggtgcg cgccagcggc gcccacggcg
     4381 ccaggcccag cgcaccggtc aacgcgccca gcaggatcga ccatggcatc gggttgcgca
     4441 gcaccccctg caaggcccgc cccgccgcct tggcggcgcc gtgctcgtcg gccgcgcccc
     4501 attgcgacag gccgatgcac accgacgtgg tgaccaccag gtccaccagc agcgtggcca
     4561 tgaccgggcc gacggcggcc tcgcccagca gcgccaggat cagcggcacg cccatgaagc
     4621 cggaattggg aaaggccgcc accagcgcgc ccatggcggc atccggccag ccgcccccgc
     4681 gccggcgcgc cagccaggcg gtgccggcca cgatcagcgc cgccgccgcc agccacagca
     4741 gcgcgatcag agggtgaaac agctgcagca gcggcgtgcc actgccgaag cgccacagca
     4801 tgcaaggcag cgcgaagtac agcacgaaca tgttcagccc cggcacggca tccagcggca
     4861 gcaggcgcga gcgcgccgcc aggtagccgc acagcaccag ggcaaagaag gggaaggtga
     4921 cggaaaagat gccggccagc gcgtgcatgt ggagcgcaag cgtagcaggc cgcggcggct
     4981 atcatcggcg cccccatgtc cgaccgcctg aacctcgccc agcaggacg
//
LOCUS       DAOOOG010000177         5029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_20057_length_5029_cov_23.906916, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000177 DAOOOG010000000
VERSION     DAOOOG010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20057_length_5029_cov_23.906916"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="PK242_11255"
     CDS             complement(<1..158)
                     /locus_tag="PK242_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HOZ94640.1"
                     /translation="MSDPMVERVQRNPKYQQLKQARSRFGWTLAILMLIVYYGYIGLI
                     AFDKEFLAR"
     gene            543..2210
                     /locus_tag="PK242_11260"
     CDS             543..2210
                     /locus_tag="PK242_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ94641.1"
                     /translation="MATATPSKAIRPMTTEERKVIFASSLGTVFEWYDFYLYGSLAAI
                     IAKQFFAGLDPTSAFIFALLAFAAGFIIRPFGALVFGRLGDMIGRKYTFLVTILIMGI
                     STFIVGVLPNYASIGVAAPVILICLRLLQGLALGGEYGGAATYVAEHAPHGKRGAYTS
                     WIQTTATLGLFLSLLVILGTRTIMGEPAFNDWGWRIPFLVSILLLAVSVWIRLSLSES
                     PAFQKMKAEGKTSKAPLSESFGQWKNLKIVLLALFGLTAGQAVVWYTGQFYALFFLTQ
                     QLKVDAVTANLMIAAALVIGTPFFVVFGSLSDKIGRKPIIMAGCLLAILTYFPVFKAL
                     AEAANPDLAAAQAKNKVVVVADPAECSFQFNPTGTVKFTSSCDIAKQVLAAGSVSYDN
                     EAAPAGTPAVIKIGDDVIQSYTTKGIPADEAKAKDAAFKKAVAETLKKDGYPTKADPA
                     KMNKGMMIVILAYLVILVTMVYGPIAAALVEMFPTRIRYTSMSLPYHIGNGWFGGLLP
                     TTSFAIVAQTGNMYNGLWYPIIVAGMTLVIGMLFFRETKDVDIYAED"
     gene            2353..2502
                     /locus_tag="PK242_11265"
     CDS             2353..2502
                     /locus_tag="PK242_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94642.1"
                     /translation="MWKIALGFVVFAAISLYVIMQAGDKVDMSGEKHGADAVHVPDAP
                     ASAAK"
     gene            2726..>5029
                     /locus_tag="PK242_11270"
     CDS             2726..>5029
                     /locus_tag="PK242_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOZ94643.1"
                     /translation="MTDGLIRIRGARQHNLKNLDLDLRTGELTVVTGVSGSGKSSLVF
                     DTLYAEGQRRYVETFSPYARQFLDRMDKPAVDQVEGVPPAIAIDQTNPVRNSRSTVGT
                     MTELNDHLKLLFARAGRLFDRQTALAVRHDTPDSIFAELHRRAGEHGDLRLVATFAVE
                     LPASTTAEDVEQWLSAAGFTRVQAERTEGDKKILDVVADRFRMGGAERARVIEAIELA
                     LLRGGGRMTVYAVPEAGDPEPWKFSTGLHCPESDIRYGDPIPSMFSFNSAVGACPDCR
                     GFGRVIGVDFGLVIPDGRLTLRAGAIKPFQTPAWSECQDDLMRHAEGAGIPRDTPWNK
                     LTPEQQRWVIEGTPSYREGQWSKQWYGVRRFFAYLESKAYKMHIRVLLSKYRSYTPCP
                     SCGGARLKLESLLWRVGSKEDADAVLEPRQRFMPQGVAWTREQLETLPGLCLHDLMLL
                     PIDRLASFFNRLHGASKNGAAGAHQESAEGHFDAPSADAQALKLILDEIQTRLKYLTD
                     VGIGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMDRITQA
                     MQRLRSAGNTLVVVEHDPAVMLAADRVLDFGPGPGAAGGRIVFDGTPGELRGADTLTG
                     AYLGGRKSIGLGFSRLVKADTPRLLLEGVREHNLRGIDVEFPLQRLVTVTGVSGSGKS
                     RLIQDVLAPALMRHFGQSTESPGAHDRLLGAEQLSGVVFVDQSPIGKTARSNPVSYVG
                     AWDAMRKLFADAPLARQRGYTASKFSFN"
BASE COUNT          831 a         1746 c         1613 g          839 t
ORIGIN      
        1 cgggccagga actccttgtc gaacgcgatc aggccgatgt agccgtaata cacgatcagc
       61 atcaggatcg ccagcgtcca gccgaagcgg ctgcgtgctt gcttgagttg ctggtacttg
      121 gggttgcgct ggacgcgctc gaccatcggg tcgctcatgc ctcggtctcc ttgcagggtt
      181 cgcgccgccg gttcggcagg cgcggcgcga ttctgggcag gggcactgac caagtactta
      241 cgctgcgacc gccgtcacat accgttgcaa caacactgcg ggacgctgac gcgttttttt
      301 tgatcggggt tttcaccagt cgatccgccc cgcgcggaga ccgaaatcgg ccctcaattt
      361 cacatcaaaa aacgatgcct cagggttgcc acggagtttg tcagcctgtc ttcagtttcg
      421 cgggactgac cggcgaacgg tcgtcagaaa gcggtcaggc gccgatttca catttgcgcc
      481 caggacacaa ctcgttgcgt gtccgcagac tcgttctcac cgcaattaca ggagacagac
      541 tcatggcgac tgcaaccccg tccaaggcca tccggccgat gacgaccgag gaaaggaagg
      601 tgatcttcgc ctcctcgctc ggcaccgtgt tcgagtggta cgacttctac ctctacggct
      661 cgctggcggc catcatcgcc aagcagttct ttgccgggct cgatccgacc tcggccttca
      721 tcttcgcgct gctggccttt gccgccggct tcatcatccg ccccttcggg gcgctggtgt
      781 tcggccgcct gggcgacatg atcggtcgca agtacacctt cctggtcacc atcctgatca
      841 tgggcatctc gaccttcatc gtcggcgtgc tgcccaacta cgccagcatc ggcgtggccg
      901 cgccggtcat cctgatctgc ctgcgcctgc tgcagggcct ggcactgggt ggcgaatacg
      961 gcggcgctgc cacctacgtg gccgagcacg ccccgcacgg caagcgcggt gcctacacgt
     1021 catggatcca gaccaccgcc acgctgggtt tgttcctgtc gctgctggtg atcctgggca
     1081 cgcgcaccat catgggcgag ccggcgttca acgactgggg ctggcgcatt ccgttcctgg
     1141 tgtcgatcct gctgctggcg gtgtcggtgt ggattcgcct gtcgctgtcc gagtcgcccg
     1201 ccttccagaa gatgaaggcc gagggcaaga cttccaaggc gccgctgtcc gagtccttcg
     1261 gccagtggaa gaacctcaag atcgtgctgc tcgccctgtt cggcctgacc gccggccagg
     1321 ccgtggtgtg gtacacgggc cagttctacg ccctgttttt cctcacgcag cagctcaagg
     1381 tcgacgccgt caccgccaac ctgatgatcg ccgccgcgct ggtgatcggc acgccgttct
     1441 tcgtcgtgtt cggctccctg tccgacaaga ttggtcgcaa gcccatcatc atggcgggct
     1501 gcctgctggc catcctgacc tacttcccgg tgttcaaggc cctggccgaa gccgccaacc
     1561 ccgatctggc ggcggcgcag gccaagaaca aggtggtggt ggtggccgac ccggccgaat
     1621 gctcgttcca gttcaacccg acgggcacgg tcaagttcac cagctcctgc gacatcgcca
     1681 aacaggtgct ggcggctggc tcggtgagct atgacaacga agcggccccc gccggcacgc
     1741 ccgccgtcat caagatcggc gacgacgtga tccagagcta caccaccaag ggcatcccgg
     1801 ccgacgaagc caaggccaag gacgccgcct tcaagaaggc cgtggccgaa accctgaaga
     1861 aggacggcta ccccaccaag gccgaccccg ccaagatgaa caagggcatg atgatcgtca
     1921 tcctggccta cctggtgatc ctggtgacca tggtctacgg cccgatcgcc gccgcgctgg
     1981 tggagatgtt ccccacccgc atccgctaca cctcgatgag cctgccgtat cacatcggca
     2041 acggctggtt cggggggctg ctgcccacca cctcgttcgc catcgtggcg cagaccggca
     2101 acatgtacaa cggcctgtgg tatccgatca tcgtcgccgg catgacgctg gtgatcggca
     2161 tgctgttctt ccgcgaaacc aaggacgtgg acatctacgc cgaggattga gcggcggggc
     2221 gcgccccggc cgtcgcggcc ggggcgcgca tgccgtgacc gttcccccat gctcacccca
     2281 gtgccaggcc ttgcccggcg gctggggtga gctggttttc agcccccaac gacctgtttc
     2341 aaggagtcga acatgtggaa aattgccctc ggtttcgtgg tgttcgccgc gatttccctc
     2401 tacgtcatca tgcaggccgg cgacaaggtc gacatgagcg gtgaaaagca cggcgccgac
     2461 gccgtgcacg tgcccgacgc cccggcctcc gccgccaagt gaagctcggg ccatgcgcgc
     2521 agatgaccga tccggcggcc cgcgggccgc accgacctga aacggcgccc aggcgccgtt
     2581 tttcatgccc ggtcgggcca cgccgggcag ccggcggagc gcttcagccg gcaaacaccg
     2641 gtggcgcgca ggccgattcg cgccgcccgc gccctgcgcc cggcacgctc ctagaatctt
     2701 cgggcccctt cacgacggac ccgcaatgac cgatggcctg atccgcatcc gcggcgcgcg
     2761 gcagcacaac ctgaagaacc tggacctgga cctgcgcacc ggcgagctga ccgtggtcac
     2821 cggcgtcagc ggctcgggca agtccagcct ggtgttcgac accctgtacg ccgagggcca
     2881 gcggcgttac gtcgagacct tcagccccta cgcgcgccaa ttcctcgacc gcatggacaa
     2941 gccggcggtg gaccaggtcg aaggcgtgcc gccggccatc gccatcgacc agactaatcc
     3001 ggtgcgcaac tcgcgctcca ccgtgggcac gatgacggag ctgaacgacc acctcaagct
     3061 cctgttcgcg cgcgccggcc ggctgttcga ccgccagacc gcgctggcgg tgcggcatga
     3121 cacgccggac agcatctttg ccgagctgca ccggcgcgcc ggcgagcatg gcgacctacg
     3181 cctggtggcg accttcgcgg tggagctgcc ggccagcaca accgccgagg acgtcgagca
     3241 gtggctgtca gcggccggct tcacgcgcgt gcaggccgag cgcacggaag gcgacaagaa
     3301 gatcctggat gtcgtcgccg accgttttcg catgggcggc gccgagcgcg cgcgcgtgat
     3361 cgaggccatc gagctggccc tgctgcgcgg cggcgggcgc atgacggtgt acgccgtgcc
     3421 ggaagcaggc gaccccgagc cgtggaaatt ctccaccggc ctgcattgcc cggagagcga
     3481 catccgctac ggcgatccga ttccgtcgat gttctcgttc aactcggcgg tcggcgcctg
     3541 ccccgactgc cggggcttcg gccgcgtgat cggcgtcgac ttcggcctgg tgatcccgga
     3601 tggccgcctg accctgcgcg ccggcgccat caagccattc cagaccccgg cctggagcga
     3661 atgccaggac gacctgatgc gccacgccga aggcgccggc atcccgcgcg acacgccctg
     3721 gaacaagctg acgcccgagc agcagcgctg ggtgatcgag ggcacgccca gctaccgcga
     3781 agggcagtgg agcaagcagt ggtacggcgt gcggcgcttc ttcgcctatc tggagagcaa
     3841 ggcgtacaag atgcacatcc gcgtgctctt gtccaaatac cgcagctaca cgccctgtcc
     3901 cagctgcggc ggcgcgcggc tgaaactgga gtcgctgctg tggcgcgtgg gcagcaagga
     3961 agatgcggat gccgtgctgg agccgcgcca aagattcatg ccgcaaggcg tggcgtggac
     4021 gcgcgaacag ctcgaaaccc tgcccggcct gtgcctgcac gacctgatgc tgctgcccat
     4081 cgatcggctg gcgagcttct tcaaccggct tcatggcgct tcaaaaaatg gagctgctgg
     4141 cgcgcatcag gaaagcgccg aaggccattt tgatgctcca tccgccgacg cccaggcgct
     4201 caagctgatc ctcgatgaaa tccagacccg cctgaagtac ctgaccgacg tcggcatcgg
     4261 ctacctcacg ctcgaccgcc agagccgcac gctgtcgggt ggcgaggtgc agcgcatcaa
     4321 cctgaccacg gcgctgggca cctcgctggt gaacacgctg ttcgtgctgg acgagcccag
     4381 catcggcctg cacccgcgcg acatggaccg catcacccag gccatgcagc gcctgcgcag
     4441 cgccggcaac accctggtgg tggtcgagca cgacccggcg gtgatgctgg cggccgaccg
     4501 cgtgctcgac ttcggccccg gcccgggcgc ggccggcggg cgcatcgtgt tcgacggcac
     4561 gccgggtgaa ctgcgcggcg ccgacacctt gaccggcgcg tacctgggcg ggcgcaagtc
     4621 gatcggcctg ggcttctcgc gtttggtgaa ggccgacacc ccgcgcctgc tgctggaagg
     4681 cgtgcgcgag cacaacctgc gcggcattga cgtcgagttc ccgctgcagc gcctggtcac
     4741 cgtgacgggg gtgtcgggct cgggcaagtc caggctgatc caggacgtgc tggcgccggc
     4801 gctgatgcgc cacttcggcc agtcgaccga atcaccgggc gcgcacgacc gcctgctggg
     4861 cgccgagcag ctcagcggcg tggtgttcgt cgaccagtcg cccatcggca agacggcgcg
     4921 ctccaacccg gtgagctacg tcggcgcctg ggacgccatg cgcaagctgt ttgccgacgc
     4981 gccgctggcg cgccagcgtg gctacaccgc gtccaagttc agcttcaac
//
LOCUS       DAOOOG010000178         5026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_20068_length_5026_cov_27.802454, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000178 DAOOOG010000000
VERSION     DAOOOG010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20068_length_5026_cov_27.802454"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PK242_11275"
     CDS             <1..278
                     /locus_tag="PK242_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011205166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOZ94644.1"
                     /translation="CDSLLIGPHCGAHTFPYVDSGRADAVVEHEATTTRISAERLFYC
                     EQRGIDAQEATALIVGGFCQAVLEELPMEFALEAKALLAVSLEGAVG"
     gene            360..1130
                     /gene="sufC"
                     /locus_tag="PK242_11280"
     CDS             360..1130
                     /gene="sufC"
                     /locus_tag="PK242_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOZ94645.1"
                     /translation="MTSPEPLLQVRDLRVQVGERTVLHSVSLDVNAGSLVVLMGANGS
                     GKSTLGLALAGHPAYQVLGGQALLAGQDLLALPAHERARAGLFLSFQSPPDVPGVKNN
                     LFIRTALNAQRESRGQPPLDAFDFLTSAKQAAKALGLPEAMLGRPVNEGFSGGERKRN
                     ELLQLALLQPRLALLDEIDSGMDVDGVRAVVEQVRRQRAQGTAFIVVSHYLQMIEQLE
                     PDAVLRLDQGCIAETGDLGLARHIAETGFASRTAPAGA"
     gene            1132..2370
                     /locus_tag="PK242_11285"
     CDS             1132..2370
                     /locus_tag="PK242_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOZ94646.1"
                     /translation="MNPSQAERLAARQHLQRQGWIARRSEAFHHLPPPELAHWLGQPE
                     ADADADDGNAPALAGAGWTLHPLGHGGAHGVDARWLDALDPAQRAELLAGLPPPGDGE
                     AAPFVWAHRALCRQGLRLRLSATPGQPAAPVVLQLRHQPHQRAEAPLLVLEVDAGVHC
                     VLLETHERTAADGAHPVTQNLHGHIRLAAGAHLQHLRVVAPQAQDQIAHHLHVSLAPA
                     AHYAQALVATGSAYHLQRSTVQLSGRGARAQQAALLLLDDQQLDHQAHSDLAAAHSAS
                     AVESLVLAQGGARAVSNAYTRIAAGADEAEVRQRLSGIPLGGQPRLILRPHLEILHDN
                     VQAAHGATWGALPADALFYARQRGLDEASARALITEGMARAVLERSLGDTELLSDWLA
                     TGWLTQRMARQARQRAETPA"
     gene            2428..3717
                     /locus_tag="PK242_11290"
     CDS             2428..3717
                     /locus_tag="PK242_11290"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HOZ94647.1"
                     /translation="MPNHQPPLTLPEADRAAAAPPPLAAWRAQFPVLSQQIHGHALAY
                     LDNAATTHMPLAVMEAMRRFETHERANIHRGVHTLSQRATDAFEQARATLKRFIGADA
                     GHELVFTSGTTESLNLVAQGLWGAGPGQAWLRPGDRIISTGLEHHANLVPWQHAARMS
                     GAELVVLRPDAQGRLQQDALAALLTPRTRVLAITASANATGERPPYEAMLAMARQAGA
                     LTVLDAAQAVGHGMPALSALACDFMAFSGHKMYGPMGIGVLAGRRDALERLHPLRLGG
                     DMVEDVSYERAIYAALPARLEGGTPNVAGAVGLAAAATFIDRIGRAAIDGHLHALRAQ
                     AVAGLSALPGVTVLAPHATQAALVSFVADQVHPHDIGTLLDERGIAVRTGHHCAQPLH
                     DHFGLGPTTRASFALYNSAEEVERLIAAVARALEMLR"
     gene            3714..4175
                     /locus_tag="PK242_11295"
     CDS             3714..4175
                     /locus_tag="PK242_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOZ94648.1"
                     /translation="MSSAENDLYQEVVLEHKRAPRNFGHLPQPTHQAQGTNPSCGDRV
                     AVELLLDGERVQDIRFSGQGCAICMASSSMMTEAVKGRDIAAAQALQQHFRAVLTGEE
                     DPDEAPLGKLVSLAGVRQYPSRIKCALLGWHALMHAIAGQQDAGGTEESLP"
     gene            4172..4726
                     /gene="sufT"
                     /locus_tag="PK242_11300"
     CDS             4172..4726
                     /gene="sufT"
                     /locus_tag="PK242_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HOZ94649.1"
                     /translation="MMNRRHREDVLVRRHVTVETIPSGHPVELEPGQLAQITQALGGS
                     FTLLVEGQLMRLKGQDADAIGKQPPEALAVPKDLPIEELEPLVWDTLRTCYDPEIPVN
                     IADLGLVYRLAFEPLDDPDQVRVVIDMTLTAPGCGMGESIANEVCDKVLALPRVGEIT
                     VNLVFDPPWDRSMMSEAAQLALGL"
     gene            complement(4793..>5026)
                     /locus_tag="PK242_11305"
     CDS             complement(4793..>5026)
                     /locus_tag="PK242_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HOZ94650.1"
                     /translation="HQQIFAQVDALQVGQALQIVNDHDPVPLRRQLEARSPGQLQWAY
                     VQAGPDLWRVQISKVQGAADAEGSCCSGGACCG"
BASE COUNT          747 a         1944 c         1717 g          618 t
ORIGIN      
        1 agtgcgattc gctgctgatc ggcccgcact gcggcgccca caccttcccc tacgtcgaca
       61 gcgggcgcgc cgacgccgtg gtggaacacg aggccaccac cacccgcatc tcggccgagc
      121 ggctgttcta ctgcgaacag cgcggcatcg acgcccagga ggccacggcg ctgatcgtcg
      181 gcggcttctg ccaggccgtg ctggaagagc tgccgatgga gttcgcgctc gaagccaagg
      241 cgctgctggc agtgtcgctg gaaggcgcgg tgggctgaac gaatagccat caaacaggcc
      301 gcccgcgctt acccagcaag cgccagcagc tatcaaaata ttagcaaaag gattccctga
      361 tgacctcccc cgaacccctg ctgcaggtgc gcgatctgcg cgtccaggtg ggcgagcgca
      421 ccgtgctgca ctcggtctcg ctcgacgtca acgccggcag cctggtcgtg ctgatgggcg
      481 ccaacggcag cggcaaaagc acgctgggcc tggcgctggc cggccacccg gcctaccagg
      541 tgctgggcgg ccaggcgctg ctggccgggc aggacctgct ggcgctgccg gcgcacgagc
      601 gcgcgcgcgc cggcctgttc ctgtcgttcc agtcgccgcc cgacgtgccg ggcgtcaaga
      661 acaacctgtt catccgtacc gcgctcaacg cgcagcgcga atcgcgcggc cagccgccgc
      721 tcgacgcctt cgacttcctc accagcgcca agcaggccgc caaggccctg gggctgcccg
      781 aagccatgct gggccgcccg gtcaacgagg gcttttccgg cggcgagcgc aagcgcaacg
      841 agctgctgca gctggcgctg ctgcagccgc gcctggcgct gctggacgaa atcgactccg
      901 gcatggacgt cgacggcgtg cgcgccgtgg tcgagcaggt gcggcgccag cgcgcgcagg
      961 gcacggcctt catcgtcgtg tcgcactacc tgcagatgat cgagcagctc gaacccgacg
     1021 ccgtgctgcg gctcgatcag ggctgcatcg ccgaaaccgg cgacctgggc ctggcacgcc
     1081 acatcgccga aaccggcttt gcctcgcgca cggcgccggc gggggcctga catgaacccg
     1141 tcgcaagccg aacgcctggc cgcgcgccaa cacctgcagc gccagggctg gatcgcgcgc
     1201 cgcagcgaag ccttccacca cctgccgccg cccgagctgg cgcactggct gggccagccg
     1261 gaggccgacg ccgacgccga cgacggcaac gccccggcgc tggccggcgc cggctggacg
     1321 ctgcacccgc tgggccacgg cggcgcccac ggcgtggacg cgcgctggct cgacgccctc
     1381 gacccggccc agcgcgccga gctgctggcc ggcctgccgc cgcccggcga cggcgaggcc
     1441 gcgcccttcg tctgggcgca ccgggcgctg tgccgtcagg gcctgcgcct gcgcctcagc
     1501 gccacgcccg gccagcccgc cgccccggtg gtgctgcagt tgcgccacca gccgcaccag
     1561 cgcgccgaag cgccgctgct ggtgctcgag gtcgacgccg gcgtgcactg cgtgctgctg
     1621 gaaacccacg aacgcaccgc cgccgacggc gcccatcccg tgacccagaa cctgcacggc
     1681 cacatccgcc tggcggccgg cgcgcacctg cagcacctgc gcgtggtggc gccccaggcg
     1741 caggaccaga ttgcccacca cctgcacgtc agcctggcgc cggcggcgca ctacgcgcag
     1801 gcgctggtgg ccaccggcag cgcctaccac ctgcagcgca gcaccgtcca actgagcgga
     1861 cgcggcgcgc gcgcccagca ggccgccctg ctgctgctgg acgaccagca gctcgaccac
     1921 caggcgcaca gcgacctggc ggcggcgcac agcgccagcg ccgtcgaatc gctggtgctg
     1981 gcccagggcg gcgcgcgcgc cgtcagcaac gcctacaccc gcatcgccgc cggcgccgac
     2041 gaggccgagg tgcgccagcg cctgtccggc ataccgctcg gcggtcagcc gcgcctgatc
     2101 ctgcgcccgc acctggaaat cctgcacgac aacgtgcagg ccgcgcacgg cgccacctgg
     2161 ggcgcgctgc ccgccgacgc gctgttctac gcccgccagc gcggcctcga cgaagccagc
     2221 gcgcgcgccc tgatcaccga aggcatggcg cgcgccgtgc tggagcgcag cctcggcgac
     2281 accgaactgc tgagcgactg gctggccacc ggctggctga cgcagcgcat ggcgcggcag
     2341 gcccggcagc gcgccgaaac ccccgcctga agactatgtt tttgatagct gcttgcgcaa
     2401 tccacatcag cgccagagcc ctttttgatg ccaaaccacc agccccccct caccctgcct
     2461 gaggccgacc gcgcggcggc cgcgccaccg ccactggcgg cctggcgggc ccagtttccg
     2521 gtgctgagcc agcagatcca cggccacgcg ctggcctacc tggacaacgc cgccaccacc
     2581 cacatgccgc tggcggtgat ggaggcgatg cgccgcttcg agacgcacga gcgcgccaac
     2641 atccaccgcg gcgtgcacac gctcagccag cgcgccaccg acgccttcga acaggcgcgc
     2701 gccacgctca agcgcttcat cggcgccgac gccgggcacg agctggtgtt caccagcggc
     2761 accaccgagt cactcaacct ggtggcccag ggcctgtggg gcgccggccc cggccaggcc
     2821 tggctgcgcc cgggcgaccg catcatcagc accggactgg aacaccacgc caacctggtg
     2881 ccgtggcagc acgccgcccg catgagcggc gccgagctgg tggtgctgcg ccccgacgcc
     2941 cagggccgcc tgcagcagga cgccctggcc gcgctgctga cgccgcgcac gcgcgtgctg
     3001 gccatcaccg ccagcgccaa cgccaccggc gagcgcccgc cctacgaagc catgctggcc
     3061 atggcgcgcc aggccggcgc gctgaccgtg ctggacgccg cccaggcggt gggccacggc
     3121 atgccggcgc tgagcgcgct ggcgtgcgac ttcatggcct tttccggcca caagatgtac
     3181 ggccccatgg gcatcggcgt gctggccggc cggcgcgacg cgctggagcg gctccacccg
     3241 ctgcgcctgg gcggcgacat ggtggaggac gtgagctacg agcgcgccat ctacgccgcg
     3301 ctgccggcgc gcctggaagg cggcaccccc aacgtggccg gcgcggtcgg cctggcggcg
     3361 gcggccacgt tcatcgaccg gatcgggcgc gccgccatcg acggccacct gcacgcgctg
     3421 cgcgcgcagg cggtggccgg cctgtcggcc ctgcccggcg tcaccgtgct ggcgccgcac
     3481 gccacgcagg cggcgctggt gtccttcgtg gccgaccagg tgcacccgca cgacatcggc
     3541 accttgctgg acgagcgcgg catcgccgtg cgcaccggcc accactgcgc ccagccgctg
     3601 cacgaccact tcggactggg gccgaccacg cgcgcctcgt tcgccctcta caacagcgcc
     3661 gaggaagtcg aacgcctgat cgccgccgtg gcgcgcgccc tggagatgtt gcgatgagca
     3721 gcgccgagaa cgatctctat caggaagtgg tgctggagca caagcgcgcg ccgcgcaact
     3781 tcggccacct gccgcagccc acccaccagg cccagggcac caacccgtcc tgcggcgacc
     3841 gcgtggcggt cgagctgctg ctggacggcg agcgggtgca ggacatccgc ttttccggcc
     3901 agggctgcgc catctgcatg gcctccagct cgatgatgac cgaggccgtc aagggccgcg
     3961 acatcgccgc cgcgcaggcg ctgcagcagc actttcgcgc cgtgctgacg ggcgaggaag
     4021 accccgacga agccccgctg ggcaagctgg tcagcctggc cggcgtgcgc cagtacccca
     4081 gccgcatcaa gtgcgcgctg ctgggctggc acgcgctgat gcacgccatt gccggccagc
     4141 aggacgccgg cggcaccgag gagtcgctgc catgatgaac cgccgccacc gcgaggacgt
     4201 gctggtgcgc cgccacgtca cggtggaaac cattcccagc ggccacccgg tcgagctgga
     4261 gcctggccag ctggcgcaga tcacgcaggc gctggggggc tccttcaccc tgctggtcga
     4321 gggtcagctg atgcgcctga agggccagga cgccgacgcc atcggcaagc agccgcccga
     4381 agccctggcc gtgcccaagg acctgccgat cgaggagctg gagccgctgg tgtgggacac
     4441 gctgcgcacc tgctacgacc cggaaatccc ggtcaacatc gccgacctgg gcctggtcta
     4501 tcggctggcg ttcgagccgc tggacgatcc cgaccaggtg cgcgtggtga tcgacatgac
     4561 cctcaccgcc cccggctgcg gcatgggcga atcgatcgcc aacgaggtgt gcgacaaggt
     4621 gctggcgctg ccgcgcgtgg gcgaaatcac cgtcaatctg gtgttcgacc cgccctggga
     4681 ccgttccatg atgtccgaag cggcgcagct ggccctgggg ctataggccc ccggcaacaa
     4741 aaaagcgcgc cagcccttgc gccggcgcgc cggtgcgtct tgcccgaccc ggtcagccgc
     4801 aacaggcgcc gcccgagcag cacgagcctt cggcgtcggc cgcgccctgc accttgctga
     4861 tctgcacgcg ccacaggtcc gggccggcct gcacataggc ccactgcagc tggccgggcg
     4921 agcgcgcttc cagctggcgg cgcagcggca ccgggtcgtg gtcgttgacg atctgcagcg
     4981 cctggcccac ctgcagcgcg tccacctggg caaagatctg ctggtg
//
LOCUS       DAOOOG010000179         5009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_20162_length_5009_cov_33.693783, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000179 DAOOOG010000000
VERSION     DAOOOG010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20162_length_5009_cov_33.693783"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PK242_11310"
     CDS             <1..398
                     /locus_tag="PK242_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOZ94651.1"
                     /translation="FAVERYSHVMHIVSNVEGLLKDGLSAMDVLKATFPAGTLSGAPK
                     VHAMELIDQLEPVKRGIYGGACGYWSYAGDMDLAIAIRTAIVKDQMLHVQAAAGVVAD
                     SVPEMEWRETEHKARALLRASELVEEGLE"
     gene            416..994
                     /locus_tag="PK242_11315"
     CDS             416..994
                     /locus_tag="PK242_11315"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOZ94652.1"
                     /translation="MQLLMIDNYDSFTFNIVQYLGELGAEVTVVRNDEITVDDIQRRL
                     DAGQVDRLVISPGPCSPAEAGISVPAIQRLAGQVPILGVCLGHQAIGAAFGAPIIRAQ
                     TQMHGKTSVISTDQQGVFAGLPRQFTVNRYHSLVIDRHAVPECLHVTATSEDGEIQGV
                     RHRTLAIEGVQFHPESILTEHGHAMLANFLRS"
     gene            1009..1689
                     /locus_tag="PK242_11320"
     CDS             1009..1689
                     /locus_tag="PK242_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOZ94653.1"
                     /translation="MPDTAQLLLFITAGLVLNLTPGPDVMYIVAHAVRGGARAGVAAA
                     LGIAAGCLVHVAAAALGVSALLATSATAFALLKWAGAFYLVWVGVQMLRAALRPDATD
                     NIAAGAIPASAGAGLHAQVPRAAVWRRGFLTNVLNPKVALFFLAFVPQFIAPGTAQPG
                     LVFLLLGLLFTVNGLLVCVGWALAAAWAARRAGALQRALRWLDGVAGGLFIVFGVRLA
                     LTQAPGAR"
     gene            1732..2766
                     /gene="trpD"
                     /locus_tag="PK242_11325"
     CDS             1732..2766
                     /gene="trpD"
                     /locus_tag="PK242_11325"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOZ94654.1"
                     /translation="MAITPQEALQRTIEHREIFHDEMLHLMRLIMRGEISPLMVAAIT
                     TGLRVKKETIGEITAAAQVMRELSHKVNVTDRSHLVDVVGTGGDGAHTFNISTCAMFV
                     AAAAGARVAKHGGRSVSSKSGSADVMEALGVPLTLTPEQIARCIADSGVGFMFAPNHH
                     PAMKNVAPVRKEMGVKTFFNILGPLTNPAGAPNILMGVFHPDLVGIQVRALQRLGAEH
                     AVVVYGRDGMDEVSLGAATLVGELKDGEIREYEIHPEDFGLRMQSNRSLRVETPEQSV
                     AMLRGVLDNQEGAARDIVALNAGVVLYAAGVAPSMMAGIERASQAIESGAARAKLAQV
                     VALTQSLAAA"
     gene            2778..2921
                     /locus_tag="PK242_11330"
     CDS             2778..2921
                     /locus_tag="PK242_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94655.1"
                     /translation="MWQDEGTWIALGISALFLVAALVMHRVFVKILKNPAPDERKELS
                     KDE"
     gene            2914..3717
                     /gene="trpC"
                     /locus_tag="PK242_11335"
     CDS             2914..3717
                     /gene="trpC"
                     /locus_tag="PK242_11335"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOZ94656.1"
                     /translation="MSDILDRIVDVKREEVAAGLKKKSLAAMRADAESRVLTRDFVGA
                     LRAKVAAGQAAVIAEIKRASPSKGVIRPGEFIPADLAQSYAQGDGQISAACLSVLTDR
                     QFFQGGIDYLKQARASCGLPVLRKDFMIDPWQVYEGRAIGADAILLIAACLSDGQMAE
                     LEAVALGLDMAVLVEVHDGAELQRALRLKTPLVGINNRNLRSFDTTLETTLGLLADVP
                     ADRLLVTESGIASRADVQRLRAAGVQAFLVGETFMRADEPGLALAELFR"
     gene            3775..4542
                     /locus_tag="PK242_11340"
     CDS             3775..4542
                     /locus_tag="PK242_11340"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOZ94657.1"
                     /translation="MLNRAGQGAALGQWPCDLDEVAAGWRPLLQAFLASAEGRRLSEG
                     LRARLAAGATIYPPQPLRALQLTPPEAVRAVILGQDPYHGPGQACGLAFAVAPGVRPP
                     PSLRNIFKELAREFGRPPQTEQHALAHWARQGVLLLNTSLTVEAGQAASHARIGWQVL
                     TSQIFHRLVHGPRPLAFLLWGAHAQALRPQGAEAGRHLWLVANHPSPLSALRPPVPFI
                     GCGHFGRVNDFLRQQGEPEIDWLGTAAPASPLSGPAV"
     gene            4567..4652
                     /locus_tag="PK242_11345"
     tRNA            4567..4652
                     /locus_tag="PK242_11345"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4601..4603,aa:Tyr,seq:gta)
     gene            4707..4780
                     /locus_tag="PK242_11350"
     tRNA            4707..4780
                     /locus_tag="PK242_11350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4739..4741,aa:Gly,seq:tcc)
     gene            4812..4886
                     /locus_tag="PK242_11355"
     tRNA            4812..4886
                     /locus_tag="PK242_11355"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4844..4846,aa:Thr,seq:ggt)
BASE COUNT          799 a         1672 c         1757 g          781 t
ORIGIN      
        1 cctttgccgt ggagcgctac agccacgtga tgcacatcgt cagcaacgtc gagggtctgc
       61 tcaaggacgg cctgagcgcc atggacgtgc tcaaggccac cttcccggcc ggcaccctga
      121 gcggcgcacc caaggtgcac gccatggagc tgatcgacca gttggagccg gtcaagcgcg
      181 gcatctacgg cggcgcctgc ggctactgga gctacgccgg cgacatggac ctggccattg
      241 cgattcgcac cgccatcgtc aaggaccaga tgctgcacgt gcaggcggcg gccggcgtgg
      301 tggccgacag cgtgcccgag atggaatggc gcgagaccga gcacaaggcg cgcgccctgc
      361 tgcgggcgag cgagctggtg gaagaaggac tggagtgagc gccatggcga aaaagatcca
      421 gctgctcatg atcgacaact acgacagctt caccttcaac atcgtgcagt acctgggcga
      481 gctcggcgcc gaggtgaccg tggtgcgcaa cgacgaaatc acggtcgacg acatccagcg
      541 ccgcctggat gccggccagg tggaccgcct ggtcatctcg cccggcccct gctcgccggc
      601 cgaggcgggc atctcggtgc cggcgatcca gcgcctggcc ggccaagtgc ccatcctggg
      661 cgtgtgcctg ggccaccagg ccatcggcgc ggcgttcggc gcgcccatca tccgcgcgca
      721 gacgcagatg cacggcaaga ccagcgtcat cagcaccgac cagcaaggcg tgtttgccgg
      781 cctgccacgg cagttcacgg tcaaccgcta ccactcgctg gtgatcgacc gccacgccgt
      841 gcccgagtgc ctgcacgtga cggccaccag cgaggacggc gaaatccagg gcgtgcgcca
      901 ccgcacactg gcgatcgagg gcgtgcagtt tcaccccgaa agcatcctga ccgagcatgg
      961 ccacgccatg ctggccaact ttcttcgctc ctgaaggcca gacccgccat gcccgacacc
     1021 gcacaactgc tgctgttcat taccgccggt ctggtgctca acctgacgcc ggggccggac
     1081 gtgatgtaca tcgtggccca cgcggtgcgc ggcggcgcgc gggccggcgt ggcggctgcc
     1141 ctgggcatcg ccgccggctg cctggtgcat gtggcggcgg cggcgctggg cgtgtcggcg
     1201 ctgctggcca ccagcgccac ggcgttcgcg ctgctcaagt gggccggtgc gttctacctg
     1261 gtgtgggtgg gggtgcagat gctgcgcgct gcgctgcgcc cggatgctac tgataacata
     1321 gctgctggcg caataccagc aagcgctgga gccggattgc atgctcaagt gccgcgcgcc
     1381 gcggtgtggc gtcgcggctt tctgaccaac gtgctcaacc ccaaggtggc gctgtttttt
     1441 ctggccttcg tgccgcaatt catcgcgccc ggcacggcgc agccgggcct ggtgtttctg
     1501 ctgctggggc tgctgttcac cgtcaatggc ctgctggtgt gcgtcggctg ggcgctggcg
     1561 gcggcttggg cggcgcgtcg ggccggcgcc ctgcagcgcg cgttgcgctg gctggacggc
     1621 gtggcgggcg ggctgttcat cgtgttcggc gtgcggctgg cgctgacgca ggcgcccggc
     1681 gcgcgctgac ggacccattt cttccaacga gtttttcaag gcgaagtcac catggccatc
     1741 accccgcagg aagcgctgca gcgcaccatc gagcaccgcg agatcttcca tgacgagatg
     1801 ctgcacctga tgcggctgat catgcgcggc gagatctcgc cgctgatggt ggcggccatc
     1861 accaccggcc tgcgcgtcaa gaaggaaacc atcggcgaaa tcaccgccgc cgcgcaggtg
     1921 atgcgcgagc tgtcgcacaa ggtgaacgtg accgaccgca gccacctggt ggacgtggtg
     1981 ggcaccggcg gcgacggcgc gcacaccttc aacatcagca cctgcgccat gttcgtggcc
     2041 gcagccgccg gcgcgcgcgt ggccaagcat ggcgggcgca gcgtgtccag caagtcgggc
     2101 agcgccgacg tgatggaggc actgggcgtg ccgctgacgc tgacgcccga acagatcgcg
     2161 cgctgcatcg ccgacagcgg ggtgggtttc atgttcgccc ccaaccacca tccggcgatg
     2221 aagaacgtgg cgccggtgcg caaggaaatg ggcgtcaaga ccttcttcaa catcctgggg
     2281 ccgctcacca acccggccgg cgcgcccaac atcctgatgg gggtgtttca ccccgatctg
     2341 gtgggcatcc aggtgcgcgc gctgcagcgc ctgggcgccg agcacgcggt ggtggtgtac
     2401 ggccgcgacg gcatggacga agtcagcctg ggcgccgcca ccctggtggg cgagctcaag
     2461 gacggcgaaa tccgcgagta cgagatccac cccgaggact tcggcctgcg catgcagagc
     2521 aaccgcagtc tgcgggtcga gacgccggag caatcggtcg ccatgctgcg cggcgtgctg
     2581 gacaaccagg agggcgcggc gcgcgacatc gtggcgctca acgccggcgt ggtgctgtac
     2641 gcggcgggcg tggcgcccag catgatggcc ggcatcgagc gcgcgtccca ggccatcgaa
     2701 agcggcgccg ccagggccaa gctggcgcag gtggtggcgc tgacgcagtc gctggcggcg
     2761 gcctgatcgg tggcgccatg tggcaggacg aaggcacctg gatcgccctg ggcatctcgg
     2821 ccctgtttct ggtggcggcg ctggtgatgc accgcgtgtt cgtcaagatt ctgaaaaacc
     2881 cggcgcccga tgagcgcaag gagctatcaa aagatgagtg acattctgga ccggatcgtg
     2941 gacgtcaagc gcgaggaagt ggccgccggc ctgaagaaga agtcgctggc ggcgatgcgc
     3001 gccgacgccg aaagccgtgt gctgacgcgc gacttcgtgg gcgcgctgcg cgccaaggtg
     3061 gcggccggcc aggccgccgt gatcgccgag atcaagcgcg ccagccccag caagggcgtg
     3121 atccggcccg gcgagttcat ccccgccgac ctggcgcaga gctacgccca gggcgacggc
     3181 cagatcagcg ccgcctgtct gtcggtgctg accgatcgcc agttcttcca gggcggcatc
     3241 gactacctga agcaggcgcg cgccagctgc ggcctgccgg tgctgcgcaa ggacttcatg
     3301 atcgatccct ggcaggtgta cgaggggcgc gccatcggcg ccgacgccat cctgctgatc
     3361 gccgcctgcc tgtccgacgg ccagatggcc gagctggaag cggtggcgct gggcctggac
     3421 atggcggtgc tggtcgaggt gcacgacggc gccgaactgc aacgcgcgct gcggctgaaa
     3481 accccgctgg tcggcatcaa caaccgcaac ctgcgcagct tcgacaccac gctggagacc
     3541 acgctggggc tgctggccga cgtgccggcc gaccgcctgc tggtcaccga aagcggcatc
     3601 gccagccgcg ccgacgtgca gcgtttgcgc gccgccggcg tgcaggcctt cctggtcggc
     3661 gaaaccttca tgcgcgccga cgagccgggg ctggcgctgg ccgaactgtt tcgctgaacg
     3721 ctatcattaa gatagctgac ggcgcaatct gggtaagcgg tggactccca tggaatgctg
     3781 aatcgggccg ggcagggcgc cgcgctgggc cagtggccct gcgacctgga cgaggtggcg
     3841 gccggctggc gcccgctgct gcaggcgttt ctggccagcg ccgaaggccg gcggctgagc
     3901 gaagggctgc gggcgcggct ggcggccggt gccaccatct acccgccaca gccgctgcgc
     3961 gcgctgcaac tgaccccgcc cgaggcggtg cgcgccgtca tcctcggtca ggacccgtac
     4021 cacgggcccg ggcaggcctg cggtctggcg tttgccgtcg cccccggggt gcgcccgccg
     4081 cccagcctgc gcaacatctt caaggagctg gcgcgcgaat tcggtcggcc gccccagacc
     4141 gagcagcacg ccctggcgca ctgggcgcgc cagggcgtgc tgctgctcaa caccagcctc
     4201 accgtcgaag cgggccaggc cgccagccac gcccgcatcg gctggcaggt gctgacttcg
     4261 cagatctttc accggctggt gcacggccca cggccgttgg cgttcctgct gtggggtgcg
     4321 catgcccagg cactgcgacc tcagggagcc gaggccgggc gccacctctg gctggttgcc
     4381 aaccatccgt caccgctgtc ggcgctgcgc ccgcccgtgc ccttcatcgg ctgcgggcat
     4441 ttcgggcgcg tcaacgactt cctgcgccag cagggcgagc cggagatcga ctggctgggg
     4501 acggccgcgc ccgccagccc cttgtctggc ccggcggttt gatggcataa tcgcaagttc
     4561 tctgccggag aggtggccga gtggttaatg gcagcagact gtaaatctgc cctcttacga
     4621 gtacgctggt tcgaatccag ccctctccac cagcagacaa aggtgcgcaa ggcaggtcac
     4681 ggcgcgaggc gtggcggcca ggaactgcgg gagtagttca atggtagaac cttagccttc
     4741 caagctaatg acgcgggttc gattcccgtc tcccgctcca gaggctggcc ggtgcgtcga
     4801 aaagaaacat tgcccttgtg gctcagtggt agagcactcc cttggtaagg gagaggtcgc
     4861 gggtccgatt cccgccaagg gcaccaggat tgcggtgatg ccagggtgca tcaccgcttt
     4921 cggtgtgttg tttgagttga tttttcggag tcttcgacat ggcaaaagag aaatttgagc
     4981 gcagcaagcc gcacgtgaac gtcggcacc
//
LOCUS       DAOOOG010000180         4989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_20286_length_4989_cov_28.912444, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000180 DAOOOG010000000
VERSION     DAOOOG010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20286_length_4989_cov_28.912444"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PK242_11360"
     CDS             complement(<1..1015)
                     /locus_tag="PK242_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ94658.1"
                     /translation="MARPLFVRGAVVLAALALTGAAQAAALRIAGANDILTFDPHAQN
                     HQTTLAYQQMVYESLVRYTEKYEVEPSLATKWTFVTPTQLRFELRKGVKFHDGAPLTA
                     DDVVFSLNRAMTPPSNMTAYVQSVKEVKKVDDHTVDVILKAPSPVVLRELTDARIMNK
                     AWAEKNNAVKAQDYKAKEENFAARNANGTGPFILEAWQPDTKTTLKKNDAWWDKPKGN
                     VDSIVFTPIKAAATRSAALISGQVDMVVDPPVQDLERIKRDPNISVIEGAENRTIFIG
                     FDQFRDELLGSNVKGKNPLKDKRVRQALYQAVDIAAIERNVMRGLGKPTGTMIAPMVN
                     GWTK"
     gene            complement(1250..2938)
                     /gene="yidC"
                     /locus_tag="PK242_11365"
     CDS             complement(1250..2938)
                     /gene="yidC"
                     /locus_tag="PK242_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOZ94659.1"
                     /translation="MKDIRRTILWVIFAFSLVMLWDQWQVHNGRKATFFPGAPTPASQ
                     ASAPASAVPAASAQPAAVPGVAPAGVPPGDAAPAAAAAAPRERIEVTTDVLKLVLDTE
                     GGSIVRSEFLKFDQTEHPGQAFVLLDDSPQRTYQAQSGLIGGPFPTHKTAMRFTGERT
                     LAEGASELVLRFESPELGGLKLVKTYTLRRGDYVAQVRHEVVNTGGQPVSPQLYVQLV
                     RDGNPAPGGTSFYSTFTGPAFYTDAKKFQKVEFADIDKGKAEFATPSPDGWVAMVQHY
                     FASAWLLPQGMGHDNFARKLDGGLYAAGMIASLGAIAPGASQAVQARLFIGPQDEKVL
                     EGIAPGLELVKDYGYFTILAKPLYWLLDKLHGVLGNWGWAIVALVVLLKIAFYWLNAK
                     AYASMAKMKAINPRIQELRERLKDNPQQMQMEMMRIYREEKVNPMGGCLPIMIQIPVF
                     IALYWVLLSSVEMRGAPWILWIKDLSVKDPYFILPVVMTLSTLLQTALNPLPPDPMQA
                     RMMWIMPLVFSVMFFFFPAGLVLYWVTNNILTIAQQWIINTRMGVPPQFNLPKF"
     gene            complement(2935..3222)
                     /gene="yidD"
                     /locus_tag="PK242_11370"
     CDS             complement(2935..3222)
                     /gene="yidD"
                     /locus_tag="PK242_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOZ94660.1"
                     /translation="MMRALLIGLVKAYRLLLSPWLGSSCRFEPTCSLYAIGALQRHGA
                     LAGSYLTLRRLLRCHPGCDGGCDPVPPQPPRFFTRLIDRERSDPSTPRQPS"
     gene            complement(3219..3662)
                     /locus_tag="PK242_11375"
     CDS             complement(3219..3662)
                     /locus_tag="PK242_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOZ94661.1"
                     /translation="MSGVDATAAPALRSATPGRLRTREQFQALLAQRPVARTAHFALH
                     RLAGPQGLPDAVAPGLFCPAGVAWLGAMAPKRWARRAVTRNLIRRQIYAVGERLQPPL
                     AAGAHLVRLRAAFAPAQYPSAASVALQRAVRAELCQLFAQGRSAS"
     gene            complement(3688..3822)
                     /gene="rpmH"
                     /locus_tag="PK242_11380"
     CDS             complement(3688..3822)
                     /gene="rpmH"
                     /locus_tag="PK242_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOZ94662.1"
                     /translation="MKRTYQPSKTRRARTHGFLVRMKTRGGRAVINARRAKGRKRLAV
                     "
     gene            4224..>4989
                     /locus_tag="PK242_11385"
     CDS             4224..>4989
                     /locus_tag="PK242_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HOZ94663.1"
                     /translation="MHIDQPQRASASHGAALWDTCLETLARELPEQQFNTWIKPLSAQ
                     LAADHSRLTLYVGNRFKLDWVRAQYATRIAAVLEALQGHAVQVELALAPRAAPIKPSF
                     SIPTSETTQGDPEPARAPEPLPAPTGDRDSAGPVRTRLNGALTFDTLVEGTANRMARA
                     AALHVAGAPGQLYNPLFIYGGVGLGKTHLMHAVGNRLLQTRPDAKVLYIHAEQFVSDV
                     VKAYQRKTFDQFKERYHSLDLLLIDDVQFFANKDRTQ"
BASE COUNT          792 a         1702 c         1703 g          792 t
ORIGIN      
        1 ccttggtcca gccgttgacc atgggcgcga tcatggtgcc ggtgggcttg cccagaccgc
       61 gcatcacgtt gcgctcgatg gcggcgatgt ccaccgcctg gtagagcgcc tggcgcaccc
      121 gcttgtcctt gagcgggttc ttgcccttga cgttggagcc cagcagctcg tcgcggaact
      181 ggtcgaagcc gatgaagatg gtgcggtttt cggcgccctc gatgacgctg atgttggggt
      241 cgcgcttgat gcgctcgagg tcctgcaccg gcgggtcgac caccatgtcg acctggcccg
      301 aaatcagcgc cgccgagcgc gtggccgccg ccttgatggg ggtgaacacg atgctgtcga
      361 cgttgccctt gggcttgtcc caccaggcgt cgttcttctt cagcgtggtc ttggtgtcgg
      421 gctgccaggc ttccaggatg aaggggccgg tgccgttggc gttgcgagcg gcgaagttct
      481 cttccttggc cttgtagtcc tgcgccttga cggcgttgtt cttctcggcc caggccttgt
      541 tcatgatgcg cgcgtcggtc agctcgcgca gcaccaccgg gctgggcgcc ttcaggatca
      601 cgtccaccgt gtggtcgtcg accttcttga cttccttgac gctctgcacg taggccgtca
      661 tgttggacgg cggcgtcatg gcgcggttga gcgagaacac cacgtcgtcg gcggtgaggg
      721 gcgcgccgtc atggaacttg acgcccttgc gcaattcgaa gcgcagctgg gtcggcgtga
      781 cgaaggtcca cttggtggcc agcgacggct cgacctcgta tttttcggtg tagcgcacca
      841 gcgactcata gaccatctgc tggtaggcca gggtggtctg gtggttctgc gcgtgcggat
      901 cgaaggtcag gatgtcgttg gcgccggcga tgcgcagcgc ggcggcctgg gcggcgcccg
      961 tcagggccag ggcagccaac accacggcgc cacgaacgaa caggggacga gccatgaaat
     1021 caccttccag tgaagttgga aagtgcccat gctaccgaag aattggcatc gaattcgggc
     1081 ttcccggggt ttccccgggg gtcggcattg tcgacgatta ctatgaattc aatagctgct
     1141 tgcgcacaac aggcaagcgc tggcgatcga attcttcaac gttccgatgc cagtcgggtg
     1201 accgacccac cgcggccggc gaggcgcgcc ggccgcggcg gcacagggct cagaacttcg
     1261 gcaggttgaa ctgcggcggc acgcccatgc gggtgttgat gatccactgc tgggcgatgg
     1321 tcaggatgtt gttggtcacc cagtacagca ccagaccggc ggggaagaag aagaacatca
     1381 cgctgaacac cagcggcatg atccacatca tcctggcctg catcgggtcg ggcggcaggg
     1441 ggttgagcgc ggtctgcagc agggtgctga gcgtcatcac caccggcagg atgaagtacg
     1501 ggtccttcac cgacaggtcc ttgatccaca ggatccaggg cgcgccgcgc atttccacgc
     1561 tggacagcag cacccagtac agggcgatga acaccgggat ctggatcatg atgggcagac
     1621 agccgcccat cgggttgacc ttttcctcgc ggtagatgcg catcatctcc atctgcatct
     1681 gctgcgggtt gtccttcagg cgctcgcgca gctcctggat gcgcgggttg atggccttca
     1741 tcttggccat gctggcgtag gccttggcgt tgagccagta gaaggcgatc ttcagcagca
     1801 ccaccagggc cacgatggcc cagccccagt tgcccagcac gccgtgcagc ttgtcgagca
     1861 gccagtacag cggcttggcc aggatggtga agtagccgta gtccttgacc agctccagcc
     1921 ccggggcgat gccttccagc accttctcgt cctgcgggcc gatgaacagg cgcgcctgca
     1981 ccgcctggct ggcgcccggg gcgatggcgc ccagcgaagc gatcatgccg gcggcgtaca
     2041 gaccaccatc gagcttgcgc gcgaagttgt cgtgccccat gccctgcggc agcagccagg
     2101 cgctggcgaa gtaatgctgc accatcgcca cccagccgtc gggcgacggc gtggcgaact
     2161 cggccttgcc cttgtcgatg tcggcaaact cgaccttctg gaacttcttg gcgtcggtgt
     2221 agaaggccgg tccggtgaag gtggaataaa agctggtgcc gcccggcgcc gggttgccgt
     2281 cgcgcaccag ctgcacgtac agctgagggc tgaccggctg gccaccggtg ttgaccacct
     2341 catgccgcac ctgggccacg tagtcgccac gccgcagcgt ataggtcttg accagcttga
     2401 ggccgcccag ctcgggcgac tcgaagcgca gcaccaactc gctcgcacct tcggccaggg
     2461 tgcgctcgcc cgtgaagcgc atcgccgtct tgtgggtggg gaacggccca ccgatcaggc
     2521 ccgactgcgc ctggtaggtg cgctgggggc tgtcgtccag cagcacgaag gcctggcccg
     2581 ggtgttcggt ctggtcgaac ttgaggaact cgctgcgcac gatagagccg ccctcggtgt
     2641 cgagcaccag cttgagcacg tcggtcgtga cttcgatgcg ctcgcgcggc gcagcggcgg
     2701 cggcggccgg tgcggcgtcg ccgggcggca cacccgccgg tgccaccccc ggcaccgcgg
     2761 ccggctgggc gctggccgcc ggcaccgccg acgccggcgc gctggcctgg ctggccggcg
     2821 tgggcgcgcc ggggaagaag gtcgccttgc ggccgttgtg cacctgccac tggtcccaca
     2881 gcatgaccag cgagaaggcg aagatcaccc agaggatggt gcggcgaatg tctttcatga
     2941 cggctgacga ggcgttgacg ggtccgaccg ctcgcggtcg atcagacggg tgaaaaagcg
     3001 cggcggctgc ggcggcaccg gatcgcaacc gccgtcacag ccggggtggc agcgcagcag
     3061 ccggcgcagc gtcaggtagc tgcccgccag cgcgccatgc cgttgcagcg cgccgatggc
     3121 gtagagcgaa caggtcggtt cgaaacgaca ggacgaaccc agccagggcg acagcagcag
     3181 gcgataggcc ttgaccagcc cgatcagcag cgcccgcatc aggaggcgct ccggccctgc
     3241 gcgaacagct ggcacagctc ggcgcgcacc gcccgctgca gcgccaccga cgcggcgctg
     3301 gggtactgcg cgggggcaaa cgccgcccgc aggcgcacca ggtgcgcccc cgcggccagc
     3361 ggcggctgca ggcgctcgcc cacggcgtag atctggcgac ggatcaggtt gcgcgtgacg
     3421 gcgcggcgcg cccagcgctt gggcgccatg gcgcccagcc aggccacgcc ggcaggacag
     3481 aacagccccg gcgcgacggc gtcgggcagg ccctgcggcc ccgccaggcg atgcagggca
     3541 aagtgcgccg tgcgcgccac cggccgctgc gccagcaggg cctggaactg ctcacgcgtg
     3601 cgcaaccggc caggcgtggc cgaccgcagc gcaggcgcag cggtggcgtc gacgccgctc
     3661 accacgagcc gtcccaagcc ggcaggctca gaccgccagg cgcttgcggc ccttggcgcg
     3721 gcgcgcgttg atcaccgcgc gaccgccgcg cgtcttcatg cggaccagaa agccgtgggt
     3781 gcgcgcgcgg cgcgtcttgg aaggttggta cgtgcgtttc atgacgaatc gtccttggag
     3841 taaatgggct tggggtcgtc ccggcccgtc gcggcatctt gggcgccctg cgcgcccgcc
     3901 cggcgcccgg ccgcaccact ggtgcggcca gccccatggc atgccctgcc actggcgcca
     3961 cgcggggaag gcgggctcgc ctgccgcccg gctggcgcgg accccgtggc cccagcgctc
     4021 gcccggcccg acccaggaaa acccgtcatt atcgcaaatt ccgccgtgcc ggccaagggg
     4081 aaggtcatgg catgcgcgcc ggcccgcgac ccgcgggctt tggcagacgc tcttgtggcc
     4141 gcccccgaca tgtggataat tggcgtcgac cgaccctttt cggcgctcct gcggcgccac
     4201 ttatccacac caccagcacc cccatgcaca tcgatcaacc ccagcgcgcc agcgccagcc
     4261 acggcgcggc cttgtgggac acctgcctgg aaacgctggc gcgcgagctg cccgagcagc
     4321 agttcaacac ctggatcaaa ccgctgtcgg cccagctggc ggccgaccac agccgcctga
     4381 ccctctacgt gggcaaccgc ttcaagctgg actgggtgcg cgcccagtac gccacccgca
     4441 tcgccgccgt gctggaggcg ctgcagggcc acgccgtgca ggttgagtta gcgcttgcac
     4501 ccagggcagc gcccatcaaa ccgtcattct ccatcccaac atcggaaacc acccagggcg
     4561 acccggagcc cgcgcgggcg cccgagccct tgcctgcgcc gaccggcgac cgcgacagtg
     4621 ccggcccggt gcgcacccgg ctcaatggcg cgctgacctt cgacaccctg gtggaaggca
     4681 cggccaaccg catggcgcgg gccgccgcgc tgcacgtggc cggcgcgccg ggccagctct
     4741 acaacccgct gttcatctac ggcggcgtcg gcctgggcaa gacgcacctg atgcacgcgg
     4801 tcggcaaccg actgttgcaa acgcgcccgg acgccaaggt tctctacatc catgcggagc
     4861 agttcgtgtc ggatgtggtc aaggcctatc agcgcaagac cttcgatcag ttcaaggaac
     4921 gctaccactc gctcgatctg ctgctgatcg acgacgtgca gttcttcgcc aacaaggacc
     4981 gcacgcagg
//
LOCUS       DAOOOG010000181         4977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_20359_length_4977_cov_37.716172, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000181 DAOOOG010000000
VERSION     DAOOOG010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20359_length_4977_cov_37.716172"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PK242_11390"
     CDS             <1..279
                     /locus_tag="PK242_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOZ94664.1"
                     /translation="DVPTFAEQGYKDDVYMTTAPYYLMAPAKTPDAIVERLGKEVALV
                     VRQPEVQERMEGLGLEPMGDTPAEATALYKDALPKLLKLARDTGVTVD"
     gene            306..1694
                     /locus_tag="PK242_11395"
     CDS             306..1694
                     /locus_tag="PK242_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HOZ94665.1"
                     /translation="MARTPPDLSQARRALARSIGLTAFVYGYPLTETYRTCRKQTDPR
                     DAGKADARNMDARGMRCNTLHHGERPSTDADRDVVTPANDLLYTLSWIHLKDGPQLLT
                     VPSSSAHAGRYFVLALYDAYTENFENLGPRNCGTEGGTVVLVGPHGSVPEALRGHRVV
                     RCPTDLVWLIGRIVVGDESDRSAARRLQADIVLRPAPGTPDGARPAAVEHWRGEPVDA
                     MAAAFENGEPAAQVAPRFFTNLCQAMAEAPGRTQDRGLVAWFGQIGLLPLASFAWEAL
                     DEPAREGLIEGFADGVNLVAAQGRNRRPRPWMIHGATGRYGNEYLGRARVAYLGLGAL
                     ATDEAVYAAAHFDAQQQPLDGRQRYVMQFEAGELPPVDAFWSVTLYDGNRFLYGNEIQ
                     RHSIGDRTPGLHHAADGSLRLEIGHQRPADTRNWLPAPAGRFYLILRMYHPREGFRDW
                     RIPELQPLKDQP"
     gene            1691..>1965
                     /locus_tag="PK242_11400"
     CDS             1691..>1965
                     /locus_tag="PK242_11400"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HOZ94666.1"
                     /translation="MNATERSDAAPSDLAEAAVVYAYPLYEMARMRAATSPRRTESGA
                     EAPAPQRWCNTFVHARQLLRAGGSRVVTPNHDTLYTNAWLDLGAGPLV"
     assembly_gap    1966..2065
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2066..2613
                     /locus_tag="PK242_11405"
     CDS             <2066..2613
                     /locus_tag="PK242_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HOZ94667.1"
                     /translation="PRLRQAQIGRLLPSGWNQPPLIEGHFGNDHLARAQVALKYIGML
                     ESREATYPIAWHDGRGRPLNGAHRYRLRFAPGALPPVDAFWSLTMYDARDYMLVDNPI
                     DRYAIGDRTAGLRHDADGGLTLYLQHAPPMDTAARANWLPAPAGDFYVCLRAYVPREE
                     LLDGRYTLPALECLDETGAPP"
     gene            2610..>3632
                     /locus_tag="PK242_11410"
     CDS             2610..>3632
                     /locus_tag="PK242_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HOZ94668.1"
                     /translation="MNPSDDDKPLLLVGTGSETVAPGLHILRGQGQSFVAETDAGLVV
                     IDAGPGGKVTQGMIDALRQLSDAPVHALCYSHGHIGYNSGVPVWLEHARQRGEPAPRL
                     IAHANLPRRYARYRETQALQHRMAEIQFNRAPGFFDQRLPMHDPTETFGERLVIGSGE
                     QRVELFWAPSETDDAIALWHPGQRLLYGGPALLDSIPNIGTPFRTLRDTVRWADTLEA
                     MAALRPVSAVREFGPAIAGEDEVQRVLLHTAQALRWLRAEVVRLMNEGLNEQQVLAAM
                     HYPDELFAVPWMKPTYGDPSYIVRDIYRSENGWWDRNPTTLHPEPPPAVGEAVAAAIT
                     DKAAVIA"
     assembly_gap    3633..3732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3733..4018
                     /locus_tag="PK242_11415"
     CDS             <3733..4018
                     /locus_tag="PK242_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HOZ94669.1"
                     /translation="AVGEAVAAAITDKAAVIASAQALADAGRWQLALHVIDLLATASG
                     PAPEIAHARRLKAQWLRERARQVPSYVSRNLYRVGADMIEQGTQDRFGIH"
     gene            4040..>4977
                     /locus_tag="PK242_11420"
     CDS             4040..>4977
                     /locus_tag="PK242_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ94670.1"
                     /translation="MTSFALRATCAVVALAASLAVQAQDAASYPIKPVTLVVPTAAGG
                     GTDTIARMFADVLGKALKQPFIIDNRPGANGLLGAENVLGAPADGYRLLFTYTAAMVV
                     NPVLLKKPPFDPLKDFAPVAQIGRGGNIMMVRKDLPVSNLKEFVDYAKARPGKLNYCS
                     WGAGSGGHLTMEVLMKQAGLTMTHVPYKGSMPCNQDLVGGQVDANWSDVSTSIPLVQA
                     GRVKALVSSTPTRLPQLPDVPTLNEAGYPFTTYSWYGVFAPGKTPPAIVAKLNEAIQT
                     ALKDPVVAKRMLELNFTDLPLATPEQFKATVAKDLQ"
BASE COUNT          758 a         1799 c         1581 g          639 t
ORIGIN      
        1 gacgtgccca ccttcgccga gcagggttac aaggacgacg tctacatgac cacggcgccc
       61 tactacctca tggcccccgc caagacgccg gacgccatcg tcgaacgcct gggcaaggaa
      121 gtcgcgctgg tggtgcgcca gcccgaagtc caggagcgca tggaaggcct gggcctggag
      181 cccatgggcg acaccccggc cgaagccacc gcgctctaca aggacgccct gcccaagctg
      241 ctgaagctgg cgcgcgacac cggcgtgacc gtggattgat cccgctcgac acccaggatc
      301 cgacaatggc acgaacccct cccgatctgt cgcaggcccg gcgcgccctg gcgcgctcca
      361 tcggtctgac cgccttcgtc tacggctacc cgctgaccga gacctaccgc acctgccgca
      421 agcagaccga tccgcgcgat gccggcaagg cggacgcgcg caacatggac gcgcgcggca
      481 tgcgctgcaa caccctgcac catggcgaac gcccctccac cgacgccgac cgcgacgtgg
      541 tcacccccgc caacgacctg ctctacacgc tgtcttggat tcacctgaag gacgggccgc
      601 agctgctcac cgtgccgtcg tccagcgcgc acgccggccg ctacttcgtg ctggcgctgt
      661 acgacgccta caccgagaac ttcgagaacc tgggtccgcg caactgcggc accgagggcg
      721 gcaccgtggt gctggtcggc ccgcacggca gcgtgcccga agccctgcgg ggtcaccgcg
      781 tggtgcgctg ccccaccgac ctggtgtggc tgatcggccg catcgtggtg ggcgatgaaa
      841 gcgaccggtc ggcggcgcgc cgcctgcagg ccgacatcgt gctgcgcccg gcccccggca
      901 cgccggacgg cgcgcgcccg gcggccgtcg agcactggag gggcgagccg gtcgatgcca
      961 tggccgccgc cttcgagaac ggcgaacccg ctgcccaggt ggcgccacgc tttttcacca
     1021 acctctgcca ggccatggcc gaagcgcccg gccggacgca agaccgtggc ctggtggcct
     1081 ggttcggcca gatcggcctg ctgcccctgg ccagctttgc ctgggaggcg ctggacgaac
     1141 cggcgcgcga aggcctgatc gagggctttg ccgacggggt caatctggtg gccgcccagg
     1201 gccgcaatcg ccgccccagg ccgtggatga ttcacggcgc caccgggcgc tatggcaacg
     1261 aatacctggg ccgcgcccgc gtggcctacc tgggcctggg ggccctggcc accgacgagg
     1321 cggtgtatgc ggcggcgcac ttcgacgccc agcagcagcc gctcgatggc cggcaacgct
     1381 acgtgatgca gttcgaagcc ggcgagctgc cgccggtgga tgccttctgg tcggtcacgc
     1441 tttacgacgg caaccgcttc ctgtacggca acgagatcca gcgccattcg atcggtgacc
     1501 gcacacccgg cctgcaccac gcggccgacg gcagtctgcg cctggagatc ggccaccagc
     1561 ggcctgccga cacccgcaac tggctgcccg cgccggccgg gcgcttctac ctgatcctgc
     1621 gcatgtacca cccgcgcgaa ggctttcgcg actggcgcat ccccgaattg cagccgctca
     1681 aggaccagcc atgaacgcga cggaacgttc ggacgccgcg ccgtccgacc tggccgaagc
     1741 ggccgtggtt tatgcctacc cgctgtacga gatggcgcgc atgcgcgccg ccacctcgcc
     1801 gcgccgcacc gagtccggcg ccgaggcgcc agcgccccag cgctggtgca acaccttcgt
     1861 gcatgcgcgc cagctgctgc gcgccggcgg cagccgggtg gtcacgccca accacgacac
     1921 gctctacacc aacgcctggc tggacctggg cgcgggcccg ctggtnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnntgccg cgcctgcgcc aggcccagat cgggcgcctg
     2101 ctgcccagcg gctggaacca gcccccgctg atcgaaggcc acttcggcaa cgaccacctg
     2161 gcacgggccc aggtggcgct gaagtacatc ggcatgctgg agagccgcga ggccacctac
     2221 ccgatcgcct ggcacgacgg gcgcggcagg ccgctgaacg gcgcccaccg ctaccgcctg
     2281 cgtttcgcac ccggcgcgct gccgccggtc gatgccttct ggtcgctcac catgtacgac
     2341 gcacgcgact acatgctggt cgacaacccc atcgaccgct acgccatcgg cgaccgcacg
     2401 gccggcctgc gccacgacgc cgacggcggc ctgacgctgt acctgcagca cgcgccaccg
     2461 atggacaccg ccgcgcgcgc caactggctg ccggcgccgg ccggcgattt ctacgtctgc
     2521 ctgcgcgcct acgtgccgcg cgaggaactg ctggacggcc gctacacgct gccggccctg
     2581 gaatgcctgg atgagactgg agccccgcca tgaacccctc cgatgacgac aagccgctgc
     2641 tgctggtggg caccggcagc gaaaccgtgg cgcccggcct gcacatcctg cgcgggcagg
     2701 ggcagtcctt cgtcgccgag accgacgccg gcctggtggt gatcgacgcc ggccccggcg
     2761 gcaaggtgac gcagggcatg atcgacgcgc tgcgccagct gagcgacgcg ccggtgcacg
     2821 cgctgtgcta cagccatggc cacatcggct acaactcggg cgtgccggtg tggctggagc
     2881 acgcacgcca gcgcggcgag ccggcgccgc gcctgatcgc gcacgccaac ctgccgcgcc
     2941 gctacgcgcg ctaccgcgag acccaggccc tgcagcaccg catggccgag atccagttca
     3001 accgggcgcc gggattcttc gaccagcgcc tgcccatgca cgatccgacc gagacctttg
     3061 gcgagcgcct cgtcatcggc agcggcgagc agcgggtcga gctgttctgg gcaccctccg
     3121 agaccgacga cgccatcgcc ctgtggcacc ccggccagcg cctgctgtac ggcggcccgg
     3181 ccctgctcga ctcgatcccc aatatcggca cgccgtttcg caccctgcgc gacacggtgc
     3241 gctgggccga cacgctggag gccatggcgg cgctgcgccc ggtctcggcg gtgcgcgaat
     3301 ttggcccggc gattgccggc gaggacgagg tgcagcgcgt gctgctgcac acggcccagg
     3361 cgctgcgctg gctgcgcgcc gaggtggtgc ggctgatgaa cgaaggcctg aacgagcagc
     3421 aggtgctggc cgccatgcac taccccgacg agctgtttgc cgtgccctgg atgaagccca
     3481 cctacggcga ccccagctac atcgtgcgcg acatctaccg gtcggagaac ggttggtggg
     3541 accgcaaccc aaccacgctg caccccgagc caccgccggc ggtgggcgag gccgtggcgg
     3601 ccgccatcac cgacaaggcg gcggtgatcg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnggcggtgg gcgaggccgt ggcggccgcc atcaccgaca aggcggcggt
     3781 gatcgccagt gcccaggcgc tggccgacgc cggccgctgg cagctggcgc tgcacgtgat
     3841 cgacctgctg gccaccgcca gcggcccggc acccgagatc gcgcacgcgc gccggctcaa
     3901 ggcccaatgg ctgcgcgagc gcgcccggca ggtgcccagc tacgtgtcac gcaacctgta
     3961 ccgcgtgggc gccgacatga tcgagcaggg cacgcaggac cgctttggca ttcattgacc
     4021 atttcaacag gagaccacca tgacctcttt cgccctgcgc gccacctgcg ccgtcgtcgc
     4081 ccttgccgcc agcctggcgg tgcaagccca ggacgccgcc agctacccga tcaagccggt
     4141 caccctggtg gtgcccacgg cggccggtgg cggcaccgac accatcgcgc gcatgttcgc
     4201 cgacgtcctg ggcaaggcgc tgaagcagcc cttcatcatc gacaaccgcc cgggcgccaa
     4261 cggcctgctg ggtgccgaga acgtcctcgg cgcgccggcc gatggctatc ggctgctgtt
     4321 cacctacacc gcggcgatgg tggtcaaccc cgtgctgctg aagaagcccc cgttcgatcc
     4381 cctgaaggac ttcgctccgg tggcgcagat cggccgcggc ggcaacatca tgatggtgcg
     4441 caaggacctg ccggtcagca acctcaagga attcgtcgac tacgccaagg cgcgccccgg
     4501 caagctcaac tactgctcct ggggcgccgg ctcgggcggg cacctgacca tggaggtact
     4561 gatgaagcag gcgggcctga ccatgaccca cgtcccgtac aagggcagca tgccgtgcaa
     4621 ccaggacctg gtcggcggtc aggtggacgc caactggtcg gatgtctcga ccagcattcc
     4681 gctggtccag gccggccggg tgaaggcgct ggtcagctcg accccgaccc gcctgccgca
     4741 gctgcccgac gtgccgacgc tgaacgaagc cggctacccg ttcaccacct actcctggta
     4801 cggcgtgttc gcgcccggca agacgccgcc ggcgatcgtc gccaagctga acgaagcgat
     4861 ccagaccgca ttgaaagacc cggtcgtcgc caagcgcatg ctcgagctga acttcaccga
     4921 cctgccgctc gccacgccgg agcagttcaa ggccacggtg gccaaggatc tgcagga
//
LOCUS       DAOOOG010000182         4914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_20674_length_4914_cov_24.145915, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000182 DAOOOG010000000
VERSION     DAOOOG010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20674_length_4914_cov_24.145915"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /gene="ettA"
                     /locus_tag="PK242_11425"
     CDS             complement(<1..1214)
                     /gene="ettA"
                     /locus_tag="PK242_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOZ94671.1"
                     /translation="MAQYVFSMNRVGKIVPPKRHILKDISLSFFPGAKIGVLGLNGSG
                     KSTLLKIMAGIDKDIEGEAVPMPGLDIGYLPQEPQLDPAHTVRQEVELAMGEVNAARA
                     RLEEIYAAYAEPDADFDALSEEQGKLEAVIAAAGTDSEHQLEIAADALRLPPWDAVIG
                     QLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEIFLKRFPGTVVAITHDR
                     YFLDNAAEWILELDRGQGIPWKGNYSTWLEQKEARLEQEKKSEDARMKAMKKELEWAR
                     QNPKGRQAKSKARLARFEELSDYEYQKRNETQEIFIPVAERLGTEVIEFKSVSKSFGD
                     RLLIDNLSFKMPAGAIVGIIGPNGAGKSTLFKLIAGREQPDSGEVVIGKTVQLAFVDQ
                     SREALADDKTVW"
     gene            1476..1994
                     /gene="xrtH"
                     /locus_tag="PK242_11430"
     CDS             1476..1994
                     /gene="xrtH"
                     /locus_tag="PK242_11430"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HOZ94672.1"
                     /translation="MKRFFLIFLTVQAVLFGIEMLNPVQTAVVHPWTGWLAKVSAAIV
                     HLIDPTVISYGRVLQSAKTGFGVSIEAGCNGVEATIVLVAAMLAFPSSWAMKAWGIGL
                     GFIAVQAVNLLRIVSLFFLGQWNFKVFEFAHLYMWQALIMLDVLVVWLLWMRYVAKRD
                     PQPAAGASAAAS"
     gene            2008..2643
                     /locus_tag="PK242_11435"
     CDS             2008..2643
                     /locus_tag="PK242_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94673.1"
                     /translation="MRNTLIGRFLLLSILWLALLLPLWYWAARWLAMPVIWLAGTVMQ
                     ALFPWVDGWAQEGVSAVLHTLVQVRMPGAQGDVLGELAPEASYPTYGYGLVLLWAMLL
                     ASRTERWWLKGLIGSLLLIPAQAWGICFQWLRDVVLMSGTYGAAYLKWSPLAQNAVAY
                     GYQFGFLMLTPVAPILLWLLFNKRFVAALWLEAALEAKPDPRAQAPTRSVE"
     gene            complement(2929..4275)
                     /locus_tag="PK242_11440"
     CDS             complement(2929..4275)
                     /locus_tag="PK242_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HOZ94674.1"
                     /translation="MPLPFRRPRARLALLLLWGATTAQAQSLLQLYQTAHGYDATYRS
                     ALANAQASQARADQARAGLLPQLGMQAGAQHNWADTHVLGGGSSRSFNTLNAALVGSQ
                     PLYRPANRIAWDQGQRAAEAAQINLVGAEQDLIVRLAQAYFDVLAAQDALASVRALKA
                     AVNEQLQAARRNFEVGTATITDTREAQARFDLATAQEIATENDLRVRKLALDQLVGRS
                     GSEPRPLAQPITLPAPQPDDVTAWVERAAEQHPGVRQARAALDIARLETDKARTGHRP
                     TVDLQASVGQTRYPDGNPSLSAAPRASARTTNASIGVVLNWPLFAGFAVENRIRETLS
                     LEDRAQADLDQLQRSVSQATRAAFFGVQSGLGQVKALQAAEASSQTALQANQLGYQVG
                     VRINIDVLNAQSQLYQTQRDLARARYDVLVGTLRLKQAAGTLSQDDLIPINQLLVP"
     gene            complement(4286..4627)
                     /locus_tag="PK242_11445"
     CDS             complement(4286..4627)
                     /locus_tag="PK242_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOZ94675.1"
                     /translation="MIEQVSPRDLASWLRAAGGDRQPLLLDVREPQEWRLASVQIDGA
                     ELQQMAMHTIAPRLHELDPARPVAVLCHHGGRSMQVAMFLAQRGFARVANVAGGIDAW
                     ALQLDPALPRY"
     gene            complement(4661..>4914)
                     /locus_tag="PK242_11450"
     CDS             complement(4661..>4914)
                     /locus_tag="PK242_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOZ94676.1"
                     /translation="ARTDLSAHGPFDVIVLSGSVAELPDALLALLKPGGRLMAIVGHE
                     PVMRATLVLRTDNGLVSSQPWDSNAPRLCHFPEPSRFQF"
BASE COUNT          672 a         1586 c         1753 g          903 t
ORIGIN      
        1 tcccacaccg tcttgtcgtc ggccagcgcc tcgcggctct ggtcgacgaa ggccagctgc
       61 acggtcttgc cgatcaccac ctcgccgctg tcgggctgct cgcgaccggc gatcagcttg
      121 aacagcgtgg acttgccggc gccgttggga ccgatgatgc cgacgatggc gccggccggc
      181 atcttgaaac tcaggttgtc gatcagcagg cggtcaccga agctcttgct gacgctcttg
      241 aactcgatca cttccgtgcc caggcgttcg gccaccggga tgaagatttc ctgcgtctcg
      301 ttgcgcttct ggtattcgta gtcgctcagc tcctcgaagc gcgccagccg cgccttgctc
      361 ttggcctggc gacccttggg gttctggcgc gcccattcca gctccttctt catggccttc
      421 atgcgcgcgt cttcgctctt cttttcctgt tccaggcgcg cttccttctg ttccagccag
      481 gtggagtagt tgcctttcca gggaatgccc tggccgcggt cgagctccag gatccactcg
      541 gcggcgttgt ccagaaagta acggtcgtgc gtgatggcca ccacggtgcc gggaaagcgc
      601 ttgaggaaga tttccagcca ttccacgctt tcggcgtcca ggtggttggt gggttcgtcc
      661 agcagcagca tgtcgggctt ggacagcagc agcttgcaca gcgccacgcg gcgcttttcg
      721 ccgccggaga gctggccgat cacggcgtcc cagggcggca ggcgcagcgc gtcggcggcg
      781 atctccagct ggtgctcgct gtcggtgccg gcggcggcga tcaccgcctc cagcttgccc
      841 tgctcttcgg agagggcgtc gaagtcggcg tccggttcgg cgtaggcggc gtagatttcc
      901 tccagccggg cgcgcgccgc gttgacttca cccatggcca gttcgacctc ctggcgcacg
      961 gtgtgcgcgg ggtcgagctg cggctcctgc ggcaggtagc cgatgtccag gccgggcatc
     1021 ggcacggctt cgccctcgat gtccttgtcg atgccggcca tgatcttcag caaggtggac
     1081 ttgcccgagc cgttgaggcc cagcacgccg atcttggcgc cgggaaaaaa cgacagcgag
     1141 atgtccttca agatgtgtcg cttgggcggc acgatcttgc cgacgcggtt catggaaaaa
     1201 acgtactggg ccatagatta ctcttgaatt gatagctgat ggcgcacgcc agtcgtgcac
     1261 cagaggctga aaacacctga aatatttcga aggtgggtgc ccgctcggtg ggtggcgggc
     1321 aaagaagcgg caggggccat tatcgaccgg gcgccccgtg gaccccgcat ggaccctcca
     1381 ctagcccgag gatacaagcc gctacactgt gagccagggc agtggcgcat caggtgccac
     1441 ggcgcactgg agttgtcaag gattcgggcc gtaccgtgaa gcgctttttt ctgattttcc
     1501 tgaccgtgca ggcggtgttg ttcggcatcg agatgctcaa tccggtgcag acggccgtgg
     1561 tgcatccgtg gaccggctgg ctggccaagg tcagtgccgc catcgtgcac ctgatcgacc
     1621 cgacggtgat ttcgtatggc cgcgtgctgc aaagcgccaa gaccggcttc ggggtgtcga
     1681 tcgaggccgg ctgcaatggc gtcgaggcca ccatcgtgct ggtggcggcg atgctggcct
     1741 ttccttccag ctgggccatg aaggcctggg gcatcggcct gggcttcatc gccgtgcagg
     1801 cggtcaacct gctgcgcatc gtcagcctgt tcttcctggg gcagtggaac ttcaaggtgt
     1861 tcgagttcgc ccacctgtac atgtggcagg ccctgatcat gctggacgtg ctggtggtgt
     1921 ggctgctgtg gatgcgctac gtcgccaagc gcgacccgca accggcggcc ggcgcatcgg
     1981 ccgcggcgtc gtgacgggag cctggccatg cgcaacacgc tgatcggtcg ctttctgttg
     2041 ctgtcgatcc tgtggctggc cctgctgctg ccgctgtggt actgggccgc gcgctggctg
     2101 gcgatgccgg tgatctggct ggccggcacg gtgatgcagg cgctgtttcc gtgggtcgac
     2161 ggctgggcgc aggaaggcgt cagcgcggtg ctgcacaccc tggtgcaggt gcgcatgccg
     2221 ggcgcgcagg gcgatgtgct gggcgagctg gcaccggagg ccagctaccc gacctatggc
     2281 tatggcctgg tgctgctgtg ggccatgctg ctggcttcgc gcaccgagcg ctggtggctc
     2341 aaggggctga tcggcagtct gctgctgatt ccggcccagg cctggggcat ctgctttcag
     2401 tggctgcgcg acgtggtgct gatgtccggc acgtacggcg cggcgtatct gaaatggtcg
     2461 ccactggccc agaacgccgt ggcctacggt taccagttcg gctttctgat gctgacgccg
     2521 gtggccccca tcctgctgtg gctgctgttc aacaagcgct tcgtggccgc actgtggctg
     2581 gaggccgcgc tggaagccaa acccgacccg cgggcccagg caccgacccg gtcggtcgag
     2641 tgaatggcta tctttttgat agcttatggc gcacgacagg caagcgtcgt ggccactttt
     2701 ggctcggata cggttgcgcg atcagaactg tgacccaagt accggctgcg ccggcatgac
     2761 cgctgaccca tgggtcatcg aagcgcagcg aggccatccg tcatgggtca ttgtgcaggc
     2821 aagttggtat cggccggtgg cgcagcgcgc ccacccgctg gccggccttc aggcctggcc
     2881 gcgcagcgcg tcgatgaccg cggtgtcggc gctcaccgcg gcgccgcatc agggcaccag
     2941 cagctggttg atcgggatca ggtcgtcctg cgacagcgtg ccggcggcct gcttcaggcg
     3001 cagcgtgccc accagcacgt cgtagcgcgc gcgcgccagg tcgcgctgcg tctgatagag
     3061 ctggctttgc gcgttcagca cgtcgatgtt gatgcgcacc ccgacctggt agcccagctg
     3121 gttggcctgc agcgcggtct ggctggaggc ttcggccgcc tgcagcgcct tgacctgccc
     3181 gaggcccgat tgcacaccga aaaacgccgc ccgcgtggcc tgtgacacgc tgcgctgcag
     3241 ctggtccaga tcggcctggg cgcggtcttc cagcgacagg gtctcgcgga tgcggttttc
     3301 caccgcgaac ccggcaaaca acggccagtt cagcaccacg ccgatgctgg cgttggtggt
     3361 gcgcgcgctg gcgcgcggcg ccgccgacag gctggggttg ccatcggggt agcgggtctg
     3421 gccgacgctg gcctgcaggt ccaccgtggg ccggtggccg gtgcgggcct tgtcggtttc
     3481 caggcgcgcg atgtccagcg ccgcgcgggc ctgacgcacg ccggggtgct gctcggcggc
     3541 ccgttcaacc caggcggtga cgtcgtccgg ctgcggcgcc ggcagcgtga tgggctgtgc
     3601 cagcggccgc ggttcgctgc cgctgcggcc caccagctgg tcgagcgcga gttttctgac
     3661 gcgcagatcg ttttcggtag cgatctcctg cgccgtggcg aggtcgaagc gcgcctgcgc
     3721 ctcgcgggtg tcggtgatgg tggcggtgcc gacctcgaag ttgcgccgcg cggcctgcaa
     3781 ttgctcgttc acggcggcct tcagagcgcg caccgacgcc agcgcgtcct gcgccgccag
     3841 cacgtcgaaa taggcctgcg ccagccgcac gatcaggtcc tgctcggcac cgaccagatt
     3901 gatctgggcg gcctcggcgg cgcgctggcc ctggtcccag gcgatgcggt tggccggccg
     3961 atacaggggc tgggagccga ccagggcggc gttcagggtg ttgaagctgc ggctgctgcc
     4021 gccgcccagc acatgggtgt cggcccagtt gtgctgcgct ccggcttgca tgcccagctg
     4081 cggcagcagg ccggcgcggg cctggtcggc gcgcgcctga ctggcctggg cgttggccag
     4141 cgccgagcgg taggtggcgt cgtagccgtg cgcggtctgg tacagctgca gcagggactg
     4201 cgcctgcgcg gtcgtggcac cccagagcag cagcagggcc aggcgggccc gggggcggcg
     4261 aaacggcagc ggcatcggcg gtccttcagt agcggggcag ggcggggtcg agttgcaagg
     4321 cccaggcgtc gatgccgccc gccacgttgg ccacgcgggc aaagccgcgc tgcgccagga
     4381 acatggccac ctgcatgctg cgcccgccgt ggtggcacag caccgccacc gggcgcgcgg
     4441 ggtcgagttc gtgcaggcgt ggcgcaatgg tgtgcatggc catctgctgc agttcggcgc
     4501 cgtcgatctg gacgctggcc aggcgccatt cctggggttc gcgcacgtcc agcagcagcg
     4561 gctggcggtc gccgccggcg gcccgcagcc agcttgccag atcgcgcgga gatacttgtt
     4621 caatcatcgt gcagcgcttg tcctgcttgc gccaggcgct tcagaattgg aagcgcgaag
     4681 gctccgggaa gtggcacagg cgcggcgcgt tgctgtccca gggctggctg ctgacgaggc
     4741 cgttgtcggt gcgcagcacc agggtggcgc gcatcaccgg ctcgtggccc acgatggcca
     4801 tcagccgtcc gccgggcttg agcagcgcca gcagggcgtc gggcagctcg gccaccgaac
     4861 cggacagcac gatgacgtcg aacgggccgt gcgccgacag gtcggtgcgg gcgc
//
LOCUS       DAOOOG010000183         4912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_20684_length_4912_cov_30.086061, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000183 DAOOOG010000000
VERSION     DAOOOG010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20684_length_4912_cov_30.086061"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PK242_11455"
     CDS             complement(<1..904)
                     /locus_tag="PK242_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOZ94677.1"
                     /translation="MTLTELKYIVAVAREKHFGKAAEACFVSQPTLSVAVKKLEEELE
                     VKLFERGGSEIAVTPLGAEIVRQAQSVLEQAAEIKEIAKRGKDPLGGPLTLGVIYTIG
                     PYLLPELVRHNIERTPQMPLMLQENFTVRLLEMLRTGEIDCAILAEPFPDSGLAVAPL
                     YDEPFMAALPAAHPLARGAAVTSEQLRGEHMLLLGTGHCFRDHVLQVCPEFAGFASSG
                     EGIRKNFEGSSLETIKHMVAAGMGVTLVPRLAVPAEALVAQAAQADAPGAVRYLPVRD
                     GGGAAPPTRRVVLAWRRSFTRYEAI"
     gene            complement(1043..1777)
                     /locus_tag="PK242_11460"
     CDS             complement(1043..1777)
                     /locus_tag="PK242_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ94678.1"
                     /translation="MTSASPAAAAASSLIRRALGLCRMLRGWPGEVLDQLAAVSWVGR
                     YQRHEQIMADDPQRREVLVVASGCIAVDHIDASGQHFLLSMYGPGDITSLIRLLTDAP
                     FHFGFHAREPSTVVHLPCDAMMTVLDAHPILWKDLCLLMLARTQQSILLQQQRSSGQM
                     KQMVADAVVQLVRARGLDTAAPGAVCVRVSQTELAAMLSVSRQTVNKVLQALCQAGLL
                     RVHYGRLEGINLGRLERVARGEPEPH"
     gene            1965..4082
                     /locus_tag="PK242_11465"
     CDS             1965..4082
                     /locus_tag="PK242_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOZ94679.1"
                     /translation="MEHFNFTQLDTLFAPRSIAVVGASEQTTKIGGIPLDYLRRFGYS
                     GRLHAVNPRARSVQGLPAHESLRTIGAPVDMAIVAVPQAQVAAVLDDAADAGVRTMVL
                     FSSGYAEVGAEGAAQQQALAARAQAAGIRLLGPNCLGIMRPSHNVYATFAPAVNAGLV
                     KPGRIGLISQSGAFGVYAYSLARERGLGLSLWATTGNEADVQLADGLAWLAQDPETDV
                     IMAYMEGCRDGPRLRAALALAQQRGKPVVMVKVGASALGAAAAASHTASLAGDDAVYD
                     AVFRRYGVLRVHSISEFFALAASAAIARPPKGRSIGLMTVSGGVGALMADDASAAGLE
                     VPPLSAAAQRQLLDWVPFAGPRNPVDVTAQVTNDDTLLERSATLMLNDQPFHSWLGFF
                     AAAGLSPTVWPAIVGLVRTLRARHPDTLLAVSTLCSDERRAELEALGCLVFADPGIAI
                     RCIGALTRWGTQGAPAQAPALTAARLHVPPGTLSEADSLALLAAAGVPLVEHRLVHTP
                     NQAAAAVDALGGAIALKVASADIPHKSDAGGVMLRLGSADQARAAFERITHNVRQAHP
                     DARIDGALAARMVTGGVECIAGVHRDPVFGPVLMFGLGGVHVELLRDVSLRVLPIGRD
                     EARAMVMELRAAAIFHGVRGQPAVDVESIAEVLCALAEFAMRAGDSLVSAEINPLIAR
                     PAAEGGAIAVDALVIGRAATGAG"
     gene            4138..>4912
                     /locus_tag="PK242_11470"
     CDS             4138..>4912
                     /locus_tag="PK242_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ94680.1"
                     /translation="MSRTHRHARWLRALGLSVSLLLPLTGSWAQSAYPNKPVRIVVQF
                     AAGGGSDTLARAIADGLSRRLGQPVIVENKAGAGGIIGADHVAKAAPDGYTVLLTIPG
                     AVTSFKAIYKKLPYDPETDLRLISDIALPRTVLAVNPSVPAQTYKELLDLVKKAPGKF
                     TMGSWGPGVQPHQLQVFMDKTYGTQTALAAYKGEAPMAMDLISGVIPMAVGSTAALKP
                     HITAGKLRPLAVFGSTRTKALPDVPTFAEQGYKDDVYMTT"
BASE COUNT          729 a         1739 c         1718 g          726 t
ORIGIN      
        1 cgatggcttc gtagcgggtg aagctgcggc gccaggccag caccacgcgg cgcgtcggcg
       61 gtgcggcgcc gccgccgtcg cgcaccggca ggtagcgcac cgcgccgggc gcgtcggcct
      121 gcgcggcctg ggccaccagc gcctcggccg gcaccgccag ccgcggcacc agggtcacgc
      181 ccatgccggc ggccaccatg tgcttgatgg tttcaagcga cgagccttcg aagttcttgc
      241 ggatgccttc gccactgctg gcgaagccgg cgaattcggg acagacctgc agcacatggt
      301 cgcgaaagca gtgaccggtg cccagcagca gcatgtgttc gccgcgcagc tgctccgacg
      361 tgacggcggc gccgcgcgcc agcgggtgag cggccggcag cgcggccatg aagggctcgt
      421 cgtacagcgg cgccaccgcc agccccgagt caggaaaggg ttcggccagg atggcgcagt
      481 cgatctcgcc ggtgcgcagc atttccagca ggcgcacggt gaagttttcc tgcagcatca
      541 gcggcatctg cggcgtgcgc tcgatgttgt ggcgcaccag ctcgggcagc aggtaggggc
      601 cgatggtgta gatcacgccg agcgtcagcg gcccgcccag cgggtccttg ccgcgcttgg
      661 cgatttcctt gatctcggcg gcctgctcca gcacgctctg ggcttgacgc acgatttcgg
      721 cgcccagcgg ggtgacggcg atctcgctgc cgccgcgctc gaacagtttg acctccagct
      781 cctcttccag cttcttgacg gccaccgaca gcgtgggctg cgacacgaag caggcctcgg
      841 ccgccttgcc gaagtgcttt tcgcgcgcca cggcgacgat gtatttgagt tcggtcaggg
      901 tcatggcagc tccaggcaag ggttggcgac ccgcacggcc ggagcgccgc gacgggccgg
      961 cggccggcgc gccgccccga cacgatagcg ccagagcgtg ccgcaggccg ggcggccggc
     1021 gcagggcacg gcgtgtgcca ggtcagtgcg gctcaggttc accccgcgcc acccgctcga
     1081 gtcggcccag gttgatgccc tccagccgtc catagtggac gcgcagcaga cccgcctgac
     1141 acagagcctg cagcaccttg ttgacggtct ggcgcgacac cgacagcatg gccgccagct
     1201 cggtctgcga cacccgcaca cacaccgccc cgggcgccgc agtgtccagg ccgcgcgcgc
     1261 gcaccagttg gaccaccgcg tccgccacca tctgcttcat ctgaccactg gagcgctgct
     1321 gctgcagcag gatgctttgc tgggtgcgcg ccagcatcag caggcacagg tccttccaga
     1381 ggatggggtg ggcgtcgagc acggtcatca tggcgtcaca gggaagatgc accacggttg
     1441 acggctcgcg ggcatgaaag ccgaagtgaa acggcgcgtc ggtcagcagg cgaatcagcg
     1501 aggtgatgtc gcccgggccg tacatggaca gcagaaagtg ctgacccgag gcgtcgatgt
     1561 ggtcgacggc aatgcaaccc gaagccacca ccagcacctc gcgccgctgc ggatcgtcgg
     1621 ccatgatctg ctcgtggcgc tggtagcgcc cgacccagga cacggcagcc agctgatcga
     1681 gcacctcgcc cggccagcca cgcagcatcc ggcacagccc cagcgcgcga cggatcaggc
     1741 tcgacgccgc ggcagcggcc ggcgacgctg acgtcattgc tgggccactg cggcccgggg
     1801 ctgtgtccat agggtttcca cgcaatccca atcggcctca attgtcaatc acttgacaga
     1861 cgctgcggtg cccagcttcg catagtacgg atcagtcggc tgacgacgcg tcgcaccgat
     1921 cgcgcgcagg gccgggccgg caatgccacc ccacgacacg gagcatggag cacttcaatt
     1981 tcacccagct cgacacgctg tttgcgccgc gctcgatcgc ggtggtgggc gcgtccgagc
     2041 agaccaccaa gatcggcggc atcccgctcg attacctgcg gcgtttcggc tacagcggcc
     2101 ggctgcacgc ggtgaatccg cgcgcgcgca gcgtgcaggg cctgcccgcg cacgagagtc
     2161 tgcgcaccat cggcgcgccg gtggacatgg ccatcgtggc cgttccgcag gcccaggtgg
     2221 cggccgtgct ggacgacgcg gcggacgccg gcgtgcgcac catggtgttg ttttcctccg
     2281 gctatgccga ggtcggcgcc gagggcgcgg cgcagcagca ggcgctggcg gcgcgcgcgc
     2341 aggcggccgg tattcgcctg ctgggcccca actgcctggg catcatgcgg ccgagccaca
     2401 acgtctacgc caccttcgcg ccggcggtga acgccggcct ggtcaagccc gggcgcatcg
     2461 gcctgatcag ccagtcgggc gcgttcggcg tctacgccta cagcctggcg cgcgagcgcg
     2521 gcctgggcct gtcgctgtgg gccaccaccg gcaacgaggc cgacgtgcag ctggccgacg
     2581 gtctggcctg gctggcacag gacccggaga ccgacgtcat catggcctac atggaaggct
     2641 gccgcgatgg cccacgcctg cgcgctgcgc tggcgctggc gcagcagcgc ggcaagccgg
     2701 tagtgatggt caaggtcggc gccagcgcac tgggcgcggc ggcagcggcc tcgcacaccg
     2761 cctcgctggc cggcgacgat gcggtctacg acgccgtgtt ccggcgctac ggtgtgctgc
     2821 gcgtgcacag catcagcgag ttttttgcgt tggccgccag cgccgccatt gcccgcccgc
     2881 ccaaggggcg ctcgatcgga ttgatgacgg tctccggcgg cgtcggcgcc ctgatggccg
     2941 acgacgccag cgccgccggg ctggaggtgc caccgctgtc ggccgccgca cagcgccagc
     3001 tcctcgactg ggtgccgttt gccggcccgc gcaaccctgt cgacgtcacc gcccaggtga
     3061 ccaacgacga caccctgctg gagcgcagcg ccacgctgat gctgaacgat cagccgtttc
     3121 acagctggct gggcttcttc gcggcagcgg ggctttcacc tacggtctgg cctgccatcg
     3181 tgggcctggt gcgaaccctg cgcgcgcgcc atccggacac cttgctggcg gtcagcacgc
     3241 tgtgcagcga cgagcgccgc gccgagctgg aggccctggg ctgcctggta tttgccgacc
     3301 cgggcatcgc catccgctgc atcggggcgc tgacccgctg gggcacccaa ggcgccccgg
     3361 cgcaggcccc cgccctgacc gctgcccgcc tgcacgtgcc accgggcacg ctgtccgagg
     3421 ccgacagtct ggcgctgctg gccgccgccg gggtgccgct ggtcgagcac cggctggtcc
     3481 acacaccgaa tcaggccgcc gccgccgtgg atgccttggg tggcgccatc gcactgaagg
     3541 tcgccagcgc cgacatcccc cacaagtcgg acgccggcgg cgtgatgctt cggctgggca
     3601 gcgccgacca ggcgcgcgcc gctttcgagc gcatcacgca caacgtccgc caggcccacc
     3661 ccgatgcccg catcgatggc gcgctggcgg cccgcatggt gacgggcggc gtggaatgca
     3721 tcgccggtgt gcaccgcgac ccggtgttcg ggccggtgct gatgttcggg ctgggcggcg
     3781 tgcacgtcga gctgctgcgc gacgtgtcgc tgcgtgtgct gccgatcggt cgcgacgagg
     3841 cccgtgccat ggtgatggag ctgcgcgcag cggccatctt tcacggcgtg cgcgggcagc
     3901 cggcggtcga tgtggaatcg attgccgagg tcctgtgcgc gctggccgag ttcgccatgc
     3961 gtgccggtga ctctctggtc agcgccgaga tcaacccgct gatcgcccgc ccggcggcgg
     4021 aaggcggtgc gatcgccgtc gacgccctgg tgatcggtcg cgccgcgacg ggtgcgggct
     4081 gagtgccggc gcctttcgct ttggtttgcc tgaacctgga tatctgagga gatgaagatg
     4141 agccgaaccc acagacacgc ccgctggctg cgcgccctgg gcctgagtgt gagcctgctg
     4201 ctaccgctga ccggcagctg ggcacagagc gcctatccga acaagccggt gcgcatcgtg
     4261 gtgcagtttg cggctggagg cggcagcgac acgctggcgc gcgccatcgc cgacgggctg
     4321 tccaggcgac tcgggcaacc ggtcatcgtg gagaacaagg ccggcgcggg cggcatcatc
     4381 ggcgccgacc atgtggcgaa ggcggcgcct gacggctata ccgtgctgct gacgattccc
     4441 ggcgcggtca cctccttcaa ggcgatctac aagaagctgc cctacgaccc ggagaccgac
     4501 ctgcgcctga tctcggacat cgcgctgccg cgcaccgtgc tggccgtcaa cccgtcggtg
     4561 ccggcccaga cctacaagga gctgctcgat ctggtcaaga aggcacccgg caaattcacc
     4621 atgggctcct gggggccggg cgtgcagccg caccagctgc aggtcttcat ggacaagacc
     4681 tacggcaccc agacggcgct ggccgcgtac aagggtgagg cccccatggc catggatctg
     4741 atcagcggcg tgatcccgat ggcggtgggc tcgaccgccg cgctcaaacc ccacatcacc
     4801 gccggcaagc tgcgcccgct ggcggtgttc gggtcgaccc gcaccaaggc gctgcctgac
     4861 gtgcccacct tcgccgagca gggttacaag gacgacgtct acatgaccac gg
//
LOCUS       DAOOOG010000184         4882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_20860_length_4882_cov_27.849596, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000184 DAOOOG010000000
VERSION     DAOOOG010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20860_length_4882_cov_27.849596"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PK242_11475"
     CDS             <1..569
                     /locus_tag="PK242_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOZ94681.1"
                     /translation="RVIGKHRKLMPTALERLVWGFGDGSTLQAVDTPWGKLGAVICWE
                     NYMPALRLAMYQQRVAIYCAPTADDRDSWQSTMQHIALEGRCFVLSACQHLRRRDFPG
                     ERMNNRLPEAPDTVLMRGGSSIHDPLGQRLAGPVFGEDALLTAELDLSIIPAAQMDFD
                     PVGHYARPDVFSLHVDTAPQRAVQLGDQ"
     gene            569..1492
                     /gene="hpaD"
                     /locus_tag="PK242_11480"
     CDS             569..1492
                     /gene="hpaD"
                     /locus_tag="PK242_11480"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HOZ94682.1"
                     /translation="MGQLALAAKITHVPSMYLSELPGARQGTRQDAIDGHKEIGRRCR
                     ELGVDTMVVFDTHWLVNATYHLNCAPRWQGSYTSNELPHFISNMAYGFNGNPELGRLL
                     AKACNEQGVETLAHDATSLQPEYGTLVPMRYMNADEHFKVVSVSALCTSHYLNDSARL
                     GWAMREAVEKHYDGKVAFMASGSLSHRFAQNGLAPEFAFKIWSPLLERLDHEVIQMWK
                     NGEWPDFCAMLPEYATKGHGEGMMHDTAMLLGALGWSAYDGKMEVITPYFAASGTGQM
                     NAVFPVTPQDGHAVPPPEASRATGFRAFARI"
     gene            1505..1963
                     /locus_tag="PK242_11485"
     CDS             1505..1963
                     /locus_tag="PK242_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HOZ94683.1"
                     /translation="MPHLVILYSGNLDAIVDMPALCRDAADAMLAVRDEAGAQVFPTG
                     GTRVLAYPAPHFALADGGAAGRAAGAGKTGITGSDPGEYGFMYANLRMARGRSSATQQ
                     AAGEAIARALRARLDPCMATRHIGLTVQVDEGPEVFDAKHSTLHPLFRKA"
     gene            1965..2780
                     /gene="hpaH"
                     /locus_tag="PK242_11490"
     CDS             1965..2780
                     /gene="hpaH"
                     /locus_tag="PK242_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HOZ94684.1"
                     /translation="MLTDEQIQQLAAELHASEKSLQQVEHFSKRFPGMTIEDGYRISR
                     AWVALQRAEGRQVIGHKIGLTSRAMQISSQIDEPDYGTLLDNMLYTCTPGQVLPIPTD
                     RFIAPRVEVELAFVLKADLAGPHVDVEQVLAATDYVTPAIEIIDARIEQFDRHTRVMR
                     KVYDTISDNAANAGIVVGAGRADARAIDRAWVGAILRQNGAVEETGLAAGVQGDPAIG
                     IAWLANKLAPWGEVLRARQIVLAGSFTRPVAAKRGDVFEADYGPLGQLQFKFV"
     gene            2783..3574
                     /locus_tag="PK242_11495"
     CDS             2783..3574
                     /locus_tag="PK242_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HOZ94685.1"
                     /translation="METPLNPFKQALHEKRRQIGFWLNLPNTTVAEIAAGAGFDWLLI
                     DGEHTYHSLETILHQLQTIAAYPRTHAIARLPMGHGHVGEMLIKQYLDLGVQTLLVPM
                     VDTPEQAAAIVRAARYPGGGGAGGIRGMAGGRAARWGRYPKYVHQANDRVCILVQAET
                     RTALDNLEAIAAVDGVDGIFIGPADLSASMGHRGNPAHPEMQAIMEDATRRIVRAGKA
                     AGNLSPDAATAQRYLEWGATFVAVGLDTNVLARETSALAQKFAAG"
     gene            3623..4471
                     /gene="yghU"
                     /locus_tag="PK242_11500"
     CDS             3623..4471
                     /gene="yghU"
                     /locus_tag="PK242_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HOZ94686.1"
                     /translation="MSSAYVPPKVWRWEPKEDNKFANINRPTAGARFEQELPVGQHPL
                     QLYSLATPNGQKVTILLEELLAAGHAGAEYDAWLIDIGEGAQFGSGFVAINPNSKIPA
                     LLDRSVQPAIPVFESGAILLHLAEKFGAFIPTAPAARAACLSWLFWQMGATPYLGGGF
                     GHFYAYANDKQQYPIDRFTMEVKRQLDLLDRQLAEHEYVAGSDYSIADTAIWPWYGAL
                     VVGEAYNAAEFLEAASYKNVLRWQQQIAARPAVQRGRLVNRRNGGLRERHAASDFDGV
                     WPKAAP"
     gene            4468..>4882
                     /locus_tag="PK242_11505"
     CDS             4468..>4882
                     /locus_tag="PK242_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOZ94687.1"
                     /translation="MSVLDHCHPDERAARLQLAACYRVFALLGWTEMIYNHITLRVPA
                     SATGGEKHFLINPFGLHYSEVTASNLVKINLAGDILSESAYPINPAGYVLHSCIHDGV
                     PGAHCVMHTHTTAGVAVASLASGLSQSNFYSAQLHD"
BASE COUNT          841 a         1740 c         1592 g          709 t
ORIGIN      
        1 ggcgcgtcat cggcaagcac cgcaagctga tgccgacggc gctggagcgc ctggtctggg
       61 gctttggcga cggctccacc ctgcaggcgg tggacacgcc ctggggcaag ctgggcgcgg
      121 tgatctgctg ggaaaactac atgccggcgc tgcgtctggc gatgtaccag cagcgcgtgg
      181 cgatctactg cgcgccaacc gccgacgacc gcgattcctg gcaatcgacc atgcagcaca
      241 tcgcgctgga agggcgctgc ttcgtgctgt cggcctgcca gcatctgcga cgcagggact
      301 ttccgggcga gcgcatgaac aaccgcctgc cggaggcacc cgacaccgtg ctgatgcgcg
      361 gcggcagcag catccacgac ccactgggcc agcggctggc cggcccggtg ttcggcgagg
      421 acgcgctgct caccgccgag ctggacctgt cgatcatccc cgccgcgcag atggatttcg
      481 acccggtcgg ccactatgcc cggccggacg tgttttcact tcatgtcgac acggcgccgc
      541 agcgcgccgt gcaactcgga gaccagtgat gggacagctc gcccttgccg ccaagatcac
      601 ccacgtgccc agcatgtacc tgagcgaact gcccggcgcg cgccagggca cgcgccagga
      661 cgccatcgac ggccacaagg aaatcggccg ccgctgccgc gagttggggg tggacacgat
      721 ggtggtgttc gacacccact ggctggtcaa cgccacctac cacctcaact gcgcgccgcg
      781 ctggcagggc agctacacca gcaacgagct gccgcacttc atcagcaaca tggcctacgg
      841 cttcaacggc aaccccgagc tggggcggct gctggccaag gcctgcaacg agcagggcgt
      901 ggagacgctg gcgcacgacg ccacttcgct gcagcccgag tacggcaccc tggtgccgat
      961 gcgctacatg aacgcggacg agcacttcaa ggtggtgtcg gtgtcggcgc tgtgcaccag
     1021 tcactattta aacgatagcg cgcgcctggg ctgggccatg cgcgaggcgg tggaaaagca
     1081 ctacgacggc aaggtcgcct tcatggccag cggctcgctg tcgcaccgct tcgcgcaaaa
     1141 cggcctggcg cccgaattcg ccttcaagat ctggagtccg ttgctggagc ggctggacca
     1201 cgaggtgatc cagatgtgga agaacggcga atggcccgac ttctgcgcca tgctgcccga
     1261 gtacgccacc aagggccacg gcgaaggcat gatgcacgac accgccatgc tgctgggcgc
     1321 tttgggctgg tcggcctacg acggcaagat ggaagtcatc acgccgtact tcgccgcctc
     1381 gggcaccggg cagatgaacg cggtgttccc ggtcaccccg caggacggcc acgccgtgcc
     1441 gccgcccgag gcctcgcgcg ccaccggttt tcgcgccttt gcgcgcatct gacgcaacgc
     1501 tgccatgccc catctggtca tcctttactc cggcaacctc gacgccatcg tcgacatgcc
     1561 cgccctgtgc cgcgacgctg ccgacgccat gctggcggtg cgcgacgaag cgggcgccca
     1621 ggtctttccc accggcggca cgcgcgtgtt ggcctatccg gcgccgcatt tcgcgctggc
     1681 cgatggcggc gcggcgggtc gtgcggccgg tgccggcaag acaggcatca cgggcagcga
     1741 tccgggcgaa tacggcttca tgtacgccaa cctgcgcatg gcgcgcgggc gcagtagcgc
     1801 cacgcagcag gccgccggcg aggcgattgc gcgggcgttg cgcgcgcggc tcgacccttg
     1861 catggccacg cgccacattg gcctgacggt gcaggtggac gaaggccccg aagtgttcga
     1921 cgccaaacac agcaccctgc acccgctgtt tcgcaaggcc tgacatgctg accgacgaac
     1981 agatccagca gctcgccgcc gaactgcacg ccagcgaaaa atcgctccag caggtcgagc
     2041 acttctccaa gcgctttccc ggcatgacga tcgaggacgg ctaccgcatc agccgcgcct
     2101 gggtggcgct gcagcgggcc gaggggcggc aggtgatcgg ccacaagatc ggcctgacct
     2161 cgcgcgccat gcagatctcc agccagatcg acgagcccga ctacggcacg ctgctggaca
     2221 acatgctcta cacctgcacg ccggggcagg tgctgccaat ccccaccgat cgcttcatcg
     2281 ccccgcgcgt cgaggtggag ctggccttcg tgctcaaggc cgacctcgct gggccgcacg
     2341 tcgatgtcga gcaggtgctg gccgcgaccg actacgtcac gcccgccatc gagatcatcg
     2401 acgcccgcat cgagcagttc gaccgccaca cgcgggtgat gcgcaaggtc tacgacacca
     2461 tcagcgacaa cgccgccaac gccggcatcg tggtgggcgc cggccgcgcc gacgcgcgcg
     2521 ccatcgatcg cgcctgggtc ggtgccatcc tgcgccagaa cggcgcggtc gaagaaaccg
     2581 gcctggccgc cggcgtgcag ggcgacccgg ccatcggcat cgcctggctg gccaacaagc
     2641 tcgcgccctg gggcgaggtg ctgcgcgcca ggcagatcgt gctggccggc tccttcaccc
     2701 gcccggtggc ggccaagcgc ggcgacgtgt tcgaggccga ctatgggccg ctgggtcaat
     2761 tgcagttcaa atttgtctga tcatggaaac accgctcaac cccttcaagc aggcgctgca
     2821 tgaaaagcgc cgccagatcg gcttctggct gaatctgccc aacaccacgg tggccgaaat
     2881 cgccgccggc gcgggctttg actggctgct gatcgacggc gagcacacct accacagcct
     2941 ggaaacgatc ctgcaccagc tgcagaccat cgccgcctat ccgcgcaccc acgccatcgc
     3001 gcgcctgccc atgggccacg gccatgtggg tgagatgctg atcaagcagt atctggacct
     3061 gggcgtgcag accctgctgg tgcccatggt ggacacgccc gagcaggcgg ccgccatcgt
     3121 gcgcgccgcg cgctatccgg gcgggggcgg cgcgggcggc atccgcggca tggcgggcgg
     3181 gcgcgccgcg cgctggggcc gctatccgaa gtacgtgcac caggccaatg accgcgtctg
     3241 catcctggtg caggccgaaa cgcgcaccgc gctggacaac ctggaagcga ttgccgcggt
     3301 ggacggtgtg gacggcatct tcatcggccc ggccgacctg tcggccagca tgggccaccg
     3361 cggcaacccg gcccaccccg agatgcaggc catcatggaa gacgccacgc gccgcatcgt
     3421 gcgcgccggc aaggcggccg gcaacctctc gcccgacgcc gccaccgccc agcgctacct
     3481 ggaatgggga gccaccttcg tcgccgtcgg gctggacacc aatgtgctgg cccgcgaaac
     3541 cagcgccctg gcgcagaagt tcgcggccgg gtgattcgcg ccggcctgtg gccgagctgt
     3601 cgtcaatgtg aaggagacgc gcatgagcag cgcatacgtt ccgcccaaag tctggcgctg
     3661 ggaaccgaaa gaagacaaca aattcgccaa catcaaccgg cccaccgccg gcgcgcgctt
     3721 cgagcaggag ctgccggtcg gccagcaccc gctgcagctg tactcgctgg ccacgcccaa
     3781 cggccagaag gtgaccatcc tgctggaaga gctgctggcc gccggccacg ccggcgccga
     3841 atacgacgcc tggctgatcg acatcggcga gggcgcgcag ttcggcagcg gctttgtggc
     3901 gatcaacccc aattccaaga tcccggcgct gctcgatcgc agcgtgcagc cggcgatccc
     3961 ggtgttcgaa tcgggcgcca tcctgctgca cctggccgaa aaattcggtg ccttcatccc
     4021 caccgcgccg gcggcgcgcg cggcctgcct gtcgtggctg ttctggcaga tgggcgccac
     4081 gccctacctg ggcggtggct ttgggcattt ctatgcctac gccaacgaca agcagcagta
     4141 ccccatcgac cgcttcacca tggaggtcaa gcgccagctc gacctgctgg accgccagct
     4201 ggccgagcac gaatacgtgg ccggcagtga ctacagcatc gccgacacgg ccatctggcc
     4261 ctggtacggt gcgctggtgg tgggcgaggc ctacaacgcc gcggagtttc tggaggcggc
     4321 cagctacaag aacgtgctgc gctggcaaca gcagatcgcc gcccgcccgg cggtgcagcg
     4381 cgggcgcctg gtcaaccgcc gcaatggcgg cctgcgcgag cgccatgcgg ccagcgactt
     4441 cgacggcgtt tggcccaagg ccgcaccatg agcgtgctgg accactgcca ccccgacgag
     4501 cgcgccgccc gtctgcaact ggccgcctgc tatcgcgtgt tcgccctgct cggctggacc
     4561 gagatgatct acaaccacat caccctgcgc gtgcccgcaa gcgccaccgg gggtgaaaag
     4621 cacttcctca tcaacccctt cggcctgcac tacagcgagg tcacggccag caacctggtc
     4681 aagatcaacc tggcgggcga catcctgagc gagtccgcct accccatcaa ccccgccggc
     4741 tatgtgctgc acagctgcat tcacgatggc gtgcccggcg cgcattgcgt catgcacacg
     4801 cacaccacgg ccggggtggc ggtggcctcg ctggccagcg ggctgtcgca aagcaacttc
     4861 tacagcgcgc aactgcacga ca
//
LOCUS       DAOOOG010000185         4845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_21066_length_4845_cov_27.494363, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000185 DAOOOG010000000
VERSION     DAOOOG010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21066_length_4845_cov_27.494363"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PK242_11510"
     CDS             complement(<1..517)
                     /locus_tag="PK242_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOZ94688.1"
                     /translation="MSAEATELLPKQELQAQVVQALQAVLPAHSLLWTDEQTTPYECD
                     GLTAYRQRPLAVALPDTAEQVREVLRVCHRLGVPVVARGAGTGLSGGALPHERGVTLS
                     LARFNRILAIDPLARTARVQCGVRNLAISEAAAAHGLYYAPDPSSQIACTIGGNVAEN
                     SGGVHCLKYGLT"
     gene            complement(821..1246)
                     /locus_tag="PK242_11515"
     CDS             complement(821..1246)
                     /locus_tag="PK242_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HOZ94689.1"
                     /translation="MDQPLRQPQDVPLRNVRSNIVQSIRAFHAHDLQQAAAQLGQHFL
                     YANLAHAQTKQDVLDTIAAQFTFPAHFGKNFDALYDCMTDPLHKSGAQPGFVVVLEHI
                     PATAKFDKEAREQLLDIFRDAADYWGDRKIPFRCFYSFL"
     gene            complement(1327..1662)
                     /locus_tag="PK242_11520"
     CDS             complement(1327..1662)
                     /locus_tag="PK242_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HOZ94690.1"
                     /translation="MAGVAQARGLSWGVEPATVAVAELPSQGQHTYRLILQGGPFPHD
                     KDGSVFGNRERLLPRERRGYYREYTVETPRARGRGARRIVCGGQRPTAPEACYYTSDH
                     YSSFRKIVQ"
     gene            complement(1882..4206)
                     /locus_tag="PK242_11525"
     CDS             complement(1882..4206)
                     /locus_tag="PK242_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOZ94691.1"
                     /translation="MTTTPPSQDDKREQLRQAALDYHEHPVPGKIAIAATKQLVNQYE
                     LSLAYSPGVAAPCEEIQRDPAAAFRYTARGNLVGVITNGTAVLGLGDIGALAGKPVME
                     GKAVLFKKFSGIDVFDIEINEKDPDKLVEIIAALEPTFGGINLEDIKAPECFTVEARL
                     RERLKIPVFHDDQHGTAIVVGAAVVNGLKVVGKKIEDVKLVVSGAGAAALACTGLLVD
                     LGVRRENVWVTDIEGLVYEGRTALMDEYKARYAQRTDKRTLAEVIEGADVFMGLSAGG
                     VLKPEMVARMAPRPLILALANPNPEILPEQVKAVRDDAVIATGRTDYPNQVNNVLCFP
                     YIFRGALDCGASTISKQMEVAAVYAIAGLAQAEQSEVVARAYAGEKLAFGPEYLIPKP
                     FDPRLMMKIAPAVAEAAAASGVATRPIVDLDAYRERLQTFVYASGTTMKPIFDAARAA
                     ASKRVAYAEGEEERVLRAAQIVVDEGLARPTLIGRPAIIAQRIVKFGLRLAEGADYDV
                     VNVENDVRYKGFWQEYYELTKRRGMTVPMAKIEMRRRLTLIGTMLLRAGQVDGLICGT
                     WGTIPMHLNYVDQVIGKRPGTHTYAAMNGVLLPGRQLFMVDTHINVDPTAEQLTEITI
                     AAAEQVQRFGLKPRAALLSHSNFGSANDASAVKMREVLERVHAQAPWLEIDGEMHGDV
                     ALDSGMRRALMPDSTLKGDANLLILPGIEAANIAYNLLKTAAGGNIAIGPVLLGAAKP
                     VHILTASATVRRIVNMTALAVADASAANRHQSNK"
     gene            complement(4382..4627)
                     /locus_tag="PK242_11530"
     CDS             complement(4382..4627)
                     /locus_tag="PK242_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HOZ94692.1"
                     /translation="MPRLIRTHLPTLKKTASYYVMHIAVAAAVAWLVTGDLLMALTLS
                     LLEPTVQAVAFFFHEKAWARVDGRSAAPAAAGPALGA"
BASE COUNT          741 a         1587 c         1669 g          848 t
ORIGIN      
        1 ccgtcaggcc gtatttcagg cagtgcacgc cgccggagtt ctcggccacg ttgccaccga
       61 tggtgcaggc gatctggctg gacgggtcgg gcgcgtagta caggccatgg gcggccgccg
      121 cctcgctgat ggccaggttg cgcaccccgc actgcacgcg cgccgtgcgc gccagcgggt
      181 cgatggccag gatgcggttg aagcgcgcca gcgacagcgt caccccccgc tcgtgcggca
      241 acgcgccgcc cgacagcccg gtgccggcgc cgcgcgccac caccggcaca cccaggcggt
      301 ggcacacgcg cagcacctcg cgcacctgct ccgccgtgtc gggcagcgcc accgccagcg
      361 gccgctggcg ataggcggtc aggccgtcgc actcgtaggg cgtggtctgc tcatccgtcc
      421 acagcagcga atgcgcaggc aaaacggctt gtagcgcttg taccacttgc gcctgcagct
      481 cttgttttgg tagcaattcg gtggcttcgg cggacatggt gtggctcctg gggcgatccc
      541 tgctgccgac tataggcgat gacccggaaa tcggcctgcc agccaatggc caacaaaaat
      601 gcccgcacac ggcgggcttg ggcgatcggc gatcggtttc aggcggcgct gagccagtag
      661 cccgcgttga acgggctgct catgcgcagc gccagcgggg aaatgtcgac cagcttgtcg
      721 gtcggcattt tttcgccatc gtcggcctcg atgccctggg gcgccggttc gccctgggcc
      781 tcgttcgccc gttctgcctg gccagtggtt gcagaacggg ctacagaaaa gaatagaagc
      841 acctgaacgg gatcttgcgg tcaccccagt agtcggcggc gtcgcggaag atgtcgagca
      901 gctgctcgcg cgcctccttg tcgaacttgg cggtggccgg gatgtgttcc agcaccacca
      961 cgaagccggg ctgcgcgccg ctcttgtgca gcgggtcggt catgcagtcg tacagggcgt
     1021 cgaagttctt gccgaagtgg gccgggaagg tgaactgcgc cgcgatcgtg tccagcacgt
     1081 cctgcttggt ctgggcatgg gccaggttgg cgtacaggaa atgctggccc agctgggccg
     1141 ccgcctgctg caggtcgtgc gcgtggaacg cgcggatcga ctgcacgatg ttgctgcgca
     1201 cattcctgag cggcacatcc tggggttgac gaagtggttg atccatcccc gctgctttct
     1261 ctgaagtcaa taaccggtcg gcgcccaccg ccacggcggg cgcgggctga aactgttcaa
     1321 acgccttcat tgcacgatct tcctgaagct cgaatagtga tcgctggtgt aataacaggc
     1381 ctcgggtgcg gtcgggcgct ggccgccgca gacgatgcgc cgtgcaccac ggccgcgcgc
     1441 gcgcggggtt tcgacggtgt attcgcggta gtagccgcgc cgctcgcgcg gcagcagacg
     1501 ctcccggttg ccgaacaccg acccgtcctt gtcgtgagga aatggcccgc cttgcagaat
     1561 cagccggtag gtgtgctggc cctgggacgg cagttcggcc accgccacgg tggccggctc
     1621 gaccccccag gacagcccgc gtgcctgggc cacgcccgcc atggggagtg cgaccgtcag
     1681 caccgaagct gccaaaacca accgggacac gcgggcagca ccggacgccg ctgttcgcca
     1741 catggaagac accctttcgg aaaccccggg ttaacccgaa aattccaagg cgctagtgtg
     1801 acgcatgcgt caccaaaaag caagcgcctg cctaaaaatc aggcagcgcc agcggggtga
     1861 aattcgtaag gtagcgtgta cttacttatt tgattgatgc ctgttggcgg cactggcgtc
     1921 ggccaccgcg agtgccgtca tgttgacgat gcggcgcacg gtggcgctgg ccgtgagaat
     1981 gtgcacaggc ttggcggcgc ccagcaacac cgggccgatg gcgatgttgc cgccggcggc
     2041 ggtcttgagc aggttgtagg cgatgttggc ggcctcgatg ccgggcagga tcagcaggtt
     2101 ggcgtcgccc ttgagcgtgc tgtcgggcat cagcgcgcgg cgcatgccgc tgtcgagcgc
     2161 cacgtcgccg tgcatctcgc cgtcgatttc cagccagggc gcctgggcgt gcacccgctc
     2221 cagcacttcg cgcatcttga ccgcgctggc gtcgttggca gaaccgaagt tggaatgcga
     2281 cagcagcgcc gcccgcggct tgaggccaaa gcgctgcacc tgttcggccg ccgcgatggt
     2341 gatctcggtc agctgctcgg cggtcggatc gacgttgatg tgggtgtcga ccatgaacag
     2401 ctgcctgccg ggcagcagca cgccgttcat ggcggcatag gtgtgggtgc cggggcgctt
     2461 gccgatcacc tggtccacat agttcaggtg catggggatg gtgccccagg tgccgcagat
     2521 caggccgtcc acctggccgg cgcgcagcag catggtgccg atcagggtca gacggcggcg
     2581 catctcgatc ttggccatcg gcaccgtcat gccgcggcgc ttggtcagtt cgtagtattc
     2641 ctgccagaag cccttgtagc gcacgtcgtt ctcgacgttg acgacgtcgt aatcggcgcc
     2701 ttcggccagg cgcaggccga atttgacgat gcgctgggcg atgatggcgg gacggccgat
     2761 cagggtgggc cgtgccagcc cctcgtcaac cacgatctgc gccgcgcgca gcacgcgctc
     2821 ttcctcgccc tcggcgtagg ccacacgctt gctggcggcg gcgcgggcgg cgtcgaagat
     2881 cggcttcatc gtcgtgcccg aggcgtagac gaaggtctgc aggcgttcgc ggtaggcgtc
     2941 cagatcgacg atcggccgcg tggcgacgcc gctggccgcc gccgcttcgg ccaccgccgg
     3001 cgcgatcttc atcatcaggc gcgggtcgaa cggcttgggg atcaggtatt cggggccgaa
     3061 ggccagcttt tcaccggcgt aggcacgcgc caccacttcg ctctgctcgg cctgcgccag
     3121 gccggcgatg gcgtacacgg ccgcgacctc catctgcttg ctgatggtgc tggcaccgca
     3181 atcgagggcg ccgcggaaga tgtaggggaa gcacaggacg ttgttgacct ggttggggta
     3241 gtcggtgcgg ccggtggcga tgacggcgtc gtcgcgcacc gccttgacct gctcgggcag
     3301 gatctcgggg ttggggttgg ccagcgccag aatcagcggg cgcggcgcca tgcgcgccac
     3361 catctcgggc ttgagcacgc cgccggccga caggcccatg aacacgtcgg cgccctcgat
     3421 cacctcggcc agggtccgct tgtcggtgcg ctgggcgtag cgcgccttgt attcgtccat
     3481 cagcgccgtg cggccctcgt acaccaggcc ctcgatgtcg gtgacccaga cgttttcgcg
     3541 ccgcacgccc aggtccacca gcaggccggt gcaggccagc gccgccgcac cggcgccgga
     3601 caccaccagc ttgacgtctt cgattttctt gcccaccacc ttcaggccgt tgaccaccgc
     3661 ggcgccgacc acgatggcgg tgccatgctg gtcgtcatgg aacaccggga tcttcagccg
     3721 ctcacgcagc ctggcttcga cggtgaagca ttcgggcgcc ttgatgtctt ccaggttgat
     3781 gccgccgaag gtcggctcca gcgcggcgat gatctcgacc agcttgtccg ggtccttctc
     3841 gttgatctcg atgtcgaaca cgtcgatgcc cgagaacttc ttgaacagca ccgccttgcc
     3901 ttccatcacc ggcttgccgg ccagcgcacc gatgtcgccc aggcccagca cggcggtgcc
     3961 gttggtgatc acgcccacca ggttgccgcg tgcggtgtac ctgaaggcgg ccgccggatc
     4021 gcgctggatc tcctcgcagg gcgcggcaac gccggggctg taggccagcg acagctcgta
     4081 ctggttgacc agctgcttgg tggccgcgat ggcgatcttg ccgggcaccg ggtgctcgtg
     4141 atagtccagc gccgcctggc gcagctgctc gcgtttgtcg tcttggctcg gtggcgtggt
     4201 ggtcatggtc ggcgtcctgc agtggctggc cctgccggtg gcgcaccacg gcaggcggcc
     4261 ggtcattgta ggcagcggcg ggcgtgcagc cagttcacct ggcaggcgac ggccgtcgaa
     4321 accaccagac ccgcaggggt gcggcccgcc cgcagccggc gggcggcctt gagcgccgcg
     4381 ttcaggctcc gagcgccggc ccggccgccg ccggcgcggc gctgcggccg tccacgcgcg
     4441 cccaggcctt ttcatggaag aagaaggcca ccgcctgcac ggtgggctcc agcaggctga
     4501 gggtgagcgc catcagcagg tcacccgtca ccagccaggc cacggcggca gccaccgcga
     4561 tgtgcatcac gtagtagctg gcggtcttct tgagcgtggg caggtgggta cggatcaggc
     4621 gtggcatggg gactcccggc agttcattca atgataatga ttctcattac gaatggcaat
     4681 cgatgcggcc tatgcgcccg atagccggcg cccacagggt gtggcacgga tgacggcagg
     4741 ggcgctaaca tgcgcgcttc gttgactttc tgttgcagga gctccggcca tggcactgat
     4801 ggatttcatc aagaagcagt tcatcgacat cctggagtgg acgga
//
LOCUS       DAOOOG010000186         4813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_21235_length_4813_cov_23.278058, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000186 DAOOOG010000000
VERSION     DAOOOG010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21235_length_4813_cov_23.278058"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..590
                     /locus_tag="PK242_11535"
     CDS             171..590
                     /locus_tag="PK242_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOZ94693.1"
                     /translation="MSTVKPVSQAETTPRVKAVFDDIRATRGSEHINHFWRYLAFDPS
                     LLEATWAEVKQVMATPSALDPLTKELIYIAVSVANGCGYCVHSHTAAARAKGMSAAQH
                     ADLLAVVGLAARTNHLATALQVPVDPAFDRDAVPDRS"
     gene            732..3320
                     /locus_tag="PK242_11540"
     CDS             732..3320
                     /locus_tag="PK242_11540"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOZ94694.1"
                     /translation="MQPVSTQSLLIKRARQEVVFLAGPLIDRCVEASISGLQEAERRG
                     RTTAIRMQLGDAWLALTRRRAELRDAFPPRVEQAMNQALEVARAAPSHSARAPLAHDE
                     LTLLEHAEVARFVEASRLQQSVMPLVEAPLARLDSLMSTALGLPVVRADLNPLRPDVL
                     CQALLALFDEQPVEAEWRVLWVRHLAPSFAQELSQLYEAVTRLLVDQGVEEARYRLKL
                     NEDGASTGASVPVPASTKAQPSGGGGGAGGFAGGAAPGSADGGGARRRPIARMADLAQ
                     AEPAVSRTLMRDFLYRGQWTGEHDEPLPAAYYEAVGAQQQRLAAAPAAAYDALAAAQA
                     RALAQAQSVVDRPARRVEIGQSLAPEQWGELGSAQARTRTLMALKSRATWISQVLGLD
                     AVRALVGQVADDERVLAPVREAFVAMEPALLRLAMRDPRFLGDEQHPARRLVEAVAQR
                     SFRHNDEFADEFEQFMAPVRQAVRELDALPQARPQDFERHLQALEAGWQKQDEGEAEA
                     PEHGLRSIQFAQERQALADKVAWEFSLRSDLDGVPAVVADFLFQDWSLVIAHAQLTDT
                     RGQLDPGGYLAVVSDLLWSVKREATLKDPARAFEVLPEVVRTLRRGLDMLGKDPAEAQ
                     TFFDVLMRCHDPVLRLRRMRSARDAQASGQGPLTVISAAAPLDIVALPLERPTPRAAE
                     QPWLGRRELALAGFDHEAGDSGADSLHSSLNALSRPIESPSPDGAGHQADAAASASAP
                     LPADLPRPQPSDSLPLSEPAGAAADAGESAADASERARAQLAHLRTGDWVDLKVREQW
                     RRARLHWCSDNGALFMFISRGGQPHSMTRRSCEKLLRARKLRPVESGAVVERALSELP
                     DAQPVT"
     gene            complement(3442..4623)
                     /locus_tag="PK242_11545"
     CDS             complement(3442..4623)
                     /locus_tag="PK242_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOZ94695.1"
                     /translation="MATGRARKVLIALALLALLGLAALLGLPGFVDARMNAIALAPPY
                     PVSTRAQQLHRRLFVADLHDDLLLWPRDPLQRHGHGQTDVPRLREGGVSLQVFTTVTK
                     TPRGMNFERNAGDSDNITLLAMAQRWPPRTWTSLLERALHQSRKLHQAARDSHGQLRL
                     VTSQAQLAAALADGLPAARGQLLAVLGTEGLHPLEGRLDHIDRLYEAGFRVMGLTHFF
                     DNEVGGSAHGLDKGGLTPFGHEVVRQLQQRRMIVDLAHASPAVIDDVLAVTRRPVIVS
                     HTGVQAVCPGPRNLSDAQIRAIAARGGVMGMGFFQGAMCELSVERIVRSIRHVAQVAG
                     VQHVALGSDFDGATRTVFDGTGLALITEGLLQAGFSEAEIADIMGGNVLRLLQTELPA
                     N"
     gene            complement(4629..>4813)
                     /locus_tag="PK242_11550"
     CDS             complement(4629..>4813)
                     /locus_tag="PK242_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOZ94696.1"
                     /translation="RRSPTDDPAGPASGKVRVRRGGSWHTWPLYARVAFRNWNTPETR
                     YVLLGFRLLREQGPAR"
BASE COUNT          689 a         1634 c         1785 g          705 t
ORIGIN      
        1 gttcgcgagg aaggggccga gatgcgggaa aaaatcagtt cggggtggtg ccccagccaa
       61 gcggccggtg tgctgctgct cgtatggcca ccggggcgtg tgacggcgct gggtgggttg
      121 ggggggcagc gcgtcgccgg cagggtcacg acgccggggg gctgagtacc atgtccaccg
      181 taaagcccgt gagccaggcc gagaccaccc cgcgggtcaa ggccgtgttc gatgacatcc
      241 gggccacgcg cggttcggaa cacatcaatc atttctggcg ctacctggcc tttgacccct
      301 cgctgctcga agcgacctgg gccgaggtca agcaggtcat ggccacgcct tcggcgctgg
      361 atccgctgac caaggaactg atctacatcg ccgtgtcggt ggccaatggc tgcggctact
      421 gcgtgcattc gcacaccgcg gcggcacggg ccaagggcat gtcggcggcc cagcacgccg
      481 atctgctggc ggtggtgggg ctggccgcca ggaccaatca tctggcgacg gcgctgcagg
      541 tgccggtgga cccggccttc gaccgcgacg cagtgcctga ccgatcctga cacccacggt
      601 gcgggggcgc caccggaccg gtgtggcgtg gagtgctgcc tgaactccaa atgcttatat
      661 gagccagcgc aaattgtgtg cacccatggc tcgcccacgg gaaaatttgc gtaagatagt
      721 tctattgcgc catgcaaccc gtcagcaccc agagccttct gatcaagcgc gcgcggcagg
      781 aagtcgtgtt cctggccggg ccgctgatcg accgctgtgt cgaggccagc atcagcggtc
      841 tgcaggaagc cgagcgtcgc ggtcgcacca cggccatccg catgcagctg ggcgacgcct
      901 ggctggcgct gacccgccgc cgcgccgagt tgcgagacgc gtttccgcct cgcgtggagc
      961 aggccatgaa ccaggcgctg gaggttgcgc gcgctgcacc ctcccactcg gcgcgtgcgc
     1021 cgcttgcgca cgacgagttg accctgctcg agcacgccga ggtggcgcgt tttgtcgagg
     1081 catcgcgact gcagcagagc gtgatgccgt tggtcgaggc gccgctggcg cggctggatt
     1141 cgctgatgag cacggccctg ggcctgccgg tggtgcgggc cgatctgaac ccgctgcgtc
     1201 ccgacgtgct gtgtcaggcg ctgctggccc tgttcgacga gcagccggtg gaggccgagt
     1261 ggcgcgtgct gtgggtacgc catctggcgc cgtcttttgc ccaggagctg agccagctgt
     1321 atgaggcggt gacccgcctg ctggtggatc agggcgtgga agaagcgcgc taccgcctga
     1381 agctgaatga agacggggcg tcgaccgggg cgtcggtgcc ggtgccggca tccacgaagg
     1441 cgcaaccctc cggcggtggt ggcggcgccg gtggctttgc cggtggggct gcgccggggt
     1501 cggccgatgg tgggggagcg cgccgccggc cgattgcgcg catggccgat ctggcccagg
     1561 ccgagccggc ggtatcgcgc acgctgatgc gcgactttct gtaccggggg cagtggacgg
     1621 gcgagcacga tgagcccttg ccggccgcct actatgaagc ggtgggggcg cagcagcagc
     1681 gcctggccgc cgcgccggca gcggcctacg acgcgctggc ggcggcccag gcgcgcgcgt
     1741 tggcccaggc tcagagcgtg gtcgaccggc cggcgcgccg ggtcgagatc gggcagtcgc
     1801 tggcacccga gcagtggggt gaactgggct cggcccaggc gcgcacgcgt accctgatgg
     1861 cgctcaagtc ccgcgccacc tggatcagcc aggtgctcgg gctggacgcg gtgcgcgctt
     1921 tggtgggcca ggtggccgac gacgaacgcg tgctggcacc ggtgcgcgag gccttcgtgg
     1981 ccatggagcc ggcgctgctg cggctggcca tgcgcgaccc gcgtttcttg ggcgatgagc
     2041 agcacccggc caggcgtctg gtcgaggcgg tggcccagcg cagcttccgc cacaacgacg
     2101 agtttgccga cgagttcgag cagttcatgg cgccggtacg tcaggcggtg cgcgagctcg
     2161 acgccctgcc gcaggccagg ccacaggact tcgagcgcca cctgcaggcg ttggaggcag
     2221 gctggcagaa gcaggacgag ggcgaagccg aggcgcctga gcatggcctg cgctcgatcc
     2281 agttcgccca ggaacgccag gcgctggccg acaaggtggc ctgggaattc agcctgcgct
     2341 ccgacctcga cggcgtgccg gcggtggtgg ccgattttct gtttcaggac tggtcgctgg
     2401 tcattgccca tgcccaactg accgacacgc gcggccagct cgaccccggg ggctacctgg
     2461 cggtggtgag cgatctgctc tggagcgtga agcgcgaggc cacgctcaag gacccggcac
     2521 gcgccttcga ggttctgccc gaagtggtgc gcaccttgcg ccgtggcctg gacatgctgg
     2581 gcaaggatcc ggcagaggca cagaccttct tcgacgtgct gatgcgctgc cacgacccgg
     2641 tgctgcggct gcgccgcatg cgcagcgccc gcgatgccca ggcctcgggg cagggcccgt
     2701 tgacggtgat ctcggccgcc gcgcccctgg acatcgtggc gctgccgctg gaacggccca
     2761 cgccgcgcgc tgccgagcag ccctggctgg gccggcgtga gctggcgctg gcgggcttcg
     2821 accacgaagc gggcgacagc ggtgcggaca gcctgcacag cagcctcaat gcgttgtcgc
     2881 gcccgatcga gtcgccgtcg cccgacggcg cgggccacca ggcggacgcc gcggcgtccg
     2941 catcggcgcc gctgcctgcc gatctgccgc ggcctcagcc ctcagattcg ctgccgctgt
     3001 ccgagcccgc cggcgcggcg gccgacgctg gcgagagcgc cgccgacgcc agcgagcgcg
     3061 cccgtgccca gctggcgcac ctgcgcaccg gcgactgggt cgacctcaag gtgcgcgagc
     3121 agtggcgacg cgcgcgactg cactggtgca gcgacaacgg cgccctgttc atgttcatca
     3181 gtcgcggcgg ccagccgcac agcatgacgc ggcgcagctg cgaaaaactg ctgcgcgcgc
     3241 gcaagctgcg cccggtggaa tccggcgccg tggtcgagcg cgccctgagc gaactgccgg
     3301 acgcccagcc ggtcacctga acaggcggcc agacgctggc cgcggctgag acggcttggc
     3361 gtcagaccct gggtttcaga tcaaataggc ttgtggcgcg ccatgggtaa gcgccaccag
     3421 ctatcattag agtagcaagc gtcagtttgc cggcagctcg gtctgcagca gccgcagcac
     3481 gttgccgccc atgatgtcgg cgatttcggc ttcgctgaag ccggcctgca gcaggccctc
     3541 ggtgatcagc gccaggccgg tgccgtcgaa caccgtccgg gtggcgccgt cgaagtccga
     3601 gcccagggcg acgtgctgca ccccagccac ctgggccacg tggcggatcg aacggacgat
     3661 gcgctcgacg ctgagctcgc acatggcgcc ctggaagaag cccatgccca tcacgccgcc
     3721 gcgggcggca atggcgcgga tctgggcgtc gctcaggttg cgtgggcccg ggcagaccgc
     3781 ctgcacgccg gtgtgcgaga cgatcaccgg ccgccgcgtg acggccagca cgtcgtcgat
     3841 cacggcgggc gaggcgtgcg ccagatcgac gatcatgcgc cgctgctgca actggcgcac
     3901 cacctcgtgg ccgaacggcg tcaggccgcc cttgtccagg ccgtgggccg agccgccgac
     3961 ctcgttgtcg aagaaatgcg tcagacccat gacgcgaaag ccggcctcgt acagacggtc
     4021 gatgtggtcc agccggcctt ccagcgggtg cagcccttcg gtgccgagca cggccagcag
     4081 ctgaccgcgc gcggccggca ggccgtcggc cagcgccgcc gccagctgcg cctgcgaggt
     4141 caccagccgc aactgaccgt ggctgtcgcg cgccgcctgg tgcagtttgc ggctctgatg
     4201 gagcgcgcgc tcgagcaggc tggtccaggt gcgcggcggc cagcgctggg ccatcgccag
     4261 cagggtgatg ttgtcgctgt cgccggcgtt gcgctcgaag ttcatgccgc gcggcgtctt
     4321 ggtcacggtg gtgaacacct gcaggctgac cccgccttcc cgcaggcgcg gcacgtcggt
     4381 ctggccgtgg ccgtggcgct gcagcgggtc gcgcggccac agcagcaggt cgtcgtgcag
     4441 atcggccacg aacagccggc ggtgcagctg ttgcgcgcgt gtgctgacgg ggtagggcgg
     4501 cgccagggcg atggcgttca tgcgcgcatc cacgaagccc ggcaggccaa gcagcgccgc
     4561 cagtccgagc agcgccagca gcgccagcgc gatcaggacc ttgcgcgcgc gcccggtggc
     4621 cacggcgttc agcgcgccgg cccctgctcg cgcagcaggc gaaagcccag cagcacgtag
     4681 cgcgtttccg gcgtgttcca gttgcggaat gccacccgcg catacagcgg ccaggtgtgc
     4741 caggagccgc cgcggcgtac ccgcaccttg ccgctggccg ggccggcggg gtcgtcggtg
     4801 ggcgagcggc ggt
//
LOCUS       DAOOOG010000187         4807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_21275_length_4807_cov_22.892887, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000187 DAOOOG010000000
VERSION     DAOOOG010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21275_length_4807_cov_22.892887"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..4483
                     /locus_tag="PK242_11555"
     CDS             245..4483
                     /locus_tag="PK242_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94697.1"
                     /translation="MQGQLFSQDFLLRGVLDTPPWQALDDAAFAPFEGAVRALFAALP
                     AGAPINEAQTESLLIVPLLRALGWGDELLPQVNADARGRESVPDWLLFASAEHKAAAL
                     AEPRDDRRYRHGVAILEAKRWQRPLDRGDAAEVFDPDAPSSQMLRYLSRVDVASDRAL
                     KWGVLTNGAVWRLYWQDARSRSEDFFELDLAQALALPGTQPELDAPAPAHALRLFHLF
                     FSRDAFLPQPWDAAGRTRHQHALNEARLYEEKVSQDLGARVFNEVFPQLADALTRDDL
                     AARRHTIGYGSYTRPRFDDDYLDEVREATLVLLYRLLFLFYAEDRRLLPVHDERYAEY
                     SLRRIREQVRDKVDAGGAFSSTMPKIWLALQGVFRLIDEGDDDIGMPAYNGGLFSRAR
                     APLLERTTVPDKLLAPLIDALSRRTEDLLKGWINYRDLSVAHLGGIYERLLEYKLVHE
                     VQARHSYRELPEIDRIVARPASFARKVSGSYYTHADLVRLLLRESVGRLAAERMAAFE
                     QALAGWQARATLGPAQWERLDGSTAQAQRDAIDPATQILELTVCDPAMGSGHFLVALV
                     DDLADRVLEAASRASALVNAQPWAAHLAERGHAWESPVVGRIAAIRREIKAQAARHGW
                     AVTDAQLDDRHIVRRMILKKCVFGVDKNAMAVELAKTALWLHTFTVGAPLSFLDHHLK
                     CGDSLHGEPLAPVRRGLQALGTLFDTGALERLALAAQGLAQVADLTDVDIAEARQSQQ
                     LAQEAEARTAPLHRLLDFWRALRWLLPGWPVDKPAKLQKLGDDATRGALAALLAPGRN
                     LVETLSAGALDASEPGAADAAALMQACAQLAARERFFHWWTAFPTVFDLEGRGGFDVV
                     LGNPPWDRIKLQEVEWFAERDAAIASQPRAADRKALIAALQHKNAPLAAEYAQAAERA
                     EANARVLAKAGDYPLLGGGDVNLYSLFVERAQALVQHGGLVALLTPSGIAADKGAAEF
                     FRGISAGGRLGALFDFENRKGLFPDVDSRFKFCVLLFGGPERRFEQTRCAFFLHSLDE
                     LDTQPERTLVLSADDFARVNPNTGAAPIFRSRRDADITLGIYARHPVLVRHGPQGEQK
                     AWPVKYLRMFDMTNDSGKFMKPEELERGGWQRAPLGRWRRGKEDALPLYVGRMIHQYD
                     HRSASVDLNEDNLKVATLSDRAASHQKAAADFHPVPQYWVRASDVPVQAQREWALGFR
                     DIARATDVRTMIAAIVPGTVAGNTLPLLVDEAMSGRNAALLLSNFNAMAFDYIARQKA
                     QTTHLNWYIVEQLPVITPTAFDQPLPAPFAAAARAAGLMNGHHPDPTVADFVLPQVLA
                     LSYTAHDLAPFARDLGYVDAHGQPLPPIVWNDDERRVRLAALDALFFWLYGLDADDAA
                     YVLGTFPIVREQDERAFGRFRTREDVLRWRALLA"
     gene            4650..>4807
                     /locus_tag="PK242_11560"
     CDS             4650..>4807
                     /locus_tag="PK242_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HOZ94698.1"
                     /translation="MTTSTSPPPRSKWSLYLDLIRWDRPAGWLVLLWPTLSALWLAAG
                     GFPGWHLLA"
BASE COUNT          700 a         1786 c         1614 g          707 t
ORIGIN      
        1 agcttcaccc gctacgaagc catcgccgcc ctgcgcaacg ccatctacgc ctgcgaactg
       61 ccgggggtgc agcggctgtc gtgagccgtc agggcgcgcc cggcacaagc aataaaatga
      121 acctcagggg cttaccagat aagcgccatt agctacaaca attgtagctt tcgggtgctg
      181 ggccgagctg cacttcgacg cttacacttg cagcccatcc ggcgcccgca tccactgccc
      241 attcatgcaa ggtcagctct tttcccagga cttcctgctg cgcggcgtgc tcgacacgcc
      301 accctggcag gcgctcgatg acgcggcctt cgcgccgttc gagggtgccg tgcgcgccct
      361 gtttgccgcg ctgccggccg gcgcgccgat caacgaggcg cagaccgagt cgctgctgat
      421 cgtgccgctg ctgcgcgccc tcggctgggg cgacgagttg ctgccgcagg tcaacgccga
      481 cgcgcgcggg cgcgagtcgg tgcccgactg gctgctgttt gccagcgccg agcacaaggc
      541 cgccgcgctg gccgagccgc gcgacgaccg ccgttaccgc cacggcgtcg ccattctgga
      601 ggccaagcgc tggcagcgcc cgctcgaccg cggcgacgcc gccgaggtgt tcgaccccga
      661 cgccccctcg tcgcagatgc tgcgctacct cagccgggtc gacgtcgcct ccgaccgcgc
      721 gctcaagtgg ggcgtgctga ccaacggtgc ggtctggcgc ctgtactggc aggacgcgcg
      781 ctcgcgctcg gaagactttt tcgagctcga cctggcgcag gccctcgccc tgcccggcac
      841 ccagcccgag ctggacgcac ccgcgccggc gcacgcgctg cggctgtttc acctgttctt
      901 cagccgcgac gcctttttgc cccagccctg ggacgctgcc ggccgcacgc gccaccagca
      961 tgcgctgaac gaggcgcggc tgtacgaaga aaaggtctcg caggacctgg gcgcgcgcgt
     1021 cttcaatgaa gtcttcccgc aactggccga cgccctcacg cgcgatgacc tggccgcgcg
     1081 ccgccacacc atcggctacg gcagctacac gcgcccgcgg ttcgacgacg actacctcga
     1141 cgaagtgcgc gaagccaccc tggtgctgct gtaccgcctg ctgtttctgt tctacgccga
     1201 agaccgacgc ctgctgccgg tgcacgacga gcgctacgcc gaatacagcc tgcgccgcat
     1261 tcgcgagcag gtgcgcgaca aggtcgacgc cggcggcgcc ttctccagca ccatgcccaa
     1321 gatctggctg gcgctccaag gggtgtttcg cctgatcgac gagggcgacg acgacatcgg
     1381 catgccggcc tacaacggcg gcctgttcag ccgcgcgcgc gccccactgc tggagcgcac
     1441 caccgtgccc gacaagctgc tggcgccgct gatcgacgcg ctgtcgcgtc gcaccgagga
     1501 cttgctcaag ggttggatca actaccgcga cctgtcggtg gcgcacctgg gcggcatcta
     1561 cgagcgcctg ctcgaataca agctggtgca cgaggtgcag gcgcgccaca gctaccgcga
     1621 actgcccgag atcgaccgca tcgtcgcgcg cccggccagc tttgcgcgca aggtctcggg
     1681 cagctactac acgcacgccg acctggtgcg cctgctgctg cgcgaatcgg tcggccgcct
     1741 ggccgccgag cgcatggccg cgttcgagca ggcgctggcc ggctggcagg cgcgcgccac
     1801 cctcggcccg gcgcagtggg agcgcctgga cggcagcacc gcgcaggcgc agcgcgacgc
     1861 catcgacccg gccacgcaaa tcctggaact gacggtgtgc gacccggcca tgggcagcgg
     1921 ccacttcctg gtcgcgctgg tcgacgacct ggccgaccgc gtgctggagg ccgccagccg
     1981 cgccagcgcc ctggtcaacg cccagccctg ggcggcgcac ctggccgagc gcggccacgc
     2041 ctgggaaagc ccggtggtcg ggcgcatcgc cgccatccgg cgcgaaatca aggcgcaggc
     2101 cgcgcgccac ggctgggccg tgaccgacgc ccagctcgac gaccgccaca tcgtgcgccg
     2161 catgatcctg aagaaatgcg tgttcggcgt cgacaagaac gccatggcgg tcgagctggc
     2221 caagaccgcg ctgtggctgc acaccttcac cgtcggcgcg ccgctgtcgt ttctggacca
     2281 ccacctcaag tgcggcgact cgctgcacgg cgagccgctg gcgccggtgc gccgcggcct
     2341 gcaggcgctg ggcacgctgt tcgacaccgg cgcgctggag cggctggcgc tggccgccca
     2401 ggggctggcg caggtggccg acctgaccga cgtcgacatc gccgaggcgc gccagtcgca
     2461 gcagctcgcg caggaggccg aggcgcgcac cgcgccgctg caccgcctgc tcgacttctg
     2521 gcgcgcgctg cgctggctgc tgcccggctg gccggtcgac aagcccgcca aactgcaaaa
     2581 gctgggcgac gacgccaccc gcggcgccct ggcggcgctg ctggcgccgg ggcgcaatct
     2641 ggtcgaaacg ctgtccgccg gtgcgctgga cgccagcgaa cccggcgccg cagacgccgc
     2701 cgcgctgatg caggcctgcg cccagctggc cgcgcgcgag cgcttcttcc actggtggac
     2761 cgcctttccc accgtgttcg acctggaggg gcgcggcggc ttcgacgtgg tgctgggcaa
     2821 cccgccgtgg gaccgcatca agctgcagga ggtcgagtgg tttgccgagc gcgacgccgc
     2881 catcgccagc cagccgcgcg ccgccgaccg caaggccctg atcgccgcgc tgcaacacaa
     2941 gaacgccccg ctggcggccg aatacgcgca ggccgccgag cgcgccgagg ccaacgcccg
     3001 cgtgctggcc aaggccggcg actacccgct gctgggcggc ggcgacgtca acctctacag
     3061 cctgttcgtc gagcgtgcgc aggcgctggt gcagcacggc gggctggtgg ccctgctcac
     3121 ccccagcggc atcgccgccg acaagggcgc ggccgagttc tttcgcggca tcagcgccgg
     3181 cgggcggctg ggcgcgctgt tcgacttcga gaaccgcaag ggcttgtttc cggacgtgga
     3241 ctcgcgcttc aagttctgcg tgctgctgtt cggcggcccc gagcggcgct tcgagcagac
     3301 gcgctgcgcc ttctttctgc acagcctgga cgagctggac acccagcccg agcgcaccct
     3361 ggtgctgagc gcggacgact ttgcccgcgt caaccccaac accggcgccg ccccgatctt
     3421 tcgcagccgg cgcgacgccg acatcaccct gggcatctac gcccgccacc cggtgctggt
     3481 gcgccacggc ccgcagggcg agcagaaggc ctggccggtg aagtacctgc gcatgttcga
     3541 catgaccaac gactcgggca agttcatgaa acccgaggag ctggagcgcg gcggctggca
     3601 gcgcgcgccg ctgggccgct ggcgcagagg caaggaggac gcgctgccgc tgtacgtggg
     3661 gcgcatgatc caccagtacg accaccgctc ggccagcgtc gatctgaatg aggacaacct
     3721 gaaggtcgcc accctgtccg atcgcgccgc cagccaccag aaggccgccg ccgactttca
     3781 tcctgtgccg cagtactggg tgcgcgccag cgacgtgccg gttcaggccc agcgcgaatg
     3841 ggcgctgggg tttcgtgaca tcgcccgcgc caccgatgtg cgcaccatga ttgcggcgat
     3901 cgtgccgggt acggttgcgg gcaacacctt gccgctgctg gtggacgagg ccatgagcgg
     3961 gcgtaacgcc gcactgctgt tgtccaactt caacgcgatg gcgttcgatt acatcgctcg
     4021 acagaaggcg cagaccacgc acctcaattg gtacatcgtt gaacaactcc ccgtcatcac
     4081 ccccaccgcc ttcgaccagc ccctgcccgc gccctttgcc gccgccgcgc gcgcggccgg
     4141 cctgatgaac ggtcaccacc ccgacccgac ggtcgccgac ttcgtgctgc cgcaggtgct
     4201 ggcgctcagt tacaccgcgc acgacctggc gccgttcgcg cgcgacctgg gctacgtgga
     4261 cgcgcacggc cagccgctgc cgccgatcgt ctggaacgac gacgagcgcc gcgtgcgcct
     4321 ggcggcgctg gacgcgctgt tcttctggct ctacggcctg gacgccgacg acgcggccta
     4381 tgtgctgggc accttcccga tcgtgcgcga gcaggacgag cgcgccttcg gccgctttcg
     4441 cacgcgcgag gacgtgctgc gctggcgcgc gctgctggct tgaggcgcct cgtcgccgcg
     4501 ccggccttgc cccctctgcc gctgggagag cgctggggtg agggctgggg tgagggctgg
     4561 tggtggcccc attccacgcc gaccaaattg aagcaaaatc agcctttggc gcttacccac
     4621 caagcgccag cagctattga tttgatagta tgaccacatc gacatccccc ccgccccgca
     4681 gcaagtggtc gctctacctc gacctgatcc gctgggatcg cccggccggc tggctggtgc
     4741 tgctgtggcc caccctgtcg gcgctgtggc tggcggccgg cggctttccg ggctggcacc
     4801 tgctggc
//
LOCUS       DAOOOG010000188         4806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_21284_length_4806_cov_31.348348, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000188 DAOOOG010000000
VERSION     DAOOOG010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21284_length_4806_cov_31.348348"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PK242_11565"
     CDS             complement(<1..187)
                     /locus_tag="PK242_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOZ94699.1"
                     /translation="MNSLLLAFGALDARLALDCRLLSLRRHELPVRIGVHEFERQGPQ
                     RLWFDVDLCVRLRDAAAA"
     gene            complement(184..996)
                     /locus_tag="PK242_11570"
     CDS             complement(184..996)
                     /locus_tag="PK242_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ94700.1"
                     /translation="MSAPTAPLPVRAVLVTGAARRLGREISLQFARAGWDVLAHYRHA
                     EAEAQALQADVERLGRRCHLLRADLAQPEAACGLLRQASTLHGAPPHCIVNNASIFEA
                     DDARSASAAGLIQHFATNCATPLLLANALASLLAAGGQPSGGRHSVVHVLDQKVHNLN
                     PDYFSYTVSKLALAQSVALQAQALAPWLRVCGLSPGLMYLSGPQTADNFARASRVNLL
                     RQPIDAAEVARCALFIADNMSLNGCTLRADNGQHLVPLGRDVMFAVDEGLTR"
     gene            1060..2250
                     /locus_tag="PK242_11575"
     CDS             1060..2250
                     /locus_tag="PK242_11575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOZ94701.1"
                     /translation="MPTVPADATATASPAPDALPALIAREIRAAGGWLPFERFMHLAL
                     YAPGLGYYARAARQFGQLPRSGSDFVTAPELSPLFARALAVQLADSLAATGTDEVWEF
                     GAGSGALAAGLIEALDALGQPLARYTIVDVSGALRARQRQRLAAHGARVAWADALPPV
                     LRGVVVGNEVLDAMPVTLLARRGGRWFERGVRIGESIEALAQDGQAPAAIKTEAIGLT
                     WEDRPTALRPPLEPAGTHDLVTEIHPQAQAFVATVAEALRRGGAGAAWFIDYGFPEAE
                     YYHPQRSEGTLVCHRAHRVDDQPLADVGDKDISAHVNFTGVALAAQDAGLEVLGYTSQ
                     GRFLLNCGLAEALAAADLPTRANAMKLLDEHEMGELFKVIGLATPGHPPARGFAQGDR
                     THRL"
     gene            2256..2450
                     /locus_tag="PK242_11580"
     CDS             2256..2450
                     /locus_tag="PK242_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOZ94702.1"
                     /translation="MIRWLIVVFLALVLISGLTPLLRRLGIGRLPGDLHFRLFGREWF
                     IPLTSTLLLSALASLIARWL"
     gene            complement(2468..3580)
                     /gene="ribD"
                     /locus_tag="PK242_11585"
     CDS             complement(2468..3580)
                     /gene="ribD"
                     /locus_tag="PK242_11585"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOZ94703.1"
                     /translation="MLSALDHAMMRRALTLAERGRRLSPPNPAVGCVLVDAAATVLGE
                     GFTQPVGQAHAEVMALRDAAARGHRTQGATAYVTLEPCAHHGRTGPCCDALVAAGVRR
                     VVAALPDPNPRVGGQGLQRLRDAGVEVALGPPEVAAAARELNIGFFTRMERGTPWVRL
                     KVASSLDGRTALPDGRSQWITSPAARADAQAWRARACAVLTGIGTVLDDDPLLNVRLP
                     DSPRQPALVVLDSQLRTPAQARLWQVAGRRVCIATAVTDAARQQPLQARGAQVLALPG
                     DDGRVDLPALLAHLARQEVNELHVEAGHRLNGALLRAGLVDELLLYQAPRLLGDGVGI
                     ADLGPLAGLDQALAFDWHAIERVGPDLRLLARAPGR"
     gene            complement(3593..3904)
                     /locus_tag="PK242_11590"
     CDS             complement(3593..3904)
                     /locus_tag="PK242_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94704.1"
                     /translation="MPTFTRPAISLQQAVAETPTLAHLSRLATESAARLAVLRPLLPP
                     ALRPLVQAGAPEGDAWTLLAPHQAAAAKLRQLAPALQAALAAAGHPVSTLRVKVAQPL
                     R"
     gene            4116..>4806
                     /gene="secA"
                     /locus_tag="PK242_11595"
     CDS             4116..>4806
                     /gene="secA"
                     /locus_tag="PK242_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804245.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOZ94705.1"
                     /translation="MATILTKIFGSRNDRLLKQYRKTAAAINALEPALEKLSDDELRA
                     KTDEFRQRLAGGESLDKLLPEAFAVVREGSKRVMKMRHFDVQLVGAMALHEGKIAEMR
                     TGEGKTLTATLAVYLNALSGKGVHVVTVNDYLAGRDAEWMGRLYNFLGLTVGVNLPQM
                     ARADKQAAYQADITYGTNNEFGFDYLRDNMVYSTGERVQRGQHYAIVDEVDSILIDEA
                     RTPLIISGQAED"
BASE COUNT          665 a         1719 c         1794 g          628 t
ORIGIN      
        1 gcgcggcggc ggcgtcgcgc aggcgcacgc acagatcgac gtcgaaccac aggcgctgcg
       61 gcccctggcg ctcgaactcg tgcacaccga tgcgcaccgg cagttcgtgg cggcgcaggc
      121 tcagcaggcg gcagtccagc gccaggcgcg cgtccagcgc gccgaaggcc agcagcaggg
      181 aattcatcgg gtcagccctt cgtcgacggc aaacatcacg tcgcgcccca gcggcaccag
      241 gtgctgaccg ttgtcggcgc gcagggtgca gccgttcagg ctcatgttgt cggcgatgaa
      301 cagcgcgcag cgcgccacct cggccgcatc gatcggctgg cgcagcaggt tgacgcggct
      361 ggcacgcgca aagttgtcgg ccgtctgcgg cccgctcagg tacatcagcc cgggcgacag
      421 cccgcacacg cgcagccagg gcgccagcgc ctgcgcctgc agggccacgc tctgcgccag
      481 cgccagcttg ctgacggtgt aggaaaaata gtccgggttc aggttgtgca ccttctggtc
      541 cagcacgtgc accacactgt gccgcccccc gctgggctgg ccgcctgcgg ccaacaggct
      601 ggccagtgcg ttggccagca gcaggggcgt ggcgcagttg gtggcaaagt gctgtatcag
      661 gcccgccgcg cttgccgatc gggcgtcgtc tgcttcaaaa atggaagcat tgttgacgat
      721 gcagtgcggc ggcgcgccgt gcagcgtgga ggcctgccgc agcagcccgc aggccgcctc
      781 gggctgggcc aggtcggccc gcagcaggtg gcagcgccgg cccaggcgct cgacgtcggc
      841 ctgcagcgcc tgcgcctcgg cctcggcatg gcggtaatgc gccagcacgt cccagccagc
      901 gcgcgcgaac tgcaggctga tctcgcgccc gagccggcgc gcggcgccgg tgaccagcac
      961 ggcacggacg ggcagcgggg cggtgggcgc ggacatgggg cggcattgtc gggcaaaagg
     1021 cgcggcggca cgcgccggcg cgctgcggca tggcccacaa tgccgaccgt gcctgccgat
     1081 gccactgcca ctgcctcacc tgcgcccgac gcgctgccgg cgctgatcgc gcgcgaaatc
     1141 cgcgccgccg gcggctggtt gccgttcgag cgtttcatgc acctggcgct gtacgcgccg
     1201 gggctgggct actacgcccg ggcggcgcgc cagtttggcc agctgccgcg cagtggcagc
     1261 gacttcgtca ccgcgcccga gctgtcgccg ctgttcgccc gcgcgctggc ggtgcagctg
     1321 gccgacagcc tggcggccac cggcaccgat gaggtgtggg agttcggcgc cggctcgggc
     1381 gcgctggcgg ccgggctgat cgaggcgctg gacgcgctgg gccagccgct ggcgcgctac
     1441 accatcgtcg acgtctcggg cgcgctgcgg gcgcgccagc gccagcgcct ggccgcccat
     1501 ggcgcgcgcg tggcctgggc cgacgcgctg ccgccggtgc tgcgcggcgt ggtggtgggc
     1561 aacgaagtgc tggacgccat gccggtgacg ctgctggcgc ggcgcggcgg gcggtggttc
     1621 gagcgcgggg ttcgcattgg tgaatccatt gaggccctgg cgcaggatgg gcaagcgccg
     1681 gcagctatca aaacagaagc aatcggtttg acctgggaag accgcccgac cgcgctgcga
     1741 ccgccgctgg agccggccgg cacgcacgat ctggtgaccg agatccaccc gcaggcgcag
     1801 gccttcgtcg ccaccgtggc cgaggcgctg cggcgcggtg gcgccggcgc cgcctggttc
     1861 atcgactacg gctttccgga agccgagtac taccacccgc agcgcagcga gggcaccctg
     1921 gtgtgccacc gcgcgcaccg ggtggacgac cagccgctgg ccgacgtggg cgacaaggac
     1981 atcagcgccc acgtcaactt caccggcgtg gcgctggcgg cgcaggacgc cggcctggag
     2041 gtgctgggct acaccagcca ggggcgcttt ctgctcaact gtggcctggc cgaggcgctg
     2101 gccgcggccg acctgcccac gcgcgccaac gccatgaagc tgctggacga gcacgagatg
     2161 ggcgagctgt tcaaggtcat cggactggcc acgcccggcc acccgccggc gcgcggcttt
     2221 gcccagggcg accgcacgca ccggctgtga cacacatgat ccgctggctg atcgtcgtct
     2281 ttctggccct ggtgctgatc agcggcctga cgccgctgct gcgccggctg ggcatcggcc
     2341 ggctgccggg ggacttgcac tttcggctgt tcgggcgcga atggttcatt ccgctgacca
     2401 gcacgctgct gctgagcgcc ctggccagcc tgatcgcgcg ctggctgtag ggccgcgctg
     2461 aagcggctca gcgccccggc gcgcgcgcga gcagccgcag gtcgggcccg acgcgctcga
     2521 tggcgtgcca gtcgaacgcc agcgcctggt ccaggccggc cagcggcccc aggtcggcga
     2581 tgcccacgcc gtcgcccagc aggcgcggcg cctggtacag cagcagctcg tccaccaggc
     2641 cggcgcgcag cagggcgccg ttgagccggt ggccggcttc cacgtgcagc tcgttcacct
     2701 cctgccgggc caggtgcgcc agcagggccg gcagatcgac ccggccgtcg tcgcccggca
     2761 gcgccagcac ctgggcgccg cgcgcctgca gcggctgctg gcgtgcggcg tcggtgacgg
     2821 cggtggcgat gcacacgcgg cgcccggcca cctgccacag gcgcgcctgc gcgggcgtgc
     2881 gcagctggct gtcgagcacc accagcgccg gctggcgcgg gctgtcgggc aggcgcacgt
     2941 tgagcagcgg gtcgtcgtcc agcacggtgc cgatgccggt cagcacggcg caggcgcgtg
     3001 cgcgccaggc ctgagcgtcg gcgcgcgcgg cgggcgaggt gatccattgg ctgcggccgt
     3061 cgggcagggc ggtgcgcccg tccagggagc tggcgacctt cagccgcacc cagggcgtgc
     3121 cgcgctccat gcgggtgaaa aagccgatgt tcagctcgcg cgccgccgcg gccacttcgg
     3181 gcggccccag cgccacctcg acgccggcgt cgcgcagccg ctgcaggccc tggccgccga
     3241 cgcgcgggtt cgggtccggc agcgcggcca ccacgcggcg cacgccggcc gcgaccagcg
     3301 cgtcgcagca cggcccggtg cggccgtggt gtgcgcaggg ctccagcgtc acgtaggcgg
     3361 tggcgccctg cgtgcggtgc ccgcgcgcgg cggcatcgcg cagcgccatc acctcggcat
     3421 gcgcctggcc gaccggctgc gtgaagcctt cgccgagcac cgtggcggcg gcgtccacca
     3481 gcacgcagcc gaccgccggg ttgggcggcg acaggcgccg accgcgctcg gccagcgtca
     3541 gcgcgcggcg catcatggcg tggtcaaggg cgctcagcat ggtcgccgcc cgtcagcgca
     3601 ggggctgcgc caccttgacg cgcagcgtgg ataccgggtg gccggccgcc gccagcgccg
     3661 cctgcagcgc cggcgccagc tggcgcagct tggccgccgc cgcctggtgc ggcgccagca
     3721 gcgtccaggc gtcgccctcg ggcgcgccgg cctgcaccag cggccgcagt gccggcggca
     3781 gcagtggccg cagcaccgcc aggcgggcgg ccgattcggt ggccagccgg ctcaggtggg
     3841 ccagcgtggg cgtctcggcc acggcctgtt gcagcgagat ggcgggacgg gtgaaagtgg
     3901 gcatcgacaa gggcaaaaac cgcagcgtgg cgggcattat ccgcgccgct ggcgggcagc
     3961 taaaatgctt ggttgccacg cgaaacaccg ggcgcgctgc ctgcggggcc ttccgcaggt
     4021 ggtcgctgcc ggccgggttc gcgtcatttt gacctttttg ggacagtcga tcaccgtgcg
     4081 gtgcgccagt ggggcgtcgc ggtgaccgcg cacgcatggc caccatcctc accaagattt
     4141 tcggcagccg caacgaccgc ctgctcaagc aataccgcaa gaccgccgcc gccatcaacg
     4201 cgctggagcc ggcgctggag aagctgagcg acgacgagtt gcgcgccaag accgacgaat
     4261 tccgtcagcg cctggcgggc ggcgaaagcc tggacaagct gctgcccgag gcctttgcgg
     4321 tggtgcgcga aggctccaag cgtgtcatga agatgcgcca cttcgacgtg cagctggtcg
     4381 gcgccatggc gctgcacgag ggcaagatcg ccgagatgcg taccggcgag ggcaagaccc
     4441 tgaccgccac gctggcggtg tacctgaacg cgctgtcggg caagggcgtg cacgtggtca
     4501 ccgtgaacga ctacctggcg ggccgcgacg ccgaatggat gggccggctg tacaacttcc
     4561 tggggctgac ggtgggggtc aacctgccgc agatggcgcg cgcggacaag caggccgcct
     4621 accaagccga catcacctac ggcaccaaca acgaattcgg cttcgactac ctgcgcgaca
     4681 acatggtcta ttcgaccggt gagcgggtgc agcgcggcca gcactacgcc atcgtcgacg
     4741 aggtggactc catcctgatc gacgaggcgc gcacgccgct gatcatcagc ggccaggccg
     4801 aggacc
//
LOCUS       DAOOOG010000189         4755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_21618_length_4755_cov_25.348511, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000189 DAOOOG010000000
VERSION     DAOOOG010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21618_length_4755_cov_25.348511"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..3626
                     /locus_tag="PK242_11600"
     CDS             201..3626
                     /locus_tag="PK242_11600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOZ94706.1"
                     /translation="MPEPSRKQPEAQAEAAARVRRLPRRSVAQPAPAYRPPYAELHCL
                     SHFSFQRGASSPEELVRRAWALGYTALAITDECSVAGAVRAWQALGELERSLAAADAD
                     PEFAPLHAARAAQGATEPLRLIYGAEFRFAGQGTLVALARDLAGWGALCGFITRCRGA
                     APKGQYALPPLAEALAALPGCERLWAPARAAGMAPSPLWGEGGGEGPATGVALLLELH
                     AGADDALWLAQLRALADRLGCPLVAAGDVQMHVRARKRLHDVLCAIGLGRPLHDCGFA
                     LQPNAERHLRPRARLARIYPPDTLAATLALAGRCRLRLEQLRYHYPRESVLPGLTPTE
                     TLRRHTIEGARRRLPRGVPRQIWRYVARELALIAECRYEMFFLTVHDIVREARRRGIL
                     CQGRGSAANSVVCWCLGITEADPRFSHPLLERFISRARRHEPPDIDVDFEHERREEII
                     QYIYARYGRARAALAAAVITWRTRSALRDVGAALGVDQRLIDAVASDHHGFDAQGVAT
                     RVDGLAAGLGLQPAPERISLWLELAAQLKGAPRHLSQHVGGFVLTETPLTQLVPVEPA
                     RMAGRSVIQWDKDDLEALGMLKVDVLALGMLTAIRRCLDLASRWQGAPLTRAHIVPED
                     AATYAMIRRADTVGVFQIESRAQMSMLPRLAPTTFYDLVVQVAIVRPGPITGGMVHPY
                     LKARERARRGLPIEYERSHLDAPGQPPRLARALARTLGVPIFQEQVMELSMLAAGFSA
                     EQADRLRRAMAAWKRKGGLEPFERALTDGMLARGYSQDFAHRIFEQIKGFGEYGFPES
                     HAYSFALIAYESAWLKCHQPEAWLAALLNSQPMGFYAPAQLVQDARRHGVVVLPADVS
                     ASHWDCTLEAQASRSTIEEQNMPPALIQQAPAAIKIDGSGPPRPAVRLGLRQVAGLAE
                     AAARRIVEARAGQAFASVDDLARRARLDAGDLNRLAAADALASLAGHRRQQVWQGAAW
                     RAAPALLHEAPIDEAPLALPPAPEGEAIVHDYAALGLTLRRHPLALLRARLSRWRLLT
                     AEQLARTPHGRSVRACGLVVGRQRPATAKGTIFVTLEDETGPVNVIVWPGLREDEGQR
                     NALLRARLLAVEGQWQWGGGSGGAVRHLVARRLRDLTPLLGRLAGLTTSRDFH"
     gene            3792..4355
                     /gene="thpR"
                     /locus_tag="PK242_11605"
     CDS             3792..4355
                     /gene="thpR"
                     /locus_tag="PK242_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOZ94707.1"
                     /translation="MTTEPASPAAPQRLFTALFPPPAACAAIDAERRRWAGLPRRLRP
                     VPERMHLTLQFHDRVAPPQAQAWQAALATLRFEPFDIALVRAELWHAPHGTLAVLRAA
                     PHPALTALHERTAELAAQAGLPREPRAYKPHLTALRQAERVTLAALAAPIAWRVQAVE
                     LIWSDLLARPPRYHRLGLFAAAERPQD"
     gene            4621..>4755
                     /locus_tag="PK242_11610"
     CDS             4621..>4755
                     /locus_tag="PK242_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOZ94708.1"
                     /translation="MSATAVGLFEAKTHLSEYVARAERGEEVIITRHNKPVAKIVPLH
                     A"
BASE COUNT          602 a         1762 c         1755 g          636 t
ORIGIN      
        1 cgccgggccg ctcccaaggc gggcggcggc ccccttgggg ggcagcgcag caagcgcagc
       61 tgcggagcgt gggggccctg tctctcgtga ttactacgtg gcgcgcaacc cggcgtccga
      121 gctgttgtgg gtgtttcgcg agcgcccgac ccgcgccggc ccgcaggcgg gtcagacgcg
      181 ctggtttctg cacggccgtt atgcctgagc ccagccgcaa gcagcccgag gcgcaggccg
      241 aagcggcggc gcgggtgcgg cgcctgccgc gtcggtcggt ggcgcagccg gcgccggcgt
      301 accggccgcc ctacgccgag ttgcactgtc tgagccactt cagcttccag cgcggcgcca
      361 gcagccccga agagctggtg cgccgcgcct gggcgctggg ctacacggcg ctggcgatca
      421 ccgacgaatg ctcggtggcg ggcgcggtgc gggcctggca ggcgctgggc gagctggagc
      481 gctcgctcgc cgcggccgac gccgaccccg aattcgcccc gctgcacgcc gcgcgcgccg
      541 cgcagggcgc gaccgagccg ctgcggctga tctacggcgc cgagttccgc tttgccggcc
      601 agggcacgct ggtggcgctg gcgcgcgacc tggcgggctg gggcgcgctg tgcggcttca
      661 tcacgcgctg ccgcggcgcg gcgcccaagg ggcagtacgc gctgccgccg ctggccgagg
      721 cgctggcggc gctgccgggt tgtgaacggc tgtgggcgcc ggcgcgcgcg gccgggatgg
      781 ctccctcgcc cctctgggga gagggtgggg gtgaggggcc ggcgacaggc gtcgccctgc
      841 tgctggagct gcacgccggc gccgacgacg cgctgtggct ggcgcagctg cgcgcgctgg
      901 ccgatcgtct gggctgcccg ctggtggccg ccggcgacgt gcagatgcac gtgcgcgcgc
      961 gcaagcgcct gcacgatgtg ctttgcgcca tcggcctggg ccggccgctg cacgactgcg
     1021 gcttcgcgct gcagcccaac gccgagcgcc acctgcgccc gcgcgcgcgg ctggcgcgca
     1081 tctacccgcc ggacaccctg gccgctacgc tggcgctggc cgggcgctgc cgcttgcggc
     1141 tcgagcagct gcgctaccac tacccgcgcg agagcgtgct gcccggcctg acgccgaccg
     1201 aaacgctgcg ccgccacacc atcgagggcg cgcgccggcg cctgccccgc ggcgtgccgc
     1261 ggcagatctg gcgctacgtg gccagggaac tggcgctgat cgccgagtgc cgctacgaga
     1321 tgttcttcct caccgtgcac gacatcgtgc gcgaggcgcg gcgtcgcggc atcctgtgcc
     1381 aggggcgcgg ctcggccgcc aactcggtgg tctgctggtg cctgggcatc accgaggccg
     1441 acccgcgctt ctcgcacccg ctgctcgagc gcttcatcag ccgggcgcgc cgccacgaac
     1501 cgcccgacat cgacgtcgac ttcgagcacg agcggcgcga ggagatcatc cagtacatct
     1561 acgcccgcta cggccgcgcg cgcgccgcgc tggccgccgc cgtcatcacc tggcgcacgc
     1621 gcagcgcgct gcgggacgtc ggcgcggcgc tgggggtgga ccagcgcctg atcgacgccg
     1681 tcgccagcga ccaccacggc ttcgacgccc agggcgtggc cacgcgcgtg gacggcctgg
     1741 ccgccggcct ggggctgcaa ccggcgcccg agcggatcag cctgtggctg gaactggcgg
     1801 cgcagctcaa gggcgcgccg cggcatctga gccagcacgt gggcggcttc gtgctgaccg
     1861 aaacgccgct gacgcaactg gtgccggtcg agcccgcccg catggccggg cgcagcgtca
     1921 tccagtggga caaggacgac ctggaggcgc tgggcatgct caaggtcgac gtgctggcgc
     1981 tgggcatgct gacggcgatc cgccgctgcc tcgatctggc cagccgctgg cagggcgcgc
     2041 cgctgacgcg cgcgcacatc gtgcccgagg acgccgccac ctacgccatg atccggcgcg
     2101 ccgacaccgt cggcgtgttc cagatcgaaa gccgggcgca gatgagcatg ctgccgcgcc
     2161 tggcgcccac caccttctac gacctggtgg tgcaggtggc gatcgtgcgc ccggggccga
     2221 tcaccggcgg catggtgcac ccctacctga aggcgcgcga gcgcgcccgc cgcggcctgc
     2281 cgatcgagta cgagcgctcg cacctggacg cccccggcca gccgccgcgc ctggcgcggg
     2341 cgctggcgcg cacgctcggc gtgccgatct tccaggagca ggtgatggag ctgtcgatgc
     2401 tcgccgccgg cttctccgcc gagcaggccg accgcctgcg ccgcgccatg gcggcctgga
     2461 agcgcaaggg cgggctcgag ccgttcgagc gcgcgctcac cgacggcatg ctggcgcgcg
     2521 gctacagcca ggatttcgcg caccgcatct tcgagcagat caagggcttt ggcgaatacg
     2581 gctttcccga aagccacgcc tacagctttg ccctgatcgc ctacgagagc gcctggctca
     2641 agtgccacca gcccgaggcc tggctggcgg cgctgctcaa ctcgcaaccc atgggcttct
     2701 acgccccggc gcagctggtg caggacgcgc gccgccacgg cgtcgtggtg ctgccggccg
     2761 acgtgtcggc cagccactgg gactgcacgc tggaggcgca ggcgagcaga tcgaccattg
     2821 aagaacaaaa tatgcctcca gcgcttatcc agcaagcgcc ggcagctatc aaaattgatg
     2881 gttccggccc gccgcgcccg gccgtgcgcc tgggcctgcg gcaggtggcg ggcctggccg
     2941 aggccgccgc gcggcgcatc gttgaggcgc gggctggaca agcttttgcc agcgtcgacg
     3001 acctggcgcg ccgggcgcgc ctggatgccg gcgatctgaa ccggctcgcc gccgccgacg
     3061 ccctggcgag cctcgccggc caccggcgcc agcaggtctg gcagggcgcc gcctggcgcg
     3121 ccgccccggc gctgctgcac gaggcgccga tcgacgaggc gccgctggcg ttgccgccgg
     3181 cacccgaggg cgaggccatc gtgcacgact acgcggccct gggcctgacg ctgcgccgcc
     3241 acccgctggc gctgctgcgc gcgcgccttt cgcgctggcg cctgctgacg gccgagcagc
     3301 tggcgcgcac gccccatggc cgcagcgtgc gcgcctgcgg cctggtggtc gggcgccagc
     3361 ggccggccac cgccaagggc accatcttcg tcaccctgga agatgaaacc gggccggtca
     3421 acgtcatcgt ctggcccggc ctgcgcgaag acgagggcca gcgcaacgcc ctgctgcgcg
     3481 cgcgcctgct ggcggtcgaa ggccagtggc agtggggcgg cggcagcggc ggcgcggtgc
     3541 gccacctggt ggcgcgccgc ctgcgcgacc tcacgccgct gctgggccgg ctggccgggc
     3601 tgaccacgag ccgggacttt cattgaacct ggtgtggcgt tgcgcgccat ctggaacatg
     3661 aaaccgcgtc ttctcgccca tgatgccttt gcaaagccgc agcgtgcccg tatcgggcgg
     3721 acagcgccga tccgatttca gtgtttcagt ggactgggtt gaactgggcg tcggcgcgtc
     3781 acaatgccgg tgtgaccacc gaacctgcct cgcccgctgc gccgcagcgc ctgttcacgg
     3841 cgctgtttcc gccgcctgcg gcctgtgcgg ccatcgacgc cgagcgccgg cgctgggctg
     3901 gcctgccgcg ccgcctgcgg ccggtgcccg agcgcatgca cctgacgctg cagtttcacg
     3961 accgggtggc accaccgcag gcgcaggcct ggcaggcggc cctggccacg ctgcgcttcg
     4021 agccgttcga catcgccctg gtgcgcgccg aactgtggca cgccccgcat ggcaccctgg
     4081 cggtgctgcg cgcggcgccc cacccggccc tgacggcgct gcatgaacgc accgccgaac
     4141 tggcggcgca ggccggcctg ccacgcgagc cgcgcgcgta caaaccgcac ctgacggcgc
     4201 tgcgccaggc cgagcgcgtg acgctggcgg cgctggcggc gcccatcgcc tggcgcgtgc
     4261 aggcggtcga gctgatctgg tccgacctgc tggcgcggcc gccgcgctac caccggctgg
     4321 gcctgtttgc cgccgccgaa aggccgcagg actgacgggc ggcaccgccc gcatcggccc
     4381 atgatctgcc ctgccagagg atcggctgcc gacaaaggca tcggcttgca gcgcacatgg
     4441 ggcaagcgcg ggcagctatg gtttctgatg gatcgggcgc gggccgaggc acggtgtgca
     4501 gcgcgctgcg cggcccatgg aacgcccgag gcgggtgcgc ctgcgcgggc tgagtctcgg
     4561 cgcgggcaat ggggctaaaa tctggttgat tcaaccagtt tgaatgtcag ggagtccgcc
     4621 atgtccgcca ccgccgtggg cctgttcgag gccaagaccc acctcagcga gtacgtcgcc
     4681 cgcgccgagc gaggcgagga ggtgatcatc acccgccaca acaagccggt ggccaagatc
     4741 gtgccgctgc atgcc
//
LOCUS       DAOOOG010000190         4722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_21815_length_4722_cov_30.616027, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000190 DAOOOG010000000
VERSION     DAOOOG010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21815_length_4722_cov_30.616027"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PK242_11615"
     CDS             <1..394
                     /locus_tag="PK242_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOZ94709.1"
                     /translation="LAFFVYVPAFALLAAAGLLPTRLAQAPWGELLFQALFQGVGSVV
                     ISGITFVQMVRVFGPVRSTMITALVPGLSALGAVLLLGEPLGLNLLAGLALVTAGILF
                     GVRAARPVTPDSIAVGARQTSAGAVKAS"
     gene            391..1119
                     /gene="tsaB"
                     /locus_tag="PK242_11620"
     CDS             391..1119
                     /gene="tsaB"
                     /locus_tag="PK242_11620"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOZ94710.1"
                     /translation="MNQTAFNLLAFDTSTEQLSVAVQRGADGRRWAHQGAGGAQASVT
                     LIPTVQRLLAEAGLALGDLHAIVFGRGPGSFTGLRTACAVAQGLAWGAGLPVLPLDTL
                     LAVAEAARQSAGATRVLALLDARMGEVYHAAYAWSADHGWQTLAEPAVCAPERLPAPP
                     PGAVLAGNAMAVHGERLPPALRALPQVHALPGAQALLTLAPAGLTAGRAVPADQALPL
                     YIRDKVAQTTAERAAARSAPAQTP"
     gene            1116..1616
                     /gene="rimI"
                     /locus_tag="PK242_11625"
     CDS             1116..1616
                     /gene="rimI"
                     /locus_tag="PK242_11625"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOZ94711.1"
                     /translation="MSTLLSPPSPAPAAALEAHFAPMTPERLDAVLAVEQSAYSHPWS
                     RGNFRDSLAAGHQAQCLLAGDELLGYFVAMLGHREVHLLNITVAPVHQRQGWALVMLD
                     ALALWSRARQAEWLWLEVRASNTRAQQVYLRHGFARVGLRKGYYPDGHGLREDAVVMN
                     LRLEPA"
     gene            1642..2556
                     /locus_tag="PK242_11630"
     CDS             1642..2556
                     /locus_tag="PK242_11630"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOZ94712.1"
                     /translation="MTLALDARQRAMLAEMGIRLWQRAPQPVTLEAPETIAAAADEAR
                     PSKFFDAKSAGGAAPPPMTPRRMAEAAAPAPAPAAAPASALGPRPEGVEAMDWPQLQA
                     AVATCRACGLCAGRTQTVFGAGSRRADWMVVGEAPGETEDRQGQPFVGPAGQLLDAML
                     AAIGLSRLADDAMNSVAFGAEPADAAGPNGLKRGVYIANVLKCRPPGNRNPQPDEIAQ
                     CEPYLRRQVALVQPKVILAMGRFAVQSLLQTSEPIGRLRGRVHHYAGVPVIVTYHPAY
                     LLRALPDKAKAWQDLCLALEVMRGSAPG"
     gene            complement(2731..>4722)
                     /locus_tag="PK242_11635"
     CDS             complement(2731..>4722)
                     /locus_tag="PK242_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOZ94713.1"
                     /translation="GTEVRFLPDTEIFKENHDFHYDILAKRLRELSFLNHGVRIRLID
                     ERSGKEDDFSGAGGVKGFVDFINGSKKVLHPNAFYASGARPAESYGGIAGTEIGVEVA
                     MQWNDGYNEQVLCFTNNIPQRDGGTHLTGLRAAMTRVITKYIADNELAKKAKVDVSGD
                     DLREGLVCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEDIVGKALAEFLEERPNDAKIV
                     CGKIVEAARAREAARKAREMTRRKGVLDGFGLPGKLADCQEKDPAQCEIYIVEGDSAG
                     GSAKQGRDRKFQAILPLRGKILNVERARYEKLLTSNEIVTLITALGTGIGKTAADSGR
                     SGADDFNIDKLRYHRVIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLY
                     KVKNGKEELYLKDGAALDAFLLRVALKDAVVHTGGAAPRQLSGDTLDELARRHQLAEA
                     VIERLSAFMDTEALRAIAAGVTLNLDTVEAAQASAAVLQQHLHAEQANGSAPEVSGEF
                     DPRSDKPVLRISRRHHGNVRHSVITQDFVHGADYAALAEAAQTFRDLLGEGARVRRGE
                     GERQREQAVASFREAMAWLIGEAERTTTRQRYKGLGEMNPEQLWETTMDPAVRRLLRV
                     QIEDAIEADRVFTTLMGDEVEPRRAFIETNALRALNIDA"
BASE COUNT          575 a         1670 c         1705 g          772 t
ORIGIN      
        1 gctggcgttc ttcgtctacg tgccggcgtt cgccctgctg gccgctgccg ggctgctgcc
       61 cacgcgcctg gcgcaggcgc cgtggggcga gctgctgttt caggcgctgt tccagggcgt
      121 cggctcggtg gtgatttcgg gcatcacctt cgtgcagatg gtgcgcgtgt tcgggccggt
      181 gcgctcgacc atgatcaccg ccctggtgcc cgggctgtcg gcgctggggg cggtgctgct
      241 gctgggcgag ccgctgggcc tgaacctgct ggccgggctg gcgctggtga ccgccggcat
      301 cctgttcggg gtgcgggcgg cgcgcccggt cactcctgat tcgatagctg ttggcgcacg
      361 ccagacaagc gctggagccg taaaagcctc atgaaccaga cggccttcaa cctgctggcc
      421 ttcgacacca gcaccgagca gctgtcggtg gcggtgcagc gcggcgccga cggccgccgc
      481 tgggcgcacc agggcgccgg cggggcgcag gcctcggtca cgctgatccc cacggtgcag
      541 cggctgctgg ccgaagccgg gctggcgctg ggcgatctgc acgccatcgt gttcgggcgc
      601 gggccgggct cgttcaccgg gctgcgcacc gcctgcgcgg tggcgcaggg gctggcctgg
      661 ggtgccggcc tgccggtgct gccgctggac accctgctgg cggtggccga agcggcgcgc
      721 cagtcggccg gcgccacccg ggtgctggcg ctgctggacg cgcgcatggg cgaggtctac
      781 cacgccgcct acgcctggtc cgccgaccac ggctggcaga ccctggccga gccggcggtc
      841 tgcgcgcccg agcggctgcc ggcgccgccg cccggggcgg tgctggccgg caacgccatg
      901 gccgttcatg gcgagcgcct gccgcccgcg ctgcgggcgc tgccgcaggt gcacgccctg
      961 cccggcgccc aggcgctgct gacgctggcg ccggccggcc tgaccgccgg tcgggcggtg
     1021 ccggccgatc aggcgctgcc gctctacatc cgcgacaaag tggcgcagac tacggccgag
     1081 cgcgctgccg cccgcagcgc cccggcccag accccatgag caccctgctg tcgccccctt
     1141 cccccgcccc cgcggccgca ctggaggccc atttcgcccc catgacgccc gagcggctgg
     1201 acgccgtgct ggcggtcgag caaagcgcct acagccaccc ctggtcgcgc ggcaacttcc
     1261 gcgacagcct ggccgccggc caccaggcgc agtgcctgct ggcgggcgac gagctgctgg
     1321 gctacttcgt cgccatgctc ggtcatcgcg aggtgcacct gctcaacatc acggtggcgc
     1381 cggtccacca gcgccagggt tgggcgctgg tgatgctgga cgcgctggcg ctgtggtcgc
     1441 gcgcgcgcca ggccgaatgg ctgtggctgg aagtgcgcgc cagcaacacc cgcgcgcagc
     1501 aggtgtacct gcgccacggc tttgcccgcg tcggcctgcg caagggctac taccccgacg
     1561 gccacggcct gcgcgaagac gccgtggtca tgaacctgcg cctggagccc gcctgatggc
     1621 gccgtcgccg cggatcgttc catgaccctg gcgctggacg cacgccagcg cgccatgctg
     1681 gccgaaatgg gcatccggct gtggcagcgc gcgccccagc ccgtcaccct ggaagctcct
     1741 gaaacaatag ctgctgccgc agatgaggcg cggcctagca agttttttga cgcaaaatcc
     1801 gcggggggcg cggcgccccc cccgatgacc ccacggcgca tggccgaagc ggccgccccg
     1861 gcgccagcgc cggccgccgc gcccgccagc gccctgggcc cgcgcccgga aggcgtggag
     1921 gccatggact ggccgcaact gcaggccgcc gtcgccacct gccgcgcctg cggcctgtgc
     1981 gccgggcgca cccaaaccgt gttcggcgcc ggcagccggc gcgccgactg gatggtggtg
     2041 ggcgaagccc cgggcgaaac cgaggaccgc cagggccagc ccttcgtcgg ccccgccggc
     2101 cagttgctgg acgccatgct ggccgccatc gggctctcgc ggctggccga cgacgctatg
     2161 aattcagtag ctttcggcgc tgaaccagcg gacgctgcag gccccaacgg cttgaagcgc
     2221 ggggtctaca tcgcgaacgt cctgaagtgc cgcccgcccg gcaaccgcaa cccgcagccg
     2281 gacgaaatcg cccagtgcga accctatctg cgccgccagg tcgcgctggt gcagcccaag
     2341 gtgattctgg ccatgggccg ctttgccgtg cagtcgctgc tgcagaccag cgagcccatc
     2401 ggccgcctgc gcggccgcgt gcaccactac gccggcgtgc ccgtgatcgt cacctaccac
     2461 ccggcctacc tgctgcgcgc cctgcccgac aaggccaagg cctggcagga cctttgcctg
     2521 gcgctggagg tgatgcgcgg cagcgccccg ggctgaagcg ggctcgcttt gaaagacgaa
     2581 tgacctcgtt gcgcttcgat gacccatggg acatgggtca agccgatgca gccgctgtcg
     2641 ttggtccgct tgttcttcgc gaagccacag gaggcggggc cccggatccc cggatggcgc
     2701 gcggcgccag ccggccggtg tcgggggccc tcaggcgtcg atgttcagcg cccgcagcgc
     2761 gttggtttcg atgaaggcgc ggcgcggctc gacttcgtcg cccatcaggg tggtgaagac
     2821 gcggtcggct tcgatggcgt cttcgatctg cacgcgcagc aggcgccgca ccgccgggtc
     2881 catggtggtt tcccacagct gctccgggtt catttcgccc aggcccttgt agcgctggcg
     2941 cgtggtggtg cgctcggcct cgccgatcag ccaggccatg gcctcgcgga agctggccac
     3001 cgcctgctcg cgctggcgct cgccttcgcc gcggcgcacg cgcgcgcctt cgcccagcag
     3061 gtcgcggaag gtctgcgccg cttcggccag ggcggcgtag tcggcgccgt gcacgaagtc
     3121 ctgcgtgatg acgctgtggc ggacgttgcc gtggtggcgg cggctgatgc gcagcaccgg
     3181 tttgtcgctg cgggggtcga attcgcccga cacttcgggc gcgctgccgt tggcctgctc
     3241 ggcgtgcagg tgctgctgca gcacggcggc cgaggcctgg gccgcttcca ccgtgtccag
     3301 gttcagggtc acgccggcgg cgatggcgcg cagcgcctcg gtgtccatga aggccgacag
     3361 acgctcgatc acggcttcgg ccagctggtg gcggcgcgcc agctcgtcga gggtgtcgcc
     3421 gctcagttgc cgcggggcgg cgccgccggt gtgcaccacg gcgtccttca gcgccacgcg
     3481 cagcaggaag gcgtccagcg cggcgccgtc cttcaggtac agctcttcct tgccgttctt
     3541 gaccttgtac agcggcggct gggcgatgta gatgtggccg cgctcgacca gctcgggcat
     3601 ctggcggtag aagaaggtga gcagcagggt gcggatgtgg gcgccgtcga cgtcggcgtc
     3661 ggtcatgatg atgacgcggt ggtagcgcag cttgtcgatg ttgaagtcgt cggcgccgct
     3721 gcggcccgaa tcggccgcgg tcttgccgat gccggtgccc agggcggtga tgagcgtgac
     3781 gatctcgttg ctggtcagca gcttttcgta gcgcgcgcgc tcgacgttca ggatcttgcc
     3841 gcgcagcggc aggatggcct ggaacttgcg gtcacgcccc tgcttggcgc tgccgccggc
     3901 gctgtcgccc tcgacgatgt agatctcgca ctgggccggg tccttttcct ggcagtcggc
     3961 cagcttgccg ggcaggccga agccgtcgag cacgcccttg cgccgcgtca tctcgcgcgc
     4021 cttgcgggcg gcttcgcggg cgcgcgcggc ctcgacgatc ttgccgcaga cgatcttggc
     4081 gtcgttgggg cgctcttcca ggaattcggc cagcgccttg ccgacgatgt cttcgaccgg
     4141 cgcgcgcacc tcgctggaca ccagcttgtc cttggtctgg ctgctgaact tgggctcggg
     4201 caccttgacg ctgagcacgc acaccaggcc ttcgcgcagg tcgtcaccgc tcacatcgac
     4261 cttggccttc ttggccagct cgttgtcggc gatgtacttg gtgatcacgc gcgtcatggc
     4321 ggcgcgcagg cccgtgaggt gggtgccgcc gtcgcgctgc gggatgttgt tggtgaagca
     4381 cagcacctgc tcgttgtagc cgtcgttcca ctgcatggcc acttcgacac cgatttcggt
     4441 gccggcgatg ccgccgtagc tttcggccgg gcgcgcaccg ctggcgtaga aggcgttcgg
     4501 gtgcagcacc ttcttgctgc cgttgatgaa gtcgacgaag cccttgacgc cgccggcgcc
     4561 ggaaaagtcg tcttccttgc cgctgcgctc gtcgatcagg cggatgcgca cgccgtggtt
     4621 gaggaaggac agctcgcgca ggcgcttggc caggatgtcg tagtggaagt cgtggttttc
     4681 cttgaagatc tcggtgtcgg gcagaaagcg cacctcggtg cc
//
LOCUS       DAOOOG010000191         4669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_22128_length_4669_cov_24.029909, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000191 DAOOOG010000000
VERSION     DAOOOG010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22128_length_4669_cov_24.029909"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PK242_11640"
     CDS             complement(<1..686)
                     /locus_tag="PK242_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ94714.1"
                     /translation="MKPSTDPNDALMARARRVIPGGVNSPVRAFRAVGGTPRFVQRAQ
                     GPYFWDAAGTRYIDYIGSWGPMILGHGDPAVLEAVQKAAAEGFSFGAPTEREIELAEE
                     ILSLRPGMEMLRLVSSGTEAGMTAIRLARGATGRSAIIKFEGCYHGHADALLVKAGSG
                     LATFGNPTSAGVPPEVVQHTMVLEYNNLAQLEQAFAEHGPQIAGLIMEPIAGNMNFVR
                     ASVDFTRRCR"
     gene            complement(670..1542)
                     /locus_tag="PK242_11645"
     CDS             complement(670..1542)
                     /locus_tag="PK242_11645"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOZ94715.1"
                     /translation="MPNVLSFNAGDPTGAGGSGSDATVIAAIGAHALPVVTGVYVRDT
                     RETMSHCAIDEAAVAEQAAAVAEDVPLHVIKVGFVGSAEAVSAIAAFTSDYASVPVVA
                     YMPDLSWWSEAEIDHYLDACRELLLPQTTVLVGNNATLRHWLLPESTTDRHARATDLA
                     RAAAERGVAFVLVTGLPLAGDRLGNVLASPEAELFSQSFERIDADFLGAGDILSAALA
                     ALIATGSELTEATSEALQYLDQALDHGFRPGMGRALADRLFWAASDADDQADTADDGP
                     ALPPFPEPFNETKH"
     gene            1715..1885
                     /locus_tag="PK242_11650"
     CDS             1715..1885
                     /locus_tag="PK242_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOZ94716.1"
                     /translation="MNPKTWMCVICGWLYDEAAGAPDHGIAPGTPWEQVPADWCCPEC
                     GAHKLDFEMVQI"
     gene            1965..2351
                     /locus_tag="PK242_11655"
     CDS             1965..2351
                     /locus_tag="PK242_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ94717.1"
                     /translation="MTSRRVLVIDDSNTIRRSAEIFLKQGGHEVMLAEDGFDALAKVN
                     DAQPDLIFCDILMPRLDGYQTCAIIKRNEKYSSIPVVMLSSKDGVFDKARGRMVGSQD
                     YLTKPFTKDQLLQAVQQFGNANQGAV"
     gene            2351..2716
                     /locus_tag="PK242_11660"
     CDS             2351..2716
                     /locus_tag="PK242_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ94718.1"
                     /translation="MSIRNVLIVDDSKTELLHLSEMLKKAGYAVSTAENAEEALRRLE
                     QAKPDLILMDVVMPGQNGFQLTRAISRDPRYADVPIIMCTSKNQETDRVWGMRQGARD
                     YVTKPVDQAELMGKIRALG"
     gene            2722..3255
                     /locus_tag="PK242_11665"
     CDS             2722..3255
                     /locus_tag="PK242_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOZ94719.1"
                     /translation="MSNKQALREFQSRLAARLQSAQTGGVAASWLAVEAGSQRLLFPL
                     SHAGEIFSWTDVQRVPYVQPWFLGVANLRGGLSGVIDLARFVGGAEPVAGRSEADLAQ
                     CRLVALNPVLGANCALLVDRLLGLRTTEAFASSESADPIAPAFFGHRYADADGQSWQE
                     LNLQLLSQHPAFLGIGV"
     gene            3277..>4669
                     /locus_tag="PK242_11670"
     CDS             3277..>4669
                     /locus_tag="PK242_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HOZ94720.1"
                     /translation="MALASQLTNLFRSKSQDSSTGGDEAAVHEELLSTPPTSHADDSR
                     AAAESSVMVSAIDDSDDGATPGDDVFSVPLLGTRTAAQHQRLLSILLGLALFALVIVG
                     FWTVSRTDKGARQVGAIGQSLMQSQRLAKSVSQALVGSPEAFGEVKESAAALSANVRG
                     LQDGSDVVPALSDAFSDELGKIGPLAQRADKSAAVVMAQQQILTQVGAALRAVSRQST
                     ELLEVAETVSSLRLQQNASVSEISAAGQLVMLTQRIGKSANEFLTIEGVSPEAVFLLG
                     KDLNTFKEIAEGLLNGSADLRLAPARDPQTRERLTELLKLYEETRTQAGGVLGNLQGL
                     VAAREAQTTIVSDSEPLRKALEGLQDKLSGTSGVGFGNLMMLVIPAILLLLAAAGLVA
                     VQLRDSRKRQTLAEQQRRSAEQDQQEAKRVNDANQAAILRLMNELQTVAEGDLTQEAT
                     VTEDITGAIADSVN"
BASE COUNT          813 a         1417 c         1602 g          837 t
ORIGIN      
        1 tcgcggcagc ggcgcgtgaa gtcgacgctg gcgcgcacga agttcatgtt gccggcaatc
       61 ggctccatga tcaggccggc gatctgcggc ccgtgctcgg caaaggcctg ttccagctgc
      121 gccaggttgt tgtattccag caccatggtg tgctgcacca cctcgggcgg cacgccggcg
      181 ctggtcgggt tgccgaaggt ggccaggccg gagccggcct tgaccagcag ggcgtcggcg
      241 tggccgtggt agcagccctc gaatttgatg atggcgctgc gtccggtggc gccgcgcgcc
      301 aggcggatgg cggtcatgcc ggcttcggtg ccgctggaga ccaggcgcag catctccatg
      361 ccggggcgca ggctgaggat ttcctcggcc agctcgatct cgcgctcggt gggcgcgcca
      421 aaggaaaagc cctcggcggc ggccttctgc accgcttcca gcacggccgg gtcgccgtgg
      481 cccaggatca tcgggcccca ggagccgatg tagtcgatgt agcgtgtgcc cgccgcgtcc
      541 cagaagtagg ggccctgggc gcgctgcacg aagcgcggcg tgccgccgac ggcgcgaaag
      601 gcccgcacgg gcgagttgac gccgccggga atcacgcggc gggcgcgcgc catcagcgcg
      661 tcgttggggt cagtgcttgg tttcattgaa gggttccggg aaagggggaa gggctgggcc
      721 gtcgtcggca gtgtccgcct ggtcgtcggc atcggaggcg gcccaaaaca gccggtcggc
      781 cagcgcccgg cccatgccgg ggcgaaagcc gtggtccagg gcctggtcca ggtattgcag
      841 ggcttcgctg gtcgcttcgg tgagctcgct gccggtggcg atcagcgccg ccagcgccgc
      901 cgagaggatg tcgccggcac ccagaaaatc ggcatctatg cgttcgaagc tctggctgaa
      961 caactcggcc tcgggcgacg ccagcacgtt gcccagtcgg tcgccggcca gcggcagccc
     1021 ggtcaccagc acgaacgcca cgccgcgctc ggccgccgcc cgtgccaggt cggtggcgcg
     1081 cgcatggcgg tcggtggtgg attcgggcag cagccagtgg cgcagcgtgg cgttgttgcc
     1141 gaccagcacg gtggtctgcg gcagcagcag ctcgcggcac gcgtccaggt agtggtcgat
     1201 ttcggcttcg gaccaccacg acaggtcggg catgtaggcc accaccggca ccgacgcgta
     1261 gtccgaggtg aaggcggcaa tggccgacac cgcctcggca ctgcccacga aacccacctt
     1321 gatcacgtgc aacggcacgt cctcggccac ggcggccgcc tgttcggcca cggccgcctc
     1381 atcgatggcg cagtggctca tggtttcgcg cgtgtcgcgc acgtacacgc cggtgaccac
     1441 cggcagcgca tgggcgccga tggcggctat caccgtggca tcgctgcccg agccgccggc
     1501 accggtgggg tctccggcgt tgaagctcag gacgttgggc aaactgtcgt attcagccaa
     1561 caaatctggc gcgatcgcgc cgccggacgc atcggccggt gtggaactgc tcataggaca
     1621 cacattggcg ggccgactgt gacagagtgt cgtatgtagg cgaaagccgt cgatacaatc
     1681 gattgcattc taggtaattg caacttaagc tgtaatgaat cccaagactt ggatgtgcgt
     1741 gatctgcggt tggctttacg acgaagccgc gggagcacct gatcatggta tcgcgccagg
     1801 cacgccttgg gagcaggttc cggccgactg gtgctgccca gagtgcggcg cccacaagct
     1861 ggattttgaa atggtgcaga tctgagcctg cgaaatgcgt ggtctgtatg gacggcaaag
     1921 ccgggggcgt ccaggggcgt ccacgctaca accgagaggg tgcgatgaca tcaaggcggg
     1981 tgctggtcat tgacgacagc aacaccattc ggcgcagcgc cgaaatcttc ctcaagcagg
     2041 gagggcacga ggtcatgctg gccgaagacg gttttgacgc cttggccaag gtcaacgacg
     2101 cccagcctga cctgatcttc tgcgacatcc tgatgccgcg gctggatggc tatcagacct
     2161 gtgccatcat caaacgcaac gaaaagtatt catccatacc cgtggtcatg ttgtcgtcca
     2221 aggatggtgt gttcgacaag gcgcgcggcc gcatggtcgg ctcgcaggac tacctgacca
     2281 aacccttcac caaggatcaa ctgctgcagg cggtgcagca gttcggaaac gccaatcaag
     2341 gagccgtgtg atgtcgatcc gcaacgtgct gatcgtggac gattcgaaga ctgagttgct
     2401 gcacctgagc gaaatgctca agaaggcggg ctacgccgtg agcaccgcag aaaacgccga
     2461 ggaggccctg cgccgtctgg agcaggccaa gcccgatctg attctgatgg acgtcgtgat
     2521 gccgggccag aacggttttc agctcacgcg cgccatttcg cgcgatccgc gctacgccga
     2581 cgtgccgatc atcatgtgca ccagcaagaa ccaggaaacc gaccgggtct ggggcatgcg
     2641 ccagggtgcg cgcgattacg tcactaagcc ggtcgaccag gccgagctga tgggcaagat
     2701 cagagccttg ggctgatcga catgtccaac aagcaggcac tgcgcgaatt ccagagccgt
     2761 ctggcggcgc ggctgcagtc ggcccagacc gggggcgtgg cggcgtcgtg gctggcggtg
     2821 gaagccggat cgcagcgtct gctgtttccg ctcagtcatg cgggcgaaat cttctcctgg
     2881 accgacgtgc agcgcgtccc ttacgttcaa ccttggtttt tgggtgtggc caatctgcgc
     2941 ggtgggttgt cgggcgtgat cgatcttgcg cgtttcgtcg gcggcgcaga acctgtggcg
     3001 ggccgcagcg aggcggatct cgcgcaatgt cgtctggtgg cgctcaatcc ggtgcttggc
     3061 gccaactgcg cgttgctggt cgatcgcctg ttgggattgc gcaccaccga agcctttgcg
     3121 tcctcggagt cggcagaccc gatcgcgcct gcgtttttcg ggcaccggta cgccgatgcc
     3181 gatggtcagt cctggcagga gttgaacctg cagcttctct cgcagcaccc ggcctttctg
     3241 ggtatcgggg tgtaatttgt cttacggggt cacgacatgg cactcgcttc tcaactcacc
     3301 aatctgttcc gctccaagtc tcaggattcc tcaactggcg gtgacgaggc cgcggttcat
     3361 gaagagctgc tgagcacgcc gccaacgtcg catgctgacg attcgcgcgc cgcagcggag
     3421 agttcggtga tggtctcggc aatcgatgat tccgacgacg gcgctacgcc cggagacgat
     3481 gtgttttccg tccccctgct gggtacccgg acggcggcac agcatcagcg cctgctttcg
     3541 atactgctgg gactggcgtt gttcgccctg gtgatcgtgg gtttctggac ggtgtcgcgc
     3601 accgacaagg gtgcgcgcca ggtgggtgcg ataggtcagt cgctgatgca gtcgcaacgt
     3661 ttggccaaat cggtatcaca ggcgctggtc ggcagtcccg aggctttcgg tgaggtgaag
     3721 gaaagcgccg ccgccttgtc ggccaacgtg cgaggcctgc aggatggcag tgatgtggtg
     3781 cctgcgctga gcgacgcctt cagtgacgag ctgggcaaga tcgggccgct ggctcagcgt
     3841 gctgataaga gtgcggcggt cgtgatggcg cagcagcaga tcctgaccca ggtcggtgcc
     3901 gccctgcgcg cggtcagtcg acagtccacc gagttgctgg aagtggccga aaccgtgtcg
     3961 tcgctcaggt tgcagcagaa cgcatccgtc agtgagattt ccgcggcagg gcaactggtg
     4021 atgctgacgc agcgtatcgg caagtcggcc aatgaattcc tgaccatcga gggcgtgagc
     4081 ccagaggcgg tgttcctgct gggtaaggac ctcaatacct tcaaggagat cgccgagggg
     4141 ctgctcaacg gcagcgcaga cttgcgtctg gcgccggccc gtgatccgca gactcgcgaa
     4201 cgtctgacgg aactgctcaa gctttacgag gaaacccgca ctcaggccgg tggcgtgctg
     4261 ggcaacctgc agggtctggt cgcagcacgt gaagcgcaga ccaccatcgt gtccgacagc
     4321 gagccgttgc gcaaggcact ggaaggtctg caggacaagt tgtcgggcac ctcgggtgtc
     4381 ggattcggca acctgatgat gctggtcatt ccggcgattc tgctgttgct ggctgccgct
     4441 ggcctggttg ccgtgcaact gcgcgatagt cgcaagcgcc agacgctggc tgagcagcag
     4501 cgtcgttccg ccgagcagga tcagcaggaa gccaagcgcg tcaatgacgc caatcaggcg
     4561 gccatcttgc ggctcatgaa cgaactgcag acggtggctg aaggcgatct gacccaggaa
     4621 gccacggtga ccgaggacat caccggcgcc atcgccgact cggtgaact
//
LOCUS       DAOOOG010000192         4651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_22239_length_4651_cov_25.509138, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000192 DAOOOG010000000
VERSION     DAOOOG010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22239_length_4651_cov_25.509138"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PK242_11675"
     CDS             <1..770
                     /locus_tag="PK242_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ94721.1"
                     /translation="LKDKPIIDNYWQTETGWPILTICNGVERAPSKFGSPGKAVYGYD
                     IKLLDDQTGQELTAPNQKGVIAIEGPLPPGCMQTVWRDDARFVSTYWSSVPNRMVYST
                     FDWGIRDEDGYFFILGRTDDVINVAGHRLGTREIEEAIASHPNVAEVAVVGVADQLKG
                     QVAMAFAVLKDASILGDQAALLKLEGEVMKIVDGNLGAVARPARVRFVHVLPKTRSGK
                     VLRRAVQAVCEGRDPGDLTTMEDPAALQQIRDMVTPA"
     gene            950..3826
                     /locus_tag="PK242_11680"
     CDS             950..3826
                     /locus_tag="PK242_11680"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOZ94722.1"
                     /translation="MSETKSVYQTYQGNTYLFGGNAPYVEEMYENYLANPGSVPDSWR
                     SYFDALQHVPAADGSEARDVPHLPVINAFAERAKAGGTRVVVASADAELGRKRTAVQQ
                     LIAAYRNVGARWADLDPLKRAERPAIPELEPAFYGFTDADQETVFDTSNTFFGKDKMP
                     LRELLNALRETYCGTVGAEYMYATDQNQKRWWQSKLESIRTKPVFDVDKKKRILDRLT
                     AAEGLERYLHTKFVGQKRFSLEGGESFIAAMDELIQAAGAKGVQEIVIGMAHRGRLNV
                     LVNTLGKMPKELFAEFEHTAPEDLPAGDVKYHQGFSSDVSTAGGPVHLSLAFNPSHLE
                     IVNPVVEGSVRSRMDRRGDPKGKQVLPVLVHGDAAFAGQGVNQETLALSETRGYSTGG
                     TVHIIINNQIGFTTSDPRDLRSTLYCTDIVKMIESPVLHVNGDDPESVCLAMQLALEY
                     RMEFSKDVVVDIICYRKLGHNEQDTPMLTQPLMYKKIAQHPGTRKLYADKLAAQGLGQ
                     DLGDDMVRAYRAAMDAGKHTIDPVLTNFKSKYAVDWSPFLGKTWTDAADTAIPLTEWK
                     RLAERITTIPDSVTPHALVRKVYDDRAAMGRGETNVDWGMGEHMAFASLVASGYPVRL
                     SGEDSGRGTFTHRHAVIHDQKREKWDEGTYVPLQNVAEHQAPFTVIDSILSEEAVLAY
                     EYGYASNDPNTLVIWEAQFGDFANGAQVVIDQFIASGEVKWGRVNGITLMLPHGYEGQ
                     GPEHSSARLERFMQLAADANMQIVQPTTASQIFHVLRRQMVRNLRKPLVIFTPKSLLR
                     NKDATSPLSEFTKGGFQTVLGEQDAAIAKNAAKVKRVIACSGKVYYDLVKKRSEAERS
                     DVAIVRVEQLYPFPHKAFAAELKKYPNATDIVWCQDEPQNQGAWFFIQHNIHENMHEG
                     QKLGYAGRAASASPAVGYAHLHQDQQKALIDAAFGRLKGFVLQK"
     gene            3928..>4651
                     /locus_tag="PK242_11685"
     CDS             3928..>4651
                     /locus_tag="PK242_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HOZ94723.1"
                     /translation="MAIVEVKVPQLSESVAEATLLQWKKKAGEAVQVDEILIEVETDK
                     VVLEVPAPASGVLTEIVQADGATVVADQLLARIDTAAVAGAAAPAPAAAPSAAPAPAA
                     APAADKSGVAMPAAAKLMADHQLPAGSVAGTGKDGRVTKGDVLGAIQSGAAGAGGASA
                     GAQKAAQIPTGVPTTALPQVAAPAAPQLGDRPEQRVPMTRLRARVAERLLQSQASNAI
                     LTTFNEVNMQPVMDMRKRFQDKF"
BASE COUNT          881 a         1613 c         1500 g          657 t
ORIGIN      
        1 cgctcaagga caagccgatc atcgacaact actggcagac cgagaccggc tggccgatcc
       61 tgaccatctg caacggcgtc gagcgggcgc ccagcaagtt cggctcaccg ggcaaggcgg
      121 tgtacggcta cgacatcaag ctgctggacg accagaccgg ccaggagctg acggcgccca
      181 accagaaggg tgtgatcgcc atcgaaggcc cgctgccgcc cggctgcatg cagaccgtct
      241 ggcgcgacga cgcgcgcttc gtcagcacct actggtccag tgtgcccaac cgcatggtgt
      301 attccacctt cgactggggc atccgcgacg aagacggcta tttcttcatc ctcgggcgca
      361 ccgacgacgt catcaacgtc gccggccacc gcctgggcac gcgcgagatc gaagaagcca
      421 tcgccagcca tcccaacgtc gccgaagtgg cggtggtggg cgtggccgac cagctcaagg
      481 gccaggtcgc catggccttc gcggtgctca aggacgccag catcctgggc gaccaagcgg
      541 cgctgctgaa gctggagggc gaggtgatga agatcgtcga cggcaacctg ggcgcggtgg
      601 cgcgcccggc gcgcgtgcgc ttcgtccacg tgctgcccaa gacgcgctcg ggcaaggtgc
      661 tgcgccgcgc cgtgcaggcg gtgtgcgagg ggcgcgatcc gggcgatctg accaccatgg
      721 aagacccggc ggcgctgcag cagatccggg acatggtcac gccggcgtga gtggcgcgcc
      781 ggcgcggcgg gccgtcacat gcccgtcagt gttcggtggc acaatccgca gcttgcgtag
      841 tacacggccc ttcccagcct gtcgcgtccg ccaaccggtt cagccgtgac gcggaaggtt
      901 ttccaaccaa ctgaagctcc ctgaaggggg gcgaaaggtc agcgaagcaa tgagcgagac
      961 gaaatccgtc tatcaaacct accagggcaa cacctatctc ttcggcggca atgcgcccta
     1021 cgtcgaagag atgtacgaaa actacctggc caacccgggc agcgtgccgg actcctggcg
     1081 cagctacttc gatgccctgc agcacgtgcc ggcggccgac ggcagcgagg cgcgtgacgt
     1141 gccgcacctg ccggtcatca acgcctttgc cgaacgcgcc aaggccggtg gcacgcgggt
     1201 ggtggtggcc agtgccgacg ccgaactggg acgcaagcgc accgcggtgc agcagttgat
     1261 cgccgcctac cgcaacgtgg gcgcccgctg ggccgacctc gaccccctga agcgcgccga
     1321 gcgccccgcc atccccgagc tcgaaccggc cttctacggc ttcaccgacg ccgatcagga
     1381 aacggtgttc gacaccagca acaccttctt cggcaaggac aagatgccgc tgcgcgagct
     1441 gctcaatgct ctgcgcgaga cctactgcgg caccgtgggc gccgaataca tgtacgccac
     1501 cgaccagaac cagaagcgct ggtggcagag caagctggag tcgattcgca ccaagccggt
     1561 gtttgacgtc gacaagaaaa agcgcatcct cgatcgcctg acggccgccg agggtctgga
     1621 gcgctacctg cacaccaagt tcgtcggtca gaagcgcttt tcgctggagg gcggcgaatc
     1681 cttcattgcc gccatggacg agctgatcca ggccgccggc gccaagggcg tgcaggaaat
     1741 cgttatcggc atggcccacc gtggccgcct gaacgtgctg gtcaacaccc tgggcaagat
     1801 gcccaaggag ctgttcgccg agttcgagca caccgcgccc gaagacctgc ctgccggcga
     1861 cgtgaaatac caccagggct tcagctccga cgtgtccacc gccggtggcc cggtgcacct
     1921 gtcgctggcc ttcaacccct cgcacctgga aatcgtcaac ccggtggtcg aaggctcggt
     1981 gcgttcgcgc atggaccgtc gcggcgaccc caagggcaag caggtgctgc cggtgctggt
     2041 gcacggtgac gccgccttcg ccggccaggg cgtgaaccag gaaacgctgg cgctgtccga
     2101 gacccgtggc tattccacgg gcggtacggt gcacatcatc atcaacaacc agatcggctt
     2161 caccaccagc gacccgcgcg acctgcgctc cacgctgtac tgcaccgaca tcgtcaagat
     2221 gatcgagtcg ccggtgctgc acgtcaacgg cgacgacccc gagtcggtct gcctggccat
     2281 gcagctggcg ctggaatacc gcatggagtt ttccaaggac gtggtggtgg acatcatctg
     2341 ctaccgcaag ctgggccaca acgagcagga cacccccatg ctcacccagc cgctgatgta
     2401 caaaaagatc gcccagcacc ccggcacgcg caagctctac gccgacaagc tggccgccca
     2461 gggtctggga caggacctgg gcgacgacat ggtgcgcgcc taccgcgccg ccatggacgc
     2521 cggcaagcac accatcgatc cggtgctgac caacttcaag agcaagtacg cggtcgactg
     2581 gtcgcccttc ctgggcaaga cctggaccga cgccgccgat accgccattc cgctgaccga
     2641 gtggaagcgc ctggccgagc gcatcaccac catccccgac agcgttacgc cgcacgcgct
     2701 ggtcaggaag gtctacgacg accgcgccgc catgggccgg ggcgagacga acgtggactg
     2761 gggcatgggc gaacacatgg cgtttgcctc cctggtggcc agcggctacc ccgtgcgcct
     2821 gtccggcgag gacagcggcc gaggcacctt cacgcaccgc cacgccgtga tccacgacca
     2881 gaagcgcgag aagtgggacg aaggcaccta cgtgccgctg cagaacgtgg ccgagcacca
     2941 ggcgccgttc accgtcatcg actccatcct gtccgaagag gccgtgctgg cctacgaata
     3001 cggttacgcc tccaacgacc ccaacacgct ggtgatctgg gaggcgcagt tcggcgactt
     3061 cgccaacggc gcgcaggtgg tgatcgacca gttcatcgcc tcgggtgagg tcaagtgggg
     3121 ccgtgtgaac ggcatcacgc tgatgctgcc gcacggctac gagggccagg gccccgagca
     3181 cagctcggca cgcctggagc gcttcatgca gctggcggcc gacgccaaca tgcagatcgt
     3241 gcagccgacc acggcgagcc agatcttcca cgtgctgcgc cgccagatgg tgcgcaatct
     3301 gcgcaagccc ctggtcatct tcacgcccaa gagcctgctg cgcaacaagg acgcgacctc
     3361 gccgctgtcc gagttcacca agggcggttt ccagaccgtg ctgggcgagc aggatgccgc
     3421 gattgccaag aacgcggcca aggtcaagcg cgtcatcgcc tgctcgggca aggtgtacta
     3481 cgacctcgtg aagaagcgca gcgaggccga gcgcagcgac gtggccatcg tccgcgtcga
     3541 gcagctctac ccgttcccgc acaaggcctt cgcggccgag ctcaagaagt acccgaacgc
     3601 caccgacatc gtgtggtgcc aggacgagcc gcagaaccag ggcgcctggt tcttcatcca
     3661 gcacaacatc cacgagaaca tgcacgaagg tcagaagctg ggctacgccg gccgggccgc
     3721 ctcggcatca cccgccgtgg gttacgcgca cctgcaccag gaccagcaga aggcgctgat
     3781 cgatgccgcc ttcggccgtc tgaaaggctt cgtgctgcag aagtaaggat ggaggttgcc
     3841 gggcccgggc tgtagcgctt ccgccagctt ccgccctcgc gccggcgcca gtcgcaaccg
     3901 cattcaagaa caccggaaga atcccaaatg gctatcgtcg aagtcaaagt tccccaactg
     3961 tccgaatccg tggccgaggc cacgctgctg caatggaaga agaaggccgg tgaggccgtc
     4021 caggtcgatg aaattctgat cgaggtcgaa accgacaagg tggtgctgga agtgcccgcg
     4081 cccgccagcg gcgtgctgac cgagatcgtt caggccgacg gcgccaccgt ggtggccgac
     4141 cagctgctgg cgcgcatcga caccgccgcc gtggccgggg ccgccgcgcc cgccccggcc
     4201 gccgcgccga gcgccgctcc tgcgcccgcc gccgcccccg cggccgacaa gtccggcgtg
     4261 gccatgcccg ccgccgccaa gctgatggcc gaccaccagc tgcccgccgg cagcgtggcc
     4321 ggcaccggca aggacggtcg ggtgaccaag ggcgatgtcc tgggcgctat tcaatcagga
     4381 gctgctggcg caggcggggc aagcgctgga gcccaaaaag ctgctcagat cccgaccggt
     4441 gtgccaacca ccgcgctgcc ccaggtggcg gccccggcgg cgccccagtt gggcgaccgc
     4501 cccgagcagc gcgtgcccat gacgcgcctg cgcgcgcgcg tggccgagcg cctgctgcag
     4561 tcgcaggcca gcaacgccat cctgaccacg ttcaatgaag tgaacatgca gccggtgatg
     4621 gacatgcgca agcgcttcca ggacaagttc g
//
LOCUS       DAOOOG010000193         4647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_22267_length_4647_cov_28.198824, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000193 DAOOOG010000000
VERSION     DAOOOG010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22267_length_4647_cov_28.198824"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /gene="argH"
                     /locus_tag="PK242_11690"
     CDS             complement(<1..886)
                     /gene="argH"
                     /locus_tag="PK242_11690"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOZ94724.1"
                     /translation="MSANQLDKKSQAWSALFSEPMSELVQRYTASVDFDRRLASADIT
                     GSLAHADMLAAQGIISAQDLADIRRGMAQIQGEIERGEFSWQLALEDVHLNIEARLTQ
                     LVGDAGKRLHTGRSRNDQVATDIRLWLRGEIDLITGLLRELQGALIEVAARHIDVILP
                     GFTHLQVAQPVSFAHHLLAYVEMFSRDEQRMLDARKRVNQLPLGSAALAGTTYPLDRE
                     RVARTLGMDGVCQNSLDAVSDRDFAIEFSAAASLVMVHISRLSEELILWMSQNFAFIR
                     IADRFTTGSSIMPQKKNPD"
     gene            997..2049
                     /locus_tag="PK242_11695"
     CDS             997..2049
                     /locus_tag="PK242_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ94725.1"
                     /translation="MQDSQILSTFGERKRPPKARPAPPPLVFDACQVGVILRAVLYVE
                     AVIGVGLMYLTADLSDWLARVALVTGGALPATLLWLVLACALKRLLARLSWPLQAAAG
                     VALGALAGLYACGTLALLGFAAAPPWLASASTGALLAAGLMAGLLWRVRGRQPAATTA
                     RLAELQSRIRPHFLFNTLNTAIALVRAEPAKAERVLEDLSDLFRHALAEQGDSATVGQ
                     EIELAQRYLEIEQLRFGERLRVEWALDERVHGARLPPLILQPLVENAVKHGVEPSPGG
                     AELRISTQRRGQRALIKVTNTVPAGQGRAGFGIAQANVRDRLRLLHDLEGDFRTALVD
                     GVYQARIEVPLPGRGA"
     gene            2049..2795
                     /locus_tag="PK242_11700"
     CDS             2049..2795
                     /locus_tag="PK242_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ94726.1"
                     /translation="MALQVLVVDDEPLARARLRALLADCREPEAVAAAEAGTAAQALA
                     LAQHQRFDVALLDIHMPGADGMQLACALRALPEPPRVVFVTAHAEHAVRAFEIDAVDY
                     LTKPVRLERLQAALQKTQRVAQLQSAPEPDSAPDWLLITERHRTLRVPLAEVLYLKAE
                     LKYITVRTADASHLLDGSLNQLEERHGGRFLRIHRSTLVARNAIRALVRHQDPDEGEG
                     WAVQLAGLDEALPVSRRQLAAVRAALGEPR"
     gene            2891..3796
                     /gene="hemC"
                     /locus_tag="PK242_11705"
     CDS             2891..3796
                     /gene="hemC"
                     /locus_tag="PK242_11705"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOZ94727.1"
                     /translation="MSLSITIATRESRLALWQAEHVRALLTQRGHSVRLLGMTTQGDQ
                     ILDRTLSKVGGKGLFVKELETALEDGRADIAVHSLKDVPMELPAGLQLACVMAREDPR
                     DAWVSPAHASLDELPAGAVVGTSSLRRTVLLRALRPDLRIEPLRGNLDTRLRKLDEGR
                     YAGIVLAAAGLKRLGLAARIRHIFDSDAMLPAAGQGALGIEIREGRGELTQALAPLAD
                     QPTWLAVAAERAVSRAMGGSCSMPLAAHATRQGERLHLRAAWGDPEGAPVLVRAEGEA
                     QVSTLAQAEALGERVAAGLRAGGAR"
     gene            3805..4596
                     /locus_tag="PK242_11710"
     CDS             3805..4596
                     /locus_tag="PK242_11710"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOZ94728.1"
                     /translation="MAAPRVLVTRPAHDAAHWVGQLRQCGIDAQALPLIDIVPEPLAG
                     RLLQARQHLGDYAVAMFVSGNAVTGFMGSNQAAAQLQQALKAIETRAWSPGPGTTAAL
                     QRAGWPAARIDQPAPEAPQFDSESLWAQVAPQVRPGLRVLIVRGGDAAGSVAGRDWLA
                     AQLTAAGAEVDQVLAYRRTAPRLDAAGLARATAAARDGTLWLFSSSEAIAHLRQCLPQ
                     IGWQAARALVTHPRIGAAARAAGFGTVHHSQPTLDAVSASIKSLA"
BASE COUNT          607 a         1650 c         1742 g          648 t
ORIGIN      
        1 cgtcggggtt tttcttctgc ggcatgatcg acgagccggt ggtgaagcgg tcggcgatgc
       61 ggatgaaggc gaagttctgg ctcatccaca gaatcagctc ttcgctcaga cggctgatgt
      121 gcaccatcac caggctggcg gcggcggaaa attcgatcgc gaagtcgcgg tcgctcaccg
      181 cgtccaggct gttctggcag acgccatcca tgcccagcgt gcgcgccacg cgctcgcggt
      241 ccagcgggta ggtggtgccg gccagggcgg cgctgcccag cggcagctgg ttgacgcgct
      301 ttcgggcatc cagcatgcgc tgttcgtcgc ggctgaacat ctcgacgtag gccagcaggt
      361 ggtgggcaaa gctcacgggc tgggccacct gcaggtgggt gaagccgggc aggatgacgt
      421 cgatgtgcct ggcggccacc tcgatcagcg cgccctgcag ctcgcgcagc aggccggtga
      481 tcaggtcgat ctcgccgcgc agccacaggc ggatgtcggt ggccacctgg tcgttgcggc
      541 tgcggccggt gtgcaggcgc ttgccggcgt cgcccaccag ctgggtcagg cgcgcctcga
      601 tgttcaggtg cacgtcttcc agcgccagct gccagctgaa ttcgccgcgc tcgatctcgc
      661 cctggatctg cgccatgccg cgcctgatgt cggccaggtc ctgggcgctg atgatgccct
      721 gcgccgccag catgtcggcg tgcgccaggc tgccggtgat gtcggcgctg gccaggcgcc
      781 ggtcgaagtc gacgctggcc gtgtagcgct gcaccagctc gctcatcggt tcagagaaca
      841 gggcggacca ggcctgggat ttcttgtcga gttggttggc ggacatgcgg gtgtgaagaa
      901 gtggcgcgcg gactgacgaa cggcgcggct ggacggacgg acgcgcccgg agcgcgcggg
      961 ttgcggccta cactgcggcc acaatcgcca cggcccatgc aggactcgca aattctatcg
     1021 accttcggtg aacgcaagcg gccgcccaag gcgcgcccgg cgccgccacc gctggtgttc
     1081 gacgcctgtc aggtgggggt gatcctgcgc gccgtgctgt acgtggaggc ggtgatcggg
     1141 gtcgggctga tgtacctcac cgccgacctg tcggactggc tggcgcgggt ggcgctggtt
     1201 accggcggcg ccctgccggc cacgctgctg tggctggtgc tggcctgcgc gctcaagcgc
     1261 ctgctggcgc gcctgagctg gccgctgcag gccgccgccg gggtggcgct gggggcgctg
     1321 gccggcttgt acgcctgcgg cacgctggcg ctgctgggtt ttgccgccgc gccgccctgg
     1381 ctggccagcg ccagcaccgg ggcgttgctg gccgccggcc tgatggccgg gttgctgtgg
     1441 cgcgtgcgcg gtcgccagcc ggcggccacc acggcgcggc tggccgagct gcagtcgcgc
     1501 atccgcccgc attttttgtt caacaccctc aacaccgcca tcgccctggt gcgcgccgag
     1561 ccggccaagg ccgaacgggt gctggaagac ctgtccgacc tgttccgcca cgcgctggcc
     1621 gagcagggcg acagcgccac cgtcggccag gaaatcgagc tggcgcagcg ctatctggag
     1681 atcgagcaac tgcgtttcgg cgagcgcctg cgggtggagt gggcgctgga cgagcgcgtg
     1741 cacggcgcgc gcctgccgcc gctgatcctg cagccgctgg tggaaaacgc cgtcaagcac
     1801 ggcgtcgagc ccagccccgg cggcgccgag ctgcgcattt cgacccagcg gcgcggccag
     1861 cgcgcgctga tcaaggtcac caacaccgtg ccggccgggc aggggcgggc gggcttcggc
     1921 atcgcgcagg ccaacgtgcg cgaccggctg cgcctgctgc acgatctgga gggcgacttc
     1981 cgcacggcgc tggtggacgg cgtatatcag gcccgcatcg aggtgccctt gccggggcga
     2041 ggggcgtgat ggcgctgcag gtgctggtgg tcgacgacga gccgctggcg cgtgcgcgcc
     2101 tgcgcgcgct gctggccgac tgccgcgagc ccgaggctgt ggccgccgcc gaggccggca
     2161 cggccgccca ggcgctggcc ctggcgcagc accagcgctt cgatgtggcg ctgctggaca
     2221 tccacatgcc gggggccgac ggcatgcagc tggcctgcgc gctgcgcgcc ttgcccgagc
     2281 cgccgcgggt ggtgttcgtc accgcccacg ccgaacacgc ggtgcgcgcc ttcgagatcg
     2341 atgccgtcga ctacctgacc aagccggtgc ggctcgaacg cctgcaggca gcgctacaaa
     2401 aaacgcagcg cgtggcgcag ttgcagagcg cgccagagcc cgattcggcc cctgactggt
     2461 tgctcatcac cgagcgccac cgcacgctgc gcgtgccgct ggcggaggtg ctctacctga
     2521 aggccgagct caagtacatc accgtgcgca cggccgacgc cagccatctg ctggacggca
     2581 gcctgaacca gctggaagag cgccacggcg ggcgctttct gcgcatccac cgcagcacgc
     2641 tggtggcgcg caacgccatc cgggccctgg tgcgccacca ggacccggac gagggcgagg
     2701 gctgggccgt gcaactggcc ggcctcgacg aagcgctgcc ggtgtcgcgc cggcagctgg
     2761 cggcggtgcg tgcggcgctc ggcgagccgc gctgaacggc gcgccggccg tcgccggcgg
     2821 ccgggcgcac agggcacgcc gctgaggtct cgcgggtgcc gggccgcggc gtccggtagc
     2881 atcgcccccc atgtctcttt ccatcaccat cgccacccgt gaaagccgcc tggccctgtg
     2941 gcaggccgaa catgtgcgtg ccctgctgac gcagcgcggc cacagcgtgc ggctgctggg
     3001 catgaccacc cagggcgacc agatcctcga ccgcacgctc agcaaggtgg gcggcaaggg
     3061 cctgttcgtc aaggagctgg aaaccgcgct cgaagacggc cgcgccgaca ttgccgtgca
     3121 ctcgctcaag gacgtgccga tggagctgcc cgcgggcctg cagctggcct gcgtgatggc
     3181 gcgcgaggac ccgcgcgacg cctgggtgtc gcccgcgcac gccagcctgg atgagctgcc
     3241 ggccggcgcc gtggtcggca cctccagcct gcgccgcacg gtgctgctgc gcgccctgcg
     3301 gcccgacctg cgcatcgagc cgctgcgcgg caacctcgac acgcggctgc gcaagctcga
     3361 cgaaggccgg tacgccggca tcgtgctggc cgccgccggc ctcaagcgcc tggggctggc
     3421 ggcgcgcatc cgccacatct tcgacagcga cgccatgctg ccggcggccg ggcagggggc
     3481 gctgggcatc gaaatccgcg aaggccgcgg cgagctgacg caggcgctgg cgccgctggc
     3541 cgaccagccg acctggctgg cggtggcggc cgaacgcgcc gtcagccgcg ccatgggggg
     3601 cagctgctcg atgccgctgg cggcgcacgc cacgcggcag ggcgagcgtc tgcacctgcg
     3661 cgctgcctgg ggcgaccctg aaggcgcgcc ggtgctggtc agggccgagg gcgaggccca
     3721 ggtaagcacc ctggcgcagg ccgaagccct gggcgagcgt gtggcggccg ggctgcgcgc
     3781 cggcggcgcc cgttgagccg gcccatggct gcgccgcgcg tgctcgtcac ccggccggcg
     3841 cacgacgccg cccactgggt gggccagctg cgccaatgcg gcatcgacgc gcaggcgctg
     3901 ccgctgatcg acatcgtgcc cgagcccctg gccggtcgcc tgctgcaggc gcgccagcac
     3961 ctgggcgact atgccgtggc gatgttcgtc agcggcaacg cggtcaccgg gtttatgggg
     4021 tcaaatcagg ctgcagcgca gttgcagcaa gcgctgaaag ctatcgaaac aagagcatgg
     4081 tcgcccggtc cgggcaccac ggcggcgctg cagcgggccg gctggccggc cgctcgcatc
     4141 gatcagccgg cgcccgaggc gccgcaattc gactccgaat cgctgtgggc ccaggtggca
     4201 ccgcaggtgc gccctggcct gcgcgtgctg atcgtgcgcg gcggtgacgc cgcgggcagc
     4261 gtggccggcc gcgactggct ggcggcgcag ctgacggcgg ccggcgccga ggtcgatcag
     4321 gtgctggcct accgccgcac ggcgccgcgg ctggatgccg ccggcctggc gcgggccacg
     4381 gcggcggcgc gcgacggcac gctgtggctg ttcagcagct ccgaagccat tgcccacctg
     4441 cgccagtgcc tgccgcagat cggctggcag gcggcgcgcg cgctggtcac ccacccacgc
     4501 atcggcgccg cggcgcgggc cgccgggttt ggcacggtgc accacagcca gcccacgctg
     4561 gacgcggtga gtgcgtcgat aaaatcgctc gcatgacgtc cgagccgagc cccgcagcgc
     4621 ctgccgcgcc gccccctgtg ccggcgc
//
LOCUS       DAOOOG010000194         4621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_22438_length_4621_cov_28.875821, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000194 DAOOOG010000000
VERSION     DAOOOG010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22438_length_4621_cov_28.875821"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /gene="sufB"
                     /locus_tag="PK242_11715"
     CDS             complement(<1..1018)
                     /gene="sufB"
                     /locus_tag="PK242_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOZ94729.1"
                     /translation="MASPEPISTAQALADKPQEAPLEQALQRPYQHGFVTDIESDSLP
                     PGLDEGTIRAISRKKKEPQFLLDWRLAAFRRWQTMALPDWARLKIAPIDFQALSYYSA
                     PKSMKDKPKSLDEVDPKLLETYEKLGVPLHERAALAGVAVDAVFDSVSVGTTFRKQLA
                     DVGVIFCSFSHAVQNHPELVEKYLGTVVPQGDNFYAALNSAVFSDGSFVYIPKGVKCP
                     MELSSYFRINAQNTGQFERTLIIAEEGSSVSYLEGCTAPQRDENQLHAAVVELIAHDD
                     AFIKYSTVQNWYPGDEHGVGGIYNFVTKRGLAAGKRSRIAWTQIETGSAITWKYPSCV
                     LRGEG"
     gene            1164..2441
                     /locus_tag="PK242_11720"
     CDS             1164..2441
                     /locus_tag="PK242_11720"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HOZ94730.1"
                     /translation="MQIYMVGGAVRDRLLGLPVQDHDWVVVGATPEDMVARGFVPVGR
                     DFPVFLHPSSHEEYALARTERKSGRGYKGFTVFTSPEVRIEDDLARRDLTINAIAVRA
                     GQASAGAKFDHEDGVIDPFGGRRDLQARVLRHVTDAFREDPVRILRVARFAARFHDFS
                     VAPETRALMRQMVAEGEADHLVPERVWQELARGLMESQPARMFEVLRDCGALAVVLPE
                     LDRLWGVPQTASHHPEIDTGVHVMLVLQMAARLQAPLAVRWACLMHDLGKGETPADLL
                     PRHLGHEQRSARLLRAVGQRLRVPTDCTELAEVVAREHGHIHRSAELGAAALLRLLQR
                     CDALRKPERFAQVLLACECDARGRLGFEHSAYPQRARLQAALGAAQSVATAGLAQAAQ
                     AAGARGADIGQAVAQARTRALQAWLDGSAPAAV"
     gene            2567..3190
                     /locus_tag="PK242_11725"
     CDS             2567..3190
                     /locus_tag="PK242_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ94731.1"
                     /translation="MARPISAAHGLRRDEILDVAADCFARQSYPATSMNDIAAACGTS
                     KARLYHYYDSKDAILFELLDRYTQRLLVIIGQVQATAHRQALDERATLHALIRAFLRE
                     YESSATRHAALLASTQFLGETQRGVILDRQRDVVSAVTRFLRRAYPERVDARNQTAIT
                     MTLFGMINWTFTWLRPGGSLSYDAFADEVIALLERGLSSPLNPHSRS"
     gene            3187..4620
                     /gene="glmU"
                     /locus_tag="PK242_11730"
     CDS             3187..4620
                     /gene="glmU"
                     /locus_tag="PK242_11730"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOZ94732.1"
                     /translation="MNPIDIAVMAAGKGTRMKSRTPKVLQKLAGRPLLGHVLDTAAQL
                     RARSVVVITGHGATEVEAFGANLAGAGAGFDLQFARQEPQLGTGHAMQQAMPRLADDG
                     LVVVLSGDVPLTEAATLQALVDAAQGERLALLSVTLADPSGYGRIVRGAGGAVQRIVE
                     HKDASAAERAIGEIYSGIMAVPARLLRGWLTRLTNHNAQGEYYLTDVVAMAVADGVPV
                     VAHCIQDALQVAGVNSPLQLAELERAHQLRQARALMEAGVRLADPARFDLRDDARRGV
                     RAELRCGPDVDIDVGCIFTGRVEIGEGAQIGAHCCIANARIEAGAVIHPYTHIDGEAA
                     GVHVGPGALVGPFARLRPGADLGPEVHIGNFVEVKNSSLAAGAKANHLAYLGDAVVGE
                     RVNYGAGSITANYDGARKHRTVIEADVHVGSNCVLVAPVTIGAGGTVGGGSTITRDTP
                     PGGLSVARGRQAHIANWQRPAKPQGGG"
BASE COUNT          679 a         1604 c         1643 g          695 t
ORIGIN      
        1 aaccctcgcc gcgcagcacg caggaggggt acttccaggt gatggccgag ccggtctcga
       61 tctgcgtcca ggcgatgcgc gagcgcttgc cggccgccag gccgcgcttg gtgacgaagt
      121 tgtagatgcc gccgacgccg tgctcgtcgc ccgggtacca gttctgcacc gtggagtact
      181 tgatgaaggc gtcgtcgtgg gcgatcagct cgaccaccgc ggcgtgcagc tggttttcgt
      241 cgcgctgcgg cgcggtgcag ccttccaggt agctgaccga gctgccctct tcggcaatga
      301 tgagggtgcg ctcgaactga ccggtgttct gggcgttgat gcgaaagtac gacgacagct
      361 ccatcgggca cttgacgccc ttggggatgt agacaaacga gccgtcggaa aacaccgccg
      421 agttgagcgc ggcgtagaag ttgtcgccct gcggcaccac cgtgcccagg tatttttcga
      481 ccagctcggg gtggttctgc accgcgtgcg agaacgaaca gaaaatcacg cccacgtcgg
      541 ccagctgctt gcggaaggtg gtgcccaccg agaccgagtc gaacaccgca tccaccgcca
      601 cgccggccag cgccgcgcgc tcgtgcagcg gcacgccgag cttttcgtag gtctcgagca
      661 gcttggggtc gacctcgtcg aggctcttgg gcttgtcctt catggacttg ggcgccgagt
      721 agtaggacag ggcctggaag tcgatcggcg cgatcttcag gcgcgcccag tcgggcagcg
      781 ccatggtctg ccagcgccgg aacgcggcca ggcgccagtc gagcaggaac tgcggctcct
      841 tcttcttgcg cgagatggcg cggatggtgc cctcgtccag gccgggcggc aggctgtcgg
      901 actcgatgtc ggtcacgaag ccgtgctggt agggccgctg cagcgcctgc tcgagcggcg
      961 cttcctgcgg cttgtccgcc agggcctgcg cggtgctgat gggttcaggt gatgccatgg
     1021 gcgctcatcc gtttaaatgt acatatcgta tgcatctttg gccgactcgg caaaccggct
     1081 gcgccgtgcc tgcacctgag atggggacat tgtccgggca gggcggcgcg gccggggcgc
     1141 cgcactgctc ctacacttgc cccatgcaga tctacatggt gggtggcgcc gtgcgggacc
     1201 gcttgctcgg cctgccggtg caggatcacg actgggtggt ggtgggcgcc acgcccgaag
     1261 acatggtggc ccgcggcttc gtgccggtag ggcgggattt tcccgttttt ctgcacccct
     1321 cgtcgcatga ggaatatgcg ctggcgcgca ccgagcgcaa gagcggccgt ggctacaagg
     1381 gtttcaccgt cttcaccagc cccgaggtgc gcatcgaaga cgacctggcg cgccgcgacc
     1441 ttactatcaa tgcaatagct gttcgcgcag gccaggcaag cgctggagcc aaatttgacc
     1501 atgaagacgg ggtgatcgat ccgtttggcg gccggcgcga cctgcaggcg cgtgtgctgc
     1561 gccacgtcac cgacgccttc cgtgaagacc cggtgcgcat cctgcgcgtg gcgcgttttg
     1621 cggcgcgctt tcacgacttc agcgtggcgc ccgagacgcg ggcgctgatg cgccagatgg
     1681 tggccgaggg cgaagccgac cacctggtgc ccgagcgcgt gtggcaggag ctggcgcgcg
     1741 gcctgatgga aagccagccg gcgcgcatgt tcgaggtgct gcgcgactgt ggcgcgctgg
     1801 ccgtggtgct gcccgagctc gaccgcctgt ggggcgtgcc gcagaccgcc tcgcaccacc
     1861 ccgagatcga caccggcgtg cacgtgatgc tggtgctgca gatggccgcg cgcctgcagg
     1921 cgccgctggc ggtgcgctgg gcctgcctga tgcacgacct gggcaagggc gaaacaccgg
     1981 ccgacctgct gccgcgccac cttggccacg agcagcgcag cgcccgcctg ctgcgcgccg
     2041 tcggccagcg cctgcgcgtg cccaccgact gcaccgagct ggccgaggtg gtggcgcgcg
     2101 agcacggcca catccaccgc agcgccgagc tgggcgccgc cgccctgctg cggctgctgc
     2161 agcgctgcga cgcgctgcgc aagcccgagc gctttgccca ggtgctgctg gcctgcgaat
     2221 gcgacgcgcg cgggcggctg ggcttcgagc acagcgcgta cccgcagcgt gcgcgcctgc
     2281 aggcggcgct gggcgcggcg cagtcggtgg cgacggcggg gctggcgcag gcggcgcagg
     2341 ccgccggcgc gcgcggcgcc gacatcggcc aggcggtggc gcaggcgcgc acacgggcgc
     2401 tgcaggcctg gctggacggg tcggccccgg ccgcggtctg atcggcgccg ccgattgcta
     2461 cgaattggat agctgttggc gcagtctggg taagcgccag gacttgtttt ttctgtgaac
     2521 ggcgggcgcg ccggcggcgc cggccgggcg ccgataatca cgccccatgg cccgcccgat
     2581 ctccgccgcc cacggtctgc gccgcgacga aatcctcgac gtcgcggccg actgctttgc
     2641 gcgccagagc tacccggcca ccagcatgaa cgacatcgcc gccgcctgcg gcacctccaa
     2701 ggcgcggctg taccactatt acgacagcaa ggacgccatc ctttttgagc tgctggatcg
     2761 ctacacccag cggctgctgg tcatcatcgg ccaggtgcag gccacggcac accgccaggc
     2821 gctggacgag cgcgccaccc tgcacgcgct gatccgcgcc tttctgcgcg aatacgaaag
     2881 ttctgccacg cgtcatgcgg cgctgctggc cagcacccag ttcctgggcg aaacccagcg
     2941 cggcgtgatc ctggatcgcc agcgcgacgt ggtgtcggcc gtcacgcgct ttctgcgccg
     3001 cgcctacccc gagcgggtgg acgcgcgcaa ccagaccgcc atcaccatga cgctgttcgg
     3061 catgatcaac tggaccttca cctggctgcg ccccggcggg tcgctgagct acgacgcctt
     3121 cgccgacgag gtgattgcgc tgctggagcg cgggctgtct tcacccctga accctcactc
     3181 acgttcatga acccgatcga tatcgccgtc atggctgccg gcaagggcac gcgcatgaaa
     3241 agccgcaccc ccaaggtgct gcagaaactg gccggccggc cgctgctggg ccatgtgctg
     3301 gacaccgccg cccaactgcg ggcgcgctcg gtggtcgtca tcaccggcca cggtgctact
     3361 gaagttgaag cgtttggcgc aaacctggcg ggcgccggag ccggatttga cttgcaattc
     3421 gcacgtcagg agccgcagct gggcaccggc cacgccatgc agcaggccat gccgcggctg
     3481 gccgacgatg gtctggtggt ggtgctgtcg ggcgacgtgc cgctgaccga agcggccacc
     3541 ctgcaggccc tggtcgacgc cgcccagggc gagcggctgg cgctgctcag cgtgacgctg
     3601 gccgacccca gcggctacgg ccgcatcgtg cgcggcgccg gcggcgccgt gcaacgcatc
     3661 gtcgaacaca aggacgccag cgcggccgag cgcgccatcg gcgagatcta cagcggcatc
     3721 atggcggtgc cggcgcgcct gctgcgcggc tggctgacac ggctgaccaa ccacaacgcc
     3781 cagggcgagt actacctgac cgacgtggtg gccatggccg tggccgacgg cgtgccggtg
     3841 gtggcgcact gcatccagga cgcgttgcag gtggccggcg tcaacagccc gctgcaactg
     3901 gccgagctgg agcgcgccca ccagctgcgc caggcgcggg cgctgatgga agccggcgtg
     3961 cgcctggccg acccggcgcg cttcgacctg cgcgacgacg cccgccgcgg cgtgcgcgcc
     4021 gaactgcgct gcgggccgga cgtggacatc gacgtcggct gcatcttcac cgggcgggtc
     4081 gagatcggcg agggcgcgca gatcggcgcc cactgctgca tcgccaacgc ccgcatcgag
     4141 gcgggggcgg tgatccaccc ttacacccac atcgacggcg aagccgccgg cgtgcacgtc
     4201 ggcccgggcg ccctggtggg gccgttcgcc cgcctgcgcc ccggcgccga cctggggccc
     4261 gaggtgcaca tcggcaactt cgtcgaagtc aagaactcca gcctggccgc cggcgccaag
     4321 gccaaccacc tggcctatct gggcgacgcc gtggtgggcg agcgcgtcaa ctacggcgcc
     4381 ggcagcatca ccgccaacta cgacggcgcc cgcaagcacc gcacggtgat cgaggccgac
     4441 gtgcatgtgg gcagcaactg cgtgctggtg gcgccggtga ccatcggcgc cggcggcacc
     4501 gtgggcggcg gctccaccat cacgcgcgac accccgccgg gcgggctgag cgtggcgcgc
     4561 gggcgccagg cccacatcgc caactggcag cgcccggcca agccgcaagg tggcggctga
     4621 g
//
LOCUS       DAOOOG010000195         4505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_23226_length_4505_cov_27.712584, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000195 DAOOOG010000000
VERSION     DAOOOG010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23226_length_4505_cov_27.712584"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PK242_11735"
     CDS             complement(<1..740)
                     /locus_tag="PK242_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOZ94733.1"
                     /translation="MPRPSQLLHPQREPARPLPAATVLLLRDTPAGIEVLMTRRSLNA
                     SFAPGAYVFPGGGIDPQDAAAHGQATCRPTQSQAHLTQAIAAIRESFEELGVLLARRA
                     DGTPADASDVAALDRHAPLAAQCAARGLTLAAADVHMLAHWITDRDLPRRFDVPFLVA
                     RMPDGQTPVADDAEQFEPVWVRPADALARHEAGQFFIIFPTIRTLQRLQHYPDVAAVL
                     AACASERPLWASCPRAGWLAGKEARYME"
     gene            846..1580
                     /gene="ubiE"
                     /locus_tag="PK242_11740"
     CDS             846..1580
                     /gene="ubiE"
                     /locus_tag="PK242_11740"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOZ94734.1"
                     /translation="MATTHFGFQSVDEQDKARRVRGVFDSVAARYDLMNDLMSGGLHR
                     AWKAYAVMVANLQPGQSVLDIAGGTGDLALAFARRVGPHGQVVHTDINEAMLRTGRDR
                     LLDAGVILPTTVCDAEALPFPDERFDVVSVAFGLRNMTHKDQALREMARVLKPGGRLL
                     VLEFSQVARPLRKAYDLYSFQVLPRLGRLVAGDADSYRYLAESIRMHPNQEELKALMK
                     ANGFGHVDYHSMTAGVVALHVGIKCG"
     gene            1642..2646
                     /locus_tag="PK242_11745"
     CDS             1642..2646
                     /locus_tag="PK242_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HOZ94735.1"
                     /translation="MNLKKLVSHFFTLLLAVVLTGAFSFEAEAKRLGGGKSLGRQSSN
                     VTQREAAGPGTPVAPAQSSATAGAAAGGAAAAGAAGAAARKPWGSMLGGLAAGLGLAW
                     LAHALGLGQAFGNVLLILLLGMVVLAVIGMLRRGRGAAAAGGPGMAYQGAGAAQPITP
                     RQYNPTKVGNDASARPWEGQAAPFDAGGASGGSIIGSGLRGAQGWGVPADFDIAGFTE
                     AAKRNFITLQDAWDRSDIPALRAMMTDEMLAEIQSQLAERERGAPGQANQTEVVMLEA
                     QLLGIEELADVYMASVEFSGMIREDASAGPSPFREVWNMTKPRQGRGGWLVAGVQALQ
                     "
     gene            2779..3321
                     /locus_tag="PK242_11750"
     CDS             2779..3321
                     /locus_tag="PK242_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94736.1"
                     /translation="MTMKSPFSLVAEAFGKLGEQLKAPDWLVDEMQHRVVLFLNHVLM
                     QEPEAQARLARQQGRVVRVQWRDFNLQLAATPAGLCERVPEATPDLLLTVTDSSPLDL
                     ARHAVRGERPGVRIEGDVQFAAEVNWLVDHVRWDLEEDLSRLIGDAPAHQLARLGSTL
                     AQGLRRFIASLPGQDRAGGA"
     gene            3341..>4505
                     /locus_tag="PK242_11755"
     CDS             3341..>4505
                     /locus_tag="PK242_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOZ94737.1"
                     /translation="MTRVLRATTIVWVALRHGLDELVLTSFEHPGLRLLARIATLGRD
                     LSAPRGQRLREALQRLGPIFVKFGQVLSTRRDLLPPDIADELALLQDRVPPFPPEAAV
                     ATIERAFRKPLGELFTHFERQPVASASIAQVHFATLRDAGGREREVAVKVLRPGMLKV
                     IEQDLQLIRMMATWVERLSADGKRLKPREVVAEFDKYLHDELDLMREAASAAQLRRNM
                     AGLNLVLIPEMIWELARPDVLVMERMQGIPISQVERLREAGVDIPRLARDGVTIFFTQ
                     VFRDGFFHADMHPGNIMVSVDPATFGRYISLDFGIVGTLTEVDKEYLAQNFTAFFRRD
                     YKRVAELHIESGWVPAGTRVDELESAVRSVCEPYFDRPLKDISLGMVLMRLFQT"
BASE COUNT          645 a         1513 c         1656 g          691 t
ORIGIN      
        1 tgctccatgt agcgcgcttc cttgccggcc agccagccgg cgcgcgggca gctggcccat
       61 agcggccgtt cgctggcgca ggcggccagc acggcggcga cgtcggggta gtgctgcagg
      121 cgctgcagcg tgcggatggt ggggaagatg atgaagaatt gccccgcctc gtggcgcgcc
      181 agcgcgtcgg ccgggcgcac ccacacgggt tcgaactgct cggcgtcgtc ggccaccggg
      241 gtctggccgt ccggcatgcg ggcgaccaga aagggcacgt cgaagcgccg cggcaggtcg
      301 cggtcggtga tccagtgcgc cagcatgtgg acgtcggccg ccgccagcgt caggccgcgc
      361 gcggcgcact gggccgccag cggggcgtgg cggtcgagcg ccgccacgtc gctcgcgtcg
      421 gccggcgtgc cgtcggcacg ccgcgccagc agcaccccca actcctcgaa gctttcgcgg
      481 atggcggcga tggcctgggt caggtgggcc tggctctgcg tgggtcggca tgtcgcctga
      541 ccgtgggcgg cggcgtcctg ggggtcgatg ccgccgccgg gaaacacgta ggcgccgggg
      601 gcaaaactgg cgttcagcga gcggcgcgtc atcagcactt cgatgccggc gggcgtgtcg
      661 cgcagcagca gcacggtggc ggcgggcagg ggacgcgcgg gttcgcgctg cggatgcaga
      721 agctggctgg ggcgtggcat gcggccatta tgggcatggc gcgccttaca cttgacgcca
      781 gggcgtcagc cgcgcgagcg gtgggcgccg tttcccgcag cgatccacca ccacggcagc
      841 gccccatggc caccacgcat ttcggtttcc agtcggttga cgaacaggac aaggcgcgcc
      901 gcgtgcgcgg cgtgttcgat tcggtcgccg cgcggtacga cctcatgaac gacctcatgt
      961 ccggtggcct gcaccgggcc tggaaggcct atgccgtgat ggtggccaac ctgcagccgg
     1021 gtcagagcgt gctggacatc gccggcggca ccggcgatct ggcgctggcc ttcgcccgcc
     1081 gggtcggccc gcatgggcag gtggtgcaca ccgacatcaa cgaagccatg ctgcgcaccg
     1141 ggcgcgaccg cctgctggat gccggggtga tcctgcccac cacggtgtgc gacgccgagg
     1201 ccttgccttt tcccgacgag cggttcgacg tggtcagcgt cgccttcggt ctgcgcaaca
     1261 tgacgcacaa ggaccaggcc ctgcgcgaga tggcgcgcgt gctcaagccg ggcggtcggc
     1321 tgctggtgct ggagttttcg caggtggcgc ggccgctgcg caaggcctac gacctgtatt
     1381 cgttccaggt gctgccgcgg ctggggcggc tggtggcagg cgacgccgac agctaccgct
     1441 acctggccga atcgatccgc atgcacccca accaggaaga actcaaggcg ttgatgaagg
     1501 ccaacggttt cggtcatgtc gactatcaca gcatgacggc tggggtcgtt gccttgcatg
     1561 tcggcatcaa atgcggataa cctttataaa gccgtggata tccgtgtgga actttcagtc
     1621 gtcaggggag tccggtcagt catgaacctc aagaagctcg tttctcattt cttcacgctg
     1681 ctgctggcgg tggtgctgac cggcgcgttc agcttcgagg ccgaggccaa gcgcctgggc
     1741 ggtggcaagt cgctcgggcg ccagtccagc aacgtgacgc agcgcgaagc ggccggcccc
     1801 ggcacgccgg tggcgccggc gcagtccagc gccaccgccg gtgccgccgc gggcggtgcg
     1861 gcggcggcag gcgcggccgg tgcggccgcg cgcaaaccct ggggcagcat gctgggcggt
     1921 ctggcggccg gtctgggcct ggcctggctg gcccacgcgc tgggcctggg tcaggcgttc
     1981 ggcaacgtcc tgctgatcct gttgctgggc atggtggtgc tggcggtgat cggcatgctg
     2041 cgccgcgggc gtggtgccgc ggccgccggc gggcccggca tggcctacca gggcgccggt
     2101 gccgcgcagc cgatcacacc gcgccagtac aacccgacca aggtgggcaa cgacgcctcg
     2161 gcccgtccct gggaaggtca ggcggcgccg tttgacgccg gtggcgcctc ggggggatcg
     2221 atcatcggct ccgggctgcg tggcgcccag ggctggggtg tgccggccga tttcgacatc
     2281 gccggcttca ccgaggccgc caagcgcaac ttcatcaccc tgcaggatgc ctgggaccgc
     2341 tccgacatcc cggcgctgcg cgccatgatg accgacgaga tgctggccga aatccagtcc
     2401 cagctggccg agcgcgagcg cggtgcgccg ggtcaggcca accagaccga ggtggtgatg
     2461 ctggaggcgc agttgctggg catcgaggag ctggccgacg tgtacatggc cagcgtcgag
     2521 ttcagcggca tgatccgcga ggacgcctcg gccggcccga gcccgttccg cgaggtctgg
     2581 aacatgacca agccgcgcca gggccgcggc ggctggctgg tggcgggggt gcaggcactg
     2641 cagtgacgac cggccgggcg taccggcccg gcccgccccg aagcccggct tgccgggctt
     2701 ttttgtgtcc gtgccaggca ggaagaacgc gtgcatctgg gcggccgacg ccgccatttg
     2761 tcaggaataa tcgcccccat gacgatgaag tcccccttct cactggtggc cgaggcattc
     2821 ggcaagttgg gcgagcagct gaaggcgccc gactggctgg tcgacgagat gcagcaccgc
     2881 gtggtgctgt tcctcaacca cgtgctgatg caggagcccg aagcccaggc ccggctggcg
     2941 cgccagcagg gtcgcgtggt gcgcgtgcag tggcgcgact tcaacctgca gctggcggcc
     3001 accccggccg gactgtgcga gcgcgtgccc gaggccacgc ccgacctgct gctcacggtc
     3061 accgacagct cgccgctcga tctggcgcgc cacgccgtgc gcggcgagcg gcccggggtg
     3121 cgcatcgagg gcgatgtgca gttcgccgcc gaagtcaact ggctggtcga ccacgtgcgc
     3181 tgggatctgg aggaagacct gtcgcggctg atcggcgacg ctcccgcgca ccagctggca
     3241 cgcctgggca gcaccctggc gcaggggctg cgcaggttca tcgccagcct gcccggtcag
     3301 gaccgggcag gcggcgcatg agcgcgtcgg ggaggcgccg gtgacgcgcg tgctgcgggc
     3361 caccaccatc gtgtgggtgg ctctgcgcca tgggctggac gaactggtgc tgaccagctt
     3421 cgagcacccg ggcctgcgcc tgctggcgcg catcgccacc ctcgggcgcg atctgtcggc
     3481 gccgcggggc cagcgcctgc gtgaggcgct gcagcgcctg gggccgatct tcgtcaagtt
     3541 cggccaggtg ctgtcgacgc ggcgcgacct gctgccgccc gacattgccg acgaactggc
     3601 cctgctgcag gaccgcgtgc caccgtttcc gcccgaagcg gcggtggcca ccatcgagcg
     3661 tgccttccgc aagccgctgg gcgagctgtt cacgcatttc gagcgccagc cggtggccag
     3721 cgcctccatc gcccaggtgc actttgccac cctgcgcgac gccggcgggc gcgagcgcga
     3781 ggtggcggtg aaggtgctgc gcccgggcat gctgaaggtg atcgagcagg atctgcagct
     3841 gatccgcatg atggccacct gggtcgaacg cctgtcggcc gatggcaaac gcctcaagcc
     3901 gcgcgaggtg gtggccgaat tcgacaaata cctgcacgac gagctggacc tgatgcgcga
     3961 agccgccagc gccgcgcagc tgcggcgcaa catggcgggg ctgaacctgg tgctgatccc
     4021 ggagatgatc tgggagctgg cgcggcccga cgtgctggtg atggagcgca tgcagggcat
     4081 tccgatcagc caggtcgagc gcctgcgcga ggccggggtc gacatcccca gactggcgcg
     4141 cgacggcgtg accatcttct tcacccaggt gtttcgcgac ggcttcttcc acgccgacat
     4201 gcacccgggc aacatcatgg tcagcgtcga tccggccacc ttcgggcgct acatctcgct
     4261 cgatttcggc atcgtcggca cgctgaccga ggtcgacaag gaatacctgg cgcaaaactt
     4321 caccgccttc ttccgccgcg actacaagcg cgtggccgag ctgcacatcg aaagcggctg
     4381 ggtgccggcc ggcacgcgcg tggacgagct cgaatcggcc gtgcgcagcg tgtgcgagcc
     4441 ctacttcgat cggccgctga aggacatctc gctcggcatg gtgctgatgc gcctgttcca
     4501 gacct
//
LOCUS       DAOOOG010000196         4454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_23568_length_4454_cov_26.167538, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000196 DAOOOG010000000
VERSION     DAOOOG010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23568_length_4454_cov_26.167538"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="PK242_11760"
     CDS             complement(<1..1323)
                     /locus_tag="PK242_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HOZ94738.1"
                     /translation="MPWIALQWPPEAPPALCPAAEAAPAAPGQGQPGTPAEALGWWAL
                     QFTPRVARVDEALLLEVSGTLRLWGGLPALMHRIEAQNPAPPLIWQAPAAIGLIALAM
                     LRLRATGQALPPPAALPEALPLDVLSAARPHLPLLVRLGCRTWGQAHALPRAGLVRRC
                     GPELRAALDVAFALAPESYPWLTAPERFDQALDLHTRVDNAPALLWAAARLLTALQHW
                     LRARQLGVLALELAWTLDGKRLDGRPLPPVQSLRVRTAQPAQAMEHLRRLLAERLARV
                     SLWAPAVRLRLQALETAPWQAGNHSLLPEERRAGDPLHVFIERVSARLGPQQVLMAEP
                     LADHRPEQRQRWRPAQQASAVAVPGWADALAPTWLLPEPLPLAEQDGRPFHHGPLHLL
                     AGPHRIEAGWWPDGGDRSAQAPERDQASQPIAGPLPRRAAAPLGGSAAS"
     gene            complement(1292..2080)
                     /gene="imuA"
                     /locus_tag="PK242_11765"
     CDS             complement(1292..2080)
                     /gene="imuA"
                     /locus_tag="PK242_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HOZ94739.1"
                     /translation="MPTPAGVWRADALDAGAAPAFTTGHAALDAALPGGGWPLGALVE
                     LLPATDDGGVPVWPLLLPALAAHRVGQGAGTVVLVGAPASPRPAADWQPGLPALAAAG
                     LSPARLLWLPGASPVARLWAAEQALRCADVAAVLAWLPQARASDLRRLQLAAARRGDG
                     LLFVLRPAQAARTASPAPLRLRLGVDVVASTLVVHVVKRRGPPLVAPLHLPAQSAAVR
                     ALLAAAEQARAAAAARVLPFAPRPDRADAAREVDHAVDRVAVAA"
     gene            2456..3511
                     /gene="lipA"
                     /locus_tag="PK242_11770"
     CDS             2456..3511
                     /gene="lipA"
                     /locus_tag="PK242_11770"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOZ94740.1"
                     /translation="MSDSPRPQRPVSGSKVITIHGTRAVLDGVKPSALSEVPDAGPKP
                     PWLRVRLPTGAKYQDVMEIVQSHRLSTVCAESKCPNIAECWGRGTATLMLMGSVCTRA
                     CKFCSVNTGNPKGWLDPQEPANVANAVALMDLRYVVLTSVDRDDLPDLGAGHYAACIR
                     AIHDRMPDTAVEALTPDFQGRHELVAQVLDAGLATYAQNLETVKRLTHPVRDPRAGYE
                     LTLDVLKFAKRHAPGTITKTSLMVGLGETDDEIEQALDDMREAGVDVVTLGQYMRPTR
                     NHLPVQRFVTPEQFQRYRDMALAKGFLEAVSGPLVRSSYRAERVLDQDNVGLGPQAVL
                     DAIRESAAAAATPSLRC"
     gene            3616..3951
                     /locus_tag="PK242_11775"
     CDS             3616..3951
                     /locus_tag="PK242_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94741.1"
                     /translation="MVPCIVVCHFQLPVPTEAQFIDIARRSAPMFRQLGERGLISKDY
                     VRGEGGAGGVYVWESRAAAEAWFTEAKLAEYTQIFGARPTLTWYDAHLTVDNKAGQVR
                     INGQPVAGS"
     gene            4009..>4454
                     /gene="thyA"
                     /locus_tag="PK242_11780"
     CDS             4009..>4454
                     /gene="thyA"
                     /locus_tag="PK242_11780"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOZ94742.1"
                     /translation="MTAEPRAPSCDQYETFMRHVYEHGVAKADRTGTGTRSVFGHQMR
                     FDLAAGFPLVTTKKVHLKSIIYELLWFLRGDGNARWLQERGVTIWDEWANPDTGDLGP
                     VYGVQWRSWPTPDGGHIDQIAQVVDQLKTNPDSRRIIVSSWNVAELP"
BASE COUNT          658 a         1719 c         1573 g          504 t
ORIGIN      
        1 gcttgctgcg ctgcccccca agggggccgc cgcccgcctt gggagcggcc cggcgatggg
       61 ttgcgacgcc tgatcgcgct ccggtgcctg agcggaccgg tcgccgccat cgggccacca
      121 gccggcttcg atgcggtgcg gcccggccag caggtgcagc gggccgtggt ggaacggccg
      181 gccgtcctgc tcggccagcg gcagcggctc gggcagcagc caggtgggcg ccagcgcgtc
      241 ggcccagccg ggcacggcca cggctgacgc ctgctgcgcc ggtcgccagc gctggcgctg
      301 ctcgggccga tggtcggcca acggctcggc catcagcacc tgctgtggtc ccagacgggc
      361 gctgacgcgc tcgatgaaca cgtgcagcgg atcgccggcg cgccgctcct cgggcagcag
      421 gctgtggttg ccggcctgcc agggcgcggt ctccagcgcc tgcaggcgca gccgcacggc
      481 cggcgcccac aaggacacgc gcgccagccg ctcggccagc aggcggcgca ggtgctccat
      541 ggcctgcgcc ggctgggcgg tgcgcacccg cagcgactgc acgggcggca gcggccggcc
      601 gtcgaggcgc ttgccgtcca gcgtccatgc cagctccagc gccagcaccc cgagctggcg
      661 cgcgcgcagc cagtgctgca gcgccgtgag caggcgcgcg gcggcccaca gcagcgcggg
      721 ggcgttgtcg acccgggtgt gcaaatcgag cgcctggtcg aagcgctcgg gcgccgtcag
      781 ccaggggtag ctttcgggcg ccagagcgaa ggccacgtcc agcgcggcac gcagctcggg
      841 cccgcagcgg cgcaccagcc cggcgcgcgg cagcgcgtgc gcctggcccc aggtgcggca
      901 gcccaggcgc accagcagcg gcaggtgcgg gcgggcggcg ctcagcacgt ccagcggcag
      961 cgcctcgggc agcgccgccg gcggcggcag cgcctgcccg gtggcgcgca ggcgcagcat
     1021 cgccaatgct attaaaccaa tagctgctgg cgcttgccag ataagcggtg gagccggatt
     1081 ttgtgcctcg atacggtgca tcagcgcggg caacccgccc cacagccgca gggtgccgga
     1141 gacttccagc agcagcgctt catcgacccg cgccacgcgc ggggtgaatt gcagcgccca
     1201 ccaccccagc gcctcggcgg gcgtgccggg ctgcccctgt ccgggcgcgg ccggggcggc
     1261 ctccgcggcg ggacagaggg ccggcggcgc ctcaggcggc cactgcaacg cgatccacgg
     1321 catggtccac ctcgcgtgcg gcatcggctc gatcggggcg cggcgcgaac ggcagcaccc
     1381 gggcggcggc cgccgcccgg gcctgctcgg ccgccgccag cagcgcccgc accgccgcgg
     1441 actgcgccgg caggtgcagc ggcgcgacca gcggcggccc gcgccgcttg acgacgtgca
     1501 ccaccagggt gctggccacc acgtccacgc ccagccgcag gcgcagtggc gcgggcgagg
     1561 cggtgcgcgc cgcctgcgcc ggtcgcagca cgaacagcag gccatcgccg cgcctggccg
     1621 ccgccagttg cagccgccgc agatcgctgg cccgcgcctg cggcagccag gccagcacgg
     1681 ccgccacgtc ggcgcagcgc agcgcctgct cggccgccca caggcgcgcg acgggcgacg
     1741 cgcccggcag ccacagcagc cgcgccggcg acaggccggc cgccgccagt gccggcaggc
     1801 cgggctgcca gtcggcggcc ggccgtggcg acgccggcgc ccccaccagc accaccgtgc
     1861 ccgcgccctg cccgacccgg tgcgccgcca gcgccggcag cagcaggggc cagaccggca
     1921 cgccgccgtc gtcggtcgcc ggcagcagct cgaccaacgc gcccagcggc cagcccccgc
     1981 ccggcagcgc ggcatccagc gcggcgtggc ccgtggtgaa ggccggcgct gcgccagcgt
     2041 ccagggcgtc ggcgcgccac accccggcgg gcgtcggcac cgcaggctgc ggcctgtctg
     2101 gcggcgccga aacggcacgc gcgcccaccc cgtgagcgcg ccacgcgcgg gcggtcaggg
     2161 acgaatcaag ggccatggca gacatcgaaa actgtttatt tatacagtta ttttgagccc
     2221 ttcccggccg ccctggcaag cgctttcggg cccgtcgcgg caggccttgc ggagcatggt
     2281 ggcggcgctc ccgggtttgc agcacggggg cgatccgggc gtggcgggcc ggtctggccg
     2341 gggggtggcg gcgccaatcc cctggcaggc ggcggtcgac cccaccctca tagaatcgag
     2401 cctgtgcgcc cgcccgctgg caggcgcggc atttcacacg cgagaccgag gctccatgag
     2461 cgattccccc cgcccccaac gtccggtgtc cggcagcaag gtcatcacca tccacggcac
     2521 gcgcgccgtg ctggacggcg tcaagcccag cgccctgagc gaagtgcccg acgccgggcc
     2581 caagccgccc tggctgcgcg tgcgcctgcc caccggcgcc aagtaccagg acgtgatgga
     2641 gatcgtgcaa tcgcaccggc tgtcgaccgt gtgcgccgaa tccaagtgcc ccaacatcgc
     2701 cgaatgctgg ggccgcggca ccgccaccct gatgctgatg ggctcggtgt gcacgcgcgc
     2761 ctgcaagttc tgctcggtca acaccggcaa ccccaagggc tggctggacc cgcaggagcc
     2821 ggccaacgtg gccaacgccg tggcgctgat ggatctgcgc tacgtggtgc tgacctcggt
     2881 cgaccgcgac gacctgcccg acctgggtgc cggccactac gcggcctgca tccgcgccat
     2941 ccacgaccgc atgcccgaca ccgcggtgga ggcgctgacg cccgacttcc agggccggca
     3001 cgaactggtg gcgcaggtgc tggacgccgg cctggccacc tacgcgcaga acctggaaac
     3061 cgtcaagcgc ctgacccacc cggtgcgcga cccgcgcgcc ggctacgagc tgacgctgga
     3121 cgtgctgaag ttcgccaagc gccacgcgcc gggcaccatc accaagacca gcctgatggt
     3181 ggggctgggc gaaaccgacg acgaaatcga gcaggcgctg gacgacatgc gcgaagccgg
     3241 cgtcgacgtg gtcaccctgg gccagtacat gcggccgacc cgcaaccacc tgccggtgca
     3301 gcgcttcgtc acgcccgagc agttccagcg ctaccgcgac atggccctgg ccaagggctt
     3361 tctggaagcg gtcagcggcc cgctggtgcg ctccagctac cgcgccgagc gcgtgctgga
     3421 ccaggacaac gtcggcctgg gcccgcaggc ggtgctggac gccatccgcg aatcggcggc
     3481 ggccgccgcc acaccgtcgc tgcgctgctg acggcgccgc ggccacacag ggccgcgccc
     3541 caccccgccc ccggccggca gggacgcccc aggcccctgc ctacaatcgt ttgccctccc
     3601 gaggagatcc acgccatggt gccttgcatc gtcgtctgcc acttccagct tccggtaccc
     3661 accgaagcgc aattcatcga catcgccagg cgctcggcgc cgatgttccg ccagctgggc
     3721 gaacgcggcc tgatcagcaa ggactacgtg cgcggcgaag gcggcgccgg cggcgtctac
     3781 gtgtgggaaa gccgcgccgc cgccgaagcc tggttcaccg aagccaaact tgccgaatac
     3841 acccagatct tcggcgcgcg gcccacgctg acctggtacg acgcgcacct gacggtggac
     3901 aacaaggcgg gccaggtgcg catcaatggc cagcccgtgg ccggcagctg aaacgcggcg
     3961 ccccgcccag cacccgcacc ccgaagccac ccacctgcag ccgatgtcat gaccgctgaa
     4021 ccgcgcgcac cttcctgcga ccagtacgaa accttcatgc gccacgtgta cgagcacggc
     4081 gtggccaagg ccgaccgcac cggcaccggc acgcgcagcg tgttcggcca ccagatgcgc
     4141 ttcgacctcg cggcgggctt tccgctggtc accaccaaga aggtacacct gaaaagcatc
     4201 atttatgaat tgctgtggtt cctgcgcggc gacggcaacg cccgctggct gcaagagcgc
     4261 ggcgtgacga tctgggacga atgggccaac cccgacaccg gcgacctggg cccggtctac
     4321 ggcgtgcagt ggcgcagctg gcccacaccc gacggtggcc acatcgacca gatcgcgcag
     4381 gtcgtcgacc agctcaagac caaccccgac agccgccgca tcatcgtcag cagctggaac
     4441 gtggccgagc tgcc
//
LOCUS       DAOOOG010000197         4408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_23898_length_4408_cov_22.575465, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000197 DAOOOG010000000
VERSION     DAOOOG010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23898_length_4408_cov_22.575465"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="PK242_11785"
     CDS             <1..1151
                     /locus_tag="PK242_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOZ94743.1"
                     /translation="GRRMQTISGIHYNWSLPGLDNDGHFALIRNFRRHAFLLLYLFGA
                     SPAVCASFVAGRAHELQPLGAHAMHLPWATSLRMGRLGYQSDAQATLAVSYNGLESYA
                     TTLEEALTRPYPAYEAVGIRNPGGDYNQLATTLLQIENEFYGTIRPKRVIRTGERPLH
                     ALRERGVEYLEVRLMDLNPFEPIGIDAATARFLDLFLLHCLLTDSPPDTPQEIAALKA
                     NQHLTAARGREPGLCLDRRGESVRLSDWAAQLVGELAPIADAMDAVHGGRAHAQALVQ
                     ARQRLAAPGELPSARVLQAVRDQHGSSFVAFTRQQALATRAHLLALDYPAAIKQRFDA
                     LAVESVQAQADIEAADRVPFETFRREYLSSRHLRPAASSSAMPGSSGA"
     gene            1594..>4408
                     /locus_tag="PK242_11790"
     CDS             1594..>4408
                     /locus_tag="PK242_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94744.1"
                     /translation="MITAAEFIAKWGPGGSAAQLNEEQGAQSHFLDLCQLLGVPRPGD
                     DVGDEYLFERQSLVLGQARGYADVFYRNHFAWENKAPGKNLDAALRQLQQYSLALSNP
                     PLLVVCDRLTLRIHTQFNGHPSETHTVRIDQLDQPDKQQLLRRLWLDPGSFRPRQTNR
                     DITEAAARSFATLAEQLRRRGHHADAVAHFLTQCLFCFFAEDVGLLPGRMFERLVGNR
                     QLTSDKLSAGLQSLFATMQTGGLYGPDDIPWFNGGLFAHIAVPKLEIIDITELRNAAS
                     LNWSAIDVSIFGTLFERGLDPAKRSQLGAHYTDPATIERIIGPVITRPLLQQWELVAQ
                     DLQALAAKIKRKGDAAYHKAHQRFVQWLEQLRAFRVLDPACGSGNFLFLGLKALKDIE
                     LHTITEAARLGLDREQDLVTGPHNLLGIELNEYAAELARVSVWIGEIQWRIAHGYAPK
                     TNPVLEPLEQIECRDALLTFSPPLPPPGEGREGGQPAAAPGATASGPHPNPPPAGEGA
                     PAHDAKAAASAPLPPLGEGREGGQPAAAPGATASGPHPNPPPAGEGATAAPREADWPR
                     ADVVVGNPPFLGDKWMRSELGDAYTDALRATYAGQVPGGADFVTYWFHKAGQAMRTQT
                     LQRAGLVATNSIRQRKNRQVLAQASAQAPIFAAWSDLPWVNEGAAVRVSLVCWGAPSD
                     TPALLDGAPVQSIHPDLTGQAIGTVSFDLTQAQPLPENAQASYFGLCLAGPFKVPAAT
                     AAEWLRKPNVHGRPNSDVLKPIFNGRDVTDRWSGQWVVDFGARMSEQDAALYEAPFQY
                     VQQHVLPVRASNKRAARAKYWWRHGEARPGLRDKWEGLSHYIATVETAKHRVFVRMPM
                     AHAPEHSLIVIPRSDDVTFGVLSSRMHVLWAVARGGRMGLGNDPRYNSLAAFAPFPFP
                     PGLTPQDTAHQRTEALASGALIPA"
BASE COUNT          729 a         1669 c         1389 g          621 t
ORIGIN      
        1 acggccggcg catgcagacc atctcgggca tccactacaa ctggtcgctg cccggcttgg
       61 acaacgacgg ccacttcgcg ctgatccgca acttccgccg ccacgccttc ctgctgctgt
      121 acctgttcgg cgcctcgccg gcggtgtgcg ccagcttcgt ggccggccgc gcgcacgagc
      181 tgcagccgct gggcgcgcac gccatgcacc tgccctgggc gacttcgctg cgcatggggc
      241 ggctgggcta ccagagcgat gcccaggcca cgctggcggt cagctacaac ggcctggaaa
      301 gctacgccac caccctggaa gaggcgctga cacggcccta cccggcctac gaagcggtcg
      361 gcatccgcaa cccgggcggc gactacaacc agctggccac cacgctgctg cagatcgaaa
      421 acgagttcta cggcaccatc cgccccaagc gcgtgatccg caccggcgag cgcccgctgc
      481 atgcgctgcg tgagcgcggc gtcgaatacc tcgaagtgcg cctgatggac ctgaacccgt
      541 tcgagccgat cggcatcgac gcggcgacgg cgcgctttct cgaccttttc ctgctgcact
      601 gcctgctcac cgacagcccg cccgacacac cgcaggaaat cgccgcgctc aaggccaacc
      661 agcacctgac cgccgcgcgc gggcgcgagc ccggcctgtg cctggatcgc cgtggcgagt
      721 cggtgcggct gagcgactgg gccgcgcagc tcgtcggcga gctggcaccc atcgccgacg
      781 ccatggacgc cgtgcatggt ggccgtgcgc acgcccaggc actggtgcag gcccgccagc
      841 gcctggcggc gcctggcgag ctgccctcgg cgcgcgtgct gcaggcggtg cgcgaccagc
      901 acggcagcag tttcgtggcc ttcacacggc agcaggcgct cgccacccgc gcgcacctgc
      961 tggcgctgga ctacccggct gccatcaagc agcgctttga cgcgctggcg gtcgagtcgg
     1021 tgcaggccca ggccgacatc gaagcggccg accgcgtgcc gttcgaaacc ttccggcgcg
     1081 aatacctgtc gtcacggcat ctgcggcccg ccgcaagctc gtccgccatg ccgggcagct
     1141 ccggcgcctg accgctgccg gccccccatc ctcgagacgt ccggtcccaa tccggttgcc
     1201 ggccgtgacg ggcacgcgtg cctgtcgtgt ccaggccggc gccgccgtcg ggcccaagtg
     1261 cagtgtttcg cgccttcggg tgcttatagc gagtcgccaa ggggccagct gcgcggcgcg
     1321 tggccgcagg catggtggag ccatgtcaag gccacgccac aaagcagatg gtcccttggc
     1381 ggcgcgccct gcggggagcc ggaaaacggc ttgcggcagc gttgcgcctg ttgccaaggc
     1441 cgccagcatg ggctgtgagc cgcgccttgc cgcaagcctg tttctggctt ccataagtgc
     1501 ctcacagcgc gaaacactgc agtaggaaca aaacagagct ccagcgcaca accatcaagc
     1561 gccagcagct catgatttga tagtatctcg cccgtgatca ccgccgccga attcattgcc
     1621 aagtggggcc ctggcggcag cgccgcgcag ctcaacgaag agcagggcgc gcaaagccac
     1681 tttctcgacc tgtgccagct gctgggcgtg ccccggccgg gcgacgacgt gggcgacgaa
     1741 tacctgttcg agcgccagtc gctggtgctg ggccaggcgc gcggctacgc cgacgtcttc
     1801 taccgcaacc acttcgcctg ggaaaacaaa gcccccggca aaaacctcga cgccgcgctg
     1861 cgccagctgc agcaatacag cctggcgctg tccaacccgc cgctgctggt ggtgtgcgac
     1921 cgcctcaccc tgcgcatcca cacccagttc aacggccacc ccagcgagac gcacaccgtc
     1981 cgcatcgacc agctcgacca gcccgacaag cagcaactgc tgcgccgcct gtggctcgat
     2041 cccggcagct ttcgtccgcg ccagaccaac cgcgacatca ccgaagccgc cgcccgcagc
     2101 tttgccaccc tggccgagca actgcgccgc cgcggccacc acgccgacgc cgtggcgcac
     2161 ttcctcacgc aatgcctgtt ctgcttcttc gccgaagacg tgggcctgct gcccggccgc
     2221 atgttcgagc gcctggtcgg caaccgccag ctcaccagcg acaaactcag cgccggcctg
     2281 caaagcctgt ttgccaccat gcagaccggc ggcctgtacg ggcccgacga catcccctgg
     2341 ttcaacggcg ggctgtttgc gcacatcgcc gtgcccaagc tggaaatcat cgacatcacc
     2401 gaactgcgca acgccgccag cctgaactgg agcgccatcg acgtcagcat cttcggcacc
     2461 ctgttcgagc gcgggctcga ccccgccaag cgcagccagc tcggcgccca ctacaccgac
     2521 ccggccacca tcgaacgcat catcggcccc gtcatcaccc gcccgttgct acaacaatgg
     2581 gagctggtgg cgcaagacct gcaagcgctg gcggccaaaa tcaagcgcaa gggcgacgcc
     2641 gcctaccaca aggcccacca gcgcttcgtg cagtggctcg aacagctgcg cgccttccgc
     2701 gtgctcgacc ccgcctgcgg cagcggcaac ttcctgtttc tgggcctgaa ggcgctcaag
     2761 gacatcgagc tgcacaccat caccgaagcc gcccgcctgg ggctggaccg cgagcaggac
     2821 ctggtcaccg gcccgcacaa cctgctgggc atcgagctca acgaatacgc cgccgagctg
     2881 gcccgcgtca gcgtctggat cggcgaaatc cagtggcgca tcgcccacgg ctacgcgccc
     2941 aagaccaacc ccgtgctgga gccgctggag cagatcgaat gccgcgacgc gctgctgacc
     3001 ttctccccac cccttccccc gccgggggaa ggcagggaag ggggccagcc cgcagccgcg
     3061 ccaggggcca ccgccagcgg cccccacccc aaccctcccc cagcggggga gggagccccc
     3121 gcgcacgacg ccaaggccgc cgcctctgcg ccccttcccc cgctggggga aggcagggaa
     3181 gggggccagc ccgcagccgc gccaggggcc accgccagcg gcccccaccc caaccctccc
     3241 ccagcggggg agggagccac cgccgcgccg cgcgaggccg actggccccg ggccgatgtg
     3301 gtggtgggca atccgccgtt tctgggcgac aaatggatgc gcagcgaact cggcgatgcc
     3361 tacaccgacg ccttgcgtgc cacctacgcc gggcaggtgc ccggcggcgc ggatttcgtc
     3421 acctattggt tccacaaggc cgggcaggcc atgcgtaccc agacgctgca gcgcgccggg
     3481 ctggtggcca ccaactccat ccggcagcgc aagaaccggc aggtgctggc ccaggccagc
     3541 gcccaggccc ccatctttgc cgcctggtcc gacctgccgt gggtcaacga aggcgcggcg
     3601 gtgcgcgtct cgctggtgtg ctggggcgca ccgtcagata cccccgcgct gctggacggc
     3661 gcgcccgtgc aaagcattca ccccgacctg acggggcagg ccatcggcac ggtgtcgttt
     3721 gatctcaccc aggcgcagcc cttgcccgag aacgcgcagg cctcgtactt cgggctctgc
     3781 ctggccggcc cgttcaaggt gcccgccgcc acggcggccg agtggctgcg caaacccaat
     3841 gtgcatggcc gccccaacag cgacgtactc aagcccatct tcaacggccg cgacgtcacc
     3901 gaccgctgga gcggccaatg ggtggtggac tttggcgccc gcatgagcga acaggacgcg
     3961 gcgctgtacg aggccccgtt tcagtacgtg cagcagcatg tgctaccggt tcgggcgagc
     4021 aacaagcgtg ccgcacgcgc aaagtactgg tggcggcatg gcgaagcgcg ccccggactg
     4081 cgcgacaaat gggagggctt gagccactac atcgccaccg tcgagacggc caagcaccgc
     4141 gtgtttgtgc gtatgccgat ggcgcatgcg ccagaacaca gtctgatcgt gattccgcgc
     4201 tctgacgatg tgacgtttgg cgtcttgtcg tcgaggatgc acgttctttg ggccgtggct
     4261 cgtggaggcc gaatggggtt gggtaacgac ccgcgttaca actccctcgc tgcttttgca
     4321 cccttcccct tcccccccgg cctcaccccc caagacaccg cccaccagcg caccgaggcg
     4381 ttggccagcg gcgcgctgat ccccgcgg
//
LOCUS       DAOOOG010000198         4405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_23916_length_4405_cov_31.148736, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000198 DAOOOG010000000
VERSION     DAOOOG010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23916_length_4405_cov_31.148736"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1019)
                     /locus_tag="PK242_11795"
     CDS             complement(57..1019)
                     /locus_tag="PK242_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ94745.1"
                     /translation="MQRRSWLACLVLGWAPVAAQAPFPTRPIRLVVPAPAGDGSDALA
                     RSIAQELAAGLGQQVMIDNRPGAGGSIASDTVAKAAPDGHTLILGNGSSHGVTPSLYP
                     RLPYDALKDFAPITLVATAPNVLVVSASLPVHTVQDFLAYARARPGQLHIASGGNGSL
                     SHLSAELFKRMADVDLVHVPYKGAAPGLTDVAAGQVAAMVINIPSAQALLKAGRLQAL
                     ATTGRQRAAALPEVPTLDEAGLKGYETTSWFALLAPARTPTAVIERLQQEIARALATP
                     AVRERLAAQGLTPVGSTPAQFDAFMRAEIAKYARVIKDAGVKID"
     gene            1181..2380
                     /locus_tag="PK242_11800"
     CDS             1181..2380
                     /locus_tag="PK242_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOZ94746.1"
                     /translation="MSRGFRARPWLGVLLALGASAALLGCGGSGDAGDARLSAEAQLG
                     EKIFSDVSLSASGRQSCATCHDAARGHAANNALPAQLGGPDGTLQGVRNTPGIRYLAS
                     NTPFFFDEEGTPTGGFFWDGRAATLAEQAAGPFLNPLEMANPDKASVITKLAAAPYAA
                     EFRRVYGDAIFSDVEGAYQRLTEALQRYQLEDVEFNAFTSKYDAYLRGTAQLSAQEAR
                     GLALFNEPTKGNCAACHPSAPGVQGALPLFTDFTYDNLGLPRNPALRANDDPAYHDLG
                     LCQRADLAGRDDLCGAFKVPSLRNVALRQALFHNGVFTSLRDAVRFYVLRDTQPELWY
                     PRRADGSVDKFNDLPAHYHASVNTSEAPYDRQPGDAPALNDAEIDDVVAFLRTLTDGY
                     RPPVEQP"
     gene            complement(2397..3569)
                     /locus_tag="PK242_11805"
     CDS             complement(2397..3569)
                     /locus_tag="PK242_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOZ94747.1"
                     /translation="MFRIKTYNAISVKGLERFGRERYEVGHDIAHPDAVLLRSHKLHG
                     EALPASLLAVARAGAGVNNIPVADYGQQGIVVFNTPGANANAVKELVLAGMLLATRGI
                     LPGIAYAQSLAGMDDAAAMHALLEKEKSRFAGHELKGKTLGLVGLGAIGALVADMALA
                     LGMKVLGYDPVLSVDAAWRLSNAVQRMENLPALLAHADYVSLHVPAMDATRHLINAEA
                     LRHLRPSAVLLNFARETIVDPAAVLAALQAGRLGRYVCDFPEPGFAAEPRVIALPHIG
                     ASTEESEENCAVMAADQLIDFLEHGHIVNSVNYPALRMHRAPGSCRIAIANDNVAGVL
                     GHVLSALADAGVNVLDMSNRSREALAYNLIDVASAPEPAVIDAIRRVPHVIRVRTL"
     gene            complement(3708..>4405)
                     /locus_tag="PK242_11810"
     CDS             complement(3708..>4405)
                     /locus_tag="PK242_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HOZ94748.1"
                     /translation="NGTTSGVRVPDGDWIADDRAGLTICDATSAVFAMALPWHKLDVV
                     TYSWQKVLGGEGAHGMLILSPRAVERLESYSPPWPLPKIFRMTSGGKLSEGIFQGDTI
                     NTPSMLCVADYLDALAWCERVGGVPGTIARSTANLKAIEDFVAAHPWISFLARRPETR
                     SNTSVCLSLDLPAEQVKALVKLLEKEGVAYDIGSYKDAPPGIRIWCGATVETADVQAL
                     LPWLAWAYEQVKA"
BASE COUNT          648 a         1535 c         1541 g          681 t
ORIGIN      
        1 gctgcgccgc tcttcgagaa cgctttcggc gctccaagcc cgacagcctc ctaggctcag
       61 tcgatcttca ccccggcgtc cttgatcacc ctggcgtact tggcgatttc agcgcgcatg
      121 aaggcgtcga attgcgccgg cgtgctgccc accggcgtca ggccctgggc tgccagccgc
      181 tcgcgcaccg cgggcgtggc cagtgcgcgg gcgatttcct gctgcaaccg ctcgatcacc
      241 gcggtcggag tgcgtgccgg cgccagcagt gcaaaccagg acgtggtctc gtaacccttc
      301 aggccagcct catcgagcgt ggggacctcg ggcagcgccg ccgcgcgctg ccggccggtg
      361 gtggccagcg cctgcagccg ccccgctttg agcagggcct gggccgaagg gatgttgatg
      421 accatcgccg ccacctggcc ggcggccacg tcggtcaggc cgggcgcggc gcctttgtag
      481 ggcacgtgca ccagatcgac atccgccatg cgtttgaaca gttcggccga caggtgcgac
      541 aggctgccgt tgccgcccga ggcaatgtgc aactgcccgg gccgcgccct ggcgtaggcg
      601 aggaaatcct gcacggtgtg taccggcagg ctggcgctga ccaccagcac gttcggcgct
      661 gtggccacca gggtgatggg tgcgaagtcc ttcagcgcgt cgtagggcag cctggggtac
      721 agactgggcg tgacgccgtg cgagctgccg ttgcccagga tcagggtgtg gccgtccggc
      781 gccgccttgg ccacggtgtc ggacgcgatg ctgccgccgg cgcccggacg gttgtcgatc
      841 atcacctgct ggcccagacc agcagccagc tcctgtgcga tcgaacgcgc cagcgcgtcg
      901 gagccatcgc cggccggcgc cggtaccacc agccggatcg gcctggtcgg aaacggtgcc
      961 tgcgccgcaa cgggcgccca gcccagcacg aggcaggcga gccatgatct gcgctgcatg
     1021 atcggagttg gtgacaggtg gttggacgta ggcgcagcat acccgtcgcc ggccgcaccc
     1081 gacagaaaag gtcaagaaag gataaaaaca agaacgattc gtgtttacaa tcccgcccgc
     1141 agcgccgtgg tcgctggcgc gatggatgga ggagtgacgc atgtcccgtg gtttccgggc
     1201 ccgcccatgg ttgggtgttc tgctggcgct gggtgccagc gccgcgctgc tgggctgcgg
     1261 cggcagcggt gacgccggtg acgctcggct gtcggccgag gcgcagctgg gagaaaagat
     1321 tttctcggac gtctcgctgt cggcctcggg ccgccagtcc tgcgccacct gccatgacgc
     1381 ggcgcgcggc catgccgcca acaatgcgct gccggcgcag ctgggcgggc cggacggcac
     1441 gctgcagggc gtgcgcaaca cgcctggcat ccgctacctg gccagcaaca ccccgttctt
     1501 cttcgacgag gagggcacgc ctaccggcgg cttcttctgg gacgggcgcg ccgccaccct
     1561 ggccgagcag gccgccgggc ccttcctcaa cccgctggag atggccaatc ccgacaaggc
     1621 cagcgtcatc accaagctgg ccgccgcgcc gtacgcggcc gagttccgcc gcgtctatgg
     1681 cgacgcgatc ttcagcgatg tcgagggcgc ctaccagcgc ctgaccgagg ccctgcagcg
     1741 ctaccagctg gaagacgtgg agttcaacgc cttcaccagc aagtacgacg cctacctgcg
     1801 cggcacggcc cagctgagcg cgcaggaggc gcgcggcctg gcgctgttca acgagccgac
     1861 caagggcaac tgcgccgcct gccacccctc ggcgcccggc gtccagggtg cgctgccgct
     1921 gttcaccgat ttcacctacg acaacctggg tctgccgcgc aacccggcgc tgcgggccaa
     1981 cgacgatccg gcctaccacg acctggggct gtgtcagcgc gccgatctgg ccgggcgcga
     2041 cgacctgtgc ggcgccttca aggtgccctc gctgcgcaac gtggcgctgc gccaggcgct
     2101 gttccacaac ggcgtgttca cctcgctcag ggacgcggtg cgcttctacg tgctgcgcga
     2161 cacccagccc gagctgtggt atccgcgccg cgccgacggc agcgtcgaca agttcaacga
     2221 cctgccggcc cactaccacg ccagcgtcaa caccagcgaa gcgccctacg accgccagcc
     2281 gggcgacgcg ccggccctca acgacgccga gatcgacgac gtggtggcct tcctgcggac
     2341 gctgaccgac ggctaccgcc cgcccgtgga acagccctga agcggcgcgg cccgggctac
     2401 agcgtgcgca cgcggatcac gtgcggcacg cggcggatgg cgtcgatcac cgccggctcg
     2461 ggggccgatg cgacgtcgat caggttgtag gccagcgcct cgcggctgcg gttgctcatg
     2521 tccagcacgt tgacgccggc gtcggccagc gccgacagca catggcccag cacgccggcc
     2581 acgttgtcgt tggcgatggc gatgcgacag gagccgggcg cgcggtgcat gcgcagcgcc
     2641 gggtagttga cgctgttgac gatgtggccg tgctccagga agtcgatcag ctggtcggcc
     2701 gccatgacgg cgcagttttc ttccgactcc tcggtgctgg cgccgatgtg gggcagggcg
     2761 atcaccctgg gctcggcggc aaagcccggc tcgggaaaat cgcacacgta gcgccccagc
     2821 cggccggcct gcagcgccgc cagcacggcg gccggatcga cgatggtttc tcgcgcgaag
     2881 ttcagcagca ccgcgctggg ccgcaggtgg cgcagcgcct cggcgttgat caggtggcgc
     2941 gtggcgtcca tggccggcac gtgcaggctc acgtagtcgg cgtgcgccag cagcgccggc
     3001 aggttttcca tgcgctgcac cgcgttggac aggcgccagg cggcgtcgac cgacagcacc
     3061 gggtcgtagc ccagcacctt catgcccagc gccagcgcca tgtcggccac cagcgcgccg
     3121 atggcgccca gaccgaccag gcccagcgtc ttgcccttga gctcgtggcc ggcaaagcgg
     3181 gacttttcct tttccagcag tgcgtgcatg gcggcggcgt cgtccatgcc ggccagcgat
     3241 tgcgcgtagg cgatgcccgg caggatgccg cgcgtggcca gcagcatgcc ggccagcacc
     3301 agttccttga cggcgttggc gttggcgccg ggcgtgttga ataccacgat gccctgctgt
     3361 ccgtagtcgg ccaccgggat gttgttgacg ccggcgccgg cgcgcgccac cgccagcagc
     3421 gacgccggca gcgcctcgcc gtgcagcttg tggctgcgca gcagcaccgc gtcggggtgg
     3481 gcgatgtcgt ggccgacttc gtagcgctcg cgcccgaagc gctccagccc ctttaccgag
     3541 atggcgttgt aggtcttgat ccggaacata agatgctcac atattaatag ctgtttgcgc
     3601 agactgggtg cgccgcaggg gcatattcga tcagaacaac cttgaccctc ggaaggcggg
     3661 gcgcagccgg cggatgcgcc atgcgtgcgc ctcgacgcgg gttcgtgtca ggctttgacc
     3721 tgttcgtagg cccaggccag ccagggcagc agcgcctgca cgtcggcggt ttccaccgtg
     3781 gcgccgcacc agatgcggat gccgggcggc gcgtccttgt acgagccgat gtcgtaggcc
     3841 acgccttcct tttccagcag cttgaccagg gccttgacct gctcggccgg caggtcgagc
     3901 gacaggcaca cgctggtgtt ggagcgcgtc tcgggcctgc gggccaggaa cgagatccag
     3961 gggtgggcgg cgacgaagtc ctcgatcgcc ttcaggttgg ccgtgctgcg ggcgatggtg
     4021 ccgggcacgc cgcccacgcg ctcgcaccag gccagggcgt ccaggtagtc ggccacgcac
     4081 agcatgctgg gggtgttgat ggtgtcgccc tggaagatgc cctcgctcag cttgccgccg
     4141 ctggtcatgc ggaagatctt gggcagcggc cagggcgggc tgtagctctc cagccgctcg
     4201 accgcgcgcg ggctgaggat cagcatgccg tgcgcgccct cgccgcccag caccttctgc
     4261 cacgagtagg tgaccacgtc cagcttgtgc cagggcagcg ccatcgcgaa cacggcgctg
     4321 gtggcgtcgc agatggtcag accggcgcgg tcgtcggcga tccagtcgcc atccggcacg
     4381 cgcacgccgc tggtggtgcc gttcc
//
LOCUS       DAOOOG010000199         4228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_25241_length_4228_cov_33.565780, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000199 DAOOOG010000000
VERSION     DAOOOG010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25241_length_4228_cov_33.565780"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(490..2052)
                     /locus_tag="PK242_11815"
     CDS             complement(490..2052)
                     /locus_tag="PK242_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOZ94749.1"
                     /translation="MGLSLIQSRTLMGLQAPAVTVEVHLASGLPSFTLVGLAEVEVKE
                     ARERVRSALLNSGLQFPHNQRITVNLAPADLPKDSGRFDLPIALGILAASGQIDGGRL
                     AGWEFAGELSLSGELRPVRGALALSVARQQAGVDTRLVLPPGSAGEAALVPGQVVYRA
                     RHLLDVVARFQPPHSQPPMQDSAFGPEDEVDAGWLRVPESPRPAAPAYPDLADVKGQA
                     AARRALEIAAAGGHSLLMVGPPGTGKSMLAQRFAGLLPPMDVDEALQSAAIASLDGSF
                     RLAHWGRRPTRAPHHSASAVALVGGGSPPRPGEISLAHQGVLFLDELPEFPRAALEAL
                     REPLESGHITISRAAQRAEFPARFQLIAAMNPCPCGYLGSRLKACRCTPDQVARYQGR
                     LSGPLLDRIDLHVEVGALAPEELLNAPAGESTAAVRARCVAARERALARQGQPNQALQ
                     GQAIDTHARLDAAARTFLHSAAARLGWSARATHRALRVARSIADLAGSEAVSLAHVAE
                     AVQYRRALSGLG"
     gene            2254..3018
                     /locus_tag="PK242_11820"
     CDS             2254..3018
                     /locus_tag="PK242_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HOZ94750.1"
                     /translation="MHPTLSPLTPRRLALGLLCASAAGAWAQSSGAAAPATPEHSLSF
                     NVGLTSDYRYRGISQSRLKPAVSAGADYSHASGLYAGAWLTSIKWIKDAGGDAAAELD
                     LYAGYKGSAGPLAYDVGVLRYQYPRAHLAVSPNTTEIYGALSYGVFTAKYSHALTNLF
                     GFADSKGSGYLDLSANLDLGDGWSVVPHVGRQWVRHNSAFSYTDYAVTLNKDFGHGLV
                     ASAAVVGSNADKALYVTPSGRFTGRTALVLGLKYTF"
     gene            3079..3417
                     /gene="glnK"
                     /locus_tag="PK242_11825"
     CDS             3079..3417
                     /gene="glnK"
                     /locus_tag="PK242_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOZ94751.1"
                     /translation="MKLVTAIIKPFKLDEVREALSALGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVIDFLPKVKLETAVADDQVERVIEAIEAAARTGKIGDGKIFVAALEQVVRI
                     RTGETGADAL"
     gene            3438..>4228
                     /locus_tag="PK242_11830"
     CDS             3438..>4228
                     /locus_tag="PK242_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HOZ94752.1"
                     /translation="MKHPIAPLTLAFGLALALPGAAWAQAASDAAAAAPALAASAAAA
                     ASAAAAAAPAPKIDTGDTAWMLTSTLLVILMTIPGLALFYGGLGRAKNMLSVLVQVFV
                     IFALVSVLWALYGYSLAFSGEGNFFGGLDKLFLKGVTPETFGALTTIPEYVFLAFQGT
                     FAAITVALIVGAFAERARFSAVLLFAVLWFTFSYVPMAHIVWGGGLLAKDGALDFAGG
                     TVVHINAGIAGLVGAYLVGKRIGFGREALAPHSLTLTMVGASLLWV"
BASE COUNT          619 a         1537 c         1462 g          610 t
ORIGIN      
        1 cacttctacg acacgggccc gccgccgggt cgcgcgtcat cctaggaggc tgtcgaactt
       61 ggaaatcgcc ggctgcaaat cgaccgcagc ggcccatttt tggcttcgcc gctcttcgag
      121 agcaccggct ttttgcccaa tagctcaact attgggctgc aaatccggca aaaactggtc
      181 tcgctggggc cgattctcgc tttcggcgct ccaagcccga cagcctccta gaaacgacag
      241 ttggatgcgc ctgcccgtgc cgtgatcggt gtgccaggca agggagacga cgcagcgcac
      301 tgctgtgcgc aagaaggagc cacgccgcat ggcgcgccga gcgcggtgcg ggtgcgtgcg
      361 tagcgtgctg gcccaagagg tgaagaccat cgcagccgcc ggtcccggca tcgatgcggt
      421 ctgcccgaag ttgacaagcg gtgaactgcg gtttccgggg tcatccgcca ggtggcgagc
      481 gcatgccgat cagccgaggc cgctcagcgc gcgccggtac tgcaccgcct cggccacatg
      541 ggccaggctg accgcctcgc tgccggccag gtcggcgatg ctgcgcgcca cccggagcgc
      601 gcggtgggtg gcgcgcgcgc tccagcccag gcgggcggcg gcgctgtgca ggaaggtgcg
      661 ggcggcggcg tcgagccggg cgtgggtgtc gatggcctgg ccctgcagcg cctggttggg
      721 ctggccctgg cgcgccagcg cgcgctcgcg cgcggccacg cagcgcgcgc gcacggcggc
      781 ggtcgactcg ccggccggcg cgttcagcag ctcctcgggc gccagcgcgc cgacctcgac
      841 gtgcaggtcg atgcggtcga gcagcggccc cgacagccgg ccctggtagc gcgccacctg
      901 gtcgggcgtg cagcggcagg ccttcaggcg tgagcccagg tagccgcagg ggcaggggtt
      961 catggccgcg atgagctgaa agcgcgccgg aaactcggcc cgctgcgccg cccggctgat
     1021 ggtgatgtgg cccgactcca gcggctcgcg cagcgcctcc agcgcggcgc gcggaaattc
     1081 cggcagctcg tccagaaaca gcacgccctg gtgcgccagc gagatctcgc ccggccgcgg
     1141 cggcgaaccc ccgcccacca gcgccaccgc gctggccgaa tggtgcggcg cgcgcgtcgg
     1201 ccgccggccc cagtgcgcca gccggaagct gccgtccagg ctggcgatgg cggcgctctg
     1261 cagcgcctcg tccacgtcca taggcggcag caggccggca aagcgctgcg ccagcatcga
     1321 cttgccggtg cccggcgggc cgaccatgag cagcgaatgg ccaccggcgg cggcgatttc
     1381 cagcgcccgc cgcgccgccg cctggccctt gacgtcggcc aggtcggggt aggccggcgc
     1441 ggccgggcgc ggcgactcgg gcacgcgcag ccagccggcg tcgacctcgt cctcggggcc
     1501 gaacgccgag tcttgcatcg gcggctggct gtgcggcggc tgaaagcgcg ccaccacgtc
     1561 cagcaggtgg cgcgcccggt acaccacctg accgggcacc agcgccgcct cgccggcgct
     1621 gccgggcggc agcaccagcc gggtgtcgac gccggcctgc tggcgcgcca cgctcagcgc
     1681 cagcgcgccg cgcaccgggc gcagctcgcc cgacagcgac agctcgccgg caaattccca
     1741 gccggccagc cggccgccgt cgatctggcc gctggccgcc aggatgccga gcgcgatcgg
     1801 caggtcgaag cgccccgagt ccttgggcag gtcggccggc gccaggttga cggtgatgcg
     1861 ctggttgtgc ggaaactgca ggccactgtt gagcagcgcg ctgcgcacgc gctcgcgcgc
     1921 ctccttgacc tcgacctcgg ccagccccac cagggtgaag ctgggcaggc cactggccag
     1981 gtgcacctcg accgtgacgg cgggcgcttg cagccccatc agcgtgcggc tctgtatcag
     2041 actcaggccc atgcggcatc tcccccaaag ggtgcgtgca ccgacttgcg gcacccgcga
     2101 cagaaaaaaa agcaccgctt tggtgcagac cgcccggcga cgggcgccgc cacccgaggc
     2161 gctggcctga aatgtagcgg gcgcgagggc tggcacgcgg cgtgcttggt gtgccatgtc
     2221 ccattttttt tgatgtcacc ggagagcaag gatatgcacc ccaccctgtc acccctgacc
     2281 ccccgccgcc tcgcccttgg cctgctgtgc gccagcgccg ccggcgcctg ggcccagagc
     2341 agcggcgccg cggcgccggc cacgcctgag cacagcctca gcttcaacgt cggcctgacc
     2401 agcgactacc gctaccgcgg catctcgcag agccggctca agccggccgt cagcgccggc
     2461 gccgactaca gccacgccag cggcctgtac gccggcgcct ggctgacgtc gatcaagtgg
     2521 atcaaggacg ccggcggcga cgccgcggcc gagctcgacc tgtacgccgg ctacaagggc
     2581 agcgccggcc cgctggccta cgacgtgggc gtgctgcgct accagtaccc gcgcgcccac
     2641 ctggccgtca gccccaacac caccgagatc tacggcgcgc tcagctacgg cgtgttcacg
     2701 gccaagtatt cgcatgcgct gaccaacctg ttcggctttg ccgacagcaa gggcagcggc
     2761 tatctggacc tgtcggccaa cctcgacctg ggcgatggct ggtcggtggt gccgcacgtc
     2821 gggcgccagt gggtgcgcca caacagcgcc ttctcctaca ccgactacgc cgtcacgctg
     2881 aacaaggact tcggccacgg cctggtggcc agcgccgccg tggtgggcag caacgccgac
     2941 aaggcgctgt acgtgacgcc gtccgggcgc ttcaccggtc gcaccgcgct ggtgctcggc
     3001 ctgaagtaca cgttctgatt caaaaccgcc gctggtgctt gtccattcag cgccagaagc
     3061 tatcaaaaag gaagcatgat gaaactggtc actgccatca tcaaaccctt caagctcgac
     3121 gaggtgcgcg aggcgctgtc ggcactgggc gtgcagggca tcaccgtcac cgaggtcaag
     3181 ggcttcggcc gccagaaggg ccacaccgag ctgtaccgcg gcgccgagta cgtgatcgac
     3241 tttctgccca aggtgaagct ggaaaccgcg gtcgccgacg atcaggtcga gcgcgtcatc
     3301 gaggccatcg aagcggccgc acgcaccgga aagatcggcg acggcaagat ttttgtcgcc
     3361 gcgctggaac aagtcgtccg catccgcacc ggcgaaaccg gtgcggacgc actgtgaacc
     3421 cctgcaagga catggccatg aaacacccca ttgctcccct caccctggcc ttcgggctgg
     3481 cgctggccct gccgggcgcc gcctgggccc aggccgccag cgacgccgcc gccgccgcgc
     3541 cggcactggc cgcttcggcc gctgccgccg cttcggccgc ggcggcggcg gcgccggcac
     3601 ccaagatcga caccggcgac accgcctgga tgctgacctc caccctgctg gtgatcctga
     3661 tgaccatccc cggcctggcg ctgttctacg gcggcctggg ccgcgccaag aacatgctga
     3721 gcgtgctggt gcaggtgttc gtcatcttcg cgctggtcag cgtgctgtgg gcgctgtacg
     3781 gctacagcct ggccttcagc ggcgagggca acttcttcgg cgggctggac aagctgttcc
     3841 tcaagggcgt gacgcccgag accttcggcg ccctgaccac catccccgag tacgtgttcc
     3901 tggccttcca gggcaccttt gccgccatca cggtggcgct gatcgtcggc gccttcgccg
     3961 agcgggcgcg cttttctgcc gtgctgctgt ttgccgtgct gtggttcacc ttcagctacg
     4021 tgccgatggc gcacatcgtc tggggcggcg gcctgctggc caaggacggc gcgctggact
     4081 ttgccggcgg caccgtggtg cacatcaacg ccggcatcgc cgggctggtg ggcgcctacc
     4141 tggtgggcaa gcgcatcggc ttcggccgcg aggcgctggc cccgcacagc ctgacgctga
     4201 ccatggtggg cgcctcgctg ctgtgggt
//
LOCUS       DAOOOG010000200         4192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_25506_length_4192_cov_27.511965, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000200 DAOOOG010000000
VERSION     DAOOOG010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25506_length_4192_cov_27.511965"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PK242_11835"
     CDS             <1..270
                     /locus_tag="PK242_11835"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOZ94753.1"
                     /translation="LLAAAAAGQADAARAALEQGAAVNATDSAGRTALMLTARRGNVG
                     IARTLVDAGADPRQPDRQGLTAIDHARRAGHEALARWLEERARPR"
     gene            347..1132
                     /locus_tag="PK242_11840"
     CDS             347..1132
                     /locus_tag="PK242_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ94754.1"
                     /translation="MTATSHPPICFGLQGRVVIVTGAAQGIGEACARRFAQEGAQVVL
                     ADIADAPGQALAQALGASYVHCDVGDKAQVDALVAQVLQAHGRIDVLVNNAGIFKAAP
                     FLQVTEDDFDAVLRVNLKGAFLVGQAVARPMAEAGRGSIVNMSSVNSVLAIPDIASYN
                     VSKGGINQLTRAMALALADRGVRVNAVGPGTIATELAAQAVLTSEDARHRIMMRTPMK
                     RLGEPREIADVVAFLASDAASYITGEIVMVDGGRMALNYTVPV"
     gene            1138..1962
                     /locus_tag="PK242_11845"
     CDS             1138..1962
                     /locus_tag="PK242_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOZ94755.1"
                     /translation="MLIAHLTDTHLGIDSGALGGRLDPAAALRRALARVRALEPAPAV
                     LLLTGDLAEAGRAADYTLLAELLKQELPAHAGQAPRVLCIIGNHDHRGHARQTLGALM
                     PIAPDAPPPLACVRAEVGGLQFIGLDTVLPGHPHGALDAPQLDWLARQLRACAGQPVA
                     LFMHHPPLTSGIEAMDRCGLRRGRAELARLVAAHGGVQFIACGHLHRPIAGVLGGAPV
                     LVAPSTSHQLELDLRPGAPLALRLEPPMLGLYRWTAQDGVSCHFGHVQTFEGPYPI"
     gene            2193..3725
                     /locus_tag="PK242_11850"
     CDS             2193..3725
                     /locus_tag="PK242_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ94756.1"
                     /translation="MSLAHLLLRQAQRQPQAPAILDGAQPHASYLQWAARSAGLARHL
                     RDGGLQPGDRVLLFAHNHPRYLELLWGLWWAGLVAVPVNAKLHPREVEWIAADAGAQL
                     AFVTRDVAPDALAGVAHQVDLESMEADQLLAPVAEPAAVPITERAPDDIAWLFYTSGT
                     TGRPKGVMLTQRNLMTMGLGYFADVDPIARGDAIVYAAPISHGAGIYAIPHLMAGARH
                     VVPASRGVDAAELFALGRAVGPLSMFAAPTIVARLVDHAAQQGLGPELCARSFKTIVY
                     GGAPMYAADIQRALSVMGPRFVQIYGQGETPMVGTVLSRADLKDRAHPRHAERIASVG
                     VAQTPVRVRVTDAQGQDLPLGEVGEVLIQGDSVMAGYWGNPQATAAAIRNGWLFTGDM
                     GALDEGGYLTLKDRSKDLIISGGSNIYPREVEEALLTAAGVAEVAVVGQPDPEWGENV
                     VAFVVAQPGAAPTQEALDAHCLQRIARFKRPKRYVFVPELPKNNYGKVLKTELRRWLA
                     SA"
     gene            complement(3885..>4192)
                     /locus_tag="PK242_11855"
     CDS             complement(3885..>4192)
                     /locus_tag="PK242_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HOZ94757.1"
                     /translation="QAKYDSLSPKLKKVIDDNSGLAASAWAGEEGFDNIVGPYSKLAR
                     DRGNTIAVIEPAELARWEKATADVDEIWLKDAAAKGYDGKKLIEEAKALLKKYAPAK"
BASE COUNT          597 a         1543 c         1488 g          564 t
ORIGIN      
        1 ctgctggccg ccgccgccgc cggccaggcg gacgccgccc gcgccgcgct ggaacagggc
       61 gccgccgtca acgccaccga cagcgccggc cgcaccgcgc tgatgctgac ggcgcggcgc
      121 ggcaacgtcg gcatcgcgcg cacgctggtg gacgccgggg ccgacccgcg ccagcccgac
      181 cgccagggcc tgaccgcgat cgaccacgca cgccgcgccg gccacgaggc gctggcgcgc
      241 tggctggaag agcgggcgcg cccacggtga gcgcgcgcct gcggccacag gctgctaagc
      301 tgcgcctgcc catccacccc gacccgcagc gaccaacgca gccgccatga ccgccacctc
      361 ccatcccccc atctgtttcg gcctgcaagg ccgcgtggtc atcgtcaccg gcgccgcgca
      421 gggcatcggt gaagcctgcg cgcgccgctt cgcccaggaa ggcgcgcagg tggtgctggc
      481 cgacatcgcc gacgcacccg gccaggcgct ggcgcaggcg ctgggcgcca gctacgtcca
      541 ttgcgacgtc ggcgacaagg cgcaggtcga cgccctggtg gcccaggtgc tgcaggcgca
      601 cggccgcatc gacgtgctgg tcaacaacgc cggcatcttc aaggcggcgc cttttctgca
      661 ggtgacggaa gacgacttcg acgccgtgct gcgcgtcaac ctcaagggcg ccttcctggt
      721 cggccaggcg gtggcgcggc cgatggccga ggccggccgc ggcagcatcg tcaacatgag
      781 cagcgtcaac agcgtgctgg ccatccccga catcgccagc tacaacgtca gcaagggcgg
      841 catcaaccag ctcacgcgcg ccatggcgct ggcgctggcc gaccgcgggg tgcgcgtcaa
      901 cgccgtcggc cccggcacca tcgccaccga gctggcggca caggcggtgc tgaccagcga
      961 agacgcgcgc caccggatca tgatgcgcac ccccatgaag cgcctgggtg agccgcgcga
     1021 aatcgccgac gtggtggcct ttctggccag cgacgcggcc agctacatca cgggcgagat
     1081 cgtcatggtc gacggcgggc gcatggcgct gaactacacc gtcccggtgt aggcgccatg
     1141 ctgatcgcgc acctgacgga cacccacctg ggcatcgact ccggtgcgct gggcggtcgc
     1201 ctggacccgg ccgccgcgct gcggcgcgcg ctggcacggg tgcgcgcgct ggagcccgcg
     1261 ccggccgtgc tgctgctgac cggcgatctg gccgaagccg gccgcgcagc cgactacacc
     1321 ctgctcgccg agctattgaa acaggagctg ccggcgcatg ctggtcaagc gccgcgggtg
     1381 ctttgcatca tcggcaacca cgaccaccgg gggcacgcgc gccagaccct gggcgcgctc
     1441 atgccgatcg cccccgacgc cccgccgccg ctggcctgcg tgcgcgccga agtcggtggc
     1501 ctgcagttca tcggcctgga cacggtgctg cccggccacc cgcacggcgc gctggacgcg
     1561 ccccagctcg actggctggc gcgccagctg cgagcctgcg ccggccagcc ggtggcgctg
     1621 ttcatgcacc acccgccgct gaccagcggc atcgaggcca tggaccgctg cggcctgcgg
     1681 cgcggacgcg ccgagctggc ccgcctggtg gccgcgcacg gcggcgtgca gttcatcgcc
     1741 tgcggccacc tgcaccggcc gatcgccggc gtgctgggcg gcgcgccggt gctggtggcg
     1801 ccgtccacct cgcaccagct ggagctggac ctgcgccccg gcgcaccgct ggcgctgcgg
     1861 ctggagccgc ccatgctggg cctgtaccgc tggacggcgc aggacggcgt gagctgccat
     1921 ttcggccatg tgcagacgtt cgaggggccg tatccgatct gaagtggccg gcgcagccgc
     1981 tgcggcgggt cggctttggg aaccccggcg cagtgcgcgg gccgtcgcac atgctattga
     2041 aagcgcagct tgtggcgctt gacaggcaag cgcagcagcc cgattttccc atcgaccctg
     2101 gcggccggcc gtgccgcctg ccgggcagcg gcgcggtggc tcgcggcgtc gcgcacaatc
     2161 gggcgcacca cgacagaccg caaggagacc ccatgagcct ggcccatctg ctgctgcggc
     2221 aggcgcagcg ccagccgcag gcgcccgcca tcctggacgg cgcccagccg cacgccagct
     2281 acctgcaatg ggcggcacgc agcgccggcc tggcccgaca cctgcgcgac ggcggcctgc
     2341 agccgggcga tcgggtgctg ctgttcgcac acaaccaccc gcgctacctg gagctgctgt
     2401 ggggcctgtg gtgggccggc ctggtggcgg taccggtgaa cgccaagctg cacccgcgcg
     2461 aggtcgaatg gatcgccgcc gacgccggcg cccagctggc cttcgtgacg cgcgacgtgg
     2521 cgcccgacgc gctggccggc gtggcgcacc aggtcgatct ggagtcgatg gaggccgacc
     2581 agctgctggc cccggtggcc gagccggcgg cggtgccgat caccgagcgc gcgcccgacg
     2641 acatcgcctg gctgttctac accagcggca ccaccggccg ccccaagggc gtgatgctga
     2701 cgcagcgcaa cctgatgacc atgggcctgg gctacttcgc cgacgtcgac ccgatcgccc
     2761 gcggcgacgc catcgtctac gcggcgccga tctcgcacgg cgccggcatc tacgccatcc
     2821 cgcacctgat ggccggggcg cgccacgtgg tgccggcctc gcgcggggtg gacgcggccg
     2881 agctgttcgc gctcgggcgt gcggtggggc cgctgtcgat gttcgccgcg cccaccatcg
     2941 tcgcccgtct ggtcgaccat gccgcgcagc aggggctggg accggagctg tgcgcgcgct
     3001 cgttcaagac catcgtctac ggcggcgcac ccatgtacgc ggccgacatc cagcgcgcgc
     3061 tgtcggtgat gggcccgcgc ttcgtgcaga tctacggtca gggtgaaacg cccatggtcg
     3121 gcaccgtgct gtcgcgcgcc gacctcaagg accgcgccca cccgcgccac gccgagcgca
     3181 tcgccagcgt tggcgtggcg cagacgccgg tgcgggtgcg cgtgaccgac gcccaggggc
     3241 aggacctgcc gctcggcgag gtgggcgagg tgctgatcca gggcgacagc gtgatggccg
     3301 gctactgggg caacccgcag gcgacggcgg cggcgatccg caacggctgg ctgttcaccg
     3361 gcgacatggg cgcgctggac gaaggcggct acctgacgct gaaggaccgc agcaaggacc
     3421 tgatcatcag cggcggcagc aacatctacc cgcgcgaggt cgaagaagcc ctgctcaccg
     3481 ccgccggcgt ggccgaagtg gcggtggtgg gccagcccga ccccgaatgg ggcgagaacg
     3541 tggtggcctt cgtggtcgcg cagccgggcg cggcgccgac ccaggaggcg ctggacgccc
     3601 attgcctgca gcgcatcgcc cgcttcaagc gccccaagcg ctacgtcttc gtgcccgagc
     3661 tgccgaagaa caactacggc aaggttctga agaccgagct gcgccggtgg ctggccagcg
     3721 cctgagtgca tcagaagaac aaccggcctg gcggctcaga caagccaaga gttgctctat
     3781 tattgatagc tgctggcgct caccagtaaa gcgcggtcgg ccagaatgaa caaaaaaagc
     3841 cgctgcaggc agcggcttgg tgtcagggcg cgaggccgca gcccttactt ggccggcgcg
     3901 tactttttca gcagcgcctt ggcttcttcg atcagcttct tgccgtcgta gcccttggcc
     3961 gcggcgtcct tcagccagat ctcgtcgacg tcggcggtgg ccttttccca gcgcgccagc
     4021 tcggccggtt cgatgacggc gatggtgttg ccgcgatcgc gcgccagctt ggagtacggc
     4081 ccgacgatgt tgtcgaagcc ctcttcaccg gcccaggccg acgccgccag gccggagttg
     4141 tcgtcgatca ccttcttcag cttgggcgac aggctgtcgt acttggcctg gt
//
LOCUS       DAOOOG010000201         4157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_25802_length_4157_cov_23.753047, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000201 DAOOOG010000000
VERSION     DAOOOG010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25802_length_4157_cov_23.753047"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="PK242_11860"
     CDS             <1..1410
                     /locus_tag="PK242_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ94758.1"
                     /translation="EVEFHPSYHALMSAATAAGLHGAPYAAEEQAGGHAHIRRAAAFM
                     LFTELEPSILCPISMTYAVTPALRDNPAIYRDWSPQLTSRAYDPVLRLWRDKPGVTMG
                     MGMTEKQGGSDVRANTTQALPDGHDDWGARYRLTGHKWFMSAPMCDAFLVLAQSAGGL
                     SCFFLPRVLPDGTRNVLRIQRLKDKLGNKANASSEVEFDASLAWLVGEEGRGVPQILA
                     MGVMTRLDCALGTSALMRQALSQALHHTAQRAAFGKRLIEQPLMKNVLADLALESEAA
                     TALALRLACAFDRPHDEHERLLARVLTPIAKFWICKRGSHFAQEAMECLGGNGYVEEG
                     GEGVLARIYREMPVNSIWEGSGNIMALDLLRALRKGDVAAALARELAPARGQHAALDR
                     LIETLPTRVDQGADEVGARILARDVALAVQAALLAQTAPAAVFGAFCASRLKQPHDVF
                     GTLPGGTDFDALIRRAMPA"
     gene            1432..2349
                     /locus_tag="PK242_11865"
     CDS             1432..2349
                     /locus_tag="PK242_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ94759.1"
                     /translation="MIQDFKGKTAVLTGAGSGFGLECARIGAARGMNLVLVDVQQDAL
                     ERAAAEMTAAGAQVLARRVDVSSAEQMQALATEVKAHFGAPHFVFNNAGVGAGGLVWE
                     NTVADWNWVLGVDLWGVIHGVRLFVPMMLEAAAQDPAWRGHITNTASMAGLVAPPNMG
                     IYNTAKAAVVNLTETLYHDLNLVTDQIGASLLCPYFVPTGITHSQRNRPDSQAAATLT
                     RSQQIGQAMTEKAVTSGKVSAEQVAQKVFAAISANQFYVFSHESPRALGNISSRMQNM
                     VEMRNPVDPFIEAPEVGARLRAALRAPAS"
     gene            complement(2370..3545)
                     /locus_tag="PK242_11870"
     CDS             complement(2370..3545)
                     /locus_tag="PK242_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94760.1"
                     /translation="MRATILPAITMTPAAASQPPATVLAQVPAIQLPGSAGMPPADPV
                     ANPQALPSPQLDTHAGPASPALPTARSGAPRLNPAQQQELIRLRRLAEPRSGFGSTQA
                     AANAAWTLGLIELHGGLAPASPAQALVWFERAARYGRQPLAYAGLAWCAIDGCGGRPD
                     PSAAAQALAQLRPHHRGRALYLQWLLDTRTQPMNVRINGGPEGVNSLLLPFHELLESA
                     AAAGDTQARIELGLEAVANGNLKDARSHLEAAAPRSRAAAANLKLLELQVAKAAQGAA
                     SAPSEADDLYERARRAHRGIGGPPNYAEALRLYQAAASKGSAAARRMLGFITSRLQPD
                     GTVNLAWMAQLAWLDSSSTLPRLDTRSLSTMMYRDPTPLFDLLPEPWQRALTSVPSS"
     gene            complement(3680..>4157)
                     /locus_tag="PK242_11875"
     CDS             complement(3680..>4157)
                     /locus_tag="PK242_11875"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOZ94761.1"
                     /translation="MQIENTVKDRTAFPPGTNIFASVTVSRAAYLYCYLNDTHGNIVK
                     LLPNVLHPDALVSANQAIRLPDWMVPYPAFVLTSGKQGSEALMCVAANEDPMTRMPQE
                     LNASALTPIRGVGTLEDMRKHFETAVGADKMVAQTLQWRILPPPPAPPAAANGGKK"
BASE COUNT          628 a         1433 c         1500 g          596 t
ORIGIN      
        1 gaggtcgagt tccaccccag ctaccacgcc ctgatgtcgg ccgccaccgc cgccggcctg
       61 cacggcgcgc cgtacgcggc cgaggaacag gccggcggcc atgcccacat ccggcgcgcg
      121 gcggcgttca tgctgttcac cgagctcgaa ccctccatcc tgtgcccgat ctcgatgacc
      181 tacgccgtca cgcccgcgct gcgcgacaac ccggccatct accgcgactg gtcgccgcag
      241 ctcaccagcc gcgcctacga cccggtgctg cgcctgtggc gcgacaagcc cggcgtcacc
      301 atgggcatgg gcatgaccga aaagcagggc ggctccgacg tgcgcgccaa caccacccag
      361 gcgctgcccg acggccacga cgactggggc gcgcgctacc gcctgaccgg gcacaagtgg
      421 ttcatgtcgg cgccgatgtg cgacgccttc ctggtgctgg cgcagtcggc cggcggcctg
      481 tcgtgcttct tcctgccgcg cgtgctgccc gatggcacgc gcaacgtgct gcgcattcag
      541 cgcctgaagg acaagctggg caacaaggcc aacgccagct ccgaggttga attcgacgct
      601 tcgctggcct ggctggtggg cgaagaaggg cgcggcgtgc cgcagatcct ggccatgggc
      661 gtgatgacgc ggctggactg cgccctgggc accagcgccc tgatgcgcca ggcgctcagc
      721 caggcactgc accacacggc gcagcgcgcg gccttcggca agcggctgat cgagcagccc
      781 ctgatgaaga acgtgctggc cgacctggcg ctggaaagcg aagccgccac cgccctggcg
      841 ctgcggctgg cctgcgcctt cgaccgcccg cacgacgaac acgagcgcct gctggcgcgc
      901 gtgctgacgc cgatcgccaa gttctggatc tgcaaacgcg gcagccactt tgcccaggaa
      961 gccatggaat gcctgggcgg caacggctac gtggaggagg gcggtgaagg cgtgctggcg
     1021 cgcatctacc gcgagatgcc ggtcaacagc atttgggaag gctcgggcaa catcatggca
     1081 ctcgatctgc tgcgcgcgct gcgcaagggc gacgtcgccg ccgccctggc gcgcgagttg
     1141 gcgcccgcgc gcggccagca tgcggcgctg gaccggctga tcgaaaccct gcccacccgc
     1201 gtggaccaag gcgccgatga ggtcggcgcc cgcatcctgg cgcgcgacgt ggcgctggcg
     1261 gtgcaggccg cgctgctggc gcaaacggcg ccggcggcgg tgttcggcgc cttctgcgcc
     1321 tcgcgcctga aacagccgca cgacgtgttc ggcaccctgc ccggtggcac cgatttcgac
     1381 gccctgatcc ggcgcgccat gccggcctga gccggttcaa gaggaaccca catgattcaa
     1441 gacttcaagg gcaagacggc ggtgctgacc ggcgccggct ccggtttcgg gctggaatgt
     1501 gcgcgcatcg gcgccgcccg gggcatgaac ctggtgctgg tggacgtgca gcaggacgcc
     1561 ctggagcgcg ccgcagccga gatgacggcc gcaggcgccc aagtgctggc gcgccgggtc
     1621 gatgtatcca gcgccgagca gatgcaggcc ctggccaccg aggtcaaggc gcacttcggc
     1681 gcgccgcact tcgtcttcaa caacgccggc gtgggcgcgg gcggtctggt gtgggaaaac
     1741 accgtcgccg actggaactg ggtgctgggg gtggacctgt ggggcgtgat ccacggcgtg
     1801 cgcctgttcg tgcccatgat gctggaggcc gcggcgcagg acccggcctg gcgcggccac
     1861 atcaccaaca ccgccagcat ggccggtctg gtggcgccgc ccaacatggg catctacaac
     1921 accgccaagg cggcggtggt caacctgacc gaaacgctct accacgacct gaacctggtc
     1981 accgaccaga tcggcgccag cctgctgtgc ccgtatttcg tgcccaccgg catcacccac
     2041 agccagcgca accgacccga cagccaggcc gcggccaccc tgacgcgcag ccagcagatc
     2101 gggcaggcca tgaccgaaaa agccgtcacc agcggcaagg tgtcggccga gcaggtggcg
     2161 cagaaggtgt ttgccgccat cagcgccaac cagttctacg tcttcagcca cgaaagcccg
     2221 cgcgccctgg gcaacatcag cagccgcatg cagaacatgg tggagatgcg caacccggtc
     2281 gacccattca tcgaggcgcc ggaagtgggt gcccggttgc gcgcggcgct gcgtgctccg
     2341 gccagctgag gccgccgggt tgcgccggct caggacgatg gcacgctcgt gagggcgcgc
     2401 tgccacggct cgggcagcag gtcgaacagc ggcgtcgggt cgcggtacat catggtcgac
     2461 agcgagcgcg tatccagcct gggcagggtg ctggagctgt cgagccaggc cagctgggcc
     2521 atccaggcca ggttgacggt gccgtccggt tgcaggcgcg aggtgatgaa acccagcatg
     2581 cgccgcgcgg cggcactgcc cttgctggcg gcggcctgat acaggcgcag ggcctcggcg
     2641 tagttgggtg ggccgccgat gccgcgatgc gcccgccggg cgcgttcgta cagatcgtcc
     2701 gcttccgaag gggcggacgc ggccccctgg gcggctttgg ccacctgcag ctccagcagc
     2761 ttcaggttgg cggccgcggc gcgcgagcgc ggtgccgccg cttccagatg gctgcgcgcg
     2821 tccttcaggt tgccgttggc cacggcttcc agccccagct cgatgcgtgc ctgcgtgtcg
     2881 ccggcggcgg cagcgctttc cagcagttcg tgaaatggca gcagcaggct gttgacgcct
     2941 tccgggccgc cgttgatgcg cacgttcatc ggctgggtgc gggtgtccag cagccattgc
     3001 agatacagcg cccggccgcg gtggtgcggt cgaagctggg ccagggcctg cgccgcggcc
     3061 gaggggtccg ggcgaccacc gcagccgtcg atggcacacc aggccagacc ggcgtaggcc
     3121 agcggctggc gcccgtagcg cgccgcgcgc tcgaaccaga ccagcgcctg cgcgggcgaa
     3181 gccggcgcca ggcccccgtg tagctcgatc agacccaggg tccaggcggc gttggcggcg
     3241 gcctgggtgg agccgaaacc actgcgcggc tcggccaggc ggcgcagacg gatcagctcc
     3301 tgctgctgtg ccgggttgag gcgcggcgcg ccgcttcggg ccgtaggcag cgccggcgac
     3361 gcggggccgg catgcgtgtc aagctgcggt gagggcaacg cctgggggtt ggcgaccgga
     3421 tcggcggggg gcatgcccgc gctgccgggc agttggatgg ccggcacctg cgccagcacg
     3481 gtggcgggcg gttgggaagc tgcggctggc gtcatggtga tggccggcag gatcgtcgcc
     3541 cgcacggtcg ctgcctgcgc ggtcgcgccc gcgatcagca gaccgagcag acacccaccg
     3601 gcccgccagg ccctgcgaac ccgcctggca agcacggtcg gccatgcctg gaccagttca
     3661 cccatgacgc cccagcaggt catttcttgc caccgttggc ggccgcgggc ggggccggcg
     3721 gcggtgggag gatccgccac tggagcgtct gcgccaccat cttgtcggca ccgacggcgg
     3781 tttcaaaatg tttgcgcatg tcttctagcg taccgacccc gcggataggc gtcaaggcgc
     3841 tggcgttgag ctcctgtggc atgcgcgtca tcgggtcctc gttggcggcg acgcacatca
     3901 gggcttcgct gccctgcttg cccgaggtca gcacgaacgc cgggtagggc accatccagt
     3961 cgggcaggcg aatcgcctga ttggcagaca ccagcgcgtc cgggtgcagg acgttgggca
     4021 gcaacttgac gatgttgcca tgggtgtcgt tcaggtagca gtacaggtag gccgcccggg
     4081 agaccgtgac cgaggcaaag atgttggtgc cgggcgggaa cgccgtgcgg tccttgacgg
     4141 tgttttcgat ctgcatg
//
LOCUS       DAOOOG010000202         4143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_25904_length_4143_cov_23.226761, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000202 DAOOOG010000000
VERSION     DAOOOG010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25904_length_4143_cov_23.226761"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PK242_11880"
     CDS             <1..734
                     /locus_tag="PK242_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOZ94762.1"
                     /translation="GLFCPAQRKQALLHFASRKAMDIEGLGDKLVEQLVDGNVVRSLP
                     DLYRMGLVSLSALDRMAEKSAQNLLDALKRSKDTTLSRFLFALGIRHVGEATAKDLAR
                     HFGTLDALMDATPKQLAQVSDVGPVVAESVHAFFAEPHNREVVEQLRAAGVHWSEAAP
                     TPGATLPLAGQTVVLTGTLPTLTREQAQALLEAAGAKVAGSVSKKTSYVIAGAEAGSK
                     LDKARALGVPVLDEAGLQALLAGGA"
     gene            748..1680
                     /locus_tag="PK242_11885"
     CDS             748..1680
                     /locus_tag="PK242_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOZ94763.1"
                     /translation="MRNARGAAVSGLRLGIDLGGTKIEIAALDAQGGFALRQRVATPQ
                     GDYGATLRAIADLVAQAEAQLGQRGLPLGLGIPGSLSPLTGRVRNANSTALNGQPLRE
                     DLQHLLARPVAVDNDANCLALSEATDGAGAGADVVFAVILGTGVGAGIAVHGQVLHGC
                     NGVAGEWGHNPLPRAGALELALRCWCGRTACQELFLSGPGLAADHARAGGGPAAAAAI
                     VAAAQAGDAAAQASLARYVERLARALAQVINLLDPDVIVLGGGMSNVQALYAELPRLW
                     GPQVFSDVVRTRLVAARHGDSSGVRGAAWLAAAG"
     gene            1826..2296
                     /locus_tag="PK242_11890"
     CDS             1826..2296
                     /locus_tag="PK242_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94764.1"
                     /translation="MVGWHAVRVPRAWGAGALAATLALLGGCVAVPVEPYEPGAAVAY
                     PGYETTVVTPGYYSAPAPYYYGAPSVSLGVWGWSGGSRHWHDRPPRRHDWGRPPPPHH
                     DWGRPPPPRPHWGGRPDGPRPDVRPPPRPPHGQWQRPSPPRPRAPLINPEGMGG"
     gene            complement(2387..2986)
                     /locus_tag="PK242_11895"
     CDS             complement(2387..2986)
                     /locus_tag="PK242_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOZ94765.1"
                     /translation="MERLLEHKGLTTRLSDSIHQATDRATDLIRNAMGALGVPYRRGG
                     TSAETGFDCSGFVRSMYQRAVGLILPRTAAEQAASTAQITKTDLQPGDLVFFNTLRRA
                     YSHVGIYLGDGKFIHAPRAGAQVRVESMDISYWQKRFNGARRVLTRDSAAAEAPAALP
                     RVTERQWPAPSAVRLGGGSGKYALPAPAEIEQPQSGDRI"
     gene            3397..4023
                     /locus_tag="PK242_11900"
     CDS             3397..4023
                     /locus_tag="PK242_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HOZ94766.1"
                     /translation="MDEQLRIVVVAPDLAPSNPADERAMRQAERSRALRIGLLQHGFN
                     LVAVLPADALLEGHLTALQPDLVIVDAESEARDTLEHVVLATREAPRPIVLFTDDEDT
                     THVKDAMAAGVTAYVVAGLAPQRIRPILEVALARFAHEQALRTELVQARTALHERKLI
                     DRAKGLLMQRQSIAEQQAYDRLRRAAMDRGLRVAEVAQRMLDAADLLG"
BASE COUNT          596 a         1408 c         1508 g          631 t
ORIGIN      
        1 gcggcctgtt ctgcccggcg cagcgcaagc aggcgctgct gcactttgcc agccgcaagg
       61 cgatggacat cgaaggcctg ggcgacaagc tggtcgagca gctggtcgat ggcaacgtgg
      121 tgcgcagcct gcccgacctg taccgcatgg gcctggtcag cctgtcggca ctcgaccgca
      181 tggccgaaaa gtccgcccag aacctgctgg atgcgctgaa aagatcgaaa gacaccacgc
      241 tgtcgcgctt tctgtttgcg ctcggcatcc gccatgtggg cgaggccacg gccaaggacc
      301 tggcgcgcca tttcggcacg ctggacgcgc tgatggacgc cacgcccaag cagctggcgc
      361 aggtcagcga cgtcggcccg gtggtggccg agagtgtcca tgccttcttt gctgagccgc
      421 acaaccgcga ggtggtcgag cagctgcgcg ccgccggcgt gcactggagc gaggccgcgc
      481 ccacgcccgg cgccaccctg ccgctggccg gccagaccgt ggtgctgacc ggcaccctgc
      541 ccacgctcac gcgcgagcag gcccaggccc tgctcgaagc cgctggcgcc aaggtggccg
      601 gcagcgtcag caagaagacc agctacgtca tcgccggcgc tgaagccggc agcaagctcg
      661 acaaggcgcg ggccctgggc gtgccggtgc tggacgaggc cggcctgcag gcgctgctgg
      721 cgggcggggc atgaacgtgc cgcgccgatg cgcaacgcgc ggggtgctgc ggtgagcggg
      781 ctgcgcctgg ggatcgatct gggcggcacc aagatcgaga tcgccgcgct ggacgcgcaa
      841 ggcggctttg cgctgcgcca gcgcgtggcc acgccgcagg gcgactacgg cgccaccctg
      901 cgcgccatcg ccgatctggt ggcgcaagcc gaagcccagc tgggccagcg cggcctgccg
      961 ctgggcctgg gcattccagg cagcctgtca ccactgacgg gccgggtgcg caacgccaac
     1021 tccaccgcgc tcaacggcca gccgctgcgc gaggacctgc agcacctgct ggcgcggccg
     1081 gtggcggtcg acaacgacgc caactgcctg gccctgtctg aagccaccga tggcgccggg
     1141 gcgggtgccg acgtggtgtt tgcggtgatt ctgggcaccg gcgtgggtgc cggcattgcc
     1201 gtgcacggcc aggtgctgca cggctgcaac ggcgtggccg gcgaatgggg ccacaacccg
     1261 ctgccacgcg cgggcgccct cgagctggcg ctgcgctgct ggtgcgggcg caccgcctgc
     1321 caggagctgt ttctgtccgg ccccggtctg gcggccgacc atgcccgggc tggtggcggg
     1381 ccggccgcag ccgccgccat cgtggccgcc gcgcaggccg gcgatgcggc ggcgcaggcc
     1441 agcctggcgc gctatgtcga gcggctggcg cgtgcgctgg cgcaggtgat caacctgctc
     1501 gatcccgacg tcatcgtgct gggtggcggc atgagcaacg tccaggcgct ctacgccgag
     1561 ctgccgcgcc tgtgggggcc gcaggtgttc agcgacgtgg tgcgcacccg gctggtggcg
     1621 gcgcgccatg gcgattcgtc cggcgtgcgt ggagcggcct ggctggcggc cgcgggttga
     1681 cggataccgg cggtttcgat tggcaggtca gccggcgcgg cagcgggaac gttgtggggc
     1741 cgaacggtgt ctgaacatcg tcggacggct gcggggccat ggcacccttc ggtgtcgcca
     1801 gccgtccttc aagggagcaa gagctatggt tggttggcat gcggtgcggg ttccccgcgc
     1861 ctggggcgca ggtgcgctgg cggccacgct ggcgctgctg ggcggctgtg tggccgtacc
     1921 ggtcgagccg tacgagcctg gtgcggcggt ggcctatccc ggctacgaaa ccaccgtggt
     1981 cacgcccggt tactacagcg cgccggcgcc gtactactac ggcgcgccgt cggtgtcgtt
     2041 gggggtgtgg gggtggtcgg gcggatcgcg ccactggcac gatcgaccgc cacggcgtca
     2101 cgactggggc cggccgccgc caccgcatca cgactggggc agaccaccgc ctccgcgtcc
     2161 acattggggc ggtcggccgg acggtccgcg tcccgacgtc agaccgccgc cgcgcccgcc
     2221 ccatggccag tggcagcggc cgtcgccgcc gcgcccgcgc gcaccgctga tcaaccccga
     2281 agggatgggt ggctgagccc tgccgcctgc gctcctggcg cgaccaaaaa agagggccct
     2341 tgcgggcccc tacctttgga ggaaggtcaa tcggtcggtg tgcggatcag atgcggtcgc
     2401 cgctctgcgg ctgctcgatt tcggccggcg cggggagggc gtacttgccg ctgccgccac
     2461 ccaggcgcac ggcgctgggg gccggccact ggcgctcggt gacgcggggc agggcggctg
     2521 gcgcttcggc ggcggctgaa tctcgcgtca gcacccgccg cgcaccgttg aagcgcttct
     2581 gccagtagct gatgtccatg ctctcgacgc gcacctgcgc gccagcgcgt ggggcatgga
     2641 tgaacttgcc atcgcccaga tagatgccga catggctgta ggcgcggcgc agggtgttga
     2701 agaacaccag atcaccgggc tgcagatcgg tcttggtgat ctgggcggtg gaggctgcct
     2761 gttcagcggc cgtgcgcggc aggatcagtc ccacggcccg ctgatacatg ctgcgcacga
     2821 acccgctgca gtcaaatccg gtttcggccg acgtgccgcc acggcggtag ggtacgccca
     2881 gtgcgcccat ggcgttgcgg atcagatcgg tggcgcggtc ggtggcctgg tggatgctgt
     2941 cactgagcct ggtggtcagg cccttgtgtt ccagcagacg ttccatatcc tgctgcgcgg
     3001 gctcggcatg ggcctggtgc acggaggcag ccagcaggat caaggcgaca gcgaaggtct
     3061 tgggcatcag ggcgttgtga gcggcgaggt gcggcttgcg tgaggaaggc aaacgcgagg
     3121 ccgcagaaga ggttaaatcg aatactttct tatctgttcg tacccatttc tggggagtcg
     3181 tcgtagtgta ggccatggca ctgacctgcc aagccggtga catccgctcg ccatccggat
     3241 ttaccccagg attgcgtgat atgccacgcc gaaatgcgca tttcgaccag gtttgcacgc
     3301 catggcggga cgccgaaccg gcatggcccg cgccatcgcg gcgtcctgcg gctcgatcgc
     3361 gcgcaagctg gcgcggctct tgcttcgctg ttggtcatgg acgaacaact tcgcatcgtg
     3421 gtcgtggccc ccgacctggc ccccagcaac ccggccgacg agcgcgccat gcgccaggcc
     3481 gaacgctcgc gcgcgctgcg cataggcctg ctgcagcatg gcttcaacct cgtggccgtg
     3541 ctgccggccg atgcgctgct ggagggccat ctgacggcgc tgcagccgga cctggtcatc
     3601 gtcgacgccg agagcgaggc gcgcgacacg ctcgagcatg tggtgctggc cacgcgcgag
     3661 gcaccgcgcc ccatcgtgct gttcaccgac gacgaggaca ccacccatgt caaggacgcc
     3721 atggccgcgg gcgtcacggc ctatgtcgtc gcgggccttg cgccgcagcg catacgcccc
     3781 atcctcgagg tggccctggc ccgctttgcc catgagcagg cgctgcgcac cgaactggtg
     3841 caggcacgca ccgctttgca cgagcgcaag ctcatagacc gggccaaggg cctgctgatg
     3901 cagcgccagt ccatcgccga gcagcaggcc tacgaccggc tgcgccgcgc cgccatggac
     3961 cgcggcctgc gcgtggccga ggtggcgcag cgcatgctgg acgcggcaga tttgctcggc
     4021 tgagccaggg caggcaacac tggcacacca cttgctttag gtttttcagg ccaacgcagg
     4081 tctgccccgc gcactttcaa cggcgaaagc ggcgcacccc aggacaaagg cgtcctcacg
     4141 cat
//
LOCUS       DAOOOG010000203         4131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_26001_length_4131_cov_30.458292, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000203 DAOOOG010000000
VERSION     DAOOOG010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26001_length_4131_cov_30.458292"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK242_11905"
     CDS             <1..212
                     /locus_tag="PK242_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ94767.1"
                     /translation="VSAIAPGPFASEMNQSARDHADEVAARTPAGRIGTPEDMAGAAI
                     YLASRAGDYVVGSTLVVDGGVAWAR"
     gene            complement(301..1218)
                     /locus_tag="PK242_11910"
     CDS             complement(301..1218)
                     /locus_tag="PK242_11910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOZ94768.1"
                     /translation="MRQLLLDLFGQGAEPSPQSPHSKSNQAPALDGQAQTAINNIVHG
                     GDATALARPTVTGFQHPQANRRIVLGGTPVAYLFRRGKRRTIGLSIGAEGLQVSAPRW
                     TPLTEIEAALHEKTRWVLAKLAEARQRQAAQQGARIDWRDGAALPYLGAPLTLRLDPR
                     QQHGRAGAVLQSALPEGPILWLGLPASASAQQVQDVAQAWLLGQARRLFAARLDHFAP
                     LMGVRWQRLTLSSAGTRWGSASADGSIRLNWRLIHLDAALIDYVVVHELAHLHEMNHS
                     PRFWQHVGRVLPDCHERRVALKRSVVPTA"
     gene            complement(1230..2003)
                     /locus_tag="PK242_11915"
     CDS             complement(1230..2003)
                     /locus_tag="PK242_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOZ94769.1"
                     /translation="MNLIRSVLHLLWMVVTVIPWATLVVLLAPFVGKPRLYRICRAWL
                     DLTVRAGRWILGIENRVSGLQHLPPDPAAGAVLLVKHQSAWETFCMPPLMPHPLAYVF
                     KRELLSIPFFGWAIGRLDMIHIDRGQRIEAFHKVVEQGRRLLGQGVWVIMFPEGTRIP
                     RGEKGSYQSSGARLAVECGVPIIPVAVTSARCWPRKAFVKRPGVVDISIGPPIDTRGR
                     KPHELMREVESWIEAEMLRLDPEAYPGGAAVRSSVRPSA"
     gene            complement(2071..2640)
                     /gene="gmhB"
                     /locus_tag="PK242_11920"
     CDS             complement(2071..2640)
                     /gene="gmhB"
                     /locus_tag="PK242_11920"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HOZ94770.1"
                     /translation="MKLVILDRDGTICVEREGYLQSPADWEPLPGALEAIAQLNQAGW
                     QVVIAANMPGLGRGLFDMAALNAIQARMHKQLAAVGGKVDAMFFCPHTADEACSCRKP
                     LPGLFQQIGERYRVDLRQVHAVGDSERDMQAAHAAGCVPHLVLTGLHAPSHTDPLPPA
                     YPPGTRVHADLAAFASALVGAPDAAPGPA"
     gene            complement(2649..3272)
                     /locus_tag="PK242_11925"
     CDS             complement(2649..3272)
                     /locus_tag="PK242_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94771.1"
                     /translation="MSPGPAAAATTGLGWRGLTWLLAPPLAVLLLRALLQALAEADAD
                     GLQALHPATLVSDPAQALWAAARPAVFTGLGLLGAVLGLRLAGRAALRRHGWARLRPW
                     ALALWLLLWLGVGAGLVASQLNRAGRRPLPAQEVRVLLLREVPASARGPGGTEVYFEL
                     APAGAPQRLLAEGQPPTAFAPGRPARLDWQAGRWWGRWGRLTPGGGG"
     gene            complement(3269..>4131)
                     /gene="glyS"
                     /locus_tag="PK242_11930"
     CDS             complement(3269..>4131)
                     /gene="glyS"
                     /locus_tag="PK242_11930"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOZ94772.1"
                     /translation="GVMGGYYARHDGLTEDIALAIEDHYKPRFAGDELPRGQVGTVVA
                     LADKLETLVGMFGIGNLPTGDRDPFALRRHALGVIRMLIEQALPLALPELIELAAQTF
                     AGKADFNPSRVKDELAGKFIYDRLAGSLREQGFSAQEVDAVLALRPPRLADVHPHLQA
                     VRAFAALPEAPALAAANKRIGNILKKAEAESPVHPHVSELLLQEPAEQALYAAMQQVL
                     PQADAQLARGDYTASLQSLAALRAPVDAFFDHVMVNAEQADLRLNRQGLLKTLHQAMN
                     RVAELARLAA"
BASE COUNT          582 a         1477 c         1524 g          548 t
ORIGIN      
        1 tcgtcagcgc catcgcgccc ggcccgttcg cgtcggagat gaaccagagc gcgcgcgacc
       61 atgccgacga ggtggcggca cgcaccccgg ccgggcgcat aggcacgccg gaagacatgg
      121 ccggcgccgc catctatctc gcctcgcgcg ccggcgacta cgtggtcggc tcgaccctgg
      181 tggtcgatgg cggggtggcc tgggcgaggt gatgaggcgg ggtcgcgctc ggcacgatca
      241 ccgatgacaa atgccttcgc cgcgcttcga tgacccaggc ggggagcgcc cgcggacccg
      301 tcaggcggtg ggcaccacgc tgcgcttgag cgccacgcgc cgctcgtggc agtcgggcaa
      361 cacgcgcccc acgtgctgcc agaagcgcgg gctgtggttc atctcgtgca ggtgcgccag
      421 ctcgtgcacc accacgtagt cgatcagcgc cgcgtccagg tggatcaggc gccagttcag
      481 gcggatcgag ccgtcggcgc tggccgagcc ccagcgcgtg ccggccgatg acagcgtcag
      541 ccgctgccag cgcacgccca tcagcggcgc aaagtgatcg agccgcgccg caaacagccg
      601 ccgcgcctgc cccagcagcc aggcctgggc gacgtcctgc acctgctgcg cgctggcgct
      661 ggcgggcagg cccagccaca ggatggggcc ttcgggcagt gcgctctgca gcacggcgcc
      721 ggcgcggcca tgctgctgac gcgggtccag ccgcagcgtg agcggcgcgc ccaggtacgg
      781 cagcgccgcg ccatcgcgcc agtcgatgcg tgcgccctgc tgcgcggcct ggcgctggcg
      841 ggcctcggcc agcttggcca gcacccagcg cgttttctcg tgcagggcgg cttcgatctc
      901 ggtcagcggc gtccagcgcg gggcgctgac ctgcaggccc tcggcgccga tggacaaacc
      961 gatggtgcgc cgcttgccgc gccgaaacag ataggccacc ggcgtgccac ccagcacgat
     1021 gcggcgattg gcctgggggt gctgaaagcc ggtgacggtg ggcctcgcga gcgccgttgc
     1081 gtcgccgcca tgaactatat tattgatagc tgtctgcgct tgcccatcaa gcgctggagc
     1141 ctgatttgac ttcgaatgtg gcgactgcgg cgacggctcg gcgccctggc cgaacagatc
     1201 gagcagcaac tggcgcatgg gctgtcgcgc taggcgctgg ggcgcacgct ggagcgcacg
     1261 gcggcgccgc cgggataggc ctcggggtcc aggcgcagca tttcggcctc gatccaggac
     1321 tcgacctcgc gcatcagctc gtgcggcttg cggccgcggg tgtcgatggg cggaccgatg
     1381 gagatgtcga cgacgcccgg tcgcttgacg aaggccttgc gcggccagca gcgcgccgaa
     1441 gtgacggcga ccgggatgat gggcacgccg cattcgaccg ccaggcgcgc gccgctgctc
     1501 tggtagctgc ccttttcgcc gcgcgggatg cgcgtgccct cggggaacat gatcacccac
     1561 acgccctgac ccagcaggcg ccggccctgc tcgaccacct tgtgaaaggc ttcgatgcgc
     1621 tggccgcggt cgatgtggat catgtccagc cgaccgatgg cccagccgaa gaacgggatc
     1681 gacagcagtt cgcgcttgaa cacataggcc agcggatgcg gcatcagcgg cggcatgcag
     1741 aaggtttccc aggccgactg gtgtttgacc agcagcacgg cgccggcggc cgggtccggc
     1801 ggcaggtgct gcagcccgct gacgcggttc tcgatgccca ggatccagcg gccggcgcgc
     1861 accgtcagat cgagccaggc gcggcagatg cggtacaggc gcggcttgcc gacgaagggc
     1921 gccagcagca ccaccagcgt ggcccagggg atgacggtga cgaccatcca cagcaggtgc
     1981 agcacggagc ggatcaggtt catgaacgat cagatgttgg cgcccgtcgg gtatgcgcga
     2041 gcagctatga ataacggagc aaacgaggcc tcaggcgggg cctggcgcgg catcgggcgc
     2101 gccgaccagg gcgctggcga aggccgccag gtcggcgtgc acgcgcgtgc cgggcggata
     2161 ggccggcggc agcgggtcgg tgtggctggg ggcgtgcagg cccgtcagca ctaggtgcgg
     2221 cacgcagccg gccgcgtggg cggcttgcat gtcgcgttcg ctgtcgccca ccgcatgcac
     2281 ctggcgcaga tcgacccggt agcgctcgcc gatctgctgg aacagcccgg gcagcggctt
     2341 gcggcagctg cacgcctcgt cggccgtgtg cgggcagaag aacatggcgt ccaccttgcc
     2401 gcccacggcc gccagctgct tgtgcatgcg cgcctggatg gcgttgagcg ctgccatgtc
     2461 gaacagcccg cgacccaggc ccggcatgtt ggcggcgatc accacctgcc agcccgcctg
     2521 gttgagctgg gcgatggctt ccagtgcgcc cggcagcggc tcccagtcgg cgggcgactg
     2581 caggtagcct tcgcgttcga cgcagatggt gccgtcgcgg tccaggatga cgagcttcat
     2641 ggcggcgatt atccgccgcc gcccggcgtc agccggcccc agcggcccca ccagcgcccg
     2701 gcctgccagt ccagccgggc cggacggccg ggcgcaaacg cggtcggcgg ctggccctcg
     2761 gccagcaggc gctgaggcgc gccggccggg gccagctcga aatacacctc ggtgccgccc
     2821 gggccgcgtg cgctggccgg cacctcgcgc agcagcagca cgcgcacctc ctgcgccggc
     2881 agcggccgac ggccggcgcg gttcagctgg ctggccacca gcccggcgcc gacccccagc
     2941 cagagcagca gccacagcgc cagcgcccac ggccgcaggc gcgcccagcc gtggcggcgc
     3001 agcgccgcgc ggccggccag gcgcagcccc agcaccgcgc ccagcagccc cagcccggtg
     3061 aacaccgccg gccgcgccgc cgcccacagc gcctgcgccg ggtcggacac cagggtggcc
     3121 gggtgcagcg cctgcaggcc gtcggcatcg gcttcggcca gcgcctgcag cagcgcgcgc
     3181 agcagcagca ccgccagcgg cggtgccagc agccaggtca ggccgcgcca gccgaggccg
     3241 gtggtggcgg cggccgcagg ccccgggctc acgccgccag ccgcgccagc tcggccacgc
     3301 ggttcatggc ctggtgcagg gtcttgagca ggccctggcg gttcagccgc aggtcggcct
     3361 gctcggcgtt gaccatgacg tggtcgaaga aggcgtccac cggcgcacgc agcgccgcca
     3421 gggactgcag gctggcggtg tagtcgccgc gcgccagctg cgcgtcggcc tgcggcagca
     3481 cctgctgcat ggcggcgtac agcgcctgct cggccggctc ctgcagcagc agctcgctga
     3541 catgggggtg caccgggctt tcggcctcgg ccttcttcag gatgttgccg atgcgcttgt
     3601 tggccgccgc cagggcgggt gcttcgggca gggcggcaaa ggcgcgcacc gcctgcaagt
     3661 gcggatgcac gtcggccagg cgcggcgggc gcagggccag cacggcgtcg acttcctgcg
     3721 cgctgaagcc ctgctcgcgc aggctgccgg ccaggcggtc gtagatgaac ttgccggcca
     3781 gctcgtcctt cacgcgcgac gggttgaagt cggccttgcc ggcaaaggtc tgcgccgcca
     3841 gctcgatcag ctcgggcagc gccagcggca acgcctgctc gatcagcatg cggattacgc
     3901 ccagcgcatg gcggcgcagc gcgaacgggt cgcggtcgcc ggtgggcagg ttgccgatgc
     3961 cgaacatgcc gaccagggtt tccagcttgt cggccagcgc caccaccgtg ccgacctggc
     4021 cgcgcggcag ctcgtcgccg gcaaagcgcg gcttgtagtg gtcttcgatg gccagcgcga
     4081 tgtcttcggt caggccgtcg tggcgcgcgt agtagccgcc catcacgcct t
//
LOCUS       DAOOOG010000204         4124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_26067_length_4124_cov_19.567953, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000204 DAOOOG010000000
VERSION     DAOOOG010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26067_length_4124_cov_19.567953"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1459
                     /locus_tag="PK242_11935"
                     /pseudo
     CDS             1..1459
                     /locus_tag="PK242_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062316.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1560..2045
                     /locus_tag="PK242_11940"
     CDS             1560..2045
                     /locus_tag="PK242_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94773.1"
                     /translation="MRNGYALASADGLQAMGAALRALSPAQLDALRAQLRIGLHAGVE
                     VTDGDLPGTVVSQAFCSALPVAYTRVPAALWAPLAQMVLEAAYEATLLATAENAQSGG
                     SNIALLTLLGGGVFGNDAQWIHAALRRALTLARDRDLDVRIVSYAAAAPATEALVAEL
                     A"
     gene            complement(2060..2404)
                     /locus_tag="PK242_11945"
     CDS             complement(2060..2404)
                     /locus_tag="PK242_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94774.1"
                     /translation="MWICLNKAFLSIVDKDCAPDELLVRARRDGDIERVFPDAKVEVT
                     LGNDYRCRARIKREQVTQALAAEVMGIDYANFKGSTRERALHDAYMDAWGAFGQLQPG
                     GPYSTGLRRRKG"
     gene            complement(2409..3041)
                     /locus_tag="PK242_11950"
     CDS             complement(2409..3041)
                     /locus_tag="PK242_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOZ94775.1"
                     /translation="MQHPRPNDQGQPVQINKPSTPSPLQAWNDPAAIATAIPDSTMPA
                     AIHAVPVTAWRDAPQGSAGWEALARSLVFPEPPMPQAAGKKPAAGVVTIEPDGRVWVV
                     APTNGFGGYAHTFPKGKLDALSTHATAIKETWEESGLRVELTGFLCDSVRSTSVTRYY
                     LARRVGGDPAAMGWESQAVLLVPADKLDALVKHPNDAPLLARIKTHLNGG"
     gene            complement(3186..3635)
                     /locus_tag="PK242_11955"
     CDS             complement(3186..3635)
                     /locus_tag="PK242_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94776.1"
                     /translation="MTFKCHACGKPIHTTLHSLDRRVQSQAIAEVDGRVQTILHIHQA
                     DTVLLHDSLECRASHEPGVIEHLRLKTPYPPAGIVVPCSRCGAPVVRTAPHVGYAWVT
                     LELHGAQALTGHCTDDQDLAVLCPHCEPPGAAAAEEIDERTRERPVS"
     gene            complement(3751..3827)
                     /locus_tag="PK242_11960"
     tRNA            complement(3751..3827)
                     /locus_tag="PK242_11960"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3791..3793),aa:Arg,seq:tct)
     gene            complement(3881..3957)
                     /locus_tag="PK242_11965"
     tRNA            complement(3881..3957)
                     /locus_tag="PK242_11965"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3921..3923),aa:Pro,seq:tgg)
BASE COUNT          687 a         1358 c         1396 g          683 t
ORIGIN      
        1 aggaactggc cttcaagcag gccatcatcg acaagctcac ccacgagaac gcgctcttaa
       61 ggcgcatgaa gtttgcagcc cagtctgagc gcttcagtcc cgagcaaaag agcctgctcg
      121 aagacgaaat cgaagcggac ctggcggccg tggccagcca gatcgatgcg ctggcgcagg
      181 tgcaaacacc aaccaatccc gagaagaaac aggccaggcg cctgccgctg ccggcgcacc
      241 tgcctcggcg cgagatccgc cacgaaccca cgtccaccac ctgcgcctgc ggctgccaga
      301 tcaggcgcgt gggcgaagac gtggccgaga agctcgatga cgtgccaggc gtgttcagtg
      361 tcgagcgcca gatccgtggc aagtgggcct gcgcccagtg tgagaccatt caacaggtgc
      421 cggttgaggc gcacgtcatc gacaagggta tccccaccac cggcctgctg gcccaggtgc
      481 tggtgggcaa gtaccaggac cacctgccgc tgtaccggca ggaggcgatc ttcgctcgtg
      541 ccggcctggc catcccgcgc tcgacgctgg cgcaatgggt gggcacctgc ggggtgcggc
      601 tgcagccgct ggtcgaggcg ctcaagaccg agatcctcgg ccaccgagtg ctgcacgccg
      661 acgagacacc ggtggccatg cgcaagccgg gcaacggcaa gacgcaccga gcctacctct
      721 gggcttatgc cccaggggcg tttgaagaca tggaggccgt ggtctacgac ttctgcgagt
      781 ctcgctcggg cgagcatgcc cggcgcttgc tgggccatgg caccaacaag gcttggaagg
      841 gtagcctgat ctgcgacgac ttcggcggct acaaagccct gatcgccagc ggcgtgaccg
      901 aggtgggctg tctggcccat gcccggcgca agttcttcga tctgcacgcg gcgggccaga
      961 gccagatcgc cgagtttgcc ctgcaacagt tcacccgggt ctatggcatc gagcgtgaag
     1021 tcaaggaact gggcaacgaa cagcgacaag ctgttcgcca gcagcggagc tggccaatcc
     1081 tcgatgccct gcacaagtgg atgacactgc agcggtgcca ggtgcccgag gatcggccac
     1141 cgccaaagcg ctggactaca gcctgcggcg ctgggatgcg ctgacccggt tcgtggatga
     1201 cgggcaattg ccggtggaca acaactggat cgagaaccag atccggccca ttgccatcgg
     1261 acgcaacaac tggctgtttg ccggcagcct gcgtgcgggc cagcgggccg ctgccatcat
     1321 gagcctgatt cagtcggcgc gcgtgaacgg gcacgatccg tatgcctacc tcaaggatgt
     1381 gctcacgcga ttgcccacgc acagggccag ccgcattgac gagctcttgc cgcaccgctg
     1441 gcagccctca gacatctgat catcagacag gaccgtcagc ggcggtcaat atgggttcgc
     1501 cggtcggata cgccgcattg gctgaactca ccgatcgatc ggtccgagac ttatggagga
     1561 tgcgcaacgg ctacgcgctg gccagcgccg acggcctgca ggccatgggc gccgcgctgc
     1621 gggcgctgtc gccggcgcag ctcgacgccc tgcgcgccca gctgcgtatt ggcctgcatg
     1681 ccggcgtcga ggtcaccgac ggcgatctgc ccggcaccgt ggtctcgcag gcgttctgct
     1741 ccgcgctgcc ggtggcctac acccgcgtgc cggcggcgct gtgggcgccg ttggcacaga
     1801 tggtgctgga ggccgcctac gaagccacct tgctggccac cgctgaaaac gcgcaatccg
     1861 gtggctccaa catcgccttg ctgaccctgc tgggcggcgg cgtgttcggc aacgatgccc
     1921 aatggatcca cgcggcgctg cgccgggcgc tgaccctggc gcgcgaccgg gacctggacg
     1981 tgcgcatcgt cagttatgca gcggccgcac ccgccactga ggccctggtg gccgagctcg
     2041 cctgagcggc cttgggcgat cagcccttgc gcctgcgcaa acccgtgctg tacggcccgc
     2101 ccggctgcag ctgaccgaag gcaccccaag catccatata cgcatcgtgc agcgcgcgct
     2161 cgcgcgtgct gcccttgaaa ttggcgtagt cgatgcccat gacctcagcc gccagggcct
     2221 gggtcacctg ctcgcgcttg atgcgggcgc ggcagcggta gtcgttgccc agcgtcacct
     2281 cgaccttggc gtcgggaaac acgcgctcga tgtcgccatc gcgccgcgcg cgcaccagca
     2341 gctcgtcggg cgcgcaatcc ttgtccacga tggacaggaa ggccttgttc agacagatcc
     2401 acatggggtc atcctccatt caggtgagtt ttgatcctgg ccagcagggg cgcgtcgttc
     2461 gggtgcttga ccagggcatc gagcttgtcc gccggcacca gcagcaccgc ctgtgactcc
     2521 cagcccatcg ccgccggatc gccgccgact cggcgcgcca ggtagtaacg ggtcacgctg
     2581 gtgctgcgca ccgagtcgca cagaaaaccg gtcagctcga cgcgcaggcc cgactcttcc
     2641 caggtctcct tgatcgccgt agcgtgcgtg ctcagcgcat cgagcttgcc cttggggaag
     2701 gtgtgcgcgt agccgccaaa gccattggtc ggcgccacca cccagacccg gccgtcgggt
     2761 tcgatcgtga ccacgcccgc ggccggcttc ttgcccgcgg cctggggcat gggcggctcg
     2821 ggaaatacca ggctgcgggc cagggcttcc cagcctgcgc tgccctgcgg tgcgtcgcgc
     2881 catgcggtga cgggcacggc atggatggcc gcgggcatgg tgctgtccgg tatcgcggtg
     2941 gcgatggcag cgggatcgtt ccaggcctga agagggctgg gcgtgctggg tttgttgatc
     3001 tggacgggct ggccctggtc gttcgggcgg gggtgctgca tggtttcggt gaagggtcaa
     3061 tgcgaggggg cgacagtatc gcctgccggc ttggtcacgc tgccgctcga tgtattcgaa
     3121 cccatgtccc aacccgcccg gtcccaagga ttgcggggcg cgtcgtggcc catgctgagc
     3181 cggcgtcagc tcaccggccg ctcgcgcgtt cgctcgtcga tctcctcggc cgctgcggcg
     3241 ccgggtggct cgcagtgagg gcacagcaca gccaggtcct ggtcgtcggt gcagtggccc
     3301 gtcagagcct gcgcgccgtg caactcgagc gtgacccagg cgtagccaac atggggcgcg
     3361 gtgcgcacca ccggcgcccc gcaacggctg caaggcacca cgatgcccgc aggcgggtag
     3421 ggggtcttga gccgcaggtg ctcgatcacg ccgggctcgt gcgaggcgcg gcattccagc
     3481 gagtcgtgca gcaaaacggt atcggcctgg tggatgtgca ggatggtctg cacgcggccg
     3541 tccacctcgg caatcgcctg cgactgcacg cgccgatcca ggctgtgcag cgttgtgtgg
     3601 atcggcttgc cgcacgcgtg gcacttgaag gtcatgagat tgccttgctg gtgggttgga
     3661 gcgtcacgat cttgccagca ctctatcggc gcaggagccc ggaaacccgc ggccggcggg
     3721 ctgagccgtc ctgctggtga gcgtgatggc tggcctgccc ggcaggactc gaacctgcga
     3781 cctgctgctt agaaggcagc tgctctatcc agttgagcta cgggcagccg aaacgaacaa
     3841 ggcccggcaa tgggtgcgcg ggccttgatt gtgtatgaag tggtcggagc ggcgggattc
     3901 gaactcgcga ccctctgctc ccaaagcaga tgcgctacca ggctgcgcta cgctccgaca
     3961 aaaccgacat tctactgtgt caatgtccat ggacggcagg acttgacgac ggcgccgctg
     4021 gatggcgccg cagcagcact ccgaagtact attgattcaa tagctggtag cgcttgtctg
     4081 gcaagcgcca cggccatgaa aggcgtttca gctgccgcgc gtga
//
LOCUS       DAOOOG010000205         4073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_26513_length_4073_cov_30.367347, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000205 DAOOOG010000000
VERSION     DAOOOG010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26513_length_4073_cov_30.367347"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PK242_11970"
     CDS             complement(<1..763)
                     /locus_tag="PK242_11970"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOZ94777.1"
                     /translation="MTRKFIDLSIYLENDVVSDPPAFAPKIQYFNHQNSFEQMAPFFP
                     GLKQSDLPDGEVWAVEMVQLSTHNGTHLDAPYHFHSTMDKALGGKKPAWTIDEVPLEW
                     CFQPGVKLDFRHFGDGYVVTAADVEAELKRIGHELKPLEIVVVNTRAGSRYGHNDYVA
                     AGCGMGYEATMYLLERGVRLTGTDAWSWDAPFVYTAQKYAESKDAGLIWEGHKAGRDI
                     GYCHLEKLHNLEALPADGFYISCFPHKIRGASAGWT"
     gene            complement(778..1815)
                     /locus_tag="PK242_11975"
     CDS             complement(778..1815)
                     /locus_tag="PK242_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOZ94778.1"
                     /translation="MPANPLRGFAVDRAQIRRIGHDLQRPECILAEPDGTLWSADARG
                     GVMRIAPDGTQRFIGQRADARFGSAAAASSAELEHKFTTGTLPNGLAFAANGDILISN
                     FGTDRLEVMTRGGDTRVLYDRIDGRPIGKVNFVLRDSKDRIWITVSTRVNPWTQAAAS
                     RVQDGCIAVVERGVLRVVAEGFFFTNEIRLDAREEWLYIVETTGPHISRMRVVEGAQG
                     VALTDREVFGPAHLGGYPDGIAFDAFGNLWCTLVMVDQLIALTPEGERLLLLDDGNPQ
                     ASQALLAHMAAGTVTAEAMMAARGTLAPWMASVTFGGPDLRTVYIGSLMGTSIPYFRS
                     PVAGLPMVHWK"
     gene            complement(1818..3149)
                     /locus_tag="PK242_11980"
     CDS             complement(1818..3149)
                     /locus_tag="PK242_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOZ94779.1"
                     /translation="MSPIAVACLSLLAMLGLIWAGMHVAVVLALVSFVGVWLIRGDMD
                     VAANLLAQASQDAVASHIFGVVPLFVLTGFLVARADVGKDAFEVANQVLRRVRGGLGV
                     ATVASNAVFAAITGISIASAAVFTRVAVPEMLRFGYQPRFAVGVVAGSSVLGMLIPPS
                     LLMILYGFLANQSVGDLFTAGIVPGLVLALAYSLGIVLMATLWPRSVYAGGQAQPAQE
                     AALLPVGTLALKLLPSVLLIASVLGGLYAGFFTATEAGATGAAVALAVALLRRKLDWR
                     TLWGVLTETGHVTVSVSFLIICATIYTRMLAMSGLPQFLVEWMTQLGLGPTGFLLIYI
                     GFIILLGMIIDSSSILLIVLPLMVPIAEQFGMNLIWLGVVTVVAVEIGLLTPPFGLSV
                     FVIKSTLSDSRISLGDIFRGTLPFTLMMLAVLLLLVFVPQLSLLLLKTPGG"
     gene            complement(3208..3756)
                     /locus_tag="PK242_11985"
     CDS             complement(3208..3756)
                     /locus_tag="PK242_11985"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOZ94780.1"
                     /translation="MKSEVTDAYGQPLPGRFERLTQALNALGTLWIVALMLLINADVL
                     GRELLNAPLRGTTELVSLSIVGIVFLQLADTLASGRLTRADVLLDRLKRTTPWLAALL
                     QALYHLVGAGLMAVILWAAWAPLVESIRIQEYVGALGDFTAPVWPVRLIMLVGMVMTL
                     LTFVLLAWLDLRRLRAGLKGRS"
BASE COUNT          702 a         1434 c         1364 g          573 t
ORIGIN      
        1 gcgtccagcc ggccgaggcg ccgcggatct tgtgcggaaa gcagctgatg taaaagccgt
       61 cggccggcag cgcctccagg ttgtgcagct tttccaggtg gcagtagccg atgtcgcgcc
      121 cggccttgtg gccttcccag atcaggccgg cgtccttgga ctcagcgtac ttctgcgcgg
      181 tgtagacgaa gggcgcgtcc cagctccagg cgtcggtgcc cgtcaggcgc acgccgcgct
      241 ccagcaggta catggtggcc tcgtagccca tgccgcagcc ggcggccacg tagtcgttgt
      301 gaccgtagcg gctgccggcg cgggtgttga cgaccacgat ctccagcggc ttgagttcgt
      361 ggccgatgcg cttcagctcg gcctcgacgt cggcggccgt caccacgtag ccgtcaccga
      421 agtggcgaaa gtccagcttg acgcccggct gaaagcacca ctccagcggc acctcgtcga
      481 tggtccaggc cggcttcttg ccgcccagcg ccttgtccat ggtgctgtga aaatggtagg
      541 gcgcgtccag gtgcgtgccg ttgtgggtcg acagctgcac catctccacc gcccacacct
      601 cgccgtcggg caggtcggac tgcttgaggc ccggaaagaa cggcgccatc tgctcgaacg
      661 agttctggtg gttgaagtac tggatcttcg gcgcgaaggc cggcgggtcg gagacgacgt
      721 cgttttccag gtagatcgac aggtcgatga acttgcgggt catgaggggc tactccttta
      781 cttccagtga accatgggca ggccggccac gggcgagcgg aaatacggga tgctcgtgcc
      841 catcaggctg ccgatgtaaa cggtgcgcaa gtcgggtccg ccaaaggtga cgctggccat
      901 ccagggcgcc agcgtgccgc gcgcggccat catggcctcg gccgtcacgg tgccggcggc
      961 catgtgcgcc agcagcgcct gcgatgcctg cgggttgccg tcgtccagca gcagcaggcg
     1021 ttcgccctcg ggcgtcagcg cgatcagctg atcgaccatc accagcgtgc accacaggtt
     1081 gccgaaggcg tcgaaggcga tgccgtccgg atagccgccc aggtgcgcgg ggccaaacac
     1141 ctcgcggtcg gtcagcgcca caccctgcgc gccctcgacg acacgcatgc ggctgatgtg
     1201 cgggccggtg gtctcgacga tgtagagcca ctcctcgcgc gcatcgagcc ggatctcgtt
     1261 ggtgaagaaa aagccctcgg ccaccacgcg cagcacgccg cgctcgacca ccgcgatgca
     1321 gccgtcctgc acgcgcgagg cggccgcctg cgtccagggg ttgacgcggg tggacacggt
     1381 gatccagatg cggtccttgc tgtcgcgcag cacgaagttg accttgccga tcggccggcc
     1441 gtcgatgcgg tcatagagca cgcgggtgtc gccaccccgt gtcatcactt ccagccggtc
     1501 ggtgccgaag ttgctgatca ggatgtcgcc gttggccgca aaggccaggc cgttgggcag
     1561 cgtgccggtg gtgaacttgt gctccagctc ggcgctgctg gcggccgcgg cgctgccaaa
     1621 gcgggcgtcg gcgcgctggc cgatgaagcg ctgcgtgcca tcgggcgcga tgcgcatcac
     1681 gccgccgcgc gcatcggccg accacagcgt gccgtccggc tcggccagga tgcattcggg
     1741 gcgctgcagg tcgtggccga tgcggcggat ctgcgcgcgg tcgacggcaa agccgcgcag
     1801 cgggttggcg ggcatggtca tcctccgggg gtcttgagca gcagcagact gagttgcggc
     1861 acgaacacca gcagcagcag caccgccagc atcatcagcg tgaacggcag ggtgccgcgg
     1921 aagatgtcgc ccagcgaaat tcggctgtcg gacagggtgc ttttgatgac gaacaccgac
     1981 aggccgaacg gcggcgtcag caggccgatt tccaccgcca ccacggtcac cacgccgagc
     2041 cagatcaggt tcatgccgaa ctgctcggcg atcggcacca tcagcggcag cacgatcagc
     2101 aggatcgacg acgaatcgat gatcatgccc agcaggatga tgaagccgat gtagatcagc
     2161 agaaagccgg tcggccccag ccccagctgc gtcatccatt cgaccaggaa ctgcggcagc
     2221 cccgacatcg ccagcatgcg cgtgtagatg gtggcgcaga tgatcagaaa gctcaccgac
     2281 accgtgacgt ggccggtctc ggtcagcacc ccccacagcg tgcgccagtc cagcttgcgg
     2341 cgcagcagcg ccaccgccag cgccaccgcc gcgccggtgg cgccggcttc ggtggcggtg
     2401 aaaaagcccg cgtacagccc gcccagcacc gaggcgatca gcagcacgct gggcagcagc
     2461 ttgagcgcca gcgtgcccac cggcagcaac gctgcctcct gcgctggctg cgcctggccg
     2521 ccggcataca ccgagcgcgg ccacagcgtg gccatcagca cgatgcccag cgagtacgcc
     2581 agcgccagca ccagccccgg cacgatgccg gcggtgaaca gatcgcccac cgactgattg
     2641 gccagaaagc cgtacaggat catcagcagg ctgggcggaa tcagcatgcc cagcaccgac
     2701 gagccggcca ccaccccgac ggcaaagcgc ggctggtagc cgaagcgcag catctcgggc
     2761 accgccacgc gcgtgaacac cgcggccgag gcgatcgaga tgccggtgat ggcggcaaac
     2821 accgcgttcg acgccacggt ggccacgccc agcccgccgc gcacccgccg cagcacctgg
     2881 ttggccacct cgaaggcgtc cttgccgacg tcggcgcgcg ccaccagaaa gccggtcagc
     2941 acgaacagcg gcaccacgcc gaagatgtgg ctggccaccg cgtcctggct ggcctgcgcc
     3001 agcaggttgg ccgccacgtc catgtcgccg cggatcagcc acacgccgac gaacgacacc
     3061 agcgccagca ccaccgccac atgcatgccg gcccagatca gccccagcat cgccagcagc
     3121 gacaggcagg ccaccgcgat cgggctcatg gcgcggccct ccgggcggcg cgcggcggcg
     3181 ttcgcgcttg caagcggccc ggcgcgctca tgaccgacct ttcagcccag cgcgcaggcg
     3241 ccgcagatcc agccaggcca gcagcacgaa ggtcagcagg gtcatcacca tgcccaccag
     3301 catgatcagc cgcaccggcc acaccggcgc cgtgaagtcg cccagcgcgc ccacgtactc
     3361 ctggatgcgg atcgactcca ccagcggcgc ccacgccgcc cacaggatca ccgccatcag
     3421 ccccgccccc accaggtggt agagcgcctg cagcagcgcc gccagccagg gcgtggtgcg
     3481 cttgaggcgg tccagcagca cgtcggcgcg tgtcaggcgc ccgctggcca gggtgtcggc
     3541 cagctgcagg aagacgatgc cgacgatcga cagcgacacc agctcggtgg tgccgcgcag
     3601 cggcgcgttc agcagctcgc gccccagcac gtcggcgttg atcagcagca tcagcgccac
     3661 gatccacagc gtgcccagcg cgttgagcgc ctgcgtcagc cgctcgaagc gccccggcag
     3721 cggctgcccg taggcatcgg tcacttcaga tttcatagct gttggcgctt gctggacaag
     3781 cgctggaggc aaaaaacgct tgaaactttc gaacagcccc gggcgcgtgc taccgagccg
     3841 gcctgcttca agcggccgcg cagccggcca tgccagcggc cgtcgtggcc ggggttgccc
     3901 cggcggtagg tatcgatcgc ttcagatttc atagctgctg gcgcttgctg gacaagcgct
     3961 ggaggcataa atcgtttgaa gtcctctccg gcgcccaagc gcacgcgacg cgccgccagc
     4021 ctgattcgag cggccgcgca gccggccatg ccagcggccg ccgtggccgg ggt
//
LOCUS       DAOOOG010000206         4061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_26615_length_4061_cov_34.597853, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000206 DAOOOG010000000
VERSION     DAOOOG010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26615_length_4061_cov_34.597853"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PK242_11990"
     CDS             complement(<1..190)
                     /locus_tag="PK242_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ94781.1"
                     /translation="MDPAVDPDRRWRHLLGKLRFRHLQLLATVHARGSLHAAAAAMHL
                     TQPSLSKALAEVEAAFGFP"
     gene            304..1278
                     /locus_tag="PK242_11995"
     CDS             304..1278
                     /locus_tag="PK242_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOZ94782.1"
                     /translation="MQRRAFLATSAACCLPVWTARAADNFPVRPVHIVVPYAAGGGPD
                     VQTRQLGPLLGEALGQPIVVENKVGAAGVLAAQVVAAAAPDGYTVLQGAITHLLQKVL
                     QPSLKFDPLADFSPIGNISTGASVLVVAADAPWRTAQELIAAARAAPGKMNFGSGGIG
                     TTAHLAGATLVALAKLDAVHVPLRGSVEIAGSLLRGDTQFAFPIAATALPQIQGGKLR
                     ALAITSAERASALPDVPTLHELLGSKLAVQEAWSGLWAPARTPPEVVQRLFAALGSTL
                     KTPAARKIIEAGGGQLAASASPQAYAEFIRAEGAKWTEIVRLIGLTPN"
     gene            1289..2554
                     /locus_tag="PK242_12000"
     CDS             1289..2554
                     /locus_tag="PK242_12000"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOZ94783.1"
                     /translation="MSKPLEGVRVLDLSHVIAGPLASFYLAQLGAEVIKIEAPQGGDV
                     MRAARVRPGDGDTPAGFTALNAGKRSLAIDIRQPQGAELVRRLAATAEVFIENFRPGV
                     VAHCGLDYESIRAVRPDIVYCSISGYGQQGAWAGRGAYDHVMQALTGMMLMSGASADA
                     PPIKVGFPVIDVAVGMLGALSVSAALHRRARTGEGQHIDASMLQASMMLMYPNASSYL
                     TEGVEPQRAGNRGYTGSPTADTYRCADGWLAVAANTPAQFRKLTAALGLQALCEDGEL
                     LDLRAFNAPGGGFVVARDADGLRARFEAAFARQCAQPMEEHLNAEGVPAARVRRLGEF
                     LDEVRTGDKASFAPLTLGPAERPVHTPGLGFCARPASRADGRTAPALGADGQALLHEL
                     GLDATELQALQASGVLRLPAQPPVAGYAA"
     gene            2589..3965
                     /locus_tag="PK242_12005"
     CDS             2589..3965
                     /locus_tag="PK242_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HOZ94784.1"
                     /translation="MWTLICRVLLGLLTLTFTALASAQEVGPARFFDLPAIRDESTLA
                     TTIRSDAVRASHVRPGQRVRVIELEFTSQHWKGLVWKHPARIYVPDGYAGGGNAGIIG
                     TERQFFDRPDWPRRRIPGTELDTEAEYGEATALDLGLPIMLFANPAEDYWGLGESDLA
                     GYTLRQALKTGDLSWNGYHPITVAYLRAITLLHALPGVASQRAVLMGCSKRGQAVGIA
                     TGVDPERVAGVMAACHFGGNTLYYIARKFAEFGAGVGGPDQVRQGPGFQPADEVLRAI
                     NNPIGLQMLVHYDPYMWRHRIRAPFLVALGTNDEFFALGTPNSMLGEMKGDRAFLAID
                     NLRHSWVSTKHLAAWRMWLAHSFLGRPIPGISARAHVDGAELAVQARVEAATDPIKVR
                     LFHAYNPLQTDWRRAHWSAIELPLVDGAYAARLPRREGQRLAWYVEVEDRGAGSTGYF
                     SSLVQIAD"
BASE COUNT          602 a         1480 c         1392 g          587 t
ORIGIN      
        1 gcggaaaacc gaacgcggcc tcgacctcgg ccagcgcctt gctgagcgat ggctgtgtca
       61 ggtgcatggc cgcggcggcc gcgtgcaggc tgccacgcgc atgcacggtc gccagcagct
      121 gcaggtggcg aaaccgcagc ttgcccagca ggtggcgcca tcggcgatcg ggatcgacag
      181 cagggtccat ggtgttggtc agcaattctg tttggctatc aatccataga aatttaccag
      241 ttttacgaat accttgagat ccctagactg accccatcgt cgtcaacccc aggagacccc
      301 gccatgcaac gcagagcttt tcttgccacc agcgccgcct gttgtctgcc cgtctggacc
      361 gcccgtgcgg ccgacaactt cccggtgcgc ccggtgcaca tcgtcgtgcc ctacgccgcc
      421 ggtggcggcc ccgatgtgca gacgcgccag ctcggcccgc tgctgggcga ggcgctgggc
      481 cagccgatcg tggtcgagaa caaggtcggc gccgccgggg tgctggcggc acaggtcgtc
      541 gccgccgccg cgcccgacgg ctacaccgtg ctgcagggtg ccatcacgca tttgctgcag
      601 aaggtgctgc aacccagcct gaaattcgac ccgctggccg acttcagccc gatcggcaac
      661 atttccaccg gcgcctcggt gctggtggtc gcggccgacg ccccgtggcg cacggcgcag
      721 gagctgatcg ccgcggcgcg cgccgcgccc ggcaagatga acttcggctc gggcggcatc
      781 ggcaccacgg cgcacctggc cggtgccacc ctggtggcgc tggccaagct cgacgcggtc
      841 catgtgccgc tgcgcggctc ggtggagatc gccggctctc tgctgcgcgg cgacacccag
      901 ttcgccttcc cgatcgccgc caccgcgctg ccgcaaatcc agggcggcaa gctgcgcgcg
      961 ctggccatca ccagcgcgga acgggcgtca gccctgcccg acgtgcccac gctgcacgag
     1021 ttgctgggca gcaagctggc ggtgcaggag gcctggtccg gcctgtgggc ccccgcccgc
     1081 acgccgcccg aggtggtgca gcgcctgttc gcggcgctgg gcagcacgct caagacgccc
     1141 gcggcacgca agatcatcga ggccggtggc ggccagctgg cggccagtgc cagtccgcag
     1201 gcctatgccg aattcatccg cgccgaaggc gccaagtgga ccgagatcgt gcgcctgatc
     1261 ggcctgaccc cgaactgagg agccgcacat gagcaaaccc ctggaaggcg tgcgcgtgct
     1321 cgacctgtcg cacgtcatcg ccgggccgct ggcctcgttc tacctggcgc aactgggcgc
     1381 cgaggtgatc aagatcgaag cgccgcaagg cggcgacgtg atgcgggcgg cgcgcgtgcg
     1441 tcccggcgat ggcgacacgc cggcgggctt cacggccctg aacgccggca agcgttcgct
     1501 ggccatcgac attcgccagc cgcaaggcgc cgagctggtg cgccggctgg cggccacggc
     1561 cgaggtgttc atcgagaatt ttcgccccgg cgtggtggcg cactgcgggc tggactacga
     1621 gtccatccgc gccgtgcggc ccgatatcgt ctactgctcg atctcgggct acggccagca
     1681 gggtgcgtgg gccggccgcg gcgcctacga ccacgtgatg caggcgctca ccggcatgat
     1741 gctgatgtcc ggtgccagcg ccgacgcgcc gccgatcaag gtcggctttc cggtcatcga
     1801 cgtggcggtc gggatgctgg gcgcgctgtc ggtcagcgcc gcgctgcacc ggcgcgcacg
     1861 gacgggcgag ggccagcaca tcgacgcctc gatgctgcag gcctcgatga tgctgatgta
     1921 ccccaacgcc agcagctacc tgaccgaagg cgtcgagccg cagcgcgcgg gcaaccgcgg
     1981 ctacaccggc agccccacgg ccgacaccta ccgctgcgcc gacggctggc tggccgtggc
     2041 cgccaacacc ccggcgcagt ttcgcaagct caccgccgcg ctcggcctgc aggcgctgtg
     2101 cgaggacggc gagctgcttg atctgcgcgc cttcaacgcg cccggcggcg gcttcgtggt
     2161 ggcgcgcgac gccgacggcc tgcgcgcgcg cttcgaggcg gcctttgccc gccagtgcgc
     2221 gcagccgatg gaagaacacc tgaacgccga aggcgtgccg gcggcgcggg tgcgccggct
     2281 gggcgagttc ctggacgagg tacgcaccgg cgacaaggcc agcttcgcgc ccctgaccct
     2341 gggcccggcc gagcgtccgg tgcacacccc cggcctgggt ttctgcgccc ggccggccag
     2401 ccgcgccgac ggccgcaccg cgcccgccct gggcgccgac ggccaggccc tgctacacga
     2461 gctgggcctg gatgcaaccg agctgcaggc gctgcaggcc agcggcgtgc tgcgcctacc
     2521 cgcgcagccg ccggtcgccg gctatgccgc ctgaaatccc accgccccct cacgcgagaa
     2581 cccgaaccat gtggaccttg atctgccgcg tcctgctcgg cctgctcacc ctcacattca
     2641 ccgccctggc gtcggcacag gaggtcggcc cggcgcgctt cttcgacctg cccgccatcc
     2701 gcgacgaaag cacgctggcc accacgatcc gcagcgacgc cgtgcgcgcc agccacgtgc
     2761 gcccgggcca gcgcgtgcgc gtgatcgagc tggaattcac cagccagcac tggaagggcc
     2821 tggtgtggaa gcacccggcg cgcatctacg tgccggacgg ctacgcgggc ggcggcaacg
     2881 ccggcatcat cggcaccgag cgccagttct tcgaccggcc cgattggccg cgccgacgca
     2941 tccccggcac cgagctggac accgaagctg aatacggaga ggccacggcg ctggacctgg
     3001 ggctgccgat catgctgttt gccaacccgg ccgaggacta ctggggcctg ggcgaatccg
     3061 acctggccgg ctacaccctg cggcaggcgc tcaagaccgg cgacctgagc tggaacggct
     3121 accacccgat caccgtggcc tatctgcgcg ccatcaccct gctgcatgcg ctgcccggcg
     3181 tggccagcca gcgggcggtg ctgatgggct gctccaagcg cggccaggcg gtgggcatcg
     3241 ccaccggggt cgatcccgag cgcgtggccg gcgtcatggc ggcctgccat ttcggcggca
     3301 acacgctgta ctacatcgcg cgcaagttcg ccgagttcgg cgccggcgtg ggtgggcccg
     3361 accaggtgcg ccagggcccg ggctttcagc ccgccgacga ggtgctgcgc gccatcaaca
     3421 accccatcgg cttgcagatg ctggtgcact acgaccctta catgtggcgc caccgcatcc
     3481 gcgctccttt tctggtcgcc ctgggcacca atgacgagtt cttcgccctg ggcacgccca
     3541 acagcatgct cggcgaaatg aagggcgacc gggcgttcct ggccatcgac aacctgcgcc
     3601 acagctgggt gtcgaccaag catctggcgg cctggcgcat gtggctggcg cacagcttcc
     3661 tgggacgccc catcccgggc atctcggcgc gcgcccacgt cgatggcgcg gagctggcgg
     3721 tgcaggcgcg cgtcgaagcc gccaccgacc ccatcaaggt acggcttttc cacgcctaca
     3781 acccgctgca gaccgattgg cgccgggcgc actggagcgc gatcgagctg ccgctggtcg
     3841 atggcgccta tgcggcccgc ctgccgcggc gcgaaggcca gcgcctggcc tggtacgtcg
     3901 aggtggaaga tcggggtgcg ggcagcacgg gctacttcag ctcactggtg cagatcgccg
     3961 attgaccggc cagatgaacc gcccggagca cggcagcaag tctagttgta gtttctcacc
     4021 acgttgttcc cctcgaagcc gaggcgagtg atgggcggct t
//
LOCUS       DAOOOG010000207         3988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_27292_length_3988_cov_29.018561, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000207 DAOOOG010000000
VERSION     DAOOOG010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27292_length_3988_cov_29.018561"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PK242_12010"
     rRNA            complement(<1..323)
                     /locus_tag="PK242_12010"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            978..2888
                     /locus_tag="PK242_12015"
     CDS             978..2888
                     /locus_tag="PK242_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ94785.1"
                     /translation="MALLTLLDAQLAFGHLPLLDHADFALETKERVGLIGRNGAGKSS
                     LLRILGRLERVDDGQLQVARDLTVAYVAQEPALAADATVADTVAEGLAPLRALIEAWG
                     AGADDLDALQARIEAQDGWGWRQRVDEVLQRLRLPADAPVGTLSGGARKRVALAQALV
                     TRPDVLLLDEPTNHLDLDAIQWLEELLLQFAGSLVVVTHDRAFLDRVATRIVELDRGR
                     LRSYPGNFARYQSLKAEQLAQEASVDARADKLLAQEEAWIRQGVEARRTRAQGRVRRL
                     QALRAARAARRTSVGRVRLDIDADVAGYQGKLVAELRDVRLAFGEQVIVEGLSTTVLR
                     GDKIGLIGPNGAGKTTLLKLILGQLAPDRGQVRHGANLQVAYFDQMRAALDPEATLED
                     FISPGSEWIEIGNRRQHVKSYLGDFLFSPARAAAPVRSLSGGERARLLLARLFARPAN
                     VLVLDEPTNDLDIDTLELLEELLQDYPGTVFLVSHDRTFLDHVVTSTLAWDGPGRWRE
                     YEGGVQDWLTQSARWQTLRAPEPPAAAQATTAVSNPMGLKGPPSERQQLQKKKLSYKA
                     QRELDELPARIEALEAEQAALRAALADGALYQSDLARAIALQARDSAIDDELTAALER
                     WAELEAAGQRPA"
     gene            2956..3654
                     /locus_tag="PK242_12020"
     CDS             2956..3654
                     /locus_tag="PK242_12020"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HOZ94786.1"
                     /translation="MNHPWLWMVAAAALLAALLAAFWLLRRRGGAEQPLGPDDRFAPV
                     AALSPAEMALLDYLVRAFPGRPVLFRVPLARLLTVRRAGNRLAAQRRLAALAVDYLAC
                     SRDGKPVYAFELDAVHGDDAQAEQDAREKHRALKTAGVRLIRLKRSTRHLPPPDEFRR
                     QLRAAALPAQEASGDAAATADPWQGAERAHPTPARSANAPREAGDTIPMSMTDLMGLP
                     PVVDDDADRPPARR"
BASE COUNT          626 a         1338 c         1341 g          683 t
ORIGIN      
        1 cgtgtctcag tcccagtgtg gctgatcatc ctctcagacc agctacagat cgttggcttg
       61 gtaggctttt atcccaccaa ctacctaatc tgccatcagc cgctccagac gcgcgaggtc
      121 ttgcgatccc ccgctttcat ccgtagatcg tatgcggtat tagctactct ttcgagtagt
      181 tatcccccac gtctgggcac gttccgatgt attactcacc cgttcgccgc tcgccgccag
      241 gttgccccgc gctgccgctc gacttgcatg tgtaaagcat tccgccagcg ttcaatctga
      301 gccaggatca aactctacag ttcgatcttg aaatttatac tctttcgagt ttactcattt
      361 aaacggaatt gaagtgaact tcacttctgt tctcatgagc gtttgatagt ctgaaagacc
      421 ttggcaattc gccatcaaac gcccacgctt atcggctgta tgtttttaat gaaccggcgc
      481 cagacctgct gtcctgcgct gcctcagttg cgatcaactg agccttgaat tatgacctgt
      541 tttcgagaaa cctgtcaatc ttcagggttt ttacctagag cgctcaattt tttgaactcg
      601 ctccgggcca agcccttcca gctgttaact ggtgagcctt gaattatggc atgttgttta
      661 acatcccgtc aaggcctggg gtctttcgtt gaaggctggc accatttggc gaccttccct
      721 ctgacccctt ccgcgccgat cgtcggccgc ttgcgcatgg ccgccgctct ggcgtggtcc
      781 gcttgcttct gccagctggc atcgatcagc gaagccatag actatagcac tgttttttgg
      841 atctgcgcaa ggcgcggcaa aaatttttct gcccaggtgt cggacgagat cgtgcgaggc
      901 ttgtggccca ctgtctcatg ccggctcgca cgcgctgccg gatccgctca catcgaaccc
      961 tccatcctca gacgttcatg gccctgctca ccctgctcga tgctcaactg gcgttcggcc
     1021 acttgccgct gctggaccac gcggatttcg cgctcgagac gaaagagcgg gtggggctga
     1081 tcgggcgcaa cggcgccggc aagtcttccc tgctgcgcat tctggggcga ctcgagcgcg
     1141 tggatgacgg ccagctgcag gtggcgcgcg atctgacggt ggcctacgtc gcccaggagc
     1201 cggcgctggc ggccgacgcc acggtggccg atacggtcgc cgagggactg gcgccgctgc
     1261 gcgccctgat cgaagcctgg ggcgcaggtg ccgatgatct ggacgcactg caggcgcgca
     1321 tcgaggcgca ggacggctgg ggctggcgcc agcgggtgga cgaggtgctg cagcgcttgc
     1381 ggctgcccgc cgacgcgccg gtgggcacgc tgtcgggcgg cgcgcgcaag cgcgtggcgc
     1441 tggcgcaggc gctggtgacg cggcccgacg tgctgctgct ggacgagccg accaaccacc
     1501 tcgacctgga cgccatccag tggctggagg agttgctgtt gcagtttgcc ggcagcctgg
     1561 tggtggtgac gcacgaccgc gcgtttctgg accgcgtggc cacgcgcatc gtcgagctgg
     1621 atcgggggcg gctgcggtcc tacccgggca acttcgcgcg ctaccagtcg ctcaaggccg
     1681 agcagctggc ccaggaggcg agcgtcgacg cccgtgccga caagctgctg gcgcaggagg
     1741 aagcgtggat ccggcagggt gtggaagcgc gccgcacgcg cgcgcagggc cgggtccggc
     1801 ggctgcaggc cctgcgggcg gcgcgcgcgg cacggcgcac gtcggtgggc cgggtgcggc
     1861 tggacatcga cgccgacgtc gcgggctacc agggcaagct ggtggccgag ctgcgcgacg
     1921 tgcgcctggc cttcggcgag caggtgatcg tcgaaggcct cagcaccacc gtgctgcgcg
     1981 gcgacaaaat cggcctgatc gggcccaacg gcgccggcaa gaccaccctg ctcaagctga
     2041 ttctgggcca gctggcgccc gaccgcggcc aggtgcggca cggcgccaac ctgcaggtgg
     2101 cgtatttcga tcagatgcgc gccgcgctcg atcccgaagc cacgctggag gacttcatca
     2161 gcccgggcag cgaatggatc gagatcggca accggcgcca gcacgtcaag agttacctgg
     2221 gcgacttcct gttcagcccg gcgcgcgccg ccgcgccggt gcgcagcctg tcgggcggcg
     2281 agcgggcccg gctgctgctg gcgcgcctgt tcgcgcgccc ggccaacgtg ctggtgctgg
     2341 acgagccgac caacgacctg gacatcgaca cgctggagct gctggaggag ctgctgcagg
     2401 actacccggg cacggtgttt ctggtcagcc acgaccgcac cttcctcgac cacgtggtga
     2461 ccagcaccct ggcctgggac ggcccgggcc gctggcgcga atacgaaggc ggggtgcagg
     2521 actggctgac gcagagcgcg cgctggcaga cgctgcgggc accggaaccc ccggcagcgg
     2581 cccaggcaac gacggcagtc agcaacccaa tgggtctcaa aggtccgccc agcgagcgcc
     2641 agcagctaca aaaaaagaag ctatcgtaca aggcgcagcg cgagctcgac gagctgccgg
     2701 cccgcatcga ggcgctggag gccgaacagg ccgcgctgcg cgccgcgctg gccgacggcg
     2761 cgctctacca gagcgacctg gcgcgcgcga tcgccctgca ggcgcgcgac agcgccatcg
     2821 acgacgagct gaccgcggcg ctggagcgct gggccgagct cgaagcggcc ggccagcgcc
     2881 cggcctgacg caggtcgcta cagtgtggtc acgcggcccg tccaggtgcc gccccgatga
     2941 ctggggagtg cggacatgaa ccacccatgg ctgtggatgg tggcggcggc ggcgctgctg
     3001 gcggcgctgc tggccgcctt ctggctgctg cgtcggcgcg gcggtgccga gcagcccctt
     3061 ggtccggacg accgcttcgc gccggtggcg gcgctgtcgc cggccgagat ggcgctgctg
     3121 gactatctgg tgcgagcctt tcccggccgg ccggtgctgt ttcgggtgcc gctggcgcgc
     3181 ctgctcacgg tgcggcgtgc cggcaaccgc ctggccgccc agcggcgcct ggccgcgctg
     3241 gcggtcgact acctggcctg cagccgcgac ggcaaacccg tctacgcctt cgagctcgac
     3301 gccgtgcacg gtgacgatgc ccaggccgag caggacgctc gcgagaagca ccgcgcgctc
     3361 aagaccgccg gcgtgcgcct gatccggctc aagcgcagca cgcgccacct gccgccgccc
     3421 gacgaattcc ggcgccagtt gcgcgccgcc gccctgccgg cacaggaagc gtcgggcgac
     3481 gccgccgcga ccgccgaccc gtggcagggt gccgagcgcg cccaccccac gcccgcgcgg
     3541 tcggccaacg ctccgcgcga ggcgggcgac accatcccga tgtcgatgac cgacctgatg
     3601 ggcctgccac cggtggtcga cgacgacgcc gaccggccgc cggcgcgccg ctgagccttt
     3661 ggtttcggcc tctggatgtc ttgacatttc aacccaggag gctgtcgggc ttggagcgcc
     3721 gaaagcgctc ttgaagagcg gcgtagccaa aatcggcccc agcgagacca gtttttgccg
     3781 gatttgcagc ccaatagcta agctattggg caaaaagccg gtgctctcga agagcggcga
     3841 agccaaaaat gggccgctgc ggtcgatttg cagccggcga tttccaagtt cgacgacctc
     3901 ctagtgttca agattccgaa gccatgggct gagctcagcc gccaccttgc ggcttggccg
     3961 ggcgctgcca gttggcgatg tgggcctg
//
LOCUS       DAOOOG010000208         3937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_27774_length_3937_cov_40.713035, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000208 DAOOOG010000000
VERSION     DAOOOG010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27774_length_3937_cov_40.713035"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PK242_12025"
     CDS             <1..359
                     /locus_tag="PK242_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ94787.1"
                     /translation="SRDPAAVQAYQADPLVHDRISARLAHFIATAGPAVLQRAPRWKL
                     PTLLLYAGQDRAVAPAGSAAFARAAPAEWVTAHAFPLHYHELFNDLDSAEVFQVLHDW
                     LTARFAPVALGRAAPF"
     gene            complement(388..1563)
                     /locus_tag="PK242_12030"
     CDS             complement(388..1563)
                     /locus_tag="PK242_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ94788.1"
                     /translation="MNRHLLLLTLVQGLFLVNNVTFIAINGLVGLQLAPYGWMATLPV
                     MGYVVGGALSAPLVARTQARFGRKGSFQIGLAVALGSALLAAHAAQSRQFWLLLAATV
                     IAGYYNANGQLYRFAAAELVAPDWREKAVSLVLAGGLLGAVVGPNLAAWTRTVLPTPF
                     VGAYLALALVALLAMAVLALIRFPAAPARAAGADTGRPLGQIMRQPAFIVATVGAALG
                     YGVMNLLMAATPLAMQVCGFEFGATAQVLQWHVIGMYAPGFFTGHLIRRFGALAVMGA
                     GVVLNLLCVAVALTGVELTQFLAALTLLGVGWNFLFTGSTTLALTAYRPEEKDRAQAA
                     INFWVFATMAFTSFASGALVTTQGWSWLNLGSLLPLLVTGAALAWLGLRRRAAATGA"
     gene            complement(1630..2478)
                     /locus_tag="PK242_12035"
     CDS             complement(1630..2478)
                     /locus_tag="PK242_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HOZ94789.1"
                     /translation="MLFNGFYINLDESPQRHHAMQARLKALGLDGMTRRFAGQRGDGR
                     AARISASQLGCFLSHQAVLEQAPADRFTLVLEDDAGLAAALPRRLPALLDPAQAQPWD
                     ILFLGQTVPYDSIHPLDRLIKASKELGDIGAPDFARFFLMDGAMHYVWGAFGYVVHPR
                     ALPRLRALLRQFAAHGYPTSWDGTLATLIKQGQLTARCLFPYLVGVDTGHDSTISGRG
                     PHLQHQLHALLVNLFLADADEQQRLRAQALAFCPSATDWQAAYVSWLTYRKLSAPPDS
                     GTSDFT"
     gene            complement(2516..3232)
                     /locus_tag="PK242_12040"
     CDS             complement(2516..3232)
                     /locus_tag="PK242_12040"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOZ94790.1"
                     /translation="MPRFHVCLSLAPGARLLLPPGAARHAQVLRLQPGSDITLFNGEG
                     GEWTAQITRMGRSDVEVAVQAHHAVEREAARAVHLAVGLMAAERMDWLVEKATELGAA
                     SVTPVLMARSSLRLAGERAARKRAHWQAVAIAACEQSGRNRLLDMREQLTFQEYLRQA
                     PDGLRWLLSLDPAAPALGERVAAQAMARPVALLSGPEGGLDPTEEAAARLAGFAPVSL
                     GPRVLRAETAPLAALALLLA"
     gene            3300..>3937
                     /locus_tag="PK242_12045"
     CDS             3300..>3937
                     /locus_tag="PK242_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOZ94791.1"
                     /translation="MAPSSAAVTPCPSCRAPMQAQQVAAHDGTTVTLDLCHACHGIWF
                     DPRENQRMAPEGVLGLFRHLHEHREDAQAPLARRLSCPRCRDPLAEGSDVVRSGRYFT
                     WRCPRRHGRFATFSSFMVEKGFVRQLTELEITALAERTRIIHCGSCGAPFDLRQDRAC
                     PYCRAAPSLLDPQAVEQALDGYDAKARRLAEGPRAPDLAEALITLERDRERAR"
BASE COUNT          582 a         1429 c         1425 g          501 t
ORIGIN      
        1 tgtcgcgcga cccggcggcg gtgcaggcct accaggccga cccgctggtg cacgaccgca
       61 tcagcgcccg gctggcgcac ttcatcgcca ccgccggccc ggccgtgctg cagcgcgcgc
      121 cgcgctggaa gctgcccacg ctgctgctgt acgccggcca ggaccgcgcg gtggcgccgg
      181 ccggcagcgc agccttcgcc cgcgccgcgc ccgccgaatg ggtgacggcg cacgcctttc
      241 cactgcacta ccacgagctg ttcaacgacc tcgacagcgc cgaggtgttc caggtgctgc
      301 acgactggct gacggcgcgc ttcgccccgg tggccctggg gcgcgcggcg ccgttctgag
      361 gcgccggggc cggcccggtg tcgggcttca ggcgccggtg gcggcagcgc gccggcgcag
      421 gcccagccag gccagcgcgg cgccggtgac cagcagcggc agcagcgagc ccaggttcag
      481 ccagctccag ccctgggtgg tcaccagcgc cccggaggcg aacgaggtga aggccatggt
      541 ggcgaacacc cagaagttga tggccgcctg cgcgcggtcc ttttcctccg ggcggtaggc
      601 cgtcagcgcc agcgtggtgc tgccggtgaa caggaagttc cagcccacgc ccagcagcgt
      661 cagcgcggcc aggaactgcg tcagctccac cccggtcagc gccaccgcca cgcacagcag
      721 gttcagcacc acgccggccc ccatcaccgc cagcgcgccg aagcgccgga tcaggtggcc
      781 ggtgaagaag cccggcgcgt acatgccgat cacgtgccat tgcagcacct gcgccgtggc
      841 gccgaactcg aagccgcaca cctgcatggc cagcggcgtg gcggccatca gcaggttcat
      901 cacgccatag cccagcgccg cgcccacggt ggcgacgatg aaggccggct ggcgcatgat
      961 ctgccccagc ggccggccgg tgtcggcccc ggcggccctg gccggcgcgg ccgggaagcg
     1021 gatcagcgcc agcaccgcca tggccagcag cgccaccagc gccagcgcca ggtaggcgcc
     1081 gacgaagggc gtgggcagca cggtgcgcgt ccaggccgcc aggttggggc cgaccaccgc
     1141 gcccagcagg ccgccggcca gcaccagcga cacggctttt tcgcgccagt cgggcgccac
     1201 cagttcggcg gcggcaaagc ggtacagctg gccgttggcg ttgtagtagc cggcgatcac
     1261 cgtggccgcc agcagcagcc agaactgccg cgactgcgcc gcatgggccg ccagcagcgc
     1321 cgagcccagc gccaccgcca gcccgatctg aaacgacccc ttgcggccaa agcgcgcctg
     1381 cgtgcgcgcc accagcggcg ccgacagcgc gccgcccacc acgtagccca tcaccggcag
     1441 cgtcgccatc cagccatagg gcgccagttg cagccccacc agcccgttga tggcgatgaa
     1501 ggtgacgttg ttgaccagga acaggccctg gaccagggtg agcagcagca ggtggcggtt
     1561 catgtggtca tccggatact attgaaacag gagcttgcgc cgcttgaccc tggcggcctg
     1621 gcaagggttt caggtgaaat cactggtgcc cgagtcgggt ggcgccgaca gcttgcggta
     1681 ggtcagccag ctgacgtagg cggcctgcca gtcggtggcc gaggggcaga aggccagggc
     1741 ctgcgcgcgc agccgctgct gttcgtcggc gtcggccagg aacaggttga ccagcagggc
     1801 gtgcagctgg tgctgcagat gggggccgcg gccgctgatg gtgctgtcgt gcccggtgtc
     1861 gacgccgacc agatacggga acaggcagcg ggccgtcagc tggccctgct tgatcagcgt
     1921 ggccagggtg ccgtcccacg aggtcgggta gccgtgggcc gcgaactgcc gcagcaaggc
     1981 gcgcagccgc ggcagcgccc gggggtggac gacgtagccg aacgcgcccc agacatagtg
     2041 catcgccccg tccatcagga aaaagcgcgc aaagtcgggc gcgccgatgt cgcccagctc
     2101 cttgctggct ttgatcaggc ggtccagagg gtgaatgctg tcgtagggca cggtctggcc
     2161 aaggaacagg atgtcccagg gctgcgcctg tgccgggtcc agcagcgccg gcaggcgccg
     2221 cggcagggcg gcggccaggc cggcgtcgtc ttcgagcacc agggtgaagc ggtccgcggg
     2281 ggcttgctcc agcaccgcct ggtgcgacag aaagcagccc agttgcgagg ccgagatgcg
     2341 ggccgcgcgg ccatcgccgc gctgcccggc aaaacgccgg gtcatgccgt cgagccccag
     2401 cgccttcagg cgcgcctgca tggcgtgatg gcgctgcggc gattcgtcca ggttgatgta
     2461 gaagccgttg aaaagcatgg tgaacgattc tgggcgaaat cccctgggcg ggtgctcacg
     2521 cgagcagcag cgccagtgcc gccagcggcg cggtttcggc gcgcagcacg cgcgggccga
     2581 gactgaccgg ggcaaagccg gccaggcgcg cggcggcttc ttcggtgggg tccagtccgc
     2641 cttcggggcc ggacagcagg gcaaccgggc gtgccatggc ttgcgccgcg acgcgctcgc
     2701 ccagggcagg ggccgccggg tcgagcgaca gcagccagcg caggccgtcc ggcgcctgcc
     2761 ttagatactc ctgaaatgtg agctgttcgc gcatgtccag caagcgattg cggccacttt
     2821 gctcgcaagc ggcaatggcc acggcctgcc agtgggcgcg cttcctggcg gcgcgctcgc
     2881 cggccaggcg caggctgctg cgcgccatca ggacgggcgt cacgctggcg gcgcccagct
     2941 cggtggcttt ctcgaccagc cagtccatgc gctcggccgc catcaggccc acggccaggt
     3001 gcacggcgcg cgcggcctcg cgctccaccg cgtggtgcgc ctgcaccgcc acttccacgt
     3061 cgctgcgccc catgcgggtg atctgtgccg tccactcgcc accttcgccg ttgaacagcg
     3121 tgatgtcgct gcccggctgc aggcgcagca cctgggcgtg gcgcgcggcg ccgggcggca
     3181 gcaacaggcg ggcgcccggg gccagggaca ggcaaacgtg aaatcttggc atcgcgggcg
     3241 gatgaaaaag cagccgccga tggtacgccg gccgccgcca tgccgtagca tcggcgccca
     3301 tggccccgtc gtccgctgcc gtcacgccct gcccgtcctg ccgcgcgccc atgcaggcgc
     3361 agcaggtggc cgctcatgac ggcaccacgg taacgctgga cctgtgccat gcctgccacg
     3421 gcatctggtt cgacccgcgg gagaaccagc gcatggcgcc cgagggcgtg ctgggcctgt
     3481 ttcgccacct gcacgagcac cgcgaggacg cgcaggcccc gctggcgcgg cgcctgagct
     3541 gcccgcgctg ccgcgacccg ctggccgaag gcagcgacgt ggtgcgcagc gggcgctact
     3601 tcacctggcg ctgtccgcga cggcacggcc gctttgccac cttctcgtcg ttcatggtcg
     3661 agaagggctt cgtgcgccag ctgaccgagc tggaaatcac cgcgctggcc gagcgcacgc
     3721 gcatcatcca ctgcggcagc tgcggcgcgc cgttcgacct gcgccaggac cgcgcctgcc
     3781 cctactgccg cgcggcgccg tcgctgctgg acccgcaggc ggtggaacag gcgctggacg
     3841 gctatgacgc caaggcccgg cgcctggccg agggcccgcg cgcgcccgac ctggccgagg
     3901 cgctgatcac gctggagcgc gaccgcgagc gcgcccg
//
LOCUS       DAOOOG010000209         3917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_27974_length_3917_cov_25.729933, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000209 DAOOOG010000000
VERSION     DAOOOG010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27974_length_3917_cov_25.729933"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1304)
                     /locus_tag="PK242_12050"
     CDS             complement(123..1304)
                     /locus_tag="PK242_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOZ94792.1"
                     /translation="MSKTKTPFSWQDPFALHDQLSADERAVQDAAHAYCQDKLLPRVQ
                     QAFRSEQTDPAIFREMGELGLLGPTIPEQYGGAGLGYVSYGLIAREVERVDSGYRSMM
                     SVQSSLVMVPINEFGNEATRQKYLPKLARGEWIGCFGLTEPNHGSDPGSMVTRAKKVP
                     GGYSLSGSKMWISNSPIADVFVVWAKDDEGAIRGFVLEKGWKGLSAPAIHGKVGLRAS
                     ITGEVVMDEVFCPEENAFPEVRGLKGPFTCLNSARFGIAWGALGAAEDCYARARQYVI
                     DRKQFGKPLAANQLIQKKLADMLTEISLGLQACLRLGRMKDEGHPPVELTSIIKRNSC
                     GKALDIARQARDMMGGNGISDEFGVARHLVNLEVVNTYEGTHDIHALILGRAITGMAA
                     F"
     gene            complement(1343..1741)
                     /locus_tag="PK242_12055"
     CDS             complement(1343..1741)
                     /locus_tag="PK242_12055"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOZ94793.1"
                     /translation="MNPQRIHPEGWAPAKGYANGVAARGTQIYVGGQIGWNAQQQFES
                     DDFIAQVHQALRNVAAVLQAAGAGPEHMVRMTWYVLDRDEYNRRLKELGGVYRDVMGR
                     HFPAMSCVQVAGLMEARAKVEIEVTAVIPD"
     gene            complement(1738..3360)
                     /locus_tag="PK242_12060"
     CDS             complement(1738..3360)
                     /locus_tag="PK242_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ94794.1"
                     /translation="MSVPSAQTDRYVHDRLPPREQWPEFRYDLPELRIPDQANLVTVL
                     FDAARANGWSNRLLLRSPGQTLTYAETEAQVARIAEMLIRDFKLEPGNRVLLRGGNSI
                     GMALAWLAVVKAGLIVVPTMPLLRALELGKVIDKAQPALALCEVDLLEDMVLAAQDAR
                     GRLPILAFGDSGRPDGLEQRAARRSPDHAGCATAADDIALLAFTSGTTGVPKAVVTTH
                     RDILAACNTWPRHVLKMTPDDIVAGSPPLAFTFGTGGLLIYPMWAGASVYYPDVRFSP
                     ETMVRKMRDNGITVCYTAPTFYRQMAPYAKALGIPSLRICVSAGEALPDATRQLWKQA
                     TGIEMLDGIGGTEMWHIYISAAGADVRPGTIGKVVPGYQARVVDEHDQEVPRGTVGRL
                     AVIGPTGCRYMDDARQTRYVAHGWNHPGDACIQDEDGYFHYQARDDDMIVTAGYNVGG
                     PEVEDCLLTHPAVAECGVIGKPDAVRGMIIKAFVVLRPGQPDNEAMVEALQAHVRQHI
                     AAYKYPREIQFVTALPRTDTGKLQRFKLRELA"
     gene            complement(3363..>3917)
                     /locus_tag="PK242_12065"
     CDS             complement(3363..>3917)
                     /locus_tag="PK242_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOZ94795.1"
                     /translation="PVMAPHPLATLVFERCRVAGEQMLGELNGGFKLAMQTLDIFRAS
                     VAGAALGMARRALAEAIAHARQRRMFGQTLADFQLTQAKLGEMAALIDGAALLTYRAA
                     WLRDTGQGSPKERSVAAAMAKMTATENAQRVIDMALQMHGGMGVMVGRKVESLYRDIR
                     ALRIYEGATEVQQLIIGKSVLQEA"
BASE COUNT          551 a         1325 c         1382 g          659 t
ORIGIN      
        1 tgggccctgg cgtgaaggtg ctggccgtgg ccagcagcag cagggcactg aattcggcgg
       61 tcgtcacggc aggcggcgca caggcgaacc cgacatgggc gcggcggcgc ggcccatggc
      121 gctcagaacg ccgccatgcc ggtgatggcg cggcccagga tcagcgcgtg gatgtcgtgc
      181 gtgccctcgt aggtgttgac cacctccagg ttcaccaggt ggcgcgccac gccgaactcg
      241 tcgctgatgc cgttgccgcc catcatgtcg cgcgcctggc gggcgatgtc gagtgccttg
      301 ccgcagctgt tgcgcttgat gatggaggtc agctccaccg gcgggtggcc ctcgtccttc
      361 atgcggccta ggcgcaggca ggcctgcaga ccgaggctga tctcggtcag catgtcggcc
      421 agctttttct ggatcagctg gttggccgcc agcggcttgc cgaactgctt gcggtcgatc
      481 acgtactggc gcgcgcgggc gtagcagtcc tcggccgcgc ccagcgcgcc ccaggcaatg
      541 ccgaagcgcg ccgagttgag gcaggtgaac gggcccttca ggccgcgcac ctcggggaag
      601 gcgttttcct cggggcagaa cacctcgtcc atcaccacct cgccggtgat gctggcgcgc
      661 aggccgacct tgccgtgaat cgccggcgcg gacaggccct tccagccttt ctcgagcacg
      721 aagccgcgga tcgcgccctc gtcgtccttg gcccagacca caaacacgtc ggcgatcggg
      781 ctgttgctga tccacatctt gctgcccgac agcgaatagc cgccgggcac cttcttggcg
      841 cgcgtgacca tgctgccggg gtcggagccg tggttgggct cggtcaggcc aaagcagccg
      901 atccattcgc cgcgcgccag cttgggcagg tatttctgcc tggtggcctc gttgccgaat
      961 tcgttgatcg gcaccatgac gagcgaagac tgcacgctca tcatcgagcg gtagcccgag
     1021 tccacgcgct cgacctcgcg cgcgatcagg ccgtagctga cgtagcccag gccggcgccg
     1081 ccgtattgct cggggatggt ggggcccagc aggcccagtt cgcccatttc acgaaagatc
     1141 gccgggtcgg tctgctcgct gcggaaggcc tgctgcacgc gcggcagcag cttgtcctgg
     1201 caataggcgt gggcggcgtc ctgcaccgcg cgctcgtcgg cgctgagctg atcgtgcagg
     1261 gcaaacgggt cttgccagct gaagggggtc ttggttttgg acatggcggg ttccacgggg
     1321 taaggcggat gaggattgtc catcagtcgg gaatgaccgc cgtgacttcg atctcgacct
     1381 tggcgcgcgc ctccatcagc cccgccacct gcacgcagct catggccgga aagtgcctgc
     1441 ccatgacgtc gcggtaaacg ccgcccagtt ccttcagccg gcggttgtat tcgtcgcggt
     1501 cgagcacgta ccaggtcatg cgcaccatgt gctcggggcc ggcgccggcg gcttgcagca
     1561 ccgcggcgac attgcgcaag gcctggtgca cctgcgcgat gaagtcgtcg ctctcgaact
     1621 gctgctgcgc gttccagccg atctgaccgc cgacatagat ctgcgtgccg cgcgcggcca
     1681 cgccgttggc gtagcccttg gccggcgccc agccctcggg gtggatccgt tgcgggttca
     1741 tgccagctcc ctcagcttga agcgctgcag cttgccggtg tcggtgcgtg gcagcgccgt
     1801 cacgaactgg atttcgcgcg ggtacttgta ggctgcgatg tgctgccgca catgcgcctg
     1861 cagggcctcg accatggcct cgttgtccgg ctggccgggc ctgagcacca cgaaggcctt
     1921 gatgatcatg ccgcgcacgg cgtcgggctt gccgatcacg ccgcactcgg ccaccgccgg
     1981 gtgggtcagc aggcagtcct cgacctcggg gccgccgacg ttgtagccgg cggtgacgat
     2041 catgtcgtcg tcgcgcgcct ggtagtggaa gtagccgtcc tcgtcctgga tgcaggcgtc
     2101 gccggggtgg ttccagccat gggcgacgta gcgggtctgg cgcgcatcgt ccatgtagcg
     2161 gcagccggtg gggccgatca cggccagccg ccccacggtg ccgcgtggca cttcctggtc
     2221 gtgctcgtcc accacgcgcg cctgatagcc cggcaccacc ttgccgatcg tgcccgggcg
     2281 cacgtcggcg cccgccgcgg agatgtagat gtgccacatc tcggtgccgc cgatgccgtc
     2341 gagcatctcg atgccagtgg cctgcttcca cagctggcgc gtggcgtcgg gcagggcctc
     2401 gccggcgctg acgcagatgc gcaggctggg aatgcccagc gccttggcat agggcgccat
     2461 ctggcggtag aaggtgggcg cggtgtagca cacggtgatg ccgttgtcgc gcatcttgcg
     2521 caccatggtc tcggggctga agcgcacgtc ggggtagtac accgaggcgc ctgcccacat
     2581 gggatagatc aacagccccc cggtgccgaa ggtgaaggcc agcggcggcg agccggcgac
     2641 gatgtcgtcg ggcgtcatct tcagcacgtg gcgcggccag gtgttgcagg cggccaggat
     2701 gtcgcggtgc gtggtgacca ccgccttggg cacgccggtg gtgccgctgg tgaaggccag
     2761 cagggcaatg tcgtcggcgg cggtggcgca gcccgcgtgg tcgggcgaac gacgcgcggc
     2821 gcgttgctcc agcccgtcgg ggcgcccgga atcgccgaac gccaggatcg gcagcctgcc
     2881 ccgggcgtcc tgcgccgcca gcaccatgtc ctcgagcaga tcgacctcgc acagcgccag
     2941 cgccggctgc gccttgtcga tcaccttgcc cagctcgagc gcgcgcaaca gcggcatggt
     3001 gggcaccacg atcagcccgg ccttgaccac cgccagccag gccagcgcca tgccgatcga
     3061 gttgccgccg cgcagcagca cgcggttgcc gggctcgagc ttgaagtccc ggatcagcat
     3121 ctcggcgatg cgagcgacct gcgcctcggt ctcggcgtag gtcagcgtct ggccgggtga
     3181 gcgcagcagc aggcggttgc tccagccgtt ggcgcgggcg gcgtcgaaca ggacggtgac
     3241 caggttggcc tgatccggaa tgcgcagctc cggcaggtcg tagcggaact cgggccactg
     3301 ctcgcgcggt ggcaggcgat cgtgcacgta acggtcggtc tgggcggatg ggacgctcat
     3361 ggtcatgcct cctgcagcac actcttgccg atgatgagtt gctggacttc ggtggcgccc
     3421 tcgtaaatgc gcagggcgcg aatgtcgcgg tacagcgatt cgaccttgcg gcccaccatc
     3481 acgcccatgc cgccgtgcat ctgcagcgcc atgtcgatca cgcgctgggc gttttcggtg
     3541 gcggtcatct tggccatggc ggcggccacg ctgcgctcct tggggctgcc ttgcccggtg
     3601 tcgcgcagcc aggcggcgcg gtaggtcagc agcgctgccc cgtcgatcag cgccgccatc
     3661 tcgcccagct tggcctgcgt gagctggaag tcggccaggg tctggccaaa catgcggcgc
     3721 tggcgcgcat gagcgatggc ctcggccagc gcgcgccgcg ccatgcccag ggccgcgccg
     3781 gcgacgctgg cgcggaaaat gtccagcgtc tgcatggcca gcttgaagcc gccattcaac
     3841 tcgcccagca tctgctcgcc cgccacgcgg cagcgctcga acaccagggt ggccagcggg
     3901 tgcggcgcca tcaccgg
//
LOCUS       DAOOOG010000210         3901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_28116_length_3901_cov_24.075923, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000210 DAOOOG010000000
VERSION     DAOOOG010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28116_length_3901_cov_24.075923"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1136
                     /locus_tag="PK242_12070"
     CDS             144..1136
                     /locus_tag="PK242_12070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ94796.1"
                     /translation="MQHTHSSRRQFLGLMGAGLAGSALVLPGAASAADTLGSRPVRII
                     VPFPAGGGGDTLTRMVGDALSQQLKLPVVVENKPGGDGVIAVQELLRAAPDGHTIMFG
                     TPSALLYTPQLTPKRPPYDPLKDLAPITHFSSFTYYVYVSDALPVKTMGELVDYVRKH
                     PGKVAYGTGDSTQLVAMAQLCDQAKLDMIHVHYKGTSQAFTDFMGGRIQVMVGGFDLE
                     EQSKGKALPLATLLPERSPLKPNVPTLGEAGLAPVTTRAWTAFFAPAKTPVPIIDQLN
                     KAFAATFQNPKLQDFFKSRGSVLQASTPDAMHELLVEQLPVFAKAIKTLNLYRD"
     gene            1174..2736
                     /locus_tag="PK242_12075"
     CDS             1174..2736
                     /locus_tag="PK242_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ94797.1"
                     /translation="MHEPTPIHHAAQAARRWPDKLALICTPSGQRCSYAELDAAANQA
                     AHALGQLGLAPGDGIAFCLPNGVAFAVLMLAALRTGLYYTPLPTKASAADIAYICNDA
                     GARLLVLDAASGAHAELPALLAPECPLIISQADPTQPGAWEKLLAQQPATTPTDPRPG
                     IEMVYSSGSTGKPKGVRKALPVRNWTDADPRNAAIASAAHLGDDSVYLSTSPLYHSAP
                     HRYLSASLNAGATVVVMERFDAQASLECIDRYDCTHSLWVPTMFHRLLKLDPSVRQRY
                     RGERHRVAIHGAAPCPVPLKRAMIDWWGPILVEYYSGSEGLGSTTIDSREWLAHPGSV
                     GKPRQVAVHVLDEQFQPLPPGEVGTIFFDAPSAFSYWRDDEKTRGATSPQGWKTYGDI
                     GYLDEEGYLYLTDRRSFTVISGGVNIYPQEIENTLLAHPDVEDVAVFGVPDEDLGEKL
                     AAVIQLSAGVTANAATALAMQAHCRAAGGSIKTPKLIRFCPEFPRTDSGKVQKARLRA
                     QFLQEHDAPTFG"
     gene            complement(2759..>3901)
                     /locus_tag="PK242_12080"
     CDS             complement(2759..>3901)
                     /locus_tag="PK242_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOZ94798.1"
                     /translation="LLENPRDDTSFLRVVNFPPRGIGARTLEQLQDAARAAGSSLHDA
                     IASVPGKAGANLGAFVARIDVLREQTAGHTLREIIEQMLDASGLIEHYRGEKEGQDRI
                     ENLDELVNAAESFVTQEGFGRDAVALPMDEQGQPLTQSPASQGLDPDAPRLNEPLQAD
                     ADTGETQSPLAAFLTHAALEAGDNQAQAGQDAVQLMTVHASKGLEFDAVFITGLEEGL
                     FPHENALSDQGGLEEERRLMYVAITRARKRLYLSHAQTRLLHGQTRYNLRSRFLDELP
                     EAVLKWLTPQRGGFAEVTSKQIAARADWTSARGRFGLNSSDTSPRPSASGQSICAGQA
                     VFHNKFGEGRVLAVEGSGDDARAQVNFPRHGTKWLALAVAKLTVVE"
BASE COUNT          650 a         1362 c         1229 g          660 t
ORIGIN      
        1 ggcgagctgg tgcagcgctt cattgatcag taccacgccg cacgccagcg catagacctg
       61 cgcacagcct gagcagcacc acgacgctga tatccccgca cacgcttcgc cttcaccctc
      121 ttgatcgcca cggagacata cacatgcaac acacccattc ttcgcgccgc cagtttctgg
      181 gcctgatggg cgccggcctg gctggcagtg cgctcgtcct gcccggcgcg gcctctgcgg
      241 ccgacacgct gggcagtcga ccggtgcgca tcatcgtgcc ctttccagct ggcggcggcg
      301 gcgacaccct gacgcgcatg gtgggcgacg cgctgtccca gcaactcaag ctgccggtgg
      361 tggtggagaa caagccgggt ggcgatggcg tgatcgccgt gcaggagctg ctgcgcgccg
      421 cacccgacgg ccacacgatc atgtttggca cgcccagtgc cctgctctac acaccgcagc
      481 tgacgcccaa gaggcccccg tatgacccgc tgaaggatct ggcaccaatc acccacttca
      541 gctccttcac ctactacgtt tatgtcagcg acgccttgcc ggtcaaaacc atgggcgaac
      601 tggtggacta cgtgcgcaag cacccgggca aggtggcgta tggcaccggc gattcgaccc
      661 agctggtggc catggcccaa ctgtgcgacc aagccaagct ggacatgatt catgtgcact
      721 acaagggcac ttcgcaggcg ttcaccgact tcatgggcgg gcgcatccag gtgatggtcg
      781 gcggctttga tctggaagaa cagtccaagg gcaaggcgct tccgctggcc acgctgctgc
      841 cagagcgcag cccgctcaag cccaatgtgc caacgctcgg tgaggcgggt ctggccccgg
      901 tgaccacccg ggcctggacg gcattctttg cccccgcgaa aacacccgtg ccgatcatcg
      961 atcagttgaa caaggcgttt gcggcgacgt tccagaaccc caagctgcag gacttcttca
     1021 aatcgcgcgg ctccgtgctg caggccagca cgcccgacgc catgcatgag ctcctggtcg
     1081 agcagttgcc cgtgttcgcc aaggccatca agaccctgaa cctgtatcga gactgattct
     1141 gcggcgcacg acggcgcctc ggacgcattc tgcatgcacg aacccacgcc catacaccac
     1201 gctgcgcagg ccgcgcgccg ctggcccgac aagctggcgc tgatctgcac cccgagcgga
     1261 cagcgctgca gctacgccga gctggacgcc gccgccaacc aggccgctca cgcactgggc
     1321 cagctgggcc tggcgcccgg tgatggcatt gccttttgcc tgcccaacgg cgtggccttt
     1381 gccgtcctca tgctggcagc gctgcgcacg ggtctctact acacacccct acccaccaag
     1441 gcgtcggcgg ccgacatcgc ctacatctgc aacgatgccg gcgcacggtt gctggtgctg
     1501 gacgcagcca gcggcgccca cgccgagctg cccgccctgt tggcgccaga gtgccccctg
     1561 atcatcagcc aggccgaccc cacgcaaccc ggcgcgtggg agaaactgct ggcgcaacaa
     1621 cccgcaacca ctccgacaga cccccgtccc ggcatcgaga tggtgtattc gtcgggctcg
     1681 accggcaaac ccaagggcgt gcgcaaggcg ctgcccgtgc gtaactggac cgacgcagac
     1741 ccccgaaacg ccgccattgc cagcgccgcg cacctggggg acgacagcgt ctacctatca
     1801 acgtcaccgc tctatcacag cgcaccgcac cgctatctgt ccgccagcct gaacgccggc
     1861 gcgacggtgg tcgtgatgga gcgctttgac gcccaagcca gcctggaatg catcgatcgc
     1921 tacgactgca cgcattcgct gtgggtgccc accatgttcc accggctgct caagctcgac
     1981 ccgtctgtac gtcaacgcta tcggggcgag cgccaccggg tggccattca cggcgcggcg
     2041 ccgtgcccgg tgccgctcaa gcgcgccatg atcgactggt ggggacccat tctggtcgag
     2101 tactactcgg gctccgaagg actgggcagc accaccatcg actcgcgcga gtggctggct
     2161 catcccggct cagtgggcaa gccgcgccag gtggcggtgc acgtactgga cgagcagttc
     2221 cagccgctgc ctccgggcga ggtgggcacc atcttcttcg atgcgccaag cgccttttca
     2281 tactggcgcg acgacgagaa gacccgtggt gccaccagcc cccagggctg gaagacctac
     2341 ggcgacatcg gttacctgga tgaggaaggc tacctgtacc tgaccgaccg gcgcagcttc
     2401 accgtgatct cgggtggcgt gaacatttat ccgcaggaga tcgaaaacac cctgctggcc
     2461 caccccgacg tcgaggacgt ggcggtgttc ggcgtgcctg acgaggatct gggtgaaaag
     2521 ctcgctgccg tcatccagct gagcgcgggc gtcaccgcca acgctgccac cgccctggcg
     2581 atgcaagccc attgtcgggc tgctggcggc agcatcaaga cacccaagct gattcggttt
     2641 tgccccgagt ttccgcgcac cgacagcggc aaggtgcaga aggcgcgtct gcgtgcgcag
     2701 ttccttcagg agcatgacgc gccgacgttt ggctgagacg gccgcaagcg ccgtcgccct
     2761 attccaccac cgtcagcttg gccaccgcca gcgccagcca cttggtgccg tggcgcggaa
     2821 aattgacctg ggcgcgtgca tcgtcaccac tgccttccac ggccagtacg cgaccttcac
     2881 cgaacttgtt gtgaaacacc gcctggccgg cgcaaatgct ctggccgctg gcggatgggc
     2941 gaggcgaggt gtccgaggaa ttcaagccaa atcggcctct agcgcttgtc cagtcagcgc
     3001 gagcagctat ttgttttgaa gtaacttcgg caaagccccc acgctgcggc gtgagccact
     3061 tgagcaccgc ttcgggcagc tcgtcgagaa agcggctgcg caggttgtag cgcgtctggc
     3121 cgtgcagcag gcgcgtctgc gcatggctca ggtacaggcg ctttctcgcg cgcgtgatgg
     3181 ccacgtacat caggcggcgc tcttcctcca ggccgccctg gtcgctcaag gcgttctcgt
     3241 gcggaaacag cccctcctcc agaccggtga tgaacacggc gtcgaactcc agccccttgc
     3301 tggcgtgcac ggtcatcagt tgcaccgcgt cctgcccggc ctgggcctgg ttgtcgcccg
     3361 cctccagggc ggcgtgggtg aggaaagcag ccaacgggct ctgcgtctcg ccggtgtcgg
     3421 cgtcggcttg cagcggctcg ttcagccttg gcgcgtcggg gtcgagcccc tggctggccg
     3481 ggctttgtgt gagcggctgg ccctgctcgt ccatgggcag cgccaccgca tcgcggccaa
     3541 agccctcctg cgtaacgaag ctttcggcgg cgttgaccag ttcgtcgagg ttctcgatgc
     3601 ggtcctgccc ttccttctcg ccgcggtagt gctcgatcag gccgctggcg tccagcatct
     3661 gctcgatgat ctcgcgcagg gtgtggccgg cggtctgctc gcgcagcacg tcgatacgcg
     3721 cgacgaaggc gcccaggttg gcgccggcct tgccgggcac gctggcgatg gcgtcgtgca
     3781 gcgagctgcc ggcggcgcgt gcggcgtcct gcagttgctc cagcgtgcgc gcgccgatgc
     3841 cgcgcggcgg gaagttgacc acgcgcagaa agctggtgtc gtcgcgcggg ttctccagca
     3901 g
//
LOCUS       DAOOOG010000211         3893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_28195_length_3893_cov_24.468473, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000211 DAOOOG010000000
VERSION     DAOOOG010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28195_length_3893_cov_24.468473"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PK242_12085"
     CDS             complement(<1..964)
                     /locus_tag="PK242_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOZ94799.1"
                     /translation="MPAAEVPTTAAANAAAASFAALEARLDYLDAADLERVRQAYRYA
                     DTAHLGQMRHSGEPYITHPIAVAAQVAEWKLDASALMAALLHDTLEDCGVSKADLLER
                     FGAQVAELVDGLTKLDKIQFSTREEGQAESFRKMLLAMARDVRVILVKLADRLHNMRT
                     MGDMPRSKWGRIATETLEIYAPIAHRLGLNQTYRELQDLSFRHLKPWRYSVLAKALAR
                     ARQRRGDLMQKLQHDLQAALADVGLNARIVGREKTLYSLYRKMDHKRQSFAQVNDLYG
                     VRIILPRVLDCYTGLGVLHQMFKPVPGRFKDYIAIPKVNGYQSLH"
     gene            complement(1092..1292)
                     /gene="rpoZ"
                     /locus_tag="PK242_12090"
     CDS             complement(1092..1292)
                     /gene="rpoZ"
                     /locus_tag="PK242_12090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOZ94800.1"
                     /translation="MARITAEDALEKIPNRFQLVLAATYRARMLNQGHAPKVESRNKA
                     GVTALREIAAGKIGLEMLKKVT"
     gene            complement(1317..1937)
                     /gene="gmk"
                     /locus_tag="PK242_12095"
     CDS             complement(1317..1937)
                     /gene="gmk"
                     /locus_tag="PK242_12095"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOZ94801.1"
                     /translation="MDHPGNLFVVAAPSGSGKSSLVKALMEVDAGVQPSISHTTRAPR
                     GQDKHGREYYFVDDAEFDAKIAAGDFLEWAHVHGRRYGTSRQAIEARIAAGGDVVLEI
                     DWQGALQIQRQFPDAILVFVLPPSWEELRARLQRRGEDTPEVIELRLANAREELAQVK
                     HFDYVIINEVFERALFDLKTIVHAQRLRYTAQKTARAETFQALGID"
     gene            complement(2000..2635)
                     /locus_tag="PK242_12100"
     CDS             complement(2000..2635)
                     /locus_tag="PK242_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HOZ94802.1"
                     /translation="MHSPTPDLPCSTAAERNRGPIWGVLRRLLAPSGHALEIASGTGQ
                     HAAHFAHALPGWSWQPSDVSDAGFATVQGWADRLQARNVRRPLRLDVLDPRWPSEGAP
                     FADGAFDLIFCANMLHISPWPTCAALMRGAARHLAPGGALVVYGPFIEADLPTAPSNQ
                     AFDAELRRRHPSWGLRDLEAVRTEAQGAGLALAERHALPANNLLLVFRARE"
     gene            complement(2766..3782)
                     /locus_tag="PK242_12105"
     CDS             complement(2766..3782)
                     /locus_tag="PK242_12105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOZ94803.1"
                     /translation="MNRKLSDIALSMLDLVPVREGRSVADALAVALQTAQHAERLGFT
                     RYWLAEHHNMAGIASSATAVLVGHIAGGTERIRVGSGGVMLPNHAPLVVAEAFGTLAE
                     LYPGRIDLGLGRAPGTDPLTMRALRRDRIETADDFPRDVAELQRLLDRPQPGQKLIAN
                     PGAGTRVPIWLLGSSLFSAQLAAERGLPYAFASHFAPGLLLQAIEVYRRGFRPSAVLD
                     KPYVMIGVPLVAAPTDAEAMFLATSIFARVLGILRGARGLLQPPQEGFLERLDAPARA
                     GIADFLACAVIGAPDTVRQGLQALADATDCDEMMFTCDIYEPALRLRALDIAMQVRQD
                     SAGA"
BASE COUNT          567 a         1253 c         1424 g          649 t
ORIGIN      
        1 tgtgcagcga ctggtagccg ttgaccttgg ggatggcgat gtagtccttg aagcgccccg
       61 gcaccggctt gaacatctgg tgcagtacgc ccaggccggt gtagcagtcc agcacgcgcg
      121 gcaggatgat gcgcacgcca tacaggtcgt tgacctgggc gaacgactgg cgcttgtggt
      181 ccatcttgcg gtagagggaa tacagcgtct tctcgcgccc gacgatgcgc gcgttcaggc
      241 ccacgtcggc cagcgcggcc tgcaggtcgt gctgcagctt ctgcatcagg tcgccgcggc
      301 gctggcgtgc gcgtgccagc gccttggcca gcacgctgta gcgccagggc ttgaggtggc
      361 gaaacgacag gtcctgcagc tcgcggtagg tctggttcag gcccaggcgg tgggcgatgg
      421 gggcgtagat ttccagcgtt tcggtggcga tgcggcccca cttgctgcgc ggcatgtcgc
      481 ccatggtgcg catgttgtgc aggcggtcgg ccagcttgac caggatgacg cgcacgtcgc
      541 gcgccatggc cagcagcatc ttgcggaagg actcggcctg accttcctcg cgggtgctga
      601 actggatctt gtccagcttg gtcagcccgt cgaccagctc ggccacctgg gcgccgaagc
      661 gctccagcag atcggccttg ctgacgccgc agtcttccag cgtgtcgtgc agcagcgctg
      721 ccatcagggc gctggcgtcc agcttccatt cggccacctg ggcggccacg gcgatcgggt
      781 gggtgatgta gggctcgccg ctgtggcgca tctgcccgag gtgggcggtg tcggcatagc
      841 ggtaggcttg acgcacgcgc tccagatcgg ccgcgtccag atagtccagt ctggcttcaa
      901 gcgcagcaaa gctggccgcg gccgcgttgg cggcggccgt ggtgggcact tcggcagctg
      961 gcatgaccga actcatgctc gtaatttaac cgccccgtgc gccatttgtg caactgcagc
     1021 aaaaaggcgc cccgcagggc gccctgagct gtggcgaacg ctggcacggc gggttgccgt
     1081 gcgcggtcgg ttcacgtcac tttcttgagc atttccaggc cgatcttacc ggcggcgatc
     1141 tcgcgcagcg ccgtcacgcc ggccttgttg cgcgattcca ccttgggggc atggccctgg
     1201 ttgagcatgc gggcgcggta ggtggccgcc agcacgagct ggaagcggtt ggggattttt
     1261 tcgagggcgt cttcggcggt gatgcgtgcc atggagcgtg agcggtgcgg gtggaatcag
     1321 tcgatgccga gggcttgaaa agtctcggcg cgggcggttt tctgggccgt gtagcgcagg
     1381 cgctgcgcgt gaacaatggt tttcaggtcg aaaagcgcgc gctcaaatac ctcgttgatt
     1441 ataacgtagt cgaagtgctt gacctgcgcc agctcctcgc gcgcattggc caggcgcagc
     1501 tcgatcacct ccggcgtgtc ctcgccgcgc cgctgcaacc gtgcacgcag ctcttcccag
     1561 ctgggcggca gcacgaacac caggatggcg tcgggaaact ggcgctgtat ctgcagcgcg
     1621 ccctgccagt cgatttccag caccacgtcg ccaccggcgg cgatgcgcgc ttcgatggcc
     1681 tggcgcgagg tgccgtaacg cctgccgtgg acatgggccc actcgaggaa gtcgccggcg
     1741 gcgatcttgg cgtcgaactc ggcgtcatcg acgaagtagt actcgcgccc gtgtttgtcc
     1801 tgcccgcgcg gggcgcgcgt ggtgtgcgat atggagggtt gcacgcccgc gtcgacctcc
     1861 atcagcgcct tgaccaagct cgatttgccc gagccgctgg gtgcggcaac gacgaataga
     1921 tttccagggt gatccatatg gggtcagcgc gggcagctat gcattcagga gttgggccgg
     1981 cacgctggtg gggcggattc tactcacggg cgcgaaacac cagcagcagg ttgttggctg
     2041 gcagggcgtg gcgttcggcc agcgccagac cggcgccctg cgcctctgtg cgcaccgcct
     2101 ccaggtcgcg caggccccaa ctggggtggc ggcggcgcag ctcggcgtcg aaggcctggt
     2161 tgctgggcgc ggtcggcaga tcggcttcga tgaaggggcc gtacaccacc agcgccccgc
     2221 cgggggccag gtggcgcgcg gcgccgcgca tcagcgccgc gcaggtgggc caggggctga
     2281 tgtgcagcat gttggcgcag aagatcaggt cgaaggcacc gtcggcaaac ggtgcgcctt
     2341 cgctgggcca tcgtgggtcg agcacgtcca gccgcagcgg gcgccgcacg ttgcgtgcct
     2401 gcagccgatc ggcccagcct tgcacggtgg caaacccggc atcgctgacg tcgctgggct
     2461 gccagctcca gccgggcagg gcgtgggcaa agtgggcggc gtgctgaccg gtgccgctgg
     2521 cgatttcgag cgcgtggccg ctgggcgcca gcagcctgcg cagcacgccc cagatcggcc
     2581 cgcggttgcg ctcggcggcg gtgctgcagg gcagatcggg tgtcggtgag tgcatggtga
     2641 tttcgggctt cagcggttca tggaactgcg caggtagcta tcataaatgt agcgatttcg
     2701 gggcgcgcgc cgccggtctg cggtgcagcc ggcaggtcct gggtcaggcc ccgccgccgc
     2761 gccattcatg cgccggcact gtcctgccgc acctgcatgg cgatgtccag cgcgcgcagg
     2821 cgcagcgccg gttcgtagat gtcgcaggtg aacatcatct cgtcgcaatc ggtggcgtcg
     2881 gccagcgctt gcaggccctg gcgcacggtg tccggcgcgc cgatcacggc gcaggccagg
     2941 aagtcggcga tgccggcgcg cgccggcgcg tccaggcgct ccagaaagcc ttcctgcggc
     3001 ggctgcagca gcccgcgcgc gccgcgcaga atgcccagca cgcgggcaaa gatgctggtg
     3061 gccaggaaca tggcctcggc gtcggtcggc gccgccacca gcggcacgcc gatcatcacg
     3121 tagggtttgt cgagcaccgc cgacggccga aagccgcgcc gatacacctc gatcgcctgc
     3181 agcagcaggc ccggcgcgaa gtgcgaggca aaggcatagg gcaggccgcg ctcggccgcc
     3241 agctgagccg aaaacaggct ggagcccagc agccagatcg gcacccgggt gccggcgccg
     3301 gggttggcga tcagcttctg cccgggctgc gggcggtcca gcaggcgctg caactcggcc
     3361 acgtcgcgcg gaaagtcgtc ggccgtctcg atgcggtcgc gccgcagcgc gcgcatggtc
     3421 agcgggtcgg tgccgggggc gcgccccagg cccaggtcga tgcggccggg atacagctcg
     3481 gccagggtgc cgaacgcttc ggccaccacc agcggcgcgt ggttgggcag catcacaccg
     3541 ccggagccta cgcggatgcg ctcggtgccg ccggcaatgt gcccgaccag cacggcggtg
     3601 gccgaactgg cgatgcccgc catgttgtgg tgttcggcca gccagtagcg ggtgaagccc
     3661 aggcgctcgg cgtgctgcgc ggtctgcagc gccaccgcca gcgcgtcggc gacgctgcgg
     3721 ccttcgcgca ccggcaccag gtcgagcatg gacaaggcga tgtcggacag ctttcggttc
     3781 atgggtcgat tgtgtcgcgt cgcacgatcg caaccggcca tccatatcca gtggccttgc
     3841 cggtcgctgg agggctctct atcatcgcgc ggatggggtt cgcgaggaag ggg
//
LOCUS       DAOOOG010000212         3887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_28247_length_3887_cov_26.776879, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000212 DAOOOG010000000
VERSION     DAOOOG010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28247_length_3887_cov_26.776879"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PK242_12110"
     CDS             <1..900
                     /locus_tag="PK242_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ94804.1"
                     /translation="VQASLFSHNIGLPPVVLLASDAVRQEVVPPVLAGERISALAITE
                     PGGGSDVAQLRTTARREGDEYVVNGEKIFITSGMRADWITLAVRTGESKGASGISLLL
                     VPGDSAGLSRARLDKMGWLCSDTAHLRFDGVRVPARYLLGEEGQGFKAIMGNFNGERL
                     GLAAGAIGYAEACYDEALAWAQQRQSFGQPLVAHQVIRHKLVDMRQRICATAAWLEAV
                     AARADAGDASPDWVAEVCTLKNQATQTMQFCADAGVQILGGMGYMRGTVCERIYREVK
                     VVTIGGGTEEIMKELAARQWGIA"
     gene            929..1732
                     /locus_tag="PK242_12115"
     CDS             929..1732
                     /locus_tag="PK242_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ94805.1"
                     /translation="MNVQFDFSGRHAVVFGGTTGINLGIAQAFARAGARVTVASRKQA
                     NVDAAVATLGDGALGLVADVRDEASVASALAQAAATHGPIDVLVSGAAGNFLAEVNGM
                     SSNAFKVVVDIDLIGSFHVARQAYAHLRKPGASIIFITAPQAVVAMPHQAHVGAAKAG
                     VDQFARVLALEWGPAGVRVNAISPGPIQGTEGMQRLAPQIQDGDATLRAMVPLGRMGT
                     PQDIAHLAMFLGSDAASYVSGAVIACDGGAQGMLTPMISGAIAAQAPAR"
     gene            1792..3387
                     /locus_tag="PK242_12120"
     CDS             1792..3387
                     /locus_tag="PK242_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ94806.1"
                     /translation="MIDMPATDALRRIRRVALGDFIHRSALKFGERTAVVDGDTRLNY
                     RELDARSSRFAHHLIGTIGRGRQVGMLCANSADMVVAYNGIHKSGNVWVPVNIRLDVA
                     GIDYILRHAEVAAVVVDEAFHAAPGVADMLTRLGVPLIVTMAAQAGTAIAGAVTLAQA
                     EAGQSAELPAIDIDSNAPALIMYTSGTTGNPKGAVHSHASVGAALTGNMAGFAVTEQD
                     VFSGVLPLFHCAQHGLAATVHLAGGCIVLLRGFVPDEVRASILRERYTVFTGLPMMYA
                     ALLADPAFRVDSMRLAIYAMAPIPRPLIAQIAGRMTPNVMLATGQTEMYPGTMSFRPL
                     EHPQLDANYWGVSLPHNETAVMDDEGRLLGEGEPGEIVHRGANAMLGYLKDPEATAAA
                     QRFGWHHTGDLGQWGPGGQLMFLDRKKDMIKTGGENVASVKVEAVLLAHPGVAGVGVI
                     GLPHPRWAEAVCAFVVKKPGAEVDEAALQTHCRQHLGGFEVPKLIRFVEQLPATSTGK
                     VQKHLLRREFEQIAAQAWTDEAA"
     gene            complement(3583..>3887)
                     /locus_tag="PK242_12125"
     CDS             complement(3583..>3887)
                     /locus_tag="PK242_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ94807.1"
                     /translation="ASQPIRFWANKYKTKFNEDPGVFSVYGYQIMDNFIQGAQRAGAS
                     LTTDSFIKAMNSAVIAPDMFGSDTLKFSATHRLGTERSRLSQIQDGRWKVVSDYFK"
BASE COUNT          605 a         1301 c         1363 g          618 t
ORIGIN      
        1 gtgcaggcca gcctgttctc gcacaacatc ggcctgccgc cggtggtgct gctggccagc
       61 gacgccgtca ggcaggaggt ggtgccgccg gtgctggcgg gcgagcgcat ctcggcgctg
      121 gccatcaccg agcccggcgg tggctcggac gtggcgcagc tgcgcaccac ggcgcgacgc
      181 gagggcgacg aatacgtggt gaatggcgag aagatcttca tcaccagcgg catgcgcgcc
      241 gactggatca ctctcgccgt gaggacaggt gaatccaagg gcgcttccgg catcagcctg
      301 ctgctggtgc cgggtgacag cgcgggtctt tcgcgcgcgc gcctcgacaa gatgggctgg
      361 ctttgctccg acaccgctca tctgcgcttc gatggcgtgc gcgtgccggc gcgctacctg
      421 ctgggcgaag aagggcaggg cttcaaggcc atcatgggca acttcaacgg cgagcgcctt
      481 ggcctggccg cgggtgccat cggttacgcc gaggcctgct acgacgaggc gctggcctgg
      541 gcccagcagc gccagagctt cggccagccg ctggtggcgc atcaggtgat tcgccacaag
      601 ctcgtcgaca tgcgccagcg catctgcgcc accgccgcct ggctggaggc cgtggccgcg
      661 cgcgccgacg ccggcgatgc ctcgcccgac tgggtggccg aagtctgcac cctgaagaac
      721 caggccacgc agacgatgca gttctgcgcc gacgccggcg tgcagatcct gggcggcatg
      781 ggctacatgc gcggcacggt gtgcgagcgc atctaccgcg aggtcaaggt cgtcaccatc
      841 ggtggcggca ccgaagaaat catgaaggag ctggcggcgc gccagtgggg catcgcatag
      901 agcgtgcccg ccatccacgg ggtcgatcat gaacgttcaa ttcgatttca gcggccgcca
      961 tgccgtggtc tttggcggca ccaccggcat caacctgggc attgcgcagg cgttcgcacg
     1021 cgccggcgcc cgggtcacgg tggccagtcg caagcaggcc aatgtggacg cggcggtggc
     1081 caccctgggc gacggcgccc tggggctggt ggccgatgtg cgcgacgagg ccagcgtggc
     1141 cagcgcgctg gcgcaggccg ccgccacgca cgggccgatc gacgtgctgg tgtcgggcgc
     1201 ggccggcaac tttctggccg aggtcaacgg catgtcgtcc aacgccttca aggtggtggt
     1261 cgacatcgac ctgatcggca gctttcacgt tgcccggcag gcgtacgcac acctgcgcaa
     1321 gcccggcgcc agcatcatct tcatcactgc gccgcaggcg gtggtcgcca tgccgcatca
     1381 ggcgcatgtg ggggcggcca aggccggcgt ggaccagttc gcgcgcgtgc tggcgctgga
     1441 atggggcccc gccggcgtgc gcgtgaatgc gatttcgccc ggaccgatcc agggcaccga
     1501 aggcatgcag cggctggccc cgcagatcca ggacggcgac gccacgctgc gcgccatggt
     1561 gccactgggc cgcatgggca ccccgcagga catcgcccat ctggccatgt tcctggggtc
     1621 ggacgccgcc agctacgtgt ccggcgccgt gattgcctgc gacggcggcg cgcagggcat
     1681 gctcacgccc atgatcagcg gtgccatcgc ggcccaggcg ccggcgcgct gagtggcggt
     1741 gcggcgagtt gcttcttcat cgttttcaag ccacatccaa cggagacttt catgatcgac
     1801 atgcccgcca ccgatgccct gcgccgcatt cgccgcgttg cgctgggcga tttcattcat
     1861 cgcagcgcac tcaagttcgg cgagcgcacc gccgtggtcg atggcgacac gcggctcaac
     1921 taccgcgagc tggacgcgcg cagctcgcgc tttgcccatc acctgatcgg caccatcggc
     1981 cgcggccggc aggtgggcat gctgtgcgcc aactcggccg acatggtggt ggcctacaac
     2041 ggcattcaca agtcgggtaa cgtctgggtg ccggtgaaca tccggctcga cgtggcggga
     2101 atcgactaca tcctgcgcca cgccgaggtg gcggcggtgg tggtggacga ggccttccac
     2161 gccgcgccgg gggtggccga catgctgacc aggctgggcg tgccgctgat cgtgaccatg
     2221 gccgcgcagg ccggcacggc gatcgccggc gccgtcacgc tggcgcaggc cgaagcggga
     2281 cagagcgccg agctgcccgc catcgatatc gacagcaacg cccccgcgct catcatgtac
     2341 accagcggca ccacgggcaa ccccaagggc gcagtgcact cgcacgcctc ggtgggcgcc
     2401 gcgctgacgg gcaatatggc gggctttgcc gtgaccgagc aggacgtgtt ctcgggtgtg
     2461 ctgccgctgt ttcactgcgc ccagcacggc ctggcggcca cggtgcatct ggccggcggc
     2521 tgcatcgtgc tgctgcgcgg cttcgtgccc gatgaggtgc gtgcctccat actgcgcgag
     2581 cgctacaccg tgttcacggg tctgccgatg atgtacgccg cgctgctggc cgacccggcg
     2641 ttccgcgtcg attcgatgcg cctggccatc tacgccatgg cgcccatccc caggccgctg
     2701 atcgcgcaaa tcgccgggcg catgacgccc aacgtgatgc tggccaccgg ccagaccgag
     2761 atgtaccccg gcaccatgag ctttcgcccg ctggagcatc cgcagctgga cgccaactac
     2821 tggggcgtgt cgctgccgca caacgaaacc gcggtgatgg acgacgaggg ccgcctgctg
     2881 ggcgagggcg agccgggcga gatcgtgcat cgcggcgcca acgccatgct gggctatctg
     2941 aaggaccccg aggccaccgc cgccgcgcag cgctttggct ggcatcacac gggcgatctg
     3001 ggccagtggg ggccgggcgg ccagctgatg ttcctcgacc gcaagaagga catgatcaag
     3061 accggcggcg aaaacgtggc cagcgtcaag gtcgaggccg tgctgctggc acatccgggt
     3121 gtggccggcg tgggcgtgat cggcctgccg cacccgcgct gggccgaggc ggtgtgcgcc
     3181 ttcgtcgtca agaaacccgg tgccgaggtg gatgaagccg cgttgcagac gcactgccgc
     3241 cagcacctgg gcggcttcga ggtgcccaag ttgatccgct tcgtcgagca gctgcccgcc
     3301 acctccaccg gcaaggtgca aaagcacctg ctgcgccggg agttcgagca gatcgccgcg
     3361 caggcctgga ccgacgaagc ggcgtaactc tcaaaacgat agctgttggc gcttgctgga
     3421 caagcgttaa agcggttttc ttggcatcag gcggcaggcg tccctgctgg cgctcaccgc
     3481 ccccaagcag aaagccgccc ctggggcggc tttctgctat ggcgcgcagc gcgggctacc
     3541 ctgaacaggg cagttggccg cgcccacccg ccgcttcggg ggttacttga agtagtccga
     3601 caccactttc cagcgcccat cctgaatctg cgacaggcgc gagcgctcgg taccgagacg
     3661 gtgggtggcg ctgaacttca gggtgtcgct gccgaacatg tcgggggcaa tgacggcgct
     3721 gttcatcgcc ttgatgaagc tgtcggtggt caggctggca ccggcgcgtt gcgccccctg
     3781 aatgaagttg tccatgatct ggtagccgta gaccgagaac accccgggat cttcgttgaa
     3841 cttggtcttg tacttgttgg cccagaagcg gatcggctgc gaggctt
//
LOCUS       DAOOOG010000213         3878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_28362_length_3878_cov_28.605284, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000213 DAOOOG010000000
VERSION     DAOOOG010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28362_length_3878_cov_28.605284"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..758
                     /locus_tag="PK242_12130"
     CDS             147..758
                     /locus_tag="PK242_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ94808.1"
                     /translation="MAIERPRYLPAEERRAATVEAVIELAAQQNPSDITTTAIADHMG
                     LTQGALFRHFPTKDAIVEATMSWVSERLLASVDKAAERAPSPAAALQAMFMAHVDFVA
                     RHPGVPRMLFGELQRAGDTLPKRMVRTLIQKYSTRLHRQLEAGRLQGEFDHSLDVDAA
                     ATLFIGTIQGLVMQSLLAGSATRIRRDAPRAFAIYRRGIGSAS"
     gene            752..1906
                     /locus_tag="PK242_12135"
     CDS             752..1906
                     /locus_tag="PK242_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ94809.1"
                     /translation="MMKLPTLQRRTLALAAVVVPMLVLFVYVALRSGPLAPVAVTVAT
                     VESRPITPSLFGIGTVQARYTYRIGPTVPGRVKHLQVHVGEQVKAGQVLGEMDPVDLD
                     QRIKAQQAGIKSADAALRQAEVREGFAQTQASRYEQLLAVRGVSEETVAIKRQDLAVA
                     RAALAAAREDGARLRAELQAMHAQRGNLRLVTPVDGLVASRDADPGTTVVAGQAVVEL
                     IDPASLWVDTRFDQISAQGLAAGLPAQVVLRSRRNQALAARVLRIEPRADAVTEETLV
                     KVVFEQPPTPLPPVGELAEITVRLPELPAAPTIPNAALRTVGGKLGVWKLADADLHFA
                     PVEIGRTDLDGQAQVLKGLDAGDRVVVYSEKALGARTRTHVVERIAGVSP"
     gene            1903..3102
                     /locus_tag="PK242_12140"
     CDS             1903..3102
                     /locus_tag="PK242_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ94810.1"
                     /translation="MISLAGRDILQAWGKFIFTGVGLGLLIGVTLTMAGVYRGMVDDG
                     RVLLDSSSADLWVVQKDTLGPYAESSSIPDDLWRGIRAMPGVAQAANVTYLTMQVRKG
                     GEDVRAMVVGVAAGEPGTPGWPPQLVAGRQITRGHYEAVADLAAGFQLGDVLKIRRNH
                     YTVVGLTRRMVSSSGDPMVFVPLKDAQEAQFLKDNDAILMQRRRTEANPAFNRPGVPG
                     LLDAVIATQTSNSSVNAVLVRLRPGFEPEQVAEPIRRWKRLTVYDRAQMESILIGKLI
                     ATSARQIGMFLVILAAVSAAIVAFIIYTLTMDKIREIAVLKLIGTRNRTIAAMILQQA
                     LVLGVIGFVVGKISATFAAPIFPKYVLLVPADSIAGFAAVLAICVLASVVAIRVALKV
                     DPADAIG"
     gene            3107..3817
                     /locus_tag="PK242_12145"
     CDS             3107..3817
                     /locus_tag="PK242_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ94811.1"
                     /translation="MSTQGIRIENLSKRYGEGDTAVLALRNVNMEVAPGEVVGLIGPS
                     GSGKSTLLKCLGAVIDPTAGRMVLNDEVIFDDGWKVRDLRALRRDKIGFVFQAPYLIP
                     FLDATDNVALLPMLAGVPNGEARQRALELLTALDVQHRAQAMPSRLSGGEQQRVAIAR
                     GLVNRPPVILADEPTAPLDSERAMAVIRILNDMARRFQTAIIVVTHDEKIIPTFKRIY
                     HIRDGVTHEEAGEGRGFE"
BASE COUNT          614 a         1277 c         1372 g          615 t
ORIGIN      
        1 gtctgctggc gcgggttgat gagccattgc tggtcgtggc gcgcggatgt tctggtgttc
       61 ggacatcggt gtgctcgacc cttgcattga cagggatttg cgtgtcgata agatagtgac
      121 aaattacttt ctttgtgagt ttcgtcatgg ccattgagcg accgcgttac ctgccggccg
      181 aggagcgtcg ggcggctacc gtcgaggcag tgatagaact ggcggcgcag cagaacccca
      241 gcgacatcac gaccactgcg attgccgatc acatgggcct gacccaaggc gcgctgttca
      301 ggcactttcc gaccaaggat gcgatcgtgg aggcgaccat gtcgtgggtc agcgaacgtc
      361 tgttggccag cgtggacaag gcggccgagc gggcaccgtc accggctgca gcgctgcaag
      421 cgatgttcat ggcacacgtt gacttcgtgg ccaggcatcc gggtgtgccg cgcatgctgt
      481 tcggtgaatt gcagcgcgcg ggtgacacgt tgcccaagcg catggtaaga accctgatcc
      541 agaagtacag cacccgcctg caccgtcagt tggaagccgg ccggttgcaa ggcgagttcg
      601 atcacagtct tgacgttgac gccgctgcaa cgttgttcat cggcaccatc cagggcctgg
      661 tgatgcagtc cctgctggcg ggcagcgcca cccgcattcg ccgcgacgcg ccgcgtgcgt
      721 ttgcgatcta ccgtcgcggc atcgggagcg catcatgaaa ttgcccacgc ttcaacggcg
      781 cacgctggcg ctggcggccg tcgtcgtgcc gatgctcgtg ctgtttgtct acgtcgccct
      841 gcgctcggga ccgctggcgc cggtggcggt gacggtggcc acggtcgagt cgcggcccat
      901 caccccttcg ctgttcggca tcggcacggt gcaggcgcgt tacacctaca ggatcggtcc
      961 cacggtgccc ggacgggtca agcaccttca ggtccatgtg ggcgagcagg tcaaggccgg
     1021 ccaggtgctg ggcgagatgg atccggtcga tctggaccag cgcatcaagg cccagcaggc
     1081 gggcatcaag agcgccgacg ccgcgctgcg ccaggccgaa gtgcgcgaag gctttgccca
     1141 gacccaggcc agccggtacg agcaactgct ggccgtgcgt ggcgtcagcg aagagacggt
     1201 ggccatcaag cgccaggacc tggcggttgc ccgtgccgcg ctggcggccg ctcgcgaaga
     1261 cggggcccgg ttgcgcgccg aactgcaggc catgcacgcg caacgcggca acctgagact
     1321 ggtgacgccc gtggacggcc tggtggcatc gcgcgacgcc gatcccggca ccaccgtggt
     1381 ggccggacag gcggtggtgg aactgatcga tccggccagc ctgtgggtgg acacgcgctt
     1441 cgaccagatc agtgcccagg gcctggccgc cggcctgccg gcgcaggtgg tgctgcgctc
     1501 gcgccgcaac caggccctgg ccgcccgcgt gctgcgcatc gagccgcggg ccgatgcggt
     1561 gacggaggag acgctggtca aggtcgtgtt cgagcagccg cccacgccgc tgccaccggt
     1621 gggtgaactg gcggaaatca cggtgcgcct gcccgagctg ccggccgcgc ccaccatccc
     1681 gaacgcggcg ctgcgcaccg tgggcggcaa gctgggcgtg tggaaactcg cggatgcaga
     1741 ccttcacttt gcgccggtcg agatcgggcg caccgacctg gatggacaag cccaggtgct
     1801 caaggggctg gacgccggcg atcgggtggt ggtctacagc gagaaggcgc tcggcgcgcg
     1861 cacccgcacg cacgtggtcg agcgcattgc cggggtctcg ccatgatcag cctggccggg
     1921 cgcgacatcc tgcaggcctg gggcaagttc atcttcacgg gcgtggggct gggcctgctg
     1981 atcggcgtga ccctgaccat ggccggcgtc taccgcggca tggtcgatga tgggcgcgtg
     2041 ctgctggaca gcagcagcgc cgacctgtgg gtggtgcaga aggacacgct cgggccctac
     2101 gccgaatcgt ccagcattcc ggacgacctg tggcgcggca tccgcgccat gcccggcgtc
     2161 gcgcaggcgg ccaacgtgac ctacctgacc atgcaggtgc gcaagggcgg cgaggacgtg
     2221 cgcgccatgg tggtgggcgt ggcggccggc gagccgggca ccccgggctg gccgccccag
     2281 ctggtggccg ggcgccagat cacgcgcggg cattacgagg cggtggctga cctggccgcg
     2341 ggctttcagc tgggcgacgt gctgaagatc cggcgcaatc actacaccgt cgtcggcctg
     2401 acgcgccgca tggtgtcgtc ctcgggcgat ccgatggttt tcgtcccgct caaggacgcc
     2461 caggaggcgc agtttctcaa ggacaacgac gccatcctga tgcagcgccg ccgcaccgag
     2521 gccaacccgg ccttcaaccg cccgggcgtg ccggggctgc tggacgccgt gatcgccacg
     2581 cagaccagca acagctcggt caatgcggtg ctggtgcgcc tgcggccggg cttcgagccc
     2641 gagcaggtgg ccgagccgat ccggcgctgg aagcgcctga cggtgtacga ccgcgcgcag
     2701 atggagagca tcctgatcgg caagttgatc gccacctcgg ccaggcagat cggcatgttc
     2761 ctggtcatcc tggcggcggt cagcgcggcg atcgtggcct tcatcatcta cacgctgacg
     2821 atggacaaga tccgcgagat cgccgtgctc aagctgatcg gcacgcgcaa ccgcaccatc
     2881 gccgccatga tcctgcagca ggcgctggtg ctgggcgtga tcggcttcgt ggtcggcaag
     2941 atcagcgcca cctttgccgc gccgatcttc cccaagtacg tgctgctggt gcccgcggac
     3001 tcgatcgccg gctttgccgc cgtgctggcg atctgcgtgc tggccagcgt ggtggcgatc
     3061 cgcgtggcgc tcaaggtcga tccggccgat gcgatcgggt gacgtgatga gcacgcaagg
     3121 catccgcatc gaaaacctga gcaagcgcta cggcgagggc gacaccgccg tgctggcgct
     3181 caggaacgtg aacatggagg tcgcgcccgg cgaggtggtg ggcctgatcg gcccctccgg
     3241 ctcgggcaag agcaccctgc tcaagtgcct gggcgcggta atcgacccga ccgccggccg
     3301 catggtgctg aacgacgagg tgatcttcga cgacggctgg aaggtgcgcg acctgcgcgc
     3361 gcttcggcgc gacaagatcg gcttcgtgtt ccaggcaccc tacctgatcc cgttcctgga
     3421 cgccaccgac aacgtggcgc tgctgcccat gctggccggc gtgcccaatg gcgaggcccg
     3481 ccagcgcgcg ctcgagttgc tcaccgcgct cgacgtgcag caccgggcgc aggccatgcc
     3541 ctcgcgcctg tccggtggcg agcagcagcg cgtggccatc gcgcgcgggc tggtcaaccg
     3601 cccgcccgtg atcctggccg acgagccgac ggcgccgctg gactcggagc gcgcgatggc
     3661 ggtgatccgc atcctcaacg acatggcgcg gcgcttccag accgcgatca tcgtcgtcac
     3721 gcacgacgag aagatcatcc cgaccttcaa gcgcatctac cacatccgcg acggcgtcac
     3781 gcacgaggaa gctggcgagg gaagggggtt cgaatgagcg ccgcggacga cctggcgcac
     3841 gggagcgacc gctcatgaaa ctgagctttc tgggtgcc
//
LOCUS       DAOOOG010000214         3859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_28526_length_3859_cov_27.192166, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000214 DAOOOG010000000
VERSION     DAOOOG010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28526_length_3859_cov_27.192166"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="ttcA"
                     /locus_tag="PK242_12150"
     CDS             <1..849
                     /gene="ttcA"
                     /locus_tag="PK242_12150"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HOZ94812.1"
                     /translation="EKRLARQVGQAIADFNMIEEGDRVMVCLSGGKDSYGLLDILLKL
                     QARAPVRFELVAVNLDQKQPGFPAEVLPGYLRQLGIEHHIETQDTYSIVKAKIPEGKT
                     TCSLCSRLRRGILYSVARRLRCNKIALGHHRDDMLQTFFLNMFFGGKLKGMPPKLTSD
                     NGEFIVIRPLAYVAESDLVAWAEHRQFPIIPCNLCGSQENLQRKQVGEMLRQWQRQHP
                     GRIETMFTALQNVVPSHLMDGALHDFKHLRATGVADENGDKAFDEEVFAPPAGVPFAS
                     VTRVSA"
     gene            882..1532
                     /locus_tag="PK242_12155"
     CDS             882..1532
                     /locus_tag="PK242_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94813.1"
                     /translation="MWIARVALVLIAILLSACASGPRVVDALVRTHAAQPPGAAVLQG
                     AHYRFEHAPARVGQPAPERLQALAQAALARVGAVRDDAGARVSVQVDGTISAYWRDPW
                     YEPSNPRVTLGLGVGHAWRGGAIGLGWGWPMGGDSVPAYASEVSLLMRDLQSGQIVYD
                     TRARHDGPWHDTDAVLAALFVAALEGYPQPAASERRVAVPLLPPAAAASAATPAQR"
     gene            complement(1537..2763)
                     /locus_tag="PK242_12160"
     CDS             complement(1537..2763)
                     /locus_tag="PK242_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ94814.1"
                     /translation="MTAPTRGASGAWTALLLLATGFLMSQAFRTIAAIMAAPLQAELQ
                     LTPQQLGTFAAAYHFAFGGLQLFMGMGIDLHGLRRTVLTASPLMALGAAVSALATGFP
                     MLVAGQVLIGIGSAPAFLVCTIYVARHFAPERYTVVSSLILAVGGIGMLLTGSPLAWL
                     IEATSWRMGFWVLAGCALLSWLAIALGVHEPAPAAGARRESLGQALRTFGALCRLPHT
                     AGIVALALVSYGAFITLRGLWLGPLLMQRHGWSLVAAGNLAVLLTVVSLISLPLFGRA
                     DPGPRRRRRWMLWGSAVAALCFVGLAANGGMWLDVVLMLAFMMLSGYGTLQYPDVKDA
                     YEPAVIGRAMALFTMALFMGVALMQWATGAIAQAAQARGLDPFGAVFGAIAVLLALGA
                     LAFRLLPQPASVRGDQ"
     gene            2894..3544
                     /locus_tag="PK242_12165"
     CDS             2894..3544
                     /locus_tag="PK242_12165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOZ94815.1"
                     /translation="MQATRILAVRHGETAWNVDGRIQGHLDIALSDVGRAQAHRLGRA
                     LAEREPLALIVSSDLARARETARIVADATGAPLRTTTALRERCFGDLQGQRFAEIEAS
                     WPEQAERWRRREPDWAPPGAGGESLLQFRERVAQAVQALAAENMGNHIALFTHGGVLD
                     VLYRLATGLDLQAARSWQIGNAAVNRLLWTPDSGLSLVGWADTTHLEDESLDERFS"
     gene            3528..>3859
                     /locus_tag="PK242_12170"
     CDS             3528..>3859
                     /locus_tag="PK242_12170"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOZ94816.1"
                     /translation="MNASADLIGPRLRALIGQGVDAIDTPALVVDLDAANRNLQRMSA
                     FASRYGLRLRPHAKMHKSAQFAHLQLQAGAVGLCVQTVGEAEALAAGGVNDIFITNEL
                     LAVGKLRRV"
BASE COUNT          615 a         1414 c         1358 g          472 t
ORIGIN      
        1 gaaaagcgcc tggcccgcca ggtcggccag gcgatcgccg acttcaacat gatcgaggaa
       61 ggcgaccgcg tgatggtgtg cctctcgggc ggcaaggaca gctacggcct gctggacatc
      121 ctgctgaagt tgcaggcgcg cgcgccggtg cgcttcgagc tggtggcggt caacctggac
      181 cagaagcagc ccggctttcc ggccgaggtg ctgccgggct acctgcggca gctcggcatc
      241 gagcaccaca tcgagacgca ggacacctac agcatcgtca aggccaagat tcccgagggc
      301 aagaccacgt gcagcctgtg ctcgcgcctg cgccgcggca ttctgtacag cgtggcacgg
      361 cgcctgaggt gcaacaagat cgcgctgggc caccaccgcg acgacatgct gcaaaccttc
      421 tttctcaaca tgttcttcgg cggcaagctg aagggcatgc cgcccaagct gaccagcgac
      481 aacggcgagt tcatcgtcat ccgcccgctg gcctacgtgg cggaaagcga cctggtggcc
      541 tgggccgagc accgccagtt ccccatcatc ccctgcaacc tgtgcggcag ccaggagaat
      601 ctgcagcgca agcaggtggg cgagatgctg cggcagtggc agcgtcagca cccggggcgc
      661 atcgaaacca tgttcaccgc gctgcagaac gtcgtgccct cgcacctgat ggatggcgcg
      721 ctgcacgact tcaagcacct gcgggccacc ggcgtggccg acgagaacgg cgacaaggcc
      781 ttcgacgagg aggtgttcgc cccaccggca ggcgtgccgt ttgccagcgt gacgcgggtc
      841 agtgcctgag gtttcacatc catatcaagg gggtagcgcc catgtggatt gcgcgagtag
      901 ctcttgtttt aatagcgatt ttgctgagcg cctgcgccag cggcccgcgc gtggtggacg
      961 ccctggtgcg cacccacgcc gcccagccgc cgggcgccgc cgtgctgcag ggcgcgcatt
     1021 accgcttcga gcacgccccg gcgcgcgtgg gccagccggc gcccgaacgg ctgcaggccc
     1081 tggcccaggc ggcgctggcg cgcgtgggcg cggtgcgcga cgacgccggc gcgcgcgtca
     1141 gcgtgcaggt ggatggcacg atcagcgcct actggcgcga cccctggtac gagcccagca
     1201 acccgcgcgt cacgctgggc ctgggcgtgg gccatgcctg gcgcggcggc gccatcggcc
     1261 tgggctgggg ctggccgatg ggcggcgaca gcgtgccggc ctacgccagc gaggtcagcc
     1321 tgctgatgcg cgatctgcag agcggccaga tcgtctatga cacgcgggcc cgccacgacg
     1381 gcccctggca cgacaccgac gccgtgctgg cggcactgtt cgtggccgcg ctggagggct
     1441 acccgcagcc cgccgccagc gagcgccgcg tggccgtgcc gctgctgccg ccggccgccg
     1501 ccgcgtctgc cgcgacgccg gcgcagcgtt gacccctcat tgatcgccgc gcacgctggc
     1561 cggctgcggc agcaggcgaa aggccagcgc gcccagcgcc agcagcacgg cgatggcgcc
     1621 gaacaccgcg ccgaacgggt ccaggccgcg cgcctgcgcc gcctgcgcga tggcgccggt
     1681 ggcccactgc atcagcgcca cgcccatgaa cagcgccatg gtgaacagcg ccatggcgcg
     1741 gccgatcacc gccggctcgt aggcgtcctt gacgtccggg tactgcaagg tgccgtagcc
     1801 cgacagcatc atgaacgcca gcatcagcac cacgtccagc cacatcccgc cgttggcggc
     1861 caggccgacg aagcacagcg cggccacggc gctgccccac agcatccagc ggcggcgccg
     1921 gcgcggcccc gggtcggcgc ggccgaacag cggcaggctg atgagcgaga ccaccgtcag
     1981 cagcaccgcc aggttgccgg ccgccaccag gctccagccg tggcgctgca tcagcagcgg
     2041 gcccagccac agcccgcgca gggtgatgaa ggcgccgtag ctcaccagcg ccagcgccac
     2101 gatgcccgcg gtgtgcggca gccggcacag cgcgccgaag gtgcgcagcg cctggcccag
     2161 gctctcgcgc cgcgcgccgg cggccggcgc cggttcgtgc acgcccagcg cgatcgccag
     2221 ccacgacagc agcgcgcagc cggccagcac ccagaagccc atgcgccagg acgtggcctc
     2281 gatcagccag gccagcgggc tgccggtcag cagcatgccg atgccgccca ccgccaggat
     2341 caggctggac accaccgtgt agcgctcggg cgcgaaatgc cgcgccacgt agatggtgca
     2401 gaccaggaat gccggtgcgc tgccgatgcc gatcagcacc tggcccgcca ccagcatcgg
     2461 aaagccggtg gccagcgccg acaccgccgc gcccagggcc atcagcggcg aggccgtcag
     2521 caccgtgcgg cgcagcccgt gcaggtcgat gcccatgccc atgaacagct gcaggccccc
     2581 gaaggcgaag tggtaggccg ccgcaaaggt gcccagctgc tgcggcgtca gttgcagctc
     2641 ggcctgcagc ggcgccgcca tgatggcggc gatggtgcga aacgcctggc tcatcagaaa
     2701 gccggtggcc agcagcagca gggcggtcca ggcgccggag gcgccgcggg tgggtgcggt
     2761 catgggcaag caagcggagg ccgcacagcg gcgcaaccgg caagcctaaa cgcttttgcg
     2821 cgggcgcacc ggtcgcctgc gggacgcggt cgcgtggcct gccgcccacc ggccgcgtgg
     2881 catgatccgg cgcatgcaag ccacccgcat cctggccgtc cgccatggcg aaaccgcctg
     2941 gaatgtggac ggccgcatcc agggccacct ggacatcgcg ctgagcgacg tcggccgcgc
     3001 ccaggcgcac cggctggggc gtgcgctggc cgagcgcgag ccgctggcgc tgatcgtcag
     3061 cagcgacctg gcgcgcgcgc gggagaccgc gcgcatcgtc gccgacgcca ccggcgcgcc
     3121 gctgcgcacc accacggcac tgcgcgagcg ctgcttcggc gacttgcagg gccagcgctt
     3181 cgccgagatc gaagccagct ggcccgagca ggccgagcgc tggcgccgac gcgagccgga
     3241 ctgggcgcca ccgggcgccg gcggcgaatc gttgctgcaa tttcgagagc gcgtggcgca
     3301 ggcagtgcaa gcgctggcgg ctgaaaacat gggcaatcac atcgccctgt tcacgcatgg
     3361 cggcgtgctc gacgtgctgt accgcctggc caccgggctg gacctgcagg ccgcgcgcag
     3421 ctggcagatc ggcaacgccg ccgtcaaccg cctgctgtgg acgcccgaca gtggcctgtc
     3481 gctggtcggc tgggccgaca ccacccacct ggaagacgaa agcctggatg aacgcttcag
     3541 ctgaccttat cggaccgcgc ctgcgtgccc tgatcggcca gggcgtggac gccatcgaca
     3601 cgcccgcgct ggtggtcgac ctggacgccg ccaaccgcaa cctgcagcgc atgagcgcct
     3661 tcgccagccg ctacggcctg cgcctgcgcc cgcacgccaa gatgcacaag agcgcccagt
     3721 tcgcccacct gcagctgcag gccggcgccg tcggcctgtg cgtgcagacc gtgggcgagg
     3781 ccgaggcgct ggccgccggc ggcgtcaacg acatcttcat caccaacgag ctgctggccg
     3841 tcggcaagct gcgccgcgt
//
LOCUS       DAOOOG010000215         3846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_28652_length_3846_cov_29.526510, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000215 DAOOOG010000000
VERSION     DAOOOG010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28652_length_3846_cov_29.526510"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="PK242_12175"
     CDS             <1..1592
                     /locus_tag="PK242_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOZ94817.1"
                     /translation="ITMFAWLAAALVGALWRRSARWGWPAGRSPCLWLPAPTAARLGG
                     LLLATAYALFSGWGVPAQRTVLMLAALTLLHVAGLRWPWWLNALAAAVVVLALDPWAL
                     MQPGFWLSFVAVAVLFATDSRAAGAQQTGARARWLALLREQGVVTLALTPLSLILFGQ
                     ASVVGLLANLLAIPWVTLLVTPLALLGVLWAPLWALAGAALWPLAALLGWLAAWPWAS
                     VSLPAAPWWLGVAAVAGGLLLALRWPPAWRLAGLPLVLPLALWQPARPAPGQFELLAA
                     DVGQGSAVLVRTARHSLLYDAGPRYSLESDAGHRVLVPLLRALGERLDSVVLSHRDSD
                     HTGGARAVLAAQPQAELRASIEDGHELQALRPATRCQAGQRWQWDGVAFELLHPDAAD
                     HARPLKSNALSCVLRVRAVDGAAALLTGDIEQAQEQALVARTPELRADVLLAPHHGSR
                     GSSSAALLAAVRPRWVLVQAGYRNRFGHPAAEALARYAAVGAQPVGSPRCGAMRWHSA
                     QPGALRCERDTAARYWHHQPP"
     gene            1589..2080
                     /locus_tag="PK242_12180"
     CDS             1589..2080
                     /locus_tag="PK242_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOZ94818.1"
                     /translation="MKTLLIVYHSLTGGTRQMAEAAAQAARQEGGVAVRLLTAAQAGP
                     PDLLAADGYLFATPENLAAMAGLMKDFFDRCYYPVLERIQGRPYAAMICAGSDGAGAL
                     RQLERIATGWRLKQVAPGLIVCTHAQTPERILAPKTIEPPDLARCAELGAGLAAGLGA
                     GVF"
     gene            complement(2102..3187)
                     /gene="mltB"
                     /locus_tag="PK242_12185"
     CDS             complement(2102..3187)
                     /gene="mltB"
                     /locus_tag="PK242_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HOZ94819.1"
                     /translation="MTAPRLPFDRRRLLATICIAAGALPVSAAAQNGPKRRRLPEAAP
                     SSYASRPDALRWAEDMAARRDLPPDWVRDTIGQAQHLPNVPRLMLPPARGTAKNWRAY
                     RSRFIDATRIRAGARFWQRNRATLARAQQDYGVPPEIVVGIIGVETIYGQNMGSFRVL
                     DALATLSFDFPPQHPRAAARQAFFQDELAQYLSLCQRSGMDPQQPLGSYAGAMGMPQF
                     MPSSWVRYAIDFDADGHIDLWGSEGDAIGSVAHYFQGYGWQPGQPTHYPVQLAPEADL
                     DRLLAPDILPSFRPARFAELGARLDAAGQAHDGPLALIELQNGAAEPSYVAGTENFYV
                     ITRYNWSSYYAMAVIELGQEVAAALGA"
     gene            complement(3162..>3846)
                     /locus_tag="PK242_12190"
     CDS             complement(3162..>3846)
                     /locus_tag="PK242_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOZ94820.1"
                     /translation="DGYWVVRNADAHAWTEVWDPGRGWLRVDPTAAVQPARIGQLQRL
                     RRPEGLVAGALGTLSPTLMAQLRSLWEAANNGWNQWVLNYTQSRQLDLLRQLGFASPN
                     WTDLGKVLAGLLVAAALLGMAWARWERSQHDPWLRLLARARRLLAQAGLNAPPQAPPR
                     ALAQQVAHSTLPAALRQPLTDWLLALETLRYAPSQAAAPRLTTLQRELRRLPWPRHPT
                     RHDRTPPAL"
BASE COUNT          427 a         1350 c         1531 g          538 t
ORIGIN      
        1 acatcaccat gttcgcctgg ctggcggcgg cgctggtggg tgcgttgtgg cggcgttcgg
       61 cgcgctgggg ctggccggcc ggccgcagcc cctgcctgtg gctgccggca ccgacggcgg
      121 cacggctggg cggcctgctg ctggcgacgg cctatgcgct gttcagcggc tggggcgtgc
      181 cggcgcagcg cacggtgctg atgctggccg cgctgacgct gctgcacgtc gccggcctgc
      241 gctggccctg gtggctgaat gcgctggcgg cggcggtggt ggtgctggcg ctggacccgt
      301 gggcgctgat gcagcccggc ttctggctca gcttcgtcgc cgtggcggtc ctgtttgcta
      361 ccgattcgag agcggccggc gcacaacaga cgggcgccag agcccgatgg cttgccttgc
      421 tgcgggagca gggcgtggtc acgctggcgc tcacgccgct gtcgctgatc ctgttcggtc
      481 aggcttcggt ggtgggcctg ctggccaatc tgctggccat tccctgggtc acgctgctgg
      541 tcacgccgct ggcgctgctg ggcgtgctgt gggcgccgct gtgggcactg gccggcgccg
      601 cgctgtggcc gctggcggcg ctgctgggct ggctggcggc ctggccctgg gccagcgtgt
      661 cgctgccggc ggcgccgtgg tggctgggcg tggcggcggt ggccggcggg ctgctgctgg
      721 cgctgcgctg gccgccggcg tggcggctgg ccggcctgcc gctggtgctg ccgctggcgc
      781 tgtggcagcc ggcgcggccg gcgccggggc agttcgaact gctggcggcc gacgtcgggc
      841 agggcagcgc ggtcctcgtg cgcaccgcgc gccacagcct gctgtacgac gccgggccgc
      901 gctactcgct cgaaagcgat gccggccatc gcgtgctggt gccgctgctg cgcgcgctgg
      961 gcgagcgcct ggacagcgtg gtgctgagcc accgcgacag cgaccacacc ggcggcgctc
     1021 gcgccgtgct ggcggcgcag ccgcaggccg agctgcgcgc gtcgatcgag gacgggcacg
     1081 agctgcaggc gctgcgcccg gccacccgct gccaggccgg ccagcgctgg cagtgggacg
     1141 gcgtggcctt cgagctgctg caccccgacg ccgccgacca cgcccgcccg ctcaagtcca
     1201 acgccctcag ctgcgtgctg cgcgtgcgtg cggtcgacgg cgccgccgcc ttgctgaccg
     1261 gcgacatcga gcaggcgcag gagcaggcgc tggtggcgcg cacgcccgag ctgcgcgccg
     1321 acgtgctgct ggcgccgcac cacggcagcc gcggttcgtc gtcggccgcg ctgctggcgg
     1381 cggtgcgccc gcgctgggtg ctggtgcagg ccggctaccg caaccgcttc ggtcacccgg
     1441 cagccgaggc gctggcgcgc tacgccgccg tcggtgcgca gccggtcggc tcgccgcgct
     1501 gcggcgccat gcgctggcac agcgcgcagc ccggcgcgct gcgctgcgaa cgcgacacgg
     1561 cggcacgcta ctggcatcat caaccgccat gaagacgctg ctgatcgtct accacagcct
     1621 gaccggcggc acgcgccaga tggccgaggc ggcggcccag gccgcgcgcc aggagggcgg
     1681 cgtggcggtg cgcctgctga ccgccgcgca agccggcccg ccggacctgc tggcagccga
     1741 cggctacctg ttcgccacgc cggagaacct ggcggccatg gccgggctga tgaaggactt
     1801 cttcgaccgc tgctactacc cggtgctcga acgcatccag gggcggccct acgccgccat
     1861 gatctgcgcc ggcagcgacg gcgccggcgc gctgcgccag ctcgaacgca tcgccaccgg
     1921 ctggcggctg aagcaagtgg cgcccggcct gatcgtctgc acccacgccc agacgcccga
     1981 gcgcatcctg gcgcccaaga ccatcgagcc gccggacctg gcgcgctgcg ccgagctggg
     2041 cgccgggctg gcggcggggc tgggggcggg ggtgttctga gcgcggccgc gccggccccc
     2101 gctacgcgcc cagcgccgcc gccacttcct gccccagctc gatcacggcc atggcgtagt
     2161 agctgctcca gttgtagcgg gtgatgacgt agaagttttc ggtgccggcc acgtagctgg
     2221 gttcggcggc gccgttctgc agctcgatca gcgccagcgg gccgtcgtgg gcctggccgg
     2281 cggcgtccag gcgggcgccc agctcggcaa agcgcgccgg ccggaacgag ggcaggatgt
     2341 cgggcgccag caggcggtcc aggtcggcct cgggcgccag ttgcaccggg taatgcgtgg
     2401 gctggccggg ctgccagccg tagccctgga agtagtgcgc caccgagccg atggcgtcgc
     2461 cttcgctgcc ccacaggtcg atgtggccgt cggcgtcgaa gtcgatggcg tagcgcaccc
     2521 agctggaggg catgaactgc ggcatgccca tggcgccggc gtagctgccc agcggctgct
     2581 gcgggtccat gccgctgcgc tgacacagcg acaggtactg cgccagctcg tcctgaaaga
     2641 acgcctgacg cgccgccgcc cgcgggtgct ggggcgggaa gtcgaagctg agcgtggcca
     2701 gcgcgtcgag cacgcgaaag ctgcccatgt tctggccgta gatggtttcc acgccgatga
     2761 tgccgaccac gatctcgggc ggcacgccgt agtcctgctg ggcgcgcgcc agcgtcgcgc
     2821 ggttgcgctg ccagaagcgg gcgccggcgc ggatgcgcgt ggcatcgatg aagcggctgc
     2881 ggtaggcgcg ccagttcttg gccgtgccgc gcgccggcgg cagcatcagg cgcggcacgt
     2941 tgggcaggtg ctgggcctgg ccgatggtgt cgcgcaccca gtcgggcggc aggtcgcgcc
     3001 gggccgccat gtcttcggcc cagcgcaggg cgtcggggcg gctggcgtag ctggacgggg
     3061 cggcctcggg caggcgacgg cgtttggggc cgttctgggc tgcagcgctg acggggagtg
     3121 cgccagcagc tatgcaaatg gtagcaagca ggcgacgtcg atcaaagggc aggcggggtg
     3181 cggtcatggc gggtggggtg gcgcggccag ggcaggcggc gcagttcgcg ttgcagtgta
     3241 gtcagccggg gcgccgccgc ctgcgacggg gcgtaacgca gggtttccag ggccagcagc
     3301 cagtcggtga gcggctggcg cagggccgcg ggcagcgtgc tgtgcgccac ctgctgcgcc
     3361 agcgcgcgtg gcggcgcctg cggcggcgca ttcaggccgg cctgggccag cagccggcgc
     3421 gcgcgcgcca gcaggcgcag ccaggggtcg tgctggctgc gttcccagcg cgcccaggcc
     3481 atgccgagca gggcggcggc caccagcagg ccagccagca ccttgcccag gtcggtccag
     3541 ttggggctgg caaagcccag ctggcgcagc aggtcgagct gccggctctg ggtgtagttg
     3601 agcacccatt ggttccagcc gttgttggcc gcttcccaca ggctgcgcag ctgcgccatc
     3661 agggtcgggc tgagggtgcc gagggcgccg gccaccaggc cttccgggcg gcgcagccgc
     3721 tgcagctggc cgatgcgcgc cggctgcacg gcggccgtgg ggtccacgcg cagccagccg
     3781 cgcccggggt cccagacttc ggtccaggcg tgggcgtcgg cgttgcgcac cacccagtag
     3841 ccgtcg
//
LOCUS       DAOOOG010000216         3811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_29032_length_3811_cov_28.057508, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000216 DAOOOG010000000
VERSION     DAOOOG010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29032_length_3811_cov_28.057508"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="PK242_12195"
     CDS             complement(<1..1132)
                     /locus_tag="PK242_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HOZ94821.1"
                     /translation="MSNLPGLNFQLGDDIDALRDAVRDFAQAEIAPRAAQIDRDNQFP
                     PDLWPKLGALGLHGMTVKEAFGGTEMGYLAHIVAMEEVSRASASVGLSYGAHSNLCVN
                     QIHRNGSEAQKKKYLPRLVSGEHVGALAMSEPNAGSDVVSMKLRAEKKNGYYVLNGGK
                     MWITNGGDADTLVIYAKTEPELGARGMTAFIVEKGFKGFSAGAKLDKLGMRGSNTFPL
                     YFDNCEVPEENVLGGEGNGAKVLMSGLDYERAVLSGGPLGIMAACMDAVVPYVHERKQ
                     FGQSIGEFQLMQGKIADMYSTWQATRAYVYAVGKACDSADHARSFRKDAAGAILYSAE
                     KATWMAGEAIQALGGVGYTRDFPVERLWRDAKLYEIGAGTSEI"
     gene            complement(1160..1603)
                     /locus_tag="PK242_12200"
     CDS             complement(1160..1603)
                     /locus_tag="PK242_12200"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOZ94822.1"
                     /translation="MTDAADPSAIFQRVAESFARQGMMRHLGAELRRVEAGRVEIWLP
                     YSDRVTQQQDGFHGGAMGAVADIAGGYAGLTLAPEGMEVVTAEYKINFLGSYRGGALR
                     AIGRVVKPGRRLIVTSAEVTHIDDAGQEGPCALMQQTLVPVPKQY"
     gene            complement(1640..2065)
                     /locus_tag="PK242_12205"
     CDS             complement(1640..2065)
                     /locus_tag="PK242_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOZ94823.1"
                     /translation="MATTYRISDLAREFDLTPRAMRFYEDLGLLRPERTGPGGRQRVY
                     SSRDRTRLKLTLRAKRLGLSLTEARELIDMYDSPRDTGPQLSRYLQVLGQHRAQLEAR
                     LAELQATLAEVAEQEQEARALLARLPGQGNRRPRRDAAD"
     gene            2189..3268
                     /locus_tag="PK242_12210"
     CDS             2189..3268
                     /locus_tag="PK242_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ94824.1"
                     /translation="MSLAAELERALHYPLGDQLPAPGCAVELRPGVKWLRMGLPFALD
                     HINLWLLRDRFQGREGWTVVDCCIDRPESRALWQQVFEQELEGLPVLRVLVTHMHPDH
                     IGLAHWLCERWSTPEHDCRLWISGADYQAARYGCEVVNSFGGERLADYFRSHGMTDPG
                     DLEQVRHRKSYYRGLVPAVPPQYARLRDGQPLVIGGHAWRCIAGWGHAPEHMALHCEA
                     LGILISGDMVLPRISTNVSVYEMEPEADALTLFLDSIDRFMPLPEDTLVLPSHGKPFQ
                     GLHTRIQQLHDHHRERLVEVMQAARERPVSAYDVLPVLFRRPLDLHQTTFALGESVAH
                     LHRLWHGGQLARTRDAEGIWRFAPV"
     gene            3369..>3811
                     /locus_tag="PK242_12215"
     CDS             3369..>3811
                     /locus_tag="PK242_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ94825.1"
                     /translation="MNTQQLFSLEGRSALVTGGSRGIGRMIAEGFLRAGARVYISARK
                     AEACDATARELSAIGPCVSLPADVSTLAGIQQLVAAYQKHEPSLDILVNNAGAAWGAP
                     YAEFPESGWDKVADLNMKTPFFLTQALTPMLKAAATDHLAKVIHIA"
BASE COUNT          557 a         1323 c         1291 g          640 t
ORIGIN      
        1 ggatctcgct ggtgccggcg ccgatctcgt acagcttggc gtcgcgccac aggcgctcga
       61 ccggaaagtc ccgcgtgtag ccgacgccgc ccagcgcctg tatggcttca ccggccatcc
      121 aggtggcctt ttcggccgag tacaggatgg cgccggcggc gtccttgcga aagctgcgcg
      181 cgtggtcggc actgtcgcag gccttgccca cggcgtagac gtaggcgcgc gtggcctgcc
      241 aggtgctgta catgtcggcg atcttgccct gcatgagctg gaactcgccg atgctctggc
      301 cgaactgctt gcgctcgtgc acgtagggca cgacggcgtc catgcaggcg gccatgatgc
      361 ccagcggccc gcccgacagc accgcgcgtt cgtagtccag cccgctcatc agcaccttgg
      421 cgccgttgcc ttcgccgccc agcacgtttt cctcgggcac ctcgcagttg tcgaaataca
      481 gcgggaaggt gttgctgccg cgcatgccca gcttgtcgag cttggcgccg gccgaaaagc
      541 ccttgaagcc cttctcgacg atgaaggccg tcatgccgcg cgcgcccagt tcgggctcgg
      601 tcttggcgta gatcaccagg gtgtcggcgt cgccgccgtt ggtgatccac atcttgccgc
      661 cgttcagcac gtagtagccg ttcttcttct cggcgcgcag cttcatgctg accacgtcgc
      721 tgccggcgtt cggctcgctc atggccagcg ccccgacatg ctcgccgctc accagcctgg
      781 gcaggtattt cttcttctgc gcttcgctgc cgttgcggtg gatctggttg acgcacaggt
      841 tggagtgcgc accgtaggac agaccgaccg aggccgaggc gcgcgagacc tcttccatgg
      901 ccacgatgtg ggccagatag cccatctcgg tgccgccgaa cgcttccttc acggtcatgc
      961 cgtgcaggcc cagcgcaccc agcttgggcc acaggtcggg cggaaactgg ttgtcgcggt
     1021 cgatctgggc ggcgcgcggg gcgatttcgg cctgcgcgaa gtcgcgcacg gcgtcgcgca
     1081 gcgcgtcgat gtcgtcacca agctgaaagt tcaggccggg caggttgctc atggggtctc
     1141 cttgacaaga tggatgggtt cagtactgct tgggcacggg caccagggtc tgctgcatca
     1201 gggcgcaggg gccttcctgg ccggcgtcgt cgatgtgtgt gacttcggcg ctggtgacga
     1261 tcaggcgccg gccgggtttg accacgcgcc cgatggcgcg caacgcgccg ccccggtagc
     1321 tgccgagaaa gttgatcttg tattcggccg tgaccacttc catgccctcc ggcgccagcg
     1381 tcaggccggc gtagccgccg gcgatgtcgg ccaccgcgcc catggcgccg ccgtgaaagc
     1441 cgtcctgctg ctgcgtcacg cggtcggaat acggcagcca gatctcgacc cgcccggcct
     1501 cgacccggcg cagctcggca cccaggtggc gcatcatgcc ctgacgggca aagctctcgg
     1561 ccacgcgctg gaagatggcg gaagggtcgg cggcgtcggt catggcaaag ctcgattgtg
     1621 attgacgttt acgtaaacgt caatcagcgg cgtctcggcg cggcctgcgg ttgccctgcc
     1681 ccggcaggcg cgccagcagc gcgcgcgcct cctgctcctg ctcggccacc tcggccagcg
     1741 tggcctgcaa ctcggccagc cgtgcctcca gctgggcgcg gtgctggccc agcacctgca
     1801 ggtaccggct cagctgcggg ccggtgtcgc gcgggctgtc gtacatgtcg atcagctcac
     1861 gcgcctcggt cagcgacagc cccaggcgct tggcgcgcag cgtcagcttc aggcgtgtgc
     1921 ggtcgcggct ggaatagacg cgctgacggc cgccggggcc ggtgcgctcg ggccgcagca
     1981 gacccaggtc ctcgtagaag cgcatcgcgc gcggcgtcag atcgaactcg cgtgccagat
     2041 cgctgatgcg gtaggtggtg gccatgggtg tcgggggctg tcgcgcaggc gccgcgtgcg
     2101 gcgcgactcc ttcatagaat ggtccggccg gttgacgtgc gcgtcggtcg tgcccgtcat
     2161 cctaacgcag ccgcctgctc cccagcccat gtcacttgcc gccgaactcg aacgtgccct
     2221 gcactacccg ctgggtgacc agctgcccgc gcctgggtgc gccgtcgaac tgaggccggg
     2281 cgtcaaatgg ctgcgcatgg gcctgccgtt cgcgctcgac cacatcaacc tttggctgct
     2341 gcgcgaccgc tttcagggcc gcgaaggctg gaccgtggtg gactgctgca tcgaccggcc
     2401 cgaatcgcgc gcgctgtggc agcaggtgtt cgagcaggag ctggagggcc tgccggtgct
     2461 gcgcgtgttg gtcacccaca tgcatcccga ccacatcggg ctggcgcact ggttgtgcga
     2521 gcgctggagc acgcccgaac acgactgccg cctgtggatc agcggcgccg actaccaggc
     2581 cgcgcgctac ggctgcgagg tggtcaacag ctttggcggc gagcgtctgg ccgactactt
     2641 ccgcagccac ggcatgaccg acccgggcga cctggagcag gtgcgccacc gcaagagcta
     2701 ctaccgcggc ctggtgccgg cggtgccgcc gcagtacgcg cgcctgcgcg acggccagcc
     2761 gctggtcatc ggcggccacg cctggcgctg catcgccggc tggggccacg cgcccgaaca
     2821 catggcgctg cactgcgagg cgctgggcat cctgatcagc ggcgacatgg tgctgccacg
     2881 catctcgacc aacgtcagcg tctatgaaat ggagcccgag gccgatgcgc tgacgctgtt
     2941 tctggactcc atcgaccgtt tcatgccgct gcccgaggac acgctggtgc tgccctcgca
     3001 cggcaagccc ttccagggct tgcacacgcg catccagcag ctgcacgacc accaccgcga
     3061 gcgcctggtc gaggtgatgc aggcggcgcg cgagcggccc gtgagcgcct acgacgtgct
     3121 gccggtgctg ttcaggcgcc cgctggacct gcaccagacc acctttgccc tgggcgagtc
     3181 ggtggcgcac ctgcaccgtc tgtggcacgg tggccagctg gcgcgcacac gcgacgccga
     3241 gggcatctgg cgctttgcgc cggtgtgaga ctcggtacgc cgaaccacca ccaccggagg
     3301 cgaaggactc gaactcaaag tccgacagcc tcccagacgc agccctaccg tatggagaac
     3361 ccggcatgat gaacacacag cagctctttt ccctggaggg ccgcagcgcc ctggtcaccg
     3421 gcggctcgcg cggcattggg cgcatgatcg ccgagggttt cttgcgcgcg ggcgcacggg
     3481 tctacatttc ggcgcgcaag gccgaggcgt gcgatgccac ggcgcgcgag ctatcggcca
     3541 tcgggccctg cgtgtcgctg ccggccgacg tgtccacgct ggcgggcatc cagcaattgg
     3601 tcgcggcgta ccagaagcac gagcccagcc tggacatcct ggtcaacaac gctggtgcgg
     3661 cctggggcgc gccgtatgcc gagttcccgg aaagcggctg ggacaaggtg gccgacctga
     3721 acatgaagac gccgttcttt ctcacgcaag cgctcacccc catgctcaag gccgccgcca
     3781 ccgaccacct ggccaaggtg atccacatcg c
//
LOCUS       DAOOOG010000217         3792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_29206_length_3792_cov_30.353492, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000217 DAOOOG010000000
VERSION     DAOOOG010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29206_length_3792_cov_30.353492"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PK242_12220"
     CDS             <1..509
                     /locus_tag="PK242_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348231.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOZ94826.1"
                     /translation="GADKPVLGICRGCQLLNVYFGGTLVQDIPSQWPGAIAHSDTAHY
                     DRLAHEVHFMPGSRLADIYGYEPRRVTSIHHQCVDRLGRDLVLEARSPIDLVPEGIRH
                     TGYPFVLGVQWHPEFHLNGNGAAADILDSGPLMMAFLKAAARRAGRVRRLAEGVARVR
                     ERASALTR"
     gene            528..1562
                     /gene="thiL"
                     /locus_tag="PK242_12225"
     CDS             528..1562
                     /gene="thiL"
                     /locus_tag="PK242_12225"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOZ94827.1"
                     /translation="MGEFELIDRHFKPKPAAARLHQATAAPDSIATPVVLGIGDDCAL
                     LQPAPGMQLAISTDMLVESRHFLPDVAPARLGHKALAVNLSDLAACGAEPLACTLALA
                     LPPERARDDAWLAALAGGLWALADAQRCPLVGGDTTAGPLNLCLTVFGQVPAGQALRR
                     DGARVGDDVWVSGALGHARLALGALRGEWTLPPQLLAAARTRLECPTPRIALGLALRG
                     IAHSAADVSDGLAGDLGHILRASAVGACLDAQETMKTIAAGAFSASAEGLFDRQTLLR
                     LALTGGDDYELVFTAPPDARSAVQAAGQASATPLARIGRIEAEPGLRLLGADGRIEPL
                     AARAFDHFAP"
     gene            1684..3288
                     /locus_tag="PK242_12230"
     CDS             1684..3288
                     /locus_tag="PK242_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOZ94828.1"
                     /translation="MTLIRYLCPSALAALWLALAPPAAAQGAAPASGGSRITAVKLYP
                     GSATVQRTLRLAAGARQALFACLPPGLDATSLQLSADAGVRVGELALRQQPRELLGPA
                     CASALDERIRTLEDRIAALAAETGGIDIATGYLKNFGAGGEPGRGTPAGQIGATAQAL
                     QQGGQTALLRRHQLARQQEQLERELKPLLAERDRASRGDQPVSTVQVTLWAPQGGELR
                     LSYQVRGPGWQPAYRASLDSASRQLRLERLAQVAQSSGEDWSEVPLTLSTGQPGAATQ
                     GPLPRPWRIGIEPPPAAGEARQRLAAAPAPVPVAAAAKAPEAEPSFDVSVFQGSFATE
                     FVLPQRVSVPSSGERITLTLGEQVLDTRLRVRTTPALDAHAYLIASFTTPAGVWPAGP
                     LHLVRDGAFVGSARFDAASASRDGLAFGRDELVGVRVERPEQQQGTGGFIGSRHQRID
                     SRRYVIDNRHREPIVLELLDSAPVGEHADVRIESRYQPAPQTERWNEQKGVVAWEQSL
                     AAGASQSFSAEHVISWPSDAHLRERR"
     gene            complement(3449..>3792)
                     /locus_tag="PK242_12235"
     CDS             complement(3449..>3792)
                     /locus_tag="PK242_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HOZ94829.1"
                     /translation="PQAALKLWDVAEVYAGEPFAQGASEDVRSLASSLGVPYCGVNAG
                     FDAVQWLDDPEAAASLALIPLRAGAAGPAFGLLILASPDSQRYQAGMATDFLERIAEL
                     ASAALSRVRDD"
BASE COUNT          526 a         1461 c         1345 g          460 t
ORIGIN      
        1 tcggcgccga caagccggtg ctgggcatct gccgcggctg ccagctgctc aacgtgtact
       61 ttggcggcac gctggtgcag gacatcccca gccagtggcc gggcgccatc gcccacagcg
      121 acaccgccca ttacgaccgt ctggcgcatg aggtgcactt catgcccggc tcgcggctgg
      181 ccgacatcta cggctacgaa ccgcgccgcg tcaccagcat ccaccaccag tgcgtcgacc
      241 gcctgggccg cgacctggtg ctggaggcgc gcagcccgat cgacctggtg cccgagggca
      301 tccgccacac cggctacccg ttcgtgctgg gcgtgcagtg gcaccccgag ttccatctca
      361 acggcaacgg cgccgccgcc gacatcctgg acagcggccc gctgatgatg gccttcctga
      421 aggccgccgc acgccgcgcc ggccgcgtgc gccggctggc cgagggcgtg gcccgcgtgc
      481 gcgaacgcgc cagcgcgctc acccgctgag cgcgcgcagg agccaccatg ggcgaattcg
      541 agctgatcga tcgtcatttc aagccaaaac cggctgcagc gcgcttgcat caagcgacag
      601 cagctcctga ttcaatagca acgcccgtgg tgctggggat cggcgacgac tgcgccctgc
      661 tgcagccggc gcccggcatg caactggcca tcagcaccga catgctggtc gagagccgcc
      721 acttcctgcc cgacgtcgcg ccggcccggc tgggccacaa ggcgctggcg gtcaacctga
      781 gcgacctggc ggcctgcggc gccgagccgc tggcctgcac gctggcgctg gcgctgccgc
      841 ccgagcgcgc gcgcgacgac gcctggctgg cggccctggc cggcggcctg tgggccctgg
      901 ccgacgccca gcgctgcccg ctggtgggcg gcgacaccac ggccggcccg ctcaacctgt
      961 gcctgacggt gttcggccag gtgccggccg ggcaggcgct gcggcgcgac ggcgcgcgcg
     1021 tgggcgacga cgtctgggtc agcggcgcgc tgggccacgc ccggctggcc ctgggcgcgc
     1081 tgcgcggcga atggacgctg ccgccgcagc tgctggccgc cgcgcgcacg cggctcgagt
     1141 gccccacgcc ccgcatcgcg ctcggcctgg cgctgcgcgg catcgcccac agcgccgccg
     1201 acgtcagtga cgggctggcg ggcgatctgg gccacatcct gcgggcgagc gccgtcgggg
     1261 cctgcctgga cgcccaggaa actatgaaaa ccatagctgc tggcgctttc tcggcaagcg
     1321 ctgaaggcct ttttgatcgt caaaccctgc tgcgcctggc gctcaccggt ggcgacgatt
     1381 acgagctggt cttcaccgcc ccgcccgacg cccgcagcgc cgtgcaagcc gccggccagg
     1441 ccagcgccac cccgctcgcg cgcattggcc gcatcgaagc cgaacccggc ctgcgcctgc
     1501 tcggcgccga cggtcgcatc gaaccgctgg ccgcgcgcgc cttcgaccat ttcgcccctt
     1561 gactgagcct ttggatgtct tgaataccgt tcgggccgag ctcgtcgaag cccatcgaca
     1621 ggctcagggt gagcggttcc atgttccaga cagggagcca taccacaccg catgaaccgc
     1681 accatgaccc tgatccgata cctttgccca tccgccctgg ccgcgctgtg gctggccctg
     1741 gccccgccgg ccgccgccca gggcgccgcc ccggccagcg gcggctcgcg catcaccgcc
     1801 gtcaagctgt accccggcag cgccaccgtc cagcgcacgc tgcgcctggc ggcgggcgcg
     1861 cgtcaggcgc tgtttgcctg cctgccgccg gggctggacg ccaccagcct gcagctcagc
     1921 gccgacgccg gcgtgcgcgt gggtgagctg gcgctgcgcc agcagccgcg cgagctgctg
     1981 ggcccggcct gcgccagcgc gctggacgaa cgcatccgca cgctggaaga ccgcatcgcc
     2041 gcgctggcgg ccgaaaccgg cggcatcgac atcgccaccg gctacctgaa gaacttcggc
     2101 gccggcggcg agccggggcg cggcacgccc gccggccaga tcggcgccac ggcgcaggcc
     2161 ctgcagcagg gcggccagac ggcgctgctg cggcgccacc agctggcgcg ccagcaggag
     2221 cagctggagc gcgagctcaa gcccctgctg gccgaacgcg accgcgccag ccggggcgac
     2281 cagccggtca gcacggtgca ggtcacgctg tgggcgccgc agggcggtga gctgcgcctg
     2341 agctaccagg tgcgcggccc cggctggcaa cccgcctacc gcgccagcct cgacagcgcc
     2401 agccgccagc tgcgcctgga gcgcctggcg caggtggcgc aaagcagcgg cgaggactgg
     2461 agcgaggtgc cgctcaccct gtccaccggt cagcccggcg ccgccaccca ggggccgctg
     2521 ccgcggccct ggcggatcgg catcgagccg ccgccggcgg ccggcgaagc gcgccagcgc
     2581 ctggccgcgg cccctgcccc ggtgccggtg gcggccgcgg ccaaggcgcc cgaggccgag
     2641 ccgagtttcg acgtcagcgt gttccagggc agcttcgcca ccgaattcgt gctgccgcag
     2701 cgcgtcagcg tgccctccag cggcgagcgc atcacgttga cgctgggcga gcaggtgctg
     2761 gacacccggc tgcgcgtgcg caccacgccg gcgctggacg cccacgccta cctgatcgcc
     2821 agcttcacca ccccggccgg cgtttggccg gccggcccgc tgcacctggt gcgcgacggc
     2881 gccttcgtcg gcagcgcccg cttcgacgcc gccagcgcca gccgcgacgg tctggcgttc
     2941 gggcgcgacg aactggtcgg cgtgcgcgtc gagcggcccg agcagcagca gggcacgggc
     3001 ggcttcatcg gcagccgcca tcagcgcatc gacagccgcc gctacgtgat cgacaaccgc
     3061 caccgcgagc ccatcgtgct ggaactgctg gacagcgccc cggtgggcga gcacgcggac
     3121 gtgcggatcg aatcgcgcta ccagccggcg ccgcagaccg agcgctggaa cgagcagaaa
     3181 ggcgtggtgg cctgggagca gtcgctggcc gccggcgcca gccagagctt cagcgccgag
     3241 cacgtcatca gctggcccag cgatgcgcac ctgcgtgagc ggcgctgaga tggggccggt
     3301 tcagtcgagg gaccgatgac agtggacacc ctcgatcatc caagcgccgc caggtcatcg
     3361 gcagcagccg tcgccgatgg cgcggcgcgc gcaccgggcc gctgaaggcc agcgaagcca
     3421 ccaggccctg ccaccgcggc ttgccggctc aatcgtcgcg cacccgcgac agcgccgcgc
     3481 tggcgagctc ggcgatgcgc tccaggaagt cggtggccat gccggcctgg tagcgttggc
     3541 tgtcgggcga ggctaggatc agcaggccga acgccggacc ggcggcgccg gcgcgcagcg
     3601 ggatcagcgc cagcgaggcg gcggcctcgg gatcgtccag ccactgcacg gcgtcgaagc
     3661 cggcgttgac gccgcaatac ggcacgccga gcgacgaggc caggctgcgc acgtcctcgc
     3721 tggcgccctg cgcgaacggc tcgccggcat agacctcggc cacgtcccac agcttgagcg
     3781 ccgcctgcgg ca
//
LOCUS       DAOOOG010000218         3674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_30492_length_3674_cov_24.781984, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000218 DAOOOG010000000
VERSION     DAOOOG010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30492_length_3674_cov_24.781984"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..963)
                     /locus_tag="PK242_12240"
     CDS             complement(64..963)
                     /locus_tag="PK242_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ94830.1"
                     /translation="MDKFQEMRVFVAVVDAGSFVRAADALDLSKAAVSRLVGDLEARL
                     GVRLLHRTTRKLSLTEVGEVFHERCRQLLDSVAEAEAELSTHSGAVIGRLKINVPFTF
                     GLLHLASLWPRFMAQHPQVVLDVTLSDRFVDLVDEGYDLAVRIARLQASSLVSRRLAS
                     THMVLCASPQYLREHGAPVHPAELEQHALISYTLLSTGEQWELQGPEGSLNVRISPRM
                     RTNSGDTCCAAAIGHQGIVFQPTFLVAQHLASGALVPVLPQYRSPEIGIYAVYPTRKH
                     LTPKVRALIDFLAEALRTPAWSG"
     gene            1084..1716
                     /locus_tag="PK242_12245"
     CDS             1084..1716
                     /locus_tag="PK242_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOZ94831.1"
                     /translation="MKVTVIGAGHMGSAFVKQLVQAGHEVSVTARSRAKAERLAAAHP
                     GAKAVPAATAAAGADVIVLATGYGDAVEALKSVGDLQGKVVIDITNPLTADYMGLTLG
                     HSTSAAEEIAKAVPGAQIVKGFNTVFAQVLAEGADFGAGRSVTVFVASDSERARQTAS
                     ALAHSMGFEVVDAGGLKNARYLEPLAGLNIYLGYGAGLGTAIAPTWIRKA"
     gene            1740..2186
                     /locus_tag="PK242_12250"
     CDS             1740..2186
                     /locus_tag="PK242_12250"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOZ94832.1"
                     /translation="MSESLIQRLLATGNGSAALVLRVPVGIIFAAHGAQKLFGWFGGH
                     GLEGTGQFFASIGLAPGYLMALLAGAAEFFGGLALIVGLLVRPAGAALAFAMLVAIVS
                     AHGGKGFFLASGGLEYALALLAAALSLAFSGAGRCSLDRAFARSRS"
     gene            complement(2245..2595)
                     /gene="cutA"
                     /locus_tag="PK242_12255"
     CDS             complement(2245..2595)
                     /gene="cutA"
                     /locus_tag="PK242_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOZ94833.1"
                     /translation="MAAPLPWLVVTTVGSREQAQALARTLVHLRLAACVQIEAIDSIY
                     RWQGEVLEDGEFRLLCKTQPAQYAQLEAAIRQHHPYQLPAIHAIATVAADPAYAAWVA
                     DNAGGPAAPTSGRR"
     gene            complement(2610..>3674)
                     /locus_tag="PK242_12260"
     CDS             complement(2610..>3674)
                     /locus_tag="PK242_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOZ94834.1"
                     /translation="QGVMKDIAATLKQVYHADAAVIVPGSGTFGMEAVARQFATGQKV
                     LIVRNGFFSYRWSQILEMGQITAPDHIRVLKARRTADGPQAPWAPAPIDEVTAAIAHE
                     RPAVVFAPHVETASGMMLPDDYVRRLGEATRAVGGLFVLDCVASGAMWVDMQALGVDV
                     LISAPQKGWSSAPACAMVCLSERALQAMEGRTSTSYSCDLKKWHQIMRAYETGAHAYH
                     ATLPTMALLELRDAMAETRARGFAQVREQQLELGHKVRALLAARGFPSVAAPGFEAPG
                     VVVSYTRDPDIQNGRKFIEQGLQSASGVPLMCDEPADFSTFRLGLFGLDKWANVDRSV
                     GQLATALDRMGHPAPAGAPA"
BASE COUNT          541 a         1238 c         1291 g          604 t
ORIGIN      
        1 gcaggcttgc gcccggtgag gcccgcttgg cgcccggtgg gcaccctgtg gtctggcgcg
       61 gcctcagcct gaccacgccg gggtgcgcaa ggcttcggcc agaaagtcga tcagcgcgcg
      121 caccttgggc gtcaggtgct tgcgcgtcgg atagaccgca tagatgccga tctcgggcga
      181 gcggtattga ggcagcaccg gcaccagcgc gcccgaggcc aggtgctgcg ccaccagaaa
      241 ggtcggctgg aacacgatgc cctgatgccc gatcgcggcg gcgcagcagg tgtcgccgct
      301 gttggtgcgc atgcgcgggc tgatcctgac gttgaggctg ccttcgggcc cttgcagctc
      361 ccactgttcg ccggtggaca gcagcgtgta ggagatcagc gcgtgctgct ccaactccgc
      421 cgggtgcacg ggtgcgccgt gctcgcgcag gtattgtggt gacgcgcaca gcaccatgtg
      481 ggtgctggcc agccgccggc tgaccagcga tgaagcctgc agccgcgcaa tccgcaccgc
      541 caggtcgtag ccctcgtcga ccagatcgac gaagcggtcg gacagcgtga cgtcgagcac
      601 cacctggggg tgctgcgcca tgaagcgcgg ccacagcgac gccagatgca gcagcccgaa
      661 ggtgaacggc acgttgatct tcaggcgccc gatcaccgcg cccgagtggg tgctgagctc
      721 ggcctcggcc tcggccacgc tgtcgagcag ctgccggcag cgctcgtgaa acacctcgcc
      781 cacttcggtc agcgacagct tgcgcgtggt gcggtgcagc aggcgcacgc ccaggcgcgc
      841 ttccagatcg ccgaccaggc gcgacaccgc ggccttggac agatccagcg catcggccgc
      901 acgcacaaag ctgccggcgt ccaccacggc cacgaacacc cgcatttcct gaaacttgtc
      961 catcgcccat tgttttgatg atcggaacat tttgtcaatc gatcggcggt ttatcaaaga
     1021 ttctctgaac cataaagttc agtccatcgc agcgatccgc tgtatttcac tgaggaacag
     1081 atcatgaaag tcaccgtcat cggcgccggc cacatgggtt cggcattcgt caagcaactg
     1141 gtccaggccg gccacgaggt cagcgtcacg gcgcgcagcc gcgccaaggc cgagcggctc
     1201 gccgccgccc atccgggcgc caaggcggtg cctgccgcaa cggctgccgc cggcgcggat
     1261 gtcatcgtgc tggccacggg ctacggcgac gccgtggagg cactcaagtc cgtgggcgac
     1321 ctgcagggca aggtggtgat cgacatcacc aacccgctga cggccgacta catgggcctg
     1381 acgctgggtc acagcacttc ggctgccgag gaaatcgcca aggccgtgcc gggggcgcag
     1441 atcgtcaagg gcttcaacac cgtgtttgcc caggtgctgg ccgaaggcgc ggacttcggc
     1501 gccggccgca gcgtcacggt gttcgtcgcc agcgacagcg agcgtgccag gcagaccgcc
     1561 agcgcgctgg cgcacagcat gggcttcgag gtggtcgatg ccggcggcct gaagaacgcg
     1621 cgctacctgg agccgctggc gggcctgaac atctacctgg gctatggcgc cgggttgggc
     1681 acggccattg cgccgacctg gatccgcaag gcttgagcca ccccatccgg agacatctca
     1741 tgagcgaatc cctcatccaa cgcttgctgg ccacaggcaa cggcagcgct gccctggtcc
     1801 tgcgtgtccc ggtgggcatc atctttgcgg cgcatggcgc gcaaaagctg tttggctggt
     1861 tcggcggcca tggcctggaa ggtacgggcc agttcttcgc ctcgatcggg ctggcgccgg
     1921 gctatctcat ggccctgctg gcgggcgctg cggagttttt cggcgggctg gccttgatcg
     1981 tcggactgct ggtgcgcccg gccggcgcgg cgctggcctt cgccatgctg gtcgccatcg
     2041 tctcggcgca tgggggcaag ggcttctttc ttgccagcgg cgggctggag tacgcgctgg
     2101 cgctgctggc ggcggcgctg tcgctggcct tcagcggggc cgggcgctgc tcgctcgatc
     2161 gcgcctttgc acgctcacgg tcctgagcct ccaatatggc cctggcgctt gctgggcgtg
     2221 cgccaacagc tatacatacg atagctatct gcggcctgaa gtcggggccg ccgggccgcc
     2281 ggcgttgtcg gccacccagg cggcgtaggc ggggtcggcc gccacggtgg cgatggcgtg
     2341 gatcgccggc agctggtagg ggtggtgctg gcggatggcg gcttcaagct gcgcgtactg
     2401 ggccggctgg gtcttgcaca gcaggcgaaa ttcgccgtct tcgagcacct cgccctgcca
     2461 gcggtagatg ctgtcgatgg cttcgatctg cacgcaggcg gccagccgca ggtgcaccag
     2521 ggtgcgcgcc agcgcctgcg cctgctcgcg cgagccgacg gtggtgacca ccagccaggg
     2581 caagggcgcg gccatggccc ggcccgcctt caggctgggg cgcctgccgg cgccgggtgg
     2641 cccatgcggt cgagcgccgt cgccagctgg ccgacgctgc gatcgacgtt ggcccatttg
     2701 tcgaggccga acaggccgag acggaaggtg ctgaagtcgg ccggctcgtc acacatcagg
     2761 ggcaccccgg acgcgctttg caggccctgc tcgatgaact tcctgccgtt ctggatgtcc
     2821 gggtcgcggg tgtagctgac caccaccccc ggtgcttcaa agccgggcgc ggccacgctg
     2881 ggaaagccgc gcgccgccag cagcgcccgc accttgtggc ccagttcgag ttgctgctcg
     2941 cgcacctggg caaagccgcg cgcacgggtt tcggccatgg cgtcgcgcag ctccagcagc
     3001 gccatggtgg gcagcgtggc gtggtaggcg tgggcgccgg tttcgtaggc ccgcatgatc
     3061 tggtgccact tcttgaggtc gcacgaataa ctggtgctgg tgcggccttc catcgcctgc
     3121 agtgcgcgct ccgacaggca caccatggcg caggccgggg cgctgctcca gcccttctgt
     3181 ggcgcgctga tcagcacgtc gacgcccagg gcctgcatgt ccacccacat ggcgccggag
     3241 gccacgcagt cgagcacgaa caggccaccg acggcgcgcg tggcttcgcc cagccgccgg
     3301 acgtagtcgt cgggcagcat catgccgctg gcggtttcga catggggagc gaacaccacc
     3361 gccggccgtt cgtgcgcgat cgctgccgtc acttcgtcga tcggtgccgg cgcccagggg
     3421 gcttgcgggc cgtcggcggt gcggcgcgcc ttgagcacgc ggatgtgatc gggcgcggtg
     3481 atctgcccca tctccaggat ctggctccag cggtagctga agaagccgtt gcgcacgatc
     3541 aacaccttct gcccggtggc gaactggcgc gccaccgcct ccatgccgaa ggtgccgctg
     3601 ccgggcacga tgacggcggc gtcggcgtgg tagacctgct tgagcgtggc cgcgatgtcc
     3661 ttcatcacgc cctg
//
LOCUS       DAOOOG010000219         3667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_30576_length_3667_cov_29.197398, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000219 DAOOOG010000000
VERSION     DAOOOG010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30576_length_3667_cov_29.197398"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PK242_12265"
     CDS             <1..1300
                     /locus_tag="PK242_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOZ94835.1"
                     /translation="DLPVVLPQDLVPDGSGNPLNKCEAFLQVACPCCGQPARRETDTM
                     DTFVDSSWYFMRYCDPTNHRQMVAEGSRYWMPMDQYIGGIEHAILHLLYARFWTKVMR
                     DLGLVQIDEPFTRLLTQGMVLNHIYSRRNDKGGIEYFWPADVEPVFDADGHQVGARLN
                     KAVGALPAGTPIDYEGVGTMSKSKNNGVDPQQLIDRYGADTARLYTMFTAPPEATLEW
                     NDAAVEGSYRFLRRVWNFGVKLNATENIAARAVLASAAAQNDLKLSKAAKALRHEIHS
                     VLGQIDYDYQRLQYNTVVSGAMKMLNALEGFNPDAAERGDQAVLAEGFSKLLRVLYPV
                     TPHLTHVLWQELGFAAVHGDLLDAPWPRVDPEALRQDEIELVLQINGKLRGAVAVPAG
                     ADQARIEQLALASEAFARHASGTVRKVVVVPGRLVNIVVA"
     gene            1318..1818
                     /gene="lptE"
                     /locus_tag="PK242_12270"
     CDS             1318..1818
                     /gene="lptE"
                     /locus_tag="PK242_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOZ94836.1"
                     /translation="MRPTRRTLLLASVPLLLSGCGFALRQAPQFGFRSLYLALPAHSA
                     LALELRRQLEGSGSLQVFTDAAQRDKAEVVLESPGEQRERAVVSVSAGGEVREFQLRI
                     RLRFRLRAADGRELLPMSEILRQIDQSYSESAALSKEQEALMLYQNMQSDIVQQVMRR
                     LATVRL"
     gene            1820..2887
                     /gene="holA"
                     /locus_tag="PK242_12275"
     CDS             1820..2887
                     /gene="holA"
                     /locus_tag="PK242_12275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOZ94837.1"
                     /translation="MQLAAAQLARHLQQGLRPLYVLHGDEALLVQEAADAIRATARAQ
                     GHTERSVHTVAGAHFDWGAVLAAGSSLSLFADRQLVEIRIPSGKPGKDGSAALQQLAE
                     AAPGNDGVLTLVLLPRLDKATRTSAWFAALDGAGVTVQIDGIERAALPLWLAQRLAAQ
                     GQRVAAGEEGQRTLAFFADRVEGNLLAAHQEISKLALLHPPGELSWAQVEAAVLNVAR
                     YDVFKLSEAVLAGQPARVQRMLDGLQAEGEAEVLVHWALAEDIRALQRVRDALDAGKP
                     LPMALREHRIWGPRERLFERVLPRLSAATASRLLHSAHVVDGIVKGLKAPDWPHDGWQ
                     ALHRLAMQLCRACMPAAPRPR"
     gene            2953..>3667
                     /locus_tag="PK242_12280"
     CDS             2953..>3667
                     /locus_tag="PK242_12280"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOZ94838.1"
                     /translation="MNALSIADHIHTLGLQARTASAQMARAPAAIKNKALRALARRLR
                     DGVQALQTDNAQDLERAVAAGLSAPMVDRLRLTPQVIETCAQGCEQLAAMPDVIGDIV
                     GMRQQPSGIRVGQMRVPIGVFGMIYESRPNVTIEAASLSIKSGNACILRGGSEAIASN
                     QALARLVVQALQDAGLPAHGVQLIQTTDRAAVGQLIAMPQYVDVIIPRGGKGLIERIA
                     AEAKVPVIKHLDGNCHSYVD"
BASE COUNT          582 a         1315 c         1275 g          495 t
ORIGIN      
        1 ggacctgccg gtggtgctgc cgcaggacct ggtgcccgac ggcagcggca acccgttgaa
       61 caagtgcgaa gccttcctgc aggtggcctg cccctgctgc ggccagccgg cgcggcgcga
      121 aaccgacacc atggacacct tcgtcgattc gtcctggtac ttcatgcgct actgcgaccc
      181 gaccaaccac cgccagatgg tggccgaagg cagccggtac tggatgccca tggaccagta
      241 catcggcggc atcgagcacg ccatcctgca cctgctgtac gcacgtttct ggaccaaggt
      301 gatgcgcgat ctggggctgg tgcagatcga cgagcccttc acgcgcctgc tgacgcaggg
      361 catggtgctc aaccacatct attcgcgccg caacgacaag ggcggcatcg aatacttctg
      421 gccggccgac gtcgagccgg tgttcgacgc cgacggccac caggtcggcg ccaggctgaa
      481 caaggccgtg ggcgccctgc cggccggcac gccgatcgac tacgagggcg tgggcaccat
      541 gtccaagtcc aagaacaacg gcgtcgatcc gcagcagctg atcgaccgct acggcgccga
      601 caccgcgcgc ctgtacacca tgttcaccgc cccgcccgag gccacgctgg agtggaacga
      661 cgccgcggtg gaaggcagct accgcttcct gcgccgggtg tggaacttcg gcgtcaagct
      721 gaatgctact gaaaacatag ctgctcgcgc agttctggca agcgctgcgg cccagaatga
      781 cctgaaactg agcaaggccg ccaaggccct gcggcacgag atccacagcg tgctggggca
      841 gatcgactac gactaccagc gcctgcagta caacaccgtg gtgtcgggcg ccatgaagat
      901 gctcaacgcg ctggaaggct tcaaccccga cgccgccgaa cgcggcgacc aggcggtgct
      961 ggccgaaggc ttttccaagc tgctgcgcgt gctctacccg gtcacgccgc acctcacgca
     1021 cgtgctgtgg caggagctcg gctttgccgc ggtgcatggc gacctgctgg acgcgccctg
     1081 gccgcgcgtc gacccggagg cgctcaggca ggacgaaatc gagttggtgc tgcagatcaa
     1141 cggcaagctg cgcggcgccg tcgccgtgcc cgccggcgcc gaccaggccc ggatcgagca
     1201 actggcgctg gccagcgaag cctttgcgcg ccacgccagc ggcacggtgc gcaaggtggt
     1261 ggtggtgccc ggccggctgg tcaacatcgt ggtggcctga gaggcggtga cagcgccatg
     1321 cgccccacac gccgtaccct gctgctggcc agcgtaccgc tgctgctgag cggctgcggc
     1381 tttgcgctgc ggcaggcgcc gcagttcggc tttcgcagcc tgtacctggc gctgccggcc
     1441 cattcggcgc tggcgctgga gctgcgccgc cagctcgaag gcagcggcag cctgcaggtc
     1501 ttcaccgacg ccgcgcagcg cgacaaggcc gaggtggtgc tggaaagccc gggcgagcag
     1561 cgcgagcgcg ccgtcgtcag cgtgtcggcc ggcggcgagg tgcgcgaatt ccagctgcgc
     1621 atccgcctgc gctttcgcct gcgcgccgcc gacgggcgcg agctgctgcc gatgtcggaa
     1681 atcctgcgcc agatcgacca gagctacagc gaatcggccg cgctgtccaa ggaacaggag
     1741 gcgctgatgc tctaccagaa catgcagagc gacatcgtgc agcaggtcat gcggcggctg
     1801 gcgaccgtga ggctctgaca tgcagctggc ggccgcgcaa ctggcccggc acctgcagca
     1861 gggcctgcgc ccgctgtacg tgctgcacgg cgacgaggcc ctgctggtgc aggaggcggc
     1921 cgacgccatc cgcgccaccg cgcgcgccca gggccacacc gagcgcagcg tgcacaccgt
     1981 cgccggcgcg catttcgact ggggcgcggt gctggccgcc ggcagcagcc tgagcctgtt
     2041 tgccgaccgg cagctggtcg aaatccgcat cccctcgggc aagccgggca aggacggcag
     2101 cgcggcgctg cagcagctgg ccgaggccgc gcccggcaac gacggcgtgc tgaccctggt
     2161 gctgctgccg cggctggaca aggccacccg caccagcgcc tggtttgcgg cgctcgacgg
     2221 cgccggcgtg acggtgcaga tcgacggcat cgagcgcgcc gcgctgccgc tgtggctggc
     2281 ccagcgcctg gccgcccagg gccagcgcgt ggcggcgggc gaggaaggcc agcgcaccct
     2341 ggccttcttt gccgatcggg tggaaggcaa cctgctggcc gcgcaccagg aaatcagcaa
     2401 gctggccctg ctgcacccgc cgggcgagct gtcctgggcc caggtcgagg ccgccgtgct
     2461 gaacgtggcg cgctacgacg tgttcaagct gtccgaggcg gtgctggccg gccagccggc
     2521 gcgcgtgcag cgcatgctcg acggcctgca ggccgagggc gaggccgagg tgctggtgca
     2581 ctgggcgctg gccgaagaca tccgcgccct gcagcgcgtg cgcgacgcgc tggacgccgg
     2641 caagccgctg ccgatggcgc tgcgcgaaca ccgcatctgg ggcccgcgcg agcgcctgtt
     2701 cgagcgcgtg ctgccgcgcc tgagcgccgc caccgccagc cgcctgctgc actcggccca
     2761 tgtggtggac ggcatcgtca agggcctgaa ggcgcccgac tggccgcacg acggctggca
     2821 ggccctgcat cggctggcga tgcagctgtg ccgcgcctgc atgcctgccg cgccgcgccc
     2881 gcgctgaacc ggcgcgcgca ccgcgggcgc cgcgccgccg ccctcacacc ccatctgccg
     2941 taccatcaca ccatgaacgc gctttccatc gccgaccaca tccacaccct tggtttgcag
     3001 gccagaacgg cttcggcgca gatggcgcgt gcgccggcag ctattaaaaa caaagcattg
     3061 cgggcgctgg cgcggcgcct gcgcgacggc gtgcaggcgc tgcagaccga caacgcccag
     3121 gacctggagc gcgccgtggc ggccggcctg agcgcgccca tggtcgatcg cctgcgcctg
     3181 accccgcagg tgatcgaaac ctgcgcccag ggctgcgagc agctggcggc catgcccgac
     3241 gtgatcggcg acatcgtggg catgcgccag cagcccagcg gcatccgcgt ggggcagatg
     3301 cgggtgccga tcggcgtgtt cggcatgatt tacgaaagcc gccccaacgt caccatcgag
     3361 gccgccagcc tgtccatcaa gagcggcaac gcctgcatcc tgcgcggcgg ctccgaggcc
     3421 atcgcctcca accaggcact ggcacggctg gtggtgcagg cgctgcagga cgccggcctg
     3481 cccgcgcacg gcgtgcagct gatccagacc accgaccgcg ccgccgtcgg ccagctgatc
     3541 gccatgccgc agtacgtgga cgtcatcatc ccgcgcggcg gcaaggggct gatcgagcgc
     3601 atcgccgccg aagccaaggt gcccgtcatc aagcacctgg acggcaactg ccacagctac
     3661 gtcgacg
//
LOCUS       DAOOOG010000220         3616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_31145_length_3616_cov_30.700646, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000220 DAOOOG010000000
VERSION     DAOOOG010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31145_length_3616_cov_30.700646"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..571)
                     /locus_tag="PK242_12285"
     CDS             complement(2..571)
                     /locus_tag="PK242_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HOZ94839.1"
                     /translation="MNNPHRRHFLGRSLGAAQLAVASVLLPAGGRALASAPDTLTPAR
                     RLAFNHLHTQERTALVYAVGEAFVPSALDHLNHFLRDHYSGDVGVIDPELFHLLHRVR
                     RELESEQPFHVISGYRSPHTNETLRTTRGGGVARRSLHMDGKAIDVRLPGVALADLRD
                     AALSLKAGGVGFYPRENFVHIDTGRVRSW"
     gene            complement(608..2161)
                     /locus_tag="PK242_12290"
     CDS             complement(608..2161)
                     /locus_tag="PK242_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOZ94840.1"
                     /translation="MLTRARLGAAAALLWLAPWAQAQPLWLDALGQPGPAAREAIGWL
                     TQAERDGLQPHDYDAARWARSIDPGQRLAATEAGAWDAALTQTLTRYLHELRHGRVPA
                     AALGARYDSVGQPAPDLAERLHDAVRNGRPAEALAAATPPWPQYPLLRTALAQYRALV
                     DDPAWRTALPPLSGDRLRPGQRWPGLAALAARLHALGDLDAAPATEPDTFDPALQHAV
                     KAFQERHGLSADGVLGKATLEALDVPPAARARQIALALERLRLTPLPAAERFVTVNVP
                     EFMLYAWQRQDQAVHQAFAMRVIVGKAVGTRTPLFDEDMRRIEFNPYWNIPPSIARGE
                     TLPKLRANPGYLAAQGMEFVGPGGQTSSAVTAELLDAVQAGQWRLRQRPGRLNALGDI
                     KFVLPNDQNIYLHHTPSVGLFQRARRDFSHGCIRIEQPVALAQWVLADDPDWSEARIR
                     QTMGQPRTVSVALPTPVPVLIVYRTVSVRDGRVHFAPDLYRQDALLERALAQRPHPAR
                     SPGPVRGAS"
     gene            complement(2187..3614)
                     /locus_tag="PK242_12295"
     CDS             complement(2187..3614)
                     /locus_tag="PK242_12295"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOZ94841.1"
                     /translation="MLKTDALINGKWVKGKARFDVSDPATGRKLADVADLGARETRSA
                     IQAANAAWPAWRELTGKQRHAHLMAWFQLLMQHQDDLARLMTAEQGKPLAEAKGEVAY
                     GASFVEWFAEEAKRVQGETLTPFDRNKRLTVIQQPIGVCAAITPWNFPLAMITRKVAP
                     ALAAGCTVVIKPAEFTPLTALAAAELAQRAGIPAGVINVVTGLDAPAIGRELCANDIV
                     RHLSFTGSTEVGRILMAQCAPTVKKLALELGGNAPFIVFDDADLDSAVEGALASKYRN
                     AGQTCVCANRLYVQDGVYDAFVKKLAARVKAMKVGNGFEDGVTVGPLIEPAALAKVEQ
                     HVKDAVRKGGKVLTGGRRLSGQFYEPTVIAGATAAMRVAREETFGPLAPVFRFRTEQE
                     AIDAANATEFGLASYFYARDVGRITRVAEGLEYGMVGINTGLISTEHVPFGGVKQSGL
                     GREGSRHGMAEYLEMKYLCLGDVQK"
BASE COUNT          454 a         1241 c         1385 g          536 t
ORIGIN      
        1 cctaccagct gcgcacgcgc ccggtgtcga tgtgcacgaa gttctcgcgc gggtagaaac
       61 ccacgccgcc ggccttgagc gacagggcgg cgtcgcgcag atcggccagc gccacgcccg
      121 gcaggcgcac gtcgatggcc ttgccgtcca tgtgcaggct gcgccgggcc acgccgccgc
      181 cgcgcgtggt gcgcagggtt tcgttggtgt gcgggctgcg gtagccggaa atgacgtgga
      241 agggctgctc gctttccagc tcgcgccgca cgcggtgcag caggtgaaac agctcggggt
      301 cgatcacgcc gacgtcgccg ctgtagtggt cgcgcaggaa gtggttcagg tggtccagcg
      361 cgctgggcac gaaggcctcg cccaccgcat agaccagcgc cgtgcgctcc tgcgtgtgca
      421 ggtggttgaa cgccaggcgg cgcgccggcg tcagggtgtc gggggcgctg gccagcgccc
      481 ggccgccggc cggcagcagc acgctggcca cggccagctg ggcggcgccc aggctgcggc
      541 ccagaaagtg gcgacgatga ggattgttca tgcgacggat tcctgaatcg ttgcaaacca
      601 aagcattcta agacgcgccg cgcacgggac ccgggctgcg ggcggggtgc ggtcgctgcg
      661 ccagcgcgcg ctccagcagc gcgtcctgac ggtacaggtc gggcgcaaag tgcacccggc
      721 cgtcgcgcac gctgaccgtg cggtagacga tcagcaccgg caccggcgtg ggcagcgcca
      781 cgctgaccgt gcgcggctgg cccatggtct ggcggatgcg cgcctcgctc cagtcggggt
      841 cgtccgccag cacccactgc gccagcgcca ccggctgctc gatgcggatg cagccgtggc
      901 tgaagtcgcg ccgggcgcgc tggaacaggc cgaccgaggg cgtgtggtgc aggtagatgt
      961 tctggtcgtt gggcagcacg aacttgatgt cgcccagcgc gttcagccgg cccgggcgct
     1021 ggcgcaggcg ccactggccg gcctgcaccg cgtccagcag ctcggctgtg acggcgctgc
     1081 tggtctggcc gccggggccg acgaactcca tgccctgcgc cgccaggtag ccggggttgg
     1141 cgcgcagctt gggcagggtt tcgccgcgcg cgatggaggg cgggatgttc cagtacgggt
     1201 tgaactcgat gcggcgcatg tcttcgtcga acagcggggt ccgcgtgccg accgccttgc
     1261 cgacgatcac ccgcatggcg aaggcctggt gcacggcctg gtcctgccgc tgccaggcgt
     1321 acagcatgaa ttcgggcacg ttgacggtga cgaagcgctc ggccgccggc agcggggtca
     1381 ggcgcaggcg ctccagcgcc agcgcgatct ggcgtgcgcg ggcggcgggt ggcacgtcca
     1441 gcgcctccag cgtcgccttg cccagcacgc cgtcggcgct caggccgtgg cgctcctgga
     1501 aggccttgac ggcgtgctgc agcgcgggat cgaacgtgtc cggttcggtg gccggggcgg
     1561 cgtccagatc gcccagcgcg tgcaggcgcg ccgccagcgc cgccagcccg ggccagcgct
     1621 ggccggggcg caagcggtcg ccggacaggg gtggcagcgc ggtgcgccag gccgggtcgt
     1681 ccaccagtgc gcggtactgc gccagggcgg tgcgcagcag cgggtactga ggccagggcg
     1741 gggtggcggc ggccagggcc tcggccggcc ggccgttgcg caccgcgtcg tgcaggcgct
     1801 cggccaggtc gggcgcgggc tgaccgacgc tgtcgtagcg ggcgcccagg gcggcggcgg
     1861 gcacgcggcc gtggcgcagt tcgtgcaggt agcgcgtcag ggtctgcgtc agggccgcgt
     1921 cccaggcgcc ggcctcggtc gccgccagcc gctggccggg gtcgatgctg cgcgcccagc
     1981 gcgcggcgtc gtagtcgtgc ggctgcaggc cgtcgcgctc ggcctgcgtc agccagccga
     2041 tcgcctcgcg cgcggcgggg ccgggctggc ccagcgcgtc cagccacagc ggctgcgcct
     2101 gcgcccaggg cgccagccac agcagggcgg cggcggcgcc gagacgggcg cgcgtcagca
     2161 tcgccaggcc gggcgcggcg ccgggttcac ttctgcacgt cgcccaggca caggtacttc
     2221 atctccaggt attcggccat gccgtggcgc gagccttcgc gccccaggcc ggattgcttg
     2281 acgccgccga agggcacatg ctcggtcgag atcaggccgg tgttgatgcc caccatgccg
     2341 tattccagcc cctcggccac gcgcgtgatg cggccgacgt cgcgggcgta gaagtagctg
     2401 gccaggccga attcggtggc gttggcagcg tcgatggctt cctgctcggt gcggaagcgg
     2461 aacaccggcg ccagcggacc aaaggtttct tcgcgcgcca cccgcatcgc ggcggtggcg
     2521 ccggcgatca cggtgggctc gtagaactgg cccgagaggc gccggccgcc ggtcagcacc
     2581 ttgccgcctt tcctgaccgc gtccttgacg tgctgctcca ccttggccag ggcggccggc
     2641 tcgatcagcg ggccgacggt gacgccgtcc tcgaacccgt tgcccacctt catggccttg
     2701 acgcgggcgg ccagcttctt gacgaaggcg tcgtacacgc cgtcctgcac gtacaggcgg
     2761 ttggcgcaga cgcaggtctg gccggcgttg cggtatttgc tggccagcgc gccttcgacc
     2821 gccgagtcga ggtcggcgtc gtcgaacacg atgaagggcg cgttgccgcc cagctccagc
     2881 gccagcttct tgacggtggg cgcgcattgc gccatcagga tgcggccgac ctcggtcgag
     2941 ccggtgaacg acaggtggcg cacgatgtcg ttggcgcaca gctcgcgacc gatggccggc
     3001 gcgtccagcc cggtgacgac gttgatgacg ccggccggga tgccggcgcg ctgggccagc
     3061 tcggccgcgg ccagcgcggt cagcggggtg aattcggccg gcttgatgac caccgtgcag
     3121 ccggccgcca gcgccggcgc caccttgcgc gtgatcatgg cgagcgggaa gttccacggc
     3181 gtgatggcgg cgcacacgcc gatcggctgc tggatcacgg tcaggcgctt gttgcggtcg
     3241 aacggggtca gggtttcgcc ctgcacgcgc ttggcttctt cggcgaacca ttcgacgaag
     3301 ctggcgccat aggcgacttc gcccttggct tcggccagcg gtttgccctg ttcggcggtc
     3361 atcaggcgcg ccaggtcgtc ctggtgctgc atcagcagct ggaaccaggc catcaggtgg
     3421 gcgtggcgct gcttgccggt cagctcgcgc caggccggcc aggcggcgtt ggcggcctgg
     3481 atggcgctgc gggtttcgcg cgcgcccagg tcggccacgt cggccagctt gcggccggtg
     3541 gccgggtcgc tgacgtcgaa acgcgccttg cccttgaccc atttgccgtt gatcagggcg
     3601 tcggtcttga gcaggc
//
LOCUS       DAOOOG010000221         3587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_31472_length_3587_cov_30.838052, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000221 DAOOOG010000000
VERSION     DAOOOG010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31472_length_3587_cov_30.838052"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..3340
                     /gene="ppc"
                     /locus_tag="PK242_12300"
     CDS             143..3340
                     /gene="ppc"
                     /locus_tag="PK242_12300"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HOZ94842.1"
                     /translation="MRTTRTAPAATPTASPARRPSRSDKELPLIQDIRLLGRLLGDVI
                     REHEGTATYELVERIRTLSVAFRRDADQRAGNTLRRLLNGLSGEQTVSVIRAFTCFSL
                     LANLAEDRHHIRRRAVHERAGSAPEGGIEVALQRLHAAGIDAESIARTLSDSYVAPVL
                     TAHPTEVQRKSILDAEREIAQLLAQRDELRERALCQPGGRDVLSPRQLAANETRLHAR
                     VVLLWQTRLLRYDRLSVADEIENALSYYESTFLREIPRLYADLEQALGAARPVASFLR
                     MGQWIGGDRDGNPNVSAQTLHLALRRQAEAALLHYLDEVHELGRTLSLSSRLTAVSPA
                     LAALAQRSPDTSTHRSDEPYRRALTGIYARLSATLERLAGRGAPRPPAVQLAAYARAD
                     ELLAELRTIEGSLRAHDGQALVADRLQPLMRAVEVFGFHLATVDLRQSSDQHERVVAE
                     LLARAQLAPDYASLDEHARCALLLRLLNDARPLRVVGGQYSAHTQGELAIFEAALALR
                     HSHGHDAIRHYIISHTETVSDLLEVLLLQKEVGLMRGTLGGEATADLIVVPLFETIAD
                     LRHAAPIMRAFYAIPGIAALVRASGAEQDIMLGYSDSNKDGGIFTSNWELYRAEIALV
                     ALFDELNREHAEAPIRLRMFHGRGGTVGRGGGPSYQAIMAQPPGTVRGQIRLTEQGEV
                     IASKYANPEIGRRNLETLVAATLEATLLQPTQAAPDEFLQAAEQLSRASMAAYRALVY
                     DTPGFADYFFTATPIREIAQLNIGSRPASRKASDRIEDLRAIPWGFSWGQCRLTLPGW
                     YGFGAAVQTFMDDPAAPPRARLALLRRMHREWPFFRTLLSNLDMVLAKSDLALGSRYS
                     ELVADRRLRRRVFGAIEAEWQRTNASRKASDRIEDLRAIPWGFSWGQCRLTLPGWYGF
                     GAAVQTFMDDPAAPPRARLALLRRMHREWPFFRTLLSNLDMVLAKSDLALGSRYSELV
                     ADRRLRRRVFGAIEAEWQRTNDALTQITGSRQRLAGNAALARSIRHRFPYIDPLHHLQ
                     VELLRRWRAQPGDERVQTGIHLCINGIAAGLRNTG"
     gene            complement(3395..>3587)
                     /locus_tag="PK242_12305"
     CDS             complement(3395..>3587)
                     /locus_tag="PK242_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOZ94843.1"
                     /translation="EILREARQFNPKEFRIVAAPAVVEMLLDEESQHLAGLSDFIGKP
                     ISLQAESSIGPDQYDIVLL"
BASE COUNT          546 a         1366 c         1180 g          495 t
ORIGIN      
        1 gccaaacgca aggtactgcc ctggctgctc aagtccctgc ccagcctgcc acagccggtg
       61 gcgcaggcgc tgaaaaagaa gatcgcgccc tgacccgcgc gcggcggccc gcggcgccat
      121 cgccgcgtgg acaatcccgc ccatgcgcac cacccgaacc gcccctgccg ccacccccac
      181 cgccagtccc gcccgacgcc ccagccgctc ggacaaggag ctgccgctga tccaggacat
      241 ccgcctgctg gggcgcctgc tgggcgatgt gatccgcgag cacgaaggca ccgccaccta
      301 cgaactggtg gaacgcatcc gcaccctgtc ggtggcgttc cggcgcgacg ccgaccagcg
      361 cgccggcaac accctgcgcc gcctgctcaa tggcctgagc ggtgagcaga cggtgagcgt
      421 gatccgtgcc ttcacctgtt tcagcctgct ggccaacctg gccgaggacc gccaccacat
      481 ccgtcgccgc gccgtgcatg aacgcgccgg cagtgcgccg gaaggtggca tcgaggtcgc
      541 cctgcaaagg ctgcacgccg ccggcatcga cgccgaatcg atcgcccgca cgctgtcgga
      601 cagctacgtc gccccggtgc tcacggccca cccgaccgag gtgcagcgca agagcatcct
      661 cgacgccgag cgcgagatcg cccagttgct ggcccagcgc gacgagttgc gcgaacgcgc
      721 cctgtgccag cccggcggtc gcgacgtgct gtcgccacgc cagcttgccg ccaacgaaac
      781 ccgcctgcac gcccgcgtgg tgctgctgtg gcaaacccgc ctgctgcgct acgaccgcct
      841 gagcgtggcc gacgaaatcg aaaacgccct gtcctattac gagtccacct tcctgcgcga
      901 gattccgcgc ctgtacgccg acctggagca ggcgctcggc gccgcacggc cggtggccag
      961 ctttctgcgc atggggcagt ggatcggtgg tgaccgcgac ggcaacccca acgtcagcgc
     1021 acagacgctg cacctggcgc tgcgccgcca ggccgaagcg gcgctgctgc actacctgga
     1081 cgaagtgcac gagctgggtc gcacgctgtc gctgtcgtcg cggctgaccg cggtatcgcc
     1141 cgccctggcg gcgctggcgc agcgctcgcc ggacaccagc acgcaccgca gcgacgagcc
     1201 ctaccggcgc gcactgaccg gcatctacgc ccgcctgtcg gccacgctgg agcggctggc
     1261 cgggcgcggc gcgccgcgtc cgcccgcggt gcaactggcc gcttatgcgc gggccgacga
     1321 actgctggcc gagctgcgca ccatcgaggg ttcgctgcgc gcccacgatg gccaggccct
     1381 ggtggccgac cggttgcagc cgctgatgcg tgcggtcgag gtgttcggct ttcacctggc
     1441 cacggtggat ctgcgccaga gctccgacca gcacgagcgc gtggtcgccg agctgctggc
     1501 gcgcgcgcag ctggcgcccg actacgccag cctggacgaa cacgcgcgct gcgccctgct
     1561 gctgcgcctg ctgaacgatg cacggccatt gcgggtggtg ggcgggcagt acagcgcgca
     1621 cacccagggc gagctggcca tcttcgaggc cgcgctggcg ctgcgccaca gccacggcca
     1681 cgacgccatc cgccactaca tcatcagcca caccgaaacc gtcagcgacc tgctggaggt
     1741 gctgttgctg cagaaggaag tcggcctgat gcgcggcacc ctggggggcg aggccacggc
     1801 cgacctgatc gtggtgccgc tgttcgaaac catcgccgac ctgcgccatg ccgcccccat
     1861 catgcgcgcc ttctatgcca tccccggcat cgccgcgctg gtgcgtgcca gtggtgccga
     1921 gcaggacatc atgctgggct acagcgacag caacaaggac ggcggcatct tcaccagcaa
     1981 ctgggagctg taccgggccg agatcgccct ggtggcgctg ttcgacgaac tcaatcgcga
     2041 gcacgccgaa gcgccgatcc gcctgcgcat gttccacggc cgcggcggca ccgtgggccg
     2101 cggcggcgga cccagctacc aggccatcat ggcgcagccc cccggcacgg tgcgcgggca
     2161 gattcgactg accgagcagg gcgaggtcat cgcctccaag tacgccaacc ccgaaatcgg
     2221 ccgccgcaac ctggaaaccc tggtcgccgc cacgctggaa gccacgctgc tgcaacccac
     2281 ccaggccgcc cccgacgaat tcctgcaggc ggccgagcag ctctcgcgcg ccagcatggc
     2341 ggcctaccgc gccctggtgt acgacacgcc cggctttgcc gactacttct tcaccgccac
     2401 gccaatccgc gagatcgccc agctcaacat cggctcgcgg ccggccagcc gcaaggcgag
     2461 cgatcgcatc gaggacctgc gcgccatccc ctggggcttc agctggggcc agtgtcgcct
     2521 gaccctgccc ggctggtacg gcttcggcgc cgccgtgcag accttcatgg acgatcccgc
     2581 cgccccgccg cgcgcgcgtc tggcgctgct gcggcgcatg caccgcgagt ggccgttctt
     2641 tcgcaccctg ctgtcgaacc tggacatggt gctggccaag agcgacctgg cgctgggttc
     2701 gcgctacagc gaactggtgg ccgaccgccg gctgcggcgc cgcgtgttcg gcgccatcga
     2761 ggccgaatgg cagcgcacca acgccagccg caaggcgagc gatcgcatcg aggacctgcg
     2821 cgccatcccc tggggcttca gctggggcca gtgtcgcctg accctgcccg gctggtacgg
     2881 cttcggcgcc gccgtgcaga ccttcatgga cgatcccgcc gccccgccgc gcgcgcgtct
     2941 ggcgctgctg cggcgcatgc accgcgagtg gccgttcttt cgcaccctgc tgtcgaacct
     3001 ggacatggtg ctggccaaga gcgacctggc gctgggttcg cgctacagcg aactggtggc
     3061 cgaccgccgg ctgcggcgcc gcgtgttcgg cgccatcgag gccgaatggc agcgcaccaa
     3121 cgacgcgctg acgcagatca ccggcagccg ccagcgcctg gccggcaacg ccgcgctggc
     3181 gcgctcgatc cgccaccgct ttccctacat cgacccgctg catcacctgc aggtcgagct
     3241 gctgcgccgc tggcgcgccc aacccggcga cgagcgcgtg cagaccggca ttcacctgtg
     3301 catcaacggc attgccgccg ggctgcgcaa caccggctga tgccacgacc cggccggtgc
     3361 gggcccggcg cgcaccaggc gcgtgcggcg ccgcctacag caacacgatg tcgtactggt
     3421 cgggcccgat gctggattcg gcctgcagcg aaatcggctt gccgatgaag tcggacaggc
     3481 ctgccagatg ctggctttcc tcgtccagca gcatctcgac cacggccgga gcggcgacga
     3541 tgcggaactc cttggggttg aactggcgcg cctcgcgcag gatttcg
//
LOCUS       DAOOOG010000222         3583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_31525_length_3583_cov_24.040249, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000222 DAOOOG010000000
VERSION     DAOOOG010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31525_length_3583_cov_24.040249"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PK242_12310"
     CDS             complement(<1..424)
                     /locus_tag="PK242_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HOZ94844.1"
                     /translation="METLAPDTALDHAADAAPPEPATQPASSAPSPGSVAALSGLSAL
                     LASGLAACGGGGEATQADPPAAPISAAEAARFLQQAQFSSSDADIAAVRSQGYARWLD
                     AQMQAPSHTSGWDWLVEQGYQQESLRFNGGYADHMMWNQ"
     gene            complement(558..1535)
                     /gene="miaA"
                     /locus_tag="PK242_12315"
     CDS             complement(558..1535)
                     /gene="miaA"
                     /locus_tag="PK242_12315"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOZ94845.1"
                     /translation="MATPSATEAAGLRPIGIAGPTASGKSALALALAERLPVEIISVD
                     SALVYRGLDIGSAKPSLAERAAVPHHLIDIRDPSQPYSAAEFVRDATRLMAEIRARGR
                     TPLLVGGTMLYFKALQQGLDEMPPADPTVRAQLEARARQHGWAALHAELARVDPASAA
                     RLPPGDSQRIQRALEVWQLSGRPLSSFHATNSVAAGAHQVNAWPLFSLEPQDRAWLHR
                     RIAERFDAMLSAGLLDEVAALRARGDLSPQLPALRAVGYRQAWQALEQGWPLALLRDR
                     GSAATRQLAKRQLTWLRGMPWRRPLPADAADALPRAVQLILATARGNEK"
     gene            complement(1513..2763)
                     /locus_tag="PK242_12320"
     CDS             complement(1513..2763)
                     /locus_tag="PK242_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HOZ94846.1"
                     /translation="MSSPASVTGPGRAPAAMSASTVVVLLGLLLGLQPVTTDVYLPAL
                     PALQHELAASMSQVQLTFAGLLLAFGCSQLVWGPLSDRFGRRPVLLWGMGAYLLASLA
                     SALAPSMPLLIAARVAQGAAMGAAVMCARAIVRDLYEPLEGARMMSRGLSGLGVIAVT
                     CAPVGGLLTELIHWRAALAALALFGALTLALVALRFHETIPARNPRALAPGTLWQAWR
                     RIARHPTFVTYSLLATASYAALFTFLSTSPFVLIETLGLSRFGYGVLMAAGSLVYIAG
                     TMACRRLLQRWGIRRSVWAAGWVTFTAGALMAGLAWLGWGRPWYGWWAVFVPQALFLL
                     AHGVHQPVSQSGTVAPFPDMAGAASALNGFIMMAVAFPMGLWLGRMMDGTPRALALGF
                     FGWSTVIALTAWFLVPRHGDPERH"
     gene            complement(2882..>3583)
                     /locus_tag="PK242_12325"
     CDS             complement(2882..>3583)
                     /locus_tag="PK242_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94847.1"
                     /translation="QAAIDFKAPAGHAVAELQALWQAAPDAGAAALPAAPADDDWPLG
                     RAIAQLHGIYILAENRAGLVLVDMHAAHERIVYERLKRQLDGARVASQPLLIPATFAA
                     TAQEVATAEGCAEVLARLGLEITAFSPRTLAVRAVPTALAAGDAVALARSVLAELAQH
                     EASTVVERARHELLATMACHGAVRAHRRLTHEEMNALLRQMEATERSDQCNHGRPTWR
                     QLSLRELDALFLRGR"
BASE COUNT          502 a         1251 c         1383 g          447 t
ORIGIN      
        1 gctggttcca catcatgtgg tcggcgtagc cgccgttgaa gcgcaggctt tcctgttgat
       61 agccttgctc gaccagccag tcccagccgc tggtgtggct gggcgcctgc atctgcgcgt
      121 ccagccagcg cgcgtagccc tgcgaccgca cggcggcgat gtcggcgtcg ctggacgaaa
      181 actgcgcctg ctgcagaaag cgcgccgcct cggcggccga gatcggggcg gcaggcggat
      241 ccgcctgagt ggcctcgccg ccgcccccgc aggcggccag gccgctcgcc agcagcgccg
      301 acaggccgct cagggcggcg acgctgcctg gcgagggcgc cgacgatgcg ggctgggtgg
      361 cgggttcggg cggggccgcg tctgcggcgt ggtcgagggc ggtgtcgggg gcgagcgtct
      421 ccatgcgtgt cgtgttctgg cgctggcccg caagctcctc tgacgtgagt tccccgggct
      481 ggatctcccc ggcgcggatt agaccgcagt tggccgccgt gccggcgctg cggcacgaaa
      541 cgttagttac aaagtgttca tttctcgttg ccccgagcgg tggccaggat cagctgcacc
      601 gccctgggca gggcgtcggc agcgtcggcc ggcagcggcc ggcgccaggg catgccgcgc
      661 agccaggtga gctggcgctt ggcgagctgg cgcgtggcgg cgctgccgcg gtcgcgcagc
      721 agcgccagcg gccagccctg ttccagcgcc tgccaggcct ggcggtaacc gacggcgcgc
      781 agcgcgggca gctggggcga cagatcgccg cgcgcccgca gcgccgccac ctcgtcaagc
      841 aggccggcgc tcagcatggc gtcgaagcgc tcggcgattc gacggtgcag ccaggcgcgg
      901 tcctgcggtt ccagagaaaa aagcggccag gcgtttacct ggtgtgcgcc agcagctacg
      961 ctatttgtag cgtgaaagct ggacagcggc cggccgctga gctgccacac ttcgagcgcg
     1021 cgctggatgc gctggctgtc gccgggcggc aggcgggcgg cgctcgccgg gtcgacacgc
     1081 gccagctcgg cgtgcagggc ggcccagccg tgctggcgcg cgcgcgcctc cagctgggcg
     1141 cgcacggtgg ggtcggccgg cggcatttcg tccaggccct gttgcagcgc cttgaaatac
     1201 agcatggtgc cgccgaccag cagcggcgtg cgtccgcgcg cacggatctc ggccatcagc
     1261 cgggtggcgt cgcgcacgaa ctcggcggcg ctgtagggct ggctggggtc gcggatgtcg
     1321 atcaggtggt gcggcacggc ggcgcgttcg gccaggctgg gcttggcgct gccgatgtcc
     1381 agcccgcggt agaccagggc cgagtcgacg ctgatgattt ccaccggcaa ccgctcggcc
     1441 agcgccaacg ccagcgcgct cttgccgctg gcggtggggc cggcgatgcc gatcggccgc
     1501 aggccggcag cttcagtggc gctcggggtc gccatgcctt ggcaccagaa accaggcggt
     1561 gagggcgatc accgtgctcc agccgaagaa gcccagcgcc agcgcgcgcg gcgtgccgtc
     1621 catcatgcgg cccagccaca ggcccatggg aaaggccacc gccatcatga tgaagccgtt
     1681 gagcgccgag gccgcgcccg ccatgtcggg aaacggcgcc accgtgccgc tctggctgac
     1741 cggctgatgc acgccgtggg ccagcaggaa cagcgcctgc ggcacgaaca ccgcccacca
     1801 gccgtaccag ggccggcccc agcccagcca ggccaggccc gccatcagcg cgccggcggt
     1861 gaaggtgacc cagccggccg cccacacgct gcgccggatg ccccagcgct gcagcaggcg
     1921 gcggcaggcc atggtgccgg cgatgtacac cagcgagccg gccgccatca gcacgccgta
     1981 gccgaagcgg ctcaggccca gcgtctcgat cagcacgaag ggcgaggtgg aaagaaaggt
     2041 gaacagcgcc gcgtaggagg ccgtggccag cagcgaatag gtgacgaagg tcgggtggcg
     2101 ggcaatgcgc cgccaggcct gccacagcgt gcccggcgcc agcgcccggg ggttgcgggc
     2161 gggaatggtt tcgtgaaagc gcagcgccac cagcgccagc gtcagggccc cgaacagcgc
     2221 cagcgccgcc agcgccgcgc gccagtggat cagctcggtc agcagcccgc ccaccggcgc
     2281 gcaggtgacg gcgatcacgc ccaggcccga caggccgcgc gacatcatgc gcgcgccttc
     2341 cagcggctcg tacaggtcgc gcacgatggc gcgtgcgcac atcaccgccg cccccatggc
     2401 cgcgccctgg gccacgcggg cggcgatcag cagcggcatg ctgggtgcca gcgcgctggc
     2461 cagcgaagcc agcagatagg cgcccatgcc ccacagcagc accggccggc ggccaaagcg
     2521 gtccgacagc ggcccccaca ccagctgcga gcagccaaag gccagcagca ggccggcgaa
     2581 ggtcagctgc acctgcgaca ttgaggccgc cagctcgtgc tgcagggccg gcagcgccgg
     2641 caggtagacg tcggtggtga ccggctgcag gcccagcagc aggcccagca ggacgacgac
     2701 ggtggaggcg ctcatggccg ccggggcgcg cccggggccg gtgacggaag cgggggaaga
     2761 cacgcgggcg agggtagcag atgacggatg gcggatgacc gcgcggcgtg tcgatcgcca
     2821 gggcgcggct caggtttgcg cggccggggt caggggtcga gcgcctgggc gccgccgggc
     2881 ctcagcggcc gcgcaggaac agggcgtcga gctcgcgcag gctgagctgg cgccaggtcg
     2941 ggcggccgtg gttgcactgg tcgctgcgct cggtggcttc catctggcgc agcagggcgt
     3001 tcatttcttc gtgcgtcagg cgccggtggg cgcgcaccgc gccgtggcag gccatggtgg
     3061 ccagcagctc gtggcgggcg cgttcgacca cggtgctggc ctcgtgttgc gccagctcgg
     3121 ccagcacgct gcgcgccagc gccacggcgt cgccggccgc cagcgcggtg ggcacggcgc
     3181 gcaccgccag cgtgcggggc gagaacgcgg tgatctccag gcccaggcgg gccagcacct
     3241 cggcgcagcc ttcggcggtg gccacttcct gcgcggtggc ggcaaaggtg gccgggatca
     3301 gcagcggctg gctggccacg cgggcgccgt cgagctggcg cttgagccgc tcgtagacga
     3361 tgcgttcgtg cgccgcatgc atgtccacca gcaccaggcc ggcgcggttt tcggccagga
     3421 tgtagatgcc gtgcagctgc gcgatggcgc ggcccagcgg ccagtcgtcg tcggccgggg
     3481 cggcgggcag ggcagccgcg ccggcgtcgg gtgcggcctg ccacagggcc tgcagctcgg
     3541 ccacggcgtg gcccgcaggt gctttgaaat cgatagctgc ttg
//
LOCUS       DAOOOG010000223         3487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_32666_length_3487_cov_30.313228, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000223 DAOOOG010000000
VERSION     DAOOOG010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32666_length_3487_cov_30.313228"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..939)
                     /locus_tag="PK242_12330"
     CDS             complement(73..939)
                     /locus_tag="PK242_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ94848.1"
                     /translation="MNQELVRLIERAEQLISRIESVLPQPLSAPDWTQAVAWRYRQRA
                     SGHGALEPVRHVGAITLADLKEIDVQKDKIERNTRQFVEGRPANNVLLTGARGTGKSS
                     LIKACLNAFAPQGLRLIEVDKQDLTDLPDIVEVVAGRPEKFIVYCDDLSFDEGEPGYK
                     ALKSILDGSVAASTPNVLVYATSNRRHLLPEYMKDNLSYTHTDDGEVHPGEVVEEKIS
                     LSERFGLWVSFYPFSQDEYLAIAAQWLGALGATPAQIAAARPEALVWALERGSRSGRV
                     AYQFARDYMGQH"
     gene            complement(1038..2267)
                     /gene="argJ"
                     /locus_tag="PK242_12335"
     CDS             complement(1038..2267)
                     /gene="argJ"
                     /locus_tag="PK242_12335"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOZ94849.1"
                     /translation="MPVHLNAPDPTQLHPVAGVRIGVAEAGIRKAGRKDLTVMLLDEG
                     ASIGGVFTQNRFCAAPVQICRQHLQAGAAVRAIVVNTGNANAGTGDDGLARARRSCQA
                     LAGLLQLRPEQVLPFSTGVIMEPLPIERIEAGLPAAIADARPAHWARAAEGIMTTDTV
                     PKAASARARVGGVDITVTGIAKGAGMIRPNMATMLGFVATDAAIDPALMTALARELAD
                     GSFNRITIDGDTSTNDAFLVIATHRAGNAPVTALDSADGQALKAALLSVARQLAQAIV
                     RDGEGATKFITVRVEGGQTAGECRQVAYAIAHSPLVKTAFFASDPNLGRILAAVGYAG
                     IADLDQTRIDLYLDEVHVATRGGRHPDYREEDGQRVMKQQEITVRVVLGRGGAADTVW
                     TCDLSHDYVSINADYRS"
     gene            complement(2406..>3487)
                     /locus_tag="PK242_12340"
     CDS             complement(2406..>3487)
                     /locus_tag="PK242_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOZ94850.1"
                     /translation="ATERHESRRIDNQLRGRSGRQGDPGSSRFYLSLDDALMRIFAGD
                     RVRAIMDKLKMPEGEAIEAGIVTRSIESAQRKVEARNFDIRKQLLEYDDVSNDQRKVI
                     YQQRNEILDASDLSAQIASLREGCFTDLVRQFVPAESVEEQWDIAGLEKALADDWHIE
                     LPLRQQIESSSAISDEDILERVLKAADEAYAAKVAQVGAENFGPFERAVLLQAIDTHW
                     REHLSALDYLRQGIHLRGYAQKQPKQEYKREAFELFGQLLDVIKNEVTRVLMTVRIES
                     PEQAGQSAAAMEQRAEHISNVTYTAPTETGEAQTVTEAQARGAAAAAGGEVAAAAAAV
                     PRVGRNDPCPCGSGKKYKQCHGALT"
BASE COUNT          459 a         1142 c         1272 g          614 t
ORIGIN      
        1 cccaagtggc gcaaggtcag cgttcatgcg gcattcccaa gggttgcaag cggtccactg
       61 cggcttccag gctcaatgct ggcccatgta gtcgcgcgcg aactggtacg ccacccggcc
      121 cgagcgcgag ccacgttcga gggcccacac cagcgcctcg gggcgcgcgg cggcgatctg
      181 cgccggcgtg gcgcccagcg cgcccagcca ctgggccgcg atggccaggt actcgtcctg
      241 gctgaacgga tagaagctga cccacaggcc gaagcgctcg gacagcgaga tcttctcctc
      301 caccacctcg cccggatgca cttcgccgtc gtcggtgtgg gtgtagctga ggttgtcctt
      361 catgtactcg ggcagcaggt ggcggcggtt gctggtggcg tagaccagca cgttgggggt
      421 gctggcggcc acgctgccgt ccagaatgct cttgagcgcc ttgtagcccg gctcgccctc
      481 gtcgaagctg agatcgtcgc aatagacgat gaacttctca ggccggccgg ccaccacctc
      541 gacgatgtcg ggcaggtcgg tcaggtcctg cttgtcaacc tcgatcaggc gcagcccctg
      601 cggcgcaaag gcgttcaggc aggccttgat gagcgaggac ttgccggtgc cgcgcgcccc
      661 ggtcagcaac acgttgttgg ccggccgccc ttcgacgaac tggcgtgtgt tgcgctcgat
      721 cttgtccttc tggacgtcga tttccttgag gtcggccaac gtgatggcgc cgacgtggcg
      781 caccggctcc agcgcgccgt ggccgctggc gcgctggcgg tagcgccagg ccacggcctg
      841 cgtccagtcg ggcgccgaca gcggttgcgg cagcaccgat tcgatgcggg agatgagttg
      901 ttcggcccgt tcgatcagcc gcacgagttc ctgattcatt tcggcatcca gcgcttggtg
      961 gatcagcgcc aatagctatc cattcaatag taacgacggc tttcgccgcc ggcccgtcgg
     1021 gccgaccagc cgacggctca cgagcggtag tcggcgttga tgctgacgta gtcgtggctg
     1081 aggtcgcagg tccacacggt gtcggcggcg ccaccgcggc cgagcaccac gcgcacggtg
     1141 atttcctgct gcttcatcac gcgctggccg tcttcctcgc ggtagtcggg gtgacggccg
     1201 ccgcgcgtgg ccacgtgcac ctcgtcgagg tagagatcga tgcgcgtctg gtcgaggtcg
     1261 gcgatgccgg cgtagccgac ggcggccagg atgcggccaa ggttggggtc gctggcgaag
     1321 aacgccgtct tgaccagcgg cgaatgggcg atggcgtagg ccacctggcg gcactcgccg
     1381 gccgtctggc cgccttcgac gcggacggtg atgaacttgg tggcaccctc gccgtcgcgc
     1441 acgatggcct gggccagctg gcgcgccacc gacagcagcg ccgccttcag cgcctggccg
     1501 tcggccgaat cgagcgcggt gacgggcgcg ttgccggcgc ggtgggtggc gatgaccagg
     1561 aaggcatcgt tggtcgaggt gtcgccgtcg atggtgatgc ggttgaacga gccgtcggcc
     1621 agttcgcgcg ccagcgcggt catcagcgcc gggtcgatgg cggcgtcggt ggccacgaag
     1681 cccagcatgg tggccatgtt ggggcggatc atgccggcac ccttggcgat gccggtgacg
     1741 gtgatgtcga ccccgcccac gcgggcgcgg gcgctggcgg ccttgggcac ggtgtcggtg
     1801 gtcatgatgc cttcggcggc gcgcgcccag tgggcgggcc gcgcgtcggc gatggcggcc
     1861 ggcaggccgg cttcgatgcg ctcgatcggc agcggctcca tgatgacgcc ggtggaaaac
     1921 ggcagcacct gctcgggccg cagctgcagc aacccggcca gcgcctggca gctgcggcgc
     1981 gcgcgcgcca ggccgtcgtc accggtgccg gcgttggcgt tgccggtgtt gaccacgatg
     2041 gcgcgcaccg cggcgccggc ctgcaggtgc tggcggcaga tctgcaccgg cgcggcgcag
     2101 aagcggttct gcgtgaacac gccgccgatg ctggcgcctt cgtcgagcag catcacggtc
     2161 aggtccttgc ggccggcctt gcggatgccg gcttcggcca cgccgatgcg cacgccggcg
     2221 accgggtgca gttgcgtggg atcgggagcg ttcaggtgga ccggcatgac aggttccttc
     2281 gatcggcaga ttgtgaatcc cataggccgc tagcgcttgt cggtcaagcg ccagcagcta
     2341 tcaaataaat agcttctggc ggtgttccgc aggtgcccga accggggcgg cgccggcagc
     2401 agcggtcaag tgagcgcgcc gtggcactgc ttgtatttct tgccgctgcc gcacgggcag
     2461 gggtcgttgc ggccgacgcg tggcaccgcc gccgccgccg ccgccacctc gccaccggcc
     2521 gcggcggcgg cgccgcgcgc ctgcgcttcg gtcacggtct gggcctcgcc ggtttcggtg
     2581 ggcgcggtgt aggtgacgtt gctgatgtgc tcggcgcgct gctccatggc ggcggcgctc
     2641 tggccggcct gttcggggct ttcgatgcgc accgtcatca gcacccgcgt gacctcgttc
     2701 ttgatcacgt ccagcagctg gccgaacagc tcgaaggctt cgcgcttgta ttcctgcttg
     2761 ggctgcttct gggcatagcc gcgcaggtgg atgccctggc gcaggtagtc gagcgccgac
     2821 aggtgttcgc gccagtgggt gtcgatggcc tgcagcagca cggcgcgctc gaaggggccg
     2881 aagttctcgg cgccgacctg cgccaccttg gcggcgtagg cttcgtcggc ggccttgagc
     2941 acgcgctcga ggatgtcctc gtcgctgatg gcggaggacg actcgatctg ctggcgcagc
     3001 ggcagctcga tgtgccagtc gtcggccagc gccttttcca ggccggcgat gtcccactgc
     3061 tcctcgaccg attcggccgg cacgaactgg cgcaccaggt cggtgaagca gccttcgcgc
     3121 aggctggcga tctgggccga caggtcgctg gcgtcgagga tttcgttgcg ctgctggtag
     3181 atcaccttgc gctggtcgtt ggagacgtcg tcgtactcca gcagctgctt gcggatgtcg
     3241 aagttgcgcg cctcgacctt gcgctgcgcg ctttcgatgc tgcgggtgac gatgccggcc
     3301 tcgatggcct cgccctcggg catcttcagc ttgtccatga tggcgcgcac gcggtcgccg
     3361 gcgaagatgc gcatcagcgc gtcgtccagg ctgaggtaga agcgcgagga gcccgggtcg
     3421 ccctggcggc ccgagcggcc gcgcagctgg ttgtcgatgc ggcggctttc gtgccgctcg
     3481 gtggcga
//
LOCUS       DAOOOG010000224         3474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_32827_length_3474_cov_27.629716, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000224 DAOOOG010000000
VERSION     DAOOOG010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32827_length_3474_cov_27.629716"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="PK242_12345"
     CDS             <1..107
                     /locus_tag="PK242_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94851.1"
                     /translation="VKALYELQRIQGRYALVTMCIGGGQGIAAIFERM"
     gene            226..645
                     /locus_tag="PK242_12350"
     CDS             226..645
                     /locus_tag="PK242_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94852.1"
                     /translation="MRLDPFRRVDDTPFSARRTDIERARGQPLRVEPNAVGLTALDYG
                     DVVFRLQDSSGRLEEVTRRAPVLYLLRGDSVVDVPFAALAGYVRTHDARAFERGGFLV
                     SPRLGLAFVPTEPDWVTALAAHCVDAWRRLGNDAAQE"
     gene            complement(656..1039)
                     /locus_tag="PK242_12355"
     CDS             complement(656..1039)
                     /locus_tag="PK242_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94853.1"
                     /translation="MTTLTLTTLYTGPDGRARFRDDRLALPDGTPAARLSALQASGGC
                     QFRHSPVGFRSDFHCTTTPQWLVVLQGRMEIGLQDGSTRVFGPGQCFYSNDTLPPGAA
                     FDPSVHGHCSRQLGDEPLVTLFVRA"
     gene            1163..1996
                     /locus_tag="PK242_12360"
     CDS             1163..1996
                     /locus_tag="PK242_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94854.1"
                     /translation="MKALATLLGALALSGGVLLASPAQAAGQWFDVHVIDRDSGSRLP
                     LYRHDGQWWVAGRPGARYAIELRNTSGTRLLGVVSVDGVNVISGETADWQQSGYVLAP
                     HQRAQITGWRKSEAEVAAFHFTALPHAYATRTGRPEHVGVIGVAVFRERTPAPPPPEW
                     SEHRRAPAAGEAPPSPAAPAAAAADSESGAASRREAQAAAPRLGTGHGAREPAWVTRT
                     RFERRSSQPDEVIAIRYDSRENLLAMGVIPQHPRQAQAPRPFPDAPPGPGYTPDPPAW
                     R"
     gene            2048..2647
                     /locus_tag="PK242_12365"
     CDS             2048..2647
                     /locus_tag="PK242_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ94855.1"
                     /translation="MPAPVASADEPLMRAYAAGDASAFETLYDRHQLRLWRYLLRSVG
                     DAATADELAQDVWLRVAREAPRYTPSAAAPGRPPARFTTWLFTLAHHRVVDHLRRQRP
                     TLSLDEPLHDELTLADTLAAPSGFGPLRQIETRQQAVQLLAALDALPPEQRTAFLLQA
                     EGEMSVAEIASTTGVGLETARSRLRYARAALRRKLEHLA"
     gene            2644..>3474
                     /locus_tag="PK242_12370"
     CDS             2644..>3474
                     /locus_tag="PK242_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94856.1"
                     /translation="MNAPPALPRPTDDDLVERYHEAAALEDRGPAPELRAAVLAQARM
                     LAWRHPEAADDGPSNATESIAGGDDPASAAAVFDAEAGSPRRPRGAANDRRWLISTAA
                     SVAVLGLAGLLALQFERSPQGLREAAPQAGPTALVDAAPPPAPPATAAAPLAPTPAAA
                     TAAPPAAAPKPETIQPAPAPAAPHPPETTPPATPHLPPPAADAATPATAARAPDPRAA
                     RPKAEPRAREPAARPDLRRPAADARPDRPPPTAPARAEHPEAEAGAPAPRPPQASEAP
                     S"
BASE COUNT          470 a         1404 c         1201 g          399 t
ORIGIN      
        1 ccgtcaaggc gctgtatgag ctgcagcgca tccagggccg ctacgcgctg gtgaccatgt
       61 gcatcggcgg cggtcagggc attgccgcga tcttcgagcg catgtgatcg cacccgcccg
      121 gttgctgcaa gttttgcagc tgcctgcgcc cgctggacaa gcgccagcgg gcttttttat
      181 gggtcaaggt ggcgccctgg ccgggcgccg gagcaggccc gcggcatgag gctcgatccc
      241 ttccggcggg tggacgacac cccgttttcc gctcgccgca ccgacatcga gcgcgcgcgc
      301 ggccagccgc tgcgcgtcga acccaacgcc gtcggcctga cggcgctgga ctatggcgat
      361 gtggtgttcc gcctgcagga cagcagcggc cggctggagg aggtgacgcg ccgcgcgccg
      421 gtgctgtacc tgctccgcgg cgacagcgtg gtcgatgtgc cctttgccgc cctggccggc
      481 tacgtgcgca cgcacgacgc gcgggcgttc gagcgtggcg gctttctggt cagcccgcgc
      541 cttggcctgg ccttcgtgcc caccgaaccc gactgggtga ccgcactggc ggcgcactgc
      601 gtcgacgcct ggcgccggct tggcaacgat gccgcgcagg agtgatgagc cgccgtcagg
      661 cgcgcacgaa caatgtgacc agcggctcgt cgcccagctg acggctgcag tggccgtgca
      721 cggacgggtc gaacgcagcg cccggcggca gcgtgtcgtt ggagtagaag cactgccccg
      781 gcccgaacac gcgcgtgctg ccgtcctgca gcccgatttc catgcgcccc tgcagcacca
      841 ccagccactg cggcgtggtg gtgcagtgga agtcgctgcg aaaacccacc gggctgtgcc
      901 ggaactggca gccgcccgag gcctgcagcg ccgacaggcg ggccgcgggc gtgccatcgg
      961 gcagcgccag ccgatcgtca cgaaagcgcg cccggccatc ggggccggtg tagagcgtgg
     1021 tcagggtcag cgtggtcatg gccaggcagt gtagagcggc gcgtgcgcac acatttgctc
     1081 acgcttgcat ggcgccggcg caaccccgtc gggctcggac cgacgtttca gtgcactcca
     1141 tcttctcaac ctggagttcc gcatgaaagc tcttgccacc ctgctgggtg ccctcgccct
     1201 gagcggcggc gtgctgctgg ccagcccggc gcaggccgcc ggccagtggt tcgacgtcca
     1261 cgtgatcgac cgcgacagcg gcagccggct gccgctgtac cgccatgacg gccagtggtg
     1321 ggtcgccggg cgccccggcg cgcgctacgc catcgaactg cgcaacacca gcggcacgcg
     1381 cctgctgggc gtggtgagcg tggacggcgt caacgtcatc agcggcgaaa ccgccgactg
     1441 gcagcagagc ggctacgtgc tggcgccgca ccagcgggca cagatcaccg gctggcgcaa
     1501 gagcgaggcc gaggtggcgg cgttccactt caccgccctg ccgcacgcct acgccacccg
     1561 caccggccgg cccgagcacg tgggagtgat cggcgtggcg gtgtttcgcg aacgcacgcc
     1621 cgcaccgccc ccgcccgagt ggtcggagca ccgccgcgcg ccggcggccg gcgaagcgcc
     1681 ccccagcccg gcggcgccgg cagcggccgc tgccgattcc gaatccggcg ccgccagccg
     1741 gcgcgaagcc caggccgccg caccgcgcct gggcaccggc catggcgcac gcgagccggc
     1801 ctgggtcacc cgcacccggt tcgagcgccg cagcagccag cctgacgagg tgatcgccat
     1861 ccgctacgac agccgcgaaa acctgctggc catgggcgtg atcccgcagc acccgcgcca
     1921 ggcgcaggca ccgcggccgt ttcccgacgc gccgcccggc ccgggctaca cgcccgaccc
     1981 gccggcctgg cgctgaacgc tccccggcgc tggcgcctgg cgcgcgccgc tggccataat
     2041 ccagcccatg cccgcgccag tggccagcgc cgacgaaccg ctcatgcgtg cctacgccgc
     2101 aggcgacgcc agcgccttcg agaccctgta tgaccggcac cagctgcgcc tctggcgcta
     2161 cctcctgcgc agcgtgggcg acgccgccac ggccgacgag ctggcgcagg acgtgtggct
     2221 gcgcgtggcc cgcgaagcgc cccgctacac ccccagcgcc gccgccccgg gccgcccgcc
     2281 ggcgcgcttc accacctggc tgttcaccct ggcccaccat cgggtggtcg atcacctgcg
     2341 gcgccagcgg ccgaccctca gcctggacga gccgctgcac gacgagctca cgctggccga
     2401 cacgctggca gcgccctcgg gcttcggccc gctgcgccag atcgaaaccc gccagcaggc
     2461 cgtgcagctg ctggcggcgc tggacgcgct gccgcccgag cagcgcaccg ccttcctgct
     2521 gcaggccgag ggcgaaatga gcgtggccga aatcgccagc accaccggcg tcggcctgga
     2581 aaccgccagg agccgcctgc gctacgcccg cgccgcgctg cgccgcaagc tggagcacct
     2641 cgcatgaacg cgccgcccgc cctgccgcgc ccgaccgacg acgatctggt cgagcgctac
     2701 cacgaagccg ccgcgctcga agaccgcggc cccgcgcccg agctgcgcgc ggccgtgctg
     2761 gcccaggccc ggatgctggc ctggcgccat cccgaagcgg ccgacgacgg gccgtcgaac
     2821 gctaccgaaa gcatagctgg tggcgacgat ccagcaagcg ctgcagccgt ttttgatgct
     2881 gaagccggca gtccccgccg gccgcgcggc gcggccaacg accggcgctg gctgatctcc
     2941 accgctgcca gcgtggcggt gctgggtctg gccggcctgc tggccctgca gttcgagcgc
     3001 agtccgcagg gcctgcgcga agcggccccg caggccggcc cgacggcgct ggtggacgcg
     3061 gcgccgccgc ccgccccgcc cgccacagcg gcggctccgc tggcccccac gccggccgca
     3121 gcgacggccg cgcccccggc cgcagcgccg aagcccgaaa ccatccagcc cgcgcctgcg
     3181 cccgcagcgc cccatcctcc cgagaccacg ccgccggcca cgccgcacct gcccccgccc
     3241 gccgccgacg ccgccacgcc agccaccgcc gcgcgagcgc ccgatccccg cgcggccagg
     3301 ccgaaagccg agccgcgcgc ccgcgaaccc gcggctcgcc cggacctgcg tcggcccgcc
     3361 gccgatgcgc gccccgaccg accgccgccc accgcacccg cgcgcgccga acaccccgag
     3421 gccgaggccg gcgcacccgc cccccgcccg ccgcaggcca gcgaggcgcc gtcg
//
LOCUS       DAOOOG010000225         3460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_32996_length_3460_cov_24.858150, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000225 DAOOOG010000000
VERSION     DAOOOG010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32996_length_3460_cov_24.858150"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PK242_12375"
     CDS             complement(<1..271)
                     /locus_tag="PK242_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HOZ94857.1"
                     /translation="MTLTPPPTHPPGHDHRTPEAIYGFDARGVAKRFRHAAIFGALDS
                     LHPGEVMRFVNDHDPLPLLGQMQARYGAAVRVEYRQRAPEGVVIDF"
     gene            complement(304..627)
                     /locus_tag="PK242_12380"
     CDS             complement(304..627)
                     /locus_tag="PK242_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOZ94858.1"
                     /translation="MTFVVTEACIACKYTDCVDVCPTDAFREGPNFLVIEPDDCIDCA
                     VCVPECPVDAIYADEDVPKAQLDFIALNAELARGWKTISRKKPALPDADAWAQVGRKR
                     EQLQR"
     gene            771..1721
                     /gene="cbiB"
                     /locus_tag="PK242_12385"
     CDS             771..1721
                     /gene="cbiB"
                     /locus_tag="PK242_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOZ94859.1"
                     /translation="MPDAVLLACAPALALAVDRLLGEPPARAHPVVWMGRWLGWAGAR
                     LAPAAGAPAQRSDAGVFWLAALSWCAAAATIFVVSALFQHWVLTQPAWLAALALGLFL
                     KPLLAWAMLHDEVLAVEQALAESPQAGRARLARLVSRDVAALAPHQVREAAIESLAEN
                     LNDSLVAPLFWFVLLGLPGAALFRFANTADAMWGYRGERGGRDWTWAGKWAARADDAL
                     AWLPARITALLIGALGRLRGWHRLPAEARRTPSPNGGWPMAAMALALGVRLGKPGVYT
                     LHAAGRVVQPSDTLRACAIGSHGALAATLLACVALVVAVA"
     gene            1931..2980
                     /locus_tag="PK242_12390"
     CDS             1931..2980
                     /locus_tag="PK242_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ94860.1"
                     /translation="MQVPAPGGATGLDSPLHGGPDAAGVPLHDLSTNANACGPWPAAV
                     QALAALDARHYPDPAYTALVRQLADWHGVAPARIVLAASASEFIQRISVAVRLQAGRA
                     AGVWLPPHAYGDYARAAQAADLARCADPAAAALLWACEPSSPLGQAEPGLAARVAALR
                     PGQTLVLDQAYEPLRLSGAPSLDAAALQRVWRLITPNKALGLTGVRAAYAIAPPHADA
                     ALLARLRALAPSWPLGAHGVALLQGWASAPAHDWLAACRDTLRGWKLRQLEWLRRLGW
                     QIEPGDANFFVAACADPASAEGRLDMQTWLCGLRRHGIKLRDCASFGLPGRVRLAVAP
                     PPVQDALLAALEARR"
     gene            2977..>3460
                     /locus_tag="PK242_12395"
     CDS             2977..>3460
                     /locus_tag="PK242_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94861.1"
                     /translation="MTAVCVMVLGTSSGAGKSWLATALCRHYANQGLRVAPFKAQNMS
                     NNARVVAGGPSPQPTPQRGEGASPGALMEAGPSPQPFPEGWEGAGTAGLHPLPRAGEG
                     RGEGAPSPAGGGLGWGPAPAHAGGEIGSAQYFQALAARAVPDVRMNPLLLKPEADTRS
                     Q"
BASE COUNT          427 a         1177 c         1341 g          515 t
ORIGIN      
        1 tgaagtcgat caccacgccc tcgggcgcgc gctggcggta ttcgacgcgc accgcggcgc
       61 cgtagcgcgc ctgcatctgg cccagcaggg gcagcgggtc gtggtcgttg acgaagcgca
      121 tcacctcgcc cggatgcagg ctgtcgaggg cgccgaagat ggcggcgtgg cgaaagcgct
      181 tggccacgcc gcgggcgtcg aagccgtaga tggcttcggg cgtgcggtgg tcgtggccgg
      241 gtgggtgggt cggcggcggg gtcagggtca tggtggcaag gctccggggg gcgggggtgg
      301 ggttcagcgt tgcagttgct cgcgcttgcg gcccacctgc gcccaggcgt cggcatcggg
      361 cagggcgggc ttcttgcgcg agatggtctt ccagccacgc gccagttcgg cgttgagggc
      421 gatgaagtcg agctgcgcct tgggcacgtc ctcgtcggca tagatggcgt cgaccgggca
      481 ctcgggcacg cagacggcgc agtcgatgca gtcgtcgggc tcgatgacca gaaaattcgg
      541 cccttcgcgg aaggcgtcgg tcgggcatac gtccacgcag tcggtgtact tgcaggcgat
      601 gcaggcttcg gtgacgacaa aggtcatgat gtgccacccg tggcgaaaca ttcaaagatg
      661 cacaaagaat acctttttaa gggatgccct gccagcaacg cgggcaaacg cgcccgccgc
      721 gcggcgccgg ccggggtggc ggcgtgatcg ggctgtctgc cgccgtcgcc atgcccgacg
      781 ccgtgctgct ggcctgcgcc ccggcactgg cgctggccgt cgaccgcctg ctgggcgagc
      841 cgccggcgcg cgcgcacccg gtggtgtgga tggggcgctg gctgggctgg gccggtgcgc
      901 ggctggcgcc cgccgcgggg gcgccggcac agcgttcgga tgccggggtg ttttggcttg
      961 cggcgctgtc ctggtgcgcg gcggcagcta cgatttttgt agtcagcgcg ctgtttcagc
     1021 actgggtgct gacgcagccg gcctggctgg cggcgctggc gttggggctg tttctgaagc
     1081 cgctgctggc ctgggccatg ctgcacgacg aggtgctggc ggtggagcag gcgctggccg
     1141 agtcgcccca ggccgggcgc gcacggctgg cgcggctggt cagccgcgac gtggcggcgc
     1201 tggcgccgca ccaggtgcgc gaggccgcca ttgaaagcct ggccgaaaac ctgaacgatt
     1261 cgttggtggc gcctctgttc tggttcgtgc tgctgggcct gccgggggcc gcgctgtttc
     1321 gctttgccaa caccgccgac gccatgtggg gctaccgggg tgaacggggc ggacgcgact
     1381 ggacctgggc tggcaagtgg gccgcgcgcg ccgacgacgc gctggcctgg ctgccggcac
     1441 gcatcacggc gctgctgatc ggcgcgctgg ggcggctgcg cggctggcac cggctgccgg
     1501 ccgaggcgcg ccgcacgccg tcgcccaacg gcggctggcc gatggcggcc atggcgctgg
     1561 cgctgggcgt gcgcctgggc aagccgggcg tgtacacgct gcacgccgcc ggccgggtgg
     1621 tgcagccgtc tgataccctg cgcgcctgcg ccatcggctc gcacggcgcg ctggcggcca
     1681 cgctgctggc ttgcgtggcg ctggtggtgg cggttgcttg aggctgaaca gccggaacat
     1741 ccctacgcag aggacgcaga gattgcgcag agggcgccaa gaattcagaa gaagctttct
     1801 tggtttcttc tgcgtcccct gcgcattctt ggcgtcctct gcgtatggat gttgatgggc
     1861 ctccaacgcc cgcccaacct gcgccaacag ctcctgatat cgtagcaatc aagcatccat
     1921 gtcctgcacc atgcaagtgc ccgccccggg tggcgcgacc ggcctggaca gtccgctgca
     1981 cggcgggccg gacgcggcgg gcgtgcccct gcacgacctg tcgaccaacg ccaacgcctg
     2041 cggcccctgg ccggcggccg tgcaggcgct ggcggcgctg gatgcgcgcc actaccccga
     2101 cccggcctac accgcgctgg tgcggcaact ggccgactgg cacggcgtgg cgccggcgcg
     2161 catcgtgctg gcggccagcg ccagcgaatt catccagcgc atcagcgtgg cggtgcggct
     2221 gcaggcgggc agggcggccg gcgtctggct gccgccacac gcctatggcg actacgcccg
     2281 cgccgcgcag gccgccgatt tggcgcgctg tgccgacccg gccgccgccg cgctgctgtg
     2341 ggcctgcgag ccgtccagcc cgctgggcca ggccgagccg gggctggcgg cgcgggtggc
     2401 ggcgctgcgg ccgggccaga ccctggtgct cgatcaggcc tacgagccgc tgcgcctgtc
     2461 gggggcgccc agcctggacg cggcggcgct gcagcgcgtg tggcgcctga ttacgcccaa
     2521 caaggcgctg ggcctgaccg gtgtgcgcgc ggcctatgcc atcgcgccgc cgcacgccga
     2581 cgccgcgctg ctggcccgcc tgcgcgccct ggcgccgtcg tggccgctgg gcgcgcacgg
     2641 cgtggcgctg ctgcagggct gggccagcgc gccggcacac gactggctgg ccgcatgccg
     2701 cgacacgctg cgcggctgga agctgcgcca gctcgaatgg ctgcgccgcc tgggctggca
     2761 gatcgagccc ggcgatgcta atttttttgt agctgcttgc gcagatccgg caagcgcaga
     2821 aggtcgattg gatatgcaaa catggctttg cggcctgcgc cgccacggca tcaagctgcg
     2881 cgactgcgcc agctttggcc tgcccggccg ggtgcgcctg gcggtggcgc cgccgccggt
     2941 gcaggacgcg ctgctggccg ccctggaggc gcggcgatga cggcggtctg cgtgatggtg
     3001 ctgggcacca gcagcggcgc cggcaagagc tggctggcca ccgccctgtg tcgccactac
     3061 gccaaccagg gcctgcgcgt ggcgccgttc aaggcgcaga acatgagcaa caacgccagg
     3121 gtggtggcgg gcggcccctc accccagccc actccccaga ggggcgaggg agcaagcccc
     3181 ggtgccttga tggaggcggg accctcaccc caaccctttc ctgaggggtg ggagggagcc
     3241 ggaacggcgg ggctgcaccc tctcccgcgc gcgggagagg gccggggtga gggcgctccc
     3301 tcccccgctg ggggagggtt ggggtggggg ccggcgccag cccatgccgg cggcgagatc
     3361 ggcagcgccc agtacttcca ggccctggcc gcgcgcgccg tgccggatgt gcgcatgaac
     3421 ccgctgctgc tcaagcccga ggccgacacc cgcagccagg
//
LOCUS       DAOOOG010000226         3439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_33248_length_3439_cov_23.279255, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000226 DAOOOG010000000
VERSION     DAOOOG010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33248_length_3439_cov_23.279255"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="PK242_12400"
     CDS             complement(<1..1723)
                     /locus_tag="PK242_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOZ94862.1"
                     /translation="MGANEFAKLPHIGESLRRKEDVRFLTGAGNYTDDVDQANQKYAV
                     FVRSPHAHARVKGVDTAAAAAMPGVAGVFSGKDIEGKMGGLPCGWLISNPDGSPMKEP
                     PHPILAIGKVRYVGDHVAMVVADTLQQAKDAAEAVQVDYEELAPVIDMRTARNGPALH
                     DEAPDNHCYKWTLGDKAAVDAAFAGAAHVTKLDITNNRLIPNAIEPRAANASYNRATD
                     EYVLYVANQNPHVERLLMTAFVLQLPEHKVRVVAPDVGGGFGSKIFLYAEDVALTWAA
                     KQLNCAIKWTAERSESFVSDAHGRDHITHAEMAMDKDGKFLAMRVHTDANLGAYLSTF
                     ASSVPTILYGTLLAGQYAAPQIYVEVDGWFTSTAPVDAYRGAGRPEATYVVERLVTRC
                     AWELGLSQDEIRKRNFITQFPYQTPVALQYDIGDYHACMNDAQKLADVAGFDARRQAS
                     EAKGLKRGIGYSSYIEACGLAPSNIAGALGARAGLFEAGEVRVHPTGSVTVFTGSHSH
                     GQGHETTFAQLVAARLGIDPSQVDIVHGDTGRVPFGMGTYGSRSLSVGGTAIMKALDK
                     IEAKAKKI"
     gene            complement(1748..2215)
                     /locus_tag="PK242_12405"
     CDS             complement(1748..2215)
                     /locus_tag="PK242_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOZ94863.1"
                     /translation="MKVELKVNGKAVTADVPPNTLLVQLLREHLRLTGTHVGCDTGQC
                     GACTVIADGSAIKACNVLAAQMQGAEVTTIEGLAAADGTLHPMQAAFKECHGLQCGFC
                     TPGMVMSSVDLLARHPQASEAEIRALLDGNLCRCTGYQNIVKAVQTAQKAMAA"
     gene            2343..>3439
                     /locus_tag="PK242_12410"
     CDS             2343..>3439
                     /locus_tag="PK242_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOZ94864.1"
                     /translation="MRHSGRPTGPSHRSPTMMRPDPRQHQIALARQTVLAEGMELTEA
                     LVGAWLDRAWIVQSWRRCLARGARPEAPVVFDAVSPHARARSAEQHAALLAAARPELQ
                     RLAAQIAPIRYFALLTDAQGTVIDTAGAIDHRNRASHAIARVGVDLSEAAIGTSAIGA
                     ALAERQPVWLHRGEHFFADTGVYSCAGAPLFGPDGACLGMIDVTGVNAPERPELKHLV
                     AQSARQIEDALLLARPHQLRLHLAWPAGWQVSGAGSEGAALLCLDADGQVTGANGTAR
                     QMLPALHALHSGPLHASELFALPWASLFDLAAHGQPHTVPLWSGLRVQVRAERSTQPA
                     SAASPGARAAAPRSLKALETELIHQAVRDAGG"
BASE COUNT          456 a         1226 c         1184 g          573 t
ORIGIN      
        1 cgatcttctt ggccttggcc tcgatcttgt ccagcgcctt catgatggcc gtgccaccca
       61 ccgacagcga gcggctgccg taggtgccca tgccgaaagg cacgcggccg gtgtcaccgt
      121 gcacgatatc gacctgcgat gggtctatgc ccaagcgcgc cgccaccagc tgggcaaagg
      181 tggtttcgtg cccctggccg tggctgtgcg agccggtgaa caccgtcacg ctgccggtcg
      241 ggtgcacgcg cacttcgccg gcctcgaaca ggccggcgcg cgcgcccagg gcgccggcga
      301 tgttgctggg cgccagcccg caggcctcga tgtagctgct gtagccgatg ccgcgcttca
      361 ggcccttggc ctcgctggcc tggcggcggg cgtcgaagcc ggccacgtcg gcgagctttt
      421 gcgcgtcgtt catgcaggcg tggtagtcgc cgatgtcgta ctgcagcgcc accggcgtct
      481 ggtaggggaa ctgggtgatg aagttgcgct tgcggatctc gtcctgcgac aggcccagct
      541 cccaggcgca gcgcgtgacc aggcgctcga ccacgtaggt ggcctcgggc cggccggcgc
      601 cgcggtaggc atcgaccggg gcggtgctgg tgaaccagcc gtccacctcg acgtagatct
      661 gcggcgccgc gtactgcccc gccagcaggg tgccgtacag gatggtcggc acgctggagg
      721 cgaaggtgga caggtaggcg cccaggttgg cgtcggtgtg cacgcgcatg gccaggaact
      781 tgccgtcctt gtccatcgcc atttccgcgt gggtgatgtg gtcgcgcccg tgcgcgtcgc
      841 tcacaaacga ttcgctgcgc tcggccgtcc acttgatggc gcagttgagc tgcttggccg
      901 cccaggtgag cgccacgtcc tcggcgtaca gaaagatctt ggagccaaaa ccgccgccga
      961 cgtcgggcgc caccacgcgc accttgtgct cgggcagttg cagcacgaag gcggtcatca
     1021 gcaggcgctc gacgtgcggg ttctggttgg ccacgtacag cacgtattcg tcggtggcgc
     1081 ggttgtagct ggcgttggcg gcgcgcggct cgatggcgtt ggggatcagc cggttgttgg
     1141 tgatgtccag cttggtcacg tgggcggcgc cggcaaaggc ggcgtcgacc gcggccttgt
     1201 cgcccagcgt ccacttgtag cagtggttgt cgggcgcctc gtcgtgcagc gccgggccgt
     1261 tgcgggcggt gcgcatgtcg atcaccggcg ccagctcttc gtagtcgacc tgcaccgcct
     1321 cggcggcgtc cttggcctgc tgcagcgtat cggccaccac catggccacg tggtcgccca
     1381 cgtagcgcac cttgccgatg gccaggatgg ggtgtggcgg ctccttcatg ggcgagccgt
     1441 cggggttgct gatgagccag ccgcagggca gcccgcccat cttgccctcg atgtccttgc
     1501 cgctgaagac gccggccaca ccgggcatgg cggcggcggc ggcggtgtcg acgcccttca
     1561 cgcgggcatg ggcgtgcggc gagcgcacga agacggcgta tttctggttg gcctggtcga
     1621 cgtcgtcggt gtagttgccg gcaccggtca gaaagcgcac gtcttccttg cgcctcagcg
     1681 attcgccgat gtgcggcagt ttggcaaatt cattggcacc catggtcttg tctcctcggt
     1741 ggggccgtca ggcggccatg gccttctggg cggtctgcac cgccttgacg atgttctggt
     1801 aacccgtgca gcggcacagg ttgccgtcca gcagggcgcg gatctcggcc tcgctggcct
     1861 gcgggtggcg cgccagcaga tcgacgctgc tcatcaccat gcccggcgtg cagaaaccgc
     1921 attgcaggcc gtggcattcc ttgaaggccg cctgcatcgg gtgcagcgtg ccgtcggccg
     1981 ccgccagacc ttcgatggtg gtcacctcgg cgccctgcat ctgcgccgcc agcacgttgc
     2041 aggccttgat ggcgctgccg tcggcgatca ccgtgcaggc gccgcactgg ccggtgtcgc
     2101 agcccacgtg ggtgccggtc aggcgcaggt gttcacgtag cagctgcacc agcagcgtgt
     2161 tgggcggcac gtcggccgtg accgccttgc cattgacctt gagctcgact ttcatcgtgg
     2221 gcgtctcctt ttgtcgtggg gtttgtcggt ttgcactgaa agcggtcatt cttggaacaa
     2281 gccgcgccgc tgcacctagg aaataccctc ggttggccgg aagcggctcg aataatctca
     2341 gaatgagaca ctcgggccga cccaccggcc cgtcgcaccg ctcgcccacc atgatgcgcc
     2401 ccgacccccg ccaacaccag atcgccctgg cgcgccagac cgtgctggcc gagggcatgg
     2461 agctgaccga ggcgctcgtc ggcgcctggc tcgaccgcgc ctggatcgtg caaagctggc
     2521 gccgctgcct ggcgcgtggc gcgcggcccg aggcgccggt ggtgttcgac gccgtctcgc
     2581 cccacgcccg cgcccgcagc gccgagcagc acgccgccct gctggccgcc gcccgccccg
     2641 agctgcagcg cctggccgcg cagatcgcgc ccatccgtta tttcgccctg ctgaccgacg
     2701 cgcagggcac cgtcatcgac accgcaggcg ccatcgacca ccgcaaccgc gccagccacg
     2761 ccatcgcgcg cgtgggcgtg gacctgtcgg aagcggccat cggcaccagc gccatcggcg
     2821 cagcgctggc cgagcgccag cctgtgtggc tgcaccgcgg cgaacatttt tttgccgaca
     2881 ccggcgtcta cagctgcgcc ggcgcgcccc tgttcggccc cgacggcgcc tgcctgggca
     2941 tgattgacgt caccggcgtc aacgcgcccg agcgccccga actcaagcac ctggtggccc
     3001 aatccgcccg ccagatcgaa gacgccctgc tgctggcgcg cccgcatcaa cttcgcctgc
     3061 acctggcctg gcccgccggc tggcaggtca gcggcgccgg cagcgagggc gccgccctgc
     3121 tgtgcctgga cgccgacggc caggtcaccg gcgccaacgg caccgcgcgc cagatgctgc
     3181 ccgccctgca cgccctgcac agcggcccgc tgcacgccag cgaactgttt gccctgccct
     3241 gggccagcct gttcgatctc gccgcgcacg gccagccgca cactgtgccc ctgtggtccg
     3301 gcctgcgtgt gcaggtgcgg gccgagcgca gcactcagcc cgccagcgcc gccagccccg
     3361 gcgcgcgtgc cgccgcgcca cgttcactga aagcgctgga aaccgagctg atccaccagg
     3421 ccgtgcgcga cgccggcgg
//
LOCUS       DAOOOG010000227         3431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_33351_length_3431_cov_29.243187, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000227 DAOOOG010000000
VERSION     DAOOOG010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33351_length_3431_cov_29.243187"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PK242_12415"
     CDS             <1..185
                     /locus_tag="PK242_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOZ94865.1"
                     /translation="LVCLDCGHVEEFHDPEIEKRQTAVAKARGFTLVDHALSLYAHCT
                     KANCPHRGSGAPATRR"
     gene            complement(608..1564)
                     /gene="hprK"
                     /locus_tag="PK242_12420"
     CDS             complement(608..1564)
                     /gene="hprK"
                     /locus_tag="PK242_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOZ94866.1"
                     /translation="MKPSAISADVLFEDHRAVLRWQWMAGLEASERRFDEIAVRAARS
                     GADLVGYLNYIHPYRVQVLGEREVAYLTNASSEDCARRIARIVTLEPPVLVLCDGQNP
                     PEALTALCERAHIPLFATQESAAFVVDVLRAYLSRHFADRTTMHGVFMDILGLGVLIT
                     GESGLGKSELGLELISRGNGLVADDAVDLFRINQTTIEGRCPELLRNLLEVRGIGLLD
                     IRAIFGETAVRRHMRLRLIVHLVRRETLERDYERLPYEPLTQEVLGVPIQKVVIQVVA
                     GRNIAVLVEAAVRNHILQLRGIDTYQEFVERHRRAMEQGSSA"
     gene            complement(1617..2093)
                     /locus_tag="PK242_12425"
     CDS             complement(1617..2093)
                     /locus_tag="PK242_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOZ94867.1"
                     /translation="MNRLASILPEAQVLVGVEATSKKRAFEEAGLLFESLHGLSRALI
                     TDSLFARERLGSTGLGHGVAIPHGRIKGLKAPMAAVLRLAQPIGFDAPDEQPVSLLIF
                     LLVPEAATQKHLEILSEIAELLSDASLRERIKGATSAPELHGLIAHWQSGQPVAQA"
     gene            complement(2332..2664)
                     /gene="raiA"
                     /locus_tag="PK242_12430"
     CDS             complement(2332..2664)
                     /gene="raiA"
                     /locus_tag="PK242_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOZ94868.1"
                     /translation="MNMTISGHHLEITPALRSYVSTKLDRVIRHFDQVVDVKVLLSVE
                     NQKEKDKRQRAECRIGVKGNDLFAESSHEDLYAAVDELVDKLDRQVARYKEKQQSHDK
                     NTAVKHLM"
     gene            3008..>3431
                     /locus_tag="PK242_12435"
     CDS             3008..>3431
                     /locus_tag="PK242_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914531.1"
                     /note="responsible for the influx of magnesium ions;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HOZ94869.1"
                     /translation="MLNVFTLSNGRLFQEEIEALESLSRYRPIWVDLEDPTPEEKRWV
                     KQYYGLSIPEDAIDDDLEESARFYEEDNGELHIRSDFLLADSDTPRQLRVAFILNQSN
                     DQLRSHGVLFSIHDESVPAFRLLRMRARRMPGLLEDAKD"
BASE COUNT          571 a         1152 c         1146 g          562 t
ORIGIN      
        1 acctggtgtg cctggactgc ggccacgtgg aggaattcca cgaccccgag atcgagaagc
       61 gccagacggc ggtggccaag gcgcgcggct tcaccctcgt cgaccatgcg ctgagcctgt
      121 acgcgcactg caccaaggcc aactgcccgc accgcggcag cggcgcgccg gccacgcgcc
      181 gctgaatacc tggtcaaaca ggcttccagg cagctcgcag caagcgccaa cagctattta
      241 tttgatagta atctgggggc ctgacgcggc ctggcaacgg tcgtggaacc tagaaccggc
      301 agttggacgc gcccgccctt gccgtgatcg gcgtgccagg caagggtgag cgcggcggct
      361 cccaaggcgc ctacgcgcct tgggacgacg cgaacgggcc cagcctctgg ctgggctgcg
      421 ccctgcaagg gcgacgccgg cgcgggctcc tgcccgcaag aaggagcaac gccgcatggc
      481 gcggcgagcg cagtgcgggt gggcgcgcag cgtgctcacc caggaggtga aggccatggc
      541 agccgccaga ccctgcatcg atgcggtctg cccgaagttg acaagcgatc aactgccgtt
      601 tccaggttca ggcgctgctg ccctgttcca tggcgcgccg gtggcgctcg acgaattcct
      661 ggtaggtgtc gatgccgcgc agctgcagga tgtggttgcg caccgccgcc tccaccagca
      721 cggcgatgtt gcggccggcc accacctgga tcaccacctt ctggatcggc acgcccagca
      781 cctcctgggt cagcggctcg tagggcaggc gttcgtagtc gcgctccagc gtttcgcgcc
      841 gcaccaggtg cacgatcagg cgcagacgca tgtggcggcg cacggcggtt tcgccgaaga
      901 tggcgcgaat gtcgagcagg ccgatgccgc gcacttccag caggttgcgc agcagctcgg
      961 ggcagcggcc ttcgatggtg gtctggttga tgcgaaacag atcgaccgcg tcgtcggcca
     1021 ccaggccgtt gccgcgcgag atcagctcca gccccagctc gctcttgccc aggccggatt
     1081 cgccggtgat cagcacgccc aggcccagga tgtccatgaa cacgccgtgc atggtggtgc
     1141 ggtcggcgaa atggcgcgac aggtaggcgc gcagcacgtc gaccacgaag gccgccgatt
     1201 cctgggtcgc aaacagcgga atgtgcgccc gctcgcacag cgccgtcagt gcctcgggcg
     1261 ggttctggcc gtcgcacagc accagcaccg gcggctccag cgtgacgatg cgggcgatgc
     1321 ggcgcgcgca gtcctcggac gaggcgttgg tcaggtaggc gacctcacgc tcacccagca
     1381 cctgcacacg gtaagggtgg atgtagttca ggtagccgac caggtcggcg ccggagcgcg
     1441 cggcgcgcac ggcgatctcg tcgaagcgcc gctccgacgc ttccagcccc gccatccact
     1501 gccagcgcag tacggcgcgg tggtcttcga acagcacgtc ggcgctgatg gcggaaggct
     1561 tcatggcgtg ggccgcactc ggtctggggc agcgcggacg atggcggcgg cgcgcttcag
     1621 gcctgggcca ccggctggcc cgattgccag tgggcgatca ggccgtgcag ctcgggtgcc
     1681 gaggtggcgc ccttgatgcg ttcgcgcagg ctggcgtcgc tcagcagttc ggcgatttcg
     1741 gacaggattt ccaggtgctt ttgcgtcgcc gcttcgggca ccagcaggaa gatcagcagg
     1801 ctgacgggct gttcgtcggg cgcgtcgaag ccgatcggct gcgccagccg cagcaccgcc
     1861 gccatgggcg ccttcaggcc cttgatgcgg ccgtgcggaa tggccacgcc atggcccagg
     1921 ccggtcgagc ccagccgctc gcgcgcgaac aggctgtcgg tgatgagcgc ccgcgacaga
     1981 ccgtgcaggc tttcgaacag caggccggct tcctcgaacg cgcgcttctt gctggtggct
     2041 tcgacgccga cgaggacctg ggcctccggg aggatggatg cgagacggtt catgggtctg
     2101 gacgcggatt atgcgccgac ggatggccgg tgaccgccgc ggcggcccga aatcgccgcc
     2161 gacgccaaaa atgcacgcgg tgtttcacgc tcgtcgaggg tgttgccgcc cagatgcgcc
     2221 gcgtgcggtc agaccgcggc ggcgcaccgc cgaaaaagaa agccgcctga cgacgtcagg
     2281 acggcttgca atctggagtc tacggtttcc gctcgttatt gttacagcat cctacatcaa
     2341 atgcttgacg gcggtgttct tgtcgtgcga ctgctgcttt tccttgtagc gggcgacctg
     2401 gcgatccagc ttgtcgacca gctcgtccac ggcggcgtac aggtcttcat gactgctctc
     2461 ggcaaacaga tcgttgccct tgacgccgat gcggcattcg gcgcgctgac gcttgtcctt
     2521 ttccttctgg ttctcgaccg acagtaacac cttgacatcc accacctgat cgaagtgccg
     2581 gatcacccga tccagcttgg tgctcacgta gctgcgcagc gcaggcgtga tttccaggtg
     2641 gtgtccgctg atggtcatat tcatgaagaa cctccttctt ccactgacaa aggaccgcgg
     2701 aagcgttccg cgacccgccc gggcggcgcg catggcatgg cttgcccgtc gatgccatgc
     2761 taaaccagtc tgcgccggtt tgccgatcgc ggcaggcgat cgccgtcaag aaactgcccg
     2821 ccccggccag cgccacgcgg cgccggccga tgcgataatc gcaggcttcg atcatcaagc
     2881 acgaaagccc gccttggaac ccgccccgag tcgctagctt tcgtctccct ggcatgcgct
     2941 ggcacggggg gccgaaagcc accgccacct ccgccacgcc gccccgtcaa gcccagggag
     3001 tcctgccatg ctgaacgtat tcacgctgtc caacgggcgg ttgtttcagg aagagatcga
     3061 agcgctggag agcctgtcgc gctaccggcc gatctgggtc gatctggaag accccacgcc
     3121 ggaagaaaag cgctgggtca agcagtacta cggcctgtcc atccccgagg acgccatcga
     3181 cgacgacctg gaagagtcgg cgcgcttcta cgaggaagac aacggcgagc tgcacatccg
     3241 cagcgacttc ctgctggccg actccgacac gccgcgccag ctgcgcgtgg ccttcatcct
     3301 gaaccagtcg aacgaccagc tgcgcagcca cggcgtgctg ttttcgatcc acgacgaatc
     3361 ggtgccggcg tttcgtctgc tgcgcatgcg cgcgcgccgc atgccgggcc tgctggaaga
     3421 cgccaaggac g
//
LOCUS       DAOOOG010000228         3396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_33811_length_3396_cov_27.698593, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000228 DAOOOG010000000
VERSION     DAOOOG010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33811_length_3396_cov_27.698593"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PK242_12440"
     CDS             <1..242
                     /locus_tag="PK242_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ94870.1"
                     /translation="SLFSMVQMLGMSLGVTCAAALLHAFSDWLGGAGAAQSLPAFRAT
                     FVAIGLMTMGSAAIFWQLRPDEPRQPPTGALVDVG"
     gene            complement(634..1230)
                     /locus_tag="PK242_12445"
     CDS             complement(634..1230)
                     /locus_tag="PK242_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ94871.1"
                     /translation="MPRLIFFCGHAGTGKTTLARRAIAALMRQAHARCSGESFCLLDK
                     DTLYGQYSSAVMGVLTGDPNDRDSPPYLQHLRQPEYDGLLATARENLALGVSCIVVGP
                     LSREVRAHRLTDRAWLAVGDDVRVHVVWVHLEEAEARRRITARGNPNDAWKLAHWDAY
                     RTRLFVPTAAEYPELLQYDNTDAPPAAFEGLLEALADR"
     gene            complement(1265..1822)
                     /locus_tag="PK242_12450"
     CDS             complement(1265..1822)
                     /locus_tag="PK242_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOZ94872.1"
                     /translation="MKVLKLSAQGLPQSWISLEQAVIHYAAEEVRWEAGGQIAVFRGG
                     HNAITGEQSIVRINSIIGTHGAPRINPFELRPSLTNAKLFARDRNLCAYCGQHYTEAE
                     LTREHVVPQAQRGRDHWMNVVTACRACNHRKSSRTPEQARMPLLYAPYVPSLWEDFIL
                     RNRRILADQMEFLMAHLPRSSRLHA"
     gene            2001..3014
                     /locus_tag="PK242_12455"
     CDS             2001..3014
                     /locus_tag="PK242_12455"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOZ94873.1"
                     /translation="MKIFRGLRHPGMAPGCALTIGNFDGVHRGHQAMLALLRSEAAHR
                     GVPSCVMTFEPHPRDYFAAAQHKPELAPARIATFRDKLAELARCGIDQAVVLPFNQAL
                     ASQPPQDFIDQVLVQGLGVRYVLVGDDFRFGARRAGDYAMLDAAGQRLGFDVARMLSY
                     EVHGQRVSSSAVREALAAGDMAAAAALLGRPYAISGHVVHGRKLGRALAEAAPGRGDG
                     FRTLNLRFSRRAHAWKPAASGIFVVEVHGLDARPLPGVANLGVRPSLDPHDVNGGRVL
                     LETHVLDWPARLGPEGAYGKIIRVDLLHKLHDELRYHSLAALTEGIARDCAAARDWLA
                     ARI"
     gene            3137..>3396
                     /locus_tag="PK242_12460"
     CDS             3137..>3396
                     /locus_tag="PK242_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOZ94874.1"
                     /translation="MSTDAKTDYRQTLNLPDTPFPMRGDLPKREPGWVQAWEDQGLYR
                     RLRAARAGRAKFILHDGPPYANGQIHIGHAVNKVLKDMIVKAR"
BASE COUNT          485 a         1204 c         1182 g          525 t
ORIGIN      
        1 acagcctgtt ctcgatggtg cagatgctgg gcatgagcct gggcgtgacc tgcgccgccg
       61 ccctgctgca cgccttcagc gactggctgg gtggcgccgg cgccgcgcag tcgctgccgg
      121 cgtttcgtgc caccttcgtg gcgatcgggc tgatgaccat gggctcggcg gcgatcttct
      181 ggcaactgcg ccccgacgag ccgcgccagc cgccgacggg cgcgctggtg gacgtgggtt
      241 gagccgcgtc acacccctgc tgcgttccac cagtggctca gcatatgggc ggcgccggtc
      301 tgaccgcggt ctcgtccatc atcgaagccc agcgaggtca ttcgccggtt gtcatcccgc
      361 aagcggccgt tggacgtgcc tgcccgcgcc gtggtcggcg cgccaggcaa ggatgagcgc
      421 cgctgccccg aaggcgcctg cgcgcctttg gacggcgcgc tcggggcggg cgggtgcgca
      481 gcgtgctcac ccaggaggtg aaggccatcg cagccgccag accccgcatc gatgcggtct
      541 gcccgaagtt gacaggcggt caactgccgt ttccaggata agtgcctgac agcgcgaaac
      601 actgcaccac ggcagcgctc cagcagcgag cgcctatcga tccgccagcg cctccagcag
      661 cccctcgaag gccgcgggcg gcgcgtcggt gttgtcgtac tgcagcagct ccggatactc
      721 ggccgccgtc ggcacgaaca gccgcgtgcg gtaggcgtcc cagtgcgcca gcttccaggc
      781 gtcgttggga ttgccgcggg cggtgatgcg ccggcgcgcc tcggcttctt ccagatgcac
      841 ccacaccacg tgcacccgca cgtcgtcgcc caccgccagc caggcgcggt cggtcaggcg
      901 gtgggcccgc acttcgcgcg acagcgggcc gaccacgatg cagctcacgc ccagcgccag
      961 gttctcgcgc gcggtggcca gcagaccgtc gtactcgggc tgacgcaggt gctgcaggta
     1021 gggcgggctg tcgcggtcgt tcgggtcgcc ggtcagcacg cccatcacgg cgctgctgta
     1081 ctggccgtac agcgtgtcct tgtccagcag gcagaacgat tcgccgctgc agcgcgcatg
     1141 ggcctgccgc atcagcgccg cgatggcgcg acgcgccagg gtggtcttgc cggtgccggc
     1201 gtggccgcaa aagaagatca ggcgtggcat ggggcgagca tagccgcgcc ggcggcccgc
     1261 cccgtcaggc gtgcaggcgc gagctgcgtg gcaggtgggc catcaggaac tccatctggt
     1321 cggccaggat gcggcggttg cgcaggatga agtcttccca caggctcgga acgtagggcg
     1381 catacagcag cggcatgcgg gcctgctcgg gcgtgcggct gctcttgcgg tggttgcagg
     1441 cgcggcaggc ggtgaccacg ttcatccagt ggtcgcgccc gcgctgcgcc tgtggcacca
     1501 cgtgctcgcg cgtcagttcg gcctcggtgt aatgctggcc gcagtaggcg cacaggttgc
     1561 ggtcgcgcgc gaacagcttg gcgttggtca ggctggggcg cagctcgaag gggttgatgc
     1621 gcggcgcgcc gtgggtgccg atgatgctgt tgatgcgcac gatcgactgc tcgccggtga
     1681 tggcgttgtg gccaccgcgg aacaccgcga tctggcctcc cgcttcccag cgcacttctt
     1741 cggcggcgta gtggatcacc gcctgctcca gcgagatcca cgattgcggc agcccctgtg
     1801 ccgacagctt gagcaccttc acaaaacgac ctcctgacac ggaaaccctg tttgtgtggc
     1861 cggcattgcc agggccggcc gccctgtccg tggcgcgacg gcgccccttc gaacaactcg
     1921 caatatactc cgatttcaca atacttttgc gcgcttccgc ttgtctggca agcggaaggc
     1981 gctatcaaaa acgtaccgac atgaagatct tccgcggcct gcgccacccc ggcatggcac
     2041 ccggctgcgc cctgaccatc ggcaatttcg acggcgtgca ccgcggccac caggccatgc
     2101 tggcgctgct gcgcagcgaa gcggcgcacc gcggcgtgcc cagctgcgtg atgaccttcg
     2161 agccgcaccc gcgcgactac tttgccgccg cgcagcacaa gcccgagctg gcaccggcgc
     2221 gcatcgccac cttccgcgac aagctggccg agctggcgcg ctgcggcatc gaccaggcgg
     2281 tggtgctgcc gttcaaccag gcgctggcca gccagccacc gcaggacttc atcgaccagg
     2341 tgctggtgca gggcctgggc gtgcgctatg tgctggtggg cgacgacttc cgcttcggcg
     2401 cccggcgcgc cggcgactac gccatgctgg acgccgccgg ccagcggctg ggcttcgacg
     2461 tggcgcgcat gctgagctac gaggtgcacg gccagcgcgt gtccagctcg gcggtgcgcg
     2521 aggcgctggc ggccggcgac atggccgccg ccgccgcgct gctgggacgg ccctacgcca
     2581 tcagcggcca cgtggtgcat gggcgcaagc tgggccgcgc gctggccgaa gcggcgccgg
     2641 gccgcggcga cggctttcgc acgctgaacc tgcgcttttc gcgccgcgcc cacgcctgga
     2701 agccggcggc cagcggcatt ttcgtggtcg aggtgcatgg gctggacgcc cgccccctgc
     2761 ccggcgtggc caacctgggg gtgcgcccct cgctcgatcc gcacgacgtc aacggtggcc
     2821 gcgtgctgct ggaaacccat gtgctggact ggccggcccg gctggggccg gagggggcct
     2881 acggtaaaat catccgcgtg gatctgctgc acaaactgca cgacgaattg cgctatcaca
     2941 gcctggcggc gctgaccgaa ggcatcgcgc gcgattgcgc cgccgcccgc gactggctgg
     3001 cggcgcgaat ttgaaggggc ggcgggcgcc ccgcccgcca catgccccgc tccttatttg
     3061 atagctgctt gcgcacgtcc tgcctgcgca acaggccgat cggatccata ccccccgttt
     3121 tctccctctg cacgccatga gcaccgacgc caagaccgac taccgccaga cgctcaatct
     3181 gcccgacacg cccttcccga tgcgcggcga cctgcccaag cgcgagccgg gctgggtgca
     3241 ggcctgggaa gaccaggggc tgtaccggcg cctgcgcgcg gcgcgcgccg ggcgcgcgaa
     3301 gttcatcctg cacgacggcc cgccctacgc caacggccag atccacatcg gccacgcggt
     3361 caacaaggtg ctcaaggaca tgatcgtcaa ggcgcg
//
LOCUS       DAOOOG010000229         3378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_34061_length_3378_cov_27.649714, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000229 DAOOOG010000000
VERSION     DAOOOG010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34061_length_3378_cov_27.649714"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..91
                     /locus_tag="PK242_12465"
     CDS             <1..91
                     /locus_tag="PK242_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ94875.1"
                     /translation="SRAAEWLGMNRNTLRKKLIEHGLMAGEKS"
     gene            158..1786
                     /gene="purH"
                     /locus_tag="PK242_12470"
     CDS             158..1786
                     /gene="purH"
                     /locus_tag="PK242_12470"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOZ94876.1"
                     /translation="MTTALISVSDKTGIVDFAHALHVLGVRLLSTGGTARLLRDAGLP
                     VTEVADHTGFPEMLDGRVKTLHPRIHGGLLARRDLLEHMAALKTHGIATIDLLVVNLY
                     PFEATVARPDCTLADAIENIDIGGPAMVRSAAKNWQGVAVLTDASQYDGVLAELKAGG
                     ISQKTRFQLAVAAFNRISDYDGAISDYLSAIDFEAGGADRVPVRSLFPAQSNGRFVKL
                     QDLRYGENAHQQAALYRDLHPAPGAIVSAQQLQGKELSYNNIADADAAWECVKQWATG
                     ASATPGQAAACVIVKHANPCGVAVGESALAAYQKAFKTDPTSAFGGIIAFNVPLDGAA
                     AEAVSKQFVEVLMAPGFTPEARAVFQAKPNVRLLQIDLSGVQPGGATPWARGQNAIDV
                     KRVGSGLLMQTADHHELKAADLKVVTKKQPTPAQIEDLLFAWKVAQYVKSNAIVFCAG
                     GMTLGVGAGQMSRLDSARIASIKAEAAGLSLQGSAVASDAFFPFRDGLDVVIDHGAAC
                     VIQPGGSMRDQEVIDAADERGVAMVFTGVRHFRH"
     gene            complement(1758..2636)
                     /locus_tag="PK242_12475"
     CDS             complement(1758..2636)
                     /locus_tag="PK242_12475"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOZ94877.1"
                     /translation="MRALLTGTLAVALLTAYTLLLFALLAPLILLKLLTRPLPPIAPL
                     DAAVVAIADTWNRLNAAGMQWASGTRWQLSLPEGLSRQAWYLVICNHQSWVDILVLQR
                     SLQGRAPFLKFFIKRELIWVPLVGLVWWALDFPFLRRRGGASGAQDLATARAACARFR
                     ALPTSVMSFVEGTRFTPAKQQAQQSPFRHLLRPKAGGVMSALEVLGDRLDAVLDVTIV
                     YPGQVPRFIDVLCGRVPHVRVHVERLPVPLAADGGPLDTAALQAWLGDRWQAKDARFA
                     QLASGHPLSGGSAAPR"
     gene            complement(2696..>3378)
                     /locus_tag="PK242_12480"
     CDS             complement(2696..>3378)
                     /locus_tag="PK242_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ94878.1"
                     /translation="GNMNFVRASVDFTRRCRELCTQHGALLIYDEVMTGFRVALGGAQ
                     SVYAAAIPGFEPDLTVMGKVIGGGMPLAAFGGKRAVMEQLAPLGPVYQAGTLSGNPVA
                     TACGLATLREIRKPGFFEALTARTQRLVDGLVAAARAAGVPFSADSEGGMFGFFLLPE
                     LPRNYGEVMKSDGARFNRLFHGLLERGVYIAPALYEAGFVSSAHSDADIDATIDAARA
                     VLAQLGQH"
BASE COUNT          576 a         1178 c         1157 g          467 t
ORIGIN      
        1 gagccgcgcc gccgaatggc tgggcatgaa ccgcaacacc ctgcgcaaga agctgatcga
       61 gcacggcctg atggctggcg agaaatcctg aattgatagc gtcttgcgca agcagcacaa
      121 gcgctggagc gatttttgac ttgaagaaag tgcgtccatg accaccgccc tgatctccgt
      181 ctccgacaag accggcattg ttgatttcgc gcatgcgctg cacgtgctgg gcgtgcgcct
      241 gctgtccacc ggcggcaccg cccgcctgct gcgggacgcg ggcctgcccg tcaccgaggt
      301 ggccgatcac accggattcc ccgaaatgct cgatggccgc gtcaagaccc tgcaccccag
      361 gatccacggc ggcctgctgg cgcggcgtga cctgctggag cacatggcgg cgctcaagac
      421 ccacggcatc gccaccatcg acctgctggt ggtcaacctc tacccgttcg aggccacggt
      481 ggcccggccc gactgcacgc tggccgacgc catcgaaaac atcgacatcg gcggcccggc
      541 catggtgcgc agcgcggcca aaaactggca gggcgtggcg gtgctgaccg acgcctcgca
      601 atacgatggc gtgctggccg agctgaaggc cggcggcatt agccagaaga ccaggttcca
      661 gctggcggtg gctgccttca accgcatcag cgactacgac ggcgccatca gcgactacct
      721 gtctgctatt gatttcgaag ctggtggcgc tgaccgggtg cccgtcagaa gcctttttcc
      781 tgcacagagc aacggccgct tcgtgaagct ccaggacctg cgctacggcg agaacgcgca
      841 ccagcaggcc gccctgtacc gcgacctgca cccggcgccc ggcgccatcg tcagcgcgca
      901 gcagctgcag ggcaaggagc tgtcctacaa caacatcgcc gacgccgacg cggcctggga
      961 atgcgtcaag cagtgggcca cgggagcgtc agcgacccca ggtcaggcgg cggcctgcgt
     1021 catcgtcaag cacgccaacc cctgcggcgt ggccgtgggc gagagcgcac tggcggcgta
     1081 tcagaaggct ttcaagaccg atcccaccag cgctttcggc ggcatcatcg ccttcaacgt
     1141 gccgctcgac ggcgcggccg ccgaggcggt gagcaaacag ttcgtcgagg tgctgatggc
     1201 gcccggcttc acccccgagg cgcgggcggt gtttcaggcc aagcccaacg tgcgcctgct
     1261 gcagatcgat ctgagcggcg tgcagccggg tggcgccacg ccctgggcgc gcggccagaa
     1321 cgccatcgac gtcaagcgcg tcggctcggg cctgctgatg cagaccgccg accaccacga
     1381 gctcaaggct gccgacctga aggtggtgac gaagaagcag cccacgccgg cgcagatcga
     1441 agacctgctg tttgcctgga aggtggcgca atacgtcaag agcaacgcca tcgtgttctg
     1501 cgccggtggc atgacgctgg gcgtgggcgc cgggcagatg agtcggctgg attcggcgcg
     1561 catcgccagc atcaaggccg aagcggcggg cctgtcgctg cagggctcgg cggtggccag
     1621 cgacgccttc tttccgttcc gcgacggact ggacgtggtg atcgaccacg gcgcggcctg
     1681 cgtcatccag ccgggcggct cgatgcgcga tcaggaagtg atcgacgctg ccgacgagcg
     1741 cggcgtggcc atggtgttca ccggggtgcg gcacttccgc cactgagcgg gtggccggac
     1801 gcgagctgcg caaagcgcgc gtccttggcc tgccagcgat cacccagcca ggcctgcagc
     1861 gcggccgtat ccagcggccc gccatctgcg gccagcggca ccggcagccg ctcgacgtgc
     1921 acgcgcacgt gcggcacgcg gccgcacagc acgtcgatga agcgcggcac ctggccggga
     1981 tagacgatgg tcacgtccag cacggcgtcg agccggtcgc ccagcacctc cagcgcgctc
     2041 atcaccccgc cggccttggg gcgcagcagg tgccggaagg gcgactgctg ggcctgctgc
     2101 ttggccggcg tgaagcgcgt gccctcgacg aagctcatca ccgacgtcgg cagcgcgcgg
     2161 aagcgggcgc aggcggcgcg cgccgtggcc aggtcctgcg ccccgctggc accgccgcgc
     2221 cggcgcagga agggaaagtc cagcgcccac cacaccaggc ccaccagcgg cacccagatc
     2281 agctcgcgct tgatgaagaa cttgagaaac ggcgcgcgcc cctgcaggct gcgttgcagc
     2341 accagaatgt cgacccagct ctgatggttg cagatcacca ggtaccaggc ctggcggctc
     2401 agcccttccg gcaggctgag ttgccagcgc gtgccactgg cccactgcat gccggcggcg
     2461 ttgaggcggt tccaggtgtc ggcaatcgcc accacggcgg cgtccagcgg cgcgatcggt
     2521 ggcagcgggc gcgtcagcag cttcagcaga atcagcggcg ccagcagcgc aaacagcagc
     2581 agcgtgtacg ccgtcagcag cgccacggcg agggtgccgg tcaggagcgc acgcacggca
     2641 ggtcggtcag cagaagcggc cagaaggtgg gcatgcgggg cccgttcaca ccttctcagt
     2701 gctggcccag ctgcgccagc accgcgcgcg ccgcgtcgat ggtggcgtcg atgtcggcgt
     2761 cgctgtgcgc gctgctgacg aagcccgcct catacagggc cggcgcgatg tacacgccgc
     2821 gctccaacag gccgtggaac agtcggttga agcgcgcacc gtcggacttc atcacttcgc
     2881 cgtagttgcg cggcagctcc ggcagaagga agaaaccgaa catgccccct tcggaatcgg
     2941 ccgaaaacgg cacgccggcg gcacgcgcgg cggccaccag gccatcgacc aggcgctgcg
     3001 tcctggccgt gagcgcctcg aagaagccgg gcttgcggat ttcgcgcagc gtggccaggc
     3061 cgcaagcggt ggccaccggg ttgccgctca gcgtgccggc ctgatacacc gggcccagcg
     3121 gggccagctg ctccatcacc gcgcgcttgc cgccgaaggc cgccagcggc atgccgccgc
     3181 cgatcacctt gcccatcacc gtcaggtcgg gctcgaagcc ggggatggcc gccgcataca
     3241 cgctctgcgc gccgcccagc gcgacgcgaa agccggtcat cacctcgtcg tagatcaaga
     3301 gcgcgccatg ctgcgtgcac agttcgcggc agcggcgcgt gaagtcgacg ctggcgcgca
     3361 cgaagttcat gttgccgg
//
LOCUS       DAOOOG010000230         3264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_35704_length_3264_cov_29.888127, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000230 DAOOOG010000000
VERSION     DAOOOG010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35704_length_3264_cov_29.888127"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1961)
                     /gene="dnaG"
                     /locus_tag="PK242_12485"
     CDS             complement(147..1961)
                     /gene="dnaG"
                     /locus_tag="PK242_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOZ94879.1"
                     /translation="MAIPQHFLQELLARADIVEIVGRYVPLKKTGANFSGLCPFHGEK
                     TPSFTVSPSKQFYHCFGCGKNGNAIGFLMDHTGAGFVETVHELAQQYGLQVPEDERSP
                     AERARAAEQTEQRASLTDVLARAADAWRKQLKASPRAIDYLKRRGLSGQVARQFGLGY
                     APEGWRGLASVFPDYADPLLVESGLVIVSEDDEGKRYDRFRDRIMFPIRSVKGEVIGF
                     GGRVLGDEKPKYLNSPETPVFHKGRELYGLFEARTALREQGYALVVEGYMDVVALAQQ
                     GLGNAVATLGTACTPEHVHKLFRFTDHIVFSFDGDAAGRRAAHKALEAALPLATDTRS
                     VKFLFLPPEHDPDSFVRAEGVEAFAQAVRDAVPLSRFLLDAAAEDCDLQTAEGRSRFA
                     ARARPLWQLLPTGALAAQLLGDIAAQVQIGTHELEQLWGLRRTPPARSENNSYSRSPH
                     KAREPKTPARAVAVRHAPRRAPSPRVSRALQIVLTEPSAWDRLTQSDQGLLCDLPAPL
                     GALFTWLAGQHLEHGPQPWSALREALRGHGHEAFAVELLERLPPDIESDAEELSRILT
                     LEHDARYAAERQRLAAAAAAGDTAAYARLREMPVTRPR"
     gene            2130..>3264
                     /locus_tag="PK242_12490"
     CDS             2130..>3264
                     /locus_tag="PK242_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ94880.1"
                     /translation="MPSIFDTDLPRNPANHAALTPLAFIQRTAELYPGRLAIVHGELR
                     QSWGQTYARCRQLASSLQRHGIGRNDTVAVMLPNTPPMVEAHFGVPMAGAVLNTLNTR
                     LDPEAVAFMLDHGEAKAIIVDPEFAGIMKKALALRQSKAPLLVIDVEDALYGPGQPIG
                     SIGYEAFVAQGDAGFEWDYPGDEWDAIALNYTSGTTGNPKGVVYHHRGAAINAISNIL
                     EWDMPKHAVYLWTLPMFHCNGWCFPWTVAARAGVNVCLRRVEARAIFDAIRQHGVTHY
                     CGAPIVHGLLVNAPDELKAGVPSGVKCMVAGAAPPASMIEGMERLGFDLTHVYGLTEV
                     YGPATVCAKHEAWEQLAIGERAALNARQGVRYHLQRDARVLDPE"
BASE COUNT          487 a         1094 c         1169 g          514 t
ORIGIN      
        1 aggactcccc aaaggggaca tctctatttg ggctgaaagg ggacatttct attttttggg
       61 ttgacactca tttcaaggac ccatttcaag gtccccaagg gattggcgac gccgccgcgc
      121 ccgccgtcga tggggagtgg ttcggttcag cgcgggcggg tgaccggcat ttcacgcaac
      181 cgggcgtagg cggcggtgtc tccggcagcc gcggcggcgg ccaggcgctg gcgctcggcg
      241 gcgtagcggg cgtcgtgctc cagcgtcagg atgcgcgaca gttcttcggc gtcgctttcg
      301 atgtcgggcg gcaggcgctc cagcagctcg acggcaaagg cctcgtggcc gtggccgcgc
      361 agggcttcgc gcagcgcgct ccacggctgc ggaccgtgct ccaggtgctg gccggccagc
      421 caggtgaaca gcgcgcccag gggcgccggc aggtcgcaca gcaggccctg atcgctttgc
      481 gtgaggcgat cccaggcgct cggctcggtc agcacgatct gcagggcacg gctgacgcgc
      541 ggcgagggtg cgcgccgcgg cgcgtggcgg acggccacgg ctcttgcggg tgtttttggc
      601 tctcttgcct tatggggtga gcgcgaatag ctattgtttt cagagcgtgc cgggggcgtt
      661 cgacgcaagc cccatagttg ctccagctcg tgcgtgccga tctgcacctg cgcggcgatg
      721 tcgcccagca gctgtgccgc cagcgccccc gtgggcagca actgccacag tggccgggct
      781 cgggccgcga agcgcgagcg gccttcggcg gtctgcaggt cgcagtcctc ggccgcggcg
      841 tccagcagga agcgcgacag cggcacggcg tcacgcaccg cctgggcgaa ggcctcaacc
      901 ccctcggcac gcacgaagct gtcggggtcg tgctcgggcg gcagaaacag gaacttcacg
      961 ctgcgggtgt cggtggccag gggcagggcg gcttccagcg ccttgtgggc cgcgcgtcgg
     1021 ccggcggcgt cgccgtcgaa gctgaacacg atgtggtcgg tgaagcgaaa cagcttgtgc
     1081 acgtgctcgg gcgtgcaggc tgtgcccagg gtggcgacgg cgtttcccag gccctgctgc
     1141 gccagcgcca ccacgtccat gtagccttcc accaccagcg cgtagccctg ctcgcgcagc
     1201 gcggtgcgcg cttcgaacag gccgtacaac tcgcggccct tgtgaaacac cggggtttcg
     1261 ggagagttca ggtatttggg cttttcgtcg cccagcacgc ggccaccgaa gccgatcact
     1321 tcgcccttga ccgagcggat ggggaacatg atgcggtcgc ggaaacggtc gtagcgcttg
     1381 ccctcgtcgt cctccgacac gatgaccagc ccgctttcca ccagcagggg gtcggcgtag
     1441 tccgggaaca cgctggccag gccgcgccag ccctcgggcg cgtagcccag gccgaactgg
     1501 cgcgccacct ggcccgacag gccgcgccgc ttgaggtagt cgatggcgcg cggcgaggcc
     1561 ttgagctgct tgcgccaggc gtcggcggcg cgggccagca cgtcggtcag gctggcgcgc
     1621 tgctcggtct gctcggcggc gcgggcgcgc tcggccgggc tgcgttcgtc ctcgggcacc
     1681 tgcaggccgt attgctgggc cagttcgtgc accgtctcga cgaagccggc gccggtgtgg
     1741 tccatcagaa agccgatggc gttgccgttc ttgccgcagc cgaagcagtg gtagaactgc
     1801 ttggacgggc tgacggtgaa cgacggcgtc ttttcgccat ggaacggaca caggccggaa
     1861 aaattggcgc cggtcttctt gagcggcacg tagcggccga cgatctcgac gatgtcggcg
     1921 cgcgccagga gctcctggag aaagtgctgc ggaatggcca cgaggctatt ttgcccgggc
     1981 catctcgggg acgtcaggcg atcgagccga tcgacgtgcc gcgcgctgcg gggttccggg
     2041 caggcacggg accggcgcca gctgcccgtc aggccgctga ctatgatcgg gcgtcccgcc
     2101 cccgcgcgtt ccctttccag gagacaggca tgcccagcat cttcgacacc gatctgcctc
     2161 gcaacccggc caaccatgct gcactgacgc cgctggcctt catccagcgc acggccgagc
     2221 tgtatccggg ccgcctggcc atcgtgcatg gcgagctgcg ccagagctgg ggccagacct
     2281 atgcgcgctg tcgccagctg gccagcagcc tgcagaggca cggcatcggc aggaacgaca
     2341 ccgtggccgt gatgctgccc aacaccccgc ccatggtcga ggcgcatttc ggcgtgccga
     2401 tggccggcgc cgtgctcaac acgctcaaca cccggctcga ccccgaggcg gtggccttca
     2461 tgctggacca cggcgaggcc aaggcgatca tcgtcgaccc cgagttcgcg ggcatcatga
     2521 agaaggcgct ggcgctgcgc cagtcgaagg cgccgctgct ggtgatcgac gtcgaggacg
     2581 cgctgtacgg cccggggcag ccgatcggca gcatcggcta cgaggccttc gtggcgcagg
     2641 gcgacgccgg cttcgagtgg gactatccgg gcgacgagtg ggacgccatc gcgctcaact
     2701 acaccagcgg caccaccggc aaccccaagg gcgtggtcta ccaccaccgc ggcgcggcca
     2761 tcaatgccat cagcaacatc ctcgaatggg acatgcccaa gcacgcggtc tatctgtgga
     2821 cgctgccgat gttccactgc aacggctggt gctttccgtg gacggtggcg gcgcgcgccg
     2881 gtgtgaacgt gtgcctgcgc cgggtcgagg cgcgggcgat cttcgacgcc atccgccagc
     2941 atggcgtgac gcactactgc ggcgcgccca tcgtgcacgg cctgctggtc aacgcaccgg
     3001 acgagctgaa ggccggtgtg cccagcggcg tcaaatgcat ggtggcgggc gcggcgccgc
     3061 cggcctcgat gatcgagggc atggagcgcc tggggttcga tctgacccac gtctacggcc
     3121 tgaccgaggt ttatggcccg gccaccgtgt gcgccaagca cgaggcgtgg gagcaactgg
     3181 ctattggcga gcgcgcggcg ctgaacgcgc gccagggcgt gcgctaccac ctgcagcgcg
     3241 acgcgcgcgt gctcgacccc gaga
//
LOCUS       DAOOOG010000231         3240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_36053_length_3240_cov_29.358242, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000231 DAOOOG010000000
VERSION     DAOOOG010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36053_length_3240_cov_29.358242"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="dctP"
                     /locus_tag="PK242_12495"
     CDS             complement(<1..656)
                     /gene="dctP"
                     /locus_tag="PK242_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOZ94881.1"
                     /translation="MKLRRTLLSVAAATSAALALGLPATVQAQNYKSEYRMSLVVGTA
                     FPWGKGGEIWANKVREKTNGRINIKLYPGVSLIQGDQTREFSALRQGVIDMAIGSTIN
                     WSPQVKQLNLFSLPFLMPDYAAIDALTQGAVGKEIFKILDKAGVVPLAWGENGYRELT
                     NSKRPVRTPADLKGLKIRVVGSPLFLDTFTALGANPTQMSWADAQPAFASGAVDGQEN
                     P"
     gene            complement(736..2034)
                     /locus_tag="PK242_12500"
     CDS             complement(736..2034)
                     /locus_tag="PK242_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOZ94882.1"
                     /translation="MIATLLFTLFIVTMLMGVPIGASLGLAGAAAIALANAQTPWFGL
                     LAVPQNFYAGLGKYPLLAIPMFVLVGSIFDRSGVAARLVNFATAIVGRGPGMLPLIAI
                     AVAMFMGGISGSGPATAAAVGSVMIAAMARAGYPGAYSASVVGAAAATDILIPPSVAF
                     IVYSVLVPGASVPALFAAGMVPGLLVGLALIAPAVWMARRHGMGAREADMPRPPFWRS
                     LREAVWGLAAPVVILGGMRMGWFTPTEAAVVAVFYGLFVGMVVHRTMGVRDLFPILKE
                     SGELSAVILIVVSLAGIFAFSLSTLGVIDPVTQAIVHSGLGEYGVLTLIVLLLVVAGM
                     FLDGISIFLIFVPLMWPIMQHYQWDVVWFGVLLTLMVAMGQFTPPLAVNLMVSCRIAG
                     VSMEQTVRWVLPLVLAMGVVVLAVIVWPPLALWLPQALGF"
     gene            complement(2249..2830)
                     /locus_tag="PK242_12505"
     CDS             complement(2249..2830)
                     /locus_tag="PK242_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOZ94883.1"
                     /translation="MSLPADSPPPQGADPTAPAGAATRVPLAIEDWLTVIIMALLALI
                     TFVNVLVRYFTDASFAWTEEVSIFLMIVLAMVASSAAVARGRHIRIEYFADAGPVSRQ
                     RALARLGAALVALLFGLLAVLSARVLWDDVQYGETSPGIGVPQWWYTMWLPILSLAIM
                     LRAIGLWWRGGQPAADNPELPTSPAVRDEERAP"
BASE COUNT          538 a         1170 c         1118 g          414 t
ORIGIN      
        1 gggttttcct gcccgtccac cgcgccgctg gcgaacgctg gctgcgcgtc ggcccagctc
       61 atctgcgtcg ggttggcgcc cagggcggtg aaggtatcca ggaacagcgg cgagccgacc
      121 acgcggatct tcaggccctt gaggtcggcc ggcgtgcgca ccgggcgctt ggagttggtc
      181 agttcgcggt agccgttttc gccccaggcc agcggcacca cgccggcctt gtcgaggatc
      241 ttgaagattt ccttgccgac cgcgccttgc gtcagcgcgt cgatggcggc gtagtcgggc
      301 atcaggaagg gcagcgagaa caggttgagc tgcttgacct gcggcgacca gttgatggtg
      361 gagccgatcg ccatgtcgat cacgccctgg cgcagcgccg agaactcgcg cgtctggtcg
      421 ccctggatca gcgacacgcc ggggtagagc ttgatgttga tgcggccgtt ggttttttcg
      481 cgcaccttgt tggcccagat ctcgccgccc ttgccccagg ggaaggcggt gcccaccacc
      541 agcgacatgc ggtactcgct cttgtagttc tgtgcctgca ccgtggcggg caggcccagg
      601 gccagggcgg ccgaggtggc agcggccacg gacagcaggg ttcgacgcag tttcatgaca
      661 gggtctcctt tacggttcac tatgaaaaaa agagctgctt gcgcaggttg gtaaaagctt
      721 tgaagccgtt ttgactcaga atccgagcgc ctggggcagc cacagcgcga gcggcggcca
      781 gacgatcacc gccagcacca ccacgcccat ggccagcacc agcggcagca cccagcgcac
      841 cgtctgttcc atgctgacgc cggcgatgcg gcacgacacc atcaggttga ccgccagcgg
      901 cggcgtgaac tggcccatgg ccaccatcag cgtcagcagc acgccgaacc acaccacgtc
      961 ccactggtag tgctgcatga tgggccacat cagcggcacg aagatcagga agatcgagat
     1021 gccgtccagg aacatgccgg ccaccaccag cagcagcacg atcagcgtca gcacgccgta
     1081 ttcgcccagg ccggaatgca cgatggcctg ggtgaccggg tcgatcacgc ccagggtgga
     1141 gagcgagaag gcgaagatgc cggccagcga caccacgatc aggatcaccg ccgacagctc
     1201 acccgattcc ttgaggatgg gaaacagatc gcgcacgccc atggtgcggt gcaccaccat
     1261 gcccacgaac agaccgtaga acaccgccac cacggccgcc tcggtgggcg tgaaccagcc
     1321 catgcgcatg ccgcccagga tcaccaccgg cgccgccagc ccccacaccg cctcgcgcag
     1381 gctccgccag aacggcgggc gcggcatgtc ggcctcgcgc gcgcccatgc cgtggcggcg
     1441 cgccatccac accgccggcg cgatcagcgc cagccccacc agcaggcccg gcaccatgcc
     1501 ggcggcaaac agcgccggca ccgaggcgcc cggcaccagc accgaataga cgatgaaggc
     1561 caccgagggc gggatcagga tgtcggtggc cgccgccgcc cccaccacgc tggccgaata
     1621 ggcgccggga tagccggcgc gcgccatggc ggcgatcatc acgctgccca cggccgccgc
     1681 cgtggccggc cccgagcccg aaatgccgcc catgaacatc gccaccgcga tggcgatcag
     1741 cggcagcatg cccggcccgc gcccgacgat ggcggtggca aagttgacca gccgcgcggc
     1801 cacgcccgag cggtcgaaga tcgagcccac cagcacgaac atcgggatcg ccagcagcgg
     1861 gtactttccc agcccggcgt agaagttctg cggcaccgcc agcaggccga accagggcgt
     1921 ctgggcgttg gccagcgcga tcgccgccgc ccccgccagc cccagcgacg caccaatcgg
     1981 cacgcccatc agcatggtga cgatgaacag ggtgaacagc agggtggcga tcatggaatg
     2041 aggcccccac gctcccccgc tgcgcgaggt gcgctgcccc ccgagggggc cgcaggccgc
     2101 cttgggagcg gcccggcggc ggcctgagtg cccccacgct cccccgctgc gcgaggtgcg
     2161 ctgccccccg agggggccgc aggccgcctt gggagcggcc cggcggcggc ctgagcgctc
     2221 cggcgctttt ctgctgtgcg aggtgtgttc atggcgcgcg ctcctcgtcg cgcacggcgg
     2281 gcgaggtggg cagctcgggg ttgtcagccg ccggctggcc gccgcgccac cacaggccga
     2341 tggcgcgcag catgatggcc aggctgagga tcggcagcca catggtgtac caccactgcg
     2401 gcacgccgat gccgggcgag gtttcgccgt actgcacgtc gtcccacagc acgcgcgccg
     2461 acagcacggc cagcaggccg aacagcagcg ccaccagggc ggcgcccagg cgcgccagcg
     2521 cgcgctggcg cgagacgggg ccggcatcgg cgaaatattc gatgcgaatg tggcggccac
     2581 gcgccaccgc ggccgaactg gcgaccattg ccagcacgat catgaggaag atcgacacct
     2641 cttcggtcca ggcgaaggag gcgtcggtga agtagcgcac cagcacgttg acgaaggtga
     2701 tcagcgccag cagcgccatg atgatcaccg tcagccagtc ttcgatggcc agcggcacgc
     2761 gggtggcggc gccggctggc gcggtggggt cggcgccctg gggcggcggg gaatcggcag
     2821 gcagggacat gaaggcagga cgcccggcgc gggcggcacg ccggcgcgcc ccgcgcgtcg
     2881 gcggatacaa atggtcgatg ttaaacgccg cggggtggcc tcaggcgccc cggacggcta
     2941 tcatcgtccc catgcaaacc ctgagcgatc gttgcttgcg cggccccgcg caaggcgggc
     3001 cggccccctg ggccggccgg cgttcgcctg cgcgcggcgc gcgcgtcggg gtgacgccat
     3061 cgtcggcgcg ggccccagcg gcctgccgtg cgtgcctgac ggccgcaccc cgctgagccc
     3121 gacgatcccc acgctatccc gctttaccga gcacagcgcc ccaccggatc gtcgggctgc
     3181 cccgcccggc gcggcttgcc gcgcctgccg gccagctgcc gcgcgccggc gctccctcat
//
LOCUS       DAOOOG010000232         3224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_36292_length_3224_cov_36.263806, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000232 DAOOOG010000000
VERSION     DAOOOG010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36292_length_3224_cov_36.263806"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PK242_12510"
     CDS             <1..857
                     /locus_tag="PK242_12510"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOZ94884.1"
                     /translation="FTVTCATDGNHGRSVAWGAQLLGCGCVIYLHATVSAGRQAAIEA
                     LGAQVVRTPGNYDDAVRQADADARRLGRHVISDTSYPGYMDVPRDVMQGYQLMVDEAV
                     QQLGQRPTHVFVQGGVGGLAAAVCGWFWERDGGARPRLVVVEPERADCLLRSARAGRP
                     VAVTGALDTLMAGLACGEVSLLAWDILASGADAFASIRDAAAVQAMRLLAAPTGSDAP
                     IVAGESAVAGLAAAIALAQVPAQRAALGIDARSRLLLFGSEGATDPALYAQLVGRTPQ
                     QVLAGQVR"
     gene            882..1643
                     /locus_tag="PK242_12515"
     CDS             882..1643
                     /locus_tag="PK242_12515"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HOZ94885.1"
                     /translation="MPPPGPRFDRLDALRALALLWMTAYHFAFDLNHFGHIRQDFYAD
                     PVWTAQRTAIVSLFLLCAGAGQALAVQAGQSAARFARRWAQVAGCALLVSLGSWWMFP
                     KSWISFGVLHGMALMLLLTRALLRRGALRGRWPWLGGLLLIAAAPLAGAWLRQAGPPA
                     LAQALDGRWLNGLGIVTRKPITEDYVPLLPWLGVMWLGVGAAQWAPLRAWLARPAGAA
                     LRALAPLGRWSLSYYMLHQPVLIGLLLAWAALRAG"
     gene            complement(1675..2520)
                     /locus_tag="PK242_12520"
     CDS             complement(1675..2520)
                     /locus_tag="PK242_12520"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HOZ94886.1"
                     /translation="MLLGALALGTRAWAGPADQAPALLLAQPWQPGTDLTDWWVSEKY
                     DGVRGFWDGRTLRTRGGERIAAPPWFTAGWPATPLDGELWAGRGGFEQVQSTVARQSP
                     DDAAWRRLRYMVFDLPAHPGTFDQRLPALRATVAAMDQPWVQAVPQRRVASQAQLQAL
                     LRQVVAGGGEGLMLHRGSSHYRAGRGDDLRKLKTHDDAEARVIAHLPGRGRHAGRLGA
                     LWVETPDGRRFALGAGFSDAERADPPPVGSWVTYRYRGLHAGSGLPRFASFLRVRADE
                     ARFRR"
     gene            complement(2608..>3224)
                     /locus_tag="PK242_12525"
     CDS             complement(2608..>3224)
                     /locus_tag="PK242_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ94887.1"
                     /translation="VFDCDVGSDEQIERMFRDLAQVWPRFDGFVHAIGFAPREAIAGN
                     FFDGLSRENFRVAHDISAYSFPGMAKAALPYLSDKAALLTLSYLGALRSIPNYNTMGL
                     AKASLEASVRYMAEALGGRGVRVNGISAGPIKTLAASGIKDFGKLLGYVAQAAPLRRN
                     VTIDDVGNVAAFLMSDLASGVTAEITYVDGGFSQTAGLSADMVG"
BASE COUNT          375 a         1170 c         1230 g          449 t
ORIGIN      
        1 agttcaccgt cacctgcgcc accgacggca accacgggcg ctcggtggcc tggggtgcgc
       61 agctgctggg ctgcggctgc gtgatctacc tccacgccac cgtcagcgcc gggcgccagg
      121 ccgccatcga agccttgggc gcccaggtgg tgcgcacacc cggcaactac gacgacgccg
      181 tgcgccaggc cgacgccgac gcgcggcggc tggggcgcca tgtgatttcc gacacctcct
      241 accccggcta catggatgtg ccgcgcgacg tcatgcaggg ctatcagctg atggtggacg
      301 aagccgtgca gcagctcggc cagcggccga cccacgtgtt cgtgcagggc ggcgtgggcg
      361 ggctggcggc ggccgtgtgc ggctggttct gggagcgcga cggtggtgcc cgcccgcgcc
      421 tggtggtggt cgaacccgaa cgggccgact gcctgctgcg cagcgcccgc gccggccggc
      481 cggtggcggt gacgggcgcg ctcgacacgc tgatggcggg cctggcctgc ggcgaagtgt
      541 cgctgctggc ctgggacatc ctggccagcg gcgccgacgc ctttgccagc atccgcgacg
      601 ccgccgccgt gcaggccatg cgcctgctgg ccgcgccgac ggggagcgac gcgcccatcg
      661 tcgccggcga atcggcggtg gccgggctgg ccgccgccat cgcgctggcg caggtgccgg
      721 cgcagcgcgc ggcgctgggc atcgacgccc gctcgcgcct gctgttgttc ggcagcgaag
      781 gcgccaccga cccggccctc tacgcgcagc tggtggggcg cacgccgcag caggtgctgg
      841 ccgggcaggt ccgctgaccc cgcgcatccc gcgcatcgcc catgccgccc cccggccccc
      901 gcttcgaccg cctggacgcg ctgcgcgcgc tggcgctgct gtggatgacg gcctaccact
      961 tcgcgttcga cctcaaccac ttcggccaca tccgccagga cttctatgca gacccggtgt
     1021 ggacggcgca gcgcacggcc atcgtcagcc tgttcctgct gtgcgccggc gccgggcagg
     1081 cgctggcggt gcaagccggc cagagcgcgg cccgctttgc gcgccgctgg gcccaggtgg
     1141 cgggctgcgc gctgctggtg tcgctggggt cgtggtggat gttccccaag agctggatca
     1201 gcttcggcgt gctgcacggc atggcgctga tgctgctgct gacgcgcgcg ctgctgcggc
     1261 gtggcgcgct gcgcggccgc tggccgtggc tgggcggcct gctgctgatc gccgccgcgc
     1321 cgctggccgg ggcctggctg cgccaggccg ggccgccggc gctggcgcag gcgctcgatg
     1381 gccgctggct gaacgggctg ggcatcgtca cgcgcaagcc gatcaccgag gactacgtgc
     1441 cgctgctgcc ctggctgggc gtgatgtggc tgggcgtggg cgcggcccag tgggcgccgc
     1501 tgcgcgcctg gctggcgcgc ccggccggcg ccgcgctgcg cgcgctggcg ccgctggggc
     1561 gctggagcct gagctactac atgctgcacc agccggtgct gatcgggctg ctgctggcct
     1621 gggcggcgct gcgggctggc tgacgcccgg cggtgcgcgc cggcgcggcc ccgctcagcg
     1681 gcgaaagcgc gcctcgtcgg cgcgcacgcg cagaaagctg gcaaagcgcg gcaggccgct
     1741 gcccgcatgc aggccgcggt agcggtacgt gacccagctg cccacgggcg gcgggtcggc
     1801 gcgctcggcg tcgctgaagc cggcgcccag cgcaaagcgc cggccatcgg gcgtttcgac
     1861 ccacagcgcg cccagccggc cggcgtggcg gccgcgcccg ggcaggtggg cgatgacgcg
     1921 cgcctcggcg tcgtcgtgcg tcttcagctt gcgcaggtcg tcgccgcgcc cggcgcggta
     1981 gtgcgagctg ccgcggtgca gcatcaggcc ttcgccgccg cccgccacca cctggcgcag
     2041 caacgcctgc aactgcgcct ggctggccac gcgccgttgc ggcacggcct gcacccaggg
     2101 ctggtccatg gcggcgacgg tggcgcgcag ggcgggcagg cgctgatcga aggtgccggg
     2161 gtgggccggc aggtcgaaca ccatgtagcg caggcggcgc caggccgcgt cgtcggggct
     2221 ctggcgcgcc acggtggact gcacctgctc gaagccgccg cgcccggccc acagctcgcc
     2281 gtccagcggc gtcgcgggcc agccggcggt gaaccagggc ggggcggcga tgcgctcgcc
     2341 gccgcgcgtg cgcagcgtgc ggccgtccca gaagccgcgc acgccgtcgt acttctcgct
     2401 cacccaccag tcggtcaggt cggtgccggg ctgccacggc tgggccagca gcagcgccgg
     2461 cgcctggtcg gccggaccgg cccatgcgcg cgtgcccagg gccagggcgc cgagcagcag
     2521 ggtgcgtcgc ttcacactat caaatcaata gctcttggcg ctcgccagac aagcgccaga
     2581 ggctgatttg gcttgcggcc gatgggctca gccgaccatg tcggccgaca gccctgcggt
     2641 ctggctgaag ccgccgtcga cgtaggtgat ttccgccgtc acgccgctgg ccaggtcgct
     2701 catcaggaag gcggcgacgt tgccgacgtc gtcgatggtg acgttgcggc gcagcggcgc
     2761 ggcctgcgcc acgtagccca gcagcttgcc gaagtccttg atgccgctgg ccgccagcgt
     2821 cttgatcggg ccggcgctga tgccgttgac gcgcacgccg cggccgccca gggcttcggc
     2881 catgtagcgc acgctggctt ccaggctggc cttggccagg cccatggtgt tgtagttggg
     2941 gatgctgcgc agcgcgccca ggtaggacag cgtcagcagc gccgccttgt cgctcaggta
     3001 gggcagggcg gccttggcca tgccggggaa gctgtaggca ctgatgtcgt gcgcgacgcg
     3061 gaagttttcg cgcgacagac cgtcgaagaa gttgccggca atcgcctcgc gcggcgcaaa
     3121 gccgatggcg tgcacgaagc cgtcgaacct cggccacacc tgggccaggt cgcggaacat
     3181 gcgctcgatc tgctcgtcgc tgccgacgtc gcagtcgaac acca
//
LOCUS       DAOOOG010000233         3224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_36293_length_3224_cov_26.997160, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000233 DAOOOG010000000
VERSION     DAOOOG010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36293_length_3224_cov_26.997160"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /gene="gatB"
                     /locus_tag="PK242_12530"
     CDS             complement(<1..406)
                     /gene="gatB"
                     /locus_tag="PK242_12530"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229956.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOZ94888.1"
                     /translation="MMSTLIQGYEVVIGFETHAQLRTRSKLFSRAATAFGAAPNTQAS
                     AVDLALPGTLPVMNREAVRCAIRLGLALGSRIAPRSVFDRKNYFYPDLPKGYQISQFE
                     QPVVQGGEVSFFVGDEKKTVRLVRAHLEEDAGK"
     gene            complement(420..1883)
                     /gene="gatA"
                     /locus_tag="PK242_12535"
     CDS             complement(420..1883)
                     /gene="gatA"
                     /locus_tag="PK242_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOZ94889.1"
                     /translation="MSALHDLTVAELASRLRGRQVSALELARHLLARSQAHAGLGAFL
                     AQDEAVTLAQARAADARLASGDAPLLTGVPVAHKDVFVTTDFPTTAGSRMLAGYRSPF
                     ESTVTARLAEAGMVCLGKLSCDEFAMGSSNENSAFGPVKNPWDPSRVPGGSSGGSAAA
                     VAARLTPAATGSDTGGSIRQPASFCGLTGIKPTYGRVSRHGMIAFASSFDQAGVMARS
                     AEDCALLLSAMSGADVDRDSTSLDLPAEDFTRGLHDSIDGLRIGIPQEFFGDGLAADV
                     RAALDGALAELCKLGARLVDIRLPRTELSIPVYYILTPAEASTNLSRFDGVRYGHRAP
                     EYADLEELYKKSRSQGFGEEVQRRIIIGTYVLSHGYYDAYYLQAQKLRRMIADDLQRA
                     FGECDVIAGPVSPGVAWPLGAHSADPLADYLADIYTLTASMAGLPAMSVPAGFGAGGM
                     PVGLQLIGNYLQEARLLNVAHRLQQATDWHTRKPQGY"
     gene            complement(1880..2179)
                     /gene="gatC"
                     /locus_tag="PK242_12540"
     CDS             complement(1880..2179)
                     /gene="gatC"
                     /locus_tag="PK242_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOZ94890.1"
                     /translation="MALTPDDISHIAHLARLELTTEQSERMLTQLNDFFAIAQAIQAE
                     DTTGVQPLAHPLAAIQDVQLRLRDDVVSEPDRRAAYQQGAPAVEDGLFLVPRVIE"
     gene            2380..>3224
                     /locus_tag="PK242_12545"
     CDS             2380..>3224
                     /locus_tag="PK242_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOZ94891.1"
                     /translation="MFGALRRYFSTDLAIDLGTANTLIYVRDKGIVLDEPSVVAIRHE
                     GGPQGKKTIQAVGSEAKAMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVHPR
                     GLFKPSPRIIICVPCGSTQVERRAIRESALGAGASEVYLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSARVGGDKFDDAIINYIRRNYGMLIGDPTAEA
                     IKKQIGSAFPGSEVREMEVKGRNLSEGVPRSFTISSNEILEALTEPLNNIVSEVKKAL
                     EETPP"
BASE COUNT          518 a         1093 c         1122 g          491 t
ORIGIN      
        1 acttgccggc gtcttcttcc aggtgggcgc gcaccaggcg cacggtcttt ttctcgtcgc
       61 cgacgaagaa cgacacctcg ccgccctgca ccaccggctg ctcgaactgg ctgatctggt
      121 agcccttggg caggtcgggg tagaagtagt tcttgcggtc gaacacgctg cgcggcgcaa
      181 tgcgcgagcc cagcgccagc ccgagccgga tggcgcagcg caccgcctcg cggttcatca
      241 ccggcagcgt gccgggcagc gccagatcga ccgcgctggc ctgggtgttg ggggcggcgc
      301 cgaacgccgt ggcggcgcgt gaaaacagct tgctgcgcgt gcgcagctgg gcgtgcgtct
      361 cgaagccgat gacgacttcg tagccctgga tcagtgtgct catgatgcgc ggcccctctt
      421 caataaccct ggggcttgcg ggtgtgccag tcggtggcct gctgcaggcg gtgcgccacg
      481 ttcagcagac gcgcttcctg caggtagttg ccgatcagct gcagaccgac cggcatgccc
      541 ccggcgccaa agccggccgg cacgctcatg gccggcagac cggccatgga ggcggtcagg
      601 gtgtagatgt cggccaggta gtcggccagc gggtcggcgc tgtgcgcgcc cagcggccag
      661 gccacgccgg gcgacaccgg gccggcgatg acgtcgcatt cgccgaacgc gcgctgcagg
      721 tcgtcggcga tcatgcggcg caacttctgc gcctgcaggt agtaggcgtc gtagtagccg
      781 tgcgacagca cgtaggtgcc gatgatgatg cgccgctgca cttcttcgcc aaagccctgg
      841 ctgcggctct tcttgtacag ctcctccagg tcggcgtatt cgggcgcgcg gtggccgtag
      901 cgcacgccgt cgaagcggct caggttggtg ctggcctcgg ccggggtgag gatgtagtag
      961 accgggatcg acagctcggt gcgtggcagg cggatgtcca ccagccgggc gcccagcttg
     1021 cacagctcgg ccagcgcgcc gtccagcgcc gcgcgcacgt cggccgccag gccatcgccg
     1081 aagaactcct gcgggatgcc gatgcgcagg ccgtcgatcg agtcgtgcag gccgcgcgtg
     1141 aagtcctcgg ccggcaaatc gagcgaggtc gagtcgcgat cgacatcggc gccgctcatg
     1201 gccgagagca gcagcgcgca gtcctcggcg ctgcgcgcca tcacgcccgc ctggtcgaag
     1261 ctggaggcaa aggcaatcat gccgtggcgc gacacgcgcc cgtaggtcgg cttgatgccc
     1321 gtgaggccgc agaagctggc cggctggcgg atcgagccgc cggtgtccga gccggtggcc
     1381 gccggcgtca ggcgcgcggc cacggcggcg gcgctgccgc ccgaggagcc gcccggcacg
     1441 cgcgatggat cccaggggtt cttgaccggt ccgaaggccg agttctcgtt ggatgagccc
     1501 atggcgaact cgtcgcaact gagcttgccc aggcacacca tgccggcctc ggccagcctg
     1561 gccgtcaccg tgctctcgaa cggcgagcgg tagccggcca gcatgcgcga gccggcggtg
     1621 gtgggaaagt cggtggtgac gaacacgtcc ttgtgcgcca ccggcacgcc cgtcagcagc
     1681 ggcgcatcgc cgcttgccag ccgggcatcg gcggcgcgcg cctgcgccag cgtcaccgcc
     1741 tcgtcctgcg ccagaaaggc gcccagcccg gcatgggcct ggctgcgcgc cagcaggtgc
     1801 cgcgccagct ccagcgccga cacctgccgt ccacgcaggc ggctggccag ctcggccacg
     1861 gtgagatcat gcagcgcgct cattcgatca ccctcggcac caggaacagg ccgtcctcga
     1921 ccgccggcgc gccctgctgg taggcggcgc ggcggtcggg ctcgctcacc acgtcgtcgc
     1981 gcagacgcag ctgcacgtcc tggatggcgg ccagcgggtg cgccagcggc tgcacgccgg
     2041 tggtgtcctc ggcctgtatc gcctgggcga tggcaaagaa atcgttcaat tgcgtgagca
     2101 tgcgctcact ttgttcggta gtcagctcaa gtcgcgccag gtgcgcgata tggctgatgt
     2161 cgtcaggtgt cagggccatg aaaaaggcac ggaatgtcgg ggatcgatgg ggtgttgcgc
     2221 cgccgtcgcc gcagggacgc cgggcggcga tgcgttatta tccgaccttt ggcgcaacgg
     2281 ccccgaattg gccgatattg gcgccaaatc acgctcattt cgcattgaat caacggttgc
     2341 cggcgtctgg tgctggccgg cagcccagaa gggtatttca tgtttggagc tctccgtcgg
     2401 tacttttcca ccgacctggc catcgacctg ggcaccgcca acaccctgat ttacgtccgc
     2461 gacaagggca tcgtgctcga cgagccctcg gtggtcgcca tccgccacga aggcggcccg
     2521 cagggcaaga agaccatcca ggcggtgggc agcgaggcca aggccatgct gggcaaggtg
     2581 ccgggcaaca tcgaggccat ccgccccatg aaggacggcg tgatcgccga cttcaccgtc
     2641 accgagcaga tgctcaagca gttcatcaag atggtgcacc cgcgcggcct gttcaagccc
     2701 agcccgcgca tcatcatctg cgtgccctgc ggctccaccc aggtcgagcg ccgcgccatc
     2761 cgcgaatccg ccctgggcgc cggcgccagt gaggtctacc tgatcgagga gcccatggcc
     2821 gccgccatcg gcgccggcct gccggtgtcc gaggccagcg gctcgatggt ggtggacatc
     2881 ggcggcggca ccaccgaagt gggcgtcatc agcctgggcg gcatggtcta caagggcagc
     2941 gcgcgcgtgg gcggcgacaa gttcgacgac gccatcatca actacatccg ccgcaactac
     3001 ggcatgctga tcggcgaccc gacggccgag gcgatcaaga agcagatcgg cagcgccttc
     3061 cccggcagcg aggtgcgcga gatggaagtg aaggggcgca atctgtccga aggcgtgccg
     3121 cgcagcttca ccatcagcag caacgaaatc ctcgaagcgc tcaccgagcc gctcaacaac
     3181 atcgtctccg aggtcaagaa ggcgctggag gaaaccccgc ccga
//
LOCUS       DAOOOG010000234         3178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_37010_length_3178_cov_31.765290, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000234 DAOOOG010000000
VERSION     DAOOOG010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37010_length_3178_cov_31.765290"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1319
                     /gene="prpF"
                     /locus_tag="PK242_12550"
     CDS             105..1319
                     /gene="prpF"
                     /locus_tag="PK242_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HOZ94892.1"
                     /translation="MHPPDACLPPLRIAASYLRGGTSKGVFFCLDDLPAAARQPGPVR
                     DALLRRVLGSPDPYGQQIDGMGGATSSTSKAVILAPASRPDHDVDYLFAQIGVDSGLV
                     DWSGNCGNLSAAVGPFAIARGLLPPARVPRDGVCSVRIWQANIGKTIVAQVPMAGGQV
                     QEAGDFELDGVAFAAAEIGLSFLDPAEGGAGAGGLFPTGRLVDELDVPGIGRLPATLI
                     NAGIPTVFVNARDVGCSGTERQADLNGDAQALARFEAIRAQGALKMGLIATLTEAAQR
                     QHTPKIAFVAPPADYRAASGRQVRAADIDLLVRALSMGRLHHAMMGTAAVAIATAAAI
                     AGTLVHAAAGGGARPTVRFGHPSGTLRVGAQAVQSATGDWQVREVRMSRSARVLMEGW
                     VRVPAEVLGAGA"
     gene            1457..2434
                     /locus_tag="PK242_12555"
     CDS             1457..2434
                     /locus_tag="PK242_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ94893.1"
                     /translation="MTCHRRTLLASAALGALSAALPAAAQDTWPTKTVRLIVPYAAGG
                     FADTRARKIADALGKTLGQPVIVDNRAGAGGVTGTDAIAKATDGHTFGFGSPAPLVTN
                     PMLMKKMPYDAEKAFKPIILIETAPLFLSTAPNQNFKSVKELLDFARANPGKLTYGSS
                     GVGGAHHLSAELLAYQTKTETTHVPYKGGALAANDLMGGHLAFMFEMGYSALPAIQAG
                     KIKALAVSSRQRVAVLPDVPTLDEAGVKGFESYNWLGMIAPASTPDAVIARLNEAVNQ
                     ALKQPDLRQMIEGSAGIVVGGTPAAYGQFLAQERAKWAPVIKAANISLD"
     gene            2456..>3178
                     /locus_tag="PK242_12560"
     CDS             2456..>3178
                     /locus_tag="PK242_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HOZ94894.1"
                     /translation="MNTRQRLRQLAAARRGALVPGAFNALSARVIEDLGFEAVYITGA
                     GVTNMWLGLPDQGFMGLAEIADHTARIRDAVELPLLVDADTGFGNALNVYHTVRTLER
                     AGADGIQLEDQVAPKRCGHFDGKAVISTEEAVSKIKAAVDARRDAELVVMARTDAAAT
                     QGFEAALERAQRFAEAGADILFVEAVTRAEEIRALPQRLPTPQLMNMVIGGKTPIVDA
                     AELGRLGYGLVLYANAALQGALA"
BASE COUNT          484 a         1176 c         1083 g          435 t
ORIGIN      
        1 gcgcatgaac gcactggccg acgacctggg gctgtaaccc tcaaaacggc cgtcggccgc
       61 ttgccatcgt cacgtgcgcc gcgcttggcg cgtaccatgg cgccatgcac ccgcccgacg
      121 cctgcctgcc cccgcttcgg attgccgcca gctacctgcg cggcggcacc agcaagggcg
      181 tgttcttctg cctggatgat ctgcccgccg cggcgcgcca gcccggtccg gtgcgcgacg
      241 cgctgctgcg gcgcgtgctg ggcagtcccg acccctacgg gcagcagatc gacggcatgg
      301 gcggcgccac gtcgtccacc tccaaggccg tgatcctggc cccggccagc cggcccgacc
      361 acgacgtcga ctacctgttt gcccagatcg gcgtcgacag cgggctggtg gactggtcgg
      421 gcaactgcgg caacctgtcg gccgccgtgg ggccgttcgc catcgcccgc ggtctgctgc
      481 cgcccgcgcg cgtgccgcgc gacggcgtct gcagcgtgcg catctggcag gccaacatcg
      541 gcaagaccat cgtcgcccag gtgcccatgg ccggcggcca ggtgcaggaa gcgggcgact
      601 tcgagctcga cggcgtggcc ttcgcggccg ccgagatcgg gctgagcttt ctcgacccgg
      661 ccgagggcgg cgcaggcgcg ggtggactgt ttcccaccgg ccgcctggtc gacgagctgg
      721 acgtgcccgg catcggtcgc ctgccggcca cgctgatcaa cgccggcatc cccaccgtgt
      781 tcgtcaacgc ccgcgatgtc ggctgcagcg gcaccgagcg acaggccgac ctcaacggcg
      841 acgcgcaggc gctggcgcgc ttcgaggcca tccgcgccca gggtgccctg aagatgggcc
      901 ttatcgccac gctgaccgaa gccgcccagc gccagcacac gcccaagatc gccttcgtgg
      961 cgccgccggc cgactaccgc gccgccagtg gccgacaggt gcgcgcggcc gacatcgacc
     1021 tgctggtgcg cgcgctgtcg atgggccggc tgcaccacgc catgatgggc acggcggcgg
     1081 tggccatcgc caccgccgcc gccattgcgg gcaccctggt gcatgccgcc gccggtggcg
     1141 gcgcgcgccc gacggtgcgc ttcggtcacc cctccggcac gctgcgcgta ggcgcgcagg
     1201 cggtgcaaag cgcgaccggc gactggcagg tgcgcgaagt gcgcatgagc cgcagcgccc
     1261 gcgtgctgat ggaaggctgg gtgcgcgtgc cggccgaggt gctgggcgcc ggggcctgac
     1321 gcgccgctgt gttgcaagaa aaagatgctt gcagcgattt accagtctgc gccagcagct
     1381 attgaatttg aagcgccatg gaaataggcc ggtcccggcg gcccataatg gtcttttcga
     1441 tccgaaggac gcctgcatga cctgccaccg ccgcaccctg cttgccagcg ccgccctggg
     1501 cgcgctgtct gccgccctgc ctgccgccgc gcaggacacc tggcccacca agaccgtgcg
     1561 cctgatcgtg ccctacgccg ccggcggctt tgccgacacg cgcgcgcgca agatcgccga
     1621 cgcgctgggc aagaccctgg gccagccggt catcgtcgac aaccgggcgg gtgccggcgg
     1681 cgtcaccggc accgatgcca tcgccaaggc caccgacggc cacaccttcg gcttcggctc
     1741 gcccgcgccg ctggtgacca acccgatgct gatgaagaag atgccctacg acgccgaaaa
     1801 ggcgttcaag cccatcatcc tgatcgagac cgcgccgctg ttcctgtcca ccgcgccgaa
     1861 ccagaacttc aagtcggtca aggaactgct ggacttcgcc cgcgccaacc ccggcaagct
     1921 gacctacggc tcctccggcg tgggtggcgc ccaccatctg tcggccgagt tgctggccta
     1981 ccagaccaag accgaaacca cccatgtccc ctacaagggc ggcgcgctgg ccgccaacga
     2041 cctgatgggt ggccacctgg ccttcatgtt cgagatgggc tactcggccc tgccggccat
     2101 ccaggccggc aagatcaagg cgctggccgt ctccagccgc cagcgcgtgg ccgtgctgcc
     2161 cgacgtgccg acgctggacg aagccggcgt caagggcttc gagtcctaca actggctggg
     2221 catgatcgcg ccggccagca cgcccgatgc cgtcatcgcg cggctgaacg aagccgtcaa
     2281 ccaggcgctc aagcagcccg atctgcgcca gatgatcgag ggcagcgccg gcatcgtcgt
     2341 cggcggcacg ccggcggcct atggccagtt cctggcgcag gagcgcgcca agtgggcgcc
     2401 ggtcatcaag gccgccaaca tctcgctcga ctgatccaga aagaaggcac ctggcgtgaa
     2461 cacccgacaa cgactcagac aactggccgc ggcgcggcgc ggcgcgctgg tgccgggcgc
     2521 cttcaacgcg ctgtcggcgc gcgtgatcga ggacctgggc ttcgaggccg tctacatcac
     2581 cggcgccggc gtcaccaaca tgtggctggg cctgcccgac cagggcttca tgggcctggc
     2641 cgagatcgcc gaccacacgg cgcgcatccg cgacgcggtg gagctgccgc tgctggtgga
     2701 tgccgacacc ggcttcggca acgcgctgaa cgtctaccac acggtgcgca cgctggagcg
     2761 cgccggcgcc gacggcatcc agctggaaga ccaggtggcg cccaagcgct gcgggcattt
     2821 cgacggcaag gcggtcatca gcaccgagga agcggtcagc aagatcaagg ctgccgtcga
     2881 tgcccggcgc gacgccgagc tggtggtcat ggcgcgcacc gacgccgccg ccacccaggg
     2941 tttcgaggcg gcgctggagc gcgcgcagcg ctttgccgag gccggcgccg acatcctgtt
     3001 cgtcgaagcc gtcacccgcg ccgaagaaat ccgcgccctg ccacagcgcc tgccgacgcc
     3061 gcagctgatg aacatggtga tcggcggcaa gacgccgatc gtcgacgccg ccgagctggg
     3121 gcggctcggc tacggcctgg tgctgtacgc caacgcggcg ctgcagggcg cgctggcc
//
LOCUS       DAOOOG010000235         3174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_37074_length_3174_cov_30.890349, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000235 DAOOOG010000000
VERSION     DAOOOG010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37074_length_3174_cov_30.890349"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3174
                     /gene="smc"
                     /locus_tag="PK242_12565"
     CDS             <1..>3174
                     /gene="smc"
                     /locus_tag="PK242_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOZ94895.1"
                     /translation="LNSIKLSGFKSFAEPTNFMLPGQLVGVVGPNGCGKSNIMDAVRW
                     VLGESKASELRGESMQDVIFNGTTTRKPASRSSVELVFDNADHRAGGQWAQFAEISVK
                     RVLTRDGTSSYYINNQPVRRRDVQDVFLGTGLGPRAYAIIGQGTISRIIESRPEELRL
                     FLEEAAGVSKYKERRRETENRLHDTRENLTRVEDILRELNHNLDKLEKQAEVAQQYQN
                     LQAAAQLKQNQLWFLKRAEADEGQGRLRQEADKAVNDLESRLADLRHVEAELETIRQA
                     HYAAGDQVNQAQGLLYEASAEVGRLEAEIRFVVEGRQRAEARLLQLKEQFGQWAERRS
                     QAEADIEQLAVQGVDAEDRAITLSAQLEEQQFTLPDLQEALRAAQAGAAEQRTAVAQV
                     QQQIQVLAAEQRGIEEQTRQLQARRERLAADRNALAAPDEQRLLNLQQQLSAAQDGAE
                     VAQARLAELQDSVPRLDDERRAAQQAVNTEAARQADLSARLDALRALQDKLKTDGKLQ
                     PWLHKHGLDGLPGLWSRLHVEPGWEAALEAALRERMSALEVSRLDMVRAFAQDAPPAK
                     LAFYSPPAAALPEGAAGLPRLADLLRLHDAGQKALLADWLAGCYTADSLEAALAQRAQ
                     LKPGEAIYVASGHAVGAHGVSFYAPDSEQAGLLARAQEIENLDKQLRAQQLISEEARS
                     ALVRAEAAYSAAAEQLTGSRRDAAEAQQRAHELQVETLRLSQLAEQTRARSEQIGTDL
                     AEVDAGLEALQERRITAEARFEELDMQLADTQERHAGLDQRVIDAEQRLNQAREQQRT
                     LERELQEAQFAQRSLQARRDELTRAIDTAQQQGAAIEAEQQRASEELERLTDAAAQAG
                     LQAALGLKLEREQALAAQRSSYDDLTARLRASDERRLRLERELDPLRQRITDLQLKEQ
                     AARLGTEQYTQLLADAQADLQAVARSIEEGSVRLNGLQGEIDRLHQGIQALGAVNLAA
                     LDELTTARERKQFLDAQSADLTEAMTTLEDAIKKIDAETRELLGGTFNTVNEHFGRMF
                     PELFGGGQAKLVMTGEEILDSGVQVMA"
BASE COUNT          578 a         1133 c         1113 g          350 t
ORIGIN      
        1 tctcaattcc atcaagctgt cgggcttcaa gtccttcgcc gaacccacca acttcatgct
       61 gcccggccaa ctggtcggcg tggtgggccc caatggctgc ggcaaatcca acatcatgga
      121 cgcggtgcgc tgggtgctgg gcgaatccaa ggcgtccgag ctgcgcggcg agtcgatgca
      181 ggacgtcatc ttcaacggca ccaccacgcg caaaccggcg tcgcgctcgt ccgtggagct
      241 ggtgtttgac aacgccgacc accgcgccgg cggccagtgg gcgcagtttg ccgagatttc
      301 ggtcaagcgc gtgctgacgc gcgacggcac cagcagctac tacatcaaca accagccggt
      361 gcgccggcgc gacgtgcagg acgtgtttct gggcaccggt ctgggcccgc gcgcctacgc
      421 catcatcggc cagggcacga tcagccgcat catcgagtcg cgacccgaag agctgcgcct
      481 gtttctggag gaggccgcgg gcgtttccaa gtacaaggag cgccggcgcg aaaccgaaaa
      541 ccgtctgcac gacacgcgcg agaacctcac gcgggtggaa gacatcctgc gcgaactcaa
      601 ccacaacctc gacaagctgg aaaagcaggc cgaggtggcg cagcagtacc agaacctgca
      661 ggcggcggcc cagctcaaac agaaccagct gtggtttctc aagcgcgccg aggccgacga
      721 gggccagggc cggctcaggc aggaggccga caaggcggtc aacgacctgg agtcgcgcct
      781 ggccgatctg cgccatgtcg aagccgagct ggaaaccatc cgccaggcgc attacgccgc
      841 cggcgatcag gtcaaccagg cgcagggcct gctgtacgaa gccagcgccg aggtcggccg
      901 gctggaggcc gagatccgct tcgtcgtcga aggccgccag cgcgccgaag cgcgcctgct
      961 gcaactgaag gagcaattcg gccagtgggc cgagcggcgc agccaggccg aggccgacat
     1021 cgagcaactg gcggtccagg gcgtcgacgc cgaagaccgt gccatcacgc tctcggcgca
     1081 actggaggag cagcagttca cgctgcccga cctgcaggag gcgctgcgtg ccgcccaggc
     1141 cggcgccgcc gagcagcgca ccgccgtcgc ccaggtgcag cagcagatcc aggtgctggc
     1201 cgccgagcag cgcggcatcg aagagcagac gcgccagctg caggcgcggc gcgagcgcct
     1261 ggcggccgac cgcaacgccc tggccgcgcc cgacgagcag cgcctgctga acctgcagca
     1321 gcagctgtcg gcggcgcagg acggcgccga agtcgcccag gcgcggctgg ccgagctgca
     1381 ggacagcgtg ccgcggctcg acgacgaacg ccgggcggcc cagcaggcgg tcaacaccga
     1441 agcggcgcgc caggccgatc tgtcggcgcg gctggacgcg ctgcgcgcgc tgcaggacaa
     1501 gctcaagacc gacggcaagc tgcaaccctg gctgcacaag cacgggctcg acggtctgcc
     1561 ggggctgtgg agccgcctgc acgtcgagcc gggctgggaa gcggcgctgg aagcggcgct
     1621 gcgcgaacgc atgtcggcgc tcgaagtcag ccgcctggac atggtgcgcg ccttcgccca
     1681 ggacgcgccg ccggccaagc tggccttcta cagtccgccg gccgccgcgc tgcccgaagg
     1741 cgcggccggc ctgccgcgtc tggccgacct gctgcgtctg cacgacgccg ggcagaaagc
     1801 cctgctggcc gactggctgg ccggctgcta caccgccgac agcctggaag ccgcgctggc
     1861 ccagcgcgct cagctcaagc cgggcgaggc catctatgtg gccagtggcc acgccgtcgg
     1921 cgcgcatggc gtcagcttct acgcgcccga ttccgaacag gccggcctgc tggcgcgcgc
     1981 gcaggaaatc gagaacctcg acaaacagct gcgcgcccag cagctcatca gcgaagaagc
     2041 gcgcagcgcc ctggtgcggg ccgaagccgc ttacagcgcc gccgccgaac agctgaccgg
     2101 cagccgccgc gacgccgccg aggcgcagca acgcgcgcac gagctgcagg tcgaaaccct
     2161 gcgcctgagc cagctggccg agcagacgcg cgcccgcagc gagcagatcg gcaccgatct
     2221 ggccgaggtc gatgccggcc tggaagcgct gcaggagcgc cgcatcaccg ccgaggcgcg
     2281 ctttgaagag ctggacatgc agctggccga cacgcaggag cgccacgccg gcctggacca
     2341 gcgcgtgatc gacgccgagc agcgcctgaa ccaggcgcgc gagcagcagc gcacgctgga
     2401 gcgcgagctg caggaggccc agttcgccca gcgcagcctg caggcgcgcc gcgacgagct
     2461 cacgcgcgcc atcgacaccg cccagcagca gggcgccgcc atcgaggccg agcagcagcg
     2521 cgccagcgag gaactggagc gcctgaccga cgccgccgcc caggccggcc tgcaggccgc
     2581 gctgggcctg aaactcgagc gcgaacaggc gctggcggcg cagcgcagca gctacgacga
     2641 cctgaccgcg cgcctgcgcg ccagcgacga acgccgtctg cggctggaac gcgagctcga
     2701 cccgctgcgc cagcgcatca ccgacttgca gctcaaggag caggcggcgc gcctgggcac
     2761 cgagcagtac acgcagctgc tggccgacgc ccaggccgac ctgcaggcgg tggcgcgctc
     2821 gatcgaggaa ggcagcgtgc gcctcaatgg cctgcagggc gaaatcgacc gcctgcacca
     2881 gggcatccag gcgctgggcg cagtcaacct ggcggcgctg gacgagctga ccacggcgcg
     2941 cgagcgcaag cagttcctgg acgcgcagtc ggccgacctg accgaggcca tgaccacgct
     3001 ggaagacgcc atcaagaaga tcgacgccga aacgcgcgag ctgctgggcg gcaccttcaa
     3061 caccgtcaac gagcacttcg gtcgcatgtt ccccgagctg ttcggcggcg gccaggcgaa
     3121 gctggtgatg accggcgagg aaatcctcga ctccggcgtg caggtgatgg cgca
//
LOCUS       DAOOOG010000236         3152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_37404_length_3152_cov_23.226348, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000236 DAOOOG010000000
VERSION     DAOOOG010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37404_length_3152_cov_23.226348"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1435
                     /locus_tag="PK242_12570"
     CDS             113..1435
                     /locus_tag="PK242_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOZ94896.1"
                     /translation="MSTLTIGLLLFVAMLLLMAIRVPIAVAMFVPGAVGYVILSGWDP
                     LLAHLRGAVYGRVSVYDLSVIPLFMLMGGFAVNGGLSKALFDFANALLGRFKGGMAMA
                     GILACAAFGAISGSTVATTATIAQVAYPEMRRISYSGRLATAALATGGTMGVLLPPSV
                     TLMVYAILTEQNITKTFLAAYVPAMLAALGYLVVIAIYVRLHPTHAPEARAASASEVA
                     QSAAGVWPIALIFVAVFGGIYGGIFTPTEGAAIGATLTFAITLINRQLTLKKAIESIR
                     TTAVTSGMIFMIFLGADMMNASLALSQLPNQLAELIAHAAIPPVLVMAVIMLFYVALG
                     CVMDEMSMILLTVPVVFPVIMGMDFFGLDASDKALWFGILILTVCAIGMIFPPVGLNV
                     YVMNGLAKDVPMVETYKGVFIFLAWDFIRLGLLIMFPGITLWLVRLLT"
     gene            1468..2505
                     /locus_tag="PK242_12575"
     CDS             1468..2505
                     /locus_tag="PK242_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOZ94897.1"
                     /translation="MSSKLNKLMAAAALSLLAGTAAQAQTVLKVHHFLPSGSSSQVKI
                     IAPWCAKIEKESGGELKCQIYPAMQLGGTPPQLLDQVRDGVADIVWSVPTYSAGRYTK
                     SEVFELPFMAINAKQGSQALWEYVQKNALDEYKGIKPLFMHTTEGYVLHSNKPILKLE
                     DMKGKKIRTATRISARMMSAMGGTPVQMPLPQVPDALSKGVVDGVLVPWEALPATKLH
                     EIVKYHLDAPVGSPRFANTIFIFGMNQAKYDSLSPKLKKVIDDNSGLATSAWAGEEGF
                     DNIVAPYSKLARDRGNTVSVIEPNELARWEKVTADVDEIWLKDATTKGYDGKKLIEEA
                     KALLKKYAPAK"
     gene            complement(2648..>3152)
                     /locus_tag="PK242_12580"
     CDS             complement(2648..>3152)
                     /locus_tag="PK242_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ94898.1"
                     /translation="VSDAEGHDLPVGEVGEVLIRGDSVMAGYWGNPEATAAALRDGWL
                     FTGDMGALDADGYLTLKDRSKDLIISGGSNIYPREVEEALLTAPGVAEVAVVGEPDPQ
                     WGENVVAFVVAQPGNEPSQETLDTHCLARIARFKRPKRYVFVPELPKNNYGKVLKTEL
                     RRWLASA"
BASE COUNT          505 a         1052 c         1015 g          580 t
ORIGIN      
        1 tctggattcc ctacatgctg atggtgcccg gcctggtgct gtgcaccctg gtcggtgtgt
       61 accaggcggt catctgctgg acgcagccga ccgcggccga aggagaggcc gcgtgagcac
      121 cttgaccatc ggactgctgc tgttcgtcgc catgctgttg ctgatggcga tccgtgtgcc
      181 gatcgccgtg gccatgttcg tgcccggcgc cgtgggatac gtgatcctgt ccggctggga
      241 cccgctgctg gcacacctgc gcggcgccgt gtatggccgc gtgtcggtgt acgacctgtc
      301 ggtgattccg ctgttcatgc tgatgggggg cttcgccgtc aacggcgggc tgtccaaggc
      361 gttgttcgac ttcgccaacg cgctgctggg ccgcttcaag ggcggcatgg cgatggcagg
      421 catcctggcg tgcgcggcct ttggcgccat ttccggttcc accgtggcca ccaccgccac
      481 catcgcccag gtggcctatc cggagatgcg ccgcatcagt tattcagggc gcctggccac
      541 ggcggcgctg gccaccggcg gcaccatggg cgtgctgctg ccgccttcgg tgacgctgat
      601 ggtgtacgcc atcctgaccg agcagaacat caccaagacc ttcctggccg cctacgtgcc
      661 ggccatgctg gcggcgctgg gttacctggt ggtgatcgcc atctacgtgc gcctgcaccc
      721 cacccacgcg cccgaggcgc gcgccgccag cgcgtccgag gtggcgcagt cggccgccgg
      781 cgtgtggccg atcgcgctga tcttcgtcgc cgtgttcggc ggcatctatg gcggcatctt
      841 cacgcccacc gagggcgcgg ccatcggcgc cacgctgacc ttcgccatca cgctgatcaa
      901 ccgccagctg acgctgaaga aggccatcga gtcgatccgc accacggccg tcacctcggg
      961 catgatcttc atgatcttcc tgggtgccga catgatgaac gcctcgctgg cgctgtcgca
     1021 gctgcccaac cagctggccg agctgatcgc ccacgccgcc attccaccgg tgctggtgat
     1081 ggccgtgatc atgctgttct acgtggcgct gggctgcgtc atggacgaaa tgtcgatgat
     1141 cctgctgacg gtgccggtgg tgttcccggt catcatgggc atggacttct tcggtctcga
     1201 cgcgtcggac aaggcgctgt ggttcgggat actcattctt accgtttgcg ccatcggcat
     1261 gatcttcccg ccggtcggtc tgaacgttta tgtgatgaac ggcctggcca aggatgtgcc
     1321 gatggtggag acctacaagg gcgtgttcat cttcctggcc tgggacttca tccgtctggg
     1381 cctgctgatc atgttccccg gcatcactct gtggctggtg cgcctgctga cctgaaagac
     1441 aacccttttt aaccaggaga taccttgatg agctcaaagc tgaacaaact gatggcggcc
     1501 gcggcgctgt cgctgctggc gggcaccgcc gcccaggcgc agaccgtgct gaaggtgcac
     1561 cacttcctgc cttcgggctc cagctcgcag gtcaagatca tcgcgccctg gtgcgccaag
     1621 atcgaaaagg aatcgggtgg cgagctcaag tgccagatct accccgccat gcagctgggc
     1681 ggcacgccgc cgcaactgct ggaccaggtg cgcgacggcg tggccgacat cgtgtggtcg
     1741 gtgcccacct actcggcggg ccgctacacc aagtccgaag tgttcgagct gcccttcatg
     1801 gccatcaacg ccaagcaggg ctcgcaggcg ctgtgggagt acgtgcagaa gaacgcgctg
     1861 gacgagtaca agggcatcaa gccgctgttc atgcacacca ccgaaggtta cgtgctgcac
     1921 tcgaacaagc cgatcctcaa gctggaggac atgaagggca agaagatccg caccgccacc
     1981 cgcatctcgg cccgcatgat gtcggccatg ggcggcacgc cggtgcagat gccgctgccg
     2041 caggtgccgg acgcgctgtc caagggcgtg gtcgacggcg tgctggtgcc ctgggaagcg
     2101 ctgcccgcca ccaagctgca cgagatcgtc aagtaccacc tcgatgcgcc ggtcggctcg
     2161 ccgcgctttg ccaacaccat cttcatcttc ggcatgaacc aggccaagta cgacagcctg
     2221 tcgcccaagc tgaagaaggt gatcgacgac aattcgggcc tggccacctc ggcctgggcg
     2281 ggcgaagagg gtttcgacaa catcgtcgcg ccgtactcca agctggcgcg cgaccgcggc
     2341 aacaccgtct ccgtcatcga gcccaacgag ctggcgcgct gggagaaggt cacagccgac
     2401 gtcgacgaga tctggctgaa ggacgccacg accaagggtt acgacggcaa gaagctgatc
     2461 gaggaagcca aggcgctgct gaagaaatac gcgccggcaa aataaggccg acgaccgccg
     2521 ccaagccgct gcctgcagcg gctcttttca tggtcattcg tgcagttggc gtccgtctgg
     2581 caagcgccaa aagctatttt aattatagcg tggtgatcgg tccggcggtg ccacctggca
     2641 cctcgcctca ggcgctggcc agccaccggc gcagctcggt cttcagcacc ttgccgtagt
     2701 tgttcttcgg cagctcgggc acgaagacgt agcgcttggg ccgcttgaag cgggcgatgc
     2761 gcgccaggca gtgggtgtcc agcgtctcct gcgacggctc gttgcccggt tgcgccacca
     2821 cgaaggccac cacgttctcg ccccattgcg ggtcgggctc gcccaccacc gccacctcgg
     2881 ccacgccggg ggcggtgagc agggcctcct cgacctcgcg cgggtagatg ttgctgccgc
     2941 cgctgatgat caggtccttg ctgcggtcct tcagcgtcag gtagccgtct gcgtccagcg
     3001 cgcccatgtc gccggtgaac agccagccgt cgcggagggc ggcggccgtg gcctcggggt
     3061 tgccccagta gccggccatc acgctgtcgc cccggatcag cacctcgccc acttcgccca
     3121 ccggcaggtc atgcccctcg gcgtcgctca cg
//
LOCUS       DAOOOG010000237         3147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_37489_length_3147_cov_25.441462, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000237 DAOOOG010000000
VERSION     DAOOOG010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37489_length_3147_cov_25.441462"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PK242_12585"
     CDS             <1..429
                     /locus_tag="PK242_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOZ94899.1"
                     /translation="TEVFAYGRMPLAFSARCFTARHYNLPKDDCGFACIQHPDGQLLK
                     TREGEAFLVLNGIQTQSARVYNLIGDLPELRALGVDVLRLSPQSQHMADIVAAFDAAR
                     RADTPDPDALARLRPMMPDEPCNGYWHGRSGMDLIEPALA"
     gene            458..979
                     /locus_tag="PK242_12590"
     CDS             458..979
                     /locus_tag="PK242_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOZ94900.1"
                     /translation="MTTPPLPTPFTLPRPVGALLSCLPAYPGSVLLVSAINLALARQL
                     PEDVTALLKDKRLSIRVRDARLGFDFTWNGRRFAPSAPHAEPDLAISANAQDFLLLAQ
                     RRQDPDTLFFNRRLVMEGDTELGLVVKNALDALELPVLDPAAWSPRAVLARWAPGLAE
                     RLPPFPPFSAPRR"
     gene            976..1371
                     /locus_tag="PK242_12595"
     CDS             976..1371
                     /locus_tag="PK242_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOZ94901.1"
                     /translation="MNPSGTHDHAPLVYSCSGCSSAAQLANHVALQLDRRGVAEMSCI
                     AGVGGDVPKLVRLATSGRPIVALDGCPLACVRSSLARHGVAPARHYQLHQYGVKKRTH
                     QDFDPAQAAEVLARIEADLRQHPLAPAHD"
     gene            1364..1948
                     /locus_tag="PK242_12600"
     CDS             1364..1948
                     /locus_tag="PK242_12600"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOZ94902.1"
                     /translation="MTELRRVIVGITGASGAIYGVRLLQVLRELASVTTHLVVSDAGW
                     RSLRYELDLAPAQVQALADEAHDIRNIGACIASGSFVTLGMVVAPCSMRTLAAIAHGL
                     GDNLLTRAADVVLKERRRLVLLARESPLHLGHLRNMVAVTELGAIVSPPVPAFYKRPR
                     SLEDIVDHSVARALDALGIEHKLSTRWPGLPTDY"
     gene            2159..>3147
                     /locus_tag="PK242_12605"
     CDS             2159..>3147
                     /locus_tag="PK242_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOZ94903.1"
                     /translation="MPYRDLRDFMAQLEAAGELRRIAEPVSPHLEMTALADRVLRAGG
                     PALLFENPTGHRMPVLANLFGTPGRVARALGVAGLPEIRAFGEVLAQLKEPEPPRGVK
                     DLWGRRDLLKTLWSMAPSRVARPPCQQIVREGAEVDLARLPIQHCWPGDVAPLVTWGL
                     TITRGPNKPRQNLGIYRQQVIGRNQLIMRWLSHRGGALDFAEHCRQHPGQPYPVAVAI
                     GADPATILGAVTPVPDALSEYQFAGLLRGARTEVADALGVPLQVPAQAEIVLEGHIHP
                     DASHKSGWQHALEGPYGDHTGYYNECAEFPVFTVERITQREGALYHSTYTGKPP"
BASE COUNT          444 a         1198 c         1051 g          454 t
ORIGIN      
        1 accgaggtgt tcgcctatgg ccgcatgccg ctggcgtttt cggcgcgctg cttcaccgcg
       61 cgccactaca acctgcccaa ggacgactgc ggttttgcct gcatccagca ccccgacggg
      121 cagttgctga agacgcgcga gggcgaggcc ttcctggtgc tcaacggcat ccagacgcag
      181 tcggcgcgcg tctacaacct gatcggcgac ctgcccgagc tgcgcgccct gggcgtcgac
      241 gtgctgcgcc tgtcgccgca gtcgcagcac atggccgaca tcgtcgccgc cttcgacgcc
      301 gcgcgccgcg ccgacacgcc cgaccccgac gcgctggcac gcctgcgccc gatgatgccc
      361 gacgaaccct gcaacggcta ctggcacggg cgctccggca tggacctgat cgagccggcc
      421 ctggcctgaa cgccggcgca cgtgcgagga gcctgtcatg accacgcccc ccctgcccac
      481 ccccttcacc ctgccgcgtc cggtgggtgc gctgctgtcg tgcctgccgg cctaccccgg
      541 ctcggtgctg ctggtcagcg ccatcaacct ggcgctggcg cgccagctgc ccgaggacgt
      601 gacggcgctg ctgaaggaca agcgcctgtc gatccgcgtg cgcgacgccc gcctgggttt
      661 cgacttcacc tggaacggcc ggcgctttgc ccccagcgcg ccgcacgccg agcccgacct
      721 ggccatcagc gccaacgcgc aggactttct gctgctggcc cagcgccgcc aggacccgga
      781 cacgctgttc ttcaaccgcc gcctggtgat ggagggcgac accgagctcg gcctggtggt
      841 caagaacgcg ctcgacgcgc tggagctgcc ggtgctcgac ccggcggcct ggagcccgcg
      901 cgccgtgctg gcgcgctggg cgccggggct ggccgagcgg ctgccgccct tcccgccctt
      961 ttcggcgccg cgccgatgaa cccgtccggc acgcacgacc acgcgccgct ggtgtactcc
     1021 tgctcgggct gctccagcgc cgcgcaactg gccaaccacg tggcgctgca gctcgaccgc
     1081 cgcggcgtcg ccgagatgtc ctgcatcgcc ggcgtgggcg gcgacgtgcc caagctggtc
     1141 aggctggcca ccagcggccg gcccatcgtg gcgctcgacg gctgcccgct ggcgtgcgtt
     1201 cgaagctcgc tggcacgcca cggggtggcg ccggcgcgcc actatcagct gcaccagtac
     1261 ggcgtgaaga agcgcacgca ccaggacttc gacccggccc aggcggccga ggtgctggcg
     1321 cgcatcgaag ccgacctgcg gcagcatccg ctggcgcccg cccatgactg agctgcgccg
     1381 cgtcatcgtc ggcatcaccg gcgccagcgg cgccatttat ggcgtgcggc tgctgcaggt
     1441 gctgcgcgag ctggccagcg tcaccacgca cctggtggtg tcggacgccg gctggcgcag
     1501 cctgcgctac gagctggacc tggcaccggc gcaggtgcag gccctggccg acgaggcgca
     1561 cgacatccgc aacatcggcg cctgcattgc cagcggctcc ttcgtcacgc tgggcatggt
     1621 ggtggcaccc tgctccatgc gcacgctggc ggcgattgcc cacggcctgg gcgacaacct
     1681 gctgacgcgc gccgccgacg tggtgctgaa ggaacgccgc cgcctggtgc tgctggcgcg
     1741 cgagtcgccc ctgcacctgg gccacctgcg caacatggtg gcggtgaccg agctgggtgc
     1801 catcgtcagc ccgccggtac cggcgttcta caagcgcccg cgcagcctgg aagacatcgt
     1861 cgatcacagc gtggcacgcg cgctggacgc cctgggcatc gaacacaagc tcagcacccg
     1921 ctggccgggg ctgccgacgg actattgaaa tcatagctgc cggcgcacgt ccgacaagcg
     1981 ctgcagcatt gttttgcact tatgtccagc tgcctcgcag gcccctcaac ccaccctctc
     2041 tccagaggga cgggggcgat cgcgcgccgt ggttttcact gccactccct ccgggagagg
     2101 actggggtga gggccggcag gcgccatcag ggccgcacct gacaagccgc ccctgaccat
     2161 gccctaccgc gacctgcgtg acttcatggc ccagctggag gccgcaggcg agctgcgccg
     2221 cattgccgag ccggtctcgc cgcacctgga gatgacggcc cttgccgacc gcgtgctgcg
     2281 cgcgggcggc ccggccctgc tgtttgaaaa ccccacgggc caccgcatgc cggtgctggc
     2341 caacctgttc ggcacacccg gccgcgtggc gcgcgcgctg ggcgtggcgg gtttgcccga
     2401 aatccgcgcc tttggcgagg tgctggccca gctcaaggag cccgagccgc cacgcggcgt
     2461 caaggacctc tggggccggc gcgacctgct caagaccctg tggagcatgg cgccctcgcg
     2521 cgtagcccgc ccgccctgcc agcagatcgt gcgggagggc gccgaggtcg atctggcacg
     2581 cctgcccata cagcattgct ggcccggcga cgtggcgccg ctcgtcacct ggggcctgac
     2641 catcacgcgc gggccgaaca agccgcggca gaacttaggc atctaccgcc agcaggtgat
     2701 cggccgtaac cagctcatca tgcgctggct ctcgcaccgc ggcggggcgc tcgattttgc
     2761 cgagcattgc cgccagcacc ccggccagcc ctaccccgtg gccgtggcca ttggcgcgga
     2821 cccagcgacc atcctgggcg cggtcacccc cgtgcccgac gctttgtccg aataccagtt
     2881 tgctggcctc ttgcgcggcg cgcgcaccga ggtggccgac gcgctgggcg tgcctttgca
     2941 agtgccggcc caggcggaga tcgtgctcga gggccatatc caccccgacg ccagccacaa
     3001 gagcggctgg caacacgcgc tcgaagggcc gtatggcgac cacacgggct actacaacga
     3061 atgcgccgag ttcccggtgt tcaccgtcga gcgcatcacc cagcgcgagg gcgcgctcta
     3121 ccacagcacc tacaccggca agccgcc
//
LOCUS       DAOOOG010000238         3128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_37761_length_3128_cov_23.986007, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000238 DAOOOG010000000
VERSION     DAOOOG010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37761_length_3128_cov_23.986007"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PK242_12610"
     CDS             complement(<1..530)
                     /locus_tag="PK242_12610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ94904.1"
                     /translation="MIEQTSSNNHHEIRDAIRALCAQFPDEYFRKVDEQRAYPAEFVD
                     ALTRAGWMAALIPHEYGGSGLGLTEASVIMEEINRAGGNSGACHGQMYNMGTLLRHGS
                     AAQKEKYLPKIASGEWRLQSMGVTEPTTGTDTTKIKTSAVKKDGRYVVNGQKVWISRV
                     QHSDWMILLARTTPLA"
     gene            complement(605..1498)
                     /locus_tag="PK242_12615"
     CDS             complement(605..1498)
                     /locus_tag="PK242_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOZ94905.1"
                     /translation="MPGPCTAPLDATTVAHLQQWCGRGETWHDQLHAAPLRGLSATLD
                     RDDPSPAPGSALPPLGHWLYFLPTARQSELGADGHPRRGGFLPPVPLPRRMWAGGRLA
                     WQADNPLCVGQQVERRSTIRSVTHKAGRTGELVFVLVEHQIHNAHGLALTEAHDIVYR
                     SAPQPDDPVPPPQTPPLAGQQAWSRTIVPDDVLLFRYSALTFNGHRIHYDRRYATEVE
                     GYPGLIVHGPLIATLLLDLLRRERPRSQVARFEFRAVRPTFDLHPFSVHGAPSAEGHC
                     VRLWAQDHEGFLTMQASAELA"
     gene            complement(1547..3001)
                     /locus_tag="PK242_12620"
     CDS             complement(1547..3001)
                     /locus_tag="PK242_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOZ94906.1"
                     /translation="MNDSCFHSLRRLPDKRHSPVLSSVAAWVAAALLTACSLAPPERA
                     PELPLAGGWKGAAADGWVSTAAHQSWREGRWWTLFGDPDLDALMARLDIDNASLAQAV
                     AQVAQAEALLRQAEAQRAPVLGAQAGVQRSGRPARGSADLGLTASWAPDLWGRLGDAV
                     RAQGAQLQVSQADLAGARLAAQGSLAQAYFALRAADAEQALLDDIITGYERAATITGN
                     RYRAGIAAHTDLLQAQSTLDNARATRAGLQRNRASYEHAIAVLLGQPPARFTLAPAPW
                     IDQVPTVPPELPASLLLRRPDVAAAERAVAAANARIGVARAAWFPSLNLSASLGGGAS
                     GLSQLVSAPTLAWSLGAALAQTLFDAGARDAAIDQARAAHLAASAAYRQRALAAMGQV
                     EDQLSALATLATQIGHVSAAAAAAAAAEQRIMNSYQAGLSAYTNVVSAQASTLGARRA
                     LLQLQLQRQQAALALIEALGGGWQAPWAAAGGAP"
BASE COUNT          409 a         1082 c         1194 g          443 t
ORIGIN      
        1 tcggccagcg gcgtggtgcg cgccagcaga atcatccagt cgctgtgctg cacgcgcgag
       61 atccagacct tctggccgtt gaccacgtag cgcccgtcct tcttcacggc gctggtcttg
      121 atcttggtgg tgtcggtgcc ggtggtgggc tcggtcacgc ccatggactg caagcgccat
      181 tcgccgctgg ctatttttgg caggtacttt tccttctgcg ccgcgctgcc atggcggagc
      241 agcgtgccca tgttgtacat ctggccgtgg caggcgccgc tgttgccgcc cgcgcggttg
      301 atctcctcca tgatcacgct ggcctcggta agccctaagc ccgagccgcc atattcgtgc
      361 gggatcagcg cggccatcca gccggcgcgg gtcagcgcat cgacgaactc cgccgggtag
      421 gcgcgttgct cgtccacctt gcggaagtat tcgtccggaa actgggcgca cagcgcgcgg
      481 atcgcgtcgc ggatctcgtg gtggttgttg gagctggtct gttcgatcat gccggtggtg
      541 gtcggtgttt cagccctctg gcgcgctgtc agcaagcgcc agcagctatc aaatatatag
      601 tgattcaggc cagttcggcg ctggcctgca tggtgagaaa gccctcgtgg tcctgcgccc
      661 acagccgcac gcagtggcct tccgccgacg gcgcgccatg cacgctgaaa ggatgcagat
      721 cgaaggtcgg acgcacggcg cgaaactcga agcgcgccac ctgggaccgg ggccgttcgc
      781 ggcgcagcag atcgagcagc agcgtggcga tcagcgggcc gtgcacgatg aggccagggt
      841 agccctcgac ctcggtggcg tagcggcggt cgtaatggat gcggtggccg ttgaaggtca
      901 gggccgagta gcgaaacagc agcacgtcgt ccggcacgat ggtgcgcgac caggcctgct
      961 ggccggccag cggcggggtc tgcggcggcg gcaccgggtc gtcgggctgg ggcgccgaac
     1021 ggtagacgat gtcgtgcgcc tccgtcagcg ccaggccgtg ggcgttgtgg atctggtgct
     1081 cgaccagcac gaacaccagc tcacccgtgc gccccgcctt gtgcgtgacc gagcgaatgg
     1141 tggagcgccg ctcgacctgc tggcccacgc acagcgggtt gtccgcctgc caggccaggc
     1201 ggccaccggc ccacatgcgg cgcggcagcg gcaccggcgg cagaaagccg ccgcgcctgg
     1261 gatggccgtc ggcgcccagc tcgctctggc gggcggtggg cagaaagtac agccagtgcc
     1321 ccagcggcgg cagcgcactg cccggtgcgg gcgacgggtc gtcacggtcc agcgtggccg
     1381 acaggccgcg cagcggcgcc gcgtgcagct gatcgtgcca ggtctcgccg cggccgcacc
     1441 actgctgcag gtgggcgacg gtggtggcgt caagaggggc ggtgcaaggg ccgggcatgc
     1501 gtccatcatg gcgtggcgac gcgcccgcgg caagcgccgg cgggggtcat ggcgcgccac
     1561 cggccgctgc ccagggcgcc tgccagccgc cacccagggc ctcgatcagc gccagcgccg
     1621 cctgctggcg ctgcagttgc agctgcagca gcgcacggcg cgcacccagg gtgctggcct
     1681 gggcgctgac gacgttggtg taggccgaca agccggcctg gtagctgttc atgatgcgct
     1741 gctcggcggc ggccgccgcg gcggcggcgg cgctcacgtg gccgatctgc gtcgccagcg
     1801 tggccagtgc gctgagctgg tcttccacct ggcccatggc ggccagggcg cgctgacggt
     1861 aggcggcgct ggccgccagg tgcgcggccc gcgcctggtc gatggcggcg tcgcgcgcgc
     1921 cggcgtcgaa cagggtctgc gccagcgccg cgccgagcga ccaggccagg gtgggcgccg
     1981 acaccagttg cgacagcccg ctggcgccgc caccgagcga ggcgctgagg ttgaggctgg
     2041 ggaaccaggc cgcccgcgcc acgccgatgc gcgcgttggc cgcggcaacc gcgcgctcgg
     2101 ccgccgccac gtcggggcgg cgcagcagca ggctggccgg cagctccggc ggcaccgtgg
     2161 gcacctggtc gatccagggc gcgggcgcga gcgtgaagcg ggccggcggc tggcccagca
     2221 gcacggcaat ggcgtgctcg tagctggcgc ggttgcgctg caggccggcg cgcgtggcac
     2281 gggcgttgtc cagcgtgctc tgggcctgca gcaggtcggt gtgggcggca atgccggcgc
     2341 gatagcggtt gccggtgatg gtggcggcgc gttcgtagcc ggtgatgatg tcgtccagca
     2401 gcgcctgttc ggcgtcggcc gcgcgcaggg cgaagtaggc ctgcgccagg ctgccctggg
     2461 ccgccagccg cgcgccggcc agatcggcct ggctgacctg cagctgggcg ccctgggcgc
     2521 gcacggcgtc gcccagccgg ccccacaggt cgggcgccca gctggcggtg aggcccaggt
     2581 cggccgaacc gcgcgccggc cggccgctgc gctgcacccc ggcctgcgcg cccagcaccg
     2641 gggcgcgctg cgcctcggcc tggcgcagca gcgcctcggc ctgcgccacc tgggccacgg
     2701 cctgcgccag gctggcgttg tcgatgtcca ggcgcgccat cagtgcgtcg agatcggggt
     2761 cgccgaacag cgtccaccag cgcccttcgc gccaggactg gtgcgcggcg gtgctgaccc
     2821 atccgtcggc ggcggcgccc ttccagccgc cggccagcgg cagctcgggc gcgcgctcgg
     2881 gcggcgccag gctgcaagcg gtcaacagcg cggccgccac ccatgcggca accgaagaca
     2941 aaacagggct atggcgcttg tctggcaagc gccgcaagct atgaaaacag gagtcgttca
     3001 tgtcgtgtcc gcccctggcg cggcgaccac gccgcgcgcc agatgggcct gatcggggcg
     3061 ccggcggcgc agtcggtcga gcagcacata gaccaccggc gtggtcagca gggtcagcaa
     3121 ctggctgg
//
LOCUS       DAOOOG010000239         3104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_38150_length_3104_cov_29.627419, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000239 DAOOOG010000000
VERSION     DAOOOG010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38150_length_3104_cov_29.627419"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..694)
                     /locus_tag="PK242_12625"
     CDS             complement(32..694)
                     /locus_tag="PK242_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ94907.1"
                     /translation="MTRKAPRRTAERILVHSLALFNRFGEPNVSTTMISAELRISPGN
                     LYYHYPSKDELINTLFDQYQHALLELLAAAEDVHEAEDAWFFLHSLFELIWQYRFFYR
                     DLNHLLSRNRHLETRFPDLLARKKAALRALLDALADAPAAAHDERARDAVAASMVVLL
                     TYWLSYEYVLQPRSAMEPENAQDALLRGAWHVLGLLMPHLGEAARLQMLTLIDAYRPD
                     AG"
     gene            complement(708..1868)
                     /locus_tag="PK242_12630"
     CDS             complement(708..1868)
                     /locus_tag="PK242_12630"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOZ94908.1"
                     /translation="MIATLVVVALHAALLLGLPHLGQVTRAGQDAGSFVTRLIAPPAA
                     VAVPAPVAAAPPPRPTEPTRPRPPPTRAVDHAPKVAPTAAPPTAPPAQVSNRSDAPGH
                     TAPAVSQLGPKPAGASFGGQSAPVPIEPAATATEAATALAFAPQAGDAPTRVPRAAEL
                     SYRTRGHIGGQPFEVPTTLHWRQDGQWYEIRWGLYSPRVGEQTRYAVGLLSPQGLLPL
                     RAELRTPELRDMRLDYARQRIHLSASDTEVPLAAGTQDRLGVLIQLGARIAGDPERYP
                     PGQTIELPAVRPTGIGRWRFVVEADETLEALKGQNLPTVRLVHAAQDDQDVRIELWLG
                     RALDYLPVRLRITEAGGDTVEHDLVTAWARPTPVAPPAPQAPPAGPPAAGTN"
     gene            complement(1963..2436)
                     /locus_tag="PK242_12635"
     CDS             complement(1963..2436)
                     /locus_tag="PK242_12635"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOZ94909.1"
                     /translation="MATRPRTSTRTTPPPDSPAPATATPLGMPAHNIWLAGLGALSGA
                     QANAQAEGSKAFEALVKQGLEWQARTQALAKEKWAEAAERMGAMTAQATGGVGSWDRL
                     GGIFEERVARALASMGMPSASEVAQLQARVEALETALNALQSGAPQPRPARARKR"
     gene            2597..>3104
                     /locus_tag="PK242_12640"
     CDS             2597..>3104
                     /locus_tag="PK242_12640"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOZ94910.1"
                     /translation="MILVTGGAGFIGSNFVLDWLAASDEPVLNLDKLTYAGNLANLAA
                     LQGDARHRFVQGDIGDRALLERLLAEHRPRAIVNFAAESHVDRSIHGPEDFIQTNVLG
                     TFRLLEAARHYWSALPATEKEAFRFLHVSTDEVYGTLAPQDPAFAEDHKVEPNSPYSA
                     SKAASDHLV"
BASE COUNT          417 a         1061 c         1173 g          453 t
ORIGIN      
        1 cacggccagc ggtccacggc cgctttcagg ctcaacccgc gtcgggccga taggcgtcga
       61 tcagcgtcag catctgcagg cgcgcggctt cgcccaggtg cggcatcagc agccccagca
      121 catgccaggc gccacgcaac agggcgtcct gggcgttctc gggctccatg gcgctgcgcg
      181 gttgcagcac gtattcgtag ctcagccagt aggtcagcag caccaccatg ctggcggcca
      241 cggcatcgcg cgcgcgttcg tcgtgcgccg ccgcgggcgc gtcggcgagc gcatcgagga
      301 gggcgcgcag ggccgccttc ttgcgcgcca gcagatcggg gaatcgggtc tccaggtgcc
      361 ggttgcgcga cagcaggtgg ttcaggtcgc ggtagaagaa gcggtactgc cagatcagct
      421 cgaacagcga atgcaggaag aaccacgcgt cctcggcctc gtgcacgtcc tcggcggcgg
      481 ccagcagttc gagcagggcg tgttggtact ggtcgaacag ggtgttgatc agctcgtcct
      541 tggacgggta gtggtagtac aggttgcccg ggctgatgcg cagctcggcc gaaatcatgg
      601 tggtcgagac gttgggctcg ccaaagcggt tgaacagcgc cagcgaatgc accaggatgc
      661 gttcggcggt gcggcggggc gctttgcggg tcatggcgcg cgcgctttca gttggtgccg
      721 gccgccggcg ggccggcagg cggggcctgc ggggcgggcg gtgccaccgg ggtcggcctg
      781 gcccaggccg tgaccaggtc gtgttcgacc gtgtcgccac cggcttcggt gatgcgcagg
      841 cgcaccggca gatagtccag cgcgcggccg agccacagct cgatgcgcac gtcctggtcg
      901 tcctgcgccg cgtgcaccag gcgcaccgtg ggcaggttct ggcccttgag ggcctccagc
      961 gtctcgtcgg cctcgaccac gaagcgccag cgcccgatgc cggtgggccg cacggcgggc
     1021 agctcgatgg tttggccggg cgggtagcgt tcgggatcgc cggcgatccg tgcacccagc
     1081 tggatcagca cgcccaggcg gtcctgggtg ccggcggcca gcggcacctc ggtatcgctg
     1141 gcgctcaggt ggatgcgctg gcgcgcgtag tccaggcgca tgtcgcgcag ttcgggcgtg
     1201 cgcagctcgg cccgcagcgg cagcagaccc tggggcgaca gcagaccgac ggcgtagcgc
     1261 gtctgctcgc cgacgcgtgg gctgtacagc ccccagcgga tctcgtacca ctggccgtcc
     1321 tggcgccagt gcagcgtggt cggcacctcg aacggctggc cgccgatgtg gccgcgcgtg
     1381 cggtagctca gttcggcggc acgcggcacg cgggtcggcg catcgcccgc ctgtggcgcg
     1441 aacgccagcg ccgtcgccgc ttcggtcgcc gtggccgccg gctcgatggg gacgggggcc
     1501 gactggccgc cgaaggaggc gcccgccggt ttgggcccca gctggctgac cgcgggcgcc
     1561 gtgtggccgg gcgcatcgga gcggttgctg acctgcgccg gcggcgctgt gggcggggcg
     1621 gcggtggggg ccaccttggg ggcatggtcc accgcccggg tgggtggcgg ccgcggacgc
     1681 gtaggctcgg tcgggcgcgg cggtggggcg gctgcaaccg gcgccggcac ggcaacggcg
     1741 gctggcgggg cgatcaggcg cgtcacgaag ctgcccgcat cctgcccggc gcgtgtgacc
     1801 tggcccaggt ggggcaagcc cagcagcagc gccgcatgca gcgccaccac gaccagggtc
     1861 gcaatcacca gttgccgcgt gggagtggca ggcaggggag cgggtgacag ggcagggttc
     1921 aacggcaggc agcgtacggg ggcggtcgga gcacggcggc gttcagcgct ttctggcgcg
     1981 cgccgggcgc ggctggggcg caccgctctg cagcgcgttc aaggcggttt ccagcgcctc
     2041 cacgcgcgcc tgcagttgcg ccacctcgct ggccgagggc atgcccatgc tggccagcgc
     2101 gcgcgccaca cgctcctcga agatgccgcc cagccggtcc cacgacccca cgccgcccgt
     2161 ggcctgggcg gtcatggcgc ccatgcgctc ggcggcttcg gcccattttt ccttggccag
     2221 cgcctgcgtg cgcgcctgcc attccaggcc ctgcttgacc agcgcctcga aggccttgct
     2281 gccttcggct tgggcattgg cctgggcacc cgacagcgcg cccagcccgg ccagccagat
     2341 gttgtgggcc ggcatgccca ggggtgtggc tgtggctggc gccgggctgt ccggcggcgg
     2401 ggtggtgcgg gtgctcgtgc ggggtcgggt ggccatgggc gctcctgggt ggctgggcgg
     2461 tgcgggtcgc agccgatact gtagcccttc cgtgggctgg cggggcgcat gaaataatcg
     2521 ccgctttccc cgggcagcgt cgcccctgca ggccagcggc acccggacag acggcagatt
     2581 tcagagacgc aagcacatga ttctggtcac cggcggcgcc ggcttcatcg gcagcaactt
     2641 cgtgctggac tggctggcgg ccagcgacga gccggtgctc aacctcgaca agctcaccta
     2701 cgccggcaac ctggccaacc tggcggcgct gcagggcgat gcgcgccacc gcttcgtgca
     2761 gggtgacatc ggcgaccgcg cgctgctgga gcggctgctt gccgagcacc ggccgcgcgc
     2821 catcgtcaac ttcgccgccg aaagccatgt ggatcgctcc atccacggcc cggaggactt
     2881 catccagacc aacgtgctcg gcaccttcag gctgctggag gccgcaaggc actactggag
     2941 cgcgctgcca gctacagaaa aagaagcgtt ccggttcctg cacgtgagca ccgacgaggt
     3001 ctacggcacg ctggcgccgc aggatccggc ctttgccgaa gaccacaagg tcgagcccaa
     3061 cagcccctac agcgccagca aggccgccag cgaccacctg gtgc
//
LOCUS       DAOOOG010000240         3088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_38415_length_3088_cov_28.424332, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000240 DAOOOG010000000
VERSION     DAOOOG010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38415_length_3088_cov_28.424332"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /gene="sucC"
                     /locus_tag="PK242_12645"
     CDS             complement(<1..782)
                     /gene="sucC"
                     /locus_tag="PK242_12645"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOZ94911.1"
                     /translation="MKIHEYQGKEILRQFGVPVPRGIPAFTVQEAVEAAQKLGGPVWV
                     VKAQIHAGGRGKGGGVKVAKSLEQVKELAGQILGMQLKTHQTGPEGQKVRRLYIEDGA
                     DIQKEYYLSVVTDRASQKVAFIASSEGGMDIEEVAHSTPEKIVKVFVDPLVGFTDAQG
                     QELARGVGMPADSAAQFVDVCRKLYQCYMDTDASLVEINPLNRDSKGNIIALDAKFNF
                     DSNALFRHPEIVALRDLDEEDPAEIEASKFDLAYISLDGNIG"
     gene            980..1894
                     /gene="argC"
                     /locus_tag="PK242_12650"
     CDS             980..1894
                     /gene="argC"
                     /locus_tag="PK242_12650"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOZ94912.1"
                     /translation="MAKVFIDGEAGTTGLQIRERLQGMAGVELLSIDPARRKDPQAKR
                     DLMAAVDLVVLCLHDDAARESVAMIDALGERRPRIIDASTAHRVAPGWVYGFPELSAQ
                     QRAAVQAAERVANPGCYATGAIALLRPLVDAGLLPPEHPVALPSVSGYTGGGRQMIEA
                     YEAGNAPPYELYALGLKHKHIPEIMRYGRVTRRPIFVPAVGNFDQGMLVELPLHLDLL
                     PGQPSAEQLLRTYRAHYAGAEAVSVEAAPESGKLDAVALKGTNQLEIRVFANPEAAQA
                     VCVARLDNLGKGASGAAVQNLKLMLGLD"
     gene            complement(2158..2637)
                     /gene="recX"
                     /locus_tag="PK242_12655"
     CDS             complement(2158..2637)
                     /gene="recX"
                     /locus_tag="PK242_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HOZ94913.1"
                     /translation="MARAPLSLKARALRYLAAREHSRAELATKLARHATEHESPAAIE
                     RVLDELAAKGFIDEARVAESVLHRRAPRLGSARVLHELRTKGLPEAVVADAARQLQGS
                     EFDRALAVWRRRFAAPPGDAGERARQLRFLAGRGFSAEVAWRVLSAAAQATGPSNGG"
     gene            complement(2709..>3088)
                     /gene="recA"
                     /locus_tag="PK242_12660"
     CDS             complement(2709..>3088)
                     /gene="recA"
                     /locus_tag="PK242_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653815.1"
                     /note="catalyzes the hydrolysis of ATP in the presence of
                     single-stranded DNA, the ATP-dependent uptake of
                     single-stranded DNA by duplex DNA, and the ATP-dependent
                     hybridization of homologous single-stranded DNAs; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOZ94914.1"
                     /translation="YASVRLDIRRIGTIKKGDEAIGNETRVKVVKNKVSPPFKQAEFD
                     ILFGEGISREGEIIDMGVTAKVLDKSGAWYAYNGEKIGQGRDNAREFLRENPELAREI
                     ENKVRESLGVAQRPAALADADEV"
BASE COUNT          463 a         1064 c         1043 g          518 t
ORIGIN      
        1 cagccgatgt tgccgtccag gctgatgtag gccaggtcga acttggaggc ttcgatctcg
       61 gccgggtctt cctcatcgag gtcgcgcagg gcgacgatct cggggtggcg gaacagcgcg
      121 ttgctgtcga agttgaactt ggcgtccagg gcgatgatgt tgcccttgct gtcgcggttc
      181 agcgggttga tctcgaccag cgaggcgtcg gtgtccatgt agcactggta gagcttgcgg
      241 cacacgtcca cgaactgcgc cgccgagtcg gccggcatgc ccacgccgcg cgccagttcc
      301 tggccctgcg cgtcggtgaa gcccacgagc ggatcgacga acaccttgac gatcttctcg
      361 ggcgtgctgt gcgccacttc ctcgatgtcc atgccgccct cgctggaggc gatgaaggcc
      421 accttctggc tggcgcggtc ggtcacgacc gacaggtagt attccttctg gatgtcggca
      481 ccgtcctcga tgtacaggcg gcgcaccttc tggccctcgg ggccggtctg gtgggtcttg
      541 agctgcatgc ccaggatctg gccagccagt tccttgacct gttcgagcga cttggccacc
      601 ttgacgccgc cgcccttgcc gcgaccgccg gcgtggatct gggccttgac gacccacacg
      661 gggccgccca gcttctgcgc cgcctcgacg gcttcctgaa cagtgaacgc gggaatgccg
      721 cgcggcacgg gcacgccgaa ttgacgcagg atctccttgc cctggtactc gtggatcttc
      781 atgaggcccc tttgaggatg ggtgagtgag tggaaagccc gtccgccatg ccggacggtg
      841 cgcaaataac acggccgcac ccacgctacg actcgaagca ggcgtcagtc gaggcctgcg
      901 aatgtatcat gttgcggcgc accaattctc ccactggggc ttacgtcgcc ttgacgtcgg
      961 ccacttgaaa ggaccgccca tggccaaggt tttcattgac ggcgaagccg gcaccaccgg
     1021 tctgcagatt cgcgagcgcc tgcagggcat ggccggcgtg gaactgctca gcatcgatcc
     1081 ggcgcggcgc aaggacccgc aggccaagcg cgacctcatg gcggcggtcg atctggtcgt
     1141 cctgtgcctg cacgatgacg cggcgcgcga gtcggtggcg atgatcgacg cgctgggcga
     1201 gcgccggccg cgcatcatcg acgccagcac ggcgcaccgg gtggcgcccg gctgggtgta
     1261 tgggtttccc gagctgtcgg cgcagcaacg cgccgccgtg caggccgccg agcgggtggc
     1321 caacccgggc tgctacgcca ccggcgccat cgccctgctg cgcccgctgg tggacgccgg
     1381 cctgctgccg ccggagcatc cggtggcgct gccctccgtg agcggctaca ccggcggcgg
     1441 gcgccagatg atcgaagcct acgaggccgg caacgccccg ccctacgagc tgtacgcgct
     1501 cggcctgaag cacaagcaca tcccggagat catgcgctac ggccgcgtca cgcgccgccc
     1561 gatcttcgtg ccggcggtgg gcaatttcga tcagggcatg ctggtggagt tgccgctgca
     1621 cctggacctg ctgcccggcc agcccagcgc cgagcagctg ctgcgcacct accgcgccca
     1681 ctacgccggc gccgaggcgg tcagcgtgga ggcggcgccc gaatccggca agctggatgc
     1741 ggtggccctc aagggcacca accagctgga aatccgcgtg ttcgccaacc ccgaggccgc
     1801 gcaggcggtg tgcgtggcgc gactggacaa cctgggcaag ggtgccagcg gagcggcggt
     1861 gcagaacctg aagctgatgc tcggattgga ctgagcctga aaacagccgc cgcaccgccc
     1921 ggcgatgccg gcgagcgtgc gcgccagcaa atgaccctag gaggctgtgg gacttggaaa
     1981 tcgccggctg caaatcgacc gcagcggccc atttttttcg tctttttgcc caatagctcg
     2041 actcttgggc tgcaaatccg gcaaaaactg ggctcgccgg ggccgatttt ggctacgccg
     2101 cgcttcgaga acgctttcgg cgctccaagc ccgacagccg cctaggatga cagacggtca
     2161 accgccgttg ctggggcctg tcgcctgcgc cgccgcactc aacacgcgcc aggcgacttc
     2221 tgcgctgaag ccgcgccccg ccaggaagcg cagctggcgc gcacgctcgc cggcatcgcc
     2281 gggtggtgcg gcgaagcggc ggcgccagac cgccagcgca cggtcgaact cgctgccctg
     2341 caactggcgc gccgcgtcgg cgaccaccgc ctcgggcaag cccttggtgc gcagctcgtg
     2401 cagcacgcgc gcgctgccca gacggggcgc acgacggtgc agcaccgatt cggccacccg
     2461 cgcctcgtcg atgaagccct tggccgccag ctcgtccagc acacgctcga tcgccgcggg
     2521 cgattcatgc tcggtcgcat ggcgcgccag cttggtcgcc agttcggcgc gtgaatgttc
     2581 gcgcgccgcc aggtagcgca gcgcgcgcgc cttcagcgaa aggggcgccc tggccatggc
     2641 gtcagttggc gatcacccgc gcggccggcc gtgccgggca acgctgacgg gaccggctca
     2701 ggatggcctc aaacctcatc ggcgtcggcc agcgccgccg gtcgctgggc cacgcccagc
     2761 gactcgcgga ccttgttttc gatctcgcgc gccagttcgg ggttttcgcg caggaactcg
     2821 cgcgcgttgt cgcggccctg gccgatcttt tcgccgttgt aggcgtacca ggcgccgctc
     2881 ttgtcgagca ccttggcggt cacgcccatg tcgatgatct cgccctcgcg cgaaatgccc
     2941 tcgccgaaca ggatgtcgaa ttcggcctgc ttgaagggcg ggctgacctt gttcttgacc
     3001 accttgacgc gggtttcgtt gccgatcgct tcatcgccct tcttgatggt gccgatgcgg
     3061 cggatgtcca ggcgcaccga ggcgtaga
//
LOCUS       DAOOOG010000241         3087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_38431_length_3087_cov_33.471966, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000241 DAOOOG010000000
VERSION     DAOOOG010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38431_length_3087_cov_33.471966"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PK242_12665"
     CDS             complement(<1..257)
                     /locus_tag="PK242_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HOZ94915.1"
                     /translation="MTAPSTESIVQQEHAQLDYSSSMSYGDYLHIDQILSAQHPLSPA
                     HDEMLFIIQHQTSELWMKLMLTEIGAAMADIRADRLPPAFK"
     gene            complement(421..1050)
                     /gene="kynB"
                     /locus_tag="PK242_12670"
     CDS             complement(421..1050)
                     /gene="kynB"
                     /locus_tag="PK242_12670"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HOZ94916.1"
                     /translation="MSRLWDISPPVHAGTPVFPGDTAYAQRWSAQIGPGCPVNVGALT
                     LSPHVGAHADAPLHYDPQGTAVGALDLEPYLGPCRVIHAIGVRPLILWEHLAHATQAL
                     PPRVLVRTYARMPADRWDPRLAAFAPDTVEHLADAGVTLIGIDTASIDPADSKSLPSH
                     QVIRRRGLRVLENLLLDEVPEGDYELIALPLKLTTADASPVRAVLRGLT"
     gene            1194..1499
                     /locus_tag="PK242_12675"
     CDS             1194..1499
                     /locus_tag="PK242_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOZ94917.1"
                     /translation="MQTELPAQPVTAAGLLLNQLPRQARAVIEHLHMPADVADCGLLL
                     RLIELGFLPGETVRIVARAGLGGEPIAVRLGGQSTFALRAREAALIGVRPLSGAQAS"
     gene            1496..>3087
                     /locus_tag="PK242_12680"
     CDS             1496..>3087
                     /locus_tag="PK242_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOZ94918.1"
                     /translation="MNPSPDAAVAPTEAGPRAVASSARPLRVALLGNPNCGKTALFNL
                     LTGSRQKVANYAGVTVERKEGRLVSPGGRELRLLDLPGAYSLSAVSLDEEITRDIVTG
                     RRAGESLPDLLVCVVDATRLRLNLRLVLEARQLGLPVLVALNMSDVAERQGVRVDRER
                     LSAELGLPVVPTVGVRPGGAQALIAALDAFRPPLIGRRPRVDDTPPGVEAVMRTQREV
                     QRLVDACVQAPEQALARDDAIDRVLLHPLWGGLFLAALLFLMFQAVFSWAEAPMGWIE
                     SGVAALGELVRAQLADGPLRSLLVDGIIAGVGSVLVFLPQILILFAFILALEDSGYLP
                     RAAFLLDRVMGRVGLSGRSFIPLLSSFACAVPGIMATRTISDWRDRLVTILIAPLMTC
                     SARLPVYALLIAAFVPARPVAGWFNLQGLVLFALYAAGILSALAMAWVYRLAARGEGG
                     RPTALLMELPAYRWPHPRQLAFGLWERASIFLRRVGGIILAVMVLLWFLSSYPAAPAG
                     AEGPAILHSFAGRIGLLLEKLLA"
BASE COUNT          384 a          995 c         1181 g          527 t
ORIGIN      
        1 ttcttgaagg ccggcggcag gcggtcggcg cggatgtcgg ccatggcggc gccgatctcg
       61 gtcagcatca gcttcatcca cagctcgctg gtctggtgct ggatgatgaa cagcatctcg
      121 tcgtgcgcgg gcgacagcgg gtgctgcgcg ctcaggatct ggtcgatgtg caggtagtcg
      181 ccatagctca tgctgctgga atagtcgagc tgggcgtgct cctgctggac gatggattcg
      241 gtgctggggg cggtcatggg ggctccttgg ggcgtggatt gcaagtcggg aacagccgaa
      301 cgcagagggc gcaaagggtt cgcagaggac gcagaagaat tcaggaaaaa gggatcggaa
      361 ttgttttgcg tctttcgcgt ttttttcgcg tccttcgcgt ccggtattca gaagccgtgt
      421 tcacgtcagg ccgcgcagca ccgcgcgcac cggcgaggcg tcggcggtgg tgagcttgag
      481 gggcagggcg atcagctcgt agtcgccttc gggcacctcg tcgagcagca ggttttccag
      541 cacgcgcagg ccgcggcggc ggatcacctg gtggctgggc agcgacttgc tgtcggccgg
      601 gtcgatgctg gcggtgtcga tgccgatcaa ggtgacgccg gcatcggcca ggtgctcgac
      661 ggtgtcgggg gcgaaggcgg ccaaccgcgg gtcccagcgg tcggccggca tgcgggcata
      721 ggtcctgacc agcacgcgcg gcggcagcgc ttgtgtggcg tgcgccagat gctcccaaag
      781 aatgagcggt cgcacgccga tggcatggat cacgcggcac gggccgaggt acggctccaa
      841 gtccagtgcg ccgacggcgg tgccctgcgg gtcgtagtgc agcggcgcgt cggcgtgcgc
      901 gccgacatgg ggcgacaggg tgagggcgcc gacgttgacc gggcagccgg ggccgatctg
      961 cgcgctccag cgctgcgcat aggcggtgtc gcccggaaag accggcgtgc cggcatgcac
     1021 cggtggcgag atgtcccaca gcctgctcat ggcgtgcacg ttatcgggtt tgaggcggtc
     1081 gtggtgtcgg ttatccgcaa cgtcatcgcc gtggcgaggg ttgatgcaga tcagggcggc
     1141 cgcagctcgg cagctcatac aatgagaatc aatctcattt gtagggatct gccatgcaga
     1201 ccgaattgcc tgcccagccg gtcaccgccg ccggcctgct gctgaaccag ttgccgcgcc
     1261 aggcgcgcgc cgtcatcgaa cacctgcaca tgccggccga cgtggccgac tgcggcctgc
     1321 tgctgcggct gatcgagctg ggcttcctgc cgggcgagac ggtgcgcatc gtggcgcggg
     1381 ccgggttggg cggcgagccg atcgccgtgc gcctgggcgg gcagtccacc tttgccctgc
     1441 gcgctcgcga ggcggcgctg atcggcgtgc gcccgctgag tggcgcgcag gcctcatgaa
     1501 cccgagtccc gacgccgctg tcgcccccac cgaggccggg ccgcgcgctg tcgccagcag
     1561 cgcccgcccg ttgcgcgtgg cgctgctggg caaccccaac tgcggcaaga cggcgctgtt
     1621 caacctgctg accggcagcc gccagaaggt ggccaactac gccggcgtga cggtggagcg
     1681 caaggaaggg cgcctggtct cgcccggcgg acgcgagctg cgcctgctgg acctgcctgg
     1741 cgcctacagc ctgagcgccg tcagtctgga cgaggagatc acgcgcgaca tcgtcaccgg
     1801 ccgccgcgcc ggcgagtcgc tgcccgacct gctggtgtgc gtggtcgacg ccacccggct
     1861 gcgcctgaac ctgcgcctgg tgctggaggc gcgccagctg ggcctgccgg tgctggtggc
     1921 gctgaacatg agcgacgtgg ccgagcgcca gggcgtgcgc gtggaccgcg agcgcctgtc
     1981 ggccgagctg ggcctgcccg tggtgcccac ggtgggggtg cgcccgggcg gtgcccaggc
     2041 gctgatcgcg gcgctggatg cctttcgccc gccgctgatc ggccgccgtc cgcgcgtgga
     2101 cgacacgccg cccggcgtcg aggcggtgat gcgcacccag cgcgaggtgc aacgtctggt
     2161 ggacgcctgc gtgcaggccc cggagcaggc gctggcgcgc gacgacgcca tcgaccgcgt
     2221 gctgctgcac ccgctgtggg gcgggctgtt cctggcggcg ctgctgttcc tgatgttcca
     2281 ggccgtgttc agctgggccg aagcacccat gggctggatc gagtcgggcg tggccgcgct
     2341 gggcgagctg gtgcgcgcgc agctggccga cggcccgctg cgcagcctgc tggtggacgg
     2401 catcatcgcc ggcgtgggca gtgtgctggt gttcctgcca cagatcctga tcctgtttgc
     2461 cttcatcctg gcgctggagg actcgggcta cctgccgcgc gcggcctttc tgctggaccg
     2521 ggtgatgggg cgggtggggc tgtcggggcg ttcgttcatt ccgctgctgt ccagctttgc
     2581 ctgcgccgtg cccggcatca tggccacgcg caccatcagc gactggcgcg accggctggt
     2641 gaccatcctg atcgcgccgc tgatgacctg ctcggcccgg ctgccggtgt atgcgctgct
     2701 gatcgccgcc ttcgtgccgg cgcgaccggt ggcggggtgg ttcaacctgc aggggttggt
     2761 gttgttcgcg ctgtatgcgg ccggcatcct gagcgcgctg gccatggcct gggtgtatcg
     2821 gctggcggcg cgcggcgaag gcgggcggcc gacggcgctg ctgatggagc tgccggccta
     2881 ccgctggccg cacccgcgcc agctggcgtt cggcctgtgg gagcgcgcca gcatcttcct
     2941 gcggcgcgtg ggcggcatca tcctggcggt gatggtgctg ctgtggttcc tgtccagcta
     3001 tccggcggcg cccgccgggg ccgaggggcc ggccattctg cacagctttg ccggccgcat
     3061 tggcctgctg ctggaaaagc tgctggc
//
LOCUS       DAOOOG010000242         3071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_38693_length_3071_cov_23.797414, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000242 DAOOOG010000000
VERSION     DAOOOG010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38693_length_3071_cov_23.797414"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /gene="rimO"
                     /locus_tag="PK242_12685"
     CDS             complement(<1..1322)
                     /gene="rimO"
                     /locus_tag="PK242_12685"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOZ94919.1"
                     /translation="MSETTTPPRIGFVSLGCPKALTDSELILTQLSAEGYQTSKTFEG
                     ADLVIVNTCGFIDEAVKESLDTIGEALTVNGKVIVTGCLGAKTIAAGADRADAGARNE
                     PENLIKHLHPSVLAVTGPHATQEVMDAVHRHLPKPHDPFLDLVPGAFGEAGLKLTPRH
                     YAYLKISEGCNHRCTFCIIPSMRGDLVSRPIGEVLKEARALFEGGVKELLIISQDTSA
                     YGVDVKYRTGFWDGRPLRTRLLELVQALGELAAPHGAWVRLHYVYPYPHVDDILPLMA
                     EGKVLPYLDVPFQHSHPEVLRRMKRPASGEKNLERIARWRQICPELVIRSTFIAGFPG
                     ETEAEFQHLLDFMREARIDRAGCFAYSPVEGAAANQLPGMLAPELREERRARFMAVAE
                     AVSTAKLRERVGDTLQVLIDAAPALGRRGGLGRSYADAPEIDGVVHLLP"
     gene            complement(1361..1927)
                     /gene="phaR"
                     /locus_tag="PK242_12690"
     CDS             complement(1361..1927)
                     /gene="phaR"
                     /locus_tag="PK242_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HOZ94920.1"
                     /translation="MSPSKARTPAQPARDASAGAPQRVIKKYPNRRLYDTTTSSYITL
                     AQVRELVMAHQPFVVRDAKTGDDLTRSILLQIILEEEAGGAPMFTENMLANIIRFYGH
                     AMQGFMGSYLEKNIQAFMEIQSRLAEQSAGLSPELWAQFMRMQSPMMQGMMGSYLEQS
                     KNLFLQAQEQMSRQTEHMLGAFGIKRSG"
     gene            2358..2651
                     /locus_tag="PK242_12695"
     CDS             2358..2651
                     /locus_tag="PK242_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOZ94921.1"
                     /translation="MWKLGRLLTLFRKELLLAWGLLRNPGAPLSSKLVTLLALLYVIS
                     PVDLVSDLIPVLGWLDDGLVAWLLWRLALRFAPAELVATLRGKLDARLRPAPR"
     gene            complement(2655..>3071)
                     /locus_tag="PK242_12700"
     CDS             complement(2655..>3071)
                     /locus_tag="PK242_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HOZ94922.1"
                     /translation="MAQLEVPIITTIIGEGGSGGALAIAVADQVLMLQYAMYSVISPE
                     GCASILWKTSDKAQDAAEALGITAHRLKALGMVDKIVSEPVGGAHRDPRHMGTLLKRA
                     LGDAWRQVSDLKVKELLERRYERLMSYGRFNDTKER"
BASE COUNT          445 a         1018 c         1060 g          548 t
ORIGIN      
        1 ggcagcaaat gcaccacgcc gtcgatctcg ggcgcgtcgg catagctgcg ccccagcccg
       61 ccgcgccgcc ccagggccgg cgcggcatcg atcagcacct gcagggtgtc gcccacgcgt
      121 tcgcgcagct tggcggtgga cacggcttcg gccaccgcca tgaagcgcgc gcggcgctct
      181 tcgcgcagct cgggcgccag catgccgggc agctggttgg cggcggcgcc ttcgaccggt
      241 gagtaggcaa agcagccggc gcggtcgatg cgcgcctcgc gcatgaagtc cagcaggtgc
      301 tggaactcgg cttcggtctc gcccggaaag ccggcgatga aggtggagcg gatcaccagt
      361 tcgggacaga tctgacgcca gcgcgcgatg cgctccaggt tcttctcgcc gctggccggg
      421 cgcttcatgc ggcgcagcac ttcggggtgg ctgtgctgga acggcacgtc caggtacggc
      481 agcaccttgc cttcggccat cagcggcagg atatcgtcca catgggggta cgggtacacg
      541 taatgcaagc gcacccaggc gccatgcggc gcggccagct cgcccagcgc ctgcaccagc
      601 tccagcaggc gcgtgcgcag cgggcggccg tcccagaagc cggtgcggta cttcacatcc
      661 acgccgtagg ccgaggtgtc ctggctgatg atcagcagct ccttgacgcc accttcgaac
      721 agcgcgcgcg cttccttcag cacctcgccg atggggcggc tcaccaggtc gccgcgcatc
      781 gaggggatga tgcagaaggt gcagcggtgg ttgcagccct cgctgatctt caggtaggcg
      841 tagtggcgtg gcgtgagctt caggccggct tcgccgaagg cgccgggcac caggtcgagg
      901 aaaggatcgt gcggcttggg caggtgccgg tgtacggcgt ccatcacctc ctgggtggcg
      961 tgcgggccgg tcacggccag caccgagggg tgcaggtgct tgatcaggtt ttcaggctca
     1021 ttccgggccc cagcgtccgc ccgatcagcg ccagcagcta tggtttttgc accaaggcag
     1081 ccggtgacga tgaccttgcc gttgaccgtc agggcctcgc cgatggtgtc caggctttcc
     1141 ttgaccgcct cgtcgatgaa cccgcaggtg ttgacgatga ccagatcggc accttcgaag
     1201 gtcttgctgg tctggtagcc ctcggccgac agctgggtca ggatcagctc ggagtcggtc
     1261 agcgccttgg ggcagcccag ggacacgaag ccgatgcgcg gcggcgtggt ggtttcactc
     1321 atgggggcga ttgtcgcagc cccgcgcgcc gaccgtaccg tcaaccgctg cgcttgatgc
     1381 cgaaggcacc cagcatgtgc tcggtctgcc ggctcatctg ctcctgcgcc tgcaggaaca
     1441 ggttcttgga ctgctccagg tagctgccca tcatgccctg catcatgggc gactgcatgc
     1501 gcatgaactg cgcccacaac tccgggctga ggccggcgga ctgctcggcc agccgggact
     1561 gaatctccat gaaggcctgg atgttctttt ccaggtagct gcccatgaag ccctgcatgg
     1621 catgaccgta gaagcggatg atgttggcca gcatgttctc ggtgaacatg ggggcgccgc
     1681 cggcttcttc ttccaggatg atctgcagca ggatgctgcg cgtcaggtcg tcgcccgtct
     1741 tggcgtcgcg caccacgaac ggctggtgcg ccatcaccag ctcgcgcacc tgggccagcg
     1801 tgatgtagga cgaggtggtg gtgtcgtaga gccgccggtt ggggtatttc ttgatcaccc
     1861 gctgcggcgc gccggccgac gcgtcgcgcg ccggctgggc gggagtcctg gctttgctgg
     1921 ggctcatggg ttcacgggtc gctgtaactg ctgcactgca acatattgta ggggcggcgg
     1981 cgccaccctg acccaaccac cgacccatgg ctttcccgca caccgacgct gcgcagcgct
     2041 cgccctgcgg gaagccagaa aaaggcttgc ggcggcgttg cgcctcttgc caaggccgcc
     2101 agcatgggct gcgagccgcg ccttgccgca agcctttttc tggcttccat agcaacgccg
     2161 catggcgcgg cgagcgcggt gcgggtgggt gcgcagcgtg ctcacccggg aggtgaaggc
     2221 catcgcagcc gccagacccc gcatcgatgc ggtctgcccg aagttgacaa gcggtcaacg
     2281 gccgcttcca ggttcaacca gcgcggcggc gcacaccctg ctaccatgcg cgccttcgcc
     2341 cgaagggagt cgtcaccatg tggaagctcg gtcgcctgct cacgctgttt cgcaaggagt
     2401 tgttgctcgc ctggggcttg ctgcgcaacc cgggcgcgcc gctgtcgtcc aagctggtga
     2461 cgctgctggc actgctgtac gtgatcagcc cggtcgatct ggtgtccgac ctgattccgg
     2521 tgctgggctg gctggacgat ggcctggtgg cctggctgct gtggcggctg gcgctgcgct
     2581 ttgcgccggc cgagctggtg gccacgctgc gcggcaagct cgacgcccgg ctgcgcccgg
     2641 cgccgcgctg aggctcagcg ctccttggtg tcgttgaagc gcccgtagct catcaggcgc
     2701 tcgtagcggc gctccagcag ttccttgacc ttcaggtcgc tgacctggcg ccaggcgtcg
     2761 cccagcgcac gcttgagcag cgtgcccatg tggcgcgggt cgcggtgcgc gccgccgacc
     2821 ggctcgctga cgatcttgtc gaccatgccc agcgccttca gccggtgcgc ggtgatgccc
     2881 agcgcttcgg cggcgtcctg cgccttgtcg ctggtcttcc acaggatgga ggcacagcct
     2941 tcgggactga tgaccgaata catggcgtac tgcagcatca gcacctgatc ggccacggcg
     3001 atggccagcg cgccgccgga gccgccttca ccgatgatgg tggtgatgat cggcacttcg
     3061 agctgcgcca t
//
LOCUS       DAOOOG010000243         3067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_38759_length_3067_cov_30.425299, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000243 DAOOOG010000000
VERSION     DAOOOG010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38759_length_3067_cov_30.425299"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1405)
                     /locus_tag="PK242_12705"
     CDS             complement(<1..1405)
                     /locus_tag="PK242_12705"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOZ94923.1"
                     /translation="MVSHAPFTPPVAFTDPAAALAQAQQIHDAGCAHLRAALAAYLAG
                     PAEPPAELARVRACYPFVRLRVPHGRHPDSRLAYGFVQRAGQYETTLTRPDLYADYYQ
                     EQFRLLIDNHGVTLEVGTSHQPIPLPFALAEDEHFEGQLSPAQRQRLPEVFDLPDLTA
                     MDDRIANGTHEVAPGAPEPLALFTAPRVDYSLHRLRHYTGTSPEHFQNFVLFTNYQFY
                     IDEFIALGEREMGRADSGYDSFVQPGNLVTRRAGLAPLPGDQRGAAPPRLPQMPAYHL
                     TRPDRGGISFVNIGVGPANAKTITDHIAVLRPHAWIMVGHCAGLRGSQQLGDYVLAHG
                     YVREDHVLDEDLPLWIPVPALAEVQIALQQAVADVTRLQGIELKRVLRTGTVATTDNR
                     NWELLGAGSPQRRFSQSRAIALDMESATIAANGFRFRVPYGTLLCVSDKPLHGEIKLP
                     GMANHFYRERVAQHLLIG"
     gene            1583..2656
                     /locus_tag="PK242_12710"
     CDS             1583..2656
                     /locus_tag="PK242_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOZ94924.1"
                     /translation="MNATDALNPLGHAASLGRLEDLPPEYVRAIHDLSVAPLWPQLRG
                     LLPRHQPVRHTQPFMWRYQDVRPHLLRAGELTPIEKAERRVLVLCNPGHGLDKLVATP
                     SIYVGLQLILPGEVAPNHRHTPTAVRFVIEGEGGYTTVNGEKCPMHPGDLILTPALNW
                     HEHGHEGSGPVVWLDALDLPFIYQMEASYAIEGPPQAAGAGPDASQNRYLRAGLLPYA
                     SLGQALRPQYPQIRFPWQDVRQALGALASVTARDEAVQLAYVNPETGRPCLPTLGFSA
                     QQLRAGQDFSPRRRSASSVIHVIEGRGESEIDGVTLKWTQGDTLAVPTHAQVTHRATG
                     RDAYLFHVDDAPMQRALGFYEDF"
     gene            2711..>3067
                     /locus_tag="PK242_12715"
     CDS             2711..>3067
                     /locus_tag="PK242_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOZ94925.1"
                     /translation="MTSYIFPPAPQAAVPVRGSPQQYAVSRIFCVGRNYAAHAREMGF
                     DPDREPPFYFNKTPIDLVPSGATVPYPLGSQNYHYEMELVIALGQPAFRVAPEQAQAC
                     VWGYACGLDMTRRDLQI"
BASE COUNT          459 a         1076 c         1082 g          450 t
ORIGIN      
        1 tgccgatcag caggtgctgg gccacgcgct cgcggtagaa gtggttggcc atgccgggca
       61 gcttgatctc gccgtgcagc ggtttgtcgc tcacgcacag cagggtgccg tagggcacgc
      121 ggaagcgaaa gccgttggcg gcgatggtgg cgctttccat gtccagcgcg atggcgcgcg
      181 actgcgagaa gcgccgctgc gggctgccgg cgcccagcag ctcccagttg cggttgtcgg
      241 tggtggccac ggtgccggtg cgcagcacgc gcttgagctc gatgccctgc agccgcgtga
      301 cgtcggccac cgcctgctgc agcgcgatct gcacctcggc cagcgccggc accgggatcc
      361 acagcggcag gtcttcgtcg agcacatggt cctcgcgcac gtagccgtgg gccagcacgt
      421 agtcgcccag ttgctggctg ccgcgcaggc cggcgcagtg gccgaccatg atccaggcgt
      481 gcgggcgcag cacggcgatg tggtcggtga tggtcttggc gttggccgga ccgacgccga
      541 tgttgacgaa gctgatgccc ccgcggtcgg ggcgcgtcag atggtaggcc ggcatctgcg
      601 gcaggcgcgg cggcgcggcg ccgcgctggt cgcccggcag cggcgccagg ccggcacgcc
      661 gcgtgaccag gttgccgggc tgcacgaagc tgtcgtagcc gctgtccgcc cggcccattt
      721 cgcgctcgcc cagggcgatg aattcgtcga tgtagaactg gtagttggtg aacagcacga
      781 agttctgaaa gtgctcgggc gaggtgccgg tgtagtgccg caggcggtgc agcgagtagt
      841 ccacgcgcgg cgcggtgaac agcgccagcg gttcgggcgc gccgggcgcc acctcgtgcg
      901 tgccgttggc gatgcggtcg tccatggcgg tcagatcggg caggtcgaac acctcgggca
      961 ggcgctggcg ctgcgccggg ctgagctggc cctcgaagtg ctcgtcctcg gccagcgcga
     1021 agggcaaggg gatcggctgg tggctggtgc ccacttccag cgtcacgccg tggttgtcga
     1081 tcagcaggcg gaactgctcc tggtagtagt cggcgtacag atcggggcgc gtcagcgtgg
     1141 tttcgtactg accggctcgc tgcacgaatc cgtacgccag gcgcgagtcg ggatgccggc
     1201 cgtgcggcac acgcagccgc acgaacgggt agcaggcacg cacgcgcgcc agctcggccg
     1261 gcggttcggc cgggccggca aggtaggccg ccagcgccgc acgcagatgg gcgcagccgg
     1321 cgtcgtggat ctgctgggcc tgggccaggg cggccgccgg gtcggtgaag gcaacggggg
     1381 gtgtgaaggg agcatgcgag accatggcgc attgtccatg gcctgtgtga ccgccgtgcg
     1441 tcatcgcggg cggcgccgca ccggcgtcgc ccaccgcgcc cccttgcatg ggcgaacgca
     1501 tgccgttaga atactaagca tacatattaa atccatcgcc cgggccgcac gcggcgcccg
     1561 gcctcacccc tggagacacc ccatgaacgc caccgacgcc ctgaatccgc tcggccacgc
     1621 cgcgtcgctg ggccggctgg aagacctgcc gcccgagtac gtgcgggcca tccacgacct
     1681 gtcggtggcg cccctgtggc cgcagctgcg cgggctgctg ccgcgccacc agccggtgcg
     1741 ccacacgcag cccttcatgt ggcgctacca ggacgtgcgc ccccacctgc tgcgcgccgg
     1801 cgagctgacg ccgatcgaga aggccgagcg ccgcgtgctg gtgctgtgca accccggcca
     1861 cgggctggac aagctggtgg ccacgccctc catctacgtc ggcctgcagc tcatcctgcc
     1921 gggcgaggtg gcgcccaacc accgccacac gccgacggcg gtgcgcttcg tcatcgaagg
     1981 cgaaggcggc tacaccacgg tgaacggcga aaaatgcccc atgcacccgg gcgacctgat
     2041 cctgacgccg gccctcaact ggcacgagca tggccacgag ggcagcggcc cggtggtgtg
     2101 gctggacgcg ctggatctgc ccttcatcta ccaaatggag gcctcctacg ccatcgaagg
     2161 cccgccgcag gcggcgggcg ccggaccgga cgcgtcgcag aacaggtacc tgcgcgccgg
     2221 cctgctgccc tatgcctcgc tcggtcaggc gctgcgcccg caatacccgc aaatccgctt
     2281 tccgtggcag gacgtgcgcc aggcgctggg cgcgctggcc agcgtgaccg cgcgcgacga
     2341 ggcggtgcag ctggcctacg tcaaccccga aaccggccgt ccctgcctgc cgacactggg
     2401 cttttcggcg cagcagctgc gcgccgggca ggatttttcg ccgcgcaggc gctcggccag
     2461 cagcgtgatc cacgtgatcg aaggccgcgg cgaatccgaa atcgacggcg tcaccctgaa
     2521 gtggacgcaa ggcgatacgc tggcggtgcc gacgcatgcc caagtcacgc accgcgccac
     2581 cggacgggac gcctacctgt tccacgtgga cgacgcgccg atgcagcgcg cgctcggttt
     2641 ttacgaggat ttttgacctc caacgcccgc cagtcgagcg ccagcagcta cattttcaag
     2701 agcacaccac atgacttcct acatcttccc ccccgccccc caggccgccg tgccggtgcg
     2761 cggcagcccg cagcagtacg ccgtcagccg catcttctgc gtcggccgca actacgccgc
     2821 ccacgcgcgc gagatggggt tcgaccccga ccgcgagccg cccttctact tcaacaagac
     2881 gcccatcgat ctggtgccga gcggtgccac cgtgccgtat ccgctgggca gccagaacta
     2941 ccactacgag atggagctgg tgatcgccct cggccagccg gccttccgtg tggcgcccga
     3001 gcaggcgcag gcctgcgtgt ggggctacgc ctgcgggctg gacatgacgc ggcgcgacct
     3061 gcagatc
//
LOCUS       DAOOOG010000244         3064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_38803_length_3064_cov_24.309405, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000244 DAOOOG010000000
VERSION     DAOOOG010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38803_length_3064_cov_24.309405"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="PK242_12720"
     CDS             <1..1344
                     /locus_tag="PK242_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ94926.1"
                     /translation="IDRGILERMTPAFEHLVRNCVVHGIEPQAERSARGKEATGLISI
                     QLQQAGNDVSVAFRDDGAGLNLSRIRERALQLGIVAADRELSDAEVANLVFMPGFSTA
                     SEVTELAGRGVGMDVVRSEVQALGGRIETQTTEGQGAAFRLVLPLTTAVTHVVMLRCG
                     ALAIGVPSNVVEIVQRASAKDLSRAYETGHYRYGDESLLFFWLGALLQDSPHSSEPPG
                     RTLPVVVLRSADQRVAVHVDEVLGNQEVVVKALGPQLSGLPGLVAITALASGAVALIY
                     NPVALASVYGVQARAMVELPQAAPAEVPVPQGPVPTPALAPSGIPLVLVVDDSITVRR
                     VTQRLLQREGYRVALAADGLQGLERLQAERPAVVLSDIEMPRMDGFDFVRNIRADEAL
                     RDLPVIMITSRIAEKHREHARELGVDHYLGKPYSEDELLGLIRNYTRAAAPVGAA"
     gene            1387..1986
                     /locus_tag="PK242_12725"
     CDS             1387..1986
                     /locus_tag="PK242_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOZ94927.1"
                     /translation="MADDAQPINLTNHFLIAMPGMVDGTFSRSVVYVCEHSQRGALGL
                     IINKPGDISLADLFERVDLPLSRPELGAQPVFQGGPLQTERGFVLHDPIVAEGLAPDQ
                     TVYASTLAVPGGLEMTTSKDVLEALSSGAGPRRVLVTLGYASWGEGQLESEIARNSWL
                     TVDADADLIFEAPIAQRYDLALRRLGLEAWMLSPDIGHA"
     gene            1983..2402
                     /gene="ruvX"
                     /locus_tag="PK242_12730"
     CDS             1983..2402
                     /gene="ruvX"
                     /locus_tag="PK242_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOZ94928.1"
                     /translation="MSASASAPEVPPHLGSFLAFDFGLKRTGVAVGNRLTRSATPQPT
                     IRAEGQARWAPLAARIAEWQPDALVVGVPCHPDGAAHDNTRRAQRFARQLRGRFGLPV
                     YEVDERYSTTEAHAAGARDADAGAAVVILEQFLRSLA"
     gene            2399..2899
                     /gene="pyrR"
                     /locus_tag="PK242_12735"
     CDS             2399..2899
                     /gene="pyrR"
                     /locus_tag="PK242_12735"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOZ94929.1"
                     /translation="MSALALDAEALYAELLRGVRSLVNPQTRLVGIVSGGRWLAERLQ
                     SDLGLAAPAGAISSAMHRDDFAKRGLAASGQTVLPFDVDGADILLLDDVLYTGRTLRA
                     VLNELFDYGRPQRVRLAVLVDRGGRQLPVAAEFAAARVALPADQSLELARSEDGRFSF
                     RVEQRG"
     gene            2896..>3064
                     /gene="pyrB"
                     /locus_tag="PK242_12740"
     CDS             2896..>3064
                     /gene="pyrB"
                     /locus_tag="PK242_12740"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332873.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOZ94930.1"
                     /translation="MTMLERRNPQLNRHGELIHLLSTEGLSRELLTHILDTAAQFVSV
                     SDREVKKVPLLR"
BASE COUNT          430 a         1059 c         1105 g          470 t
ORIGIN      
        1 atcgatcgcg gcatcctgga gcgcatgacg ccggccttcg agcacctggt gcgcaactgc
       61 gtggtgcatg gcatcgagcc acaggccgag cgcagcgcgc gtggcaagga agccaccgga
      121 ctgatcagca tccagctgca gcaggccggc aacgacgtgt cggtggcgtt ccgcgatgac
      181 ggcgccggtc tcaacctgtc gcgcatccgc gaacgcgctc tgcagttggg catcgtggcg
      241 gccgatcgtg aactgagcga tgccgaggtg gccaacctgg tgttcatgcc gggcttttcc
      301 accgccagcg aagtcactga actggccggt cgcggcgtcg gcatggacgt ggtgcgctcg
      361 gaggtccagg ccctgggcgg ccggatcgag acgcagacca ccgagggcca gggggcggcg
      421 ttccggctgg tgctgccgtt gaccaccgcc gtcacccacg tggtcatgct gcgctgcggc
      481 gcgctggcga tcggcgtgcc gtccaacgtg gtcgagatcg tgcagcgcgc atcggccaag
      541 gacctgtcgc gggcctacga gacggggcac taccgctatg gcgacgagtc gctgctgttc
      601 ttctggctgg gcgcgctgct gcaggattcg ccccacagca gcgagccgcc cgggcggacc
      661 ttgccggtgg tggtgctgcg ttcggccgat cagcgtgtgg cggtgcacgt cgacgaggtg
      721 ctgggcaacc aggaagtggt ggtcaaggcc ctggggcccc agctgtcggg cctgcctggc
      781 ctggtggcca tcaccgccct ggcttccggt gcggtggcgc tgatctacaa cccggtggcg
      841 ctggccagcg tgtacggcgt tcaggcacgc gcgatggtcg agctgccgca ggcggcgccc
      901 gccgaggtgc cggtgccgca agggccggtg ccgacacccg cactggcgcc ttcgggcatt
      961 cccctggtgc tggtggtcga cgactcgatc accgtgcgcc gcgtcaccca gcgcctgctg
     1021 cagcgcgaag gctaccgcgt ggccttggcc gccgatggtc tgcaggggct ggagcgcctg
     1081 caggccgagc ggccggcggt ggtgctgtcc gacatcgaga tgccgcgcat ggacggcttc
     1141 gacttcgtgc gcaacattcg tgccgacgaa gcgctgcgcg atctgccggt gatcatgatc
     1201 acctcgcgca tcgccgagaa gcaccgcgaa cacgcgcgcg aactgggcgt cgaccactac
     1261 ctcggcaagc cgtactccga ggacgaactg ctggggctga tccgcaacta cacccgcgcg
     1321 gcggctcccg tcggcgccgc ctgaggccac gggtgcggcc ccggcagcgg ccggtaaaat
     1381 cgctgcatgg ccgatgacgc gcagccgatc aacctgacga atcacttcct catcgccatg
     1441 ccgggcatgg tcgatgggac gttttcgcgc agcgtggtct acgtgtgcga gcacagccag
     1501 cgcggcgccc tgggcctgat catcaacaag ccgggcgaca tcagcctggc cgacctgttc
     1561 gagcgggtcg atctgcccct gtcgcgcccc gaactgggcg cgcagccggt gtttcagggc
     1621 ggccctttgc agaccgagcg cggcttcgtg ctgcacgacc cgatcgtggc cgagggtctg
     1681 gcgccggacc agacggtgta cgcctccacg ctggcggtgc ccggcgggct ggagatgacc
     1741 acgtccaagg acgtgctgga ggccctgtcc tccggtgccg ggccgcggcg cgtgctggtc
     1801 acgctgggct atgcgtcctg gggcgagggt cagctcgaat ccgagatcgc gcgcaacagc
     1861 tggctgacgg tggacgccga cgccgacctg atcttcgaag caccgatcgc gcagcgctac
     1921 gacctggccc tgcggcggct gggactggag gcgtggatgc tgtcaccgga catcggtcac
     1981 gcatgagcgc cagcgcgtcc gcgcccgagg tgccgcccca tctgggcagc tttctggcct
     2041 tcgacttcgg cctgaaacgc accggcgtgg cggtcggcaa ccggctcacg cgcagcgcca
     2101 cgccgcaacc caccatccgt gccgaaggcc aggcgcgctg ggcgccgctg gcggcgcgca
     2161 ttgccgaatg gcagcccgat gcgctggtgg tgggcgtgcc ctgtcacccc gacggcgcgg
     2221 cacacgacaa cacacgccgt gcgcagcgct tcgcgcgcca gctgcgtggg cgcttcgggc
     2281 tgccggtgta cgaggtggac gagcgctaca gcaccaccga ggcccatgcg gccggcgccc
     2341 gtgacgccga cgccggtgcg gcggtggtca tcctggagca gtttctgagg agtcttgcat
     2401 gagtgcactg gcgctggacg ccgaagccct gtacgccgag ctgctgcgcg gcgtgcggtc
     2461 cctggtcaac ccgcagacgc ggctggtcgg tatcgtctcg ggcgggcggt ggctggccga
     2521 gcgcctgcag tccgatctgg ggctggccgc ccccgcgggg gcgatttcct cggccatgca
     2581 ccgcgacgac ttcgccaagc gcggcctggc cgccagtggc cagaccgtgc tgccgttcga
     2641 cgtcgacggc gccgacatcc tgttgctcga cgacgtgctc tacaccggac gcaccctgcg
     2701 cgccgtgctc aacgagctgt tcgactacgg ccggccgcag cgcgtgcggc tggcggtgct
     2761 ggtcgaccgc ggcggccgcc agctgccggt ggcggccgaa ttcgccgccg cgcgggtggc
     2821 gttgccggcc gaccagtcgc tggagctggc gcgcagcgag gacggccgct tcagtttccg
     2881 tgtcgaacaa cgaggctgac catgctggag cggcgcaacc cccaactcaa ccgccacggc
     2941 gagctgatcc acctgctgtc caccgaaggg ctgtcgcgtg agctgctcac ccacatcctg
     3001 gacaccgcgg cgcagttcgt cagcgtcagc gaccgcgagg tcaagaaggt gccgctgctg
     3061 cgcg
//
LOCUS       DAOOOG010000245         3045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_39128_length_3045_cov_26.490635, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000245 DAOOOG010000000
VERSION     DAOOOG010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39128_length_3045_cov_26.490635"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PK242_12745"
     CDS             <1..585
                     /locus_tag="PK242_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOZ94931.1"
                     /translation="RPIEVDVRFIAATNRILLGEVKAGLFRDDLYYRLQVVAINIPPL
                     RERGDDIFVLARASLEVFNRLHGSRMTSFEPEVEDVFRRYAWPGNVRELENLLERICL
                     LEDGERVRLEHLPDRIVRQARGQPAAPAAAEVAPSDYHAATERFQRQLIDGALRTAGQ
                     NLAEAARALGLSRHALRHQMKKLGQDPEASTETA"
     gene            745..2106
                     /locus_tag="PK242_12750"
     CDS             745..2106
                     /locus_tag="PK242_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HOZ94932.1"
                     /translation="MDSLKIICPNGHLGFAPLRVESFELGVAARPDYIAADSGSDDVG
                     PVPLGSDTSTSPESWQRHDLEHMLLAARKLGVPMVIGSAGDTGSNSRVDRYVAIIQEL
                     ARQHGLPRFKLGYFYSEVNPQALRARMAQGAVVRGLDGFADLTPAELDATDRIVAMAG
                     VHPYLRLLEQGADVIIGGRSSDAAVFAAPALHHGFAADHAYYLGKVLECASFCAEPYG
                     GKETVMGEISRQDVRATAMHPQQRCTIASVSGHAMYERSNPYEEFFAGGKLDMSDCHY
                     EQISERTTRITGSRFLPDERVRVKLEGAGKVGERYVGLCGIRDPYTIAHVDAVIGWAR
                     EQVKARFGTTGYELHYNVYGRDGVMGALEPLRDQPGHELCIMVQGVAPTREMAEEVTM
                     IGLRQMFYARLPDVKGTAGSVAFPLDEVLHASPAYRWTLNHTIEVDDPMALFSTHLVE
                     AGI"
     gene            2121..2441
                     /locus_tag="PK242_12755"
     CDS             2121..2441
                     /locus_tag="PK242_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HOZ94933.1"
                     /translation="MNTTPLSQLAKTIRSKNAGVNKITFDIIFRERAEYERVKRSGAL
                     TRASMAALYRIDPARISDFVEYEPGLAIKFTIYRERPSGSAGDGDIFGAQQYAPLLDL
                     PIPA"
     gene            2477..>3045
                     /locus_tag="PK242_12760"
     CDS             2477..>3045
                     /locus_tag="PK242_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOZ94934.1"
                     /translation="MPRFIARRALLIASAILPMLALAQTDAEPKLPAMMKIVVPFGAG
                     ASNDAIARAVAPLLARRLGNTVIVENKPGAAGVIGSDYVAKSPADGSVLLLSSSTFVT
                     AAVTQPRLPYDPLTAFVPVSMVGNGPMLLAVSAATSIKSAAELVAAAKAKPGSLTYGT
                     SGTGSIAHLASEMLSDAAGIRMTHVPYKGA"
BASE COUNT          495 a         1060 c         1032 g          458 t
ORIGIN      
        1 cgcccgatcg aggtggacgt gcgcttcatc gccgccacca accgcatcct gctgggcgag
       61 gtcaaggccg ggctgttccg cgacgacctg tactaccggc tgcaggtggt cgccatcaac
      121 atcccgccgc tgcgcgagcg cggcgacgac atcttcgtgc tggcccgggc gtcgctggag
      181 gtcttcaacc gcctgcatgg cagccggatg acgagcttcg agcccgaggt cgaggacgtt
      241 tttcgccgct acgcctggcc cggcaacgtg cgcgagctgg aaaacctgct ggagcgcatc
      301 tgcctgctgg aggacggcga gcgcgtgcgg ctggagcacc tgcccgaccg catcgtgcgc
      361 caggcccgtg ggcagccagc agcgcccgcg gccgccgagg ttgcgccatc cgactaccac
      421 gcagccacgg agcgcttcca gcgccagctc atcgacggcg cgctgcgaac ggcgggacag
      481 aacctggccg aggcggcccg cgcgctgggg ctcagccgcc acgcactgcg ccaccagatg
      541 aagaagctcg gtcaggatcc cgaggcgtcg actgaaactg cgtgaaaatc acgcgtgcgc
      601 gtggaatgcg cgcattttca tcgtcgacgc ggaaggcgac cgcatggcaa gctgttgatt
      661 tgcaaggaat tcgtcccccg gccggggctg gcacgcttgc tgcatgactg gcgaggtatt
      721 cacgaccttc gcaaggaaag caggatggat tcgctcaaga tcatctgccc caacgggcac
      781 ctggggtttg cgccgctgcg cgtggaaagc ttcgagctgg gggtggcggc gcggcccgac
      841 tacatcgcgg ccgactctgg cagcgacgat gtggggccgg tgccgctggg ctcggacacc
      901 tcgaccagcc ccgaatcctg gcagcggcac gacctggagc acatgctgct ggccgcccgc
      961 aagctgggcg tgccgatggt catcggctcg gccggcgaca ccggcagcaa cagccgcgtg
     1021 gaccggtacg tggccatcat ccaggagctg gcgcgccagc acggactgcc ccgcttcaag
     1081 ctgggctact tctattccga ggtcaacccc caggcgctgc gcgcgcgcat ggctcagggc
     1141 gccgtggtgc gcggactgga cggctttgcc gacctgacgc cggccgagtt ggacgccacc
     1201 gaccgcatcg tggccatggc gggcgtgcac ccgtacctgc ggctgctgga gcagggggcc
     1261 gacgtcatca tcggcgggcg cagctccgac gcggccgtgt tcgccgcgcc ggcgctgcac
     1321 cacggcttcg cggccgacca cgcctactac ctgggcaagg tgctggagtg cgcgtccttc
     1381 tgcgccgagc cgtatggcgg caaggaaacg gtgatgggcg agatctcgcg ccaggacgtg
     1441 cgcgccaccg ccatgcaccc gcagcagcgc tgcaccatcg cgtcggtgtc gggccacgcg
     1501 atgtacgagc gctccaaccc ctacgaggag ttctttgccg gcggcaagct ggacatgagc
     1561 gactgccatt acgagcagat cagcgagcgc accacccgca tcaccggctc gcgcttcctg
     1621 ccggacgagc gcgtgcgcgt caagctggag ggcgcgggca aggtcggcga gcgctacgtg
     1681 ggcctgtgcg gcatccgcga cccctacacc atcgcccatg tggatgccgt catcggctgg
     1741 gcgcgcgagc aggtgaaggc gcgcttcggc acgacgggct acgagctgca ctacaacgtc
     1801 tacggccgcg acggcgtcat gggcgcgctg gagccgctgc gcgaccagcc agggcacgag
     1861 ctgtgcatca tggtgcaggg cgtggcgccg acgcgcgaga tggccgagga agtcaccatg
     1921 atcggcctgc gtcagatgtt ctacgcccgc ctgcccgacg tgaagggcac cgccggctcg
     1981 gtggcgttcc cgctggacga ggtgctgcac gccagcccgg cgtatcgctg gacgctcaac
     2041 cacacgatcg aggtcgatga cccgatggcg cttttctcga cccacctggt cgaggctgga
     2101 atctgattgg agagcctgtc atgaacacca cccccctgag tcagctggcc aagaccatcc
     2161 gcagcaagaa cgcgggcgtc aacaagatca ccttcgacat catcttccgc gagcgtgccg
     2221 agtacgagcg cgtcaagcgc tcgggcgcgc tgacccgcgc gtcgatggcg gcgctgtacc
     2281 gcatcgatcc ggcgcgcatc agcgacttcg tcgagtacga gccgggcctg gcgatcaagt
     2341 tcaccatcta ccgagagcgc cccagcggca gcgccggcga cggcgacatc tttggcgccc
     2401 agcagtacgc gccgctgctg gatctgccga ttccggcttg aggcctttca cccatcacca
     2461 cccacaggag acactcatgc cacgcttcat tgctcgtcgc gcgctgctca tcgcctcggc
     2521 catcctgccc atgctcgcgc tggcgcagac cgatgccgag ccgaagttgc ccgccatgat
     2581 gaaaatcgtc gtgccgttcg gcgccggcgc cagcaacgat gccatcgcgc gcgcggtggc
     2641 gccgctgctg gccaggcgac tgggcaacac ggtcatcgtc gagaacaagc ccggcgccgc
     2701 cggcgtgatt ggttccgatt acgtggccaa gagcccggcc gacggctcgg tgttgctgct
     2761 gagttcgtcc accttcgtga ccgcggccgt cacccagccc aggctgccct acgacccgct
     2821 gaccgcgttc gtgccggtgt ccatggtggg caatgggccg atgctgctgg cggtgagcgc
     2881 cgccacgtcg atcaagtcgg cggcggagct ggtggccgcg gccaaggcca agcccggcag
     2941 cctgacctac ggcacctcgg gcaccggctc catcgcccac ctggccagcg aaatgctgtc
     3001 ggacgcggcg ggcatcagga tgacgcacgt gccctacaag ggcgc
//
LOCUS       DAOOOG010000246         3030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_39401_length_3030_cov_43.576471, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000246 DAOOOG010000000
VERSION     DAOOOG010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39401_length_3030_cov_43.576471"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK242_12765"
     CDS             <1..514
                     /locus_tag="PK242_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94935.1"
                     /translation="LAVALGAAACTPTLDWREVRLAPSPAIALLPCKPDRTTRDVPLG
                     GVPTALSVAGCDAGGATFALMTAQLPAGRDPDAVLAGWQQATLAHMRAQGTPERRPFT
                     PPGARALPHAQRLTARGQRPDGRPVMAQAVWSAQPAAGGGTELLHAVVYAERLRPEAA
                     DAFFDAIRWP"
     gene            511..1770
                     /locus_tag="PK242_12770"
     CDS             511..1770
                     /locus_tag="PK242_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ94936.1"
                     /translation="MSAGAELLPRRTAVLVFLAFAGAYFLSALLRAVTATLSPVLTAD
                     FALHARDLGLLAGGYFLGFAAMQLPLGAWLDRYGPRRVSVALLAVAVLGCAVFALAQG
                     FAGLLAARVLMGMGVAAGLMAPLTGYRRWFDAAMQLRANAWMLMTGSLGMLASTLPVQ
                     WLLPLLGWRPLFWGLALLGLLAMAVIALRVPGWRPPDATNSIADGADRAGAGGRNGSY
                     AQIVRQAEFRRAWPLGMVSFGGMVAIQSLWAGPWLTRVVGRSPLEAATGLFWINASML
                     LTFWAWGLLLPRLTARGIGIDAIVARVQPFSFVALAALVAAGPAVGGWGVALLALYCV
                     LSTPSAQVQPVLALAYPPQVAGRALSAFNLVIFVGIFLVQWGIGLGADALAALGWSEA
                     DALRGALGAFGLLSAACWLHHLRAGRR"
     gene            1782..2190
                     /locus_tag="PK242_12775"
                     /pseudo
     CDS             1782..2190
                     /locus_tag="PK242_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            2201..2470
                     /locus_tag="PK242_12780"
     CDS             2201..2470
                     /locus_tag="PK242_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOZ94937.1"
                     /translation="MIKTDITISNKLGLHARAAAKLTKLAAAYPCEVWMSKGERRINA
                     KSIMGVMMLAAGIGSVVTLETDGAQEQEAMDALRALIDDKFGEGQ"
     gene            2467..>3030
                     /locus_tag="PK242_12785"
     CDS             2467..>3030
                     /locus_tag="PK242_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HOZ94938.1"
                     /translation="MSFSIHGISVTRGIAIGRAVLVASSLVDVAHYFIAPEQVEAEIA
                     RLRAARNAVVEELQRMQADMPRDLPPELGAMLDVHLMLLQDEALTSEIRRWITERLYN
                     AEWALASQLEVLIRQFDEMDDPYLRERKADLEQVVERVLRHLKGGPAPIAAPAPRAGR
                     QQELGLGDIDVPLVLVAHDLSPADMLQF"
BASE COUNT          347 a         1109 c         1128 g          446 t
ORIGIN      
        1 gctggcggtg gccctgggcg cggcggcctg cacgcccacg ctggactggc gcgaagtgcg
       61 cctggcgcct tcgccggcca ttgccttgct gccctgcaaa cccgaccgca ccacccgcga
      121 cgtgccgctg ggaggcgtgc cgaccgcgct cagcgtggcc ggttgcgacg ccggcggcgc
      181 caccttcgcc ctgatgacgg cccagctgcc ggccgggcgc gaccccgacg ccgtgctggc
      241 cggctggcag caggccacgc tggcccacat gcgcgcccag ggcacgcccg agcggcgccc
      301 gttcacgccg cccggcgccc gcgccctgcc ccatgcccag cgcctgaccg cccgcggcca
      361 gcggcccgac ggccggccgg tgatggcgca ggcggtgtgg agcgcgcagc cggccgccgg
      421 cggcggcacc gagttgctgc acgccgtggt ctacgccgag cgcctgcgcc ccgaggcggc
      481 cgacgccttc ttcgacgcca tccgctggcc atgagcgcgg gtgccgagct gttgccgcgc
      541 cgcaccgcgg tgctggtgtt tctggcgttt gccggcgctt actttctgtc ggcgctgctg
      601 cgcgccgtca ccgccacgct gtcgccggtg ctgaccgccg acttcgcgct gcacgcgcgc
      661 gacctcggtc tgctggcggg cggctacttt ctgggctttg ccgccatgca gctgccgctg
      721 ggcgcctggc tggaccgcta cgggccgcgc cgcgtcagcg tggcgctgct ggcggtggcc
      781 gtgctgggct gtgccgtgtt cgcgctggcg cagggctttg ccggcctgct ggcggcgcgc
      841 gtgctgatgg gcatgggcgt ggccgccgga ctgatggcgc cgctgaccgg ctaccgccgc
      901 tggttcgacg ccgccatgca gctgcgcgcc aacgcctgga tgctgatgac cggctcgctc
      961 ggcatgctgg cgtccaccct gccggtgcag tggctgctgc cgctgctggg ctggcggccg
     1021 ctgttctggg ggctggcgct gctggggctg ctggcgatgg cggtgatcgc actgcgggtg
     1081 cccggctggc gccccccgga tgctaccaac agcatagctg atggcgcaga tcgggcgggc
     1141 gctggaggcc ggaatggctc ttacgcccag atcgtgcggc aggccgaatt ccggcgcgcc
     1201 tggccgctcg gcatggtcag tttcggcggc atggtggcga tccagtcgct gtgggccggg
     1261 ccgtggctga cccgcgtggt ggggcgcagc ccgctggaag cggccaccgg cctgttctgg
     1321 atcaacgcca gcatgctgct gaccttctgg gcctggggcc tgctgctgcc gcggctgacg
     1381 gcgcgcggca tcggcatcga cgccatcgtg gcgcgcgtgc agccgttcag cttcgtggcg
     1441 ctggcggccc tggtggcggc cggaccggcg gtgggcggct ggggcgtggc gctgctggcg
     1501 ctgtactgcg tgctgagcac cccgtcggcg caggtgcagc cggtgctggc gctggcctac
     1561 ccgccgcaag tggccgggcg cgcgctgtcg gccttcaacc tggtgatctt cgtcggcatc
     1621 ttcctggtgc agtggggcat cggcctgggc gccgacgcgc tggccgccct gggctggtcc
     1681 gaggccgacg cgctgcgcgg cgcgctgggc gccttcgggc tgctcagcgc cgcctgctgg
     1741 ctgcaccacc tgcgggccgg gcgccgataa tccagcctgc catgagcaac gccgttctgc
     1801 tgatcgccca cgccccgctg gcgcacgccc tgcgcgaatg cgcgctgcac gtgtttgccg
     1861 aagccgccgc cgacatcgga gcgctcgacg tccaggccca cgaaccgccc gagcagaccc
     1921 tggcgcgcgc ccaggcgctg tgcgccggct ggggcgagcg cccggtgctg gtgctgaccg
     1981 acgtgctggg cgccaccccc agcaacgtgg cgcggcgcct gatcgacggc cggcgcatgc
     2041 ggctgctggc cggcgtcaac ctgccgatgc tgctgcgcgc gctcacctac cgcgccgagc
     2101 cgctggccga catggcgcag cgcgcgctgg acggcggcat gcgcggcgtg gtggtggtgg
     2161 agccgcccgt cgtcaccgaa cccgcgctga agggcgccgc atgatcaaga ccgacatcac
     2221 catcagcaac aagctgggcc tgcacgcccg cgccgccgcc aagctgacca agctggccgc
     2281 cgcctacccc tgcgaagtct ggatgagcaa gggcgagcgc cgcatcaacg ccaagagcat
     2341 catgggcgtg atgatgctgg ccgccggcat cggcagcgtg gtcacgctgg agaccgacgg
     2401 cgcgcaggag caggaggcga tggacgcgct gcgcgcactg atcgacgaca agttcggcga
     2461 ggggcaatga gcttttccat ccacggcatc agcgtcaccc gcggcatcgc catcgggcgt
     2521 gccgtgctgg tggcctccag cctggtggac gtggcgcact acttcatcgc gcccgagcag
     2581 gtcgaggccg aaatcgcccg cctgcgcgcc gctcgcaacg ccgtggtcga agagctgcag
     2641 cgcatgcagg ccgacatgcc gcgcgacctg ccgcccgagc tgggcgccat gctcgacgtg
     2701 cacctgatgc tgttgcagga cgaggcgctg acgtccgaga tccgccgctg gatcaccgag
     2761 cgcctgtaca acgccgaatg ggcgctggcc agccagctgg aggtgctgat ccgtcagttc
     2821 gacgagatgg acgaccccta cctgcgcgag cgcaaggccg acctcgagca ggtggtcgag
     2881 cgcgtgctgc gccacctcaa gggcggcccc gccccgatcg ccgcgccggc gccgcgcgcc
     2941 ggtcgccagc aggagctggg cctgggcgac atcgacgtgc cgctggtgct ggtggcgcac
     3001 gacctgtcgc cggccgacat gctgcagttc
//
LOCUS       DAOOOG010000247         3026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_39467_length_3026_cov_26.763043, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000247 DAOOOG010000000
VERSION     DAOOOG010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39467_length_3026_cov_26.763043"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PK242_12790"
     CDS             <1..275
                     /locus_tag="PK242_12790"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOZ94939.1"
                     /translation="AAAQRLVAARERGHFKDVGSALALVPLPGGGSADTSGVAVGTQF
                     FEVRGRLRLDQLALEEVSTVQRDPSTRQVRTLWRERRALGTPAAGG"
     gene            304..1521
                     /gene="gspL"
                     /locus_tag="PK242_12795"
     CDS             304..1521
                     /gene="gspL"
                     /locus_tag="PK242_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HOZ94940.1"
                     /translation="MSLLLIALPPGPPADYAFATSADGQGVSAHGSAAPALLPAAGRG
                     VEVVAVVPAARLSWQAVQLPRGVGPGAPRLRSILEGLLEEQLLDEPARLHLALAPGAQ
                     GGARAWVAVCERAWLAAHLAALDAAGRPPARIVPELSPQHGPTRLWLSGEPERPWLLM
                     SGDGVPGGAQALPFGTGSAALLPAAAADAPAPELLAEPALAALAEQAFQRPVAIRSAA
                     ERLLHASRSPWDLAQLEFSRGGRARAARRAGTLWRDFLHAPAWRPARWALALLLLVNL
                     AGLNLLAWRTGQALAAQRQAQQALLTDTFPQVKVVVDAPVQMAREVAALRQATGAASG
                     RDLEPMLQAFGQLALTQQAPTAIEFEAGELRLKGLTLAAGALAEANPRLRPLGLHLRT
                     EGDTAVLRQEGAP"
     gene            1580..2104
                     /gene="gspM"
                     /locus_tag="PK242_12800"
     CDS             1580..2104
                     /gene="gspM"
                     /locus_tag="PK242_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOZ94941.1"
                     /translation="MSAPASAAAPTRGPRALGAAWARLAARERRLVLLAAAVLGLALL
                     WLLAVAPALRTLRQAEAQRATLAAQTEQLQRLRAEAQALQALPRLGHDEALRALESSV
                     RQRLGDSAQLSVVGERATLVLRQTSAEALAQWLADARANARANTVEARLTRAGDNAPG
                     APVRWNGTLTLALP"
     gene            2101..2922
                     /gene="gspN"
                     /locus_tag="PK242_12805"
     CDS             2101..2922
                     /gene="gspN"
                     /locus_tag="PK242_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HOZ94942.1"
                     /translation="MKRLAPAPRPAGAAAPPPRAPWGWALVGALLGLLLVGLLAAPAR
                     WLAAGVARASGDMVQLTDAQGSLWGGSARLLLTGGAGSQDRSALPGRVHWRLRPGWGA
                     LALQLRADCCTPSGPLGLRLSPRWGGAALALADAHSVWPAALLAGLGTPFNTLQPQGE
                     LNLTTQALSLHWQAGRASLQGGAELTLRQLSSRVATVQPLGSYRVQLQGGDTPTLTLS
                     TLEGPLRLTGSGQWVGRRLRYSGEARADPGLEAALANLLNLLGRRQGDRALMSFG"
BASE COUNT          319 a         1229 c         1144 g          334 t
ORIGIN      
        1 tggccgccgc ccagcgcctg gtggcggcgc gcgagcgcgg ccacttcaag gacgtcggca
       61 gcgcgctggc gctggtgccg ctgcccggag gcggcagcgc cgacaccagc ggcgtggcgg
      121 tgggcacgca gttcttcgaa gtgcgcggcc ggctgcggct ggaccagctg gcgctggaag
      181 aagtctccac cgtgcagcgc gaccccagca cgcgccaggt gcgcaccctg tggcgcgagc
      241 gccgcgcgct cggcacaccc gccgccggcg gctgaacgcg gcacctacaa tgccgcgacc
      301 cccatgagcc tgctgctgat cgccctgccg ccgggcccgc cggccgacta cgccttcgcc
      361 acctcggccg acggccaggg cgtgagcgcg cacggcagcg ccgcgccggc cctgctgccg
      421 gccgccgggc gcggcgtcga agtggtggcg gtggtgccgg cggcgcggct gtcgtggcag
      481 gcggtgcagc tgccgcgcgg cgtcggcccc ggcgccccgc gcctgcgcag catcctggaa
      541 ggcctgctgg aagagcagct gctcgacgag ccggcccgcc tgcacctggc gctcgccccc
      601 ggcgcccagg gcggcgcccg cgcctgggtg gcggtgtgcg agcgcgcctg gctggccgcc
      661 cacctggcgg cgctcgatgc cgccggccgc ccgcccgccc gcatcgtgcc cgaactcagc
      721 ccccagcacg gccccacccg cctgtggctg agcggcgagc ccgaacgccc ctggctgctg
      781 atgagcggcg acggcgtgcc cggcggcgcc caggcgctgc ccttcggcac cggcagcgcc
      841 gccctgttgc ccgccgcggc ggccgacgcc ccggcgcccg agctgctggc cgagccggcg
      901 ctggcggcgc tggccgaaca ggcgttccag cgcccggtgg ccatccgctc ggcggccgag
      961 cgcctgctgc acgccagccg cagcccctgg gacctggcgc agctcgaatt cagccgcggc
     1021 ggccgcgccc gcgcggcgcg gcgcgccggc accctgtggc gcgacttcct gcacgccccc
     1081 gcctggcggc cggcgcgctg ggcgctggcg ctgctgctgc tggtcaacct ggccggcctc
     1141 aacctgctgg cgtggcgcac cggccaggcg ctggcggcgc agcgccaggc ccagcaggcg
     1201 ctgctgaccg acaccttccc gcaggtcaag gtggtggtcg acgcgcccgt gcagatggcg
     1261 cgcgaagtgg ccgccctgcg ccaggccacc ggcgccgcct cgggccgcga cctggaaccg
     1321 atgttgcagg cttttgggca gctggcgctt acccagcaag cgccgacagc tatcgaattt
     1381 gaagcgggcg agctgcgcct gaaaggcctc acgctggccg ccggcgcgct ggccgaagcc
     1441 aacccgaggc tgcgcccgct gggcctgcac ctgcggaccg aaggcgacac cgcggtgctg
     1501 cggcaggagg gcgcaccatg agcgcgacgg gccgttccca agcgcgaatc ccgccgcgca
     1561 acgcacggag gggcagcgca tgagcgcgcc agccagcgcc gcggcgccca cgcgcggccc
     1621 gcgcgccctg ggcgcggcct gggcgcgcct ggccgcgcgc gagcgccgcc tggtgctgct
     1681 ggccgccgcc gtgctgggcc tggccctgtt gtggctgctg gccgtcgcgc cggcgctgcg
     1741 cacgctgcgc caggccgagg cgcagcgcgc cacgctggcg gcgcagaccg agcagctgca
     1801 gcggctgcgc gccgaagcgc aggcgctgca ggcgctgccc cgcctgggcc atgacgaggc
     1861 gctgcgcgcc ctcgaaagct cggtgcgcca gcgcctgggc gacagcgccc agctcagcgt
     1921 ggtgggcgag cgcgccaccc tggtgctgcg ccagaccagc gccgaggcgc tggcgcagtg
     1981 gctggccgac gcccgcgcca acgcccgcgc caacacggtc gaagcgcggc tcacgcgcgc
     2041 cggcgacaac gctcccggcg cgccggtgcg ctggaacggc acgctgacgc tggcgctgcc
     2101 atgaagcgcc tcgcgcccgc gccgcgccct gccggcgccg ccgcgccgcc gccccgcgcg
     2161 ccctggggct gggcgctggt cggggcgctg ctgggcctgc tgctggtcgg gctgctggcg
     2221 gcgccggcgc gctggctggc cgccggcgtc gcccgcgcca gcggcgacat ggtgcaactg
     2281 accgacgccc agggcagcct gtggggcggc tcggcgcgcc tgctgctgac cggcggcgcc
     2341 ggcagccagg accgcagcgc cctgcccggc cgcgtgcact ggcgcctgcg gccgggttgg
     2401 ggggcgctgg cgctgcaact gcgcgccgac tgctgcaccc cgtccggccc gctgggcctg
     2461 cgcctgagcc cgcgctgggg cggcgccgcg ctggcgctgg ccgacgccca cagcgtctgg
     2521 ccggcggccc tgctggccgg cctgggcaca ccgttcaaca ccctgcagcc gcagggcgag
     2581 ctgaacctga ccacgcaggc gctgtcgctg cactggcagg ccggccgcgc cagcctgcag
     2641 ggcggcgccg aactgacgct gcgccagctg tcctcgcgcg tggccaccgt gcaacccctg
     2701 ggttcgtacc gcgtgcagct gcagggcggc gatacgccga cgctgaccct gtccacgctg
     2761 gaaggcccgc tgcgcctgac cggctccggc caatgggtgg gccgccgcct gcgctacagc
     2821 ggcgaagcgc gcgccgaccc cggcctggaa gcggcgctgg ccaacctgct caacctgctc
     2881 ggccgccgcc agggcgatcg ggccctgatg tccttcggct gagcggcgcc tgtccgcgcc
     2941 gccatttgct acagtgcaac acgctttgcc tccgaccccg cccatgccac gcctgtcccg
     3001 ccgacccttg ccgtccggcg cgcgcc
//
LOCUS       DAOOOG010000248         3025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_39488_length_3025_cov_23.054545, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000248 DAOOOG010000000
VERSION     DAOOOG010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39488_length_3025_cov_23.054545"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /locus_tag="PK242_12810"
     CDS             <1..119
                     /locus_tag="PK242_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461028.1"
                     /note="with YejAEF is involved in resistance to microcin
                     C; Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="microcin ABC transporter permease"
                     /protein_id="HOZ94943.1"
                     /translation="RDYPVVLGTLYLFTLIGLVTKLISDLTYVWVDPRVKFD"
     gene            124..1158
                     /locus_tag="PK242_12815"
     CDS             124..1158
                     /locus_tag="PK242_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ94944.1"
                     /translation="MVTASVSPARRAWRRFRRNRLGLWSLVLFGVLVGFSLLAELVSN
                     DKPLLVRYQGQWYVPLVKTYPEKVFGGDFETETDYLDPFIRQQFSQPGNWAIYPPNPY
                     GARTINYFAKAPNPSAPSRDNWLGTDDRGRDLLAQLIYGFRVSVLFALALTAVGVLLG
                     VAAGAVQGFFGGRIDLAFQRFIEIWGSMPELYLLIIFSAILAPSLGLLLVLLSLFGWM
                     GLSDYVRAEFLRNRQLDYVKAARALGVSSGQIIWRHILPNSMTPVVTFLPFRMSAAIL
                     ALTSLDFLGLGVPPGTPSLGELLSQGKNNIDAWWISISTFGVLVLTLLLLTFMGDALR
                     DALDPRKAQS"
     gene            1282..2901
                     /locus_tag="PK242_12820"
     CDS             1282..2901
                     /locus_tag="PK242_12820"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOZ94945.1"
                     /translation="MAEAAPALLEVRDLSVAFGAKTVVEGVSLAIAPGEKLALVGESG
                     SGKTVTALSLLRLLHDARVGGSAWFDDGRQRRELLALPERELRGLRGDQIAMIFQEPM
                     TALNPLMTVGAQIAEVLRLKRALTPAQSAQAAIELIAHTGIPEPERRAGAYPHQLSGG
                     QRQRAMIAMALACAPKLLLADEPTTALDVSLRGQMLELLDDLRRDHGMAVLLITHDLN
                     LVRRFADRVAVMQHGRLVEQGATESIFNAPEHPYTRQLMASRPERDVVEAAADAGATP
                     ALSAQGLRVVYPTPLPGIRGWFRKGAFVALQGADFAIAAGRTLGVIGESGSGKSTLAQ
                     AALGLLPRPQVAGQVQVSGRSWQGRAGADRPLRRAVQVVFQDPFSSLSPRLTVEEIVG
                     EGLRVHAPVMPPAERSQRVGQALADVGLTQAAFPGLLGRYPHEFSGGQRQRIALARAL
                     IVQPELLVLDEPTSALDVTIQKQVLGLLQQLQRERGLAYLLITHDVDVVRAMAHEVLV
                     LKDGAVVEAGAAAQVLSQPQHPYTQRLLAAA"
BASE COUNT          450 a         1029 c         1042 g          504 t
ORIGIN      
        1 ggcgcgacta cccggtggtg ctgggcacgc tgtacctgtt caccctgatc ggccttgtga
       61 ccaagctgat cagtgacctg acctacgtgt gggtggatcc gcgtgtcaag ttcgactgag
      121 ggcatggtca ccgcatcggt ttcgccggcg cgccgcgcct ggcggcgctt tcggcgcaac
      181 cgcctgggct tgtggagcct ggtgctgttt ggtgtcctgg tggggttcag cctgctggcc
      241 gagctggtgt ccaacgacaa gccgctgctg gtgcgctacc agggacagtg gtacgtgcca
      301 ctggtcaaga cctacccgga aaaggtgttc ggcggcgact tcgagaccga gaccgactac
      361 ctcgacccct tcatccgcca gcagttcagt cagcccggca actgggccat ctacccgccc
      421 aacccgtatg gcgccagaac catcaactac tttgccaagg cacccaaccc cagcgccccc
      481 agccgcgaca actggctggg caccgacgac cgcgggcgcg atctgctggc gcagctcatc
      541 tacggctttc gcgtcagcgt gctgttcgcc ctggcgctga cggcggtcgg cgtgctgctg
      601 ggcgtggcgg cgggggcggt gcagggcttt ttcggcggca ggatcgatct ggcgtttcag
      661 cgcttcatcg agatctgggg ctccatgccc gagctgtacc tgctgatcat cttctcggcc
      721 atcctggcgc ccagcctggg cctgctgctg gtgctgctca gcctgttcgg ctggatggga
      781 ctgtcggact acgtgcgcgc cgagttcctg cgcaaccgcc agctcgacta cgtcaaggcg
      841 gcgcgagcgc tgggcgtgtc cagcgggcag atcatctggc gccacatcct gcccaacagc
      901 atgacgccgg tggtcacctt tctgccgttt cgcatgagcg cggccatcct ggcgctgacc
      961 tcgctggact tcctgggcct gggcgtgccg ccgggcacgc ccagcctggg cgagctgctc
     1021 agtcagggca agaacaacat cgacgcctgg tggatatcga tctccacctt cggcgtgctg
     1081 gtgctgacgc tgctgctgct gaccttcatg ggcgacgcgc tgcgcgacgc gctcgatccg
     1141 aggaaggcgc agtcatgaac aacccacgcg cgtacgcaga cgacgccaaa gcggcgcgaa
     1201 gggcgcagaa cgaagccagg caatgggatc gttctgcgtc ttctgcgaat tctttgcgtc
     1261 ctctgcgtat gaaggttcaa catggctgaa gccgccccag cgctgctcga ggtgcgcgac
     1321 ctcagtgtcg ccttcggcgc caagaccgtg gtcgagggcg tgagcctggc catcgcgccg
     1381 ggcgagaagc tggcgctggt gggcgaatcg ggctcgggca agacggtgac ggcgctgtcg
     1441 ctgctgcgtc tgctgcacga cgcacgggtc ggcggcagcg catggttcga cgacggtcgg
     1501 cagcggcgcg agctgctggc actgcccgag cgcgaattgc gcggcctgcg cggcgaccag
     1561 atcgcgatga tttttcagga accgatgacg gcgctcaacc cgctcatgac cgtgggcgcg
     1621 cagattgccg aggttctgag gttgaaaagg gctctaacgc ccgcccaatc tgcgcaagca
     1681 gctattgagt tgatagcgca taccggcatt cccgaacctg agcggcgcgc cggcgcctac
     1741 ccgcaccagc tctcgggcgg ccagcgccag cgcgccatga tcgccatggc gctggcctgc
     1801 gcgcccaagc tgctgctggc cgatgaaccc accaccgcgc tcgacgtcag cctgcgtggc
     1861 cagatgctgg agctgctcga cgacctgcgg cgcgaccacg gcatggccgt gctgctgatc
     1921 acgcacgacc tcaacctggt gcggcgattt gccgaccgcg tggcggtgat gcagcacggc
     1981 cggctggtgg agcagggcgc caccgaaagc atcttcaacg caccggagca tccctacacg
     2041 cgccagctga tggccagtcg ccccgaacgc gacgtggtcg aagccgcggc cgacgccggc
     2101 gccaccccgg cgctcagcgc gcagggtctg cgcgtggttt acccgacgcc gctgccgggc
     2161 atccggggct ggttccgcaa gggcgcgttc gttgcgctgc agggcgccga cttcgccatc
     2221 gccgccggtc gcacgctggg cgtgatcggt gaatccggct cgggcaaatc gaccctggcg
     2281 caggccgcac tgggcttgct gccacgcccg caggtggccg ggcaggtgca ggtaagcggc
     2341 cggtcctggc agggccgcgc gggcgccgac cggccgctgc ggcgcgcggt gcaggtggtg
     2401 tttcaggatc cgttttcatc gctgtcgcca cgcctgacgg tcgaagagat cgtcggcgaa
     2461 ggccttcgcg tgcacgcgcc cgtcatgccg ccggccgagc gcagccagcg ggttggccag
     2521 gcgctggccg acgtggggct gacgcaggcg gcctttccgg gcctgctggg gcgctacccg
     2581 cacgagttct ccggcgggca gcgccagcgc atcgccctgg cgcgcgccct gatcgtgcag
     2641 cccgagctgc tggtgctcga cgaaccgacc agcgcgctcg acgtcacgat ccagaagcag
     2701 gtactggggc tgctgcagca gctccagcgc gaacgtggtc tggcctacct gctgattacc
     2761 cacgacgtcg atgtggtgcg tgccatggcg cacgaggtgc tggtgctcaa ggacggcgcg
     2821 gtggtcgaag ccggtgctgc cgcgcaggtg ctgagccagc cgcagcaccc ctacacccag
     2881 cggctgctgg cggccgcctg acgggccgcg gcacacccgg gcgcggtgcc cggccgccgt
     2941 cgccgctggc gcgccgcagc ttgcgacggg cggtgtcaac ccaaaataga aatgtcccct
     3001 ttcagcccaa atagagatgt cccct
//
LOCUS       DAOOOG010000249         3024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_39507_length_3024_cov_20.966319, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000249 DAOOOG010000000
VERSION     DAOOOG010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39507_length_3024_cov_20.966319"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK242_12825"
     CDS             <1..480
                     /locus_tag="PK242_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HOZ94946.1"
                     /translation="LFSRWLIRSKYGRVLQAVRDAESRTMFCGYDPLPYKLSVWVISA
                     MMCGLAGALYVPQVGIINPSEMSVGNSIEMAVWTAVGGRASLVGPIIGAFAVNGGKSW
                     LTVAAPEYWLYVLGALFIVVTLFMPGGLIRLPGQIREWSARRRAQAGTSPALQGAQA"
     gene            477..1334
                     /gene="urtD"
                     /locus_tag="PK242_12830"
     CDS             477..1334
                     /gene="urtD"
                     /locus_tag="PK242_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HOZ94947.1"
                     /translation="MTPDLLEAGGERLERYRNTESGGRSASLARPVVPGEVDVTHGRI
                     LYLEDVHVSFDGFKAINGLQLDIAPGELRCIIGPNGAGKTTMMDIITGKTRPDRGTVF
                     FGSTIDLLRYREPEIAAMGIGRKFQKPTVFEQLSVFENLELALKTHKGVAASMRFRLG
                     GEQKDRIAEILHTIHLAGSVTRQAGLLSHGQKQWLEIGMLLAQEPKLLLLDEPVAGMT
                     DEETVRTAELFLSLKGKHSLMVVEHDMSFIETISEKVTVLCDGAVLAEGTLAQVQADE
                     RVIEVYLGR"
     gene            1388..2080
                     /gene="urtE"
                     /locus_tag="PK242_12835"
     CDS             1388..2080
                     /gene="urtE"
                     /locus_tag="PK242_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HOZ94948.1"
                     /translation="MLKVDNLHQYYGGSHILRNVSLRAEVGKVTVLLGRNGVGKTTLL
                     KSLMGLVPIKSGSLQWQGQDIARKTPYERARAGIGYVPQGREIFARLTVEENLRMGLA
                     YCPARTAIPAELYELFPVLKQMLHRRGGDLSGGQQQQLAIARALAPGPRLLILDEPTE
                     GIQPSIIKDIGRVIRMLAGRGDMAILLCEQYYDFAEELADHYLVMERGEVIAAGPGSE
                     MQQKEIRKLVAI"
     gene            2213..>3024
                     /locus_tag="PK242_12840"
     CDS             2213..>3024
                     /locus_tag="PK242_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HOZ94949.1"
                     /translation="MGWHAQLALDYRACHGRTVLHFTHDGPLRVLKSLYPEGPAICHN
                     VLVHPPGGLVGGDELDITVQVGAGAHALVTTPGAARFYKSNGQPARQRTRLRLATGAR
                     LEWLPLEAIAYNACDAHNHLEFELAEGAQMLAWDITALGLPLAGQAFALGRLTQHLEW
                     PGVYLERGLIDAQDGLLLDGDLGLAGQRCLASLVLASGTPLPRSTREQLLEDMHALLQ
                     CGHGSVLAGVTAPNDHMLVLRALAPVVEPAMALCRSAWSLWRGQLWGLRGDMP"
BASE COUNT          523 a          997 c         1038 g          466 t
ORIGIN      
        1 ctgttctcgc gctggctcat ccgcagcaaa tatggccgcg tgctgcaggc cgtgcgcgat
       61 gccgagtcgc gcaccatgtt ctgcggctac gacccgctgc cgtacaagct gtcggtctgg
      121 gtcatctcgg ccatgatgtg cggcttggcc ggcgcgctct acgtgccgca ggtgggcatc
      181 atcaacccca gcgagatgag cgtgggcaac tcgatcgaga tggccgtgtg gacggccgtg
      241 ggcgggcgcg ccagcctggt ggggcccatc atcggcgcgt ttgcggtcaa tgggggcaag
      301 agctggctca cggtggcggc gcccgaatac tggctgtatg tgctcggcgc gctgttcatc
      361 gtggtgacgc tgttcatgcc gggcggcctg atccgcctgc ccggccagat ccgcgaatgg
      421 agcgctaggc gtcgcgccca ggccggcaca tcgccagccc tgcaaggagc ccaggcatga
      481 cccccgatct gctggaagcc ggcggcgagc gcctggagcg ctaccgcaac accgaatcgg
      541 gcgggcgcag cgccagcctg gcgcggcccg tggtgccggg cgaggtggat gtgacgcacg
      601 gccgcatcct gtatctggag gacgtgcacg tcagcttcga cggcttcaag gccatcaacg
      661 gccttcagct tgacatcgcg cccggcgagc tgcgttgcat catcggcccc aacggcgccg
      721 gcaagaccac gatgatggac atcatcaccg gcaagacccg gcccgacaga ggcacggtgt
      781 tcttcggctc caccatcgac ctgctgcgct accgcgagcc cgaaatcgcc gccatgggca
      841 ttggccgcaa attccagaag cccaccgtgt tcgagcagct cagcgtgttc gaaaacctgg
      901 agctggcact gaaaacccac aagggcgtgg cagcgtccat gcgtttcagg ctgggcggcg
      961 agcagaaaga ccgtatcgcc gaaatcctgc acaccatcca cctggccggc agcgtgacgc
     1021 gccaggccgg cctgctgagc cacggccaga agcagtggct ggagatcggc atgctgctgg
     1081 cgcaggagcc caagctcttg ctgcttgacg agccggtggc gggcatgacc gacgaggaaa
     1141 ccgtgcgcac ggccgagctg tttctgtcgc tcaagggcaa gcattcgctg atggtggtgg
     1201 agcacgacat gagcttcatc gaaaccatca gcgagaaggt cacggtgctg tgcgacggcg
     1261 cggtactggc cgagggcacg ctggcccaag tccaggccga cgagcgtgtg atcgaggttt
     1321 atctgggccg ataaactatc aatgtaatag ctgctcgcgc ttgatacaca agggctggag
     1381 gccaaaaatg cttaaagtcg acaacctgca ccagtactac ggcggctcgc acattctgcg
     1441 caacgtcagc ctcagggccg aggtcggcaa ggtcacggtg ctgctgggcc gcaacggcgt
     1501 gggcaagacc acgctgctca agagcctgat ggggctggtg ccgatcaaga gcggcagcct
     1561 tcagtggcag ggccaggaca tcgcgcgcaa gacgccctat gaacgcgccc gcgccggcat
     1621 cggctacgtg ccgcaggggc gcgagatctt tgcgcgcctg acggtcgagg agaatctgcg
     1681 catgggactg gcttactgcc ccgcgcgcac cgccatcccg gccgagttgt acgagctgtt
     1741 cccggtgctc aaacagatgc tgcaccgccg cggcggcgat ctttcgggcg ggcagcagca
     1801 gcagctggca attgcccgcg cgctggcgcc gggccccagg ctgctgatcc tggacgagcc
     1861 gaccgagggc atacagccca gcatcatcaa ggacatcggc cgcgtgattc gcatgctggc
     1921 cgggcgcggc gacatggcca tcctgctgtg cgagcaatac tatgactttg ccgaagaact
     1981 ggccgaccac tatctggtga tggagcgcgg cgaagtgatt gccgccgggc cgggcagcga
     2041 aatgcagcaa aaggaaatcc gcaaactggt ggccatctga aatcgcccaa gcaaccctgc
     2101 atgcgcatgc tgaccacccc gatgccaccg cctggccttg cgcaggcgcg ccgcagtctg
     2161 cgcgggggcg acggcgcggc gcaacggcgg tccacgcacg gcttctgaat ccatgggctg
     2221 gcatgcgcaa ctggcgctcg actatcgcgc atgccacggc agaaccgtgc tgcacttcac
     2281 gcacgacggc ccgctgcgcg tgctcaagag cctgtacccc gaaggcccag ccatctgcca
     2341 caacgtgctg gtgcacccgc ccggcgggct ggtcggcggc gacgagctcg acatcacggt
     2401 gcaggtcggg gccggcgccc atgcgctggt gaccacgccg ggcgcagcgc gcttttacaa
     2461 aagcaacggc caaccggcgc gccagcgcac gcggctgcgc ctggccaccg gggcgcggct
     2521 tgaatggctg ccgctggagg ccattgccta caacgcctgc gacgcgcaca accaccttga
     2581 atttgagctg gccgaaggcg cgcagatgct ggcctgggac atcaccgcgc tgggcctgcc
     2641 gctggctggt caggccttcg cgctgggccg cttgacgcag cacctggagt ggccgggggt
     2701 gtatctggag cgcggcctga tcgacgcgca ggatggcctg ctgctcgatg gcgacctggg
     2761 cctggccggc cagcgctgcc tggccagcct ggtgctggcc agcggcaccc cactgccgcg
     2821 ttcgacgcgc gaacaactgc tggaggacat gcacgcgctg ctgcaatgcg ggcacggcag
     2881 cgtgctggcc ggcgtgaccg cgcccaacga tcacatgctg gtgctgcgcg cgctggcgcc
     2941 agtggtggag ccggccatgg ccctgtgcag gagcgcctgg tccctgtggc gcggccagct
     3001 gtggggcctg cgcggcgaca tgcc
//
LOCUS       DAOOOG010000250         3010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_39768_length_3010_cov_26.274788, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000250 DAOOOG010000000
VERSION     DAOOOG010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39768_length_3010_cov_26.274788"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK242_12845"
     CDS             complement(<1..211)
                     /locus_tag="PK242_12845"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829694.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOZ94950.1"
                     /translation="MTDRLIIFDTTLRDGEQSPGASMTKDEKLRIARQLERLRVDVIE
                     AGFAASSNGDFECVKAIAEVIKDSTV"
     gene            complement(443..1321)
                     /gene="pssA"
                     /locus_tag="PK242_12850"
     CDS             complement(443..1321)
                     /gene="pssA"
                     /locus_tag="PK242_12850"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOZ94951.1"
                     /translation="MPDHEFSTPRPPDDLPPPRRRKGIYVLPNMITLAALFGGFYAIV
                     MAMHGQFAAAATGIFAAMVLDSLDGRVARMTHTQSAFGEQMDSLSDMVSFGAAPALVA
                     YMWALQPLGKAGWFAAFVYCACAALRLARFNVNTGVVDKRYFQGLPSPAAAALVAGFI
                     WLTSTMIEAQREVPLFRALISALGGARVARGDFSWLLFAITLFAGLTMVTNTPYYSFK
                     DLRGRRSVPFVMLVLAALLIAVISIEPATMLFLVFVGYALSGYVVYVWRKAKGEHASI
                     VSTSTDEPDERGLHHD"
     gene            complement(1549..2565)
                     /gene="ilvC"
                     /locus_tag="PK242_12855"
     CDS             complement(1549..2565)
                     /gene="ilvC"
                     /locus_tag="PK242_12855"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOZ94952.1"
                     /translation="MKVFYDKDCDLSLIKGKTVAIIGYGSQGHAHAQNLNDSGVKVVV
                     GLRKGGASWAKVEKAGLKVMEVNDAVKAADVVMILLPDEQIAEVYRNNVEPNIKSGAS
                     LAFAHGFNVHYNQVVPRADLDVWMVAPKAPGHTVRNTYTQGGGVPHLVAVHQDKSGKA
                     RDLALSYAMANGGGKAGIIETNFKEETETDLFGEQAVLCGGTVELIKMGYETLVEAGY
                     APEMAYFECLHELKLIVDLIYEGGIANMNYSISNNAEYGEYVTGPKVVNESSRQAMRE
                     ALRNIQNGDYAKQFILEGRTAYPSMTARRRNMSEHSIEVVGAKLRAMMPWIAKNKLVD
                     QTRN"
     gene            complement(2640..>3010)
                     /gene="ilvN"
                     /locus_tag="PK242_12860"
     CDS             complement(2640..>3010)
                     /gene="ilvN"
                     /locus_tag="PK242_12860"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOZ94953.1"
                     /translation="SLSRMTIQTRGSDEVIEQITKHLNRLIEVVKVVDLTEGSYIERE
                     HMMVKVRAVGKEREEIKRMADIFRGRIIDVTDKSYTIELTGDQGKHDAFLNAIDKSAI
                     LETVRTGASGIGRGERLLKV"
BASE COUNT          486 a          956 c         1002 g          566 t
ORIGIN      
        1 acacggtgga gtccttgatc acctcggcga tcgccttgac gcactcgaag tcgccgttgc
       61 tgctggcggc aaagccggcc tcgatcacgt ccacccgcag gcgctcgagc tggcgtgcga
      121 tgcgcagctt ttcgtccttg gtcatgctgg cgccgggcga ttgttcgccg tcgcgcaagg
      181 tggtgtcgaa gatgatgagt cggtcggtca tgggctgttc tccggaaaaa tgaagcttgg
      241 gattcccgcc agcgaaaaac gtcttgagca aacaaaaagg cccgctgcgg gtgcaaacgg
      301 gcctttacag gaaaaagttg cgcgcgctac cgtccccgcc cgttcggcgt tgccagtcgt
      361 agcgccagcg acttgttcat ggaacggtaa ctgtagcaca tggcttggct ggcgtgtgca
      421 tcggtgattt caccgagcgc gctcagtcat ggtgcagccc gcgttcgtca ggctcgtcgg
      481 tggaggtgct gacgatgctg gcgtgctcgc ccttggcctt gcgccacaca tacaccacgt
      541 agcccgacag cgcatagccg acgaacacca gaaacagcat ggtggccggc tcgatgctga
      601 tgacggcgat cagcagcgcc gccagcacca gcatgacgaa gggcacgctg cgccgcccgc
      661 gcaggtcctt gaagctgtag tacggcgtgt tggtgaccat ggtgagcccg gcgaacagcg
      721 tgatggcgaa cagcagccag gaaaagtcgc cacgcgccac gcgcgcgccg ccgagcgccg
      781 agatcagggc acgaaacagc ggcacctcgc gctgcgcctc gatcatggtg ctggtcagcc
      841 atatgaagcc cgccaccagc gccgccgccg ccggcgaggg caggccctgg aagtagcgct
      901 tgtcgaccac gccggtgttg acgttgaagc gcgccagccg cagcgccgcg caggcgcagt
      961 agacgaaggc cgcgaaccag cccgccttgc ccagcggctg cagggcccac atgtaggcca
     1021 ccagcgccgg cgcggcaccg aagctgacca tgtccgacag cgaatccatc tgctcgccga
     1081 aggcgctttg ggtgtgcgtc atgcgcgcca cgcggccgtc caggctgtcg agcaccatgg
     1141 cggcgaagat gccggtggcg gcggcggcaa actgcccgtg catggccatg acgatggcgt
     1201 agaagccacc gaacagcgcc gccagcgtga tcatgttggg cagcacatag atgcccttgc
     1261 ggcgccgggg tggcggcagg tcgtccggtg gccgtggggt cgaaaactcg tggtcaggca
     1321 tcagattgca gctcatgccg cccgcagaca gcgcgagcag ctatgaaatt catagtatgg
     1381 cggcagcccc gacggggccg ccgctgccgt gcagtgtaac gactcgggca caaaaaaggc
     1441 cacgcggggc gtggcctcgg gggacgggtg gcacgcgcga gccgcccaag tacggccgtg
     1501 gctcgcgccc gcaggcagcg cgccagcggc ccaccgccgt ggtcaggatc agttgcgcgt
     1561 ctgatcgacc agcttgttct tggcgatcca cggcatcatg gcgcgcagct tggcgcccac
     1621 cacctcgatg ctgtgctcgg acatgttgcg gcggcgcgcg gtcatgctgg gataggcggt
     1681 gcggccttcc aggatgaact gcttggcgta gtcgccgttc tggatgttgc gcagcgcctc
     1741 gcgcatggcc tggcgcgagc tttcgttgac gaccttgggg ccggtcacgt actcgccata
     1801 ttcagcgttg ttgctgatcg agtagttcat gttggcgatg ccgccttcat agatcagatc
     1861 gacgatcagc ttgagctcgt gcaggcactc gaagtaggcc atttcgggcg cgtagccggc
     1921 ctcgaccagg gtctcgtagc ccatcttgat cagctcgacg gtgccgccgc acagcacggc
     1981 ctgttcgccg aacaggtcgg tctcggtctc ttccttgaag ttggtctcga tgatgccggc
     2041 cttgccgccg ccgttggcca tggcgtagga cagcgccaga tcgcgcgcct tgccgctctt
     2101 gtcctggtgc accgccacca ggtgcggcac gccgccgccc tgggtgtagg tgttgcgcac
     2161 ggtgtggccg ggggccttgg gcgccaccat ccacacgtcc aggtcggcgc gcggcaccac
     2221 ctggttgtag tgcacgttga agccgtgggc aaaggccagc gaggcgccgg acttgatgtt
     2281 gggctcgacg ttgttcctgt acacctcggc gatctgctcg tcgggcagca ggatcatcac
     2341 cacgtcggcg gccttgacgg cgtcgttgac ctccatcacc ttcaggccgg ccttttcgac
     2401 cttggcccag ctggcgccgc ccttgcgcag gccgaccacc accttgacac cgctgtcgtt
     2461 caggttctgg gcgtgggcgt ggccctgcga gccgtagccg atgatggcca cggtcttgcc
     2521 cttgatcagg ctcaggtcac agtctttgtc gtagaaaact ttcacgggtt tctccttctg
     2581 atgctttgct tgctatggaa taaagagctg cttgcgcagg atgggcgggc gctgtggcct
     2641 caaaccttca gcagtcgttc gccacgcccg atgccgctgg cgccggtgcg cacggtctcc
     2701 aggatggcgg atttgtcgat cgcgttcagg aaggcgtcgt gcttgccctg gtcgccggtg
     2761 agctcgatgg tgtagctctt gtcggtgacg tcgatgatgc ggccgcggaa gatgtcggcc
     2821 atgcgcttga tttcctcgcg ctccttgccg acggcgcgca ccttgaccat catgtgctcg
     2881 cgctcgatgt agctgccctc ggtcagatcg accaccttga ccacctcgat caagcggttc
     2941 aggtgcttgg tgatctgctc gatcacctcg tcggagccgc gcgtctggat ggtcatgcgc
     3001 gacaggctgg
//
LOCUS       DAOOOG010000251         2962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_40683_length_2962_cov_29.899897, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000251 DAOOOG010000000
VERSION     DAOOOG010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40683_length_2962_cov_29.899897"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PK242_12865"
     CDS             <1..213
                     /locus_tag="PK242_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94954.1"
                     /translation="DAPLDDANTERYAKLVTRMSGQGTQFLFISHNKIAMEMAEQLIG
                     VTMQEQGVSRIVAVDMESAVGMAEAS"
     gene            299..1366
                     /locus_tag="PK242_12870"
     CDS             299..1366
                     /locus_tag="PK242_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HOZ94955.1"
                     /translation="MSSLQVGLAVAGGLILGGVIAYNTWNARRHQPRQAQPEAPAPAE
                     RVEPGLQEAMAAPERQEPSFDAEAALPSLGPLPLPDKRPRLDALIDVIAPIALDGRVV
                     SGDALLAALPATRRVGSKPFAVEGQHDTRGEWEFPQAGQRYHAVQAGVQLANRTGPLN
                     DIEFSEFVMCAQRYADAVGGAPEFPDMLHEVARARELDQFASAHDAQLGFTLRARGAA
                     WSPGFVQQQAARQGFVAGAIPGRMVLPAAEPGLPPLLDLSFDAQAALSEDVAHAAITA
                     LVLNLDVPQVHRAERPFDRLRDIAFALAEAMDGVITDDAGQPLRPELIDGIGADLDRL
                     YDTLEQHDLAAGSPQARRLFS"
     gene            1416..>2962
                     /gene="ligA"
                     /locus_tag="PK242_12875"
     CDS             1416..>2962
                     /gene="ligA"
                     /locus_tag="PK242_12875"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOZ94956.1"
                     /translation="MNNEANVSRRAAELRARLNRAAHQYYTLDAPELPDAEYDRLFRE
                     LQAIEAEHPALRTPDSPTQRVGGAVLDTLAPVRHAVPMLSIRTETDTSPQGAATFDQR
                     VRDYLASEQRRTLLKLDDERHRSQPLGPAAQAVLAGAPIDYVAEPKFDGLAMSLRYER
                     GVLVQAATRGDGETGEDVTHNIRTIGQIPLRLVAPTLAAARAALPPEGAGFALGRPGG
                     ETAAGVPPLLEVRGEVYMRRDDFERLNEVMRQRMAAGDKAAKTFVNPRNTAAGAVRQL
                     DAALAAERRLSFFAYGLGEVTAPADGGPRFDTHWAVLQALKSWGFPVSALARQARGAS
                     ELIAFHADVAAQRDALPFDIDGVVYKVDSLELQRELGFVTREPRWAVAHKYPAQEELT
                     TVQAIDVQVGRTGKLTPVAKLAPVFVGGVTVTNATLHNELEARRKDVRVGDTVVVRRA
                     GDVIPEVVSVLPDQRPPGTAEFTMPASCPVCGSAVVREEGEADHRCTGGLFCPAQRKQ
                     ALLHFASR"
BASE COUNT          441 a         1087 c         1035 g          399 t
ORIGIN      
        1 gacgcgccgc tggacgacgc caacaccgag cgctacgcca agctggtcac gcgcatgagc
       61 gggcagggca cgcagttcct gttcatcagc cacaacaaga tcgccatgga aatggccgag
      121 cagctgatcg gcgtcaccat gcaggagcag ggcgtctcgc gcatcgtggc ggtggacatg
      181 gaaagcgccg tcgggatggc cgaagcatca tgagcgcgcc tgtgcgtcgg ccggccccgc
      241 ggcccctgat ccggcgggtc ctgcccggcg tgcgcggcgc ccgaggagct gtgtcgacat
      301 gagttctctg caagtgggcc tggcggtggc cggtggcctg atcctgggcg gcgtaatcgc
      361 ctacaacacc tggaacgcgc gtcgccacca gccgcgccag gcgcagcccg aagcgccggc
      421 gcccgccgag cgcgtggagc ccgggctgca ggaggccatg gcggcgcccg agcgccagga
      481 gcccagcttc gacgccgaag ccgcactgcc ttcgctcggc cccttgccgc tgcccgacaa
      541 gcgtccgcgg ctggatgcgc tgatcgacgt catcgccccc atcgcgctgg acggccgggt
      601 ggtgtcgggc gacgccttgc tggcggcgct gccggccacg cgccgtgtgg gcagcaagcc
      661 gttcgcggtc gaaggccagc acgacacccg tggcgagtgg gagtttcccc aggccggcca
      721 gcgctaccac gcggtgcagg cgggggtgca gctggccaac cgcaccggcc cgctcaacga
      781 catcgagttt tccgaattcg tcatgtgtgc gcagcgctac gccgatgccg tgggtggtgc
      841 gccggaattt cccgacatgc tgcacgaagt ggcccgcgcg cgcgaactcg accagttcgc
      901 cagcgcccat gacgcgcagc tgggctttac cctgcgcgcg cgcggcgccg cctggagccc
      961 cggtttcgtg cagcagcagg cggcgcgcca gggcttcgtg gccggcgcca tccccggccg
     1021 catggtgctg cccgccgccg agccggggct gccgccgctg ctggacctga gtttcgacgc
     1081 ccaggccgcg ctgtccgagg acgtagcgca cgccgccatc acggcgctgg tgctcaacct
     1141 ggacgtgccg caggtgcacc gggccgagcg cccgttcgac cgcctgcgcg acatcgcctt
     1201 cgcgctggcc gaggccatgg acggggtcat caccgacgac gccggccagc cgctgcgacc
     1261 cgagctgatc gatggcatcg gcgccgacct ggaccgcctg tacgacacgc tggagcagca
     1321 cgacctggcg gccggctcgc cgcaggcgcg ccggctgttc agctgatttc agattgtttt
     1381 ggcctccagc gcccgcccag agtgcgcgag cagctatgaa taacgaagca aacgtaagcc
     1441 gacgagcggc cgagctgcgg gcccggctga accgcgccgc ccaccagtac tacacgctcg
     1501 acgcgcccga actgcccgac gccgagtacg accgcctgtt tcgcgagttg caggccatcg
     1561 aggccgaaca ccccgcgctg cgcacgcccg actcgcccac ccagcgcgtg ggcggtgccg
     1621 tgctcgacac gctggcaccg gtgcgccacg ccgtgcccat gctcagcatc cgcaccgaaa
     1681 ccgacacctc gccccagggc gcggccacct tcgatcagcg cgtgcgcgac tatctggcca
     1741 gcgagcagcg gcgcaccctg ctcaagctcg acgacgagcg ccaccgcagc cagccgctgg
     1801 gcccggccgc gcaggccgtg ctggctggcg cgcccatcga ctacgtggcc gaacccaagt
     1861 tcgacggcct ggccatgagc ctgcgctacg agcgcggcgt gctggtgcag gccgccacgc
     1921 gcggcgacgg cgaaaccggc gaagacgtca cgcacaacat ccgcaccatc ggccagatcc
     1981 cgctgcgtct ggtcgccccc acgcttgccg ctgcgcgtgc tgcgctgccc cccgaggggg
     2041 ctggtttcgc cttggggcgg cccggcggcg aaaccgctgc cggcgtgccg ccgctgctcg
     2101 aagtgcgcgg cgaggtctat atgcgccgcg acgatttcga gcgcctgaac gaggtcatgc
     2161 gccagcgcat ggccgccggc gacaaggccg ccaagacctt cgtcaacccg cgcaacaccg
     2221 ccgccggcgc ggtgcgccag ctcgatgccg cgctggcggc cgaacggcgg ctgagctttt
     2281 tcgcctacgg cctgggcgag gtgacggcgc ccgccgacgg cggccctcgg ttcgacaccc
     2341 attgggccgt gctgcaggcc ctgaaatcat ggggcttccc ggtctccgcg cttgccagac
     2401 aagcgcgagg cgcttcagag ttgatagcat ttcacgccga tgtggcagcc cagcgcgacg
     2461 cgctgccctt cgacatcgac ggcgtggtct acaaagtgga ttcactcgaa ctgcagcgcg
     2521 agctgggctt cgtcacgcgc gagccgcgct gggcggtggc gcacaaatac ccggcgcagg
     2581 aagagctgac caccgtgcag gcgatcgacg tgcaggtcgg gcgcaccggc aagctcacgc
     2641 cggtggccaa gctggcgccg gtgttcgtgg gcggcgtcac cgtcaccaac gccaccctgc
     2701 acaacgagct ggaagcgcgc cgcaaggacg tgcgcgtggg cgacaccgtg gtggtgcgcc
     2761 gcgccggcga cgtgattccc gaggtggtgt cggtgctgcc ggaccagcgc ccgccgggca
     2821 cggctgaatt caccatgccc gccagctgcc cggtgtgtgg cagcgcggtg gtgcgcgagg
     2881 agggcgaggc cgaccaccgt tgcaccggcg gcctgttctg cccggcgcag cgcaagcagg
     2941 cgctgctgca ctttgccagc cg
//
LOCUS       DAOOOG010000252         2950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_40914_length_2950_cov_34.509154, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000252 DAOOOG010000000
VERSION     DAOOOG010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40914_length_2950_cov_34.509154"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PK242_12880"
     CDS             <1..476
                     /locus_tag="PK242_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOZ94957.1"
                     /translation="CQDGWVLVAGNGDSIFKRLMLAIGRADLAAAPDLADNAGRVARV
                     DEIDAAIGAWTEPRTVQAVLDALAAARVPAGKVYTARDIAEDPHYRAREMIVAQRTRD
                     GDVVEVPGVVPKLSVTPGTLRRSAPHLGDDTDAVLRELGLDAAQIAALRQRGIVQ"
     gene            complement(559..2430)
                     /locus_tag="PK242_12885"
     CDS             complement(559..2430)
                     /locus_tag="PK242_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HOZ94958.1"
                     /translation="MSASKSSLASLTLGAIGVVYGDIGTSVLYTLKEVFGSGHVPFTT
                     DNVYGVLSIVFWTLTIIVSLKYVTLVLRADNHGEGGLVAMLALASQSVKHKAPALRKW
                     MLLIGIFGTCLFYGDGVITPAITVLSAVEGLDVVSPAFRKAVIPLTLLILLALFWVQK
                     RGTAGIGRFFGPIMVLWFLAIAALGVHHIAGHPEILAALSPHHAIGFIVRQPGTSFII
                     LGAVVLCVTGGEALYADMGHFGKRPIRVAWFSIVMPCLTLNYFGQGALLLSEPQAVSN
                     PFFHMAPDWLTLPLVGLATMAAVIASQALISGAFSVTKQVIQLGYLPRLQIQHTSTRD
                     TGQIYMPFVNWSLFVMIVLAVVLFRSSSNLAAAYGIAVTLDMTITTVLTFFVIRYGWG
                     YPLALCIAATGFFFVIDLAFFSSNLLKLAEGGWFPILIGGAVFTLMVTWKDGRELLSR
                     SLRDGSIDLKSFLESVLLSPPTRVPGTAVFLSAEPGIVPNALMHNLKHNKVLHAQNLF
                     VTRRAHEVPWIGLDKRLEVQALGSDCWQVVVHYGFKNEPDLPKALELLAGRGIDLNPM
                     TTSYFLSRDIVTPTLGSGMAFWREKLFAAMHHGASAAAEYLNLPSNAVVELGSKVEI"
     gene            complement(2491..>2950)
                     /locus_tag="PK242_12890"
     CDS             complement(2491..>2950)
                     /locus_tag="PK242_12890"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOZ94959.1"
                     /translation="VVKADQGTRGMGIMTVRDVKDLAALRQPARKADKGAASDIPRGE
                     VIIQEGVLTNERVHDAVAEPVVYLIDRYVVGGFYRIHAERGVDENLNAPGARFVPLPF
                     AGNAHLPRPGGKGEARPPNRFYMYGVIARLAMVAASYELEATDPELQALA"
BASE COUNT          489 a          979 c         1046 g          436 t
ORIGIN      
        1 cctgccagga cggctgggtg ctggtggccg gcaatggcga cagcatcttc aagcgcctga
       61 tgctggccat cggccgcgcc gacctggccg ctgcgcccga cctggccgac aacgccggcc
      121 gggtggcgcg cgtggacgag atcgacgccg ccatcggcgc ctggaccgag ccgcgcaccg
      181 tgcaggcggt gctggacgcg ctggcggcgg cgcgtgtgcc ggccggcaag gtgtacaccg
      241 cgcgcgacat cgccgaagac ccgcactacc gcgcgcgcga gatgatcgtg gcgcagcgca
      301 cgcgcgacgg cgacgtggtc gaggtgcccg gcgtggtgcc caagctgtcg gtcacgcccg
      361 gcaccctgcg ccgcagcgcc ccccacctgg gcgacgacac cgacgccgtg ctgcgcgagc
      421 tgggcctgga cgcggcgcag atcgcggcgc tgcgccagcg cggcatcgtg cagtgaaccg
      481 ggacaccgcg gctcccggaa tactatataa ataatagctt ttggcgcttg tctggcaagc
      541 gctggagctc gttttggctc agatttcgac cttgctgccc aactccacca cggcgttgct
      601 gggcaggttc aggtattcgg ccgccgccga ggcgccgtgg tgcatggcgg cgaacagttt
      661 ttcgcgccag aacgccatgc ccgagccgag cgtcggcgtg acgatgtcgc gcgacaggaa
      721 gtagctggtg gtcatggggt tgaggtcgat gccgcggccg gccagcagct ccagcgcctt
      781 gggcaggtcg ggctcgttct tgaagccgta gtgcaccacc acctgccagc agtcgctgcc
      841 cagcgcctgc acttccagcc gcttgtccag gccgatccag ggcacctcgt gggcgcgccg
      901 ggtgacgaac aggttctgcg cgtgcagcac cttgttgtgc ttgaggttgt gcatcagcgc
      961 gttgggcacg atgccaggct cggcgctcag gaacaccgcg gtgccgggca cgcgcgtggg
     1021 cggactgagg agtaccgatt cgaggaagga cttcaggtcg atcgagccgt cgcgcaggct
     1081 gcggctgagc agttcgcggc cgtccttcca ggtcaccatc agggtgaaca cggcgccgcc
     1141 aatcaggatc gggaaccagc cgccttcggc cagcttgagc aggttggagc tgaagaacgc
     1201 caggtcgatg acgaagaaga agccggtcgc cgcgatgcac agcgccagcg ggtagcccca
     1261 gccgtagcgg atgacgaaga aggtcagcac cgtggtgatg gtcatgtcca gcgtcaccgc
     1321 gatgccgtag gctgccgcca ggttggacga ggagcggaac agcaccaccg ccagcacgat
     1381 catcacgaac aggctccagt tgacgaaggg catgtagatc tggccggtgt cgcgcgtgct
     1441 ggtgtgctgg atctgcaggc gcggcaggta gcccagctgg atcacctgct tggtgacgct
     1501 gaaggcgccc gaaatcagcg cctgcgaggc gatcaccgcc gccatggtgg ccagcccgac
     1561 cagcggcagg gtcagccagt cgggcgccat gtggaagaac gggttgctga cggcctgcgg
     1621 ctccgacagc agcagcgcgc cctggccgaa gtagttcagc gtcaggcagg gcatgacgat
     1681 ggagaaccag gccacacgga tcggccgctt gccgaaatgc cccatgtcgg cgtacagcgc
     1741 ctcgccgccg gtgacgcaca gcaccaccgc gcccaggatg atgaagctgg tgcccggctg
     1801 tcgcacgatg aagccaatgg cgtggtgcgg gctgagcgcg gccaggatct ccgggtggcc
     1861 ggcgatgtga tgcacgccca gcgccgcgat ggccaggaac cacagcacca tgatcggccc
     1921 gaaaaagcgc ccgatgccgg ccgtgccgcg cttttgcacc cagaacagcg ccagcaggat
     1981 cagcagcgtg agcgggatca cggccttgcg gaacgcgggc gagaccacgt ccagcccctc
     2041 caccgccgac agcaccgtga tggccggggt gatgacgccg tcgccgtaga acaggcaggt
     2101 gccgaagatg ccgatcagca gcatccactt gcgcagcgcc ggcgccttgt gcttgaccga
     2161 ctgcgaggcc agcgccagca tggccaccag gccgccttcg ccgtggttgt cggcgcgcag
     2221 caccagggtg acgtatttca gcgagacgat gatcgtcagc gtccagaaca cgatcgacag
     2281 cacgccgtag acgttgtcgg tcgtgaacgg cacatggccg ctgccgaaca cctccttgag
     2341 cgtgtacagc acgctggtgc cgatgtcgcc atacaccacg ccgatggcgc ccagcgtcag
     2401 gctggcgagg gaagacttgg aagccgacat ggcgtaggga tgttcttgcg ggagcgcgat
     2461 ggtgcgcccg ccgcggtggt gcggtccgcc tcaggccagg gcctgcagct ccgggtcggt
     2521 ggcttccagc tcgtagctgg ccgccaccat ggccaggcgg gcgatcacgc cgtacatgta
     2581 gaagcggttg ggcgggcgcg cctcgccctt gccgcccggc cgcggcaggt gggcgttgcc
     2641 ggcaaagggc agcggcacga agcgcgcgcc gggggcgttg aggttctcgt ccacgccgcg
     2701 ctcggcgtgg atgcggtaga agccccccac cacgtagcga tcgatcaggt acaccaccgg
     2761 ctcggccacc gcgtcgtgga cgcgctcgtt ggtcagcacg ccctcctgga tgatgacttc
     2821 gccgcgcggg atgtcggacg ccgcgccctt gtcggccttg cgcgccggct gccgcagcgc
     2881 agcgaggtcc ttgacgtcgc gcaccgtcat gatgcccatg ccgcgcgtgc cctggtcggc
     2941 cttgacgacc
//
LOCUS       DAOOOG010000253         2937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_41162_length_2937_cov_25.917765, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000253 DAOOOG010000000
VERSION     DAOOOG010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41162_length_2937_cov_25.917765"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /gene="frr"
                     /locus_tag="PK242_12895"
     CDS             <1..491
                     /gene="frr"
                     /locus_tag="PK242_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOZ94960.1"
                     /translation="LTKIRTGRANPALLDTIHVEYYGNHVPLSQVANVSLLDARTISV
                     QPWEKGMGAKIEKAIRESDLGLNPASMGDLIRVPMPPMSEERRREMTKIVRHEGEQAK
                     IAIRNLRRDANDAIKKLVKDKEATEDEQKRSEADIQKVTDRHVADIDALVAAKEKDIM
                     EV"
     gene            627..1358
                     /gene="uppS"
                     /locus_tag="PK242_12900"
     CDS             627..1358
                     /gene="uppS"
                     /locus_tag="PK242_12900"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOZ94961.1"
                     /translation="MANASTSHELPRHIAIVMDGNGRWARRRLLPRIAGHRQGVDALR
                     RCIRACAERGVQVLTVFAFSSENWSRPQDEVSGLMELLGKALTREVPQLQRAGIRLHF
                     VGERQALSEGMRHALAQAEADTTGNAGMVLNVCFNYGGRWDIVQAAARLAERGAPITE
                     ATLAGAMALAHVPDPDLLIRTGGEQRISNFLLWQCAYSELYFTDCLWPDFDAAALDAA
                     LAAYAGRERRFGRTSEQLAQAGAAG"
     gene            1372..2238
                     /locus_tag="PK242_12905"
     CDS             1372..2238
                     /locus_tag="PK242_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOZ94962.1"
                     /translation="MLGQRVITALVLLAVLLPALFAPSATPFIALSLLFIAAAAWEWA
                     RLNGVAGAGALATGALCAALCLGLWALGAVERPLPGLWLAAALAWVLGGAWVLARGVA
                     GWARVPAALRWAMGLALLSVAWLAMAQARRIGVNFLLSAMVLVWVADVFAYFAGRALG
                     GRLTGGNKLAPTISPGKSWEGVWGGMLGVLALAVLWLWLDRTQPLGAASIYTRLHQAG
                     PALLVAGVLFLCAMSVVGDLFESLVKRSAGMKDSSQLLPGHGGVLDRIDALLPTLPLA
                     MLLASWSAGGGA"
     gene            2235..>2937
                     /locus_tag="PK242_12910"
     CDS             2235..>2937
                     /locus_tag="PK242_12910"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOZ94963.1"
                     /translation="MRRQRLAVLGSTGSIGTSTLDVVARHPERFEVVALTAATQVDAM
                     LAQCERFRPRVAVMASAPHAAALAQKIKQKGIPTQVEQAPEAIENIACWGEVDTVMAA
                     IVGAAGLAPCLAAARAGKRLLLANKEALVVGGEVFLRAVQAGGATLLPIDSEHSAIFQ
                     SLPEDPATWAARIDKIILTASGGPFRTRAPESLAAVTPEQACAHPNWVMGRKISVDSA
                     TMMNKALEVIEARYLF"
BASE COUNT          422 a          980 c         1091 g          444 t
ORIGIN      
        1 acctgaccaa gatccgcacc ggccgcgcca acccggcgct gctggacacc atccatgtcg
       61 agtactacgg caaccacgtg ccactgtcgc aggtggccaa cgtgtcgctg ctggatgcgc
      121 gcaccatcag cgtgcagccg tgggaaaagg gcatgggtgc caagatcgag aaggccatcc
      181 gcgaaagcga cctgggcctg aacccggcca gcatgggcga cctgatccgc gtgccgatgc
      241 cgcccatgag cgaggaacgc cggcgcgaga tgaccaagat cgtgcggcac gagggcgagc
      301 aggccaagat cgccatccgc aacctgcgcc gcgacgccaa cgacgccatc aagaagctgg
      361 tcaaggacaa ggaagccacc gaggacgagc aaaaacgctc cgaggccgat atccagaaag
      421 tcactgaccg gcacgtggcc gacatcgacg ccctggtggc ggccaaggaa aaggacatca
      481 tggaggtctg acctccggcg ctcgccggca gcgccatccg ctcaggggcg ctgcgggcga
      541 ccgggaggct cggacagggc gtactggcct gctccgcggc ccatgggcgg gccggcctcg
      601 cagccccctg gaccggttcc gcgcacatgg caaacgcttc cacttcccac gagctgccgc
      661 gacacatcgc catcgtgatg gatggcaatg gccgctgggc gcggcggcgc ctgttgccgc
      721 gcatcgccgg tcatcggcag ggggtcgacg ccctgcgccg ctgcatccgt gcctgcgccg
      781 agcgcggcgt acaggtgctg acggtgtttg ccttctcctc cgaaaactgg agccggccgc
      841 aggacgaggt cagtggcctg atggaactgc tgggcaaggc gctgacgcgc gaggtgccgc
      901 agttgcagcg cgccggcatc cgcctgcatt tcgtgggcga gcggcaggcg ttgtccgagg
      961 gcatgcggca cgccctggca caggccgagg ccgacaccac cggcaacgcc ggcatggtgc
     1021 tgaatgtgtg cttcaactat ggcggccgct gggacatcgt gcaggccgcc gcccgactgg
     1081 ccgagcgcgg tgcgccgatc accgaggcca cgctggccgg cgccatggcc ctggcccatg
     1141 tgcccgaccc cgatctgctg atccgtaccg gcggcgagca gcgcatcagc aacttcctgc
     1201 tgtggcagtg cgcgtacagc gagctgtatt tcaccgactg cctgtggccg gatttcgacg
     1261 ccgccgcgct ggatgcggcg ctggccgcct acgccgggcg cgaacggcgc ttcgggcgca
     1321 cctcggaaca gctggcccag gccggcgcgg ccggttgacc aggggccgac catgctggga
     1381 caacgtgtca tcaccgcgct ggtgctgctg gccgtcctgc tgccggcgct gtttgcgccg
     1441 tcggccacgc ccttcatcgc gctgagtctg ctgttcatcg ctgcggcggc ctgggaatgg
     1501 gcgcgcctga atggcgtggc cggcgccggt gcgctggcca ccggcgcgct gtgcgcggcg
     1561 ctgtgcctgg ggctgtgggc gctgggcgcg gtcgagcgcc cgctgcccgg cctgtggctg
     1621 gcggccgctc tggcctgggt gctgggtggc gcctgggtgc tggcgcgcgg cgtcgccggc
     1681 tgggcgcggg tgccagcggc actgcgctgg gccatgggtc tggcgctcct gagcgtggcc
     1741 tggctggcca tggcgcaggc gcggcgcatc ggcgtcaatt ttttgctgtc ggccatggtg
     1801 ctggtgtggg tggccgacgt gtttgcctac tttgccggcc gggcgctggg cgggcggctg
     1861 accggcggca acaagctggc gcccacgatc agcccgggca agagctggga aggcgtgtgg
     1921 ggcggcatgc tgggggtgct ggcgctggcc gtgctgtggt tgtggctgga tcgcacgcag
     1981 ccgctgggtg cagccagtat ctacacccgc ctgcaccagg ctggaccggc gctgctggtg
     2041 gcgggcgtgc tgtttctgtg cgccatgagc gtggtgggcg atctgttcga atcgctggtc
     2101 aagcgcagcg ccggcatgaa ggactccagc cagttgctgc ccggtcatgg cggcgtgctg
     2161 gaccgcatcg acgcgctgct gcccacgttg ccgctggcca tgctgctggc cagctggtcg
     2221 gccggaggcg gtgcgtgagg cgccagcggc tggcggtgct gggcagcacc ggctcgatcg
     2281 gcaccagcac gctggacgtg gtggcgcgcc accccgagcg cttcgaggtg gtggcgctga
     2341 cggcggccac gcaggtcgat gcgatgctgg cgcagtgcga gcgctttcgc ccgcgcgtgg
     2401 cggtgatggc cagtgccccg cacgccgccg cgttggcgca gaaaatcaaa caaaaaggca
     2461 ttccaacgca ggtggagcaa gcgccggaag ctattgaaaa catagcatgc tggggcgagg
     2521 tagacaccgt catggcggcc atcgtcggcg ccgccggcct ggccccctgc ctggcggcgg
     2581 cgcgcgccgg caagcgcctg ctgctggcca acaaggaggc gctggtggtg ggcggcgagg
     2641 tgtttctgcg cgccgtgcag gccggtggcg ccaccctgct gccgatcgac agcgagcact
     2701 cggccatctt ccagtcgctg ccggaggacc cggccacctg ggcggcgcgc atcgacaaga
     2761 tcatcctcac cgcctcgggc gggccgtttc gcacccgcgc gccggagagc ctcgcggcgg
     2821 tcacgcccga gcaggcctgc gcccatccca actgggtgat ggggcgcaag atctcggtgg
     2881 attcggccac catgatgaac aaggcgctgg aggtgatcga ggcgcgctac ctgttcg
//
LOCUS       DAOOOG010000254         2907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_41776_length_2907_cov_28.612202, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000254 DAOOOG010000000
VERSION     DAOOOG010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41776_length_2907_cov_28.612202"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PK242_12915"
     CDS             <1..485
                     /locus_tag="PK242_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOZ94964.1"
                     /translation="AQKLAEKVKKGDGFDLQDFLGQLQQMKQMGGLSSLMDKLPAQMA
                     AKASDADLTRAEKDIRRKEGIINSMTPAERRKPELLKASRKRRIAQGAGVQVQEVNRL
                     LKEFEQMQGMMKQFKGGALAKMMRRMGGMKGMQGLLGGGAMPPVPPGLGGPGGGKRPF
                     "
     gene            complement(595..1734)
                     /locus_tag="PK242_12920"
     CDS             complement(595..1734)
                     /locus_tag="PK242_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ94965.1"
                     /translation="MNDPLSFNRRRFHTVVGASLVACSRVVRAQDSRIVLGQSAAFSG
                     PAMQLGTQFHAGAKLCFDQLNARGGVNGRPIEIRHLDDGYEPDRCKANTERLIRDEVL
                     ALFGYIGTPTSLAALPLATAAKLPFIAPFSGAMGLREPFHREAFHLRASYDDETELIV
                     RQLTVLGQNRIAVFHQNDAYGQAGLSGVTKALTARQLKPLATATVERNSSDVREAVAR
                     LATSGAQAIVQIGAYQACAAFIREARKSGYGGTFHNVSFVGTQALADALGPDGAGVVV
                     SQVVPSPYDAARPITREFVAATRQSGGKVQANYSSMEGYLAAKVLAEGVRRAGAKLTR
                     DALVTGLESINEDFGGFRVAFGPGKHVASHFAELSMLTGDGRVRT"
     gene            1877..>2907
                     /locus_tag="PK242_12925"
     CDS             1877..>2907
                     /locus_tag="PK242_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOZ94966.1"
                     /translation="MVWLAAGLILAGLGGCTPAGYYAQAVGGHLALLQAARPIDELLA
                     DPASPAELKSRLALARRIRAFAVAELGLPDNASYQRYSDLRRRAAVWNVAAAPPDSLT
                     LRRWCFPVAGCVSYRGYYDEAEARALAERLAREEGLEVRVYGVPAYSTLGWLNWAGGD
                     PLLSTFIRYPEGELARLLFHELAHQQVYVGGDTAFNESYATAVERLGVARWLSSQAGD
                     AARREYAAFDARRQAFRQLVRQARAELAAIYEQNRAQALTESALAAMKSEAMAGFRQR
                     YQALKASWAAAGTPYDAYDPWVAGANNAFFGIQAAYDDGVPAFEALFAREGGDWGRFH
                     AAVQALARLP"
BASE COUNT          448 a          981 c         1045 g          433 t
ORIGIN      
        1 cggcgcagaa gctggccgag aaggtcaaga aaggcgacgg cttcgatctg caggactttc
       61 tgggccagct gcagcagatg aagcagatgg gcggcctgtc cagcctgatg gacaaactgc
      121 cggcgcagat ggccgccaag gccagcgacg ccgacctgac gcgcgccgaa aaagacattc
      181 gccgcaagga aggcatcatc aacagcatga cgcctgccga gcgccgcaag cccgaactgc
      241 tcaaggccag ccgcaagcgc cgcattgccc aaggcgccgg cgtgcaggtg caggaggtca
      301 accgcctgct caaggagttc gagcagatgc agggcatgat gaagcagttc aaaggcggcg
      361 ccctggccaa gatgatgcgc cgcatgggcg gcatgaaggg catgcaaggc ctgctgggcg
      421 gtggcgccat gccgccggtg ccgcctggcc tgggcgggcc ggggggcggc aagcgcccgt
      481 tctgagtcgc caggatctga ggccaatccg gccccggcgc ttgtctgcca agcgccagaa
      541 gctactgatt taatagcgta tggcgatccg gccgagggcc ggaggcgccg cgcttcaggt
      601 gcgcacgcgc ccgtcgccgg tcagcatgga cagctcggca aagtgcgagg ccacgtgttt
      661 gcccggaccg aaggccaccc tgaagccccc gaaatcctcg ttgatcgact ccagtccggt
      721 gaccagcgcg tcgcgcgtga gcttggcgcc ggcgcgccgc acgccctcgg ccagcacctt
      781 ggcggccaga tagccttcca tggacgagta gttggcctgc accttgccgc ccgactggcg
      841 cgtggcggcg acgaactccc gcgttatcgg gcgcgccgcg tcatacggcg agggcaccac
      901 ctgcgacacc accacgccgg cgccgtcggg gcccagggcg tcggccagcg cctgcgtgcc
      961 gacgaacgac acgttgtgga aggtgccgcc atagccggac ttgcgagcct cgcggatgaa
     1021 ggccgcgcag gcctggtagg cgccgatctg cacgatcgcc tgggcgcccg acgtggccag
     1081 cctggccacc gcctcgcgca cgtccgacga gttgcgctcc accgtggcgg tggccagcgg
     1141 cttcaactgg cgcgccgtca gcgccttggt cacgcccgac agcccggcct ggccgtaggc
     1201 gtcgttctgg tggaacacgg cgatgcggtt ctgccccagc accgtgagct ggcgcacgat
     1261 cagctcggtt tcgtcgtcgt aggacgcgcg caggtggaag gcctcgcggt gaaagggctc
     1321 gcgcagtccc atggcgccgc tgaagggcgc gatgaacggc agcttggcgg cggtggccag
     1381 cggcagcgcc gccaggctgg tgggcgtgcc gatgtagccg aacagggcca gcacctcgtc
     1441 gcggatcagg cgctcggtgt tggccttgca gcgatcgggt tcgtagccgt cgtccaggtg
     1501 gcggatctcg atcgggcgcc cgttcacgcc gccgcgggca ttgagctgat cgaagcacag
     1561 cttggcgccg gcgtgaaact gcgtgcccag ctgcatggcc ggcccgctga atgcggccga
     1621 ctggccgagc acgatgcgcg agtcctgcgc gcgcaccacc cggctgcacg ccaccaggct
     1681 ggcgcccacc accgtgtgaa agcggcgtcg gttgaaagac aatggatcgt tcatgagctg
     1741 cttgcccgtc cggcgcgggc cctgtgttgc accatggcac acatcgtaca aaagttccaa
     1801 ttcaagtgca tggggttcgc gatcgaccgt ggcgtggcgg ccaccggggg cgcctgaagt
     1861 gccgccgcgc aggcgcctgg tctggcttgc cgccgggctg atcctggcgg gtttgggtgg
     1921 ctgcacgccg gccgggtact acgcacaggc cgtcggcggt cacctggcgc tgctgcaggc
     1981 ggcgcgcccg atcgacgagc tgctggccga cccggcttcg ccggccgagc tgaagtcgcg
     2041 cctggcgctg gcgcgccgca tccgcgcctt tgccgtcgcc gagctcggtc tgccggacaa
     2101 cgccagctac cagcgctaca gcgatctgcg gcgccgggcc gcggtgtgga acgtggcggc
     2161 ggcgccgccg gactcactga cgctcaggcg ctggtgcttc ccggtcgcag gctgcgtgtc
     2221 ctatcggggt tattacgacg aggccgaggc gcgcgcgctg gccgagcgcc tggcgcgcga
     2281 agaagggctg gaggtgcggg tctacggcgt gcccgcctat tcgaccctgg gctggctgaa
     2341 ctgggccggc ggtgatccgc tgctgtccac cttcatccgc tacccggaag gcgagctggc
     2401 gcgccttctg tttcacgaac tggcacacca gcaggtctat gtcggcggcg acaccgcgtt
     2461 caacgagtcc tacgccaccg cagtcgagcg cctgggtgtg gcgcgctggc tgtcgagcca
     2521 ggccggcgac gcggcccggc gcgagtacgc ggcgttcgac gcccgccgcc aggcgttcag
     2581 gcaactggtg cgccaggcgc gcgccgagct ggccgcgatt tatgaacaaa atcgggctca
     2641 agcgcttacc gaatctgcgc tggcagctat gaaatctgaa gctatggcgg gctttcgtca
     2701 gcgctaccag gcgctcaagg ccagctgggc ggcggccggc acgccgtacg acgcctacga
     2761 cccctgggtg gccggtgcca acaacgcgtt ctttggcatc caggcggcct acgacgacgg
     2821 cgtgccggcc ttcgaggcac tgtttgcccg cgaaggcggc gactgggggc gctttcacgc
     2881 ggcggtgcag gcgctggcca ggctgcc
//
LOCUS       DAOOOG010000255         2898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_41941_length_2898_cov_21.797749, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000255 DAOOOG010000000
VERSION     DAOOOG010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41941_length_2898_cov_21.797749"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PK242_12930"
     CDS             complement(<1..806)
                     /locus_tag="PK242_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HOZ94967.1"
                     /translation="MTRSVADVMKLLQEAECKFVDLRFTDTRGKEQHVTVPVTAFDED
                     KFSDGHPFDSSSVAGWKGIEASDMLLMPDPNTAYVDPFFEEPALVLTCDVLEPGDGKA
                     YERDPRSIARRAEAYLKASGIGDTAYFGPEPEFFVFDSVRWGTEPGHSFYRIEEYEAP
                     WNTGAELEGGNRGHRPRTKGGYFPVPPVDSTHDMRAEMALILDALGVPVEVFHHETAG
                     AGQMEIGTRFSTLVERADWTQRLKYVVWNVADRYGKTATFMPKPYHGDNG"
     gene            complement(969..1784)
                     /locus_tag="PK242_12935"
     CDS             complement(969..1784)
                     /locus_tag="PK242_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOZ94968.1"
                     /translation="MPEFGLIVVGDEILSGKRADKHLPKFVELLTERGLRLAWAEYVG
                     DEPARITATLRRALATGDVVVCTGGIGATPDDHTRQCAAAALGVPLALHPEARRLIEQ
                     RGQDMARQQGQPWEPERADNRQRLNMGVFPAGARIVPNPYNQIPGFALAGPAGGAIYF
                     VPGFPVMAWPMMAWALDTEWSHCFTRGAWRERSVVLYGAMEAPLTPVMERVEAAHPGV
                     RVFSLPSVDHPEHGRHIELGVKGAPAQADAAYADLRAWLQPFALSYGPELVRG"
     gene            complement(1791..2684)
                     /locus_tag="PK242_12940"
     CDS             complement(1791..2684)
                     /locus_tag="PK242_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HOZ94969.1"
                     /translation="MLPRVFDAFLRALGHVFLPRVIGMTLLPLLVMSVVALLLAWLGW
                     QAAVAWMQAALQGWSPLGWAEGWLNIQRPGGLALLLAPLFVVLLATPVIVLASLVVVS
                     LLLTPALTRLVAERRFANLARLGGGSLAASLAWSTGSLLLAVLALVLTLPLWLIPPLV
                     LVLPPLIWGWLTYRVMSFDALVDHASAEERRTILRQHRWPLLLIGVICGYLGAAPGIV
                     WASMALFAVLFVLLVPLAIWIYTFVFVFSSLWFAHYALDALQRLRDQRPPGTAPAAAL
                     PAAQAPDRVTDLPWRDLNGSA"
     gene            complement(2715..>2898)
                     /locus_tag="PK242_12945"
     CDS             complement(2715..>2898)
                     /locus_tag="PK242_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid hydroxylase"
                     /protein_id="HOZ94970.1"
                     /translation="VRGVNFGVLLPCWDALLGSADFSGRCEPTGIRDQVEQGRDYGRG
                     FWAQQWRGLLRLAGRA"
BASE COUNT          457 a         1011 c         1050 g          380 t
ORIGIN      
        1 ctgccgttgt cgccgtggta gggcttgggc atgaaggtgg ccgtcttgcc gtagcggtcg
       61 gccacgttcc agaccacgta tttcaggcgc tgcgtccagt cggcgcgctc gaccagggtg
      121 gaaaagcgcg tgccgatttc catctggccg gcgccggcgg tttcgtggtg gaacacctcc
      181 accggcaccc ccagggcgtc caggatcagc gccatttcgg cgcgcatgtc gtgggtgctg
      241 tcgaccggcg gcaccgggaa atagccgcct ttggtgcgcg ggcggtggcc gcggttgccg
      301 ccttcgagct cggcgccggt gttccagggc gcttcgtatt cctcgatgcg gtagaagctg
      361 tggcccggct cggtgcccca gcgcacgctg tcgaagacga agaattccgg ctcggggccg
      421 aagtaggcgg tgtcgccgat gccgctggcc ttcaggtagg cctcggcgcg gcgcgcgatg
      481 gagcgcgggt cgcgttcgta ggccttgccg tcgccgggct ccagcacgtc gcaggtcagc
      541 accagggcgg gctcctcgaa gaacgggtcg acgtaggccg tgttcgggtc gggcatcagc
      601 agcatgtcgg acgcctcgat gcccttccag ccggcgaccg aggagctgtc gaacgggtgg
      661 ccgtcgctga acttgtcttc gtcgaaggcg gtcacgggca cggtgacgtg ctgctccttg
      721 ccacgggtgt cggtgaagcg caggtcgacg aacttgcatt cggcttcctg cagcagtttc
      781 atgacgtcgg cgaccgatcg ggtcatcggg gtactcctga ggggatgggg atggggatgg
      841 gtgaatggca gagcgccggc cccaggccgg cgtgccgccc gggcgggcgg cgtccggcag
      901 gtggcctgcg cgaaagggtg gattttgcct gttgccgggc ggcaagttcg ccgcgcggcc
      961 tgcgcgcctc agccacgcac cagttctggc ccgtaggaca gcgcaaacgg ctgcagccag
     1021 gcgcgcaggt cggcgtaggc ggcgtcggcc tgcgccggcg cgcccttgac gcccagctcg
     1081 atgtggcgcc cgtgctcggg gtgatcgacg ctgggcaggc tgaacaccct gacgccgggg
     1141 tgcgccgcct ccacccgctc catcaccggc gtcagcggcg cttccatggc gccgtacaac
     1201 accaccgagc gttcgcgcca ggcgccgcgg gtgaagcagt gcgaccattc ggtgtccagc
     1261 gcccaggcca tcatgggcca ggccatgacc gggaaacccg gtacgaaata gatagcacct
     1321 cccgcaggcc ccgcaagcgc aaaacccgga atctggttgt aagggttggg cacgatgcgg
     1381 gcccctgcgg ggaacacgcc catgttcagc cgctggcggt tgtcggcgcg ctcgggctcc
     1441 cagggctggc cctgctggcg cgccatgtcc tggccgcgct gctcgatcag gcgccgggcc
     1501 tcggggtgca gggccagcgg cacccccagg gcggcggcgg cgcactggcg cgtgtggtcg
     1561 tcgggcgtcg cgccgatgcc gccggtgcac accaccacgt cgcccgtggc cagggcgcgc
     1621 cgcagcgtgg cggtaatgcg cgccggttcg tcgcccacgt attcggccca ggccaggcgc
     1681 aggccgcgct cggtcagcag ctcgacgaac ttgggcaggt gcttgtcggc acgcttgccg
     1741 gagaggattt cgtcaccgac gacgatgagg ccgaattcgg gcatggggtt tcaggcagaa
     1801 ccgttgagat cgcgccacgg caggtcggtc acgcggtcgg gtgcttgcgc cgccggcagc
     1861 gccgccgccg gcgcggtgcc cggcgggcgc tggtcgcgca ggcgctgcag cgcgtcgagc
     1921 gcatagtgcg cgaaccacag cgaggaaaac acgaagacga aggtgtagat ccagatcgcc
     1981 agcggcacca gcagcacgaa cagcacggca aacagcgcca tcgaggccca gacgatgccc
     2041 ggggcggcgc ccaggtagcc acagatcacg ccgatcagca gcagcggcca gcggtgctgg
     2101 cgcaagatgg tgcggcgttc ttcggcgctg gcgtggtcga ccagggcgtc gaagctcatc
     2161 acccggtagg tcagccagcc ccagatgagc ggcggcagca ccagcaccag cggcggaatc
     2221 agccacagcg gcagcgtcag caccagcgcc agcacggcca gcagcagcga gccggtcgac
     2281 cacgccaggg aagcggccag cgagcccccg cccagccgcg ccaggttggc aaagcgccgt
     2341 tcggccacca gccgcgtgag cgccggtgtc agcagcagcg acaccaccac cagcgacgcc
     2401 agcacgatca ccggcgtggc cagcaggacc acgaacagcg gcgccagcag cagcgccagg
     2461 ccgcccggcc gctggatgtt cagccagccc tcggcccagc ccagcggcga ccagccctgc
     2521 agcgccgcct gcatccaggc caccgccgcc tgccagccca gccacgccag cagcagcgcc
     2581 accacgctca tcaccagcag cggcagcagc gtcatgccga tgacgcgcgg cagaaacacg
     2641 tggcccaagg cacgcaggaa ggcgtcgaac acgcggggca gcatggcgtc aggatagcgc
     2701 ggcgcgccgc ccgctcaggc gcgcccggcc aggcgcagca ggccccgcca ctgctgggcc
     2761 cagaagccgc gcccgtagtc gcgcccctgc tcgacctgat cgcggatgcc ggtgggctcg
     2821 cagcggccgg aaaagtcggc cgagcccagc agcgcgtccc agcacggcag cagcacgccg
     2881 aagttgacgc cccggaca
//
LOCUS       DAOOOG010000256         2878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_42351_length_2878_cov_23.883457, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000256 DAOOOG010000000
VERSION     DAOOOG010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42351_length_2878_cov_23.883457"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..2021
                     /locus_tag="PK242_12950"
     CDS             354..2021
                     /locus_tag="PK242_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94971.1"
                     /translation="MPRSNQKMQMLSALCAATLLTLTACGGDGDDSVAGPAPAPSPAP
                     GPAPVTLQGQVTRNAALKDATVCLDLNGNDACDAGEPVSALTDANGQYSLTALPAQAA
                     AARLIAVVKANVTVDANKPAQPVTTTTDYVLKRPAGSAGGINPLTTLVQAGVAAGMSE
                     AQARSNVAAQLGIAAAKIDDYQADPPANDTQVQDTARWIAAFTSTALRDGIALEVADP
                     SAAEPASDVMDNLIWADASNFYWRSLQQAARPAGAPTPAVADMRAGKIGGTARPDFGA
                     ANSLYRTAYLTPAGWQMCGRNTPPIVSSGGNPSRSLYCGTSAAVTIHRRSDVAGEAMS
                     ALVTRWQGDAVTNRINNDGTPVAALNGALGATVFPAGAQEILRRGLTLTDDILIDNTW
                     TRGVPQSVATTLAQLVTAYPVASVNISDGPNAGNTTRSLGLGSATNKNMRVAFGPTAG
                     AAQFYECDLDASGNFATPPNCVATTAGTYTIETVHGVKVMRFAGHPAVVNTINYQVVY
                     AEVDWGGAGNQWVYRAHATKPDWSSRYATTMRLNTTAWSAMKTQLGL"
     gene            2083..2466
                     /locus_tag="PK242_12955"
     CDS             2083..2466
                     /locus_tag="PK242_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94972.1"
                     /translation="MKTLSLCLLAVAGVLVGCASAPPAQTEFPPGARAPTAAEAVSLL
                     RGKSFTVANPNGSSTRVDHAADDSGGLTAYFAGRTDSGTWRAEDGRVCYEFKVIPSAC
                     NDMRLVGAAIYIQRSNGQVVQLVPR"
     gene            complement(2544..>2878)
                     /locus_tag="PK242_12960"
     CDS             complement(2544..>2878)
                     /locus_tag="PK242_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOZ94973.1"
                     /translation="DWSEEYLAPIISIKVVAGLEEAIAHINRYSSHHTDAILTTDHRH
                     AQRFLREVDSASVMVNTSTRFADGFEYGLGAEIGISTDKFHARGPVGIEGLTSLKWVV
                     LGEGEVRG"
BASE COUNT          474 a         1022 c          957 g          425 t
ORIGIN      
        1 accaacccat ggatcagctg accggtgttt tgaaaaattc acaagctctc agcccacatg
       61 ggcgcacctg cgcgcgctcg tcggcgcgga tcggccaggc accacagcgc gggtccatcg
      121 ctggggcggg cgaggcgggg caaagtggtt catggcgggt ggcagtgcag gtgcgaacgg
      181 cgcgcgggca tgcaagcgta tgcgggttga accgcacgca caagccggcc ggtatcccat
      241 gttcatggca tgtgcgtcac gcagtccggt tcgatagttc ggccgtccgc ccggcctgtc
      301 ctatggcggc ggatggcacc ttgacccaca ccctttcgga ggaggaaccc cacatgcccc
      361 gttccaacca gaagatgcag atgctcagcg cactgtgcgc ggccacgctg ctgaccctca
      421 ccgcatgcgg tggcgatggc gacgattcgg tcgccggccc cgcgccagcg ccgagtcctg
      481 ccccagggcc agcccctgtg acgctgcaag gccaggtcac tcgcaacgcc gcgctgaagg
      541 acgccacggt ctgcctggac ctcaacggca acgacgcctg cgacgccggc gagccggttt
      601 cggcgctcac cgatgcgaac ggtcagtact cactcacggc tttgccggct caggcggccg
      661 ccgcgcgcct gatcgccgtg gtcaaggcca acgtgacggt cgacgccaac aagccggccc
      721 agccggtgac cactacgacc gactacgtgc tcaagcgccc ggccggctcg gctggcggca
      781 tcaacccgct caccaccctg gtgcaggccg gtgtggccgc cggcatgagc gaagcgcagg
      841 cgcgcagcaa cgtggcggcc cagctgggca ttgcggccgc caagatcgac gactaccagg
      901 ccgatccgcc cgccaacgac acccaggtgc aggacacggc gcgctggatt gccgccttca
      961 cctcgaccgc gctgcgcgac ggcattgcgc tggaggtcgc cgatccgtcg gcggccgaac
     1021 cagcctccga cgtgatggac aacctgatct gggccgacgc cagcaacttc tactggcgca
     1081 gcctgcagca ggccgcccgg ccggccggcg cgccgacccc ggcggtggcc gacatgcgcg
     1141 ccggcaagat cggcggcacc gcccggcccg acttcggcgc cgccaacagc ctgtaccgca
     1201 cggcctacct cacgcccgcc ggctggcaga tgtgcgggcg caacacgccg cccattgtct
     1261 ccagcggcgg caatccaagc cgctcgctgt actgcggcac ctcggccgcc gtcacgatcc
     1321 accggcgcag tgacgttgcc ggcgaggcca tgtcggcgct ggtgacgcgc tggcagggcg
     1381 atgcggtgac caaccgcatc aacaacgacg gcacgccggt cgccgcgctc aatggcgcgc
     1441 tgggggccac cgtgttcccc gcgggcgcgc aggaaatcct gcgccgtggc ctgacgctga
     1501 cggacgacat cctgatcgac aacacctgga cgcgcggggt gccgcagtca gtcgccacca
     1561 cgctggcgca gttggtgacg gcctatccgg tggccagcgt caacatctcc gatggcccca
     1621 acgccggcaa caccacccgc agtctcggcc tgggcagcgc gaccaacaag aacatgcgcg
     1681 tggcgttcgg gccaacggct ggcgcggcgc agttctatga atgtgacctg gatgccagcg
     1741 gcaacttcgc cacgccgccc aactgcgtgg cgaccacggc gggcacctac accatcgaga
     1801 ctgtccacgg cgtcaaggtc atgcgctttg ccggccaccc ggcggtcgtg aacaccatca
     1861 actaccaggt ggtctatgcc gaagtcgatt ggggcggcgc cggcaaccag tgggtgtacc
     1921 gagcgcatgc caccaagccg gactggtcgt cacgctacgc aaccaccatg cggctcaaca
     1981 ccacggcctg gagcgcgatg aagacccagc tgggcctgtg agtgcccacg ccaccccggg
     2041 cccgcgcgcc cggggcatgg attccaagac gaaggagttg acatgaaaac cctctcactt
     2101 tgcctgctgg ccgttgccgg cgtgctggtc ggctgcgcct cggcgccgcc tgcgcagacc
     2161 gagtttccgc ccggcgcgcg cgcgcccacc gcggccgagg ccgtgagtct gctgcgcggc
     2221 aagtcgttca ctgtggccaa tcccaatggc tccagcaccc gcgtcgacca cgcggccgat
     2281 gacagcggtg gcctgaccgc gtactttgcg ggtcgcaccg acagcggcac ctggcgcgcc
     2341 gaggacggtc gggtctgcta tgagttcaag gtcatacctt cggcctgcaa cgacatgcgg
     2401 ctggtcgggg cggccatcta tatccaacgc agcaacggtc aggtggtgca actggtgccg
     2461 cgctgagagc cgtcggatcg gcccatctcc agggagttgc cggccggcgt cgcaaggcgc
     2521 cggccttttt atgtgcccac cgttcagccg cgcacctcgc cctcgcccag caccacccac
     2581 ttgagcgagg tcagaccctc gatgccgacc ggcccgcggg cgtggaattt gtcggtgctg
     2641 atgccgatct cggcgcccag gccgtattcg aagccgtcgg caaagcgggt gctggtgttg
     2701 accatgacgc tggccgaatc gacctcgcgc agaaagcgct gggcgtggcg gtggtcggtg
     2761 gtgaggatgg cgtcggtgtg gtggctggag tagcggttga tgtgggcgat ggcttcttcc
     2821 agcccggcca ccaccttgat gctgatgatg ggcgccaggt attcctcgga ccagtcgg
//
LOCUS       DAOOOG010000257         2841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_43137_length_2841_cov_25.511486, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000257 DAOOOG010000000
VERSION     DAOOOG010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43137_length_2841_cov_25.511486"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2113
                     /locus_tag="PK242_12965"
     CDS             <1..2113
                     /locus_tag="PK242_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOZ94974.1"
                     /translation="QELEDLYLPYKPKRRTKGQIAREFGLEPLADRLWGDPTLDPARE
                     AQGFTRAEKGEGGEDFGTVQAVLDGVRDILSERWAEDAALVQSLREWLWADGLLQSKL
                     AAGRNEADAEVAKFRDYFDYDEPIARVPSHRALAVFRGRQLEILDVKLVIPDAEPAPA
                     ATKTVASIGLAEGRIALHLGWSHQGRAADDLLRRCVVWTWRVKLQLSTERELLARLRE
                     DAEQVAIKVFGDNLRDLLLAAPAGHKVVMGLDPGIRTGVKVAVVDATGKLLATATVYP
                     HEPRRDWDGSLHTLAGLVARHGVQLIAIGNGTASRETDRLAGDLIKLVKKQAAALAGQ
                     APAAIEKIVISEAGASVYSASEYASQELPDVDVSLRGAASIARRLQDPLAELVKIDPK
                     AIGVGQYQHDVNQSALARQLDAVVEDCVNGVGVDLNTASAPLLARVSGLSPAVARAVV
                     RWREQHGAFRSRQQLLEVSGLGPKTFEQSAGFLRIRGGDNPLDVTGVHPETYPLVEQI
                     ILKAGRPIEQLMGRAELLRTLRPEQFVTEAFGAVTVRDILVELEKPGRDPRPDFKVAR
                     FNEGVEDIADLKPGMVLEGTVSNVAAFGAFVDLGVHQDGLVHVSQLAHKFVQDAREVV
                     KTGQIVKVKVLEVDAARQRISLTMKLDAAAPRRDAPRDNRFEPAGRPARPGHAAGRER
                     GSAAATAMASAFEKLRGQQAK"
     gene            complement(2218..>2841)
                     /locus_tag="PK242_12970"
     CDS             complement(2218..>2841)
                     /locus_tag="PK242_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOZ94975.1"
                     /translation="RMQAHEALGRSVSLLADDRGVLALFAVADALKPDTPAAVQQLRA
                     LGVEPIMLTGDNPTTAQAIAAQAGIGDVRAGLLPQHKLEAITALNAHSPGTVGMVGDG
                     INDAPALAAAALGFAMGQGGTHVASEAADVVIMNDDLRRVPETVRLSRRTHAILWQNI
                     VLALGIKAVFLLLAVLGSATMWMAVFADMGASLLVVFNGLRMLRWRG"
BASE COUNT          471 a          977 c         1016 g          377 t
ORIGIN      
        1 gcaggagctg gaagacctgt acctgcccta caaacccaag cgccgcacca agggccagat
       61 cgcgcgcgaa ttcggcctcg aaccgctggc cgaccgcctg tggggcgacc cgacgctgga
      121 ccccgcccgt gaggcgcagg gtttcacccg ggcggaaaaa ggcgagggcg gggaggactt
      181 cggcaccgtg caggcggtgc tggacggcgt gcgcgacatc ctgagcgagc gctgggccga
      241 agacgccgcc ctggtgcagt cgctgcgcga atggctgtgg gccgacggcc tgctgcagag
      301 caagctggcc gccggcagga acgaggccga tgccgaggtg gccaagtttc gcgactactt
      361 cgattacgac gagccgatcg cccgcgtgcc cagtcaccgg gcgctggcgg tgtttcgcgg
      421 ccgccagctc gaaatactgg atgtgaaact ggtgattccc gacgcggagc ctgcgccagc
      481 agctaccaaa accgtagcat cgatcgggct ggccgaaggg cggatcgccc tgcatctggg
      541 ctggtcgcac cagggccgcg ccgccgacga cctgctgcgt cggtgcgtgg tctggacctg
      601 gcgcgtcaag ctgcaactgt ccaccgaacg cgagctgctg gcccgcctgc gcgaagacgc
      661 ggagcaggtg gcgatcaagg tgttcggcga caacctgcgc gacctgttgc tggccgcgcc
      721 ggccggccac aaggtcgtga tggggctgga cccgggcatc cgcaccggcg tcaaggtggc
      781 ggtggtcgac gccaccggca agctgctggc aaccgccacc gtctatccgc acgagccgcg
      841 gcgcgactgg gacggcagcc tgcacaccct ggcgggcctg gtcgcgcggc acggggtgca
      901 gctgatcgcg atcggcaacg gcaccgccag ccgcgaaacc gaccggctcg ccggcgacct
      961 catcaaactg gtgaaaaaac aggctgcagc gcttgctggg caagcgccag cagctatcga
     1021 aaagatagtt atcagcgaag ccggtgcctc cgtctacagc gccagcgaat acgccagcca
     1081 ggagctgccg gacgtcgacg tgagcctgcg cggcgccgcc agcattgcgc gtcgcctgca
     1141 ggacccgctg gccgagctgg tgaagatcga ccccaaggcg atcggcgtcg gccagtacca
     1201 gcacgacgtc aaccagagcg cgctggcgcg ccagctcgac gcggtggtgg aagactgcgt
     1261 caacggcgtc ggcgtggacc tgaacaccgc cagcgcgccg ctgctggcgc gggtgtcggg
     1321 cctgagcccg gcggtggcgc gcgccgtggt gcgctggcgc gagcagcacg gggccttcag
     1381 gagccgccag cagttgctgg aagtcagcgg cctggggccc aagaccttcg agcagagcgc
     1441 cggctttctg cgcatccgcg gcggcgacaa cccgctggat gtgaccggcg tgcaccctga
     1501 aacctatccg ctggtcgagc agatcatcct gaaggccggg cggccgatcg agcagctgat
     1561 ggggcgtgcc gagctgctgc gcacgctcag gccggagcag ttcgtcaccg aggcgttcgg
     1621 cgccgtcacc gtcagggaca tcctggtcga gctggaaaaa cccggccgcg atccgcgccc
     1681 cgacttcaag gtggcgcgct tcaacgaggg cgtggaagac atcgccgatc tgaagcccgg
     1741 catggtgctg gaaggcaccg tcagcaacgt ggccgccttc ggcgccttcg tcgacctggg
     1801 cgtgcaccag gacgggctgg tgcatgtgag ccagctggcc cacaagttcg tgcaggacgc
     1861 gcgcgaggtg gtcaagaccg gtcagatcgt gaaggtcaag gtgctggagg tggacgcggc
     1921 gcgccagcgc atcagcctga cgatgaagct ggacgccgcc gcgccgcgcc gtgacgcgcc
     1981 gcgcgacaac cgcttcgagc ccgccgggcg gccggcgcgc ccgggccacg cggcagggcg
     2041 cgaacgcggc tcggcggccg ccaccgccat ggcctcggcg ttcgagaaac tgcgcggcca
     2101 gcaagccaaa tagccttgta gcgcgcttgc agcatgcgcc agcagctatc gaaatggaag
     2161 tgaacgggct cgggccaggg cggtcaccaa aggcatcagg gcttggttca caccgcctca
     2221 ccccctccag cgcagcatgc gcagaccgtt gaacaccacc agcagcgacg cccccatgtc
     2281 ggcgaacacc gccatccaca tggtggcgct gcccagcacc gccagcagca gaaacaccgc
     2341 cttgatgccc agcgccagga cgatgttctg ccacaggatg gcgtgcgtgc ggcgcgacag
     2401 gcgcacggtt tcgggcacgc ggcgcaggtc gtcgttcatg atcaccacgt cggcggcctc
     2461 gctggccacg tgcgtgccgc cctgacccat ggcaaagccc agcgccgcgg cggccagcgc
     2521 cggggcgtcg ttgatgccgt cgcccaccat gcccacggtg ccagggctgt gcgcgttcag
     2581 cgccgtgatg gcctccagct tgtgttgcgg cagcaggccg gcgcgcacgt cgccgatgcc
     2641 ggcctgagcg gcgatggcct gcgcggtggt cgggttgtcg ccggtcagca tgatcggctc
     2701 cacccccagc gcgcgcagct gctgcacggc ggcgggcgtg tcgggcttga gtgcgtcggc
     2761 cacggcgaac agcgccagca cgccacggtc gtcggccagc aggctgacgc tgcggcccag
     2821 cgcctcgtgc gcctgcatgc g
//
LOCUS       DAOOOG010000258         2840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_43165_length_2840_cov_23.308797, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000258 DAOOOG010000000
VERSION     DAOOOG010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43165_length_2840_cov_23.308797"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PK242_12975"
     CDS             <1..991
                     /locus_tag="PK242_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOZ94976.1"
                     /translation="VLVYTLYGGMWSVALTDFLQMIVITAGLLAIAWYAGDMAGGADK
                     VVAYAAREGKFNFLPTGGLKEWVFFFAAGITMMLGSIPQQDVFQRVMSSRDVKTAQRG
                     PVIGGLLYIAFAFVPMFIVMAALLVMPETAALLDDDPQKVLPTLVMGHMPVLLQVAFF
                     GALLSAIMSTASATLLAPATTFVENILRNLRPGMNDAETLKAMRISVLVFTLCVLVYS
                     ITMEGSSIYELVSGAYQVPLVGAFVPLAFGVYWRRATTQGALLAVVMGIGVWVLFVAS
                     PMLNEAFPQQLAGVLAAIVGMVFGSLAPQWLPNHKGYVHHLVEREAVTGEPAR"
     gene            1097..1396
                     /locus_tag="PK242_12980"
     CDS             1097..1396
                     /locus_tag="PK242_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOZ94977.1"
                     /translation="MPIYAYKCAACGFAKDVLQKMSDPPLAECPSCGQAAFEKQLTAA
                     GFQLKGSGWYVTDFRDGGSKGGAAAPSPAPAAAQAAAPAPAAPAPASSSPAPAGS"
     gene            1418..2137
                     /locus_tag="PK242_12985"
     CDS             1418..2137
                     /locus_tag="PK242_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOZ94978.1"
                     /translation="MMSRLRQYFITGLLVWLPMGVTVWVLLWLLGILDGIFLGVLSAA
                     EAMVPGLGPLANRLRHVPGLGVILVAIVIFSTGVFVANMFGQWSVRQWDRLMTRIPVV
                     RSIYSGVKQVSDTLFSGNGQAFSKALLIQYPRPGAWTIAFVTGKPEGEVSCHLPPDCI
                     SVYVPTTPNPTSGFFLMVPRADAIELRMSVDEALKYVISMGVVAPPQRQPCPPGEAVP
                     EPLQAQVVSAPADARQSPPAG"
     gene            2176..>2840
                     /locus_tag="PK242_12990"
     CDS             2176..>2840
                     /locus_tag="PK242_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOZ94979.1"
                     /translation="MSMRTHYCGLVTEALIGQTVSLCGWVNRRRDHGGVIFVDLRDRE
                     GYVQVVCDPDRPGMFSVAEDLRNEFCIQVKGLVRARPEGTSNDNLKSGKIEVLCHELV
                     VLNPSVTPPFQLDDDNLSETTRLTHRVLDLRRPAMQRNLMLRYKTAIQVRNFLDKEGF
                     IDIETPMLGKSTPEGARDYLVPSRVHDGMFFALPQSPQLYKQMLMVAGYDRYYQITKC
                     FRD"
BASE COUNT          428 a          985 c          925 g          502 t
ORIGIN      
        1 ggtgctggtc tacacgctgt atggcggcat gtggtcggtg gcgctgaccg atttcctgca
       61 aatgatcgtc atcaccgccg gtctgctggc catcgcctgg tacgccggcg acatggccgg
      121 cggtgccgac aaggtggtgg cctacgccgc acgcgaaggc aagttcaact tcctgcccac
      181 tggcggcctg aaggaatggg tgttcttctt tgccgccggc atcaccatga tgctgggctc
      241 cattccgcag caggacgtgt tccagcgcgt gatgtcctca cgcgacgtca agaccgccca
      301 gcgcgggccg gtgatcggcg ggctgctgta catcgccttc gccttcgtgc cgatgttcat
      361 cgtcatggcc gcgctgctgg tgatgcccga gaccgcggcg ctgctggacg acgacccgca
      421 gaaggtgctg cccacgctgg tgatgggcca catgccggtg ctgctgcagg tggccttctt
      481 cggcgccctg ctgtcggcca tcatgtccac cgcctcggcc acgctgctgg cgccggccac
      541 caccttcgtc gaaaacatcc tgcgcaacct gcgcccgggc atgaacgacg ccgaaaccct
      601 gaaggccatg cgcatcagcg tgctggtgtt caccctgtgc gtgctggtct attcgatcac
      661 catggaaggc tcgtccatct acgagctggt ctcgggcgcc taccaggtgc cactggtggg
      721 cgccttcgtg ccgctggcgt ttggcgtcta ctggcggcgc gccaccaccc agggcgcgct
      781 gctggcggtg gtgatgggca tcggcgtgtg ggtgctgttc gtggccagcc cgatgctgaa
      841 cgaggccttc ccacagcagc tggccggcgt gctggcggcg atcgtcggca tggtgttcgg
      901 ttcgctggcg ccgcaatggc tgcccaacca caagggctac gtccaccacc tggtggaacg
      961 cgaggccgtc acgggcgagc cggcgcgctg acgcaccgca gggggcacca caggggcgtc
     1021 gcagccacgg cggcgtggca agggccgaac ttttataatg aaaagttttt tgccccagcc
     1081 tgatttcgcg ttcgccatgc ccatctacgc ctacaagtgc gccgcctgcg gttttgccaa
     1141 ggacgtgctg cagaagatgt ccgacccgcc cctggccgag tgcccgtcct gcggacaggc
     1201 ggcgttcgag aagcagctca ccgccgccgg ctttcagctc aagggctcgg gctggtacgt
     1261 gaccgatttc cgcgacggcg gctccaaagg gggcgcggcc gcgccgtcgc ccgcacccgc
     1321 ggcggcgcaa gccgcggcgc ccgcgcccgc cgccccggcc cccgccagca gcagcccagc
     1381 gcccgccggc agctgatcct caagagaacc gcatcccatg atgtcccgcc tgcggcaata
     1441 cttcatcacc ggcctgctgg tgtggctgcc catgggcgtg acggtgtggg tgctgctgtg
     1501 gctgctcggc atcctggatg gcatcttcct gggcgtgctg tcggcggccg aagccatggt
     1561 gccgggcctg gggccgctgg ccaaccgact gcgccacgtg cccggtctgg gtgtgatcct
     1621 ggtggccatc gtcatcttca gcaccggcgt gttcgtggcc aacatgttcg gccagtggtc
     1681 ggtgcggcag tgggaccggc tgatgacgcg cattccggtg gtgcgcagca tctattccgg
     1741 cgtcaagcag gtgtccgaca ccctgttttc aggcaacggt caggcctttt ccaaggcgct
     1801 gctgatccag tacccgcgcc ccggtgcctg gaccatcgcc ttcgtcaccg gcaagccgga
     1861 gggcgaggtc agctgccacc tgccgcccga ctgcatcagc gtctatgtgc cgaccacgcc
     1921 caaccccact tcgggcttct tcctgatggt gccgcgggcc gacgccatcg agctgcgcat
     1981 gagcgtggac gaggcgctga agtacgtgat ttccatgggc gtggtggcgc cgccccagcg
     2041 ccagccgtgt ccacccggcg aggccgtgcc cgagccgctg caggcgcagg tcgtcagcgc
     2101 tcccgccgat gcccggcaat cacctcctgc cggctgacct ggccggccct gaaccgtttt
     2161 ccgaaagtcc attccatgtc gatgcgtacc cactactgcg gcctcgtgac cgaggccctg
     2221 atcggtcaga ccgtgagcct gtgcggctgg gtcaaccgcc ggcgcgacca cggcggcgtg
     2281 atcttcgtcg atctgcgcga tcgtgaaggc tatgtgcagg tggtgtgcga ccccgaccgg
     2341 cccgggatgt tcagcgttgc cgaggacctg cgcaacgagt tctgcatcca ggtcaagggc
     2401 ctggtgcgcg cgcgccccga gggcaccagc aacgacaacc tcaagagcgg caagatcgag
     2461 gtgctgtgcc acgagctggt ggtgctcaac ccctcggtca cgccgccgtt ccagctcgat
     2521 gacgacaacc tgtcggaaac cacccgcctg acgcaccgcg tgctcgacct gcgccgcccg
     2581 gccatgcagc gcaacctgat gctgcgctat aagaccgcga tccaggtgcg caacttcctc
     2641 gacaaggaag gcttcatcga catcgaaacc cccatgctcg gcaagagcac gcccgagggc
     2701 gcgcgcgact acctggtgcc cagccgcgtg cacgacggca tgttcttcgc gctgccgcag
     2761 tcgccccagc tgtacaagca gatgctgatg gtggccggct acgaccgcta ctaccagatc
     2821 accaagtgct tccgcgacga
//
LOCUS       DAOOOG010000259         2827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_43453_length_2827_cov_21.299062, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000259 DAOOOG010000000
VERSION     DAOOOG010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43453_length_2827_cov_21.299062"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1253
                     /locus_tag="PK242_12995"
     CDS             15..1253
                     /locus_tag="PK242_12995"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HOZ94980.1"
                     /translation="MTTTDPGARPLPEGLHPDTLAVRTGIAPSAYGESSEALYLTSGF
                     VQPDADTSARRFAHPEQGYTYSRTGNPSVTAFERRLAALEGAEAAIGTASGMAAILLM
                     GLGLLKSGDHVVCSRSMFGSTLRLLGQEFARFGVETSFVSQTDVAEWRAAVRPGTRLL
                     FAETPTNPLTEVCDIAALAAIAHGVGARLAVDNCFATPVLQRPLALGADLVIHSGTKF
                     LDGQGRVMAGAICGSRELIEGVFAPLMRTAGMVLAPFNAWVVHKGMETLAVRVRAQSD
                     HALTLARWLERHPAVERVFYPGLPSHPQHALAMAQQAGVGGAVLAFSVRADAPEQARA
                     RAFHVIDSTRVCSIATNLGDVKTLICHPASTSHGRLSEDQRQAAGVVQGLIRLAVGLE
                     HPGDIQDDLARGLDGCRNTT"
     gene            1308..2714
                     /gene="gltX"
                     /locus_tag="PK242_13000"
     CDS             1308..2714
                     /gene="gltX"
                     /locus_tag="PK242_13000"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOZ94981.1"
                     /translation="MTEKSTPAARVRTRFAPSPTGFIHLGNIRSALYPWAFARSQGGD
                     FILRIEDTDLERSTQAAVDVILEGMAWLGLDHDEGPFYQMQRMDRYREVLAQLQAAGH
                     VYPCYMSVAELDALRERQMARKEKPRYDGTWRPERGKTLPPVPAGVQPVLRFRNPQDG
                     VVAWDDKVKGRIEVHNSELDDLVIARPDGTPTYNFCVVVDDLDMRITHVIRGDDHVNN
                     TPRQINIFRALGHEPPVYAHLPTVLNEQGEKMSKRNGAKPVTQYRDEGFLPDAMVNYL
                     ARLGWSHGDDEIFSRAQFLAWFDLEHLGRSAAQFDEAKLRWVNAQHLKAMDDAALAPL
                     VAAQLHQRGVTPDERLPALCGLFKDRCDTTRALADWLMPYHTDITPPADALAAHLTDA
                     VRPAIAAFADRLQAADWSAESLGAALRQTLAEHGLKMPQLAMPVRVLVMGTAQTPSVD
                     AMLALHRRDTVLARLRAA"
BASE COUNT          454 a         1027 c          937 g          409 t
ORIGIN      
        1 ctgagggatg ccgcatgacg acaactgatc ctggcgcccg cccgctgccc gagggcctgc
       61 accccgacac cctggcggtg cgcaccggca ttgcgcccag cgcctatggc gagagctccg
      121 aggcgctgta cctgaccagc ggattcgtgc agccggacgc cgacacctcg gcccgccgct
      181 ttgcccaccc ggagcagggc tacacctatt cgcgcaccgg caacccctcg gtgaccgcct
      241 tcgagcgccg cctggccgcg ctggaaggcg ccgaagccgc catcggcacc gccagcggca
      301 tggccgccat cctgctgatg ggcttgggcc tgctgaaaag cggcgaccac gtggtgtgct
      361 cgcgctccat gttcggctcc acgctgcggc tgctgggcca ggaattcgcg cgcttcgggg
      421 tcgaaaccag tttcgtgtcg cagaccgacg tggccgaatg gcgggccgcc gtgcgccccg
      481 gtacccgcct gctgttcgcc gaaaccccca ccaacccgct gaccgaggtg tgcgacatcg
      541 ccgcgctggc ggccatcgcc catggcgtgg gcgcgcggct ggcggtggac aactgctttg
      601 ccaccccggt gctgcagcgg ccgctggcgc tgggggccga tctggtgatc cattcgggca
      661 ccaagttcct cgacggtcag gggcgtgtga tggctggcgc catctgtggc tcgcgcgagc
      721 tgatcgaagg cgtgttcgcg ccgctcatgc gcaccgccgg catggtgctg gcgccgttca
      781 acgcctgggt ggtgcacaag ggcatggaga cgctggcggt gcgggtgcgc gcccagagcg
      841 accacgcgct gaccctcgcc cgctggctgg agcggcaccc ggcggtcgag cgcgtgttct
      901 accccggcct gccctcgcac ccgcagcacg cgctggcgat ggcgcagcag gcgggcgtgg
      961 gtggtgcggt gctcgccttc agcgtgcgcg ccgacgcgcc cgagcaggca cgcgcgcggg
     1021 ccttccacgt catcgattcg acccgcgtgt gcagcatcgc caccaacctg ggcgacgtca
     1081 agacgctgat ctgtcacccg gccagcacct cgcacgggcg cctgagcgag gatcagcggc
     1141 aggccgccgg cgtggtgcag ggcctgatcc ggctggcggt cggcctggag cacccgggcg
     1201 acatccagga cgacctggcg cgcggcctgg acgggtgccg gaacaccacc tgattgctat
     1261 gtaaatgata gctgcttgcg cttactgggc aagcgccaag gcaagatatg actgaaaaat
     1321 caacccctgc tgcgcgcgta cgcacccgct ttgccccatc gcccaccggc ttcattcacc
     1381 tgggcaacat ccgctcggcc ctgtacccct gggcctttgc gcgctcacag ggcggcgact
     1441 tcattctgcg catcgaagac accgacctgg aacgctccac ccaggccgcg gtcgacgtca
     1501 tcctcgaagg catggcctgg ctcgggctcg accacgacga ggggccgttc taccagatgc
     1561 agcgcatgga ccgctaccgc gaagtgctgg cgcagctgca ggccgccggc catgtctacc
     1621 cctgctacat gagcgtggcc gagctcgacg cgctgcgtga gcgccagatg gcgcgcaagg
     1681 aaaaaccccg ctacgacggc acctggcgcc ccgagcgcgg caagaccctg ccgcccgtgc
     1741 ccgccggcgt gcagccggtg ctgcgctttc gcaacccgca ggacggcgtg gtggcctggg
     1801 acgacaaggt caagggccgc atcgaggtgc acaacagcga actcgacgac ctggtcatcg
     1861 cccggccgga cggcaccccc acctacaact tctgcgtcgt ggtcgacgac ctcgacatgc
     1921 gcatcaccca cgtgatccgc ggcgatgacc acgtcaacaa cacgccgcgg cagatcaaca
     1981 tcttccgcgc cctgggccac gaaccgcccg tctacgcgca cctgcccacc gtgctgaacg
     2041 agcagggcga gaagatgagc aagcgcaacg gcgccaagcc cgtcacccag taccgcgacg
     2101 agggctttct gcccgacgcc atggtcaact acctggcgcg cctgggctgg agccatggcg
     2161 acgacgagat cttctcgcgt gcgcagttcc tggcctggtt cgacctcgaa cacctgggcc
     2221 gcagcgccgc ccagttcgac gaggccaagc tgcgctgggt caacgcccag cacctgaagg
     2281 ccatggacga cgcggcgctg gcgccgctgg tggcggcaca actgcaccag cgtggcgtga
     2341 cgcccgacga acgcctgccc gcgctgtgcg gcctgttcaa ggaccgctgc gacaccacgc
     2401 gggcgctggc cgactggctg atgccctacc acaccgacat cacccccccg gccgatgcgc
     2461 tggcggcgca cctgaccgac gccgtgcgtc cggccatcgc cgcctttgcc gaccggctgc
     2521 aggcggccga ctggagcgct gaaagtcttg gcgctgcgct ccggcagacc ctggccgagc
     2581 acggcctgaa gatgccgcaa ctggccatgc cggtgcgcgt gctggtcatg ggtacggcgc
     2641 aaacgccctc ggtcgacgcc atgctggcgc tgcatcggcg cgacaccgtg ctggcccgct
     2701 tgcgcgcggc ctgaatttcg atatacaatg caaggctcga cacccacgac ggggaagccc
     2761 atcgaggaaa ctcaacgggg gtatagctca gctgggagag cgcttgcatg gcatgcaaga
     2821 ggtcagc
//
LOCUS       DAOOOG010000260         2788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_44345_length_2788_cov_22.287962, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000260 DAOOOG010000000
VERSION     DAOOOG010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44345_length_2788_cov_22.287962"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PK242_13005"
     CDS             <1..174
                     /locus_tag="PK242_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HOZ94982.1"
                     /translation="GDTPTLADCCLVPQIFNARRFDCDLSGLPRTMAVFDACMQLQAF
                     QNAQPSACPDAEA"
     gene            174..1001
                     /gene="pgeF"
                     /locus_tag="PK242_13010"
     CDS             174..1001
                     /gene="pgeF"
                     /locus_tag="PK242_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOZ94983.1"
                     /translation="MPAGLPAAAGPWLIPDWPLPPGVRALFTLRGQSAADGASDPPWA
                     HLNLGEHVGDATAAVAANRARLRAALGARPVFMHQVHGTHVLTLTPDCGDGTSADAAV
                     TDQPGLACTVMVADCLPVLLTDTRGRAVAAAHAGWRGLAAGVLEQTFKRLKALALDQQ
                     APPAIKMEADEWLAWLGPCIGPTAFEVGAEVHAAFLAGDAGAGSCFRPRPDGKFLADL
                     AALARRRLAALGVTRVYGNDGGPAWCTVGQPARYHSYRRDERALGGSGRMAACIWRV"
     gene            1192..>2788
                     /gene="phaC"
                     /locus_tag="PK242_13015"
     CDS             1192..>2788
                     /gene="phaC"
                     /locus_tag="PK242_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HOZ94984.1"
                     /translation="MSAGFPESMVGAAFGQGFAETWMKAFQHFQNMDLGKGAGEAPSI
                     DVPQLKFSPEALAKLQQDYVAEAAQLWNQTLQANPPLKDKRFAGEAWASNPLASFTAA
                     VYLLNARTLLAMADAVEGDAKARNRVRFAVEQWMAAAAPSNFLAFNPDAQRLAIDTHG
                     ASLAKGVANMLQDMKQGHVSMTDESVFQVGKNVATTEGAVVFENDLFQLIEYKPLTDK
                     VHEKPFLLVPPCINKFYILDLQPTNSFIRYAVSQGMRTFVVSWRNPDESLATATWDDY
                     IERAIIQAIRTVQDISGARQINALGFCVGGTMLTTGLAVLAARGEKPVSAATLLTTLI
                     DFTDTGILDVFIDEQMVRYREMQMGKGGLMKGQDMASTFSFLRPNDLVWNYVVGNYLK
                     GETPPPFDLLYWNSDSTNLPGPFYAWYLRNTYLENNLVKPGKATVCGEKIDLRKLDIP
                     FYLYGSREDHIVPIGGAYGSTQALPGKKRFVMGSSGHIAGVINPPAARKRSHWRRDDG
                     KFPKDVNDWIAGATEHPGSWWDDW"
BASE COUNT          467 a          993 c          916 g          412 t
ORIGIN      
        1 ggcgacacgc cgacgctggc cgactgctgc ctggtgccgc agatcttcaa cgcccggcgc
       61 ttcgactgcg acttgagcgg actgccgcgc accatggccg tgttcgacgc ctgcatgcag
      121 ctgcaggcgt tccagaacgc ccagccttcg gcctgccccg acgccgaggc ctgatgccgg
      181 ccggtctgcc cgccgccgcc ggcccctggc tgatccccga ctggccgctg ccgcccggcg
      241 tgcgggcgct gttcaccctg cgtgggcaaa gcgccgccga cggcgcgtcc gacccgccct
      301 gggcgcatct gaacctgggc gagcatgtgg gcgacgccac ggccgcggtg gcggccaacc
      361 gggcccgcct gcgcgccgcc ctgggcgcgc ggccggtgtt catgcaccag gtgcacggca
      421 cccacgtgct gaccctgaca cccgactgcg gcgatggcac cagcgccgat gcggccgtca
      481 ccgaccagcc gggcctggcc tgcaccgtca tggtggccga ctgcctgccg gtgctgctga
      541 ccgacacccg gggcagggca gtggccgcgg cgcacgccgg ttggcgcggg ctggcggccg
      601 gggtgctgga gcagaccttc aagcgtttga aggccctggc gcttgaccag caagcgccac
      661 cagctatcaa aatggaagcg gatgaatggc tggcctggct cggcccctgc atcggcccga
      721 ccgctttcga ggtgggcgcg gaggtgcacg ccgcctttct cgccggcgac gccggtgccg
      781 gctcctgctt ccgcccgcga ccggacggca aattcctggc cgatctggcg gcactggcgc
      841 ggcgccggct ggcggcactg ggcgtgacgc gggtgtacgg caacgacggc ggccccgcct
      901 ggtgcaccgt gggacagccg gcgcgctacc actcgtaccg gcgcgacgag cgcgccctgg
      961 gcggcagcgg ccgcatggcg gcctgcatct ggcgcgtgtg acgcggccct gcgtacccgt
     1021 ccggccaggc ctgcgctttg ccgaacacca tggcccgcag ccaatggccg gcgtatattg
     1081 gcggcgtgtc agggtggcgt tacgttcctg acgcatactg acggcaggct tggtcaggcg
     1141 ccccaccaca aaggagactt catgaacccg tccaacaacc cctggcaggc gatgtccgcc
     1201 ggattccccg agagcatggt aggcgcggca ttcgggcagg gttttgccga aacctggatg
     1261 aaggcgtttc agcatttcca gaacatggac ctgggcaagg gcgccggcga ggcgccgtcg
     1321 atcgacgtgc cgcagctcaa gttctcgccc gaggcgctgg ccaaactgca gcaggactac
     1381 gtggccgagg ccgcccagct gtggaaccag accctgcagg ccaacccgcc gctcaaggac
     1441 aagcgttttg ccggcgaggc ctgggcgtcc aacccgctgg cctcgttcac ggccgccgtg
     1501 tacctgctca acgcccgtac cctgctggcc atggccgatg cggtggaagg cgatgccaag
     1561 gccagaaacc gcgtgcgctt cgcggtcgag cagtggatgg ccgccgccgc gcccagcaac
     1621 ttcctggcct tcaaccccga cgcccagcgc ctggccatcg acacccatgg cgccagcctg
     1681 gccaagggtg tggccaacat gctgcaggac atgaagcagg gccatgtgtc gatgaccgac
     1741 gaaagcgtgt tccaggtcgg caagaacgtg gccaccaccg aaggcgcggt ggtgttcgag
     1801 aacgatctgt tccagctcat cgaatacaag ccgctgaccg acaaggtgca tgaaaagccc
     1861 ttccttctgg tgccgccctg catcaacaag ttctacatcc tcgatctgca gcccaccaac
     1921 tccttcatcc gctacgccgt cagccagggc atgcgcacct tcgtggtcag ctggcgcaac
     1981 cccgacgagt cgctggccac cgccacctgg gacgactaca tcgagcgcgc catcatccag
     2041 gccatccgca cggtgcagga catcagtggt gccaggcaga tcaacgcgct gggcttttgc
     2101 gtcggcggca ccatgctgac cacggggctg gccgtgctgg ccgcgcgcgg cgaaaagccg
     2161 gtctcggccg ccaccttgct gaccacgctg atcgacttca ccgacaccgg catcctggac
     2221 gtcttcatcg acgagcagat ggtgcgctac cgcgagatgc agatgggcaa gggcgggctg
     2281 atgaaggggc aggacatggc cagcaccttc agctttctgc ggcccaacga tctggtctgg
     2341 aactacgtgg tcggcaacta cctgaagggc gagacgccgc cgccgttcga cctgctgtac
     2401 tggaacagcg actccaccaa cctgcccggc ccgttctacg cctggtacct gcgcaacacc
     2461 tacctcgaaa acaacctggt caaacccggc aaggccaccg tctgcggcga gaagatcgac
     2521 ctgcgcaagc tcgacatacc gttttacctg tacggctcgc gtgaagacca tatcgtgccg
     2581 atcggcggcg cctacggcag cacccaggcg ctgccgggca agaagcgctt cgtcatgggc
     2641 tcgtcgggcc acatcgccgg cgtcatcaac ccgccggcgg ccagaaagcg cagccactgg
     2701 cggcgcgacg acggcaagtt cccgaaagac gtcaacgact ggatcgccgg cgccaccgag
     2761 caccccggca gctggtggga cgactggg
//
LOCUS       DAOOOG010000261         2771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_44711_length_2771_cov_22.542342, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000261 DAOOOG010000000
VERSION     DAOOOG010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44711_length_2771_cov_22.542342"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PK242_13020"
     CDS             complement(<1..378)
                     /locus_tag="PK242_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HOZ94985.1"
                     /translation="MTHTPLRARVAAALALLSLAACGGGDSADAGPPPVPPAWAWSLP
                     AGLAPPLVPADNPMNAAKVELGRHLFYDVRLSVNNTLSCAGCHAVNLAFADGRAVARG
                     ATGQNHPRNAPSLVNAAYQATLDW"
     gene            467..1441
                     /locus_tag="PK242_13025"
     CDS             467..1441
                     /locus_tag="PK242_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ94986.1"
                     /translation="MNMTARQDTDSFWARARRGLLVATGFALLSLAACGGNGDDEVAD
                     RGPTTIEVQAKLNGLYWDAQASRLYLTDDEANVNAIRIWDGDDKFSVAHQLPAMEANQ
                     RHSLGQLTRAADGKLYVTRFGFGSYGTVVAVPKSGQPYNLTGLAGDRRRIAITPTADG
                     KLIDGWFRGGGAPGPSGVISELTVAAGGQASEQDLITGLSKPAGVAVVGDQLFVSDQG
                     TGKVLAYSLAAVRAQPAKAEDGRVVAEFISADSIDLMTAGPDGTLFFGGGAGTLYAVA
                     PDGKVRTLASGWPGIRGVAYDAQNKRLFAAVTAPNAASPATIRIVPID"
     assembly_gap    1512..1611
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1716..2465
                     /locus_tag="PK242_13030"
     CDS             1716..2465
                     /locus_tag="PK242_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOZ94987.1"
                     /translation="MTKKTLSASSLGYGQADKRILILQLVGAHGSISQAARVAGVSYK
                     AAWQALHTLTNLAGEALVDSSVGGAGGGGARLTPAGERLLHAATRLAQARQAVLSQLG
                     AADDAIAAPRTSMRNHLRARVLELVGAGPREPMVRVRLALDGGAELAALITRESAELL
                     GLAPGLALLALTKATAVRVVPAASGAPRPGVLPGRVLRVFRGTHGDEVTLTLTGGQQL
                     VGFAERPNRLRAGSAALAQVDDRVVVLALPG"
     gene            complement(2497..>2771)
                     /locus_tag="PK242_13035"
     CDS             complement(2497..>2771)
                     /locus_tag="PK242_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HOZ94988.1"
                     /translation="ANLIGGYLVGLAVALLHHHPQLDPTWRLLLVTGFLGALTTFSSF
                     SAEVVDMALAQRWGLAAVTVLAHLSGSLLLTFAGIRTALVLIAARA"
BASE COUNT          407 a          915 c          983 g          366 t
ORIGIN      
        1 ccagtccagc gtggcctggt aggcggcgtt gaccaggctg ggtgcgttgc gcgggtggtt
       61 ctgcccggtg gcgccgcgcg ccacggcgcg gccgtcggcg aaggccaggt tgacggcgtg
      121 gcagccggcg caggacaacg tgttgttgac cgacaggcgc acgtcgtaga acaggtggcg
      181 ccccagctcc accttggctg cattcatcgg gttgtcggcc ggcaccaggg gcggcgccag
      241 gcctgccggc agtgaccagg cccaggctgg cggcaccggc ggcggtcctg catcggcgct
      301 gtccccgccg ccgcaggcgg caagcgacag cagggcaagt gcggcagcga cgcgagcgcg
      361 cagcggtgtg tgcgtcatgg tgggttcctc gtgggtatcg aacgtcgtta ttcaccgata
      421 gaatagcgca tcactccacc ttcaccacga tagaaaagga cgaaccatga acatgacggc
      481 acggcaggac acggactctt tctgggcgcg cgcgcggcgc gggctgctgg tggctaccgg
      541 gtttgccttg ctgtcgcttg ccgcctgcgg cggtaacggc gacgacgagg tggccgaccg
      601 tggcccgacc accatcgagg tccaggccaa gctcaacggc ctgtactggg atgcgcaggc
      661 atcgcgtctg tacctgaccg acgacgaggc aaacgtcaat gccatcagaa tctgggacgg
      721 cgacgacaag ttctccgtgg cgcaccagct gccggctatg gaggccaatc agcgtcactc
      781 gctgggccag ctcacgcgcg ctgcggacgg caagctgtac gtcacgcgct tcggcttcgg
      841 cagctacggc acggtggtcg ccgtgcccaa gtcgggccaa ccctacaacc tgacgggact
      901 ggccggcgac cgccggcgca tcgccatcac gcccacggcc gacggcaaac tgatcgacgg
      961 ctggttccgg ggtggcggcg cgcccggacc gagcggcgtc atcagcgagc tgaccgtggc
     1021 cgccggcggg caggccagcg agcaggacct gatcacgggc ctcagcaagc ctgccggggt
     1081 ggcggtggtg ggcgaccagc tgttcgtgtc cgatcagggc acgggcaagg tgctggccta
     1141 ttcgctggcc gccgtgcggg cgcagccggc gaaggccgag gatgggcgcg tcgttgccga
     1201 gttcatctcc gccgacagca tcgatctgat gacggccggg cccgatggca cgctgttctt
     1261 cggcggcggc gccggcacgc tgtacgcggt ggcgccggac ggcaaggtca ggacgctggc
     1321 cagcggctgg cccggcatcc gcggcgtggc ctacgacgcg cagaacaagc gcctgttcgc
     1381 ggccgtgacg gcgcccaacg ccgccagccc ggccaccatc cgcatcgtgc cgatcgattg
     1441 aacctcgaca cggcagttga ccgcttgtca tcatcgggca ggccgcatcc ctgcagggtt
     1501 tcgcggctgc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacggcgcg
     1621 ggcgggcgca tccaactgcc atttccaggt tgaagcgcct cgcggccgcc gtgcaagcgg
     1681 cagtgttcgc ctatgtctgc gagacaatgg gcgacatgac gaaaaagacc ttgtccgcct
     1741 ccagcctggg gtatggccag gccgacaagc gcatcctgat cctgcaactg gtgggtgcgc
     1801 acggctccat ctcgcaggcg gcgcgggtgg cgggcgtcag ctacaaggcg gcctggcagg
     1861 cgctgcacac gctgaccaac ctggcgggtg aggcgctggt ggacagcagc gtcggcggtg
     1921 ccggcggcgg cggcgcgcgc ctgacgcctg ccggcgaacg gctgttgcac gcggccaccc
     1981 gcctggcgca ggcgcgccaa gccgtgctgt cgcaactggg cgccgccgac gacgccatcg
     2041 ccgcgccacg caccagcatg cgcaaccatc tgcgcgcccg cgtgctcgaa ctggtgggcg
     2101 ccggcccgcg cgaacccatg gtgcgggtgc gcctggcgct cgatggtggc gccgagctgg
     2161 cggcgctcat cacgcgcgag agcgccgagc tgctgggcct ggcacccggg ctggcgctgt
     2221 tggcgctgac caaggccacc gccgtgcggg tggtgcccgc cgccagcggg gcgccgcggc
     2281 cgggggtgct gccggggcgc gtgctgcggg tgttccgcgg cacccacggc gacgaggtca
     2341 cgctgaccct gacgggtggc cagcaactgg tgggattcgc cgagcggccc aaccggctgc
     2401 gtgccggctc agccgccctg gcgcaggtcg acgaccgcgt ggtggtgctg gcgctgccgg
     2461 gctgaccgcg cgaaacactg cactagcgcc cgtccatcaa gcgcgagcag ctatcaaaac
     2521 cagagcggtc cggatgccgg cgaaggtcag cagcagcgag cccgacaggt gcgccagcac
     2581 ggtgaccgcg gccagccccc agcgttgcgc cagcgccatg tccaccactt cggcgctgaa
     2641 gctggaaaag gtggtcagcg cgcccagaaa gccggtcacc agcagcagcc gccaggtcgg
     2701 gtcgagctgc gggtgatggt gcagcagcgc caccgccagc ccgaccaggt agccgccgat
     2761 caggttggcc g
//
LOCUS       DAOOOG010000262         2765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_44847_length_2765_cov_26.685240, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000262 DAOOOG010000000
VERSION     DAOOOG010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44847_length_2765_cov_26.685240"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PK242_13040"
     CDS             <1..606
                     /locus_tag="PK242_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ94989.1"
                     /translation="AATRARLSHVLAAAPGIALVHTAASAREMLDWLAGNSVNVLLVD
                     LGLPDRSGLEVIRWCSTHQPATNVMVVTLFGDEAHMIGAFEAGARGYLLKDGTEDELA
                     RHVLSLHSGGSPMTPLIARQLLARLTSPARPPAPASQPISAREKDILQALSRGYTYDE
                     AARLLGIAPSTVQSHVKNIYSKLAVHSKTEAIFEARQLGLL"
     gene            649..2505
                     /locus_tag="PK242_13045"
     CDS             649..2505
                     /locus_tag="PK242_13045"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOZ94990.1"
                     /translation="MPATLPFWLRLLLAAALSLTGLAVAAAPQRVTQVMATLTPDGGP
                     PQTLEITLPHRWDDSFPGRGGRASYQFELPATPDPSPRALYLPRAGNQIEVWLNDRLI
                     HRVGRLGDQRTDAAKAPHWIPLPEVAHASGQPAARVQVRLSAQAARWGGLSAPWVGPR
                     DELYARYREHYRLRQYGGLIVCAVLGLMALMAAGFWWVQRDRLYLIFCLASAAGVLRI
                     ADRLIEHPPLPWPVWGLVTAWAMVALMVLMVYFALMLVHRQSLLDAPWFRLLVGLDLL
                     LTAAAYVLGLPQLWTLTLTSLVVPAILSWIAMAQAAWRQRSGQALAVLVAGSALIGTG
                     VRDWWNVRLYGDGAGTVSFMPHALLLFALLMGAVLVQRFARVAGERRSLLTTLEARVR
                     QREVELNQRHAELRQEYAHQAALQERQRLMRDIHDGVGVQLVGLLDLLDHEATPRELL
                     RQQLGTALDELRMAVDALQPTHGDLTTVLATLRYRLQPRLQAAGIAIDWQVDELPPIE
                     GLTPTTVLQLQRVLQEAFTNALRHARASRLWVRAWLDHGPPARLVLSVEDDGRGLPDD
                     GGATAPAGGQGLSSMQARAQAIGAELNVGPGAHGGTLVRLALPAGGAVGAPP"
BASE COUNT          358 a         1009 c          987 g          411 t
ORIGIN      
        1 gcggccacgc gcgcacgcct gagccatgtg ctggcggcgg cgccgggcat cgccctggtg
       61 cacacggcgg ccagcgcccg cgagatgctg gactggctgg ccggcaacag cgtcaacgtg
      121 ctgctggtgg acctggggct gcccgaccgc tcggggctgg aagtgatccg ctggtgcagc
      181 acccaccagc cggccaccaa cgtgatggtg gtcaccctgt tcggtgacga agcccacatg
      241 atcggcgcct tcgaggccgg cgcgcgcggc tacctgctca aggacggtac cgaggacgag
      301 ctggcgcgcc acgtgctcag cctgcattcg ggcggctcgc ccatgacgcc gctgatcgcg
      361 cgccagctgc tggcgcgcct gaccagcccg gcccgcccgc cggcgccggc gtcgcagccg
      421 atctcggcgc gcgagaagga catcctgcag gcgctgtcgc gcggctacac ctacgacgaa
      481 gccgcgcgcc tgctgggcat cgcgccctcg accgtgcagt cgcacgtgaa gaacatctac
      541 agcaagctgg ccgtgcattc caagaccgag gcgatcttcg aggcgcgcca gctgggcctg
      601 ctctgatagg ctggcgggca ccgaccccca gccagccgcg ccccgcccat gcccgccacc
      661 ctgcccttct ggttgcgtct gctgctggcc gccgcgctca gcctgaccgg gctggcggtg
      721 gccgcggcgc cccagcgcgt gacgcaggtc atggccacgc tgacaccgga cggcggcccg
      781 ccgcagacgc tggagatcac gctgccgcac cgctgggacg acagctttcc cggccggggc
      841 gggcgcgcca gctaccagtt cgagctgccc gccacgcccg accccagccc gcgcgcgctg
      901 tacctgccgc gcgcgggcaa tcagatcgag gtatggctga acgatcggct gattcatcgc
      961 gtcggccgct tgggcgacca gcgcaccgat gcggccaagg ccccgcactg gatacccctg
     1021 cccgaggtcg cacacgcctc cggccagccg gcggcgcgcg tgcaggtgcg gctgagcgcg
     1081 caggcggcgc gctggggcgg gctgtcggca ccctgggtgg ggccgcgcga cgagctgtac
     1141 gcgcgctacc gcgaacacta tcggctgcgt cagtacggtg gtctgatcgt ctgcgcggtg
     1201 ctgggcctga tggcgctgat ggcggccggc ttctggtggg tgcagcgcga ccgtctgtac
     1261 ctgattttct gcctggcgtc cgcggccggc gtgctgcgca tcgctgaccg gctgatcgaa
     1321 caccctcctc tgccctggcc cgtgtggggc ctggtgacgg cctgggccat ggtcgccctc
     1381 atggtcctga tggtctactt tgccctcatg ctggtacacc gacagagcct gttggacgcc
     1441 ccctggtttc gcctgctggt cggtctggat ctgctgctca ctgcggcggc gtatgtcttg
     1501 ggactacccc agctgtggac gttgacgctg acctcgctgg tggtgccggc catcctgtcc
     1561 tggatcgcca tggcgcaggc ggcctggcgc cagcgcagcg gccaggcact ggcggtgctc
     1621 gtggccggct cggcgctgat cggcacgggg gtccgtgact ggtggaacgt gcgtctgtat
     1681 ggcgacggcg ccggcacggt ctcgttcatg ccgcatgcgc tgctgctgtt cgcgctgctg
     1741 atgggcgccg tgctggtgca gcgctttgcg cgcgtggcgg gcgaacgacg ctcgctgctg
     1801 accacgctgg aggcgcgcgt gcgccagcgc gaagtcgaac tgaaccagcg ccacgccgaa
     1861 ctgcgccagg agtacgccca ccaggcggcg ctgcaggagc gtcagcgcct gatgcgcgac
     1921 atccatgacg gcgtcggcgt gcaactggtg ggcctgctcg acctgctgga ccacgaggcg
     1981 acgccgcgcg agctgctgcg ccagcagctg ggcacggcgc tggacgagct gcgcatggcg
     2041 gtggacgcgc tgcaacccac gcacggtgac ctgacgacgg tgctggccac gctgcgctac
     2101 cgcctgcaac cgcgcctgca ggcggccggc atcgccatcg actggcaggt ggacgagctg
     2161 ccacccatcg aaggcctgac gccgaccacg gtgctgcaac tgcagcgcgt gctgcaggag
     2221 gccttcacca acgccctgcg ccacgcccgg gccagccgcc tgtgggtgcg cgcctggctg
     2281 gaccacggcc cgcccgcgcg cctggtgctg agcgtggagg acgacggccg cggcctgccc
     2341 gacgatggcg gcgccaccgc gccggctggc ggccaggggc tgagcagcat gcaggcgcgc
     2401 gcccaggcca tcggcgccga gctgaacgtc ggccccggcg cgcatggcgg caccctggtg
     2461 cgcctggcgc tgccggcggg cggcgcggtg ggcgcgccgc cctgacgctg gccgccccgc
     2521 ccgccccggg tgcggttttg gtgctcgaac aggggttttg cgcccggtga accagcgcca
     2581 gcagctattg aatcgatagt tctttttgta caagtccagg cggcgcaggg cgtcggcggg
     2641 cgccgcaacg cccttacact gcggctatcc gcacgccctc tcaaccatgg ttccccacct
     2701 cgtcaccgcc ctgaccggcc ccatcaacga gctggagcag cgcatcctcg acgccacgcc
     2761 ggtga
//
LOCUS       DAOOOG010000263         2759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_44971_length_2759_cov_27.894601, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000263 DAOOOG010000000
VERSION     DAOOOG010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44971_length_2759_cov_27.894601"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /gene="rpsL"
                     /locus_tag="PK242_13050"
     CDS             complement(<1..299)
                     /gene="rpsL"
                     /locus_tag="PK242_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOZ94991.1"
                     /translation="MPTISQLVRHGRTVETTKSKSPAMQNSPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRG"
     gene            complement(635..1795)
                     /locus_tag="PK242_13055"
     CDS             complement(635..1795)
                     /locus_tag="PK242_13055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOZ94992.1"
                     /translation="MIPLLSRTLVATLLAVTTLVGHAQAPQAPEVAAKAYLLMDVSAG
                     QVLAAKDIDAPVEPASLTKLMTAYLVFDALKARKISLNQTLPVSERAWKMPGSRMFID
                     PKMQVPVEDLIKGMIVQSGNDATMALAEGVGGTAERFVQLMNEQAKAMGLAGTQYRNP
                     EGLTEPGHHTTARDLATLSTRLLRDFPEYLHYYTIRKYRYPGTPASNDTNRNTLLFRD
                     PTVDGLKTGHTSAAGYCLVATARRDFPNLPGRRLLSVVLGASSETARANESQKLLNWG
                     YTAYEAVKLFDGGQPVASPKVWKGKSAELKLGRGEPIVVAVPSGSAGQIKTEIARPEP
                     LIAPFRQGQAVATLKVRQGEQTLAEVPLVALEAVEEAGFLGRAWDAMRLWIK"
     gene            complement(1866..2501)
                     /locus_tag="PK242_13060"
     CDS             complement(1866..2501)
                     /locus_tag="PK242_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ94993.1"
                     /translation="MNAQTESLTLRGPAGAIEALLDRPAAGGAARGTAVIAHPHPQFG
                     GTMSNKVVQTLARACVQCGWRAVRFNFRGVGASAGQYDHGQGEQDDLLAVVAQQAAAD
                     QALLLSGFSFGAFVASQAIARLHPQRALAGAVLVGTAASRFDVAPLPPALHAQTLVVH
                     GEHDETVPLTSVMDWARPQLLPVTVVPGGGHFFHGQLPLLKGLVERHLRAL"
     gene            complement(2587..>2759)
                     /locus_tag="PK242_13065"
     CDS             complement(2587..>2759)
                     /locus_tag="PK242_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOZ94994.1"
                     /translation="KAGCLDRCAAGPVAVVYPEGVWYSYVDVSDIDEIVDAHLKRGQI
                     VERLLTPPHLGR"
BASE COUNT          429 a          929 c          970 g          431 t
ORIGIN      
        1 ccgcgcacga tgtggtagcg cacaccgggc aggtccttga cccggccgcc gcgcaccagc
       61 accacgctgt gctcttgcag gttgtggcct tcgccaccga tgtaggagat gacctcgaag
      121 ccgttggtca ggcgcacctt ggcgaccttg cgcagcgccg agttcggctt cttgggcgtg
      181 gtggtgtaca cgcgcgtgca cacgccgcgg cgttgcggcg agttctgcat cgccgggctc
      241 ttggacttgg tcgtctcgac cgtccgcccg tgacgcacca gttgactgat ggttggcatg
      301 aaattccctt gaaacgtgaa aacgcgcgtg cagcgcgcgc cgaaaaaccc cgcaacgcca
      361 gcaaggcgag cgccctcacc caagatggcg cgcgcggcaa aaatacggga aaagccgcag
      421 attatagccc gccggcgcgg gcgctacaag ccgctgccgc ctgcgcgccg gactcggagt
      481 gcgggcacgg gttgcgggcg ccagagagtc gcgccccaac acgggcgatc aaggcaccag
      541 gcacagccgc agcctgagcg gcgcgagcat gggccgcgcc gcgcgggtgc acctgggcga
      601 tcgaggcggg caggcgggat cgggtcaccc cgtctcactt gatccacagt cgcatggcgt
      661 cccaggcccg acccaggaag ccggcctcct cgaccgcttc cagcgccacc agcggcactt
      721 cggccagggt ctgctcgccc tggcgcacct tcagcgtcgc caccgcctgg ccctggcgga
      781 acggggcgat cagcggctcg gggcgggcga tttcggtctt gatctggccg gcgctgccgc
      841 tgggcacggc gaccacgatg ggctcgccgc gacccagctt cagctcggcc gatttgccct
      901 tccacacctt ggggctggcc accggctggc caccgtcgaa cagcttgacc gcctcgtagg
      961 cggtgtaacc ccagttgagc agcttctgcg actcgttggc gcgcgccgtc tcgctgctgg
     1021 cgcccagcac caccgacagc aggcgccggc ccggcaggtt gggaaagtcg cgccgcgccg
     1081 tggccaccag gcagtagccg gcggcgctgg tgtggccggt cttcaggcca tcgacggtgg
     1141 ggtcgcgaaa cagcagggtg ttgcggttgg tgtcgttgct ggcgggcgtg ccggggtagc
     1201 ggtacttgcg aatggtgtag tagtgcaggt actccggaaa gtcgcgcagc agccgggtgg
     1261 acagggtggc cagatcgcgc gcggtggtgt ggtggccggg ctcggtcagg ccttcggggt
     1321 tgcggtactg ggtgccggcc aggcccatgg ccttggcctg ctcgttcatc agctgcacga
     1381 agcgctcggc ggtgccgccg acgccctcgg ccagcgccat ggtggcgtcg ttgccggact
     1441 gcacgatcat gcccttgatc aggtcttcca ccggcacctg catcttgggg tcgatgaaca
     1501 tgcgcgagcc gggcatcttc caggcgcgct cggacaccgg cagggtctgg ttcaggctga
     1561 tttttctggc cttcagggcg tcgaacacca ggtaggccgt catcagcttg gtgagcgagg
     1621 ccggctccac gggggcgtcg atgtccttgg cggccagcac ctggccggcc gaaacgtcca
     1681 tcagcaaata ggccttggcg gccacttcgg gcgcctgcgg cgcctgggcg tgacccacca
     1741 gggtggtgac cgccagcagc gtggcaacca gggtacggga cagcaaaggg atcatcatga
     1801 actatgaaaa tgatagctta tggcgcttgc cagtcgggcg ccagagcctg aaaaatcttg
     1861 gaacatcaaa gggcgcgcag gtggcgttcg accaggccct tgagcagcgg caattgtccg
     1921 tgaaagaagt gaccaccccc cggcaccacg gtgaccggca gcagttgcgg ccgcgcccag
     1981 tccatcaccg aggtcagcgg cacggtctcg tcgtgctcgc catggaccac cagggtctgg
     2041 gcgtgcagcg ccggcggcag cggcgccacg tcgaagcgcg aggccgccgt gcccaccagc
     2101 accgcaccgg ccagcgcccg ctgcgggtgc agtcgggcga tggcttgcga ggccacgaag
     2161 gcgccgaacg aaaaaccgga cagcagcagg gcctggtcgg ccgccgcctg ctgcgccacc
     2221 acggccagca ggtcgtcctg ctcgccctgg ccgtggtcgt actgcccggc actggcacca
     2281 acgccgcgga agttgaagcg caccgcacgc cagccgcatt gcacgcaggc gcgcgccagg
     2341 gtctgcacca ccttgttgct catggtgccg ccaaactgcg ggtgcgggtg ggcgatcacg
     2401 gcggtgccgc gcgctgcgcc gcccgccgcc ggacgatcga gcagggcttc gatcgcaccg
     2461 gctggcccgc gcagcgtcag ggattcggtc tgggcattca ttcagctatc taaattgtag
     2521 ctgctcgcgc ttgctgcgcc ggcgccggaa ggtgaaaaag cttgtaaccc gacgcccggt
     2581 cgggcgtcag cgtccgaggt gtggcggcgt cagcaggcgc tcgacgatct ggccgcgctt
     2641 gaggtgcgcg tcgacgattt cgtcgatgtc gctgacgtcc acgtagctgt accagacgcc
     2701 ctccgggtac accaccgcca ccgggccggc ggcgcagcgg tccaggcagc cggccttgt
//
LOCUS       DAOOOG010000264         2756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_45023_length_2756_cov_22.401333, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000264 DAOOOG010000000
VERSION     DAOOOG010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45023_length_2756_cov_22.401333"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1643
                     /locus_tag="PK242_13070"
     CDS             336..1643
                     /locus_tag="PK242_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOZ94995.1"
                     /translation="MNQAPDRAPAPPLAERLRPRTLAEVVGQPHLLGPGMPLRIAFES
                     GRPHSCILWGPPGTGKTTIARLMADAFDAQFIAISAVLGGVKDIRDAVERATQAEQGL
                     APRRTIVFVDEVHRFNKSQQDAFLPHVESGLFTFIGATTENPSFEVNSALLSRATVYV
                     LQSLTADDLKEIVARAQAQQALPAIDSVALERLLGYADGDARRLLNTLETVAVTAAAE
                     GVAQIDEAWLLKVLGQQLRRYDKGGEQFYDTISALHKSVRGSDPDAALYWLVRMLDGG
                     ADPRYLARRMLRMASEDIGLADPRALRLALDAAEVYERLGSPEGELALAECVVYLAVA
                     PKSNAVYKAYNAARAWVRQDVTRPVPLHLRNAPTRLMKQLDYGKGYRYAHDEEGGFAA
                     GERYLPEGMPDQRFYDPVPRGLEIRIGEKLAELRRRNREAAGG"
     gene            1880..>2756
                     /locus_tag="PK242_13075"
     CDS             1880..>2756
                     /locus_tag="PK242_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ94996.1"
                     /translation="MDILLQQIINGLVLGSMYALIALGYTMVYGIIQLINFAHGEVLM
                     VGALVAWTVIGVLQEAVPGMPTWLILLAATVAGCVVAAVLNIVIEKVAYRPLRNAPRL
                     APLITAIGMSILLQALAMVIWKPNVKSFPSMLPTEPYFIGEAVITPTQIMILVVTVMS
                     LAVLMYIVHYTRIGRAMRATAENPRVAALMGVKPDAVISATFAIGAVLAAIAGVMYAS
                     NYGTAQHFMGFLPGLKAFTAAVFGGIGNLTGAMVGGVLLGLIEAIGSGYLGALTGGLL
                     GSHYADIFAFIVLIIV"
BASE COUNT          441 a          920 c          938 g          457 t
ORIGIN      
        1 ttcatcaggg tttgctgcca ggcaaaggca gcgtcaataa ccgtttgtag tcacaccgtc
       61 cgcaccgttt gccaagaggc attgacggac tgcacgcaca aggtgggagc ctgatcgcag
      121 gtcaatgagc aaaccgatcc tggattgatc gctgtgcgat caacctaggc aattctgctg
      181 gtgggcaccg tacgactgcg cccgggccga cggcaatggg cgcaccatgc agcgcagtac
      241 gatcggcacc tgactagccc tggcgctgcc aagtcgcagc gggttgatcc gtcccagcgg
      301 ccaggcgagt ccttgctctt tgggcctctc tacccgtgaa ccaagctccc gaccgcgcac
      361 ctgccccacc cctggccgag cgcctgcgcc cgcgcacgct ggccgaggtg gtgggccagc
      421 cgcacctgct ggggccgggc atgccgctgc gcatcgcgtt cgagtcgggg cggccgcaca
      481 gttgcatcct gtgggggccg ccgggcacgg gcaagacgac gattgcgcgg ctgatggccg
      541 atgcgttcga cgcgcagttc atcgccatca gcgcggtgct gggcggcgtc aaggacattc
      601 gcgacgcggt cgagcgcgcc acgcaggcgg agcagggcct ggcgccgcgg cgcaccatcg
      661 tgttcgtcga cgaggtgcac cgcttcaaca agagccagca ggacgccttt ctgccgcacg
      721 tcgaaagcgg gctgttcacc ttcatcggcg ccaccaccga aaacccttcg ttcgaggtca
      781 actccgccct gctgtcgcgc gccacggtgt acgtgctgca atcgctgacg gccgacgatt
      841 tgaaggaaat tgtggctcgg gcgcaggcgc agcaagcgct gccagctatt gattcggtag
      901 cgttggagcg cctgctcggc tatgccgacg gcgacgcgcg gcgcctgctg aacacgctgg
      961 agacggtggc cgtgaccgcc gccgccgagg gtgtggcgca gatcgacgag gcctggctgc
     1021 tgaaggtgct cggccagcaa ctgcgccgct acgacaaggg cggcgagcag ttctacgaca
     1081 ccatcagcgc gctgcacaag tcggtgcgtg gctcggatcc cgacgccgcc ctgtactggc
     1141 tggtgcgcat gctcgacggc ggggccgacc cgcgctacct ggcgcgccgc atgctgcgca
     1201 tggccagcga agacatcggc ctggccgacc cgcgcgcgct gcgcctggcg ctggatgcgg
     1261 ccgaggtgta cgagcgcctg ggcagccccg agggcgagct ggcgctggcc gagtgcgtgg
     1321 tctacctggc ggtggctccc aagagcaacg cggtatacaa ggcttacaac gccgcccgcg
     1381 cctgggtgcg gcaggacgtg acacggccgg tgccgttgca tctgcgcaac gcgccgaccc
     1441 ggctgatgaa acagctcgac tacggcaagg gttaccgcta cgcgcacgac gaagagggcg
     1501 gctttgccgc cggcgagcgc tacctgcccg agggcatgcc cgatcagcgc ttttacgacc
     1561 cggtgccgcg cgggctggag atccgcatcg gcgaaaagct ggccgagctg cgccgacgca
     1621 accgcgaggc cgctggcggc tgaggcgtac gcgctggctg gggcgcgcgc ggccgccgtc
     1681 tacaagggaa atcccgggtc gggcagatcg tccgcaacac ccggcgtgcc gataaaatcg
     1741 cgctcgcttt ttgaacccgc aacggagtct ccggcgggtt ttttgttagc cgaccggcaa
     1801 gcccacaagg cgactccagg cggccgactg aaccacgccg cgccggagac aacggtgcgg
     1861 acccttgagg aagtctcgca tggacattct gcttcagcag atcatcaacg gcctggtgct
     1921 gggcagcatg tacgccctca tcgccctggg ctacaccatg gtttacggca tcatccagct
     1981 catcaacttc gctcacggcg aggtgttgat ggtgggcgca ctggtggcct ggaccgtcat
     2041 cggcgtgctg caggaggcgg tgcccggcat gcccacctgg ctgatcttgc tggcggccac
     2101 cgtggccggc tgtgtggtgg cggcggtgct caacatcgtg atcgagaagg tcgcttaccg
     2161 gccgctgcgc aacgccccac gcctggcgcc gctgatcacc gccatcggca tgtcgatcct
     2221 gctgcaggcg ctggccatgg tgatctggaa acccaacgtc aagtccttcc cgtccatgct
     2281 gccgaccgag ccgtacttca tcggcgaggc ggtgatcacg ccgacgcaga tcatgatcct
     2341 ggtggtcacg gtcatgtcgc tggcggtgct gatgtacatc gtgcactaca cgcgcatcgg
     2401 ccgcgccatg cgcgccaccg ccgaaaaccc gcgcgtggcg gcgctgatgg gtgtcaagcc
     2461 cgacgcggtg atttcggcca ccttcgccat cggtgcggtg ctggcggcca ttgccggggt
     2521 gatgtacgcc tccaactacg gcacggcaca gcacttcatg ggcttcctgc cggggctgaa
     2581 ggccttcacg gcggcggtgt tcggcggcat cggcaacctg accggcgcca tggtgggcgg
     2641 cgtgctgctg gggctgatcg aggccatcgg ctcgggctac ctgggcgcgc tcaccggcgg
     2701 cctgctgggc agccactacg ccgacatctt tgccttcatc gtgctgatca tcgtgc
//
LOCUS       DAOOOG010000265         2734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_45515_length_2734_cov_28.328108, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000265 DAOOOG010000000
VERSION     DAOOOG010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45515_length_2734_cov_28.328108"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PK242_13080"
     CDS             <1..293
                     /locus_tag="PK242_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOZ94997.1"
                     /translation="LQRYKELRDIIAILGMDELSPEDKLAVARARKIQRFLSQPFHVA
                     EVFTGSPGKYVPLKDTIHGFKMIVNGECDHLPEQAFYMVGTIEEAMEKAKKV"
     gene            387..806
                     /locus_tag="PK242_13085"
     CDS             387..806
                     /locus_tag="PK242_13085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOZ94998.1"
                     /translation="MANTIHVDVVSAEESIFSGEATFVALPGEAGELGIYPRHTPLIT
                     RIKPGSVRIQLPGGGEEFVFVAGGILEVQPDVITVLSDTAIRGKDLDEAKASEARKQA
                     EEALKNAKSEIDMARATSELAVLAAQIAALRKFRQKR"
     gene            921..1517
                     /locus_tag="PK242_13090"
     CDS             921..1517
                     /locus_tag="PK242_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOZ94999.1"
                     /translation="MMPDQERSDQSASLRAAALLVTEGALVELSAADAREVVSYMRPQ
                     RVGAGQIVIRAGETRRNDFMALVLHGEVTVENAPAASHEGMVVSVLGPGSLIGEMGLV
                     DGGPRSATCTANSDLVLAVLTREAVLHMMDARPAVAARLMLAIAKRIADHLRDTNRKL
                     MTLARVSKALQQELDAVHAVNKRLLDQVPATPVAKARG"
     gene            complement(1581..2120)
                     /locus_tag="PK242_13095"
     CDS             complement(1581..2120)
                     /locus_tag="PK242_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOZ95000.1"
                     /translation="MWTTLTRLGRHLWLDRSHTARAIPAATAERLRQRIAASEQRHSG
                     EVRICVEASLPASYLWRHLRQRLPIRQIVRQRALMMFSKLRVWDTEQNNGVLIYLLLA
                     ERAIELVADRGVMRVVEPTAWHEAVAHLGAAWHDGRFEEGLTQALEEVSAMLVAHFPN
                     SGERQGPAPHQLPDEPVLR"
     gene            complement(2124..>2734)
                     /locus_tag="PK242_13100"
     CDS             complement(2124..>2734)
                     /locus_tag="PK242_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOZ95001.1"
                     /translation="AVQDRRMRIEVAKTLEGAVPDIAARQIIDEAMAPHFRRGDYAGG
                     LDAAVEQLGARIRGEPLPPVRTTGTGTLQDELDFSLFEMLVLLFIIVPIIGAVARGVL
                     GRKLGALATGAGVGWLAFVVTASWLLALVAAALSLFIALLGGVSGALPSPRSRGGPWG
                     GGFGGGGLGGGGFGGGSFGSGGGGFSSGGGGDFGGGGASGSW"
BASE COUNT          461 a          973 c          920 g          380 t
ORIGIN      
        1 cgctgcagcg ctacaaggaa ctgcgcgaca tcatcgccat tctgggcatg gacgagctct
       61 cgcccgagga caaactggcc gtggcacgcg cgcgcaagat ccagcgcttc ctgtcgcagc
      121 cgttccacgt ggccgaggtg ttcaccggct cgcccggcaa gtacgtgccg ctgaaggaca
      181 ccatccacgg cttcaagatg atcgtcaatg gcgagtgcga ccacctgccc gaacaggcct
      241 tctacatggt cggcaccatc gaagaagcga tggaaaaggc caagaaagtc tgaacttcgg
      301 gttcggcgtc cggcgttacg tgctgagcgc ggcggtccga aggatcggcc catcgttcag
      361 cgcgcaacgc ccaaccagga gaaagcatgg ccaataccat tcacgtggac gtggtgagcg
      421 ccgaggagtc gatcttctcg ggcgaggcca cgttcgtggc gctgcccggc gaagccggcg
      481 aactgggcat ctacccgcgc cacacgccgc tgatcacgcg catcaagccg ggctcggtgc
      541 gcattcagct gcccggcggc ggcgaggaat tcgtctttgt cgcgggcggc attctggaag
      601 tgcagcccga cgtcatcacg gtgctgtccg acaccgccat tcgcggcaag gacctggacg
      661 aagccaaggc cagcgaggca cgcaagcagg ccgaagaagc gctgaagaac gccaagagcg
      721 agatcgacat ggcgcgcgcc acgtccgagc tggcggtgct ggcggcgcag atcgccgcgc
      781 tgcgcaagtt ccggcaaaag cgctgaacgg cagcttgatc gccgccaacc ggcatttgtc
      841 cacaagcccg ccttcgagcg ggctttttgt tgcggcgtgc ggcgccctcg cgtgcgtgtc
      901 gtccctctaa aatcctcccc atgatgcctg accaggaacg ctccgaccag agcgcctcgc
      961 tgcgcgccgc ggccttgctc gtcaccgagg gggcgctggt cgagctcagc gccgccgatg
     1021 cgcgcgaggt ggtcagctac atgcgaccgc aacgcgtggg tgcggggcag atcgtcatcc
     1081 gcgccggcga gacccggcgc aacgacttca tggcgctggt gctccacggc gaggtgacgg
     1141 tcgagaacgc ccccgccgca agccacgaag gcatggtggt gtcggtgctg ggcccgggca
     1201 gcctgattgg cgaaatgggt ctggtggacg gcgggccgcg ctcggccacc tgcaccgcca
     1261 actcggatct ggtgctggcg gtgctgacgc gcgaagccgt gctgcacatg atggacgcgc
     1321 gccccgcggt ggcggcacgc ctgatgctgg cgattgccaa gcgcattgcc gaccatcttc
     1381 gcgacaccaa ccgcaagctg atgaccctgg cgcgggtgag caaggcgctg cagcaggagc
     1441 tcgacgccgt gcacgcggtc aacaagcgcc tgctggatca ggtgccggcg acgccggtgg
     1501 ccaaggcccg cggctgagcg ccggcgactg cgcctgttga ttcggccttt ggatgccgac
     1561 aggcttacga agcagtaggc tcagcgcagc accggctcgt ccggcagctg atgcggcgcc
     1621 gggccctggc gctcgcccga gttggggaag tgcgccacca gcatggccga cacttcttcc
     1681 agcgcctgcg tcaacccctc ttcgaagcgg ccgtcgtgcc acgccgcgcc caggtgggcc
     1741 accgcctcgt gccaggccgt cggctccacc acccgcatca cgccgcggtc ggccaccagc
     1801 tcgatcgccc gctcggccag cagcaggtag atcagcacgc cattgttctg ctcggtgtcc
     1861 cacacgcgca gcttggaaaa catcatcagc gcgcgctggc gcacgatctg cctgattggc
     1921 aggcgctggc gcaggtgacg ccacaggtag ctggccggca ggctggcctc gacgcagatg
     1981 cgcacctcac cgctgtggcg ttgctcgctg gcagcgatgc gctggcgcag cctttcggcc
     2041 gtggcggccg ggatggcgcg cgcggtgtgg gaccgatcca gccagaggtg gcgccccagg
     2101 cgcgtcaagg tggtccacat ggtctaccag ctcccactgg cgccgccgcc accaaaatcg
     2161 ccgccgccgc ctgaactgaa gcccccgccg ccggagccga aactcccgcc gccgaagccg
     2221 ccgcccccaa gccctccgcc gccgaagccg ccgccccagg ggccaccgcg cgagcgcggc
     2281 gaaggcagcg caccgctcac accgcccagc agcgcgatga acagcgagag cgccgcggcc
     2341 accagcgcca gcagccagct ggcggtgacc acgaaggcca gccagcccac cccggcgccg
     2401 gtggccaggg cgcccagctt gcgaccgagc acgccgcgcg ccacggcgcc gatgatcggc
     2461 acgataatga acagcagcac cagcatctcg aacaggctga agtccagctc gtcctgcagc
     2521 gtgccggtgc ccgtggtgcg cacgggcggc agcggttcac cgcggatgcg ggcgcccagt
     2581 tgctcgacgg cggcgtccag cccgccggca tagtcgccgc gccgaaaatg gggcgccatg
     2641 gcctcgtcga tgatctgacg cgcggcgatg tcgggcaccg ccccttccag cgtcttggcg
     2701 acttcgatgc gcatgcgccg gtcctgcacc gcca
//
LOCUS       DAOOOG010000266         2729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_45634_length_2729_cov_21.261780, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000266 DAOOOG010000000
VERSION     DAOOOG010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45634_length_2729_cov_21.261780"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PK242_13105"
     CDS             <1..696
                     /locus_tag="PK242_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOZ95002.1"
                     /translation="DYGPNGLQVEGRPRIRRIVSGVTASLALIDAAIAADADAIFVHH
                     GLFWRGQDGTVTGWLKQRLQRLLAHDINLLAYHLPLDAHPTLGNNAQLAQRLGLRASG
                     RFGEQELGFIGEPADERGFDHAEALARHLQQVLNRPVTQVGQASTAIKKIAWCSGGAQ
                     GYFEAAIAAGAQAFITGEISEPQAHLARECGVVFCACGHHATERYGAPAMAAHVAARL
                     GIAHQFIEIDNPA"
     gene            781..1806
                     /gene="pdxA"
                     /locus_tag="PK242_13110"
     CDS             781..1806
                     /gene="pdxA"
                     /locus_tag="PK242_13110"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOZ95003.1"
                     /translation="MIEPLAITLGDAAGIGPEIIAKAFRDAPDALRGCFVAGDLATLR
                     RAAAVVGGGGVELPVAALDRPADALDAPPRCVPVLQVVEPQQPVPFGRISAQAGRAAG
                     DCVAWAARAALRGEVAALVTAPLHKEALAAAGAPYDRYPGHTELLQALAAEHLGVPVT
                     DLPVRMMLANDELRTVLVSIHLSLRAAIDQVTSGNIAETLRITHESVAAALRRPPRIA
                     VAGLNPHAGEGGLFGREDIEVIAPAVAAARARGIDARGPFAPDTVFMRARGFAEFDVV
                     VAMYHDQGLIPVKYLGVEQGVNVTLGLPLVRTSPDHGTAFDLAGSGRADPASLIAAVR
                     MARQLAP"
     gene            complement(1877..2299)
                     /gene="mscL"
                     /locus_tag="PK242_13115"
     CDS             complement(1877..2299)
                     /gene="mscL"
                     /locus_tag="PK242_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOZ95004.1"
                     /translation="MGMMQEFREFAVKGNAIDLAVGVIIGGAFGKIVDSIVGDLVMPV
                     VGKLLGGLDFSNHYIALAGQAAGLSLEEAKKAGAVFAYGNFITILVNFILLAFIIFMM
                     VKQINRLKKEEPATAEPAPAEPPEQVQLLREIRDSLKR"
     gene            2455..>2729
                     /locus_tag="PK242_13120"
     CDS             2455..>2729
                     /locus_tag="PK242_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit N-terminal domain-containing protein"
                     /protein_id="HOZ95005.1"
                     /translation="MSDAPPSNFTPAPMDSSKRTWLITSSCVGAAGAAALATPFVTSF
                     APSERAKAAGAAVEVDIAGLKPGEKLTVEWRGKPVWIMRRTPEQMAAL"
BASE COUNT          437 a          940 c          940 g          412 t
ORIGIN      
        1 gactacggac ccaacggcct gcaggtggaa gggcggccgc gcatccgcag gatcgtcagc
       61 ggcgtcaccg ccagtctggc gctgatcgac gccgccatcg ccgccgatgc cgacgccatc
      121 ttcgtgcacc acggcctgtt ctggcgcggc caggacggca ccgtcaccgg ctggctgaag
      181 cagcgcctgc agcgcctgct ggcccacgac atcaatctgc tggcctacca cctgccgctc
      241 gacgcccacc ccacgctggg caacaacgcc cagctggccc agcgcctggg cctgcgggcc
      301 agcgggcgct ttggcgagca ggagctgggc ttcatcggcg agccggccga tgaacgcggg
      361 tttgaccatg ccgaggccct ggcccgccac ctgcaacagg tgctgaatcg gccagtcacg
      421 caagtggggc aagcgtcgac agctatcaaa aaaatagctt ggtgcagcgg tggggcgcag
      481 ggttatttcg aggccgccat cgccgccggg gcgcaggcct tcatcaccgg cgagatatcc
      541 gaaccgcagg cgcatctggc gcgcgagtgc ggcgtggtgt tctgcgcctg tggccaccac
      601 gccaccgagc gctacggcgc gccggccatg gcggcccatg tggcggcgcg actggggatc
      661 gcgcaccagt tcatcgaaat cgacaaccct gcatgaacgc gcaccaacgg gggatgctga
      721 gagctctcaa aaacatagct ttcggcgcag tctgaaaaag ggctgaaggc gaatttgacc
      781 atgattgaac cgctggccat caccctgggc gacgccgccg gcatcgggcc ggagatcatc
      841 gccaaggcgt tccgcgacgc gcccgacgca ctgcgcggct gcttcgtggc cggcgacctg
      901 gcgacgctgc ggcgcgccgc ggcggtggtg ggcggcggcg gcgtcgaact gcccgtggcc
      961 gcgctcgatc ggccggccga tgcgctggac gcgccgccgc gctgcgtgcc ggtgctgcag
     1021 gtggtcgagc cgcagcagcc ggtgccgttc ggccgcatca gcgcgcaggc cggccgcgcc
     1081 gccggcgact gcgtggcgtg ggcggcgcgg gcggcgctgc gcggggaggt ggcggcgctg
     1141 gtcactgcgc cgctgcacaa ggaggcgctg gccgcggccg gcgcgcccta cgaccgctac
     1201 cccggccaca ccgagctgct gcaggcgctg gcggccgagc acctgggggt gccggtgacc
     1261 gatctgccgg tgcgcatgat gctggccaac gacgagttgc gcaccgtgct ggtcagcatc
     1321 cacctgtcgc tgcgtgcggc gatcgaccag gtaaccagcg gcaacattgc cgaaaccctg
     1381 cgcatcacgc acgaatccgt ggcggcggcg ctacggcggc cgccgcgcat cgcggtcgcc
     1441 ggcctgaatc cgcacgccgg tgaaggcggc ctgttcggtc gggaggacat cgaggtcatc
     1501 gcccccgccg tggccgcggc gcgcgcccgt ggcatcgacg cccgtggccc gttcgcgccc
     1561 gacacggtgt tcatgcgcgc gcgcgggttt gccgaattcg acgtggtggt ggcgatgtac
     1621 cacgaccagg gcctgatccc ggtgaaatac ctgggtgtgg agcagggcgt caacgtcacc
     1681 ctgggcctgc cgctggtgcg caccagcccc gaccatggca cggcgttcga cctcgccggc
     1741 agcggccggg ccgaccccgc cagcctgatc gcggcggtgc gcatggcccg tcagctggca
     1801 ccatgaaaac gcccgcagca tcgtgcgggg cgtgcgccga aagctacaaa taaagtagca
     1861 ttcggagcga tcggcgtcag cgcttcaggc tgtcgcggat ctcgcgcagc agctgcacct
     1921 gttccggcgg ttcggccggg gccggctcgg cggtcgccgg ctcttccttc ttcaaccggt
     1981 tgatctgctt gaccatcatg aagatgatga aggccagcag gatgaagttc accaggatgg
     2041 tgatgaagtt gccgtaggcg aacacggcgc ccgccttctt ggcctcttcc aggctcaggc
     2101 ccgcagcctg tccggccagc gcaatgtagt ggttggaaaa atccagcccg ccgagcaatt
     2161 taccgacgac gggcatcacc agatcgccga cgattgagtc gacgatcttg ccgaatgcgc
     2221 cgccgatgat cacgcccacc gccagatcga tggcgttgcc cttgacggcg aattctctga
     2281 actcctgcat catgcccatg tgcattctct ttcgtgtggt tgacggtcgc gacagtatgg
     2341 ccggggcctg atccgtgtca atcgcaggcc ggttgacggc cggtgcgcgc ttgacgctcc
     2401 gctacaatcg accgttgatt ccaagataag aaaccttacc cgaggaaccc caccatgagt
     2461 gacgcccctc ccagtaactt cacccccgca cccatggaca gcagcaagcg cacctggctg
     2521 atcacgtcca gctgcgtggg cgcggccggc gccgcggccc tggcgacgcc gttcgtcacc
     2581 agctttgcgc cgtccgagcg ggccaaggcg gccggtgcgg cggtcgaggt cgacatcgcc
     2641 gggctcaagc cgggcgagaa gctcacggtc gaatggcgcg gcaagccggt ctggatcatg
     2701 cggcgcacgc cggagcagat ggcagcgct
//
LOCUS       DAOOOG010000267         2724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_45743_length_2724_cov_30.643687, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000267 DAOOOG010000000
VERSION     DAOOOG010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45743_length_2724_cov_30.643687"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PK242_13125"
     CDS             <1..868
                     /locus_tag="PK242_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HOZ95006.1"
                     /translation="QRRIGPLEGFRSKAGWPFVAELALVRDEAAGQWKLEFDFGDDAR
                     AEASGEIVDLSDQPSLGACPKCGGAVKAYGKSYLCERSLPSHAQPTPSCDFKSGQVIL
                     QQPVEPEQMRKLLAEGRTDVLDRFVSLRTRRPFKARLAWNADEGKVGFEFEPREGGRK
                     YPPRPSTASKTIAAGAALAGAVARKSAKKATAAGTAAKTRAPKADKAPKAPRAGNLHP
                     SPELAAVIGPGPFGRGEVMKKLWDYIKAHGLQDPQDKRSIVADDKLRPILGAERVGMF
                     KLAGLAGAHLKA"
     gene            918..1385
                     /locus_tag="PK242_13130"
     CDS             918..1385
                     /locus_tag="PK242_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOZ95007.1"
                     /translation="MNITQDTAVTLDYRITDAQGKPLDAGTTAYLHGGYDNLFAKAEA
                     ALQGKTVGDKVALALSAADAFGARDEALARSIPKSEFPPGVKVGGQLQGAGPHGPVVY
                     RVVKIKGPEVLLDANHPLAGLDIRFVATVKAVRAATAEEIAHRHVHGAHGHHH"
     gene            1514..>2724
                     /locus_tag="PK242_13135"
     CDS             1514..>2724
                     /locus_tag="PK242_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ95008.1"
                     /translation="MAPASDPHAAAPPLAARQAWAMLLALTGAFVLSQAFRTLGAIMG
                     PPLAAQLQLSAQQLGLWSAAFHFSFGAMQLAMGVSLDLWGVRRTILLAFPFAAAGALL
                     SSQVDGFGMLLLGQALIGAGCAPAFLVCTVFIGRHFAPERFTAMSGLTMSLSGLGVLA
                     TASPLAWLIERSHWRWGFGVLAGLSVLVWLVIAWRVHEPARPADAGAPRPTALDALRG
                     LLGLFALPQTAGVVAYAAVGYAAFITLRGLWLGPLLVQRFDLSLLQAGHVALAMTLGS
                     MASPAIFGRLDPGGARRLRWLLTLATVAALLLATIGLVPLLWLDVLLAGSYGLISGFG
                     VLQYGYVNDAYPPALRGRAMSLLTMAMFLGVSLMQWASGLAASLAPKLGVEPFSAALL
                     TMAAMLLAGALA"
BASE COUNT          369 a          987 c          980 g          388 t
ORIGIN      
        1 ccagcgccgc atcggcccgc tggaaggctt tcgcagcaag gccggctggc ccttcgtggc
       61 cgaactggcg ctggtgcgcg acgaggccgc gggccagtgg aagctggagt tcgacttcgg
      121 cgacgacgcc cgcgccgaag ccagcggcga gatcgtcgat ctgtccgacc agcccagcct
      181 gggcgcctgt cccaagtgcg gcggcgccgt caaggcctac ggcaagagct acctgtgcga
      241 acgcagcctg ccgtcgcacg cgcagcccac gcccagttgc gacttcaaga gcgggcaggt
      301 gatcctgcag cagccggtcg aacccgagca gatgcgcaag ctgctggccg agggccgcac
      361 cgacgtgctc gaccgcttcg tcagcctgcg cacgcgccgc ccgttcaagg cgcgcctggc
      421 ctggaacgcc gacgaaggca aggtgggctt cgagttcgag ccgcgcgaag gcggccgcaa
      481 atacccgcca cgcccctcga ccgcttccaa aacaatagct gctggcgcag cactggcggg
      541 cgctgtggcc aggaaatccg ccaagaaagc gaccgccgcc ggaaccgccg ccaagacccg
      601 ggctcccaag gccgacaagg cccccaaggc cccgcgcgcc ggcaacctgc accccagccc
      661 cgaactggcc gccgtcatcg ggccgggacc gttcggccgc ggcgaggtga tgaagaagct
      721 gtgggactac atcaaggccc acggcctgca ggatccgcag gacaagcgca gcatcgtcgc
      781 cgacgacaag ctcaggccca ttctgggcgc cgagcgcgtg ggcatgttca agctggccgg
      841 cctggcgggc gcgcacctga aggcctgagc ggcgctgcgc gcccttctcc cgtaccgacc
      901 acccatcacg caccaccatg aacatcaccc aggacaccgc cgtcacgctc gactaccgca
      961 tcaccgacgc ccagggcaag ccgctggacg ccggcaccac cgcctacctg cacggcggct
     1021 acgacaacct gttcgccaag gccgaagcgg cgctgcaagg caagaccgtg ggcgacaagg
     1081 tggcgctggc gctgagcgcg gccgacgcct tcggcgcgcg cgacgaggcc ctggcgcgca
     1141 gcatcccgaa aagcgagttt ccgcccggcg tcaaggtggg cggccagttg cagggcgccg
     1201 ggccgcacgg gccggtggtc taccgcgtgg tcaagatcaa gggccccgag gtgctgctgg
     1261 acgccaacca cccgctggcc ggcctggaca tccgcttcgt cgccaccgtc aaggcggtgc
     1321 gcgcggccac cgccgaggag atcgcgcacc gccacgtgca cggtgcgcat gggcatcacc
     1381 actgaccgca aagctcctga atgcatagct gctggcgctt gccagacaag cgctgcaggc
     1441 caatgaaaca tatgaccaag gtggcgtgcc ggcgcgcgcc ggcgggcggc gcagcgccgg
     1501 ggccggaggc ctgatggcgc ctgcttcgga cccgcacgcc gcggcgccgc cgcttgccgc
     1561 ccgtcaagcc tgggccatgc tgctggcgct gaccggcgcc ttcgtgctca gtcaggcgtt
     1621 tcgcaccctg ggcgccatca tggggccgcc gctggcggcg cagctgcagc tcagtgcgca
     1681 gcagctgggg ctgtggtcgg cggcgtttca cttctcgttc ggcgccatgc agctggccat
     1741 gggcgtgtcg ctggacctgt ggggcgtgcg ccgcaccatc ctgctggcgt ttccgttcgc
     1801 ggcggcgggg gcgctgctgt cgtcgcaggt cgacggcttc ggcatgctgc tgctggggca
     1861 ggcgctgatc ggcgccggct gcgcgccggc gtttctggtg tgcacggtgt tcatcggccg
     1921 gcatttcgcg cccgagcgct ttaccgccat gtcgggcctg accatgagcc tgagcggcct
     1981 gggcgtgctg gccaccgcca gcccgctggc ctggctgatc gagcgcagcc actggcgctg
     2041 gggcttcggc gtgctggccg ggctgtcggt gctggtgtgg ctggtgatcg cctggcgcgt
     2101 gcacgagccg gcccgcccgg ccgacgccgg cgcgccgcgc cccaccgcgc tggacgcgct
     2161 gcgcggcctg ctgggcctgt ttgcgctgcc gcagacggcc ggcgtggtgg cctacgcggc
     2221 ggtcggctac gccgccttca tcaccctgcg cgggctgtgg ctggggccgc tgctggtgca
     2281 gcgcttcgac ctgtcgctgc tgcaggccgg ccatgtggcg ctggccatga cgctgggcag
     2341 catggccagc ccggccatct tcggccggct cgacccgggc ggcgcgcgcc ggctgcgctg
     2401 gctgctgacg ctggccacgg tggcggcgct gctgctggcc accatcggcc tggtgccgct
     2461 gctgtggctg gacgtgctgc tggccggcag ctacgggctg atctcgggct ttggcgtgct
     2521 gcagtacggc tacgtcaacg acgcctaccc gccggcgctg cgcgggcgcg ccatgtcgct
     2581 gctgaccatg gccatgttcc tgggcgtgtc gctgatgcaa tgggccagcg ggctggccgc
     2641 cagcctggca ccgaagctgg gcgtggagcc gttcagcgcc gccctgctga cgatggcggc
     2701 catgctgctg gccggcgcgc tggc
//
LOCUS       DAOOOG010000268         2705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_46181_length_2705_cov_26.607547, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000268 DAOOOG010000000
VERSION     DAOOOG010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46181_length_2705_cov_26.607547"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="PK242_13140"
     CDS             <1..131
                     /locus_tag="PK242_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HOZ95009.1"
                     /translation="QAAIQGDAVRVTGAKRDDLQAVMALLRKDMAELPLSFNNFRD"
     gene            139..792
                     /locus_tag="PK242_13145"
     CDS             139..792
                     /locus_tag="PK242_13145"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HOZ95010.1"
                     /translation="MAALCTPWRTLLAGALLLPTWAGAQNVSLHGLLGQRALLIVDDQ
                     APRAVAPGETHLGVQVLSTGDDRAVVRVGGQRLTLRVGESPASVGGAPAPAGSDRVAL
                     TADARGHFFVAGSINQRPTQFMVDTGASVVAIGQAEADRLGLNYRAGHSVTLRTANGN
                     APGWALKLASLRIGDVTAYEVDAVVTPAAMPAVLLGNSFLNRFNMRRDGATMLLVKR"
     gene            complement(850..1203)
                     /locus_tag="PK242_13150"
     CDS             complement(850..1203)
                     /locus_tag="PK242_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOZ95011.1"
                     /translation="MSAIQRHGVGARLSEAAVFNGVVYLAGMVPEGGARDIRAQTADV
                     LAQIDAQLAACGSDRTRILRAQIFLADIADIAAMNEVWDAWVVPGHAPPRATVQAALA
                     DPAWRIEVVVTAAQA"
     gene            complement(1200..1823)
                     /gene="plsY"
                     /locus_tag="PK242_13155"
     CDS             complement(1200..1823)
                     /gene="plsY"
                     /locus_tag="PK242_13155"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOZ95012.1"
                     /translation="MILPLLATLAAYLIGSLSFAVIVSRAMGLQDPRSYGSGNPGATN
                     VLRSGSKAAAILTLLLDAAKGWLPVVLVRAFGADLGLGAGTQALVGLAAFLGHLYPVF
                     FRFQGGKGVATAAGVLLAFAPWLGVATLVTWLIIAVFFRYSSLASIVAALFAPAYYLL
                     GDGVAWTASGYKTLALMVMGVLLIWRHRENIRRLLAGTESKLGSKKK"
     gene            complement(1903..2400)
                     /locus_tag="PK242_13160"
     CDS             complement(1903..2400)
                     /locus_tag="PK242_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HOZ95013.1"
                     /translation="MAKSKSAHVSETPATALLRAHGIAFTEHPYEYVEHGGAEHSAEV
                     LGFDPFSVVKTLIMQDQDARPLIVLMHGNRKVSTKNLARQIGAKSVEPCKPEVANRHS
                     GYLVGGTSPFGTRRQMSVYIEESILELPRIAINGGRRGYLVAIEPQVCVRLLGAQPVR
                     CAIEL"
     gene            complement(2460..>2705)
                     /locus_tag="PK242_13165"
     CDS             complement(2460..>2705)
                     /locus_tag="PK242_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOZ95014.1"
                     /translation="ALRPHQVSIADPQQAGDAARLWLDGQVESAEFLGEFSRYRVRVG
                     ELALLSDQPHYAGVPLFPAGAPVRLGIDPRQLRVLTS"
BASE COUNT          417 a          957 c          955 g          376 t
ORIGIN      
        1 tgcaggccgc catccagggc gacgcggtgc gcgtgaccgg cgccaagcgc gacgatctgc
       61 aggccgtgat ggcgctgctg cgcaaggaca tggcggagtt gccgctgtcg ttcaacaact
      121 tccgcgattg acgcacccgt ggcagcgctc tgcaccccct ggcgcaccct gctggccggc
      181 gccctgctgc tgccgacctg ggccggggcg cagaacgtgt cgctgcacgg gctgctgggc
      241 cagcgggcgc tgctgatcgt cgacgaccag gccccgcgcg ccgtggcgcc gggcgaaacg
      301 cacctgggcg tgcaggtgct gtcgaccggc gacgatcgag cggtggtgcg cgtcggtggc
      361 cagcgcctga ccctgcgcgt gggcgaatcg ccggccagcg tgggcggcgc acccgcgccc
      421 gccggcagcg accgggtggc gctgacggcg gatgcgcgcg ggcatttctt tgtcgccggc
      481 agcatcaacc agcggcccac gcagttcatg gtggacaccg gcgcatcggt ggtggccatc
      541 ggtcaggccg aggccgaccg gctgggcctg aactaccgcg ccggccacag cgtgaccctg
      601 cgcaccgcca acggcaacgc gcccggctgg gcgctcaagc tggccagcct gcgcatcggc
      661 gacgtcacgg cctacgaggt ggacgccgtc gtcaccccgg cggcgatgcc ggcggtgctg
      721 ctgggcaaca gctttctgaa ccgcttcaac atgcggcgcg acggcgccac gatgctgctg
      781 gtcaaacgct agtgcggcct ccagcgcttg ctcagcatgc gccagcagct attgtttcga
      841 tagtaacggt caggcctggg cggccgtcac caccacctcg atgcgccagg ccgggtcggc
      901 cagagcggcc tgcaccgtgg cgcgcggcgg cgcatggccg ggcaccaccc aggcgtccca
      961 gacctcgttc atggcagcga tgtcggcgat gtcggccaga aagatctgtg cccgcaggat
     1021 gcgcgtccgg tcgctgccgc aggcggccag ctgggcgtcg atctgggcca gcacgtcggc
     1081 cgtctgggca cggatgtcgc gggcaccgcc ttcgggcacc atgccggcca ggtagaccac
     1141 gccgttgaag accgccgcct cggacaggcg cgcgcccacc ccgtgtcgct ggatggcgct
     1201 catttcttct tgctccccag tttcgattcg gtgcccgcca gcaggcggcg gatgttctcg
     1261 cggtggcgcc agatcaacag cacacccatg accatcaggg ccagcgtctt gtagcccgag
     1321 gcggtccagg cgacgccgtc gcccagcagg tagtaggccg gcgcaaacag cgcggccacg
     1381 atcgaggcca gcgaggagta gcggaagaac acggcgatga tgagccaggt gaccagcgtg
     1441 gccacgccca gccagggcgc aaaggccaac agcacgccgg ccgccgtggc cacccccttg
     1501 ccaccctgga agcggaagaa caccgggtac aggtgcccca ggaaggccgc caggcccacc
     1561 agcgcctgcg tgcccgcgcc caggccgaga tcggcaccga acgccctcac cagcaccacc
     1621 ggcagccagc ccttggcggc gtcaagcaac agtgtgagga tggccgccgc cttggagccc
     1681 gagcgcagca cgttggtggc gccggggttg ccgctgccgt agctgcgcgg gtcctgcagg
     1741 cccatggcgc ggctgacgat gacggcgaag ctcagcgagc cgatcaggta ggcggccagg
     1801 gtcgccagca ggggcaggat catgcggtgg gtactcctga atcaatagct gcttgcgctt
     1861 gccagacaag cgcccgagcc gcattttgcc agcaatcgag catcacagct cgatcgcgca
     1921 ccgcaccggc tgggcgccca acaggcgcac gcacacctgc ggctcgatcg ccaccaggta
     1981 gccgcgccgc ccgccgttga tggcaatgcg cggcagctca agaatgcttt cctcgatgta
     2041 caccgacatc tgccggcgcg tgccaaacgg cgaggtgccg ccgaccagat agccgctgtg
     2101 gcggttggcg acctcgggct tgcaaggctc gacgctcttg gcgccgatct ggcgcgccag
     2161 gttcttggtg ctgaccttgc ggttgccgtg catcagcacg atcagagggc gcgcgtcctg
     2221 gtcctgcatg atgagcgtct tgaccacgct gaacgggtcg aaacccaaca cctcggcgct
     2281 gtgctcggcg ccgccgtgct cgacgtactc atagggatgc tcggtgaagg caatgccgtg
     2341 cgcgcgcagc agcgcggtgg ctggggtttc gctgacgtgg gcggacttcg acttggccat
     2401 ggcatgaacg gcgcggcacg gcgccggcag cgattgtgcc cgtccgccgg gccaccgcgt
     2461 caggacgtca gcacacgcag ctggcgcggg tcgatcccca ggcgcaccgg cgcgccggcg
     2521 ggaaacagcg gcacgccggc gtagtggggc tggtcgctca gcagcgccag ctcgcccacg
     2581 cgcacgcggt agcgcgagaa ctcgcccagg aactcggcgc tctccacctg cccgtcgagc
     2641 cacagccgtg cggcgtcgcc cgcctgctgc gggtcggcga tgctgacctg gtgcggccgc
     2701 agcgc
//
LOCUS       DAOOOG010000269         2693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_46477_length_2693_cov_39.153904, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000269 DAOOOG010000000
VERSION     DAOOOG010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46477_length_2693_cov_39.153904"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PK242_13170"
     CDS             <1..1322
                     /locus_tag="PK242_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ95015.1"
                     /translation="TSDYLGMLRELAPEWAHARPGEPLQSQTLPQLQHAIWIDVAGEG
                     AEEPGFMRFSELLASGDPQDPRLAQVARLLHPQDAINIQFTSGTTGFPKGATLTHRNI
                     LNNGFFIGEAMKLTEQDRLCIPVPLYHCFGMVLGNLAVLTHGGCIVYPNDAFDPLTVL
                     QTVQDEKCTGLHGVPTMFIAELDHPRFKEFDLSSLRTGIMAGSPCPIEVMKRVQSEMN
                     MGEVTIAYGMTETSPVSCQSSTDTPLDKRVSTVGLVQPHLEIKIIDPETGRTVPVGER
                     GEFCTRGYSVMQGYWEDPQRTAEAIDAEGWMHTGDLATMDGEGYVNIVGRIKDMVIRG
                     GENVYPREIEEFLYRHPKVQDVQVVGVPDQKFGEELCAWIIVKPGQQLTDAEVREFCK
                     GKIAHYKVPRYLKFVPEFPMTVTGKIQKFKIREAMVAELGLVQDQTA"
     gene            1472..>2693
                     /locus_tag="PK242_13175"
     CDS             1472..>2693
                     /locus_tag="PK242_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOZ95016.1"
                     /translation="MPAIDTKLNPRSADFQANAQAMRALVDDLRAQVRKVSEGGGEAA
                     RKKHMDRGKLLPRVRVQMLLDPGTPFLEIAPLAALHMYQDRNGQDEAPAAGMVAGIGR
                     VSGVDCMIVCNDATVKGGTYYPMTVKKHLRAQEIAQQNRLPCIYLVDSGGANLPNQDE
                     VFPDRDHFGRIFYNQAQMSAQGIAQIAVVMGSCTAGGAYVPAMSDESIIVRNQGTIFL
                     GGPPLVKAATGEEVSAEDLGGGDVHTRQSGVADHLAQNDMHALALARQVVAHLNRHKA
                     PAGDLRTPCPPRYAAEELYGVIPTDTRKPFDVREIIARIVDDSAFQEFKARFGTTLVC
                     GFAHIEGMPVGIVANNGILFSESAQKGAHFIELCCQRKIPLVFLQNITGFMVGRKYEN
                     EGIARHGAKMVTAVA"
BASE COUNT          509 a          908 c          869 g          407 t
ORIGIN      
        1 agaccagcga ctacctgggc atgctgcgcg agctggcgcc cgagtgggcg cacgccaggc
       61 cgggcgagcc gctgcagtcg cagacgctgc cgcagctgca gcacgcgatc tggatcgacg
      121 ttgccggcga aggcgccgag gagccgggct tcatgcgttt ttccgagctg ctggcgagtg
      181 gcgacccgca ggatccgcgc ctggcccagg tggcgcgcct gctgcatccg caggacgcca
      241 tcaacatcca gttcaccagc ggcaccaccg gctttcccaa gggcgccacg ctgacgcacc
      301 gcaacatcct gaacaacggc ttcttcatcg gcgaagcgat gaagctgacc gagcaggacc
      361 gcctgtgcat ccccgtgccg ctgtaccact gcttcggcat ggtgctgggc aacctggcgg
      421 tgctgacgca tggcggctgc atcgtctatc ccaacgacgc cttcgacccg ctcaccgtgc
      481 tgcagaccgt gcaggacgag aagtgcaccg gcctgcacgg cgtgcccacc atgttcatcg
      541 ccgagctgga tcacccgcgc ttcaaggaat ttgatctgtc cagcctgcgt accggcatca
      601 tggccggcag cccctgcccg atcgaggtga tgaagcgcgt gcagagcgaa atgaacatgg
      661 gcgaggtgac catcgcctac ggcatgaccg agacctcgcc cgtcagctgc cagagttcca
      721 ccgacacgcc gctggacaag cgcgtgtcca ccgtgggcct ggtgcagccg cacctggaga
      781 tcaagatcat cgaccccgag accggccgca ccgtgccggt gggcgagcgc ggcgagttct
      841 gcacccgcgg ctattcggtg atgcagggct actgggaaga cccgcagcgc accgccgagg
      901 cgatcgacgc cgaaggctgg atgcacaccg gcgatctggc gaccatggac ggcgagggct
      961 acgtcaacat cgtcggccgc atcaaggaca tggtgatccg cggcggcgaa aacgtctatc
     1021 cgcgcgagat cgaagagttc ttgtaccgcc accccaaggt gcaggacgtg caggtggtgg
     1081 gcgtgccgga ccagaaattt ggcgaggagc tgtgcgcctg gatcatcgtc aagcccggcc
     1141 agcagctcac cgacgccgag gtgcgcgagt tctgcaaggg caagatcgcg cactacaagg
     1201 tgccgcgcta cctgaagttc gtgcccgagt tccccatgac cgtgaccggc aagatccaga
     1261 aattcaagat ccgcgaggcc atggtggccg agctcggtct ggtgcaggac cagacggcct
     1321 gaaatccaaa actggaccga acgcagcgaa cgcaaaattg acgcagagga cgcaagagag
     1381 cagcccattt gattcagcat tttttgtgcg ccctctgcga aacctttgcg ccctttgcgt
     1441 tcggtattca attttcattt gagccccgcc catgcccgcc atcgacacca aactgaaccc
     1501 acgctcggcc gacttccagg ccaacgccca ggccatgcgc gcgctggtgg acgatctgcg
     1561 cgcccaggtg cgcaaggtca gcgaaggcgg cggcgaggcg gcgcgcaaga agcacatgga
     1621 ccgcggcaag ctgctgccgc gcgtgcgcgt gcagatgctg ctcgaccccg gcacgccctt
     1681 tctggaaatc gccccgctgg cggcgctgca catgtaccag gaccgcaacg gccaggacga
     1741 ggcgcccgcc gccggcatgg tggcgggcat cggtcgcgtt tcgggcgtgg actgcatgat
     1801 cgtctgcaac gacgccacgg tgaagggcgg cacctactac cccatgacgg tgaaaaagca
     1861 cctgcgcgcg caggagatcg cgcagcaaaa ccgcctgccc tgcatctacc tggtcgacag
     1921 cggcggcgcc aacctgccca accaggacga ggtgtttccc gaccgcgacc acttcggccg
     1981 catcttctac aaccaggcgc agatgagcgc ccagggcatt gcgcagatcg ccgtcgtcat
     2041 gggttcctgc actgccggcg gcgcctatgt gccggcgatg agcgacgagt ccatcatcgt
     2101 caggaaccag ggcaccatct ttctgggcgg cccgccgctg gtcaaggccg ccaccggcga
     2161 ggaggtgagc gccgaagacc tgggcggcgg cgacgtgcac acccgccagt cgggcgtggc
     2221 cgaccacctg gcgcagaacg acatgcatgc gctggcgctg gcgcggcagg tggtggcgca
     2281 cctgaaccgc cacaaggcgc cggcgggcga tctgcgcaca ccctgtccgc cccggtacgc
     2341 cgccgaggag ctgtacggcg tgatccccac cgacacccgc aagccgttcg acgtgcgcga
     2401 aatcatcgcc cgcatcgtcg atgacagcgc gttccaggag ttcaaggcgc gctttggcac
     2461 caccctggtg tgcggctttg cccacatcga gggcatgccg gtgggcatcg tggccaacaa
     2521 cggcatcctg ttttccgagt cggcgcaaaa gggcgcgcac ttcatcgagc tgtgctgcca
     2581 gcgcaagatt ccgctggtgt tcctgcagaa catcaccggc ttcatggtcg ggcgcaagta
     2641 cgagaacgaa ggcatcgccc gccacggcgc caagatggtg acggccgtgg cca
//
LOCUS       DAOOOG010000270         2690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_46535_length_2690_cov_24.365085, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000270 DAOOOG010000000
VERSION     DAOOOG010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46535_length_2690_cov_24.365085"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2198
                     /gene="gspD"
                     /locus_tag="PK242_13180"
     CDS             <1..2198
                     /gene="gspD"
                     /locus_tag="PK242_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HOZ95017.1"
                     /translation="SGGKVTLDFANAEIDAVARTMATLTGRNVVVDPRVRGTLTLTST
                     TPVSPAEALRLFGAQLRTQGFALVENAGMVLVLPEGEAKLQGGSVSAGAVRGGGQIQT
                     QIFRLTHENANNLVPILRPLISPNNTINVGPGTNALVITDYGDNLQRLGRIIAALDVS
                     SATGVEVVRLQHAVASDLAPLVARLLESGGGGSVATPAPVPGQPGMPAAAAALAGSEG
                     GFRTTLVPEPRSNAIVVRAANPARLALARTLIEQLDQPPARRPDGGSGNIHVVYLKNS
                     DAAKLAVTLRAALAALPGQSSATGAAAAGGGASASAATGQGLTAQLSAAGGTLGGNGL
                     ASGPTIDARAANQPSTGGQIQADPATNSLIISAPEPVYRELRAVIDKLDQRRAQVFVE
                     SLIAEVRADRAADFGVQWQSVLRNNGSTIGVIGTNYSTTRATGGSTTNIFDIAAAVLG
                     YRSSGNVSDLAGAVPNTGFNLALGSRTLGLLANFIQSSGAGNVLSTPTLLTLDNEEAK
                     IIVGQNVPFVTGSYTNTGSSSTSVNPFQTFERKDVGLTLRVRPQISEDGTIKMVIYQE
                     TSSVVASSVGSSQGPITNKRAIESSVLVNDGSVVVLGGLLEDQFATGEDKVPGLGDIP
                     LLGNLFKNQTRSYVKTNLMVFLRPVIVRDGAATEAFSLDRYDYMRAAQSASQPEPSSV
                     LQINQAPVLPAVQPVNPAAWTRPAPPPPLIRPAAGEHSDPGGGTTFSKP"
     gene            2207..>2690
                     /locus_tag="PK242_13185"
     CDS             2207..>2690
                     /locus_tag="PK242_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOZ95018.1"
                     /translation="MRYPLPYAFARTAGLLLEEGGGQRTLWHGGAPDPGALSEVLRRH
                     GGGEPPLVLQALDAGALAQRISHAYAQSESSAAAVVSEVESDADLSRIMQELPAVEDL
                     LEAADDAPIIRMLNALLTQAARDGASDIHIEPYERHSSVRFRVDGALREVVQPNRALH
                     A"
BASE COUNT          427 a         1047 c          903 g          313 t
ORIGIN      
        1 ccagcggcgg caaggtgacg ctggacttcg ccaacgccga gatcgacgcc gtggcgcgca
       61 ccatggccac gctgaccggc cgcaacgtgg tggtcgaccc acgcgtgcgc ggcacgctga
      121 cgctgaccag caccacgccg gtctcgcccg ccgaggcgct gcgcctgttc ggcgcccagc
      181 tgcgcaccca gggctttgcg ctggtcgaaa acgccggcat ggtgctggtg ctgcccgagg
      241 gcgaggccaa actgcagggc ggcagcgtgt cggccggcgc ggtgcgcggc ggcggccaga
      301 tccagaccca gatctttcgc ctgacgcacg agaacgccaa caacctggtg cccatcctgc
      361 gcccgctgat cagccccaac aacaccatca acgtcgggcc cggcaccaac gcgctggtga
      421 tcaccgacta cggcgacaac ctgcagcggc tgggccgcat catcgccgcg ctggacgtgt
      481 ccagcgccac cggggtcgag gtggtgcgcc tgcagcacgc cgtggcctcc gacctggcgc
      541 cgctggtggc gcgcctgctc gaatcgggcg gcggcggcag cgtcgccacc ccggcgccgg
      601 tgccgggcca gcccggcatg cccgcggcgg ccgccgcgct ggccggcagc gaaggcggct
      661 ttcgcaccac cctggtgccc gagccgcgca gcaacgccat cgtcgtgcgc gccgccaacc
      721 cggcgcggct ggcgctggcg cgcacgctga tcgagcagct cgaccagccg ccggcgcgcc
      781 ggcccgatgg cggcagcggc aacatccacg tcgtgtacct gaagaattcc gacgccgcca
      841 agctggccgt cacgctgcgc gccgcgctgg cggccctgcc gggccagagc agcgcgacgg
      901 gcgcggccgc cgcgggtggc ggcgccagcg ccagcgccgc caccggccag gggctgacgg
      961 cccagctcag cgccgccggc ggcacgctcg gcggcaacgg cctggccagc ggcccgacga
     1021 tcgacgcccg cgccgccaac cagccgtcca ccggcggcca gatccaggcc gacccggcca
     1081 ccaactcgct catcatcagc gcgcccgagc cggtgtaccg cgagctgcgc gccgtcatcg
     1141 acaagctcga ccagcgccgc gcccaggtct tcgtcgaaag cctgatcgcc gaggtgcgcg
     1201 ccgaccgggc ggccgacttc ggcgtgcagt ggcagagcgt gctgcgcaac aacggcagca
     1261 ccatcggcgt catcggcacc aactattcga ccacccgcgc caccggcggc agcaccacca
     1321 acatcttcga catcgcggcg gcggtgctgg gctaccgcag cagcggcaac gtctccgacc
     1381 tggcgggagc cgtccccaac accggcttca acctggcgct gggttcgcgc accctgggcc
     1441 tgctggccaa cttcatccag agcagcggcg ccggcaacgt gctgtccacg cccaccctgc
     1501 tgacgctgga caacgaagaa gccaagatca tcgtcggcca gaacgtgccc ttcgtcaccg
     1561 gcagctacac caacaccggc agcagcagca cctccgtcaa cccgttccag accttcgagc
     1621 gcaaggacgt cggcctgacg ctgcgcgtgc gcccgcagat cagcgaagac ggcaccatca
     1681 agatggtgat ctaccaggaa acctccagcg tggtggccag ctcggtcggc tcgtcgcagg
     1741 ggccgatcac caacaagcgc gccatcgaat cgagcgtgct ggtcaacgac ggctcggtgg
     1801 tggtgctggg cgggctgctg gaagaccagt tcgccaccgg cgaggacaag gtgcccggcc
     1861 tgggcgacat cccgctgctg ggcaacctgt tcaagaacca gacgcgcagc tacgtcaaga
     1921 ccaacctgat ggtgtttctg cgcccggtca tcgtgcgcga cggcgcggcc accgaagcct
     1981 tctcgctcga ccgctacgac tacatgcgcg ccgcccagtc ggccagccag cccgagccca
     2041 gcagcgtgct gcagatcaac caggcgccgg tgctgccggc ggtgcagccg gtcaacccgg
     2101 ccgcctggac gcgcccggcg ccgccgccgc cgctgatccg tccggccgcc ggcgaacaca
     2161 gcgaccccgg cggcggcacc accttcagca agccgtagcc gcggccatgc gctacccgct
     2221 gccctacgcc ttcgcgcgca ccgccggtct gctgctggaa gaaggcggcg ggcagcgcac
     2281 gctgtggcac ggcggcgcgc ccgaccccgg cgccctgagc gaagtgctgc gccgccacgg
     2341 cggcggcgag ccgccactgg tgctgcaggc gctcgacgcc ggcgcgctgg cccagcgcat
     2401 cagccatgcc tatgcgcaaa gcgaatccag cgccgccgcg gtggtcagcg aagtcgagtc
     2461 cgacgccgac ctgtcgcgca tcatgcagga gctgccggcg gtggaagacc tgctggaagc
     2521 cgccgacgac gcgcccatca tccgcatgct caacgccctg ctgacgcagg ccgcgcgcga
     2581 cggcgccagc gacatccaca tcgagcccta cgagcgccat tccagcgtgc gctttcgcgt
     2641 cgacggcgcg ctgcgcgagg tggtgcagcc caaccgcgcg ctgcacgcgg
//
LOCUS       DAOOOG010000271         2671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_46998_length_2671_cov_27.097095, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000271 DAOOOG010000000
VERSION     DAOOOG010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46998_length_2671_cov_27.097095"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2643
                     /locus_tag="PK242_13190"
     CDS             <1..2643
                     /locus_tag="PK242_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOZ95019.1"
                     /translation="LTAFAQAAQVGGAARAFEALAGRPYQSEPFGPDVPCVCLRIPTG
                     GGKTLLAAHAVPLLARHWRASDAPVAVWLVPSDAIRAQTLQGLQTPGHPVRQVLADAY
                     GERLRVCALEDVAQIAPPDWGRAAVVVVATIQSFRIDDTDKRNVYSFAESFERHFKAA
                     QSEQPRALAALAALPGACVTADEAAQDGRALLRGFVGQPRQSLANWLALHQPIVIVDE
                     AHNAKTDKSFTALKRLNPACLLELTATPIAARTNVLYHVSAQELAAEHMIKLPIALAE
                     HPEGWPQAVLATVRTRAALEAEALKDEAAGAGYVRPIALYQAQNAGDAVPPEALRRHL
                     IDELHVPPEQVVVATGSTRELDGLELAARDCPVRHVITVQALREGWDCPFAYVLCSLQ
                     KLASATAVEQLLGRVLRMPYATRRGREPLNRAWAHVCEAEFSSAAHALADRLIQGMGF
                     EALDVASMLAPAASLPLFEENQAPAPVQQAPVATDFESIAPTDALAALPGVAVRTIAG
                     APRVVVSGHIGAELEGQLLAGVRGAKKQDAVRQQIARHNALVAAQTAPAARGEHFAPV
                     PPLGYREPSQSELWPLEREALLEAVDIDLLTPEAIELPGFQVVEQGRAFEIYLKDEQV
                     RLRPAEASQIALDYGVSGIGAQDLVRWLEQSLYASLPEFTPAQRQPWFAAVVNRLLHE
                     QRVPIETLAQARFTLARALQAKAADLRDAAAKARFHQLVLDGGWQVEPDWARPHVFGP
                     ATYPAPAGSRYGGRYEFRKHYYPVIADLKSEGEEFACAQLIDRHPKVKHWVRNLDAAP
                     AGFWLPTSRRRFFADFVAELVDGRIALLEYKGAHLASDPGEIEKRQIGELWARRSGGR
                     AVFGWLMKAQDGLGPAQQLDAVLA"
BASE COUNT          374 a          931 c         1013 g          353 t
ORIGIN      
        1 ctgaccgcct ttgcccaggc ggcgcaggtg gggggcgcgg cgcgggcgtt cgaggcgctg
       61 gcggggcggc cgtaccagag cgagccgttc gggcccgacg tgccctgcgt gtgcctgcgc
      121 atccccaccg gcggcggcaa gacgctgctg gcggcgcacg ccgtgccgct gctggcgcgg
      181 cactggcgcg ccagcgacgc gccagtggcg gtgtggctgg tgcccagcga cgccatccgg
      241 gcgcaaaccc tccagggtct gcaaacgccc ggccacccgg tgcgccaggt gctggccgac
      301 gcctatggcg agcgcctgcg cgtgtgcgcg ctggaggacg tggcgcagat cgccccgccc
      361 gactggggcc gcgccgcggt ggtggtggtg gccacgatcc agagctttcg catcgacgac
      421 acggacaagc gcaacgtcta cagctttgcc gagtcgttcg agcgccactt caaagccgcc
      481 cagagcgagc agccgcgcgc gctggcggcg ctggccgcgc tgccgggcgc ctgcgtgacg
      541 gccgacgagg cggcgcagga cggccgcgcc ctgctgcgcg gctttgtcgg ccagccgcgc
      601 cagagcctgg ccaactggct ggcgctgcac cagccgatcg tgatcgtcga cgaggcgcac
      661 aacgccaaga ccgacaagag cttcacggcg ctcaagcgcc tgaacccggc ctgcctgctg
      721 gagctgacgg ccacgccgat cgcggcgcgc accaacgtgc tctaccacgt gagcgcgcag
      781 gagctggcgg ccgagcacat gatcaagctg ccgatcgcgc tggccgagca ccccgagggc
      841 tggccgcagg cggtgctggc gacggtgcgc acgcgcgccg cgctggaggc cgaggcgctg
      901 aaggacgagg ccgccggcgc cggctacgtg cggccgatcg cgctgtacca ggcgcagaac
      961 gcgggcgacg ccgtgccgcc cgaggcgctg cgccggcacc tgatcgacga gctgcacgtg
     1021 ccgcccgagc aggtggtggt ggccaccggc agcacgcgcg agctggacgg gctggagctg
     1081 gccgcgcgcg actgcccggt gcgccacgtc atcaccgtgc aggcgctgcg cgaaggctgg
     1141 gactgcccgt ttgcctatgt gctgtgctcg ctgcaaaagc tggccagcgc gacggcggtg
     1201 gagcagttgc tgggccgcgt gctgcgcatg ccttacgcca cgcggcgcgg gcgcgagccg
     1261 ctcaaccgcg cctgggcgca tgtgtgcgag gccgagtttt ccagcgcggc gcacgcgctg
     1321 gccgaccggt tgattcaggg catgggcttc gaggcgctgg acgtggcctc gatgctggca
     1381 cccgccgcat cgctgccgtt gtttgaagaa aatcaggccc cagcgcccgt ccagcaagca
     1441 ccagtagcta ctgattttga atcgatcgca ccgaccgacg cactggcggc gctgcccggc
     1501 gtggcggtgc ggaccatcgc cggcgcgccg cgcgtggtgg tcagcggcca catcggcgcc
     1561 gagctggagg gccagttgct ggccggcgtg cgcggcgcca agaagcagga cgccgtgcgc
     1621 cagcagatcg cgcggcacaa cgcgctggtg gcggcgcaaa cggccccggc cgcgcgcggc
     1681 gagcactttg cgccggtgcc cccgctgggg taccgggaac cttcccagtc cgagctgtgg
     1741 ccgctggagc gcgaggcgct gctggaggcg gtggatatcg acctgctgac gcccgaggcg
     1801 atcgagctgc ccggctttca ggtggtcgag caaggccgcg cgttcgagat ttacctgaag
     1861 gacgagcagg tgcggctgcg cccggccgag gccagccaga tcgcgctgga ctatggcgtc
     1921 agcggcatcg gcgcgcagga cctggtgcgc tggctggagc agtcgctgta cgccagcctg
     1981 cccgagttca cgccggcgca gcgccagccg tggtttgctg ccgtggtcaa ccgcctgctg
     2041 cacgagcagc gcgtgccgat cgagacgctg gcgcaggcgc gcttcacgct ggcgcgggcg
     2101 ctgcaggcca aggccgccga cctgcgcgac gcggcggcca aggcgcggtt ccatcaattg
     2161 gtgctggacg gcggctggca ggtcgagccc gactgggcgc gcccgcacgt gttcgggccc
     2221 gcgacctacc ccgcgccggc cggcagccgc tacggcgggc gctacgaatt tcgcaagcac
     2281 tactacccgg tgatcgccga cctgaaaagc gagggcgagg aatttgcctg cgcgcaactg
     2341 atcgaccgcc accccaaggt gaagcactgg gtgcgcaacc tggacgcggc gcccgcgggc
     2401 ttctggctgc cgacctcgcg ccggcgcttc tttgccgact tcgtggccga gctggtggac
     2461 gggcgcatcg ccctgctcga atacaagggc gcgcacctgg ccagcgaccc aggcgagatc
     2521 gaaaagcgcc agatcggcga gctgtgggcg cgccgcagcg ggggccgcgc cgtgttcggc
     2581 tggctgatga aggcgcagga cggcctgggc ccggcgcagc aactggacgc ggtgctggca
     2641 tgacgctgga ggtgcgcttt ctgggccgcg t
//
LOCUS       DAOOOG010000272         2659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_47299_length_2659_cov_22.015361, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000272 DAOOOG010000000
VERSION     DAOOOG010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47299_length_2659_cov_22.015361"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PK242_13195"
     CDS             <1..706
                     /locus_tag="PK242_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOZ95020.1"
                     /translation="DGVWGAWPGLGIALAVLAVTGLPHAFNIIDGYNGLAGSVALVVC
                     LALAHVALQVGDRELAAMVVTLAAAIAGFLVWNYPRGLIFAGDAGAYLWGLLIAAISL
                     LLVQRHPIVSPWFPMLLLIYPVWETVFSIYRKLARGDSPGMADALHLHQLVYRRLVRS
                     VLHDDEAQSLLKRNNRTTPYLWGFMVLSVAPALLFWRYSAVLLAFCVLFAVCYVWAYL
                     TLIRFKVPRVLQPPRE"
     gene            749..1642
                     /gene="argB"
                     /locus_tag="PK242_13200"
     CDS             749..1642
                     /gene="argB"
                     /locus_tag="PK242_13200"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOZ95021.1"
                     /translation="MTTDTTALSPRDKAEILAQALPYIRRYHGRTMVIKYGGNAMTDP
                     ALQQDFAEDVVLLKLVGINPVVVHGGGPQIEAALKRLGKQGQFIQGMRVTDAETMEVV
                     EWVLAGEVQQDIVGLIHQAGGKAVGLTGRDGGLIHARKLKLLDQKDPRIEHDVGQVGD
                     IVAIDPAVVRALQDDQFIPVVSPIGFGEHNESYNINADVVAARLAMELQAEKLLMLTN
                     IPGVLDKAGALLPELTPRQIDALVDDGTISGGMIPKISGALDAARSGVKAVHIIDGRV
                     PHALLLEVLGDQPFGTMIRAQ"
     gene            1773..2420
                     /gene="slmA"
                     /locus_tag="PK242_13205"
     CDS             1773..2420
                     /gene="slmA"
                     /locus_tag="PK242_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HOZ95022.1"
                     /translation="MPAAFDPPTDDTDPAAQALAAPRKRPKPGERRVQILQALAQMLE
                     QPGAERVTTAALAARLSVSEAALYRHFASKAQMFEALIDFIEGSVFGLIGQITEREPD
                     GRTQAARIVAMVLQFGERNPGMARVMGGDALVLEHERLQQRMNQLFDKLEAALRQCLR
                     NAAAGGPATPTPDADAQVTASVLAAFTAGRLQRFARSGFRRAPTEQLDACIARML"
BASE COUNT          420 a          879 c          925 g          435 t
ORIGIN      
        1 cgatggcgtc tggggtgcct ggccggggct gggcatcgcg ctggcggtgc tggcggtgac
       61 cggtctgccg cacgccttca acatcatcga tggctacaac ggcctggccg gctcggtggc
      121 gctggtggtg tgtctggcgc tggcgcatgt ggcgttgcag gtcggtgacc gtgaactggc
      181 cgccatggtg gtgacactgg cggcggcgat cgcgggtttt ctggtgtgga actacccgcg
      241 cggcctgatc ttcgccggcg atgccggcgc ctacctgtgg ggcctgctga tcgccgccat
      301 cagcctgctg ctggtgcagc gccaccccat cgtgtcgccc tggtttccga tgctgctgct
      361 gatctacccg gtgtgggaga cggtgttctc catttaccgc aagctggcgc gcggcgactc
      421 gcccggcatg gccgacgcgc tgcacctgca ccagctggtg taccggcgcc tggtgcgcag
      481 cgtgctgcac gacgacgaag cgcagagcct gctcaagcgc aacaaccgca ccacgccgta
      541 cctgtggggc ttcatggtgc tgagcgtggc gccggcgctg ctgttctggc gctacagcgc
      601 ggtgctgctg gcgttctgcg tgctgttcgc cgtctgctac gtgtgggcct atctgacgct
      661 gatccgcttc aaggtgccgc gcgtgctgca gccaccgcgc gagtgacccc tatgatcggg
      721 gtttcgcacg tcttcatcgt cccccgccat gaccaccgac accaccgccc tttccccgcg
      781 cgacaaggcc gagatcctgg cccaggcact gccctacatc cgccgctatc acgggcgcac
      841 catggtcatc aagtacggcg gcaacgccat gaccgacccg gcgctgcagc aggacttcgc
      901 cgaggacgtg gtgctgctca agctggtggg catcaacccg gtggtggtgc atggcggcgg
      961 cccgcagatc gaagcggcct tgaagcgcct gggcaagcag ggccagttca tccagggcat
     1021 gcgcgtgacc gacgccgaga ccatggaggt ggtcgaatgg gtgctggccg gcgaggtgca
     1081 gcaggacatc gtcggtctga tccaccaggc cggcggcaag gcggtgggcc tgaccgggcg
     1141 cgacggcggc ctgatccacg cccgcaagct caagctgctg gatcagaagg atccccgcat
     1201 cgagcacgac gtgggccagg tgggcgacat cgtggccatc gacccggcgg tggtgcgcgc
     1261 gctgcaggac gaccagttca tcccggtggt cagcccgatc ggctttggcg agcacaacga
     1321 gagttacaac atcaacgccg acgtggtggc cgcgcgcctg gccatggagc tgcaggccga
     1381 gaagctgctg atgctgacca acatccccgg cgtgctggac aaggccggcg ccctgctgcc
     1441 cgagctgacg ccacgccaga tcgacgccct ggtggacgac ggcaccatct ccggcggcat
     1501 gatccccaag atcagcggtg cgctggacgc cgccaggagc ggcgtgaagg cggtgcacat
     1561 catcgacggc cgggtgccgc acgcgctgct gctggaggtg ctgggcgacc agcccttcgg
     1621 caccatgatc cgcgcgcaat aagccccggg gcgcgctgcc ctgggcaagc gctcacttga
     1681 cgccagtgtg gcgccaagca aacgcgggca cgcccgggct ttgaccggac gcgggcttgt
     1741 gcgagaatca tttggaaaca attgatgccg ccatgcccgc cgccttcgat cctccgaccg
     1801 acgacaccga cccagcggcg caggcgcttg ccgcgccgcg caagcgcccg aagccgggtg
     1861 agcgccgggt gcagatcctg caggcgctgg cccagatgct ggagcagcct ggcgccgagc
     1921 gcgtgaccac cgccgcgctg gccgcgcgtc tgtcggtgag cgaggcggcg ctgtaccgcc
     1981 acttcgccag caaggcgcag atgttcgagg cgctgatcga cttcatcgaa ggcagcgtgt
     2041 tcggcctgat cggccagatc accgagcgcg agccggacgg gcgcacgcag gccgcgcgca
     2101 tcgtggccat ggtgctgcag ttcggcgaac gcaacccggg catggcgcgg gtgatggggg
     2161 gcgacgcgct ggtgctggag catgaacgcc tgcagcagcg catgaaccag ctgttcgaca
     2221 aactggaggc ggcgctgcgc cagtgcctgc gcaacgccgc cgcgggtggg ccggccacgc
     2281 cgacgcccga tgccgacgcc caggtgacgg cctcggtgct ggccgccttc acggcggggc
     2341 ggctgcaacg cttcgcgcgc tcgggcttcc ggcgcgcgcc gaccgagcag ctggatgcct
     2401 gcattgccag aatgctataa aaatagaagc tgctggcgct cgaccagcgg gcgcaatcgg
     2461 ccgatttggg attcttctcg cgaatgatgg tgatggcctg cgagacaagg cttcgaatgg
     2521 tcgcttattg gaaaccgcca acctccgaac gcagaggacg cagagatttc gcagaggacg
     2581 cagaaagcca gaagatccat cggttttctt tgcatcctct gcgaaatctc tgcgtcccct
     2641 gcgtatggct gttcatttt
//
LOCUS       DAOOOG010000273         2646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_47601_length_2646_cov_26.084909, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000273 DAOOOG010000000
VERSION     DAOOOG010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47601_length_2646_cov_26.084909"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PK242_13210"
     CDS             complement(<1..358)
                     /locus_tag="PK242_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HOZ95023.1"
                     /translation="MKILASDALPTSAEAEKTLQIANDANLDELAASGAFEGVERIEL
                     QFPKFTDGRAYSQALLLRRRYKFAGDIRATGDVLIDQLVHMHRSGFSSAVLAEGVDAS
                     AAQRQFERFAAFYQGDV"
     gene            complement(385..2169)
                     /locus_tag="PK242_13215"
     CDS             complement(385..2169)
                     /locus_tag="PK242_13215"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HOZ95024.1"
                     /translation="MYQYTDFDKQFVKLRAQQFRDQLERWQRGELTDEQMLPLRLQNG
                     WYIQRYAPMARIAVPYGEISSLQLRTLGRIAREYDQPTPELLAHAQATQDALEASQPG
                     ATLAAKPLKYGYGHFTTRTNCQFNWIPITKAADVMDLLASVHMHGIQTSGNDIRNITC
                     DAWEGIAEDGIVDCRPFAEITRQWSSLHPEFSYLPRKFKIAFNGAVEDRAAIGWYDIG
                     LQALKNEAGEVGFTVKVGGGMGRTPIIGTTVREFLPWAELLNYIEAIVRVYNGYGRRD
                     NKWKARIKILVKSEGQGFIDAVEKEYRDIVELDGAPHRITQAELDRVASHFVVPELKP
                     ANLPSKVGPQGQLYRRWLQQNVRGHRLAGMKTVTLSFKRVGFAPGDADADTMDALAQL
                     AEQFSAGEARLTHEQNLLLPWVFESDLPALYEAARKLGLAQPNIGLLTDMIACPGGDF
                     CSLANARSLHIAAAVTERYQDLDELFDLGPIDLHMSGCINSCGHHHSGHIGILGVDKD
                     GKEWYQITLGGADGSQLSGPAIGGKVVGPSFTAAEVPGVIEAILDTYRELRQPGEFFI
                     DALRRIGHDPFKQAANAARHAKAEEALH"
     gene            complement(2196..>2646)
                     /locus_tag="PK242_13220"
     CDS             complement(2196..>2646)
                     /locus_tag="PK242_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOZ95025.1"
                     /translation="ATFYKVLVFSPERAAAEAARRSAQFAHTSAPRHWSLPVLLGAVI
                     GMLVTFTSVGAGAIGVSVLLLVFPHLPLPRIIAADIAYAVPLTLVAGLGHASLGSVDW
                     PLLAQLLMGSLPGIWLGSRLVSRTPERLIRSILSLLLAWAGAKLVLI"
BASE COUNT          390 a          829 c          969 g          458 t
ORIGIN      
        1 gcacgtcgcc ctggtagaag gcggcaaaac gctcgaactg gcgctgggcg gcgctggcgt
       61 ccacgccctc ggcgagcacg gcgctggaga agcccgagcg gtgcatgtgc acgagctggt
      121 cgatcagcac atcgcccgtg gcgcggatgt cgcccgcaaa cttgtagcgc cggcgcagca
      181 gcagggcctg gctgtaggcg cggccgtcgg tgaacttggg gaattgcagc tcgatgcgct
      241 ccacgccctc gaacgcgccg ctggcggcca gctcgtccag gttggcgtcg ttggcaattt
      301 gcaaggtttt ttcggcttca gcgcttgtgg ggagcgcgtc gctagctagt attttcatag
      361 tcatcaccgg ggtggcttgt cgtttcagtg cagggcttcc tcggccttgg catggcgcgc
      421 ggcgttggcg gcctgcttga aggggtcgtg gcctatgcgg cgcagggcgt cgatgaagaa
      481 ctcgcccggc tggcgcagct cgcggtaggt gtccagaatg gcctcgatca cgccgggcac
      541 ctcggccgcg gtgaacgagg ggccgacgac cttgccgccg atcgccgggc ccgagagctg
      601 cgagccgtcg gcgccgccca gcgtgatctg gtaccactcc ttgccgtcct tgtcgacgcc
      661 gaggatgccg atatggccgc tgtggtggtg gccgcagctg ttgatgcagc cgctcatgtg
      721 caggtcgatg gggccgaggt cgaagagctc gtcgaggtcc tggtagcgct cggtcacggc
      781 ggccgcgatg tgcagcgagc gcgcgttggc cagtgagcag aagtccccgc ccgggcaggc
      841 gatcatgtcg gtcagcaggc caatgttggg ctgggccagg cccagtttgc gcgcggcctc
      901 gtagagcgcg ggcaggtcgc tctcgaacac ccagggcagc agcaggttct gctcgtgcgt
      961 caggcgtgcc tcgcccgcgc tgaattgctc ggccagctgg gccagggcgt ccatggtgtc
     1021 ggcgtcggcg tcgcccgggg caaagcccac gcgcttgaac gacagggtca cggtcttcat
     1081 gcccgcgagc ctgtggccgc gcacgttctg ctgcagccag cgccggtaga gctggccctg
     1141 ggggccgacc ttgctgggca ggttggcggg cttcaattcg ggcacgacga agtggctggc
     1201 cacgcggtcg agctcggcct gggtgatgcg gtggggtgcg ccgtcgagct cgacgatgtc
     1261 gcggtattcc ttctccaccg cgtcgatgaa gccctggccc tcgctcttca ccagaatctt
     1321 gatgcgcgcc ttccacttgt tgtcgcgccg gccgtagccg ttgtagacgc gcacgatggc
     1381 ctcgatgtag ttgagcagct ctgcccaggg caggaactcg cgcaccgtcg tgccgatgat
     1441 gggcgtgcgc cccatgccgc cgcctacctt gacggtgaaa cccacctcgc cggcctcgtt
     1501 cttgagcgcc tgcaggccga tgtcgtacca gccaatcgcg gcgcggtcct ccacggcgcc
     1561 gttgaaggcg atcttgaact tgcgcggcag gtagctgaac tcggggtgca gcgaactcca
     1621 ctggcgcgtg atctctgcaa acgggcggca atcgacgatg ccgtcctcgg caatgccctc
     1681 ccaggcgtcg caggtgatgt tgcggatgtc gttgccactg gtctgaatgc cgtgcatgtg
     1741 cacgctggcc agcaggtcca tcacgtcggc cgccttggtg atcggaatcc agttgaactg
     1801 gcagttggtg cgcgtggtga agtggccgta gccgtatttg agcggcttgg ccgccagtgt
     1861 ggcgccgggc tggctcgctt ccagggcatc ctgcgtcgcc tgggcgtggg ccagcagctc
     1921 tggcgtgggc tggtcgtatt cgcgtgcgat gcggcccagc gtgcgcagct gcaggctgct
     1981 gatctcgcca tagggcacgg cgatgcgggc catgggcgcg tagcgctgga tgtaccagcc
     2041 gttctgcagg cgcagcggca gcatctgctc gtcggtgagc tcgccgcgct gccagcgctc
     2101 cagttggtcg cggaactgct gggcgcgcag cttgacgaat tgcttgtcga agtcggtgta
     2161 ttggtacatg gcgtggggag ggagaaatca ggtattcaga tcaggaccag cttggcgccg
     2221 gcccaggcca gcagcaggga gaggatggag cggatcagcc gctcgggcgt gcggctgacc
     2281 aggcgcgagc ccagccagat gcccggaagc gaccccatga gcagctgcgc caagagcggc
     2341 caatcgaccg agcccagcga cgcgtggccc aggcccgcga ccagggtcag cggcacggcg
     2401 taggcaatgt cggcggcgat gatgcgcggc agcggcaggt gcgggaagac gagcagcagc
     2461 acgctcacac cgatcgcacc cgcgcccacc gaggtgaagg tgaccagcat gccgatgacc
     2521 gcgcccagca gcacgggcag gctccagtgg cgcggtgcgc tggtgtgggc gaactgggcg
     2581 ctgcggcgtg cggcctcggc ggccgcgcgc tcgggcgaga acaccagcac cttgtagaag
     2641 gtggcc
//
LOCUS       DAOOOG010000274         2644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_47655_length_2644_cov_26.480108, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000274 DAOOOG010000000
VERSION     DAOOOG010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47655_length_2644_cov_26.480108"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..621)
                     /gene="accB"
                     /locus_tag="PK242_13225"
     CDS             complement(151..621)
                     /gene="accB"
                     /locus_tag="PK242_13225"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOZ95026.1"
                     /translation="MDLRKLKTLIDLVSESNVSELEITEAEGKVRIVKSGQPAAAQVV
                     YAPVAAPAVAPAAPPAAAAPAAAAPQAVAEAAESTGFVVKSPMVGTFYRAASPGAKPF
                     VEVGQAVKEGDTLCIIEAMKILNEIEAEKSGTVTRIMGENGQAVEFGQPLFVIE"
     gene            complement(748..1320)
                     /locus_tag="PK242_13230"
     CDS             complement(748..1320)
                     /locus_tag="PK242_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOZ95027.1"
                     /translation="MTLPEPQPAPVVAPPARPLGRRLLVAGVALAAAGAGAWYAARRL
                     QLSEPQLAGFWERSFPTPDGSTLALAGLRGRPLLVNFWATWCPPCIAELPLLSAFYTE
                     NKANGWQVLGLAVDKPEPVGRFLAQTPVSFPVALAGLEGASLTRELGNSAGGLPFSVL
                     FDAQGTLRERKLGQLREGDLRAWRKAAGSA"
     gene            complement(1317..1913)
                     /locus_tag="PK242_13235"
     CDS             complement(1317..1913)
                     /locus_tag="PK242_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95028.1"
                     /translation="METLTQELAAHAARLVVEEGLDYGGAKRRAHKLMGLPARTALPD
                     NELVEAEVRAYLALFHADTQPAELAALRRLALRWMERLAEFRPHVTGAVWNGTATRRS
                     DVWLQLFCDDPKSAEISLIDHQVRYQARSINGLHGVPVDTLSFSSPCPELGEPIGVHL
                     MIHDHDELRGALKPGRLGQPLRGDRQALVQRMQMEPTP"
     gene            1985..>2644
                     /locus_tag="PK242_13240"
     CDS             1985..>2644
                     /locus_tag="PK242_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ95029.1"
                     /translation="MQATMMSTPLSLNHLLERAGRLFHDNTIVSRLPDKSLRRHSYGE
                     YYRRTRALAAALQGLGLQKGDRVATLCWNHHAHLECYFGIPAAGGVMHTLNLRLPPEE
                     IGWIAGDAADRFLIIDDVLLPLYQQFAHLHRFERVIVFAFSGAPVAPEFTDYEALLAA
                     ADPDAFDYAPHAEDDPTSMCYTSGTTGKPKGVVYSHRSTVLHSLVGALPDATAVRNTD
                     VV"
BASE COUNT          380 a          950 c          879 g          435 t
ORIGIN      
        1 ggcgaccagg attttcttaa acatcagcac cctcgcggct catcgcccgc acgctggcgc
       61 gtgccagcgc aatttgcttc gcctggcctg cggccagacc gccgcggttc gcgaccagaa
      121 ttttcttgaa catccgctgc ccgcagcttc ttattcgatg acgaacagcg gctggccgaa
      181 ctcaaccgcc tggccgttct cgcccatgat ccgggtgacg gtgccgcttt tttcggcctc
      241 gatctcgttg aggatcttca tcgcttcgat gatgcacagc gtgtcgccct ccttgaccgc
      301 ctgccccacc tcgacgaagg gcttggcgcc cgggctggcg gcgcggtaga aggtgccgac
      361 catgggcgac ttgacgacga agccggtgct ttcggccgct tcggccaccg cctgcggtgc
      421 cgcggcggcc ggcgcggcgg ctgccggggg cgccgcaggc gccacggccg gcgcggccac
      481 gggggcgtag accacctggg ccgccgccgg ctggccgctc ttgacgatgc gcaccttgcc
      541 ttcggcttcg gtgatttcca gttcagacac gttagactcc gacaccaggt cgatcagggt
      601 cttgagcttg cgcagatcca tgttgcttac caattcattg ggaacagggt cgaatttacc
      661 tcaattcgcg tcgatttgcg tcgaattgcc gctaattttc aattatctgg cttgcgcgcg
      721 gcatcacgcc aaggccgctg gcagccctca ggcgctgcct gcggccttgc gccaggcgcg
      781 cagatcgcct tcgcgcagtt gccccagctt gcgttcgcgc agcgtgccct gggcgtcgaa
      841 cagcacgctg aacggcaggc cgccggcact gttgcccagc tcacgcgtca ggctggcgcc
      901 ctccagcccc gccagcgcca ccggaaagct gaccggcgtc tgggccagga aacgccccac
      961 gggctccggc ttgtcgaccg ccaatccgag cacttgccag ccgttggcct tgttttcggt
     1021 gtagaaggcg ctcaatagcg gcagttcggc gatgcagggc gggcaccagg tggcccagaa
     1081 gttgaccagc agcgggcgcc cgcgcaggcc ggccagcgcc agcgtgctgc cgtcgggggt
     1141 ggggaagctg cgctcccaga aaccggccag ctggggttcg ctcagttgca ggcgacgcgc
     1201 cgcataccag gcgccggcgc cggccgccgc cagcgccacg ccggccacca gcaggcggcg
     1261 ccccagcgga cgggcgggcg gcgcaacgac cggcgctggc tggggttcgg ggagggtcat
     1321 ggcgtgggct ccatctgcat gcgctgcacc agcgcctgcc ggtcgccgcg cagcggctgg
     1381 cccaggcggc cgggcttgag cgcgccgcgc agctcgtcgt ggtcgtggat catcaggtgc
     1441 acgccgatcg gctcgcccag ctccgggcag gggctggaaa agctcagtgt atccaccggc
     1501 acgccgtgca ggccgttgat gctgcgcgcc tggtagcgca cctggtggtc gatgaggctg
     1561 atttcggccg acttgggatc gtcgcagaac agttgcaacc agacgtccga gcgccgcgtg
     1621 gcggtgccgt tccacaccgc cccggtgacg tgcggccgga attcggccag ccgctccatc
     1681 cagcgcaacg ccagccgccg cagcgcggcc agctcggccg gctgggtgtc ggcatgaaac
     1741 agcgccaggt aggcgcgcac ctcggcttcc accagctcgt tgtcgggcag ggcggtgcgc
     1801 gcgggcaggc ccatgagctt gtgggcgcgc cgcttggcgc cgccgtaatc cagcccctcc
     1861 tccaccacca ggcgggcggc gtgggcggcc aactcctgcg tcagggtttc catgggtgtc
     1921 attgtgccct tgcgggcgcc cggactcact tacattcgtt cgccaatcca ccggagagcc
     1981 gccaatgcaa gccaccatga tgtccacgcc gctgtcgctc aaccacctgt tggagcgcgc
     2041 cgggcggctg tttcacgaca acaccatcgt ctcgcgcctg cccgacaaga gcctgcggcg
     2101 ccacagctac ggcgagtact accgccgcac gcgggcgctg gccgcggcgc tgcagggcct
     2161 gggcctgcaa aagggcgacc gcgtggccac cctgtgctgg aaccaccacg cgcacctgga
     2221 gtgctatttc ggcatcccgg ccgccggcgg cgtgatgcac accctgaacc tgcgcctgcc
     2281 gccagaggag atcggctgga tcgccggcga cgcggcggac cgctttctga tcatcgacga
     2341 cgtgctgctg ccgctgtacc agcagttcgc ccatctgcac cgcttcgagc gggtgatcgt
     2401 gtttgccttc tccggcgcgc cggtggcgcc cgagttcacc gactacgagg cgctgctggc
     2461 ggccgccgac cccgacgcct tcgactacgc cccgcacgcc gaggacgacc cgacctccat
     2521 gtgttacacc tcgggcacca ccggcaagcc caagggcgtg gtgtattcgc accgctccac
     2581 ggtgctgcat tcgctggtcg gcgccctgcc cgacgccacc gccgtgcgca acaccgacgt
     2641 ggtg
//
LOCUS       DAOOOG010000275         2627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_48084_length_2627_cov_22.949067, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000275 DAOOOG010000000
VERSION     DAOOOG010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48084_length_2627_cov_22.949067"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1216
                     /locus_tag="PK242_13245"
     CDS             2..1216
                     /locus_tag="PK242_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOZ95030.1"
                     /translation="MTRPLDGITVISLEHAVAAPFATRQLADLGARVIKVERPGGGDF
                     ARGYDQRVRGQSSHFTWLNRNKQSLALDVKHPEARAALLQLLKTADVLVQNLAPGAAA
                     RMGLSYEALKGHNPRLIVCDISGYGANGPYRDKKAYDLLIQSEAGFLSVTGTPETPSK
                     SGISVADIAAGMYAYTNILSALLLRGKTGEGSHIDVSMLEAMGEWMGYPMYYAFDGAP
                     PPPRTGASHASIYPYGPFAAGDGGTVMLGLQNEREWQAFCEQVLRRPEVAVDARFCSN
                     AARNQHRDELQALILQEFATLTAAQVVERLDAAGIANARVNDMAGLWAHPQLAARERW
                     REVDTPAGRIPALLPPGVSSAFEYRMEQVPAVGQHNAAILAELGWTAERIAALAAAPA
                     ARETIKTIAVGA"
     gene            complement(1275..1913)
                     /gene="nth"
                     /locus_tag="PK242_13250"
     CDS             complement(1275..1913)
                     /gene="nth"
                     /locus_tag="PK242_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOZ95031.1"
                     /translation="MTPAQIDRFFTTLQAANPLPASELEYTSVFELLTAVLLSAQATD
                     VGVNKATRRLFPVANTPQQVLALGLERLESHIRTIGLYRSKARHLMQTCAILVEHHGG
                     EVPRTREALERLPGVGRKTANVVLNVAFGEPTMAVDTHIFRVGNRTGLAPGKTPLAVE
                     QQLLRRVPERFLPHAHHWLILHGRYVCLARKPRCWECAVADCCDFQPKTPAP"
     gene            1976..>2627
                     /locus_tag="PK242_13255"
     CDS             1976..>2627
                     /locus_tag="PK242_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOZ95032.1"
                     /translation="MSLPFAQRLEHVETSAIRELFKLLGKPGIISFAGGFPDSAMFDV
                     AGIGEAVNAALATEPGAALQYGATEGHPPLREQLAAFMRSKGVSELTPEQLIVTTGSQ
                     QALDLLGKCLVDPGSKAIVEGPTFLATIQCFRLYGADLVSAPIDGEGVQVDELERLIR
                     THRPKFVYLIPTFGNPSGAMLSLARRRRVLELAVQYQTLIIEDDPYGDLYFDAPPPP"
BASE COUNT          413 a          943 c          874 g          397 t
ORIGIN      
        1 catgacccgc cccctcgacg gcatcaccgt catctccctg gaacacgcgg tcgccgcgcc
       61 ctttgccacg cgccagctcg ccgacctggg cgcgcgcgtg atcaaggtcg agcgccccgg
      121 cggcggcgac ttcgcgcgcg gctacgacca gcgcgtgcgc ggccagtcgt cgcacttcac
      181 ctggctcaac cgcaacaagc aaagcctggc gctggacgtc aagcaccccg aggcccgcgc
      241 cgcgctcttg cagctcctga aaaccgccga cgtgctggtg cagaaccttg cgcccggcgc
      301 ggccgcgcgc atggggctgt cgtacgaggc gctcaaaggc cacaacccca ggctcatcgt
      361 ctgcgacatc agcggctatg gcgccaacgg gccgtaccgc gacaagaagg cctatgacct
      421 cttgatccag agcgaggcgg gttttctgtc cgtcaccggc acgcccgaga cgccgtccaa
      481 atccggcatc tcggtggccg acatcgccgc cggcatgtat gcctacacca acatcctgtc
      541 ggccctgctg ctgcgcggca agaccggcga gggcagccat atcgacgtct ccatgctcga
      601 ggccatgggc gagtggatgg gctatcccat gtactacgcc ttcgatggcg ccccaccacc
      661 gccgcgcacc ggcgcatcgc acgccagcat ctacccctac gggccgtttg ccgcgggtga
      721 tggcggcacg gtcatgctgg gcctgcagaa cgagcgcgaa tggcaggcct tttgcgagca
      781 ggtgctgcgg cgccccgagg tggccgtcga tgcacgcttt tgctccaacg ccgcgcgcaa
      841 ccagcaccgc gatgagctgc aggcgctgat cctgcaggag ttcgcgacgc tcaccgcagc
      901 ccaggtggtc gagcgcctgg atgcggcagg gattgccaat gcgcgagtga acgacatggc
      961 ggggctgtgg gcgcacccgc agcttgcagc acgcgagcgc tggcgcgagg tggacacccc
     1021 cgcgggccgc atcccggccc tgctgccgcc gggcgtcagc agcgcgttcg aataccgcat
     1081 ggaacaagtc cccgccgtgg gccagcacaa cgccgccatc ctcgccgagc tgggctggac
     1141 ggccgaacgg atcgccgcgc tggcggccgc cccggcggcc cgggaaacta tcaaaacgat
     1201 agctgttggc gcttgatgca agcgcgccag aggcgttttt tgcttatgaa tcggcgcggc
     1261 gccccggggc gccgtcacgg cgccggcgtc ttcggctgga agtcgcagca gtcggccacc
     1321 gcgcattccc agcagcgtgg cttgcgcgcc aggcagacgt agcggccgtg caggatcagc
     1381 cagtggtgcg cgtgcggcag aaagcgctcg ggcacgcggc gcagcagctg ctgctccacc
     1441 gccagcggcg ttttgcccgg cgccaggccg gtgcggttgc cgacgcggaa gatgtgcgtg
     1501 tcgaccgcca tcgtcggctc gccgaaggcc acgttcagca ccacgttggc ggtcttgcgg
     1561 ccgacgccgg gcaagcgctc cagcgcctcg cgcgtgcgcg gcacctcgcc gccgtggtgt
     1621 tccaccagaa tggcgcaggt ctgcatcagg tggcgcgcct tgctgcggta caggccgatg
     1681 gtcctgatgt ggctctccag ccgctccagc cccagcgcca gcacctgctg cggcgtgttg
     1741 gccaccggaa acagccgccg cgtggccttg ttcaccccca cgtcggtggc ctgcgccgac
     1801 agcagcaccg ccgtcagcag ctcgaacacg ctggtgtatt ccagctcgct ggccggcagc
     1861 gggttggcgg cctgcaaggt ggtgaagaag cggtcgatct gggccggggt catggcgccg
     1921 attatggcca gcgcgcgccg cgtcacaata aggctcccgt tccacaggtt gacccatgag
     1981 ccttccgttt gcccagcgcc tagagcacgt cgaaacctcc gccatccgcg agctgttcaa
     2041 gctgctgggc aagcccggca tcatcagctt tgccggcggc tttcccgaca gcgccatgtt
     2101 cgacgtggcc ggcatcggcg aagccgtgaa cgcagccctg gccaccgaac ccggcgcggc
     2161 cctgcagtac ggtgccaccg agggccatcc gccgctgcgc gaacagctgg ccgccttcat
     2221 gcgcagcaag ggcgtgtcgg agctgacgcc cgagcagctg atcgtcacca ccggcagcca
     2281 gcaggcgctg gatctgctgg gcaaatgcct ggtcgatccg ggcagcaagg ccatcgtgga
     2341 gggaccgacc tttctggcca ccatccagtg tttcaggctc tatggggccg atctggtcag
     2401 cgcgccgatc gacggcgagg gcgtgcaggt ggacgagctg gagcggctga tccgcacgca
     2461 ccggccgaag ttcgtctatc tgatccccac tttcggcaac cccagcggcg ccatgctcag
     2521 cctggcgcgc cgccgccgcg tgctggagct ggcggtgcag taccagacgc tcatcatcga
     2581 ggacgacccg tatggcgacc tgtacttcga cgcgccgccg ccgccca
//
LOCUS       DAOOOG010000276         2621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_48235_length_2621_cov_39.770460, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000276 DAOOOG010000000
VERSION     DAOOOG010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48235_length_2621_cov_39.770460"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PK242_13260"
     CDS             <1..859
                     /locus_tag="PK242_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOZ95033.1"
                     /translation="GCETTSMKMGSPEAKTVATGSAAGAATSGENTALEKCDSPLGTV
                     SLVENQTAGWYTILTGEYKLPPTANLLRLLVQQSNCFVVVERGAAGMRAMSRERELMQ
                     SGEMRGGSNFGKGQMVASDYALSPEIVFSADNAGGIGGVLGGLIGGGSGRAVAAIGGG
                     LQTREASAMLTLVDNRSGVQVAASEGSASKTDLNLFGGLFGGRAGAGLGGYTNTPQGK
                     VISAAFMDAFNQMVRSLRNYRAQSVQGQGLGGGGRLGVDGGAAPSQTYVPENAPKSAP
                     SKRAPTRRR"
     gene            1146..1772
                     /locus_tag="PK242_13265"
     CDS             1146..1772
                     /locus_tag="PK242_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOZ95034.1"
                     /translation="MSLLELIRRRRASLPDWVQEWLDVLVPALQVALIVLAAWLLHWL
                     LKRLVLRLARLYALPPAIVLPLRGVLRWLIIVATALLVLGRLGVSAEVLWTAFTGFAA
                     VAAVAFFAAWSVLSNLFCALLIMVAQPFRLGDDLEVLDSAEKPGAKGRVVDINLLYTT
                     LQETDGGGRLLQIPNALFFQRVVRRWRGGMAAAAADPSAAAPAGHEPG"
     gene            complement(1808..>2621)
                     /locus_tag="PK242_13270"
     CDS             complement(1808..>2621)
                     /locus_tag="PK242_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOZ95035.1"
                     /translation="DLTRLFVGSEGTLGVITEITLRLYPLPEAVSAAICSFPSIAEAV
                     GTVIQIIQLGVPIARVELLDARTVRAVNQHSKLSLREAPMLLMEFHGSPAGVREQAET
                     VQAIAGEHGGAGFEWADTPEERTRLWTARHKAYFAGVQSRPGCRCITTDVCVPISRLA
                     DAVLASVQEADASGIPYFLVGHVGDGNFHMGYLIDPDDDAERARAEQLNHQVVSRALA
                     LGGTCTGEHGVGLHKMGFLLDEAGAGAVALMRTIKQALDPHNILNPGKVLAP"
BASE COUNT          363 a          894 c          967 g          397 t
ORIGIN      
        1 cggctgcgaa accaccagca tgaagatggg cagcccggag gccaagaccg tggccaccgg
       61 cagcgccgcc ggcgccgcca cctcgggcga gaacacggcg ctggaaaagt gtgactcgcc
      121 gctgggcacg gtgtcgctgg tggaaaacca gaccgccggc tggtacacca tcctgaccgg
      181 tgaatacaag ctgccgccca ccgccaacct gctgcgcctg ctggtgcagc agtccaactg
      241 cttcgtggtg gtcgagcgcg gcgccgccgg catgcgcgcc atgagccgcg agcgcgaact
      301 gatgcagtcg ggcgagatgc gcggcggcag caatttcggc aagggccaga tggtggcctc
      361 cgactacgcg ctgtcgcccg agatcgtgtt cagcgccgac aacgccggcg gcatcggcgg
      421 cgtgctgggc ggcctgatcg gtggcggcag cggccgcgcc gtggcggcca tcggtggcgg
      481 cctgcagacg cgtgaggcca gcgccatgct gaccctggtg gacaaccgct ccggcgtgca
      541 ggtggcggcg tcggaaggca gcgcctccaa gaccgacctc aacctgttcg gcggcctgtt
      601 cggcggccgt gccggtgccg gcctgggcgg ctacaccaac acgccgcagg gcaaggtcat
      661 cagcgccgcc ttcatggacg ccttcaacca gatggtgcgc agcctgcgca actaccgcgc
      721 gcaaagcgtg caaggccagg gcctgggcgg cggcggtcgc ctgggtgtgg acggcggggc
      781 cgcgcccagc cagacctacg tacccgaaaa cgcacccaag agcgcgccca gcaagcgcgc
      841 accgacgcgc cggcgctgag cgccggtcac accggcatgg ccgggtgcgg gtggcgcggg
      901 gcacccggac gaacccgcgc atgctacgat aatcatagct gccggcgctt gccatacggg
      961 cgctggtggc ttaaggttcg attgcccgat gcgggctcgc tttcagatgg gccaatggcg
     1021 gatgacctgg cggcgcttcg atcaccaagc ggtcatcggt ctggggccat gtctctgaac
     1081 gcgaagccgt gtgaacccgt ggcgcgcagg ccgggcccgc cgccgcgcgc tgccgcccgt
     1141 tgcccatgag cctgcttgag ttgatccgcc gccgccgcgc cagcctgccc gactgggtgc
     1201 aggaatggct ggacgtgctg gtgccggcgc tgcaggtggc gctgatcgtg ctggccgcct
     1261 ggctgctgca ctggctgctc aagcgcctgg tgctgcgcct ggcgcgcctg tacgccctgc
     1321 cgccggccat cgtgctgccg ctgcgcgggg tgctgcgctg gctgatcatc gtcgccacgg
     1381 cgctgctggt gctgggccgg ctgggggtgt cggccgaggt gctgtggacc gccttcaccg
     1441 gctttgccgc ggtggcggcg gtggccttct ttgccgcctg gagcgtgctg tccaacctgt
     1501 tctgcgcgct gctcatcatg gtggcgcagc cgtttcggct gggcgacgac ctggaggtgc
     1561 tggacagcgc cgaaaaaccc ggcgccaagg gccgtgtggt ggacatcaac ctgctctaca
     1621 ccacgttgca ggaaaccgat ggcggcgggc ggctgctgca gattcccaac gcgctgttct
     1681 ttcagcgcgt ggtgcggcgc tggcgcggtg gcatggcggc cgccgccgcc gacccgtcgg
     1741 cagcagcgcc ggccggtcac gaaccaggct gagcgccggc cggtcacgaa ccaggctgag
     1801 cgccggccta cggtgccagc accttgcccg ggttcaggat gttgtgcggg tccagcgcct
     1861 gcttgatggt gcgcatcagc gccaccgccc cggcgccggc ctcgtcgagc agaaagccca
     1921 tcttgtgcag gcccacgccg tgctcgccgg tgcaggtgcc gcccagggcc agcgcgcggc
     1981 tgacgacctg gtggttgagc tgttcggcgc gggcgcgctc ggcgtcgtcg tcggggtcga
     2041 tcaggtagcc catgtggaag ttgccgtcgc ccacgtggcc gaccaggaag taggggatgc
     2101 cgctggcgtc ggcttcctgc acgctggcca gcacggcgtc ggccaggcgc gagatcggca
     2161 cgcagacgtc ggtggtgatg cagcggcagc cggggcggct ttgtacgccg gcgaaatagg
     2221 ccttgtgccg cgccgtccac aggcgggtgc gctcctcggg cgtgtcggcc cactcgaagc
     2281 cggcgccgcc gtgctcgccc gcgatggcct gcaccgtttc ggcctgctcg cgcacgccgg
     2341 cgggcgagcc gtgaaactcc atcagcagca tcggtgcctc gcgcaggctg agcttgctgt
     2401 gctggttgac ggcgcgcacg gtgcgcgcgt ccagcagttc gacgcgggcg atcggcacac
     2461 ccagctggat gatctggatc acggtgccca ccgcttcggc gatgctgggg aaggaacaga
     2521 tcgccgccga caccgcctcg ggcagcgggt acaggcgcag ggtgatttcg gtgatgacgc
     2581 ccagcgtgcc ttcgctgccg acgaacaggc gcgtcaggtc g
//
LOCUS       DAOOOG010000277         2618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_48309_length_2618_cov_29.614514, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000277 DAOOOG010000000
VERSION     DAOOOG010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48309_length_2618_cov_29.614514"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PK242_13275"
     CDS             <1..820
                     /locus_tag="PK242_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ95036.1"
                     /translation="VAPSRLTRMLGFWGAFMPFGTALALLVGPGVMAALGWPGWWWLL
                     GLLSALMVPWVALTVPADAPHAQAPSAGASWGARLRQTLGSGGPWLAALSFGAYSAQW
                     LAVIGFLPSLYAQSGWTGALGAVLTALVAAVNIVGNVAAGRLLSHGVAPQRLLWCGFA
                     AMALGTFLAFGSATADAPLARYLGALLFSTVGGLIPGSLFALAPRLAPGEGTVSTTVG
                     WMMQWSAIGQFAGPPVAAWVAAQAGGWHWTWAVTGACCAVGALLAWRMGRRTKV"
     gene            830..1405
                     /locus_tag="PK242_13280"
     CDS             830..1405
                     /locus_tag="PK242_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HOZ95037.1"
                     /translation="MNMHDHAIADTRRWLERAVIGLNLCPFAKAPHIKGQIHYAVCVG
                     GGRRELLALLRAELLALAALDAAERETTLLIVPDQFADFLDFNDFLGDAEALLQALDL
                     EGVLQVAPFHPRFQFDGTDEDDITNATNRSPYPTLHLIREDSIDRAVQAFPEAEAIYE
                     ANMQTLRRLGPEGWAALDVGPSADADGRPAP"
     gene            1402..2388
                     /locus_tag="PK242_13285"
     CDS             1402..2388
                     /locus_tag="PK242_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOZ95038.1"
                     /translation="MSQDATKKRAAGAHHAGAAGQKHAEDAVRAELRPGQSIELLKAL
                     HILTREGRLNQDSRRKLKQVEHLVRFIEKILDELTPHGDQLTLADHGAGKSYLGFILY
                     DLWFKPRGSGCIWGIETRPELVERSRELAARLGFERMRFLNIAAADAAHEAALPARID
                     VVTALHACDSATDDAIAFGLAKQAQAMVLVPCCQAELAARLRMNKALQLSRTPLAELW
                     RHPLHTRELGSQLTNVLRCLYLESQGYGVTVTELVGWEHSLKNELIIARYSGHKKRSA
                     AERLRALLDEFGLGALLPLRFAALADTPQRPAVPAPDPEAPCIPAQKREAHD"
     gene            2381..>2618
                     /locus_tag="PK242_13290"
     CDS             2381..>2618
                     /locus_tag="PK242_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95039.1"
                     /translation="MTDIHDTQALTEGDRLFDQMFTRLMRGDSLQEFGDWFASAAAAP
                     GGPMDDPRAARAMARALWGAVPLPSNHWRARGLPK"
BASE COUNT          355 a          925 c          942 g          396 t
ORIGIN      
        1 cgtggcgccg tcgcggctga cgcgcatgct gggcttctgg ggcgccttca tgcccttcgg
       61 cacggcgctg gcgctgctgg tcggcccggg tgtgatggcg gcgctgggct ggcccggctg
      121 gtggtggctg ctgggcctgc tgtcggcact gatggtgccg tgggtggcgc tcaccgtgcc
      181 ggccgatgca ccgcacgccc aggcgccaag cgccggtgct tcctggggcg cgcggctgcg
      241 gcagacgctg ggcagcggcg ggccatggct ggcggcgctc agtttcggcg cctattcggc
      301 ccagtggctg gcggtgatcg gctttctgcc ctcgctgtac gcgcagtccg gctggacggg
      361 cgccctgggc gccgtgctga cggcgctggt ggcggcggtc aacatcgtcg gcaacgtggc
      421 ggccgggcgc ctgctgtcgc acggcgtggc gccgcagcgg ctgctgtggt gcggttttgc
      481 cgccatggcg ctgggcacct ttctggcctt cggcagcgcc accgccgacg cgccgctggc
      541 gcgctacctg ggggcgctgc tgttttccac cgtcggcggg ctgatccccg gcagcctgtt
      601 cgccctggcg ccgcgcctgg cccccggcga gggcaccgtc tcgaccaccg tcggctggat
      661 gatgcaatgg tcggccatcg gccagttcgc cggcccgccg gtggccgcct gggtggccgc
      721 gcaggcgggc ggctggcatt ggacctgggc cgtcaccggc gcctgctgcg ccgtcggcgc
      781 gctgctggcc tggcgcatgg ggcggcgcac gaaggtttag catgcgccca tgaacatgca
      841 cgatcacgcc attgccgaca cccgccgctg gctggagcgc gccgtcatcg ggttgaacct
      901 gtgcccgttc gccaaggcgc cgcacatcaa gggccagatc cactacgcgg tctgtgtggg
      961 cggcgggcgg cgcgagttgc tggcgctgct gcgcgccgag ttgctggcgc tggcggcgct
     1021 ggacgcggcc gagcgcgaga ccacgctgct gatcgtgccc gatcagtttg ccgactttct
     1081 ggacttcaac gactttctgg gcgatgccga ggcgctgctg caggcgctgg acctggaagg
     1141 cgtgctgcag gtggcgccgt ttcacccgcg ctttcagttc gacggtaccg acgaggacga
     1201 catcaccaac gccaccaacc gctcgccgta ccccacgctg cacctgatcc gcgaagacag
     1261 catcgaccgc gcggtgcagg cgtttcccga ggccgaagcg atttacgagg ccaacatgca
     1321 gacgctgcgc cggctcgggc ccgaaggctg ggcggcgctg gacgtggggc cctcggccga
     1381 cgccgacggc aggcctgccc catgagccag gatgctacaa aaaagcgagc tgctggcgct
     1441 caccatgcgg gcgctgcagg gcaaaaacac gctgaagatg cggtgcgcgc cgagctgcgc
     1501 ccggggcagt cgatcgagct gctcaaggcg ctgcacatcc tgacgcgcga ggggcggctg
     1561 aaccaggact cgcgccgcaa gctcaagcag gtcgagcacc tggtgcgctt catcgaaaaa
     1621 atcctggacg agctcacgcc ccacggcgat caactgacgc tggccgacca cggcgccggc
     1681 aagtcgtatc tgggcttcat cctgtacgac ctgtggttca agccacgcgg cagcggctgc
     1741 atctggggca tcgaaacccg acccgagctg gtcgagcgct cgcgcgaact ggcggcgcgg
     1801 ctgggctttg agcgcatgcg ctttctcaac atcgccgccg ccgacgcggc gcacgaggcc
     1861 gcgttgccgg cgcgcatcga cgtggtcacc gcgctgcacg cctgcgacag cgccaccgac
     1921 gacgccatcg ccttcggcct ggccaagcag gcgcaggcca tggtgctggt gccctgctgc
     1981 caggccgagc tggccgcgcg cctgcgcatg aacaaggccc tgcagctgtc gcgcacgccg
     2041 ctggccgagc tgtggcgcca cccgctgcac acgcgcgagc tgggcagcca gctgaccaac
     2101 gtgctgcgct gcctgtacct ggaatcgcag ggctacggcg tcaccgtcac cgagctggtg
     2161 ggctgggaac acagcctgaa gaacgagctg atcatcgccc gctacagcgg ccacaaaaag
     2221 cgcagcgccg ccgagcgcct gcgcgcgctg ctggacgaat ttggccttgg cgcgctgctg
     2281 ccgctgcgct ttgcggcgct ggcggacacg ccgcagaggc cggccgtacc agcgcccgac
     2341 cccgaggcac cttgcatccc ggcccagaag cgagaggctc atgactgaca tccacgatac
     2401 ccaggcgctg accgaaggcg accgcctgtt cgaccagatg ttcacccgcc tgatgcgcgg
     2461 tgacagcctg caggagtttg gcgactggtt cgccagtgcg gcggcggcgc ccggcggtcc
     2521 gatggatgac ccgcgcgccg cccgcgccat ggcccgcgcg ctctggggcg cggtgccgct
     2581 gccctcgaac cactggcgcg cgcgcggcct gcccaaga
//
LOCUS       DAOOOG010000278         2608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_48550_length_2608_cov_26.531140, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000278 DAOOOG010000000
VERSION     DAOOOG010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48550_length_2608_cov_26.531140"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="PK242_13295"
     CDS             <1..128
                     /locus_tag="PK242_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOZ95040.1"
                     /translation="IGKLCARLMQLPGVQGVRVKITKLEIFDDCEVSIHMEAGHW"
     gene            186..1109
                     /gene="ttcA"
                     /locus_tag="PK242_13300"
     CDS             186..1109
                     /gene="ttcA"
                     /locus_tag="PK242_13300"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HOZ95041.1"
                     /translation="MIEEPVVADTGPAARTRAEREALKLEKRLARQVGQAIADFHMIE
                     AGDRVMVCLSGGKDSYGLLDILLKLKARAPIHFDLVAVNLDQKQPGFPAEVLPNYLSG
                     LGIEHHIETQDTYSIVKAKIPEGKTTCSLCSRLRRGILYRVARQLGCNKIALGHHRDD
                     MLQTLFLNMFFGGKLKGMPPKLVSDNGEFIVIRPLAYVAESDLVAWAEHRQFPIIPCN
                     LCGSQENLQRQQVGEMLREWQRRHPGRIESMFTALQNVVPSHLMDARLHDFKHLRPSG
                     VPDEHGDKAFDDEVFVPPVGAPFSSVTRVSA"
     gene            1141..1785
                     /locus_tag="PK242_13305"
     CDS             1141..1785
                     /locus_tag="PK242_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95042.1"
                     /translation="MWIARAALVLIASALTACASGPRVVDAHVRTHAAQPPGAAVLQG
                     AHYRFEPGPQRAGQPAPARLEAMAQAALTRVGAVRDEGGARVSVQVSGNVNALWPDPW
                     YEPSNARIALGLGHAWRGGAVGLGWGWPLGDPSVPTYASEVSLLMRDLQTGQIVYDTR
                     ARHHGPWHDTDAVLAALFVAALEGYPQPAASERRVGVPLLPPATPAAAPAATGR"
     gene            complement(1833..>2608)
                     /locus_tag="PK242_13310"
     CDS             complement(1833..>2608)
                     /locus_tag="PK242_13310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ95043.1"
                     /translation="TGSPLAWLIEATSWRMGFWVLAGCALLSWLAIALGVREPAPATG
                     ARRETLGQALRTFGALLRQRHTAGIVALALVSYGAFITLRGLWLGPLMTQRHGWSLVA
                     AGHLAVLLTVVSLVSLPLFGRADPGPRRRRRWMLWGSAVAALCFAGLAANGGMWLDVA
                     LMLVFMMLSGYGTLQYPDVKDAYEPAVIGRAMALFTMALFMGVALMQWATGAIAQAAQ
                     ARGLDPFGAVFGAIAVLLALGALAFRLLPQPASVRTDVE"
BASE COUNT          428 a          973 c          877 g          330 t
ORIGIN      
        1 tgatcggcaa gctgtgcgcg cgcctgatgc agctgcccgg cgtgcagggc gtgcgcgtga
       61 agatcaccaa gctggagatc ttcgacgact gcgaggtgtc catccacatg gaggccgggc
      121 actggtaagc cccggccgcc caccggcgcc gcgcagggcc gaccccgccg taggacaatc
      181 gcagcatgat cgaagaaccc gttgtggccg acaccggccc ggccgcccgc acgcgcgccg
      241 agcgcgaggc gctcaagctc gaaaaacgcc tggcccgcca ggtcggtcag gcgattgccg
      301 atttccacat gatcgaggcc ggcgaccgcg tgatggtgtg cctgtcgggc ggcaaggaca
      361 gctacggcct gctcgacatc ctgctcaagc tcaaggcgcg cgcgccgatt cacttcgatc
      421 tggtggccgt caacctggat cagaagcagc ccggctttcc ggccgaggtg ctgccgaact
      481 acctgtccgg cctgggcatc gagcaccata tcgaaacgca ggacacctac agcatcgtca
      541 aagccaagat ccccgagggc aagaccacct gcagcctgtg ttcgcgcctg cgccgcggca
      601 tcctgtaccg ggtggcgcgc cagctcggct gcaacaagat cgcgctgggc caccaccgcg
      661 acgacatgct gcagaccttg tttctcaaca tgttcttcgg cggcaagctc aagggcatgc
      721 cgcccaagct ggtcagcgac aacggcgaat tcatcgtcat ccgcccgctg gcctacgtgg
      781 cggaaagcga cctggtggcc tgggccgaac accggcagtt ccccatcatc ccctgcaacc
      841 tgtgcggcag ccaggaaaac ctgcagcgcc agcaggtggg cgagatgctg cgcgaatggc
      901 agcgccgcca cccggggcgc atcgagagca tgttcaccgc gctgcagaac gtggtgccct
      961 cgcacctgat ggacgctcgc ctgcacgact tcaagcacct gcgccccagc ggcgtgcccg
     1021 acgagcacgg cgacaaggcc ttcgacgacg aggtgtttgt tccgccggtg ggtgcgccgt
     1081 tttccagcgt gacgcgggtc agcgcctgag gtttcaacgc catatcagag gttagcgccc
     1141 atgtggattg cgcgagcagc tcttgtttta atagcatctg cgctgaccgc ctgcgccagc
     1201 ggcccgcgcg tggtggacgc ccatgtgcgc acccacgccg cccagccacc cggcgccgcc
     1261 gtgctgcagg gggcgcacta ccgcttcgag cctggcccgc agcgggcggg ccagccggcg
     1321 ccggcgcggc tggaggccat ggcccaggcc gcgctgaccc gcgtgggcgc ggtgcgcgac
     1381 gagggcggtg cgcgcgtcag cgtgcaggtc agcggcaacg tcaacgccct gtggcccgac
     1441 ccctggtacg aacccagcaa cgcccgcatc gcgctcggcc tgggccatgc ctggcgcggt
     1501 ggcgccgtcg gtctgggctg gggctggccg ttgggggacc cgagcgtgcc cacctacgcg
     1561 agcgaagtca gcctgctgat gcgcgatctg cagaccggcc agatcgtcta cgacacccgg
     1621 gcccgccacc acggcccctg gcacgacacc gacgcggtgc tggcggcgct gttcgtggcg
     1681 gcgctcgaag gctacccgca gccggccgcc agcgaacgcc gcgtcggcgt gcccctgctg
     1741 ccgccggcca ccccggccgc cgcccccgca gccaccgggc gctgagacgg cctcggggct
     1801 cgatcagcgc ttgccgtcaa agcgcagcga ggtcattcga catccgtccg cacgctggcc
     1861 ggctgcggca gcaggcgaaa cgccagcgcg cccagcgcca gcagcacggc gatggcgccg
     1921 aacaccgcgc cgaacgggtc caggccgcgc gcctgcgccg cctgcgcgat ggcgccggtg
     1981 gcccactgca tcagcgccac gcccatgaac agcgccatgg tgaacagcgc catggcgcgg
     2041 ccgatcacgg ccggctcgta agcgtccttg acgtcggggt actgcaaggt gccgtagccc
     2101 gacagcatca tgaacaccag catcagcgcc acgtccagcc acatcccgcc gttggcggcc
     2161 aggccggcaa agcacagcgc ggcgacggcg ctgccccaca gcatccagcg ccggcgccgg
     2221 cgcggccccg ggtcggcacg gccgaacagc ggcaggctga ccagcgacac caccgtcagc
     2281 agcaccgcca ggtggccggc cgccaccagg ctccagccgt ggcgctgcgt catcagcggt
     2341 cccagccaca gcccgcgcag cgtgatgaag gcgccatagc tcaccagcgc cagcgccacg
     2401 atgccggccg tatggcgctg gcgcagcagg gcgccgaagg tgcgcagcgc ctggcccagg
     2461 gtttcgcgcc gtgcgcccgt cgccggcgcc ggctcgcgca cgcccagggc aatcgccagc
     2521 cacgacagca gcgcgcagcc ggccagcacc caaaagccca tgcgccagga cgtggcctcg
     2581 atcagccagg ccagcgggct gccggtca
//
LOCUS       DAOOOG010000279         2575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_49462_length_2575_cov_42.778175, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000279 DAOOOG010000000
VERSION     DAOOOG010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49462_length_2575_cov_42.778175"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PK242_13315"
     CDS             <1..318
                     /locus_tag="PK242_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOZ95044.1"
                     /translation="WVLSFQSRFGKAKWVEPATQTVLEQLGRAGTSRVDVICPGFVAD
                     CLETLEEIAIEGRAAFQAAGGQEFHYVPCLNDEPAWLAALAELVQDHLQGWPTRAPSS
                     SAK"
     gene            550..1104
                     /locus_tag="PK242_13320"
     CDS             550..1104
                     /locus_tag="PK242_13320"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOZ95045.1"
                     /translation="MPRRITGWITRGFELLIVACLTLMGILVFGNVVLRYAFNTGIAV
                     SEELSRLLFVWLIFMGAVLASAQRIHIGFDTLLHVVGPRVRRLLLLFTGLLLLAGCVI
                     FIVGGWRQTQINLANSYPVLGISYAWLYGVGLVFGVALLFPVCSNLLRAWRGEEGELT
                     EDLGAQIEAEARRVAAEADAEPRP"
     gene            1101..2378
                     /locus_tag="PK242_13325"
     CDS             1101..2378
                     /locus_tag="PK242_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOZ95046.1"
                     /translation="MTAAVFLLSLLGLIALGMPIAFALMFSGVALMLVMGQFDAQIVV
                     QNAISGADNFVLMAVPFFILAGEFMNAGGLSRRIVDMASAFVGHLRGGLGYVAIFAGI
                     LLASLSGSAVADTAALAAILVPMMRRAGYDPGRACGLMAASGIIAPVIPPSIGFLIFG
                     VAANVSITRLFLAGIVPGVMMGLVLVVAWWIVARRQQVSVTPRVPWGRRLRLLAQGGW
                     ALMLPVIIIGGLRFGIFTPTESAVVAAVYALFVGVVVYRELSARRIYQCLLVTARTTS
                     VVLLLAALAMVASYMITIADISGQVGAWLQTISDRPLVLMTAMMVVVFFAGMVLDFIP
                     IVLIFTPVFMPIVKAADIDPVYFGVVFIMNCSIGLITPPVGVVLNVVSSVGKVSMAEA
                     VRGIAPFLLALLALLALLILFPALVTVPAEWWR"
BASE COUNT          333 a          826 c          904 g          512 t
ORIGIN      
        1 tgggtgctga gctttcagtc gcgctttggc aaggccaagt gggtggagcc ggccacccag
       61 accgtgctgg agcaattggg ccgcgccggc acgtcgcgcg tggacgtgat ctgcccggga
      121 ttcgtggccg attgcctgga gacgctggaa gagattgcca tcgaaggccg cgccgcgttc
      181 caggccgccg gggggcagga gtttcattac gtgccctgcc tgaacgacga accggcgtgg
      241 ctggccgcgc tggccgaact ggtccaggac cacttgcaag gctggccgac gcgggccccg
      301 tcgagctcgg cgaagtgata tcaaaatgat agctgctggc gcttgactgt aaagcgccgg
      361 agagcttttt ctttgttgat ttcactgcgt tcgcaggagc acccgcaggg ggtgcgaggc
      421 cgctgcccgg acggggcgct gaccggggaa acaaccgatg gagccgccgc atcagatcgg
      481 gttcaatgca ggccatctgt ccaaccgagg aacccgtcat gcaccaccac cgtcccagga
      541 ccgcgcgcca tgcctaggcg catcaccggc tggatcacgc gcggcttcga actgctgatc
      601 gtcgcctgcc tgaccctgat ggggatcctg gtgttcggca acgtcgtgct gcgctacgcc
      661 ttcaacaccg gcatcgccgt ctctgaggag ttgtcgcgcc tgctgttcgt gtggctgatc
      721 ttcatgggcg cggtgctggc ctcggcgcag cgcatccaca tcggcttcga caccctgctg
      781 cacgtggtgg gcccgcgcgt gcggcgcctg ctgctgctgt tcaccgggct gctgttgctg
      841 gccggctgcg tgatcttcat cgtcggcggc tggcgccaga cgcagatcaa cctggccaac
      901 agctacccgg tgctgggcat ctcctacgcc tggctgtacg gcgtgggcct ggtgttcggg
      961 gtggcgctgc tgtttccggt ttgcagcaac ctgctgcgcg cctggcgcgg tgaggagggc
     1021 gagctgaccg aggacctggg cgcgcagatc gaggccgagg ccaggcgcgt ggccgccgaa
     1081 gccgacgcgg agccgcgccc gtgaccgccg cggtgtttct gctgtcgctg ctggggctga
     1141 tcgcgctcgg catgccgatc gcctttgcgc tgatgttctc aggcgtggcg ctgatgctgg
     1201 tgatgggcca gttcgacgcc cagatcgtgg tgcagaacgc catctccggc gccgacaatt
     1261 tcgtgctgat ggcggtgccg ttcttcatcc tggccggcga gttcatgaac gccggcggcc
     1321 tgtcgcggcg catcgtcgac atggccagcg ccttcgtcgg tcatctgcgt ggcggcctgg
     1381 gctatgtggc catctttgcc ggcatcctgc tggccagcct gtccggttcg gcggtggccg
     1441 acacggccgc gctggcggcc attctggtgc ccatgatgcg gcgcgccggc tacgacccgg
     1501 gccgcgcctg cggcctgatg gcggccagcg gcatcatcgc gccggtgatc ccgccctcga
     1561 tcgggttcct gatcttcggc gtggcggcca acgtgtcgat cacgcggctg ttcctggccg
     1621 gcatcgttcc gggcgtgatg atgggcctgg tgctggtggt ggcctggtgg atcgtggcgc
     1681 gccgccagca ggtcagcgtc acgccacgcg tgccctgggg ccggcggttg cgcctgctgg
     1741 cacagggcgg ctgggcgctg atgctgccgg tgatcatcat cggcggtctg cgattcggca
     1801 tcttcacgcc caccgagtcg gcggtggtgg cggcggtgta cgcgctgttc gtcggtgtgg
     1861 tggtgtaccg cgagctgagc gcgcgcagga tctaccaatg cctgctggtc accgcgcgca
     1921 ccacttcggt ggtgctgctg ctggcggcgc tggccatggt ggcgtcgtac atgatcacca
     1981 tcgccgacat ctcgggtcag gtcggcgcct ggctgcagac catctccgac cggccgctgg
     2041 tgctgatgac cgccatgatg gtggtggtgt tcttcgccgg catggtgctg gacttcattc
     2101 ccatcgtgtt gatcttcacc ccggtgttca tgcccatcgt caaggcggcc gacatcgacc
     2161 cggtgtattt cggtgtcgtg ttcatcatga actgctcgat cggcctgatc acgccgccgg
     2221 tgggcgtggt gctgaacgtg gtcagctcgg tcggcaaggt gagcatggcc gaggcggtgc
     2281 gtggcattgc gccgtttctg ctggccctgc tggcgctgct ggcgctgctg atcctgtttc
     2341 cggccctggt caccgtgcca gccgagtggt ggcgctgagc cgcacactgt ccgatttcat
     2401 ccaaggagac tcccatgaaa ctgcgcattt ctctcgtcgc cggcctgctg gccgccgccc
     2461 tgaccggccc cgcctgggcg gcggtcaccg gcaagttcgc cgtcaccctg tcggaaaaga
     2521 cccaccaggg ccagggcgtg gccaagttcg tcgaactggt caaggccaag agcaa
//
LOCUS       DAOOOG010000280         2535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_50579_length_2535_cov_24.732661, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000280 DAOOOG010000000
VERSION     DAOOOG010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50579_length_2535_cov_24.732661"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PK242_13330"
     CDS             <1..503
                     /locus_tag="PK242_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOZ95047.1"
                     /translation="EVPVICAVNGPAIGAGCDLTTMCDIRIASEQATFAESFIRVGLL
                     PGDGGAWLLPRVVGKAVAAELTFTGRTVDAREALAIGLVSRVVAHEQLLAEARALAEQ
                     IAAQPGVTLRMAKKLLRAGERLDFAPLLEMSAGLQALAHKTPHHREAVDAFVQKRPPD
                     FTPFQD"
     gene            519..1382
                     /locus_tag="PK242_13335"
     CDS             519..1382
                     /locus_tag="PK242_13335"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOZ95048.1"
                     /translation="MIRSLFFAPANRPELVLKFPRFGADCCVIDLEDGTPPGEKRSAR
                     EALAQTVAQVRAAGLGGTLMVRVNVPDSEHYLADLEAAFASDIDGVVVPKVETTEQVF
                     PVLHWIRQRDAQAPRAQPRALIGGIESVRGVLNAVALCRDIDALSAVFFGAEDFAADI
                     GGRRTPRGDEVYTARSMVVMAARAAGRIAIDQAVIDIRNDALFLDDAGRGRDLGYEGK
                     ICVTPGQVKLAHRAFSPTPEERNYAQRLVETYEAAMARGLGTIDFEGRMIDGPTLKRA
                     QQVLALPVQEA"
     gene            1382..>2535
                     /locus_tag="PK242_13340"
     CDS             1382..>2535
                     /locus_tag="PK242_13340"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ95049.1"
                     /translation="MQLESLDSLDLARHVRPGDGVIFQQGCGEALSVAERFVQQRASY
                     SGARLFLGSGFSRSFQPEHADHLRFSGIGGIGSLRKLASAGALDPIPCHISSIEPLLR
                     QGVIASDVVMLQVSPPNERGEYSLGLVNDYVRTAIGRARVVIAEINRQIPWVHCDLPL
                     TAADISVAVETDRAPLELPPAPFGALEQRIAQHIGDLIGDRSTLQMGIGSVPEAIMSS
                     LADRRELGIHSGMIGDSAVTLMECGAVTNAHKGMDAGVTVSGVLFGTQKLYRYAERNP
                     QIRLHPTSYTHHIGVLARLKNFVSINSALEVDLTGQINAEAIGREHIGAVGGQVDYIR
                     AAALSEGGVSIVALPATGKGGDSKIVSRLSGPVTTARSDVDVVATEHGVA"
BASE COUNT          382 a          890 c          876 g          387 t
ORIGIN      
        1 tggaggtgcc ggtgatctgc gccgtcaacg gcccggccat cggcgccggc tgcgatctga
       61 cgacgatgtg tgacatccgc atcgcctcgg agcaggccac cttcgccgaa agctttattc
      121 gcgtcgggct gttgcccggc gacggcggcg cctggctctt gccccgggtg gtgggcaagg
      181 cggtggctgc cgagctgacc ttcaccggcc gcacggtgga tgcgcgcgag gcgctggcca
      241 tcggcctggt ctcgcgcgtg gtcgcgcatg agcagttgct ggccgaggcc cgcgccctgg
      301 ccgagcagat cgccgcccaa cccggcgtga cgctgcgcat ggccaagaag ctgctgcgcg
      361 cgggcgagcg gctcgatttc gcccccttgc ttgagatgtc ggccgggctg caggcactgg
      421 cgcacaagac gccccaccac agggaggcgg tcgatgcctt cgtgcaaaag cgcccacccg
      481 acttcacacc ctttcaggac tgaccccagc cagacaccat gatccgatcc ctgttctttg
      541 cccccgccaa ccggcccgag ctggtgctca agttcccgcg ctttggcgcc gactgctgcg
      601 tcatcgacct ggaagacggc acgcccccgg gcgaaaagcg cagcgcccgc gaggcgctgg
      661 cgcagaccgt ggcccaggtg cgcgccgccg gcctgggcgg cacactgatg gtgcgcgtca
      721 acgtgcccga ctccgagcac tatctggccg acctggaggc ggcctttgcc agcgatatcg
      781 acggtgtcgt cgtccccaag gtggagacga ccgagcaggt cttcccggtc ttgcactgga
      841 tacgccagcg cgacgcccag gcgccgcgcg cgcagccgcg cgcgctgatc ggcggcatcg
      901 agtcggtgcg cggcgtgctc aacgccgtgg cgctgtgtcg cgatatcgat gcactgagcg
      961 cggtgttttt tggcgccgag gacttcgctg ccgacatcgg cgggcgccgc acgccgcgcg
     1021 gcgacgaggt ctacaccgcc cgctccatgg tggtgatggc agcccgggcg gccgggcgca
     1081 ttgccatcga ccaggcggtg atcgacatcc gcaacgacgc gctgtttctg gacgacgccg
     1141 gccgcggccg tgacctgggc tacgagggca agatctgtgt gacgcccggc caggtcaagc
     1201 tggcgcaccg cgcgttttcg cccacacccg aagagcggaa ctacgcgcag cgcctggttg
     1261 agacctacga agccgccatg gcgcgggggc tgggcacgat cgacttcgag ggccgcatga
     1321 tcgacgggcc caccttgaag cgcgcgcagc aggtgctggc cctgccggtg caggaggcgt
     1381 gatgcagctc gaatccctcg atagtctgga tctggcgcgc catgtgcgac cgggcgacgg
     1441 cgtgatcttc cagcagggct gcggcgaggc gctcagcgtg gccgagcggt tcgtgcagca
     1501 gcgcgccagc tacagcggtg cacggctgtt tctgggctcg ggcttttcgc gcagcttcca
     1561 gcccgagcac gccgaccacc tgcgcttttc cggcatcggc ggcatcggca gcctgcgcaa
     1621 gctcgccagc gccggcgcac tcgatcccat tccctgccac atctcctcga tcgagccgct
     1681 gctgcgccag ggcgtgatcg cctcggacgt ggtgatgctg caggtcagcc cccccaacga
     1741 gcgcggcgaa tacagcctgg gcctggtcaa cgactacgtg cgcaccgcca tcggccgggc
     1801 gcgcgtggtg attgccgaga tcaaccgcca gatcccctgg gtgcattgcg acctgccgct
     1861 cacggcggcc gacatcagcg tggcggtgga aaccgaccgc gcgccgctgg agctgccgcc
     1921 cgcgcccttc ggcgcgctgg agcagcgcat cgcgcagcac atcggcgatc tgatcggcga
     1981 tcgctccacg ctgcagatgg gcattggcag cgtgcccgag gccatcatga gctcgctggc
     2041 cgaccggcgc gagctgggca tccattcggg catgatcggc gactcggccg tgacgctgat
     2101 ggagtgcggc gccgtcacca acgcgcacaa gggcatggat gcgggcgtca ccgtcagcgg
     2161 cgtgctgttc ggcacgcaaa agctctaccg ctatgccgag cgcaacccgc agatccggct
     2221 gcacccgacc agctacacgc accacatcgg cgtgctggcg cggctcaaga acttcgtctc
     2281 gatcaattcg gcgctggagg tggacctgac gggccagatc aacgccgagg ccattgggcg
     2341 cgagcacatt ggcgccgtcg gtgggcaggt cgactacatc cgcgccgccg cgctgtccga
     2401 aggcggcgtc tccatcgtgg ccttgccggc cacgggaaaa ggcggcgact cgaagatcgt
     2461 cagccgcctg tccggccccg tgaccaccgc gcgcagcgac gtcgatgtgg tggccaccga
     2521 gcacggcgtg gcgca
//
LOCUS       DAOOOG010000281         2509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_51318_length_2509_cov_25.979218, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000281 DAOOOG010000000
VERSION     DAOOOG010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51318_length_2509_cov_25.979218"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /gene="mpl"
                     /locus_tag="PK242_13345"
     CDS             <1..1352
                     /gene="mpl"
                     /locus_tag="PK242_13345"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HOZ95050.1"
                     /translation="AGHRVTGCDAGVYPPMSDQLRALGIELIEGYGADQLALRPDMFV
                     IGNVVSRARLPDGSPKYPLMEAILDAGAPYTSGPQWLAEHVLAGRHVLAVAGTHGKTT
                     TTSMLAWILESAGLSPGFLVGGVPLNFGISARLQGQAPGQPRPLFVIEADEYDTAFFD
                     KRSKFVHYRPRTAVLNNLEFDHADIFDDLAAIERQFHHLVRTVPPSGRVIVNDLEESL
                     TRVLAQGCWSERRGFGGAISDFMALGEASDFEVQQRGRPVGRVRWSLSGQHNQLNALA
                     AIAAAEHVGVDPATACAALAQFQNVRRRMEVRGRVAVAGGEVTVYDDFAHHPTAIRTT
                     VDGLRRQLDAAGQAGQRILAVFEPRSNTMKLGTMKSQLPWALEHADLSVCHTGGLDWD
                     AAAALSPLGARAQLAGSIDEVVQQVSQAARAGDHVLCMSNGGFGGVHGRLLESLQKR"
     gene            complement(1401..1712)
                     /locus_tag="PK242_13350"
     CDS             complement(1401..1712)
                     /locus_tag="PK242_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95051.1"
                     /translation="MQSEVRVIQDERTEWRFDLDHAAPMDNAQARAWLDAQFIQLGGE
                     PLRPTGKLLLADKVLVVAREAGARRLDDPQWGQDFARAASAALAKPVVRIDLAAMTVS
                     Y"
     gene            complement(1821..>2509)
                     /locus_tag="PK242_13355"
     CDS             complement(1821..>2509)
                     /locus_tag="PK242_13355"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOZ95052.1"
                     /translation="QLLIDINRLGLPAGSEFLDVISPQYIGDLISWGAIGARTTESQV
                     HRELASGLSAPIGFKNGTDGNIRIATDAIQAAARGHHFLSVHKNGQVAIVHTDGNKDC
                     HVILRGGKAPNYDAASVDAACKELAASKLHASLMVDCSHANSSKQHEKQLDVARDVAA
                     QVSAGSRGVFGVMVESHIHGGAQKFTPGKDQVASLEYGKSITDACIGWDGTQTILQVL
                     SDAVRARRGR"
BASE COUNT          342 a          862 c          899 g          406 t
ORIGIN      
        1 aggccggcca ccgcgtcacc ggctgcgacg ccggcgtcta cccgccgatg agcgaccagc
       61 tgcgcgcgct gggcatcgag ctgatcgagg gctacggcgc cgaccagctg gcgctgcggc
      121 ccgacatgtt cgtgatcggc aacgtggtga gccgggcgcg gctgcccgac ggttcgccca
      181 agtacccgct gatggaggcg atcctcgacg ccggcgcgcc ctacaccagc ggcccgcagt
      241 ggctggccga gcatgtgctg gccggccgcc acgtgctggc ggtggccggc acgcacggca
      301 agaccaccac cacgtcgatg ctggcgtgga ttctggaaag cgccgggctg tcacccggct
      361 ttctggtggg cggggtgccg ctgaacttcg gcatttcggc gcgtttgcag ggtcaggccc
      421 ccggccagcc gcgaccgctg ttcgtcatcg aggcggacga atacgacacc gccttcttcg
      481 acaagcgcag caagttcgtg cactaccgcc cgcgcacggc ggtgctgaac aacctggagt
      541 tcgaccacgc cgacatcttt gacgacctgg cggcgatcga gcgccagttc caccacctgg
      601 tgcgcaccgt gccgccttcg ggccgcgtca tcgtcaacga cctggaagaa agcctgacgc
      661 gggtgctggc ccagggctgc tggagcgagc ggcgcggctt tggtggcgcg atcagcgatt
      721 tcatggcgct gggcgaggcc tcggacttcg aggtgcagca gcgcgggcgc cccgtcggcc
      781 gcgtgcgctg gtcgctgtcg ggtcagcaca accagctcaa tgcgctggcc gcgattgccg
      841 cggccgagca cgtgggggtg gatccggcca ccgcctgcgc ggcgctggcg cagttccaga
      901 acgtgcgccg gcgcatggag gtgcgcggcc gcgtggcggt ggcgggcggc gaggtgacgg
      961 tgtacgacga ctttgcccac catccgaccg ccattcgcac cacggtcgac ggcctgcgcc
     1021 gccagctcga cgccgccggc caggccgggc agcgcatcct ggcggtgttc gagccgcgca
     1081 gcaacaccat gaagctgggc accatgaagt cgcaactgcc ctgggcgctg gaacacgccg
     1141 acctgagcgt ctgccacacc ggcgggctgg actgggatgc cgcagcggcg ctgtcaccgc
     1201 tgggcgcgcg cgcccagctg gcaggcagca tcgatgaggt cgtgcagcag gtcagccagg
     1261 ccgcgcgggc cggtgaccac gtgctgtgca tgagcaacgg cggcttcggc ggcgtgcacg
     1321 gccggctgct tgaatcgctt caaaaaagat agctgctggc gcgcgctgga caggcgttat
     1381 cgcctgattt gacttgcgat tcagtacgac accgtcatcg ccgccagatc gatgcgcacc
     1441 accggcttgg ccagcgcagc gctggcggca cgggcgaagt cctggcccca ctgcgggtca
     1501 tccagccgac gcgcgccggc ttcgcgcgcc accaccagca ccttgtcggc cagcagcagc
     1561 ttgcccgtgg ggcgcagcgg ctcgccgccc agctggatga actgcgcgtc cagccaggcg
     1621 cgggcctggg cgttgtccat gggggcggcg tggtcgaggt cgaatcgcca ttcggtgcgt
     1681 tcgtcctgga tgacgcgtac ttcactctgc atgatgggct ggcctcctga gttctgacgg
     1741 gtgcgcgggt ggcggcgcca tgccgcgtgc cgcgcgccca ccgactgtag tcgatcggcg
     1801 gcggccgacc ttgaccggcc tcagcggccg cgtcgcgccc gcacggcgtc ggacagcacc
     1861 tgcaggatgg tctgcgtgcc gtcccagccg atgcaggcgt cggtgatgct cttgccgtac
     1921 tccaggctgg ccacctggtc cttgccgggc gtgaacttct gcgccccgcc gtggatgtgg
     1981 ctttccacca tcacaccgaa cacgccgcgc gagccggcgc tgacctgcgc cgccacgtcg
     2041 cgcgccacgt cgagctgctt ttcgtgctgc ttgctgctgt tggcgtggct gcaatcgacc
     2101 atcagcgagg cgtgcagctt gctggccgcc agctccttgc aggcggcgtc gacgctggcg
     2161 gcgtcgtagt tgggcgcctt gccgccgcgc aggatgacgt ggcagtcctt gttgccgtcg
     2221 gtgtgcacga tggccacctg gccgttcttg tgcaccgaca gaaagtggtg cccgcgcgcc
     2281 gccgcctgga tggcgtcggt ggcgatgcgg atgttgccgt cggtgccgtt cttgaagccg
     2341 atcggtgccg acaggccgct ggccagctcg cggtgcacct ggctttcggt ggtgcgcgcg
     2401 ccgatcgcgc cccagctgat caggtcgccg atgtactggg gcgagatgac atcgaggaac
     2461 tcgctgcccg ccggcaggcc caggcggttg atgtcgatca gcagctggc
//
LOCUS       DAOOOG010000282         2509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_51319_length_2509_cov_24.220049, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000282 DAOOOG010000000
VERSION     DAOOOG010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51319_length_2509_cov_24.220049"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PK242_13360"
     CDS             <1..641
                     /locus_tag="PK242_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOZ95053.1"
                     /translation="LAGPDNRFGDGDIRGVAAPEAANNASAAGSFVPMLTLGVPGSGT
                     TAVMMGALALYNITPGPALFTQNPTLVWGLIASFFVANVLLLIINIPLVGVFARMLQT
                     PSWLLVPAILAVSAVGVYSVHATTFDLVLMSVFGVVGYLLRKQGVPMAPLILGFVLGD
                     MMEQNLRRALSITDGDVGILVESPISIGLWVLSGLMLIVPPLLRLRGRAKAR"
     gene            complement(720..>2509)
                     /locus_tag="PK242_13365"
     CDS             complement(720..>2509)
                     /locus_tag="PK242_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HOZ95054.1"
                     /translation="AIGEARALPDSVQPRDRAGRVDLTDVPLVTIDGEDARDFDDAVY
                     CEPAVVRGRGKRPNGWRLLVAIADVSHYVRTGAPLDVDAYDRATSVYFPRRVIPMLPE
                     KLSNGLCSLNPEVERLSMVCDMLVTAQGEIHAYQFYPAVIRSHARLTYTEVAAILANT
                     RGPEAARRQERVGDLIHLHDVYRALLKARQQRGAVDFDTVETQIVCDEAGRIEKIVPR
                     PRNDAHRLIEECMLAANVCSADFIGLGKHPGLYRVHEGPTPEKVEILRAYLKALGLGL
                     SVSDEPRPEEFQRIAAATAERADAEQIHMMLLRSMSQAIYTPINSGHFGLAYAAYTHF
                     TSPIRRYPDLLVHRVIKAILAERKYQLPTLPTPGEAHEKLSKRLKKDSLSRAQDRRDR
                     PDRLSPEVQAWEAAGLHCSANERRADEASRDVEAWLKCKYMKEHLGEEYAGTVSAATS
                     FGLFVTLSDLHVEGLVHITELGGEYFRFDEIRQELRGERTGIRYAIGTRVRVQVSRVD
                     LDARKIDFRLVREGEESLARVPRDKGAVAGAERGRPAKASSKRAARQQREAERGNARP
                     SPMQALKSAVRKAAAKKGAASGKPRKSRR"
BASE COUNT          319 a          854 c          883 g          453 t
ORIGIN      
        1 gcctggccgg gccggacaac cgcttcggcg acggcgacat ccgcggcgtg gcggccccgg
       61 aggcggccaa caatgcctcg gcggcgggct cgttcgtgcc catgctgacg ctgggcgtgc
      121 ccggctcggg cactacggcg gtgatgatgg gggcgctcgc gctctacaac atcacgcccg
      181 gcccggcgct gttcacgcag aacccgaccc tggtgtgggg cctgatcgcc tcgttcttcg
      241 tcgccaacgt gctgctgctg atcatcaaca tcccgctggt cggcgtgttc gcgcgcatgc
      301 tgcagacgcc cagctggctg ctggtgcccg ccatcctggc ggtcagcgcg gtgggggtgt
      361 actcggtgca cgccaccacc ttcgatctgg tgctgatgtc ggtgttcggc gtggtcggct
      421 acctgctgcg caagcagggc gtgccgatgg caccgctgat cctgggtttc gtgctgggcg
      481 acatgatgga gcagaacctg cgccgcgcac tgtcgatcac cgacggcgac gtcggcatcc
      541 tggtggaaag cccgatttcc atcggtctgt gggtgctcag cggtctgatg ctgatcgtgc
      601 cgccgctgtt gcgcctgcgg gggcgcgcca aggcccgctg aggccttgaa cagcagcaaa
      661 actgcttccg tcgcccgccc agtatgcgca agccgctctc attttgagag cggttttttt
      721 caacgccggc tcttgcgggg tttgccgctg gcggcgccct tcttggccgc cgccttgcgc
      781 acggccgact tcagcgcctg catgggtgag ggccgcgcgt tgccgcgttc ggcctcgcgc
      841 tgctggcgcg cggcgcgctt gctcgaggcc ttggctggcc ggccgcgctc ggcgcctgcc
      901 accgcgccct tgtcgcgggg gacgcgcgcc agactttcct cgccttcacg caccagccgg
      961 aagtcgatct tgcgcgcgtc cagatcgacc cggctgacct gcacccgcac ccgggtgccg
     1021 atggcgtaac ggatgccggt gcgctcgccg cgcagttcct ggcggatctc gtcgaagcgg
     1081 aagtattccc cgcccagctc ggtgatgtgc accaggcctt cgacgtgcag gtcgctgagg
     1141 gtgacgaaca ggccgaagct ggtggctgcg ctgacggtgc cggcgtattc ctcgcccagg
     1201 tgctccttca tgtacttgca cttgagccag gcctcgacgt cgcggctggc ttcgtcggca
     1261 cggcgctcgt tggcgctgca atgcaggccc gccgcctccc acgcctgcac ctcgggcgac
     1321 aggcgatccg gcctgtcacg ccggtcctgc gcgcgcgaca ggctgtcctt cttcaggcgc
     1381 ttgctcagct tttcatgggc ttcgccgggc gtgggcaggg tcggcaactg gtacttgcgc
     1441 tcggccagga tggccttgat gacgcggtgc accagcagat cggggtagcg ccggatcggg
     1501 ctggtgaaat gcgtgtaggc ggcatatgcc agtccgaaat gcccgctgtt gatgggcgtg
     1561 tagatggcct ggctcatgga acgcagcagc atcatgtgga tctgctcggc gtcggcgcgc
     1621 tcggcggtgg cggcggcgat gcgctggaat tcctcgggcc gcggctcgtc gctgaccgac
     1681 aggcccaggc ccagcgcctt caggtaggcg cgcaggatct cgaccttctc cggcgtcggc
     1741 ccttcgtgca cgcggtacag accggggtgc ttgcccaggc cgatgaagtc ggcgctgcac
     1801 acgttggcgg ccagcatgca ttcctcgatc aggcggtgcg cgtcgttgcg cgggcgcggc
     1861 acgatctttt cgatgcggcc ggcctcgtcg cagacgatct gcgtctccac ggtatcgaag
     1921 tccaccgcgc cgcgctgctg gcgcgccttc agcaaggcgc ggtacacgtc gtgcaggtga
     1981 atcaggtcgc ccacgcgctc ctgccgccgc gccgcctcgg gcccgcgggt gttggccagg
     2041 atggccgcca cctcggtgta ggtcaggcgt gcatggctgc gaatcaccgc ggggtagaac
     2101 tggtaggcgt ggatctcgcc ctgcgccgtc accagcatgt cgcacaccat gctcaggcgc
     2161 tccacctcgg ggttgagcga gcacagaccg ttgctgagct tttccggcag catgggaatg
     2221 acgcgacgcg gaaagtacac gctggtggcg cggtcgtagg cgtccacgtc gagcggcgcg
     2281 ccggtgcgca cgtagtggct gacgtcggcg atcgccacca gcaggcgcca gccgttgggc
     2341 cgcttgccgc gcccgcgcac caccgccggc tcgcagtaga cggcgtcgtc gaaatcacgc
     2401 gcgtcttcgc cgtcgatggt gaccagcggc acatccgtca ggtccacgcg gccagcgcgg
     2461 tcgcgcggct gcacgctgtc gggcagggcg cgcgcctcgc cgatggcgg
//
LOCUS       DAOOOG010000283         2508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_51357_length_2508_cov_19.531594, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000283 DAOOOG010000000
VERSION     DAOOOG010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51357_length_2508_cov_19.531594"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PK242_13370"
     CDS             <1..1027
                     /locus_tag="PK242_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ95055.1"
                     /translation="SLVGALPDATAVRNTDVVLPVTPMFHANSWGMPYIAVMLGAKLV
                     FPGPHLHPEDLLDLMQQEPPTLTLGVPTIWLAMIQLYDSSLQPGSPHHGRWQLPTGLR
                     SMVGGAAVPESLIRAFGRHGVWIMQGWGMTETSPVGTLAYPRAELQGASDDERLRRAA
                     MAGVPMPLVDLRIRGDHGQDQPWDGTSVGELQVRGPFITGGYHEAGAPPDKFTADGWL
                     RTGDVASVDALGYMRISDRTKDLIKSGGEWISSVDLENALMAHPAVAEAAVIAIPDEK
                     WSERPLACVVFKPGQQATHEDMNALLLTKGFAKWQLPERYEVIDAVPRTSTGKFYKLQ
                     LRERFPA"
     gene            complement(1355..2389)
                     /locus_tag="PK242_13375"
     CDS             complement(1355..2389)
                     /locus_tag="PK242_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HOZ95056.1"
                     /translation="MAQDPDASVVGGDPAPTPPRPPKLLERMRQHLRTRHYSIRTEEA
                     YIDWARRFILFHDKRHPQDMGAAEVGVFLTHLAVDRQVSASTQNQAKAALLYLYKQVL
                     GVDLPWLDEVVQAKRPRRLPVVLTPSEVRTLLQHMDGTTGLIAELLYGTGMRLLEGLR
                     LRVKDIEFARREIVVREGKGGKDRVTVLPENLIAPLQAQLHKARALHEKDLAAGLGRV
                     YLPHALAVKYPGADRTWAWQWVFPSPVRAIDPRPDARTGQPLERRHHVYPESVQRAVR
                     EAARVAQIAKPVSPHVLRHSFATHLLQAGYDIRTVQELLGHADVNTTMIYTHVLNKGG
                     RGILSPLDAL"
BASE COUNT          415 a          894 c          804 g          395 t
ORIGIN      
        1 ttcgctggtc ggcgccctgc ccgacgccac cgccgtgcgc aacaccgacg tggtgctgcc
       61 ggtcaccccc atgttccacg ccaacagctg gggcatgccc tacatcgcgg tgatgctggg
      121 cgccaagctg gtgtttcccg gcccgcacct gcaccccgaa gacctgctgg acctgatgca
      181 gcaggagccg cccacgctga ccctgggggt gccgaccatc tggctggcga tgatccagct
      241 ctacgattcg tcgctgcagc ctggctcgcc gcaccacggg cgctggcagc tgccgaccgg
      301 cctgcgctcc atggtcggcg gcgcggcggt gcccgaaagc ctgatccgcg ccttcggccg
      361 ccacggcgtg tggatcatgc agggctgggg catgaccgaa accagcccgg tcggcaccct
      421 ggcctacccg cgcgccgagc tgcagggcgc cagcgacgat gaacgcttgc gccgcgccgc
      481 catggccggt gtgccgatgc cgctggtcga cctgcgcatc cgtggcgacc acggccagga
      541 ccagccctgg gacggcacca gcgtgggcga gttgcaggtg cgcggaccct tcatcaccgg
      601 cggctaccac gaagccggcg cgccgcccga caaattcacc gccgacggct ggctgcgcac
      661 cggcgacgtc gccagcgtgg acgcgctggg ctacatgcgc atcagcgacc gcaccaagga
      721 cctgatcaag tccggcggcg aatggatcag ctcggtcgat ctggaaaacg ccctcatggc
      781 ccacccggcg gtggccgaag cggcggtgat cgccatcccg gacgaaaaat ggagtgagcg
      841 cccgctcgcc tgcgtggtat tcaaacccgg ccagcaagcc acgcacgaag acatgaacgc
      901 cctgctgctg accaaaggtt tcgccaagtg gcaactgccc gagcgctacg aggtcatcga
      961 cgccgtgccg cgcacctcga cgggcaagtt ctacaagctg cagctgcgcg agcgcttccc
     1021 cgcatagtcc actgaaccct gccgtgttta gtgctgtcag gcacttatag aagccagaac
     1081 aaggcttgcg gcaagacgcg gctcgcagcc catgctggcg gccttggcaa gaggcgcaac
     1141 gccgccgcaa gcctttttct ggcttgccgc agggcgagcc gccagggggc catctgcttt
     1201 gtggcgtggc cttgacatgg ctccaccatg cctgcggcca cgcgccgcgc agctggcccc
     1261 cgggcgactc gcaatgggta cctggcagcg cgaaacaccg cactggttgt agtcgcccgg
     1321 cagggccggc ccggcacagc cgccggcccg cccctcacag cgcgtccagc gggctcaaaa
     1381 tgccccgccc gcccttgttc agcacatggg tgtagatcat ggtggtgttc acatcggcat
     1441 ggcccagcag ctcctgcacg gtgcgaatgt cataacccgc ctgcagcaga tgcgtggcaa
     1501 acgaatggcg cagcacatgc ggcgacaccg gcttggcaat ctgcgccacg cgcgccgcct
     1561 cgcgcaccgc gcgttgcacc gactcggggt acacatggtg gcgccgctcc agcggctggc
     1621 cggtgcgcgc atccgggcgc gggtcgatgg cccgcaccgg tgacggaaac acccactgcc
     1681 aggcccaggt gcggtcggcg ccggggtatt tgacggccag cgcgtgcggc agatagaccc
     1741 gccccagccc ggccgccaga tccttctcgt gcaacgcccg cgccttgtgc aactgcgcct
     1801 gcagcggcgc gatcaggttt tcgggcagca ccgtcacccg gtccttgccg cccttgccct
     1861 cgcgcaccac gatttcgcgc cgcgcaaatt cgatgtcctt cacacgcagg cgcagcccct
     1921 ccagcagccg catgcccgtg ccatacagca gctcggcaat caggcccgtg gtgccgtcca
     1981 tgtgctgcaa cagcgtgcgc acttcgctgg gtgtcagcac caccggcaga cggcgcgggc
     2041 gtttggcctg caccacttcg tcaagccagg gcaaatccac ccccagcacc tgcttataca
     2101 gatacagcag cgccgccttg gcctggttct gcgtggaggc ggacacctgc cgatccaccg
     2161 ccaggtgggt caagaacacc ccgacctcgg ccgcccccat gtcctgcggg tggcgtttat
     2221 cgtgaaaaag tatgaagcgc cgcgcccaat cgatatacgc ctcctcggtg cggatgctgt
     2281 aatggcgcgt gcgcaggtgc tggcgcatgc gctccagcag cttgggcggg cgcggcggcg
     2341 tgggggcggg gtctccgccc acgaccgacg catcggggtc ctgggccatc cgggaaattt
     2401 taggtttgac taaacgcaac cgatctaggg tttacgtggg gatgttgcaa gatgtattgt
     2461 gttgggaggc atcctgcgct accatcccca tcgtctaatc ctagttag
//
LOCUS       DAOOOG010000284         2498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_51640_length_2498_cov_20.541957, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000284 DAOOOG010000000
VERSION     DAOOOG010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51640_length_2498_cov_20.541957"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PK242_13380"
     CDS             complement(<1..408)
                     /locus_tag="PK242_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOZ95057.1"
                     /translation="MPDYAFVFAGFAVGLIVGLTGVGGGSLMTPILIFFFGVKPYLAV
                     GTDLLFAAFTKLGGTVSLARQRLVPWRVVALLCAGSIPAALAALWALRHLGPDSAAAQ
                     RLMTSTLGVALLLTAAATFYKVLVFSPERAAAEA"
     gene            571..1800
                     /gene="pncB"
                     /locus_tag="PK242_13385"
     CDS             571..1800
                     /gene="pncB"
                     /locus_tag="PK242_13385"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOZ95058.1"
                     /translation="MPGIIPSLLDTDLYKFTMMQAVLHQFPGAQVQYKYKCRTPGVQL
                     TPYLAEIEAEIRQLCELRFTDAELDYMRGLRFIKSDFVDFLGLFRLNRKYITLTPLDN
                     GELDIRVRGPWLHTILFEIPVLAIVSEVYYRNTQPGLGRAEGRRRLDTKIAQLQAPGL
                     QELKIADYGTRRRFSLDWQREVLHTLKARLGSQADGQLTGTSNVMLARELGLRPMGTM
                     AHEYLQACQALGPRLRDTQVFGFEKWAQEYRGDLGIALSDVYGMNAFLRDFDMYFCKL
                     FDGARHDSGDPFQWGERMIEHYRANRCDPRTKVLVFSDALTIPRCIELHQRFQGRIQT
                     AFGVGTNLTNDLGPEALQIVLKMIECNGQPVAKISDAPGKTMVEDEGYLKYLRQVFDL
                     PAPPPTGPLRRTLRGAL"
     gene            1967..>2498
                     /locus_tag="PK242_13390"
     CDS             1967..>2498
                     /locus_tag="PK242_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOZ95059.1"
                     /translation="MLGAPAQAAALDGSQLSPMWAVPFVGILLSIAIWPLAGPHVWHH
                     HFGKIAAGWALLFLLPFAYVFGPGAAAAGLVHALLAEYLPFILLLTALFTVSGGIYIR
                     GNLKGSPLLNTAMLAIGGMLASFMGTTGASMLLIRPLIRANDNRARNAHVVIFFIFIV
                     SNIGGSLTPLGDPPLFL"
BASE COUNT          398 a          875 c          816 g          409 t
ORIGIN      
        1 ggcctcggcg gccgcgcgct cgggcgagaa caccagcacc ttgtagaagg tggccgcggc
       61 cgtgagcagc agggccacgc ccagggtgct ggtcatgagg cgctgggcgg cggcgctgtc
      121 cggccccagg tggcgcagcg cccacagggc ggccagggcg gccgggatgc tgcccgcgca
      181 cagcagcgcc accacgcgcc agggcacgag gcgctggcgc gccaggctca cggtgccgcc
      241 gagcttggtg aaggccgcaa acagcaggtc cgtgcccacg gccagatagg gcttgacgcc
      301 gaagaagaag atcaggatgg gcgtcatcag cgagccgccg cccacgcccg tcagccccac
      361 gatgaggccg acggcgaaac ccgcaaagac aaaggcgtaa tccggcatgg gcgcgactct
      421 aggcgcgctt tatgcggcaa caaacgattt tttcgttgtt cttatattcg ccaagactat
      481 ttggcgggcg gccggcggcg gcaatggggc tacactggcg cgcaccggcc ctctgcgcgg
      541 gcggcccgac gagagacacg agacacgccc atgcccggca tcatccccag cctgctcgac
      601 accgatctgt acaagttcac catgatgcag gcggtgctgc atcagtttcc gggcgcgcag
      661 gtgcagtaca agtacaaatg ccgcacgccg ggcgtgcagc tgacgcccta cctggccgag
      721 atcgaggccg agatccgcca gctgtgcgag ctgcgcttca ccgacgccga gctggactac
      781 atgcgcggcc tgcgcttcat caagagcgat ttcgtcgatt ttctgggcct gtttcgcctc
      841 aaccggaagt acatcaccct gaccccgctg gacaacggcg agctcgacat ccgtgtgcgc
      901 ggcccctggt tgcacaccat cctgttcgag atcccggtgc tggccatcgt cagcgaggtc
      961 tactaccgca acacccagcc cgggctgggc cgcgccgaag gccgccgtcg cctggacacc
     1021 aagatcgccc agttgcaggc ccccggcctg caggagctca agatcgccga ctacggcacg
     1081 cgccggcgct tttcgctcga ttggcagcgc gaggtgctgc acaccctgaa ggcgcgtctg
     1141 ggcagccagg ccgacggcca gctcaccggc accagcaacg tcatgctggc gcgcgagctg
     1201 ggcctgcgcc ccatgggcac catggcgcac gaatacctgc aggcctgcca ggcgctgggc
     1261 ccgcggctgc gcgacaccca ggtgttcggc ttcgagaaat gggcgcagga ataccgcggc
     1321 gatctgggca tcgcgctgtc cgacgtgtac ggcatgaacg ccttcctgcg cgacttcgac
     1381 atgtacttct gcaagctgtt cgacggcgcg cgccacgact cgggtgaccc gttccagtgg
     1441 ggcgagcgca tgatcgagca ctaccgcgcc aaccggtgcg acccgcgcac caaggtgctg
     1501 gtgttcagtg acgcgctcac catcccgcgc tgcatcgagc tgcaccagcg ctttcagggt
     1561 cgcatccaga ccgcgttcgg cgtcggcacc aacctgacca acgacctcgg ccccgaggcg
     1621 ctgcagatcg tgctgaagat gatcgagtgc aacggccagc cggtggccaa gatctccgac
     1681 gccccgggca agaccatggt cgaggacgag ggctacctga agtatctgcg ccaggtgttc
     1741 gacctgccgg cgccgccgcc gaccgggccg ctgcgacgca ccctgcgggg cgcgctgtga
     1801 gtcccgggtt gctatgaatt agatagctgt tggcgcttgc cagataagcg ctggaagtgt
     1861 ttttgatgtt tgtcttgacg acgctccaac catgaccgca tgctccgccc cgtccctgcg
     1921 tcgcgtgctc cagcccgcgc ggctggcgct gttggcctgg ctgctgatgc tgggcgcgcc
     1981 ggcgcaggcc gccgcgctcg acggcagcca gctgtcgccc atgtgggccg tgcccttcgt
     2041 cggcatcctg ctgtcgatcg ccatctggcc gctggccggg ccgcatgtct ggcaccacca
     2101 cttcggcaag atcgccgccg gctgggcgct gctgtttctg ctgccgtttg cctacgtgtt
     2161 cggccccggt gcggcggcgg ccggcctggt gcatgcgctg ctggccgaat acctgccctt
     2221 catcctgctg ctgacggcgc tgttcacggt ctcgggcggc atctacatcc gcggcaacct
     2281 caagggctcg ccgctgctca acaccgccat gctggccatc ggcggcatgc tggccagctt
     2341 catgggcacc accggggcgt cgatgctgct gatccgcccg ctgatccgcg ccaacgacaa
     2401 ccgggcgcgc aacgcgcacg tggtgatttt cttcatcttc atcgtcagca acatcggcgg
     2461 ctcgctcacg ccgctgggcg atccgccgct gttcctgg
//
LOCUS       DAOOOG010000285         2477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_52239_length_2477_cov_27.986375, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000285 DAOOOG010000000
VERSION     DAOOOG010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52239_length_2477_cov_27.986375"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..532
                     /locus_tag="PK242_13395"
     CDS             188..532
                     /locus_tag="PK242_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95060.1"
                     /translation="MKTLQAPTRSAAVLAQLLERLDASKQPVDAHQYRTVAARLADML
                     ADADTDWQPLLQQSPAAAALYENLHYADAGLCRSPLDAAMRAEMSARDAIETARRRPA
                     SPDTAADAASAG"
     gene            548..994
                     /gene="trxC"
                     /locus_tag="PK242_13400"
     CDS             548..994
                     /gene="trxC"
                     /locus_tag="PK242_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HOZ95061.1"
                     /translation="MSEPLHIVCPHCHTTNRVKADQLGSAPDCGQCKQPLFTGHPVAL
                     DDEAAFDKHVTRSQVPVLVDFWAPWCGPCRMMAPAYEKAAGDLEPRVRVAKVNTEAAQ
                     ALGGRYNIRSIPTLVLFVGGKEVARQPGAMTNPAQIVQWVNAHLPR"
     gene            complement(1022..1471)
                     /gene="nrdR"
                     /locus_tag="PK242_13405"
     CDS             complement(1022..1471)
                     /gene="nrdR"
                     /locus_tag="PK242_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOZ95062.1"
                     /translation="MRCPFCAHTETQVVDSRVSDEGDFIRRRRQCAHCGKRFTTYERP
                     DVSYPSIIKKDGRRVPYARAKLQGSLQLALRKRPVSTEQQDAAIERIEEKLLATGARE
                     VSSSRIGELVMRELKKLDKVAYVRFASVYRSFEDIDEFRALVDEVRR"
     gene            complement(1605..>2477)
                     /gene="glyA"
                     /locus_tag="PK242_13410"
     CDS             complement(1605..>2477)
                     /gene="glyA"
                     /locus_tag="PK242_13410"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOZ95063.1"
                     /translation="HLTHGMPLNMSGKWFNVVSYGLNAQEAIDYDAMEKKAHETKPKL
                     IIAGASAYSLEIDFARFAKVAKDVGAIFMVDMAHYAGLIAGGQYPNPVPHADVVTSTT
                     HKSLRGPRSGFILMKAEHEKAINSAVFPGLQGGPLMHIIAAKAIAFNEALQPEFKQYA
                     QQVKKNAKVVADTLTERGLRIVSGGTQSHVMLVDLRAKGITGKEAEAVLGSAHMTINK
                     NAIPNDPEKPMVTSGVRIGTPAMTTRGFKEDEARATAHLVADVLDQPRNEAHIAAVRA
                     KVAELTKRFPVYGN"
BASE COUNT          391 a          825 c          810 g          451 t
ORIGIN      
        1 tgccgagaca aagaaagtga ctgcgccgcc gggcgcacat cccggccgac agcgcctccc
       61 tcaccccaac ccccatccag cgcgctaaac atcagcgcag aaagctatca aaacaatagt
      121 gaccgcgcgc aatcaagaca ccaccccaca tcttgcattc ccccctccag cccccacctg
      181 ccacctcatg aagacattgc aagcccccac ccgaagcgct gccgttctgg cccagctgct
      241 ggagcggctg gatgccagca agcagcctgt tgatgcgcac cagtaccgca ccgtcgccgc
      301 gcgcctggcc gacatgctgg ccgatgccga caccgactgg cagcccctgc tgcagcaaag
      361 tcccgccgcg gcggcgctgt acgagaatct gcactacgcc gatgccggcc tgtgccgttc
      421 gccactggat gccgccatgc gcgccgaaat gagcgcgcgc gatgccatcg agacggcgcg
      481 tcgccggcct gcgtcgcccg acacggcggc cgacgccgcc tccgctggct gacccctgga
      541 gccgatcatg agcgaacccc tgcacatcgt ctgtccgcat tgccacacca ccaaccgggt
      601 caaggccgac caactgggca gcgcgcccga ttgcgggcaa tgcaagcagc cgctgttcac
      661 gggccacccg gtcgcgctgg acgatgaggc cgcgttcgac aagcacgtca cgcgcagcca
      721 ggtgccggtg ctggtcgact tctgggcgcc ctggtgcggc ccctgccgca tgatggcgcc
      781 cgcctacgaa aaggccgcgg gcgacctgga gccgcgcgtg cgcgtggcca aggtcaacac
      841 cgaggcggcg caggcgttgg gtggccgcta caacatccgc agcattccga cgctggtgct
      901 gttcgtcggc ggcaaggaag tggcgcgtca gccgggcgcc atgaccaacc cggcgcagat
      961 cgtgcagtgg gtcaacgcgc acctgccgcg ttgatgcggc gctgaagccg cggggcggcg
     1021 gtcagcgccg cacttcgtcc accagcgcgc gaaactcgtc gatgtcctcg aagctgcggt
     1081 acacgctggc aaagcgcacg taggccacct tgtcgagctt tttcagctcg cgcatcacca
     1141 gctcgccgat gcggctggac gagacttcgc gcgcgccggt ggccagcagt ttttcctcga
     1201 tccgttcgat ggcggcgtcc tgctgttcgg tgctgaccgg gcgcttgcgc agcgccagtt
     1261 gcagcgagcc ctgcagcttg gcgcgcgcat agggcacgcg ccggccgtcc ttcttgatga
     1321 tgctggggta gctgacgtcc gggcgctcgt aggtggtgaa acgcttgccg caatgcgcgc
     1381 actggcgccg gcggcggatg aagtcaccct cgtccgacac gcggctgtcc accacctggg
     1441 tctcggtgtg ggcacagaag gggcagcgca tctgcaggcg taccgatcaa gaactagcgt
     1501 acgcagagaa gcagagattg ggcagaggac gcaagaaatt catttgggtt tcttctgcgt
     1561 cctctgcgaa tcctttgcgt cctttgcgta gggcggttgg ttttttagtt gccgtacacc
     1621 ggaaagcgct tcgtcagctc ggccaccttg gcgcgcacgg cggcgatgtg ggcctcgttg
     1681 cgcggttggt ccagcacgtc ggccaccagg tgcgcggtgg cgcgcgcttc gtcttccttg
     1741 aagccgcgcg tggtcatggc cggggtgccg atgcgcacgc cgctggtgac catgggcttt
     1801 tccgggtcgt tggggatggc gttcttgttg atcgtcatgt gggcgctgcc cagcacggcc
     1861 tcggcttcct tgccggtgat gcccttggcg cgcaggtcaa ccagcatgac gtggctttgc
     1921 gtgccgccgc tgacgatgcg caggccgcgc tcggtcagtg tgtcggccac caccttggcg
     1981 ttcttcttga cctgctgcgc atattgcttg aattcgggct gcagcgcttc gttgaaggca
     2041 atcgccttgg cggcgatgat gtgcatcagc gggccaccct gcaggccggg gaacacggcg
     2101 ctgttgatgg ctttttcgtg ctcggccttc atcaggatga agcccgagcg cgggccgcgc
     2161 aggctcttgt gcgtggtgct ggtcaccacg tcggcgtgcg gcacggggtt ggggtactgg
     2221 ccaccggcga tcaggccggc gtagtgcgcc atgtcgacca tgaaaatggc gccgacgtcc
     2281 ttggccacct tggcaaagcg ggcaaagtcg atttccagcg aataggccga ggcgccggca
     2341 atgatgagct tgggcttggt ctcgtgcgcc tttttttcca tcgcgtcgta gtcgatggct
     2401 tcctgcgcgt tcagcccgta tgacaccacg ttgaaccact tgccgctcat gttgagcggc
     2461 atgccgtgcg tcaggtg
//
LOCUS       DAOOOG010000286         2455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_52949_length_2455_cov_27.148750, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000286 DAOOOG010000000
VERSION     DAOOOG010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52949_length_2455_cov_27.148750"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PK242_13415"
     CDS             <1..1064
                     /locus_tag="PK242_13415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ95064.1"
                     /translation="HATAVRDIWQDMMAAVRERQPDARINGVTVQAMARKPRGRELYI
                     GVVTDEPFGPVISFGAGGTLIELYRDHAMELPPLNQFLARRLMERSRVHETLGSWRRA
                     PAVDMAALEQVLLRVSEMVCELPQLREMDINPIIVDEHGVLAVDARIVIGPAAQASPG
                     RAGRYGHLSILPYPARFEQLWPLRGGGEYKVRPIHPDDAQPLQDMMKRLTPESRYFRF
                     VSNMAELPPHMLSRFTLIDYDREMALCAVVEEEVPTEDGGKTTRERIIGVSRYITNPD
                     QTSCEFSLVVDDGYAGQGLGTRLMLSIMDAAREKGLQEIIGLVLTHNNGMLKLMRSLG
                     FEVKAYPEDPDFRLVTHAL"
     gene            complement(1180..1638)
                     /locus_tag="PK242_13420"
     CDS             complement(1180..1638)
                     /locus_tag="PK242_13420"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOZ95065.1"
                     /translation="MPFSNPDQALIHRFIADGSRPMAFDANPMARALGAELRAADLRA
                     GTVTLAFAPDPLFIQGTGVLQGGAVTAMLDFAMAFATLAALPVGGSCATVNLNTAFLR
                     PAPRGRYLAVGEVERRGRQLAFTHARLLREDDGQTVATATSTLALVQPDS"
     gene            complement(1672..>2455)
                     /locus_tag="PK242_13425"
     CDS             complement(1672..>2455)
                     /locus_tag="PK242_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ95066.1"
                     /translation="AIAMTEPGTGSDVAAIRTQARREGGHYVLSGQKTFITNGHNADV
                     IVVACKTDPDKGAHGVSLLVVERGMAGFTRGRNLRKIGQHAQDTAELFFDQVRVPASH
                     LLGEEGQGFKYLMQKLAQERLLVSVGCQARAEAALDWTVAYVQERKAFRQRVADFQNT
                     RFKLAQLATDITAGRAYCDQLIARHLAGGLDAVGAAAGKLWHSELLGRVTDECLQLFG
                     GYGYMHEYPIARAYTDARIERIYAGTSEIMKEIVARDLLDGR"
BASE COUNT          358 a          857 c          878 g          362 t
ORIGIN      
        1 tgcacgccac cgccgtgcgc gacatctggc aggacatgat ggcggcggtg cgcgagcgcc
       61 agcccgacgc gcgcatcaac ggcgtcaccg tgcaggccat ggcgcgcaag ccgcgcgggc
      121 gcgagctgta tatcggcgtg gtgaccgacg agccgttcgg cccggtgatc tccttcggcg
      181 ccggcggcac cctgatcgag ctgtaccgcg accacgccat ggagctgccg ccgctcaacc
      241 agttcctggc gcggcggctg atggagcgct cgcgcgtgca cgagacgctg ggcagctggc
      301 ggcgcgcgcc ggcggtcgac atggcggcgc tggagcaggt gctgctgcgc gtgtccgaaa
      361 tggtgtgcga gctgccgcag ctgcgcgaga tggacatcaa ccccatcatc gtcgacgagc
      421 acggcgtgct ggcggtggac gcgcgcatcg tcatcggccc ggcggcgcag gcctcgcccg
      481 ggcgcgccgg gcgctacggc cacctgtcga tcctgccgta cccggcgcgc ttcgagcagc
      541 tgtggccgct gcgtggcggc ggcgaataca aggtgcgccc gatccacccc gacgacgccc
      601 agccgctgca ggacatgatg aagcgcctga cgcccgagag ccgctacttc cgcttcgtct
      661 ccaacatggc cgagctgccg ccgcacatgc tgtcgcgctt cacgctgatc gactacgacc
      721 gcgagatggc gctgtgcgcc gtggtggaag aggaggtgcc gaccgaggac ggcggcaaga
      781 ccacgcgcga gcgcatcatc ggcgtgtcgc gctacatcac caaccccgac cagaccagct
      841 gcgagttctc gctggtggtg gacgacggct acgccggcca gggcctgggc acgcggctga
      901 tgctcagcat catggacgcg gcgcgcgaaa agggcctgca ggaaatcatc ggcctggtgc
      961 tgacgcacaa caacggcatg ctcaagctga tgcgcagcct gggcttcgag gtcaaggcct
     1021 acccggaaga cccggacttc cggctggtga cgcacgcgct ctgagccacc cggctccgtg
     1081 cggcagtgcg cgcaccgggg cgcgatcggc gtcctcgctg atcgctattg aatcgagagc
     1141 tgctggcgct gtttggacgg gcgatggagc gccatccggc taagaatccg gctgaaccag
     1201 cgccagcgtg gacgtggccg tcgccacggt ttgcccgtcg tcctcgcgca gcaggcgtgc
     1261 gtgcgtgaag gccagctgcc ggccgcgccg ctcgacctcc ccgacggcga gatagcgccc
     1321 ccgcggcgcc gggcgcagga aggcggtgtt cagattgacg gtggcgcagg agccgcccac
     1381 cggcagcgcc gccagcgtgg caaaggccat ggcgaagtcc agcatggccg tcaccgcgcc
     1441 gccctgcagc acgccggtgc cctggatgaa cagcgggtcg ggcgcgaagg ccagcgtcac
     1501 cgtgcccgcc cgcaggtcgg ccgcgcgcaa ctcggcgccc agcgcccgtg ccatcgggtt
     1561 ggcgtcgaac gccatcggcc ggctgccatc cgcgatgaag cggtggatca gcgcctggtc
     1621 ggggttgctg aagggcatgt caggccggtg ggcgatacct gggaagacgg cttacctgcc
     1681 gtccagcagg tcccgcgcca cgatctcctt catgatctcc gaggtgccgg cgtagatgcg
     1741 ctcgatgcgc gcatcggtgt acgcgcgggc gatcgggtat tcgtgcatgt agccgtagcc
     1801 gccgaacagc tgcaggcatt cgtcggtcac gcggcccagc agctcgctgt gccacagctt
     1861 gccggcggcg gcgcccaccg cgtccagccc gccggccagg tgccgcgcga tcagctggtc
     1921 gcagtaggcg cggccggcgg tgatgtcggt ggccagctgg gccagcttga agcgggtgtt
     1981 ctggaagtcg gccacgcgct ggcgaaaggc cttgcgctcc tgcacgtagg ccacggtcca
     2041 gtccagcgcc gcctcggcgc gcgcctggca gcccacgctg accagcagcc gctcctgcgc
     2101 cagcttttgc atcaggtact tgaagccctg gccttcctcg cccagcaggt gactggccgg
     2161 cacgcgcacc tggtcgaaga acagctcggc cgtgtcctgc gcgtgctggc cgatcttgcg
     2221 caggttgcgc ccgcgcgtga agccggccat gccgcgctcg accactagca gcgacacgcc
     2281 gtgcgcgccc ttgtcggggt cggtcttgca ggccaccacg atcacgtcgg cgttgtggcc
     2341 gttggtgatg aaggtcttct ggcccgacag cacgtaatgg ccgccctcgc gccgcgcctg
     2401 ggtgcggatg gccgccacgt cgctgccggt gccgggctcg gtcatggcga tggcg
//
LOCUS       DAOOOG010000287         2450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_53102_length_2450_cov_32.298121, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000287 DAOOOG010000000
VERSION     DAOOOG010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53102_length_2450_cov_32.298121"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="PK242_13430"
     CDS             complement(<1..175)
                     /locus_tag="PK242_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ95067.1"
                     /translation="MSSAFAVLHDVFGYPAFRGQQAEIVQHVIEGGDALVLMPTGGGK
                     SLCYQVPAIVRQRA"
     gene            complement(316..621)
                     /locus_tag="PK242_13435"
     CDS             complement(316..621)
                     /locus_tag="PK242_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOZ95068.1"
                     /translation="MTTWTALVLASAAALAIKLAGYAVPARWLLNPRMPRVAGAMTVA
                     LLAALTVMNTFANGQALALDARLAALAVAALALALRAPFLLVVVLGAAAAALVRALG"
     gene            complement(618..1340)
                     /locus_tag="PK242_13440"
     CDS             complement(618..1340)
                     /locus_tag="PK242_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOZ95069.1"
                     /translation="MTVSHAAPPPSRPPLTPATRMGLSIAVATGLYGVSFGALAVASG
                     LSVAQAMALSLLMFTGGSQFAFIGVLAGGGSAGAAVGAATLLGVRNGVYGLQLKPVLG
                     LRGWRQVLAAQLTIDESTATALAQTDAAEQRRGFWTAGVGVFVLWNLFTGVGALLGDA
                     LGDPRRFGLDGAAVAAFLGLLWPRLRQREAVALALVCGLATALALPQLPPGLPILLAA
                     LLGAAWGWWGRAPRANDDEARA"
     gene            1460..>2450
                     /locus_tag="PK242_13445"
     CDS             1460..>2450
                     /locus_tag="PK242_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOZ95070.1"
                     /translation="MTAPAYTRAAALPAILRQRLVILDGAMGTMIQQLKLSEAQFRGE
                     RFADFAKDVKGNNELLSLTRPDVIQAIHEKYLAAGADLVETNTFGATTIAQADYGMEA
                     LAREMNVASARLARAACDKYSTPDKPRFVAGALGPTPRTASISPDVNDPGARNISFEE
                     LRAAYREQAEGLIEGGADVLLVETIFDTLNAKAALFAIDEAFEHSGESLPIIISGTVT
                     DASGRVLSGQTVTAFWYSVRHARPLAVGLNCALGAALMRPYIQELARAAPDTFISCYP
                     NAGLPNPMSDTGFDETPEVTSRLLHELAAEGLVNIVGGCCGTTPEHIGAIARAV"
BASE COUNT          408 a          949 c          825 g          268 t
ORIGIN      
        1 cggcgcgctg gcgcacgatg gccggcacct gatagcacag cgacttgccg ccgccggtgg
       61 gcatcagcac cagcgcgtcg ccgccctcga tcacgtgctg gacgatctcg gcctgctgac
      121 cgcgaaacgc cgggtagcca aagacatcgt gcagaacggc gaaggcggag gacacgaaaa
      181 tactatgatt tatatagcta ctggcgcttg ctggtcaagc gccagagcct gatatgactt
      241 ggaacgatgg ggcgcgatcg ggcgacgcgg cggccgccaa agctgccgat tgtgcccccg
      301 ggaaggcgtg cggcgtcagc ccagggcgcg caccagcgcc gccgccgccg cgcccagcac
      361 caccaccagc aggaacggcg cgcgcagcgc cagcgccagc gccgccacgg ccagcgcggc
      421 cagccgcgcg tccagcgcca gcgcctgccc gttggcgaag gtgttcatca ccgtcagcgc
      481 cgccagcagc gccaccgtca tggctccggc cacgcgcggc atgcgcgggt tcagcagcca
      541 gcgcgccggc acggcgtagc ccgccagctt gatcgccagc gcggcggcac tggccagcac
      601 gagggccgtc caggtcgtca tgcgcgcgcc tcgtcgtcgt tggcgcgcgg tgcgcgcccc
      661 caccagcccc aggccgcgcc cagcagcgcc gccagcaaaa tcggcagccc cggcggcaac
      721 tgcggcaacg ccagcgccgt cgccagcccg cagaccagtg ccagcgccac cgcctcgcgc
      781 tggcgcaggc gcggccacag caggcccaga aaggccgcca ccgccgcgcc gtccagcccg
      841 aagcggcgcg gatcgcccag cgcgtcgccc agcagcgcgc ccaccccggt gaacaggttc
      901 cacagcacga acacgcccac gcccgcggtc cagaagccgc gccgctgctc ggccgcatcg
      961 gtctgtgcca gcgcggtggc ggtcgattcg tcgatggtca gctgcgccgc cagcacctgg
     1021 cgccagccgc gcaggcccag cacgggcttg agctgcaggc cgtacacccc gttgcgcacc
     1081 cccagcagcg tggccgcgcc gactgccgcg cccgcactgc cgccacccgc cagcacgccg
     1141 atgaaggcga actgcgagcc gccggtgaac atcagcagcg agagcgccat cgcctgcgcc
     1201 acgctcaggc ccgaggccac cgccagcgcg ccaaacgaga cgccgtacag cccggtggcc
     1261 acggcgatcg acaggcccat gcgggtggcg ggggtgagcg gtggccgcga gggtggcggc
     1321 gcggcgtggg aaacagtcat agggcgcaga gtggcagggc gggcggcggg cacaacccgt
     1381 gccaatgtgc cacagcgggc ggcgccgcgg catgaccctg gcgcggcgcg ccgcggcggc
     1441 gattcctaca atcgcgccca tgaccgcccc cgcctacacc cgcgccgccg cgctgccggc
     1501 gatcctgcgc caacgcctcg tgatcctgga cggggccatg ggcaccatga tccagcagct
     1561 caagttgagc gaggcgcagt ttcgcggcga gcgctttgcc gatttcgcca aggacgtgaa
     1621 gggcaacaac gagctgctga gcctgacgcg gcccgacgtg atccaggcga tccacgagaa
     1681 atacctggcc gccggcgccg atctggtcga aaccaacacc ttcggcgcca ccaccatcgc
     1741 ccaggccgac tacggcatgg aagcgctggc gcgcgagatg aacgtggcct cggcccgact
     1801 ggcgcgcgcc gcctgcgaca aatacagcac acccgacaag ccgcgcttcg tcgccggcgc
     1861 tctgggcccg acgccgcgca ccgccagcat cagccccgac gtcaacgacc ccggcgcgcg
     1921 caacatcagc ttcgaggagc tgcgcgccgc ctaccgggag caggccgaag ggttgatcga
     1981 gggcggcgcc gacgtgctgc tggtggaaac catcttcgac accctgaacg ccaaggcggc
     2041 gctgtttgcc atcgacgagg cgttcgagca cagcggcgag tccctgccga tcatcatcag
     2101 cggcaccgtg accgacgcct cgggccgcgt gctgtcgggc cagaccgtca ccgccttctg
     2161 gtacagcgtg cgccacgccc ggccgctggc cgtgggcctg aactgcgcgc tgggcgcggc
     2221 gctgatgcgg ccctacatcc aggagctggc cagggccgcg cccgacacct tcatcagctg
     2281 ctaccccaac gccggcctgc ccaacccgat gagcgacacc ggcttcgacg aaacgcccga
     2341 ggtcaccagc cgcctgctgc acgaactcgc cgccgagggc ctggtcaaca tcgtcggcgg
     2401 ctgctgcggc accacgcccg agcacatcgg cgccatcgcc cgcgccgtgg
//
LOCUS       DAOOOG010000288         2447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_53198_length_2447_cov_24.846990, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000288 DAOOOG010000000
VERSION     DAOOOG010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53198_length_2447_cov_24.846990"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="PK242_13450"
     CDS             <1..147
                     /locus_tag="PK242_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase"
                     /protein_id="HOZ95071.1"
                     /translation="YLCGPPMMIDACITTLMQGRLFERDIYTEKFISAADAQQVRSPL
                     FKKI"
     gene            158..502
                     /locus_tag="PK242_13455"
     CDS             158..502
                     /locus_tag="PK242_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HOZ95072.1"
                     /translation="MAHHTITIEDTGESYRCADHRSVLEGMEALGKKGIPVGCRQGGC
                     GVCKVQILEGQYSKRVMSRAHVSAEEEASGCVLSCRIKPTSDLRLHVVGAMKKNVCRP
                     VSEAASVSTHPT"
     gene            525..1451
                     /locus_tag="PK242_13460"
     CDS             525..1451
                     /locus_tag="PK242_13460"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HOZ95073.1"
                     /translation="MAMTGVLRPGHAQIRVLDLEESVQWYTNVMGLQPMGRDAQGRAY
                     FKTRNERDHHSIILRQADQAGLDFFGYKVLDKATLDDLEKKLQAFGVKTERIPAGDLL
                     ETGERVRFRIPTGHTLELYAEKKDVGNGMGYTDPEVDVLDGPGIRPIRMDHALIYGGD
                     IDGNLKLFTEVLGFTLVERVKLEDGTTDLATWLTCSHKAHDIAMVRHAEDAKLHHVAF
                     QMPSWESVLRAADIMSVNRVSIDIGPTRHGITQGTTIYFFDPSGNRLETFCGGYDHYP
                     DMHPVTWSWEEVGRGIFYHDRKLNDAFLSVVT"
     gene            1461..1895
                     /locus_tag="PK242_13465"
     CDS             1461..1895
                     /locus_tag="PK242_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOZ95074.1"
                     /translation="MARHSVEQRAITAEAAHAAVAAAVAKAGELGIRINVAVTDASGV
                     LAAFLRMPGAFLHSVDIAIDKAYTAAGFGFATGQWAGILRGDEALRLGMPVRPRNVVF
                     GGGLPMREQGVLIGGIGVSGGSAEQDEICARAGLAALGLEAA"
     gene            1927..>2447
                     /locus_tag="PK242_13470"
     CDS             1927..>2447
                     /locus_tag="PK242_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOZ95075.1"
                     /translation="MKEFLNFINGQYVKSASGSTFENRNPVNGSLIGMIHEAGKPEVD
                     AAVAAAKAALTGDWGRISVTERCKLLDAVALEINRRFDDFLQAEIADTGKPHHLASHV
                     DIPRGAANFQIFIDTIKSASTESFNMTTPDGRTALSYGVRVPRGVIAVICPWNLPLLL
                     MTWKVGPALACGNT"
BASE COUNT          459 a          839 c          792 g          357 t
ORIGIN      
        1 tacctgtgcg gtccgccgat gatgatcgac gcctgcatca ccaccctgat gcagggccgg
       61 ctgttcgagc gcgacatcta taccgagaaa ttcatctcgg ccgccgatgc gcagcaggtg
      121 cgcagtccgc tgttcaagaa gatctgaggc ggcgaccgtg gcgcaccaca ccatcaccat
      181 cgaggacacc ggcgaaagct accgctgcgc cgaccaccgc tccgtgctgg aaggcatgga
      241 agccctgggc aagaagggca tccccgtggg ctgccgtcag ggcggctgcg gcgtgtgcaa
      301 ggtgcagatc ctggaaggtc agtacagcaa gcgcgtgatg agccgggccc atgtcagcgc
      361 cgaagaagag gccagcggct gcgtgctgtc gtgccgcatc aagcccacca gcgacctgcg
      421 cctgcacgtc gtcggcgcca tgaagaagaa tgtctgccgt cccgtcagcg aggcggccag
      481 cgtatccacc caccccacct gaaaccaagc gaaggagacc tactatggca atgactggcg
      541 ttctgcgccc tgggcacgcc cagatccgcg tactcgacct ggaagaatcg gtccagtggt
      601 acaccaacgt gatgggcctg cagcccatgg gccgcgatgc ccagggccgc gcctatttca
      661 agacccgcaa cgagcgcgac caccacagca tcatcctgcg ccaggccgac caggccgggc
      721 tggacttctt tggctacaag gtgctcgaca aagccacgct cgatgacctg gagaaaaagc
      781 tccaggcctt tggcgtcaag accgaacgca tccccgccgg tgacctgctg gagaccggcg
      841 agcgcgtgcg cttcaggatt cccaccggcc acacgctgga gctgtacgcc gagaagaagg
      901 acgtgggcaa cggcatgggc tacaccgacc ccgaggtgga cgtgctcgac ggccccggca
      961 tccgcccgat ccgcatggac catgcgctga tctacggcgg cgacatcgac ggcaacctca
     1021 agctgttcac cgaggtgctg ggcttcaccc tggtcgagcg cgtcaagctg gaagacggca
     1081 ccaccgacct ggccacctgg ctgacctgca gccacaaggc gcacgacatc gccatggtgc
     1141 gccacgccga ggatgccaag ctgcaccacg tggcgttcca gatgccgagc tgggaaagcg
     1201 tgctgcgcgc cgccgacatc atgagcgtca accgcgtctc gatcgacatc ggtcccacgc
     1261 gccacggcat cacccagggc acgacgatct acttcttcga ccccagcggc aaccgcctgg
     1321 aaaccttctg cggcggctac gaccattacc ccgacatgca tccggtcacc tggagctggg
     1381 aagaggtcgg gcgcggcatc ttctaccacg accgcaagct gaacgacgcc ttcctgagcg
     1441 tggtgacctg aggcgcgccg atggcacgac acagcgtcga acaacgcgcc atcaccgccg
     1501 aggcggcgca cgccgccgtg gcggcggcgg tggccaaggc cggcgagctg ggcattcgca
     1561 tcaacgtcgc ggtgaccgac gcctcgggcg tgctggcggc ctttctgcgc atgcccggcg
     1621 cctttctgca ctcggtggac atcgccatcg acaaggccta caccgcggcc ggcttcggtt
     1681 ttgccaccgg gcagtgggcc ggtatcttgc gcggcgacga ggcgctgcgc ctgggcatgc
     1741 cggtgcggcc gcgcaacgtg gtgttcggcg gcgggttgcc gatgcgcgag caaggggtgc
     1801 tgatcggcgg cattggcgtg tccggcggat cggccgaaca ggacgagatc tgcgcccgcg
     1861 cgggcctggc cgcgctgggg ctggaggccg cgtgagcggc cgagcttcca ggaacagaga
     1921 gcacccatga aagagttttt gaatttcatc aacggccagt acgtcaagag cgccagcggc
     1981 agcacgttcg agaaccgcaa cccggtcaac ggcagcctga tcggcatgat ccatgaagcc
     2041 ggcaagcccg aagtggacgc cgccgtggcg gccgccaagg ccgcgctcac gggcgactgg
     2101 ggccgcattt cggtcaccga gcgctgcaag ttgctggatg ccgtggcgct cgagatcaac
     2161 cggcgcttcg acgacttcct gcaggccgag atcgccgaca ccggcaagcc gcaccacctg
     2221 gcctcgcacg tggacatccc gcgcggcgcg gccaactttc agatcttcat cgacaccatc
     2281 aagagcgcct ccaccgagtc gttcaacatg accacgcccg acggcaggac cgcgctcagc
     2341 tacggcgtgc gcgtgccgcg cggcgtgatc gcggtgatct gcccctggaa cctgccactg
     2401 ctgctgatga cctggaaggt cggcccggcg ctggcctgcg gcaacac
//
LOCUS       DAOOOG010000289         2422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_53997_length_2422_cov_24.790452, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000289 DAOOOG010000000
VERSION     DAOOOG010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53997_length_2422_cov_24.790452"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK242_13475"
     CDS             <1..354
                     /locus_tag="PK242_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ95076.1"
                     /translation="DIVALEMLVLRVLSAEKSGRNALDIAGLLKIKGSEIQQRYTELM
                     MLAAGPFSLPYIWEAMEAGWQGDQAAAAGLQGFPGGAVANATLAASYFNYRKTTIYGG
                     SNEVQRNIVAQTVLG"
     gene            378..1493
                     /locus_tag="PK242_13480"
     CDS             378..1493
                     /locus_tag="PK242_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ95077.1"
                     /translation="MDFDFSDDQLALRDAVAKWVEKSYDFERRRAIVAAGGFDRAAYG
                     ELAELGLAGICVGEDHGGLGMGPVETMVVLEELGRGIVVEPLTQTFVVSTTLGAHGSD
                     AVRQAWLPRIASGEALVVLAHQERKARYRLDTCEAQASATGGGHTVTGLKHIVAAGDQ
                     ADAWLVPAQLNGKLALFLIERGAQGVNTRGYLTQDGSRAADVQFNGAPAQLVTTDGLT
                     ALMHAADVGIACACAYGVGAMDQTLKLTVDYMNQRKQFGVVIASFQALRHRVADMKMQ
                     LELARSMSYYATLKLGAPEAERRQALSRAKVQLGQSLRHVGQQSVQLHGGIGVTDEYV
                     GSHYFKSLTQLEMSWGDTLHHLGQVSQRMTDTAGVFA"
     gene            1562..>2422
                     /locus_tag="PK242_13485"
     CDS             1562..>2422
                     /locus_tag="PK242_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ95078.1"
                     /translation="MSPVLPPPSPAGGSPRLLLWLVAIGFFMQTLDSTIVNTALPAMA
                     RSLGESPLRMQAVVVSYALTMAAVIPATGWLADRFGTRRVFALAIALFTLGSAACAAA
                     PSLTLLTAARVLQGVGGAMLLPVGRLAVLQAFPRERFLEAMSFVAIPGLVGPLIGPTL
                     GGWLVEVASWHWIFLINLPVGLLGLLATRRHMPNLRAAQAGRFDLGGYLLLVLAMVSI
                     SLSLDGLGGLGWARTLVVLLMVLGLAALAAYWLHAQRSPAPLFAPALFGVASYRVGVL
                     GNLFARIGSGA"
BASE COUNT          349 a          870 c          836 g          367 t
ORIGIN      
        1 gacatcgtgg cgctggagat gctggtgctg cgcgtgctgt cggccgaaaa gtccggccgc
       61 aacgcgctcg acatcgccgg tctgctcaag atcaagggca gcgaaatcca gcagcgctac
      121 accgaactca tgatgctggc cgccggcccg ttcagcctgc cctacatctg ggaagccatg
      181 gaagccggct ggcagggcga ccaggctgcc gccgccgggc tgcagggctt ccccggcggc
      241 gccgtggcca acgccacgct ggccgccagc tacttcaact accgcaagac caccatctac
      301 ggcggctcca acgaagtgca gcgcaacatc gtcgcgcaga ccgtgctggg ctgaacagaa
      361 cacaaggagt cagagacatg gatttcgatt tttccgacga ccagctggcc ctgcgcgacg
      421 ccgtcgccaa gtgggtcgaa aagagctacg acttcgagcg ccgccgcgcc atcgtcgccg
      481 ccggcggctt tgaccgcgcc gcctacggcg aactggccga actgggcctg gccggcatct
      541 gcgtcggcga agaccacggc ggcctgggca tgggcccggt cgagaccatg gtggtgctgg
      601 aagagctggg ccgcggcatc gtggtcgagc cgctgacgca gaccttcgtg gtcagcacca
      661 ccctgggcgc ccacggcagc gacgccgtgc gccaggcctg gctgccgcgc atcgccagcg
      721 gcgaggcgct ggtggtgctg gcccatcagg agcgcaaggc acgctaccgg ctggacacct
      781 gcgaagccca ggccagcgcc accggcggcg gtcacaccgt caccggcctg aagcacatcg
      841 tggccgccgg cgaccaggcc gacgcctggc tggtgccggc ccagctgaac ggcaagctgg
      901 cgctgttcct gatcgagcgc ggcgcccagg gcgtgaacac gcgcggctac ctgacgcagg
      961 acggctcgcg cgccgccgac gtccagttca acggcgcgcc ggcgcaactg gtcaccaccg
     1021 acggcctgac cgccctgatg cacgccgccg acgtcggcat cgcctgcgcc tgcgcgtacg
     1081 gcgtgggtgc gatggaccag acgctcaagc tcacggtcga ctacatgaac cagcgcaagc
     1141 agttcggcgt ggtcatcgcc agcttccagg ccttgcgcca ccgcgtcgcc gacatgaaga
     1201 tgcagctgga gctggcgcgc agcatgagct actacgccac gctcaagctc ggcgcccccg
     1261 aggccgagcg ccgccaggcg ctgtcgcgcg ccaaggtaca gctgggccag tcgctgcgcc
     1321 atgtgggcca gcagtcggtg cagctgcacg gtggcatcgg cgtgaccgac gaatacgtgg
     1381 gcagccacta cttcaagagc ctgacccagc tggaaatgag ctggggcgac accctgcacc
     1441 acctgggcca agtcagccag cgcatgaccg acaccgccgg cgtgttcgcc tgaccgtccg
     1501 gcccagtccc cctcccccgc acaggcgctg ccggacaacg gcggcgcctt tttcgttcgc
     1561 gatgtcaccc gtcctaccgc ccccatcgcc cgccggcggc agcccacgcc tgctgctgtg
     1621 gctggtggcg atcggctttt tcatgcagac gttggactcg accatcgtca ataccgcgct
     1681 gccggccatg gcgcgcagcc tgggcgaaag cccgctgcgc atgcaggcgg tggtggtgag
     1741 ctatgcgctg acgatggcgg cggtgatccc ggccaccggc tggctggccg accgcttcgg
     1801 cacccggcgc gtgtttgcgc tggccatcgc cctgttcacc ctgggctcgg ccgcctgtgc
     1861 ggcggcgccc agcctgacgc tgctgaccgc cgcgcgcgtg ctgcagggcg tgggtggcgc
     1921 catgctgtta ccggtggggc ggctggcggt gctgcaggcg tttccgcgcg aacgcttcct
     1981 ggaggccatg agcttcgtcg ccatccccgg cctggtcggc ccactgatcg gtccgaccct
     2041 gggcggctgg ctggtggaag tcgccagctg gcactggatc ttcctcatca acctgccggt
     2101 cggcctgctg ggcctgctgg cgacccggcg ccacatgccc aacctgcgcg ccgcccaggc
     2161 cggccgcttc gacctgggcg gctacctgct gctggtgctg gcgatggtca gcatttcgct
     2221 gtcgctggac ggcctgggcg gcctgggctg ggcccgcacg ctggtggtgc tgctgatggt
     2281 gctggggctg gcggcgctgg cggcctactg gctgcacgcg cagcgcagcc cagcgccact
     2341 gttcgctccg gcgctgtttg gcgtggccag ctaccgcgtg ggcgtgctgg gcaacctgtt
     2401 cgcgcgcatc ggctcgggcg cc
//
LOCUS       DAOOOG010000290         2399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_54756_length_2399_cov_23.527730, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000290 DAOOOG010000000
VERSION     DAOOOG010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54756_length_2399_cov_23.527730"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..475
                     /locus_tag="PK242_13490"
     CDS             65..475
                     /locus_tag="PK242_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOZ95079.1"
                     /translation="MNELLSAINAVRATARSCGGTAMPAAAALSWNALLAHAAEAHST
                     DMATHNFFSHTGSNGSNMAQRVDAAGYVWQALAENIAAGNASVAATVSQWVNSPGHCQ
                     AMMSATYVHVGGACRYNASTQYGYYWTIDLGKPF"
     gene            complement(612..1268)
                     /gene="adk"
                     /locus_tag="PK242_13495"
     CDS             complement(612..1268)
                     /gene="adk"
                     /locus_tag="PK242_13495"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOZ95080.1"
                     /translation="MRLILLGAPGAGKGTQAAFICQKYGIPQISTGDMLRAAVKAGTP
                     LGLQAKGVMESGGLVSDELIINLVKERIAQPDCASGFLFDGFPRTIPQAEAMKAAGVK
                     LDYVLEIDVPFDAIIERMSGRRSHPASGRTYHVKFNPPKVEGKDDVTGEPLVQRDDDK
                     EETVRKRLDVYSQQTRPLVDYYGDWARREPAAAPKYRQISGTGTVEEITARALAALAS
                     "
     gene            complement(1638..>2399)
                     /gene="kdsB"
                     /locus_tag="PK242_13500"
     CDS             complement(1638..>2399)
                     /gene="kdsB"
                     /locus_tag="PK242_13500"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOZ95081.1"
                     /translation="FTVLIPARLASTRLPNKPLADIAGKPMIVRVAERAERSGAAQVV
                     VAADAPEILHACAAHGVRAIATRADHASGSDRLAEACALLGLDGDDVVVNVQGDEPLI
                     DPALIDAVAALLSSSPVARMGTAAHAIENVADFLNPNVVKVVTDAQGLALTFSRAPLP
                     WWRDGFAGGVTALPAQPAPLRHIGLYSYRAGFLRAFASLAPAPLEQCEALEQLRALWH
                     GHRIAVHVTAQAPGPGVDTPEDLARVRALLASGAG"
BASE COUNT          357 a          808 c          860 g          374 t
ORIGIN      
        1 gcctgcgcct gcgcctgcgc ctgcgccagc accagcacca tcaccggtca gtgcgtgcgg
       61 cgacatgaac gagttgctga gcgccatcaa tgccgtgcgc gccacggccc gcagctgtgg
      121 tgggacggcc atgccggctg cggcggcgct gagctggaac gcgctgttgg cgcatgccgc
      181 cgaggcgcac tcgaccgaca tggcgaccca caatttcttc agtcacacgg gctcgaacgg
      241 cagcaacatg gcgcagcggg tcgacgcggc tggctatgtc tggcaagccc tggcggaaaa
      301 cattgcggct ggcaacgcga gcgtcgcggc caccgtcagc caatgggtga acagccccgg
      361 ccactgtcag gccatgatga gcgccaccta cgtgcatgtg ggcggcgcct gccgctacaa
      421 cgccagcacc cagtacggct actactggac gatcgatctg ggcaagccgt tctgagcctc
      481 agagcggtga cgccgcacgc ccaggacgcg gcaaaaaaat gcggcgccag ggcgggcgcc
      541 gcttcggtga gtgcaggcgg tggctcagcg gacgtggatg gggctgcccc caggaacgcc
      601 aactcggcgc atcagctggc cagcgccgcc aaggcgcggg cggtgatttc ttccaccgtg
      661 ccggtgccgc tgatctggcg gtacttgggc gcggcggcgg gctcgcgccg ggcccagtcg
      721 ccgtagtagt cgaccagcgg gcgcgtctgc tggctgtaga cgtccaggcg cttgcgcacg
      781 gtttcttcct tgtcgtcgtc gcgctgcacc aacggttcgc cggtgacgtc gtccttgccc
      841 tcgaccttgg gcgggttgaa cttgacgtgg taggtgcggc ccgaggccgg gtggctgcgg
      901 cggccgctca tgcgctcgat gatggcgtcg aacggcacgt cgatctccag cacgtagtcc
      961 agcttgacgc cggcggcttt catggcctcg gcctgcggga tggtgcgcgg aaagccgtcg
     1021 aacagaaagc cgctcgcgca atcgggctgg gcgatgcgct ccttgaccag gttgatgatg
     1081 agttcgtcac tgaccaggcc gcccgactcc atcacgccct tggcctgcag gcccagcggt
     1141 gtgccggcct tgacggcggc gcgcagcatg tcgccggtgg agatctgcgg gatgccgtac
     1201 ttctggcaga tgaaggccgc ttgcgtgccc ttgccggcgc cgggggcgcc caacaggatc
     1261 agtctcatcg tgctcctctg tgtgtccgtg ggagggggaa ggccggcgcg catggccaca
     1321 aggggccggg ccggaacggg ttgcgttcga gaatagcacg cggggccttg tggccggctt
     1381 acagacaagg cgtgccggcc ttgggctcgc ctgatacacg aactcgcttt caacctggtc
     1441 caacatgatt catgacgctc gcccccggct gcgcttcgtc atgtcggcgc agccggggtc
     1501 acaggttctc ttcttgaacg ccaagccgta ggccgggtgc ccggccgtgc tcggggttgt
     1561 cctgccacat ccggcctcac gccttgtctg gcaagcgccg gcagctatgg tttgaatagc
     1621 gtagggagcg ggcgcgctca gcccgcaccg ctcgccagca gggcccgcac gcgcgccagg
     1681 tcctcgggcg tgtcgacgcc ggggccgggc gcctgggccg tcacatgcac ggcgatgcgg
     1741 tggccgtgcc agagcgcacg cagctgctcc agcgcctcgc attgctccag cggcgccggc
     1801 gccaggctgg cgaaggcccg cagaaagccg gcgcggtagc tgtacaggcc gatgtggcgc
     1861 agcggcgccg gctgcgccgg caaggccgtg acgccgccgg caaagccgtc gcgccaccag
     1921 ggcagcggcg cgcgcgagaa cgtgagcgcc agcccctggg cgtcggtcac caccttgacg
     1981 acgttcgggt tcaggaaatc agctacgttt tcgatagcgt gtgccgcagt ccccatgcga
     2041 gcgacaggcg aactgctcaa caaagcggcg acggcatcga tgagcgccgg gtcgatcagc
     2101 ggctcgtcgc cctgtacgtt gaccaccacg tcgtcgccgt ccagacccag cagtgcgcag
     2161 gcttcggcca ggcggtcgct gccactggcg tgatcggcgc gcgtggcgat ggcgcgcacg
     2221 ccgtgggcgg cgcaggcgtg caggatctcg ggcgcatcgg ccgccaccac cacctgcgcc
     2281 gcgcccgagc gctcggcgcg ctcggccacg cgcacgatca tcggcttgcc agcgatgtcg
     2341 gccagtggct tgttcggcag ccgggtcgag gccagccggg cggggatgag gacggtgaa
//
LOCUS       DAOOOG010000291         2397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_54805_length_2397_cov_24.793766, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000291 DAOOOG010000000
VERSION     DAOOOG010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54805_length_2397_cov_24.793766"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PK242_13505"
     CDS             complement(<1..884)
                     /locus_tag="PK242_13505"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOZ95082.1"
                     /translation="MKTLKRLLLALLLMLIVAAGIGWFSLDKEARGLLATFPTNRDIL
                     FWNEKQRDAAFRAMDRIALLAKWRTAAPSAQVRPLPPGPPLKLPLDVDAYLREQHGAA
                     LLILHKGQLRLERYGLGFDGSGRWTSFSVAKSFTSTLVGAALKDGHIKSLDDKVSAYI
                     PDMKGSAYDDVSIHQLLTMTSGIRWNEDYGDTNSDVARFNNHKPEPGVHALVSYLRQL
                     PRAAPPGTRWNYSTGETNLVGILISQAVGKPLTEYMQEKIWSRIGSEQQATWLLSKTG
                     QEISGCCIQAAPRDYARFG"
     gene            complement(932..1969)
                     /gene="cobT"
                     /locus_tag="PK242_13510"
     CDS             complement(932..1969)
                     /gene="cobT"
                     /locus_tag="PK242_13510"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOZ95083.1"
                     /translation="MSLNLPPVPDLHQPDLAAALRHKISRKTVPEGALGRMAALAERL
                     GLILGTAEPHLSAPQLLVCAADHGIAARGVSAYPSDVTWQMVGNFLAGGALVSVLARQ
                     HGIAQTVVDCGVRHDFAPAPGLVIRKIAPGTQDSSQGPAMSAAQCAQALANGIELVKA
                     LPGNALLLGEMGIGNTSAASLLVARLGDIDVAEVTGAGTGLDAAGIARKTAVLRQALA
                     ANAGATEPLDALAALGGFEIAILTGAVLQAASERRVIVVDGFIASSAVLVASRIAPAV
                     LQRCVFAHRSDERGHARLLDLLGADPLLALGLRVGEGSGAVLAWPLLESSCRILNEMA
                     SFESAGVSDKA"
     gene            complement(1993..>2397)
                     /locus_tag="PK242_13515"
     CDS             complement(1993..>2397)
                     /locus_tag="PK242_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HOZ95084.1"
                     /translation="TNGPGLGLLPLVTSFEPQKTVRRTRARFADVTGAWSPLSGVAVQ
                     GYEIHSGQTAQHPAMAAKGDVAREVIPGLAWQNPAGNVLGCYLHGLFEDAAVLHALFG
                     AQAPTLDAVFDGLAAELERAFAPGALQALIAR"
BASE COUNT          345 a          836 c          858 g          358 t
ORIGIN      
        1 ccaaagcgcg cgtagtcgcg cggcgcggcc tggatgcagc agccgctgat ctcctgcccg
       61 gtcttggaca aaagccaggt ggcctgctgc tcgctgccga tgcgcgacca gatcttttcc
      121 tgcatgtact cggtcagcgg cttgcccacc gcctggctga tcaggatgcc gaccagattc
      181 gtctcgccgg tgctgtagtt ccagcgcgtg cccggcggtg ctgcgcgcgg cagctggcgc
      241 agatagctga ccagcgcatg cacgcccggc tcgggcttgt ggttgttgaa gcgcgccacg
      301 tcggagttgg tgtcgccgta gtcctcgttc cagcggatgc ccgaggtcat ggtgagcagt
      361 tgatgaatgc tgacgtcgtc gtaggccgaa cccttcatat cggggatgta ggcgctgacc
      421 ttgtcgtcca ggcttttgat gtggccgtcc ttcagcgccg cgcccaccag ggtcgaggtg
      481 aaggacttgg cgaccgaaaa gctggtccag cgcccgctgc cgtcaaagcc caggccgtag
      541 cgctccagcc gcagctggcc cttgtgcagg atcagcaacg ccgcgccgtg ctgctcgcgc
      601 aggtaggcgt ccacgtccag cggcagcttg agcggcgggc cgggcggcag cggccgcacc
      661 tgcgcgctgg gcgcggcggt gcgccacttg gccagcagcg cgatgcggtc catggcgcga
      721 aaggcggcgt cgcgctgctt ctcgttccag aacaggatgt cgcggttggt cggaaaggtg
      781 gccagcaggc cgcgcgcctc cttgtccagg ctgaaccagc cgatgccggc ggccacgatc
      841 agcatcagca gcagcgccag cagcagtcgc ttgagggttt tcaacacagc tctccttggt
      901 tcgtcgtggc gcgcatggta gcgcccgccc ttcaggcctt gtccgacacg ccggccgatt
      961 cgaaactggc catctcgttg aggatgcggc agcttgactc cagcagcggc caggccagca
     1021 cggcgccgct gccctcgccc acgcgcaggc ccagcgccag cagcggatct gcacccagca
     1081 gatccagcag gcgggcatgg ccgcgctcgt cggagcggtg ggcgaacacg cagcgctgca
     1141 gcaccgccgg cgcgatgcgg ctggccacca gcaccgcgct gctggcgatg aagccatcga
     1201 ccacgatcac gcgccgctcg ctggcggcct gcagcacggc gccggtcagg atggcgatct
     1261 cgaagccgcc cagcgcggcc agcgcatcca ggggctcggt ggcgcccgcg ttggcggcca
     1321 gcgcctggcg cagcacggcg gtcttgcggg cgatgccggc ggcgtccagc ccggtgccgg
     1381 caccggtaac ctcggccacg tcgatgtcgc ccaggcgcgc caccagcagc gacgcggcgc
     1441 tggtgttgcc aatgcccatc tcgcccagca gcagggcgtt gccgggcagg gccttgacca
     1501 gctcgatgcc gttggccagg gcctgcgcgc actgcgcggc gctcatggcc ggcccttggc
     1561 tgctgtcctg cgtgccgggc gcgatcttgc ggatcaccag ccccggcgcg ggcgcgaagt
     1621 cgtggcgcac gccgcagtcc accaccgtct gcgcgatacc gtgctggcgc gccagcacgc
     1681 tgaccagcgc gccgccggcc agaaagttgc ccaccatctg ccaggtcacg tcgctcgggt
     1741 aggccgacac gccgcgcgcg gcgatgccgt ggtcggccgc gcacaccagc agttgcggcg
     1801 cgctgaggtg cggctcagcc gtgcccagga tcaggcccag ccgctcggcc agcgcggcca
     1861 tgcggccgag cgcgccctcg ggcacggtct tgcgactgat cttgtgacgc agggcggcgg
     1921 ccagatcggg ttgatgcaga tcggggacgg ggggcaggtt caggctcatg gggtgaagca
     1981 acaaacgaag attcagcgcg cgatcagcgc ctgcagcgca cccggcgcaa aggcgcgctc
     2041 cagttcggcg gccaggccgt cgaacaccgc gtccagcgtc ggcgcctggg cgccaaacag
     2101 cgcgtgcagc acggcggcgt cttcgaacag gccgtgcaga tagcagccca gcacgttgcc
     2161 ggccgggttc tgccaggcca ggccggggat gacctcgcgc gccacgtcgc ccttggccgc
     2221 catggccggg tgttgggcgg tctggccgct gtgaatttcg tagccctgca cggccacgcc
     2281 cgacagcggc gaccaggcgc cggtcacgtc ggcaaagcgg gcgcgggtgc ggcgcacggt
     2341 tttttgcggc tcgaagctgg tcaccagggg cagcaggccc aggccggggc cgttggt
//
LOCUS       DAOOOG010000292         2374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_55565_length_2374_cov_25.004312, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000292 DAOOOG010000000
VERSION     DAOOOG010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55565_length_2374_cov_25.004312"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /gene="xth"
                     /locus_tag="PK242_13520"
     CDS             <1..777
                     /gene="xth"
                     /locus_tag="PK242_13520"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOZ95085.1"
                     /translation="ISTWNVNSLTARLPHVLDWLAANPVDVLALQELKLTDDKFPHQA
                     LGEAGYAAVAFGQKTYNGVALLTRAPLAAQAVVRNIPGFADESARVIAATLEAPGGPL
                     RVVNGYFVNGQAPGSDKFDYKMSWLRALRDWLREELAAHPRLVLLGDFNIAPEDRDSY
                     DPVGLAETIHHTSEERHHFQALLGLGLADAFRLFEQPDKSFSWWDYRMLGYQKNRGLR
                     IDHILISQPLVAQATACHIDRVPRKWPKPSDHAPVTLELA"
     gene            complement(1214..1738)
                     /locus_tag="PK242_13525"
     CDS             complement(1214..1738)
                     /locus_tag="PK242_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95086.1"
                     /translation="MKPLLPIPWVPLLAAALAVAAWPAAAQDRIYRCGNEYTNQPKGR
                     SDCKLVEGGNVTIVRGTAPAAAPAATAPAGTAPAARSGGASPASAPRVDNAEQRKRDA
                     DAKAILQQELRRAEAKQAELEKEYKGGEPDKIGGEARNHQKYLDRVAELKAAIERNQS
                     DIEGIKRELGRLGP"
     gene            complement(1905..>2374)
                     /gene="glnA"
                     /locus_tag="PK242_13530"
     CDS             complement(1905..>2374)
                     /gene="glnA"
                     /locus_tag="PK242_13530"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427647.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOZ95087.1"
                     /translation="PTTNSYKRLVPHFEAPVKLAYSARNRSASIRIPHAASDKARRIE
                     ARFPDPMANPYLCFAALLMAGLDGIENKIHPGEAATKDLYHLPPEEDKLVPTVCHSLD
                     QALEELDRDRAFLTRGGVFTDAMLDAYIELKMTEVNRIRVQVNPAEYDLYFSL"
BASE COUNT          368 a          774 c          835 g          397 t
ORIGIN      
        1 atcagcacct ggaacgtcaa ctccctcacc gcccgcctgc cgcacgtgct ggactggctg
       61 gcggccaacc cggtcgacgt gctggcgctg caggagctga agctgacgga cgacaagttt
      121 ccgcatcagg cgctgggcga agccggctac gccgccgtgg ccttcgggca gaagacctac
      181 aacggcgtcg ccctgctgac gcgcgcgccg ctggcggcgc aggcggtggt gcgcaacatc
      241 cccggttttg ccgacgaatc ggcgcgcgtg atcgccgcca cgctggaggc gcccggcggg
      301 ccgctgcggg tggtcaacgg ctatttcgtc aacggccagg cgccgggctc ggacaagttc
      361 gactacaaga tgagctggct gcgcgcgctg cgcgactggc tgcgcgagga gctggcggcg
      421 cacccgcgcc tggtgctgct gggcgacttc aacatcgcgc cggaagaccg cgacagctac
      481 gacccggtgg gcctggccga aaccattcac cacaccagcg aggagcgcca ccacttccag
      541 gccctgctgg ggctgggcct ggcggacgcc ttccggctgt tcgagcagcc ggacaagagc
      601 ttcagctggt gggactaccg catgctgggc taccagaaaa accgcggctt gcgcatcgac
      661 cacatcctga tcagtcagcc gctggtggcg caggcgacgg cgtgccatat cgaccgcgtg
      721 ccgcgcaaat ggcccaagcc cagcgaccat gcgccggtga cgctcgaact ggcctgaagc
      781 gcacggccca tgaatgccac agcccaatcg ggctgtagcg cgctaccaat ctccgccaac
      841 agctattatt acaatagcaa acgagcggcg aagcgattca gcctaggagg ctgtcgggct
      901 tggagcgccg aaagcgagaa tcggccccag cgagaccagt ttttgccgga tttgcagccc
      961 aatagttgag ctattgggca aaaagccggt gaaaaatggg ccgctgcggt cgatttgcag
     1021 ccggcgattt ccaagtccga cagcctccta ggttcaggcc acgcggcatc ggccctgcga
     1081 tacggggtac cctgcatggg gacgggatgc cgcacgccaa gcgctgacac ggccgcagcc
     1141 gtacccggca ttggctgcgc agaccgctcc gggccgggag gcgccgcgcg ccagggggtt
     1201 gttcagcgtc gcctcaaggt ccaaggcggc ccagctcgcg cttgatgcct tcgatgtcgc
     1261 tctggttgcg ctcgatcgcg gccttcagtt cggccacgcg gtcgaggtac ttctggtggt
     1321 tgcgcgcttc gccgccgatc ttgtcgggct caccaccctt gtactccttc tccagctcgg
     1381 cttgcttggc ctcggcgcgg cgcaactcct gctgcaggat ggccttggcg tcggcgtcgc
     1441 gcttgcgctg ctcggcgttg tcgacgcgcg gcgccgaggc gggtgaagcg ccgcccgagc
     1501 gcgccgccgg ggccgtcccg gcgggggctg tggccgccgg cgccgccgcg ggtgcggtgc
     1561 cgcgcacgat ggtgacgttg ccgccttcca ccagcttgca gtcggagcgg cccttgggtt
     1621 ggttggtgta ctcgttgccg cagcggtaga tgcggtcctg cgccgccgca ggccacgccg
     1681 cgacggccag cgcggcggcg agcaacggca cccaggggat cggcagcaga ggtttcatgt
     1741 cggtcggtac aacggccccg gcaacgcgcc gggatcagcc ttcaacgatc aagtatcggg
     1801 caggtggacc gctttggggc aggcttgttc ccgaaaatcg ccactgaacc ggcccaggcc
     1861 gccgttgggc ggcccatttc gtgtcattgt ggcgacaagc gcggtcagag cgagaagtac
     1921 aggtcgtact cggccgggtt gacctgcacg cggatgcggt tgacctcggt catcttcagc
     1981 tcgatgtagg cgtcgagcat ggcgtcggtg aacacgccgc cgcgcgtcag gaaggcgcgg
     2041 tcgcgatcca gctcttccag cgcctggtcc aggctgtggc acaccgtggg caccagcttg
     2101 tcttcttcgg gcggcaggtg gtagaggtcc ttggtggcgg cttcacccgg gtggatcttg
     2161 ttctcgatgc cgtccaggcc ggccatcagc aacgccgcaa agcacagata ggggttggcc
     2221 atggggtcgg gaaagcgcgc ttcgatgcgg cgcgccttgt cgctggcggc gtgcgggatg
     2281 cggatcgagg ccgagcggtt gcgcgccgag taggccagtt tgaccggcgc ctcgaagtgc
     2341 ggcaccaggc gcttgtagct gttggtggtg gggt
//
LOCUS       DAOOOG010000293         2360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_56011_length_2360_cov_24.556616, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000293 DAOOOG010000000
VERSION     DAOOOG010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56011_length_2360_cov_24.556616"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PK242_13535"
     CDS             complement(<1..207)
                     /locus_tag="PK242_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOZ95088.1"
                     /translation="MDPKLLELLVCPVTKGPLDHDRDKGELISRSARLAYPIRDGIPI
                     MLEAEARALSDDELAALPPRTPLVG"
     gene            complement(218..550)
                     /locus_tag="PK242_13540"
     CDS             complement(218..550)
                     /locus_tag="PK242_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HOZ95089.1"
                     /translation="MKNHLFLLGAIVAEVVATSALKASDGLSRGGPTALVVIGYALAF
                     WLLSLTLRTIPVGIAYATWSGLGMVLIALAGWWLYGQRLDGWALLGMALVVAGVLVMN
                     LLSPSSAH"
     gene            complement(547..1656)
                     /gene="lpxK"
                     /locus_tag="PK242_13545"
     CDS             complement(547..1656)
                     /gene="lpxK"
                     /locus_tag="PK242_13545"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOZ95090.1"
                     /translation="MLRALIERRLRAAWQRRGVLARLLWPLSRLYGALAAHRRRRYLS
                     GALPVARLPVPVIVVGNVIVGGAGKTPTTLALVAHLQARGWRPGIVSRGHGRASDDCR
                     AVQPDSDPREVGDEPLLLRRRAGVPVFVARQRAQAGRALLAAHPDTDVLVCDDGLQHL
                     ALARDLEVVVFDARGCGNHWLLPAGPLREPWPRSPAAAREPWPGSPVDPREPGPRPAE
                     LVLWTEPPAPDQAPAPAAFGATRALAEHALRADGGRPPLADLRGQPLTALAAIARPEA
                     FFAMLRAAGLTLTDTLALPDHYDFDSCSRLLDGRQNLICTEKDAVKLWRHRPDALAVP
                     LRLQVPAAFFAALDERLAARGYHPASRADRPTAAP"
     gene            complement(1874..2284)
                     /locus_tag="PK242_13550"
     CDS             complement(1874..2284)
                     /locus_tag="PK242_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOZ95091.1"
                     /translation="MKFRPHPPEEPEVNLIPFIDVLLVILIFLMLTTTYSKFTELQVT
                     LPVADADASRERPKEIVVAVAADGRYAVNRQPLEARSVDAVARALRALAGKDSVLIIS
                     ADATAPVQAVVTVMDAARREGLTQITFAAQSGGR"
BASE COUNT          330 a          834 c          919 g          277 t
ORIGIN      
        1 gcccaccagc ggcgtgcgcg gtggcagcgc ggccagttcg tcgtcgctga gggcgcgcgc
       61 ctcggcttcc agcatgatgg ggatgccatc gcggatcggg taggccaggc gggccgagcg
      121 tgagatcagc tcgcccttgt cgcggtcgtg gtccagcggg cctttggtga cggggcagac
      181 cagcagttcg agcagcttgg ggtccatggt gatccgatca gtgggcagag gagggagaca
      241 gcaggttcat gaccagcacc ccggccacga ccagcgccat gcccagcagc gcccagccgt
      301 ccagccgctg gccatacagc caccagccgg ccagggcgat cagcaccatg cccaggcccg
      361 accaggtggc gtaggcgatg ccgaccggga tggtgcgcag cgtcagcgac agcagccaga
      421 acgccagcgc atagccgatc accaccagcg ccgtcggccc gccgcgcgac aggccgtcgc
      481 tggccttgag cgcgctggtg gcgacgactt cggcgacgat ggcgcccagc aggaacaggt
      541 ggtttttcat ggcgcggcgg tgggccggtc ggcccggctg gcgggatgat agccgcgcgc
      601 ggccagccgc tcgtcgagcg cggcgaagaa cgctgccggc acctgcaggc gcagcggcac
      661 ggccagcgcg tcggggcggt ggcgccacag tttgaccgcg tctttttcgg tgcaaatcag
      721 gttctggcgc ccgtccagca agcgcgagca actatcaaaa tcgtagtgat cgggcagggc
      781 cagggtgtcg gtcagggtca ggccggcggc gcgcagcatg gcaaagaagg cctcgggccg
      841 tgcgatggcg gccagcgcgg tcagcggctg gccgcgcaga tcggccagcg gcggcctacc
      901 gccatcggca cgcagggcgt gctcggccag cgcgcgggtg gcgccgaagg cggctggtgc
      961 gggggcctgg tcgggggcgg gcggctcggt ccacagcacc agctcggccg ggcgcggccc
     1021 gggttcgcgc ggatcgacag ggctgcccgg ccagggctcg cgcgcagcgg cagggctgcg
     1081 cggccacggc tcacgcaggg gcccggccgg cagcagccag tggttgccgc agccgcgggc
     1141 gtcgaacacc accacctcca ggtcgcgcgc cagggccagg tgctgcagcc cgtcgtcgca
     1201 gaccaggacg tcggtgtccg ggtgcgccgc cagcagggcg cggccggcct gagcgcgctg
     1261 gcgcgccacg aacaccggca cgccggcgcg ccggcgcagc agcagcggct cgtcgccgac
     1321 ctcgcgcggg tcgctgtcgg gctgcacggc gcggcagtcg tcgctggcgc ggccgtggcc
     1381 gcgcgagacg atgccgggcc gccagccgcg cgcctgcagg tgcgccacca gggccagcgt
     1441 ggtcggggtc ttgccggcgc cgccgacgat cacgttgccg accacgatca ccggcaccgg
     1501 cagccgcgcc acgggcaggg cgccgctcag gtagcgccgg cgccggtgcg ccgccagcgc
     1561 cccgtacagc cgcgacagtg gccacagcag gcgcgccagc accccgcgcc gctgccaggc
     1621 ggcgcgcagg cggcgttcga tgagggcgcg cagcatcggg tcagcggttg accacgggcc
     1681 ggccaggccc gcagggcgcg ccgccacgca cccgcgcgag gcgccgcacg ctgccatggc
     1741 aatgccgggc gacgcagagc ggcgatgttc gggtgtgcaa agcgggcaag ggtggccttc
     1801 tttcaagctc gcggcgagcg gcggctgtgc gcagcgcgcg gcggcaggcg cagcatagcg
     1861 cccgcagggg ccgtcacctg cccccgctct gggcggcaaa ggtgatctgc gtcaggccct
     1921 cgcggcgcgc cgcgtccatc accgtcacca ccgcctgcac cggggcggtg gcgtcggcgc
     1981 tgatgatgag cacgctgtcc ttgccggcca gggcacgcag ggcacgcgcc acggcgtcga
     2041 cgctgcgggc ttccagcggc tggcggttga cggcgtagcg gccgtcggcg gcgaccgcca
     2101 ccacgatctc cttgggccgt tcgcgcgagg cgtcggcgtc ggccaccggc agcgtcacct
     2161 gcagctcggt gaacttgctg taggtggtgg tgagcatcag aaagatcagg atcaccagca
     2221 gcacgtcgat gaacgggatc aggttgacct ccggctcctc gggcgggtgc gggcggaact
     2281 tcatcgccca gctccggtat ccgcagcgcg gccggggcac agcggctcca ggtggcgcgc
     2341 aaagcgctcg gcggccagct
//
LOCUS       DAOOOG010000294         2358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_56074_length_2358_cov_23.645245, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000294 DAOOOG010000000
VERSION     DAOOOG010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56074_length_2358_cov_23.645245"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PK242_13555"
     CDS             complement(<1..856)
                     /locus_tag="PK242_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ95092.1"
                     /translation="MNTTTKTFPLEPIEKASIDELRALQLQRLRATLAHAYAHSPVYK
                     AKFDAAGVHPDDCRSLEDLARFPFTTKQDLRDHYPFGMFAVPREQLVRIHASSGTTGK
                     PTVVGYTRKDIDTWADVVARSFRAAGARPGDMLHQSYGYGLFTGGMGAHYGAERLGLT
                     VVPFGGGQTEKQIQLIRDFKPDIITVTPSYMLAICEQVEHMGLDPKELSLRIGIFGAE
                     PWTNEMRQAIEKRMAIDAVDIYGLSEVMGPGVANECVETKDGPTIWEDHFYPEIIDPE
                     TGAVLPDGQ"
     gene            complement(853..1320)
                     /gene="paaI"
                     /locus_tag="PK242_13560"
     CDS             complement(853..1320)
                     /gene="paaI"
                     /locus_tag="PK242_13560"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HOZ95093.1"
                     /translation="MRHNPAAQALAQRVGQAMFAADRASRETMGMELLACEPGRALMR
                     MTVQPLHLNGHQICHGGFIFTLADSTFAFACNSRNHNTVAAGCSIEFLKPAHAGDVLT
                     CEGVEQVQSGRHGVYDMRVTNQHGDVVALFRGKSAQIKGHVLADEPTSQESGA"
     gene            complement(1323..2126)
                     /gene="paaG"
                     /locus_tag="PK242_13565"
     CDS             complement(1323..2126)
                     /gene="paaG"
                     /locus_tag="PK242_13565"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HOZ95094.1"
                     /translation="MTQPTEPDVLYAEEGAVAILTLNRPASLNSFTRQMHRDLWAALD
                     RLEANPAIRALVLTGAGRGFCAGADLSEFDFADGPNLQQRADPGPVIERAFNPTTRRL
                     INVRVPTVCAVNGVAAGAGASVAMACDITIAAPGASFIQAFSKIGLVPDSGGTWLLPQ
                     RVGLARAMALCLTGDKLSAQDAKAMGMIWDVADDALAAAKALAGRLAAMPTRALVATR
                     HLLRGAHTHSLDEQLDLERDVQSRLGFTHDYIEGVMAFLQKRPAQFRGE"
     gene            2253..>2358
                     /locus_tag="PK242_13570"
     CDS             2253..>2358
                     /locus_tag="PK242_13570"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574703.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOZ95095.1"
                     /translation="MTYELITTRVEGGQVGVITLNRPKQLNALNSQLMV"
BASE COUNT          353 a          760 c          834 g          411 t
ORIGIN      
        1 cctgcccgtc gggcagcacg gcgccggtct cggggtcgat gatctcgggg tagaaatggt
       61 cttcccagat ggtggggccg tccttggttt cgacgcactc gttggccacg ccggggccca
      121 tcacctcgga caggccgtag atgtccaccg cgtcgatcgc catgcgcttt tcgatggcct
      181 ggcgcatttc gttggtccag ggttcggcgc cgaagatgcc gatgcgcaag ctgagctcct
      241 tcgggtccag ccccatgtgc tcgacctgtt cgcagatcgc cagcatgtag ctgggcgtga
      301 cggtgatgat gtcgggcttg aagtcgcgga tcagctgaat ctgcttttcg gtctggccgc
      361 cgccgaaggg caccaccgtc aggcccaggc gctcggcacc gtagtgcgcg cccatgccgc
      421 cggtgaacag gccgtagccg tagctctggt gcagcatgtc gccggggcgc gcgccggcgg
      481 cgcgaaacga gcgcgccacc acgtcggccc aggtgtcgat gtccttgcgg gtgtagccga
      541 ccacggtggg tttgccggtg gtgccgctgg aggcatggat gcgcacgagc tgctcgcgcg
      601 gtacggcgaa catgccgaac gggtagtggt cgcgcaagtc ctgtttggtg gtgaacggga
      661 accgggccag atcctccagc gaccggcagt cgtcggggtg cacgccggcg gcgtcgaact
      721 tggccttgta gaccggcgaa tgcgcgtagg cgtgcgccag cgtggcccgc agccgctgca
      781 gttgcagcgc acgcaactcg tcgatgctgg ccttttcgat cggctccagc gggaaggtct
      841 tggtggtggt gttcatgcac cgctctcctg gctcgtgggt tcgtcggcca gcacatggcc
      901 cttgatctgc gcgctcttgc cgcgaaacag cgccaccaca tcgccgtgct ggttggtcac
      961 gcgcatgtcg tagacgccgt ggcggccgct ttgcacctgc tcgacgccct cgcaggtgag
     1021 cacgtcgccg gcgtgcgcag gcttgagaaa ctcgatgctg caaccggccg ccacggtgtt
     1081 gtggttgcgg ctgttgcagg caaaggcaaa agtcgaatcg gccaacgtga atatgaaacc
     1141 gccgtggcag atctggtggc cgttcagatg cagcggctgc accgtcatgc gcatcagggc
     1201 acgacccggc tcgcaggcca gcagctccat gcccatggtt tcgcgcgagg cgcggtcggc
     1261 agcgaacatg gcctggccga cgcgttgcgc cagcgcctgg gccgcagggt tgtggcgcat
     1321 gttcattcgc ccctgaactg cgccgggcgc ttttgcagaa aggccatcac gccctcgatg
     1381 tagtcgtgcg tgaagcccag gcgcgactgc acgtcgcgct ccagatcgag ctgctcgtcc
     1441 agcgaatggg tgtgggcgcc gcgcagcagg tggcgcgtgg ccaccagcgc gcgcgtgggc
     1501 atggcggcca ggcgtccggc cagcgccttg gcggcggcca gcgcatcgtc ggccacgtcc
     1561 cagatcatgc ccatggcctt ggcgtcctgg gcgctcagct tgtcgcccgt caggcacagc
     1621 gccatggcgc gcgccagacc gacgcgctgc ggcagcaacc aggtgccgcc cgaatcgggc
     1681 accaggccga tcttgctgaa ggcctggatg aagctggcgc cgggcgcggc gatggtgatg
     1741 tcgcaggcca tggccaccga ggcgcccgcg cccgctgcca cgccgttgac cgcgcacacg
     1801 gtgggcacgc gcacattgat caggcgccgc gtggtggggt tgaaggcgcg ctcaatcacc
     1861 gggcctgggt cggcgcgctg ctgcaggttg gggccatcgg caaagtcgaa ctccgacagg
     1921 tcggcaccgg cgcagaagcc acgcccggcg ccggtcagca ccagggcgcg gatcgcgggg
     1981 ttggcctcca ggcggtcgag cgcggcccac aggtcgcggt gcatctggcg cgtgaagctg
     2041 ttgagtgagg cggggcggtt gagcgtgagg atggctacgg cgccttcctc cgcatagagc
     2101 acgtcgggct cggtcggctg ggtcatgggt tgtctccttg ggtcgcgaat ttaccattga
     2161 ccgactgtgc ggtcggctaa cctgcacagc agccgtcgtc gctggttata gtgcctcttg
     2221 catcgcttca cacgacctgt tcaggagaac ccatgactta cgaactcatc accacccgcg
     2281 tcgaaggcgg ccaggtgggc gtgatcacgc tcaaccggcc caagcagctg aacgcgctca
     2341 acagccagct gatggtcg
//
LOCUS       DAOOOG010000295         2339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_56732_length_2339_cov_28.606830, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000295 DAOOOG010000000
VERSION     DAOOOG010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56732_length_2339_cov_28.606830"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /gene="uvrB"
                     /locus_tag="PK242_13575"
     CDS             complement(<1..1800)
                     /gene="uvrB"
                     /locus_tag="PK242_13575"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOZ95096.1"
                     /translation="MSASVQAVQTPSPAGQFVSFPDSPFQLFQPYPPAGDQPTAIAQL
                     VEGVRDGEAFQTLLGVTGSGKTFTMANVIARLGRPAIVFAPNKTLAAQLYSEFREFFP
                     KNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSAINEHIEQMRLSATKSVLERRDTVIVA
                     TVSAIYGIGAPEDYLQMRMILKVGGKQGQREAIQQLVRMQYQRQDIDFARGTFRVRGD
                     TIDVFPAEHSELAVRIELFDDEVESISLFDPLTGHIQQKVPRFVIYPSSHYVTPREKT
                     LSAVESIKLELAERLKFFVGEGKLVEAQRLEQRTRFDLEMLAELGHCKGIENYTRHLS
                     GAAPGEPPATLTDYLPRDALMFLDESHQMIGQLNAMYNGDRSRKTTLVEYGFRLPSAL
                     DNRPLKFAEFERRMHQVIFVSATPADYENQHAGQVVEQVVRPTGLVDPEVEVRPATHQ
                     VDDVLQEIRIRVQQHERVLITTLTKRMAEQLTEYLTDNGVKVRYLHSDVDTVERVEII
                     RDLRLGAFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQTIGRAARNVN
                     GKAILYADRITDSMKKAMGETERRRAKQIAFNAE"
     gene            1903..>2339
                     /locus_tag="PK242_13580"
     CDS             1903..>2339
                     /locus_tag="PK242_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ95097.1"
                     /translation="MSLFSSVELAPRDPILGLNEQFAADANPNKVNLGVGVYFDENGK
                     LPLLACVQAAENAMMATPKPRGYLPIDGIAAYDDGVKKLVFGADSEPYQSGRIATVQG
                     LGGTGALKIGADFLKHLNPAARVLISDPSWENHRALFTQAGFEV"
BASE COUNT          372 a          768 c          774 g          425 t
ORIGIN      
        1 ctcggcgttg aaggcgatct gcttggcgcg gcggcgctcg gtctcgccca tggccttctt
       61 catcgaatcg gtgatgcggt cggcgtacag gatggccttg ccgttgacgt tgcgcgcggc
      121 gcggccgatg gtctggatca ggctgcgctc ggcgcgcaga aagccctcct tgtcggcgtc
      181 cagaatcgcc accagcgaga cctcgggaat gtccaggcct tcgcgcagca ggttgatgcc
      241 caccagcacg tcgaaggcgc ccaggcgcag gtcgcggatg atctccacgc gctcgacggt
      301 gtccacatcg ctgtgcaggt agcgcacctt gacgccgttg tcggtcaggt actcggtcag
      361 ctgctcggcc atgcgcttgg tgagcgtcgt gatgagcacg cgctcgtgct gctgcacgcg
      421 gatgcgtatt tcctgcagca cgtcatcgac ctgatgggtg gccgggcgca cctcgacctc
      481 ggggtcgacc aggccggtgg ggcgcaccac ctgctcgacc acctggccgg cgtgctggtt
      541 ctcgtaatcg gccggcgtgg cgctgacgaa gatcacctgg tgcatgcgcc gctcgaactc
      601 ggcgaacttg agcgggcggt tgtccagcgc gctgggcagg cgaaagccgt attccaccag
      661 ggtggtcttg cgcgagcggt cgccgttgta catggcgttg agctggccga tcatctggtg
      721 gctttcgtcc aggaacatga gggcgtcgcg cggcaggtag tcggtcagcg tggccggtgg
      781 ctcgcccggc gcggcgcccg acaggtggcg ggtgtagttc tcgatgccct tgcagtggcc
      841 cagctcggcc agcatctcca gatcgaaccg ggtgcgctgc tccagccgct gggcctcgac
      901 cagcttgcct tcgccaacga agaatttcag gcgctccgcc agttccagct tgatcgactc
      961 cacggccgac aaggtctttt cgcgcggcgt gacgtagtgg ctgctggggt agatgacgaa
     1021 gcgcggcact ttctgctgga tgtggcccgt gagcgggtcg aacaggctga tggactcaac
     1081 ctcgtcgtcg aacagctcga tgcgcaccgc cagctccgaa tgctcggccg ggaatacgtc
     1141 gatggtgtcg ccgcgcacgc gaaaggtgcc gcgcgcaaag tcgatgtcct ggcgctggta
     1201 ctgcatgcgc accagctgct gtatcgcctc gcgctggccc tgcttgccgc ccaccttcag
     1261 gatcatccgc atctgcaggt agtcctcggg cgcgccgatg ccgtagatgg cgctgacggt
     1321 ggccacgatc acggtgtcgc gccgctccag cacgctcttg gtggccgaca ggcgcatctg
     1381 ctcgatgtgc tcgttgatgg cgctgtcctt ctcgatgaac aggtcgcgct gcggcacata
     1441 ggcctcgggc tggtagtagt cgtagtagct gacgaagtac tcgaccgcgt tcttggggaa
     1501 gaactcgcga aactcgctgt agagctgcgc cgccagcgtc ttgttgggcg cgaacacgat
     1561 ggccgggcgg cccagccgcg cgatcacgtt ggccatggtg aaggtcttgc ccgagccggt
     1621 gacgcccagc agggtctgga aggcctcgcc gtcgcgcacc ccttccacca gctgcgcgat
     1681 ggccgtcggc tggtcaccgg cggggggata aggctgaaac agctggaacg gcgaatcggg
     1741 aaaactgacg aactgccccg ccggactggg cgtctgcacg gcttgcacac tggcactcat
     1801 gggaaaaccc tgtggccctt aaaatttcgg gcgaaccggc aagcctaccc gagccggcac
     1861 gtctttgcca cccggtggca cttcttccca aggacttcct tcatgtcgct gttttcttcc
     1921 gtcgaactgg ccccgcgcga ccccatcctg ggcctgaacg aacaatttgc cgccgacgcc
     1981 aacccgaaca aggtcaacct gggcgtgggc gtgtacttcg acgaaaacgg caagctgccg
     2041 ctgctggcct gcgtgcaggc ggccgaaaat gccatgatgg ccacacccaa gccgcgcggc
     2101 tacctgccca tcgacggcat cgccgcctac gacgacggcg tgaaaaagct ggtgttcggc
     2161 gccgattccg agccctacca gtccggccgc atcgccaccg tgcagggcct gggcggcacc
     2221 ggcgcgctga agatcggcgc ggacttcctc aagcacctca accccgccgc ccgggtgctg
     2281 atcagcgacc cgagctggga aaaccaccgc gccctgttca cccaggccgg cttcgaggt
//
LOCUS       DAOOOG010000296         2309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_57821_length_2309_cov_27.470275, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000296 DAOOOG010000000
VERSION     DAOOOG010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57821_length_2309_cov_27.470275"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK242_13585"
     CDS             complement(<1..566)
                     /locus_tag="PK242_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ95098.1"
                     /translation="MSAPTSPQRPAVLVARAIFPDIVEQLSRHFEVESNPDDTLWPRA
                     ELVRRLQGKQGVFTTGGERIDAELLAACPGLRICANMAVGYNNFDLPAMTAAGVLATN
                     APGVLTETTADMGFALLMAAARRVTEGEHFLRAGAWDRWAYDMLIGQDVHGSTLGIIG
                     MGRIGQAIARRGALGFGMRVIYHNRSRLP"
     gene            complement(643..1596)
                     /locus_tag="PK242_13590"
     CDS             complement(643..1596)
                     /locus_tag="PK242_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOZ95099.1"
                     /translation="MNPISLPANDLILFAHIVAAGSFTRAADRTGLPKATLSRRLSEL
                     EDALGERLLQRSTRRLALTEFGQHMLEHAQRLLEESAAATALAQHRQLTPQGTLRASF
                     PPEFSALSLVKVVSEFARRHPEVRLELDLSARRVDLVAERFDLAVRAATQLPDDSTLV
                     ARHIVTHEHGLFASPDYLRRHGTPATPAELERHVGLAMLGSGGERQPWRLTRGKERWE
                     GLPAQVHCANSVGLQQALAVQGLGIVGLSALFVRQSRDADSLQRVLPDWCLPPTGVWC
                     VTPGRRLLPKRTSAFIQILTEVLAEGGAAPAPLAAGHRPRR"
     gene            1726..>2309
                     /gene="wrbA"
                     /locus_tag="PK242_13595"
     CDS             1726..>2309
                     /gene="wrbA"
                     /locus_tag="PK242_13595"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HOZ95100.1"
                     /translation="MSVRLAIVYYSTYGTNHQMAEIAAEAARAAGAEVRLLKVPETAP
                     EAVVAGQPAWKAQADKTAQLPQATAADMEWANAYLIVAPTRFGIMASQMRAFIDTLGV
                     VWAKGGLANKAVSAMTSAQNAHGGQESTLLSFYATIMHWGGIVVAPGFTDPVIFKVGG
                     NPYGYSHTQGQPFSGDVKAAIGHQARRLVQFAAKL"
BASE COUNT          341 a          833 c          812 g          323 t
ORIGIN      
        1 ggcaggcgcg agcggttgtg gtagatcacc cgcatgccga agcccagcgc gccgcgccgc
       61 gcgatcgcct ggccgatgcg gcccatgccg atgatgccca gcgtgctgcc gtgcacgtcc
      121 tgcccgatca gcatgtcgta ggcccagcgg tcccaggcgc cggcgcgcag aaagtgttcg
      181 ccctcggtga cgcggcgcgc cgccgccatc agcagggcaa aacccatgtc ggcggtggtt
      241 tcggtcagca cccccggcgc gttggtggcc agcacgccgg cggccgtcat ggccggcagg
      301 tcgaagttgt tgtagcccac cgccatgttg gcgcagatcc tcagccccgg gcaggcggcc
      361 agcagctcgg cgtcgatgcg ctcgccgccg gtggtgaaca cgccctgctt gccctgcagg
      421 cggcgcacca gctcggcgcg cggccacagg gtgtcgtcgg ggttggactc gacctcgaag
      481 tggcgcgaca gctgctcgac gatgtcggga aagatcgccc gcgccaccag cacggcggga
      541 cgttggggtg aggtgggtgc gctcatgcca ccgagtgtag ggccagcccc gccggtggtg
      601 ccggcggcgc ggcgccgccc ccacgcccgg caggcccggc gctcatcggc gcggtcggtg
      661 accggcggcc agcggggcgg gcgcggcgcc gccctcggcc agcacctcgg tcaggatctg
      721 gatgaaggcg ctggtgcgct tgggcagcag gcgccgcccc ggcgtcacgc accacacccc
      781 ggtgggcggc aggcaccagt cgggcagcac gcgctgcagc gagtcggcgt cgcggctctg
      841 gcgcacgaac agcgccgaca gacccacgat gcccaggccc tggaccgcca gcgcctgttg
      901 cagccccacc gagttggcgc agtgcacctg ggccggcagg ccttcccagc gttccttgcc
      961 gcgcgtcagg cgccagggct ggcgctcgcc gccgctgccc agcatggcca gcccgacgtg
     1021 ccgctccagc tcggccggcg tggccggcgt gccatggcgg cgcaggtagt ccgggctggc
     1081 gaacaggccg tgttcgtgcg tcacgatgtg gcgcgccacc agggtgctgt cgtcgggcaa
     1141 ctgggtggcg gcgcgcaccg ccaggtcgaa gcgttcggcc accagatcga cgcgccgcgc
     1201 cgacaggtcc agctccagcc gcacctcggg gtggcggcgc gcgaactcgc tcaccacctt
     1261 caccagcgac agcgcgctga actcgggcgg aaacgaggcc cgcagcgtgc cctgcggcgt
     1321 cagctgccgg tgctgcgcca gcgccgtggc ggcggccgac tcctccagca gacgctgcgc
     1381 gtgctccagc atgtgctggc cgaactcggt cagcgccagg cggcgcgtgc tgcgctgcag
     1441 caggcgctcg cccagggcgt cttccagctc ggacaggcga cgcgacaggg tggccttggg
     1501 caggccggtg cggtcggcag cgcgcgtgaa gctgcccgcc gccacgatgt gggcaaacag
     1561 gatcaggtcg ttggcaggca ggctgatggg gttcatgggt gcacccattg ttccaatcat
     1621 ggaattgtat gagttggatt tgtgtattta tggccccaaa ggatcccaat agactggagg
     1681 ctgacacccc ctcgatcctc tccaaaccgt ttcacaggag ccctcatgag cgttcgtctg
     1741 gccatcgttt actactccac ctacggcacc aaccaccaga tggctgaaat cgccgccgag
     1801 gccgcccgcg ccgccggcgc cgaggtgcgc ctgctgaagg tgcccgaaac cgcccccgaa
     1861 gccgtggtgg ccggccagcc ggcctggaag gcgcaggccg acaagaccgc gcagctgccg
     1921 caggccacgg ccgccgacat ggagtgggcc aacgcctacc tgatcgtcgc gccgacgcgc
     1981 tttggcatca tggccagcca gatgcgcgcc ttcatcgaca ccctgggcgt cgtctgggcc
     2041 aagggtggtc tggccaacaa ggcggtgtcg gccatgacgt cggcgcaaaa cgcccacggc
     2101 ggtcaggaat ccaccctgct gtccttctac gccaccatca tgcactgggg cggcatcgtg
     2161 gtggcgcccg gcttcaccga cccggtgatc ttcaaggtcg gcggcaaccc ctacggctac
     2221 agccacaccc agggccagcc gttcagcggc gacgtcaagg ccgccatcgg tcaccaggcg
     2281 cgccggctgg tccagttcgc cgccaaact
//
LOCUS       DAOOOG010000297         2302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_58089_length_2302_cov_25.536716, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000297 DAOOOG010000000
VERSION     DAOOOG010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58089_length_2302_cov_25.536716"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PK242_13600"
     CDS             complement(<1..463)
                     /locus_tag="PK242_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOZ95101.1"
                     /translation="MSTPPLSDRDGKIWLDGQLVDWRDAKIHVLTHTLHYGCGAFEGV
                     RAYDTVGGTAVFRLQEHTQRLFNSAKILRMAIPFTPEQVNEAQKEVVRANQLKSCYLR
                     PLVWIGSEKLGVSPKGNRIHVMVAAWAWGAYLGEEGLKRGIRVKTSSYTRHH"
     gene            complement(631..1056)
                     /locus_tag="PK242_13605"
     CDS             complement(631..1056)
                     /locus_tag="PK242_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOZ95102.1"
                     /translation="MKWQRLWVMLGAVALGAGAWRAGGWAGLALLGSGLVLWFLLHYH
                     RLLTVMKRANERPIGHIGSAVMLHAKLRQGLPLLHVMALTQSLGERLSAPEAQPEVYR
                     WSDPGQSHVVTEFEAGRLVRWRLERPADPDVQASTPEAH"
     gene            complement(1190..>2302)
                     /gene="radA"
                     /locus_tag="PK242_13610"
     CDS             complement(1190..>2302)
                     /gene="radA"
                     /locus_tag="PK242_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOZ95103.1"
                     /translation="GGRDPSTAGPPQGASAPSGGSATGAAAQRGGMLYVTGEESGAQV
                     ALRARRLGIAHSQVAVLAEIQLEKILATLSAQQPAVAVIDSIQTVYSDQLTSAPGSVA
                     QVRECAAHLTRWAKASGCAVVLVGHVTKDGQLAGPRVLEHMVDTVLYFEGDTHSSYRL
                     IRAIKNRFGAVNEIGVFAMTERGLKGVSNPSAIFLAQHAEPVPGSCVLVTLEGTRPLL
                     VEVQALVDSGGPSPRRLSVGLERDRLAMLLAVLHRHAGVATGDQDVFVNAVGGVRISE
                     PAADLAVLLAIASSLRGKPLPKGFIAFGEVGLAGEVRPAPRGQERLKEAAKLGFSVAL
                     IPRANAPRKADPAFEGVTLHTVERVDEAMALLRTLE"
BASE COUNT          330 a          820 c          788 g          364 t
ORIGIN      
        1 cgtggtggcg cgtgtagctg ctggtcttga cgcggatgcc gcgcttgagg ccttcttcgc
       61 caaggtaggc gccccaggcc caggcggcca ccatcacgtg gatgcggttg cccttggggc
      121 tgacgcccag cttttcagag ccgatccaca ccagcgggcg caggtagcag ctcttgagct
      181 ggttggcgcg caccacttcc ttctgcgctt cgttgacctg ctcgggcgtg aacgggatgg
      241 ccatgcgcag gatcttggcg ctgttgaaca ggcgctgggt gtgctcctgc aggcggaaga
      301 cggcggtgcc gcccacggtg tcgtaggcgc gcacgccctc gaaggcgccg cagccgtagt
      361 gcagggtgtg cgtcagcacg tggatcttgg catcgcgcca gtcgaccagc tggccgtcca
      421 gccagatctt gccgtcgcgg tcggaaagcg ggggggtgct catggtgggg gtcctcgggg
      481 tctgcggaac gggcgcgggt tgcgggcggg tgcgcgcaaa ccgtgaattc tattgcttcg
      541 aatcttgaac ttcgaaccgt tcaccctgag cctgtcggag ggcttcgaca ggctcagccc
      601 gaacggcatt ccagtcagcc aaaggccgaa tcaatgggcc tcgggcgtgc tggcctggac
      661 gtccggatcg gccgggcgtt cgagccgcca gcgcaccaga cggccagctt cgaactcggt
      721 caccacatgc gactggcccg ggtcgctcca gcgatacacc tcgggctgcg cctcgggcgc
      781 cgacagtcgc tcgcccagcg actgggtcag cgccatgaca tgcagcagcg gcaggccctg
      841 acgcagcttg gcgtggagca tcacggcgct gccgatgtgg ccgatcggcc gctcgttggc
      901 gcgcttcatc accgtcagca ggcggtggta gtgcagcagg aaccacagca ccagcccgct
      961 gcccagcagc gccagcccgg cccagccacc ggcgcgccag gcgccggccc ccagcgccac
     1021 ggcgcccagc atcacccaca gacgttgcca cttcatggcg ctattgtccc ccgccaccac
     1081 gaccccacga aacgctatcg ttaatgtagc tgacggcgct tactgcataa gcgctggagg
     1141 tcaatttcat tcccaaccgt cggtggctgc ggcggccatc atcgcggcgt cactccaggg
     1201 tgcgcagcag ggccatggcc tcgtccacgc gctcgacggt gtgcagcgtc acgccttcga
     1261 acgccgggtc ggcctttctg ggcgcgttgg cgcgcgggat cagcgccacc gaaaaaccca
     1321 gcttggcggc ttccttcagt cgctcctgac cgcgcggcgc cgggcgcacc tcaccggcca
     1381 ggccgacctc gccaaaggcg atgaagccct tgggcagcgg cttgccgcgc agcgacgagg
     1441 caatcgccag cagcaccgcc aggtcggccg ccggctcgct gatgcgcacg cccccgacgg
     1501 cgttgacgaa cacgtcctgg tcgcccgtgg cgacgccggc gtggcggtgc agcaccgcca
     1561 gcagcatggc caggcggtcg cgctccagcc ccaccgacag ccgccgcggg ctgggtccgc
     1621 cgctgtcgac cagcgcctgc acttccacca gcagcgggcg cgtgccctcc agcgtcacca
     1681 gcacgcagct gcccggcacc ggttcggcgt gctgggccag gaagatggcg ctggggttgc
     1741 tcaccccttt caggccgcgc tcggtcatgg cgaacacgcc gatttcgttg acggcgccaa
     1801 agcggttctt gatggcgcgg atcaggcggt agctgctgtg cgtgtcgccc tcgaagtaca
     1861 gcacggtgtc gaccatgtgc tccagcacgc gcgggccggc cagctgaccg tccttggtca
     1921 catggcccac cagcaccacg gcgcagccgc tggccttggc ccagcgcgtc aggtgggcgg
     1981 cgcattcgcg cacctgggcc acgctgcccg gggcgctggt gagctggtcg ctgtagacgg
     2041 tctggatcga gtcgatcacc gccaccgcgg gctgctgtgc actcagggtg gcgaggattt
     2101 tttccagctg gatctcggcc agcaccgcca cctgcgagtg ggcaatgccc agacgccgcg
     2161 cgcgcagcgc cacctgggcg ccgctttctt cgcccgtgac gtacagcatg cccccgcgct
     2221 gcgccgcggc gccggtggcg ctgccccccg agggggcgct cgcaccttgg ggcggcccgg
     2281 cggtgctcgg gtcacggccc cc
//
LOCUS       DAOOOG010000298         2296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_58316_length_2296_cov_38.548416, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000298 DAOOOG010000000
VERSION     DAOOOG010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58316_length_2296_cov_38.548416"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1250)
                     /locus_tag="PK242_13615"
     CDS             complement(114..1250)
                     /locus_tag="PK242_13615"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOZ95104.1"
                     /translation="MSTTQAPVLFDTLATASGHRFGRATLNTPASLNALSLAMVDLLD
                     PQLAAWAADPQIAGVLLDAAGDKAFCAGGDVVALAREILALHSQQPGAVPQAAADFFE
                     REYRLDHRIHRYPKPLLVWGHGIVMGGGIGLMAGASHRVATPKTRLAMPEIGIGLYPD
                     VGGSWLLGRLPGKAGPFLALTGASINAADALFAGMADFALPHEAHGALLQAIGQARWQ
                     GDQAADGAQLSHLLQRLGEGTALPASPLRTHLDRINAVIGHDRLSDIAPRLAALAQDA
                     DPWLAQAGASFVKGSPTSAALGLELQRRCRHLSLADTFRLEWQASVGCCAHQDFAEGV
                     RALLIDKDKAPRWQPATLEQVTPQWIEDHLRPRFAGAHPLADLA"
     gene            complement(1268..>2296)
                     /locus_tag="PK242_13620"
     CDS             complement(1268..>2296)
                     /locus_tag="PK242_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ95105.1"
                     /translation="GELGFCGLYAPESAGGLALPRLDATLVFEQMAAVDPSTTAFITI
                     HNMATWMLGTWATPAVREHWGPLLTSGQKLASYCLTEPGAGSDAASLKTRAERVGGEY
                     VIHGGKAFISGAGSTDVLVLMARTGGGGAGGISAFVVPADLPGIHYGKKEEKMGWNSQ
                     PTRQIAFDGVRVPADHLLGQEGEGFRIAMKGLDGGRINIATCSVGAAQGALQHAQRYL
                     HERQQFGQPLASFQALQFKLADMATELVAARQMVRLAASKLDRGAPDASTYCAMAKRF
                     ATDAGFAVINDALQLHGGYGYIREYPLERLLRDARVHQILEGTNEIMRVIIARRLLQD
                     SPMDGLTG"
BASE COUNT          295 a          770 c          893 g          338 t
ORIGIN      
        1 tggccatcgg gccgcccatg tggcccaggc cgatgaaggc gatgcgggtg ggggtgctca
       61 tgtgctgtct ccttgaagca atggatgggg tgtgtacggg tgcgggggct cgctcaagcc
      121 aggtcggcca gcgggtgcgc gccggcaaag cgcggccgca ggtggtcttc gatccactgc
      181 ggcgtgacct gctccagcgt ggccggctgc cagcgcggcg ccttgtcctt gtcgatcagc
      241 agggcgcgca cgccttcggc gaagtcctgg tgcgcgcagc agcccaccga ggcctgccat
      301 tcgagccgga aggtgtcggc cagcgacagg tggcggcagc gccgctgcaa ttccagcccc
      361 agggcggccg aggtgggcga gcccttgacg aagctggcgc cggcctgcgc cagccagggg
      421 tcggcgtcct gcgccagcgc cgccaggcgc ggggcgatgt cgctcaggcg gtcgtggccg
      481 atgacggcgt tgatgcggtc caggtgggtg cgcaggggcg atgcgggcag cgccgtgcct
      541 tcacccagcc gctgcagcag gtggctgagc tgcgcgccgt cggccgcctg atcgccctgc
      601 cagcgcgcct ggccgatggc ctgcagcagc gcgccatgcg cctcgtgcgg cagcgcgaag
      661 tcggccatgc cggcaaacag cgcgtcggcg gcgttgatgg aggcgccggt cagcgccagg
      721 aaaggccccg ccttgcccgg cagccgcccc agcagccagg agccgccgac gtcggggtac
      781 aggccgatgc cgatctcggg catggccagg cgtgtcttgg gcgtggccac gcggtgcgag
      841 gcgccggcca tcagtccgat gccgccgccc atgacgatgc cgtgccccca caccagcagc
      901 ggcttggggt agcggtggat gcggtggtcg agccggtatt cgcgctcgaa gaagtcggcc
      961 gccgcctgtg gcacggcgcc gggctgctgc gagtgcaggg caaggatctc gcgcgccagc
     1021 gccaccacgt cgccgccggc gcagaaggcc ttgtcgccgg cggcgtcgag cagcacgccg
     1081 gcgatctgcg ggtcggcggc ccaggcggcc agttgcgggt cgagcagatc gaccatggcc
     1141 agcgacagcg cgttcaggct ggccggggtg ttgagcgtgg cgcggccgaa gcggtggccg
     1201 ctggcggtgg cgagggtgtc gaacaggacg ggggcttggg tggtgctcat ggcgtcggtc
     1261 gtgggggtca gccggtcagc ccgtccatgg ggctgtcctg cagcaggcgg cgcgcgatga
     1321 tgacgcgcat gatctcgttg gtgccttcca ggatctggtg cacgcgggcg tcgcgcagca
     1381 ggcgctccag cgggtattcg cggatgtagc cgtagccgcc gtgcagctgc agcgcgtcgt
     1441 tgatgacggc aaaaccggcg tcggtggcaa agcgcttggc catggcgcag taggtgctgg
     1501 cgtcgggcgc gccgcggtcc agcttgctgg cggccaggcg caccatctgg cgcgccgcca
     1561 ccagctcggt ggccatgtcg gccagcttga actgcagcgc ctggaagctg gccagcggct
     1621 ggccgaactg ctggcgctcg tgcaggtagc gctgggcgtg ctgcagcgcg ccctgggcgg
     1681 cgccgaccga gcaggtggcg atgttgatgc gcccgccgtc cagccccttc atggcgatgc
     1741 gaaagccttc gccctcctgg cccagcaggt ggtcggccgg cacgcgcacg ccgtcgaagg
     1801 cgatctggcg cgtgggctgg ctgttccagc ccattttttc ttccttcttg ccgtagtgga
     1861 tgccgggcag atcggccggc accacgaagg cgctgatgcc accggcgccg ccgccgccgg
     1921 tgcgcgccat cagcaccagc acgtcggtgc tgccggcgcc gctgatgaag gccttgccgc
     1981 cgtggatcac gtactcgccg cccacccgct cggcgcgcgt cttgagcgag gcggcgtcgg
     2041 agccggcgcc cggctcggtc aggcagtagc tggccagctt ctggccgctg gtcagcagtg
     2101 gcccccagtg ctcgcgcacc gcgggcgtgg cccaggtgcc cagcatccag gtggccatgt
     2161 tgtggatggt gatgaaggcg gtggtggacg gatcgaccgc cgccatctgc tcgaacacca
     2221 gggtggcgtc cagccgcggc agggccaggc cgccggcgga ctcgggcgcg tacaggccgc
     2281 aaaagcccag ctcgcc
//
LOCUS       DAOOOG010000299         2288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_58604_length_2288_cov_28.198388, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000299 DAOOOG010000000
VERSION     DAOOOG010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58604_length_2288_cov_28.198388"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PK242_13625"
     CDS             complement(<1..365)
                     /locus_tag="PK242_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOZ95106.1"
                     /translation="MLSIIQAAGWPIWPLILCSILALAFAIERFVQLQSRRVLGAQLV
                     DEAIGVSRESVPPPDMVRQLELHSALGEVLAAGWRAINANPRCSPEEMRAAMEAAGRQ
                     VAHRLERYLPALATIASAAP"
     gene            741..2150
                     /gene="xseA"
                     /locus_tag="PK242_13630"
     CDS             741..2150
                     /gene="xseA"
                     /locus_tag="PK242_13630"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOZ95107.1"
                     /translation="MNTGFEPAAPALAPRVWAVGALARAVADLLQARFNPVAVRGEVS
                     GFTRASSGHCYFALKDDTGQLRCAMFRRAAGLLDFVPREGDAVELRGRLTVYEPRGDL
                     QLVVETMQRAGQGALFEQFLRLKARLQAEGLFDPARKRPLPALPRGIGLVTSRGAAAL
                     HDVLSTLARRVPHIPVLLVPAAVQGAGAPAELSQALQKLYLLAQSGQAPQANLTRNAG
                     APPIDLILLVRGGGSIEDLWAFNDEPLARTIAASPVPVVSGVGHETDFTIADFVADLR
                     APTPTAAAELVGAPQAQWLAELQARAERLQRGLRHRLDAEAQRLDWLNAHLARPSQAV
                     AAERVRLAQLQQRLQHAQALRLERERARWQTLAARLPRSLTAPQAAEGARLQRLAERL
                     PAALARQLGSADERLQRAALRLSLLDPRLVLERGYAWLEDAQGRPVIRAAQTHDGQAL
                     AATLADGRVDLTVRGRRSG"
BASE COUNT          314 a          815 c          847 g          312 t
ORIGIN      
        1 ggcgccgccg aggcgatggt ggccagcgcc ggcaggtagc gctccagccg gtgcgccacc
       61 tggcggccgg cggcttccat ggcggcgcgc atttcttccg ggctgcaacg gggattggcg
      121 ttgatggcgc gccagccggc cgccagcacc tcgcccaggg cgctgtgcag ctccagctgg
      181 cgcaccatgt cgggcggcgg cacgctttcg cgcgagacgc cgatggcttc gtccaccagc
      241 tgggcaccca gcacgcggcg gctctgcaac tggacgaagc gctcgatggc gaaggccagc
      301 gccaggatcg aacacaggat gagcggccag atcggccagc cggcggcttg tatgatcgac
      361 agcaaggggt gctttctcgg gcgcaggcgc ggcccgcaat gtcggaaaaa agagtgcagg
      421 cgattatggc gcagcccgtg tgccccaagc gccagccgcg cgaccaggcc ccggcagcgt
      481 ggtgaaccga cggcacgtcc gggtaactac cgatgttgtg gataactttg tggaatattc
      541 gcccggtctg gcccgttcca gctgcggccg ccacgattgt gaccgattgt gcgtcgcagc
      601 atcaagaaaa ttgtgtttga aatcaatggc ttgcaccgat ctctcgtggt tttcggcggg
      661 tggcccgagg ctgatggggg cgggcgccgc gctgtggagt actggcgcgg cctgggcccc
      721 ggaatcggcc gccagagcgc atgaatactg ggtttgaacc ggcagcgcca gcattggcgc
      781 cgcgcgtctg ggcggttggg gcgctggcgc gagccgtggc ggacctgttg caggcgcgct
      841 tcaacccggt ggcggtgcgc ggcgaggtgt cgggcttcac gcgcgcttcc agcgggcact
      901 gttatttcgc gctcaaggac gacaccggcc agctgcgctg cgccatgttc cgccgcgccg
      961 ccgggctgct ggacttcgtg ccgcgcgagg gtgacgcggt cgagctgcgc gggcgcctga
     1021 cggtgtacga gccgcgcggc gacctgcaac tggtggtgga aaccatgcag cgcgccggtc
     1081 agggcgcgct gttcgagcag ttcctgcggc tgaaggcgcg gctgcaggcc gagggtctgt
     1141 tcgaccccgc ccgcaagcgc ccgctgccgg cgctgccgcg cggcatcggc ctggtgacgt
     1201 cgcgcggcgc cgccgccctg cacgacgtgc tcagcaccct ggcgcgccgc gtgccgcaca
     1261 tcccggtgtt gctggtgccg gcggcggtgc agggcgccgg ggcgccggcc gagctgtccc
     1321 aggcgctaca aaaactgtat ctgctggcgc agtctggaca agcgccgcag gccaatctga
     1381 cacgcaacgc gggggcgccg ccgatcgacc tcatcctgct ggtgcgcggc ggcggctcga
     1441 tcgaggacct gtgggcgttc aacgacgagc cgctggcgcg caccatcgcc gccagcccgg
     1501 tgccggtggt cagcggcgtc ggccatgaaa ccgacttcac catcgccgac ttcgtggccg
     1561 acctgcgggc gcccacgccc acggcggcgg ccgaactggt gggcgcgccg caggcgcagt
     1621 ggctggccga gctgcaggcg cgggccgaac gcctgcagcg cgggctgcgt caccggctcg
     1681 acgccgaggc gcagcgcctg gactggctga acgcccacct ggcgcgcccg tcgcaggcgg
     1741 tggcggccga gcgggtgcga ctggcgcaac tgcagcagcg gctgcagcac gcccaggcgc
     1801 tgcgcctgga gcgcgagcgg gcccgctggc agacgctggc ggcgcgcctg ccgcgcagcc
     1861 tgacggcacc gcaggcggcc gagggcgcgc gcctgcagcg cctggccgaa cgcctgcccg
     1921 ccgcgctggc gcgccaactg ggcagcgccg acgagcgcct gcagcgcgcc gccctgcggc
     1981 tgtcgctgct cgatccacgg ctggtgctgg agcgcggcta cgcctggctg gaggacgccc
     2041 agggccggcc cgtcatccgc gccgcccaga cgcacgacgg ccaggcgctg gccgccaccc
     2101 tggcggacgg cagggtcgat ctgacggtgc gcgggcgccg atcgggctga cttcctacaa
     2161 tcgaccgttg ccgcgcgccg gcccctggcc caggcgccca tccattgcca cccacgaact
     2221 caaggagaac ccacatggaa cacaccctgc cgccgctgcc ctacgccatc gacgccctgg
     2281 ccccgcac
//
LOCUS       DAOOOG010000300         2274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_59112_length_2274_cov_18.126183, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000300 DAOOOG010000000
VERSION     DAOOOG010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59112_length_2274_cov_18.126183"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PK242_13635"
     CDS             <1..744
                     /locus_tag="PK242_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOZ95108.1"
                     /translation="VGGADAIDSIEAELQNLMLRIRYAQVPVVAAIHGMALGGGCELA
                     VHCARRVAHMESYIGLVEVGVGLVPGAGGLTYIARRAAEAATGSTGKDLLPFLTEGFT
                     AAAMAKVGTSAIESRKLGYLRDSDIIVPHRDELLFVAINEARAMAAGGWRAPLPRPFP
                     VAGRSGKATIMGSLVNMQGGGFISEFDQHIATLIANVVCGGDVDAGTLVDEAYLMKLE
                     RQAFCQLIAHPKTHERILGMLNTGKPVRN"
     gene            756..1298
                     /locus_tag="PK242_13640"
     CDS             756..1298
                     /locus_tag="PK242_13640"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HOZ95109.1"
                     /translation="MVHNHPHPSPLPPAGEGEKPAHADAFPTVGEGEKPARAGSLSRV
                     RERAGVRVQNARALRASGTDAEQALWRHLRSRQLNGHKFRRQHPIGPYIADFACVEAR
                     LVVELDGGQHAQADALKHDERRTQFLLAQGWHVLRFWNHDMLAEPAGVLQTIVQALAH
                     PHPHPLPPAGEGVEQPWARA"
     gene            1336..>2274
                     /locus_tag="PK242_13645"
     CDS             1336..>2274
                     /locus_tag="PK242_13645"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOZ95110.1"
                     /translation="MKQVQDAYIVAATRTPIGRSHKGYFRNYRPDDLLATALKAVMAQ
                     APGLDPAAVEDVIAGCAIPEAQQGLNVARIGAVLAGLPNSVGGITVNRFCASGLSAVA
                     MAADRIRVGEAEVMIAAGVESMSMVPMMGHSPSLSPSIFAPDSDVGIAYGMGLTAEKV
                     AQQWKVSREAQDAFAVESHRRAIAAQQAGEFADEITPVEVTDRGFDLASGQTTSRTRT
                     VNLDEGARPDTTLEGLAKLRTVFAARGSVTAGNSSQTSDGAGALILASEAAVKRFGLT
                     PLARFVSYASRGVPPSIMGIGPIEAIPAALRYAGLKQ"
BASE COUNT          379 a          807 c          771 g          317 t
ORIGIN      
        1 gtcggcggcg ccgacgccat cgacagcata gaggccgagc tgcagaacct gatgctgcgc
       61 atacgctatg cgcaggtgcc ggtggtggcg gccatccatg gcatggcgct cggcggcggc
      121 tgcgagctgg cggtgcactg cgcgcgccgc gtggcccaca tggaaagcta catcggcctg
      181 gtcgaagtgg gcgtgggcct ggtgcccggt gccggcggcc tgacctacat cgcccgccgc
      241 gcggccgaag ccgccaccgg gagcaccggc aaggacctgc tgcccttcct gaccgaaggc
      301 ttcaccgccg ccgccatggc caaggtcggc accagcgcca tcgaatcgcg caagctcggc
      361 tatctgcgcg actccgacat catcgtgccg cacagggacg aactcctgtt cgtcgccatc
      421 aacgaggccc gcgccatggc cgccggtggc tggcgcgcgc cgctgccgcg ccccttcccc
      481 gtggccggca ggagtggcaa ggcgaccatc atgggttcgc tcgtcaacat gcagggcggc
      541 ggcttcatca gcgagttcga ccagcatatc gccaccctga tcgccaacgt ggtctgcggc
      601 ggcgacgtgg acgcaggcac cctggtcgat gaggcctacc tgatgaagct cgagcgccag
      661 gcgttctgcc agctcatcgc ccatcccaag acgcatgagc gcatcctggg catgctcaat
      721 acgggcaagc cggtgaggaa ctgaggctgc aggccatggt gcacaaccac cctcacccca
      781 gccctctccc gccagcggga gagggtgaaa aaccggcgca tgccgacgcc ttcccgacag
      841 tgggagaggg cgaaaaaccg gcgcgcgctg gctccctctc ccgcgtgcgg gagagggcag
      901 gggtgagggt gcaaaacgcg cgcgcactgc gtgccagtgg cacggatgcc gagcaagcgc
      961 tgtggcgcca cctgcgcagc cgacaactga atgggcacaa gttccgtcgc cagcacccca
     1021 tcggccccta catcgcagac tttgcctgcg tagaggccag gctggtggtg gagctcgatg
     1081 gcgggcagca tgcccaggcc gatgcgctca agcatgacga gcggcgcacg caatttctgc
     1141 tggcccaggg ctggcatgtg ctgcgctttt ggaaccacga catgctggca gagcccgccg
     1201 gggtgctgca aaccattgtg caggcgttgg cccaccctca cccccaccct ctcccgccag
     1261 cgggagaggg agttgaacag ccatgggcgc gggcctgagc ctgccgcctt cgagaaaccg
     1321 caaggagcca tctccatgaa acaagtacaa gacgcctaca tcgtcgccgc cacgcgcacg
     1381 cccatcgggc gctcgcacaa gggctatttc cgcaactacc ggcccgacga cctgctggcc
     1441 accgcgctca aggccgtgat ggcgcaggca cccgggctgg acccggccgc ggtcgaggat
     1501 gtgattgccg gctgcgccat ccccgaggcc cagcagggcc tgaacgtggc gcgcatcggc
     1561 gccgtgctgg ccggactgcc caacagcgtg ggcggcatca cggtgaaccg cttctgcgcc
     1621 tcgggtcttt cggccgtggc catggcggcc gaccgcatcc gcgtgggcga ggccgaggtg
     1681 atgatcgccg ccggcgtgga gagcatgagc atggtgccca tgatgggcca cagccccagc
     1741 ctgtcgccca gcatcttcgc gcccgacagc gacgtcggca ttgcctacgg catgggcctg
     1801 acggccgaga aggtggcgca gcagtggaag gtctcgcgcg aggcgcagga cgcctttgcc
     1861 gtggagtcgc accgccgcgc cattgctgcg cagcaggccg gcgagtttgc cgacgagatc
     1921 acccccgtgg aggtgaccga ccgcggtttt gacctggcca gcggccagac caccagccgc
     1981 acgcgcaccg tcaacctcga tgagggtgcc cgccccgaca ccacgctcga gggcctggcc
     2041 aagctgcgca cggtgtttgc cgcgcgcggc tcggtgacgg cgggcaacag cagccagacc
     2101 agcgacggcg cgggcgccct catcctggcc agcgaggccg cggtcaagcg ctttggcctg
     2161 acgccgctgg cgcgctttgt cagctacgcc agccgcggcg tgccgccgtc catcatgggc
     2221 atcggcccca tagaggccat tcccgccgcg ctgcgctacg ccggcctgaa gcag
//
LOCUS       DAOOOG010000301         2254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_59918_length_2254_cov_26.466576, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000301 DAOOOG010000000
VERSION     DAOOOG010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59918_length_2254_cov_26.466576"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PK242_13650"
     CDS             complement(<1..259)
                     /locus_tag="PK242_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOZ95111.1"
                     /translation="MTKAKPAHRRILLKLSGEALMGDDAFGINRATIERIVGEIAEVT
                     RMGVQVAVVIGGGNIFRGVAGGSVGMDRATADYMGMLATVMN"
     gene            complement(386..1279)
                     /gene="tsf"
                     /locus_tag="PK242_13655"
     CDS             complement(386..1279)
                     /gene="tsf"
                     /locus_tag="PK242_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOZ95112.1"
                     /translation="MAAITASMVAELRAKTDAPMMECKKALTEADGDMAKAEELLRVK
                     LGTKAGKAASRVTAEGVIAAHIAGTTGAMIEVNCETDFVTKNDMFIAMAQAAARLVAE
                     HNPADVTALGALAYEQDGYGPTLEDVRKGLIGKIGENMSFRRFVRKAGGAQLTSYLHG
                     TRIGVLVEFQGDEVAARDVAMHVAAMKPKALTAAEVPADLVETERSVAAAKAAESGKP
                     ADIAAKMVEGSVQKFLKEVSLFNQPFVKNDKQSVEQMLKEKATQVSGFTLYVVGEGIE
                     KKQDDFAAEVAAQVAAAQGKA"
     gene            complement(1409..2164)
                     /gene="rpsB"
                     /locus_tag="PK242_13660"
     CDS             complement(1409..2164)
                     /gene="rpsB"
                     /locus_tag="PK242_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOZ95113.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRFWNPKMAPFIFGHRNKIHIINLEK
                     SLPMLQEAQKFARQLAANRGTILFVGTKRQAREIVAEEARRCGMPYVEQRWLGGMLTN
                     FKTVKTSIKRLKDMQAQQEAGLDSMSKKEQLMFTREMEKLERDIGGIQDMGALPDAIF
                     VIDVGYHKIAVSEAKKLGVPLIGVVDSNHNPDGIDYVIPGNDDSARAVTLYARALADA
                     VLEGKANAVNDVVKAVQAEGEDEFVEVEDDGAA"
BASE COUNT          319 a          733 c          758 g          444 t
ORIGIN      
        1 cgttcatcac cgtggccagc atgcccatgt agtcggcggt ggcgcggtcc atgcccaccg
       61 agccgccggc gacgccgcga aagatgttgc cgccgccgat caccaccgcc acctgcacgc
      121 ccatgcgcgt cacctcggcg atctcgccga cgatgcgctc gatggtggcg cggttgatgc
      181 cgaacgcatc gtcgcccatc agggcttcgc cggagagctt gagcaggatg cgacgatggg
      241 caggcttggc tttggtcatg ggcggggttt gggggcggcg gctggcggtc gagtttagcg
      301 gctgatcggc ggcggcgtcc ggtgcggcgg gtacacaaaa aaggcgcgcc gcctgcgcga
      361 cgcgcccgtg gcatggcgcg gccgatcagg ccttgccctg cgcggcggcc acctgcgcgg
      421 ccacctcggc ggcgaagtcg tcctgcttct tctcgatgcc ctcgcccacc acgtacagcg
      481 tgaagccgct gacctgggtg gctttttcct tcagcatctg ctcgacgctc tgcttgtcgt
      541 tcttcacgaa cggctggttg aacagcgaga cttccttgag gaatttctgc accgaacctt
      601 cgaccatctt ggcggcgatg tcggccggct tgccggactc ggcggccttg gcagcggcca
      661 cgctgcgctc ggtttcgacc agatcggccg gcacctcggc ggcggtgagc gccttgggct
      721 tcatggcggc cacgtgcatg gcgacgtcgc gcgcggccac ttcatcgccc tggaactcca
      781 ccagcacgcc aatgcgcgtg ccgtgcaggt agctggtcag ctgggcgccg ccggccttgc
      841 gcacgaagcg gcggaagctc atgttctcgc cgatcttgcc gatcaggccc ttgcgcacgt
      901 cttccagcgt ggggccatag ccgtcctgct cgtaggccag tgcgcccagg gcggtcacgt
      961 cggccgggtt gtgctcggcc accagcctgg cagcggcctg ggccatggcg atgaacatgt
     1021 cgttcttggt gacgaagtcg gtttcgcagt tgacctcgat catggcgccg gtggtgccgg
     1081 caatgtgcgc ggcgatcacg ccttcggccg tgacgcggct ggcggccttg ccggccttgg
     1141 tgcccagctt gacgcgcagc agctcctcgg ccttggccat gtcgccatcg gcctcggtca
     1201 gggccttctt gcactccatc atgggggcgt cggtcttggc gcgcagttcg gcgaccatgc
     1261 ttgcggtaat tgctgccatg tgtgattact ccagatgtcg gtggggcggc ccgcacgggc
     1321 gggcgccacc ccgggaaacg aaacattgaa aaaggggcct gtgcgcccct ttgccagcgg
     1381 tcgccaggcg ggcgacgcag gtggacgatc aggcggcgcc gtcgtcctcg acttcgacga
     1441 actcgtcctc gccttcggcc tgcacggcct tgaccacgtc attgacggcg ttggccttgc
     1501 cttccagcac ggcgtcggcc agcgcacggg cgtacagcgt gacggcacgc gcggagtcgt
     1561 cgttgcccgg aatcacgtag tcgataccgt ccgggttgtg gttggagtcg accacgccga
     1621 tcagcggcac gcccagcttc ttggcttccg agacggcgat tttgtggtag ccgacgtcga
     1681 tcacgaagat ggcgtccggc agcgcgccca tgtcctggat gccgccgatg tcgcgttcga
     1741 gcttttccat ctcgcgcgtg aacatcagct gctccttctt gctcatgctg tccagaccgg
     1801 cttcctgctg ggcctgcatg tccttgaggc gcttgatgga ggtcttgacg gtcttgaagt
     1861 tggtcagcat gccgcccagc cagcgctgct cgacgtaggg catgccgcag cggcgggctt
     1921 cctcggcgac gatctcacgc gcctggcgct tggtgccgac gaacaggatg gtgccgcggt
     1981 tggccgccag ctggcgcgcg aatttctgcg cttcctggag catcggcagc gatttttcca
     2041 ggttgatgat gtggatcttg ttgcgatggc cgaagatgaa aggggccatc ttggggttcc
     2101 agaagcgcgt ctggtggcca aagtgcacac cggcttccag catctgacgc atggtcacgg
     2161 acatggaaat taccttctca aggttgggtc tgaaatccag ccccctcaat caccggccgg
     2221 gccgttgaaa cggccggcag cgacacctcg tcgg
//
LOCUS       DAOOOG010000302         2246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_60233_length_2246_cov_21.863076, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000302 DAOOOG010000000
VERSION     DAOOOG010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60233_length_2246_cov_21.863076"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PK242_13665"
     CDS             complement(<1..507)
                     /locus_tag="PK242_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOZ95114.1"
                     /translation="MPVLPDAPTAADLPLLAGLNPEQKAAVTLPPEPALILAGAGSGK
                     TRVLTTRIAWLLQTGQVSPGGVLAVTFTNKAAKEMLTRLSAMLPLNVRGMWIGTFHGL
                     ANRFLRAHHQLAGLPQAFQILDTQDQLSAIKRLVKQFNIDEERYPPRQLQWFIAGCKE
                     DGLRPRDVE"
     gene            638..1043
                     /locus_tag="PK242_13670"
                     /pseudo
     CDS             638..1043
                     /locus_tag="PK242_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518706.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
     gene            1231..1851
                     /locus_tag="PK242_13675"
     CDS             1231..1851
                     /locus_tag="PK242_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95115.1"
                     /translation="MAGFFLAGTQRPASVAARAAIASDRPHHRNGALTMTQPPERFIL
                     DGETWSMNQRPHFPRAHRHIRRTPPPADLSSEAGRQWTWHQSPDCPRGYVGTWTVDKG
                     RLYLVHLSGVWQVRPGQRVFARWFSGELLVFKGAPLPHVRRMHDLIHEHEVVIQVMRG
                     RVQAMFANDDIGKKRGEVYLTPSERRHRQRQTERLRRFVDQLDEPQ"
     gene            complement(2129..>2246)
                     /locus_tag="PK242_13680"
     CDS             complement(2129..>2246)
                     /locus_tag="PK242_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HOZ95116.1"
                     /translation="KDLERGKWVKVLGWEGPEAAHKEVTDGLASYRKAHPQR"
BASE COUNT          389 a          758 c          739 g          360 t
ORIGIN      
        1 ttccacgtcg cgcgggcgca ggccgtcttc cttgcagcca gcgatgaacc actgcagctg
       61 gcgcggcggg tagcgctctt cgtcgatgtt gaactgcttg accaggcgtt tgatggccga
      121 cagctggtcc tgcgtatcca gaatctgaaa cgcctgcggc aaacccgcca gctggtggtg
      181 cgcgcgcaga aagcggttgg caaggccgtg aaaggtgccg atccacatgc cgcgcacatt
      241 gagcggcagc atggccgaca ggcgcgtgag catctccttg gccgctttgt tggtgaaggt
      301 cacggccagc acgccgccgg gcgagacctg cccggtctgc agcagccagg cgatgcgcgt
      361 ggtcagcacc cgcgtcttgc ccgaaccggc gccggccagg atcagcgcgg gctcgggcgg
      421 cagggtgacg gcggctttct gttcggggtt caggccggcc agcaggggca gatcggcggc
      481 cgtgggcgca tcagggagta caggcatgca tcgattgtag gaaccgtccc caaggcgccg
      541 ccgcatcgct gcagcaacac agcgtgcatg ccgagcacgt cggccacgtt caagtggtcg
      601 cacggcacgg cttgccggaa gtcgcagagc gtggtcgatg gaagttgtgg tggcacatgt
      661 aacaggtcaa ctcgcgggtg aggacggcgg gcgcgagttg gtggtgtgcc agtgggggct
      721 gattccgtgg tttgcaccgc aggcgcgcct gccgcacagc acccacaacc cacacagcga
      781 cgagttgccg agcaaggcaa ccctggacgc ggggccagcg ctgcatcatg ccggcgcagc
      841 cgttcgacaa gccgtgcggg gagacggagc gcatcgtgtg gtggcgcttt cgccgcgccg
      901 acggccaacc ctggggtctg gcgggactgt ggaacacctg gatcgatcgc gccagggccg
      961 aggcgcacga gagctacacc atgctgacga tcaacgccga ccagcacccc ctgatgcgcc
     1021 acatggacaa gcccgatccg aagttcgcgt acggctgagc gcctgcgctc catcgtccga
     1081 tgctggagcg gcgacgcaac actccgcgcg cctgcatggc ggtgccgatc gaccatggaa
     1141 ctttcaagcc cgatgtcgat ctaacactaa cacagcaagc gcgagcagcc atcttttcga
     1201 tagccacttt gcttggcgcc cggcccgccc atggcgggtt tctttttggc gggtacgcaa
     1261 cggcccgcat cggtcgccgc acgcgcggcc attgcatccg atcgacctca ccaccgcaat
     1321 ggagccttga ccatgacgca gccacccgag cggttcattc ttgatggcga aacctggtcg
     1381 atgaaccagc ggccacattt tccccgcgca caccggcaca ttcggcgcac gccccctcca
     1441 gccgaccttt ccagcgaagc tggccggcag tggacgtggc accagagccc ggattgcccg
     1501 cgcgggtacg tcggcacctg gacggtcgac aagggccgcc tctacctggt gcacctgtca
     1561 ggcgtctggc aggtacgccc gggccagcgg gtgtttgcgc gctggttcag cggcgagctg
     1621 ctggtgttca agggagcgcc gctgccgcat gtgcgcagaa tgcatgacct gattcacgaa
     1681 cacgaagtgg tgatccaggt catgcgcggt cgggtgcagg cgatgttcgc caatgacgac
     1741 atcggcaaga agcgtggcga ggtgtacctc acaccatccg agcgccgaca ccgccagcgc
     1801 cagaccgaga ggttgcgccg ctttgtggat cagctcgacg aaccccagtg acggcacctg
     1861 cgcgccgagc cgccacccca tgccccgcgc cagccgcagc ggcgcagaac gcacggtgtt
     1921 gcgccgtccg gccgcgcccc gatcgctccc gacaagggca cactcgccct gcctgcaggc
     1981 ccccttgcac ggggttgccg attcacccca cgcaggggcg ctggtgtcgg cctgtccgca
     2041 cgcacgaccc gcaacaagct accaaaaaca tagccatccg cgcttgcctg gtgagtgcgg
     2101 atagctcatt cgttaccgaa ataccggctc agcgctgggg atgcgccttg cggtagctgg
     2161 cgaggccgtc ggtgacttcc ttgtgcgccg cctcggggcc ttcccagccc agcaccttga
     2221 cccacttgcc gcgctccaga tccttg
//
LOCUS       DAOOOG010000303         2234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_60677_length_2234_cov_27.429555, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000303 DAOOOG010000000
VERSION     DAOOOG010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60677_length_2234_cov_27.429555"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..716)
                     /gene="ispF"
                     /locus_tag="PK242_13685"
     CDS             complement(213..716)
                     /gene="ispF"
                     /locus_tag="PK242_13685"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOZ95117.1"
                     /translation="MTDAEPSRLPALRIGEGWDVHALVPGRPLVIGGVTVPHTHGLLG
                     HSDADVLLHAVTDALLGAAGLGDIGRHFPDTDARFAGADSAVLLAEAARRVGAAGWRV
                     GNVDGTVIAQAPKLAPHLPAMVARIAQVLGVGVAQVNVKAKTAERLGPVGQGASIEAR
                     AVVLLFR"
     gene            complement(713..1417)
                     /gene="ispD"
                     /locus_tag="PK242_13690"
     CDS             complement(713..1417)
                     /gene="ispD"
                     /locus_tag="PK242_13690"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOZ95118.1"
                     /translation="MSVQSPRCHALVPCAGSGARAGGVQPKQYQLVAGQPLVRHTLAA
                     LASVARLVRVLVVVAPGDDRLHELPAPLEVAPCGGATRAESVLNGLRHLRATGAADSD
                     WVLVHDAARCLITPAQVDALIDACWQDAVGGLLALPLPDTLKMEDAQGRSAATVERAG
                     KWLAQTPQMFRLGALHDALAAHAARGFAGITDEASAIEAAGLRPLLVRGSAHNVKVTY
                     PEDFALAEAVLRHRTP"
     gene            complement(1612..>2234)
                     /locus_tag="PK242_13695"
     CDS             complement(1612..>2234)
                     /locus_tag="PK242_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOZ95119.1"
                     /translation="EAAPEDLRYQAARFHVVGTDHAIGLFELAGRQPGAHIQVEHTHT
                     VAGPSWPNGAHICEVELDPQTGAVAVVGYTSVNDVGRVVNPLIVRGQLDGGAVQGIGQ
                     ALCEQIVYDAASGQLLTGSLMDYAVPRAHELATCFRTEMDESTPCANNALGVKGVGEL
                     GTIGATPAVVNAVADALARAGRPELAPRLQMPLSPARLWALLQSQG"
BASE COUNT          336 a          806 c          774 g          318 t
ORIGIN      
        1 cgctgcggtc gatttgcagc cggcgatttc caagtccgac agcctcctag gttcaaccta
       61 tggatcggcc ttgggatgcc ttgaataccg ttcgggctga gcttgtcgca gcccgtcgac
      121 aggctcgggg tgaacggttc aaagttcaag attccgaacc cgcaggttca aggacattcc
      181 ggctgcagcg cccgccgggc gggcgccaca agctatcgaa atagaagcac cacggcgcgc
      241 gcctcgatgc tggcgccctg ccccaccggc cccaggcgct cggccgtctt ggccttgacg
      301 ttgacctgag ccacgcccac gcccagcact tgggcgatgc gcgccaccat ggccggcagg
      361 tgcggcgcca gcttcggtgc ctgggcaatc accgtgccat cgacgttgcc cacgcgccag
      421 cccgccgcgc ccacgcggcg cgccgcctcg gccagcagca cggccgaatc ggcgccggca
      481 aaacgcgcgt cggtatcggg aaaatgccgc ccgatgtcgc ccagcccggc cgcgcccagc
      541 agcgcgtcgg tgacggcgtg cagcagcacg tcggcgtcgg aatggcccag cagcccgtgt
      601 gtgtgcggga cggtcacacc accgatcacc agcggccggc caggcaccag ggcgtgtacg
      661 tcccagcctt caccgatgcg cagggcgggc aggcgtgatg gctcggcatc ggtcatggcg
      721 ttcggtggcg cagcacggct tcggccagcg caaagtcttc ggggtaggtc accttgacat
      781 tgtgcgcgct gccgcgcacc agcagcggcc gcaggccggc ggcctcgatc gcgctggcct
      841 cgtcggtgat gccggcaaag ccgcgggccg cgtgcgccgc cagcgcgtcg tgcagcgcgc
      901 ccaggcggaa catctgcggc gtctgcgcca gccacttgcc ggcacgctcg acggtggcgg
      961 ccgagcggcc ctgcgcgtct tccatcttca gggtgtcggg cagcggcagc gccagcaggc
     1021 cgcccacggc atcctgccag caggcgtcaa tcagcgcatc gacctgcgcc ggcgtgatca
     1081 ggcagcgcgc cgcatcgtgc accagcaccc agtcgctgtc ggcagcgcca gttgcgcgca
     1141 gatggcgcag gccgttcagc acgctctcgg cccgcgtggc gccgccgcag ggcgccacct
     1201 ccagcggcgc cggcagttcg tgcagccgat cgtcgcccgg cgccaccacc accagcacgc
     1261 ggaccaagcg cgccacggac gccagcgccg ccagcgtatg gcgcaccagc ggctggccag
     1321 ccacgagctg gtactgcttg ggctgcaccc cacccgcgcg cgcgccgctg ccggcgcagg
     1381 gcaccagggc gtggcagcgg ggggattgaa ccgacatgag gaaattcaac ttggatagct
     1441 ggcggcgctt gatgggtggt cgccagagcc ccgatattgc tacaaatcct gatcgattcc
     1501 gtggggcgct gactgcaagc ttccttgcag aacgaacgcc aaagacacgc ggaagatgcg
     1561 gaaaatcttg aaatcttctg cgcccccggc gtacggatgt ttgccgcggc gctacccctg
     1621 cgactgcagc agcgcccaca gccgcgccgg gctcaatggc atctgcaggc gtggcgccag
     1681 ttcgggccgg ccggcgcgcg ccagcgcatc ggccacggcg ttgaccaccg ccggcgtggc
     1741 gccgatggtg cccagctcgc ccacgccctt gacgcccagc gcgttgttgg cgcagggggt
     1801 ggattcgtcc atctcggtgc gaaagcacgt cgccagctca tgcgcgcgcg gcacggcgta
     1861 gtccatcaga ctgccggtga gcaactggcc gctggcggcg tcgtacacga tctgctcaca
     1921 cagcgcctgg ccgatgccct gcaccgcgcc gccgtcgagc tggccgcgca cgatcagcgg
     1981 gttgacgacg cggccgacgt cgttgacgct ggtgtagccg accacggcca cggcgccggt
     2041 ctgcgggtcg agctcgacct cgcagatgtg cgcgccgttg ggccagctgg gcccggccac
     2101 ggtgtgcgtg tgctcgacct ggatgtgggc gcccggctgg cggccggcca gctcgaacag
     2161 gccgatggcg tggtcggtac cgacgacgtg aaagcgcgcc gcctggtagc gcaggtcctc
     2221 gggcgcggct tcca
//
LOCUS       DAOOOG010000304         2216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_61363_length_2216_cov_26.927811, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000304 DAOOOG010000000
VERSION     DAOOOG010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61363_length_2216_cov_26.927811"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PK242_13700"
     CDS             complement(<1..556)
                     /locus_tag="PK242_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOZ95120.1"
                     /translation="MSQGSDVDAAARLRDWRPRARRLVPGLLVALIVALAATFVSEHY
                     GGPKFLYALLMGMSLHFLSEDARCGPGIEFAARQLVRLGVALLGARIAFGDVLALGGS
                     GVAWLAAAVALTIGFGLAMARALGLPARFGLISGGATGICGISAALAISSALPRDGRT
                     EQQTLMTAIGVAALSTAAMVLYPLV"
     gene            complement(622..>2216)
                     /gene="glmS"
                     /locus_tag="PK242_13705"
     CDS             complement(622..>2216)
                     /gene="glmS"
                     /locus_tag="PK242_13705"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOZ95121.1"
                     /translation="DGAERPGRVTLVHNGIIENHEALRAELRARGYHFSSQTDTETIV
                     HLIDSLYDGDLLAAVQAAVKRLHGSYSIAVLHRDEPRRLVGARQGSPLVLGIGADGKE
                     NFLASDAMALAGVTDQIVYLEEGDLIDVQLGKYWVLDRAGQRVQRPVRTVQAMSGAAE
                     LGPYRHYMQKEIFEQPRAVGDTLEGVQAVVPQLFEAAGQPAAGEDAQATFQAVRQVLI
                     LACGTSYYAGCVAKYWLEALAGIPTQVEIASEYRYRASVPDPDTLVVTISQSGETADT
                     LAALRHAQSLGMRHTLTVCNVATSAMVRECRLAFLTRAGVEIGVASTKAFTTQLVGLF
                     LLALMLAKTRARLGPAQEAEQLDALRRLPGALQAVLALEPQLIDWAEQFTAKEHALFL
                     GRGLHYPIAQEGALKLKEITYIHAEAYAAGELKHGPLALVTSAMPVVTVAPNDALLEK
                     LKSNMQEVRARGGVLYALADADTQIESSEGLYVIRMPGQSGLLSPILHVVPLQLLAYH
                     TAVARGTDVDKPRNLAKSVTVE"
BASE COUNT          313 a          782 c          796 g          325 t
ORIGIN      
        1 ccaccagcgg gtacagcacc atggcggcgg tggacagcgc ggccacgccg atggccgtca
       61 tcagcgtctg ctgctcggtg cggccatcgc gcggcagcgc gctggaaatc gccagcgcgg
      121 ccgagatgcc gcagatgccg gtggcgccgc ccgagatcag gccgaagcgg gccggcaggc
      181 ccagcgcgcg cgccatcgcc aggccgaagc cgatggtcag cgccacggcg gcggccagcc
      241 aggccacgcc gctgccaccc agcgccagca cgtcaccgaa ggcgatgcgc gcgcccagca
      301 gggccacgcc caggcgcacc agctggcgcg ccgcgaactc gatgcccggg ccgcagcgcg
      361 cgtcttccga caggaagtgc agcgacatgc ccatcagcag ggcgtacagg aatttggggc
      421 cgccgtagtg ctcggacacg aaggtggcgg ccagcgcgac gatcagcgcc accagcaggc
      481 cgggcaccag gcggcgcgca cgtggccgcc agtcgcgcag ccgggccgcg gcgtcgacgt
      541 cagaaccttg ggacatcggg cgatgctagg ggtttctgct ttgtcgatcg atgattctgt
      601 aaatcagaaa tcaatcgaac gtcattccac cgtcacgctc ttggccaggt tgcgcggctt
      661 gtcgacgtcg gtgccgcgcg ccacggcggt gtggtaggcc agcagctgca gcggcaccac
      721 gtgcaggatg ggcgacagca ggcccgattg gccgggcatg cggatcacgt acaggccctc
      781 gctgctttcg atctgggtgt cggcgtcggc cagggcgtac agcacgccgc cgcgcgcgcg
      841 cacctcctgc atgttgctct tgagcttttc cagcagcgcg tcgttgggcg ccacggtgac
      901 caccggcatg gcgctggtca ccagcgccag cgggccgtgc ttgagctcgc cggcggcgta
      961 ggcctcggcg tggatgtagg tgatctcctt cagcttgagc gcgccttcct gggcgatcgg
     1021 gtagtgcagg ccgcggccca ggaacagcgc gtgctccttg gcggtgaact gttcggccca
     1081 gtcgatcagc tgcggctcca gcgccagcac cgcctgcagc gcgccgggca ggcggcgcag
     1141 cgcgtccagc tgctcggcct cctgcgccgg gccgagccga gcgcgcgtct tggccagcat
     1201 cagcgccagc aggaacaggc cgaccagctg ggtggtgaag gccttggtgc tggcgacgcc
     1261 gatctcgacc ccggcgcgcg tcagaaacgc cagccggcac tcgcgcacca tggcgctggt
     1321 ggcgacgttg cacaccgtca gcgtgtggcg catgcccagg ctttgcgcgt ggcgcagcgc
     1381 ggccagggtg tcggcggtct cgcccgactg gctgatggtg acgaccagcg tgtctgggtc
     1441 aggcaccgag gcgcggtagc ggtattcgct ggcgatctcg acctgggtcg ggatgccggc
     1501 gagcgcttcc agccagtact tggccacgca gccggcgtag tagctggtgc cgcaggccag
     1561 gatcagcacc tggcgcacgg cctggaaggt ggcctgcgcg tcctcgcccg cggccggctg
     1621 gccggcggcc tcgaacagct gcggcaccac ggcctgcacg ccctccagcg tgtcgcccac
     1681 ggcgcgcggc tgctcgaaga tctccttctg catgtagtgg cggtaaggcc ccagctcggc
     1741 cgcgccgctc atggcctgca cggtgcgcac cggccgctgc acgcgctggc cggcgcggtc
     1801 cagcacccag tacttgccca gctgcacgtc gatcagatcg ccctcttcca ggtagacgat
     1861 ctggtcggtc acgccggcca gcgccatggc gtcgctggcc aggaagttct ccttgccgtc
     1921 ggcaccgatg cccagcacca gcggcgagcc ctggcgcgcg cccaccaggc gccgcggctc
     1981 gtcgcggtgc agcacggcga tcgaatagct gccgtgcagc cgcttgaccg ccgcctgcac
     2041 cgccgccagc aagtcgccgt cgtacaggct gtcgatcagg tggacgatgg tctcggtgtc
     2101 ggtctggctg ctgaagtggt agccgcgtgc gcgcagctcg gcgcgcagcg cctcgtggtt
     2161 ctcgatgatg ccgttgtgca ccagggtgac gcggccgggc cgctcggcgc cgtcgt
//
LOCUS       DAOOOG010000305         2215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_61409_length_2215_cov_27.410185, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000305 DAOOOG010000000
VERSION     DAOOOG010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61409_length_2215_cov_27.410185"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PK242_13710"
     CDS             complement(<1..383)
                     /locus_tag="PK242_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95122.1"
                     /translation="MSRNDIIDVEPVGSARDDSLKTWGWVSYLLHLIVAVAAVVPGAQ
                     VSIALLIIALVLDLVKKDDAAGTWQASHFSWRIRSVLWAGVLYLVTFPLFLLFYLPGA
                     IAWVVISIWFLYRIVLGMVRMNAGRP"
     gene            complement(380..769)
                     /locus_tag="PK242_13715"
     CDS             complement(380..769)
                     /locus_tag="PK242_13715"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOZ95123.1"
                     /translation="MSHAELLAHLTAVIESRKPAAGGDPDTSYVARLLAKGPDAFLKK
                     IGEEATEVVMAAKDADHGGPRHKVVSEMADLWFHGMVALAHYGFSAADVVAELARREG
                     LSGLEEKALRKARQRDIDDASREGAGT"
     gene            complement(776..1168)
                     /gene="hisI"
                     /locus_tag="PK242_13720"
     CDS             complement(776..1168)
                     /gene="hisI"
                     /locus_tag="PK242_13720"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOZ95124.1"
                     /translation="MSNWLDQVAWDAQGLVPAIAQEADTGDVLMFAWMNREALARTAE
                     LGRAVYFSRSRGRLWFKGEESGHVQQVHEIRIDCDNDVVLLKVTQQGHRPGIACHTGR
                     HSCFFSVLKEGAWQAVEPVLKDPQSIYK"
     gene            complement(1440..>2215)
                     /locus_tag="PK242_13725"
     CDS             complement(1440..>2215)
                     /locus_tag="PK242_13725"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247444.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HOZ95125.1"
                     /translation="SVFTGKGIAYGGSLMRPEATGYGTVYFAQEMLRRKQMGFEDRRV
                     SISGSGNVAQYAADKAMELGAKVVTLSDSDGTLVCEGGLTHGMLQDLMEFKNVQRGRL
                     KDFCKQHKLKFEAGKRPWHVPVDIALPCATQNELDAADAKHLIANGVVCVAEGANMPS
                     TLDAVEHFLAAGTLYAPGKASNAGGVATSGLEMSQNAMRLSWTHSEVDLRLHEIMKDI
                     HGNCVRHGERKDGTVNYVEGANVAGFVKIADAMLAQGVC"
BASE COUNT          335 a          764 c          725 g          391 t
ORIGIN      
        1 gggcgcccgg cgttcatgcg caccatgccc agcacgatgc ggtagaggaa ccagatcgag
       61 atcaccaccc aggcgatcgc ccccggcagg tagaacagca ggaacagcgg gaaggtgacc
      121 aggtacagca cgccggccca cagcacggag cggatgcgcc agctgaagtg gctggcctgc
      181 caggtgccgg cggcatcgtc cttcttcacc aggtccagca ccagcgcgat gatcagcagc
      241 gcaatgctga cctgcgcgcc cggcaccacc gccgccaccg ccacgatcag gtgcagcagg
      301 tagctgaccc agccccaggt cttgagcgag tcgtcgcgtg cgctgcccac gggttccacg
      361 tcaatgatgt cgttgcggct catgttccag ctccttcgcg tgaagcgtca tcgatgtcgc
      421 gctggcgcgc cttgcgcagc gccttttctt ccaggcctga gagtccctcg cggcgcgcca
      481 gttccgccac cacgtcggcg gcggaaaacc cgtagtgcgc cagcgccacc atgccgtgaa
      541 accacaggtc ggccatctcg ctgaccacct tgtggcgcgg cccgccgtgg tcggcgtcct
      601 tggcggccat caccacctcg gtggcttctt cgccgatctt cttgaggaag gcgtcgggcc
      661 ccttggccag caggcgcgcg acgtagctgg tgtcggggtc gccgccggcc gccggcttgc
      721 ggctttcgat cacggcggtc agatgggcca gaagctcggc gtgcgacatg gggccttatt
      781 tatagatgga ctgggggtct ttcaagaccg gctcgacggc ctgccaggcg ccttccttca
      841 gcacgctgaa gaagcagctg tggcgcccgg tgtggcaggc aatgccgggc ctgtggccct
      901 gctgcgtcac cttgagcagc accacgtcat tgtcgcagtc gatgcggatt tcatgcacct
      961 gctgcacatg gccggactcc tcgcccttga accacaacct gccgcgcgaa cggctgaaat
     1021 acaccgcacg gccgagttcg gccgtcctgg ccagtgcttc gcggttcatc caggcgaaca
     1081 tcagcacgtc gcccgtgtcc gcttcctgcg cgatcgccgg caccaggccc tgcgcgtccc
     1141 aggcgacttg atccaaccag ttgctcatgc cgcgattgtc gggcattcgc ctggtggccg
     1201 cagaaactac aaaattgata gctgttggcg cttgatgggc aagcgccaga ggccgatttg
     1261 ccttgagccc aggttgaact ccgatggtgc cggtgatgcc cggcgccgac acgatgtccg
     1321 acctgcccct gatccaggcg ccgcgcggtc acccggcatg cgtcatcggg cacgcggccg
     1381 gagcccccgg cctcgggcga cgccccttga gggcgcaggc gcgccgcgcc aggaggtggt
     1441 cagcagacgc cctgcgccag catggcgtcg gcgatcttga cgaagcccgc cacgttggca
     1501 ccttcgacgt agttgacggt gccgtccttg cgctcgccgt ggcgcacgca gttgccgtgg
     1561 atgtccttca tgatctcgtg caggcgcaga tcgacctcgc tgtgggtcca ggacaggcgc
     1621 atggcgttct ggctcatctc caggcccgag gtggccacgc cgccggcgtt gctggccttg
     1681 cccggcgcgt acagggtgcc ggcggccagg aaatgctcga ccgcgtccag cgtgctgggc
     1741 atgttggcgc cttcggccac gcacaccacg ccgttggcga tcaggtgctt ggcgtcggcc
     1801 gcatcgagtt cgttctgggt ggcgcagggc agcgcgatgt ccaccggcac atgccagggg
     1861 cgcttgccgg cctcgaactt gagcttgtgc tgcttgcaga agtccttcag gcggccccgc
     1921 tgcacgttct tgaattccat caggtcctgc agcatgccgt gcgtcaggcc gccctcgcac
     1981 accagggtgc cgtcggagtc ggacagggtg accaccttgg cgcccagttc catcgccttg
     2041 tcggcggcgt actgcgccac gttgccggag cccgagatgc tgacgcgccg gtcttcgaag
     2101 cccatctgct tgcggcgcag catttcctgg gcgaagtaga ccgtgccata gccggtggcc
     2161 tcgggccgca tcaggctgcc gccgtaggcg atgcccttgc cggtgaacac gctgc
//
LOCUS       DAOOOG010000306         2206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_61801_length_2206_cov_55.641097, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000306 DAOOOG010000000
VERSION     DAOOOG010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61801_length_2206_cov_55.641097"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2206)
                     /locus_tag="PK242_13730"
     CDS             complement(<1..>2206)
                     /locus_tag="PK242_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ95126.1"
                     /translation="VKARLQTVTGVADVRIFGERKYAMRVWLDPERLAAYRLTTQDVE
                     DAIRRNNLELPAGRIESSQREFSVTSQTDLARPAQFADIVLRTVSGFPVRLRDVARVE
                     EGAADERSAVRLNGRSAISAGVIRQTTANPLLLSRGVRDMLPRLQEDLPSDVKIDIAN
                     DNSVFIDRAVSSVYTTITEAIVLVALVIFVFLRTVRASIIPIVTIPVSLIGAFALMSL
                     AGFSINTLTLLALVLAIGLVVDDAIVMLENIHRHIEEGMAPFAAAIHGAREIGFAIVA
                     MTLTLAAVYAPLAFTPGRTGRLFVEFALALAGAVLVSGFVALTLSPMMCSKLLRHNPR
                     PGMFDRGMERALMGLARAYGRLLRRVLTRARALVLLGMAGCAAAIAWLYPSMRQELSP
                     LEDRGVILANINAPDGATLDYTDRYARRLEAIGQQYPEFDRIFANIGNPSVSQGSVVY
                     RAVDWSERRRSTLDIARQMQPQFNQLAGVNAFPITPPSLGQGFRERPVNFVVQTSDSY
                     DNLNRAMRALMDEVAANPGFIAPDVDLRLSKPELRIEVDRERAADLGVSVDAVARAIE
                     TMLGGRQVTRYKRDAEQYDVIVQTTASGRGTPADIDRIQLRSTKGGVDTMIPLSSLVK
                     VHEGVSPRELNHFGQRRSATLTANLAPDYSLGQALAFLDEASTRVLGSGYTTDLNGIS
                     REFRSSQGALVIVFVLALLFIFLVLAAQFESFVDPLIIMFSVPLSMIGALLAL"
BASE COUNT          345 a          703 c          836 g          322 t
ORIGIN      
        1 agcgccagca gcgcgccgat catggacagc ggcaccgaga acatgatgat cagcgggtcg
       61 acgaagctct cgaactgcgc cgccagcacc aggaagatga acagcagcgc cagcacgaac
      121 acgatcacca gcgcgccctg gctggagcgg aattcgcgcg agatgccgtt caggtcggtg
      181 gtgtagccgc tgcccagcac gcgggtggag gcttcgtcga ggaacgccag cgcctggccc
      241 agcgagtagt cgggcgccag gttggccgtc agcgtggccg agcggcgctg gccgaagtgg
      301 ttgagctcgc gcggcgacac gccttcgtgc accttgacca ggctggacag ggggatcatg
      361 gtgtccaccc cgcccttggt gctgcgcagc tggatgcggt cgatgtcggc cggggtgccg
      421 cggccgctgg cggtggtctg cacgatgacg tcgtactgct cggcgtcgcg cttgtagcgg
      481 gtgacctggc gcccgcccag catggtttcg atggcgcgcg ccaccgcgtc gacgctgacg
      541 cccaggtcgg cggcgcgttc gcggtcgact tcgatgcgca gctcgggctt ggacaggcgc
      601 aggtcgacgt cgggggcgat gaagccgggg ttggcggcca cctcgtccat cagcgcgcgc
      661 atggcgcggt tgaggttgtc gtagctgtcg gaggtctgca ccacgaagtt gaccgggcgc
      721 tcgcgaaagc cctgccccag cgaaggcggc gtgatgggaa aggcgttaac gccggccagc
      781 tggttgaact gcggctgcat ctggcgcgcg atgtcgagcg tggagcgccg gcgctcgctc
      841 cagtcgaccg cgcggtacac cacgctgccc tgcgacacgc tggggttgcc gatgttggcg
      901 aagatgcggt cgaactcggg gtactgctgg ccgatggctt ccagccggcg cgcgtagcgg
      961 tcggtgtagt cgagcgtggc gccgtcgggt gcgttgatgt tggccaggat cacgcctcgg
     1021 tcttccagcg gcgacagctc ctgccgcatg ctggggtaga gccaggcgat ggcggcggcg
     1081 cagccggcca tgcccagcag caccagcgcg cgcgcgcgcg tgagcacgcg ccgcagcagg
     1141 cgaccgtagg cgcgcgccag gcccatgagg gcgcgctcca tgccgcggtc gaacatgccg
     1201 gggcgcgggt tgtggcgcag cagcttggag cacatcatgg gcgacagggt gagcgccacg
     1261 aagcccgaca ccagcaccgc gccggccagc gccagcgcga attcgacgaa cagccgcccg
     1321 gtgcgcccgg gggtgaaggc cagcggcgcg tacaccgccg ccagcgtgag cgtcatggcg
     1381 acgatggcaa agccgatttc gcgcgcgccg tggatggcgg cggcaaacgg cgccatgcct
     1441 tcttcgatgt ggcggtggat gttctccagc atgacgatgg cgtcgtccac caccaggccg
     1501 atggccagca ccagcgccag cagcgtgagc gtgttgatgg aaaagcccgc cagcgacatc
     1561 agggcgaagg cgccgatcag gctgaccggg atggtgacga tggggatgat ggaggcgcgc
     1621 accgtgcgca gaaagacgaa gatcaccagc gccaccagca cgatggcttc ggtgatggtg
     1681 gtgtagacgc tgctgacggc gcggtcgatg aacaccgagt tgtcgttggc gatgtcgatc
     1741 ttcacatcgg acggcaggtc ttcctgcagg cgcggcagca tgtcgcgcac gccgcgcgac
     1801 agcagcagcg ggttggcggt ggtctggcgg atcacgccgg ccgagatggc cgagcggccg
     1861 ttcaggcgca cggcgctgcg ctcgtcggcg gcaccctcct cgacccgggc gacgtcgcgc
     1921 aggcgcaccg gaaagccgct gacggtgcgc agcacgatgt cggcgaactg cgccggccgc
     1981 gccaggtcgg tctgcgaggt gacggagaac tcgcgctggc tggattcgat gcggccggcg
     2041 ggcagctcca ggttgttgcg gcggatggcg tcttcgacgt cctgcgtggt caggcggtag
     2101 gcggccaggc gctccgggtc cagccacacg cgcatggcgt acttgcgctc gccgaagatg
     2161 cgcacgtcgg ccacgccggt caccgtctgc aggcgcgcct tgacga
//
LOCUS       DAOOOG010000307         2206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_61802_length_2206_cov_28.260809, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000307 DAOOOG010000000
VERSION     DAOOOG010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61802_length_2206_cov_28.260809"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PK242_13735"
     CDS             complement(<1..749)
                     /locus_tag="PK242_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ95127.1"
                     /translation="MKRKTLCALITGLGAAFGGAAYAQISGGVVRIGVMNDMAGPYAD
                     LAGPGSVVAARMAVDDYLKATNSKLKVEIISADHQNKPDVGSSIARKWYDSDGVDMIL
                     DVPTSSVALAVNEVTKEKNKVMVNSTGGTADLTGKACTPNTVHWTYDTWMLAHGTGSA
                     VVKQGGDSWFFLTADYAFGHALERDTSDVVKASGGKVLGSVKVPLGTADFSSFLLQAQ
                     SSKAKIIGLANAGSDTINSIKQAAEFGIVKGG"
     gene            complement(829..1542)
                     /locus_tag="PK242_13740"
     CDS             complement(829..1542)
                     /locus_tag="PK242_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ95128.1"
                     /translation="MSHTSAPAIVLKDVHSWYGESHIMHGVHLEVKPGEVVTLLGRNG
                     AGRTTTLRAIMGLVGKRTGSIQVNGVETVKLAPHHIARLGLGYCPEERGIMTSLTCEE
                     NLMLPPKVAEGGMSVDEIYDMFPNLKARRNSPGGRLSGGEQQMLAVARILRTGAKVLL
                     LDEISEGLAPVIVQALARMIRELRARGFTVLMVEQNFRFAAPLADRFYIMERGLVLRE
                     FSAAELQDNMDMLHQYLGV"
     gene            complement(1556..>2206)
                     /locus_tag="PK242_13745"
     CDS             complement(1556..>2206)
                     /locus_tag="PK242_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ95129.1"
                     /translation="AGKTTCFNLLTKFLEPTRGQILFDGQDITAERPAQIARRGVVRS
                     FQISSTFPHMSVLENVRVSLQQKLGTSFHFWKSERTLDQLNERCMELLGFVGLADFAQ
                     LEAVGLPYGRKRALEIATTLAMDPKLMLLDEPTQGMGHEDVDMVTQLIKKVAANRTVL
                     MVEHNMSVVGSIADQITVLQFGQVIAEGPYAEVSRNPQVLEAYMGSAEEALQGAHG"
BASE COUNT          336 a          710 c          752 g          408 t
ORIGIN      
        1 ccgcccttga cgatgccgaa ctcggccgcc tgcttgatcg agttgatggt gtcgctgccg
       61 gcgttggcca ggccgatgat cttggccttg gacgactgcg cctgcagcag gaaggaggag
      121 aagtcggccg tgcccagcgg caccttgacg ctgcccagca ccttgccgcc ggaggccttg
      181 accacgtcgg aagtgtcgcg ctccagggcg tggccgaagg cgtagtcggc ggtcaggaaa
      241 aaccagctgt cgccgccctg cttgaccacc gccgagccgg tgccgtgggc cagcatccag
      301 gtgtcgtagg tccaatgcac ggtgttgggc gtgcaggcct tgccggtcag gtcggcggtg
      361 ccgccggtgg agttgaccat caccttgttc ttttccttgg tgacttcgtt gaccgccagc
      421 gccaccgacg atgtgggcac gtccaggatc atgtcgacgc cgtccgagtc gtaccacttg
      481 cgggcaatgc tggagccgac gtcgggcttg ttctgatggt cggcggagat gatttccacc
      541 ttgagtttgg agttggtggc cttgaggtag tcgtccaccg ccatcctggc ggcgaccacc
      601 gaaccggggc cggccaggtc ggcatagggg ccggccatgt cgttcatcac gccgatcctg
      661 acgacgccgc ccgagatctg ggcgtacgcg gcgccgccga acgcggcgcc caggccggtg
      721 atcagggcgc acagtgtctt gcgtttcatg gggtgtgttc ctctgggggc aaagaagtca
      781 gatcgatcgg cggcaggccc ggggttccat aggggcggtg gtcacggctc acacgcccag
      841 gtactggtgc agcatgtcca tgttgtcctg cagttcggcg gcgctgaact cgcgcagcac
      901 cagcccgcgc tccatgatgt agaagcgatc ggccagcggt gcggcgaagc ggaagttctg
      961 ctccaccatc agcaccgtga agccgcgcgc gcgcagctcg cggatcatgc gcgccagcgc
     1021 ctgcacgatg accggcgcca ggccttcgga gatctcgtcc agcagcagca ccttggcgcc
     1081 ggtgcgcaga atgcgcgcca ccgccagcat ctgttgttcg ccgcccgaca gccggccgcc
     1141 cgggctgttg cgcctggcct tcaggttggg gaacatgtcg tagatctcgt ccacgctcat
     1201 gccgccttcg gccaccttgg gtggcagcat caggttttcc tcgcaggtca gcgaggtcat
     1261 gatgccgcgc tcttccgggc aatagcccag gcccaggcgg gcgatgtggt gcggcgccag
     1321 cttgacggtt tcgaccccgt tgacctggat cgacccggtg cgcttgccca ccaggcccat
     1381 gatggcacgc agggtggtgg tgcgaccggc accgttgcgg cccagcaggg tgaccacctc
     1441 gcccggcttc acctccaggt ggacgccgtg catgatgtgc gattcgccgt accaggaatg
     1501 gacgtccttg aggacgatgg cgggagcgga tgtgtggctc atgcggtgac tgtcctcaac
     1561 catgcgcgcc ctgcagggct tcttcggcgc tgcccatgta ggcttccagc acctgcgggt
     1621 tgcgcgacac ctcggcgtac ggcccttccg cgatgacctg accgaactgc agcacggtga
     1681 tctggtcggc gatgctgccc accacgctca tgttgtgctc caccatcagc acggtgcggt
     1741 tggcggccac cttcttgatc agctgggtga ccatgtcgac gtcctcgtgg cccatgccct
     1801 gggtgggctc gtccagcaac atcagcttgg gatccatcgc cagcgtggtg gcgatctcca
     1861 gcgcgcgctt gcggccatag ggcaggccga ccgcctccag ctgggcgaag tcggccaggc
     1921 cgacgaagcc cagcagctcc atgcagcgct cgttgagctg gtccagggta cgttcggact
     1981 tccagaagtg aaacgaggtg cccagcttct gttgcaggga cacgcgcacg ttttccagca
     2041 cgctcatgtg cgggaaggtg gacgaaatct ggaacgagcg caccacgccg cggcgggcga
     2101 tctgggccgg ccgctcggcg gtgatgtcct gcccgtcgaa caggatctgg ccgcgcgtgg
     2161 gctccaggaa cttggtcagc aggttgaagc aggtggtctt gccggc
//
LOCUS       DAOOOG010000308         2197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_62182_length_2197_cov_24.519608, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000308 DAOOOG010000000
VERSION     DAOOOG010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62182_length_2197_cov_24.519608"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /gene="sbcB"
                     /locus_tag="PK242_13750"
     CDS             complement(<1..653)
                     /gene="sbcB"
                     /locus_tag="PK242_13750"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HOZ95130.1"
                     /translation="MSASTFLWHDYETFGTRPRRDWPAQFAAVRTDAELNEIGEPLVL
                     YCQPPTDRLPEPEACLITGITPQECAAKGLPEHEFARRIHAALAQPGTIGVGYNSIRF
                     DDEVTRFLLWRNLIEPYGREWRDGCSRWDLLDVMRCAHALRPEGIEWPQGEDGLTTFK
                     LERLSAANGLAHEAAHDALSDVRATVALARLLRQCQPRLFDFCLALRSKDRVAQELGL
                     "
     gene            781..1758
                     /locus_tag="PK242_13755"
     CDS             781..1758
                     /locus_tag="PK242_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOZ95131.1"
                     /translation="MNTPTFAADRRAHHLIVGDGVVGRATAQALHAQGQRVVLASRHA
                     PAAGAAEATHLTVDALDAPSLRRAAEGASHLYLTLGLAYRIGVWQRDWPRIMHNAIQA
                     ALASGARLLWFDNVYLYGTPLQVPMRENHPIDPPSHKGRVRARLLAMLLEAEDRHGLR
                     WLVARSADFYGPDVRLSILYAAAMERQLQRRAAWWLGDPDRRHSFTYVPDAGRALARL
                     ALDDGTWQQSWHLPTASPAPTPRELLTESARLLGAPTEVRALPVALVRTLGLAVPLLR
                     EVGEMLYQNQQDYVFSSEKFMARYPDFRITPYAEGLTAMIASLRPAART"
     gene            complement(1817..>2197)
                     /gene="clpA"
                     /locus_tag="PK242_13760"
     CDS             complement(1817..>2197)
                     /gene="clpA"
                     /locus_tag="PK242_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901510.1"
                     /note="ATPase and specificity subunit of the ClpA-ClpP ATP
                     dependent serine protease; directs protease to specific
                     substrates; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HOZ95132.1"
                     /translation="LDAIVSFKPLDEQVILRVVDKFLLQLEQQLAEKKVEVTFTDKLR
                     KFLAKKGFDPLMGARPMQRLIQDTIRKALADELLFGRLTEGGRLTVDLDDKDEVLLDI
                     TPLPKKERARGAEPAEPEEEIATD"
BASE COUNT          320 a          776 c          764 g          337 t
ORIGIN      
        1 aggcccagct cctgggccac gcggtccttg ctgcgcaggg ccaggcagaa gtcgaacaac
       61 ctgggctggc actggcgcag caggcgcgcc agcgccacgg tggcgcgcac gtcggacagc
      121 gcgtcgtgcg ccgcttcgtg cgccagaccg ttggcggcgc tcaggcgctc cagcttgaag
      181 gtggtcaggc cgtcctcgcc ctgcggccac tcgatgccct cgggtcgcaa ggcgtgggcg
      241 cagcgcatca cgtccagcag gtcccagcgg ctgcaaccgt cgcgccactc gcggccatag
      301 ggctcgatca ggttgcgcca gagcagaaag cgcgtcacct cgtcgtcgaa gcggatcgag
      361 ttgtagccca cgccgatggt gcccggctgg gccagggcgg cgtggatgcg gcgggcaaat
      421 tcgtgctcgg gcagcccctt ggcggcgcac tcctgcggcg tgatgccggt gatcaggcag
      481 gcctcgggct cgggcaggcg gtcggtcggc ggctggcagt acagcaccag cggctcgccg
      541 atctcgttca gctcggcatc ggtgcgcacg gcggcgaact gggccggcca gtcgcggcgc
      601 gggcgggtgc cgaaggtttc gtagtcgtgc cagaggaagg tggaggcgct catggggtgg
      661 caagcataat ggccagcccg gctgcgcgcc ccctggcggg tgcgaggcct gatgcatctt
      721 ggaacgatga ctttgtccca cagcatgccg ccgccacctc cgacggttca tgatggcgac
      781 atgaacaccc ccaccttcgc cgccgatcgc cgcgcgcacc atctgatcgt gggcgatggc
      841 gtggtcggcc gcgccaccgc acaggcgctg cacgcgcagg gccagcgggt ggtgctggcc
      901 agccgccatg cgcctgcggc aggtgccgcc gaggccaccc acctgacggt cgacgcgctg
      961 gacgcgcctt ccctgcgccg tgccgccgag ggcgccagcc acctgtatct gacgctcggc
     1021 ctggcctatc ggatcggcgt gtggcagcgc gactggccgc gcatcatgca caacgccatc
     1081 caggcggcgc tggccagcgg cgcgcggctg ctgtggttcg acaacgtcta cctctacggc
     1141 acaccgctgc aggtaccgat gcgggaaaac catccgatcg atccaccgtc gcacaagggc
     1201 cgcgtgcggg cgcgcctgct ggccatgctg ctggaggccg aggatcgcca tggcctgcgc
     1261 tggctggtgg cacgcagcgc cgacttctat ggcccggacg tgcgcctgtc gatcctgtac
     1321 gcggccgcca tggagcgcca gttgcaaagg cgcgcggcat ggtggctggg cgaccccgac
     1381 cggcgccaca gcttcaccta cgttccggac gccggccgcg cgctggcgcg cctggccctg
     1441 gatgacggca cctggcagca aagctggcac ctgcccaccg ccagcccggc accgacgccc
     1501 cgcgaactgc tgaccgaaag cgcacgcctg ctgggggcgc ccaccgaagt gcgcgcccta
     1561 ccggtggccc tggtgcgcac gctcgggctg gccgtgccgc tgctgcgcga agtcggtgaa
     1621 atgctttacc agaaccagca ggactacgtc ttcagcagcg agaagttcat ggcgcgctac
     1681 cccgactttc gcatcacgcc ctacgccgag gggctgacgg cgatgatcgc ctcgctgcgc
     1741 cccgcggcgc gcacctgaac ccacaaaaaa gcccgctgcg tggcagcggg ctggtcgggt
     1801 gaacgcaggc tcgacctcag tcggtggcga tctcttcttc cggctcggcc ggctcggcgc
     1861 ccctggcacg ctccttcttc ggcagcggcg tgatgtcgag cagcacctcg tccttgtcgt
     1921 ccaggtccac cgtcaggcgc ccgccctcgg tcaggcggcc gaacagcagc tcgtcggcca
     1981 gcgccttgcg gatggtgtcc tgaatcaggc gctgcatcgg gcgcgcgccc atgaggggat
     2041 cgaagccctt cttggccagg aatttgcgca gcttgtcggt gaaggtgact tcgaccttct
     2101 tctcggccag ctgctgctcc agctgcagca ggaacttgtc gaccacgcgc aggatgacct
     2161 gctcgtccag cggcttgaag ctgacgatgg cgtccag
//
LOCUS       DAOOOG010000309         2187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_62609_length_2187_cov_26.147749, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000309 DAOOOG010000000
VERSION     DAOOOG010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62609_length_2187_cov_26.147749"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PK242_13765"
     CDS             complement(<1..526)
                     /locus_tag="PK242_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ95133.1"
                     /translation="MSKIDFNGRVAIVTGAGGGLGRLHALALAERGARVVVNDLGGAV
                     DGSGGSATAAERVVEEIRARGGQAIASGASVTDAQAMQAMVQQALDTWGRVDVLVCNA
                     GILRDKSFAKMDLADFRLVLDVHLMGAVHLVKAAWPHLVAQKYGRILMTTSSTGLYGN
                     FGQSNYGAAKLALVG"
     gene            653..1543
                     /locus_tag="PK242_13770"
     CDS             653..1543
                     /locus_tag="PK242_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOZ95134.1"
                     /translation="MELNHETIDTPPLDAASVVMLRDGARGLEVLLLRRHANSSVLGG
                     AYVFPGGKVDAADASADTLALLDRAPTELHARLGEPALTPAQAAAFYVAAAREAAEES
                     GVLYLQVGADEVARHSAATCARLRAGEPFAALLAEHGALLDSDALAPWSRWITPRRPS
                     VMNKRFDTRFFLAGVPPAQQARHDDFEVTEARWLTPREALRQYREHQIDMAPPQIVSL
                     LHLLHYRDLAHAMQDARTRRPPLIEPEPIEQGDTRVLCYPGDAAHSVAARALPEPVPT
                     RVVWRGKRFEPPQGFGPFLD"
     gene            1641..>2187
                     /locus_tag="PK242_13775"
     CDS             1641..>2187
                     /locus_tag="PK242_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ95135.1"
                     /translation="MHPAVRRQTIADLLRRTAQRLPAKLAVQCGATRWSYAEFDALVT
                     RLAAGLAGRGVAHGDRVAILARNSHGFAAMRFALARLGAVLVPINFMLKADEVAFILR
                     HAGAKLLATDSGLAELARASAALDTQVTDFVWLPSEDPSEPVAGMASFHDLAACTAPL
                     PDTPLASTDLAQIVYTSGTESS"
BASE COUNT          316 a          827 c          741 g          303 t
ORIGIN      
        1 ggccgaccag cgccagcttg gcggcgccgt agttgctctg gccgaagttg ccgtagagac
       61 cggtggacga ggtggtcatc aggatgcgcc cgtatttctg cgccaccagg tgcggccagg
      121 ccgccttgac caggtgcacc gcgcccatca ggtgcacgtc cagcaccagg cggaagtcgg
      181 ccagatccat cttggcaaag ctcttgtcgc gcaggatgcc ggcgttgcac accagcacat
      241 cgacccggcc ccaggtatcc agcgcctgct gcaccatggc ctgcatggcc tgggcatcgg
      301 tcaccgaagc gccgctggcg atcgcctggc cgccgcgggc gcggatttcc tcgaccacgc
      361 gctcggccgc ggtcgccgag ccgccgctgc cgtccaccgc gccgcccagg tcgttgacca
      421 ccaccctggc gccgcgctcg gccagcgcca gcgcgtgcag gcgccccagg ccgccgccgg
      481 cgccggtgac gatggccacg cggccgttga agtcgatctt gctcatgctg tgctcactcc
      541 tgttgaaaga atcccgtcga tgccgactgt aaccaaccgg ccaggccgta tgcgcccgtg
      601 ggcggacgca ggtgacagcg gcgccccgcg gccccggttc acaatgcgcg ccatggaact
      661 gaaccacgaa accatcgaca ccccgccgct cgacgcagcc agcgtggtga tgctgcgcga
      721 cggcgcccgg ggactggaag tgctgctgct gcgccgccac gccaactcgt cggtgctggg
      781 cggcgcctac gtgtttcccg gcggcaaggt ggacgcggcc gatgccagcg ccgacacgct
      841 ggccctgctg gaccgcgcgc cgaccgagct gcatgcgcgc ctgggcgagc cggcgctgac
      901 gccggcgcag gcggcggcgt tctacgtcgc tgcggcacgc gaggcggccg aggaatcggg
      961 cgtgctgtac ctgcaggtgg gcgccgacga ggtggcccgc cacagcgccg ccacctgcgc
     1021 gcgcctgcgc gccggcgaac cgtttgccgc cctgctggcc gagcacggcg cgctgctgga
     1081 cagcgacgcc ctcgccccct ggtcgcgctg gatcacgccg cgccggccct cggtgatgaa
     1141 caagcgcttc gacacgcgct ttttcctggc cggcgtgccg ccggcgcagc aggcgcgcca
     1201 cgacgacttc gaggtcacgg aagcgcgctg gctgacgcca cgcgaggcgc tgcggcaata
     1261 ccgcgagcac cagatcgaca tggcgccgcc gcagatcgtc agcctgctgc acctgctgca
     1321 ctaccgcgac ctggcgcatg ccatgcagga tgcgcgcacg cgccgcccac cgctgatcga
     1381 gccggaaccg atcgagcagg gcgacacgcg cgtgctgtgc taccccggcg acgcggcgca
     1441 cagcgtcgcg gcacgcgcgc tgcccgagcc ggtgcccacg cgcgtggtgt ggcgaggcaa
     1501 gcgtttcgag ccgccacagg gtttcgggcc gttcctggac tgagccaaga caccctgaga
     1561 aactatcaaa ttaatagctg ctggcgcttg ccccgactgc ctcagcgccc gaaatgccat
     1621 cccaaaccgg agacctcccg atgcatcccg ccgtccgccg ccagaccatc gccgatctgc
     1681 tgcgccgcac cgcccagcgc ctgcccgcca agctggcggt gcagtgcggc gccacgcgct
     1741 ggagctatgc cgagttcgac gccctggtca cccgcctggc cgccggcctg gccggccgcg
     1801 gggtggcgca tggcgaccgg gtggccatcc tggcgcgcaa ttcgcacggc tttgccgcca
     1861 tgcgctttgc cctggcgcgc ctgggcgcgg tgctggtgcc gatcaacttc atgctgaagg
     1921 ccgacgaggt ggcgttcatc ctgcgccacg ccggcgccaa gctgctggcc accgacagcg
     1981 ggctggccga gttggcgcgc gcttccgccg cactggacac gcaggtgacg gacttcgtgt
     2041 ggctgccttc ggaagacccg agcgaaccgg tggcgggcat ggccagcttt cacgacctgg
     2101 ccgcctgcac cgcgccgctg cccgacacgc cgcttgccag caccgacctg gcgcagatcg
     2161 tctacaccag cggcaccgag tccagcc
//
LOCUS       DAOOOG010000310         2158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_63828_length_2158_cov_28.016643, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000310 DAOOOG010000000
VERSION     DAOOOG010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63828_length_2158_cov_28.016643"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PK242_13780"
     CDS             <1..459
                     /locus_tag="PK242_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ95136.1"
                     /translation="FAAAVLAHMRAGSQIAFIASLAALTGNFGYTAYCASKYGVRGLA
                     EALRYELKLQGINVSLCCPGEIMTPMVAQEKQHMHPVSAAMKAFAGSSTLDASVPALL
                     RGIARREFEITDTPKAALTAFLSRHLPGLARAVGDRIARSAQQKLQKGQP"
     gene            456..1934
                     /locus_tag="PK242_13785"
     CDS             456..1934
                     /locus_tag="PK242_13785"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HOZ95137.1"
                     /translation="MTANPPSTAWKAMHIPAHLPANLRDCFAAQQAAFAQNRYPSLAE
                     RRADLRALHRLLVENRDALVDAVNRDFGCRSHFETLFAELLQGQEGALDAIRRLPRWM
                     KRQRRHLDMTQYPLASAFVQPQPLGVVGIVAPWNFPIAMVVQPLIGALAAGNRAMVKM
                     SENSERLARLLQELLPKYFAADKVSLFADSDAGPGQSLGPLFTQLPFNHLFFTGSPQT
                     GKAVMANCAANLTPVTLELGGKSPAIVAPGYAMHKAAERILWVKMLNAGQICTNVDYM
                     FVPAGQEQAFIEAAQRIVAQRYPNLLNGDYTAIIDQRQYDRLQAMLADAVAQGAQVVP
                     LAPGQQGDAARRIMPPVALLNVHDGMQVMQREIFGPLLPILSYQTHDEVLHYINDRPH
                     PLALYLFSDDKALQQQYLQQTLSGGVTLNDTLLHVGQHSLPFGGVGNSGMGHYHGHEG
                     FLTFSKLRPVFRQGPLRTVDFLMPPYRGMACRMLDVMFWRNR"
     gene            2004..>2158
                     /locus_tag="PK242_13790"
     CDS             2004..>2158
                     /locus_tag="PK242_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOZ95138.1"
                     /translation="MNGAQALIKTLADAGVEVCFSNPGTSEMHFVAALDSEPRMRAVL
                     ALFEGVAT"
BASE COUNT          372 a          785 c          663 g          338 t
ORIGIN      
        1 ttcgccgccg ccgtgctggc gcacatgcgc gcgggcagcc agatcgcctt catcgcgtcc
       61 ctcgccgcgc tcacgggcaa ctttggctac acggcctatt gcgcgtccaa gtacggcgtg
      121 cggggcctgg ccgaggcgct gcgctatgag ctgaagctgc agggcatcaa cgtgagcctg
      181 tgctgccccg gcgagatcat gacccccatg gtggcgcagg aaaagcagca catgcacccc
      241 gtgtcggcgg ccatgaaggc ctttgccggc agcagcacgc tcgacgcctc cgtgcccgcg
      301 ctcttgcgcg gcattgcgcg gcgtgagttc gagattaccg acacacccaa ggcggcgctg
      361 accgccttcc tctcgcgcca cctgcccggc ctggcgcgtg ccgtgggcga ccgcattgcc
      421 cgcagcgccc aacagaaact gcagaaagga cagccatgac cgccaacccg cccagcaccg
      481 cctggaaggc catgcacatc cccgcacacc tgcccgccaa tctgcgcgac tgctttgccg
      541 cgcagcaggc ggcctttgcc cagaaccgct accccagcct ggccgagcgc cgcgccgacc
      601 tgcgcgcgct gcaccgcctg ctggtcgaaa accgcgacgc gctggtcgat gccgtgaacc
      661 gcgactttgg ctgccgcagc catttcgaaa ccctgtttgc cgagctgctg cagggccagg
      721 aaggagcgct cgatgcgatc cgccggctgc cgcgctggat gaaacgccag cgccgccatc
      781 tggacatgac gcagtacccg ctggccagcg cctttgtcca gccccagccg ctgggcgtgg
      841 tcggcatcgt ggcgccgtgg aacttcccca tcgccatggt ggtgcagccg ctcataggcg
      901 cgctggccgc gggcaaccgc gccatggtca agatgtcgga aaactccgaa cgcctcgcgc
      961 gcctgctgca agagctgttg cccaaatact ttgccgccga caaggtgagc ctctttgccg
     1021 acagcgacgc ggggccgggc cagtcgctcg ggccgctgtt cacgcagctg ccgttcaacc
     1081 acctgttctt caccggctcg ccgcaaaccg gcaaggccgt gatggccaac tgcgcggcca
     1141 acctcacgcc cgtcacgctg gagctgggcg gcaagtcgcc cgccatcgtc gcgcccggct
     1201 acgccatgca caaggcggcc gagcgcattc tgtgggtcaa gatgctcaac gccgggcaga
     1261 tctgtaccaa tgtggactac atgttcgtgc ccgccggcca ggagcaggcc ttcatcgaag
     1321 cggcgcagcg catcgtcgcc cagcgttacc ccaatctctt gaacggcgac tacaccgcca
     1381 tcatcgacca gcgccagtac gaccgcctgc aggccatgct ggccgatgcg gtagcccagg
     1441 gtgcccaggt cgtgccgctg gcccccggcc aacagggcga tgcggcgcgg cgcatcatgc
     1501 ctccggtggc gctgctgaac gtgcacgacg gcatgcaggt catgcagcgc gagatcttcg
     1561 gcccgctgct gcccatcctc agctaccaaa cccacgacga ggtgctgcac tacatcaacg
     1621 accggccgca cccgctggcg ctctacctgt tcagcgacga caaggcgctg cagcagcagt
     1681 atctgcagca aacgctgtcg ggcggcgtca cgctcaacga cacgctgttg cacgtgggcc
     1741 agcacagtct gccctttggc ggcgtgggca acagcggcat ggggcactac cacggccatg
     1801 aagggttcct gaccttctcc aagctgcgcc cggtgttccg ccaggggccg ctgcgcaccg
     1861 tggacttttt gatgccaccc taccggggca tggcgtgcag gatgctggac gtcatgttct
     1921 ggcgcaaccg ctgaaagttt gaataagaac cggctccagc gcccacccat caagcgccag
     1981 cagctatgaa tcaaggagta cccatgaacg gcgcacaagc cctgatcaag accctggccg
     2041 acgccggcgt cgaagtatgt ttttccaacc ccggcaccag cgagatgcac ttcgtcgccg
     2101 cgctcgactc cgagccgcgc atgcgcgccg tgctcgcgct gttcgagggc gtggccac
//
LOCUS       DAOOOG010000311         2143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_64484_length_2143_cov_59.707375, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000311 DAOOOG010000000
VERSION     DAOOOG010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64484_length_2143_cov_59.707375"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /gene="rpsE"
                     /locus_tag="PK242_13795"
     CDS             complement(<1..413)
                     /gene="rpsE"
                     /locus_tag="PK242_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOZ95139.1"
                     /translation="MASKFQARAQGDGPEDGLREKMIAVNRVTKVVKGGRILGFAALT
                     VVGDGDGRVGMGKGKSKEVPSAVQKAMEEARRNMLKVSLKDGTIHHNVTGHHGAATVM
                     MAPAPRGTGIIAGGPMRAVFEVLGITDIVAKSHGTS"
     gene            complement(426..791)
                     /gene="rplR"
                     /locus_tag="PK242_13800"
     CDS             complement(426..791)
                     /gene="rplR"
                     /locus_tag="PK242_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOZ95140.1"
                     /translation="MTTKKEQRLRRARQTRIRIADKGVCRLSVNRTNLHIYATVFSAD
                     GTRVLATASTAEAEMRQALGAAGKGGNVAAAQQIGKRIAERAKAAGVDKVAFDRAGFA
                     YHGRVKALAEAAREAGLQF"
     gene            complement(803..1336)
                     /gene="rplF"
                     /locus_tag="PK242_13805"
     CDS             complement(803..1336)
                     /gene="rplF"
                     /locus_tag="PK242_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOZ95141.1"
                     /translation="MSRIAKMGVTVPAGVDVQIKEDQIMVKGSGGELKLAPSALVKIA
                     MADGKLSFEPADESREANAMSGTMRQLVNNMVVGVSKGFEKKLTLVGVGFRAQAQGSK
                     LNLAIGFSHPVNFDMPAGVTVATPTPTEIVIKGANRQVVGQIAAEIRDVRPPEPYKGK
                     GIRYADERVVIKETKKK"
     gene            complement(1349..1744)
                     /gene="rpsH"
                     /locus_tag="PK242_13810"
     CDS             complement(1349..1744)
                     /gene="rpsH"
                     /locus_tag="PK242_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOZ95142.1"
                     /translation="MSMSDPIADLLTRIRNAQMVAKQTVSVPSSKVKVAIAQVLKDEG
                     YIDGFEVKSEGGKSELEIALKYYAGRPVIERIERVSRPGLRVYKGRDAIPQVMNGLGV
                     AIVTTPRGVMTDRKARATGVGGEVLCYVA"
     gene            complement(1763..2068)
                     /gene="rpsN"
                     /locus_tag="PK242_13815"
     CDS             complement(1763..2068)
                     /gene="rpsN"
                     /locus_tag="PK242_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOZ95143.1"
                     /translation="MAKVALIERELKREKLVAKYAKKHAELKAIAGDAKRSDDERAQA
                     RLALQKLPRNANPTRQRNRCEMTGRPRGTFRQFGLGRAKIRELAFEGQIPGVTKASW"
BASE COUNT          293 a          713 c          698 g          439 t
ORIGIN      
        1 gacgtgccgt ggctcttggc cacgatgtcg gtgatgccca gcacctcaaa cacggcgcgc
       61 atcgggccgc cggcgatgat gccggtaccg cgcggcgccg gcgccatcat gacggtggcg
      121 gcgccgtggt ggccggtcac gttgtggtga atggtgccgt ccttgagcga caccttcagc
      181 atgttgcgac gcgcctcttc catggccttc tggacggccg acggcacttc cttggatttg
      241 cccttgccca tgccgacgcg gccatcgccg tcgcccacca cggtcagtgc ggcaaaaccc
      301 aggatgcggc cacccttgac caccttggtg acgcggttga ccgcgatcat cttctcgcgc
      361 aggccgtcct cgggaccatc gccctgcgcg cgtgcttgaa acttgcttgc catctcgttg
      421 gtcctttaga actgcaggcc cgcttcgcgc gcggcttcgg ccagcgcctt gacacggccg
      481 tggtaggcga aaccggcgcg gtcgaacgcc accttgtcga caccggcggc cttggcgcgc
      541 tcggcgatgc gcttgccgat ctgctgcgcg gcggccacgt tgccaccctt gccggcggcg
      601 cccagcgcct gacgcatctc ggcttcggcg gtcgaggccg tggccagcac gcgcgtgcca
      661 tcggccgaga acacggtggc gtagatatgc aggttggtgc ggttcacgct caggcggcac
      721 acacccttgt cggcgatgcg gatgcgcgtc tggcgggcac ggcgaaggcg ctgctctttc
      781 ttggtcgtca tgatgcaggt ccttacttct tcttggtttc cttgatgacg acgcgctcgt
      841 ccgcgtagcg gatgcctttg cccttgtagg gctcgggcgg acgcacgtca cgaatctcgg
      901 ccgcgatctg gcccaccacc tggcggttgg cgcccttgat cacgatttcc gtcggcgtcg
      961 gcgtggccac cgtcacgccg gccggcatgt cgaagttgac cggatgcgag aagccgatcg
     1021 ccaggttcag cttgctgccc tgggcctggg cgcgaaagcc cacgccgacc agggtgagct
     1081 tcttctcgaa acccttgctg acgccgacca ccatgttgtt gaccagctgg cgcatggtgc
     1141 cgctcatggc gttggcctcg cgcgactcgt cggccggctc gaacgacagc ttgccgtcgg
     1201 ccatggcgat cttcaccagg gcgctggggg ccagcttgag ctcgccgccg ctgcccttga
     1261 ccatgatctg gtcttccttg atctgaacat ccacgcccgc ggggacggtg acgcccattt
     1321 ttgcaatacg cgacatttcg atttctcctt acgccacgta gcacagcact tcgccaccga
     1381 caccggtggc gcgcgccttg cgatcggtca tcacgccgcg cggggtggtg acgatggcca
     1441 cgcccaggcc attcatgacc tggggaatgg cgtcacggcc cttgtagacg cgcaggccgg
     1501 gacggctgac gcgctcgatg cgctcgatca ccgggcggcc ggcgtagtat ttgagggcga
     1561 tttccagttc ggacttgccg ccttcgctct tgacttcgaa gccgtcgatg tagccctcgt
     1621 ccttcaggac ctgggcgatg gccaccttga ccttggacga cggcaccgac acggtctgct
     1681 tggccaccat ctgcgcattg cggatgcggg tcagcagatc ggcgatggga tcactcatgc
     1741 tcatgttgct tactcctgcc gattaccagc tggccttggt gacgccggga atctggcctt
     1801 cgaaggccag ttcgcggatc ttggcgcggc cgagaccgaa ctgacggaag gtgccgcgcg
     1861 ggcggccggt catttcgcag cggttgcgct ggcgcgtggg gttggcgttg cgcgggagct
     1921 tctgcagcgc cagacgggcc tgcgcgcgct cgtcgtcgct gcgcttggcg tcgccggcga
     1981 tggccttcag ctcggcgtgc ttcttggcgt acttggcgac cagtttctcg cgcttgagtt
     2041 cgcgctcgat caaagctact tttgccatgg ccttacctca gttcttgaac gggaaacgga
     2101 aagccgccag cagcgccttg gcttctgcgt cggtcttggc cgt
//
LOCUS       DAOOOG010000312         2141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_64568_length_2141_cov_22.028284, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000312 DAOOOG010000000
VERSION     DAOOOG010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64568_length_2141_cov_22.028284"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PK242_13820"
     CDS             complement(<1..302)
                     /locus_tag="PK242_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOZ95144.1"
                     /translation="MSSTTHGQTPYYYVPAESRHPVMAAFGLLLMIIGASQWINDAAW
                     GGWLLLAGLLWWLFVLFQWFSDAVGESEGGLYGRKIDLSYRWSMSWFIFSEVMFFGA"
     gene            complement(392..667)
                     /locus_tag="PK242_13825"
     CDS             complement(392..667)
                     /locus_tag="PK242_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HOZ95145.1"
                     /translation="MSEPARTVQTPVSPAPPDVAAPAAAGGPAFWRTLRMVGWGFLGV
                     RKRSEYQRDLAQVNPFHIMLAGLIGVVLFVLLLLGIIHWVVSGTTPG"
     gene            complement(664..1281)
                     /locus_tag="PK242_13830"
     CDS             complement(664..1281)
                     /locus_tag="PK242_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOZ95146.1"
                     /translation="MRRRSENLKMLGKLAVVACGMFAFGYSLIPVYRAICEATGINIL
                     SLSERQVPGNGSAGETARLASRNTQVDRSRVITVEFDANTRGPWEFRPAQRTLQVHPG
                     ELATVMYEFQNVQDRRMAAQAIPSYAPRQAAPHFNKLECFCFNQYTLEPGEKKEWPVA
                     FVIDPRLPKDVTTITLSYTFFEVGGKTPAPPQSSAALARPHGAGA"
     gene            complement(1297..1410)
                     /locus_tag="PK242_13835"
     CDS             complement(1297..1410)
                     /locus_tag="PK242_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HOZ95147.1"
                     /translation="MTTEQQRKRNVRLGLILGTVALAFFVGFIVRMVVLGG"
     gene            complement(1437..>2141)
                     /locus_tag="PK242_13840"
     CDS             complement(1437..>2141)
                     /locus_tag="PK242_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOZ95148.1"
                     /translation="HHMFTTGMPVTGQLFFMYGTMLIAIPTGVKIFNWVATMWRGSMT
                     FETPMLWAVGFIFVFTIGGFTGVIPAVVPVDTQIQDTYYIIAHFHYVLVAGSLFALYG
                     GFYYWSPKWTGVMYNETRGKIHFWWSMISFNVTFFPMHFLGLAGMPRRYADYPMQFAD
                     FNLIASVGAFFFGFAQLYFFLFVVLPVMRGQGAKAPQRPWEGAEGLEWEVPSPAPFHT
                     YETPPKLDASATRVIG"
BASE COUNT          396 a          700 c          710 g          335 t
ORIGIN      
        1 gcgccgaaga acatcacctc cgagaagatg aaccagctca tgctccagcg gtaggacagg
       61 tcgatcttgc gaccgtacag ccccccttcg ctctcgccca cggcatcgct gaaccactgg
      121 aacagcacga acagccacca cagcagaccg gccagcagca gccagccgcc ccaggcggcg
      181 tcgttgatcc actggctggc accgatgatc atgagcagca ggccgaaggc ggccatcacc
      241 ggatgccgcg actcggcggg gacgtagtag tacggcgtct gaccgtgcgt ggtgctggac
      301 atcttcactt cactcctgtg ctctgaattc tatagctgct ggcgcttgtc tcacctgcgc
      361 tggacgttga ttggatggtt atcgatgggc ctcagccggg ggttgtgccg gacaccaccc
      421 agtggatgat gcccagcagc agcagcacga acagcacgac gccgatcagg cccgccagca
      481 tgatgtgaaa cggattgacc tgggccaggt cgcgctggta ttcgctgcgc ttgcgcacgc
      541 ccagaaagcc ccagcccacc atgcgcagcg tgcgccagaa ggccgggcca cccgccgcgg
      601 ctggcgcggc cacgtcgggc ggcgccggcg acaccggggt ctgcacggtg cgggccggct
      661 cgctcatgcg ccggctccgt gagggcgggc cagcgccgcg ctggactgcg gcggcgcggg
      721 cgtcttgcca cccacttcga aaaaggtgta ggagagcgtg atggtggtca cgtccttggg
      781 caggcgcgga tcgatcacga aggccaccgg ccattccttc ttctcgcccg gctccagcgt
      841 gtactgattg aagcagaagc attccagctt gttgaaatgc ggcgccgcct ggcgcggcgc
      901 gtagctgggg atggcctgcg ccgccatgcg ccgatcctgc acgttctgga actcgtacat
      961 cacggtggcc agctcgcccg ggtgcacctg cagggtgcgc tgcgcgggcc ggaattccca
     1021 cgggccgcgg gtgttggcgt cgaactcgac cgtgatgacg cggctgcgat cgacctgggt
     1081 gttgcgactg gccagccgcg ccgtctcgcc ggccgacccg ttgcccggca cctggcgctc
     1141 ggacagcgac aggatgttga tgccggtggc ttcgcagatg gcgcggtaga ccgggatcag
     1201 gctgtagccg aaggcgaaca tgccgcaggc caccacggcc agcttgccca gcatcttcag
     1261 gttttcggaa cgccggcgca tggccatccc cgcccgtcag ccgcccagca ccaccatgcg
     1321 cacgatgaag ccgacgaaga acgccagcgc caccgtgccc aggatcagcc ccagacggac
     1381 gttgcgcttg cgctgctgct ccgtggtcat ggccgacatc cgcgattcgt ccgccatcag
     1441 ccgatcacgc gggtggcgct ggcgtccagc ttgggcgggg tttcgtaggt atggaacggc
     1501 gccggcgagg gcacttccca ttccagaccc tcggcgcctt cccaggggcg ctgcggcgcc
     1561 ttggcgccct gcccccgcat caccggcagc accacgaaca ggaagaaata cagctgcgcg
     1621 aagccgaaga agaaggcgcc caccgaggcg atcaggttga agtcggcgaa ctgcatcggg
     1681 tagtcggcgt agcggcgcgg catgccggcc agacccagaa agtgcatcgg aaagaaggtg
     1741 acgttgaagg agatcatcga ccaccagaaa tggatcttgc cgcgcgtttc gttgtacatc
     1801 accccggtcc acttgggcga ccagtagtag aagccgccgt acagcgcgaa cagcgaaccg
     1861 gccaccagca cgtagtggaa gtgggcgatg atgtagtagg tgtcctggat ctgggtgtcc
     1921 accggcacca cggccgggat cacgccggtg aagccgccga tggtgaacac gaagatgaag
     1981 cccacggccc acagcatcgg ggtctcgaag gtcatcgagc cgcgccacat ggtggccacc
     2041 cagttgaaga tcttcacccc ggtgggaatg gcaatcagca tggtgccgta catgaagaac
     2101 agctggcccg tgaccggcat gccggtggtg aacatgtggt g
//
LOCUS       DAOOOG010000313         2108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_66062_length_2108_cov_27.584998, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000313 DAOOOG010000000
VERSION     DAOOOG010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66062_length_2108_cov_27.584998"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PK242_13845"
     CDS             <1..488
                     /locus_tag="PK242_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOZ95149.1"
                     /translation="GDDYTLQPDPQELVEAARQLTRDGFTVFPYCTEDLVTARRLLDA
                     GCPLLMPWGAPIGSGQGLVNRFALRLLRERLPDVVLIIDAGLGAPSHAAQALEMGFDA
                     VLLNSAVSQSPDPVRMAGAFRLAIEAGRAAYRAGVMARQDFAVATTPVTGNPFLIGNA
                     P"
     gene            485..>2108
                     /locus_tag="PK242_13850"
     CDS             485..>2108
                     /locus_tag="PK242_13850"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOZ95150.1"
                     /translation="MTPMGQAPAATKAVAQPIVWSVAGNDSGGGAGLAADLRAAAAFG
                     VHLCPVVAAITAQHSQAVTRVEPVAADLLDAQLAALAADLPPAAIKTGLLGSADNVAV
                     LARWVDRLRQRAPLALVVDPVLGASSGAAFADEAVLRAYRELLLPRATLVTPNEHEAR
                     RLLGGAPPNPRPSGQRAGVVGQPGEPSDVPALAVALRAQGAQAVAITGGDRVHAPGWA
                     LDWIDTEHARGWLALPRLESRHTHGTGCTFASSAAAALALGFVAADALVLAKMATAHA
                     IAHGHAAGQGAGPVAARPGFAVEPARMPLLSCSELDSCWRFLGARQSPIPLETQAPGL
                     YAIVDSAERVRQVVAAGARTVQLRIKTPAAPPAAWQDELRCAIRQALGACREAGATLV
                     VNDHWRLAAELTAGAARGIAVHLGQEDLLALGDAGRAELQACGLPLGISSHSLWELAR
                     ARSLAPWYVACGPVWPTLTKAMPWRAQGLDNLSWWVHMAGVPVVAIGGILEPAQATQA
                     TRSGAAGVCVVRGLGADPAATLPVWQAAIAAGR"
BASE COUNT          246 a          792 c          800 g          270 t
ORIGIN      
        1 tcggcgacga ctacaccctg cagcccgacc cgcaggagct ggtcgaggcg gcgcgccagc
       61 tcacgcgcga cggcttcacg gtgttccctt actgcaccga ggacctggtg accgcccggc
      121 gcctgctgga cgccggctgc ccgctgctga tgccctgggg cgcgccgatc ggatcgggcc
      181 agggcctggt caaccggttc gcgctgcgcc tgctgcgtga gcgcctgccg gacgtggtgc
      241 tgatcatcga cgccggcctg ggcgcgccca gccacgccgc gcaggcgctg gagatggggt
      301 ttgacgccgt gctgctcaac agcgccgtca gccagtcgcc cgatccggtg cgcatggccg
      361 gcgccttccg cctggcgatc gaggccgggc gcgccgccta tcgtgccggg gtaatggcgc
      421 ggcaggattt cgcggtcgcc accacgcccg tcaccggcaa cccgtttctg atcggaaacg
      481 cgccatgacg cccatgggac aagcgccagc agctacaaaa gcagtagctc aaccgatcgt
      541 ctggagtgtc gccggcaacg actcgggcgg cggcgcgggc ctcgccgccg acctgcgcgc
      601 ggcggcggcg ttcggcgtgc atctgtgccc ggtggtggcg gccatcacgg cgcagcactc
      661 gcaggccgtg acgcgcgtcg agccggtggc ggccgacctg ctcgacgcgc aactggcggc
      721 actggcggcg gacctgccgc cggccgccat caagaccggg ttgctgggca gcgccgacaa
      781 cgtggccgtg ctggcgcgct gggtcgaccg gctgcgccag cgggcgccgc tggcgctggt
      841 ggtcgatccg gtgctggggg ccagcagcgg cgccgccttt gccgacgagg ccgtgctgcg
      901 cgcctaccgc gagctgctgc tgccgcgcgc cacgctggtc acgcccaacg agcacgaggc
      961 gcggcggctg ctgggtggcg caccgccgaa cccgcgcccc tcggggcaga gggctggggt
     1021 ggtgggccag cccggcgagc cgagcgacgt gccggcgctg gccgtggccc tgcgcgcgca
     1081 gggcgcgcag gcggtcgcca tcaccggcgg cgaccgcgtg cacgcgccgg gctgggcgct
     1141 ggactggatc gataccgagc acgcccgtgg ctggctggcg ctgccgcgcc tcgaatcccg
     1201 gcacacgcac ggcaccggct gcacctttgc cagcagcgcc gcggcggcgc tggcgctggg
     1261 cttcgtggcg gccgacgcgc tggtgctggc caagatggcc accgcccacg ccatcgccca
     1321 cggtcacgcg gccgggcagg gcgccgggcc ggtggcggcg cgccccggct ttgccgtcga
     1381 gccggcgcgc atgccgctgc tgtcatgctc cgaattggat agctgctggc gctttctggg
     1441 tgcgcgccag agcccgattc cgcttgaaac ccaggcgcct ggcctgtacg ccatcgtcga
     1501 cagcgccgag cgcgtgcgcc aggtcgttgc cgcgggcgcg cgcacggtgc agttgcgcat
     1561 caagacgccc gctgcaccgc ccgccgcctg gcaggacgaa ttgcgctgcg ccatccggca
     1621 ggcgctcggc gcctgccgcg aggccggcgc caccctggtc gtcaacgacc actggcggct
     1681 cgccgccgaa ctgacggccg gcgcggcgcg cggcatcgcc gtgcacctgg gccaggaaga
     1741 cctgctggcc ctgggcgacg ccggccgcgc cgagctgcaa gcctgcggcc tgccgctggg
     1801 catcagcagc cacagcctgt gggagctggc gcgcgcgcgc agcctggcgc catggtacgt
     1861 cgcctgcggc ccggtctggc cgaccctgac caaggccatg ccctggcgcg cccagggcct
     1921 ggacaacctg agctggtggg tgcacatggc cggtgtgccg gtggtggcga tcggcggcat
     1981 cctggagccc gcccaggcca cgcaggcgac gcgaagcggc gccgccggcg tgtgcgtggt
     2041 gcgtggcctg ggcgccgacc cggccgccac cctgcccgtg tggcaggccg ccatcgccgc
     2101 cggccgcg
//
LOCUS       DAOOOG010000314         2101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_66374_length_2101_cov_23.080156, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000314 DAOOOG010000000
VERSION     DAOOOG010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66374_length_2101_cov_23.080156"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PK242_13855"
     CDS             complement(<1..607)
                     /locus_tag="PK242_13855"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOZ95151.1"
                     /translation="MTRRAALALGAGAFGAPGVLAQVPSGWPGLPARIATQRPDVQSV
                     AVVQRGALVFDFLRAGLRADSLQDVQSVTKSVLALLFGCALADGHVRGVDELVALRLP
                     QLLRQGGDERLRRLRFVHLLTMTAGWPGELTARRDRDDDLRWLALRPFVAEPGTRFVY
                     DNGAANLLALALANAVGRPLADYARDRLLAPLGISRFAWRRG"
     gene            complement(684..983)
                     /locus_tag="PK242_13860"
     CDS             complement(684..983)
                     /locus_tag="PK242_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOZ95152.1"
                     /translation="MHLLRLSIHATPGARRTEAAGAHGQALRVRLAAPPVDGKANAAL
                     IAWAAAALGVPRAQVELLHGASGRQKVLELRFDDAAALAAAQARVACWMAGGADA"
     gene            complement(1002..>2101)
                     /locus_tag="PK242_13865"
     CDS             complement(1002..>2101)
                     /locus_tag="PK242_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl/propionyl-CoA carboxylase subunit alpha"
                     /protein_id="HOZ95153.1"
                     /translation="GFAQADVKREGWAMECRINAEDPFRNFLPSTGRLVRFAPPATDL
                     QQGQATIAHGVRVDTGVVDGGEIPMFYDSMIAKLIVHGKDRADAIARMREALNGFVIK
                     GVQSNIPFQAALLAHPKFVAGDFNTGFIAEHYAHGFSAADVPHADPSFLVALAARLHM
                     FYRNRAQGLQGQFWEGFKTLPKRDFTVCVLGKEGHNEYVDVSLSEVVRGEPTQVTVHA
                     DGGDRVYELALDNYLGDTRIAGRCNGRPFTAQMERGQTRNPLAIRVMHDGTQRDAIVM
                     HPGTAVLHKLMPYKAPPDMSKFVLSPMPGLLVQVAVQPGQEVKAGERVAVIEAMKMEN
                     VLLASTDGVVKEVKARQGDSLAVDQAIVEFE"
BASE COUNT          316 a          763 c          724 g          298 t
ORIGIN      
        1 cgccccggcg ccaggcaaag cgggagatgc ccagcggcgc cagcagccga tcgcgcgcgt
       61 agtcggccag cggacgaccg accgcgttgg ccagcgccag cgccagcagg ttggcggcgc
      121 cgttgtcgta gacaaaacgc gttcccggct cggccacgaa gggccgcagc gccagccagc
      181 gcaggtcgtc gtcacggtcg cgccgtgccg tcagctcgcc gggccagccg gccgtcatgg
      241 tgagcaggtg gacaaagcgc aggcgccgca ggcgctcgtc gccgccctgc cgcagcagct
      301 gcggcaggcg cagcgccacc agctcgtcga caccgcgcac atgcccatcg gccagcgcgc
      361 agccgaacag cagcgccagc acgctcttgg tgaccgactg cacgtcctgc aggctgtcgg
      421 cgcgcagccc ggcgcgcaag aagtcgaaca ccagggcgcc gcgctgcacc acggccacgc
      481 tctgcacgtc gggccgctgc gtggcgatgc gcgccggcag acccggccag ccggacggca
      541 cctgcgccag caccccgggg gcgccgaagg caccggcgcc cagagccagc gcggcacgcc
      601 gggtcaagcg gcattcaggg caaaaaccgg cttcagcgat tacgggataa gcgcgagcag
      661 ctattgtttt tgaagttcca gactcacgca tcggcgccgc ccgccatcca gcacgccacc
      721 cgcgcctggg cggcggccag cgccgccgca tcgtcaaagc gcagctccag caccttctgc
      781 cgcccgctgg cgccgtgcag caactcgacc tgcgcgcgcg gcacgcccag cgccgccgcg
      841 gcccaggcga tcagcgccgc attggccttg ccgtcgaccg gcggcgccgc cagccgcacg
      901 cgcagggcct ggccatgggc gcccgctgct tcggtgcggc gcgcgccggg cgtggcatgg
      961 atcgacagcc tgagcaggtg cacggcgagc ctgcggccgg gtcattcgaa ctcgacgatg
     1021 gcctgatcga ccgccaggct gtcgccctgc ctggccttga cctccttgac caccccgtcg
     1081 gtgctggcca gcagcacgtt ttccatcttc atggcctcga tcacggccac gcgttcgccc
     1141 gccttgactt cctggcccgg ctgcacggcc acctgcacca gcaggcccgg catgggcgac
     1201 aggacgaatt tgctcatgtc gggcggcgcc ttgtagggca tcagcttgtg cagcacggcc
     1261 gtgcccgggt gcatgacgat ggcgtcccgc tgcgtgccgt catgcatcac gcggatcgcc
     1321 agcgggttgc gcgtctgccc gcgctccatc tgggccgtga agggccgacc gttgcagcgc
     1381 ccggcgatgc gggtgtcacc caggtagttg tccagcgcca gttcatagac gcggtcgccg
     1441 ccgtccgcat gcacggtgac ctgtgtaggc tcgccgcgca ccacctcgct caggctgacg
     1501 tcgacatact cgttgtgccc ttccttgccg agcacgcaga cggtgaagtc gcgcttgggc
     1561 aaggtcttga agccctccca gaactgcccc tgcaggccct gggcgcggtt gcggtagaac
     1621 atgtgcaggc gcgcggccag cgccaccagg aagcttgggt cggcgtgcgg cacgtcggcg
     1681 gcggagaagc cgtgcgcgta atgctcggcg atgaagccgg tgttgaagtc gccggccacg
     1741 aacttcgggt gcgccagcag cgcggcctgg aacgggatgt tgctctgcac gcccttgatg
     1801 acgaaaccat tgagcgcctc gcgcatgcgg gcgatggcgt ccgcgcggtc cttgccgtgc
     1861 acgatcagct tggcgatcat cgagtcgtag aacatcggga tctcgccgcc gtcgacgacg
     1921 ccggtgtcga cacgtacgcc atgcgcgatc gtggcctggc cctgctgcag gtccgtcgcg
     1981 ggcggcgcaa agcgcaccag gcggccggtg gagggcagga agttgcggaa cgggtcttcg
     2041 gcgttgatgc ggcactccat ggcccagcct tcgcgcttca cgtcggcttg cgcaaagccc
     2101 a
//
LOCUS       DAOOOG010000315         2100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_66413_length_2100_cov_25.869927, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000315 DAOOOG010000000
VERSION     DAOOOG010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66413_length_2100_cov_25.869927"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PK242_13870"
     CDS             <1..878
                     /locus_tag="PK242_13870"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOZ95154.1"
                     /translation="HQGQGVAKFVELVKAKSNGEIDIKPYYNAALGDDVKVTSALQGG
                     TIEFTVPVTTTLTGMVKAYSIFDFPFLFKNPEEADKVLDGPVGQKLLETLPAHGLVGL
                     AYWENGFFNATNSKHPITRVEDFAGLKFRSIQAKITQETIRALGANPVPLAIPELYTA
                     LETRTIDGQGTPNAVIAALKLNEVQKFLSITRHTYSPFIPLVSKAFWDKLTPAQQKIL
                     KDAAIEARTYQRQVARQQSDAAQKAMAERGMKVNEVSDAERARMRERVQPVWNMFTPD
                     VGQDLVGQVEAQLKK"
     gene            complement(936..1811)
                     /locus_tag="PK242_13875"
     CDS             complement(936..1811)
                     /locus_tag="PK242_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ95155.1"
                     /translation="MPHSSLYLRCAGREIHVLDWTPAGGAVRGTVVAWHGLARTGRDM
                     DELAAHLSARGWRVVCPDTVGRGLSQWAEQPEHEYCLAFYARLARELLAQLGIERCHW
                     VGTSMGGAIGTVCASGLFEPALAPRIQSLVLNDNAPELAQPAIERIRAYAGQPPAFAT
                     VTELEGFFRAAYQPYGFLTDAQWRRLTETSTRRLPDGRVTPHYDPAIVGQFTHHPDDY
                     LIWHHYDALTLPVLLLRGEDSDLVLRPTAEQMRLRGPGARGRLQWVEVPGCGHAPALN
                     VPEQLALVSDFIERA"
     gene            complement(1823..>2100)
                     /locus_tag="PK242_13880"
     CDS             complement(1823..>2100)
                     /locus_tag="PK242_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HOZ95156.1"
                     /translation="GFIGLPAVLPGYDSRYVPLHLYLNRALDAVYAHLREGKALPPSQ
                     VVRTTPRGGTPGSAPALTAAHVPALAASPAAGNAIGFAAGHTIVIPD"
BASE COUNT          341 a          711 c          762 g          286 t
ORIGIN      
        1 cccaccaggg ccagggcgtg gccaagttcg tcgaactggt caaggccaag agcaacggcg
       61 agatcgacat caagccctac tacaacgccg ctctgggcga cgacgtcaag gtcacatcgg
      121 cgctgcaggg tggcacgatc gagttcaccg tgccggtgac caccacgctg accggcatgg
      181 tcaaggcgta ctcgatcttc gacttcccgt tcctgttcaa gaaccccgag gaggccgaca
      241 aggtgctcga cggcccggtc ggccagaagc tgctggagac gctgccggcg cacggcctgg
      301 tgggtctggc ttattgggag aacggctttt tcaacgccac caacagcaag cacccgatca
      361 ccagggtgga agacttcgcg ggcctgaagt tccgctccat ccaggccaag atcacccagg
      421 aaaccatccg ggcgctgggc gccaacccgg tgccgctggc gatccccgag ctctacaccg
      481 cgctcgagac ccgcaccatc gacggtcagg gcacgcccaa cgcggtgatc gccgcactca
      541 agctcaacga ggtgcagaag ttcctctcca tcacgcgcca tacctacagc ccgttcatcc
      601 cgctggtgtc caaggccttc tgggacaagc tgacgccggc gcagcagaaa attctgaaag
      661 acgccgccat cgaggcgcgc acctaccagc gccaggtggc gcgccagcag tccgacgccg
      721 cccagaaagc catggccgag cgcggcatga aggtcaacga ggtgtcggac gccgagcgcg
      781 cccgcatgcg cgagcgcgtg cagcctgtgt ggaacatgtt cacgcccgac gtcggacagg
      841 acctggtggg ccaggtcgag gcgcagctca agaagtaagg gcgcgggggc cgcgggcgcg
      901 gtctgcccgc ggcatcaggc ggcgccgggc tggcctcagg cgcgctcgat gaaatcgctc
      961 accagcgcca gttgctcggg cacgttgagc gctggcgcgt ggccgcagcc gggtacttcc
     1021 acccactgca ggcgaccgcg ggcgccgggg ccgcgcagcc gcatctgctc ggcggtcggg
     1081 cgcagcacca ggtccgaatc ctccccgcgc agcagcagca ccggcagggt gagtgcgtcg
     1141 tagtggtgcc agatcaggta gtcgtcgggg tggtgcgtga actggccgac gatggccggg
     1201 tcgtagtgcg gcgtcacgcg gccgtcgggc agccggcgag tgctggtttc ggtcaggcgg
     1261 cgccactggg cgtcggtgag aaacccgtac ggctggtagg cggcgcggaa gaacccttcc
     1321 agctcggtca cggtggcgaa cgccggcggt tggccggcgt aggcccggat gcgctcgatg
     1381 gccggctgcg ccagttcggg cgcgttgtcg ttcagcacca ggctctggat gcggggtgcc
     1441 agcgcgggct cgaacagtcc cgaggcgcag acggtgccga tggcgccgcc catcgaggtg
     1501 ccaacccagt ggcagcgctc gatgccgagc tgcgccagca gctcgcgcgc cagccgggcg
     1561 tagaaggcca ggcagtactc gtgctcgggt tgctcggccc actggctcag cccgcgcccc
     1621 acggtgtcgg ggcagaccac gcgccagccg cgcgccgaca gatgggccgc cagctcgtcc
     1681 atgtcgcgcc cggtgcgcgc cagaccgtgc caggcgacca ccgtgccgcg cacggcgccg
     1741 ccggcgggcg tccagtcgag cacatggatt tcacgcccgg cgcagcgcag gtacagggac
     1801 gagtggggca tggcgttcga cttcagtcgg ggatgacgat ggtgtggccg gccgcgaagc
     1861 cgatggcgtt gccggcggcg gggctggcgg ccagcgctgg cacgtgggcc gccgtcaagg
     1921 ccggcgcgct gcccggcgtg ccgccacgcg gcgtggtgcg cactacctgg ctgggcggca
     1981 gcgccttgcc ttcgcgcagg tgggcgtaca ccgcgtccag ggcgcggttc aggtacaggt
     2041 gcagcggcac gtagcggctg tcgtagccgg gcagcacggc gggcaggccg atgaagccgt
//
LOCUS       DAOOOG010000316         2098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_66512_length_2098_cov_22.727362, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000316 DAOOOG010000000
VERSION     DAOOOG010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66512_length_2098_cov_22.727362"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..903
                     /locus_tag="PK242_13885"
     CDS             34..903
                     /locus_tag="PK242_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOZ95157.1"
                     /translation="MSEVTPPAHRPEDSTDTPRRQRSIQSIEVGMRVLDVLTEAGQAL
                     PLKEVAQRAAMPPGKAHPYLVSFMNVGLVKQHPLTGLYELGPKALQLGLAALEQLDPL
                     TEASGEAAQLAADSGLSVALVVRGNLGPTVVRLDEPRYPLHMNLRVGTVMSMLGTVTG
                     RVFAAFLPDAMVRAMVAGEQERVIGGRSGAGVARPQGGLGWLDSPEIQRTLADIRTLG
                     VGSGVGLPLPGVNTLSAPVFDGDGTMVLAISVIGPHGVFDPDPHGPIAALLRASAARV
                     SERLGARAGHSRA"
     gene            1164..>2098
                     /locus_tag="PK242_13890"
     CDS             1164..>2098
                     /locus_tag="PK242_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ95158.1"
                     /translation="MSEKKFASHADVEEKQISWDKLSDNAYAYTAEGDPNTGVIIGDD
                     GVMIIDATATPVAAQGVIRKIREITDKPIKYVVLTHYHAVRVLGASGYKNEGLEQIIA
                     SQDTWDLIVERGQQDMDSEIGRFPRLFQAVESVPGLTWPTMTFTGQMTVWLGKLEVKI
                     QQIGRGHTKGDTIVYLPSQNICFSGDLVEADAACYSGDAYLSEWPQTLERLAAMKFDK
                     LVPGRGPTLMTSEAVQKGLAYTRDYVGTLYQAAQQAVAQGKDLKATMAHARQLMDPKF
                     GHVFIYEHCLPFNVTRAHDEASGIRDPRIWTAERDR"
BASE COUNT          355 a          744 c          715 g          284 t
ORIGIN      
        1 cggcgccgca tgcgcgatcc ggataatggc gccatgtccg aagtcacgcc ccccgcccac
       61 cggcccgaag acagcaccga cacgccgcgg cgccagcgca gcatccagtc gatcgaggtc
      121 ggcatgcgcg tgctggacgt gctgaccgag gccggccagg ccctgccgct caaggaagtg
      181 gcgcagcgtg ccgccatgcc gccgggcaag gcgcacccgt acctggtcag cttcatgaac
      241 gtcgggctgg tcaagcagca cccgctgacc ggcctgtacg agctggggcc gaaggcgctg
      301 cagctcggcc tggcggcgct ggagcagctc gacccgctga ccgaagccag cggcgaagcg
      361 gcgcagctgg ccgccgactc cggcctgtcg gtggcgctgg tggtgcgcgg caacctggga
      421 cccacggtgg tgcggctgga cgagccgcgc tacccgctgc acatgaacct gcgcgtgggc
      481 accgtgatgt ccatgctggg caccgtcacc gggcgcgtgt ttgccgcctt tctgcccgac
      541 gcgatggtgc gcgccatggt ggcgggcgaa caggaacgcg tgatcggcgg ccgcagcggc
      601 gccggtgtgg cgcggccgca gggcgggctg ggctggctgg acagcccgga gatccagcgc
      661 acgctggccg acatccgcac gctcggcgtc ggcagcggcg tggggctgcc gctgccgggc
      721 gtcaacaccc tgagcgcacc ggtgttcgac ggcgacggca ccatggtgct ggcgatttcg
      781 gtgatcgggc cgcatggcgt gttcgacccc gatccgcacg gccccattgc ggccctgttg
      841 cgcgcgtcgg ccgcgcgcgt gtcggagcgc ctgggcgcgc gcgccgggca cagccgcgcc
      901 tgaggcggca ccggccttgg ccgcggcggc gccccggccc tgcgtcagga ccgcttcgcg
      961 ccccggcagc cggggtggcg agcctgcatc agccttgcac agacccgtcc atcaggccgg
     1021 gtcgagccgg ccgcgcgcgc ctggcccgac gccgcgccgg tgcgcccgcc aacaccggtt
     1081 gtcttgcgga caaattagat attgataaat ccattatctt tagctaaaat cgcgccatcg
     1141 tttcacagac cggagaccac cccatgtccg agaagaaatt cgcctcccac gccgacgtcg
     1201 aagaaaagca gatcagttgg gacaagctga gcgacaacgc ctacgcctac accgccgaag
     1261 gcgaccccaa caccggcgtc atcatcggcg acgacggcgt gatgatcatc gacgccaccg
     1321 ccacccccgt ggcggcgcag ggcgtcatca ggaagatccg cgagatcacc gacaagccca
     1381 tcaagtacgt ggtgctgacg cactaccacg cggtgcgcgt gctgggcgcg tcgggctaca
     1441 agaacgaagg gctggagcag atcatcgcca gccaggacac ctgggacctg atcgtcgagc
     1501 gcggccagca ggacatggat tccgagatcg ggcgctttcc gcgcctgttc caggcggtgg
     1561 aaagcgtgcc cggcctgacc tggccgacca tgaccttcac cggccagatg accgtgtggc
     1621 tgggcaagct ggaagtcaag atccagcaga tcggccgcgg ccacaccaag ggcgacacca
     1681 tcgtctacct gccgagccag aacatctgct tctcgggcga tctggtcgaa gccgacgccg
     1741 cctgctactc gggtgacgcc tacctgtcgg agtggccaca gacgctggag cggctggccg
     1801 ccatgaagtt cgacaagctg gtgcccggcc gtggccccac cctgatgacg tccgaggcgg
     1861 tgcagaaagg cctggcctac acgcgcgact acgtcggcac cctgtaccag gcggcgcaac
     1921 aggccgtggc ccagggcaag gacctgaagg ccaccatggc gcacgcgcgc cagctgatgg
     1981 acccgaagtt cggccacgtc ttcatctacg agcactgcct gccgttcaac gtcacgcgcg
     2041 cccacgacga agccagcggc atccgcgatc cgcgcatctg gaccgccgag cgcgaccg
//
LOCUS       DAOOOG010000317         2097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_66564_length_2097_cov_26.908423, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000317 DAOOOG010000000
VERSION     DAOOOG010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66564_length_2097_cov_26.908423"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="PK242_13895"
     CDS             <1..173
                     /locus_tag="PK242_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOZ95159.1"
                     /translation="SARLADEDLSINFADCTTPSDRVAVIATQPLTCDERWTAFEPGS
                     LKVFVDGALQNL"
     gene            complement(248..1888)
                     /locus_tag="PK242_13900"
     CDS             complement(248..1888)
                     /locus_tag="PK242_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ95160.1"
                     /translation="MPDTDPPAAADRAPPADDLGALAPFRHRVFSLLWSTWLIANLCM
                     WMNDVAAAWLMTTLTDRPNWVALVQTAATLPVFLLGLPSGALADILNRKHFLLFTQLW
                     VALVGALLALVAFAGVITPPLLLLLLFANGVGLALRWPVFAAIVPELVPRAQLPAALA
                     LNGASMNAARILGPLAAGALIASLGSAWVFGLNAVLSVAAAIVISRWRRPHRPHPLGR
                     ESLRGAMRVGLQYVMQSYHLKGVLLRVALFFFHSTALIALLPLVARQIEGGHAGTFTV
                     LLAAMGAGAIGSTFVLPRWRQMFTRDRLVLAGACTQALMMALMAFTHSLWLAVPAMLL
                     CGVAWLTTANSLSVSAQISLPDWVRARGMSMYQMAIMGATAVGAAVWGQVATWSSVPV
                     TLGIGAASGIASMALATWRWPDRGSIDDPTPAGPWPLPQAEAPPPHGHVIVTVEYQID
                     PAQAPAFRALMEESRRSRLRVGAVGWELLSDINDPGRFIEVIEDASWTEHLRRFERVS
                     VQDAALRERKLAFHIGGQPPIVRRAVRESTVRGERPST"
BASE COUNT          335 a          735 c          772 g          255 t
ORIGIN      
        1 ccagcgcccg gctggccgac gaggacctga gcatcaactt cgccgactgc accacaccga
       61 gcgaccgcgt ggccgtgatc gccacccagc cgctgacctg cgacgagcgc tggacggcct
      121 tcgagccggg cagcctgaag gtgttcgtgg acggggcatt gcagaatctg tgagcccggt
      181 tgggctccag aaagctatta aaacgatagc tgctggcgca ggtcgttgct gcgccagagc
      241 tgaatttcta tgttgatggg cgctcgccgc gcaccgtgct ttcgcgcact gcgcgccgga
      301 cgatgggcgg ctgcccgccg atgtgaaagg ccagcttgcg ctcgcgcagc gcggcgtcct
      361 gcacgctgac gcgctcgaaa cggcgcaggt gctcggtcca ggaggcgtcc tcgatcacct
      421 cgatgaagcg gcccgggtcg ttgatatccg acagcagctc ccagcccacc gcgcccacgc
      481 gcaggcgcga gcggcggctt tcttccatca gggcgcggaa ggccggcgcc tgggccgggt
      541 cgatctggta ttcgacggtg acgatcacgt ggccatgggg cggcggcgcc tcggcctgcg
      601 gcaggggcca ggggccggcc ggcgtggggt cgtcgatgct gccgcggtcg ggccagcgcc
      661 aggtcgccag cgccatgctg gcgatgccgc tggcggcacc gatgcccagc gtgacgggca
      721 cgctgctcca ggtcgccacc tggccccaca cggcggcgcc gacggcggtg gcgcccatga
      781 tggccatctg gtacatcgac atgccgcgcg cgcgcaccca gtcgggcagg ctgatctgcg
      841 ccgacacgct gagcgagttg gcggtggtca gccaggccac gccgcacagc agcatggccg
      901 gcaccgccag ccacaggctg tgggtgaacg ccatcagcgc catcatcagc gcctgcgtgc
      961 aggcgccggc cagcaccagc cggtcgcgcg tgaacatctg gcgccaccgc ggcagcacga
     1021 aggtcgagcc gatggcgccg gcgcccatgg cggccagcag cacggtgaag gtgccggcgt
     1081 ggccgccctc gatctggcgc gccaccagcg gcagcagcgc gatcagcgct gtggagtgaa
     1141 agaagaacag cgccacgcgc agcagcaccc ccttgaggtg gtaggactgc atcacatact
     1201 gcaggcccac gcgcatggca ccgcgcaggc tctcgcgccc cagcgggtgc ggccggtgcg
     1261 ggcgccgcca gcgcgaaatc acgatcgctg ccgccaccga cagcacggcg ttgagcccga
     1321 acacccaggc gctgcccagg ctggcgatca gggcgccggc cgccagcgga cccaggatgc
     1381 gggcggcgtt catcgacgcg ccattgagtg ccagcgccgc cggcagctgg gcgcgcggca
     1441 ccagctcggg cacgatggcg gcaaacaccg gccagcgcag cgccagcccc acgccgttgg
     1501 caaacagcag cagcagcagc agcggcggcg tgatcacgcc ggcaaaggcc accagcgcca
     1561 gcagcgcccc caccagcgcc acccacagct gggtgaacag caggaagtgc ttgcggttga
     1621 ggatgtcggc cagcgcaccc gaaggcaggc cgagcagaaa caccggcagc gtggcggcgg
     1681 tctgcaccag cgccacccag ttgggccggt cggtcagcgt ggtcatcagc caggcggcgg
     1741 ccacgtcgtt catccacatg cacaggttgg cgatcagcca ggtgctccac agcagggaga
     1801 acacacggtg gcgaaacggc gccagcgcgc caaggtcgtc ggccggcggg gcgcggtcgg
     1861 cggcggccgg ggggtcggtg tcaggcatgg gcggtggccg atccggccgt cggccgccgg
     1921 gcgcctgcgc gccaggtggg cgccgcaggc gcgggggggc gggcgggctc cctggccctg
     1981 atcggcgggt cgatcacgct atcgggggcg tccggccacc acgttttcga ccgcgtccag
     2041 aaacagcgcc ggcacgtcgc agccggtctg gtcgaggatt tcgcgaaagc cggtggg
//
LOCUS       DAOOOG010000318         2088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_66993_length_2088_cov_26.626660, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000318 DAOOOG010000000
VERSION     DAOOOG010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66993_length_2088_cov_26.626660"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /gene="rpoB"
                     /locus_tag="PK242_13905"
     CDS             complement(<1..1381)
                     /gene="rpoB"
                     /locus_tag="PK242_13905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797537.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOZ95161.1"
                     /translation="MAYTFTERKRIRKDFGTRESVLQVPYLLQMQKDAYTAFLQADTP
                     PKKRSREGLQAAFESAFPIVSHNGFVEMKFIEYNLAKPAFDVRECQTRGLTFSSAVRA
                     RVQLIIYDRESSTPQSKVVKEVKEQEVYMGEVPLMTDKGSFIVNGTERVIVSQLHRSP
                     GVFFEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDILFFRVDRRRKMPVTILLK
                     AIGLNPEQILAHFFVNDHFRLMDSGAQLEFVADRLKGEVARFDITDKSGKVVVAKDKR
                     ITARHTRELEQSGTTHISVPEDFLVGRVVARNVVDADTGEIIAKANDELTEALLKKLR
                     DAGVQELPCIFTNELDQGAYISQTLRMDETADELAARVAIYRMMRPGEPPTEDAVQAL
                     FQRLFYNPDTYDLSRVGRMKFNAKVGREESTGPMVLANEDILAVVKILVELRNGRGEV
                     DDIDHLGNRR"
     gene            complement(1742..>2088)
                     /gene="rplL"
                     /locus_tag="PK242_13910"
     CDS             complement(1742..>2088)
                     /gene="rplL"
                     /locus_tag="PK242_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOZ95162.1"
                     /translation="DSMTVMELNDLVKAIEEKFGVSAAAMAAPAAGGAAGGAAAAAEE
                     KTEFDVVLAEAGANKVSVIKAVREITGLGLKEAKDLVDGAPKTVKEGMAKADAEAAKK
                     KLEEAGAKVELK"
BASE COUNT          336 a          658 c          693 g          401 t
ORIGIN      
        1 cgcggcggtt gcccaggtgg tcgatgtcgt ccacctcgcc acggccgttg cgcagctcga
       61 ccaggatctt gacgacggcc aggatgtcct cgttggccag caccatgggg ccggtggatt
      121 cctcgcgacc gaccttggcg ttgaacttca tgcgaccgac acgcgacaga tcgtaggtgt
      181 cggggttgta gaaaaggcgc tggaacaagg cctgcaccgc gtcctcggtg ggcggctcgc
      241 ccgggcgcat catgcggtag atggccacgc gcgcggccaa ctcgtcggcc gtttcgtcca
      301 tgcgcagcgt ctgcgagatg taggcgcctt ggtccagctc gttggtgaag atgcacggca
      361 gctcctgcac gccggcatcg cgcagcttct tgagcagcgc ctcggtcagc tcgtcgttgg
      421 ccttggcgat gatttcaccg gtgtcggcat cgacgacgtt gcgggccacc acgcggccca
      481 ccaggaagtc ctcgggcacg ctgatgtgcg tggtgcccga ttgctccagc tcgcgcgtgt
      541 ggcgcgcggt gatgcgcttg tccttggcca ccaccacctt gcccgacttg tcggtgatat
      601 cgaaacgcgc cacctcaccc ttcagacggt cggcgacgaa ctcgagctgc gcgccgctgt
      661 ccatcaggcg gaagtggtcg ttgacgaaga agtgggccag gatctgctcg ggattcaggc
      721 cgatggcctt gagcaggatg gtgaccggca tcttgcggcg gcggtcgacg cggaagaaca
      781 ggatgtcctt ggggtcgaac tcgaagtcca gccaggagcc gcggtaggga atgatgcgcg
      841 ccgaaaacag cagcttgccc gagctgtgcg tcttgccctt gtcgtgctcg aaaaacacgc
      901 caggcgagcg gtgcagctga gacacgatca cgcgctcggt gccgttgacg atgaacgagc
      961 ccttgtcggt catcaggggc acctcgccca tgtagacctc ctgctccttc acttccttga
     1021 ccaccttgga ctgcggggtg gaggactcgc ggtcgtagat gatcagctgc acgcgcgcgc
     1081 gcacggcgga agagaacgtc aggccacgcg tctgacactc gcgcacgtcg aacgccggct
     1141 tggccaggtt gtattcgatg aacttcatct ccacaaagcc gttgtgcgag acgatcggga
     1201 aggcggactc gaacgcggcc tgcaaaccct cgcgactgcg cttcttgggc ggcgtgtcgg
     1261 cttgcaggaa tgccgtgtag gcgtctttct gcatctgcag caggtagggc acttgcagca
     1321 cgctttcacg ggtgccgaag tccttgcgga tgcgcttgcg ttcagtgaag gtataggcca
     1381 tgaaatctcc gggctggatg cgtgagggtc ctggacatct ggcgacttgg catcaggcgc
     1441 tgggcctgaa aagcttggtg gctggccact accagccgat ggcggacggc cgcgcgttgc
     1501 acgcggcccg aaccaagtgg cctcctgcag tcgggttcag aagacacgtg aaagcggctt
     1561 gcctgaagcc actttcacgt gcgctctggg agcgccgtga aaggctggtc gtaccaccag
     1621 cccgcacatc gcgactggag cgtggcgagc ggcgcgaggt cggctcgaga agcgccgccg
     1681 tatttgagta cggcgagcat cggagggccg agatcgcgcc gcgcagcagc tccagatcgg
     1741 gttacttgag ctcgaccttg gcgccggctt cttccagctt cttcttggcc gcttcggcgt
     1801 cggccttggc catgccttcc ttgaccgtct tgggcgcgcc gtcgaccagg tccttggctt
     1861 ccttcaggcc caggccggtg atctcgcgca ccgccttgat gaccgacacc ttgttggcac
     1921 cggcttcggc cagcacgacg tcgaattcgg tcttctcttc ggccgcggcg gccgcgccac
     1981 cggcggcgcc accagcagcc ggagcggcca tggcggcggc gctcacgccg aacttctctt
     2041 cgatggcttt caccaggtcg ttgagctcca tgaccgtcat gctgtcga
//
LOCUS       DAOOOG010000319         2084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_67157_length_2084_cov_36.742730, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000319 DAOOOG010000000
VERSION     DAOOOG010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67157_length_2084_cov_36.742730"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2084
                     /locus_tag="PK242_13915"
     CDS             <1..>2084
                     /locus_tag="PK242_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOZ95163.1"
                     /translation="SVIEAGLKCLQGKGIVNSISMKEGVAEFKRQATLIRRYGAAAVV
                     MAFDEQGQADTFARKIEICERAYRVLVDEVGFAPEDIIFDPNIFAIATGIEEHANYAV
                     DFIEAVRWIKQHLPGAKVSGGVSNVSFSFRGNDPVREAIHTVFLYHAIQAGMDMGIVN
                     AGMVGVYDDLEPELRERVEDVVLNRRPDAAERLLDIAEAAKGAAKDDSKKLEWRQAPV
                     RERLTHALVHGITDFITEDTEQAYRQILQDGGRPLHVIEGPLMDGMNVVGDLFGAGKM
                     FLPQVVKSARVMKQAVAHLLPYIEAEKQALQAAGADVRSKGKIVIATVKGDVHDIGKN
                     IVTVVLQCNNYEVVNMGVMVPAHEILAKAREEGADIVGLSGLITPSLEEMQHVAGEMQ
                     KDAYFRERQIPLLIGGATTSRVHTAVKIAPHYDGPVVYVPDASRSVGVAQGLLGEGVA
                     KFWADVRADYERVRQLHAGKKQAPLWPIDKARANKARIDWASYTPPVPKFIGQRLFTH
                     IDLAELLPFMDWTPFFQTWDLAGKYPAILNDEVVGQEATRVFQDAQAMLRKIVDGRWL
                     TANAVVGFWPANTVRDDDIELYADEARRDVVLTWHGLRQQTEKQEDAAGQLRPSRCLA
                     DFIAPRNAISSGATDAHPSGAGGQNIVKDYLGAFAVTAGIGVEQKVAEFMAEHDDYSA
                     ITLKALADRLAE"
BASE COUNT          384 a          709 c          704 g          287 t
ORIGIN      
        1 ggagcgtgat cgaggccggc ctgaaatgcc tgcagggcaa gggcatcgtc aactccatca
       61 gcatgaagga gggcgtggcc gagttcaagc gccaggccac gctgatccgc cgctacggcg
      121 ccgccgccgt ggtgatggcc ttcgacgagc agggccaggc cgacaccttc gcgcgcaaga
      181 tcgagatctg cgagcgcgcc taccgcgtgc tggtcgacga ggtgggcttc gcgcccgagg
      241 acatcatctt cgaccccaac atcttcgcca tcgccaccgg catcgaggag cacgccaact
      301 acgcggtgga cttcatcgag gcggtgcgct ggatcaagca gcacctgccc ggcgccaagg
      361 tgtcgggcgg cgtgtccaac gtgtctttca gcttccgcgg caacgacccg gtgcgcgagg
      421 ccatccacac cgtgttcctc tatcacgcca ttcaagccgg catggacatg ggcatcgtca
      481 acgccggcat ggtgggcgtg tacgacgacc tggagcccga gctgcgcgag cgcgtggaag
      541 acgtggtgct caaccgccgc cccgacgccg ccgagcgcct gctggacatc gccgaggccg
      601 ccaagggcgc ggccaaggac gattcgaaga agctggagtg gcgccaggcc ccggtgcgcg
      661 agcgcctgac gcacgcgctg gtgcacggca tcaccgactt catcaccgaa gacaccgaac
      721 aagcctaccg gcagatcctg caggatggcg ggcgcccgct gcacgtcatc gagggcccgc
      781 tgatggacgg catgaacgtg gtcggcgacc tgttcggcgc cggcaagatg ttcctgccgc
      841 aggtggtcaa gagcgcgcgc gtgatgaagc aggccgtggc ccacctgctg ccctacatcg
      901 aggccgagaa gcaggcgctg caggccgccg gcgccgacgt gcgcagcaag ggcaagatcg
      961 tgatcgccac cgtcaagggc gacgtgcacg acatcggcaa gaacatcgtc accgtggtgc
     1021 tgcagtgcaa caactacgag gtcgtgaaca tgggcgtgat ggtgcccgcg cacgaaatcc
     1081 tggccaaggc gcgcgaggaa ggcgccgaca tcgtcggtct gtcgggcctg atcacgccca
     1141 gcctggagga aatgcagcat gtggccggcg aaatgcagaa ggacgcctac ttccgcgaac
     1201 gccagattcc gctgctgatc ggcggcgcca ccaccagccg cgtgcacacc gcggtcaaga
     1261 tcgcgccgca ctacgacggc ccggtggtct acgtgcccga cgcctcgcgc agcgtgggcg
     1321 tggcgcaggg cctgctgggc gagggcgtgg cgaagttctg ggccgacgtg cgcgccgact
     1381 acgagcgcgt gcgccagctg cacgccggca agaagcaggc gccgctgtgg ccgatcgaca
     1441 aggcgcgcgc caacaaggcg cgcatcgact gggccagcta caccccgccg gtgcccaagt
     1501 tcatcggcca gcgcctgttc acccacatcg acctggccga gttgctgccc ttcatggact
     1561 ggacgccctt cttccagacc tgggacctgg ccggcaagta cccggccatc ctgaacgacg
     1621 aagtggtggg ccaggaagcc acgcgcgtgt tccaggacgc gcaggccatg ctcaggaaga
     1681 tcgtcgacgg ccgctggctg accgccaacg cggtggtcgg cttctggccc gccaataccg
     1741 tgcgcgacga cgacatcgag ctctacgccg acgaggcgcg ccgcgacgtc gtgctgacct
     1801 ggcacggcct gcgccagcag accgaaaagc aggaggacgc cgccggccag ctgcgcccga
     1861 gccgctgcct ggcggacttc atcgccccgc ggaatgctat aagttcagga gctactgacg
     1921 ctcacccctc gggcgctgga ggccagaata tcgtcaagga ttatctgggc gccttcgccg
     1981 tcaccgccgg catcggggtg gaacagaagg tggccgagtt catggccgag cacgacgact
     2041 acagcgccat cacgctcaag gccctggccg accgcctggc cgag
//
LOCUS       DAOOOG010000320         2080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_67335_length_2080_cov_30.956049, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000320 DAOOOG010000000
VERSION     DAOOOG010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67335_length_2080_cov_30.956049"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1635)
                     /locus_tag="PK242_13920"
     CDS             complement(97..1635)
                     /locus_tag="PK242_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HOZ95164.1"
                     /translation="MPPSLRHAARVLLLALPWLWPFTSGPTAATQPYLAGAALAALLL
                     LAWPRQRQAGWHALLDGWLLAALVSSAIALLQYFNLEAPLFPWVNQAQTGQAFGNLRQ
                     PNQLATLLVIGLLALAWQAQAGRLSPRAAAALGVPLLLALAASASRVGALELLLLGAL
                     CLWWARGSGQLRRAGALLAAAWLLYALAALALPWALQLSEGQQGRDLIERLRAGEGTC
                     GSRLILWRNVLHLIALRPWTGWGWGELDWAHYITLYPGERFCHILDHAHNLPLHLAVE
                     LGLPLTLLAAGVLAWAVWRARPWAERQPARQLAWAVLAAIGLHSLVEYPLWYAPFQIA
                     AACCLWLLWSTRRGAPAEAGPAPGRRLLAVLLLAGVGCAGWDYHRISQIYLPREQRAA
                     AYRDDAMAHARRSWLFADAVRFAEVTTRPPTRDNAAWLLPAALQTLHYSPEPRVITQV
                     IESASLLGRDELALTHLARFRAAFPREYRQWSADNRQLLQDAQAQQRAAGSAPLAPAS
                     AMNK"
     gene            complement(1647..>2080)
                     /gene="moaC"
                     /locus_tag="PK242_13925"
     CDS             complement(1647..>2080)
                     /gene="moaC"
                     /locus_tag="PK242_13925"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOZ95165.1"
                     /translation="PATHRVAVAGGRIEMQPATLALIESGNAKKGDVLGIARLAGIMA
                     AKKTSELIPLCHPLALSRVAIEFEASGADGTGSAAVFCTATAETTGPTGVEMEALTAV
                     QVALLTIYDMCKAADRGMTITGVRLLEKRGGKSGHFVAARA"
BASE COUNT          302 a          782 c          754 g          242 t
ORIGIN      
        1 cgacagcctc ctaggatgac gcgcgacccg gcggcgggcc cgtgtcgtag aagtgcgaaa
       61 taggcctgca gcgcccgccc atcctgcgcc gacagctcac ttgttcatag cgcttgcggg
      121 cgccagcggg gcgctgccgg cggcgcgctg ctgggcttgc gcgtcctgca gcagctgccg
      181 gttgtcggcc gaccactgac ggtattcgcg cgggaaggcg gcgcgaaagc gcgccaggtg
      241 cgtcagcgcc agctcgtcgc gccccagcag gctggcactt tcgatcacct gggtgatgac
      301 gcgcggctcg ggcgagtaat gcagcgtctg cagcgccgcc ggcagcagcc aggcggcgtt
      361 gtcgcgcgtc ggcggtcggg tggtcacctc ggcaaagcgc accgcgtcgg caaacagcca
      421 ggagcggcgc gcatgcgcca tggcgtcgtc gcggtaggcg gcggcgcgct gctcgcgcgg
      481 caggtagatc tggctgatgc ggtggtagtc ccagccggcg cagcccacgc cggccagcag
      541 cagcaccgcc agcagccgcc ggcccggcgc cggcccggcc tcggccggcg caccgcgccg
      601 cgtggaccac agcagccaca ggcaacacgc cgccgcgatc tggaacggcg cataccacag
      661 cgggtactcc accaggctgt gcaggccgat ggcggccagc accgcccagg ccagctggcg
      721 cgccggctgg cgctcggccc agggccgggc gcgccacacc gcccaggcca gcacgcccgc
      781 ggccagcagg gtcagcggca ggcccagctc caccgccagg tgcagcggca ggttgtgtgc
      841 gtggtccagg atgtgacaga agcgctcgcc cgggtacagg gtgatgtagt gcgcccagtc
      901 cagctcgccc cagccccagc cggtccaggg ccgcagggcg atcaggtgca gcacgttgcg
      961 ccacaggatc aggcggctgc cgcaggtgcc ttcgccggcg cgcaggcgct cgatcaggtc
     1021 gcgtccctgc tggccctcgc tcagctgcag cgcccacggc agcgccagcg ccgccagcgc
     1081 gtacagcagc caggccgccg ccagcagcgc gcccgcgcgg cgcaactgcc cgctgccgcg
     1141 cgcccaccac aggcacagcg cgcccagcag cagcagctcg agcgcgccca cgcgcgaggc
     1201 gctggccgcc agcgccagca gcagcggcac gcccagcgct gccgccgctc gcgggctcag
     1261 ccggccggct tgcgcctgcc aggccagcgc cagcagcccg atcaccagca gggtggccag
     1321 ctggttgggc tggcgcaggt tgccgaaggc ctgcccggtc tgcgcctggt tgacccaggg
     1381 aaacagcggc gcttccaggt tgaagtactg cagcagggcg atggcgctgc tgaccagcgc
     1441 cgccagcagc cagccgtcca gcagcgcgtg ccagccggcc tggcgctggc gcggccaggc
     1501 cagcagcagc agcgccgcca gcgccgcgcc cgccaggtag ggctgggtgg ccgccgtcgg
     1561 gccggaggtg aacggccaca gccagggcag cgccagcagc agcacccgcg cggcgtgacg
     1621 gagcgaaggc ggcacggatg ggcggctcag gcgcgcgcgg cgacgaaatg gcccgacttg
     1681 ccgccacgct tttccagcag gcgcacgccg gtgatggtca tgccgcggtc ggccgccttg
     1741 cacatgtcgt agatggtcag cagcgccacc tgcaccgcgg tcagcgcctc catctccacc
     1801 ccggtggggc cggtggtctc tgccgtggcg gtgcaaaaaa cggccgcaga gcccgtccca
     1861 tctgcgccag aagcttcgaa ttcgatagca acacgggaca gcgccagcgg gtggcacagg
     1921 ggaatcagct cgctggtctt cttggccgcc atgatgccgg cgaggcgcgc gatgcccagc
     1981 acgtcgccct ttttcgcgtt gcccgattcg atcagcgcca gcgtcgccgg ctgcatctcg
     2041 atgcgcccgc cggccaccgc cacccggtgc gtggccggct
//
LOCUS       DAOOOG010000321         2064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_68139_length_2064_cov_39.791936, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000321 DAOOOG010000000
VERSION     DAOOOG010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68139_length_2064_cov_39.791936"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1910)
                     /locus_tag="PK242_13930"
     CDS             complement(<1..1910)
                     /locus_tag="PK242_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HOZ95166.1"
                     /translation="MTKTLPRLHGLALAAIASAALLACGGSDDRFNDKPAGIGALHTQ
                     HYDGSSDDLLTAGLGASGLASALPPSYADPLQPTAAELRRAAIYNSYRALVDTTAAGG
                     YGVFYGPGVTVEGTAGGGEGKVPGSETLAFSDDGSGRENVTLMVQVPDSFDPARACIV
                     TATSSGSRGVYGAISTGEWGLKRGCAVAYTDKGTGSAAHDLRTDTVPRIDGTRTAAGS
                     AAGQAAFDAGLTAAELAAFNAATPDRLAFKHAHSGRNPEKDWGRHTLQAVRLALYVLN
                     ERYGELDASGRRRVRLHTGNTLVIASSVSNGGGAALAAAEDDREGLIDGVAVAEPAIE
                     MPANAGVAVQRGASQVPVIGRNLIDFTSYANLYQACAALAPSQAAAPFGAAFASLYNN
                     PALPIAANRCAALAQRGLLNGSSTAEQAEHALQRLREYGWEAESSVLQASHAAFEIAP
                     AVAVTFANALARARVTDHLCGFSYAATAANGSVTTLAPALLAGMFATGNGVPPSSGVN
                     LVNNLAAQGPARDFLSFTAQGVADWNLDGALCLRELVTGQGTLAARLQTGMRETQRHG
                     NLHGKPTLIVHGRDDALLPVNHTSRPYAALNRKVEGAASRLSYIEVTNAQHFDGFIGL
                     PAVLPGYDSRYVP"
     gene            complement(1945..>2064)
                     /locus_tag="PK242_13935"
     CDS             complement(1945..>2064)
                     /locus_tag="PK242_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ95167.1"
                     /translation="LLSPDRWLLWLGLLFVLSVYYFPTGVVGRLRTWRERLVR"
BASE COUNT          256 a          697 c          810 g          301 t
ORIGIN      
        1 ggcacgtagc ggctgtcgta gccgggcagc acggcgggca ggccgatgaa gccgtcgaaa
       61 tgctgggcgt tggtgacctc gatgtagctc agccgactgg ccgcaccttc gaccttgcgg
      121 ttcagcgccg cgtaggggcg cgaggtgtgg ttgaccggca gcagggcgtc gtcgcgcccg
      181 tgcacgatca gcgtcggctt gccgtgcagg ttgccgtggc gctgggtttc gcgcatgccg
      241 gtctgcaggc gcgcggccag cgtgccctgg cccgtgacca gctcgcgcag gcacagcgcg
      301 ccgtcgaggt tccagtcggc cacgccctgg gcggtgaagc tcaggaaatc gcgcgccggg
      361 ccctgggccg ccaggttgtt gaccaggttg acgccgctgg acggcggcac gccgttgccg
      421 gtggcgaaca tgccggccag cagtgccggc gccagcgtgg tgacgctgcc gttggcggcg
      481 gtggcggcgt agctgaagcc gcacaggtgg tccgtcacgc gggcgcgcgc cagggcgttg
      541 gcaaaggtga cggcaacggc gggcgcgatc tcgaacgctg cgtgcgaggc ctgcagcacg
      601 ctggactcgg cctcccagcc gtactcgcgc aggcgctgca gcgcgtgttc ggcctgttcg
      661 gcggtgctgc tgccgttcag cagcccgcgc tgcgccagcg cggcgcagcg gttggcggcg
      721 atcggcagcg ccgggttgtt gtagaggctg gcgaaggccg cgccgaacgg tgccgctgcc
      781 tgcgagggcg ccagcgcggc gcaggcctgg tagaggttgg cgtagctggt gaaatcgatc
      841 aggttgcgac cgatcaccgg cacctggctg gcgccgcgct gcaccgccac gccggcgttg
      901 gcgggcattt cgatcgccgg ctcggccacc gccacgccgt cgatcaggcc ttcgcgatcg
      961 tcttcggccg cagccagggc ggcgccgccg ccgttcgaca cgctggaggc gatcaccagc
     1021 gtgttgccgg tgtgcaggcg cacgcggcgg cggccgctgg cgtcgagctc gccatagcgc
     1081 tcgttgagca cgtacagcgc cagccgcacg gcctgcaggg tgtggcggcc ccaatccttc
     1141 tcggggttgc ggcccgaatg ggcgtgcttg aacgccaggc gatcgggggt ggcggcgttg
     1201 aaggcggcca gctcggccgc ggtcagcccg gcgtcgaagg ccgcctggcc ggcggcgctg
     1261 ccggccgcgg tgcgcgtgcc gtcgatccgc ggcacggtgt cggtgcgcag gtcatgggcg
     1321 gcgctgccgg tgcccttgtc ggtgtaggcc accgcgcagc cgcgcttgag gccccattcg
     1381 ccggtggaga tggcgccgta cacgccgcgc gagccggagg acgtggcggt gacgatgcag
     1441 gcacgcgccg ggtcgaagct gtcgggcacc tgcaccatca gggtgacgtt ctcgcggccc
     1501 gagccgtcgt cgctgaaggc cagggtctcg ctgccgggga ccttgccttc gccaccgccc
     1561 gccgtgcctt cgaccgtcac gccggggccg tagaacacgc cgtagccgcc ggccgccgtg
     1621 gtgtcgacca gggcgcggta gctgttgtag atggcggcgc ggcgcagctc ggccgcggtc
     1681 ggctgcagcg ggtcggcgta gctgggcggc agcgccgacg ccagcccgct ggcgcccagg
     1741 ccggcggtca gcagatcgtc gctgctgccg tcgtagtgct gcgtgtgcag cgcgccgatg
     1801 cctgccggtt tgtcgttgaa ccggtcgtcg ctgccgccgc aggccagcag ggccgcactg
     1861 gcgatggcgg ccagtgccag gccgtgcagg cgcgggaggg tcttggtcat ggcttgtctc
     1921 ctctcggggt ggtgaaaacc ggggtcagcg caccaaccgc tcgcgccagg tgcgcaggcg
     1981 ccccaccacg ccggtgggga agtaatagac cgacagcacg aacagcaggc ccagccacag
     2041 cagccagcgg tcgggcgaca gcag
//
LOCUS       DAOOOG010000322         2029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_69860_length_2029_cov_34.206180, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000322 DAOOOG010000000
VERSION     DAOOOG010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69860_length_2029_cov_34.206180"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PK242_13940"
     CDS             <1..398
                     /locus_tag="PK242_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOZ95168.1"
                     /translation="AMLQAGVNGPFGAVVRAVTGSDGQPIPAMYFWATGTLSAFLDNA
                     PTYLVFFNTAGGDAQQLMGPLASTLAAISAGSVFMGAITYIGNAPNLMVKAIAEDRGV
                     RMPSFFGYMAWSVGILVPLFIVLTLVFFR"
     gene            565..1221
                     /locus_tag="PK242_13945"
     CDS             565..1221
                     /locus_tag="PK242_13945"
                     /inference="COORDINATES: protein motif:HMM:NF041539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HOZ95169.1"
                     /translation="MRRIFGKVIFFGLLVLSSMGVVHAQILYSTLPADPDGGPCFGGG
                     NLAAVEMATPTGAPYQINGATVRMHHADDAAASFTVAVYTNNAGVPGTLVGTVGTAAG
                     NGLGTMDLYNLTPASPIALSASTNYWVVASSTSADTCAFGWTFNASTPSGIFTYVAET
                     QFFGGSWDDRTGEHFALELNGQVVAPAGSIAPVPTLSEWALVLMLSLVAFMAARRLRR
                     "
     gene            complement(1386..>2029)
                     /locus_tag="PK242_13950"
     CDS             complement(1386..>2029)
                     /locus_tag="PK242_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HOZ95170.1"
                     /translation="GAAMADIRADRLPPAFKKLARASRIFDQLVNAWDVLATMTPPEY
                     TAIRPYLGPSSGFQSYQYRCIEFALGNKNAAMLKVHAHKPERLALVQSYYDAPSLYDE
                     ALRLMARRGLAVPASHTARDWTQPYEASDAVEDAWLSVYRDPEHHWDLYQLGEKLTDL
                     EDAFRVWRFKHVTTVERIIGFKRGTGGTSGVGYLRKMLDVVLFPELWRLRTDL"
BASE COUNT          320 a          632 c          691 g          386 t
ORIGIN      
        1 tcgccatgct gcaggcgggc gtcaacggcc cgttcggcgc cgtggtgcgc gccgtcactg
       61 gcagcgacgg ccagccgatc ccggccatgt acttctgggc gacgggcacg ctgagcgcgt
      121 tcctggacaa cgccccgacc tacctggtgt tcttcaacac cgccggcggc gacgcgcagc
      181 agctgatggg cccgctggcc agcacgctgg cggccatcag cgccggctcg gtgttcatgg
      241 gcgccatcac ctacatcggc aacgcgccca acctgatggt caaggccatc gccgaggacc
      301 gcggcgtgcg catgcccagc ttcttcggct acatggcctg gagcgtgggc atcctggtgc
      361 cgctgttcat cgtgctgacg ctggtgttct tccgctgagc tgagcgtggc tcagcgggcg
      421 gcgcgacggg gcgccggcca cagcaccgag gcgatggcca cgcccatgag aaaaaggccg
      481 cctacctcat ggtttctcta tggggttgcc gttatatttc aatgtcgtta cgtgtgacgg
      541 tgttttgctc taggaggaaa ggtgatgagg aggatttttg ggaaggtcat tttctttggc
      601 ctgcttgtgt tgtcttcaat gggggtggtg catgcacaga tcttgtacag cacgctgccg
      661 gccgatccgg acggtgggcc ctgcttcggc ggcggcaatc tggccgccgt cgagatggcg
      721 accccaacgg gagcgcccta ccaaatcaac ggcgccacag tgcgcatgca ccacgcggac
      781 gatgctgcgg caagcttcac cgtggcggtc tataccaaca acgccggggt gcccggcact
      841 ctggtgggta cggtggggac ggccgctggc aatggcttgg ggacgatgga tctgtacaac
      901 ctgacgccgg catcacccat tgccttgtcg gcttccacca attactgggt ggtggccagc
      961 agcacatctg ccgatacatg cgcgtttggc tggaccttca acgcatcgac gccgagcggc
     1021 atcttcacct atgtggccga aacccagttt tttggtggaa gttgggacga tcgaaccggt
     1081 gagcatttcg cacttgaact caacggccag gtcgtcgcgc ccgccggcag cattgctcca
     1141 gttccaacct tgtccgaatg ggcgctggtt ttgatgctgt cgctggtggc gttcatggcc
     1201 gcgcggcgct tgcgccgata gacggcgctc acgccgggtt ccgccggctg tcaacgttca
     1261 catggccaca gccttacccc aaccgcgctt gcaaacgggg atgggcttgg gaccgaaccg
     1321 gtagctgacg gggcgtcgaa agacctctcg ccaggcgcgc gatgtgcacg gatgtgctgc
     1381 cgcagttaca gatccgtgcg cagccgccac agctccggga acagcaccac gtccagcatc
     1441 ttgcgcagat agcccacgcc gctggtgccg ccggtgccgc gcttgaagcc gatgatgcgc
     1501 tccacggtgg tgacgtgctt gaaacgccag acgcggaagg cgtcttccag gtcggtcagc
     1561 ttttcgccca gctggtagag gtcccagtgg tgttcggggt cgcggtagac gcttagccag
     1621 gcgtcttcga ccgcgtcgct ggcttcatag ggctgggtcc agtcgcgcgc cgtgtgactg
     1681 gcgggcaccg ccaggccgcg gcgcgccatc aggcgcagcg cctcgtcgta cagcgagggc
     1741 gcgtcgtagt acgactgcac cagcgccagc cgctcgggct tgtgcgcgtg caccttcagc
     1801 atggcggcgt tcttgttgcc cagggcgaat tcgatgcagc ggtactggta gctctgaaag
     1861 ccgctggacg ggcccaggta ggggcggatg gcggtgtact cgggtggcgt catggtggcc
     1921 agcacgtccc aggcattgac cagctgatcg aagatgcggc tggcgcgcgc cagtttcttg
     1981 aaggccggcg gcaggcggtc ggcgcggatg tcggccatgg cggcgccga
//
LOCUS       DAOOOG010000323         2028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_69911_length_2028_cov_21.076533, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000323 DAOOOG010000000
VERSION     DAOOOG010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69911_length_2028_cov_21.076533"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PK242_13955"
     CDS             <1..1124
                     /locus_tag="PK242_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOZ95171.1"
                     /translation="AVPMNGAAKAFEVEGTIGGSSQITNGNDQLTLEYTALRVINVEN
                     LSEAGRGSGADVRKVDLGQALTQRLGAADKTVTQRELRNVGPSITYKLRDAAGQANEF
                     HNYMLPVDMGDGGPVFLLGVRASQAEGFRYLRVPADEDGAIDGFVRLRGALQDAAQRE
                     QAVRRYVRKAVDPQRPELVEQLATSAARALGLFAGAEAVKGSTTGGLQAVSDFIDTAV
                     PEGERERAAEVLLRILNGSLLELNALAREQQGKPPLPEDEATRRFMTQAVLALSDAPL
                     YPAPVAFELTGFEQVQASVFQVARAPGQNVVYLGCLLLILGVFAMLYVRERRLWVWLA
                     PSDTGAHATMALSSNRKLLDIDREFDQWQRRLLHRESNP"
     gene            1121..>2028
                     /locus_tag="PK242_13960"
     CDS             1121..>2028
                     /locus_tag="PK242_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HOZ95172.1"
                     /translation="MTISVATVNPTTVTLGGGYFARRTWGDWLFALLVVAGAVFAFQR
                     YHASMDIYEKWILAGTAPVLIGLGWFWRPLRTLMLVVAGLSLLAVVSYGGDLRRADSV
                     FWLKYFLSSQSAILWMSMIFFMSTVFYWLGMFSRGQSEALEGIGSKLLWVAVTLALVG
                     TLVRWYEGHQMGPDIGHIPVSNLYEVFVMFCWMTALFYLYYESQYRTRAMGAFVMLVV
                     SAAVGFLLWYTLVREAHEIQPLIPALQSWWMKLHVPANFIGYGTFALAAMLGFAYLIK
                     QQAEETRWYKLAPIWVFGIALCFVPIA"
BASE COUNT          290 a          668 c          726 g          344 t
ORIGIN      
        1 gcgccgtgcc catgaacggc gcggccaagg cgttcgaggt cgagggcacc atcggcggca
       61 gctcccagat caccaacggc aacgaccaac tgacgctcga atacacggcg ctgcgcgtga
      121 tcaacgtcga gaacctgagt gaggccggcc gcggctcggg cgccgacgtg cgcaaggtcg
      181 acctcggcca ggcgctgacg cagcgcctgg gcgcggccga caagaccgtg acgcagcgcg
      241 agttgcgcaa cgtcggcccc agcatcacct acaagctgcg cgacgcggcg ggccaggcca
      301 acgagttcca caactacatg ctgccggtcg acatgggcga cggcgggccg gtgttcctgc
      361 tgggcgtgcg cgccagccag gccgagggct tccgctacct gcgcgtgccg gccgacgagg
      421 acggcgccat cgacggcttc gtgcgcctgc gcggcgcgct gcaggacgcc gcccagcgcg
      481 agcaggcggt gcgccgctac gtgcgcaagg cggtcgatcc gcagcggccc gagctggtcg
      541 agcagctggc cacctcggcg gcgcgcgcgc tgggcctgtt cgcgggggcc gaggcggtca
      601 agggcagcac gaccggcggc ctgcaggcgg tgtcggactt catcgacacg gcggtgcccg
      661 agggcgagcg cgagcgcgcc gccgaggtgt tgctgcgcat cctcaacggc agcctgctgg
      721 agctgaacgc gctggcgcgc gagcagcagg gcaaaccgcc gctgcccgag gacgaggcca
      781 cgcgccgctt catgacgcag gcggtgctgg cgctgtccga cgcgccgctg tacccggcgc
      841 cggtggcgtt cgagctgacc ggcttcgagc aggtgcaggc cagcgtgttc caggtggcgc
      901 gagccccagg ccagaatgtg gtctatctgg gctgcctgtt gctgatcctg ggggttttcg
      961 ccatgctgta cgtgcgcgag cggcgcctgt gggtgtggct ggcgcccagc gacaccggcg
     1021 cgcacgccac gatggcgttg tcgtccaacc gcaagctgct cgacatcgac cgcgaatttg
     1081 atcagtggca gcgcaggctg ctgcacaggg agtccaaccc atgactattt ccgtcgctac
     1141 cgtcaacccg accaccgtca cgctgggcgg cggttatttc gcgcgtcgca cctggggtga
     1201 ctggctgttc gccctgctgg tggtggccgg cgcggtgttc gccttccagc gctaccacgc
     1261 gtcgatggac atctacgaga agtggatcct ggccggcacc gccccggtgc tgatcgggct
     1321 gggctggttc tggcgcccgc tgcgcaccct gatgctggtg gtggcgggcc tgtcgctgct
     1381 ggccgtcgtc tcctacggcg gtgacttgcg gcgcgccgat tcggtgttct ggctcaagta
     1441 cttcctgtcc agccagtcgg ccatcctgtg gatgagcatg atcttcttca tgagcacggt
     1501 gttctactgg ctcggcatgt tctcgcgtgg ccagagcgag gcactggagg gcatcggcag
     1561 caagctgctg tgggtggcgg tgacgctggc gctggtgggc accctggtgc gctggtacga
     1621 aggccaccag atggggccgg acatcggcca catcccggtc agcaacctgt acgaagtgtt
     1681 cgtcatgttc tgctggatga cggcgctgtt ctacctctac tacgagtcgc agtaccgcac
     1741 ccgcgccatg ggcgccttcg tcatgctggt ggtcagcgcg gcggtcggct ttctgctgtg
     1801 gtacacgctg gtgcgcgagg cgcacgagat ccagccgctg atcccggcgc tgcaaagctg
     1861 gtggatgaag ctgcacgtgc cggccaactt catcggctac ggcaccttcg cgctggccgc
     1921 catgctgggc tttgcctacc ttatcaagca gcaggccgag gagacgcgct ggtacaagct
     1981 ggcgccgatc tgggtgttcg gcatcgcgct gtgcttcgtg ccgatcgc
//
LOCUS       DAOOOG010000324         2026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_70015_length_2026_cov_21.570776, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000324 DAOOOG010000000
VERSION     DAOOOG010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70015_length_2026_cov_21.570776"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="PK242_13965"
     CDS             <1..815
                     /locus_tag="PK242_13965"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HOZ95173.1"
                     /translation="DPSWENHRALFTQAGFEVGTYRYYDAATRSLNFDGMLADLQAAA
                     AGTIVVLHACCHNPTGYDITPAQWDQVIAAVKARGLTPFLDMAYQGFAEGIAEDGAVI
                     GKFVAAGLTFFVSTSFSKSFSLYGERVGALSVLCASKDEAARVLSQLKIMIRTNYSNP
                     PTHGGAVVAMVLGDPKLRAQWEQELAGMRTRIKAMRRKLVDGLKAAGVKQDMSFITTQ
                     VGMFSYSGLSKEQMVRLRNEFGVYGTDTGRMCVAALNEHNIDYVCQAIAKVI"
     gene            complement(919..1917)
                     /locus_tag="PK242_13970"
     CDS             complement(919..1917)
                     /locus_tag="PK242_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOZ95174.1"
                     /translation="MTDTPPPCVLERWSSAEVDAAIRLDYWRDVAHNWVEVQPLSSGD
                     RLNASWSLLRGESSFFGTKRSTAYEMRTHARHVPPGEDMVVLSLLEVGEMRLNAAPGE
                     HQRVTAGMLGMYAPRQDACYRWGDGARQTYLALPRSVVFDALGSEPRNLLLAPQRCAL
                     APMLTSQLVHLGRLARQPGHVGADEYASLLAATRALALLTLRNLGRQGDAGDLPDVTE
                     SLHAGRRAAALRFMESHAHRHDLDAGAIARGAGCSRTRLYEAFAEQDRSVMDTLREIR
                     LQRARTLIERTVRLHVGLVAWRCGFADQSGFSKLFKARFGITPSEWHRWAQGRRGG"
     gene            complement(1923..>2026)
                     /locus_tag="PK242_13975"
     CDS             complement(1923..>2026)
                     /locus_tag="PK242_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004545773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOZ95175.1"
                     /translation="RCGFADQSAFSKLFRARFGLTPTEWRVRRAGQG"
BASE COUNT          346 a          720 c          662 g          298 t
ORIGIN      
        1 gcgacccgag ctgggaaaac caccgcgccc tgttcaccca ggccggcttc gaggtaggca
       61 cctaccgcta ctacgacgcc gccacgcgca gcctgaactt tgacggcatg ctggccgacc
      121 tgcaggctgc cgccgccggc accatcgtgg tgctgcacgc ctgctgccac aaccccaccg
      181 gctacgacat cacccccgcg cagtgggatc aggtgatcgc cgcggtcaag gcgcgcggcc
      241 tcacaccctt cctcgacatg gcctaccagg gctttgccga gggcatcgcc gaggacggcg
      301 cggtgatcgg taagttcgtc gccgccggcc tgaccttctt tgtctcgacc tcgttttcca
      361 agagcttttc gctctacggc gagcgcgtgg gcgcgctgtc ggtgctgtgc gccagcaagg
      421 acgaagccgc gcgcgtgctc agccagctca agatcatgat ccgcaccaac tacagcaacc
      481 cgcccacgca cggcggcgcg gtggtggcca tggtgctggg cgaccccaag ctgcgcgcgc
      541 aatgggagca ggaactggcc ggcatgcgca cccgcatcaa ggccatgcgc cgcaagctgg
      601 tcgatggcct gaaggccgcg ggcgtgaagc aggacatgag cttcatcacc acccaggtcg
      661 gcatgttcag ctattcgggc ctgtccaagg agcagatggt gcgcctgcgc aacgagtttg
      721 gcgtgtacgg cacggacacc ggccgcatgt gcgtggcggc gctgaacgag cacaacatcg
      781 actacgtgtg ccaggcgatc gccaaggtga tctgagcgcc gcgcggtcag ctatttgatt
      841 gatagctgac cgcgcaggaa tggtcccggc tggggccatt ttttcatgaa ctgataacca
      901 tcgggcgcgg cccaggcctc aaccgccgcg cctgccctgc gcccagcggt gccactccga
      961 cggcgtgatg ccgaagcgcg ccttgaacag cttgctgaac cccgactggt cggcaaagcc
     1021 gcagcgccag gctaccaggc ccacatgcag gcgcacggtg cgctcgatca gggtgcgtgc
     1081 gcgttgcaag cggatctcgc gcagcgtgtc catcaccgac cgatcctgtt cggcgaacgc
     1141 ctcatacagg cgcgtgcgcg aacaacccgc gccgcgcgca atggcgccgg catccagatc
     1201 gtgccggtgt gcatggcttt ccatgaagcg cagtgccgcg gcgcgacggc cggcgtgcag
     1261 gctttcggtc acatcgggca gatcgcccgc gtcgccctgg cgccccaggt tgcgcagcgt
     1321 cagcaacgcc agggcgcgcg tggcggccag caggctggcg tattcgtcgg caccgacgtg
     1381 accgggctga cgcgccagtc gccccaggtg cacgagctgg ctggtcagca tgggcgccag
     1441 tgcacaacgc tgcggcgcca gcagcagatt gcgcggctcg gagcccagcg cgtcgaacac
     1501 cacactgcgc ggcagcgcca gataggtctg gcgcgcgccg tcgccccagc ggtaacaggc
     1561 gtcctggcgc ggcgcgtaca tacccagcat gcccgccgtc acgcgctggt gctcgccggg
     1621 cgccgcattc agccgcatct cgccgacttc cagcagcgac agcaccacca tgtcctcgcc
     1681 cggcggtacg tggcgagcat gggtgcgcat ctcgtaggcg gtggagcgct tggtgccgaa
     1741 aaaactgctc tcgccgcgca gcagtgacca cgacgcattc agccggtcac ccgacgacag
     1801 cggctgcacc tccacccagt tgtgcgccac gtcgcgccaa tagtcgagcc gaatcgcggc
     1861 atcgacctcg gcagaagacc agcgctccag cacgcagggc ggcggcgtgt ccgtcacggc
     1921 gcctatccct gaccagccct gcgcacgcgc cactcggtcg gcgtcaggcc aaagcgggcg
     1981 cgaaacagct tgctgaaggc cgactggtca gcgaagccgc agcgcc
//
LOCUS       DAOOOG010000325         2021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_70264_length_2021_cov_27.603255, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000325 DAOOOG010000000
VERSION     DAOOOG010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70264_length_2021_cov_27.603255"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..1090
                     /locus_tag="PK242_13980"
     CDS             338..1090
                     /locus_tag="PK242_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95176.1"
                     /translation="MKRTLRISAFVLAAGLATLSGCATKAPIPLAENFDLTVQPKVRS
                     AGHWELLSNDVVTQTLASLKNAQVPANTPMVVGLPANPSAFDRGFREFLITKLVQSGQ
                     TVYMQPDQAAAVAWQTQACAATPACRDQDALEVTYQTQVVRHDSPRPHFVPGQFTVLA
                     AGLMAAYGLRLEHLDAKLIATLGLAAAADYGASVYTGGPTNTELILTTTVSNRGRFVS
                     RKTDVYYLENVDAPLFMRSAPGYKAVNLKVVS"
     gene            1087..1659
                     /locus_tag="PK242_13985"
     CDS             1087..1659
                     /locus_tag="PK242_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HOZ95177.1"
                     /translation="MRKFAPLALTLALASLAGCSTFGDAAPVRRAGPTYETAAHNAFL
                     DSGRAAVGKLVEGLPADTLAGGPVLVATVVNVNNLTRSAPLGRTLSEQYASHMAAQGF
                     NVKEVKLRGELFVREGTGELLLSRELREIARSQNAAVVLVGTYSPASDYTYVSIKLVR
                     TEDSRILRGHDYALPNDRDVQRLLQEPSFR"
BASE COUNT          322 a          728 c          656 g          315 t
ORIGIN      
        1 tgcgggtggg tgcgtagcgt gctcacccag gaggtgaagg ccatcgcagc cgcgaaaccc
       61 cgcatcgatg cggtctgccc gacgttgaca agcggtcaac tgccgtttct aggttaaata
      121 tgcagcattt ggcggcaagg ccgggcggtg gcgactccgc cgcgcgcctg cgggctatcg
      181 caggcgctgt gaacaaaaac aacaaatcct gacttcctga tgcacttggc gcggcttctt
      241 gttgtgtcgc ctcaacagag tgcgcaaaat gtattcaaat gactccgttg gccggccgcc
      301 tctgtggctc ggctttgcac ctgttcgagg agaaaccatg aagcgtacat tgcgcatcag
      361 cgcgttcgtg ctggcggcgg gcctggcgac cctgtccggc tgtgccacca aggcacccat
      421 tcccctggcc gagaacttcg acctcaccgt gcagcccaag gtgcgctcgg ccggtcactg
      481 ggagctgctg tccaacgacg tggtcacgca gaccctggcc tccctgaaga acgcccaggt
      541 gccggccaac acaccgatgg tggtgggtct gccggccaac ccctcggcgt tcgaccgcgg
      601 ttttcgcgaa ttcctgatca ccaagctggt gcaaagcggc cagacggtct acatgcagcc
      661 cgaccaggcg gcggccgtcg cctggcagac gcaggcctgc gccgccaccc ccgcctgccg
      721 tgaccaggac gcgctggagg tcacctacca gacccaggtg gtgcgccacg acagcccgcg
      781 cccgcatttc gtgcccggtc agttcaccgt gctggcggcc ggtctgatgg ccgcctacgg
      841 cctgcgcctg gagcacctgg acgccaagct gatcgccacc ctgggcctgg ccgccgcggc
      901 cgactacggc gccagcgtgt ataccggcgg ccccaccaac accgagctga tcctgaccac
      961 cacggtcagc aaccgcggcc gcttcgtcag ccgcaagacc gacgtgtact acctggaaaa
     1021 cgtcgacgcg ccgctgttca tgcgctcggc gcccggttac aaggccgtca acctgaaggt
     1081 ggtgtcatga ggaagttcgc accccttgcc ctcaccctgg cgctggcctc gctggccggc
     1141 tgctccacct tcggtgatgc ggcgccggtg cgccgcgccg gccccaccta tgaaacggcg
     1201 gcccacaacg ccttcctcga cagcggccgc gcggccgtcg gcaagctggt cgaaggcctg
     1261 cctgccgaca ccctggctgg cggcccggtg ctggtggcca cggtggtcaa cgtcaacaac
     1321 ctgacgcgct cggccccgct gggccgcacg ctgtcggagc agtacgcctc gcacatggcg
     1381 gcgcagggct tcaacgtcaa ggaagtcaag ctgcgcggcg agctgttcgt gcgcgaaggc
     1441 acgggcgagc tgctgctgtc gcgcgagctg cgcgaaatcg cgcgcagcca gaacgccgcc
     1501 gtggtgctgg tgggcaccta ctcgcccgcc tccgactaca cctacgtcag catcaagctg
     1561 gtgcgcaccg aagacagccg catcctgcgc ggccacgact acgcgctgcc caacgaccgc
     1621 gacgtgcagc gcctgctgca ggaacccagc ttccgctgac ggcggcgcgg cgcctgcggg
     1681 cgcgctcctg ccgccggccg gccggccgcc ccatcggcgc gctcggtgcc cgcagccctg
     1741 ccggaccacc tgccggctgc gccttcggcg cctgcgccgc cgtgcgggtt gatcgggggt
     1801 tcggcatgcc ggcgggcgct ttatcatccg tgccagccgc cgcgatcggc ggggcgggca
     1861 cccgggcgca tcgtcacggt cgccctttca caggagagca aacgatgttg aacaacaaaa
     1921 agtcttgcgc cctgctgctg gccgctggtg cggcgctgct gttgagcggc tgcgaaacca
     1981 ccagcatgaa gatgggcagc ccggaggcca agaccgtggc c
//
LOCUS       DAOOOG010000326         2017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_70445_length_2017_cov_37.113660, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000326 DAOOOG010000000
VERSION     DAOOOG010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70445_length_2017_cov_37.113660"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PK242_13990"
     CDS             complement(<1..544)
                     /locus_tag="PK242_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOZ95178.1"
                     /translation="MSATLRFELPRPDIAAWRAGNTGVPGVWHFDSGQPGRALMITAL
                     IHGNELCGAWALKGLLEAGLRPARGTLTLAFCNLAAFDRFDAAHHDAARFVEQDLNRQ
                     WTPERLRAADSLERRRAAELAPFAERADWLLDLHSMHEPSAPLLLTGVQPRNLALARQ
                     LAAPEHIVIDAGHSDGVRLRD"
     gene            complement(541..1515)
                     /locus_tag="PK242_13995"
     CDS             complement(541..1515)
                     /locus_tag="PK242_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOZ95179.1"
                     /translation="MSPRQVLPLSALCVTALLAAPAVLAQGAYPSKPITLVVGYPPGG
                     STDLTGRTLGDALSRQLGVPVVIENLGGAGGTIGAQKVASAAPDGYTLLVGANNEVAI
                     SKLVTRTVKYDLKDFTPIGLIASQPMVLVASTTTGVKNLGEFIAKAKAAPGKYSYGSS
                     GVGTALHLAGEMVKERAGVFMTHIPYRGVAPLTSDLVGGNLEYGVFVLSSGLPHIKSG
                     KVVALGTTEARRSPLTPDIPALGEHPQLKGIDIGTWFVLMGPARLPEAVTTRLKTALA
                     EALKAPEFRQKMEASGSTVAAPGGDVQRFLASEVAKYQKIVQFAKIEQ"
     gene            complement(1625..>2017)
                     /locus_tag="PK242_14000"
     CDS             complement(1625..>2017)
                     /locus_tag="PK242_14000"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOZ95180.1"
                     /translation="CRTSNPGGDDLQNQRLADAPGQPLLYEHVARLAQGPWNLNGQLG
                     LVVGATYPAEIERVRQLAPSLPLLIPGVGAQGGDAVATVRAGLTAAGPIIVNSSRAIL
                     YASSGADFADAARTAARATRAQLQAARP"
BASE COUNT          269 a          681 c          768 g          299 t
ORIGIN      
        1 agtcgcgcag gcgcacgccg tcgctgtggc cggcgtcgat gacgatgtgc tcgggcgccg
       61 ccagctgccg tgccagcgcc agattgcgcg gctgcacgcc ggtgagcagc agcggggcgc
      121 tgggctcgtg catggaatgc agatccagca gccagtcggc gcgctcggca aacggagcca
      181 gttcggcggc gcggcgccgc tccagggagt cggcggcgcg caggcgctcg ggcgtccact
      241 ggcggttcag gtcctgctcg acgaagcggg cggcgtcgtg gtgcgcggcg tcgaagcggt
      301 cgaacgccgc caggttgcag aaggccagcg tgagcgtgcc gcgcgccggc cgcaggccgg
      361 cctccagcag gcccttgagc gcccaggcgc cgcacagctc gttgccgtga atcagggcgg
      421 tgatcatcag cgcgcgcccg ggctggccgc tgtcgaagtg ccacaccccg ggcaccccgg
      481 tgttgccggc gcgccaggcg gcgatgtcgg gtcgcggcag ctcgaagcgc aaggtggcgc
      541 tcattgctcg atcttggcga actgcacgat cttctggtac ttggccacct cgctggccag
      601 gaaccgctgc acgtcgccgc cgggcgccgc cacggtggag ccggaggcct ccatcttctg
      661 gcggaattcg ggcgccttga gcgcctcggc caacgcggtc ttcagacgcg tggtgacggc
      721 ctcgggcagc ctggccgggc ccatcagcac gaaccaggtg ccgatgtcga tgcccttgag
      781 ctgcgggtgc tcgcccagcg ccgggatgtc gggcgtgagc ggcgagcgcc tggcttcggt
      841 ggtgcccagg gccaccacct tgccgctctt gatgtgcggc aggccgctgg agagcacgaa
      901 cacgccgtac tccaggttgc cgcccaccag gtcgctggtc agcggcgcca cgccgcggta
      961 ggggatgtgg gtcatgaaca cgccggcgcg ctccttgacc atctcgccgg ccaggtgcag
     1021 cgccgtgccg acgcccgagc tgccgtagct gtacttgccg ggcgcggcct tggccttggc
     1081 gatgaactcg cccaggttct tcacgccggt ggtggtggaa gccaccagca ccatgggctg
     1141 cgaggcgatc aggccgatgg gggtgaagtc cttgaggtcg tacttgaccg tccgggtcac
     1201 cagcttgctg atggccacct cgttgttggc gcccaccagc agggtgtagc cgtccggcgc
     1261 ggcgctggcc accttctgcg cgccgatggt gccgccggcg ccgcccaggt tctcgatcac
     1321 caccggcacg ccgagctggc gcgacagcgc atcgcccagc gtgcggccgg tcaggtcggt
     1381 gctgccgcct ggcgggtagc cgaccaccag ggtgatcggc ttgctggggt aggcgccctg
     1441 cgccagcacg gccggcgcgg ccagcagggc ggtgacacac agggcgctca ggggcaggac
     1501 ttgacgtggg ctcatgggca gctttcttgg cgtggagggt gggcggtgaa caaggatcaa
     1561 aaacggcttt ggcgcttgtc agacgtgcgc caaaagctat tattaatata gcgcacgaca
     1621 gccctcaggg ccgggccgcc tgcagctgcg cgcgggtggc ccgggcggcg gtgcgggcag
     1681 cgtcggcaaa gtcggcgccg ctgctggcgt acaggatggc gcgcgagctg ttgacgatga
     1741 tggggccggc cgccgtcagc ccggcgcgca cggtggccac ggcgtcgcca ccctgcgcgc
     1801 ccacgccggg gatgagcagc ggcaggctgg gcgccagctg gcgcacgcgc tcgatctcgg
     1861 ccgggtaggt ggcgcccacc accaggccga gctggccgtt caggttccac ggcccctggg
     1921 ccaggcgcgc cacatgctcg tacagcagcg gctggccggg cgcgtcggcc aggcgctggt
     1981 tttgcaggtc gtcgccaccg gggttggagg tgcggca
//
LOCUS       DAOOOG010000327         1974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_72622_length_1974_cov_29.649297, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000327 DAOOOG010000000
VERSION     DAOOOG010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72622_length_1974_cov_29.649297"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1290)
                     /locus_tag="PK242_14005"
     CDS             complement(139..1290)
                     /locus_tag="PK242_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004545284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ95181.1"
                     /translation="MKPAPRPLFSPDHHAFREQVRRFCEREIAPQHAAWEEAHGVPRD
                     VWRQAGDHGLLCCWLPEALGGPGADLLFDFIVSEELGRIGATGPGFPLHSIIVAPYLA
                     AHGRPELQQALLPAMVRGDKIAAIAMTEPGTGSDVAAIRTQARREGEHYVLTGQKTFI
                     TNGHNADLVVVACKTDPAQGAHGVSLLVVERDMPGFTRGRNLRKIGQHAQDTAELFFD
                     QVRVPASHLLGDEGQGFKYLMQKLAQERLLISVQCQARAEAVLDWTVAYVQERRAFRQ
                     RLADFQNTRFKLAQLAADIAAGRAFCDQLIAQHLAGGLDAVGAAAGKLWHSELLGRVT
                     DECLQLFGGYGYMAEYPIARAYADARIERIYAGTSEIMKEIVARDLLDR"
     gene            complement(1364..>1974)
                     /locus_tag="PK242_14010"
     CDS             complement(1364..>1974)
                     /locus_tag="PK242_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ95182.1"
                     /translation="LLRNLHGLACWLVDSQIVLQGAQFPFAAPAHAELYARLFPGPVR
                     FGATCAGLRFDAHYLQLPLRRDGTALDQMLRRALPILVWPYRRDRLLSRRVRALLQQP
                     QAAHTAETLAARLALSARSLHRQLRAEGTSVQQLKDEARRAQACALLLRTDWPIARVA
                     RAAGFASDKSFARAFRHWTGHTPQGWRLHGDAESNRAPAPVE"
BASE COUNT          277 a          688 c          723 g          286 t
ORIGIN      
        1 gcagcctggg cttcgaggtc aaggcctacc cggaagaccc ggacttccgg ctggtcacgc
       61 acccgctctg aagcggcccg ggccccgcat gccgccgtgc gtcccggtcg ttcgtcattg
      121 cctgcgcgcc ggttcaggtc agcggtccag cagatcgcgc gccacgatct ctttcatgat
      181 ctccgaggtg ccggcgtaga tgcgctcgat gcgcgcatcg gcgtaggcgc gtgcgatcgg
      241 gtactcggcc atgtagccgt agccgccgaa cagctgcagg cattcgtcgg tgacgcggcc
      301 cagcagctcg ctgtgccaca gcttgccggc ggcggcgccg acggcatcca gcccgccggc
      361 caggtgctgc gcgatcagct ggtcgcagaa ggcgcggccg gcggcgatgt cggcggccag
      421 ctgggccagc ttgaaacggg tgttctgaaa gtcggccagg cgctggcgga acgcccggcg
      481 ctcctgcacg taggccacgg tccagtccag caccgcctcg gcgcgcgcct ggcactgcac
      541 gctgatcagc aggcgctcct gcgccagctt ctgcatcagg tacttgaagc cctggccctc
      601 gtcgcccagc aggtggctgg cgggcacgcg cacctggtcg aagaacagct cggccgtgtc
      661 ctgcgcgtgc tggccgatct tgcgcaggtt gcgcccgcgc gtgaagcccg gcatgtcgcg
      721 ctcgaccacc agcagcgaca cgccatgcgc gccctgcgcg gggtcggtct tgcaggccac
      781 caccaccagg tcggcgttgt ggccgttggt gatgaaggtc ttctggcccg tcagcacata
      841 gtgttcgccc tcgcgccgtg cctgggtgcg gatggccgcc acgtcgctgc cggtgccggg
      901 ttcggtcatg gcgatggcgg cgatcttgtc gccgcgcacc atggccggca gcagcgcctg
      961 ttgcagctcg ggcctgccat gcgcggccag gtacggcgcc acgatgatgg aatgcagcgg
     1021 aaagcccggc ccggtggcgc cgatgcgccc cagctcctcg ctgacgatga agtcgaacag
     1081 caggtcggcg cccggcccac ccagcgcctc cggcagccag cagcacaaca ggccgtggtc
     1141 gccggcctgg cgccagacgt cgcgcggcac gccgtgggct tcttcccagg cggcgtgctg
     1201 cggtgcgatc tcgcgctcgc agaagcggcg cacctgctcg cggaaggcgt ggtggtcggg
     1261 gctgaacagg gggcgggggg cgggtttcat gggctgcact gtaacccgac tgtcgggcca
     1321 cccggggcct gtcggaaaat atcagaatga tagctggcgg cgcttattcg acgggcgctg
     1381 gagccctgtt ggattcagca tcgccgtgca ggcgccagcc ctgcggcgtg tggccggtcc
     1441 agtggcggaa ggcgcgcgca aagctcttgt cgctggcaaa gccggccgca cgcgccacgc
     1501 gggcgatcgg ccagtcggtg cgcagcagca gcgcgcaggc ctgggcgcgc cgtgcttcgt
     1561 ccttgagctg ctgcacgctg gtgccttcgg cgcgcaactg gcggtgcagg ctgcgcgccg
     1621 acagcgccag ccgcgccgcc agcgtctcgg cggtatgcgc cgcctgcggc tgctgcagca
     1681 gggcgcgcac gcgccgcgac agcaggcggt cgcgccggta gggccagacc aggatcggca
     1741 gcgcgcggcg cagcatctgg tccagcgcgg tgccgtcgcg gcgcagcggc aactgcaggt
     1801 agtgggcgtc aaagcgcagc ccggcgcagg tggcgccgaa ccgcaccggg ccgggaaaca
     1861 gacgcgcgta caactcggca tgtgccggcg cggcgaacgg aaactgcgcg ccctgcagga
     1921 cgatctgcga atccaccagc cagcaggcca gcccgtgcag gttgcgcagc agcg
//
LOCUS       DAOOOG010000328         1951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_73916_length_1951_cov_20.607068, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000328 DAOOOG010000000
VERSION     DAOOOG010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73916_length_1951_cov_20.607068"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /gene="guaD"
                     /locus_tag="PK242_14015"
     CDS             complement(<1..1271)
                     /gene="guaD"
                     /locus_tag="PK242_14015"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HOZ95183.1"
                     /translation="MNAYRAELLRFDPHGAPLHDSDGLLVVGLGVDGVQRVIDAGPYR
                     SVAARHPQVPVTHWPGRLIAPGFIDMHVHFPQTDIIGSPADGLLPWLENYTFPAEARF
                     DSPAYAAEMANVFFAELLRHGVTTALTFATSHPVSVDAYFEAAQKRGLRMMGGKVLQD
                     RHSPDGLRDETEQSLLDSEALIRRWHGKDRLGYAITPRFAPTSSAAQLRGAGELAARY
                     RDTWIHSHVAENRDEVRWVRELYPQARSYLDVYRSFGLLRERAVYAHCIHLDEPDRQL
                     LRDSGAAAAVSPTSNLFLGSGFFDFAGAQRVGFLHGLASDVGGGTSFSPFHTMLAAYF
                     VGREGETKQGLSLQPSHLWWLHTGGAARALGLEGVIGTLQAGTEADFVLLNPRATPLL
                     ARKTERAQNLEELLFALIVLGDDRVVEQTVIA"
     gene            complement(1278..>1951)
                     /locus_tag="PK242_14020"
     CDS             complement(1278..>1951)
                     /locus_tag="PK242_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOZ95184.1"
                     /translation="GGNEFRGIFSAPPSIEPTFLKLDIMTALTKGLLNVVLVFFLVEL
                     FDATGTLMGVARRAGLLVPGRMDRLNKSLFADSAAIFAGSLLGTSSTTAYVESASGVQ
                     AGGRTGLTALTVSVLFLAALFIAPLAGAVPAYATAPALFFVGCLMMRDLTELEWEDST
                     EVIPAAVTALMMPFTYSIANGLAFGFITYAAIKLLTGRVREVHWMVWLIAAVFLFKFF
                     YIPGG"
BASE COUNT          330 a          661 c          683 g          277 t
ORIGIN      
        1 gcaatcaccg tctgctcgac cacccggtcg tcgcccagca cgatcagggc gaacagcagc
       61 tcctccaggt tctgcgcgcg ctcggtcttg cgcgccagca gcggcgtggc cctggggttg
      121 agcagcacga aatcggcctc ggtgccggcc tgcagggtgc cgatcacgcc ctccagaccc
      181 agtgcacgcg ccgcgccgcc ggtgtgcagc caccacaggt gcgacggttg caggctcagg
      241 ccctgcttgg tctctccttc gcgcccgacg aaataggccg ccagcatggt gtggaacggg
      301 ctgaagctgg tgccaccgcc cacgtcgctg gccagaccgt gcagaaaccc gacccgctgc
      361 gcccctgcga agtcaaagaa cccgctgccc agaaacaggt tgctggtggg gctgacggcg
      421 gcggcggcgc cgctgtcgcg cagcaactgg cggtcgggtt cgtccaggtg gatgcagtgc
      481 gcgtacaccg cgcgctcgcg cagcaggccg aagctgcggt acacgtccag gtacgagcgc
      541 gcctgcgggt acagctcgcg cacccacctg acctcgtcgc ggttttcggc cacgtgggaa
      601 tggatccagg tgtcgcggta gcgcgccgcc agctcgcccg cgccgcgcag ctgcgccgcg
      661 ctgctggtgg gggcaaagcg tggcgtgatg gcatagccca gccggtcctt gccgtgccag
      721 cggcggatca gcgcctcgct gtcgagcagg ctctgctcgg tctcgtcgcg caggccgtcg
      781 ggcgagtggc ggtcctgcag caccttgccg cccatcatgc gcaggccgcg tttttgtgca
      841 gcctcgaaat aggcgtccac cgacaccggg tgcgaggtgg caaaggtgag cgccgtggtc
      901 acgccgtggc gcagcagctc ggcaaagaac acgttcgcca tctcggccgc ataggccggc
      961 gaatcgaagc gggcctcggc cgggaaggtg tagttctcca gccagggcag caggccgtcg
     1021 gccggcgagc cgatgatgtc ggtctgcggg aaatgcacgt gcatgtcgat gaagcccggc
     1081 gcgatcaggc gaccgggcca gtgtgtcacc ggcacctgcg ggtggcgcgc cgccaccgac
     1141 cggtaggggc cggcgtcgat gacgcgctgc acgccatcga cgcccaggcc caccaccagc
     1201 agcccgtcgc tgtcgtgcag cggggcgccg tgggggtcga aacgcagcag ctctgcccgg
     1261 tacgcgttca cggcgactca acctcccgga atgtagaaga acttgaacag gaacacggca
     1321 gcgatcagcc agaccatcca gtgcacttcg cgcacccggc cggtcagcag cttgatggcg
     1381 gcgtaggtga tgaagccgaa ggccaggccg ttggcgatgg agtaggtgaa gggcatcatc
     1441 agcgccgtca ccgccgccgg gatcacttcg gtggaatctt cccattccag ctcggtcagg
     1501 tcacgcatca tcaggcagcc gacgaaaaac agcgccggcg ccgtggcgta ggctggcacc
     1561 gcaccggcca gcggtgcgat gaacagggcg gccaggaaca gcaccgacac cgtcagggcc
     1621 gtcaggccgg tgcgcccgcc ggcctgcacg cccgaggcgc tttcgacgta ggcggtggtg
     1681 ctggacgtgc ccagcagcga gccggcaaag atggcggcgc tgtcggcgaa cagcgatttg
     1741 ttgagccggt ccatgcggcc cggcaccagc aggccggcac gccgcgccac gcccatcagg
     1801 gtgccggtgg cgtcgaacag ttcgaccagg aagaacacca gcaccacgtt cagcagaccc
     1861 ttggtcagcg cggtcatgat gtcgagcttc agaaatgtcg gctcgatcga gggcggcgcc
     1921 gagaagatgc cgcggaactc gttgccgccg a
//
LOCUS       DAOOOG010000329         1949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_74044_length_1949_cov_24.535375, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000329 DAOOOG010000000
VERSION     DAOOOG010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74044_length_1949_cov_24.535375"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /gene="eno"
                     /locus_tag="PK242_14025"
     CDS             <1..443
                     /gene="eno"
                     /locus_tag="PK242_14025"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043750.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOZ95185.1"
                     /translation="ISIEDGMAEGDWDGWKLLTERLGDKVQLVGDDLFVTNTKILKEG
                     IDKGIANSILIKINQIGTLTETFDAIEMAKRAGYTAVISHRSGETEDSTIADIAVGLN
                     AGQIKTGSLSRSDRMAKYNQLLRIEEDLGDMARYPGRAAFYNLK"
     gene            565..843
                     /locus_tag="PK242_14030"
     CDS             565..843
                     /locus_tag="PK242_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOZ95186.1"
                     /translation="MTSRVVPAILLLLLVVIHAQLWVGRGSLPNVSALQRKLDQQRSA
                     NQQARLVNEQLASEVEDLRAGLGMVEERARIELGMVKPNEIFVQVAPR"
     gene            complement(875..1441)
                     /gene="rimM"
                     /locus_tag="PK242_14035"
     CDS             complement(875..1441)
                     /gene="rimM"
                     /locus_tag="PK242_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574720.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOZ95187.1"
                     /translation="MVPGLEPAELPADAVEVGRILDAWGIQGWFKVQPYSASPEALFS
                     CKRWWLLPPERAPKPAWPGAVALNIRQARAHTDAVVASAADITDRDAALGLKGARIFI
                     PRSAFPAPAEGEYYWVDLIGLTVVNREGLVLGQVSDLLATGPQQVLVVGHQEAGKARE
                     RLIPFVDAYVDEVDLPARQIRVDWQPDY"
     gene            complement(1456..1716)
                     /gene="rpsP"
                     /locus_tag="PK242_14040"
     CDS             complement(1456..1716)
                     /gene="rpsP"
                     /locus_tag="PK242_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOZ95188.1"
                     /translation="MVVIRLSRGGAKARPFYSIVVADKRARRDGSFIERIGFYNPGAA
                     QGEEGLRIAQDRLTHWLGVGAQPSLTVQRLIKQAAKAAVAAA"
BASE COUNT          334 a          676 c          665 g          274 t
ORIGIN      
        1 tcatcagcat cgaggacggc atggccgagg gcgactggga cggctggaag ctgctcaccg
       61 agcgcctggg cgacaaggtg cagctggtgg gcgacgacct gttcgtcacc aacaccaaaa
      121 tcctcaagga aggcatcgac aagggcatcg ccaactcgat cctcatcaag atcaaccaga
      181 tcggcaccct gaccgagact ttcgacgcca tcgagatggc caagcgcgcc ggctacaccg
      241 ccgtcatcag ccaccgctcg ggcgagaccg aggactccac catcgccgac atcgccgtcg
      301 gcctgaacgc cggccagatc aagaccggct cgctgtcgcg ctcggaccgc atggccaagt
      361 acaaccagct gctgcgcatc gaagaggatc tgggcgacat ggccaggtac cccgggcgcg
      421 ccgctttcta caacctgaag tgatgcggcg cgctgcggca cgtcggcggg tcggcatggc
      481 ctgcgtcctg gcagcgtgcc ggcgcggttc ggagctatac taaaaagata gcaacgaatc
      541 cgcttgcacc ctgcgcctgc tgccatgacc tcccgcgtcg tcccggccat cctgctgctc
      601 ctgctggtcg tcatccacgc ccagctgtgg gttgggcgcg gcagcctgcc caacgtgtcg
      661 gcgctgcaac gcaagctgga tcagcaacgc agcgccaacc agcaggcgcg gctggtcaac
      721 gagcagctgg cgtccgaggt cgaagacctg cgcgccgggc tgggcatggt cgaggaacgc
      781 gcccgcatcg agctgggcat ggtcaagccc aacgaaatct tcgtgcaggt ggcgccgcgc
      841 tgacgatgag cgcctgcggc cagcgtggcc aggttcagta gtcgggctgc caatcgaccc
      901 tgatctgccg ggccggcagg tcgacctcgt cgacataggc gtcgacgaac gggatcaggc
      961 gctcacgcgc cttgccggcc tcctggtgac cgaccaccag cacctgctgc gggccggtgg
     1021 ccagcaggtc gctcacctgc cccagcacca ggccctcacg gttgaccacg gtcaggccga
     1081 tcagatcgac ccagtagtac tcgccttcgg ccggtgccgg aaacgccgaa cgcggaatga
     1141 agatgcgtgc gcccttgagg cccagggcag cgtcgcggtc ggtgatgtcg gccgcgctgg
     1201 cgaccacggc atcggtgtgg gcgcgcgcct ggcggatgtt cagcgccacg gcgccgggcc
     1261 aggcgggctt gggcgcgcgc tcgggtggca gcagccacca gcgtttgcaa gaaaaaagcg
     1321 cctcgggcga ggcgctgtag ggctgaacct tgaaccagcc ctggatgccc caggcgtcca
     1381 ggatgcggcc gacttcgacc gcgtccgccg gcagctcggc cggctccagc cccgggacca
     1441 tgcgggcggg cgcgctcagg cggcggccac agcggccttg gcggcctgct tgatcaggcg
     1501 ctgcaccgtg agcgacggct gcgcgccgac gcccagccag tgcgtcaggc gatcctgggc
     1561 gatgcgcagg ccctcttcac cctgggcggc gccggggttg tagaaaccga tgcgctcgat
     1621 gaagctgccg tcgcggcgcg cgcgcttgtc ggcaaccacg atgctgtaga agggacgggc
     1681 cttggcgccg ccgcgcgaga gacgaataac gaccatgttg aactgaaatc ctgtgggtgg
     1741 tggctcgggc ctcggcgctt caggttgggg caaaagcgcg caaggcccag gttcttccgg
     1801 tgctgagaca cgcgacatgg ccacccggcc agcgacacac cgaagagcct atgattatag
     1861 cggctgccgc cgctgcgccc tgccccccga ggccggccgc cgccggcggc gcccagaaac
     1921 cccggcccgc acggcgcggg cgcccgtcc
//
LOCUS       DAOOOG010000330         1922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_75620_length_1922_cov_38.465453, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000330 DAOOOG010000000
VERSION     DAOOOG010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75620_length_1922_cov_38.465453"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PK242_14045"
     CDS             complement(<1..971)
                     /locus_tag="PK242_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOZ95189.1"
                     /translation="MNTPALQRAVFLLLLAGVTLAFFWIIAPFFGAVFWAVVLTLLFM
                     PLFRRLRAALRGRATLAALATMLICVLIVVVPLGFIVGAMVDEAASFTQRVRSGELNL
                     PAYFQQVLDALPAWLRGLLGRFGLLSMQDVGAKLAAALVQGGQAITGHALAIGQNTLL
                     LLVNLGIMLYLLFFFLRDGRELALLMRRAVPMQPAHASYLLHKFATVVRATVKGTVVV
                     ALVQGLLGGLAFAVLGIHGAVLWGVTMAVLSLVPAVGAALIWAPVAIYLLATGSPGAG
                     IGLAAWGVGVMGMVDNLLRPLLVGKETKLPDFLVLLSTIGGISVFGV"
     gene            1103..1525
                     /locus_tag="PK242_14050"
     CDS             1103..1525
                     /locus_tag="PK242_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HOZ95190.1"
                     /translation="MSATLKPPPESLTVHTLSRMLELRWPDGAQFRIPFELLRVYSPS
                     AEVQGHGPGQEVLQTGKREVEITALAPVGNYAVQPRFSDGHESGIYTWAYLYELGERQ
                     DALWRQYLDRLAAAGLDRDAPMGGKAGGAVSITKTRAS"
     gene            complement(1730..>1922)
                     /locus_tag="PK242_14055"
     CDS             complement(1730..>1922)
                     /locus_tag="PK242_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyvinyl-carboxyphosphonate
                     phosphorylmutase"
                     /protein_id="HOZ95191.1"
                     /translation="LGYGLVLYANAALQGALAGMQQALTALRDTGQVLESSGLVTPFA
                     ERQRLVGKPRWDALEQRYR"
BASE COUNT          335 a          703 c          652 g          232 t
ORIGIN      
        1 ttgacgccga acaccgaaat gccgccgatg gtcgacagca gcaccaggaa gtccggcagc
       61 ttggtttcct tgcccaccag cagcgggcgc agcaggttgt cgaccatgcc catcacgccc
      121 acgccccagg ccgccaggcc gatgccggcg ccgggcgaac cggtggccag caggtagatc
      181 gccaccggcg cccagatcag cgccgcgccc accgccggca ccagcgacag caccgccatg
      241 gtgacgcccc acagcacggc gccgtggatg cccagtaccg caaacgccag cccgcccagc
      301 aggccctgca ccagcgccac caccaccgtg cccttgacgg tggcgcgcac cacggtggcg
      361 aacttgtgca gcaggtagct ggcgtgcgcc ggctgcatcg gcacggcgcg gcgcatcagc
      421 agcgccagct cgcgcccgtc gcgcaggaaa aagaacagca ggtacagcat gatgcccagg
      481 ttgaccagca gcagcagcgt gttctgcccg atcgccagcg cgtggccggt gatggcctgg
      541 ccgccctgca ccagcgcggc cgccagcttg gcgcccacgt cctgcatgct gagcagcccg
      601 aagcggccca gcagcccgcg cagccaggcc ggcagtgcgt ccagcacctg ctggaaatag
      661 gccggcaggt tgagctcgcc cgaacgcacg cgctgggtga agctggccgc ctcgtccacc
      721 atggcgccga cgatgaagcc cagcggcacc accacgatca gcacgcagat cagcatggtg
      781 gccagcgccg ccagcgtggc gcgcccgcgc agggcggcgc gcaggcggcg aaacagcggc
      841 atgaacagca gcgtcagcac cacggcccag aacaccgcac cgaagaacgg cgcgatgatc
      901 cagaaaaacg ccagcgtcac cccggccagc agcagcagaa agacggcgcg ttgcaaggcg
      961 ggggtgttca cggcgggtgg gtggcggggt gggcggtgcg cgcagtatag gagccgacgc
     1021 cggacggcgg ccgtgggcgc acaatcgacg cacaatcgct gccgtcgcag ccgaacacgc
     1081 gccgccccgg cgtgcacctg ccatgtccgc cacgctcaaa ccgccgcccg aatcgctcac
     1141 cgtgcacacc ttgtcgcgca tgctggagct gcgctggccc gacggcgcgc agttccgcat
     1201 cccgttcgag ctgctgcgcg tgtactcgcc ctcggccgag gtgcagggcc atggcccggg
     1261 ccaggaagtg ctgcagaccg gcaagcgcga ggtcgagatc accgcgctgg ccccggtcgg
     1321 caactacgcc gtgcagccac gcttttcgga cgggcacgag agcggcatct acacctgggc
     1381 ctacctgtac gaactgggcg aacgccagga cgcgctgtgg cgccagtacc tcgatcgact
     1441 ggccgccgcc ggcctggacc gcgacgcccc catgggcggc aaggcgggcg gcgcggtgtc
     1501 gatcaccaag accagggcgt cctgactgcc gacccaggcg ttcgcggggc ggccagaccc
     1561 gccgcgggcg cgcatgggcg ccagtggctg attcctgtca tcgccgcttt cgcagggatg
     1621 acggtgggtt tgctcggaca tcgcgagccc gtgacagaca aggcatgtgc tatcaattaa
     1681 atagcttctg gcgctcggct gacgccaggt gaaggccatt tggccattgt cagcggtagc
     1741 gctgctccag cgcgtcccag cgcggcttgc ccaccaggcg ctggcgctcg gcaaacggcg
     1801 tcaccaggcc gctggattcg agcacctggc cggtgtcacg cagcgccgtc agcgcctgct
     1861 gcatgccggc cagcgcgccc tgcagcgccg cgttggcgta cagcaccagg ccgtagccga
     1921 gc
//
LOCUS       DAOOOG010000331         1919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_75785_length_1919_cov_26.143777, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000331 DAOOOG010000000
VERSION     DAOOOG010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75785_length_1919_cov_26.143777"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..702)
                     /locus_tag="PK242_14060"
     CDS             complement(238..702)
                     /locus_tag="PK242_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95192.1"
                     /translation="MRAIELFHLRRVRDKPRALALIAAHARLSGDQALAVLHAAIGGG
                     RPRLCLSDDEAARACIVALAPAGFVARFAPGADFDLAQHAQQALMAALPACAPDLAAQ
                     AGARLLHDDWPEALALALQHLRMHRPAQHPGRRRLEQAAIDTGLVRGVPGRT"
     gene            complement(721..1773)
                     /gene="murB"
                     /locus_tag="PK242_14065"
     CDS             complement(721..1773)
                     /gene="murB"
                     /locus_tag="PK242_14065"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOZ95193.1"
                     /translation="MFVERNVSLQPLNSFGIAARAAHMVRVRSEDEVRAVLADAGLTA
                     AGLFVLGGGSNIVLTGDLRALVLKVEVPGRRVVDQTARHTIVEAGAGESWHDFVGWTL
                     DQGLPGLENLALIPGTVGASPVQNIGAYGVELQDRFHELDAIDLATGELFTLNAAQCG
                     FGYRDSVFKHAPAQPGDFGLARRALILRVRLALPRAWRPVLGYLDLERRMQETGISAP
                     DARQIHDWIVAIRRAKLPDPAVIGNAGSFFKNPTVTPEQCQDIIGRDPKLVHYPMPDG
                     SVKLAAGWLIDACGWKGKRMGNAAVYDKQALVLVNQGGSQHPATGGEVMTLARAIQTS
                     VYERFGIRLEPEPVVV"
     gene            1817..>1919
                     /locus_tag="PK242_14070"
     CDS             1817..>1919
                     /locus_tag="PK242_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HOZ95194.1"
                     /translation="MPSFDTVCDPDMVEVRNAVDNAAKEIGTRFDFKG"
BASE COUNT          296 a          681 c          687 g          255 t
ORIGIN      
        1 cagcagcacc acggcagcaa agacggccgc gcgccaggcc ttgcggcgat aggccagtgc
       61 ctcgtcctgc atgccctcca tgcggccgat ggccaccgca cggatcaggg ccatgacgag
      121 gtacagcagc gcgccgatca acagcacggc cagcacggtg gaaaaacgca tggggcaatc
      181 tgggagggtc gggatggcgc gatgatagtc agccgccccg ccgcccaggt gcgccgctca
      241 ggtgcgcccc ggcaccccgc gcaccaggcc ggtgtcgatg gcggcctgct ccagccgccg
      301 tcgacccggg tgctgcgccg gacggtgcat gcgcaggtgc tgcagggcca gcgccagcgc
      361 ctcgggccag tcgtcgtgca gcagccgggc gccagcctgt gcggccaggt cgggggcgca
      421 ggcgggcagc gccgccatca gggcctgctg ggcatgctgg gcgaggtcga agtcggcgcc
      481 gggggcgaaa cgcgccacga acccggcggg cgccagcgcc acgatgcagg cgcgcgccgc
      541 ctcatcgtcc gacagacaca ggcgcggccg gccgccgccg atggctgcgt gcagcacggc
      601 cagcgcctga tcgcccgaca gcctggcgtg cgccgcgatc agcgccaggg cgcgcggctt
      661 gtcgcgcaca cgccgcaggt ggaacagctc gatggcgcgc atgggcgcag tcggtggcgc
      721 tcaaaccacc accggctcgg gctccaggcg gatgccgaag cgctcgtaca cgctggtctg
      781 gatggcgcgc gccagggtca tcacctcacc gccggtggcc ggatgctggc tgccgccctg
      841 gttgaccagc accagcgcct gcttgtcgta gacggcggcg ttgcccatgc gcttgccctt
      901 ccagccgcag gcgtcgatca gccaccccgc cgccagcttg acgctgccgt cgggcatcgg
      961 gtagtgcacc agcttggggt cgcgaccgat gatgtcctgg cactgctcgg gcgtcacggt
     1021 cgggttcttg aaaaagctgc cggcgttgcc gatgaccgcc gggtcgggca gcttggcgcg
     1081 gcggatggcg acgatccagt cgtggatctg gcgcgcatcg ggcgcgctga tgccggtttc
     1141 ctgcatgcgg cgttccaggt ccaggtagcc cagcaccggc cgccaggcgc gcggcagcgc
     1201 cagacgcacg cgcaggatca gcgcgcgcct ggccagcccg aagtcgcccg gctgcgccgg
     1261 cgcgtgcttg aacaccgaat cgcgatagcc gaagccgcac tgagcggcgt tcagggtgaa
     1321 cagctcgccg gtggccagat cgatggcgtc gagctcgtga aagcggtcct gcaactcgac
     1381 cccgtaggcg ccaatgttct gcaccgggct ggcgcccacg gtgccgggga tcagcgccag
     1441 gttttccagg ccgggcaggc cctggtccag cgtccagccg acgaagtcgt gccaggactc
     1501 gcccgcgccg gcctcgacga tggtgtggcg cgcggtctgg tccaccacgc ggcgacccgg
     1561 cacctcgacc ttcagcacca gcgcgcgcag atcgccggtc agcacgatgt tgctgccgcc
     1621 gcccagcacg aacaggccgg ccgccgtcag cccggcgtcg gccagcacgg cccgcacctc
     1681 gtcctcgctg cgcacgcgca ccatgtgggc ggcgcgcgcc gcgatgccga agctgttgag
     1741 gggctgcagg gagacgttgc gctcgacgaa catgggacaa ttgtcgcatc cggtatctgc
     1801 atgcaaaggt aaggccatgc cctctttcga cacggtttgc gaccccgaca tggtggaagt
     1861 caggaacgcg gtggacaacg ccgccaagga aatcggcacg cgcttcgact tcaagggca
//
LOCUS       DAOOOG010000332         1917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_75917_length_1917_cov_37.218582, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000332 DAOOOG010000000
VERSION     DAOOOG010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75917_length_1917_cov_37.218582"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PK242_14075"
     CDS             complement(<1..889)
                     /locus_tag="PK242_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ95195.1"
                     /translation="MKLDFDYQPLLLVPVLALLALPLVGSGSTWLTLTVAGLAMGMII
                     FIIASGLTLVFGLMDVLNFGHGVFIALGAFVATSVLGAMGDWTGSSDIWRNLVAVLPA
                     MLVAMLVAGAIGLAFERFIVRPVYGQHLKQILITMGGMIIGEELIKVIWGPQQIPLPL
                     PTGMQGSWLIGEAAVEKYRVVAVIVGLLVFALLAWTLSRTKVGLLIRAGVQDREMVES
                     LGYRIRRLFVGVFVAGSALAGLGGVMWGLYQQTVIPQMGAQVNILIFIVIIIGGLGST
                     TGALIGALLVGLMANYTGFL"
     gene            complement(967..1692)
                     /locus_tag="PK242_14080"
     CDS             complement(967..1692)
                     /locus_tag="PK242_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ95196.1"
                     /translation="MSETLLTLEGVHTHIGAYHILHGVDLQVPRGQLTMLLGRNGAGK
                     TTTLRTIMGLWQASAGRIGFNGQDITRRHTPQIAQLGIAYVPENMGIFADLTVKENMV
                     LAARGARSAAQIDGERLAWIFRLFPAVEKFWHHPAGKLSGGQKQMLAVSRAIVEPREL
                     LIVDEPSKGLAPAIINNMIDAFQQLKASGVTILLVEQNLSFARRLGDRVAVMDNGRIV
                     HAGSMQALAEDAALQQRLLGLSL"
     gene            complement(1689..>1917)
                     /locus_tag="PK242_14085"
     CDS             complement(1689..>1917)
                     /locus_tag="PK242_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ95197.1"
                     /translation="VEHKMDVVRELADRIIVLTNGQLVADGEPAEVIASPVVQEAYLG
                     LAPQGAFGSTDARPHPGLPPSGGRSLGDTAP"
BASE COUNT          326 a          671 c          624 g          296 t
ORIGIN      
        1 ccagaaagcc ggtgtagttg gccatcaggc cgaccagcag cgcgccgatc agcgctccgg
       61 tggtgctgcc caggccgccg atgatgatga cgatgaagat caggatgttg acctgcgcgc
      121 ccatctgcgg gatcacggtc tgctggtaca gcccccacat cacgccgccc agcccggcca
      181 gcgcgctgcc ggccacgaac accccgacaa agaggcgccg gatgcggtag ccgagcgact
      241 cgaccatttc cctgtcctgc actccggcgc gaatcagcag ccccaccttg gtgcgcgaca
      301 gcgtccaggc cagcagggcg aacacgagca ggccgacgat cacggccacc acgcggtatt
      361 tctcgacggc ggcttcgccg atcagccacg agccctgcat gccggtgggc agcggcagcg
      421 ggatctgctg cgggccccag atgaccttga tcagctcctc gccgatgatc atgccgccca
      481 tggtgatcag gatctgcttg agatgctggc catacaccgg gcgcacgatg aagcgctcga
      541 acgccaggcc gatggcgccg gccaccagca tggccaccag catggccggc agcaccgcca
      601 ccaggttgcg ccagatgtcg ctcgatccgg tccagtcgcc catggcgccc agcacgctgg
      661 tggcgacgaa ggcgcccagg gcgatgaaca cgccgtggcc gaagttcagc acgtccatca
      721 ggccgaacac cagcgtcagg cccgaggcga tgatgaagat gatcatgccc atggccagcc
      781 cggccacggt gagcgtcagc caggtcgagc ccgagcccac cagcggcagc gccagcagcg
      841 ccagcaccgg caccagcagc agcggctggt aatcgaagtc gagtttcatg cgcctcggag
      901 actacaaaaa caatagctgc tggcgcgcgt ccagcaagcg ccacaggcca attgaatgcg
      961 tatttgtcac agcgacagcc ccagcagccg ctgctgcagc gcggcgtcct cggccagcgc
     1021 ctgcatgctg ccggcgtgca cgatgcggcc gttgtccatc accgccacgc ggtcgcccag
     1081 gcggcgcgcg aagctcaggt tctgctccac cagcaggatc gtcacgccgc tggccttgag
     1141 ctgctggaag gcgtcgatca tgttgttgat gatggccggc gccagcccct tgctgggttc
     1201 gtcgacgatc agcagctcgc gcggctcgac gatggcgcgg ctgaccgcca gcatctgctt
     1261 ttgcccgccc gacagcttgc cggccgggtg gtgccagaac ttctccaccg cgggaaacag
     1321 ccggaagatc caggccagcc gctcgccgtc gatctgcgcc gcgctgcgtg cgccgcgcgc
     1381 ggccagcacc atgttctcct tcaccgtcag gtcggcgaag atgcccatgt tctcgggcac
     1441 gtaggcgatg cccagctgtg cgatctgcgg cgtgtgcctg cgggtgatgt cctggccgtt
     1501 gaagccgatg cgcccggcgc tggcctgcca caggcccatg atggtgcgca gtgtggtggt
     1561 cttgccggcg ccattgcgtc ccagcagcat ggtcagctgc ccgcgcggca cctgcagatc
     1621 caccccatgc aggatgtggt aggcgccgat gtgggtgtgc acgccttcga gcgtgagcag
     1681 ggtttcactc atggtgctgt atctcccaaa ctccttcccc cggatggggg aaggccggga
     1741 tgggggcgag cgtcggtgct gccgaaggca ccctggggcg ccagccccag gtaagcctcc
     1801 tgcaccaccg gcgaggcgat gacctcggcc ggctcgccgt cggccaccag ctggccgttg
     1861 gtcagcacga tgatgcggtc ggccagctcg cgcaccacgt ccatcttgtg ctcgacc
//
LOCUS       DAOOOG010000333         1901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_76837_length_1901_cov_35.132178, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000333 DAOOOG010000000
VERSION     DAOOOG010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76837_length_1901_cov_35.132178"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="PK242_14090"
     CDS             complement(<1..1238)
                     /locus_tag="PK242_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOZ95198.1"
                     /translation="MNQTLSPTLLTTLVFSLALLAYVAVKFWLASRQMRAVAAHRAAV
                     PTEFAGTIGLAAHQRAADYTIAKLRFGLLELLLSAAVLLGWTLLGGLDALNGALLDAI
                     GARPLAQPLALLVAFAFINAAIELPGTLYHTFVIEQRFGFNKMTRRLWLIDLAKTTLV
                     SSVIGVPLAALALWLMQATGALWWLWTWGAWMGFSLLMLVIYPTVIAPLFNRFEPLRD
                     DALSERVSQLMGRCGFAAKGLFVMDGSRRSAHANAYFTGFGAAKRVVFFDTLLQQLSP
                     PEVEAVLAHELGHFSHRHIVKRIVLLFAVSLAGLALLGWLARQPWFYGGLGVTPHLPP
                     ALGGAVPNHALALLLFMLVVPVFTFFLSPLMAALSRRHEFEADAYAVRQASAQDLGNA
                     LVKLYKDNASTLTPDPLYVR"
     gene            1349..>1901
                     /gene="orn"
                     /locus_tag="PK242_14095"
     CDS             1349..>1901
                     /gene="orn"
                     /locus_tag="PK242_14095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HOZ95199.1"
                     /translation="MNDTACTPEAGAALAKSELNLVWLDCEMTGLDPEKERLIEIAVI
                     VTGPQLTPRVEGPVLVIHQPDSVLDAMDAWNKGTHGRTGLIDKVRAATLSEAEAEARL
                     IDFLARYVPKGVSPLCGNSIGQDRRFLVRYMPRLEAFFHYRNVDVSTLKELARRWRPE
                     LVEAFKKQQSHTALADVHESIDEL"
BASE COUNT          331 a          638 c          691 g          241 t
ORIGIN      
        1 aagcgcacgt acagcgggtc gggcgtcagc gtgctggcgt tgtccttgta gagcttgacc
       61 agggcgttgc ccaggtcctg ggcgctggcc tgccggacgg cgtaggcgtc ggcctcgaac
      121 tcgtgccggc gcgacaaggc cgccatcagc ggcgacagga agaaggtgaa caccggcacc
      181 accagcatga acagcagcag cgccagcgca tggttgggca ccgcgccccc cagggccggc
      241 ggcaggtggg gcgtcacccc cagaccgccg tagaaccagg gttgtcgcgc cagccagccc
      301 agcagcgcca ggccggccag gctgaccgcg aacagcagga cgatgcgctt gacgatgtgg
      361 cggtggctga agtggcccag ctcgtgcgcc agcacggcct cgacctcggg cgggctgagc
      421 tgctgcagca gggtgtcgaa gaacaccacc cgcttggcgg cgccgaaacc ggtgaaatag
      481 gcgttggcgt gcgcgctgcg gcggctgccg tccatcacga acaggccctt ggcggcaaag
      541 ccgcagcgcc ccatcagctg gctgacgcgc tcgctcagcg cgtcatcgcg cagcggctcg
      601 aagcggttga acagcggcgc gatgacggtg gggtagatca ccagcatcag caggctgaag
      661 cccatccacg cgccccaggt ccacagccac cacagcgcgc ccgtggcctg catcagccac
      721 agggccaggg ccgccagcgg cacgccgatg acgctgctga ccagcgtggt cttggccagg
      781 tcgatcagcc acaggcggcg cgtcatcttg ttgaagccaa agcgctgctc gatcacgaag
      841 gtgtggtaga gcgtgcccgg cagttcgatg gcggcgttga tgaaggcgaa ggccaccagc
      901 agcgccagcg gctgcgccag cgggcgcgcg ccgatggcgt ccagcagcgc gccgttgagc
      961 gcgtccagcc cgcccagcag cgtccagccc agcagcacgg cggccgacaa cagcagctcc
     1021 agcaggccga agcgcagctt ggcgatggtg tagtcggccg cgcgctggtg cgccgccagc
     1081 ccgatggtgc cggcgaactc ggtcggcacg gccgcgcgat gggccgccac ggcgcgcatc
     1141 tggcgcgagg ccagccagaa cttgacggcc acgtaggcca gcagcgccag gctgaagacc
     1201 agggtggtca ggagggtggg ggagagcgtt tggttcaaga cggactcggc agtaagggga
     1261 ggcgccaagc cgccaagttt agggtgctcc ggccggctgc aagggcgggc ttgcgacgcc
     1321 ccgccggcgg ccgtggacaa tcggcgccat gaacgacacc gcttgcaccc ccgaagccgg
     1381 cgccgcgctg gccaaatccg agctgaacct ggtctggctc gactgcgaga tgaccgggct
     1441 ggaccccgag aaggagcgcc tgatcgagat cgccgtcatc gtcaccgggc cgcagctgac
     1501 gccacgcgtc gaaggcccgg tgctggtgat tcaccagccc gacagcgtgc tggacgccat
     1561 ggacgcctgg aacaagggca cgcatgggcg caccggcctg atcgacaagg tccgggccgc
     1621 cacgttgagc gaggccgagg ccgaggcgcg gctgatcgac tttctggcgc gctacgtgcc
     1681 caagggcgtg tcgccgctgt gcggcaactc gatcgggcag gaccggcgct ttctggtgcg
     1741 ctacatgccg cggctggagg cgttcttcca ctaccgcaac gtcgacgtca gcacgctcaa
     1801 ggagctggcg cggcgctggc ggccggagct ggtggaggcc ttcaagaagc agcagtcgca
     1861 caccgccctg gccgacgtgc acgagtcgat cgacgagctg g
//
LOCUS       DAOOOG010000334         1895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_77217_length_1895_cov_20.259783, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000334 DAOOOG010000000
VERSION     DAOOOG010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77217_length_1895_cov_20.259783"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PK242_14100"
     CDS             complement(<1..196)
                     /locus_tag="PK242_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481775.1"
                     /note="with TatABE forms the twin-arginine translocation
                     complex which is involved in the transport of proteins
                     across the cytoplasmic membrane; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HOZ95200.1"
                     /translation="MSNPTPDPEDELAGTEQPFVVHLMELRDRLIKSMVAVAVVAGAL
                     ALYPGPGALYDLLAAPLVAHL"
     gene            complement(313..792)
                     /gene="tatB"
                     /locus_tag="PK242_14105"
     CDS             complement(313..792)
                     /gene="tatB"
                     /locus_tag="PK242_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HOZ95201.1"
                     /translation="MLDLGISKMALIGAVALIVIGPEKLPRVARTVGTLLGKAQRYVA
                     DVKSEVNRAMDLDELKKMKESVESAGREVEQSIHATQSAFEKDWNEATAGLGGTGTGD
                     DAPIPSWEPAPPQYRRPDKNWRLKRGATPQWYKQRHGVRRQVQSGAARVARYRPKSP"
     gene            complement(867..1097)
                     /gene="tatA"
                     /locus_tag="PK242_14110"
     CDS             complement(867..1097)
                     /gene="tatA"
                     /locus_tag="PK242_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HOZ95202.1"
                     /translation="MGTFSIWHWLIVLLIVVMIFGTKKLKNIGSDLGGAVKGFKDGMK
                     DGAAEADAPPAGQVTNAAAAKDTIDVQAKEKS"
     gene            complement(1252..1617)
                     /locus_tag="PK242_14115"
     CDS             complement(1252..1617)
                     /locus_tag="PK242_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOZ95203.1"
                     /translation="MSAHADPHCIFCKIVAGQIPSRKVYEDDELFAFHDIHPWAPVHF
                     LMVPKLHVASMAHVGPEHEALLGRIMALAPKLALEQGCQPYPDGGFRIVVNTGAQGGQ
                     EVHHLHVHVMGGPRPWARG"
BASE COUNT          284 a          643 c          654 g          314 t
ORIGIN      
        1 gcaggtgcgc caccagcggc gccgccagca ggtcgtacag cgcgcccggc cccggataga
       61 gcgccagcgc ccccgccacc acagccaccg ccaccatcga cttgatcagc cgatcgcgca
      121 gctccatcag gtggacgacg aatggctgtt cggtgccggc cagttcgtct tcggggtcgg
      181 gcgtggggtt ggacatggtg gtaggggtgt catgacgagc gactgcgccg ccgggggcgg
      241 cttcaacgat gcatggccga tgccggcctg gcgtcatcga tgccgcgctg cggcagggtc
      301 aacggtcatg ggtcatggac tcttggggcg atagcgcgcc acgcgcgccg cgcccgactg
      361 cacctgccgg cgcacgccgt ggcgctgctt gtaccactgc ggcgtggcgc cgcgcttgag
      421 gcgccagttc ttgtcggggc gtcggtattg cggcggcgcc ggctcccagg acgggatcgg
      481 ggcgtcgtcg cccgtgccgg taccgcccag tccggcggtg gcctcgttcc agtccttctc
      541 gaacgccgac tgggtggcat ggatgctctg ctcgacttcg cggccggcgg attccaccga
      601 ctccttcatc ttcttgagct cgtcgaggtc catcgcacga ttgacctcgg acttgacgtc
      661 agccacgtag cgctgggcct tgcccagcaa cgtgcccacc gtgcgtgcca cgcgcggcaa
      721 cttttcgggg ccgatgacga tcagcgccac ggcgccgatc agcgccatct tggaaatacc
      781 gagatcgagc atctgaaggg ttcagcctcg cgggcagcca gcgtcgggcg tgtcggccac
      841 gccccgcgcg cacgcggcgg gccggctcag ctcttttcct tggcctgcac gtcgatggtg
      901 tccttggccg cggcggcgtt ggtcacctga ccggccggcg gcgcgtcggc ctcggcggcg
      961 ccgtccttca tgccgtcctt gaagcccttg acggcgccgc ccaggtcgga gccgatgttc
     1021 ttgagcttct tggtgccgaa gatcatgacc acgatcagca gcacgatcag ccagtgccag
     1081 atcgaaaaag tacccatgtt gcaactcctt gcgccgaccg atgaacgggc gcgaatcaga
     1141 atgattttag acgtccgggg atgggcccgg ttccagcgcg atcatgcccg ttgccggcca
     1201 cgaccggtac accccggcgc gggcttgcgg cgccactcgc cggcggccgc ttcacccgcg
     1261 cgcccacggg cgcggcccgc ccatcacgtg cacgtgcaga tgatgcactt cctggccacc
     1321 ctgagccccg gtattgacca cgatgcgaaa cccgccgtca gggtacggct ggcagccctg
     1381 ttccagcgcc agcttgggcg ccagcgccat gatgcggccc agcagcgctt cgtgctccgg
     1441 ccccacatgg gccatggagg cgacgtgcag cttgggcacc atcaggaagt gcaccggcgc
     1501 ccaggggtgg atgtcgtgaa aggcaaacag ctcgtcgtcc tcgtacacct tgcgcgatgg
     1561 aatctgtccg gcaacgatct tgcagaagat gcagtggggg tcggcgtgag cgctcatgga
     1621 agacatcggt gacagtgggt tggcaatggc agaacccccg gcggtgcgac ggcgcgggga
     1681 cacgccctga cggatgatga acggcccgtc tgcgcccgga tggccgtgcg cgctgcggtc
     1741 gtgccggagc cggtggccag ggtcatcaag tggtacgcca tgcgggttcg atgggcgtga
     1801 cacaggcgcg ccgttcgggc ggcgcgctta cgcctcgacc gggcgcccgg cgttcatgcg
     1861 caccatgccc agcacgatgc ggtagaggaa ccaga
//
LOCUS       DAOOOG010000335         1885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_77817_length_1885_cov_32.545355, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000335 DAOOOG010000000
VERSION     DAOOOG010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77817_length_1885_cov_32.545355"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="PK242_14120"
     CDS             <1..108
                     /locus_tag="PK242_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOZ95204.1"
                     /translation="LLGSHYADIFAFIVLIIVLTLRPSGLLGERVADRA"
     gene            128..1315
                     /locus_tag="PK242_14125"
     CDS             128..1315
                     /locus_tag="PK242_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ95205.1"
                     /translation="MTQKNKLIAFVLCGIALLVLPLILQQFGNAWVRIVNIALLYTML
                     ALGLNIVVGYAGLLDLGYVAFFAVGAYLFALLASPHLTDNFAAIAAMFPGGLHLSVWL
                     ALPLALLLAAIAGVLLGIPVLKLRGDYLAIVTLGFGEIIRIFLNNLDHPFNITNGPKG
                     VNQIDPVRFLGINLGKPLNIGGFEVASVSLYYYLFLVLVLVTVFVCQRLQDSRVGRAW
                     MAIREDEIAAKAMGLNVRNLKLLAFGMGASFGGVSGAMFGAFQGFVSPESFSLMESVM
                     IVAMVVLGGLGHLPGVVLGALLLSALPEVLRYVAGPLQQMTDGRLDAAILRQLFIALA
                     MIIIMLLRPRGLWPSPEHGKVKTEPADRITPLSGQQNPPGVDFPGDAVPGNTPHRQYP
                     VNP"
     gene            1338..>1885
                     /locus_tag="PK242_14130"
     CDS             1338..>1885
                     /locus_tag="PK242_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ95206.1"
                     /translation="MSTDTVLKVQGVSKRFGGLQALSDVGISIQRGQVYGLIGPNGAG
                     KTTFFNVLTGLYTPDSGSFELAGQAYKPTAVHEVAKAGIARTFQNIRLFAEMTALENV
                     MVGRHVRTHSGLFGAVFRTAGFKAEEAAIAARAQELLDYVGIGKYAQFRARTLSYGDQ
                     RRLEIARALATDPQLVALDEPAA"
BASE COUNT          270 a          648 c          624 g          343 t
ORIGIN      
        1 ctgctgggca gccactacgc cgacatcttt gccttcatcg tgctgatcat cgtgctgacg
       61 ctgcggccct cgggcctgct gggcgagcgc gtggcggacc gcgcctgacg cccgggagga
      121 ccttgccatg acgcagaaga acaagctgat cgccttcgtg ctgtgcggca tcgcgctgct
      181 ggtgctgccg ctgatcctgc agcagttcgg caacgcctgg gtgcgcatcg tcaacatcgc
      241 gctgctctac accatgctgg cgctggggct gaacatcgtg gtcggctacg ccggcctgct
      301 ggacctgggc tacgtcgcct tcttcgcggt gggggcgtac ctgttcgcct tgctggcctc
      361 gccgcacctg accgacaact tcgccgccat cgccgccatg ttcccgggcg gattgcatct
      421 gtcggtgtgg ctggcgctgc cactggcgct gctgctggcg gccatcgcgg gggtgctgct
      481 gggcattccg gtgctcaagc tgcgcggcga ctacctggcc atcgtcacgc tgggcttcgg
      541 cgagatcatc cgcatcttcc tgaacaacct cgaccacccc ttcaacatca ccaacggccc
      601 caagggcgtc aaccagatcg atccggtgcg gtttctcggc atcaacctgg gcaagccgct
      661 gaacatcggc ggcttcgaag tcgcctcggt gtcgctgtac tactacctgt tcctggtgct
      721 ggtgctggtc acggtgttcg tgtgccagcg cctgcaggat tcgcgcgtcg gccgcgcctg
      781 gatggcgatc cgcgaagacg agatcgccgc caaggccatg ggcctgaacg tgcgcaacct
      841 caagctgctg gccttcggca tgggcgcctc gttcggcggc gtgtcgggag cgatgttcgg
      901 tgccttccag ggctttgtgt cgcccgagtc gttcagcctg atggagtcgg tgatgatcgt
      961 cgccatggtg gtgctgggcg gtctgggcca cctgcccggc gtggtgctgg gggcgctgct
     1021 gctgtcggcg ctgccggagg tgctgcgcta tgtggccggg ccgctgcagc agatgaccga
     1081 cggccggctg gacgcggcca tcctgcgcca gttgttcatc gcgctggcca tgatcatcat
     1141 catgctgctg cgtccgcgcg gcctgtggcc gtcgcccgag cacggcaagg tcaagaccga
     1201 gccggccgac cgcatcaccc cgctgtcggg gcagcagaac ccgccggggg tggacttccc
     1261 cggtgacgcc gtgcccggca acacgccgca ccggcagtac cccgtcaatc cctgaaaccc
     1321 aaccggagac gaacgtcatg agcacagaca ccgtcctgaa ggttcagggt gtttccaagc
     1381 gcttcggcgg cctgcaggcc ctgtccgacg tcggcatcag cattcagcgt ggccaggtgt
     1441 atggcctgat cggccccaac ggcgccggca agaccacctt cttcaatgtg ctgaccgggc
     1501 tgtacacgcc cgacagcggc agcttcgagc tggccggcca ggcctacaag ccgaccgctg
     1561 tgcatgaagt ggccaaggcc ggcatcgcgc gcaccttcca gaacatccgc ctgttcgccg
     1621 agatgacggc gctggaaaac gtgatggtcg ggcgccacgt gcgcacccat tccggcctgt
     1681 tcggcgccgt cttccgcacc gccggcttca aggccgagga ggcggccatc gccgcccgtg
     1741 cgcaggagct gctggactac gtgggcatcg gcaagtacgc ccagttccgc gcccgcacgc
     1801 tgtcttatgg cgatcagcgc cggctggaaa tcgcccgcgc gctggccacc gacccgcaac
     1861 tggtggcgct ggacgagccg gccgc
//
LOCUS       DAOOOG010000336         1882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_77971_length_1882_cov_29.513410, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000336 DAOOOG010000000
VERSION     DAOOOG010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77971_length_1882_cov_29.513410"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            399..983
                     /locus_tag="PK242_14135"
     CDS             399..983
                     /locus_tag="PK242_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOZ95207.1"
                     /translation="MRAAVRTLGLSALLLWAGTALAAQEIAGVKVEDAVTVAGTRLQL
                     NGSGVRYKGPFKVYVGDLYATRPVRSLDELIAAPGPKRLGMTFLREIQSAEFGKMLTR
                     GIEDNVSKTELSKIIPGMIKMGDIFAANKAFVPGDVCVLEWDPAKGLSIWAKGKLQAE
                     PMNDPAFYRALMSIWFGPTPADWKLKDALLGIKS"
     gene            complement(973..1695)
                     /gene="gph"
                     /locus_tag="PK242_14140"
     CDS             complement(973..1695)
                     /gene="gph"
                     /locus_tag="PK242_14140"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655418.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HOZ95208.1"
                     /translation="MSAPRGIDAAIIDLDGTMVDTLGDFVAALGRMLDDLGLPGVDAA
                     VVGRLVGKGSEHLLRSVLNHVLALQGQAPQATEIEALYPRAWARYQHHYLAINGRCAS
                     VYPGVVAGLTALRAAGLRLACLTNKPTAFALPLLRAKGLDGYFDQVFGGDAFARKKPD
                     PLPLLKTCEALGTPAARTLMVGDSSNDAAAARAAGCPVVLVTYGYNHGQPVREVDADG
                     FVDALHELLPTWLCGRPAGVRT"
BASE COUNT          301 a          625 c          665 g          291 t
ORIGIN      
        1 cggttgtagc cctggccggc caggctcttg aattccagtt cggtgatcac aggagactcc
       61 cagatcgggc ggcgtcggcg cgccgcgggt tcattcgggg gtggttggcc gcgcgacctg
      121 caacaggcgg ggcctcggaa agcaggccgg acaacccggc tgcggatcag gggttcagca
      181 gcgcaggctt gcgccagggc caggctccct ggcccccgtg acctgcgatg aacgcgctgg
      241 gaatgaacat gggcgcagtg tagcaaacgt gaacccggtc acggatcgcc gcgctggcac
      301 gtcaggggct ggggcaaacc ccgatgcagg ccatggccag ttcactaaac tttagtggtc
      361 aattcgaacg atcgtgcttt ttcaaaggag gttctcccat gcgcgctgcc gtacgcaccc
      421 ttggtctgtc tgccctgttg ctgtgggcgg gcaccgccct ggccgcgcag gagatcgccg
      481 gcgtcaaggt ggaagacgcc gtcaccgtgg ccggcaccag gctgcagctc aacggcagcg
      541 gcgttcgcta caaggggccg ttcaaggtct acgtgggtga cctgtacgcc acgcgcccgg
      601 tgcggtcgct ggacgagctg atcgccgcgc ccggccccaa gcgtctgggc atgacctttc
      661 tgcgcgagat ccagtcggcc gagttcggca agatgctcac acgcggcatc gaggacaacg
      721 tcagcaagac cgaactctcc aagatcatcc ccggcatgat caagatgggc gacatctttg
      781 ccgccaacaa ggccttcgtg ccgggcgacg tgtgtgtgct ggaatgggac ccagccaagg
      841 gcctgagcat ctgggccaag ggcaagctgc aggccgagcc gatgaacgat ccggccttct
      901 accgtgcgct gatgtcgatc tggttcggcc ccaccccggc cgactggaag ctcaaggacg
      961 cgctgctggg catcaagtcc tgacgccggc cggtcggccg cacaaccagg tcggcagcag
     1021 ctcgtgcagg gcgtcgacga agccgtcggc gtccacctcg cgcaccggct ggccgtggtt
     1081 gtagccatag gtgaccagca ccaccggaca gccggcggcg cgcgcggcgg cggcgtcgtt
     1141 gctggagtcg cccaccatca gggtgcgcgc cgcgggcgtg cccagggcct cgcaggtctt
     1201 gagcagcggc agcgggtcgg gcttcttgcg cgcgaaggcg tcgccgccga acacctggtc
     1261 gaaataaccg tccagaccct tggcgcgcag cagcggcagc gcaaaggcgg tgggcttgtt
     1321 ggtcaggcag gccaggcgca gaccggccgc tcgcagcgcc gtcagccccg ccaccacccc
     1381 cggatagacg ctggcgcagc ggccgttgat ggccaggtag tggtgctgat agcgcgccca
     1441 ggcccgggga tagagcgctt caatttctgt agcttgcggc gcttgtccct gcaacgccag
     1501 cacgtgattg agcacagaac gcagcaggtg ctcggagccc ttgccgacca gccgcccgac
     1561 caccgcggcg tcgacgccgg gcaggcccag gtcgtccagc atgcggccca gggcggcgac
     1621 gaaatcgccc agggtgtcga ccatggtgcc gtcgagatcg atgatggcgg cgtcgatgcc
     1681 gcgcggcgcg ctcactggac gaccctccgc acggcgtgct gcgggatgcg cgcttgggag
     1741 cggcccggcg cgctcattgg acgaccctcc gcacggcgtg ctgcgggatg cgcgcttggg
     1801 agcggcccgg cgcgctcatt aggtgtccct tcgcgctgac gcgctgcggg attcgcgctt
     1861 gggagcggcc cggcgcgctc at
//
LOCUS       DAOOOG010000337         1850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_80066_length_1850_cov_30.368245, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000337 DAOOOG010000000
VERSION     DAOOOG010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80066_length_1850_cov_30.368245"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..538)
                     /gene="crcB"
                     /locus_tag="PK242_14145"
     CDS             complement(152..538)
                     /gene="crcB"
                     /locus_tag="PK242_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOZ95209.1"
                     /translation="MHAVLAICLGACAGALLRWQLGLWLNHAGAWLPWGTLAANLIGG
                     YLVGLAVALLHHNPQIDATWRLLLVTGFLGALTTFSSFSAEVVEMALAQRWAQAAVTA
                     LAHLSGSLLLTFAGIRTALFFIAARA"
     gene            complement(574..1572)
                     /locus_tag="PK242_14150"
     CDS             complement(574..1572)
                     /locus_tag="PK242_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOZ95210.1"
                     /translation="MFKNLMLYRLGPGWPASAAQLEEALAREPFAECGATQQKSTGWV
                     APRGEQHGALVEAVDGQWIARFAIETKPVPGDAVRRKVQQAVAEIEKTSGRKPGKKEL
                     RDLRDDALLALLPQAFARRSQVTLWLNPQQRWLALDAGSQGKADEVIASLVRVAGQGF
                     AIHLLQTAHSPQAAMTGWLAATSADDWPEGLHVERECELKGSGDEPAVVKFTRHELAT
                     DEVRQHIAEGKLPTRLALGWQGRVGFVLTQALQLKKIGFLDGVFDERPAPASADERFD
                     ADVALATGELRGLITDLIAALGGEIEPGTPSGFTEAPTPASTVTEEATVAADDPPF"
BASE COUNT          285 a          648 c          634 g          283 t
ORIGIN      
        1 atcggttttc tttgcgtcct ctgcgaatcc ttggcgtcct ctgcgtatgg ctgttgattt
       61 ttctcatgcg tcgtgtcgca gtgcggcact gatcaagtac ctcaccatca ttcgcgagta
      121 gagcctcaaa tcggcttgca tcgcttgtcc atcaagcgcg agcagctatg aaaaacaaag
      181 cagtccggat gccggcgaag gtcagcagca gcgaacccga caggtgcgcc agcgccgtga
      241 ccgcggcctg ggcccagcgc tgcgccagcg ccatctcgac cacctcggcg ctgaagctgg
      301 aaaaagtggt gagcgcaccc agaaagcccg tcaccagcag cagccgccag gtggcgtcga
      361 tctgcgggtt gtggtgcagc agcgccaccg ccagcccgac caggtagccg ccgatcaggt
      421 tggccgccag cgtgccccag ggcagccagg cgccagcgtg gttgagccac aggccgagct
      481 gccagcgcag cagggcgccg gcgcaggcgc ccagacagat ggcgagaacg gcgtgcatcg
      541 gggttgcggg cgcttggccg gctcagcgcg tgctcagaac ggcgggtcgt ccgcggcgac
      601 cgtggcttct tcggtcacgg tgctggccgg tgtgggcgct tcggtgaagc ccgagggcgt
      661 gccgggttcg atctcgcctc ccagggcggc gatcaggtcg gtgatcagcc cgcgcagctc
      721 gccggtggcc agggccacgt cggcgtcaaa gcgttcgtcg gcgctggctg gcgccgggcg
      781 ctcgtcgaac acgccgtcca gaaagccgat cttcttcagt tgcagcgctt gcgtcagcac
      841 gaagccgacg cggccctgcc agccaagcgc cagccgcgtg ggcagcttgc cttcggcgat
      901 gtgctggcgc acctcgtcgg tggccagctc gtggcgggtg aacttgacca ccgccggctc
      961 gtcgccactg cccttgagct cgcactcgcg ctccacgtgc aggccctcgg gccagtcgtc
     1021 ggccgaggtg gcggccagcc agccggtcat ggcggcctgc ggcgagtgcg ccgtctgcag
     1081 caggtggatg gcaaagccct ggccggccac acgcaccagg ctggcaatca cttcgtcggc
     1141 cttgccctgg ctgccggcgt ccagcgccag ccagcgctgc tgcgggttca gccacagcgt
     1201 cacctggctg cggcgcgcaa aggcctgcgg cagcagggcc agcagggcgt cgtcgcgcag
     1261 gtcgcgcagc tccttcttgc cgggtttgcg accgctggtc ttttcgatct cagccaccgc
     1321 ctgctgcacc ttgcggcgca cggcgtcgcc cggcacgggc ttggtttcga tggcgaagcg
     1381 ggcgatccac tggccgtcca ccgcctcgac cagcgcgcca tgctgctcgc cgcgcggcgc
     1441 cacccagccg gtggatttct gctgcgtggc gccgcactcg gcaaacggct cgcgcgccag
     1501 cgcttcttcg agctgagccg ccgacgcggg ccagcccggg cccaggcggt agagcatcaa
     1561 attcttgaac acgaaattca acctgtcaca aacgccccgc gcggggccgg accggcatct
     1621 tagccacatc gacccggcag cagacggcgc cgcgccccca cggacccaat ggccgcggag
     1681 caggccaagc cagggcgccc gtggccgggg ttcccccggc cagtgggtgc gtcccccctc
     1741 cggggggaag gcgccgcagg cgacacaggg gggttacata ttcttcggca gccaggtggc
     1801 gatctgcggg aagaaccaca gcagcagcac catggcgcac atcaggaaga
//
LOCUS       DAOOOG010000338         1850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_80067_length_1850_cov_22.251811, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000338 DAOOOG010000000
VERSION     DAOOOG010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80067_length_1850_cov_22.251811"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK242_14155"
     CDS             <1..205
                     /locus_tag="PK242_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95211.1"
                     /translation="GGHSVIVIEHDLDLIAEADWIIDLGPEGGDGGGRIVAVGTPEDV
                     VRAGTHTGVALGAVLGRTPMAPG"
     gene            complement(323..1099)
                     /locus_tag="PK242_14160"
     CDS             complement(323..1099)
                     /locus_tag="PK242_14160"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95212.1"
                     /translation="MQVSRTLLAAAAVALFGLGPVAAQAAPPYVIVAGTGAVGADVGT
                     FPPGTSGTIAELGVDWQVTATAGATTGYHVYNGVQTWSFTRNGAPETVNLEFAISGID
                     AWLTTGGAVATEAIGLPVGTVCDISNPAYVFPAGALDGTPGGPVFSWTPDNGSGLAVL
                     QYLGNLGGVPVNRYLQYAPVPCTYTGSQLVLTGSGFPANEAGGTSFWHGLAYLRVMNQ
                     PVPPAAPTPVPTLDFAGLGLLSLLGAGAGALGLRRRKRSK"
     gene            complement(1206..>1850)
                     /locus_tag="PK242_14165"
     CDS             complement(1206..>1850)
                     /locus_tag="PK242_14165"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95213.1"
                     /translation="LEIDQTTGASTDVGAVSGLPAGSYNSGAFSNTGAYYVKPFSNNS
                     QLYRIDVTASPPTATLITLSQAFTTSDMAWVGSLMYSTADNGQLYSIDVATGTTTAIG
                     SPDNTGGVLGAQFSGTNGLFGSANNGSGLYRINLTTGQRDKISDSPASGANDGASCPT
                     VALFEPLPPEPPEPPEPPPPPTPVPTLDFPGLGLLSLLGAGAGALGLRRRKRTH"
BASE COUNT          358 a          576 c          646 g          270 t
ORIGIN      
        1 cggcggccac agcgtgatcg tgatcgagca cgacctggac ctgatcgccg aagccgactg
       61 gatcatcgac ctgggccccg aaggcggcga cggcggcggc cgcatcgtcg ccgtcggcac
      121 gcccgaggac gtggtgcgcg ccggcacgca caccggggtg gcgctggggg cggtgttggg
      181 gcgcaccccg atggcgccgg ggtgaggcac atgcgacagc gccccacacc cccgagatga
      241 gcccaacaaa aagccgcgtg gcaacccgta ggtcgccacg cggcgttcgc ggtgaacgcc
      301 cgatgaagac gggcgccgac gcttacttgc tgcgcttgcg gcggcgcagc cccagggcgc
      361 cagcgccagc acccagcagg ctcagcaggc ccaggccagc aaagtccagc gtgggtacgg
      421 gcgtgggggc agctggcggc actggttggt tcatgacccg aagataagcc aggccatgcc
      481 agaacgacgt gccgccggct tcgttggccg ggaacccaga gcccgtcagc accagttggc
      541 tgccggtgta ggtgcaggga acgggggcat attgaagata gcgattgact ggcacaccgc
      601 ccaaattgcc aagatattgc agcacggcaa ggcctgagcc attgtctgga gtccaggaaa
      661 aaacgggccc tcccggcgtt ccatccaacg cgccagcagg gaaaacatag gctgggttgc
      721 tgatatcgca cacggtgccc accggaagac caatggcttc tgtcgcgacc gcaccgcccg
      781 tggtaagcca cgcatcaatc ccgctgatgg cgaattcaag attcaccgtt tcgggcgcac
      841 cgttgcgcgt gaaagaccag gtctgcaccc cgttgtagac gtgatagccg gtggtggcgc
      901 cagcggtagc cgtcacttgc cagtccaccc ccagctccgc aatcgtgccc gatgtgcctg
      961 gcgggaacgt gcccacatcg gccccaacgg cacctgtgcc agccacgatg acgtatggcg
     1021 gggccgcctg cgcagcgacc ggccccaagc caaacaacgc aacggccgcg gccgcaagca
     1081 aagtacggga aacttgcatc tcttgatcct aagaaaagtg gagaagtgac agaacgaaag
     1141 gtccagaagc aggagccgga gcgctgcgag acggtgcctg aacgtcgtcg caacgctcca
     1201 actgctcagt gcgtgcgctt gcggcggcgc agccccaggg cgccggcacc agcacccagc
     1261 aggctcagca ggcccaggcc ggggaagtcc agcgtgggta cgggcgtggg gggcggtggc
     1321 ggctcgggcg gctcgggcgg ctcgggtggc aaaggctcga acagcgccac cgtggggcaa
     1381 ctggcaccgt cgttggcccc cgaagcgggc gaatcggaaa tcttgtcccg ctggcccgtg
     1441 gtcaggttga tgcggtacag gcccgagcca ttgttggccg agccgaacag accattggtg
     1501 cccgaaaact gcgcgcccag cacgccgcca gtgttgtcgg gcgagccaat ggctgtcgtg
     1561 gtgccagtcg cgacgtcgat ggagtaaagc tgtccgttgt cggcggtgga atacatcagg
     1621 ctaccgaccc aggccatgtc ggaggtggtg aaggcctgtg agagcgtaat cagcgtagcc
     1681 gtgggcggcg acgcagtcac gtcgattcgg tagagctggc tgttgttgct aaaaggcttg
     1741 acgtaatagg caccggtgtt gctgaaggcg ccgctgttgt acgagccggc gggaagaccc
     1801 gaaacggcgc cgacatcggt ggaggctccg gtggtctggt cgatttccag
//
LOCUS       DAOOOG010000339         1837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_80905_length_1837_cov_27.663861, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000339 DAOOOG010000000
VERSION     DAOOOG010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80905_length_1837_cov_27.663861"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..742)
                     /locus_tag="PK242_14170"
     CDS             complement(44..742)
                     /locus_tag="PK242_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOZ95214.1"
                     /translation="MRGPRGFTLIELLVALSLVAVIAVLSWRGIDAMARAQQSTQQHT
                     DEVLALQAGLAQWRADLDALMSWPAAPAAPGQPPASGPASQRSLAWDGRTLRLTRSDA
                     GDAAAGLRVVAWTRRPASGQWQRWQSPPLASQAAWAAAWDAAAHWGQSDTEPPAAAGA
                     SAVTLARALDWQLHYYRHNAWTHPLSSSAESGSATQLLPDGVRLFLTLAAGQALAGPL
                     VLDWVRPDFSGAQP"
     gene            complement(835..1212)
                     /gene="gspI"
                     /locus_tag="PK242_14175"
     CDS             complement(835..1212)
                     /gene="gspI"
                     /locus_tag="PK242_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HOZ95215.1"
                     /translation="MSRRRAAAPPDAGFTLVEVLVALAITAIALIAGFKASAALTDNA
                     GRQGTVLLAQICAENRLTELRLARQLPAVGDSELACAQAERQFLVTQAVRPTPNPNFR
                     RVEAAVSEAGVPVLRLSTIVGRH"
     gene            complement(1209..1643)
                     /locus_tag="PK242_14180"
     CDS             complement(1209..1643)
                     /locus_tag="PK242_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOZ95216.1"
                     /translation="MKSRRGFTLIELMVVIAIVAIGSALASLALPDSDRRTLAREGER
                     LAALLEAARAQSRAAGVPVRWRATASGFAWDGLPPGAAPLPTGWLDARTRALGNAALL
                     LGPDPIIDAQAVVLTRPDQHSPQALRIASDGLRPFGVAPLTP"
BASE COUNT          211 a          647 c          746 g          233 t
ORIGIN      
        1 tggcccccac gcttgcccgc tgcgcgtggc gcctggtggc gcgtcatggc tgcgctccgc
       61 tgaaatccgg ccgcacccag tcgagcacca gcgggccggc cagcgcctgg cctgcggcca
      121 gggtgaggaa caggcgcacg ccgtcgggca gcagctgggt ggcgctgccc gattcggcgc
      181 tgctggacag tgggtgcgtc caggcgttgt ggcggtagta gtgcaactgc cagtcgagcg
      241 cgcgcgcgag ggtgacggcg ctggcgccgg cggcggcggg cggttcggtg tcggactggc
      301 cccagtgcgc ggcggcgtcc caggcggcgg cccaggcggc ctgcgacgcc agcggcggcg
      361 attgccagcg ctgccactgg ccgctggcgg ggcggcgcgt ccaggccacc acgcgcaggc
      421 cggcggccgc gtcgccggcg tcgctgcggg tcaggcgcag ggtgcggccg tcccaggcca
      481 ggctgcgctg gctggccggg ccgctggcgg gcggctggcc gggggcggcg ggggcggcgg
      541 gccagctcat cagggcgtcg aggtcggcgc gccactgcgc cagtccggcc tgcagggcca
      601 gcacttcgtc ggtgtgctgc tgggtggact gctgcgcgcg cgccatggcg tcgatgccgc
      661 gccagctcag cacggcgatc acggcgacca ggctgagcgc caccagcagc tcgatcaggg
      721 tgaagccgcg cgggccgcgc aatgatgagc caaaccggca gccagcgctt gccaggcttg
      781 cgccgtcagc tatcgtaaca atagcgttcg agggtgggtt gcccacggcc ggcatcagtg
      841 gcgacccacg atggtgctca ggcgcagcac cggcacgccg gcttcgctga cggcggcttc
      901 gacgcggcgg aagttggggt tgggcgtggg ccgcacggcc tgcgtgacca ggaactggcg
      961 ctcggcctgg gcgcaggcca gttcgctgtc gccgacggcg ggcagctggc gcgccaggcg
     1021 cagctcggtc aggcggtttt cggcgcagat ctgggccagc agcaccgtgc cctggcggcc
     1081 ggcgttgtcg gtcagcgcgg cgctggcctt gaagccggcg atcagcgcga tggcggtgat
     1141 ggccagcgcc accagcactt ccaccagcgt gaagccggcg tcgggcggcg cggcggcgcg
     1201 gcggcggctc atggcgtcag cggcgcgacg ccgaagggcc gcaggccgtc gctggcgatg
     1261 cgcagcgcct gcgggctgtg ctggtcgggc cgcgtcagca ccaccgcctg cgcgtcgatg
     1321 atcgggtcgg ggcccagcag cagggcggcg ttgcccaggg cgcgggtgcg cgcgtccagc
     1381 cagccggtgg gcagcggcgc ggcgccgggc ggcaggccgt cccaggcaaa gccgctggcg
     1441 gtggcgcgcc agcgcaccgg cacgccggcg gcgcgcgact gcgcgcgggc cgcttcgagc
     1501 agcgccgcca gccgttcgcc ttcgcgcgcc agcgtgcggc ggtcgctgtc gggcagggcc
     1561 aggctggcca gcgcactgcc gatggcgacg atggcgatca ccaccatcag ctcgatgagg
     1621 gtgaagccgc gccgggattt catgccaaag tagactgtgg cgcccgtcca tgcagcgccg
     1681 gcagctcctg tttcaggagc gaccgggggc atgctcagcc ccgccggcgc cgcgccgtgg
     1741 cggcggcgcg caggcaccgg ccagccgctc gggcgagcgc cttcactgcc agctgccgat
     1801 gtcggcatcc ttgccttcgc cgccgctctt gccgtcg
//
LOCUS       DAOOOG010000340         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_82399_length_1816_cov_30.533220, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000340 DAOOOG010000000
VERSION     DAOOOG010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82399_length_1816_cov_30.533220"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PK242_14185"
     CDS             complement(<1..935)
                     /locus_tag="PK242_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOZ95217.1"
                     /translation="MRFSEPPPTPAALGRWRGHALTALLAGGVLGVAAQLQQARLWPA
                     WAYALTGLLALLLAVVAWRPRRAGARALGLWLLAGALAAGASTGWRAGLRQAERLAPA
                     LEGRDLRLTGRIAGLPQRGEAGWRFRFDVERASLDGAPVRVPPRVQLGWYGWVATVDE
                     TDAERPVAAALRSGDRWQLTARLKAPHGNLNPHGFDLELWLWEQGVGASGYVRSGPRE
                     APARRLEEGGASLPRWRQAVRDRILDGADAGDATARRLAGIVAALVTGDQNAIERADW
                     DVFRATGVAHLMSISGLHITMFAWLAAALVGALWRR"
     gene            1164..1622
                     /locus_tag="PK242_14190"
     CDS             1164..1622
                     /locus_tag="PK242_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOZ95218.1"
                     /translation="MTHPAEQLKALGISLPPVSTPAAAYVPFVRSGPLVFLSGHIARR
                     DGQPWVGKLGASMTTAEGAQAARAVAIDLMGTLQAAVGDLGKVRRIVKVMSLVNSTPD
                     FTEQHLVTNGCSELLGQVFGDAGKHARSAFGVAQIPLGACVEIELIAEVD"
BASE COUNT          243 a          739 c          640 g          194 t
ORIGIN      
        1 cgccgccaca acgcacccac cagcgccgcc gccagccagg cgaacatggt gatgtgcagg
       61 ccggagatgc tcatcaggtg cgccacgccg gtggcgcgaa acacgtccca gtcggcgcgc
      121 tcgatggcgt tctgatcgcc cgtcaccagc gccgccacga tgccggccag ccggcgcgcc
      181 gtggcgtcgc cggcgtcggc gccgtccagg atgcggtcgc gcaccgcctg ccgccagcgc
      241 ggcagcgagg cgccgccttc ctccagccgg cgcgccggcg cctcgcgcgg accgctgcgc
      301 acgtagccgc tggcgcccac gccctgctcc cacagccaca gctccaggtc gaagccgtgc
      361 gggttcaggt tgccgtgcgg cgccttcagc cgcgccgtca gctgccagcg gtcgccactg
      421 cgcagcgccg cggccacggg gcgctcggcg tcggtttcgt cgaccgtcgc cacccagccg
      481 taccagccca gctgcacgcg cggcggcacg cgcaccggcg cgccgtccag gctggcccgc
      541 tcgacgtcga agcgaaagcg ccagccggct tcgccgcgct gcggcaggcc ggcgatgcgg
      601 ccggtcagcc gcaggtcgcg cccttccagc gccggcgcca gccgctcggc ctggcgcagc
      661 ccggcgcgcc agccggtgct ggcgccggcg gccagcgccc cggccagcag ccacagcccg
      721 agcgcgcgcg ccccggcccg gcgcgggcgc caggccacca ccgccagcag cagcgccagc
      781 aggcccgtca gcgcataggc ccaggccggc cacaggcgcg cctgctgcag ctgcgccgcc
      841 acgcccagca cgccgcccgc cagcagcgcc gtcagcgcat ggccgcgcca gcgcccgagc
      901 gcggcagggg ttggcggggg ttcggaaaac cgcatcggac gctatggctt cgatagcttc
      961 tggcgcagac cagataagcg ccagggccgc ttgggagcct aagcccgcga gcgcgccggg
     1021 ccagcgcctg ggtgccagta tctccgcaaa cgccgcgccg gcgtggcgcc gcgcccacgc
     1081 cagccggtgc gacaatggcc gccccgcggt ccgagccgtt gccaccgctc ggtgatccgc
     1141 cccttcttcc ggagaccccg accatgaccc atcccgccga gcagctcaaa gccctgggca
     1201 tcagcctgcc gcccgtcagc acccccgccg ccgcctacgt gcccttcgtg cgcagcggcc
     1261 cactggtgtt cctgtccggc cacatcgcgc gccgggacgg ccagccctgg gtcggcaagc
     1321 tgggcgccag catgaccacc gccgaagggg cgcaggccgc gcgcgcagtc gccatcgacc
     1381 tgatgggcac gctgcaggcg gcggtgggcg atctgggcaa ggtgcgccgc atcgtcaagg
     1441 tgatgagcct ggtcaacagc acgcccgact tcaccgagca gcacctggtc accaacggct
     1501 gctccgagct gctgggccag gtgttcggtg acgccggcaa gcacgcgcgc agcgccttcg
     1561 gcgtggcgca gatcccgctc ggcgcctgcg tcgagatcga gctgatcgcc gaagtcgact
     1621 gatcggttcc ggcagcggcg gcgccgccgt gcccgaagcc ggcgtgggcc gggccgggcg
     1681 ggtggttcgg tctcaggccg acggtggctg cgccgccccg cccagggccg gcacgctgcg
     1741 cgcgcccagc gccagcacca gcgccaccag ccagaacaga ccgccggtgc cgatgacggc
     1801 gccgatggag ccgaac
//
LOCUS       DAOOOG010000341         1815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_82460_length_1815_cov_43.446023, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000341 DAOOOG010000000
VERSION     DAOOOG010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82460_length_1815_cov_43.446023"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PK242_14195"
     CDS             <1..185
                     /locus_tag="PK242_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HOZ95219.1"
                     /translation="EHRILRRAEVEAKTGFKRAHIYSLMKEGKFPKALRLGVRAVGWD
                     SVEIEQWIADRLKERA"
     gene            226..1092
                     /locus_tag="PK242_14200"
     CDS             226..1092
                     /locus_tag="PK242_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009401136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOZ95220.1"
                     /translation="MQVVSIISTKGGVGKTTTAANLGGFIADAGLRVLLLDLDVQPTL
                     SSYFTLDVRAPGGIYEMLAFNERRIEQLVSRTAIAGLDLVLSNDDRGELNTLLLHAPD
                     GRLRLRHLLPVFRTHYDLLLIDTQGARSVLLEMAVLASDLALSPVTPEILAARELRRG
                     TLQLIEDIAPYRHLGIEPPPLRLLINRVHPVSSNARLVQQALRQVFQEQAGVQVLDTD
                     VPAIEAYPRAATRGLPVHRVEYRQPAGRTAPAALETMRTLAGELFPAWRERFALVTGR
                     ADAGGAGHGERA"
     gene            1076..1312
                     /locus_tag="PK242_14205"
     CDS             1076..1312
                     /locus_tag="PK242_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOZ95221.1"
                     /translation="MASAHELARGRERLRALIEFALGEGWRVVRTSGGHLKFTKPGCA
                     SIYTSSTASDHRADRNARAQLRRADRQAQENGRG"
     gene            1305..>1815
                     /locus_tag="PK242_14210"
     CDS             1305..>1815
                     /locus_tag="PK242_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOZ95222.1"
                     /translation="MAELTPQDMAAKLLATGFERSGPSAATLSDPIADTPMVVTLDQL
                     RPYDHDPRVTRNPAYAEIKASIRERGLDAPPAITRRPGEAHYIIRNGGNTRLAILREL
                     WSETKEERFFRIACLFRPWPARGEIVALTGHLAENELRGGLTFIERALGIEKAREFYE
                     QESGQALSQS"
BASE COUNT          290 a          622 c          646 g          257 t
ORIGIN      
        1 ccgagcaccg catcctgcgc cgcgccgagg tcgaagccaa gaccggcttc aagcgcgcgc
       61 acatctacag cctgatgaag gaaggcaagt tccccaaggc gctgcgcctg ggcgtgcgcg
      121 cggtgggctg ggactcggtg gagatcgaac agtggatcgc cgatcgcctc aaagaacgcg
      181 cctgacgctt cttctcggtt catgccattc gacgaggaga agcccatgca ggtggtgtcc
      241 atcatttcga ccaagggcgg cgtgggcaag accacgacgg cggccaacct gggcggcttc
      301 atcgccgatg ccgggctgcg cgtgctgctg ctggacctgg acgtgcagcc cacgctgtcg
      361 agctacttca cgctggacgt gcgcgcgccc ggcggcatct acgagatgct ggccttcaac
      421 gagcggcgca tcgagcaact ggtgtcgcgc accgcgatcg cgggcctgga cctggtgctc
      481 tccaacgacg accgcggcga actgaacacg ctgctgctgc acgctccgga tgggcgcctg
      541 cgactgcgcc atctgcttcc cgtcttccgc acgcactacg acttgctgct gatcgacacc
      601 cagggcgcgc gcagcgtgct gctggagatg gcggtgctgg cgtccgacct ggcgctgtcg
      661 ccggtgacgc cggaaatcct cgcggcgcgc gagctgcggc gcggcacgct gcaactgatc
      721 gaggacatcg cgccgtatcg gcacctgggc atcgagccgc cgccgctgcg cctgctcatc
      781 aaccgtgtgc atccggtgtc gtcgaacgcg cggctggtcc agcaggcgct gcgacaggtg
      841 ttccaggaac aggccggcgt gcaggtgctg gacaccgacg tgccggccat cgaagcctat
      901 ccgcgcgctg cgacacgagg attgccggtg caccgggtgg agtaccggca gccggctggg
      961 cgcacggcgc ccgcggcgct ggagaccatg cgcacgctgg ccggcgagct gttccccgcg
     1021 tggcgggagc gcttcgcgct ggtcaccggc cgggccgatg cgggaggggc cggccatggc
     1081 gagcgcgcat gaactggcgc gcggccgcga acggctgcgc gcgctgatcg agttcgcgct
     1141 gggcgaaggc tggcgcgtgg tgcgcacgtc cggcgggcac ctgaaattca cgaaaccagg
     1201 ctgcgcgtcg atctacacca gctcgactgc gagcgaccac cgtgccgacc gcaatgcccg
     1261 cgcgcaactt cgccgcgccg accggcaggc gcaggagaac ggccgtggct gagctgacgc
     1321 cgcaggacat ggctgccaag ctgctggcca ccggcttcga gcgcagcggc ccttcggccg
     1381 cgaccttgag cgaccccatc gccgacacgc cgatggtggt gacgctggac cagttgcggc
     1441 cctacgacca cgacccgcgc gtgacgcgca acccggccta tgcggagatc aaggcgtcca
     1501 tccgcgaacg cgggctggac gcgccccccg cgatcacgcg caggccgggc gaggcgcact
     1561 acatcattcg caacggcggc aacacgcggc tggcgatcct gcgcgagttg tggagcgaga
     1621 ccaaggagga acgcttcttc cgcattgcgt gcctgttccg cccgtggccg gcgcgcggcg
     1681 aaatcgtggc gctgaccggg catctggccg agaacgagct gcgcggcggg ctgaccttca
     1741 tcgagcgggc cttgggcatc gagaaggcgc gcgagttcta cgagcaggaa agcggccagg
     1801 cgctgtcgca gagcg
//
LOCUS       DAOOOG010000342         1813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_82591_length_1813_cov_40.141638, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000342 DAOOOG010000000
VERSION     DAOOOG010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82591_length_1813_cov_40.141638"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PK242_14215"
     CDS             <1..546
                     /locus_tag="PK242_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOZ95223.1"
                     /translation="AIGVAALSTAAMVLYPLVAQALQLDARLAGLFLGGAIHDVAQVV
                     GAGAILSPEVAQSATLVKMFRVAMLVPVVLCVALTFRAPAHAAGQPRPRGPALLPGFL
                     LAFIALVVANSLGAIPAPLQSAAAGVSAWALVVAIAALGVKTSFEKIVRLGWRPIALL
                     VSETLFIALFMLLAVGWQARS"
     gene            complement(551..>1813)
                     /locus_tag="PK242_14220"
     CDS             complement(551..>1813)
                     /locus_tag="PK242_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOZ95224.1"
                     /translation="AACQNWRLRDEELAHCLALVTPRLILASPRHAEAARRCGGAVPL
                     LCLGAEYEQALQAATAEPVEDRCDPEDGVVILYTSGTTGMPKGALISQRAEIARAAIQ
                     AMDLPHSADEHFVAWAPLFHMVSTDTVFMTLMQGGKVIVTDGFDADELAAIVARERLG
                     RLTLMPGMITPFVDAMRRLGAPPAGVKWVGVMADLVPREQIAEVTRLLGAPYINSFGA
                     TETGYPPASLATIPVGVVPTSLDKQQSSLCRIRLVDADDREVPDGEPGELTIRTPALF
                     SGYWNNPDANREDFRGGWFHMGDVFRRNPDGTLSFVDRRKYLIKSGGENIYPAEIERV
                     LLSEPGVADAVVVRRPDPTWGEVPVAFVVRRDPTLDAAALQALCRQQIAGYKVPKEVH
                     FVDDAALPRSTTGKIMRHELEQRLPPVA"
BASE COUNT          229 a          630 c          669 g          285 t
ORIGIN      
        1 gccatcggcg tggccgcgct gtccaccgcc gccatggtgc tgtacccgct ggtggcgcag
       61 gcgctgcagc tggacgcacg gctggcgggg ctgtttctgg gtggcgccat ccatgacgtg
      121 gcgcaggtgg tgggcgccgg cgccatcctg tcgcccgagg tggcgcagtc ggccaccctg
      181 gtcaagatgt tccgcgtcgc catgctggtg ccggtggtgc tgtgcgtggc gctgaccttt
      241 cgcgcgccag cgcacgcggc cggccagccg cgcccgcgcg ggccggcgct gctgccgggc
      301 tttctgctgg ccttcatcgc cctggtggtg gccaacagcc tgggcgccat cccggcgccg
      361 ctgcagtcgg cggcggccgg agtgtcggcc tgggcgctgg tggtggcgat tgccgcgctg
      421 ggcgtgaaga cctcgttcga aaagatcgtg cgcctgggct ggcgaccgat cgcgctgctg
      481 gtgagcgaga cgctgttcat cgccctgttc atgctgctgg ccgtggggtg gcaggcccgc
      541 agctgaaaac tcacgccacc ggcggcaggc gctgctccag ctcgtggcgc atgattttgc
      601 cggtggtgct gcgcggcagc gcggcgtcgt cgacgaagtg cacttccttg ggcaccttgt
      661 agccggcgat ctgctgacgg cacagcgcct gcagcgcggc ggcgtccagc gtcgggtcgc
      721 gccgcaccac gaaggccacc ggcacctcgc cccaggtcgg gtcgggtcgg cgcaccacca
      781 cggcgtcggc cacgcccggc tccgacagca gcacgcgctc gatttcggcc gggtagatgt
      841 tttcgccgcc cgacttgatg aggtacttgc gccggtcgac gaagctgagc gtgccgtccg
      901 gattgcgccg aaacacgtcg cccatgtgga accaaccacc gcgaaagtcc tcgcggttgg
      961 cgtccgggtt gttccagtag cccgagaaca gcgccggcgt gcggatggtc agctcgcccg
     1021 gctcgccatc gggcacctcg cggtcgtcgg catcgaccag gcggatgcgg cacagcgaac
     1081 tctgctgctt gtccagactg gtcggcacca cgccgaccgg gatggtggcc agcgaagccg
     1141 gcgggtagcc ggtttcggtg gcgccgaagg agttgatgta gggcgcgccc agcagccgcg
     1201 tgacctcggc gatctgctcg cgcggcacca ggtcggccat cacgcccacc cacttgacgc
     1261 ccgccggcgg cgcgcccagg cgccgcatgg cgtcgacgaa gggcgtgatc atgccgggca
     1321 tcagggtcag ccgccccagg cgctcgcgcg ccacgatggc ggccagttcg tcggcgtcga
     1381 agccgtcggt gacgatcacc ttgccgccct gcatcagggt catgaacacg gtgtcggtgg
     1441 acaccatgtg aaacagcggc gcccaggcca cgaagtgctc gtcggcgctg tgcggcaggt
     1501 ccatggcctg gatggcggca cgggcgattt cggcgcgctg gctgatcagc gcccccttgg
     1561 gcatgccggt ggtgccgctg gtgtagagga tcaccacccc atcctcgggg tcgcagcggt
     1621 cttccaccgg ctcggcggtg gcggcctgca gcgcctgctc gtactcggcg cccaagcaca
     1681 gcagcggcac cgcgccgccg cagcggcgcg ccgcctcggc gtggcgcggc gaggccagga
     1741 tcagtcgcgg cgtgaccagc gccaggcagt gggccagctc ttcgtcgcgc aggcgccagt
     1801 tctggcaggc ggc
//
LOCUS       DAOOOG010000343         1806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_83128_length_1806_cov_32.902342, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000343 DAOOOG010000000
VERSION     DAOOOG010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83128_length_1806_cov_32.902342"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="PK242_14225"
     CDS             complement(<1..1516)
                     /locus_tag="PK242_14225"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOZ95225.1"
                     /translation="MDCASEEAQIRRALEGQAAVRGLRFDLGARTLAVDAPDSAWPAV
                     EQAIAAAGLKTERLSQPTSAADTRRRQRRQATQLAAALLLALGAELLHFVAPDTRPWQ
                     FASMALAALAIALSGFGVYRKGLAALARGQLGISALMTVAVTGAFLIGQWTEAAMVMA
                     LYSIAELIEARAVDRARHAIGGLLALTPPLAEVRQPDGGWLTQRADSVASGALVRVRP
                     GERLALDGRVTAGHSAIDQAPITGESVPVEKAPGDEVFAGTINQHGALEYEVTAPASD
                     TVLARIIHAVEQAQGTRAPTQRLVDRFAAVYTPTVFAIALAVALLGPWLAGWPWLTAL
                     YKALVLLVIACPCALVIATPVTVVSGLAAAARRGILIKGGAYLEEARRLRHIALDKTG
                     TLTEGRPRLVARELVDDRQEEARVWQLAASLAARSDHPVSRAIAQGLHDAGTLAVDDF
                     GAAAGRGTNGRIHDQALVLGNHRWIEERGQCSPALEARMQAHEALGRSVSLLADD"
BASE COUNT          230 a          658 c          671 g          247 t
ORIGIN      
        1 ggtcgtcggc cagcaggctg acgctgcggc ccagcgcctc gtgcgcctgc atgcgcgctt
       61 ccagcgcggg cgaacactgg ccgcgctcct cgatccagcg gtggttgccc agcaccagcg
      121 cctggtcgtg gatgcggcca ttggtgccgc gcccggcggc ggcgccgaag tcgtccaccg
      181 ccagcgtccc ggcgtcgtgc aggccctggg cgatggcgcg cgacaccggg tggtcggagc
      241 gcgccgccag gctggcggcc agctgccaga cgcgggcttc ttcctgccgg tcatcgacca
      301 gctcgcgcgc caccaggcgc ggccgccctt cggtcagggt gccggtcttg tccagcgcga
      361 tgtggcgcag ccgacgcgct tcttccaggt acgcgccgcc cttgatcaga atgccgcgcc
      421 gtgccgccgc tgccagaccg ctgaccaccg tcaccggcgt ggcgatgacc agggcgcagg
      481 ggcaggcgat gaccagcagc accagcgcct tgtaaagcgc cgtcagccac ggccagccgg
      541 ccagccacgg cccgagcagc gccaccgcca gcgcgatggc gaacaccgtc ggggtataga
      601 cggcggcaaa gcggtccacc aggcgctgcg tcggcgcgcg cgtgccctgg gcctgctcga
      661 ccgcgtggat gatgcgcgcc agcaccgtgt cgctggccgg cgcggtgact tcgtattcca
      721 gcgcgccgtg ctggttgatg gtgccggcaa acacctcgtc gccgggcgcc ttctcgaccg
      781 gcacgctttc gccggtgatg ggggcctgat cgatggcgct gtggccggcg gtcacgcgcc
      841 cgtccagcgc cagccgctcg cccgggcgca cgcgcaccag cgcaccgctc gccacgctgt
      901 cggcgcgctg cgtcagccag ccgccgtccg gctggcgcac ctcagccagc ggcggcgtca
      961 gcgccagcag gccgccgatg gcgtggcgcg cgcgatcgac ggcgcgcgcc tcgatcagct
     1021 cggcgatgga atacagcgcc atcaccatgg cggcttcggt ccactggccg atcaggaagg
     1081 cgccggtcac ggccaccgtc atcagggccg aaatgcccag ctggccgcgc gccagcgccg
     1141 ccaacccctt gcggtagaca ccaaagcccg acagcgcaat ggccagcgcc gccagcgcca
     1201 tgctggcgaa ctgccagggc cgggtgtcgg gggcgacgaa gtgcagcagc tcggcgccca
     1261 gggccagcag cagggcggcg gccagctggg tggcctggcg gcgctggcgg cggcgcgtgt
     1321 cggcggcgct ggtcggctgg ctcaggcgct cggttttcag gccggcggcg gcgatggcct
     1381 gctcgaccgc cggccaggcg ctgtcgggcg cgtccactgc cagcgtgcgc gcgcccaggt
     1441 cgaagcgcag gccgcgcacc gcggcctggc cctccagcgc gcggcggatc tgggcttcct
     1501 cgctggcgca gtccatgtcg ggaatgcgca gcagcagggc gccgcccgcc gccagcgccg
     1561 agaagtcgat cggccgcgcc gccacttcac aggcggcgcc gccgcaatcg tggtcggcgt
     1621 ggggatggtc gtgcccatgc tcatgcgcgt ggtcgtgcgc gtggcggtgc gggtggacgt
     1681 gggggtgggc ggtgctgctg ctcatggggc gattcggtgg cggtctggcg aaccttcggg
     1741 tatattgaaa gcgctgtagt cactacagag tcaagcattg cgttgtctta accccacccc
     1801 aggtgc
//
LOCUS       DAOOOG010000344         1794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_83981_length_1794_cov_31.961472, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000344 DAOOOG010000000
VERSION     DAOOOG010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83981_length_1794_cov_31.961472"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PK242_14230"
     CDS             complement(<1..398)
                     /locus_tag="PK242_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95226.1"
                     /translation="MDSEIRNADAVAAEPLNPACPELHRWGARQLYGRWSVELTELGQ
                     RGELVLRQHPEFLESLRGEFVIDGQISIASGDVDQGEFNLDESRDGKSLFAFWNGRLV
                     PAACGREIRGSWQSLPQPGVPARESSFVLRR"
     gene            complement(472..>1794)
                     /locus_tag="PK242_14235"
     CDS             complement(472..>1794)
                     /locus_tag="PK242_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOZ95227.1"
                     /translation="IYGWRGATLDNLRRLPQDYPRLKVIKLEQNYRSTSAILRAANNV
                     IGPNPKLFEKRLFSELGEGEPVRVLACDHEQHEAERAVARIQSLRQTSAHKDWRDFAI
                     LYRANHQAKVFEQALRKAQIPYKVSGGQSFFDRAEIKDLCAWLRLWINEDDDPAFLRA
                     VTTPKRGIGHQSLAALGEFAGKARLSLFGALFAHSLPAALPARTIASLHEFGRLVNDL
                     RHRAGHTHGHEAARTFLADWLKDIGYEQHLHDSADSAPQAAARWSNVLEFCDWMAGRC
                     GGQIEDAAGVTLHQESRSLLEVAQTISLISTLNEREQDPNVVTLSTLHAAKGLEWPHV
                     VLAGVNEGLLPFRPDEDAGAAAQAARIEEERRLMYVGITRARNTLAVSWTRRRKKGRE
                     VVAAQPSRFIAEMALDAATVREDPRAKLKALRAEFAQRAASAPPQAAP"
BASE COUNT          251 a          587 c          662 g          294 t
ORIGIN      
        1 cgccgcagca cgaagctgct ctcgcgggcg ggcacgccgg gctggggcag ggattgccag
       61 ctgccgcgga tttcgcggcc gcaggcggcg ggcaccaggc ggccgttcca gaaggcgaac
      121 aggcttttac cgtcgcgcga ctcgtccagg ttgaactcgc cctggtccac atcgcccgag
      181 gcgatcgaga tctggccgtc gatgacgaac tcgccgcgca ggctttccag aaattcgggg
      241 tgctggcgca acaccagctc gccgcgctgg cccagctcgg tcagttccac gctccagcgg
      301 ccataaagct ggcgcgcacc ccagcggtgg agctcggggc aggccgggtt gagcggctcg
      361 gcagcgaccg catctgcgtt cctgatttcg gagtccatgg ggcctgtagc gcaggcggag
      421 agtgcgccgg cagctaccaa aagaatagcg tatcggatca ttggagcgac ttcatggggc
      481 cgcctgcggc ggtgcactgg cggcgcgctg ggcaaactct gcgcgcagcg ccttgagctt
      541 ggcacgcggg tcctcgcgca cggtggctgc gtccagcgcc atttcggcga tgaagcggct
      601 gggctgggcg gccaccacct cgcggccctt cttgcgccgg cgcgtccagc tcacggccag
      661 ggtgttgcgg gcgcgggtga tgccgacgta catcagacgg cgctcttcct cgatgcgcgc
      721 ggcctgggcg gcggcgccgg cgtcctcgtc ggggcgaaac ggcagcaggc cttcgttgac
      781 gccagccagc accacgtgcg gccactccag gcccttggcg gcgtgcaggg tggacagggt
      841 gacgacgttg gggtcctgct cgcgttcgtt cagcgtggag atcagcgaga tggtctgcgc
      901 gacttcgagc aggctcctgg attcctggtg cagcgtgacg cccgcggcgt cctcgatctg
      961 gccgccgcag cgcccggcca tccagtcgca gaactccagc acgttggacc agcgcgcggc
     1021 ggcctgcggg gcgctgtcgg cgctgtcatg caggtgctgc tcgtagccga tgtccttcag
     1081 ccagtcggcc agaaaagtgc gcgccgcctc gtggccgtgg gtgtggccgg cgcggtggcg
     1141 caggtcgttc acgaggcgcc cgaactcgtg caggctggcg atggtgcgcg ccggcagcgc
     1201 ggccggcagc gagtgcgcaa acagcgcgcc aaacagcgac agtcgggcct tgccggcaaa
     1261 ttcgcccagc gcggccaggc tctggtggcc gatgccgcgc ttgggggtgg tgacggcgcg
     1321 cagaaaggcc gggtcgtcgt cctcgttgat ccacaggcgc agccaggcgc acaggtcctt
     1381 gatctcggcg cggtcgaaaa agctttgccc gcccgacacc ttgtagggga tctgcgcctt
     1441 gcgcagcgct tgttcgaaca ccttggcctg gtggttggcg cggtacagga tggcgaagtc
     1501 gcgccagtcc ttgtgcgcgg aggtctggcg cagcgactgg atgcgcgcca cggcgcgctc
     1561 ggcctcgtgc tgctcgtggt cgcaggccag cacgcgcacc ggctcgccct cgcccagctc
     1621 ggaaaacagg cgcttttcga acagcttggg gttggggccg atcacgttgt tggcggcgcg
     1681 caggatggcg gaagtcgacc ggtagttctg ctccagcttg atgaccttga gccgcgggta
     1741 gtcctgcggc agccggcgca ggttgtccag cgtggcgccg cgccagccgt agat
//
LOCUS       DAOOOG010000345         1788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_84419_length_1788_cov_24.510098, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000345 DAOOOG010000000
VERSION     DAOOOG010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84419_length_1788_cov_24.510098"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="PK242_14240"
     CDS             complement(<1..1571)
                     /locus_tag="PK242_14240"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HOZ95228.1"
                     /translation="MSDAAPTPATAPACIPQIRLYQNWLREQRGLSFASYRELWRWST
                     TELDAFWQSIWDYADLRSPTPHTAVLGRNVMPGAQWFPGAQVNYAQQVFRHVDAAHAA
                     GQPAVISDDEKGRVRELSWPELRRQVASLALHLQAQGVQPGDRVAAYLPNLPETMVAF
                     LATVSLGAIWSVCAPDMGTHAVLDRFRQIEPKVLIAVDGITWAGRDIDRSAVVRELRA
                     GLPSVQHLLLLRRSDTIETIADGADWTSATARNDAQTAAFEPRWLPMDHPLWIVYSSG
                     TTGLPKPIVHSHGGILLVAIATNLHMDVGSSYGANNFGERFHWYSSTGWVMWNCQVAG
                     LLSGATCVIYDGSPGGPRDAPDWGVLWRFAARHGVTFLGSGAAFFAGCAKAGVDLAQC
                     GDLSRVRALGSTGSPLSAEVQQWGTEQFRAIAKTEAQRDIWWFNISGGTDFAGGFIAG
                     HRELPQRPGVMQCRMLGSAVEAWNDAGEPVLGQVGELVCTQPIPSMPLCFWNDPGNAR
                     YLASYFEMYPPGHGR"
BASE COUNT          262 a          586 c          673 g          267 t
ORIGIN      
        1 ttgcggccgt ggccgggcgg gtacatctcg aagtagctgg ccagatagcg ggcgttgccg
       61 gggtcgttcc agaagcacag gggcatggac gggatgggct gggtgcagac caattcgccc
      121 acctggccca gcacgggttc gccggcgtcg ttccaggctt cgacggccga gccgagcatg
      181 cggcattgca tgacgccggg gcgctgcggc agttcgcggt ggccggcgat gaagccgccc
      241 gcaaaatccg tcccgccgga gatgttgaac caccagatat cgcgctgcgc ctctgttttg
      301 gcaatggcgc ggaactgctc ggtgccccat tgctgcactt cggccgacag cggcgagccg
      361 gtgctgccca gggcgcgcac gcgcgacagg tcgccgcact gggccagatc gacgccggcc
      421 ttggcgcagc cggcaaaaaa cgccgcgccc gagcccagaa aggtgacgcc gtggcgcgcc
      481 gcaaagcgcc acagcacacc ccagtcgggc gcgtcgcgcg ggccgcccgg gctgccgtcg
      541 tagatcacgc aggtggcgcc cgacagcagg ccggccacct ggcagttcca catcacccag
      601 ccggtggagc tgtaccagtg aaagcgctcg ccgaagttgt tggcgccgta gctgctgccg
      661 acgtccatgt gcaggttggt ggcgatggcc accagcagga tgccgccgtg cgagtgcacg
      721 atgggcttgg gcaggccggt ggtgccgctg gagtagacga tccacagcgg gtggtccatc
      781 ggcagccagc gcggctcgaa ggccgcggtt tgtgcgtcgt ttcgggctgt ggcgcttgtc
      841 cagtcagcgc cgtcagctat tgtttcgata gtgtcggagc ggcgcagcag cagcaggtgc
      901 tgcacgctgg gcaggccggc gcgcagctcg cgcacgacgg cgctgcggtc gatgtcgcgc
      961 ccggcccagg tgatgccgtc gacggcgatc agcaccttgg gctcgatctg cctgaagcgg
     1021 tcgagcacgg catgggtgcc catgtcgggc gcgcacacgc tccagatggc gccgaggctg
     1081 acggtggcca gaaaggcgac catggtttcg ggcaggttgg gcaggtaggc ggccacgcgg
     1141 tcgcccggct gcacgccctg ggcctgcagg tgcagcgcca gcgacgccac ctggcggcgc
     1201 agctcgggcc agctcagctc gcgcacgcgg cccttttcgt cgtcgctgat gacggccggc
     1261 tggccggcgg cgtgcgcggc atcgacgtgg cgaaacacct gctgcgcgta gttgacctgg
     1321 gcgcccggaa accactgggc gccgggcatc acgttgcgcc ccagcaccgc cgtgtgcggc
     1381 gtgggcgagc gcaggtcggc gtagtcccag atgctttgcc agaaggcgtc gagctcggtg
     1441 gtggaccagc gccacagctc gcggtagctg gcaaacgaca ggccgcgctg ctcgcgcagc
     1501 cagttctggt acaggcggat ctgcgggatg caggcggggg cggtggcggg ggtgggggcg
     1561 gcgtcggaca tgggccgatt attggcaagc cgcgccccgc gcgcagcggc tgaaagcggc
     1621 tttcggccta gggtttgtac ggttgttcaa ggggattgga cgatcgtcca atcgccccat
     1681 gcgcaccgtg ctgacccgtc ccgccgcgct ggcgccggcc gccgacgagg cggcgcgccc
     1741 cgaccgcaag gcggccatcc tgctggcggc cgagaagctg tttgccca
//
LOCUS       DAOOOG010000346         1771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_85648_length_1771_cov_31.674825, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000346 DAOOOG010000000
VERSION     DAOOOG010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85648_length_1771_cov_31.674825"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /gene="gap"
                     /locus_tag="PK242_14245"
     CDS             complement(<1..956)
                     /gene="gap"
                     /locus_tag="PK242_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOZ95229.1"
                     /translation="MTIKIGINGFGRIGRMVFRAAVANYKDIEVVGINDLLEPDYLAY
                     MLKYDSVHGVFAGDIAVEGNTLVVNGKKIRLTAERDPANLKWGEVGADVVIEATGLFL
                     TKEAAQKHLDAGARKVMMSAPSKDDTPMFVFGVNHDTYAGQAIISNASCTTNCLAPVA
                     KVLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VLPALKGKLTGMSFRVPTSDVSVVDLTVELDKPASYAEICAEMKAQSEGKLKGILGYT
                     EDKVVATDFRHDARSSIFDADAGIALDATFVKLVSWYDNEWG"
     gene            complement(1146..>1771)
                     /locus_tag="PK242_14250"
     CDS             complement(1146..>1771)
                     /locus_tag="PK242_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOZ95230.1"
                     /translation="HDSIGLGEDGPTHQAVEHAASLRLIPGLDVWRPADTAETVVAWA
                     VALQQRTLPTALLLSRQNLPYCPRRDLGDISRGAYVLSEPSDLGLKRKAQAVLIATGS
                     EVQLALKAQELLAREHRIAVRVVSMPSTTTFDRQELKYQKAVLPAGLPRIAVEMGATD
                     GWWKYGCAAVVGLDRFGESAPAPVLFEHFGFTPENVADTVRAALRRR"
BASE COUNT          264 a          578 c          607 g          322 t
ORIGIN      
        1 tagccccact cgttgtcgta ccagctcacc agcttgacga aggtggcatc cagcgcgatg
       61 ccggcgtcgg cgtcgaagat cgaggagcgc gcgtcgtggc ggaagtcggt ggccaccacc
      121 ttgtcttcgg tgtagcccag gatgcccttg agcttgcctt cgctctgggc cttcatctcg
      181 gcgcagatct cggcgtagct ggcgggcttg tccagctcca ccgtcaggtc gaccaccgag
      241 acgtcggagg tcggcacgcg gaagctcatg ccggtcagct tgcccttgag ggccggcagc
      301 accacgccga ccgccttggc ggcgccggtg gagctgggga tgatgttttc caggatgccg
      361 cggccgccgc gccagtcctt gttgctgggg ccgtccacgg ttttctgggt ggcggtggcg
      421 gcgtgcacgg tggtcatcag gccgcgcttg atgccccact tgtcgttcaa caccttggcc
      481 accggggcca ggcagttggt ggtgcagctg gcgttggaga tgatggcctg gccggcgtag
      541 gtgtcgtggt tgacgccgaa cacgaacatc ggcgtgtcgt ctttgctggg cgcgctcatc
      601 atcaccttgc gggcgccggc gtccaggtgc ttctgcgccg cttccttggt caggaacagg
      661 ccggtggctt cgatcaccac gtcggcgccg acctcgcccc acttcaggtt ggcggggtcg
      721 cgctcggccg tcaggcggat cttcttgccg ttcaccacca gggtgttgcc ctcgaccgcg
      781 atgtcgcccg caaacacgcc gtgcacgctg tcgtacttga gcatgtaggc caggtagtcg
      841 ggctccagca ggtcgttgat gccgacgacc tcgatgtcct tgtagttggc gacggccgcg
      901 cgaaacacca tgcgcccgat gcgcccgaag ccgttgatac cgatcttgat ggtcatgaca
      961 gtttccttgg ttgctagtaa atcgatagct ggttgcgctt atccatcaag cgctgcgagg
     1021 ccatttgtta tcaaacatca aacacccgta cgcagagcgc gcagagattg cgcaaaaggc
     1081 gcagaaggga aacgaaacat catctgcgtt gtctgcgttg tctgcgtagg gaggctccgg
     1141 catgctcagc gccggcgcag cgccgcacgc acggtgtcgg ccacgttctc gggcgtgaag
     1201 ccgaagtgct cgaacagcac cggcgccggc gccgactcgc cgaagcggtc aaggcccacc
     1261 accgcggcgc agccgtactt ccaccagccg tcggtggcac ccatctcgac ggcgatgcgc
     1321 ggcaggccgg cgggcagcac ggccttttgg tacttgagct cctggcggtc gaaggtggtg
     1381 gtcgagggca tcgacaccac gcgcaccgcg atgcggtgct cgcgggccag cagctcctgc
     1441 gccttcagcg ccagctgcac ctcggagccg gtggcgatca gcacggcctg cgccttgcgc
     1501 ttgaggccca ggtcggacgg ctcgctcagc acataggcgc cgcgcgagat gtcgcccagg
     1561 tcgcgcctgg ggcagtaggg caggttctgg cgcgacagca gcagcgccgt cggcagggtg
     1621 cgctgctgca gcgccaccgc ccaggcgacc acggtctcgg cggtatcggc cggacgccac
     1681 acgtccagcc ccggaatcag gcgcaggctg gcggcgtgct cgaccgcctg gtgtgtgggg
     1741 ccgtcctcgc ccaggccgat ggagtcgtgc g
//
LOCUS       DAOOOG010000347         1764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_86166_length_1764_cov_30.018139, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000347 DAOOOG010000000
VERSION     DAOOOG010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86166_length_1764_cov_30.018139"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PK242_14255"
     CDS             complement(<1..994)
                     /locus_tag="PK242_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOZ95231.1"
                     /translation="MSALHDLTVAEAARRLRARQLSALELARHLLARSQAHAGLGAFL
                     AQDEDVTLAQARAADARLAAGDAPPLTGVPVAHKDVFVTTDFPSTAGSRILAGYRSPF
                     ESTVTARLAEAGMVCLGKLNCDEFAMGSSNENSAFGPVRNPWDTTRVPGGSSGGSAAA
                     VAARLVPAATGSDTGGSIRQPSSFCGLTGIKPTYGRVSRYGMIAYASSFDQAGPMARS
                     AEDCALLLSSLCGPDLDRDSTSLELPPEDFTRQLGASIEGLRIGIPREFFGDGLAPDV
                     RAALDGALAELCKLGARLVDIRLPRTELSIPVYYILTPAEASTNLSRFDGVRYGH"
     gene            complement(991..1290)
                     /gene="gatC"
                     /locus_tag="PK242_14260"
     CDS             complement(991..1290)
                     /gene="gatC"
                     /locus_tag="PK242_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOZ95232.1"
                     /translation="MALTSDDIRHLAHLARLELNAEQSERMLTSLNSFFAIAQAMQAE
                     DTRGVAPLAHPLAAVQDVQLRLRDDVVSEPDRREAYQRNAPAVEDGLFLVPKVIE"
     gene            1491..>1764
                     /locus_tag="PK242_14265"
     CDS             1491..>1764
                     /locus_tag="PK242_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOZ95233.1"
                     /translation="MFGALRRYFSTDLAIDLGTANTLIYVRDKGIVLDEPSVVAIRHE
                     GGPQGKKTIQAVGREAKAMLGKVPGNIEAIRPMKDGVIADFTVTEQM"
BASE COUNT          273 a          602 c          637 g          252 t
ORIGIN      
        1 ggtggccgta gcgcacgccg tcgaagcggc tcaggttggt gctggcctcg gccggcgtga
       61 ggatgtagta gaccggaatc gacagctcgg tgcgcggcag gcggatgtcc accagccgcg
      121 cgcccagctt gcacagctcg gccagcgcgc catccagcgc ggcgcgcacg tcgggcgcca
      181 ggccgtcgcc gaagaactcg cgcggaatgc cgatgcgcag gccttcgatc gacgcgccca
      241 gctggcgcgt gaagtcctcg ggcggcagct cgagcgaggt cgagtcgcgg tccaggtcgg
      301 ggccgcacag gctggacagc agcagcgcgc agtcttcggc gctgcgcgcc atcgggccgg
      361 cctggtcgaa gctggaggcg taggcgatca tgccgtagcg cgacacccgc ccgtaggtgg
      421 gtttgatgcc ggtcaggcca caaaagctgg agggctggcg gatcgagccg ccggtgtcgc
      481 tgccggtggc cgccggcacc aggcgcgccg ccaccgccgc ggcgctgccg cccgaagaac
      541 cgcccggcac gcgcgtggtg tcccaggggt tgcgcaccgg accgaaggcc gagttctcgt
      601 tggacgagcc catggcgaat tcgtcgcagt tgagcttgcc caggcagacc atgccggcct
      661 cggccagccg cgccgtcacc gtgctctcga acggcgatcg atagccggcc aggatgcgcg
      721 aaccggcggt gctggggaag tcggtggtga cgaacacgtc cttgtgcgcc accggcacgc
      781 cggtgagcgg tggagcgtcg ccggccgcca gccgggcgtc ggccgcgcgc gcctgcgcca
      841 gggtcacgtc ctcgtcctgc gccagaaagg cgcccagccc ggcatgcgcc tggctgcgcg
      901 ccagcaggtg ccgggccagc tccagcgccg acagctggcg cgcgcgcagc cggcgggccg
      961 cctcggccac ggtcaggtcg tgcagcgcgc tcattcgatc accttcggca ccagaaacag
     1021 gccgtcctcc accgccggcg cgttgcgctg gtaggcctcg cgccggtcgg gctcgctcac
     1081 cacgtcgtcg cgcaggcgca gctgcacgtc ctggacggcg gccagcgggt gcgccagcgg
     1141 cgccacaccg cgggtgtcct cggcctgcat cgcctgggcg atggcaaaga acgagttcaa
     1201 gcttgtaagc atgcgctcac tttgttcggc attcagctcg agccgcgcca gatgggcgag
     1261 gtggcggatg tcatcagatg tcagggccat gaaaaaggca cgaaaaaaca gcaggagccc
     1321 ggctgccgcg gcgggtgcgc cgcaggggcc ggcgacgggg atgcgctatt atcccgcctt
     1381 tggcgcaacg gcccggaatt gggcgccatt tgcgcgaaat cacgctcatt tcgcattgat
     1441 tcagcggttg ccggcgtctg gtgctggccg gcggcccaga agggtttttc atgtttggag
     1501 ctctccgtcg gtacttctcc accgacctgg ccatcgacct cggcaccgcc aacaccctga
     1561 tctacgtgcg cgacaagggc atcgtgctgg acgagccgtc ggtggttgcg atccgtcacg
     1621 aaggtggccc gcagggcaag aagaccatcc aggccgtggg ccgcgaggcc aaggccatgc
     1681 tgggcaaggt gcccggcaac atcgaggcca tccgcccgat gaaggacggc gtgatcgccg
     1741 acttcaccgt caccgagcag atgc
//
LOCUS       DAOOOG010000348         1764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_86167_length_1764_cov_23.606788, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000348 DAOOOG010000000
VERSION     DAOOOG010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86167_length_1764_cov_23.606788"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1305
                     /locus_tag="PK242_14270"
     CDS             100..1305
                     /locus_tag="PK242_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOZ95234.1"
                     /translation="MSSTQQALIVGGGIGGLAAALAALRAGWEVRLFERAASFSEVGA
                     GVQLGPNVTRILHGWGLADELRAVAAFPHRLQVRCALTGAELGQLPLGQRAVERYGAP
                     YATIHRADIHGLLLHAVLRQGGAQLRLDEPLAGFTDDGKAVRLSTAGGKVVEGDALIG
                     ADGVRSRVRELLLGDGAPRPTGHLAYRTMLEQAALPHELRSAQVTAWLGPRLHLVQYP
                     VRGGAWMNVVAIVQGQPPAELDLWDHAAHAADLARAMGDTCAPLRELIRAANIATLNP
                     HPWRLWPLADRLPVGAAREMAQGLVALLGDAAHPMRPYLAQGAGMAIEDAAELGRALA
                     MEAVDVSTRLARYALARWQRVARVQAQSRRNGGIFHATGAVRLGRNLSMRLLGERLLD
                     QPWLYGTVS"
BASE COUNT          217 a          625 c          678 g          244 t
ORIGIN      
        1 caagcgctgg ggcctgatct ggcttgatgg ctcggatgct tcccacgacc tgatcgccgc
       61 ggccgtcgac tggccttcgc gcgcggcaca atcggcgcca tgagttcgac ccaacaagcc
      121 ctgatcgtcg gcggcggcat cggcggactg gcggcggcgc tggcggcgct gcgggccggc
      181 tgggaggtgc gcctgttcga gcgcgccgcc agtttcagcg aggtcggcgc gggggtgcag
      241 ctcggcccca acgtgacgcg catcctgcac ggctgggggc tggccgacga gctgcgcgcg
      301 gtggccgcgt ttccccatcg cctgcaggtg cgctgcgccc tcaccggcgc cgagctgggc
      361 cagctgccgc tcggtcagcg cgccgtcgaa cgctacggcg cgccctacgc caccatccac
      421 cgggccgaca tccacggcct gctgctgcac gccgtgctgc gccagggcgg cgcgcagctc
      481 aggctcgacg agccgctggc cgggttcacc gacgacggca aggcggtgcg cctgagcacc
      541 gccggcggca aggtggtgga aggcgacgcg ctgatcggcg ccgacggcgt gcgcagccgc
      601 gtgcgcgagc tgctgctggg tgacggcgcg ccgcgcccga ccgggcacct ggcctaccgc
      661 accatgctgg agcaggccgc gctgccgcac gagctgcgca gcgcccaggt cacggcctgg
      721 ctggggccgc gtctgcacct ggtgcaatac ccggtgcgcg gcggcgcctg gatgaacgtg
      781 gtggcgatcg tgcagggtca gccgccggcc gagcttgacc tgtgggacca cgccgcccat
      841 gcggccgacc tggcgcgcgc catgggcgac acctgcgcgc cgctgcgcga gctgatccgc
      901 gccgccaaca tcgccaccct gaacccgcat ccctggcggc tgtggccgct ggccgatcgc
      961 ctgccggtgg gcgccgcgcg cgagatggcg caaggcctgg tggcgctgct gggcgacgcc
     1021 gcccacccca tgcgacccta cctggcgcag ggcgccggca tggcgatcga agacgccgcc
     1081 gagctggggc gcgcgctggc gatggaagcg gtcgacgtgt ccacccggct ggcgcgctac
     1141 gcgctggcgc gctggcagcg cgtggcgcgc gtgcaggcgc agtcgcgccg caatggcggc
     1201 atcttccacg ccaccggggc ggtgcggctg gggcgcaacc tgtcgatgcg gctgctgggc
     1261 gagcggctgc tggatcagcc gtggctgtac ggcacggtca gctgaagggg acggcgcatc
     1321 attccgtcag cgttgttgac ctgggaatca gccggtggct tcggcctttg gatgtcttgc
     1381 ataccgttcg ggctgagctt gtccaggccc ttcaacaggc tcggggtgca cggttcggat
     1441 ttcaggcttg cgaagccata gggtgacacc ggctgcgccg gcaggggcca cgacccatgg
     1501 gtcatcgaag cggtccgctc aagtggtcag cctggccccg cgcttctgac gggggggccg
     1561 gatgccgccc gaccgagagc gcgccgccac gcggccagtc gaggcgccac gcaccgccgc
     1621 cgcccgggct gcggcgagca tcggccacgc cgggcgggtg tggcggtgtg gcccaggcgg
     1681 gcatcagggg gcgttgacgc cttcaggcca gtttggcggc gaactggacc agccggcgcg
     1741 cctggtgacc gatggcggcc ttga
//
LOCUS       DAOOOG010000349         1761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_86380_length_1761_cov_23.179367, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000349 DAOOOG010000000
VERSION     DAOOOG010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86380_length_1761_cov_23.179367"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..286
                     /locus_tag="PK242_14275"
     CDS             35..286
                     /locus_tag="PK242_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011882496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HOZ95235.1"
                     /translation="MAKKFPLKPAHPERICWGCDKYCPAGSMACGNGSDRTPHPAELF
                     GDDWHEWEPPVEGGASVAGTRDAEDPSRDAPDPGGSGQA"
     gene            416..1561
                     /locus_tag="PK242_14280"
     CDS             416..1561
                     /locus_tag="PK242_14280"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOZ95236.1"
                     /translation="MSFIATPQTMHFEAPLPLQSGASIGGYDLTYETYGQLNAERSNA
                     VLICHALNASHHVAGVYEGQARSEGWWDNMIGPGKPVDTERFFVIGINNLGSCFGSTG
                     PMHANPASGQVYGADFPVVTVEDWVNAQARLLDGLGIGQLAAVMGGSLGGMQALGWTL
                     QYPERVRHAVVVASAPNLSAENIAFNEVARRAIVTDPDFHGGHFYRHGVVPARGLRIA
                     RMIGHITYLSDDVMNEKFGRELRQAVAGNGSGYRYSTQDVEFQIESYLRHQGDKFSEY
                     FDANTYLLITRALDYFDPARAVGGDLTAALAHTRARFLLVSFSTDWRFSPARSREIVR
                     ALLENRRDVSYAEIDAPHGHDAFLLEDPRYLGVVRSYFERIARELHA"
     gene            1558..>1761
                     /locus_tag="PK242_14285"
     CDS             1558..>1761
                     /locus_tag="PK242_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOZ95237.1"
                     /translation="MSDEPIRRVIAGLVPQGSHVLDLGCGDGALLAHLARQRGCTGYG
                     IEIDDDNVLACVQRGVNVLQLNLH"
BASE COUNT          288 a          622 c          587 g          264 t
ORIGIN      
        1 ctgggataat tgagccgggt tctgccgcac cgccatggcg aagaaattcc ctctgaagcc
       61 agcgcacccc gagcgcatct gctggggttg cgacaagtat tgcccggccg gctcgatggc
      121 ctgcggcaac ggctccgacc gcacgccgca cccggcggag ctgttcggcg acgactggca
      181 cgagtgggag ccgccggtgg agggcggcgc ctcggttgcc ggcacgcgcg acgccgaaga
      241 ccccagccgt gacgctcccg accccggcgg cagtggccag gcatgacgcc gaccgctata
      301 ctgcacgcca cagcgccgat ttgcctctgc ttgcgggcgc tctacaaata ccgctaaatc
      361 cgagacacgc gtgcaacccg gtttcgattt ggcgaagccg ggtttttttg ttgccatgtc
      421 cttcattgcc acgccgcaga ccatgcactt cgaggcgccg ctgccgctgc aaagcggcgc
      481 gtcgatcggc ggctacgacc tgacctacga gacctacggc cagctcaacg ccgagcgcag
      541 caacgccgtg ctgatttgcc atgcgctcaa cgcctcgcac cacgtggcgg gggtgtacga
      601 aggccaggcg cgcagcgaag gctggtggga caacatgatc ggccccggca agccggtgga
      661 taccgagcgc ttcttcgtca tcggcatcaa caacctgggc tcctgcttcg gctccaccgg
      721 gccgatgcac gccaaccccg ccagcgggca ggtgtacggc gccgactttc cggtggtgac
      781 ggtggaggac tgggtcaacg cccaggcgcg actgctggac ggcctgggca tcgggcagct
      841 ggccgccgtg atgggcggca gcctgggcgg catgcaggca ctcggctgga cgctgcaata
      901 ccccgaacgg gtgcgccacg ccgtggtggt ggccagcgcg cccaacctgt cggccgagaa
      961 catcgccttc aacgaggtgg cgcggcgcgc catcgtcacc gaccccgact tccacggcgg
     1021 ccacttctac cgccacggcg tggtgccggc gcgcgggctg cgcatcgcgc gcatgatcgg
     1081 ccacatcacc tacctgagcg acgacgtcat gaacgagaag ttcggccgcg agctgaggca
     1141 ggcggtggcc ggcaacggca gcggctaccg ctactcgacg caggatgtcg agttccagat
     1201 cgaaagctac ctgcgccacc agggcgacaa gttcagcgag tatttcgacg ccaacaccta
     1261 cctgctgatc acccgcgcgc tcgactactt cgacccggcg cgcgccgtcg gcggcgacct
     1321 gacggcggcg ctggcgcaca cgagggcgcg ctttctgctg gtcagcttca gcaccgactg
     1381 gcgtttttcg cctgcgcgct cgcgcgagat cgtgcgcgcg ctgctggaaa accggcgcga
     1441 cgtcagctac gccgagatcg acgccccgca cgggcacgac gccttcctgc tggaagaccc
     1501 gcgctacctg ggcgtggtgc gctcctactt cgagcgcatc gcgcgggagc tgcacgcatg
     1561 agcgacgaac ccatccgccg cgtcatcgcc ggcctggtgc cgcagggctc gcacgtgctc
     1621 gacctgggct gcggcgacgg cgcgctgctg gcgcacctgg cgcgccagcg cggctgcacc
     1681 ggctacggca tcgagattga cgacgacaac gtgctggcct gcgtgcagcg cggcgtcaac
     1741 gtgctgcagc tcaacctgca c
//
LOCUS       DAOOOG010000350         1726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_89099_length_1726_cov_40.515859, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000350 DAOOOG010000000
VERSION     DAOOOG010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89099_length_1726_cov_40.515859"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1005
                     /gene="rfbD"
                     /locus_tag="PK242_14290"
     CDS             97..1005
                     /gene="rfbD"
                     /locus_tag="PK242_14290"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOZ95238.1"
                     /translation="MKILLLGKNGQLGWELQRSLAPLGELVALDRRGAPGLCGDLGEP
                     DGLAATVRQLCPDVIVNAAAYTAVDRAESEPELARRINAHAPAVLAREAAACGALLLH
                     YSTDYVFHGQGQRPWAEDDPTGPLNVYGQTKLEGEQAILRSAGRHLILRTSWVYAARG
                     GNFARTMLRLARQQPRLTVIDDQWGAPTGAELIADVSAHAIVQLRARPDKAGIYHLAA
                     AGETNWFSYAKHVIAQAQSAQAAIEYAVKDILPIPSSAYPVAATRPHNSRLDTRKLRT
                     AFDLHLPPWQAGVDRMLAEILGPSHA"
     gene            1002..>1726
                     /gene="rfbA"
                     /locus_tag="PK242_14295"
     CDS             1002..>1726
                     /gene="rfbA"
                     /locus_tag="PK242_14295"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOZ95239.1"
                     /translation="MNARKGIILAGGSGTRLHPATLAMSKQLLPVYDKPMIYYPLSTL
                     MLAGMRDILVISTPQDTPRFAQLLGDGSQWGITIQYAVQPSPDGLAQAFLIGESFIGG
                     APSALVLGDNLFYGHDFELLLSNAHARSEGATVFAYHVHDPERYGVVAFDAAGRATSI
                     EEKPAQPKSHYAVTGLYFYDAQVVDIAKTIRPSARGELEITTVNETYLKQGQLSVQIM
                     QRGYAWLDTGTHDSLLDAGQFIAT"
BASE COUNT          294 a          665 c          534 g          233 t
ORIGIN      
        1 gtggtacctg gaccaccccg actgggtggc gcaggtgcag tcgggcgcct accgcgactg
       61 ggtggccacg caatacggcg ctgccgccgc ccgcgcatga agatcctgct gctgggcaag
      121 aacggccagc tcggctggga gctgcagcgc agcctggcgc cgctgggcga gctggtggcg
      181 ctggatcgcc gcggcgcgcc cggcctgtgc ggcgacctgg gcgagcccga cggcctggcc
      241 gccacggtgc gccagctttg ccccgacgtc atcgtcaacg ccgccgccta caccgccgtc
      301 gaccgcgccg aaagcgagcc cgaactggcg cgccgcatca acgcccacgc gccggccgtg
      361 ctggcgcgcg aggccgccgc ctgcggcgcg ctgctgctgc actacagcac cgactatgtg
      421 tttcacggcc agggccagcg gccctgggcc gaggacgacc ccaccggccc gctgaatgtc
      481 tacggccaga ccaagctgga gggcgagcag gccatcctgc gcagcgccgg tcgccacctg
      541 atcctgcgca ccagctgggt ctacgcggcg cgcggcggca actttgcccg caccatgctg
      601 cgcctggcgc gccagcagcc gcgcctgacc gtgatcgacg accagtgggg cgcgcccacc
      661 ggcgccgagc tgatcgccga cgtcagcgcg cacgccatcg tgcagctgcg cgcgcggccc
      721 gacaaggccg gcatctacca cctggcggcg gccggcgaga ccaactggtt ttcctatgca
      781 aaacacgtga ttgcgcaggc ccaatctgcg caagcagcta tcgaatatgc agtaaaggac
      841 atcctcccga ttcccagcag cgcctacccc gtggcggcca cgcgcccgca caattcgcgc
      901 ctggacacgc gcaagctgcg caccgccttc gatctgcacc tgccaccctg gcaggccggc
      961 gtggaccgca tgctggccga aatccttgga ccttcccacg catgaacgca cgcaagggca
     1021 tcatcctggc cggcggctcc ggcacccgcc tgcacccggc caccctggcc atgagcaagc
     1081 agctgctgcc ggtgtacgac aagccgatga tctactaccc gctgtccacg ctgatgctgg
     1141 ccggcatgcg cgacatcctg gtcatcagca caccgcagga cacgccacgc tttgcgcaac
     1201 tgctgggcga tggcagccag tggggcatca ctatccagta cgccgtgcag cccagccccg
     1261 acgggctggc ccaggccttt ctgatcggtg aatccttcat cggcggcgcc cccagcgccc
     1321 tggtgctggg cgacaacctg ttctacggcc acgacttcga gctgctgctc tccaacgccc
     1381 atgcgcgcag cgaaggcgcc accgtgttcg cctaccacgt gcacgacccc gagcgctacg
     1441 gcgtggtcgc cttcgacgcc gccggccgcg ccaccagcat cgaagaaaaa cccgcgcagc
     1501 ccaaaagcca ctacgccgtc accggtctgt acttctacga cgcgcaggtg gtcgacatcg
     1561 ccaagaccat ccgacccagc gcgcgcggcg agctggaaat caccaccgtc aacgaaacct
     1621 acctcaagca gggccagctc agcgtgcaga tcatgcagcg cggctacgcc tggctggaca
     1681 ccggcaccca cgacagcctg ctcgatgccg gccagttcat cgccac
//
LOCUS       DAOOOG010000351         1724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_89242_length_1724_cov_25.064110, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000351 DAOOOG010000000
VERSION     DAOOOG010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89242_length_1724_cov_25.064110"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PK242_14300"
     CDS             <1..588
                     /locus_tag="PK242_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOZ95240.1"
                     /translation="LATHPASGLKNVADIVAQVKASKKLSYASFGNGTSAHLAGEMLK
                     SQLGVDIVHVPYKGQAPALADLLGGQVSMMFGNWPEFRNHVQSGKLVAIGMATAQRST
                     YAPDIPTLAEQGLKIESNSWNGLLAPAALPDAIVQRMNAAVNEAMKQPAVVEAFHKGG
                     IAARSGTPQQFADFIASETARYAEVIRRAGISADG"
     gene            669..1610
                     /locus_tag="PK242_14305"
     CDS             669..1610
                     /locus_tag="PK242_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOZ95241.1"
                     /translation="MSAGKLTAAVVQASSVLLDTPATVERALGLMAEAARQGAQLVVF
                     PEAFIGGYPKGADFHIYLGARTPQGRAEYKLYYDAAVSVDGPELAQLAEACARHQMYA
                     VFGIIEREGGTLYCTAVYLGPQGRVIGKHRKLMPTALERLVWGFGDGSTLQAVDTPWG
                     RLGAVICWENYMPALRLAMYQQRVTIYCAPTADDRDSWQSTMQHIALEGRCFVLSACQ
                     HLRRKDFPSERMNNRLPEAPDTVLMRGGSSIHDPLGKLLAGPVFGEDALLTAELDLSI
                     IPAAQMDFDPVGHYARPDVFSLHVNTAPQRAVQLGEQ"
     gene            1610..>1724
                     /locus_tag="PK242_14310"
     CDS             1610..>1724
                     /locus_tag="PK242_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HOZ95242.1"
                     /translation="MGQLALAAKITHVPSMYLSELPGARQGTRQDAIDGHKE"
BASE COUNT          298 a          624 c          568 g          234 t
ORIGIN      
        1 ctggccacgc acccggccag cggcttgaag aacgtggccg acatcgtggc ccaggtcaag
       61 gcgagcaaga agctcagcta cgcctcgttt ggcaacggta cctcggcaca tctggcgggc
      121 gaaatgctca agagccagct gggcgtcgac atcgtgcacg tgccctacaa gggccaggcc
      181 ccggcgctgg ccgacctgct gggcggccag gtgtcgatga tgttcggcaa ctggcccgag
      241 ttccgcaacc acgtgcagtc gggcaagctg gtggccatcg gcatggccac ggcccagcgc
      301 tcgacctacg cgccggacat ccccacgctg gccgagcagg ggctgaagat cgaatccaac
      361 tcctggaatg gcctgctggc gccggccgcc ttgccggacg ccatcgttca gcgcatgaac
      421 gccgccgtca acgaggccat gaagcagccc gccgtcgtcg aggcgttcca caagggtggc
      481 atcgccgccc gttccggcac gccgcagcag tttgccgact tcatcgccag cgaaaccgcg
      541 cgctacgccg aggtgatccg gcgcgccggc atcagcgccg acggctgaca cgccgcctcg
      601 cacaccccca ccacacaaaa ggagacaagc atgaccccca tcgccacccc gcagcgcagg
      661 agcgcgccat gagcgcgggc aagctgaccg ccgccgtggt gcaggcctcc tcggtgctgc
      721 tcgatacgcc ggccaccgtc gagcgcgcac tgggcctgat ggccgaggct gcgcgccagg
      781 gtgcgcagct ggtggtgttt cccgaagcct tcattggcgg ctaccccaag ggagcggact
      841 ttcacatcta cctgggcgcg cgcacgccgc aggggcgcgc cgagtacaag ctctactacg
      901 acgccgccgt cagcgtcgac ggtcccgaac tggcgcaact ggccgaagcc tgcgcgcgcc
      961 accagatgta cgccgtgttc ggcatcatcg agcgcgaagg cggcacgctg tactgcaccg
     1021 cggtctacct ggggccgcag gggcgcgtca tcggcaagca ccgcaagctg atgccgacgg
     1081 cgctggagcg cctggtgtgg ggcttcggcg acggctccac cctgcaggcg gtggacacgc
     1141 cctggggcag gctgggcgcc gtgatctgct gggaaaacta catgcccgcg ctgcgcctgg
     1201 ccatgtacca gcagcgcgtg acgatctact gcgcgcccac cgccgacgac cgcgactcgt
     1261 ggcagtccac catgcagcac atcgcgctgg aagggcgctg cttcgtgctg tcggcctgcc
     1321 agcacctgcg gcgcaaggac ttcccgtccg aacgcatgaa caaccggctg cccgaagcgc
     1381 ccgacaccgt gctgatgcgt ggcggcagca gcatccacga cccgctgggc aagctgctgg
     1441 ccggcccggt gttcggcgag gacgcgctgc tgacggccga gctggacctg tcgatcatcc
     1501 cggccgcgca gatggacttc gacccggtcg gccactacgc ccggccggac gtgttttcgc
     1561 ttcatgtcaa cacggcgccg cagcgcgccg tgcaactcgg agagcagtga tgggacaact
     1621 cgccctcgcc gccaagatca cccacgtgcc cagcatgtac ctgagcgagc tgcccggcgc
     1681 gcgccagggc acgcgccagg acgccatcga cggccacaag gaaa
//
LOCUS       DAOOOG010000352         1712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_90201_length_1712_cov_40.992758, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000352 DAOOOG010000000
VERSION     DAOOOG010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90201_length_1712_cov_40.992758"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PK242_14315"
     CDS             complement(<1..798)
                     /locus_tag="PK242_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOZ95243.1"
                     /translation="MQLHRAIFKAYDIRGTTPTTLNEAVAERLGLAFGQRALALGERV
                     VAVGRDGRLSGPALAAALMRGLVAAGVEVIDIGLATTPMLYFAAHTLCSSGIQVTGSH
                     NPRDDNGFKMVLAGRAIYGDEIQALRRAMEAGDGERRAGGSVRQHDVFPAYLARIQGD
                     IRLARPMKIVVDCGNGVAGASAPQVLRAIGCEVIELFTEVDGRFPNHHPDPSKPDNLR
                     DVIAALRASDTELGLAFDGDGDRLGIVTRDGQNIFPDRQLVLFAQDVL"
     gene            968..>1712
                     /locus_tag="PK242_14320"
     CDS             968..>1712
                     /locus_tag="PK242_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ95244.1"
                     /translation="MTALAAARPATSPARLTLYYLLLAATWGASFLFMRQATLEFGPL
                     PTAAVRVTVATLFLLPLLLAHGQWPALRRHWRPVLLCGLLNSGIPFALFSFALLHIST
                     GLSSILNATVPLFGALVAWAWLGQRPGISRGIGLVVGFVGVALLAWDKIGLRAGAPAA
                     LAAWAVAACLGATLCYALAASFTHRHLSGLPPLMTATGSQIGAMLGLALPALALWPAQ
                     MPGPTAWASVVALGVLCTGVAYVLYFRLIE"
BASE COUNT          198 a          621 c          612 g          281 t
ORIGIN      
        1 cagcacgtcc tgcgcgaaca gcaccagctg gcggtcgggg aagatgttct ggccgtcgcg
       61 ggtgacgatg cccaggcggt cgccgtcgcc gtcgaaggcc aggcccagtt cggtgtcgga
      121 cgcgcgcagc gcggcgatga cgtcgcgcag gttgtcgggc ttgctggggt cggggtggtg
      181 gttcggaaag cggccgtcga cctcggtgaa cagctcgatc acctcgcagc cgatggcgcg
      241 cagcacctgc ggcgcgctgg cgccggccac gccgttgccg cagtcgacca cgatcttcat
      301 cggccgcgcc agccggatgt cgccctggat gcgcgccagg tacgccggga acacgtcgtg
      361 ctggcgcacc gagccgccgg cgcggcgctc gccgtcgccg gcctccatcg cgcggcgcag
      421 ggcctggatt tcgtcgccat agatggcgcg gccggccagc accatcttga agccgttgtc
      481 gtcgcgcggg ttgtggctgc ccgtcacctg gatgccgctg ctgcacagcg tgtgcgcggc
      541 aaagtacagc atcggcgtgg tggccaggcc gatgtcgatc acctcgacgc cggcagccac
      601 caggccgcgc atcagcgccg ccgccagcgc cggaccggac aggcggccgt cgcgccccac
      661 cgccaccacg cgctcgccca gcgccagcgc gcgctgaccg aaggccaggc ccagacgctc
      721 ggcgacggcc tcgttcagcg tggtcggcgt ggtgccgcgg atgtcgtaag ccttgaagat
      781 ggcgcgatgg agctgcatgg gcggtgattg tagggagccg cacatgctat tgattcaata
      841 gctgccggcg cttgactgta ctgcgccaga gtccgatttc attccagatc cgtagcccag
      901 ccgccaggcc gaccgccttg ccccgcgccc ggcgccgggt ttggcccagc ggcgatactc
      961 gcgcgccatg accgccctcg ccgccgcccg cccggccacc agccccgccc ggctgacgct
     1021 gtactacctg ctgctggccg ccacctgggg ggcctcgttc ctgttcatgc gccaggccac
     1081 gctcgaattc ggaccgctgc cgaccgccgc ggtgcgcgtg acggtggcga cgctgtttct
     1141 gctgccgctg ctgctggcgc atggccagtg gccggcgctg cggcgccact ggcgcccggt
     1201 gctgctgtgc gggctgctca attccggcat tccgtttgcg ctgttcagct ttgcgctgct
     1261 gcacatctcc accggcctgt cgtcgatcct caacgccacc gtgccactgt tcggcgccct
     1321 ggtggcctgg gcctggctgg ggcaacggcc cggcatctcg cgcggcatcg gactggtggt
     1381 gggttttgtc ggcgtggccc tgctggcctg ggacaagatc ggcctgcgcg ccggcgcacc
     1441 ggcggcactg gccgcctggg ccgtggccgc ctgcctgggc gccacgctgt gttatgcgct
     1501 ggcggccagc ttcacgcacc gccacctgag cggcctgccg ccgctgatga ccgccaccgg
     1561 cagccagatc ggcgccatgc tgggcctggc gttgccggcg ctggcgctgt ggccggcgca
     1621 gatgcccggg cccacggcct gggcctcggt ggtggcgctg ggcgtgctgt gcaccggcgt
     1681 ggcctacgtg ctgtactttc gcctgatcga gg
//
LOCUS       DAOOOG010000353         1703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_90918_length_1703_cov_28.025485, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000353 DAOOOG010000000
VERSION     DAOOOG010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90918_length_1703_cov_28.025485"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="PK242_14325"
     CDS             complement(<1..145)
                     /locus_tag="PK242_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOZ95245.1"
                     /translation="MAVWTLGLNHHTAPLDLRGRFAFALDQLAPALKGLRASHARHPE
                     AAIL"
     gene            218..493
                     /locus_tag="PK242_14330"
     CDS             218..493
                     /locus_tag="PK242_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95246.1"
                     /translation="MDLLDLFWHGLGFVAPALVLAPAMVGVNRWLDRKRAPALSWQAQ
                     TAINFVACLAVLVAGLVLTGHDGRMATYAALVAVAAATPWALGAWRR"
     gene            complement(516..1265)
                     /gene="ugpQ"
                     /locus_tag="PK242_14335"
     CDS             complement(516..1265)
                     /gene="ugpQ"
                     /locus_tag="PK242_14335"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOZ95247.1"
                     /translation="MNARKLPAWPYPFWIAHRGAGRLAPENTLAAFRLGAQHGWRMFE
                     CDAKLSSDGVVFLLHDATLERTTDGAGSAGARPWGELAQLDAGSWLGRAWAGEPIPTL
                     ASVARFCLRNGYHLDIEIKPTPGLEAATGHAVAALAARLWQGAAAPPLLTSFQPAALA
                     AAAQTAPQLPRGLLLDALRDGWLEQARQLGCVAVVCQHALWDAGTVGQVRAAGLRALS
                     YTVNDEWAAERLIRLGTDGVVTDRVDLFSPA"
     gene            complement(1364..>1703)
                     /locus_tag="PK242_14340"
     CDS             complement(1364..>1703)
                     /locus_tag="PK242_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOZ95248.1"
                     /translation="PASTFVASFIGAPPMNLLKGVEGVRDGVLLGVRPEHLRVVDSGG
                     WTLHVETVEMLGAERLIYGHLNGPQGPWTVLRMNETEQPPAPGQQVQVLPLQDRLHGF
                     DEATGLRLDH"
BASE COUNT          227 a          565 c          640 g          271 t
ORIGIN      
        1 acaggatggc ggcttcgggg tggcgcgcgt gcgaggcgcg caggcccttg agcgcgggcg
       61 caagctggtc gagcgcgaac gcaaaacgcc cgcgcagatc cagcggcgcg gtgtgatggt
      121 tcagtcccag ggtccagacg gccatggcac cggattataa aattcggtgt tgttttcggc
      181 tggcctttgg ctgcagcgct tgactcggat caagaacttg gacctgctcg accttttctg
      241 gcatgggctc ggctttgtgg cccctgccct ggtgctggcg ccggccatgg tcggggtgaa
      301 tcggtggctg gatagaaaac gggctccggc gctttcctgg caagcgcaga cagctatcaa
      361 ttttgtggct tgtctggcgg tgctggtggc gggtctggtg ctgacgggcc acgatgggcg
      421 catggccacc tacgcggccc tggtggcggt tgccgccgcc acgccgtggg cgctgggcgc
      481 ctggcggcgc tgagcctgct ggcgcgggcg ccggctcagg ccgggctgaa cagatcgacg
      541 cggtcggtca ccacgccatc ggtgccgagc cggatcagcc gctcggcggc ccactcatcg
      601 ttgacggtgt agctgagcgc acgcaggccg gcggcccgga cctggccgac ggtgccggcg
      661 tcccacagcg cgtgctggca caccacggcc acgcagccga gctggcgcgc ttgctccagc
      721 caaccgtcgc gcagcgcatc cagcagcaga ccgcgcggca gctgcggcgc cgtctgcgcg
      781 gcggcggcca gggcggcggg ctggaacgag gtcagcagcg gcggtgccgc cgccccctgc
      841 cacaggcgcg cggccagcgc cgccacggcg tggccggtgg ctgcttccag gcccggggtg
      901 ggcttgattt caatgtccag gtggtagccg ttgcgcagac agaagcgcgc cacgctggcg
      961 agggtgggaa tgggctcgcc ggcccaggcg cgccccagcc agctgccggc gtcgagctgc
     1021 gccagctcgc cccagggccg cgcgccggcg ctgccggcgc cgtcggtggt gcgctccagc
     1081 gtggcgtcgt gcagcagaaa caccacgccg tcgctggaca gcttggcgtc gcattcgaac
     1141 atgcgccagc cgtgctgcgc gcccaggcgg aaggcggcca gggtgttctc gggcgccagc
     1201 cggccggcgc cgcggtgcgc gatccagaac gggtagggcc aggcggggag cttgcgcgca
     1261 ttcatggtca aaaatgcctg cagcgcttgc tggtcaagcg ccaacagcta ttgttttgat
     1321 agtttgcctt gagtctgggc acgaggggct ggttcacacc ggctcagtgg tccaggcgca
     1381 gcccggtggc ttcgtcgaag ccgtgcaggc ggtcttgcag cggcagcacc tgcacctgct
     1441 ggccgggtgc gggcggctgc tcggtttcgt tcatgcgcag cacggtccag gggccctgcg
     1501 ggccgttcag gtggccgtag atcaggcgct cggcgcccag catttcgacg gtttccacgt
     1561 gcagcgtcca gccgccgctg tcgaccacgc gcaggtgctc gggtcgcacg cccagcagca
     1621 ccccatcgcg cacgccttcg acgcccttga gcaggttcat gggcggcgcg ccgatgaagc
     1681 tggccacgaa ggtgctggcc ggg
//
LOCUS       DAOOOG010000354         1695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_91524_length_1695_cov_47.915854, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000354 DAOOOG010000000
VERSION     DAOOOG010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91524_length_1695_cov_47.915854"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1630
                     /locus_tag="PK242_14345"
     CDS             92..1630
                     /locus_tag="PK242_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010792189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOZ95249.1"
                     /translation="MKSKPRTPFLRATVTGLTPGRARPLVLALSCVLLTACASVGPDY
                     REPPPVDVGSGWSLPLASESQSADLSQWWSALDDPILDRLMATALAQNLDLRQAAARI
                     DEARALRDRVAGEALPTAAAGASVNRRRQSENGPLPVGSIPGLDATQTIYDAGFDAAW
                     EADLFGAKRRALEGASARLQATEAEAQGIRMRIVAEVARTWFTAVGARYELHAQQATL
                     DTLQQTLELVRLRHALGDASAADVEAAYAQWTAVNALIPDIQARQRAAILSLGVLLGA
                     PPERELALLDGPLTPSTLRALPVGERAEMLRRRPDVLAAERRLAASSADIGVATAELF
                     PKLSIGVGGGFQALSTGDWFDASSSRFSILPLISWRLFDGGRVRAEIRAREAAERQAA
                     LAYEQAVLTALGDAERALGDYHGGLDTLERRGMALDAARTSYGHAKARYAAGDIALVE
                     LLAAQRSLHEAETAAARAHTNAAVQLVALYKALGGGWDVSTTASTATHPLRGAAAPVA
                     FSSR"
BASE COUNT          259 a          600 c          579 g          257 t
ORIGIN      
        1 ccggtgacat tctcgccagc ctgtggccgc tggcggtcat cgccctcgcc acgctgacca
       61 tggccaccgt attcgtccga ggacgcctgc aatgaaatcc aagccccgca ctccctttct
      121 tcgcgcaacc gtcaccggcc tgacccctgg gcgcgcgcgt ccgctcgtgc tggccctgtc
      181 ctgcgtgctg ctgactgcct gcgcaagcgt gggccccgac taccgcgagc cgccgccggt
      241 cgacgtcggc agcggctgga gcttgccgtt ggcaagcgaa tcccagtccg cagacttgag
      301 ccaatggtgg tctgcactgg acgatcccat cctcgatcgc ctgatggcca cggcgctggc
      361 acagaacctg gatctgcgcc aggctgcggc acgcatcgat gaggcacgcg ccctgcgcga
      421 tcgtgtggcc ggcgaggcat tgcccaccgc cgccgctggc gcgagcgtca accggcgccg
      481 tcaaagcgag aacggacccc tgcccgtagg ttcgatcccc ggccttgacg ccacgcagac
      541 catctacgac gcgggcttcg acgcggcctg ggaggccgac ttgttcgggg ccaagcggcg
      601 ggcgctggaa ggcgccagcg cccgcctgca ggcgaccgaa gccgaggcgc agggcatacg
      661 catgcgcatc gtggccgagg tcgcgcgcac ctggttcacc gccgtcggcg cccgctacga
      721 gttgcacgca caacaggcca cactggatac gctgcagcag accttggaat tggtgcgcct
      781 gcggcatgcg ctgggcgacg cgtcggccgc cgacgtggag gcggcgtacg cccaatggac
      841 agcagtcaac gcgctcatcc cggatatcca ggcgcgtcag cgcgccgcga tactcagcct
      901 gggcgtgctg ctgggcgcgc cgccggagcg ggagctggca ctgctggatg gccccttgac
      961 gccgagcacg ctgcgggcgc tgccggtggg cgagcgcgca gagatgctgc gccggcgccc
     1021 ggacgtgctg gctgcggaac gtcgcctggc agcgagttcc gccgacatcg gcgtggccac
     1081 ggccgagttg ttccccaaac tctccatcgg tgtcggcggc gggttccaag cgctcagtac
     1141 gggcgattgg ttcgatgcat ccagctcgcg tttttccatc ctgccgctga tttcctggcg
     1201 cctgttcgac ggcggccgtg tgcgagccga aattcgggca cgcgaggcgg ccgagcggca
     1261 ggccgcgctg gcctatgagc aggcggtgct gacggcgctg ggcgacgccg agcgcgcgct
     1321 gggcgactac cacggcgggc tggacacact ggagcgccgc ggtatggcac tggatgccgc
     1381 gcgcaccagc tacggccacg ccaaggcgcg ctacgcggcg ggcgacattg cgctggtcga
     1441 actgctggcc gcccagcgca gcctgcacga ggccgaaacc gcagccgcgc gcgcacatac
     1501 caacgccgcc gtgcaattgg tggcgctgta caaggccctt ggtggtggct gggacgtatc
     1561 cacgacggca tcgaccgcaa cccatccgct gcggggcgct gccgcccccg tcgcgttttc
     1621 aagccgctga ttcaacacaa ggagaccgtc atgcaaatca acgttggaaa tctcgaccgc
     1681 attgtgcgca tcgtc
//
LOCUS       DAOOOG010000355         1684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_92391_length_1684_cov_21.246777, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000355 DAOOOG010000000
VERSION     DAOOOG010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92391_length_1684_cov_21.246777"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1091
                     /locus_tag="PK242_14350"
     CDS             81..1091
                     /locus_tag="PK242_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOZ95250.1"
                     /translation="METVQAALWVVVLLVALAIVFDFMNGFHDAANSIATVVSTGVLK
                     PTQAVVFAAFFNFIAIFIFHLSVAATVGKGIVQPGVVDTHVVFGALVGAISWNVLTWY
                     YGIPSSSSHALIGGIVGATVAKAGTGPLVMAGVLKTVAFIFVSPLLGYLLGSAMMVAV
                     AWVFRRVRPGRVDKWFRSLQLLSAGAYSLGHGGNDAQKTIGIIWMLLIATGYAGAGES
                     SPPLWSIVSCYAAIAAGTMFGGWRIVKTMGQKITKLKPVGGFCAESGGALTLFIATGL
                     GIPVSTTHTITGAIVGVGSTQRASAVRWGVAGNIVWAWVLTIPASALVAALAYWLSLL
                     LF"
     gene            complement(1163..1609)
                     /locus_tag="PK242_14355"
     CDS             complement(1163..1609)
                     /locus_tag="PK242_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95251.1"
                     /translation="MKNKTLATWLAFLGGPLGLHRFYLRGFGDLLGWLLPIPTALGLY
                     GIERVQQFGQDDPLSWLLIPLLGFTISACALTAIVYGLMSAEHWNARFNPQADPQAAP
                     GQTHWGTVFGISVALLIGAGVLMASLAFSFQRYFEYQIEEARKISQ"
BASE COUNT          253 a          551 c          589 g          291 t
ORIGIN      
        1 ccatcaccga caagtgcgag gacgtggcca accagatcga gggcatcgtc ctcgaaaact
       61 cctgagccgg gcgcgcagcc atggaaacgg tgcaggccgc gctctgggtc gtcgtgctgc
      121 tggtcgcgct ggcgatcgtg ttcgacttca tgaacggctt tcatgacgcc gccaactcga
      181 tcgccacggt ggtctcgacc ggcgtgctca agcccaccca ggcggtggtg tttgccgcct
      241 tcttcaactt catcgccatc ttcatcttcc acctcagcgt ggccgccacc gtgggcaagg
      301 gcatcgtgca gccgggggtg gtggacaccc acgtggtgtt cggcgccctg gtgggggcca
      361 tcagctggaa cgtgctgacc tggtactacg gcatccccag cagttcctcg cacgcgctga
      421 tcggtggcat cgtcggcgcc accgtggcca aggccggcac cggcccgctg gtgatggccg
      481 gggtgctcaa gacggtggcc ttcatcttcg tgtcaccgct gctgggctac ctgctgggct
      541 cggccatgat ggtggcggtg gcctgggtgt tccggcgtgt gcggccgggg cgggtcgaca
      601 aatggtttcg cagcctgcag ctgctgagcg ccggcgccta cagcctgggc cacggcggca
      661 acgacgccca gaagaccatc ggcatcatct ggatgctgct gatcgccacc ggctatgccg
      721 gcgccggtga atccagcccg ccgctgtggt ccatcgtgag ctgctacgcc gccatcgctg
      781 ccggcaccat gttcggtggc tggcgcatcg tcaagaccat ggggcagaag atcaccaagc
      841 tcaagccggt cgggggcttt tgcgccgaat cgggcggggc gctgaccctg ttcatcgcca
      901 ccgggctggg catcccggtg tcgaccacgc acaccatcac cggcgccatc gtgggggtcg
      961 gctccacgca gcgcgccagc gcggtgcgct ggggcgtggc cggcaacatc gtctgggcct
     1021 gggtgctgac cataccggcc agcgcgctgg tggcggcgct ggcctactgg ctgagcctgc
     1081 tgctgttctg aacgcgctat gtgttcaata gctggtggcg cagtctgggc gctattgagc
     1141 gcctgattgt tcgcttcacg cgctactgcg agatcttgcg cgcttcctcg atctggtact
     1201 cgaaatagcg ctgaaagctg aacgccagcg acgccatcag cacaccggcg ccgatcagca
     1261 gcgccaccga gatgccgaac accgtgcccc aatgggtctg gccgggcgcg gcctgcgggt
     1321 cggcctgggg gttgaagcgg gcgttccagt gctcggcgct catcaggccg tagacgatgg
     1381 cggtcagcgc gcaggccgag atggtgaagc ccagcagcgg gatcagcagc cagctcagag
     1441 ggtcgtcctg gccgaactgc tgcacgcgct cgatgccgta caggccgagc gcggtcggga
     1501 tcggcagcaa ccagcccagc aggtcgccaa agcccctcag atagaagcgg tgcaggccca
     1561 gggggccgcc gagaaaggcc agccaggtgg ccagggtctt gtttttcatg cggttcggca
     1621 aacggtgtcg gacgggcgcc cgcgccgtgc gggccggggt ttctgggcgc cgccggcggc
     1681 ggcc
//
LOCUS       DAOOOG010000356         1682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_92575_length_1682_cov_28.757222, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000356 DAOOOG010000000
VERSION     DAOOOG010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92575_length_1682_cov_28.757222"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PK242_14360"
     CDS             complement(<1..766)
                     /locus_tag="PK242_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HOZ95252.1"
                     /translation="MKRILIANRGEIARRVIATARRMGLETVAVHSDADANALHVREA
                     TLACALGGNSSADSYLRIDKLLAAARATGADAVHPGYGFLSEDAGFAQAVQDAGLTWI
                     GPPPAAIRALGSKAAAKALATKQGVPCLPGYAGEDQSEARFAAEATRIGYPVMVKAVS
                     GGGGRGMRLVTEAAQLPQALASARSEALAGFGNGELLIERAVLQPRHVEVQVFADAHG
                     HAIHLGERDCSVQRRHQKIIEEAPSPAVSPELRARMG"
     gene            complement(768..>1682)
                     /locus_tag="PK242_14365"
     CDS             complement(768..>1682)
                     /locus_tag="PK242_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOZ95253.1"
                     /translation="VQTDMCTPALARFGSDELKRQYLAPAIAGDMVGCIGVSEPGAGS
                     DVSGIKSTARKDGDDYVITGQKMWITNSLQADWMCMLVNTGGDNPHKNKSLILVPMRE
                     GPGGPLRKGISVARKIRKIGMHSSDTGLIYFDEVRVPQRNLIGAEGQGFIYQMQQFQE
                     ERLWAAANALSALDGCIRDTIEWARERKMFGATLLDQQWVQFKLVELQTEVEALRALT
                     WKAGERYVAGDDVLQWASMGKLKAGRLMREVADTCLQFWGGMGFASDSRVSRLYRDGR
                     LGSIGGGADEVMMGIIAKTMGMAKRPGA"
BASE COUNT          226 a          610 c          568 g          278 t
ORIGIN      
        1 cgcccatgcg cgcgcgcagc tcgggcgaga cggccgggct gggcgcttcc tcgatgatct
       61 tctgatggcg cctttgcacc gagcagtcgc gctcgcccag gtggatggca tggccgtgcg
      121 cgtcggcaaa cacctgcact tcgacgtggc gcggctgcag cacggcgcgc tcgatcaaca
      181 gctcgccatt gccaaagccg gccagcgctt ccgagcgcgc gctggccagc gcctgcggca
      241 gctgcgccgc ctcggtcacc agccgcatgc cgcgcccgcc gccaccggac acggccttga
      301 ccatcaccgg gtagccgatt ctggtggcct cggcggcaaa gcgcgcctcg gactggtcct
      361 cacccgcgta gccgggcagg cagggcacgc cctgcttggt cgccagcgcc ttggccgcgg
      421 ctttgctgcc cagggcgcgg atggcggcgg gcggcgggcc gatccaggtc aggccggcgt
      481 cctgcaccgc ctgggcaaaa ccggcgtctt cgctcagaaa gccatagccg gggtgcaccg
      541 cgtcggcgcc ggtggcgcgg gcggcggcca gcagcttgtc gatgcgcaga tagctgtcgg
      601 ccgagctgtt gccacccagc gcacaggcca gcgtggcttc gcgcacgtgc agggcattcg
      661 cgtcggcgtc ggagtgcacg gccacggtct ccaggcccat gcggcgcgcc gtggcgatca
      721 cacggcgcgc gatctcgccg cggttggcga tgaggatgcg tttcaagtca cgctcccggg
      781 cgtttcgcca tgcccatggt cttggcgatg atgcccatca tcacctcgtc ggcgccgccg
      841 ccgatggaac ccaggcggcc gtcgcggtac aggcgcgaga cccggctgtc actggcaaag
      901 cccatgccgc cccagaactg caggcaggtg tcggccacct cgcgcatcag ccgcccggcc
      961 ttgagcttgc ccatgctggc ccattgcagc acgtcgtcgc cggcgacgta gcgctcgccg
     1021 gccttccacg tgagcgcgcg cagcgcctcg acttcggtct gcagctcgac cagcttgaac
     1081 tgcacccact gctggtcgag caaggtggcg ccgaacatct tgcgctcgcg cgcccattcg
     1141 atggtgtcgc ggatgcaacc gtcgagcgcc gacagcgcat tggccgccgc ccacaggcgc
     1201 tcttcctgga actgctgcat ctggtagatg aagccctggc cctcggcgcc gatcaggttg
     1261 cgctgcggca cgcgcacctc gtcgaagtag atcaggccgg tgtcactgct gtgcatgccg
     1321 atcttgcgga tcttgcgcgc caccgagatg cccttgcgca gcgggccgcc ggggccctcg
     1381 cgcatgggca ccaggatcag gctcttgttc ttgtgcgggt tgtcgccgcc ggtgttcacc
     1441 agcatgcaca tccagtcggc ctgcaggctg ttggtgatcc acatcttctg gccggtgatg
     1501 acgtagtcgt cgccgtcctt gcgcgccgtg ctcttgatgc ccgacacgtc gctgccggcg
     1561 cccggctcgc tcacgccgat gcagcccacc atatcgccgg cgatggcggg cgccaggtac
     1621 tggcgcttga gctcgtcgct gccaaagcgc gccagcgccg gcgtgcacat gtcggtctgc
     1681 ac
//
LOCUS       DAOOOG010000357         1681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_92656_length_1681_cov_18.816728, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000357 DAOOOG010000000
VERSION     DAOOOG010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92656_length_1681_cov_18.816728"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1566)
                     /locus_tag="PK242_14370"
     CDS             complement(76..1566)
                     /locus_tag="PK242_14370"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ95254.1"
                     /translation="MVVDTPGGRFRVDYDEQLPISPVGPLVFFAQFLEASGHFEALCA
                     KAPLSYTSPNAPDKRAVLATMVLGILAGCRRFAHLEALRGDAVAAGLLGVEGLVCEDS
                     VRRALYRLDPTAAAQWLRGRYDAVVEELLAQPWVLDTDVTIKPLYGRQEGAEYGYNPK
                     KPGRPSHAYHTYWMARLRLCLDVEVRPGNEHGVNYGLDGLFAWLDARPRAQWPQFVRG
                     DCGYGQESVLARCEERNLGYLFKLRRTKNARALLARLESAAEAAWQPIGQGWEAAEGE
                     LKLDGWTRRRRVIALRRRLETAPGPRAERRRAKRLALPLWENCGVHATVEVLEYEYQL
                     LVTNLSHEPLTLAVLYRERGDAENPFDELKNQWGWAGFTSRKLRVSQISARLIALVYN
                     WWSLYARLIEPTQHHEAVRSRPRLLGGVARQSQHAGQRRLAVRLLHGDAPALRPCIER
                     AVSFLQTLLATAEQLDASERWRRILRHLFAAQMQSAGPEPPALACS"
BASE COUNT          261 a          616 c          559 g          245 t
ORIGIN      
        1 cctaaacgaa gcagttagga ggctaccgaa gcggcgagcg catcggtaaa cggcgggaat
       61 cgcgggcaga tcggctcagg aacaggccag cgcgggcggt tccgggccgg cggactgcat
      121 ctgggcggca aagaggtggc gcaagatccg tctccagcgt tcggaggcat ccaactgctc
      181 cgcagttgcc aagagcgttt gcaggaagct gacggcccgt tcgatgcagg ggcgcagcgc
      241 gggggcgtcg ccgtgcagca gtcgcacggc caggcggcgt tggccggcgt gctggctttg
      301 gcgggcgacc ccgccgagca ggcgcgggcg gctgcgcacg gcctcgtggt gctgcgtggg
      361 ctcgatgagg cgcgcgtaca ggctccacca gttgtagacc agggcgatga gccgggcgct
      421 gatctggctg acgcgcagct tgcgcgaggt gaagccggcc cagccccact ggttcttgag
      481 ctcgtcgaag gggttctccg cgtcgccgcg ctcacggtac agcacggcca gcgtcagcgg
      541 ttcgtgcgac aagttggtca ccagcaactg gtactcgtac tcgagcacct cgacggtggc
      601 gtgcaccccg cagttctccc acaacggcaa cgccaggcgt ttggcccgcc gccgctcggc
      661 ccgcggcccc ggcgcggttt ccagccggcg gcgcaacgcg atcacccgcc ggcgccgcgt
      721 ccagccgtcc aacttcagct cgccctcggc ggcctcccag ccctggccga tcggctgcca
      781 cgccgcctcg gcggccgact ccagccgcgc cagcagcgcg cgggcgttct tggtgcggcg
      841 caatttgaag aggtagccga ggttgcgttc ctcgcagcgc gccaggaccg actcctggcc
      901 gtaaccacaa tccccgcgca cgaactgcgg ccactgcgcc cgcggccgcg cgtcgagcca
      961 ggcgaacagc ccgtccagcc cgtagttgac gccatgctcg ttgcccggcc gcacctccac
     1021 gtccaggcac aggcgcaacc gcgccatcca ataggtgtgg taggcgtggc tgggccggcc
     1081 gggcttcttg gggttgtagc cgtactcggc cccctcctgc cgcccgtaca acggcttgat
     1141 cgtcacatcg gtatccaata cccaaggctg cgcgagcagc tcctcgacca ccgcatcgta
     1201 acgtccccgc agccattggg cggcggcggt cggatcgagc cggtagagcg cgcggcgcac
     1261 gctgtcctcg cacaccaacc cctccacgcc cagcagccca gcggcgaccg cgtcgccgcg
     1321 caacgcctcc agatgggcga agcgccggca cccggcgagg atgcccagca ccatcgtcgc
     1381 caacaccgcg cgtttgtccg gcgcgttcgg actggtgtag ctcagcggcg ctttggcgca
     1441 gagcgcctcg aagtgcccgc tggcctccag gaactgcgcg aagaacacca gcggccccac
     1501 cgggctgatc ggcagctgct cgtcgtagtc gacccggaat cgcccgccgg gcgtgtcgac
     1561 caccatgccc gccgagccct cacccgctgg gtgaaccgtc ttggccaatc cggaaggtgt
     1621 catccctcgc gagataaaca ccttccgccg tttcgcccag ctcccaactg ctccttatag
     1681 g
//
LOCUS       DAOOOG010000358         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_93024_length_1677_cov_29.663995, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000358 DAOOOG010000000
VERSION     DAOOOG010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93024_length_1677_cov_29.663995"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PK242_14375"
     CDS             <1..207
                     /locus_tag="PK242_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOZ95255.1"
                     /translation="CLYVTRATLFTHIATRERCQAMADELFAVVRSGQVRIHIDQRFA
                     LQDVQAAHRALEARQTTGSTILTL"
     gene            complement(290..688)
                     /locus_tag="PK242_14380"
     CDS             complement(290..688)
                     /locus_tag="PK242_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOZ95256.1"
                     /translation="MTPHPIDAWHALVRSHDPRGLDALLADDAVFISPVVHTPQRGKA
                     VTRAYLSAAFQVFVNPTFRYVREIRGPSDAMLEFETEIDGILVNGVDIIRWGADGRIA
                     EFKVMVRPLKAIQLIHQRMAALLAAAPRSA"
     gene            complement(1007..>1677)
                     /locus_tag="PK242_14385"
     CDS             complement(1007..>1677)
                     /locus_tag="PK242_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HOZ95257.1"
                     /translation="CNRYESPQAREIERLWHVGRDQPSRWWDEVLHPRGQGPFIRRAR
                     EGGGRELVVGQWGLIPWFAPQARLAYSTNNARSEELASKASYKQPWARGQRCIIPACS
                     FDEPCWETGRNVWWRFRRADGQPWGLAGLWNTWIDRASGEVHESYTMLTINADQHPLM
                     RRMHKPDPKLAPDQQDKRSVIAIEPGDVPQWLEGTLDEARALLRAPAPELFDAGPAVQ
                     LPAL"
BASE COUNT          261 a          598 c          556 g          262 t
ORIGIN      
        1 tgcctgtacg tcacgcgcgc caccctgttc acccacatcg ccacgcgcga gcgctgccag
       61 gccatggccg acgagctgtt tgccgtggtg cgctccggcc aggtcaggat ccacatcgac
      121 cagcgctttg ccctgcagga cgtgcaggcc gcccatcgcg ccctggaagc gcgccagacc
      181 accggatcga ccatcctgac gctgtaagcc ggcgcgcggc cgcctgggtt gcggcttgcg
      241 cgccaagggg cacaagcgcc ctggccccgg cgggcctgcg catgccaagt caggccgatc
      301 gcggggcggc ggccagcagg gccgccatgc gctggtggat caactggatc gccttcagcg
      361 ggcgcaccat caccttgaac tcggcaatgc ggccgtcggc gccccagcgg atgatgtcca
      421 cgccgttgac caggatgccg tcgatctcgg tctcgaactc cagcatcgcg tccgacgggc
      481 cgcgaatctc gcgcacgtag cggaacgtcg ggttgacgaa cacctgaaac gccgccgaca
      541 gataggcccg cgtgaccgcc ttgccccgct ggggcgtgtg caccaccggc gagatgaaca
      601 ccgcgtcatc ggccagcagc gcgtccagcc cgcgcgggtc gtgagagcgg acaagggcgt
      661 gccaggcgtc gatcgggtgt ggcgtcatgc ggcaatggta gcgcggcgcg cgcgcgcccg
      721 gacaagcccg ccaagccggc cgcccggccc gtcgcccgca gggtgagccc ggcctggagg
      781 cggggctggc gactgactga tgcggtcaat cactatctac tgagcactat cgaattggta
      841 gctgatcgcg cttgatgggt gaagggtgaa ggctaaaact ctcatgaaaa tcatgatttg
      901 accctacttc acctctctga tcccaattca ccccatcagc cccgcttgtc ttgtcaaaac
      961 aatagctgtt agcgtttgct ggacgggcgc agcaggcgaa acgcgctcat aaagcgggca
     1021 gctgcacggc cgggcccgcg tcaaacagct ccggcgccgg cgcgcgcagc agggcgcgcg
     1081 cctcgtccag cgtgccctcc agccactgcg gcacgtcgcc cggttcgatg gcaatcacgc
     1141 tgcgcttgtc ctgctggtcg ggcgccagct tggggtcggg cttgtgcatg cggcgcatca
     1201 gcgggtgctg gtcggcgttg atcgtcagca tggtgtagct ctcgtgcacc tcgccgctgg
     1261 cgcggtcgat ccaggtgttc cacagccctg ccagacccca gggctggccg tcggcgcgcc
     1321 gaaagcgcca ccacacgttg cgccccgtct cccagcacgg ctcgtcgaac gagcaggccg
     1381 ggatgatgca gcgctggccc cgcgcccagg gctgcttgta cgaggccttg ctcgccagct
     1441 cctcgctgcg cgcgttgttg gtgctgtacg ccaggcgcgc ctgcggcgca aaccacggaa
     1501 tcagccccca ctggcccacc accagctcgc gcccgccgcc ctcacgcgcg cggcgaatga
     1561 acggcccctg tccacgcggg tgcagcacct cgtcccacca gcggctgggc tgatcgcggc
     1621 ccacgtgcca gaggcgttcg atttcgcggg cttgggggga ctcgtagcgg ttgcaca
//
LOCUS       DAOOOG010000359         1661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_94456_length_1661_cov_27.303861, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000359 DAOOOG010000000
VERSION     DAOOOG010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94456_length_1661_cov_27.303861"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..743)
                     /locus_tag="PK242_14390"
     CDS             complement(18..743)
                     /locus_tag="PK242_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOZ95258.1"
                     /translation="MIGRLVLLLLAANLGWLAWTQGWLRPLGLVPQEQAEPQRLQRQV
                     QPQALRLQPLEPASAAAAADDTPARRSTAASATPASAAEPAAASASAPAPTACLQIGT
                     FDASQIEAVRQAAAGLPEGSWRVDAVQLPGRWMVYLGKLPDAAAVAARRAELRAQGVD
                     TDRPGAALEPGLSLGRFASEEAAERALDNLARKGVRGARVVRERRDTPAYLLRLPQAD
                     AALRRQARALNGALGGHDWRPCE"
     gene            complement(740..1540)
                     /locus_tag="PK242_14395"
     CDS             complement(740..1540)
                     /locus_tag="PK242_14395"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOZ95259.1"
                     /translation="MTAPTLSWPVDALRSALTPLLPGLVVEVAGQIDSTNSELMRRAR
                     AGRLPPTLLVAELQTAGRGRLGRGWQSGDGEAGGALAFSLALPLAPHDWSGLSLAVGL
                     AVAEALHPDIRLKWPNDLWWQGRKLAGILVETASGAAAAGAGRCAIVGIGVNIAPLDG
                     TGFATPPAWLQALLPGVDAPGALLRIAVPLVHALHAFEQTGFAPLAPRFATRDLLAGQ
                     PVQLSDGSEGVALGVGPDGALRVRCATGVREVRSAEVSVRPAAPGAPA"
BASE COUNT          184 a          625 c          673 g          179 t
ORIGIN      
        1 ttgtccaagc catcggttca ttcgcagggg cgccagtcgt gcccgccgag ggcgccgttg
       61 agggcgcgcg cctgccggcg cagcgcggcg tcggcctgcg gcaggcgcag caggtaggcc
      121 ggtgtgtcgc gccgctcgcg caccacgcgg gcgccgcgca cgcccttgcg ggccaggttg
      181 tcgagcgcgc gctcggccgc ctcttcgctg gcaaagcgcc ccagcgacag gccgggctcc
      241 agcgccgcgc cggggcggtc ggtgtccacg ccctgggcgc gcagctcggc gcggcgcgcg
      301 gccacggcgg cggcgtcggg cagcttgccg agatacacca tccagcggcc cggcagctgc
      361 accgcatcga cgcgccagct gccctcgggc aggccggcgg cggcctggcg cacggcctcg
      421 atctggctgg cgtcgaaggt gccgatctgc aggcaggcgg tgggcgcggg ggcgctggcg
      481 gatgcggcgg ccggttcggc ggcgctggcc ggggtggccg aggcggcggt gctgcggcgc
      541 gccggggtgt cgtcggccgc cgcggcggcc gaagccggct ccagcggctg caggcgcagg
      601 gcctggggct gcacctggcg ctgcaggcgt tgcggctcgg cctgctcttg cggcaccagc
      661 cccagcgggc gcagccagcc ctgggtccag gccagccagc ccaggttggc ggccagcagc
      721 agcagcacca gccggccgat catgcgggcg ccccaggcgc tgccgggcgc acgctgactt
      781 cggcgctgcg cacttcgcgc acgccggtgg cgcagcgcac gcgcagcgcg ccatcggggc
      841 cgacgcccag ggccacgcct tcgctgccgt cggacagctg caccggctgg ccggccagca
      901 ggtcgcgggt ggcaaagcgc ggcgccagcg gcgcaaagcc ggtctgctcg aaggcgtgca
      961 gcgcatgcac cagcggcacc gcgatgcgca gcagcgctcc cggggcgtcc accccgggca
     1021 gcagcgcctg cagccaggcc ggcggggtgg cgaagccggt gccgtcgagc ggcgcgatgt
     1081 tgaccccgat gccgacgatg gcgcagcgcc cggcgccggc ggccgctgcg ccgctggcgg
     1141 tttcgaccag gatgccggcc agcttgcggc cctgccacca caggtcgttg ggccatttga
     1201 ggcggatgtc ggggtgcagc gcctcggcca ccgccaggcc cacggccagc gacaggcccg
     1261 accagtcgtg cggcgccagc ggcagcgcca gcgaaaacgc cagcgcgccg cccgcttcgc
     1321 cgtcgcccga ctgccagccg cggcccagcc gcccgcggcc ggcggtctgc agctcggcca
     1381 ccagcagggt gggcggcagg cggccggcgc gggcgcggcg catcagctcg ctgttggtgg
     1441 agtcgatttg ccccgccact tcgaccacca ggcccggcag cagcggcgtc agcgcgctgc
     1501 gcagcgcgtc gaccggccag gacagggtgg gggcggtcat ggcggcggct gccggccgga
     1561 cttgcggccc gaccggcggc ggccggcctg gtcttcggcg ctgtcgaggt cggacgacag
     1621 cagggtgccg cggcagcgcc ggctgccgca gcggcagggg t
//
LOCUS       DAOOOG010000360         1656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_94933_length_1656_cov_31.908182, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000360 DAOOOG010000000
VERSION     DAOOOG010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94933_length_1656_cov_31.908182"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PK242_14400"
     CDS             <1..423
                     /locus_tag="PK242_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95260.1"
                     /translation="ELTRCAARVASLDFAPCTGFEPLRADAGEAERAYADYLAGQAGA
                     SQAALLPPPHRAVAAGSQAAAAIDEPLARLIAAGVLLRQGRASPALVARAVDTASAQG
                     WRRPLLAWLGVQARLAEQAGQTEEAARIRRRMDLVGPR"
     gene            complement(434..1393)
                     /gene="rsgA"
                     /locus_tag="PK242_14405"
     CDS             complement(434..1393)
                     /gene="rsgA"
                     /locus_tag="PK242_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOZ95261.1"
                     /translation="MSRPRQPPGPSADAQLLDGLVVGSHGRHCVVETPAGERVICHPR
                     GKKLAVVVGDGVRWRPSHDEGTVEQITPRRNLLYRQDAVRTKSFAANLDQVLILVAAE
                     PEFSEHQLARALIACEAAHIEPLIALNKADLAEPFARAWQRLAPYRAMGYAVLPLAAK
                     PRGPAPAQADTLAALGQRLHRRTTLILGPSGAGKSTLINRLIPGAAAQTGEISQALNS
                     GKHTTTTTTWYWLDEARASALIDSPGFQEFGLHHIDPMQLAAYMPDIRPHVAECRFYN
                     CTHLHEPGCGVTAQVQSSAGADGISASRYRIYSELFAELRDSR"
BASE COUNT          194 a          554 c          655 g          253 t
ORIGIN      
        1 gagctgacgc gctgcgccgc ccgggtggcc agcctggact tcgcgccctg caccggcttt
       61 gagccgctgc gtgccgacgc gggcgaggcc gagcgcgcct atgccgacta cctggccggc
      121 caggccggcg cgtcgcaggc cgcgctgctg ccgccgccgc accgggcggt cgccgcgggc
      181 agccaggccg cggccgccat cgacgagccg ctggcgcgcc tgatcgccgc cggcgtgctg
      241 ctgcgccagg gtcgcgccag cccggcgctg gtggcgcggg cggtggacac cgcgtccgcc
      301 cagggctggc gccgcccgct gctggcctgg ctgggcgtgc aggcacggct ggccgagcag
      361 gccggacaga ccgaggaagc cgcgcgcatc cgccgccgca tggacctggt ggggccgcgc
      421 tgaggcgccg ccatcaccgg ctgtcgcgca gttcggcaaa cagttcgctg taaatcctgt
      481 agcggctggc actgatcccg tctgcgccag cgctggattg cacctgggcc gtcacgccgc
      541 agccgggctc gtgcaggtgg gtgcagttgt agaaccggca ctcggccacg tgggggcgga
      601 tgtcgggcat gtaggcggcc agctgcatgg ggtcgatgtg gtgcaggccg aattcctgga
      661 agcccggtga gtcgatcagc gcgctggcgc gggcctcgtc cagccagtac caggtggtgg
      721 tggtggtggt gtgcttgccg gagttgagcg cctgcgagat ctcgccggtc tgcgccgcgg
      781 cgccggggat caggcggttg atcagcgtgc tcttgccggc gcccgaggga cccaggatca
      841 gcgtggtgcg ccggtgcagg cgctggccca gcgcggccag ggtgtcggcc tgggcgggcg
      901 ccgggccgcg cggcttggcg gccagcggca gcacggcgta gcccatggcg cggtagggcg
      961 ccaggcgctg ccaggcgcgg gcaaacggct cggccaggtc ggccttgttg agggcgatca
     1021 gcggctcgat gtgcgcggcc tcgcaggcga tcagggcgcg cgccagctgg tgctcggaaa
     1081 actccggctc ggccgccacc aggatcagca cctggtccaa gttggcggca aagctcttgg
     1141 tgcgcacggc gtcctgccga tacagcaggt tgcggcgcgg cgtgatctgc tcgaccgtgc
     1201 cttcgtcgtg cgaaggccgc cagcgcaccc cgtcgcccac caccacggcc agcttcttgc
     1261 cgcgcgggtg gcagatgacg cgctcaccgg cgggcgtttc gaccacgcag tggcggccgt
     1321 ggctgcccac caccaggccg tctagcagtt gggcgtcggc gcttgggccg ggcggctggc
     1381 gcgggcggct catcgggtgg tgtcggggct gcggcggggc ttggcgccgg ctgtggcgtg
     1441 ggccgacgat gctactataa ctatagctgc ttgcgctcgc cagacgggcg atggtgccga
     1501 tttttgtctt gaattcacgc ggactcgggg cgtgtcatac cggttgcacg cgcgccccca
     1561 tgcgggcgat gcgctcgctg gccggcgggt gcgagtaata gaagcgcacg tacagcgggt
     1621 cgggcgtcag cgtgctggcg ttgtccttgt agagct
//
LOCUS       DAOOOG010000361         1653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_95212_length_1653_cov_31.034418, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000361 DAOOOG010000000
VERSION     DAOOOG010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95212_length_1653_cov_31.034418"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1653)
                     /locus_tag="PK242_14410"
     CDS             complement(<1..>1653)
                     /locus_tag="PK242_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ95262.1"
                     /translation="HAVYYLHQQGVTRLDVVNFIAHGIRKTDTPEASKPAEGQPSPGE
                     AEEASEKSEKASPLEQFTQNLNQLARDGKIDPLIGRDYEVERTIQILCRRRKNNPLLV
                     GEAGVGKTAIAEGLAWRITEGTVPEILEGATVYALDMGALLAGTKYRGDFEQRLKGVL
                     KSLKDRPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGQLKCIGATTFTEYRG
                     IFEKDAALSRRFQKVDVVEPSVAETIEILKGLKSRFEEHHAVKYATAALQAAAELSAK
                     YITDRHLPDKAIDVIDEAGAAQRIAAASKRKKTIGKHEIEEIVAKIARIPPASVSNDD
                     RSKLQTLERDLKSVVFGQDQALDVLAAAVKMARSGLGKGDKPIGSFLFSGPTGVGKTE
                     AARQLAFIMGVELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAISKKPHCVL
                     LLDEIEKAHPDIFNVLLQVMDHGTLTDNNGRKADFRNVIIIMTTNAGAETMNKATIGF
                     TTQRQQGDEMADIKRLFTPEFRNRLDAIVSFKPLDEQVILRV"
BASE COUNT          216 a          518 c          574 g          345 t
ORIGIN      
        1 ccacgcgcag gatgacctgc tcgtccagcg gcttgaagct gacgatggcg tccaggcggt
       61 tgcggaactc gggcgtgaac aggcgcttga tgtcggccat ctcgtcgcct tgctggcgct
      121 gggtggtgaa gccgatggtc gccttgttca tggtctcggc gcccgcgttg gtggtcatga
      181 tgatgatgac gttgcggaag tcggccttgc gcccgttgtt gtcggtcagc gtgccgtggt
      241 ccatcacctg cagcagcacg ttgaagatgt cggggtgcgc cttctcgatt tcgtcgagca
      301 gcagcacgca gtgcggcttc ttgctgatgg cctcggtcag caggccaccc tggtcgaagc
      361 cgacatagcc gggcggcgcg ccgatcaggc ggctgacggc gtggcgctcc atgtactcgg
      421 acatgtcgaa gcggatcagt tcgaccccca tgatgaaggc cagctggcgc gccgcctcgg
      481 ttttgccgac gccggtgggg ccggagaaca ggaagctgcc gatcggcttg tcgcccttgc
      541 ccaggccgga gcgcgccatc ttgacggcag ccgccagcac gtcgagcgcc tggtcctggc
      601 cgaacaccac gctcttgagg tcgcgctcca gcgtctgcag cttgctgcgg tcgtcgttgc
      661 tgacgctggc cggcgggatg cgtgcgatct tggcgacgat ctcttcgatc tcgtgcttgc
      721 cgatggtttt cttgcgtttg ctggccgccg cgatgcgctg ggccgcgccg gcttcgtcga
      781 tcacgtcgat ggccttgtcg ggcaaatggc ggtcggtgat gtacttggcc gacagctcgg
      841 ccgcagcctg cagcgccgcg gtggcgtact tgacggcatg gtgctcctcg aagcggctct
      901 tgaggccctt gaggatttcg atggtttcag ccacgctcgg ctccaccaca tcgaccttct
      961 ggaagcgccg cgacagcgcc gcgtccttct cgaagatgcc acggtactcg gtgaaggtgg
     1021 tggcgccaat gcacttgagc tgtccgctcg acagtgccgg cttgagcagg ttgctggcat
     1081 ccagtgtgcc gccggaggcc gcgccggcac cgatcagggt gtggatctcg tcgatgaaca
     1141 ggatggcgtt gggccggtcc ttgaggctct tgagcacgcc cttgagacgc tgctcgaagt
     1201 cgccgcggta cttggtgccg gccagcagcg cgcccatgtc gagcgcgtag acggtggcgc
     1261 cttcaaggat ctcgggcacc gtgccttcgg tgatgcgcca ggccaggcct tcggcgatgg
     1321 cggtcttgcc gacacccgcc tcgcccacca gcagcgggtt gttcttgcgc cgccggcaca
     1381 ggatctggat ggtgcgctcg acctcgtagt cgcgcccgat cagcggatcg atcttgccgt
     1441 ccctggcgag ctgattcagg ttctgcgtga attgctccag cggcgaggcc ttctcgctct
     1501 tttcgctcgc ctcttcggcc tcgccggggc tgggctggcc ttcggcaggc ttgctggcct
     1561 ccggcgtgtc ggtcttgcgg atgccgtggg cgatgaaatt gaccacgtcc aggcgcgtga
     1621 cgccctgctg gtgcaggtag tagacggcgt gcg
//
LOCUS       DAOOOG010000362         1648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_95681_length_1648_cov_31.949780, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000362 DAOOOG010000000
VERSION     DAOOOG010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95681_length_1648_cov_31.949780"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1043
                     /gene="ruvB"
                     /locus_tag="PK242_14415"
     CDS             9..1043
                     /gene="ruvB"
                     /locus_tag="PK242_14415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOZ95263.1"
                     /translation="MPPGAAAPRLVSGAAASTREEALERALRPKLLDEYVGQAKAREQ
                     LEIFIGAARKRGEALDHVLLFGPPGLGKTTLSHIIAHELGVQLRQTSGPVLEKPKDLA
                     AILTSLEKHDVLFIDEIHRLSPVVEEILYPALEDYQIDILIGEGPAARSIKLDVQPFT
                     LVGATTRAGMLTNPLRDRFGIVSRLEFYTATELGRIVTRSAGLLKTPMDDEGGFEIAR
                     RSRGTPRIANRLLRRVRDYADVKGQGRITQDIAHAALAMLDVDPEGFDVMDRKLLEAV
                     IQRFDGGPVGLDNIAASIGEEPGTIEDVIEPYLIQQGYLQRTPRGRVATLAAWRHLGL
                     TPPQGAGGLF"
     gene            complement(1267..>1648)
                     /locus_tag="PK242_14420"
     CDS             complement(1267..>1648)
                     /locus_tag="PK242_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOZ95264.1"
                     /translation="ELPTDRRLRELAICTVALLTGAQYEYHHHAPDFLAAGGSQAELD
                     ALARVVARDARTDASDAALGAREQLVIRYAAQMTLDVRVDDALFEALRGHFDTTELVE
                     LTSAIATYNMVARFLVALGVTPEA"
BASE COUNT          253 a          583 c          582 g          230 t
ORIGIN      
        1 actttgccct gccgcccggc gccgcggcgc cgcgcctggt gtcgggcgct gccgcctcga
       61 cgcgagaaga ggcgctggag cgcgcgctgc ggcccaagct gctggacgag tacgtaggcc
      121 aggccaaggc gcgcgagcag ctcgagatct tcatcggcgc cgcccgcaag cgcggcgagg
      181 cgctggacca tgtgctgctg ttcggcccgc cggggctggg caagaccacg ctcagccaca
      241 tcatcgcgca cgagctgggc gtgcagctgc gccagaccag cggcccggtg ctggaaaagc
      301 ccaaggacct ggccgccatc ctgaccagcc tggaaaagca cgacgtgttg ttcatcgacg
      361 agatccaccg cctgtcgccg gtggtcgagg aaatcctcta ccccgcgctg gaggactacc
      421 agatcgacat cctgatcggc gaaggcccgg cggcgcgctc gatcaagctg gacgtgcagc
      481 ccttcaccct ggtgggcgcc accacgcgcg ccggcatgct gaccaacccg ctgcgcgacc
      541 gcttcggcat cgtctcgcgg ctggagttct acaccgccac cgagctgggg cgcatcgtca
      601 cgcgcagcgc ggggctgctg aagacgccga tggacgacga gggcggcttc gagatcgcgc
      661 ggcgctcgcg cggcacgccg cgcatcgcca accgcctgct gcgccgcgtg cgcgactacg
      721 ccgacgtcaa gggccagggc cgcatcacgc aggacatcgc gcacgccgcg ctggcgatgc
      781 tcgacgtcga tccggaaggc ttcgacgtga tggaccgcaa gctgctggag gcggtgatcc
      841 agcgcttcga cggcggcccg gtggggctgg acaacatcgc cgccagcatc ggcgaggagc
      901 cgggcaccat cgaggacgtg atcgagccct acctgatcca gcagggctac ctgcagcgca
      961 cgccgcgcgg gcgcgtggcc acgctggcgg cctggcgcca cctggggttg acgccgccgc
     1021 agggtgccgg cgggctgttc tgatccgggc gccgggcatc gacatcggac aaccgtgcgc
     1081 agagggcgca gaggtttcgc aaaaaaatcg gcatcggtgt gagcgacggc tcagggccac
     1141 tgccacccgg ctgccttgcg cccccattga ccgggacagc gatagcgccc acagtccttc
     1201 ggcggttctt ccgggtcctt tgcgaaacct ctgcgccctc tgcgtatgga ggtccggccg
     1261 caagcctcaa gcctccggcg tcactcccag cgccaccaga aagcgcgcca ccatgttgta
     1321 ggtggcgatg gcgctggtca gctcgaccag ctcggtggta tcgaagtggc cgcgcagggc
     1381 ttcgaacagg gcgtcgtcga cgcgcacgtc cagcgtcatc tgcgccgcat agcggatcac
     1441 cagttgttcc cgtgcgccca gcgccgcgtc gctggcgtcg gtgcgggcgt cgcgcgccac
     1501 cacgcgcgcc agggcatcga gctcggcctg gctgccgccg gcggccagga agtcgggcgc
     1561 gtggtggtgg tactcgtact gcgccccggt caacagcgcc acggtgcaga tggccagctc
     1621 gcgcaggcgg cggtcggtgg gcagctcg
//
LOCUS       DAOOOG010000363         1647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_95780_length_1647_cov_25.217337, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000363 DAOOOG010000000
VERSION     DAOOOG010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95780_length_1647_cov_25.217337"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PK242_14425"
     CDS             <1..551
                     /locus_tag="PK242_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HOZ95265.1"
                     /translation="ATLDDVMRAVWQRCRGGPMTEADFADTLADIGGRRFDAEIAAWV
                     HGTDELPLRELLQAQGVQVLEEPSQLAQTLGLRLGDGPGVTLKVVLDGGPAAAAGLAA
                     GDEWLGIELPARTRRSDASGWRLSRLDDLPLYAGAARQIVALVARDQRLLRLPLTLPH
                     PSTTWRLAPPVSADAAASWPGR"
     gene            536..>1647
                     /locus_tag="PK242_14430"
     CDS             536..>1647
                     /locus_tag="PK242_14430"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOZ95266.1"
                     /translation="MARPLNPRHERRGLLASTALALLLHALALALLGPLLRPPSILRQ
                     VAPPFYTRTITPEAPAAAPQQAAPVAQVSKPKRPVARIQKAPAAPVSVAPQAMAPASA
                     TPDTADAEAPLASDEPQAPSPAASAALAESPAPDDSLAARDPGGQDGAAAPPASAARA
                     AEPGFLADWPGDTRLTYTLGGNYRGELHGSARVLWQREGTRYHTAVQLDVGLLLSMHL
                     TSQGDITATGLQPAVYEEQVRQRRRGVRLDEDVRLHNGQRVARPAGVQDAASQFVELS
                     HRLATGQLSTEPGTAIPLWLARPGGVDEWTYDVVGEDTLHLPRLGAVRALHFRPRPLA
                     KPRGPIHAEIWFAPSLQYLPVRIRITQGPDTFMDLLA"
BASE COUNT          219 a          682 c          563 g          183 t
ORIGIN      
        1 gcgccacgct cgacgacgtg atgcgggctg tgtggcagcg ctgccgcggc ggcccgatga
       61 ccgaggccga cttcgccgac accctggccg acatcggcgg gcgccgtttc gacgccgaaa
      121 tcgccgcctg ggtgcacggc accgacgagc tgccgctgcg cgagctgctg caggcgcagg
      181 gtgtgcaggt gctggaggaa ccctcgcaac tggcgcagac gctgggcctg cgcctgggcg
      241 acggccccgg cgtgacgctc aaagtggtgc tcgacggcgg cccggccgcc gccgccggcc
      301 tggccgccgg cgacgaatgg ctgggcatcg agctgcccgc ccgcacccgg cgcagcgacg
      361 ccagcggctg gcgcctgagc cggctcgacg acctgccgct gtacgccggc gcggcgcgcc
      421 agatcgtcgc cctggtggcg cgcgaccagc gcctgctgcg cctgccgctg accctgccgc
      481 acccctccac cacctggcgg ctggcgcccc cggtcagcgc cgacgccgcc gcgtcgtggc
      541 ccggccgctg aacccacggc acgaacggcg cggcctgctg gccagcaccg cgctggcgct
      601 gctgctgcac gcgctggcgc tggcgctgct gggcccgctg ctgcgcccgc ccagcatcct
      661 gcgccaggtg gcgccgccct tctacacccg caccatcacc cccgaggcgc ccgcagcggc
      721 gccgcagcag gctgcgcccg tggcccaggt ttccaagcca aaacggcctg tagcgcgtat
      781 ccagaaagcg ccagcagctc ctgtttcagt agcaccccag gcgatggcgc cggcatccgc
      841 cacccccgac acggcagacg ccgaagcgcc gctggccagc gacgaacccc aggcgccctc
      901 acccgcggct tcggccgcgc tggccgagtc gccggcgccg gacgacagcc tcgccgcccg
      961 cgaccccggc ggccaggacg gcgccgccgc cccacccgcc agcgccgccc gcgccgccga
     1021 gcccgggttc ctggccgatt ggcccggcga cacccgcctg acctacaccc tgggcggcaa
     1081 ctaccgcggc gagctgcacg gcagcgcccg ggtgctgtgg cagcgcgaag gcacgcgcta
     1141 ccacacggcg gtgcagctcg acgtcggcct gctgctgtcc atgcacctga ccagccaggg
     1201 cgacatcacc gccaccggcc tgcaaccggc ggtctatgaa gagcaggtgc gccagcgccg
     1261 ccgcggcgtg cggctggacg aggacgtgcg cctgcacaac ggccagcggg tggcgcgccc
     1321 ggccggcgtg caggacgccg ccagccagtt cgtcgaactc agccatcgcc tggccaccgg
     1381 gcagctgagc accgagccgg gcaccgccat tcccctgtgg ctggcgcgcc ccggcggcgt
     1441 ggacgaatgg acctacgacg tcgtcggtga agacaccctg cacctgccgc gcctgggcgc
     1501 cgtgcgcgcc ctgcacttca ggccgcgccc gctggccaag ccgcgcggcc ccatccacgc
     1561 cgaaatctgg ttcgccccca gcctgcaata cctgcccgta cgcatccgca tcacccaggg
     1621 gccggacacc ttcatggacc tgctggc
//
LOCUS       DAOOOG010000364         1640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_96395_length_1640_cov_27.443533, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000364 DAOOOG010000000
VERSION     DAOOOG010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96395_length_1640_cov_27.443533"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /gene="sbcB"
                     /locus_tag="PK242_14435"
     CDS             <1..627
                     /gene="sbcB"
                     /locus_tag="PK242_14435"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652474.1"
                     /note="Exonucleolytic cleavage in the 3'- to 5'-direction
                     to yield nucleoside 5'-phosphates; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HOZ95267.1"
                     /translation="PEQVRARLFTSTADLPDGTTRLPIKGVHLNKSPIVVGQIKTLRP
                     ELAERWGIDFGVAASHLDHLRALPDLSALWPQVYARPGLAEPVDVDEDLYGGFLSDAD
                     RRRLERLRALEPGELAHARTGFDDERLPELLLRYRARNFPHTLNEAERARWLAHRTGR
                     LMDGEGGALTIAALFDRIDALVETVHDERGQAVLEALYAWAEHVAPEG"
     gene            complement(923..1498)
                     /locus_tag="PK242_14440"
     CDS             complement(923..1498)
                     /locus_tag="PK242_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOZ95268.1"
                     /translation="MPSLSPAPPPTGRARRRAWLARAARDGLLLLALLWAVQAWQTRD
                     VPSGAAPDFSAAAVLPPDRDAAMLSLAQWRAAHRGRPVALHFWAEWCGICRLEQHSVT
                     RLAADWPVLTVATQSGAAPAVHAVQARRELPWATVADPDGTLLTRYGLKAVPAFVVVA
                     PDGRLSSVSVGYTSEAGMRLRLWWAGLWAGG"
BASE COUNT          256 a          609 c          567 g          208 t
ORIGIN      
        1 cccgagcagg tgcgcgcgcg cctgttcacc agcaccgccg atttgcccga cggcacgacg
       61 cggctgccca tcaagggcgt gcacctgaac aaatcgccca tcgtggtcgg ccaaatcaag
      121 accctgcgcc ccgagctggc cgagcgctgg ggcatcgact tcggcgtcgc cgccagccac
      181 ctggaccacc tgcgtgcgct gcccgacctg agcgcgctgt ggccgcaggt ctatgcgcgc
      241 cccggcttgg ccgagccggt ggatgtggac gaagacctgt acggcggctt cctgagcgac
      301 gccgatcgcc gccgcctgga gcgcctgcgc gccctggagc ctggcgagct ggcccacgcg
      361 cgcaccggct tcgatgacga gcgcctgccc gagctgctgc tgcgctaccg tgcgcgcaac
      421 ttcccgcaca cgctgaacga ggccgagcgc gcccgctggc tggcgcatcg caccggccgg
      481 ctgatggacg gcgagggcgg cgcgctcacc atcgccgcgc tgttcgatcg catcgacgcg
      541 ctggtcgaga cggtgcacga tgagcgcggc caggcggtgc tggaagccct gtacgcatgg
      601 gccgaacacg tcgctccgga aggctgacat cgggcgttga cgaacgacgg atcgaatgac
      661 gaatgagctc gctgcgcatc gatgaccgat cgatcacaga ccatgccgcg cgtcgccggc
      721 gtcatgcatc atccctctga acatcagccg gaatactatc aaaacaatag cttgcggcgc
      781 ttgaccagca agcgccaaag ccctgtttga ctccgatgtt caggagcgcg cgctggcgac
      841 gccgaacccg ccgaaagcag gagggatgtt tccctgcgtc cttcgcgtcc ggcagtgcaa
      901 cagcccggca ccccggccca gatcagccgc cggcccacag gccggcccac cacagccgca
      961 gccgcatccc ggcttcgctg gtgtagccga cgctgacgct ggacaggcgc ccgtcgggcg
     1021 cgacgaccac gaaggccggc accgccttca ggccgtagcg cgtcagcagc gtgccgtcgg
     1081 ggtcggccac cgtggcccag ggcagttcgc gtcgggcctg cacggcgtgc accgccggcg
     1141 ccgcgccgga ttgggtggcc accgtcagca ccggccagtc ggcggctagg cgcgtcacgc
     1201 tgtgctgctc cagccggcag atgccgcacc actcggccca gaaatgcagc gccaccggcc
     1261 ggccgcgatg ggcggcccgc cactgcgcca ggctcagcat ggccgcgtcg cggtccggcg
     1321 gcagcacggc ggcggcgctg aaatcgggcg ctgcgccgct gggcacgtcg cgcgtctgcc
     1381 acgcctgcac ggcccacagc aaggccagca gcagcaggcc gtcgcgcgcc gcgcgtgcca
     1441 gccaggcgcg ccggcgcgcg cggccggtcg gcggcggggc gggggatagg gaaggcacgg
     1501 tgcggtgggc gacggcgaaa ccgatagtat tgacggtttc ccgccgccca gcgttttgcc
     1561 gccgggccgc ggtcccgtcc caccctcacc caggagcgcg ccgatgagca caccgaccaa
     1621 gtacctgctg caggaaagcc
//
LOCUS       DAOOOG010000365         1638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_96578_length_1638_cov_44.192040, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000365 DAOOOG010000000
VERSION     DAOOOG010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96578_length_1638_cov_44.192040"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1638
                     /locus_tag="PK242_14445"
     CDS             <1..>1638
                     /locus_tag="PK242_14445"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HOZ95269.1"
                     /translation="WGAVPLPSNHWRARGLPKIERNDPCHCGSGRKYKHCCASLGHVG
                     LPFDAQMLTALAIQHAPPEALDAASVRALPAHALAQAAMFWMEQDQPGQVVAVLGPLF
                     EQGQHLDGRHEPAFDILMEALLALGQQTRRFALARQIAEHRDKALATTARARLVSMLA
                     DQGNRAEAWTVFKQAQREAPDDPQLWHLELITLLSEGRVDEARLRGPLLAAKARRAGL
                     DELAHVLAELGEHGLSAIHDRLAHDADEPHATLAHDWLALCTQLPAELDAAAARALYR
                     IDITEPPRRRRATKAMDVTSLDGAMRWLRVRPAPALAALERRWQRSFVVGKPDTVWLD
                     GDADGLLAELPAAAEFLQREPQAWLSVAVLDDLLLAVRELLTNDPSPALRQSAWQLSQ
                     HALRLLRALLEPAVDGASASAQRLGVAWSHTPSRPLLRLLAQAISFGLDQQREVLPLL
                     EWSVALNPQDNHGWRAMLADAWIAADRHADALALLERYPDDFPPARHRRALALHALGR
                     HDEAAAELARAHAEYPAYLNALWPDVLDAPPPEDGPGL"
BASE COUNT          215 a          621 c          590 g          212 t
ORIGIN      
        1 tggggcgcgg tgccgctgcc ctcgaaccac tggcgcgcgc gcggcctgcc caagatcgag
       61 cgcaacgatc cgtgccactg cggatcgggc cgcaagtaca agcattgctg tgccagcctg
      121 gggcatgtcg gcctgccgtt cgacgcccag atgctcaccg cgctggccat ccagcacgcc
      181 ccgcccgagg cgctggacgc cgccagcgtg cgcgcgctgc cggcgcacgc gctggcgcag
      241 gcggccatgt tctggatgga gcaggaccag cccgggcagg tggtggcggt gctggggccg
      301 ctgttcgagc aagggcagca cctggatggg cgccacgaac cggcgttcga catcctgatg
      361 gaagccctgc tggcgctggg ccagcagacg cgccgcttcg cgctggcgcg ccagatcgcc
      421 gagcaccgcg acaaggcgct ggccaccacc gcacgcgcgc gcctggtcag catgctggcc
      481 gaccagggca accgtgccga ggcctggacg gtgttcaaac aggcccagcg cgaggccccc
      541 gacgatccgc agctgtggca cctggagctg atcacgctgc tgtccgaggg ccgcgtggac
      601 gaagcgcggc tgcgcggccc cctgttggcc gccaaggcgc gccgcgccgg tcttgacgaa
      661 ctggcacacg tgttggccga gctgggcgag cacggcctgt cggccatcca cgatcgcctg
      721 gcgcacgacg cggacgagcc gcacgccacg ctggcgcacg actggctggc cctgtgcacc
      781 cagttgccgg ccgagctgga cgcagccgcc gcccgggcgc tgtaccgtat cgacatcacc
      841 gagccgccgc gccgccggcg cgcgaccaag gcgatggatg tcacgtcgct ggacggtgcc
      901 atgcgctggc tgcgcgtgcg cccggcgccg gcgctggcgg cgctggagcg gcgctggcaa
      961 cgcagcttcg tggtcggcaa gcccgacacg gtgtggctgg acggcgatgc cgacggcctg
     1021 ttggccgagc tgcccgctgc cgccgaattt ctgcagcgcg agccgcaggc ctggctgagc
     1081 gtggccgtgc tcgacgatct gctgctggcc gtgcgcgagc tgctgaccaa tgacccatcg
     1141 ccggcgctgc gccagagcgc ctggcagctt tcgcagcacg ccctgcgcct gctgcgcgcc
     1201 ctgctcgaac cggcggtgga cggcgccagc gccagcgcgc agcgtctggg cgtggcgtgg
     1261 agccacacgc ccagccgtcc gctgctgcgc ctgctggcgc aggccatctc cttcgggctc
     1321 gatcagcagc gcgaggtgct gcccctgctc gaatggagcg tggccctgaa cccgcaggac
     1381 aaccacggct ggcgcgccat gctggccgac gcctggatcg ccgccgaccg ccatgccgac
     1441 gcgctggcgc tgctggagcg ctacccggac gacttcccgc cagcgcgcca ccgtcgcgcg
     1501 ctggcgcttc acgccctggg ccggcatgac gaggccgccg ccgagctggc ccgcgcccat
     1561 gccgagtacc cggcttacct gaacgccctg tggcccgacg tgctggacgc cccgccgccc
     1621 gaggacggcc ccggcctc
//
LOCUS       DAOOOG010000366         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_96730_length_1636_cov_27.549652, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000366 DAOOOG010000000
VERSION     DAOOOG010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96730_length_1636_cov_27.549652"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(573..767)
                     /locus_tag="PK242_14450"
     CDS             complement(573..767)
                     /locus_tag="PK242_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95270.1"
                     /translation="MGGLDLSPLGIVLLVVTGVLSFALGRWWSRGWRARRQARRAAEA
                     RKLETRQQRRAREREQAKRR"
     gene            complement(767..>1636)
                     /locus_tag="PK242_14455"
     CDS             complement(767..>1636)
                     /locus_tag="PK242_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOZ95271.1"
                     /translation="AKEFADPRRTLIQAEKKAVVEVKVQDEPVTVVVSEKGWVRARSG
                     HGHDAAGFAFKAGDGLYGTFECRTVDQLLVFGSNGRVYSVAVASLPGARGDGQPITTL
                     IDLEAGTQPLHYFAGPAGAALLLSGSGGYGFVARVEHMLSRNKSGKAFISIGAGEQVC
                     RPSLVALDPEQKTAPTQTGQAPAAIGSIAATHVACASVGGRILTFDIGELKLMEKGGR
                     GLTLIDLEPKDSLAGAAAYTRSVRLLGVGRGGKPREEQLEIRSLNNARAARGRKGRAA
                     DLGFKPHTIVRVE"
BASE COUNT          234 a          596 c          536 g          270 t
ORIGIN      
        1 agccgcagat cgtgcagcag ctgcagcagc agaagctggt gcagttccag caggacctgc
       61 gcaccaaggc caaggtcgag taagccgcgc cttgccgctt gcccaagggg gcggtcgctt
      121 caggcggccg cccctttttt gcgcctcata cggcgtcgcg ttcaaaaagc atgacccctg
      181 acccgtgggg catcgaagcg cggcgaggcc atccagcgtt tgggcctttt gaacctggaa
      241 acggcatttg accgctggtc aacttcgggc aggccgcatc gatcgatgcg gggtctggcg
      301 gctgcgatgg ccttcacctc ctgggtgagc acgctgcgca cccacccgca ccgcgctcgg
      361 cgcgccatgc ggcgttgctc cttcttgcgg gcaggagccc gcggcggcgt cggccttgca
      421 gggcgcagcc cagccagagg ctgggcccgt tcgcgccgtc ccaaggcgcg caggcgcctt
      481 tggagccgcg gcgctcaccc ttgcctggca caccgatcac ggcacgggcg ggctcgtcca
      541 acggccgttt ccaggttgaa cgcgcgttcg cgtcatcggc gcttggcctg ctcgcgctcg
      601 cgggcacggc gctgctggcg tgtttcgagc ttgcgcgcct cggccgcccg gcgcgcctgg
      661 cgtcgggcgc gccagccgcg cgaccaccag cgccccagcg caaagctgag cacgccggtc
      721 accaccagca gcacgatgcc caggggagaa agatccaggc cacccatcac tccacccgca
      781 cgatggtgtg gggcttgaag cccaggtcgg cggccctgcc cttgcgcccg cgcgcggcgc
      841 gggcgttgtt caggctgcgg atctccagtt gctcctcacg cggtttgccg ccgcggccga
      901 cgcccagcag gcgcacgctg cgcgtgtagg ctgcggcgcc ggccaggctg tccttgggct
      961 ccaggtcgat cagggtgagg ccacgcccgc ccttctccat cagtttcagc tccccgatat
     1021 caaaggtcag gatgcgccca cccaccgagg cacaggccac gtgtgtggct gctattgatc
     1081 caatagctgc tggcgcttgt ccagtctgcg tcggagccgt tttttgctct ggatccagcg
     1141 cgaccagcga gggcctgcac acctgctcgc cggcgccgat gctgatgaag gccttgccac
     1201 tcttgttgcg cgacagcatg tgctccaccc gcgccacaaa gccgtagccg ccactgcccg
     1261 acagcagcag cgcggcgccc gccggcccgg caaagtagtg cagcggctgg gtgcccgcct
     1321 ccagatcgat cagggtggtg atgggctggc catcgccgcg cgcgccgggc aggctggcca
     1381 ccgccacgct gtatacgcgg ccgttgctgc caaacaccag caactggtcc accgtgcggc
     1441 attcaaacgt gccgtacagg ccgtcaccgg ccttgaaggc aaagcccgcc gcgtcgtgcc
     1501 cgtggccgct gcgcgcgcgc acccagccct tttcgctgac caccaccgtg accggctcgt
     1561 cctgcacttt cacttcgacc acggcctttt tctcggcctg gatcagggtg cggcgggggt
     1621 cggcaaactc tttggc
//
LOCUS       DAOOOG010000367         1631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_97189_length_1631_cov_17.980330, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000367 DAOOOG010000000
VERSION     DAOOOG010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97189_length_1631_cov_17.980330"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1024)
                     /locus_tag="PK242_14460"
     CDS             complement(95..1024)
                     /locus_tag="PK242_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOZ95272.1"
                     /translation="MEIALIIVIIAALFIKLAIKVVPQQNAWVIERLGKYHGTLAPGL
                     NFIMPFLDRVAYRHGLKEIPMDVPSQVCITRDNTQLQVDGILYFQVTDPMRASYGASN
                     YVVAITQLAQTTLRSVIGKLELDKTFEERDMINAKVVEAIDEAALNWGVKVLRYEIKD
                     LTPPAEILRSMQAQITAEREKRAVIATSEGKRQEEINLAEGQKQAAIAKSEGEKQAAI
                     NNAQGQAAAITEVANATAVALARIGTAIRQPGGEQAVQLKVAERAVEAYSQLASQART
                     TLVVPSNMTEVSALISSAMKMVQAQQPGQDRAQ"
     gene            complement(1070..1495)
                     /locus_tag="PK242_14465"
     CDS             complement(1070..1495)
                     /locus_tag="PK242_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOZ95273.1"
                     /translation="MAESTLWWIAAGLLVAAELLTGTFYLLMLAIGGVAGALAAHAGL
                     GLSGQLVSGGVTGGVAVLISYLVRRRRPGDPSPRADRSVNLDVGETVQIDDWGADGTA
                     QIRYRGAPWIAVLRPGSTPRPGPHRVIELVGSRLLVEPV"
BASE COUNT          226 a          529 c          553 g          323 t
ORIGIN      
        1 gaacccgcgg tgggctatta acccacacac gctttccagg cgtgcgactt aaaccactca
       61 tccacctctc cgtggaaccg acgattgtag cgcgtcactg cgcccgatcc tggccgggtt
      121 gctgcgcctg caccatcttc atggccgagc tgatgagggc cgagacctcg gtcatgttgc
      181 tgggcaccac cagcgtggtg cgggcctggc tggccagctg gctgtaggct tcgacggccc
      241 gctcggccac cttcagctgc accgcctgct cgccgcccgg ctggcggatg gcggtgccga
      301 tgcgcgccag cgccacggcg gtggcgttgg ccacctcggt gatggcggcg gcctggccct
      361 gggcgttgtt gatggcggcc tgcttttcgc cttcggactt ggcgatggcg gcctgcttct
      421 ggccctcggc caggttgatt tcttcctggc gcttgccttc cgacgtggcg atgacggcgc
      481 gcttttcgcg ttcggcggtg atctgcgcct gcatcgagcg caggatttcg gccggcgggg
      541 tgaggtcctt gatttcgtag cgcagcacct tcacacccca gttcagcgcc gcttcgtcga
      601 tggcctcgac caccttggcg ttgatcatgt cgcgctcttc gaaggtcttg tccagctcca
      661 gcttgccgat cacgctgcgc agcgtggtct gcgccagctg ggtgatggcg accacgtagt
      721 tgctggcgcc gtagctggcg cgcatcgggt cggtgacctg aaagtaaagg atgccgtcca
      781 cctgcagctg agtgttgtca cgcgtgatgc agacctggct gggcacatcc atgggaattt
      841 ccttcaggcc gtggcggtag gcgacgcggt ccaggaaggg catgatgaag ttgagccccg
      901 gtgccagcgt gccgtgatac ttgcccagcc tttcgatgac ccaggcgttc tgctgcggca
      961 cgaccttgat cgccagcttg atgaacagcg cggcgatgat gacgatgatg agtgcgattt
     1021 ccatgggagt gtctttcttc tggctgtggt tgcgaaggca ggaggtggtt cagaccggct
     1081 cgaccagcag gcggctgccc accagttcga tcacgcggtg cggccccggc cggggcgtgc
     1141 tgccggggcg cagcacggcg atccagggtg cgccgcgata gcggatctgg gccgtgccgt
     1201 cggcgcccca gtcgtcgatc tgcacggttt cgccgacgtc gaggttgacg ctgcggtcgg
     1261 cgcgtggcga ggggtcaccc ggtcggcgcc ggcgcacgag gtagctgatc agcaccgcca
     1321 cgcccccggt cacgccgccg ctgacgagtt gcccgctcag ccccagcccg gcatgggcgg
     1381 ccagcgcgcc ggccacgccg ccgatggcca gcatcagcag gtagaaggtg ccggtcagca
     1441 actcggcggc caccagcagc ccggcggcaa tccaccagag ggttgattcg gccatgcgcg
     1501 cctccaatta atcgtttaga agtaccactg gagcgcgatt gtcgggcatg tgtccgccgc
     1561 ttccgcttgg tgccttggca attccgtaca cttgcgcgct gttttgccgc cttcggcagg
     1621 agtcaggtcc c
//
LOCUS       DAOOOG010000368         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_97543_length_1627_cov_25.326336, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000368 DAOOOG010000000
VERSION     DAOOOG010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97543_length_1627_cov_25.326336"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..91)
                     /locus_tag="PK242_14470"
     CDS             complement(<1..91)
                     /locus_tag="PK242_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HOZ95274.1"
                     /translation="MSDEQRRRNVRLGLILGTVALVFFIGFIVR"
     gene            complement(115..>1627)
                     /gene="ctaD"
                     /locus_tag="PK242_14475"
     CDS             complement(115..>1627)
                     /gene="ctaD"
                     /locus_tag="PK242_14475"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HOZ95275.1"
                     /translation="TLYLLFSFTMLMIGGVLALLIRAELFQPGLQVVNPELFNQLTTM
                     HGLIMVFGAIMPAFVGFANWMIPLQIGASDMAFARMNNFSFWLLIPAGIMLVGSFFMP
                     GGAPAAGWTLYAPLTLQMGMSMDTSIFAMHIMGASSIMGAINIIVTILNMRAPGMTLM
                     KMPMFCWTWLITAYLLIAVMPVLAGAITMTLTDRHFGTSFFNPAGGGDPVMYQHIFWF
                     FGHPEVYIMILPAFGIISQVVPAFARKKLFGYASMVYAVAAIAILSLIVWAHHMFTTG
                     MPVTGQLFFMYATMLIAVPTAVKIFNWIATMWRGSMTFETPMLFAVGFIFVFTIGGFT
                     GLIPAVAPIDTQIQDTYYIIAHFHYVLVAGSLFAMYSGFYYWAPKWTGVMYNETRGKV
                     HFWWSMLSFNLTFFPMHFLGLAGMPRRYADYPMQFADFNMVASIGAFLFGFAQVYFFL
                     AIVLPMMRGQGQKAPQRPWEGAEGLEWEVPSPAPFHTFENPPKLDATATRVVG"
BASE COUNT          346 a          487 c          543 g          251 t
ORIGIN      
        1 tgcgcacgat gaagccgatg aagaacacca gcgccaccgt gcccaggatc agccccaggc
       61 gcacgttgcg cctgcgttgc tcgtcggaca tcgcggtcga catttacagc ctgctcagcc
      121 caccacgcgc gtggcggtgg cgtccagctt cggcgggttt tcaaaggtgt ggaagggcgc
      181 gggcgacggc acttcccact ccaggccctc ggcaccttcc caggggcgct gcggcgcctt
      241 ctggccctgg ccgcgcatca tgggcagcac gatggccagg aagaagtaca cctgtgcaaa
      301 gccgaacagg aacgcgccga tcgacgccac catgttgaaa tcggcgaact gcatcgggta
      361 gtcggcatag cggcgcggca tgccggccag gcccaggaag tgcatcggga agaaggtcag
      421 gttgaacgac agcatcgacc accagaaatg gaccttgccg cgtgtttcgt tgtacatgac
      481 gccggtccac ttgggcgccc agtagtagaa gccggaatac atcgcgaaca gcgaaccggc
      541 caccagcaca tagtggaagt gggcaatgat gtagtaggtg tcctggatct gcgtgtcgat
      601 cggcgcgacg gccggaatca gcccggtgaa gcccccgatg gtgaacacga agatgaagcc
      661 gaccgcgaac agcatgggcg tctcgaacgt catcgaaccg cgccacatgg tggcgatcca
      721 gttgaagatc ttcacggccg tgggcaccgc gatcagcatg gtggcgtaca tgaagaacag
      781 ctggcccgtg accggcatgc cggtggtgaa catgtggtgc gcccacacga tcagcgacag
      841 gatggcgatg gccgccaccg catacaccat ggaggcgtag ccgaacagct tcttgcgcgc
      901 gaatgccggc accacctggc tgatgatgcc gaaggccggc aggatcatga tgtagacctc
      961 ggggtggccg aagaaccaga agatgtgctg gtacatcacc ggatcgccgc cgccggcggg
     1021 gttgaagaag ctggtgccga agtggcggtc ggtcagcgtc atggtgatgg cgccggccag
     1081 caccggcatc accgcgatca gcaggtaggc ggtgatcagc caggtccagc agaacatggg
     1141 catcttcatc agcgtcatgc cgggcgcgcg catgttcagg atggtgacga tgatgttgat
     1201 cgcacccatg atggagctgg cgcccatgat gtgcatcgcg aagatgctgg tgtccatcga
     1261 catgcccatc tgcagcgtca gcggggcgta cagcgtccag ccggcagcgg gcgcgccacc
     1321 gggcatgaag aacgagccca ccagcatgat gccggccggg atcagcagcc agaagctgaa
     1381 gttgttcatg cgcgcgaacg ccatgtcgct ggcgccgatc tgcagcggga tcatccagtt
     1441 ggcgaagccc acgaaggccg gcatgatggc gccgaacacc atgatcagcc cgtgcatggt
     1501 ggtcagctgg ttgaacagct cggggttgac cacctgcagg ccgggctgga acagctcggc
     1561 gcggatcagc agcgccagca cgccgccgat catcagcatc gtgaacgaga acagcaggta
     1621 cagcgta
//
LOCUS       DAOOOG010000369         1614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_98762_length_1614_cov_23.642078, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000369 DAOOOG010000000
VERSION     DAOOOG010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98762_length_1614_cov_23.642078"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..964
                     /locus_tag="PK242_14480"
     CDS             17..964
                     /locus_tag="PK242_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ95276.1"
                     /translation="MTAAPPLLDVQALSKSYAGTRVVDGVSLAIAPGECLGVIGPNGA
                     GKTTTIRLCLGLTAPDGGRIRYFVQPDGSALEMPRDALAIKARLGVVSQFDSLDPDFS
                     CAENLQVFGRYFGLPRAVLAERVPRLLEFAALGHKASARPGELSGGMKRRLSLARALI
                     NQPELLLLDEPTTGLDPQARHLMWERLQQLLADGRSILLTTHFMDEAERLCHRLLVID
                     HGRLIAEGRPRELIAAHLEPEVVEVYGQGALALAGSPLAAHAQRVEQSGETLFFYTRD
                     ARPLMQALAAHPQLRTLHRPANLEDLFLKLTGRQIREEG"
     gene            1119..>1614
                     /locus_tag="PK242_14485"
     CDS             1119..>1614
                     /locus_tag="PK242_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NodJ"
                     /protein_id="HOZ95277.1"
                     /translation="MNHPLTPSEPPPSHLASPWAPPRLSMRWWPVFARNLRVWRKLVI
                     PSLLANIGEPLLWLVAFGYGLGAMIGRVPTHGQDVPYILFLASGSVCMSAMNAASFEA
                     LYSAFSRMHVQKTWDGILNTPVQLDDVVLAEMLWAGFKALFSATVILAVIWALGISRA
                     PRTLL"
BASE COUNT          231 a          597 c          539 g          247 t
ORIGIN      
        1 tgcacaatcg ccgcccatga ccgctgcccc gccactgctc gacgtacagg ccctgagcaa
       61 gagctacgcc ggcacgcgcg tggtcgacgg cgtgtcgctg gccatcgcgc cgggcgaatg
      121 cctgggcgtg atcggcccca acggcgccgg caagaccacc accatccgcc tgtgcctggg
      181 cctgacggcg ccggacggcg gccgcatccg ctacttcgtg cagcccgacg gcagcgcgct
      241 ggagatgccg cgcgacgcgc tggccatcaa ggcgcggctg ggcgtggtgt cgcagttcga
      301 ctcgctcgac ccggacttca gctgcgccga gaacctgcag gtgttcggcc gctacttcgg
      361 cctgccacgc gccgtgctgg ccgagcgcgt gccgcgcctg ctcgaattcg ccgccctcgg
      421 ccacaaggcc agcgcccgcc cgggcgagct gtcgggcggc atgaagcggc gcctgtcgct
      481 ggcgcgcgcg ctcatcaacc agcccgaact gctgctgctg gacgagccca ccaccggcct
      541 ggacccgcag gcgcgccacc tgatgtggga gcgcctgcag cagctgctgg ccgacggccg
      601 gtcgatcctg ctgaccaccc acttcatgga cgaggccgag cgcctgtgcc atcgcctgct
      661 ggtgatcgac catggccgcc tgatcgccga gggccgcccg cgcgagctga tcgccgcgca
      721 cctggagccc gaggtggtcg aggtctacgg ccagggcgcg ctggcgctgg ccggctcgcc
      781 gctggcggcg cacgcccagc gggtggagca aagcggcgaa accctgttct tctacacccg
      841 cgacgcgcgc ccgttgatgc aggccctggc cgcccacccg cagctgcgca cgctgcaccg
      901 cccggccaac ctggaggacc tgttcctcaa gctgacgggc cggcagatcc gcgaagaggg
      961 gtagcgaccc cgcgcgtcaa accatgacga aatgacctcg cggcgcttcg atgacgcgcc
     1021 ttcgacgcat gacgggagac cttcatttcg tcatgccggc gccagccggc gtcatcgaag
     1081 cgcagcgaga tcatttcgtc atttgtcatc cgattgccat gaaccacccc ctcaccccat
     1141 cggaaccgcc gccaagccac ctggcatccc cctgggcccc gccgcgcctg tcgatgcgct
     1201 ggtggccggt gttcgcgcgc aacctgcgcg tgtggcgcaa gctggtcatt cccagcctgc
     1261 tggccaacat cggcgagccg ctgctgtggc tggtggcctt cggctatggg ctgggggcga
     1321 tgatcgggcg cgtgcccacg cacgggcagg acgtgcccta catcctgttc ctggccagcg
     1381 gctcggtgtg catgagcgcc atgaacgcgg ccagcttcga ggcgctgtat tcggccttct
     1441 cgcgcatgca cgtgcaaaaa acctgggacg gcatcctgaa cacccccgtg cagctggacg
     1501 acgtggtgct ggccgagatg ctgtgggccg gcttcaaggc gctgttttcc gccaccgtca
     1561 tcctggcggt gatctgggcg ctgggcatca gccgcgcgcc gcgcaccctg ctgg
//
LOCUS       DAOOOG010000370         1613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_98853_length_1613_cov_27.120668, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000370 DAOOOG010000000
VERSION     DAOOOG010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98853_length_1613_cov_27.120668"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PK242_14490"
     CDS             complement(<1..502)
                     /locus_tag="PK242_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOZ95278.1"
                     /translation="MKILVIGSGGREHALAWKLSTSPRVQQVYVAPGNGGTALDPVLK
                     NVPISDVAALRDWALAEKIALTVVGPEQPLAAGVVDDFRAHGLRIFGPTRAAAQLESS
                     KAFSKAFMQRHGIPTAAFQTFTDAAAARAYVERMGAPIVVKADGLAAGKGVVVAQTVA
                     EAHAAID"
     gene            complement(537..704)
                     /locus_tag="PK242_14495"
     CDS             complement(537..704)
                     /locus_tag="PK242_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ95279.1"
                     /translation="MRTPLAVLLLAAALAGCGGGELSWCIHSGSGSLAGGPNPPDCPP
                     DPKPDRSLQAS"
     gene            complement(712..1440)
                     /locus_tag="PK242_14500"
     CDS             complement(712..1440)
                     /locus_tag="PK242_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOZ95280.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRQRIWTRVVREIMVAARSGGGDPS
                     ANPRLRLAIDKAKAANMPADTIKRNIDKATGNLEGASYEEIRYEGYGIGGAAIIVDTM
                     TDNRVRTVAEVRHAFSKYGGNLGTEGSVAFQFRHVGQFFFAPGTSEDQVMEVALEAGA
                     DDVITDDDGAIEVLCDPGAFEAVKQALEAAGLTPEMAEVTLRADSSIDLAGDDAARMQ
                     KLLDVLEDLDDVQAVYHNASLELA"
     gene            1507..>1613
                     /locus_tag="PK242_14505"
     CDS             1507..>1613
                     /locus_tag="PK242_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOZ95281.1"
                     /translation="MADPILLAQHGDIQCVLLPGLANRHGLITGATGTG"
BASE COUNT          224 a          551 c          556 g          282 t
ORIGIN      
        1 cgtcgatggc ggcgtgcgcc tcggccacgg tttgcgccac caccacgccc ttgccagcgg
       61 ccaggccgtc ggccttgacg acgatgggcg cacccatgcg ttcgacgtag gcacgcgcgg
      121 cggcggcgtc ggtgaaggtc tggaaggcgg cggtcgggat gccgtggcgc tgcatgaagg
      181 ccttgctgaa ggccttggag ctttcgagct gcgccgccgc ccgggtgggg ccgaagatgc
      241 gcaggccgtg ggcacggaag tcgtccacca cgccggcggc cagcggctgc tcggggccga
      301 ccacggtaag cgcgatcttc tcggccaggg cccagtcgcg cagcgccgcg acgtcgctga
      361 tcggcacgtt cttcagcacc ggatccagcg ccgtgccgcc gttgccgggc gccacataga
      421 cctgctgcac gcgcggcgag gtgctgagct tccaggccag ggcgtgctcg cggccgcccg
      481 agccgatgac caggatcttc atcgaatcac tctaaaaata atagctgctt gcgcagtcag
      541 gacgcctgca atgaccgatc cggcttggga tcgggcgggc agtcgggagg gttggggccg
      601 cccgccagcg aacccgagcc gctgtggatg caccacgaca gctcgccgcc gccgcagccc
      661 gccagcgcgg cggccagcag cagcacggcg agcggcgtgc gcatcgcccg ttcaggccag
      721 ctccaggctg gcgttgtggt agaccgcctg cacatcgtcc aggtcttcca gcacgtcgag
      781 cagcttctgc atgcgggccg cgtcgtcgcc ggcgagatcg atgctgctgt cggcgcgcag
      841 cgtcacctcg gccatctcgg gcgtcaggcc ggcggcctcc agcgcctgct tgacggcctc
      901 gaaggcgccg gggtcgcaca gcacctcgat ggcgccgtcg tcgtcggtga tcacgtcgtc
      961 ggcgccggcc tccagggcga cttccatcac ctggtcctcg ctggtgccgg gggcgaagaa
     1021 gaactgcccc acatgacgga actggaacgc caccgaacct tcggtgccca ggttgccgcc
     1081 gtacttgctg aaggcgtggc gcacttcggc cacggtgcgc acgcggttgt cggtcatggt
     1141 gtcgacgatg atggcggcac cgccgatgcc gtagccctcg tagcggattt cctcgtagct
     1201 tgcgccttcg aggttgccgg tggccttgtc gatgttgcgc ttgatggtgt cggccggcat
     1261 gttggcggcc ttggccttgt cgatggccag ccgcaggcgc gggttggccg acgggtcacc
     1321 gccgccgctg cgcgcggcca ccatgatttc gcgcaccacg cgggtccaga tgcgctggcg
     1381 cttttcgtcc tggcgtccct tgcggtgttg aatgtttgcc catttgctgt ggccggccat
     1441 gacgcggaaa ccctctcgaa agtcgcttga taatgtggcg attttacttt tcacggacct
     1501 tgacccatgg ccgatccgat tctgcttgcc cagcacggcg acattcagtg cgtgcttttg
     1561 cccggcctgg ccaaccgcca cggcctgatc accggcgcca ccggcaccgg caa
//
LOCUS       DAOOOG010000371         1596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_100423_length_1596_cov_34.164179, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000371 DAOOOG010000000
VERSION     DAOOOG010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100423_length_1596_cov_34.164179"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..872
                     /locus_tag="PK242_14510"
     CDS             60..872
                     /locus_tag="PK242_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ95282.1"
                     /translation="MTPDTLLSARNLSVRFGGVLAVNDVSFDVRKGEVFTLIGPNGAG
                     KTTVFNLISRIYQPTGGEIDYAGPHGQVRLTQQAPHQVASLGIARTFQNIELFEHASV
                     LHNLLVGQHTHRRTGFWSDLLFTPAARTAELATRERVERVIDFLDLQHYREALVAGLP
                     YGVRKVVEMARALCAEPRLLLLDEPSSGLNVEETDDMAFWIQDIVHELGITVLMVEHD
                     MSLVSRVSDRVLAMNQGQVLAMGTPAEVQADARVAEAYLGSIDDVASLRRQA"
     gene            869..>1596
                     /locus_tag="PK242_14515"
     CDS             869..>1596
                     /locus_tag="PK242_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ95283.1"
                     /translation="MSEPILQLLNVESSYGPIKAIRGVSLRVEPGQIATVLGSNGAGK
                     STILKTISGIIDPGKGTIHFRGEDITARDPALIVQKGLSHVPEGREVFPLLSVHDNLL
                     MGAYTRKDRDGVARDMEQVYAYFPILRERAAQDAGLLSGGQQQMLAISRALMAAPEMI
                     LLDEPSLGLSPRLTKEIFEIVVRINRERGTTILLVEQNANMALNAADYGYVLENGRIV
                     MEGSCDVLREKEDIKEFYLGMKDAG"
BASE COUNT          292 a          512 c          534 g          258 t
ORIGIN      
        1 ttctaccgcc agggcatgtt caagcggcag aaatcgttcc agaaatcgga tcgcctcaaa
       61 tgacgcccga taccctgctt tcggcccgca acctgagcgt gcgcttcggt ggcgtgctgg
      121 ccgtcaatga cgtcagcttc gacgtgcgca agggcgaagt gttcaccctg atcggcccca
      181 acggcgccgg caagaccacg gtgttcaacc tgatcagccg catctaccag cccaccggcg
      241 gcgagatcga ctatgccggg ccgcacggtc aggtcaggct gacgcagcag gcgccgcacc
      301 aggtggcgtc gctgggcatc gcgcgcacct ttcagaacat cgaactgttt gagcacgcca
      361 gcgtgctgca caacctgttg gtggggcaac acacgcaccg gcgcaccggc ttctggagcg
      421 atctgctgtt cacaccggcg gcgcgcacag ccgagctggc cacccgtgag cgcgtcgaac
      481 gcgtgatcga cttcctcgat ctgcagcatt accgcgaagc gctggtggcg ggcctgccct
      541 atggcgtgcg caaggtggtt gagatggcgc gcgcgctgtg cgccgaaccg cgcctgctgt
      601 tgctggacga accttcgtcc ggcctgaacg tcgaggaaac cgacgacatg gcgttctgga
      661 tccaggacat cgtgcatgag ctgggcatca cggtgctgat ggtggagcac gacatgagcc
      721 tggtctcgcg tgtgtccgac cgcgtgctgg ccatgaacca gggccaggtg ctggccatgg
      781 gcacgcccgc cgaggtgcag gccgacgcca gggtggccga ggcctacctg ggctcgatcg
      841 acgacgtcgc ctcgctgcgg aggcaggcat gagcgagccg atcctgcagt tgctcaacgt
      901 cgagagctcg tacggcccga tcaaggcgat ccggggcgtc agtctgcgcg tggagccggg
      961 ccagatcgcc accgtgctgg gcagcaacgg cgccggcaag agcaccatcc tgaaaaccat
     1021 ctccggcatc atcgacccgg gcaagggcac gatccatttc aggggcgagg acatcaccgc
     1081 gcgcgacccg gcgctgatcg tgcagaaggg tctgtcgcac gtgcccgagg ggcgtgaggt
     1141 gtttcctctg ctgtcggtgc acgacaacct gctgatgggc gcctacaccc gcaaggatcg
     1201 cgacggcgtg gcgcgcgaca tggagcaggt ttatgcctac tttccaatcc tgcgcgagcg
     1261 cgctgcccag gatgccggcc tgctgtcggg tggccagcag cagatgctgg ccatcagccg
     1321 cgcgctgatg gcggcgcccg agatgatcct gctggacgag cccagcctgg gcctgtcgcc
     1381 cagactgacc aaggaaatct tcgagatcgt ggtgcgcatc aaccgcgagc gcggcaccac
     1441 catcctgctg gtggagcaga acgccaacat ggcgctcaac gccgccgact acggctacgt
     1501 gctggaaaac ggccgcatcg tgatggaagg cagctgtgac gtgctgcgcg agaaggagga
     1561 catcaaggag ttctatctcg gcatgaagga cgccgg
//
LOCUS       DAOOOG010000372         1590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_101013_length_1590_cov_25.138111, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000372 DAOOOG010000000
VERSION     DAOOOG010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101013_length_1590_cov_25.138111"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /gene="ccsA"
                     /locus_tag="PK242_14520"
     CDS             <1..488
                     /gene="ccsA"
                     /locus_tag="PK242_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOZ95284.1"
                     /translation="KLAPIWVFGIALCFVPIAFRQRGVGEAGGSYWLGYALVAALIVG
                     TILLGRRRIAARLPSFEVLDDVMYKSIAVGFAFFTIATVLGALWAAEAWGGYWSWDPK
                     ETWALIVWLNYAAWLHMRLMKGLRGTVASWWALAGLAVTTFAFIGVNMFLSGLHSYGS
                     L"
     gene            544..1539
                     /gene="msrP"
                     /locus_tag="PK242_14525"
     CDS             544..1539
                     /gene="msrP"
                     /locus_tag="PK242_14525"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HOZ95285.1"
                     /translation="MLIRSDRDAFQPPRGNPVTPRALYQGRRALLQAWAAGAAGAALA
                     PWAAREALAQTARPGKRAALPGARSALPGAVTMERLTAYDDVSSYNNFYEFGTDKGDP
                     VRQAQRLRTDPWSVRIEGLVHKPGRLTLEELLRLAPMEERIYRLRCVEGWSMVIPWVG
                     YSLGRLIERVQPMGSAKYIEFVTLADREVMPGVRGSVLDWPYTEGLRLDEAQHPLTLL
                     AFGLYGELLPPQNGAPLRLVVPWKYGFKSAKSIVALRFVEHEPRTAWNKAAANEYGFY
                     SNVNPEVDHPRWSQATERRIGEDGLFARKRKTLLFNGYEAQVGQLYAGMDLRKHY"
BASE COUNT          235 a          542 c          558 g          255 t
ORIGIN      
        1 acaagctggc gccgatctgg gtgttcggca tcgcgctgtg cttcgtgccg atcgccttcc
       61 gtcagcgcgg cgtgggcgag gccggcggca gctactggct cggctacgcg ctggtggcgg
      121 cgctgatcgt cggcactatc ctgctggggc gccggcgcat cgccgcacgc ctgcccagct
      181 tcgaggtgct ggacgacgtg atgtacaagt ccatcgcggt cggctttgcc ttcttcacca
      241 tcgccaccgt gctgggcgcg ctgtgggccg ccgaggcctg gggcggctac tggagctggg
      301 acccgaagga aacctgggcc ctgatcgtct ggctgaacta cgccgcctgg ctgcacatgc
      361 ggctgatgaa gggcctgcgc ggcaccgtgg cctcgtggtg ggcgctggcc gggctggcgg
      421 tgaccacctt cgccttcatc ggcgtcaaca tgttcctgtc gggcctgcac agttacggct
      481 cactttgaac gccaggccgc gcctggcatg cgcgagcagc tcttgtttca ggagtgaacg
      541 gcgatgctca tccgatccga ccgagacgct ttccagccgc cgcgcggcaa cccggtcacg
      601 ccgcgcgcgc tctaccaggg ccggcgcgcc ctgctgcagg cctgggcggc cggcgcggcc
      661 ggcgcggcgc tggcaccgtg ggcggcacgc gaggcgctgg cgcagacggc gcggcccggc
      721 aagcgggcgg cgctgcccgg cgcgcgctcg gcgctgcccg gcgcggtcac gatggagcgg
      781 ctgaccgcct acgacgacgt cagcagctac aacaacttct acgaattcgg caccgacaag
      841 ggcgacccgg tgcgtcaggc gcagcggttg cggaccgacc cctggtcggt gcgcatcgag
      901 ggcctggtgc acaagcccgg ccgcctgacg ctggaagagc tgctccggct cgcgccgatg
      961 gaagagcgca tctaccgcct gcgctgcgtc gagggctggt cgatggtgat tccctgggtc
     1021 ggctattcgc tcggccggct gatcgagcgc gtgcagccga tgggcagcgc gaaatatatc
     1081 gagttcgtca ccctggccga ccgcgaggtc atgcccggcg tgcgcggcag cgtgctggac
     1141 tggccctaca ccgagggcct gcgcctggac gaagcgcagc acccgctgac gctgctggcc
     1201 ttcggcctgt acggcgagct gctgccgccg caaaacggtg cgccgctgcg gctggtggtg
     1261 ccctggaagt acggcttcaa gtccgccaag tcgatcgttg ccctccgctt cgtcgagcac
     1321 gagccgcgca ccgcctggaa caaggccgcc gccaacgagt atggcttcta ttccaacgtc
     1381 aaccccgagg tggaccaccc gcgctggagc caggccaccg agcggcgcat cggcgaagac
     1441 ggcctgttcg ccagaaagcg caagacgctg ctgttcaacg gctacgaggc gcaggtgggt
     1501 cagctgtatg ccggcatgga cttgcgcaag cactattgaa cctagaaacc gcagttgacc
     1561 gcttgcaacc cttgggaatg ccgcatgaac
//
LOCUS       DAOOOG010000373         1569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_103019_length_1569_cov_37.037649, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000373 DAOOOG010000000
VERSION     DAOOOG010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103019_length_1569_cov_37.037649"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PK242_14530"
     CDS             complement(<1..440)
                     /locus_tag="PK242_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOZ95286.1"
                     /translation="MSYVSETRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGAVK
                     GRKASRHATSDWMEIEKQRGISVASSVMQMSYRDHVINLLDTPGHKDFSEDTYRVLTA
                     VDSALMVIDAANGVEAQTRRLIEVCRQRDTPIISFVNKMDREVR"
     regulatory      546..626
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            693..>1569
                     /locus_tag="PK242_14535"
     CDS             693..>1569
                     /locus_tag="PK242_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOZ95287.1"
                     /translation="MNAPLKNPVAQDSAIADITLADWGRKEIAIAETEMPGLMAIRAE
                     YAQHQPLKGARITGSLHMTIQTAVLIETLQALGAQVRWASCNIFSTQDHAAAAIAANG
                     TPVFAIKGESLADYWDYTHRIFEFGAAGSQAEGPNMILDDGGDATLLMHLGRQAESNP
                     GVLAAPKSEEERVLFAAIQARLKQDPSWYSRKCAEIIGVTEETTTGVHRLKEMSAAGT
                     LLFRAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKIAVVCGYGDVGKGSAQ
                     ALRALSAQVWVTEIDP"
BASE COUNT          282 a          522 c          517 g          248 t
ORIGIN      
        1 tcgcgcacct cgcggtccat cttgttgacg aagctgatga tgggtgtgtc ccgctggcgg
       61 cagacctcga tcaggcgcct cgtctgcgcc tccacgccgt tggccgcgtc gatcaccatc
      121 agcgccgaat cgacggcggt gagcacgcgg taggtgtcct cggagaagtc cttgtggccg
      181 ggcgtgtcga gcaggttgat gacgtggtcg cggtagctca tctgcatcac gctggaggcc
      241 accgagatgc cgcgctgctt ctcgatctcc atccagtcgc tcgtggcatg gcggctggcc
      301 ttgcggccct tgacggcgcc tgcgatctgg atcgcgccgg agaacagcag cagcttttcc
      361 gtcagcgtgg tcttgcccgc gtcggggtgg gaaatgatgg cgaaggtgcg gcggcgccgg
      421 gtttcggaaa cgtaagacaa gatgacgact ccggggcgcg agccgatcgg ggcatgcggc
      481 gcgccaaaaa accgtcaatt atcgttgccc ttcgtccctg agggagcgcc gctataatgc
      541 cgcccacttc cgaggagcgt tgcagcaccc ccggccagcg gggtgcgagg ctcggaattc
      601 gatcaaggca acgacgctca cccactgatc gcgtgtggtg agctgctcat cccgtcagtg
      661 gtttttcaaa agcattgcct ttggagcaaa ccatgaacgc acccctgaag aaccccgtgg
      721 cgcaggactc cgccatcgcc gacatcaccc tggccgactg gggccgcaag gaaatcgcca
      781 tcgccgagac cgagatgccc ggcctgatgg cgatccgcgc cgagtacgcc cagcaccagc
      841 cactcaaggg cgcgcgcatc accggcagcc tgcacatgac gattcagacc gccgtgctga
      901 tcgagaccct gcaggcgttg ggggcgcagg tgcgctgggc ctcgtgcaac atcttctcga
      961 cccaggacca cgccgccgcc gccatcgccg ccaacggcac gccggtgttc gccatcaagg
     1021 gagaaagcct ggccgactac tgggactaca cgcaccgcat cttcgaattc ggcgctgcgg
     1081 gcagccaggc cgaaggcccc aacatgatcc tggacgacgg cggcgacgcc acgctgctga
     1141 tgcatctggg ccgtcaggcc gagtccaacc ccggcgtgct ggccgccccc aagagcgagg
     1201 aggagcgggt gctgtttgcc gccattcagg cccggctcaa gcaggacccg agctggtaca
     1261 gccgcaagtg cgccgagatc atcggcgtga ccgaggaaac caccaccggc gtgcaccgcc
     1321 tgaaggagat gagcgccgcc ggcacgctgc tgtttcgcgc catcaacgtc aacgactcgg
     1381 tcaccaagag caagttcgac aacctgtacg gctgccgcga gtcgctggtg gacggcatca
     1441 agcgcgccac cgacgtcatg gtggccggca agatcgccgt ggtgtgcggc tacggcgacg
     1501 tcggcaaggg cagcgcgcag gcgctgcgcg cgctgtcggc ccaggtgtgg gtgaccgaga
     1561 tcgacccca
//
LOCUS       DAOOOG010000374         1569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_103021_length_1569_cov_24.505284, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000374 DAOOOG010000000
VERSION     DAOOOG010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103021_length_1569_cov_24.505284"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1569
                     /gene="alaS"
                     /locus_tag="PK242_14540"
     CDS             <1..>1569
                     /gene="alaS"
                     /locus_tag="PK242_14540"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOZ95288.1"
                     /translation="SDNFWMMADTGPCGPCSEIFYDHGPHIPGGPPGSPDEDGDRFIE
                     IWNHVFMQFDMKEDGSVVRLPAPCVDTGMGLERLAAILQHVHSNYEIDLFARLIAEAA
                     RVTGCTDLANPSLKVIADHIRATAFLVSDGVIPSNEGRGYVQRRIIRRAIRHGYKLGC
                     KAPFFHKLVPLLVELMGAAYPRLQAEAQRVQQVLKTEEERFFETLANGMDILDDALIA
                     GRKELPGHIAFKLHDTYGFPLDLTQDVCRERGVTVDVAGFDEAMAAQKAAGRAAGKFR
                     MDKAVDYDGPGNAFIGYEHLRGEARVLALYHEGSATPALREGQHGIVVLDRTPFYAES
                     GGQVGDQGTLWIEHHGEPAFVVQDTQKIKADVFGHHGTARKGGLKVGDAVIAHVDPDT
                     RAATMRNHSVTHLMHKALREVLGAHVQQKGSLVDADKTRFDFAHHGPVTPEQMADIER
                     RVNAEILANAATQARLMDIESAQQTGAMMLFGEKYGDTVRVLDIGSSRELCGGTHVAR
                     TGDIGLFKIVSEGG"
BASE COUNT          287 a          552 c          526 g          204 t
ORIGIN      
        1 agagcgacaa cttctggatg atggccgaca ccggcccctg cggcccgtgc agcgaaatct
       61 tctacgacca cggcccgcac atccccggtg gcccgcccgg cagcccggac gaggacggcg
      121 accgcttcat cgagatctgg aaccacgtgt tcatgcagtt cgacatgaag gaagacggca
      181 gcgtggtcag gctgcccgcg ccctgcgtgg acaccggcat gggcctggag cgcctggccg
      241 ccatcctgca gcacgtgcac agcaattacg agatcgacct gttcgcacgc ctgatcgccg
      301 aggccgcgcg cgtgacgggt tgcaccgacc tggccaaccc ctcgctgaag gtgattgccg
      361 accacatccg cgccaccgcc ttcctggtca gcgacggcgt gatccccagc aacgaagggc
      421 gcggctacgt gcagcggcgc atcatccgcc gcgccatccg tcacggctac aagctcggct
      481 gcaaggcgcc gttcttccac aagctggtgc cgctgctggt cgagctgatg ggcgcggcct
      541 acccgcgcct gcaggccgag gcccagcgcg tgcagcaggt gctcaagacc gaggaggagc
      601 gcttcttcga gaccctggcc aacggcatgg acatcctgga cgatgcgctg atcgccggcc
      661 gcaaggagct gcccgggcac atcgccttca agctgcacga cacctacggc ttcccgctcg
      721 acctgacgca ggacgtgtgc cgcgagcgcg gcgtcacggt cgacgtggcc ggtttcgacg
      781 aagccatggc ggcgcagaag gccgccggcc gcgcggccgg caagttccgc atggacaagg
      841 cggtcgacta cgacggcccc ggcaacgcct tcatcggcta cgaacacctg cgcggcgaag
      901 ccagggtgct ggcgctctac cacgaaggca gcgccacccc ggccctgcgc gaggggcagc
      961 acggcatcgt ggtgctcgac cgcacgccgt tctatgccga aagcggcggc caggtgggcg
     1021 accagggcac actgtggatc gagcaccatg gcgagccggc gttcgtggtg caggacacgc
     1081 agaagatcaa ggccgacgtg ttcggccacc acggcacggc gcgcaagggc gggctgaagg
     1141 tgggcgatgc ggtgatcgcc catgtcgacc ccgacacgcg cgccgccacc atgcgcaacc
     1201 atagcgtcac ccacctgatg cacaaggcgc tgcgcgaggt gctgggcgcg cacgtgcagc
     1261 agaagggcag cctggtcgat gccgacaaga cgcgcttcga cttcgcccac catgggccgg
     1321 tgacgcccga gcagatggcc gacatcgagc gccgcgtgaa cgccgaaatc ctggccaacg
     1381 ccgccaccca ggcgcgcctg atggacatcg aaagcgccca gcagaccggc gccatgatgc
     1441 tgttcggcga aaaatacggc gacaccgtgc gcgtgctcga catcggctca agccgcgagc
     1501 tgtgcggcgg cacgcacgtg gcgcgcaccg gcgacatcgg cctgttcaag atcgtcagcg
     1561 aaggcggcg
//
LOCUS       DAOOOG010000375         1558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_104130_length_1558_cov_23.363273, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000375 DAOOOG010000000
VERSION     DAOOOG010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104130_length_1558_cov_23.363273"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PK242_14545"
     CDS             complement(<1..923)
                     /locus_tag="PK242_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ95289.1"
                     /translation="MTVSPSLKKKLLVAFAAAVVAALAWWSWTRFTDSGPGEGFVNGN
                     GRIEATEIDVATKLPGRIEDILVREGDFVTAGQPLAKMRLETLEAQRDEALAMRQQAE
                     HSVTAAQAQVALREADVTAALALVGQRESELDAAQRRLTRSSTLSSEGAASIQELDDD
                     RARVRGAQATLAASKAQAAAARAAVEAARAQLVGAQSSVSAATASISRIEADIRDSEL
                     RSPRDGRVQVRVAQPGEVLGAGGRVLNLLDLSDVYITFFLPETVAGRVALGSEVRIIL
                     DAAPEYVIPATVSFVASAAQFTPKTVETASER"
     gene            complement(938..>1558)
                     /locus_tag="PK242_14550"
     CDS             complement(938..>1558)
                     /locus_tag="PK242_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOZ95290.1"
                     /translation="SELLVSRPDIISAEHQLRASNAHIGAARAAFLPRIALTGSFGTA
                     SSDLNGLFDSGSRAWTFMPTLSLPIFDGGRRRANLELSEVRRDIAVAGYEKTIQTAFR
                     EVADALSAKHWLAEQLTIQRANLEAQSERARLAKLRYDNGSAAFLEVLDAQRDLLGAQ
                     QQLVQARRALLSSQVALYAALGGGTLAAAGEAQGAASTPASTPSTR"
BASE COUNT          250 a          502 c          535 g          271 t
ORIGIN      
        1 cgctcgctgg cggtttccac cgtcttgggg gtgaactgcg ctgcgctggc gacgaaggaa
       61 acggtggctg gaatcacata ctcgggcgcg gcatccagaa tgatgcgaac ctccgaaccc
      121 agcgcaacgc ggccggccac ggtttcgggc aggaagaagg tgatgtacac gtccgacaga
      181 tcaaggaggt tcaagacacg tccgcccgct ccaagcacct cgccgggttg cgcgacacgc
      241 acctgaaccc gtccgtcgcg cggagaccgc agttcgctgt cgcggatgtc ggcttcgatg
      301 cggctgatac tggccgtagc ggcggacacg ctggactgcg cgccgacaag ctgcgccctg
      361 gcggcttcga ccgcggcgcg ggcagcggcg gcctgtgcct tgctggccgc gagggtcgct
      421 tgggcgcccc gtacgcgcgc ccggtcatcg tccagttcct ggatcgaggc ggctccctca
      481 ctcgacagcg tgctggagcg tgtcaggcgc cgttgcgcgg cgtccagttc ggactcgcgc
      541 tgaccaacca gagccaaggc ggcagtcacg tcggcttcac gcagcgccac ctgtgcctgc
      601 gccgcggtca ccgaatgctc agcctgctga cgcatggcca gggcctcgtc gcgctgcgct
      661 tccagcgttt ccagccgcat cttggccagt ggctggccgg ctgtgacgaa atcgccttca
      721 cgcaccagga tgtcttcgat gcggccggga agtttggtgg ccacgtcgat ctcggtggct
      781 tcgatgcggc cattgccgtt gacgaatcct tcgcccgggc cgctgtcggt aaacctcgtc
      841 cagctccacc aggccagcgc ggcaacaacc gctgcagcga aagcgaccag gagttttttc
      901 ttgagagagg gggagacagt catgggttcg gcgtgcctta acgggttgat ggggtggaag
      961 cgggagtcga ggccgcaccc tgtgcctcgc cggctgcggc gagggtgccg ccgccgagcg
     1021 cggcatacag cgccacctgg ctggacagca gcgcgcggcg cgcctgtacc agttgttgct
     1081 gggctcccag cagatcgcgt tgggcatcca gcacttccaa gaaggcggcc gagccgttgt
     1141 cgtagcgcaa cttggccagg cgtgcgcgtt cgctttgcgc ttccaggttg gctctctgga
     1201 tcgtcaactg ctcagccagc cagtgctttg cgctcagcgc atcggccacc tcgcggaagg
     1261 ccgtttgaat ggttttttcg tatccggcga cggcgatgtc gcggcgcact tcgctcagct
     1321 ccagattggc gcggcggcgc ccgccatcga agatgggcag cgacagggtg ggcatgaagg
     1381 tccaggcacg actgccggaa tcgaacaagc cattcaagtc ggagctggcc gtgccgaagc
     1441 tgccggtcag cgcaatgcgc ggcaaaaacg ccgcgcgggc cgcgccgatg tgggcattgc
     1501 tggcacgcaa ttggtgttcg gcggaaatga tgtccgggcg actgaccagc agctccga
//
LOCUS       DAOOOG010000376         1545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_105465_length_1545_cov_50.381208, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000376 DAOOOG010000000
VERSION     DAOOOG010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105465_length_1545_cov_50.381208"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PK242_14555"
     CDS             complement(<1..392)
                     /locus_tag="PK242_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOZ95291.1"
                     /translation="MNPLDAAIADPASVDAAIESRFSVRAFLSQPVPRPLIEDILRVA
                     SRAPSGTNAQPWKVYVLQGAVRDELVRQVSDAHEAIRQRPELAAEYREAYDYYPRQWV
                     EPYLSRRRHNGWSLYGLLGIERGDKDRM"
     gene            complement(389..826)
                     /locus_tag="PK242_14560"
     CDS             complement(389..826)
                     /locus_tag="PK242_14560"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOZ95292.1"
                     /translation="MSPTRPQPDPRDSYRAFRTITTRWMDNDQYGHVNNVVYYSWFDT
                     AVNAHLIEQGVLDTAAGATIGLVVETQCHYFAPLSFPQTIEAGIRVAHMGSSSVRYEV
                     GLFAQGAPLTAARGHFVHVYVDRATQRPVPLPEPLRQVLQALR"
     gene            complement(823..>1545)
                     /locus_tag="PK242_14565"
     CDS             complement(823..>1545)
                     /locus_tag="PK242_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOZ95293.1"
                     /translation="AQREIGFPWSEAMVERSRRSVGASIQATRVAQHEGVAANLAGGT
                     HHASAEAGSGYCVFNDIAVAARLLQAERTGGGQPARPPRIAVIDLDVHQGNGTASIFA
                     GDASVFTLSLHGEKNFPFRKVAGDLDVGLPDGTGDQVYLDALDLALVELDSRFRPDAV
                     FYLAGADPHEGDRLGRLKLSYDGLLARDRRVFDWAWTRRLPLTLCMGGGYGHDIGTTV
                     QVQVNTFGVALQYWGRWQNARP"
BASE COUNT          222 a          539 c          555 g          229 t
ORIGIN      
        1 tgcatgcggt ccttgtcgcc gcgctcgatg cccagcaggc cgtacagcga ccagccgttg
       61 tggcgccggc ggctcaggta gggctcgacc cactggcgcg ggtagtagtc ataggcttcg
      121 cggtattcgg ccgccagttc ggggcgctgc cggatcgcct cgtgggcatc gctgacctgg
      181 cgcaccagct cgtcgcgcac cgccccctgc agcacgtaca ccttccacgg ctgggcgttg
      241 gtgccgctgg gcgcgcggct ggccacgcgc aggatgtcct cgatcagcgg ccgcggcacc
      301 ggctgcgaca ggaaggcgcg caccgaaaag cggctttcga tcgccgcgtc cacgctggcc
      361 ggatcggcga tggcggcgtc gagcgggttc accgcagggc ctgcagcact tgccgcagcg
      421 gctcgggcag cggcaccggg cgctgcgtgg cacggtccac gtagacatgc acgaagtgcc
      481 cgcgcgcggc cgtcagcggt gcgccctggg cgaacaggcc gacctcgtag cgcacgctgg
      541 agctgcccat gtgtgccacg cgaatgccgg cctcgatggt ctgcggaaac gacagcggcg
      601 cgaaatagtg gcactgcgtc tccaccacca ggccgatggt ggcgccagcg gcggtgtcca
      661 gcacgccctg ctcgatcagg tgggcgttga cggcggtgtc gaaccacgag taatacacga
      721 cgttgttgac gtggccgtac tggtcgttgt ccatccagcg cgtggtgatg gtgcgaaagg
      781 cgcgatagct gtcgcgcggg tcgggttgcg ggcgggtcgg gctcatggac gggcattctg
      841 ccagcggccc cagtactgca gggcgacgcc gaaggtgttg acctgcacct gcacggtggt
      901 gccgatgtcg tgaccatagc caccgcccat gcacagcgtc agcggcagcc ggcgcgtcca
      961 ggcccagtcg aacacccggc gatcgcgcgc cagcaggccg tcgtagctga gcttcaggcg
     1021 ccccagacgg tcgccctcgt gcgggtcggc gccggccagg tagaacacgg cgtcggggcg
     1081 aaagcggctg tccagctcga ccagcgccag gtccagcgcg tccagataga cctggtcacc
     1141 cgtgccgtcc ggcaggccca cgtccagatc gcccgccacc ttgcggaacg gaaagttctt
     1201 ctcgccgtgc agcgacagcg tgaacacgct ggcgtcaccg gcgaagatgc tggccgtgcc
     1261 attgccctgg tgcacgtcga ggtcgatcac ggcgatgcgc ggcgggcgcg ccggctggcc
     1321 gccacccgtg cgctcggcct gcagcaggcg cgcggccacg gcaatgtcgt tgaacacgca
     1381 gtagccgctg ccggcttccg cgctggcgtg gtgcgtgccg ccggccaggt tggccgccac
     1441 cccctcgtgc tgggccacgc gcgtggcctg gatgctggcg ccgaccgaac ggcgcgagcg
     1501 ctcgaccatc gcctcgctcc acggaaagcc gatctcgcgc tgcgc
//
LOCUS       DAOOOG010000377         1523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_107789_length_1523_cov_27.467302, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000377 DAOOOG010000000
VERSION     DAOOOG010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107789_length_1523_cov_27.467302"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PK242_14570"
     CDS             complement(<1..715)
                     /locus_tag="PK242_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ95294.1"
                     /translation="MANYLLVHGAWGGAWYWRPVQHALIRAGHNAHAVTLTGLGERAH
                     LLSPAITLGTHIADVRQALAAEELSDCILVVHSYAGMLGTAVADREPPGTLRHLVYVD
                     AVVPKPGESWSGTHASGTRRERLHAAEISPTHSFPAPDPGVFGLSAADHAWVARRQVP
                     HPGHTYLEPLEFDPRRVARVPRTFIDCTQPKLATIDVIRQRVRDPAFWDGAWLPGSRI
                     VEMATGHDPMISETEAMTRL"
     gene            complement(831..>1523)
                     /locus_tag="PK242_14575"
     CDS             complement(831..>1523)
                     /locus_tag="PK242_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOZ95295.1"
                     /translation="MYATVGILAALRHAERTGAGQHLDMALLDAQVAMLANLGANHLV
                     AGTVPGRMGNAHVNIVPYQVFEVAPGDGGDKDFIILAVGNDGQFAKFCAVAGQPELAR
                     DERFATNAARVRHRAVLVPLLEAILKARAKADWLAALEVAGVPCGAINRLDEVFADPQ
                     VRARGMVDRWTHPLAAELRLVASPLKLSQTPVRTDRPPPLLGQHTDEVLTELLGADTK
                     RLAALRAARVIQ"
BASE COUNT          216 a          519 c          567 g          221 t
ORIGIN      
        1 gcaggcgtgt catcgcctcg gtctcgctga tcatcggatc gtgcccggtc gccatttcca
       61 cgatgcggct gccaggcagc caggcgccgt cccagaacgc cgggtcgcgc acgcgctggc
      121 ggatgacgtc gatggtggcc agcttgggct gggtgcagtc gatgaaggtg cgcggcacgc
      181 gcgccacccg gcgcgggtcg aactccagcg gctccaggta ggtgtggccg gggtgcggca
      241 cctggcgccg cgccacccag gcgtggtcgg ccgccgacag gccgaacacc cccgggtcgg
      301 gcgccggaaa gctgtgggtg ggactgatct cggccgcatg caggcgctcg cgccgggtgc
      361 cgctggcgtg tgtgccgctc cagctttcgc ccggcttggg caccacggca tcgacataca
      421 ccagatggcg cagcgtgccg ggcggttcgc ggtcggccac ggcggtgccc agcatgccgg
      481 cgtaggaatg caccaccagg atgcagtcgc tcagctcctc ggccgccagc gcctggcgca
      541 cgtcggcgat gtgcgtgccc agggtgatgg ccggcgacag caggtgggcg cgctcgccca
      601 gcccggtcag ggtgacggcg tgggcgttgt ggccggcgcg gatcagtgcg tgctgcaccg
      661 gccgccagta ccaggcgccg ccccaggcgc cgtgaacgag caggtagttg gccatggagg
      721 tacctcgaag agcgacggat gacggtggac cggacggtgt ctggatgagg tatcaaatcg
      781 gcccgcaggg catacccatc aatcgccagc agctatcata atgatagtat tcactggatc
      841 acccgggcgg cacgcagggc ggccagccgc ttggtgtctg cgcccagcag ctcggtcagc
      901 acctcgtcgg tgtgctggcc cagcagcggc ggcggccggt cggtgcgcac cggggtctgg
      961 ctcaacttga gcgggctggc caccagccgc agctcggccg ccagcgggtg cgtccagcgg
     1021 tcgaccatgc cgcgcgcgcg cacctgcggg tcggcaaaca cctcgtccag ccggttgatg
     1081 gcgccgcagg gcacgccggc cacttcaagc gcggccagcc agtcggcctt ggcacgggct
     1141 ttcaggatgg cttcgagcag cggcaccagc acggcgcggt ggcgcacgcg cgcggcgttg
     1201 gtggcaaagc gctcgtcgcg cgccagctcg ggctggccgg ccacggcgca gaacttggcg
     1261 aactggccat cgttgcccac cgccaggatg atgaagtcct tgtcaccgcc gtcgcccggc
     1321 gcgacttcga acacctggta gggcacgatg ttgacgtgcg cattgcccat gcgccccggc
     1381 acggtgccgg ccaccaggtg gttggcgccc agattggcca gcatggcgac ctgggcgtcc
     1441 agcagcgcca tgtccaggtg ctggccggcg ccggtgcgct cggcgtggcg cagcgccgcc
     1501 agaatgccga cggtggcgta cat
//
LOCUS       DAOOOG010000378         1520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_108102_length_1520_cov_25.044369, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000378 DAOOOG010000000
VERSION     DAOOOG010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108102_length_1520_cov_25.044369"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1520
                     /gene="ppsA"
                     /locus_tag="PK242_14580"
     CDS             <1..>1520
                     /gene="ppsA"
                     /locus_tag="PK242_14580"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOZ95296.1"
                     /translation="GGKNASLGEMISQLPQGVRVPTGFATTAHAFREFLKHEGLAERI
                     SARLAALDVDDVRALAKAGAEIRAWVENQPFPADLEKAVRDAFAQLSGGNAQASFAVR
                     SSATAEDLPDASFAGQQETFLNVVGIEDVLHKMKEVFASLYNDRAISYRVHKGFAHDV
                     VALSAGVQRMVRSDLGAAGVMFTIDTESGFDQVVFITSSYGLGETVVQGAVNPDEFYV
                     HKPMLKAGKKALIRRNLGSKLIQMVFATPEERAASGRLVKTCDVPAELRNRYSLSDAE
                     VEQLAHYALVIEQHYGRPMDIEWGKDGQDGQLYILQARPETVKSQQGSTELRYKLKGS
                     SAVLAEGRAIGQKIGTGPVRLVHNIAEMDKVQPGDVLVTDMTDPNWEPVMKKASAIVT
                     NRGGRTCHAAIIARELGIPAVVGCGDATARLKDGTLVTVSCAEGDTGHIYDGLLETEV
                     TEVRRGEMPPIATKIMMNVGNPQLAFDFAQLPNEGVGLARLEFIINNNIGVHPKAIL"
BASE COUNT          285 a          507 c          510 g          218 t
ORIGIN      
        1 ggcggcaaga acgccagcct cggcgagatg atttcgcagc tgccccaggg cgtgcgcgtg
       61 cccaccggct ttgccaccac ggcgcacgcc ttccgcgaat ttctgaagca cgaaggcctg
      121 gccgagcgca tcagcgcccg cctggcggcc ctcgatgtgg acgacgtgcg cgcgctggcc
      181 aaggccggcg ccgagatccg cgcctgggtg gaaaaccagc ccttccccgc cgacctggaa
      241 aaggccgtgc gcgacgcctt tgcccagctg tccggcggca acgcccaggc cagctttgcc
      301 gtgcgcagct cggccacggc cgaggatctg cccgacgcct cgtttgccgg ccagcaggag
      361 actttcctga acgtcgtcgg catcgaggac gtgctgcaca agatgaagga ggtgttcgcc
      421 tcgctgtaca acgaccgcgc catctcctac cgcgtgcaca agggctttgc ccacgacgtg
      481 gtggcgctgt cggcgggcgt gcagcgcatg gtgcgctcgg acctgggcgc ggccggcgtg
      541 atgttcacca tcgacactga aagcggtttc gaccaggtag tcttcatcac cagcagctat
      601 ggcctgggcg agaccgtggt ccagggcgcc gtcaacccgg atgagttcta tgtgcacaag
      661 cccatgctca aggccggcaa gaaggcgctg attcgccgca acctgggcag caagctgatc
      721 cagatggtgt ttgccacgcc cgaggaaagg gcggccagcg gtcgcctggt caagacctgc
      781 gacgtgccgg ccgagctgcg caaccgctac tcactcagcg atgccgaggt cgagcagctg
      841 gcgcattacg ccctggtgat cgagcagcac tatggccgcc cgatggacat cgaatggggc
      901 aaggacggtc aggacggcca actctacatc ctgcaggcgc ggcccgagac ggtcaaaagt
      961 cagcagggca gcaccgagct gcgctacaag ctcaagggca gcagcgcggt gctggccgaa
     1021 ggccgtgcca tcggccagaa gatcggcacc ggtccggtgc gcctggtgca caacatcgcc
     1081 gaaatggaca aggtgcagcc gggcgacgtg ctggtgaccg acatgaccga cccgaactgg
     1141 gagccggtga tgaagaaggc cagcgccatc gtcaccaacc gcggcgggcg cacctgccac
     1201 gccgccatca tcgcgcgcga gctgggcatt ccggcggtgg tcggctgcgg cgacgccacc
     1261 gcgcgcctga aggacggcac gctggtgacg gtgagctgcg ccgaaggcga caccggccac
     1321 atctacgacg gtctgctgga aaccgaggtc accgaggtgc gccgcggcga gatgccgccg
     1381 atcgccacca agatcatgat gaacgtgggc aacccgcagc tggcgttcga ctttgcccag
     1441 ctgccgaacg aaggcgtggg tctggcgcgg ctggagttca tcatcaacaa caacatcggc
     1501 gtgcacccca aggccatcct
//
LOCUS       DAOOOG010000379         1519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_108201_length_1519_cov_25.439208, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000379 DAOOOG010000000
VERSION     DAOOOG010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108201_length_1519_cov_25.439208"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1450
                     /locus_tag="PK242_14585"
     CDS             149..1450
                     /locus_tag="PK242_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate carboxypeptidase"
                     /protein_id="HOZ95297.1"
                     /translation="MVLLRRFSAAAAAVSCLVGLGGCAQPGPGASAPAAQVSAAAARS
                     PDQALLAAAQAEKAATIKTLERLVNIETGTGDAQGMAAMAELLEGELRALGATVTRHK
                     AAEGRVGDNIVGRIPGKGGRKLLLMAHMDTVYVRGTLAKAPFRIEGNKAYGPGIADDK
                     GGVAVILHTLRLLKARGFDGFGELTVMFNTDEEMGSFGSRALIQQLAAQADEVLSFEP
                     TAAGREGLTLGTSGIVYYRVGVKGLAAHAGANPELGVNAAVEGADIIARTMDLDDKAR
                     DLRFNWTIFQSGDKPNIVPDAAKLEANIRYARPEDLDQLLKTLEARIQGKKKLPKSEI
                     TIAPERGRPAFNAGEGGRRLVDQTVAIYKEAGGTLTVIPRTGGGTDAAYAALSGKPVI
                     ESLGLPGFGYHSNQAEYVDLDAIPRRLYLATRLIMDLSSAK"
BASE COUNT          249 a          539 c          538 g          193 t
ORIGIN      
        1 gcgcagaccg tgccggtgct gcaggccctg gcggcggcgg tgcgggcgcg ccgcgccact
       61 tgatcggctc gcgccgacgg cccccggcgg gcgccggcgg cggcgctaca gtgtggtgtc
      121 cacccgatgg gttcacgagg agcatcacat ggttctgcta cgtcgattca gcgccgccgc
      181 ggccgccgtc agttgcctgg tagggctggg cggttgcgcc cagccgggcc cgggcgccag
      241 cgcacccgcg gcgcaggtca gcgcagcggc ggctcgcagt ccggatcagg ccctgctggc
      301 cgccgcgcag gctgaaaagg cagccaccat caagacactg gagcgcctgg tcaacatcga
      361 aaccggcacc ggcgacgccc agggcatggc cgccatggcc gagctgctgg agggcgagct
      421 gcgcgcgctc ggcgccaccg tcacgcgcca caaggcggcc gagggccggg tgggcgacaa
      481 catcgtcggc cgcatcccgg gcaagggcgg caggaagctg ctgctgatgg cgcacatgga
      541 cacggtgtac gtcaggggca cgctggccaa ggcccccttc cgcatcgagg gcaacaaggc
      601 ctacggtccg ggcatcgccg acgacaaggg cggcgtggcc gtgatcctgc acacgctcag
      661 gctgctgaag gcccgcggct tcgacggctt tggcgagctg acggtgatgt tcaacaccga
      721 cgaggagatg ggctccttcg gctcgcgcgc gctgatccag cagctggccg cgcaggccga
      781 cgaggtgctg tcgttcgagc ccacggcggc cgggcgcgag gggctgaccc tcgggacatc
      841 cggcatcgtc tattaccgcg tcggcgtcaa gggcctggcg gcgcacgccg gcgccaaccc
      901 cgagctgggc gtgaacgccg cggtggaagg cgccgacatc atcgcgcgca cgatggatct
      961 ggacgacaag gcgcgcgacc tgcgcttcaa ctggaccatc ttccagtccg gcgacaagcc
     1021 caacatcgtt cccgacgccg ccaagctgga agccaacatc cgctacgccc ggcccgagga
     1081 cctggaccag ctgctgaaga cgctggaggc gcgcatccag ggcaagaaga agctgcccaa
     1141 gtccgagatc accattgcgc ccgagcgtgg tcgtccggcc ttcaacgcgg gcgagggcgg
     1201 gcgccggctg gtcgatcaga ccgtggccat ctacaaggag gcgggcggca ccctgaccgt
     1261 gatcccgcgc accggcggcg gcaccgacgc ggcctatgcc gcgctgtcgg gcaagcccgt
     1321 catcgaaagc ctgggcctgc ccggcttcgg ctaccacagc aaccaggccg aatacgtcga
     1381 cctggacgcc atcccgcggc gcctgtacct ggccacgcgg ctgatcatgg atctgtccag
     1441 cgccaagtga gtccaccatg ctgctgaccg aagaccaacg cctgatccgc gacgccgtgc
     1501 gcgccttcgc gcaggagca
//
LOCUS       DAOOOG010000380         1506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_2629
            NODE_109695_length_1506_cov_20.660234, whole genome shotgun
            sequence.
ACCESSION   DAOOOG010000380 DAOOOG010000000
VERSION     DAOOOG010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864704
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:26:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,888
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 0 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109695_length_1506_cov_20.660234"
                     /isolate="AMR_MDS_2629"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PK242_14590"
     CDS             <1..996
                     /locus_tag="PK242_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOZ95298.1"
                     /translation="VLQFGDHGGAVGYLVGEENRGLEYMFIMMNAARYAVGVQGIAIA
                     ERAYQKAVQYAGERVQSRPVDGSTPGAATIIHHPDVRRMLMTMRALTEGCRAMASTAA
                     AAYDAAHHHPDADVRRQNQVFYEFLVPLVKGYSTEMSLEATNLGVQVHGGMGFIEETG
                     AAQYYRDAKILTIYEGTTAIQANDLVGRKTARDGGQTARAVAAQIEATERQLAGGSSD
                     AQAVARRLATARQAFLDVVDFMAAQARANPNAAYAGSVPYLMLTGNLVAGWQLGRALI
                     AAEQQKAAGHDAAFMTAKIATARFYADHILTRVPGVRDAIVEGADSVTALAPDAF"
     gene            1087..>1506
                     /locus_tag="PK242_14595"
     CDS             1087..>1506
                     /locus_tag="PK242_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOZ95299.1"
                     /translation="MSRLPPVLQRLKFPVIGSPLFIISNPKLVIAQCTSGVVGSMPAL
                     NARPAAQLDEWLAEITEALAAWDRAHPDNPAAPFAINQIVHKSNDRLEHDMQLCAKYK
                     VPIIITSLGAREDVNHAVHGWGGVVLHDVINNVFAHKA"
BASE COUNT          268 a          518 c          499 g          221 t
ORIGIN      
        1 gtgctgcagt tcggcgacca cggcggcgcc gtcggctacc tggtcggcga agaaaaccgc
       61 ggcctcgaat acatgttcat catgatgaac gccgcccgct atgcggtcgg tgtgcagggc
      121 atcgcgattg ccgagcgggc ctaccagaag gcggtgcagt acgccggcga acgggtgcag
      181 agccgcccgg tcgatggcag cacgccgggc gccgccacca tcatccacca ccccgacgtg
      241 cgccgcatgc tgatgaccat gcgcgccctg accgagggtt gtcgcgccat ggccagcacg
      301 gcggcagcgg cctacgatgc ggcccaccac cacccggatg ccgacgtgcg ccggcagaac
      361 caggtcttct atgaatttct ggtgcctctg gtcaagggct acagcaccga gatgagcctg
      421 gaggccacca acctgggcgt gcaagtgcac ggcgggatgg gcttcatcga ggaaaccggc
      481 gccgcgcagt actaccgcga tgccaagatc ctgaccatct acgaaggcac gaccgccatc
      541 caggccaacg acctggtggg ccgcaagacg gcgcgcgatg gcggccagac cgcccgggcg
      601 gtcgcggcgc agatcgaagc caccgagcgt caactggccg gcggcagcag cgatgcccag
      661 gccgtggcgc gccggctggc gacggcccgg caggcgtttc tggacgtggt cgacttcatg
      721 gccgcacagg cgcgcgccaa cccgaacgcc gcctacgccg gcagcgtgcc ctacctgatg
      781 ctgaccggca acctggtggc cggctggcaa ctgggccgcg cgctgatcgc ggccgagcag
      841 cagaaggccg ccggccacga cgccgccttc atgacggcca agatcgccac cgcgcgcttc
      901 tacgccgacc acatcctcac gcgcgtgccg ggggtgcgcg acgccatcgt cgaaggcgcc
      961 gacagcgtga ccgcgctggc gccggacgcg ttctgaattt gctactgaat tcatagctgc
     1021 tggcgctcgt agaacgggcg ctggaggcct tttttatatt caatctggtt tcctgagagg
     1081 atcgccatgt cccgtctgcc accggtgctg caacgcctga agttccccgt catcggatcg
     1141 ccgctgttca tcatcagcaa ccccaagctg gtgatcgcgc aatgcacttc gggcgtggtg
     1201 gggtcgatgc cggcgctcaa tgcgcgcccg gcggcgcagc tggacgaatg gctggccgag
     1261 atcaccgagg cgctggccgc gtgggatcgc gcccacccgg acaacccggc cgcgcccttt
     1321 gccatcaacc agatcgtgca caagagcaac gaccggctgg agcacgacat gcagttgtgc
     1381 gccaagtaca aggtgccgat catcatcacc agcctgggcg cgcgcgagga cgtcaaccac
     1441 gccgtgcatg gctggggcgg cgtggtgctg cacgacgtca tcaacaacgt gtttgcccac
     1501 aaggcg
//
